LOCUS       DASZAK010000001         3436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_425938, whole genome shotgun sequence.
ACCESSION   DASZAK010000001 DASZAK010000000
VERSION     DASZAK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425938"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..556
                     /gene="rpsC"
                     /locus_tag="VGT44_00005"
                     /pseudo
     CDS             1..556
                     /gene="rpsC"
                     /locus_tag="VGT44_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003757022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            989..1420
                     /gene="rplP"
                     /locus_tag="VGT44_00010"
     CDS             989..1420
                     /gene="rplP"
                     /locus_tag="VGT44_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV2579205.1"
                     /translation="MLLAPSRSKYRKQHRGRMKGTAQRGNSVAFGEFALQAMEPCWMT
                     ARQIESARIALTRFMKRGGKVWIRVFPDKPVTAKPAETRMGSGKGAVDHYVAVIKPGR
                     IIFEIGGVSEEIAKEAARLASHKLPIKTRFIAKGEAESAEA"
     gene            1407..1616
                     /gene="rpmC"
                     /locus_tag="VGT44_00015"
     CDS             1407..1616
                     /gene="rpmC"
                     /locus_tag="VGT44_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV2579206.1"
                     /translation="MPKLKERRKQIAEMTIGEAQRELKDLREKLFNLRLQQQRGEIKN
                     NRIFTQTKKDVARVLFRLKQLETEE"
     gene            1613..1915
                     /gene="rpsQ"
                     /locus_tag="VGT44_00020"
                     /pseudo
     CDS             1613..1915
                     /gene="rpsQ"
                     /locus_tag="VGT44_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
     gene            2013..2381
                     /gene="rplN"
                     /locus_tag="VGT44_00025"
     CDS             2013..2381
                     /gene="rplN"
                     /locus_tag="VGT44_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV2579207.1"
                     /translation="MIQPQTRLKVADNTGAKEIMCIRVMSGSAKRYAGIGDVIKASVK
                     VAAPNGQVKKGEVVDAVIVRVSKEYARPDGSHIRFDENAAVIIVAGNNPRGTRIFGPV
                     ARELRDRNFMKIISLAPEVL"
     gene            2526..2870
                     /gene="rplX"
                     /locus_tag="VGT44_00030"
     CDS             2526..2870
                     /gene="rplX"
                     /locus_tag="VGT44_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV2579208.1"
                     /translation="MNIKKGDTVLIITGKDRGKQGTVSRALPQVSKVIVEGLNIAKKH
                     VRPQGQTRQGGIIEKAMPLHVSNTMLICTECGEPTRVAHDRRPMGVDQKVRLVRVCKK
                     CHKIIEDRTRSS"
     gene            2873..3415
                     /gene="rplE"
                     /locus_tag="VGT44_00035"
     CDS             2873..3415
                     /gene="rplE"
                     /locus_tag="VGT44_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV2579209.1"
                     /translation="MASRLKEKYQKEVVPALHKEFNYKNPMQVPTVNKVVVNIGMGEA
                     IQNAKAMDAAVSDLATITGQRPVITRAKHSVAAFKLREGMQIGCMVTLRGERMYHFLD
                     KLMNVALPRLRDFQGVSADAFDGRGNYTLGLREQLVFPEIDYDKIDKVRGMEVSIVTT
                     ARTDEEGRQLLKLMGMPFKK"
BASE COUNT          860 a          902 c         1069 g          605 t
ORIGIN      
        1 tttcaaactg cgcaagatga ttcagaagga gctggccaac gcctcggttt cgcgcatcga
       61 gattgaacgt gctgccgcga accaggtgtc gctcaccatt catacggccc ggccaggcat
      121 cgtgatcggc aaaagcggcg aaaaggttga caagctgcgc gccaccctgg aaaaagagac
      181 caggaagcgc gtcaggctca acatcctgga aatccgccag cccgaactgg atgcctacct
      241 ggtagcgcgc agtgtggcgg agcagttgga gaagcgtgtc tcgttccggc gcgctatgaa
      301 gcaggcggtg cagaagacca tgcgcgccaa cgcgaagggc attcgtatag tcgtcggcgg
      361 tcgcctgggt ggggcggaaa ttgctcgctc tgaaaaagaa gttgagggca agattccgct
      421 gcataccctg cgcgccgaca tcgattacgg cctggcggag gcgcatacga ccttcggcgt
      481 aatcggtgtg aagatctgga tttataaggg cgatattttg ccgagcaagc gccgcgaggc
      541 gttggaaggg acgcaagaga gcgcatcttt cacgcagacc gagcgcggcg aaggtgatcg
      601 acgcccccca cgtggagatc gcggcccgcg cggcggtgag cgcagtgatc gtggcgctcc
      661 acgcggtgat cgtccggctc gccagggtgg cgaacgttct acccgcccgc aaggcgaacg
      721 cagcccgcgc ccacagggtg aacgcccggc tcgttcgcag gcagctacgg gcgaccgccc
      781 gccgcgcccg ccgcgcccgc aaggtgaacg ccctgcccgg ccagcgcgcg ctccgcggac
      841 agagcagcca gccgagaagc cggcggctca gccagccgag caagctccgg ttgtgcagac
      901 gagcgaaccg atgccaacgg cagagagtac accggtccag acgccggaga caggcgctcc
      961 tgagagcgaa tagaggagaa acacagccat gttactggca ccgtcacgat ctaaatatcg
     1021 caagcagcat cgcggtcgca tgaaaggaac cgcccagcgc ggaaacagcg ttgcctttgg
     1081 cgaattcgcc ctccaggcaa tggagccatg ctggatgaca gcccggcaga tcgagtccgc
     1141 tcgtatcgcg ctgacccgct tcatgaagcg tggtggcaag gtatggattc gcgtctttcc
     1201 agataagccg gtcaccgcca aacccgccga aacccgcatg ggtagcggca aaggtgcggt
     1261 cgatcactat gttgctgtga ttaagccagg ccgcatcatc ttcgagatcg gcggggtctc
     1321 tgaagaaatc gcgaaagagg cagcgcgcct ggccagtcat aagctgccga ttaagacccg
     1381 ctttatcgcg aaaggggagg ctgaaagtgc cgaagcttaa agaacgtcgc aagcaaatcg
     1441 ccgaaatgac gatcggcgag gcccagcgcg aactcaaaga tctgcgcgag aagctgttta
     1501 accttcgctt gcaacagcag cgcggcgaga tcaagaataa tcgcatcttt acgcagacga
     1561 aaaaagatgt cgcgcgtgtg ctgttccgtt tgaagcagtt ggagactgaa gaatgacaca
     1621 gcacgtaagg caatcgaaag cgcaggatgg gcaaaatgcc gtcaaggtcc gccaccagca
     1681 gaaggtaggc gtggtcatta gcaataaaat gcaaaaaacc attgtcgttt ctgtggagtc
     1741 gttgaagaag catcgcatct acaaacgtac ctataagcag accaagcatt ttcacgcgca
     1801 tgacgaaaca aatacctgtc agattggtga catggtacgc attgaggaga ctcgtccgct
     1861 cagcaaaacc aagcgctggc ggttagtcga gatcattaaa cgcagtagcg gggtcgtacc
     1921 ggtagatgaa ttgttggcca aggtggaccc tgacctgacc tcaggtgaag aggacgaatc
     1981 ctcggacgcc gaggaagcat aaggagggtt caatgatcca gccacagaca cgcctgaagg
     2041 tagccgataa tacaggcgcg aaggaaatta tgtgtatccg cgtgatgagc ggctcggcca
     2101 aacggtacgc gggaattggg gatgtcatca aagcctcggt caaggtggct gcccccaatg
     2161 gccaggtcaa gaagggtgaa gtggttgacg cggtaattgt gcgcgtgtcc aaagaatatg
     2221 cgcgtcccga cggctcgcac attcgtttcg atgagaacgc ggcggtcatc attgtcgccg
     2281 gtaataaccc gcgcggcacc cgtatctttg gaccggtggc ccgcgaattg cgcgatcgga
     2341 actttatgaa gatcatctcg ctcgcgcctg aagtgttgta aggacggaac aatcgtccga
     2401 tgtggttgtt ttaaggtttg tgttgaagtt tgtcgttgtg cgagtgcggc aaagacaaac
     2461 gcaaacaata gaggaaacaa atggcagtaa aacagaaagt gaaaaagcca cttcatctcg
     2521 ccaagatgaa tatcaagaag ggggataccg tcttgattat caccggcaaa gatcgaggga
     2581 agcaaggcac ggtatcgcgg gcgttaccac aggtcagcaa agtaattgtt gagggcctga
     2641 acatcgcgaa gaagcacgtt cgcccgcagg gccagactcg ccaggggggt atcattgaga
     2701 aggcgatgcc gctccatgtc tcgaacacga tgctgatttg caccgaatgc ggtgagccga
     2761 cgcgcgtggc gcatgatcgc cgcccgatgg gtgtggatca gaaggtgcgc ctggtgcgtg
     2821 tctgcaagaa atgtcataag atcatcgaag atcgcacgag gagtagttaa acatggcatc
     2881 cagactgaaa gagaaatacc agaaagaggt cgtgccagcc ctgcacaagg agtttaacta
     2941 caagaacccg atgcaggttc cgaccgtcaa caaggttgtg gtgaacattg gcatgggcga
     3001 ggcgatccag aacgcaaagg cgatggacgc ggcggtctcc gacctggcta cgatcaccgg
     3061 ccagcgcccg gtgattacgc gcgcgaaaca ttcggttgcg gccttcaagt tgcgcgaagg
     3121 gatgcagatc ggttgcatgg tgacactgcg cggcgagcgt atgtatcact tcctggataa
     3181 gctgatgaac gtggctctgc cgcgcctgcg cgacttccag ggggtttcag ccgatgcctt
     3241 tgatgggcgc ggcaactata ccctgggcct gcgcgaacaa ctggtcttcc ccgaaatcga
     3301 ctacgacaag atcgataaag tgcgcgggat ggaagtaagt attgtgacga cagctcgcac
     3361 cgatgaagag gggcgacagc tgctcaagtt gatgggcatg cccttcaaga aataaggcgg
     3421 agagtagtat ggcaaa
//
LOCUS       DASZAK010000002         4273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_674398, whole genome shotgun sequence.
ACCESSION   DASZAK010000002 DASZAK010000000
VERSION     DASZAK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4273
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674398"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VGT44_00040"
     CDS             <1..777
                     /locus_tag="VGT44_00040"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HEV2579210.1"
                     /translation="NDVKEKLKRGEPALGAWLSFPSVATARVMARLGFDWLVVDMEHS
                     AQSPALMADMVATIADAGTCAPIVRVPYNSVEWFKWALDAGAWGVVVPMVNSREEAQR
                     AIEFSKYPPLGNRSIGGIFGPYGFGTTDWPGYAQVANDEIMMIMQIESAQALQHLDEI
                     LSVPGVDVAFVGPNDLHAQLGLIPSSDGTEPAFVEALERVKVAAMKHHVALGIFSSDG
                     KAAVARVRQGFQMISVTTDVGSMIAAAAQNLRVAREERYD"
     gene            948..1436
                     /locus_tag="VGT44_00045"
     CDS             948..1436
                     /locus_tag="VGT44_00045"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV2579211.1"
                     /translation="MSVEHTQSQSTSRSVKVGDRAPDFTLPSQTGESVSLANFAGKNI
                     VLYFYPKDLTPGCTAEACAFRDSYEVFQEAGAEVIGISSDSEKSHQQFATQHRLPFIL
                     LSDPGSVVRKFYGVPTTFGLLPGRVTYIIDKQGVVRHIFSSQFAPQKHVEEALKVLRT
                     LT"
     gene            1624..3579
                     /locus_tag="VGT44_00050"
     CDS             1624..3579
                     /locus_tag="VGT44_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579212.1"
                     /translation="MTSIASAAVSIQTSPQSVPSTPSWFGEVAVVAHVLTRLGLLESI
                     GTAVRFARRRFGQYEVLDFVAVLIGYALSGEPTLHTFYERLQPFSTPFMALFGRTQLP
                     HRSTLSRFLAAIDPPTVEALRTLFLQDLLARPLEGAQSAGVWDRTGAQWLVLDVDGTR
                     QAARQRALPHTSDLPAASRRFEQVCAAGYQGRKRGEVVRTRTTILHAHTQQWMGTFSG
                     TGNGDYRGELLRAAEVIAHAVAQQHLPVDHAIVRLDGQYGNGTVVADLARLGLGWITR
                     GKDYALLDLPQVQARLARPPDQQTTHPETGTCRALFDCPEVLLTPTGPRSRVIIATHP
                     ATATPSPVGVTRDGVVYELFFTALPPGAFTPADVVALYLHRGAFELVLADEDQEQDPD
                     RWCSHRPCGQEFWQILNQWMWNLRLELGHGLHPTPMRMTEFAPAQTQPLAPPASHEPV
                     GLTYGPLAWARPAAMRGFAADAFTPQADGTLRCPAAQPLYVRERRPQRDGSLRVVYAA
                     RVGQCRGCPLREQCQGYGTATQQPRRVSAVLRPIAGPPLPPAAPPARAAPHPILWGDW
                     SRVQTRREWITLLRTQTVTLTLAAVAARATPTAGGPFSRRQRAHWRLSWEERLARNAC
                     CALVPAVALHLFGIPTTLATSLGLTSR"
BASE COUNT          872 a         1250 c         1242 g          909 t
ORIGIN      
        1 aacgatgtaa aggaaaaact gaaacgtggt gagccagcgc tgggcgcgtg gttaagcttt
       61 cccagcgttg ctacggcgcg agtgatggca cgacttggct ttgattggct tgtagtagat
      121 atggaacaca gcgcgcagag cccagccctc atggctgata tggttgctac tatcgccgat
      181 gcaggaacct gcgctcccat cgtgcgcgtt ccctataata gcgttgagtg gttcaaatgg
      241 gcgttggatg ccggggcatg gggggtggtt gtccctatgg tcaattcccg cgaagaagca
      301 caacgcgcaa tagagttctc gaaatatcct cctcttggca accgtagtat cggcgggatt
      361 ttcggtccgt acggattcgg cacaactgac tggcctggtt atgcgcaagt tgcgaacgac
      421 gaaatcatga tgattatgca gattgagagc gcgcaggccc ttcagcatct cgatgagatc
      481 ctctcggtgc caggtgtaga cgtggcattc gtggggccaa atgatcttca tgctcaactc
      541 ggcctcatac cgagcagtga cggcactgaa ccagcatttg tcgaagcgct ggaacgagtg
      601 aaagtggcag ccatgaagca ccatgtcgcc ctcggtattt ttagtagcga cggaaaggcc
      661 gcggttgcgc gagtgcgtca gggtttccag atgatcagtg tcactaccga cgtcggtagc
      721 atgatcgccg cggcagccca aaatttacgc gtagcgcgag aggaacgcta cgattgacag
      781 tgccgtttca caattctcat gtacgctcgc aaagctctct ttttagcttg cctgtttgtc
      841 atgtacgtgt aaccctgtgc gaatatatca ttagaaggtt gctgcataca ggatgtgaag
      901 tgcttctcgc acttaaccta gaacaaagga tttgaaggac ctataccatg agcgtcgaac
      961 atactcagtc tcaatcaacg agtcgcagcg tgaaggtcgg ggatcgtgct cccgacttta
     1021 cacttccctc ccaaacaggc gaatcagtga gtctggcgaa ttttgctggc aaaaatattg
     1081 tgctctattt ctaccccaag gatctgacgc caggctgcac tgctgaagca tgtgctttcc
     1141 gggatagtta cgaagtcttt caggaggctg gtgcggaagt tataggcatc agttcggact
     1201 ctgaaaagtc gcaccaacag tttgctacac agcaccgtct gcctttcatc ctattgagcg
     1261 atccaggcag tgtagtgaga aagttctatg gcgttccgac gacgtttgga ttactgccgg
     1321 gccgtgtaac ctacattatc gacaaacagg gggttgtccg gcatatcttc tcttcacaat
     1381 tcgcgccaca gaaacatgtc gaggaagcgc tcaaggtgct gcgaacattg acctagtgca
     1441 tactcaagag accattgcga gaatgggtga catcaccatg atggttcttc ctttggtatg
     1501 cacgagctga cccagtgaaa caactgacac aacccacgag atgcgcaacg aggtggtaaa
     1561 gagggcagaa atgtggcctc cttagtcagg aagactacgt gtatttccta cgaaggagga
     1621 cttatgacta gtatcgcatc tgcggcggtg agcatccaga cctcaccgca atcggttccg
     1681 tcgacgccgt cgtggttcgg cgaggtagcg gtggtggctc acgttctcac acgcctgggg
     1741 ctgctcgaga gcattggaac agcagtacgc tttgcccgcc gtcgctttgg ccaatatgag
     1801 gtgctggact ttgtcgccgt gctcatcggc tatgcgctca gcggagaacc gacccttcac
     1861 accttctatg agcgtctgca gcctttctcg accccgttca tggcgctgtt cggacgaacc
     1921 caactgcccc accgctcaac actcagtcgc tttctcgccg ccattgaccc gcccacggtc
     1981 gaggcactgc gcaccctctt cctgcaggat ctgctggcgc ggccattgga aggcgcacag
     2041 agcgcagggg tgtgggatcg gactggagcg cagtggctgg ttctcgatgt ggatgggacg
     2101 cgacaggcgg cccgacagcg agcgttacca cacacctcgg acctgcctgc tgcctctcga
     2161 cgcttcgagc aggtctgcgc tgcgggctat caggggcgca aacgtgggga ggtcgtccgg
     2221 acgcgcacca cgatcctgca cgcgcatacc cagcagtgga tgggaacctt ctctggcaca
     2281 ggcaacgggg actatcgcgg ggaactgctc cgagccgccg aggtcattgc tcacgctgtg
     2341 gcccagcagc acctgcccgt ggatcacgcc atcgtgcgac tggatggcca atatggcaat
     2401 gggacggtgg tggctgatct agcacgtctg ggactcggct ggataacgcg tggcaaggac
     2461 tatgcgctgc tcgatctccc ccaggtgcag gcgcgcctgg cccggccacc tgaccagcag
     2521 accacgcatc ccgaaacggg aacgtgccgt gcgctcttcg attgccctga ggtgctgctc
     2581 acgccgacgg ggcctcgcag cagggtcatc attgccacgc atccagccac ggccaccccc
     2641 agtccagtcg gcgtcacgcg agacggggtg gtctacgaac tgttcttcac cgccttgcca
     2701 ccaggcgcct tcacccctgc ggatgtggtt gcactgtacc tgcaccgggg cgcattcgag
     2761 ctggtgctgg ccgatgagga ccaggaacag gatccggatc gctggtgttc ccatcgcccc
     2821 tgcgggcagg agttctggca aatcctcaac caatggatgt ggaacctgcg cctcgagttg
     2881 ggccatggtc tccatccgac cccgatgcgc atgaccgaat ttgctcctgc ccagacgcag
     2941 cccctggccc cacctgcttc ccacgaaccc gttggcctga cctacggacc gctcgcgtgg
     3001 gcccggccag ctgcgatgag aggctttgcc gcagacgcct tcacgcccca agccgatggc
     3061 acgctgcgct gtcccgctgc tcagcccctc tacgtgcgag aacgacggcc ccaacgcgat
     3121 ggcagcctgc gcgtcgtgta tgctgctcgg gtgggccagt gtcgtggctg cccgctccgg
     3181 gagcagtgtc agggctatgg aaccgccacc cagcagccgc gccgtgtcag cgccgtgctg
     3241 cggcccatcg caggaccacc gctgcctcct gctgccccac ctgcccgtgc ggctcctcat
     3301 ccgatcctgt ggggagactg gagccgagtc cagacccgac gcgagtggat cacgttgctg
     3361 cgcacccaaa ccgtcacgct caccctcgct gcggtcgctg cgcgtgctac ccccaccgca
     3421 ggcggcccgt tctcgcgacg acaacgggcg cattggcgcc tgtcgtggga agagcgactg
     3481 gcacgcaatg cctgctgcgc gcttgtgcct gcggtagcgc ttcacctgtt cggcattccc
     3541 accaccttgg caacctcgct cggcttgacg agtcggtaaa ggtagatgac cggaacctcg
     3601 gcgctccggt ggccgagtcg tctcgtcatg ccctttgcac ccgcagatgg aggcgctgtg
     3661 ctgccttgac catcggtctc gtcgcgttcc accagtgcgg gccatgcctc tggctcaggc
     3721 tctggtcacg gaggagacga tgaacctgct ggtgcacgag cagcagaacg cccatgctct
     3781 tcctgaaacg cggtcgctct gtttgcagtg agatcctgtc tgtctcttcg tggcttgttt
     3841 cttcacctgg tcatctcgtt gcgcatctcg tgggactatc tgcaacaagt gcaacaaacg
     3901 caacaagtga tacaagtgat acaaatacag cttatacctt gccagagaag agtagacgta
     3961 tatgggctgt atgtacaagc aacccattac gcatgtcctg acaggtagag atatcgatct
     4021 gaaattgcct gacgcgatga acggattgtg acgcgaatga gatatcagag ctaacagccg
     4081 tgatacagat gtgaggggtg catgataatg tctaattgtg cgtgaaatgg ggccgggtgg
     4141 caaatcacct aaatccgtgt gtgggccacc cgagtacggg ggacatttcc gcacaatgcc
     4201 ctaccatttg aaacccatgt actacttcct gcgtattaag gtaaggttat acaaaggagg
     4261 tatagttgtg aat
//
LOCUS       DASZAK010000003         1674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_72, whole genome shotgun sequence.
ACCESSION   DASZAK010000003 DASZAK010000000
VERSION     DASZAK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..>1674)
                     /locus_tag="VGT44_00055"
     CDS             complement(32..>1674)
                     /locus_tag="VGT44_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2579213.1"
                     /translation="VGDGGVATNDFNALFRQASVHKLPVLMVVEDNGWAITTPSPIQW
                     SGSLVEWAKGAGVYAEEVDGTDTMATYEASVRLVEHVRSCQGPVLMHLRLGLLDPHSS
                     STDIRAYRTKEEIEATTATKDPVKNFGRWLIEHGHLQEGDLERIRKEVRAELERAEAQ
                     VLQEPEPSPERVLQNVLHVPHWQENTPRGTKKQLPMLGAINEALVELAQRDPGFFVYG
                     QDVGSPKGGVFGATSSLVTQFPGRAISSPLNEQVIVGIAAGAGMVDRKARCAEIQFVD
                     YHQSAAQTIRMAARTSYQSNGDWTVPLLIRTKSGSGGGGPISSGGAGGGAFGHSNAGE
                     QWFTSVPGMITICPATPFDAKGLLLEAARAQSPVAFLERGRLYRSEPPKDNQGNSIAA
                     MAEYWNVPEGYYTLPIGKARRVRIGNGPTSIAIISWGTMVLESCTAAANRVNRNGGAI
                     EVVDLRTLAPFDEEAVAAAVHEANRVIVVTEEYDLTSFGRHLHSWIVQNCFYDLDCTP
                     AFISALPAPAAPYNGPEETAFFPTSKTIEEKIEELLLE"
BASE COUNT          334 a          497 c          472 g          371 t
ORIGIN      
        1 tttagaagta ccgttatgag atcaggctat tttattccag cagcagctcc tcaatcttct
       61 cttcgatggt ctttgatgtc gggaagaatg ccgtctcttc tggaccattg taaggtgcag
      121 cgggagccgg cagcgccgag atgaaggcgg gtgtgcagtc gaggtcgtag aagcagttct
      181 gcacaatcca tgaatgcaag tggcgtccaa aactcgtcag gtcgtattcc tctgttacaa
      241 cgatcacgcg attggcctcg tgcacggcag cggctacagc ctcctcgtca aacggggcga
      301 gcgtgcgcag atcgaccact tcgatagcgc cgccattgcg gttgacacga ttggcggcag
      361 ccgtacacga ctccagcacc atcgtacccc agctaataat agcgatgctg gttggcccat
      421 tgccgatacg cacgcgtctg gccttgccta tgggcagcgt gtaatatcct tcggggacgt
      481 tccagtactc tgccatagcc gctatactgt tgccttgatt gtctttggga ggctcggagc
      541 gatagagacg cccgcgctcc aggaacgcta cgggtgattg cgcgcgtgcc gcttcgagca
      601 gcagtccctt ggcatcaaaa ggggtagccg ggcaaatcgt aatcatcccg ggcacgctgg
      661 tgaaccactg ctcgcccgcg ttggagtggc caaaagcgcc ccctccggcg ccaccgcttg
      721 agatggggcc accgccaccg ctgccggact tcgtgcggat aagcagggga acagtccaat
      781 cgccgttgct ctggtacgag gtacgggcag ccatacgtat ggtctgcgcc gctgactggt
      841 gatagtcaac aaactggatt tccgcacagc gtgcctttct atcgaccata cccgcacctg
      901 cagcgatacc cacgatgacc tgttcgttga gtgggctgct aatggctcgc cctgggaatt
      961 gcgtgaccag cgaagaggtc gcgccgaata ctccgccttt ggggctacct acatcctgcc
     1021 cgtagacgaa gaagccggga tcacgctgcg ccagttctac cagcgcctcg ttgattgctc
     1081 ccagcatggg caattgcttt tttgtgccgc gcggggtatt ctcctgccag tgtggtacat
     1141 gcaatacatt ctgcaggaca cgttccggcg aaggctcagg ctcctgtaag acctgggcct
     1201 cggcgcgttc caattcggcc cggacttcct tacggatccg ctctaaatcg ccttcctgca
     1261 ggtgaccatg ttcgataagc cagcgaccaa agtttttcac cgggtctttg gtggccgttg
     1321 ttgcttcgat ctcttccttg gtgcggtatg ctctgatatc ggtgctgctg gaatgtggat
     1381 cgagcaggcc caggcgcaaa tgcatcaaga cagggccttg acacgagcgc acgtgctcaa
     1441 cgaggcgcac actggcctca taagttgcca tagtatctgt accatcaacc tcttcggcgt
     1501 agacgcctgc gcctttagcc cattcaacga gcgatccact ccattggatg ggggaaggag
     1561 tagtaatagc ccacccattg tcctcaacca ccatcagcac gggcaacttg tgcaccgatg
     1621 cctggcgaaa cagggcgttg aaatcgttgg tcgccacccc accgtcgcct acag
//
LOCUS       DASZAK010000004         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_674440, whole genome shotgun sequence.
ACCESSION   DASZAK010000004 DASZAK010000000
VERSION     DASZAK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674440"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VGT44_00060"
     CDS             complement(<1..456)
                     /locus_tag="VGT44_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV2579214.1"
                     /translation="MAKKKFERTKPHVNVGTIGHIDHGKTTLTAAITKVLAKANASNK
                     YIAFDQIDKAPEERERGITIAIAHVEYETEKRHYAHVDCPGHADYIKNMITGAAQMDG
                     AILVVSAPDGPMPQTREHVLLARQVEVPAMVVFLNKVDMMDDEELLELVE"
     gene            complement(613..2688)
                     /gene="fusA"
                     /locus_tag="VGT44_00065"
     CDS             complement(613..2688)
                     /gene="fusA"
                     /locus_tag="VGT44_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV2579215.1"
                     /translation="MAREYPLEKTRNIGIIAHIDAGKTTTTERVLFYTKKIHRMGEVH
                     EGAATMDWMPQEQERGITITSAATTCFWLDHRINIIDTPGHVDFTAEVERSLRVLDGG
                     VVVFDAVAGVEPQSETVWRQANKYNVPRICFVNKMDRTGADFWRTVEMIRERLGAVPV
                     PIQIPVGQENNFKGFIDLVEHQAVIFTDDLGTKSDHTQVPTGMEAEFEQHREFLIERV
                     AETDEELTLKYLEGEEITKEEIIAALRKATIAGRLVPVLCGSALRNKGVQAMLDAVIA
                     YLPSPLDIPLPNGIDPNSGEAITRDVSDNAPFSALVFKIVSDPFVGRLSYLRVYSGMI
                     AKGASVENSTKDKTERFGRLLRMHANHREDIEEIRAGDICAAVGLRNTFTGDTLCDAS
                     HPIVLEAITFPQPVISIAIEPRTRADQDKMAIALNKLAEEDPTFQLRTDVESGQTIIS
                     GMGELHLEVIVDRLFREFKVEANVGRPQVAYRETITKDAQAQGKHVRQTGGHGQYGDV
                     WIRVTPNERGKGFEFVNAIVGGSVPKEYIKPVENGIRETLENGILAGYPMIDVKVTLF
                     DGSYHEVDSNEMAFKTAGSLAIKEAVRKAGPILLEPIMLVEITTSEEFYGDVIGDLNR
                     RRGSIMGMDNRGTMVVVRGHVPLSEMFGYVNDLRSMTSGRANYSMEFSHYDPVPKGIG
                     ETIIAKATR"
     gene            complement(2701..3171)
                     /gene="rpsG"
                     /locus_tag="VGT44_00070"
     CDS             complement(2701..3171)
                     /gene="rpsG"
                     /locus_tag="VGT44_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV2579216.1"
                     /translation="MPRRKRAVRRPDVPDAKYKSRNVNRFIGKLMLDGKRSLAERIIY
                     TSFELIEARQKRAPLDVFEQALKNATPTLEVKPRRVGGSTYQVPVDIRRERGNALAMR
                     WLIRSARTRTGKSMSEKLAAELMDAAAGQGATIKKREETHKMAESNKAFAHFRW"
     gene            complement(3516..3968)
                     /gene="rpsL"
                     /locus_tag="VGT44_00075"
     CDS             complement(3516..3968)
                     /gene="rpsL"
                     /locus_tag="VGT44_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV2579217.1"
                     /translation="MPTINQLIRKGRSQKKQKVKAPALRYSFNALKNKTVSLRGSPQK
                     RGVCTQVRTMTPKKPNSAMRKIARVRLSNRMEVTAYIPGEGHNLQEHSVVLIRGGRVK
                     DLPSVRYHIVRGTLDSDGVSKRRRGRSLYGAKRPKPGQAAAPAKGGKK"
     gene            complement(4095..>5045)
                     /locus_tag="VGT44_00080"
     CDS             complement(4095..>5045)
                     /locus_tag="VGT44_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV2579218.1"
                     /translation="VEVVKDEATGVRTVRVVSTNVFFDEYHLPQGSQVLVADQAHVQK
                     DEVIALMPAEEGSQPVPVMARTEGEALLNADGLLSIRFEEREERAYQIPAAKSIAVNT
                     GQKIQAGTAITAGQRDPQDVLRIQGREAVQMYLVKEVQRVYRNTGVYINDKHIEVIIR
                     QMLRRVRVEEPGDTDMLPNDLVDRFIYADTNARVLAEGGEPATAQTVLLGVTKASLNT
                     DSFLAAASFQETTRVLTEAAIEGQTDHLVGLKENVIIGKLIPAGSGFVQRRRELLARQ
                     QSAANRLANAQAAQAVASFGATVPGSVGTLEAVLDSFTDE"
BASE COUNT          945 a         1460 c         1446 g         1194 t
ORIGIN      
        1 ctccaccaac tctaagagtt cctcgtcgtc catcatgtcg actttgttga ggaagacgac
       61 catggccggc acttccacct gccgagccaa caagacgtgc tcgcgcgtct gaggcatggg
      121 gccatccggg gcgctgacca ccaggatggc cccatccatc tgggcggcgc cggtgatcat
      181 attcttgata tagtcggcgt ggccggggca gtccacgtgg gcgtagtgcc gcttctcggt
      241 ctcgtactcc acgtgggcga tggcgatggt gatgccgcgc tcgcgctcct cgggcgcctt
      301 gtcgatctgg tcgaaggcga tatacttgtt ggaggcgttg gctttggcca gcactttggt
      361 gatggccgcc gtcaaggtcg ttttgccatg atcgatgtgt ccgatcgtgc ccacgttcac
      421 gtggggcttc gttcgctcaa atttcttctt tgccattttt tcctctcgcg ctctgtgcat
      481 acgtgcgtca ggggttgcac ctttgcgatg gcgctcaatc cttgttattt cgtttttgta
      541 aacaccgatt tgtacagata cgattattac ccggtaggcc catcccctga tgcgggcgct
      601 gggtgttcaa cgttacctcg tggctttggc tataatcgtt tcgccaatgc cctttggtac
      661 tggatcgtaa tgcgaaaact ccattgagta gttggctcgc ccgctggtca tggagcggag
      721 gtcattgaca tagccgaaca tttcggacag cgggacgtgg ccccgcacta cgaccattgt
      781 tccccggtta tccattccca tgatgcttcc acgacgccgg ttcaagtcac cgatgacatc
      841 gccgtagaac tcttcggagg tggtgatctc tacgagcatg attggctcaa gcaggatcgg
      901 gcctgctttg cgcacggcct ctttgatcgc cagcgaaccg gcggtcttaa aggccatttc
      961 attcgagtca acttcgtggt aagaaccgtc gaagagtgtg accttgacat cgatcatagg
     1021 atacccggcc aggatgccgt tctccaatgt ctcgcgaatg ccgttctcga cgggtttgat
     1081 gtattctttt ggcactgagc cgccaacaat ggcgttgacg aactcaaagc ctttaccgcg
     1141 ttcgttcggg gtgacgcgaa tccacacgtc accgtactgc ccatgaccgc ctgtttgccg
     1201 gacgtgcttg ccttgcgcct gcgcatcctt cgtgatggtc tcgcgatagg cgacctgggg
     1261 gcgtccaacg ttggcctcaa ccttaaattc gcggaagagg cggtctacta tgacttccag
     1321 atgtaactca cccatgccgc tgatgatggt ctggccactc tcgacatcgg tgcgtaactg
     1381 gaaggtcggg tcttcttctg ccagtttatt cagggctatc gccatcttgt cctggtcggc
     1441 gcgtgtcctc ggctcgatgg caatcgagat gaccggttgc gggaacgtga tcgcttcaag
     1501 cacaatcggg tggctggcat cacacaatgt atcaccggtg aaggtgttgc gcagcccgac
     1561 ggcggcgcaa atgtcgccgg cgcgaatctc ttcgatatcc tcgcggtgat tggcgtgcat
     1621 acgcagcagg cgaccaaaac gctcggtttt atcttttgta ctgttttcga ccgaagcgcc
     1681 tttcgcaatc ataccggaat aaacgcgcag gtacgagagt cgcccgacga acggatcgga
     1741 gacaatcttg aagacgagcg cgctgaaagg ggcattgtcg ctgacatcac gggtgatggc
     1801 ttcgccgcta ttgggatcga tgccgttggg aagaggaatg tcgagtgggg agggaaggta
     1861 ggcaatcact gcgtccaaca tagcctgtac gcctttattg cgcagggctg agccgcacag
     1921 caccggcaca agtctcccgg cgatggtggc tttgcggaga gctgcgataa tctcctcttt
     1981 ggtaatctct tcaccctcaa ggtatttgag ggtcaattcc tcgtccgttt cggcgacacg
     2041 ctcgatcaga aattcgcggt gctgctcgaa ctcagcttcc ataccggtcg gcacctgggt
     2101 atggtcggat ttggttccca ggtcatcggt gaatataacc gcctggtgtt cgacaagatc
     2161 gataaagcct ttgaaattgt tctcctggcc gacaggaatt tgaattggga ccggcaccgc
     2221 gccgagtcgc tcgcgaatca tctcgaccgt gcgccagaaa tcagcgccgg tgcgatccat
     2281 tttattgaca aaacagatgc gtgggacatt gtatttgttg gcctgtcgcc agaccgtttc
     2341 cgactgcggt tcgacaccgg ctacagcatc gaagacgact acgccgccgt cgaggacgcg
     2401 caaagagcgc tcgacctcgg cggtaaaatc gacgtggccg ggcgtatcga tgatattgat
     2461 gcggtgatcg agccagaaac acgtcgtagc ggcagacgta atcgtgatac cgcgctcctg
     2521 ctcctggggc atccagtcca tggttgcggc cccttcatgt acctcaccca tgcggtggat
     2581 ttttttggta tagaaaagga cacgctcggt cgtggtggtt ttcccggcgt caatatgagc
     2641 aataattccg atatttcgtg ttttttccag tgggtattct cgtgccatga ttcctgtatg
     2701 ttaccagcga aaatgagcaa aggccttgtt ggactccgcc attttatggg tttcctctcg
     2761 tttcttaatg gttgcgccct gaccggcggc ggcatccatc aactcagccg ccagcttttc
     2821 ggacatcgac ttgccggtgc gagtgcgggc ggagcgaatc agccagcgca ttgcgagggc
     2881 attgccgcgc tcccggcgaa tatcgacggg aacctggtag gtcgagccac cgacgcggcg
     2941 gggtttgact tccagggtgg gtgtggcatt tttcagcgcc tgctcaaata catccagcgg
     3001 cgcacgcttc tgacgcgctt cgatcaactc gaaactagta taaatgatgc gctcggccag
     3061 gctgcgcttg ccatcgagca tgagctttcc aataaagcga ttgacgttgc gactcttata
     3121 tttcgcatcc ggcacatccg gtcgccgtac cgctcgtttt cttcttggca taactgcctc
     3181 ctcgttatcc gatcttctgc ctgtttcgac ctacacccgg cccaggccag atgcattttt
     3241 aaacatcaca aaaggcgtcg attcgatggc aggcgcatac ctgccgtcgt cttctgacgc
     3301 ctttgcgcga gcgcctatcc tcaatgctcc gtgcgaccag agagccgcca ctacatgtac
     3361 ccgtaaacgg gccatgcctg aaaaggagga cacccgacta tcgctgggga aggtaacaat
     3421 caacaatccc tgacgattca cgatttgtcg acctacctga aggcgttcag gcggtatcgt
     3481 ttcagcatgt tacacggacg cgcataggcg cgtctctact ttttgccgcc tttggcgggg
     3541 gcagctgcct gcccaggttt tggccgcttg gcgccataga gcgagcgacc acgacggcgc
     3601 ttggagacgc catcgctgtc cagcgtgcca cgcacaatgt ggtaacgcac acttggcagg
     3661 tccttcacac gtccgccgcg aatgaggacg acggagtgtt cctgcaaatt atgtccttca
     3721 ccggggatgt aggcggtcac ttccatccgg ttgctgagcc gcacgcgagc aattttgcgc
     3781 attgccgagt ttggcttctt gggggtcatc gtacgcacct gggtgcagac gccacgcttc
     3841 tgcggggacc cacgcaagct cactgttttg ttcttcagcg cattaaacga atatcgcagc
     3901 gctggggcct tgaccttctg ctttttctgc gagcggccct tgcgaatcaa ctgattaatc
     3961 gttggcaatg aacacattct ccctgttacg acgttatcgc tttcgtagag gacgcgatat
     4021 atcgccctcc atgagtattg cggtagaggg cgcgacgtat cgcgccccta cgagtattgc
     4081 ggtttcttat ggtatcactc atcggtgaaa ctgtcaagta ccgcttcaag agtaccaaca
     4141 ctgcccggca cggtcgcgcc aaagctggca acggcctgcg ctgcttgcgc gttagcaaga
     4201 cggttcgctg ccgactgctg gcgcgccagc agctcgcggc ggcgctggac gaaaccggag
     4261 ccagccggaa tgagcttgcc gatgatgaca ttctccttca agcccaccag gtgatcggtc
     4321 tggccctcga tggcggcctc cgtcaacacg cgcgtcgtct cctggaagct ggcggcggcc
     4381 aggaagctgt cggtgttgag cgacgccttg gtgacgccca gcagcacggt ctgcgccgtg
     4441 gcaggctcgc cgccctcggc cagtacgcgg gcgttcgtgt ccgcgtaaat gaagcggtcg
     4501 accaggtcgt ttggcaacat gtcggtatcg cccggttcct caacgcgcac gcggcgcagc
     4561 atctggcgga taatcacctc gatatgctta tcgttgatat acacgccggt gttgcgatag
     4621 acacgctgta cctctttcac gagatacatc tgcacggctt cgcgaccctg gatgcgcagg
     4681 acatcctggg gatcgcgctg gcccgcggtg atcgctgtac cagcctggat tttctgaccg
     4741 gtattgacgg cgatgctttt cgcggcaggg atctgataag cgcgttcctc gcgctcctcg
     4801 aagcggatgc tcaaaaggcc atcggcgttg agcagcgcct cgccctccgt gcgagccatg
     4861 accggaaccg gctggctgcc ctcttcggcg ggcatgaggg cgatgacctc gtccttctgc
     4921 acgtgcgcct ggtcagcgac cagcacctgg ctgccttgcg gcaggtgata ctcgtcgaag
     4981 aagacgttgg ttgaaaccac gcgcacggtg cgcacgccgg tcgcctcgtc cttcacgact
     5041 tcgac
//
LOCUS       DASZAK010000005         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_35558, whole genome shotgun sequence.
ACCESSION   DASZAK010000005 DASZAK010000000
VERSION     DASZAK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35558"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VGT44_00085"
     CDS             complement(<1..937)
                     /locus_tag="VGT44_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEV2579219.1"
                     /translation="MKESSYRERDYAFGQLMLTLRTKLKLTQTALADLLAVRRRAVID
                     WEGGLTYPSVDHLKHLVVLAIERQAFPAGREAEEVRALWHTARQKVLLDEAWLGGLLS
                     HIQAPPASQLGEETSVTGHALASPSRGGPRVDWGDAPDVANFYGREWELDLLSEWVVE
                     ERCRVVSVLGQGGIGKSALATQVMHRVAEGFEVVIWRSLRDVPSCEALLDDCLQVLAP
                     QGLRSGSASLESRQSLLLDCLRSRRVLLVYDNLESFLQEGQDSGHMRPGSEGFARVLR
                     RVAETEHQSCWLLTSREKPSELVPLEGSRAPVRALR"
     gene            1151..1582
                     /locus_tag="VGT44_00090"
     CDS             1151..1582
                     /locus_tag="VGT44_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579220.1"
                     /translation="MIDIYAVAGTFADPKQLAIDAANTVKTIEGVPDIPMVRKNTAAF
                     VHELPAGTLANVDGDETYVRVQVLTNEGALDRNKQVAVVEQLTKLVSAAAGNETLQER
                     TWVLLTEAKEGGWGLWGHAHTNDELVAAARDEIAKLRSSSS"
BASE COUNT          486 a          601 c          616 g          385 t
ORIGIN      
        1 ggcgcaaggc acgcacggga gcccgactgc cttccagcgg cacgagctcg ctgggtttct
       61 cccggctggt cagcagccag caactctgat gctcggtctc ggccaccctg cgtagcaccc
      121 gggcgaatcc ctcggagccg ggacgcatgt gaccgctgtc ctgtccttcc tgcaggaacg
      181 actccaggtt gtcatagacc agcaggaccc gccggctgcg caagcagtcc agcaggaggc
      241 tctggcggct ttcgaggctg gcagagccgc tgcggagccc ctgtggagcc aggacctgga
      301 ggcagtcatc cagcagcgct tcgcagctgg ggacatcgcg cagggagcgc cagatgacca
      361 cctcgaagcc ctccgccacg cggtgcatca cctgtgtcgc cagggccgac ttgccaatgc
      421 ccccctggcc cagcacgctc accacccgac agcgctcttc gaccacccac tcagagagca
      481 ggtccagttc ccactcgcgc ccatagaagt tcgccacatc cggcgcgtcg ccccaatcga
      541 cgcgtggccc gcccctggaa ggagaagcga gagcgtggcc agtgacactg gtctcctctc
      601 cgagctggga tgccggggga gcctgtatgt gggacagcag cccaccaagc caggcttcat
      661 ccaggagcac cttctggcgg gccgtgtgcc agagcgcacg cacctcctct gcctcacggc
      721 cggcggggaa agcctggcgc tctatggcca ggaccacgag gtgtttgagg tgatcgacac
      781 tgggataggt gaggcctccc tcccaatcga tcacggcgcg gcgcctgacg gcaagcagat
      841 cagccaatgc ggtttgcgtc aacttgagct tcgtgcgcaa ggtcagcatc agctgcccaa
      901 aggcgtagtc tcgttcgcgg tacgaggact ccttcatgga tgtttccttt tcgtctcctt
      961 ctcacaagtg ctgcgatgtg aagcaatgtg cggtgcgaaa gtgtcgcgat gtccggtgac
     1021 ttcgagtacg ctccttagta tactacaaaa cagaagcctc ctcgcactga acggcttgga
     1081 cctattctga gggatcctgt ttgtctacac atcaactact catgctgaca gagaggggaa
     1141 cgctatgcca atgatcgata tctacgccgt tgctgggacg tttgccgatc cgaagcaact
     1201 ggcaattgat gcggccaata cagtcaaaac gatcgaggga gtgcctgata tcccgatggt
     1261 ccggaaaaac accgccgcat tcgtgcacga gttgccagca ggcacactcg ccaatgtgga
     1321 tggtgacgaa acctatgtac gcgtgcaggt acttacaaac gagggcgcct tagaccgtaa
     1381 caaacaggta gccgtggtag aacagctgac gaagctcgtg agtgctgctg ccggtaacga
     1441 aacacttcaa gaacgaacat gggtgctctt aacagaagcg aaagaagggg gctggggcct
     1501 ctggggccat gctcacacga atgatgaatt agtggccgcg gcacgcgatg aaatcgcgaa
     1561 gctgaggtct tcgtcaagtt aacatcggca tgggagagcg aaagaacaac agagcacctt
     1621 tttaggcatg tgttcacgaa ttaccaacgg gttcactgtc tcatggagac ggcgatgaaa
     1681 atgatgttcg tgtgcaggtt ttcaccaaca agggcgcgct agagagggac aagcaacgag
     1741 caggagttga gcaaatcaca aacgtagtag cttcagcagc ggatgacgaa agtcttcaag
     1801 aacgaacttg ggtacggctt ctcgaagctg aagaaagtga ttggggcttc tggggtcacg
     1861 ctcataccaa tgaagagctt gtgactgctg cccgcaatga aattgcaaag cgtaaaggcg
     1921 aggagttaag ttatggcaac gcaaagcaac gcatcaagct attcagcact tccgacacac
     1981 aaaatcacag caagtaacgg catcgagtat gcctatcgag aggtcggcac aagcccggtg
     2041 ccgcttgtgc tgctgcagca cttcagaggg aacctcgata actgggac
//
LOCUS       DASZAK010000006         1680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_390528, whole genome shotgun sequence.
ACCESSION   DASZAK010000006 DASZAK010000000
VERSION     DASZAK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390528"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VGT44_00095"
     CDS             <1..278
                     /locus_tag="VGT44_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579221.1"
                     /translation="RLIMISERLMKEETLEGTLFESLFNQPLEGEQYESPSILAGMPR
                     LNPSAHSDNGDSRSNGEYGQQPLLPEPGMSYPLPPQLQPPYSNMETP"
     gene            275..1219
                     /locus_tag="VGT44_00100"
     CDS             275..1219
                     /locus_tag="VGT44_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579222.1"
                     /translation="MIDFAPSFAAAFRKPGTETDAYAARIQEFTEAGRIARYLPAAGD
                     RERIALILVDCQHDFVEPSGALYVPGSQEDMARLLRWFYANGHKITTVYASLDTHIPF
                     QIFYSSWWRQPATGVHPPPFTVITAGDVEQNAWTPLSEPEWSAYYVHQLQQQAKKDLM
                     IWPYHTIRGTLGHMLVAPLSEAIAWHSSARATQPVYVEKGLTPRTEFYGIFGAEVADP
                     RAPESSLNTALIDTVMQHDRVYIAGEAKSHCVLETERQLVTYLADRPDMLQKLYLLKD
                     CASSVQHPTIDFDALAEAELARMQQMGVHLVTSDELIS"
     gene            complement(1267..>1680)
                     /locus_tag="VGT44_00105"
     CDS             complement(1267..>1680)
                     /locus_tag="VGT44_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579223.1"
                     /translation="LISQKDAQKTTEQRFEQTGDHLLARCAFEELDRVLLVVQLSPTT
                     HEDAYTAFFQRALQQGIIESYEYVTCYPVIAFLKPSPSRQEQMSSFPLRDGESWFPAI
                     DHPNKVYICLDKPLMASNQSAWLIRHQARWEYEEM"
BASE COUNT          377 a          502 c          464 g          337 t
ORIGIN      
        1 cgcgcctgat tatgattagc gagcgcttga tgaaagaaga aacgctcgag gggacactct
       61 tcgagtcgct cttcaaccag ccccttgagg gcgagcagta cgaatcgccc tccatcctgg
      121 ccgggatgcc gcgcctgaat ccatcggccc acagtgataa cggtgacagc cgaagcaatg
      181 gggaatatgg ccagcagccc ttgctgccgg aaccaggcat gtcttaccct ctccctcccc
      241 aattgcagcc gccgtattcc aatatggaaa cgccatgatt gatttcgcac catcttttgc
      301 cgctgcgttt cgcaaaccag gcacagaaac cgacgcctat gccgcgcgca tccaggagtt
      361 caccgaggcg ggccgcatcg cgcgctatct cccggcggcg ggcgatcgcg agaggatcgc
      421 gctgatcctg gtggattgcc agcacgattt cgtcgagcca tccggcgcgc tctacgtgcc
      481 aggatcgcag gaggatatgg cgcgcctgct gcgctggttc tacgccaacg ggcacaaaat
      541 taccaccgtc tatgcctcgc tggatacgca catacccttc cagattttct acagttcctg
      601 gtggcgacaa cccgctacag gcgtccatcc cccaccattc accgtgatta ccgccgggga
      661 tgtcgagcag aacgcatgga cgccgctttc agagccggaa tggtcggcgt actacgttca
      721 ccagttgcag cagcaggcaa agaaagatct gatgatctgg ccgtatcaca ccatacgggg
      781 aacgctgggg catatgctgg tcgcgccgct cagcgaggcc atcgcgtggc acagctcggc
      841 cagagcaacg cagccggtct atgtcgaaaa gggactgacg ccgcgcaccg agttttacgg
      901 catcttcggc gcggaggtag ccgatccgcg cgcgcccgaa agcagtctga acaccgcctt
      961 gatcgatacg gtgatgcaac acgaccgcgt ctacatcgcg ggcgaggcca aatcccactg
     1021 tgtgctggaa accgagcggc agcttgtcac ctacctcgcc gaccggccag acatgctgca
     1081 aaaactctac ctcctcaagg attgcgccag ctcggtacaa catcccacca tcgacttcga
     1141 cgcactggcg gaggcagagc tagcgaggat gcagcagatg ggcgtgcatc tcgtcacgag
     1201 cgacgaactg atctcctgat gggcagcgat ttctaaatgc cttaatgctt ccggtggaac
     1261 ctgaacctac atctcttcat actcccaccg cgcctgatgt ctgatcaacc atgcggattg
     1321 attggatgcc atcaacggtt tgtccagaca gatgtagacc ttgttcgggt gatcgatggc
     1381 tgggaaccac gactccccat ctcgcagcgg gaaactactc atttgctcct gcctgcttgg
     1441 agaaggtttc agaaaggcaa tcacaggata acaggtcaca tactcgtagg actcgataat
     1501 gccttgttgg agcgcacgtt ggaagaaagc agtatacgca tcttcgtgag tagtaggcga
     1561 aagctggaca acgaggagga ctcgatctaa ctcttcgaat gcacatcgag cgagtaggtg
     1621 gtcgcccgtt tgttcaaagc gttgctcagt agttttctga gcgtctttct gagagatcaa
//
LOCUS       DASZAK010000007         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_35598, whole genome shotgun sequence.
ACCESSION   DASZAK010000007 DASZAK010000000
VERSION     DASZAK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35598"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..2935)
                     /locus_tag="VGT44_00110"
     CDS             complement(125..2935)
                     /locus_tag="VGT44_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2579224.1"
                     /translation="MTEIPLKEHIAPSGEPMTVRDLASCPPPERWRDWMEYDAKAWPR
                     KVERHYEIIPTICFNCEAACGLLAYIDKESGRVKKLEGNPYHPGSRGRNCAKGPATIN
                     QVNDPERILYPLKRAGKRGEGKWERVTWDEALETFASKIRAALVEGRRDEVMYHVGRP
                     GHDGYMERVLGAWGVDGHNSHTNVCSSSARFGYHIWCGMDRPSPDHANARFILLISSH
                     LETGHYFNPQAQRIIEGKMKGAKLAVMDPRLSNTASMADYWLPTWPGSETAVLLAMAR
                     ILLAEGLYNRDFVRRWVNWQDFLAAEYPNVEQTFERFIEVLLDLYDEFTPAYAAQESG
                     LSEEVIIDIARQIGQAGTAFAAHTWRAATAGNLGGWQVSRALWFLSVLTGSIGTPGGT
                     APNAWNKFVPAPFLKPSPGEVWNELLFPPEYPLANYEMSILLPYFLREGRGKLAAYFT
                     RVYNPVWTNPDGTAWIEMLTNEQQIELHAALTPVWSETAWYADYILPMGVASERHDLM
                     SQETHAARWIGFRQPVLRVYQEHQGKSVQYTYQANPGEVWEEDEFWIELSWRIDPDGS
                     LGIRKYFESPYRPGEKLSILEYYRWMFENSVPGLPEVAQREQLTPLEYMQKYGAFEIP
                     QEQAPVFRLHERELTTADLEQAHEQDGLVKRNGATIGVMIEGKAYEGFPTPSRKLEFY
                     SATLKEWGWPEYSVPGVTTSHVHPSNIDHTRDEYLLIPTFRLPTLIHTRSGNAKWLYE
                     ISNANPVWIHPLDAERMGVQTGDLVRVTTEIGLYVNRAWVTEGIRPGIVACSHHLGRW
                     RLDTGSGVDRWASALVELGRAEDGAWRMRHREGVRPFESDDPDSARIFWREGGVHQNL
                     TFPVHPDPVSGMHCWHQKVTVERAREGDRYGDVFVDTAKAYEVYQAWLAMTRPQPDRT
                     DGLRRPLWMIRPYRPAASAFKR"
BASE COUNT          536 a          925 c          931 g          613 t
ORIGIN      
        1 aatactgcga cgagcgttcc acatgcatat tttagaaata acgaaaatat gcaattattg
       61 taaccgccat ttggggaaaa atcaagtggc caaagcaaac caggcaggcg gcaaaggcgc
      121 tggcttaccg cttgaaggcg ctggccgccg ggcgatacgg tcgaatcatc cacaacgggc
      181 ggcgcaggcc atcggtacga tcaggctgcg ggcgcgtcat cgccagccac gcttggtata
      241 cttcgtaagc cttcgccgta tccacgaaca catcgccgta gcggtctccc tcgcgcgccc
      301 gttccaccgt gaccttctgg tgccagcagt gcatgccgct gacgggatcg ggatggactg
      361 ggaaggtcag attctggtgg acgccgccct cgcgccagaa gatgcgcgcc gagtcagggt
      421 cgtcgctctc aaacgggcgc accccctcgc gatggcgcat ccgccaggcg ccgtcctccg
      481 ctcgtcccag ctcaaccagc gccgaggccc accgatcgac gccgctgccg gtgtccaatc
      541 gccagcgtcc gagatggtgc gaacaggcca cgatgccggg acgaataccc tcagtcaccc
      601 aggcgcggtt gacataaagg ccgatctcgg tcgttactcg taccaggtcg cccgtttgca
      661 ctcccatgcg ctctgcatca aggggatgaa tccagactgg attggcgttg gatatttcat
      721 atagccattt ggcattgccc gaacgggtat ggatcagcgt tggcaggcgg aaggtcggga
      781 tcagcaggta ctcgtcgcgc gtgtgatcga tgttcgaggg atggacgtgg ctggttgtga
      841 cacctggcac gctgtattcg ggccagcccc actctttgag cgtcgccgag tagaattcca
      901 gtttgcgcga gggcgtgggg aagccttcgt aagctttgcc ctcgatcatc acgccaatcg
      961 ttgcgccgtt gcgtttgacg aggccgtcct gctcgtgcgc ctgctccagg tctgcggtgg
     1021 ttagctcgcg ctcgtgcaga cggaagacgg gtgcttgttc ctgggggatc tcgaacgcgc
     1081 cgtacttctg catgtattcc aggggcgtca actgctcgcg ttgcgccacc tccggcaggc
     1141 caggaacgct gttctcgaac atccagcggt aatattcgag gatactcagt ttttcgccgg
     1201 ggcgataggg cgactcgaaa tatttgcgaa tgcccaggct gccatcgggg tcgatgcgcc
     1261 aggacagttc aatccagaac tcatcctcct cccacacttc gcccggattg gcctggtagg
     1321 tgtactgcac cgattttccc tgatgctcct ggtagacgcg cagcaccggc tggcgaaagc
     1381 cgatccagcg cgccgcgtgc gtctcctggc tcatcaggtc gtggcgttcg cttgccacgc
     1441 ccatcggcag gatgtagtcg gcgtaccagg ccgtctcgct ccacacgggc gtcagggctg
     1501 cgtggagttc gatttgctgc tcgttggtta gcatctcgat ccaggctgtg ccatcgggat
     1561 tggtccagac cgggttgtaa acgcgcgtga agtaggcggc cagcttgccc cgcccttcgc
     1621 gcaggaagta gggcaacagg atgctcattt cgtagttcgc caggggatac tccggcggga
     1681 agagcagttc gttccacacc tcgccggggg agggtttgag aaaaggcgcg ggaacgaact
     1741 tattccaggc gttgggggcg gttccgccgg gtgtgccgat gctgccggtc agcaccgaga
     1801 ggaaccacag ggcgcgcgag acctgccagc cgcccaggtt gcctgccgtt gccgcccgcc
     1861 aggtatgcgc ggcaaaagca gttcccgcct ggccgatctg ccgcgcgata tcgataatga
     1921 cctcttccga cagaccgctc tcttgggcgg cgtaggccgg ggtaaattca tcgtacagat
     1981 cgagcagcac ttcgatgaaa cgctcgaatg tctgctcaac gttgggatat tctgccgcga
     2041 gaaagtcctg ccagttgacc cagcgccgca cgaagtcgcg gttatacagt ccctcggcca
     2101 gcagaatgcg cgccatcgcc agcagcaccg ctgtttccga gccgggccag gtgggcagcc
     2161 agtaatcggc catgctcgcc gtgttggaga ggcgcgggtc catgacggcc agctttgctc
     2221 ccttcatctt gccctcgatg atgcgctgtg cctgcggatt gaaatagtgg ccggtttcca
     2281 ggtgcgagct gatgagcaaa ataaaacgcg cgttggcgtg gtcgggcgaa ggccggtcca
     2341 tgccgcacca gatgtggtag ccgaaacgcg ccgaggagga acacacattg gtatggctgt
     2401 tgtggccatc gacgccccag gctcccagca cgcgctccat atacccgtcg tggccaggtc
     2461 tgcccacgtg gtacatgacc tcatcgcgcc gcccttcgac gagcgcggcg cgtatctttg
     2521 acgcaaacgt ctccagcgcc tcgtcccacg tcacgcgctc ccatttgcct tcgccgcgct
     2581 tgcccgcgcg cttcaaagga tagaggatgc gttctgggtc gttgacctgg ttgatggtgg
     2641 ccggcccttt ggcgcagttg cgcccgcgac tgccgggatg gtaggggttg ccttccagct
     2701 tcttgacgcg cccgctctct ttatcgatat acgccagcag cccgcaggcc gcctcgcagt
     2761 tgaagcagat ggtggggatg atctcataat gccgctcgac tttgcgcggc cacgcctttg
     2821 cgtcatactc catccagtcg cgccagcgct ccggcggcgg acaggaagcc aggtcgcgca
     2881 cggtcatcgg ctcgccgctg ggggcgatat gttctttcaa agggatctcg gtcatcttac
     2941 tctcctcttc ttttctaaaa tgccgtgggc gatcaactga gcggaacggc ctggcctgct
     3001 gtgac
//
LOCUS       DASZAK010000008         3092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_248600, whole genome shotgun sequence.
ACCESSION   DASZAK010000008 DASZAK010000000
VERSION     DASZAK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248600"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VGT44_00115"
     CDS             complement(<1..1520)
                     /locus_tag="VGT44_00115"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV2579225.1"
                     /translation="MTQIIDRKTEHATAGKRTRRQDGYNKLVGRTRYAGDLTFVGLLH
                     ARLVLSPYAHARIVSINTSAALEVPGVKAVYTSQTLGMSDPESSSRTQSPLASKEVLW
                     CGHPVAIVLAESETAAEDGVAAVDVEYEPLDVVVDPISAMRPGAPLTRIFIKETSSEI
                     AGGGAHAAVSEDQVAEADEEELSQNVSDKVHMHAGDMEAGWQEAEVVVERTYTTSIVH
                     QSYMEPQSITVVPDAQTGHRLTIWPSSQGMLGVRSDVAEALTIPERQIRVESVPIGGA
                     FGGKFGLIEPLGAAAAHTSRRPVRLTYTRSEDLFAGNPAPQSIITFKLGAKRDGTLVA
                     MQGSVIFDTGAYAGSAVFLGGLILGSSYRCPNYDFRCYEVMTNKVGVGAYRAPGAPQA
                     TFALESTVDELCHQLQIDPIAFRLKHGLKEGDPSMDHRSTLPPIGLLDCLRKAQEQPL
                     WAERLAQKDTPDELKGWKIGIGVAAGHWPGGTEPAAAACRLESDGTVTVVVGTVDLT"
     gene            1766..3067
                     /locus_tag="VGT44_00120"
     CDS             1766..3067
                     /locus_tag="VGT44_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEV2579226.1"
                     /translation="MPEIDSDHVFGFDTLAIHAGQRPDPTTGARAVPIYQTTSFVFDD
                     TDHAAHLFALQRFGNIYTRIMNPTNAAFEERIAALEGGTGALATASGMAAQMVTMMTL
                     LRPGDEMVSSSSLYGGTHTQFDLTLRTWGINTIFVDSTDPENYRRALTPRTRALYGET
                     IGNPRGDVLDIRAVAGIAHEAGVPLVVDNTFATPYLCRPLEYGADIVLHSATKFIGGH
                     GTSIAGVLVESGKFPWDNGNFPHIVDPSPGYHGVRFYETFGDFAFVMKARVETLRDTG
                     PCISPFNSFLLLQGLETLSLRMKQHCQNALQVARFLQEHPAVSWVHFPGLADDPSYTL
                     AQRYLPRGAGSIFTFGVKGGYEAGKRVINNVRLFSHLANVGDARSLIIHPASTTHQQL
                     SEEDQLAGGVTPDLIRLSIGIEDVEDILWDLDQALGKTSSM"
BASE COUNT          683 a          878 c          834 g          697 t
ORIGIN      
        1 gtcaggtcaa cggttccaac gacgacggtg acggtgccat cggactccag gcggcaggca
       61 gccgcggccg gttcggtgcc gccaggccag tgaccagcgg caacacctat acctattttc
      121 cagcctttca gttcgtcagg tgtgtctttc tgcgccaatc gttcagccca gagcggttgt
      181 tcctgcgctt tccgcaggca atcaagtaag ccgataggcg gcagcgtgct gcgatgatcc
      241 atgcttggat cgccctcttt caagccatgc ttcaggcgaa aagcgatagg atcaatctgc
      301 aattgatggc acaattcgtc aacggtcgac tccagcgcga aggtggcttg tggggcgcca
      361 ggagcacgat atgcaccaac gcctacctta ttggtcatca cctcataaca gcggaagtca
      421 taattggggc agcgatagct actgccgagg atgagtcccc ccaggaagac cgccgaaccg
      481 gcgtacgctc ctgtatcaaa aatgacgctt ccctgcatcg ccacgagcgt accatcacgt
      541 ttcgcgccaa gcttaaaggt gatgattgat tgcggggcag gattaccggc aaagagatct
      601 tctgaacgtg tgtacgtcag gcgcacgggt ctgcgcgagg tatgagcggc agcagcgcct
      661 aaaggctcga taagcccaaa ttttccgccg aaggctcctc ctataggcac tgactctacc
      721 cgtatctgcc gttcgggaat ggtgagcgct tcagctacat ccgagcgcac gccaagcata
      781 ccttgagagc tgggccagat ggtcagccgg tgtcccgtct gggcatccgg cacgacggta
      841 attgactggg gttccatgta ggactggtgt acgatgctcg ttgtataggt gcgttcgaca
      901 actacttctg cctcttgcca acccgcttcc atatcacctg catgcatatg caccttatcg
      961 ctcacatttt gcgaaagttc ttcctcgtca gcttctgcta cttgatcctc tgaaacagcg
     1021 gcgtgagcgc ctcctcccgc gatctccgag gaagtttcct tgatgaagat tcgcgtcagg
     1081 ggcgcgcctg gccgcattgc agagataggg tctactacca catccaacgg ctcgtattcg
     1141 acatccacgg cggctacacc atcttcggcg gctgtttcgc tctcagccag cacgatggcg
     1201 acgggatgac cacaccagag gacctctttt gacgccaggg gggactgcgt gcgcgagcta
     1261 ctttcggggt ccgacatgcc caatgtctgg ctggtatata cagccttcac gccaggaact
     1321 tccaacgctg ctgacgtatt gatgctgacg atacgcgcgt gagcataagg gctaagtacc
     1381 aacctggcat gcagtaaacc tacgaaagtc aggtcaccag catagcgggt acggcccacc
     1441 aatttattgt aaccatcctg ccggcgcgtc cgcttgccag cagtggcgtg ttctgtcttc
     1501 ctgtcaatga tttgtgtcat gcaatactcc cattctcgtt cgagatacgc taatcgttaa
     1561 ttacacccga cacgggcgac cataaaggcc caccacagcg ctcctcactg ccctcacccc
     1621 tacaacacag gtggaccatt cttagaaccc ttgtgaaagt gtagcacaaa atcggcggtc
     1681 gcgacaggag attctttctt tcctgtcaaa cgtccatatg ataggatatg gaagaacagg
     1741 cacctgggaa aggagcaaat acaccatgcc agaaatcgat agcgatcatg tttttgggtt
     1801 tgataccctg gcaatacacg cgggacagag accagaccct accacgggcg cgagagctgt
     1861 tccaatctat caaacgacct cgtttgtctt tgatgacacc gaccatgctg cacatctctt
     1921 tgccttacaa cgctttggca atatttatac gcgcatcatg aatcccacca atgctgcttt
     1981 tgaggagcgc attgcggctc ttgaaggcgg gacaggcgcg ctggcaacgg cttcgggcat
     2041 ggccgcgcaa atggtgacca tgatgacctt gctgcgtcca ggggacgaaa tggtctcttc
     2101 gagcagtctt tatggtggca cgcacacaca gtttgacctg acactgcgta cctggggaat
     2161 caatacgatc tttgtagata gcaccgaccc ggagaactat cgacgcgctc tgactcctcg
     2221 cacgcgtgcc ttatatggtg agacgatagg caatccacgg ggcgatgtgc tggacatccg
     2281 cgcggttgcc ggtattgcgc atgaagcagg tgtgcccctg gttgtcgata atacctttgc
     2341 gactccctac ctgtgccgcc cactcgagta cggagccgat atcgttttgc attcagcgac
     2401 caagttcatc ggcggccatg gcacttctat cgcgggagtg ctggtcgaat cggggaagtt
     2461 tccctgggat aacggcaatt tcccgcatat cgttgacccc tccccgggct atcatggcgt
     2521 gcgtttttat gagacgttcg gcgactttgc ctttgtgatg aaagccaggg tcgagacgtt
     2581 acgcgataca ggcccatgca tcagcccttt caattcattc ctgctgttac aggggctgga
     2641 gacgctatcg ctgcgcatga aacagcactg tcagaatgcg ctccaggtgg cacgcttcct
     2701 ccaggagcat ccggcagtca gttgggtaca ttttccaggt ttagcagacg atccttcata
     2761 cacgcttgcc cagcgctatt tgccacgagg cgctggatcg atcttcacat ttggcgtcaa
     2821 aggtggatat gaagccggga agcgggtgat caacaatgtg cgcctgtttt cgcatctcgc
     2881 caacgtgggt gatgcgcgca gcctcattat tcatcccgcc agcacaacgc accagcaatt
     2941 gtcagaagaa gaccagttag caggtggagt gacgcccgac ttgatccggc tctccatagg
     3001 aattgaggat gtggaagaca tcctatggga cttagaccag gcgttgggga aaacaagtag
     3061 catgtgatcc agggcggcgc aagcgccaat ac
//
LOCUS       DASZAK010000009         1768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_390593, whole genome shotgun sequence.
ACCESSION   DASZAK010000009 DASZAK010000000
VERSION     DASZAK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390593"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="VGT44_00125"
     CDS             complement(<1..666)
                     /locus_tag="VGT44_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579227.1"
                     /translation="MKLVRNRFAKHAVVLGVSLTLLVSLLITLPLAVRAAQHQNIRAL
                     APADMQNLIFVWASGTPAVQQQALIICQAEAITPANCAAISADVRSAWLALMQVDPAS
                     LGRIGVQDNPVGRTQVYSVLAGQLSSLTQGNEILLLSQTQQTLQLLDASLQQVNGALG
                     SAGATSYTVWATSFKQKSKTGRYVALPDAYLKFANEGAISSIPSIYQTYYAPNGVKTS
                     WSVS"
     gene            complement(1015..>1768)
                     /locus_tag="VGT44_00130"
     CDS             complement(1015..>1768)
                     /locus_tag="VGT44_00130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2579228.1"
                     /translation="AQPLSDRMMELAHIAPGQRVLDVATGIGEPAMTAARRVGPGGSV
                     VAIDQAPQMLAVARERMQAAGIRTVEFVEGDAEAVTLPIDTFDAVVCRWGLMFFHDPV
                     GVLARFRNSLVPGGWLAAAIWGEPSRVPIISLPFSVLSRELGQPPSLPSGPSPFALSN
                     PTRLEQVLRDAGFAQVRSEPFTVTFVFASVDELLGHLEEVSAPIRMTMATSSPEYQAE
                     FWKKLAEAAVPFIDADGIIRLSNDCLVVAGQR"
BASE COUNT          370 a          511 c          508 g          379 t
ORIGIN      
        1 cgagactgac caactcgttt tgacgccatt gggcgcataa tatgtctgat agatggacgg
       61 tatactcgaa atggctcctt cgttcgcgaa cttgagatag gcatcgggca gcgccacgta
      121 acgaccggtc tttgatttct gcttaaagga ggttgcccaa accgtatagc tggtcgcgcc
      181 tgctgatccc agagcgccat tcacctgttg taaagaagcg tccaatagtt gcaaggtctg
      241 ctgtgtctgt gacagcagca atatttcgtt gccctgggtc agcgaggaaa gctgtcccgc
      301 aaggacgctg tatacctgtg ttctgcctac aggattgtct tgcacgccaa ttcttccaag
      361 ggacgccgga tcgacctgca ttaaggcgag ccaggcgctg cggacatctg cgctgatcgc
      421 ggcgcaatta gcgggcgtaa tggcctccgc ctggcaaatt attaatgctt gttgctggac
      481 cgccggtgtg ccactggccc acacaaagat aagattttgc atatccgccg gtgcaagcgc
      541 gcgaatattc tgatgttgag ccgcgcgcac ggccaatgga agcgtaatca acaaacttac
      601 taataaagtg agggacactc ctagaactac tgcatgtttc gcgaaacgat ttctcactaa
      661 tttcatatgc ctacagattt cctttgctac gactgctttc ttacaacgat ggatgttcga
      721 ttatgcgcag aagaatctgg cacatagaga tgggttttgc agactcatgc taccatagta
      781 gaacgaatga aatcagactg cctgcttgta acgtgacttt attccttgtg gtggcttgcc
      841 gatatcaata ccggcctttt tcaattcgcg accgagttct ctgcggaagg cgtggcagtc
      901 aacctctata gagtggcggg tgccgccggt aaaggcgaag tgggatggtc gatctcatgc
      961 tgaatggcgg cagaggagat gagcaggttc tgcgcgcctc ctcctgttgc aggctcagcg
     1021 ctggcccgcg acgaccagac aatcgttcga caggcgaatt atcccgtcag catcgatgaa
     1081 tgggactgcg gcttcggcga gcttcttcca gaactcggcc tggtactcag ggcttgatgt
     1141 ggccatggtc atgcgaatcg gcgccgagac ctcttccagg tgtcccagca gttcgtccac
     1201 cgaggcaaac acgaaggtta cggtaaacgg ctcgctccgc acctgcgcga agccagcgtc
     1261 tcgcagtacc tgctccagtc tcgtcgggtt ggacagtgcg aaggggcttg gtccactggg
     1321 gagcgagggc ggttggccca gctcgcggct gagcactgag aatggtaatg agatgatcgg
     1381 cacccgcgaa ggttcacccc aaatggcggc ggcgagccac ccacctggaa cgaggctgtt
     1441 cctgaagcgc gcgagtacgc ccacaggatc gtggaagaac atcagccccc agcggcagac
     1501 taccgcatcg aaggtgtcga tcggcagcgt cactgcctca gcgtcccctt ccacgaactc
     1561 gacggtccgt atcccggcag cctgcattcg ctcgcgcgcc acggccagca tctgcggcgc
     1621 ctggtcaatt gccaccactg agccgcctgg ccccacccgg cgcgccgccg tcatggccgg
     1681 ttcgccgata cccgtcgcca cgtcgaggac ccgctggccg ggcgcgatgt gagccagctc
     1741 catcatccga tcgctgagcg gctgcgcc
//
LOCUS       DASZAK010000010         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_142149, whole genome shotgun sequence.
ACCESSION   DASZAK010000010 DASZAK010000000
VERSION     DASZAK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142149"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VGT44_00135"
     CDS             complement(<1..580)
                     /locus_tag="VGT44_00135"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579229.1"
                     /translation="MPGQTSRRLLFYTLYLAYVAGGIIVILPGPTLSLLAANTGVSLE
                     QGSWIFTSSSTGFALGAFVAGMLDRKVSPKTLLMIGLVVMGSMGIITPYTHSFPILLF
                     SQLTKGIGFGIMDVSINILSTLAFHDTLSETLNGLHSSFGIGSLVAPLVLSVTLTLLN
                     DFTWAYVIGSVGALLCAGMLLPQRVPISVQTSA"
     gene            complement(584..1576)
                     /locus_tag="VGT44_00140"
     CDS             complement(584..1576)
                     /locus_tag="VGT44_00140"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV2579230.1"
                     /translation="MLELHGNALEEHGSELLATLVELAHRQPEAAVVRAVLLAAPGTP
                     LPDLASVGALGIEHVYVLAHPRLARYATEAWVSALAWLVRERAPLLVASGATANGRDW
                     MPRLAARLQLPYAPGCLSIDVQGDALYALRPIYEGRAYVQTRASLKGRSALATLLMDT
                     RGNPVAMEESSQGHQSPLVTTFTPVIEASNMAEQVQQVAIQAPSPEEVELEEAERIVA
                     GGRGVGQEGFARVAAFARMLGAAVGATRVSTDRGWVEHARQIGATGKSVHPRLYIACG
                     ISGAAQHTSGMREAQTIIAINPDRTAPIFALADLGLLGDANQVVPLAAKLIAKD"
BASE COUNT          341 a          554 c          535 g          303 t
ORIGIN      
        1 cagcggaggt ctgtacgctg ataggcacgc gctggggtag caacatgccg gcgcagagca
       61 gcgcgccaac ggagccgatc acatacgccc aggtgaaatc gttcaacaga gtcagcgtga
      121 ccgatagcac caggggcgca accagcgagc caatgccgaa cgagctgtgc agcccgttca
      181 atgtttcgct caaggtgtca tgaaatgcca gcgttgacag gatattgata ctgacatcca
      241 taatgccaaa gccgatgccc ttcgtcagtt gcgagaagag caggatggga aacgagtggg
      301 tgtaaggagt gatgatgccc atgctgccca tgaccaccaa tccgatcatc aataaagttt
      361 tggggctgac cttcctgtct agcattcccg cgacgaatgc tcccagcgcg aagccggtgg
      421 acgaggaggt aaatatccag ctgccctgct ctagcgagac gcccgtattg gccgccagca
      481 gcgaaagcgt cggccccggc aaaatgacga tgatgccccc cgcgacgtat gccaggtaaa
      541 gcgtgtagaa aagcaatcga cgactggttt gaccaggcat gagttaatcc tttgcaatca
      601 acttcgcggc gagtggcacc acctggtttg catcgccgag cagaccgaga tcagccagcg
      661 caaagatggg cgcggtgcga tcagggttga tggcgatgat ggtctgagcc tcgcgcatcc
      721 cgctggtgtg ttgagccgcg ccggagatgc cgcaggcgat gtacagccgg ggatgcacac
      781 ttttgcccgt cgcgccaatc tgcctggcgt gttccaccca gccgcgatcc gttgacacgc
      841 gcgtcgcccc gacagccgcg ccgagcatgc gcgcgaaggc ggcgacacgc gcaaatcctt
      901 cctgcccgac gccgcgcccc ccggccacga tgcgctccgc ctcctccaac tctacctctt
      961 ccggcgatgg cgcctggatt gcaacctgct gcacttgctc ggccatattc gacgcttcga
     1021 tgaccggcgt gaacgttgtc acaagcggcg attgatgacc ctggctgctc tcttccattg
     1081 cgaccgggtt gccccgcgta tccatcagca gggttgccag cgcgctacgc cccttcagtg
     1141 aggcgcgtgt ctgcacatag gcgcggcctt catagatcgg gcgcagggcg tagagggcat
     1201 caccctgcac atcgatgctg aggcagcccg gcgcgtaggg gagttgcagg cgcgccgcca
     1261 ggcggggcat ccagtcccgc ccgttggccg tcgccccgct ggcgaccagc agcggcgctc
     1321 gctcccgcac cagccacgcc agggcgctga cccacgcctc cgtcgcatag cgcgccagtc
     1381 ggggatgtgc gaggacgtag acatgctcga tgcccaacgc gccgactgac gcgaggtcgg
     1441 gaagcggcgt acccggcgcg gcaagcagca cggcgcgaac aacagccgct tccggctgcc
     1501 gatgcgccag ttcaaccagc gtcgcgagta attcgctgcc gtgttcttcg agtgcgttgc
     1561 cgtgtagttc gaggattccc cagagttctt acatgtgttg cctttgtagt ggtgtcatca
     1621 gccaacccgt aggagccggt ttaccgtgct caccgccgat tatgcaagtg aacaacatga
     1681 cccggccacc ggagcgggtt ggacgagggc gaccgcaagg gtctccaccc cac
//
LOCUS       DASZAK010000011         4616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_319634, whole genome shotgun sequence.
ACCESSION   DASZAK010000011 DASZAK010000000
VERSION     DASZAK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4616
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319634"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..901)
                     /locus_tag="VGT44_00145"
     CDS             complement(<1..901)
                     /locus_tag="VGT44_00145"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEV2579231.1"
                     /translation="MADSDIEQLRDAIDRAWEEARNFGLDPFPTHFELVPASIMYEFA
                     SYSLPGRFSHWTHGKAYHRQKMQYDFGLSKIYEMVVNTNPSYAFLMEMNNLLQQTFVV
                     AHVFGHTDFFKNNAYFQHTSRRMIDKVSIHAERIAKYEFDHGKAEVERFLDAVLSIQE
                     HVDYNLFIRENEPEKAEKDKSGEQPRAKPSSEYDDLWGLEKKAKKAEEERDRQPGKPP
                     KFPEKPEKDLLRFLMRYAPHLQPWQRDIIDIVRTEMLYFVPQMQTKPLNEGWASTWHS
                     RIMRRLGETGAISDSDTIEFAQLH"
     gene            complement(948..2090)
                     /gene="yhbH"
                     /locus_tag="VGT44_00150"
     CDS             complement(948..2090)
                     /gene="yhbH"
                     /locus_tag="VGT44_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YhbH"
                     /protein_id="HEV2579232.1"
                     /translation="MSVSQHDWSLHRKGQVDQERHKEKIREAIKKNLSDIVSEESIIL
                     SDGRKMVRVPVRSLDEYRFRFDPGRQTHAGEGNGKSKVGDVVAQEPRPGKGKKGDAGK
                     DAGYDYYEAEITMEELAALIFEDLGLPNLEQKRQQELQTEAVRFTDVRKKGPLTNLDK
                     KRTIMENLKRNAAKGDAKFKDIKSEDLRFKVWEPTIKYQSNAVVIAMMDVSGSMGEFE
                     KYIARSFYFWMVRFLRTKYNNVQIVFISHHTEAKEVTEEEFFHKGESGGTQVSSAYEL
                     ALEIIKERYNPDDWNIYPFHFSDGDNLPWDNDRCVQMVNRLMELCNIFGYGEIREGHY
                     RSPSTLMSAYNKINDKKFIAVTISDKKEVYPALRKFFAQRDPIPTR"
     gene            complement(2147..>4616)
                     /locus_tag="VGT44_00155"
     CDS             complement(2147..>4616)
                     /locus_tag="VGT44_00155"
                     /inference="COORDINATES: protein motif:HMM:NF026240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LAGLIDADG family homing endonuclease"
                     /protein_id="HEV2579233.1"
                     /translation="YLQLPNKPRDDEFVPYETTVLGIDGPEQASKFYCGGFQPIYEVE
                     TNLGYTIRGTANHPLLTLQPGGELAWTKIGDLHPDDYIALARGSRIFGQSAALPESFY
                     PLPRQPRTMTPELAYWLGLLTAEGSVTPYETWFVNGNQALIDRFVELTRDLFGLEAVP
                     HRKAETYNYNISISSKALSAWLRGELGIARGSHAKSIPACVLASSEQDILAFLEGLFW
                     GDGTIRGNKAGYNTFKYTSKSRQLARQAHIALLNLGVVGSFWSHTDEGETYYNVTLRG
                     DQVLDLVELIPSLREKATSPLRESRSNKTIYDHLPHGTTLLNGHGGDSYLARILTGDR
                     QLSYNRARTVLAAQPELAHSALATLVKQNFLWLTVREIRAAGIEPVYDLLVPGTHSFV
                     ADGFVQHNSQDISELVGGIDLSTIGDVGVESDPRAYRFDGELNIANRGIMEFVEMLKT
                     DEKFLYVLLTLSQEQNIKTGRFSMIYADEVVVSHTNEHEYQSFVGNKKSEALQDRIIL
                     VKVPYNLRVSDEIKIYEKLLKQSALQNVHIAPYTLRVASMFAVLTRLEQSKKAGMSAM
                     KKLKLYDGEDMEDFKQKDIKELQDEAVREGMDGISPRYVINRLSSALVKQNTTCINPI
                     DALRALRDGLDQHTSITREEREHYLNFISEARKEYDDMARKEVQRAFVYSYEESARTL
                     LNNYLDNVEAFCNKTRLRDPITDEEMEPDEQLMRSIEEQIGVTENSKKSFREEILIRI
                     SMLARKGMTFDYTSHERLKEAIEKKLFADLRDVVKITTSTRTPDKEQLRKINEVVDRL
                     MTEHGYCHVCANEILRYTGSLLNR"
BASE COUNT          826 a         1232 c         1400 g         1158 t
ORIGIN      
        1 agtgcagttg ggcaaattcg atggtgtcgg aatcggagat agcgcccgtt tcgcccaggc
       61 ggcgcatgat gcgcgagtgc caggtactgg cccacccctc gttcagtggc ttggtttgca
      121 tctgcggcac gaagtagagc atttcggtgc gcacgatgtc gatgatatcg cgctgccagg
      181 gctgcaagtg cggggcatag cgcatcagga atcgcagcag atctttttcc ggcttctccg
      241 ggaatttagg cggcttgccg ggctgccggt cgcgttcctc ttccgctttt ttcgcctttt
      301 tctccaatcc ccacaggtca tcgtattcgc tgctcggttt ggcacggggt tgctcgccgg
      361 atttgtcttt ctcagctttc tccggctcgt tttcgcggat gaagaggttg tagtctacat
      421 gttcctggat cgaaagcacg gcatccagga agcgttctac ttcggctttg ccatgatcga
      481 attcgtattt ggcgatgcgc tcggcgtgga tgctgacttt gtcgatcatg cggcgcgagg
      541 tatgctggaa gtaggcgtta tttttgaaaa aatcagtatg gccaaagaca tgcgccacga
      601 caaaggtctg ctggagcagg ttattcattt ccatcagaaa ggcatagctg ggattggtat
      661 tgaccaccat ctcgtagatt ttgctcagtc caaagtcata ttgcatcttc tggcggtgat
      721 aggctttgcc atgcgtccag tggctgaaac gtcccggcag gctgtacgag gcaaattcat
      781 acatgatcga cgccggaacc agctcgaaat gcgtagggaa cgggtcaagg ccgaaattgc
      841 gggcttcttc ccaggcgcga tcaatggcgt cgcgtaattg ttcaatatcg ctgtctgcca
      901 tgtggaaaat ctcctatggg gacgcgatgc atcgcgtccc cacgaattta tctagtgggt
      961 atgggatcgc gctgcgcgaa gaacttcctc agcgcagggt agacctcctt tttgtcagag
     1021 atcgtaaccg cgatgaattt cttatcgttg attttattgt aggcggacat cagggtgctg
     1081 ggcgagcgat aatgtccctc gcgaatttcg ccgtagccga agatgttgca gagttccatc
     1141 agcctgttga ccatttgtac gcagcgatcg ttgtcccagg gcaggttgtc gccatcggag
     1201 aagtggaagg ggtagatgtt ccaatcgtcc gggttgtagc gttccttgat aatctccaat
     1261 gcaagctcgt aggcggacga gacctgcgtg ccaccgctct cgcccttgtg gaagaattcc
     1321 tcctccgtga cttccttcgc ctcggtatgg tggctgatga agacgatttg tacattgttg
     1381 tacttggtgc gcaggaaacg caccatccag aagtagaagc tgcgcgcgat atatttctcg
     1441 aattcgccca tcgagccgct gacgtccatc atggcgatta cgaccgcgtt tgactggtat
     1501 ttgatcgtgg gttcccagac cttgaagcgc agatcttcgg atttgatatc tttgaacttc
     1561 gcatcgccct ttgcagcgtt gcgtttcagg ttctccatga tggtgcgttt tttgtccagg
     1621 ttggtgagcg ggcctttctt gcgcacatcg gtgaagcgca cggcttcggt ttgcagttct
     1681 tgctggcgtt tttgctcgag gttgggcagg ccgaggtctt cgaagatcaa cgcggccagc
     1741 tcttccatgg tgatctcggc ctcgtagtaa tcgtagcctg cgtccttgcc cgcgtcgcct
     1801 ttcttgccct tgccggggcg cggctcctgc gccaccacgt cgcccacctt gcttttgccg
     1861 ttgccctcgc ccgcatgggt ctggcgaccg gggtcgaaac ggaaacgata ctcgtcaagg
     1921 gagcgcacgg gaacgcgcac catcttcctg ccatcggaga gaatgatgga ttcctcgctg
     1981 acgatatcgc tgaggttttt cttgatggcc tcgcggatct tctctttatg acgttcctgg
     2041 tccacctgtc ccttgcggtg cagcgaccag tcgtgttgtg aaacggacat agaacacgct
     2101 ccttgtggca ggcgaccggc ggtaaagcgc cggttgtcgg gttcttctag cggttcaaca
     2161 gactgccggt gtagcgcagg atttcgttag cgcagacgtg gcagtagcca tgctcggtca
     2221 tgaggcgatc aaccacctcg ttgatcttgc gtagttgctc tttgtcgggc gtgcgtgtag
     2281 atgttgtgat cttcaccacg tcgcgcaggt cggcaaacag cttcttttcg atggcttcct
     2341 tcaaccgctc gtggctggtg tagtcaaagg tcataccttt gcgcgccagc atcgagatgc
     2401 gaatgaggat ctcttcgcgg aaggacttct tggagttctc ggtcacgccg atctgttctt
     2461 cgatggagcg catcaactgc tcgtccggct ccatctcctc gtcggtgatg gggtcgcgca
     2521 gccgggtctt attgcagaac gcttcgacgt tgtcgaggta gttgttgagc agcgtgcgcg
     2581 ccgactcctc gtaggagtag acgaaggcgc gctgtacctc tttcctggcc atgtcgtcgt
     2641 attctttgcg cgcctcgctg atgaaattca ggtagtgctc gcgttcctcg cgcgtgatgc
     2701 tggtgtgctg gtcgaggcca tcgcgcagcg cgcgcagagc atcgatgggg ttgatgcagg
     2761 tggtgttttg cttcaccagc gcgctggaga ggcggttgat gacgtagcgc ggcgagatgc
     2821 cgtccatgcc ctcgcgtacc gcttcgtctt gcaactcctt gatgtccttc tgcttgaagt
     2881 cctccatgtc ctcgccgtcg tagagcttga gcttcttcat ggcgctcatg cccgccttct
     2941 tggattgctc caggcgggtc aacacggcga acatgctggc gacacgcagg gtatatggtg
     3001 cgatatgcac gttctgcaag gcgctctgtt tgagcagctt ctcgtaaatc ttgatttcat
     3061 cggagacgcg caggttgtac ggcactttga ccagaatgat acggtcttgc agcgcctccg
     3121 actttttatt accgacgaaa gactggtatt cgtgttcgtt ggtatggctg acgaccacct
     3181 cgtcggcgta gatcatgctg aaacgtcccg tcttgatgtt ctgctcctgg ctgagcgtga
     3241 gcaggacgta gaggaatttc tcgtccgtct tcagcatttc tacgaattcc ataatgcccc
     3301 ggttggcgat gttcagctcg ccatcgaatc tgtacgcgcg gggatcgctc tcaacgccta
     3361 cgtcgccgat ggtggagagg tcgatgccgc cgacgagttc gctgatgtcc tggctgttat
     3421 gctgcacgaa gccgtcggcg acgaacgagt gcgttcccgg cacgagcaaa tcatagaccg
     3481 gctcgatgcc agcggcgcgt atctcgcgga ccgtcagcca gaggaagttt tgcttgacca
     3541 gcgtcgcaag cgcgctgtgc gccagttctg gctgagcggc aagaacggtg cgcgcgcggt
     3601 tgtaagaaag ttgccgatcg ccggtgagga tgcgcgcgag gtagctatcg ccgccatgtc
     3661 cgttgagcag ggtcgtgccg tgcgggagat ggtcgtagat ggtcttgttg ctgcgcgact
     3721 cgcgcagggg tgatgtggct ttctcccgaa gcgatgggat gagttctacg agatcaagca
     3781 cctggtcgcc gcgcagggtg acattgtagt aggtctcgcc ctcgtcggta tggctccaga
     3841 acgagccgac cacgccgaga ttgagcagcg cgatatgcgc ctggcgggcg agttggcgcg
     3901 acttgctggt gtacttaaat gtgttataac cagctttgtt gccgcgaatg gtgccatcgc
     3961 cccagaagag accttccagg aatgccaata tatcctgttc gctgctcgcc aggacacagg
     4021 ccggaataga cttggcatgg gagccgcgcg cgatgcccag ctctccgcgt agccacgcgc
     4081 tgagggcttt gctactgata gagatgttgt agttgtaggt ttccgccttg cgatgtggca
     4141 cggcttccag accaaacagg tcgcgagtca actcgacaaa gcgatcgata agcgcctggt
     4201 tgccattgac gaaccaggtt tcatagggcg tgacgctgcc ttccgctgtg aggagaccca
     4261 gccagtaagc cagttcgggc gtcatggtgc gcggctggcg tggcagtggg tagaagctct
     4321 ccggcagcgc tgcgctctgg ccaaagatgc gactgccgcg cgccaacgct atgtagtcgt
     4381 caggatgcag gtcgccaatc ttcgtccagg ccaactctcc gccgggctgc aaggtgagca
     4441 gtggatggtt tgcggtaccg cgtatagtat agccgaggtt cgtctcgacc tcgtagatgg
     4501 gctggaagcc gccgcagtag aacttgctgg cttgctccgg gccgtcgatg cccaacacgg
     4561 ttgtttcgta aggcacgaac tcgtcatcgc gaggtttgtt aggaagctgg aggtag
//
LOCUS       DASZAK010000012         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_390662, whole genome shotgun sequence.
ACCESSION   DASZAK010000012 DASZAK010000000
VERSION     DASZAK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390662"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..631)
                     /locus_tag="VGT44_00160"
     CDS             complement(29..631)
                     /locus_tag="VGT44_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2579234.1"
                     /translation="MTTDSSTGDSARVHKDYYTALCSAALSFSSSLELKEVLQNVVTN
                     VTETMKVKACSLRLYDSRTGQLQLSAAHGLSSSYLAKGPVDIQHSAIDSDALAGETVI
                     IQDVRTDTRFQYAQAAIREGIVSILCVPLEVHGEAIGVMRVYTGEPCTFHQDDIQFLS
                     VLARLAALSIENANLYESVRNSYDGVMEVLWGSSLQPADN"
     gene            complement(819..1310)
                     /locus_tag="VGT44_00165"
     CDS             complement(819..1310)
                     /locus_tag="VGT44_00165"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2579235.1"
                     /translation="MVHFWRRAKDEIETSKTAPGVGASGDIAVIVDGKKLDNELVRLA
                     CMMAKRAKRKVHLVHIIEVPRTLPLKASLTQESENADKLLNEAIAIADEAGCEAIAEV
                     VQTRDAGPAIVEEARDHACALIMLGLVRHTQKGTTNTLGKTVPYVLEHSPCRVWVVQD
                     SVL"
     gene            complement(1494..1796)
                     /locus_tag="VGT44_00170"
     CDS             complement(1494..1796)
                     /locus_tag="VGT44_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEV2579236.1"
                     /translation="MSSETTTLSQVELEELTRLREKLLPPYNLILFNDDYNDMLYVVA
                     VLLHTINRLSRREAEHIMLTAHLTGSAVVVTCPKETAEHYQERLLGYGLTATIEPD"
     gene            complement(1931..2500)
                     /locus_tag="VGT44_00175"
     CDS             complement(1931..2500)
                     /locus_tag="VGT44_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579237.1"
                     /translation="MQQQELNYDLLAVFGSETDADAAAVKLTKEGFGDDEVFRMTGDS
                     VTSGEFRDHGPNRNRSEVFLQTSRSGPNPVSVIALALLFGVLIGGIMFGAAEFTTKLL
                     PIVPSTVAGAAVGIILGAVLGLLQRGRERGNIGQDRSRQAAAQQNQGARTVIAVRLSN
                     AENISRKSKARAILINNNGRIDRSVGREV"
BASE COUNT          623 a          757 c          750 g          668 t
ORIGIN      
        1 gacgcgatga tgaaattgtc gatttcccct aattgtcggc gggttgcaag ctgctgcccc
       61 acagcacctc catgacgccg tcgtaagaat ttctcacact ttcgtacaga ttggcgtttt
      121 caatggagag cgccgcgagt cgcgccagta cgctgaggaa ctggatatcg tcttgatgga
      181 aggtgcaagg ttcgccggta tagacgcgca tcacgccgat tgcttctcca tgtacttcaa
      241 gcggcacaca gaggatggag acgatgcctt cgcgaatggc tgcctgggca tactggaagc
      301 gggtatcggt gcgcacgtcc tggataatga ccgtttcgcc tgcgagcgca tcagaatcga
      361 tcgcgctatg ctggatatcg accgggcctt tcgccagata actgctgctc aatccatgag
      421 ccgcgctgag ttgcagttgc ccggtacgcg aatcgtacag gcgcagcgaa caggctttga
      481 ccttcatcgt ctcggtcacg ttcgtcacga cattttgcaa cacttctttc agctccaggc
      541 tggatgaaaa cgagagcgcg gcagaacaca gcgcggtgta ataatcctta tgcacgcggg
      601 ctgaatcgcc tgtagaagaa tcagttgtca tgtgtaatcc cctcctgtag gtaggtgcag
      661 tgaggaagtt gggagctacc ctctctaacc cctgcaccct gaccgcgcaa gccgcttgta
      721 aaaatgcatc aaaaaagagc agtacgatat gtacaattat accatacctt gccgtttgcc
      781 agcacgatcg aggtggatgc cggcaacatg tgtcagtgtt acaacaccga gtcttgtact
      841 acccagacac ggcagggaga atgctcaagc acatacggaa cggttttgcc aagcgtgttg
      901 gtcgtgccct tctgggtatg gcgaaccaat cccagcatga taagcgcaca ggcatgatcg
      961 cgcgcctctt cgacaatggc cgggccagca tcgcgggtct ggacgacttc ggcgatggct
     1021 tcgcacccgg cttcgtcggc aatggcaatc gcctcgttca gcagtttgtc ggcgttttct
     1081 gattcctggg tgagagaggc tttcaaagga agggtacgag ggacttcaat aatgtggacg
     1141 aggtggactt tgcgtttggc gcgcttcgcc atcatacagg ccaggcgcac cagttcgttg
     1201 tccagcttct tcccgtctac gatgacggcg atatctcccg atgctccaac ccctggagca
     1261 gttttgctcg tttcgatctc gtctttggct cttcgccaga aatgaaccat aatgactctc
     1321 ctcctgcagg aacatgataa aacagcatca tttcttgcgt cgtatctcta caatgaacta
     1381 tcgtcgatac ggttccttca ggttactatt gattctaacc cctaaccagt tgatcgtcaa
     1441 tagttgatgc gccccatttc tccgcagagt cgcacggaaa ctcacatatt gatttagtcg
     1501 ggttcaatgg tggccgtcaa gccataaccg agcaagcgct cctggtagtg ttccgccgtc
     1561 tctttaggac aggtcacgac tacggcgctg ccggtcaaat gggcggtcag catgatatgc
     1621 tcggcctcgc ggcgcgaaag ccggttgatg gtatgcagga ggactgcgac cacgtacagc
     1681 atgtcattat aatcgtcgtt aaacaggatc agattgtaag ggggaagcag cttttcgcgc
     1741 aggcgagtaa gctcttctag ctcaacctgg ctgagcgtcg tagtttcact ggacatcgag
     1801 ggcatctccc gtaacaggct ataaaaccgg aatgaactgg acggtaatta ctatctctat
     1861 tgtaaacgag cgaaccaacc atgacgcgct tttttggaga agggtactac gaggatgaca
     1921 tccgccagag ttacacttct cgtccaacgc tgcggtcaat tctcccgttg ttgttgatca
     1981 aaatcgcgcg cgctttcgat ttgcggctga tgttttcagc gtttgacagg cgcactgcga
     2041 tgaccgtgcg cgcgccctgg ttctgttggg cggcggcctg acgcgagcga tcctgtccga
     2101 tattgccgcg ctcgcggcca cgttgcagta atcccagcac cgcgcccaga atgatgccga
     2161 ccgcggctcc ggcgactgtt gatgggacaa taggcagtaa ctttgtggtg aattcggctg
     2221 ccccgaacat gatgccgccg atcagcacac caaagaggag cgccagcgcg atcaccgaca
     2281 ccgggtttgg cccggagcgc gatgtttgca ggaagacctc gctgcgattg cgattcgggc
     2341 cgtgatcgcg aaattcgccg gatgtcacgc tgtctccggt catgcgaaag acttcatcat
     2401 ccccaaagcc ctctttagtg agtttcactg cggctgcatc ggcgtcggtt tccgacccga
     2461 acacggccag caggtcatag ttcaattcct gttgttgcat cacgatctcc tacatactga
     2521 cgaaataatt gctgcttcct acgacattat aacgaaatat tggcggtatg ggtagtatcc
     2581 cgtcttatga acatcaggca tctctgtagg tgccgattga tcgcgcacat cgccgattta
     2641 tcggcctgat ttagtgccat ccatgccgct cgtggccgat aaatcggcga tgtgcgcgat
     2701 aaatcggcac ctacagagat gcctgatgtt cataagacgg gatactaccc atactgccaa
     2761 tatttcgtta taatagcatg gcgtgatacg caaacgta
//
LOCUS       DASZAK010000013         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_568090, whole genome shotgun sequence.
ACCESSION   DASZAK010000013 DASZAK010000000
VERSION     DASZAK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568090"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /locus_tag="VGT44_00180"
     CDS             complement(<1..1001)
                     /locus_tag="VGT44_00180"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEV2579238.1"
                     /translation="MDVAEIKQLIESGKVEYVKIGAADIEGVFRGKRVAARHFLNSLE
                     EGFAQCDVIFGWDIAENVLPNLKFSNWEHGFSDIVMKPDLSTFAMVPWEENIASCVCD
                     LWTEHGDPVTISPRYVLQQVVKRAREMGFEAFSAAELEFRFFRESQMSLREKDFGPDL
                     TPLNPGFNCYSISQSSADDQLLGRIARMMRDATVEIEGYNREHGPGMYEMNLRYADAV
                     ASGDKTMLFKTGVKEICYQMGYAATFMAKWNDQEDGSSGHSHISLWDINRERNLFWDE
                     GAEGHMSPLMRHFLAGVLEKLPEFMALYAPVINSYKRYVEGTWAPLNTTWGMDNRTCS
                     "
     gene            complement(1078..>1504)
                     /locus_tag="VGT44_00185"
     CDS             complement(1078..>1504)
                     /locus_tag="VGT44_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV2579239.1"
                     /translation="RIEPGDELVTAEIDGITFGLTICYDLRFPELYRLLTLRGADILF
                     VPAAFTLHTGRDHWEVLLRARAIENLCYVVAPAHVGTYPPNRQCFGRSMIVDPWGLIL
                     AQAQDMPGVVIADINLEQIARARAQIPCLENRRPAVYRW"
BASE COUNT          330 a          441 c          431 g          302 t
ORIGIN      
        1 gagcaggtgc ggttatccat gccccaggtc gtattcaagg gcgcccaggt gccttctacg
       61 tagcgtttat aggaattaat caccggggca tacagcgcca tgaactccgg cagcttctca
      121 agcacgcccg ccaggaagtg acgcatcaac ggcgacatat gaccttccgc gccctcgtcc
      181 cagaaaagat tgcgctcgcg gttgatgtcc cacaggctga tatgcgaatg gccgctgctg
      241 ccgtcttcct ggtcattcca cttcgccatg aaggtcgctg cgtagcccat ctggtagcag
      301 atttccttca cgcctgtctt gaagagcatg gtcttgtcgc cggaggctac cgcgtcagca
      361 tagcgcaggt tcatctcgta cataccaggg ccatgctcgc gattatagcc ttcgatctcg
      421 acagtggcat cgcgcatcat gcgggcgatg cgacccaaca actgatcgtc ggcgctcgac
      481 tggctgatgg aatagcagtt gaagccgggg ttgagcgggg tcaggtccgg gccaaaatct
      541 ttctcgcgca gcgacatctg gctctcgcgg aagaaacgaa attcgagttc ggcggcggag
      601 aatgcctcaa atcccatctc gcgcgcgcgc ttcacgacct gctggaggac gtagcgcggc
      661 gaaatagtga cgggatcgcc atgctcggtc cacagatcgc acacgcacga ggcaatgttt
      721 tcttcccagg gaaccatggc gaaagtggag aggtcgggct tcatcacgat atccgagaag
      781 ccatgctccc agttgctaaa cttgaggttg ggcagcacat tctcggcaat atcccagcca
      841 aaaataacgt cgcactgcgc gaagccttct tccagcgaat tcaagaaatg cctggcggcg
      901 acgcgcttgc cccggaagac gccttcgata tcggcagcgc cgatcttgac gtattcaact
      961 ttacccgact cgatcagctg tttgatttca gcaacatcca tgtaagctat tcatcctttc
     1021 tgtgggtgac gacaaaaaca aatacgtaag cccgctacgt agaacatgca aatggcttca
     1081 ccaccggtaa accgcaggtc tgcgattttc gaggcaggga atctgcgcgc gcgcgcgggc
     1141 gatctgttcc aggttgatat cggctatcac cacgcccggc atgtcctgcg cctgtgccag
     1201 tatcaatccc cagggatcga caatcatcga gcgtccgaaa cattgccggt tgggagggta
     1261 cgttcccacg tgagcgggcg cgacgacata gcacaggttc tcgatggcgc gcgcgcgcag
     1321 caggacttcc cagtgatcgc gcccggtatg caaggtgaac gcggctggca cgaagaggat
     1381 gtccgccccg cgcagggtca gcaggcggta caattcgggg aagcgcaggt catagcagat
     1441 agtcagaccg aaggtaatgc catcgatctc ggcggtcaca agctcgtcgc ccggctcgat
     1501 gcga
//
LOCUS       DASZAK010000014         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_319712, whole genome shotgun sequence.
ACCESSION   DASZAK010000014 DASZAK010000000
VERSION     DASZAK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319712"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VGT44_00190"
     CDS             <1..361
                     /locus_tag="VGT44_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579240.1"
                     /translation="GAELPLDATSIMRIFYATKHQLDASGSTIPEVHRDIIVLGASAY
                     AMEAYQSPTNDNFDFQDGALRDRIDDTKIPAAWLAAAKNKMQQFEARLLEIKQQRDFA
                     ASSRAQWGDIPARWPRL"
     gene            417..788
                     /locus_tag="VGT44_00195"
     CDS             417..788
                     /locus_tag="VGT44_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579241.1"
                     /translation="MLNFLSVLNLVVTLSLMVGGLLAYQHGFTRTANEVQERVIHLLQ
                     SEIGALQDRISALEKENTRLNHIITTIRSSLKRRGLYITIDGELVSIHERGGRMTQAT
                     RIHGEAAARDAARDDEEEEEG"
     gene            790..1230
                     /locus_tag="VGT44_00200"
     CDS             790..1230
                     /locus_tag="VGT44_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579242.1"
                     /translation="MPTIGQDCEVVLDGTGYFVKPGTYKMKQPRIRKATVRADGGESY
                     VDLGPGKRVWSMVMLCLNDLVKYDGTSIGLTGQQYRDALRSSYVNSTGVVISFTDAQN
                     GTALSVHFDSYAEMIADLHVQQVALSTASAPALTYEVEIELVEA"
     gene            1340..>2049
                     /locus_tag="VGT44_00205"
     CDS             1340..>2049
                     /locus_tag="VGT44_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579243.1"
                     /translation="MHYNTNRTQSMSISPSRALGVVTVMVFCLAMYLVSTRMTVPQVH
                     ASANVPPGTISSSPASGPVGTTITVSGSGWSQPDGTPVYFGYLVNSTCSLVSDSQNGS
                     LSGGAFSGWMRWPLGTPANVYTVCAIISGDSQTLDAGSFTVLSTSAPAVSITPASLQE
                     NQQATITATNFVPPGSSINFFWTLTNGSIVQSLGSAISNGVGTALLTFTVPIMPLNSG
                     SYLIQSQSGAGQPPPYSAS"
BASE COUNT          432 a          597 c          600 g          420 t
ORIGIN      
        1 tggagcggag ctgcccctgg atgccacctc gatcatgcgc atcttttacg ccaccaaaca
       61 ccagcttgat gccagcggct caaccatccc cgaagttcac cgcgatatca tcgtgcttgg
      121 ggccagcgcc tacgccatgg aagcctacca gtcaccgacc aacgacaatt tcgatttcca
      181 ggatggcgcg ctgcgcgacc gcatcgacga tacgaagatc ccggcagcat ggctggccgc
      241 cgcgaagaat aagatgcagc agttcgaggc gcgcctgctg gagatcaagc agcagcgcga
      301 ctttgccgcc tcgtcccgcg cccagtgggg cgatatcccg gcccggtggc ccaggctgta
      361 ggatagcacc cggcagtgag ggaggctgta ttccagcata taaaaggaga attccaatgc
      421 tcaattttct cagcgtactc aacctggtgg tcacgctcag cttgatggtg ggcgggttgc
      481 tggcgtacca gcatggcttc acgcgcacgg cgaacgaggt gcaggagcgc gtcatacatt
      541 tgctgcaaag cgagatcggg gcgctgcaag accgcatctc ggcgcttgaa aaggagaata
      601 cgcgcctcaa tcacattatt acgacgattc gctcttcgct gaaacggcgc gggctgtata
      661 tcacgatcga tggcgaactg gtgagcatcc acgaacgcgg cgggcggatg acgcaggcca
      721 cgcgcataca tggcgaggcg gcggccaggg acgcggcgcg cgacgacgag gaagaggagg
      781 agggatagta tgccgacgat tgggcaggat tgcgaggtgg tgctggatgg caccggctac
      841 ttcgtgaagc cggggacgta taagatgaag cagccgcgca tccgcaaggc aaccgtgcgg
      901 gccgatgggg gcgagtcgta tgtggacctg gggccgggca agcgtgtgtg gagcatggtg
      961 atgctgtgcc tcaacgacct ggtgaaatac gacggcactt caatcggcct gacggggcag
     1021 caatatcgcg atgcactacg gagcagttac gtcaattcga ccggcgtggt gatctccttc
     1081 accgacgcgc aaaacggcac ggcgttgagc gtccactttg atagctacgc cgagatgatc
     1141 gccgatctgc atgtccaaca agttgcgctt tctaccgcaa gtgcgcctgc tcttacatat
     1201 gaggttgaga tagagctggt agaagcataa aaagtatagt acgatttacc ctcttgcttg
     1261 acagctctgc cttacgacct ctacactccc atcagtaggg ggaatgtgcg cttcatatta
     1321 ttatggggga agtgtcacta tgcattacaa tacaaaccgc actcaaagca tgtctatctc
     1381 gccgtcacgc gcgctgggcg tcgtaacggt gatggtgttc tgcctggcga tgtatttggt
     1441 ttcaacgcgt atgaccgtcc cacaggttca tgcatcagca aatgttcctc cgggtacaat
     1501 cagcagttct cccgcatccg gcccggtggg aacgaccatc accgtgagcg gctccggctg
     1561 gtcgcagccc gatgggacgc cggtctactt cggctacctg gtcaattcca cctgttctct
     1621 cgtcagcgac tcgcagaatg gctcattgag cgggggcgcg tttagtggct ggatgcgctg
     1681 gccacttggc accccggcta acgtgtatac cgtctgcgct atcatcagcg gcgatagcca
     1741 gacgctggat gccggatcgt tcacggtgct ttcgacatca gcgcctgccg tctccatcac
     1801 acctgcttcg ctgcaagaaa accagcaggc aaccatcacg gctaccaact ttgttccgcc
     1861 aggctcttcg atcaactttt tctggaccct gaccaatggg agcattgtac aatcgcttgg
     1921 cagtgctatt tcaaacggtg tcggaaccgc gctgcttacc ttcactgtgc caattatgcc
     1981 cctcaatagt ggctcctacc tgatccagtc ccagtccggc gcgggccaac cgccgccgta
     2041 ttccgcgtc
//
LOCUS       DASZAK010000015         3087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_674684, whole genome shotgun sequence.
ACCESSION   DASZAK010000015 DASZAK010000000
VERSION     DASZAK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3087
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674684"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..669)
                     /locus_tag="VGT44_00210"
     CDS             complement(<1..669)
                     /locus_tag="VGT44_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase H family protein"
                     /protein_id="HEV2579244.1"
                     /translation="MSLWNDNQTSSDPLEWRSVLNALQHIPVESARATLGTHGTVAYT
                     DGACIKNPGGPAGWSVILVSADYVDGERIREDAPRIESYGHIPPSENTTNNRAEIAAV
                     LAALSLAPRDQSLTIHSDSEYTIKVAQGVYKMKANSDLWDYYRLLLKKRTVAPTFVWV
                     RGHAGQEHNERADELAGLGAFNNDRQAYLRWQASTSPEARSGLLSPELAALRQHVQRL
                     QTYFD"
     gene            complement(750..2312)
                     /locus_tag="VGT44_00215"
     CDS             complement(750..2312)
                     /locus_tag="VGT44_00215"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV2579245.1"
                     /translation="MKAKSACLIINPRSGENLAKLNDVLAVLSAAGWDADVFIKEYGG
                     HTLELAKNAGKQKYDLVIAYGGDGTLNQVVNGVMYTNKKSSSVGLLPGGTANEWANEV
                     GIPVDPVKSALSLINSRARRVDVGHITVEGLTFPPTAGESTSDTSSTPAASSNGAPSL
                     KKRKTTTNARQDFLMMAGLGIDAAVMGHVSKPLKYHIGPIAVGLSAAYELPKQHPFPV
                     EIRVTASNGEEGQAWKGEALQVVVSNTRKYADVIEMSPDAYIDDGSLDVTVITASDSI
                     TTMQQIMSLVFRRKPDSLTAEVLHGSSLHISAPASIAMQLDGTAVKLQDYLSKKDRTA
                     LKDAIDPSQVMVTYRFDAVPRSLSVAIPCSYNDALFESAEDQSNDHANGAQQADTKPT
                     NAAGQEEQAQTSQVGEQADQEQGEQAGQSDPLAIPAPLKRGRKVRVIGASHNPDKQKP
                     LYVIAGSAIKSSTGEFSPVAVRVNGKTTVLHRTGGAAPLDDIEKLPEGVEIAVEGKKS
                     KRGVIVARQVVI"
     gene            complement(2419..2988)
                     /locus_tag="VGT44_00220"
     CDS             complement(2419..2988)
                     /locus_tag="VGT44_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579246.1"
                     /translation="MKETLSQRADEDAAQEQASPQGIGMAVAFDWGLSVQILATPVLT
                     GVFGFSSPIQGIDVSSTLARVLLFVASLPFAYLLAFFGNAVRRGRDWARRIQLVANIL
                     LTLVGIAGIINLYRGIKAGNFWPLVTEVILLIFSPLIAWRLSRPVTARWFKSVTSADA
                     SKRHGGLWIFFIALWAIAGGILQAIAGLK"
BASE COUNT          634 a          899 c          850 g          704 t
ORIGIN      
        1 gtcgaagtag gtttgcagac gctgcacgtg ctggcgcagc gcggccaact caggcgaaag
       61 caggccgcta cgcgcttcgg gcgaggtgga cgcctgccag cgaagatacg cctgtctatc
      121 gttgttgaac gcgcccagcc ctgccagctc atcggcgcgc tcgttatgtt cctggccagc
      181 gtggccgcgc acccagacga aggtgggcgc aaccgtgcgc tttttgagca gcaggcggta
      241 atagtcccac aggtcgctgt tggccttcat tttgtagacg ccctgggcaa ctttgatagt
      301 gtattcgcta tcgctgtgaa tagtcagcga ttgatcgcgt ggggccagcg agagcgcggc
      361 cagcacagcg gcaatctccg cgcgattatt ggtggtgttc tccgatggcg ggatatgacc
      421 ataactttcg atgcgcgggg cgtcctcgcg gatgcgctcg ccatcgacat aatcggctga
      481 gacgagaatg acgctccagc cagccggccc gcctggattc ttgatacacg cgccatcagt
      541 gtaagcgacg gttccatgcg ttcccaacgt cgcccgcgcg gattcgaccg ggatgtgttg
      601 caaagcgttt aagaccgaac gccactctaa cgggtcgctg gatgtttgat tatcgttcca
      661 caggctcacg cgatatatcc tctttctcgt tatgcttgtt tcacctggcg acgcctattg
      721 tatcacgcat aacgagattt gaggaggagc tagatcacaa cctgcctggc gacaatcact
      781 ccgcgtttgc tctttttccc ctcgacggca atttctacgc cctccggcag cttttcaata
      841 tcatcaagcg gcgccgcgcc tcccgtgcgg tgaagcacgg ttgtcttacc attgacgcgc
      901 acggcgaccg ggctgaattc tcccgtcgat gatttaatgg cgcttcccgc gatgacgtac
      961 aacggcttct gcttatcagg attgtggctc gctccaatga ctctgacctt tcgcccccgt
     1021 tttaacggcg cgggtatcgc cagtggatca ctttgcccgg cctgctctcc ctgctcttga
     1081 tcggcctgtt cacccacctg gctcgtctgt gcttgttctt cctgccccgc tgcattcgtc
     1141 ggctttgtgt cggcctgttg cgcaccgttc gcgtgatcgt tcgattgatc ctctgctgac
     1201 tcaaacaagg catcattgta actgcaaggg atcgcaactg acagcgagcg cggcaccgca
     1261 tcaaaacgat acgtcaccat gacctggctg ggatcgatgg catccttcag agccgtgcgg
     1321 tccttcttgc tcaggtaatc ctgtagcttg acggcggtgc catccagctg catggcaatc
     1381 gatgccggcg cgctgatatg cagactcgaa ccgtgtaaga cttcggcggt aagcgaatct
     1441 ggtttacggc gaaacaccag cgacataatt tgttgcatgg tcgtgatgga atcgctggcg
     1501 gtgatgaccg tgacatccaa cgagccatcg tcgatatagg catctggcga catctcaatg
     1561 acatcagcat atttgcgcgt attgcttacc acaacctgca gcgcttcccc tttccacgcc
     1621 tggccttctt cgccgtttga agctgtaacg cgaatctcca ccgggaaagg atgctgtttg
     1681 ggcaattcat acgcggctga aaggccgacg gcaatggggc cgatgtgata tttgagcggc
     1741 ttagaaacat gacccataac agcggcatca atgccgaggc ccgccatcat caaaaaatcc
     1801 tggcgggcat tcgtagtcgt tttgcgtttt ttgagagacg gtgcgccatt actggaggca
     1861 gccggtgtac tcgatgtatc cgaggtagat tcacccgcgg tcggcggaaa ggttaaccct
     1921 tctacagtga tatggccaac atcgacgcga cgcgcacggc tgttgataag ggagagcgcc
     1981 gatttcaccg ggtcaactgg tatgccaact tcatttgccc attcgttggc ggtgccgccc
     2041 ggcagcaacc ccacactgct gctcttcttg ttcgtataca tcacgccgtt gacgacctgg
     2101 ttgagcgttc catctcctcc ataggcgata acgaggtcat atttctgttt accggcattt
     2161 tttgccagct ctagggtatg gccgccatat tccttgatga atacatcggc atcccaaccc
     2221 gctgctgaca acaccgccag cacgtcattc aactttgcca ggttttcccc cgacctggga
     2281 ttgataatga ggcaggcgct cttcgctttc atctatcgtt actccttcct ggtatctgat
     2341 gcaattaact tttgctatat cgtcagtcac gtacagcaca gtaacgacgt ttcatgctcc
     2401 aggaaagagc caggattgtc atttcagccc ggcaatagct tgcaaaatgc caccggcgat
     2461 agcccacagc gctataaaga agatccacag gccgccatgc cgtttgctcg cgtcggcgga
     2521 tgtaaccgac ttgaaccagc gcgctgtcac cgggcggctc aatcgccagg caatcagcgg
     2581 cgagaagatc aacaagatca cctcggtgac gagcggccag aaattgcccg ctttgatgcc
     2641 gcgatacagg ttgataatac ctgcaatgcc taccagggta agcaggatat tggcgacaag
     2701 ctgtatcctg cgcgcccaat cgcgtccgcg cctcaccgcg ttgccaaaga aggccagcag
     2761 gtaagcaaag ggcagcgatg cgacgaaaag caacaccctg gccagcgtcg aactcacatc
     2821 aatgccctgg atgggagatg aaaaaccgaa tactccggta aggacagggg tcgccagaat
     2881 ctgtaccgac aggccccagt caaaggcgac ggccatgccg atgccctgcg gagatgcctg
     2941 ctcctgcgcg gcatcctcat cagcccgctg tgaaagcgtt tctttcaagg aagggggtct
     3001 ccatcttcta aaatgttttg cgcccgtagg ggcgggggag gacgatgggt ggagagggga
     3061 cgcgtgcggc gcccgctgag cctgttg
//
LOCUS       DASZAK010000016         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_213315, whole genome shotgun sequence.
ACCESSION   DASZAK010000016 DASZAK010000000
VERSION     DASZAK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213315"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VGT44_00225"
     CDS             <1..598
                     /locus_tag="VGT44_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2579247.1"
                     /translation="LPVPPRQLASSHAYPTRPLSDPVTPPPIFAANQPPVFPQSLLPE
                     HSTPPPLPPALSQLSSSFEHEAYLPPLLPGVCIIRSGALVGNSFPLDRPHLIVGRMAG
                     CDVRIDDPALTIEYAQFVCSPEGDSVYGVGVLVNDMPMQSPRLLQAGDLIWLGAVCLE
                     YAPVTEAKTIPLSPPAMPSSSARTASSPMHLRLPSKPK"
     gene            complement(601..1383)
                     /locus_tag="VGT44_00230"
     CDS             complement(601..1383)
                     /locus_tag="VGT44_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2579248.1"
                     /translation="MDTAIESKEQPQPVAQRPRQVHTITDRFSMANTYVIDDGQLVVV
                     DPGSELNVQQLLTYLRDFLRRDPGEIDLVALTHLHPDHTAGLRMLRQASRAPVAASIV
                     ARWLAQSDTDETQREGRNLAEIVSGTSVFVGNMFSQVRLSGTLHRLDNVSSNYQRQAE
                     LVDLWIEDVHGLPGHPDWRVIASPGHTPDSLCFYNPFSYELICGDTLITLDGGAILVR
                     GGTNRHKLQQTLQTLQSLKVYYLYPGHGRPILAQRALARIEW"
     gene            1651..>3604
                     /locus_tag="VGT44_00235"
     CDS             1651..>3604
                     /locus_tag="VGT44_00235"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2579249.1"
                     /translation="MTERCSYIIIGNGIAGITAAETLRNEDAAAEITVIGEHPMPVYT
                     RPALKDFLAGRSSEEDLYARRRSFFADHRLHFVFDRAVSVEVDAHSVQLRSGRHMSYD
                     RLLLATGARARRLSCPGCDLAGVVALRTLADFQSARQSLEAARSVVVVGAGPVALETV
                     EILHHRGYQVTHLLRGRRLWPEVLDMTASNLVLQQERREGIDVRAEEEVMEIVGRQGG
                     VVGVITRSGARIACDLVITAIGSEPVIDFIQASGIACARGVKADHAMRTAAPDIFAAG
                     DVAEVIDPITGQPRCISQWYPAIQQGRTAAYAMLGHLVTHHLMQPSTHNNAYLRSITT
                     AYFFGYDIAAVGITTPNALIASRTPVVSNAYQEIFADPGTHLYSKALLKDGILIGALS
                     FDGRRDMLACKRAIDHAVSLTPVASHLFDREFKLANWLDSQKVPTPVLAVRKARIDNQ
                     RPNSVGKSTPAHGMKTQFASPPGVRVGTLVGATRLRAGGDPYTMPIVTVTMSGAPGGS
                     PASALAMSQEIAFLAPILPLETVRALYTAMPDKGSNHPYSLSLGGDTALSRPGEEADM
                     SSLAPEWSHTLLNPGETITIGRDSTSTLVLNHGTVSRHHAEITCDHGYYCLRDLDSKN
                     GTFVNGARLEPYRVHVLQPRDQVRIGT"
BASE COUNT          693 a         1156 c         1012 g          743 t
ORIGIN      
        1 ccttccggtt cctccccggc aactggccag ttcccacgcc tacccgacgc gccctctctc
       61 cgacccggtg acgccgcctc ccatatttgc cgcgaatcag ccgcccgtgt ttcctcagtc
      121 gctgctgcca gaacattcaa cgccgccgcc cctgccaccg gcgctatcac agctatcttc
      181 atcgttcgag catgaagcgt atctgccgcc attgctgccg ggagtgtgca tcatccgttc
      241 cggcgcgctg gttggcaata gcttcccgct cgaccgcccg cacctcatcg ttggacgcat
      301 ggcaggctgt gacgtgcgca tcgatgatcc tgccttgacc atcgaatacg cgcaattcgt
      361 atgctcgcca gaaggcgata gcgtctacgg cgttggtgtg cttgtgaatg atatgccgat
      421 gcagtcgccg cgcctgttgc aggcgggtga tctgatctgg cttggcgctg tttgcctcga
      481 atatgcgccc gtcacagaag cgaagacgat ccctctctcg ccgccggcca tgccttcatc
      541 gtctgctcgc accgccagca gccccatgca tctacgcctg cctagcaaac ccaaataatg
      601 ctaccactca attcgcgcca gggcgcgctg tgccaggatg ggtcggccat gtccaggata
      661 gaggtaatag accttcaagc tctgtagcgt ttgcagggtt tgctggagct tgtggcggtt
      721 ggtgccaccg cgcaccagaa tggcgccacc gtcgagtgtg atgagggtat cgccgcagat
      781 caattcgtag ctgaatgggt tgtagaagca taggctatcg ggcgtatgac cagggctggc
      841 gatcactcgc caatcgggat gcccaggcag accatgcaca tcttcaatcc agagatccac
      901 cagctccgcc tggcgctgat aattcgagga gacattgtcg aggcgatgca gcgttcccga
      961 caggcgcacc tggctgaaca tattcccgac gaataccgat gtcccactca ctatctcggc
     1021 gagattgcgt ccctctcgct gcgtttcgtc tgtatcggac tgcgccagcc agcgcgctac
     1081 aatcgacgcg gccaccggcg cgcgcgacgc ctggcgcagc atcctcaggc cggcagtatg
     1141 atcgggatgc aggtgcgtca gcgcaaccag gtcaatctcg cccggatcgc ggcgcagaaa
     1201 atcacgcagg taggtcaata attgctggac gtttagctca gagccaggat caaccaccac
     1261 cagttgccca tcatcaatta cataggtgtt agccatcgag aagcgatcgg taatcgtatg
     1321 tacctgacga ggccgctggg ccaccggctg cggctgctct ttactttcaa tcgcagtgtc
     1381 catgctgacc tatcctctgc tacaacaaag ttccgaaagg acatgccctt gcggtcgccc
     1441 ttccgaccct acttctctcc tgattatagc atagtacgcc tccggtgagc cggtttctca
     1501 aagggcgtac ccttgcggcc gccctttgag aatgggtaat ctgtaccaca tttacccatt
     1561 ttcacgctat tttaacagga attgtttaca ctggcataga gatacatata ttgacacacc
     1621 ataatatata acggggaaca ggggtgtact atgactgagc gttgctcgta catcatcatc
     1681 ggcaatggga tcgccggaat cacggcggcg gagacgctgc gcaacgaaga tgcggcggcg
     1741 gagatcaccg tgattggcga gcatcccatg ccggtctata cccgcccagc attgaaagac
     1801 ttcctggcgg gccggtcgag cgaggaagac ctgtacgcgc gccgccgcag cttcttcgca
     1861 gatcaccgtc tccacttcgt ttttgaccgt gcggtgagcg tcgaggtgga tgcccatagc
     1921 gtccagttgc gcagtggacg gcatatgagc tatgatcgct tgctgctcgc gacgggcgcg
     1981 cgcgcccgtc gcctgtcctg ccctggatgt gacctggcgg gcgtcgtggc gctgcgcacc
     2041 ctcgccgatt tccagtcggc gcggcagtcc ctcgaggcgg ctcgcagcgt ggttgtggtg
     2101 ggcgctggcc ccgttgccct tgaaaccgta gagatattgc atcatcgcgg ctaccaggtg
     2161 acgcacctgc tgcgtggccg caggctctgg cccgaagtgc tggatatgac cgcctcgaac
     2221 ctggtcttgc agcaggagcg gcgcgaaggc attgatgtac gcgccgagga agaggtcatg
     2281 gagatcgtgg gcaggcaggg tggtgtggtg ggggtcatca cgcgcagcgg cgcacgcatc
     2341 gcctgcgacc tggtcatcac agctatcggg tctgaaccgg tcatcgattt catccaggcc
     2401 agcggcatcg cctgtgcgcg cggcgtcaag gccgatcatg ccatgcgcac agccgcgccc
     2461 gatatctttg cggctggtga tgtcgccgaa gtcatcgatc ccatcaccgg ccagccgcgc
     2521 tgcatcagcc agtggtaccc cgccatccag cagggcagaa cggcggccta cgccatgctt
     2581 ggccatctcg tcacgcatca cctcatgcag ccttctaccc acaacaacgc ctatttacgc
     2641 tcgatcacga cagcctattt ctttggctac gatatcgcgg cggtcggcat caccaccccg
     2701 aacgcgctga ttgcgtccag gactcccgtt gtatccaatg cctaccagga gatctttgcc
     2761 gatccgggta cgcatcttta cagcaaggcg ttgctcaagg acggcatcct gattggcgcg
     2821 ctctccttcg atggccgccg tgacatgctg gcctgcaaac gtgccatcga ccacgccgtt
     2881 tcgctgaccc cggtcgcatc acacctgttc gacagagaat tcaagcttgc gaactggctc
     2941 gacagccaga aagttccaac ccccgtgctg gccgtgcgca aggcgcgcat cgacaatcag
     3001 cgtcccaata gcgtcggcaa atcgacgccg gcgcacggca tgaaaacaca gttcgcatcc
     3061 cctcccggtg tccgcgttgg tacgctcgtt ggggcaacac gcttgcgtgc cggtggcgac
     3121 ccttacacca tgcctatcgt gaccgttacc atgtcaggcg cgcccggcgg ttccccagcg
     3181 tcggcattgg cgatgagcca ggagatagct ttccttgctc ctatactccc gttggagacc
     3241 gtcagagcct tgtatactgc catgccggac aagggaagta atcatcccta cagtctttcg
     3301 ttaggcggcg atacagcgct aagcaggcca ggtgaagagg cggacatgtc ctcgctcgcg
     3361 cctgagtggt cgcacacgct cctcaatcct ggcgaaacaa tcacgattgg gcgcgactcc
     3421 acctcaacac ttgtcctcaa tcatggcacc gtttcgcgcc accacgcaga aatcacctgc
     3481 gatcatggct attattgcct tcgcgacctg gacagcaaga atggcacgtt cgtcaatggc
     3541 gcgcggctcg aaccgtaccg cgtccacgtg ctgcagccac gcgaccaggt acgcatcggc
     3601 accc
//
LOCUS       DASZAK010000017         3917 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_71278, whole genome shotgun sequence.
ACCESSION   DASZAK010000017 DASZAK010000000
VERSION     DASZAK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3917
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71278"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1166)
                     /locus_tag="VGT44_00240"
     CDS             complement(54..1166)
                     /locus_tag="VGT44_00240"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579250.1"
                     /translation="MRTLDSSLRVIWACLKKDLKSARAEPLYTLVSIILPLNVLLLMS
                     LLVISGGLAPTAVVMQDTGPLAQQFYTAMSQAHSFVLQQSTAQQANALIEQGKIVAIV
                     TVPADFDARIRQRQSVTVGVQINNLNTDFTNDIRRALPLSITSFYGRAEPSVVTITPQ
                     ERDTYPQDLGYVPFLAVPILVIGLMVGGMVQAGTAAAVEWEKETMKELLLSPASRLAI
                     LLGKMLAAFLTGLTGAAIVLAVLIAVMGIWPVHWGELIGFTLLILTLFTAWGTLLGTW
                     LKRRTPLATLAIGLSVPMFFLSGPFGPISFFAPIEQLVARVFPVYYAIVVLQHAVHDF
                     TLNTSGIGINALILVAYALGGLVLATIVVRRSTLAH"
     gene            complement(1179..2162)
                     /locus_tag="VGT44_00245"
     CDS             complement(1179..2162)
                     /locus_tag="VGT44_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2579251.1"
                     /translation="MRNKGKQEATAPHLTGTTEDPAIALRALCKSFGTLRALDHLTLS
                     VGQGEIFGLLGPNGSGKTTTINLISGLATPTGGEVQVLGYEMPRHARRIRQMLGAVPQ
                     ETALYEELSAWDNLDFHADLFGMARKEKKARIMQMLDLVQLQERMHSRVNTFSGGMKR
                     RLALARALLHDPRLLYLDEPTLGVDVQSRRAIWDYILALRDQGKTVLITTNYLEEAQA
                     LCERIAILDHGKLIAVDTPGHLKQTYGGSVVELETAQPTTAADDIRRLPGVQEVVQVG
                     TSMRVTTQGGNDALPQIITLVTLHGELRQIAVREPTLDEIFLRLTGTALRD"
     gene            complement(2275..2718)
                     /locus_tag="VGT44_00250"
     CDS             complement(2275..2718)
                     /locus_tag="VGT44_00250"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV2579252.1"
                     /translation="MSPIVLTIEIARPPDEVFSYVTDPSRFAEWQEDAVRGRREGDGP
                     PGLGSRFTTTRRIGRAERTTTSEITEITPPRSWAAHGVDGPIRPHMHVTVAPLGDRAR
                     SRVTIEVAFEGHGIGKLLVPLVRWLGRKDSPTNCRNLKEQLESGD"
     gene            complement(2811..3275)
                     /locus_tag="VGT44_00255"
     CDS             complement(2811..3275)
                     /locus_tag="VGT44_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579253.1"
                     /translation="MNLVTSLIALATAINGLLSGLNLDTALVKLPARRRIGAVAYATF
                     ARGNDLGNGLVVYPLLGVGAALLTVLATALAYVQLSSMEVLVLLSLASLLSLLHSFAT
                     TRAAPVMLSLKEAPDDETILAAQLDRFARWHTVRATFQVLAFFFLLWVLASV"
     gene            complement(3275..3829)
                     /locus_tag="VGT44_00260"
     CDS             complement(3275..3829)
                     /locus_tag="VGT44_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579254.1"
                     /translation="MIDGFPLSFFLPLVVHALAAMTTAVAGVITFSRPKRRGRHPKWG
                     VRYLWAYTVVFLTAIILSVQHWPTDAYLVVLATIGYGFALGGYVARRFRRKPWLLRVL
                     GKQWISVHIVGMIGSYVVLWTAFYVDNAHLIPGLNQLQTLIFWVLPTVIALPFLVVSL
                     SRFAPKVTIPAKFESASNRKERTS"
BASE COUNT          812 a         1144 c         1188 g          773 t
ORIGIN      
        1 gtccataccg cgcgcacaaa ggctcgtagc atcttcattc tctcctctct ttgctaatgt
       61 gccagagtgc tgcgccgcac gacaatagtt gccagtacca ggccgcccag cgcataagcg
      121 accaggatca gcgcgttgat accgatccca gaggtgttca gggtaaaatc atggacggcg
      181 tgctgcagca ccacgatggc gtaatagacg ggaaacacgc gggcgaccag ctgctcgatg
      241 ggcgcgaaaa aggagattgg cccaaaagga ccgctcagaa agaacatcgg aacgctcagg
      301 ccgattgcga gggtcgccaa tggggtgcgc cgctttaacc acgttccgag cagtgttccc
      361 caggcagtga agagcgtgag aatgagcagg gtgaagccga tcaactcacc ccaatgcact
      421 ggccagatcc ccatcaccgc gatcaggacc gcgagtacga tcgccgctcc agtcaggccg
      481 gtcaagaacg ctgccaacat cttgcccaac aggattgcca gccgactggc tggggacagc
      541 agcagttctt tcatagtctc cttttcccac tccaccgccg ctgctgttcc cgcttgcacc
      601 atgcccccga ccatcaggcc gatcaccagg atgggaaccg ccagaaaggg gacgtagccg
      661 agatcctgcg ggtaggtgtc gcgctcctgc ggcgtgatgg tcacaacaga gggctcggct
      721 ctgccgtaga aactggtgat cgagagcggc agcgcgcggc gaatgtcgtt ggtgaaatca
      781 gtattgaggt tgttgatctg cacccccacc gtgacgctct ggcgctggcg gatacgcgca
      841 tcaaagtctg ctggaacggt gacgatggcc acgatcttgc cctgctcgat caacgcattg
      901 gcctgctgtg cggtggattg ctgtaacaca aacgaatggg cctggctcat ggctgtgtag
      961 aactgctggg caaggggtcc ggtatcctgc atgaccactg ccgtgggcgc cagtccgccg
     1021 ctgatcacca gcaggctcat cagcaacagc acgttcagcg gcagaatgat cgagaccagc
     1081 gtatagagcg gctcggcgcg cgcgcttttg aggtccttct tcagacaggc ccagatgacg
     1141 cgcagggaag aatcgagtgt gcgcatgatg cttgcctcct aatcgcgtaa cgccgttccc
     1201 gtcaaacgca gaaaaatctc gtccagggtg ggttcacgta cggcgatctg gcgtagttcc
     1261 ccgtgcagag tcaccagggt gatgatctgt ggcaaggcat cattgcctcc ctgggtggtc
     1321 acgcgcatgg atgtgcctac ctggacgact tcctggacac ctggaagtcg gcgaatgtcg
     1381 tcggcggcgg ttgtcggctg cgccgtttcc agttccacga cactcccgcc gtaggtctgc
     1441 ttgaggtgtc cgggtgtgtc gacggcgatg agcttgccat gatcgaggat ggcgatgcgc
     1501 tcgcagagcg cctgcgcctc ttccaggtag ttggtggtaa tgagtaccgt cttgccttga
     1561 tcgcgcaggg cgaggatata gtcccagatg gcgcggcgcg attgcacgtc cacgcccagc
     1621 gtaggctcat caaggtataa gaggcgcgga tcatgcagca gcgcccgcgc cagggcgagc
     1681 ctgcgcttca tcccgccgga gaaggtattc acacgcgaat gcatgcgctc ctgcagttgg
     1741 accaggtcga gcatctgcat gatgcgcgcc tttttctctt tgcgtgccat gccaaagaga
     1801 tccgcatgaa agtccaggtt gtcccaggca gagagttcct cgtagagcgc ggtctcctgg
     1861 ggtacggctc ccagcatctg ccgtatccgg cgcgcatggc gcggcatctc gtagcccaac
     1921 acctgcacct cgcctccggt cggggtggcg aggccgctga tcaggttgat ggtggtggtc
     1981 ttgcccgagc cgttgggtcc cagcaggcca aagatctcgc cctgcccgac cgagagggtg
     2041 aggtggtcaa gggcgcgcag tgtcccgaat gatttgcaga gcgcgcgtag cgcgatagct
     2101 ggatcttccg tagttcctgt gagatggggt gctgtcgctt cttgctttcc cttgttacgc
     2161 atagacctct ctctttctgt atgcacgtat gtgcttttct cagggcttgc gaagctcgtt
     2221 tcgagcaagc tcgtgtggct cgatcgcatc gaggtgtact cagcacgagg cgcgctaatc
     2281 gccgctctcg agctgttcct tgaggttccg acagttcgtg ggactgtcct tgcgtcccag
     2341 ccagcggaca aggggcacga gcaacttgcc gatcccgtgg ccctcgaagg cgacctcgat
     2401 cgtgacgcgc gaccgcgcgc ggtcgcccag aggtgccacg gtgacgtgca tgtgtggcct
     2461 gatcggtccg tcgacaccat gagcggccca acttctggga ggagtgatct ccgtgatctc
     2521 cgacgtcgtt gtccgctcgg cgcgaccgat ccggcgagtg gtggtgaacc ttgagcccaa
     2581 gccgggcggg ccgtctccct ctctgcgtcc tctcacggca tcttcctgcc attcggcgaa
     2641 ccgcgagggg tcggtgacat aggagaacac ctcgtctggc ggacgggcga tctcgatggt
     2701 caacacgatt ggtgacatgg ttcctcctcc ttttgattga tcgatgattg ctccttccag
     2761 agcaactaga acaaaaacct cttttctcac atatcgtggg ctgatccaca tcacacgctt
     2821 gccaacaccc acagcagaaa aaaaaacgcc agcacctgga acgtcgcccg aacagtgtgc
     2881 cagcgtgcga agcggtcaag ttgtgcggcc aaaatggttt cgtcatcagg tgcctctttg
     2941 aggctgagca tcaccggtgc tgccctggtg gtggcaaacg aatgcaggag cgagagcagc
     3001 gaggccaggg aaaggagtac caacacctcc atcgaggaga gctgcacata ggctaaggct
     3061 gtggcaagca ccgtaagcag ggccgcacct acccccagaa gcgggtagac caccagccca
     3121 ttccccagat cattgccacg tgcaaaggtg gcatacgcca cagcaccaat gcgtcggcgt
     3181 gctggcaatt tcacgagcgc agtatccagg ttgagtcctg aaagcaggcc attgatcgcg
     3241 gtcgcgaggg caatcaagga tgtaaccaga ttcatcagct tgttctctcc ttccggttcg
     3301 atgcactctc gaatttcgca ggtatggtga ctttcggtgc gaaacgggag agggacacca
     3361 ccaggaacgg gagcgcgatc acggtgggca gtacccagaa tatgagtgtc tgaagctgat
     3421 tcaagcctgg gatcaggtga gcgttatcca cgtagaaggc cgtccacagg acgacgtagg
     3481 acccgatcat gccgacgata tggacactaa tccactgttt ccccagcacg cgcagcagcc
     3541 agggtttccg ccgaaagcgt cgcgccacat agccaccaag tgcgaagcca tagccgattg
     3601 ttgccagaac gacgaggtag gcgtccgttg gccagtgctg gaccgagagg atgatagccg
     3661 tgagaaacac cacggtgtag gcccacagat agcgtactcc ccacttcgga tgacgcccgc
     3721 ggcgtttggg cctgctgaac gtgatcacgc cggccactgc tgtcgtcatc gccgccaacg
     3781 catgcacgac gagcggcagg aagaaagaga gaggaaagcc atcaatcatg cgctaactcc
     3841 ttcgaaccta tcgatttctt gaagcgtttg cttctaataa ggagtgtagc tgcatgttgg
     3901 caagatgaga agtgaaa
//
LOCUS       DASZAK010000018         5485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_674754, whole genome shotgun sequence.
ACCESSION   DASZAK010000018 DASZAK010000000
VERSION     DASZAK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674754"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..267)
                     /locus_tag="VGT44_00265"
     CDS             complement(13..267)
                     /locus_tag="VGT44_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579255.1"
                     /translation="MEPLDSDLEYIARGPRSATPGSRLNFLLIFPILGFLLILTFIGA
                     AIFQWDISDLIDSLVGLLLLLFVAFIGMMFWALAPRTNES"
     gene            complement(400..627)
                     /locus_tag="VGT44_00270"
     CDS             complement(400..627)
                     /locus_tag="VGT44_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579256.1"
                     /translation="MTDRTPTFGDLCSAIADGSLTATLNGSMYEVNALELRRYLGKFF
                     TAQSFSFTDSLLAPSGGDAQEWSASTHPSVV"
     gene            complement(684..1250)
                     /gene="rimM"
                     /locus_tag="VGT44_00275"
     CDS             complement(684..1250)
                     /gene="rimM"
                     /locus_tag="VGT44_00275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV2579257.1"
                     /translation="MSEQQQTEWATIGKVVALFGVRGELKVRLLTDVPNRFEQLDVIY
                     VGEERRSYPIESVRPYKGEMILLKLRGLRDANAAESLRNADLTIPLSRLAELPAGSYY
                     QHDIIGLRVFTLDETYLGTIVDIISTGSNDVYVVKKPDLSQVLIPAIKDVIKQIDLQK
                     HVMHIDPMPGLLDNSDRDLREEADAALE"
     gene            complement(1370..1600)
                     /locus_tag="VGT44_00280"
     CDS             complement(1370..1600)
                     /locus_tag="VGT44_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV2579258.1"
                     /translation="MRKLIEYVAKSLVDAPDAVQVREVRNDRNALVLELHVAPDDFGK
                     VIGRQGRVAKAMRTLLRVGGTREGRHTVLEIQ"
     gene            complement(1637..1927)
                     /gene="rpsP"
                     /locus_tag="VGT44_00285"
     CDS             complement(1637..1927)
                     /gene="rpsP"
                     /locus_tag="VGT44_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV2579259.1"
                     /translation="MTVKIRLKRVGKKKAPSYRVVVADSRSPRDGRFIENIGWYNPLV
                     EPSAIHIDEEKALGWLKNGAQPTDSVASLLKRAGITDRFEQMKAAAKPPAEA"
     gene            2186..2902
                     /locus_tag="VGT44_00290"
     CDS             2186..2902
                     /locus_tag="VGT44_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEV2579260.1"
                     /translation="MTTEQGGIVALVGSGEFLETTLPVDRYLVEQMQQRQLTPRVAIL
                     PTASAPDGGDVPQNWAKMGVEHFARLGLAAEPVMLLTRQDADNPAIVAQLAATNFVYF
                     SGGKPRYLMETLQKSAAWQTILDMFRQGGVVAGCSAGAMVLGGAFFDFPQIWSTKPAL
                     GLVPGIVVIPHFDEMPKLITSTIGGGKREGVVVGIEGGTALVVSHEHWIVQGKGGVTV
                     FNGKRRQRYQAGETILMKRI"
     gene            2946..3386
                     /gene="queF"
                     /locus_tag="VGT44_00295"
     CDS             2946..3386
                     /gene="queF"
                     /locus_tag="VGT44_00295"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEV2579261.1"
                     /translation="MNKEHPRTPGRYGTEEIEQSRLEAWPNSNPERDYITHLEIPEFT
                     CLCPRSGFPDFATIVIDYVPDQSLVELKSLKLYINQYRNRQISHEAATNTILDELVAL
                     LAPRWMRVVADFTVRGNIKTIIFAEHSAPGYTGPRPEYRRYMQG"
     gene            3405..4718
                     /locus_tag="VGT44_00300"
     CDS             3405..4718
                     /locus_tag="VGT44_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2579262.1"
                     /translation="MFCIYCGEHYPKEATFCPKCGRAVHTSLLLDSASSGSVPVTAGE
                     AAIIGSPSWRLDMHTSAKWIRRVGIPLAALAWLVLVAALLWAASHVARSLILLAIAAI
                     LAFALAPIVKLLQRFMPRLLAILLVYIVVMGGISIVLYFLIRAAIVQAEELKNQVSSL
                     LQPGQSSSLQALLASLGIDATQLQNVRQTITAHLGGLVGDIVPVLSGLFDAVLDVIIV
                     TMLSIYLLLDGSRVFRWLRENMPVPQQKRGYFLLDTLESVVGGYIRGQLTLSLLIGVL
                     VGVGMTIFRVPHPVLLGELAFLLAFIPVLGTFISGAFCILLSLTATNSWVVALTGQTW
                     ILAIIVLLYFVAMHFIESHIVGPRIVGHAVGLHPVVSIAALVASAELFGILGALFVVP
                     LVGMLQAIIVWLWRDWRAAHPDQFPGKPVTVESRVSGDEAQAAVE"
     gene            4779..5348
                     /locus_tag="VGT44_00305"
     CDS             4779..5348
                     /locus_tag="VGT44_00305"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEV2579263.1"
                     /translation="MAQREYSVMSVEDYLVLSRSSKDARYEYLDGDLRMLAGGSPDHS
                     IIIANLTATIKAPLKGGSCRVYNSDVQLRLSEKRYVFPDIAVSCDERDRNQKEMIRYP
                     RVVVEVLSPSTEAIDRGKKAAYYRACPTIQEYVMVDSIEVFIEVHRRTEELWTINTFE
                     AGDNVTLESLDIQFPREDAYEGTSLLNEP"
BASE COUNT         1253 a         1563 c         1495 g         1174 t
ORIGIN      
        1 ggctcgccga ggttaactct cgtttgtgcg gggagctaaa gcccagaaca tcattccgat
       61 aaaagcgacg aagagtaaca ggagcaggcc gaccaggcta tcgatcaggt cagaaatatc
      121 ccactgaaaa atggccgcgc cgataaaggt caggatcagc aaaaagccca ggatggggaa
      181 aatcaatagg aaattaaggc gcgaaccagg ggtggccgac cgaggcccac gagcgatata
      241 ttcaaggtcc gaatcaagtg gctccatacg aaacctttct acgtcagggt acaaagcgat
      301 gccgcctata ggctatatta taatcgctct gcgacttgaa cgcaacagca tcacagaggc
      361 gcgtcgatac aagggcaaaa aaaatcggcc agattcggcc taaacaacgg atgggtgagt
      421 cgatgcagac cattcctgcg catcgccacc tgatggcgct aatagcgaat cggtaaacga
      481 aaacgattgc gctgtaaaaa atttgcccag gtaacgacgc aattctaagg cgttgacctc
      541 gtacattgac ccgttgagag tagcagtaag gctgccgtca gcgattgcac tacacagatc
      601 tccaaaagtc ggggtacggt cagtcacatg acacctcctt acgaaatatc ggtccttttc
      661 taaggtatcg gtacgccggt gttttactct agcgctgcgt ctgcctcctc tctgaggtct
      721 ctatcactat tatctaacaa accgggcatc gggtcaatat gcatgacgtg tttttgtagg
      781 tcaatttgtt taatcacgtc ttttatcgcc ggaatcagta cctgcgacag gtcgggcttt
      841 ttcacaacgt agacatcatt gctgccggtc gaaataatat caacaatggt gccgagatag
      901 gtttcatcga gtgtgaagac gcgcaggcca atgatatcat gctgataata cgacccggct
      961 ggcaattccg ccaggcgcga gaggggaatg gtcaagtcgg cattgcgcaa agattcggca
     1021 gcgttggcat cacgcagacc gcgcagcttg agcaagatca tttcgccctt gtagggacgc
     1081 acactctcga tagggtacga acggcgctcc tcgccaacgt agatcacatc caattgctcg
     1141 aagcgattgg gaacatcggt caacaggcgg actttcaatt cgccacgcac accaaaaagg
     1201 gccactactt tcccaatagt agcccattct gtctgttgtt gctcggacac tgtcacaaat
     1261 accctcttac agtaagggcg tacccttgtg gtcgccctgg aaggatttta atacgtcgtc
     1321 ttcctggaag gaattcaata tactgccgac ctggcagtat ttcaatacgc tattgaattt
     1381 cgagtacggt atggcggcct tcgcgtgtgc cgccgacacg cagcagcgta cgcatggcct
     1441 tcgcaactcg cccctgacgg ccaatgactt taccgaaatc gtcgggcgcg acgtgcaact
     1501 ccagcaccag ggcattgcgg tcgttacgca cttcgcgtac ctgcactgcg tcaggcgcat
     1561 caaccagcga ctttgcaacg tactctatga gtttgcgcat gtgcttacct tctctgtaat
     1621 ctgtaatacg cgctgtttat gcttccgccg gtggctttgc agcagccttc atttgctcaa
     1681 agcgatcggt gatgccggcg cgtttgagca gggacgcgac ggagtcggtc ggctgcgcgc
     1741 cattcttgag ccagccgaga gccttctcct cgtcgatatg gatggcggac ggctcgacca
     1801 ggggattata ccagccgata ttttcgatga aacgcccatc gcgcggcgag cgcgagtcgg
     1861 caacgacaac acgatagctg ggggctttct tcttccccac tcgtttgaga cgaatcttga
     1921 cagtcatgcg tttttctctc acctcttccc cctgggcctg actgactcgc ttctctcaac
     1981 gtctgtcccg gtggggggat attcttgatt gtttgtggca acgagtgccg cgccgatagg
     2041 cggcgcagcg gttatgcttc ttgcactaag gcacacttag gatagcagaa gaggcgaaaa
     2101 ttgtcaatca aaattgcaat cggctccggc gtatgataag gtatcatgta cctcatctac
     2161 acgagaagca ggaaaggcga caaacatgac gactgaacag ggcggaatag tagcgctggt
     2221 tggatcgggc gaatttctcg aaacgacgct gcccgtcgat agatacctgg ttgagcagat
     2281 gcagcagagg caattgacgc cgcgcgtggc catcttgccg acggcctccg cgcccgacgg
     2341 cggggatgtg ccgcagaact gggcgaagat gggtgtcgag cattttgccc ggcttggcct
     2401 ggctgccgag ccagtgatgc tgctgacgcg ccaggatgcc gataatcccg ccattgtcgc
     2461 gcagctggcc gccacgaact tcgtctactt ctccggcggc aaaccgcgct acctgatgga
     2521 gacattgcag aagagcgccg cctggcaaac catcctggac atgttccggc agggcggagt
     2581 cgtggcgggc tgctcggcag gcgcaatggt cctcggcggg gcattcttcg acttccccca
     2641 gatctggagt acaaagcccg cgctcggtct cgttcccggc atcgtcgtca tcccacactt
     2701 cgacgagatg ccgaagttga taacctccac gattggcggc ggcaaacgtg aaggagtcgt
     2761 tgtcggcatc gaaggtggca ccgcgctggt cgtctcgcac gaacactgga tcgtgcaggg
     2821 caaaggcggc gtgacggtct tcaacggcaa acgcagacag cgctatcaag cgggtgagac
     2881 gattctcatg aagcgcatat gatagaatcg atacaaatat acccaacttt cttgggagat
     2941 tacttatgaa taaagagcat ccacgcacac cgggccgcta tggcaccgaa gagatcgagc
     3001 agagcaggct ggaggcatgg ccaaattcca acccggagcg cgactatatc acccatctcg
     3061 aaataccgga gttcacctgc ctctgcccgc gctccggctt tcccgacttc gccaccatcg
     3121 tcatcgacta cgttcccgat cagtctctcg tcgaactcaa gagcctgaag ctctacatca
     3181 accagtatcg caaccggcag atcagccacg aagcagcaac caacaccatc cttgacgaac
     3241 tcgtcgccct gcttgcgccc cgctggatgc gcgtcgtggc agatttcacc gtacgcggca
     3301 acatcaagac tatcatcttc gccgaacaca gcgcgcccgg ctacacaggg ccgcgaccgg
     3361 agtatcgaag gtacatgcag ggctaaaggc aaggagcaaa ggctatgttc tgtatctatt
     3421 gcggcgaaca ttatcccaaa gaggctacat tctgtccaaa atgtggcagg gcggtgcata
     3481 ccagcctact cctcgatagc gcctccagcg ggtctgttcc agtgaccgcg ggggaggctg
     3541 caataatcgg ctcaccatcg tggcgactcg atatgcacac gagcgcgaaa tggatacgca
     3601 gagttggcat tcccctggcg gcgctggcct ggcttgtgct ggtagcggct ctactctggg
     3661 cagcatcgca cgtggcgcgt tcgctgatcc tgctcgccat tgccgcgatc ctggcgtttg
     3721 cgctcgcgcc catcgtgaaa ttgttgcagc ggttcatgcc tcgcctgctg gcaatcctgc
     3781 tcgtctacat cgtggtcatg ggcggcatca gcatcgttct gtatttcctc atacgcgccg
     3841 ccattgtgca ggccgaagaa ctcaaaaacc aggtgagcag cctgctgcaa ccggggcaat
     3901 catcatcgct gcaagcgctg ctggcatcgc tgggcataga cgcgacccag ctacagaatg
     3961 tgcgccagac catcactgca cacctgggcg ggctggtcgg cgatattgtg ccagtgctga
     4021 gcggtctatt cgacgctgtg ctggatgtga tcattgttac catgctcagc atctacctgc
     4081 tgctcgacgg ctcgcgcgtt ttccgttggc tgcgcgaaaa catgccggtc ccgcagcaga
     4141 aacgcggcta cttcctgctc gacacgctgg aatctgtcgt tggcggctat attcgcgggc
     4201 aactgacgct ctcgctactg atcggtgtac tggtcggcgt gggtatgacg atcttccgtg
     4261 tgccgcatcc cgtattgctg ggagaactcg cctttctgct ggcgttcatt cccgtgctgg
     4321 gaacattcat ctccggcgcg ttctgcatcc tgctgtcatt gaccgccacg aatagctggg
     4381 tcgtcgccct gacgggacag acgtggatac tcgccattat cgtgctgctc tacttcgtcg
     4441 ccatgcattt tatcgaaagc cacatcgtcg gcccgcgcat cgtcggccac gccgtcgggc
     4501 tgcaccccgt cgtctctatc gccgccctgg tagcgagcgc cgaactcttc ggcatcctcg
     4561 gtgcgctctt cgtggttccc ctcgtcggca tgctccaggc catcatcgtc tggctctggc
     4621 gagactggcg cgccgcgcac cccgaccagt tccccggcaa gccggtgacg gtggagagcc
     4681 gcgtaagcgg ggatgaagcg caggcggcgg ttgagtagtt gagcagcgag atatggtatg
     4741 atgtcccata gaatacagga caatatcagg agggaacagt ggctcaacgc gaatactcag
     4801 tcatgagcgt cgaagactac ctggtactca gcaggagcag caaggatgcg cgctacgaat
     4861 acctcgatgg cgacctgcgt atgctggccg gaggcagtcc agaccactct atcatcatcg
     4921 ccaacctgac ggctacgatt aaagcgccac tcaagggcgg ttcatgccgc gtctacaatt
     4981 ctgatgtaca attgcggctg tctgaaaagc gctacgtctt ccccgatatc gcggtcagtt
     5041 gcgacgagcg cgacaggaac cagaaagaaa tgatacgcta cccccgtgtc gtcgtcgagg
     5101 tactttctcc cagtaccgag gccattgacc gagggaaaaa agcggcctac tatcgagcat
     5161 gtccaaccat acaagaatat gtcatggttg actctatcga ggtatttatt gaagttcacc
     5221 ggcgtacaga agagctatgg acgatcaata cctttgaagc aggagacaac gtgacgctgg
     5281 aaagcctcga catccaattc ccaagagagg acgcctacga gggaaccagc cttcttaacg
     5341 agccatagtc tctcctttat ggtacaatac agccaagaga gatcattaag agtatgccgt
     5401 acaaaaacga accgcttaga aatacctact acctgcaagg ccaatatact tttgaacaag
     5461 aaccaactgg catcactgct cacct
//
LOCUS       DASZAK010000019         1980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_35844, whole genome shotgun sequence.
ACCESSION   DASZAK010000019 DASZAK010000000
VERSION     DASZAK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35844"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..940
                     /gene="radC"
                     /locus_tag="VGT44_00310"
     CDS             167..940
                     /gene="radC"
                     /locus_tag="VGT44_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV2579264.1"
                     /translation="MPTAAKLEQVEQTEMSNKSNSNITPFPARLEYHPTIHDLPSEER
                     PRERLRNHGARALSHAELLAIILRTGTAQDNVIELAGKLLTKYGGLAGLMRAEFMELC
                     AEHGLGEAKTAQIKAALEIGRRLSVAQPEEKYQIKSPADAAALVMMEMTFLDHEQMRI
                     LVLDTKNQVVENIPRYQGTVNSSVLRAAEVYRPAVLRNCPGVIICHNHPSGDPTPSPE
                     DITVTQQLVQAGQQLDIELVDHLIIGNHRFVSLKERLNW"
     gene            1095..>1980
                     /locus_tag="VGT44_00315"
     CDS             1095..>1980
                     /locus_tag="VGT44_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV2579265.1"
                     /translation="MKILHVSDIHYRESADSWTLLKEATRQFERLRPDYILITGDLTY
                     DGLDEQFERVKRFISSLDFCETLIIPGNRDAVRSFVPVAIERSDLEYPQFMSSEPARM
                     LDEMETLENVHHKGRGKFFEHFDASEYFYRANSIAAVGLNSAPEITARQMDMVVQHFS
                     KGQPGELRIFCAHHSFLPVPTKKLKPGDTVPRAADILQALIELNVDLLCCGHIHRSHV
                     WDLSDGRHRLLCCNAGSLLDTSGKKDNGYLEIDIGHDLNITKHSLFSRYTERLYHRAR
                     FGDILKARQESQPIDTFDY"
BASE COUNT          470 a          563 c          525 g          422 t
ORIGIN      
        1 gctttcttgc atctacctct atgacgcctg agaatcgtca aaccctgtga tggttttacc
       61 gcttgcttcc tattttgcct attgcatccg ggcaattgtc ccgttattgt gtagaactat
      121 aaagatcatc ccaacgaaag ggtgacaaga gaggagccac gtccatatgc caaccgccgc
      181 taagctagaa caggtcgagc agaccgaaat gagcaataaa agcaacagta atattacccc
      241 attcccagct cgcttagagt accatccgac catccacgat cttccaagcg aagagcgacc
      301 gcgcgagcga ctgcgcaatc acggcgctcg cgcgctttcc cacgcggaat tgctggccat
      361 catcctgcgc accggcacgg cgcaggacaa cgtgatcgaa ttagcgggca agctgctgac
      421 aaaatacggt gggctggcag gcttgatgcg cgctgaattc atggaactgt gcgcggaaca
      481 cgggctgggc gaggccaaga ctgcgcaaat caaggccgct ctggagattg gtcggcggct
      541 cagcgtggca cagccggaag aaaaatacca gatcaaatcg ccggcagacg cggcggccct
      601 ggtgatgatg gagatgacct tcctcgacca cgagcaaatg cgtatcctcg ttctcgacac
      661 gaaaaaccag gtagtcgaga atatcccgcg ctaccagggg acagtcaaca gctctgtgct
      721 gcgcgcggca gaagtctacc gccctgccgt cctgcgcaac tgcccgggtg tcatcatttg
      781 tcataaccac cccagcggcg acccgacgcc ctcgccggaa gacatcaccg tcacccaaca
      841 actggtgcag gctggccagc agctcgacat cgaactggtc gatcacctca tcatcggcaa
      901 ccaccgcttc gtcagcttga aggaacgact gaactggtaa agcctggata tgtggcagtg
      961 ttccctcgcg gtcgcccttt tcagacattg tttcttattg cattttctct tcccatgcta
     1021 tatcctctag ggtaggaggg caggtttcat gccggtagag atttacggcg aacagatgga
     1081 ggagggaaga tcaagtgaaa atattgcatg tttctgatat ccattaccgc gagtcggctg
     1141 atagctggac gttgctgaaa gaggcgacgc gccaatttga gaggctgcgg cccgattata
     1201 ttcttattac aggcgacctg acatatgatg gcctggacga gcaattcgag cgtgtcaaac
     1261 gcttcatctc ttccctggat ttctgcgaaa cgctgattat ccccggcaat cgcgacgctg
     1321 tgcgcagctt tgtgccggta gcgattgagc gcagcgacct tgaatacccc caattcatgt
     1381 cctctgaacc cgccagaatg ctcgacgaaa tggagacgct ggaaaacgtc catcacaagg
     1441 gacggggtaa atttttcgaa catttcgatg cgtcggagta tttctatcgc gccaacagca
     1501 tcgccgccgt tggcttgaac agcgcgccag agattacagc gcgccagatg gatatggttg
     1561 tgcagcattt cagcaagggc cagccgggag agttgcgcat tttctgcgcg catcacagct
     1621 tcctgccggt tccaaccaaa aagctcaagc cgggcgatac cgtgccgcgc gccgcggaca
     1681 tcctgcaagc gctgatcgaa cttaacgtcg atctgctctg ctgcgggcat attcaccgca
     1741 gccatgtttg ggatctgtcg gatgggcgac atcgcttgct ctgctgtaac gccggttcct
     1801 tgctcgatac atcgggcaag aaagataacg gttacctgga aatcgatatc ggtcacgacc
     1861 tgaatatcac taaacatagc ctgttttcgc gttacaccga gcggctctac catcgcgcgc
     1921 gcttcggaga catactcaaa gcgcgccagg agagccagcc tatcgataca tttgactaca
//
LOCUS       DASZAK010000020         3097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_35845, whole genome shotgun sequence.
ACCESSION   DASZAK010000020 DASZAK010000000
VERSION     DASZAK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35845"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VGT44_00320"
     CDS             <1..508
                     /locus_tag="VGT44_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579266.1"
                     /translation="CGSFEQSGAQLLSASCTPTRPGGCRGPATGGPSAIRTSPATGGA
                     ARTIYSSQSQAFMTLHAVDAQTLLLYIENTQGDLSQNGLWKLKSDGTGLTRLTTVGGQ
                     LCSDLGYPATWPQITSNSQFYALRQIDPVSFYSALQVGSLNGGAPTTFATKSASVGIM
                     SLVGMVSL"
     gene            complement(749..1435)
                     /locus_tag="VGT44_00325"
     CDS             complement(749..1435)
                     /locus_tag="VGT44_00325"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV2579267.1"
                     /translation="MTQHHEPVAIDSLAAEIDARLQALPNPTTEPVRAVRREYSRRIA
                     GASPQEVIALALRLLDRHRFVAYELVCHHRTALRSLGERELELFGRDMASWEAVDTFA
                     CYLAGPAWRERQVPDALIEGWAHSADRWWRRAALVCTVPLNLTARGGRGDTARTLLIC
                     RLLIADRDDMVVKAMSWALRQLCTRDPQAVRDFLAEYNGRLAARVVREVQNKLTTGLK
                     NPRNKPITKQ"
     gene            1662..2864
                     /locus_tag="VGT44_00330"
     CDS             1662..2864
                     /locus_tag="VGT44_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2579268.1"
                     /translation="MQRTNNGLELGVAEPDAHSSSEGPSKTFPTSPLPLSPQVASSLL
                     QEGISVQLWLDISPDALVVVNAAGQIILVNRQGETLFGYTRQELVGQPLEVLLPERLR
                     AAHVRHREHYATSPHIRPMGVGLELSARRKDGTEVPIDISLSPLRFDEAFYVMAAIRD
                     ITERKLLEQRAQLEQRKDDFISMASHELKTPLTSLTAYTDLLQRLLDRENNSQAGHYL
                     ARMNVQLTKLTKLIADLLDISKVQAGKLVFAEEAIRIDDLVREEVGSFQPTTPRHRIS
                     IEGETDCEVSGDRDRLGQVVINLLSNAVKYSPQAEQVIVRLESSASECIVSVQDFGIG
                     IPVGYTERIFERFYRISTNQKQHFSGLGIGLYISSEIIQHHSGRIWVESVEGVGSTFS
                     FALPLKRA"
BASE COUNT          639 a          903 c          916 g          639 t
ORIGIN      
        1 ctgcggctcc tttgaacaga gcggcgcgca gctgctcagc gcctcgtgta caccgacgcg
       61 acccggcggc tgtcgcggcc cggcgaccgg cggccccagc gcgatccgaa ccagcccggc
      121 gaccggcggc gctgccagaa cgatctatag cagtcagagc caggcgttca tgaccctgca
      181 cgccgtcgat gcgcagacgc tcctgctgta tatcgagaac acgcagggcg atctgagtca
      241 aaatggcctc tggaaactca agagcgacgg caccggcctc acgcgcctca cgacggtggg
      301 aggacagtta tgttccgacc tgggctaccc ggcaacctgg ccgcaaatca ccagcaacag
      361 ccaattctac gcgctgcgcc agatcgatcc cgtctccttc tacagtgcgc tgcaagtggg
      421 cagcctcaac gggggcgcgc caaccacgtt tgcaacaaag agtgcgagcg ttggcattat
      481 gagcctggtg gggatggtat ctctgtaggc gccggttcac cgtgcgcacc gccgattgat
      541 cggcctggaa tgcccgtcga tcaagtacat gtcgcgataa tgcggataaa caaaatatcc
      601 cggctgccag agcgggaaga cgagggcgat cgctaatgca gatagaccaa atacccttgc
      661 ggtcgccctc gttgcccaga tcaggagaag accgcctgtt gaggacacgc aaatcagggg
      721 tggagaccct tgcggtcgcc ctggggtctt actgtttcgt gatgggttta ttccggggat
      781 tcttcaaccc tgtcgtgagc ttgttttgca cctcgcggac tacgcgggcg gcgaggcggc
      841 cattgtattc ggccaggaag tcgcgcacgg cctgcgggtc gcgcgtgcat agctggcgca
      901 gcgcccacga catggccttc accaccatat cgtcgcgatc ggcgattagc aggcgacaga
      961 tcagcagggt gcgcgccgta tccccgcggc cgccgcgcgc ggtcagattg agcggcacgg
     1021 tgcagacgag cgcagcgcgc cgccaccagc gatccgccga gtgcgcccag ccttcgatca
     1081 gcgcgtccgg cacctgccgc tcgcgccagg ccggtcccgc caggtagcag gcgaaggtgt
     1141 ctaccgcttc ccagcttgcc atatcgcgcc cgaacagctc tagctcgcgt tcgcccaggc
     1201 tgcgcagcgc ggtgcgatga tggcacacca gttcataggc cacgaagcgg tgccggtcga
     1261 gcaggcgcag agccagcgcg atgacctcct ggggcgacgc cccggcaatg cgccgggagt
     1321 attcgcgccg caccgcccgc accggctccg tcgtcgggtt tggcagggct tgcaggcgcg
     1381 catcgatttc cgccgcgagt gagtcgattg ccactggttc atgatgttgc gtcattgcgg
     1441 tttacccctt atagcatagt tgttctgtat tatatcctct tgtgcagcgt ttatcatcta
     1501 ttattcctaa tacgttgtat aaatcttctg ttctatggca taattgggtg gtgtaagcat
     1561 ctcttgcgtg gaaagtgaac atgtgcatct gctgcccggc ggcagagcag catccagata
     1621 tccttgggca gggagatagg tgtgcttaga aggaggtatg tgtgcagagg actaacaatg
     1681 ggttggagct gggagtagca gaaccagatg ctcattcttc cagtgagggg ccgagcaaga
     1741 ccttccccac ttctccgctt cctctttccc cgcaggtcgc ctcttcgctc ctgcaagagg
     1801 ggatctcggt acagttgtgg ctggatatct cgccggatgc gctggtcgtc gtcaatgctg
     1861 ctggtcaaat tatcctcgtc aaccgccagg gcgaaacgtt gtttggctac acgcgccagg
     1921 aattggtcgg ccagccgctc gaagtcctcc tgcccgagcg attgcgcgcc gcgcatgtgc
     1981 gccaccgcga gcattatgct acctcaccac acattcgccc gatgggcgtg ggtctagaac
     2041 tgtcggcccg gcgcaaagat ggcaccgaag tgccaatcga tatcagtttg agcccactgc
     2101 gtttcgatga ggcattctat gtgatggctg ctattcgcga tatcaccgag cgcaagctcc
     2161 tggaacagcg cgcgcagctg gagcagcgca aggacgactt catcagcatg gccagccatg
     2221 aactcaagac gccgctcacc agcctgaccg cttataccga tctcctgcag cgactcctgg
     2281 accgggagaa caacagccag gcgggccatt acctggcgag gatgaacgtc cagttgacga
     2341 aactcaccaa gctcattgcc gacctgctcg atatttcgaa agtacaggcg ggcaaactcg
     2401 tgtttgcaga ggaagctatc aggattgacg atctggtgcg cgaggaggtc ggatctttcc
     2461 aaccgacaac tccacggcac cgtatcagca tcgagggaga gacagattgt gaggtgtctg
     2521 gcgaccggga ccgacttgga caggtggtca ttaatctctt gagcaatgcc gtcaagtact
     2581 cgccgcaagc ggagcaggtg atcgtgcgcc tggaaagttc cgcttccgaa tgcattgtca
     2641 gcgtccagga ttttggcatt ggtatccctg tgggctacac ggagcgcatc ttcgagcggt
     2701 tttatcgtat ctcgaccaac cagaagcaac atttctctgg tctgggcatt ggtttatata
     2761 tttcctctga gattattcag catcatagcg ggagaatctg ggtggaaagt gtcgaggggg
     2821 tgggatctac cttttccttt gcgctacccc tcaagcgagc ataaaagttg cggatatgtc
     2881 acagcaagca atggcagctt gtggagcaga tggttttctc gccaagcctt ttcggatagc
     2941 ggaattgctc gatctcgtgg caagaaagat cacgccgaga cagcagaacc tgaactgacg
     3001 tgggatggtt tcaccatccc acgctgcgct caggatgaca gccagtaact tcaatcatct
     3061 ggcgaactta ctaaaaactt gacttaatta agaaaat
//
LOCUS       DASZAK010000021         2802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_639384, whole genome shotgun sequence.
ACCESSION   DASZAK010000021 DASZAK010000000
VERSION     DASZAK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639384"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VGT44_00335"
     CDS             complement(<1..532)
                     /locus_tag="VGT44_00335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2579269.1"
                     /translation="MVVGDVTTAVDVLVVGAGPGGYVAAIRAAQLGHHVTLIDSGPLG
                     GACLHRGCIPLKALLSASERYAQTRQEDLAAMGIHAAPVTFNWSEMQAWKQSVVDRLA
                     DGVRRLVAGNRIDYVHGTGWFMNAQEMRVEGEHGSLRFKFDHCIIATGASAASIDNLP
                     YDGSKILTSEQALMLSE"
     gene            complement(543..1823)
                     /locus_tag="VGT44_00340"
     CDS             complement(543..1823)
                     /locus_tag="VGT44_00340"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEV2579270.1"
                     /translation="MSELKLADLGEGMHEAEVVEWLVKEGDTVKIDQTVVKVETDKAI
                     VEIPSPVTGRVTEIRVPNGEIAKVGDVLVVFDAPNAEKNENGTSARHDGVTPSAQPPA
                     MATPALSQPQPSMPKHRVLAAPAVRKLAFELGVDLEQVTPSLSGGRVSLDDVKAYAAR
                     PPSSSPATVSDSATASVGAQFIAPTFSPVISQSEDERQPLTGLRKRIAERMERSWRTI
                     PHATAFGELDGGALIALRESLKPAAEKRGVHLTYMPLLIKLLIPVLKEFPIFNASLDE
                     EKREIIYRRSYHIGVAAATPEGLLVPVLRNADRLTLVEIANNVERLIEGAKKRTLALA
                     EVSGSTFTLNNVGSFGGSTGTPIINYPEVAILAVGRLQEQAVVRQGTIIARPIMPLAL
                     SFDHRLIDGALAGAFLARFKELVENPQQLMLDMV"
     gene            complement(1856..>2802)
                     /locus_tag="VGT44_00345"
     CDS             complement(1856..>2802)
                     /locus_tag="VGT44_00345"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV2579271.1"
                     /translation="ALALELKRDSRVVVLGLDIGANGGVFRVTEHLQEQFGEKRVFDT
                     PLSESAIIGSSVGMAVYGLRPIAEIQFAGFLFVCMNQLVSQAARIRFRSAGAYTCPMV
                     VRAPYGGGVRTPELHSDSLEGVFLQTPGLKIILPSNPYDAKGLLASAVLDPDPVLFLE
                     NIKLYRSFRQDTPQDYYTVPLGQARVAQEGSDVSIFAYGAMVNVALEAAKLAQQEMGS
                     SVEVIDLRTIWPLDEDAIAASVEKTGRAVIVHEAPRAGGIGAEITAIINERCFYSLLK
                     PIGRVTGYDTPFPVPGQEDYYVPSPAKVLTALREIMEP"
BASE COUNT          561 a          858 c          822 g          561 t
ORIGIN      
        1 gttccgacaa catcaacgcc tgttcggatg tgagaatctt gcttccgtcg tagggcagat
       61 tgtcgattga ggcggcgctc gcgccggtgg cgataatgca atgatcgaat ttgaaacgga
      121 gtgagccgtg ttcgccctcg acgcgcatct cctgcgcgtt cataaaccag ccggtaccat
      181 gcacataatc gatacgattg cccgcgacga ggcggcgcac gccatcggcc agccgatcaa
      241 ccacgctctg tttccacgcc tgcatttctg accagttgaa tgtaaccggc gcggcgtgaa
      301 tgcccatcgc cgccaggtct tcctggcgag tctgcgcata gcgttcgctg gcggaaagta
      361 gggccttgag cgggatacag ccgcgatgca ggcaggcgcc gcccagcggt cccgaatcga
      421 tcaatgtgac gtgatgaccc agctgcgcgg cgcgaatagc cgcgacatag ccgccagggc
      481 cagcgccgac gaccagcaca tcaactgcgg ttgtgacatc tccaacgacc attggctctc
      541 ccctatacca tatccagcat gagttgctgc gggttctcca ccagttcttt gaaccttgcc
      601 aggaacgcgc ccgccagcgc gccatcgatc aggcggtgat cgaaggaaag cgccaggggc
      661 atgattggcc gcgcgatgat tgtgccttga cgcacaaccg cctgctcttg caacctgcca
      721 acggcgagta tggcgacctc aggatagttg atgatgggtg tgccagtact gccgccgaag
      781 ctgccaacgt tgttgagagt gaaggtgctg ccgctgacct ccgccagcgc cagggtgcgc
      841 ttcttcgcgc cctcgatcag ccgctcaaca ttgttcgcaa tctccacaag cgtgagacgg
      901 tccgcgttgc gcagcaccgg cacaagcagg ccctccggcg tcgctgcggc aaccccgatg
      961 tgatacgagc gcctgtagat aatctcgcgc ttctcttcat ccaggctggc attgaagata
     1021 ggaaattctt ttaagacagg gatcagcaac ttgatgagca atggcatgta agtcagatgc
     1081 acaccgcgct tctctgccgc cggtttcagg ctctcgcgca gagcgatcaa cgcgccacca
     1141 tcgagttcgc caaacgccgt tgcgtgtgga atggtacgcc aggaacgctc catacgttcc
     1201 gcgatacgct tgcgcaggcc ggtgaggggc tggcgttcgt cctcggattg tgagatgacg
     1261 ggagagaaag tgggcgcaat gaattgcgcc cctacagatg ccgttgcaga atcagataca
     1321 gtcgcaggag agctggatgg agggcgagca gcataggctt tcacatcgtc aagggaaaca
     1381 cgtccgcccg aaagtgatgg cgtgacctgt tccaggtcta cccccagttc aaaggcgagt
     1441 ttgcgcacgg caggcgcggc cagcacgcga tgtttcggca tcgaaggctg aggttgggat
     1501 agagcaggcg ttgccattgc tggcggctga gccgaaggtg tcaccccatc atgcctggcc
     1561 gatgtgccat tctcgttttt ttctgcgttc ggcgcgtcga agacgaccag cacatcgccc
     1621 accttcgcga tctccccgtt cggaacgcgg atttctgtca cccgccctgt caccggcgac
     1681 gggatctcga caatggcctt gtcggtttca accttcacta ccgtctgatc gattttgact
     1741 gtatcgcctt cttttaccag ccattcgacc acttcggctt cgtgcatccc ctcgcccaga
     1801 tcggccagtt tcagttcgct catgttatgc tctccataca actttttcgg ccaggttagg
     1861 gttccatgat ctcgcgcagc gccgtcagca cttttgcggg agacggcaca taataatcct
     1921 cctgtcccgg cacggggaaa ggggtatcat agcccgtcac gcgcccgatt ggcttgagca
     1981 aggagtagaa gcagcgctcg ttgatgatgg cggtgatctc cgcgccgatg ccgcccgcac
     2041 gcggggcttc atgcacgatg acagcgcggc ccgtcttctc gaccgaggcg gcgatggcat
     2101 cctcgtcgag cggccagatc gtgcgcaggt cgatgacctc aacgctgctt cccatctcct
     2161 gctgagccaa cttggccgct tcaagcgcga cgttgaccat cgcgccatag gcgaagatgc
     2221 tgacatcact gccttcctgc gcgacccgcg cctgacccaa tggcacggtg taatagtcct
     2281 gcggcgtgtc ctggcggaac gagcggtaga gcttgatgtt ttccaggaaa aggaccgggt
     2341 cggggtcaag caccgccgag gccagcaggc ccttcgcatc gtagggattg gaaggcaata
     2401 tgattttgag gccgggggtc tgcaagaaga cgccttccag actgtccgag tgtagttcgg
     2461 gtgtacgcac gccaccccca tagggcgcgc gcaccaccat tgggcaggtg tacgctcctg
     2521 cgctgcggaa tcgaatgcgc gccgcctgcg atacaagctg gttcatgcac acaaagagaa
     2581 aaccggcaaa ctgtatctcg gcgataggtc gcagcccata gaccgccatg ccgacagaac
     2641 tgccgatgat ggccgattcg gatagggggg tatcgaagac gcgcttttcg ccgaactgtt
     2701 cctgcagatg ttcggtcacg cgaaacacgc ccccattcgc gccgatatcc agcccaagca
     2761 cgacaacacg gctatcgcgc ttcaactcaa gcgcgagcgc cg
//
LOCUS       DASZAK010000022         5232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_213444, whole genome shotgun sequence.
ACCESSION   DASZAK010000022 DASZAK010000000
VERSION     DASZAK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5232
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213444"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1806
                     /gene="fdrA"
                     /locus_tag="VGT44_00350"
     CDS             229..1806
                     /gene="fdrA"
                     /locus_tag="VGT44_00350"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEV2579272.1"
                     /translation="MHIAYLLKRHTYHDSITLMVVAQAVKEQARVDEVGVVMASEANR
                     LLLQESGIWPAAVDALADQPGPDDLLIVVRANDEAYAQAVLALVEQQLASRGRQEAPG
                     GAVSTSHARSLEMALRNDRQANLALISVAGEHAWLEAEQALRAGLHVFLFSDNVSIAQ
                     ERRLKTLANSKGLLLMGPDCGTAIINGVGLGFSNVVPRDSIGIVGATGTGMQQLSCLI
                     ASAGLGVSQAIGVGGRDLSEAVGGIMMRRGLALLTDDPQTEVIVLVAKPSSERVVVEV
                     LQAAQIDKPVVAVFLGAPPEQFREAAGKVALAQTLTEGAELAVALAGGDRRKVPGEAA
                     LLEHQARLAVECAALSPSQRYIRALYSGGTLCYEAMYLLSEHLGAVASNIPLRAEWAL
                     AAGETFRGHTALDMGEDQFTKGKPHPMIDPSLLLRYVTQAAHDQETAVILLDIVLGYC
                     AHPNPAGIYAPVIARCRDEASAAGRRLPVIVSLCGTEQDLQQRSAQRSMLEAAGALVF
                     ESNVAAARAALTCLSER"
     gene            1814..3238
                     /locus_tag="VGT44_00355"
     CDS             1814..3238
                     /locus_tag="VGT44_00355"
                     /inference="COORDINATES: protein motif:HMM:NF018275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HEV2579273.1"
                     /translation="MTETSDLFTSPVRAVNVGSDLFADALAAQGAAVARVAWQPPAGN
                     SDDALGVLLPDVRVDKANQEATRRMIAARPRLVDVRPAREAIPGMHQHLLLHAGPPIG
                     WERMSGPLRGAIIGALIYENLASDSAEAERLAAGGEIEFAPCHHFNTVGPMAGVVSAS
                     MQVQVIEEPVYGHQTYSTLNEGFGKALRFGANSPEVIEKLRWMEQTLGPALARAIQKM
                     GGLDLKTVTAQALQMGDECHNRNKAATSLFIREVAPYLVETSKSVEEVARVLRFINGN
                     DHFFVNLSMATCKAMTLPARGIPFSSVVTTMARNGTDFGIRVSGLGDRWYVAPAQRIN
                     GLYFSGYSEEDANPDIGDSSITETAGIGGFSMAAAPAITQFIGGTPADALRYTTEMYD
                     ITVAENEQYRLPPLGFRGAPLGIDTRQVVRQNLLPVINTGIAHRLPGVGQVGAGIVRP
                     PMACFVAAARDLAASIKADVPPNQ"
     gene            complement(3344..4435)
                     /gene="gap"
                     /locus_tag="VGT44_00360"
     CDS             complement(3344..4435)
                     /gene="gap"
                     /locus_tag="VGT44_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV2579274.1"
                     /translation="MRIGINGFGRIGRLILRSALARRVNLDIVAINDRGDAEINAHLF
                     QFDSTYGPFRGQVAVSDGQIIIDGRPITIVSHLNPLDIPWADMGVELVIEATGVFTNA
                     DQAVAHHLAGAERVLVTAPSKGADVTLVMGVNEQDYDPEDHRIISAASCTTNALAPLA
                     KVLHESFGLRSGMMTTVHAYTNDQRILDRSHKDPRRARAGAENIIPTTTGATRALGEV
                     IPALAGKFHGVSYRVPTLTVSVVDLVAELEDAVSVAEINAAFALAACGPMESVLGYTD
                     LPLVSSDFRGDSRSSIVDGLMTAVLPSEERLIRVVSWYDNEWGYASRVADLASFVSNY
                     ESRRKRMECARELEMALVEASLPWWRFTA"
     gene            complement(4438..>5232)
                     /locus_tag="VGT44_00365"
     CDS             complement(4438..>5232)
                     /locus_tag="VGT44_00365"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005655.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV2579275.1"
                     /translation="NRQQRKELGITPLNPACNYLDIMAQQLQLLRAGEPILKPHYDHK
                     LGNFGPPQYIVPRPFLIVEGLLGMYWQEMRNAYSVRVFLDPPEELRWRWKIRRDSSLR
                     GYSEREVLHELAIREHDSAAYIRPQRDHADLVVSFYPGESSKLERDGDAHLNVRLTLH
                     GTLRHPDLSEVIDEDQSGCVRSTLGREGGRAVEYLEIDGSISDEPARALERCILDHLD
                     ACGAASPADLGMFSDSQVRHSHPLALTQLILVYHLLRARLRDEVEV"
BASE COUNT         1026 a         1603 c         1615 g          988 t
ORIGIN      
        1 tacaaacaac atcggtaata tctcgtagga gccgatttat cgtgctcaca gccgatttat
       61 cggcacgaat ccccggtcat tggtttggac tggccgataa atcggctgtg agcacgataa
      121 atcggctcct acgaagtatc cgatcctggt cgttcaagtt cattatcggc catcatgggt
      181 gtgaacaggg ccaatccagg ccgataaagc aatagacagg aggatgctgt gcatatagcg
      241 tacttactca aacgccacac ctatcacgac tcaattaccc tcatggttgt cgcgcaggcc
      301 gtgaaggagc aggccagggt agatgaggtc ggcgtggtta tggcgagcga ggcgaaccgt
      361 ctgctgctcc aggagagcgg catatggcca gccgccgttg atgccctggc agaccaacct
      421 gggccggatg atctgctgat tgtcgtgcgc gccaacgatg aagcctatgc gcaggcggta
      481 ttggccctgg ttgaacaaca acttgcctcg cgcggtcggc aggaagcgcc gggcggcgct
      541 gtatctacgt ctcacgcccg ctcgctcgaa atggcgctgc gcaacgaccg gcaggcaaac
      601 ctggcgctga tctcagtcgc gggcgaacat gcctggctcg aagcagagca ggcgctgcgc
      661 gcgggattgc acgtctttct cttcagcgat aatgtatcca tcgcgcagga acgccgcctc
      721 aaaacgctgg cgaacagcaa ggggttgctg ctgatgggac ccgattgcgg cacagccatc
      781 atcaacggcg tcggcctggg ctttagcaat gttgtcccgc gcgactcgat cggtattgtt
      841 ggggcgaccg gcacgggtat gcagcagctc agttgcctga tagcctcggc agggctcggc
      901 gtctcgcagg cgatcggtgt gggcgggcgc gacctatcag aggctgttgg aggtatcatg
      961 atgcgccggg ggcttgcgct gctgacagat gatcctcaaa ccgaagtaat tgtcctggtg
     1021 gcgaagccat catccgagcg agtcgtcgtc gaggttctgc aagcagcgca aatcgacaaa
     1081 ccggttgtgg ccgtctttct gggcgcacct cctgaacaat tccgcgaggc tgctgggaag
     1141 gttgcgctgg cgcaaacgct gaccgaagga gcagaactgg ccgtcgcgct ggcgggtgga
     1201 gaccggcgca aagtaccggg cgaggcggct ttgctggaac accaggcgcg tctagccgtt
     1261 gagtgcgccg cgttgtcccc gtcgcagcgc tacatccgcg cactgtactc agggggaacg
     1321 ctctgttacg aggcgatgta cctgctcagc gagcatcttg gcgcggtggc ttccaatatt
     1381 cccctgcgcg ccgagtgggc gctggcggca ggcgagacgt ttcgcggcca taccgccctg
     1441 gatatgggcg aggatcaatt cacgaagggc aagccgcatc ccatgatcga tccctcgctg
     1501 ctcttgcgtt atgtcacgca ggccgcgcac gaccaggaga ccgccgtaat cctgctcgat
     1561 attgtgttag ggtattgcgc ccaccccaat cccgccggga tctacgcgcc ggtcatcgcg
     1621 cgctgtcgcg acgaggcgag tgccgccggg cgaaggctgc cggtcatcgt ctcactgtgt
     1681 gggacggagc aagacttaca gcagcgttcc gcgcagcgct cgatgctcga agccgccggt
     1741 gcgctggtgt ttgagagcaa tgtagctgcc gcgcgcgcgg cactgacatg cctgagtgaa
     1801 aggtgacatt accatgaccg agacatccga tctctttacc tctccggtcc gggccgtgaa
     1861 tgtcggttcc gacctgtttg cagacgcact tgcggctcaa ggtgctgcgg tcgcgcgagt
     1921 ggcatggcag cccccggcgg gcaacagcga tgacgcgctc ggtgtgttgc tgcccgacgt
     1981 gcgcgtagac aaagcaaacc aggaggcgac acggcgcatg atcgcagctc gcccgcgcct
     2041 ggtagatgtg cgaccggcgc gcgaggctat tccaggaatg catcaacacc tgctgctgca
     2101 cgccggtccg cccatcggct gggagcgtat gtccggcccc ctgcgcggcg caatcatcgg
     2161 ggcattaatt tacgagaacc tcgcatcgga tagcgctgag gccgaacggc tggcggcagg
     2221 cggtgaaatt gaattcgcgc cctgccacca cttcaacacg gttgggccga tggcaggtgt
     2281 cgtctcggct tcgatgcagg tgcaggtcat cgaggagcct gtctatggcc accagaccta
     2341 cagtacgctc aacgaggggt ttggcaaggc gctgcgtttt ggcgcgaaca gccccgaagt
     2401 catcgaaaag ctgcgctgga tggagcaaac actggggcca gccctggcgc gcgccatcca
     2461 gaagatgggg ggcctggatc tcaagaccgt gaccgcccag gcgctgcaga tgggcgacga
     2521 gtgccacaat cgcaacaagg ccgccacatc gctcttcatt cgcgaagttg ccccttacct
     2581 ggttgagacg agcaaatcgg tcgaagaagt ggcgcgtgtg ctgcgcttca tcaatggcaa
     2641 cgaccatttt ttcgtcaatc tctcgatggc gacgtgcaag gccatgacgc tcccggcgcg
     2701 cggcattccg ttcagcagcg tcgtcaccac tatggcgcgc aacggcaccg atttcggtat
     2761 ccgtgtgagc ggcctgggcg atcgctggta cgtggctccg gcgcagcgta tcaatggtct
     2821 ctatttcagc ggctattcag aagaggacgc taaccccgat atcggcgata gcagcatcac
     2881 ggagacggcg ggcatcggcg gcttttcgat ggcggcggcc ccggccatca cgcagtttat
     2941 cggcggcaca cccgccgacg ccctgcgcta caccaccgag atgtacgata tcacggtggc
     3001 cgaaaacgag cagtaccgac tgccgccgct gggttttcgc ggcgcgcccc tgggcatcga
     3061 tacccgccag gtcgtgcgcc agaacctgct tccggtcatc aacaccggca tcgcccaccg
     3121 tctgcccggc gttggccagg tcggcgcggg catcgttcgc ccgcccatgg cctgcttcgt
     3181 cgcggcggcg cgtgacctgg cggcgagcat caaagcggat gtgcctccta accaatgatg
     3241 tgtaggggcg caattcattg tgcccccatt ccctatcgat gcggcctggc aagcgggcgc
     3301 aattcattgc gcccctacac atcattggcc gattggcaaa ctatcaggcc gtaaaccgcc
     3361 accagggtaa cgatgcctca acaagcgcca tttcgagttc ccgcgcgcat tccatgcgtt
     3421 tccggcgact ctcatagttg ctgacaaagc tcgccaggtc cgcgacccgg ctggcatagc
     3481 cccactcgtt gtcgtaccag ctcaccacgc ggatcaggcg ctcctcggag ggcaagaccg
     3541 ccgtcatcaa accatccacg atggatgaac ggctgtcgcc gcgaaagtcg ctggaaacca
     3601 gcggcaggtc ggtatagccg agtacgcttt ccatcggtcc gcaggctgcc agggcgaagg
     3661 ccgcgttgat ctccgccaca ctcaccgcgt cttccagttc ggcaaccagg tcaaccaccg
     3721 aaaccgtgag ggtaggcacg cggtaggaga cgccgtgaaa cttgcccgcc agggccggaa
     3781 taacctcgcc gagcgccctg gtggcgccgg tcgtcgtcgg gatgatattt tcagccccag
     3841 cccgcgcgcg ccgtgggtcc ttatgactgc ggtcaaggat gcgctggtca ttcgtgtagg
     3901 cgtggacggt cgtcatcatg ccagatcgca gcccaaagct ttcgtgcaac actttggcga
     3961 gcggcgccag cgcgttcgtg gtacaggagg cggcagaaat gatgcggtgg tcttctggat
     4021 cgtagtcctg ttcgttcacg cccatcacca gtgtcacatc ggcgcccttg cttggcgctg
     4081 tcaccagcac acgctcggct ccggccaggt gatgcgcgac tgcctggtcg gcattcgtga
     4141 agacgccggt cgcctcaatc accagctcga ctcccatgtc ggcccagggg atatcgagcg
     4201 gattgagatg cgaaacgatc gtgataggtc gcccatcgat gatgatctgc ccatcgctca
     4261 ccgccacctg cccgcgaaaa gggccatagg tggagtcgaa ctgaaagagg tgggcattga
     4321 tctccgcatc ccctcgatca ttaattgcta cgatatccag gttaacgcgc cgggccagcg
     4381 ccgagcgcag gattaatcta ccaatacgcc caaaaccatt aatgccaatc ctcatgctca
     4441 tacctcaact tcgtcgcgca gcctggcgcg caacaggtgg tagaccagga tgagctgggt
     4501 cagcgcgagc gggtggctgt ggcgcacctg cgaatcggaa aacatgccca ggtctgccgg
     4561 tgaagccgcg ccgcaggcgt ccaggtgatc cagaatacag cgctcaagcg cacgggcagg
     4621 ctcatcagag atggaaccgt cgatctccag gtactcgacc gcccggccac cttcgcgccc
     4681 aagcgtagaa cgcacgcagc ccgactggtc ctcgtcgatc acctcgctca ggtcgggatg
     4741 gcgcagcgtc ccatgtaagg tcagacgaac gttgaggtgc gcatctccat cccgttcaag
     4801 tttggacgac tcaccggggt agaaggaaac cacgagatcg gcgtgatctc gctgaggccg
     4861 gatatacgcc gccgaatcat gttcgcgaat ggccagttcg tgcaacacct ctcgttcgga
     4921 atagccgcgc agcgacgagt cgcgcctgat cttccagcgc catctcagtt cctcgggcgg
     4981 atcaagaaag acgcgcacgc tgtaggcgtt gcgcatctcc tgccagtaca tgccgagcag
     5041 cccttcgacg atcagaaaag ggcgcggcac aatatactgt ggtggtccga aattgcccag
     5101 tttatggtcg tagtgaggtt tcagaatagg ctcgccggcg cgcagcaatt gcaattgctg
     5161 cgccataata tcaagataat tgcatgccgg attcaagggg gtgataccca actccttgcg
     5221 ctgttgccgg tt
//
LOCUS       DASZAK010000023         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_320006, whole genome shotgun sequence.
ACCESSION   DASZAK010000023 DASZAK010000000
VERSION     DASZAK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320006"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VGT44_00370"
     CDS             <1..401
                     /locus_tag="VGT44_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579276.1"
                     /translation="NYMTVSGVFRTVTASAQPAELGRYANQYPNQALQSGASYPRPAP
                     SPVPAYAPSPYQQSNGNGYGNGFDNGFGNGYNGAPQGRSQQMQPMQQPQYQPQQPHPF
                     YQPPQQPRSLRPPSPPAPTPARPPVPPEWQ"
     gene            complement(458..2041)
                     /gene="gpmI"
                     /locus_tag="VGT44_00375"
     CDS             complement(458..2041)
                     /gene="gpmI"
                     /locus_tag="VGT44_00375"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEV2579277.1"
                     /translation="MATRAGGRPRPFVLIIMDGWGINPRKEGNAIALARTPNIDRLAR
                     EWPHTAVRTSGEAVGLPEGQMGNSEVGHQNIGAGKRVLQDYTRVSESIKDGSFFQNPA
                     LLKAVEHVKKNGSQLHLCGLLGNGGVHAHETHLEALLQLAHMHEIERVYVHSFTDGRD
                     SSPTGGVEFMQRLEAHAREIGGEHAAKVATVSGRYYAMDRDNRWDRTSLAYFAMTKGE
                     GLQANSAIAAIQQSYDKQVTDEFIMPTVVMEDGHPVAVVKAGDALIHYNFRPDRARQL
                     TKAFVMQDLPPQAEGKFERGPRLDDLVYVMMTEYEEGLDAEVAYRADEVEMPLARVIS
                     EHGLRQFHTAETEKYAHVTYFINGRRETPFPGEDRLLVPSPKVPTYDLQPEMSAAGVT
                     DTAIERIRSGQYDLVIMNYANADMVGHTGVIDAAIKAVEAVDTGVGRVVEATLAVGGG
                     LLITADHGNAEQLIDYDTGKPFTAHTTNPVPLYLVAPQLAQARLRADGILADVAPTIL
                     NVMGIPQPKDMTGRTLIMI"
     gene            2208..2657
                     /locus_tag="VGT44_00380"
     CDS             2208..2657
                     /locus_tag="VGT44_00380"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV2579278.1"
                     /translation="MDKITAQNNGSVKDYIASLDEQTVKDSQVLIEMMQRISGHEPKL
                     WNVGTIGFDTYHYKYDSGREGDGHVLGFYPRKGKITVYLMDGTARYAALLAKLGKHTT
                     TGYCVYIKRLSDVELPILEQIVQQSYEYIKSKSQDGPLDRIVWQTEK"
BASE COUNT          624 a          889 c          778 g          628 t
ORIGIN      
        1 ataattatat gacggtgagc ggcgtcttcc gcaccgtcac cgcctccgcg cagcccgcgg
       61 agctgggccg ctatgccaat cagtacccga accaggcgct ccagtctggc gcgtcatacc
      121 cacgacccgc gcccagtcct gtaccggcct acgccccctc accctatcag cagagcaacg
      181 gcaatgggta cggtaacggt tttgataatg gcttcggcaa cggctataac ggcgcgcccc
      241 agggcaggtc gcagcagatg caaccgatgc agcagccaca atatcaaccc cagcagccgc
      301 atccgttcta tcaaccgcca caacagccgc gctcgctgag gccgccttcc ccaccggcgc
      361 cgactccagc aaggccaccc gtgccacccg aatggcagta aagaagccag ggtgatatag
      421 gccaaccagg gcgaccgcga gggtcgccct ggttggccta tatcataatt agcgttcttc
      481 ccgtcatatc ctttggctga gggatgccca taacgttcag gatcgttggc gcgacatcgg
      541 ccaggatgcc atcagcacgc agcctggcct gtgcaagttg tggcgcgacg aggtaaagcg
      601 gcacggggtt agtagtgtgc gcggtgaagg gcttgccggt atcatagtcg atcagttgct
      661 cggcgttgcc atgatccgct gttatcagca gaccgccgcc caccgccagc gttgcctcaa
      721 ccacgcgccc tacgcccgta tcgacggctt caaccgcctt tatcgctgcg tcgatcacgc
      781 cggtatgccc gaccatatca gcgttggcat agttcataat caccaggtcg tactggccgc
      841 tgcgaatgcg ttcaatggcg gtatcggtca cgcccgccgc gctcatctca ggctgcaaat
      901 cgtaggtcgg cacctttggc gacggcacaa gcaggcgatc ctctccgggg aaaggcgttt
      961 cgcgccgccc attgatgaaa taagtcacgt gggcgtattt ctccgtctca gccgtatgaa
     1021 actggcgcag gccatgctca gaaatgacgc gcgccagggg catctctact tcatcagcgc
     1081 gataggccac ctcggcgtcc agcccctcct catactcggt catcatcaca tagaccaggt
     1141 cgtccaatcg cggcccccgc tcaaatttgc cttcggcctg gggcggcagg tcctgcatga
     1201 cgaaggcttt ggtcaactgg cgcgcgcggt cgggccggaa attatagtga atgagcgcgt
     1261 ccccagcctt gacgacggcg acgggatgac catcctccat cacgacggtt ggcatgatga
     1321 attcgtcggt gacctgcttg tcgtaggact gctggatggc ggcaatggcc gagttggcct
     1381 gcaaaccctc gcccttcgtc atggcgaaat aggccaggct ggtgcgatcc cagcgattat
     1441 cgcgatccat agcgtagtag cgcccgctca ccgtcgccac cttcgccgcg tgttcgccgc
     1501 cgatttcgcg cgcgtgcgcc tccaaccgct gcatgaactc gacgccgcct gtcggcgaag
     1561 aatcacggcc atctgtgaaa ctatgcacat agacgcgctc gatctcgtgc atgtgcgcca
     1621 gttgcagcag cgcctctaag tgcgtctcgt gcgcgtggac gcccccattg cccagcagcc
     1681 cgcacaaatg cagttgcgag ccgttcttct tgacatgctc aaccgctttg agcagggccg
     1741 gattctggaa aaagctgcca tctttgatcg actcgctgac gcgcgtataa tcttgcagca
     1801 cacgcttgcc cgcgccgatg ttttgatgcc ctacctcaga gttgcccatc tgcccttccg
     1861 gcagccctac cgcctcgccc gacgtgcgta cagccgtgtg cggccactcc cgcgccagcc
     1921 ggtcgatatt gggtgtgcgc gccagggcaa tcgcgttgcc ctccttacgc gggttgatgc
     1981 cccagccatc catgataatc aacacaaacg gacgcggtcg cccaccagct cttgtcgcca
     2041 tatcttcttt caactccgtc tctcaattgt tctttgccca ggtcatgctg ttattgtatc
     2101 atcccgaccc ggtggttgat tgcctgcaac acactcacac tggatttaag ccacttaatt
     2161 ctgggatcta caagcaatgg tcttctgtag cgtggtacaa ttggcgtatg gacaaaatca
     2221 ctgctcaaaa caatggctca gtcaaagact acattgcttc actcgacgag caaacggtaa
     2281 aagatagcca agtgctgatt gaaatgatgc aacgaataag tgggcatgag ccaaagctgt
     2341 ggaatgtagg tacgattggg tttgatacct accattacaa atacgacagc gggcgcgaag
     2401 gcgacggtca tgttcttggc ttttatccaa gaaaagggaa gatcaccgtt tacctgatgg
     2461 acggtacggc acgctacgct gcattgctgg cgaagttagg gaaacatacg acgactgggt
     2521 attgtgtgta catcaagcgg ctcagcgatg tagagctgcc aatccttgag caaatcgtac
     2581 aacagtccta tgagtacatc aagtcaaagt cccaagacgg gcctctagac cgcatagtat
     2641 ggcaaactga aaaatagacg tttctgcctc aatggaactc ccgcctcttc ttttctgagt
     2701 atgtcctctt gatacggtat ctcaagtatt gctctccaac gggtaatctc atctctgatg
     2761 tggtggaaac ttttgcccac ttgctgcact aagaggaagc gatcttttta ttcggcgaga
     2821 catacgacac ctcgtcatat gatgcttgaa aggggtgcgc actcgaattg gacaaaaagg
     2881 cgtcatccgc gacgttttcg gcaatggatc gctgacagt
//
LOCUS       DASZAK010000024         2745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_320031, whole genome shotgun sequence.
ACCESSION   DASZAK010000024 DASZAK010000000
VERSION     DASZAK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320031"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..532)
                     /locus_tag="VGT44_00385"
     CDS             complement(65..532)
                     /locus_tag="VGT44_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579279.1"
                     /translation="MILLLRIGFGVLLGLTQFLLIFLYLRISAPIVWGSTPFILIITL
                     ILILFFIFPAFASVRPGLRLKQAWVGAVNGTVIGAVGALTLLVIVVTVLTIAVVTAVP
                     PTGTRIVIPARLVAAVLFAIALVVSLAGIVCAALGGWLGGAIGKLLAKWVKGE"
     gene            735..1904
                     /locus_tag="VGT44_00390"
     CDS             735..1904
                     /locus_tag="VGT44_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000009 - alpha-1,6-mannosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0004376 - glycolipid mannosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyltransferase family protein"
                     /protein_id="HEV2579280.1"
                     /translation="MKRAPKTDILWLFLGTRLLLVVLTYIGYILFPVPPHIYPSIPVD
                     VIGLLSSWNHWDAYNYLRIAQFGYQSVYDTAFFPLFPLLINGISFLFGHQGFAVIGMV
                     LSNLALLGTLFVLYQIAVDLFGEQVGRRTLLYLCIFPTAFFFFAAYNESLFLFLTASA
                     FLAMRRQKWWLVGLLGFLAALTRSAGLLLVIPYLYEVWLSRQGIFDRAGSAFSRLRQL
                     LPRVLPIILIPLGTSIYCIYCWVVYNNPLAFASVQNHWGRETTWPWVGIWGAFHALFF
                     VQPFGSFNEVHLLLDLSALIGFVTLAILGARKLRPSYSLWIALLLLYTFISPSEFQPD
                     TLISTQRFVLEMFPGFITLAALGIRHPRLHQSILLAFPFIQAALALLFILNRWMV"
     gene            2020..>2745
                     /locus_tag="VGT44_00395"
     CDS             2020..>2745
                     /locus_tag="VGT44_00395"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV2579281.1"
                     /translation="MRWLAFLSSRLIIALSQPLAAIISLQTLQNALSVVGYPAVTLFV
                     MIESSGIPFPGETMLLLASFFAATNHTLQIPFVIASAAFGAIIGDNIGFTVGRTGGRA
                     LVMRYGKYIFIKTEHLDRAEQFFTRHGNKTVFFGRFIAVLRAWAAFLAGVNHMRWRVF
                     LVYNAAGGIIWAAIYGTLGFLAGRYFHDNFGQVEQLASTIGWTGAIIIVAVATGLFIF
                     FYLRRRKHTHLAAASSVAETNQAH"
BASE COUNT          567 a          811 c          717 g          650 t
ORIGIN      
        1 gggggcggcg gggagcgggg tggagaccct tgcggtcgcc ctcgctgctc cgaccagaac
       61 cgcctcactc gcctttcacc cacttcgcaa gcaattttcc gatagctccg ccgagccagc
      121 cgccaagcgc ggcgcaaaca atgcccgcga gtgagacaac cagagctatg gcgaacaaaa
      181 cggcggcaac gagccgtgcc ggaataacta tccgcgttcc ggtaggcgga acggcagtaa
      241 cgacagcaat tgtcagcaca gtgaccacga taaccaacag cgtcaacgcg cctaccgcgc
      301 caataacagt gccgttgacc gcccccaccc acgcctgttt caagcgtaat ccagggcgca
      361 cactcgcaaa tgccggaaaa atgaagaaga gtatcaggat caacgtaatg atcagaatga
      421 aaggagtgga cccccatact ataggcgcgc taatcctgag gtacagaaaa atcagcaaga
      481 attgagtcag gccaagcaag actccaaagc caatacgtaa cagaaggatc attctcctcg
      541 tgcggatagg ccgcgattcc atccttcgta gcattatgtt gccctcccga aaatgtgcct
      601 ggtaagccag aacattcgtt agtatcatgt ggagagaata ttgacaccgc atatctcaga
      661 tgttgtacac atagtacgca tagcgggcag gagatttctc gtgaagtaag ctgctgtttt
      721 ccgggaacgc acctatgaaa cgagcgccaa agactgatat actctggctg tttcttggaa
      781 cacgcctgtt attggtcgtt ctgacctaca tcggctatat tttgtttccg gtcccgcctc
      841 acatctaccc gtccataccg gtagatgtaa tcggcttgct ctccagctgg aatcactggg
      901 atgcctacaa ctacctgcgt attgcgcaat tcggctacca gagcgtgtat gatacggcct
      961 tctttccgct ctttcccctg cttatcaacg gcatttcttt cctgtttgga caccagggat
     1021 ttgccgtgat cggcatggtg ctgagtaacc tggcgctgct cggcacattg tttgtgctgt
     1081 accagattgc ggtcgatttg ttcggcgaac aagtcgggcg gcgcacactg ctctacctgt
     1141 gcatctttcc aacggcgttc ttctttttcg cggcctacaa cgaatcgctt ttcctcttcc
     1201 tgaccgccag cgcatttctg gcgatgcggc ggcagaaatg gtggctggtc ggcctgctcg
     1261 gcttcctcgc cgcgctcacc cgctcggctg gcctcttgct ggtcataccg tacctgtacg
     1321 aagtctggct ttcgcggcag gggatcttcg accgcgcggg gagcgcattt agccgactgc
     1381 gacaactgct gcccagagta ctgccgatca tactcatccc actcggcaca tcgatctact
     1441 gcatctattg ctgggtggtc tacaacaatc cgctggcctt tgccagcgta cagaaccatt
     1501 ggggacgcga aacgacctgg ccctgggtgg ggatatgggg cgcgttccac gcgcttttct
     1561 tcgtccagcc attcgggtcg ttcaacgagg tacatctcct gctcgacctc agcgcgttga
     1621 tcggcttcgt cacgctggcc attctgggag cgcgcaagct gcggcccagt tattcgctct
     1681 ggatcgcgct gctcctgctc tacaccttca tcagcccttc cgaattccag cccgatacac
     1741 tgatttccac ccagcgcttc gtcttggaga tgttcccagg attcatcacc ctcgccgcgc
     1801 tgggcatacg ccacccgcgc ctgcaccaat cgatcttgct cgccttccca tttatacaag
     1861 ccgcgctggc gctgttattt atcctgaatc gctggatggt ctagggcagg taaaggtaat
     1921 agcccggttg cgccagctac cgattacgca tataatggcg cataagacag agatgtttcg
     1981 ttctgtgtca tggttgcgct cgcacgaaag gaaaacgata tgcgctggct cgcgttcctc
     2041 tcctcgcggc taatcatcgc gctgagtcag ccattggcgg cgatcatttc gttacaaacg
     2101 ctgcaaaatg cgttgagcgt agtagggtat ccggccgtga cgctttttgt gatgatcgaa
     2161 agttccggca ttccctttcc cggcgaaacg atgctgctgc tcgcatcctt ctttgccgcg
     2221 acgaatcata ccttgcagat accctttgtg attgccagtg cggctttcgg cgcgatcatc
     2281 ggcgataata tcggcttcac agttggccgc accggtggcc gcgcgctggt aatgcgctac
     2341 ggcaaatata tcttcatcaa gacggagcat cttgaccggg ccgaacaatt tttcaccagg
     2401 catggcaaca agaccgtttt ttttggccga tttatcgcgg tgctgcgcgc ctgggccgcg
     2461 ttcctggcgg gcgttaacca catgcgatgg cgcgtcttcc tggtctataa cgcggcaggc
     2521 ggcatcatct gggccgccat ttacggcacg ctgggttttc tggcagggcg atattttcac
     2581 gataattttg ggcaagtcga acagctcgcc tccacgattg gctggacagg cgcaatcatc
     2641 atcgtcgctg tcgccaccgg tctattcatc tttttctacc tgcggcgcag aaaacataca
     2701 cacctggcag cggcttccag tgtagctgag acgaatcagg cacat
//
LOCUS       DASZAK010000025         2050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_390944, whole genome shotgun sequence.
ACCESSION   DASZAK010000025 DASZAK010000000
VERSION     DASZAK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390944"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..920
                     /locus_tag="VGT44_00400"
     CDS             96..920
                     /locus_tag="VGT44_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579282.1"
                     /translation="MTQTQSTEEKARQKKQWTDLAIQQAQAGLWAEAVETNQNILNLF
                     PFEPDAYNRLGKAYNELGQYREARQAYTQTLKYSPKNAIAKKNLDRLSQLTDEPAQAP
                     QVHSAGADRIDPRLFIEEPGKTGTTDLDNVSVGVLAKVGVGDRVQLHVNGHTIMVRNA
                     SGEDIGQIKPILANRLIKFMEGGNRYAAAILAIENGAVRLMIREEYQHPSMFGRVSFP
                     SQGGGETIRAYTKDSLLRYDRDDEEEVGSDDEYFDGGDEAEEMTEIDFEGGLESEE"
     gene            1124..>2050
                     /locus_tag="VGT44_00405"
     CDS             1124..>2050
                     /locus_tag="VGT44_00405"
                     /inference="COORDINATES: protein motif:HMM:NF019520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HEV2579283.1"
                     /translation="MNATNVMSDLHSPIPSASVRNILTLLAPRGAELVAGGEGLHRRV
                     TWACRMRARLPAFEAIRGGELALLTLAQLRRLDETLPHLLASLHREGVAAVAVASPTL
                     ELLGQEASVLADQLHLPLLLLPLTASLEEIEREVITFVVGFRGESERKAAEVSRTLMQ
                     LSAQGAGVAGLCDHLARVCAKWVMLQDAGHEVLYQSIPPDMQHLTLPNRLTDDLLRKQ
                     GLERAVVPVLIRHEVMGYLSLLGGEGDFDYLERMVLGQAAPIVALEFARERERSEVEG
                     RYAAEALMDVLQGNYSLAEEMVARGRLLGFDLT"
BASE COUNT          486 a          555 c          627 g          382 t
ORIGIN      
        1 atcacaccac ggcctggtcg ttgcagggtt gcgcggttga gttataatat gcagaaattg
       61 cacagatctc tctgtaagga tagaggaggg tttcattgac gcaaacacaa tcgactgagg
      121 aaaaagcgcg acagaaaaaa caatggacag acctggcgat ccagcaggca caggccggcc
      181 tgtgggcaga ggcagtcgag accaatcaga atatcttgaa tctgttcccg ttcgagcctg
      241 atgcctataa tcgcctgggc aaagcctata acgagttggg ccagtataga gaggcgcggc
      301 aggcgtatac gcagacgctc aagtatagtc caaagaatgc tatcgccaaa aagaacctgg
      361 acaggctttc tcagttgacc gatgagccgg cacaagcgcc gcaggttcac agcgcggggg
      421 cggatcggat tgatcccagg ctctttatcg aagagccggg taagaccggc acgaccgacc
      481 tcgataatgt ttcggtagga gtcctggcga aagtgggcgt gggcgatcgc gtacaattgc
      541 acgtgaatgg ccataccatc atggtgcgca acgcttccgg cgaggatatc gggcaaatca
      601 agccgatcct ggcaaatcgc ctgatcaagt tcatggaggg cggcaatcgc tacgctgcgg
      661 ccatcctggc catcgaaaat ggcgcggtgc gcttgatgat ccgtgaagag taccagcatc
      721 cgagcatgtt tggcagagtc agcttcccat cgcagggcgg cggcgaaacc attcgtgcgt
      781 atacaaaaga tagtctgctg cgctacgacc gcgatgacga ggaagaggtg ggcagcgacg
      841 acgaatactt cgacggcggc gacgaggctg aggagatgac cgagatcgac ttcgagggcg
      901 gcctcgaaag cgaggaataa tgtcgccgaa catatgaaaa tacatcaaac aggaaggagt
      961 aaagcgaaca catgaataag acaaaacgag tggcatggcg caaacatcgt atcaaagcca
     1021 aaaaatacga tgaaaagcgc aaagcacaga aggccagtgg caccgtagcc acctcgacag
     1081 cgcggcgata atcgccgcgc ttccctgttt ttaggaaccc gcgatgaacg caaccaatgt
     1141 gatgagtgac ttacattctc ccataccctc tgcatccgtc cgaaatattc tgacccttct
     1201 ggctccacgt ggagcagagc tggttgccgg aggtgaggga ttacatcgcc gggtcacatg
     1261 ggcgtgtagg atgcgcgcgc gcctgcctgc gttcgaggcc attcgcggag gagagctggc
     1321 cctgctgacg ctggcgcagt tgcgcagact ggatgagaca ctgccacacc tgcttgccag
     1381 cctgcaccgc gaaggcgtcg cggctgtcgc ggtcgcgtcg cccacgctcg aattgctggg
     1441 ccaggaagcg tctgtgcttg ctgaccagtt acaccttccc ctactgctgc tgcccctgac
     1501 ggcctcactt gaggagatcg aacgcgaagt aatcaccttc gtcgtaggct ttcgcggcga
     1561 gagcgagcgc aaagctgccg aggtctcgcg caccctcatg cagttgagtg cgcagggggc
     1621 gggcgttgcc gggctatgcg atcaccttgc gcgcgtctgc gctaaatggg tcatgttgca
     1681 ggatgccgga cacgaggtcc tttaccagtc gatacctccc gacatgcagc atttgaccct
     1741 gccgaaccgc ctgacagacg atctattgcg caagcaagga ctggaacgag cggtcgtgcc
     1801 ggtactcatt cgccacgagg tgatgggata cctgtcgttg ctcggcggcg aaggcgattt
     1861 cgactatctt gaacgcatgg ttctcggcca ggccgcgccc atcgtggcgc tagaattcgc
     1921 acgcgagcgc gagcgcagcg aagtcgaagg ccgttatgcc gccgaagcct tgatggatgt
     1981 cctgcaagga aattattcgc tcgcggagga aatggtcgcg cgagggcgtc tgctcggatt
     2041 cgacctgacg
//
LOCUS       DASZAK010000026         9840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_142482, whole genome shotgun sequence.
ACCESSION   DASZAK010000026 DASZAK010000000
VERSION     DASZAK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142482"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1392)
                     /gene="ahcY"
                     /locus_tag="VGT44_00410"
     CDS             complement(124..1392)
                     /gene="ahcY"
                     /locus_tag="VGT44_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEV2579284.1"
                     /translation="MTTSTKGDVKDLFLAARGKDRIEWAAKEMPVLRLIRDRFASDQP
                     LKGVRMSGCLHITTETANLAITLKAGGADLVLCASNPLSTQDDVAAALIAEYGIPTYA
                     IKGENEETYYRHIHSVLDHQPNMTMDDGCDLVSTLHTSRTELIPAIIGGMEETTTGVI
                     RLHSMEKEGVLRFPVLAVNESDTKHLFDNRYGTGQSSIDAIIRSTNVLLAGKTFVVFG
                     YGWCGRGVASRAHGMGANVIVCEVDPTRALEAVMDGYRVMPGIEAAALGDIFITVTGN
                     INVLDRQHLERMKDGALLANSGHFNDEINIPALESLSTAKRRVRDFVDEYTYADGRKV
                     HLLAEGRLVNLSAAEGQPASVMDMSFANQALGAEYMLQNAQQLQPRVFTIPAEIDKEI
                     ARLKLHAMGVRIDTLTGEQDTYLNSWESGT"
     gene            complement(1469..2410)
                     /locus_tag="VGT44_00415"
     CDS             complement(1469..2410)
                     /locus_tag="VGT44_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV2579285.1"
                     /translation="MSILVVGSVALDTIETPFGGAEEILGGAASFFTTAASLYSQVNL
                     VAIVGSDFPQEHLDFWRKRPVDLKGLQIEVGRTFRWVGRYHMDMNSRDTLDTQLGVYA
                     GFHPVIPAEYARSDLLFLANIQPELQMEVLEQVPNARLTVLDTMELWIMTEREQLTEV
                     MRRVDMLLMSEEEVRQFTGRSSILAGVRQLLELGLKYVVVKQGSYGALLFGADGAYFS
                     APTYPLEEVVDPTGAGDAFAGGLMGYLSTVAPAPGGRYAIDEIKRGIVHGNIIGSFAC
                     EDFSIGRLRTLTMDDVAARYQALVTCSHFDPHWQSPS"
     gene            complement(2440..3090)
                     /locus_tag="VGT44_00420"
     CDS             complement(2440..3090)
                     /locus_tag="VGT44_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2579286.1"
                     /translation="MDSAIVQAAAVYAEQTLGKEAFLALSAVVATCKMLALALAEQVE
                     EGEAREVLEAPDLDALVTAAYLHNISVAEFGYQAHHQKSAQMAVEFLRKLDVPDDRMQ
                     RVERAILLHTSATSNEERASGPPEGRILYDADKLGRLSGLSVVTSLIEFGARYPNLTV
                     TNEVLVAILRHVEERFVNLYQSLNTGPARRMAQVKFGNTLAFLDGVIEYLDDATPI"
     gene            complement(3090..4211)
                     /gene="mtnA"
                     /locus_tag="VGT44_00425"
     CDS             complement(3090..4211)
                     /gene="mtnA"
                     /locus_tag="VGT44_00425"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HEV2579287.1"
                     /translation="MAGEQESGELRTVWWQALVEGEAVCMIDQARLPGEEVTLTLTTE
                     QQVAKAIATLQVRGAPAIGVTAALGMALAATRFRRERGDTAPPGELVEHLLGVAELLR
                     ATRPTAVNLSWGVERTLRCAERASREQLSTAELVSRVIAEAKAVADEDYEACLRMGEF
                     GAQLIADGDTLLTHCNAGALATSGLGSGLAPVYVAHRAGKRLHIFVDETRPVLQGARL
                     TAWELQRAGIPLTLITDNMAAHFMRHGGIKAVFVGADRIAANGDVANKIGTYSVAVLA
                     RAHDIPFYVVAPRSTIDLRLSSGELIPIEQRNPDEVTTLRGVPIAPAGVQVANPAFDV
                     TPHTYVTAIITENGIARAPYVDALRALCVGYHSREKADV"
     gene            4528..5817
                     /locus_tag="VGT44_00430"
     CDS             4528..5817
                     /locus_tag="VGT44_00430"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV2579288.1"
                     /translation="MEAQTDVQTYIERGKQALAQGQGREAAIAFAHAAQIEPNNPYIH
                     LGLAEANLALGSYGVVYMACRKVQELQSEGGTASTMAQALLDLLDRRYDRALQNIETV
                     INDDPANAYAHAMRSYLLRLTGQDYDAGLARSRASRLSYGGTFENVFPPVDPARTPGY
                     KGQGSTPPVVDADDPNAATFTRDRNGTQNGAQIEGDQVPGWTRQRPGGMSEMQRRRVR
                     ARFWMSQNPRFVTNILIGINVLVFLISVLLTITQTGTLGGLGDVDQNILINMGAQWNL
                     LVSQGQWWRIFTAMFLHLNLLHIGLNMLSLFFIGTAIEMFYGKWRYLTIYMLSGIVGG
                     IVSYFVEGPGFLAVGASGAIFGVFGALGVFYFVNRRALGRYGSGAITNWVFWLALNLV
                     FGFSNPQIGIVDHLGGLVAGMVIALLLIPRLRKPRVV"
     gene            complement(6139..7239)
                     /locus_tag="VGT44_00435"
     CDS             complement(6139..7239)
                     /locus_tag="VGT44_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579289.1"
                     /translation="MILKDRLGLFALALVAVGIAFFLAPGGQLQGVGGIVFGTGLTVF
                     LGRLTNRQQLAKEANLRRKTEVYEPLYIELQTLRKRLEEARAGTKPYLQQIDVPGQAS
                     LPQLEEPPRLHRWSEFKADSRYLEFSEAIRRMLDQTLELAKEYNASVEAALKDFEAIL
                     ATYIETAINSTVHHPDFQRWRRSSPDVRSLSPDNAWFTQIHSVLELEQSKSPYHVVLL
                     PEFIDPEFEPAYDKYITEVARAWATSWLRGWGAVEHKSRAPLDMAVPFPRQATPRPAT
                     LGWLLAGDCTQAALEVIRTADFLLSPPLPPIERLRDIMYDTWLALGKLEIYQEVRSRH
                     RELFDQVSTVERKFKDKLEYIQTIYEGGPPLL"
     gene            7482..7637
                     /locus_tag="VGT44_00440"
     CDS             7482..7637
                     /locus_tag="VGT44_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579290.1"
                     /translation="MPDLLAIALQALFSKLSAIRRKTLSNRVTGGSIDLVHGRWLIAE
                     ELSYLFQ"
     gene            complement(7943..8734)
                     /locus_tag="VGT44_00445"
     CDS             complement(7943..8734)
                     /locus_tag="VGT44_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2579291.1"
                     /translation="MLLNNRYELSDQAGRDATSTVYRGIDTYTGKSVAIKVLRPIYST
                     DAKRTIPFHNSAKLSIALQHPNIVQVLDYGQDDENYYIVREFVEGIDLRHYFRSRGGI
                     LDINRVIIIAGSVASALGYAHDREIVHGDLKPQNILVVRGMHPPVKVADFGMSSIGVT
                     LGAIQYRSPEQLQAETVSPGPATDVYSLGILMYEMMVGYTPFDGDTPVSVAIQHMHEI
                     PIPPTIFNPNIPQSLEGIVMKCLEKEPEKRLKNGVELTRALEGII"
     gene            complement(8828..9682)
                     /locus_tag="VGT44_00450"
     CDS             complement(8828..9682)
                     /locus_tag="VGT44_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579292.1"
                     /translation="MDENVQQKITLACVALLQKNAADSQQTRKRTDRRSPTTKKEAIE
                     LLLLAASEPDITLEHVTDALWIIYKRSKPASQTRQWVTHLLFDLVRQADIIANDAVDV
                     ATAFYVLSPQSSREQQEAAQQLLALAKRRDIPFGETVEAAHSLYVQSPHGSQERQQAI
                     EMLLAQAHWPDTTAAQAQEAVLALCYASAYRSKERKLAIQALIEVIRRPGLSFEDAVV
                     LDDERAIIGSTRALHQQQWAAKKQMWETIGQRADLTSEQHEQVVTALEEYRRLLTRFP
                     LPVSDASS"
BASE COUNT         2112 a         2901 c         2588 g         2239 t
ORIGIN      
        1 ttctgtacct gctttccatg ctagataacg acgtaactct tgccgtcgcc ctggcagtac
       61 ttaaacagcg acaagcctgg gctggctcgc cagcccaggc ttgtcgctgt ttaagcttat
      121 aacctacgtc cccgactccc acgaattcag atacgtatcc tgctcacccg tcagggtatc
      181 gatacgcaca cccatggcgt gaagcttcaa gcgggcaatc tccttgtcga tctcggcggg
      241 gatggtgaag acgcgcggct gcaactgctg cgcgttttgc agcatgtatt ccgcgccgag
      301 cgcctggttc gcgaagctca tatccatcac gctggcgggc tgtccttcag cggcggagag
      361 attgaccagg cggccctcgg ccagcaggtg aaccttgcgg ccatcggcat aggtgtattc
      421 atccacaaaa tcgcgcacgc gccttttggc agtggacagg ctctccagcg ccggaatgtt
      481 gatctcgtcg ttgaaatggc ccgaattggc gagcagcgcg ccatccttca tgcgctccag
      541 gtgctggcga tccagcacat tgatgttgcc cgtcaccgtg ataaagatgt cgcccagggc
      601 ggcagcttcg atgcccggca taacgcggta gccatccatg acggcctcca gggcgcgcgt
      661 cgggtcaacc tcgcacacga tcacgttggc tcccatgccg tgcgcgcgcg aggccacgcc
      721 gcgaccgcac caaccgtagc cgaagacgac gaaggtcttg ccggccagca gcacgttggt
      781 cgagcggatg atggcatcta tggaactctg ccccgtgcca tagcggttat cgaagaggtg
      841 cttcgtatcg gactcgttga cggccagcac ggggaagcgc agtacgccct ctttctccat
      901 gctgtgcagg cggatcacgc cggtcgtcgt ctcttccatg ccgccgatga tggcgggaat
      961 cagctccgtg cggctggtat gcagcgttga gaccaggtcg cagccgtcat ccatcgtcat
     1021 attgggctgg tgatccagga cggagtgaat gtggcgataa taggtctctt cgttctcacc
     1081 cttgatcgca taggtcggga tgccatattc agcgatcaac gcggcggcca cgtcatcctg
     1141 cgtcgagagt gggttggacg cgcacagcac caggtcagcg ccacccgctt tgagcgtgat
     1201 ggcaaggttg gcggtctcgg ttgtgatatg caaacagcca gacatacgca ctcccttgag
     1261 gggctggtcg ctggcgaaac ggtcacggat cagccgcagc accggcattt ctttcgcggc
     1321 ccattcaatg cggtctttgc cgcgcgccgc caggaagagg tcttttacat cccccttcgt
     1381 cgaagtcgtc ataagtgtcg tgttcctttc cggcaacagc aatgtgccga tgtgattctc
     1441 aaatacaaac cgttatatcg taccatccct acgacggcga ttgccagtgt gggtcgaaat
     1501 gcgagcaagt gaccagggcc tggtagcgcg cggccacatc atccatcgtg agggtgcgca
     1561 ggcgtccaat actgaaatct tcgcaggcga acgaaccgat gatgttgcca tgcactatgc
     1621 cgcgcttgat ctcgtcgatg gcatagcggc caccgggcgc gggcgcgacg gttgagagat
     1681 agcccatcag gccgccggca aaggcatccc ccgcgccggt aggatcgacg acctcttcga
     1741 gcggataggt tggggcgctg aaataggcgc cgtccgcgcc gaacagtaac gcgccgtagc
     1801 tgccctgttt gaccaccacg tacttcaagc caagttcaag cagctggcgc acaccggcaa
     1861 gaatgctgga acgcccggta aactggcgca cctcctcttc gctcatcagc agcatatcaa
     1921 cgcgccgcat gacctccgtg agctgctcgc gctccgtcat gatccatagc tccatcgtgt
     1981 ccagtacggt gaggcgcgcg ttcggcacct gctcaagcac ctccatctgc aattccggct
     2041 ggatattggc tagaaagagc agatcgctcc tggcatactc ggcgggaata acgggatgaa
     2101 agccagcgta cacaccaagt tgtgtatcga gcgtatcgcg cgagttcata tccatgtgat
     2161 agcgcccgac ccaacggaag gtgcgcccca cttcgatttg caatcccttc agatcgacag
     2221 gccgtttacg ccagaaatcc aggtgttcct gggggaaatc gctgccgaca atggccacga
     2281 ggttgacctg gctataaagc gaagccgccg tcgtaaaaaa agaagcagcc ccgcccagaa
     2341 tctcctcagc gccgccaaag ggggtctcga tggtatcgag cgccacagaa ccaacaacca
     2401 gaatggacaa gctaccgtcc tccatttcaa agtaactgtt cagatgggtg tggcgtcgtc
     2461 aagatactca atcacaccat cgagaaatgc cagcgtattg ccgaacttga cctgcgccat
     2521 gcgccgggcc ggaccggtat tcaacgattg atacaggttg acgaaacgct cttcaacatg
     2581 tcgcaagatg gcgaccagta cctcgttcgt aacggtcaga ttgggatagc gcgccccaaa
     2641 ttcgatcagc gacgtgacca ccgacaggcc actcaaccgc cccagcttat cggcatcata
     2701 gagaatgcgg ccttcgggcg gtccggacgc gcgttcctcg ttcgatgtcg cggacgtatg
     2761 cagcaggatg gcgcgttcga ctctctgcat acgatcatcg ggtacgtcca gtttccgtaa
     2821 aaattcgaca gccatctgcg cggacttctg gtgatgcgcc tggtagccga actcagcaac
     2881 cgagatatta tgcaggtagg ctgccgttac cagggcatcc agatctggcg cttccaatac
     2941 ttcgcgcgct tcgccttcct caacctgctc cgccagtgcg agcgcgagca tcttgcaggt
     3001 ggcgacgacg gctgagagcg ccagaaatgc ttctttgccc agcgtctgtt cagcgtagac
     3061 ggcggcggct tgcacgatgg cggaatccac tagacatcag ctttctcacg actatgataa
     3121 ccgacgcaca acgcgcgcag ggcatccacg tagggcgcgc gcgcaatgcc attttcagta
     3181 attatagcgg ttacataggt gtgaggggta acatcaaagg ctggattggc tacctgcacc
     3241 ccggcgggag caatagggac gccgcgcagc gttgtcacct cgtctggatt gcgctgctcg
     3301 atcggaatta gctcgcccga cgacagccgc aggtcaattg tcgagcgagg cgcaacgacg
     3361 taaaacggaa tgtcgtgcgc gcgcgccagc accgccacgc tgtacgtgcc aatcttgttg
     3421 gcgacatcgc cattggccgc gatacggtcc gcgccgacga agaccgcctt gatgccgccg
     3481 tgccgcataa aatgcgccgc catgttgtca gtaatcaatg tgagcgggat gcccgctcgc
     3541 tgcaattccc aggcggtcag ccgcgcgccc tgcaacaccg ggcgcgtctc atcaacaaag
     3601 atgtgcaggc gtttccctgc cctgtgcgcc acgtatacgg gcgcgagccc cgaccccagg
     3661 ccggaggttg ccagcgctcc cgcgttgcaa tgggtcagca gcgtatcccc atcggcaatc
     3721 agctgcgcgc cgaactcgcc catgcgcagg cacgcttcgt aatcctcgtc agcgacggcc
     3781 ttcgcctccg ctatgacccg cgacaccagt tcagccgtcg aaagctgctc acgagaggcg
     3841 cgctcggcac agcgcagggt gcgttctacc ccccaggaca ggttgaccgc cgtcgggcgc
     3901 gtcgcgcgca gcagttcggc cacgcccagc agatgctcaa caagctcacc gggcggcgcg
     3961 gtatctcctc gctctcgcct gaaacgggta gccgccagcg ccatgcctaa cgccgctgtc
     4021 acgccgatgg ccggcgcgcc gcgcacttgc agcgtcgcaa tcgccttagc cacctgctgc
     4081 tcggttgtca gagtcagcgt cacctcctcg cccggcagcc gcgcctggtc gatcatgcag
     4141 accgcttcgc cctcgacaag cgcctgccac cagaccgtgc gcagctcgcc gctctcctgc
     4201 tctcctgcca tgccgcgttg gaactccttc tttgaatatg ggaaacacac ctgcatggta
     4261 tcataccacc tcgtcaaggt tcaaacgatc ggggttgttc cactcttccc gcttgcttgc
     4321 gcccctacac gtatttccta cgctccgcgc aggcataggc aaagcttgct acatacgata
     4381 taatcacaac acacaagatc accagcttgt caggcaacca atcgtacact ctatcgtatt
     4441 acaagataga gacacatatt ttgatctgga cttatcttaa tcgtatcgct accctatcgc
     4501 gctactttag tatgggaggt cacattcttg gaagctcaga ccgatgtaca aacgtacatt
     4561 gaacgtggaa aacaggcgct tgcgcagggc caggggcgag aagccgccat tgccttcgcg
     4621 cacgccgctc aaatagaacc aaataatccc tacattcacc tgggactggc ggaggccaat
     4681 ctcgcgctcg gctcctacgg cgtggtctac atggcctgcc gcaaggtgca ggaactccag
     4741 tccgaaggcg gcaccgcttc gacaatggcc caggccctgc tcgacctgct cgaccgtcgc
     4801 tacgatcgcg ccctgcaaaa catcgaaacg gtcatcaatg acgatccggc caatgcctac
     4861 gcccatgcca tgcgctccta cctgctgcgc ctcaccggcc aggactacga cgcaggtctg
     4921 gcgcgctcgc gcgcatcacg tctctcctac ggcggcacct tcgagaacgt cttcccgccc
     4981 gtcgatcccg cgcgcacacc cggctacaaa ggtcagggca gcacaccgcc agttgtcgat
     5041 gccgatgacc ccaatgccgc gacatttaca cgcgaccgta acggcacaca gaatggtgcg
     5101 cagatcgaag gcgaccaggt ccccggctgg acgcgccagc gccccggcgg catgagcgag
     5161 atgcaacgcc gccgcgtccg cgcgcgcttc tggatgagtc agaacccacg cttcgtcacc
     5221 aacatcctca tcggcatcaa cgtacttgtc ttcctgatct ccgtgctgct caccatcacc
     5281 cagacaggca cattgggcgg acttggcgat gtcgaccaga acatcctcat caacatgggc
     5341 gcgcagtgga atctccttgt atctcaaggc caatggtggc gcatcttcac cgccatgttc
     5401 ctgcacctca acctgctgca catcggcctg aatatgctct cgctcttctt catcggcacc
     5461 gccatcgaaa tgttctacgg caaatggcgc tacttgacga tctatatgct gtccggcatt
     5521 gtcggcggca tcgtctcgta cttcgttgag gggcctggat tcctggccgt cggcgcgtct
     5581 ggcgcgatct tcggcgtctt cggcgcgctc ggcgtcttct acttcgtcaa ccgccgtgcc
     5641 ctgggccgct atgggtcggg cgccatcacc aactgggtct tctggctggc gctgaacctg
     5701 gtcttcggct tttccaatcc acaaatcggc atcgtcgatc acctcggcgg cctggtcgcc
     5761 ggtatggtga tcgcgctgct cctcattcca cgcctgcgca agccgcgcgt cgtctaaccc
     5821 tacaccacga tgagccaggg caaccacgag aattatcctg gctcatcgtg gtgtagtttg
     5881 atacatacac atcttgttta ttgtgctggt cagtacactg aaaagtagga agtcgagctt
     5941 gcaaatctcg ctgtgacccg gctgattgag tcacactgat cctatccatg ccctttaccc
     6001 ccctgctcga cttcctattt tttgcccctt tgtgtgacac aaggcataaa atggccgcca
     6061 cggccatgaa ttgctttttt tgggaaattg tccgatgcgt tttctgcgcc aactcttgtc
     6121 tagctgtccg cctggtctct acagcagagg tggtcctccc tcgtagatag tttgaatgta
     6181 ctccaatttg tctttaaact tccgttctac cgtacttact tgatcgaaca attcgcggtg
     6241 acgcgaacgc acttcctgat atatttcaag ttttcctaga gctagccatg tatcgtacat
     6301 gatgtctctt aatcgctcaa tgggtggaag cggtggagaa agtagaaagt cagcagtacg
     6361 gataacttct agtgctgctt gtgtgcaatc tcctgctaaa agccaaccaa gcgttgccgg
     6421 tcgaggtgtt gcttgccggg gaaatggtac agccatatct aaaggcgccc gagatttgtg
     6481 ttccaccgct ccccaccctc tcagccagga cgttgcccat gcccgtgcta cctcagtaat
     6541 atatttgtcg taagcaggct caaactcagg atcaataaac tccggtagca aaaccacgtg
     6601 atacggtgat ttagattgct caagttccag tacactgtga atctgtgtaa accaggcgtt
     6661 gtcaggcgag agagatctga catcagggga tgaccggcgc catcgctgaa agtcagggtg
     6721 gtgtactgtg ctgttgatgg ctgtttcgat atatgtggca aggatagctt caaagtcttt
     6781 caaagccgct tcgacagagg cattgtattc cttagcgagc tcaagcgtct gatcgagcat
     6841 tcgacggatt gcctctgaaa attccagata gcgtgaatca gccttgaact cggaccaacg
     6901 atgcaggcga ggaggttctt cgagttgggg aagggaagcc tgtccgggta catcgatctg
     6961 ttgaaggtac ggcttcgttc ctgcacgcgc ttcttcaagc cgcttacgga gagtttgtag
     7021 ttcaatatat aaaggctcat agacctctgt tttgcgtcgc aaattggcct ctttcgccaa
     7081 ctgttgccgg ttggttaatc tcccaagaaa gaccgtcagg ccagtcccaa agacaatacc
     7141 tcccacccct tgtaattggc ctccaggagc aagaaagaaa gcgatcccaa cggcaacaag
     7201 ggccagcgca aataatccca aacgatcttt gaggatcata tgacctccac ccagcacacg
     7261 ctagctaagc agcttctact tgcgctggaa tacgactcag acaagcctgc ggagacgcat
     7321 gttcaccgtg acgtttcctc attccctgtc tccagctcgt gacaaactgc tcgaagaagc
     7381 gctcgtacct cgggatcgcg caaaatggcc ggttgcacga gataccgagc gatatgccgc
     7441 tgcgtcagcg ccacttgctc tgcccgaagg agtagcgcaa tttgcctgat ctcctcgcga
     7501 tagccttgca agcgcttttc tcgaagctct cggcgatacg ccgtaaaacg ctcagcaatc
     7561 gcgtgaccgg cggctcgatt gatcttgtac atgggcgttg gttgatagcc gaggagcttt
     7621 cctacctctt tcagtaaggg agacggtttt tcggctcggc ttgctgcctg ctccaactga
     7681 tgggaaatgc tctcctttta catgtatctc cctatccacc aaatcatatc acgcaaaaag
     7741 gattcccggc caagttatgc ccaattgttg cactttcggc caggaaatgc cgttacgagg
     7801 ggggtgttat ggcacctcga gcgtaaaagt ttatacatgc tgccgagata tgcttttttg
     7861 aacaaattcc tcaacgagtt tttaggtgtg ttcccctgtt ctggtgaagc tgccctgtgg
     7921 ttaggtacgg ggatgtgata ttctagatta tgccctctaa ggctcttgtt aactcaaccc
     7981 cattcttcaa gcgcttctca ggctcttttt ccaaacactt cataacaatt ccttctagag
     8041 attgtggaat gttagggtta aaaatcgtcg gtggtattgg tatctcgtgc atatgctgta
     8101 ttgcaaccga gacaggcgta tcaccatcaa acggtgtata acctaccatc atctcataca
     8161 tgagaattcc aagtgagtaa acatcagtag caggaccagg actaaccgtt tccgcctgta
     8221 gttgttctgg cgaacggtat tgtattgccc caagtgttac gccgatagaa ctcattccaa
     8281 agtcagcaac cttaacgggg ggatgcatac ctcgtacaac tagaatgttt tgaggtttca
     8341 aatcaccgtg tacaatttcc ctatcgtgtg cataccctaa agcagatgca acactaccgg
     8401 caataatgat gactctgttt atgtccagaa tgccaccacg cgatctaaag taatggcgta
     8461 gatcaattcc ctctacaaac tccctaacga tatagtagtt ctcatcatcc tgtccatagt
     8521 ctaagacttg tacaatgttg ggatgctgca atgcaatcga taacttagct gaattgtgaa
     8581 atggaattgt ccgttttgca tctgtgctat aaatagggcg gagaactttg attgctacag
     8641 acttgccggt atacgtgtct atacctctgt agaccgttga tgtagcgtca cgaccggcct
     8701 ggtctgatag ttcataacga ttattcaaaa gcattactca tttctcctga cacgaatgta
     8761 tacaatgcaa gaaattccta ccctcctggt agacgataat agcacgtaaa gaagttcgat
     8821 acaactatta agaagaagca tctgatacag ggagaggaaa gcgagttagg agccgtcgat
     8881 attcttcaag agccgtgacg acctgctcgt gctgctcgga ggtcaggtcg gcgcgctgcc
     8941 cgatggtctc ccacatctgc tttttcgccg cccattgttg ttggtgtaat gctctggtac
     9001 tgcctatgat ggctctctcg tcatcgagga cgacggcatc ctcaaatgag agtcccggtc
     9061 gcctgatcac ttctatcagc gcctggatgg ccagcttgcg ctccttcgac cggtatgcac
     9121 tggcataaca caacgccaat acggcctctt gcgcttgcgc tgcggtggtg tccggccagt
     9181 gagcctgtgc cagcaacatc tcgatagcct gctggcgctc ttgtgacccg tgaggacttt
     9241 gcacgtaaag tgagtgggca gcttctacgg tctccccgaa aggaatatcc ctgcgctttg
     9301 ccagcgccag caattgctgg gcagcctctt gctgctctcg tgagctctgg ggacttaata
     9361 catagaatgc ggtggcaacg tctacagcgt cattggcgat aatatctgct tgtcgcacca
     9421 ggtcgaacaa caggtgcgtc acccactgcc ttgtctgcga cgctggctta ctcctcttat
     9481 agatgatcca gagtgcgtcc gtgacatgct caagggtgat atctggctcg cttgccgcta
     9541 aaagcagcaa ttcgatcgct tccttttttg ttgttggcga gcgtcggtcg gtgcgttttc
     9601 ttgtctgctg ggagtctgct gcgttcttct ggagaagggc aacacaggcc agagtgatct
     9661 tctgttgtac gttttcatcc attaatgtcc tccagaggca tatcgaagcg gtcagaaaag
     9721 tgtgattcaa tggtagaacg ttcaagcaca cggtttttcg gtagatgcct cgttcgatga
     9781 gtaatcatcc ccttgacctt tacgtaacgt catgctttat acttcctcca tagaaaggag
//
LOCUS       DASZAK010000027         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_320061, whole genome shotgun sequence.
ACCESSION   DASZAK010000027 DASZAK010000000
VERSION     DASZAK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320061"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2007)
                     /locus_tag="VGT44_00455"
     CDS             complement(<1..>2007)
                     /locus_tag="VGT44_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEV2579293.1"
                     /translation="TTAALARDDLPPGASLRELGTHRLKDLQRAEPLFQLVHPDLPAE
                     FPPLRSLEAFAHNLPVQWTSFIGREREMAEVRRLLRATRLLTLTGTGGIGKTRLSFQI
                     AREMQPHLADGICFVHLAAVSETDLVIPRIAQELGIQEIGGQPILEAIKASLSKKEFL
                     LLLDNFEQVVETAPLIEDLLIACPRLKVVVTSRATLNLDAEQVFHVPPLAFPSDMKML
                     TQEELLTQYAAIALFVQRARAIASFQMTQTNAKSIAEICKRLDGLPLAIELAAARMKI
                     LSPEALLAKLDEPLKTLTSQSRTLPLRHQTLRNAIQWSYDLLDAGEQRMFRRLSVFRG
                     ALVIEAVEAMQQAMNEVDNVPVIDRLASLLDKSLLYRSQIEGMEPYIQMLLTVREYAL
                     DLLQESGEFEQARRAHAMHYLNLAEQADLHLKGPQQLKWLAQLEMGQENLWVALAWCM
                     EHIGYAEVEETALRLCGALGWFWFLRGYWSEARRWLDGILGADSSSEATAARAKARYV
                     SGMLAYQQDDYVQARPLLEESRRIYDWLGMKREYANALGTLGQLTYLQGDLDTAWSML
                     KESETLCRSLDDAWGLISALRRLGYVMLGENDLAQALVNIQEGLELARQLGDKYLLAI
                     LLLTQGDIAASQSDLAQAVALDQEGLGFARELGHKSLIALAMQNL"
BASE COUNT          377 a          638 c          576 g          416 t
ORIGIN      
        1 caggttctgc atcgccaggg cgatcagcga tttgtgtccc agttcgcgcg caaaccccag
       61 tccctcctgg tcgagcgcga cggcctgcgc caggtcgctc tgcgaggccg cgatatctcc
      121 ctgggtcaac aggagaatgg ccaggagata cttgtcgccc agttgacggg cgagctccag
      181 cccctcctga atgttaacca acgcctgcgc caggtcgttt tcgccgagca tgacatagcc
      241 aagcctgcgc agggccgaga tcagccccca cgcatcatcc aatgagcggc agagcgtctc
      301 gctctctttg agcatcgacc aggccgtgtc caggtcgcct tgcaagtagg taagctgtcc
      361 taatgtaccc agggcgttgg cgtattcgcg cttcataccc agccagtcat agatgcgtct
      421 gctttcttcc agcagagggc gcgcctgtac gtagtcatcc tgctggtagg cgagcatccc
      481 tgatacatac cgcgcctttg cgcgtgctgc cgtcgcctcg cttgagctgt cagcccccag
      541 aatgccatcc agccagcgcc gcgcttcgct ccagtatcca cgtaagaacc agaaccagcc
      601 taaagctcca cagagtcgca gggcagtttc ctccacctct gcatatccga tatgctccat
      661 gcaccatgcc agcgctaccc acaagttttc ctgtcccatc tccaattgcg ccagccactt
      721 gagctgttgc ggacctttga ggtgcaggtc tgcttgctcg gccaggttca ggtagtgcat
      781 ggcgtgcgcg cgccgcgcct gttcgaattc gccgctttct tgtaacaggt cgagggcgta
      841 ttcgcgcacc gtcaataaca tctggatata cggctccatg ccttcgatct ggctgcgata
      901 cagcaagctt ttgtccagca gcgacgccag gcgatctatg acgggaacgt tgtcaacttc
      961 gttcatggcc tgctgcatcg cttccaccgc ctctataacc aaagcgccgc gaaagacgga
     1021 gagcctgcgg aacatccgct gctccccggc atccagcagg tcgtagctcc attggatggc
     1081 gttgcgcagc gtctggtggc gcagcggcaa agtgcgcgac tggctggtga gcgtcttgag
     1141 cggctcgtcg agtttcgcga gcagcgcttc aggcgagagg attttcatac gagcagccgc
     1201 cagttcaatc gccagcggca ggccatccag gcgtttgcag atttccgcga tgctttttgc
     1261 gttggtctgc gtcatctgga atgacgcaat cgcccgtgcg cgctgcacaa acagggcaat
     1321 cgccgcgtat tgcgtgagca actcctcttg tgtgagcatc ttcatgtcgc tgggaaacgc
     1381 gagcggcggc acgtgaaaga cctgttcagc atccaggttg agcgtggcgc ggctcgtcac
     1441 cacaaccttc aaacgcgggc aggcgatcag gagatcttca atcaatgggg cagtttctac
     1501 cacctgctca aagttatcga gtaacagcag gaactctttc ttgctcaaag atgccttgat
     1561 cgcttcaaga ataggctgtc cgccgatctc ttgaatgcca agctcctgcg caattcttgg
     1621 tatcaccagg tcggtttcgc tcacagcagc caggtgaacg aagcagatgc cgtcggcgag
     1681 atgcggctgc atctccctgg caatctggaa actcaggcgc gtcttgccga tgccgcccgt
     1741 gccggtcagc gtcagcaggc gcgtggcgcg caagaggcgc ctcacctcgg ccatctcccg
     1801 ctcccgcccg atgaagctcg tccactggac cggcagattg tgcgcgaatg cttccaacga
     1861 gcggagcggc ggaaactccg ccggcaaatc ggggtgcacc aactggaaga gcggctcggc
     1921 gcgctgcaga tccttgagcc gatgcgttcc cagttctcgc aggctggctc ccggcggcag
     1981 gtcatcgcgc gccagggccg ccgtggt
//
LOCUS       DASZAK010000028         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_320085, whole genome shotgun sequence.
ACCESSION   DASZAK010000028 DASZAK010000000
VERSION     DASZAK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320085"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1551
                     /gene="murJ"
                     /locus_tag="VGT44_00460"
     CDS             <1..1551
                     /gene="murJ"
                     /locus_tag="VGT44_00460"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEV2579294.1"
                     /translation="QAFLVPDLIFNIVAGGALSSAFIPVFTEYMIGRHDEKQAWHIAS
                     SALNLAIALMMVLAGLAILFAPVIVPLYNPGLNPKIPADKAELDLIISLSRIMFLQSI
                     LLGGGVILTSILNAKQDFLRPAIGTVLYNVGLIGGLLPCVFIVADRSAAHETTAVFLA
                     TWGVVIGAILQVAAQLPGIVQVKMRYTFSFDYHHPGVIQIGRQMAPRVLNAAMLYFST
                     FVDRGLILLMVAGVSQLATNGYITQYYQAFQLVLLPLGIFGMAISTAAFPTLAENVTL
                     GRMDRVRAIIQDTLRTILFMSIPSSVGLIVLGLPIIQVLLQHGAYDLASAESTSIPLA
                     FFAVGLAGLASVEILTRSFYAMRDSRTPVLISIAQFIFKIALSIIFILVAINWGLSWG
                     LGALALSTSIAGLLEALVLLWLLHQRVGGLALRSLRVFVERVLLASLAMGVAIFVLRL
                     LLDLIFSTYHDRLGFGDTLLAFIKLLLELALGIFVYIRATRMLGIEDFWQQGPVKRVL
                     ERLRLSWI"
     gene            complement(1560..2987)
                     /locus_tag="VGT44_00465"
     CDS             complement(1560..2987)
                     /locus_tag="VGT44_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2579295.1"
                     /translation="MIQRNPRVIGGIYQTGPVITSGPMLTTYTGYNRNNGDVVGLHVI
                     EIPPMVEEAEAMRLLQPLEQRKQALSEHVIHVYDWGMDGSRVFIATDPPRGITLRHVL
                     DNENVEMSRVLNLTRQMARGLIVLQAQSLTGIDMRPQLITIDRLGETDRAQLDDIGLR
                     QLLRQFGYTQGLQPGDIGSLDPRYAAPEQIQQGLIGSWSDVYQLGLLIFEMVTGRLPF
                     VGRTATETGILQVSSPAPRMAQFTQTAPQALQDLLDRVLSKDPSQRLPNAAAMLDALN
                     AIPTLSASQRTPLSSPDLLLTPTYGIPSFPTQPKGQTGEMAKMPLEQGDIALQGTVIN
                     EDSTVFRPGNTPVEATGIIEMEEDVLAYLTLEHEGHESRRFPIKNSYVVVGRLDPKRD
                     IRPEVDLTNVDPRMTVSRQHARIRYEKTFFYIEDLKSHNKTRLGELTLTPLKAELLQH
                     GDVVQFGSVRMVFRVPGMKDLPVLK"
BASE COUNT          534 a          880 c          901 g          738 t
ORIGIN      
        1 caggcgtttc tggtgcctga cctgatcttc aacatcgtgg ccggcggcgc gctcagttct
       61 gccttcatcc cagtattcac ggagtacatg atcggacgcc atgacgaaaa acaagcctgg
      121 cacatcgcca gttccgccct caacctcgcc attgccctta tgatggtgct ggccgggctg
      181 gctatccttt tcgcgccggt gatcgtgcca ttgtataacc ccggcctcaa cccgaaaatc
      241 ccagcggaca aggctgaact tgacctgatt atctcgctgt cgcgtatcat gttcctgcaa
      301 tcgatcctgc tgggcggcgg cgttattcta acttcgatcc tcaacgcgaa acaggatttc
      361 ctgcgacccg ccatcggcac ggtgctgtat aatgtggggc tgatcggagg actgctgccc
      421 tgcgtcttca ttgtggccga ccgctcggcg gcgcacgaga caacggcggt attcctggct
      481 acgtggggtg tggtgatcgg cgcgatattg caggtcgccg cgcagcttcc tggcatcgtc
      541 caggtcaaga tgcgctatac cttttccttc gattaccatc atcctggcgt cattcagatt
      601 ggccgccaga tggcgccgcg cgttctcaat gccgctatgc tgtacttctc gacctttgtg
      661 gatcgcgggc tgattctttt gatggtcgca ggcgtcagcc agcttgccac caatggatac
      721 atcacgcagt actaccaggc gttccagctg gtgctgctgc ccctgggcat ttttggcatg
      781 gccatctcga ctgcggcctt tcccacgctg gccgagaatg tcacgctggg acgcatggac
      841 cgtgtgcgcg ctatcattca ggataccctg cgcactatcc tgttcatgtc catcccttcc
      901 agcgttgggc tgatcgtact gggcctaccc atcatccagg tgctgctcca gcacggcgcg
      961 tatgacctgg cgagcgcgga gagcacctcg atccccctgg catttttcgc cgttggactg
     1021 gctggactgg catccgtcga gattctgacc cgttcgtttt atgccatgcg cgacagccgg
     1081 acgccagtat tgatcagtat cgctcagttc attttcaaaa ttgccctgag tatcatcttc
     1141 atcctggtcg cgattaactg ggggctctcg tggggactgg gcgcgctggc tctctctacc
     1201 tcgatagcgg gcctgctcga ggcattggtg ctgttgtggt tgctgcatca acgggtgggc
     1261 ggcctggcgc tgcgctcgct gcgcgttttt gtagagcggg tgctgctggc gtcgctagcg
     1321 atgggtgtgg cgattttcgt gctgcgcttg ctgctcgacc tgatcttctc aacctatcac
     1381 gaccggctcg gctttgggga taccctgctg gccttcatca aactgctgct ggaacttgcg
     1441 ctcggcatct tcgtttatat ccgcgccacg cgaatgctcg gcatcgaaga tttctggcag
     1501 caggggccgg tgaagcgcgt cctggaacgc ctgcgcctct cgtggattta gcgacgacct
     1561 cacttgagaa ccggcagatc cttcatcccc ggcacgcgaa agaccatgcg cacggaacca
     1621 aactggacga catcgccatg ctgtaacaac tcggccttga ggggggtcaa ggtcagttca
     1681 cccagacgtg ttttgttatg gcttttgagg tcttcgatat agaaaaaggt cttctcgtag
     1741 cggatacggg catgttggcg ggagacggtc atacgcgggt caacgttggt caggtctact
     1801 tcagggcgaa tatctcgctt ggggtccagc ctgcctacca ccacgtagct gtttttgatg
     1861 gggaagcggc gtgattcatg gccttcatgt tcaagcgtga gataggccag gacatcttcc
     1921 tccatctcaa tgatgccggt tgcctcaacc ggagtgttgc ctggtctgaa taccgtcgaa
     1981 tcttcgttga tgaccgttcc ctgcaaggca atatctccct gctcaagcgg cattttggcc
     2041 atttcacccg tctggccttt cggttgtgtc gggaaggagg gtatgccgta ggtgggggtc
     2101 agcagcagat ccggggaact cagcggggtt ctctgggaag cgctgagagt agggatcgca
     2161 ttcaaggcgt cgagcatggc tgccgcgttt ggaaggcgct gggaagggtc tttggacaag
     2221 acgcgatcaa gcaggtcttg caatgcctgc ggggcggttt gcgtgaattg cgccatgcgg
     2281 ggcgcgggac ttgagacctg caagatgccg gtttccgtcg ctgtgcgccc gacaaatggg
     2341 agacgccccg tcaccatctc gaagatcaaa aggccaagct ggtacacgtc gctccaggag
     2401 ccgattagcc cctgctggat ttgctccggc gctgcgtagc gagggtcgag tgagccgatg
     2461 tctccgggct gcaagccctg ggtatagccg aattgccgca atagttgacg caggccgata
     2521 tcatccaact gcgcgcggtc agtttcacct aacctatcga tggtgattaa ttgcgggcgc
     2581 atatcgatac cggtgaggct ctgtgcttgc aggacgatca gcccgcgcgc catttgccgc
     2641 gtgagattta gtacgcgcga catctcaacg ttctcgttgt ccagcacgtg gcgcagggtg
     2701 atgccgcgcg gcggatcggt ggcgatgaag acgcgcgacc catccatgcc ccagtcatac
     2761 acgtgaatga catgctctga cagcgcctgc ttgcgctgct ccaacggctg taacaggcgc
     2821 atggcctccg cttcctccac cattggcggt atttctatga catgcagccc taccacgtcg
     2881 ccgttattgc ggttatagcc ggtataggtc gtgagcatgg ggccggatgt aatcaccggc
     2941 ccggtctgat agatgccgcc gatcacgcga gggttgcgct ggatcatggt cttctccctg
     3001 ttcgcttgcc aggttttctc ttagattacc acaatcttgc ggggctgtca tta
//
LOCUS       DASZAK010000029         2989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_462101, whole genome shotgun sequence.
ACCESSION   DASZAK010000029 DASZAK010000000
VERSION     DASZAK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2989
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462101"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..665)
                     /gene="nadA"
                     /locus_tag="VGT44_00470"
     CDS             complement(<1..665)
                     /gene="nadA"
                     /locus_tag="VGT44_00470"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEV2579296.1"
                     /translation="MALQSACASSKEERVLPLLYPETRARHSHSKRIDTIPQRYAEMS
                     GEELDERIAAARAILGERLVILGHHYQRDEIIKYADLRGDSFKLAQLAAARPQADYIV
                     FCGVHFMAESADILSAPHQKVILPNPAAGCSMADMANIAEVEECWEMLIDTLGNDAGI
                     IPVTYMNSAANLKAFCGRNGGIVCTSSNAPAVIKWAFSRGKRVLFFPDEHLGRNTALK
                     FGIP"
     gene            complement(685..1527)
                     /gene="nadC"
                     /locus_tag="VGT44_00475"
     CDS             complement(685..1527)
                     /gene="nadC"
                     /locus_tag="VGT44_00475"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV2579297.1"
                     /translation="MPNVPLDLIQHALAEDGAEADITTLCTVPVEQQTRANIIARQEG
                     IIAGLAVAAATFRELDPRVSVELLVEDGASVQAGHVVAHISGPARSVLSAERVALNFL
                     GHFSGIATLTAQCVSALEGTKARILDTRKTTPGLRSLEKEAVRMGGGHNHRFNLSDGV
                     LIKDNHIKAAGGIVSAITLARRLAPHLLKIEVECETLLEVEEALGAAADVLLLDNMPV
                     ESMHRAVELVRRVAPKVLIEASGNIGTNPAKLAEVAGTGVDFISLGAITHSASNFDVS
                     LEFA"
     gene            complement(1520..>2989)
                     /locus_tag="VGT44_00480"
     CDS             complement(1520..>2989)
                     /locus_tag="VGT44_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV2579298.1"
                     /translation="LLLGREAAHSRWRVLHAGGDATGAEIERALMTAVRKRSSIVLYE
                     ETYVRSLSVENGVCTGLEAVRRNGQAITIEAGATILANGGAGGLWLHTSNPAGATADG
                     IALAWRAGAALTDLEFMQFHPTVLVVDGGSHLVSEAVRGEGAYLRNHAGERFMPRYHA
                     MGELAPRDVVARSILSEMLSEGTDCQYLDLRHLPAERMHERFPTISAICRGYGLDLAN
                     DLLPIAPAAHYCMGGVMTGTFGRTTLEGLYAVGEVACTGVHGANRLASNSLLEGLVFG
                     LRLADGLAGTIAEQVIPSSRQAITIPVFDDLSREETLLRIELDQENILRMQREIRQVM
                     WQYVSLQRDRQGLLDARHQLHNLRHRVLANGTARQDKQHIAVAWLETVNMLKVAELVI
                     VAALQRRESRGSHWRLDYPTFDDTLAGCHFAFIPQGNSLKQGAHVIGDGSITGDRVTE
                     GDRVLPEGGRKGAPLQYYEGNAGAALCMVGEQEEVIFHA"
BASE COUNT          650 a          868 c          819 g          652 t
ORIGIN      
        1 ggaattccga acttgagcgc ggtgttacgc cccaggtgtt cgtcaggaaa gaagagtacc
       61 cgtttgccac ggctgaaggc ccatttgatc acggccggtg cgttcgagga ggtgcatacg
      121 atgccgccat tgcgcccgca gaaagccttg agattggccg ccgagttcat ataggtaacc
      181 gggatgatac cggcatcatt gcccaatgta tcaatgagca tttcccagca ttcctcaacc
      241 tcggcgatat tcgccatgtc tgccattgag cagcccgcgg cgggattggg caggatcact
      301 ttctggtgag gtgcgctcag gatatcggcg ctctccgcca tgaaatgcac tccacagaag
      361 acgatgtaat cggcctgggg acgcgcggct gccaactgcg cgagcttgaa ggaatcacca
      421 cgcaaatcgg cgtacttgat gatttcgtca cgctggtaat gatgccccag gatgacgaga
      481 cgctccccca gaattgccct ggcggcggcg atacgctcat ctaattcctc gcctgacatt
      541 tctgcatagc gctgcggaat ggtatcaata cgctttgaat gactatgtcg agcgcgtgtc
      601 tcggggtata agagcggcag aacccgctcc tctttgctcg atgcgcacgc tgattgtaga
      661 gccatgtctt ttctccttga aaaatcaagc gaactctaat gatacgtcga aattggaagc
      721 ggaatgggtt atcgctccaa gcgaaatgaa atcaacgccg gtgcccgcaa cctcggccaa
      781 ttttgccgga tttgtgccga tgttgcctga agcctcgatc aatacttttg gagcgacgcg
      841 gcgcactaac tctacggcgc ggtgcatgct ttcaacaggc atgttatcca gcaacagcac
      901 atctgctgct gctcccagcg cctcctccac ttccaggagt gtttcacact ccacctcgat
      961 tttgagcagg tggggagcaa ggcgacgggc tagagtgatt gcagacacaa tacctccagc
     1021 tgctttgata tggttgtcct taatgagcac cccgtcactc aggttgaagc ggtggttgtg
     1081 acctccaccc atgcgcaccg cctctttttc caggctgcgc agaccgggcg tggttttgcg
     1141 tgtatcgagt atacgtgcct ttgtcccctc gagcgcgctg acacactggg ctgtaagtgt
     1201 agcgatgcct gagaagtgtc ccaggaaatt caaggcgaca cgttccgcgc ttaacacaga
     1261 gcgagcgggg ccgctgatat gcgcaaccac atggcctgcc tggacgcttg cgccatcttc
     1321 taccagcagt tcaaccgata cccggggatc cagttcgcga aatgttgcgg cggcaacggc
     1381 cagaccagcg attatcccct cctgccgggc aataatgttc gcccgggttt gttgttctac
     1441 gggaacggtg caaagtgtcg tgatatcagc ctccgctccg tcctctgcaa gagcatgctg
     1501 gatgagatcg agtggcacgt taggcatgga atatcacctc ctcttgctcc ccaaccatac
     1561 acagagctgc tccggcattc ccttcgtaat attgtagggg cgcgcccttg cggccgccct
     1621 cggggaggac ccgatcaccc tccgtaacac gatcacccgt gatactgcca tccccaatca
     1681 catgggcacc ctgtttgaga ctattgcctt gaggaatgaa ggcaaagtga cagccagcca
     1741 gggtgtcatc aaaggtggga tagtcgagtc tccagtggct tccgcgactc tcacggcgtt
     1801 gcaaggcagc gacaattacg agttcggcga ctttgagcat gttgacggtc tccagccatg
     1861 ctactgcaat atgttgtttg tcctgtctcg ctgttccgtt tgccagcacc cggtgacgaa
     1921 ggttatgaag ttgatgcctg gcatcgagaa gcccctgacg atcgcgctgg agtgagacat
     1981 attgccacat gacctggcgt atttcgcgct gcatgcgcag gatgttctct tgatcaagtt
     2041 cgatgcgcag cagagtctct tctcgtgata ggtcatcaaa aacgggtatc gtaatggctt
     2101 gccttgatga ggggataacc tgctcagcaa tcgtccccgc aagtccatcg gccaggcgca
     2161 gcccaaagac cagtccctcc aataacgagt tgctggccag cctgtttgct ccgtgtactc
     2221 ctgtgcaggc aacctctcca actgcgtaga gaccctcaag cgtcgtgcga ccaaaggtac
     2281 cggtcatcac ccctcccatg caatagtggg ccgccggggc gataggaagc aggtcgtttg
     2341 ccagatccag accatacccg cgacagatag cggatatggt ggggaaacgt tcatgcatac
     2401 gctcggcagg aagatgccgc aggtcaagat actggcaatc ggtaccttcg gacaacatct
     2461 cactaagaat ggaacgagca acgacatcac gaggagcaag ttctcccata gcatgatacc
     2521 ggggcatgaa gcgctctcct gcatggttgc gcagataggc tccttcgccg cgcaccgcct
     2581 cggatacaag gtgagaaccg ccgtcgacta ccagcacggt cgggtggaac tgcataaatt
     2641 cgagatccgt aagggccgct ccagcacgcc atgccagggc gataccatca gcggttgctc
     2701 cggcagggtt tgaggtatgt agccagagac cgccagcgcc accgttagct aaaatcgttg
     2761 cccctgcctc gatggtgatg gcctgaccat ttcgacgaac ggcttcaaga ccggtgcaaa
     2821 cgccgttctc aacgctcaga ctgcgtacat atgtctcctc gtacaatact attgaggaac
     2881 gtttgcgcac ggctgtcatg agcgctcgtt ctatctctgc accggtggcg tcaccgcccg
     2941 cgtgcaagac gcgccagcga ctatgcgccg cctcacgtcc aagcagcaa
//
LOCUS       DASZAK010000030         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_568522, whole genome shotgun sequence.
ACCESSION   DASZAK010000030 DASZAK010000000
VERSION     DASZAK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568522"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VGT44_00485"
     CDS             complement(<1..1161)
                     /locus_tag="VGT44_00485"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEV2579299.1"
                     /translation="MSNPFQRLRNIIFGPQHENGTYPGREGPYGATGGEDARPVFTAL
                     DIGTAYAKALIIEVQDDIGVVLGVGRHTQSYSHMSDGIVTDIPGVIANCNEALIQAEQ
                     SAGGVIAPSAVIGIAGELVKGSSITITKQRPQPTKPIAPEELEGMISSAQQKLLKTAK
                     ERIAAETGYQNIEVRLTNAAVISVRIDGQTVINPIGFRGRHFTLTLFSAFAPLMQLGA
                     LETVAQGLDLTLVTIVAEPYALARCLSTNASANSGAIFIDIGGGTTDIALVRQGGIEE
                     TRMFALGGRTFTRRLAASKGLPLKDAEKLKLNYGSGGVKGGEREEIQKIFASESQTWM
                     DSVELLVDELAKGELLPPAIYMVGGGSALPDLREKLENFPWTERLPFSREPII"
     gene            complement(1297..>2808)
                     /locus_tag="VGT44_00490"
     CDS             complement(1297..>2808)
                     /locus_tag="VGT44_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEV2579300.1"
                     /translation="EELKQRGHTAVPGDEAFKLYDTHGFPLELTQEVAAEQGFTVDVS
                     GFEQSMQRQQERSRAASTFAQGRDDEALAEVLKRVGPTEFTGYEGVTGSGRVAGLIVE
                     GVEVDSISAPQEALVVLDKTPFYAESGGQIGDRGDLSGSMGVFQVQDTRRPIKGLIVH
                     YGRISEGYIRVGDSVQANVVAQRREDTMRNHSATHLLHKALRDIIGPQVEQRGSLVEP
                     ERLRFDFACPRALTATEIALVDEQINRWIRGDRPVHTNIMPLQEALMTGAMALFGEKY
                     DEEVRVVSMGSSVELCGGTHCAATGQIGLYITIQETSIAAGVRRIEALTGRGALAYLR
                     QRSALVDALSARLQTQPDMLEARVDQVAQELAAARRQVAQFQREAAQRQAEALAQKAQ
                     YAAGVPVVATTADVPDERVLREMGDMVLNKLGRPGVVVLAGNFEDRVGIQVSVSSELA
                     KRGIHAGKIAGAVGQRLGGKGGGRPESAQGGGKNKAELGAALDLVPKLVGEAL"
BASE COUNT          543 a          909 c          732 g          624 t
ORIGIN      
        1 gatgatgggt tcccgcgaaa acgggagtcg ttctgtccag gggaaatttt cgagtttctc
       61 gcgtagatcg ggcagcgcgg aacccccacc caccatatag atcgccggtg gtagaagctc
      121 gccctttgcc agttcgtcaa ccagcaactc tacactgtcc atccatgttt gagattccga
      181 ggcgaaaatt ttttgtatct cctcacgttc gccccctttc accccgccgc tgccgtagtt
      241 caatttgagt ttctcggcgt ctttgagcgg aaggcctttg ctggccgcga gacggcgtgt
      301 gaacgtacgc ccacccagcg caaacatgcg cgtctcttcg atcccgccct ggcgcaccag
      361 cgcgatatcg gtcgtgccgc cgccgatatc aatgaagata gcgccgctat ttgcgctcgc
      421 attcgtactc aggcagcgag ccagcgcata tggctcggca acaattgtga ccagcgtcaa
      481 atccaggccc tgcgccaccg tctccagcgc gcctaactgc atgagcgggg caaaggcgct
      541 aaacaacgtt agcgtgaagt gccgcccgcg aaagcctatt ggattgatga ccgtctgccc
      601 atcaatacgc accgagatca ccgccgcgtt ggtcaggcgt acctcaatat tctgataacc
      661 ggtctcagcg gcaatgcgct ccttggcggt tttcagtaat ttctgctgcg cgctggagat
      721 catgccctcc aattcctccg gcgcaatggg tttggttggc tgcggccgtt gtttggtgat
      781 ggtgatcgag ctgcccttga caagctctcc cgctatacca atcactgctg aaggagcaat
      841 gactccgccc gccgattgct ccgcctggat cagggcttcg ttgcaattag caatgacgcc
      901 tggaatatcg gtaacgatgc catcagacat gtggctatag ctttgtgtgt ggcgtcccac
      961 tcccaacacg accccgatat catcttgtac ttcaatgatc aaggccttgg catatgccgt
     1021 gccaatgtcg agcgcggtaa agacgggacg tgcatcctcc ccgccagtag cgccatacgg
     1081 cccctcacgg cccggatagg tgccattctc gtgttgtggt ccgaatataa tattgcgtaa
     1141 acgctgaaag ggattactca tgttttcctg gactgtgttg attatgacca tactaaagaa
     1201 gagaacgtac agcttaccga actcgttggt ccagtctcaa ctcttgctta ttgagtacgt
     1261 tatagcacgt acgtcgagct actgcaaggg ttatcttcac aaggcctcac ccaccagctt
     1321 tggcaccaga tcgagggctg cacctaactc agctttgttt ttgcctccac cctgtgcaga
     1381 ttccggcctg cccccgccct tgccacccag gcgctggcct accgctcccg caattttacc
     1441 ggcgtggata ccgcgcttcg ccagctcgga tgaaacgctc acctgaatcc ccacgcggtc
     1501 ctcgaagttg ccggcgagta cgaccactcc tggccggccc agcttgttga gcaccatatc
     1561 acccatctcg cgcagcactc tctcatcggg cacatcggct gttgtcgcta cgaccggcac
     1621 accggctgca tattgggcct tttgagcaag cgcttcggcc tgtctctggg cggcttcgcg
     1681 ttggaattgt gcgacctggc ggcgtgcagc ggcgagttcc tgcgccacct ggtcaacccg
     1741 cgcctcgagc atatccggct gtgtctgcaa cctggcgctg agcgcatcta ccagggcgct
     1801 gcgctggcgc aggtaggcca gagcgccacg acccgttaaa gcctcgatac gacgaacgcc
     1861 ggcagctata ctcgtttcct ggatcgtgat atacaacccg atctgacccg tggccgcaca
     1921 atgcgtaccg ccgcacaact caacactgct gcccatcgag accacccgta cttcctcatc
     1981 gtacttttcg ccaaaaagcg ccattgctcc cgtcatcagc gcttcttgca gtggcatgat
     2041 attcgtgtga accggacggt cgccgcggat ccagcggtta atctgctcgt caaccagcgc
     2101 tatctcggtg gcagtgagcg cgcggggaca ggcaaagtcg aagcgcaaac gctccggttc
     2161 gaccaatgag ccgcgctgct ccacctgggg accgatgatg tcgcgcaagg ctttatgcag
     2221 caggtgggtg gcgctgtggt tgcgcatggt atcttctcgc cgctgagcaa ccacgtttgc
     2281 ctgcacgctg tcacccacgc gtatgtagcc ctcgctaata cgcccatagt gaacaatcag
     2341 cccttttatt ggacggcgtg tatcctgcac ctggaaaacg cccatcgaac cgctgagatc
     2401 acctcgatca ccaatctggc ctccgctctc tgcatagaat ggagtcttat cgagcaccac
     2461 cagggcctcc tgcggggcgc tgatactatc aacctcgacc ccctctacaa tcaaaccggc
     2521 caccctgccg ctgccggtga ctccctcgta gcccgtgaac tcggtcggcc caacacgctt
     2581 gagcacttcg gcgagcgctt cgtcatcgcg cccctgtgcg aatgtgcttg ctgcccggct
     2641 gcgctcctgc tggcgctgca tggattgctc gaaccccgag acatctacgg tgaagccctg
     2701 ctccgccgcc acctcctgcg ttagttccag cggaaaacca tgtgtatcgt acagcttaaa
     2761 agcctcgtca cccggtacag cagtatggcc gcgctgcttc agctcctc
//
LOCUS       DASZAK010000031         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_107199, whole genome shotgun sequence.
ACCESSION   DASZAK010000031 DASZAK010000000
VERSION     DASZAK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107199"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1568)
                     /locus_tag="VGT44_00495"
     CDS             complement(102..1568)
                     /locus_tag="VGT44_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2579301.1"
                     /translation="MSTSYKYDAVVIGAGPNGLAAAITLARAGHSVIVYEAKATIGGG
                     SRSMELTLPGFVHDVCSAIHPLGLASSFFRTLPLEQYGLDWIHPLAPLAHPLDDEPAV
                     MLERSLEETAAALGRDAAAYRELMEPLAAHWEIIADAFLGPLRLSPLVRHPFTLGQFG
                     LTALRTARGLAEHIFREPRARALFAGICAHWMLRLEQMPSAAPGMVMGVLGHSVGWPM
                     PKGGSQHIVDALAAHLRELGGDIVTGVEIKSLEMLPTSRVVLCDVTPRQLLRIAGSRL
                     PVGYQRCLQRFRYGPGVFKLDLALDGPIPWKATECSRAGTVHLGGTLPEMAAAEAAVW
                     RGEHPDHPFVLLAQPSLFDPSRAPAGKHTAWAYCHVPNGSTFDMTARIEAQIERFAPG
                     FRDRILARHTFSAAQMQQYNANYIGGDINGGVQDLWQLFTRPTIQVNPYATPLKGVYL
                     CSSSTPPGGGVHGLCGYFAAQAALKEQLHVSMGHRDLR"
     gene            complement(1599..2378)
                     /locus_tag="VGT44_00500"
     CDS             complement(1599..2378)
                     /locus_tag="VGT44_00500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579302.1"
                     /translation="MTNAPLPADGQAQQVGQSIQASEQPRDAPYWSHSASELKVSNVP
                     SGALNLNVDGKLAVGPLQGFGKMWQKTYRIALKGATVEAKEVILTWKKNFPRFWPAGN
                     RFYAPLTGIAPGEVALLSIKAGGVALSTGVLVLYADEESFTLMTPQGHVFAGWITFSA
                     YAESDVVVVQAQVLMRANDPIYELGLRFGGHKQEDLFWQHTLKSLAAEFGVSAQVQTE
                     VVCIDPRLQWSQAKNIWHNAAMRTTIYRVTTPLRWIRGKRT"
     gene            complement(2454..2804)
                     /locus_tag="VGT44_00505"
     CDS             complement(2454..2804)
                     /locus_tag="VGT44_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV2579303.1"
                     /translation="MPQANVAMHVRQVNQATSVIDIRGDVSAFAEQVLMDAYNEANTP
                     TTRAIILNFAGLEYMNSSGIGLIVTLLIRVNRQKQRLLACGLSDHYKHIFELTRLSDA
                     IHIFESETEAIHEM"
     gene            complement(2882..>3225)
                     /locus_tag="VGT44_00510"
     CDS             complement(2882..>3225)
                     /locus_tag="VGT44_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV2579304.1"
                     /translation="VRHQPRVAIIDLHGEINAFAEDVLNTAYTDAESRELDVILLNFS
                     DVDYINSTGIALIVSLLARARKTKRRLLACGLSQHYVEIFQITRLVDFMSVFPDETSA
                     LKENPTPIIQN"
BASE COUNT          662 a          945 c          969 g          649 t
ORIGIN      
        1 tgcccgaagc cctggccgcc attctcgcca tcgtgcgagc gcagcagctc gcgttctcgc
       61 tcgccggtgt gcatggccgc aatcccgacg cgccatctgg gctatcgaag gtcacgatga
      121 cccattgaaa cgtgtagctg ctcttttaag gcagcctggg ccgcgaaata gccgcacagc
      181 ccatgcacgc cgccgccggg aggcgtagac gaggaacaga ggtagacgcc cttgaggggt
      241 gtggcgtagg gattgacctg gatggtgggt cgggtgaata gttgccagag atcctgcacg
      301 ccgccgttga tgtcaccgcc gatatagttg gcgttgtact gctgcatctg cgccgccgaa
      361 aaagtgtgcc gcgccaggat gcggtcgcga aaaccggggg cgaagcgttc gatctgcgct
      421 tcgatgcgcg cggtcatgtc gaaggtcgaa ccgtttggca cgtgacagta ggcccaggcg
      481 gtgtgcttgc cagcaggcgc gcgcgatgga tcgaacaggc tgggctgggc cagcagcacg
      541 aagggatggt cgggatgctc gccccgccag acggcagcct cagcagccgc catctctggc
      601 agggtaccac cgagatgcac cgtcccggcg cggctgcatt ccgtcgcttt ccagggaatg
      661 ggaccatcca gcgccaggtc gagcttgaag acgccaggac catagcgaaa gcgttgcagg
      721 cagcgctggt agccgacagg caggcgcgac ccggcgattc gcagcagttg ccggggcgtc
      781 acgtcgcaca gcacaacacg cgaggtcggc agcatctcca gcgacttgat ctccacaccg
      841 gtcacgatgt cgccacccag ctcgcgcaga tgagccgcca gcgcgtcgac aatgtgctgc
      901 gagccgccct ttggcatcgg ccagccaaca ctatgcccca gtacgcccat taccatcccc
      961 ggcgcagcgc tgggcatttg ctctaaacgc agcatccaat gcgcgcagat gccagcgaag
     1021 agggcgcgcg cgcgcggttc gcggaagatg tgttccgcca ggccacgcgc ggtacgtaga
     1081 gcagtcaagc caaattgacc cagcgtgaaa gggtgcctga ccagtggaga gaggcgcagc
     1141 gggccaagaa aggcgtcggc gatgatctcc caatgtgcag caagcggctc catcagctcg
     1201 cgatacgcgg cagcatctcg cccaagtgcg gcggctgtct cttctagcga gcgctcaagc
     1261 atgaccgccg gttcatcatc gagcgggtgc gcgagcggcg cgagcgggtg aatccagtcc
     1321 aggccatatt gctcgagggg caacgtgcgg aaaaaggacg aggccagccc aagcgggtga
     1381 atcgccgaac atacatcatg cacgaaaccg ggcagcgtca gctccatcga gcgcgaaccg
     1441 ccgccgatgg tcgctttcgc ctcgtagacg atcaccgaat gacccgcgcg cgccagcgtg
     1501 atcgcggctg ccaggccgtt cggccctgcc ccgatgacga cggcgtcata cttgtacgac
     1561 gttgacatgg ctatcttgct tcttcgtgag gaaacaactt atgtcctttt tccgcgtatc
     1621 caacgcaagg gagttgtcac cctgtatatc gtagtgcgca tcgcggcatt atgccagatg
     1681 ttcttggcct gcgaccactg caggcgcggg tcgatgcaga ccacttccgt ttgcacctgt
     1741 gcgctgacgc caaactcggc ggccagcgat ttcagcgtat gttgccagaa gagatcttcc
     1801 tgcttgtgtc ccccgaaacg caagccgagt tcgtaaatgg ggtcattggc gcgcatcaac
     1861 acctgagcct ggacgacaac aacgtcactc tccgcatagg cgctaaaggt aatccagccc
     1921 gcgaagacgt gaccctgcgg cgtcatcaag gtgaaggact cctcatcggc gtagaggacc
     1981 agcacgccgg tggaaagcgc gacgccaccg gccttgatac tgagcagggc aacttcgccg
     2041 ggggcaatac ccgtcaatgg ggcatagaag cggtttccgg caggccagaa gcgcggaaag
     2101 tttttcttcc aggtcaggat gacctccttc gcctccaccg tcgcgccttt cagcgcaata
     2161 cgataggtct tctgccacat ctttccgaac ccttgcagcg gcccgacagc cagtttccca
     2221 tcgacattca gattgagcgc gccgcttggc acatttgata ctttcagttc ggaggcggaa
     2281 tgcgaccagt agggagcgtc gcgtggttgc tcgctcgctt gaatggattg cccgacctgc
     2341 tgcgcctgtc catcggctgg aagtggtgca tttgtcatag ttagcttcct ctttgttctt
     2401 gttgactgca aaggtaacgc ccctgattaa ccaggacaac cctgagcgcg gcgttacatt
     2461 tcgtgtatag cttccgtttc gctttcgaag atatgaatgg catcgctcag gcgtgtcaat
     2521 tcaaagatgt gcttgtaatg gtcgctcagt ccgcaggcga gcaggcgctg tttctggcgg
     2581 ttgacacgaa tgagcagggt gacgatcagg ccgatcccgc tactgttcat atactctaat
     2641 cccgcgaagt tcaggatgat agcgcgcgtc gtaggcgtgt tggcctcgtt ataggcatcc
     2701 atcagcacct gttccgcgaa ggcgctgaca tcgccacgga tatcgatgac gctggtcgcc
     2761 tggtttacct ggcgaacatg catggctaca ttggcctggg gcataaagaa cctcctctgt
     2821 tgaatcgttc aaatgaacat ggtatttgta ctgtcggtgt gataggtata gatattcggt
     2881 tttaattttg aatgatgggc gtagggtttt ccttcagtgc gctggtttca tcgggaaaga
     2941 cgctcatgaa atcgaccagg cgcgtgatct gaaaaatctc cacgtagtgc tggctgagac
     3001 cacaggccag cagccgccgc tttgttttgc gggcgcgcgc taacaagctg acgatcagag
     3061 cgataccagt actgttgata taatccacat cgctgaaatt gagcaggatc acatctaact
     3121 cccggctctc tgcatctgta taagcggtgt tcagcacatc ctctgcaaat gcgttgattt
     3181 ccccgtgcaa atcgataata gcgacgcgcg gctggtggcg aacaa
//
LOCUS       DASZAK010000032         5463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_462241, whole genome shotgun sequence.
ACCESSION   DASZAK010000032 DASZAK010000000
VERSION     DASZAK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462241"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(11..1246)
                     /locus_tag="VGT44_00515"
     CDS             complement(11..1246)
                     /locus_tag="VGT44_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2579305.1"
                     /translation="MQEQEATQTAQVEETACCIAGAGPAGAMLALLLARKGVQVMLLE
                     EHVDFDRDFRGDTIHPSVMAILDEIGLADRLLELEHTRVETLPVETAGGTFTLADFRP
                     LKTRFNYIMLIPQARFLAFITEEALRYENFKLVMGARVEKLIEEDGYVHGVQYRGVDG
                     WHTVRATLTVGADGRFSRMRKLVGFEPVKTSPPMDVLWFRLPRDSGESELSGGRLAGG
                     HILIQLNRGDYWQVGYIIPKGGYQQLHQAGLEQLRQEIAHLMPDMANRVGTIQEWKQV
                     AVLSVESSRLRRWYRPGLLLIGDAAHVMSPVGGVGINYAIQDAVEAANVLADDLRAGK
                     ASVRALAEVQRRREWPTRIIQGFQNIIQKQVLAKVVASDQPLRVSPTFLFLLRLPVIR
                     ALPAKFIGIGPWAAHVRSR"
     gene            complement(1258..2421)
                     /locus_tag="VGT44_00520"
     CDS             complement(1258..2421)
                     /locus_tag="VGT44_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579306.1"
                     /translation="MNIARILALAARINRQVVRDRRTLALIFLVPLLVMTLLYLVLTN
                     TSSVRTLALVRPTGTGSAQINTLIDNLLPGKDKLHTIDIGASQVNSTLENGNADAAVV
                     FPPDFAQQVFAEQNPTVQIVLEGSDPTVASAMSDLTQTFTHQLGVALAAIKSQAGQPG
                     QPGILPTLSGSLPFATPAPQYLHGGSQYTFDDSIAPVFIGIFSFFFVFLLTSIAFLRE
                     RSQGTIERVMVSPLKRPELVVGYICGFSLFALVQSLIILLFVVFALQVHYSGNLALVF
                     LVTALLTIGSVNLGIFLSTFAQNEFQVVQFVPLVLGVQIFLSGIFWPVKQLPAALQPI
                     SYIMPLTYANDALRGVMLKGEDFTGIFPQLTALLIFALVMVLLSSLTTRRQIA"
     gene            complement(2418..3215)
                     /locus_tag="VGT44_00525"
     CDS             complement(2418..3215)
                     /locus_tag="VGT44_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2579307.1"
                     /translation="MAETKPDSGPIENERGANGERAGEPVVVIDGLVKQFGRLRAVDN
                     VSMTIRAGETCGLIGPNGSGKTTLIRMMVGLVRPTSGTIMVMNERMPSGKIAPSLGYM
                     TQLTALYNDLTAIENMRFFCNIYGLRGKEQQRRIDEMLERVELADRAYTVVSKFSGGM
                     KQRLSLACALVHGPRLAVLDEPTVGVDPELRRSFWDYFAQLNKEGVTIIVSTHHLDEA
                     ARCTRLGLMRLGALLAEDTPLELLRQSGKDNMEDAFLYYAQRQEVTA"
     gene            complement(3266..3874)
                     /locus_tag="VGT44_00530"
     CDS             complement(3266..3874)
                     /locus_tag="VGT44_00530"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2579308.1"
                     /translation="MARTPKIVEDRREQIIDAAMRVFAQKGFTKATNKDIAREAGITP
                     GLIYYYFESKEAVLNAIIEMRTPVQLLSALPPEALALPVGVFLKMLAQRVLEIVESEN
                     FVGLIRVMLPEVIHNPAMAPIPLAGFQRVFAFLTGYFEAKVAAGELRPVDAAMIAQVL
                     FGSMIGFVLRRQILRDPAALEFTHEQIADAIVDTMLNGTAAR"
     gene            4240..>5463
                     /locus_tag="VGT44_00535"
     CDS             4240..>5463
                     /locus_tag="VGT44_00535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2579309.1"
                     /translation="MIVAHLIPGQLLENRYRIVGQIGEGGFGSVYKAEDTKDNNRQVA
                     VKVISLGGLSAEQTIEATDAFNREVTALSDLHHASIPRLYEHFTDPDHWYLVMDFIVG
                     ETLEEHRLRAPDGCLPLEQVVRIGVQLCEVLNYLHTRQPPLIFRDVKPGNIMVTPGGR
                     LYLIDFGVARSFQPGKARDTIAFGSPGFAPPEQYGKAQTTPQSDIYSLGITFYQLLTG
                     IDPSLSPFRFPALRILNTSVPSDLEDLVMQMLELEMCKRPASAADVGQVLQRLLEQQK
                     VAPQASSKTSPAPQAFSFSTQGIPSYIHRGHRQPVLAVAWSPDGRFLASAGEDRTVQI
                     WRAFSGRDLFRYHNHADVVKSIAWSPDGKRIASAGEDHTLHVWDTVLHPLWLRAIVLR
                     LGLGYYFYHNGHSRAI"
BASE COUNT         1153 a         1660 c         1527 g         1123 t
ORIGIN      
        1 tcctgccgcg ctacctggac cggacgtgcg cagcccaggg tccaatgcct atgaacttcg
       61 cgggcagagc gcgtatcacc ggcaggcgca gcaagaacag gaacgtcggc gaaaccctta
      121 gaggttgatc gctggccacc accttcgcga gaacctgttt ttgaatgatg ttctggaagc
      181 cctggatgat gcgcgtcggc cattcgcgcc ggcgctgcac ctccgccagg gcgcgaacgc
      241 tggccttccc ggctcgcagg tcgtcggcaa gcacgttcgc cgcctcgacc gcgtcctgga
      301 tggcgtagtt gatgccgacg ccgccgacgg gcgacatgac atgcgccgcg tcgccgatca
      361 gcagcagccc tgggcgatac cacctgcgca ggcggcttga ctcaacggag agaacagcca
      421 cctgcttcca ttcctggata gttccaacgc gattcgccat gtcgggcatc agatgtgcga
      481 tctcctgccg cagctgctcc aggccggcct ggtgtagttg ctgatagccg ccctttggaa
      541 taatgtagcc aacctgccag taatccccgc gattgagctg tatcaggata tgcccgccgg
      601 cgagccgacc gccggacagc tccgactcgc ctgaatcgcg gggcaggcga aaccacagca
      661 catccatcgg cggcgaggtc ttgaccggct cgaaaccgac cagcttgcgc attcgcgaga
      721 aacggccatc cgctccaacc gtcagcgtcg cgcgcaccgt atgccagcca tccacgccgc
      781 gatactgcac gccatgtaca tagccatcct cctcgatcag cttctccact cgcgcgccca
      841 tcaccagctt gaagttctca tagcgcagcg cctcctcggt gatgaacgcc aggaaacgcg
      901 cctggggaat caacatgata tagttaaagc gcgtcttcaa aggccggaaa tctgccagcg
      961 tgaatgtgcc accagccgtt tccacgggca gcgtctcaac cctggtgtgt tcaagttcaa
     1021 gcagtcgatc cgccaggcca atctcgtcca gaatcgccat gacggatgga tgaatggtgt
     1081 cgccgcgaaa gtcgcgatca aagtccacat gttcttcgag cagcataacc tgcacgccct
     1141 tgcgcgccag cagcagtgcc agcatcgcac cggcaggccc ggcccccgcg atacaacagg
     1201 cggtttcttc gacctgcgct gtttgtgtgg cttcctgttc ttgcatgata tacctcctta
     1261 tgcaatctgc cgccgcgtcg ttagcgaact cagcagcacc atcaccaggg cgaagatcag
     1321 cagagcagtt agctgcggga agatgcctgt gaaatcctcg cctttgagca tgacgccccg
     1381 cagggcatcg ttggcgtagg tcaggggcat gatgtacgaa atgggctgca gtgcggcagg
     1441 caactgcttg accggccaga aaatgcccga caggaagatt tgcacgccca gcacaagcgg
     1501 cacgaattgc acaacctgga attcgttctg cgcgaaggtc gagagaaaga tgcccaggtt
     1561 gacgctgccg atggtcagca gcgccgtgac caggaagacc agcgccaggt tgccgctgta
     1621 atgcacctgc aaggcaaaaa caacaaagag caggatgatg agcgattgta ccagcgcaaa
     1681 aaggctgaaa ccgcagatgt aaccgacgac cagctcaggc cgcttcaacg gcgacaccat
     1741 cacccgttcg atagtcccct gcgaccgctc gcgcaggaaa gcaatcgagg tcaggaggaa
     1801 gacaaagaag aaggagaaaa tgccgatgaa gaccggcgca atcgagtcgt cgaaggtata
     1861 ctgcgagccg ccgtgcaggt attgcggggc aggggtggca aacggcagcg agccgctcag
     1921 cgtgggcaga atcccaggct gccctggttg tcctgcctgg ctcttgatgg ccgccaatgc
     1981 gacgcccagt tgatgcgtaa acgtctgcgt cagatcgctc atggcgctgg caacggtggg
     2041 atcagaacct tccagcacaa tctgcacggt cggattctgc tcggcgaaaa cctgctgcgc
     2101 gaaatccggc gggaagacga ccgccgcgtc ggcattgccg ttctccagcg tgctgttcac
     2161 ctggctcgcg ccgatgtcga tggtatgcag cttatccttg cccggaagca ggttatcaat
     2221 cagcgtgttg atctgcgcgc tgcccgtccc ggtcgggcgc accagcgcga gcgtccgcac
     2281 gctgctggta ttcgtcagca ccaggtaaag cagcgtcatc accagcagcg ggacaaggaa
     2341 gatcagggcc agcgtgcgcc tgtcgcgcac aacctgtcgg ttgatgcgcg cggccagcgc
     2401 cagaatgcgt gcgatgttca tgccgtcacc tcctgccgtt gggcatagta tagaaaggcg
     2461 tcctccatgt tatccttgcc cgattgccgc agcagttcga gtggagtatc ctcggccagc
     2521 agcgcgccca aacgcatcaa acccagcctg gtgcagcgcg ccgcctcgtc caggtgatgg
     2581 gtcgagacga tgatggtcac accctcttta ttcaactggg cgaagtagtc ccagaacgag
     2641 cggcgcagct ccgggtcaac gcccaccgtc ggctcatcca gcacggccag ccgcggcccg
     2701 tggaccagcg cacaggcgag cgagagccgc tgcttcatgc cgccgctaaa tttgctcact
     2761 acagtatagg cgcgatccgc cagttcgaca cgttccagca tttcatcaat gcgccgctgc
     2821 tgttccttcc cgcgcaggcc gtagatgtta cagaagaagc gcatgttttc tatggccgtc
     2881 agatcgttgt agagcgcggt cagctgcgtc atgtagccca gggagggcgc aatcttccca
     2941 ctcggcatgc gctcgttcat caccatgatg gtcccgctgg ttggccgcac cagcccgacc
     3001 atcatgcgga tcagcgtcgt cttgcccgac ccgttcggtc cgatcaagcc gcaggtctcg
     3061 cccgcgcgaa tcgtcatgga cacgttatcc accgcgcgca gcctgccaaa ctgcttgacc
     3121 agcccgtcga taactacaac cggctcgcct gccctttccc cgttcgcgcc ccgctcattc
     3181 tcaatggggc cagaatctgg ttttgtttcc gccatagcat tctccatttt aactaatcag
     3241 ccaattaaca atgtcgcaaa aaaatttatc gtgccgctgt gccattcagc attgtatcga
     3301 cgatggcatc cgcgatttgt tcgtgtgtaa actccagagc cgcaggatcg cggagaatct
     3361 ggcggcgcaa gacgaaacct atcatgcttc cgaagagaac ctgcgcgatc atcgcagcgt
     3421 caaccggtcg caactccccc gcggccacct ttgcttcaaa atagccggtg agaaaagcga
     3481 agacgcgctg aaaccccgcc agcggtatag gcgccatcgc gggattatgg ataacctccg
     3541 gtaacatcac ccgtataaga ccaacgaagt tttcgctctc gacaatctcc aatacacgct
     3601 gcgccagcat cttcaaaaac actcctaccg gcaatgcgag tgcctccggc ggtagagccg
     3661 acagcaactg aacgggagtg cgcatctcaa tgattgcatt caataccgct tccttgctct
     3721 cgaagtagta gtagatcagc cctggcgtaa tgccagcctc gcgcgcgata tccttgttgg
     3781 tggccttcgt aaatcctttc tgcgcaaaca cgcgcatagc ggcatcaata atctgttcgc
     3841 gtcgatcttc aacgattttg ggcgtccgcg ccatctttac agtactcgct tccagggttg
     3901 gtatgcgtca gttcgatatt tgactgactg attaattaaa ttataacgaa gtaagtaaga
     3961 tttgtcaagc tcaaaacgtc gctagcagag aatagacatt ctgtgagggg acttgaatgg
     4021 caccctgact gtcatcctga gcgcagcgaa ggaactcatc gcccggtcgc gctagatcct
     4081 tcgccgcgct caggatgaca ggcgctttct ccaaatgtct gcgtcgctcc tctaagggct
     4141 aattagggtg gcaactggcg tatttacata tgaggctggc aaaatgcatt ggcgcagtga
     4201 tgatgtaagc cttctcttgc cggccaggag tgcttcagta tgattgttgc ccacctgatc
     4261 cctggtcaac tgctcgaaaa tcgttatcgt atcgtcggcc agattggcga aggcggcttt
     4321 ggctccgtct acaaagcaga agatacgaaa gacaataacc ggcaggtggc cgtgaaagtg
     4381 attagcctgg gtggtctttc cgcggagcag accatcgaag cgaccgacgc cttcaatcgt
     4441 gaggtgacgg cgctctccga tcttcaccat gccagcattc cacgtcttta cgaacatttt
     4501 accgatccag accactggta ccttgtcatg gatttcattg tgggggagac gcttgaggag
     4561 catcggctca gagcgccgga tggctgtttg ccacttgaac aggtcgtgcg gatcggtgtg
     4621 cagctctgcg aggttctcaa ctatttgcat acgcgccagc cgccgctcat ctttcgcgat
     4681 gtcaagccgg gcaatatcat ggtcacgccg ggtgggcggc tctacctgat cgatttcggc
     4741 gtggcgcgct catttcaacc tgggaaggcg agagacacta tcgcttttgg ctcgcccggt
     4801 tttgctccgc ctgagcagta tggcaaggcg cagactaccc cccaatccga catctacagc
     4861 ctgggcatca cgttctacca gttgttgacg ggtattgatc catcgctcag cccatttcgt
     4921 tttccggcgc tgcgcatttt gaatacatcg gttcccagtg atctggagga cctggtgatg
     4981 cagatgttgg aactggagat gtgcaaacgc ccggccagcg ctgcggacgt tgggcaggtc
     5041 ctccaacgtt tgctcgagca gcaaaaggtt gcccctcaag cctcttcaaa aacttcgcct
     5101 gcgccacaag ccttctcctt ctcaacgcag ggtattccga gctatattca tcgcggccat
     5161 cgacaaccgg tgctggcagt ggcctggtcg cccgatgggc gctttctcgc ttcggctggc
     5221 gaggaccgca cggtgcagat ctggagggcc ttcagtggga gagacctgtt tcgctatcac
     5281 aaccacgccg atgtggtgaa gagcatcgcc tggtcgcctg atgggaagcg catcgcctcg
     5341 gcaggtgaag accatacgtt gcatgtgtgg gataccgtct tacatccgct ctggctgcga
     5401 gcgattgttt tacgccttgg cctgggctac tacttttatc acaatggaca ctccagggcc
     5461 att
//
LOCUS       DASZAK010000033         8288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_639768, whole genome shotgun sequence.
ACCESSION   DASZAK010000033 DASZAK010000000
VERSION     DASZAK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8288
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639768"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VGT44_00540"
     CDS             <1..532
                     /locus_tag="VGT44_00540"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEV2579310.1"
                     /translation="DPEDDSRQPEGSLYRLDPDHSLHVMETNIRISNGIGWSPDRRLM
                     YFTDSPLRMIYVYDFDAASGALENRRPFVHTPTEKGVPDGLTVDSEGFIWSACWGGWK
                     ITRYDPAGKVERVLQLPVQYPTSCAFGGASLDELFITSAWTALSEEKRRQQPLAGDLF
                     RADVGVKGLEEPKFAG"
     gene            637..1206
                     /locus_tag="VGT44_00545"
     CDS             637..1206
                     /locus_tag="VGT44_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEV2579311.1"
                     /translation="MQQQVQSQQDNPIQPGERRNVVWRDGKANTGSTPDTFQEVLHDP
                     RALFRIKRSLAQMRRVISPQVEVANALVGRAGKFIPAEAEPYFADVHDHLVRAFEVLD
                     SYRDLMSGMLDVYLTTVSNRLNVVMKQLTIIATIFMPITFITGVFGQNFGHLPQVEHD
                     IGYLFWIVLAFMGLITILQIWYFKQRRWL"
     gene            1280..2323
                     /locus_tag="VGT44_00550"
     CDS             1280..2323
                     /locus_tag="VGT44_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV2579312.1"
                     /translation="MIALLLKGPGKADLVEMAIPEIGPDDVLIRSRAVGICGSDVELY
                     RGIRPEGFYRYPIVPGHEWAGEVSVVGERVQTFVPGDKVVAEGFLFCGICRNCRNGLT
                     NLCERGYDEIGFTRAGGMAEYVMVPARQVHSLPRMCSLEQAALLEPTAVVANAFLRAQ
                     PQPGDTVVVVGDGNIGLLAIQIARLFSPAAIVLIGSRPERLAMARQHGATHTVNGRED
                     DPLTLVQTLTKGQGADLVFEGGTRPEGVAMALRLAKRGGTVLLEGIAGARAPLSIESD
                     TFVLNHLSVFGFFGASSAAWTYAVQLFASGLLNLAPLISHRFALADYQMALDTLVDRQ
                     PGTLKVLLLHDSQ"
     gene            2733..3284
                     /locus_tag="VGT44_00555"
     CDS             2733..3284
                     /locus_tag="VGT44_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEV2579313.1"
                     /translation="MISNTPDSAGQQLSGEELHARQKKQLTDVKGPTVVAVGVADPFN
                     IGSIFRICEAAACKEIIFVDSTEIGVQKVKRASRHTSAMVPYQTCSMEEFMDMVEGLP
                     TLVAVEITSQSTDVFTTHLPQDVTLVVGGERYGIPEEILQHCAYAVHIPMFGINSSMN
                     VATSLGIVLYEWHRRYRTLSSKS"
     gene            3442..4872
                     /locus_tag="VGT44_00560"
     CDS             3442..4872
                     /locus_tag="VGT44_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV2579314.1"
                     /translation="MTITTQPRTIGELRASGYRVLSVKEELRKNLIEKIRRGEDIFPG
                     IIGYEDTVIPQVENAILSGQDIIFLGERGQAKTRMARNLVNLLDDVIPIIAGCEINDS
                     PYEPVCKACRDKVEELGDDVEIAWLPRDRRYGEKLATPDITISDLIGEVDPVRVAEGR
                     YLSDELTIHYGMIPRTNRGIFCINELPDLAERIQVGLLNIMEERDVQIRGYKIRLPLD
                     VYVVASANPEDYTNRGRIITPLKDRVGSEIRTHYPRVLEHEIQIMESESNHFITEGMD
                     IIFPQFMKEVIGTITQLARQSNEISQRSGVSVRVSISNFENVLSNASRRALRLKERQV
                     SPRVSDLPSIIASTSGKIELDTVGDVKEERVVQKLINSAIVNVFGEYFEAREFDQLVA
                     GFERGLNVQVGDDLPSMEYVNQLAKVGGLSKAVDKLNGRGSPASIASSVEFILEGLHL
                     NRRLNKDEVNGKTRYHSSGRRSLDRQ"
     gene            4903..7026
                     /locus_tag="VGT44_00565"
     CDS             4903..7026
                     /locus_tag="VGT44_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2579315.1"
                     /translation="MFKRFYKNRYGYSRWDGTQRIEGMDADDILDALSNDYIEDGDLQ
                     QALRRMMQDGIRSDDGRRTMGLRELMERMRNQRNQQLNRYNMASGVMDDLREKLEEIK
                     QLEREGIQRRLDGKEQTPQQGQPGQEGQEGQPSDQQPGQQGRQGGQTGGSNDDLTPEQ
                     KRKMLEMIAKRKQDFLNQLPQDIPGQIKGLSEYDFMDDEAREKFSELLASLQQQMMQQ
                     FFQGMQQSLQNMTPEDIARMREMIRDLNKMLRERQQGLEPDFDSFMQKYGDYFPGVNS
                     LDDLIEQMQQQMMAMQGIMDNLSQEQRQELQDLMEQLMGDDRIRVDMMELAQNLEAMM
                     PMEQVRTKFPFRGSESLPMNEAMRMMSRLQQMEGLEDQIQEARRLDNLEAIDSDKVKD
                     LLGDEEYQSVEQLKELMKVLEEAGYIQKKGNRWELTARGMRKIGQKALQDIFNKLKRD
                     GFGRHISPFRGTGGERLDESKPYQFGDPFLLDLEKTLMNAVHRRGTGTPVELQKDDFE
                     VYRTEFTTQSSTVLMIDLSMSMIYNGCQAAAKKVAVALESLIRSQFPRDHLFVVGFSY
                     IAREYKPEELIEMSRFDNDRGTNMAHGFMLSRQLLARQHGVNKQIILITDGGPTVWYE
                     NGEWLFGMPPNPFAEQQSLLEVQRCTREGITINTFMLEDDRWMSDFVNQMSRINHGRT
                     FYADKNNLGEYLLVDYLNSKKKLVK"
     gene            7205..7924
                     /gene="rsmG"
                     /locus_tag="VGT44_00570"
     CDS             7205..7924
                     /gene="rsmG"
                     /locus_tag="VGT44_00570"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV2579316.1"
                     /translation="MSQEDLRFIQVLHQFAPDVTDEQVEQFFLYRQILLEWNERFNLT
                     AITDPGEILVKHFLDSLTLLSVYGRREARVLDLGAGAGFPGLPLKVARPAWQVELLEA
                     TGKKVTFLRHVIETLGLSGIEALQGRAEELAHRRDYRGAFDLVTARAVAGLPTLLEYA
                     APFCRIGGRMIFPKKGDLTDELAQGSAAARQLGAALVDDVAVTMPGLDDGRRLLVYEQ
                     VRLCPPHYPRHGSVIARKALT"
BASE COUNT         1973 a         2137 c         2405 g         1773 t
ORIGIN      
        1 cgatcctgaa gatgactcca ggcaaccgga gggcagcctc taccgtcttg acccggatca
       61 ttctcttcat gtgatggaaa ctaatatacg tatctccaac ggcattggtt ggagtcccga
      121 taggcgactc atgtacttta ccgattctcc actgcgcatg atttacgtct atgattttga
      181 tgccgccagc ggcgctcttg agaatcgccg cccatttgtt catactccca ctgaaaaggg
      241 cgtacccgac ggtctcacgg ttgacagcga aggtttcatc tggagcgcgt gctggggagg
      301 gtggaagatc acgcgctacg accccgccgg gaaggttgag cgtgtgctcc agttgcctgt
      361 gcaatatccc accagttgtg cttttggagg agcatccctg gacgaactgt ttattacctc
      421 ggcctggact gcattgagcg aagaaaagag gagacaacag ccgctcgccg gagacctctt
      481 tcgggcggac gtgggagtca aggggctgga agagccaaag tttgccggat aatgcaaaac
      541 caggcgccgc tgtgctggca tataataacc agtaattgag actcacgata cgcttgaagc
      601 gtgtcaggac gaggtaatta catagggaga taacatatgc agcaacaggt gcaatcacaa
      661 caagataatc caatccagcc gggagagcga cgtaatgttg tttggcgaga tggcaaggcc
      721 aatacaggga gtacacccga cacatttcaa gaggtattgc atgacccgcg tgcgctcttc
      781 cgtatcaaac gatcactggc gcagatgcgg cgcgtgatca gcccgcaggt cgaagtcgcc
      841 aacgcgctgg tgggacgcgc cggcaaattc atccccgcag aggccgaacc ctatttcgcc
      901 gatgtacatg accacctggt acgcgcattt gaagtactgg attcctatcg tgatctgatg
      961 agcggaatgt tggatgtgta tctgaccaca gtttctaatc gactcaatgt agtaatgaag
     1021 caactcacca ttatcgccac catctttatg cccattacct tcatcaccgg tgtgttcggt
     1081 cagaactttg ggcatttgcc acaggtagag catgacattg gctatctctt ctggatcgtg
     1141 cttgctttca tgggacttat tacaatactg caaatctggt atttcaagca gcgaaggtgg
     1201 ctctaactct tcaggccaca tctcaatagc gatggcttgt atcaggattg tcgtgaatat
     1261 caatgaaaaa ggttgctcaa tgatcgcttt actcctcaaa ggccctggca aagccgatct
     1321 tgtcgaaatg gctatccctg agattggccc ggacgatgtg ctgattcgct cgcgggcagt
     1381 tggtatctgt ggcagtgacg tagaactgta ccggggcatc aggccggagg gcttctatcg
     1441 ctaccctatc gttcccggcc acgagtgggc aggcgaggtc tcagtcgtcg gcgagcgtgt
     1501 gcagacattt gtgcctggcg acaaggttgt cgccgaagga ttcctgttct gtggcatctg
     1561 ccgcaattgc cgaaacggcc tgactaacct gtgtgaaagg ggctatgacg agataggttt
     1621 tacccgagca ggtgggatgg ccgaatatgt catggttcct gcgcggcagg tacactcgct
     1681 cccgcgcatg tgttctctcg aacaagcggc ccttcttgaa ccgacggcag tagtggccaa
     1741 tgctttcctg cgagcgcagc cgcaacccgg cgatacggtg gtggtggtgg gcgatggcaa
     1801 tatcgggctg cttgccatcc aaattgcacg gctgttcagc ccagctgcca ttgttctgat
     1861 agggtcgcgc ccagaacgac tggcgatggc gcgccaacat ggcgcaaccc ataccgttaa
     1921 tggacgggag gatgacccac tgaccctggt acaaacactc acgaaagggc agggtgccga
     1981 cctggtcttc gagggaggca cacgcccaga aggtgtagca atggctttac ggctggcgaa
     2041 acgcggcggc accgtattgc tggaaggcat tgccggcgct cgcgctccac tgagcataga
     2101 aagcgatacg tttgtcttaa accacctgtc ggtgttcggc ttcttcgggg cgagttcagc
     2161 cgcgtggaca tatgccgtac agctcttcgc ttccggcctc ttaaatctgg cgccgctcat
     2221 ctcccaccgc tttgccctgg cagactacca gatggccctg gatacgctcg ttgatcgcca
     2281 accggggacg ctgaaagttt tattgctcca tgattcacaa tgaatacttc atgcggccag
     2341 tatccgtagg ggtattatgt caatcaccat ttgaagtagc ctgatggcga ccctatagtt
     2401 caaaatattg gaggaggaaa atggaatggc tccgcgtcat agagctactg aaacatatcg
     2461 agataaccag ggtaatatct ggactattga atacgtaagc tacgatagca ctagcggtaa
     2521 tccgggtgat tatgacgaag taatgcagtt gaagaaaaat gggagagagg ttggacatat
     2581 cacttatcat aaggataaat ctgggagaaa caaaattcca cctcgacaga ccggtagcgt
     2641 gagtttaccg tccgatgcga aaagggtagt ataatatcgg ataagagtcg ggattatcaa
     2701 aagggttgcg ataaagagca gtggaatata atgtgatctc caatacgcct gatagcgccg
     2761 gtcagcaact cagcggtgag gaactccacg cgcgccagaa gaagcagtta accgatgtga
     2821 aaggtcctac tgtcgtagcg gtgggagtag cagacccctt taatatcggg tctatctttc
     2881 gcatttgtga agcagccgcg tgtaaggaaa tcattttcgt tgactctacc gaaattggcg
     2941 tacagaaagt gaaacgcgcc tcgcgacata cgagcgcgat ggttccgtat caaacttgtt
     3001 caatggaaga gtttatggac atggttgagg gactgccgac gctggttgcg gttgagatta
     3061 cctcgcaatc cacagatgtc tttacaacgc atcttccgca agatgtcacg ctggttgtgg
     3121 gtggcgaacg ctacggtata ccggaggaga ttttgcagca ctgcgcctac gctgtgcata
     3181 tccccatgtt tggcatcaac agctcgatga atgtcgcaac ctccttgggc attgtcctct
     3241 atgaatggca ccggcgatat cgtactctct ccagcaaatc ctgacctcat gaacacttgc
     3301 gctaacatgc aatggtagaa tagaatacat atggttccac aagttactgg ccaggtggga
     3361 tcatggagga gctatactta ccttatcgct acacatgcat cacacgtgcc aacccccaag
     3421 aaaggtagca gaaaaagcgg aatgaccatt actacccaac ctcgcacgat tggcgaattg
     3481 cgagcaagtg gatatagagt actttctgtc aaggaagagc tacgtaaaaa tctgattgag
     3541 aagattcgca ggggcgaaga tatctttccg ggcatcattg gttacgaaga tacagtgata
     3601 ccgcaggtcg aaaatgccat actttccggc caggatatca tctttctcgg cgagcgcggc
     3661 caggcgaaga cgcgcatggc gcgtaacctc gtcaatctgc tggacgatgt catacccatc
     3721 atcgcgggat gcgagattaa cgatagtcct tacgaacctg tctgcaaggc atgtcgcgac
     3781 aaggtagagg agctggggga tgatgtcgag atcgcctggc tgccgcgcga ccggcgctat
     3841 ggcgaaaaac tggcgacgcc ggatatcacc atctccgacc tgatcggcga ggtagacccg
     3901 gtgcgcgtgg cggaaggccg ctatctctca gacgaactga ccattcacta cggcatgatt
     3961 cctcgcacga atcgtggtat cttctgcatc aacgaactgc cggacctggc ggagcgcatt
     4021 caggtgggct tgctgaacat catggaggag cgcgatgtgc agattcgcgg ctacaaaatc
     4081 cgcctgcctc tggatgtgta cgtcgtggcc agcgccaacc cggaagatta caccaaccgg
     4141 ggacgcatca tcacgccctt gaaggaccgc gtcggttccg aaattcgcac ccattacccg
     4201 cgcgtccttg aacatgaaat ccagatcatg gagtctgaga gcaaccactt catcacggaa
     4261 ggtatggata tcattttccc gcagttcatg aaggaagtga ttggcaccat cacgcagctt
     4321 gcgcggcaga gcaacgaaat cagtcagcgt tctggcgtga gcgtgcgcgt ctcgatctcc
     4381 aactttgaaa atgtgctgag caatgccagc cgccgcgcgc tgcgcctgaa agaacgccag
     4441 gtgtccccgc gtgtcagcga cctgccctcc atcatcgcct cgaccagcgg caaaatcgag
     4501 cttgatacgg tcggcgatgt gaaagaagag cgcgtggtgc agaagctcat caacagtgcg
     4561 attgtgaatg tctttggcga gtacttcgag gcgcgcgagt ttgaccagct cgtggcaggc
     4621 ttcgagcggg gcctgaatgt ccaggtaggc gatgatttac cgtcgatgga gtatgtcaac
     4681 cagcttgcaa aagtcggtgg actgagcaag gccgtcgata agctgaacgg gcgtggcagc
     4741 cctgccagca tagcgtcttc cgtcgagttc atcctcgaag ggctgcatct caatcgtcgg
     4801 ctgaacaaag atgaggtcaa tggcaagacg cgctatcatt cttctggtcg gcgatcgttg
     4861 gacagacaat aacaggcacg cgacacataa aggaaccttc ccatgttcaa acgcttctac
     4921 aaaaaccgtt acgggtattc gcggtgggat gggacgcagc gcatcgaagg aatggacgcc
     4981 gatgatatcc tggacgcgct ttcgaatgac tatatcgagg atggcgatct gcaacaggcg
     5041 ctccggcgca tgatgcagga cggcatccgt tcggacgatg gtcggcgcac gatgggactg
     5101 cgcgagttga tggagcgtat gcgcaaccag cgcaaccagc agctcaaccg ctacaacatg
     5161 gcctccggcg tgatggatga tctgcgcgag aagcttgaag agatcaagca gcttgagcgc
     5221 gagggcatcc agcgccgcct ggatggaaaa gagcaaactc ctcagcaggg gcagcccggt
     5281 caggaaggac aggagggcca accttctgac cagcagcccg gtcaacaagg ccggcagggt
     5341 ggacagacgg gcggtagcaa cgacgatctg accccggaac agaaacgcaa gatgctggag
     5401 atgattgcga agcgcaaaca agatttcttg aaccagctcc cgcaggatat ccccggccag
     5461 atcaaagggc tgtccgaata cgacttcatg gatgatgagg cgcgcgagaa gttcagcgag
     5521 ttgctggcca gtctgcaaca gcagatgatg cagcagttct tccagggaat gcagcagtcc
     5581 ctgcaaaaca tgaccccgga agatatcgcc aggatgcgcg agatgatccg cgacctcaac
     5641 aagatgctac gcgagcgcca gcaggggctt gagccggact tcgactcgtt catgcagaag
     5701 tatggtgact acttccccgg cgtcaattcg ctcgatgacc tgatcgagca gatgcagcag
     5761 caaatgatgg cgatgcaggg catcatggat aacctgtcgc aggagcagcg gcaggaattg
     5821 caggacctca tggaacagct catgggggat gatcgcattc gtgtcgatat gatggaactg
     5881 gcgcaaaacc tggaagccat gatgccgatg gagcaggtgc gcacgaagtt ccctttccgg
     5941 ggtagcgagt cgctgcccat gaacgaggcg atgcgcatga tgtcgcgttt gcagcaaatg
     6001 gagggattgg aggatcagat ccaggaggcg cgccgcctgg ataacctgga agccatcgat
     6061 agcgacaagg tgaaagatct gctcggcgac gaggaatacc agtcggttga gcagctcaaa
     6121 gagttgatga aggtgctgga agaggcgggc tacatccaga agaagggcaa tcgctgggag
     6181 ttgaccgcgc gcggtatgcg caaaattgga cagaaggcgc tccaggacat ctttaataag
     6241 ctcaagcgtg atggctttgg tcggcatatc agcccattcc gaggaaccgg cggcgagcgc
     6301 ctggacgaaa gcaaaccgta tcagttcggc gatccatttc tgctcgacct ggaaaagacg
     6361 ctgatgaacg cggtccacag acgcggcacc ggcactcctg tcgagctaca gaaggatgat
     6421 tttgaggtct atcgcaccga gttcaccacg cagtcgtcta cggtgctgat gattgatttg
     6481 agtatgagca tgatctacaa cggctgccag gctgccgcga agaaggtcgc ggtagctctg
     6541 gagagcctga tccgctcgca attcccgcgc gatcatctct ttgtggtcgg cttctcgtac
     6601 atcgcccgcg aatacaagcc cgaagagttg atcgagatga gccgtttcga caacgatcgc
     6661 ggcaccaaca tggcgcacgg cttcatgctc tcgcggcaac tgctggcgcg ccagcacgga
     6721 gtgaacaagc agatcatcct gattaccgac ggcggtccga ccgtctggta cgagaatggc
     6781 gaatggctgt tcggtatgcc gcccaatcca tttgccgagc agcaatccct tctcgaagtg
     6841 cagcgctgca cccgtgaggg catcaccatc aacactttta tgctcgaaga tgatcggtgg
     6901 atgtctgatt ttgttaacca gatgtcgcgg atcaatcatg gccgcacctt ctatgccgac
     6961 aagaataatc tgggcgagta cctgctagtc gattacctga acagcaagaa aaagctggtc
     7021 aagtaataat tgttgactgg tggcgtaatc aaaagtggtg acgaaagggc gaccgcaagg
     7081 gtacgccctt accatgacta cgaagtgatt cagcacgtcg ctaaacgtcg ggtcatggta
     7141 agggcgtacc cttgcggttg ccctccggga atttaccaac ggtattattt caagggtgta
     7201 tgttatgtcg caagaggact tacgctttat tcaggttcta caccagttcg cgccggatgt
     7261 tactgacgag caggtcgaac agtttttcct gtaccggcag attttgctcg aatggaatga
     7321 acgcttcaac ctgacagcca tcaccgatcc tggcgaaatt ttggtgaagc atttccttga
     7381 ttcgctaacg ctactgagcg tctatggccg gagggaggcg cgcgtcctcg atcttggcgc
     7441 gggcgcgggc tttccagggc tgccgctcaa ggtcgcgcgc ccggcatggc aggtcgagct
     7501 gttggaggcg acggggaaga aggtgacatt cctgcggcat gtgatcgaga cgcttggcct
     7561 gagcgggatc gaggcgctac aggggcgcgc cgaggagctt gcccatcgac gagactaccg
     7621 gggcgcgttc gacctggtga ctgcgcgcgc cgtggccggg ttgccgacgt tgctggaata
     7681 tgccgcgcct ttttgtcgca ttggtgggcg tatgatcttc ccgaagaaag gcgacctgac
     7741 cgacgaattg gcgcagggca gcgctgccgc gcgccagctt ggagcggcgc tggttgatga
     7801 tgtggctgtg accatgccag ggctggatga tggtcgccga ctgctggtgt atgaacaggt
     7861 caggctttgc ccgccgcact acccgcgcca cggctcggtg atagccagga aggccctgac
     7921 ttaattggta aataccctac gaagtgggcg gatttggccc ccaaacgtgg aacattgccc
     7981 tcaatcctgg acactgggac aaacttttct agaaaagccg ctcctcatct agcttctttc
     8041 atcgatgttt tgtaaatgag cgctctacaa cttcttacct ctcacaaaag gcgcgttggg
     8101 ctggtctctc gttcgttgac aggacggacg acatattata gaatggacgc atggcgctta
     8161 aacgaatgga caacgtcggc atcgtggtgg aatccctcga tgccgctctc tcttttttcg
     8221 ctgagctcgg cctcgagctc gaagggcgag ccatggtaga aggcgagtgg gcagggcgcg
     8281 tcactgga
//
LOCUS       DASZAK010000034         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_249314, whole genome shotgun sequence.
ACCESSION   DASZAK010000034 DASZAK010000000
VERSION     DASZAK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249314"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(372..1523)
                     /locus_tag="VGT44_00575"
     CDS             complement(372..1523)
                     /locus_tag="VGT44_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEV2579317.1"
                     /translation="MPAFNGRIAIVGVAESDYGRVPDMTEMQLHAQALQRVLEDSGLR
                     KEDIDGVFCSSHTLGMPTVMLCEYLQLFPRYSDTTTIGGSSFEAHLNHAVAAIRAGKS
                     EVALISYASNQLSSRGRMLGTGARPPTIPEATYETPYGNTLVGAYAMAARRHMHQYGT
                     TSEQLAEIAVVTRRHAGLNPLAMYREPITVQDVLNSRLIADPLHLLDCCVVSDGGGAV
                     LVTTEHRARDLKQTPVFVLGSSESHTHAHISQMPDLTVTAAAVTAPRAFAEAGVTPGE
                     IDMAMIYDSFTITVLLLLEDLGFCKKGEGGAFVQDGRIALGGQLPINTDGGGLSSNHP
                     GMRGIFLIIEATRQLRGQCGSRQVKGAKLAVAHGSGGLLSSQATTILGI"
     gene            complement(1724..2077)
                     /locus_tag="VGT44_00580"
     CDS             complement(1724..2077)
                     /locus_tag="VGT44_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV2579318.1"
                     /translation="MARTNFSSAVQSASEIEITVTGRRSGRSLSYPVWFALEGEKLYL
                     IPVKGSDTDWYKNLRKTPTIRLAARGKTFTTSARFLTDEAQLGKVLEKFRDKYGRNVK
                     SYYPKLDVAVEVPLE"
     gene            complement(2119..>2409)
                     /locus_tag="VGT44_00585"
     CDS             complement(2119..>2409)
                     /locus_tag="VGT44_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV2579319.1"
                     /translation="AMNMAIHMTRPGRSASLVGFTHVSQAPDLTYRLFQNITITGGVA
                     PARAYLPALLQDVIAGKLDPSPVLDITVDLAGVPAGYAAMDSREAIKVMVLP"
BASE COUNT          507 a          637 c          711 g          554 t
ORIGIN      
        1 cacttcgcgg gcgaacactg ctcggtagaa ttccagggct ttatggaagg cgcagcccgg
       61 gaaggcaagc gagccgccca cgaaatttta caagatttga cctgactcgc gtaggggcaa
      121 gcctttgcta cattgattcc aacatattgg tccaggagca ttctaagggt tgacgagcat
      181 gcagatggaa gggagcgcac ttgcttgtca tgctgagcgc cagcgaagca tctcacgcgt
      241 cgggcaccga gatgcttcgc tgcggctcag catgacaggg gtggccgatt cgtctttctt
      301 cgctagaggt gctgaacgtg caattagttg gaatcaatag gggtaagccc cgcccctaca
      361 atatacggga ttcagattcc tagaatcgtt gtcgcctgcg acgataacaa tcccccgctc
      421 ccgtgagcta ctgctagctt tgcgcctttc acctggcggg agccgcactg gccgcgcaat
      481 tgacgggtgg cctcgatgat caggaaaatg ccgcgcatac cgggatggtt ggaagaaagc
      541 ccgcctccat cagtgttgat tggtaattga ccacccaacg cgatgcgtcc gtcctgcacg
      601 aatgctcccc cctcgccttt cttgcaaaag cccaggtctt caagcagcaa tagcacggtg
      661 atagtaaaag aatcgtagat catggccata tcaatctcac ctggtgttac tccagcctct
      721 gcgaaagctc gcggcgcggt aaccgcggcg gctgtgacgg tgagatctgg catctgcgag
      781 atgtgggcgt gggtatggct ctcgctgcta cccagtacga atacaggcgt ttgcttcagg
      841 tccctggctc gatgttctgt cgtaaccagt acggcgcctc cgccatcgct gacgacgcag
      901 cagtcgagca ggtgcaacgg gtcggcaatt aaacgcgaat tgaggacatc ctgtacggtg
      961 attggttcgc ggtacatggc aaggggattg aggcccgcat gcctgcgtgt tacgacagca
     1021 atctcggcga gttgttcgga ggtggtgcca tactggtgca tatgcctgcg ggctgccatt
     1081 gcgtacgcgc cgacaagtgt attaccgtaa ggagtctcgt aggttgcttc gggaatggtt
     1141 ggcggtcttg ctcccgtgcc aagcatacgc ccacggctgg agagctggtt actggcgtat
     1201 gagatcagtg caacttcgct cttgcctgcg cggattgccg ctactgcgtg gtttagatgt
     1261 gcctcgaagg aggaaccacc gatggtagtg gtgtcgctgt aacgcgggaa taactgcaag
     1321 tattcgcaga gcatcacagt tggcatgcct agagtatgcg aactacagaa tacgccatcg
     1381 atatcttctt tgcgcagtcc gctatcctct aacacgcgct ggagggcctg tgcgtgcaat
     1441 tgcatttcag tcatgtcggg aaccctgccg taatcggatt cggctactcc gacgatggca
     1501 atgcgtccgt tgaatgcggg cattgaatgg atgctccttt gcctgtttga tgatgctcgt
     1561 atcgttttga aatctgcaag gcagtccggg tgacccgaaa tgtggtggag gccctcttcc
     1621 ggaggatgac ccgatctccc cgcttgatgg tcgtcacctg cgaggtgatg gaatgagaaa
     1681 cagctccaga aagagcaacg acatgccaga ccgtgctggc acctcactca agggggactt
     1741 cgacggccac gtcgagcttg ggatagtagg acttcacgtt tctcccgtac ttgtctcgga
     1801 acttctcaag gaccttgccg agctgcgcct cgtcggtgag aaaacgggcg ctcgtggtga
     1861 acgtcttccc tctggcggcc aggcggatgg tcggtgtctt gcgcaggttt ttgtaccagt
     1921 cggtatctga tcccttcact ggaatcagat agagcttttc gccttccagg gcgaaccaaa
     1981 cggggtagga aagggaacga ccagaccttc gaccggtcac ggtgatctcg atttcgctgg
     2041 cgctctgcac ggcagagcta aaatttgtgc gtgccatagg agtctccttg ttggttgatt
     2101 gaggaataac gctggtttct acggaagcac catcactttg atggcttcac gactatccat
     2161 cgccgcgtat ccagcaggaa ccccagcaag atcgactgtt atgtcgagga caggtgaggg
     2221 atcaagtttt cctgcgatca catcctgcaa caatgctggc aggtaggcgc gcgcaggtgc
     2281 tacgccgccg gtgatggtaa tattctggaa caatcggtac gtcagatcag gagcctggct
     2341 gacatgggta aaaccaacga gactggctga tctccccggt cgggtcatgt gaatcgccat
     2401 gttcatggc
//
LOCUS       DASZAK010000035         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_178140, whole genome shotgun sequence.
ACCESSION   DASZAK010000035 DASZAK010000000
VERSION     DASZAK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178140"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="VGT44_00590"
     CDS             complement(<1..1172)
                     /locus_tag="VGT44_00590"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEV2579320.1"
                     /translation="MTAIFRSLKRAYDLRLSVKCLALLLLFGAALLLERIAGGFPPPA
                     WRVLAQAAPQLSSLFSTRGLPVFAPFAGLLVLSTTWAALWLVLAWTALVLVWRAWFPA
                     RRTHSQAIRWQRTSFDNLLDSGFDEPQATLAYASAYDSAYTSFDQVASPSTPFPDIPT
                     HPEPRPHPASQQAPNPVGARRTGGADLNGHPTEEGHYSDRPPKQERRASRPPQKARPG
                     SRPPRGEHPRPRPMADNPKGQPTTRQTTVILTDVETRTKAAEPTASTWPTDATQKVGI
                     GWNAGITRKNKPNEDSVLALHSACPFRGQLMPFSLYAVADGMGGHANGQVASHLATHS
                     LMQSVLPAVQHGEELTEEFLIETMVDCAHLANQAVFQFREDHATDLGTTLTAALVA"
     gene            complement(1174..>2056)
                     /locus_tag="VGT44_00595"
     CDS             complement(1174..>2056)
                     /locus_tag="VGT44_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579321.1"
                     /translation="HRLTRLAQPVKPRKPRYLSKRTLRTGVVVLGLILLIDIITARYF
                     PQVQSALTLPSQTGHANIGGVVQVPVMKNGVTATTARGTTTSATTTPVAQVTRTATTT
                     PALKQLAQDTFARPDQPLWGTASDGQAWSSDAMQAPVFSIINHLGLVNNGSGIYDAIL
                     GPRAANSEVEFSGTVSHFASANMGALLRWTDANNLYKVFIDGANLVALKKVNGVVTVL
                     QSVPFGAKDGTSYTIRARVMGTSLLARAWPTGQAEPTTWMLTATDGDLASGFDGMRLI
                     VQNGITIAISSFLESQV"
BASE COUNT          370 a          593 c          686 g          407 t
ORIGIN      
        1 tcggccacca gcgcggcggt gagggtggtg ccaagatcgg tagcgtggtc ttcgcgaaac
       61 tggaaaacgg cctggttcgc caggtgcgcg caatccacca tcgtctcaat caggaactcc
      121 tcggttagct cttcgccgtg ctggaccgcc ggcagtacgc tctgcatcag gctgtgcgtg
      181 gcaaggtggc tggcgacctg gccgttggcg tgtccgccca tgccgtccgc tacagcgtag
      241 agggagaacg gcataagttg ccctcgaaat gggcaggcac tatgcagcgc aagcacggaa
      301 tcctcgttcg gcttgttttt gcgtgtgata ccggcgttcc agccaatgcc caccttttgt
      361 gtcgcgtccg tcggccatgt cgaagctgta ggctcagccg ccttcgttct cgtttccaca
      421 tcggtcagga taacggtggt ctggcgcgtg gtaggctggc ctttgggatt atccgccatc
      481 gggcgtgggc ggggatgttc tccgcgcggc gggcggctgc ctggccgggc tttctgcggc
      541 gggcgactcg cgcgccgttc ctgtttcggt ggacgatctg agtagtgtcc ttcctcggtc
      601 ggatgaccat tgagatctgc cccgcctgtt cgcctcgctc caacaggatt tggagcctgt
      661 tgagaagcag gatggggacg gggttcagga tgggtcggta tatcggggaa aggtgttgaa
      721 ggcgaggcaa cctggtcgaa tgaggtataa gcggaatcgt aggccgaagc ataagcaagc
      781 gttgcctgtg gctcgtcaaa gccggagtcg agcaggttgt cgaaggaggt gcgctgccag
      841 cgtatcgcct ggctgtgggt gcggcgcgcg ggaaaccagg cgcgccagac cagaacgaga
      901 gccgtccatg ccagcaccag ccagagcgca gcccacgtcg ttgatagcac cagcaagccc
      961 gcgaagggcg cgaacaccgg cagtcctcgc gtggagaaaa ggctggaaag ctgtggagcg
     1021 gcttgcgcca gaacgcgcca ggccggtggt ggaaagccgc ccgcgatgcg ttcgagcagc
     1081 agcgccgcgc caaagagcag cagcagggca aggcacttca ccgatagacg tagatcatac
     1141 gcgcgtttga gggatcggaa tatagcagtc atgttagacc tgcgattcca ggaatgagct
     1201 gatggcgatg gtgatgccat tttgcacaat caggcgcatg ccgtcgaagc cagatgccag
     1261 atcgccatcg gtggcggtca gcatccaggt cgtcggttcg gcctggcccg ttggccacgc
     1321 gcgcgccagc aggctcgttc ccatcacgcg cgcgcgtatc gtataagatg tgccatcctt
     1381 cgccccgaac ggtaccgatt gcagcaccgt caccacgccg ttgacctttt tgagcgcaac
     1441 caggttcgcg ccatcgatga agaccttgta cagattgttc gcatccgtcc agcgcagcag
     1501 cgcgcccatg ttcgcggaag caaaatgact gaccgtgccg ctgaactcga cctcgctatt
     1561 ggcggcgcgc gggccaagaa tagcgtcgta gatgccgctg ccgttgttga ccagccccag
     1621 gtgattgatg atagaaaaga ccggcgcttg catggcgtcc gagctccacg cctggccgtc
     1681 cgatgccgtt ccccacagcg gctgatctgg gcgcgcgaag gtgtcctgcg ccaattgctt
     1741 gagggcgggc gtggtcgtcg cggtcctggt tacctgcgct acaggcgtag ttgttgccga
     1801 tgtggtcgtg cctcgcgcgg tggtggctgt gaccccgttc ttcatcaccg gcacctgcac
     1861 gactccgcca atgttggcat gtccggtttg cgagggcagg gttaaggccg attgcacctg
     1921 gggaaaatac ctggctgtga tgatatcgat cagcaggatc aagcccagca cgaccacgcc
     1981 ggttcgcagc gtgcgctttg aaagatagcg tggcttgcgc ggcttgactg gttgtgccag
     2041 ccgcgtgaga cgatgt
//
LOCUS       DASZAK010000036         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_533195, whole genome shotgun sequence.
ACCESSION   DASZAK010000036 DASZAK010000000
VERSION     DASZAK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533195"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VGT44_00600"
     CDS             <1..838
                     /locus_tag="VGT44_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HEV2579322.1"
                     /translation="FESMVCKAQEYIRAGDIFQVVLSQRLSRRVNAAPFTVYRALRSI
                     NPSPYMFFLDLQDFHIIGASPELLVRVEDGEVTIHPIAGTRKRGNDPQSDLQLAEELK
                     NDPKERAEHVMLVDLGRNDVGRVSIPGSVKVSEFMEIERYSHVMHLVSNVTGQLRSEL
                     TPYDALRAGFPAGTVSGAPKIRAMEIISELEGEQRGVYAGAVGYFSHSGNLDTAITLR
                     TMVIRDGLAYIQAGGGIVADSNPSEEYQESMNKAKALLRALDEAEQISTERRETYVVT
                     HR"
     gene            819..1385
                     /locus_tag="VGT44_00605"
     CDS             819..1385
                     /locus_tag="VGT44_00605"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV2579323.1"
                     /translation="MLLLIDNYDSFTYNLYQYLSELGADVEVRRNDQVSIGEIASMGP
                     DHIVISPGPCTPAEAGMSCQIIESFGPCTPVLGVCLGHQAIGQAYGGRIQRAPEPMHG
                     KTSLMYHAGQGVFRNLPIPFEANRYHSLIVERSTLPAELEITAETTDGLIMGLRHRTY
                     PVEGVQFHPESIMTPIGKDLLRNFLLMH"
     gene            1403..2446
                     /gene="trpD"
                     /locus_tag="VGT44_00610"
     CDS             1403..2446
                     /gene="trpD"
                     /locus_tag="VGT44_00610"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV2579324.1"
                     /translation="MIREAISHVTTGATLSEGEAAVVMEEIMTGVATQAQMGAFLTAL
                     HLRPGSETVQEITGLARVMRQKAVRVHLKDHIATQAIDTCGTGGDRAGTFNVSTAAGI
                     LAAAAGASVAKHGNRSATSRCGSADVLEALGVRIDLGPEQVSQCVQEVGFGFMFAPAY
                     HPAMKHVGPARREIGIPTVFNILGPLTNPAHTRYQVLGVADGSLLRTMGEALLHLGCK
                     HALIIHGEDGIDECSMSASTRICEVRKGEDLREYTITPEEVGLTSVTDRRSFQGATPE
                     YNANLLRDLLSGQNHGPAADMICLNAGAALLANELVTSFSDGVKLARSTLREGKASRK
                     LQDVVTYTQMLAG"
     gene            2450..>3529
                     /gene="trpC"
                     /locus_tag="VGT44_00615"
     CDS             2450..>3529
                     /gene="trpC"
                     /locus_tag="VGT44_00615"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF001377.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV2579325.1"
                     /translation="MFLDQIVSRTRADLEQRKCLQPLEDLQRLALAQAAPRDLLEALR
                     PRSKAKISLIAEVKRASPSKGVFASHVDPVALARTYAANGAAAISVLTEPHFFLGSFE
                     YLPAIKQAVNVPVLCKDFIVDEYQVYEARAWGADAILLICAILDQVQLRRLLNVAHDL
                     RMRCLVEVHTSGEVERAIDAGAMVIGVNSRDLKTFQVNPHLIRALRPLIPADRVVVAE
                     SGIHTSSDARRLARCDVQAMLVGESLVTSHDIPAQMRTLLEGANESVQVKICGLRTKV
                     QWLSAIDAGADLLGLMFYKPSSRYIEPREAKALLNNLEEGQITSEIVGIFVNEEAEFI
                     NDTIEQVGLHYVQLHGDESPEFCERI"
BASE COUNT          834 a          925 c          962 g          808 t
ORIGIN      
        1 attcgagtca atggtgtgca aagctcaaga atacattcgc gcaggtgaca tcttccaggt
       61 cgtactctca cagcgcttga gccgtcgtgt caatgcagcg ccatttaccg tgtatcgtgc
      121 cctacgttcc atcaatccct cgccctacat gttctttctg gatttacagg attttcatat
      181 cattggcgct tctcccgaat tactggtgcg cgtcgaggac ggagaagtca ctattcatcc
      241 cattgctggt actcggaaac gaggcaacga tccccagagt gacctacagc ttgccgagga
      301 attgaaaaac gatccgaaag aacgcgcgga acatgtcatg ctggtcgatc ttgggcgcaa
      361 tgatgtcgga cgagtgagta ttccaggcag cgtcaaggtg agtgagttta tggaaatcga
      421 gcgctattcc cacgtcatgc accttgtctc caacgttacg ggtcagctac gatctgaact
      481 cactccctat gatgcccttc gagcgggttt tcccgccggt acagtctcag gagcgccgaa
      541 gattcgggct atggagatca ttagcgaact ggagggagag cagcgaggag tctatgctgg
      601 agctgtaggc tacttcagcc attcaggcaa cctggatacc gctatcacac tgcgcaccat
      661 ggtcatccgc gatggtctcg cctatataca agccggaggt ggtatcgtcg ccgattccaa
      721 cccttccgaa gagtatcaag aaagcatgaa taaggcgaaa gcgctgttac gtgcccttga
      781 cgaagccgaa cagatttcta cagagcggag ggagacgtat gttgttactc atcgataatt
      841 acgatagctt cacctacaac ctctatcaat acctttcaga actgggggcc gatgtagaag
      901 tgcggcggaa cgaccaggta agcataggtg aaatcgcgtc aatgggcccc gatcatattg
      961 tcatctctcc agggccatgt accccggcag aggcgggcat gtcctgccag attattgaat
     1021 catttggacc atgcactccc gtgctcgggg tatgtcttgg gcatcaagcc ataggacagg
     1081 cttatggcgg gcgcatccag cgcgctccgg agccaatgca tggcaaaacc tcattgatgt
     1141 accacgcggg acagggggtc tttcgcaacc tcccaatccc ctttgaagcg aatcgttatc
     1201 atagcctcat tgttgagcgc tcaaccctcc cggctgaact ggagatcacc gccgagacga
     1261 cggatggttt aatcatgggt ctgcggcatc gcacctaccc cgttgaagga gtgcagttcc
     1321 accccgagtc aatcatgact ccaataggaa aagatctgct acgtaatttc ctgcttatgc
     1381 attgaaccga gaggagaaag aaatgatccg tgaagcaatc tcgcacgtca caaccggagc
     1441 caccttgagt gaaggtgagg cagccgtagt gatggaagaa attatgacag gtgttgctac
     1501 ccaggcacaa atgggagctt ttttgacggc cctgcacctg cgccctggca gcgagaccgt
     1561 tcaagaaatc accgggctgg cgcgtgttat gcgtcagaag gctgttcgag tacacttgaa
     1621 agatcatatt gcaactcagg cgatagacac gtgtggcact ggtggcgata gggccggcac
     1681 atttaatgtt tcaaccgcgg cgggtattct tgcagcggca gcaggcgcct cggtggcaaa
     1741 acatggaaac cgctccgcca ccagccgctg cggcagcgcg gatgtgttag aggcattggg
     1801 agtgcgtatt gatctcggtc ctgagcaagt atcgcaatgc gtgcaagaag tcggctttgg
     1861 cttcatgttt gccccagcgt atcatccggc catgaaacat gttggaccgg cgcggcgtga
     1921 gatcggcata cctaccgtat tcaatatact tggcccattg acgaacccag cacacacccg
     1981 ctaccaggta cttggagttg cggatggttc gctgctgcgt accatgggtg aagcattact
     2041 gcatcttggt tgcaaacatg ccctcattat tcatggagaa gatggcatcg atgaatgttc
     2101 tatgagcgca tccacccgca tatgtgaggt gcgcaagggc gaggacctgc gtgaatacac
     2161 catcacgccg gaggaggtgg gactcaccag tgtgactgac cggcgctcat ttcaaggcgc
     2221 tactcccgaa tacaacgcaa atttgctaag ggacttattg agcgggcaga accatggccc
     2281 tgctgccgat atgatttgct tgaatgctgg cgctgcgctg ctggcaaatg agctggtaac
     2341 ctccttttct gatggagtca agttagcccg ctcaacgctg cgtgaaggca aagccagccg
     2401 caaactgcaa gatgttgtca cctataccca gatgttggca ggctgaacta tgtttcttga
     2461 ccagatcgtc tcgcggacaa gggccgacct tgaacagcgt aaatgcctgc agcccctgga
     2521 ggatctacaa cgccttgcat tggcgcaagc agcgcctcgc gatctattag aggcgcttcg
     2581 gcctcgctcg aaagcaaaga tcagcttgat tgccgaggtc aaacgcgctt caccctcgaa
     2641 gggggtgttc gcgtcacatg ttgatcccgt cgcgctggcg cgcacgtatg ccgcaaacgg
     2701 agctgctgcg atttctgtgc tcaccgagcc tcatttcttc ctgggttcat ttgaatacct
     2761 cccggctatt aaacaggcgg tcaacgttcc ggtgttgtgc aaagatttca ttgttgacga
     2821 ataccaggtg tatgaagcgc gagcctgggg cgcagatgcc atcctactca tctgcgcaat
     2881 cctggatcag gtccagctac ggcgcttgct caacgtagcc catgacctgc gtatgcgttg
     2941 cctggtcgag gtgcatacct ctggtgaggt agagcgagca atcgatgccg gagcaatggt
     3001 aatcggcgtt aacagtcgtg acctcaaaac ctttcaggtg aatcctcatc ttatccgagc
     3061 attacgacca ttgattcctg cggatcgtgt tgtagtggcg gaaagtggca tacataccag
     3121 cagcgatgca cgtcgcctgg cccgctgcga tgtacaggcc atgctggtgg gtgaatcgct
     3181 ggttacatct catgacatac ctgcgcagat gcgtacactt ctcgagggag cgaatgaaag
     3241 cgtgcaagtg aaaatctgcg gcttgcgcac caaagttcaa tggctgtcag ccattgatgc
     3301 aggggctgac ctgctcggct tgatgtttta caaacctagc tcgcgctaca ttgagcctcg
     3361 agaagctaaa gcgttgctca acaatctcga agaaggtcaa atcacctccg aaatcgtggg
     3421 aatatttgtg aacgaagaag ctgagtttat taacgacact attgaacaag ttggtctgca
     3481 ctatgtacag ctacatggag atgagtcgcc tgagttctgc gaacgcatc
//
LOCUS       DASZAK010000037         2752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_284802, whole genome shotgun sequence.
ACCESSION   DASZAK010000037 DASZAK010000000
VERSION     DASZAK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284802"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..741)
                     /locus_tag="VGT44_00620"
     CDS             complement(82..741)
                     /locus_tag="VGT44_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579326.1"
                     /translation="MNSHQNDEFQRLLAKRAIAVAAVLCALAASAAARTITPRIDALG
                     AHVNAGRGCPECHTPRRTSSPERIPLTRKAYHGGEMLWGQDVASTYAAYGSPSDHSAQ
                     NDGSDSDEILRCLTCHDGNYGPQAMMRNVVYETLPSKYGSLGAIPTLMDQESLIKGHQ
                     IEDHPLGLDAQIKCGGALGWDCTETNGVITMNGAHSSHFAASYGFFVKPSHHGSTSVV
                     A"
     gene            1335..2279
                     /locus_tag="VGT44_00625"
     CDS             1335..2279
                     /locus_tag="VGT44_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579327.1"
                     /translation="MLEKLSRALGEPIITYWNSNKGSICQNDVTGLYALVRNLGKVER
                     LTLFVKSDGGSGQASLRMVNLLRRYVKHLTVLAPLECQSAATMLALGADRILMGPLAH
                     LSAVDTSLTHDLSPIDRDNDRVSVSNDELLRVIRLWTEQAKDSTTNPYAALFPYVHPL
                     VIGAVDRSSALSTRICEEILSYHMDDKERAREISNNLNSGYPSHNYPITLREAKRIGL
                     NVEAMDDNVNGLLFELNEVYSEMGESATTDYDEKNSHDNSIIKVMEAAGTLIYYQLDK
                     DWHYRGEERRWVALNEKSQWRRAELVKGKVTISQLHVR"
     gene            2311..>2752
                     /locus_tag="VGT44_00630"
     CDS             2311..>2752
                     /locus_tag="VGT44_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579328.1"
                     /translation="MPVRFESARFLSLLLFGIEERLMQAVWHLGAVALGVLGDVHGGV
                     GGAEQAVRRGAVVRIKGDANACPTAEHITLHGKGRVETALEALRNFFDSGSAPRNRYQ
                     GRKLVAAEAREHIAGPELPLHAHGNFLQVEVADVMAVEVIDLLEL"
BASE COUNT          556 a          743 c          871 g          582 t
ORIGIN      
        1 gtctccaggc ggaaagaggt tcgatgcggt actcgccgtt atccgcgtga tcgtcatcag
       61 gtgtggattg tggcatgtgg ttcaggcgac gacggaggtg ctaccgtgat ggcttggttt
      121 gacgaagaag ccataactcg cggcaaagtg cgatgagtga gcaccgttca tcgtgataac
      181 gccgttggtt tctgtgcaat cccagccaag tgcgccaccg catttgattt gagcgtcaag
      241 tccaagcggg tggtcttcaa tctggtggcc cttgatgagg ctttcttgat ccatcaaggt
      301 cgggatggcg ccgagtgaac cgtattttga cgggagggtt tcgtagacga cgttgcgcat
      361 catcgcttga gggccatagt ttccgtcgtg gcaggtgaga cacctgagaa tctcgtcaga
      421 gtcagagccg tcgttttgcg cggagtggtc ggatgggctt ccgtaggccg cgtaggtgct
      481 tgccacatcc tggccccaaa gcatttcccc gccatggtag gctttgcgcg tgagagggat
      541 gcgctcaggg cttgaggttc tgcgcggcgt gtgacactcg gggcatccgc gtccggcgtt
      601 gacatgtgct ccgagggcgt ctatccgagg cgtgatcgtc ctcgcagcgg cggatgcggc
      661 cagcgcgcaa agaacggctg caacggcaat cgcgcgcttc gcgagcaatc gttggaattc
      721 gtcattttga tggctgttca tataccgatc tttcgaacag tccatcatta cgccgctgcg
      781 aagggagcgg cggttgtgtg acactttcga gtggtaacga gagtcataag agctttctaa
      841 tgttgcgagt gattgagggc tgggactcta cgaatcgagt gcgctgagaa ctctctgaag
      901 cgcagagccg gggctttcct tatcacgggt ccaagcacga ttgatggact cagcagcgca
      961 ctccaatttc acagattctc gagagcgtga gcgggtcctc agagactgca ggttgagatg
     1021 cgttggcgtg ctgcttacca aagttttcga gcggctcatg ggcatctggg gtgcaagtct
     1081 ttgcagggag gcagctttgt cgcggaactc gtttcggcag gcagaagggc cactgagcag
     1141 tgcgtcaaat caccacgttc cttatttcgg cctgataagc atcgtgctaa gatctagcca
     1201 atcaagacaa ttcacttgag cggtacccgg aattgcataa ccaatctgta tctgcaaccg
     1261 catctgtttc tgagtttcca gtaatacgcc ccggcgcggc caagccgccg atgctgatgg
     1321 agcggaccca gccagtgctc gagaagcttt ccagggcgct tggtgagccg atcatcacct
     1381 actggaattc caacaaaggt tccatctgcc agaacgatgt aaccggcctc tatgccctgg
     1441 tgcgcaacct ggggaaagtg gaacggctta cgctgtttgt gaagtcggac ggcggatccg
     1501 ggcaggcctc gctgcgcatg gtgaatctgc tgcgccgcta tgtgaaacat ctcacggtgc
     1561 ttgctccgct cgagtgccag tcggcggcca ccatgctggc cctgggtgca gatcgcatcc
     1621 tgatgggtcc gctggcgcac ttgtcggccg tcgacacttc gctgacgcac gatctttcgc
     1681 ccatcgaccg tgacaatgac cgcgtgagcg tgagcaacga cgaactgctg cgcgtgatcc
     1741 ggctgtggac cgagcaagcg aaagactcga cgaccaaccc gtacgccgcg ctattcccat
     1801 acgtccatcc gctggtgatc ggcgcggttg atcgttccag tgcgctttca acgcgtatct
     1861 gcgaggagat tttgtcgtat cacatggacg acaaggagcg ggcgcgcgag atcagcaaca
     1921 atctgaattc cgggtacccg tcgcacaatt accccattac tctgcgcgag gcaaagcgga
     1981 ttggcctgaa cgtcgaggcc atggacgaca acgtgaacgg gctgctgttt gagctgaacg
     2041 aggtctactc cgagatgggc gagagcgcga ccaccgacta cgacgagaag aattcgcacg
     2101 acaacagcat catcaaggtg atggaagcgg caggaacgct gatctactac cagctcgaca
     2161 aggattggca ctatcgcggg gaagagcgcc gctgggtggc tttgaacgaa aagagccagt
     2221 ggcgcagggc ggaactggtc aagggcaagg tgacgatctc gcaactgcac gtgcgctgaa
     2281 gatcggattg agaattgcaa ggggggaagc gtgccggttc ggtttgaatc ggcgcgcttt
     2341 ttatctttgc tgctcttcgg gattgaagag cgccttatgc aggcggtctg gcatctcgga
     2401 gccgtggcgc ttggtgtgct tggcgacgta catggcggcg tcggcggcgc tgagcaggct
     2461 gtccgccgag gtgccgtcgt caggataaag ggcgacgcca acgcttgccc caccgcggag
     2521 cacataaccc tccacggcaa agggcgcgtc gaaacagcgc tcgaggcgct gcgcaatttc
     2581 ttcgacagcg gctcggctcc gcgcaaccgg taccagggcc gcaaactcgt cgccgccgag
     2641 gcgcgcgagc atatcgccgg accggagctg ccgcttcatg cgcatggaaa cttcctgcag
     2701 gtagaggtcg ccgatgtgat ggccgtagaa gtcattgacc tgcttgaact cg
//
LOCUS       DASZAK010000038         7160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_426799, whole genome shotgun sequence.
ACCESSION   DASZAK010000038 DASZAK010000000
VERSION     DASZAK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426799"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1360)
                     /locus_tag="VGT44_00635"
     CDS             complement(203..1360)
                     /locus_tag="VGT44_00635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV2579329.1"
                     /translation="MRVAIITENFLPKLDGVTRTLARLLEHLQQQGHRALLLGPESGM
                     EHYAEAEIVGTAGLPLPFYPELKFNFFRPLFIRRLKEFRPDIVHLVDPVVLGAAGLAA
                     ARFLNLPIISSYHTNLAAYCGHFGFSAFTRPMWSYNRFIHNQCVLTFCPSPSTAAMLS
                     RQGFEHVRLWPRGVDTDLFQPSRRSLELRSSWLKNRADDTAILLYAGRLSWEKNLRLL
                     IQAYRGMDTTRSHLVIVGDGPAMQDAQQDLAGLPVTFTGYLSGEQLATAYASADIFAF
                     PSTSETFGQVVLEAMASALPVVGVHSEGVCDLVRHQQTGLLLNTTAETREQAEHYRAL
                     LTTLIEQASTRHIMGQAGLLEARQRSWPAAMQHLLSGYNEVIEAASPLVAA"
     gene            1750..3663
                     /locus_tag="VGT44_00640"
     CDS             1750..3663
                     /locus_tag="VGT44_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV2579330.1"
                     /translation="MPRHIAEMNAWASTFSPHDSSAGGYASPSIVDQDTVEQRAAQAG
                     SWTQVAQLPLSQQQQNQQYQRQGAAGGIVWDDAIDTAVMAIMQGPVSPSESGITPHAR
                     LYNGVSNTVNGPHSQALHPPLQQSYGQQAQYNAFAAVQPAGFVPQAQALSAPAQGQVM
                     GMPTMMAAFPTAPAYQPYQPQQQYQQYQQGMDGPPPYMQPPLPPLPPGGRHRKKRRFP
                     IWARALVGVLLFLMLLGGGGYAYYLINFSSAVGNDTNQAAARLKGDDNPNQNLNGNIL
                     SGPRVNILLLGSDTDQKFGTTAGGSTVYLAQTDIIVSIDPTTKSVVMLSIPRDFYINI
                     PGYGMGKLDEAFAHGYSYGQGGFLGGVALSRLTIYQDFGISINYYAWVGLDGFVKVID
                     TVGGVDVDVLHPITDDAYPDDVGNKTGDTYAYKRLYIPPGPQHLSGGDALDYVRSRHA
                     DLVGDFGRSARQQQVLSALKIKLANPNIIGQLPQLASDLNGWVKTDMQLPDVIKLMNF
                     ARTLDASTVQRVVLEPPYSSSTTLPSGESIVEPNCSAIVPEITKVLQLSTARCNIYAS
                     SNTTQIASSSPGARPVVNGSADIQQIAQASSQLASLNPLNLVGGSSDLFGIHSMLDLL
                     FTVVFESPQAMQV"
     gene            3680..4888
                     /locus_tag="VGT44_00645"
     CDS             3680..4888
                     /locus_tag="VGT44_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579331.1"
                     /translation="MRSTRKQKPWFSFALLLLLSLSVQGCLGFGSSTPPNSTTITQHG
                     NGGQQVTVAQNLFKGKIYLTIDHNLWVFTGNNKYTELLRSGNVYDPAVSPDGKWIAFV
                     YRFKDYSDLMVIPSGGGPERKLLDGNGHFYIDSGFPKDTYFWFVQPSWSPDGTHILFL
                     SDLQKNYVWAGLNNLFKNSYFLDLQVFSIPFNDPTATPQVLAYANFGDGGNRDAAYQP
                     EHSSTSQIIYTHYAYDAQTGTQQVIQIYMADANAIADHPGTYSPVYDSGVALTPGSAE
                     NIQPAFSPNGNFIAYIRRESATSMGLYVMSTPVGVTADPNSQTSQAQALIPYKTSSHL
                     WSSQYVSQPIWSPDGKQIAFLEYTNNEFDLWLANVSYNAQTGLFKLQGSPMQITTGGV
                     DGESRPVWTT"
     gene            5054..6025
                     /locus_tag="VGT44_00650"
     CDS             5054..6025
                     /locus_tag="VGT44_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2579332.1"
                     /translation="MTQFEGTLIAKRYEIREHIATGGMASVFKTWDHRVERLVAIKVL
                     RSLDKNDQRAVERFRREARAAAALAHPNAVTIYDFVEEFGQYFLVMEYIHGPTLKQLI
                     GQRRHLHAYETLEIAAQVCAVLQVAHARGFIHRDIKPQNIMLASSGASSPLIASAIAP
                     STSLLVKLTDFGIVRVAEDAGLTNSGIVLGTADYLSPEQARGEKLGPSSDLYSLGVVM
                     FEMITGRPPFIGPTAVSIAMQHASTDPPPLRQFTSNVPPALEQIVSKALAKEPEGRFL
                     SAAEMQYALRQCARELRGHANYITAPISNPAPSAGGYAPTHPPLYRS"
     gene            6053..6448
                     /locus_tag="VGT44_00655"
     CDS             6053..6448
                     /locus_tag="VGT44_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV2579333.1"
                     /translation="MEHTQISTPHAPAAIGPYSQAIRYGQFIYASGQVALDPASGALV
                     GTDVEAQTHRVFQNIQAVLATANTTLAHVVKTTVFLTRMSDFQDMNAVYASYFAATGA
                     PAPARSTVAVAELPRNALVEIECVAYVEA"
     gene            6458..>7160
                     /locus_tag="VGT44_00660"
     CDS             6458..>7160
                     /locus_tag="VGT44_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEV2579334.1"
                     /translation="MANNTIIWHATLDRLQDAQMDAVCRAWLQSANLASAPNFGADDI
                     DAEDLVGQEEPLYFTLHVPSPLARDVINTRWRRSIEDILTDLTGQPVTIVVSDHFYED
                     GLPYGLQAIPDSAGGRYIPGGYYSRESNPYPPTPRYAQGNDYASYDFPVRPSQELPEA
                     GSMMGGANGGSASRYIPEPAAPYYAQTDDWNNNEQRATILMQNRLNPRYTFDAFIVGN
                     SNRLAHAASLAVAEAP"
BASE COUNT         1555 a         2015 c         2102 g         1488 t
ORIGIN      
        1 tgggcgcaat gaattgcgcc cctacatgcc acatcaacca tgacagaaca ctactgttac
       61 tggaaatgag gaaagctcta tgcagaagaa aaaaaccttc agcaccatgg agatagtgct
      121 attgggcgta ctgattggct cgctcgtcct ggccttcctg caggcgtggc aggaagaaca
      181 taggtagctt acactcctac gtctatgctg ccaccaatgg gcttgcggcc tcaatgacct
      241 cgttgtagcc agagagcagg tgctgcatgg cggcgggcca ggagcgttgg cgggcttcga
      301 gcagcccggc ctgacccatg atgtggcgcg tgctggcttg ctcgataagg gttgtcaaca
      361 atgcgcggta gtgttccgcc tgttcgcgcg tttcggcagt cgtattcagc agcaaccccg
      421 tctgctgatg ccggacaagg tcgcatactc cttctgagtg gacgccgacg acggggaggg
      481 cggatgccat agcttccagg actacctggc cgaacgtttc tgaggtggag gggaaggcga
      541 agatgtcggc tgaggcgtag gcggtggcca gttgttcgcc actgaggtag ccggtgaatg
      601 tgacgggcag ccctgccagg tcttgctgtg cgtcctgcat ggcgggtcca tctccgacga
      661 tcaccaggtg agagcgtgtt gtgtccatgc cgcgatatgc ctgaatgagc aggcgcaggt
      721 tcttttccca cgatagacga cccgcgtaga gcaggatggc ggtgtcgtct gcgcgattct
      781 tcaaccagga cgagcgtagt tccaggctgc gacgggaggg ttggaagaga tcggtatcga
      841 cgccacgcgg ccacaggcgc acgtgttcaa agccctgtcg cgacagcatg gcggcggtcg
      901 atggtgacgg gcagaaggtg agcacgcact ggttatggat gaagcggttg tagctccaca
      961 tgggcctggt gaaggcggaa aagccgaaat gaccgcagta ggctgccagg ttcgtatggt
     1021 aggacgaaat gatgggcagg ttcaggaagc gcgcggcggc cagccctgcc gcgcccagca
     1081 cgacgggatc gacgagatgc acgatgtccg ggcgaaactc cttcaggcgg cggatgaaga
     1141 gcggtcgaaa aaagttgaat ttgagttcgg gatagaatgg aagtggaagg cctgccgtgc
     1201 cgacaatttc agcctcggcg tagtgttcca tgccgctctc tggcccaagc agcagcgcgc
     1261 gatgcccctg ttgttgcaaa tgttcaagca ggcgcgccag tgtgcgtgtg acgccgtcca
     1321 gctttggtag gaagttctcg gtgatgatgg caactcgcat ggcatttaac tccttttcac
     1381 tcattacaac cgtaccatgc aatctttagc gcgttattaa cagcctgtta gatcgggtaa
     1441 tcagttggct aagtaatggt taaatcgcgt ggcataggaa gggagtacca ttccttctga
     1501 acaggtatga gggaatcggg cataatcata gatagagagt gaagttttac gaacgcggta
     1561 cgttattacg taatagagtt tgctatcttg tgttgtgagg tgtgcgagcg tgaggggctg
     1621 tggtacggaa aagtgtcggc gctatactct taccaattat actgttctaa ttgtaagcta
     1681 tcaggaagca ggtacctacg attgaaatcc caatttatta aagcgaatga attgcaggaa
     1741 aacgcagatg tgccacgcca tatagccgag atgaatgcct gggcatccac tttttctccg
     1801 catgatagca gcgcaggtgg gtatgcttcg ccttcgattg tagaccagga tacggtagaa
     1861 cagcgcgcag ctcaagcggg gtcgtggacg caagtggctc aactgccgct atcacagcag
     1921 caacagaatc agcagtacca gcgccaggga gcagccgggg gtatcgtgtg ggacgatgcg
     1981 attgacacgg ctgtcatggc cattatgcaa ggcccggtct ctccgagcga atccggcata
     2041 acgcctcatg ccagattgta caatggcgtg tccaatactg tcaacgggcc acattcgcag
     2101 gcgttgcatc cgcctctaca gcagagctat ggccaacaag cgcagtataa tgccttcgct
     2161 gcggtgcagc cagccgggtt tgtgccgcaa gcgcaagcgc tatctgctcc cgcgcagggg
     2221 caggtgatgg gtatgccaac gatgatggct gcttttccga ccgcgcccgc gtaccagccc
     2281 tatcaaccac aacagcagta tcaacagtac cagcaaggta tggatgggcc gccgccctat
     2341 atgcagccgc cgctgccgcc attgccgccg ggaggcagac acaggaagaa acgccgcttc
     2401 cccatctggg cgcgcgcgct tgtcggtgtg ctactcttcc tgatgctgct cggcggcggc
     2461 ggatatgcct attatctgat caatttctcg tcggcggtcg ggaatgacac caatcaggcg
     2521 gcggcgcgct tgaaggggga tgacaacccc aatcagaact tgaacggcaa tattttgagc
     2581 ggtccgcgtg ttaatattct cctgttggga agcgatacgg atcaaaagtt cggcacgacg
     2641 gcgggcgggt caaccgtcta cctggcgcag accgacatta tcgtctcgat cgatcctacc
     2701 acgaagtcgg tcgtgatgct ttcgatccca cgcgacttct atatcaatat tcccggctac
     2761 ggcatgggta agctggatga agccttcgcg catggctaca gctacggtca gggcggtttc
     2821 ctcggcgggg tcgcgctgag ccgtctgacg atttaccagg acttcggcat ctcgatcaac
     2881 tattatgcct gggtgggcct ggatggtttc gtgaaggtga tcgataccgt tggcggcgtc
     2941 gatgtcgatg tgctgcatcc gattaccgat gatgcctacc ctgatgatgt tggaaacaag
     3001 accggcgata cgtatgccta caaacgcctg tacattccgc cggggccgca gcatctcagc
     3061 ggaggtgatg cactggatta tgtgcgttct cgccatgccg acctcgttgg ggactttggc
     3121 cggtcggcgc gacagcagca ggtgctgagc gcgctcaaga tcaagctggc taatcccaac
     3181 atcatcgggc agttgccgca acttgccagc gatctgaatg gctgggtcaa aaccgacatg
     3241 cagctgccgg atgttatcaa actaatgaat ttcgcgcgca cgcttgacgc gagtacagtg
     3301 caacgggttg tgttggagcc gccatattcc tcgtctacta cgctgccgag cggcgagtcg
     3361 attgtcgagc cgaattgctc ggcgatcgtg ccggagatca ccaaggtact ccagctcagc
     3421 actgcgcgct gcaatattta cgccagcagc aacacgacgc agatcgcctc ttcatcgcca
     3481 ggcgccaggc cggttgtcaa cggcagcgcc gatattcagc aaatcgccca ggcgagcagt
     3541 caactggcga gcctcaatcc gctcaacctg gttggcggga gcagtgacct gttcggcatt
     3601 catagtatgc tcgatctgct cttcaccgtc gtctttgaat cgccgcaggc gatgcaagtt
     3661 tgaagcagga cctatcacga tgagaagcac gagaaaacaa aagccctggt tctccttcgc
     3721 cctgctattg ctgctctctt tgagtgtaca gggatgcctg ggctttggca gcagcacgcc
     3781 accaaacagc acgactatca cccagcacgg taacggtgga cagcaggtca cagtagcgca
     3841 aaatctgttc aagggcaaaa tatacctgac gatcgatcac aacctgtggg tgtttaccgg
     3901 gaataacaaa tacaccgagt tgcttagaag cggcaatgtt tatgacccgg ctgtctcgcc
     3961 ggatggtaaa tggattgcct ttgtgtatcg atttaaggat tactccgact tgatggtgat
     4021 cccatctggc ggagggccag agcgcaaact gctggatggg aatgggcatt tctatattga
     4081 tagcggcttc cccaaggaca cctacttctg gtttgtgcaa ccatcctggt ctccggatgg
     4141 tacgcatatt ctcttcttga gtgatctaca aaagaattat gtctgggcgg gtttgaacaa
     4201 tctcttcaag aacagctatt tcctggactt gcaggttttt tcgatcccgt ttaatgatcc
     4261 gaccgctact ccccaggtgc tggcttatgc caactttggc gatggcggca accgcgatgc
     4321 tgcgtaccag cctgaacatt ccagcacgag ccagatcatc tacacccact acgcctatga
     4381 cgcgcaaacc ggcacacagc aggtgattca gatttacatg gcggatgcca acgccatcgc
     4441 cgatcatcct ggcacctatt cgccggtcta cgattcgggt gtggcgctga cgccaggcag
     4501 cgcggagaat atccagccag cgttttcgcc caatggcaac ttcatcgctt atatacggcg
     4561 cgagtcggct acgtcgatgg ggctgtatgt gatgtccacg ccggtaggag tgactgccga
     4621 tccgaacagc cagaccagcc aggcgcaggc attgatccca tataagacct cgtcgcatct
     4681 atggagcagc cagtatgtga gccagcccat ctggtcgccc gatggcaaac aaatcgcctt
     4741 cctggaatat accaacaacg agttcgatct gtggctagcc aatgtcagct ataacgcgca
     4801 gacggggcta ttcaagttgc agggcagccc catgcaaata actaccggcg gcgtcgatgg
     4861 cgaatcgcga cccgtctgga cgacgtagac gcgccaaacg tgttcatgat atactatgca
     4921 gacgttctta ggcgttaatg attgtagggg cgcaattcat tgcgcccggt agaagcgtgt
     4981 aacagagtgc gaaggcgcaa tgaattgcgc ccctacaggc gggtcatctc ccaggttttt
     5041 gaggtaggac acattgacac aatttgaagg cacgttgatt gccaagcggt acgagattcg
     5101 cgagcatatc gccaccggcg gtatggcgag cgtcttcaag acctgggatc atcgagttga
     5161 acgactggtt gccatcaagg tgctgcggtc gctggacaag aacgatcagc gcgcggttga
     5221 gcggttccgc cgcgaggcgc gcgcggcggc ggccctggcg catcccaatg ccgtgaccat
     5281 ttatgacttc gtcgaggagt tcgggcaata tttcctggtg atggagtata ttcacggccc
     5341 gacactcaag cagttgatcg ggcagcgccg ccacctgcac gcctatgaga cgctggagat
     5401 cgccgcgcag gtctgcgccg tgcttcaggt agcccatgcg cgcggtttca tccaccgcga
     5461 catcaagccg cagaacatca tgctggcctc cagtggggcg tccagtccgt tgatcgccag
     5521 cgccatcgcg ccatcgacca gtttgctcgt caagctgacc gatttcggaa ttgtgcgcgt
     5581 ggcggaagat gccggtctga ccaacagcgg gattgtgctg ggaaccgccg attatctttc
     5641 gccggagcag gcgcgcggcg agaagctcgg accgtcctcc gacctgtatt cgctgggcgt
     5701 ggtaatgttc gaaatgatta ccggtcgtcc gccctttatc ggcccgactg ccgtctccat
     5761 cgcgatgcaa cacgcatcga ccgatccgcc tcccctgcgc cagttcacca gcaatgttcc
     5821 cccggcactg gaacagatcg tcagtaaggc gctggcgaag gagccggagg gaaggtttct
     5881 ttcagcggca gagatgcagt acgcactgcg ccagtgcgcg cgcgaactgc ggggacacgc
     5941 caactatatt accgcgccga tttccaaccc ggctcccagc gctggcggtt acgcgccgac
     6001 gcacccgccg ctctatcgat cataacaacg actatcgctc aggaagtaag acatggaaca
     6061 cacgcagatc agcacacctc acgcacccgc cgctatcggg ccgtatagcc aggccattcg
     6121 ctacggccag ttcatctacg catcgggcca ggtcgcgctc gatcccgcca gcggtgcact
     6181 ggttggcaca gatgtcgagg cgcagactca tcgcgttttc cagaatatcc aggcggtact
     6241 ggcaaccgcc aataccaccc tggcgcacgt ggtgaaaacg accgtctttc taacacgcat
     6301 gagcgatttt caggacatga acgccgtcta cgccagctat tttgccgcga ccggcgctcc
     6361 cgcccctgct cgctcaacgg tcgcggtagc agagttgccc cgcaatgccc tggtcgagat
     6421 cgagtgtgtg gcctacgtag aagcataggg ggagaagatg gccaataaca cgattatctg
     6481 gcacgccacg cttgatcgcc tgcaagatgc gcagatggat gccgtctgcc gggcgtggct
     6541 gcaatccgcc aaccttgcca gcgcgccgaa ctttggagca gacgatatcg atgccgaaga
     6601 cctggtaggg caggaagagc cgctctattt taccctgcac gtgccaagtc ccctggcgcg
     6661 cgatgtgatc aatacacgct ggcggcgcag catcgaagac atcctgaccg atctgaccgg
     6721 tcagccggtg acgatcgtgg tgagcgacca tttctacgag gatgggctgc cctacggact
     6781 gcaagccatc cccgattccg ccggaggtcg ctatattcct ggcggctact actcgcgcga
     6841 gagcaatccc tacccgccca cgccgcgcta cgcgcagggc aacgactacg cctcgtatga
     6901 tttcccggtg cgcccgtcgc aggagctgcc cgaagcagga agcatgatgg gtggcgcgaa
     6961 tggagggagt gcaagtcgct atatacccga accagccgca ccctactacg cgcagaccga
     7021 tgactggaac aacaacgagc agcgcgctac gatcttgatg cagaaccgcc tgaacccgcg
     7081 ctacacgttc gatgccttta ttgtcggcaa cagcaatcgc ctggctcatg cggcctcgct
     7141 ggccgtagct gaggcgcccg
//
LOCUS       DASZAK010000039         3767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_675225, whole genome shotgun sequence.
ACCESSION   DASZAK010000039 DASZAK010000000
VERSION     DASZAK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675225"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..939
                     /gene="iolB"
                     /locus_tag="VGT44_00665"
     CDS             34..939
                     /gene="iolB"
                     /locus_tag="VGT44_00665"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEV2579335.1"
                     /translation="MRKYDQSNLIVHPGNSTDPDVIVEVTPELAGWDYVHFQLRRLPA
                     QRSWSFTTAEHELAIVPMSGRVRVESNRGQWSHIGERDSVFHGLPYALYLPRRTSLTV
                     YAETACEYAVALAPTDQDHPPRLVTPSDIEIEIRGGDNATRQINNIIPPGFPCHRLIV
                     VEVYTPGGNWSSYPPHKHDVHRTDQAGKVLEADLEEVYFYKLDRPEGFAFQRIYTAPE
                     SPLQRAGFPIDAVLLARDNDAVLVPEGYHPVTSPPGYTTYYLNVLAGSAQSLANSEDP
                     RYTWVKESYRSQDPRVPIYDVTPNV"
     gene            958..1989
                     /gene="iolC"
                     /locus_tag="VGT44_00670"
     CDS             958..1989
                     /gene="iolC"
                     /locus_tag="VGT44_00670"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEV2579336.1"
                     /translation="MDAATGSYDVIAMGRSSIDLYSNDIGAPFVDITSFAAYVGGCPT
                     NISVGTRRLGLRSALLTAVGDDQVGEFILHFLHNEGVETRFIPHKQGRRSSAVVLGIE
                     PPDRFPLTYYRDNCADIELTIDDVLAAPIAGSHALLISGTGLSKEPSRSATLFAAETA
                     HRGGGQVMLDIDFRPDQWHDPRAFGVTLRSALRLVDVVVGTEDEINAVMLTDPSQISL
                     THSQVSDARVSGDINAAIRALLDLGPQVLAHKRGAAGSTVHLVLNGDVVTQIEAPGFP
                     VVVENILGAGDAFASGFLYGLVNGWDWYRTARLANACGAILVTKHGCANFMPTYEEVM
                     TFIQNHGGF"
     gene            2024..>3767
                     /gene="iolD"
                     /locus_tag="VGT44_00675"
     CDS             2024..>3767
                     /gene="iolD"
                     /locus_tag="VGT44_00675"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEV2579337.1"
                     /translation="MDKQATQRMTMAQAVIAFLKNQYVERDGVELPFFAGCFGIFGHG
                     NIAGIGQALQQMSEFRYYQARNEQAMVHIATAYAKTRNRMQTLACTSSIGPGATNMVT
                     GAATATINRLPVLLLPGDIFARRNVAPVLQQLESEHTQDISVNDCFKPVSRYWDRITR
                     PEQVLTALPEAMRVLTSPVETGAVTLALPQDVQAEAYVYPEAFFHKRVWHIPRNRADR
                     RALEQAAAWIRASQRPLIVAGGGVIYSEATGTLQRFVDQTGIPVGETMAGKGSLRYDH
                     VQSLGAIGATGTFAANRVAKEADLVIGIGTRYSDFTTASKTAFQHPDVRFININVAGF
                     DASKHLALPLTGDAKVTLEELLALLDGYHVDEGYRATVEQLHVAWDAEVERIYAIRNE
                     PLPSQGELIGAVNELSAPDAIMVCAAGSLPGDLHKLWRTRHPKNYHMEYGYSCMGYEI
                     AGGLGVKMAAPDRDVYVLVGDGSYLMMNSEIVTSIQENYKLIIVLLDNHGFKSIGSLS
                     RSLGEDGFGTRYVYPRNGVLPGDEAGEDVQILPVDLAANARSLGAHVIECRTYADFVA
                     ALEAARATDRTSVIY"
BASE COUNT          798 a         1045 c         1094 g          830 t
ORIGIN      
        1 aagatattga gctttatatt tgggagtaga tctatgcgta aatacgacca gagtaacttg
       61 attgtccatc ccggcaactc caccgatcca gatgtaatcg tcgaggtaac gcctgaactg
      121 gcaggctggg attacgttca tttccagttg cgccgcctgc ccgcgcagcg ttcctggtca
      181 tttaccactg ctgaacacga gctagcgatt gtgccaatga gcggtcgtgt acgtgttgag
      241 tcgaaccggg gccaatggtc gcatattggt gagcgagaca gtgtcttcca tggtctgccg
      301 tacgcgctct atctaccccg gcgtacctcg ttgaccgtgt atgctgaaac cgcgtgcgag
      361 tatgccgtcg ctctagcgcc gaccgaccag gatcatccgc ctcgcctcgt cacaccgtct
      421 gacatcgaga tcgagatccg ggggggcgat aatgctacgc ggcagattaa caacatcatc
      481 ccaccaggat tcccatgtca caggctcatt gttgtggagg tctacacgcc gggcgggaac
      541 tggtcgagct atccaccaca caagcacgat gttcatagaa ccgaccaggc aggcaaagtg
      601 ctggaagccg atcttgaaga ggtctacttc tataaattgg atcgcccgga gggatttgcc
      661 tttcaacgta tttacaccgc tccagagtcc ccgctgcagc gtgcaggctt tcctatcgat
      721 gctgtgttgt tggcacgcga taacgatgcc gtgctcgtac cggaggggta tcacccggtg
      781 accagtccac ctggctacac cacctactat ctcaatgttc tggcaggcag cgctcaatcg
      841 ctcgccaaca gcgaggaccc gcgctacacc tgggtcaaag agagctacag gtcacaagat
      901 ccgcgggtac ccatctacga tgtaacaccc aatgtatgag aggaacagga gccagctatg
      961 gatgcagcca ccggatcata cgacgtgatt gctatgggtc gatcttcgat cgatctctat
     1021 tccaacgata tcggtgcccc gttcgtggac atcaccagct ttgctgccta tgttggcggc
     1081 tgcccgacga atatcagcgt cggtacgcgg cgtttgggcc tgcggtcggc gctattgaca
     1141 gcggtcggcg atgaccaggt gggcgaattc atcctgcatt ttctgcacaa tgaaggggtg
     1201 gaaacacgtt tcatcccaca caagcaggga cgccgttcca gcgcggtcgt gctggggatc
     1261 gaaccgcctg accgctttcc tctgacgtat taccgtgaca actgcgccga tatcgaacta
     1321 accattgacg acgtgctggc ggcgccaatc gccgggagcc acgctctgct gatttccggt
     1381 accggtttga gcaaggagcc aagtcgcagc gctacactct tcgctgctga gacggcgcat
     1441 cgcgggggcg ggcaagtgat gctggatatt gacttccggc ccgaccagtg gcacgaccct
     1501 cgcgctttcg gcgtcacact ccgctcggcg ctccgccttg tcgatgttgt ggtaggcacg
     1561 gaagacgaaa tcaatgctgt catgctgacc gacccgagcc agatcagttt gactcattct
     1621 caagtctcgg acgcgcgcgt aagcggcgat atcaacgccg cgatccgggc gctgcttgac
     1681 ctgggaccgc aagtgctcgc tcacaaacgc ggcgcggcgg gctcgacggt tcatcttgta
     1741 ttaaacggcg atgttgtaac gcaaatcgag gcgccagggt tccccgtggt ggtggaaaat
     1801 atcctgggtg ctggtgacgc atttgcctcc ggtttcctgt acggcctggt caatggatgg
     1861 gattggtaca ggactgcacg cctggcgaat gcctgtggcg caatcctggt cacgaagcat
     1921 ggctgcgcaa acttcatgcc aacctatgaa gaggtcatga cctttattca aaaccatggt
     1981 gggttctagg gccacgatta tctagaatta tgggaggatg aacatggata agcaagcaac
     2041 gcaacgtatg acgatggccc aggccgtcat cgcatttctc aaaaaccagt acgtcgagcg
     2101 ggatggggta gagcttccct ttttcgccgg gtgctttggt atctttggtc atggcaatat
     2161 cgcgggtatc gggcaagccc tgcaacagat gtccgaattt cgctactacc aggctcgtaa
     2221 cgagcaggcc atggtgcata ttgccacagc atatgctaaa accagaaacc ggatgcagac
     2281 gcttgcgtgc acttcttcta ttggccctgg tgcaaccaac atggtgaccg gcgccgctac
     2341 cgctacgatc aaccgcctgc ccgtgctctt gctgccgggg gatatttttg cgcgccgcaa
     2401 tgttgcgcct gtcctgcagc agcttgagtc cgagcacacg caggatatct cggtcaatga
     2461 ttgtttcaaa ccggtcagcc gttactggga tcgcattacg cgccctgagc aggtattgac
     2521 cgcactgccc gaggctatgc gcgtattgac aagcccggtg gaaactggcg ctgttacact
     2581 cgcactccct caggatgtac aggcagaggc gtatgtttat cccgaagcgt ttttccacaa
     2641 gcgcgtgtgg catatcccgc gtaatcgtgc ggaccgcaga gcgctggaac aggcggcagc
     2701 atggattcgc gccagtcaac gaccgttaat cgttgcgggt ggaggtgtaa tttactcgga
     2761 ggcgaccggt accttgcagc gcttcgttga ccagacgggc attcctgttg gcgagacgat
     2821 ggccggaaag gggagcctgc gctatgacca cgtgcagagc cttggagcaa tcggcgccac
     2881 cggtacgttc gcggctaacc gcgttgcgaa agaagcggac ctggtcatcg gcatcggcac
     2941 gcgctacagt gacttcacaa ccgcgtcaaa gactgcattt cagcatcccg atgtgcgctt
     3001 catcaatata aatgtagccg ggttcgatgc ttctaagcac ctggccctgc cgctcacggg
     3061 tgatgcgaag gtgacgctag aagagttgct tgcgctgctc gacggctatc atgtcgatga
     3121 aggttatcgt gccacggtcg agcaactgca tgttgcatgg gacgctgaag tcgaacgcat
     3181 ctatgcaatc cgcaatgagc cactgcccag ccagggcgag ttgatcggcg cagtcaacga
     3241 gttgagcgct cctgatgcca tcatggtgtg tgccgcaggc agtttgcctg gcgacctgca
     3301 taagctctgg cgcactcgcc atcccaagaa ctaccacatg gaatatggat atagctgcat
     3361 gggttatgag atcgccggtg gattgggggt taagatggca gcgcccgacc gcgatgtgta
     3421 tgtgttggtt ggcgacggct catacctgat gatgaatagc gagattgtga catcgatcca
     3481 ggaaaactac aagctgatca ttgtgttgct cgacaatcat ggtttcaaga gcataggcag
     3541 tctcagtcgc tcactcggcg aggatggctt tggcacccgc tatgtctatc cacgcaatgg
     3601 ggtattgccc ggcgacgagg caggcgagga tgtgcagatt ttgcctgttg acctggcagc
     3661 gaacgcacgt agtctgggtg cgcacgtgat cgaatgccgg acctatgctg atttcgttgc
     3721 cgcgctcgag gccgcgcgtg caaccgatcg cacgtcggtg atctaca
//
LOCUS       DASZAK010000040         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_462355, whole genome shotgun sequence.
ACCESSION   DASZAK010000040 DASZAK010000000
VERSION     DASZAK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462355"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..375)
                     /locus_tag="VGT44_00680"
     CDS             complement(184..375)
                     /locus_tag="VGT44_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579338.1"
                     /translation="MRAIQPYLSGQAGDYVLRAVSQQFLSEHWQDGQCRVVPQVPGRA
                     SSSKPYPLSRCLSRRRRRR"
     gene            complement(435..511)
                     /locus_tag="VGT44_00685"
     tRNA            complement(435..511)
                     /locus_tag="VGT44_00685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(475..477),aa:Pro,seq:cgg)
     gene            941..1144
                     /gene="rpmI"
                     /locus_tag="VGT44_00690"
     CDS             941..1144
                     /gene="rpmI"
                     /locus_tag="VGT44_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV2579339.1"
                     /translation="MKQKLKTHTAMAKRVRVTGSGKFVRRKVAISHLRRNKSPRLIRS
                     ADKTVRLATADTRRLKRLLPYSF"
     gene            1175..1531
                     /gene="rplT"
                     /locus_tag="VGT44_00695"
     CDS             1175..1531
                     /gene="rplT"
                     /locus_tag="VGT44_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV2579340.1"
                     /translation="MPRVKRGVAAHKRHKRLLKMAEGHRGTRNNLYRPAHESVMHAMA
                     YMYRDRRNRKRDMRRLWITRINAAARMHGVSYSKLMHAIHTANIEVDRKILAEMAVND
                     EAAFTALVQAALATIQ"
     gene            1598..2437
                     /locus_tag="VGT44_00700"
     CDS             1598..2437
                     /locus_tag="VGT44_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV2579341.1"
                     /translation="MTLITSPTNPRVREARDLLTGKGRKQHGLFLMEGPHLLETLLDV
                     GIMPVEIFYLPALLEHTVQGGALFRRLLSTPAAERLVEVSERVMQALSDTEAPQGVVS
                     VLPLAAFAPERARARRPQAQRPALLILDALADPGNMGTILRTALAADVEAVLLTPNCV
                     DCFSPKVVRSASGAHVALPIAINQSWAAIERQVEAHCQGQSRVFLAEAGREPSYFEQD
                     FRAPFALIIGNEAHGPSQQARSLSTAALSIPLANNVESLNAAMAAGIILYEAVRQRYH
                     QTE"
BASE COUNT          614 a          767 c          738 g          546 t
ORIGIN      
        1 aaagtagcac gatgagacga cgttcaggaa tgactgaaag aggttggaat gaagaattca
       61 acacaagtac gggcatgaat gacttccctt cctctctgtc aaggccgata ccgattgcag
      121 acagcgagga agaagcgaac aaagagcgaa aggacgtggg gagtagccca ttcctgtttg
      181 cttctaccgc cgcctgcgac gacgactaag acaacgactg agtggatagg gtttgctact
      241 acttgcgcgt ccaggtacct gcggcacaac gcggcattgg ccgtcctgcc agtgttcgga
      301 taagaactgc tggctgacgg cgcgcaacac atagtctccg gcctgaccgg aaaggtatgg
      361 ttggattgcc cgcattttga actcctgttg ttacctgttg atgtgacacc tgttgtgaaa
      421 cgagtgtgcc atcctggtcg gggtgagagg acttgaacct ccgacctcag cgtcccgaac
      481 gctgcgcgct agccgactgc gctacacccc gattgaacta accacgcaag tgatctatcg
      541 gccctaccca ataaaccatc gagtgcgcga tcaatacgat aaagttgctt gcaaagcccc
      601 ttcactcgca tttcaacacg cctcccctgc tacaagcata gtatagcata caggtataga
      661 gttggcaata gttgcacaga tttaaagatt tggagcagcc cttgtcagga tgaggcaact
      721 atgctataat cagcgcgtga cgcggggtat gctcccgaaa acaggcttct ctgcgccttc
      781 gacggagcaa caacggtgga ttgaagcgca atctcagtca tcgccttcat cgtaatacca
      841 cctctctgtt gtgccgccca ggtggatgtc accgggcggc ttcacagcgc aaaatgatcg
      901 ttctatactt gatattgccc cgccagaaat ggaaagaaac atgaagcaga aactgaaaac
      961 gcacaccgct atggcaaagc gggtcagagt taccggctct ggcaagtttg tgcgccgcaa
     1021 agtggctatc agccacctgc gccgcaacaa atcgccccgg ctcattcgct cggcagataa
     1081 gaccgttcgt ctggcaactg ccgatactcg tcgtttgaaa cgcctgctcc cttactcgtt
     1141 ttaagcacag acagcgaatg gaggaaaaga aacaatgccg agggtaaaac gtggcgtcgc
     1201 cgcccacaaa agacacaaga ggctgctcaa gatggccgag ggtcatcgtg gaacacgcaa
     1261 taacctgtat cgtcccgcgc atgaatccgt catgcacgca atggcctaca tgtaccgcga
     1321 ccggcgcaat cgcaagcgcg acatgcgccg cctgtggatc actcgcatca atgcagccgc
     1381 gcgtatgcat ggtgtcagtt atagcaagct catgcatgcc attcacaccg ccaacatcga
     1441 ggtagatcgc aagatcctcg ccgaaatggc tgttaacgac gaggcagcct tcactgcgct
     1501 ggttcaagca gcgctggcga cgatccaata atttaatcat attcaaccac caacctgccc
     1561 gtacgggcag gttggtggtt gaatatatgc tgtgtttatg acactcatca ccagtcccac
     1621 caatccgcgc gtgcgcgagg cgcgcgacct gctgaccggc aagggccgca agcagcatgg
     1681 cctctttttg atggaagggc cacacttgct tgagacactg ctggatgttg gcatcatgcc
     1741 cgtggaaatc ttctacctgc ccgcgctgct agagcacact gttcaggggg gggcgctctt
     1801 tcgtcgtctg ctctcgactc cagctgccga acgcctggtg gaggtcagcg agcgcgtcat
     1861 gcaggcgctg agtgacacgg aggcaccgca aggagtggtc agtgtgctgc ccctggccgc
     1921 gtttgcccca gaaagggcgc gcgcgcgacg accgcaggcg cagcgacccg cgcttctgat
     1981 cctggacgcc ctggccgatc ccggcaacat gggaactatc ctgcgcaccg cgctggccgc
     2041 cgatgtcgaa gcagtacttt tgacgcccaa ctgcgtcgat tgttttagcc ctaaagtcgt
     2101 gcgctcagcc tccggcgcgc acgtcgcact gccaatcgcc atcaatcagt cctgggccgc
     2161 tatcgagcgc caggttgagg cgcattgcca gggccagtcg cgtgttttcc tggcggaggc
     2221 aggccgcgaa ccgagctatt tcgagcaaga ttttcgcgcg cccttcgcgc tgattattgg
     2281 aaacgaagcg cacggaccgt cccaacaggc gcgttcgctc tcgaccgctg ccctgtctat
     2341 tccactggcg aataacgtgg aatcgttgaa cgcggcaatg gcagcgggca tcattttgta
     2401 cgaggcggtt agacagagat atcatcagac agaataaagc agcctcaccg atgaggctgc
     2461 tttattctgt tctctattta acgcgccgat ttaccagcgg ctgcccattt cacgacagat
     2521 ggaatctgtg tgggcaagct ctaccgagcc gccgcagctt tcgcaggagg tgggcgcgga
     2581 accggcgtga accatgccac atgtctcgca gatccagcga taggagagca aggtgggtcg
     2641 tcggctgagt agtttcattg ctgcc
//
LOCUS       DASZAK010000041         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_249454, whole genome shotgun sequence.
ACCESSION   DASZAK010000041 DASZAK010000000
VERSION     DASZAK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249454"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..533
                     /locus_tag="VGT44_00705"
     CDS             57..533
                     /locus_tag="VGT44_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579342.1"
                     /translation="MDLWTSPSGLSQFFSDPQVQQGAGLIFTQRDPVLWEPAEGFFTY
                     HIPAPTGHNDRFFGLIRGPVASREQARAIMNQVTGQGIHKARAAGHLSHEVYFRLTQP
                     GTPESLELFAIDAWSDLDGMNRYYDDPDFVHAFEGLFTAQPTTLALKHPAGQWVEW"
     gene            complement(721..>2057)
                     /locus_tag="VGT44_00710"
     CDS             complement(721..>2057)
                     /locus_tag="VGT44_00710"
                     /inference="COORDINATES: protein motif:HMM:NF039390.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579343.1"
                     /translation="DKTLVIHTDKSGEITKKSLDAARKLAREVDSPDCPTNAIVSVLM
                     LREGWDVQNVTVVVGLRPYTAAANILPEQAIGRGLRLMFRGQSYAERVDIIGNKKFLE
                     FVDDLERLEELQLDTFEIGKDRLKIMAIAPAPEKAAYDLALPRLTPILARKKTLAEEI
                     EALDITRFRFSPDPLPRKPGDVDIKKFQYEAFDIITREKLFEREYAIQDAQRPDEIVS
                     YYARIIASSLKLPGQFYALAPKVWSFFERRAFGGTVDMYSPTIIKAMNHRVAGYVVLD
                     VFARALRDKIIEQLEPAIEGENRHLSYMEPFAYSRTVHEARKCILNYVPCDNDFEFEF
                     ARFLDNAKDVEAFSKLPQQFGFSIEYTDNNANLRYYYPDFVVKTGTGEMWLVETKGQE
                     SVEVAFKDRAARLWCENASLLTGERWSYMKVPQKEYNRLQPVEFCDLLAFVG"
BASE COUNT          412 a          607 c          574 g          464 t
ORIGIN      
        1 agttgaggca catggtctag gtccccgcga atggagggtt tggcgaactc gtcatcatgg
       61 atctgtggac aagcccgagc gggctgagcc agttcttttc cgatccgcag gtgcagcagg
      121 gagcagggct gattttcaca caacgcgacc ctgtgctctg ggagcctgcg gaagggttct
      181 tcacctacca tatcccagcg cctaccggac ataacgaccg tttcttcgga ctgattcgtg
      241 gacccgttgc ctcccgcgag caggcccgcg ccattatgaa tcaggtgacg ggtcagggta
      301 tccacaaggc cagggcagcc gggcatctgt cccacgaggt ctacttccga ctcactcaac
      361 cgggaacgcc cgaatccctc gaactctttg caatcgatgc atggagcgac cttgatggta
      421 tgaatcgcta ctacgacgat ccagatttcg tccatgcctt tgaaggactc ttcaccgcac
      481 aacctacaac tttggcactg aagcacccgg ctggccaatg ggttgagtgg taaagtacca
      541 gaacgttctg cgttgctgta cagtttctgt catgggtgaa tttcgcaaaa ataaaaacgt
      601 ctcaggcccc ttcaagactg gcctgggacg tttctagctg tctctcctgc ggctcctcaa
      661 gcgagtttac gtctgcagat gtttttggtt atttacatat gaaataatat acagcattat
      721 tcagcccaca aacgccagca aatcacaaaa ctccaccggc tgcagccgat tatactcctt
      781 ctgcggcacc ttcatatagc tccatcgttc gcccgtcagc agtgaggcgt tctcgcacca
      841 aaggcgcgcg gctctgtcct tgaacgccac ctcgaccgac tcctggcctt tcgtttcgac
      901 cagccacatt tcgccggttc cggtcttcac cacaaagtcg ggatagtagt agcgcagatt
      961 ggcgttgttg tcggtgtact ctatagagaa accgaattgc tgcggcaact tggaaaaggc
     1021 ttccacgtcc ttcgcgttgt cgaggaagcg cgcgaactcg aattcaaagt cgttgtcgca
     1081 gggaacgtag ttgaggatgc atttcctggc ctcgtggacg gtcctggaat aggcgaaggg
     1141 ttccatgtag gacaggtgcc ggttctcgcc ctcgatggcc ggctccaact gctcgatgat
     1201 cttgtctcgc aaggccctgg cgaatacgtc aagcacgaca taaccggcca cgcggtgatt
     1261 catggccttg atgatcgtcg ggctgtacat atcgaccgtt ccgccaaaag cgcggcgctc
     1321 gaagaagctc cagaccttgg gggccagcgc gtagaactgg ccgggaagct tcaagctgct
     1381 ggcgatgatg cgcgcgtagt aactcacgat ctcatcgggt cgctgggcgt cctggatggc
     1441 gtactcgcgc tcaaagagct tctccctcgt gatgatatcg aacgcctcgt actggaattt
     1501 cttgatatcg acgtcgcctg gcttgcgcgg caggggatcg ggactgaagc ggaagcgcgt
     1561 gatgtccagc gcttcgatct cctcggcgag cgttttcttg cgagccagga tgggcgtgag
     1621 ccggggcagc gccaggtcat aggccgcctt ctccggcgct ggcgcaatcg ccatgatctt
     1681 gagcctatct ttgccgatct caaacgtatc aagctgcagc tcttccagcc gctccaggtc
     1741 atcaacgaac tccaggaatt tcttgttgcc gatgatgtcc acccgctcgg cgtagctctg
     1801 cccccggaac atcaggcgca ggccgcgccc gatggcctgc tccggcaaga tgttggcggc
     1861 ggcggtatac ggacgcagcc cgaccaccac cgtcacgttt tgcacatccc agccctcgcg
     1921 cagcatcagc acgctgacaa tcgcgttggt cgggcaatca gggctatcaa cctcgcgggc
     1981 gagcttgcgg gcggcgtcaa ggcttttctt cgtaatttcg ccggacttat cggtgtggat
     2041 caccagcgtc ttgtcgg
//
LOCUS       DASZAK010000042         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_213925, whole genome shotgun sequence.
ACCESSION   DASZAK010000042 DASZAK010000000
VERSION     DASZAK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213925"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..727)
                     /locus_tag="VGT44_00715"
     CDS             complement(38..727)
                     /locus_tag="VGT44_00715"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2579344.1"
                     /translation="MSQQPIDQAVAYLVIKQGRQAGKRLELWKDCTTIGRSTNCDIFL
                     EDLAVHRKQASIYFTERGYVLRDEHGNGDSFVNGQQIWECILKDGDTLSFGHTQLVFN
                     GHEGTRPFQLASSRNRESHLVKNPDPTASAIAKLDLASARGAMRSFEMMPEMTIGRSL
                     ECDIFLEDLAVSRLHATIREIGDGGYEIEDHRSATGTYVNGMRITRYRLLEGDIVQIG
                     TSKMTFKRSRG"
     gene            912..>2644
                     /locus_tag="VGT44_00720"
     CDS             912..>2644
                     /locus_tag="VGT44_00720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV2579345.1"
                     /translation="MAMQVGQEITVEFDVQASMRDGAILRANIYRPAGEGKWPVLLTR
                     LPYGKDLPGGTSVMDPAQVARRGYVVIVQDTRGRFTSEGEWVPFINEGLDGVDTIEWA
                     AQMPYSNGSVGMYGISYFGFTQWVSAVYNPPALKAMVPFQTWNDPLNGVIFRGGALEL
                     GSSGGWQLMMGMDVLIRRHRGDPQALGRAIYLLAKEIDALGKMGYQSLPLREFGPLKR
                     HEIAPTFFENFDHPMDRAHELVEPMTILGKHEQVTVPTLNVGGWYDIFLQDTISNYTT
                     MREHGATPEARQSKLLIGPWSHGGVTNPIGELNFGFGASAAFIDLQMDFGSMQVRWFD
                     HWLKGIDTGMLKEAPIKLFVMGANAWRDEQEWPLTRAVNTRYYLHSLGKANSLHGNGY
                     LLASAPERDEPTSDSFDYDPGDPVMTRGGALLMSPEYPSGPFDQRQTEGRNDVLVYTS
                     AELTEDVEVTGPITVHLWAVSSAPDTDFVARLVDVSPDGYAQNLTDGIIRARYRNFAR
                     GEAPSLIEPGTAYEYDIDLWATSNVFKAGHRIRVDITSSNFPRWDRNPNSGHAFGADS
                     ETVVAHQTILH"
BASE COUNT          504 a          769 c          792 g          579 t
ORIGIN      
        1 gccatgtgtc gcgctcgcca tgtaacgtgg aggaccatca cccgcgcgag cgtttgaagg
       61 tcatcttgct cgtgccaatc tgcacgatat cgccctcgag caggcggtag cgcgtgatac
      121 gcattccatt gacataggtg ccggtggccg aacgatggtc ttcgatttca taccccccgt
      181 cgccgatttc ccggatggtg gcgtgcaggc ggctgacggc caggtcttcc aggaagatat
      241 cgcattccag gctgcgcccg atggtcattt ctggcatcat ctcgaaactg cgcatcgcgc
      301 cgcgcgcgct cgccaggtcg agcttcgcga tggcactggc cgttgggtca gggttcttga
      361 cgaggtgcga ctcgcggttg cgggaggaag cgagctgaaa tggacgtgtg ccttcgtgtc
      421 cgttgaagac caattgcgta tggccgaagc tcaacgtatc gccgtctttc aagatgcatt
      481 cccatatttg ttgcccattg acaaagctgt cgccgttgcc atgctcgtcg cgcaagacgt
      541 atccgcgctc ggtgaaatag atgctggcct gtttgcgatg cacggccagg tcttcgagga
      601 aaatatcgca attggtgctg cggccaatcg tagtgcaatc cttccagagt tccaaccgtt
      661 tacccgcctg gcgtccctgt ttgataacca ggtaggctac tgcttgatcg attggctgtt
      721 gcgacatctc tgtactcact tctagtctgc gtcgttgcgc gttcccttgc cactggtcaa
      781 atcataccat gtcgttctct ttcagacaag atgggtttat tggccatctc gtcgcgcgcg
      841 ttgtatgatg ggacaggtct tcaatagcca ggaacgttat tcctggaaat gcatagaagg
      901 agttccattg tatggcaatg caagtcggcc aggagatcac ggttgaattt gatgttcagg
      961 ccagtatgcg cgatggcgct atcctgcgcg ccaatatcta ccgtcctgcc ggtgagggca
     1021 aatggccggt gctgctgacg cgcctgcctt acggtaaaga cctgcccggc gggacttcgg
     1081 tcatggaccc cgcgcaggtc gcgaggcgcg gctacgtggt gattgtgcag gacacacgcg
     1141 gtcgctttac ctccgagggg gagtgggtgc cgttcataaa cgaggggttg gatggtgtcg
     1201 ataccatcga atgggctgcg cagatgcctt acagcaatgg cagcgtgggc atgtacggca
     1261 tctcctattt tggctttacg caatgggtgt ccgccgtgta taatccgccc gccttgaaag
     1321 cgatggtccc gtttcagacg tggaacgatc ccctgaacgg tgtaatcttt cgcgggggcg
     1381 cgcttgagtt gggcagttcg ggcggctggc agttgatgat ggggatggac gtcttgatcc
     1441 ggcgacatcg cggcgatccg caggcgctgg gccgcgctat ctacctgctg gccaaggaga
     1501 tcgacgcgct tggcaagatg ggctatcaat ccctgcccct gcgcgaattt gggccgctca
     1561 aacgccatga gattgccccc accttcttcg agaacttcga tcatccgatg gatcgcgcgc
     1621 acgaactggt tgagccgatg acgattctcg gcaagcacga gcaggtcacg gtaccaaccc
     1681 tcaacgtggg cggctggtac gatatcttct tgcaggatac tatctccaac tacacaacga
     1741 tgcgcgagca tggcgcgacg ccggaagcgc ggcagagcaa gctattgatc ggcccctggt
     1801 cgcatggcgg cgtaaccaat cccatcggtg agctgaactt cggtttcggc gcgtctgctg
     1861 cctttatcga cctgcaaatg gattttggca gcatgcaggt gcgctggttc gatcactggc
     1921 tcaaaggtat cgatacgggg atgctcaagg aagcgcccat caagctcttc gtcatgggcg
     1981 cgaatgcctg gcgcgacgag caggaatggc cgctgacccg cgctgtgaac acgcgctact
     2041 acctgcatag cctcggcaag gccaactcgc tgcacggcaa cggctatctg ctggccagcg
     2101 cgcccgaacg tgacgaaccc acctctgact cgtttgacta tgaccctggc gatccggtga
     2161 tgacgcgcgg cggtgcgctg ctcatgtctc cggaataccc ctcgggcccc ttcgaccagc
     2221 gccagaccga gggccgcaat gacgtgctag tctacacgtc tgccgaactc accgaggacg
     2281 tggaagtcac cggtccgatt acggtgcatc tgtgggccgt ttcgagcgcg cccgataccg
     2341 attttgtggc gaggctggtc gatgtctccc ccgatgggta cgcgcaaaac ctgacggatg
     2401 gcatcatccg cgcccgctat cgcaatttcg cgcgcggcga agcgccctcg ctgatcgagc
     2461 cgggaacagc ctatgaatac gatatcgacc tgtgggccac cagcaacgtc ttcaaagccg
     2521 ggcatcgcat tcgcgtggat attaccagca gcaatttccc gcgctgggac cgcaacccga
     2581 attcaggcca tgccttcggc gctgatagcg agacggtcgt agctcaccag accattctgc
     2641 acga
//
LOCUS       DASZAK010000043         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_107532, whole genome shotgun sequence.
ACCESSION   DASZAK010000043 DASZAK010000000
VERSION     DASZAK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107532"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1260
                     /locus_tag="VGT44_00725"
     CDS             <1..1260
                     /locus_tag="VGT44_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HEV2579346.1"
                     /translation="VFLNLGLLIVDEEQRFGVAHKELLKQMRSEIDVLTMTATPIPRT
                     LHMSLVNVRDLSVIETPPQERLPIRTTIREYDESLIREAILREIDRGGQVFFVHNRVQ
                     GIQVIAQRLQRLVPEARIAVGHGQLPEDQLERVMMDFSSGEYNVLIATTIIENGLDIS
                     NANTLIVNNSAMFGLSQLYQLRGRVGRSAHQAYAYFLYNKDSKLTPVQEKRLRAIFEA
                     TELGAGFRIAMKDLEIRGAGNLLGAEQSGFMNSVGFDLYTKLLAEAIEEQQGKRAETG
                     TPSVMLDLPLDAYLPDEYVGDRALKVKFYQRLANLSSPEQVEAMEAELSDRFGTPPQP
                     VKNLLAMVRLKVLAAKSGYEAISARDNEFVLTVRRTIVPNRIVLYRRFKNDARVQQGV
                     IHIPRRLLEPNWMEQLRDLLPKITSAA"
     gene            complement(1268..>1669)
                     /locus_tag="VGT44_00730"
     CDS             complement(1268..>1669)
                     /locus_tag="VGT44_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579347.1"
                     /translation="ALSRLASWRNVQMSLELDEQGNRTTQPDLAVDTAGQIAASLPAR
                     RSAEFAPAGVTTAPRANDVRVPGLERLIPQKRHQNRDILSLPLTRPQRLVYFLVDGKR
                     TLADISRCTGKNIQELDQILSELQAQGLILI"
BASE COUNT          364 a          473 c          493 g          339 t
ORIGIN      
        1 gtcttcctca acctgggatt gttgatcgtt gacgaggagc agcgctttgg agtcgcgcac
       61 aaggaactct tgaaacagat gcgcagcgaa atcgacgtgt tgactatgac tgcgactcct
      121 atcccgcgca cgctgcacat gtcgctggtg aacgtgcgcg acctgagcgt gatcgagacg
      181 ccgccccagg aacgcctgcc gatccgcacg accatccgcg agtacgatga aagcctcatt
      241 cgcgaagcca tcctgcgcga aatcgaccgg ggcgggcagg tcttctttgt gcataaccgc
      301 gtacagggca tccaggtcat cgcgcagaga ctccagcgcc tggtgccaga agcgcgtatc
      361 gctgtcgggc atggacagct gcccgaagac cagctcgagc gcgtcatgat ggacttctcc
      421 agcggggaat ataatgtgct gatcgcgacg acgatcatcg aaaatgggct ggatatctcc
      481 aacgccaaca cactgatcgt caataactcg gccatgttcg gcctctcgca gctgtaccaa
      541 ttgcgcgggc gcgtcgggcg cagcgcgcac caggcatacg cctacttcct ctacaacaag
      601 gatagcaagc tgacacctgt acaagagaaa cgcctgcgcg ccatcttcga ggcaaccgag
      661 cttggcgcgg gtttccgtat cgccatgaaa gacctggaaa ttcgtggggc gggcaatctg
      721 ctcggcgcgg agcagtcggg gttcatgaac tcggtcggct tcgatctgta cacaaagctg
      781 cttgccgagg ctatcgagga gcaacagggc aaacgcgcag agaccggtac gccctcagtg
      841 atgctcgatc tgccgcttga tgcctacctg cccgacgaat atgtgggaga tcgcgctctg
      901 aaggtcaagt tctatcaacg gctggccaac ctgtcgagtc ctgaacaggt tgaggcgatg
      961 gaagccgagc tgagcgatcg tttcggcaca ccaccccagc cggtgaagaa cctgctcgcg
     1021 atggtgcgtc tcaaagtcct ggcggcgaag tctggttacg aggccatctc agccagagat
     1081 aacgagtttg tcttgacggt gcgccgcacc atcgttccca accgtatcgt actgtaccgg
     1141 cgtttcaaga acgatgcgcg agtccagcag ggtgttattc acatcccacg ccgcctattg
     1201 gagccgaatt ggatggaaca attgcgcgat ctcttgccga agatcacctc ggcagcgtag
     1261 gggcatttca aattaggatc agcccctgtg cttgtaactc actcaatatc tggtcgagtt
     1321 cctggatatt cttgccggtg cagcgtgaaa tatcggcgag ggtacgcttg ccatcgacca
     1381 gaaaataaac aaggcgttgg ggtcgtgtca ggggtaacga taagatatca cggttttggt
     1441 gacgtttctg cggaatcaac ctttcgagac cgggaacacg cacatcgttc gcgcgcggcg
     1501 ctgttgtaac tcctgcaggt gcaaactcag ccgaccgccg cgccggtaag gatgcggcga
     1561 tctggccagc ggtatcaacg gccaggtcgg gctgcgttgt gcggttgccc tgttcgtcga
     1621 gttctaagga catttgtaca ttacgccagg aagctaagcg gctcagggc
//
LOCUS       DASZAK010000044         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_533382, whole genome shotgun sequence.
ACCESSION   DASZAK010000044 DASZAK010000000
VERSION     DASZAK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533382"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..>2207
                     /locus_tag="VGT44_00735"
     CDS             237..>2207
                     /locus_tag="VGT44_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV2579348.1"
                     /translation="MQSIARDYWSVWKQFVLQGVTQEDVLPPALMQSWRRCAASGLDP
                     YGEVAQEAETIDGASGVSQHMLALVRPVMEDLHQFVEGSECVVAFADADVCIVDCFGD
                     KDIQEELEHLGLHRGASWREEFQGSNAFSLALQESFPIQLDGAMHYRAALHPLYTSAA
                     PVHDLMGSAVGALGVIGRHEQCHPHTLGMITAAAQAISNQLQMQVWLNNANDLLAELK
                     SILQTLPEGVLLLRNDGVISQMNTPAGAMLGLVPARVNGRRLSDVLPLPVMLSQAITR
                     QQPLTDEEIVFETARGRVTCLCSLRPISNYHTDTSLADIVGSPASVGVIGATGLSLAD
                     PVTTDGYVLILRSIERVQRLVNRMTGARARLTFDNVVGATPALKEAMRLALLAARSNS
                     TVLLRGETGTGKEIFAQSIHNSSARVDGPFVAVNCAAIPRELINTELFGYEGGSFTGA
                     DRQGRSGKFEQAHGGTLFLDEIGDMPLDLQTALLRAIETRSIVRVGGQRVIPVDVRII
                     AATHRDLREEARHGNFRSDLFYRLNVLTIHIPALRERAGDIPLLAHHFLQRQSRALGR
                     SLTITPEAMEAIRGYNWPGNIRELENLLERVAYLMPGSVITLADLPLELQQAGEHVSA
                     LPARQGMPASESSQQPLALPPSPLAALANQGKA"
BASE COUNT          394 a          705 c          677 g          431 t
ORIGIN      
        1 ctgtcctggt ggaggtcatc ggcgggccaa cgcggacagg cacaaggccc ccgccccacc
       61 gctctaccac cccctgtccc tacgaacatt gtccgcattc aataatcaat gtgcattatc
      121 gactcctaag gtttcgttca cctctggcat tgtatcacgt attggggcgg cgatagctgt
      181 gctatactac agtgaattga tgatgtcggt aacggagacg gcataggttt tattacatgc
      241 agagcatcgc gcgcgattat tggagcgtct ggaaacaatt cgtcttgcag ggcgtgacgc
      301 aggaagacgt gctgccgcct gcgctgatgc agtcgtggcg gcgttgcgct gcgagcggcc
      361 ttgaccctta cggcgaagtc gcgcaggagg cggagaccat cgatggcgct tctggtgttt
      421 cgcagcatat gctcgcactg gtgcggcccg tcatggaaga cctgcaccag tttgtcgagg
      481 ggtcggaatg cgtcgtggcc tttgccgatg ccgatgtgtg cattgtcgat tgcttcgggg
      541 acaaagacat acaggaggaa ctggagcatc ttggcctgca cagaggggca tcgtggcgcg
      601 aggagtttca ggggtcgaac gccttctcgc tggcgttgca ggaatcattt cccatccagc
      661 tcgatggcgc tatgcattat cgcgccgcgc tgcacccgct ctacacttct gccgcgccgg
      721 tgcatgatct gatgggcagc gccgttggag cgctgggcgt gattggccgc cacgagcaat
      781 gccacccgca cacgctgggc atgattacgg cggcggcgca ggccatcagc aaccagttgc
      841 agatgcaggt ctggttgaac aacgccaacg acctgcttgc tgagctgaaa tcgatcctgc
      901 aaacgctgcc ggagggtgta ctcttattgc gcaacgatgg cgttatttcg cagatgaata
      961 cgcccgccgg ggccatgctc ggccttgtgc ctgcgcgcgt caacggcagg cgtctgagcg
     1021 atgtcctgcc cctgccggtc atgctctcgc aggccattac gcgccagcag ccgttgaccg
     1081 acgaggagat tgtctttgaa accgcgcgag gccgtgtcac ctgtctctgc tcgctgcggc
     1141 ccatctccaa ctaccacacc gataccagcc tggcggatat cgttggttcg cctgcctcgg
     1201 taggcgtaat tggtgcaacc ggtctgtcgc tggccgaccc ggtcacgacc gacggctatg
     1261 tcctgatcct gcgcagcatc gaacgagtgc agcggctggt gaaccgcatg accggcgcgc
     1321 gggcgcggct gaccttcgac aacgtcgtcg gcgcgacgcc cgcgttgaag gaggccatgc
     1381 gtctcgccct gctggcggcg cgcagcaact cgaccgttct gctacgcggc gagaccggca
     1441 cgggcaagga gatcttcgcg cagagcattc ataacagcag cgcgcgcgta gatggcccgt
     1501 tcgtcgccgt caactgcgcc gccatcccgc gcgaattgat caataccgaa ctcttcggct
     1561 acgagggtgg ctccttcacc ggcgcggacc ggcaggggcg ttccggcaag ttcgagcagg
     1621 cgcatggcgg cacgctcttc ctcgacgaaa tcggcgatat gccgctcgac ctgcagaccg
     1681 ccttgctgcg cgccatcgaa acgcgcagca tcgtgcgcgt cggcggccag cgcgtcatcc
     1741 cggtcgatgt gcgcatcatc gccgcgaccc atcgcgacct gcgcgaagag gcacggcacg
     1801 gcaacttccg ttccgacctg ttctatcgcc tcaacgtgct gaccatccac atccccgcct
     1861 tgcgcgagcg cgccggggat attcccctgc tcgcgcatca cttcttgcag cgccagagcc
     1921 gcgcgctggg gcgctctttg accatcacgc cggaagcaat ggaggccatt cgcggctaca
     1981 actggcccgg taatatccgc gaactggaga acctgctgga acgagtggcg tacctgatgc
     2041 ccggctcggt tatcacgctc gcggacctgc cgctcgaact acaacaggcc ggtgagcatg
     2101 tatctgctct accggcccgt caaggtatgc ctgcgtccga gtcaagtcaa cagccgcttg
     2161 cgctgccccc ttcgccgtta gccgcgttgg ccaaccaggg caaagca
//
LOCUS       DASZAK010000045         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_675396, whole genome shotgun sequence.
ACCESSION   DASZAK010000045 DASZAK010000000
VERSION     DASZAK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675396"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2502)
                     /locus_tag="VGT44_00740"
     CDS             complement(<1..2502)
                     /locus_tag="VGT44_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579349.1"
                     /translation="MQQATQIRCYYCGQAVNPWIDEDCPYCHYPASLEKEMSFLETSL
                     QYLRRSAAFGGARLTVGDVIQRYASRLDTLRRYKASPMPAEMIIALPQPAASAVEGHA
                     EKITAPVSSFMSAQPAAPRQQRPVFSLRSFLADQTINIVASLGAFLILLGALSFIATT
                     TNLLLSFVIVFAIHALFGGVGLSTYRFRSFRVVSLIYTMIFALLVPLVGFSAYRLIEG
                     HLIEIAPPTLIAVASVYAAIVYGLLAIYQRFKPFAYLGVVALAVADLALARTLGLGYW
                     WWPVMLMLLAFPMLLSIRQPSNNPDSTRGGLFTGRFAILQEPVRLLMFVFVGASVVGI
                     LLTTVAALAGLSVPDTQVRFALFTMSLLALAWTSLLLWMAQRRKEVIGVAFLFLASVL
                     AFCYAFDFQAIGYALALTLVALLYHGLSRFAARLLQALAMPGFHLDTIALWLVAVVPV
                     IGSPLLLNQLFARAFGDAGISQLFIDSSLLFHMGWESIAQLIAVILGALLVVDVAFCQ
                     GSIDRRRSAIHCAQVEASDQAHHQAHHQERGRNELRPYKPWLLLLAGFLLNVAYSLVV
                     LMLQVGPVWSFLALTIALVALAIVVRKRFGATWANPLDIIALAEAGLTLVLSLGLSLD
                     SIWMLLLFFAACSYGVVLYQRRHRLLFLPLVFALLATPILFYAQRTSVLLVAGVTLPF
                     VCAAIHRLISAKAQPAQVSVSPRLQLPVAWEWPLLCFGLLCGALVCIQDVSLSSSILQ
                     GWLGVHIPIAIEIGALALAWYAAAALASKHLWLIPVIAFAVVALVLPANPFWVLVGIT
                     PVAALLAVGVSRMAGRTWALPLYSVALLGGIMAGYA"
     gene            2665..3624
                     /locus_tag="VGT44_00745"
     CDS             2665..3624
                     /locus_tag="VGT44_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEV2579350.1"
                     /translation="MTVQPSHQRVSLADRGLRAIASTLVGSKSPPLPQRPAMGFFTDT
                     SLCIGCKACEIACKQWNQLPASDPNWSGSSYDNTEQLSATTWRHVQFIEQIGRKPDTA
                     NITDIRERIAQAKSNGAVPLSPTGTALPFFNSERWLMHSDVCKHCAHAPCLEACPTGA
                     IIRTEFDTVYVQQDICNGCGYCVVACPFGVIARDDHGSHTASKCTLCYDRLKDDMTPA
                     CAKACPTASIQFGEVGQLKARARERLGLLRERGVVEAQLYGANDAILEGGLNSFFLLL
                     DEPEVYNLPSNPSRPSTHVVPASLWTIVAAAILGLFGIVFFRK"
BASE COUNT          896 a         1121 c         1081 g          570 t
ORIGIN      
        1 cgcgtaaccg gccatgatcc cgccgagcaa ggcaacgcta tagagcggca gcgcccatgt
       61 tctccccgcc atacgactca cgccgacagc cagcagggcg gcaacgggag tgatacccac
      121 cagcacccag aatggattgg caggcagcac aagcgcgaca acggcgaacg cgatcacggg
      181 aatgagccat aagtgtttag acgcaagcgc ggctgccgca taccaggcaa gtgctagagc
      241 gcctatttcg atggctatag gaatatgcac tcccagccag ccctggagaa tactcgatga
      301 gagtgataca tcttgaatgc agacaagcgc gccgcacagc aggccaaagc acaacagagg
      361 ccattcccag gcaaccggca attgcagccg gggcgaaacg cttacctgcg cgggctgcgc
      421 cttcgcggag atcagccggt gaatggcggc gcacacgaaa ggcagtgtca cgcccgcgac
      481 gagcaggacg gaggtgcgct gtgcatagaa aaggataggc gtggccagca gcgcaaacac
      541 caggggcagg aacaacaaac gatggcggcg ctggtacaac acaacgccat aagaacaagc
      601 agcgaagaag agcagcagca tccagatgct atccaggcta agccccaggc tcagcacgag
      661 cgtcaagccc gcctctgcga gcgcgatgat gtccagggga ttcgcccatg tcgcgccaaa
      721 acgcttgcgc actacgatag cgagagcgac cagcgcaatc gtcagcgcca ggaaactcca
      781 caccggcccg acttgtaaca tcagcacaac caggctatag gccacgttca gaagaaaacc
      841 ggccagtaat agcagccagg gcttgtaggg gcgcaattca ttgcgccctc tttcctggtg
      901 atgcgcctgg tgatgcgcct ggtcactcgc ctccacctgg gcgcaatgaa ttgcgctcct
      961 acgccggtcg attgaacctt gacaaaacgc tacatcaacg accaggagcg cgccgagtat
     1021 gacggcgatg agttgcgcaa tcgactccca gcccatatga aagaggaggc tggagtcaat
     1081 aaagagctgg gatatccctg catcgccgaa tgccctggca aagagctgat tcagcaagag
     1141 gggcgaacca atcacaggca cgacagcgac cagccatagc gcgatggtgt ccaggtggaa
     1201 accaggcata gcgagcgctt gtaggagtcg cgcagcaaaa cggctgaggc catgatagag
     1261 cagcgccacc agcgtcagcg ccagggcata gccgatcgcc tggaagtcga aggcatagca
     1321 gaaggcaaga acgctcgcca ggaacaggaa cgccacaccg atcacttcct tgcgacgctg
     1381 cgccatccac agcaggagcg atgtccaggc cagcgcgagc agcgacatgg tgaacagcgc
     1441 aaaacgcacc tgtgtgtccg gcacgctcag acctgcaaga gcggctacag ttgtgagtag
     1501 gatgccgacc acgctcgcgc ccacaaagac aaacatcagc aggcgcacgg gttcttgcaa
     1561 gatggcgaat cgtccggtga acaggccacc tctcgttgaa tcggggttgt tcgacggctg
     1621 gcgaatggag agcagcatcg ggaaggccag tagcatcaac ataaccggcc accaccagta
     1681 gcccaggccc agcgtccgag ccaacgccag gtctgcgacc gccagggcaa ccacgcccag
     1741 gtaggcgaac ggcttgaagc gctggtatat cgccagcagg ccatagacga tggcggcgta
     1801 gaccgaggca accgcgatca gggttggcgg ggcaatttcg atcagatgcc cctcgatcag
     1861 tcgataggct gagaacccga cgagcgggac cagcagggca aaaatcatgg tataaatcag
     1921 cgagacgacg cggaaggagc ggaaacgata ggtgctgagg ccaacccctc caaagagcgc
     1981 gtggatggca aacacaatca cgaaagacag cagtagattg gtcgtcgtag cgataaaact
     2041 gagcgcgccc aacaaaatca gaaacgctcc cagcgaggcc acaatattga tggtctgatc
     2101 tgccaggaac gagcgcagcg agaagacagg acgctgctga cgaggggctg cgggctgcgc
     2161 cgacatgaaa gatgaaactg gcgcggtgat cttctccgcg tggccctcaa ccgccgaagc
     2221 agccggttgg ggaagcgcga taatcatctc cgcgggcatc ggactggcct tgtacctgcg
     2281 cagcgtgtcc aggcgtgacg catagcgctg gatcacatcg ccaacggtca gcctcgctcc
     2341 cccaaaggca gcgctgcgcc gcaggtattg cagcgatgtt tcaagaaacg acatctcctt
     2401 ctctaaagag gccggataat ggcagtaggg acagtcctca tcaatccagg gattgaccgc
     2461 ctgaccgcag tagtagcagc ggatttgagt tgcctgttgc atatgatgac tcctttgaac
     2521 cctcgctcct ggcatggcgc acgataatgt ggtataaaca ctgtataaaa gcagtccagc
     2581 agggttctga acaagtataa ttgctggaca aagccgaggg tgagtgtcat atgtcctatt
     2641 tcattccata gaaggagtga ctgtatgact gtccaacctt ctcaccagcg cgtttccctt
     2701 gccgaccggg gattgcgcgc catcgcctcc acgctcgtcg gcagcaaatc gccaccactg
     2761 ccgcagcgac ctgctatggg ctttttcacc gataccagcc tgtgcatcgg ctgcaaagcc
     2821 tgcgagatcg cctgcaagca gtggaaccag cttccggcca gcgatcccaa ctggagcggg
     2881 tcgagctacg ataataccga gcaactgagc gccacgacct ggcggcacgt gcaattcatc
     2941 gagcagatcg ggcgcaagcc agataccgcg aatattacgg acattcgcga gcgcatcgct
     3001 caggcgaaaa gcaacggcgc tgtgccgctc tcgccaacgg gaaccgcgct gcccttcttc
     3061 aatagcgagc gctggctgat gcacagcgac gtgtgcaagc actgcgccca cgcgccctgc
     3121 ctggaagcct gcccaaccgg cgcgatcatc cgcacggaat tcgataccgt ctatgtccag
     3181 caggatatct gcaacggctg cggctactgc gtcgtggcct gccccttcgg ggtcatcgcg
     3241 cgcgacgatc acggcagcca caccgccagc aaatgcacac tgtgctacga ccgcctcaaa
     3301 gacgacatga cgccggcctg cgccaaagcc tgcccgaccg cctcgatcca gttcggcgaa
     3361 gtggggcaac tcaaggcgcg cgcccgcgaa cgcctgggcc tactgcgcga gcgcggcgtc
     3421 gtcgaggcgc agctctacgg cgcaaatgac gccatcctcg aaggcggcct gaactcgttc
     3481 ttcctcctgc tcgacgagcc ggaggtctac aacctgccat ccaacccatc gcgcccctcg
     3541 acccacgtcg tccccgcctc gctctggacg attgtcgcgg cggcaatcct gggcctgttc
     3601 ggcatagtat ttttcaggaa ataattgtcg cacaccatgc gtagggacag ggggtagtcg
     3661 ggtggagc
//
LOCUS       DASZAK010000046         4032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_284996, whole genome shotgun sequence.
ACCESSION   DASZAK010000046 DASZAK010000000
VERSION     DASZAK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284996"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VGT44_00750"
     CDS             <1..544
                     /locus_tag="VGT44_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEV2579351.1"
                     /translation="RAAAYGPLSVMVPMIATLQDWQQARDIAEGVRQELNAPHTPLGI
                     MIEVPSAAILADLFAQEVDFFSIGTNDLTQYTLAMDRLHPQLAKQADGLHPAVLRMID
                     NTVQAARRAGKWVGVCGGIAGDPKGAVILAGLGVSELSVGTPSVAAIKAHIRGQSMAA
                     MQQLAKQALQCRTAEEVRSL"
     gene            541..1470
                     /gene="pfkB"
                     /locus_tag="VGT44_00755"
     CDS             541..1470
                     /gene="pfkB"
                     /locus_tag="VGT44_00755"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV2579352.1"
                     /translation="MKIATVTLNPAIDQTVRVDHFLSNTVNYAQTMQFDAGGKGVNVA
                     SFLADAGFTVSVTGFLGRDNSEVFERYFAVKGIDDRFVRIPGNTRIGVKIVDEANQQT
                     TDINMPGLSPFEGAMEKLFERVEQLTASCDWFALSGTLPPGVPQNTYAALITLLKKHG
                     KRVVLDTSRDALREGLQAVPTIVKPNIDELHQLTGLSLLDEVALEQAARTLLERGIEL
                     VVISMGERGAMFVNATSSLVAVPPPVMVKSTVGAGDAMVAGIIAGQARELSLPDCARL
                     ATAFSLGAITQVGHKLPDQAAIQAYFQRVSLRY"
     gene            1549..2991
                     /locus_tag="VGT44_00760"
     CDS             1549..2991
                     /locus_tag="VGT44_00760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HEV2579353.1"
                     /translation="MANIVAITSCPTGIAHTFMAAEGLQRGAEALGHTIKVETQGSVG
                     AQNTLTEADVQKADVVIIAADTKVEMSRFAGKPVYETSTNAAIKDGQAVVKSALAQAA
                     ASPVASGRPDYVGQVQAAKAAQTRSRRGPYKHLLTGVSYMIPFVVSGGILIALSFAIA
                     GYQVVFQPTTSQSFGYALFVIGAKAGFALFVPILSGYIAYSIADRPGLAPGMIGGILA
                     GLTGSGFLGGIIAGFLAGYTVYWLNRTIKLPRNFAGLMPVLILPLLGSLIVGLVMFYV
                     VGWPVSQINLGLTNWLKSIQSTYAVLLGLVLGLMMAFDMGGPVNKAAYAFSVGLLGSG
                     VTMPMAAVMAAGMTPPLGLALATVLFKSRFTDDEREAGKAAWVLGLSFITEGAIPFAA
                     EDPFRVIPSIMIGSAVTGALSMLFQVQLHVPHGGVWVIFIPNVVTNLFPYILSILVGT
                     AVTSGMLFILKRPIAATEQSEEGAGVAAAA"
     gene            3320..>4032
                     /locus_tag="VGT44_00765"
     CDS             3320..>4032
                     /locus_tag="VGT44_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2579354.1"
                     /translation="MKYCALCHAELVENSLFCGNCGTAINVLADGFTRMSMPGKAPVT
                     PAFPVSPAPPLSSNPGQYSQQYAPGESNIPTLPISEEEEERRRRAAMMGLGLLSAVQQ
                     GNGAPMVQGTPQIGGVPTVQGTPQVQSGSFQNAQPGPQSGAIQVAPHTPPIHTPVSPT
                     PPIHSSPTLTLHHHPQGGSPSGSSGSAPKPGCGPGLIITAIIIPIVIIASIIGLGLTI
                     FAPDITLSGSTSVSMGASLT"
BASE COUNT          811 a         1217 c         1182 g          822 t
ORIGIN      
        1 ccgcgccgct gcctatggcc cgctcagcgt catggtcccc atgattgcga cgctgcaaga
       61 ctggcagcag gcgcgcgata tagccgaagg ggtgcgtcag gagttgaacg ctccgcacac
      121 cccgctcggc atcatgatcg aggtgccttc ggcggcgatc ctggctgacc tgttcgccca
      181 ggaagtcgat ttcttctcaa tcggcacgaa cgacctgacg cagtataccc tggccatgga
      241 tcgcctgcac ccacaactgg cgaaacaggc cgatggactg cacccggcgg tgctgcgcat
      301 gatcgacaac accgtccagg ccgccaggcg agcgggcaag tgggtgggag tctgcggcgg
      361 catcgcgggc gacccgaaag gcgcggtgat cctggccggg ttgggcgtct cggaattgag
      421 cgtcggcaca cccagcgttg ctgccatcaa agcgcacatt cgcggccaat cgatggcagc
      481 tatgcagcag ctcgcgaagc aagcattaca atgccgcacg gcggaggagg tccgcagctt
      541 atgaagatcg ctaccgtaac gctcaacccc gctatcgacc agacggtgcg ggtcgatcat
      601 ttcctatcca acacggtcaa ttacgcgcag accatgcaat tcgacgcggg cggcaaaggc
      661 gtcaacgtcg catcgttcct ggcggatgcc ggttttacag tgagcgtgac aggcttcctc
      721 ggtagggaca actccgaggt cttcgagcgc tacttcgcag tcaaaggcat tgatgaccgc
      781 ttcgtgcgta tccctggcaa cacgcgcatc ggcgtcaaaa tcgtggacga ggcgaaccag
      841 cagacgaccg acatcaatat gccgggccta tctcccttcg agggagcgat ggagaagctg
      901 ttcgagaggg tcgagcagct cacggcaagc tgcgactggt tcgcattgtc aggtacgctt
      961 cctcccggcg tcccgcagaa tacctatgct gcgctcatca ccctgctcaa aaagcatggc
     1021 aagcgagtcg tgctggatac cagtcgcgac gcgctgaggg aaggtttgca ggccgtgcca
     1081 accatcgtca agcccaacat cgatgaactg catcaattga ccgggctgtc gctgctggat
     1141 gaagtggcgc ttgaacaggc ggcgcgcacc ctgcttgaac gcggcatcga actggtggtc
     1201 atctcgatgg gcgagcgcgg cgcgatgttc gtgaacgcga cctcctcgct ggtcgcggtt
     1261 ccaccgcctg tcatggtgaa gagcacagtc ggcgcaggcg atgcaatggt ggcgggcatc
     1321 atcgcaggcc aggcgcggga gttgagcttg cccgactgcg cgcggctggc aaccgccttt
     1381 tcacttggag cgatcaccca ggttggtcac aagctgcccg atcaagcagc gatccaggcg
     1441 tattttcagc gcgtttcgct ccggtactga tctatcgttc ctcgtagtgc ggatgaatta
     1501 tctatcgtaa cgacactggt tacttcttca aagaaaggat gcttagtcat ggcgaacatt
     1561 gtcgcaatca cttcttgtcc aacgggcata gcccacacgt tcatggcggc agaagggcta
     1621 cagcggggcg cggaagcgct ggggcacacg atcaaagtgg aaacccaggg ttccgtcggc
     1681 gcgcaaaaca cattgaccga ggccgatgta caaaaagcgg acgtcgttat tatcgcagcc
     1741 gatacaaaag ttgaaatgag ccgtttcgct ggaaagccgg tctacgaaac gtccaccaac
     1801 gccgccatca aggatggcca ggcggtcgtc aaaagcgcgc ttgcccaggc agccgccagc
     1861 cctgttgcat caggcaggcc ggactacgta gggcaggttc aggccgccaa agccgcgcag
     1921 accaggtcgc gccggggtcc gtacaagcac ctgctgacag gcgtctcgta catgatcccc
     1981 ttcgtcgtat cgggcggtat cctgatcgcc ctgtcgtttg ccatcgcggg ctaccaggtc
     2041 gtcttccagc caacgacttc gcagtccttt ggatacgcgc tctttgtcat cggcgcaaag
     2101 gccggcttcg cgctcttcgt ccccattctc tccggctata tcgcctactc gatcgccgac
     2161 cggccaggcc tggcgcccgg catgatcggc ggcatcctgg cgggtctgac cggctctggc
     2221 ttcctgggcg gcatcatcgc gggcttcctg gcgggctata ccgtctactg gctgaatagg
     2281 actatcaaat tgccgcgcaa cttcgccgga ctaatgcccg tgctgatcct gccgttgttg
     2341 ggatcgctga tagtcggcct ggtgatgttc tacgtcgtcg gctggccggt cagccagatc
     2401 aacctgggct taaccaactg gctcaagagc attcaaagca cttacgccgt tcttctggga
     2461 ttggtgctgg gcttgatgat ggccttcgac atgggcggcc cggtcaacaa agcggcctat
     2521 gccttctcgg tcggactgct tggcagcggc gtaactatgc cgatggcggc ggtgatggcg
     2581 gctggcatga ctcctccgct cggcctggcg ctggcgacgg tgctgttcaa gagccgtttc
     2641 acggatgacg agcgtgaggc cggcaaggca gcctgggtgc ttggcctgtc cttcatcacc
     2701 gagggcgcga ttcccttcgc ggcagaagac cccttccgcg tcattccgtc gatcatgatc
     2761 ggttcggcag tgaccggggc gctgtcgatg ctcttccagg tccagttgca cgtcccgcac
     2821 ggcggcgtgt gggtcatctt catcccgaac gtcgtgacca acctgttccc ctacatcctg
     2881 tcaatcctgg tcggtacagc agtgacatcg ggtatgcttt tcatcctcaa gcgccccatc
     2941 gctgccaccg agcaatcgga ggaaggagca ggcgtcgcag ctgccgcata aaggtaggta
     3001 gaacgcaaag cgatacatga gcgctgggcg aaatctacaa cactgtaagg gatagcatag
     3061 attgaagggc gcaaccgcga ggatgcgccc ttcaaactaa tatgatctga tccactttgc
     3121 agatgtgagc taagcggctt tcttcattca cgcattctgc tagactggta aggagacaaa
     3181 caggctacaa gcggctcgaa cgcgataatt cggtctcatt tcttcgtata caagagtata
     3241 tacatctttt ggcctgtccc aattgtctct ctcgttattg gctctatgat atacccttcc
     3301 acgtggagga tgctattgta tgaagtattg cgcattgtgt catgccgagc tggtcgagaa
     3361 ctcgctattt tgcgggaact gcggcacggc gatcaacgtt ctggctgatg ggtttacgcg
     3421 catgagcatg cctggaaagg cgcctgtaac tcccgcattt cctgtttctc ctgcgccacc
     3481 cctttcctct aatcccggtc aatactccca gcagtatgcg ccgggtgaga gtaacattcc
     3541 caccctgccg atcagcgagg aagaagagga gcggaggaga cgcgccgcca tgatgggcct
     3601 ggggctgctc agtgcggttc agcagggaaa tggagcgccg atggtacagg gaacgccgca
     3661 gataggcggc gtcccaacgg tgcagggaac gccccaggtt caaagcggct ctttccagaa
     3721 cgctcagcct ggccctcaaa gtggcgcgat tcaggtagcg ccacacacac cacctatcca
     3781 tacgccagtt tcaccaactc ccccgattca ctcgtcgccg acgctgacgc tgcatcatca
     3841 cccgcaaggc ggatcaccat ccggttcgag cggctcagct cccaaacctg gctgcggacc
     3901 cggcctgatt atcaccgcca tcatcatccc catcgttatt atcgccagca ttatcggttt
     3961 gggattgacc atcttcgcgc cagatataac gctcagtgga agcaccagcg tcagcatggg
     4021 tgcttcgctg ac
//
LOCUS       DASZAK010000047         1569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_36298, whole genome shotgun sequence.
ACCESSION   DASZAK010000047 DASZAK010000000
VERSION     DASZAK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36298"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="VGT44_00770"
     CDS             <1..626
                     /locus_tag="VGT44_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579355.1"
                     /translation="GRARVSILPKGDSNDDAVLRRFVAAGIKIAPGATGTAVYIQDYA
                     RAVVNAFIQAGVLSFVAICGLLLIVLRKIRDVAVTMAPIILTGLLTMGICVVIRQPLN
                     FANIIALPLLFGIGVAFHIYFVMSWRAGGSHLLTSSLARGVFFSALATATGFGSLWAS
                     KDPGTASMGKLLMISLVWTLVSALIFQPALMALRVCTRAASAARTTS"
     gene            complement(616..1221)
                     /locus_tag="VGT44_00775"
     CDS             complement(616..1221)
                     /locus_tag="VGT44_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2579356.1"
                     /translation="MVTRFAMFLCVLVLGPVGNSAYATASEPAVAQVQTLSSALLAAM
                     KAGPRASMMERYRQLKPVVEQTFALPFMTRLSVGPDWAKFSPQQQKALVAAFSRYTIA
                     NYAHNFHDFNGQRFDVDANVITRGPDKVVRTSITSTDGTATNLLYRMRDVDGAWKIVD
                     VFFNGVSQLTLHRVDFTSAIGSGGAPALIAYLNKLSDGLMK"
     gene            complement(1276..>1569)
                     /locus_tag="VGT44_00780"
     CDS             complement(1276..>1569)
                     /locus_tag="VGT44_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV2579357.1"
                     /translation="WRAGGSHLLTSSLARGIFFSALATATGFGSLWASKHPGTASMGE
                     LLMISLIWTLASALVFQPALMSLANPKTPVRVIAAGPIFALGRSPAHQERRDP"
BASE COUNT          286 a          477 c          508 g          298 t
ORIGIN      
        1 acggacgggc gcgcgtttcg atactgccca agggagattc gaacgacgat gcggtactgc
       61 gccgattcgt cgctgctgga atcaagatcg cgccgggcgc gaccggcacg gcggtctaca
      121 ttcaggacta cgcccgggcc gtggtcaatg cattcatcca ggcgggggtg ctgtcattcg
      181 tcgccatctg cggcctgctg ctgatcgtgc ttcgtaagat acgtgacgtg gcagtcacca
      241 tggcaccgat catactgacg gggctcctga ccatgggcat ctgcgtagtc atccggcagc
      301 cgctcaactt cgccaatatc atcgccctgc cgttgctgtt cggaatcggc gtggcgttcc
      361 atatctactt cgtcatgtcc tggcgcgcgg gcggctcgca cctcctgacg tcgagcctgg
      421 ctcggggggt attcttcagc gccctcgcga ccgccacggg cttcggcagc ctctgggcct
      481 cgaaggatcc cggaaccgcc agcatgggca agctgctgat gatctcgctc gtgtggacgc
      541 tcgtgtcggc gctgatcttc cagccggcgc taatggccct gcgcgtctgc accagggcgg
      601 cgagcgccgc ccggactact tcatgagacc gtcgctgagt ttgttcaggt aggctatcaa
      661 ggcgggagcg cctccggagc cgatcgccga agtaaaatcc accctgtgca gggtcagttg
      721 gctgaccccg ttgaaaaaga cgtcgacgat cttccaagcc ccgtcgacgt cgcgcatgcg
      781 ataaagcagg ttcgtggccg tcccatctgt agacgtgatg ctggtccgga caaccttgtc
      841 cggaccgcga gtgatcacgt tggcatcgac gtcaaagcgc tgtccattga agtcgtgaaa
      901 attgtgagcg taattggcga tggtgtagcg gctgaaggct gcgaccaagg ccttctgctg
      961 ctggggcgag aacttggccc aatccgggcc cacggacagg cgggtcatga acggcaacgc
     1021 gaaagtctgc tcgaccacag gcttgagctg gcgatatctc tccatcatgg acgcgcgcgg
     1081 acccgctttc atggccgcga gcagcgcgct cgacaaggtc tgaacctgcg cgacagcggg
     1141 ttcgcttgcc gtggcatagg cagaattccc gacggggccc agaacgagca cacagagaaa
     1201 catcgcgaat cttgtgacca tggtttggct ctcgcgcgtg ccggggcgcc tgacgatagc
     1261 acaatagccg atcaatcagg ggtcgcgcct ttcctgatga gcagggctgc ggcccaacgc
     1321 gaagatggga cctgcggcga tcaccctgac cggtgtcttg gggttggcga ggctcatcaa
     1381 ggccggctgg aagaccaacg ccgaggcgag tgtccatatg agggatatca tcagcagctc
     1441 acccatgctg gccgttccgg gatgtttcga ggcccagagg ctgccgaagc cggtcgccgt
     1501 cgcgagcgcg ctgaagaata ttccgcgggc gaggctcgat gtgagtaggt gcgagccgcc
     1561 cgcgcgcca
//
LOCUS       DASZAK010000048         5268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_142925, whole genome shotgun sequence.
ACCESSION   DASZAK010000048 DASZAK010000000
VERSION     DASZAK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142925"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2092)
                     /locus_tag="VGT44_00785"
     CDS             complement(<1..2092)
                     /locus_tag="VGT44_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEV2579358.1"
                     /translation="MQTVLIANRGEVAVRVARATAELRIRTVMVYSEDDERSLHVRKG
                     DVALPLRGRGATAYLDIKQLLDAARLSGCDAVHPGYGFLSENAHFASRCAEEGLTFVG
                     PQPEVLQLFGDKARARLLAKQEGVAVIPGTTHATSLEEARAFLESLGVGGAVMIKALA
                     GGGGRGMRAVYRPEDLAEAYARCASEARAAFGADGLYVEQLIRHARHIEVQIVGDRLG
                     EVIDLGERECTLQRRHQKLVEVAPSPSLTPHQRRHIITAALQLARAAQYHNLGTFEFL
                     LDAGREQDDAPLYFMEANPRLQVEHTVTEQVRGVDLVKTQLLLAAGSSLAEIGMQAGG
                     TVTGCSVQARINMETLDSAANAHPSAGTLTAFEPPTGPGVRVDTFAYSSYTPSQRFDS
                     LLAKVIASVPSGGYAEALARAYQALCEFRIEGIQTNIPFLQNLLSHPDVVANRVDTHF
                     VEEHMPELLAPSEAHRRLYFSHAHVEGESPSAAVSFDYPEGSVPLPAPHQGAVVSIDV
                     QAGDRVANGQQVAVLEAMKMEFIVEAITGGVVREIAVAPGTSVAAGQPLLFLEPAEVA
                     DMTQGQSSAPDLDTIRPDLREARARHEVGLDDARPAAVERRRRSEQRTARENIADLCD
                     PESFLEYGALALAGQRRRRSQEELIAISPADGLVAGVGSINGAIFPEARARCMALAYD
                     YTVFAGTQGVMNHKK"
     gene            2309..2863
                     /locus_tag="VGT44_00790"
     CDS             2309..2863
                     /locus_tag="VGT44_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579359.1"
                     /translation="MRKALSRTLYLIGLVICIVGGILLGVSFAGSTTGIDPNTGATTI
                     TSIGNPALFGIGIFLLVIGGILGFIAYVGALIKTAMLGRWGWFVCLLVISGITMLVYI
                     FAGPETRPAAQVPMGYPPQAYPPPPGYPPQGYPQPGYPPQQGYPPQPGYPQQGNPPPY
                     PPSGYPQSDNPPQGGYPPVDYPRP"
     gene            2885..4099
                     /locus_tag="VGT44_00795"
     CDS             2885..4099
                     /locus_tag="VGT44_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide family glycosyltransferase"
                     /protein_id="HEV2579360.1"
                     /translation="MAKVLFFNLPAYGHTNPTLPLVAELVHRGEDVIYYSSEAFRPAI
                     GQAGATFRGVDAFFNEQTYIDPNLARTAYTLIRATQEIIPAILADVTADKPDYIIFDS
                     LCVWGRCIAQILRVPAIASITTLARPRSLLHPEVLATLPAVIPMTIRMTFQARKELPK
                     FQAIARQLQETYHIPRPQFGDVFNNLAELNIVYSTRQLQIYPNSFNDTFKFVGPFLNN
                     RVEAPSFPFDELGNEPVVYISLGTVFNDNGDFYRLCVEAFADRDYRVVMSVGSKMDMT
                     RLGTIPRNFIVKAFVPQLQLLQRAALFITHCGMNSVSEGLSAGVPLLMIPQAADQFFL
                     APRLQRLGAGKMLRATQLNAPRLRKAAEEILAQRAFQQRSAIIGASFREAGGPTLAVD
                     EIEAFKRSKLIR"
     gene            4227..5078
                     /locus_tag="VGT44_00800"
     CDS             4227..5078
                     /locus_tag="VGT44_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2579361.1"
                     /translation="MSQANASPEIGQSIQAGGVMTNYHEAGSGSPVILIHGSGPGVSA
                     WANWQFAIPFLAQRLRVFAYDQLGFGYSEAAPNQSYSLKRWTDHLLNFMQAVGVRRAH
                     LVGNSMGAAVALAAAVTQPEAVDRLVLMGAMGVRFNLKRGEGLDAVWGYTPSVENMRR
                     LIDLFAYNPGRFPTEQLAKLRYEASIRPGAQEAFSSMFPEPRQKQVEALAAYEDHLSD
                     VQNPTLIIHGREDRVIPIETSLKLINTLPNAQLHVFGHCGHWTQLEPRDTFNRLVRDF
                     LTEETSG"
BASE COUNT         1067 a         1648 c         1450 g         1103 t
ORIGIN      
        1 atttcttatg gttcatcacg ccctgcgtcc cggcaaagac ggtgtagtcg taggccagcg
       61 ccatacagcg cgcccgcgcc tctggaaaga ttgcgccatt gatgctgcca actccagcca
      121 ccaggccatc cgccgggctg atcgcgatca gttcttcctg tgagcgccta cgtcgctgac
      181 cggccagcgc cagcgcgcca tactcgagga aactctcagg atcgcacagg tctgcgatat
      241 tctcgcgcgc tgtgcgctgc tcgctccgtc tcctgcgttc cacggctgcc ggacgtgcgt
      301 cgtccagccc gacctcgtgc cgtgcgcgcg cctcccgcag gtctgggcgg atggtatcca
      361 ggtcgggcgc gctgctctgt ccctgggtca tgtcggcgac ctcggcgggt tcaagaaaca
      421 gcaaaggctg tcctgcagct acgcttgtgc cgggtgcaac cgctatctct cgcaccacgc
      481 cgccagtaat cgcctccaca atgaactcca tcttcattgc ctccagcacc gcaacctgct
      541 gcccatttgc tacacgatct cctgcctgca cgtcgatgga gacgaccgcg ccctggtgcg
      601 gagcaggcag tggaacgctt ccctccgggt aatcaaaaga aacggcggcg gatggacttt
      661 ccccttcgac gtgggcgtgg ctgaaataca agcggcggtg cgcttcggag ggcgcgagca
      721 attcgggcat atgttcttca acaaagtggg tatcgacgcg gttggcgacg acatccgggt
      781 ggctcaacag gttttgcaag aagggaatat tggtctggat gccctcgatc ctgaattcgc
      841 acagagcctg gtacgcgcgc gccagagctt ctgcatagcc gccagaggga acgcttgcaa
      901 tcactttggc gagcaaagag tcgaaacgct gactgggcgt gtaactgctg taggcaaagg
      961 tgtcaacgcg tacaccgggg cccgtcggcg gctcgaaggc cgtcagcgtc cctgccgatg
     1021 ggtgcgcgtt ggcggcgcta tccagcgtct ccatgttgat gcgcgcctgc accgagcagc
     1081 ccgtcaccgt gccgcctgct tgcattccaa tttccgccag cgaacttccc gccgccagca
     1141 acagttgggt cttgaccagg tcaaccccgc gcacctgctc ggtgacggta tgctcgacct
     1201 gcaagcgtgg gttggcctcc ataaaataga gcggcgcgtc atcctgctcg cgcccggcat
     1261 ccagcagaaa ctcgaatgtc cccaggttgt gatactgcgc cgctcgcgcc agctgcaggg
     1321 ccgctgttat gatgtgccgg cgctgatgcg gcgtcaggct gggactggga gcgacctcga
     1381 ccagtttctg atggcggcgc tgcagtgtgc attcgcgctc gcccaggtcg attacttcac
     1441 ccaggcgatc accgactatc tgcacctcga tgtggcgggc gtgacgtatc aactgctcca
     1501 catataaccc atccgctcca aaggctgccc gcgcctcgga cgcgcagcga gcatacgcct
     1561 ccgccaggtc ttccgggcgg tagactgcgc gcatcccgcg tcccccgccg ccagccaggg
     1621 ctttgatcat cacagctccc ccgactccca ggctctccag gaacgcccgc gcttcttcca
     1681 ggctggtcgc gtgcgtagtc cctgggatta cggcaacgcc ttcctgcttc gccagcagac
     1741 gcgcgcgggc tttgtctccg aagagctgga ggacctcagg ctgcggtcca acgaaggtca
     1801 acccctcctc tgcgcagcgc gacgcaaaat gggcattctc actgagaaag ccatagccgg
     1861 gatgcactgc atcgcagccc gagagccgcg cggcgtccag tagttgcttg atgtcgagat
     1921 aagcggtggc tcccctacca cgtaagggca acgccacatc tcctttgcgt acatgcaggg
     1981 agcgttcgtc atcttctgaa tagaccatca ccgttcggat gcgcagttcg gcggtggcgc
     2041 gcgcaacgcg tacggccact tccccgcgat tggcaatcaa cactgtctgc atagacgcat
     2101 gactcttttc tttcgcgata tttcctgatc ggatgagtac gatcatatgc ctgtctcatc
     2161 ttttccgtca agtagatgca tagagttctg aaaggcttac tctgccggaa tttccacgat
     2221 gcttgacgtt catacccatg agtcatatac ttcagatgtc gaacgcccca ttgatcgtca
     2281 tcatcattcg ttctgttggg aggctcttat gcgtaaagcc cttagtcgca ctctctacct
     2341 tatcggtctg gtaatctgca tcgtgggtgg tattctgctt ggcgtaagtt ttgcgggaag
     2401 cactaccggc attgacccaa acaccggagc taccacgatc acatcgattg gaaatcccgc
     2461 cctgtttggc ataggcatct ttctattagt gattggcggc atactgggct tcatcgccta
     2521 tgtcggggcg ctcatcaaaa cggccatgct aggtcgctgg ggctggttcg tctgtcttct
     2581 ggtgatctcc ggcatcacca tgctggtata tatctttgcc ggaccggaga cacgccccgc
     2641 cgcgcaggtg ccaatgggct acccgccgca ggcgtatccg ccgccgcccg gttatccgcc
     2701 gcagggatat ccccagccag gttatccgcc ccagcagggc tatccgccgc aacccggcta
     2761 cccccagcag ggtaatcccc caccctatcc accatcgggg tatccccagt cggataaccc
     2821 accgcaggga ggctatccgc cggtagatta tcctcgtccg tagggtaagc aggtaaaaaa
     2881 agccatggcg aaagtgctgt tcttcaacct tcccgcctat ggtcatacga atccgacgct
     2941 gccactggtc gcggagctgg tccatcgtgg cgaggatgtc atatattatt cttctgaggc
     3001 attccggcct gccatcgggc aggccggagc cacctttcgc ggcgtcgatg ccttctttaa
     3061 cgagcaaacg tatattgacc ctaacctggc gcgcaccgcc tataccctga tacgggcaac
     3121 gcaggaaatc atccccgcca ttctcgcgga tgtgaccgcc gacaaaccag actacattat
     3181 ctttgactcg ctctgcgtct ggggcaggtg cattgctcag atattgcggg tgccagcgat
     3241 tgcctcgatc accacgcttg ccagaccacg ctcgctgctg catcccgaag tgcttgctac
     3301 ccttccagcc gtcataccca tgacgatacg catgacgttt caagcgcgca aagaactgcc
     3361 aaaatttcaa gcgatcgcca ggcaactgca agaaacgtac cacattccca ggccgcaatt
     3421 cggcgatgtc ttcaataatc tcgccgaact caacatcgtc tactcaacca ggcagcttca
     3481 aatctacccc aactcgttca atgacacatt caaattcgtt ggaccctttc ttaataaccg
     3541 tgtggaggct ccatcattcc catttgacga attggggaac gagccggtcg tttatatttc
     3601 gcttggaacc gtctttaacg acaacggaga tttctaccgc ctctgcgtcg aagccttcgc
     3661 cgaccgtgac tatcgcgtgg ttatgtcggt tggcagcaag atggacatga cgcgcctggg
     3721 aacgattccc cggaatttca tcgtcaaagc ctttgtgcct caactccaac tcttgcagcg
     3781 cgccgcgctc ttcatcaccc actgcggcat gaatagcgtg agcgagggcc tttccgcagg
     3841 cgtcccgctc ctcatgattc cacaggccgc cgatcagttc ttccttgccc cgcgtctcca
     3901 gcgcctcggc gccggtaaaa tgctgcgcgc cacacaactg aacgcgcccc gtttgcgcaa
     3961 agcagccgaa gaaatcctgg ctcagcgcgc gttccaacag agaagcgcaa ttatcggcgc
     4021 atcattccgt gaagctggcg ggccaacgct cgctgtagac gagatagaag cattcaagcg
     4081 atcgaagcta atcaggtagg ttggctggct atcaacagca tcgtgcaaac aaaagcaata
     4141 tatgttatgt tatgcagtaa ccttgttcaa aacagcaaac aacaaccgat agtcaccgta
     4201 gtagcgccag aaaggataag aactcgatgt cacaggcaaa tgcgtcgccc gaaatcggcc
     4261 agtcgattca ggccgggggc gtgatgacga attatcacga ggcgggcagc gggagtcccg
     4321 taatccttat tcatggctct gggccgggcg tctcggcctg ggccaactgg caattcgcca
     4381 tccccttttt agcgcagcgg ttgcgcgtct ttgcttatga ccagcttggc ttcggctatt
     4441 ccgaagcagc gcccaatcaa tcttatagcc tgaagcgctg gactgaccat ctgctcaatt
     4501 tcatgcaggc ggtaggggtc agacgggcgc acctcgtcgg caactcgatg ggtgccgcgg
     4561 tcgctctggc tgccgccgtg acgcagcccg aggcagtcga tcgcctggtg ctgatgggcg
     4621 cgatgggcgt gcgtttcaat ctcaagcggg gggaagggtt ggatgccgtg tgggggtata
     4681 cgcccagcgt tgagaatatg cgccgcctga tcgatctttt tgcctacaat ccaggcaggt
     4741 tcccaaccga gcagctggcg aaactgcgct acgaagccag catccggcca ggagcgcaag
     4801 aggcattctc cagcatgttc cctgagccgc gccagaaaca agtggaagcg ctggccgcct
     4861 acgaggatca cctctccgat gtgcagaatc ccacgcttat cattcatggg cgcgaagacc
     4921 gcgttatacc aattgagaca agccttaaat tgatcaacac gctgcccaac gcgcagttgc
     4981 acgtcttcgg tcactgcggc cattggacac agctcgaacc tcgcgacacc ttcaaccgtc
     5041 tggtgcgaga tttcctgacc gaggaaacgt ctggttaatg tgggggagaa atagagcaag
     5101 cagcaagaaa accagggcga tggccagcat cgcccctacc ctctctacac cggcaaggga
     5161 agcgttgtga gggtaggggc gatgctggcc atcgccccat aggcgtcaag ttaagccgga
     5221 cgcccaagcg ctctgtaaga ggcgggaggt actcaaccga gtactccg
//
LOCUS       DASZAK010000049         3544 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_568984, whole genome shotgun sequence.
ACCESSION   DASZAK010000049 DASZAK010000000
VERSION     DASZAK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3544
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568984"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /gene="infB"
                     /locus_tag="VGT44_00805"
     CDS             <1..1782
                     /gene="infB"
                     /locus_tag="VGT44_00805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV2579362.1"
                     /translation="PISLPAQIVVKDLADLLHATPNEVISDLIKEHGIFANINQVIDY
                     DKASLVAQDLGFEPSQTAAAVAPAVQRQRESVVPSPVVEEEQREQEQLVVIPPVVTIM
                     GHVDHGKTSLLDVIRKTKVAAGEAGGITQHIGAYQVEVQGKKITFLDTPGHEAFTAMR
                     ARGAQVTHIAVIVVAADDGVMPQTREAIDHARAAKVPIIIALNKMDKLDANPDYVKQQ
                     LAEIDVVVEDYGGDVICVPVSARKGTGIDDLLEMILLVAEIQDIRANPNQLATGTIIE
                     ARLEKNSGPTATVLVANGTLKMGDNVVVGSMAGKVRAMFNDRGKRIQKAPPSTPVSIL
                     GLPEVPRAGDRLEAVADERTARQLATQTAEQRRGETRSQGQVSLDTLYSQMQQGSVKE
                     LNIVLKCDVQGSAEAIKHSLSKVGDEGLKVRLIHEGIGNISETDVHLAGASDAIIIGF
                     NVKADGASQRLAQEEGVDIRYYNIIYKLLDDIEAALTGMLEPTYREVIDGHAEIQQTF
                     KAGKNIVIAGCRILDGKITRSSQARILRKKERIWDGKIASLRRGKDDVREVSSGYECG
                     IILEEFSDFEVGDIIEAYSQEKVRPGA"
     gene            1813..2190
                     /gene="rbfA"
                     /locus_tag="VGT44_00810"
     CDS             1813..2190
                     /gene="rbfA"
                     /locus_tag="VGT44_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV2579363.1"
                     /translation="MPNPHRQEKLSELIAAELSELLRTRVKDPRVGFVSITRVEVSGD
                     YRHAKVYTSVMGNKDEQTGTLRALQHASGFLRHELAGRLDLRFTPEIIFKLDHSIEEG
                     ARILDLIREVEREQAIDEGSNHE"
     gene            2183..3241
                     /locus_tag="VGT44_00815"
     CDS             2183..3241
                     /locus_tag="VGT44_00815"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV2579364.1"
                     /translation="MSEEAPSGACGLDAQLDQRLVQAALALIEPARHILLIAHEHPDG
                     DCIGSALGLARILELAGKSCVPACADPAPGNLSFLPGIQALRQTLDDEEFDLVIALDA
                     GEFYRFGALYETHRAFFDNALVLNLDHHISSEGCGQINIIDPGSAATAELLVLFQQQA
                     RLALDRDAALCLLTGLITDTNSFQFTNTTPRCLEVAALLLRAGAIPETVVQPIYRTKP
                     LAQVRFQSMILASAQTACEGRLIWAQATGTTLAAAGATPDMDENTSGMLRDIEGVQVA
                     AYFKSYGDPTITRLSLRCAKPYNAADICQRLSSSIGNGQGGGHPRAAGATFHLPIAQT
                     VEYVIAELMKEMQTTSYG"
     gene            3251..>3544
                     /locus_tag="VGT44_00820"
     CDS             3251..>3544
                     /locus_tag="VGT44_00820"
                     /inference="COORDINATES: protein motif:HMM:NF013660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579365.1"
                     /translation="MDGILNINKPTGMTSHDVVARVRKLLAQRRVGHAGTLDPAASGV
                     LPICVGQATRVAEYLSESGKAYRAAILFGIETDTYDADGAVTETASTTTLSLAH"
BASE COUNT          799 a         1073 c         1059 g          613 t
ORIGIN      
        1 ccgatctcgt taccggctca gatcgtcgta aaagacctcg cggatctgct gcatgccaca
       61 ccgaacgagg tcatcagcga tctgattaaa gagcacggca tctttgccaa tatcaaccag
      121 gtcattgact acgataaggc atcccttgtg gcgcaggatc tcggatttga gccgagccag
      181 accgcagccg ccgtcgcgcc cgccgttcag cgccagcgcg agagcgttgt cccatccccg
      241 gtagtcgaag aagagcagcg cgagcaagaa caactggttg tgattccccc agtcgttact
      301 attatgggac acgtcgatca cggcaagact tcactgctgg atgtgatccg taaaacgaag
      361 gtggcggcgg gcgaggcagg cggtattacg caacacattg gcgcgtacca ggtggaagtt
      421 cagggtaaaa agatcacctt cctggataca ccgggtcacg aggcgttcac cgccatgcgc
      481 gctcgtggag cgcaggtgac gcacatcgcc gtgattgtgg tggccgcaga cgacggcgtg
      541 atgccgcaga cgcgcgaggc tatcgaccat gcgcgggcag ccaaagttcc catcatcatc
      601 gcgctcaaca agatggacaa actggacgcc aatcccgact acgtgaagca gcagctcgcg
      661 gaaatcgacg tggtggtcga ggattacggc ggcgacgtaa tttgcgtacc cgtttcggcg
      721 cgcaaaggca ctggcatcga tgatctgctg gagatgatcc tgctcgtcgc cgaaatccag
      781 gatattcgtg ccaaccccaa ccagcttgcc accggcacga tcattgaggc acgcctggag
      841 aagaacagcg gcccgacggc aacggtactg gtagcaaacg gcacgctcaa gatgggcgat
      901 aacgtggttg tcgggtcgat ggccggcaaa gtgcgtgcta tgttcaacga tcgcggcaaa
      961 cgcatccaga aggctccacc cagcaccccc gtcagcatcc tgggtttgcc cgaagtgcca
     1021 cgcgccggtg atcgcctgga agcggtcgcc gacgagcgta ccgcccggca acttgccacg
     1081 cagaccgccg aacagcggcg cggcgagact cgctcgcagg gacaggtcag cctggacacg
     1141 ctctattcgc agatgcagca gggcagcgtc aaagagctga acatcgtcct caaatgcgac
     1201 gtgcagggtt cggctgaagc aatcaagcat tcgctctcca aagtcggcga cgagggactc
     1261 aaagtgcgcc tgattcacga gggcatcggc aatatcagtg agactgatgt tcacctggcg
     1321 ggggcgtccg acgcgatcat cattggcttc aacgtcaaag ccgatggcgc ctcgcagcga
     1381 ctggcccagg aagaaggcgt agatatccgc tactacaata tcatctataa gcttctcgat
     1441 gatatcgaag cggccctgac cggtatgttg gaaccaactt atcgcgaagt gattgatggc
     1501 cacgccgaga ttcagcagac cttcaaagcg ggcaagaaca tcgtgatcgc gggctgtcgc
     1561 atcctcgacg gcaaaatcac gcgctcttcc caggcacgta tcctgcgcaa gaaagagcgg
     1621 atctgggatg gcaagatcgc ctcgctgcga cgcggcaagg acgatgtgcg cgaggtgtca
     1681 agcggctacg agtgcggcat tatccttgaa gaattcagcg acttcgaggt cggcgacatc
     1741 atcgaagcct attcgcaaga gaaggtcagg ccaggagcat aatccattag ttgtaggggc
     1801 agcgaaagaa tcatgccaaa tccacataga caagagaagc tcagcgagtt gatcgcagcc
     1861 gagctgagcg agttgttgcg cacgcgcgtg aaggacccgc gtgtcgggtt cgtcagcatc
     1921 acgcgcgtcg aggtgagcgg cgactaccgg cacgcgaagg tctacaccag cgtgatggga
     1981 aataaggacg agcaaaccgg cacactgcgc gcattgcagc atgcgagcgg tttcttgcgc
     2041 cacgaactgg ccggtcggct cgacctgcgc tttacacctg agatcatctt taaactcgac
     2101 cattctatcg aagagggtgc gcgcatcctc gacctcatcc gggaagtgga aagggagcag
     2161 gccattgacg aaggaagcaa tcatgagtga ggaagctcca agtggcgcat gtgggctgga
     2221 tgcgcagttg gatcagcggc tcgtgcaagc cgccctggcg ctgatcgaac ctgcgcggca
     2281 catcctgttg atcgcgcacg aacaccccga cggcgattgc attggctcgg cgctcggcct
     2341 ggcgcgcatc ctggaactgg cgggcaaaag ctgcgttccc gcctgcgccg accccgcgcc
     2401 cggaaacctg tcgttcctac ccggcattca ggcattgcgg caaacgctcg atgacgaaga
     2461 gttcgacctg gtcatcgccc tggatgccgg ggaattctac cgtttcggcg cgctctacga
     2521 aacgcatcgc gccttctttg acaacgcgct cgtgctgaac ctggatcacc atatcagcag
     2581 cgaaggctgc gggcagatca atatcatcga tcccggctcg gcggcgaccg ccgagctgct
     2641 cgtgttgttc caacagcagg cgcgcctggc gctcgaccgc gatgcggcgc tgtgcctgct
     2701 gacgggtctg attaccgata ccaactcgtt ccagttcacc aacacaaccc cgcgctgcct
     2761 ggaagtggcg gcgctgctgc tgcgcgcggg cgctattccc gaaacagtcg tgcagcccat
     2821 ctatcgcaca aagccactgg cgcaggtgcg cttccagtct atgattctcg ccagcgcgca
     2881 gaccgcttgc gaaggccgcc tgatctgggc gcaggcaacc ggtaccacgc tcgccgccgc
     2941 gggcgcgaca ccggacatgg acgaaaacac ttctggcatg ctgcgtgata tcgaaggcgt
     3001 acaggtagca gcgtacttca aaagttacgg cgacccaacc attacgcgtc tgagcctgcg
     3061 ctgcgccaaa ccctacaacg ctgcagacat ctgccagcgc cttagcagca gcatcggcaa
     3121 cggccagggc ggcggccacc ctcgcgccgc cggagccacc ttccacctac ccatcgcgca
     3181 gacggtggag tacgtgattg cggagctaat gaaagagatg cagacaacct cgtatggata
     3241 gcaaataagc atggatggca ttctcaacat caataaacca accggcatga cttcacacga
     3301 tgtcgtggcc agagtgcgca agctgctcgc gcagcggcgc gtcggccacg cggggacgct
     3361 cgacccggca gcgagcggtg tactacccat ctgcgtcggg caggcgactc gcgtcgcgga
     3421 atatttaagc gagagtggca aagcctatcg cgccgccatc ctcttcggca tagagaccga
     3481 cacatacgat gccgacggag cggttacaga gacggccagc acaaccaccc tctctctggc
     3541 gcac
//
LOCUS       DASZAK010000050         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_107714, whole genome shotgun sequence.
ACCESSION   DASZAK010000050 DASZAK010000000
VERSION     DASZAK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107714"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VGT44_00825"
     CDS             <1..805
                     /locus_tag="VGT44_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579366.1"
                     /translation="HAMSNFSFALRDSATMLRRDIQHSLRRLSMTLSGLLVPIIMLVL
                     FVYVFGGAIGASLGGMALGGSYINFVAPGIIIMTVGSGCATTAINLCMDMSEGIIARF
                     RTMAISRSSVLTGQVLGSLIRTMLGVCLIIGVALLMGFRPTANPVAWLAALGLIALLT
                     LAITWMGVAFGLVGKTPAGANSLSLIFQLLAFTSSAFVRPDSMAGGVRWFAEYQPFSP
                     VIDTLRGLLLGTPIGGSTVALAVAWCVCLTLLGYLWARTAYNRSSVSAS"
     gene            879..2369
                     /gene="mtaB"
                     /locus_tag="VGT44_00830"
     CDS             879..2369
                     /gene="mtaB"
                     /locus_tag="VGT44_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HEV2579367.1"
                     /translation="MSNQLSSTPTFAVATLGCKVNQADSEAICEQMCAAGFAQRDFGE
                     QADIYIVNTCTVTHLGDRSSRQMISQARRRHPGALMVVTGCYAEQNPQAVASLPGVDL
                     VVGNRGKETLVEAIQEQWTISGRTSVTDGIGERKLSAREQVASVASAANAESAAARLL
                     PVLRLDSQHIGSDDEPQPDNPAQVALLIDAVDHSSANAPLYTRTRVQMKVQDGCDNRC
                     TYCIVPYVRGGSRSRSIASVVEHVQRKAHAGFKEVVLTGIHLGDYHPGDDERLDLGDL
                     IDALLHETEIPRVRVSSLEPEDFRLEWLELWQNPRMCRHLHPPMQSGSDAILRRMARR
                     YLSERYRAIVTTAKQRVPGIAISTDIITGFPGESDEDFERTYQLAAELQFAKAHVFRF
                     SPRQGTPAARMRGQIKDELKKARSERLLALNEQDMQRFRQQFLGETVEVLIEAHKHGR
                     WEGLTDNYLRVELEGLPDANRDWQNTLVKARLVNLVDDGVLGVFNT"
BASE COUNT          466 a          769 c          752 g          480 t
ORIGIN      
        1 ccatgcgatg agtaattttt ccttcgcctt gcgcgattcg gcgaccatgt tgcggcgcga
       61 tatccagcac tcgctgcgcc gcctctcgat gacgctctct ggcttgctgg tgccgattat
      121 catgctagtg ctgttcgtgt atgtgttcgg cggcgcgatt ggcgccagtc tcggcggcat
      181 ggcgcttgga ggcagctaca tcaacttcgt cgcgccaggg atcatcatca tgaccgtggg
      241 cagtgggtgc gcgacgacgg caatcaacct ctgcatggac atgagcgaag gcatcatcgc
      301 ccgcttccgc accatggcga tctctcgctc ttcggtgctg accgggcagg tcctcggcag
      361 cctgatccgc acgatgctcg gcgtctgcct catcatcggg gtcgcgctgc tcatgggctt
      421 ccggcccacg gcgaatcctg tcgcctggct cgcggcgctc ggcctcatcg cgctgctcac
      481 cctcgcgatc acgtggatgg gggtggcgtt cggccttgtg ggcaaaacgc ccgcgggagc
      541 taatagcctc tccctcatct tccagctcct ggcgttcacc agcagtgcgt ttgtccgccc
      601 ggactcgatg gcgggcgggg tgcgctggtt cgccgaatac cagcccttct cgcccgtcat
      661 cgacacgctg cggggactgc tgctgggtac gccgatcggc ggtagcaccg tcgcccttgc
      721 agtcgcctgg tgcgtctgcc tcactctgct cggctacctg tgggcgcgaa cggcctacaa
      781 ccgcagctcc gtatcggcta gttgaaggct atgatacaat tgtcttcgat aactgcgggc
      841 gcaattgagg gaagaggatt atcgaagaaa caactatcat gtccaatcaa ctatcatcga
      901 caccaacctt cgcggtggcg acgctgggct gcaaggtcaa ccaggccgac agcgaggcca
      961 tttgcgagca gatgtgcgcg gcgggcttcg cgcagcgcga cttcggcgag caggccgaca
     1021 tctacatcgt gaatacctgc accgtcacgc atctgggcga ccgcagctcg cggcagatga
     1081 tctcccaggc gcggcggcgg catcctggcg cgctgatggt cgtgacgggc tgctatgccg
     1141 aacagaaccc ccaggccgtt gcctcgcttc ccggcgttga tctcgtcgtg ggcaatcgcg
     1201 gcaaagaaac gctcgtggag gctatccagg agcagtggac tattagcgga agaaccagtg
     1261 tcacggatgg gattggcgaa cgaaagcttt ctgcacgtga acaggtcgca agcgtagcaa
     1321 gcgctgcaaa cgcggagagc gctgccgctc gcctgctgcc ggtgctgcgc ctggacagcc
     1381 agcatattgg cagcgacgac gagccgcagc cggacaatcc tgcccaggtt gcgctgctta
     1441 tcgatgctgt cgatcattcc tcagccaatg cgcccctgta tacacgcacg cgcgtgcaga
     1501 tgaaagtgca ggatggctgc gacaatcgct gcacctattg cattgtgccg tatgtacgcg
     1561 gcgggtcgcg cagccgcagc atcgcctcgg tggtggagca tgtacagcgc aaagcacacg
     1621 cgggcttcaa agaagtcgtg ctgacgggca ttcacctggg cgactatcat cccggcgacg
     1681 atgagcggtt agacctgggg gatttgatcg atgccctgtt gcacgagacg gaaatacccc
     1741 gtgtgcgcgt ctcctcgctc gaaccggagg attttcgcct ggaatggttg gagttgtggc
     1801 agaacccgcg catgtgccga cacctgcacc cgcccatgca gagcggctcc gacgccatcc
     1861 tgcggcgcat ggcgcgccgc tatctcagcg aacgctatcg cgccatcgtc acgaccgcca
     1921 aacagcgcgt ccccggcatc gcgatcagca ccgatatcat caccggcttc cccggcgaaa
     1981 gtgacgagga tttcgagcgc acctaccagc tcgcggcaga attgcagttc gctaaagcgc
     2041 acgtctttcg cttctcaccc cgccagggaa cgcccgcggc ccggatgcgc gggcagatca
     2101 aagacgagct aaagaaagct cgcagcgagc gtctgcttgc cctcaacgag caagatatgc
     2161 agcgtttccg ccagcaattt ctgggagaga cggtcgaagt cctcatcgaa gcccacaaac
     2221 acgggcgctg ggagggcctg accgacaact acctgcgcgt cgaactggaa gggttgccag
     2281 acgccaaccg tgactggcaa aatacgctcg tcaaagcccg actcgtgaat ctggtagatg
     2341 atggcgtcct gggtgttttc aatacctgat gaaataccgt cgtagggatg cgatgatgta
     2401 gtttaaatac atagcggggt attcctgtag gtgccggttc accgcgcaca ccgccgattc
     2461 atcggcc
//
LOCUS       DASZAK010000051         3068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_462655, whole genome shotgun sequence.
ACCESSION   DASZAK010000051 DASZAK010000000
VERSION     DASZAK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462655"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(318..1091)
                     /locus_tag="VGT44_00835"
     CDS             complement(318..1091)
                     /locus_tag="VGT44_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HEV2579368.1"
                     /translation="MPLLEGKKALIAGIANHRSIGWAIAQALAREGAQLVFTYQDRME
                     KYVKELAEKIPGTPLILCDVQDDVQIDSAFAQVGELFDGKLDIFIHSVAFAPPQELEN
                     PFVQTSRAGFHTALDISAYSLVATARRAMPLMQANGGGSIVTLTYMASERVMPKYNVM
                     AVAKAALECSVRYLAFELGEFNIRVNAISAGPLNTLAARGVSGFTSFRGHVEAAAPLR
                     RNIEQSDVGDTALFLCSPLARSITGEVLFVDAGYNIMGA"
     gene            complement(1108..1458)
                     /locus_tag="VGT44_00840"
     CDS             complement(1108..1458)
                     /locus_tag="VGT44_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2579369.1"
                     /translation="MPEYEEEEVPYATIGTDDAKRMIEAGARVIDVRQPEEWNRGHIA
                     EATLVPINGIYSFGKALQEQQVGPDEEVIFVCASGQRSASASEIALVTGLKKVYNLAN
                     GMNGWMMRRYPIER"
     gene            complement(1470..1721)
                     /locus_tag="VGT44_00845"
     CDS             complement(1470..1721)
                     /locus_tag="VGT44_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579370.1"
                     /translation="MRVVDDSQAKFSAVLRELLIWKQLPESDLSRMMPKFFLITDDFR
                     DMEDQGLLTMAFAGDEYVLCPTTYGRLWLEQYDAGQSKQ"
     gene            1910..>3068
                     /gene="hutI"
                     /locus_tag="VGT44_00850"
     CDS             1910..>3068
                     /gene="hutI"
                     /locus_tag="VGT44_00850"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEV2579371.1"
                     /translation="MMPDAFQPRVEASTVIRNIGQLVTVAQQTIPGASGPLQVIPNAA
                     IAVHNGAITWIGPDDDAEPMFQHHTETNPDEIPREIALVDAQGAVVTPGFVDSHTHLV
                     FAGDRAEEFHLRRGGVSYGELLAQGRGILTTMRATRESDTETLTGLATTRLEAMRLHG
                     TTTVEVKTGYGLDRVTEETCLHIINNLNVLESNPAYAHNHLRVVPTFLGAHSIPPEYR
                     DNRSGYIDLVVDQMLPSFTGLARFCDVFCEREAFSLEESRRILTQAKELGYLLKIHAD
                     QLSPSGGSRLAAELGAVSADHLDHASDADLDALREAGVVATLLPGCSYTLRSPYPAAR
                     RLLDRGLHVALATDFNPGTSYCENMQTIIGLAMSNMGMSLEEALSAATINGA"
BASE COUNT          642 a          950 c          838 g          638 t
ORIGIN      
        1 tgcgcatcgc cgatttatcg gccactggag cgcgttgata tatcctgcct gtcgccgatg
       61 atgaaggctc atgaccaaag ccagtcatgt catcctgagc gcagcgaagg atctgatgcg
      121 ccaaccaccg agatccttcg ctgcgctcag gatgacaact tcttaccgat cttggtcgtt
      181 caagttcttt atctcgtccc tacgagggta cgcccttacc atgactacgc aaccatcaag
      241 cactcgacgc atcacggtaa gggcgtaccc ttgtggtcgc cctctcggat tccaggatgg
      301 tacacccttc aaactactta tgcccccatg atgttatacc cggcatccac aaagagaact
      361 tctccggtga tggagcgtgc caggggactg cacaggaaca gcgcagtgtc gcctacatcg
      421 ctctgctcga tattgcggcg taggggagcc gccgcttcga catggccgcg gaaggacgtg
      481 aagccggaga cgccgcgcgc cgccagcgta ttgagcggcc cggcggagat ggcgttgacg
      541 cggatgttga actcgcccag ttcaaatgcc aggtagcgca cgctgcattc cagtgcagcc
      601 ttcgcgacgg ccatgacgtt atacttgggc atgacgcgct cgcttgccat gtaggtcagc
      661 gtgacgatgc tgccgccgcc attggcctgc atcagcggca tggcgcgtcg cgccgtggca
      721 accagcgagt aggcgctgat gtccagcgcg gtgtggaatc cggcgcgact ggtctgcaca
      781 aagggatttt ccagttcctg cggcggggca aaggccaccg agtgaatgaa aatatcgagc
      841 ttaccatcga agagttcgcc gacctgcgcg aaggccgagt cgatctgcac gtcatcctgc
      901 acgtcgcaga ggatcaaggg agtgcctgga atcttttcgg ccagctcctt gacatacttc
      961 tccatgcggt cctggtaggt gaagaccagt tgcgcgcctt cgcgtgccag cgcctgcgcg
     1021 atagcccagc ctatcgagcg gtggttggca atacccgcga tcagcgcttt ctttccttcg
     1081 agcaatggca taggtgtact cctctcatta tcgttcaatc gggtaacgcc gcatcatcca
     1141 tccgttcatc ccgttcgcca ggttgtagac cttcttcagg cccgtcacca gcgcgatttc
     1201 agaggccgag gcgctgcgct gtccgctggc acagacgaag atcacctctt catcgggtcc
     1261 aacttgctgc tcctgcaacg ccttgccgaa gctatagatg ccgttgatcg gcactaacgt
     1321 agcctcggcg atatggccgc gattccactc ttccggctgc cgcacatcga tgacacgcgc
     1381 gcccgcctcg atcatgcgct ttgcatcatc agtgccaatc gtggcatatg gaacctcttc
     1441 ttcctcatat tctggcattg atcttctcct tattgtttgg attgtccggc gtcgtactgc
     1501 tccagccaca gtcggccata ggtggtagga cacaggacat attcatcgcc ggcaaacgcc
     1561 atggtcagca acccctgatc ttccatgtct cgaaagtcat cggtaatcag gaagaatttg
     1621 ggcatcatgc gcgaaagatc gctttcgggc aattgcttcc atatcagcaa ttcgcgcaga
     1681 accgccgaga atttcgcctg gctatcgtca actactcgca taagcgcctc tctctccgta
     1741 tttctgacca cagtgtacca aatacaataa caatgagcaa gttccagcga gagaaccaca
     1801 gggcgatgct ggccatcgcc ctggttctct tcgccccggc actctcgaca caagctttcc
     1861 tgtgtgttat aacagggaaa agtacactcc acactaagga gcaacctata tgatgcccga
     1921 tgcatttcaa ccgcgcgtcg aagcaagcac agtcatacgc aacatcggcc aactggtcac
     1981 agtggcgcag caaacgatcc caggcgcaag tggcccactg caggtcatcc cgaacgctgc
     2041 cattgcagtg cataacgggg cgattacctg gattggaccc gacgacgatg cagaaccaat
     2101 gttccagcat cacacggaaa ctaacccgga cgagatacct cgtgaaattg cgctggtgga
     2161 tgcgcaggga gcggttgtca cgccggggtt cgtcgattcg catacccacc tggtcttcgc
     2221 gggcgaccgc gccgaagaat ttcatctgcg gcgcggcggc gtgagctacg gcgaattgct
     2281 ggcgcagggg cgcggcatct tgacgaccat gcgcgccacg cgcgagtcgg atacagagac
     2341 gctgacggga ctggcaacca cgcgattgga agcgatgcga ctgcacggca cgacgacggt
     2401 ggaggtgaag accggctatg ggctggatag agtcactgaa gaaacgtgcc tccatatcat
     2461 caataacctc aatgtcctag aaagcaatcc ggcctacgcg cataaccacc tgcgcgtggt
     2521 tcccaccttc ctcggcgcgc acagcatccc gcccgaatac cgcgacaacc gttcaggcta
     2581 tatcgatctc gtcgttgatc agatgctgcc ctccttcacc ggactggcgc gtttctgcga
     2641 tgtcttctgt gagcgtgaag cgttctcact cgaggagagc cgccgcatct tgacacaagc
     2701 gaaagaactg ggctacctgt tgaagatcca cgccgatcaa ctcagcccat cgggcggctc
     2761 gcgcctcgcc gccgaactcg gcgcggtttc cgccgaccac ctcgatcatg ccagcgatgc
     2821 cgacctcgac gccctacgcg aggcaggtgt tgtcgcgacg ttgctacctg gctgctccta
     2881 caccctgcgc tcgccctatc cggcggcgag acgcctgctg gatcgcggcc tgcatgtcgc
     2941 gctggcgacc gactttaatc ccggtacatc gtactgtgaa aatatgcaga ccatcatcgg
     3001 cctggcgatg tccaatatgg gtatgtcgct tgaagaagca ttgtccgcag ccaccatcaa
     3061 cggggcgc
//
LOCUS       DASZAK010000052         2014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_1099, whole genome shotgun sequence.
ACCESSION   DASZAK010000052 DASZAK010000000
VERSION     DASZAK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..>2014
                     /locus_tag="VGT44_00855"
     CDS             297..>2014
                     /locus_tag="VGT44_00855"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2579372.1"
                     /translation="MFDKLFQPITIGSHEAKNRIIFGSHTTNFARNNLLSQQHADYYA
                     ARAEGGAGVVLLEEHIVHPSDIPYEYALAGYLPETVEAMRHVTQRIHAHGALAIVQLN
                     HNGQQSVSDHTQRELWAPSAVPDVSSREVPKAMQARDIAAVIDGFALVARHAAQGQAD
                     GVELQIADRSLLRQFLSPLTNQRGDAYGGSLENRLRFVQETIEAVDAALRSEHILGVR
                     LCVDELAPWAGITPEQGIDIACLLAETGRIDYLTITMGSIYSIHMFPFHASMHIPRGY
                     AVHLAAAVKAVIPPTIPVFAAGRIMDPRQAEAILAEGQADGVEMIRALIADPDLPRLS
                     RAGQPGSVRPCISCNQGCQVRNVMNAAIACNVNPDVMRNMHPSTGTTYIEQPPGRDDE
                     GSQTRPVRDCHPSLRSGSQWMDAEMLRYAQHDNTGVGRETSSARPNDRQRSYLVVGGG
                     PAGMEAARTLALRGRRVTLYEREAALGGAVTLAAGAPGRGELRLIVEYLQRQLEQLDV
                     TIRLGVEVTAEMILAERPEAVIVATGARSGSGLLPIPGSDLPHVTDMRRVLRGEQATG
                     QRIVVL"
BASE COUNT          421 a          633 c          622 g          338 t
ORIGIN      
        1 ctggaggatc tgctccggag gcatggatat cggcagatac aaacgaaagc ctccacacta
       61 gagtctcggg cgggaaccac tgaggggcag gcattgtacg aagatatggt tcattcattt
      121 caagcgatac gaccgtttat ccaaaagtgg ggctgtgatg atgccggaaa atatgatctc
      181 ctctgtcaac aagccctggg tgaaatacaa cagagcggtt cttacgcgac ctggaagttc
      241 ctcacagcat ggggaaacaa acgctgacct caatccaaac atggtaaaat atccccatgt
      301 tcgacaaact cttccagcca atcacaatcg gctcacatga ggccaaaaat cgcataattt
      361 tcggcagcca taccaccaac tttgcgcgca ataacctgct cagccagcag cacgctgact
      421 attacgcggc gcgcgccgag ggcggagctg gcgtcgtatt gctggaagag catatcgtac
      481 atccctcgga tataccttac gaatacgccc tggccgggta tctgccggaa acggtagagg
      541 ctatgcggca tgtgacgcag cgcattcacg cgcacggggc gctggctatc gtccaactca
      601 accacaatgg gcagcagtcg gtgagcgacc acacgcagcg cgaactctgg gcgccctccg
      661 ccgttcccga tgtctcctcg cgcgaagtgc caaaggctat gcaagcgcgc gacatcgcgg
      721 cggtgatcga cggcttcgcc ctggtcgccc gccacgccgc gcagggccag gccgacggcg
      781 tcgagttgca gattgccgac cgctcgctgc tgcgccagtt cctctcgcct ctgacgaacc
      841 agcgcggcga tgcctacggc ggctcgctcg aaaaccggct gcgcttcgtg caggagacca
      901 tcgaagcggt tgacgccgcg cttaggagcg aacacattct cggcgtgcgg ctgtgcgtgg
      961 atgaactcgc gccctgggcg ggcatcacgc cggagcaggg catcgacatc gcgtgcctgc
     1021 tggccgagac ggggcgcatc gactacctga cgataacgat gggcagcatc tatagcattc
     1081 acatgttccc cttccacgcc tccatgcaca tcccgcgcgg ctatgccgtc cacctggcgg
     1141 cggcggtcaa ggccgtcata ccacccacca tacccgtttt cgcagcggga cgcatcatgg
     1201 acccgcggca ggccgaggcc atcctggcgg aaggacaggc cgacggggta gaaatgatcc
     1261 gcgccctgat cgccgacccc gatctgcccc gcctctcgcg cgcagggcag cccggcagcg
     1321 tccgcccctg catctcctgc aaccagggct gccaggtgcg caacgtcatg aacgccgcca
     1381 tcgcctgcaa cgtcaacccc gacgtaatgc gcaacatgca cccctcgacc ggcacaacat
     1441 atatcgagca accaccagga cgcgatgatg aaggttccca aacaagaccg gtcagggatt
     1501 gtcacccttc gctgcgctca gggtctcagt ggatggacgc cgagatgctt cgctacgctc
     1561 agcatgacaa caccggcgtt ggtcgtgaaa cttcatcagc gcgtcccaac gacagacaga
     1621 ggtcatatct cgtggtcggc ggcggaccgg cgggaatgga ggcggcgagg accctggcgt
     1681 tgcgcgggcg gagggtgacg ttgtacgaac gcgaggcggc gctgggcggc gcggtaacgc
     1741 tggcggcagg cgcgccggga cgcggcgagc tgcggctgat cgttgagtac ttgcagcgac
     1801 aactggaaca actggatgta acgatacgcc tgggcgtgga ggtgacagcc gaaatgatcc
     1861 tggcggaacg gccagaggcg gtcatcgtcg cgacgggagc gcgcagcggg agcggtctgc
     1921 tgcctatccc tggctctgat ctgccgcacg tgaccgatat gcggcgcgtc ctgcgcggcg
     1981 agcaggcgac gggacagcgt attgtggtac ttga
//
LOCUS       DASZAK010000053         5322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_320689, whole genome shotgun sequence.
ACCESSION   DASZAK010000053 DASZAK010000000
VERSION     DASZAK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320689"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..3428
                     /locus_tag="VGT44_00860"
     CDS             63..3428
                     /locus_tag="VGT44_00860"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2579373.1"
                     /translation="MIDSLTIHLLGTPEARLAGTPLTLHHQKARALLYYLAATGRPFT
                     RDHLASLLWSETPENNARHSLRSSLYHMRQALHAQGADEALVGDGDMISLRLNDDACD
                     VLTFRRLFAQNSEQALARAVTLYHGPFLQGFTVTDAPLFEEWLRFEQDELAQVYLTMS
                     QRLASWAEQRQDWSEVIANLQRIVQVEPLSEEIQQRLMQAYLRTGAIGQALRQYHQFE
                     AELKHELDLTPSLETQALFSSALLMRQNPPTQSEKVSRTPDLHQRALRELPFVGRDEV
                     FHQLAAISKQVSAGRGCAVLIQGEDGVGRSRLLYELASVLEANQPRWMILQGSCSPFD
                     DLLSYGPFLEAFQQANLGDLLSQARDIDASEQQRFQWVFLQALQTLTRGAPLLLSIDD
                     LQWANSQTLHLFGFLATRLRALPVLLVGTVQRAEAIPALQRLVTLGRRHGDVHTLSLL
                     PLTIEDVRTVTTNLGIGSAPDTWTSETISEWLYERSGGNPFLISEIMAQLQADGILST
                     FEGRMRLDVGRWRRWRATFNLPETTHDLVAWRLTNLSPQARALLDVLAVANLPLPFIL
                     LREFPGLSGADPSQSAGEQLLATVEDLVARGLVVETGHELLTLPHNLLCETLQHQLSR
                     LRRVEIHRQLAKIIEHCPVLQAHFPLRQLALHAVAGEDFERARRYGLQILDELVQDNA
                     NAQTAVFLRHLHDLLAQADAPYDRLRLTLALGKVHQSLGRLEEAGDWHRQHLDLARQV
                     GDLSASAAGHYELGELALVANDYQAAGSEARTGLAVELPADHPQRLALTARGYWLLGA
                     ALAMEGNDLPSAQHYLLEAIDIHRLTDNVSDLCATLFELGNVAAQGGELEHALERYQE
                     AERIAELAHVHYIHALANNNFAYHSLLLGRPHDARQALTAGTKLAERHELFGALMHLS
                     STQGELHLYLGEWRAAEEAFQNSLALAEELRNLERQAGNRAGLALAARGQGDIESTLK
                     LLEEALQLITERGFWHLRTRIQLWLAETLLPDSQAHRTEQAAHYLEAALATARTHARQ
                     LLLLQGERLQARLLATREEGPAAQALFAQALDRATDLHLPLETARTKAAWGQSLLRYA
                     PREGQRLLSEARQELAAHGAQADLHTIEGAVRV"
     gene            3531..>5322
                     /locus_tag="VGT44_00865"
     CDS             3531..>5322
                     /locus_tag="VGT44_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2579374.1"
                     /translation="MIGPIISGRWGRNSEDKFSFKQIPGTFASLPRVVRLVWSTSPLL
                     TLLLGLLNLAQGFVPALSVSITALVIDSVVLAIRIHNASPIWLPIGLQLGITLFSSLL
                     STFSNIAQQLMQEQVSNRVQLDILKKASTLDLAFFENPEFYDKMRQAANQSLYQPVSM
                     ISQTFDLGRTTVTLISLLFLLLHLAWWLAIIALVVPIPAFFSSTRYGWRGYQLMRRQS
                     PERRLMAYFVTLMTTDIYNKEIKLFNLSRFFTDRFWELATRLYQQDKRLLVRRYSINL
                     LWTGLTVVANSAIYLYVALQAVAGRITLGGLTLYTQTAVQVGQNFQGLLNDISSTYEN
                     TLYINTLFEFLEYEPKIVSPPEPHPVDPPGEVDGLDIEFRDVSFTYPGKNPETEAALK
                     HVSFTIQPGEAVALVGRNGAGKTTLVKLLTRLYDPDEGEIFIGGRNVKEYDLAELREQ
                     VGVIFQDYVTYYMTAHDNIGVGRVEQIEERELVEIAARKSGASEVIERLPKGYETMLG
                     RWFKDLKESTQLSGGEWQKVALARAFMRDARILVLDEPTSSLDAQAEYEIFTRFRELT
                     RGKTAVFISHRFSTVRLADRIFVIEKGNLIE"
BASE COUNT         1113 a         1626 c         1537 g         1046 t
ORIGIN      
        1 caatgaattg cgcccctaca cgccacttca acctggacgg tcttgtagga gccggtaata
       61 caatgataga ttcgctcacg atacacctgc ttggtacgcc tgaagcgcgc ctggcgggaa
      121 ctccactgac gctccatcac cagaaggcgc gggcgctgtt gtattacctg gctgctaccg
      181 ggcgtccttt cacacgcgac cacctggctt ccctcttatg gagcgaaacg cctgagaaca
      241 acgcgcggca ctcgctgcgc tcaagccttt accatatgcg ccaggcgctg cacgctcaag
      301 gggctgacga ggcgctggtt ggcgacggcg atatgatctc cctgcgcctc aatgatgatg
      361 cgtgtgatgt gctgacgttc cgccgcctgt tcgcgcaaaa cagcgaacaa gcgctcgcgc
      421 gcgcagtgac gctctatcat ggcccgttcc ttcaaggctt tactgtgacc gatgcacccc
      481 tgttcgagga atggctacgt ttcgagcagg atgagctggc gcaggtgtac ctcaccatgt
      541 cgcagcggct ggcctcctgg gcagaacaac gccaggattg gagcgaggta attgcgaatc
      601 tacagcgcat cgtccaggtc gaaccgctgt ccgaagagat acagcagcga ctaatgcagg
      661 cgtacctgcg cacgggagcg atcggacagg cgctgcgcca gtatcaccag ttcgaggccg
      721 agttaaagca cgaactcgac ctcactccct ctctagaaac gcaggcatta ttctcgagcg
      781 cgttgctcat gcggcagaac cctccgacgc aaagcgagaa ggtctcgcga acaccggacc
      841 tgcaccagcg cgcactgcgc gaactcccgt ttgtggggcg cgatgaggta ttccatcagt
      901 tagcagcaat cagtaagcaa gtcagcgcgg gacgaggatg cgccgttctc atacaggggg
      961 aagatggcgt cggaagaagc cgcctactct acgaactggc aagcgtcctg gaagcgaacc
     1021 aaccgcgctg gatgatacta caaggctcct gctctccctt cgatgatctg ctctcctatg
     1081 gtccgttcct ggaagctttt caacaggcga acctgggcga tctgctctca caagcgcgtg
     1141 acatcgatgc aagcgagcag cagcgcttcc agtgggtctt cttgcaggcg ttgcagacgc
     1201 tcacgcgcgg cgcgccgctg ctcctgtcca ttgatgattt acaatgggcc aatagccaga
     1261 cactgcacct tttcggcttt cttgcaacgc gactgcgcgc gctacccgtc ttgttggtcg
     1321 gcaccgtcca gcgcgccgaa gctattccag cattacaacg actggtcacg ctgggccgac
     1381 gacatggcga tgtccacacg ctatcactgc tgccattgac gatcgaagat gtcagaacgg
     1441 tcacgaccaa cctgggtatt ggctctgcac ctgatacgtg gacatctgag accatttctg
     1501 aatggctata cgaaagatcg ggtggcaacc cattcctcat atcagagatc atggcgcaat
     1561 tgcaagctga cggcattctc tccacttttg agggcagaat gcggctggac gttggccggt
     1621 ggagacgctg gcgcgcaacg tttaacctgc ccgaaaccac gcatgacctg gtagcgtggc
     1681 gtttaacgaa tctgtcaccc caggcgcgcg ccctgctcga tgtgctggcg gtcgcgaacc
     1741 tgcctctccc tttcatactc ctgcgcgagt ttcccggctt gtccggcgcc gatccgtcgc
     1801 aaagcgcggg cgaacaacta ctagcgactg ttgaagacct ggtagcgcgc gggctggtgg
     1861 ttgaaacagg gcatgagctg ttgaccttac cgcacaacct gctgtgcgag acgctccagc
     1921 atcaactgag ccggttacgc cgcgtggaga tccatcgtca actggcaaaa atcatcgagc
     1981 attgccctgt cctccaggca cattttccgc tgcgccaact ggcgttacat gccgttgccg
     2041 gggaagattt cgagcgcgcg cgccgttacg gtttgcaaat tctcgacgag ttagtccagg
     2101 acaacgcgaa cgcgcagaca gccgtctttc tgcgccatct gcacgatctg ctggcgcagg
     2161 cggacgcgcc atacgacagg ctgcggctga ccctggcgtt ggggaaggta catcaatcgc
     2221 tgggacggct tgaagaggct ggggactggc accggcagca tctcgacctc gcgcgccagg
     2281 taggcgacct ctccgccagc gcagccgggc attacgagct cggcgaactc gcgcttgtcg
     2341 ccaacgacta ccaggccgcc gggtcagagg ctcggacagg acttgccgtt gaactgcctg
     2401 ccgatcaccc acagcgcctg gcgctcactg cgcgtgggta ctggctgctc ggcgctgcgc
     2461 tggcaatgga aggcaacgat ctgccatccg cgcaacatta cttgctggaa gctatcgaca
     2521 tccatcgcct gaccgataat gtgagcgatc tgtgcgcgac gctgtttgaa ctgggcaacg
     2581 tcgccgcgca gggtggcgaa ctggaacacg cgcttgaacg atatcaggaa gcggaacgca
     2641 tcgcagagct cgcgcacgtg cattatatcc acgccctcgc aaacaacaac ttcgcctatc
     2701 acagtctttt gcttggacgc ccgcacgacg cccggcaggc gttgacggcg ggcacgaaat
     2761 tagcggaacg acacgagctg ttcggcgcgt taatgcacct ttccagcacg cagggagagc
     2821 ttcacctgta tctcggcgaa tggcgcgcgg ctgaggaagc tttccaaaat agcctcgccc
     2881 tggccgaaga actgcgtaat ctcgaacgac aggcgggcaa ccgggccgga ttggcactgg
     2941 cggcgcgtgg acagggggat atcgaatcca cgctgaaatt gctcgaagag gcgcttcagc
     3001 ttatcacgga gcgagggttc tggcatttac gtactcgtat tcaactctgg ctggcggaaa
     3061 cacttctgcc agacagccag gcgcaccgca ccgagcaggc agctcactat ctagaggccg
     3121 ccctggcaac agcgcgcacc catgctcgcc agttactgct cttgcagggc gaacgtctcc
     3181 aggccaggct gctggccact cgcgaagaag ggcctgccgc gcaagccctc ttcgcccagg
     3241 cgctagaccg cgctactgac cttcatcttc ccctcgaaac agcgcgcacg aaggcagcct
     3301 gggggcaatc ccttctacgt tacgcgccgc gagaaggcca gcggctactc agcgaggccc
     3361 ggcaagaact cgctgctcat ggtgcgcaag ctgatctgca cacaattgaa ggcgcagttc
     3421 gggtataacc acattgagca tgttctcccc ataaacagta tggtatagta tggttcagca
     3481 tgtcatgcac acctcagtaa tagttatgaa cagcgaagga cactcactct atgataggac
     3541 cgataatatc agggcggtgg gggagaaatt cggaggataa atttagcttc aaacaaatcc
     3601 ccggtacatt tgctagcctg ccgcgcgtcg tgcgcctggt ctggtcgacg agtcctctgc
     3661 tgacgctcct gctggggctg ttgaatctgg ctcagggctt cgtaccggcc ttgagcgtct
     3721 cgattaccgc cctggtgatc gacagtgtgg tactcgccat ccgaatacac aacgcctctc
     3781 cgatctggct gccgatcggg ttgcaactgg ggattacgct attctccagc ctgctcagca
     3841 cgttcagcaa tatcgcgcag cagttgatgc aggagcaggt ctccaaccgt gtgcaactcg
     3901 atatcctcaa gaaagccagt acgctcgacc tggccttctt cgagaatcca gagttctatg
     3961 ataagatgcg ccaggccgcc aaccagtccc tctaccagcc ggtgtcgatg atctcacaga
     4021 ccttcgacct cggacgcacg accgtcacgc tcatctcgct gctcttccta ttgctgcacc
     4081 tggcgtggtg gctggccatc atcgcgctgg tcgtgccgat cccggccttc ttctccagca
     4141 cgcgctatgg ctggcgcggc taccagttga tgcgccgcca gtcgccggag cgccgcctga
     4201 tggcctactt cgtgacgttg atgaccaccg acatctacaa caaggaaatc aagcttttca
     4261 acctgagccg cttttttacc gatcggttct gggaactcgc cacccgactc taccagcagg
     4321 acaagaggtt gctggtgcgc cgctactcca tcaacctgct ctggacgggc ctgacggtgg
     4381 tggccaacag cgctatctat ctctatgtcg cgctccaggc ggtggcgggt cgcattaccc
     4441 tgggcggcct gaccctgtat acccagaccg ccgtgcaggt gggccagaac ttccaggggt
     4501 tgctaaacga catctccagc acctacgaaa atacgttgta catcaatacc ctcttcgagt
     4561 tccttgagta cgaaccgaag attgtttcac cgccggagcc gcatccggtc gatccgcctg
     4621 gggaggtgga cggactcgat atcgaattcc gcgatgtcag cttcacctat cccggcaaga
     4681 acccggagac cgaggcagcc ttgaagcacg tcagcttcac gatccagcca ggcgaagcgg
     4741 tggcactggt tgggcgcaac ggcgcgggca agacgaccct ggtcaagctg ctgacgcgcc
     4801 tgtacgaccc ggacgaaggg gaaatcttca tcggcgggcg caacgtcaag gaatatgacc
     4861 tggcggagtt gcgcgagcag gtgggcgtga tcttccagga ttatgtcacc tactatatga
     4921 cggcgcacga taatatcggt gtcgggcgcg tggagcagat cgaagagcgg gagctggttg
     4981 agattgccgc gcgcaaaagc ggcgcaagcg aagtgatcga gcggctgcct aagggctacg
     5041 aaacaatgct gggacgctgg ttcaaggatc tcaaggagag tacacagctc agcggcgggg
     5101 agtggcagaa agttgccctg gctcgcgcct tcatgcgcga tgcccgcatc ctcgtactgg
     5161 acgagccaac cagctcgctc gatgcgcagg ccgagtatga gatcttcact cgttttcgcg
     5221 aactgaccag gggcaagacc gccgtgttca tcagccaccg cttcagcacg gtgcgcctgg
     5281 cggatcgcat cttcgtcatc gaaaaaggca acctgatcga ga
//
LOCUS       DASZAK010000054         1553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_498132, whole genome shotgun sequence.
ACCESSION   DASZAK010000054 DASZAK010000000
VERSION     DASZAK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498132"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VGT44_00870"
     CDS             complement(<1..681)
                     /locus_tag="VGT44_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2579375.1"
                     /translation="MQPEFTDTQAPIRWGVLSAANIAVGHVIPAIMSSASEMLVAVAS
                     RDMARAQALLGHLPGVRIYGDYESLLLDPEVDAVYIPLPNSLHGEWTIRALEAGKHVL
                     CEKPLAVTAEEGHLMAETARENNLLLMEAFMYRFHPQTIWALEQTHAGRIGSIKLVRS
                     SFSFNLMNPPRPHDIRLQAALAGGSLMDIGCYNVNFCRAVYGHAQVAVAARVSAPSLD
                     DVERSTTAV"
     gene            complement(851..>1553)
                     /locus_tag="VGT44_00875"
     CDS             complement(851..>1553)
                     /locus_tag="VGT44_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2579376.1"
                     /translation="AVELAHAASGWASGTTHNRAQILYYLAENLSVREGEFAQRIVQQ
                     TGRADTTREVQAAISRLFSYAAWCDKYEGVVHRPPMRGAVLALPEAIGVIGMACPDDH
                     PLLGFISLVAPAIAMGNTVVAIPSQAAPLSATDLYQVLETSDLPAGVVNIVSGDRDAL
                     SQVLADHDDVDAMWYFGPREGCQRVELASAGNMKRTWVSYGHARDWFDIEQGEGQEFL
                     REATHVKNIWIPYGE"
BASE COUNT          285 a          493 c          483 g          292 t
ORIGIN      
        1 tacggcggtg gtcgatcgtt ctacatcatc caggctggga gccgagacgc gcgcggcaac
       61 ggcgacttgc gcatgtccat aaacggcgcg gcaaaagttg acgttgtagc agccaatatc
      121 catcagcgag ccgccggcca gggctgcctg caagcgaata tcgtgcgggc gcggcggatt
      181 catgagattg aacgagaacg aggatcgcac cagcttgata gaaccgatgc gcccggcgtg
      241 cgtctgctct aatgcccaga tggtctgcgg atgaaaccgg tacatgaagg cttccatcag
      301 cagcaggttg ttttcgcgcg cggtttccgc catcaggtgg ccttcttccg ccgtgaccgc
      361 cagcggcttc tcgcagagga catgctttcc ggcctccagc gcgcggatgg tccattcgcc
      421 atgcagacta ttcggcaggg ggatgtagac cgcatcgacc tccgggtcga gcagcaggct
      481 ctcgtaatcg ccgtagatgc gcactccggg caaatgcccc agcagcgcct gcgcgcgagc
      541 catatcacgg ctggcaacag ccaccagcat ctcgctcgcc gacgacatga tggctgggat
      601 gacgtgcccg accgcgatat tcgcggcgct caacaccccc caacgaatgg gggcctgggt
      661 atcggtgaat tccggttgca taggtgcctc tttcttctca ccataatgcc tctcgtaggt
      721 gccgattgat cggccttgtt gcctgccatc aacccccacc gaggccgatc aatcggcggt
      781 gtgcgcgatc aatcgtcacc tacggagata tccgattcat aacttaaact ccatcatgta
      841 gcccaatgac ctactcgccg tagggtatcc atatattttt gacgtgcgtc gcctcgcgca
      901 aaaactcctg tccctcgccc tgttcgatgt cgaaccaatc gcgggcatgg ccgtagctga
      961 cccacgtgcg cttcatgttg cctgccgagg ccagttcgac gcgctggcag ccttcgcgcg
     1021 ggccgaagta ccacatggcg tccacgtcgt cgtggtcggc cagcacctgg ctcagcgcgt
     1081 cgcggtcgcc ggagacgatg ttgaccaccc cggcgggcag atcggacgtt tccagcacct
     1141 ggtacaggtc ggtcgcgctc aggggcgcgg cttgcgaggg tatggcgacg acggtattgc
     1201 ccatcgcaat ggcaggcgcg accagcgaga tgaagccgag cagtggatgg tcgtcagggc
     1261 aggccatgcc gatgacgccg atggcctccg gcagcgccag caccgcgccg cgcatgggcg
     1321 ggcgatgcac gacgccctcg tacttgtcgc accacgcagc atagctgaag agacgcgaga
     1381 tggcggcctg cacttctcgg gtggtgtcgg cgcgacccgt ctgctgcacg atgcgttgcg
     1441 cgaattcgcc ctcgcgaacc gagaggttct cggcgaggta atagagaatc tgcgcgcggt
     1501 tatgggtcgt gccgctcgcc caaccgcttg cggcgtgcgc caattcgacc gcg
//
LOCUS       DASZAK010000055         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_533764, whole genome shotgun sequence.
ACCESSION   DASZAK010000055 DASZAK010000000
VERSION     DASZAK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533764"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VGT44_00880"
     CDS             complement(<1..941)
                     /locus_tag="VGT44_00880"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2579377.1"
                     /translation="MSLAPPLSLVKQVVSQIFPSSCCRVERVEEGVSTYVHRIHQANN
                     VFYLRILPEEHASFVPEAQVHQQLLARSVKVPEIIYVEDNNELLQCSIMVTTEIKGTH
                     CGDCSSANSLQTILFEAGKDLAIINSIPVEGFGWIRRNGNKFTLLEAEHPTLRAFVYE
                     HLESDLAELAHIVLPEKDISAIHAILKRYDNLLDEDRSWLAHGDFDMTHIYQKQGRYS
                     GIIDFGEIRGTHALYDLSHFRVHDGEMLPILLFPYLLEGYYEMTALPHDYEQRIVLTS
                     LLITIRTLARAMKRHPQSFQEHHALKVLKRDVQALLA"
     gene            complement(1027..>2261)
                     /gene="pepF"
                     /locus_tag="VGT44_00885"
     CDS             complement(1027..>2261)
                     /gene="pepF"
                     /locus_tag="VGT44_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV2579378.1"
                     /translation="EEGNEVPLTNGNYLVYIRSKDRRVRKAAFDGMLGTFLKQRNTIA
                     ATLAAQVKSDIFHTRQRGFASCRERALSNYNIPESVYDNLVQTVGEYIPVMNRYMELR
                     KRMLNLDELHLYDLYVPIVEETTDEITYQQARDTVLNSLAVLGEGYQTVLGRAFSERW
                     IDVYETPGKRSGAYSGGAYTTHPFILLNFQNKRDSLFTLAHELGHSMHSHFTRTTQPY
                     QYGDYTIFVAEVASTLNEALLIDYLLRTTTDRQVRLALLNHALEDIRGTLYRQTMFAE
                     FELLTHSRAEAGEPLTADSLSELYHTLNAKYYGGVAVIDELIDIEWARIPHFYSSFYV
                     YQYATGISAANALAQQILREGQPAVQRYLRFLSSGSSDYSIELLKKAGVDMTSPEPVR
                     QTCLLFEDYLKQMEELLG"
BASE COUNT          522 a          565 c          622 g          552 t
ORIGIN      
        1 taggccagta gcgcctgtac gtcccttttc aataccttga gtgcatgatg ctcttgaaaa
       61 gattgtggat gtctcttcat tgcacgcgcc aacgttctga tggtgatgag caagctggta
      121 agaacaattc tttgctcata atcatgtggc agtgctgtca tctcgtaata gccttccagg
      181 agataaggaa ataacagaat aggcagcatt tctccatcgt gtaccctgaa atgactgagg
      241 tcatacaatg catgagtacc tctgatttcg ccaaagtcga tgattccgga ataccggcct
      301 tgtttctggt aaatatgtgt catatcgaaa tctccatgcg ccaaccatga ccggtcttca
      361 tccagcagat tatcatatct tttgaggatc gcatgaattg cagagatatc tttttcaggt
      421 aaaacgatat gcgctaattc tgcaaggtcg ctttccagat gttcatagac gaatgctctc
      481 agagttggat gctcggcctc aagtaaagta aatttattac cgtttcttcg tatccagcca
      541 aaaccttcta ctggaatgct gttgatgatc gcaaggtctt ttcctgcttc aaaaaggata
      601 gtttgcaggc tatttgcaga agagcaatcg ccacagtgag tccctttgat ttcagtagtt
      661 accatgatgg aacattgaag tagctcatta ttgtcttcaa catagatgat ttctggtact
      721 ttcacgcttc tggcaagtaa ttgctggtgt acttgcgctt ctggtacaaa gctggcatgt
      781 tcttccggca atatccgcag gtagaacaca ttgtttgcct gatggatgcg atggacatag
      841 gtggaaacac cttcttcaac acgttccacc ctacaacaag aggaagggaa aatttgtgaa
      901 acgacctgtt ttacgaggga taatggagga gcgagactca tcactccttc gttccgatga
      961 tagcatctaa ccagaagggc gcgatgaatc gcgcccctac acacattatc gattgccgaa
     1021 agaccatcat cccaacaatt cttccatctg cttgaggtaa tcctcgaaca gcaggcaggt
     1081 ttgccggacg ggttcgggcg aggtcatatc gacgcccgct tttttcagca gttcgatgct
     1141 gtagtccgaa gagccggacg aaaggaacct gaggtagcgc tgcacggcgg gctggccttc
     1201 gcgcaggatc tgctgcgcca gggcgttggc ggcggagatg ccggtcgcgt actggtagac
     1261 gtagaagctg ctgtagaaat gggggatgcg cgcccattcg atgtcgatca gctcatcgat
     1321 gaccgccacg ccgccgtagt atttggcgtt gagcgtatgg tacagctcgc tcagcgaatc
     1381 ggcagtaagg ggctcgcccg cttcggcgcg gctatgcgtc agcagctcaa actcagcgaa
     1441 catggtctgg cgataaagcg tgccgcgaat atcctccagg gcgtgattga gcagagctaa
     1501 gcgcacctgg cgatcggtgg tggtgcgcag caggtaatcg atcagcagcg cctcgttgag
     1561 cgtggacgcg acctccgcga cgaagatggt atagtcgcca tactggtaag gctgcgtcgt
     1621 gcgcgtgaag tgcgaatgca tggaatgacc aagctcgtgc gccagggtaa agaggctatc
     1681 gcgcttattc tggaaattga gcaggatgaa ggggtgcgtc gtgtacgcac cgccgctata
     1741 ggcgccgctg cgtttgcccg gcgtctcgta gacatcgatc cagcgctcgc tgaaagctct
     1801 gccgagcact gtctgatagc cctcgcccag gacagccagg ctgttcagca cggtatcgcg
     1861 cgcctgctgg taggtaatct cgtcggtcgt ctcctcgaca atcggcacgt acagatcgta
     1921 caaatgcaac tcgtccaggt tcaacatgcg tttgcgcagt tccatgtagc ggttcatcac
     1981 ggggatatac tcgccgacgg tctgcacgag gttatcatag acggattcag ggatgttata
     2041 gttcgacaga gcgcgctcgc gacaggaggc aaagccgcgc tgccgggtat ggaagatgtc
     2101 cgacttgacc tgcgccgcga gcgtggcggc gatagtgttg cgctgcttga ggaaggtacc
     2161 aagcatccca tcgaaggcgg ctttccgcac gcgccggtct ttgctgcgaa tgtaaacgag
     2221 gtagttgcca ttggtcaacg gcacctcgtt gccctcttca t
//
LOCUS       DASZAK010000056         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_569276, whole genome shotgun sequence.
ACCESSION   DASZAK010000056 DASZAK010000000
VERSION     DASZAK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569276"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1281
                     /locus_tag="VGT44_00890"
     CDS             <1..1281
                     /locus_tag="VGT44_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579379.1"
                     /translation="GQPYSGDLSGSAATAIAALALFAVLELERRRAPASNSVALFFQR
                     FHLYGVQFILLNILVFTWINAFSVLVNMLAYSGQASSAGINPCVGFVACANGPNLLSV
                     LAAALWMVVFWLGYGWLARSDAPSLFRRIVLAISFAYGIALLLSGIYLALALFFRFVF
                     GNAPTAVDALNGYNFTPYITLGLLVSAAYLAWLRPSARPQQAREPVLPLYCQAIIGAL
                     MAGAFFGGIGYVVVNLLELPAMPSDWAEALALIITGAAFIVFDLRLRQSNRTNVEGAL
                     EARRGFVFALLGAGVIAAAIGGATALYTWITAQIGSPITEWQHTAHIGLAAFIVGVVV
                     LALYLWQATREQLISNLGRRQTHTATASMPPSPAMPDHVATPPQPAGAEPQQPAPVAE
                     QATIEAVLDDLLAGKITRDVAVQRIRDLTLGVLP"
     gene            complement(1278..2012)
                     /locus_tag="VGT44_00895"
     CDS             complement(1278..2012)
                     /locus_tag="VGT44_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV2579380.1"
                     /translation="MISYWTILLRLVAALLLGSIVGLERERKERGAGMRTLALVTLGS
                     TLFTLVSAYGFLDLIGQLHLTLDPTRIASYIVAGVGFLGAGTIFMSRDREKVKGLTTA
                     ATIWVMAAVGIACGAGLLLEAISTTLLTLIVLVVLSYVEKRLAPTGSANAQHIRIEAS
                     MVNGELLSKVYETGARYGLVIEKLRIHSVEGADVIELSCRAVEASSIANALGALRALP
                     GVHLIQIDEMDAGEISNFLNKGKREV"
     gene            2363..3682
                     /locus_tag="VGT44_00900"
     CDS             2363..3682
                     /locus_tag="VGT44_00900"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2579381.1"
                     /translation="MTLEGKQVDRYHILHLLGSGGMGDVYLAEDPRIGQQVAIKVIRT
                     EVAPYPDENNAKDAARLFHREAKAIVRLDHPHILPLFDYGEERIGNMSIIYLVMPYRP
                     EGSLSDWLRKRETKQLLSPRDIAHFVAQAASALQHAHDHNVIHQDVKPSNFLVRISID
                     TSPLPDLLLSDFGIARLSSATSSVSQSVRGTPSYMAPEQWEGEPVPSTDQYALAVMAY
                     ELLTGKLPFQGGPGKMMYQHIHVQPQPTSALNPGLTGEIDKVVARALAKRPQERFDSV
                     SEFGIAFQQAVGSVSKVQSGSLLNQIEKPGVALTPIASDDAEGNTPTLLNSAMPDVGS
                     DSETFVKIEHSSTSTLKPPRKRKLTVVALIMLVVALIGGGVAYAVSSVTAASSGGHLG
                     LTTLTPNRSPVVTNATSASISIVPATKTLQNPFSIEAVTGGYSPTNR"
BASE COUNT          818 a         1080 c          972 g          848 t
ORIGIN      
        1 ggacagccct atagcggtga cctgagtggc tcagctgcga cagccatagc ggctctggcg
       61 ttattcgccg tactcgagct ggagcggcgg cgcgctcctg ccagcaacag cgtcgccctg
      121 ttcttccagc gttttcacct gtatggcgta caattcatcc tgctcaatat cctggtgttc
      181 acctggatca atgccttctc agtcctggta aatatgctgg cctactcggg gcaggcttcc
      241 agcgctggaa tcaacccttg cgtcggcttt gtagcctgcg cgaatgggcc gaacctgctc
      301 agcgtcctgg cggcggcgct gtggatggtc gttttctggc tgggctatgg ctggctggcg
      361 cgtagcgatg ctccctcgct attccggcgt atcgtgctgg caatcagctt cgcctatggt
      421 atcgccttgc tcctgagtgg catctacctt gccctggcgc tcttttttcg cttcgtattc
      481 ggcaatgcgc ccacggcagt tgatgcctta aacggctaca atttcactcc ttacatcacc
      541 ctgggtctgc tggtctccgc cgcctatctc gcctggctgc gcccatccgc gcgaccgcag
      601 caggcgcgcg aaccggtatt gccgctctat tgccaggcga ttatcggggc tttaatggcc
      661 ggggcatttt tcgggggcat tggttacgtc gtggtgaatc tgctggaact gcccgcgatg
      721 ccgtccgatt gggccgaagc gctggcgctc attatcacag gcgcggcctt tatcgtgttt
      781 gacctgcgac tgcgtcagag caatcgcacg aacgtcgagg gcgcattaga agccaggcgc
      841 gggttcgtct tcgccttgct gggcgccggt gtcatcgcgg cagccatcgg cggcgcaacc
      901 gcgctctata cctggatcac ggcgcagatc ggttcgccta tcaccgaatg gcagcacacc
      961 gcgcatatcg gcctggccgc ctttatcgtc ggtgtggttg tactggcgct ctacctctgg
     1021 caggccacgc gtgaacaact catcagcaac ctgggccgcc gccagaccca cacggctacc
     1081 gcgagtatgc cgccatctcc tgccatgccc gaccacgttg caactccccc tcagcccgcc
     1141 ggtgcagaac cacagcagcc agcgccggtc gcggagcagg ctaccatcga agccgtcctg
     1201 gatgatctgc tggcagggaa gatcacacgt gatgtggccg ttcagcgtat ccgcgatttg
     1261 acgctcggtg tactccctta aacttccctt ttacctttgt tgaggaaatt gctgatctcc
     1321 ccggcgtcca tctcatcgat ctggatcaga tggacgccgg ggagggcgcg caaggcccca
     1381 agcgcgtttg cgatggacga tgcctcaacc gcacggcagg ataactcgat cacatccgcg
     1441 ccctctacgg aatgaatgcg cagcttttcg atgacgagtc cgtagcgcgc tccggtctca
     1501 tagaccttcg acaacaactc accattgacc atgctcgcct cgatacggat atgctgcgca
     1561 ttcgccgaac cagtaggcgc aagacgtttc tcaacatagg acaggacaac cagtacaatc
     1621 aacgtcaaca gcgtcgtcga gatcgcttcc agcagcagac ccgcgccgca ggcaataccg
     1681 accgccgcca tcacccagat tgttgccgcc gtcgtcagtc ccttgacctt ttcccggtcg
     1741 cggctcatga agatcgtacc cgcgccgaga aatcccacac cggccacaat gtaggatgca
     1801 atgcgcgttg gatcgagcgt taaatgcaat tgaccgatta agtcgagaaa gccgtacgcc
     1861 gagacaagtg tgaacagcgt cgagcctagc gtcaccagcg ccagtgtgcg cattccagct
     1921 ccgcgctcct tccgctcgcg ttccagcccc acaatcgatc ccaggagcag agccgctacc
     1981 aatcgcagta agattgtcca gtacgaaatc atgagcttcc ctcatccttc cgacacgtca
     2041 gtatgcaggt cggttcatcc gattcatgtg acaattatac agcttgccgg aaggataggt
     2101 atagcctatt gtggacatga tctgtagggg cggcgtggag acgcctgcgt tgcccggacg
     2161 gaggtcgtcg cataagcccg ttgctaggaa aaaatgctac tataatacct ggttacttta
     2221 cgaaatggta ttgtgggcgc gaggcgcatc agtgctatca ggagaactat gcattcactt
     2281 gagaatatgg taatatgtat gatacattca acatcttcag tgtaagcaat ttctgtatgt
     2341 ttctatgagg gggagatgaa atatgacact cgaaggcaag caagttgacc gctatcatat
     2401 tctacaccta ttgggaagcg gtggtatggg cgatgtctat ctcgcagaag atccgcgcat
     2461 aggtcagcaa gtcgccataa aagtaatccg aacagaagta gcgccatatc ctgacgagaa
     2521 taatgcgaaa gatgcagcgc gactcttcca ccgcgaagca aaagctatcg tcaggctcga
     2581 tcatccacat atattaccac tcttcgacta tggcgaagag cgtatcggca acatgtccat
     2641 tatctacctc gtcatgccat accggccaga ggggtcgctc tcggattggc tgcgtaagcg
     2701 cgagacgaaa caactgctct cgccacgaga tattgctcac tttgtcgccc aggcagccag
     2761 cgcgcttcaa catgcccacg atcacaatgt cattcaccag gatgtcaaac cctctaactt
     2821 tcttgtacgt attagcatag atacgtctcc gctcccggat ctgttgctct cggattttgg
     2881 aatcgcacgt ctatctagcg ccacctccag tgtcagccaa tcagtgcgtg gtactccttc
     2941 ttatatggcc cccgaacagt gggaaggcga gccggtaccc tccaccgatc aatatgcact
     3001 ggcggtaatg gcgtacgaat tgctaacagg caaactccca ttccagggcg gccccggcaa
     3061 gatgatgtac cagcatattc atgtgcagcc tcagccaacc agtgctttga accccggtct
     3121 cactggagag attgataagg tagtcgctcg agcattagcg aagagaccac aggagcgttt
     3181 tgactcggtg agcgagtttg gaatcgcttt tcagcaagct gtcggcagtg tatcaaaggt
     3241 tcaaagtggt tccctcctca atcagatcga aaagcctgga gtggcattaa ctcccattgc
     3301 tagtgatgat gcagagggaa acacgccgac attgctcaac tcagctatgc cagatgttgg
     3361 cagtgactca gagactttcg tcaaaataga gcattcttct acctctacgc taaagccgcc
     3421 caggaaaaga aagttgacgg ttgttgcttt aattatgttg gtagttgcgc ttattggtgg
     3481 gggtgttgcc tacgccgttt cctccgttac agctgcatct tctggaggac atctcggctt
     3541 aactactctg actccaaata gatcgccagt tgttaccaac gcaacctcag ccagcatatc
     3601 cattgtacct gctacaaaaa cattgcaaaa tccattttca attgaagcag tgacgggagg
     3661 gtacagcccc actaaccggt gaaataaggg agaaggattg aaaaaaacga cgctcctc
//
LOCUS       DASZAK010000057         1525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_498205, whole genome shotgun sequence.
ACCESSION   DASZAK010000057 DASZAK010000000
VERSION     DASZAK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498205"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1347)
                     /locus_tag="VGT44_00905"
     CDS             complement(<1..1347)
                     /locus_tag="VGT44_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEV2579382.1"
                     /translation="MIEEGITVGGHSLREHLEATNHAEAFDYLCTLVETTTPFTIETI
                     LTLHSLVLKTLNPTAGQFRTTPDDICGSELALPHSTQVPTMVAQWVAWLRGDGLFYEP
                     VVRAALAYHRFVAVHPFEDGNGRTGRLLLNLLLMRDGYAPAFLLRSWAERHRSALRAA
                     DQGKYNPIVNLVGQAVEAGLDFYLEACNAPPDERYQPLPELARSTGRDPNYLGLLVRQ
                     GKLEAIKRQGRWYSTLAALQLYDQQAQEGIRQRGRPRLYEAAALLSAERARRPQPPDS
                     LRPARAPATTPPSSAAGTPSLHLLTTKISVPPARLHIVSRPRLTQRMHAAIRGSLTLL
                     AAPAGWGKTTLLHAWSAEADRDAWPLAWVSLDASDNDPIRFWTYVLTALNTLHPGVGE
                     TPLAQLYASPPPPIEAVLTILLNALIELPTDTVLVLDDFHHIEAQPIHDALTYLAEH"
BASE COUNT          331 a          398 c          485 g          311 t
ORIGIN      
        1 atgctccgcg agataggtga gcgcatcgtg gatgggttga gcctcgatat ggtgaaagtc
       61 atccagcacc agcacagtgt ctgtggggag ctcgatcagg gcattcagca ggatcgtgag
      121 cacagcctcg atcggcggtg gcggcgaggc gtacaactgc gccaggggag tctcgcccac
      181 ccccgggtgc agcgtattga gggcggtaag gacataggtc cagaagcgga tgggatcgtt
      241 atcactcgca tcgagtgaga cccaggccag gggccaggca tcgcggtcgg cctcagcaga
      301 ccaggcatgg aggagggtcg tcttgcccca gccagcaggg gctgcgagaa gggtaagtga
      361 gccccggatg gcagcatgca tccgttgcgt gagccgtggg cgcgatacta tatgcaagcg
      421 ggccggcggg accgagattt tcgtcgtcag caggtggagg gacggcgtac cggcggcaga
      481 ggagggtgga gtggtagcag gagcgcgagc aggacggagc gagtcgggtg gctgcggtct
      541 ccttgctcgc tctgccgaca gcaaagcagc cgcttcataa agccggggtc ggccgcgttg
      601 acggatgcct tcctgggctt gttgatcata gagctgcaac gcagctaacg tgctgtacca
      661 gcgaccctgg cgcttgatcg cctccagttt gccctggcga accaagagtc ccaggtaatt
      721 gggatcacgt cctgttgacc gcgcaagctc tggcaatggt tgatagcgtt catcgggcgg
      781 ggcattgcaa gcttccaggt aaaaatctaa accagcttca actgcttgac cgaccaggtt
      841 gacgatcggg ttgtacttgc cctggtcagc agctctgagt gcgctacgat ggcgttcagc
      901 ccagcttctg agcaaaaacg caggcgcata gccatcacgc atcaagagaa gattcagcag
      961 taatcgccct gttcgaccat tgccatcctc aaaaggatga acggcgacaa acctatgata
     1021 ggctaatgca gcacgtacca caggttcata gaacagccca tcgcctctca accacgcgac
     1081 ccattgggct accattgtag gtacttgtgt agagtggggc agcgccagct cacttccaca
     1141 gatgtcgtca ggggtggtgc ggaactgccc agcagttgga ttcagcgtct taagcacgag
     1201 ggaatgcaac gtgagaatcg tctcaatggt gaaaggagtg gttgtctcaa ccagggtaca
     1261 taagtaatca aatgcctcgg catggttggt ggcttccaag tgttcacgca gcgagtggcc
     1321 accaacggtg atcccttcct cgatcacgat ccgcgtttcg cgcagattga gtgtattacc
     1381 tgcaagagca ttactgtggt acgttaatcg gattcgtaca tccttatgca ggcgctgcat
     1441 gattccgaga cggaacgggc ggtagctatc aagtgtggtt ttcttccgag ataaccgttc
     1501 tgccaggcgt ttgtcgagtc gtggt
//
LOCUS       DASZAK010000058         1918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_1379, whole genome shotgun sequence.
ACCESSION   DASZAK010000058 DASZAK010000000
VERSION     DASZAK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..657)
                     /locus_tag="VGT44_00910"
     CDS             complement(<1..657)
                     /locus_tag="VGT44_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV2579383.1"
                     /translation="MQPLTEEQVRFEIDMCIGCDHCMRACPIPMSSMVNIADLNRATI
                     SDDIAPHVARFTDECVMCGSCVPVCPVDNHRDLLMLSLKQRLGVSWDGTVDMSRVLNY
                     LPPGWDLPLLLSRLREQPMFSDPQLVLDNYLLHFVATSKLLMLMPGAIVIREGEFGRA
                     MYFILEGRFELSSAGPDGSDMPVAVLRRGEYMGEHGMLTGQQRNATARARTAAVVLEV
                     P"
     gene            complement(733..>1918)
                     /locus_tag="VGT44_00915"
     CDS             complement(733..>1918)
                     /locus_tag="VGT44_00915"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2579384.1"
                     /translation="EWLDKQGVPPPILSVSREGAVAASRATIHDQDAQSSMVGVGDPV
                     WGTGNAPALEEAFLVPVGQADGTPRLTEMQLSQTRVMTIGRQAGVYLLIDEGSVSRRH
                     AEVAPRFIEGARFIEGNFFGQYVLRDLGSSYGTFVNDQRLEPGSIAILKQGDRIRFGN
                     KVTYTFQVHFVAQQARRLTTLHELATGFYDPTAAGQAAPPLGQPVLNADGSLQLPGAT
                     SAVPAAVVATLKESPALIMIPTINRGTTGKPEVFHLKQGKRLTLGRDKNNDIILADVA
                     ASRLHAEVLHGLDGVYIRDLGSSNGVMVNRTKIDNPYRLAHGDRIAIGSIMLYFIHVA
                     PRFIVGALDEVPGRGQAPPLLEEAAQSYICRNCGTANTRIARFCASCGAPLENRQVAG
                     KR"
BASE COUNT          425 a          535 c          561 g          397 t
ORIGIN      
        1 tggtacctct agcacgacgg ctgccgtccg ggcacgtgcg gttgcattgc gctgctgacc
       61 ggtgagcatg ccatgctcgc ccatatactc accgcgccgc aggaccgcta caggcatgtc
      121 gctgccatct ggtcctgcgg aggaaagctc gaagcgtcct tccaggatga agtacatagc
      181 gcggccaaac tcgccttcgc gaatcacaat agctccgggc atgagcataa gcagcttgga
      241 ggtagcgacg aagtgcagca aatagttgtc gagtacgagt tgtgggtcac tgaacatggg
      301 ctgttcgcgc agacgcgaga gcaacaatgg caggtcccag ccgggtggga gatagttcag
      361 tacacggctc atgtctactg tcccatccca ggagacgccc aggcgctgct tgagcgagag
      421 catcaatagg tcacggtggt tatcgacggg acatacgggc acacaagagc cgcacatcac
      481 acattcatcg gtgaaccgcg ccacatgggg agcaatgtca tcggagatgg tggcgcggtt
      541 gagatcggcg atgttgacca tagacgacat agggatcggg caagcacgca tgcagtggtc
      601 gcaaccgatg cacatgtcga tctcgaagcg cacctgctct tcagtgaggg gttgcaagtt
      661 tgtgcggtgc tcaatgccta cgacaagggg ctgttttttt gagcttgatg attgtatctg
      721 catactatct ctctaccttt tgcccgcaac ctggcgattt tccagaggtg cgccacagct
      781 tgcacagaaa cgcgctatac gagtattcgc cgtgccacaa ttacggcaga tgtaggattg
      841 cgctgcctct tcgagtaggg gcggagcttg cccccgtcct gggacctcgt caagggctcc
      901 cacgataaat cggggagcta catgaataaa atagagcatg atactgccta tagcgatacg
      961 atctccatgc gcgagacgat agggattatc gatcttagta cggttaacca tcacaccgtt
     1021 gctgctgcca aggtcgcgaa tgtagacgcc atcgagaccg tgtagtacct cggcatgcaa
     1081 acgcgaggca gctacgtcgg ccagaataat atcattgttc ttgtcgcgtc ccagcgtcaa
     1141 gcgcttgccc tgtttcaagt ggaagacctc cggcttaccc gtagtccccc gatttatcgt
     1201 ggggatcatg atgagcgcgg gcgattcttt gagtgtagca acaacggcgg cagggacagc
     1261 gctggttgcc ccaggaagtt gcaaagagcc gtcggcattg agaacgggct ggcccaatgg
     1321 tggtgcggcc tgaccggcgg cggtaggatc gtagaatccg gttgccaact catgcagcgt
     1381 cgtcaagcgt ctggcttgct gggcgacgaa atggacctgg aacgtatacg tcactttatt
     1441 accgaaacgg atgcggtcac cctgcttgag aatagcgata ctgcctggtt ccaggcgttg
     1501 gtcattgacg aaggtcccat aggaactgcc caggtcacgc agtacatatt gcccaaagaa
     1561 attcccctcg ataaatcggg ctccctcgat aaatcgggga gctacttcgg cgtggcggcg
     1621 tgagacagaa ccctcatcga tcagcaggta gacgccagcc tggcgaccaa tcgtcataac
     1681 cctggtctgg ctcaattgca tctcggtcaa gcgaggtgta ccgtcggcct ggccgacagg
     1741 cacaagaaaa gcttcctcca gggcgggggc attacctgtc ccccagacag ggtcccccac
     1801 ccctaccata ctcgattggg catcttggtc gtgtatggtc gccctggaag cggcaacagc
     1861 accctctcgg ctcacactca ggataggcgg cggcaccccc tgtttgtcga gccactcg
//
LOCUS       DASZAK010000059         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_36645, whole genome shotgun sequence.
ACCESSION   DASZAK010000059 DASZAK010000000
VERSION     DASZAK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36645"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..408)
                     /locus_tag="VGT44_00920"
     CDS             complement(97..408)
                     /locus_tag="VGT44_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV2579385.1"
                     /translation="MDTLNLGRFSDPPILILASLAGSPKHGYAIMEDIETFARVHLGP
                     GTLYGAIARLETQGLIEPLPTEERRRPYRLTAAGIAFLQEQLTGLETFASMGLRRLAS
                     I"
     gene            778..1902
                     /locus_tag="VGT44_00925"
     CDS             778..1902
                     /locus_tag="VGT44_00925"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEV2579386.1"
                     /translation="MSIALNKVQTRFLRQYAQRLTPRQPGESAAQVVKDVCGIQAQDA
                     RAAALSIRSRGAGLVAADIEQARVQDRSIARAWGPRGTLHLLASEDLGWLLMLFGPVF
                     IAGDRARRLELGLDDETSARGVREIRDILADQGPLTRDELVEQLAARGLRLEGQARPH
                     LIFYAALQGILCLGPDRGSKPTYVLLDDWLGHIDSTTLSREAACAELARRYISAYGPA
                     SRDDMAAWSGLPISEIRAAWKHIANSLLEVEIAGQPAWMLKSHLARLDEFSVDHPIVH
                     LLAAFDTYLLGYRSRDMLVEQQYAKRINAGGGMIRPALLVDGRAAGTWQYSPKRKRLD
                     VLVVPFEPLASAVLSGMEEEVGDVARFLEIEATALTILSE"
     gene            complement(1913..>2317)
                     /locus_tag="VGT44_00930"
     CDS             complement(1913..>2317)
                     /locus_tag="VGT44_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2579387.1"
                     /translation="QNAHLFPSLTAQENVEVALRLQGTPPDERGRRALETLELVGLSA
                     RSHHRALELSGGEQQRAALARALVHNPRFVVADEPTGNLDSQTGRAIVTLLHDIVRKT
                     GVGLLVATHDALVYGQADRVAQIQDGMMVTSG"
BASE COUNT          464 a          681 c          694 g          478 t
ORIGIN      
        1 aagagatagg gcgttgctcc agcatggcca agaactcttc ctcatagcgt ttgcgccagg
       61 tatacggata gaagcgcagc aaataccttt ctatgctcat atgcttgcta acctccgtaa
      121 acccatactg gcaaatgttt ccagtccggt aagctgttct tgcagaaatg caatcccggc
      181 tgcggtcaaa cgatagggac gccgtcgctc ttccgttgga aggggttcga tcagcccctg
      241 cgtctccaga cgagcaatag cgccgtacag cgtgccgggt cccaggtgga cacgtgcgaa
      301 ggtttcaata tcctccatga tggcatagcc atgctttgga ctcccagcaa ggcttgctaa
      361 gatcagaatg ggtgggtcag aaaatcgccc cagattgagc gtatccatac acggtttctc
      421 cagatatttg atatacgttc agcgttatat cgttcaacgt agtacgttga acgtagtata
      481 tagtatgaat gcagggtctg tcaacgtcct ttctcgctgt agtatgctac acttaaggag
      541 gcaattgcac tcgatcatgc ccagttgtag ccagcgtcgt aggaacgcga ttatgaatca
      601 tcacaaccag ggtcggtcat cgattgtcac cctgagcgaa gcgaagggtc tcggtcgagg
      661 ggcacagaga tgcttcgctg cgctcagcat gacattcctg actgtggtta ggaatgttca
      721 taatcgcggc cctacgccgc tggccaggat atttgtcaat ctccataaat agcatttatg
      781 tcaatagctc tgaataaagt tcagacgcgc ttcctgcgtc aatacgcaca gcgcctcact
      841 ccgcgccagc caggcgaaag cgccgcccag gtcgtgaagg atgtgtgcgg catccaggcg
      901 caggacgccc gcgcggcagc cctatcgata cgctctcgcg gcgcggggct ggtggccgcc
      961 gatatagagc aggcgcgcgt gcaggatcgt tcgattgcgc gcgcgtgggg gccgcgcgga
     1021 acgctgcatc tgctcgcaag cgaagacctg ggctggctgc tcatgctctt tggcccggtc
     1081 ttcatcgccg gagaccgcgc gcggcgtctg gagcttgggc tggacgacga aaccagcgcc
     1141 aggggtgtgc gcgaaattcg cgatattctt gccgaccagg ggccgctgac gcgcgacgaa
     1201 ctggtcgagc agcttgccgc gcgcggcctg cgcctcgaag gtcaggccag gcctcacctc
     1261 atcttctacg ctgccttgca gggcatcctc tgcctggggc cagatcgcgg ctcgaagccg
     1321 acctatgtcc tgctcgacga ttggctagga catatcgatt ccacgactct atcgcgcgaa
     1381 gctgcctgcg cggagcttgc gcggcgttac atctcggcct atggcccggc cagtcgagac
     1441 gatatggcgg cctggtcagg tctgcctatc agtgagatac gcgccgcgtg gaaacacatc
     1501 gcgaactccc tgctggaagt ggagatagcc ggtcaacccg cctggatgct caaatcccac
     1561 ctcgcgcggc tggatgagtt ctctgtcgat caccccatcg tgcatctact ggcagctttc
     1621 gacacgtacc tcctcggcta tcgcagccgc gatatgctcg ttgaacagca gtatgcaaaa
     1681 cgtatcaacg cgggcggcgg catgatacgt ccagcgctgc tcgtcgatgg gcgggcggca
     1741 ggaacgtggc agtatagccc taaaaggaag aggctggatg tcctggtagt gccgttcgag
     1801 ccgcttgcat ccgctgtgtt gtcagggatg gaggaagaag taggcgacgt agcgcgcttt
     1861 ttggaaatag aggctaccgc actgactatc ctcagtgaat aagagagatt actcacccgc
     1921 tcgtcaccat catcccatcc tgaatctgcg ccacccggtc ggcctggcca tacaccagcg
     1981 cgtcatgcgt ggcgaccagc agaccaacgc ccgttttgcg cacaatgtca tgcaatagag
     2041 tgacgatggc ccggcctgtc tgcgagtcca ggttgcccgt cggctcgtcg gcgacgacga
     2101 agcgcgggtt gtggacgagc gcgcgggcca gggccgcgcg ctgctgctcc ccgccggaga
     2161 gttccagggc gcggtgatgc gaccgtgcgc tcagccccac cagctccagc gtttcgaggg
     2221 cgcgccgacc gcgttcatcg ggaggcgttc cctgcaggcg cagcgcgacc tctacgtttt
     2281 cctgcgcggt aagcgatggg aaaaggtgcg cattctg
//
LOCUS       DASZAK010000060         3394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_72469, whole genome shotgun sequence.
ACCESSION   DASZAK010000060 DASZAK010000000
VERSION     DASZAK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72469"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..450
                     /locus_tag="VGT44_00935"
     CDS             31..450
                     /locus_tag="VGT44_00935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV2579388.1"
                     /translation="MSTVIERANFPDLGREQCIALTTFRKSGQAVTTPVWFAQRLGTI
                     YVETHGDAGKLKRLRHTNRVTLAPCTYSGKVTGAVSTGKARILTEPEKCTAASTALAK
                     KYGFMRPLFHFVRNAQRLLQRKAKVEVVYIAIEPISG"
     gene            complement(578..988)
                     /locus_tag="VGT44_00940"
     CDS             complement(578..988)
                     /locus_tag="VGT44_00940"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEV2579389.1"
                     /translation="MMKTPYGFGTRLPLPYYDAVGRVKDALKTEGFGVLTEIDVRKTM
                     REKLGVEMEPYVILGACNPPLAHRALEMEPDIGLLLPCNVVVRADGLGSRVDIADPRA
                     MLGIVGNEELNAVAEEARQRLQRVVTALDSVDQH"
     gene            1374..1790
                     /locus_tag="VGT44_00945"
     CDS             1374..1790
                     /locus_tag="VGT44_00945"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEV2579390.1"
                     /translation="MEDRMSIIVFSGTVDKLMAASILATGGAAMGMEVEVFLTTWGLE
                     AFRKDGYKTNMKVSKDFEEYGPVMMEQMMAKKVPSWMENLKSAMEVGDVKIYACSMTM
                     ELFDMKLSDLEPIVTDVTGVATFVERAKEGRFTLFI"
     gene            1824..2072
                     /locus_tag="VGT44_00950"
     CDS             1824..2072
                     /locus_tag="VGT44_00950"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEV2579391.1"
                     /translation="MPELKEITIDKEIDARGSFCPGPLMELIRGMKALPVGGVLAVLS
                     SDPGSAKDIPAWIQKARHEFIGTFPEQGYTRFVARKAH"
     gene            2088..3296
                     /locus_tag="VGT44_00955"
     CDS             2088..3296
                     /locus_tag="VGT44_00955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEV2579392.1"
                     /translation="MPQHILIVGGGVGGTIVANLLARHLEPVDAKVTLIESTGKHAYM
                     PGWLYMPFNHEGLESEQVIRDERGLLNRHVQHVRGTVTGIDTQNRELHVHYSPGLDEI
                     VGTGGAVEATYGYDYLVLATGARLAPEDLDGLSEANGKGGWHHFYSAEAALQLRHALR
                     RFEGGRIVITVGGIPYRCPPAPLEFTFLLDEWLAKHDLRSKTEIQYLFPLPRVFPIES
                     VAEAVTPLLEERHIGYTVFFNAETVDTEKKIISSLEGEEIPYDLLVLVPPHRGARVIE
                     ESGLGDEQGWIPTDRNTLEVKNHPHVYALGDATDLPVSKSGSAAHFEAKIVAARILAA
                     IRGEEPKPDEATYDGEVMCFLETGYNKATQLVFNYENPPRPPRPSLYYHMEKQLFNRA
                     YWHIVPQGLV"
BASE COUNT          747 a          950 c         1025 g          672 t
ORIGIN      
        1 ctcttgcgcc acgcgcacag gaggtgaacc atgtctacag ttatcgagag agcaaacttt
       61 cctgacctcg gcagagagca gtgtattgcc ctgacgacgt tccgcaagag cgggcaagcg
      121 gtgacgacac cggtgtggtt cgctcaacgt ctcggaacga tctatgtcga aacacacggg
      181 gacgcaggca agctcaaacg tctacgccat actaaccgcg tcacgctcgc tccctgtacc
      241 tacagtggca aagtcaccgg agcggtgagc acgggcaagg ctcgcattct gacggagcct
      301 gagaagtgta cagcagcttc gaccgcactg gcaaagaaat atggcttcat gcgccctctc
      361 tttcacttcg tcagaaatgc ccagcgcctg ctccaaagga aggcgaaagt tgaggtcgtc
      421 tacattgcga tcgaacccat ttcaggctga acctggtggg cagaactgcg atgaggatgg
      481 tgaataacgg aaggagagaa aagcatgtca ccgtttacaa gagagcaagt actcgaattg
      541 cttcaatcaa atacgagcct ggcaggtgtg ttcaattcta atgttggtct acgctgtcga
      601 gcgcagttac gacgcgctgc aagcgctgcc tggcctcctc tgcaacagca ttgagttcct
      661 cgttgccgac gatgccgagc atggcccgcg ggtcggcgat gtctacacga ctccccaggc
      721 catctgcccg cacaaccacg ttgcacggta gcagcaggcc gatatccggc tccatctcaa
      781 gcgcgcggtg cgccaggggc gggttgcatg cgcccaggat gacgtagggt tccatctcaa
      841 cgccgagctt ctcgcgcatg gtcttgcgca catcgatctc ggtgaggacg ccgaacccct
      901 ctgtcttgag cgcatccttc acgcgcccaa ccgcatcgta atacgggaga ggcaaccgtg
      961 tgccaaaacc gtagggtgtt ttcatcatgg cttgctgctc cacttcatcg ctctctggtt
     1021 ccgtgtcata acttcgctcc tatatattca taatatcgaa taatcgaatt atggcatgag
     1081 catcgacggc tgtcaactat tcactttcct gcctgtcatc ctgagcgcgg tgagatcctt
     1141 cgctgcgctc aggatgacag gcgctgactg cgctcaggat gacagccatt ctctccaaac
     1201 atgtgggaaa ggggtagtgt gattgacctg tgatagtgca agcccatgct gtgattgcac
     1261 agtgaggtcc ggcctgtata gtgaatagtg cataacgaat atgcgaatga ttgaataatg
     1321 atcctgaatg cctggaaaca ggcggggtca agtgcgcaca aaggagcaaa acgatggaag
     1381 atcgcatgtc gatcatcgtc ttttcaggaa ccgttgacaa actgatggcg gcatctattc
     1441 tcgccaccgg cggggccgcc atgggcatgg aagtggaagt cttcctgacc acctggggcc
     1501 tggaggcgtt ccgaaaggat ggatataaga ccaatatgaa ggtcagcaag gatttcgaag
     1561 agtatggccc ggtcatgatg gagcagatga tggcgaagaa ggtcccctcc tggatggaga
     1621 acctcaagag cgctatggag gtgggggatg tcaagatcta tgcgtgcagc atgaccatgg
     1681 aactgttcga tatgaagctc tcggacctgg agccgattgt gaccgatgtc accggcgtcg
     1741 ccaccttcgt cgagcgcgcc aaggaaggcc gtttcacctt gttcatttaa caagggaggg
     1801 ccttgtgaaa ggagttggtt ctcatgccag aactgaaaga gatcacgatt gataaagaga
     1861 ttgatgcgcg cggcagcttt tgccctgggc cgctgatgga gcttattcgc gggatgaagg
     1921 cgctgccggt gggcggcgta ctggccgtgc tgtccagcga tccgggttca gccaaagata
     1981 tcccggcctg gatacagaaa gccaggcacg aatttatcgg aacgttcccg gagcaggggt
     2041 acacccgctt tgtcgcgcgt aaagcccatt gagaaggagg cagcgttatg ccccaacata
     2101 tcctgattgt gggcggcgga gtaggcggca caattgtcgc caatctcctg gcgcgccacc
     2161 tggagccagt tgacgctaaa gtcacgctca tcgagagcac cggtaaacat gcctatatgc
     2221 ccggctggct gtatatgccg tttaaccacg agggcctgga gagcgagcag gtcatccgcg
     2281 acgagcgcgg tctgctcaac cggcatgtgc agcacgttcg gggtacagtg accggcatcg
     2341 atacgcagaa ccgcgagctg catgtgcatt acagcccggg tctggatgaa atcgtcggta
     2401 ccggcggagc ggtcgaggcg acctacggct acgattacct ggtgctggca acgggtgcgc
     2461 ggctggctcc ggaggacctg gacgggttga gcgaggcgaa cggcaaaggt ggctggcacc
     2521 acttctacag cgccgaggct gcgctgcaac tgcgccacgc gctgcggcgc ttcgagggcg
     2581 ggcgcatcgt catcaccgtg ggcggcattc cttatcgctg cccgcccgcg ccgctggagt
     2641 tcaccttcct gctcgatgag tggttggcga aacacgacct gcgcagcaag accgagatcc
     2701 agtacctctt cccgctgccg cgcgtcttcc cgattgaaag cgtcgcggaa gccgtcacgc
     2761 cgctcctgga ggagcgtcac atcggctaca cggtcttctt caacgccgag acggtcgata
     2821 cggagaagaa gatcatctcc tcgctggaag gggaagagat tccctatgac ctgctcgtgc
     2881 tggtgcctcc ccatcgcggg gccagggtga tcgaggaatc cgggctggga gatgaacagg
     2941 gctggatacc aaccgaccgc aacaccctgg aagtgaagaa tcatccacat gtctatgcgc
     3001 tgggtgacgc caccgacctg ccggtctcca agagcggatc agccgcgcac ttcgaggcta
     3061 agatcgtcgc cgctcgcatc ctcgcagcca ttcggggtga ggagccgaag ccggacgagg
     3121 cgacctacga tggcgaagtg atgtgcttcc tggaaaccgg ttacaataag gcgacgcagc
     3181 tggtcttcaa ttatgagaat ccgccgcgcc cgccgaggcc cagtctgtac tatcacatgg
     3241 agaagcagct tttcaaccgc gcctactggc acatcgtgcc gcaggggctg gtctaggcgc
     3301 gaacttggat tacaggcggg cggcataacg ccgcctgctt ctcttcacga ggctgcgtgc
     3361 gcagcgagcg gccgcacgac gaggacctgc agcg
//
LOCUS       DASZAK010000061         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_321063, whole genome shotgun sequence.
ACCESSION   DASZAK010000061 DASZAK010000000
VERSION     DASZAK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321063"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..614)
                     /locus_tag="VGT44_00960"
     CDS             complement(279..614)
                     /locus_tag="VGT44_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579393.1"
                     /translation="MSQQDLDYNEMNRDRPGFSSRTYEGEGTHRYNTYGDGDKLSAPV
                     LRASLTAGQRLTLAIVSLAMIMLMTFGLIGISVATQAPAWVVLPILFILVLFTAAAVI
                     INIVFNRKP"
     gene            836..1762
                     /locus_tag="VGT44_00965"
     CDS             836..1762
                     /locus_tag="VGT44_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HEV2579394.1"
                     /translation="MAEPSEERISAVRQFNRFFTRQIGVLREGLLHSPYSLTDARILF
                     ELGQGSRVTASRLGRELGLDAGYLSRILARLEQQGLLEKVRSENDGRQLLLSLTAAGR
                     EAFALLDRRSREEVAEMLDDLGEEDQQRLLKAMQTIESLLTNGFKFSQPFVLRPHEPG
                     DMGWVTHRHGVLYAQEYGWDEHFEALVAQIVADFINNYNPARERCWIAEMDGEIIGSV
                     FVVQSSETVAKLRLLLVEPKARGLGLGTRLVEECIRFARRHGYQKLMLWTNSILLEAR
                     HIYKKTGFQLIAEEAHHSFGHDLVGETWELVL"
     gene            1941..>2974
                     /locus_tag="VGT44_00970"
     CDS             1941..>2974
                     /locus_tag="VGT44_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579395.1"
                     /translation="MRSKTAELSRAMQPRWRRRLSTVLSLLLGVVVVLLLASLSAPVT
                     HVAFANPAGGNLPVIHWDASMIYAGQNNGYPWGPVGENTIVHGANFNPNTQLHLILAP
                     GDSNSNPAVCRQPTVTVLVATVTADTTGGFTQSFPWPSAAGQVNQAYSICSLRMSNNT
                     VASYLDDGPFTVLSSSPPLIALSASSVAAGNAITVTGRYWVPPQPVSVIIASCADCGG
                     AVLVANTGANSTGLNSGSFSVSIPIPATAKPGDYVVNALTTTGLDANYTSGVKRLAIT
                     AAPTTQPSPTPTPTLQPSPTTSPTVTATATTAASATPAASATSNGASGTVTTTSNGSS
                     GTTGTGTSSG"
BASE COUNT          681 a          832 c          798 g          663 t
ORIGIN      
        1 cattgagcta gcagcccggt atttgtgcgg gtataaagta acatataaat gaactcgcgt
       61 caagcatagc tttctacaat tcatagtcct ttgcccaccg tacatttttc tcctggttgt
      121 tctttgatgg tgtcaatcga agagttgcgc ctgttatgcc tacatcatcc acagggatga
      181 agtagacttt gttgagtgct tcaacatata tagcaaagaa ttcgcactgg ccgcgatagc
      241 ttctagctcc tttgtgtttg actgtgtagt tcttgctgct agggcttgcg gttaaacacg
      301 atattgataa taactgccgc ggcagtgaac agaacaagga tgaaaagtat tggcaaaacg
      361 acccaggcgg gagcttgcgt agctactgaa atgcctatca gaccgaaagt cataagcata
      421 atcatggcaa gtgaaacaat cgcgagggtc aagcgttgac ctgcggttaa tgaggctctc
      481 aatacaggtg ccgagagttt atctccatcc ccataggtgt tatagcgatg tgtgccttcg
      541 ccctcatatg tcctggagga aaatcctggt ctgtcgcggt tcatctcatt gtagtccaaa
      601 tcttgttgcg acatgatagt taccctctct ctttaccaga cacagaatag tacgtaccct
      661 gccgttttca atacgagaaa gacacgtaaa agttgtaagt agaaacttgc atatcgggtt
      721 agccaggtgg ccacaaacgg gtagtatctg caatatctcc ttgacaagag caagcatata
      781 atagataata acagatagat aattgctttt gtcaaacaaa tgaaaggagc acgtaatggc
      841 tgaaccttcc gaagagagga ttagcgctgt ccggcagttc aatcgtttct tcaccaggca
      901 gatcggcgtt ctgcgcgagg gcctcttgca cagcccctat tcgcttaccg atgctcgcat
      961 cctctttgag cttgggcagg ggagccgggt gacggcttcc aggctgggtc gcgagctggg
     1021 tcttgatgcc ggatacttga gccgcatcct ggcgcgtctc gaacaacaag ggctgcttga
     1081 aaaagttcgt tccgagaacg atggacgcca gctcctcctc agcctcaccg cggcaggcag
     1141 agaggcgttc gctctcctcg accggcgatc acgcgaagag gtagcagaga tgctggatga
     1201 tctcggcgaa gaggaccagc agcgtttgtt gaaggccatg cagaccatcg aaagtctgct
     1261 caccaatgga ttcaaatttt cacagccgtt tgtcctgcgc ccgcacgagc ctggcgatat
     1321 ggggtgggtc actcaccggc atggcgtcct gtatgctcaa gagtatggct gggacgaaca
     1381 tttcgaggcg ctggtcgcac agatcgtggc ggatttcatc aacaactaca accctgcgcg
     1441 agaacgctgt tggatcgcag agatggacgg ggaaatcatt ggttcggttt ttgtcgtgca
     1501 gagtagtgag accgttgcca aactccgcct tctgctggtc gagccgaaag cgcgcggcct
     1561 ggggctgggt actcgcctcg tggaggagtg tattcgtttc gctcgacgcc atgggtacca
     1621 gaagctgatg ctctggacca acagcatcct gctagaagcg cgtcacatct acaagaaaac
     1681 agggtttcaa ctcatcgccg aggaagcgca tcatagcttt ggccatgacc tcgtcggaga
     1741 aacatgggaa ctggttctgt gagcatagga ctttcggcat aaccatttgc tgttttcacg
     1801 cactacagtg ctcatgctcg tttaattacc agagcgaacc acgccagagg gcgagcgtgt
     1861 cgagatggaa catgggaacg ccctcaggtg ttagaagtgt tcgcggtact gtggagtata
     1921 ccggggagga tacattcacg atgcgtagta aaacagcgga actttcgcga gcaatgcaac
     1981 cgcgatggcg cagacggctc tccaccgtcc tttctctgct gctcggcgtc gtcgtagtcc
     2041 tcctgcttgc gtcgctgagc gcgccggtga ctcatgtagc tttcgccaat cctgccggtg
     2101 gcaacctgcc ggtcatccac tgggacgcga gcatgattta cgccgggcag aacaatggct
     2161 atccctgggg tccggtcggc gaaaatacga ttgtgcatgg cgcgaatttc aatccgaata
     2221 ctcagctgca cctgatactc gcgccgggcg atagcaatag caatccggca gtctgccgac
     2281 agccaacagt caccgtactc gtcgcgacag tgactgccga cacgaccggc ggcttcacgc
     2341 aaagtttccc ctggccctcg gcagcaggtc aggtcaatca ggcatatagc atctgtagcc
     2401 tgcggatgtc caataacacc gtcgccagct acctggatga tggtcccttc accgtcctat
     2461 cgtccagtcc cccgcttatc gccctttcgg catccagcgt cgcggcgggc aatgccatca
     2521 ccgtcacagg tcgatactgg gtgccacctc agccagtctc ggttattatc gcttcgtgcg
     2581 ccgactgtgg cggggctgtg cttgttgcga acaccggcgc caattctacc ggactcaata
     2641 gcggctcgtt cagcgtttct atccccattc cggcaacggc aaaaccgggc gattatgtcg
     2701 tcaacgcgct gacgacgacg ggcctggatg ccaactatac cagcggcgtc aagcgcctgg
     2761 ccatcactgc cgcgcccaca acgcagccct cgccaacgcc aacccccacc ttacagccct
     2821 cgccaacgac cagcccgaca gtcacggcca ccgcgacaac tgcggcctcg gcgacgcccg
     2881 ctgcatcggc cacatcgaac ggcgcaagcg gcactgtcac caccacctct aacggctcca
     2941 gtggaacaac cggcactgga acttcgagcg gcaa
//
LOCUS       DASZAK010000062         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_72572, whole genome shotgun sequence.
ACCESSION   DASZAK010000062 DASZAK010000000
VERSION     DASZAK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72572"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(434..724)
                     /locus_tag="VGT44_00975"
     CDS             complement(434..724)
                     /locus_tag="VGT44_00975"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV2579396.1"
                     /translation="MMQAVLLREKREEILRIAKNYGASNVRVFGSVARGEADEKSDID
                     LLVDMERGRSLMSLAGLLVDLEELLECQVDVVPEDSLRERIKSRALQEAVPL"
     gene            complement(810..1856)
                     /locus_tag="VGT44_00980"
     CDS             complement(810..1856)
                     /locus_tag="VGT44_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2579397.1"
                     /translation="MTSMIEADGLSKSFRIARRRTGLLGRLRSVVDPEVRVVEAVRDL
                     SLRIERGEMIGLIGPNGAGKSTTIKMLTGILMPSGGTVRVAGLEPQRQRRELAGRVGV
                     VFGQRSQLWWDLPLIDSLLLLRHLYRVPEGRHAANLARLREMLDLNEFIDTPVRQLSL
                     GQRMRGDLAAALLHDPELLYLDEPTIGLDVVAKARIRAFLQAINAEHGVTVLLTTHDM
                     DDIEMLCKRMLIIDHGRKLYDGTVDDIRLRFGGERTLVAECGPADLAALPRDEAGQPT
                     LLDIPAGVRLAKVEAPRLWLSFQRDALPAHELVAWLGAHCALRDVTFQEPEIEDVIRR
                     IYEEGLLLQDGVIG"
     gene            complement(1859..>2377)
                     /locus_tag="VGT44_00985"
     CDS             complement(1859..>2377)
                     /locus_tag="VGT44_00985"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2579398.1"
                     /translation="RVLLRPVQALILVSSGEFRLRRLGRLTQGVAGFIFALFLLGGIP
                     WTLLKLLALLLGIVSGSLIFLSILLLGATMCFWTVQTAELTNILYYGGREMLSYPISI
                     YNPTIQRILLFVIPLAFGTFLPTCYLLDRPLPLGLPVWIAFLSPPVALAFSAIAVWTW
                     GQGVRHYESTGS"
BASE COUNT          551 a          691 c          655 g          480 t
ORIGIN      
        1 cggaataaac agattttgat aggcttccca gccggtgtaa aaaggggcaa cgttagtgat
       61 ggattcaaac atagcgactt ctcctttgca actggtcata tttctttcgt gtcatctaga
      121 attgcatcca cccgtgcttt caacacgggt aggtcatcct cgaccacagc ccaaatgcga
      181 tcaagattaa tatcaaagta gaggtggata aggatattcc gcatggcatt tatctgcttc
      241 cagggtatat ctggatagat gtctctaaag tcttgaggaa ttgcgcgtac agcttctcca
      301 acaatttcaa gatgacgaat aacccaattt tgtattaact catcttgata gaacagttct
      361 ttaccctggc tggtatattt ataaatatgt acgatggctt cctatacatc gcgcagtcgc
      421 tcataggagt ctctcacagt ggaaccgcct cttgaagcgc tctactctta attcgctcac
      481 gtaagctgtc ctctggaacg acatctacct gacattctaa taattcttcc agatcaacta
      541 acagaccagc cagactcatc agacttcttc cgcgttccat atcgacaagc aaatcaatat
      601 cactcttctc gtcggcttcc ccacgtgcga cagagccaaa aacacgaacg tttgatgcgc
      661 catagttctt cgcgatgcgc aagatttctt cgcgtttttc gcgcagtagt acagcctgca
      721 tcataacgtt cgccctcttt tctagcactc agtttatctc tgcttacata ttaccatagg
      781 caagcgtctt acgaaagtca cacgactgct cacccaatca ccccatcctg taacaacaat
      841 ccttcttcat agatgcggcg aatcacgtct tcgatctccg gctcctggaa ggtgacatcg
      901 cgcagcgcgc agtgggcgcc cagccaggcg acgagttcgt gcgcggggag ggcgtcgcgc
      961 tggaagctga gccagaggcg cggggcctcc acctttgcca ggcgcacacc agcggggata
     1021 tccagcaagg tcggctgtcc cgcttcatcg cgtggcagcg cggccaggtc ggcggggccg
     1081 cattcggcga ccagggtgcg ctcgccccca aaacggagac gaatgtcatc aacggtgcca
     1141 tcgtagagtt tgcgtccgtg atcgatgatg agcatgcgtt tgcagagcat ctcgatatca
     1201 tccatatcat gcgtcgtcag caagacggta acgccgtgct cggcgttgat ggcttgcaag
     1261 aaggcgcgaa tgcgcgcttt ggcgacgaca tccaggccaa tcgtcggctc gtccaggtaa
     1321 agcagttcgg ggtcgtgcag cagggcagcc gcgaggtcgc cgcgcatccg ctgacccaga
     1381 gaaagctggc gcacgggggt atcgatgaac tcgttcaagt cgagcatctc gcgcaggcgg
     1441 gcaagattgg cggcgtgacg cccctccggc acgcggtaga ggtggcgcag cagcagcagc
     1501 gagtcgatca ggggcaggtc ccaccagagc tgcgagcgct ggccaaaaac aacgcccacg
     1561 cggcccgcca gttcccggcg ctggcgctgc ggttcgagac ccgcaacgcg cacagtaccg
     1621 ccgctgggca tcaggatgcc ggtcagcatt ttaatcgtgg tggatttccc cgcgccgttc
     1681 ggcccgatca gcccgatcat ttcacctcgt tcgatgcgca gcgataaatc tcgcaccgct
     1741 tcgacgacgc gcacttctgg atcaacgaca ctgcgcagcc tgcccagcag accagtgcgt
     1801 cgtctggcta tacgaaacga tttgctgagg ccatcggcct cgatcatgct tgtcattgtt
     1861 agctccctgt actttcatag tggcgcacgc cctggcccca cgtccagaca gcgatggcgg
     1921 agaaggccag cgcgacgggc ggcgagagaa acgcaatcca gacaggcaaa ccgagcggca
     1981 agggacggtc cagcaggtag caggtgggaa gaaatgtgcc aaaggccagc gggataacga
     2041 acaaaaggat gcgctgtatg gtcggattgt agatgctgat gggatagctc aacatctcgc
     2101 gcccgccgta gtagagaatg ttggtcaact cggcggtctg caccgtccag aagcacatcg
     2161 tcgcgccaag cagcaggatg gagaggaaga tcaaggagcc gctgacaatg ccgagcagca
     2221 gcgccagcag cttgagcagc gtccagggaa tgccgcccag caggaacagc gcgaagatga
     2281 agcccgccac gccctgcgtc aggcgaccca ggcggcgcag gcgaaattcg ccgctactta
     2341 ccagaatgag cgcctgcacc gggcgcagca gcacacg
//
LOCUS       DASZAK010000063         5982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_1561, whole genome shotgun sequence.
ACCESSION   DASZAK010000063 DASZAK010000000
VERSION     DASZAK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5982
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1561"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1049)
                     /locus_tag="VGT44_00990"
     CDS             complement(<1..1049)
                     /locus_tag="VGT44_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEV2579399.1"
                     /translation="MTALQDLAYKNANVAHLFTQVLDEEVGPLRTELKAVEDRLSGEA
                     KQIIARLEAEIRKQDAEMKELKKLMNRPGAVVGFDSPEAKAEASRSAFNKALKKGWGA
                     LSADERKFVRHDEDFGIASGDQVGHGQDIEVKALYGVDNTTGGFLTVPEYVNELIEAI
                     VLVSDMHDLVNVRMTANPYVKIPKRVQTASAARIVEQATRNETQNPRFGMVQVFPYES
                     YALTLVSRTDMDDSELDLGTFVMSEFSTQFAKLEGYEIINGQGAGANQCLGFLNDPNI
                     TTTQYSGSGGGNGYITSSSSGTFGYPDLVTDVHSLKTGYRKGAAWVFTTETLGAIRKL
                     TDSQNRPLWGPMGSEL"
     gene            complement(1090..2802)
                     /locus_tag="VGT44_00995"
     CDS             complement(1090..2802)
                     /locus_tag="VGT44_00995"
                     /inference="COORDINATES: protein motif:HMM:NF016471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEV2579400.1"
                     /translation="MTTAPATFPSYSIKGRQVEYKSFAFRVKSFDEAKGTITGYLSTF
                     GNIDLQKDCVVRGAFKKTLTEAYARKANGRQYLFALLWMHDPERPIGGVIDAQEDAEG
                     LLITAQLDISVNAAGFPSNPVATMVFSGFKSGYIDELSMGYLAIQKDYEAGIRYLKEV
                     ALLEGSAVTMLMAANPQALVPVTGVKHMLGTKAACGKTTWPLADRKLAWDNGEAHNAI
                     VEWATQDDGSIDQAKLQSVHFWCDESAPENIASYKFLFCDVFDGEIKAVPRGIFACVG
                     GHGLAVADIPESDVPGVQAKISAYYKRMRQEFDDDSIVPTWEESDKAARMPRFVKDFN
                     AVHQAGKAADCLEDWCDLLDDLTQTMFQIFGMGDSPEPDMADCLKQFGAALTNEWLPK
                     ALEADLGQYLNDRGYCNDANSVWIPGSMQVGGYDYGYMARAGRASRKSGRTISAATQE
                     KLEQHQAEMKALMDEHQNQSEQLASQFKAMVKKSMEAMNQKVSDLTDLWADEGQGPAY
                     GNDDPPDVPNHTNDDNGKSRFVRREPTRTPTRSLPRQEEQPPRKSRDTASSITLGDLE
                     ALIV"
     gene            complement(2799..5195)
                     /locus_tag="VGT44_01000"
     CDS             complement(2799..5195)
                     /locus_tag="VGT44_01000"
                     /inference="COORDINATES: protein motif:HMM:NF016732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV2579401.1"
                     /translation="MGFFNWARGILQRKADPTYQVEAQFFSPGAPIWSNKDYRTYVSQ
                     GYRRCGTVYNCVNKISGAASGIKWKLYTDRSMSREITSHPLLDLWNKPNPRMGASSFV
                     EQCFGHWHLSGNAYLYASRPSPGAPPVELWPLRPDLIKIVAGEKEVAGYVYGWGTSRA
                     QDFDVDQIMHLKFPAYDDDFYGLSPVEVAMQLVDQQNEGNSWNTALMQNAGKPASVFF
                     SKGYLTLEQRTQVRQELLKRYSGKRNAGMPMVLEADMTWQSMSMSPYELDWLQSRELN
                     TREIAAIFDVAPELVGDSAGKTFANQKEAKQSLYTENVLPKLDRFDGYLNVWLVPMFA
                     DLAASGAWFTYDRSDIEVLAELYTAQQQALSEQANSMWNSGQCSLRVAQRLQGLPARP
                     KVYLDVYKFGAILVREEDLEAYAMQALQKPAAPPSPVPEPLLNQPSSLPQLPAPKPAA
                     RTTVTEVPNDEQQNDQNPDGGAAPATGKEKRSHAAPEYKLLDLATREAKEAYAKQVEA
                     DRQAWEEKIEARFQAYFRAEQKAVVAAIRAASIESEAADGIENAISGLKDDLQQQLYN
                     CWYDVGRDFGGRAQAQFAEASKASESAYSRKTTPDLAALFTAQTIAYLLNLAGTKVQQ
                     ISGTTLTDLRQALATGVAAGESIPELAKRIDQLYLTQIIPNRSTNIAQSEVCAASNWA
                     SHQAASQSGLTLNKVWLATEDSRTWPDHADADGQKVALDQPFTVGGEQMMFPGDSSMG
                     ASAKEICRCRCTQYYERADATTGTQDEERDGEAGDEKVIRLIPRRSYRSVREFMEAMA
                     "
     gene            complement(5182..5334)
                     /locus_tag="VGT44_01005"
     CDS             complement(5182..5334)
                     /locus_tag="VGT44_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579402.1"
                     /translation="MQKRKITRKERQAQHQLVQERVKAIQTEQTFLRATVALLKKKEA
                     EHHGLL"
     gene            complement(5334..>5982)
                     /locus_tag="VGT44_01010"
     CDS             complement(5334..>5982)
                     /locus_tag="VGT44_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579403.1"
                     /translation="GLLDQTLRALPGISAFVLKDGNGQYLHYFNRWIVRVQGDILFFR
                     FALEHQGYGTIEGYLNERDVQEIEQQAKRRYSIPLREYRPVRDKVSRASSAAILMENG
                     KLYFLASLGDLPEIKAELLRFPKAANDDIVDCASQAAEVVLVPAGPMLWSADSEDLPP
                     TAVHPLPSSLQERRSPESAPASDRPHLMLTIDDPENTWQPISPDEGFMVDWEVPG"
BASE COUNT         1128 a         1819 c         1673 g         1362 t
ORIGIN      
        1 agttcgctac ccatcggccc ccacaacggg cgattctgcg agtcagtcag cttcctgatc
       61 gcgcccaacg tctcggtcgt gaagacccag gccgccccct tacgatatcc agtcttgagc
      121 gagtggacgt ccgtcaccag gtctgggtag ccgaaggtgc cagagctaga gctcgtgata
      181 tagccgttgc ccccgccaga gccactgtac tgcgtggtgg tgatgttcgg atcgttcaaa
      241 aaccccaggc actggttcgc gccagcaccc tgcccgttga tgatctcgta cccttccagc
      301 ttcgcgaact gggtgctgaa ctcgctcatc acgaacgttc cgagatccag ttcgctgtca
      361 tccatgtcag tgcggctgac cagggtcaac gcatagctct cgtacggaaa gacctggacc
      421 atgccaaaac gggggttctg ggtctcgtta cgcgtcgcct gctcgacgat gcgagccgcg
      481 ctagcagtct gcacacgttt cgggatcttg acatagggat tcgcggtcat acgcacgttc
      541 accagatcgt gcatatccga aacgagcacg atcgcctcga tcagctcgtt cacgtactct
      601 gggacggtca aaaagccgcc agtggtgtta tcgacgccgt agagcgcctt gacctcgatg
      661 tcctggccgt gaccaacctg gtcgccgctc gcgatcccaa agtcctcatc gtggcgcacg
      721 aatttgcgct cgtccgccga gagcgcgccc caccctttct tcaaagcttt gttaaaggcg
      781 gaacgcgagg cctcggcttt cgcctccggg ctgtcgaagc cgacgaccgc cccaggccgg
      841 ttcatgagct tcttcagctc tttcatctcc gcgtcctgtt tgcggatctc cgcttccagg
      901 cgcgcaatga tctgcttcgc ttcgccggaa agacggtctt caactgcttt gagttcggtg
      961 cgcagcgggc cgacttcttc atccagcacc tgcgtaaaca gatgtgcgac attggcgttc
     1021 ttatacgcca gatcttgtaa cgctgtcatt gggtggtatt ccttgggaga aagttgcgaa
     1081 ggaccgcaac tacacaatca aagcttccag atcgccaagc gtgatcgagc tcgcagtgtc
     1141 gcgactcttg cgcggcggct gctcttcctg gcgtgggagt gaccgtgttg gtgtgcgagt
     1201 cggctcccgg cgcacaaagc gcgattttcc gttgtcgtcg ttggtatgat taggcacgtc
     1261 gggcggatcg tcattgccat aggcaggacc ctgcccttcg tctgcccaga gatcggttaa
     1321 atcactgacc ttctgattca tggcctccat gctctttttg accatggctt taaactggct
     1381 ggctaattgt tcagactggt tctggtgctc gtccataagc gccttcatct ccgcctggtg
     1441 ctgctctagt ttttcctgtg tggccgcgct gatagtccgg cctgacttcc ggctggcgcg
     1501 accagcgcgc gccatgtaac cgtaatcata gccaccgacc tgcatactgc cagggatcca
     1561 aacactgttt gcatcgttgc aatagccacg atcattcaaa tactgcccca agtcagcctc
     1621 cagggctttg ggcagccatt cattggtgag cgcagcgcca aactgcttga ggcaatccgc
     1681 catatccggc tctggtgagt cgcccatacc gaaaatctgg aacatggttt gcgtcaggtc
     1741 atctaacaga tcgcaccaat cttccagaca atccgcagcc ttccccgcct gatggacggc
     1801 attgaaatct ttcacgaacc gaggcattcg cgcagccttg tcgctctctt cccaggttgg
     1861 cacaatgctg tcgtcgtcaa actcctgacg catgcgtttg tagtaggcgc taatcttcgc
     1921 ctgcacgcca ggaacgtcgc tctctgggat atccgcgacc gccagaccat ggccgccaac
     1981 gcaggcaaag atgccgcgcg gcacggcctt aatctctcca tcaaagacat cacagaacag
     2041 gaatttgtac gaggcgatgt tctctggtgc gctttcgtcg caccagaagt ggacagattg
     2101 cagtttcgcc tgatcgatgc tgccatcatc ctgagtggcc cactcgacaa tcgcgttatg
     2161 cgcctcgccg ttgtcccagg cgagtttccg atcagcgagc ggccaggtcg tcttgccgca
     2221 ggcggctttg gttccaagca tgtgtttgac tcctgttaca ggcacaaggg cctgcggatt
     2281 ggccgccatg agcatggtga ccgccgagcc ctcaaggaga gcgacttctt taagatagcg
     2341 gatgcccgct tcatagtcct tctggatcgc caggtacccc atggataact catcaatgta
     2401 gccggactta aagccgctga aaaccatcgt ggcgacagga ttgctgggga agccggcggc
     2461 attcacgctg atgtcgagct gtgccgtgat cagcaaccct tcggcatcct cctgagcatc
     2521 aatgacgccg ccaattgggc gctccgggtc atgcatccag agaagcgcaa acagatactg
     2581 gcgaccgttg gctttgcgcg cataggcttc ggtgagggtc ttcttaaacg cgccgcgaac
     2641 cacacagtcc ttctgcagat cgatattgcc aaacgtcgaa agatagccgg tgatcgtccc
     2701 tttcgcttcg tcgaaggact tgacacggaa cgcaaacgat ttgtattcca cctggcggcc
     2761 tttgatgcta taactcggga atgtcgcggg tgctgtcgtc atgccatcgc ctccatgaat
     2821 tcgcgaacac tgcgatagct gcgtcgagga atcagcctga tcaccttctc gtcgcccgct
     2881 tctccgtcgc gttcctcgtc ctgggtgccg gtagtggcat cggcccgctc gtagtattgg
     2941 gtgcaacggc agcgacaaat ctccttcgca gacgccccca tggagctatc accagggaac
     3001 atcatctgct caccgccaac cgtaaatggc tgatccagcg cgaccttctg tccgtcagcg
     3061 tctgcatgat caggccaagt cctgctatcc tccgtcgcca accacacttt gtttagcgtg
     3121 agcccgctct ggctggccgc ctggtggctt gcccagttgc tggcagcaca cacttcgctc
     3181 tgggcgatat ttgttgagcg gttggggatg atttgcgtga gatagagctg atcaatccgc
     3241 ttcgccagct caggaatgct ctcccctgcc gcaaccccgg tagccagcgc ctgtcgcaaa
     3301 tcggtcaggg tcgtgccact aatttgttgg acttttgtcc cggccagatt gagcagatac
     3361 gcgatggtct gtgcggtaaa gagtgccgca agatcaggcg tggtcttcct gctgtatgcg
     3421 ctctcagacg ctttgctggc ctcggcaaat tgcgcttgcg cgcgaccacc aaagtcgcga
     3481 cccacgtcat accagcagtt gtaaagctgc tgctggagat cgtctttcag gcccgagatc
     3541 gcattctcaa tcccgtcagc ggcttcgctc tcgatggagg ctgcgcgtat ggccgcgacg
     3601 accgcttttt gctccgcacg gaaatacgct tggaagcgcg cctcgatctt ctcctcccaa
     3661 gcctgccgat cggcttctac ttgcttcgcg taggcttctt tggcttctcg ggtcgcgagg
     3721 tcgaggagct tgtattcagg cgcggcgtgg ctacgcttct ctttgccggt cgcgggtgcc
     3781 gctccaccgt ctgggttctg gtcgttttgt tgttcatcgt tgggaacctc ggtcacggtc
     3841 gtcctggctg ccggtttggg tgcaggcagt tgcgggagag acgagggctg attaagcagc
     3901 ggctctggca ctggcgacgg cggagctgcg ggcttctgta acgcttgcat cgcgtaggct
     3961 tccaggtcct cttcgcgcac gaggatggca ccgaacttgt acacatccaa atagaccttc
     4021 gggcgcgcgg ggagtccttg cagtcgctgg gccacacgca agctacactg gccgctattc
     4081 cacatcgaat tcgcttgctc agagagcgcc tgctgctgtg cggtataaag ctcggcgagg
     4141 acctcgatat cggagcgatc gtaggtaaac caggccccgc tcgcggccaa gtccgcgaac
     4201 atcggcacca gccagacgtt gaggtatccg tcaaatcgat cgagcttcgg caggacattc
     4261 tcggtgtaga gcgactgctt cgcttctttc tggttcgcaa aggtctttcc agcagaatcg
     4321 ccgacgagct ctggcgcaac gtcgaagatc gccgcgatct ccctggtgtt tagctcgcgt
     4381 gattgcaacc agtcaagctc atagggactc atcgacatcg actgccaggt catatcggct
     4441 tccaagacca tcggcatccc agcattgcgc ttcccagagt agcgcttgag cagctcctgt
     4501 ctgacctgcg tacgttgctc cagggtcaga taccctttcg agaagaacac gctggcaggc
     4561 ttgcccgcat tctgcatcag cgccgtgttc cagctatttc cttcgttttg ctggtcaacc
     4621 agttgcatgg cgacttccac tggcgacagg ccatagaaat cgtcatcata cgccgggaac
     4681 ttgagatgca taatctgatc cacgtcgaag tcttgcgccc ggctcgttcc ccagccatag
     4741 acatagcccg caacctcttt ctcgcccgca acgatcttga tcaggtccgg gcgcagtggc
     4801 cacaactcca ctggcggcgc acccggcgaa ggacggctgg cgtagagata cgcgttgccg
     4861 gaaagatgcc agtgtccgaa gcattgctca acaaaagagc tggctcccat gcgtggattc
     4921 ggcttgttcc agagatcgag tagtggatgg gaggtgatct cccggctcat gctccgatcc
     4981 gtgtagagct tccacttgat gccgctcgct gctccgctga tcttgttgac acagttgtag
     5041 acggtgccac agcgccgata gccctggctc acatacgttc ggtagtcctt gttggaccat
     5101 ataggcgcgc cagggctgaa gaactgtgcc tcgacctggt aggttgggtc ggctttgcgc
     5161 tgcaggatcc cgcgcgccca gttaaagaag cccatggtgt tcggcctcct tcttcttgag
     5221 cagcgcgacg gttgcacgca agaacgtctg ctcggtctgg atcgctttga cgcgttcctg
     5281 gacaagctgg tgctgagctt gccgttcttt ccgggtaatc ttgcgcttct gcatcagcca
     5341 ggtacctccc agtccaccat aaagccctcg tccgggctga tcggctgcca ggtattctca
     5401 ggatcgtcga tagtcagcat gagatgcggc cggtcagatg caggagcgct ctccggcgac
     5461 cgtcgttcct gcagagagct gggcaacggg tgaaccgccg ttgggggcaa atcctcagag
     5521 tccgctgacc acaacatcgg ccccgctggg accaggacca cttctgccgc ctggcttgcg
     5581 cagtccacaa tatcgtcatt ggcggccttg gggaagcgta gcagctccgc cttgatctct
     5641 gggaggtcgc ccaggctcgc caggaaatac agcttgccat tctccatcag aatggcagcc
     5701 gacgaggcgc gcgaaacttt atcccgcact ggtcgatact cgcggagcgg gatggaatag
     5761 cgccgcttgg cctgctgctc aatctcctgg acatcgcgct cattgagata accctcgatg
     5821 gtcccgtagc cctggtgttc cagggcaaag cggaagaaga gaatatctcc ctggacgcgt
     5881 acaatccagc gattgaaata gtgcaggtac tggccattgc cgtctttcaa cacaaaggcg
     5941 gagatccctg gcagcgcacg cagggtctgg tcgagcagac ct
//
LOCUS       DASZAK010000064         2060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_427611, whole genome shotgun sequence.
ACCESSION   DASZAK010000064 DASZAK010000000
VERSION     DASZAK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427611"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VGT44_01015"
     CDS             <1..241
                     /locus_tag="VGT44_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579404.1"
                     /translation="AHVVGQSVSCTYAARMVAWMRVGLPQGNFVDTIAHRRVVHKGGA
                     GSASLLAHHRVCNRDMLPTRKRRGFSGYARRTRPR"
     gene            complement(209..829)
                     /locus_tag="VGT44_01020"
     CDS             complement(209..829)
                     /locus_tag="VGT44_01020"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2579405.1"
                     /translation="MPAQPLPSTGRETGIDVGLKVFLIVADGEMVENPRHYRKAERRL
                     AKAQRRVSRRVKGSQRRRKAVRLLARAHQQVRRARADFHHKAALALLRQYDTIYLEEL
                     QVRNLVRNPHLAKSISDAGWGAFRTILDAKAACAGRHVIAVPAQYTSQDCSGCGERVP
                     KSLLVRTHVCPSCGLVIDRDENAALNILRAGQARQASTWPGAASVA"
     gene            complement(851..1351)
                     /locus_tag="VGT44_01025"
     CDS             complement(851..1351)
                     /locus_tag="VGT44_01025"
                     /inference="COORDINATES: protein motif:HMM:NF023742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2579406.1"
                     /translation="MREGVRKTFKYQLRPTPDQERLLDRTLMRCRHVYNAALEQRRTW
                     WGRGQGKGATYYQQKAELPDLKAACPEYAEVHAHVLQDVILRVERSYQAFLRRVKNGE
                     TPGYPRFQGRVRYNSFTFPEYGNGAVLDGGILSRSQIGRLRIRLHRPLEGTPKTVTIS
                     QEADGW"
     gene            complement(1354..1518)
                     /locus_tag="VGT44_01030"
     CDS             complement(1354..1518)
                     /locus_tag="VGT44_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEV2579407.1"
                     /translation="MADQQTHHKITLSIPRETWEAVNELAREHQRSFTKELVWALQEY
                     VRRERKRRER"
     gene            1829..>2060
                     /locus_tag="VGT44_01035"
     CDS             1829..>2060
                     /locus_tag="VGT44_01035"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV2579408.1"
                     /translation="MREVVPESPADLAGIQPGDLIKSINGNPVRDLVDYQFYASEDEL
                     QLGVERNGRAIDVLMAKEPDEETGIRFGEEPAP"
BASE COUNT          371 a          670 c          634 g          385 t
ORIGIN      
        1 ggcgcatgtt gtaggccaga gcgtctcgtg cacctatgcc gccaggatgg tagcgtggat
       61 gcgcgtgggc ttaccccagg gaaattttgt tgacacgata gcacacaggc gtgtcgtcca
      121 caagggagga gcaggaagcg cctcgctgct ggcacatcac cgcgtatgca accgtgacat
      181 gctccccacg cgtaaacgca ggggcttctc aggctacgct cgccgcaccc ggccacgttg
      241 acgcctgacg ggcctgcccg gcccggagaa tgttgagtgc cgcgttctcg tcgcggtcta
      301 tcaccagccc acaggaggga cagacatggg tacgcaccaa caggcttttt ggcacgcgct
      361 ccccacaccc actacagtcc tggctggtgt actgcgcagg caccgcgatc acgtgacgcc
      421 cggcgcatgc tgccttggcg tcaaggatgg tgcggaacgc tccccagccg gcatcactga
      481 tgctcttggc caggtggggg ttgcgcacca gattgcggac ctgcaactct tccagataga
      541 tggtgtcgta ctgccgcagc agcgcgagcg cggccttgtg atggaagtcg gcccgcgccc
      601 gacgcacctg ctgatgcgct cgcgccagca atcgcaccgc cttgcggcgg cgctggctgc
      661 ccttcacccg gcgcgacacc cgacgctgcg ccttggcaag tcgtcgctca gccttgcggt
      721 agtggcgcgg gttctctacc atttcgccgt ctgccacgat caggaacacc ttcaagccca
      781 catcaatgcc ggtctcgcgt ccggtagagg gcaaaggctg tgctggcacg tcggcacagg
      841 agatgcaggc ctaccagccg tctgcctcct ggctgatcgt caccgtcttg ggcgtgccct
      901 ccagcggacg atgcagccgg atacggaggc gcccaatctg cgagcggctg agtatcccac
      961 catccagcac cgcgccgttg ccatattcag ggaacgtgaa gctgttgtac cggacacgtc
     1021 cctggaaccg gggatagccc ggcgtttccc cgttcttcac ccgccgcaag aaggcttgat
     1081 agctccgctc gacccgcaag atcacgtctt gcaaaacgtg cgcgtggacc tcagcatatt
     1141 ctgggcaggc cgctttcagg tccggcagct ccgccttctg ctgatagtag gtggcgccct
     1201 tgccctgccc gcgcccccac cacgtgcggc gctgttcgag cgcggcgttg tagacatggc
     1261 gacagcgcat cagcgtacgg tcaagcagcc gctcctggtc aggagtgggc ctgagttggt
     1321 acttgaacgt cttgcgcacg ccctcgcgca tgattatcgc tctcgcctct tgcgctcccg
     1381 acgcacgtac tcttgcagcg cccacaccaa ctctttcgtg aacgagcgtt ggtgctcgcg
     1441 tgccaattcg ttcaccgcct cccacgtctc acgcgggatg ctgagcgtaa ttttgtggtg
     1501 cgtctgttgg tcggccatac tggaagtgta ccataactat tccggtattg caagcggata
     1561 ggacatacga cgccctgcgg gcgacagcgc ccttacccca tagctaaagc cagggggctg
     1621 cgggcgctct ccgctcagtg agcaaacggc aatgccagtg agccaatctg gcttggaata
     1681 tagcatactg gagtcgcagt tgggcggcac gagcgttcca ggtttgacgt actctccggc
     1741 caaagcctat actccgtagg ttggccgcga ataacaccac tgaaatgcgt cgggacatca
     1801 cgagaccatg ccaagactct atgggtgggt gcgcgaagtc gtgccggaga gccccgccga
     1861 ccttgccggc atccagccgg gcgacctgat caagagtatc aatggcaacc ccgtgcgcga
     1921 cctggtggat taccagttct atgcctccga ggacgagctg cagctgggcg tcgagcgcaa
     1981 cggccgggca atcgacgtgc tgatggcgaa ggagccggac gaggagacgg gcatccgctt
     2041 cggcgaggag ccggcgccct
//
LOCUS       DASZAK010000065         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_640667, whole genome shotgun sequence.
ACCESSION   DASZAK010000065 DASZAK010000000
VERSION     DASZAK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_640667"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..802)
                     /locus_tag="VGT44_01040"
     CDS             complement(95..802)
                     /locus_tag="VGT44_01040"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEV2579409.1"
                     /translation="MTVFRYHPDILARYPGVAGGVILARGMTNGPTPKDLQAAFQAEQ
                     QATLQRIGTTPLSQVESLAAWRSAFRSFDVDPTQYRSAAEALLRRLTKKGDIPSINLL
                     VDLGNLVSIRYALPVAVFDTRALQGAVTVHFADGSERYTTLGEDEVDHPEPGEVVFSD
                     ETKLVIARRWCWRQSDQSAAQEDTRQAIITVEAHHANGQRDVDAALKDLLELLRRYAG
                     GNFIWGRLGTTNPAISD"
     gene            complement(812..2038)
                     /gene="trpB"
                     /locus_tag="VGT44_01045"
     CDS             complement(812..2038)
                     /gene="trpB"
                     /locus_tag="VGT44_01045"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV2579410.1"
                     /translation="MNYRETYQHHLYPDERGRYGAFGGKFVPESLMAALADLEAAYEE
                     ARSDDEFQSELQAQLASFVGRPTTLHHVPRFSQMVVPGIKVYLKREDLAHTGAHKINN
                     ALGQGLLAKRMGKRRIIAETGAGQHGVATATVCAMLGLECIVYMGEEDIGRQSLNVFR
                     MKLLGAEVRAVTSGSRTLKDAINEAIRDWVTHVETTFYLIGSAVGPHPYPRIVRDFQS
                     VIGSETREQILLKEGRLPDMVVACVGGGSNAIGMFYPFAEFPDVQLIGVEAGGSSLEP
                     GRHAATLVAGRPGVLHGAFSYLLQNEDGQILTTHSISAGLDYPGVGPEHSYLKETGRA
                     TYVSASDTCALRGLQALCQSEGIIPALEPAHALGYLLEMGEQGKIPPGSLIVLNLSGR
                     GDKDMLTVAKALHVTL"
BASE COUNT          484 a          729 c          642 g          564 t
ORIGIN      
        1 atgttctgtc aagcttgaaa tgtagtggca tcccttgcgg atgccaggtc ctcactcatc
       61 aaggtcctca cccattcaat gaacctgcta gtagtcagtc cgagattgcc ggatttgtcg
      121 taccaagcct tccccagata aagtttccac cggcatatct gcgcagtaat tcaagtaaat
      181 ctttgagagc agcgtcaaca tctctttggc cattggcatg gtgcgcttca acagtaataa
      241 tggcctgcct ggtatcctct tgcgccgcgc tctggtcgct ttgtcgccag caccagcggc
      301 gcgcgatgac cagcttcgtt tcatcagaaa aaactacttc gcccggttct ggatgatcca
      361 cttcgtcctc gcccagcgtg gtatagcgtt ctgaaccgtc ggcaaaatgg actgtgactg
      421 ctccctgcaa ggcgcgtgta tcaaacactg cgacgggcaa tgcatagcga atactcacca
      481 ggttgcccag gtcaaccagc aagttgatgc tcggaatatc ccctttttta gtcaaacggc
      541 gcagcagcgc ttcggctgcg ctgcggtact gtgttgggtc tacgtcaaaa ctgcggaaag
      601 cgctgcgcca cgccgccagt gattcgacct ggctcaaagg agtagtcccg atgcgttgta
      661 aggtagcctg ctgttcagcc tggaaagccg cttgcaaatc tttcggtgtc gggccattag
      721 tcatgccccg cgccaggatc acaccgccag ctacacctgg ataacgggcc agaatatcag
      781 gatgatagcg gaaaactgtc atctttcact cctacaacgt aacgtgtaac gctttcgcga
      841 cagtaagcat atctttgtca ccgcgccccg acaggttcag cacgatcagt gaaccaggcg
      901 gaattttgcc ctgctcgccc atctccagca ggtagcccag cgcatgcgca ggctcaagtg
      961 ccgggataat gccctcgctc tggcacaacg cctgcaaacc acgcagtgcg catgtatcgc
     1021 tcgctgagac ataggtggcg cgtcccgtct ctttcaggta gctgtgctct ggtcctacgc
     1081 cgggatagtc aagacccgcc gagatgctgt gggtagtcag tatttgccca tcttcattct
     1141 gaaggaggta gctgaatgcg ccgtgtagta cccctggcct gccggccacc agcgttgccg
     1201 cgtgccgtcc tggctcgaga cttgagccgc cggcttctac gccaatcagc tgtacgtcgg
     1261 gaaactcagc aaagggatag aacataccaa tggcgttgct gccaccgccg acgcaggcaa
     1321 caaccatatc gggtagccgt ccctccttta aaaggatctg ttcgcgtgtc tcactgccga
     1381 tcaccgattg aaaatcgcgc acgatgcgtg ggtagggatg cggtccaact gcggagccga
     1441 tcaggtagaa ggtcgtctca acgtgggtta cccagtcccg gatggcttca ttgatcgcat
     1501 ctttaagggt gcggctgccg ctggtcaccg cgcgtacctc ggcgcccagc aatttcatgc
     1561 ggaaaacatt gagagactgg cgcccgatgt cctcctcacc catgtacacg atgcattcga
     1621 gacccagcat agcgcagaca gtcgcggtgg caacgccatg ctgcccagcg cctgtttcag
     1681 caatgatgcg ccgcttgccc atacgttttg ccagtaaccc ctgccccagc gcgttattga
     1741 ttttgtgtgc gccggtgtgt gccaggtctt cgcgcttgag gtagaccttg atacccggca
     1801 ctaccatctg ggagaaacgc ggaacatgat gcagcgtggt tggccggcca acgaagctgg
     1861 caagttgcgc ctgcagttct gattggaatt cgtcgtcgct gcgtgcctct tcgtaggctg
     1921 cttctaagtc agcaagcgct gccatcaggc tttcaggtac gaatttcccc ccgaatgctc
     1981 cgtagcgccc acgctcatcg ggatagagat gatgctgata tgtttctcta taattcatgc
     2041 tgacattact cttctttccc gtactcgctg aacgaacgcc cgtatcttgc cggcatcctt
     2101 aactttgttg gtctcaacac cgctgcttac atctactccc caggggcgaa cctgctcaat
     2161 tgcctccgcc acgttctcag gcgttaaccc ccctgccagc agtacaggca tgtgttgtgc
     2221 tactgaccgg gcccgctccc agtcgtgcgt ttctcccgtt cccccccatt ggcgctccat
     2281 gtccgcgggg gaaataccct ccccgtaggg gcgagggtgg tgaagggtgg ttgggcaaga
     2341 aagatacggg gtatctttac aaggctttat ggtcgcccgc cgtcccccta ccccccattt
     2401 cggtgttggc gtgtcgagt
//
LOCUS       DASZAK010000066         4835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_356638, whole genome shotgun sequence.
ACCESSION   DASZAK010000066 DASZAK010000000
VERSION     DASZAK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356638"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VGT44_01050"
     CDS             complement(<1..941)
                     /locus_tag="VGT44_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2579411.1"
                     /translation="MEDQQTFPSSFGELLKTRRKRKRLTQKQLAQQLGVHANTISSWE
                     LGTYLPDTRGLVLELARRLALDEHETRQLLEASLTALSPHWLVPLARNPFFTGREEIL
                     EALHTQLHVEQVVALTQSYALRGLGGIGKTQIALEYAYRHALEYSAIFWIDAETIEHV
                     MSSLLRIAELLQLPERQEADQQRIVAAVQRWLSTHSQWLLIWDNVEDLEMLQRLLPPT
                     RQGATLITTRRQALGTLARGMDLVPMEREEGMLFVLRRAKVLEPEATSERMHQLALSM
                     PAEYAATEKLVAAMEGIPLALDQAGAYIEETGCSVSA"
     gene            1342..3057
                     /locus_tag="VGT44_01055"
     CDS             1342..3057
                     /locus_tag="VGT44_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2579412.1"
                     /translation="MLKQARKSRFLMMSFSLLATFILILSACGGGATTSTTTSTGAPV
                     KGGTWIDDLYEEPDSLLPNMSSETFATIVMDGLYAPLIYGTPQGQLMPGIATSVPTVQ
                     NGGVSADLKTVTFNMRPGLVWSDGKPLDARDVDYSWKLWANPKAAAYNTVFAQDIASA
                     DITNNNLTITFHLKNPFVSFVSQWADGGLGAPLPAHIFSSMDPAAVLRSPQNLNPTAT
                     SGPFMMSESKPGDHYTIVRNPKYYLASQGLPYLDSVVFRIVADQNTILKDLQAGSIDS
                     AWFLDVSKAVAYQSLSNYQIIGDGGGTGTNAYSWEAIWINFNNKILGGNPEVRQAMAM
                     AVDQQSLIQVARRGFAGIRCQDHPSLQVPGYTTSLACPKFDLTAANALLDQHGWVKGS
                     DGVRSRNGQRLEFQYSTTAGNLWRSDDQLLNQANFMKIGIKVDIMNYPASTFFGSVLP
                     AGKPGQYDIAEFAESGAFDPDNSILLTCAEKGLGNFAFYCASQMETLQKQQLSTGDNT
                     ARQTAFDGINQLEVTDTPFIVEFSAPDLAVYKKGTHNYLPSAVGVGSTENIWLWWCDN
                     GTCPA"
     gene            3241..4203
                     /locus_tag="VGT44_01060"
     CDS             3241..4203
                     /locus_tag="VGT44_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579413.1"
                     /translation="MGRYIIRRIIQAIPLLILLSVAMFGLIHLLPGGADAVLFNPRLT
                     AQARAGLRARMGLDDPFYIQYFKWISSALVGNFGYSFNTNESVSSILARRFPATLELF
                     VTAFVVALTLAILIGVISAVRQGTLTDYSLTILAYIGISMPAFLLGLFLQDIFGVWLK
                     WLPTSGTGSYGVAFSLYQSLVDHLEHFILPVTALSILFIAGWSRYLRSSMIEVVKQDY
                     MRTARAKGVDSRTLLLRHALRNAVIPMITIVALDFGAIAGGATITEGVFAWPGMGLLF
                     IDSLDRRDYPVLIAMLMLSAFFVIAFNLIADILYAVLDPRIRYS"
     gene            4243..>4835
                     /locus_tag="VGT44_01065"
     CDS             4243..>4835
                     /locus_tag="VGT44_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579414.1"
                     /translation="MDISEDLNATSTTVEEVDPFLELAPALVQKRRSQGRIIFDRFIR
                     NKAAIGGAIFLILLFVFCSLGPLFWQVDPKQQNILSISETLAPPSLAHPLGTDDVGRD
                     TLARVMAGGRVSLSVGLTSMLMAILFGIGVGAIAGYYGGVMDNVLMRATDVVLAIPLY
                     LLLFVLSASFTDGTPKSVVILIAFFGWTYAARLARGE"
BASE COUNT          948 a         1362 c         1390 g         1135 t
ORIGIN      
        1 taagcggaca cactacaccc cgtttcctcg atataagctc ccgcctggtc gagggccagg
       61 ggaatcccct ccatggctgc caccagtttt tctgtcgcgg catactcagc gggcatgctc
      121 aaggcaagct ggtgcatccg ctcgctcgta gcttctggct cgagcacttt ggcccgtctc
      181 aatacgaata acatcccttc ttctcgctcc attggcacaa ggtccattcc ccgcgccagc
      241 gttccgagcg cctggcgtcg tgtagtgatc aaggtcgcgc cctgccgggt aggcggcagc
      301 aggcgttgca acatctccag gtcttccacg ttatcccaga tcagcagcca ctggctatgg
      361 gtgctcagcc agcgctgcac cgccgccacg atgcgctgct gatccgcttc ttgtcgctcg
      421 ggtaactgta atagctcggc gatgcgcagc aggctcgaca tgacatgttc gatggtttct
      481 gcatcgatcc agaagatggc gctatattcc agggcgtgcc gataggcata ctccagcgcg
      541 atttgcgttt ttccgatccc tcccagccca cgtaaggcat atgattgggt gagagcgacc
      601 acctgctcca catgcagttg cgtatgcagt gcctccagaa tctcctcacg cccggtgaag
      661 aagggattgc gagctagagg gaccagccag tggggagaga gcgcggtcaa gctggcctcc
      721 agcagttggc gtgtctcatg ctcatccaga gccaggcgcc tggccagttc cagaaccagc
      781 ccgcgggtgt cgggcaaata ggtgcccagt tcccatgatg agatggtatt cgcatgcacc
      841 ccgagttgct gcgccaactg tttttgcgtc agtcgtttgc gcttccgacg ggtctttagt
      901 agctctccaa aactggatgg aaatgtctgc tggtcttcca tagatcctcc cgctctctcc
      961 gataagatga tcattgctgg gaaaagcggg actactccgc tcatccttct cttgcgatgt
     1021 aggccaatga agaactgagg gtggaacgct acgcggcacg ttttcacacg ctttcattga
     1081 cctagcataa ctatcgttgc atgaatgaag gtgttgccgg tcatttactg gatatacggc
     1141 tttgaatggt aaaaattcac agccagactg tgaatgcggc atgctagact tcctctagaa
     1201 agccgacaaa gaggtatttc gtcaccaggg agtgagcctc tgtcctggtg gaagatcaga
     1261 agatggctgt catctctctg atggttccgc atgagcgggt aaccaaatgc ataagttttt
     1321 ttatcctttt aggaggagac aatgctcaag caagccagaa aatcgcgttt tctgatgatg
     1381 tccttttccc ttttggcgac attcatcctg attctcagcg cctgtggagg aggggcgacg
     1441 acgtccacaa ctacatcgac cggtgcgccg gtgaagggcg gcacctggat tgatgatctc
     1501 tatgaagagc cggactcact gcttcccaat atgtcgagcg agacctttgc gaccatagtc
     1561 atggatggtc tatacgcgcc gctcatctat ggcacccctc aaggccagtt gatgcccggt
     1621 attgctacct cggtccccac agtccaaaat ggcggcgtga gcgctgatct caagactgtg
     1681 accttcaaca tgcgcccagg cctggtgtgg tccgatggca agccactgga cgccagggat
     1741 gtggattata gctggaagct gtgggcaaat cccaaggctg ccgcctataa caccgtgttc
     1801 gcccaggata tcgcgtcagc ggatattacc aacaacaacc tgacgattac cttccacctg
     1861 aagaatccgt ttgtgtcgtt cgtctcgcag tgggcggatg ggggactcgg tgccccgctg
     1921 ccggcacaca tcttttcgag catggaccca gccgccgtcc tccggtctcc gcaaaacctg
     1981 aatccgacgg ccacaagtgg cccgttcatg atgagcgaga gcaagcccgg cgaccactac
     2041 actattgtgc gcaacccgaa gtactacctg gcctcccagg gcctgccgta cctggatagt
     2101 gttgtgttcc gcattgtggc cgaccaaaac actatcttga aggatttgca ggcaggcagc
     2161 atcgattcgg cctggtttct ggatgtgagc aaggcggtgg cgtaccagtc gctgagcaac
     2221 taccaaatta tcggcgatgg cggcggtacg ggcacgaatg cgtatagctg ggaggcgatc
     2281 tggatcaact tcaacaacaa gatcctgggc ggcaatcccg aagtccggca ggcgatggcg
     2341 atggcggtag accagcagtc cctgatccag gtcgcccgtc gtggcttcgc tggcatccgc
     2401 tgccaggatc atccctccct tcaggtgccg ggctatacca ccagtctggc ctgcccgaag
     2461 tttgatctca cggcggccaa tgcgctgctg gatcagcatg gttgggtcaa aggctccgat
     2521 ggcgtgcgca gcaggaatgg gcagcggctg gagttccagt attcgactac cgccggcaac
     2581 ttatggcgca gtgacgacca gttgctcaac caggctaact ttatgaagat cggcatcaag
     2641 gtcgacatca tgaactaccc cgccagtacg ttcttcgggt cggtcctgcc tgcaggcaag
     2701 ccagggcagt atgacattgc cgagtttgca gaatccggag ccttcgaccc ggacaattcc
     2761 atacttttaa catgcgctga aaaagggtta gggaactttg ccttctactg cgcctcacag
     2821 atggaaaccc tgcaaaagca gcagttgtca accggtgata atactgcgcg ccagacggct
     2881 ttcgatggca tcaaccagct tgaggttacg gacaccccgt tcatcgtcga gtttagcgca
     2941 cccgacctgg cggtctacaa aaagggcacc cacaactacc tgccctcggc agtgggtgtc
     3001 ggctcaactg aaaacatctg gttgtggtgg tgtgacaatg gcacctgccc cgcctgagtt
     3061 gagcagcgct tgcgcccggg gttcgtagct gcgggcagat actgctcctg gtggctatct
     3121 ctcgacgata gccaccagag gagttctagt atgctgcatg ctaaatagtc acatcgttgt
     3181 aaaaatgtag cgactaagtc aatctaagaa ttccctagtg catgctcgtt ggaggcgcgt
     3241 atgggaaggt atattatcag gcgtattata caagcgattc cgttattgat cttgttgtca
     3301 gttgccatgt tcggcctcat ccacctgctg cccgggggag ccgatgcggt cttgttcaac
     3361 ccgcgcctga ccgcacaggc ccgtgccggg ctgcgcgcgc gcatggggct ggatgacccg
     3421 ttctatattc agtatttcaa gtggatcagc agcgcgttgg tgggcaactt tggctactcg
     3481 ttcaatacca atgaatccgt ctcgtcgatc ctggccaggc gctttcccgc gacgctcgag
     3541 ttgttcgtca cggcctttgt cgtggcctta acgcttgcca tcctgatcgg cgtgatctcg
     3601 gcggtgcgcc agggcaccct gaccgattac tcgctgacca tcctggccta cattggtatc
     3661 tcgatgccgg ccttcctgct gggactgttc ctgcaggaca tctttggcgt ctggctaaaa
     3721 tggctgccga cctcgggcac gggcagctac ggcgtcgcgt tctctctata tcagagcctg
     3781 gtggatcacc tggagcactt catcttaccg gtgacggcgc tatcgatcct gtttatcgcc
     3841 ggctggagtc gctacctgcg ctccagtatg atcgaggtgg tcaagcaaga ctatatgcgc
     3901 accgcgcgcg cgaagggggt cgattcgcga acgctgctct tgcgccatgc cttgcgcaac
     3961 gcggtgattc ccatgattac gattgtcgcg cttgatttcg gtgccatcgc cgggggggcg
     4021 acgattaccg agggggtgtt tgcctggccg ggcatggggt tgttgtttat tgacagcctg
     4081 gaccggcggg actacccggt actgatcgcc atgttgatgc tcagcgcgtt ctttgtgatt
     4141 gccttcaacc tgatcgcaga tatcctctac gcggtcctgg atccgcggat tcgctactcg
     4201 tgagcggtgg aggagatctg tttctgagaa ctggagtcaa ctgtggacat atccgaagat
     4261 ctgaatgcga cgagtacgac cgtcgaggag gtcgatccgt ttttagaact ggccccggcc
     4321 ctggtgcaga agcgacgcag tcaagggcgc attatctttg atcgctttat tcgcaacaaa
     4381 gcggccatcg gtggggccat cttcctgatc ctgctcttcg tcttctgttc cctggggccg
     4441 ttgttctggc aggtcgaccc caaacagcag aatattctga gtatatcgga gacgttagct
     4501 ccgccctcgc tggcgcatcc gttggggacc gacgatgtgg gccgtgatac gcttgcacga
     4561 gtgatggcag gaggccgggt ttcgctgtcg gtgggtttga cctcgatgct gatggccatt
     4621 ctctttggga tcggggtggg agccattgcg ggctactacg gtggggtgat ggacaatgtg
     4681 ctgatgcgag cgaccgacgt ggtgctggcc atccccctgt acctgctact gttcgtgctg
     4741 tcggcgtcgt ttaccgatgg cacccccaaa agtgtggtga tcttgatcgc gttctttggc
     4801 tggacctatg cggcgcgctt agctcgaggg gagtt
//
LOCUS       DASZAK010000067         7549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_108322, whole genome shotgun sequence.
ACCESSION   DASZAK010000067 DASZAK010000000
VERSION     DASZAK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108322"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VGT44_01070"
     CDS             complement(<1..358)
                     /locus_tag="VGT44_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579415.1"
                     /translation="MSTEQLPQANLQEERRLEAALRESEMLREITALLASSLDIEHIL
                     YVLVKRATQVCEVERCSVWLLDETRSLLRPKTYHLDTKPLDHRMIDAADRIWYRNPLA
                     MDDATIHQLFSEHGILY"
     gene            690..980
                     /locus_tag="VGT44_01075"
     CDS             690..980
                     /locus_tag="VGT44_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV2579416.1"
                     /translation="MGTRPPPKQHSIPSLLWEIIRLSARMVWGSRANPVRPVLERQQA
                     LAVLGLPPTATPQQIKRRYRELAKKHHPDRGGNSREMQRVIAAYQFLIKESS"
     gene            complement(953..1915)
                     /locus_tag="VGT44_01080"
     CDS             complement(953..1915)
                     /locus_tag="VGT44_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579417.1"
                     /translation="MEVILLYLHLAAALRELGQYEPANSQYVLARSLMDSSTPLRYIA
                     EAHWGTSLVLYEQAYKTETDQPQVTSESGQIPSSAEPAEISESFRTLMEEARTHAENA
                     RAMYQSANELLRASLLNCHIALIEQALNQFDSAQRRLQKILDEWLPTLSETPSPSTAP
                     LNGLATIQFEKRKSYSAKERANIVSATACYLASVERKTNRREAALAHIDLALDIGEHT
                     YIVRRADAYMTKGQILADTNDPGAIDAYKQALEELKGTDRLGAMIRVHKMLGAYLIRI
                     GSKEEGEAELDKALQLANVPTQFSATATDEYSMVNGQEDSLIKN"
     gene            1919..2344
                     /locus_tag="VGT44_01085"
     CDS             1919..2344
                     /locus_tag="VGT44_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579418.1"
                     /translation="MILRNRARHERDCCNEIGQRPGKLPDGKVALAAQREKTPAQVGR
                     DFIKRQRRDLFQGLLGSIRLEVLRGQDTLVFDMLEFVRLRFLIAPLSGQQVQIVNREI
                     EALSQVFQAFCRGFNFISLYLRDEAGTVFFTGNLGKRKM"
     gene            2885..4840
                     /locus_tag="VGT44_01090"
     CDS             2885..4840
                     /locus_tag="VGT44_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEV2579419.1"
                     /translation="MGKAKKQADYSAADIQVLEGIAAIRHRPGMYIGSTSSSGLLHLL
                     WEALDNAVDEAVAGFGKNIWVTLDREGWVTVLDEARGMPFDAMLYQGEYLPAATVILT
                     VPHSGGKFEEGVYKTAGGLHGVGSTIINALSEKLELTIWKDGQEFRQTFSRGTALPHS
                     ITPCDPKKHGTRLHWLYDSSIFDADAYYSLEAVESRLKASAYLNGGVTFHLDAWDDAE
                     EQQIKRVYYSKDGLPDYVRDLATSTSAPLFKQVLGIVKEKDGVQVEVALQPTAGYKIS
                     MYSYANAVRTRDGGVHETGFKAALTKVINDYALKWGVIKSREKDGFKPEVIQQGLNVA
                     ISVKLVNPQFQGQTKDRLNNAPVEGIVRSIVDEGLKEWFERNQAPGKDWLKKILQMQK
                     ARNEAQLVEELARAGNKRNGELIDTTLSKKFLRCNSSDPARSEIFIVEGDSAGGSAGQ
                     GRFSDYQAVLKLKGKPLNVAKADLKRIVENEEIRTIINVLGTGTRDSFDIGRLKFNRV
                     IIATDADVDGLHIQCLLLTLFHQEFSDLIERGHVYIACPPLYSVKYRGKVIWLLDDEA
                     RTQFIKSHVEAESSEFKRFKGLGEMNARELRETMFDPAHRTLKRVTMEDGVLAARLVG
                     QLMEDENAEARRTFLAEHARKIRELDV"
     gene            4846..7332
                     /locus_tag="VGT44_01095"
     CDS             4846..7332
                     /locus_tag="VGT44_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV2579420.1"
                     /translation="MARVSENNTPAGVIREEDFSVILSQAFATYGLSVVTDRALPDAR
                     DGMKPVQRRILAGMRDARFLSNRTTVKSAEVVGLILGNYHPHGDVSVYDAAVRLAQPF
                     TLRYPLIEGQGNMGSEDGDAPAAYRYTEMRLMPLAEALMADMEKETVPLHPSYKQDPK
                     VQEPDYLPGRVPPVVNPSSGIAVGLSTNIPPHNFTEVMRACIALLDKPEMPIEQLMSY
                     IPGPDFCQGGRVMGIDGIKDYMTTGKGRIIVRAEVRLEETPRSRLLVVTQIPPIGRDK
                     VKASIVKAINARKLEGLMPDMRDESDTEKGTRIVLELRKDADAAQVLSQLFSETDLQI
                     AQSFQMVFLFGESMQPARHPKQVGMIELLNYWNMHQVDVLTRRANFDLRKARERLHVV
                     EGLMIGAANAEKIVRIFQQAQDRSEARKKIETTYKLTSIQSEVIASMTLAQVTRLDAS
                     KYAKEREELQTLIAELEHLLADRGALIALLKKEMQQLIKQFGDERRTIIETDGQAHLP
                     VTEVASLHEREPLTIAYTRTGNIKALAADTYTPRGKNGNAAYMPVKGDEQLRQVVTAT
                     SQDYVLCVCTSGRVFQIAAHRIPEGTRSAKGEPLRKLLELAQGEEVIALLPLEAYDED
                     RYLVTFTRLGKVKKSPLSDYKTADVDGLQDMKLAEGDAVVAALISRGQGEYFITSDNA
                     QTLRFSDDALRAQGRVGQGVAAMSLAPHTAIVSAAYLDGEQENTASDPLSLFVVTASG
                     LGKKVPLSQYPLKGRATAGVITTELVGKDKVLSATLINEHAHILLISNGSGGEQVNAI
                     KASEIKMFPRARKGVPLVSGHLMSIVELTR"
BASE COUNT         1789 a         1969 c         2187 g         1604 t
ORIGIN      
        1 aataaaggat accatgctcg gaaaagagtt ggtgaatggt tgcgtcatcc atcgctaacg
       61 ggttgcgata ccagatacgg tctgctgcat caatcatgcg atggtcaaga ggctttgtat
      121 caagatggta ggttttagga cgcaggagac ttcgcgtttc atcaagcagc cagacagagc
      181 agcgttccac ttcacatacc tgtgtcgctc gtttgaccag tacatacagg atgtgctcga
      241 tgtctaacga agatgcaagc agcgcagtga tctcgcgcag catctcgctt tcacgcaggg
      301 ctgcctctaa gcgccgctct tcttgtagat tggcttgcgg tagttgttcg gttgacatac
      361 cttcgtcctt aaatgtgtct ggctcagtga atagacgttg ccccctctca aatatgatgt
      421 ttggtggctt caacaatcaa gtgtatcagg aggaggggaa attcaagtat gctgtcaatt
      481 acatatatag aagcaatatt aaggaaatta acgctcgcgc tcctgtgtga gatcgcaagg
      541 tagatgctgc aagtaaagct cttgcgaaga gtgtagcaga tgagcataag ttacgtcaag
      601 caagagggac ttttcgctcg ccgcgtgata gaatagtgac agcactgcgc tttgtggcta
      661 tgcgttatct ggcaaatgag cggacttata tgggtacaag gccaccgccg aaacaacaca
      721 gtataccatc attactctgg gagatcattc gcctgagcgc gcgtatggtt tggggtagcc
      781 gcgctaatcc ggtgcggccg gtgttggaac gacagcaagc gctggcggta ttgggtttgc
      841 cgccaacggc cacaccccag cagatcaagc gacgataccg ggaacttgcc aagaagcatc
      901 atcccgacag gggaggcaat tcacgtgaaa tgcagcgagt gatcgccgca tatcagtttt
      961 tgatcaaaga atcttcttga ccattaacca tggagtattc gtcggtggct gtcgcgctaa
     1021 attgggttgg aacattggcg agttgaaggg ctttgtccag ctctgcttcg ccttcttcct
     1081 tgctgccgat gcgaatcaga tacgctccaa gcatcttatg gacacgaatc atagcgccca
     1141 gccggtcggt acctttcaac tcctcgagtg cctgcttata tgcatcaatc gctccagggt
     1201 cgttggtgtc ggccagaatc tgtcctttgg tcatgtacgc atccgcgcgt ctcacaatat
     1261 aagtgtgttc cccaatatcc agcgccaggt cgatatgggc cagcgctgct tcgcgccggt
     1321 tcgtttttcg ctcaacactg gcgagatagc aggcagtagc tgaaacgatg ttggcgcgct
     1381 ctttcgcaga atagcttttc ctcttctcaa actgaatagt tgccaatcca ttcaatggtg
     1441 ccgtggaagg cgaaggggtt tcggagagcg tggggagcca ctcgtcgagg attttctgca
     1501 gccttctctg ggcggagtcg aactggttca gcgcctgctc gatcaaggca atatggcaat
     1561 taagcagcga ggctcgcaat agttcgttgg cggattgata catggcacgc gcattttccg
     1621 cgtgcgttcg cgcctcttcc atgagcgtac ggaaagattc gctgatttct gctggctccg
     1681 ccgatgatgg tatctggccg ctctcactgg tcacctgagg ttgatcggtc tctgttttgt
     1741 aggcttgttc atacaaaaca agtgaagttc cccaatgggc ttcagcaata tagcgaaggg
     1801 gagtggatga gtccatgaga gaacgcgcta gcacgtactg gctattcgcc ggttcgtatt
     1861 gtcccaactc cctcaacgcc gcggccagat gcaggtaaag taggatcact tccatggttt
     1921 gatcctgcgg aaccgggcga ggcatgaacg cgactgctgc aatgaaatcg gtcagcgccc
     1981 aggtaaattg ccggatggca aagtagcact ggccgcgcag cgcgagaaga cgccagcgca
     2041 ggtaggtcgg gatttcatta aacgccagcg tagagatttg ttccagggcc ttcttgggtc
     2101 gattagactc gaggtattgc gtggtcagga cacgctggtc ttcgatatgc tggaatttgt
     2161 tcgactccgc ttcctcatcg cgccgttgtc tggccagcaa gttcaaatcg tcaatcgaga
     2221 gattgaggcg ctgagccagg tatttcaagc tttctgtcga gggttcaatt ttatttcgct
     2281 ctatttgaga gatgaggctg gtactgtatt tttcaccggc aacctgggca agcgaaagat
     2341 gtaggcgttg acgcgccgct cgaattcgtc ctcccagggt atctgctgga acacttttta
     2401 atggacgtgg cataagtcgg gcactttctc agtaggggtt actgcatagg agttacatgg
     2461 ctacgcaggt tacatataca aattgttaac atggaacgac tggtttttcc tatataatcc
     2521 attatacgca gggagatact atagaattac gttactttga catcgaactt cactgattac
     2581 agtatgtatt gtggccatat tgtatcaaaa aggtcatgca atggcaagtg gtaagacaaa
     2641 agtttagaac aaactaccaa aaaactattg acagaaagaa tagaacactg tatgctgatg
     2701 gtagctacaa agccacgtga agagaggaag aagctatatg gctcgttcag ccaggttatc
     2761 ccccgcgacg caagacgaag tggaaggtca gttatcactg ttttccgcga agcgcgcgga
     2821 agacctaaaa ataacagaca acaaagagca gcctgagatg ttactcagaa aagagataaa
     2881 atctgtggga aaggcgaaga agcaggccga ctattcagcc gccgatatcc aggtgcttga
     2941 aggcatcgcc gcgatccggc atcgcccagg tatgtacatc ggctcaacat cctcgtccgg
     3001 tttgctgcac ctgctctggg aggcgctgga taatgctgtg gatgaggccg tggcaggatt
     3061 tggcaaaaat atctgggtca ctttggaccg agaaggctgg gtgacggtcc tggatgaggc
     3121 gcgtggtatg ccgttcgatg ccatgctcta ccagggggaa tacctgcccg ccgcgaccgt
     3181 catcttgacc gtacctcatt ctggtggaaa atttgaggaa ggtgtctata aaacggctgg
     3241 cgggctgcat ggcgttggtt caaccatcat taatgcgctt tccgaaaagc tggaattgac
     3301 aatctggaag gatggccagg aatttcgcca gacgttcagc cgggggacag ccctgccgca
     3361 ctcgatcact ccctgcgatc ccaaaaaaca tggcactcga ctacactggc tgtatgatag
     3421 cagtatcttt gatgccgatg cctactattc acttgaagct gtcgagagcc gcctgaaggc
     3481 atccgcctat ctcaatggcg gcgtgacctt ccacctggat gcctgggatg acgcggaaga
     3541 gcagcagatc aagcgcgtct actattcaaa ggatgggttg cccgattatg tgcgcgacct
     3601 ggccaccagc accagcgcgc cactcttcaa acaggtgctt ggcatcgtaa aggaaaagga
     3661 tggtgtgcag gtcgaggtag cgctgcagcc gacggctggc tacaagattt ccatgtatag
     3721 ctatgctaac gccgtgcgga cgcgcgacgg tggtgtgcat gagacgggct tcaaggccgc
     3781 gctgacgaag gtcatcaacg attatgctct caagtggggc gtcatcaaga gccgtgagaa
     3841 ggatggtttc aagccggaag taatccagca gggccttaac gtggccattt ccgtcaagct
     3901 ggtcaacccg cagtttcaag gacaaaccaa agatcgcctg aacaacgccc cggtggaagg
     3961 catcgtgcgt tcgattgtcg atgaaggttt gaaagagtgg tttgagcgca accaggcccc
     4021 tggcaaagac tggctcaaga agattctcca gatgcaaaag gccagaaacg aggcgcaact
     4081 tgtcgaggaa ctggcgcggg ccggcaataa acgcaatggt gagctgatcg atacgacgct
     4141 atcgaagaaa ttcctgcgtt gtaacagcag cgacccggcg cgttctgaaa ttttcatcgt
     4201 ggagggcgac tcggctggcg gcagcgcagg gcaggggcgt ttctcagact accaggccgt
     4261 gctgaaactg aaaggcaagc cgctcaatgt ggcgaaggcc gacttgaaac gcatcgtgga
     4321 gaatgaagag atccgcacca ttatcaacgt gcttggtacc ggtacgcgcg attccttcga
     4381 catcgggcgg ctcaaattca atcgcgtcat tatcgcgacc gatgccgacg tggatggcct
     4441 gcatatccag tgcctgctgc tgacgctttt ccaccaggag ttcagcgacc tgatcgagcg
     4501 tggtcatgtc tacatcgcct gcccgccgct ctactcggtc aagtaccggg gcaaggtgat
     4561 ctggctgctg gacgacgagg cgcggacgca gttcatcaaa tcgcacgttg aggctgaaag
     4621 ttcagagttc aagcgcttca aaggtctggg cgagatgaac gccagagagc tgcgcgaaac
     4681 gatgttcgat cctgcgcatc gcacactcaa acgtgtcacg atggaagatg gcgtgctggc
     4741 ggcgcggctg gtcggtcaac tgatggaaga tgaaaacgca gaggcgcgca ggaccttcct
     4801 ggcggaacac gcgcgcaaaa tccgagaact ggatgtataa aaaacatggc acgagtaagt
     4861 gaaaataata cacctgcggg cgtcattcgc gaagaggatt tctccgtcat tctttctcag
     4921 gcgtttgcca cctatggcct gtcggtcgtc acggaccgcg ccctgccgga tgctcgcgat
     4981 ggcatgaaac cggtgcagcg gcgcatcctg gcaggcatgc gtgacgcgcg cttcctctcc
     5041 aaccgcacga cggtcaaaag cgcggaggtg gttggactga tcctcggtaa ctatcatcca
     5101 catggcgacg tatccgtcta cgatgccgcc gtgcggctgg cccaaccgtt cacactgcgt
     5161 tatccgctga tcgaaggcca gggcaacatg ggcagcgagg atggcgatgc gcctgctgcc
     5221 taccgctata ccgagatgcg gcttatgcca ttggctgagg cgctgatggc cgatatggaa
     5281 aaagaaacgg tgccgctcca tcccagctat aagcaggacc caaaggtgca ggagccggat
     5341 tacctgccgg gacgcgtccc gccggtcgtc aacccatcca gcggcatcgc ggtcggcctc
     5401 tcaaccaata tcccgccaca taacttcacc gaagtgatgc gcgcctgcat agccctgctc
     5461 gataagccgg aaatgcccat tgagcagttg atgagctata taccagggcc ggatttctgc
     5521 cagggtgggc gcgtcatggg catcgacggc atcaaggatt acatgacgac gggtaagggg
     5581 cgtattattg tgcgcgccga ggtgcgcctg gaagagacgc cgcgcagccg cctgctggtc
     5641 gtgacgcaga ttccgccaat tggccgcgac aaggtcaaag cctcgatagt caaggccatc
     5701 aacgcgcgta agctggaggg gttgatgccc gacatgcgcg acgagagcga tacggagaag
     5761 gggacgcgca tcgtgctgga actgcgcaag gatgccgatg ccgcgcaggt gttgtcgcag
     5821 ttgttcagcg aaaccgactt gcaaatcgcc caatccttcc agatggtctt tctctttggc
     5881 gaatcgatgc agcccgcgcg acatccgaag caagtgggca tgatcgaact gctcaactac
     5941 tggaatatgc accaggtcga tgtgctgacg cgccgcgcga acttcgacct gcgcaaggcc
     6001 agggagcgat tgcacgtcgt cgagggcttg atgatcggag cggccaacgc tgagaagatc
     6061 gtcagaatct tccagcaggc gcaggatcgt tccgaggcgc gcaagaagat cgaaacgacc
     6121 tacaagctga cgtccatcca gtccgaggtg atcgcctcga tgacgctggc ccaggtgacg
     6181 cgcctggatg cgagcaagta cgcgaaggaa cgcgaagaac tacagacgct gatcgccgag
     6241 ctggaacacc tgctggcgga tcgcggcgcg ctgatcgccc tgctcaagaa agaaatgcag
     6301 cagctcatca agcaatttgg cgatgagcgg cgcacgatta tcgagaccga tggtcaggcg
     6361 catttgcctg tgaccgaggt agcgagcctg cacgaacgcg aaccgctcac gattgcctac
     6421 acgcgcacgg ggaacatcaa ggccctggcg gcagatacct atacgcccag gggtaagaat
     6481 ggcaacgccg cctatatgcc ggtcaagggt gacgagcagt tgcgccaggt ggtgaccgcg
     6541 acctcgcagg attacgtgct atgcgtctgc acgagcgggc gcgtcttcca gatcgccgcg
     6601 caccgcatcc cggaggggac gcgctcggca aagggcgagc cgctgcgcaa gttacttgag
     6661 ctggcccagg gcgaagaggt catcgcgctg ctgcctctag aggcatatga tgaagaccgt
     6721 tatcttgtga ccttcaccag gctgggcaag gtcaaaaaat ctccattgag tgattacaaa
     6781 actgctgatg tcgatggcct gcaagatatg aagctggccg aaggggatgc tgtcgtggcc
     6841 gcgctcatct cgcgcggcca gggagaatac tttataacca gcgacaacgc gcaaaccctg
     6901 cgctttagcg acgatgctct gcgcgcgcag ggccgcgtcg gtcagggcgt cgcggcgatg
     6961 agcctggccc cccataccgc catcgttagc gccgcctacc tggatggcga acaggagaac
     7021 acggccagcg atcccttaag cctctttgtc gtcacggcga gcggcctggg caaaaaagtg
     7081 ccgctcagtc agtatcccct gaaaggccgc gccaccgccg gtgttatcac aaccgaactg
     7141 gtcggtaaag acaaagtgct gtcggccacg ctcatcaacg aacacgccca catcctgctc
     7201 atctcgaacg gctccggagg cgagcaagtc aacgccatca aagcctcgga aattaaaatg
     7261 tttccacgcg ccaggaaggg tgttccactg gtgagtggtc atcttatgag cattgtagaa
     7321 ttgaccaggt aaggaattgt cgtagggacg ggacgcgatg catcccgtcc ctacgacaat
     7381 aggtgaatca atgccactcg tctcaattca taacaaggat acaatagagc aatacctgcg
     7441 caagaacacg cccattcatc tttacgaact tggtgacctg gacgaattct actggccatc
     7501 cacgacgtgg tacgcgctgg aagaagctgg gcgagttcgg gcgcttgcg
//
LOCUS       DASZAK010000068         1895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_427697, whole genome shotgun sequence.
ACCESSION   DASZAK010000068 DASZAK010000000
VERSION     DASZAK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427697"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1895)
                     /locus_tag="VGT44_01100"
     CDS             complement(<1..>1895)
                     /locus_tag="VGT44_01100"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2579421.1"
                     /translation="ITEQSHPTIHTPALKQFAIGKLKQEQLLAYPAGIEHAQEIEMVN
                     ARVEEIVSRGKYVRLSDGRDFGYDALLLATGSKANGLPAGMPGSSFDGVLTLHRLSDY
                     LDLRRRLELREVKEAVVIGGGTHAFENVMGLLYLGVRVHWLIRGSTFLPNTLDSIASE
                     LALEHARHAGAKVSLDTEVVGIVGRVGSVAGVVTNHHQTLTCQLALVCTGASPLTTLA
                     ERCDVPLDRQRGICVDDHLRSSVRDIYAAGDVAALKNARTGVFQPRAHWHDAVLQGRV
                     AAAVMTGCTDLVSPLGIAWHATQMGGLSMLTVGDPLRESEGTEMLTNSRKGGYRRLVT
                     RGDRLVGYLSLGPTQPDSLAVKRLIEEGRSIRGIEKELLSGEFDARQYFIQQQTHVVR
                     KAATTRKLALPGMSPQPAYVAQNTEPLPVLSDHQLERSGELPLLQPPTRPAPVINSLT
                     SLMSEQPDAAGWIIPELLPQGLVLLAGNEEFSNTGLDLVLGLGVASGGSVFENFEVEQ
                     GNVLYLAFNENERGLQDRLRKLMPSRRMPPEQFEYATGWPKMDDGGLDSLEEWLIARA
                     HPRLVILDAWPAQRDKWFSYEGEDDILDGLRSLANDYHVCILARYNGELRVESNAPEC
                     ADALLQL"
BASE COUNT          409 a          608 c          506 g          372 t
ORIGIN      
        1 ttcaactgca agagagcgtc agcgcactca ggagcgtttg actccaccct caactcgcca
       61 ttgtagcgcg ccaggataca gacatgatag tcgttggcaa gcgatctcaa gccatccagg
      121 atatcatcct ccccctcata gctgaaccac ttgtcacgct gtgcaggcca ggcatcgagg
      181 ataaccaggc gtggatgcgc gcgcgcaata agccattctt ccaggctgtc aaggccgcca
      241 tcgtccatct tcggccagcc tgtggcatac tcaaattgct ctggcggcat cctgcgcgac
      301 ggcatcaact ttctcagccg gtcttgcagg ccgcgctcgt tttcgttgaa ggcaagatat
      361 aagacattgc cctgctcaac ctcgaagttc tcgaaaacgc tgcctccact ggctacaccc
      421 agcccgagca ccaggtcaag gccagtgttg ctgaactcct cattgccggc cagcagcacc
      481 agcccttgcg ggagtagctc aggaataatc catcccgccg catcaggctg ctcactcatc
      541 agtgaggtga gcgaattaat tacaggagct ggccgcgtgg ggggttggag tagaggaagc
      601 tcgccgcttc gctccagttg atggtcggag agaacgggca gcggctcagt attctgcgcc
      661 acatacgccg gctgaggcga catgccggga agcgccagtt tgcgggtcgt tgctgccttc
      721 cggacaacgt gtgtctgttg ttggataaaa tactgccgtg catcaaattc gcccgacagc
      781 aactccttct cgatgccgcg gatagagcgt ccttcttcga tcagtcgctt caccgccagg
      841 ctatctggct gggtcggccc cagcgagaga taccccacca atcgatcgcc gcgcgtcacc
      901 aggcggcgat atcctccctt tcgactattc gtgagcattt ccgtgccttc agactcgcgt
      961 aacggatcgc ccaccgtcag catcgacagg ccgcccatct gtgtggcatg ccacgcaatg
     1021 ccaagcggcg agacaaggtc cgtacagccg gtcatcaccg ccgcggcaac gcgcccttgc
     1081 aaaacagcat cgtgccaatg cgcgcgcggt tggaacacgc cggtgcgcgc attcttcagc
     1141 gcggcaacat cgcccgcggc gtagatatcg cgcacgctgg aacgcaagtg atcgtccacg
     1201 caaataccgc gctggcgatc aagcggaaca tcgcagcgct cagccagcgt cgtcagcggg
     1261 gacgcgcctg tgcagaccag cgctaactgg caggtcagtg tctggtggtg gttcgtgaca
     1321 actcccgcca cgctgccgac tctgccgacg atacccacca cctccgtatc cagagaaacc
     1381 ttcgcgcccg cgtgccgggc atgttccaga gctagctcag atgcgattga atcaagcgta
     1441 ttcggtagaa aagtcgagcc gcgaatgagc cagtgaacgc gtacacccag gtacaacaat
     1501 cccatcacgt tctcaaaggc atgcgtgccg ccgccaatca caaccgcctc cttcacttct
     1561 ctcagctcta atctgcggcg caaatcaaga taatcactca ggcggtgcag cgtcagaacc
     1621 ccatcaaaag agctacccgg cataccggca ggcagaccgt tggccttact gcccgtcgcg
     1681 agcagtagag catcataacc aaagtctcga ccatcgctca agcggacgta tttccccctg
     1741 gaaacaatct cctccacccg cgcattgacc atctcgattt cctgagcatg ttcgataccc
     1801 gcggggtagg ccagcaattg ctcctgcttc agcttgccaa tggcaaattg tttgagcgcg
     1861 ggcgtatgaa tggtaggatg gctctgctcg gtaat
//
LOCUS       DASZAK010000069         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_498612, whole genome shotgun sequence.
ACCESSION   DASZAK010000069 DASZAK010000000
VERSION     DASZAK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498612"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VGT44_01105"
     CDS             complement(<1..1434)
                     /locus_tag="VGT44_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEV2579422.1"
                     /translation="MTAQSDVFGARASLRGAYGNVKYYALDALARRGISGLERLPYTV
                     KIILENALRHAGGELVTEEEVLSLARWTPGQSSQSDYEYPFMPARVLLQDLTGVPAVA
                     DLAAMRSAVARMGGDSHKINPLVPADLVIDHSVQVDMFGSTLALDRNVEREYERNSER
                     YGLLRWGQQAFSNFRVVPPGTGICHQVNLEYLASVVMTREEDGETIAYPDTLVGTDSH
                     TTMINGLGVLGWGVGGIEAEAVLLGQPTYLLNPEVIGVRLSGALPEGSTATDLVLTVT
                     QMLRKRGVVGKFVEFTGSGLSFITVADRATISNMSPEFGATATLFPVDEETLRYLRTT
                     GRDAKLVELVEHYTKAQHLFRTDDVPEPRFDDLIELDLGTIEPSLAGPRRPQDRVPMQ
                     NLGKVFRDAYADRFVPPQINHATEDALLRLETESNDTGKDPVAMKEDSDKARSAEKRG
                     GDGHNGHLRDVVVTMGNAQAHMTDGSVA"
     gene            complement(1985..>2872)
                     /locus_tag="VGT44_01110"
     CDS             complement(1985..>2872)
                     /locus_tag="VGT44_01110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2579423.1"
                     /translation="VGPLLPVVDIYDDAVGEIAWLFFLVPLFAIYFLALVTTRLGIRT
                     DTLQHTLSDLSSARRRQHELSDYASLVTRVQEEERRRLARELHDDTAQALIALSLGLD
                     GLGRAMANRDVPDKDVQWLESLQNLADHTLDGVRRACRDLRPSVLDDLGLRAALEWLS
                     DSSSSRGVPCTFTCDGVPLVTSAEEEIAIFRIAQEALSNVWRHAQANQARLELLYKEQ
                     LIRLTVQDNGKGFLPLSERQAGSQVGFGMVGMRERAALMGATLLITSSPDNGCSVVLT
                     LPLTPGATDHENIHEISSF"
BASE COUNT          589 a          848 c          869 g          566 t
ORIGIN      
        1 ggcgaccgag ccatcggtca tgtgcgcctg cgcgttgccc atcgtcacaa ccacgtcgcg
       61 caggtggccg ttgtgtccat cgcctccgcg cttctctgcg ctcctggctt tatcgctatc
      121 ctccttcatg gcaaccgggt ctttgccggt atcgttgctc tccgtttcca ggcggaggag
      181 cgcgtcttct gtggcatgat tgatctgcgg cggtacaaaa cgatccgcat aggcgtcgcg
      241 aaagacctta cccaggtttt gcatggggac gcggtcctgc ggtcggcgcg gtcccgccag
      301 gcttggctct atagtgccga ggtcaagttc gatcaggtca tcgaagcgcg gttccggcac
      361 gtcgtcggtg cggaagagat gctgcgcctt cgtatagtgt tccaccagtt caaccagttt
      421 ggcatcgcga ccggtcgtgc gcaggtaacg cagggtctcc tcatcaaccg ggaagagcgt
      481 agcggtcgcg ccgaattcgg gcgacatgtt ggagatggtg gcacggtcgg ccactgtgat
      541 gaagctgagt ccgctgccgg tgaactcgac aaacttgccg acgacgccgc gtttgcgcag
      601 catctgcgtc acggtcagca ccaggtcggt cgcggtcgaa ccttcgggga gcgcgccgct
      661 cagccgcacg ccgatgacct ccgggttgag caggtaggtg ggctggccga gcaagacggc
      721 ctcggcctcg atgccgccga ctccccagcc aagtacgccc aggccgttaa tcatggtggt
      781 gtgtgagtcg gtgccgacca gcgtgtcggg atacgcgatg gtttcgccgt cctcttcgcg
      841 cgtcatcacg acggatgcca ggtattccag attgacctgg tggcagatgc cggtgccggg
      901 aggcacgacg cggaagttgc tgaatgcttg ctggccccag cgcagcagcc catagcgctc
      961 gctgttgcgt tcgtattcgc gctcgacgtt gcgatcgagg gccagcgtcg agccgaacat
     1021 atcgacctgc acggaatggt cgatcaccag gtcggcaggc acgagcggat tgatcttgtg
     1081 cgagtcaccg cccatgcgcg ccaccgccga acgcatggca gccaggtccg cgacagcagg
     1141 cacgccggtg aggtcttgca gcaggacgcg cgcgggcata aaggggtatt cgtaatccga
     1201 ctgggacgac tggcctggcg tccagcgcgc cagcgagaga acctcctcct ccgtcaccag
     1261 ctcgcctccg gcgtgacgca gcgcgttctc caaaataatc ttgacggtat agggcaggcg
     1321 ctccagccct gagatgccgc gccgggcgag cgcatcgagc gcgtagtact tcacgtttcc
     1381 atacgcgccg cgcaacgatg cgcgcgcgcc aaatacatct gattgagcag tcatgatttc
     1441 atcctctgaa gcgtttattg gagtcaatgt ccattgcatt atataaccta gggatttggg
     1501 gagtagggta tcaccagggc ggaagccata cgataatcat atgaactttt accaccgaac
     1561 tcgggaatgt catgctgagc gaagcgaagc atctggcagc ccatcgaccg agacccttcg
     1621 cttcgctcag ggtgacattc ctgaacctgg ttgggaaatt tcatgatcgg cgggacaatg
     1681 catgtcgagc cacgcgatac aaataatcag ggcgacaacc agcgcagccg actgtacagt
     1741 gtaccagcag cgagagagaa caccgcacgg agagacaaag atgcttcact cttctggaga
     1801 agatgacagt tttttacgtc aatagagcgt tctcgtgggt tggtcccggt tccgcgccac
     1861 gcttctccat caacctgtca tttccctggt acgcagcata gtcggcaggc gggttattcg
     1921 ccctgccgtc tatactatgc agaatctcgc gggaaattac atcagcataa atgcctaaaa
     1981 cgtctcaaaa tgaggaaatt tcatgaatat tttcgtgatc ggtcgcgccg ggcgtaagtg
     2041 gcagcgtgag tacgaccgag cagccgttat ccggcgagga ggtgattaag agggtagctc
     2101 ccatgagcgc ggctcgttcg cgcatcccaa ccatgccaaa gccaacctgc gaaccggctt
     2161 gccgttctga gagagggaga aagcctttgc cgttatcttg aacagtcagg cgaataagct
     2221 gctctttgta gagcaactcc aaccgggcct gatttgcctg ggcgtgtcgc cagacattcg
     2281 acagggcttc ttgcgcaatt cggaaaatag caatctcctc ctctgccgag gtcacgagcg
     2341 ggactccatc gcaggtgaag gtgcatggta cgccgcgcga cgaagagctg tcgctgagcc
     2401 attccagggc cgcgcgtaga cccaggtcgt cgagaaccga ggggcgcaag tcgcgacagg
     2461 cgcgtcgcac cccatccagg gtgtggtcgg ccaggttttg caggctttcc agccactgca
     2521 catctttgtc aggcacatca cggttcgcca tcgcccgacc caggccatcc agcccaagtg
     2581 aaagcgcgat aagtgcctgg gccgtatcgt cgtgtagctc gcgcgctagc cgacggcgct
     2641 cttcctcctg gacgcgcgtc accagcgagg cgtaatcact caactcatgc tgacgccgac
     2701 gcgcgctgct caaatccgag agcgtgtgct gcagggtatc ggtgcgtata cccaggcgcg
     2761 tcgtaacaag cgccaggaag tagatggcaa acaagggcac gagaaaaaag agccaggcga
     2821 tttcgccaac cgcatcatca tagatatcaa cgaccggcaa aagagggccg ac
//
LOCUS       DASZAK010000070         1567 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_72732, whole genome shotgun sequence.
ACCESSION   DASZAK010000070 DASZAK010000000
VERSION     DASZAK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72732"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VGT44_01115"
     CDS             <1..1163
                     /locus_tag="VGT44_01115"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2579424.1"
                     /translation="TQQTCDALLELLSSCGPIEPQEGRVCLTTSGTMGEPKGVVLSER
                     QVAWTADHIRTSHHLSTRDRGLTVLPFFHVNAPVVSLCSSLLAGSTVVIAQRFSRRNF
                     WSWIERYQVTWASIVPTIVAILLETERPAFLPGALRFMRTGSAPLPAAHLLAFEARFG
                     IPVIETYGLSEAASQVVANPLPPGVHKPGSAGRPVGVDLRICYPRTAEGGGLLRDVGP
                     GETGEICIAGPSVIGSYQDNTGQEAFQDGWFRTGDLGYLDEDGYLFIRGRLREVIKRG
                     GETIAPREVEEALLSHAAVREAAVVGRPDAIYGEQVVAYVAVKGAWSAEMARELQQHA
                     AQRLSPQKVPVDFTALDALPRSPTGKIERRLLRAREQARVAASEVENDVRAS"
     gene            complement(1177..>1567)
                     /locus_tag="VGT44_01120"
     CDS             complement(1177..>1567)
                     /locus_tag="VGT44_01120"
                     /inference="COORDINATES: protein motif:HMM:NF025397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579425.1"
                     /translation="LQAGSYVVMDAFHAQLVPGFPVALTVLGTVISRQGHRVVFDPGR
                     KVIGSELGLPRLKDVPSTTAGIAEEHLLVDVDPGYPLKVGDRVEVISGYGPTTVNLHD
                     VYYVVENDVVTDVWPIRARGAGLGPFC"
BASE COUNT          332 a          450 c          456 g          329 t
ORIGIN      
        1 tcacccagca aacgtgcgac gcgctgctgg aactgctatc ttcctgtggc cctatcgagc
       61 cccaggaagg acgggtctgc ctgacgacat ctggaacgat gggtgaaccg aagggtgtgg
      121 ttctcagcga acgccaggta gcgtggacgg ctgatcatat tcgcaccagt catcacctct
      181 ccacccggga tcgcggttta acagtcctgc cattcttcca tgtgaacgcg cctgttgtca
      241 gcctgtgttc gtctttgctg gccggcagca cggttgtcat tgcccagcgc ttcagtcgca
      301 ggaacttctg gtcatggatc gagcgctacc aggttacctg ggcgagtatt gtgcccacca
      361 tcgtcgccat attattggaa acggaaaggc ctgctttcct gccgggcgct ttgcgcttca
      421 tgcgtactgg ctcagctccg ctgcccgctg cacatctact ggcttttgaa gcacgctttg
      481 gtattcctgt catcgaaacc tacggtttat cagaagctgc aagccaggtc gttgctaatc
      541 cactaccgcc aggcgtccac aaaccgggat cggccggccg gcctgttggc gtggacctgc
      601 gtatctgcta ccccagaact gctgagggtg gggggttgct acgcgatgtc gggccaggag
      661 aaacaggcga aatctgtatt gccggaccca gcgtgattgg ctcgtaccag gacaataccg
      721 ggcaagaggc atttcaagat ggatggtttc ggactggtga ccttggatac ctggatgagg
      781 atggctacct gtttatcagg ggccgtttgc gcgaggtaat taagcgcggc ggtgaaacta
      841 ttgcgccgcg cgaagtcgag gaagcgctcc taagccacgc ggcggtgcgc gaggctgctg
      901 ttgttggtcg cccagatgct atctatggcg aacaggttgt ggcatacgtc gctgtaaagg
      961 gcgcctggag cgcggagatg gcacgagagc tgcagcagca cgcagcccag cgcttaagcc
     1021 cgcagaaagt accggtggac tttactgcgc tcgatgcgct gccaagaagt ccgaccggga
     1081 agatcgaacg gcgtctttta cgtgcgcgag aacaggcgcg tgtggcggca agtgaagtcg
     1141 agaatgatgt gcgagcgtct tagccgacct caaatactag caaaatggcc caagaccggc
     1201 gccacgtgcc cggataggcc atacatccgt aaccacatcg ttctctacga cgtagtaaac
     1261 atcgtgaagg ttgaccgttg tcggaccata gccggagata acctcaactc gatccccaac
     1321 tttcaatgga tagcctgggt ccacgtcaac gagcaaatgc tcctcggcga ttcccgctgt
     1381 ggtggacggc acatctttga gacgcggcaa tccaagctca ctaccgatta ccttccggcc
     1441 tggatcaaaa acaaccctgt gcccctgccg gctgattacc gttcccagta ccgtcagcgc
     1501 tacaggaaaa ccaggcacca gctgcgcatg gaaagcatcc atcaccacgt atgacccggc
     1561 ttgtagc
//
LOCUS       DASZAK010000071         2505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_250353, whole genome shotgun sequence.
ACCESSION   DASZAK010000071 DASZAK010000000
VERSION     DASZAK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2505
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250353"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGT44_01125"
     CDS             <1..366
                     /locus_tag="VGT44_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579426.1"
                     /translation="FLNPYQDARFTSCPQCGIKTRQRKLPLVIHVDPMYTLILGKTCR
                     YCVNCDLLIAHQDQLEDQLSMYFSTINPESIGNDYLVMGTLDRPEWRQGMQDPLSIQE
                     MIEHLHDFKEVITFQPSYV"
     gene            complement(377..2470)
                     /locus_tag="VGT44_01130"
     CDS             complement(377..2470)
                     /locus_tag="VGT44_01130"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEV2579427.1"
                     /translation="MTDKHNSDAKQRILLIALLILMLLAVVLVAARYWPFNQAGVVTD
                     TCAATLGAQPAVTVKTRTTPPLYLGLDAYRHLDKLSYLELGDRVEGASTADPDGSNAD
                     YTHVARVLPDGEHVLFDQLGPGIMTFMRMQENYGGPWHLSLDGHAITTVNSSDLGQTN
                     PGSSPASDFPYPLSLNPQISQGSSIVATSIPFQQSMQWASQHANGNFYALYRKLPYGA
                     ALQTWNGSEPVNDVVGLLRCAGGDIAPQAIAQQRGTLSFTANKQTPVVTLSGPAQIRA
                     LDFQVSPQEMVAFGNARLLIYWDGESQPSVDAPIKFLAGDGAGIYFPQNRSLVQGLMA
                     GISQNQSGAMDVHLYWPMPFSSQARIALLADAPLNGVQWNVGYEPFPDPPQWWGTFHA
                     TYSSVANPTFGQDMTFLDVQGSGKLVGTVINFTTPGGTLEGDPHIYLDDSLTPQIAAT
                     GTEEWGLGGNYWNGGQQVSLPLGGLPSSINNPPGTNIDGAALYRYLIADSIPFNRRLI
                     VRWEHGGVDQGLLPYRATMLWYGTPTQTALLSDSVSPAAFASRLAHRYTATGEQTYPL
                     TAAYEYVVNAPLLTATGTALTGSATFTMQLDPRNVGTFLRRTFDDCVGNQRANVYLDN
                     SFAGTWYDAGVSNGKGIDGHLRCWRDEDFPLPAPLTAGKSSVQVRIVFLPTTNPQNSA
                     WTAFRYSMYSFVVPT"
BASE COUNT          540 a          708 c          724 g          533 t
ORIGIN      
        1 ttcctcaatc cctaccagga tgcccgtttc acctcctgtc cgcaatgtgg gatcaagacc
       61 cggcagcgca aactgccgct tgtcatccat gtcgatccaa tgtacaccct cattctaggg
      121 aaaacctgcc gctactgcgt caattgcgac ctgttgattg cccatcaaga ccagcttgaa
      181 gaccaactgt ccatgtactt ttcaaccatc aaccctgaga gcattggcaa cgactacctg
      241 gtgatgggga cattggatag gcccgaatgg agacaaggga tgcaagaccc cttgtccatt
      301 caggagatga ttgaacatct ccacgatttt aaagaagtta ttacatttca gccgtcatat
      361 gtctagacaa taagagctac gttggtacga caaaactgta catcgaatag cgaaaagccg
      421 tccaggcgct gttctgtgga ttggttgtag gcaggaacac gatgcgcacc tgtaccgaag
      481 actttccggc ggtcagcggt gctggcagag gaaaatcttc atcgcgccag catcgcagat
      541 gtccatcaat gcctttcccg ttggatacac ccgcgtcata ccacgtaccc gcgaagctgt
      601 tgtcaagata gacgttggcg cgctggttgc cgacgcaatc atcgaaggtg cggcgcaaga
      661 atgttccgac gttgcgcgga tcgagctgca ttgtaaaggt cgcactcccc gtcagagcgg
      721 ttccagtcgc ggtaagcaat ggggcgttga cgacgtattc ataggcggcg gtaagggggt
      781 aggtctgctc gcccgttgcc gtgtaacgat gcgccaggcg gctggcgaac gctgccggtg
      841 acacgctatc gctgagcagg gcggtttgcg tcggtgtgcc ataccacagc atggtagcgc
      901 gatagggaag gaggccctgg tctactccgc catgttccca gcgcacaatc agacggcgat
      961 tgaacgggat actgtcggcg atcaggtagc ggtacagcgc cgcgccgtcg atattcgtgc
     1021 cgggcgggtt attgatgctg ctcggtaggc cacccagggg tagcgagact tgttgccctc
     1081 cattccagta attcccaccc agtccccatt cttccgtgcc ggttgccgcg atctggggtg
     1141 tcaggctatc atcaagatag atgtgtggat cgccttccag cgtcccgccc ggcgtggtaa
     1201 agttgatgac cgtgccgacc agcttgccgc tgccctgcac atccagaaag gtcatatcct
     1261 gtccaaatgt gggattggcc acgctgctat aggtcgcatg gaacgtcccc caccactgcg
     1321 gcggatcggg aaacggctca taacccacgt tccactgcac gccgttgagc ggagcatcgg
     1381 ccagcaaagc gatgcgcgcc tgcgaagaaa agggcatagg ccagtacaga tgcacgtcca
     1441 ttgccccgct ttgattctgg ctgataccgg ccatcaatcc ctgcaccagc gagcgatttt
     1501 ggggaaaata gatgcccgcg ccgtcgcccg ccaggaattt gataggagca tcgacgctgg
     1561 gctgtgactc gccatcccaa tagatcagca ggcgcgcgtt gccgaacgct accatttcct
     1621 gcggggacac ctggaaatcc agcgcgcgaa tttgtgccgg accgctgagc gtcaccaccg
     1681 gcgtttgctt attagcagta aaagacagag taccgcgctg ttgagcgatc gcttgaggcg
     1741 cgatatcgcc cccggcgcag cgtaaaagcc cgacgacatc attcaccggc tcgctgccat
     1801 tccaggtttg tagcgcagcg ccgtagggca acttacgata gagcgcgtag aaattgccgt
     1861 tggcgtgctg cgatgcccat tgcatactct gctgaaaggg aatggatgtc gccacgatgc
     1921 tgctgccctg gctgatctgc ggattcaagg aaagcgggta agggaaatcg cttgcggggc
     1981 tgctgcccgg attggtctgc ccaagatcac ttgagttcac ggtcgtgatg gcatgtccat
     2041 ccagcgagag atgccagggg ccgccgtagt tttcctgcat ccgcataaac gtcatgatac
     2101 ccggtcccaa ctggtcgaaa aggacatgct caccgtccgg taagacacgc gcgacgtgcg
     2161 tataatcagc attgctgccg tcgggatcgg cggtggacgc gccctcaacg cggtctccca
     2221 gttccaggta cgaaagttta tccaggtggc ggtatgcgtc caggccgaga taaagcggtg
     2281 gcgttgtccg cgtttttacg gtgactgctg gctgcgctcc cagggtggct gcgcacgtat
     2341 ccgtgaccac acccgcctgg ttaaaaggcc agtaacgtgc ggcgaccagg acaaccgcta
     2401 gcaacatgag tatgagcagg gcaatcagga gtatgcgttg tttcgcgtcg gaattgtgtt
     2461 tatcagtcat ctatccctgt ttcggtaaga aattgtcacc ctgag
//
LOCUS       DASZAK010000072         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_37137, whole genome shotgun sequence.
ACCESSION   DASZAK010000072 DASZAK010000000
VERSION     DASZAK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37137"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGT44_01135"
     CDS             complement(<1..224)
                     /locus_tag="VGT44_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579428.1"
                     /translation="MLARALYLEGRSSLNAALYASMTAHSLREGIEALVHAYLGWFEQ
                     HADLGQYLLQAADSEYLSAYIKVLRQKTRTT"
     gene            602..>2319
                     /locus_tag="VGT44_01140"
     CDS             602..>2319
                     /locus_tag="VGT44_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579429.1"
                     /translation="MQTGAQRGVGQNAGGRERLARRNRPVGPHYKWVALTNTTIGVLM
                     ATIDSSIVLISLPVIFKGIHIDPLAPGETGYFLWMLLGYMVVTATLLVTFGRISDMFG
                     RVRFYNLGFLIFTIGSILLWLTPGTGSAGALELILFRFVQGIGGGFLLSNSAAIITDA
                     FPAHQRGFALGINQIAAILGSVIGLILGGILAYFDWRLVFLVSVPVGIAGTIWAYTML
                     RETAKPRRNQKIDWLGNVTFATGATVLLIGITYGIEPYGGSSTGWGNPLVIAALILGV
                     LLLATFIWIELHVKDPMFRLELFKIRMFAAGNASSFLASVARGGLQFMLVIWLQGIWL
                     PLHGYNYEVTPLWAGIYMLPLLVGFLLMGPLSGWLSDRYGARPFSTLGLLLQALGFFL
                     LTIIPLNFQYIWFALILFIVGIGQGMFSSPNTSSIMGSVPPEQRGVASGMRATLQNSG
                     TIVSLGVFFSLITSGLAASLPTTLASGLTNAGVPAGLAARLAHLPPTSALFAAFLGYN
                     PIGTLLSPIVLQALPAANRAVLLGHEFFPNLISYPMSIGLHSVFYLSVVMCLVAACAS
                     VLRGRA"
BASE COUNT          411 a          628 c          637 g          643 t
ORIGIN      
        1 gtggttctgg tcttctgacg gagtactttg atatacgcgc tcaaatattc tgaatcggct
       61 gcttgcagga gatattgtcc gagatcggca tgttgttcaa accaaccaag ataggcatgg
      121 acaagcgctt caatgccttc acgcagggaa tgcgcggtca tggaggcgta aagcgccgcg
      181 ttgagactac tccgcccttc gaggtagagc gcgcgtgcaa gcatctcttt atttgagaag
      241 tgatgataaa tacttcctgt actggcccct gatatcttcc tgatatcttc catggttgtc
      301 tcagtgaaac ccttgctcgc aaaacaggta agcgctgctt ccaatatcgc ctgcctccgg
      361 gattctgtcg tcgtactctg cccggtatcg tcaattacca tgatgcaaca agcctcttct
      421 ctctctgtac gtgcggctgc gctaccactc tctgccctct ctgtccctgt tcctgaaggt
      481 gtttgtcaat tataacacgc cccttgaccg taatcaaatt ctagaatata attctagtga
      541 tgggtgcctg cgaaccatcc atcatcgaag agcagacttt tatggaagga atgaggcgcg
      601 tatgcagact ggcgcacagc gtggggtcgg gcaaaatgcc gggggacgcg agcgattagc
      661 gcggcggaat cgacctgtcg gcccacatta taagtgggtg gcgcttacca atacgaccat
      721 tggcgtctta atggcgacga tcgattcaag tattgtcttg atctcgttgc cggtgatctt
      781 taagggtatt catattgatc cactcgcgcc aggtgagacc ggctattttc tctggatgct
      841 actgggctac atggtggtga cagcaacgct cctggtcact ttcggtcgta tctccgatat
      901 gttcggacgt gtgagattct acaacctggg atttttgatc tttacgattg ggagtatcct
      961 gctctggctc acccctggca ccggcagtgc gggggcctta gaattgattc tcttccgttt
     1021 tgtgcagggg atcggcggcg gttttctcct ctcgaatagc gcggcgatta ttacggatgc
     1081 gttccctgcg caccagcgcg gcttcgcgct tggtatcaac cagatcgcgg cgattctcgg
     1141 ctcggtcatc gggctgatcc tgggcggtat tctggcgtat ttcgattggc gcctggtctt
     1201 tctggtcagt gttccggtcg gtatcgccgg cacgatctgg gcctatacga tgctgcgtga
     1261 gacggcaaag ccgcgtcgta accagaagat cgactggttg ggcaatgtta ctttcgcgac
     1321 cggcgcgacg gtcctgctca tagggatcac ctacgggatc gaaccctacg gcggttcatc
     1381 gactggttgg ggtaatcccc tggtgatcgc tgctctgatc ctgggcgtcc ttctgctggc
     1441 tacgttcatc tggattgaac tccatgtgaa agatcccatg ttccgcctgg aactttttaa
     1501 aatccgcatg tttgctgccg gaaacgccag tagtttcctg gcaagcgtcg ctcgcggcgg
     1561 tctccagttt atgctcgtca tctggcttca aggcatctgg ttgccgctgc atggctacaa
     1621 ttatgaagtg actcccctgt gggcgggtat ctatatgctc ccgttgctgg tcggattctt
     1681 gctgatgggg ccgctgagtg gctggctttc ggaccggtac ggcgctcgtc cattctcgac
     1741 gttgggcctg cttcttcaag cgctcggttt tttcttgttg acgattatcc cgctgaactt
     1801 tcaatacatc tggttcgcgt taatcctctt catagttggt atcggacagg ggatgttctc
     1861 ttcaccaaac acgtcgtcga ttatggggag cgtccccccg gaacagcggg gtgtcgcttc
     1921 tggtatgcgc gcgacgcttc agaactcggg taccatcgtg agtctcggcg tcttctttag
     1981 tctgattacg tcgggcctgg ctgcaagttt gcccacgacc cttgccagcg gcctgaccaa
     2041 tgccggggtt ccggctggtc tggcggccag gcttgcgcat ttgccgccga catcggcgct
     2101 cttcgctgct tttctgggct acaatccgat agggacactg ctctcaccca ttgtactgca
     2161 agccttgcca gcggcgaatc gagctgtcct tttaggtcat gaattctttc ccaatctcat
     2221 ctcgtacccg atgtctatcg gcttgcacag cgttttctac ctctccgttg tgatgtgcct
     2281 ggtggctgct tgcgcctctg tactgcgtgg cagagccag
//
LOCUS       DASZAK010000073         5759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_214843, whole genome shotgun sequence.
ACCESSION   DASZAK010000073 DASZAK010000000
VERSION     DASZAK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214843"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..985)
                     /locus_tag="VGT44_01145"
     CDS             complement(56..985)
                     /locus_tag="VGT44_01145"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2579430.1"
                     /translation="MLDPGAMRTSVEWRQAEPQDAAIWVSVTRRSADQTSLESEDAQL
                     VRYREESVEQQAQRYLLWRDDMPVGRLRFFTEGNTAVLDGLVLIPEAGGSVAAQVVTE
                     ATLRAAALEVRHLKVTYPAAYIASFASAGFQELRRRTGMVASTAITMPQVVLPASLRV
                     RPIAGDDTDQIGTLLQRAYAGGPDEIHPDISGWRAEVRAVQEGRFGPFLPESCFVVEH
                     TLDRFNLAGAVLVHLERNIPRIRHMAVAPLFRHVGLGQLLAVKAMRQLRDMDYATVML
                     YVTLGIPAVNLYHRLGFVEAGATYIEAERMLSR"
     gene            complement(1110..1442)
                     /locus_tag="VGT44_01150"
     CDS             complement(1110..1442)
                     /locus_tag="VGT44_01150"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEV2579431.1"
                     /translation="MQLEEYFDFLSEDDIRLKGTRIGIETVLYDFIHRQHSPETIAQT
                     YPSLTLEQVYATILYYLHNKEAVSKYLADWLEWGREVRKQQNQNPSDAARRLRRIKEE
                     RQAYKETA"
     gene            1639..2295
                     /locus_tag="VGT44_01155"
     CDS             1639..2295
                     /locus_tag="VGT44_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579432.1"
                     /translation="MELLVATLAHLQKGNYDDAVSRLRFIVDTVKNAQGMMNARLYCG
                     KEGDGYSLILTAWENEETWQKAQERYNPRELLLQSTGDLLSAAPEQWLMHYLWGYSRP
                     AAQPLIAEAHLATVQPDAANRIAQQWLDELRRQALSPTLAFAFLARGTRGKNEDTTLY
                     QAPGVNGSRSAYSYTFLNLLSWPGESHRKEFYADQHYKALQSSLTNVGMVRVLTLEPS
                     "
     gene            complement(2299..2712)
                     /locus_tag="VGT44_01160"
     CDS             complement(2299..2712)
                     /locus_tag="VGT44_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579433.1"
                     /translation="MDQDAQDLEYVLSEEADEARRLAVRENARRVMRESGMAEMLRTL
                     NTNALKGRGTFHEYDSMVLLRWGTSTTRRHLWIEVSGNTIRFRLTPHRRCTAPTPICD
                     GEYHTFTGPMWADRAFLLRELDKYFAKPVAETSSD"
     gene            complement(2718..3806)
                     /locus_tag="VGT44_01165"
     CDS             complement(2718..3806)
                     /locus_tag="VGT44_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2579434.1"
                     /translation="MANILVVEDERVIARLLKESLQIEGFQVVTVLNGEDAIQFALRE
                     TPHLIILDLALPGLDGYEVARCLRTHHKTMHIPIIALSSYGELADKVRAFEIGVDDYI
                     TKPFQIDELLARIRTQIRRVQQNYLSPLIGLPGGLQVELAIKQKLNCPEPWSILYLDL
                     DNFKAFNDVYGFLTGNDIIRLVGRICQRVVRDYGNAYDFVGHIGGDDYVVVTTPDRAS
                     MLCKQISTAYKEESAVYYRPEDLRRGSISGVDRKGRVYQFPLVSLSIGVVNNQGRRSH
                     SLEEVSYLAAEAKYYAKQSTSNVYHISPRRDVIGPGRPVSYPSTPPYLPPLPAQYTAA
                     NPHTGALGETDFYSTVEAGGRAEYEQRV"
     gene            4315..4998
                     /locus_tag="VGT44_01170"
     CDS             4315..4998
                     /locus_tag="VGT44_01170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2579435.1"
                     /translation="MSTHLAPVERVVTFPSRGQPSFQLEGIVQAPADLGRDGLAPVVV
                     LCHPQPASSDMRDTLTAMLARQLAESGMIALRYNFRGVGKSQGQQSDGRMEPLDLAGA
                     VDCALAQPGANPAKVCVIGHGFGAFIALLYAPFDPRIRTLVAVSLPLFRAAGGFPRPF
                     ERPKLFVTGEFDELCPLYKLEPFVEQLKGAKGIKVIVGARHLMREHEEAAVTAIVKYI
                     TNWAEMPGV"
     gene            5008..>5759
                     /locus_tag="VGT44_01175"
     CDS             5008..>5759
                     /locus_tag="VGT44_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEV2579436.1"
                     /translation="MNPNNPLIVQSDRTVLLEVDHPLHAEARDALAQFAELEKSPEHI
                     HTYRLSPLSLWNAAAGGLDASTILDSLARYSKYDVPPNIIADIREYISRYGRVKLIRD
                     GEMLLLTSNDVALIAEIMHHKRTQPFLLRQIDKRTIQVDASRRGHIKQALIHIGFPAE
                     DLAGYTDGSPLPMKLRGETLGGMAFAPRAYQYAASAAFYAGGAPSGGSGVVVLPCGAG
                     KTIVGMATMADIQRATLILTPNTVAVRQWIQE"
BASE COUNT         1299 a         1631 c         1604 g         1225 t
ORIGIN      
        1 tttaatcgta ttctaaattg cgctaattcc cctctaatat ttgtgctgtt gattcctaac
       61 gggagagcat tcgctccgct tcgatatagg ttgcgccagc ctcgacaaaa cccaggcggt
      121 gatagaggtt gacggcgggg atgccgagcg tgacgtacag catgaccgtt gcgtaatcca
      181 tgtctcgcaa ttgccgcatg gctttcaccg ccaggagttg tcccaggccg acatggcgga
      241 agagcggcgc aacagccata tgccggatgc gcgggatatt gcgttccagg tgaacgagaa
      301 ctgcgcccgc caggttgaat cgatcaagcg tatgttcgac cacaaagcag ctttcaggca
      361 aaaacggacc gaagcgtccc tcctgcactg cgcgcacctc ggctcgccag ccgcttatat
      421 cgggatggat ttcgtctggc ccgcccgcgt aggcgcgttg caacagagtg ccgatctgat
      481 cggtatcatc cccggcaatt gggcggaccc gcagcgacgc aggcagtact acctggggca
      541 tcgtgatagc ggttgaggcg accatacccg tgcgccggcg aagctcctga aagcctgcag
      601 aggcaaacga ggcaatataa gcagcaggat aggtgacttt cagatgcctg acttcgagcg
      661 ccgccgcgcg gagcgtggcc tcggtgacga cctgggccgc gacgctgccc cccgcttcag
      721 gaatgagtac caggccatcc agcactgcgg tgttgccctc cgtaaagaag cgaagccgtc
      781 ccaccggcat gtcatcgcgc cagagcaggt agcgctgcgc ctgctgctcc acgctctctt
      841 cccggtatcg aacaagctgc gcgtcttcgc tctctaacga agtctggtcc gcgctgcgcc
      901 gcgtcacaga aacccagatg gcggcatcct gcggctcggc ctgcctccat tccacggaag
      961 tacgcattgc accagggtca agcatgggaa tagctccttt ttttacgaca catcaggtaa
     1021 caggtaaata ctcaatgtga tcctcgtatt catgaggagt tgcgccttgt agaggggttc
     1081 aaggttctca tcaataagat agcgaatcgt catgcagttt ctttatatgc ctgcctctcc
     1141 tctttgattc gacggagcct cctggccgcg tcggaaggat tctggttttg ttgcttacga
     1201 acttctcttc cccattccaa ccagtcagca aggtactttg aaaccgcttc tttattatgc
     1261 aggtagtaca gtatagttgc gtaaacctgc tctagagtca gcgagggata tgtctgggca
     1321 atcgtttctg gagaatgctg gcgatgaata aagtcataca ggacggtctc tatgccgatc
     1381 ctggtacctt tgagcctgat atcatcctct gataggaaat cgaagtattc ttcaagttgc
     1441 atatggtgcc tccatacttc gtattgttac tggagttttt gagcaaaagg ctattaccac
     1501 ttgctagcat gtagcatacc atagcaaata cctcactgca atccaatcat ttcattgaca
     1561 aaaccctcaa gctatgacat cctacgatac ggtacaacac aaagagcaaa tatgggtgaa
     1621 agagaagcaa taagaagtat ggaactactg gttgcgacgc tggcacattt gcagaagggc
     1681 aattacgacg acgcggtaag ccggctgcgc tttattgtgg ataccgtaaa gaacgcgcaa
     1741 ggcatgatga atgcgcggct ctattgcggg aaagagggcg atggatatag tctgatactc
     1801 acggcatggg agaatgaaga aacctggcag aaggcgcagg aacgctacaa tcccagggaa
     1861 ctcctgctgc aatccacggg cgatctcctc tctgccgcgc ctgagcaatg gctgatgcat
     1921 tacctgtggg gctacagccg cccagccgcc cagccgctca tcgcggaggc gcacctggcg
     1981 acggtgcagc ccgatgccgc caaccgcatc gcgcagcaat ggctcgacga gctgcgccgc
     2041 caggcgctct cgccaacgct ggccttcgcg ttcctggcgc gcggtactcg gggcaagaat
     2101 gaagatacca cactctatca agctcccggt gtgaatgggt cgcggtcggc ctacagctac
     2161 accttcctga acctgctgag ctggcccggc gagtcgcatc gcaaggaatt ctacgctgac
     2221 cagcattaca aggctctgca aagctctctc acgaacgtcg gcatggtgcg cgtcctcacc
     2281 ctcgaaccgt cgtagatatt aatccgagga ggtttccgcg acgggtttgg caaagtattt
     2341 atcgagttcg cgcagcagga aggcgcggtc agcccacatc gggccggtga aagtatggta
     2401 ttcgccatcg catatgggcg tgggggcggt acagcggcgg tgcggtgtca ggcgaaaacg
     2461 gatggtgttc ccgctcacct cgatccagag atggcgacga gtcgtactgg tgccccagcg
     2521 cagcagcacc atcgagtcgt attcgtggaa ggtccccctg cctttcaaag cgttggtatt
     2581 gagtgtacgc agcatctcgg ccatgccact ttcacgcatg acacgcctgg cgttctcacg
     2641 cacagcaaga cgacgcgcct cgtccgcttc ctcgctcagc acgtattcca ggtcctgcgc
     2701 gtcttgatcc attgctctta tactcgctgc tcgtattccg ctctgccgcc cgcctcaact
     2761 gtcgagtaga aatcggtttc accaagcgcc cccgtgtggg gattagcagc ggtgtactga
     2821 gctggcaagg gcggtaaata tggcggggta gagggatagg aaactggccg accggggcca
     2881 atcacgtcgc gacgcggcga aatatgatag acgttgctgg tcgattgctt tgcgtaatac
     2941 ttggcttccg ccgcaaggta gctgacttct tccaggctat gcgagcgacg cccctggttg
     3001 ttgacgaccc cgatggaaag agagaccagc ggaaactggt acacgcgccc tttgcgatca
     3061 acgcccgaaa tcgagccgcg tcgcaggtct tcaggacggt aataaaccgc gctttcctct
     3121 ttataggccg tgctaatctg tttgcagagc atactggcgc gatcaggcgt ggtgacaacc
     3181 acgtaatcgt cgccgccgat atggcccaca aaatcgtagg cattgccgta atcgcgcacg
     3241 acgcgctggc agatacgacc taccaggcga ataatgtcgt tgccggtcag aaagccgtag
     3301 acatcattga aggctttgaa gttatcgaga tcaagataga gaatactcca gggctccggg
     3361 cagttgagtt tctgcttaat ggctaattca acttgcaggc cacctggcag gccgatgagc
     3421 ggggacaggt aattctgctg aacacgacga atctgggtac gaatgcgcgc gagcaattca
     3481 tcgatctgga atggttttgt gatatagtca tcgacgccaa tctcaaaggc gcgaaccttg
     3541 tcggccagtt caccatagga actcagggcg ataatgggaa tatgcatcgt tttgtggtgt
     3601 gtgcgcaaac atcgcgctac ctcgtaacca tccaaccccg gcagcgccag gtcaagtatg
     3661 atgagatgtg gtgtctctct cagcgcaaac tgtatcgcat cttcgccgtt aaggacggtc
     3721 actacttgaa acccctcgat ctgtagcgat tcctttagca atcgggcaat cacccgctca
     3781 tcctcgacga ctaaaatgtt ggccataaac actcctgcct cttgaaatat agggaaaacc
     3841 cttgatacaa gccgcgtact ccaggcacgc cacacgctac gtaaccaccc accacgccac
     3901 tgcgctcttt agcaacgctg cattgtccac aagaagcctg ataaaatcct tcccccctga
     3961 aggattccat gtaggcgtta gccgcgatga gaggggttcg aaaatacagg gatcgcgcga
     4021 cgaacctctc tcaggatttt actgtgtcta aaaaaatcag acctccgtac aaatacgtag
     4081 gttgtacatg ttcatcattc agtataacat ccttatgccc agatgcaaag ggcttcaccc
     4141 gacaaattcg cgaaatgtat gtaaagtgtg ttgatttgtg acaaattcgt ctcgccgggg
     4201 caacaacgag gggtactcta ggggagattc gcgtggagca gttgacgcat cgctgtatct
     4261 gcgataagat gaaatgagct tctggtgagt gagtgagtag gaaaaggcga acgcatgtca
     4321 acgcatctgg caccggttga gcgagtggtg acattcccca gtcgcgggca accttctttt
     4381 cagttggagg gcatcgttca agcgccagcc gacctgggca gggatggact tgctccagtt
     4441 gtggttcttt gtcatcccca accagccagc agtgatatgc gcgatacgct aacagcaatg
     4501 ctggcgcgac aattagcgga atcgggcatg atcgcgctgc gctacaattt tcgcggcgtt
     4561 ggcaagagcc aggggcagca aagcgatggc cgcatggaac cgctcgacct ggcaggcgcg
     4621 gttgactgcg cgctggctca gccaggagca aatccggcca aagtgtgtgt gatcggtcac
     4681 ggctttggcg cgttcatcgc cctgctgtac gcaccgttcg atccgcgcat tcgcacactg
     4741 gtcgccgtca gcctgccctt attccgcgcc gccggcggct ttcccaggcc attcgagcgg
     4801 ccaaagctgt ttgtgaccgg cgagttcgat gaactctgcc cgctctataa gctggagccg
     4861 ttcgtagagc aactcaaggg agcgaagggc attaaagtga ttgtgggagc gcgccacctg
     4921 atgcgtgaac atgaagaagc agctgtaacg gctatcgtaa aatatataac caactgggcg
     4981 gagatgcccg gagtatagag ataacacatg aatccgaata acccattgat cgtgcaaagt
     5041 gaccgtaccg tcttgctcga ggtcgatcac cccttacatg ccgaagcgcg cgatgcgctg
     5101 gcacagttcg cggaactgga aaaatcgcct gaacacatcc atacctatcg tctctcgccg
     5161 ctttcgctct ggaacgccgc cgcgggtggg ctggatgcgt caaccatcct ggattcgctg
     5221 gcgcgctaca gcaaatacga cgtgccgccc aacatcatcg ccgatatccg cgaatatatc
     5281 agccgctatg ggcgcgtcaa gctcattcgc gacggtgaga tgctgctgct gacctcgaac
     5341 gatgtcgcgc tgatagcgga aattatgcac cacaagcgca cgcagccctt cctgctgcgc
     5401 cagattgaca agcgtactat ccaggttgac gcctcgcggc gcggacacat caagcaggcg
     5461 ctgatccaca tcggctttcc cgccgaagac ctggcgggct ataccgatgg ctcgccgctg
     5521 cccatgaagc tgcgcggcga gacactcggc ggcatggcat tcgccccgcg cgcctaccag
     5581 tacgcggcct cggctgcctt ctatgccgga ggcgcgccca gcggcggcag cggcgtggtc
     5641 gtgctgccct gcggcgcggg caaaacgatc gtcggcatgg caactatggc cgatatccag
     5701 cgcgccacgc tgatcctgac acccaacacg gtcgcggtgc gtcagtggat tcaagagat
//
LOCUS       DASZAK010000074         1589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_285637, whole genome shotgun sequence.
ACCESSION   DASZAK010000074 DASZAK010000000
VERSION     DASZAK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285637"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..>1589
                     /gene="lysS"
                     /locus_tag="VGT44_01180"
     CDS             282..>1589
                     /gene="lysS"
                     /locus_tag="VGT44_01180"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV2579437.1"
                     /translation="MADEREIRIQRLHALRERDIEAYPNTAERTHTVTEVLEHFDEWQ
                     GESGEFTLVGRIRLLREMGKAAFAHIEDGTGRIQIFFRINDLGEEAYRAIKLLDIGDF
                     IQVTGFLFRTRTEERTLHVRSYRLLAKSLRPLPEKYHGLEDVEIRQRKRYLDLLANGE
                     EARQVFIIRSRTISAMRRYLDDHGFIEVETPILQPLYGGATARPFTTHHNTLDRDLYL
                     RIAVELYLKRLIVGGFERVYEIGRDFRNEGIDRSHNPEFTMMECYQAYADYNDIMRLV
                     EEMVHSIALEVKGSSRITYQGTSIDLTPPWPRIRLLDAIAEFTGIDVERCPDRESLAA
                     AMRANGYEADPKLGRGRLIDDLKGTMLRMGIPALKQALFLIDYPLDVSPLTKRHREIS
                     GLTERFQPFAGGLELGNAFTELNDPLDQRARFEDQMRQLAQGDD"
BASE COUNT          356 a          496 c          447 g          290 t
ORIGIN      
        1 cgccacctgg gcgcaatgaa ttgcgcccct acacgccatt tgaaccttga caatgtagta
       61 ggagccgatt gatcgcgctc gcagccgatt gatcggcgcg aatctccggt tatgggtttg
      121 gactggccga taaatcggct cctacgagag attatcggca atacatacag aaaaccggcc
      181 cctacgggct attgtaagac acatggaata gccaatttat gcaaacaatg ttacaatggt
      241 tcgcacacaa atggcatata tggaggaata ggaaacaacc aatggcagac gaacgagaaa
      301 tacgcataca acggcttcat gccctgcgcg aaagggacat cgaggcgtat ccgaatacag
      361 cagaacgtac gcataccgtc acggaggtac tggaacactt cgatgaatgg cagggagaaa
      421 gcggcgaatt tacgctggtt ggacgcattc gcctgttgcg cgaaatgggc aaggccgcct
      481 tcgcgcatat cgaggacggc accgggcgca tccagatctt cttccgcatc aacgacctgg
      541 gcgaggaagc ctaccgcgcc attaagctgc tcgatatcgg cgattttatc caggtcaccg
      601 gcttcctgtt caggacgcgc accgaagaac gcaccctgca cgtgcgctcc taccggctgc
      661 tggcgaagtc gctgcgcccc ctgccggaga agtatcacgg gctggaggac gtggagattc
      721 gccagcgcaa gcgctacctc gacctgctcg ccaatggcga agaggcgcgg caggtcttca
      781 tcatacgcag ccgcacgatc agcgccatgc gccgttacct cgacgatcac ggcttcatcg
      841 aggtcgagac gcccatcctg cagccgctct acggcggggc gaccgcgcgt ccattcacca
      901 cgcatcacaa cacgctcgac cgcgatctgt acctgcgcat cgccgtagag ctatacctca
      961 agcgcctgat cgtaggcgga tttgagcgcg tctacgaaat tggccgcgat tttcgcaacg
     1021 agggcatcga ccgcagccac aatcctgaat ttaccatgat ggaatgctat caagcctacg
     1081 ccgattacaa cgatatcatg cggctggttg aggagatggt ccactctatc gcgcttgagg
     1141 tcaaggggtc gtcgcgcatc acctaccagg gaacgtcgat cgacctgacc ccaccctggc
     1201 cacgcattcg cctgctcgac gccatcgccg aattcaccgg catcgatgtc gagcgctgtc
     1261 ccgaccgcga gagcctggcg gctgccatgc gcgccaacgg ctacgaggcc gatccgaagc
     1321 tggggcgtgg ccgcctgatc gatgatttga aggggacgat gctgcgtatg ggcattcccg
     1381 ccctcaagca ggcgttattc ctgatcgact acccgctcga tgtctcgccg cttaccaaga
     1441 ggcaccgcga gatttccggc ctgaccgagc gcttccagcc cttcgccggc ggcctcgaac
     1501 tcggcaatgc cttcaccgag ctgaacgacc cactcgacca gcgcgcgcgt ttcgaggacc
     1561 agatgcgcca gctcgcgcag ggcgacgac
//
LOCUS       DASZAK010000075         2183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_321483, whole genome shotgun sequence.
ACCESSION   DASZAK010000075 DASZAK010000000
VERSION     DASZAK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321483"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VGT44_01185"
     CDS             <1..424
                     /locus_tag="VGT44_01185"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEV2579438.1"
                     /translation="NVEALLREQLGRSGPCHTHRDQYVAIPGKPPVVPDVVLTCDRAD
                     WDKDKRLKPFKIQSPLIVVEVLSPSTERYDRTEKFARYMSCPTLEVYILVSQDEQHIE
                     VYRRSRDWKQERFSVDQTIRLEQHDLELPVESIYEGVF"
     gene            475..1542
                     /locus_tag="VGT44_01190"
     CDS             475..1542
                     /locus_tag="VGT44_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2579439.1"
                     /translation="MKASFLPTTTYGVRAVYEAGWPSPPGLFEPELGRRMMEEMFEQS
                     ALADELGFDWISFSEHHYAPLLVPTPNPAIFAAAAMQRIRRAKIALFGPTLPLHNPIH
                     VAEEVAMLDVLSGGRLLLLFLRGVPYELRAFSVNLDETRAMTQEATLLITRALTDAQP
                     FSWEGKYYRYQTVSVWPGLIQRPHPPFYSTGNSFESATFAASQGHGLALSFAPLSYVA
                     QVIPHYRAEVAKHGWGPRDDQIVFRGNVIATDTDEEAQELAVQLAALPHLKRPQSPPA
                     AAPANLEKVSPVAPVLIGSAQFCGSPPTLVAQMKALHDLGVSVVDLVIPTGLMSQAQV
                     LHSLELLAREVLPRIHDFGGD"
     gene            complement(1492..>2183)
                     /locus_tag="VGT44_01195"
     CDS             complement(1492..>2183)
                     /locus_tag="VGT44_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV2579440.1"
                     /translation="GRIREAELHAATEVLGVRSVDFLGYLDGELDQAHPAEVVAKIVG
                     HLRCIKPNVVVTFGPDGSYGHPDHIAISQFTTAAIVEAASPNSLYERDLAPHRVAKLY
                     YMAHTKDLLDVYQLVFGDLVMHIDGVERRGAGWPDWQITTKIDTWTSWRTVWKAVCCH
                     QTQLPAYHLLEGLPEEQHKVLWGCQTFYRAFSLVNGGRTIERDLFEGLRLKGASIPTE
                     IVDARKYLSRE"
BASE COUNT          440 a          642 c          612 g          489 t
ORIGIN      
        1 taacgtcgag gctctgttgc gcgaacagct gggcaggagt ggtccgtgtc atacccatcg
       61 agatcaatat gtggcgatcc caggaaagcc cccagtcgtc ccggatgtag tgctgacgtg
      121 tgatcgagca gattgggaca aagacaagcg gctcaaacct ttcaagattc aatccccact
      181 catcgtcgtt gaagtgcttt cacccagcac cgaacgatat gatcggacgg agaaatttgc
      241 tcgctatatg agctgtccca cgttagaggt atatatcctg gtgagtcagg acgagcagca
      301 tattgaggta taccggcgtt ccagggattg gaaacaggaa cgcttttctg tcgatcagac
      361 gatcaggcta gagcaacacg atctggaact gccagtcgag tcgatctatg agggtgtgtt
      421 ttagcatctc actttaccct tctcgaattc ctgggcggtg aaagagagta tggtatgaaa
      481 gcttccttct tgccaacaac cacctatggc gtccgagcgg tctatgaagc aggctggcct
      541 tctcctcccg gcctgtttga gccggagctt ggtcgtcgca tgatggagga gatgttcgag
      601 cagtccgcgc ttgccgatga actaggcttt gactggatca gcttttcgga gcatcactac
      661 gcgcctttgc tggtgcctac ccccaatccc gctatcttcg ccgcagcagc gatgcagcgc
      721 atccgccggg cgaaaattgc cctgttcgga ccaacgctgc ccctgcacaa tcccatccat
      781 gtcgccgaag aggtcgccat gctcgatgtt ttgagcggcg gacgcctgct gctgcttttc
      841 ctgcgcggcg tgccttacga actgcgcgct ttctccgtca atctggatga gacgcgcgcc
      901 atgacgcagg aggccacgct cttgatcacc cgggcgttga ccgacgcgca gccgttttcc
      961 tgggagggca aatactaccg ctatcagacc gtatcggtct ggcccggcct gattcagcgt
     1021 ccccatccgc cgttctattc gacgggcaac agctttgagt cggccacgtt tgcggccagt
     1081 cagggccatg gactggctct atccttcgcg cctctctctt acgtcgccca ggttatccct
     1141 cattacagag cagaggtcgc caaacacggg tgggggcctc gagacgacca gatcgtcttt
     1201 cgtggcaacg tcatcgcgac tgacaccgat gaagaagcgc aggagctggc ggtgcagctc
     1261 gccgcgctgc cccatctcaa acgcccgcaa agccctcccg ctgccgctcc cgccaatctc
     1321 gaaaaggtca gtccggtcgc tcctgttctg atcgggtcag cccagttctg cggaagcccg
     1381 ccaacgctgg tagcgcagat gaaggcgctg cacgacctgg gcgttagcgt ggtagacctg
     1441 gttattccca ccggtctcat gagtcaagcg caggtgctgc actccctcga attactcgcg
     1501 agagaggtac ttccgcgcat ccacgatttc ggtggggatt gatgctccct ttaaccgcag
     1561 tccttcaaac agatcgcgct caattgtacg tccgccattg acgagactga aggcgcgata
     1621 gaatgtttga cacccccaga gtactttgtg ttgctcctcc ggcaagcctt caaggagatg
     1681 gtaggctggc agttgtgtct ggtggcagca cactgccttc cagactgtgc gccaagaggt
     1741 ccaggtatca atcttcgtgg taatctgcca atcgggccac cctgctccgc gccgttctac
     1801 tccatcgata tgcatgacca ggtcaccaaa gacgagttgg tagacatcca acaagtcctt
     1861 cgtgtgtgcc atgtaataca gctttgcgac gcgatgggga gccaggtccc gctcgtacag
     1921 agagttggga ctggctgctt cgacgatggc ggcggtggta aactgcgaaa tggcgatgtg
     1981 atctggatgt ccatagcttc catctggccc gaaggtaacg acaacatttg gcttgatgca
     2041 ccggaggtgt ccaacgatct ttgccacgac ctcggctggg tgggcctggt ccagttcgcc
     2101 atcgaggtat cccaggaaat cgacgctgcg gacaccgagg acttcggttg ccgcgtgcaa
     2161 ttctgcttca cgtatccgcc cca
//
LOCUS       DASZAK010000076         1817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_108665, whole genome shotgun sequence.
ACCESSION   DASZAK010000076 DASZAK010000000
VERSION     DASZAK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108665"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1760)
                     /locus_tag="VGT44_01200"
     CDS             complement(6..1760)
                     /locus_tag="VGT44_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2579441.1"
                     /translation="MMLDTTITHQRPLPVGITKGPGESTHEYTFVSRDDAQELKNGEY
                     VYYELDELESATIAATASAPASNGSKPGGARRVLGRILKRVPLQLYPDTFLAEPEISP
                     SQVAAMVGYDARANELFELHVAIMGYYDATSASFINPWIPPQSGKQIFLADDLMLANI
                     LSRKHTREPGSATVGSLLTRTPDAVPVVLSVKDVVSTHLAIIASTGAGKSYLASVLIE
                     ELMQPYNKACVLIIDPHGEYSILDQLPNFPQFHEAAEGRRPAYQAVVRVYKPEQVKVR
                     ISSLDMGDMRHLLPEMTEKQQHLLSRALRRVREQKRSTPWGAQDLIGAVKAVSKQKGD
                     EESEGADDSSTVHALEWRIQQRFVDNFTFDDIQHLDLPEIFKPGQCTVLPLNEIDERD
                     QQVVVATLLRRLNRARMDTERGKVHSGESYLPYPVFVLIEEAHHFAPGGAEVVSTAIL
                     KTVLAEGRKFGVGVGLISQRPGKLDADVLSQCQTQCIMRIVNEIDQKSVAAAIEGVGR
                     ELLDNLPALSKGQVIIAGAAVNTPVICRVRPRITRHGGESKDAPDMWRQYFSQESQES
                     RRRHGAPLNGNRDFNLLR"
BASE COUNT          323 a          565 c          543 g          386 t
ORIGIN      
        1 aacactcacc gtaatagatt gaagtccctg ttgccgttca gaggcgctcc gtgacggcgg
       61 cggctctcct gtgactcctg gctgaagtat tgccgccaca tatctggcgc gtccttgctt
      121 tcgccaccat gccgggtgat gcgcgggcgc acgcggcaga tcaccggcgt attgacggcg
      181 gccccggcaa taatcacctg gccctttgac agcgcgggca gattgtccag cagttcgcgc
      241 ccgacgcctt cgatagcggc tgccacgctc ttctggtcga tttcgttcac gatgcgcatg
      301 atacactgcg tctggcactg gctcaacacg tccgcgtcga gctttccagg tcgctgactg
      361 attaaaccga ctcccacgcc aaatttgcgc ccctcggcca gcaccgtctt cagaatggcg
      421 gtggaaacca cctcggctcc gccaggggca aagtgatgcg cctcttcgat cagcacgaaa
      481 acgggatagg gcaggtacga ctcgccagaa tgcactttgc cgcgttcggt atccatgcgc
      541 gccctgttga ggcggcgcag cagcgtggcg accaccacct gctgatcgcg ctcatcgatc
      601 tcgttgagag gcaggacggt acactgtcct ggtttgaaaa tttccggcag gtcgaggtgc
      661 tggatatcgt caaaggtaaa gttatcgacg aagcgctgct ggatgcgcca ttccagcgcg
      721 tgaaccgtgc tggagtcgtc ggcgccctcg ctctcttcgt cgcccttctg cttagaaacc
      781 gctttgaccg caccgatcag gtcctgcgcg ccccacggcg tactgcgctt ctgctccctc
      841 acacgccgca gggcgcggct gagcaggtgc tgctgcttct cggtcatctc cggcagcaga
      901 tggcgcatgt cgcccatatc aagagaggag atgcgcacct tgacctgttc gggcttgtag
      961 acgcgcacaa cggcctgata cgccggtcgg cggccctccg ctgcttcgtg aaattgcggg
     1021 aagttgggca actggtcgag tatgctgtat tcgccgtgcg ggtcgataat caggacgcag
     1081 gctttgttat agggctgcat caactcttcg atcaaaacgc tggccaggta gcttttgccc
     1141 gctcccgtgc tggcgatgat ggcgaggtgc gtgctgacca cgtccttgac tgacaacacc
     1201 accggcaccg catccggcgt gcgcgtcagc agcgagccga cggtggccga ccccggctcg
     1261 cgcgtgtgct tgcggctgag gatattcgcc agcatcaggt cgtcggccag aaagatctgc
     1321 ttgcccgact gtggtggaat ccagggattg ataaacgagg ccgaggtcgc gtcgtaatag
     1381 cccataatgg ccacgtgcag ctcgaacagc tcattggcgc gcgcatcata gccgaccatg
     1441 gccgcgacct gcgaaggcga aatctccggc tcggccagga acgtatcggg gtacaattgc
     1501 agcggcacgc gcttgaggat gcgccccagt acgcgccgcg ctccgcccgg tttgctccca
     1561 ttagacgcag gcgcggaagc agtggccgct atcgttgcgc tctctaattc gtccagttca
     1621 tagtagacgt attcgccgtt cttcaactcc tgcgcgtcat cgcgcgagac aaaggtatat
     1681 tcgtgcgtgc tttcgcccgg ccctttggtg atgcccaccg gcagggggcg ctggtgcgtt
     1741 atggttgtat caagcatcat ggcaagtctc cttaccctgt cgtttccact ttgagccgcc
     1801 aggcaaagtt gcgtttg
//
LOCUS       DASZAK010000077         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_357018, whole genome shotgun sequence.
ACCESSION   DASZAK010000077 DASZAK010000000
VERSION     DASZAK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357018"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..359
                     /locus_tag="VGT44_01205"
     CDS             <1..359
                     /locus_tag="VGT44_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579442.1"
                     /translation="TTFDGKSGLNLALSAFWDGSKWHKVSNPNVAGSNNIFFGVAAVS
                     AKNVLAVGLTFTNTGPDQTFSEQWNGTAWHILTTPDPSGSGTLTAVTRVPGASLYWAV
                     GVSEGGGHNTLIESYC"
     gene            complement(360..806)
                     /locus_tag="VGT44_01210"
     CDS             complement(360..806)
                     /locus_tag="VGT44_01210"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV2579443.1"
                     /translation="MAIRTATSAGAVILREIEGELRIGLAHHDRPGKTWVLPKGHVEA
                     GETLEQAALREIYEETGLEQVQLLVHLGSLVRESLKKDGETEQKTIHYYLAYALEDGQ
                     ATPPPEAGFTYIGWFAPADAVALLPFEAEQAFMRESLPHFFQAQER"
     gene            complement(968..2569)
                     /gene="aceB"
                     /locus_tag="VGT44_01215"
     CDS             complement(968..2569)
                     /gene="aceB"
                     /locus_tag="VGT44_01215"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEV2579444.1"
                     /translation="MTSIKPSLQGVEIMGERTPAFDEVLTPEAIDFVVKLSRAFSGRR
                     EELLQRRARRQAEIDAGKKPDFLPETEHVRNGDWTIAPIPADLQDRRVEITGPVDRKM
                     VINALNSGAKVFMADFEDSFSPVWSEAIQGHINLRDAINRVITYASPEGKQYKLNEQT
                     ATLLVRPRGWHLPEKHFIVDGKAISGGIFDFALYFFHNAKTLLAKGTGPYFYLPKLES
                     HLEARLWNDIFTMAEQELGISHGTIKATVLIETILATFEMDEILYELRDHSAGLNCGR
                     WDYIFSAIKKFRKDPDYILPDRAQVTMTTHFMRSYSLLAIKTCHRRNAHAIGGMAAQI
                     PIKNDPKANEEALEKVRADKRREANDGHDGTWVAHPGLVAIAMEEFDKVMKTPNQIDR
                     KREDVHVTAADLLKIPDGTITEGGLRLNVSVSVQYLESWLRGSGCVPINNLMEDAATV
                     EISRAQIWEWIRHPQGILDDGRKVTIELFRQVLQEEMEKLRAQLGDEQFRKRRFATAA
                     EILDSIITSDQFIEFLTLPAYQYLQ"
BASE COUNT          490 a          745 c          901 g          582 t
ORIGIN      
        1 gaaccacgtt cgatgggaag agtggcttga acctggcgct gagcgcgttt tgggatgggt
       61 cgaagtggca taaggtttcc aacccaaatg tggctggcag caacaacatc ttcttcggag
      121 tagcggcggt gagcgcgaaa aacgtgctgg cagtgggtct gacatttacc aataccggcc
      181 ccgatcagac cttcagcgag cagtggaatg gcacggcctg gcacatactc accactcctg
      241 atccgagcgg gtcgggtact ctgaccgcag tcacacgggt tccgggcgcg tcgctctact
      301 gggcggtagg cgtctccgaa ggcggcggcc ataacacgct gatcgaaagt tattgctagt
      361 tatcgctcct gagcctggaa gaagtggggc aggctctccc gcatgaatgc ctgctccgcc
      421 tcgaagggga gcagcgcgac agcgtcggcg ggcgcaaacc agccaatgta ggtgaagcct
      481 gcttccggcg ggggagtagc ctggccgtct tccagcgcgt aggccaggta gtagtgaatg
      541 gtcttctgct cggtctcgcc gtcctttttg agggattcgc gcacgagcga gccaagatgc
      601 acgagcagtt gtacctgctc aagcccggtt tcttcgtaga tctcgcgcag agcggcctgc
      661 tcaagcgtct cgccagcttc gacatggcct tttggcagca cccatgtctt tcctggccgg
      721 tcgtggtgag ccagcccgat gcgcagttcg ccctcgattt cgcgcaggat gacggctcct
      781 gcggatgtgg ctgttcttat agccatcgtt ataccttcct gatgcgacaa tgacgaatgc
      841 gggcgaccag gtacgcgaga cggatcgcga tgccgactgg gacgagggcg acgcaagcgt
      901 ccccgccctg ccccgcgtcc gcccccgccc gtacagaagg cgtgggacgg acgtggggca
      961 acgggcgtca ttgcaggtat tggtaggcgg gcagggtcag gaactcgatg aactggtcgc
     1021 tggtgatgat cgagtcgagg atttcggcgg cggtggcgaa gcggcgtttg cggaactgct
     1081 catcgccgag ttgggcgcgc agcttctcca tttcctcttg caagacctgg cggaatagct
     1141 cgattgtgac tttgcggcca tcgtcaagga tgccctgggg gtggcgaatc cattcccaga
     1201 tctgggcgcg cgaaatctcg acggtggcgg cgtcttccat caggttgttg atgggaacgc
     1261 agccggagcc gcgcagccac gattcgaggt attgcacgct gacgctgacg ttgaggcgca
     1321 ggccgccctc ggtgatcgtg ccatccggga ttttgagcag gtccgcggct gtgacatgca
     1381 cgtcctcgcg cttgcggtca atctggttgg gcgtcttcat aaccttgtcg aattcttcca
     1441 tggcgatggc caccaggccg ggatgggcga cccaggtacc gtcatgcccg tcgttggctt
     1501 cgcgccgttt gtcggctcgc accttttcga gcgcttcttc gttggctttg gggtcgttct
     1561 tgatgggaat ctgcgccgcc atgccgccga tggcgtgggc gttgcggcgg tggcaggtct
     1621 tgatggccag cagcgagtag gagcgcatga agtgcgtcgt cattgtgacc tgcgcgcggt
     1681 cgggcaggat gtaatcggga tctttgcgga acttcttgat ggcgctgaag atgtaatccc
     1741 agcgcccgca gttgaggcca gccgagtgat cacgcagctc gtagaggatt tcatccatct
     1801 cgaaggtcgc caggatggtc tcgatcagca cggtggcctt gatggtgccg tgcgagatgc
     1861 caagttcctg ttcggccatc gtgaagatat cgttccacag gcgcgcttcc aggtggcttt
     1921 ccagcttggg caggtagaag taggggccgg ttccctttgc caggagggtt ttggcgttgt
     1981 ggaagaagta gagcgcgaag tcgaaaatgc cgccagagat agccttgccg tcaacgatga
     2041 agtgcttttc gggcaggtgc cagccgcgcg ggcgcaccag cagcgtagcg gtctgctcgt
     2101 tcagcttgta ctgtttgcct tccgggctgg cataagtgat aacgcgattg atggcgtcgc
     2161 gcaagttgat gtgaccctgg atggcttctg accagacggg cgagaaggag tcctcgaagt
     2221 cggccatgaa gaccttcgcg ccggaattca gggcgttgat gaccattttg cggtcaaccg
     2281 ggccggtgat ctcgacacgg cggtcttgca gatctgccgg gatgggcgcg atcgtccagt
     2341 cgccattgcg gacatgctcc gtttcgggca ggaaatcggg cttttttccg gcgtcgatct
     2401 ccgcctgccg gcgcgcgcgg cgctgcaaca actcctcgcg cctgccgctg aaggcgcgcg
     2461 acagcttcac gacaaaatcg atggcctccg gcgtcaaaac ctcatcaaaa gcgggagtgc
     2521 gttctcccat aatctctacg ccttgcaggg atggtttgat ggatgtcata tgggtgattc
     2581 ctttcgatac tggtcgcgtc ccttgccata attatataac aagggcatgt gttatagggc
     2641 ggtaaggacg aaggaggaca acaatggtga aggagggcca ccagggcgat gggtggcaac
     2701 cagggcgacc gcaagggt
//
LOCUS       DASZAK010000078         6441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_285730, whole genome shotgun sequence.
ACCESSION   DASZAK010000078 DASZAK010000000
VERSION     DASZAK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285730"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(577..873)
                     /locus_tag="VGT44_01220"
     CDS             complement(577..873)
                     /locus_tag="VGT44_01220"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2579445.1"
                     /translation="MYAIYIKHLGYIDLPAIVFYNDENFISLGVLVMVDHNSWLTVED
                     IYEVLGRKVPMDTIRSWIRSKRLPAFKPGRTYLVKREDLERFLRESRTIPDDQP"
     gene            complement(3349..3969)
                     /locus_tag="VGT44_01225"
     CDS             complement(3349..3969)
                     /locus_tag="VGT44_01225"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579446.1"
                     /translation="MPHDNTAGPRTVRAPRGSDITCKGWQQEAAMRMIMNNLDPDVAE
                     RPDDLVVYGGSGRAARSWAAFDAIVRSLQSLEDNETLLVQSGKPVGIFRTHADAPRVL
                     IANSNLVPHWGNWDEFRRLEALGLTMYGQMTAGSWIYIGSQGIVQGTYETFGEAARKH
                     YGGTLKGKFVLTAGLGGMGGAQPSPFKCRSTRPSRSSTCSESIHSA"
     gene            4414..5457
                     /locus_tag="VGT44_01230"
     CDS             4414..5457
                     /locus_tag="VGT44_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579447.1"
                     /translation="MQNDPATDTLSAQSPLLVGPLMALTGVSQNQSPPTLHLASVQGN
                     SGQVTDSYPLQVPPGPGGFQPQLALTYSSSGPNERHAATSPAADEGDGWSLSLGSISE
                     ETYGSTVWYFLNDVAGVGDRLIYNSSTGLYDTQHLSYLKIQQVTSSTTNQPCFDVWDK
                     SGTFNEIGCTSTSLQYWTDSSGQHNYQWDVDKIVAPNEGPNASVYRLMLVTYVQDSAT
                     TNGYTTIRDAALQEIVYGTGTSTSTISKVVGTVDFFYHGPSLLTGTSDSGSILTWITP
                     YGTNYACASSPPVSTPLRCDNPQDDGTEQAPLVMSTLTLDKVVSYVGTDAGTAPVTRR
                     RVMPSAMAMTSRL"
     gene            5454..>6441
                     /locus_tag="VGT44_01235"
     CDS             5454..>6441
                     /locus_tag="VGT44_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579448.1"
                     /translation="MSCTDPLTLAQEYCAGEHLLLSITPTAYQNNTGHALHPFVFGYT
                     GLRTNTYYDSSEKVGTNHYQAQNSWQYLNFFIDLTTGIGESVSWQIAYSNTDGTPDDL
                     TSQGTVDGRYDPLHCATWNDCTGHYAHPDNQSWSEWVVTQISALGTDSSATALTPAAT
                     TYAYVLAKTGTYSGKVYCYPSANNVDKDCVGDNWIPKGNTDWQDYYHAEYQGFAQVWI
                     VSPSQDLTLDQYHSTEGWYTPWSDYQNFEGGSLYLEDRYSGNSLNLTTLPLLSSLYNT
                     YADNANACRSASATYPACEIVLTPTTSIEYNQTQHENPVSVEHSYAYDDYTAS"
BASE COUNT         1502 a         1853 c         1751 g         1335 t
ORIGIN      
        1 tgcgctcact gcaaagctct gcgccgtagt ggtgcgggcg cgctctgtag ctgagtggag
       61 gcgtgaatgg aagagaaatt cgctctcgct ggttctcaat aagcgaaggg gtggcacccc
      121 cgcaccacaa atgttttcaa acgcctttcc tacccatcga tgcccgatct atttgcatgt
      181 caatagcggc cagactggtt catcgccgct tttggcgtat caagatgtac agagcttata
      241 ctacgtaatc tgagcctact ctggacacct cgctactctg ctctttagcg aagcgacaat
      301 gatttgctta gaacttcttt tctaaaaacc attgccaaag ctgaggggat gaggtatagt
      361 ttatccagca gtgtgaccgc ctgccccgag attcgcgcaa cgttttttgc caagcacgcc
      421 cacactgcac gtgtaccgta tgaaacccgc tgtcaggccg atgtagatca agcgagcggc
      481 gagagggcaa ccgcctgcct tccaagcagg ctgtcgcagg atcgggtcct ggactatttc
      541 ggttttcgtc gaattggctg acacacacga tcgcttctat ggttggtcat caggtattgt
      601 gcgcgactcc ctcagaaacc gctccagatc ttcacgctta accagatagg ttcgccctgg
      661 cttaaatgct ggcaagcgct tcgagcgtat ccatgagcgg atggtgtcca tcgggacctt
      721 gcgaccgagt acttcataaa tatcctcgac ggtcagccag ctgttgtggt ctaccataac
      781 gagcacccca agtgaaataa aattttcgtc attatagaac acgatggcag gcaggtcaat
      841 gtaccccaaa tgctttatgt aaatcgcgta catcgtttta ttcccatgat gtgttataat
      901 tcttatatat cgtatttcac aatgtttttg agagtaaaaa cggtgaaagg aacgagtccc
      961 gatcttcttg cgcaaccgac cgccgccgtg aagcgcgaat acgagccatt cctgcacccg
     1021 ccaggtgata ccgactggtt tcatcatctc aaacgttaat agtggagagt gcgcagcttg
     1081 tgggtagttc cgttcctgcg cacgcctgat ttgtcgaaca cctaaaacga cattgggtga
     1141 agtaagtcgc tcatgctagt ttgagactta agaacaggac acgtgtctaa agggcagggg
     1201 cgacagaagg gactaatact ctcaggttgt ctttgtagcc ctaagaaagt tgaataaatc
     1261 tgtagatttt acttgagcca agagaggaaa cgagagaatg gcgcaaccca gagcattcaa
     1321 tcttcgccag gcagataacc gccctaataa ctatgccgga actttgccgg agattgacag
     1381 gaaacgggca accgccgtac tggtgaggca gtcaaaaacc ggggcggata cggcgcaggc
     1441 ggaaagccgg gaaacgcagc tggggttgca agattatggc aggctgctct atggcgatga
     1501 cgagccggat gtgcggctgt atgatgaagg tgccggagta tccgggcaaa aacgcattga
     1561 tcagcgagag gtactcgacc gcctctataa ggatatgaac agagggatca taggaaccgt
     1621 cgtactatca agggaggaca ggcttttccg caacaaacat atggatcagg tcggcgcttt
     1681 caccaggctt gccgaggaaa agcggattaa agtcatcgtg ccgccgatat cttccgcctc
     1741 cacagaggag agaacccgcg tctatgactt tatcatttac cgggatctgg ttgcctttca
     1801 ggacaagatg cgcgaagcct acagctacat cgaagggcac gtgaaataca tgcacctgtg
     1861 ccagcagaac aaggcggata agggaggcta tgacgggcgc gctttgcctc ccggccttgc
     1921 ggtcaagggg aaaaaacaag accaggaaat cgtcatctat gaaccctggg cgaatgaaat
     1981 gcgcaagctc gcgctcaggg cgcaggcgct taactgggac atgggcaagc tcaaccggga
     2041 agtagctaag aaggctttcc tttttcccga aataccggag gaagataaag aacggtacat
     2101 gttcagaacc accatgcgtc tgatacccgg cgtcggatat aaaccgctcg acccgcaaac
     2161 catgcgagac tggctgacca acgaaatgct gataggatgg tggcagccgg atgcggataa
     2221 gccagatacg attatcgaca atcatcccgc cgtcctagcc tatgccttgt ttgccgaagg
     2281 ctatgccgct ctgaaaggct atacgttgga aggcgaaccc gtggagaatt ggggaggggt
     2341 aacgcgcatc cgaaagacgc gcgaaacgct gcccgatctg ctgtttcacg gcaggcttgg
     2401 cgtcgcgccg ccctcgccgg acagaacggc atttatatcc gtggatgagc accgatccaa
     2461 atggtactat tacggacata gcaggcaggc cgacggcctg gtggtagata aattcctgag
     2521 cattccgggc gcgccgtttg acaacgtcgt catcgagcgc cttcaggcgc tggaaaaagc
     2581 cgactggcag atgaaggata aggtcagggc gacgttggag caggtgtatg accagcaatc
     2641 cgaggatttc gtcagcatcc tccaccagat cgaaggcatc aaagcccaac tggtcaaaaa
     2701 tgcgaaaaaa cgcatgcgaa ccagcgcgga tgaccccatg tacgccatgc tggaggaaga
     2761 ggcgaaggat ttgttggaac gccagcggga gctggaggcc aagaaagaaa agctcggtct
     2821 ggtggacagc ccagaagaga tcgaaacact gcaccgcctg ctcgggaatt tcgaggccgt
     2881 ctggcccaca tttgacctgg tccagcggca acgcgccttt accctgctta tcaaccggat
     2941 agcggtcgaa gtcgtttctc cccattggct acgcctgtcg gttgactggc tcgatgccat
     3001 atcgccgcgc gtcgatgttg cttatatctg gaaagccatg cccacccgtc atggagctta
     3061 caaattaagt gaggaggaaa agcagattgt tagggctcac tatccccgca cgccgcgtct
     3121 ggagatatta aagctcttgc ctgacaggac atggaactcc ataagggatc aggcacaggc
     3181 gatgcaagcc tggagggaag tacccaccag ggacgatttg tttcaggcga tatgctacag
     3241 agatctggtt cccaacctgg atgggcaata cctgttccgg gactatgaaa ccacgctggc
     3301 gtatatcaga gaagcgggca ggaacacgtc ccggttcggt gcgccgcttt atgcgctgtg
     3361 gatactctcg gagcatgtgg aagacctgct cggcctcgtt gagcggcatt tgaagggcga
     3421 aggctgcgcg ccgcccatgc cgcccagccc ggcggtcagc acgaatttgc ccttgagcgt
     3481 accgccatag tgcttgcgtg ccgcctcgcc gaaggtctca tacgtcccct gcacgatgcc
     3541 ctgcgagccg atatagatcc acgatccggc ggtcatctgg ccgtacatgg tgaggccgag
     3601 cgcctccagg cggcgaaatt cgtcccagtt gccccagtgc ggcaccaggt tcgagttggc
     3661 gatcagtacg cgcggcgcgt ctgcatgggt gcggaagatg cccaccggct tccccgattg
     3721 caccagcagc gtctcgttgt cttcgagcga ctgcaacgag cgcacgatcg cgtcgaaggc
     3781 cgcccacgag cgcgccgccc gcccggaacc gccatagacg acgaggtcgt caggccgctc
     3841 cgccacgtcg ggatcgaggt tgttcattat catacgcatg gcggcctctt gctgccaccc
     3901 cttgcaggtg atgtcgctgc cacgcggcgc gcgaacggtg cgcgggcctg ctgtattgtc
     3961 atggggcatg atgtgatagc tcctttgcta gatgtatgct gaacgagttg caatgtttga
     4021 gtatatccca tagagaaggg gaagagaact atcaatgact atggagctgc gctttatatt
     4081 cttcagtttc tttgttgctc aatttcccgc gtcttcagga ggcttttcta ttccttaaag
     4141 ataagccgca ctacctctct tgttttataa tgtattattc tatttcaaat gcaattgctg
     4201 cttgacaacg cctctgttct ccggtaatat actcaacaac atgtctggct ggtcaccttg
     4261 gggagggtgg ccgggcctgt tccaggagtg tatctttccc atgcgccccc ttactcatgc
     4321 atggggcctt ggcgtgtggt ccttccctat tgcttgtgtg cttacgctct gctttgaaat
     4381 catcaatatg tcctattttt agacgaactg ctcatgcaga acgacccggc gaccgacacc
     4441 ctctccgcgc agagtccgct gctggttggt ccactcatgg cgttgacggg cgtctcacag
     4501 aatcagagtc caccgacgct gcacctggcc tccgtgcagg gcaatagcgg gcaggtgacc
     4561 gacagctatc ccttgcaggt gccgcctggc ccgggcggct ttcaaccgca actggcgctc
     4621 acctactcaa gttctggccc caacgagcga cacgcagcca cctcgcctgc cgcagacgag
     4681 ggcgatggct ggtcgctcag cctggggtcg atcagcgagg agacctatgg cagcaccgtg
     4741 tggtactttc tcaacgatgt cgccggggtc ggcgaccgtc tgatctataa tagcagtacc
     4801 ggcctctacg atacccagca cctctcgtat ctcaaaatcc agcaagtcac gagcagcacg
     4861 accaaccagc cctgctttga cgtatgggac aagtcgggga ccttcaacga gatcggctgc
     4921 accagcacct ccctgcaata ctggaccgat agcagcggcc aacacaacta tcagtgggac
     4981 gtcgataaaa tcgtggcgcc caacgaggga cccaacgcca gtgtctaccg tctgatgctc
     5041 gtgacctacg tccaggatag cgcgacgaca aatggttaca ccaccattcg tgacgcagct
     5101 ctgcaggaga tcgtctatgg caccggcacg tcgaccagca cgatttccaa ggttgtcggc
     5161 accgtcgact ttttctatca cggtccaagc ctgctgaccg gaaccagtga ctcaggcagt
     5221 atcttgacgt ggatcacgcc ctacggcacc aattacgcct gcgcgtcgtc cccaccggtt
     5281 tcaaccccgc tgcgctgtga taatccgcag gatgacggca cggagcaagc gcccctggtc
     5341 atgagtaccc tgacgctcga caaggtcgtc agctacgtcg gaacggatgc gggaaccgcc
     5401 ccagttacgc ggaggcgagt tatgccttca gctatggcaa tgaccagccg tttgtgagct
     5461 gcaccgaccc gctcaccctg gcccaggaat attgcgccgg cgagcatctc ctcttgagca
     5521 tcaccccgac cgcctaccag aataacacgg gccatgccct tcacccgttt gtctttggct
     5581 ataccggctt gcgtaccaac acctactatg atagctccga aaaggtaggg actaatcact
     5641 accaggcgca aaatagctgg cagtacctga acttcttcat cgatctcacc accggcatcg
     5701 gcgagagcgt cagctggcag attgcctaca gcaacacaga cggcaccccc gacgacctca
     5761 cgagccaggg aaccgtcgat gggcgctacg atccactgca ctgcgccacc tggaacgatt
     5821 gcaccggcca ttacgctcat cccgacaatc aatcctggag cgagtgggtc gtcacgcaga
     5881 tcagcgcgct cggcaccgat agcagcgcga ccgcgctgac cccggcggcg acgacctacg
     5941 catatgtgct tgccaaaacc ggcacgtata gcggcaaagt ctactgctac ccctcggcca
     6001 acaatgtcga caaagactgc gtgggcgata actggattcc caaggggaat accgattggc
     6061 aggactacta ccatgccgaa taccagggct ttgcccaggt gtggatcgtc agcccttcac
     6121 aggacctgac ccttgaccag taccacagca ctgaaggctg gtacaccccc tggtccgatt
     6181 accagaattt cgagggcggc tccctgtatt tagaggatcg ctacagcggc aacagcctca
     6241 acctcaccac gctgccgctg ctgagcagcc tctataacac ctacgccgac aacgccaacg
     6301 cctgccgcag cgccagcgcc acctacccag cctgcgaaat cgtgctgacc cccactacct
     6361 ccatcgaata taaccagacg cagcacgaga acccggtctc ggtggaacac agctatgcct
     6421 acgacgacta taccgcctcc g
//
LOCUS       DASZAK010000079         3769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_534534, whole genome shotgun sequence.
ACCESSION   DASZAK010000079 DASZAK010000000
VERSION     DASZAK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534534"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VGT44_01240"
     CDS             complement(<1..706)
                     /locus_tag="VGT44_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2579449.1"
                     /translation="MRIAIISDIHGNLIAFDAVLQDLKEQAPVDHLVIAGDLCLNGPS
                     PRQVIERVQALHCPVIQGNTDVEIVTQAPDKGEKKRNTVGWTREQIGQPGIHYLNSLP
                     FSHTIATPGGSTLLIVHANPLNVEDAIFPNASDEELEGWLGGLDTRIGALAFGHLHIA
                     YTRWWRSLLLCDVGSCGIPRDEDHRASYGILTWNEKGWEAEIHRVTYDVEAVIRQLRE
                     CGMPNPDKRIKVLVEAR"
     gene            815..1312
                     /locus_tag="VGT44_01245"
     CDS             815..1312
                     /locus_tag="VGT44_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2579450.1"
                     /translation="MNSQHRIGVISDTHIPHFRRLPDAIWQHFDGVELILHAGDLSVL
                     SVIAQLETIAPVEAVRGNIEEDDVILKLPIKREIMVGHCRIGIVHILGDAQSHAKMAR
                     QEFPLARCVVFGHSHIPWNEDRDGQLLFNPGSATDRRRQPQCSIGLLYVDDEARTVWG
                     EIIYI"
     gene            complement(1353..2102)
                     /locus_tag="VGT44_01250"
     CDS             complement(1353..2102)
                     /locus_tag="VGT44_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579451.1"
                     /translation="MAIHFARRLITVSGAMLGAYIFFVRPWQRRWGATDEEVQRALPG
                     DELLPHPDVKLTRAITIRAKPTDIWPWLVQIGQGRGGYYSYDWLENLAGLKMKSAAGI
                     NPAWQQLKVGDIIPAEPGGKGFKVLAIEPARALVIGSIEMVDEGVFEGFKQMFPAFTW
                     AFILEEIDSEHTRLISRFCGQNEPSQAATLLNKSLGFIFEPAEFLMTRKMLLGIKQRA
                     EQIGVPAIEPVTTEPKEKPHKNGKVASATHI"
     gene            complement(2262..3542)
                     /gene="serS"
                     /locus_tag="VGT44_01255"
     CDS             complement(2262..3542)
                     /gene="serS"
                     /locus_tag="VGT44_01255"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV2579452.1"
                     /translation="MLDLTFIRNHPDIVKEAARVKNSSFDVDHFLEIDRQALALQRQV
                     EEARAEQNRVSKRIQQAGKEKDLRDALIAEGKQLAEHIKALEPQLRALKEERQQLLYY
                     VPNIPDPSAPIGKDESDNVPFKYWGEKPTFDFEPRDHYALMQNLGVVDIERAVKIAGA
                     RSYALKGDAARMELALMNFAMDRIARKGFTPVIVPAMARDFCFLGSGQFPKGRDQVYA
                     VEDEDVFLVGTAEVSITGMYKDEILRAEDLPMTMIGYCPCFRKEAGTYGKDTRGVFRI
                     HQFNKIEQYVICKADHAESVRWHEQLLANSEELVQALELPYRVVNVCTGDMGDGKVGM
                     YDLECWLPSEGRYRETHSCSYFHDWQARRVNIRYRDEDGKVRFVHTLNNTAIASPRIL
                     IPIFEVHQQADGSVRIPEALRPYMGGQEYIRPRE"
BASE COUNT          726 a         1076 c         1096 g          871 t
ORIGIN      
        1 agcgagcttc caccagcacc ttgatgcgtt tgtccgggtt gggcattccg cactctcgta
       61 gctggcgtat caccgcttcc acatcgtagg tgacgcgatg tatttcagcc tcccaaccct
      121 tttcgttcca ggtcaggatg ccgtaagagg cgcggtgatc ctcgtcgcgt ggaatgccgc
      181 aactgcccac atcgcaaagc agcaggctgc gccaccagcg agtataggca atatgcagat
      241 ggccgaatgc cagcgcaccg atacgtgtat ccagaccgcc aagccagcct tccagttctt
      301 cgtcggaggc gttggggaaa atggcgtctt ctacgttcaa cgggttggcg tgaacgatca
      361 gcagggtact gccgcccggg gtggcgatcg tatgcgagaa tggcagcgag ttgaggtagt
      421 gaatacccgg ctgaccgatc tgctcgcgcg tccacccgac ggtattgcgt ttcttttcgc
      481 ctttgtccgg ggcctgtgtc acaatctcta catcagtgtt cccctggata accgggcaat
      541 gcagcgcctg tacgcgctcg atcacctggc gagggctggg gccattcagg cacaggtcgc
      601 cggcgatgac aaggtggtcg accggcgctt gctctttcag gtcttgcaaa acagcgtcga
      661 atgcgataag gttgccgtga atgtctgaaa tgatggcaat gcgcatgttc tgtccttttc
      721 gatcgttcca tgtcaatcaa tgcagacgtt agcggtcgtt cgcacgtgtg agaccatcca
      781 tatcatatcg caaaatggga gtgttagcca cattatgaac tcacaacacc gtatcggagt
      841 gatctccgac acgcacatac cgcacttcag gcggctgccc gacgccatct ggcagcattt
      901 cgatggagtc gagttgattc tccatgcggg cgatctttca gtgctgtcgg tcatcgctca
      961 actggagacg atcgcgccgg tcgaggcagt gcggggcaac atcgaggaag acgatgtgat
     1021 actgaaactg ccgatcaagc gcgaaatcat ggtggggcat tgccgcattg gcatcgtgca
     1081 tatcctgggc gatgcgcaga gccacgcaaa gatggcgcgc caggaatttc ccctggcgcg
     1141 atgcgttgtc ttcggccaca gccatattcc ctggaacgag gatcgcgatg ggcaactgct
     1201 cttcaatccg ggcagcgcca cggaccgccg aaggcagcca cagtgtagca ttgggctgct
     1261 gtacgtggat gacgaggcgc gaacagtgtg gggggagatc atttacatct aattgtctgc
     1321 ctccccaccg taagccatta ctggctctct agctatatat gcgtggcgct ggccactttt
     1381 ccgttcttat gtggcttctc cttcggttct gttgtgaccg gctcgatagc agggacaccg
     1441 atctgctctg cgcgctgctt gatgcctagc agcatcttac gtgtcatcaa gaattcggct
     1501 ggttcaaaaa tgaagcccag tgatttgttt aacaacgtgg ctgcctgaga cggctcgttc
     1561 tggccgcaga agcgggagat caggcgtgta tgctcgctgt cgatctcttc gaggatgaag
     1621 gcccaggtaa atgccgggaa catctgcttg aagccctcaa agacaccctc atcgaccatc
     1681 tcgatgctgc caattaccag ggcgcgtgct ggttcgatgg cgagtacttt aaagcccttg
     1741 ccgcctggtt cggctggaat gatgtctcca actttgagtt gttgccaggc gggattgatg
     1801 cccgcggcgc tcttcatctt gaggccggcc aggttctcga gccagtcgta gctgtagtaa
     1861 ccgcctctcc cctgcccaat ctgcacaagc cagggccaga tatcggtggg ttttgctcga
     1921 atcgtaatag cccgcgtgag cttcacgtca ggatggggta gtagttcatc accgggcaat
     1981 gcgcgctgaa cctcttcgtc ggtggcgccc cacctgcgct gccaggggcg aacgaaaaag
     2041 atgtaggcgc ctagcattgc cccgcttacc gtgatcaatc ttctggcgaa gtgtattgcc
     2101 ataaatgtgt tcctccttgt gtgcgtaaag tcatactcca gtcgtcagag ctgactacct
     2161 tccagcttgg atgttactac gcgggtttca cgtacccatc acgaatacgg tagagcgccg
     2221 cacaaggagg tcacaatttg tattgatggg tcttatcgcg cttattctcg tggtcggata
     2281 tactcctgtc cgcccatata ggggcgcagt gcctccggga tgcgcacgct gccgtcggcc
     2341 tgctggtgta cctcgaagat gggaatcaag atgcgcggcg aggcgatggc ggtgttgttc
     2401 agcgtatgca caaacctgac cttaccgtct tcgtcgcgat agcggatgtt gacgcggcgc
     2461 gcctgccagt cgtggaagta cgaacacgag tgggtctcgc ggtagcggcc ttcgctgggc
     2521 agccagcact ccaggtcgta catgcccact ttgccatcac ccatatcgcc ggtgcagacg
     2581 ttcacgacgc gatagggcag ttctagcgcc tgcaccagtt cttcggagtt ggccagcaac
     2641 tgctcgtgcc agcggaccga ctcggcgtgg tcggccttgc agatcacgta ctgctcgatt
     2701 ttgttgaact ggtggatgcg gaagacgccg cgcgtatcct tgccataggt gccggcctct
     2761 ttacggaagc aggggcaata gccgatcatc gtcatgggca ggtcttcggc gcgcaggatt
     2821 tcatccttgt acatgccggt aatcgagacc tcggcggttc ccaccaggaa gacatcctcg
     2881 tcctcgacgg cgtagacctg gtcgcgcccc ttggggaatt gcccgctgcc gaggaagcag
     2941 aaatcgcgcg ccatagccgg tacaatcacc ggcgtaaagc ctttgcgggc gatgcggtcc
     3001 atggcgaagt tcatcagggc cagctccata cgagcggcgt cgcctttgag ggcgtagctg
     3061 cgcgccccgg cgatcttcac ggcgcgctcg atatccacca cgcccaggtt ctgcatcagc
     3121 gcgtagtgat cgcgcggctc gaagtcgaag gtcggcttct cgccccaata tttgaacggc
     3181 acgttgtcgc tttcatcttt gccgatgggc gcgctcggat cgggaatgtt ggggacgtag
     3241 tagagcaact gctggcgttc ctctttcagc gcgcgcagct gcggctccag cgccttgata
     3301 tgctcggcga gttgtttgcc ctcggcgatc agcgcgtcgc gcaggtcctt ctccttgcca
     3361 gcctgctgga tgcgctttga gacgcggttc tgctcggcgc gggcttcctc gacctgccgc
     3421 tgcaaggcca gcgcctgccg gtcgatctcc aggaagtgat cgacatcgaa tgaactattt
     3481 ttgacgcgcg ctgcctcttt gacgatgtcc gggtgattgc gaatgaacgt aagatcaagc
     3541 atgatgtgtg ctactccttc tttcttgcca actactttcg ataattgtgc gaaagatgtg
     3601 taggggcgca attcattgcg ccctcgttcc cggttgaagt ggtctggcaa gcgggcgcaa
     3661 ttcattgcgc ccctacacat ctttcgcaca atcatcgaaa agtagttgga aggagtacaa
     3721 cacatcatgc ttgatctcac gttcatccgc actcacccgg acatcgtca
//
LOCUS       DASZAK010000080         6126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_534553, whole genome shotgun sequence.
ACCESSION   DASZAK010000080 DASZAK010000000
VERSION     DASZAK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534553"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VGT44_01260"
     CDS             <1..579
                     /locus_tag="VGT44_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579453.1"
                     /translation="QASYLDQMQTAMNTAVHPGGNWSWRSQAIGLIDSWHNGYPSQSI
                     NNLYGLFAFWAQQFNVNLNGGALGKHLWVVEGTGCFKGCGINAYDPYQVAVSHILTLI
                     SDVQTTMQYGVPFFFFNYRDFIQSGVYWPIGLLNVGGGAKPIRQDLSMGARALAMGCA
                     GGQVNVVNQVQLIATMYRGCSLPGNYLGVLTS"
     gene            589..2031
                     /locus_tag="VGT44_01265"
     CDS             589..2031
                     /locus_tag="VGT44_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV2579454.1"
                     /translation="MPDQSNQSDQSSLFGADMPLSTPRKAASADDALSPAAQAAAQFE
                     PLAARMRPRNIDEIIGQEHLLAPGRILRRSIEEDRIPSMILWGPPGSGKTTLADVIAR
                     QSHARFARLSAVSAGVADLRKVVEDAAKLRQFSKQRTIMFIDEIHRFNKAQQDAVLPH
                     VERGVVTLIGATTENPSFEVNAALLSRCRVFTLKALTEEQVITILRRALQDTERGLGK
                     LAIQIDEDALQQIAVFANGDARTALNVLELAAQGDANSSDATVRIALASIEDVMQHRA
                     LLYDKSGDQHFDTISALHKSLRGSDPDAALYWLARMIEAGEDPLYIVRRLIRFASEDV
                     GMADPQALVVCVAAQQAVHFVGLPEANLALAQAVVHLATAPKSNALTEAYSRVQEDVQ
                     QTRNDPVPLQIRNAPTQLMKDLDYGKDYKYAHDYYKDMHIDDPERPPAISLQEYLPES
                     LKGRRYYEPGQQGKEASIKKWLEKRRGKES"
     gene            complement(2034..2309)
                     /locus_tag="VGT44_01270"
     CDS             complement(2034..2309)
                     /locus_tag="VGT44_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579455.1"
                     /translation="MPEQRQEWVLCKPCHSALLVEMRRSSLRSPTRLRIAMGLVAAER
                     SPNAYIVSTPVREQQAFQKEFAWFARLLILFTLFHVVLFVILLAVPK"
     gene            complement(2594..3301)
                     /locus_tag="VGT44_01275"
     CDS             complement(2594..3301)
                     /locus_tag="VGT44_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579456.1"
                     /translation="MPKFAVYYIPQTDDPFYQVGTQILGYDVRSRQSVAASSELEEVL
                     GAGRQSWTTVCRPYGFHLTICEALDGHWATIPRVEQELADLVKCFDPSRPFVLERRDD
                     QPVGIWGEEASKSLVLLYKPNTSLHLLHTLLVARLTLLGTGSDFLKRYLTHSQQERPL
                     YEVQQLRLFSSPKVLENWYPHFTLLNPYTGEEALAMASRLAQLFQTYRHVVVPTICLL
                     IQEHHEANWQIYQEFRR"
     gene            complement(3353..3601)
                     /locus_tag="VGT44_01280"
                     /pseudo
     CDS             complement(3353..3601)
                     /locus_tag="VGT44_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917309.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3641..5227)
                     /locus_tag="VGT44_01285"
     CDS             complement(3641..5227)
                     /locus_tag="VGT44_01285"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEV2579457.1"
                     /translation="MSTLLRLPTATRVATTPLQPIHADRLIIATNRGPVEYFMSQQQV
                     LKPRRGAGGVVTALVGAAKNIDVSWVAMAMTEGDRIAIKERQQDGVMKSPLRDQNMLL
                     RYVAIPKTAYRKHYEQISNQLLWFVQHYMYDSNGEEQSNRKIQDAWNNGYMVANRAIA
                     DAVNAEIERGEGTPVVMLHDYHLYLAPATIREVHPTIIMQQFIHIPWPDIRCWQLLPG
                     NISEAIFNGLVGNDIIGFQTERDAHNFMEGARTILDGSVVDFESGAIWWKGHHTQVRA
                     YPISISVTDEKRQAQSLAGKRAVEPLRPLLEKQTIMRVDRIEPTKNILRGFQAYALML
                     EQHPELRGAVNFLAFLVPSRQTLSKYKRLGSDVEKLVNEINERFGTMDWKPITAFYEN
                     DRVQALAAMRFYDVLLVNPIIDGMNLVAKEGPVVNRQNGVLVLSRTAGAFQQLGKAAI
                     PVTSTDVAETANALYQALTMPRDERRAMSQQARHAVEDHDLNEWLQCQFDDINALLDR
                     LSARVSAHEAIYSVPVENAG"
     gene            5586..>6126
                     /gene="rph"
                     /locus_tag="VGT44_01290"
     CDS             5586..>6126
                     /gene="rph"
                     /locus_tag="VGT44_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR01966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEV2579458.1"
                     /translation="MTRIDLRQPTQLRPLRITVDYLDYAEGSVLIEAGKTRVLCAASV
                     EERVPPFLEGKGTGWVTAEYNMLPRSTHTRTSRERDGRVSGRTQEIQRLIGRSLRAVV
                     DMQKLGPRTITLDCDVIQADGGTRTAAITGAYVALHRACTGLAQRGVLAEHPLRTAVA
                     AISVGVVGGELLLDLCYQED"
BASE COUNT         1309 a         1804 c         1729 g         1284 t
ORIGIN      
        1 caggccagct acctcgacca gatgcaaacc gccatgaata ccgccgtcca ccccggcggc
       61 aactggtcct ggcgctcgca ggctatcggc ctgatcgaca gctggcacaa cggctacccc
      121 agccagagta tcaacaacct ctacggcctg ttcgccttct gggcgcagca gttcaacgtc
      181 aatttgaacg gcggcgcgtt gggcaagcac ctctgggtgg tcgagggaac cggctgcttc
      241 aagggctgcg gcatcaatgc ctacgatccc taccaggtgg ccgtctcgca tatcctgacg
      301 ctgattagcg acgtgcagac aactatgcaa tacggtgtgc cgttcttctt cttcaactat
      361 cgcgatttca ttcagagcgg cgtctactgg cccatcggtc tgctgaatgt cggcggtggc
      421 gccaagccca tccgccagga cctctcgatg ggcgcgcgcg cgctggcgat gggctgcgcc
      481 ggcggccagg tgaacgtggt caaccaggtg caattgatag ccacgatgta ccggggctgt
      541 tccttgcccg gcaactacct gggtgtgctg acgagctgag gaaaaatcat gccggaccag
      601 tcaaaccaat cggatcagtc gtcgttgttt ggcgctgata tgcccctttc aacgccccgc
      661 aaggctgcat cggctgatga tgctctgagt ccggctgccc aggccgccgc gcagttcgag
      721 ccacttgccg ccagaatgcg tcctcgcaac atcgatgaaa ttatcgggca ggaacacttg
      781 ctggcccccg gtcgcatctt gcgccgcagt atcgaagaag accgcatccc atccatgatc
      841 ctgtgggggc cgcccggcag cggcaaaacc acgctggcgg acgtgatcgc gcgccaatca
      901 cacgcgcgtt tcgcccggct cagcgcggtc tccgccgggg tcgccgacct gcgtaaagtg
      961 gtagaggacg ctgcaaaatt gcgacagttc tccaaacagc gcaccatcat gtttatcgat
     1021 gaaatccatc gcttcaacaa agcccaacaa gatgcagtct tgccccacgt cgagcgcggc
     1081 gtcgtcacct tgatcggcgc gaccaccgaa aaccccagct tcgaggtcaa cgccgcgctg
     1141 ctctcacgct gccgcgtctt caccctcaaa gcgctgacgg aagagcaggt catcaccatc
     1201 ctgcggcgcg ccctgcaaga taccgaacgc ggattgggaa agctcgccat ccagatcgac
     1261 gaggatgcgc tgcaacagat cgcggtcttc gccaacggcg acgcgcgcac tgccctgaac
     1321 gtactggaac tggccgcgca aggggacgcg aatagcagtg atgcgactgt acgcattgcc
     1381 ctcgcttcta tcgaagatgt tatgcagcat cgcgccttgc tctacgacaa gagcggcgac
     1441 cagcacttcg acacgatttc cgcgctgcac aagtcgctgc gcggctccga ccctgacgcg
     1501 gccctctatt ggctggcgcg catgatcgaa gccggggaag acccactcta catcgtgcgc
     1561 cgcctgatcc gtttcgcctc ggaagacgtg ggcatggccg atccgcaagc gctggtggtc
     1621 tgcgtcgccg cccagcaagc tgtgcatttc gtgggtctgc ccgaagccaa cctcgccctg
     1681 gcacaggccg tcgtccacct ggcgaccgcc cccaaaagca acgccctcac cgaagcctac
     1741 agccgcgttc aggaagatgt gcagcagacg cgcaacgacc ccgttcccct acagattcgc
     1801 aacgccccca cgcagttgat gaaggacctc gactacggca aagattacaa atacgcccac
     1861 gactactaca aagatatgca tattgatgat cccgaacgac cacccgccat ctcgctgcaa
     1921 gagtatctgc cggagagcct caaagggaga cgctactacg agccaggaca gcagggcaaa
     1981 gaagccagca tcaagaagtg gctggagaag cgacgaggta aggagtcgta aacttacttc
     2041 ggtaccgcta acaaaatgac aaacagaacc acatgaaaga gggtaaacag gatcagcagc
     2101 cgtgcaaacc aggcgaattc tttctggaat gcctgttgtt cccgaacagg agtacttaca
     2161 atataggcat tgggcgagcg ttcggcagcc acaagcccca tagcaatgcg caggcgcgta
     2221 ggcgaacgta aggaggatcg gcgcatttca acaagaagcg cactgtgaca tggtttgcag
     2281 agaacccact cctggcgttg ctcaggcact tcaaccggct ctttgagtac aaaggcgata
     2341 agccagataa aacccgtaca gatcgaacag cgtataggga tgagccagcg tattattctc
     2401 tgaccagttt cgcgcccggt tgcggctatt ccgtcatcac ctggtgtcat atgtttgtac
     2461 gctcgctgtt ttctccaggc gcattgccgg gtttaagcag gcaagagaac tcttctttgt
     2521 tcgctaacca atgcaattat tgtaccataa tccggttctt ccgcaggctc gctgatgctg
     2581 aggattggtc aagtcatcga cgaaactcct ggtatatctg ccagttggct tcatggtgct
     2641 cctgaatgag cagacaaatc gttggcacca cgacgtgcct gtatgtttgg aagagctggg
     2701 caaggcgtga ggccatcgcc agtgcctctt ctccggtata ggggttgagc aaggtgaagt
     2761 gaggatacca gttctccaac accttggggc tggaaaacaa ccggagctgc tgcacctcat
     2821 aaagcggtcg ttcctgctgt gagtgtgtca ggtagcgctt cagaaagtct gaaccggttc
     2881 caagcagagt cagccgtgcc acgagaaggg tatggagcag gtgcagagac gtgttgggct
     2941 tatagagcag caccaagctt ttgcttgcct cttcccccca gatgcctact ggctggtcat
     3001 ctctgcgctc aagtacaaag ggacggctgg gatcaaagca tttgaccaga tcagccagtt
     3061 cctgctccac acgagggatg gttgcccaat ggccatcgag tgcctcgcag atggtcaggt
     3121 gaaatccata ggggcggcag accgttgtcc agctttgtct gcctgccccc agaacctcct
     3181 ccagctcaga ggacgcagcg acgctctggc gggaccgcac atcgtagccc aggatctggg
     3241 ttcctacctg atagaaagga tcatctgttt gagggatgta gtatacggca aattttggca
     3301 tggtgggctt ctccctgctc ccgtgctagg ctgccaggag caggctggca gcctagctct
     3361 gctgctcatg cttcacgtct tctcctggtg tgacatgaag cacacgcgct cgcaggagag
     3421 gaagcttggc acggccatac atgctccttt tcagcaattt gagacgattg acatgaccct
     3481 ccgttggccc attgctccaa gggagcgtca atccggttag cacagccgct ttgtcttggt
     3541 ggatacccct gacaaatgac ttgaactcag gcagatggct ctcttcagct tctcgaagcc
     3601 aggtgttaag gagcagaacg tctctcatct cagggcaacc ttaccccgcg ttctccacag
     3661 gcacactgta gatcgcctcg tgcgcggaga cgcgcgcgga gagacggtcg agcagggcgt
     3721 tgatatcgtc aaactggcac tggagccatt cgttgaggtc atggtcttcg acggcatggc
     3781 gcgcctgctg cgacatagcg cgccgctcgt cgcgcggcat tgtcagggcc tggtaaagcg
     3841 cattggcggt ttcggcgaca tcggtggaag tgaccggaat agccgccttg cccaattgtt
     3901 ggaacgcgcc cgccgtgcga gatagcacca gtacgccatt ttgccggttg accaccggcc
     3961 cctctttcgc aaccaggttc ataccgtcga tgatagggtt gactagcagc acatcgtaga
     4021 agcgcatggc cgccagcgcc tgcacacggt cgttttcgta gaaagcggta attggcttcc
     4081 agtccattgt gccaaagcgt tcgttgatct cgttgactag cttctcgaca tcggagccga
     4141 gccgcttgta ctttgacagc gtctggcgag acggcacgag aaacgccaga aaattcacgg
     4201 ccccgcgcag ctccgggtgc tgctccagca tcagggcata tgcctggaaa ccccgcagga
     4261 tattctttgt cggttcgata cggtcaacgc gcatgatcgt ctgcttttcc agcagaggcc
     4321 tgagtggctc tactgcgcgt ttaccggcca gactctgcgc ctggcgtttt tcatctgtta
     4381 ccgagatgga gataggatag gcgcgcacct gcgtatggtg tcccttccac cagatcgctc
     4441 cgctctcaaa atcgacgacg gagccatcca ggatcgtgcg cgcgccttcc atgaagttgt
     4501 gcgcgtcgcg ttcggtctgg aaaccgatga tgtcgttgcc gaccagcccg ttgaaaattg
     4561 cttctgagat attgccaggc aagagctgcc agcagcgaat atcaggccag ggaatgtgaa
     4621 tgaactgctg catgatgata gtcgggtgaa cctcgcgaat cgtggccgga gcgaggtaga
     4681 ggtgataatc gtgcagcatc acgacgggcg tcccttcgcc acgttcaatc tcggcattca
     4741 ccgcgtcggc gatagcgcga ttggcgacca tatagccgtt gttccaggca tcctggatct
     4801 tgcggttgct ctgttcttcc ccattcgaat catacatata atgctgcaca aaccagagca
     4861 gttgattact gatttgctcg tagtgcttgc gataggcagt cttgggaatg gccacgtaac
     4921 gcagtaacat gttttgatcg cgcaggggcg acttcatgac gccatcctgc tgcctttcct
     4981 tgatggcgat acgatcacct tcggtcatcg ccattgccac ccagctgaca tcaatatttt
     5041 tggccgcgcc taccagggcg gtgacgacgc cgcctgcgcc tctacgaggt ttcaagacct
     5101 gctgctggct catgaaatac tcgaccgggc cgcgattagt ggcaataatc agacgatctg
     5161 cgtgaatggg ctgtagcgga gttgtcgcta ctctcgttgc tgttggcaaa cgaagtaaag
     5221 tggacatctt atccgtcctt tctgacaaac caccgctcgt cgcgcgcgtg gtcgttgatt
     5281 accgtgttac acccgccagg ggttgttttt gcatgaatgg gttccaacgg cgcgcgctac
     5341 gcttttgcgc gccgacctca ccggcatctc gacgctttct acaaagatac acggttatgc
     5401 aggcttaaac agcgaaggtt atagtagtac atcgtgatat atcttaattg atcctgtgta
     5461 attgtaccac acggcggata aaatatatgt aaatgtttgc cagcttgctc ttacttttgg
     5521 gctatttgcc cctggtgaga gcgcgcccgt cgtacactta cataaagaaa ggtcaacctt
     5581 tatctatgac acgaatcgac ttacgtcaac cgacgcaact acgtccgctg cgcatcaccg
     5641 tcgattacct ggactacgcc gaggggtctg ttctgatcga ggcgggcaag acgcgcgtcc
     5701 tgtgcgcggc cagcgtcgag gagcgagtgc cgcccttcct ggaaggcaag ggaacgggat
     5761 gggtcacggc ggaatataat atgctgccgc gctcgacgca tacccgcact tcgcgtgagc
     5821 gcgatgggcg cgtgagcgga cgcacgcagg aaatccagcg cctgattggt cgctccctgc
     5881 gcgcggtggt ggatatgcag aagctcggcc cgcgcaccat cacacttgac tgcgatgtca
     5941 tccaggccga tggtggtacg cgcacagccg ccatcaccgg cgcgtatgtc gcgctccacc
     6001 gcgcctgtac agggctggcg cagcgcggtg tactggcaga acacccacta cgcacagcgg
     6061 tcgcagcaat cagcgtcggc gtcgtgggcg gagaactcct gcttgacctg tgctaccagg
     6121 aagatt
//
LOCUS       DASZAK010000081         1817 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_534575, whole genome shotgun sequence.
ACCESSION   DASZAK010000081 DASZAK010000000
VERSION     DASZAK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534575"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VGT44_01295"
     CDS             complement(<1..343)
                     /locus_tag="VGT44_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579459.1"
                     /translation="MLTDDYELTREDIQGLSNRDALAAFFARLGYDTNLRLAQNAAAM
                     GFGSDQLKSTITHIERLASQDGGLLEVYLVELKSVTVAITRALTQAFRNLSVQHPLLV
                     LTDDYQRLDFVL"
     gene            complement(827..>1817)
                     /locus_tag="VGT44_01300"
     CDS             complement(827..>1817)
                     /locus_tag="VGT44_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579460.1"
                     /translation="KNNVSNLAILLLQAPLVALLLILLVRFEIGSGIFDPNHLVQCRT
                     QILTSSGPLALPGIANQADLVNCQQVVNFLNANPTGVQYAQVRGGTNQALQDFIVPAQ
                     SAEAQLVIFLVAFFAVLFGCINGTREIVKEAAIYQRERTVSLGILPYLFSKITILGAL
                     ALVQAASILFIVEIFEPFNQSVFLPVLVEVYITLALAGVAGVMMGLLVSAIAPNDDTA
                     NSLLPIIIIPQVIFAGSIIPLKDWLTQAVAALFPTRWALAALGSSLGLHSDKIDGGKL
                     FGDDFTYHSTLFSTYSQADATQRVILAWAALGATILILTCVIGILLKRKDVRR"
BASE COUNT          387 a          459 c          599 g          372 t
ORIGIN      
        1 tcagcacgaa gtcgaggcgc tggtagtcgt cggtgaggac gagcagggga tgctgcacgc
       61 tcaggttgcg aaacgcctgc gtcagcgcgc gggtgatggc gacggtgacg gatttcagtt
      121 cgaccaggta gacttcgagc aggccgccgt cctgactggc gaggcgttcg atatgcgtga
      181 tagtcgattt gagctggtcg ctgccaaagc ccatggcggc ggcgttctgc gccagtcgca
      241 gattggtgtc gtagcccagc ctggcgaaaa atgcggccag ggcatcacgg ttggacagac
      301 cctggatatc ctcgcgggtg agttcgtagt cgtcagtcag cacgtaccag cctcaacctt
      361 ccagtcggta tgtattggga gttgctcgat gcagttaccc tgactctcgt agggcaagac
      421 tagcatgagt ggctggggtt gtcacgcgtg ttggcaaacg agcgccagcc ctcaggaaga
      481 gccggttatg cacattgaca aattaatgcg gacatcgcag agccagcccc agggcgaccg
      541 caagggtctc caccccgctc cgctccaccc ccgcccttac aatgactacg aggccgctcg
      601 acacgccgat ccgtcgttct ttgtcagggc gggggtggag tggcgtgggg tggagaccct
      661 tgcggtcgcc ctcgccttca ctgctccggt ggccggggta gtttgtcaat gtgcattatc
      721 ggctagtcag gcgtttcgga acgacgagga ttcgggccga taaatcggct gcgagcgcga
      781 taaatcggct cctacggttt tgttgttaag gatgatgtac tacgggttat cggcgcacgt
      841 ctttgcgttt cagaaggatg ccgatgacgc aggtgagaat caggatggtt gcgccgagag
      901 ctgcccaggc caggatgacc cgttgtgtgg cgtcggcctg gctgtaggtg gagaagaggg
      961 tgctgtgata ggtgaaatcg tcaccgaaca gttttcctcc atcgatcttg tcgctatgca
     1021 gaccgagaga cgagcctaac gcggctaagg cccagcgtgt tgggaagagc gccgcgaccg
     1081 cctgcgtgag ccagtccttg agggggatga tggaaccggc gaaaatgacc tgtggaatga
     1141 tgataatggg aagcaggcta ttcgctgtat cgtcgttggg ggcgatagct gagaccaata
     1201 gccccatcat gacccctgcc acgccggcca gggccagggt gatgtacacc tccacaagca
     1261 cgggtaagaa aacgctctgg ttgaatggtt caaatatttc gacgatgaac agaatagagg
     1321 cggcttgtac gagggccagc gcgcccaata tagtaatttt agagaacagg taagggagga
     1381 ttcccaggga gacggtgcgt tctcgttgat aaatggcggc ttctttgaca atctcgcgtg
     1441 tgccgttgat gcagccaaag aggactgcga aaaaagcgac gaggaagatg acgagctgag
     1501 cctcagcgct ctgggccgga acgatgaaat cctgcaacgc ctggtttgtg ccgcccctca
     1561 cctgcgcata ctgcacaccg gtgggattgg cattgaggaa gttgacgact tgctggcagt
     1621 tgaccaggtc tgcctggttt gcaatgcctg gaagcgctag cgggccggag gatgtgagga
     1681 tctgcgttct acactggaca agatggttcg ggtcaaagat gcctgagccg atctcgaagc
     1741 gaaccagcag gatgagcagc agggcgacga gcggcgcctg caacaggaga atggcgagat
     1801 tactgacatt gttcttg
//
LOCUS       DASZAK010000082         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_357179, whole genome shotgun sequence.
ACCESSION   DASZAK010000082 DASZAK010000000
VERSION     DASZAK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357179"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VGT44_01305"
     CDS             complement(<1..224)
                     /locus_tag="VGT44_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579461.1"
                     /translation="MNYEPSLPMQVEMFAASMRASYTDVGAFLEALAVKFEGALPTHT
                     KVSRHSGLFSREHSVKEIVVMLGDYHYRIG"
     gene            533..1483
                     /locus_tag="VGT44_01310"
     CDS             533..1483
                     /locus_tag="VGT44_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579462.1"
                     /translation="MIPLSSGGEVASFLDVNQFQPGRTQFACGFFACAIVKGMMPVGH
                     TPVQTAQEMVAEAEGWYAQYDGNNSSANLDGMTQQQLYDLQAQIGLHGLATSQEPEML
                     RAWLRLGYPLIVAGVESSFHDVALDGAVPYPWHPQGNHIIVLTGVTPDGNFLARDPAN
                     VTDLYNPDSLRPGPRAYDAAQMQLVSATVIVPPWLPVPPADFDPRRKQSTPAIPIGWK
                     DDGITLTAPNGHSVVRGFREYILTHAWHPENVPLQEEQGRDPLEESNPSLGGGTQQIF
                     NWTMLEWTQARGVFVAWIGQELLRLRADLQTRNNGGNTIP"
     gene            1717..2766
                     /locus_tag="VGT44_01315"
     CDS             1717..2766
                     /locus_tag="VGT44_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV2579463.1"
                     /translation="MSHLATPESDAPLQADVVLEGGGVRGIGHVGALCIAEEQGYQWV
                     NIAGTSAGAFVAAMLAAGYSGQEMYTIMRDEVDFTLFARDRGIDGLAPVEALHLLRQG
                     GLHNGSYIESFIRGKIQAKGVTTFGDLVLREHEPKHSSVRYRLTVIASDISSGQMLRL
                     PQDMSQFGFDPDRIEIAKAVRMSASIPFFFIPVAQKDNEGRNCLIVDGGLLSNFPVYL
                     FDVQTQPKRPTFGLRLVDSPPTPSCPWPFNPTNNMLEITHALLQTMLTAHDRLYIDDH
                     SYVRTIAIPVNGISDTKFNLSKQEANTLYQNGRHAAQDFFSTWDFEAYKAAFRGDQPQ
                     QSRRERLTASMKQAK"
BASE COUNT          616 a          859 c          795 g          553 t
ORIGIN      
        1 ttgccgatgc gataatggta gtcgcccagc atcaccacaa tctccttaac agaatgctct
       61 cgcgagaaca gcccgctgtg gcgactgacc ttcgtatgcg taggaagcgc gccctcgaac
      121 ttgacggcca gcgcctccaa aaacgctccg acatccgtat agctggcgcg catcgaagcc
      181 gcgaacattt ctacctgcat gggcagcgaa ggctcgtagt tcatgtcgtg ttccttcaat
      241 caagatttgt cttccgccat aattcatgag catagcccgt tgccgtgtat tatagcatag
      301 gggcaatgaa ctcagaatcc ttcagggcta aggcgcagtc cagttgggca tggcgcaccc
      361 ttgcggtcgc catgtaacca gtcacaccca ccatgccata catgactaga caaaaagtca
      421 atttcaatgt atacttcctc taagcaagcg tgctgtattt acagcgacgt tcctcgttct
      481 tcctcgttta tcgcaatggt aaacacccat cccctcacgg aggcatttcc ctatgattcc
      541 cctttcctca ggcggcgaag tcgccagctt tctcgatgtc aaccagtttc agccggggag
      601 gacgcagttt gcctgcggct tcttcgcctg cgctatcgtc aaaggcatga tgcctgtcgg
      661 ccatacgccg gtgcagacgg cgcaggagat ggtcgcggag gcggagggct ggtacgcgca
      721 gtatgatggt aacaattcca gcgcgaattt ggatggcatg acccagcaac aactgtatga
      781 cttgcaagcg caaatcggct tgcatggcct ggcgaccagc caggagccgg agatgttgcg
      841 cgcctggctg cggctcggct acccgctgat cgtggcgggc gtggagagca gctttcacga
      901 cgtggcgctt gacggcgctg tgccgtatcc gtggcatcca cagggcaacc acatcatcgt
      961 attgacgggg gtcacgcctg acggcaactt cctggcgcgc gacccggcca acgtcaccga
     1021 cctgtacaac cccgacagcc tgcgaccggg accgcgtgcc tacgatgccg cccagatgca
     1081 actggtgagc gctactgtga tagtcccacc ctggctgccg gtgccaccag cagacttcga
     1141 ccctcgcagg aagcagtcca cgcctgctat tcctattggc tggaaagacg acggcattac
     1201 gctgaccgcg ccgaatggtc acagcgtcgt gcgcggcttt cgcgaatata tcctgaccca
     1261 tgcctggcat cccgaaaatg tgccattgca ggaagagcag gggcgcgacc cgctcgaaga
     1321 atccaaccca tcgctcggcg ggggaacgca gcagatcttc aactggacga tgctcgaatg
     1381 gacgcaggcg cgcggcgtct ttgtcgcctg gatcggccag gagttgctgc gcctgcgcgc
     1441 cgacctccaa acgcgcaaca acggagggaa cacaattccg taggtgccgg tttaccgcgc
     1501 acaccgccgt tgatgaactt taacgaccaa agccgggaat gtcatgcttc gctgcgctca
     1561 gcatctctcg gcccatcgaa cgagatgctt cgctgcgctc agcatgacaa aacattactg
     1621 atgttggttg tcaaaaatca tcatcggcca cgaggggcgt tagtggcgat aaatcgtagg
     1681 cgaccttttg tacatcgaat ggaaggagtt actgatatgt ctcacctggc tacacctgag
     1741 agcgatgcgc ccttgcaggc cgacgtggtg cttgagggcg gcggcgtgcg cggcattgga
     1801 cacgtgggcg cgctgtgtat cgcggaagag caagggtatc aatgggtcaa tatcgccggg
     1861 acatctgcgg gcgcgttcgt agcagcgatg ctggcggcgg gctatagcgg gcaggaaatg
     1921 tataccatca tgcgcgacga ggtggacttt acgctgttcg cgcgggatag aggcatcgac
     1981 ggactggcgc cggtcgaggc gctgcacctg ctgcgacagg gcggtctgca caacggcagt
     2041 tatatcgaaa gcttcattcg cgggaagata caggcgaagg gcgtcacaac ttttggcgat
     2101 ctcgtgctgc gagaacacga gccgaagcac tcgagcgttc gctatcgcct gacggtgatt
     2161 gcctcggata tcagcagcgg ccagatgctg cgcctgccgc aggatatgag ccagttcggg
     2221 tttgaccccg accgcatcga aatcgcaaaa gcggtgcgca tgagcgccag catcccgttt
     2281 ttcttcatcc ccgtcgcgca aaaagacaac gagggacgaa actgcctgat cgtcgatggt
     2341 ggcctgctga gcaacttccc ggtctacctc ttcgatgtgc agacacagcc gaaacgaccg
     2401 acctttggtc tgcgcctggt cgattcgccg ccaacgccca gctgcccctg gccgttcaac
     2461 ccgaccaaca atatgctgga aatcacccac gcgctgctgc aaaccatgct gaccgcgcac
     2521 gaccggctct acatcgacga ccattcctac gtgcgcacca tcgccatccc ggtcaacggc
     2581 atcagcgaca caaagttcaa cctgagcaag caggaagcca atacgctcta tcagaacggt
     2641 agacacgccg cccaggactt cttctccacc tgggatttcg aggcatacaa ggcagccttt
     2701 cgcggcgacc agccccaaca aagccgccgg gaacgactaa ccgcctccat gaaacaggca
     2761 aagtagcctt atcgtttaat cgccgccagc agttccgaga tcgccatcat ctccatggga
     2821 gtc
//
LOCUS       DASZAK010000083         4056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_605786, whole genome shotgun sequence.
ACCESSION   DASZAK010000083 DASZAK010000000
VERSION     DASZAK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605786"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VGT44_01320"
     CDS             <1..725
                     /locus_tag="VGT44_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579464.1"
                     /translation="TQQRKVRLVRIAISGIDGSGKTTLCGVLTKWLRNLNFPVYLQNL
                     EPRAYQARELTQIIDSDDDRLSSIFFNSAKKLDGDPGFARTRFSKAYVEYIMALEEVK
                     LFERFAVAFDTDSSFVIHDRHVIDRKINALLAGCPADDIKFILGLIRSPDLTFLLDLP
                     VNVSQERLLKTRGHLGADEVPEEQVPLRQAYLETIVDDLSVCLLEASQPANAVFERAK
                     LEILERFSVVGQKARRGSIWTN"
     gene            988..1623
                     /locus_tag="VGT44_01325"
     CDS             988..1623
                     /locus_tag="VGT44_01325"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2579465.1"
                     /translation="MIPIREMSYLWGLVAAETIAMSLPNLTRRIDQEVRSGQLAYLLG
                     RPCSYIFYNFAQYLGERLVRLFLNALVGIVVALIFVGPPHFTWMGVLAWPLVAFLALS
                     IDFVAYFSIGLLAFWTEDTQPFTMILSRMTLVFGGVLAPLDVFPQPLRAIAQVLPFSA
                     ILYGPSRTLVRFEAAQFGWLVFQQLLTLAVGGLILFAVYSISIRRVNINGG"
     gene            1627..2442
                     /locus_tag="VGT44_01330"
     CDS             1627..2442
                     /locus_tag="VGT44_01330"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2579466.1"
                     /translation="MRTVTSLLQAFWRTLRFIGIYMVANAQASMEYRVAFGLQVLTMA
                     ANDSLWLFFWWTFFRQFPLVNGWQSTDIIVIWSISAAGFGVGMSVFGNAPRLAALIMN
                     GGLDAYIGMPRNVLLHVCVSATDPTAWGDVLFGLGAYVLFLHPNVGQVALFLLLCLMV
                     ALLFASFVVILGTLAFFLGNTEGMANQLLGALINFSTYPMNIFSGVVRLLLFTIIPAG
                     FISFIPLQLLRHFAWPLFSGMIGFTALFSCIAAGVFQLGLRRYESGNLMGMQN"
     gene            2536..3597
                     /locus_tag="VGT44_01335"
     CDS             2536..3597
                     /locus_tag="VGT44_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579467.1"
                     /translation="MKKLFWITCLFVFIAPGISMDHQANYAVTSLHVQPMSISSHTSS
                     GLSQRHFFAGFSVNDPATVYSAASVGLGEAQSYADAATVNNALGKAMLSTHMHQIDST
                     LWYYLYQYQCHQNLDSIAANYTDCEKVNPFPTVAALLRGVAAYLQQARNNSLIDAYWV
                     LDDGTLWKPGGAKLLLQQIHRLIQQYSPGRPAICGFGAEFGYRHDDYWMASLAQNFSP
                     QGCDMVGLYIYSAYLNPIRHPSPDQFDWKMTTILPKMFASLRKQGWNQSRQPLIGITQ
                     SFAGMESDRKSYLIVPSVLNMVTQALAFCQDGAIGVSFYAWDIGGEFLNPQTPMNNSQ
                     MAQGVKQSVAACTRYWQTH"
     gene            3681..4022
                     /locus_tag="VGT44_01340"
     CDS             3681..4022
                     /locus_tag="VGT44_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579468.1"
                     /translation="MKGFHKECTWHSDMFWGRDGEIDPDGRILRNEHTLRVILRGLGQ
                     AHRHSSASLEGWRLFLPFVLRTGSNPVGTRDDVRYAHAINAWIKAGRGRELANCHSIE
                     EMYALTRRGRK"
BASE COUNT          862 a         1099 c         1076 g         1019 t
ORIGIN      
        1 tcactcagca aagaaaggtc agactcgtgc gtatagctat aagtggcatt gacggtagtg
       61 gcaagacaac tttatgtggg gtacttacaa aatggttacg caatttgaat ttccctgtat
      121 atctgcaaaa tctcgagccc agggcttatc aagcaagaga attaacacaa attatagata
      181 gtgatgatga tcgcctttca tcgattttct tcaacagtgc taagaaactg gatggcgatc
      241 ctggctttgc tcgcacgagg ttctcgaagg catatgtgga gtatatcatg gcattagagg
      301 aggttaaact ctttgaacgt ttcgcagtag ctttcgatac tgactcttct tttgtcatcc
      361 atgatcgtca tgtaatagat cgcaagatca atgcgcttct agcgggttgc cccgctgacg
      421 acataaaatt cattctgggg ctcattcgat ctcctgattt aacattcttg ttagatttac
      481 cggttaatgt gtctcaggag cgattactca agacgcgagg gcatcttgga gcggatgagg
      541 taccggaaga acaagttcct ttgcggcaag cctatttaga aacaattgtg gatgacctct
      601 ctgtctgtct actcgaagct agccaaccgg caaacgccgt tttcgaacgt gcgaaacttg
      661 agatactgga acgcttctcc gtcgtaggtc aaaaagccag acgaggttca atttggacga
      721 attgaactgt acactagcct cactatctgc aggagtcatc gaacccgctg agccgaaggc
      781 acgagaatta gaaagggatg agcattaata aactgtcgtc caattgatgc gatgaaattt
      841 gctaatctac atcatggcga tctagaccta atatattgat ctttgccaga tggactagcc
      901 tattaagggc tgtcagagag ctttggagtt atgaggtcat gaaaagtcta tccgagttcg
      961 aacggcaact tctcaacgcc ggggtaattg atccccattc gagagatgtc ctacttgtgg
     1021 ggtctcgttg ccgccgagac tatcgccatg tcgctgccca atttgacgcg gcgcatcgat
     1081 caagaggtgc gttcgggaca actggcctac ctgctggggc ggccatgcag ctacatcttc
     1141 tacaacttcg cgcaatacct gggcgagcgg ttggtgcgcc tgtttctcaa cgcgctcgtg
     1201 ggtatcgttg tggcgctcat cttcgtgggt ccgccgcatt tcacctggat gggcgtcctg
     1261 gcgtggcccc tggtcgcgtt cctggcgctg tctatagatt tcgtggccta cttcagtatc
     1321 gggctgctgg ccttctggac ggaggatacg cagccgttca cgatgatcct gagcaggatg
     1381 acgctggtgt ttggcggcgt gctggcgccg ctggatgtct tcccacaacc cctgcgcgcc
     1441 atcgcccagg ttctaccgtt cagcgccatt ctctacggcc cgtctcgcac gctggtgcgt
     1501 ttcgaggcgg cgcaattcgg ctggctcgtc ttccagcagt tgttgacgct ggcggtcggc
     1561 ggcctgatcc tgttcgcggt ttacagcatc tcgatccggc gcgtcaatat caatggaggc
     1621 tagcgaatga gaacggtcac atccctgtta caggcattct ggcgcacgct gcgcttcatc
     1681 ggcatctaca tggtcgccaa cgcgcaggca tccatggagt atcgcgtcgc cttcggtttg
     1741 caagtgctga cgatggccgc caacgatagc ctgtggctct tcttctggtg gacgttcttc
     1801 cgccagttcc cgctcgtaaa cggctggcaa agcaccgata tcatcgtgat ctggtccatc
     1861 tccgcggccg gtttcggcgt gggcatgagc gtcttcggca acgcgcccag gctggctgcc
     1921 ctcatcatga acggcgggct ggatgcctac atcggtatgc cgcgtaatgt gctgctgcac
     1981 gtctgcgtct ccgctaccga cccgacggcc tggggcgatg tcctcttcgg gctgggcgcg
     2041 tatgtcctgt tcctgcatcc caacgtggga caggttgccc tgttcctgct gctctgcttg
     2101 atggtcgcgc tgctcttcgc ctcgttcgtg gtgatcctcg gcacgctggc ctttttcctg
     2161 ggcaacaccg agggcatggc gaatcagctg ctgggcgcgc tgatcaactt cagcacctat
     2221 cccatgaaca ttttcagcgg cgtcgtcagg ctgctgctct tcactatcat accggctggc
     2281 ttcatctcgt tcatcccgtt gcaactgctc aggcacttcg cctggcctct gttctctggc
     2341 atgatcgggt ttaccgccct gttctcctgc atcgccgccg gggtattcca gcttggcctg
     2401 cgccgctacg aaagcggcaa tctgatgggg atgcagaact agcgccagtt cgcaaacgct
     2461 ctataaatct ctctcacctt ccgatatata tatatcaaga agcttgaata tatccgtaag
     2521 gagaagagac cacccttgaa aaaattgttc tggatcacct gcctcttcgt atttattgcg
     2581 cctggtatta gcatggatca ccaggcgaac tatgccgtga catcccttca tgttcaaccc
     2641 atgtcaatat cttctcacac ctcgtctggc ctttcccaac gtcacttctt cgcagggttt
     2701 agtgtcaacg atccggcaac agtctattcc gccgccagcg tgggcttggg cgaagcgcag
     2761 tcgtatgccg atgccgcgac tgtcaataat gctctgggca aggctatgct gtcaacgcat
     2821 atgcaccaga tcgactctac gctctggtat tatctctacc agtaccagtg ccatcagaac
     2881 ctggattcca ttgccgcaaa ctacacggat tgcgaaaaag tgaatccttt tccaacggtg
     2941 gctgctttat tgcgtggggt cgctgcctat ctacagcagg cgagaaataa tagcctgatc
     3001 gatgcctact gggtgcttga cgatggaacg ctgtggaagc ccggcggcgc gaagctgctc
     3061 ctgcaacaaa ttcaccgcct gattcaacaa tattcccctg gaaggccggc catttgtggc
     3121 tttggcgctg aatttggcta ccggcacgat gattactgga tggcttcact cgcgcaaaac
     3181 ttttcgcccc agggctgtga tatggttggt ctgtacatct actctgccta cctgaatccc
     3241 atcaggcatc cctcgcctga ccagtttgat tggaaaatga ccaccatttt gccgaagatg
     3301 tttgccagtc tgcggaaaca gggctggaat caaagtaggc agccccttat cggcatcacc
     3361 cagtcctttg cgggtatgga gagcgaccgg aagagctacc tgatcgttcc cagcgtgctg
     3421 aatatggtga cgcaggccct cgccttctgt caagacggag ccatcggcgt atccttttat
     3481 gcctgggata taggaggcga attcctcaat ccccaaacac cgatgaacaa cagtcagatg
     3541 gcgcagggtg tgaaacaatc ggtagccgcc tgtacgagat attggcaaac gcattagaac
     3601 cgagggcatc gagctcagag tggaggactt gcgtgtcttt gcatcaggtg gttccgttgg
     3661 agtaaactgg agcggaagca atgaaaggat ttcacaagga atgtacctgg cacagtgata
     3721 tgttctgggg cagggatgga gaaattgacc cggacgggcg gatactccgt aatgaacaca
     3781 ccttgcgcgt catactgcgt ggcctcggcc aggcgcatcg ccacagctcc gcctcccttg
     3841 aaggctggcg actctttctc cctttcgtct tacgcactgg ctcaaatccc gtaggtacga
     3901 gggatgatgt gcgctacgct cacgccatca atgcctggat caaagccggg cgcgggcgcg
     3961 aactggccaa ctgtcacagc attgaggaga tgtacgctct tacgcgccgt ggcagaaaat
     4021 aacccctgtt caaattaaag ttcgttcaga catttg
//
LOCUS       DASZAK010000084         4842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_499038, whole genome shotgun sequence.
ACCESSION   DASZAK010000084 DASZAK010000000
VERSION     DASZAK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499038"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VGT44_01345"
     CDS             complement(<1..896)
                     /locus_tag="VGT44_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV2579469.1"
                     /translation="MIEKDQPQVLDEADPSLLVRPEVRPGAFPGNERIRFVYPRQPTL
                     RRVRRGVLEATAAAQAPHGVWGRIKRVVIGTPIPTSLAVHERLTKFKALAVLSSDVIS
                     SVAYATEAILATLIAAGSGTLGLTLPIAIAIVCLLGIVALSYRQTIPAYPNGGGSYIV
                     AKDNLGTVPGLVAAASLMIDYVLTVSVSVAAGVQALATLFPALSSTFTIVSIDVALVL
                     LIMMVNLRGVRESGSIFAIPTYIFIASALLLIVVGFFKSFVFQHNPLIGSFAYAQTTE
                     PLSIFLILKSFAAGCSALTGTEA"
     gene            complement(889..1506)
                     /locus_tag="VGT44_01350"
     CDS             complement(889..1506)
                     /locus_tag="VGT44_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579470.1"
                     /translation="MTETIRLLLPFSHGVEIDTIEAAVLLAASHHATLVPLSLIRSPQ
                     TRGKGARLEHIQQSKDFLEAVQQKALRHGVPFERFEVFTSDALQSISVLVHQLMCDGV
                     VIALRGRNGSLLHVEMIEQLKAMKPCPLYLLYLPSRETSWVSRLRERFTRWRPGQRQR
                     AVRRVQLQPEIEEMGDQQVGLTQHTASPELAGEHKRVLGGNRYDD"
     gene            complement(1656..2528)
                     /locus_tag="VGT44_01355"
     CDS             complement(1656..2528)
                     /locus_tag="VGT44_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HEV2579471.1"
                     /translation="MRTTTRLRELLAAPDLLVAPGAYDALSARLIAQAGFPAVYMTGF
                     GTSASVLGQPDVGLLTMSEMLSRAAALASVVGDIPLIADADTGYGNPINVRRTIREYE
                     RAGVAGLHIEDQVWPKKCGHMEGKQVIPMDEMVQKVRAAADARQDPDFVIIARTDANA
                     VTGFEDALRRGQAYREAGADVVFIEAPRSIEELRAIGQAFRDVPLLFNWADSGKTPPL
                     PLNEIQALGFKLVIFPVSLLFAATHAMLAMLELLKTGATPTAFSEHMVTFSQFTTQIG
                     LPDIQALERRYGVQ"
     gene            complement(2698..3789)
                     /locus_tag="VGT44_01360"
     CDS             complement(2698..3789)
                     /locus_tag="VGT44_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV2579472.1"
                     /translation="MSEARRKAIVIHSPYSGRSARLEDALAHLREAGIEVVEQMAVSE
                     LDGLPPQGSYWQKSGINLAIAAGGDGLIGGVITHIAPSGLPLGIMPLGTANDIARSLN
                     IPQDIDLAAQIIRAGRVQEVDVGVAKPSEQAPHLTGKGALAPLLAHISTRKHGYFAHA
                     LTIGLNVQFARIATNIATRQRYGRLTYPIAALETLKYRDALDVSLRFEGLAFPTDHDA
                     KQAKEMEELQSISYQALQITVINAPIFGGQWKLSVPNASIHDRLLDIVVIEEIEFSRL
                     NTALTHFLYSLRQDHPSNNEPPPLAPHPAELSAIPGIHHFQARGVLISTTADPRDVTL
                     DGEVRGQTPIYVHLADERLRVVVPQDGDV"
     gene            complement(3816..4775)
                     /locus_tag="VGT44_01365"
     CDS             complement(3816..4775)
                     /locus_tag="VGT44_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2579473.1"
                     /translation="MPPLLSLPAVDGVEITTIMDNSLDVLMASTPVAHRYPLPQNLFE
                     RDQLRAEHGASFLLTVINQGRRETILFDTGVSPDGALHNIDVLGVDLSAIQAIVLSHG
                     HTDHTQGIYGFLDRLGRRHMPILLHPDAFLKRHIVVGEGKLVDLPPPSLHDLSREGIE
                     LLVERGPSLLVNGTVLVTGQIERTTDFEQGMKNQQAEIDGVWQPDPWVYDDQAIVVHV
                     RDKGLVVLTGCGHAGVINILRQARAQTGVEQIYAVLGGLHLTGALFEPIIPQTIAELQ
                     RIAPSVVVPAHCTGWKATHGIARAMPEAFTPNSVGTTFALMAG"
BASE COUNT          981 a         1420 c         1484 g          957 t
ORIGIN      
        1 gcctcggtgc ctgtgagcgc cgagcagccc gcggcaaagg atttcaggat caggaagatc
       61 gagagtggtt cggtggtctg cgcataggca aaggaaccga tgagggggtt gtgctggaag
      121 acaaaggact tgaagaagcc caccacgatg agcaggaggg cgctggcaat gaaaatgtag
      181 gtgggaatgg caaagatcga gcctgactcg cgcacaccgc gcaggtttac catcatgatc
      241 agcaatacca gcgccacatc gatgctcaca atggtgaatg tagaagataa ggccgggaag
      301 agcgtggcga gcgcctgcac accggcggcc acgctgaccg agacggtgag gacataatcg
      361 atcatgagcg aggcggcggc caccagtcct ggcacggttc ccaggttgtc tttcgccacg
      421 atgtaggacc cgccaccatt ggggtaggcc gggatggttt ggcggtagga aagcgccaca
      481 atgcccagca ggcacacgat ggcgatcgcg ataggtaagg tcaaccccag ggtccccgaa
      541 ccggcggcga tgagggtcgc cagaatggcc tcggtcgcat aggcgaccga cgagatcacg
      601 tccgacgaga ggacggccag ggccttgaat ttggtcagcc gttcgtggac cgccagcgag
      661 gtggggatgg gagtgccgat caccacgcgc ttgatgcggc cccacacgcc gtgcggagcc
      721 tgggctgcgg cggtcgcttc gaggacgccc cggcggaccc gccgcaaggt gggctggcgc
      781 ggatacacaa agcggatgcg ctcgttgcca gggaacgccc ccggacgcac ctcagggcga
      841 acgagcaggc tcggatcggc ttcatcaagc acctgtggct ggtctttttc aatcatcgta
      901 tctattccct cccaatacac gtttgtgctc gcctgccagc tcaggggaag cggtgtgctg
      961 agtcaaccct acttgctggt ctcccatttc ttcgatctct ggttgcagtt ggacgcgccg
     1021 gactgcgcgt tgccgctgtc cgggccgcca gcgggtgaaa cgctcgcgca gccgcgacac
     1081 ccaggacgtt tcccttgaag gaaggtacag gagatagagc gggcaaggtt tcatcgcctt
     1141 gagctgctcg atcatctcaa catgcagtaa actcccgttc cgaccacgga gcgcgatgac
     1201 tactccatca cacatgagct gatgcaccag gaccgaaata ctctggaggg catcactggt
     1261 aaacacctcg aagcgctcaa aagggactcc atgccgtaga gctttttgct gcacggcctc
     1321 aagaaaatct ttggactgct ggatgtgttc caggcgggcc ccttttcctc tcgtctgagg
     1381 cgaacgtatc agggagaggg gaacgagcgt ggcatgatga cttgcagcca ggagtacggc
     1441 agcttcgata gtatcaatct ctaccccgtg cgagaacgga agcagcaatc ttattgtttc
     1501 ggtcatctct tcaggcccaa ctagctagta cacaacatca agttgtcttc atttgtactt
     1561 aaccatagcc cataggagat caacacacgc tcaagaaagc tgaggggtat ctcaagattt
     1621 gctcaaaatc tggggggaag aggatatccg tgtttttact gaacaccgta gcggcgttcc
     1681 aatgcctgga tatcgggcag gccaatttga gtcgtgaatt gcgagaacgt caccatatgt
     1741 tcgctgaatg ccgtgggcgt cgcgcctgtt ttgagcagct ccagcattgc tagcatggca
     1801 tgtgtggcgg caaaaagcaa gctgacagga aaaataacca gtttgaagcc gagcgcctgg
     1861 atctcattga gcggcagcgg aggcgttttg ccgctatcgg cccagttgaa gagcagcggc
     1921 acgtcccgga aggcctgccc gatggcgcgc agttcctcta tcgaacgcgg tgcctcgatg
     1981 aagaccacgt ccgcgccggc ctcgcggtac gcctggccgc gccgcagggc atcctcgaag
     2041 ccggtgacgg cgttggcatc tgtgcgcgcg atgatgacga agtcggggtc ctggcgcgca
     2101 tcggcggcgg cgcgcacctt ctgtaccatc tcatccatcg ggatgacctg cttgccctcc
     2161 atgtggccgc acttcttcgg ccacacctgg tcttcgatat gcaggcccgc gacccccgcg
     2221 cgttcgtatt cgcggatggt gcggcgcacg ttgatggggt tgccatagcc tgtgtcagcg
     2281 tccgcgatga gcggaatatc gcccaccact gacgccaggg cagccgcgcg gctcagcatc
     2341 tcgctcatcg tcagcagtcc aacatccggc tgtccaagca cgctggctga tgtgccaaac
     2401 cccgtcatat acacggcagg aaagcccgcc tgtgcgatca aacgcgcgct cagcgcatca
     2461 tacgctccag gggcgaccag caggtcggga gccgccagca gttcgcgcaa acgagtagtg
     2521 gtacgcattg ttgagagagt cctttccgcg aaaacgtgtt gagaatgcct gtgtgctgat
     2581 agtgtatgat acaaggagga cgagggcgac cgcaaggggt ggagaccatt cggcgactca
     2641 tccgacaacg agcagggcga ccgccagggt ctccacccct tgcggtcgcc ctgggaatta
     2701 cacatcgccg tcttgcggta cgaccactcg cagccgttcg tcggccaggt ggacgtatat
     2761 gggtgtctgg ccgcgcacct cgccgtccag cgtcacatcg cgtgggtcgg cggtggttga
     2821 gatgagaacg cccctggcct ggaaatggtg gatgcccggt atggcagata gttcggctgg
     2881 gtgaggagcg agcggcggtg gctcgttatt ggatgggtga tcttgccgga gcgagtagag
     2941 gaaatgggtc agggctgtgt tgagcctgct gaactcgatt tcttcgataa ccacgatatc
     3001 cagcaagcga tcgtggatgc tggcgtttgg tacgctcaac ttccattgcc cgccgaatat
     3061 cggcgcattg ataaccgtga tttgtagcgc ctggtaggag atggattgca gttcctccat
     3121 ctcttttgcc tgctttgcat catgatcggt cgggaaagcc agtccttcga aacgcagcga
     3181 tacatcgagc gcatcgcggt atttcagcgt ctctaaagca gcgatggggt aggtcaaccg
     3241 tccgtagcgc tggcgcgtgg cgatgttggt ggcgatacgc gcaaattgca cgtttagccc
     3301 gattgtaagg gcatgggcaa agtagccatg tttgcgggtg gaaatatgcg ccagcagtgg
     3361 ggcgagcgct cccttgccgg tgagatgcgg cgcctgctcg gacggtttcg ccacgccgac
     3421 atcgacctcc tgtacccgac cggcacgaat gatttgggct gccagatcga tatcctgggg
     3481 gatgttgagg gagcgcgcga tgtcgttggc ggttccgagt ggcatgatgc ccagcggcag
     3541 cccgcttggg gcgatgtgcg tgatgacccc gccgatcagg ccgtcgccgc cagccgcgat
     3601 tgccaggttg atgccgcttt tttgccagta actgccctgc ggaggcaagc catccagctc
     3661 gctgaccgcc atctgctcta ctacctcgat cccggcctcc cgcaaatgcg ccagggcgtc
     3721 ctccaggcgc gctgatcttc ctgaatatgg gctatggatc acgattgcct tgcgtcgtgc
     3781 ctcactcata catatgtcca tccttgtgtg tcatcttatc cggccatcaa cgcgaaggtc
     3841 gtcccaacgc tgttcggcgt gaacgcctcc ggcatggcgc gcgcgatgcc atgcgtcgcc
     3901 ttccaaccgg tgcagtgcgc cggcacgacg accgaagggg cgatgcgctg caattcggcg
     3961 atggtctgcg ggatgatcgg ctcgaacagc gcgccggtga ggtgcaagcc gcccagcacg
     4021 gcgtaaatct gctccacgcc ggtctgcgcc cgcgcctgtc gcaggatgtt gatgactccc
     4081 gcgtggccgc agccggtcag cactaccagt cccttatcgc gcacgtgaac cacaatggcc
     4141 tggtcatcat acacccaggg gtcgggctgc cagacaccgt cgatctccgc ctgctggttc
     4201 ttcataccct gctcaaagtc ggtcgtgcgc tcgatctgcc ccgtcaccag caccgtccca
     4261 ttcaccagta acgatggccc gcgctccacc agcagctcga taccttcgcg cgacaggtcg
     4321 tgcagcgatg gcgggggcaa atcgaccagc ttgccttctc caacaacaat gtggcgcttg
     4381 agaaacgcat caggatgcag cagaatcggc atatgtcgcc tgcccagccg atcaagaaaa
     4441 ccgtagatgc cctgtgtatg gtcagtatga ccatgactca gcacgatggc ctggatcgcg
     4501 ctgagatcga cgcccagcac atcaatgtta tgcagcgctc catccggcga gacgcccgta
     4561 tcgaacagaa tcgtctcacg cctgccctgg ttgatgaccg tcagcaaaaa cgacgcccca
     4621 tgttcggcgc gcaactggtc gcgctcaaat aaattctgcg gcagcggata acgatgcgcc
     4681 accggcgtcg aggccatcaa cacatccagg ctgttgtcca taatcgtcgt aatctcgacg
     4741 ccatccacgg cgggcaagct cagtagaggt ggcatatacg ctcctttcgc aatgttgctg
     4801 ttcatgaaga gcatgtttgc attgtatcac gtaccggcgt ag
//
LOCUS       DASZAK010000085         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_108842, whole genome shotgun sequence.
ACCESSION   DASZAK010000085 DASZAK010000000
VERSION     DASZAK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108842"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VGT44_01370"
     CDS             <1..1000
                     /locus_tag="VGT44_01370"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2579474.1"
                     /translation="TLNAQAVQQIVHAFLANVETTRTLRREYPEMRMKYPWRTKRFYP
                     VHWPAQAVSVERGRVVLPMGRGRASLVFHLDLPANIGACTLVWNRGFELHVCIEVPRA
                     EQAPGKVHATVDLGEIHLAAVTTNTGAAIVVSGRGIRSLKRQRNMRHRQLTKKQARCQ
                     KYSRRWRKLQRAKNRMAGRIERRVRDLRHKATRQVIEFCREQQVGTLFIGNPHGVRQE
                     NGGRHHNQRMAQWEYGKDIDYLTHKSKHARIESFTGSERGTSSQCPVCRHRHKPRGRV
                     WKCKKCGFKGHRDVVGSVNMHRLAYDEQVTFPRSVTYLRPGPSRRSSRAGTPRASS"
     gene            complement(1121..>2495)
                     /locus_tag="VGT44_01375"
     CDS             complement(1121..>2495)
                     /locus_tag="VGT44_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV2579475.1"
                     /translation="EPIPSPRALCPDMPETVEMVVRVALAKTPQARFPDAESLSRALL
                     AALVKDEPPPVVAATHTITPRRRVQGRRGYAGQPPSVWSRVVTLTSVFVVLAGLIGVM
                     LFFAAVPFRLQDLRFLPFHFPPAGPGGLSLSPVTTSTPVVPPLLPGQVGPSSGGTTSN
                     FPGTATPEPGVSPTMGATAMPTAIPTLTATSTPIPLVCANGTLSIDGSPYLAPILALI
                     NQDYASGCSGMTLTMRNDGIRALNFVQNGRVDVADTDVTANASRNLVDHPIAALLYSL
                     IVSPDVALSGVNNLSLADIQDIYAGQITNWSQLGGPDERIIVVYPPPNASINAIFRAF
                     VLGGVPPQAKGYTLKRDNPNVTAQLVAQTPGAISYVPLETLSTAHAQPLSINGFSASS
                     QTLLSGDYPFWSVEHVYTLGFGTTQAQDWLQFLPQESNTMLVSGAVPVSMLSPGILLS
                     HLPGPQF"
BASE COUNT          524 a          720 c          842 g          409 t
ORIGIN      
        1 cacactcaat gcccaggccg tgcagcagat tgtccacgcc ttcctggcca acgtggagac
       61 gacgcgcacc ttgcgccggg agtaccccga gatgcgcatg aagtacccct ggaggacgaa
      121 gcgcttctat ccggtccact ggcccgcaca ggcggtcagc gttgagcggg ggcgcgtggt
      181 gctgccgatg ggaagaggac gcgcctccct ggtctttcac ctggacctgc cagcgaacat
      241 cggggcctgt acgctcgtct ggaatcgtgg ctttgaactg cacgtctgca tcgaggtgcc
      301 acgagccgag caagcccctg gcaaagttca tgcaaccgtt gacctcggag aaatccatct
      361 cgccgccgtg accacgaata cgggggcggc catcgtcgtc tcggggcggg gcatccgcag
      421 cctcaagcgt caacgcaaca tgcgccaccg gcaactcacg aagaagcagg cccgctgtca
      481 gaagtactcc cgccgctgga gaaagctgca acgtgctaag aaccgcatgg cgggccgcat
      541 cgagcggcgg gtgcgtgacc tgcgtcacaa ggcgacccgg caggtgatcg agttttgcag
      601 agagcaacag gtcgggaccc tcttcatcgg caacccacac ggcgtccgcc aggagaacgg
      661 agggcggcat cacaaccagc gcatggcgca gtgggagtac ggcaaagata tcgactacct
      721 cacccacaag agcaaacacg cccgcatcga gagcttcact ggttcagaac gagggacgag
      781 cagccagtgt ccggtatgca ggcatcggca caagccgaga ggcagagtgt ggaagtgtaa
      841 aaagtgtggg ttcaagggac accgtgatgt cgtcggcagc gtcaatatgc accggctggc
      901 ttatgatgag caggtgacct ttcctcgttc ggtcacgtat ctacgccctg gcccgtccag
      961 gcgtagtagt agggcaggca cgccccgtgc atcctcgtaa tgcatgttgt cttggtgagt
     1021 caatgggtca accacctctt cagcagagag ggtctcgcga gggacaggcc acaccggcga
     1081 agtcactaag aaacttatct cccctgagga gataagggtg tcagaattgg ggtccgggca
     1141 ggtgggacag tagtatccca gggctgagca tggaaacggg gaccgcgccg gagacgagca
     1201 tcgtattcga ctcctgcggg agaaactgta gccagtcctg tgcctgcgta gtaccaaatc
     1261 ccagcgtata gacatgctcg acgctccaga agggatagtc accggaaagc agcgtctgcg
     1321 agctggccga gaatccattg atggacaacg gctgcgcgtg cgccgtggag agcgtttcga
     1381 gcgggacgta actaatcgcg ccgggcgtct gtgcgaccag ttgtgcagtt acgttggggt
     1441 tatctcgctt gagggtatac cctttagcct ggggcggcac gccaccaagg acgaatgcgc
     1501 gaaaaatggc attgatcgag gcgttcggcg gcgggtacac cacaatgatg cgctcatcgg
     1561 ggccacctaa ctgcgaccag ttggtgatct ggccggcata aatgtcctgg atatccgcga
     1621 ggctcaagtt gttcaccccg ctcagggcca catcggggct gacaatcaac gagtagagca
     1681 gcgcggcaat ggggtggtcg acgaggttac gcgacgcatt ggcggtcacg tccgtatccg
     1741 cgacatcgac gcggccattt tgcacgaagt tgagggcgcg gatgccgtca ttgcgcatgg
     1801 tcaaggtcat gcctgagcag ccgctggcgt aatcctgatt gatgagggcc aggattggcg
     1861 cgagataggg cgatccatcg atggacagcg tgccattcgc gcacaccagc ggaatgggcg
     1921 tactcgtggc agtcagcgtc ggtatggcgg tcggcatcgc cgtcgcgccc atggtggggg
     1981 agacaccggg ttcgggcgtc gcggttcctg ggaagttgga ggtagtgccg ccgctcgacg
     2041 ggccgacctg accgggaagc aggggcggca ctaccggcgt agaagtggtc acgggactca
     2101 ggctgaggcc gccgggaccg gcggggggaa agtgaaacgg caaaaaccgc aggtcttgca
     2161 ggcgaaatgg cacagcggcg aaaaagagca tgacgccgat caagcctgcc agcacgacaa
     2221 acaccgaggt caacgtcacg acgcgcgacc agaccgaggg cggttgcccg gcatagcccc
     2281 tgcgcccctg cacacggcgg cgcggcgtga tggtgtgcgt ggctgccacg acgggcggcg
     2341 gctcgtcctt caccagggcg gccagtaacg cgcgggagag tgattcggca tcagggaaac
     2401 gtgcctgggg cgttttggcc agcgcgacac gcacgaccat ctcgaccgtt tcgggcatgt
     2461 cgggacacag cgcgcgcggc gaggggatcg gctca
//
LOCUS       DASZAK010000086         3486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_534720, whole genome shotgun sequence.
ACCESSION   DASZAK010000086 DASZAK010000000
VERSION     DASZAK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534720"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..494
                     /locus_tag="VGT44_01380"
     CDS             144..494
                     /locus_tag="VGT44_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEV2579476.1"
                     /translation="MRYLLMIYQDEAEHAKWSQEQLAAEYQAFNVFTEQLSKSGAMVG
                     GEALMPTNTATTVRVRNGKTLTTDGPFAETKEQINGYYLVNCKDLDQAIAAAAMIPTA
                     TSGSIEIRPVMEFE"
     gene            523..1785
                     /locus_tag="VGT44_01385"
     CDS             523..1785
                     /locus_tag="VGT44_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2579477.1"
                     /translation="MNAAQQAVSDTFAREADRILAALIGTVRDFELAEDALQDALLVA
                     LERWPVDGIPRNPSAWITTTARHRAIDRLRRANVQARKQVVLQALLEEEEQRGEEEMD
                     DTSIPDERLKLMFTCCHPALAQEAQIALTLHTLGGLQTPEIASAFLVPLPTMAQRLVR
                     AKRKIRDAGIPYRVPEAALLGERVDAVLYVLYLIFNEGYMATSGDQLIRRELCEEATR
                     LARVLTQLLQQESLTSEVPEAMGLLALLLLHDSRREARVGPTGELVLLEDQDRSRWDR
                     AKITEGIAILERALRMRQPGPYQLQAAISALHAQAARAEDTDWPQIAALYTELMKMMP
                     SPVIELNRAVAIAMADGALRGLKLLDQPLLAKELSDYYLFHATRADLLRRLGRMSDAR
                     DAYMVALGLCQNERERAFFKRRLVEVSG"
     gene            complement(1959..>3486)
                     /locus_tag="VGT44_01390"
     CDS             complement(1959..>3486)
                     /locus_tag="VGT44_01390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579478.1"
                     /translation="GTGTFEGVRPEDMAQRQINNAIPPLSRTRSDLPPSMDNVLAGAL
                     AKEPAQRYGQPGAFANAYHLSGVPTNRMRVPFVVAETPAARIHQTPYSNPSRMDTPFY
                     ERGWNGNSSNGISGEDHTVVQTRSPSQSPGSHSLHGFPEDEPLSLLDAPRPAMMRRFG
                     SRQRQRTLIIASLVALLVIGSVITGFALLAQKGVATAHTTGVVTFFASSPEAQTNELR
                     LSIGNLPTPPSGSVFDAWIIDDHTEQVLGLGTLANNGQSWTLTFNAENTDLLAVGDKI
                     EVTQEQGKVTVPTGQIMLVGTFPANSYAHVLHLLVAFPGTPGQVGFLIGLLAQSHLLD
                     NQAAVLQSVFASKNTNAIECVTQSMLDIIGGSHDPHYKPLTNGCAQQNVTAAGDGFGM
                     LGKAGYVAGVEEHASLALSQSDATVAMHQHATLMNVALTNISGWVTTVQQDLLQLQAH
                     PTDQSSMQQIVMMADNAYHGVDINGDGQIDPVPGEAGAITAYQQGQLMATVTLSPPSA
                     "
BASE COUNT          732 a          955 c         1053 g          746 t
ORIGIN      
        1 gaggaggtgt atgatcggta tattcgggcg caggtgcagg atcggtggta gcagtgtcga
       61 ttcggcttta tccaaatcgt cgttataatg aagggacaaa ctgatagccc tttgatatac
      121 ttgtttgaag aaagggaaac aacgtgcgtt acctactgat gatttaccag gatgaggccg
      181 aacacgcgaa atggagccag gagcaactgg ctgccgagta ccaggcgttc aatgtgttta
      241 ccgagcaact gtcgaaaagc ggcgcgatgg tgggcggaga agcgctcatg ccgactaaca
      301 cggccacgac cgtgcgggtg cgcaacggca agacgctgac aaccgatggc ccctttgcgg
      361 agacgaagga gcaaatcaac ggctactacc tggtgaattg caaagacctc gaccaggcaa
      421 ttgccgccgc cgcaatgatt ccgaccgcca cgagtgggtc aatcgagatt cgccctgtca
      481 tggaattcga gtagcggacg gcaatcagga aatgagcagg ccatgaacgc ggctcaacag
      541 gcggtgtctg acacctttgc gcgcgaagcg gaccgcatcc tggcggcatt gatcggcaca
      601 gtgagagatt tcgagctggc cgaagatgcg ctccaggatg cgctgcttgt agcgctggaa
      661 cgctggccgg tcgatggcat tccacgcaat ccgagcgcct ggatcaccac gacggccagg
      721 catcgggcca ttgatcgctt gcgcagggcg aatgtacagg cacgcaaaca ggttgtgctt
      781 caggcattgc ttgaagaaga ggagcaaaga ggagaggaag agatggacga tacctcaatt
      841 cccgatgagc gcctcaagtt gatgttcacc tgctgtcacc ccgcgctggc gcaggaggcg
      901 cagattgcct tgacgctgca taccctgggc ggtttgcaga ctcctgaaat tgccagcgcc
      961 ttcctggttc ccctgcctac aatggcgcag cgcctggtac gagccaagcg caagattaga
     1021 gatgccggga taccgtatcg cgtgccggaa gcagcgctgc tgggagaacg agttgacgcc
     1081 gtcctctatg tactctacct gatcttcaac gagggctata tggcgacatc cggcgaccag
     1141 cttatccggc gcgaactgtg cgaggaggcg acgcgactgg cgcgcgtttt gacgcagctc
     1201 ctgcaacagg aatctttgac gagtgaagtt cctgaagcta tgggactgct cgcattgctg
     1261 ctgctgcatg attcgcgtcg cgaggcgcgg gtcgggccga cgggcgagtt ggtattgctg
     1321 gaagaccagg accgctcgcg ctgggaccga gccaaaatta cggagggcat cgccatcctg
     1381 gaacgcgcgc tacgtatgag gcaacccggt ccctaccagc tccaggctgc catcagcgcg
     1441 ctccatgccc aggcagcgcg cgcggaggat accgattggc cgcagatagc agcactgtat
     1501 accgagctga tgaaaatgat gccttcgcct gtgattgaac tgaaccgtgc agttgcaatc
     1561 gcgatggccg acggcgcgct gcgcggcctc aaactgctgg atcaaccgct tttggcgaaa
     1621 gaactgtccg attattacct cttccatgcc actcgcgccg atttgctgcg gcggctcggt
     1681 cgcatgagcg atgcgcgcga cgcctatatg gtggcgctgg gcctctgcca gaatgaacgg
     1741 gagcgcgctt tcttcaaacg acgcctggta gaggtgtccg gctaatgcca ggtcagaatg
     1801 ttctttttac atagcaacaa gcagaccacc aacgattatc acacttgctc caacgctcgc
     1861 cgcaactcat cgccttccta cgctggctgt tcaggcagat attgtgctgc cagcagtaag
     1921 tcgtttatgg tcgcccctac gggtctgttg aagtcgggtt aggcgctggg aggcgatagg
     1981 gtgacggtgg ccatcaattg tccctgttga taggctgtga tggcgcccgc ttctcctgga
     2041 accggatcga tttgcccgtc gccgttgata tccacgccgt gataagcgtt atcggccatc
     2101 atgacaattt gctgcatcga tgattgatcg gtcgggtgcg cctgcagttg tagcaggtcc
     2161 tgttggaccg tcgtcaccca cccggagatg ttcgtcagag cgacattcat caaggtggcg
     2221 tgctggtgca tggctactgt ggcgtcagac tggctgagcg ccagtgatgc gtgttcttct
     2281 acaccggcaa catagccagc tttccccagc ataccaaaac catcgcctgc tgcggtcacg
     2341 ttttgctggg cacacccatt agtaagtggt ttgtagtgcg gatcgtgcga tcctccaatg
     2401 atgtcgagca tactttgtgt gacgcattca atcgcgttcg tgtttttgct ggcaaacaca
     2461 ctttgtaaga cggcggcctg gttatcaagc aggtgtgact gcgccaggag gccaatcaag
     2521 aaaccaacct ggccaggggt cccgggaaag gcgacgagca aatgcaggac gtgcgcatag
     2581 gaattagccg ggaaggttcc gaccagcata atctgcccgg ttgggactgt taccttgccc
     2641 tgttcctgcg tgacctcgat tttgtcgccg acggcaagca agtcagtatt ctcggcgtta
     2701 aaggttaacg tccaggattg accattattc gctagcgttc ctaaccccag cacttgttcg
     2761 gtgtgatcgt cgatgatcca ggcatcaaac acgctgccgg agggaggcgt tggcaggttc
     2821 ccgatgctga gacgaagctc attcgtctgc gcttcaggac tgctggcgaa gaaggtcact
     2881 accccggtcg tgtgagctgt tgccacgccc ttttgcgcga gcagcgcgaa gccagtgatg
     2941 acgctgccga tcaccagcag cgcgaccagc gatgcgatga taagagtgcg ctgtctttgc
     3001 ctgctgccaa agcggcgcat catggccggt cgtggggcat ccaacaggct caaaggttca
     3061 tcttcaggga agccgtgcag tgagtgcgag ccgggcgatt ggctcggcga acgcgtctgt
     3121 acaactgtat gatcctctcc gcttattccg tttgatgagt taccgttcca gccacgctcg
     3181 taaaaaggcg tatccatcct ggatgggttg gaatatggcg tctggtgaat gcgcgcggct
     3241 ggtgtctcgg ccacgacaaa cggtacgcgc atcctgtttg tcggcactcc gctgagatga
     3301 taggcattgg caaatgcccc tggctgacca tatcgctgag ccggttcttt cgccagcgcg
     3361 cctgccaata cgttgtccat gctcggagga agatcactgc gtgtcctgct cagcggtgga
     3421 atggcgttgt tgatctgacg ctgagccatg tcttcaggtc gaacaccctc gaaagtaccc
     3481 gtcccg
//
LOCUS       DASZAK010000087         3441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_499099, whole genome shotgun sequence.
ACCESSION   DASZAK010000087 DASZAK010000000
VERSION     DASZAK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499099"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..800)
                     /gene="lexA"
                     /locus_tag="VGT44_01395"
     CDS             complement(171..800)
                     /gene="lexA"
                     /locus_tag="VGT44_01395"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEV2579479.1"
                     /translation="MGTDHTSDIQKRIFDFIVEYIREEGMPPTNREIGSAMKIASTGH
                     VDYHLSMLEKKGLIDRESKKSRGIKLRTNPFGIHVMGSIAAGEPIEVFSDPVEPLEVG
                     REFEQPGAYALVVKGQSMIEDHICDKDYVVIKPQSTCENGDIVVAVHMQGNGRATLKR
                     FYQETDHNRVRLQPANSEMGPIYIPRKEWDSEWQVQGKVIAILRKCRAA"
     gene            1179..1706
                     /locus_tag="VGT44_01400"
     CDS             1179..1706
                     /locus_tag="VGT44_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEV2579480.1"
                     /translation="MIEMTVESVRINLQNSQRVVILKAEQHERYLFIWIAPAEAYAIA
                     IELQGTSSPRPLTHDLMKNIINDLGATITSVVISDLINDIYYARIVLDVAGRHVEVDS
                     RPSDAIALAVRAHTPIYVEESVLERAGVALETNGDTVTPKIDQHKDREIEYDNLDAYR
                     DFINSLDALDEFGKD"
     gene            1713..2096
                     /locus_tag="VGT44_01405"
     CDS             1713..2096
                     /locus_tag="VGT44_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579481.1"
                     /translation="MEARERLIRQLLLKQRCQLCHSVYTTEGNVILARRRDVWLVMAS
                     CAFCQQKDTFVIQFSAPIRGRRRITSYRLSNPATSNTPSGAPRTQSHPSRQAKRPISS
                     NDVQEMREFLTRFDGDFQRLFAEVE"
     gene            complement(2111..2677)
                     /locus_tag="VGT44_01410"
     CDS             complement(2111..2677)
                     /locus_tag="VGT44_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579482.1"
                     /translation="MLRTISEQQNAMYSAFDDQSRNENDFDSPTDPMIPVILAPHSPD
                     PTDAAHAAEPTAALSPVFHPFQSAPETPLDNPVLPSLPGVPAQPRRSPAPTVVGVCFV
                     AVQIFLLVRVILLLFNVSSSLLWAKVLYAAGGVFALPFRLLLDQIQLLSQLGPGVVNY
                     LAPLAAILVYGLISRILVRFLKALLNSR"
     gene            complement(2844..>3441)
                     /locus_tag="VGT44_01415"
     CDS             complement(2844..>3441)
                     /locus_tag="VGT44_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2579483.1"
                     /translation="FCAGADLRDLEELASGDADKHGPLGFTRLFVSKPTIAAVAGYCV
                     AGGLELACWCDLRIADESAIFGCFERRFGVPLIDGGTQRLPRIVGLGRALELIMTGRP
                     VPAQQALAMGLANEVVAPGQCLPRALELAQSIAKFPQSCLRNDRQSVYTGLGHSLADG
                     LRIEADYGAQTLRSGESASGAAAFQAGKGRKGGSLTNP"
BASE COUNT          818 a          931 c          963 g          729 t
ORIGIN      
        1 caggcgcaaa aggcattaga agtgcctacg agagaatgca tagggaatac gaggagaacc
       61 aatatgaaag cgaagagagc cactgcacaa aatcccaggg cgaccgcaag ggtcgccctc
      121 gtcttcctgc tcaggtagct agatgaattc gccaggcttc aaagagaaag tcaggctgcg
      181 cggcatttgc ggagaatggc gatgaccttc ccctggacct gccattcgct gtcccactct
      241 tttctcggga tatagatggg acccatttcc gaattggctg gctgcagtcg aacgcgatta
      301 tgatcggtct cctggtaaaa gcgtttgagg gtggcgcgac cattaccctg catatggacg
      361 gcaacgacga tatcgccatt ctcgcaggtg gattgcggct tgatcaccac ataatccttg
      421 tcgcagatat ggtcttcgat catcgactgg cccttgacga ccagggcata cgcgccgggc
      481 tgctcgaatt ctcgcccgac ttccagcggc tcaacggggt cgctaaatac ctcgattggc
      541 tcgcctgcgg cgatactgcc catgacgtga atgccgaaag gattggtcct gagtttgatg
      601 ccccggctct tcttcgactc gcggtcgatc agccctttct tctcaagcat cgagagatga
      661 tagtctacat gtcccgtgct ggcgattttc atggcgctgc cgatttcgcg attggttggc
      721 ggcatgcctt cctcgcggat atactcaacg atgaagtcga agatgcgctt ctgaatgtca
      781 ctggtatggt ctgttcccat ttcttgctac ctgcctttct gaagaggcaa ttcagggtac
      841 tcttgccccg ttatactgtt tgagagtaga acagctattc aaattatact ggctggccta
      901 tcataaagtc aagcgttttg gagcgaatat tagaaaatat attctggttc gtctcatcac
      961 cggtaatgtt atgatgtcaa gcttgtcgta tcaggaagaa aaagtgctac tgaccaaaaa
     1021 aggacttgga atagtaacgg cgatttgcta tactcctgta aaagggatat ggagaagggc
     1081 atgctcccga cgatttgcgt tgtcctattg gaaggatgta caataagggc gtcttacacc
     1141 ggcgaaccca gccgccaaca gagggagtca gagagactat gatcgagatg acagttgaaa
     1201 gcgtgcgaat caatttgcag aactcgcagc gagtggtcat cttaaaagct gagcaacacg
     1261 aacgctacct gttcatctgg atagctcctg ccgaagccta tgctatcgcc atcgagctac
     1321 agggaacgag ttcgccgcgc ccacttacgc acgacctcat gaaaaacata atcaatgacc
     1381 tgggggcaac cattaccagt gtcgtgattt cagatttgat caacgacatc tattacgctc
     1441 gtatcgtgct cgatgtcgcc ggtcggcatg ttgaagttga ctcgcggcca agcgatgcga
     1501 tagccctggc cgtgcgcgct cacacaccaa tttatgtcga agagagcgtg ctggagcggg
     1561 cgggcgttgc gctagaaacc aatggcgaca cggtcacgcc aaagattgac cagcataaag
     1621 atcgagagat cgagtatgat aatctcgatg cttatcgaga ctttattaac agcctggacg
     1681 cattagacga gtttgggaaa gattaaaaga ggatggaagc aagggaacgc ctgatacgcc
     1741 aactgttgct gaagcagcgc tgtcaactct gccactcggt gtatacgacg gaaggaaatg
     1801 ttatcctagc ccgccgccgt gatgtgtggc tagtgatggc aagttgtgcc ttttgccagc
     1861 aaaaagatac cttcgttatc cagttctccg cgccgatccg gggccgtcgc cgcatcacca
     1921 gctatcgcct ctccaatccc gccacttcca atacaccctc aggcgcgcca cgaacgcagt
     1981 cacatccctc gcgtcaagcc aaacggccta tctcatccaa tgacgttcag gagatgcgtg
     2041 agtttctcac tcgattcgat ggggattttc agcggctttt tgcagaagtt gagtagcgcc
     2101 gatcagttag ttaacgcgaa ttgagcagcg ccttcaagaa ccgcaccagg atacgcgaga
     2161 tcaacccata gaccaggatg gccgccagcg gagccaggta attcaccacc cccggtccga
     2221 gctgcgagag cagttgaatt tgatccagca gcaggcgaaa cggcagggcg aagacgccac
     2281 cagcagcata gaggactttc gcccagagca gactgctact cacattgaat aaaaggagaa
     2341 tcacgcgcac cagcaggaag atctgcacgg cgacgaaaca tactccgacc acggtcggag
     2401 cggggctgcg gcgcggttgc gcgggtacac ccggcagcga gggcaggacc gggttatcca
     2461 gcggcgtttc cggcgcagat tggaagggat gaaagaccgg agacagcgcc gctgtcggct
     2521 ccgcagcatg ggcagcgtct gtcgggtcgg gcgaatgagg cgcgaggatt accggtatca
     2581 tcggatcggt cggcgaatca aaatcgttct cattcctgga ttggtcgtca aacgcgctgt
     2641 acatagcgtt ttgctgttcc gagattgtgc gtaacacgga tgcctcttac ttcctctcgc
     2701 tttggtttca cttatataat acgacatcat tgagaatatt taacattttg caacatgacg
     2761 ggttgtaaat acgtgaccaa acaggaatgg taataaaacc cgtaggagcc gataaatcgg
     2821 cggtgagcgc gatcaatcgg ctcctacggg tttgttaaac taccgccctt gcgacccttg
     2881 ccagcctgaa atgctgccgc gcccgacgcc gattccccac tgcgcaatgt ctgcgcgcca
     2941 tagtcggcct cgatgcgcaa accatcggcc agggaatgcc ccaggccggt atagaccgat
     3001 tggcggtcgt tgcgcaggca cgactgggga aatttggcga tggactgcgc aagttccagg
     3061 gcgcgtggca ggcactgacc gggggcaacg acttcgttgg ccaggcccat cgccagtgct
     3121 tgctgggcgg gaacgggccg accggtcata atcagttcca gggcgcggcc aagcccgacg
     3181 atacgcggca ggcgctgtgt gccgccgtcg atgagcggca caccgaaacg acgctcgaag
     3241 cagccgaaaa tagcgctttc gtccgctatc cgcagatcgc accagcaggc cagttctagc
     3301 ccgccagcaa cgcaatagcc cgcgacggcg gcgatggtgg gtttactcac aaacaggcgc
     3361 gtgaagccga gcgggccatg tttgtcggca tcgccgctcg ccagctcctc caggtcgcgc
     3421 aggtcggccc cggcgcagaa g
//
LOCUS       DASZAK010000088         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_641298, whole genome shotgun sequence.
ACCESSION   DASZAK010000088 DASZAK010000000
VERSION     DASZAK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641298"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="VGT44_01420"
     CDS             <1..433
                     /locus_tag="VGT44_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579484.1"
                     /translation="YATDISTAALDVAYLNCQRHHVEYRVRLLPGDLLAPLPEPIDIL
                     TANLPYVGTGEMDDLPPDVREYEPHLALFSGPQGLDLMARFFLELKQSQKLTEQAVIL
                     LEIGYRQREPLTTMLHEIWPQANITFTKDYAGWDRLLQLTL"
     gene            777..1931
                     /locus_tag="VGT44_01425"
     CDS             777..1931
                     /locus_tag="VGT44_01425"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEV2579485.1"
                     /translation="MGGVAMFLAFAIVSLLFYTPGSNLNAHANELTIYWLLIAAGALM
                     VLVHAYDDILGLGPWPKLLAQTIAVVIILGPWIDKTFHGILLFTFNNPFGVEHFNPNL
                     PWYREPTLFLFVQNSDITLAAIPAILLTWFWTVGMMNTVNWIDGMDGLATGVVGITAL
                     FITVISFMLHQYSIAVLAAIFTGAVFGFLPHNWNPAKIFMGDTGSMFLGLALAVLSIM
                     GGAKLALALMVLGVPILDVAVVIMNRIRRGQSPVHYDKTHLHHRLMATGLSVKQICYL
                     FYGVAITFGLLALSFSGIHAAHFYKFVGIILVILTMFGLIMWVDYRQRLRGVPIKLGG
                     PDTSPNGANEQGPQRSNSKSLREEPPESTTQASPPQSHSTDVHLRTPLLQ"
     gene            complement(1873..>2811)
                     /locus_tag="VGT44_01430"
     CDS             complement(1873..>2811)
                     /locus_tag="VGT44_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2579486.1"
                     /translation="KSSLITDILYRKLAHVLYRAHDKPGAHDAIEGIEFLDKVIDIDQ
                     SPIGRTPRSNPATYTNAFTGIRELFAQVPESRIRGYLPGRFSFNVKGGRCEACKGEGI
                     VKIEMNFLPDVYVPCEVCKGKRYNREALEIHYKGKNISDVLDMTVEEAMGFFANVPSI
                     YNKMKTLNDVGLGYMRLGQPATTLSGGEAQRVKLATELARRATGRTMYILDEPTTGLH
                     FADVDRLLEVLQRLVDAGNSIVVIEHNMEVVKCADWIIDLGPEGGDAGGEVIAQGIPE
                     DVAESEQSFTGYFLKRMFTEEAAFANARQSSEIVAG"
BASE COUNT          627 a          823 c          654 g          707 t
ORIGIN      
        1 ctacgccact gatatctcca ccgctgccct cgacgttgca tatctcaatt gccaaagaca
       61 ccatgttgaa taccgtgtgc gcctgctgcc tggtgatctc cttgcacccc tgcccgaacc
      121 catcgacata ctcactgcca acctgcctta cgtcggaacc ggtgagatgg atgacctgcc
      181 ccccgacgtt cgcgaatacg aaccccatct cgcgctcttc agcggtccac aaggattaga
      241 cctcatggcc cgtttttttc tcgaattgaa gcagtcacag aagctgacag aacaagccgt
      301 aatattatta gagatcggct atcgccagcg cgagccgctt accaccatgt tgcacgaaat
      361 ctggccgcaa gcaaacataa cattcacaaa ggactatgcc ggttgggacc gtctgttaca
      421 gctaacactc taattcgtat agagaagaaa gcagctagaa atcgcgtttt cttttacaga
      481 caccctataa tcgaggcatt ctctcaatgc cggtactctt tacaagaaga gaggaatgcg
      541 ctaagctaca taagtaaggg gatttgtttg ttatgctggg aggaagtatc atacatattc
      601 ggcttctatt gctcattccc atcaatcttc ccgacgtgat caccttgttg atcggcggaa
      661 tatccgcatt tgtgcttgcc tttgtactta catatttggt aaaagcattc tgctataaag
      721 taggatggct cgacaaaccg gccgcgcgca gagtccacac caaagctgtc cctcgtttgg
      781 gcggcgttgc tatgtttctg gccttcgcca ttgtctcatt gctcttttat accccaggct
      841 ctaacctcaa tgctcacgcc aacgaactca ctatttactg gctactcatt gccgcgggag
      901 ccttgatggt gctcgtgcat gcatatgatg acatcttggg gcttggtccc tggcccaaac
      961 tccttgctca gaccatagcg gttgtgatca ttcttggccc atggatagat aagacatttc
     1021 acggcatcct gcttttcacc ttcaacaatc ctttcggcgt cgaacatttc aatcccaact
     1081 tgccctggta ccgtgaacct acactctttc tgttcgtgca gaatagcgac atcaccctcg
     1141 ctgctattcc cgccatcttg cttacctggt tttggaccgt tggcatgatg aacaccgtga
     1201 actggattga cggcatggat ggcttagcaa caggcgttgt cggcattacc gcgcttttca
     1261 tcaccgtgat tagcttcatg ctgcaccagt atagcatcgc agtccttgcc gccattttca
     1321 ccggcgccgt ctttggtttt ctgcctcata actggaatcc tgcgaaaatt ttcatgggag
     1381 ataccggctc gatgtttctt ggactcgcgc tggcagttct ttcaattatg gggggagcga
     1441 agctcgcgct tgccttgatg gtgctcggcg ttcctatcct tgacgtggcc gtggtcatca
     1501 tgaaccgtat ccggcgtggt caatcgcccg tgcattacga caaaacgcac ctgcaccatc
     1561 gactgatggc aaccggactg agcgtcaaac aaatctgtta cctgttctac ggggtggcta
     1621 tcacgttcgg cttgctggcg ctcagtttct cggggataca cgcggcacac ttctacaaat
     1681 ttgtaggcat catactggtc atactcacga tgtttggctt aattatgtgg gtcgattatc
     1741 gccagcgcct gaggggtgtg ccaatcaaat taggcggtcc cgatacatca cccaacggcg
     1801 cgaatgagca aggtcctcaa cgttcaaact cgaagagcct gcgcgaagag ccacctgaat
     1861 ccacaacaca ggctagcccg ccacaatctc actcgactga cgtgcatttg cgaacgccgc
     1921 ttcttcagtg aacatgcgct tcaaaaagta gcccgtaaac gactgctcgc tctcggcgac
     1981 atcctccggt attccctggg caatgacttc cccaccggca tcgccaccct ccggccctaa
     2041 atcgataatc caatcggcgc acttgaccac ttccatgtta tgctcgatca ccactatcga
     2101 attacccgcg tcgacgagac gctgcaagac ctccagcagg cgatccacgt cggcaaagtg
     2161 cagtccggtc gttggctcat ccagaatata catcgtccga cccgtggctc gacgtgcaag
     2221 ctccgtcgcc agtttgacac gctgcgcctc gccacctgaa agcgtggtag ctggctgtcc
     2281 caggcgcata taacccagac cgacatcgtt caatgtcttc atcttattgt aaatcgaggg
     2341 cacattcgcg aaaaagccca tcgcttcttc caccgtcata tccagcacat cggaaatgtt
     2401 cttccctttg taatgaatct ccagcgcctc gcgattgtag cgcttgccct tacatacttc
     2461 gcatggcacg tacacatccg gcaggaagtt catctcgatt ttgacgatac cctcaccttt
     2521 acaggcttcg caacgcccac ctttcacatt gaacgaaaac cggccgggca ggtaaccacg
     2581 aatgcgggat tccggtacct gcgcgaagag ctcgcggatg cccgtgaaag cattggtata
     2641 ggtggctgga ttggagcgcg gcgtgcgtcc aatcggtgac tgatcgatat cgatcacctt
     2701 gtccagaaac tctatcccct cgatcgcatc gtgcgcccct ggcttgtcat gggcgcgata
     2761 cagcacgtga gccagtttcc ggtagaggat gtcggtgatg agcgagcttt t
//
LOCUS       DASZAK010000089         2942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_285915, whole genome shotgun sequence.
ACCESSION   DASZAK010000089 DASZAK010000000
VERSION     DASZAK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285915"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VGT44_01435"
     CDS             complement(<1..342)
                     /locus_tag="VGT44_01435"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579487.1"
                     /translation="MSLETTIPDVSDAQKTQETPPTRLFKNRNFGLLAIGQAISIIGD
                     FVYSTTLLVWVFVLTHSAAAVSGVLVAQYVPIFLLGPVAGVFVDRWNRRTTMIATTLL
                     QMGVALLPLLIP"
     gene            complement(347..1219)
                     /locus_tag="VGT44_01440"
     CDS             complement(347..1219)
                     /locus_tag="VGT44_01440"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2579488.1"
                     /translation="MIEHYEIETIEQLRAIADVLRLRILDALIPQPMTVTQLGDVLGM
                     APAKVHYHVRELERVGLLRLVETREKGGILEKYYQPIARYIGVEQSLLSAPANEAQAL
                     VSALLGQVHNNFLRAFRQGLEKRSEKPDLMLGLGISRLFITRDELKDLDKRIQELFKP
                     YETERHIEGEQEIFTMMLTYPENESMAQVAAAAATSTSPLSLVEGDADRVVGTARYSR
                     ADLEKALAEGKRLTITVVGVCQFADDVSVELADEAIEHLNVVGKLLASPRLREVLLRK
                     RSDQSATQVNRPED"
     gene            1420..1548
                     /locus_tag="VGT44_01445"
     CDS             1420..1548
                     /locus_tag="VGT44_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579489.1"
                     /translation="MQNIAADMHINGVFIWAWSFPPFAPNGKPAAQVLRQWFSMIA"
     gene            complement(1558..>2942)
                     /locus_tag="VGT44_01450"
     CDS             complement(1558..>2942)
                     /locus_tag="VGT44_01450"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2579490.1"
                     /translation="SRHVALRADLGLFLFVYGCVLAVSILITGNTGWAPLQVYTTIAW
                     TLYFPIAMIGIVGAFAGRRLPRLKYRGSIAQMVIFMIPSVVRDDTLPALIGVIDSVFA
                     SAPKQIPNFMIQIVVDEGAPALEKLAQHYREIDPNDQKHLWYTIVPKAYKTKHGTLKK
                     ARANHYAMLERARLGLERSDIFIFHLDDDTRITPQTAMTIADFVAHKSHKYYAAQGAL
                     AFPFQLTRSWFCAMMDSVRGGDDLLRFQAALLRFGGPIFGFHGENLLVNSVIEARIGW
                     DFGPVYVEDAYFALAFMRAYPRRATFLQSCTYGGSPETVGDMLIQRRRWSHGLFGLLA
                     DRKIPWSRKWMMGYAIINWALGLFQHAGVIFLLAALLGRLNTSPVASAFIFIWGFNLA
                     YQIWMYLTGLAINLSASQAAAWKYYVFPWLVVLLLPVFSFIEALAAILGFFDFLRGSK
                     EFRVIKKSVS"
BASE COUNT          703 a          868 c          773 g          598 t
ORIGIN      
        1 ggggatgagc aggggcagca gggccacgcc catttgtagt aaggtggtcg cgatcatcgt
       61 cgtgcgacga ttccagcgat ccacgaagac gcccgccacc ggcccgagca gaaaaatcgg
      121 cacatactgc gcaaccagta cgccgctcac cgccgccgcc gaatgtgtca gcacaaagac
      181 ccagacgagc agggtggtgc tatacacaaa atcgccgatg atcgaaatgg cctgaccaat
      241 agcgagcaag ccgaagttac gattcttgaa tagtctggta ggaggcgtct cctgcgtttt
      301 ctgggcatcc gagacatcgg gaatggtcgt ttcgagagac attacttcaa tcctctggtc
      361 gattgacctg ggtggccgac tggtcgctgc gtttcctcaa cagcacctcg cgcaaccggg
      421 gtgaagcgag aagcttgcca acgacattca gatgttcaat ggcctcatcc gccagttcca
      481 cgctgacatc atcagcgaac tggcacacgc ctaccaccgt gatagtgagc cgcttcccct
      541 ccgccagcgc cttctcaaga tcggcgcggc tgtaacgcgc cgtgccaacc acccggtccg
      601 cgtccccctc caccagggac aatggcgacg ttgaggttgc tgcggcagct gctacttgag
      661 ccatcgactc gttttcagga tacgtcagca tcatcgtaaa gatttcctgc tccccttcaa
      721 tatgacgctc agtctcatag ggcttaaaca gttcttgtat gcgcttgtcg aggtctttca
      781 actcatcacg tgtgatgaac aggcgcgaaa tgcccagacc caacatgaga tcaggtttct
      841 cgctgcgctt ctcaaggccc tggcggaagg cgcggagaaa gttattatgc acctgcccca
      901 gcaaggcgct cactaacgcc tgtgcctcgt tcgctggcgc ggatagtagt gattgctcaa
      961 cgccgatata acgggcaatc ggctggtagt atttttcgag aataccaccc ttttcgcgcg
     1021 tctccaccag cctcagcaac ccgacgcgct ccagttcgcg cacatgataa tgcaccttcg
     1081 ccggcgccat gcccaacaca tctcccagct gcgttaccgt catcggctgc ggtatcaggg
     1141 catcgaggat gcgcagccgc aacacatcgg caatcgcgcg caactgctcg atagtttcaa
     1201 tctcgtagtg ttcaatcata ttattctttt gatctttcaa atttttttga tcgcataaga
     1261 atagtatatc gagcaaagta ttttgtcaat gggaggtaaa ctgtcttcct ctcagagcta
     1321 gggtacagag atgcggcctt cgcgggctat aagccctggc taatcgaggc caacgattca
     1381 cgcgacgatg gcgagcaggc gctattattt gaggctgcct tgcagaatat cgccgccgat
     1441 atgcacatta acggcgtctt catctgggca tggagcttcc cacccttcgc gcccaatggc
     1501 aagcccgccg cccaggtctt acgccagtgg ttcagcatga tcgcatgatc gaggggccta
     1561 cgacacgctt ttcttaatca cgcgaaactc cttgctgccg cgcaagaagt cgaagaaacc
     1621 gagaatggcc gcgagcgcct cgataaagga gaagaccggc aggagcagca caaccagcca
     1681 gggaaagaca taatatttcc aggcagccgc ctgggaagcc gataaattga tggcgaggcc
     1741 ggtcaggtac atccagattt gataggccag gttaaagccc cagatgaaga taaaggccga
     1801 cgcgaccggg ctggtattga gtctcccaag cagcgccgcc agcaaaaaaa tcacgcctgc
     1861 atgttggaag aggcccaacg cccagttgat gatcgcatag cccatcatcc acttgcggct
     1921 ccagggtatc ttgcgatcgg ccagcaagcc aaacagccca tgactccaac gcctgcgctg
     1981 gatcagcata tcaccaaccg tttcgggcga gccgccgtag gtacatgact ggagaaaggt
     2041 ggcgcgcctg ggataggcgc gcataaaggc cagggcaaag taagcatctt ccacgtaaac
     2101 cggtccgaaa tcccagccaa ttctggcttc gatcacagaa ttgaccagca agttttcgcc
     2161 atgaaacccg aagatcgggc cgccgaagcg cagcagcgcc gcctgaaagc gcagcagatc
     2221 atccccgcct cgcaccgagt ccatcatggc gcagaaccat gagcgcgtca actgaaaggg
     2281 aaaagccagc gccccctgcg ccgcgtaata tttgtgcgac ttgtgcgcga caaaatcggc
     2341 gatggtcatg gcggtttgcg gcgtgatgcg cgtatcatca tccaggtgaa aaataaaaat
     2401 gtccgaacgc tcaagtccca gtcgggcgcg ttcgagcatc gcatagtggt tagccctggc
     2461 cttcttcagc gtcccatgct tcgtcttgta cgctttcggc acaattgtat accacagatg
     2521 tttctgatca ttcgggtcga tttcacgata gtgctgcgcc agtttttcca gcgccggcgc
     2581 gccttcatcc acaacgatct ggatcatgaa gttcggtatt tgcttcggcg ccgaagcaaa
     2641 taccgaatca atcacgccga tcaatgctgg cagggtgtca tccctcacca ccgagggaat
     2701 catgaagatg accatctgcg caatgcttcc ccgatatttc aagcggggca gacgcctgcc
     2761 cgcgaacgcg ccgacaatgc caatcatcgc gatggggaaa tacagcgtcc aggcaatcgt
     2821 tgtatagacc tgcagcggcg cccagcctgt attgccagta attaagatcg agacagccag
     2881 tacgcatcca tacacaaaga gaaacaggcc cagatctgcc cgtaaagcca cgtgacggct
     2941 ct
//
LOCUS       DASZAK010000090         2109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_570396, whole genome shotgun sequence.
ACCESSION   DASZAK010000090 DASZAK010000000
VERSION     DASZAK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570396"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VGT44_01455"
     CDS             complement(<1..412)
                     /locus_tag="VGT44_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579491.1"
                     /translation="MPLEQPQSIRTIFFDAGFTLIRPYPSTPEICQRICQQLGLHIHL
                     DTLKERMKGVDDLFFRMNRMRPQLWASDEAVNSFWIEYYMEYLRPFVEEHDERRLYEL
                     ALLINQEYDQHTSWQIYPDVLPTLEALHSRGYTLG"
     gene            complement(483..1703)
                     /locus_tag="VGT44_01460"
     CDS             complement(483..1703)
                     /locus_tag="VGT44_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEV2579492.1"
                     /translation="MAQMIMGSISIQENAIPEINPSYRLLLGPGPCNVDPRVLRVASA
                     PQLGHIDPEFFAMLEETALLLRHVFQTDNEMTLCISGSGFAGAEAVLSNLLEEGDTLI
                     AGSLGFFSGQIVEISERAGATVINLATELGKPLAPEQLEAAFQAHPEAKIFATAIAET
                     STGLWQPMEELERITHAHGALFVVDAVCGLGGIPLNVDAMRIDACYAGSQKSLGALPG
                     LAPVTLNKRAVSVIEQRKKPVQSYYLNLHKLNRYWNGDHTYHHTACSNVVYTMREALR
                     IALEEGLEARWKRHALHGEALRAGVKALGLSILTQPGYELPVLTAISLPDGVDEMAIR
                     NGLLEQYQIEIGGGFGALKGRLIRIGLMGYNSCRKNVDTVLSALEHVLPSSGLTPPPG
                     AALAAADAVYKTKG"
     gene            complement(1744..>2109)
                     /locus_tag="VGT44_01465"
     CDS             complement(1744..>2109)
                     /locus_tag="VGT44_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEV2579493.1"
                     /translation="VRITHLPTGLVVTIQDEKSQLKNKAKALAVLKSRLWDIEEAHRQ
                     EVLSKTRHSQVQTGDRSEKIRTYNYPQDRVTDHRISYTRHNLPGVLNGELDEFIDNLI
                     TVDQADKLQHLFEAEATAR"
BASE COUNT          379 a          627 c          643 g          460 t
ORIGIN      
        1 cgccgagggt gtaaccgcgc gaatgcagtg cctccagggt gggcagcaca tcggggtaga
       61 tctgccagct cgtgtgctgg tcgtattcct ggttgatgag cagcgccagt tcgtacagac
      121 gccgctcgtc gtgttcctcc acgaatggcc gcaggtactc catgtaatat tcgatccaga
      181 acgagttgac cgcctcatca ctggcccaca attgcggcct catgcggttc atgcgaaaga
      241 ataaatcgtc tacccccttc atgcgttctt tcagcgtgtc caggtgaatg tgcaggccga
      301 gctgctggca gatgcgctgg caaatctctg gagttgaggg ataggggcga atcagggtaa
      361 atccggcgtc aaagaagata gtgcggatcg actgcggttg ttcaagcggc atgtttcgtc
      421 cttctgaatg cgggtgatga gtcgctcctc gttcacaagc gtcccatcac ccgcatcatc
      481 gcttatcctt tcgtcttgta caccgcatct gctgcggcga gcgcggcgcc cggcggcggc
      541 gtgaggccgc tggagggcag cacgtgttcc agggcggaga ggacggtatc gacgtttttg
      601 cggcaggagt tatagcccat cagtccgatg cggatcaggc ggcctttcaa cgcgccaaag
      661 ccgccgccga tctcgatctg gtactgctca agcagcccgt tgcggatcgc catctcgtca
      721 acgccgtccg gcaggctgat ggcggtcagc acgggcaatt cgtagcccgg ctgggtcaga
      781 atggacaggc ccagcgcttt gacgcccgcg cgcaatgcct cgccgtgcag cgcgtggcgt
      841 ttccagcgcg cctccagccc ctcttccaac gcgatacgca gcgcctcgcg catggtgtaa
      901 actacgttgc tgcacgcggt atggtggtag gtgtgatcgc cgttccagta gcggttcagc
      961 ttgtgcaggt tgagatagta gctctgcacc ggcttcttgc gctgctcgat gacgctcacg
     1021 gcgcgcttgt tcagcgttac cggtgcgagg ccgggcagcg cacccaggct tttctggctg
     1081 ccagcgtagc atgcgtcaat gcgcatggcg tcgacgttga gcgggatgcc gcccaggccg
     1141 catacggcat cgacgacgaa aagcgcaccg tgcgcatggg taatacgttc cagttcttcc
     1201 atcggctgcc acaatccggt agaggtttcg gcgatggccg ttgcaaagat cttcgcctcc
     1261 gggtgcgcct gaaacgccgc ttcgagctgc tccggcgcaa gcggcttgcc cagttcggtt
     1321 gccaggttga tgaccgttgc cccggcgcgc tcggagattt cgacaatctg gccgctgaaa
     1381 aagcccaggc tgcccgcgat gagcgtatcg ccctcttcga gcaggttcga cagcaccgct
     1441 tccgcgcccg caaagccgga gccggagata cacagcgtca tctcgttgtc ggtctggaag
     1501 acgtgtctca agagcagcgc cgtttcttcc aacatggcga aaaattcggg gtcgatatgc
     1561 cccagctgcg gcgcggacgc cacacgcagc acacgcgggt caacattaca ggggccaggg
     1621 ccaagcagca ggcggtagga aggattgatt tcaggaatgg cattttcttg aatgctgatt
     1681 gatcccatta tcatctgagc catagaagga acatcccttt ctttgcaaac accctggtcc
     1741 ggcttacctg gccgttgcct ctgcttcaaa caggtgctgc aacttgtcgg cctgatcgac
     1801 agtgatcagg ttatcgatga actcgtccaa ctcgccgttg aggacgcccg gcaggttatg
     1861 gcgcgtgtag ctgatgcgat gatcggtgac acggtcctgc gggtagttgt aggtgcgtat
     1921 tttttcgctg cggtcgccgg tctgcacctg cgagtgccgc gtcttgctca atacttcctg
     1981 cctgtgcgcc tcttcgatat cccacaggcg cgatttcagc acggccagcg ccttcgcctt
     2041 gttcttcaac tgcgatttct catcctggat ggtcacgacc aggccggtcg gcaggtgggt
     2101 gatgcgcac
//
LOCUS       DASZAK010000091         2037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_285975, whole genome shotgun sequence.
ACCESSION   DASZAK010000091 DASZAK010000000
VERSION     DASZAK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285975"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(340..963)
                     /locus_tag="VGT44_01470"
     CDS             complement(340..963)
                     /locus_tag="VGT44_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV2579494.1"
                     /translation="MTVEQDLPDLRRLVVARFEQSMSVPGAFFTTEAERIAEACWAMA
                     RRFFQGGRLLAFGNGAWATDAQHVSVEFVHPVIVGKRALPALALTNDSATLSALLAGE
                     DAVMSFARQLKVLARPQDMAMGFSPDGRCANVIAALTTARQMGLLTLALVGGDGGLLG
                     QMGQNELDFCFVVQAHDPLVIQETHETLYHILWELVHIFFEHEGLLK"
     gene            complement(960..1232)
                     /locus_tag="VGT44_01475"
     CDS             complement(960..1232)
                     /locus_tag="VGT44_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEV2579495.1"
                     /translation="MCLGIPGQIVEMVDDDNHIAKVNVSGVKRNVNIMLVRPDGIVPG
                     DWVLIHVGFAMSKIDEAEAHETMKALQLMGDVYTDELKMLHQSQIE"
     gene            complement(1261..>2037)
                     /locus_tag="VGT44_01480"
     CDS             complement(1261..>2037)
                     /locus_tag="VGT44_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEV2579496.1"
                     /translation="GAVWGCEIMEASLLGFERLAHLTYVPLPGGEQAVRQPWRVAAAY
                     LAQSYGDAFLELDIPFVRRLDRSKWRALAQMIAKSINSPPTSSLGRLFDAVSALIGLR
                     SEVLYEGQAAIELEMLARSCGKVDTYPFGIGERTTAYSQGMLLQVEPMIRAIVSDIQQ
                     GQSTTQIASRFHRTIAELLATACHQAREQTGLNEVALSGGVFQNQQLLVQLMARLDEM
                     GFQVYVNRRVPPNDGGLSLGQLAVAASICRNNLKNQQFLD"
BASE COUNT          460 a          617 c          492 g          468 t
ORIGIN      
        1 aatcatgcat cattcgtctc gctgctcata ggttagcaat tgccacgcct ccgtgtacca
       61 gaagcaaatc gcccgggtct acactttcca caagcgttat atcaatctct tccgtcgtat
      121 atttctctgc cacaatctcc acaagcgcta tccctgtctc ctgatcgaca cgtagtacgc
      181 gagcggtcaa tgcttcgtct gaacatgtta cacagtgtcc ttctgcatca agttcacaag
      241 tcgcatcacc gccaggcgtc aattcatgtc tcgaggaatc gtctggatgg gattgggtga
      301 gggtaaacct cacccctacg ggctcgcgtt gcttgtcact catttcaata atccctcgtg
      361 ttcaaagaaa atatgcacca gttcccataa gatatggtag agcgtctcat gtgtttcctg
      421 gattaccaat gggtcgtgcg cctggacaac gaaacagaaa tcaagttcgt tctgccccat
      481 ttgtccaagc aacccgccat caccacctac cagagccagt gtgagcaaac ccatttgcct
      541 ggctgtggtc aacgctgcta tgacattcgc gcaccgtcca tccggtgaga aacccattgc
      601 catgtcctgt ggccgcgcca gcactttgag ctggcgtgcg aaagacatga ctgcatcttc
      661 tcctgccagc agagcgctga gtgtagcgct atcgttggtc agggccagcg ctgggagcgc
      721 tcgtttgccg acgatcaccg ggtgtacaaa ctcgaccgag acatgttgag cgtcggtcgc
      781 ccaggcgcca ttgccaaaag cgagcaaacg tcctccttga aagaagcgac gtgccatcgc
      841 ccagcaagcc tcagcgatgc gctctgcctc ggttgtgaag aacgccccgg gtacggacat
      901 gctctgctcg aagcgtgcca cgacgagcct acgcaagtcg ggcaggtcct gctctactgt
      961 cattcaatct ggctctgatg gagcattttg agctcatcgg tatagacatc acccataagt
     1021 tgcagtgctt tcatggtctc gtgcgcctcg gcctcgtcga tcttgctcat ggcaaatccg
     1081 acatggatca acacccaatc accagggaca ataccatcgg gacgaacaag catgatgttc
     1141 acgttacgct taacaccaga cacattaact ttggcaatat gattatcatc atccaccatt
     1201 tctacgatct gacccgggat accaaggcac atactctcct cctttataga acctgtcttg
     1261 ctaatccagg aactgctgat tcttcaggtt attcctacat atgcttgcag ccactgctag
     1321 ctggcccagg cttaaacccc catcattggg tggcactcgt cgatttacat agacctggaa
     1381 gcccatttcg tccagacgcg ccatcaattg caccagtaac tgttggttct ggaaaacacc
     1441 tccgctgagc gcgacttcgt tcaggccggt ttgctcgcgt gcctggtggc aggcagttgc
     1501 cagcaactct gcaatcgtgc gatgaaagcg acttgcgatc tgagtggtcg actgaccctg
     1561 ctggatatca ctcacgatgg cacgaatcat cggctccacc tgtagaagca taccctgtga
     1621 atacgctgta gttcgctctc caatgccgaa cgggtaggta tccactttcc cacaacttcg
     1681 agccagcatc tccaattcga tggctgcctg accctcgtag agcacttcgc tgcgcaggcc
     1741 gatcagcgcg gataccgcat caaacaatcg gcccaggcta gaagtcggtg gactattgat
     1801 acttttcgca atcatctgcg ccagcgcgcg ccacttcgag cggtctagcc ggcgcacaaa
     1861 aggaatgtcc agttccagaa atgcgtcgcc ataggactga gccaggtagg cggcggccac
     1921 gcgccagggc tgtcgcaccg cctgctcacc tccggggagc ggtacatagg tcaggtgagc
     1981 tagcctttca aaccccagca gggaagcctc catgatctcg cagccccata cggctcc
//
LOCUS       DASZAK010000092         3352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_392745, whole genome shotgun sequence.
ACCESSION   DASZAK010000092 DASZAK010000000
VERSION     DASZAK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392745"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2441
                     /locus_tag="VGT44_01485"
     CDS             <1..2441
                     /locus_tag="VGT44_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2579497.1"
                     /translation="GGVGKTSLATRVAAMLLSEFRDGVYFIPLVPVLDPALLAPTIAN
                     VFGIGENGGKPLLRQLKSALREKHVLLLLDNFEQVLAAAPELSSLLSDCPGLNMIVTS
                     RAVLRVRGEHEFAVHPLAVPDLKHLPGDDTLPRFAAIALFLQCARTARPDFQPAPASM
                     RAIAEICVRLDGLPLAIELAAARIKLLPPLALLARLERRLEVLTGGTRDAPLRQRTLR
                     NTIGWSYDLLAPGQQALFRRLSVFVSGCTLEAVETLYSALDEGTLQVLDGVALLIDES
                     LLQQQEYEAAPRLVMLETIREFGLEMLAASGEWERVSDAHARCYLQLAEDAETEIGGP
                     AQANWLRRLEQEHDNVRAAMLWLLEKGEKQSDMSLALRLCGALQTFWVVHGHISEGRK
                     FLERALAASEGIEAVVLAKALITATYLAFVQSDFAYAEPLCKHGLALYQELEDQPGIA
                     FALSLLGSIAWTNGDMAQARALTEESLGISRQIDFTERAARALFILGLVDSSQGEYAR
                     AREHYEESLEIYRAMGNKRGAAHTLSQLAQVLLVEQAEAQQISQLLDECLALSHEIGF
                     KEGIAAYQCVSAQIALIRGDLDAAQKLAEQSVALYQEMEHKHGAAKSLSVQAKVHALK
                     GEFAAAQSCYEQSLAITGKLSEQWVASVYLMELGEVVAEQRQLAWAAQLWGTAESLRE
                     TYHVPIHLTERADYERSVAFARAHLGERAFAAAWAQGRAMTTEQALAAKGQKPAREAA
                     ATATLATYPDGLTAREVEVLRLVAGGLTDAQVAEQLVLSPRTVHAHLSSTYNKIGVTS
                     RSAATRYAIEHRLA"
     gene            complement(2458..3015)
                     /locus_tag="VGT44_01490"
     CDS             complement(2458..3015)
                     /locus_tag="VGT44_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579498.1"
                     /translation="MVSPEHIDNHKPEDQQAPSPGVPHQVPVVVEKKRTWLKPLMRVA
                     ALVLSLGGSFAGFWAFFSIQLQQGNNGEGWAILGAILLGTVSAVLFRSWWALVVIPIA
                     FSAGAFLAIYLMPLVISPNPLAVDDVGFGAFLWAVFGPIFAVFGALLGILILKTLEPN
                     QKNEQELLKHPFPNTLLEYALAALP"
BASE COUNT          727 a          957 c          968 g          700 t
ORIGIN      
        1 ccggtggcgt cggtaaaacc agcctggcga cgcgggtggc tgccatgctg ttgagcgagt
       61 ttcgcgacgg cgtctatttc attcccctcg tcccggtgct tgaccctgcg ctgctggcgc
      121 cgacaatcgc caatgtattt ggcattggcg aaaatggtgg aaaaccgctg ctgcgccagt
      181 tgaaatccgc actacgcgaa aaacatgtcc ttctcctcct ggataacttt gaacaagtgc
      241 tggcggcagc gcccgaactc tccagcctgt tgagtgactg tcctggactg aatatgatcg
      301 tgacgagtcg tgctgtgctg cgcgtgcgcg gtgagcatga gttcgcggtg catcccctgg
      361 cggtgccgga cttgaagcat ctgcccggcg acgatacact gccgcgcttt gctgcaattg
      421 ccctcttcct gcaatgcgcg cggaccgcca ggccggattt tcagcctgca cccgccagta
      481 tgcgggccat cgcagagatt tgtgtgcgcc tggatgggtt gcccctggcg atcgaacttg
      541 cggcagcgcg tatcaagctg ctgcctccac tagcattgct ggcgcgcctc gaacggcgat
      601 tagaggtgtt aaccggcgga acacgcgatg ctcctttgcg ccagcggaca ctacgcaata
      661 ccatcggatg gagctatgac ctgttggcgc ctggccagca ggcactcttc cggcgtcttt
      721 ctgtcttcgt gagcggctgt acgttggagg cagtcgaaac gctctatagc gcgctcgacg
      781 agggtacatt gcaggtgctg gacggcgtcg cattattgat tgatgaaagc ctgctgcaac
      841 agcaggaata tgaggccgcg ccgcgcctgg tcatgctgga aaccatccgc gaatttggat
      901 tggagatgct ggccgcgagt ggagagtggg agcgcgtcag cgatgcccac gctcgctgct
      961 atttgcaatt ggcggaagac gcggagacgg aaatcggtgg cccggcgcag gcgaactggt
     1021 tgagacgcct ggagcaggag catgataatg tgcgcgcagc tatgctctgg ctgcttgaaa
     1081 agggcgaaaa acaaagcgat atgtcgttgg ctctgcgctt gtgcggcgca ttgcaaacgt
     1141 tctgggtagt gcatggccat atcagcgaag gtcgcaagtt tcttgagcgg gcattggcgg
     1201 ccagcgaggg catcgaggcg gttgtgctgg cgaaggctct tatcactgcc acatatctcg
     1261 cttttgtgca gagcgatttt gcgtatgcgg aaccgttatg caaacatggg ctggcgctct
     1321 accaggaact cgaagaccag ccgggcattg ccttcgccct ttcgctgttg ggaagcatcg
     1381 cctggacaaa cggtgatatg gcccaggcgc gcgcgctgac agaagaatcg ctggggattt
     1441 ccaggcaaat agattttact gagcgcgccg cgcgagcgct gtttattctg ggcctggttg
     1501 acagtagcca gggcgaatat gccagagcca gggagcatta cgaagagagc ctggaaatct
     1561 accgggcgat gggcaacaaa cgaggcgctg ctcatacgct ctcccagtta gcgcaggtgc
     1621 ttctggttga gcaggcggaa gcgcagcaaa tatcccaact acttgatgaa tgcctggcgc
     1681 tttctcacga gattggcttc aaagagggca tcgcggccta tcaatgcgtc tcggcgcaaa
     1741 tcgcgcttat ccggggagac cttgacgccg cgcaaaagct ggccgaacag agtgtggctc
     1801 tgtaccagga aatggaacat aaacatggcg cggccaaatc gctctcggtt caggccaaag
     1861 tccacgcgct caaaggtgag ttcgcggcgg cgcagtcgtg ctacgagcaa agtctggcca
     1921 tcaccggcaa gttgagcgaa caatgggttg cctcggtcta tctgatggag ctgggcgagg
     1981 tggtcgccga gcagcgccag ctggcctggg ccgcacagct ctggggaaca gcagagagcc
     2041 tgcgcgaaac ctaccacgtg ccgatccatc tgaccgaacg cgccgactat gagcgctcgg
     2101 tcgccttcgc gcgcgcccac ctgggggagc gcgcctttgc cgccgcctgg gcgcaggggc
     2161 gcgccatgac gaccgaacag gcgcttgccg caaaggggca aaaacccgcc cgcgaagctg
     2221 ctgcaacggc taccctcgct acctaccctg atggcttgac tgctcgcgaa gtagaagttc
     2281 tgcgccttgt cgccgggggc ctgactgacg cgcaggtcgc cgaacaactg gtgctcagcc
     2341 cccgcaccgt tcatgcccac ctcagctcca cctacaacaa gatcggtgtt acctcgcgca
     2401 gtgccgccac gcgctatgcc atcgaacacc gcctcgcgtg aacgctcgtc accctcttca
     2461 aggtaatgca gctagtgcat attcaagcaa cgtgttcggg aaagggtgct tcaaaagctc
     2521 ttgctcattt ttttgattcg gttccagtgt tttgaggatg aggatgccca aaagggcccc
     2581 gaagacggca aaaataggac caaagaccgc ccacagaaaa gccccaaaac ccacatcatc
     2641 aactgcaagg gggttaggtg atatgacaag aggcatcagg tatattgcca gaaacgctcc
     2701 agcggagaat gctataggga tgaccactaa tgcccaccaa gagcggaaga gaacagcact
     2761 gaccgttcct aaaagtatcg ccccaagtat tgcccaaccc tccccattgt taccctgttg
     2821 aagttgaatg ctaaaaaagg cccagaatcc agcaaaactc cctcccagtg acaaaacaag
     2881 agctgctaca cgcatcagcg gcttcagcca cgttcgtttt ttctcaacta ccaccggtac
     2941 ctggtgtgga acaccagggg aaggcgcttg ttggtcttca ggcttgtgat tatcgatatg
     3001 ttctggcgat accatgatgc cacgctcctt tcacctcatc atgtggtggt gcgcttctac
     3061 aggaagaact tgttcctcat ctctagtgta tcgcgtgaat atcacaacgg tatctccgaa
     3121 gcctcgctca acttcacgat gtcgtcacag gttcaaaaag tcagcgcatt cccgaaaaag
     3181 tctcgtgagt gtgtactagg taattcagcc taacccactt ccatcttgct ccgcatctaa
     3241 attacacgat tctgacaatg tcttcccccc aggaataagg tatacctctc ttacaaggat
     3301 tgctgcgcat gtagtcccct cgtaggagcc ggtttaccgc gctcatcgcc ga
//
LOCUS       DASZAK010000093         4199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_357420, whole genome shotgun sequence.
ACCESSION   DASZAK010000093 DASZAK010000000
VERSION     DASZAK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4199
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357420"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VGT44_01495"
     CDS             <1..734
                     /locus_tag="VGT44_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2579499.1"
                     /translation="DMLKDQFIVTASHELRTPLTAVQGYIDLLQSYDDRLTPQARADF
                     IAKAGRGCDELTLMVNNIMDASRVQEEAVEVELSPITLAEPVAQVMEMMEAIVTRENR
                     QVSVSIAEDIVVKAEEQRLKQIILNLVGNALKYSPPGTPLEISACPDDGSSAEQAVCT
                     LRVRDYGSGVPLEEQQRLFERFVRLERDMNSPVRGAGLGLFICKQLAEAMGGSIWVES
                     TGVEGEGSSFYVAFKLHHPAQMPVR"
     gene            complement(697..1347)
                     /locus_tag="VGT44_01500"
     CDS             complement(697..1347)
                     /locus_tag="VGT44_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2579500.1"
                     /translation="MPEESRSVSTENNTVHEAPAQGVTEDFAQGVVSHLDSLYRTALR
                     LTHNTQEAEDLVQETMLKAFRFSTQYQPGTNLRAWLFRILNTSAINRYRKQSTHPAPA
                     SLPEGEEFYLYNRIRDLSGQELSKAAEDEVLSHYLDEDVFQALNELPINFRMPVILAD
                     IEGMSYKEIAETLQIPIGTVMSRISRARRQLQHLLWDYAKEKGYISVPASEPGDAA"
     gene            1508..1798
                     /locus_tag="VGT44_01505"
     CDS             1508..1798
                     /locus_tag="VGT44_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV2579501.1"
                     /translation="MDCIETIARLHLYVDHELTVDEVTIVQRHLVDCPHCECRFHFDM
                     SLKRLLHEHCAMERAPAHLREAVLRIASTPAGQQIDFDPELELRMRAELDDC"
     gene            complement(1828..2619)
                     /locus_tag="VGT44_01510"
     CDS             complement(1828..2619)
                     /locus_tag="VGT44_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEV2579502.1"
                     /translation="MISSNMTLGVAFLAGLASFLSPCVLPLVPIYLAQLVGQGVYQAA
                     ATVESTEVTMVSAASDAPSVAVGEFITRPARLNTFLHALLFVAGFTLAFVALGATAST
                     LGAFLKIHQFLLREIGGVALVIIGLHLTGILKLPFLYWQKRFEFHPERPGFLASFVIG
                     LIFAIGWTPCIGLILGPILALAANSETLREGVMLLLVYSLGLGIPFLLLGLGLDRLSR
                     LLKWLKPHTGKIEVGTGVIMIVMGVMIYFNLLLYLNRFFNIGITV"
     gene            complement(2698..3933)
                     /locus_tag="VGT44_01515"
     CDS             complement(2698..3933)
                     /locus_tag="VGT44_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2579503.1"
                     /translation="MNELAGQSLGGYLLEEEIGRGSMGMVYRGKQIALGREVAIKVLP
                     RALAKDASYVARFIREAQIIAGLNHPHIVHIYDAGQQGGVLYFVMEYVQGPTIGSLLQ
                     IDLIMPPHLAVEYAAEIADALDSAYKERNVIHRDIKPENLMLDRWGKIKIMDFGLARA
                     SGHQQITIAKTLVGSIYYASPEQVLGHQLDNRSDIYALGVVLYEMVTGQRPFNGRTLP
                     ELTQAIVNGSPRPPTELNPEVSLDLEHIILTAIARNRDDRYESASLMAQDLRGLGLLP
                     PVGSSIQSARVYGATGVPGTPRQPGQRDSFVVRLPKRPQAQRPRYLDLEDTQPPPGGQ
                     APEEQRLSPQPASPPQTPSLPIAEQTDEVPTRPLNSDQPERELSDVPVSGAEEDAISQ
                     EERRRRWRPFQRKPKSPEQ"
     gene            complement(4083..>4199)
                     /locus_tag="VGT44_01520"
     CDS             complement(4083..>4199)
                     /locus_tag="VGT44_01520"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEV2579504.1"
                     /translation="EVGKKLKVTRERVRQIEERAIRKLRHPQASRILKDYLD"
BASE COUNT          919 a         1145 c         1214 g          921 t
ORIGIN      
        1 tggacatgtt gaaagatcag ttcatcgtga ccgcctcgca cgaattacgt acccctctca
       61 cggcggtgca gggctacatc gatctgttgc agagttacga tgatcgcctg acccctcaag
      121 cccgcgccga cttcatcgcc aaagccgggc gcggctgcga tgaactgacg ttgatggtta
      181 acaatatcat ggatgccagc cgggtacagg aggaggccgt cgaggtagag ctatcgccga
      241 tcacgctggc ggaaccggtc gcccaggtga tggagatgat ggaagccatc gttacccgcg
      301 agaacaggca agtatcggtc tcaatcgcag aggatatcgt ggtgaaggca gaagagcagc
      361 gcttgaaaca gatcatcctg aacctggtgg gcaacgccct gaagtattcc cctcccggca
      421 caccactgga gatctcggcc tgcccggacg atggctcttc tgcggagcag gcggtgtgta
      481 cgctgcgcgt ccgcgactat ggctccggtg tgccgctcga agagcaacaa cgtctcttcg
      541 agcgttttgt gcggctggaa cgtgatatga acagccctgt gcgcggtgca ggattggggc
      601 tatttatctg caagcaactg gcagaggcca tggggggcag tatctgggta gaaagtaccg
      661 gcgtagaggg cgagggcagc agcttttatg tagcgttcaa gctgcatcac ccggctcaga
      721 tgccggtacg ctaatatagc ccttctcttt ggcataatcc cacaataagt gctgcaattg
      781 cctgcgagcg cgcgaaatgc ggctcataac ggtgccgatg ggaatctgca acgtctcggc
      841 aatttcttta taactcatac cctcgatgtc ggccaggatg accggcatac ggaagttgat
      901 cggcagttca ttcaacgcct ggaatacatc ctcatcgaga taatggctca gcacctcgtc
      961 ctcagccgcc tttgacagtt cttgcccgct caggtcacga atgcggttgt agagataaaa
     1021 ttcctcgcct tcgggcaatg aggccggagc cgggtgcgtg gactgcttgc gataacgatt
     1081 aatggcagag gtattcaaaa tgcggaagag ccaggcacgc agattcgtgc caggctgata
     1141 ctgcgtggag aagcggaatg ctttgagcat ggtttcttgt accaggtctt ccgcctcctg
     1201 tgtattgtgc gtcagcctca gcgccgtgcg atataaacta tctagatggg aaacgacacc
     1261 ctgggcaaaa tcctccgtta ctccttgggc aggtgcttca tgaaccgtat tattttcggt
     1321 cgataccgac cgggactctt ctggcatcaa aatctcctct atcgtcaggg tgacgcctcg
     1381 ctattgagcg aatgcaccct gttgcagtat aacgcattct gtttgggaat gaaagtgctt
     1441 ctcaattatg ttagaatgga cagaaataat ttgagcctgg agtagtagaa ggtctatgga
     1501 agaatgtatg gactgtattg aaaccattgc tcgtctgcat ctctatgtag atcacgagtt
     1561 gaccgtcgat gaagtcacga tcgtgcaacg acacctggta gattgccctc attgcgaatg
     1621 ccgctttcat ttcgatatga gtctcaagcg attgttgcat gaacattgcg ccatggaacg
     1681 cgcgcctgca cacttgcgcg aagcggtgct gcgtatcgcc agcactcctg ccggtcaaca
     1741 aatcgacttc gaccccgagt tagaactgcg aatgcgggcc gaattagatg attgctaatc
     1801 ctgtagacat cgccgtgtcc caggacgtta caccgtgatc ccaatgttaa agaaacgatt
     1861 gaggtagagc agcaggttga aatagatcat gacgcccatc acgatcataa tgacgcctgt
     1921 gccgacctca attttgcccg tgtgcggctt gagccatttc agcagcctgc tcaggcggtc
     1981 aagcccgagg cccagcagca aaaacgggat gcccagaccc agcgagtaga ccagcaacag
     2041 cattacgcct tcgcgcagtg tctccgagtt agccgccaac gccagaatcg gccccaggat
     2101 caggccgatg cagggtgtcc agccaatcgc gaagatcaac ccgatcacga aggaggccag
     2161 gaatccgggg cgttcggggt gaaactcgaa gcgcttttgc cagtacagga agggcaattt
     2221 caggatgccg gtcaggtgca acccgatgat aaccagcgcc actcccccga tctccctcaa
     2281 caagaactgg tgaattttca ggaatgcgcc aagtgtgctt gccgtggccc ccagcgccac
     2341 aaaagcgagc gtaaagcccg caacaaaaag cagcgcgtgt aaaaatgtat tgagtcgcgc
     2401 cgggcgcgtg atgaactcgc cgactgccac tgagggcgca tccgacgctg ctgacaccat
     2461 cgttacttcc gtactctcta ccgtcgccgc ggcttgatac actccctgtc caaccagttg
     2521 cgccaggtaa attggcacca gcggcagaac gcagggggag agaaacgagg caagtccagc
     2581 aagaaaagcc acgccgagtg tcatatttga tgaaatcatt accggcactg tctcccagac
     2641 ctgaaaatat attgctgcgt attactatag tacgaatatg ggtttcgcgc aagcagctta
     2701 ctgctcaggt gactttggtt tgcgttggaa aggccgccag cgtctgcgtc gctcttcctg
     2761 gctgatggcg tcttcctccg cgcctgagac aggcacgtcg ctcaattctc tttcgggctg
     2821 gtcgctgttc aaggggcgtg ttggcacttc atcggtctgc tcagcgatag gcagggaggg
     2881 cgtttgaggt gggctagccg gttggggcga caggcgctgt tcctccgggg cttgtccgcc
     2941 aggtggtggc tgagtatctt cgaggtcgag gtaacgcgga cgctgcgcct ggggacgttt
     3001 gggcaggcgc accacgaagc tgtcccgctg gcctggctga cgcggggttc cgggcacacc
     3061 ggtagcgcca tagacgcgcg cggactggat tgacgagccg accggtggaa gcaggcccag
     3121 cccgcgcagg tcttgcgcca tcaaagaggc gctttcgtag cggtcgtcgc ggttccgagc
     3181 gatagccgtc aggatgatgt gttccaggtc aagcgagact tctggattga gttctgtggg
     3241 cggacgcggc gaaccgttaa cgatggcctg cgtcagttcc ggcagagtgc gcccgttgaa
     3301 aggtcgctga cccgtcacca tttcgtagag gaccacgccg agcgcgtaga tatcgctgcg
     3361 gttgtcgagc tgatggccca ggacctgttc gggtgatgcg tagtagatac tgccgaccag
     3421 tgttttggca atggtgattt gttgatgccc ggaggcgcgc gccaggccaa aatccataat
     3481 tttgatcttg ccccagcggt ccagcatcaa gttttccggc ttgatatccc ggtgaatgac
     3541 gttgcgttct ttgtaggcgg agtcgagcgc gtcggctatc tcggcggcat attcaacggc
     3601 caggtgcggg ggcatgatca aatcgatttg caacagactg ccgatggtcg gtccctgcac
     3661 atactccatg acgaaataga ggacaccgcc ctgttgaccc gcgtcataga tgtggacgat
     3721 atggggatga ttcaagcctg cgatgatttg cgcctcgcgg atgaatcgcg cgacatacga
     3781 ggcgtctttg gcgagggcgc gtggcaaaac cttaatcgcc acctcgcgcc ccagagctat
     3841 ttgcttgccg cgatagacca tccccataga gccacgcccg atctcttcct ccaacagata
     3901 gccgcccagg ctctggcctg ccagttcgtt catacattca gcagcgcagg gaccccatgt
     3961 gagccatggg gagggagcgc tgtcctcctt ccctcaattt atgagattgg acgcattgac
     4021 cgatcttggt tagcataact tcggtcggct gcccaatcga tccacctcac tatcaataaa
     4081 catcaatcga gataatcttt gaggatgcga ctggcttgtg gatgacgcaa cttgcgaata
     4141 gcgcgctctt cgatctggcg cacacgctcg cgtgtgactt tgagcttctt tccgacctc
//
LOCUS       DASZAK010000094         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_109081, whole genome shotgun sequence.
ACCESSION   DASZAK010000094 DASZAK010000000
VERSION     DASZAK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109081"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(345..653)
                     /gene="cas2"
                     /locus_tag="VGT44_01525"
     CDS             complement(345..653)
                     /gene="cas2"
                     /locus_tag="VGT44_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEV2579505.1"
                     /translation="MSRRTRSRYSETSCYVIAYDIPNDKRRTKVHQILLGFGKWTQYS
                     LFECFLSKTELRLLQAKLAKHLVAREDSVRFYPLCANCVEKVETIGGPPPAEDLVYVV
                     "
     gene            complement(659..1756)
                     /gene="cas1d"
                     /locus_tag="VGT44_01530"
     CDS             complement(659..1756)
                     /gene="cas1d"
                     /locus_tag="VGT44_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-D CRISPR-associated endonuclease Cas1d"
                     /protein_id="HEV2579506.1"
                     /translation="MATLYLTEEYAAVRRETENMLRVEIPARQARDGVKAAPARSERI
                     PLIKIDEVVVLGEITLTAAALHLLLEHEIEITFLGHYGQFKGRLSPPFSKNAVLRMAQ
                     YRAHQDMAKRCELARRFVMGKLANQRQRLLRYNRAQADREVGQAIDQLAVALKTLEAL
                     PIPPVLPARTLAGGDHRVEGTPLETILGIEGAGSAAYFQCFGKLLTRPDHWPFPGRVK
                     RPPTDPVNSLLSFGYSLLTSKVASAVQLVGFDHFVGYLHSSFYGRPALALDLMEEFRP
                     VIVDSVVLNILNHRILTSNDFVVELGAYRLKQEKRTLFFTKLEERLNEEVNHPTFGYK
                     TTYRRCLELQVRLLAKVLTGEISEYPPFLAR"
BASE COUNT          417 a          614 c          545 g          424 t
ORIGIN      
        1 agagccgctc cgtgtgcaat ctcaacacct tgacctacct catcatcaat gccaacgcgc
       61 tcgctcgctg tcgctcaata aatacttcat tgctctcgcc gattcaactc agacaataga
      121 gccgaccctc tcaagcggga agccaggcgg ttcgcccgat gtgctcgtaa tatagcatca
      181 gaagccaaaa aaagcaagcg ttttcgcggg acaccttttt ggggggattt ttggatgcac
      241 taatcagggc aacctttaac ctgtatttcc tgccactcgg cttctagagc catgcgcggg
      301 tctacgctca gccggtcact tccggcgacc cgcgataagc tctgctaaac cacatagacc
      361 agatcctctg ctgggggagg accaccgatc gtctcgacct tctcgacaca gttggcgcat
      421 aaggggtaga agcgcacgct gtcctctctc gccaccaggt gtttggcgag cttagcctgt
      481 aacagccgca gttcggtctt gctcagaaag cattcaaaca acgaatactg cgtccacttg
      541 ccgaagccca acagaatttg atgcaccttc gtgcgccgct tatcattggg aatatcatac
      601 gcgatcacat aacaactggt ctcgctatag cggctgcgcg ttctcctgct catcgcgctt
      661 acctggcgag gaaaggcggg tactcgctga tttcccctgt caaaaccttg gcaagtagcc
      721 gcacttgcag ctcgagacaa cgccggtagg tcgttttgta gccaaaggtc gggtggttca
      781 cctcttcatt caagcgctct tccagcttgg taaagaacag cgtgcgtttc tcttgcttca
      841 gacgataggc ccccagctcg accacgaagt cattggaggt aaggatgcgg tggttcagga
      901 tattgagcac gaccgagtcc acaatcaccg gacggaattc ttccatgaga tctaacgcca
      961 gggccggacg cccatagaac gaactgtgca ggtagccgac aaagtgatca aagcccacta
     1021 gctgcaccgc gctggccacc ttgctggtca gcagcgagta cccgaagctc agcagggagt
     1081 tcaccggatc ggtcggaggg cgcttcaccc gcccagggaa gggccagtga tccggcctcg
     1141 tcagcagctt gccaaaacac tgaaagtagg ccgcgctgcc tgccccctcg atccccagga
     1201 tcgtttccag cggcgtgccc tccacgcggt gatctccgcc tgctaaggtg cgtgctggca
     1261 ggacgggcgg gatcggcagc gcttccaggg ttttcaaggc gacggcgagc tggtcaatgg
     1321 cctggcccac ttcccgatcc gcctgggcgc gattgtagcg cagtaagcgc tggcgctgat
     1381 tcgccagctt ccccatcacg aagcgccgtg ccagttcgca gcgcttcgcc atgtcctggt
     1441 gcgcccggta ctgggccatg cgcaggaccg cattcttgga gaacggaggc gacaggcggc
     1501 ccttgaactg tccatagtgt cctaaaaagg taatctcgat ctcgtgttcc agcaataaat
     1561 gcagcgccgc cgcggtcagc gtaatctcgc ccagcactac cacctcatcg atcttgatca
     1621 aggggatacg ctcgctgcgc gcgggcgcgg ccttgacgcc atccctggcc tgcctggccg
     1681 gaatctctac ccgcagcata ttttcggtct cgcggcgcac ggccgcatat tcttccgtca
     1741 agtacagggt tgccataata ctatcccttt cgcacgtgta gaggcgtaat gatgcttgtt
     1801 cacatccacc aacggcattg tcatcctgag cgcagcgaag gatctcggcg gccagggcat
     1861 cagatccttc gctgcgctca agatgacaat gccggcctgg tttgtaataa tttcacttct
     1921 tccggcagac agatgggcag caggctacac ggtgaacagc gtgccacgag tttccctgtg
     1981 agtggcggcg gcggggtctc
//
LOCUS       DASZAK010000095         2296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_357498, whole genome shotgun sequence.
ACCESSION   DASZAK010000095 DASZAK010000000
VERSION     DASZAK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357498"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VGT44_01535"
     CDS             <1..399
                     /locus_tag="VGT44_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2579507.1"
                     /translation="PRTTLASDIHCVTSWSRYDNTWEGVHIREILQRAKPLPSAGFVT
                     AHSWTGYTTNMPLADLDDDDVMIVIKHDGEDLAPEHGGPVRLLVPKLYFYKSAKWFDG
                     LEFMEHDRPGFWEQRGYHNHADPWKEERYW"
     gene            431..880
                     /locus_tag="VGT44_01540"
     CDS             431..880
                     /locus_tag="VGT44_01540"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEV2579508.1"
                     /translation="MFDITKIGYCFPPYPVTIERTKLRELALAIGDPDVVYQDRQAAH
                     AAGYEDVPLPPTTATILLFWENRHFLEQLVELGLDISRLIHREETYEYLAPLHPGETL
                     MGVMTVLDGATRKGPRGSAIDLITLQLRYTNREGQLALIATTRLVTQ"
     gene            complement(888..>2296)
                     /locus_tag="VGT44_01545"
     CDS             complement(888..>2296)
                     /locus_tag="VGT44_01545"
                     /inference="COORDINATES: protein motif:HMM:NF016201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEV2579509.1"
                     /translation="TPGNSGKPLYSRRFPLVAPTHVTEAFAEFLGYLVGDGNIHVTKN
                     AIGYTSGDRELADRYAALVRELFAIEAAPFWDAHTANGKGGRWRVVFYSANVLDLLQS
                     LGIDLHAKARDKRIPDCILRSPKAVVSAFLRAYFDCDGCASVKQGVILSTFSDEIART
                     LQILLLNYGILSRRRGVNVWISGKSAQLYAQEIGFGLARKQGNLQDYLASHRWFRLEE
                     PFDEVVSIEHDVADVYDITVDRTHRYVANGMLHHNSTWHSRIMRRLGETGAISDSDTI
                     EFAQLHSGVLSPSRTYLNPYYIGYKVFEDIERRWDNPTREEQERLGRKPGQGKAKIFE
                     VREMENDVSFLRNYLTEDLVKDLDLYLYRKEGDEWVIVEKNWEKVRDSIVTSMTNFGV
                     PYLVVDNGDYRGNRELYIKHLYEGQELDMVYAEKTLQHVYTLWGRPIHLETLYEGKRI
                     LLTYDGERNTKSSLEKEK"
BASE COUNT          460 a          622 c          700 g          514 t
ORIGIN      
        1 ccgcgcacta cactggcctc tgatattcac tgtgtgacgt cgtggagtcg ttatgacaat
       61 acgtgggagg gtgtgcacat ccgcgagatt ttacagcggg ccaaaccgtt gccgagcgcc
      121 gggtttgtga cggctcattc ctggacgggt tacacgacga atatgccgct tgccgacctg
      181 gatgacgatg atgtgatgat tgttatcaag catgacgggg aagacctggc tcctgagcat
      241 ggtggacctg tgcgcctgct tgtgccgaag ctctacttct ataagagcgc gaaatggttc
      301 gatggcctcg aattcatgga acacgaccgc cccggcttct gggagcagcg cggctatcat
      361 aaccatgccg acccgtggaa agaggagcga tactggtagg tggcacggtg aatggggaaa
      421 gtgatgctat atgtttgaca ttaccaaaat cgggtattgc tttccgccct accctgtgac
      481 catcgagcgc acgaaattgc gggaactggc tctggctatc ggcgatcccg atgtagtgta
      541 ccaggatcgg caagcggctc atgcggcagg ttacgaggat gtgccgctgc ctcccacaac
      601 ggctaccata ttgctcttct gggaaaacag acatttttta gaacaactcg tcgaattggg
      661 gctggatatc tcgcgtttaa tccaccggga agaaacgtat gagtacctgg ctccacttca
      721 tcctggcgaa acgctgatgg gggtgatgac cgtactcgat ggcgctaccc gcaaaggtcc
      781 tcgcggtagc gccatcgatc ttatcacgct gcaactgcgc tataccaatc gggaggggca
      841 gcttgcgttg attgccacga cgcgactcgt cacgcaatag tggcgcatta cttttccttc
      901 tccagcgaac tcttggtatt gcgctcgccg tcgtaggtca gcaggatgcg tttgccctcg
      961 tagagtgtct ccaggtggat ggggcgaccc cacagggtgt aaacgtgctg gagcgtcttt
     1021 tcggcgtaga ccatgtcgag ttcctgaccc tcgtagaggt gcttgatgta gagttcgcgg
     1081 ttgccccggt aatcgccatt atcgacaacg agataaggaa cgccgaagtt cgtcatgctg
     1141 gtcacgatag aatcgcgcac cttctcccag ttcttctcga cgatgaccca ctcgtcgccc
     1201 tctttgcggt aaaggtagag gtcaaggtct ttcaccaggt cttcggtcag gtagttgcgc
     1261 aggaaggaga catcgttctc catctcgcgt acctcgaaga ttttcgcctt gccctggccc
     1321 ggcttgcggc ccaggcgttc ctgctcttcc ctggtcgggt tgtcccagcg ccgctcgatg
     1381 tcctcgaaga ccttgtagcc gatgtagtag gggttcaggt aggtgcgcga gggtgagagg
     1441 acgccggagt gcagttgggc aaattcgatg gtgtcggaat cggagatagc gcccgtttcg
     1501 cccaggcggc gcatgatgcg cgagtgccag gtgctattgt ggtgtaacat accgttggcg
     1561 acatagcgat gtgtgcggtc caccgtgatg tcgtatacgt cggccacgtc atgctcgatg
     1621 ctgactacct cgtcgaatgg ctcttccagc ctgaaccagc gatggctggc caggtagtct
     1681 tgcaggttgc cctgcttgcg cgccagcccg aaaccaatct cttgcgcgta gagctgcgcc
     1741 gatttgccgc tgatccacac gttgacacct cgtcgccgcg acaggatgcc gtagttcaac
     1801 agcaggattt gcagcgtccg ggcaatctca tcgctgaagg tggagagaat gacgccctgc
     1861 ttgacggagg cgcagccgtc gcagtcgaaa taggcgcgca ggaaggcgct gacgaccgct
     1921 ttgggcgagc gcaagatgca atcaggaatg cgtttatcgc gcgccttcgc gtggaggtcg
     1981 atacccagag attgtaacaa gtcaagtacg ttggcagaat agaagacgac gcgccatctg
     2041 ccgcccttgc cattggcggt gtgggcatcc cagaatggcg ccgcttcgat ggcgaacaat
     2101 tcgcggacga gcgcggcgta gcggtcggcc aattcgcggt cgcccgatgt gtagccgatt
     2161 gcgttcttgg tgacatgaat attgccatcg ccgaccaggt agccgaggaa ttcggcgaac
     2221 gcctctgtta catgcgtggg cgcgaccagc ggaaaacggc ggctatacag cggcttgccg
     2281 ctattgccag gcgtaa
//
LOCUS       DASZAK010000096         1805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_215342, whole genome shotgun sequence.
ACCESSION   DASZAK010000096 DASZAK010000000
VERSION     DASZAK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215342"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..1392)
                     /locus_tag="VGT44_01550"
     CDS             complement(169..1392)
                     /locus_tag="VGT44_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579510.1"
                     /translation="MTDTSASPKTILCLTSYEKGQEFMREAKRQGWKVILLSVTALEH
                     ADWPRDSLDELFFMPDLSKVEDVINGVSYLARTRIINRIVALDDYDVWTGAALREHLR
                     VAGMGETTVRYFRDKLSMRLKAQEYGIPIPDFVHVLNYDRIRAFMESVPPPWMLKPRA
                     EASTIGITKIASPDELWPRLDALGDRQSFYVLERYLPGEVFHVDSLVYDRQVVFAETH
                     QYGRPPLDVFHGGGIPTTRTISRKSAEALTLRELNRKVIQSFGLLQGATHLEFIKGSD
                     DGQFSFLEAAARVGGANIADLIEHSTGINLWREWARIETATEDNPYQLPYYRQDYGGV
                     IITLARQEHPDTSAYTDPEIVYRVVKRHHVGFVVAAKNPERVNTLLEDYSRRFNDDFH
                     TSLPPFESRPPSSSE"
     gene            complement(1515..>1805)
                     /locus_tag="VGT44_01555"
     CDS             complement(1515..>1805)
                     /locus_tag="VGT44_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579511.1"
                     /translation="SLTSTIGPIEYRMVRQFVIKRLSQLDNVSSKRSNAFGRSSQKKL
                     PQDIVDRVDLYIDAYRSLVIVEARRSKELYPYEGILLVRQGKPSIDQAATYY"
BASE COUNT          397 a          481 c          517 g          410 t
ORIGIN      
        1 aacaaaaatg agcataatcg aacaatcgtg ttctgcagta agagcaatac actcgcatag
       61 agacaattag gctgcatatc tctcaatgca gcatctcccc caaagattta tagttgctgt
      121 aattaagagt atctgcttgt cgatgaaatg tcaatcccac cgaccaaatt actcgctact
      181 actaggaggg cggctctcga aggggggcag gctagtatgg aaatcgtcgt tgaagcggcg
      241 actgtagtct tcgagcaggg tattgacacg ctcaggattt ttcgcggcga cgacgaaacc
      301 gacgtgatgg cgtttcacca cgcggtagac gatctccggg tcggtgtagg ccgaggtgtc
      361 ggggtgttcc tggcgcgcca gggtgatgat gacgccgccg tagtcctgcc tgtagtacgg
      421 caactggtag ggattgtcct cggttgctgt ctcgatcctg gcccattcgc gccagaggtt
      481 gatgccggtc gaatgctcga tcaggtcggc gatgttcgcg ccgccgacgc gcgcggcagc
      541 ttcgaggaag gagaactggc catcgtcgct gcctttgatg aattccaggt gtgtcgcgcc
      601 ctgcaataat ccgaacgact ggatgacctt gcggttgagt tcgcgcagag tcagcgcctc
      661 cgccgatttg cgtgagatgg tgcgcgtcgt ggggatgcca ccgccgtgaa agacatccag
      721 cggcgggcga ccgtactggt gcgtctcggc gaagacgacc tggcgatcat acaccagcga
      781 atcgacgtgg aagacctcgc cgggcaggta gcgttccagc acgtagaacg attggcggtc
      841 gcccagcgcg tcgaggcgcg gccacagctc atcgggcgag gcgatcttcg tgataccgat
      901 ggtcgaggct tcggcgcgcg gcttgagcat ccagggcggc ggtacgctct ccatgaaggc
      961 gcgaatgcgg tcatagttca gcacatgcac gaagtcgggg attggaatgc cgtattcctg
     1021 cgccttgagc cgcatcgaca gcttatcacg aaagtagcgc accgtcgtct cgcccatgcc
     1081 cgccacgcgc aggtgttcgc gcagggccgc gcccgtccac acgtcatagt cgtccagcgc
     1141 cacgatgcga ttgatgatgc gcgtgcgggc caggtagctg acgccattga tgacatcctc
     1201 caccttcgac aggtcgggca tgaaaaatag ctcgtcaagg ctgtcgcgcg gccaatcagc
     1261 gtgttccaac gccgtcacgg acagaaggat caccttccac ccctgccgct tcgcttcacg
     1321 catgaactcc tgcccctttt cgtagctggt aaggcataag atggtcttgg gtgaggcgct
     1381 ggtatcggtc atggtatctt ctcctggaaa gctggctaaa gacgaacttg cgcttacttc
     1441 tataatacca tgtaaatata ctggaaacgt agaagcgaac ctggaagcgc ctgcatggtt
     1501 tacagcattc aacatcaata ataggtggca gcctgatcta tcgatggttt gccctgcctc
     1561 accaggagaa tgccttcata gggatatagt tctttcgacc ttcttgcttc cacaattact
     1621 aatgatctat atgcatcaat atacagatct actcgatcta ctatatcctg tggtaacttt
     1681 ttttgagaag aacggccgaa ggcattggaa cgctttgatg atacgttatc tagctgtgaa
     1741 aggcgtttga tgacaaactg gcgcaccatt ctatattcga tagggccaat cgttgatgtc
     1801 aggct
//
LOCUS       DASZAK010000097         6282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_676947, whole genome shotgun sequence.
ACCESSION   DASZAK010000097 DASZAK010000000
VERSION     DASZAK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6282
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676947"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VGT44_01560"
     CDS             complement(<1..276)
                     /locus_tag="VGT44_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579512.1"
                     /translation="MINLRLRGFLLVGTTACMFGLGVVLAKLITGVLNSFLISFLALA
                     GGGALVTVYLWARRQPVVPPLSRSAWGQMLLLAFVGTALPLILVVEGF"
     gene            complement(291..1208)
                     /locus_tag="VGT44_01565"
     CDS             complement(291..1208)
                     /locus_tag="VGT44_01565"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006157.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HEV2579513.1"
                     /translation="MESDQRVKVAIIGSGNIGSDLMYKLLKQPGHMQLVMLAGIDPAS
                     EGLARARSLGIDASADGLQAILANPEIKIVFDATSARSHVRHAKALREAGRMAVDLTP
                     AARGPYVVPPVNGSDLLDQVNVNMVTCGGQATVPMVYAVSRVVPLLYAEMVSTISSRS
                     AGPGTRQNIDEFTETTSKALEVIGKANKGKAIIILNPADPPILMRNTIYALPGEEFDE
                     KAVLESVARMEAEVQEYVPGYHLKNAPLFDVRDTPWGKKPVVTIFLEVEGAGDYLPTF
                     SGNLDIMTAAARRVGEVVAQRLLTRQGVA"
     gene            complement(1320..2102)
                     /locus_tag="VGT44_01570"
     CDS             complement(1320..2102)
                     /locus_tag="VGT44_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2579514.1"
                     /translation="MEQFVEKVAAELHRAEHQQSPIAPLSETYPSITPEQSYAIQRAW
                     LEIKRREKGDAFRLFGHKIGLTSLAMQKQLGVDQPDYGFLLDTMVLPSGGTAAFQDFI
                     MPRIEPEIGFWLAQDVQGPGVTAAMVLAATRGVSAALELVDSRIEKWRIKLVDTIADN
                     ASSARVIVSEREISPNDLAAEEVTLLRNGEPVGAGNGAAVLGHPAEAVAWLANKLAEF
                     GIGLKAGQFVLPGAMCASALVSPGEEYRARFAHLGDVSISFV"
     gene            complement(2138..3142)
                     /locus_tag="VGT44_01575"
     CDS             complement(2138..3142)
                     /locus_tag="VGT44_01575"
                     /EC_number="1.13.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018577 - catechol 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 2,3-dioxygenase"
                     /protein_id="HEV2579515.1"
                     /translation="MSEVQEPIKDVAHLGHFEIFTPKPDESLWFFTDAFGMTETNREG
                     QSVYLRAWGDYDHCTLKITEAKQAGLGHVGWRAVSPQALDRRVKALTEQGYGKGWIEG
                     DIGHGRAFRFTDPDGHLMELYYESERYRAPDEQRSNLFNQQQRHVGHGVNVRRIDHVN
                     LMCSDITPNRKFLQELLGFQLHEVLQPVEDGVEEGAWLSVTNLTHDIAYTRDFTGNHG
                     RLHHIAYWLDTREDVLRAADILTEHGVFIEAGPAKHNISQAFFLYLYEPGGNRVEIYS
                     GGYLAFAPDWEPFIWRKAHRGRGVYWGGTLPESFKTYGTPVIDVPTPNKGEVFVFDPA
                     "
     gene            complement(3161..3937)
                     /locus_tag="VGT44_01580"
     CDS             complement(3161..3937)
                     /locus_tag="VGT44_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV2579516.1"
                     /translation="MLQTVQKAAEVLNLFSVDHPEWGISELSKTLAFPKSSTSALVTT
                     LTEQGLLRRTNAGRYKLGWRVMALSQVLLSTTDFRTEARRVMESLIARFGETVHLAAL
                     ESGQVIYVDKLQGTRAIQVSVTGVGYRLPAHCSGVGKTILADRPWEEVLLILNARGMP
                     PLTPHTITSPEAFRQELERVSAQGYAYDLEEAVEELCCVAAPIRDHSGEVVAAMSLSV
                     PAYRFQQYKDQYRLAIVGAARQVSENLGYIAGKCCRKKSV"
     gene            complement(4082..4948)
                     /locus_tag="VGT44_01585"
     CDS             complement(4082..4948)
                     /locus_tag="VGT44_01585"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2579517.1"
                     /translation="MDVKNLVAIDVHTHAEVSSRVPDDPLWKAMQEASADYFKDEGPR
                     PTIDEVAAYYRARRIAAVIFPVDMESTTGIPRVSNEEVAETAAQHADVLIPFASIDPA
                     KGKMGAREARRLIEHFGVRGFKFHPPMQAFYPNDRSAYGLYEVIQEAGLPALFHTGHS
                     GIGSGMRGGGGVRLKYSNPMYLDDVAVDFPDMPIIMAHPSFPWQDEALSVCLHKPQVY
                     IDLSGWSPKYFPPQLIQYANTLLKHKMLFGSDYPLITPDRWMADFEQIAIRPEARPLI
                     LKENAVKLLKLG"
     gene            complement(4976..>6282)
                     /locus_tag="VGT44_01590"
     CDS             complement(4976..>6282)
                     /locus_tag="VGT44_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2579518.1"
                     /translation="NPSYKEREVAYQMHNCEAAAVIVQYELLPLVQAVRSEVPSLRWV
                     IVVGAGERSLPVDVISYSRILQTQSSMAPTGNEPGGEDVVALPYSSGTTGMPKGVMLS
                     HKNLVCNAYQSVATARITSQDRMLVFLPLYHIYGIMLMGLAATTGAKIVLMERFEPEQ
                     CLRHIDEQGITLLYTVPQILTALLNWPTLDNYNLHSVRFAQCGAAPVPPVLAHRFQER
                     TGITVMTSYGLTEASPGTHSNPVYDRKLIKVETIGLPVHDTRQKIVDIESGSIELGVG
                     EEGELIVQGPQVMLGYWKAEEATAQALRDGWLYTGDIGFRDADGYVTITDRKKEMIKY
                     KGFSVAPAQIEAMLLEHPAVLDVAVIAKADEEAGEVPKAFVVLRTGHENQSASELMMW
                     ANGKLATFKNVREIEYIDAIPRNPSGKILRRVLKEREQQRTT"
BASE COUNT         1288 a         1932 c         1723 g         1339 t
ORIGIN      
        1 gaaaccttca acaactagaa tcagcggtaa agccgtcccg acgaatgcca acaagagcat
       61 ctgcccccac gcggagcgag agagaggtgg cacaactggc tgccgtctgg cccacaaata
      121 cacggtgacg agtgcgccac cacccgcgag agcaaggaaa gagataagaa acgagtttaa
      181 gacgccggta atcagcttcg ccagcacaac tcccaggcca aacatacagg cggttgttcc
      241 gaccagtaga aagccccgta gtcgcagatt aatcacatgc tctttcccga tcaggccact
      301 ccttgtcgcg tcagcaggcg ttgcgctaca acttccccga cgcggcgcgc ggcggcggtc
      361 atgatatcga gattacccga aaatgtcggt aggtaatcac cggcgccctc gacctccagg
      421 aaaatcgtca ccaccggctt tttgccccag ggagtatcgc gcacgtcgaa cagtggcgcg
      481 ttcttcaagt gatagcccgg cacgtattcc tgcacttccg cctccatgcg cgcaaccgat
      541 tcaagcacag ctttctcgtc aaactcctcg cctggcaggg cgtagatggt gttgcgcatc
      601 aaaataggag gatcggcagg attgaggatg ataatggctt tgcccttgtt cgccttgccg
      661 atgacttcca acgctttcga ggtggtttcg gtgaattcgt cgatgttctg gcgcgtacct
      721 ggcccggcgg aacggctgga gatcgtgctg accatctccg cgtacaggag cggtacaacc
      781 cggctgacgg cgtagaccat cggcacagta gcctgtccgc cgcatgtcac catattgacg
      841 ttgacctggt caagcagatc gctcccgttg accggcggca ctacgtaagg tccgcgcgcc
      901 gccggcgtca ggtctaccgc catgcgtccg gcctcgcgca gcgccttcgc gtggcgcacg
      961 tgtgacctgg cactggtggc gtcaaacacg atcttgatct caggattggc cagaatcgcc
     1021 tgcaagccat cggcgctggc gtcgatgccc agcgaccgcg cgcgcgccag cccctcggat
     1081 gctggatcga tgccggccag catcaccagt tgcatgtgtc cgggctgctt gagcaacttg
     1141 tacatcaggt cactgccaat attgccggaa ccgataattg ccactttgac gcgctgatct
     1201 gattccattg ctatcctcct ggactaattc cagtttgcat cacaattcgg cccttcacca
     1261 gggacgcggg cgacgcaagc gtccccaccc cacatccgca ccacccccgc ccctacggat
     1321 tatacaaacg atattgagac atcgccaaga tgggcgaatc ttgcgcggta ctcttcgcct
     1381 ggcgagacaa gcgcggaggc gcacattgcg ccgggaagca caaactgacc ggctttcagg
     1441 ccaatcccga actcggccag cttattcgcc agccatgcca ccgcctccgc cgggtggccc
     1501 agcacagccg cgccgttgcc cgcgccgacc ggctcgccgt ttcgcagcag cgtcacctct
     1561 tcggcagcga ggtcattcgg ggaaatttct cgctcactca caatcacgcg cgccgaggat
     1621 gcattatcgg cgatggtatc gactagcttg atgcgccatt tttcgatgcg gctatccacc
     1681 agctccagcg cggcgctgac gccgcgtgtc gccgccaaca ccatcgccgc cgtcacgcca
     1741 ggcccctgca catcctgggc cagccagaag ccgatttccg gttcgatgcg cggcatgata
     1801 aaatcctgaa aggctgctgt accgcccgat ggaagaacca tcgtatcaag cagaaagcca
     1861 taatcgggct gatccacccc cagctgtttc tgcatggcca ggctggtcag gccgatctta
     1921 tgaccaaaga gccggaaagc gtctcccttc tcccggcgct tgatctctag ccacgcgcgt
     1981 tgaattgcgt aggattgctc aggtgtgatg gatgggtagg tttcgctgag cggcgcgatg
     2041 ggcgattgct gatgttccgc gcggtgcagc tcggcggcaa ctttctctac aaactgctcc
     2101 atggactccc ctttttcctc tccgcatatg ttcgttgcta tgccgggtca aagacgaaga
     2161 cttcgccttt gttcggcgtg ggtacgtcaa taacgggcgt accataggtc ttgaaacttt
     2221 ccggtagcgt acctccccaa tatactcctc tgccgcgatg ggccttgcgc cagataaacg
     2281 gctcccaatc gggcgcgaag gccaggtagc cgccagaata aatctcgacg cggttcccgc
     2341 ccggctcgta gagatagagg aaaaaggctt gcgagatatt atgtttggcc gggccggctt
     2401 cgatgaaaac gccatgctcg gtcaagatat ccgcggcgcg cagcacatcc tcgcgcgtat
     2461 cgagccagta agcgatatga tgcagcctgc cgtgattgcc ggtgaagtcg cgcgtatagg
     2521 cgatatcgtg tgtcagattg gtgacgctga gccacgcgcc ctcttcaacg ccatcctcca
     2581 ccggctgcaa aacctcgtgc agttgaaagc cgagtagttc ctggagaaat ttacgattcg
     2641 gcgttatatc gctgcacatc aggttgacat gatcgatgcg ccggacgttg acgccgtgtc
     2701 cgacatggcg ctgctgctgg ttaaacaggt ttgaacgttg ttcgtcgggc gcgcgatatc
     2761 gttccgactc gtagtacagt tccatcaaat gcccgtcggg gtcggtgaat cgaaaggcgc
     2821 ggccatgccc gatatcgccc tcgatccagc ctttgccata gccttgctcg gtgagcgcct
     2881 tgacgcgccg atcgagcgcc tgggggctga ctgcccgcca gccgacgtgc cccaggccgg
     2941 cctgtttcgc ctcggtgatc ttgagcgtgc agtggtcgta gtcgccccac gcgcgcaaat
     3001 agaccgactg gccttcgcgg ttcgtttccg tcataccgaa ggcgtctgtg aagaaccaga
     3061 gactctcatc gggtttcggc gtgaatatct caaaatgacc caggtgggca acgtctttga
     3121 tcggttcctg tacttccgac atggcaccag ctcctttgcg ctacacgctc ttctttcgac
     3181 aacacttgcc cgcgatatac ccaagatttt ccgatacctg ccgcgcagcc cccacaatcg
     3241 cgagtcgata ttgatccttg tactgctgga agcgataggc cggcaccgac aggctcatgg
     3301 ccgccacgac ctcgccgctg tgatcgcgaa taggcgcggc gacgcagcac aattcctcga
     3361 ccgcctcctc aaggtcatag gcatagccct gcgcgctgac tcgctcaagt tcttgcctga
     3421 aggcttccgg cgaggtgatc gtatgaggtg tgagcggcgg cattcccctg gcattcaaaa
     3481 taagcagcac ttcttcccag gggcgatccg ccaggattgt tttgccgacc ccgctgcaat
     3541 gcgccggtag acgatagcca actcccgtga cagagacctg gattgctcgc gtcccttgca
     3601 acttatccac gtaaattacc tgcccactct ccaacgccgc caggtgtacg gtctccccaa
     3661 aacgcgcgat cagcgactcc atgacgcggc gcgcctccgt gcggaaatcg gtcgtggaaa
     3721 gcagcacctg gctgagcgcc atgacacgcc agcccagctt atatctgcca gcgttggtgc
     3781 ggcgcagcag accctgttcg gtcaaagtgg tgacgagcgc ggacgtgctg gatttgggga
     3841 aagccagggt cttcgacagc tcgctaatgc cccattcagg atgatcgacg gagaagagat
     3901 tgagtacctc ggctgctttt tgaacagtct gtaacatacg tgcgcgcctt tctacaatga
     3961 agaggctcga tccagccgac tatattaccc aaagtataat cggttccgtt cgattcaaca
     4021 acatagccgt tctactatcc tgaacacatc cattgtatcg tagatttttg tcgcgtccct
     4081 actaaccaag cttcaacagc ttcacggcat tctccttcag gatcaacggc ctcgcctctg
     4141 gtctgatggc gatttgctcg aaatctgcca tccagcggtc aggcgttatc aagggataat
     4201 cgctgccaaa gagcatcttg tgcttgagca gggtattcgc gtactggatc agctgaggcg
     4261 gaaagtattt cggcgaccag ccggaaaggt cgatataaac ctgcggcttg tgcaggcaga
     4321 ccgagagcgc ctcgtcctgc cagggaaacg acgggtgagc catgatgatc ggcatatcag
     4381 gaaaatctac cgcgacatcg tcgagataca tcgggttgga gtattttagt cgtacaccac
     4441 caccgccgcg catcccgctg ccaatccccg aatgcccggt atgaaagagg gcgggcagac
     4501 ctgcctcctg gataacctcg tacagaccgt aggcgctgcg gtcgttcggg taaaacgcct
     4561 gcatgggcgg gtgaaacttg aagccgcgca caccgaaatg ctcgatcagc cgccgcgcct
     4621 cgcgcgcgcc catcttgccc ttcgccgggt cgatgctggc gaaggggatc agcacatcgg
     4681 catgttgagc ggcggtttcg gccacctcct cgttgctgac acgcgggatg ccggtggtgg
     4741 attccatatc gaccggaaat ataacagcgg cgattcgtct tgcgcgataa taagcagcga
     4801 cctcgtcgat agtgggacgc ggtccctcgt ctttgaaata gtcggcagat gcctcctgca
     4861 tggccttcca gagcgggtca tcaggcacac gactagatac ctccgcgtgg gtatgcacgt
     4921 cgattgcaac caggttcttt acatccatat tactttgctt tctgtcatgg tttatctacg
     4981 tcgttcgctg ctgttcccgc tccttgagaa cgcggcgcag gattttaccg gacgggttcc
     5041 gcggaatagc atcgatatat tcgatctcac gcacattctt aaaggtggcg agcttgccgt
     5101 tagcccacat catcaattcg ctcgcggact ggttctcgtg gcccgtcctc agtacgacaa
     5161 aagctttcgg tacttcgcct gcctcctcgt cagctttcgc aattactgcc acatctagca
     5221 cggcgggatg ttcgagcagc attgcctcga tctgggcggg agcaacgctg aatcctttgt
     5281 atttaatcat ctcctttttg cggtcggtga tcgtgacgta tccgtccgcg tcgcgaaagc
     5341 cgatgtcgcc ggtgtaaagc cagccatcgc gcaaggcctg ggccgtcgct tcttcagcct
     5401 tccagtagcc cagcatcacc tgcggcccct gcacgatcaa ctcgccctct tcgccaacgc
     5461 ccagttcaat actgccgctc tcgatatcaa caatcttctg gcgcgtatcg tgaaccggca
     5521 ggccgatcgt ttcaaccttg ataagcttcc gatcataaac gggattggag tgcgttcccg
     5581 gtgaagcttc ggtcaggcca taggaagtca tcacggtgat gccggtgcgc tcctggaagc
     5641 gatgcgccag cactggcgga acgggcgccg cgccgcactg ggcaaagcgc acgctatgca
     5701 ggttgtagtt gtcgagcgta ggccagttca gaagcgccgt caatatttgc ggcacagtat
     5761 aaagcagcgt gatgccctgc tcgtcaatat gccgcaggca ctgctccggc tcgaagcgtt
     5821 ccatcagcac aatcttcgcg cccgtcgtag cagccaggcc catcagcatg ataccgtaga
     5881 tgtgatagag cggcaggaag accagcatcc ggtcttgcga cgtgatgcgc gctgtcgcta
     5941 ccgattgata ggcattgcaa accaggttct tgtggctcag catgacgccc ttgggcatcc
     6001 cggtcgtgcc gctactgtaa ggcagtgcaa ccacatcctc cccccccggc tcattgcctg
     6061 ttggagccat cgaggattgc gtctgtagga tgcgactata tgaaatgaca tcgacaggca
     6121 gcgatcgttc gcccgcgcca acaacgatca cccaccggag cgagggtact tccgaccgaa
     6181 ccgcctgcac cagtggcagc aattcatact gtacaatgac cgccgccgct tcgcagttat
     6241 gcatctgata tgctacttcg cgctccttat atgatggatt ca
//
LOCUS       DASZAK010000098         3776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_428565, whole genome shotgun sequence.
ACCESSION   DASZAK010000098 DASZAK010000000
VERSION     DASZAK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428565"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VGT44_01595"
     CDS             <1..685
                     /locus_tag="VGT44_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579519.1"
                     /translation="CLVIAVSTQQAFYKLVQETPLLQQLFFDTPTNTNAAMLGTFLFT
                     FMPPLVVAFALALALKWAADLDNGRLEVLFSTPQSRRKVLLSRFCANVLLILLAPLLT
                     WLAVLMGAQLSHIAVDQGRIFAASFNMFPMALITIGLVYALAGRLRYATLVSLLSAYL
                     VLAYIQELLEGTIPMPTWLMNLSIFHVYGNPVFLGVNWTNFLGMTGVALVLLLISLLQ
                     FRTADITSG"
     gene            944..1933
                     /locus_tag="VGT44_01600"
     CDS             944..1933
                     /locus_tag="VGT44_01600"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV2579520.1"
                     /translation="MLPVKLAGLGWYLPPRRVTNAELEAQLDIPADWIERVTGVCERR
                     YVSDETVVAMGAAASCMALDAAHMQVEDLDAIICASTSPQQAIPCTAAFLQRELQAPE
                     GMSACFDINATCLSFLFALQTAAHLVAAGAYSSMLICCSEIVSRFLNPGERESVALFG
                     DAAAACVVTRRAPGEASAFWHAHFETYNSGADLTEIRGGGTLHHPNDPQTTPEMNLFH
                     MNGPSVFRQASMLGEPVMDHFMNELGWERGCFDALVPHQASRHAIEQFTRRLGFSKAQ
                     MIVNLPQRGNCVSASIPLALAEAVHSGRIRRGDRVLLAGTGAGLTLGALAITY"
     gene            2005..2991
                     /locus_tag="VGT44_01605"
     CDS             2005..2991
                     /locus_tag="VGT44_01605"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV2579521.1"
                     /translation="MDILVTGGTGFLGQHLARGLLAKGHNVRLLGRNFATVGTKRLVA
                     DGAEPIEADLRNREAVQAACADVEAVCHVGALSSPWGRRVDFYDINVGGTEAVLAGCL
                     AHGVRRLVYISSPSVVFDGRDQRLVTEDAPYPRRFPSVYSWTKKLGEDRVRLAAAQGL
                     QTVILRPKAIFGPGDQALLPRLIDAARQKRLPQIGDGRNLVDLTYVENVVHAIMLALD
                     SQSAIGNIYTITNDEHVPLWDVIRLVLERLKLSTDLRRIPLPVALAAAALMETRASLT
                     GKEPLLTRYSAAILARTQTYDISAAKRDLGYTPRIPIADGIQCTLAALEEHR"
     gene            2988..>3776
                     /locus_tag="VGT44_01610"
     CDS             2988..>3776
                     /locus_tag="VGT44_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2579522.1"
                     /translation="MSKDSSAPMLECHLLDTGYCLAWEHHVMRGGRRVRIACHSLVAL
                     LRHPEHGWLLWDTGYAPRMLEATQRLPFRLYRYATPLRLNPDLAVIRQLAKRGIAPRD
                     IRRVIISHFHADHIAGLRDFPDAEVIASRDAYEEVVARSGISALSRAFIPALLPGDFS
                     QRARLIETFEGPSLPALGAAHDLFGDGSLLLIPLPGHARGQVGLLARTVRGHVLFAAD
                     GSWLRQAIREDRPPARVTHLFVDDARAARATIARLHNFAAACPDV"
BASE COUNT          708 a         1144 c         1128 g          796 t
ORIGIN      
        1 ctgcctggtg atcgctgtga gcacccagca ggcgttctac aaactcgtac aagagacccc
       61 tttgctgcaa caactctttt tcgacacgcc caccaacacc aacgccgcga tgctgggtac
      121 attcctcttc acgttcatgc ccccactcgt cgtggccttc gcgctggccc tggcattgaa
      181 gtgggccgca gacctggata acgggcgact ggaagtactc ttcagcaccc cgcaatcgcg
      241 aagaaaggtc ctgctctcgc gcttttgcgc caatgtcctg ctcatcctgc tggctccgct
      301 gctgacctgg ctcgcggtcc tgatgggagc gcaactctcc catattgccg tcgaccaggg
      361 caggatcttc gcggccagct tcaacatgtt cccaatggcg ctgattacca tcggcctggt
      421 ctacgcgctg gccggacgct tgcgctatgc cacgctagta agcttgctct ctgcatacct
      481 tgtgctggcc tacatacagg aattgttgga gggaaccatt cctatgccta cctggctcat
      541 gaatctctcg atcttccacg tgtatggcaa cccggtgttc ctcggcgtca actggacgaa
      601 ctttctgggc atgaccggcg tggccctggt gctgttgctt atcagcctgc tccagttccg
      661 cactgccgat atcacgtcag gctgaaccta tcttgtgaga agggagaaat atgagacgat
      721 caagtctgtc atccagagcg cagcgaagaa tcccacgcac cccaacgacg aggccgtagt
      781 tcttaaatag tgcattcgtc ccatgcgtaa aacgccaaaa cagtgtatca ttatcgtaca
      841 ggttataggc cagctccgtg ccagagtgaa acttgtcagg gtaagtggct acgagtaaca
      901 ttagacgact gatggtatat gttacggaaa ggtgggatcg cgaatgcttc cggtgaaact
      961 tgcgggttta gggtggtatt tacctccacg acgagtcaca aatgcagaac ttgaagcgca
     1021 gctagacatt ccggcggact ggatcgagcg cgttacggga gtgtgcgagc gccgctacgt
     1081 cagcgatgaa acggtggtgg cgatgggtgc tgcggcttcg tgtatggcac tagacgcggc
     1141 gcatatgcag gtagaagacc tcgacgcgat catctgtgcc tcgacatcgc cgcagcaggc
     1201 gataccctgc acggcagcct tcttgcagcg cgagctacaa gcgccggagg gcatgagcgc
     1261 ctgttttgat atcaacgcca cctgtttgag cttcttgttc gccttgcaga ccgccgctca
     1321 tctcgtcgct gccggagcgt attcatccat gctgatctgt tgcagcgaga tagtttcgcg
     1381 attcctcaat cctggcgaac gcgaaagcgt ggcgttgttc ggtgacgcgg ctgccgcctg
     1441 tgtcgtcacg cgccgcgcgc cgggcgaggc cagcgcgttc tggcatgccc acttcgagac
     1501 ctacaacagc ggggcggacc tgaccgagat tcgcggcggc ggaacgctgc atcatcccaa
     1561 cgatccgcag actacgccgg agatgaacct gttccacatg aacggcccat ccgtgttcag
     1621 gcaggcatcc atgctgggtg aaccggtcat ggatcatttc atgaacgagc ttggatggga
     1681 gcgcggctgc tttgacgcgc tcgttccaca ccaggccagc cgccacgcca tcgagcaatt
     1741 cacgcgccgc ctgggtttca gcaaggcgca gatgatcgtc aacctgccgc agcgcggcaa
     1801 ctgcgtctcc gcctccattc cgcttgcgct agccgaagca gttcacagcg gacgcatccg
     1861 gcgcggcgac cgcgtgctgc tggccggtac gggcgcgggc ctgacactgg gcgcgctggc
     1921 tataacgtat tgataagtgc aatgaaattc ttgtaaggca cctacagtgt ccctcgtgtt
     1981 cgttttccac aaataaggaa agacatggat atcctagtca ccggcgggac aggctttctc
     2041 ggccagcatt tagcgcgtgg gctgctggcg aagggtcata atgtccgctt gttgggtcgc
     2101 aatttcgcta ctgtgggaac gaaacgactg gttgctgatg gagctgagcc gattgaggcc
     2161 gacctgcgca acagggaggc ggtacaggcg gcttgcgcgg atgtcgaggc ggtctgtcac
     2221 gttggcgcgc tctcgtctcc gtggggcagg cgcgtcgatt tctatgacat caatgttggt
     2281 ggaacggagg cagtgctggc cggctgcctg gcgcatggcg tgaggcggct ggtctacatt
     2341 tcgtcgccga gcgtcgtctt cgatggacgc gatcaacgtc tcgttaccga agatgcgccc
     2401 tacccccgcc gcttcccctc ggtctattca tggacaaaga agctgggcga agaccgcgtt
     2461 cgcctggcgg cagcgcaggg cttgcagacg gtgatcctgc ggccaaaggc gatcttcggg
     2521 ccgggcgacc aggcgctgct gccccgcctg atcgatgccg cgcgtcagaa acgcctgccg
     2581 caaattggcg atggacgcaa cctcgtggat ttgacctatg tagagaacgt agttcatgcg
     2641 atcatgcttg cgctcgacag tcaatcggct ataggaaata tttatacgat cacgaatgat
     2701 gagcatgttc cattgtggga tgtcatcagg ctggtgctgg aacgactcaa gctctcgacc
     2761 gatctgcgac ggataccgct gcctgtcgcg ctggctgcgg cggcgctgat ggagacgcgc
     2821 gcgtcgctga ccggcaaaga accgctgctg acacgctact cagccgcaat cctggcgcgc
     2881 acacagacct atgatatcag cgcggcaaaa cgcgatcttg gctatacacc gcgcatccct
     2941 atcgccgatg gcattcaatg cacgcttgcg gctttggagg agcatcgatg agcaaggata
     3001 gtagcgcccc aatgctggaa tgccacttgc ttgatacggg ctattgcctg gcgtgggagc
     3061 atcatgtcat gcgcggcggg cggcgtgtgc gcatcgcctg tcattcgctg gtggcactgc
     3121 tgcgccatcc agaacacggc tggctgctgt gggacaccgg ctacgcgccg cgtatgctgg
     3181 aagccacgca gcgcctgcct ttccggctct accgctatgc cacaccgctg cgcctgaatc
     3241 ctgacctcgc ggtcatccgg caactggcga aacgcggtat tgcgccgcgt gacattcgcc
     3301 gggtcatcat ctcacacttc cacgcggacc atatcgcggg tctgcgcgat ttccccgatg
     3361 ctgaggtgat cgcgtcccgc gacgcttacg aagaggtcgt cgctcgtagt ggcatcagcg
     3421 cgctcagccg cgcctttatc cctgctttat tgcccggtga tttcagccag cgcgccaggc
     3481 taatagagac atttgagggg ccgtcgctgc cagccctggg agctgctcac gatctgtttg
     3541 gagatggttc gctgctgctc ataccgctac ctggccatgc tcgcggccag gtgggactgc
     3601 tggcgcgcac cgtgcgcggg catgtactct ttgccgccga tggaagctgg ctgaggcagg
     3661 cgatccgcga ggaccgcccg cccgcccgcg tcacgcatct gttcgtggat gacgcgcgag
     3721 ctgcacgcgc caccatcgcg cgcctgcaca acttcgccgc cgcctgtccc gacgtg
//
LOCUS       DASZAK010000099         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_250923, whole genome shotgun sequence.
ACCESSION   DASZAK010000099 DASZAK010000000
VERSION     DASZAK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250923"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VGT44_01615"
     CDS             complement(<1..268)
                     /locus_tag="VGT44_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2579523.1"
                     /translation="MFKRILVPLDGSTRAERALTIAARLARASGGSITLLQAVGIPAD
                     YRMYAYGAYPAQAPVFAQEALTTEKAQAEAYLATILQWEVLAGIP"
     gene            589..1041
                     /locus_tag="VGT44_01620"
     CDS             589..1041
                     /locus_tag="VGT44_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV2579524.1"
                     /translation="MAEMMTAVAKLEAGMRFEGEAGSGHYVSLDASENSGGNNAGFRP
                     MELLLVGLAGCTGMDVISILRKKRQEVTGYEVHVQAVRAETHPMVFEEITVEHIITGH
                     HIDHEAVRRAIELSEQRYCGAGAMLGKVAALRHMYCIVEAEIPALTEA"
     gene            complement(1193..>1623)
                     /locus_tag="VGT44_01625"
     CDS             complement(1193..>1623)
                     /locus_tag="VGT44_01625"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2579525.1"
                     /translation="IIIASTASVYAIIAPLHGLVFPSSALGWIAIFAIALVSTVLAFV
                     TFFAGLKRIGPTTASTLSTFEPIVAVALAAVVLGETITPVQALGGALILVAVVILSRN
                     EMRRTKQSPSQENEDNIETLTPPLAEETKTELFVGGQHET"
BASE COUNT          365 a          442 c          474 g          342 t
ORIGIN      
        1 tggggatgcc tgccagcacc tcccattgca gaatggtggc gagatacgcc tctgcttgcg
       61 ccttttcggt agtcagcgcc tcctgcgcga acactggagc ctgtgccggg tatgcgccat
      121 acgcatacat gcgatagtcg gctggaatac ctactgcctg tagcagtgta atgctgccac
      181 cgctcgcacg cgcaagacga gcagcaatcg tcagggcgcg ctctgctcgc gttgatccat
      241 cgagggggac aagtattcgc ttgaacatat tcgttctcct tcttaatcgg gatcgctcct
      301 gctaacacca ggccccaatc cgctatcctg catccatgga cttccgcctt ctttcgggtc
      361 ttgtctcaag catactatcc tcacatcaca aaacactcac atgtatggtg tctggcgtca
      421 aaagtacgtc acagcgacga ggctttgcct ttcctgggcg atgtaaaaag taccctgtct
      481 atttgtgagc cgaacgtgat gtgccagcta caccttgtga tgcccaggag agggagacag
      541 gctatgatag tatcagagaa tggtttcagt cttgaaagga gtaatccaat ggcagagatg
      601 atgacagccg tcgcaaaact tgaagcaggg atgcgctttg agggcgaagc gggttctgga
      661 cattacgtct cactcgatgc atccgaaaac agcggaggaa acaacgcggg tttccgcccg
      721 atggaactat tactggtcgg actggccggc tgcaccggga tggatgtaat ctccatcctg
      781 cgcaagaaac gccaggaggt gaccggctac gaggtccacg tgcaggccgt gcgcgccgaa
      841 acccacccga tggtcttcga ggaaatcacc gtagaacata tcatcacggg tcatcacatc
      901 gatcacgaag cggtcaggcg ggcaatcgaa ctctccgagc agcgctactg cggcgccggt
      961 gccatgcttg gcaaggtagc cgcgctcagg catatgtact gcattgtgga agcggagata
     1021 ccggcgctga ccgaggcata gttcgcccat taccaaccga gttgagggca gggggaaaga
     1081 ggaggtgaac ccatatgacc agagactgtc tctactgtgg tcttcagttc tctgataccg
     1141 tcaggttttg ccccagctgt ggcaggctca cagaaaaagg cttcaaaatt cgttacgttt
     1201 catgttgtcc tccgacgaat agttcagttt tcgtttcttc cgctagagga ggtgtaagag
     1261 tctcgatgtt gtcctcgttc tcctgggatg gtgattgctt agtgcggcgc atctcgttcc
     1321 tggacaggat cacaacggcg accaggatca gcgcgccgcc gagcgcctgc accggcgtga
     1381 tggtctcgcc cagcacaacg gcggccagcg ccaccgcgac aattggctca aaggtggaga
     1441 gcgtggaggc ggtcgtcggg ccaatgcgct tcagcccggc gaagaaggtc acaaaggcga
     1501 gtacggtgga gaccagggcg atggcgaata tcgctatcca tcccagcgcg ctggaaggaa
     1561 acaccagccc gtgaagaggg gcgatgatgg cgtacacact tgccgtcgaa gcgattatga
     1621 tgg
//
LOCUS       DASZAK010000100         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_73545, whole genome shotgun sequence.
ACCESSION   DASZAK010000100 DASZAK010000000
VERSION     DASZAK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73545"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            560..2071
                     /locus_tag="VGT44_01630"
                     /pseudo
     CDS             560..2071
                     /locus_tag="VGT44_01630"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            2398..2889
                     /locus_tag="VGT44_01635"
     CDS             2398..2889
                     /locus_tag="VGT44_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEV2579526.1"
                     /translation="MDQRRACRAVPNPLIANNPSTRLHSQLDIGATSQASKVQGREPI
                     RLHPTDAATRGIAEGDVVRVFNDRGSCLAGAVLSKDVRPRVAQLSTGAWYDPLDPADV
                     DALCVHGNPNVLTMDQGTSSLAQGCTGQHVLVEIERWNAPVPEITVSRPPPLEQRDGL
                     EVS"
BASE COUNT          582 a          891 c          898 g          585 t
ORIGIN      
        1 acaagaacac atgaaggtcc ataacgatcc tcgctgtttg aaatgggtgg cagggatagt
       61 ttccttatca taaggagagt cggaatggct tgtcaagcca gagggagaaa agtcagaatt
      121 atccaccacc aagtggggca gtacccgcct tcgtttgtac gccagttgtt gcttcctctg
      181 gtgttatctg gtcggatcga gggctttctt gggagggacg agtgaccacc tcaggctagg
      241 gctccagcac gaactctcca tagcagcagg gctgccactt tcaccagcga ggctgccagg
      301 aacagccgcc tacaggcaat tgcggaccat tctctttctg tgatagactc acttggagca
      361 gatgtggcgt cttctggagg ggaaatttga ccgtgtggca catcccgcgt tggggacact
      421 atccctagac ctgccaagaa ctgtggcagg ctccaagcat cgggatctgt ccatcctggg
      481 tattctcaca gaagcggcct gtcgccagga ggggtgctag agtgcctcct ggctgactca
      541 gattgttagg aggtaatcca tgagtgctcg cagcgtccat ccccatagct cgcattgggg
      601 cgtctttgag gccgaagtcg aagatggtca ggtggtgtct gttcatccct acccctacga
      661 cgcccatcct tcccccatct tagccaacat tccagacagc gtccggcatc ctacgcgcat
      721 tagccagccg atggtgcggg ccgggtggct cgatgatggg gcagggccaa gcacccagcg
      781 aggtaaggaa ccttttgttc cggtaagctg ggaaactgcg acggacttgc ttgccgccga
      841 actgcgccgc gtcatcgagc ggcatgggcc gcaggcgatc tacggtggct cctatgggtg
      901 ggccagcgcc gggcgctttc accacgcgca gagtcaggtt catcgcttcc tcaatggtct
      961 gggcggctac acctactcgg tgcacagcta cagcatgggc gccgccgagg tcatctttcc
     1021 acacgtcttc ggcaattact gggatatgct ggtgcgagca accgcctggc ccacgattgt
     1081 agaacacgca gacctgttcg tctgcttcgg agggctgccg ctcaagaata ccgctgtcaa
     1141 acccggaggc attggccgcc accgggtgcc tgactatctg cgccaggcca gagaacacgg
     1201 cgtgaagttt gtgctcttca gcccgctgcg tgatgatctg ccagacttcg tgcaggcaga
     1261 gtggcagccg ctcgtcccag gaacggatgt agccgtaatg ttggcgttgg cgcacacgct
     1321 aattacggag caactgcacg acactgcctt ccttgaccgc tactgcgttg gcttcgagcg
     1381 cttcgagcgc tatgccctgg gcgaggacga cggccagccc aagacgccgg agtgggccga
     1441 gcagatctcc gaggtgccag cggaagccat tcgcaccctg gcgcggcgca tggcaaccca
     1501 caagaccttc attactatga gctggtcctt gcagcgcacg gaatttggcg aacaggcgcc
     1561 ctggatggga gcaaccctgg ccgccatgct gggccagatc ggtctgccta gtgggggctt
     1621 tggctttggc tactcgtcca cttccatggt cggctcggcg ccgctgcgtt tcaaattccc
     1681 cagcctgccg caggggaaaa acggggtcaa aacgttcatt ccggtggcgc gcatcgctga
     1741 catgctcttg cacccaggag aagccttcga ctatgatggt caacgcctca cctatcccga
     1801 tattcggctg gtctactggt gcgggggcaa tcccttccat caccatcagc accttgcgcg
     1861 cttgcgccgc gcatttgagc gaccagatac cgtggttgtc cacgacccgt tctggactgc
     1921 tatggcccgc aacgcggaca tggtgctgcc gagcacgatg acccttgaac ggaatgacat
     1981 cggctggaac gaaaacgaca gcacgttggc tgcgatgcgg caggcggtcg catcgttcgc
     2041 gcaggcgcgc agtgactacg aaatcttcgc ggccctcgcc gccaaactcg gcttttggga
     2101 ccgcttcacc gaggggcgca acgagatgga ctggctgcgt catatgtacg acatctggcg
     2161 cactcccgcg agcgagcacg gggttgaagt gcccacgttt gaggactttt gggaagaagg
     2221 ctatctcgaa caagattgga gcgacgatca cgaggtactc ttctctgcgt tccgcgccga
     2281 cccggaacac gcgccgttgg caacgcccag tgggcgcatc gaactcttct cggagaccat
     2341 ccacagcttt ggctatgccg actgccccgg ccatccaaca tggttggagc caagtgaatg
     2401 gaccaaaggc gcgcgtgccg agcagttccc aatcctctca ttgccaacaa tcccagcacg
     2461 cgcctgcaca gccaactcga catcggcgcc acaagtcagg cgtctaaagt tcaaggcaga
     2521 gagccgattc gcctgcatcc gaccgatgca gcgacacgag ggatcgctga gggggatgtg
     2581 gtccgggtgt tcaacgatcg cggcagttgt ctcgctggcg cggtgctgag caaggacgta
     2641 cggcccaggg tggctcaact ctcgacgggc gcctggtacg acccgctcga cccggctgac
     2701 gttgatgcgc tgtgcgtgca cgggaatccc aacgtgttga cgatggacca gggcacgtct
     2761 agcctagccc agggctgcac cggccagcat gtactcgtcg aaatcgagcg ctggaatgct
     2821 cctgtgccgg agatcaccgt gtccagaccg cccccgctgg agcagcgcga cgggcttgag
     2881 gtgtcatagg gagacagcct gcttgggccg tctccctcag gggcgatgcc cctaatgtgc
     2941 ggaaattaca tccgga
//
LOCUS       DASZAK010000101         5446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_499458, whole genome shotgun sequence.
ACCESSION   DASZAK010000101 DASZAK010000000
VERSION     DASZAK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499458"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1234
                     /locus_tag="VGT44_01640"
     CDS             107..1234
                     /locus_tag="VGT44_01640"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV2579527.1"
                     /translation="MQSVLSDVVVLDLSRVLAGPYCTMMLGDLGATVIKVEQPGKGDD
                     TRAFGPPYIAGESAYYLGLNRNKYSILVDFAAPEGRERLHELIRSATVVVENFRPGTL
                     DKWGLGYEALKAANPGLIYCSISGYGQTGPYAQRPGYDFVAQAESGIMAVTGEIEGEP
                     QRVGSPIGDISAAMFATISILAALRVRDRTGQGQYIDISLLETTMSMLSNVASNHLIS
                     GEEASRFGNGHPNIVPYQAFRTSDGHVVVSCGNDRLYLALCRLLGREDLASDPRFATN
                     PQRVRNRYELVPILQEAFLKRSTGEWLPELRSVGIPCGPINSVAEIFSDPHIQARGYV
                     WECDHPTAGKIKLAGSPIHLSETPTRLYKAPPLLGEDDEKV"
     gene            complement(1249..2220)
                     /locus_tag="VGT44_01645"
     CDS             complement(1249..2220)
                     /locus_tag="VGT44_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEV2579528.1"
                     /translation="MPGILLVDPVPAPLAERLRLLFPPAAPINMVASYSEEQLIRYAL
                     ETEILLVIHRKVDAHLLSLLPRVRFIQRVGVGYDNLDLGALQAAGIVAAYTPGANAVP
                     VAEHTILLMLALLKRFVAAESAARQGNWPTAELIQAGLGDLANATVGLVGFGNIGRAV
                     AQRLAPFGTQLVYTARHAVDPAIEQQFGARYVSLDDLLASSKFVSLHLPLTATSRGLI
                     GEAELAKMQQGAFLINTSRGEILDEMALHRALVSGKLGGAALDVLSSDPPASNPFADM
                     PQVIVTPHIAGASRAGVERGVQMAIANVARFMRGESLLDLIPMPSAS"
     gene            complement(2325..3389)
                     /gene="ccsB"
                     /locus_tag="VGT44_01650"
     CDS             complement(2325..3389)
                     /gene="ccsB"
                     /locus_tag="VGT44_01650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEV2579529.1"
                     /translation="MQFLSLAATTATGASSGLAVMSNYFFSTAAVMLVVMTLVYLWYT
                     IGSARFATQVETEQIRARSGQAKKAGKVASAAAGSIGTLVKTDVVLHEQADGERPIAL
                     NRGLIAVGRVGTVVGWFSVFMFFMSLLFRAIVEQHAPWSNLYEFSLSFTTALLLCYLV
                     FEARFHERVRAWGLYVCVLSMVIVGIALYIGFAYNLINGSSQLIPALQDQPILTIHVS
                     MAIFAYALFSVAFGAGLIMLIQGGEGQRYRWLPSAEAADELAYKAVIVGFPLLALTLI
                     LGAYWANYAWGHYWSWDPKETSALVTWLLYAVYLHARGIRGWRGKRVAWLLTIGFAAT
                     LFTYYGVSFFVPSLHSYAIP"
     gene            complement(3561..4106)
                     /locus_tag="VGT44_01655"
     CDS             complement(3561..4106)
                     /locus_tag="VGT44_01655"
                     /inference="COORDINATES: protein motif:HMM:NF025908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2579530.1"
                     /translation="MQISEPAEYAILGLLEHQPMHGYEMFQQFQDGILGQVVHLEMSQ
                     MYAFLKKLERLGHIEAELEPQGTRPPRKIFHLTDQGRAVFQDWLTTPVERPREIRLLF
                     LLKLYFAQSIMPRQVSHLVELELAACQKFLAHLEAQRLPTSSSGDTAFLERVVLHSRI
                     HQTRALLNWLRELQEEFAEIR"
     gene            complement(4307..4579)
                     /locus_tag="VGT44_01660"
     CDS             complement(4307..4579)
                     /locus_tag="VGT44_01660"
                     /inference="COORDINATES: protein motif:HMM:NF022280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEV2579531.1"
                     /translation="MATIHVNTDLMRQLGQVFVQLNDQISGQVQPQIQSHTGQLEGDW
                     QGVSRQKFEQLYQDWRAAADRLVSQGEDIGRHLQNTATMFEQADQS"
     gene            4833..>5446
                     /gene="selB"
                     /locus_tag="VGT44_01665"
     CDS             4833..>5446
                     /gene="selB"
                     /locus_tag="VGT44_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEV2579532.1"
                     /translation="MSCIGTAGHIDHGKSTLVKALTGIDPDRLAEEKARGMTIDLGFA
                     WMTLPGGREVSIVDVPGHEGFIKNMLAGVGGIDAALLVIAADEGVMPQTREHLAILDL
                     LRVRHGVVALTKADLVDDEWLELVQEDIREYLKPTTLAGAAILPVSAYSGQGLPELVA
                     ELERVLNAEEERQNIARPRLPIDRVFSMTGFGTIVTGTLLDGAFR"
BASE COUNT         1183 a         1550 c         1646 g         1067 t
ORIGIN      
        1 ataggcacgt gccatattct atagtaggaa atgggagatg gcaagcagta gctcaaacgt
       61 agtatagtaa tgccaagaac tacatatatg cttcaggagg ttccctatgc aatcagtcct
      121 ttccgacgtt gtggtcctcg acttgtcgcg cgtgctggca gggccgtatt gcacgatgat
      181 gctgggcgac ctgggcgcga ccgtcatcaa ggtggagcag ccggggaagg gcgatgatac
      241 gcgcgcattc gggccgccct acatcgccgg ggagagcgct tattacctgg ggctgaaccg
      301 caacaagtac agcatcctgg tcgatttcgc tgcgccggag ggcagggaac gcctgcacga
      361 gttgattcgc tcggcgacgg tagtggtcga gaacttccga cccgggacgc ttgataagtg
      421 gggattgggc tacgaggcat tgaaggccgc caaccccggc ctgatctatt gctcgatcag
      481 cggctatgga cagaccggac cttacgcgca gcgacccggc tatgacttcg tggcgcaggc
      541 ggagtcgggc atcatggccg tcacgggcga gatcgagggc gagccgcagc gcgtcggctc
      601 gccaatcggt gatatctcgg cggccatgtt cgccaccatc tcgatcctgg cggcgctgcg
      661 cgtgcgcgac cgcaccggtc aggggcaata cattgatatc tcgctgctcg aaacgaccat
      721 gtcgatgctg agcaatgtgg cctccaacca ccttatctct ggcgaggaag cgtcgcgctt
      781 cggcaatgga catcccaaca tcgtgccata ccaggctttc cgtaccagcg atgggcatgt
      841 cgtggtctcg tgcggcaacg accggctcta cctggcgctg tgccgcctgc tgggacgcga
      901 agacctggcg agcgacccgc gtttcgccac caatccgcag cgcgtgcgca accgctacga
      961 actcgtgcca attctgcaag aagcctttct caaacgctcg accggcgaat ggctgccgga
     1021 actgcgctcc gttggcatcc cctgcggccc catcaacagc gttgccgaga tcttcagcga
     1081 cccgcatatc caggcgcgcg gctatgtctg ggaatgcgac catccaacgg cgggcaagat
     1141 caagctggcc ggttcgccta ttcacctttc ggaaacaccc acgagattat acaaagcacc
     1201 accgctgctg ggtgaggacg atgagaaggt gtaagaatgt accagccctc aagaagctga
     1261 tggcatggga atcaagtcga gtaaggactc gccgcgcatg aagcgcgcca cgtttgcgat
     1321 cgccatctgc accccccgct ctacgccagc gcgggaagcg cctgcaatgt gtggcgtgac
     1381 aatcacctgg ggcatgtctg cgaagggatt acttgccggg ggatcgctac tcaacacatc
     1441 aagggctgcg cctccgagtt tgccgcttac gagcgcgcga tggagggcca tttcgtcaag
     1501 tatctcgcca cgtgacgtgt tgatcagaaa cgctccctgc tgcatttttg ccagttcagc
     1561 ctcgccaatc aacccccgac tcgtcgcggt tagtgggagg tgcaggctga caaacttaga
     1621 ggacgccagc aagtcatcaa gcgagacata gcgcgcaccg aattgctgtt cgatcgctgg
     1681 atcgactgca tggcgggccg tatagacgag ttgcgtaccg aaaggcgcca gccgctgggc
     1741 cacagcacgc ccgatgttgc caaatccgac gaggccgacc gtagcgttgg ccaggtctcc
     1801 gagtccggcc tggatcagct ccgccgtggg ccaattgccc tgacgcgcgg cggattctgc
     1861 ggccacaaac cgcttgagca atgccagcat caataaaatc gtatgttcgg cgactggcac
     1921 tgcattggcg cccggcgtat acgccgccac gatcccggcg gcttgcagcg cgccaagatc
     1981 gaggttgtcg taaccaacgc cgacgcgctg gatgaaacgc acacgcggca agagagagag
     2041 caggtgagca tccactttcc gatggatgac gagcaaaatc tcggtctcca gagcatagcg
     2101 gatgagttgc tcttcgctat acgaagctac catgttaatt ggggcagcgg gaggaaacaa
     2161 taatcgaaga cgctcagcaa gaggagctgg gaccgggtca accagcagta taccgggcat
     2221 aacatacctc ctttacatga gagagccagc ttcgactcct tcaggagcaa tttactttat
     2281 gcttttggga ctggaaacgt cctcatcgtg ccatcaaggt tttactacgg tatggcgtaa
     2341 ctatgcaggc ttggcacgaa gaaactgaca ccatagtagg tgaagagcgt ggcggcgaag
     2401 ccgatcgtca gcagccaggc gacgcgtttg ccgcgccagc cgcgaatgcc gcgcgcgtgc
     2461 aggtagactg cgtagagcag ccatgtcacc agggcggagg tctccttcgg gtcccagctc
     2521 caatagtggc cccaggcgta gttggcccag tacgcgccca ggatcagcgt cagcgcgagc
     2581 agggggaaac cgacgatgac tgccttgtac gccagttcgt cggcagcctc agccgagggc
     2641 aaccagcgat agcgctggcc ttcgccgccc tgaatgagca tgatgaggcc cgcgccgaag
     2701 gcgacgctga acaaggcata ggcaaagatg gccatcgaaa cgtggatcgt caggatgggc
     2761 tggtcttgga gcgcggggat taactgcgac gagccgttga tgaggttgta cgcaaagccg
     2821 atatataggg cgatgccgac gatcaccatc gatagcacac agacatacag gccccaggcg
     2881 cggacgcgct cgtgaaaacg cgcttcgaag acgaggtaac acagcagcag ggccgtcgtg
     2941 aaactcaggc tgaactcgta caggttgctc cagggcgcgt gctgttcgac aatcgcgcgg
     3001 aacagcagcg acatgaagaa catgaagacg ctgaaccagc cgacgaccgt gccgacacgt
     3061 ccaacggcga tgaggccgcg attgagcgcg atcgggcgct cgccatcggc ctgctcgtgc
     3121 agcaccacgt cggtcttgac cagcgtccca atgctgccgg cagccgcgct cgcgaccttg
     3181 ccggctttct tcgcctggcc gctcctggcg cggatttgtt ccgtctccac ctgcgtcgca
     3241 aatcgcgcgg aaccaatcgt gtaccacagg tagaccagcg tcataacaac cagcatgact
     3301 gctgccgtcg agaagaaata attcgacatg accgccagtc ccgacgatgc gccggtcgcg
     3361 gttgtcgcag caagagataa gaactgcata acgaacctct attatcctga gaaatatcct
     3421 cacctgggcc gatcaaactg gccttcctta gtagatagta gcatggcctg tccagtgctg
     3481 gcaagatgac aacatgtggc atacacgctg aatttgatct tatgtaacat agaaatttac
     3541 aagaaaaaca ctacatccga ttagcgtatt tcggcgaatt cttcttgcag ttcgcgcagc
     3601 cagttcagca gggcgcgcgt ctggtggatg cggctgtgca gtacgactcg ttcgagaaag
     3661 gcggtatcac cggaagacga ggtcgggagg cgctgcgcct cgagatgtgc gaggaatttc
     3721 tggcaggcgg ccaattctag ttctacgaga tgactgacct gtcgcggcat gatcgactgc
     3781 gcgaagtaga gcttgagcag gaagagcagg cggatctcgc gtgggcgttc gacgggcgtc
     3841 gtcagccaat cctgaaagac ggcgcgcccc tggtcggtga ggtgaaagat tttgcgcggt
     3901 gggcgcgtcc cctgcggctc cagttccgcc tcgatatggc cgagccgttc caacttcttg
     3961 aggaaagcgt acatctggct catttccagg tggacgacct ggcccaggat gccgtcctgg
     4021 aattgctgga acatctcata gccatgcatt ggctggtgtt cgagcagtcc caatatggcg
     4081 tattcggcgg gttcgctgat ttgcatgtga tcgatcatcc cctgcgattt ctgactgcgc
     4141 gcggtgttat tataacaagt agccagaggg atggcaagaa cggatggcca gggtgatgct
     4201 ggccatcgcc ctggaatctc cacggcaagg caaaaaacag cgcgggcgac attcccttaa
     4261 cgcatcaaag gaaggtcgcc cgcgttgacg aagcgaacgc gcaggattag gattgatcgg
     4321 cctgctcgaa cattgtggcc gtgttttgca ggtggcggcc aatatcctcg ccttgtgaca
     4381 ccaggcggtc ggcggcggcg cgccaatcct ggtacaactg ctcgaacttc tggcggctaa
     4441 caccctgcca gtcgccttcc agctggccgg tgtggctctg aatctggggc tgaacctggc
     4501 cgctgatctg atcgttcaat tgcacaaaga cctggccaag ttggcgcatc aggtcggtat
     4561 tgacatgaat agtagccatg tgcgaaatgc atctccttga gtagtgtaaa gtaaacagca
     4621 atgatgagaa acgccgtgcg ctttccgaaa tacaccaggc cgcgtcttcg tttcctcatt
     4681 tgcagtatga gcgccgctta aacaggcaat cgtcgatgta cgtgagggtc cgcaatttgt
     4741 gcaatattac cattcgagcg ggtagccgcg cgtttgccgc tgttgtatac ttatagaata
     4801 ttgttatcat cctattctca tcgtagacag atatgagttg tattggcact gccggacaca
     4861 tcgatcacgg caaatcgacg ctggtgaaag cgctgacggg tatcgacccc gaccggctgg
     4921 cggaagaaaa agcgcgcggc atgacgatcg acctgggctt tgcctggatg acgctgccgg
     4981 gcgggcgcga ggtcagcatc gtcgatgtac caggtcatga aggcttcatc aagaatatgc
     5041 tggcgggcgt gggcggcatt gacgcagctc tgctggtcat cgccgcggac gaaggtgtca
     5101 tgccacagac gcgtgagcac ctggctattc ttgacctgtt gcgcgtgcgg catggcgttg
     5161 tggccctgac gaaggccgac ctggtagacg atgaatggct cgaactcgtc caggaagata
     5221 ttagagagta cctcaagccg accacgctgg caggcgcggc cattctcccc gtttccgcct
     5281 atagcgggca gggtctgccg gaactggtcg cagagttgga gcgcgtcctg aatgccgaag
     5341 aagagcggca aaacatcgcc cgtcctcgcc tacccatcga ccgcgttttc tctatgaccg
     5401 gtttcggcac catcgtcacc ggcacactgt tggatggagc gttccg
//
LOCUS       DASZAK010000102         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_570597, whole genome shotgun sequence.
ACCESSION   DASZAK010000102 DASZAK010000000
VERSION     DASZAK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570597"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2235
                     /locus_tag="VGT44_01670"
     CDS             <1..2235
                     /locus_tag="VGT44_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2579533.1"
                     /translation="TQPSGLVSQDFLWGLLWLPALGVPYIWFAIGLYYAALINQKWRK
                     RRPYLLIFGGALGTVVLALLLFNASSFTYADALRAIAYDNDANHYVSGLLSLPVLLSL
                     LFLIYVTFCAIAPWFTASRIARLLRVLWHNLASKSLGKPASSSLRHALVDAFWDDPHD
                     LESLEEPHLSWHLARPWLLLAALLMVGLTTSISVLIIQSIVGLLRQSASSGTTHTTPP
                     NNIVLTESLFPLRLIILDLGAMVAVALVILLIGYSIVRHGILIERPLARRGFFEQWRG
                     IVIVATALAIFIALLVTQIHNNLGSLLLITSLATVAYALFTWSSYTAHDRYIALLGPF
                     VRSASLRHWLNTDLAKTERDLESLFVHLCYNVLAVQCAYLDVLTGPVRRSFSYRWSAD
                     AVPGAMVAAIKEKRRLEERNVRSEQDGSQIRELKRLEEAPSRLPVSVNGVPMICWILP
                     IHDDRGLVATLYLGPREDGSAFTDEDMNLAQACGQRILDTLGDHEAMQAVAGLLRRRV
                     VDVKLLGAQQRRILHDEILPQIHLALLGLEKLRSQPAEVETHTGEISDGEASHTREQV
                     IEDTIDLISDTHRRLAAMMRATASGAPHHLEHDGMIAAIRAMLDQDFQHAFDEIEWLV
                     SEETAAYIDEVTPPAIAELVFAAVQEALRNAARHARGADEHRLLRLLFKASWNATENC
                     LEVIVADDGVGAGSAKSTTSGTGGGLLTHSALLAIAGGSLTVKSSPGEGVMVRILLPA
                     ESLR"
     gene            2490..>3248
                     /locus_tag="VGT44_01675"
     CDS             2490..>3248
                     /locus_tag="VGT44_01675"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV2579534.1"
                     /translation="MILITGATGYIGRHLVARLVEQGERPRCLVRDIGRAGPVLSAAQ
                     VDLMQGDTTQPGTLRRAMQGIDTVIHTAFITADRKQSAGNHYNETNVDGTANVVKAAR
                     ATGVKRIIVLSGLGTKPDKPGSYMQGRYQAEQMVKESGMDWTIIQPSVLFGKDAPFIK
                     GLADLIRTSPVIPLIGGGKIMFQPIYVEDVVSVIIDVLNQPERSANKTFTIGGPAYYS
                     FTEVVTMLANAMHKKRLMAPAPRPLVGIGAAVMEA"
BASE COUNT          653 a         1023 c          919 g          653 t
ORIGIN      
        1 acacaacctt ccgggctggt cagccaggat ttcctctggg gtctgctctg gctacccgcg
       61 ctaggcgtcc cctacatctg gttcgccatc ggtctctact acgccgcact catcaaccag
      121 aagtggcgca aacggcgacc ctacctgctg atctttggcg gcgcgctggg aacggtggtg
      181 ctggcattac tgctcttcaa cgcatcgagc tttacctatg ccgacgcatt gcgcgccatc
      241 gcttacgaca acgacgccaa ccattacgtc agcgggctgc tctctttgcc tgtactgctc
      301 tccctgctct ttctgatcta cgtcaccttc tgcgccatag ccccctggtt taccgcctca
      361 cgcatcgcga gattattgcg tgttctctgg cacaacctgg cgagtaaatc attaggaaag
      421 ccggcgtcga gctcattgcg acacgcgctg gtcgacgcat tctgggacga cccgcatgat
      481 ctcgaatcgt tagaagagcc gcacctgtcc tggcacctgg cgcgcccctg gctgctgctg
      541 gccgcgctct tgatggtcgg cctcaccacc tcaatcagcg tgctaattat tcaatctatc
      601 gtcggcttac tgcgccaatc cgcttcgtcc ggcaccacac acaccacccc ccccaacaac
      661 atcgttttga ctgaaagcct gttccccctg cgtctcatca tcctggacct gggagcgatg
      721 gtggcggttg cgctggtgat cctgctgatc gggtattcga ttgtgcggca cggtatcttg
      781 atcgagcgac cactggcgcg gcgcggcttc ttcgagcagt ggcgcggcat cgtcattgtg
      841 gcaacagccc tggccatctt tatcgcgctg ctcgtcacgc agatccacaa caacctgggc
      901 agcctcctgc ttatcacttc gctggcaacg gttgcttacg cgctcttcac ctggagcagc
      961 tatacggcgc atgaccgcta catcgcgctg ctggggcctt ttgtgcgcag cgccagcctg
     1021 cggcactggc tcaacaccga cctggccaaa accgagcgcg acctggaatc gctctttgtt
     1081 cacctgtgtt acaacgtcct ggccgtccag tgcgcctatc tcgacgtgct gaccggtccc
     1141 gtgcggcgca gcttcagtta tcgctggtcg gctgatgccg tccccggcgc gatggtcgcg
     1201 gccatcaaag agaagcgcag gctggaagag cgcaacgtgc gcagcgagca agatggcagt
     1261 cagatacgag aactcaagcg gttggaagag gctccgtcgc gcctgccggt gagcgtcaac
     1321 ggcgtcccga tgatctgctg gattctaccc attcacgacg atcgtgggct ggttgccaca
     1381 ctctacctcg gcccgcgcga agatgggagc gcgtttaccg atgaagacat gaacctggcc
     1441 caggcgtgcg ggcagcgcat cctggacaca ctgggcgatc atgaagcgat gcaggccgtc
     1501 gccgggctgc tgcgccgccg cgtcgtcgat gtcaagctgc tcggcgcgca acaacggcgc
     1561 atcctgcacg atgaaatcct gccgcaaatc cacctggcgc tcctcggcct ggaaaagctg
     1621 cgttctcagc cagcggaggt tgagacgcat accggcgaga tatcggatgg cgaggcaagt
     1681 cacacgcgcg agcaggtgat cgaagatacc atcgacctca tctctgacac gcaccgccgc
     1741 ctggccgcca tgatgcgcgc caccgcgtct ggcgctcccc atcacctgga acacgacggc
     1801 atgatcgccg ctatacgcgc catgctcgac caggacttcc agcatgcctt cgatgaaatc
     1861 gagtggctcg tgtccgagga aacagcggcc tacatcgacg aagtgacgcc gcccgccatc
     1921 gccgagctgg tcttcgccgc cgtgcaggag gcgctgcgca acgccgcccg ccacgcgcgc
     1981 ggcgcggacg agcatcgcct cctgcgcctg ctgttcaaag cctcgtggaa cgccaccgag
     2041 aactgccttg aagtgattgt ggccgacgat ggcgtgggcg ctggctccgc caagagtact
     2101 actagcggca ccggcggagg tttattgaca cacagtgcgc tcctggccat cgccggcggc
     2161 agcctcaccg tcaaaagctc acctggagag ggtgtgatgg tgaggatact gctgccagcc
     2221 gagtcattga ggtagcatgt gcctgctcac cttttctttc atgatgaaac ccgctcgcgt
     2281 aacttatatc agttgcctat acttattagt aatgatgatg aagtttcaca accaccatcg
     2341 ggaatgtcat ccttcgctgc gctcaggatc tcggtgttcg gtgagtgaga tccttcgctg
     2401 cgctcaggat gacacgagta ttaccgacct tgattgtcaa aattcataat caaacaaaga
     2461 cacaacacaa attatcatag gagaaatcga tgatactcat taccggagct acaggatata
     2521 ttggcaggca tctcgtcgcg aggctggttg agcagggaga acggccacgc tgcctcgtgc
     2581 gtgacattgg gcgagcgggg ccagtactct cagccgcgca ggttgatctg atgcaagggg
     2641 acacaacgca gcccggcaca ctgcgccggg ccatgcaggg gatagatacg gtcattcaca
     2701 ccgccttcat caccgcagat cgcaagcaat cggcgggcaa tcattacaac gagacgaatg
     2761 tggatggtac cgcgaatgtc gtcaaggcag ccagagcgac gggcgtcaag cgcatcatcg
     2821 tcctcagcgg cctgggaacg aagcccgata agccgggcag ttacatgcag gggcgctacc
     2881 aggcggagca gatggtcaaa gagagcggca tggactggac gattatccag ccatcggtgc
     2941 tattcggaaa agatgcgccc ttcatcaaag ggctggcaga cctcattcgc acatcgccgg
     3001 tcattcccct cattggcggc ggcaagatca tgttccagcc catctatgtc gaggatgtgg
     3061 tctcggtgat cattgatgta ttaaatcagc ccgaaaggag cgccaacaag accttcacca
     3121 tcggcggccc cgcgtattat tccttcaccg aggtggtgac gatgctggcc aacgccatgc
     3181 acaaaaaacg cctcatggct cccgctcctc gcccactcgt cggcattggg gccgccgtga
     3241 tggaagcc
//
LOCUS       DASZAK010000103         4210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_215484, whole genome shotgun sequence.
ACCESSION   DASZAK010000103 DASZAK010000000
VERSION     DASZAK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215484"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VGT44_01680"
     CDS             <1..311
                     /locus_tag="VGT44_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HEV2579535.1"
                     /translation="WIQAERDRLQQMFQEALERLIELLEHERNYAGAIRVAQRLLRVD
                     PLQEPTYQRLMRLYAARGDRGAISRTYQACAAVLKRELAIQPGLTTRKTYEQLMQAED
                     "
     gene            330..557
                     /locus_tag="VGT44_01685"
     CDS             330..557
                     /locus_tag="VGT44_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579536.1"
                     /translation="MNPQMQTQVQPELSTYANDALSETVMLAEWGFDSYEITSLLWLR
                     QWYQTGGSDRASMIRHLEFLRMLVQSGEMEL"
     gene            622..1143
                     /locus_tag="VGT44_01690"
     CDS             622..1143
                     /locus_tag="VGT44_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579537.1"
                     /translation="MSGNENPIVVTNNPGESGPAIALSAGLAALTVGEAIFLLAALIL
                     SPSAVQNDIFLAYSLLGQALIAIGALLVAGGLIGLSAAHWLVALCVLAVAGFAAYGFH
                     WPDALLPAATNPFRPFIGQFTLSGLMLVLFLGALLSHRQQVWPSLWRPLLVAAVIAAV
                     AIAVVAWLYSRIA"
     gene            1292..2713
                     /locus_tag="VGT44_01695"
     CDS             1292..2713
                     /locus_tag="VGT44_01695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579538.1"
                     /translation="MSRTRPLPLETHEKGALIPEGIWSPRHRRLTLGLILLVSGPAFE
                     GLAVATILPRVVANLGGLSLYGWSFSAYMLANILGLILAGDEADRKGPALPFIFGVSL
                     FVPGLILAGIAPSMLIFVLSRGVQGLGAGIIASVAYVCLGRGYPEPLKPQMIAILSSA
                     WVVPGLVGPALAGIVADAFGWRWVFLGLVPVLPLATILILPALKILIPPTSSGSWDLH
                     RLLAALGITVGAGMTLAGLQAPSLPLALALTLSGLVIGIFSLYRILPAGTLKAKAGMP
                     AAIATMGLLSIGFFGGDAFLPLTIISIRGQSTLVAGVALTAATLTWTAGAWIQAKLAS
                     RQGRRLLVTSGLILIALGLAGITCILIPGVPVVLAIVSWGVAGLGMGLAYSTVSLVVL
                     ETAPPDQQGSATASMELASTLGSALGAGLGGAIIAIATASGNGIGTGISVVDLLVIAV
                     IGIAILAAVRLPGRPHQAPSLPL"
     gene            2846..3304
                     /locus_tag="VGT44_01700"
     CDS             2846..3304
                     /locus_tag="VGT44_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579539.1"
                     /translation="MSVPTYAEPQQQSRRSRRTFWIIAGSILAIALIGLAGYVAVLTS
                     SALNSLKQTGSIPERYYLNIMSGDYTTASTYLDSNATIDGQPVDQQSFMRLAKATDAQ
                     YGGVRGITFNIESDATHVTVTVSRGSRSYDVHLVLQQENGAWKIVSADGI"
     gene            3339..>4210
                     /locus_tag="VGT44_01705"
     CDS             3339..>4210
                     /locus_tag="VGT44_01705"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEV2579540.1"
                     /translation="MSTIATGSQSATSSSLKRIISSHPLLAYFVIAFAGTWLLELPAI
                     LGKNGLALLPFTVPFALFAVLFILSSFAGPTLGAFLVTAITGGKPGVRQFLRRYVQWR
                     VGLLWYLVVLFGYPVVYLLTTSIFLGVAPFSALIQNWPLLFTSYLPAILLFPGIITLG
                     EEPGWRGFALPRLQQAYGPITGSLILGALHGLWHLPIFLIASFGNGPFVSPLPYLINV
                     LQIMVITLIWTWIFNKARGSILIAFLNHSAFNANGSLFNTLVPVMLLGGRAPIYALAI
                     VALLIIILTRGRLG"
BASE COUNT          802 a         1283 c         1175 g          950 t
ORIGIN      
        1 agtggataca ggccgagcgc gaccgcttgc agcagatgtt ccaggaagca ttggaacgcc
       61 tgatcgagct gctcgaacac gaacgcaact atgccggggc gatccgcgtg gcgcagcgcc
      121 tgctgcgggt agatccgctg caagagccca cctatcagcg cctgatgcgc ctgtacgcgg
      181 cgcgcggcga tcgcggagca atctcgcgca cctatcaggc ctgcgccgcc gtcctgaaac
      241 gcgaattggc gattcaacca gggctgacta cccgcaagac ctacgaacag ctcatgcaag
      301 cagaagatta atcgagaaag gacaggacca tgaacccgca gatgcagacg caagtacaac
      361 ccgaactgtc aacgtatgcg aacgacgctt tgagcgaaac agtgatgctg gccgagtggg
      421 gtttcgactc gtatgaaatc acctccctgc tctggctgcg gcagtggtac cagacgggcg
      481 gcagcgatcg cgccagcatg atacgccacc tggaattcct gcgtatgctg gttcaaagcg
      541 gtgagatgga attgtaacag aaagaaaatg caaccggcgc aggccacgcg gtaaaataac
      601 atagtataag gagcagccag catgtcaggc aatgaaaatc caattgtcgt caccaacaat
      661 cctggggaga gcggcccggc catcgccctc tcagcaggac ttgctgcact gacggttgga
      721 gaagccatct tcctgctggc tgcgctcatt ttatcgccat cggcagtaca aaatgacatt
      781 tttttagcgt atagcctgct gggccaggct ctgatcgcca tcggggcgct gctggtcgcg
      841 ggcggattga tcggcctcag tgccgcgcac tggctcgtgg cgctgtgcgt actggcggta
      901 gccgggttcg cggcctatgg ctttcactgg ccggacgcgc ttcttcccgc cgccactaat
      961 ccatttcgcc ccttcatcgg gcagttcacc ctgagcggcc tcatgctggt gctgtttctg
     1021 ggagcgctcc tcagccaccg gcaacaggtc tggccgtcgc tctggcggcc cctgctggta
     1081 gctgcggtga ttgcagccgt cgcgatcgcg gtggtggcct ggttatacag ccgtattgct
     1141 taatccggcc ctatcatcat ccagccctgt catcctgagc gcagcgaagg atctcggtat
     1201 tcgatgaatc agatccttcg ctgcgctcag gatgacaggg ccgaagtcgg ttgtccaatt
     1261 tcatcattag aaagcaagaa agaaacgttc tgtgtcgcga acgagacctt taccgctaga
     1321 aacgcatgaa aaaggcgcgc tgattcccga agggatctgg agtccgcggc accggcggct
     1381 tacgctggga ctcatcctgc tcgtatcagg gccagccttt gagggattgg cggtcgcgac
     1441 catactgccc agggtcgtcg cgaaccttgg gggactgagc ttgtatggct ggtcgttcag
     1501 cgcctatatg ctcgcgaata tcctcggcct cattctggcc ggtgatgaag ccgaccgcaa
     1561 agggcctgcg ctcccgttca tcttcggcgt gtcgctcttc gtgcctggtc tgatcctggc
     1621 gggtattgcc ccatcgatgc tcatcttcgt cctgagccgg ggggtgcagg gtctgggagc
     1681 gggcatcatc gcctctgtgg cctacgtctg cctcgggcgc ggctatccag aaccattgaa
     1741 accgcaaatg atcgctattc tttcgagcgc ctgggtcgtg ccagggctgg ttggtcccgc
     1801 gctcgcaggc atcgttgccg atgctttcgg ctggcgctgg gtcttcctgg gcctcgtccc
     1861 ggtattgccg ctcgccacca tactgatact ccctgccctg aaaatcctga ttcctccgac
     1921 atcctctgga tcatgggacc tgcatcgtct tttggccgcg ctcggcatta ccgttggagc
     1981 aggcatgacc ctggcagggt tgcaggcgcc atctcttcca ctcgcccttg cgctgacact
     2041 ctcaggtctg gtgataggta tcttttctct gtatcgcata ctcccggcgg gaacgctgaa
     2101 agcgaaagcc gggatgcccg ccgctatcgc cacgatgggt ttgctcagca taggattctt
     2161 tgggggagat gcgtttctcc ccttgacgat catctccatt cgcggtcaaa gtacgctggt
     2221 cgccggggta gcgctcaccg ccgccacgct gacctggact gccggagcgt ggattcaggc
     2281 aaagctggcg tcccggcaag gacgccgcct gctcgtcacc tccggtctca tcctgattgc
     2341 gctgggactg gccggtatca cctgtatcct cattccgggc gttccagtcg tcctggcaat
     2401 tgtctcctgg ggagtcgccg gtctgggcat gggcctggcg tattctacgg tcagtctcgt
     2461 tgtcctggaa acggctcctc ccgaccagca agggtcggca acagcatcga tggagcttgc
     2521 ttccacgctc ggctcagcgc ttggcgcggg tctgggagga gcgatcatcg ccattgcgac
     2581 ggcttccggg aacgggatcg gcacaggcat cagcgtagta gacctgctcg tcatcgccgt
     2641 aattggcatc gccatcctgg ccgccgttcg actgccaggg cgaccgcacc aagctccatc
     2701 tctccctctg tgatctggtt ctaactttct ctacaggaaa cgcttcaaga aacgtttgag
     2761 aaactctcca ccgtctacac ttctcttgtt gcaacacgcg acagtgataa tcccaggcat
     2821 accctggtga gaaaggaaca ccatcatgtc tgtaccaacc tacgcggagc cacagcagca
     2881 gtcccggagg tcccgccgta cattctggat cattgctgga agtatcctgg ccatcgctct
     2941 gatcggcctg gccggctatg tagctgtgct tacctcctcg gcactgaatt ccctcaagca
     3001 aaccgggtcg atccctgagc gctactacct gaacatcatg agcggcgact ataccacggc
     3061 ctctacctac ctggatagta atgcaaccat cgatggacag ccggtggatc agcagtcgtt
     3121 catgcgcctg gcaaaagcca ccgacgcgca atacggcggt gtgcgcggca tcacctttaa
     3181 catcgagagc gacgcaacgc atgtcaccgt gacggtctct cgcggctcgc gcagctacga
     3241 cgtacacctc gtcttgcagc aggagaacgg cgcctggaag atcgtcagcg ccgacggcat
     3301 ttagcaacaa tcgcatctag cgagaaggga aactcatcat gtctaccatc gcaacggggt
     3361 cgcaatctgc aacctcctca tcgctcaaac gtattatcag tagtcaccct ttgctggcct
     3421 acttcgtcat cgcgttcgct gggacctggc tgctcgaact tccggcgatt ctgggcaaaa
     3481 atggcctggc actgctcccg ttcaccgtac catttgcatt gtttgctgta ctctttatac
     3541 tgagttcttt tgccgggccg acactgggag ccttcctggt gacagctatc acgggtggaa
     3601 agcctggagt aaggcaattc ttgcgccgtt acgtgcagtg gcgcgtgggt ctactatggt
     3661 acctggttgt gctgttcggc taccccgttg tctacttact gactacgagc attttcctgg
     3721 gagtggcgcc gttctcagcc ctcatacaga actggccact gctcttcacc tcgtacctgc
     3781 cagccatcct gctgtttccc ggtatcatca ctctgggaga agaacccggt tggcggggat
     3841 ttgcgctgcc gcgcttacaa caagcgtacg gtccaatcac cgggagcctc attcttggtg
     3901 cgctgcatgg tctgtggcac ctgcccatct tcttgatcgc ctcattcgga aatggaccat
     3961 tcgtcagtcc actcccctac ttgatcaacg ttctgcaaat catggtgatc actctcatct
     4021 ggacgtggat cttcaacaag gcgagaggca gcatcctcat tgcgtttctc aaccactccg
     4081 ctttcaatgc gaatggatcc ttgttcaaca ctcttgtacc cgtaatgctc ctgggcggcc
     4141 gggcacccat ctatgcatta gccatagttg ccttgcttat catcattctg accaggggcc
     4201 gcctggggta
//
LOCUS       DASZAK010000104         5306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_570608, whole genome shotgun sequence.
ACCESSION   DASZAK010000104 DASZAK010000000
VERSION     DASZAK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570608"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /locus_tag="VGT44_01710"
     CDS             complement(<1..1025)
                     /locus_tag="VGT44_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2579541.1"
                     /translation="MTPRALFAASSRMKSTSMIFTLLVAFTLILSACGGGTSTTNNGN
                     TKHILKIAAQSYDFAQAGFNPYNGHPNAGVLGLVYETLYFVNVNDGSFTPMLASSYSW
                     NSTNTQVTFAIRQGVKWNDGQPFTANDVAFTFNMMKQYPTADGLGVWNFLSSVTAPDA
                     NTVVMTFQKAYPPVLVNIAGHVYIVPQHIFQNAGDPTQYLTDHPVGTGPFTLASYQPS
                     LAVYDKNPSYWQASVVKVDEIRFPEYKDNATLELALPKGDVDWAGYFSPTLQTDFLAK
                     DPTHNHIFMDAINLYSICVNQKDPILGGQSGAAIRQALSFALDRNAIAAQATAGLEPP
                     GSITGLVL"
     gene            complement(1351..2601)
                     /gene="nagA"
                     /locus_tag="VGT44_01715"
     CDS             complement(1351..2601)
                     /gene="nagA"
                     /locus_tag="VGT44_01715"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV2579542.1"
                     /translation="MRFTLRNAHLVDAARDISRGEIVIDGARIQSVAHSDDMGKAAED
                     LSEQGGQDGGEIAIDADGMIVMPGFIDVHTHGGGGFNLHTTDAGEIRSYANWSPSTGV
                     TSFLIAVVGVPGAMPEEQLKAAVEAMEARGDGVKGAEPLGIHLEGPYISAARRGAHPT
                     SWLRIPDEAETERLLATANGYLRLMTLAPELPGGLAMITRLVEAGVTVSMGHTDATYE
                     QAQEAIRLGVTHVTHCYNAMRPLLHREPGPLAALAQATGVFGELIADGVHVHPAAADA
                     LVKLLGPERTIVITDALAGAGLSDVAFEFAGQPARVIRGAARLIDGTITGSVLTMDQA
                     LRNVLQMTSVTLPQAVGMLSLNPARTLGVAHRKGCLQAGYDADVLIFDQSLALQATIC
                     RGNVAFATDAWRERLLPISTLYAV"
     gene            2829..3575
                     /locus_tag="VGT44_01720"
     CDS             2829..3575
                     /locus_tag="VGT44_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV2579543.1"
                     /translation="MGAIYRNSPLPRYFQLKEILRERIRLGEWNAGDLIPSERELSEK
                     YGISRMTARQAITDLVNEGLFYREQGKGTFVSQRKITQQLIHLTGFTEDIRARGQRPG
                     TKVLSAAMHPADEATAEKLRIAPGTLIFRLQRLRLANDEPLAIELSHIHFRECERLGE
                     EDLEQNSLYRLLESKYGIPLMEADQELEAGLAGPEDAHILKIAIGNPVLFTRRVTYTE
                     RNQPIEYARAVYCGNKYIFYTHMKREQLVP"
     gene            3746..4651
                     /gene="murQ"
                     /locus_tag="VGT44_01725"
     CDS             3746..4651
                     /gene="murQ"
                     /locus_tag="VGT44_01725"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEV2579544.1"
                     /translation="MHHPLNILETEQANPTSASIDRMTPLEIARVMNAEDAKVAQAVE
                     QALPDVARAIEAIAARLRQGGRLIYFGAGTSGRLGALDASECPPTFNVSPATVVACIA
                     GGPQAIDTAHEDQEDSAEAGHADAERLNITSADAVVGITASGRTPYVRGALACAKEHG
                     ALTIGLACNAHTPLEPEVDVMIAPLVGPEVISGSTRLKAGTAQKMVINMLSTGAMILR
                     GKTFGNLMVDVQATNYKLQQRALTIVQQATGLDAEAATRLLATSDGEVKTAILCARAN
                     LPPDQARAQLAVHGGILRDALDDMA"
     gene            4690..>5306
                     /locus_tag="VGT44_01730"
     CDS             4690..>5306
                     /locus_tag="VGT44_01730"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV2579545.1"
                     /translation="MTSYTDDMTLEEQIGQTLMVGFQGTTSPPHIVELIERFHVGNII
                     LFSHNIRDAAQVLQLTQQLQAIAREAGHRFPLLIAIDQENGIVQRLGEAATIFPGNMA
                     LGAIGSEEMAFEVARATGEELRALGINMNLAPVVDVSNNPANPVIGVRSFGEDAQLVA
                     RLGSAMTRGYHAAGVMACLKHFPGHGDTSTDSHLALPTILHSLER"
BASE COUNT         1187 a         1594 c         1481 g         1044 t
ORIGIN      
        1 agcaccaggc cggtaatgct gccgggaggc tcaaggccgg cagtcgcctg tgccgcgatt
       61 gcgttgcggt caagcgcaaa gctgagcgcc tggcgaatcg ctgccccgga ctgtccgcca
      121 agaatggggt ccttctggtt cacacagatc gagtacaggt tgatggcatc catgaagatg
      181 tgattgtgtg tcgggtcttt cgcgaggaag tccgtctgga gcgtcggcga gaagtaaccg
      241 gcccaatcaa catcgccctt cggcagtgcc agttccagcg tggcattgtc cttgtactcc
      301 gggaagcgaa tctcatcaac cttcacaaca ctggcctgcc agtagcttgg gttcttgtca
      361 tagaccgcca ggctcggctg atacgaagcc aacgtgaacg ggccggttcc gaccgggtga
      421 tcggtgagat actgggtcgg gtcgccagca ttctggaata tgtgctgcgg cacgatgtac
      481 acgtgcccgg caatgttgac cagcacggga ggatacgcct tctggaaggt catcacgacc
      541 gtgttggcat caggcgcggt cacactggag aggaagttcc atacgcccag cccatcagcc
      601 gtggggtact gcttcatcat gttgaaggta aacgccacgt cgtttgcggt gaacggctgc
      661 ccatcgttcc actttacacc ctggcgaatt gcaaatgtca cctgggtatt ggtgctattc
      721 cacgaataac tactcgccaa catgggagta aaggagccgt cattcacatt cacaaagtac
      781 aatgtctcgt acaccaggcc aagtacaccc gcgttggggt ggccgttgta ggggttaaag
      841 ccagcctgcg caaaatcgta agactgcgca gcaatcttga gtatatgttt ggtgttaccg
      901 ttgttggtgg tgctggtgcc accaccgcaa gccgaaagga tcaacgtaaa ggcaaccagc
      961 agggtgaaaa tcatcgatgt agacttcatc cttgaagatg ccgcgaaaag cgccctggga
     1021 gtcatgcata acctcctgcc gtccgatcaa cggatctgtt caacgatcca ggaaatattc
     1081 tgggatggta caattgctca accgtacacg tggaacactc gaatcgccgt caatgttctc
     1141 tggcttccct cctttccggc tctctaagtg tgtgcgaacc atcgcaaaga cgtatttcgt
     1201 tcgttatggg atttccagtg tgactctgtg aaatactgta ccttgttctg gttcatttgt
     1261 caagacatct agaccactat acaatagcaa aagaaaaatc cgataggtca ggttaacagg
     1321 tgagggcgtc tcaacctgga tggcccgtct tcacacggca tacagggtgg aaatcggtag
     1381 gagacgttcg cgccaggcat cggtggcaaa cgcgacgttg ccacggcaga tggtggcttg
     1441 cagcgccagt gattgatcga atatcaacac atcagcatca taaccggcct gcaggcagcc
     1501 tttgcggtgc gccacgccca gcgtgcgagc cgggttcagc gagagcatcc caacggcctg
     1561 cgggagtgtc acactcgtca tctggagtac attgcgcagc gcctggtcca tcgtcagcac
     1621 gctgccggtg atggtgccat cgatcagtcg cgccgcgccg cgaataacgc gcgcgggttg
     1681 gcccgcaaat tcgaaagcaa catcgctcag accggcgccc gccagcgcat ccgtaataac
     1741 gatcgttcgc tccggcccca acagcttcac cagcgcatcc gccgccgccg ggtggacatg
     1801 caccccatcg gcgatcaact caccgaacac gcctgtagcc tgggccagcg cggcaagcgg
     1861 tcccggttcg cggtgcagca gcgggcgcat ggcattgtag caatgggtaa catgcgtgac
     1921 gcctagccgg atggcttcct gcgcctgttc gtaggtcgca tcggtatgac ccatgctgac
     1981 cgtgacacct gcctccacca gccgggtaat catagccaac ccgcccggca gttcgggagc
     2041 aagcgtcatt aagcgcaaat aaccgttcgc agtcgcgagc aatcgctccg tctccgcctc
     2101 gtccggtata cgcagccagg aagtcgggtg cgcgccccgc cgcgcagcgc tgatatacgg
     2161 cccttctagg tgaataccga gcggctccgc tccttttact ccatctcctc gcgcctccat
     2221 cgcctccacc gccgccttga gctgctcctc cggcatcgcg cccggcacgc caactacggc
     2281 tataagaaac gaggtaacgc ctgtgctggg cgaccagttc gcgtaagagc gtatttcccc
     2341 tgcatcggtc gtatgcaggt taaagccgcc gccgccatgt gtatgcacgt cgataaagcc
     2401 cggcatcaca atcatcccat ccgcatcgat agcaatctcg cctccatcct ggccgccctg
     2461 ctcactcaga tcttcggctg ccttccccat atcatcacta tgcgctaccg actggatgcg
     2521 cgcgccatcg atgacgatct cgccgcgaga aatatccctt gccgcgtcaa cgagatgagc
     2581 attacgtaat gtaaaccgca ttaagcctcc tatcggaata agggctgtcc tgaacacttg
     2641 acagacggac aaaagtatgt gattattata cttaagtcca gtggtatata caacatgcca
     2701 attataccac ttgcattatt tagtaccata ttttagcaag gaaagcaagg ggctggcata
     2761 gcaaataggc gtacaatgtg tattacttac cgtgtataaa cgttgtcacg cattgttgag
     2821 gaaaaggtat gggcgcaatt tacagaaaca gtcctctgcc tcgctacttt cagttgaaag
     2881 aaatcctacg ggaacgcatc cgcctgggcg aatggaacgc gggcgatctt attccgtcgg
     2941 agcgagagtt gagcgaaaag tacgggatca gccgtatgac ggctcgccag gccatcaccg
     3001 acctggtgaa tgaagggtta ttttatcgcg agcagggcaa aggcactttt gtgagccagc
     3061 gcaagatcac ccagcagttg atccacctta ccggctttac ggaggatatc cgcgcgcgcg
     3121 ggcaacgccc cggcacaaaa gtgctgtcgg ctgccatgca tcccgcggac gaggccaccg
     3181 ccgagaagct gcgcattgcg ccgggaacgc tcatcttccg cctgcaacgc ctgcgcctgg
     3241 ctaatgatga accgctcgcc atcgaactct cgcatattca cttcagggag tgcgagcgcc
     3301 tgggcgaaga ggacctggaa cagaactcgc tctaccgcct gctcgaatcg aagtatggca
     3361 tccccctcat ggaggccgac caggaactcg aagcaggcct ggccggaccc gaagatgccc
     3421 atatcttgaa aatcgctata ggcaacccgg tgcttttcac gcgccgtgtg acctatactg
     3481 agcgcaacca gcccatcgag tacgccagag ccgtctattg tggaaacaag tacatcttct
     3541 atacccacat gaaacgcgaa cagttggtcc cgtagaaccc gtaggggtgg ttttgaagtt
     3601 tcacaaccga gatcagtcat gtcatcctga gcgcagcgaa ggatctcggt ccccggccag
     3661 ttagaccctt cgctgcgctc aggatgacat gaccggcttt ggtcgtgtaa tttcattatc
     3721 gccaggtagt tcagaggagg catccatgca ccacccgttg aacatccttg aaaccgaaca
     3781 ggccaatccc acaagcgcct ctatagaccg catgacgccg ctcgaaattg cgcgggtcat
     3841 gaacgccgag gatgcaaagg tggcgcaggc ggtcgagcag gcgctgcccg acgtagccag
     3901 ggccatcgaa gcgatcgcgg cgcggctgcg gcagggcggg cggctgatct actttggcgc
     3961 gggaacctct ggccggctcg gcgcgctgga cgcctccgaa tgcccgccaa ccttcaacgt
     4021 ctcaccggca acggtggtag catgtatagc tggcggcccg caggccatag acaccgcaca
     4081 cgaagatcag gaggacagcg cggaagcagg acacgccgat gcggagcgtc tcaacattac
     4141 cagcgcagac gccgtcgtcg gcattaccgc cagtgggcgc accccctatg tgcgcggtgc
     4201 gctggcctgc gcaaaggaac acggagcgtt gacgattggc ctggcctgta acgcacacac
     4261 gccgcttgaa ccggaggtcg atgtaatgat cgcgcccctc gtcggaccgg aggtcatcag
     4321 cggctcaaca cgcttgaaag caggcacagc ccagaaaatg gtcatcaaca tgctcagcac
     4381 cggcgcgatg atcctacgag ggaaaacctt cggcaacctg atggtcgatg tgcaggctac
     4441 caactacaaa ctccaacagc gcgccctcac catcgtgcaa caggcgaccg gcctggacgc
     4501 cgaagccgcc accagattgc tggcaacctc agatggcgaa gtcaaaaccg ccatcctctg
     4561 cgcccgcgcc aatctcccgc ccgatcaggc gcgcgcgcag cttgctgtac acggcggtat
     4621 cttgcgtgat gcgctagatg atatggcata atccctacac accaactatt gtatgagagg
     4681 aacaacgatg tgaccagcta caccgacgac atgaccctgg aagagcaaat cggtcagacc
     4741 ctcatggtcg gcttccaggg aacaacatct ccgccgcaca tcgtcgaatt gattgagcgc
     4801 ttccacgtgg gaaatatcat cctcttctcg cacaatattc gcgatgccgc ccaggtgttg
     4861 cagttgacgc agcaattgca ggccatcgcg cgcgaagccg ggcatcgctt tccgctgctg
     4921 atcgccattg accaggaaaa cggcatagtt cagcggctgg gcgaagcagc aaccatcttt
     4981 ccgggcaaca tggcgctggg cgctatcggc tcagaggaga tggcgtttga agtggcgcgg
     5041 gcgacgggtg aggaattgcg cgcgctcggc atcaacatga acctcgcacc ggtggtggat
     5101 gtcagcaaca acccggccaa cccggtgatc ggcgtgcgtt cattcggcga ggatgcgcaa
     5161 ctggtcgcgc ggctaggctc cgccatgacg cgcggctatc acgcggcggg cgtcatggcg
     5221 tgtctcaagc atttccccgg acatggcgac acatctaccg attcgcacct ggcgctgcct
     5281 actatcctcc actcccttga acgatt
//
LOCUS       DASZAK010000105         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_357674, whole genome shotgun sequence.
ACCESSION   DASZAK010000105 DASZAK010000000
VERSION     DASZAK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357674"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..323)
                     /locus_tag="VGT44_01735"
     CDS             complement(51..323)
                     /locus_tag="VGT44_01735"
                     /inference="COORDINATES: protein motif:HMM:NF017038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEV2579546.1"
                     /translation="MQPIEERLDLVRFKVDKTPHIRVNTATCMDCDTTPCVYVCPAGL
                     FELLGGEMNFSYEHCLECGTCYVACERKAISWEYPRGGWGVSFRAS"
     gene            complement(336..1658)
                     /locus_tag="VGT44_01740"
                     /pseudo
     CDS             complement(336..1658)
                     /locus_tag="VGT44_01740"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
BASE COUNT          326 a          531 c          598 g          364 t
ORIGIN      
        1 tggagaccct tgcggtcgcc ctcgtcttcc aggcctgggg tcacattcca ttatgatgcg
       61 cggaagctga cgccccagcc gccgcgcggg tattcccacg agattgcttt gcgctcgcag
      121 gcgacgtagc atgtgccgca ttcaaggcag tgttcgtagg agaagttcat ttcgccgccg
      181 agcaactcga agaggccagc cgggcaaacg tagacgcatg gcgtggtatc gcaatccatg
      241 caggttgcgg tgttcacgcg aatgtgcggc gttttatcga ccttgaaacg cacaagatcg
      301 aggcgttcct cgattggctg cattgttaca ctccttcaaa acaacagcgc gcgggcggct
      361 tgccagccgt cgcgcagcat ggtgcgcaac ggcacctttt tatgcatggc gctgtagagg
      421 gtgcggccca gtttgcggcg gggcgagccg ttggaacgaa atatgttttc ggcggcctgg
      481 cagaacagtt cgggatagac gttctgcagg cgctcgttat tgacgaatgc gggtgtgcga
      541 cggaagcgct tcaggtcttg cagcacgaag ctcttttcca gacgctggcg atagcgcgac
      601 atgctctgcg cggaatagtc gcgttcggcg tgcgcctgct gcgcggtttc ggcggcggcc
      661 aggccggagg cgatggcgaa gttcatgcct tccaggaaga ggccagcggc gaagacgaag
      721 cccgccgcgt cgcccgtcac catcagcccg gcgcgtgata gctgcggcag cacggcccag
      781 ccgccttcgg ggatcatgtg cgccgaatat tcaacctgga cactgtcgcg cacgagcggg
      841 gcgacggcag ggtgctgctt gaaacggtcg agcaggtcgt aggccctggc gcgctgttcg
      901 gcgagcgagg cgatctgcgt gacgacgccg atggagagcg tgtcgcggtt ggtgtagaga
      961 aacgcgccgc ccttgacgtt gcccgacgcg aagccgacgt attcattggt catgccctcg
     1021 tcctcagcga gctggaagcg ctcctcgatg gtgtgcgcgt ccaggcgcag cacctctttg
     1081 acaccgagcg tcatgtcatg cgcgttgaac tcatgctgca aaccggcctg ttttgcgagg
     1141 aaggaattcg ccccatcggc ggcgatgacg atgggggcgc gcaggtcgcc ctcctcgcgg
     1201 cggcagcgca cgcctatgac ctgctggcca tcgtagagca gctcgtcaac aacggttgag
     1261 gtaatcagca aggctccggc ctgctcggct ttgccggcca gccagcgatc gaatttgggg
     1321 cgcaggaggg tgaagccgtt gtagggcggc tgcgcgaaat gggctgtgcg gtaatcgagc
     1381 gcgagcgccg attcctggcc gagaaaggtg ataacgcgcc gcgtcaggta gcgttcgacg
     1441 ggcgcttcct cccaataatt gggcagcagg ccggccagca gttcgggcgc gtagagcgcc
     1501 gcgccggaga cgtttttcgc gcccgcgtat tcgccgcgtt cgatcagcgc tacctgcatc
     1561 cctgcacgcg ctattgccag cgccgcgctg ctgcccgccg gccccgcgcc gacgacgatg
     1621 gcgtcgaatt gctgctggtt ggtcatgttt ttgtttacct gcttttctaa tgcgtcaaag
     1681 ttgaaatggc tgtaggtgcc ggttgaccgc gcacaccgcc gataatgaac attaacaaat
     1741 taagttggat atggtagact atcggaaacg accaatatca caggaggttt ccgatggcat
     1801 tgcctttctc cctcgcgct
//
LOCUS       DASZAK010000106         8034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_677109, whole genome shotgun sequence.
ACCESSION   DASZAK010000106 DASZAK010000000
VERSION     DASZAK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677109"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..5065
                     /locus_tag="VGT44_01745"
     CDS             32..5065
                     /locus_tag="VGT44_01745"
                     /inference="COORDINATES: protein motif:HMM:NF036757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase-like domain-containing protein"
                     /protein_id="HEV2579547.1"
                     /translation="MLQRLAITSSDDLSKLLANPAYYGGLDLSQLPVQTAQPALSFSA
                     WLQLAQIMQFKAQYPAPQGVNFLDMLAAAQTAPNQSGILTSLSTLTTWDLTDLQTVDS
                     ALALTVADYQKIATYQRLAACFASIQRLGVKAQAVIAWAQQPSSQDISTQIKQATKAK
                     YPNDQWVTIAAPLQDAIRDLKRAALVNYLIAHPDPPNGKNWADANDLFSYFLIDVEMT
                     SCQLSSRIVQATNAIQLFAQRCLMNLEPDITADVQNDPDWQQWQWMKAYRVWEANREV
                     FLYPENWILPELRKNKSSFFKDLESDLLQNQITNDNVETGMLSYLQKLNDVSRLEVSA
                     MYHDDELGILHVFARTHDVPPIYYYRYLKDGAWSGWEKINLDIKSDHLIPVVYNRKLY
                     LFWALFTPKSEKHENQPIAPAQGGQTYNTPPPPNQYWEIQLAWSYLHDGKWIAQSHSN
                     MILIHPQRVVGSQPVSPLRPQFAYTFKAMMRGDDLVINAFISPTIEFNNPIGNYNETG
                     LPWHSSEFVFNSTVTAINVVDINGFQQWIYNNYSADGRAIGTVQQQQSSLLLPLLSHY
                     NNEDLVNISTNGGQLYVLKQSSLRLDNGQLLAAAGLPYEVVVPHQDAQFDSTRPFFYQ
                     DKQRAYFITPTILYQNGSTFTPNLPASPYNAQYEVAYQFDEFYHPFTKLFIHELNKLG
                     VPGLFDRRLQTNPGAAPFPIDSFSFSSVYQPTSLVTADNDQEIVDFSPGGAYSLYNWE
                     LFFHAPLMLALRLNQNQRFEEAAKWFHYIFDPGSASSDLPPQRYWITKPFQLQTADIL
                     NQRIDNLLKLINQHDPTYEAQVTQWRNDPFDPHMIANLRPVAYMKTTVMKYLDMLIAW
                     GDNLFSQDTIETINQATQLYILAAQILGPRPQQLPALQRAADKSFNDLEKDLDAFGNA
                     MTEVENTVVISGDGSSPNPNTPQLPQLQSFYFCIPANSQLLGYWDTVADRLFKIRHCQ
                     NIQGVTQQLPLFSPPINPALLVQAAAAGIDLGSILNDITATLPCFRFSELLKRAYSFC
                     KEVQSLGRELLTALEKNDSEGLALLRSSREIVLQNKILAIRNRQVDEANQLIDALNQI
                     LAEVRIRQQYYSTRPYMNAGEIIATALAGTAIISYTLGSSSDIIAAIAHLIPAAFLGA
                     MGIGGSPAASVTYGGDNIGKSTGKASDGFKALGKILEIATARADAQGRYQRSKDEWDF
                     RNNEATQEIAHVQSQILAATIHLDVVQKQVEQQNIVVDNTQAVDDFLHNKFTNQDLYS
                     WMMGQISTVYFQAYQLAYAMAKSAEQCFHFEQGPSTTTYIQFGYWDNLHKGLLAGDLL
                     YYDLERMESDYLAQHCRPLEITKIISLAQIAPDRLMLLKETGECDFDLPELLFDLDYP
                     GHYKRRIKLVRITVNCPSTSGAPCSPNVSSTLTMTKNSERISTDLSAGYTRTGLSDTR
                     FIDNVAPVQSIVTSNAVFDSGMFSDTELFKLEVRDGRYMPFEGAGAISSWHLELQQDT
                     NQFDTSSITDVTLTFLYSARDGGSALKTAAKQAVTDALPKNGILLIDAAAKFPTQWQG
                     FLHPSGGTDQILNLAITPQLFPFYARNRTITITQVDLLAQFTPSTTTLIAEFVPPMAS
                     SDPHPSLATGSPYDPLLHASITTLSVVIDNSTPQWQLRLQISGAADFKSLPPTQLADV
                     YLLLYFTVS"
     gene            5069..5383
                     /locus_tag="VGT44_01750"
     CDS             5069..5383
                     /locus_tag="VGT44_01750"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEV2579548.1"
                     /translation="MIDPAIHEMVRKIVLTYKPDRVLLFGSYAKGTQTPFSDIDLLVV
                     KDTAQPRHRRGHELRQLFYGNLVSVDLLVYTNQELEDEMMEPHSFLSSIAQSWIEMPM
                     SE"
     gene            complement(5503..6045)
                     /locus_tag="VGT44_01755"
     CDS             complement(5503..6045)
                     /locus_tag="VGT44_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579549.1"
                     /translation="MSEAPAKKRPLLAYRVRVVLFLIGIAIIVVLTLLYAPRERDAAN
                     VSGDAIPITVTVTNPLGSLTVNRGVNFQSAFITVTQVMQASSFSDDTKRGGNYVVRVE
                     LSAQNKSNPQSPINIDYPSQARLLLANGQIIIPRLISLSPILLPHATVSGFIDFALSS
                     PGQLSSMTLVLGGNTKVAFS"
     gene            complement(6131..6499)
                     /locus_tag="VGT44_01760"
     CDS             complement(6131..6499)
                     /locus_tag="VGT44_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579550.1"
                     /translation="MQEPQESQQWQTGLEYGEYRANAGQFDSAQQQKIYPQEERGLMG
                     TAAAIVTIVLSSIGFGAAIVGIVGASIVLYYSGGQHYMLVGGIMGLVSSILLLILMIA
                     LFVVNVVTLARWQMRSRPRA"
     gene            6750..7484
                     /locus_tag="VGT44_01765"
     CDS             6750..7484
                     /locus_tag="VGT44_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2579551.1"
                     /translation="MNTSPLLEREEQQQTTEQHIVMDVRDITKSLPLGRERIEILKGI
                     SFQIMGGEFVSIVGPSGSGKSTLLGIIAGLDNPTTGQVLIDGVDITRMGEGKLAAVRN
                     SKIGMVFQAFNLIPTLTAQENVEVPLYVGKHKGSPSARAKELLDLVGLSHRLDHRPNQ
                     LSGGEQQRVAIARALATDPALVIADEPTGNLDARNGENVLKLIATLREQTGKTFIIAT
                     HDPVVASRADRAIRIVDGVIAAIDND"
     gene            7536..>8034
                     /locus_tag="VGT44_01770"
     CDS             7536..>8034
                     /locus_tag="VGT44_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579552.1"
                     /translation="MKASMYFNYTSRSLLRGGQRTILAVFCVAVGVMAIVALQLVGNM
                     LQGSLTTNTRDTNGGDIAVTAQGVPFTSSDLGYFNQLKSDKTITNYTAVINSTGSLNA
                     AASSFSSFTVEAVDPSTFPVVSQPTFASPANGSVSNLLANNQVIVTQSFLDNSGKKMG
                     DSFDVY"
BASE COUNT         1923 a         2456 c         2023 g         1632 t
ORIGIN      
        1 gaccatcaac ctgctccata agattgcact gttgctgcaa aggctcgcca ttacgagcag
       61 tgatgatcta agtaagttgc tcgcgaatcc agcctactac ggcggcctcg acctgtcgca
      121 attgcccgta cagaccgcgc agcccgcgct gtcgttcagc gcgtggctgc aactggcgca
      181 gattatgcag ttcaaagcgc agtatccagc gccacagggc gtgaatttcc tcgatatgct
      241 cgcagcggcg cagaccgcac ccaaccagtc cggtattctg acatcactca gcacccttac
      301 cacctgggac ctcactgacc tccagacagt ggacagcgca ttggctctca cagtcgccga
      361 ttaccagaag attgccacat accagcgact cgcggcctgc ttcgcgtcga tacagaggct
      421 gggcgtcaag gcacaggcag ttatcgcctg ggcgcagcag ccgtcctcgc aggacatttc
      481 cacgcagatc aagcaggcca cgaaggcgaa atacccaaac gaccagtggg tcacgatcgc
      541 agcgccgctt caggacgcca ttcgcgacct caaacgcgcc gcgctggtca attacctgat
      601 cgctcatccc gacccgccga atgggaagaa ttgggcagac gccaacgatc tcttctcgta
      661 cttcctcatc gatgtcgaga tgacctcttg ccagcttagc tcgcgtatcg tgcaggcgac
      721 aaacgcgatc cagctctttg cgcagcgctg cttgatgaat ctcgaaccgg acatcactgc
      781 cgatgtccag aatgaccctg attggcagca atggcagtgg atgaaagcct atcgcgtatg
      841 ggaggcaaat cgcgaggtct tcctgtaccc cgaaaactgg attctgcccg aattacgcaa
      901 gaataagtcc tccttcttca aggacttgga aagcgacctg ctgcaaaacc agatcacaaa
      961 cgacaacgtt gaaacaggga tgctctctta cctgcaaaag ctcaacgatg tctcgcggct
     1021 cgaagtgtct gccatgtatc acgatgacga actaggtatc ctgcacgtct tcgcgcgaac
     1081 gcacgatgtt cctcccattt actactatcg ctatctcaaa gatggggcgt ggagcggctg
     1141 ggagaagatc aacctggata tcaagagcga tcatctcatt ccggtagtct ataaccgcaa
     1201 gctctacctc ttctgggcgt tgtttacccc aaagtcggag aagcacgaaa atcaacctat
     1261 tgcccccgcg cagggaggtc agacatacaa cacaccgccg ccacccaacc agtattggga
     1321 gattcaactg gcctggagct accttcacga tggcaaatgg atagcgcagt cgcactcgaa
     1381 tatgattctg attcatcccc aacgcgttgt gggaagccag cctgtttcgc cgctgcgtcc
     1441 ccagttcgcc tacaccttca aggccatgat gcgcggcgac gacctggtga tcaacgcgtt
     1501 catcagcccg accatcgaat ttaataatcc catcggaaac tacaacgaga ccgggcttcc
     1561 ctggcactcc tcggagttcg tcttcaacag caccgtcact gctatcaatg ttgtcgatat
     1621 caacggattc cagcagtgga tatacaacaa ctatagtgct gacgggcgtg ctatcggaac
     1681 agtgcagcaa cagcaaagca gcttgcttct gccactgcta tcacactaca acaacgaaga
     1741 cctggtgaat atcagtacaa acggcggcca gttgtacgtc ctcaagcaaa gcagtctgcg
     1801 cctggataat ggacagttgc tcgcagccgc gggtcttcct tacgaggtgg ttgtgccaca
     1861 ccaggacgca caattcgact cgacccggcc tttcttctat caggataagc agcgagccta
     1921 ctttatcact ccgaccatcc tctaccagaa tggcagcacc ttcacgccga atctgccggc
     1981 cagcccgtac aacgcgcagt acgaggtcgc gtaccagttc gatgagtttt atcacccctt
     2041 caccaaactc ttcatccacg aactgaacaa gctgggcgtt cccggtctgt tcgaccgccg
     2101 cctgcaaacc aatcccggcg ccgcgccgtt ccccattgat agcttcagct tctcgtctgt
     2161 ctaccagcca acatcgctgg tgacggcgga caacgaccag gagatcgtcg atttctcgcc
     2221 gggtggggcc tactcgctct ataactggga actgttcttc catgccccgc tcatgctggc
     2281 cctgcgcctg aaccagaacc agcgttttga agaggcggcg aagtggttcc actacatttt
     2341 cgatcccggc agcgcgtcga gcgatctgcc ccctcaacgg tactggatca cgaagccatt
     2401 tcaattgcag acagccgaca tcctgaatca gcgtatcgac aatctgctca agctgatcaa
     2461 tcagcacgat cccacgtatg aggcgcaggt aacccaatgg cgcaacgatc ccttcgatcc
     2521 gcacatgatt gccaacctgc ggcccgtggc gtatatgaag acgacagtga tgaaatacct
     2581 ggacatgctc atcgcctggg gcgacaacct gttcagccag gacaccatcg agacgatcaa
     2641 ccaggccacc cagctttata tcctggcggc gcaaatcctg gggccgcgcc cccagcaatt
     2701 gccagccttg cagcgtgccg ccgacaaatc cttcaacgac cttgaaaaag acctcgacgc
     2761 tttcggcaac gccatgacag aagtggaaaa cacggtcgtc atcagcggtg acggcagcag
     2821 ccccaatccc aacacgcccc agttgcccca gttgcagtcg ttctatttct gcattcccgc
     2881 caactcccaa ctgctcggct actgggatac ggtcgcggat cgcctcttca agattcgcca
     2941 ctgccagaac atccagggcg tgacgcagca gctcccattg ttctcaccgc cgatcaatcc
     3001 agctctgctc gtacaggctg ccgccgcggg cattgacctg ggcagcatcc tcaacgacat
     3061 cacagctact ctcccctgct tccgtttcag cgagctgctg aaacgagcct acagcttctg
     3121 caaagaagtg caatcgctgg gcagagagct gctcaccgca ttggaaaaga acgattcaga
     3181 aggattggcc ctgttgcgtt catcgcgaga gatcgttctc caaaacaaaa tcctggcaat
     3241 tcgcaatcgg caggtggacg aggccaatca gttgatcgac gctctgaacc agatattggc
     3301 tgaggttcgg atccgccagc agtattacag cactcgtcca tacatgaacg cgggagaaat
     3361 aattgctact gcattagcag gtactgccat aatttcgtat acgctcggaa gcagcagcga
     3421 catcattgct gcgattgcgc acctcatccc agctgcattc ttaggcgcaa tggggatcgg
     3481 aggatctccc gctgcttctg taacgtatgg tggtgataat ataggcaaat ctaccggaaa
     3541 ggcttccgat ggatttaagg cgctaggcaa aattctggag atcgcgacag ccagagccga
     3601 cgctcagggg agataccaga ggagcaaaga tgaatgggac ttccgcaaca acgaggctac
     3661 tcaagagatc gcgcacgtcc agagccagat tctggccgcc actatccatc tcgatgtcgt
     3721 ccagaagcag gttgagcaac aaaatatcgt tgtggacaac acccaggccg ttgacgactt
     3781 cctgcacaat aagtttacga atcaggacct ctatagctgg atgatggggc agatctcgac
     3841 cgtctacttc caggcctacc agctggccta cgccatggcg aagagcgccg aacaatgctt
     3901 ccactttgaa cagggacctt cgactacgac ctacatccag ttcggctatt gggacaacct
     3961 gcataaagga ctgctggcgg gcgacttgct ctactacgat ttagagcgga tggagtcgga
     4021 ctacctggcg cagcactgcc gaccgctaga gatcacaaag atcatctcac tggcccagat
     4081 tgcgcctgac aggctgatgc tgttgaaaga gaccggcgaa tgcgattttg acctgccgga
     4141 actactcttc gacctggact atcccggcca ctacaaacgg cgcatcaaac tggtgcgtat
     4201 caccgtcaac tgcccctcaa cgtccggcgc accttgtagt cccaatgtga gcagcacgct
     4261 gacgatgacg aagaacagcg agcgcatcag tacagacttg agcgccgggt atacacgcac
     4321 tggtctatcc gacacgcgct ttatagataa cgtggctccc gtgcagtcga ttgtcaccag
     4381 caacgccgtg ttcgatagcg gtatgttcag cgacaccgag ctattcaagc tcgaggtgcg
     4441 tgatggacgc tatatgccct ttgagggcgc gggcgcgatc agttcctggc atctcgaatt
     4501 gcagcaggac accaaccagt tcgataccag cagcattacc gatgtgacat tgactttcct
     4561 atacagcgcc agagatggcg gctcggctct caagacggcg gcgaagcaag ccgtgaccga
     4621 tgctctgccc aaaaatggca ttctgctgat cgacgctgca gcgaagttcc cgacgcagtg
     4681 gcagggcttc ttgcacccga gcggtggcac cgatcaaata ctcaacctgg cgatcacgcc
     4741 acagctgttc ccgttctacg cacgcaatcg cactatcacc atcacccagg tcgatctgct
     4801 ggcgcaattc acgccgtcca cgacgacact catcgccgag tttgtcccgc cgatggccag
     4861 tagtgacccg cacccatcgt tggccactgg cagcccgtat gacccgctgc tccacgcatc
     4921 cataactaca ttgagtgtcg ttatcgacaa ctcaacgccg cagtggcaac tgcgcctgca
     4981 aatctccggg gcagccgatt tcaagtcgct gccgccgact caactggccg atgtgtatct
     5041 gttactctac ttcacggtga gttaaacaat gattgatcca gctattcatg agatggtacg
     5101 caagatcgta ctgacgtaca aacccgaccg cgtactgctg tttggctcct atgccaaagg
     5161 cacgcagacg ccattcagcg acatcgacct gctcgtcgtc aaagatacgg cgcagcctcg
     5221 ccaccgtcgc gggcatgagc tgcgccagtt gttctatggc aacctggtga gcgttgatct
     5281 cctggtctac accaaccagg agctagaaga cgagatgatg gagccgcatt cgtttctctc
     5341 ctcgattgcg caatcgtgga tagagatgcc tatgagtgag tgatatcaaa tccggttgat
     5401 tactgtacat actatagcgg caggactggc cccaccattg tcggccacaa tccaaatgaa
     5461 cacgatctaa acggatttga tatgatgggt acctgacccc atctacgaaa acgccacctt
     5521 cgtattgccg ccaagaacga gcgtcatgct cgatagctgc cccggcgacg aaagcgcgaa
     5581 atcaatgaag ccgctgaccg tcgcatgagg cagcaagatg ggggagagcg aaatcagccg
     5641 gggtataatg atctgcccat tcgccaggag caaacgcgcc tgggagggat aatcgatgtt
     5701 gatggggctt tgcgggttgc tcttattctg cgccgacaac tccacgcgca caacgtagtt
     5761 acccccacgc ttcgtatcgt cagagaatga ggacgcctgc atgacctgcg tcaccgttat
     5821 aaacgcgctc tgaaaattta ctccccggtt gaccgtcagc gagccgagcg gattggtaac
     5881 agtcacggta atgggaatag catcgccgct gacattggcc gcgtcacgct cacgtggcgc
     5941 gtaaagcaag gtcagcacga cgataatagc aatgccgatc aaaaacagga ccactctgac
     6001 ccggtatgca agcaatggac gtttcttcgc tggcgcttct gacatggatt tgtacttcta
     6061 cttctacacg ttgatttgtt gccagcatta ttatagcgaa ggttgtccag aggtgacaat
     6121 gccataccgg tcaagcgcgg ggcctgctac gcatctgcca gcgagccagc gtgaccacat
     6181 tcaccacaaa aagtgcaatc atcaggatca acaagagaat agaactgacc agtcccatga
     6241 tgcctcccac cagcatataa tgctgcccgc ctgagtaata caggacaatc gatgctccca
     6301 caatgcctac aatcgcggcg ccaaatccaa tgctagaaag gacgatggtg acaatcgcgg
     6361 ctgctgttcc catcagcccg cgttcttctt gtggataaat cttttgctgc tgcgctgaat
     6421 cgaattgccc cgcgttcgcg cgatattcgc catattcaag accggtttgc cattgttgtg
     6481 attcctgtgg ctcctgcatt cgcattgcat tttccctctc atgacaccca ggaaagcttc
     6541 accgcatttc ttctcataca tacgatacca cgttgctatg gttgtacgca cgatctccgc
     6601 caatccaact tatctctaca gtcctacagt ccctcccaca acaggtctcc agtcgcatct
     6661 caaatcatag cccctcgcgt atgctagaac catgttacgg gtgtgacaca cagcactaaa
     6721 tcgagcaaaa caaaaggagc aacaatccaa tgaatacaag tccacttctc gaacgtgaag
     6781 agcaacaaca aacgacggaa cagcacatcg tcatggacgt gcgcgacatt acgaaaagcc
     6841 tgcccctggg acgcgagcgc atcgagattc tcaagggcat cagcttccag atcatgggcg
     6901 gcgaattcgt ctccatcgtc gggccgtccg gttcgggcaa aagcacgctg ctgggcatca
     6961 tcgccggcct ggacaatccc accaccgggc aggtgctgat cgacggcgtc gatatcacac
     7021 gcatgggcga gggcaagctc gcggcagtgc gtaacagcaa aatcggcatg gtcttccagg
     7081 cattcaacct gatccccacg ctgacggcgc aggaaaatgt cgaggtgccg ctctacgtcg
     7141 gcaagcacaa aggttcgccc tcggcgcgcg cgaaggaact cctcgacctg gtcggcctct
     7201 cacaccgcct cgatcaccgt cccaaccagc tctccggcgg cgagcagcag cgcgtcgcga
     7261 tagcccgcgc cctggcgacc gatcccgcgc tggtcatcgc cgacgaaccc accggcaacc
     7321 tggatgcgcg caatggcgaa aacgtcctga agctgatcgc cacgctgcgc gaacagaccg
     7381 gcaaaacctt catcatcgcc acgcacgacc ccgtggtcgc ctcgcgcgcc gaccgcgcta
     7441 tccgtatcgt cgatggcgtc atcgccgcca tcgacaacga ctaaacccag ggcgaccgcg
     7501 agggtcgccc tggatatcca gaaaggatta cgatcatgaa agcttcgatg tacttcaatt
     7561 acacgtcgcg gtcgctgctg cggggcgggc agcgcaccat cctggcggtg ttctgtgtgg
     7621 cggtcggcgt gatggcgatt gtcgccctgc aactggtagg caacatgctg caaggctcgc
     7681 tcacgaccaa tacgcgcgat accaatggcg gcgatatcgc ggtcacggcg cagggcgtac
     7741 cgttcacctc gagcgacctg ggctacttca accagctcaa gagcgacaaa acgatcacga
     7801 actacacagc agtcatcaac tcgaccggtt cgttgaacgc ggcagcatcc tcgttttcat
     7861 cctttacggt cgaggcggtt gatccgagca ccttcccggt tgtctcccag ccaaccttcg
     7921 cttcaccggc gaatggcagc gtttcaaacc tgctcgccaa taaccaggtg atcgtcacac
     7981 agagctttct cgacaactcc gggaagaaaa tgggagatag cttcgatgtc taca
//
LOCUS       DASZAK010000107         4602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_251093, whole genome shotgun sequence.
ACCESSION   DASZAK010000107 DASZAK010000000
VERSION     DASZAK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251093"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1623)
                     /locus_tag="VGT44_01775"
     CDS             complement(16..1623)
                     /locus_tag="VGT44_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579553.1"
                     /translation="MATFVKAPCDEGVIRSAPDTAPSPPRVGLWVLVATILGSSMALI
                     DGSVVNVALPIIQRDFNTSATNVQWVVEAYALFLAALILVGGTLGDHFGRRRIFAIGM
                     IVFTLASIACGFSPNVLFLILARAVEGFGGALMVPGSLAIISASFSQDQRGRAIGTWS
                     GFTSIASLPGPVLGGWLVQYASWRWVFFINVPLAAIALIVLFWRVPESRDEDASGRLD
                     WWGILLETLALGALVFGLIQAGSLGLGSPIVLSMLAIGIAALIAFLLVEARSSAPMVP
                     LTLFRSPTFSGANLLTFLLYGALSGITYYLPFNFIQVQGYSPTFAGAAFVPFSLIMFL
                     LSRWAGGLVNRFGAKLPLVVGPVITALGFALFAVPDITSGPGSFWTTFFPAVVVMGLG
                     MTITVAPLTTAVMGAVEQRHAGIASGINNAVSRIAGLLAIAIFGIVVLSVFTSSLDSH
                     LATLHLSPGVKYAIDAQSVKLAGITIPTNVGSETQIALKKAIDESFVNSFRLVSLICT
                     ILALASALSAWLLVAGKKPAPVGSAVK"
     gene            complement(1658..1867)
                     /locus_tag="VGT44_01780"
     CDS             complement(1658..1867)
                     /locus_tag="VGT44_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579554.1"
                     /translation="MNVIKAIYNFIVGDMIILIGVALALAVLAIINNVPALAPIRGLS
                     GPILIIAVLASLVATLSREAFSPTR"
     gene            complement(1864..2685)
                     /locus_tag="VGT44_01785"
     CDS             complement(1864..2685)
                     /locus_tag="VGT44_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579555.1"
                     /translation="MQWVVATAAFLASAVEFVEAFTIVLVVGVTVNWRSALLGALAAA
                     ATLAILVATLGTALVQWVPLDVLRTVIGTLLLLFGLKWLKNAIMRYAGLKARHDEQAI
                     YEETRAELRARDEVDTSSSRFDLFGFLLSYKSVLLEGLEVAFIVITFGLSAASSTVSR
                     SSGIASAALGALAAGLLVILVGAIVRVPLANVPENTLKFVVGVMLTTFGTFWLGEGFG
                     VEWPLSDAFLLILIAIYLSASFLLVVWLKQVRKQRAQAIGDQSAPTTVQTQEVNP"
     gene            complement(2871..3596)
                     /locus_tag="VGT44_01790"
     CDS             complement(2871..3596)
                     /locus_tag="VGT44_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEV2579556.1"
                     /translation="MSAILLSLATFFSTLIGGLFGLRHRDHLNLILGFTAGVLVGLVS
                     FDLLPEIFRLVYTLHLDSTGPMLALIGGFLLFHIVEKSILIHFSHEELYAVHQHPLVG
                     ILSAIALTGHSFLDGVGIGLGFQVSAAVGLIVAIAVISHDFSDGLNTVTLMIVNKNTP
                     RRSFLLLLADAVAPVAGAISTMFFTIPAVGLPYYLGFFAGFLLYIGASDILPQAHSNK
                     SSALTLVLTVLGVVFAFVVTRFV"
     gene            complement(3688..4347)
                     /locus_tag="VGT44_01795"
     CDS             complement(3688..4347)
                     /locus_tag="VGT44_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579557.1"
                     /translation="MRKQMHQTTSNDAEEVVVPSVTALIGILVTGLLYLVLPANLVIG
                     PNWLLLVVEVVFVVPLVIDVLTAWDLSHTARRMLVLVPLGVSTLALIVGVALLIYSLP
                     TDTHATNLLRTAALLWSFNILVFALWYWEVDGGGPWKRHLAGHRAADFLFPQQTTGEH
                     WAPHFLDYLFVAFTGATALSPADTYPLTRLAKMLMMCEALLSLTIAAILIARAVNILG
                     G"
BASE COUNT         1231 a         1263 c         1251 g          857 t
ORIGIN      
        1 atataaccag tatacttatt tcactgcgct tcccacaggg gctggtttct ttcctgcaac
       61 caacaaccag gcgctgagcg cacttgcaag tgccaggatg gtgcagatca gcgagaccag
      121 gcgaaaactg ttcacgaacg attcatcgat cgctttcttt agtgctatct gtgtctcact
      181 acccacattc gtagggatag tgattccagc cagtttgacg ctctgcgcat cgatagcata
      241 tttcactccg ggcgagagat gcagagtcgc caggtggcta tcgaggctgc ttgtgaagac
      301 agataagacg acaataccga atattgcgat ggccagcagc ccggcaatgc gagacacagc
      361 attattgatc cccgaagcga tacccgcgtg gcgttgctct accgcgccca tgaccgctgt
      421 tgtgagcggt gcgactgtga tggtcatgcc gaggcccatc accaccaccg ccgggaaaaa
      481 ggtggtccag aaactgcctg ggccactcgt gatgtctggc acagcaaaga gagcaaagcc
      541 gagcgcggtg atgaccgggc caaccaccag cggtagcttc gcgccaaaac gattcaccag
      601 cccaccggcc caacgcgaaa gcagaaacat gatcaggctg aacggcacaa aggcagcgcc
      661 agcaaaggta ggagaatacc cctgaacctg aatgaaattg aagggtaagt agtaggtaat
      721 gccgctcagt gccccataca gcaagaaggt gagaagattt gcaccgctaa acgtaggcga
      781 acggaacaag gtgagaggga ccatgggtgc cgagctacga gcttctacca gcaaaaatgc
      841 gatgagtgcg gcaatgccaa tagcaagcat gctgagcaca atgggactgc ccaggccaag
      901 ggaacccgcc tggatcagcc cgaagacgag cgctcctaac gcaagcgttt ccagcaggat
      961 gccccaccag tccagccttc ctgacgcatc ttcatcacgg ctctccggta ctcgccagaa
     1021 gagcacgatg agtgcaatgg ctgccagggg aacgttaata aagaagaccc agcgccagga
     1081 ggcatactgt actaaccacc ccccgagcac tggacccggc aacgaggcga tcgaagtaaa
     1141 ccccgaccag gtgccaatcg ctcgaccgcg ctggtcttga ctaaacgagg cactgataat
     1201 cgccaggcta cctggtacca tcagtgcgcc accaaagccc tcgacagctc gtgcaagaat
     1261 caggaacagc acgttgggtg aaaagccaca ggcgattgag gcaagggtga agacgatcat
     1321 gccaatggca aagatgcgcc gtcgcccgaa gtgatcaccg agcgtccctc ccacaaggat
     1381 gagggcagcc agaaaaagcg cataggcctc cacaacccac tggacattgg tggccgatgt
     1441 gttgaagtcg cgctggatga tcggaagcgc gacattgacg actgaaccat caataagggc
     1501 catgctcgat ccaaggatgg ttgcgaccag gacccacagc ccaacacgtg gagggctggg
     1561 cgccgtgtca ggagctgagc gaatgacacc ctcgtcacag ggtgctttca caaaggtagc
     1621 catctttggt tctccttccc tttatttgta cattcagcta gcgcgtgggg gaaaacgcct
     1681 cccggctcag cgtggcgacg aggctggcga gcacagctat gatcaggatt gggcccgata
     1741 atccccttat gggggccaga gcagggacat tgttgatgat ggccaggaca gccagcgcca
     1801 gcgcaacacc tatcagtatg atcatatcac caacgatgaa attgtaaatg gctttgatga
     1861 cgttcacgga ttcacctcct gagtttgtac agtagtggga gctgattgat cccctatggc
     1921 ctgcgcccgc tgctttctca cctgtttgag ccagacgacg agcaagaaag atgcgctcag
     1981 gtaaatggcg atgaggatga ggagaaaggc atccgacagc ggccactcca ccccaaatcc
     2041 ctcccccaac cagaaggtgc caaaggtcgt caacatgacc ccgacgacga acttcagcgt
     2101 gttttcgggc acgtttgcca atggtacgcg cacgatcgcg cctaccagta tgaccagcag
     2161 gccagcagcg agcgcgccaa gcgcggcgga agcaataccg ctggagcgcg agacggtgct
     2221 tgaggccgcg ctcagcccaa aggtgatcac aatgaacgcc acttcgagtc cttcgagcaa
     2281 gaccgacttg tacgatagca gaaagccgaa gaggtcgaaa cgggatgaag aggtatcaac
     2341 ctcatcgcga gcacggagtt cagcccgggt ttcctcgtaa atggcctgct catcgtggcg
     2401 tgctttcaaa ccagcatagc gcatgatggc gttcttcaac cacttcaaac caaagaggag
     2461 gagaagcgta ccgatgaccg tgcgaagcac atcaagagga acccactgga caagtgcggt
     2521 acccagcgtc gccaccagaa tggcgagtgt cgccgcggcg gctaaagccc caagcagcgc
     2581 gcttcgccag ttaaccgtta caccgacgac aaggacgata gtaaacgcct ccacaaactc
     2641 tacagcggag gccaaaaacg cggctgtagc aacaacccat tgcatagaga ggctcctttg
     2701 tttagctgct ttcatcgatc gcagcttctt cttgtccctg ctccgttgga gcagggatgg
     2761 tactagatag cctaaacctt tacatagcgt acacgcacgg cgtcaagaaa agcgttggcc
     2821 cgcgcgtaaa agcggatgtc gagaatttat cgttgcagcc tcctcagcgc ttagacaaag
     2881 cgcgtaacga caaaggcgaa aacgactccc agcactgtga gaaccagggt gagagctgaa
     2941 ctcttgttgc tgtgtgcttg cggcaagata tcgctcgccc caatgtagag caggaagcca
     3001 gcaaagaagc ccaggtagta cggcaatcca actgcgggaa tcgtgaagaa catggtggag
     3061 atggcaccgg ccaccggtgc taccgcgtcg gccagcaaca acaggaagga acgacgaggc
     3121 gtgttcttat tgacgatcat caacgtgacg gtattgaggc catcggagaa gtcgtgagat
     3181 atgacggcaa tggcaacgat cagtccaacg gcagcactca cctgaaagcc aaggccaatg
     3241 ccgacgccat ccagaaagct atgaccagtc agcgcaatcg ccgagaggat accaactagc
     3301 ggatgctgat gcacagcata cagctcttca tgagagaagt gaatgagtat tgacttctcg
     3361 acgatatgaa acaggagaaa gccgcctatc agtgcaagca tgggtcccgt actgtccagg
     3421 tgcagcgtgt agacgagccg aaaaatctcc ggcaacaggt caaaggacac caggccaacc
     3481 agcacacctg ccgtaaaacc caggatcaga ttgagatgat cgcgatggcg cagcccgaaa
     3541 agaccgccta tgagcgtcga gaagaacgtc gcgagcgaca gaagaatagc gctcattcct
     3601 tgcttttcat ccttccactt taatagatca ggtcaacgaa gacacctaca tcaacttaac
     3661 agtgataccc agtgatagac gaccggttca ccctccgagt atattgactg cgcgggcgat
     3721 cagtatcgcg gcgattgtga gtgaaagcaa cgcttcgcac atcatgagca tcttcgccag
     3781 tctcgtcagc ggatacgtat cagcgggact cagcgcggtt gcaccggtaa aggcaacgaa
     3841 gagataatca agaaagtgcg gcgcccaatg ctctccagtt gtttgttgtg gaaagaggaa
     3901 atctgcggcg cgatgcccgg cgagatggcg cttccatggc cctccaccgt caacttccca
     3961 ataccacaag gcgaagacca ggatgttgaa agaccacagc agggccgcag ttcgtaataa
     4021 atttgtcgcg tgtgtatcgg ttggcaacga gtaaatgagg agcgcaaccc caacaatgag
     4081 cgccaatgtg ctcacaccga gaggtaccag cacgagcata cgcctcgccg tatgtgagag
     4141 gtcccatgct gtcaagacat cgatgacaag agggaccaca aacaccactt ccacgaccaa
     4201 caggagccag tttgggccga tgaccagatt tgctggaaga accagataga gcaggccggt
     4261 caccagaatt ccgatgaggg cagtcacgct tggaacgacc acttcctcag cgtcatttga
     4321 agtcgtttga tgcatctgtt ttctcaagtg tactaatcat tatgccacaa aagatcctgt
     4381 ttcctagcgg gcgctttgtt tcctttcttg catatatggc atgctcttca ttctttccat
     4441 cgcccgattg caaacagagg ggcatttctg cttgtcatta gtatgataga tgttgaaagt
     4501 gaaaaaaacc gtgtgtcaga ccgtgaaaat gatggagcat cgagagtctg atcaggctgc
     4561 ctttgacatc tgtttcacat catcgccttt gtgacccctg ag
//
LOCUS       DASZAK010000108         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_37913, whole genome shotgun sequence.
ACCESSION   DASZAK010000108 DASZAK010000000
VERSION     DASZAK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37913"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VGT44_01800"
     CDS             complement(<1..736)
                     /locus_tag="VGT44_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HEV2579558.1"
                     /translation="MMNVHLEQGESLHSEDAPPIGRTEAELTRTYGDHTLAFFGLAPE
                     NEHFLAPGGRGLVNYRLMNNVAVVAGDPLCAPEEVEQVTRSFLRFCASHHWSVAFYQA
                     GPDNLAIYRVLKMRPMKMGEEAILAPQTFTVQGAALANVRTSCRRAEREGIRVEWYEG
                     VPPAAVMQQLEGVSSAWLESKAGKQTEESGFSTGRLGEVEISALRADRVAEMTMPSHP
                     ALPAPQLVTGVAFTSSSEACAFVTFTP"
     gene            complement(778..2871)
                     /locus_tag="VGT44_01805"
     CDS             complement(778..2871)
                     /locus_tag="VGT44_01805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2579559.1"
                     /translation="MDETITTGPQAEPENPPDQPGQRRFLLWHRLSLGGILLIAVFMD
                     FFQLGANGFGSYYPPAVRSMMDNWHNFFFAAYDPGAFVTIDKPPVGFWLQVASAKIFG
                     FNAVSILAPQALAGVLSVLLLYYLVRRHFGVTAGLLAALALALSPISVVTNRNITIDS
                     TLALTLLVGAWAVLRAAETGKLRWLLLSALIVGIGFNIKMLEAYLVVPAYAVLYLVTA
                     PRSIWVRIGHLSLAGLLLVVVSLSWVVAVDLTPTSQRPYVDSTQDNSEISLAFGYNGL
                     ERLLGMGGPSGTKGGPTTIQGNATQQPQPSGGTPVGIFVTSAIGPLRLFEVQIGSQAG
                     WLLPLALLGMLALAWQPRQNVREDRKFQSLVFWGTWLLTMGIYFSVASGILHQWYLTV
                     MAPAIAAFFGIGLVTMWQDYRRGGWRGWLLPVALAATAAEQIYLLSDYPTWGRWMIPL
                     IAALGIVVIVALVGTRISRQLRDKAPNIRFLLPALGIGVVALLFAPTVWAVTPIFLST
                     QADTLLAGPPQTGDNGGGGFVISTGSQTFTGTLPGKVGGNTATNNDALIRYLEANQGN
                     AKFLVAVPNSQGIGDQIILATNKPVMSLGGFSNDPILTTGQLAALVKNGTVRFFLLQS
                     VGLPQQILNQIPEQYRDQLQGGPGPNSALSTWVTQHCKTVPASLWQSTSTGSTSGGGS
                     GNGNGNQLYDCATTH"
BASE COUNT          648 a          882 c          835 g          593 t
ORIGIN      
        1 taggggtaaa ggtcacgaac gcacaagctt cgctggaaga tgtaaacgcc actccggtca
       61 ccagttgcgg cgcgggcagc gctgggtgag atggcatcgt catttcagcg acccggtcgg
      121 ctcgtagtgc gcttatctcc acctcgccca atctccctgt gctgaaacca ctctcctccg
      181 tctgcttgcc tgctttgctc tccaaccatg cgctagagac gccttcgagt tgctgcatca
      241 ctgccgcggg cggaacacct tcgtaccact cgacgcgtat tccttcacgc tctgcgcgcc
      301 ggcaactggt gcgtacattc gccagagccg cgccttgcac ggtaaaggtc tgaggagcaa
      361 ggatcgcctc ttcacccatt ttcatgggac gcattttgag gacgcgatag atggccaggt
      421 tgtcgggacc ggcctgatag aaagcaacgc tccagtggtg cgaggcacag aaccgcagga
      481 agctgcgcgt aacctgttcg acttcttcgg gcgcgcacag gggatcaccg gctactactg
      541 ctacattgtt catcagccga tagttcacta accctcttcc tcccggcgcc agaaaatgct
      601 cattctctgg ggccaggccg aagaatgcca gcgtatgatc tccgtaggtt cgtgtcagtt
      661 ccgcttcggt tcgaccaatc gggggagcgt cttccgagtg aaggctttct ccctgttcca
      721 ggtgtacatt catcatttac acaatctatt gaacgagcgc tctgacctcg caggtcgtca
      781 atgggtcgtg gcgcaatcat acaactggtt tccattccca ttaccggagc caccgccaga
      841 agtggaaccg gtcgaagtag attgccacag acttgccggt acggtcttac agtgctgcgt
      901 cacccaggta ctgagcgcgc tattgggtcc ggggccaccc tgtagctgat ccctgtactg
      961 ctcagggatt tgattgagaa tctgctgtgg cagccccacc gactgtagca ggaagaagcg
     1021 caccgtgcca tttttgacca gcgccgcgag ctggccggtt gtcaggatgg gatcgttaga
     1081 gaagcctccc agcgacatta ccggcttatt cgtcgcaaga atgatctggt ctcctattcc
     1141 ctgtgaattc ggcacggcaa ccaggaattt cgcgttgccc tgattggctt caaggtagcg
     1201 gatcaacgca tcattattgg tggcagtgtt gccaccaacc ttaccaggga gagttcctgt
     1261 gaaggtttgc gagcctgtgc taattacgaa accgccgccg ccattgtctc cagtctgcgg
     1321 cgggcccgcc agcagcgtat ccgcttgcgt actcaggaaa atgggagtga ccgcccatac
     1381 tgttggagca aacagcaggg ctaccactcc gatcccaagc gccgggagga ggaagcgaat
     1441 attgggcgct ttgtcgcgca gttgccggga gatgcgggta cccaccagcg ccactatcac
     1501 aacgatgccc agggcggcga tgagcggaat catccagcgg ccccatgtag gataatcgct
     1561 gagcaggtaa atctgctcag cagcggtggc agccagcgct acaggtaaga gccagccgcg
     1621 ccaaccacca cgacgatagt cctgccacat cgtcaccagc ccaatgccga agaatgccgc
     1681 aatggccggt gccatcaccg tcagatacca ctggtgcaag atgccgcttg ctacgctgaa
     1741 gtagatgccc atagtgagca gccacgtacc ccagaacacc agcgattgaa attttcgatc
     1801 ctcccgcacg ttctggcgtg gctgccaggc cagcgccagc atccccagga gcgccagggg
     1861 gaggagccag ccagcctggc ttccgatttg tacctcgaag agacgcagcg ggccaatagc
     1921 gctggtaacg aaaatcccga ccggcgttcc acctgacggt tgtggctgtt gcgttgcatt
     1981 cccctggata gtggtcggtc cgcccttcgt gcccgatgga ccgcccatgc caagcaggcg
     2041 ctcgaggcca ttgtagccga aagccaggct gatctctgaa ttgtcctgcg tggaatcgac
     2101 atagggccgt tgcgaggtag gcgtgagatc gacggccacc acccaggaga gcgaaaccac
     2161 cactagcagc agcccggcca gggacaggtg tccgatacgc acccaaatgc ttcgtggcgc
     2221 ggtaaccagg tagagcacgg cgtaggcagg taccaccagg tacgcctcca acatcttgat
     2281 attgaagcca atgccaacga tgagggcaga gagcagcagc cagcgcagct tcccggtctc
     2341 cgccgctcgc agcactgccc acgccccgac gagcagggtt aaggccaggg tgctatcaat
     2401 ggtaatattg cgattggtga cgacactgat tggagaaagc gccagggcca gagccgccag
     2461 caggcccgct gtcactccaa agtggcggcg taccaggtaa tagagcaata aaacagacag
     2521 cacgccggcg agcgcctggg gcgccagaat gctgacagca ttgaagccga agatttttgc
     2581 gctggctacc tgcaaccaga aacccaccgg gggcttgtcg atcgtcacga aggcccctgg
     2641 atcataggca gcaaaaaaga aattatgcca gttgtccatc atgcttctca cggcaggggg
     2701 ataataactg ccgaagccgt tcgcaccaag ctggaagaag tccatgaaga cggcgatgag
     2761 gagaattcct cccagcgaga gtcgatgcca gagcagaaat cgccgctgcc ccggttgatc
     2821 tggtggattc tcaggctcag cttgtgggcc tgtcgtaatt gtttcatcca tctatctctt
     2881 ctccttactt tttttctcag tcagatctaa aagataaacc gcgcgtcgcc tcactcctcc
     2941 tacctgatgg cgagtata
//
LOCUS       DASZAK010000109         6016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_641756, whole genome shotgun sequence.
ACCESSION   DASZAK010000109 DASZAK010000000
VERSION     DASZAK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641756"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VGT44_01810"
     CDS             complement(<1..257)
                     /locus_tag="VGT44_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579560.1"
                     /translation="MYDSRPGTLPPCELSSHCSRLLDDLADLASTRSEDVFEQTYVPR
                     DRQDQHLVVQMRVHLPHCPTCAAALRRALALRSEQRAVLRG"
     gene            complement(257..844)
                     /locus_tag="VGT44_01815"
     CDS             complement(257..844)
                     /locus_tag="VGT44_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2579561.1"
                     /translation="MNVLENEPQLTLAACTGSMAAFEALVVYYEPRIRRMLYSLTQDS
                     QLTQDLCQETFLAAYRALPRMNGDELRFAPWLYRIALNQVRSEWRRRKHVSVMPFATP
                     QGNDDDVFDDPGEESLISGERFEEQVVQRDLIDRVMAQLPPTSVMCILLDAEGFSYRE
                     IADTLQDTLSAVRSRLSRARQSFQRLYSQLEQEGR"
     gene            complement(1030..1443)
                     /locus_tag="VGT44_01820"
     CDS             complement(1030..1443)
                     /locus_tag="VGT44_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-ribbon domain-containing OB-fold protein"
                     /protein_id="HEV2579562.1"
                     /translation="MPDTTRARPLPTVTEVNRPFWEGCRQGKLLLQYCDACQQSQFYP
                     RLYCMRCGGQQVRWIEASGRGVVYSYTVIRQNKSPEFRDDVPYNVALIELEEGPHMMS
                     NVIDIAPDDLRVDLPVVVVFDPVSDEISLPRFRPR"
     gene            1618..2517
                     /locus_tag="VGT44_01825"
     CDS             1618..2517
                     /locus_tag="VGT44_01825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2579563.1"
                     /translation="MSTPPNPRREHPSTYVVPDRSTDENTRLQLQDQMITASMGGVLP
                     EQSDRVQFKRILDVGCGTGGWLIESAKTYPTMTELVGVDVSAKMVEYAQAQAEEQQVS
                     DRVQFRTMDALRMLEFPVGYFDLVNQRFGISYLRKWDWPKLLQEYQRVTRSGGVIRIT
                     ETDIITETSSPALLRLDLLLMEALAQAGHLFTAERDGLTSQLANLMRQHGLQNIQTRA
                     CDLVYRAGTPEGHMFHEDMKQVYRNFLPFLRKWTRVPEDYETLYQQMLSETQQADFVA
                     TWNVFTAWGNAPVKTARFAPSTI"
     gene            2574..3728
                     /locus_tag="VGT44_01830"
     CDS             2574..3728
                     /locus_tag="VGT44_01830"
                     /inference="COORDINATES: protein motif:HMM:NF004811.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HEV2579564.1"
                     /translation="MPSYKGRIVIAGVAESDYGRVPHLTELELHAQALQRVLEESGAR
                     KQDIDGVFCSSHMMGMPTVMLCEYLQMFPRYSDSTTIGGSSFEAHLNHAVAAMRAGKC
                     EMALISYGSTQLSSRGRVLGTGGRPATIPESTYESPYGNVLVGAYAMAARRHMHQFGT
                     TSEQLAEIAVVTRRHAGLNPLAMYREPITVQDVLNSRMIADPLHLLDCCVVSDGGGTV
                     LVTTEERARDLRQLPIYVLGSSEGHTHAHISQMPDLTVTPAAMTGPRAFAEAGVSPED
                     IDMAMIYDSFTITVLLLLEDLGFCKKGEGGAFVQGGRIALGGQLPINTDGGGLSSNHP
                     GMRGIFLIIEAVRQLRGQCGARQVEGAKLAVAHGSGGLLSSQATTILSSI"
     gene            3897..4859
                     /locus_tag="VGT44_01835"
     CDS             3897..4859
                     /locus_tag="VGT44_01835"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEV2579565.1"
                     /translation="MTVLQIYLFGKFCVRRDGQELEGLDARKVQELFCYLLLHRDHAL
                     SRETLASLLWPETTTALSKKNLRQALWQLQSALGSQNESMSDRVLIVEPEWIRLNPEA
                     NFWLDVSTLENTFQLVQKVPGQNLDSSVAQLLQSAAQLYQGPLLEGWYQDWCLYDRER
                     LQGIYLSLLDKLMGYCEAHRDYETGLHFGMRILCYDRARERTHRRLMRLHYLNGDRAS
                     ALRQFEQCSSALEEELNVSPSKGTLVIYEQILADQLDEPVAAIASSETSSEIAEFLLP
                     GILHRLTQLHTSISDLQKQLEQSIQVVEQALDRHAVLTKARPVA"
     gene            5037..5285
                     /locus_tag="VGT44_01840"
     CDS             5037..5285
                     /locus_tag="VGT44_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579566.1"
                     /translation="MDLVEAETQSFIVKVWVEDSAKDTQQGVWHGHITHVPSHERRYL
                     KNLGEIEDFIAPHLEEMGVQLSRSWRLRRWLMRLLWQR"
     gene            5348..5956
                     /locus_tag="VGT44_01845"
     CDS             5348..5956
                     /locus_tag="VGT44_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579567.1"
                     /translation="MTTTMKKAVTDRVDPNEPPTPVQTPAFLKSLTVIGNLETDLEPL
                     LSGYLSANIHDEVEPYSRYVSIIDANEYWYVTARWALNGTVRKMICGNWCVRLFMESL
                     GEDKLDLELSNDEGLIPLDPCGNGQYEAHFTVKPGTVRTEHCGTPFQPVLSVTYLTSC
                     KVRFRSDAEEAATPEYKRFQAGPIAGIARFPITQFIMEEIEA"
BASE COUNT         1441 a         1668 c         1712 g         1195 t
ORIGIN      
        1 tagccgcgca gcacggcgcg ctgttctgag cgaagcgcca gcgcgcgacg aagtgcggcg
       61 gcgcaggtcg gacagtgcgg cagatggacg cgcatctgta cgacgaggtg ctgatcctgg
      121 cggtccctgg ggacataggt ctgctcgaat acatcctctg accgggtact cgccagatct
      181 gccaggtcat cgagcaaacg cgaacagtgc gaactgagtt cgcacggtgg caaggtgccc
      241 ggtcggctat cgtacattat cgtccctcct gctcaagctg ggaatagaga cgttgaaacg
      301 actggcgggc gcgcgataag cgactgcgca cggcggaaag cgtgtcttgc agcgtatcgg
      361 caatttcgcg gtaggaaaac ccctcggcgt cgagcaaaat acacatgaca gaagtaggag
      421 gaagttgcgc catgacacga tcgattaaat cgcgctgcac aacttgctcc tcaaagcgtt
      481 ctccactgat gagactttct tcgccagggt catcaaacac atcatcgtcg ttgccctgcg
      541 gcgtagcaaa gggcatgacc gagacatgtt ttcgcctcct ccactcactg cgcacctggt
      601 tcaaggcgat acgatacagc cagggggcga agcgtagttc atctccattc atacgcggca
      661 acgcccggta tgctgccaga aacgtttcct ggcagaggtc ctgcgttaac tggctgtcct
      721 gcgtcaggct atacaacatt cgtcggatgc gcggctcata gtacacgacc agcgcctcga
      781 atgctgccat tgaccctgta cacgctgcga gggtaagctg gggctcgttt tccagtacgt
      841 tcatacggct cccactattc agttgaaact gaatcagttc ctcttgagag ggataatgca
      901 accaatagac gttttgtttc gataaatggt tcccgtcata ggacattgcg ggccataaaa
      961 aaagatgcta ccccatacac attatagggt agctctgcac atttcatcta tgaactagct
     1021 cacaatttgc tagcgaggcc ggaaacgcgg caacgaaatt tcgtcagaga ccgggtcaaa
     1081 cacaaccacg acaggcaaat caacacgcag gtcgtcgggt gcaatatcga taacattgct
     1141 catcatgtgc ggcccctcct caagctcgat cagggccacg ttataaggaa catcatcacg
     1201 aaattccggc gatttgttct ggcgaataac ggtatacgaa tagaccacgc cgcgcccgct
     1261 cgcctcgatc cagcgcacct gctgcccgcc gcaacgcatg caatatagtc gggggtaaaa
     1321 ctggctctgc tggcaagcat cgcaatattg cagcagcaac ttgccctgcc tgcaaccctc
     1381 ccagaatggg cggttcacct cggtgacggt cggcagtggt ctggcacgtg ttgtatccgg
     1441 cataaagacg gctccctggt tctacacagt aaggttgtat ccctcatgat tgtatcacgt
     1501 atggtgatgc ttgccatcgc cctggctact caggtataat gggagctaat gaagatcaag
     1561 ccccacgacg tggggaaagc gcattaatca aggatgagag cataggagtt cgctgccatg
     1621 tctacccccc caaaccctcg tcgtgagcat cccagtacct atgtagtgcc ggaccgctcc
     1681 accgacgaaa acactcgtct ccaattgcag gaccagatga tcaccgcgag catgggtggg
     1741 gtgctaccgg aacagtcaga ccgggtacag tttaagcgca tactggatgt gggctgcgga
     1801 accggaggct ggctgatcga gtcagcgaaa acctatccga cgatgactga actggtgggg
     1861 gtggacgtga gcgccaaaat ggtcgagtat gcgcaagcac aggcggagga gcagcaggtc
     1921 agtgatcggg tgcagtttcg cacgatggac gccttgcgca tgctggaatt tccggtcggc
     1981 tactttgacc tggtgaatca acgcttcggg attagctacc tgcggaagtg ggactggccc
     2041 aaactgctcc aggagtatca acgagtaact cgatcgggtg gggtgatccg catcaccgaa
     2101 accgatatca tcacggagac gagcagtcct gccttgctgc gtcttgatct cctgctcatg
     2161 gaagcgcttg cccaggcggg acatcttttt accgcggaaa gggacgggct gaccagccag
     2221 ctagccaact tgatgcgcca gcatggactt cagaatatac aaacccgcgc ctgtgacctc
     2281 gtgtatcgag caggtactcc agaaggacat atgtttcacg aagatatgaa gcaggtctat
     2341 cgaaactttc tgcctttttt gcggaagtgg acacgtgtcc ctgaagacta tgaaacgctc
     2401 tatcaacaaa tgctgagtga gacccaacag gcagacttcg tggcaacgtg gaatgtgttc
     2461 accgcatggg ggaacgcgcc ggtgaaaaca gctcgattcg ccccgtccac catataaaac
     2521 agcataaaaa taatgattcc agctcctcca gagagtatca cggagtgcag agtatgccat
     2581 cgtacaaagg acgcattgtc atcgcggggg ttgccgaatc ggactatggc cgcgtaccgc
     2641 atctgacgga gctggagcta catgcgcagg cgctgcagcg tgtgttggag gagagcggag
     2701 cgcgcaaaca ggatatcgat ggcgtcttct gtagctcgca catgatgggc atgccgacgg
     2761 tcatgctgtg cgagtacctg caaatgttcc cgcgctatag cgatagcacc accatcggcg
     2821 gctcttcgtt cgaggcgcat ctcaaccacg cggtggcggc gatgcgcgcc ggtaagtgcg
     2881 agatggcgtt gattagctac ggcagcacgc agctttccag ccgggggcgc gtcctgggta
     2941 caggcgggcg accggccact atccccgaat caacctacga aagcccgtat ggcaacgtgc
     3001 tggtcggcgc ttacgccatg gcggcgcggc ggcacatgca tcagttcggc acaacctcgg
     3061 agcaactggc ggagattgcc gtcgtgacgc gccgccacgc cggactcaac cctctggcga
     3121 tgtaccgcga gccaatcacc gtgcaggatg tgctgaattc gcgcatgatc gccgacccgc
     3181 tgcacctgct cgactgctgc gtagtgagcg acggcggcgg aaccgttctc gtcaccaccg
     3241 aggaacgcgc cagggacttg cggcagctgc ccatctacgt gctgggcagc agcgaaggtc
     3301 acacccacgc gcacatttcg cagatgcccg acctgaccgt caccccggca gccatgacag
     3361 ggccgcgcgc cttcgccgag gctggagtat cccctgaaga catcgatatg gccatgatct
     3421 acgactcctt caccatcacc gtgctgttgc tgctggaaga cctgggcttc tgcaaaaagg
     3481 gcgagggcgg cgcgttcgtg cagggcggac gcatcgcact gggcggccag ttgccaatca
     3541 ataccgacgg cggcggccta tcttccaacc acccagggat gcgcggcatc ttcctcatca
     3601 tcgaagccgt gcgccagctg cgcgggcaat gcggcgctcg ccaggtagag ggcgcgaagc
     3661 tggctgtagc gcacggtagc ggcggtctgc tatcgtcgca ggcgacgacg atattgagca
     3721 gtatataacc cacctgagcg agcataacga cgaggcagaa ccgtacaata tccaatattt
     3781 agacgcgaaa aaagtactat gctatcgtac ttgcaatgga tgtaagttat gcaaatgtgt
     3841 atgagagcca cgtacttatt cgattagata ggtcaccaga aagggagacg ctatctatga
     3901 ccgtcctgca aatctatctc ttcggcaaat tttgcgtgcg gcgtgatggg caggaacttg
     3961 aaggtctcga tgctcgcaag gtgcaggaac tattttgcta cctgctgctg catcgcgacc
     4021 acgccctttc ccgcgagacg ctggccagcc tgctctggcc tgagacaacc accgcgttat
     4081 cgaagaagaa cctgcggcaa gccctgtggc agcttcaatc agccctgggt tcgcagaatg
     4141 agtcaatgag cgaccgcgta ctgattgtcg aaccagagtg gattagactc aatccagaag
     4201 caaatttctg gctggatgta tcaacattgg agaatacgtt ccagcttgtg caaaaagtac
     4261 ccggtcagaa tcttgacagc tcagtggcac agttactgca aagcgctgcg cagttgtacc
     4321 aggggccgct gctcgaaggc tggtaccagg attggtgcct gtatgaccgc gagcggctgc
     4381 agggcatcta tttgagcctg ttggataagc tgatgggcta ttgtgaggcg caccgcgact
     4441 acgagaccgg tctgcacttc ggaatgcgca tcctctgtta tgatcgagcg cgcgaacgga
     4501 ctcaccggcg cttgatgcgc ctgcactatc tgaatgggga ccgggcatcc gccttacgac
     4561 aattcgagca atgctcaagc gcgctagaag aagaactcaa cgtcagccct tcaaaaggaa
     4621 cactggtaat ctacgagcag attcttgcgg atcagctgga tgagcctgta gcggcaatcg
     4681 catccagtga aacctcaagt gaaatagccg aattcctact gcccggaatc ctgcatcgcc
     4741 tcacacaact ccatacatcg atatccgacc tgcaaaagca actcgaacag agtattcagg
     4801 ttgtcgaaca ggcgctggac cgtcacgcgg ttctgacaaa ggcccgccct gtcgcttaaa
     4861 aaagacacgc aggagacggc gaaaagatgc tccagagacg caaagtagta cgagcatttg
     4921 tacccccgag agacgtttgg agagacgccc gcatggtaca gttctcttgc acctgaaagg
     4981 cctgggcgag acaaacgaaa cctggcccca ggatcaacct ggatgagagg acgactatgg
     5041 atctggtaga agccgagacg caatcgttca tcgtcaaagt ctgggttgaa gatagcgcaa
     5101 aggacacgca gcagggagtc tggcatggcc acattaccca tgtccccagt catgagcgcc
     5161 gctatctcaa gaacctgggc gaaattgagg atttcattgc tcctcacctg gaagaaatgg
     5221 gtgtacaact cagtagaagc tggcgtctga gaaggtggct gatgcgcctg ctgtggcaga
     5281 ggtaatgtgt atctaccttc tggcaccagc aatcgtgtaa cgacaagttt tcagaaagag
     5341 agtgagcatg actaccacaa tgaaaaaagc ggtgaccgat cgagtcgatc cgaacgagcc
     5401 accaacgccg gtacagaccc cggcattcct caaatcactg actgttatcg gaaacctgga
     5461 aactgacctt gagccgctcc tgtccggcta cctcagcgcg aacatccacg acgaagtcga
     5521 gccatattcg cgctatgtca gcatcatcga tgccaacgag tactggtatg tcacagcgcg
     5581 gtgggcgctt aacggaacgg tgcggaagat gatctgcggc aactggtgcg tcaggctatt
     5641 catggagtcc ctgggcgaag acaaactcga tcttgagttg tccaacgacg agggtctgat
     5701 tcctctcgat ccatgcggca acggccagta tgaggcacac ttcaccgtga agccgggtac
     5761 ggtgcggacg gaacactgcg gaacgccctt ccagccggtt ttgagcgtga cctacctcac
     5821 gagctgcaag gtccggttca gatcagatgc cgaagaggcg gcgacgcccg agtataagag
     5881 gttccaggca gggccgatag ccggtattgc ccggttcccg atcacgcagt tcatcatgga
     5941 agagatcgaa gcctgaagcc gactcgtatc aatctcccgg tttcagtcga aattggaaga
     6001 gggaagagcg atgtca
//
LOCUS       DASZAK010000110         8876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_677170, whole genome shotgun sequence.
ACCESSION   DASZAK010000110 DASZAK010000000
VERSION     DASZAK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677170"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VGT44_01850"
     CDS             complement(<1..270)
                     /locus_tag="VGT44_01850"
                     /inference="COORDINATES: protein motif:HMM:NF044812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579568.1"
                     /translation="MDRIIEIRWHPYRIPLNREFVTAHSVMAVREGAIVEIITESGIV
                     GLGEIAPLAAFGRESLAAILARLPALAARLRKRTVEEALHTLEAAL"
     gene            complement(285..1103)
                     /gene="menB"
                     /locus_tag="VGT44_01855"
     CDS             complement(285..1103)
                     /gene="menB"
                     /locus_tag="VGT44_01855"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HEV2579569.1"
                     /translation="MAISWKNVGDYGDIIFEKAEGIGKITINRPEVRNAFRPETLTEL
                     IDAFTACREDPEIGVVILTGKGDKAFCSGGDQRVRGEGGYVGHDGVPRLNALELQKVI
                     KYLPKPVIAMVAGYAIGGGHVLHLLCDLTIAADNARFGQTGPRVGSFDAGWGATYMAR
                     VVGHKKAREIWYLCRQYSAQEALDMGLINTVVPLERLEEETIQWAREILEKSPIALRF
                     LKSAFNADTDGLAGIQELAGNATMLYYMTEEGKEGRDAFLEKRKPDFSRFPRLP"
     gene            complement(1252..2100)
                     /gene="menH"
                     /locus_tag="VGT44_01860"
     CDS             complement(1252..2100)
                     /gene="menH"
                     /locus_tag="VGT44_01860"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HEV2579570.1"
                     /translation="MMRKLIEVNGIRLGVELLGEPGARRTLVLLHGFTGSAAGWGSHL
                     DALAADNMRVIAFDLHGHGQSDAPADPERYSMEHCRADILAALRELGVGAGEAVLVGY
                     SMGGRIALYLALGGFFRALVLESASPGLASAAERAARRASDEALAVSIERDGVAAFVE
                     RWERLPLFASQHALPADRREALHAQRLRNSAMGLANSLRGVGLGAQPALHDRLPTLDL
                     PVLLMSGELDQKFSELAAQMAQLLPHAQWRSVPGAGHAVHLERPEEFDELVNDFVGAD
                     LARPGR"
     gene            complement(2160..3983)
                     /gene="menD"
                     /locus_tag="VGT44_01865"
     CDS             complement(2160..3983)
                     /gene="menD"
                     /locus_tag="VGT44_01865"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HEV2579571.1"
                     /translation="MMSESDQVQDRANPLYIYVNAFVDELQRAGVRHVVICPGSRSTP
                     LAMACASQPGLRVWMHVDERSAAYFGLGMAKKLGKPVALLCTSGTAAANFLPAIVEAK
                     LSHVPLLILTADRPHELRDNGAQQSIDQNRLYGSYVKWFVDVALPEATNEALRYIRTL
                     ANRAAGLACAVAAGPVHLNFPFREPLTPEPLTERELPASCDPIAWEGRPDNTPYVRVE
                     DAPLAVPSPERLARLAELANTTTRGLIIVGPFDNPALVEPLVRLARRLGFPILADPLS
                     QARCGGHDLEQVISSYDAFLRVDAFVERAAPELVLRFSAMPTSKPLLLYLKRYAACPL
                     IVIDGHGTWEEPTQLAAEVIHSDPPAFCQNLLAALGDQHEQGEPPAVDGGTWLATWQR
                     AERVTRQALQEAMQGFRQPFEGRVFTELARLLPDSATLYAGNSMPVRDLDTFFWSSKR
                     RVRMMANRGANGIDGVVSSALGASAVSGEEEPTVLVIGDLSFFHDLNGLLAARLHRLN
                     LTIVLVNNDGGGIFSFLPQAAYPEHFEQLFGTPTGLDFRPVVQMYQGHFQRVDNWEEF
                     GEALLEGFSAGGLRVIEVPTERASNVQMHRELWQALAAIDP"
     gene            complement(3986..5464)
                     /locus_tag="VGT44_01870"
     CDS             complement(3986..5464)
                     /locus_tag="VGT44_01870"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF005459.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HEV2579572.1"
                     /translation="MNTTTLHAIPRLWPVPCGKEQLGTLLRKTLRAAADRSARSGRAV
                     LASITLPVERIDAIQVFAGSRLAGFGTRFFWERPSEQTAFVGIGAAARIETHGASHFA
                     SAAAAWRALLDDAVIDTLPNAFPLPGGGPVAFGGFAFDPLSLHTPLWEGFPDGLLTLP
                     RLLLSYAAEEATLTINRQVQASDDSEYEAEQVADDLARLWAGVGQPSLWQETTGGSNA
                     LTTQDLLPLSEWMQQVAETVKLIRQGIYEKVVLARGLRVTSESPFDIGAVLHRLRQEY
                     PGASIFAIQRGQRLFVGATPERLVRVQDRHIQTMALAGSAARGASEEEDRQLGEDLLR
                     SEKNKIEHIIVVERVREALTTLCARVSVAEMPRLLRLKNIQHLETPIVGELLPGKSIL
                     ETLGALHPTPAVGGLPGPEALERIRECEQLDRGWYAGPVGWVDADGNGEFAVALRSAL
                     LEGRQATLFAGCGIVADSDPQSEYAESCLKFQAMLRGLGSED"
     gene            complement(5612..6553)
                     /locus_tag="VGT44_01875"
     CDS             complement(5612..6553)
                     /locus_tag="VGT44_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579573.1"
                     /translation="MSYLLSCISTRHSLLKQHWKETRDQISCPVTVLGSRYKPEEDQG
                     GLLCLLDERGQVQSSVELPMPAGLARSENGVLVASLEAIHEVSADLSTVRSDVVSLPS
                     FNLLHSLSRTAQGYLVASTGMDAILEFTPQGEPIWSWWATEHGFEETPTGERRFLDTE
                     ADYRGIKYGTLAQTTHVNSAAELPDGTILASLFHQGMVIAIDRASGAWRSVLEGLDHP
                     HAVRVLAGDYFTVADTGQGRALLVHLEYGKGTIEAEVSAGTTWLQDCSYDYKHDHWIL
                     VDGKHSRVTLRAGTAGEKEVARFDLDPEWRLYEVLPL"
     gene            7039..8787
                     /locus_tag="VGT44_01880"
     CDS             7039..8787
                     /locus_tag="VGT44_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HEV2579574.1"
                     /translation="MSEATHDDRSLLHLGPGEGSKVEHIKVNSHHLRGQIAEELSGES
                     THFSEDQIQLIKFHGMYQQEDRDQRHARKEAREEKAYQFMIRSRIPGGALTAEQYLVE
                     DDLAGQYGNGTLRITTRQGFQLHGILKGDIRATIREINECLLSTLAACGDVNRNVMAC
                     PAPTASRAQARVQEIAHQIAMRLAPRSSAYHEIWIDGEKVETVEDPQEVEPLYGPTYL
                     PRKFKIGVAFPGDNCVDVYTQDIGLVAILDGGELAGFTVLIGGGMGMTHGKKETFPRL
                     GTPLCDVSVEQVLPLVEAIVTVQRDFGDRTNRKHARMKYVVEEQGVAWFRAEVEQRLG
                     YSLCDPRPVHLHDVEDHLGWHRQGDGRWFLGLHIDNGRIADTAARSLRTGLRQAIEQF
                     RPGIRLTGQQNILLTDLTEAQRAPLETLLREFDISTDPQAAGAYRFAMACPSLPTCGL
                     ALAEAERALPGVMQQIEADLAGLGLAGEPLSVRMTGCPNGCARPYMGDIGIVGRTKDV
                     YNIYVGGDWANTRMNTLYAPSIHLRDLAATLYPLLALWRDERLPLETFGDFCHRLGVE
                     QLQQRVSAKTGLGLSV"
BASE COUNT         1853 a         2866 c         2465 g         1692 t
ORIGIN      
        1 caacgccgcc tctaaagtat gcaacgcctc ctcgaccgtc cttttgcgca gtcgtgccgc
       61 cagcgccggc aaacgcgcca aaattgcagc gaggctctcc ctgccaaatg ccgcaagtgg
      121 cgctatttcg ccaagaccaa caatgccact ctcggtgatg atctctacga tagccccttc
      181 gcgaaccgcc atgacgctgt gcgcggtgac gaattcgcga ttcaagggga tgcgatacgg
      241 atgccagcgt atctcgataa tcctgtccat cttgccttaa cagcctatgg caggcgcgga
      301 aacctggaaa agtccggctt gcgtttctcc agaaaggcat ctctgccctc cttgccttcc
      361 tcggtcatat agtagagcat ggtagcatta ccggccagtt cttgaatgcc cgccaggcca
      421 tcggtatcgg cattgaaggc cgatttcagg aagcgcagcg cgatcggact tttctccagg
      481 atttctctgg cccactgtat ggtttcctct tccaaccgtt ccagtggaac tacggtattg
      541 atcagaccca tatccaacgc ttcctgggcg gaatactggc ggcagagata ccagatctcg
      601 cgcgctttct tgtgacccac gacgcgcgcc atataagttg cgccccaacc ggcatcgaag
      661 ctgcccaccc tggggccggt ttgcccgaac ctggcgttgt cggcagcaat cgtcaggtcg
      721 caaagcaggt gcagcacatg cccgccgccg atggcatagc ccgcgaccat cgcaatcacc
      781 ggcttgggaa gatatttaat gactttttgc agctcgagcg cgttcaagcg cggcacaccg
      841 tcatgcccaa catacccacc ctcgccccgc acacgctgat cgccgccaga gcagaacgcc
      901 ttatcgccct tgccggtaag gataaccacg ccgatctccg ggtcttcacg gcaggccgtg
      961 aaagcatcga tcaattccgt cagggtttcg gggcggaaag cgttccgcac ctctggccgg
     1021 ttgatcgtaa tcttcccgat gccctcggcc ttttcaaaaa tgatgtcgcc gtaatcccca
     1081 acatttttcc aggaaatagc catgttattt tctcctttgc tacaacaaaa ataatcgtag
     1141 gtgccgatta tgaacttgaa cgaccaacat cggcaagaga atgtcatcct tcgctgcgct
     1201 caggatctcg gtcctcagcg tattagaccc ttcgctgcgc tcagggtgac attatcggcc
     1261 tggacgcgct aaatcggctc ctacgaaatc attaaccaac tcatcgaact cctctggccg
     1321 ttccaggtga accgcgtggc ccgcgcccgg cacactccgc cactgcgcat gaggaaggag
     1381 ttgcgccatc tgcgcggcaa gctcgctgaa tttctgatcg agttccccgc tcatcagcag
     1441 gacaggcaaa tcgagcgtgg ggagccgatc atgcagcgcg ggctgcgcgc ccaggccgac
     1501 accgcgcagg ctattggcca gtcccatcgc cgaattgcgc aggcgctgcg cgtgcaatgc
     1561 ctcccggcga tcagcaggca gcgcatgctg gctggcaaag agcggcagtc gctcccatcg
     1621 ctccacaaat gccgcaactc catcgcgttc gatgctcacc gccagcgcct catcgctggc
     1681 gcgccgcgcc gctcgttcag cagcgcttgc cagccccggc gatgcgctct ccaatacgag
     1741 cgcgcgaaaa aatccgccga gcgccaggta caacgcgata cgcccgccca tcgaatagcc
     1801 aaccaacacg gcctcgcccg cgccaacgcc gagttcgcgc agcgcggcca ggatatccgc
     1861 ccggcaatgc tccatactat agcgctctgg atcggcgggc gcatccgact gcccatgtcc
     1921 atgcagatca aaggcgatta cacgcatgtt gtcggctgcc aacgcatcga gatggcttcc
     1981 ccaaccagcc gcgctcccgg taaagccatg cagcaaaacc agcgtccgcc gcgcccccgg
     2041 ctcgcctagc agctcaacgc ccagccgaat cccattcact tctatcaact ttctcatcat
     2101 caatagctcc tgaatacccc acatatacaa tcttcgtccg cccctacggt ccatatatgt
     2161 tacgggtcaa ttgcggcaag cgcttgccac agttcgcggt gcatctgaac attgctggca
     2221 cgctcggtcg ggacctcgat gacgcgcaag ccgccggcgc tgaatccttc cagcaacgcc
     2281 tcgccaaact cctcccagtt gtccacacgt tgaaaatgac cctggtacat ctgcacgacc
     2341 gggcggaaat ccaggccggt cggtgtgccg aaaagctgct cgaagtgttc ggggtaggcg
     2401 gcctggggca ggaacgaaaa aatccctcca ccgtcattgt tcaccagcac aatcgtcaga
     2461 ttcaggcggt gcagccgcgc agccagcagc ccgttgagat catgaaagaa cgacagatca
     2521 ccgatcacca gcaccgtcgg ttcttcctcg ccactcacgg cgcttgctcc gagcgcgctg
     2581 gagactaccc cgtcgatgcc gtttgccccg cgattggcca tcattcgcac acgccgcttg
     2641 ctgctccaga aaaaggtatc caggtcgcgc accggcatac tattgccagc gtagagcgtc
     2701 gcgctatcgg gcagcaggcg agccagttcg gtgaagaccc ttccctcgaa cggctggcga
     2761 aacccctgca tagcttcctg tagggcctgc cgcgtgaccc gctcggctcg ctgccaggta
     2821 gcaagccatg tgccgccatc aactgctggc ggctcgcctt gttcatgctg gtctccaagc
     2881 gcggccagca ggttctggca gaaagcaggc ggatcagagt gtattacttc ggcggcaagt
     2941 tgggtcggct cttcccacgt cccatgccca tcgatcacga tcagggggca ggccgcgtaa
     3001 cgctttaagt ataacagcag tggcttcgaa gtgggcattg cgctaaaacg cagcaccagt
     3061 tccggcgcgg cgcgttccac gaacgcatcg acgcgcagga acgcatcata gctggaaatg
     3121 acctgttcca ggtcatgacc gccgcagcgc gcctgggaga gcggatcggc aagaatggga
     3181 aagccaagcc gccgggccag ccgcacaagt ggctcaacga gcgccgggtt atcgaacggg
     3241 ccgacgatta tcaggccgcg cgtggttgtg ttcgccagtt cagcgaggcg cgcgagacgc
     3301 tcaggtgatg gcacggccag gggagcgtcc tctactctga cataaggcgt gttatctggc
     3361 cgtccctccc aggcgatggg atcgcacgag gccggtaact cccgctcagt gagcggctcc
     3421 ggcgtcagcg gttcgcggaa tgggaagttg agatggaccg ggcctgcggc aacggcgcaa
     3481 gccagccctg ccgcgcggtt ggcgagtgtg cgaatgtaac gcaacgcctc gttggttgct
     3541 tccggcaacg ccacatcgac aaaccacttt acatacgagc catacagccg gttctgatcg
     3601 atggactgct gcgcgccgtt gtcacgcagc tcgtgagggc ggtccgccgt caggatcagc
     3661 agcggcacgt gcgataactt cgcctcgacg atagcaggca gaaagttcgc ggctgccgtc
     3721 cccgacgtac acagcaaagc aaccggcttc cccaactttt tcgccatgcc cagcccgaag
     3781 taggccgccg aacgctcatc cacgtgcatc cacacccgca acccgggctg cgaggcgcag
     3841 gccatcgcca ggggagtcga gcgcgaccct gggcagatca cgacatgccg caccccagct
     3901 cgctgcagtt catccacaaa ggcgttgacg tagatataga gaggattagc acggtcttgt
     3961 acttgatctg attcgctcat catactcagt cttcactccc cagcccgcgc agcatcgcct
     4021 ggaacttcaa gcaggactcg gcatattcgc tctgcggatc agaatcggcc acaatgccgc
     4081 agccagcgaa aagcgtggcc tgcctgcctt caagcaaagc cgagcgcaac gccaccgcga
     4141 actcgccatt gccgtccgcg tccacccagc cgaccggccc cgcgtaccag ccgcgatcaa
     4201 gctgctcgca ttcccggatt ctctcaagcg cctccgggcc aggaagtcct ccaacagcag
     4261 gtgtaggatg cagcgccccc agcgtttcga gtatcgattt ccccggcaga agctcgccaa
     4321 cgatgggcgt ctccaggtgc tggatatttt tgagacgcag caggcgcggc atctcagcca
     4381 ccgataccct ggcgcagagg gtcgtcaggg cttcgcgcac ccgctcaacg acgataatat
     4441 gttcgatctt atttttctca gagcgcagca agtcctcgcc aagctgccgg tcttcttcct
     4501 cgcttgcgcc gcgcgccgcc gaacctgcca gcgccatcgt ctggatatgc ctgtcctgta
     4561 cgcgcaccag ccgctcagga gtcgcgccca cgaacaggcg ttgcccgcgc tggatggcaa
     4621 agatggacgc gcccggatat tcctggcgca ggcgatgcaa aacggctccg atatcgaaag
     4681 gcgactcgct ggtaacgcgc aggccacgcg ccaggacaac cttttcatat attccctgcc
     4741 ggatcagctt caccgtctcg gccacctgct gcatccactc cgatagcggc agcaagtcct
     4801 gggtagtcaa tgcattggaa ccacccgttg tttcctgcca aagcgacggt tgcccgaccc
     4861 cggcccataa gcgcgcgaga tcgtctgcaa cctgctccgc ttcatactca ctatcatcgg
     4921 acgcctgcac ctgcctgttt atcgtcaacg tcgcttcctc cgccgcatag cttagcagca
     4981 ggcgaggcag cgtgagcagc ccatcaggaa atccctccca cagaggagta tgaaggctga
     5041 gcggatcaaa ggcaaatccc ccaaaagcga caggtccgcc tccagggagg gggaatgcat
     5101 tgggtagtgt gtcgatgacg gcatcgtcaa gcaaggcgcg ccaggcggct gccgcgcttg
     5161 cgaagtgcga tgcgccatgc gtttcgatac gcgcggctgc gccaatgcca acaaaagcgg
     5221 tctgctccga cggccgttcc cagaaaaagc gcgtgccaaa tcccgccaac ctggagccag
     5281 caaacacctg gatcgcatct atccgctcaa ccggcagcgt aatactggcg aggacagcgc
     5341 ggccagatcg agcagatcga tcggcagccg cgcgcagcgt ctttcgtagc agggtaccta
     5401 gttgctcttt gccacaggga acaggccata gccggggaat agcatgtaaa gttgtagtat
     5461 tcatagcttg atatttctgc caaatcgact catgggtttc tttacttaaa attgtaggag
     5521 tctctctatt gacaggtcaa gaccagaagc caggagccag gggagggaag cagcttgacg
     5581 tcgttgtcgt gcttcaattg tgacaactct gttagagtgg cagcacttcg tatagacgcc
     5641 attccgggtc caggtcaaac ctggccacct ctttttcgcc tgccgtgccc gcgcgcaggg
     5701 tcacgcgcga atgcttgcca tccaccagta tccagtgatc gtgtttgtag tcgtaagaac
     5761 aatcctgtaa ccatgtcgta cccgctgaca cctccgcttc aatggtgcct tttccatatt
     5821 cgagatgtac cagcagcgct ctcccctggc cggtgtcggc aaccgtgaag tagtccccgg
     5881 ccaggacgcg taccgcgtgg gggtgatcga gtccttcgag gacagaccgc cacgcgccgc
     5941 tcgcgcggtc aatcgcaatc accatgccct ggtgaaacag ggaggccagg atcgtgccgt
     6001 ccggcagttc ggcagctgaa ttgacgtgcg ttgtctgcgc cagcgtgcca tacttgatgc
     6061 ctcgataatc tgcctcggta tccagaaaac gccgctctcc ggtgggcgtt tcctcaaaac
     6121 cgtgttcggt cgcccaccaa ctccagatcg gctctccctg cggtgtaaat tccaggatgg
     6181 catccatgcc ggtactggca accaggtagc cctgcgccgt gcgcgatagc gaatgcagca
     6241 ggttgaacga tggcaggctg acgacatcgc tcctgaccgt tgagaggtcc gcgctaactt
     6301 cgtggatggc ctcaagcgat gcgaccagca ccccgttttc agaccgcgcc aatcctgccg
     6361 gcatagggag ttcaacgctg ctctgtacct gccctcgctc gtcgagcaga cacagcagac
     6421 caccctgatc ttcctccggc ttgtaacgac tccccagcac ggttactgga cagcttatct
     6481 ggtctcgtgt ctccttccaa tgctgtttga gcagagaatg cctcgtcgag atgcaactga
     6541 gcagatagct catgtggtga ttcctcctat tctcccgtcc tcgccgggat tgcacgaaat
     6601 ccttgaacgc cctcgtaggc gccgatttat cgtgcgcatc gccgatttat cggcctgtgg
     6661 agcgcgctga caatccccct tggccgataa atcggcgatg cgcacgataa atcggcgcct
     6721 acgggatggc cggacttgct cgtgaagctt cattatcggc taatacgcac attggttcac
     6781 aatacatgtc ctggccgata aatcggcggt gagcgcgata aatcggctcc tacggatttc
     6841 atacaaccta ccaggtcagg gttgacaaaa ctgtttcttc caccctatag tagtaagtag
     6901 aagaacatta ctttgttgat aaactatatc aacaattcgc caccaaaagc aaggggtcag
     6961 aattaccatc tccgagggag aacagatggt aacaggtcaa cgctgcttct ttcccccggc
     7021 gcgagaggag atatactcat gagcgaggct acccatgacg atcgttccct gctgcatttg
     7081 gggccgggtg agggcagcaa agtcgagcat atcaaggtca acagccacca cctgcgcggc
     7141 cagatcgccg aggaattgtc aggcgagtct acccactttt ccgaggatca gatccagctc
     7201 atcaaatttc atggcatgta ccagcaggaa gaccgcgacc aacgccacgc ccgcaaagag
     7261 gccagagaag agaaggcgta tcaattcatg atccgctcgc gcattcccgg cggcgcgctg
     7321 acggcggaac aatatctcgt cgaggatgac ctggcaggtc aatatggtaa cggcaccctg
     7381 cgcataacca cgcgccaggg cttccagttg cacggcatcc tcaaaggcga tattcgcgcc
     7441 acgattcgcg agatcaacga gtgcctgttg tccacgctgg ccgcctgcgg ggatgtgaac
     7501 cgcaacgtga tggcctgccc cgcgccgacc gccagccgcg cgcaggctag agttcaggaa
     7561 atcgcccatc agatcgcgat gcgcctggcc ccccgcagca gcgcctatca tgaaatctgg
     7621 atcgacgggg aaaaggtgga gacggtcgaa gacccgcagg aggttgagcc gctctacggt
     7681 ccgacgtatt tgccgcgtaa gttcaagata ggcgtcgctt tccccggcga taactgcgtc
     7741 gatgtctaca cgcaggatat cggcctcgtg gccatcctgg acggcggtga actggccggc
     7801 ttcaccgtcc tcatcggcgg cggcatgggc atgacgcacg gcaaaaagga aaccttcccg
     7861 cgcctgggaa cgccgctctg cgatgtttca gtcgagcaag tgctgccact cgtagaggct
     7921 atcgtgaccg tacagcgcga ttttggggac cgcacgaacc gcaaacatgc gcgcatgaag
     7981 tacgtcgtag aggagcaggg cgtcgcgtgg ttccgcgccg aagtcgagca gcgcctgggc
     8041 tactctttgt gcgaccctcg ccccgtccac ctgcacgatg tcgaagatca tctcggctgg
     8101 cacaggcagg gagacgggcg ctggttcctc ggcctgcata tcgataacgg gcgcatcgca
     8161 gataccgccg ccagaagtct gcgaacagga ctgcgccagg ccatcgaaca attccgtccc
     8221 ggtatccgcc tgaccgggca gcagaatatc ttgctgaccg acctgaccga agcgcagcga
     8281 gcgccgctcg aaaccctgct gcgcgaattc gacatctcca ccgatccaca ggcagccggc
     8341 gcctatcgtt tcgccatggc ctgcccttcg ctgcccacct gcggcctcgc gctggccgag
     8401 gccgaacgcg ccctgccggg cgtgatgcag cagatcgagg ccgacctggc cgggctgggc
     8461 ctggcaggcg agccgttgag cgtgcgcatg accggctgcc ccaacggctg cgcgcgcccc
     8521 tacatgggcg atatcggcat cgtgggccgc acaaaggatg tctacaacat ctacgtcggc
     8581 ggcgattggg ccaatacgcg catgaacaca ctctacgcgc cctccatcca tctcagagac
     8641 ctggcagcga ccctctatcc gctcctcgcc ctgtggcgag acgaacgcct gcccctcgaa
     8701 accttcggcg atttctgcca ccggctcggc gtagagcagt tgcagcagcg agtcagcgca
     8761 aagacgggat tgggcttaag tgtatgaaat aaccgtaggt gccggtttac cgcgcacacc
     8821 gccgatttat cggccccgat gggagttatc ggcaggcatt caggccgata atggtt
//
LOCUS       DASZAK010000111         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_109426, whole genome shotgun sequence.
ACCESSION   DASZAK010000111 DASZAK010000000
VERSION     DASZAK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109426"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1293
                     /locus_tag="VGT44_01885"
     CDS             115..1293
                     /locus_tag="VGT44_01885"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2579575.1"
                     /translation="MEPSFSEEDGSSAQLEAIFRTQFAGLYRYIYRQVRHAAIAEDLT
                     SAVFLKALRWLRQDRSPESVKGWLYATARSILADYWREEAQVNLLPLEEVEEMPLLSE
                     ESDEQMRSLQARIQRLLGGLSARERDVLTLRYFQGYSAAEIGQVLGLSAKHVRVLQLR
                     ALRRAALLEAGERSGHVESPTMPYNEQALRVLELTKEEASASNHNYLGTEHLLLGILR
                     EGSAAGELIDHGITLERMRGGILLILRGLPQGTAGSQLGFTPRAQHVLVLAGEEAQRL
                     GETAISPQHLLVAILREGQGIAAQLLQVSGVSLEQVGETTRISIASDTEGLPITLPAD
                     FQEALQQHPAANAMFEKLSYSKKKSLVDHIEHADGEAARSRQVEKAIELLEQIHQQRP
                     "
     gene            1636..2061
                     /locus_tag="VGT44_01890"
     CDS             1636..2061
                     /locus_tag="VGT44_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579576.1"
                     /translation="MTQTNEEKPSLADGLLPHNLANPYHLYQELRENDPVYWDERANS
                     WVLTRYSDVAAALRDARFSASDFMTDTSWIPDEMLPMLEPIIRPLTRQMLFMNPPDHT
                     RLRGLVARAFTPRMVEGLRPAIQQIAAELLDAVEAKGRI"
BASE COUNT          489 a          620 c          642 g          436 t
ORIGIN      
        1 caccgtcgtc acatgtcgtc caatccaccg acgtgtgacg cttcactaca tatgtgtaat
       61 gctgcactgt ataacaaagt ggaagaacga aatgggaagg agcaagatgc aggcatggag
      121 ccttcattct ctgaagaaga cgggagttct gctcagctgg aggccatctt tcgcacacag
      181 tttgccggtc tctaccgcta catctaccgg caggttcgcc atgccgccat cgcggaagac
      241 ttgaccagcg ccgtctttct caaagcgctg cgctggctcc ggcaggatcg cagtccagag
      301 agcgtgaaag gctggctcta tgcaacagcg cgcagcattc tcgcggacta ctggcgcgaa
      361 gaggcgcagg tcaacctcct gcctcttgaa gaggtggaag agatgccact gctttctgag
      421 gagagcgatg agcagatgcg gtcattacag gctcgcatcc agcgcctgct tggtggattg
      481 tccgcgcgtg aaagggatgt cctgaccttg cgttacttcc agggatatag tgcagcagag
      541 atcggccagg tcctgggtct gagcgcgaag catgtgcgcg tcttacagtt gcgcgccctg
      601 cgccgggctg ccttacttga ggccggagaa aggagtggtc atgtggaatc accaacgatg
      661 ccatacaatg agcaggcgct tcgcgtcctg gaactgacga aggaggaggc cagcgcctcc
      721 aatcacaact acctcggcac cgagcatttg ctgctcggca tcctgcgcga gggcagcgcc
      781 gcgggagagt tgatcgatca cggcataacg ttggaacgta tgcgcggcgg aattttgtta
      841 attctcagag gacttcccca gggaacggca ggctctcaac taggtttcac tccgcgcgcg
      901 cagcacgtac tcgtcctggc cggcgaggaa gcccaacgcc tgggggaaac tgcaatcagc
      961 cctcagcatc tcctggtggc aatcctgcgc gaagggcagg gcatcgccgc ccagttgctc
     1021 caggtctcag gagtgtccct ggagcaggtg ggagagacga ctcgtatcag catagcatca
     1081 gacacggagg ggctgcccat caccctgccc gcagactttc aggaggcgtt gcagcagcat
     1141 ccagcagcca atgccatgtt tgagaaactt tcgtactcca agaagaagag tcttgtagac
     1201 cacatcgaac atgccgatgg ggaagctgca agaagtcggc aggtggaaaa agccatcgag
     1261 ctactcgaac agattcatca gcaacgcccg tgacgtaaaa ccgtctcggt aatacctcgt
     1321 aggcgccggt tcaccgtgcg caccgccgat tgatcggcct gtggagcgtg ttgacaaccc
     1381 gtggggccga ttatgaactt ttcctaattc ctccggccat ccgagacggg aggacgaggg
     1441 cgcccgcaag aatttgttaa tctgcataat tggcggtgcg cgcagtgaac cggcgcctac
     1501 gaattggtcg ccctctggaa cctcatcaat gcaaaaattt agcggatacc cgtccttgta
     1561 accaccctgt acgctgcctc gtataattga cgatgaagca gcgaagaagg tctctatagc
     1621 tcaaggagat atccaatgac tcagacgaac gaagagaaac ccagcctggc cgatgggctg
     1681 ttgccgcaca acctggccaa tccttatcac ttgtaccagg agctacgtga gaacgatccg
     1741 gtctattggg atgaacgagc caatagctgg gtgctgacgc gctatagcga tgtagcggcg
     1801 gcgctgcgcg atgcgcgttt ctccgcctcg gatttcatga ccgacaccag ctggatacct
     1861 gacgagatgc ttccaatgct ggagccgatc attcggcctc tcaccaggca gatgctcttt
     1921 atgaacccgc ccgaccatac gcgcctgcgc gggctggtcg ccagggcgtt cacgccgcgc
     1981 atggtcgagg gcttgcgacc ggcaatccag caaatcgccg ctgaattatt ggacgcggtt
     2041 gaggcaaagg ggcgcatata actgattcgc gagtatgcct ttccgctgcc tgccatcgta
     2101 atcgccacca tgttaggcgt gccgccggag gatcgcgacc agtttaacga gtggtccgaa
     2161 caatttggtt cgctactgga cggcggc
//
LOCUS       DASZAK010000112         2546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_286413, whole genome shotgun sequence.
ACCESSION   DASZAK010000112 DASZAK010000000
VERSION     DASZAK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286413"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VGT44_01895"
     CDS             <1..353
                     /locus_tag="VGT44_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908056.1"
                     /note="activates fatty acids by binding to coenzyme A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV2579577.1"
                     /translation="EGYFYVIDRSKDMIKASGFNIFPREVEEVLLLHPAVQEAAVIGV
                     PHEYRGETVAAVIVLKPGFAPSKDTREDILAYCKKELTPYKVPKFIEFRESLPKTLIG
                     KVLKRELRETFKPS"
     gene            456..2240
                     /locus_tag="VGT44_01900"
     CDS             456..2240
                     /locus_tag="VGT44_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2579578.1"
                     /translation="MSTTMKPTEAGTAFLTTPVSENADKIFTLEQRDEEQRWIAESAA
                     TFAQREILPNVEAIDHQEPGLMPSLLKKAGEQGLLSIDVPEAYGGAELGLLTSALVAS
                     EFREGSFGTAVGAHTTIGTLPIVFYGTEAQKQFYLPKLATGEWISAYALTEPGVGSDA
                     MGLNTRAELSEDGKYYILNGTKQWISNAGFADVMIVFARIGDARPSAFIVETSWPGIS
                     TGAEERKMGIKGSSTRQVYFENAKVPVENMLGEIHKGYKIAFNILNIGRLKLGAGAAV
                     GSRNALNLAAAYTTERKAFGKFLHEFGLIQKKLARMAAETYAAESEAFRTAANIEQAR
                     RSAGDNTEAAFKAIEEYAIEASIAKVHGSEVLAYVVDEGVQIFGGYGFMQEYPIEKAY
                     RDARIQRIYEGTNEINRLVTSGTLFRRVMSGKLDLLSRIPVIEARVKSGQAPDVAGEA
                     IPAELRSAVNALERAKDATIYAAMRIAMKYMQALEQEQEFIEYLANLLIDLYAIDSAL
                     ARATQAVRRGDANSATHVKLAQLATWLAFSRMRLNLDQMIMTNMNEDQVEKELARVRA
                     YLGDYLINGVTMQRELAAIVVEKQGYPL"
     gene            2255..>2546
                     /locus_tag="VGT44_01905"
     CDS             2255..>2546
                     /locus_tag="VGT44_01905"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908053.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV2579579.1"
                     /translation="MSEAVIVSAVRTPIGRANKGVLKDVRADDLAAFAVKAAVDRVPQ
                     LDRSLIEDVILGCAFPEGEQGMNLARLVGALAGLPETAGGVTVNRFCASSLQA"
BASE COUNT          614 a          675 c          716 g          541 t
ORIGIN      
        1 aggagggtta cttctacgtc attgatcgct ccaaggacat gattaaagcc agcggcttta
       61 acatctttcc gcgtgaagtt gaagaagttt tactgcttca tcctgccgtg caggaagcag
      121 cagtgatagg cgttcctcac gagtatcggg gcgaaacagt ggccgccgtc atcgtcttga
      181 aacctggttt tgcaccatcg aaagacactc gcgaggatat tctcgcgtac tgcaagaaag
      241 aattaacccc gtacaaagtg cccaagttca tcgagttccg tgaaagtctt ccaaagacgc
      301 tcataggcaa agtattaaaa cgggaactca gagaaacatt caaaccgtcg taatgtaaat
      361 gtgtagccgg gtaggggcgg gggtagtgtg gagtggggag gggacgcttg cgtcgccccc
      421 ttggctgaac cagatgcagg gagaaaaagt cgcatatgtc aacaaccatg aaacccacag
      481 aggctggtac cgccttcctc acgacacccg taagcgaaaa cgccgacaaa atctttactt
      541 tagagcagcg cgacgaagag cagcgctgga ttgcagagtc agccgccacc ttcgcgcagc
      601 gcgaaatctt gccaaacgta gaagccatcg atcaccagga acctggcttg atgccgagcc
      661 tgttgaagaa agcaggcgag cagggtctct taagcatcga cgtgccagaa gcttacggag
      721 gagccgagct cggcctcttg actagcgccc tggtcgcctc agagttccgc gagggttcat
      781 ttggcaccgc cgtgggtgcg catacaacca ttgggacgct tcctatcgtc ttttacggaa
      841 ccgaggccca aaagcaattc tatctgccga agctggctac gggtgaatgg atctctgcct
      901 atgcgctgac cgaaccaggg gttggttccg atgcgatggg attgaacacc cgcgcggagc
      961 tttctgagga tggtaaatac tacatcctga acggcacaaa gcagtggatc tcgaacgcag
     1021 gattcgccga cgtaatgatt gtgtttgcgc gtatcggcga tgcgagaccc tcggctttca
     1081 tcgtcgagac aagctggccg gggatttcta cgggcgcgga agagcgcaag atgggtatca
     1141 aaggctcttc tacgcgccag gtctactttg agaatgccaa agttcctgtt gaaaatatgc
     1201 tgggtgagat tcacaaaggg tataagattg ctttcaacat cctcaatatt ggacggctga
     1261 aactgggcgc gggtgccgcg gttggttcac gtaatgctct gaaccttgct gccgcatata
     1321 ctaccgagcg taaagctttt ggcaagttcc tgcacgagtt tggcttgatt caaaagaaac
     1381 tggcgcgtat ggccgctgaa acctatgccg ctgaaagcga agcgttccgc accgctgcca
     1441 atatcgaaca ggctcgtcgc agcgctggcg acaacactga agccgctttt aaagcaatcg
     1501 aagagtatgc tatcgaagcc tcgattgcca aggtgcatgg cagcgaagta ctggcatatg
     1561 tcgtcgacga gggcgtgcag atctttggtg gatacggctt catgcaggaa tatcccatag
     1621 agaaggccta tcgcgatgcg cgtattcagc gtatctacga gggcacgaat gagattaatc
     1681 gcctggtcac ctccggcacc ttgttccgcc gcgtgatgtc tggcaaactc gacctgctgt
     1741 cgcgcattcc tgtcattgaa gcgcgtgtca agtcgggcca ggctcctgac gttgctggcg
     1801 aagccattcc cgctgaattg cgctcagccg tgaatgctct ggagcgggcc aaggatgcga
     1861 caatctatgc cgcaatgcgt atcgcgatga aatatatgca ggcgctcgag caggagcaag
     1921 aattcattga atacctggcc aacttgttaa tcgacctata cgccatcgat agcgcgctcg
     1981 cccgcgccac ccaggctgtg cgccggggtg atgcgaacag cgccacgcac gtcaaactgg
     2041 cgcagctcgc aacctggtta gccttctctc gcatgcgcct aaacctggat cagatgatta
     2101 tgaccaatat gaacgaagac caggtcgaaa aagagcttgc ccgcgtccgg gcctatctcg
     2161 gtgattacct gataaacggc gtcaccatgc agcgcgaact tgccgcaatc gtagtcgaaa
     2221 aacaggggta tcctctataa ggagaaacaa acacatgtcc gaagccgtca tcgttagtgc
     2281 cgtgcgcaca cccattgggc gcgccaacaa aggcgtactc aaagatgtgc gcgccgatga
     2341 tcttgccgcc tttgcagtta aggccgccgt agatcgcgta ccacaacttg atcgttcgct
     2401 tattgaagat gttatccttg gctgcgcttt tcccgagggc gaacagggca tgaacctggc
     2461 gcgcctggtg ggagcgctcg cgggattgcc ggaaacagca ggtggcgtga ccgtcaatcg
     2521 cttctgcgcc tccagcttgc aagccg
//
LOCUS       DASZAK010000113         7763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_179757, whole genome shotgun sequence.
ACCESSION   DASZAK010000113 DASZAK010000000
VERSION     DASZAK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7763
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179757"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VGT44_01910"
     CDS             complement(<1..252)
                     /locus_tag="VGT44_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579580.1"
                     /translation="MILPLLAGLLVATIVSHDAALELQLTLSSKYRFTALLRLCLVAG
                     WVACVALISNVLIYHLKFWRIPAQVSTWQVLPQVLIGQFT"
     gene            691..1683
                     /gene="glk"
                     /locus_tag="VGT44_01915"
     CDS             691..1683
                     /gene="glk"
                     /locus_tag="VGT44_01915"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEV2579581.1"
                     /translation="MLLAGDIGGTKTNLGLFTHDGDWHDPVHEATFPSGNYPSLEALV
                     QEYFAQQKVTSIDHASFGVAGPVLEGHATITNLPWKMDERHLQQELNIPSVSLLNDLE
                     AIAHAVPFLDERDLATINRGQAESKGALAVIAPGTGLGEAFLTWNGTAYQSHTSEGGH
                     ADFAATNAFELDLLRYLLDHFPHVSYERVCSGKGFPNIYAFLKERGLAEEPDWLAREL
                     AQAQDKTPVIMQAALQDACDIAVKTLNVFVAVLGAEAGNMALKVLATGGVYLGGGIPP
                     RILSYLSDELFMKAYTNKGRFGKLLSTIPVHVILNPKVALLGAARHGFEKIKDD"
     gene            1686..1991
                     /locus_tag="VGT44_01920"
     CDS             1686..1991
                     /locus_tag="VGT44_01920"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HEV2579582.1"
                     /translation="MIRKQFVNSRKGKVARVTFSLPGSAWADAIYLVGDFNNWNETSH
                     PFSIGRDGDWSITVDLETGREYQFRYRKDGEWMNDSHADGYSSNPYGSDNFIVNTRD"
     gene            complement(2443..3030)
                     /locus_tag="VGT44_01925"
     CDS             complement(2443..3030)
                     /locus_tag="VGT44_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2579583.1"
                     /translation="MNEPTSVNRPMRADAQRNYASLLNAARAAVAERGADIVLEDIAR
                     SAGVAIGTLYRHFPTRQDLLEAVFLDETNELREHAEALASAPVPFDALMSWLHLQMDF
                     ATRGRSMGAAILAAKHVAGTRISAANKAMHEAGEVLLLRAQAAEQIRTDVHMLDVIRL
                     VYGIALVNEHASDPDGANRMLDLVIAGIRTKPSRD"
     gene            3192..4031
                     /locus_tag="VGT44_01930"
     CDS             3192..4031
                     /locus_tag="VGT44_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2579584.1"
                     /translation="MRVFVTGATGFIGSVVVRELIGAGHTVVGLTRSDKGAEALSEAG
                     AEVHHGTLDNLDSLRLGAAAADGVIHLAFQHDSSDFAAALTTDLRAVETMGTALEGTG
                     KPFVTTAHLNGEASDNAALALAGVRSSIVSLSLSVHDEDDRHGFIPRLITLARAKGIS
                     AYIGDGFNRWPAVHRLDAACLYRLALESAPAGSRLDGVGDEGVPFRDIASVIGRHLNL
                     PVVSISREEAEVHFGSFLGTVAAVDRPRSSARTQELLGWRPVHPGLMMDLEEGHYFDS
                     SVA"
     gene            complement(4109..5665)
                     /locus_tag="VGT44_01935"
     CDS             complement(4109..5665)
                     /locus_tag="VGT44_01935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2579585.1"
                     /translation="MGAIRELRLRLSRLSLQTKLIVSYLGVALGAILLVIVISVLVQN
                     YFNSAQRDELRSQAEYLAQKVGIIYFNHGEDWTNVGSIAYYNSQLFVVLDANQQVHST
                     EPPGIPRLSDGDIFTLNTAMLQSLQGREVSGTFQGIGNDQDGDVFTGNFISVPIYDNG
                     QQSGKPVGALLLAQPDRYPTGFSPKDVLATLNTLLIVAGVAITGIVLLFGIFMARRFT
                     RPLASLTHAAERMKAGDYSQRVDTLDRQDEIGALSLTFNAMADKIESDVNELRLQEQL
                     RRDMIANIAHDLVTPLTAIQGFSEAMADEVIYDPQARQETAQLIGREVQRLRRLVSDM
                     QHMTTLESGRVQLELAPLDMRALVDEVLAVIGPECEQAGITLRNAIAPGAPKTLADSD
                     RITQVLLNLLDNARRHTPAGGSITIDASVIADTPGGPGISGRKWLEVAVHDSGSGINA
                     ADLPFIFDRFFRSDRARTGQKRGSGLGLSIVKAIITAHGGTIRAESAPGQGTRIVFTL
                     PVADGKFPTK"
     gene            complement(5673..6464)
                     /locus_tag="VGT44_01940"
     CDS             complement(5673..6464)
                     /locus_tag="VGT44_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2579586.1"
                     /translation="MTKILVIEDDNNIAQILRLYLEQANFTVVTASDGAAGLELHARE
                     HPDLVILDLMLPALDGMEVCRRIRAWASTPILMLTARQSEEDRIAGLELGADDYLAKP
                     FSPREVVSRVKAILRRSTPSFAPSSETGMSTDQGSMTTAAALVMPGAFSDQETLSREK
                     EELRFGGLHITIPARRVEVNGQNVTLTAKEFDLLTTLASSPDRVFTRETLLDKIWGYT
                     FLGDGRTVDVHVGTLRKKLEAASEPGSVSPIKTVWGVGYKFAPEE"
     gene            6739..>7763
                     /locus_tag="VGT44_01945"
     CDS             6739..>7763
                     /locus_tag="VGT44_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2579587.1"
                     /translation="MMNNPYLDDENAPTPQPANIYQKYPYYRQEQMGPMGPVDQAGQT
                     PPSIPLVQPAYTTRPTPGQFQQTPMPPPPVARSGRGLRTGSIIALTALLAVIFGVGLF
                     AGWQFGRTSVATLPSSGTSALQPGNTPVGNIPALTGNNLQAVREAVIAKVRPAVVEVN
                     VTTTSGGAIGSGVIIDGRGYIVTNNHVVVGAQGIEVVLFDGTHLTAQVAGTDPTDDLA
                     VIKVTPPKNGLTTLKLGNSSQLQVGEDVLAVGNPLGITQTVTSGIISALGRSVSEGQG
                     GATIPDAIQTDAPINPGNSGGALVDMQGNLIGIPTLTAIDPEFNTPASGVGFAIPSNR
                     VGFIVPQ"
BASE COUNT         1681 a         2220 c         2217 g         1645 t
ORIGIN      
        1 tgtgaactgt cctatgagaa cttgtggcag tacctgccac gtggaaacct gggccgggat
       61 gcgccagaat ttaaggtggt aaatcaaaac gttggaaatc agcgccacgc acgctaccca
      121 ccccgcgacc aggcataaac gcagaagagc ggtgaaacga tacttgctgg acaacgtcaa
      181 ttgcaactca agcgcggcgt cgtggctgac gatggttgcc accagcaaac ccgctagaag
      241 cggcagaatc atttcgagac tcgctgttag cacctgagcg attctgagat gatccacctg
      301 cattaccgat aatatcaccg ccagtgctgc aaagcagctc atgcacagca agggtagaag
      361 tatcaccaat tttccggcaa ttcgtaattc gtaccgaatc ctatcaagaa acatacgtta
      421 cctcgagttt gatgttttgc tgtttcgcct gaggtattat gacctggcag gcgaatcctt
      481 atgtctactg tcaactatag tggaaagatg tcgctaccag atcatgcatt tgtcgcaaag
      541 ttgtcgcagc atcctacgct gtaccattgc ttgctgattg gagacaacag gagaagagta
      601 ctggattagc ctgtgttgat gaggccaaac gtcgcaaaag ataatacaat agagcaaaga
      661 atgctataac gatcaaagga gaacatggat atgctactgg ccggagatat tggcggcacc
      721 aaaacaaatc ttgggctttt tacccatgat ggagactggc atgatccagt tcacgaggca
      781 accttcccca gcggaaacta ccccagtctt gaggcgctgg tgcaggaata ttttgcgcag
      841 caaaaagtta catcaatcga ccatgccagt tttggcgtcg ccgggccggt cctcgaagga
      901 cacgctacca tcacaaactt gccctggaag atggatgagc gtcatttgca acaggaactc
      961 aacattccat cggtgagcct gcttaatgat ctggaagcca ttgcccatgc cgtccctttc
     1021 ctggatgagc gcgacctggc aaccatcaat cgaggccagg cggagtccaa aggcgcgctt
     1081 gctgtgattg cgcccggcac cggcctgggc gaggcgtttc tcacctggaa cgggacagcc
     1141 taccagtccc atacctccga gggcggccat gccgattttg ctgcaaccaa tgccttcgag
     1201 ttggacctgc tgcgttacct gctggatcat ttcccgcatg tcagctacga gcgcgtctgc
     1261 tccggcaagg gcttcccaaa tatttacgca tttttgaaag agcgcggcct cgctgaggag
     1321 ccagactggt tggcgcgtga actggcccag gcgcaggata aaacgccggt gataatgcag
     1381 gcggctctac aagatgcgtg cgatatcgcg gtcaagacgc tcaatgtctt tgttgccgtg
     1441 ctgggcgcgg aggccggcaa catggcgctg aaggtcctgg cgacgggagg cgtctacctg
     1501 ggaggcggca ttccgccacg cattctctct tatctatcgg acgaactctt tatgaaggcg
     1561 tataccaaca aggggcgctt tggtaaactc ctctcaacca ttccggtgca tgttatactc
     1621 aatcctaaag ttgccctact gggtgcagcg cggcacggtt ttgaaaaaat caaggatgat
     1681 tagtcatgat tcgtaagcaa tttgtcaact cgcgaaaagg caaggtggcg cgcgtcacct
     1741 tcagcctgcc tggcagcgcc tgggccgatg ccatctacct cgtcggcgat ttcaacaact
     1801 ggaacgagac ctcgcatccc ttctcgatcg gtcgtgacgg ggattggagc atcaccgtag
     1861 acctggaaac gggccgcgaa taccagttcc gctaccgcaa agacggcgag tggatgaacg
     1921 atagccacgc cgacggctat tcgtcgaacc cgtatggcag cgataatttt atcgttaata
     1981 caagggattg acgtttaaac taaaataacc tccgcaatca ttaagagggg attgccaagg
     2041 tggaggaata tatccaaaga gtaaattggg tgcgcatcag cacaactttt gaggcgaaac
     2101 ggacaacatc agcgcgaaac agggaaattg actctggaga aacgagagaa ggagcctcag
     2161 aaacgaaggg ttcgcaccat tttgggaaat gggtgagggc agcgcagggg acatcttgtg
     2221 gttacaacag gtgctggatg tttcccaccc attaccacct cctttgcggt ctgtgtcagc
     2281 ttcgctggta ctgggcctcc atgcccgttg agagaacgtt ccaaggttgg ccctccccca
     2341 agattgcctt tcgactttcc agcgaagccg gtgtgcatgc ttaactggcg cacaacgtcg
     2401 tcaataggat cgactcctcg ccgagaggtg acacaccatc ttttagtccc gactcggctt
     2461 tgttctgatt ccggcgatga cgagatcgag catgcggttg gccccatcag ggtcggaggc
     2521 atgttcattc accagggcga ttccgtagac gagtcgtatc acgtcgagca tatggacatc
     2581 tgttcggatt tgttctgcgg cctgggcgcg gagcaagagg acttctcccg cctcgtgcat
     2641 cgctttgttc gcagcagaga ttctcgttcc cgcaacgtgc ttggcagcca ggatggcggc
     2701 tcccatgctc cgccctcgtg tcgcgaagtc catctggaga tgaagccagc tcatcaaggc
     2761 gtcgaaagga acgggagcgc tggcgagtgc ttcagcatgc tccctcaatt cattggtctc
     2821 atccaggaac acggcttcaa ggagatcctg acgtgtggga aagtgacggt acagcgttcc
     2881 gatggcgacc ccagccgacc tggcaatgtc ctcgagaacg atgtcagcgc cgcgctcggc
     2941 gaccgcggct cgcgctgcat tcagcaggct ggcgtagttg cgctgagcat cagctcgcat
     3001 tgggcggttg actgatgttg gctcgttcat tggatatgac tcctttctcc cagggagatc
     3061 aacccttgac aaagtgaggg ttcctccgtt attatagaga ggtaacatga gtgtccctcc
     3121 gtttatatag ggaggtagcc tcattttatc acacttatgc ttgatcttac cagaagaagg
     3181 agaactttct tatgcgtgtt ttcgtcacag gagcaacagg cttcatcggt tccgttgtcg
     3241 tccgcgaact catcggtgcc ggtcatacgg tcgtcggtct tacccgctca gacaaggggg
     3301 ccgaagctct ctcagaagcc ggggccgagg tgcatcacgg tacccttgac aacctcgaca
     3361 gcctgcgcct cggtgccgcc gcagcggatg gcgtcattca cctggcgttt cagcacgact
     3421 cttcggactt cgcagccgcg ctcaccaccg atctgcgcgc cgtcgagacg atggggacgg
     3481 cgctcgaagg cacaggcaag ccgttcgtga ccaccgctca cctcaacggg gaggcatcgg
     3541 ataacgcggc gctcgcactg gctggagtgc gatcatcgat tgtctctctc tctctgtccg
     3601 tgcacgacga ggatgacagg cacggcttca taccgagatt gatcaccctc gctcgcgcga
     3661 agggtatctc tgcctatatc ggcgacgggt tcaaccgctg gcccgccgtc catcgcctcg
     3721 atgcggcgtg cctgtaccgc ctggcactgg aaagcgcccc ggcggggtca cggcttgatg
     3781 gggtcggcga cgagggcgtg ccattccgcg acatcgctag cgtcatcggt cgccacctga
     3841 acctgccggt ggtcagcatt tcccgcgaag aggcagaggt ccacttcggc tcttttctcg
     3901 gcaccgtggc ggcggtcgac cgcccgaggt cgagtgcacg gacccaggaa ctcctgggat
     3961 ggcggcctgt gcaccctgga ctgatgatgg atctggagga gggtcactac tttgacagct
     4021 cagtcgcata gtcagcaacc agacgacacc gggtcggcga gtatgagtaa gctcaacgtt
     4081 tgaatgaact taagagggtg cactcatctc actttgtagg gaatttacca tcggccaccg
     4141 gcagcgtaaa cacaatcctc gtcccctggc cgggcgcgct ttcagcgcga atcgtgccac
     4201 cgtgcgcggt aatgatggct ttgacgatgg agaggccgag gccgctgccg cgcttctggc
     4261 cggtgcgggc gcgatcggag cggaagaagc ggtcgaagat gaagggcaga tcggcagcgt
     4321 tgatgccact gccgctatcg tggacagcca cctccagcca cttgcgccct gatatccctg
     4381 gcccacctgg cgtatccgcg atgacactcg cgtcgatggt gatcgagccg cctgcgggcg
     4441 tatgacggcg ggcattgtcg agcaggttga gcaagacctg ggtgatgcgg tcgctatccg
     4501 ccagcgtttt cggtgcgcct ggcgcgatgg cgttgcgcag cgtgataccc gcctgctcgc
     4561 actccgggcc aatcacggcc agcacctcgt cgaccagcgc gcgcatgtcg agcggggcaa
     4621 gttcgagttg cacccgcccc gattcgagcg tggtcatatg ctgcatatcg cttaccaggc
     4681 ggcgcaggcg ctgcacctcg cgcccgatca actgcgcggt ctcctggcgc gcctgcgggt
     4741 cgtagatcac ctcgtcggcc atcgcttcgc tgaagccctg gatggcggtc agcggcgtca
     4801 ccaggtcgtg ggcgatattg gcgatcatat cgcggcgcag ttgttcctgc aagcgcagtt
     4861 cattcacgtc ggattcgatc ttgtcggcca tggcgttgaa ggtcagcgac aacgcgccga
     4921 tttcatcctg cctgtcaagc gtatcgacgc gctgcgagta atctcctgcc ttcatgcgct
     4981 cggctgcgtg tgtcaaagat gcgagcgggc gcgtgaagcg gcgcgccata aagatgccga
     5041 acaggagtac aatgccggtg attgctaccc ctgccacgat caacagggta ttcagggtgg
     5101 ccagcacgtc cttaggcgaa aaccccgttg gatagcggtc cggttgggct agcagcaatg
     5161 ctccaaccgg tttgccgctc tgctggccgt tgtcgtagat cggaacgctg atgaagtttc
     5221 cggtgaaaac atccccgtcc tggtcgttgc ctatgccctg aaaagtgccg gatacctccc
     5281 gcccttgcag tgactggagc atcgcagtat tcaaggtgaa gatatctccg tcgctcaatc
     5341 tcggaatacc gggtggttct gtgctatgga cttgctgatt ggcatcgagt acgacaaaga
     5401 gctgtgaatt atagtaggcg atacttccaa cattggtcca atcttcgccg tgattgaaat
     5461 agatgatccc cactttttgc gccaggtact ccgcctgcga gcgcagttca tcgcgctgag
     5521 cgctgttgaa gtagttctgc accagcaccg aaatcacgat gactagcagg attgcgccta
     5581 gcgcgacacc caggtagctg acgatcagct tggtctggag cgagaggcgc gacaaacgca
     5641 ggcgcaattc ccgtatagca cccataagcg acttactcct ctggcgcgaa tttgtagcca
     5701 acgccccaca cggttttgat tggcgagacg cttcccggct cgcttgccgc ctcaagcttc
     5761 ttgcgcagcg ttccgacgtg cacatcgacc gtgcgcccat cgcctaagaa ggtgtagccc
     5821 cagattttat ccagcagcgt ttcgcgtgtg aagacgcggt cgggcgagga ggccagtgtg
     5881 gtgagcaggt caaattcttt cgcggtcagg gtgacattct ggccgttgac ctctacgcgc
     5941 cgggcgggta tcgtaatgtg cagcccgccg aaacgcagtt cctctttctc cctactcagc
     6001 gtttcctggt cggaaaaggc gcccggcatc accagcgcag cggcagtcgt catgcttccc
     6061 tgatccgtgc tcatgccagt ctcagaggat ggcgcaaacg aaggagtcga acggcgcaaa
     6121 atggccttga cgcggctcac cacctcgcgc gggctaaacg gcttggccag gtaatcgtcc
     6181 gcgccgagtt ccagcccggc gatgcggtct tcttcggatt ggcgcgcagt cagcatcaga
     6241 atgggtgtac tggcccaggc gcggatacgg cggcagacct ccatgccgtc cagcgcgggt
     6301 aacatcagat cgaggatcac caggtcggga tgctcgcgcg cgtgaagttc cagccccgcc
     6361 gctccatcgc tggcggtgac aacggtgaag ttagcctgtt ccaggtagag gcgcagaatc
     6421 tgcgcaatat tattatcgtc ttcgatcacc agtatctttg tcatcgttat tcaatcctca
     6481 taacatatca tactctacta actgtacaga aaaaagatca gaaagagcgc ggatatttgt
     6541 aaaattactg taagactctc tcatgccatt ccaatcttcc attatatgag acttgtggat
     6601 agcttacaat cattttacag tttcctgtga attttctcat atctgtgcgc tatagtaaca
     6661 acagatgcat gtgagcgagc tatattccga gacagacagg acaatcgtga taaataaaaa
     6721 catagtggaa ggacagatat gatgaataat ccctacctcg atgacgagaa cgcgccaacg
     6781 ccgcaaccgg cgaatatata tcaaaagtac ccctattatc gccaggagca aatggggcct
     6841 atgggaccgg ttgaccaggc cgggcaaacg ccgccatcga tcccgcttgt tcaaccagct
     6901 tatacaaccc gtccgacacc gggacagttt caacagactc ccatgccccc cccgcctgtc
     6961 gcgcgcagcg gacgcggact gcgcaccggc tcgatcattg cgctgaccgc gctattggcc
     7021 gtgatcttcg gtgttggcct gttcgcgggc tggcaattcg gcaggaccag cgtcgctacg
     7081 ttgccatcaa gcggcactag cgcattacag ccgggcaata cgccggttgg aaacataccg
     7141 gctctgaccg gcaataatct ccaggcggtg cgtgaagcag tgatagcaaa agtgcgcccg
     7201 gctgtcgtgg aagtgaacgt gaccaccacc agcgggggcg cgattggctc tggcgtaatt
     7261 atcgatggcc ggggctatat cgtcaccaac aatcacgttg ttgttggcgc gcagggcatc
     7321 gaggtagtgc tgttcgatgg cacccacctg accgcgcaag tggcgggaac cgacccgacc
     7381 gatgacctgg cagtcatcaa agtgacgccg ccgaagaacg gcctgaccac attgaaactc
     7441 ggcaactcat ctcagttgca ggtcggcgaa gatgtgctgg ccgtcggcaa tcccctgggt
     7501 atcacgcaga cggtcacgag cggcatcatc agcgcgcttg gccgcagcgt ctccgaagga
     7561 cagggcggcg cgaccatccc ggatgcaatc cagaccgatg cgcccatcaa tcccggcaat
     7621 agcggcggcg cgctggtaga catgcagggc aacctgatcg gcattccaac cctgaccgcc
     7681 atcgaccctg agttcaacac gccggccagc ggtgtcggct tcgccatccc gtccaatcgc
     7741 gtcggcttta tcgtgccgca gat
//
LOCUS       DASZAK010000114         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_570849, whole genome shotgun sequence.
ACCESSION   DASZAK010000114 DASZAK010000000
VERSION     DASZAK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570849"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..2893
                     /locus_tag="VGT44_01950"
     CDS             47..2893
                     /locus_tag="VGT44_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEV2579588.1"
                     /translation="MSEEETQDQDVQRYMRPMQGALTVAEGTGGPITCLGLSFASDSE
                     RRAYFTERLREKLADPTFRTQEGFPIGSDEDILALSDPPYYTACPNPFLADFERCFNT
                     PYDASVPYRREPLAVDVSEGRTDSIYTAHSYHTKVPHKAIMRYIMHYTQPGDIVLDGF
                     CGSGMTGVAAQACANPEGEYKIQIESEWTQAGRGLPEWGRRYSILNDLSPSATFIAYN
                     YNTSVDGKLFEQAANALLSRFEATYGWMYKTGHSDGKCEGRINYVIWSDVFVCAVCNR
                     EIIFWDAAVHNGEVQDTTTCPHCDAKMSKDALDRASIAIFDPTLNQTISKAKRVPVLI
                     NYTVGGKRFEKRPDSADLELLDRIEREPIPGWYPTRRIDEDIDLWYERDYRSLGIFSI
                     DAFFFRRNMIMVSYFKAEIEKTGGRLQGFLWFWFQSVLMGFSLLNRYRAKGFSQVNQL
                     LSGTLYIGAILAEVSPWYALTGKISRLQAIARMADSGVIVSTSSTTQMAIPDDSIDYI
                     FTDPPFGSNIIYSDLSILWESWLRISTNTDQEAVVHRRKRRGASRLADYQQLMTSCFA
                     EMHRVLKPGRWITIEFHNTKNAVWAAIQESLLHAGFVIADVRTLDKQMGTFKQVTTAS
                     AVKQDLVISAYKPTGELKDRFKLDAGTEEGVWDFMRTHLRQLPVFIPTQDGRAEVIAE
                     RLDYLLFDRMVAFHVQRGVAVPLSAPEFYAGLRQRFPQRDEMFFLEEQVAEYERKRLS
                     VREIQQLTIFVKDESTAIQWLRQQLANKPQTQAELTPQFMRELGAWDRHEKLPEMIEL
                     LEQNFLRYDGEGPVPAQIVGWMRKSSELRELIEQKIAAGMLRADGGLDTRESRLLTQA
                     RDRWYVPDPYKAIDLEKLRTRALLREFATYAEGRGKLKLFRTEAVRAGFAAAWRERDY
                     LTITRIAERLPESVLQEDPDLLMYYDTASLHVRP"
BASE COUNT          736 a          786 c          826 g          665 t
ORIGIN      
        1 atgcgcaggc ggcagcagtc acagcacagt tgagcggagg gagtgcatga gcgaggaaga
       61 aacgcaggat caggatgtgc agcgatatat gcggccaatg cagggggcat tgacagttgc
      121 tgaggggact ggaggtccca ttacatgctt aggtctttct tttgccagcg attcggaacg
      181 gcgagcctat ttcactgagc ggctgcgcga gaagctggcc gatcccacct ttcgcacaca
      241 ggaaggtttt ccgataggga gcgatgaaga tattctagcc ctctccgacc cgccgtacta
      301 cacagcctgc ccgaacccgt tcctcgcgga cttcgagcgc tgcttcaata caccgtatga
      361 tgcgagcgtg ccctaccgcc gcgagccact agcggtggat gtcagcgaag ggagaacaga
      421 ttcgatctat acggcgcact cctatcacac caaagtgccg cataaagcta ttatgcgata
      481 tatcatgcat tacacgcagc caggtgatat agtactcgac ggattctgcg gttcaggcat
      541 gacgggcgta gctgcacaag catgtgcgaa tccagaggga gaatacaaaa tccaaatcga
      601 aagtgaatgg acgcaggcag gccgaggctt gccagaatgg ggacgcagat actcaatctt
      661 aaacgatctc tcgccctcgg ctacctttat cgcctacaac tacaacacgt ctgtcgacgg
      721 taagctattc gagcaagctg ctaatgctct tctgtcaaga tttgaagcga catacggatg
      781 gatgtacaag actggacatt cagacggcaa gtgcgagggt cgaataaact atgttatatg
      841 gtcggatgtg ttcgtttgcg ctgtatgtaa ccgtgaaatt attttttggg atgctgctgt
      901 ccataacggc gaagttcaag atacaaccac ttgtccacat tgcgacgcta aaatgagcaa
      961 agacgcccta gatcgtgcct ctatagcgat atttgatcct acactcaatc aaaccatttc
     1021 aaaggccaaa cgcgttccag tgcttattaa ttacacagtg ggcggtaagc gatttgaaaa
     1081 gaggccggat tcagcggact tggaattgct ggatcgcatc gagagggagc caataccggg
     1141 ctggtacccc acacgcagga tagacgagga tattgatctt tggtacgaac gagactaccg
     1201 aagtctcggt atcttcagta tagatgcttt cttcttccgg cgaaatatga ttatggtgtc
     1261 ctatttcaaa gctgagattg agaagacagg gggcagatta caaggctttc tatggttttg
     1321 gtttcaatct gttcttatgg gattctcact gctcaaccgc tatcgagcta aggggttctc
     1381 ccaggtcaat caactgctta gtggcacact atatatcggc gctatacttg cggaagtcag
     1441 cccgtggtat gcattgactg gcaaaatcag ccgtttacaa gctatcgcac gcatggctga
     1501 ctcaggtgtt attgtttcta cgtcttctac aacgcaaatg gcgattccgg acgatagtat
     1561 cgattacatt ttcacagatc ccccgttcgg tagcaatatc atttactcgg accttagcat
     1621 actctgggag tcttggctcc gaatctcgac caatacagat caagaagctg ttgtacatcg
     1681 ccgaaaaaga aggggagcct ccaggctcgc ggactatcag caacttatga cctcgtgttt
     1741 tgctgagatg catcgagtac taaagcctgg acgatggatt accattgaat tccacaacac
     1801 caagaacgca gtatgggcag cgattcaaga atcgcttttg catgcaggat ttgtcatcgc
     1861 tgatgtccgg acgttggaca agcagatggg cacatttaaa caggttacca ctgccagtgc
     1921 cgtcaaacag gacctcgtga tctccgccta taagccgact ggcgagctga aagaccgctt
     1981 caaactggat gctggcactg aagagggcgt ttgggacttt atgcgcacgc atcttcggca
     2041 actccctgtg ttcatcccaa cgcaggatgg ccgtgccgaa gtgattgccg agcggctcga
     2101 ctacctgctc ttcgaccgca tggtggcttt tcatgtccag cgaggcgtgg cggtgccgct
     2161 ctccgcgccg gagttttacg ccggactacg ccagcgcttc ccgcagcgcg atgaaatgtt
     2221 cttccttgag gaacaggtcg ctgagtatga gcgtaagcgg ctctccgtac gtgagattca
     2281 gcagctgacc atcttcgtca aggacgagtc gaccgcgatc cagtggctgc gccagcagtt
     2341 agcaaacaaa ccccagaccc aggctgaact gacgccacag ttcatgcgtg agctgggtgc
     2401 ctgggacagg cacgagaaat tgccggagat gatcgaactg ctggagcaga acttcctgcg
     2461 ctatgatggc gaggggccgg tccccgcgca gatcgttgga tggatgcgta agagttcaga
     2521 gctgcgagaa ctgatcgagc agaagatagc cgcgggaatg ctgcgggctg atggcggcct
     2581 cgatacacgt gagagcaggt tgcttaccca ggcacgcgat cgctggtacg tacccgaccc
     2641 ctacaaggcc atcgacctgg agaagctgcg caccagggcg ctcttacgcg aattcgcgac
     2701 ctatgccgag gggcgcggca agctcaagct ctttcgcacc gaggcagttc gggcgggttt
     2761 tgcggcggca tggcgcgaac gggactacct gaccatcacg aggatagccg agcggctccc
     2821 cgagtcggtc ctacaggagg atcccgacct gctcatgtac tatgacacag ccagcctgca
     2881 cgtgcgtcct tagcaagtga gccagagaag accatgagcg aatccgtact cgagacaatc
     2941 ctggaactgt tcccacctca cacgcacccc ctcacgcttg tcagtgatcc agatgaactg
     3001 ctggcagatg cag
//
LOCUS       DASZAK010000115         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_499792, whole genome shotgun sequence.
ACCESSION   DASZAK010000115 DASZAK010000000
VERSION     DASZAK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499792"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(564..830)
                     /locus_tag="VGT44_01955"
     CDS             complement(564..830)
                     /locus_tag="VGT44_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6582 domain-containing protein"
                     /protein_id="HEV2579589.1"
                     /translation="MSKLSHNDRENIPDSDYAFPKERKEPLSDARHVRDAIARFDQVE
                     GVTNKERDEAWKRIVRAAHKFDVELQEQNWRDLFKRNGRAIPKD"
     gene            complement(847..1617)
                     /locus_tag="VGT44_01960"
     CDS             complement(847..1617)
                     /locus_tag="VGT44_01960"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV2579590.1"
                     /translation="MGILEKQVAIVTGADSGIGRAIAIQFARAGATMVVNYAHNQQKA
                     EEVRQIIQQTNGNVLVVQADIGQYQQAIGLIQQTVEHFHQLDILVNDAGMEIHSAFVD
                     VTEEMFDRVLSIDLKGAFFCAQAAARAMIQRQNAGRIINISSVHEDLAMPRNVPYCCA
                     KGGLRMLTRTIAIELAPYNITVNNIAPGAINTPIDADVIADPQKMGVLLKEIPLNRMG
                     DPDEVAKLALFLASDEAAYITGSTYLIDGGLTVNTGAL"
     gene            complement(1623..1889)
                     /locus_tag="VGT44_01965"
     CDS             complement(1623..1889)
                     /locus_tag="VGT44_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579591.1"
                     /translation="MAVYRFCPPNSCPLTEVVKQQSGTRYPHAYNSCVSGDVPPIPPH
                     ITLEQAKAYASALVKGDPDEAGIIKQTVKDVASGIFHRKGAEKD"
     gene            complement(1933..2613)
                     /locus_tag="VGT44_01970"
     CDS             complement(1933..2613)
                     /locus_tag="VGT44_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579592.1"
                     /translation="MKQETSEKPEQTTQVAPTRKEMASRWAALVGMVAVGVIYAALPS
                     TLVIGPSWILLAIEAVFIIPIAIYLISGRELSHTTIRAVLLLLLAIITLALVIGVLLL
                     IFNLPTDKNAVNLLRTAALLWGFNVLVFGLWYWEIDGGGPYKRHQSGHQATDFMFPQQ
                     VDSNKSGWVPHFLDYLFLAFTGATALSPADTYPLTRTAKALMMIEAVLSMTIIVLLAA
                     RAVNILGS"
     gene            complement(2639..>3132)
                     /locus_tag="VGT44_01975"
     CDS             complement(2639..>3132)
                     /locus_tag="VGT44_01975"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2579593.1"
                     /translation="MQFIFAPMQAGDALSIAAWRYEEPYTIYNTEDGDDMTEMLDRRS
                     PYYAVRDERGELIGFFAYGSAAYVWGSDEPHLYSQGNTIAVGLGMRPDLTGRGLGLAF
                     VQAGMDFARKHYAPERFRLYVLTFNERAIRVYERAGFERAEIYVQHTPGGDRDFLEMK
                     RKV"
BASE COUNT          706 a          849 c          834 g          743 t
ORIGIN      
        1 cgcgacggta aacgagacat catcgagtac gaggcgtgga ccgtaagaaa gatgtatatg
       61 attgacttgt aacatgtgta aatttccttt tgtaactctc caggtatgct ctgcatacca
      121 ccagtctgct cttagcctct cgtgtaacaa tgcgtgaaag tctgaacatg cgtaccggac
      181 aaggcgcaat gggatgggca tacaatcacg cgaatggatg tgttgggaac cacagcatgt
      241 tgcgggacag gcagggacag gagaggctcg gtgtccggca acatacgcca ttgtcatcga
      301 gggaggagag gtacgttgga gatgcaaaca aaatggaaaa ctaccactca cctgctaagg
      361 atgagcagag gcatcaagca gcgccacttt ctcccgcgta agcccggcgt gatcgcctac
      421 gcgggtgagc gggacatttg tatttttact gtaatgaata ttttctgcat ctgtttcgta
      481 ttttctaacc atgttgaaca ggtggttgca ctctgttgag gagatactac cacctggacg
      541 ctttgctgtc aagcaaaagt tggtcagtct tttggtatgg cgcgaccatt gcgcttgaat
      601 aagtcgcgcc aattctgctc ttgcagctcg acgtcgaatt tatgcgcggc gcgcacgatg
      661 cgcttccagg cttcatcgcg ctctttatta gtgactcctt ccacctggtc gaagcgcgcg
      721 atggcatcac gcacgtggcg cgcatcgcta agcggctctt tgcgctcctt cgggaaggca
      781 tagtcactat ccgggatgtt ttctcggtcg ttgtgagaca atttcgacat gctgtttctc
      841 cttgctttat aaggcgcccg tattgactgt cagaccgcca tcgatcaagt aggtcgaacc
      901 agtgatatac gctgcttcgt ctgaggccag gaacagggcc aactttgcca cttcatcagg
      961 atcgcccatg cggttcagcg ggatttcttt gagcagcaca cccatcttct gaggatctgc
     1021 gataacgtcg gcgtcaatgg gcgtgttgat cgcacccggc gcgatgttgt tgacggtaat
     1081 gttatacggc gccagctcga tggcgatggt gcgcgtcaac atgcgcaggc cgcccttggc
     1141 gcagcaatag ggaacgttgc gcggcatcgc caggtcttca tgcaccgatg agatattgat
     1201 gatacgcccg gcgttctggc gctggatcat tgcgcgcgct gctgcctgcg cgcaaaagaa
     1261 agcgcctttc aagtcgatgc tcaggacgcg atcgaacatc tcttccgtga catccacaaa
     1321 ggcgctatgt atctccatac cggcatcatt caccaggata tcgagttgat ggaagtgttc
     1381 gaccgtttgc tggatcaatc caatagcctg ctggtattgc ccaatatcgg cctgcaccac
     1441 cagcacattc ccattagtct gctgaatgat ctgccgcacc tcttcggctt tctgctgatt
     1501 atgcgcgtag ttgaccacca tcgttgctcc cgcgcgcgcg aactgaatag caattgcgcg
     1561 gccaatgcca ctatccgcgc cggtgacgat cgcgacttgc ttttcaagaa tgcccatcgg
     1621 aatcaatcct tttctgcccc tttgcgatgg aagatgccgc tcgccacatc tttcaccgtc
     1681 tgtttgataa tacccgcttc atccgggtcg cccttgacca gcgcggatgc gtaggctttg
     1741 gcctgttcca gcgtaatatg cggtggaata ggtggcacat cgccggagac gcaactatta
     1801 tatgcatggg ggtatctcgt gccagactgc tgttttacta cttctgttaa gggacacgag
     1861 ttgggagggc agaaacgata cacagccata ctgcgcatag tcgagttatg ggctggcgag
     1921 tgtgagaaag aatcagctgc cgagaatgtt gacggcgcgg gcggcgagca aaacaataat
     1981 tgtcatggat aacactgcct cgatcatcat caaggctttg gcagtgcgcg tcagagggta
     2041 ggtatcggca ggactgagag cggttgcgcc agtgaaggcc agaaagaggt aatcgaggaa
     2101 atgtggtacc cacccgcttt tattgctatc cacttgctgt ggaaacataa aatccgtcgc
     2161 ctggtgtccc gactggtggc gtttatatgg ccctccgcca tcgatctccc aataccacag
     2221 accaaagacc agtacgttaa atccccacag cagagccgcc gtgcgcagca ggttcacggc
     2281 gtttttatcg gtgggcaggt tgaaaatcag gagcaaaacc ccgatcacta aggccaatgt
     2341 aatgatggct agcaacagta ataataccgc gcggatagta gtatgtgaaa gctcgcgtcc
     2401 tgagatcagg tagattgcga ttggaatgat aaacactgct tctatcgcca ggagtatcca
     2461 actcgggcca ataacaagcg ttgaaggaag tgctgcatag atgacgccta ccgctaccat
     2521 gccgaccagc gcagcccagc gtgaagccat ttccttacga gtaggagcta cctgtgtcgt
     2581 ttgctccggt ttctcagaag tttcctgttt catcgcgttg tctctccttt aatccgcatt
     2641 acaccttgcg cttcatctcc aggaaatcgc gatctccgcc gggagtatgc tgcacatata
     2701 tttctgctcg ctcaaagccc gcgcgctcgt agacacggat agcgcgttca ttgaaggtca
     2761 ggacatacag gcggaagcgt tccggcgcat aatgtttccg ggcaaagtcc atgcctgcct
     2821 ggacaaacgc cagccccagc ccgcgacccg tcaggtctgg ccgcatcccc aggccaaccg
     2881 cgatggtatt gccctggctg tataaatgcg gctcatcgct gccccacaca tacgccgccg
     2941 agccatatgc gaaaaagccg atcagctctc cacgctcgtc cctcaccgcg tagtacgggc
     3001 tgcgccggtc gagcatctct gtcatatcat ccccgtcttc agtgttatag atagtatacg
     3061 gctcttcata gcgccacgcc gcgatcgaca gtgcatcccc ggcctgcatt ggggcgaaaa
     3121 tgaattgcat ag
//
LOCUS       DASZAK010000116         4062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_535230, whole genome shotgun sequence.
ACCESSION   DASZAK010000116 DASZAK010000000
VERSION     DASZAK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535230"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="VGT44_01980"
     CDS             <1..439
                     /locus_tag="VGT44_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579594.1"
                     /translation="GLALLLGIPTRLIPTPLFSRVVPTSPQDYAIWIVSALLLGPILS
                     LMTLYPMPSQKAAPGTRSLAGTGRAFGGALLSFFSVGCPVCNKIVVLLLGLGGAMTIF
                     NPLRPFLGLASIVLLGITLFLRVRVVRYGCRLPAHKTGANEEG"
     gene            complement(567..1367)
                     /gene="istB"
                     /locus_tag="VGT44_01985"
     CDS             complement(567..1367)
                     /gene="istB"
                     /locus_tag="VGT44_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEV2579595.1"
                     /translation="MKDLLQALRLGHVEVGLPDLYEQARLHGLTYEAFLRRVLLLEIE
                     GRKLTAQHKRLQAARLPVRKTLEEFDFAFQPGVNERLIWELAELSFVKTKTNIVFLGP
                     PGVGKTHLSIALSIKALEVGYSVLFTTLAHLASDLTTAPHAAALKGRMRRYLGPQVLV
                     IDEVGYTRLTEEQANLLFALVRDRYEHGSIILTSNTSFSEWGALLGNEVLATALLDRL
                     LHHAEAISISGKSYRMKNRLATGKTKGTASREERKEGISANGADPGAD"
     gene            complement(1364..2602)
                     /gene="istA"
                     /locus_tag="VGT44_01990"
     CDS             complement(1364..2602)
                     /gene="istA"
                     /locus_tag="VGT44_01990"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEV2579596.1"
                     /translation="MIRSGTIHTIHEMQTQGKSIREIARILGIARNTVRRYLRGKPEA
                     VPHPKRGSVLDAHKAQIRRWIQEDHLYNCETMLPRLQAQGYTGSLSTLKAFVHGLRPP
                     KAGHYPVLRYETKPGEQMQYDWGEFHYEKDGKDHKLYGFAAVLSYSRMRFITFVKRCD
                     TPTMIRCMLEAFEYFGGLPKAALTDRMKSVFLAMEDKTPKWNPVFSDFMATLGVAPRV
                     CKARTPQTKGKIERSVGIIKYGFWPGVHFTDLDDLNAQATAWCDRLNQRVHRTTRQVP
                     LDRWVEENLAPLPKDEVWERFGTEERRVSWDGFIAYDGVLYGLPSEPPVAGSLVRVRE
                     RHHQLLVFCGGQRMATLTKRPQSQDIVYHPDQFRTVAPASAIKAAAKPLGHQVSPPEV
                     ERRPLAEYDRLFGLEGQVVG"
     gene            complement(2775..3431)
                     /locus_tag="VGT44_01995"
     CDS             complement(2775..3431)
                     /locus_tag="VGT44_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2579597.1"
                     /translation="MPEENARLIALYQKQAKDYDQSGIHGLDAWRREAVKWLDLKQGD
                     LVVDIGCGTGLNFALLQEAIGETGRIIGVDLTDAMLEQARLRVTAQGWKNVELVQADA
                     ASYEFPTQVDGVLSTFALTFVPHRARVIQNGCRALAPGRRWVVLDMAWPAAWPVWLSP
                     FLFFLRLSRYGISREVIRRRPWQTVWSTMQQSLVEVERHPFWMGFFYLAWGTQPQPLA
                     "
     gene            complement(3554..>4062)
                     /locus_tag="VGT44_02000"
     CDS             complement(3554..>4062)
                     /locus_tag="VGT44_02000"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2579598.1"
                     /translation="GQNFPFYDPARVVRVEAVEPDEAMLAAARHRLEDAPVPITLTRA
                     PVEALPFPDAQFDTVVVTLVFCSVQDPERGLREIWRVLKPGGTLLMLEHVRAHAKMVA
                     WLQEVLVPVTTRWMGNDHWNRDTLQTVRHTGFQATEVRQLSGGLQPMLYVQAKRPQTQ
                     GEATVGRE"
BASE COUNT          737 a         1284 c         1199 g          842 t
ORIGIN      
        1 aggcttggca ctgctgcttg gcattcccac gaggctgatc cccacacccc tgttcagtcg
       61 cgtggtgcca accagcccgc aggactacgc gatctggatc gtttcagcgc tgctgctcgg
      121 gccaatactc tcgctcatga cgctttaccc catgccatct caaaaagcag cgccgggaac
      181 acgctctctg gcggggaccg ggcgagcgtt cgggggagcc ctgctctcgt tcttttccgt
      241 gggctgtccc gtctgcaata aaatcgtcgt cctgctgctg ggcctgggag gggctatgac
      301 catcttcaac cccctgcgcc ccttcttggg gttggcctcg atcgtcctgc tcggcatcac
      361 cctcttcttg cgcgtgcgtg tggtgcggta cgggtgtcgt ctccccgcgc acaagacggg
      421 tgccaacgag gagggatgaa gaaggcgatg atcattggcc gcgctcattg tcaacgccaa
      481 ccgccatttg acccatttcg ccaacgaaaa gtgacccacc cattccgctg ttttcctccc
      541 agtccttcct gtcgcaatgg agaaggttag tcggcgcctg ggtcagcgcc attcgcactg
      601 attccctcct ttctctcctc acggcttgcg gtgcctttgg tcttgcctgt cgccagccgg
      661 ttcttcatgc ggtaactctt gccgctaatc gagatcgcct cggcatggtg caggagccgg
      721 tcaagcagcg ctgtcgccag cacctcatta cccagcaacg ccccccactc ggagaagctg
      781 gtattgctcg ttagaatgat cgagccatgt tcgtagcggt cgcgcaccag ggcaaagagc
      841 aaattggcct gctcctcggt gagcctggtg tagccgactt catcaatgac gagcacctgt
      901 ggccccagat agcgccgcat gcgccccttg agcgcggcag catggggagc ggtcgtcaag
      961 tcgctcgcca ggtgcgccag ggtcgtgaac agcacactgt agcccacttc cagcgccttg
     1021 atcgaaagcg caatgctcaa atgcgttttc ccgacccctg gcggtccgag aaatacgatg
     1081 ttggtcttcg ttttgacgaa ggagagctcc gccagctccc agatcagccg ttcattgacc
     1141 cctggctgaa aggcgaaatc gaactcttcg agcgttttgc gcaccggcag gcgtgccgct
     1201 tgcaggcgtt tgtgctgcgc agtgagcttg cggccttcga tctccaacag caagacgcga
     1261 cgcaaaaagg cctcgtaggt cagcccgtgc agacgggcct gctcgtagag atcgggcagc
     1321 cccacttcca catgccccag gcgcagggct tgcaacaggt ccttcatccc accacctgcc
     1381 cttccagacc aaagaggcgg tcatactcgg cgaggggacg gcgttccacc tcgggcggcg
     1441 acacctggtg tccaagcggt ttggccgccg ctttgatggc cgaggcggga gccaccgtgc
     1501 gaaactgatc cgggtggtag acgatgtcct gcgactgcgg gcgcttggtg agcgtcgcca
     1561 tgcgctgccc gccacagaac acgaggagct ggtggtgccg ctcgcgcacg cgcaccagcg
     1621 agccggccac cggtggctca gacggcagcc cgtagaggac gccatcgtag gcaatgaagc
     1681 catcccagct gacgcggcgt tcctccgtgc cgaagcgctc ccagacctcg tccttgggta
     1741 agggcgccag attctcttcc acccagcggt ccagtggcac ctggcgtgtg gtgcgatgca
     1801 cgcgctggtt gagtcgatcg caccaggcgg tggcctgcgc attcagatcg tcaagatcgg
     1861 taaagtgaac gcccggccag aagccgtatt tgatgatgcc cacgctgcgc tcgatcttgc
     1921 ccttcgtttg tggtgtccga gccttacaga cgcgtggagc caccccgagc gtggccatga
     1981 agtcgctgaa gaccggattc cacttggggg tcttgtcttc cattgccaga aagacgctct
     2041 tcatgcgatc ggtcagcgcg gccttgggca agccgccaaa gtactcgaag gcttcgagca
     2101 tgcagcggat catcgtcggc gtgtcgcagc gcttgacaaa ggtgatgaaa cgcatgcgcg
     2161 agtaggagag cacggccgca aagccgtaga gcttgtgatc cttgccgtcc ttctcgtaat
     2221 gaaattcgcc ccaatcgtac tgcatttgtt cccctggctt ggtttcgtag cgcagcaccg
     2281 ggtaatggcc cgctttaggc ggacgcaacc catgcacaaa cgccttgagc gtcgagaggc
     2341 tgccagtata gccttgcgct tgcaggcgcg gcagcatcgt ttcgcagttg tacaagtgat
     2401 cttcctggat ccaccggcga atctgcgctt tgtgggcgtc gagcaccgag ccgcgctttg
     2461 ggtgaggcac cgcttccggc ttgccgcgta agtagcgccg cacggtattg cgagcaatgc
     2521 ccaggatgcg cgcgatttcg cgaatggact tcccttgtgt ttgcatttcg tgtatggtgt
     2581 gaatggtccc actccttatc actggcccat tcctccttgt gtctaagatg aacacaagaa
     2641 tagagcctgc tacggggtgg gtcaatcctt cctggcgttc ctgggtcaat tatagcttgg
     2701 cgctaacatc atccagcatt cctggaatgg gtatccagtg aaaatcctgc gtgacgagcc
     2761 tgggacgcca tctctcacgc gagaggctgg ggttgtgtcc cccaggccag gtagaagaac
     2821 cccatccaga atggatgccg ctcgacctcg accagggact gctgcatcgt gctccagaca
     2881 gtctgccagg gacgtcgtct gatcacctcc ctgctgatgc catagcgaga gaggcgcaaa
     2941 aagaacagga agggggagag ccatacgggc cacgcagccg gccaggccat gtctaacacg
     3001 acccagcgtc gtcctggtgc taacgcccga cagccgttct ggatcacacg cgctctatgc
     3061 gggacaaagg tgagagcaaa cgtcgagaga acgccatcca cctgagtggg gaactcatag
     3121 gaagcggcgt cggcttgcac gagttccacg tttttccagc cctgtgcggt gacgcgcagc
     3181 ctggcctgtt caagcatggc atcggtcaga tccaccccga tgatcctgcc cgtctcgcca
     3241 atcgcttcct gcaacagcgc gaaattcagt ccggtcccac agccaatatc caccaccagg
     3301 tcgccttgct tcaagtcgag ccacttgact gcttctcgcc tccaggcgtc gagcccgtgg
     3361 atgccgctct ggtcatagtc tttggcctgt ttctggtaga gcgcaatgag tctggcgttc
     3421 tcttcaggca cgctcttcct gctcctctct gcttcatggg cacaacacaa gcaggtgtgt
     3481 ttcatctggt atgccgggca tctcgcggac cttgctcccc tctccaggtc gctcagtcct
     3541 ctcacgaacg gtttcattcc cggcctactg tggcctcccc ctgcgtttgg gggcgcttcg
     3601 cttgcacata cagcatgggc tggagtcccc cgctgagctg acggacctcc gtggcctgga
     3661 aaccggtgtg tcgcactgtt tgcaaggtat cgcggttcca atggtcattg cccatccagc
     3721 gggtcgtcac gggaaccaac acctcctgga gccaggcgac catctttgcg tgtgcgcgca
     3781 catgttcgag catcaggagc gtccctcccg gcttgagcac gcgccagatc tcgcgcaggc
     3841 cccgttctgg atcctgtacc gaacagaaga cgagggtgac cactaccgta tcgaactgtg
     3901 catcgggaaa cgggagggcc tcgaccgggg cgcgggtaag cgtgatggga acgggagcat
     3961 cctccaggcg gtgcctggcc gcggccagca tggcctcgtc cggctcgact gcctccacgc
     4021 gcacgacgcg ggctggatcg tagaaaggga agttctggcc tc
//
LOCUS       DASZAK010000117         7591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_38121, whole genome shotgun sequence.
ACCESSION   DASZAK010000117 DASZAK010000000
VERSION     DASZAK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38121"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VGT44_02005"
     CDS             <1..442
                     /locus_tag="VGT44_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEV2579599.1"
                     /translation="ALMRFQSLPTFLFQPYLWIFRWRGRTHPQAWKKNAGTVGITAVG
                     MFGNGAGWGIPIAPPTALMVTVGGIAEKPGVVNGTIAIRDYLSLTVSFDHDIIDGAPA
                     ARFTQRLKELIESGYGLPDSTVELELTGAQGDVRQYGSATSHTY"
     gene            456..1943
                     /locus_tag="VGT44_02010"
     CDS             456..1943
                     /locus_tag="VGT44_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEV2579600.1"
                     /translation="MNIVVKTQYGEVRGCIDDGVDTFKGIPYAAPPFGANRLRPPQPV
                     APWSGVRDALAFGPKTPQPPYPPEVSALLPELATPGEDCLTLNVWSPDLGSARQPVMV
                     WIPGSAFEYGTAAMPWYDGSRFARDGIVCVTINYRVGVDGYLYLGKGNANRALLDQVA
                     ALQWVQENIAAFGGDPGNITIFGESAGAMSIGTLLSMPRAQGLFHRAIAQSGAAHPVI
                     SAVTAQRVGRYLAEKLGVEATREAIAAVPLDRMLAAQAELDADLFTHPDPERWGAEVV
                     AAMVLWQPVIDGNVIPARPIDRIAAGVGSDIDVMVGTNTDEWRLFLVAGGAIDHVTDE
                     ALVGTVAVYGLPVEATLAAYRAARPNTSAGDLLAAIQTDWWCRIPAIRLADAHAKSPS
                     ATYMYEFAWRSPQFNGLLGACHALEIPFVFDTLGNEIGLLWGNDPPQQLADTMHAAWV
                     AFATRGDPGWPKYDLKRRATMRFDTTSEVVDDPRSTERALWEGQR"
     gene            complement(2054..3277)
                     /locus_tag="VGT44_02015"
     CDS             complement(2054..3277)
                     /locus_tag="VGT44_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEV2579601.1"
                     /translation="MSSEIERAPSQLHHAAGAAPAVQAEAEPQGKRLLFVDNLRILLI
                     CGVLVQHLSDTYGSAGSWMYRDPATADTFTSTFLTALNSIGMAAGMGFFFLLAGYFTP
                     DSYDRKGGVAFLRDRIIRLGLPWLVYSLLFQPLVYYIAKGLPGSFWNFYPTYLHRVDS
                     IADGPIWFVELLLFFSILYVAWRWLARTRAQADSGKGTTPGYRAIFGFILALGIGSFV
                     VRIWWPQYDQPGPLNLPLGYLLQYVCFYVLGIVAYRRNWLFTLTPRMGRDWSLIALLA
                     TFPFYGVAIPYVLGGGTAGGQPGIELAGGFHWLSLAYALWESFVIVGVCIGLLALFRT
                     RLNHQGSLARGLTASVYTVYLIHPVVLVGFSYAFHVVTLYPLLKWGIAVLVTLPLCFL
                     ISGLIRKIPLANRVL"
     gene            complement(3315..3767)
                     /locus_tag="VGT44_02020"
     CDS             complement(3315..3767)
                     /locus_tag="VGT44_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579602.1"
                     /translation="MISQKYEAEMQSREPAMAPPGAVGRLLPPSWSKSALIGVKSLHS
                     LIYFSIEFCMGYLLYAGLKGREDRRSAIAAGVVAGESIIFVGNRFRCPLTGFAKKLGA
                     ASGSVIDIYLPGWLASNLVAIHIPLLALALGLHARNFLRRRSLHARVE"
     gene            complement(3797..4642)
                     /locus_tag="VGT44_02025"
     CDS             complement(3797..4642)
                     /locus_tag="VGT44_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579603.1"
                     /translation="MFRQWMRKRTLKHLFLWFALISLVVFSLAACGSSGSPGPSPTAS
                     TGATPSATPTPFRVTGIDLAVNPTSIAGTVCGSSASFTYTATFHIPAGTAGGTIQFMY
                     TVNNGRSSTSASVPVAPGETAKTYTFTSSGTLSPDHTYPGIAEVVVNSPNSVTSPQVK
                     PDGACVSPGAFQVTSIDLTVMPSSIAGLACNTTVTLKYTVTFHVAAAGPGGTIQFMYT
                     TNNGRSSTNGSVSVGAGQATVVYTFTTTGVLTPDHTFPGIAEVITSSPNQVNSPQVIP
                     TGQCS"
     gene            5019..6629
                     /locus_tag="VGT44_02030"
     CDS             5019..6629
                     /locus_tag="VGT44_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2579604.1"
                     /translation="MKDDVSNKQALPPAPSGERDAHAAPLLSETPSSMPVASPPDSRA
                     VRRGRVPRWLLATLVGILALAGGTGLLISLVQRPANPAPPSSTFQTTSCPFKLAAGVV
                     VGKDVVCGYLTVPEDHSRPQGPTIRLAVAIIKASYSIPDSDPVVDLQGGPGTELLATL
                     GPVLTPATLSNLQFGDRDLILFDQRGVGYSQPSLGCQASETLQACYDRLVRQGINLND
                     YNTIQNAGDVHDLARALGYHQVALWGISYGTRLALTVMRLFPADIRSVVLDSTSPPQV
                     DIDTGFPAATQRAFDTLFQGCAANPYCNQHYPRLQAVFARLVIDLNQHPATMQVTNPQ
                     TGQPVPVVITGDDVVNGLRNALYDTALIPKLPRVIYQLAHHDYSQVAAISEAANAALG
                     SDSVGMFYSVECSESTLTPQAMSAAVQMVEPETRHYYLTALQADYANCQLWRVQPVTA
                     AQWQPVTSAIPTLILAGEYDPATPPAYGMLAASTLSRSYFFLFPGTGHGVVGRNSCAT
                     SMFHTFLEVPTEKPDSTCMEGVGETLFE"
     gene            6716..7345
                     /locus_tag="VGT44_02035"
     CDS             6716..7345
                     /locus_tag="VGT44_02035"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEV2579605.1"
                     /translation="MKRHFSLSFVTSLLAIVCYLAFALLAFARYPLPYSPLSNWLSDL
                     GNPNLNPQGALFYDIGIVATAVVLLPFFLGLSRWKMRNNRGQRLMLLLTQGFGILGAF
                     AMAMSGLLPMNFLALHSFFSACLYILLGTAFAFSVAALRYYPAWPRWLLIVGALTALV
                     DLLYGVFHTIYVFEWITVALLLVYLGLLGAGTELCAPSVRHLAANETGL"
BASE COUNT         1547 a         2306 c         2208 g         1530 t
ORIGIN      
        1 agccttgatg aggtttcagt ccctgccgac ttttctcttt caaccctacc tctggatctt
       61 cagatggaga ggtagaacgc atccacaggc gtggaagaag aatgcaggga cggtaggtat
      121 cacggcagta ggcatgtttg ggaacggtgc gggctggggc atcccgatag cccctccgac
      181 agcgctcatg gtgacggtgg gtggcattgc ggagaaacca ggtgtggtga acgggaccat
      241 cgctatacgg gactatctga gtctgacggt cagttttgat cacgatatca tcgatggcgc
      301 tccggcagca cgtttcaccc aacggctcaa agaactgatt gagagcggct atgggctgcc
      361 ggactccact gtcgagttgg agctgaccgg agcgcaaggc gatgtacgcc agtatggctc
      421 cgcgacgagc cacacgtatt gaaagaggta gacccatgaa tatagttgtc aaaacacaat
      481 atggcgaggt gcgtggctgt atcgatgatg gcgtggacac ctttaaaggc attccttatg
      541 cagcgccacc ctttggcgcc aatcggcttc gccccccaca gccggttgcg ccctggagcg
      601 gcgtgcgaga cgcgctcgcc tttggcccga aaacacccca gcctccatat cccccagaag
      661 tcagcgcgct cctcccagaa cttgccaccc ccggcgagga ctgcttgacc ctcaacgtct
      721 ggtcccccga cctcgggtcg gccaggcaac cggtgatggt ctggattcct ggcagtgctt
      781 ttgagtacgg aacggcggcg atgccctggt atgacggcag tcgtttcgcc cgcgacggca
      841 ttgtctgcgt gaccatcaat taccgggtcg gtgtcgacgg ctacctctac ctgggcaagg
      901 gaaacgccaa ccgcgccttg ctcgaccagg tcgctgccct ccagtgggtg caagagaata
      961 tcgctgcctt tggtggcgat ccaggcaaca tcaccatctt tggcgagtcg gctggcgcga
     1021 tgagcatcgg cacgttgctg tccatgcccc gtgcccaggg gctttttcac cgggccatcg
     1081 cccagagtgg ggcggctcat ccggtgatct cagccgtgac cgcgcagcga gttggccggt
     1141 accttgccga gaagcttggc gtcgaagcaa cccgcgaggc gatcgcggcg gtcccccttg
     1201 atcgtatgct ggcagcgcaa gcggagttgg atgccgatct cttcacgcat cccgaccccg
     1261 agcgctgggg ggctgaggtg gtggcagcca tggtgctgtg gcagccggtg atcgacggga
     1321 atgtcatccc cgcgcgtccg attgatcgca ttgctgctgg cgtcggctcg gatatcgacg
     1381 ttatggtcgg cacgaacacc gacgagtgga ggctattcct ggtagcaggt ggcgcgatcg
     1441 accacgtcac tgacgaggcc ctggtcggaa ctgtggcggt ctacgggctc ccggtcgaag
     1501 cgactttggc cgcctatcgc gcggcacgtc ccaacaccag cgccggcgac ctgctcgcgg
     1561 ctatccagac cgattggtgg tgccgtatcc ccgccatccg cctggctgat gcacatgcga
     1621 agagcccctc agccacctac atgtacgagt tcgcctggcg ctcgccgcag ttcaacggac
     1681 tcctcggcgc ttgccatgcg cttgagattc ccttcgtctt cgacacgctg ggcaacgaga
     1741 ttgggctgct ctggggaaac gaccctccac aacagctcgc tgacaccatg cacgccgcct
     1801 gggttgcctt tgccaccagg ggagaccctg gttggccgaa gtacgatctg aagcgcaggg
     1861 ccaccatgcg cttcgacaca acgtcagagg tcgtagacga tcctcgctcc acggagcgag
     1921 cgctgtggga aggccagcgg tagcttccgc taccgcttgt aacttttcca tctcacaact
     1981 gttttgcaag ggatgctcac acgaggtgtc aagcatccct tcggtcggtt tcggggatgc
     2041 ttaatcttgt ccttcacaac actcggttgg ctaagggaat cttgcggatc aagccgctga
     2101 tcaggaagca gagtgggagc gtgacgagta ccgcgatgcc ccatttgagc aggggataga
     2161 gcgtcaccac gtggaaggca tacgaaaacc cgacgagcac cacaggatgg atcaggtaga
     2221 ccgtgtaaac actggcggtc agacctctcg ccaggctccc ctgatgattc aagcgcgtgc
     2281 ggaagagcgc cagcaacccg atgcagacgc ccacaatcac gaacgactcc cacagcgcgt
     2341 aggcaaggga cagccagtgg aagccacccg ccaactcgat gccaggctgt ccccctgctg
     2401 ttccccctcc aagcacatag gggatcgcca cgccataaaa agggaaggtg gcgaggagtg
     2461 cgatcaacga ccagtcccga cccattctgg gagtgagggt gaacaaccag ttgcggcggt
     2521 aggcgacgat ccccagcaca tagaagcaga catactggag gaggtagccc agtggcaggt
     2581 tgagcggacc aggctgatcg tattgcggcc accagatgcg caccacaaag ctcccgatac
     2641 ccagcgccag gatgaaaccg aagattgccc ggtagccggg cgtcgttcct ttcccgctgt
     2701 ccgcctgcgc tcgcgttctc gcgagccaac gccaggcaac atacaggatg ctgaagaaga
     2761 ggagcagttc gacgaaccag atcggcccat cggcaatcga gtccacccgg tgcaggtacg
     2821 tggggtaaaa gttccagaag gagccgggca ggcccttcgc gatgtagtag accagcggtt
     2881 gaaacagcag cgagtagacg agccacggga ggcccaggcg gatgatacgg tcgcgcagaa
     2941 aggcgacgcc gcccttgcga tcataggagt ccggtgtaaa gtagccggcg agcaagaaaa
     3001 agaaccccat gccagcagcc ataccgatgc tgttcagggc cgtcaggaag gtcgaagtga
     3061 acgtatcagc ggtggcaggg tcgcgataca tccaggaacc ggccgagccg taggtatcag
     3121 agaggtgctg caccagcacc ccacaaatta gcaagatgcg caggttatcc acaaacagca
     3181 ggcgcttgcc ctggggctct gcctctgcct gtaccgccgg ggcggcgcct gccgcatgat
     3241 gaagctgaga aggtgcccgc tcaatttcgg atgacatcgt cttctccttt cccatagggg
     3301 tgctgctcct tcgctcattc taccctggca tgaagggatc tcctgcggag gaagtttctt
     3361 gcgtgcagac ccagcgccaa agcgagtagc ggtatgtgga tcgctacaag gtttgaggca
     3421 agccagcccg gcaggtagat gtcgatcacc gaaccgctcg cagctccaag tttctttgcg
     3481 aaacctgtca gaggacagcg aaagcggtta cccacaaaga tgatgctctc acccgcgacg
     3541 accccggctg cgattgcgct tcgtctatcc tcccgcccct tcagccctgc gtagaggagg
     3601 taccccatac agaactcaat agaaaagtag ataagcgaat gcagtgactt cacgccaatg
     3661 agcgccgatt tactccacga tggtggaagc agtctaccaa ctgcaccggg tggggccatc
     3721 gccggttctc tcgactgcat ctcagcttca tacttttgtg agatcatgag agaccctcct
     3781 ttgccagcca ggtcgatcaa ctgcactgac cggtcggtat cacctggggc gagttgacct
     3841 ggttgggact gctcgttatc acctccgcga tgcctgggaa ggtgtggtcg ggggtgagaa
     3901 cacctgtggt cgtgaatgta tagaccactg tcgcctgccc tgcccctacc gaaacgctgc
     3961 cattggtgct ggagcgccca ttattggtcg tgtacatgaa ttgaatcgtg ccgccgggac
     4021 cagcagctgc gacgtggaag gtcacagtgt atttcagggt cacagtggtg ttgcaggcca
     4081 gacccgcgat tgaagaaggc ataaccgtca ggtcgatgct cgttacctga aacgcaccag
     4141 gcgaaacgca ggcaccgtct ggcttgacct gtggcgaagt gacactattc ggactattca
     4201 ctaccacctc tgctatccct gggtaggtat gatccggaga gagcgtcccg gaactggtaa
     4261 acgtataggt tttcgcggtc tctccaggag caacaggaac actcgcgctc gtcgaggaac
     4321 gtccattgtt cacagtgtac ataaactgaa ttgtcccgcc agccgtgcct gcgggaatgt
     4381 ggaaggtagc agtataggta aagctcgccg aactcccgca taccgtccct gcaatgctcg
     4441 tcggattcac agctaagtca atgcctgtca ccctgaatgg tgttggtgtg gctgatggcg
     4501 tagccccggt gcttgccgta ggtgaagggc cagggctgcc gctacttcca cacgccgcca
     4561 gtgagaagac gaccagggag atgagggcaa accagagaaa aagatgcttc agcgttctct
     4621 tgcgcatcca ttgcctgaac attgtgtgcc tccttcgatg ggatctgaat agcaggtgag
     4681 cacgatactc ccattcaatg aactcttttg ctcatctgct gatctttagc ctaccatgcc
     4741 ttcatcacag tgttcgcaca gtttggaagg aggagatcac aggaccatca cacttcgtgg
     4801 agtccctcgc tgctccagca aactgagagt aagaaccgtc tttcgatagc catcgatgaa
     4861 ttcgtgacga ttctgtgcgt tcagatgaac agtatgtgac cgcctcgtga gagcggactg
     4921 ttactttgta aggtggaaga ctatgagcag cggagtaaaa acaagaaaaa taacagccgc
     4981 tgtcgccagt tcatgacatg ccaacgagga gaaccgctgt gaaagatgat gtgtccaaca
     5041 aacaagcgct tccaccagcc ccgagcggag agcgggatgc tcacgctgcc ccattgcttt
     5101 ctgaaacccc ttcaagtatg cctgtcgcat ctccaccgga ttcacgagcg gtaagacgag
     5161 gacgagtgcc gcgctggctg ctcgcgaccc tggtgggcat actggcgctg gccggtggga
     5221 ccggcctgtt gatctctctc gtacagcgac cggcgaaccc tgcgcctccc tcctccactt
     5281 ttcagacgac ctcctgcccg ttcaaactgg cggcaggtgt ggtggtgggg aaggatgtcg
     5341 tctgtgggta cctgaccgta cccgaagacc acagccgccc gcagggtccc accattcggc
     5401 tggcggtcgc catcatcaag gcttcgtact ctattcccga ttccgacccg gtcgtcgatc
     5461 tgcaaggcgg acctgggacc gagctgctgg cgactctcgg ccctgtcctc acaccggcca
     5521 ccctctccaa cttacagttc ggggaccgcg acctcatcct gtttgaccag cgcggggtgg
     5581 gctactcgca gccctcgctc ggctgtcagg ccagcgagac cctgcaggcg tgctacgacc
     5641 gcctggtgcg gcagggcatc aacctcaacg actacaacac catccagaac gccggggatg
     5701 tgcatgacct ggcgcgcgcc ctgggctacc accaggtcgc cctgtggggc atctcctacg
     5761 gcacccggct ggccctcacc gtgatgcgcc tcttcccggc ggatattcgt agcgtcgtcc
     5821 tcgactcgac ctcccctcca caggtcgaca tcgacaccgg tttcccggct gcgacccagc
     5881 gggcgtttga tacgctcttt cagggctgtg ccgccaaccc ctactgtaac cagcactacc
     5941 cgcgcctgca ggcggtcttc gcgcgcctgg tcatcgacct gaaccagcat ccggcgacca
     6001 tgcaggtgac caacccgcag accggccagc ccgttcccgt cgtcatcacg ggcgacgatg
     6061 tggtgaacgg actgcgcaac gccctctacg ataccgcgct cattcccaaa ctgcccaggg
     6121 tgatctacca gcttgcccac cacgactact cgcaagtggc ggcgatcagc gaggcggcca
     6181 atgccgcgct tggctcggac agcgtgggca tgttctactc ggtcgagtgc agcgagtcga
     6241 ccctcacccc gcaggccatg tcggccgctg tgcagatggt agaaccagag acgcggcatt
     6301 actatctcac ggcactgcag gcagattacg ccaattgcca gctctggcgc gtccagccgg
     6361 tgaccgcggc gcagtggcag cccgtcacca gcgccattcc cacgctgatc ctggccggcg
     6421 agtacgaccc ggcaacgccg ccggcctacg ggatgctggc ggccagcacg ctctccagga
     6481 gctacttctt cctgtttccc ggcacagggc atggcgttgt gggacgcaac tcctgcgcca
     6541 cgagcatgtt ccatactttt ctcgaagtcc ccactgagaa accggatagc acctgcatgg
     6601 agggggtggg agaaacgctc ttcgaataat atgcggtcag gtctatcgcc tggggaacct
     6661 gttggccagt tcagcttgtt tttgacagtg gaagaaagat tcggaggagt atcccatgaa
     6721 gagacatttt tctcttagtt tcgttaccag cctgctcgct attgtgtgct acctggcgtt
     6781 tgccctcctg gctttcgcgc gctacccttt gccctactcg ccgctgagca attggctcag
     6841 cgatctaggc aatcccaact tgaatccaca gggggcgctc ttctacgaca tcgggatcgt
     6901 cgcaaccgcg gtcgtgctgc tgccgttctt ccttgggtta tctcggtgga agatgcgaaa
     6961 caaccggggg caacgcctca tgctgctcct gacgcaggga tttggaatac tcggagcttt
     7021 tgcaatggcc atgagtggcc tcttaccaat gaattttctg gcgctccaca gtttcttctc
     7081 cgcgtgcctg tatattctcc tggggacggc gttcgccttt tccgttgccg cgctccggta
     7141 ttatcccgcc tggccgcgat ggctcctcat cgtaggcgcc ttaaccgctc ttgtggatct
     7201 gctgtacggc gtctttcata cgatctatgt gtttgaatgg attacagtcg ccctgctgct
     7261 agtctatctt ggcctgctcg gcgccgggac ggaactctgt gcgccgtctg tgaggcatct
     7321 cgctgcaaat gagacaggtt tgtgatatct gactgctacg ctcaaagtat gaaaaacaca
     7381 aaagcgcaag aaaagggcga aatgttgcac ataggcagcc aggataaacc aaccacgtca
     7441 gtttccactg atgataccac cgatcaggcg ataacgggcg tctccccact ggaatcgccg
     7501 ccttccaggg cgcgtcgtct ctggcgcacc gcgcgaagtt atcccattcc tttgggatcc
     7561 ttgctcctgc ttgccgcgag cctgatactc t
//
LOCUS       DASZAK010000118         2216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_357984, whole genome shotgun sequence.
ACCESSION   DASZAK010000118 DASZAK010000000
VERSION     DASZAK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357984"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1200)
                     /locus_tag="VGT44_02040"
     CDS             complement(<1..1200)
                     /locus_tag="VGT44_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579606.1"
                     /translation="MKQSALDAAAALLVEIAGPEPVHIEMSSRGPKKYYDVHRALNEQ
                     DARAHLAGRVTKGATLRHPAGMTRALCYDADTPDDWLRLVEAAWQLAEHGYRPLLEVS
                     PAQDDRHAGGGHLWIIYTALVNAAWAQRQVVQIAPALHALHENWPNTSGNKVRLPGGR
                     YVRPEVRAWCRLYDAQGTRLSSDGPGAARVLLDWQTPAELVPAFPDAEPNGETGELAP
                     LPNGAVGQRGRSAPLPSAPDAPGVDEQWQRKHGRYFWFQFTPAQLASWYNERTPIEDL
                     LPREKNGMGLASWRGERTASVGIRADGWVDFGASARRPDGKQDGGDALELQVRISQQA
                     KPEVMRRVGRQLVHEAREVLESAAWRQEQPPAWVQPFLTEAGWQHYHALRAEAARAAL
                     HRGVGGFS"
     gene            complement(1197..2033)
                     /locus_tag="VGT44_02045"
     CDS             complement(1197..2033)
                     /locus_tag="VGT44_02045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579607.1"
                     /translation="MERETILAQVKTALQVRGWTLHLQQRRNRPYAYAARRTGTRTED
                     VYLGPLDDLTAMLARIEALPNRKPVPIGQRSEKHDQTNASERDPERLRLQSDLLELGR
                     RMGYRRFIVVPFFIDPGLDAWTRYSMTTDTPTLGRVIQAARVYSGHLKEVGLMQEPNT
                     ESSPPCSTLYDGAIMDRHVPNTNAPGSYDPLGNYWCADCAEHCEVMYHGAQLGWPQVS
                     YFPRNPPGMQALMVKAGADAWREYLRIRGKQAARVVREKTQDLLKAQGLEALIGREES
                     RS"
     gene            complement(2037..>2216)
                     /locus_tag="VGT44_02050"
     CDS             complement(2037..>2216)
                     /locus_tag="VGT44_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579608.1"
                     /translation="PLDLQERVSTPKGPGMVVQRWQGLIGVKLDETHEVIYFRGKDEI
                     ARLVPTTRQHSAKET"
BASE COUNT          356 a          727 c          693 g          440 t
ORIGIN      
        1 agaaaaacca cctacccccc gatggagagc ggcgcgtgct gcttccgcgc gtagggcgtg
       61 gtagtgctgc cacccggcct ccgtcagaaa gggctgcacc caggccgggg gctgctcctg
      121 gcgccaggct gcactttcga ggacttcccg cgcttcgtgg accagctggc gcccgacccg
      181 gcgcatgact tccggtttgg cctgctgact gatgcgcacc tggagttcca gtgcgtcgcc
      241 gccatcctgc ttgccatcgg gacggcgggc gctggctccg aaatccaccc atccgtctgc
      301 tcgtatgccc acactggcgg tgcgctcgcc gcgccaggaa gcaaggccca tgccgttctt
      361 ctcccgcggt aggaggtcct ctatcggggt gcgttcgttg taccagctcg ccagctgcgc
      421 cggggtgaac tgaaaccaga agtaacgccc atgcttgcgc tgccactgct catcgactcc
      481 aggagcgtcg ggcgcgctgg gcaagggtgc cgacctgcca cgttgcccaa cagcaccgtt
      541 gggcaacgga gcaagttcac ccgtttcccc attgggttcg gcatcaggaa acgctggaac
      601 gagttcagcc ggggtttgcc agtccaggag cacgcgcgcc gcgcctggtc catcgcttga
      661 caagcgtgta ccctgagcat catagagccg gcaccaggcg cgcacctcag gccgaacata
      721 ccgcccgcca ggcagccgca ctttgtttcc gctcgtgttg ggccagtttt cgtgaagagc
      781 gtggagcgcg ggcgcgatct gcacgacctg gcgctgagcc caggccgcgt tgaccagcgc
      841 cgtgtagatg atccacagat gcccgccacc ggcgtgccgg tcgtcttgtg ctggcgagac
      901 ctccagcaga ggccggtagc cgtgttcggc caactgccag gccgcctcta ccaggcggag
      961 ccagtcgtca ggcgtgtcgg cgtcgtaaca gagcgcacgg gtcatcccgg cgggatgacg
     1021 taaggttgcg ccttttgtga cgcggccagc caggtgtgcg cgggcatcct gttcgttgag
     1081 cgcgcgatgc acgtcgtagt atttcttggg gccccgcgac gacatctcga tatgtaccgg
     1141 ctccgggccg gcgatctcca cgagcagcgc ggccgctgcg tcaagcgctg actgtttcat
     1201 gacctgctct cctcccggcc aatcagggct tccaaccctt gcgccttgag caggtcctgc
     1261 gtcttctctc gcacgacgcg cgcggcctgt ttcccgcgta tccgcaggta ttcgcgccac
     1321 gcatcggctc cggctttcac catcagcgcc tgcattcctg ggggattacg gggaaagtag
     1381 gagacttggg gccagcccag ctgcgcgccg tggtacatca cttcgcagtg ctccgcgcaa
     1441 tccgcacacc agtagtttcc cagcgggtca taggagccgg gggcatttgt gttcgggacg
     1501 tgccggtcca tgatcgcccc atcgtaaaga gtcgagcagg gagggctgct ttccgtattt
     1561 ggctcttgca tcaatcccac ctccttcagg tgtccagagt acacgcgagc cgcctgaatc
     1621 accctcccaa gtgtcggagt gtcggttgtc atgctgtagc gcgtccacgc atctaagcca
     1681 gggtcgatga agaacgggac cacgatgaac cgccgatagc ccatgcgccg cccgagttcc
     1741 agcaggtcgc tttgcaagcg caaccgctcc gggtcacgct cgctcgcgtt tgtctggtca
     1801 tgtttttctg agcgttgccc aatgggaaca ggtttgcggt tgggcaacgc ttcgatgcgg
     1861 gccagcatgg cggtcaggtc atcgagtggg cccagataga catcttcggt gcgtgtgcca
     1921 gtgcgcctgg cagcataggc atagggcctg ttgcggcgct gctggagatg aagcgtccaa
     1981 ccacgaactt gcagtgcagt cttcacctgc gcgaggatgg tttctctctc cacgggtcag
     2041 gtctcctttg ctgagtgttg ccgtgtcgtt ggcaccaggc gcgcgatctc atccttgccc
     2101 cggaagtaga tcacctcgtg cgtctcgtcc agcttgactc caatgaggcc ctgccagcgc
     2161 tgcaccacca tgccagggcc tttcggggtg ctcacccgct cctgcaaatc aagggg
//
LOCUS       DASZAK010000119         1663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_642031, whole genome shotgun sequence.
ACCESSION   DASZAK010000119 DASZAK010000000
VERSION     DASZAK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642031"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VGT44_02055"
     CDS             <1..372
                     /locus_tag="VGT44_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV2579609.1"
                     /translation="AIGQISPGPNGLWVISLGYLTYGFLGALLALIAITIPALLVLVI
                     SAGYTRIEHQTWVQGAMHGVSLAVVGLLLAVVWTILHQPGVDWKGLLIAGGAFGLALS
                     RKVNILIILGLAGLAGYFLYR"
     gene            complement(467..>1663)
                     /locus_tag="VGT44_02060"
     CDS             complement(467..>1663)
                     /locus_tag="VGT44_02060"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579610.1"
                     /translation="LEVWTTETTHRDGQQGGLPLSTEQSLNIYDILCHFTADSGAIRQ
                     AEFFVYRASDREALQGALERYQSGAPIEPTTWIRATAKDVVLIRSLGIQETGMLASAS
                     DYHTFHKFKPGGRNQAAQTYLEALKMTLDAGIRPRLHLEDATRAPMDFMRPFIENVQE
                     ISAAYGPELRPKFRICDTMGLGLPFDDVALPRSIPRIFEEIRKLGLQPADLEFHPHND
                     TSLVVANCLAAVRAGCAVINGTSLGKGERTGNAPLESVLLHLIGMGYFRENQPDFTAL
                     NELVALYAEMGEPVPPKYPLYGRDAHRTRAGVHADGLNKFWWMYAPFNVPQLLGRPLE
                     VSLTKESGLAGLIYLIRQHTGLELTKDDLRLQKAYAWLMGEFDNGRQTSVEWEELKPL
                     WENGFQ"
BASE COUNT          365 a          442 c          456 g          400 t
ORIGIN      
        1 gctattgggc aaatcagccc cggcccgaac ggattatggg taatcagcct gggctatctc
       61 acctacggtt ttcttggagc attgctcgcc ttgattgcca tcactatccc cgcgctcctg
      121 gtactcgtca tctctgcagg ctatacccgc atcgaacatc aaacctgggt acaaggcgct
      181 atgcatggtg tttcgctcgc cgtcgtcggc ctgctgctcg ctgtcgtgtg gactattctg
      241 caccaacccg gagtagactg gaaagggctg ctcatagccg ggggcgcctt cgggctggcg
      301 ttgagtcgca aagtcaacat tctgattatt ttaggtttgg cagggctggc gggatatttt
      361 ttgtatcgct aagggctcct tgtttcaatt gccgtttgag agaagagatg ctcgcgacaa
      421 cttctgttca caaccatcta caggcgcagg aaatgccgcg ctgcttttac tgaaacccat
      481 tttcccacag gggtttgagt tcttcccact cgacactcgt ctggcgacca ttgtcaaatt
      541 cccccataag ccaggcgtaa gctttctgca ggcgaagatc gtctttggtg agttcaagtc
      601 cggtgtgctg gcgaatgagg tagattaagc ccgcgagtcc gctctctttt gtgagcgata
      661 cctcaagtgg tctaccaagg agctgtggaa cgttgaaagg agcatacatc caccagaatt
      721 tgttgaggcc gtcagcatgc actcctgccc tggtgcggtg tgcgtcgcga ccatagagcg
      781 ggtacttagg gggaaccggc tcgcccattt cggcgtaaag agccacaagc tcgttcaggg
      841 ccgtgaaatc tggttgattc tcgcggaagt aacccatccc aatgagatgt aacaagacgg
      901 attcgagtgg ggcattaccg gtacgctcac ctttaccaag agaagtgcca ttgatgacgg
      961 cgcatccagc acgcactgcc gccaggcagt ttgcgacaac aagcgaggta tcgttatgcg
     1021 gatggaactc caggtcggca ggttggagac ctagtttgcg tatctcctca aagatacgtg
     1081 gaatgctgcg aggtagcgcc acatcgtcaa acggcagtcc aagtcccatc gtatcgcaaa
     1141 tgcgaaattt cggacggagt tctggaccat aagcagcgct tatttcctga acgttctcga
     1201 taaatgggcg cataaaatcc atcggagcgc gggtggcgtc ttcaagatgc agcctggggc
     1261 gaattcctgc atccagcgtc atcttcaatg cctccaggta ggtctgggca gcctgatttc
     1321 tgcctccagg cttgaacttg tggaacgtat ggtaatccga agccgaggca agcattcccg
     1381 tctcttggat acccaggctg cgaataagca caacatcctt cgctgttgcc cggatccagg
     1441 tggtcggctc aataggagca ccactctgat agcgctctaa agcaccctgg agcgcctcgc
     1501 gatccgaggc acgatagaca aaaaactctg cttgtctgat agctccagaa tcagctgtga
     1561 agtgacaaag gatatcgtaa atgttgaggc tctgctcggt cgatagtggg agtcctcctt
     1621 gctgaccgtc acgatgagtt gtctcggtgg tccatacctc gag
//
LOCUS       DASZAK010000120         3772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_499910, whole genome shotgun sequence.
ACCESSION   DASZAK010000120 DASZAK010000000
VERSION     DASZAK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499910"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VGT44_02065"
     CDS             complement(<1..443)
                     /locus_tag="VGT44_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579611.1"
                     /translation="MRGSVTLAFLSSIPTPVLIAVAALVIAVVVGGVAFFLFRGGKKK
                     PGAASATGAPDWQRQQAAAGQQNPWGQQQQQPWGGQQPGSQAGWGDANAAQQPAGWGT
                     PGAPQQPSAAGWGTPAAQQPQQGGWDAAQQQQPAGWGTPAAQQPPS"
     gene            complement(553..1866)
                     /locus_tag="VGT44_02070"
     CDS             complement(553..1866)
                     /locus_tag="VGT44_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2579612.1"
                     /translation="MSGDMVGRVIKGRYKLIDELGRGSFATVYIARDTKTNRIYAMKI
                     MHYDLADDGEQLARFQREAHILLSLSDPHIVRIFEYGDENELHYIMMDYIDGQNLKYH
                     ILTNGPMEPGRALDYTHQIAEGLDAAYKHGVVHRDIKPQNIVINSKDVARITDFGLAR
                     SRETVTLTQSNVFMGTAYYIPPEQAESGRAADIRSDLYSLAAVLFEMLSGHPPFEGDT
                     AVDIVVKHMNEPVPSICRLRPDLPSELDGFLRKAMAKAPADRYGTPQEFLTALEQVQP
                     LVPGIERQKTMERAALGAFQQDGRASAEPLEPQQTPKSSQQEQQAGREQSGAVGGNNA
                     PRKLARLLVLSSNQTIPLNGEMMVVGRHDPVLGIYPEINLADKTVGRRHAYLRYQHGQ
                     YSVEDLNALNKTRLNGVILPPHEERSLKDGDMLRFGSVEVRFELR"
     gene            2123..2902
                     /locus_tag="VGT44_02075"
     CDS             2123..2902
                     /locus_tag="VGT44_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HEV2579613.1"
                     /translation="MPIRGVLLDIDGVLHVGMQPIPGAADTLRWLERHGYATCFVTNT
                     TTSSRASLVQKLQAIGLPIAEERVVTAPVATASFIRQHYPGKRVWLLTKGNTAEDFWG
                     IDLVQDHADIVVIGGAEELLTYEAMNRAFRMLMDGALLLATHSNLYWRTLDGLQLDSG
                     AYIRALEAATGQQATVLGKPARAFFEQALRSINLAASEAVMVGDDIENDIGGAQQVGL
                     RAALVCTGKHNASSPLLERVRPDAILPSIADVPGWLEKQST"
     gene            2954..>3772
                     /locus_tag="VGT44_02080"
     CDS             2954..>3772
                     /locus_tag="VGT44_02080"
                     /inference="COORDINATES: protein motif:HMM:NF023605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NotI family restriction endonuclease"
                     /protein_id="HEV2579614.1"
                     /translation="MPKHPLAEVFGFPTDNVSLDAARYRKNKLCPYNNRVPSCTKDKI
                     ENPLGVCSVFDNDAIAITCPVRFRQDWLIAEDAASFFFSTGKSWTSLTEIRINDKHGR
                     SAGNIDIVLVAYDDRGRIVDFGSLEVQAVYISGNVRKAFDYYMEDPVNHAAMDWTKQR
                     FYPRPDYLSSSRKRLAPQLIYKGGILHTWGRKQAVAVDSSFFHTLPAMEEIDQSEAEI
                     AWLVYDLQRDYQQNRYHLVRSKTVYTRFESALDKITRAEAGDVQMFIEHLQERLD"
BASE COUNT          796 a         1039 c         1052 g          885 t
ORIGIN      
        1 gaaggcggct gttgggcggc gggcgtaccc cagccggcgg gttgctgctg ttgggcagca
       61 tcccagccgc cctgttgcgg ctgctgagcg gcgggcgtac cccagccggc ggcggagggt
      121 tgttggggcg cgccaggcgt accccatcca gcgggctgct gagctgcatt cgcatcgccc
      181 cacccggcct ggcttccagg ttgttgccca ccccagggct gctgttgctg ctgtccccac
      241 ggattttgct ggcccgctgc tgcctgctgg cgttgccagt cgggcgcgcc tgtcgcggag
      301 gccgctccgg gtttcttttt gccgccgcgg aacaggaaga aggcgactcc cccgacaact
      361 accgcaatca ccaacgctgc cactgcgatc agcactggtg tgggtattga agaaagaaac
      421 gctaatgtga cggaccctct cataactttc acctctctca ttgtaggggc gtaccctgcc
      481 aaagtgatta cgcccgatag tgacatgggt acgaaagttg atttcgcata cctcgcaccc
      541 ctacaatagc atttaacgca actcgaatcg cacctcaaca ctgccaaagc ggagcatatc
      601 gccatccttg agcgatcgtt cttcgtgcgg cggtaagatg actccattta accgggtttt
      661 atttaaggca ttcaggtctt caacgctata ctgcccgtgc tggtagcgca ggtaggcgtg
      721 gcgccttcca accgtcttgt cagcaagatt tatctcggga tagatgccaa gcactggatc
      781 gtggcgcccg accaccatca tttcgccgtt gagcggaatg gtttgattac tggaaagaac
      841 tagcagacgc gccagttttc gcggggcatt atttcctcca accgcgccgc tttgctcacg
      901 cccggcctgc tgttcctgtt gggaggactt tggcgtctgt tgtggctcca gtggctcagc
      961 agatgctctc ccgtcctgtt gaaacgcgcc cagtgcggcc cgctccatag ttttctgccg
     1021 ctcgatgccg ggaaccagtg gttgcacctg ttccagtgcg gtgagaaact cctgcggagt
     1081 gccgtagcgg tcagctggcg ccttcgccat ggcttttcgc aggaagccat ccagttcgga
     1141 gggtaaatcg ggacgcaggc ggcaaatcga tgggaccggt tcattcatgt gtttgaccac
     1201 gatatcgaca gcggtgtctc cctcgaaggg tggatgaccg gagagcatct cgaacaggac
     1261 ggctgcgagc gaatacaggt cggaacgaat atccgcagcg cgaccacttt cagcctgctc
     1321 tggcggaatg tagtacgcgg tccccatgaa gacgttggat tgtgtcagtg tgaccgtctc
     1381 tcggctgcgc gccaggccaa aatcggtgat acgggcgaca tccttactgt tgatcacgat
     1441 attctgcggc ttgatgtcgc gatgcacaac gccgtgtttg taggcggcat caagtccctc
     1501 cgcgatctgg tgcgtgtaat ccagcgcccg ccccggctcc atcggcccat tcgtcaggat
     1561 atggtatttc aggttctggc cgtcgatgta gtccatcatg atgtagtgca gctcgttctc
     1621 atccccatac tcaaagatgc gcacaatgtg tgggtcgctg agtgacagga gaatgtgcgc
     1681 ttcgcgttga aagcgcgcca gttgttctcc gtcgtcggcg aggtcgtagt gcatgatctt
     1741 catggcgtag atgcggttgg ttttcgtgtc acgcgcaata tagacggtag caaaactgcc
     1801 ccgccctaac tcatcaatga gtttatagcg acccttaatg acacgtccaa ccatgtcacc
     1861 gctcatacac ttcccttcct gaaaggctac tcctcgcggc tattatacat cattcagtaa
     1921 cgaatgtcat ggctcgtatt cccatagcaa gttgacccat cgtgatgtac aaacggacta
     1981 ctcatagtac cagtcatgct aatgggtaaa gtctcctata gctgcattga cagaagcgcc
     2041 tggttttcct acaatgaaac cagtatgatt gtgtgtttcg ctggatgaga gcgtcgctga
     2101 aggagcaaag aggatcagtc aaatgcctat tcgcggcgtt cttctcgaca tcgacggcgt
     2161 gttgcacgtc ggtatgcagc cgatccctgg cgcggctgac acgctgcgct ggcttgagcg
     2221 tcatggctac gccacctgct tcgtcaccaa cacgacaacc agttctcgcg cctcgctcgt
     2281 gcaaaagctc caggcgatcg gtttacccat cgcagaagag cgagtcgtga ccgcgccggt
     2341 cgccaccgcc agcttcatcc gccagcatta ccccggcaag cgcgtctggc tgctgaccaa
     2401 aggcaacacc gccgaggact tctggggcat cgatcttgtc caggaccatg ccgacatcgt
     2461 ggtgatcggc ggagcggaag aactgctcac gtatgaagca atgaaccgtg ccttccgtat
     2521 gctgatggac ggcgcgctgc tgctggccac gcactccaac ctctactggc gcaccctgga
     2581 cggcctgcag ctcgacagcg gggcctacat tcgcgccctg gaagcggcga ccggccagca
     2641 ggcgaccgtc ctgggcaagc ccgcccgcgc cttcttcgag caggccctgc gctccatcaa
     2701 cctcgccgcg agcgaggctg tgatggtcgg cgacgacatc gaaaacgaca ttggcggcgc
     2761 gcagcaagtc ggcctgcgcg ccgcgctggt ctgcaccggt aagcacaacg ccagctcgcc
     2821 gctgcttgaa cgggtgcgac cagatgcgat tttgccgtcg atcgcggatg tgccggggtg
     2881 gctggagaag caaagcactt gacacatcag ggacaataga agcacaaaac tattttgatt
     2941 tgaggaaggc gctatgccga aacacccact ggcagaagtt tttggtttcc ccacagataa
     3001 cgtttcattg gatgctgcgc gctatcgcaa aaacaagctt tgtccataca ataatagggt
     3061 gccttcatgc acaaaagaca agattgagaa tcccctgggt gtttgtagtg tgtttgataa
     3121 tgatgccata gctattacct gtcctgttcg ttttcggcaa gattggctca ttgctgagga
     3181 tgctgcttct ttcttctttt cgacaggaaa atcttggaca tctctaacag agattcgtat
     3241 taatgataag catggtaggt cagcagggaa tatcgatata gtgcttgttg cctatgatga
     3301 tcgaggacgc attgtagatt ttggttcact tgaggttcaa gctgtgtata tttctggcaa
     3361 tgtgcgcaag gcttttgatt actacatgga agaccctgtt aatcatgcgg ctatggattg
     3421 gacgaaacag cgtttctacc cacgtccaga ttatctatct tcttctcgga aacgattggc
     3481 cccgcagcta atttataaag gtggtatcct tcatacgtgg ggtcggaaac aggctgtagc
     3541 tgtagacagc agttttttcc atactttgcc tgcaatggaa gaaatagatc agagtgaagc
     3601 ggaaatagcg tggttagttt atgatttgca acgggactat caacagaatc gatatcacct
     3661 ggttcgttct aagacggtct acacaaggtt tgagtctgcg ctagataaaa tcacgagggc
     3721 agaagcgggc gatgtacaaa tgtttattga gcatctacag gagaggctcg at
//
LOCUS       DASZAK010000121         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_179951, whole genome shotgun sequence.
ACCESSION   DASZAK010000121 DASZAK010000000
VERSION     DASZAK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179951"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..162
                     /locus_tag="VGT44_02085"
     CDS             10..162
                     /locus_tag="VGT44_02085"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV2579615.1"
                     /translation="MLGEDLDAASAAYRVGYHDASHFNREYKSLFGVPPMRDVQQLRE
                     AAREGY"
     gene            complement(156..1187)
                     /locus_tag="VGT44_02090"
     CDS             complement(156..1187)
                     /locus_tag="VGT44_02090"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2579616.1"
                     /translation="MEASLNGPSGNIALSAAALTIGRAPDNLLVLPDPQSSSHHAEVR
                     PDVNGYVVIDLNSTNGTFVNEQRLAPQAPRPLITGDVIRIGAARFTYEVAGSFGETLR
                     AGASEFNSNAYQPTVSASPPPASVPQYQSYPDYPAYQAPQAQPPSGYGDYGQQQGFSG
                     YQQPPVQPGTPPVSAPQGYPIPQAGFQQQWNALPGQMGAPQIPQVASAPAKKKSRTGL
                     VIGLVVLLLLLVGGGIGGYLYLRSTPDKTLQAYCNGWLSSNAQEVFDQLSTQAQTQTS
                     VSKISAIMQSANDPRVGGFKTCTFTPPQQSSTTATSTVTLTLTAAIAPPIVTHDVLID
                     ENGTWKISQ"
     gene            1489..2700
                     /gene="queG"
                     /locus_tag="VGT44_02095"
     CDS             1489..2700
                     /gene="queG"
                     /locus_tag="VGT44_02095"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEV2579617.1"
                     /translation="MLDATTIKEYAYSLGFDLARITSADAFPETERIVRERIERGLMD
                     GLPWFTAERAGVSCHPAALLPEARSIIALAMFYLAEQPDETDDGLPRGRISRYAWGDD
                     YHDIIKPKLQQFAAWLREYAQDDLPDTQTRLFVDTGRMVDRAVAQRAGLGWYGKNTNI
                     LTHGWGSWVFLAEIVTNLPLGGDTDSQYDQPSKASCGHCDICLRACPTGALPAPYVLD
                     NTRCISYLTIELRGSIPIELRPLMGNLIFGCDICQQVCPVNIVAERRLGLRHDDKVAS
                     TPLLQLQPRPAFRPRADTGSSPALIPLLSLTEEQFRERFRYSPIKRAKRRGLLRNVCV
                     ALGNIGDPQAIPALTASLRDAEALVRGHAAWALGQIGGPRARQALEGALPEEADESAR
                     QEILCALSQLN"
     gene            2956..>3238
                     /locus_tag="VGT44_02100"
     CDS             2956..>3238
                     /locus_tag="VGT44_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579618.1"
                     /translation="MKHHDRPGILLSSSAVADNAHELSVQQDSKPDKNSAIRCIALPT
                     DVSNYLNFAETSRGTTSAESPQTRSSTLTRAPGAGYKPLGYEPRLAAPVT"
BASE COUNT          680 a         1018 c          906 g          634 t
ORIGIN      
        1 cgccgtctga tgctgggcga ggacctcgac gcggccagtg cagcctatcg cgttggatac
       61 cacgacgcct cccacttcaa tcgggagtac aaaagcctct tcggcgtccc cccgatgcgc
      121 gacgtgcaac agctgcggga agccgccagg gaaggctatt gactgatctt ccacgtgccg
      181 ttttcatcga tcaacacgtc atgcgtcaca atcggtggag cgatagcggc agtgagagtt
      241 agcgtgacgg tcgaggtagc agtagtgcta ctctgctggg gaggagtaaa cgtgcaggtt
      301 ttgaaaccac cgacgcgcgg atcattggcc gattgcatga tcgcgctgat cttcgacaca
      361 ctggtctggg tctgggcctg cgttgaaagt tgatcgaaga cttcctgtgc gttactactc
      421 aaccagccat tacagtacgc ttgcaacgtt ttgtcgggcg tcgagcgcag gtaaagatac
      481 ccgccgatac cgccgcccac gagcagcagg agcagcacga ccaggccaat caccagtccg
      541 gtgcgactct tctttttagc aggcgcagac gctacctggg gaatttgcgg cgcgcccatt
      601 tgccccggca acgcattcca ctgctgctgg aagccagctt gcggtatggg atagccctgc
      661 ggagccgaaa ctggcggagt tcctggctgt accggtggct gctgataccc ggaaaagccc
      721 tgctgctgtc cataatcgcc atagcccgat ggtggctgtg cctgcggcgc ctggtaggcc
      781 gggtagtctg gatagctctg atactgcggg acactcgcag gcggcggcga cgcggaaacg
      841 gtcggttgat acgcattact gttaaattcg cttgcgcccg cgcgcaaagt ttcgccaaaa
      901 gaaccggcta cctcgtaagt gaacctggca gcgccaatgc gaatgacatc acccgtaatc
      961 agtgggcgcg gcgcctgcgg cgcaagacgc tgctcgttca caaacgttcc attagtgcta
     1021 ttcagatcaa taactacata accattcaca tcaggtcgca cctcggcatg gtgcgaagac
     1081 gactgaggat cgggcaacac cagaagatta tcgggcgctc gtccaatcgt gagggctgcc
     1141 gcagatagag caatattccc cgatggtccg ttcaaactgg cttccataaa ccaactcctt
     1201 tacaacaatg cgccaaagat taaaagtggc tcgaaagggc gaccgcgagg gtgcgccctt
     1261 accatgacca cgaggaattc cgcacgccgc tcttcgtcgg ttcatggtaa gggcgcaccc
     1321 ttgcggtcgc cctccggaat gtccccaacg gtaactttca gtgtagtgta tcatgaatac
     1381 cggcgtttgt ctgggaattt taccacattg gtactgctga attgctcaca ccccgcatga
     1441 tatactggtc actatgacga tgaattgaac aagctcacaa gatgacctat gctggacgct
     1501 acaacgataa aagaatatgc ctattcgctc ggcttcgacc tggcgcgcat taccagcgcc
     1561 gacgcctttc cagagacgga gcgcatcgtg cgcgagcgca tcgaacgcgg cttgatggat
     1621 ggcctgccct ggttcaccgc cgaacgagcc ggcgtctcct gccatcccgc cgcgcttctg
     1681 ccggaggcgc gctccatcat cgcgctggcc atgttctacc tggccgaaca gccagacgag
     1741 acggacgatg gccttccgcg cgggcgcatt tcgcgttacg cctggggcga cgattatcac
     1801 gacatcatca agccgaagct gcaacaattc gccgcgtggc tgcgcgaata cgcccaggac
     1861 gacctgccag acacacagac acgcctcttc gtcgatacgg gccgcatggt tgaccgcgca
     1921 gtcgcgcagc gagcgggcct gggctggtac gggaaaaaca cgaatatcct gactcacggc
     1981 tggggatcgt gggttttcct ggcagaaatc gtcacaaacc ttcctcttgg aggcgacact
     2041 gatagccagt acgaccagcc ttcaaaggcc agttgcggcc actgcgacat ctgcctgcgc
     2101 gcctgcccaa caggagcttt gcccgcgccc tacgtactcg ataacacgcg ctgcatctcg
     2161 tacctcacca tcgaactgcg cggcagcatc cccattgaac tgcgcccctt gatgggcaac
     2221 ttgatcttcg gctgcgacat ctgccagcag gtctgtcccg tcaacattgt ggccgagcgg
     2281 cgtctcggtc tacgccacga cgacaaggtc gcttctacgc cgctgctcca actacagccg
     2341 cgccccgctt tccgcccgcg cgccgacact ggcagcagcc ccgccttaat ccccctcctc
     2401 tcgctcaccg aggagcagtt ccgcgaacgc tttcgctaca gccccatcaa gcgtgccaaa
     2461 cgacgcggcc tgctgcgcaa cgtatgcgtc gcgctcggca acatcggcga cccgcaagcc
     2521 atcccagcct tgaccgcctc cctgcgcgac gcagaggcgc tggtgcgagg ccacgccgcc
     2581 tgggcgctcg gccagatcgg cggcccacgg gccaggcaag ccctcgaagg tgctttacca
     2641 gaagaagccg atgaatccgc gcgccaggaa attctctgcg ccttgagcca attgaattag
     2701 catctccgta ggtgccggtt caccgcgcac accgccgatt gatcggcctt gggtcttgct
     2761 cagttcgccg atggtggccg attatgaacg ttcacgatca ccatcggtca tatcccgtag
     2821 gagccgattt atcgtgctcg cagccgattg atcggcgcga atccctgtca tcttgagcgc
     2881 agcaaaggat ctcgattctt ggaacatcag acccttcgct gcgctcaggg tgacaacacg
     2941 taaccgatgg tggtcgtgaa acatcatgat cggcctggga tcttgctcag ttcgtcagcg
     3001 gtggccgata atgcacatga actgagtgtt caacaggaca gcaagccaga caaaaatagc
     3061 gcaatacgat gtattgcgct accaaccgat gtgagtaatt acctcaattt tgccgagacc
     3121 agccgaggga ccacgagcgc cgagtcacca cagacgcgtt cgtcgaccct gacccgcgct
     3181 cccggcgcgg gatacaagcc gttgggatat gagccccgct tggctgcgcc ggttaccc
//
LOCUS       DASZAK010000122         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_535384, whole genome shotgun sequence.
ACCESSION   DASZAK010000122 DASZAK010000000
VERSION     DASZAK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535384"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3694
                     /locus_tag="VGT44_02105"
     CDS             <1..>3694
                     /locus_tag="VGT44_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2579619.1"
                     /translation="KAKAPRKKATRPSKSSAPTAAAETPAEATPTAVTPGEISTPQAA
                     TPKKPQRPRKAAGTTPRAPRSRKKPSLVTRPAEAVETHEINAVVEQAETTAPEQPREA
                     VKAESREALFSDLALDEPLTETEPETPPELEETHKRHSVHLPPLLEARTTSPLPATNG
                     HNTVQEAADIVETPPTPRIDTDSVPDTPWPLAGDEDEQFVQDTLFIDAASMEMEQHNG
                     AIAAPTMPATPLPDEIDELLMLALAEVVDRDLAIIEPGEEAAPVVAPQRPQPPRAKPV
                     KARVALVLLALLALSIAALWRDVSLPHLYLYNINPLDGQANALQDLGAGYTNTTLLTN
                     PVLLRSSLLVGLPGSQRILSLNGGGQAGGLAQQINVPLTTRSQASLSIAPNQDLVVAS
                     PGGIQVLRPDGSRLWQTSAEGPSQGAHAFAPAIDTTTLYTVIAANGGEIAAYNLTSGT
                     PRWIQKLPDSFDFAPPMLVSADTLYVAGDHTLYALDSANGGPRWHVALPARTLLLDQQ
                     GARPLLIAAGASGLTALDAQSGVVAWSFSGYAGAPGSATGAGLTNAQLYQAAYSTTNQ
                     MVYTTGIVWDEQQAREQLWLFAVDASTGAPRWSEQVGSDVASADAGRVLTPFFDAAQG
                     LALLEVAQGDGSHTLSAYDTGSGSLRWSIRLTGVSAFAPTLFQTNNDTVSLLAVQSDI
                     GTALRSRSWLHLALLALGIVCLLLLLGLWFIPLSSWRLRTSTGLKRLSALSQLPGRTQ
                     QRKTRRLLFTGLLVALIAGPGSLTFLSINRPQRYLNAVGASSGSTQWQHSLDTPGALS
                     GADGQESFVVLHPSSALSALSALNSDGSLRWSTPASEGTYSLPSAQTAPGTLLVALSG
                     PTAPHYLDAPGDTGYPQLETHLFSLSLLSSATGRVLWQTTVVRGDEAQDTVVLGADAQ
                     YTYIASRAIAIPGQAAVAQLIAVDTASGAIAWRVFGPREDGTVPPDFGALLPHGRLLY
                     WQVASTIYALDTQLGQIQWRTPLAEVNATSALIEEPQMTLTAGVLLIRRSDQYHALAL
                     SSGVQLWSINGLGSPTQQAPGGMLVAANGTLILYGSNTIEALDAASRAILWQHTNLAN
                     ITGAVAAPDGSLVYAIVLDDLNNTSPTPHQALIAFDVTDSSVRWTFQPSTLALFLYPG
                     APALRLAHGMLYVTLCMAPASPPSSGDSSCNAQVLYGLNGATGAVSWQFGADQVTSVQ
                     VSQDGSAIIFQTSSSLWENFKARFLGE"
BASE COUNT          664 a         1291 c         1109 g          630 t
ORIGIN      
        1 aaagcgaagg cgccgcgcaa aaaagcaacg cgcccgtcaa aatcttcggc gccaacagcg
       61 gcagcagaaa cgccagcaga ggcgacgccc acagcagtca cgccaggtga gatttccacc
      121 ccacaggccg ctactccgaa gaaaccgcag cgccccagga aggcggcggg aacgacgccg
      181 cgcgccccgc gctcgcgcaa gaagccatcg ctcgtaaccc ggccagccga ggcagttgag
      241 acgcatgaaa tcaacgcggt cgtcgaacag gccgaaacga ccgcgccgga acagccgcgc
      301 gaggcggtca aagcggagag tcgcgaggca ctgtttagcg acctcgcgtt ggacgagccg
      361 ctcacggaaa ccgaaccgga gacgcccccg gagttggaag agacgcacaa acggcactct
      421 gtccacttgc caccactgct cgaagcgcgc actacgtctc ctctgccggc cacaaacggc
      481 cataataccg ttcaggaagc ggcagatatt gttgaaacgc cgcctacccc acgcatcgac
      541 acggatagcg tccccgatac gccctggccg ctcgccgggg acgaggacga gcaatttgtg
      601 caggacactc tttttatcga tgccgcctca atggagatgg agcagcataa cggcgctatc
      661 gctgcgccaa ccatgcccgc gactccgctc ccggatgaga tcgatgagct actgatgctt
      721 gcgctagcgg aagtggttga ccgtgacctt gcgatcatcg aaccaggcga ggaagcagcg
      781 ccggttgtcg cgcctcaacg cccccagccg ccgcgcgcga agccggtcaa agcgcgcgta
      841 gcccttgtgc tgctggcgct gctggccttg agcatcgccg cattgtggcg cgatgtcagc
      901 ctgccgcatc tttatctcta caacatcaac ccgctcgatg ggcaggcgaa cgcactgcaa
      961 gacctgggcg ctggctatac gaacaccact ctactgacca accccgtgct gctgcgttcg
     1021 tcgctgctag tagggctgcc tggctcgcag cgcatcctgt cgctgaacgg cggcggccag
     1081 gccgggggcc tggcgcagca gatcaacgtc cccctgacta cgcgctcgca ggcaagcctg
     1141 agcatcgcgc ccaaccagga cctggtggtc gcctcgcccg gcggcattca agttttgcgt
     1201 cccgatggca gccggctctg gcaaacctcg gctgaagggc cgtcgcaggg ggcgcatgcc
     1261 tttgctccgg ccatcgatac gacgacgctc tataccgtca ttgccgcgaa tggcggggaa
     1321 atcgcggctt ataacctgac cagcggcacg ccgcgctgga tacaaaagtt gcctgactcg
     1381 ttcgatttcg ccccgccgat gctggtctct gctgatacgc tctacgtcgc gggcgaccat
     1441 acgctttacg ccctcgatag cgccaacggc ggcccgcgct ggcacgtagc cctcccggcg
     1501 cgcaccctgt tactcgacca gcagggcgcg cgacccctct tgatcgcggc tggcgcaagc
     1561 ggcctgacgg cgcttgacgc gcagagcggc gtggtcgcct ggagctttag cgggtacgcg
     1621 ggcgcgcccg gctctgccac cggcgcgggc ctgaccaacg cgcaactgta ccaggctgcc
     1681 tattcgacaa caaatcaaat ggtgtacact accggcatcg tgtgggacga acagcaggcg
     1741 cgcgagcagc tctggctctt cgccgtcgat gcttcaaccg gcgcgccgcg ctggtcggag
     1801 caggtcggca gcgatgttgc cagcgccgat gctgggcgcg tcctgacccc tttcttcgat
     1861 gccgcacagg gattggcgct gctcgaagtt gcacagggcg atggcagcca tacgctctcg
     1921 gcctatgata ccggcagcgg ttcgctgcgt tggagcatcc ggctgacggg cgtctctgcc
     1981 ttcgcgccca cactcttcca gaccaataat gatactgtca gcctgcttgc cgtgcagtcg
     2041 gacatcggta ccgccctgcg cagcaggtcc tggctgcatc tcgcgctgct tgccctgggt
     2101 atcgtgtgcc ttttgctgct gctgggactc tggttcatcc ctctttcaag ctggcggctg
     2161 cgcacctcaa ccggtctgaa gagacttagc gccctatccc aattgccagg ccgcactcaa
     2221 cagcgcaaaa cgagacgcct gctcttcacc ggcttgctcg tcgcgctcat cgccggcccg
     2281 ggatcgctga ccttcctctc gatcaaccgc ccgcagcgct atctcaacgc cgtgggggcc
     2341 agcagcggaa gcacgcagtg gcagcactcg ctcgatacgc caggcgcgct ttcgggggcc
     2401 gacggccagg aaagctttgt cgtgctgcat ccgagcagcg cgctctcggc cctctccgca
     2461 ttgaatagcg acgggtcgct gcgctggtca acacccgcca gcgaggggac ctattctctg
     2521 ccgtccgcgc agaccgcgcc gggaacgctg ctggtggcgc tcagcgggcc gaccgcgccg
     2581 cattatcttg acgcgcctgg cgataccggc taccctcaat tggagactca cctcttctcg
     2641 ctctcgctgc tctccagcgc gacgggccgc gtcctctggc agacgacggt ggtacgcggc
     2701 gatgaggcgc aggacaccgt tgtgctggga gcggacgcgc agtataccta catcgccagc
     2761 cgcgcgattg ccattcctgg acaggcagcg gtcgcgcagc tcatcgccgt tgatacggcc
     2821 agtggggcga ttgcctggcg cgtctttggc ccgcgtgaag atggcaccgt gccgcccgac
     2881 ttcggcgcgc tgctgccaca cggacggctg ctctactggc aggtcgccag cacgatctac
     2941 gccctcgata cgcagcttgg gcaaatccaa tggcgcacgc cccttgccga ggtgaatgca
     3001 acctccgcgc tcatcgagga accgcaaatg acgctgactg ccggggtgct gctcatccgg
     3061 cgcagcgacc agtatcacgc gctggccctg tcgagcgggg tccagctctg gtcgatcaac
     3121 ggcctcggct cccccactca gcaggcgccc ggtgggatgc tggtggcagc caacggcacg
     3181 ctgatcctgt atggcagcaa caccatcgag gcgctcgacg cggccagccg cgccattctc
     3241 tggcaacata ccaacctggc gaatatcacc ggtgcggttg ccgcccctga cggttcgctg
     3301 gtgtatgcca tcgtgcttga cgacctgaat aacacttctc ccaccccaca ccaggcgctc
     3361 atcgctttcg atgtgaccga tagctctgtg cgctggacct tccaaccctc caccctggcg
     3421 ctcttcctct accccggcgc gcccgcgctg cgcctcgcgc acgggatgct ctacgtcacg
     3481 ctctgcatgg ctcccgcgtc accaccatcc agcggagact cctcgtgcaa tgcacaggtc
     3541 ctctacggcc tcaacggagc cacaggcgcg gtgtcctggc agttcggggc cgaccaggta
     3601 acatcggtgc aggtcagcca ggacggcagc gccatcatct tccaaaccag cagcagcctc
     3661 tgggaaaact tcaaagcgcg cttcctggga gaat
//
LOCUS       DASZAK010000123         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_109701, whole genome shotgun sequence.
ACCESSION   DASZAK010000123 DASZAK010000000
VERSION     DASZAK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109701"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1164
                     /locus_tag="VGT44_02110"
     CDS             100..1164
                     /locus_tag="VGT44_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HEV2579620.1"
                     /translation="MEHLNAVILAGGSGTRLWPLSTPAFPKQFLPLPNGRSMIQESLA
                     RVAPLVEPGHAWVVTGRSMGGLAHEHLPTVPESHILGEPIGRNTSAAIAWAAATIARE
                     DPQAIMVVVTADHVIRKVDVFNQALRLAARLASQGNMVTLGIKPTAPETGYGYIHYAE
                     PVSEGFDHEAFRAERFVEKPDLATAKQYLQAGRYVWNSGMFIWKVETILSELRQHLPE
                     LMRKIDIMVQAMNTPQERATFDELWPTLQIISIDYGVLEKTKNLVVIPVDIDWNDVGN
                     WEQYASLFDADGQGVRAVGPHNALGCQNIFVYNDTARQVFTIGLEDLIIVEMADKTLI
                     CHRDAVQRVKELAESQLKKA"
     gene            1182..>2046
                     /locus_tag="VGT44_02115"
     CDS             1182..>2046
                     /locus_tag="VGT44_02115"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEV2579621.1"
                     /translation="MLAEQPTYAAHGMVACANYLATQAGLRVLMQGGNAIDAAIAANA
                     TMTVVYPTTCSAGGDLFMLIWDAKTQRLYALNGSGRAPQGMTPDLFVAHGLASIPERG
                     PLSINVPGAVDGWFEALGRFGSLSTEAVFAPAIEYAQEGVPVTAKLARWLANSEPLLR
                     RWESTTAVFLPNNRPPRPGERLRMPDLAQTYRLLAREGRDAFYRGPIGLAITAYVQQC
                     GGVLSMDDLHRHHSDWVEPISTTYRGYEVYEFPPNSQGIAALEMLNILEGYDLASMGY
                     LSARCLHLLLEA"
BASE COUNT          417 a          632 c          607 g          390 t
ORIGIN      
        1 cgatcatact gataatgtac caggaacatt tgctggtaat gtaccaggaa catttgctgg
       61 gctatcagaa cttatgagaa ttaaaggtga cacgtttcta tggagcattt gaacgccgta
      121 atactggccg gggggagtgg gacgcgcctg tggccgctca gcacaccggc atttccgaaa
      181 cagtttttgc cgctgcccaa cgggcgcagt atgatccagg aatcactcgc gcgcgttgcg
      241 cccctcgtcg agccggggca tgcatgggtc gtgacgggtc gcagcatggg cgggttggcg
      301 cacgaacatc tgccgacggt gccggaatcg catatccttg gcgaacccat cgggcgtaat
      361 acttccgccg ccatcgcctg ggctgccgcg accatcgcgc gcgaagaccc gcaggccatc
      421 atggtcgtcg tgaccgccga ccatgtcata cgcaaagtgg acgttttcaa ccaggctctc
      481 cggctggccg cgcgccttgc tagtcagggc aacatggtga cgctgggcat caagccgacc
      541 gcgcccgaaa cgggctatgg ctacattcac tacgccgagc cggtcagcga aggtttcgac
      601 cacgaggcgt tccgcgccga gcgttttgtc gaaaagcccg acctggcaac ggccaagcaa
      661 taccttcaag ccgggcggta tgtctggaat agcggcatgt ttatctggaa ggtcgagacc
      721 atcctgtccg aactgcgcca gcacctgccc gaactcatgc gcaaaatcga catcatggtg
      781 caggccatga acacccccca ggaacgcgcc accttcgatg aactctggcc aacgttgcag
      841 attatctcaa ttgattatgg cgtgctggaa aaaacgaaga acctggtcgt gatccccgtc
      901 gatatcgatt ggaacgatgt cggcaactgg gagcaatacg cctcgctttt cgacgccgac
      961 ggacagggcg tgcgtgctgt cggcccgcac aacgcgctgg gctgccagaa catcttcgtc
     1021 tacaacgata ccgcgcgcca ggtcttcacc atcggcctcg aagacctgat tatcgtggag
     1081 atggcggata agacgctgat ctgccaccgg gacgctgtcc agcgtgtcaa agaactcgcg
     1141 gaaagccagc tcaaaaaagc ctgaacacga ggagggaacc attgctcgcc gaacaaccaa
     1201 cctatgctgc gcatggcatg gtggcctgcg ccaattatct cgcgacccag gcaggtctgc
     1261 gcgtcttgat gcagggcggc aatgccatcg atgctgcgat tgcagccaac gcgacgatga
     1321 cggttgtcta tcccacgacc tgttcggcgg gcggcgatct cttcatgctg atctgggacg
     1381 cgaagacgca gcgactctat gccctgaatg gcagcggacg cgccccgcag ggcatgacgc
     1441 ccgacctgtt cgtcgcgcac ggcctggcat ctattcccga acgcggcccg ctatcgatta
     1501 acgtgccggg cgcggtggat ggctggttcg aggcgctggg ccgcttcggt agtctttcaa
     1561 ccgaggccgt tttcgcgccc gctatcgagt acgcgcaaga aggggtgccg gtcacagcga
     1621 aactggctcg ctggctggcc aattccgaac cgctgctgcg gcgctgggag agtaccacag
     1681 cggtgttctt acccaacaat cgcccgccgc gccccggcga acgcttgcga atgccagacc
     1741 tggcgcagac ctatcgcctg ctggcgcgcg aggggcgcga tgctttctat cgcggcccaa
     1801 tcgggctggc catcaccgcc tacgtgcagc agtgcggcgg cgtactgagc atggacgatc
     1861 tgcatcggca tcattccgat tgggtagagc cgatctcgac cacctatcgc ggctacgagg
     1921 tctacgaatt cccgccaaat tcgcagggca tcgccgcgct ggagatgctt aacatcctcg
     1981 aaggctacga tctcgcctca atgggctacc tctctgccag gtgccttcac ctcttgcttg
     2041 aagcga
//
LOCUS       DASZAK010000124         3105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_535423, whole genome shotgun sequence.
ACCESSION   DASZAK010000124 DASZAK010000000
VERSION     DASZAK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535423"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VGT44_02120"
     CDS             <1..537
                     /locus_tag="VGT44_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2579622.1"
                     /translation="DLPVRGRDLVMLGLDGLRWGFSLPGAARRQEHARVAEALEAVEA
                     TAYADRPIGQLSGGEQQRLFLAQALVGQPRLLLLDEPLASLDLRSQSGIAGLVARLAR
                     ELGFTVLLVTHDVNPVLPVVDRVMYIARGQVAIGTVDQIITTQKLSSLYEAPVEVIRD
                     SLGRIFVVGLEEVEGHAR"
     gene            534..1448
                     /locus_tag="VGT44_02125"
     CDS             534..1448
                     /locus_tag="VGT44_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV2579623.1"
                     /translation="MNTSIVLLDLPTLSWNLVADWQDIFQYGFMRNAFLAGTIIAIIA
                     GTVGYFVVLRGLAFASHTLANIGFAGAAGAVLIGLQPVYGLLVFTLVGGLGMSAFGKR
                     LSQRDITVGIILSVALGLGILFISLYQGYSTDAYAVLFGEVLGISQQDVLVALVVGGF
                     TLLALGTMYRPLLFASLDEQAAEAKGAPVQLMNVAFMLVLALAVAEAVQIVGVLLIFA
                     LLVTPAAIAERLATRPPMALLLSVLLALLFTWGGLVIAYYLPYPLGFFITTLAFGTYL
                     VVRLVKDGPWRFNRGYVAAQNTSEGGAS"
     gene            1445..2401
                     /locus_tag="VGT44_02130"
     CDS             1445..2401
                     /locus_tag="VGT44_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV2579624.1"
                     /translation="MSGFVQGVLLASTQPFSWNLIADVQQMLSVDFMRSAFLSGSLIA
                     VTCGLVGYFVLLRSLTFASDALSHVGFAGAMAAAFLGLNPYVGLFGSSIIVGIGMGQL
                     GRSSARSRDVAIGTVLAWILGLGALFLGLYLAHPVANTNIAVGVGILFGSIFGVDART
                     ALVISVVGVATSIALIVMARPLLFASLDPDVARARGLPVRLLDGLFLALLAITVAEAV
                     PAVGALLVFALLVTPAASVRSIVRRPYVALVLSAALALVITWVGLTMAFYLSYPVSFD
                     ISALGFAVYLVLLAFQRAQALIREMNKIIQHPDGEALPGTKM"
     gene            2646..3074
                     /locus_tag="VGT44_02135"
     CDS             2646..3074
                     /locus_tag="VGT44_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEV2579625.1"
                     /translation="MLADKIYAAFDEVSQRNTRPRRLIAERLIALAKSGADFTIDDLW
                     HSMRAEEPRMGRATVYRAVEKLVSMGLLGRIEFADGTHHYRVCGDHHHHHVTCTRCHR
                     VEEVDICLPEDQFTAIGNLTDFAIEGHSLTLFGRCKDCRN"
BASE COUNT          542 a          871 c          972 g          720 t
ORIGIN      
        1 gacctgcccg tgcgggggcg cgacctggtg atgctggggc tggatggtct gcgctggggc
       61 ttctcgctgc caggagcggc ccggcggcag gaacacgccc gcgtcgcgga ggcgctcgaa
      121 gcggtggaag cgacagcata tgccgaccgc cccatcggac agttgtcggg gggcgagcag
      181 cagcggctat tcctggcgca ggcgctggtt gggcagccgc gcctgctgct gctcgacgag
      241 ccgctcgcca gccttgacct gcgcagccag tcgggcatcg ctggccttgt cgcccggctc
      301 gcacgcgaac tcggctttac cgtgttgctc gtgacccacg acgtgaaccc cgtcctgccc
      361 gtcgtcgacc gcgtcatgta tatcgcgcgc ggccaggtag ccatcggcac ggttgaccag
      421 atcatcacca cgcagaaact gagcagcctg tatgaggcgc cggtcgaggt gatacgcgat
      481 agtctgggcc gcatcttcgt cgtgggactt gaagaggtgg agggtcacgc gcgatgaaca
      541 cgagtatcgt cctgctcgac ttgcccaccc tctcctggaa tctggtggcc gattggcagg
      601 atatcttcca gtatggcttc atgcgtaacg cattcctggc tggcacgatc atcgccataa
      661 tagcgggtac ggtcggctat ttcgtagtgc tgcgcggcct cgcctttgcc agccacacgc
      721 tggccaacat cggtttcgcc ggagccgccg gggcggtact gattggcctc cagccggtct
      781 atggattgct ggtctttacc ctggtcggcg gtctgggtat gagcgccttt ggaaagcgac
      841 tttcccagcg cgatatcacg gtcggcatca tcctatcggt agcgctaggt ctcggcatcc
      901 tgttcatctc gctgtatcag gggtacagca cggacgctta cgcggtgctg ttcggggagg
      961 tgttgggcat cagccagcag gatgtgctgg tagcgctggt ggtgggtggc tttacgttgc
     1021 tggcacttgg gacgatgtat cgcccgcttc tgtttgcctc gctggatgag caggcagcag
     1081 aggcgaaggg cgcgccagtg caactcatga acgtggcatt catgctcgtt ctcgcgctcg
     1141 ctgtggccga ggcggtgcag atcgtgggag tcttactgat ctttgccttg ctggtaacac
     1201 cggcagcaat tgccgagcgg ctggcgacgc gcccaccaat ggcgctgttg ctctcggtat
     1261 tgctggcgct gctcttcacc tggggagggc tggtcatcgc ctactatctc ccctaccccc
     1321 tgggcttttt cattacgaca ctggcgttcg gaacatatct cgtagtgcgc ctcgttaaag
     1381 atgggccatg gagatttaat cggggttacg tggctgcgca gaacacgtcg gaaggaggag
     1441 cgtcatgagc gggtttgtac aaggtgttct gctcgcgtct acgcagccat tttcctggaa
     1501 tctgatcgcg gatgtccagc agatgctgtc ggtggatttt atgcgcagcg ccttcctgtc
     1561 cggctcgctg atcgcggtaa cgtgcgggct ggtggggtac tttgtcctgc tgcgcagcct
     1621 gacctttgcc agtgacgcgc tctcgcatgt tggctttgcc ggggcgatgg ctgccgcgtt
     1681 cctgggattg aacccctacg tgggactctt cggcagctcc atcatcgttg ggatagggat
     1741 gggacagctt gggagaagca gcgcccgctc gcgcgatgta gcgattggaa cggtgttggc
     1801 ctggattctg gggcttggcg ctttgttcct gggtctttac cttgcccatc cagttgccaa
     1861 taccaatatc gccgtcggtg ttgggatact tttcggttcg atcttcggcg tcgatgcgcg
     1921 cacagcgctg gtcatcagcg tagtaggcgt agcaaccagc atcgccctga tcgtaatggc
     1981 gcgtccgctg ctctttgcct cgctcgatcc cgatgtggcg agggcgcgcg gcctgccggt
     2041 acgtctgctg gatggactgt ttctggcgct gctggcgatc acggtggccg aggcggtgcc
     2101 ggctgtgggc gcgctgctcg tcttcgccct gctcgtcacg cccgccgcga gcgtgcgcag
     2161 catcgtaaga cggccatatg ttgcccttgt actctccgca gccctggcgc tggtgattac
     2221 ctgggttgga ctgacgatgg cattctatct gtcctatcca gtcagcttcg atatcagcgc
     2281 gctgggtttc gcggtgtacc tggtcttgct tgctttccaa cgagcgcaag cgctgataag
     2341 ggaaatgaac aaaattatcc agcatcctga tggtgaagct ctaccaggga cgaagatgtg
     2401 ataatgttca caaacaagat cggtaatcct cttgtcatcc tgagcgcagc gaaggatctc
     2461 ggtggtcggc gcatccgatc cttcgctgcg ctcaggatga cagaaccgac tttagtcgca
     2521 aatgttcatt atcgtcgctt tagctcaact ggtttagctg aacaagcgtc gaatatggta
     2581 aacttggaag tagccaccat cggcacactc aatgccgaga aatgcccaga aatggtgagg
     2641 tcaaggtgtt agcagacaaa atttatgcag cgttcgatga agtgagccag cgcaacacac
     2701 gtccacggcg cttgatcgca gaacggctga tcgcgctggc gaaaagtggc gctgatttta
     2761 cgattgatga cctctggcac tccatgcgcg cggaggaacc tcgtatggga cgagcaacag
     2821 tctaccgcgc ggtagaaaag cttgtcagca tgggcttgct gggtcggatc gagtttgctg
     2881 atggaacgca tcattatcgt gtctgcggag atcaccacca ccatcatgtc acctgcactc
     2941 gctgtcaccg cgttgaagaa gtggatatct gcctgccaga agatcagttc accgctattg
     3001 gaaatctgac ggattttgcc atcgaaggac actcgctaac cctctttggc cggtgtaaag
     3061 actgccggaa ttagcgtgcc tcaggctgat cttgcataga aagct
//
LOCUS       DASZAK010000125         3226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_571056, whole genome shotgun sequence.
ACCESSION   DASZAK010000125 DASZAK010000000
VERSION     DASZAK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571056"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..503)
                     /locus_tag="VGT44_02140"
     CDS             complement(48..503)
                     /locus_tag="VGT44_02140"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV2579626.1"
                     /translation="MDISGNQKVRANRQQVFQALLDPAVLKSSVPGCESAEFVEFPTG
                     RQLKLVVSPNLPGFKGPYNIFLQTGEVIAPSRVVMIAEPTSSFGSVKATCAIDLADDA
                     EGTQLSYKAEAEVSGKIAGVPDIAINTAVKMGLGQFFKNFEKQVSAARV"
     gene            complement(701..2938)
                     /locus_tag="VGT44_02145"
     CDS             complement(701..2938)
                     /locus_tag="VGT44_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2579627.1"
                     /translation="MDSLIGTSVGGYTLVSVLGSGGMGTVYLAEDPAIGQQVAIKVVR
                     TDPDSLTDIGALSLSAERFKQEARAVASLDHLHILPLYRYGEEKMSGGQRAYIIMQYR
                     HEGSLWDWLRRRAEVTFSQAGNLPGATGGAAASILPNGVWPLPLAEANEYLEQAASAL
                     QYAHDRGIIHRDVKPANFLLRFENGKPVHLLLSDFGLAKLFSSNSATSNILGTPLYMA
                     PEQFEGAVIPESDQYALAVMIYYLLAGRPPFEGEPMRLMNLHLSTPVPPIESINPALP
                     ASVGQVLARALAKRPADRYASIADFAAAFAQAAQEQPGAIAGLRPLLSLPTLAQGAPV
                     SPYAPTVVHPPAATPQATPQQLPIQPLQPISGGSFPTANTEIGLQPTRQAFAPAESPA
                     WATYSPSIPSTPMPPTPAQKQGMSRRGALGWILGGAAAAAIAGGAGLFIYSKLHTPAH
                     SLSVLQGHSAPVTSVSWSLDGSLLASASRDKTAKVWQASNGQLVTTYSRHTAAILSIA
                     WGQGPGGPLLASGGEDRTVQVWNTSAVRYRLFPRLAAPVSSLAWSLDSSYILAGTQGG
                     GAHALLLSNGTITSSIGRLNIHALAFSPDGSYIALALQSGFVTVVTVQEPHKTVFSTG
                     QASPALCLAWSPDGSRLAVGFANNAAEVYNFSPGSSTNRSILRSLPHNGAVNGIAWEP
                     SNGATPRLATAAGDGTLNIWDMADSAATTYNGYGPAMLSVAWSSGGLATGDANNTVIL
                     WQVVE"
BASE COUNT          607 a          899 c         1043 g          677 t
ORIGIN      
        1 gcggcaggag gagatgctct gctgcaccgc cgcgctggtc gtgttgatta gactctcgct
       61 gctgagacct gcttctcgaa attcttgaag aattggccca ggcccatctt cacggcggtg
      121 ttgatggcga tatctggaac accggcgatt ttgccggata cttcggcttc ggctttgtag
      181 gacaactgcg taccttccgc gtcatcggca aggtcgatgg cgcaggtggc cttgacggag
      241 ccgaaggagc tggtcggctc ggcgatcatg acgacgcgcg agggtgcaat cacctcgccg
      301 gtctgcaaga aaatgttgta cggccctttg aagccgggca gattgggcga aacgaccagc
      361 ttgagctggc gacctgttgg gaattccaca aattccgcgc tctcacagcc ggggacgctg
      421 ctcttcagaa ccgcggggtc caacaatgct tgaaatacct gttgccggtt agccctgacc
      481 ttctggtttc cagaaatgtc cattgtacgt ttttcctctg ttctctagtc tccataccat
      541 atgatagatg atggtaagca tatgcaggta tgggatatat ggggttaacg tgtggtagta
      601 tttgcccgtt tatcatgaaa taccgcccag gcactcacac accttacata gaattataga
      661 cgttgatcta tcgttagctc aagtattagc acaagaagta ctattccact acctgccata
      721 ggatgaccgt gttgttggcg tcgcctgtgg ccaggccccc gctgctccag gcgacggaaa
      781 gcatggctgg cccatagccg ttgtaggttg tggccgcgct atctgccatg tcccagatgt
      841 tgagcgtacc atcgccagca gccgttgcca ggcggggtgt tgcgccattg gagggttccc
      901 aggcgatgcc gttcaccgcg ccgttatggg gtaagctgcg caggatactc ctgttggttg
      961 agctaccggg gctgaaattg tatacctcgg cggcgttgtt ggcgaagccg accgccagtc
     1021 tgctgccatc cggcgaccag gccaggcaga gcgctggtga cgcctggccg gtggaaaaca
     1081 ccgtcttgtg tggctcttgc acagtgacga cggtcacgaa accgctttgg agcgccagag
     1141 cgatgtagct gccatcgggt gagaaggcca gggcatggat attgaggcgg ccaatactac
     1201 tggtgattgt gccgttgctc agtagcaggg cgtgagcgcc gccgccctgc gtgccggcca
     1261 ggatgtagct gctgtcgagg ctccatgcta gcgagctgac cggtgcggcc aggcgtggga
     1321 agaggcgata gcgcactgcc gaggtattcc agacctgcac ggttctgtct tcgcctccag
     1381 aggcgagcag tggtccgccg ggaccctgac cccatgctat gctcagaatc gcggcggtat
     1441 gcctgctata cgtcgtcacc agctgcccat tggatgcctg ccagactttc gccgttttat
     1501 cgcgcgaggc ggaagcgagc agcgagccat cgagcgacca ggaaacactg gtcacgggag
     1561 cgctatgccc ctgcagaacg ctcagggaat gagcgggcgt gtgaagtttc gagtaaatga
     1621 acaggcccgc gccgcctgcg atggccgctg ctgccgcgcc gccgagaatc cagcccagcg
     1681 cgccgcgcct gctcatgccc tgcttctgag cgggcgtcgg cggcattgga gtggagggta
     1741 tggatgggga atacgttgcc cacgcgggag attccgctgg agcaaaagct tgccgggttg
     1801 gttgaaggcc aatctctgtg ttggctgtcg ggaatgagcc gccggaaatg ggttgtagcg
     1861 gctgaatggg cagctgctgc ggcgttgctt gcggtgtggc tgccggtgga tgcacgactg
     1921 tgggggcgta gggtgatacc ggcgcgccct gcgccagggt tggcagcgac aggagcggtc
     1981 gcaggccggc gatggctcct ggctgttcct gggctgcctg cgcgaaggct gcggcgaaat
     2041 cggcaatgct ggcgtagcgg tcggcggggc gtttggccag cgcgcgcgcc agcacctgtc
     2101 cgacgctagc gggcagagcg gggttgatgc tctcgatcgg cggaacgggc gtggatagat
     2161 gcaggttcat caggcgcatc ggttcgccct cgaatggtgg ccgcccggcc agcaggtagt
     2221 agatcatgac cgccagggcg tactggtcgc tttctgggat gaccgcgccc tcgaactgct
     2281 caggggccat gtagagcggc gtaccgagaa tgttgctggt ggcggagttg gaagagaaga
     2341 gctttgccag gccgaagtcg gaaagcagca gatgaaccgg cttgccattt tcaaagcgca
     2401 gcaagaagtt ggcaggcttg acatcgcggt gaatgatgcc ccggtcgtgc gcgtattgca
     2461 gtgcggaggc agcctgctcc aggtactcgt tcgcctcggc gagcggcagc ggccagacgc
     2521 catttggcag gattgaggcg gcagcgccgc cggtcgcgcc gggcaaatta cccgcctggc
     2581 tgaatgtcac ttctgccctg cgcctcagcc agtcccacag cgagccttcg tgccggtact
     2641 gcatgatgat ataggcgcgc tgacccccgc tcatcttctc ctcgccatag cgatacagcg
     2701 gcaggatgtg caggtgatcg aggctggcga cggctcgcgc ctcctgctta aaacgctcgg
     2761 cggatagcga taaagcgccg atgtcggtca ggctgtcggg atcggtgcgc acgaccttga
     2821 tggcgacctg ctgtccgatg gcagggtctt ccgccaggta caccgttccc atgccgccgc
     2881 tgccaagcac agaaacgagc gtgtatccgc caacggacgt gccgatgaga ctatccacgt
     2941 taatcctgcc tccgtctctc aagttcgccg tgtatacatt ggactctaga catcaattgt
     3001 ctcggaaata cgctccgagg ctcttctacc gcgcaccgcg catattctag catactcctc
     3061 tacgctcgca caatgcgcat agctctattg gcatatccat ctcatacagc ctgggacggg
     3121 gtttgaggag gtgtttcaac ctatgtcacc gtgactttcg gatttggcga ggttgagaga
     3181 atcttgagga atgaagcatg atatgaagta tcgacaaata gagagc
//
LOCUS       DASZAK010000126         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_251598, whole genome shotgun sequence.
ACCESSION   DASZAK010000126 DASZAK010000000
VERSION     DASZAK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251598"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VGT44_02150"
     CDS             complement(<1..218)
                     /locus_tag="VGT44_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579628.1"
                     /translation="MSQSAAPNRHPDVARAEQTMPALLDDLETVVNIDSGTYTKAGID
                     RVGTYLQELFQTFGFSTSFDAQTEYGNHL"
     gene            complement(215..1405)
                     /locus_tag="VGT44_02155"
     CDS             complement(215..1405)
                     /locus_tag="VGT44_02155"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEV2579629.1"
                     /translation="MTQPLSGIRILDLSRLLPGAYATQMLADFGADVIKVEEPGTGDY
                     GRFMPPHSVGGMSLYFLAINRNKRSMTLNLKSAEGREVFLRLVRTADVVLESFRPGVM
                     ERLGLGYEQLKEKHPELIYCAISGYGQDGPYRLRAGHDLNYAGYAGLLHYNRGPHGEP
                     AMPASQFGDLAGGSFMAVMGILAALAGRAQTGVGGMVDVSMTEGVMALLPLTSAAYLN
                     TGTPPRPGRNSLDGGLPCYNIYETSDGKHVTLAALEYKFWHTFCTHIGHLELLPFYTP
                     VGPGEREQAIEMLQAIFKTRTRDEWMAELADIDACVGPVYTIEEAMNDPQAQARGVSI
                     TSRLADENGASFQTIPSFPRISGVEQEQRHAVPGLGEHTAALLQELGYEEAEIERMRS
                     GGVL"
BASE COUNT          320 a          481 c          440 g          334 t
ORIGIN      
        1 agatgattgc catactcggt ctgcgcgtcg aacgaggtgg agaagccgaa cgtttgaaat
       61 agttcctgga gataagtccc cacacgatca atgcccgctt tggtgtacgt gccggagtcg
      121 atattcacaa ccgtctcaag atcatcgagc agagcaggca tggtctgttc tgctcgcgcc
      181 acgtctggat gcctgtttgg agcggcggac tggctcatag caccccccct gagcgcattc
      241 gctcgatctc agcctcctcg tagcccaact cttgcagcag ggcagctgtg tgctcgccca
      301 ggccgggtac ggcatggcgc tgctcctgct ctacgccgga gatgcgcggg aaggatggga
      361 tcgtctggaa gctcgccccg ttctcatcag cgagccggct ggtaatgctg acaccgcgtg
      421 cctgggcctg gggatcgttc atggcttcct cgatagtgta gacggggccg acgcaggcgt
      481 cgatatcggc cagttccgcc atccactcgt cgcgcgtcct ggtcttgaag atggcttgca
      541 gcatctcgat ggcttgctcg cgctcccccg gcccgaccgg cgtgtaaaac ggtagcaatt
      601 ccaggtgtcc gatgtgtgtg cagaaggtgt gccagaactt gtattccagc gcggcgagcg
      661 tcacatgctt gccgtccgat gtttcgtaaa tattataaca gggcagcccg ccatccaggg
      721 agtttctacc gggccgagga ggtgtgccgg tattgagata cgcggcgctt gtgagcggca
      781 gcagcgccat caccccctct gtcatagaga catccaccat gccgccgacg cccgtctgcg
      841 ctcggcccgc cagcgcagcc agaatgccca tcaccgccat gaagctgccg ccggccaggt
      901 cgccgaactg cgacgcgggc atcgccggtt caccgtgtgg tccccggtta taatgcagca
      961 gaccggcata tcccgcgtaa ttcaggtcat gccccgcgcg cagccgatag ggtccatcct
     1021 ggccatagcc gctaatcgcg cagtagatca gctccggatg tttctccttg agttgctcgt
     1081 atcccagacc caatcgctcc atcacacccg gacgaaagct ttctagcacc acgtcagccg
     1141 tgcgaaccag gcgcaagaac acctcacgcc cctcggcaga cttgaggttc agcgtcatgc
     1201 tgcgcttgtt gcggttgatc gccaggaaat acaggctcat cccgcccacg ctgtgtggtg
     1261 gcataaaacg cccatagtct cccgtgccag gctcctcgac cttgatcaca tccgcgccaa
     1321 aatcagccag catctgcgtc gcataagccc ctggtagcaa gcgtgaaagg tcaaggatac
     1381 gaataccact gagcggctgg gtcatgctct ctgcctttct atcattgaag gtgaaacgag
     1441 atcattttat taaaaggtaa caggcatgaa cgggtgttgt caatgtggtg aagtgataca
     1501 ggtgtggcta tttaatacct gcttatttgc cacaagtctt tcctacaccc aggtgataca
     1561 atgcctaaaa acaac
//
LOCUS       DASZAK010000127         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_109751, whole genome shotgun sequence.
ACCESSION   DASZAK010000127 DASZAK010000000
VERSION     DASZAK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109751"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VGT44_02160"
     CDS             complement(<1..375)
                     /locus_tag="VGT44_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2579630.1"
                     /translation="MSSSSPVPTSQQGQTYQNFIGGEWRSARRGETFASTNPARTSEV
                     IGYYQKSGAADIDDAVAAALKAQPGWAATPAPERGEVLYRTAAILERRKEELATLMTR
                     EMGKILRETRGDVQIAIDVAKFI"
     gene            complement(484..939)
                     /locus_tag="VGT44_02165"
     CDS             complement(484..939)
                     /locus_tag="VGT44_02165"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HEV2579631.1"
                     /translation="MTVVLGLLLSVVFALLLNSPRLLLQHSAVSGLFGVVMLLSLLFP
                     RPVTFYLIRSIKTQNDPRQLADFNASWSSPQVRSFYRVLTLVWGCVTIAQVVVTAFLS
                     FSLSIPVMLVISPFLGFAFILPVAHWSMGYFRKNKTMFEQLRQQREASF"
     gene            complement(969..1193)
                     /locus_tag="VGT44_02170"
     CDS             complement(969..1193)
                     /locus_tag="VGT44_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579632.1"
                     /translation="MATTTSTLTQNDTKQSYVSQNNGGRGMMSRQMIPNLLINAAAPF
                     AINMLAQQYISTIDALLLASSVQPCTPWVV"
     gene            1309..1416
                     /locus_tag="VGT44_02175"
     CDS             1309..1416
                     /locus_tag="VGT44_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2579633.1"
                     /translation="MSDATVKTHINHIFSKLGARDRSQVVIKARQFGLA"
     gene            complement(1540..2115)
                     /locus_tag="VGT44_02180"
     CDS             complement(1540..2115)
                     /locus_tag="VGT44_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579634.1"
                     /translation="MVPQELTNFFTVSAGVGATLIGLIFVAVSIAPERTVMEGAPLER
                     QAVATSAFSALVNAFFISLIALIPGSGFVITTLTMSIIGLSGISLPAWSLLKHPGGWR
                     AFIRNFTALLVSVVLYVYELFQAIALLQQSADSAAVYTLAVLISVAYALGLLRSWQLL
                     GARRFRLSNLLVPSKDAGAQKSDAEQAVGGT"
     gene            complement(2123..2881)
                     /locus_tag="VGT44_02185"
     CDS             complement(2123..2881)
                     /locus_tag="VGT44_02185"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2579635.1"
                     /translation="MADIIDAHVHIGRYHLPIEEIDSLLKGSGISRAVVFADPESGDM
                     VNDSAYALEAARRVGYYAFYYYGGNAYSGQRPYDRLPVPDTLDQYNGIKWHCWFSPAH
                     DGGLRYSYPIDMDNVRRQMDAPDFQAVMEAIRTLNFPMTFEEHFEVTREFVSRYPDVI
                     LIIPHMGMLNGGQERVQAEFRDFPNVHFDTSLGSIDEKIVQTLGASRLLYGCDYPYGW
                     PADNLRRVQRLQIPDEEKEMMLGGNVLRLIRRKE"
     gene            complement(2973..3353)
                     /locus_tag="VGT44_02190"
     CDS             complement(2973..3353)
                     /locus_tag="VGT44_02190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV2579636.1"
                     /translation="MSQFTSEEIEYLKSQHLGRLATVNKAGEPHVVPVTFRYNEELDT
                     IDIGGHGIGKTKKFRDIAQNANVAFVVDDVLPPWRPRSVEIRGRAALHSEGGQTIMPD
                     FSPELIRIVPRRVLSWGLGDSTAS"
     gene            complement(3438..3731)
                     /locus_tag="VGT44_02195"
     CDS             complement(3438..3731)
                     /locus_tag="VGT44_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579637.1"
                     /translation="MAQWAYKVAYIDYRGRISSEGLETLIGNNERRSTFARRYLNSLG
                     KDNWELVSVQPLTGNSAYYVFKRPAQEGDFAEEAPSASGESAPAAGGPTIETA"
BASE COUNT          874 a         1105 c         1012 g          827 t
ORIGIN      
        1 aataaacttc gctacgtcaa tcgcaatctg cacatcgcca cgtgtctcgc gcaaaatctt
       61 gcccatttcg cgcgtcatca gggtcgccag ttcctctttg cgccgctcca ggatggcagc
      121 agtgcgatac aaaacctcgc cgcgctccgg cgcgggcgtg gccgcccagc ccggctgcgc
      181 cttcaacgcc gccgcgaccg cgtcatcaat atcggctgcg cccgatttct gatagtagcc
      241 gatcacctcc gacgtgcgcg ccgggttcgt gctggcgaac gtctcgcctc tacgcgcgct
      301 gcgccattcg cctccaatga aattttgata cgtttgaccc tgctgcgacg ttgggacagg
      361 ggatgaacta gacatgggta tgctcctccg agaactccga ggtctaaatt gtacaggtgc
      421 attatatcac ccaccaacat cagaggaggc aaggattctg aaagggtcgc ccttcggaaa
      481 cctctaaaag cttgcctcgc gctgctggcg cagctgctcg aacatcgtct tgttcttgcg
      541 gaagtagccc atgctccaat gagcgacggg gaggatgaag gcgaagccca ggaaggggct
      601 gatgaccagc atcaccggga tcgacaggct gaaggacagg aacgccgtta ccaccacctg
      661 cgcaatcgtt acacagcccc agacaagcgt caggacacgg tagaacgagc gcacctgcgg
      721 ggatgaccag ctggcgttaa aatcggcaag ttgccgagga tcattttgcg tcttgataga
      781 gcggatcaga tagaacgtga ccgggcgagg gaagagcagc gacagcagca tcactacccc
      841 gaacaggccg ctgaccgccg agtgttgcag cagcaggcgc gggctattga gcagtaaggc
      901 gaagaccacg ctcaatagca gccccagcac aacggtcaca cccagcagat cgatgcgcct
      961 tttccagatc agaccaccca gggcgtacag ggctggacag aactcgccaa cagcagcgcg
     1021 tcgatagtgg atatgtactg ctgagccagc atattgatcg cgaagggtgc tgcggcattg
     1081 atcaacaggt tcgggatcat ctggcgagac atcatgccgc gcccgccatt attttgactg
     1141 acatacgatt gcttcgtatc gttctgtgtc agagtagaag tggtggttgc cattgttgtt
     1201 tccctttctt gttgcctgtt tgattatcga tgtttgattt ctttttcctg gaaccgctcg
     1261 tttcatttac ctttccgtgc ggctcgtcgc ttacatgcgg aattgtttgt gagcgacgcg
     1321 accgtcaaaa cgcatatcaa ccacatcttc tccaaactcg gcgcgcgcga ccgttcacag
     1381 gtcgtcatca aagcgcgcca gtttggtttg gcctgactac gtcaccggtc gtaagaacgc
     1441 gatgatggcg attgataaat taatccggcc accagagtag agagagcgag ggacttacta
     1501 tacggctaca ttacatattc aatgtgcata atcgcgtccc tatgtccccc cgaccgcctg
     1561 ttccgcatcg cttttttgcg caccggcatc cttcgacgga acgagcaagt tgctcagcct
     1621 gaatcgtcgt gcgcccagca gttgccagga gcgcaagagg cccagcgcgt atgcaacgct
     1681 gatcagcact gccagcgtat agacggcggc tgaatcggca gattgctgta gaagcgcaat
     1741 tgcctggaag agttcataga cataaagaac gacactgacg aggagagcgg tgaaattgcg
     1801 gataaaagcc cgccatcctc caggatgttt cagcagactc caagcaggca atgaaatacc
     1861 tgacagcccg atgatactca tggtaagcgt ggttatgaca aagcccgaac ccgggataag
     1921 agcgatcaat gagatgaaaa aggcattgac cagggcagaa aaggcgctgg tagccactgc
     1981 ctggcgttcc agtggagcgc cttccatcac cgtccgttcg ggtgcgatgg aaacagcaac
     2041 gaagatcagg ccgatcaatg ttgccccaac cccggcgctc acggtaaaga aattagtcaa
     2101 ttcttgtgga accatgcaga acttattcct ttcttcgtat caaccgcagc acattcccgc
     2161 ccaacatcat ctccttctcc tcgtccggga tttgcaggcg ctgcacacgc cgcaggttgt
     2221 cagccggcca gccataggga tagtcgcagc cgtagagcag gcgcgatgcc cccagcgttt
     2281 gcacaatttt ttcgtcgatg ctccccaggc tggtgtcgaa atgaacattc ggaaaatcgc
     2341 gaaactccgc ctgaacgcgc tcctggccgc cattgagcat ccccatatgc ggaataatca
     2401 gtatcacatc gggataacgg ctcacaaatt cgcgggtcac ttcaaaatgt tcctcgaagg
     2461 tcatggggaa attgagggtg cggatagcct ccatcacggc ctggaaatcg ggcgcgtcca
     2521 tctgccggcg tacattgtcc atatcgatcg ggtagctgta gcgcagccca ccatcatgcg
     2581 cgggtgagaa ccagcagtgc catttgatgc cattgtactg gtccagcgta tccggcaccg
     2641 gcaggcggtc atatggtcgc tgcccgctat acgcattgcc gccataataa taaaaggcat
     2701 aatagcccac acgccgcgcc gcttcgagcg cgtaggccga gtcgttgacc atgtcgccgc
     2761 tctccgggtc ggcgaaaaca accgcccggc tgataccgct ccccttcagc agcgagtcga
     2821 tttcttcgat cggaagatgg tatcttccaa tatggacatg cgcgtcgata atatctgcca
     2881 tgtgtgcctc ctctcatgcc ataccaaagt gtagcacaaa attatggcga aaaggcaggg
     2941 cgaccgccag cacatgtcct tcgtaacttt tgctatgatg ctgtactgtc gccaagcccc
     3001 caggatagga cgcgcctggg gacaatccgt atcagttccg gggaaaaatc cggcataatc
     3061 gtctgcccac cttctgaatg caaagccgcc cttccgcgta tctcaacgga gcgtggtcgc
     3121 caggggggaa gcacatcatc aacgacgaac gcaacatttg cattctgcgc gatatctcgg
     3181 aacttcttgg tcttgcctat accatggccg ccgatatcaa tggtatccag ttcctcgtta
     3241 tagcgaaatg tcactggcac aacatgcggc tcgcccgcct tattcacggt tgccagcctg
     3301 cccagatgct ggctcttcaa atattcgatc tcttccgatg taaattgact cataaacaac
     3361 tcctcaccat atccagatgc gtaaagggta gtggaaacac ttgcaagcgt ttccactacc
     3421 ctttacgcat catcagtcta tgccgtctcg atggtcggac cgccagcggc aggcgcggac
     3481 tcgccggaag cagatggcgc ttcctcggcg aaatcgccct cctgcgcggg ccgcttgaag
     3541 acgtagtagg cggaattgcc ggtcaacggc tgaacgctga ccagttccca gttatccttg
     3601 cccagggagt tgaggtagcg gcgtgcgaag gtgctgcggc gctcattgtt cccgatcaag
     3661 gtctccagcc cttcgctgga aatacgtcct cgataatcga tatacgcgac tttatacgcc
     3721 cattgtgcca tgtctgcttt cggctccttt cacatctgga atgtttctat caacaatact
     3781 actcgattgt ctcgcttcaa tgggcgtgta ggggggca
//
LOCUS       DASZAK010000128         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_180062, whole genome shotgun sequence.
ACCESSION   DASZAK010000128 DASZAK010000000
VERSION     DASZAK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180062"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VGT44_02200"
     CDS             complement(<1..978)
                     /locus_tag="VGT44_02200"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF003652.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEV2579638.1"
                     /translation="MYKEIINPLLGKLDSENWHVRAREALHLAEATPFTLKLLELFAD
                     QHRRFSDERLHVVLGGVAFDNPVMVGAGWDKAGRAVKALYTLGFAGVEVGSVLAYAQD
                     GNPRPRQFMPGPGVALNRLGFNTPGMEVVAHNLERYQGSGIPIGISLGKNRDVDAEDA
                     PEAHAAVARKLYDDAAYFAINVSSPNTPGLRELQDKASLTQIVQAVNQAMDEKGARKP
                     LFVKIAPELTDEAVDDVIQVVVDNKLTGIIATNSTINQEIKAKYGEKWRNEAGGVSGD
                     DVDYRRMATAQVAYIWRETRGSIDIIGVGGVKDAQTALEKIKAGAKVVQV"
     gene            1085..1948
                     /locus_tag="VGT44_02205"
     CDS             1085..1948
                     /locus_tag="VGT44_02205"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEV2579639.1"
                     /translation="MKHTSFLYKALVLVVVIVPLLAIPLAIGLLWQREVRWSDIILLA
                     SMYTLVALGVTVGFHRMLTHRSFRPSPVIKFILLVLGSMSLEGPALEWAATHIKHHAQ
                     SDREGDPHSPVDGFFHAHIGWLFSNDKADPNVYCRNLVNDRMVVFVSRTFALWAVLTL
                     VIPFAIGGWSGLLWGGLVRIFLTHHVTWSVNSVCHTFGKREFETNDQSHNEWVVGLLG
                     FGEGWHNNHHAFPRSAFHGLHWWQFDLSGYLIWSLERIGLARDVYRIPAALRARRVIG
                     QAALPPKDEVA"
     gene            complement(1989..>3044)
                     /locus_tag="VGT44_02210"
     CDS             complement(1989..>3044)
                     /locus_tag="VGT44_02210"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEV2579640.1"
                     /translation="CPTVGISGLTLGGGVGVLSRKYGLTCDNLLSAQVITANGNLLNC
                     GANDNANLFWALRGGGGGNFGVVTSFVFQLHAVAALTLFTLDWPWSVAANVVDAWQNW
                     ILPAPDELWSNCLLLSTNDTSATPIVRVNGVYVGSAGPLDALLSQLIKRVGSVPTGNY
                     VFTDSLLETMMVEAGCYGESVAACHLPSQNPQGQLSRDTSGAKSDYFTNLLPRQGIDA
                     LIHEISRRQSSSLLGAGGIGLDSFGGAINRVAPDATAFVHRNALFSAQYTANWMAGAQ
                     DSVIAANYSWLNGLWQMMRPSASGAAYQNYIDPDLSDWQKAYYGANLPRLQQIKASYD
                     PGDLFHFAQSIPLAGAS"
BASE COUNT          634 a          918 c          774 g          718 t
ORIGIN      
        1 aacctgcact acctttgccc ccgccttgat tttctccaac gccgtctgcg catcctttac
       61 gcctcccacg ccgataatat cgatgctccc ccgcgtctcc cgccagatat atgccacctg
      121 tgccgttgcc attcttcgat aatccacatc atcaccactt actccgcctg cttcattgcg
      181 ccatttttcc ccatactttg ctttgatctc ctggttgatg gtactattcg tcgctatgat
      241 acccgtcagc ttgttatcta ctactacctg aatgacatca tcgaccgctt cgtccgtcaa
      301 ctctggcgca atcttcacga atagcggctt tcttgctccc ttctcatcca tcgcctgatt
      361 taccgcttga acgatctgcg tcagcgatgc cttatcttgg agctcgcgca gtccaggcgt
      421 attcggcgat gaaacattga tagcaaaata ggccgcgtca tcatacaatt tcctggcaac
      481 cgccgcgtga gcttctggag catcttcagc atcgacatct ctattcttgc ccaggctgat
      541 cccaatcgga ataccactcc cttgatatct ctccaggtta tgagcgacaa cctccatgcc
      601 aggcgtatta aagcccaatc ggttcaacgc cacgccggga ccaggcataa actgtcgtgg
      661 cctgggattc ccgtcctgag cataggccag cacggagcca acctcgacgc ccgcaaaccc
      721 caacgtatac aacgccttca cagcgcgtcc tgctttatcc catccagcgc caaccatgac
      781 cgggttatca aacgccaccc cacccagcac tacatgcagc ctctcatcgc taaatctacg
      841 gtgttgatca gcaaacagtt ccagcagttt caacgtaaac ggagtcgctt cggcgagatg
      901 gagcgcttcc ctggctctga catgccagtt ttccgaatct agtttcccta gtagcgggtt
      961 aataatttct ttatacatat ttcaagcctt tccctcattt caccggaccg tgaaacaaac
     1021 acaaggaaat ggcactctaa cagtcacccc gcttttctgg cggggcatta gtttggaggg
     1081 ttttttgaaa cacacttctt tcctctacaa agcccttgtg cttgtagttg tgattgtacc
     1141 attgctggct atcccgcttg ccatcggttt gctctggcag agggaagttc gctggtcgga
     1201 catcattctg ttagcctcga tgtatacgct ggtggctctt ggcgtgaccg tcgggttcca
     1261 tcgcatgttg acacaccgca gtttccggcc aagcccggtc atcaagttca tcctactcgt
     1321 actcggctcg atgtcgctgg aaggacctgc gcttgagtgg gctgctacgc atattaaaca
     1381 ccacgcgcaa tctgatcgcg agggggatcc ccatagcccc gtcgatggct ttttccatgc
     1441 gcacatcggc tggctattca gtaacgacaa ggccgatccg aatgtgtatt gccgcaacct
     1501 ggtcaatgac cgcatggtcg tgtttgttag ccgcactttc gcactatggg ccgtattaac
     1561 gctggtcatt ccttttgcca tcggtggttg gagcggtctg ctgtggggcg ggctggtacg
     1621 cattttcctc acccaccacg tcacctggag cgtcaattcg gtctgccaca cctttggcaa
     1681 gcgcgaattt gagacgaacg atcagagcca taacgaatgg gtcgttggcc tgctcggttt
     1741 tggcgagggc tggcataaca atcatcacgc cttcccacgt tccgcgtttc acggcctgca
     1801 ctggtggcaa ttcgatctct ccggctacct gatctggtcg ctcgagcgca tcggacttgc
     1861 tcgtgacgtg tatcgcatcc cggcagcctt acgcgcgcgt cgtgtcattg ggcaggcagc
     1921 cttgccccca aaagatgaag ttgcttaaca tcagttagac caggtggcac agaggtccct
     1981 tttctttttc aggatgcgcc cgcaagtggg atgctttgcg cgaaatggaa gagatcgccg
     2041 ggatcatagc tcgccttgat ctgctgcaag cgtggcagat tggctccgta gtaggccttc
     2101 tgccagtcgc tcaaatccgg gtcgatatag ttctggtagg cagcgccgct cgcggatgga
     2161 cgcatcatct gccacaaccc gttcagccac gagtagttcg cggcaataac ggagtcttga
     2221 gcgcccgcca tccaattcgc cgtgtattga gcggagaaga gcgcgtttcg gtggacaaag
     2281 gcggttgcgt cgggggcaac acggttgatc gcgccgccaa aggagtcgag tccgataccg
     2341 ccggcgccca gcagagaaga ggactggcgg cggctgatct catgtattag agcatcgatc
     2401 ccctggcgcg gcaaaagatt ggtaaaataa tcggacttcg cgccagatgt gtcgcgcgac
     2461 aactggccct gcggattctg gctgggcaag tgacacgcgg caacactttc accataacaa
     2521 cccgcctcaa ccatcatggt ctcgagcagg ctatcagtaa acacatagtt gccggtcggg
     2581 acgctgccaa cacgtttgat taactggctg agcagtgcgt cgagcggccc ggcgctgcca
     2641 acgtagacgc cattgacgcg cacgattggc gtagcactcg tgtcgttggt agacagcagt
     2701 aagcagtttg accacagctc gtccggcgct ggcaatatcc agttttgcca ggcatcgacc
     2761 acgttggcgg caacgctcca cggccagtcc agcgtaaaca gcgtcagcgc cgcgaccgcg
     2821 tgtaactgga agacgaacga tgtgacgacg ccgaagttac caccgccgcc gccgcgtagc
     2881 gcccagaaga ggttggcatt atcgtttgcc ccacagttga gtagattacc attagccgtg
     2941 atgacctgcg ccgaaagcag gttatcgcaa gtgaggccat acttgcggct gagaacgcca
     3001 acaccaccgc cgagcgtcag gccgctgata cccacagttg ggca
//
LOCUS       DASZAK010000129         4244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_606722, whole genome shotgun sequence.
ACCESSION   DASZAK010000129 DASZAK010000000
VERSION     DASZAK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4244
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606722"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            322..1521
                     /locus_tag="VGT44_02215"
     CDS             322..1521
                     /locus_tag="VGT44_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease domain-containing protein"
                     /protein_id="HEV2579641.1"
                     /translation="MEKTDAELVALARTGNRDAFGCLIERYTQMVRRLTASMIADESI
                     AREMAQEAMVQAYLSLDHLRDSARFKSWLYGITLNVCRSYLREQQASVVSLENLVGGM
                     NDGGFDHAALREGAIDPQALVEERELRLHVLQAVQRLSPKERAVTLLFYYEQYTLQEI
                     ANLLALSVTAVKGRLHRARKQLRAQLASWYEVLPQERKRKRHMRRATIESVREHPETR
                     QHVVVLKDAEDRELVIWIGKSEAWAIAGGLANFAQPRPMTAQLMVNLLQATGSTLEEV
                     RIDALKDEVFFATLKVRGANGEQELDARPSDALTLATLVNCPIFVADEVMQRVAQSDA
                     SERWLRPASEFRVIDRAALEQEQREREAAMQQTITKVGELSARQHKQEQEQAKQERIA
                     DWKETRP"
     gene            complement(1524..2447)
                     /locus_tag="VGT44_02220"
     CDS             complement(1524..2447)
                     /locus_tag="VGT44_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEV2579642.1"
                     /translation="MQQLLPYLQISEEVQAALQEQRPVVALESTVIAHGLPYPGNLEV
                     AQAMELAIRNEGAVPATIGILNGHIVIGLSREQIERLGTKGTDQHVLKVSRRDLAVAL
                     ATRQLGATTVAGTMLCASMAGIRFFATGGIGGVHRGAETSMDISADLTELARTTVLVV
                     CAGAKSILDLDLTLEYLETQGVPVLGWQTDEFPAFYVRSSGRRLPHRVDDIATVAAIA
                     RAQWECGLHGLVVGCPIPPQYALERGPLESATEEALRLARQEGIRGSATTPFLLAHVA
                     SATRGESVEANRALLVNNATWAARFAGAYYR"
     gene            complement(2448..2606)
                     /locus_tag="VGT44_02225"
     CDS             complement(2448..2606)
                     /locus_tag="VGT44_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579643.1"
                     /translation="MVLLENLPWLLFPIYIIYRMWRYPTPFSRPGEVVGNLVPVEQKS
                     VKTGVEGA"
     gene            2909..3874
                     /locus_tag="VGT44_02230"
     CDS             2909..3874
                     /locus_tag="VGT44_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2579644.1"
                     /translation="MKFGKLLTTAVVVGGTLGALTLLNKMTESQAGELDTVLKGEERR
                     YPWKNGDVFYLVKGNRESKPLLLVHGLGPGASSYEWRKNIDALSEQFRVYALDLMGYG
                     LSDRPAIDYTPETYADLVNDFLKEVINKPTVVVAHGETCAYVIANAYRRPQLFERLVL
                     VAPPLSIVEETVPGPVNAALKFLLRAPIVGQFAYNILTSRRGIQNYYDREGYHNPGLI
                     TDELVEYIFSSAHQLNARYAAASALTKNLVMDAHEPLVRLQVPVVAIVGRESIPTPAE
                     VSAALKRVNSRIETRILDNCSSQPQDEQAAKFNALVREFAAAPVA"
     gene            4038..>4244
                     /locus_tag="VGT44_02235"
     CDS             4038..>4244
                     /locus_tag="VGT44_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579645.1"
                     /translation="MRSVEDQMTTLTARDREPRASVTIADVWEAHKLLKSYLHHTPLA
                     PSRMLREATGADIYLKAENMQRSGS"
BASE COUNT          912 a         1227 c         1257 g          848 t
ORIGIN      
        1 ctcgccccta cacgtcaaat ataccaggcg cgctatagat agtataccgg gcagagcgaa
       61 tagtacgaag ttgggtgatg tttattacac gaattgcata gttcatagga cgctattata
      121 tggtatgaat ttcgaggttg aggtagggaa cgtaccaggg tgtgacaatt gtctcatgag
      181 gaggttaatg caataatgag ttgatgataa cagtctaata aacgcatttg taagttgaat
      241 ttaactactc atatacaaat acaacctttt tcgttaaaat tgctactact ataacagagt
      301 catacattcc cgcaggacga catggagaag acggatgccg aactggttgc cctggcgcgc
      361 actggcaacc gggacgcctt tggctgctta atcgaacgct atacccagat ggtcagacgg
      421 ctgacggcca gcatgatcgc cgatgagtcc atcgctcgcg agatggcgca ggaggcgatg
      481 gtgcaggcat atctgtcgct tgaccacctg cgggattccg cgcgtttcaa aagctggttg
      541 tatggcatca cactgaatgt ctgccgcagt tacttgcgcg aacagcaggc gagtgtcgtt
      601 tcgctggaaa acctggtggg cggcatgaat gatggcggct tcgaccatgc cgctctgcgc
      661 gaaggcgcta tcgatcctca ggcgcttgtg gaagagcgcg agttacgcct gcacgtcttg
      721 caagcggtgc agcgtctctc gcccaaagag cgcgcggtca cgctgctctt ttactatgag
      781 caatatacat tgcaagagat cgcaaacttg ctggccctgt ccgtcacggc ggtcaaggga
      841 cgcctgcacc gcgccagaaa gcagctgcgc gcacaacttg catcatggta cgaagtgcta
      901 cctcaagaaa gaaagagaaa gagacacatg agacgcgcga caattgagag cgtcagggaa
      961 caccccgaaa cgcggcagca tgtagtggtg ttaaaagacg cggaagaccg ggagttggtg
     1021 atctggatcg gcaaatccga ggcctgggca atcgctggcg gcctggccaa cttcgcgcag
     1081 ccgcgaccta tgactgccca gctgatggtc aatctcttgc aagcaaccgg ttcgacactg
     1141 gaagaggtgc gtatcgatgc gctcaaagac gaagtctttt tcgcaacgct gaaagttcgc
     1201 ggcgcgaacg gcgagcagga actggatgcg cggccaagcg acgcgctcac cctggccacg
     1261 ctggtgaact gccccatctt cgtcgcggac gaggtgatgc agcgtgtagc gcaaagcgat
     1321 gcctcggaac gctggctgcg cccggccagc gagttccgtg tgatcgaccg cgcagctctg
     1381 gaacaagagc agcgggagcg cgaagctgca atgcagcaga ccatcaccaa agtaggcgag
     1441 ctatccgcgc gccagcacaa gcaggagcaa gagcaggcca aacaggaacg catcgctgat
     1501 tggaaagaga cgcgcccgta gcgttagcga taatacgcac ccgcgaaccg cgccgcccac
     1561 gtcgcgttgt tgaccagtag cgctctattc gcctcgacgc tttcgccgcg ggtggcgctg
     1621 gcgacatgcg ccagtaggaa cggcgtggtg gccgaaccgc gaatgccttc ctgccgcgcc
     1681 agccgcaggg cttcctccgt cgcggactcc agcgggccgc gttccagggc gtattgcggc
     1741 gggataggac agccgacgac caggccgtgc aggccgcact cccactgcgc gcgcgcgatg
     1801 gcggcaacgg tggctatgtc atctacgcgg tggggcaggc gcctgccgct ggaccggacg
     1861 tagaaggcag ggaattcatc ggtttgccag cccagcacag gtacgccctg cgtctcaagg
     1921 tattccagcg tcaggtcaag atcgaggatg gacttcgctc cggcgcagac caccagcacg
     1981 gtcgtgcgcg ccagctcggt caggtcggcg gagatgtcca tgctggtttc cgcgccgcga
     2041 tgcacgccgc cgatgccgcc ggtggcgaag aagcgaatac cggccatact cgcgcacaac
     2101 atcgttcccg cgacggtcgt cgcgcccaat tgccgcgtcg ccagcgcgac ggccaggtcg
     2161 cgcctgctga ccttcaagac atgctggtct gttccctttg ttcccagccg ctcgatctgc
     2221 tcgcgcgaca ggccgatcac gatatggcca ttcaggatgc cgatggtggc cggaacagcc
     2281 ccctcattgc ggatggccaa ttccatcgcc tgcgccacct cgagattgcc aggatagggc
     2341 aggccatgcg cgatgacggt cgattcgagc gcaacgaccg ggcgctgctc ttgcagggct
     2401 gcctggactt cttcggagat ctgtaagtag ggtaaaagtt gttgcattta tgctccttcg
     2461 acgccagtct tgacgctctt ctgctcgact ggaaccaggt tccccacaac ctcgccaggt
     2521 cggctaaatg gagtcgggta gcgccacatg cgatagatga tgtagatagg gaagagcagc
     2581 cagggcaggt tttccagcag caccatgcca ggttgtggcg tgccaatctc tgcgaaagtg
     2641 ggatggtatg tctcttcaag gctatgctcc aggagttcta gcagagaatg ttacaaacgc
     2701 tgtttgccag attatacgtg gtaccagcgt actttgtcga atttgcgccc ccatcctgct
     2761 acatgatata ttactctttg ggctaaactc tgccccagat cgatagctga cattacaggc
     2821 agatttggta tccaggacca gaatagagat ggatgcctgg cacagttttg tgcaattgat
     2881 tcacgcattg gaatgaggag aagcacacgt gaaatttggg aaactcttga cgactgcggt
     2941 ggttgttggt ggaacgcttg gagccttgac cctgttgaat aaaatgaccg agtctcaggc
     3001 gggcgagctt gacacggtct tgaaggggga ggagcgtcga tacccgtgga aaaatgggga
     3061 tgtattttac ctggtgaaag ggaatcgcga gtcgaagccg ttattgctgg tgcatggcct
     3121 cggccccggc gcgtcttcat acgaatggcg caagaatatc gacgcgcttt ctgagcagtt
     3181 ccgcgtctac gcgctcgacc tgatgggcta tggcctctca gatcggcccg ccattgacta
     3241 cacgccggag acctacgccg acctggtgaa tgacttcctg aaggaagtta tcaataagcc
     3301 gaccgtcgtg gtggcgcatg gcgagacctg cgcgtatgtg attgccaacg cctatcgccg
     3361 cccacagctc ttcgagcgcc tggtgctggt cgctcctccg ttgtccatcg tggaggagac
     3421 cgttcccggc ccggtcaacg ccgcgctcaa gttcctgctg cgcgcgccca tcgtgggcca
     3481 gtttgcctac aatatcctga cctcgcgacg cggtattcag aactactacg accgcgaagg
     3541 ctatcacaat cccggcctga ttactgatga actggtcgag tacattttca gcagcgcgca
     3601 ccagctcaac gcgcgctacg ctgccgcctc cgcattgacg aagaacctgg tgatggatgc
     3661 gcacgagccg ctggtgcgcc tgcaagtgcc ggtcgtcgcc atcgttggcc gcgaatccat
     3721 cccgacaccc gccgaggtca gcgctgccct caagcgggtc aactcacgca ttgagacgcg
     3781 catcctcgac aactgcagca gccagccgca ggatgagcag gcagcgaagt tcaacgccct
     3841 ggtgcgcgag ttcgccgccg cgccggtggc gtaacattac ctgcgtttgt ggcatgtcaa
     3901 ccctcgcggt cgccatgcca caaacatcct cttgcctccc cttgcaacct gcgctataat
     3961 tgcccataca ctactacccg ctcgccacaa cgttcgtggc acagtccgat gtcgcactga
     4021 aaggaccatg ctgcgctatg cgttctgtgg aagaccagat gactaccctg accgcgcggg
     4081 atcgtgagcc gcgcgcgtct gtcacgattg ccgatgtgtg ggaagcgcac aagctgctca
     4141 agtcgtacct gcatcacacg ccgttggcac cttcgcgcat gttgcgcgag gcgaccggcg
     4201 cggatattta cctgaaagcc gagaatatgc agcgctccgg ttcc
//
LOCUS       DASZAK010000130         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_322656, whole genome shotgun sequence.
ACCESSION   DASZAK010000130 DASZAK010000000
VERSION     DASZAK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322656"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VGT44_02240"
     CDS             complement(<1..298)
                     /locus_tag="VGT44_02240"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2579646.1"
                     /translation="MQSMKEFNWREFYKHTSGRPPWPRLMRAVTHVSRRENALDLGCG
                     AGRDTLYLLEQGFAVTAVDADAHSMAILATFPHQEHLRAVQSTFVDFAFAPTGYD"
     gene            429..>1712
                     /locus_tag="VGT44_02245"
     CDS             429..>1712
                     /locus_tag="VGT44_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2579647.1"
                     /translation="MSVHTQASETGSPAASLDILALYRTMVTARVTNDLLKTRKTQGR
                     FPFYIGCAGHEGMAAVAAALEPTDWLSLYYRDLAVWLQRTSDIYGPLREAYSRTTGPM
                     GAGRNMPSHYSSKRLRILPTMSEVGALAPFAGGVAFSLKRHNSNDIIVFTIGDGGAAT
                     NDFNVLLRQATVHRLPILIVVEDNGWAITTASPIQWGGSLVETAKGAGIYAEEVDGAD
                     VMTTYDASKRLVERVRSGREPVLMHLRLGLLDSHSSSTDIKAYRTKEEIEATAATKDP
                     VKNFSRWMIEHDLLDEADIERIRKEVRAELERAEAQILQEPEPTAERVMRKVVEVPQW
                     QENIPRGAKRQLPMLGAINEALVELAKRDDGFFVYGQDVASPKGGVFGATAHLVTQLP
                     GRAINSPLNEQLIVGIVAGSGMVDGKARCAEIQFVD"
BASE COUNT          377 a          507 c          506 g          322 t
ORIGIN      
        1 tatcgtatcc agtcggcgca aaggcgaaat caacgaaagt agattgcacg gcgcgcagat
       61 gctcctgatg gggaaacgtc gccaggatcg ccatcgaatg cgcatccgca tcaaccgccg
      121 tcaccgcaaa tccctgttcg agcagataga gcgtatcgcg acccgcgccg cagcccaggt
      181 caagcgcatt ctccctgcgc gacacgtgcg tcacggcgcg cattagcctg ggccagggcg
      241 gcctgccgct ggtatgtttg tagaactcac gccagttgaa ctctttcata gattgcatta
      301 taacatgccc ctttgctaac gcatatattg cgtttagtgg ctcgtgattc tataatggtg
      361 acggcacagg ccaaaactac aaatcaacga tccgatccag tcagtcaatg aagacttatg
      421 gagggtatat gtccgttcac actcaagcat cggaaaccgg ttcgccagca gcgtcgctgg
      481 acattctcgc actgtatcgc acgatggtca cggcgcgtgt gaccaatgat cttttgaaaa
      541 ctcgtaaaac acaggggcgc ttcccgttct atatcgggtg cgctgggcat gaaggcatgg
      601 ctgcggtcgc tgccgcgctc gaaccgaccg attggttgtc gctttactat cgcgacctgg
      661 cggtgtggct gcagcgtacc agcgacatct atggtccatt gcgcgaagcc tattcgcgca
      721 ctaccgggcc gatgggcgcg ggccgcaata tgccatcgca ctacagcagc aaacgcctgc
      781 gcattctccc caccatgagc gaagtcggcg cgctggctcc cttcgccggc ggggtcgcct
      841 tctcgctcaa acgccacaac agcaacgaca tcatcgtgtt caccatcggc gatggcgggg
      901 cagccacgaa cgacttcaac gtgctgctca ggcaggccac cgtccacagg ctgcccatct
      961 tgatcgtcgt cgaggataac ggctgggcga tcaccaccgc ttcgcctatt caatggggcg
     1021 gttcgctggt cgagacggca aagggcgcgg gcatctatgc cgaggaagtc gatggggccg
     1081 atgtcatgac cacctacgac gccagcaaaa gactggtgga gcgtgtacgg tcagggcgcg
     1141 agccggtatt gatgcacctg cggctgggac tgctcgactc gcattcaagt agcaccgata
     1201 tcaaagccta tcgcacgaag gaagaaatcg aagcgacggc tgccacgaaa gacccggtca
     1261 agaattttag ccgttggatg atcgagcatg acttactcga cgaggctgac atcgaacgca
     1321 tccgcaaaga ggtacgcgcc gagctggagc gcgcggaggc gcagatcttg caggaacctg
     1381 agccgaccgc ggagcgcgtc atgcggaagg tcgtcgaggt tccgcaatgg caggaaaata
     1441 tcccacgtgg cgcaaaacgg cagttgccca tgctcggcgc gatcaacgag gcactcgtcg
     1501 aactggcgaa gcgtgacgat ggtttcttcg tctacggcca ggatgtcgcc agccccaaag
     1561 gcggcgtctt cggagcgacc gctcaccttg tgacgcagtt gccgggccgc gccatcaata
     1621 gccccctgaa cgaacagctc atcgtcggca tcgtggcggg atcgggcatg gtggacggca
     1681 aagcgcgctg cgccgagatt caatttgtag ac
//
LOCUS       DASZAK010000131         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_109921, whole genome shotgun sequence.
ACCESSION   DASZAK010000131 DASZAK010000000
VERSION     DASZAK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109921"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1647)
                     /locus_tag="VGT44_02250"
     CDS             complement(<1..1647)
                     /locus_tag="VGT44_02250"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV2579648.1"
                     /translation="MSEQEQPGRQTSCLEESQVMAWLDGALSQAAADGVKAHLATCAR
                     CAALEWSMAMERRQIVALLSSIDLPPSAAAEPVVALAHFRERLALLAEGTDRSANAPQ
                     VDDPFLPENPSLTGPAELERGTLLPIALPGVPPRRPRVWLQTLVAAVIIVVLLGPVLL
                     VRTGLLSIGNHPSTSQTAASLKLSTSQVQFTKDSSAPQQVQLTISNFKSNDRVLLTRD
                     SQEAIQTVPGAAIVSQNPAIVNVDANGSATATIIVTTNWGYGSHTIYAEDIHSQFTAS
                     ASLLVIGATPSQPAHLMITSGVGSSTPLTTFALGPDIQGSITPKTIYLKNSSQSGSIT
                     WSASSNQSWLLISPDAGTFNQSQTIVVAVQRGNLHPGSYNNGVIKFYSNVDAVQSVTV
                     TMAVTSPPPGTSVMALSPAVLSFVTTDGVNTNLSPARPCQTLTISNPGSQPLYWSLTT
                     TSSSGEGTSGSWLTTSPSSGSIPVSSYQLVSVCAQSAGLLPGAYLGALQMSAPGAADS
                     LQTVSISLTVQPHCGLVTTSGFLTFTSVQGKASAGSQGLGL"
     gene            complement(1613..2206)
                     /locus_tag="VGT44_02255"
     CDS             complement(1613..2206)
                     /locus_tag="VGT44_02255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2579649.1"
                     /translation="MPVLVESSEAMSTEEHGRVSTSSTEEEAFESIFLRYYPLVYRLA
                     YHYVGQPDEAEDIAQEVFLRFYHLPPHASHEAQQRAWLCRVAINLGLNVVRKRKSRLD
                     QEQPLDAAMLSSADEQDPERLVLAEEQAALVRDILASLPERQQVCLGLRSVGLSYEAI
                     SEATGIPLASIGSVLVRAGQAFRRNYHERTRATRTTN"
BASE COUNT          517 a          716 c          775 g          490 t
ORIGIN      
        1 cagacccagt ccctgcgaac cggcgctggc tttgccctgc accgatgtga aggtcaggaa
       61 gccagaggtg gtcaccaggc cgcagtgcgg ctgtacggtc agcgaaatgc tgacggtctg
      121 taagctgtcg gcggctcccg gcgcgctcat ttgcagcgcg cccaggtacg cgcctggcag
      181 caggccggcg ctctgcgcac agaccgagac cagttgatag gacgagacgg gaatactgcc
      241 cgatgatgga gaggtggtca gccagcttcc tgatgtccct tcgccggacg aggaagtcgt
      301 cgtcagcgac cagtacagcg gctgcgagcc gggattgctg atggtcagcg tctggcaggg
      361 gcgagcggga gacagattgg tattcacacc atccgtggtc acaaatgaca acaccgccgg
      421 actcaaggcc atcactgatg tcccaggagg cggagaggtg acagccatgg tcacggtgac
      481 tgattgcact gcgtcaacgt ttgagtaaaa cttgatgacg ccattattat agctcccagg
      541 gtggaggttg ccgcgctgca cggccacgac aatcgtctga ctctgattga acgtgcccgc
      601 atcaggcgag atcagcagcc aggattgatt gctgctggcc gaccaggtga tggagccgct
      661 ctggctgcta tttttcaaat aaatcgtctt gggcgtgatt gacccttgaa tatctggtcc
      721 aagagcaaaa gtggtcaagg gagtcgatga ccctacgccc gatgtaatca tcagatgcgc
      781 aggttggctt ggggtagcgc cgatcaccag gagactggcg ctggcagtaa attgcgagtg
      841 aatatcctcg gcgtagatgg tatgggagcc atacccccag ttcgtcgtaa cgataatggt
      901 ggcggtcgcg gagccgttcg catccacgtt gacgatcgcc ggattctgcg agacaatcgc
      961 cgcgcccggc acggtctgga tagcttcctg gctgtcgcgg gtcagcaaga cacgatcatt
     1021 actcttgaaa ttcgagatcg taagctggac ctgctgtggt gcagacgagt ctttcgtaaa
     1081 ttggacctga ctggtcgaaa gcttcaatga tgccgccgtc tgacttgtgg atgggtgatt
     1141 gccgattgac agtaacccgg tccgcacaag gagcaccggg ccgagcagta cgacgataat
     1201 cacggcggca accagcgttt ggagccacac ccgcggacgc cgaggcggta cgccaggcag
     1261 cgcgatgggg aggagcgtac ctcgttcaag ttctgccggt ccggtaagag acgggttttc
     1321 tggaagaaag ggatcatcta cttgtggtgc gttcgcagac cggtcggtac cctcggcgag
     1381 gagcgcgagg cgttcccgaa aatgggcgag ggcgacgacc ggctcggcag cggcagaggg
     1441 cgggaggtca atgctggaga gcagcgcgac gatctggcgg cgttccattg ccatcgacca
     1501 ttccagggcg gcgcagcggg cgcaggtggc caggtgggcc ttcacgccat ctgccgccgc
     1561 ctgggagagc gcgccatcca gccacgccat gacctggctt tcctccaggc agctagtttg
     1621 tcgtccgggt tgctcttgtt cgctcatgat agtttctccg aaatgcctgc ccggcccgca
     1681 cgaggaccga gccgatagac gcgaggggaa taccggtcgc ctccgatatt gcttcataac
     1741 tcaagcccac actgcgcagt cccaggcaga cttgctgccg ctcaggtaga ctcgccagaa
     1801 tatcccgcac cagcgccgct tgctcctcgg ccagcaccag ccgctcaggg tcctgctcat
     1861 cggcgctcga cagcatggcc gcgtcgagcg gctgctcctg gtcaaggcgg cttttgcgct
     1921 tgcgcacgac attcaggccg aggttgatgg cgacccggca gagccaggcg cgctgctgcg
     1981 cctcatgcga ggcgtgtggc ggcaggtgat aaaagcgcag aaagacctcc tgcgcgatat
     2041 cctcggcttc atcgggctga cccacgtagt gatacgccag ccggtagacg aggggatagt
     2101 agcgcagaaa gatcgactca aaggcttcct cttcggttga agaggtactt actcttccat
     2161 gctcttcagt tgacatcgct tcgctgctct ccaccagaac tggcatagtg cgtgccagcc
     2221 cctttccact gtttgcactt tacgtatctc taagacacat caggatgtga ttttatgcca
     2281 ttaaacggct ccaatctgaa gaaagtcctg atacaccctc ccttttcacc tacgaaatgg
     2341 aagtacagca acgaagacac agactcttct tccaggtagc ccggccatcg acgccatccc
     2401 gcttgcgagc tgcgaaggca atggcatcac ggccgacctg cgtggagtgc aacggcagga
     2461 tggaaacgag caggcatgtg atatcggcgc gtatgagt
//
LOCUS       DASZAK010000132         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_606828, whole genome shotgun sequence.
ACCESSION   DASZAK010000132 DASZAK010000000
VERSION     DASZAK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606828"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VGT44_02260"
     CDS             <1..887
                     /locus_tag="VGT44_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2579650.1"
                     /translation="YIVETLTHPKKYKNRIETYKFSPFELELPAEEVLFAPESGDYQV
                     NGWFIPYPGATTTVVVCPGYRSDKADVLGISAILWKAGHNILAFEYHGHGQEVGTPIT
                     LGYHEIHDFLGALAYAKARTPGTRIGVIAYSMGAAVAIMGVARSSDVEALVADSAFAT
                     HAGVVDFQFRRIFHLPSPPVYWMTDNLLWLRAGYHFNQVEPIREVARIAPRPLLIIHG
                     LQDSIVNPQDATLLYNAAQEPKELWLLSNADHCGAYFDDRPAYVKKVTEFFDIHLRHV
                     PRLHLVQADGQEPFQEAS"
     gene            complement(890..1801)
                     /locus_tag="VGT44_02265"
     CDS             complement(890..1801)
                     /locus_tag="VGT44_02265"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV2579651.1"
                     /translation="MIDLCLLGTCGMMPMPGRWLSCALVRCGSSLVLLDCGEGTQVPW
                     KALGWGFRQLGAICFTHMHADHVLGLPGVLFMAAHAGRTEPLDIYGPPGTGYVVEGLR
                     RVVPELPFPVYVHELRGGEGFDLPGDLRGSCGQAAHNVPCLAYRLELKRAPVFLPEQA
                     RALGLPVHYWSRLQRGETIEYNGQAVTPERVLGASRRGISLAFITDTRPTRALGEFAH
                     DVDLLVCESMYDDPVDLPLAQAHAHMLAEESAGIAAAAGAHSLVLTHFSPKIYDTSRA
                     EKAARRIFANTRAAHDGMVMTLGYQDE"
     gene            complement(2428..4170)
                     /locus_tag="VGT44_02270"
     CDS             complement(2428..4170)
                     /locus_tag="VGT44_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579652.1"
                     /translation="MHRQSRRIPRRSPGKHGRLSPLMLIGLSVPFVLALIATAAFVLP
                     RIGSHAATTNMDCTILVPSNPLSAQGLATPYQLTATDAANGPCNEANPNQSAFVQGVI
                     YDPATGAFSVYSPLVIDAGTQPAIAPTAPTLPQGAVVALWFGFNGNNLKLQGATRRTL
                     NEARCVNGLPGSIFGQFSYCNADAFYRAANRGIASGKVQVPALQMGNDGLPCPTVRDF
                     GIVDQDPSDNVQTQYLANANGQTAQLTAANQTQLANATTLGNPSDNALVTRVLDPVLG
                     CTPWTAPNLADNNAAVAALPLDELMAAADQQAPIALVPINDPMTLNNGNPSLQKTNLY
                     RRGTDQPLAFNQNQAGGAAYCRKMIQGGMARLQKDQALFTNAASPMADVANSLFTFLA
                     NRLQQSFVNLGCQHLLNIANPVTVQTDGNGVVTSATFGTGGAAGTATATTTATVSATT
                     TVTATATNTTGNNGGVQQTAQGNAFFALDANGQNVAVELHVSYPNHSNMSVQAQVRTD
                     SCTGNIVASNAINLDNQGAAPFNTVVQNVQATALPANWFFVVTDSAQQNAVVGCGIVK
                     ANGTLGRARLGVLN"
     gene            complement(4558..>4916)
                     /locus_tag="VGT44_02275"
     CDS             complement(4558..>4916)
                     /locus_tag="VGT44_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579653.1"
                     /translation="HSRKRRWHNYHRIGISFKYSVESEAIYMNTWTIYPSLQDNRHFA
                     LGSPGGRELRRGEAVDILLGGFRIPGYIEYDPKGDYFLSRTGNGVCGLYAGMKIYLTG
                     RRGERKIVAPPVGTSA"
BASE COUNT         1094 a         1369 c         1389 g         1064 t
ORIGIN      
        1 actatatcgt tgaaaccttg acacacccca aaaaatataa aaaccgtatt gaaacctaca
       61 agtttagccc cttcgagtta gaactgcctg ccgaagaagt gctttttgcg cctgagagtg
      121 gtgactacca ggtgaacggc tggtttattc cgtatcccgg cgcgacaacg acagttgtgg
      181 tctgtcccgg ctatcgctca gacaaagcgg acgtgctggg tatctcagcg attctgtgga
      241 aagccggtca caatatcctc gcgtttgaat atcatgggca tgggcaggaa gtgggaacac
      301 ctatcacgct gggctatcac gagatccacg attttcttgg cgcgctggcc tacgccaaag
      361 cgcgcacgcc tggaacgcgc atcggagtga tcgcctattc gatgggcgcg gcagtggcta
      421 ttatgggagt ggcgcgcagc agcgatgtcg aggcgctggt ggcggatagc gccttcgcga
      481 cccacgccgg cgtagtcgac ttccagtttc gccgcatctt tcacctgccc tctccgccgg
      541 tgtactggat gacggataac ctgctctggc tgcgcgcggg ctaccacttc aaccaggtcg
      601 agccgatacg agaagtcgcc aggattgcgc cccggcctct gctgattatt catggcctgc
      661 aggatagcat tgtgaacccg caggacgcca cgttgctcta caacgcggca caggagccga
      721 aggaactctg gctgctgtcc aacgccgacc actgcggcgc atactttgac gaccgcccgg
      781 cttatgtcaa aaaggtgacg gaattcttcg atatccattt aaggcacgtt cccaggttgc
      841 atcttgtcca ggccgatggg caggaaccat tccaggaagc gtcataaaat cattcgtcct
      901 ggtatcccag ggtcatgacc atgccatcat gagcggcccg cgtattggca aagatgcgcc
      961 gggccgcttt ttctgcgcgc gaggtgtcgt agattttggg actgaagtgg gtcaggacga
     1021 gcgaatgcgc cccggctgcc gcagcgatgc cggcggattc ctcggcaagc atatgggcgt
     1081 gcgcctgcgc caggggcaga tcgacgggat cgtcgtacat gctttcgcag accagcagat
     1141 caacgtcatg ggcgaactct cccagcgcgc gtgtcgggcg tgtatcggtg atgaaagcga
     1201 gcgatatgcc acgccgggac gcaccaagca cgcgctcagg cgtgacagcc tggccgttgt
     1261 attctatagt ttcgccgcgc tgcagtcgcg accagtagtg gactggcagg ccaagcgcgc
     1321 gcgcctgttc tggcaaaaag acgggggcgc gtttcagttc gaggcgatac gcaaggcacg
     1381 ggacattgtg cgcggcctgc ccgcaagaac cgcgtaggtc accgggcaga tcgaagccct
     1441 cgccgccgcg aagctcgtgg acgtacaccg ggaaaggcag ttccggtacg acgcgcctca
     1501 acccctcaac gacatagccc gtccctggtg gaccataaat atccagcggc tccgtgcgac
     1561 cagcgtgggc cgccatgaag aggacgcccg gcaagcccag cacatggtcg gcgtgcatat
     1621 gcgtgaagca gatcgcgcca agctgccgga acccccagcc cagcgccttc cagggaacct
     1681 gcgtcccctc gccacagtcg agcaggacaa gcgacgaccc gcagcgcacc agcgcgcacg
     1741 agagccagcg ccccggcatc ggcatcatgc cacacgttcc taacaggcag agatcaatca
     1801 cgatagtatc ctcattacag attattttga tatgcctggg actatcttag cacacatctg
     1861 ccaccaacca tatgctacaa tcatttacgg gtgtaggacc gagttgcagg caaaccacca
     1921 ggcagaaatg gagccaaacc ggctcctaca gcgggggttc gatatttcat ggcacataca
     1981 cgcatgtata gaatatgaga aggagccaga tgaacacaca aatcggtata cttcaatcag
     2041 tgcaggtagg gaggccagaa cgccatgttg atcataatga aaactcaaca agagggccga
     2101 aatatgcctg ggaaacttcg tttttccgcg tacctggtcc ccaaccacgt tggctctata
     2161 ctacccatct cgaaggcaat gcgcaggccg ataccgccaa tcatggcagg ctcgaccagg
     2221 ctgtgctgtt gtatgcagcc gcccattatc tcatctggca aacagagttg agacatgctg
     2281 agataggacc tggcggattt ggcgaaaacc tgacgatcgc tggattatgc gaggaagaga
     2341 tcaaagtaat caggggtgac tgttgccagt cacccctggt ttctatctac acaggaaaca
     2401 gagtagagga tgtttcctgg aacatagtca gttcaacaca cccagcctgg cacggcctaa
     2461 cgtgccgttg gctttgacga tgccgcatcc tacgaccgcg ttttgctgag ccgagtcggt
     2521 cacgacaaag aaccagttgg ccggtaatgc cgttgcctgg acgttctgaa caactgtgtt
     2581 gaacggggct gcgccctggt tatccaggtt gatggcattg ctggcgacga tattacccgt
     2641 acagctgtca gttcgcacct gcgcctggac actcatattt gagtgattgg gatagctgac
     2701 atgtaactcc accgcgacat tctgtccatt cgcatccagc gcgaagaacg cattgccctg
     2761 agccgtctgc tgcacaccgc cattgttgcc cgttgtattt gtagcggtgg cagtcacagt
     2821 ggtcgtagcg ctgacggtag cagttgttgt agcggtggca gtgccagccg cgcccccggt
     2881 gccgaacgtg gcggaggtca cgacaccatt cccgtctgtc tgcacagtga ccgggttggc
     2941 tatattcagc agatgctggc aacccaggtt gacgaacgac tgctgcaaac gattcgccag
     3001 gaaggtgaag agcgaattgg caacgtcggc catgggcgat gccgcgttgg taaacaacgc
     3061 ctggtccttc tgtaaacgcg ccatgccccc ctgaatcatt ttccgacaat acgcggcgcc
     3121 accggcctgg ttttgattga acgcgagcgg ctgatcggtc ccacgccggt agagattggt
     3181 cttttgcagg ctcggatttc cattattgag cgtcatcggg tcgttgatcg gtaccaatgc
     3241 aatcggtgct tgctggtctg ctgctgccat cagttcatcc agcgggaggg cggcgacagc
     3301 ggcattatta tcggccaggt tcggcgcggt ccagggtgtg cagccaagca ctggatcgag
     3361 gacacgtgtc accagcgcgt tatcactcgg attgcctaac gtggtcgcgt ttgcaagctg
     3421 ggtttgattg gctgccgtca actgcgctgt ctgcccattg gcattcgcca ggtactgcgt
     3481 ctggacattg tcgctggggt cctggtccac gatcccgaaa tcgcgcacgg tcggacaggg
     3541 caaaccatcg ttacccattt gcagagccgg aacttgaacc ttgccggaag caatgccacg
     3601 gttggccgct ctatagaaag catctgcatt acaataggag aactggccga agatggagcc
     3661 gggcaagccg ttgacacagc gtgcctcgtt caaggtcctt ctcgtagcgc cctggagctt
     3721 caggttgttg ccgttaaagc cgaaccagag cgctacaacg gcgccttgcg gcagcgtggg
     3781 agcagtcggc gctatggcgg gctgcgtacc ggcgtcaata accagcggac tatacacgct
     3841 aaaggcgcct gtggcgggat cgtaaatcac gccctgcacg aacgcactct ggttcgggtt
     3901 cgcctcgttg caaggtccgt tcgccgcgtc ggtcgcggtc aactggtaag gtgttgccaa
     3961 cccctgcgcc gagagtgggt tggatggaac gaggatcgtg cagtccatat tcgtagtagc
     4021 cgcgtgtgat ccaatgcgag gcagcacaaa tgcggcggtt gcaataagag ccagcacaaa
     4081 tggaacactc aagccaatca acatgagcgg tgacagccgc ccatgctttc ctggcgacct
     4141 acgagggatg cgcctgcttt gcctgtgcat acttcttttc ctctcctttg agtaggacta
     4201 tagcatgaac tgttgtgcgg acgtatatga tgtcctgata tccttccaga atcgccgcga
     4261 aagattttct cgcgctattt ctgaaatgct ttgatacaat ttgattacca acggtatgtc
     4321 aagatcatac atgatagaga aaaggctcgt tcactgttct ggtttttgtt gatataaaag
     4381 taccgtagag gcattgagaa atctcaacca cgtattctgc tgttatggta atttgttccc
     4441 atattgattg agaggtgtaa aaaccggagt gcttattcga ggaggctgca agtatactac
     4501 atacgtagac atgtgtgggc tattctgatg ctcttgcagg tacctccaac cgatcggtca
     4561 ggcggaggta cctacaggag gagcgacgat ctttcgctca ccccgtcgtc cagtcagata
     4621 tatcttcatt ccagcataga gaccacagac gccattgcct gttcttgaca gaaaatagtc
     4681 gcctttgggg tcatactcaa tgtaacccgg tatccgaaat ccgcccagca ggatatccac
     4741 agcctctccc cgcctcaact cacgcccgcc gggcgaaccc agggcaaaat gcctgttatc
     4801 ttgtagagaa ggataaatcg tccatgtatt catgtagatt gcctcgcttt ctactgagta
     4861 cttgaaagag atccctatcc ggtggtaatt gtgccatcgg cgtttacgcg aatggt
//
LOCUS       DASZAK010000133         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_500177, whole genome shotgun sequence.
ACCESSION   DASZAK010000133 DASZAK010000000
VERSION     DASZAK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500177"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="VGT44_02280"
     CDS             <1..1525
                     /locus_tag="VGT44_02280"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEV2579654.1"
                     /translation="GTREAVVCGQTRYTWEDFDQRTDALARGLASFGVARGDRVALLM
                     LNCHRYLELYYACARMGAVIVPLNIRLARPEVIFILNDSETKALVVDKTFAGYIVGRD
                     TVPSLEVVVYSGDETPADMIHYEDVVSKGTHMLESVDQDMNEDDISGLYYTGGTTGRA
                     KGVMLSHKNIMANAINVIMAAGYNQRDTYLHAAPMFHLADVGSVFAISMVGARHAIVP
                     AFNPAQVMQVIQDEQVTTVLLIPTMVNMVLNHPDVDNYNMASLRRLSYGGSPMPVEVL
                     KRGLRKWGQVFGQGYGLTEAAPLLTGLDTWEHVADGTPEQMRRLNSCGKEALGIEVRV
                     VNAEGEDVLPGEIGEIIARGDNIMLGYWRMPEATAAAIVDGWLHTGDMATIDDENYIY
                     IVDRAKDMIISGGENVYSVEVENALYTHPAVLEAAVIGIPSDTWGEAVHAVVVCKPGM
                     SVTADELIAHARTQVAAFKAPRSVELMTEALPKSGAGKILKRDLREPYWSGKSKNVN"
     gene            1541..>1911
                     /locus_tag="VGT44_02285"
     CDS             1541..>1911
                     /locus_tag="VGT44_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579655.1"
                     /translation="MSTTASPMSLDPTPWFRMMRESSPVFRNPRTQGWMVFRYNDVQR
                     VLSEYSTFSSQFTDSGEALEASMISSDPPRHRQLRSLVTLAFTPRTIAQLESRIAEIV
                     HEQLDKVTAQGRMDIVDDLSNP"
BASE COUNT          396 a          578 c          577 g          360 t
ORIGIN      
        1 cggaacgcga gaggccgtgg tttgcggaca aacgcgctac acctgggaag acttcgacca
       61 gcgcacggac gccctggcgc gtgggctggc ctcgttcggc gtagcgcgcg gggaccgcgt
      121 ggccttgctg atgctcaact gtcaccgcta tctcgaattg tactatgcct gcgcgcgcat
      181 gggcgcggtg atcgtgcccc tcaatatccg cctggcgcgc ccggaagtga tcttcattct
      241 gaacgatagc gagacaaagg cgctggtcgt cgataaaacc ttcgcggggt acatcgttgg
      301 gcgcgatacc gttcccagcc tggaggtcgt cgtctacagc ggcgacgaga caccggctga
      361 catgattcac tacgaagatg tcgtcagcaa aggcacccac atgctggaga gcgttgacca
      421 ggacatgaac gaagacgaca tctccggcct ctactacacg ggtggcacga cgggacgcgc
      481 gaagggcgtg atgctctcgc ataaaaacat catggcgaat gctatcaatg tcatcatggc
      541 cgccgggtat aaccagagag acacctatct gcacgccgcg cccatgtttc acctggccga
      601 tgtcggctcg gtcttcgcca tctcgatggt cggcgcgcgc cacgccattg tgccagcctt
      661 caacccggcg caggtcatgc aggttatcca ggatgaacag gtaacaactg tcttgcttat
      721 cccaacgatg gtgaatatgg tcctcaatca tcccgacgtg gacaactaca atatggcgag
      781 cctgcgccgc ctgagctatg gcgggtctcc catgccggtc gaagtcttga aacgcgggct
      841 gcgcaagtgg gggcaggtct tcgggcaggg ctatggactg actgaggccg cgccgctgct
      901 caccggcctc gatacctggg aacacgtagc ggatggtacg cccgaacaaa tgcggcggct
      961 gaactcgtgc ggcaaagagg cgctgggcat cgaggtgcgt gtcgtgaacg ccgaagggga
     1021 agatgtgctg ccgggcgaga tcggtgagat catcgcgcgc ggagataata tcatgctggg
     1081 ctactggcgt atgccagaag ccaccgccgc cgcgattgtg gatggctggc tgcatacagg
     1141 cgacatggcc accattgacg atgagaacta tatttatatt gtagaccgcg ccaaagatat
     1201 gattatctcc ggcggcgaaa atgtctactc ggtcgaagtc gagaacgccc tctacacgca
     1261 ccccgccgtg ctggaggccg ccgtgatcgg cattcccagc gacacctggg gcgaggcggt
     1321 tcacgctgtc gtcgtctgca agcccggcat gagcgtcacc gccgacgagc tgatcgccca
     1381 cgcccgcacg caggtggccg cgttcaaagc gccgcgcagt gttgaactca tgaccgaggc
     1441 cctgcccaaa agcggcgcgg gcaagattct caagcgcgac ctgcgcgagc catattggtc
     1501 tggtaaaagt aaaaacgtca actaatgaaa cgaggtttcc ttgtccacga cagcatctcc
     1561 gatgagcctt gatccgacgc cctggtttcg catgatgcgc gagtcctcgc ccgtctttcg
     1621 caacccgcgc acccagggat ggatggtctt ccgctataac gacgtgcagc gcgtcctttc
     1681 ggaatacagc accttctcct cgcaattcac cgactccggt gaggcgctgg aagcgagtat
     1741 gattagcagc gacccgccgc gccatcgcca actgcgttcg ctcgtgacgc tggccttcac
     1801 gccgcgcacc attgcgcagt tggagtcgcg catcgctgag attgtgcatg agcaactcga
     1861 caaagtgacg gcccagggtc gcatggatat cgttgacgat ctctccaacc c
//
LOCUS       DASZAK010000134         7977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_677737, whole genome shotgun sequence.
ACCESSION   DASZAK010000134 DASZAK010000000
VERSION     DASZAK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7977
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677737"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            216..602
                     /locus_tag="VGT44_02290"
     CDS             216..602
                     /locus_tag="VGT44_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579656.1"
                     /translation="MRIRPIFWCILALSCLGVLVFAATIPIVAPAVMQARLDSPAPDS
                     ASFTTIELRLSDSQGLPIEQARVTPSARMTNMDMTTSTILVQSRGQGTYIVQLELFMS
                     GPWEIDISARADGFDAAQQTLLVQVL"
     gene            619..1728
                     /locus_tag="VGT44_02295"
     CDS             619..1728
                     /locus_tag="VGT44_02295"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2579657.1"
                     /translation="MPNILLSHPTSSKIRARWRLSLFEKAILINTLLLIGEALAGLWI
                     TSHSLETHHYLIDTSFIVAATLLSVLINVLLLRASFRPLFSLLRTIRAVGAGGLNLRA
                     SDVPIHSEVGELALAFNTMLDQIESSRRQQAMLILQAQEEERRRVARELHDESSQNLT
                     ALLVHSEILSQTIQAIPDRAVTQDMRVRLDTELRQLTQLTQHTLESVRALALQLRPGI
                     LDDLGLNAALRWLAGDFRQRLHLPVELSIDGLEESLQASPYTALYATTLFRIAQECLT
                     NAARYAQAQHVFISFQQGNQHISFFVRDDGNGFDPASQTPGLGILGMRERAALLGGTL
                     TITSSHGKGTAIEARLPLLPNAPGVNEEPAYVVHA"
     gene            1712..2422
                     /locus_tag="VGT44_02300"
     CDS             1712..2422
                     /locus_tag="VGT44_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2579658.1"
                     /translation="MLSTPETGANARPIRVMLADDHDILRQGLKMLLSLQTDIEIVGE
                     ARTGREAILLAGELRPDVAVLDITMPDIDGLEACRRIRQEAPDTQVLILTMHESEEYF
                     LQALRLGAAGYLVKKAAPTELHMAIQAVARGDAFLYPGLAKALVRTFVTQAAESPSPQ
                     KELSPGPQLRLLSPREIEVLKLVAEGNTNQEIADRLVISIKTVQAHRASVMEKLGLHD
                     ITHLVRFAIHTGLIPPDP"
     gene            2580..4577
                     /locus_tag="VGT44_02305"
     CDS             2580..4577
                     /locus_tag="VGT44_02305"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEV2579659.1"
                     /translation="MIPRVHNRRMRRLLLIAVPMALLGVLLSPAIASAHAILLRSNPT
                     SDAQLPNAPQSVQMWFTEDLNPAFSSAVVINAENKRVDNQDSHVSSTDPTEMELTLKP
                     NLPGDVYIVVYRTDSAIDGHVLRGSFIFYVANADGSFPSLSPNANPGSNIFGGGNVTG
                     LYTGQIDGPTFFNLLMVTLVELGAVFWVGAQLWLNFVLQPSTERYAEEKDTNERVEQR
                     FERRFSLPTLLVLLLANIGVLVGQAINLTGGDWGQALSPTLLAQLATSGRFGTFWLMR
                     EIVILLAIVAALYTLFVRRRPRPINLLLPLLNLLLGTALFIAITMSSHAAAVSDNLVS
                     YAIVVDWLHLLASALWVGGMFYIAAIYLPILKKQDTASTARSLIHLLPAYSILAIAGV
                     IIMSITGPFSATFHLNSWSQFLDTAYGRALLVKIGLVGTLLITSAIHVGILRPRVKKE
                     YKKYSYAAARLAAFETTAIAEQQTVPVAIPAQPGLPAHANAPRLLTQQVRLREQRLAS
                     KTRRLTSVLRWEPLLGVAVIVCVGLMNAFGGTLTPFAAQQQSNGKPQIFSQTVHTQDG
                     KFNATLVINPNRFGTNVFTVSVVNNATGKPVTNNIGVVIYTTMLDMDMGTQSVNLLPD
                     GKGHFSARGDLSMPGNWQIRIQIRTLDNTLHEASVNIDTPF"
     gene            4623..5687
                     /locus_tag="VGT44_02310"
     CDS             4623..5687
                     /locus_tag="VGT44_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579660.1"
                     /translation="MKSIKAYALVIALLLILAGCSTDNTGPQTTPQATIAPVNGFGIA
                     ANHVHSLLALSSQTLLLATHYGIYRSLDGGATWQQETSGPGQLMAGLMEYAMTVSPLD
                     PQRLFVLTQPAVPGYQGTPGLYTSADGGKTWKLSIASSSISSNYIFTEAAGNDSPDEV
                     YIYLTSLGNLGLKRSLDDGQHFSGAGALPFDLIYGMAIVPGAPGYLFVYSSEGIASTS
                     DGGIHWHVIAGINGGIEDMVIAGANLPIYASGDQGIYASTDGGKTFKLVYTQASYSSL
                     AVSAQQPQTIYGKTGSNIYRSTDGGKTWTTLPHIGGNLAVLAVVPGHPSEVYLSLSYP
                     TAMYQLNASGTAWTSLTPPA"
     gene            5712..6854
                     /locus_tag="VGT44_02315"
     CDS             5712..6854
                     /locus_tag="VGT44_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579661.1"
                     /translation="MKLYNRLARLLISVWGIILLVSLFAIVTPAFADGGAPNLAYISG
                     ASSGIGVLDIQQQKVTTTFNLTGNPQTIYLSLDGRFLYVTQPAMNRVTMLAAKTGDTL
                     CTVNVPGQPSLLAYDPGANMLYAAGNGSSSVTEFDPNTCAIKRTITTNGPVYGLAVAN
                     IGTSSAGNQLWVAASTLEVFDNTGRIASIAIPGEPRYVNIPAGTMVYVTTHDGHVYAV
                     SLGTRSLLLPALLSGGQYGPMDYDAYTGQVYVPDMLNKQVDVLAPVVSNTPPLPHEPD
                     HIIHLGATPISVAITSDGQFGFVALQGGNVAILDVPGKAIYNTVFVGGNPRFIITGLY
                     PPSLLTTPQQVSTWGTVINIAAYVFVFLLLIVPIVFIGRRMRTKKG"
     gene            complement(6851..7252)
                     /locus_tag="VGT44_02320"
     CDS             complement(6851..7252)
                     /locus_tag="VGT44_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579662.1"
                     /translation="MATPLVIVITLWITGFLSVLNMACAIYFRKRRAVEIVSYVAAGL
                     VELGIFVFALLLHFGVLKHIPYHLPPGLPINRAEIGAALAIGVGLLPVTYWHRSSSTR
                     IRAMMAKEAEEMRNRDAAVRVRSAAPGEWMN"
     gene            complement(7267..>7977)
                     /locus_tag="VGT44_02325"
     CDS             complement(7267..>7977)
                     /locus_tag="VGT44_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579663.1"
                     /translation="EIGTHPSGQVETGQTTVSITPNVGATATARSEATLEATTILYDP
                     LSMNIHNWPESATGAILYQFKNGAYHISNNDPSRIAPAILQNENLDQPYTYTLTMDQI
                     RGDDTSINNEYGMIVRFSARQLNGKQAISFYSFEILNKQGGQYQFWKYDNSSGTGNPW
                     TQLAQHPLGNEFHQGLGAQNMNTVTIQVNGKNFTLIVNGKKIWNVSDGALTGGSVGML
                     VNLKGAEVAFSDLRLTDD"
BASE COUNT         1723 a         2582 c         2033 g         1639 t
ORIGIN      
        1 gaacgccctt ctggctggct gccatacttg cccgagtaag tctggtaatc gggctgggcg
       61 caggactact cctcggacgc aggagttcga ttcatccgtc aagcgcggct gctgtttccg
      121 gccttagcgc gatgcgccgg agcatgaagc aagagcagga tgaatagcga tcacgccgac
      181 agtcacgaga catgccggtg caaggagaac ataccatgcg cattcgacca atattctggt
      241 gcattcttgc tctttcttgc ctcggtgtgc tggtattcgc cgccaccatt cccatcgtcg
      301 cgcccgccgt catgcaggcg cgcctcgatt cacccgcgcc ggattcggcc agcttcacca
      361 ccatcgaatt gcgcctgagc gactcccagg gcttacccat cgagcaagcc cgcgtgacgc
      421 ccagcgcgcg tatgaccaac atggacatga ccaccagcac gattctggta cagtcgcgtg
      481 ggcagggtac gtatattgta caattggaac tgtttatgag cggtccctgg gaaatcgaca
      541 tctccgcgcg cgccgacggg tttgatgctg ctcagcagac cttacttgtg caggtgttgt
      601 gagaaagcga gtcgttccat gccaaacata ctactatccc accccacttc ctcgaaaatc
      661 agagcacgct ggcggctttc tctctttgag aaagccatac tcatcaacac gctcctgctc
      721 atcggagaag ccctggcggg tctctggatc accagccaca gcctggaaac gcatcattat
      781 ctcatcgata ccagctttat cgtggccgcc accttgttga gcgtcctcat caatgtcttg
      841 ctcctgcgcg ccagctttcg ccctctgttc agcctgctgc gcacgatacg cgcggtaggc
      901 gcgggaggtc tcaacctgcg cgcaagtgat gtccccatac attcggaggt cggcgagctg
      961 gctctggcct tcaatactat gctcgaccag atcgaatcgt cccggcgaca gcaagccatg
     1021 ctcattctcc aggcacagga agaagaacgc cgccgcgtcg cccgcgaact gcacgacgag
     1081 agcagccaga acctgaccgc gctgctcgtc cattccgaga tcctcagcca gaccatacag
     1141 gccattcccg atcgagctgt cacgcaagac atgcgcgtcc gcctcgatac agaactgcgc
     1201 cagttgacac agttaacgca gcacacctta gagtccgtgc gcgcgcttgc cctgcagctg
     1261 cgccccggca ttctggatga cctcggcttg aatgccgctt tgcgctggct cgccggggat
     1321 ttccgtcaac gtctccacct gccagttgag ttatccatcg atggtctgga agaatcattg
     1381 caggcaagtc cttacaccgc gctttacgcc accaccctgt tccgcatcgc ccaggaatgc
     1441 ctgaccaacg cggcgcgcta cgcccaggcg cagcatgtct tcatcagctt tcagcagggc
     1501 aatcaacata tttccttctt cgtgcgcgac gacggtaatg gcttcgaccc tgccagccag
     1561 actcccggct taggcattct cggtatgcgc gaacgcgccg cgttactcgg cggaacgctg
     1621 accatcacct cttcacacgg caagggaacc gccatcgaag cccgtctgcc gctcttgccc
     1681 aacgcccctg gcgtcaacga ggaacctgcc tatgttgtcc acgcctgaaa ctggcgcgaa
     1741 tgcgcgcccg atccgtgtca tgctcgccga cgaccacgac atcttgcgcc aggggctgaa
     1801 aatgctgctc agcttgcaga cggacatcga gatcgtgggt gaagcccgca ccggtcgcga
     1861 agcaatccta ctggctggcg aactgcgccc cgacgtggca gtcctggaca tcaccatgcc
     1921 cgatatcgac ggcctggagg cttgccgacg cattcgccag gaagctccgg acacacaggt
     1981 gctgatattg accatgcacg aaagcgaaga atatttcctc caggctctgc gcctgggcgc
     2041 tgccggctac ctggtgaaaa aagccgcgcc taccgagctg catatggcta tccaggcggt
     2101 cgccagggga gatgcattcc tctaccctgg cctcgccaaa gccctcgttc gcaccttcgt
     2161 gacccaggca gccgaatcgc cctcgccaca aaaagagctt tcacctggcc cgcagctgcg
     2221 cctgctcagc ccacgcgaaa tcgaagtact caagctggta gcggagggga acacaaacca
     2281 ggagatcgca gatcggctgg tcatcagcat caaaaccgtc caggcgcacc gcgcaagtgt
     2341 catggagaaa cttggcttgc acgacattac acacctggtt cgcttcgcta ttcataccgg
     2401 tctgatacca cccgacccct gaccacgttg taaggatagc cgtaataaag gagaataaag
     2461 tcatacaatc aacgtaataa caggttgatt gtgtagcgca aagcgtaatt ataataggaa
     2521 gaagtaatct tcgtaatcat caaacatcgt tgttaaacaa ctaagactgg accctgataa
     2581 tgatacctcg cgtacacaac cggcgtatgc gccgacttct actgatcgcc gtgccaatgg
     2641 cgttgctcgg cgtcttatta tcccccgcca tcgccagcgc gcacgctatt ttactgcgct
     2701 ccaacccaac gagcgatgcc cagctgccca atgcgccgca gagcgtgcag atgtggttca
     2761 ccgaggacct gaacccggcc ttcagttcgg cggtcgtcat taacgcagag aataagcgcg
     2821 ttgacaacca ggactcgcat gtctcatcca ccgacccgac cgagatggaa ctgacactta
     2881 agcccaacct gccgggagat gtctacatcg tcgtctatcg caccgactca gccatcgatg
     2941 gccacgttct gcgcggctcg ttcattttct acgtcgccaa tgccgacggc tcgtttccct
     3001 cactcagccc gaacgccaat cccggctcga atatctttgg cggcggcaac gtcaccggac
     3061 tctacaccgg ccagatcgac ggcccaacat tctttaacct gttgatggtc acactcgtcg
     3121 aactgggcgc tgtcttctgg gtcggcgcgc aactctggct caacttcgtc ctgcaaccct
     3181 cgaccgagag gtacgcagaa gaaaaagaca ccaacgaacg ggtagagcag cgcttcgagc
     3241 ggcgtttctc gctgccaacc ctgctcgtgc tgctcctcgc caacatcggc gtgctggtcg
     3301 gacaggccat caacctgacc ggcggcgact ggggccaggc gctctcacct accttgctcg
     3361 cacagctggc taccagcggg cgcttcggca cgttctggtt gatgcgcgag atcgtcatcc
     3421 tgctggcaat cgttgccgcc ctctacacgc tgtttgttcg ccggcgtccc aggccgatca
     3481 acctgctgct gccgctcctc aatttgctgc tcggcactgc cctcttcatt gccataacca
     3541 tgtccagcca tgctgcggcg gtgagcgaca acctggtctc ctacgcaatc gtggtggact
     3601 ggctgcatct cctggcgtcg gcgctgtggg tcggcggcat gttctatatc gccgccatct
     3661 acctgcccat cctgaagaag caggacaccg cttctaccgc ccgctccctg atacacctgc
     3721 tccccgctta ttctatcctg gccatcgcgg gtgtcatcat catgtccatc accgggccat
     3781 tcagcgccac attccacctg aactcgtgga gccagttcct cgacaccgcc tatggccgcg
     3841 ccctgctcgt caaaatcggg ctggtcggta ccctgctcat caccagcgcc atccacgtcg
     3901 gcattttgcg gccccgcgtc aagaaagaat acaaaaaata tagctacgcg gcagcgcgac
     3961 tcgccgcctt tgagacaact gctatcgcag agcagcagac cgtcccggta gctatcccgg
     4021 cacaacccgg gctaccggct catgcaaacg ctcccaggct gctcacacag caggtgcgcc
     4081 tgcgcgaaca acgcctggcc agcaagaccc gcaggctgac ctccgtgttg cgctgggagc
     4141 cgctgctagg cgtggctgtg atcgtctgtg tcggcttgat gaatgccttc ggcggcacgc
     4201 ttaccccctt cgccgcgcag cagcagtcca atgggaagcc gcaaatcttc agtcagaccg
     4261 tgcataccca ggacggcaaa ttcaacgcaa cgctcgtcat caatccgaac cgcttcggca
     4321 cgaatgtctt caccgtcagc gtagtgaata acgccaccgg caaacccgtc acgaataaca
     4381 tcggcgtcgt catctacacc accatgctcg acatggacat gggtacccag agcgtcaacc
     4441 tgctgccgga cggcaaaggc cacttcagcg cgcgcggcga tctctccatg cccggtaact
     4501 ggcagatacg cattcaaatt cgcacactcg ataacacctt gcacgaagca agcgtaaata
     4561 tagacacgcc attttaatag ctcgccccta cgggtctctt gaaaaaatgt gaggtctctg
     4621 caatgaaatc aataaaagca tatgcgcttg tgatcgcctt gctgctcatt ctggctggat
     4681 gtagcacgga taacaccggc ccgcaaacca cgccgcaggc gaccatcgcg ccggtgaacg
     4741 gcttcggcat cgccgccaac cacgtccatt cgctgctggc cctgtcatct caaacgctgc
     4801 tgctggcaac gcactacggc atttaccgct cacttgatgg cggggcaacc tggcaacagg
     4861 agacgagtgg ccctggccaa ctgatggcgg gtctgatgga gtatgccatg acggtcagcc
     4921 cgcttgaccc acagcgcctt ttcgttctca cccagccggc cgtccccggc taccagggaa
     4981 cgccaggact ctataccagc gccgatggag ggaaaacctg gaaactctcg atcgcttcct
     5041 cctcgattag ctcaaattat atcttcaccg aggctgccgg caacgattcg cccgatgaag
     5101 tttacatcta tctgacctcg ctcggcaacc tggggttgaa acgcagcctg gacgacggac
     5161 aacatttctc cggcgcgggc gcgctgccct tcgacctcat ttacggcatg gctatcgtac
     5221 ccggcgctcc cggatacctc ttcgtctaca gcagcgaagg catcgccagt accagcgacg
     5281 gcggcattca ctggcatgtc atcgcgggca tcaacggcgg tattgaggat atggtcatag
     5341 ctggagcgaa cttgccaatc tacgcctcag gcgaccaggg aatctatgcc tcaaccgatg
     5401 gcggcaaaac ctttaagctg gtctacaccc aggcatcgta ttcatcgctg gcagtttccg
     5461 cccagcagcc acagaccatc tatggaaaaa cggggagtaa catttatcgc agcacagacg
     5521 gcggcaaaac ctggacgacg ctgccccaca tcggcggcaa tctggcggta ctggccgtcg
     5581 tgccaggcca cccctccgag gtctacctct cgctctccta cccgaccgcc atgtaccagc
     5641 tcaatgcatc gggaacggcg tggacatcgc tcacgccccc ggcatgacac tatcataaag
     5701 gacgtattat catgaagtta tataatcgcc ttgcgcggct actaatcagc gtctggggta
     5761 taattttgct cgtatcactt ttcgcaatcg tcactccggc cttcgcggat ggcggcgcgc
     5821 ccaacctggc ctatatctcc ggggcttcca gtgggatcgg cgtcctggat atccagcagc
     5881 aaaaggtgac gacgaccttc aatctgacag gcaaccctca aaccatctac ctgagtctgg
     5941 atggacgttt cctgtatgtc acacagcccg ctatgaatcg cgttaccatg ctggcggcaa
     6001 agaccggcga caccctctgt acagtcaacg tgccgggaca accatctctg ctggcttatg
     6061 atccaggagc gaatatgctc tacgcggcgg gcaacggcag ttcaagcgtc accgagtttg
     6121 acccgaatac ctgtgccatc aagcgcacca tcacaaccaa cgggccggtg tacgggctgg
     6181 cagtcgccaa tatcggcaca tccagcgccg gcaaccagct ctgggtggcc gcttctaccc
     6241 tggaagtctt cgacaatacc gggcgcatcg cgtccattgc catccctggc gaaccacgct
     6301 acgtcaacat tcctgccggg acgatggttt acgtcacaac gcacgatggt cacgtctacg
     6361 ccgtcagcct gggcacgcgc tcgctgctgc tgccggccct actgagcggc gggcaatacg
     6421 ggccaatgga ctacgacgcc tataccggcc aggtctatgt gccagatatg ctcaataagc
     6481 aagtcgacgt gctggcgccg gtcgtctcca acacgcctcc tctgccccac gaacccgacc
     6541 acatcattca cctgggagcc acaccaatct ccgttgccat caccagcgac ggccagtttg
     6601 gctttgtagc cctccagggc ggcaacgtcg ccatcctgga cgtacccggc aaggccatct
     6661 acaataccgt ctttgtcgga ggcaatcccc gcttcatcat caccggcctc tatccgccca
     6721 gcctgttgac gaccccgcag caagtctcaa cctggggaac ggtcatcaac atcgcggcgt
     6781 atgtcttcgt cttcctattg ctgatcgtgc cgattgtctt catcggcagg aggatgagga
     6841 cgaagaaagg ctaattcatc cactcgccag gagccgcgct gcgcacgcgc acggctgcat
     6901 cgcggttgcg catctcctcg gcctccttcg ccatcatcgc gcggatgcgt gtggaggagg
     6961 agcgatgcca gtaggtcact ggcagcagcc cgacgccgat agccagcgcc gcgccgatct
     7021 cagcgcgatt gatcggcaag ccagggggaa gatggtaggg aatatgtttg agaacaccga
     7081 agtgcagcag cagcgcaaac acaaaaatgc ccagttcaac caggccagcc gcgacatacg
     7141 aaacaatctc aaccgcccgt cttttacgga aatagatggc acacgccata ttcaaaacac
     7201 tcaggaatcc ggtaatccac aacgtaatca caatcaccaa cggtgtcgcc atctgttgcc
     7261 tccatactaa tcatcggtca agcgcaaatc cgagaacgcg acctcagcgc ctttcaggtt
     7321 caccagcatc cccacactgc cgcccgtcaa cgctccatcc gagacgttcc aaatcttctt
     7381 tccattcaca atcaacgtaa aattcttgcc gtttacctga atggtgacgg tattcatatt
     7441 ctgcgctccc agtccttgat gaaattcatt accgagagga tgctgcgcca gttgcgtcca
     7501 gggattgccc gtgccagagc tattatcata cttccagaac tggtattgac cgccctgttt
     7561 attcaggatt tcaaaggaat agaagctgat agcctgtttc ccattcaatt gccgcgccga
     7621 aaaacgcacg atcattccat attcgttatt gatcgaggta tcatctcctc gtatctgatc
     7681 cattgtcagc gtataggtat aaggctgatc caggttctcg ttttgcaaaa tagctggagc
     7741 aatgcgcgat gggtcattat tgctaatatg atatgcccca tttttgaact ggtagagaat
     7801 agcgccggtc gcgctttccg gccagttgtg gatgttcatc gacaatggat catagagaat
     7861 agtagtagct tcaagcgtcg cctcagacct ggcggtagcc gtcgccccca cattgggcgt
     7921 aatgctgacg gtggtctgtc ccgtttctac ctgtcctgaa ggatgtgttc caatctc
//
LOCUS       DASZAK010000135        14095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_180183, whole genome shotgun sequence.
ACCESSION   DASZAK010000135 DASZAK010000000
VERSION     DASZAK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 14095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14095
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180183"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..1404
                     /gene="dnaN"
                     /locus_tag="VGT44_02330"
     CDS             265..1404
                     /gene="dnaN"
                     /locus_tag="VGT44_02330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV2579664.1"
                     /translation="MKVTCKQQDLSRGLSAVSHAVSSRSTLPILLNILMATDHGRLKL
                     SATNLEIGINCWVDAQIHEEGATTVPAKLLTDLVSRLPQMPVDLAVSEDSHTLNVKSQ
                     GSNANIKGMDASEFPLIPSAEGGEPPVLLDATLLKEMIAEVAFAAADDDSRPVLTGVL
                     VQVSDEKLTFAAADAFRLAVRVAPLPGDGHPRGDILIPARTLTELARILPSEGTVEMI
                     VTPNRSQVLFHTTNLDLVSRLIEGTFPNFRQIIPQDHSTRAVVETKDFAEKVKCAELF
                     ARDSSNITRVKINPGANDGLEPGAVTIEATAEDLGDNTSNIAAAVDGAEMQIIFNVKY
                     LADVLSVIGTPEVALEATSPTKPGVIRPVGASDYTYVIMPMHINR"
     gene            1422..2606
                     /gene="recF"
                     /locus_tag="VGT44_02335"
     CDS             1422..2606
                     /gene="recF"
                     /locus_tag="VGT44_02335"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV2579665.1"
                     /translation="MHLSHLVLNDFRNFKHLDLPLGRGLSLFYGENAQGKTNLLEAVS
                     MLATSNSFHATSDREVVNWDAPDRIARIIADIERGEDEVKVEIVIIDASPIDANGESA
                     YASQLQTPDRQRKRIKINGGIKKATDLIGQVTVVLFAPTDLRLVDGSPEDRRRFLDRA
                     LCQVQPRYCAALARYRKVITQRSALLKRIRENQEDPRMLEFLDEKLIELASQIIYERQ
                     RMVRALNTHVDGLQTLISGGREGLQIVYRPAFEVDPAWNTLDAQEHYRRQLHAARRKE
                     IQQGVCLLGPHRDDLDFMANDVNLLTYGSRGQQRTAALSAKLAELEFMRATTGEEPIL
                     LLDDVFSELDAARREYLLRLALDHEQALVTATDMTGIPEEMLAQIHRFSVVAGEITHT
                     HF"
     gene            complement(2591..4294)
                     /locus_tag="VGT44_02340"
     CDS             complement(2591..4294)
                     /locus_tag="VGT44_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEV2579666.1"
                     /translation="MRPVPIQPMQMPPEDRGKSGHRGRGTALSPAQWLRQHVLAQEEE
                     TIASLGELLLPTLLGAMEACWVCAALIGLASAHLFGFSTPLLPLWSPFVYIIGFHWTL
                     VLRERRVASIQQATSEQEAGSKNSKDGQASVVRQDMPLFFVMLVVLALFLIWLQIYAP
                     TRPLYDLTWLATLGSDTLFLDLHFYQALMMVLFSCYLCWRGLRLLSHTVEPSQVIRGL
                     LLGLGVIIAAILLLASVESSGATSPDAIFLFMLIPVFLFLSLVAHALARIVFVRKSHF
                     SGLQGSVVAQERAVITVVASLGIILLLVTLLVGLFADPTFFSSALSVLMPVGIAIGNA
                     YNWLVGIFADILVFLATPIFWLFTFLGSLLPHGKPGTQNIPKQNQKQKLPTHPITNIN
                     AAWLPFLRVGLLLVLLLGLVCLVWWAVQHRRRKVLRLRRKDADIHESLWSWLLLWNQV
                     KNILRALFGRFSRYKPRAETQVAGAQGTHPSDPAVRTIREIYRAVLKKAAGRGYQRKK
                     YETPLELRQRLDEGVPLVEPQLESITEAYSMVRYGDSLPNVDDVIAVGEQWRELDQKW
                     V"
     gene            complement(4311..4814)
                     /locus_tag="VGT44_02345"
     CDS             complement(4311..4814)
                     /locus_tag="VGT44_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579667.1"
                     /translation="MDEIQEQQEHTNGQAAGKVDYRPLTRIERRNSWLEEYGKQDFAL
                     QMWTRLVEQQDLEIEMMLQMHGLLVFGVMVSTLRYAQFYIDLNEELHREHEPETADAL
                     REYYYALVPPRDQPEIGPEGLPTIYRIAHMRDVTIMSAGHKIKVPYWRGKISDVDAFV
                     VGATAAE"
     gene            complement(4900..7329)
                     /locus_tag="VGT44_02350"
     CDS             complement(4900..7329)
                     /locus_tag="VGT44_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV2579668.1"
                     /translation="MDEQDIILSDETLSRAASATQISPVPRPVSGPLAAIAPEMARRR
                     ARVNHVMFIKRRHTRQERSEQTGPRLMILLLVLSAILITLLSGAAGGAFAYYKAQLPL
                     LDNIASHSLFQTTHIYDRNGNLLFDLNDPRYGRRTYVNYTEISQNLVNATVAAEDRTF
                     WSNSGIDVQGILRAAVTNLQNQTVVEGGSTITQQLIKQQLFANDPRTLQVKGEEAILA
                     YGLTQQYPKWKIMEMYLNTVYYGDLNYGIEAAAQDYFNIQPKCTQKTCRSAASQLDLA
                     QASLLAGLPQLPTYYNPIENKAVALDRQKYVLQSMIDLGMITDTQAKQAEAETAKFNF
                     QSYSSRQGIKAPHFVQYVIDDVLAPMIGAQNLYDGGYNIYTTLDLHLEQYVEKSVYNH
                     LYTQQCDNYLGCYGPLDTTNNVNNAAVVVENPFNGEILAMDGSASYNSSRPQVSGHFN
                     AATALRQPGSSFKPIVYATAFEMGWYPAMILGNHKTTYPTLISSNPVQYYSPNNYGNT
                     YLSGFPMTIRNAIANSINIPAVDAIEFAGIQNVVNTAGRLGLTEIANKPLSQLGPSMA
                     IGASEVSLLHLTGAYATFADQGVRVPQTSVLEVTDSQGRVLYKYDQAHPAGVQALRPD
                     VAFLMSSILSDKTSRYHEFFPGNPLELDRPAAAKTGTTDSFRDNWTMGYTPYLTVGVW
                     AGNSDNSLMQNVIGITGAGPIWHDIMEYASQYYHYSPDDFSRPSDVHAGTVSALTGLL
                     PHAGEPTVTDWFIDGTMPTVQGPYTPPIQNCGNGGNGGNGGNNNGCPQPPQPIGPPIC
                     FPFNCGPPQGQ"
     gene            7606..8232
                     /locus_tag="VGT44_02355"
     CDS             7606..8232
                     /locus_tag="VGT44_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579669.1"
                     /translation="MSQQEFVPRSQQNPPPENEEEIYEPQHPYSWSGKSKSEAEPRDE
                     PPSSYEYEAGYQAQDAYDQNAYEHEAPFDPANSYTPLAEQAPQVQQQQQYQNPYDANT
                     YGQNSYGQDYNAYNNYKSAQSNSYQNVPPYARPQRQSGGSPWRFSSILLILVLITIFS
                     SVGRDFFFYGFHGFDMIIGFFPILILLLIFNGIRRGGRRRFRRGPWGW"
     gene            8239..9147
                     /gene="rsmA"
                     /locus_tag="VGT44_02360"
     CDS             8239..9147
                     /gene="rsmA"
                     /locus_tag="VGT44_02360"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV2579670.1"
                     /translation="MSEIEQVDRRARFSRGIRDDVDLTDIHELRSLLYAHHMRPNKSF
                     GQNFLVDRAILQQVVDAAQLEPGDQVLEVGAGTGVLTRELAKQARRVVAVEIERDMIE
                     LLRETTGHYPHVELIERSLLALDPCEVFDQEPYKLVANLPYYITAPTFRHFLESRNPP
                     RLLVVMVQREVAQRIVAGPGDLSLLGVSIQFYGKPELVAHVPARAFFPSPLVDSAILR
                     VEVHDQPPLTRKERDSFFRVVQAGFAEKRKQLHNALTHHLHRKNEDIRACLVAANIDP
                     GRRAETLSIEEWLRLWNILQQPKTSL"
     gene            9233..11536
                     /locus_tag="VGT44_02365"
     CDS             9233..11536
                     /locus_tag="VGT44_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2579671.1"
                     /translation="MEEVEDLEEQQQPATPPALTGDTDDNTVKTDIGSESSGSADTIA
                     PEESPTQTTLADEIAGQDAPTTQPVGVDATTEALFCDDCGAANPAGSRFCQHCGSLIP
                     FQRHTGTLPMQIMLAGHYQLLSRIGQGGMGAVYKAADTKLNNRSVAIKEMNRSGLSST
                     RLKEAEDAFEREAHLLADLVHPNLPRIHEHFTENERSYLVMDFIEGQTLEEYLEKTGG
                     GPVPVDVVVRWATELCDVLNYLHTRQPPIIFRDLKPANVMISETGHIFLIDFGIARLF
                     KPGKQHDTVVLGTPGYAAPEQYGKTQSSPRSDLYSLGALLHHLLTGIDPSEHPFFFSP
                     ASQVNPAVNPMLERLLKQMVEMDSGKRPTSAQEVLRTLESATTRPTSAMLANTSSTMS
                     ITDPLLEQAYRAYSQKRLDEALKLYSQALQKDSSSSLAWQGYGLTQGLRAQHQDALTS
                     FERALQLNPGLVTSWNGKGTALSRLRRHQEALVAFERAVELDPNNAAAWNGKGATLNA
                     LGRAALALDAFDMSISHDPQLIQAWNNKGLVLYELGRFKEAQKAFEEALDLDSHNAHA
                     LFGKGQALYGQRKLKQAFTLYEQALRVDDTFADAWNRRGNVLDEMGDNGAALQSYDRA
                     LQLNPRLVAAWNGKAGVLRKLKRLFDAFTAYNHALDIDPKFALAWNGKGNTLFDMKNY
                     AEALLSYERALQYNQRLATAWHNKSLVLYRLGRYDEALRAADEAIHMRPDDPDNWQRK
                     ADALKALNRRQEASFAERQVIYLKGRA"
     gene            complement(11537..11938)
                     /locus_tag="VGT44_02370"
     CDS             complement(11537..11938)
                     /locus_tag="VGT44_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579672.1"
                     /translation="MKMSRAKKIVVYLVIPPLLFAVAIVLVVFLYLRFHALVEVDVTT
                     SGFARSTVEITEGETIHIVNQSNVMQTICLGQDRTCDTSAVAPVVLKSPGIRLAPGAS
                     VDVVFELYGSYAVTSTSAPGVNLQITVDAGG"
     gene            complement(12041..13042)
                     /locus_tag="VGT44_02375"
     CDS             complement(12041..13042)
                     /locus_tag="VGT44_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2579673.1"
                     /translation="MRVVSLGSGSSGNALFIEAGPRGRTSILIDAGFPGRILVERLRQ
                     AGVSPARLSGILVTHEHTDHIIGLPFLVKRYKIPALADPRTLAAIEKTLAYSSLSAPF
                     PSLTYPSVRAGAGLGGEGTLASPAVLPDDANITADEPEMETGIDDAPDVIEHGSSAFP
                     VGSRRMIGDIEVRSFPVSHDAVAPCGYLLSAGGCRVCVVIDTGEVTDAMLEAINQADL
                     LVLESNHDRERLLRGPYPYHLKQRILSPSGHLSNDQAAGAVLRTWRSDGVRWLWLSHL
                     SRTNNTPRLALDSMRTRITEAKANPAQVHLSVLPPGLGCEWDSTQLWHQESLWEMPH"
     gene            complement(13046..13891)
                     /locus_tag="VGT44_02380"
     CDS             complement(13046..13891)
                     /locus_tag="VGT44_02380"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2579674.1"
                     /translation="MAMLIDFHTHIFPPDIVRRREYYCERDRWFGELYRKSRAVIASA
                     DDLVAEMDATGVDVAVTFSFGWTDPALIVETNDYVIDAMRRYPGRIYGMSVCLPTSGD
                     QAIHELERCAQAGMIGVGELMPHGQGYQLSNIPLLAPLMEVVRRYRLLVLAHCSEPVG
                     HLYPGKGNVSLPDIVAFLSAFPDIRFIAAHWGGGLPFYTLMPEIQAITTNVWYDTAAT
                     IYLYGARIFPAMVALVGADRILFASDYGLLRQKRVIDHIRQSGLDETSVRSILGENAR
                     QLLGL"
BASE COUNT         3123 a         4134 c         3940 g         2898 t
ORIGIN      
        1 tttctccatt ccaatcacgt gtgacagtcc aatagtcatc ggcgcagcga accaccccgt
       61 acagccgcca gaacgccgct tccgccttat agagcgcccc cgacttttcg cccattgaaa
      121 agaataggaa accatagggg ctaatcgcta acttagggcg gttttatact atatgcagga
      181 gcataacgtt tggtttacag cttcgtgaaa atcctgtata ctgagcggga tgttttgtgg
      241 gctatttacg gaggaagcta ttccatgaag gtaacttgta agcaacaaga tctgagccga
      301 gggctgtcag cggtaagcca tgccgtctca agccgcagta cactgcctat tttattgaat
      361 attttgatgg cgaccgatca tggtcgtctg aagttgtccg cgaccaacct ggaaatcggc
      421 atcaattgct gggtcgatgc gcagatacat gaagagggtg caaccaccgt gccggccaaa
      481 ctgctgaccg acctggtgag ccgcctgccc cagatgcccg ttgatcttgc ggtgagcgag
      541 gattcgcata cgctcaacgt caagagccag ggcagcaacg ccaatatcaa aggcatggac
      601 gcctccgaat ttccgctgat tcccagcgcc gagggcggcg agccgcctgt gctgctggat
      661 gccacactct tgaaagagat gatcgccgag gttgcctttg ccgccgccga cgatgactcg
      721 cgacccgtgc tgacgggcgt ccttgtgcag gtgagcgacg agaagttgac cttcgccgcc
      781 gccgatgcgt tccggctggc agtgcgcgtc gcgcccctgc ctggcgatgg acacccgcgc
      841 ggcgacatcc tgatcccggc gcgcactctg acagaactgg cgcgcattct gccatccgaa
      901 ggcaccgtcg agatgatcgt cacgccgaac cgcagccagg tgctgttcca taccaccaat
      961 cttgacctgg tttcgcgcct gatcgaaggc accttcccca atttccgcca gatcatccca
     1021 caggatcatt caacgcgcgc ggttgtggaa accaaagatt tcgcggaaaa ggtcaaatgc
     1081 gccgagctgt tcgcgcgcga tagctccaac atcacgcgcg tcaagatcaa tccgggcgcg
     1141 aatgacgggc tggaacccgg tgcggtcacg atcgaggcga cggcggaaga cctgggcgac
     1201 aacacaagca acattgccgc cgccgtcgat ggcgcggaaa tgcaaatcat cttcaacgtc
     1261 aaatatctcg ccgatgttct ctcggtgatc ggtacgccgg aggttgcgct ggaagccacc
     1321 tcgccgacca agccgggcgt gatccgcccc gtcggcgcca gcgattatac gtatgttatt
     1381 atgcccatgc atatcaaccg ttaagccata aggcgggata gatgcatctt tcccacctcg
     1441 tcttgaacga tttccgcaac ttcaagcatc ttgacttgcc cctggggcgc ggcctctcgc
     1501 tgttctacgg tgaaaacgcc cagggcaaga ccaacttgct tgaagctgtt tctatgctcg
     1561 ccacctccaa ttcgtttcac gccacctctg accgcgaggt ggtcaattgg gacgcgcccg
     1621 accgcatcgc gcgtatcatc gcagacatcg agcgcggcga agatgaggtg aaggtagaga
     1681 tcgtgataat cgacgcctcg cccatcgacg cgaacggcga atctgcttat gcttcccaac
     1741 tgcaaacgcc tgatcgccag cgcaaacgca tcaagatcaa cggcggaatt aaaaaggcga
     1801 ccgacctgat tggacaggtg acggtggtgc tcttcgcacc aaccgatttg cgcctcgtag
     1861 acggctcgcc cgaagatcgg cggcgcttcc tcgaccgcgc cctctgccag gtacaaccgc
     1921 gctactgcgc agccctggcg cgctaccgca aggtgattac acagcgttct gccttgctca
     1981 aacgcattcg cgagaaccag gaagacccgc gtatgctgga atttctggac gaaaagctga
     2041 tcgagctggc cagccagatc atctacgagc ggcagcgcat ggtcagggcg ctgaatacgc
     2101 acgtagatgg gttgcaaacg ctcatcagcg gcggacgcga gggtctccag atcgtctatc
     2161 gccccgcatt cgaggttgac cccgcctgga acacactcga tgcgcaggaa cattaccgcc
     2221 gccagctgca cgcggcgcga cggaaagaga ttcagcaggg cgtctgcctg cttgggccac
     2281 accgcgacga cctggacttc atggcgaacg atgtcaactt gctgacctat gggtcgcgcg
     2341 gccagcagcg cacggcggcg ctctccgcca aactcgcgga actggaattc atgcgcgcca
     2401 ccaccggcga ggagccaatt ctgctgctcg atgatgtctt cagcgagctg gacgccgcgc
     2461 gccgcgaata cctgctgcgc ctggcgctcg accacgagca ggcgctggtg acggcgaccg
     2521 atatgaccgg tatcccagaa gagatgctcg cgcagataca ccgcttttct gtcgtcgcgg
     2581 gcgaaatcac tcacacccac ttctgatcga gttcgcgcca ctgctcaccc acagcaatga
     2641 catcgtccac gttgggcaga ctgtcgccgt agcgcaccat cgagtaggct tcggtaatcg
     2701 actccaactg cggctcgacc agcggaacgc cttcatccag gcgctggcgg agttccaggg
     2761 gcgtttcata cttcttgcgc tggtagccgc gaccagcagc ctttttcagc actgcacggt
     2821 atatttcgcg aatagtgcgc accgccgggt cgctggggtg agtcccctgc gcgcccgcca
     2881 cctgcgtttc cgcccggggc ttgtaccgcg agaaacgccc gaagagcgcg cgcaggatat
     2941 tcttgacctg gttccagagc agcagccacg accagaggct ttcgtgaata tctgcatctt
     3001 tccgccggag acgcaatact ttcctgcgcc tgtgctgcac ggcccaccag accaggcaca
     3061 ccagccccag cagcagcaca agtagcagtc ctacacgcag aaacggcagc caggccgcat
     3121 tgatattggt tatcgggtga gtgggcagct tctgcttttg attttgtttg gggatattct
     3181 gcgtacccgg tttaccatgt ggcaacagcg agcctaaaaa ggtaaatagc cagaagatcg
     3241 gcgtcgccag aaagaccagg atatcggcga agatgccgac gagccagttg taggcgttgc
     3301 caatggctat accaacaggc atgaggacgc tcagcgcgct gctgaagaag gtgggatcgg
     3361 cgaacagccc gaccagcagt gtcaccagca gcaggatgat accaagcgag gccaccaccg
     3421 taatcacagc gcgctcttgc gcgacaacgc tgccctgcaa gccgctgaag tgcgatttgc
     3481 gcacgaacac aatgcgcgcc agggcgtgag ccacgagaga cagaaagaga aagacgggga
     3541 tcaacatgaa caggaaaatc gcgtcaggag aagttgcgcc cgaactctcc acggaagcca
     3601 gcagtaggat agcagcgata atcactccca ggcccaaaag tagaccccga atgacctgcg
     3661 atggttcgac tgtgtgactg agcaggcgca gcccgcgcca gcacaggtag cacgaaaaaa
     3721 gcaccatcat gagtgcctgg taaaagtgca gatcgaggaa cagggtatcg ctgcccaatg
     3781 tcgccagcca ggtgagatca tacagcggcc tggtaggagc gtatatttgc aaccagatga
     3841 ggaacagggc cagaacaact agcataacga agaagagcgg catatcctgt ctcacgacgg
     3901 atgcctggcc gtctttgctg ttcttgctgc ctgcctcctg ttcgcttgtt gcctgctgaa
     3961 tgctggcgac gcgccgttcg cgcagcacca gcgtccagtg gaagccgatg atgtagacaa
     4021 atggcgacca gagcggcagc agcggcgtgg agaagccgaa cagatgcgcg ctcgccaggc
     4081 cgatcagggc tgcgcacacc caacacgcct ccattgcgcc cagcagcgtc ggcagcagca
     4141 actcacccag gctggcgatg gtctcttcct cctgcgccag cacatgctgc ctgagccact
     4201 gcgcaggtga cagggccgtc ccgcgccctc tatgcccact cttaccacga tcctccggcg
     4261 gcatctgcat tggttgaatg ggtactggtc tcatggtcgc cttactcagc tcattcggct
     4321 gccgttgcgc ctacgacgaa tgcgtctaca tcgctgattt taccacgcca gtagggaact
     4381 ttgatcttat ggcccgcgct cataattgtc acatcgcgca tatgcgcgat gcggtagatg
     4441 gtaggcaacc cctcgggacc gatctccggc tggtcgcggg gcggaacaag cgcgtaatag
     4501 tattcgcgca gcgcgtcagc cgtttcgggt tcatgctccc ggtgcaattc ctcgttcagg
     4561 tcgatataaa actgggcata gcgcagtgtg ctgaccatca cgccaaacac cagcaggccg
     4621 tgcatctgga gcatcatctc aatctccagg tcctgctgct cgaccagccg cgtccacatt
     4681 tgcaacgcaa aatcctgctt accgtattcc tccagccagc tattgcgccg ttcgatgcgc
     4741 gtcaaggggc gataatccac tttgccggct gcctgcccgt tcgtatgctc ctgttgttcc
     4801 tgtatctcat ccatttagca gcaatcctct aatttggtag catccatcct gttcgaatgc
     4861 atttgtaacc ccattattgt accactgaat ggatatccct cattgtccct gtggagggcc
     4921 acaattgaac gggaaacata ttggcgggcc aatgggttgt ggcggctgcg ggcagccatt
     4981 attatttccg ccattgccgc cattgccacc attgccgcaa ttctgtatgg gaggcgtgta
     5041 gggaccttgc acggtcggca tagtaccatc gatgaaccag tccgtcacgg tcggctcgcc
     5101 ggcatgtggc agcaggccgg tcaaagcgga aactgtcccg gcgtggacat cggaggggcg
     5161 gctaaaatca tcaggcgagt agtggtaata ctgcgaggca tattccatga tgtcatgcca
     5221 gatcggacct gcgccggtga taccgatgac gttctgcatc aggctgttat cgctgttgcc
     5281 agcccagacg ccgacggtca ggtagggcgt gtagcccatc gtccagttat cacggaagct
     5341 gtcggtcgtg ccggtcttcg ccgccgccgg gcgatccagt tcaagcgggt tgccggggaa
     5401 gaattcgtgg tagcgagagg tcttatccga gagaatgctg ctcatcagaa aggccacatc
     5461 cggtcgaagc gcctgcacgc cggcaggatg cgcctggtcg tatttataca gaacccggcc
     5521 ctggctgtcg gtgacttcca gcacactggt ctgcggcaca cgcaccccct ggtcggcaaa
     5581 ggtcgcatag gcccccgtca ggtgcagcag cgagacctcg ctcgcgccaa tcgccatcga
     5641 tggcccaagc tggctcagcg gcttattcgc gatttccgtc agtcccaggc gtcccgccgt
     5701 gttgaccaca ttctggatac cggcaaattc gatggcatcc actgccggga tgttgatcga
     5761 attggcgatg gcattgcgaa tggtcatcgg gaagccagag aggtacgtgt tgccgtaatt
     5821 gttcggcgaa tagtattgca cgggattcga cgaaatcaac gtcgggtagg tggtcttatg
     5881 gttccctagg atcatggcgg gataccagcc catctcaaag gccgtcgcgt agacaatcgg
     5941 cttgaaagac gaacccggct ggcgcagcgc ggtggccgcg ttgaaatgtc cgctcacctg
     6001 cggtcggctg ctgttgtagc ttgcgctgcc atccatcgcc agaatttccc cgttgaaggg
     6061 gttttcgacc accaccgccg cgttattgac gttattcgtc gtatccagcg gcccataaca
     6121 gcccaggtag ttatcgcact gctgggtgta gagatggttg tagacgctct tctcgacata
     6181 ctgctccagg tgcaggtcca gcgtggtgta gatgttgtag ccgccatcgt agaggttctg
     6241 cgcgccaatc atgggggcca gcacgtcatc gatgacgtac tgcacgaaat ggggggcctt
     6301 gatgccctgc ctggagctat aggactgaaa gttgaacttc gctgtttccg cctcagcctg
     6361 cttcgcctgc gtatccgtaa tcatgccgag gtcgatcata gattgcagca catacttctg
     6421 acgatccagc gcgactgctt tgttctcgat gggattgtaa taggtgggaa gctgagggag
     6481 gccggccagc agcgacgcct gggccaggtc aagctgggag gccgccgacc ggcaagtctt
     6541 ctgggtacat ttcggctgaa tgttgaagta atcctgcgcg gcagcctcga tgccgtaatt
     6601 aaggtcgcca taataaacgg tgtttagata catctccatg atcttccact tgggatattg
     6661 ctgggtcaga ccataggcca ggatggcttc ctcacccttc acctgcaatg ttctgggatc
     6721 gttcgcgaat aactgctgtt taatcaactg ctgggtgatg gtgctgccgc cttccaccac
     6781 tgtctggttt tgcaaattgg tgacggcggc tcgcagaatc ccctgcacat caatgccgct
     6841 attcgaccag aaggtacggt cttcagccgc gacggtggcg ttgacaagat tttgcgaaat
     6901 ctcggtataa ttcacatagg tgcggcgtcc gtagcgcgga tcattgagat caaaaagcag
     6961 gttgccgttg cgatcataga tgtgcgtggt ctgaaacagc gagtggctgg cgatattgtc
     7021 cagcaggggt agctgtgcct tgtaatatgc gaatgcgcca ccggcagcgc cggataacag
     7081 cgtgataagt atcgcactca ggacgagcaa caaaatcatc agacgcggcc cggtctgctc
     7141 gctgcgctcc tggcgggtat gcctgcgctt gatgaacatc acatggttga cacgcgctcg
     7201 ccttcgcgcc atctctggcg ctatcgctgc gagcgggccg ctcaccgggc gtgggactgg
     7261 agagatctga gtggcagacg cagcgcgcga caaagtttca tcgctcaaaa taatatcttg
     7321 ttcgtccata ctgtatcttc gaggctccct ctttagtata gcctaccatc gtgtatcata
     7381 ccacatcttc atcaaacgct tgacttgttt gcaatatatc ggtacatttg gtataggaga
     7441 tgtgagaagc aagtagtgcc acactggtat aacgcttatg tgcgcctgga cgtttcgttc
     7501 attgcgccat gcaactcttg tatccgcagt tcgtacttct tacagaggga gtatctctct
     7561 cacaaggatg tgtcgttgcc ctgaaatggg gctaaggagt ccctaatgag tcaacaagaa
     7621 tttgttccca ggtcgcagca aaacccaccg cctgagaacg aagaggaaat atacgagcca
     7681 cagcacccgt attcctggtc cgggaagagc aaatctgagg cggagccgcg agacgagccg
     7741 ccgtcgagct acgaatatga agctggctac caggctcaag atgcttatga ccagaatgct
     7801 tacgagcatg aagcgccatt cgacccggcg aactcctata caccgttggc tgaacaggct
     7861 ccgcaggtac agcaacaaca gcagtaccag aacccttacg acgccaatac ctatgggcag
     7921 aattcctatg ggcaggacta caatgcttac aacaattaca aatccgcgca gagcaactcc
     7981 taccagaacg ttccgcccta cgcccgcccc cagcgtcagt ctggcggcag tccctggcgc
     8041 tttagcagca tactgctcat actcgttctg atcaccattt tcagttcagt tggtcgtgac
     8101 tttttcttct acggttttca tgggtttgat atgattatcg ggtttttccc gattcttatc
     8161 ctgttgctca tcttcaacgg gattcgtaga ggtgggcgtc gtcgcttccg gcgcggcccc
     8221 tggggatggt aatagtcgtt gagtgagatt gaacaagttg atcgacgcgc ccgcttctca
     8281 cgcggcattc gcgacgatgt agacctgacc gatatccatg agctgcgcag cctgctgtac
     8341 gctcatcata tgcgcccaaa caaatcgttt gggcaaaatt ttctggtgga ccgcgctatt
     8401 ttacagcagg tcgtagatgc cgcccaactt gagccaggcg accaggtatt ggaagtcggc
     8461 gcgggaactg gcgtcttgac acgcgagctg gcgaagcagg ccaggcgtgt tgtggccgtc
     8521 gagatcgaac gcgatatgat cgagctattg cgcgaaacaa cgggccatta cccgcatgtg
     8581 gagctgatag agcgcagcct gctcgcgctt gatccctgcg aagtgttcga ccaggagccg
     8641 tataagctgg ttgccaacct gccctattac attaccgctc caacctttcg ccacttcctg
     8701 gaaagccgca atccaccgcg cctgctggtc gtgatggtgc agcgcgaggt cgcgcagcgc
     8761 atcgtagcag ggccgggcga tttaagcctg ctgggtgtca gcatacagtt ttacggcaag
     8821 ccggaactgg tcgcccatgt accggcgcgc gcctttttcc cttcgcctct ggtcgattcc
     8881 gccatcctgc gcgtggaggt tcacgaccag ccacctttga cacgtaagga acgtgacagt
     8941 ttttttcgcg tcgtccaggc tggcttcgcc gaaaaacgca aacagttgca taatgctttg
     9001 acccatcatc tacatcgcaa aaatgaagat attcgcgcct gtctcgtcgc cgcgaacatc
     9061 gatcctggcc gccgcgccga aacgctctcg atcgaagagt ggctgcgctt atggaacata
     9121 ttgcagcagc caaaaacaag cttataatga tatttgttga ggcaaacagg caacgttttc
     9181 gcgtatagta taatatgcgt gagaagctat tacctgatag acagagattt gtatggaaga
     9241 agttgaagac ctggaagaac aacagcaacc tgccacacca cctgccttga ccggcgatac
     9301 tgacgataat acagtcaaaa cggacatcgg gtcggaatca tccggtagtg ctgatacaat
     9361 cgccccggag gaatcgccga cgcagacgac gctggccgac gaaatcgccg ggcaagacgc
     9421 gccaacgact caaccggttg gggtggatgc gacaaccgag gcactctttt gcgatgactg
     9481 cggcgcggcg aatcctgccg ggtcgcgttt ctgccagcat tgcggctcgc tgatcccttt
     9541 tcagcgccac accgggacgc tgcctatgca aatcatgctg gcgggtcatt atcagttgct
     9601 cagccgcatc ggccagggcg gcatgggcgc ggtctacaag gctgccgaca caaagctcaa
     9661 caatcgctcc gtcgccatca aagagatgaa tcgttcgggt ctgagttcca ctcgcctgaa
     9721 agaagctgag gatgcatttg agcgcgaggc gcacctgcta gctgatcttg tgcatcctaa
     9781 tctgccgcgc attcacgagc atttcacgga aaacgaacgc tcgtacctgg tcatggattt
     9841 tatcgaaggg caaaccctgg aggagtacct ggagaaaacc ggcggcggtc cggtgccagt
     9901 ggacgtggtt gtacgctggg ccacagaact atgcgacgta ttgaattacc tgcatacgcg
     9961 ccagccgccc attatttttc gcgatctcaa gcccgccaat gtcatgatta gcgagaccgg
    10021 ccatatcttc ctgatcgatt tcggtatagc gcgtctcttc aaaccgggca aacagcacga
    10081 cacggtcgtt ctcggtacgc ctggctatgc cgcgccggaa caatacggca aaacacagag
    10141 cagcccccgc tccgacctct acagcctggg cgcgttattg catcatctcc tgaccggcat
    10201 cgatcccagc gaacacccgt ttttcttcag cccggcctcg caggtcaacc cggctgtcaa
    10261 ccctatgctt gagcgcttgc tcaaacagat ggtggagatg gatagcggga aaagacccac
    10321 cagcgcgcag gaggtcttgc ggacattgga gagcgctacg acgagaccaa cttctgccat
    10381 gctcgccaac acctcttcta cgatgagtat aactgatccc ttgcttgagc aggcctatcg
    10441 cgcctattca cagaagcgcc tggacgaagc gctgaaacta tacagccagg cgctgcaaaa
    10501 ggatagctcc agctccctcg cctggcaggg ctatgggctg acgcaggggc tgcgcgcgca
    10561 gcatcaagat gcgctgacct cctttgaacg cgcgctgcaa ctgaaccccg gactggtcac
    10621 gtcctggaac ggcaagggga cagcgctcag tcgcttgcgc cgccatcagg aggcgctggt
    10681 cgcatttgaa cgcgccgtcg aactcgatcc gaataacgcc gccgcctgga atggcaaggg
    10741 cgcgacactg aacgccctgg gacgcgcggc gctggccctc gatgcgttcg atatgtcgat
    10801 cagccatgat ccgcaattaa ttcaggcatg gaataataag gggctggttc tctacgaact
    10861 tgggcgcttc aaagaagcgc agaaagcctt cgaggaggcg ctcgacctgg atagccacaa
    10921 cgcccacgcc cttttcggca agggccaggc gctctacggc cagcgcaaac tgaagcaggc
    10981 gtttactctg tacgaacagg ctctgcgcgt cgatgatacg tttgccgatg cctggaaccg
    11041 gcgcggcaac gtgcttgacg agatgggcga caatggcgcg gcgctccaat cgtacgatcg
    11101 cgcgctccaa ctcaacccac gcctggtggc cgcctggaac ggcaaggcgg gtgtgctgcg
    11161 caagttgaaa cgtctcttcg atgcctttac cgcctacaat cacgccctcg atatcgatcc
    11221 aaaattcgcc ctggcatgga atggtaaagg caataccctc ttcgacatga agaactacgc
    11281 tgaagccctg ctctcctacg agcgcgcgct gcaatataac cagcgcctgg cgaccgcgtg
    11341 gcataataag tcgctggtgt tataccgcct gggtcgctat gatgaagccc tgcgagccgc
    11401 cgacgaggct attcacatgc gccccgatga tcccgacaac tggcagcgca aggctgatgc
    11461 cctcaaagcg ttgaaccgcc gccaggaagc cagcttcgca gagcggcagg tcatctacct
    11521 gaaaggtcgg gcgtaactac ccgcctgcgt ccacggtaat ctgcaaattc acgcccggcg
    11581 cgctcgtgct ggtaacagcg tatgacccgt acagttcaaa gactacatca accgacgcgc
    11641 cgggggccag ccggatgccg ggagacttca acacgacagg tgcgacggcg ctggtgtcgc
    11701 acgttctatc ctggcccaga catattgtct gcatgacatt cgattgattg acaatgtgga
    11761 tcgtctcccc ttcggtaatt tcaacggtgc tgcgcgcaaa gccactggtc gtcacatcta
    11821 cctcgaccag cgcatggaag cgcaggtaca aaaatactac cagcacaatt gctaccgcga
    11881 acaggagcgg cgggatgaca agatagacta ctattttctt tgcgcgagac attttcatcg
    11941 ttcactatcc ttgttcaaat gatgtgtagg ggagcaattc attgcgccct cttcctctcg
    12001 gtcaagcgct tccaccaggg cgcaatgaat tgctccccta ctagtgtggc atttcccaaa
    12061 gtgactcctg atgccagagc tgtgtcgagt cccattcgca gcccagaccc ggtggcaaca
    12121 ccgagagatg cacctgcgcc ggatttgcct ttgcctcggt gatgcgcgtg cgcatactgt
    12181 ccagagccaa cctgggcgta ttgttggtac ggctgaggtg cgagagccag agccagcgca
    12241 caccgtcgct gcgccaggta cgcaataccg ctccggcggc ctggtcattg gaaaggtgcc
    12301 ccgatgggct gagaatacgc tgtttgagat gatagggata cggaccacgc agcaggcgct
    12361 cccggtcatg attcgactca agcaccagca aatcagcttg attgattgcc tccagcatgg
    12421 catctgtgac ctcgccggta tcgattacca cgcagacgcg acatccaccc gcgctcaaca
    12481 ggtagccaca gggcgcaacc gcatcgtgtg agacgggaaa cgagcgcacc tcaatatcac
    12541 caatcatcct gcgcgagcca accgggaaag cgctcgaacc atgttcgatc acatctggag
    12601 catcatcgat gcctgtctcc atctccggtt cgtcagcggt gatatttgcg tcgtcgggaa
    12661 ggacggcggg cgacgcaagc gtcccctctc cacccaaccc tgcccccgcc cttacggatg
    12721 ggtatgttag ggaggggaat ggagcggaga gggatgaata ggcgagcgtc ttttcgatag
    12781 cagccagggt gcgcgggtcg gctaaagccg gtattttgta gcgctttact aagaagggca
    12841 ggccgataat atggtcggta tgctcgtgtg tgacgaggat gccggaaagc cgcgccgggg
    12901 acactccggc ttgccgcaga cgttccacga ggatgcggcc aggaaaaccg gcgtcgatca
    12961 agatgcttgt acggcctcgc ggcccggctt cgatgaacaa agcgttgccg ctgctgccac
    13021 tgcccaatga cacaacacgc atagattata gccctaataa ttgtcgcgca ttctcgccca
    13081 gaatgcttct caccgaggtt tcatccaggc ccgactgcct gatatggtcg attactcgct
    13141 tttgacgcag cagaccgtag tcactggcga acagaatgcg gtccgcgcca acgagcgcga
    13201 ccatcgccgg aaaaatacgc gcgccatata aatagattgt agcagcagta tcataccata
    13261 cattagtcgt gatggcctgt atctccggca tcaaggtata gaaaggaagg ccaccgcccc
    13321 aatgcgcagc aatgaagcgg atatcgggga aggcgctcaa aaacgcgaca atatcgggca
    13381 acgagacatt acccttgccg ggataaaggt gtccaaccgg ttcgctgcaa tgcgccagga
    13441 cgagcaggcg atagcggcgt accacctcca tcagcggcgc gagcaaagga atattgctca
    13501 gttggtagcc ctgaccatgc ggcatgagtt cacccacacc gatcatccct gcctgcgcgc
    13561 atcgctcgag ttcgtgtatc gcctggtccc cgctcgtggg taagcagaca gacatgccat
    13621 agattcgacc tgggtaacgg cgcatggcat cgatgacata atcgttggtt tctacgatca
    13681 acgcggggtc tgtccaacca aacgagaagg tgaccgctac atcaactcct gttgcgtcca
    13741 tctctgctac aaggtcatca gcgctggcga taactgcacg tgacttgcgg taaagctcgc
    13801 cgaaccagcg atctcgttca caataatatt cgcgacgccg gacaatgtcc ggcgggaaaa
    13861 tgtgcgtatg aaaatcgatc agcattgcca tcacataatt cgtactaata tagaaaaggc
    13921 gcaatacgat gtattgcgcc tactgaaaaa cattattacc tcaattttgc cgagaccagc
    13981 cgagggacca cgagcgccga gtcaccacag acgcgttcgt cgaccctgac ccgcgctccc
    14041 ggcgcgggat acaagccgtt gggatatgag ccccgcttgg ctgcgccggt taccc
//
LOCUS       DASZAK010000136         1705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_110050, whole genome shotgun sequence.
ACCESSION   DASZAK010000136 DASZAK010000000
VERSION     DASZAK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110050"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VGT44_02385"
     CDS             <1..494
                     /locus_tag="VGT44_02385"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579675.1"
                     /translation="SGIEALLLLGRTAAHMQALLDHLRALAAAMNHPPSIMGALLPAK
                     QQGQQFMLYNESLSGGDETMWFLSSADVLVNATSVGLKADDADTTRLLIDVNALPATT
                     LVMDMIFNPPQTALLRAAHARGCATLNGLSMLLYQGALAFELWTGRAAPIEAMRKALG
                     IFL"
     gene            517..1686
                     /gene="aroC"
                     /locus_tag="VGT44_02390"
     CDS             517..1686
                     /gene="aroC"
                     /locus_tag="VGT44_02390"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV2579676.1"
                     /translation="MFRFLTSGESHGPGLTAIIEGIPAGLPLAVEQVNYHLQRRQKGY
                     GRGGRMKIESDQVTLRSGMRHGLTLGSPITLEIENRDWQNWQVKMSVTPVDEAVDPVT
                     LARPGHADFAGAIKYGHDDIRNVIERSSARETAARVAVGAVCQVLLQQFGVEIHSHVH
                     SIGPVGGRDAASSAIGNEYTAAYWEQVEQSQVRCGNSELEARMIDYIHECKARGETCG
                     GVFEVIATGVPIGLGSYSQWDRRLSAKLAMAIMSIPSIKGVEIGGGFEMSALPGSQVH
                     DVARWNDEQGWSHRSNNAGGIEGGISNGAPIIARGAVKPISTLAHPLPTVDIRTRENV
                     AEGRYERSDVCVVPAAGVVGEAMMAIALTEAWLEKFGGDSMAEMKANYEAYVDRM"
BASE COUNT          331 a          454 c          606 g          314 t
ORIGIN      
        1 aaagcggcat cgaggcattg ctgctacttg ggcgcacggc ggcgcatatg caggcgctgc
       61 tggatcacct gcgcgcgctg gcggcggcga tgaatcatcc ccctagtatc atgggcgcgt
      121 tgctgcctgc aaaacagcaa ggtcaacaat tcatgttgta taatgagagc ctgtcaggtg
      181 gggatgagac gatgtggttt ctctcctcgg cggatgtgct ggtgaatgcc acatctgtcg
      241 gcttgaaggc ggacgacgcg gatacgacgc gcctgctcat cgatgtcaac gcgctcccgg
      301 caacgacgct tgtgatggat atgattttta acccgccgca gaccgcgctg ttacgcgccg
      361 cccacgcgcg cggctgcgca accctcaacg gcctatcgat gctgctgtac cagggcgcgc
      421 tggcctttga gttgtggacg ggccgtgccg cgccgatcga agcgatgcgt aaggcgttgg
      481 gtattttttt gtaggtgtat aaggatagtc aaggatatgt ttcgatttct cacgtcaggc
      541 gaatcgcatg ggcccggctt gacggctatt atcgagggta ttcctgccgg gctgccgctt
      601 gcggtggagc aggtcaatta tcatctgcag cgcaggcaga aggggtatgg gcgcggcggg
      661 cgtatgaaga tcgagagcga ccaggtgacg ctgcgctctg ggatgcggca tggtctgacg
      721 cttggttcgc cgatcacgct ggagatcgag aaccgcgact ggcagaactg gcaggtcaag
      781 atgagcgtga cgccggtcga tgaggcggtt gatccggtga cgctggcacg accgggacac
      841 gccgatttcg cgggcgcgat caaatatggg catgacgata ttcgcaatgt gatcgagcgg
      901 tcgagcgcgc gcgaaacagc cgcgcgcgtg gcggtcggcg cggtctgcca ggtcttgctg
      961 caacagttcg gggttgagat tcacagccat gtgcattcca ttggacccgt tggcgggcgt
     1021 gacgcggcca gcagcgctat cggcaacgaa tacacggcgg cgtactggga acaggtcgag
     1081 caatcgcagg tgcgctgcgg caacagcgag cttgaggcgc gcatgatcga ctacatccac
     1141 gagtgcaagg cgcgcgggga gacctgcggg ggcgttttcg aggtgatcgc gacgggcgta
     1201 ccaattggcc tggggagtta cagccagtgg gaccggcggc tgagcgcgaa gctagcgatg
     1261 gcgataatga gcatcccatc catcaaaggc gttgagatcg gaggcggctt cgagatgagc
     1321 gcgctgcccg gctcgcaggt gcatgacgtg gcgcggtgga acgacgagca gggctggtcg
     1381 caccgctcga ataacgcggg cggcatcgaa ggcggcatca gcaacggcgc gcccatcatc
     1441 gcgcgcggcg cggtcaagcc tatctccacg ctggcgcatc ccctgccaac ggtggatata
     1501 cgcacgcgcg agaacgtggc cgagggccgc tacgaacgca gcgacgtatg cgtcgtcccg
     1561 gcagcgggtg tggtaggcga agcgatgatg gcgattgccc tgaccgaagc gtggctggag
     1621 aagttcggcg gcgacagcat ggcggaaatg aaggccaatt atgaggcgta tgttgatagg
     1681 atgtgaacgt ctgcgcgagg catgg
//
LOCUS       DASZAK010000137         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_180266, whole genome shotgun sequence.
ACCESSION   DASZAK010000137 DASZAK010000000
VERSION     DASZAK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180266"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VGT44_02395"
     CDS             complement(<1..173)
                     /locus_tag="VGT44_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV2579677.1"
                     /translation="MWLQVSTKGEYGVRIMVDLARHYGEQPRSLTEIAQAEMLPLAYL
                     EQLAKLLREAEPPL"
     gene            complement(279..1085)
                     /locus_tag="VGT44_02400"
     CDS             complement(279..1085)
                     /locus_tag="VGT44_02400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2579678.1"
                     /translation="MSYHLGEYYERSDDYDLEYRSQSEQDVPFWRELVMRYTPQRVLE
                     LACGSGRIGIELLHGPGSFQLEGLDISPDMLAAYRRKLEREPESVQQRVTLHEADMVD
                     YLLPNKGTFDLALLPFNSVSHLYEIEQQLSAFKTTCDHLAPGGRFVVDIFVPDIDYLS
                     DALNRPTQVYLEDEIEVANEFTMLLYTARKYDPIEQLQHILFTHEKFFESGDHERYLT
                     KLDMHMFFPRELQLLFLATGFRVEAIYGDYDWRAFGAGTRQIIVGRKSAS"
     gene            1246..>3999
                     /locus_tag="VGT44_02405"
     CDS             1246..>3999
                     /locus_tag="VGT44_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2579679.1"
                     /translation="MPTAIWGESEPRSDDVYPLSLLKQLLAQLMERFSAQGACLALYD
                     EHTRQMVIRLHRHAPSAASAQMEIAPGATGSDIDQIDTIPIVRRKTAGLSSPATPTSG
                     TGRMRRLTRPLSSLDRLSDSSPAALFPLGEAYGPGQGLIGITWRKGEPLSFPREELTL
                     PTRGQGGNVDALPTWYLSLPIQGPLLPTMPASPVPATSPMPPENQPGSQAGQREHSGQ
                     SGQPPFQALGVIVLYQYAPAQGFDQQQIEEALQYAERIGLYIQNDHLRRAQADMYNYI
                     QRLQQISTVFPSNVLLSRLVEDVYRFVMAVVPASSVLMTLFDRDTKKLYDVFAVDHGQ
                     PITGLADHPVVADPALRPVWQRIAEKEQHTLLLSTSEEDAAVFERYGELLHGTWGDQT
                     GSASFLLLPMKMFTRVVGSLSISCKLPGAFTPEKILVLETMAQLITVSIENTRLYDRS
                     SRMLQKAKQHELSLAAMNSSLLTISTVLNLGELLQKFVEMAARMVEAELCTFFQLTKE
                     GDELVAQAVYDQTGRWRKTVENAMQVSSEESHSNLIQMIRLPFLQSMLPALIESESFF
                     YLDSTTIDELIAASSEGGAAFLRETHTHRLLMIPMRYQTELMGILAVHTPSHDHRFRP
                     EGVSTLQAISAQAASAIRNAQLFEQITQANLELRRMDKLKDEFIVTASHELRTPLSAV
                     SGYASLLKRSSSRINQQQVLRYATKIGDAAQQLVALVNSMTDAARLGALDGKLDLKLG
                     PVQLAVATTVAMEMVSINIEQKISVQVDPDLWVYCDAMYLRQVLSNLLDNAAKYSPPT
                     GQIIVSAAATWLSQLPEKQVNYQKLVNEGDQEMALVHVCDEGDGIPPEEQEKIFEKFV
                     RAPRSLTTPIRGSGLGLYMCRHYVEAMGGKLWLEVSIPGEGSIFSFYLPRISAPPLEK
                     EPHE"
BASE COUNT          807 a         1090 c         1231 g          871 t
ORIGIN      
        1 aggggcggtt cagcttcgcg caacagcttt gccagttgtt ccaggtaggc cagcggcagc
       61 atctccgcct gggctatctc ggtcaacgag cgtggctgct ctccataatg acgggcgaga
      121 tcgaccatga tgcgtacgcc atattcgcct ttcgtcgaga cctgtaacca catggtcgtt
      181 ctcccttcct gtaaattggg cttgccagcc gcgcgtcatg cgcgaattaa tccgacctaa
      241 tcactaggat atattttagc actatggagg ggagagcgtc aactggccga ttttcgtcct
      301 acgatgatct gccgtgtgcc tgcgccgaag gctcgccagt cgtagtcgcc gtagatggct
      361 tcgacacgaa agcctgttgc caggaagagc agttggagtt cgcgcgggaa gaacatgtgc
      421 atgtcgagtt tggtaagata gcgttcgtgg tcgcccgatt caaaaaactt ctcgtgtgtg
      481 aaaagaatgt gctgcaattg ctcgatgggg tcgtatttgc gggcggtata gagcagcatg
      541 gtgaactcgt ttgcgacctc aatctcatct tccaggtaga cctgcgttgg acggttaagg
      601 gcgtcgctga ggtagtcaat atcgggtacg aagatgtcca ccacgaagcg gccacctggg
      661 gccaggtggt cgcaggtggt tttgaacgcg ctgagttgtt gttcgatttc gtagaggtgc
      721 gagacggaat tgaagggcag cagcgccagg tcgaacgttc ccttgttggg caacaggtag
      781 tcgaccatat cggcttcgtg cagtgtgacg cgctgctgga cgctctccgg ctcgcgctcc
      841 agcttgcgcc ggtaggctgc cagcatgtcg ggcgagatat ccagcccttc gagctggaag
      901 ctgccggggc cgtgcagcaa ttcgatgccg atgcgcccgc tgccgcaggc cagttccagc
      961 acgcgctgcg gcgtgtagcg catgaccagc tcacgccaga agggaacatc ctgctccgac
     1021 tggctgcggt attccaggtc gtagtcgtcg gaacgttcgt agtattctcc gaggtggtag
     1081 gacataggtg ttctctttta agctttcact tccatttgta tcgatgagat tatagcatgg
     1141 ctgtgttata agcgtaaaga agtggttgag ctaccgaact ccaacatggt tgtgatatac
     1201 ttagcgctat cattagccgc ttgagacacg aggtataagg aacggatgcc tactgctatc
     1261 tggggtgagt cggagccgcg ttcggacgac gtttatccct tgtcgctgct caaacaactg
     1321 ctggcgcagt tgatggagcg attttcggcg cagggcgcat gcctggcgct gtacgatgaa
     1381 catacccgcc agatggtcat tcgcctgcac cggcacgcgc cttctgctgc gtccgctcag
     1441 atggagatag cgccgggcgc aacgggcagt gacatcgacc agattgacac gattccgatt
     1501 gtgcgccgca aaacagcggg gctaagcagt ccggcgacgc ctacttcggg aacaggaaga
     1561 atgcggcgtt tgacgcggcc attatcgagc ctcgaccggc tgtcggactc ttcaccggcg
     1621 gcgcttttcc cacttggcga ggcgtatggc cctggccagg ggctgatcgg cattacgtgg
     1681 cgcaagggcg agccactgtc attccctcgc gaagagttga ccctgcctac caggggtcag
     1741 gggggaaatg ttgacgcgct tcccacctgg tatctctcgc tgccaatcca ggggccgttg
     1801 ctgccgacca tgcctgcctc gcctgttcct gctacatcgc cgatgccgcc cgaaaatcag
     1861 ccgggcagcc aggcgggaca gcgcgagcat agcgggcaat ccgggcagcc gccttttcag
     1921 gctcttggag tgatagtact ctatcagtac gcgcctgcgc aggggtttga ccagcagcag
     1981 atcgaggaag ccttgcagta tgccgaacgc atcgggctgt acatccagaa cgatcacctg
     2041 cggcgcgcgc aggcggatat gtataactac atccagcgct tgcagcagat tagcacggtt
     2101 tttccttcga atgtcctcct gtcgcgactg gtggaagatg tctaccgatt cgtgatggct
     2161 gttgtgcctg cctcctcggt gttgatgacg ttattcgacc gcgatacgaa gaaattgtat
     2221 gatgtctttg ccgtcgatca tggtcagccg atcacggggc tggccgacca cccggtggtg
     2281 gcagaccctg cgctgcgacc ggtgtggcag cggatcgcgg aaaaggaaca acatacattg
     2341 ctgctctcaa ccagcgagga agatgccgcg gtgtttgaac ggtacggcga actgctgcat
     2401 ggcacctggg gcgatcagac cggctcggcc agcttcctgt tgctccccat gaagatgttt
     2461 acgcgcgtgg ttggctcgct ctccatttcc tgtaagttgc ctggagcgtt tacgcctgag
     2521 aaaattctcg tgcttgagac aatggcgcag ttgattactg tcagcatcga aaacacgcgg
     2581 ctctatgatc gctcgagccg tatgttgcaa aaggccaagc agcacgagct atcgctggcg
     2641 gccatgaaca gctcgctgct gacgatcagc acggtgctga acctgggcga gttgctgcaa
     2701 aaatttgtgg agatggcggc caggatggtg gaggccgagc tatgcacctt cttccagctt
     2761 acaaaggaag gcgatgagct ggtggcgcag gcggtctatg accagacggg caggtggcgc
     2821 aaaaccgttg agaacgcgat gcaggtcagc agcgaagagt cgcatagtaa tttgattcag
     2881 atgattcgcc tgccttttct tcaatcaatg ctgccggctc tgatcgagtc agagtccttc
     2941 ttttacctgg actccacgac gattgatgag ctgatcgcgg cgagtagtga aggcggggcc
     3001 gcattcttgc gcgaaacaca tacgcaccgc ctgctgatga ttccgatgcg ctatcagacc
     3061 gagcttatgg gcatcctggc ggtgcatacg ccctcgcacg atcaccgctt ccgcccggag
     3121 ggtgtcagta cgttacaggc tatttcggcg caggcggcaa gcgccatacg caacgcgcaa
     3181 ttgttcgagc agatcacgca ggccaacctg gaattgcggc gcatggataa gctcaaggac
     3241 gaattcattg tgacggcctc gcacgaattg cgcacgccgc tcagcgctgt cagcggctac
     3301 gcctcgctgc tcaaacgctc cagttcgcgc attaaccagc agcaagtcct ccgttatgcc
     3361 acgaagatcg gcgacgcggc gcagcagctc gtcgcgctgg tgaattcgat gacggatgcc
     3421 gcgcggcttg gggcgcttga cgggaagctc gatctcaagc tggggccggt gcagttagcc
     3481 gttgcgacaa cggtggcgat ggagatggtt agcatcaata tcgagcagaa gatttccgtg
     3541 caggtggacc cggacctgtg ggtctactgc gatgccatgt atctgcgaca ggtgttgagc
     3601 aacttgcttg ataatgccgc caaatattcg ccgcccacgg gtcagatcat cgtcagcgcg
     3661 gcggccacgt ggctttcgca gcttcccgaa aagcaagtca attaccagaa gctggtcaac
     3721 gagggcgacc aggagatggc gctggtgcat gtctgcgacg aaggcgacgg cattccgccg
     3781 gaagagcagg agaagatctt cgaaaaattt gtgcgcgccc ctcgctccct gacgacaccg
     3841 attcgcgggt cagggctggg cctgtatatg tgtcgccact acgtcgaggc gatggggggc
     3901 aaactgtggc tggaggtcag tattcctggt gagggttcga tcttcagctt ctatctgccg
     3961 cgtatctccg cgccgccgct agagaaggag ccgcatgag
//
LOCUS       DASZAK010000138         2472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_358421, whole genome shotgun sequence.
ACCESSION   DASZAK010000138 DASZAK010000000
VERSION     DASZAK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358421"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /locus_tag="VGT44_02410"
     CDS             complement(<1..1538)
                     /locus_tag="VGT44_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV2579680.1"
                     /translation="MPIVNVVQVEKSYGADLVFSGVSFQIQEQDRIGLIGPNGEGKST
                     LLNLLAGREEPDEGTLAVGRTVRIGYLTQLIDFQPENTLLEEMLTVFDQVRAWDGKLE
                     ELAQAISASATDSEEAIHEELMQQYAELQQRFEHAGGYTYEYKVSQVLDGLGFTREQQ
                     AAPVKRLSGGQQTRAALGKLLLQEPDLLLLDEPTNHLDLATMEWLESYLAGWKGAMVV
                     VAHDRYFLDKVVSRIIEMAFGRIEEYPGNYTQYLRLREERMERRMREYEAQQAHISHT
                     EEFIRRYKAGQRSREARGRQKLLDRLDRVERPQEFQAMHFEFNTVVNSGQVVLSTQNL
                     AVGYPASQPGSAANVLMRVADLEVLRGDRIGLIGPNGAGKTTLLRIITSKLAPLNGQV
                     YPGHNVRIGYYSQTHEGLNLDRTIIDEIRQVSVLSEEGARGFLGRFLFSGDDVFKPIG
                     ALSGGERSRVALAKLTLQGSNFLVLDEPTNHLDLQSRQFLEEVLGDFEGTLLVVSHDR
                     YFIDR"
     gene            1892..>2472
                     /locus_tag="VGT44_02415"
     CDS             1892..>2472
                     /locus_tag="VGT44_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEV2579681.1"
                     /translation="MAKEQSLDNLWLAQALYELGGVQFGNFTVSESAVSSPVFVNPKV
                     LISNPTALRVASKLMQQEINLAQSLRRPRVHPFGFVAGVPVGGLLLATAYALETNIPL
                     IYARTRPEGTGKRGIEGRYTRGETVLVIDDLITRGSSILETAELLEESGLKVKDVIVL
                     IDREHGAVERLRRHNYNLISILKLDVMLTHYLSR"
BASE COUNT          534 a          742 c          658 g          538 t
ORIGIN      
        1 cgatcgatga aatagcggtc atgcgagaca accagcaacg tcccctcgaa gtcgcccagg
       61 acttcttcca gaaactggcg cgactgcaga tcaagatgat tggttggctc gtccagtacg
      121 agaaaattgg agccttgcaa cgtcagttta gccagcgcga cgcggctgcg ctcgcccccg
      181 ctcaacgcgc cgatgggctt gaagacatca tccccactga aaaggaagcg tccaagaaaa
      241 ccgcgcgcgc cctcttcgct caagacgctg acctgtcgga tttcgtcgat gatggtgcga
      301 tccaggttca agccttcgtg tgtctgcgaa tagtagccga tgcgcacgtt gtggccggga
      361 tagacctggc cattcagtgg agcaagcttg ctggtgatga tacgcagcag cgtcgtcttc
      421 cccgcgccgt tcggcccgat caagccaata cgatcgccgc gcaaaacttc caggtcggcc
      481 accctcatca gcacattggc tgcgctgccg ggctgcgagg caggatagcc cacggccagg
      541 ttctgcgtgg aaagcacgac ctggccgcta ttgactactg tgttgaattc gaaatgcatc
      601 gcctgaaatt cctgcgggcg ctcgactcga tcgaggcgat ccagcagctt ctgtctcccg
      661 cgcgcttcac ggctgcgctg gccggctttg taacgacgga taaactcctc ggtatggctg
      721 atatgcgcct gttgcgcttc gtattcgcgc attcgacgtt ccatgcgctc ctcgcgcaag
      781 cgcaggtatt gggtataatt ccccggatac tcctcgatgc ggccaaaggc catttcgatg
      841 atgcgcgaga cgaccttatc gagaaagtag cggtcatgcg ccacgacgac catcgcgcct
      901 ttccagcctg ccaggtagga ctccagccac tccatcgtgg cgagatcaag gtgattggtc
      961 ggctcatcca gcagcagcag gtcaggttcc tgtaagagca acttgcccag cgcggcgcga
     1021 gtctgctgcc ctccgctcaa gcgcttgacc ggggcggcct gctgttcgcg cgtgaagccc
     1081 aggccatcca gtacctggga tactttgtac tcataggtgt agccgcccgc gtgttcaaag
     1141 cgctgctgca actccgcgta ctgctgcatc aattcctcgt ggattgcctc ctcgctatcg
     1201 gtcgctgatg cgctgatggc ctgcgccagc tcctcaagtt tgccatccca cgcgcgcacc
     1261 tggtcaaaga ccgtcagcat ctcttcgagc agggtatttt ccggctgaaa atcgatgagc
     1321 tgcgtcagat agccaatcct caccgtgcgc ccaacggcga gcgtcccctc gtccggctcc
     1381 tcccgccccg ccagcagatt aagcaaggtc gatttcccct cgccattcgg tccgatcagg
     1441 ccaatgcgat cctgttcctg tatctgaaag ctcaccccgg aaaagaccag gtcggctcca
     1501 tacgatttct caacctgtac aacattcaca attggcatag cgactcacct gtctgtaaac
     1561 gattatgcag ttttacgata taatcagggc tgcgccccta cggacacttc ttggctgttg
     1621 ttcgtccaat cattatgaat tttaccgacc aaagtcggaa atgtcatcct gagcgcagcg
     1681 aaggatctcg cggcccatca actgagaccc ttcgctgcgc tcagggtgac aatcgatgac
     1741 tggcggtgaa agttcataat gaatctgccc gccatagggt atcatagcga tgagtagcgc
     1801 gcaaggccat ttaatagttc ccatgcttga cgatagtgtg ctatactaac aatgcaaact
     1861 agcaaatttc acagagaaag gatgctgaga ggtggcgaaa gagcagtctc ttgacaatct
     1921 ctggttggcg caggcattgt acgaacttgg gggcgttcaa ttcggaaatt tcaccgtcag
     1981 tgaatcggca gtcagttccc cggtatttgt gaaccccaaa gtccttatca gtaatccgac
     2041 cgccctgcgt gtagccagca agctgatgca gcaagaaatc aacctggcgc aatcgctgcg
     2101 ccgcccgcgc gttcatccct tcggctttgt agccggcgtt ccggtcggcg gcttgctgct
     2161 ggcaacagct tacgcgctgg aaaccaatat cccgctgatc tacgcccgca ctcgtcccga
     2221 aggcaccggc aaacgcggta tcgaaggacg ctacacacgc ggcgaaaccg tcctcgtcat
     2281 cgacgacctg attacgcgcg gcagcagcat cctcgaaacg gcggaattgc tggaggagag
     2341 tggtctgaaa gtcaaagatg tcatcgtgct gatcgaccgc gaacatggcg ccgttgaacg
     2401 tttgcgcagg cacaattaca atctcattag cattttgaag ctcgatgtga tgctcaccca
     2461 ttatttatca cg
//
LOCUS       DASZAK010000139         1548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_358436, whole genome shotgun sequence.
ACCESSION   DASZAK010000139 DASZAK010000000
VERSION     DASZAK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358436"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VGT44_02420"
     CDS             complement(<1..240)
                     /locus_tag="VGT44_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579682.1"
                     /translation="MHKQNTKRETVPTIWRVPDDLWARMAPILAELDPPKPTGRRRDP
                     RPRGRRCHHLPARVQGVNGISDPETSLTIVRSIARS"
     gene            complement(240..428)
                     /locus_tag="VGT44_02425"
     CDS             complement(240..428)
                     /locus_tag="VGT44_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579683.1"
                     /translation="MHAHTQDHALRVVAKLPEDEVPDGDAASPIKAVVIKEVHEGTRL
                     LSTRVTIMAWHGREWRCG"
     gene            complement(464..>1548)
                     /locus_tag="VGT44_02430"
     CDS             complement(464..>1548)
                     /locus_tag="VGT44_02430"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579684.1"
                     /translation="RALGIFGSVSGLGFTLGVILGGVLTSLFSWRWIFFVNVPLGVLV
                     LLGSLLLLPTSAARGQQKLDVAGTILGTSASAALVMTLSRLSELATSLTPFLVFLVLF
                     LMFTASFIFVEHASQHPLVPLSVFRIRNLLVANLLGALNLAIASIFGFTLTLYLQGVL
                     GLTALVTGLIFIPAGLGGIVGGRFAPQIIRRAGLHSALVTGPALIVLGSALMARITPV
                     DGALWVTLGYMLVGIGLGCGLVTLTIAVTNALGPEMQGLAAGLLNTSQQIGAALGASL
                     ASFVATSVVLALGNHGKAAVTTGDQVAIYLAGALALLAGALAVLAIRPSSTKPTSSEQ
                     HPSEIHPALALRLASFFVHLGEKNKA"
BASE COUNT          366 a          472 c          440 g          270 t
ORIGIN      
        1 ggaacgtgcg atggaccgaa ctatcgtcag ggaagtctcc gggtcactga ttccattgac
       61 accctggacg cgagccggaa gatgatggca tcggcgaccg cgcgggcgag gatctcgtct
      121 gcgccctgtg ggcttgggcg ggtctagttc ggccaggatc ggagccatcc gcgcccacag
      181 atcatcggga acccgccaaa tcgtcggcac tgtttctcgt ttcgtgtttt gcttgtgcat
      241 cagccgcacc tccactcgcg accatgccag gccatgatcg tcactctggt cgagagcaag
      301 cgggtaccct catgcacttc cttgatcacg actgctttga taggcgaagc cgcgtccccg
      361 tcaggcactt cgtcttctgg cagcttggcg accacacgca gcgcatgatc ctgcgtatga
      421 gcatgcatgc tttggtcagc ttctcgcttc ctgcaagaag gccttatgcc ttgttctttt
      481 cgccaaggtg tacgaaaaat gacgccaagc gtaaagccaa tgccgggtgg atctcagatg
      541 gatgctgttc gctcgaagtc ggcttggtag acgatggacg tatcgccagc acagctagcg
      601 caccggcgag caaagccaac gcaccggcaa gatagatcgc cacctgatct cctgtcgtca
      661 ccgctgcctt gccgtgattg ccaagcgcga gtactaccga ggtagcaacg aagcttgcca
      721 ggctggcgcc aagcgccgcg ccaatctgct gcgaggtatt caacagacct gcagccaacc
      781 cctgcatctc tggtccgaga gcattcgtca cggcaatggt cagtgtgacc aggccgcagc
      841 cgagaccaat tccgacaagc atatagccca acgttaccca gagcgctcca tccaccggtg
      901 taatacgagc catgagcgcg ctccccagga cgatgagagc tggccctgtc accagagcac
      961 tgtgcaggcc tgctcgtcga atgatctgag gggcgaaccg accgccaacg atcccgccaa
     1021 gacctgccgg gataaaaatc agtccggtca ccagcgcagt cagccccagg acaccttgca
     1081 ggtagagcgt cagggtgaac ccgaagatag aagcaatcgc aaggttcaat gctccgagca
     1141 gatttgccac caggagattg cggatgcgaa aaacgctcag cggcaccagt ggatgctggc
     1201 tcgcatgctc aacaaagatg aaggaggcgg tgaacatcag aaacaggacc aaaaagacca
     1261 ggaatggggt cagactggtg gcaagttctg agagccgcga aagggtcatc acgagtgcag
     1321 cactcgcgct cgtccccaaa attgttccgg ccacgtcgag tttctgctgt ccccgcgccg
     1381 cagatgtggg gagcaacagc aaagagccca gcagcaccag cactcccagc gggacattga
     1441 caaagaagat ccagcgccag gagaacaggc tggtgagcac cccacctaaa atgacgccaa
     1501 gcgtaaagcc aaggccggac accgatccga aaatccccag cgcacgat
//
LOCUS       DASZAK010000140         3697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_429508, whole genome shotgun sequence.
ACCESSION   DASZAK010000140 DASZAK010000000
VERSION     DASZAK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3697
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429508"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            509..886
                     /locus_tag="VGT44_02435"
     CDS             509..886
                     /locus_tag="VGT44_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579685.1"
                     /translation="MDRLQVFSARTREELNAQLEMANRDLAVRDARRVTASLSMQGAA
                     TGSASAEEETRASEGSQRNHSGNTLMLGASINVIEKTRIELESGKGGDHDTPYLCTLP
                     TTTPQMMAWIHLRNRVQNGELQA"
     gene            complement(968..2827)
                     /locus_tag="VGT44_02440"
     CDS             complement(968..2827)
                     /locus_tag="VGT44_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HEV2579686.1"
                     /translation="MQQTAPYLGEGPGPNSSHNPATASSILVADCGTTFTKVSLLGLV
                     EGQYRLLARGVAPTTAGPREDITRGIIQAINEIEYITGRRFVESGNILTPEDTSGNGV
                     DLFVSTISACETMRVVVLGAIDPELDMLTSKAVSGLNAETYVIPAPSYLEATTPNPLA
                     AGQGVPITQGVGSWKPEQVKAEWDRQLEQLRQLRPHAALIVGQAESPAGASPLQEACQ
                     LVVSAVQAIHKQDGKYQMPVIYAGAPQFVDAVRRMLNGYAELTRVETLTSPAQLGPTS
                     TAANALYERDAIQRIPGYDHLRAWSQTPSIGVASSLSSLVRFLAQHYAMNVTAIDVGS
                     SATTMMMAGELNDFIPLVNPGIGIGTGMGNILRQVGIERIVRWLPFTIAADEIQQYVT
                     NCVKRPQAIPSTHRELSIMQAFAREAIMLTYEAAMQDTSSPFDTDLILATGGVLSLVP
                     KYGQAALMLLDALQPRGVTSLVLDRTMLISQLGAVASVAPIAAVQVNENDAVMHRLGT
                     CVVPFGNLPAGQPAIRVGIEYSNGRQVTAEVMTGSIEIIPLKLNEQALLTLYPTPTVD
                     VGLGPGERARAAEEIDGGLVGLIIDARGRPLTFPGSDAERQARLLQWMQVMGA"
     gene            complement(2854..>3697)
                     /locus_tag="VGT44_02445"
     CDS             complement(2854..>3697)
                     /locus_tag="VGT44_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579687.1"
                     /translation="STWQGPKNVTPGQPDSGIAASSLLDANSLPQWMREDNQAAQGGV
                     SAGSGSPGLSAGSLIDMNNLPGWLRTAEQEPRSGYSSPGAPATFGGSGVYGTPGPTRI
                     ENMRVPSRPRAEMAPLEQSEMAANVFSSMLGVASASSAYPLATSSQMGDYPAQQSFQA
                     VQQAPGANQQWPTPAVSPFAQPGQQGAAPNAPAQGFAGAYQPAMQRVEYAAGYGVQNT
                     YGMPAGGVNSPVPSSPYAPPQPGYAPGGSGMGQASGEQRSETAGMKVARRGFLETIRG
                     WFHL"
BASE COUNT          784 a         1039 c         1077 g          797 t
ORIGIN      
        1 tcggctgagt aatttcactg ctgcctgcac agtcgcttca tcgctcattt caacagcttc
       61 attggtgtgt tttctctgtg caatcatgct aactcctttg tgatttatag taattgcctt
      121 atatgaggca atctccctgg cctcacacgc cattgatgtt atgtatcaag tctaacagcc
      181 ttgactgcat tctgtctatg aattgagata ggcgttatga atagtatcta taagtcgtga
      241 cttatagatg tggggaaatc tcctccgtgt acctcccttc cttacgaccg tactgcaaat
      301 aggtattttt cactatcaaa taatgacctg ttccagcgta caacgagtga tgggattgca
      361 agatgattgc acgataagga aagagaaaga gatgttttat cgagtagcta tcaggccaga
      421 tgttacagcg ccctggaagt ggagatcaac gccactgagt tcccgggatg ctctgtttgc
      481 gttcctccga ttgtaccaat caataccgat ggatcggctg caggtcttct ctgcgagaac
      541 tcgcgaggag ttgaacgcgc agcttgagat ggccaatcgc gaccttgcgg ttcgcgatgc
      601 ccggcgtgtt acggcatcgc tgtcgatgca gggagctgcc accggtagtg caagcgccga
      661 agaggaaacg cgggccagcg agggaagtca aaggaatcac agtggaaaca cactgatgct
      721 tggagcaagc atcaacgtca tagaaaagac tcgcatcgag ttagagtccg gcaagggtgg
      781 cgatcacgac acgccatacc tgtgcacact gccgaccact acgccgcaaa tgatggcctg
      841 gatacacctg cgaaacaggg tgcaaaacgg ggagttgcag gcataaacgc gcttagttat
      901 ccacatactt cgatggagcg tgtggataac taagcacagg tgtggaaaag gagctgttct
      961 tctatgacta tgcgcccatg acctgcatcc attgcaggag gcgggcttgc cgctcggcat
     1021 cgctgcctgg gaaggtcaag ggtctgccac gcgcatcgat aatcagaccg accagcccgc
     1081 catcgatctc ctcggcggcg cgggcgcgtt cgccagggcc gaggccgaca tcgacagtgg
     1141 gagtcggata gagggtcagg agcgcctgct cgttgagctt gagtgggatg atctcgatag
     1201 agccggtcat gacttcggcg gtcacctgcc gtccattgct atattcgatg ccgacgcgga
     1261 tagccggttg gcccgccggg agattgccaa agggaacaac gcaggttccc aggcgatgca
     1321 tgacggcatc attttcattg acctgcacgg cggcaatagg cgcgacgctg gcgaccgcac
     1381 cgagctgtga aatcagcatg gtgcgatcca gcacaagcga ggtaacacca cgtggctgga
     1441 gggcatccag cagcatcagg gcagcctgtc catatttagg gacgagtgag agtacgccgc
     1501 ccgttgccag gatcaggtca gtatcgaatg gactggaggt gtcttgcata gcggcttcgt
     1561 aggtgagcat gatggcttca cgcgcgaacg cctgcataat ggacaactcg cgatgtgtag
     1621 atggaatagc ctggggacgc ttgacacagt tcgtgacata ctgctgaatt tcgtcagcgg
     1681 caatggtgaa ggggagccag cgcactatcc gctcgatgcc cacctggcgc agaatattgc
     1741 ccatacctgt gccgatacca atgcccggat tgaccagtgg gataaaatcg ttgagttcgc
     1801 ctgccatcat catcgtggtt gcgcttgaac caacatcgat ggcagtcacg ttcatagcgt
     1861 aatgctgggc caggaagcgt accaggctgc tcagtgagct ggcgacgccg atcgagggcg
     1921 tttgtgacca tgcacgcaga tggtcgtagc cggggatgcg ctggatcgcg tcccgctcgt
     1981 acagggcatt ggcggctgtg ctggtcgggc caagctgggc gggtgaggtc agcgtttcga
     2041 cgcgagtcaa ctcggcatag ccattcaaca tacgccgcac tgcatccaca aactgcggcg
     2101 cgcccgcgta gataaccggc atctgatact ttccgtcctg tttgtggatg gcctgtaccg
     2161 ctgatacgac gagctggcag gcttcctgca ggggagatgc tccggcgggg ctttcagcct
     2221 ggccgacaat cagcgcagcg tgcggccgca gctgccgcaa ctgctcaagc tggcgatccc
     2281 attcggcttt tacctgctcc ggcttccacg agccgacacc ctgcgttatg ggtacaccct
     2341 gcccggcggc gagcggatta ggtgtagtgg cttccaggta ggatggcgca gggataacat
     2401 acgtttcagc attcaatccc gaaacagcct tggaggtgag catatcaagc tcgggatcga
     2461 tggcacccag caccacgacc cgcatggttt cgcaggcgct aatcgttgag acaaacaggt
     2521 caactccatt ccccgacgtg tcttccggtg taagaatatt cccactctcc acgaatcgac
     2581 gcccggtgat gtattcaatc tcgttgatgg cctggatgat gccccgcgta atatcttcgc
     2641 gaggaccagc cgtcgtcggc gcaacaccac gcgccaggag acgatattgg ccttcaacga
     2701 ggccgagtaa tgaaactttt gtaaaggtgg tgccacaatc cgcgaccagt atggaactgg
     2761 ccgtcgcagg attgtggctg ctgttcgggc ctggcccttc ccccaggtag ggtgctgtct
     2821 gctgcatctc cactatcctt gttgtatcta tcattagagg tggaaccagc cacgaatggt
     2881 ctcaagaaat ccgcgcctgg cgaccttcat gcctgccgtt tcacttctct gttcgccaga
     2941 cgcctgaccc ataccgctcc cgccaggcgc ataaccgggc tgtggaggtg catagggcga
     3001 cgaaggaaca gggctattaa cgccgcctgc gggcataccg taggtgttct ggacaccgta
     3061 ccctgctgca tactctaccc gctgcatggc cggttgataa gctcctgcaa agccttgtgc
     3121 tggcgcattc ggagctgctc cctgctgacc tggctgggcg aatggcgaaa cggcgggggt
     3181 tggccactgc tgatttgctc cgggcgcctg ctgcacagcc tgaaacgact gctgcgccgg
     3241 gtagtcgccc atctgcgaag aggtcgccag gggataggca gatgaagcag aagccacccc
     3301 tagcattgac gaaaagacgt tggccgccat ttcgctctgc tcgagaggcg ccatttctgc
     3361 gcgaggacgg ctgggtaccc gcatattctc aatgcgcgtc ggacctggtg tgccgtacac
     3421 tcccgaccca ccgaaagtag ctggtgcacc cggcgagcta taaccgctcc gtggctcctg
     3481 ctctgcggtg cgcagccagc ccggaagatt gttcatatcg atcagcgacc ctgctgataa
     3541 tccaggcgac ccactccccg cgctcactcc tccctgcgcc gcctggttgt cctcgcgcat
     3601 ccattgcggc agcgagtttg catccagcag cgaggaagcc gcaatacctg aatcgggctg
     3661 tcctggcgtt acgttctttg ggccttgcca cgttgaa
//
LOCUS       DASZAK010000141         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_429662, whole genome shotgun sequence.
ACCESSION   DASZAK010000141 DASZAK010000000
VERSION     DASZAK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429662"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VGT44_02450"
     CDS             complement(<1..1150)
                     /locus_tag="VGT44_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579688.1"
                     /translation="MTQLLGIPLDLLAKILVIITVIIIGSVVVLAIRNRIFFKIGVRN
                     IPRRRTQMVLIVFALMLSTTLLTGVLATGDVIEAGVQTVAVYNLGTVDETITGGHGAL
                     GTFDDYTYRRALDVERQDPNIAAVGAALVENNLLVADETSRQVRSSVTALALLPNTEK
                     GFGGMKDINTGKTDAISALGPNEVYLNQTMATLTNAHVGDTLYLYSQRWEGKRYQMQV
                     RGIVADGGLVGQTPYILSQYSTFLHIEGYRDALSEIYIALRNPNDPGGVDISDQVQQE
                     LRHAMPGDSNAHVSEVKEAGVKAAQTADDIFSRIFGLFALFALSIGLLLIFLIFVLLA
                     AERRAEMGMARAIGVQRRHLVLMYVFEGTVYDLISSFVGLVVGTSAGAG"
     gene            complement(1147..1950)
                     /locus_tag="VGT44_02455"
     CDS             complement(1147..1950)
                     /locus_tag="VGT44_02455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV2579689.1"
                     /translation="MASQLHASGPALPRLYSRTFAIFPARYKGYLLPALCLLLFFATG
                     CSAFSTSQAGQGNGSANTNTNTKITYVAIGASDAFGIGTDDPYNENWPTDLMNLLGAN
                     HIHLIDAGVPGILTHDALSLELPIALDAHPRLVTIWLGVNDIAANVPVSSYARDLQTL
                     VSRLRAAAPNASIAIANIPDLTILPYFSKSDPLQLTRIVQQYNAAIVSAVQQYHLILV
                     DLTQQNYNLKAHPEYISDDGLHPNSTGYQQLAKLFYEAIQAAQKQATTP"
BASE COUNT          433 a          566 c          602 g          417 t
ORIGIN      
        1 cccccgctcc cgccgacgtg ccaacgacca ggccgacgaa cgatgagatc aggtcgtaca
       61 cggttccctc gaagacgtac atcaaaacca ggtggcgtcg ctgcaccccg atggcgcgcg
      121 ccatgcccat ttcggcgcgc cgctcagccg ccagcagcac gaagatcagg aaaatcagca
      181 gcaggccaat ggagagcgcg aagagggcga acagcccgaa aatgcgcgag aaaatatcgt
      241 cggcggtttg cgctgccttg acgcccgcct ctttgacctc ggaaacatgc gcattcgagt
      301 cgcccggcat agcgtggcgt aactcttgct ggacttgatc cgaaatatcg actccgccgg
      361 gatcgttggg gttgcgcaga gcgatgtaga tctcggaaag ggcatctcta tagccctcaa
      421 tatgcaggaa ggtcgaatac tggctcagga tataaggcgt ctggcccacc aatccaccgt
      481 cggcgacgat gccgcgcacc tgcatctggt agcgtttgcc ttcccaacgc tgcgaataga
      541 gatagagcgt gtcgcccacg tgcgcgttcg tcagagtagc catcgtctga ttaaggtaga
      601 cctcatttgg accaagcgcg ctgatggcgt ccgttttgcc ggtattgatg tccttcattc
      661 ccccgaagcc cttttcggta ttggggagaa gcgccagggc ggtcacgctt gagcgtacct
      721 ggcgcgaggt ctcatcggct accagcagat tattctcgac cagcgccgcg ccaaccgccg
      781 cgatgttggg gtcctggcgc tcgacatcaa gcgcgcgacg ataggtataa tcgtcaaagg
      841 ttcccagcgc gccatgtcca ccggtaatcg tctcatcgac ggtgcccagg ttatagacgg
      901 caacggtctg cacacccgcc tcgatcacat cgccggttgc cagcacgccg gtcagcagcg
      961 tggtggatag catcagcgcg aaaacgatca gcaccatctg ggtgcgcctt cgtgggatgt
     1021 tgcgcacacc gatcttaaag aagatgcggt ttcttatagc cagaactacc acgctgccaa
     1081 tgataatcac agtgatgata accaggattt tcgccagcag atccagtggg atgccgagta
     1141 gttgtgtcat ggagtcgttg cctgtttctg cgcagcctgg atcgcctcgt aaaagagttt
     1201 cgccagttgc tgatagccgg tgctgttcgg gtgtagcccg tcatcgctga tgtattcagg
     1261 atgagctttc aagttgtagt tttgctgcgt cagatcgacc aggatcaggt gatattgctg
     1321 gacagcgctg acgatagccg cgttgtactg ctggacgatg cgggttaact gcagtgggtc
     1381 agacttgctg aaataaggca ggatggtcag gtctggaatg ttggcgatgg cgatgcttgc
     1441 attgggcgcg gcggctcgta agcggctcac cagcgtctgt agatcgcggg cgtagctact
     1501 cactggcacg ttggccgcga tgtcgttcac gcccagccag atcgtcacca gcctgggatg
     1561 cgcatcaagc gcgatgggca gttccaggct cagcgcgtcg tgggtgagga tacccggtac
     1621 gccggcatcg atcaggtgaa tatggtttgc gcccaacagg ttcatcaggt cggttggcca
     1681 gttctcgttg tagggatcat cggtaccaat gccgaacgca tccgatgcgc caatcgccac
     1741 ataggtgatc ttcgtgttcg tattcgtatt cgccgagcca ttcccctgac ccgcctgcga
     1801 cgtggagaac gccgagcagc cggtcgcgaa gaacagcagc aggcagagcg cgggcagcag
     1861 atagccctta tagcgcgccg gaaatatcgc gaaagttctg ctataaagac gcggcagggc
     1921 tggcccgctc gcgtgaagtt gcgacgccat ggtataagac caatctggca ggattgcacg
     1981 tcaaagcaaa aaacggttct cagatataat aacacatg
//
LOCUS       DASZAK010000142         1510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_3523, whole genome shotgun sequence.
ACCESSION   DASZAK010000142 DASZAK010000000
VERSION     DASZAK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3523"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="VGT44_02460"
     CDS             complement(<1..1418)
                     /locus_tag="VGT44_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579690.1"
                     /translation="MLRQLSRKPTPEPAPSEQPSAVSVSKGGAARIDTARNVGLIIKH
                     EYKKRVTQRSFIISTIILLVLIVLATFVPTVIKLIASRSNSQTKITIVNHVDTIAGLN
                     DGALTNYINTSLNGTNGATGQNTAGNAHFVIQMVSTDNTAGLQQQVKDGSLGILLVID
                     RAPNNKDLQYTYYSTSAPANDSDISQVQTLASQLSLLDKSTSLGLTPAQTTSLFAQPQ
                     FSDVNLGQAPSNRSEGDAVAGYILAYAGNILIYMAIILYGLGVAMGVAEEKGSRVMEI
                     LVNAATPFQLMAGKIIGLGAAGLTQMALLVIAGIGGLLLQIPLQAVLLGSNAGILNLN
                     ITGSTIALLLLLLLYFILGFLLYASLYAALGALVKRQDEVQNAVQPLTMVLVVGYLVG
                     FFGIYAPDATWMRVISYIPFWSPMVMLIRVGVSNVAWWEIVLTIVLMLGSIAICSIIA
                     GRIYRFGVLMYGQRPNLRQLVG"
BASE COUNT          316 a          423 c          443 g          328 t
ORIGIN      
        1 atcccgacca gttggcgcag gttgggacgc tggccgtaca tcagcacacc gaagcggtag
       61 atgcggcccg cgataatgga gcatatggcg atagagccga gcatcaacac gatggtgagt
      121 acgatttccc accaggcaac attgctgacc cccacgcgaa taagcatcac catcggcgac
      181 cagaagggga tatacgagat cacacgcatc cacgtcgcgt cgggggcata gatgccgaaa
      241 aagcccacca ggtatccgac gacaagcacc atggtcaggg gctgcacagc gttctgcacc
      301 tcgtcctgcc gcttcaccag cgcgcccagc gccgcgtaga gcgaagcgta gagcaagaag
      361 cccaggatga agtagagcaa gagcagcagc agcagtgcga tggtcgagcc ggtgatattc
      421 aggtttagaa tgccagcatt gctgccgagc agcacggcct gcagagggat ttggagcagc
      481 agcccgccga tgcctgcgat cactagcagc gccatttgtg tcagaccagc cgcgcccagg
      541 ccgatgattt tcccggccat cagttggaac ggcgttgcag cgttcaccag aatttccatg
      601 acccggctgc ctttctcctc cgccacaccc attgccacgc ccaggccgta gagaatgatc
      661 gccatgtaga tcagaatatt tccggcgtag gccaggatat acccggctac tgcatcgcct
      721 tccgagcggt tacttggcgc ctggcccagg ttcacgtccg agaactgtgg ctgcgcgaac
      781 aggctggtcg tttgcgcggg cgtcaggcca agactcgttg atttgtcgag caggctgagc
      841 tggctcgcca gcgtttgcac ctgcgatata tccgaatcgt tcgctggagc ggaggtcgaa
      901 tagtaggtgt attgtaaatc cttattgttt ggcgcgcggt cgattaccag caggatgccc
      961 agcgacccgt ccttcacctg ttgttgcaga ccagcagtgt tgtcggtgga taccatctgg
     1021 atcacaaaat gtgcgttccc cgccgtattt tgacctgtcg ctccgtttgt tccgttgagc
     1081 gaggtgttga tgtagttggt cagcgcgcca tcgttcagcc cggcaatggt atccacgtga
     1141 ttcacaatgg tgatcttcgt ttgcgagttc gagcgcgaag cgatcagctt tatgacggtt
     1201 ggcacaaacg tggcgagtac gatcagcacc agcaggataa ttgtgctgat aataaaactg
     1261 cgctgggtca cgcgcttctt atactcgtgc ttgataatca ggccgacgtt gcgcgctgta
     1321 tctatacgag cagcgccgcc cttgctgact gatacggcgc tcggctgctc tgaaggagct
     1381 ggttcagggg ttggtttcct cgaaagttgc ctcaacatgc ttgtgttcct tcctcctatg
     1441 ccggaatgct tgacgaggtt gatagcgctg atggagcgtc gggcaacgcg atcttgccga
     1501 cggcttcgat
//
LOCUS       DASZAK010000143         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_678060, whole genome shotgun sequence.
ACCESSION   DASZAK010000143 DASZAK010000000
VERSION     DASZAK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678060"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1229
                     /locus_tag="VGT44_02465"
     CDS             306..1229
                     /locus_tag="VGT44_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV2579691.1"
                     /translation="MATYTIEPERHTLHGHFSRELTPVLAIDPGDTVNYRTLDAGWGL
                     EPLLSFDSPRREFEPRDEELDTGHALCGPIEIRGAQPGMTLEVQINDIRPGAFGLTVA
                     DDLAIYKGLGIDRRALLTWTLDADTMTGYDQYGHTLTLRPFMGVMGMPPDEAGALSTS
                     PPRFCGGNLDCKELVAGSTLYLPIAVPGGLFSVGDGHALQGDGEVCGTAIECPMERVS
                     LTFRLRNDLRLTMPRANTPAGWLTMGLHKDLNQAMDMAMIGMLDLMEELYHLPRPEAL
                     ALASLVVDLRITQVVNGVRGVHALLPHGTLR"
     gene            complement(1235..>2554)
                     /locus_tag="VGT44_02470"
     CDS             complement(1235..>2554)
                     /locus_tag="VGT44_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2579692.1"
                     /translation="MMQETLSVSGILLTKTFGRQKYSQKRFEDENQKLTNLEVRQQMI
                     GRWFFMFIGTFFSVTPAVIYLVAGLQIIHNPLNPPITLGGIVAFTTLQSRIFFPIGQL
                     LNIQVEIQGALALFDRIFEYLDMPIEIQDAPNALSIQPEEMRGEITFNDVTFIYARDD
                     DFSPERLLAQVQPADDTAARVISPQPVELSAEALEATGEELEEQEPRPALSHVSFDIK
                     PGQLAALVGPSGAGKTTITYLVPRLYDVDYGSVELDGHDVREIALESLGKNIGVVTQE
                     TYLFHASVRENLLYARDDATEEQMIAAAKAAAIHDRIMELDNGYDTVVGERGYKLSGG
                     EKQRIAIARVILKDPRILILDEATSALDTHSERLIQAALEPLMKNRTTLAIAHRLSTI
                     LSADVILVVNRGEIVEQGTHDELMVLNGLYAQLYNEQFSREVSAALG"
BASE COUNT          545 a          741 c          749 g          519 t
ORIGIN      
        1 aaccagaaag ccacccacaa aattcccatt accaggagca ggacgcccag tagcgtttgt
       61 cccggctgtg aagaaaccat ccctaataca agcagcaaaa ccccaccaat gaggcaaact
      121 aacgcaataa attgatagaa ggtgttttct aaaggatcat cgtgcattcc ttttactcct
      181 gtctcataac tgctttcact tcaatacata ccagaaacgg gatacaatat ttacacgaga
      241 tacctttgga gagagataca gtcagagaga ctggctaccc attggaaatg gagcgataca
      301 gaagcatggc aacctacacc atcgagccag agcggcacac gctgcacggc catttctcac
      361 gtgaattaac accggttttg gctatcgacc ctggcgatac ggtaaattat cgtaccctcg
      421 acgcgggatg gggacttgag ccgctgcttt cattcgacag tcctcgccgc gagtttgagc
      481 ctcgcgatga ggaactggat accggccacg ctctctgcgg gccaatcgag attcgcggcg
      541 cgcagccagg catgacgctc gaagtgcaaa tcaacgatat tcgcccggga gcgtttgggc
      601 tgacggtggc agacgacctg gcgatataca agggacttgg catcgatcgt cgcgcgttat
      661 tgacctggac acttgacgcc gataccatga ccggatacga ccaatacggt catactctca
      721 ccctgcgccc cttcatgggc gtaatgggta tgccgccgga tgaggcaggc gcgctcagca
      781 ccagcccgcc gcgtttctgt ggaggcaacc tggactgcaa ggaacttgtg gcaggcagca
      841 cactctacct gcccatcgcc gtgccaggcg gcctgttctc cgtcggcgac ggacatgcct
      901 tgcagggcga cggcgaggtc tgcggcactg ctatcgaatg cccgatggag cgcgtcagcc
      961 tgaccttccg cctgcgcaac gacctgcggc tgacgatgcc gcgcgccaac acccccgccg
     1021 ggtggctcac tatgggcctg cataaggatc taaaccaggc aatggatatg gctatgatag
     1081 ggatgctcga cctgatggaa gaactgtatc accttcctcg cccggaagcg ttagccctgg
     1141 caagcctggt ggtggatttg cgcatcacac aggtcgtgaa cggtgtgcgc ggcgtccacg
     1201 cgctgctgcc gcacggaacg ctgagataat tgacttaccc cagcgctgcc gacacctcgc
     1261 gcgagaactg ctcgttgtac aactgcgcgt acaggccgtt gagtaccatc agctcgtcgt
     1321 gagtgccttg ctcgacaatt tcgccgcgat tgacgaccag gatcacgtcg gcggacagaa
     1381 tcgtcgaaag gcggtgcgcg atggccagcg tggtgcggtt cttcatcaac ggctccagcg
     1441 cggcctggat caggcgctcg gagtgcgtgt cgagcgcgct ggtggcttcg tccaggatca
     1501 gaatgcgcgg gtccttgagg atcacgcgcg cgatggcgat gcgctgtttc tcaccgccgg
     1561 agagcttgta accgcgctca ccgacaacgg tatcgtagcc attgtccagc tccatgatac
     1621 gatcatggat ggccgccgcc ttcgccgccg cgatcatctg ttcctcagta gcgtcatcgc
     1681 gcgcgtagag gaggttttcg cgcaccgagg cgtggaagag gtaggtttcc tgcgtcacca
     1741 cgccgatatt cttgcctagc gactccagcg cgatctcgcg cacatcatgc ccatcgagtt
     1801 cgaccgatcc gtaatcaacg tcgtacaggc gcggcaccag gtaagtgatg gtcgttttgc
     1861 ccgcgccgct cggtccgacc agcgcggcaa gttgccccgg cttgatgtcg aaggagacat
     1921 ggctgagggc cgggcgcggc tcctgttcct ccaactcttc cccggtcgcc tccaacgctt
     1981 cggcagaaag ctcgacaggt tgcggtgaaa tgacgcgggc tgccgtatca tcagcgggtt
     2041 gcacctgcgc gagcagtcgt tcaggtgaaa aatcgtcgtc gcgggcgtag atgaaggtca
     2101 catcgttaaa cgtgatttcg ccgcgcatct cctccggttg aatagatagc gcgttgggag
     2161 catcctggat ttcgatgggc atatccaggt attcaaagat gcggtcgaag agcgccagcg
     2221 cgccctggat ctcgacctgg atattgagca attgcccgat ggggaagaag atgcggcttt
     2281 gcagcgtggt gaaggccaca atgccgccga gcgtgatggg cgggttgagc ggattgtgga
     2341 tgatctgcag accggccaca aggtagatca cggcaggcgt caccgagaaa aatgtgccga
     2401 tgaacatgaa gaaccagcgt ccgatcatct gctggcgcac ttccaggttg gtcagcttct
     2461 ggttttcgtc ctcgaaacgc ttctgggagt atttctggcg gccaaaggtt ttcgtcagca
     2521 aaatgccgct gaccgagagc gtctcctgca tcat
//
LOCUS       DASZAK010000144         3609 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_110303, whole genome shotgun sequence.
ACCESSION   DASZAK010000144 DASZAK010000000
VERSION     DASZAK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110303"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VGT44_02475"
     CDS             <1..573
                     /locus_tag="VGT44_02475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2579693.1"
                     /translation="LLRQRGCRYTLAIYGSGPQRDELAELIDTFGLGDVVQLQGSCTQ
                     KELIALYREADLFVLSPHILENGDRDGIPNVLMEAMSVGLPVVATNVSGIPELIEHDR
                     SGLLVPPCDEGALAEALERLLDPLHGHALRARLGSGGRERVIEHFDAALHLRRMVELL
                     ETGGTRVAPVSPASMGPTRQEQLQVEGERM"
     gene            570..1850
                     /locus_tag="VGT44_02480"
     CDS             570..1850
                     /locus_tag="VGT44_02480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2579694.1"
                     /translation="MSPLRILYLCADPGIPVLGFKGASTHVRSLVSAFTETGHTVTVV
                     CTRRGEGNQLATELVEVAPDAQARQLGQLGDLAFARSGLSEKPGSELKRLLHNGPLGE
                     GVMNLCSTLQPDALYERYALLCNAGARVARTCNLPHIVELNAPLIEERQRYWGLALPD
                     VARTIEREVFSGADAILAVSAPVRDYAIAGGAAAQRVTVLPNAVDPLLFQALGNEVAA
                     AVRTRHDLGAAVVIGFVGSLKPWHGVDLLLEAFADLGDKQLRLLIVGDGPQAETLRLR
                     AAAPDLAGTVIFTGQVPHAQIGAYLAAMDIAVAPYRAPGPDQGSFYFSPLKVFEYMAA
                     GLPVVAPHLGQIVEVVKHGQHGLLYPPDDTGALTAHLLQLIRNPAQRTQMGRAGATQV
                     SQQYTWSGNAMRVAELLHQLRAERSPRCASKVMP"
     gene            1847..2503
                     /locus_tag="VGT44_02485"
     CDS             1847..2503
                     /locus_tag="VGT44_02485"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2579695.1"
                     /translation="MSESTSRTRLILVRHGATAWTESGRYQGSTADVPLSATGWSQVR
                     CLATALEPLRVASVVCSPLCRAVEMATCIAASHGLTATPERAFRELSFGDWEGLTSGE
                     AKARDPELYRRWKESPVTARPPGGETLQEVAERTIPALKAVIVRHAGEKVVLATHSLV
                     IRVLLCHALGAGLSTVAHLQCRPGSITVMDVQRDRYVVRLLNDTCHLQKDDLCRVVQG
                     "
     gene            2500..>3609
                     /locus_tag="VGT44_02490"
     CDS             2500..>3609
                     /locus_tag="VGT44_02490"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEV2579696.1"
                     /translation="MKSFKFRRGVLQVFWPYIRPARWKIGLALAILLLDTLADLASPW
                     PIKLIFDTVLLGKHLHQPWSLIIPQALAQNHPHLFIVLCATLLLLALISAGSTYVGMR
                     LLATTGQLVIFRLRSALFAHLQLLSPAFYDRQRLGDLLTRLTSDIQSIQDMLVTALPL
                     LLFNVMLVVGMLVVLLMINLSFGLLGLVSAFIVYLVLRRYLHTIKQVARQTRRSESDA
                     NAVVQENLRGIRVVQAVGLESHSQQKYEEHATRALHLGTIAAALQSGLPSVVGLLTDS
                     GNLAALTLGGILVMLGRVSIGDLLVFSAYLRTMYSPLRQLGKFSNLFTRASACAERVA
                     DLLHTAPAIVDRPTARAILRLQGALTFHRVSFRYDQ"
BASE COUNT          642 a         1079 c         1126 g          762 t
ORIGIN      
        1 ctactccgtc agcgcggttg tcgctacacg cttgcgatct atgggagcgg accgcaaagg
       61 gacgagctgg ccgagctgat tgacaccttt ggcctgggcg acgttgtgca attgcaaggt
      121 agctgcacac agaaggaatt gatcgctctc taccgcgagg cagatctgtt tgtgttgtca
      181 ccgcacatac tggagaacgg cgatcgcgat ggtatcccaa atgtgctgat ggaagctatg
      241 tccgttggcc tgcctgtagt ggccacgaac gtttcgggca tcccagaact gatcgaacat
      301 gatcggagcg gtctgctcgt gcccccctgc gatgagggtg cgctggcaga ggccctggag
      361 cggttgctcg atcccctgca tggtcatgcc cttcgcgccc gcctgggctc cggtggacgg
      421 gaacgagtga tcgagcactt cgatgctgct ttgcatctga gacggatggt cgaactgctg
      481 gagacggggg gcaccagagt agccccagta tcccccgcgt cgatggggcc aaccaggcag
      541 gagcagcttc aagtcgaagg agagcggatg tgagtccact gcgcatcctc tatctgtgtg
      601 ccgaccctgg tatcccggtc ctgggcttca agggggcgtc gacgcatgtg cggtccctgg
      661 tgagtgcctt caccgagact ggccacacgg ttaccgttgt ctgcaccagg cgtggcgagg
      721 gcaatcagtt agctacggag ctagtggagg tggctcctga tgcacaggcg cgtcaactgg
      781 gacagctcgg ggacctggct tttgcccggt cgggcctctc ggaaaagccc gggtcggaac
      841 tcaagcggct cctgcataat ggaccgcttg gggagggagt gatgaacctg tgctctaccc
      901 tgcagccaga tgctctctat gagcgctacg cactgctgtg caatgcggga gcacgggttg
      961 ctcgcacctg caacctgccg catatcgtag agctgaacgc accactcatt gaagagcgac
     1021 agcgctactg gggccttgcc ttgcccgacg ttgcacggac catcgagcgt gaggtcttct
     1081 ccggggcgga cgccatcctg gccgtctctg cgcctgtacg cgactatgcc attgctggag
     1141 gagcggcggc tcagcgtgtc acggtactgc ccaatgcagt tgatcccctg ctcttccaag
     1201 ccctaggaaa cgaggtggct gcagcggtgc gcacgaggca tgacctgggt gcagccgtag
     1261 tgatcgggtt cgtggggagt ctgaaaccct ggcatggggt cgatctgttg ttggaagcct
     1321 tcgccgatct cggtgacaag cagctacgcc tgctgatcgt cggcgatgga ccgcaggctg
     1381 agacgctgcg gctacgtgcg gcagcaccag atctggcggg aacagtgatc ttcactggtc
     1441 aggtgccgca cgcgcagata ggcgcttacc tggccgcgat ggacatcgcc gtggccccct
     1501 accgcgcacc aggcccggat caggggagtt tttacttttc tccactgaag gtattcgagt
     1561 atatggccgc cggactgcca gtggtggccc cgcatctggg ccaaatcgta gaggttgtca
     1621 agcacgggca gcatggctta ctgtacccgc ctgacgatac cggggcgctg acggcccatc
     1681 tgctgcagct gatccgcaat ccggcgcaga ggacacagat ggggcgtgca ggagccacgc
     1741 aggtttccca gcagtacacg tggtcaggga acgccatgcg cgtagctgag ctgctacacc
     1801 agctccgagc cgagcgatct ccccgctgtg cctcgaaggt gatgccatga gtgaaagcac
     1861 ttcacgcaca cgcctgatcc tggtgcggca cggtgcgacg gcgtggacgg aaagcggacg
     1921 ataccagggg agcaccgctg atgtaccgct ttcggccacg ggttggtcgc aggtcaggtg
     1981 ccttgccact gcccttgaac cactgcgcgt ggcaagcgtc gtgtgtagcc ccctgtgccg
     2041 ggcggtcgag atggcaacat gcatcgccgc ttcacatggg ctgaccgcta ctcctgaacg
     2101 agcctttcgt gagctcagct tcggtgattg ggaagggctg acaagcggcg aggctaaagc
     2161 acgcgatcct gaactgtacc gacgctggaa ggagagccct gtcacagcac gtccccctgg
     2221 cggtgagaca cttcaggaag tggctgagcg tacgatccct gcactcaagg ctgtcatcgt
     2281 acggcacgca ggcgagaagg tagtgctggc cacgcacagc ctggtcatcc gtgtgttgct
     2341 ctgtcatgcc ctgggtgccg ggctgtcgac agtggcccat ctgcagtgtc ggcctggctc
     2401 gataaccgtt atggatgtac agcgcgaccg gtacgtggtg cggctgctca atgacacgtg
     2461 tcacctgcag aaggacgacc tttgccgggt ggtgcaagga tgaaaagctt caagttccgc
     2521 agaggggtct tgcaagtctt ctggccctat atccgtcccg ctcgctggaa aatcggcctc
     2581 gctctggcga tcttgctact ggatacgctg gctgacctgg cctcgccctg gccaatcaag
     2641 ctcatctttg atactgtatt actgggcaag cacttgcatc agccctggtc gctgatcatc
     2701 ccccaggcgc tggcccagaa tcacccgcac ctctttatcg tgctctgcgc tacattgctc
     2761 ctcctcgctc tgatcagcgc tggctctacc tacgtgggca tgcgtctgct ggcaacaaca
     2821 gggcaactcg tcatcttccg tttgcgtagt gcactcttcg cccatctcca gctgctcagt
     2881 ccggctttct atgatcggca gcgcctgggt gacctcctga cgcgcctgac ctcggatatt
     2941 cagtccatcc aggacatgct ggtgacggcc ttgccactgc tgctgtttaa tgtgatgctt
     3001 gttgtcggca tgctggtcgt ccttctcatg atcaaccttt ctttcggtct gcttgggctg
     3061 gtgagcgcgt tcatcgttta ccttgtcctg cggcggtacc tgcacaccat caagcaggtg
     3121 gcacggcaga cccggcgcag cgagagcgac gccaatgcgg tggtccaaga aaacctgcgg
     3181 ggcatccgcg tggtgcaggc agttggcctg gagtctcact cccaacaaaa gtatgaggag
     3241 catgcgacga gagccttgca cctgggcacc attgccgccg ctctccagtc gggcctgcca
     3301 tctgtcgtgg gcctgctgac cgactcggga aacctggcgg ctttgacgct aggcggcatc
     3361 ctggtcatgc tgggacgtgt cagcattggg gatctgctgg tcttcagtgc ctatctgcgc
     3421 accatgtaca gccccttgcg ccagctcggt aaattcagca atctgtttac gcgggcgtct
     3481 gcctgtgccg agcgtgtggc ggatctgctc catactgctc cggcgattgt tgatcgcccc
     3541 acagcaagag cgatactccg cttgcaagga gctctcacgt tccatcgtgt gagctttcgc
     3601 tatgaccag
//
LOCUS       DASZAK010000145         1891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_429728, whole genome shotgun sequence.
ACCESSION   DASZAK010000145 DASZAK010000000
VERSION     DASZAK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429728"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VGT44_02495"
     CDS             complement(<1..708)
                     /locus_tag="VGT44_02495"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEV2579697.1"
                     /translation="MEYVRNLCTIGPGDIALVGGKAANLGVLLQKEMPVPPGYVLLTT
                     AYQKFLIANCIQAEIERLVASVNASKPTTVEQASIGIRDLFEQGNIPEEVSQAIRTAY
                     EQVGSSSVVIRSSATAEDLSSSSFAGQKESYLNVVGAEQVLAAVKRCWSSLWTARAIA
                     YRAQQGIPADGVSMAVIVQLMVPAKVSGVLFTINPVTGNQDEMVMNAAWGLGEALVSG
                     QVTPDTLLIEKASGQVKQ"
     gene            complement(762..1328)
                     /locus_tag="VGT44_02500"
     CDS             complement(762..1328)
                     /locus_tag="VGT44_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2579698.1"
                     /translation="MRRTREQILEALVRTIGQGVAGLSIPAVAREADVSIPTVYRYFH
                     TKQELVSALGGYLMQKIGLSDLQPPRSLDELLEITRQSFIKSEGLDDAIKAATVSELG
                     NQFRKGAMPVRIKFLEQAIAAELAPLNERDRVRLRNMVLILTSSAMIRAFQEYLDLSG
                     EEAANNVIWAVRLLVRAATQQAEAASDG"
     gene            1481..1563
                     /locus_tag="VGT44_02505"
     tRNA            1481..1563
                     /locus_tag="VGT44_02505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1514..1516,aa:Leu,seq:caa)
BASE COUNT          421 a          564 c          446 g          460 t
ORIGIN      
        1 ctgcttcacc tggccactgg ctttctcaat caaaagcgta tcgggggtca cttgaccgct
       61 gacaagcgct tctcccaatc cccacgcggc gttcataacc atttcatcct ggttgccagt
      121 gacagggttg atcgtaaaaa gcacgccaga tacttttgca ggcaccatta gctgcacaat
      181 caccgccatg ctcacaccat ccgccggaat gccctgctgc gcgcgatatg caatggcgcg
      241 tgcggtccac aacgacgacc agcagcgctt cactgccgcc agcacttgct ccgcccctac
      301 gacattcaga tagctctcct tctggccagc aaaagaactg ctcgacaggt cttccgcagt
      361 tgcggatgaa cgtatcacaa cagaactact ccccacctgt tcataggcag tgcgtatcgc
      421 ctgggagacc tcttccggta tattcccctg ctcgaacagg tcgcgaatac cgatagatgc
      481 ctgctcaact gtcgtgggct tgctcgcatt cacagatgcg accagtcgct cgatctccgc
      541 ttgtatacaa ttggctataa gaaatttttg gtaggcggtt gtgagcagca cataaccggg
      601 tggaaccggc atttccttct gtaacagcac gccgaggttg gctgcttttc cgccaactaa
      661 cgcgatatcg cccggtccga ttgtacacaa attacgcaca tattccatag ccagacctac
      721 cttcctgcat cgctcctgcc ctggtaggac tcgatggagc gttacccatc cgaggcggct
      781 tctgcttgct gcgtggcagc gcgaacgagc aggcgaaccg cccagatgac attattcgca
      841 gcctcttcac cggacaggtc gagatattct tgaaaggcac ggatcatcgc cgatgaggtg
      901 aggatcagca ccatattgcg tagtcggacg cgatctcgtt cattcagagg tgccagttcg
      961 gcagcgatgg cctgctcgag aaacttgatg cgcacgggca tcgccccttt gcgaaactgg
     1021 ttgcccagtt cgctgacggt cgctgccttg attgcgtcgt ccagcccctc ggatttgata
     1081 aagctctggc gcgtgatttc cagcaactca tccagcgagc gcggcggttg gagatcgctc
     1141 aggcctatct tctgcatcag atagcctccc aacgctgaaa ccagttcctg tttcgtgtga
     1201 aaataccggt aaacggtcgg aatggacacg tcggcttcac gcgcaactgc cggaatagac
     1261 aatccagcaa cgccctggcc aatcgttctg acgagcgctt caagaatttg ctcacgcgtt
     1321 ctccgcacct gctcttgtcg gaggctgcta cgatattctt tattcccttt cataagatga
     1381 atcgtatcac aaatatatga atcatatcaa gatcgagcga aagggatgat aaagcagcta
     1441 ttgacgggtt tcggctggtg tggtactatc tatcacacca gccggcgtgg cggaacggta
     1501 tacgcgcttg tctcaaaaac aagtccccgt gaggggttga gggttcgaat ctctccaccg
     1561 gcatagccca tccataggtg tcggtggttg tcgattccac gctcactttc ccgcttttgt
     1621 ggcacatctg caactcccgt gctcaacagt acttctcttt atccacttgc cacaatgaca
     1681 attgtcgagc ataggagttg ttaatgatcc acaaaagtga gtcaacttca cttagtgcca
     1741 ttacgctaac acataccgtc gcgcggctct ctgaccacta ccttttaacc ttccccgttc
     1801 taccaacttt tccaaatccc tgtgagccgt cctatcagtc accccggtca attttctata
     1861 aatcccattc gtgatagacc catgttcctg a
//
LOCUS       DASZAK010000146         1763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_216643, whole genome shotgun sequence.
ACCESSION   DASZAK010000146 DASZAK010000000
VERSION     DASZAK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216643"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VGT44_02510"
     CDS             complement(<1..785)
                     /locus_tag="VGT44_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579699.1"
                     /translation="MATNTVETPETNAAEQQQGAVAVSSGAAAQNNLRNIGLILGREY
                     KNRVTQRSFIITSVILLVIVFLAAFIPTVIQLVSSGSSSSGQTSIVVVNEAGSVAGLN
                     EAELTADITTALNGTSTGSSAPYVLSIQPQTSLSSLQSQVKDGKLDMLLVLERPTNQN
                     VRFTYYTTNSSTNDPNLSAIQGLTLQLTFFDTAHRLGLTSSQTQRLAAAPDLVVVQTK
                     PSQTTQLTSESAAGFVLATAGVILIFISIQVYAGIVATGVAEE"
     gene            complement(802..>1763)
                     /locus_tag="VGT44_02515"
     CDS             complement(802..>1763)
                     /locus_tag="VGT44_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2579700.1"
                     /translation="CFVKGLFLMGLKVTGVSKSFGTFQAIKDLSMEVREGALFGFLGA
                     NGAGKTTTMRMILDLFRPDSGQITWNGEDVRKVPRRSFGYLPEERGLYPKMRVDEQLL
                     FLARLNGLSKQDAEKELTEWLDRFQISANRKKKIEELSKGNQQKVQFLATILHDPTIL
                     VMDEPFSGLDPVNANVLKEAFLEMHRRGKTIIFSTHQLEQAEELCQEIVIINKGQSVV
                     QGSVREVKRKPGRQVARLKLDNDAQATWLEQLAGVQVTRRREDYIEMQLPAHLNPNVI
                     VEAALQHGGIISRFEIAEPSLTDIFIELVGMSALPDVPPALSA"
BASE COUNT          352 a          505 c          508 g          398 t
ORIGIN      
        1 ttctcttccg ccactcccgt cgccacgatc ccggcataga cctggataga gatgaagatc
       61 aggatcaccc cggcagtagc gagcacaaag ccggcagcac tttcgctcgt cagctgagtc
      121 gtctggctag gctttgtctg cacgacgacc aggtcaggtg cagcggccag gcgctgagtc
      181 tgagacgagg tgaggcccag gcgatgagcg gtatcgaaaa aggtgagctg caaggtcaaa
      241 ccctggatcg cggacaggtt gggatcgttg gtcgaactgt tggtggtgta ataggtaaag
      301 cgtacgttct ggttcgtcgg acgctcaagc accagcagca tatccagctt gccatccttg
      361 acctggcttt gcaggctgct tagagaagtc tgtggctgga tgctcaagac atagggagcg
      421 gagcttccag tgcttgttcc attcaatgca gtagtgatgt cagcggtcaa ctccgcctcg
      481 ttcagaccgg cgaccgagcc agcctcgttc accacgacga tactcgtttg cccactggaa
      541 ctggaaccgg agctcacaag ctgtatcacc gtcgggataa aagcggccag gaacacgatc
      601 accagcagga tcacactggt gataatgaaa ctgcgctggg tgacccggtt tttgtactcg
      661 cgcccgagaa tcaagccaat attgcgcagg ttgttctggg cagcagcgcc cgaactcacc
      721 gcgacggccc cttgctgttg ttccgccgcg ttcgtttctg gcgtttccac ggtgttcgtt
      781 gccatggttt ctttctcctg tctatgctga gagagccggt ggaacgtctg ggagggcact
      841 catgcctacc agttcaatga agatgtcggt cagggatggc tcggcgattt cgaaacggct
      901 gatgatgccg ccgtgctgca gcgcggcttc cacaatcacg ttggggttga ggtgagccgg
      961 gagttgcatt tcgatgtaat cctcccggcg cctggtgacc tgcacgcccg ccagctgctc
     1021 cagccaggta gcctgggcat cgttgtcgag tttgaggcgg gccacctggc gtccaggctt
     1081 tcgtttgacc tcgcgcacgc tgccctgcac gacgctctgc cctttgttga taatcacgat
     1141 ctcctggcac agttcctcgg cttgctccaa ctggtgcgtg gagaagatga tggtcttgcc
     1201 ccggcgatgc atctccagaa atgcctcttt cagcacgttg gcgttgaccg ggtccaggcc
     1261 gctaaacggc tcatccatca ccaggatggt cgggtcatgc aggatggttg ccagaaactg
     1321 taccttctgc tgattgcctt tggagagttc ctcgatcttc ttcttgcgat tggcgctgat
     1381 ctgaaagcgg tcgagccact cagtcagctc tttctcggca tcctgcttcg acaagccgtt
     1441 caagcgtgcc agaaacagca gttgctcatc cacgcgcatt ttgggataga gtccgcgctc
     1501 ctccggcaga tagccgaaag agcggcgcgg caccttgcgc acatcttcgc cgttccaggt
     1561 aatctggccg gaatcgggac ggaagaggtc gaggatcatg cgcatcgtcg tcgtcttccc
     1621 ggcgccattc gcgcccagga agccaaagag cgcgccctcc ctgacctcca tagaaagatc
     1681 tttgatggcc tgaaatgttc caaaagattt ggaaacgcct gtcaccttga gtcccatgag
     1741 aaaaagtcct ttcacgaaac aaa
//
LOCUS       DASZAK010000147         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_74930, whole genome shotgun sequence.
ACCESSION   DASZAK010000147 DASZAK010000000
VERSION     DASZAK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74930"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..687
                     /locus_tag="VGT44_02520"
     CDS             <1..687
                     /locus_tag="VGT44_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2579701.1"
                     /translation="NSDGSAQVWTATTGRTIMSFHEHQGEILALAWSPVSDSLIASGG
                     KDKTIRIWDAGNGTHFNPYTGHTGSVRAVAWSRQGILASAGEDKTVRTWGYVNERKDY
                     MYHGHTAAINALAWSPDGAYLASASDDGTVRVWDTVNNSLAYTYRRHTGPVNAVTWLS
                     RFGGIQMRLASGGDDATVQVWNVAGEHIETYGGLTSRVRSLTSAKFGSDQRIAFGSDD
                     GTVHVWTPGQ"
     gene            761..1993
                     /locus_tag="VGT44_02525"
     CDS             761..1993
                     /locus_tag="VGT44_02525"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV2579702.1"
                     /translation="MTEQTDLELDNFFATIQNPYPVYAYLREYEPVRFNPMFQTYMLS
                     RYEDVNMAFSDHRRFSSDIWSGVAETLGFAGEDEASKNLGKIIPFLQYNLQGMDPPGH
                     TRQRTLMTKTFTPRMIESFRPTVQKLVDELIDQRLDEGSMDLVADFAYPLPSNVILDL
                     LSIPRSGRPYIKASSEAINEFIATLIFNGPAVWPRLAGVFDDMTAYLKSLIAERRAHP
                     GADLLTKMVQAEEHGDMLSEDEIVISTNFLLFAGHETTANLIAVGMYHLMQNPDQLAQ
                     LRADPSKTPAAVEELLRYVSPVHTLGRRTVEEVTLHGVTIPPESGIYLLVGAANRDAE
                     KFPDPEKLDINRPPARTLGFGYGIHFCVGAALARLESQVAFETLLRRLPGIHMTGETP
                     LFRPNYSLRGLVALPVEF"
BASE COUNT          424 a          608 c          587 g          394 t
ORIGIN      
        1 aacagcgatg gctcggccca ggtatggacg gcgaccaccg gcaggacgat catgagtttc
       61 catgaacacc agggggaaat tctcgcgctg gcctggtcgc cggtgtccga ttcactgatc
      121 gcttccgggg gcaaggataa gacgattcgc atctgggatg ccggtaatgg cacccacttt
      181 aacccgtata ccggccatac tggaagtgtc agagcggttg cgtggtcgcg gcaaggcatt
      241 ctcgcgtcag ccggcgagga taagacggtg cgcacctggg gctatgtcaa tgaacgaaaa
      301 gactacatgt atcatggaca cacagcggct atcaacgccc tggcctggtc gcccgatggc
      361 gcatatctcg cctccgccag cgatgatggc acggtgcgcg tctgggacac cgtgaataac
      421 agcctggcct acacctatcg caggcatacc ggcccggtga acgcggtcac gtggctgagc
      481 agattcggtg gaatccagat gcgccttgcc tccggcgggg acgacgcaac ggtgcaggtc
      541 tggaatgtgg cgggagagca tatagaaacc tatggcgggc ttacatctcg tgttcgatcg
      601 ctcacttctg cgaaatttgg ctccgaccag cgcattgctt ttgggagcga tgatggcacc
      661 gttcacgtct ggacacctgg gcaatgacaa atcttgcgct atcaagatac agcgtatata
      721 ctttttgggg agttacacag gaacgcatgg agcaattacg atgaccgagc agacagacct
      781 ggaactggac aattttttcg cgacgatcca gaacccctat ccggtgtacg cctatctgag
      841 agaatacgag ccggtgcgct tcaaccctat gtttcagacc tatatgctca gccgctacga
      901 ggatgtgaac atggctttct ccgaccatcg ccgtttctcc tcggatatct ggtcgggtgt
      961 ggccgagacg ctcggtttcg cgggcgagga tgaggcgagc aagaatctgg gtaaaatcat
     1021 cccgtttctg caatacaacc tgcaaggcat ggacccgccc ggccacacgc gccagcgcac
     1081 gctgatgacc aagaccttca cgccgcgcat gatcgagtcg ttcagaccga ccgtgcaaaa
     1141 gctggtggac gagctgatcg accagcgact ggatgaaggc agcatggacc tcgtggcaga
     1201 cttcgcctac ccgctgccct cgaacgttat tctggacctg ctgagcattc cgcgcagcgg
     1261 gcggccctac atcaaggcca gttcagaagc catcaacgaa tttattgcca cgctcatctt
     1321 caacggcccg gcggtctggc cccggctggc gggcgtcttc gacgatatga cggcgtatct
     1381 gaagagcctc atcgcggaac ggcgcgcgca tcctggcgct gatttgctga cgaagatggt
     1441 gcaggccgaa gagcatggcg atatgctgag cgaggatgaa attgtgattt ccacgaattt
     1501 tctgcttttc gccggtcatg aaactaccgc caacctgatc gccgtcggca tgtatcacct
     1561 catgcaaaac cccgatcaac tggcgcagct gcgcgcagac ccgtccaaaa cgcccgccgc
     1621 ggtcgaggag ctgctgcgct atgtcagccc ggtacatacg cttggccgcc gcaccgttga
     1681 agaggtcacg ctgcacggcg tcaccatccc gcctgagagc ggcatctacc tgctggtcgg
     1741 cgcggccaac cgcgacgccg aaaagttccc cgatcccgaa aagctggata tcaaccgccc
     1801 accggcgcgc acgctaggtt tcggctacgg catccacttt tgcgtcggcg ctgccctggc
     1861 ccgcctggag agccaggtcg ccttcgagac cctgctgcgg cgtctgcccg gcatacacat
     1921 gaccggtgaa acgcccctgt tccgcccgaa ctactccctg cgcgggctgg ttgcgctgcc
     1981 ggtggagttc tagcaattca agcaaattga cca
//
LOCUS       DASZAK010000148         7529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_286983, whole genome shotgun sequence.
ACCESSION   DASZAK010000148 DASZAK010000000
VERSION     DASZAK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7529
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286983"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="VGT44_02530"
     CDS             complement(<1..752)
                     /locus_tag="VGT44_02530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEV2579703.1"
                     /translation="MKKKETQSSFSIVSDGAQGFVLSTVLDLSKLENHLWEAANILRG
                     PVDAADFKTYIFPLLFFKRISDVYDEEREAALTESGGDQKFASFPEIHRFQIPGGCHW
                     RDVRARTTNVGQALQMAMREIEKANQRTLYAIFGDAQWTNKDRLSDALLVDLLDHFSR
                     LPLSNGDVDSDVLGQAYEYLIKKFADATNKKAGEFYTPRSIVRLMIRILDPHEGETIY
                     DPACGTGGMLLEAVHHVRETGGNARTLWGKLYG"
     gene            complement(881..1267)
                     /locus_tag="VGT44_02535"
     CDS             complement(881..1267)
                     /locus_tag="VGT44_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2579704.1"
                     /translation="MNGSIKDILERPRFECPVDGFQQMINGKYKLRILWALKLGPRRY
                     GEIKKGLLRDISGSQEIAPRVLSRELKTLAAYGLIERTDYQVVPPKVEYALTPLGRSL
                     LPLMAQIAEWGRQNLVRDAALAANEA"
     gene            1622..2503
                     /locus_tag="VGT44_02540"
     CDS             1622..2503
                     /locus_tag="VGT44_02540"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2579705.1"
                     /translation="MILVTGATGLNGSELVRRLSARGIPVRALVRNTARAGALSSLPM
                     VEIVEGDMARPETLTGALRGIDRAMLISSSDPAMLEVQSNFIEAARKAGVKHVVKLSG
                     IMPELDSPFRFARMHGEIERRLEDSGMAFTHLRAGEFMHAYFRQVPSIVARGTMFLPM
                     EDARIASIDIADVAEVAATVLTGPGHEGKIYPITGPEALSMAEVAEKLSNATGKRIQY
                     VNVPPEEAKRAQLADGVPPFTADALAELFAERRKGKEAQVSPIIQTIFGWRATSFDEF
                     ARRNAAIFRGEQPAPKV"
     gene            complement(2805..4985)
                     /locus_tag="VGT44_02545"
     CDS             complement(2805..4985)
                     /locus_tag="VGT44_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2579706.1"
                     /translation="MSDPLPLDSNELTPDDLDVIRDFLATEDFATTSDDDSAAMSEIA
                     AVYGWTGALDEAGEEEEMLSIFVTEVDEDIADMQSALALLRQDQTLAILPALTPMLPS
                     AISALSALRRCAHKIRGTSAAIGCKAMSTIAHSVETMIEQANGQQIEWQSGMMALGFA
                     IDALRETLESVATHGQESILPLLTLEERYADLHLPLDSTGRSEGSAGDEPDEVGGIGA
                     WRDARRGDMMEDTLHAPEWAEYLAKLLSHVEQLAERQHEIEDARREVDTALAELRASQ
                     ARLRRIETLVAALPISSPATAEANGSRQASIDELAPSSLVARILRESAQRTGHDYQPR
                     QQSYATQSPFASSASLAESMLWDELELDHFTETHMLSRSLNEAIADVATTTAQLQLAL
                     LHLDALNRQQASMTLLIRDDARSLQIAPELDTPYEARGATPGLLVRVGDQHAIVPFSQ
                     TLRIDYSGYTGQVYALGDLLGLPPLVGEKTERVLPLLLVRPDLLETPGVTPDEQRTGE
                     PVAVQVDEALGQVELRVKPLPLALRRPGITGAVITRTGEVALAIDLPTLIRRHKQREQ
                     GLSDLVEGEEATSASVLAMSMEGQDAQLARSQAEKVARVLIVDDSVYMRRSLAKTLAS
                     KGYKLFEARDGVEALEQLMGTSNASPDVLLLDLEMPNLNGYELLNILRTRALLPDLKI
                     ILLTSRASDKHQKRARDLGIHAYLTKPCSEETLLETVAGVLGRR"
     gene            complement(4978..5487)
                     /locus_tag="VGT44_02550"
     CDS             complement(4978..5487)
                     /locus_tag="VGT44_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEV2579707.1"
                     /translation="MLPFDALPAQRLWDMSDEEFWAYARELAGGGSSTPVRADAGQYL
                     EIMLSRGMCLTQLSSIEEVLPSPSHYTLLPSVPGWMLGLAAWRGEVMAVVDLEMYLVG
                     HTDSRIGSGGLLLCLHYGELALGLFVPAVGETVAPGGEPQQTGAAALDFEALLADIAR
                     QIGMAAHNV"
     gene            complement(5500..5892)
                     /locus_tag="VGT44_02555"
     CDS             complement(5500..5892)
                     /locus_tag="VGT44_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2579708.1"
                     /translation="MMSKLVMVIDDSLTVRKIIETSLRREGIPSISYADGVEALRALK
                     ERRCPVPDLVILDIGLPKMDGYEVARFFKTKEQFNNTVIVMLSKRDGVIDRLKGRLAG
                     AKEYITKPFRTQEVISVVHTHLGAPSFS"
     gene            complement(6286..7374)
                     /locus_tag="VGT44_02560"
     CDS             complement(6286..7374)
                     /locus_tag="VGT44_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2579709.1"
                     /translation="MSHAQIDYEHFFRQLLDALLLPLVIVDASLVIRFSNDKARTLFG
                     MSEPVEGMLLERVIHDEGVLQMIRRSIEARQPAREERLTGEAERMSGSAWLVSVRPLA
                     HSKTARTLRYFAVVIEDLTELRRLERARRDFVANISHELRTPLASVRLLVETLEEAID
                     TDPEQAQVFVGKIENEIEHLSMLVAELLDLSRIESGQTPMVIEPVEAEQLVREVMARM
                     LPLAQRHRVSLRTEIEQGQTLVAADVKQVARVLVNLAHNAIKFTPSGGAVVIGTCAQS
                     SNLQAFYVRDTGVGIAPEDLPRIFERFYKVNRARSKADFIGPGGAGTGLGLAIARHVV
                     EAHGGRIKAESVPGQGSVFTFTLPNTTR"
BASE COUNT         1630 a         2300 c         2019 g         1580 t
ORIGIN      
        1 ccatacagtt tgccccatag cgtcctggca ttcccacccg tctcgcgcac gtgatgcacc
       61 gcttcaagca gcataccgcc tgttccgcag gcgggatcat agatcgtttc gccctcgtgc
      121 ggatcaagaa tgcgaatcat caggcgcaca atgctgcgcg gcgtatagaa ctcgcccgcc
      181 ttcttgttag tggcatcagc gaatttcttg atgaggtatt cgtatgcctg tcccagcaca
      241 tcggagtcta catcgccatt cgataacggc aggcgcgaaa agtgatcgag caggtcaacc
      301 agcagggcat ccgagaggcg atctttatta gtccattgcg cgtcgccaaa aatggcatag
      361 agcgtgcgct ggttggcctt ctcaatttcg cgcatggcca tttgcaacgc ctgcccaacg
      421 ttggttgtcc gcgctcgcac atcacgccag tggcagccac cgggaatctg gaagcgatga
      481 atctccggga aggatgcaaa tttctggtct ccgcccgatt ccgtcagcgc cgcttctctt
      541 tcttcatcat aaacatccga gatacgcttg aaaaagagca aaggaaaaat gtaggtcttg
      601 aaatccgccg cgtccactgg tccacgcagg atattcgccg cttcccacag gtgattttcc
      661 agtttcgaga ggtcgaggac tgtactcaac acgaacccct gcgccccatc agagacaatg
      721 gaaaagctgc tctgcgtctc tttcttcttc atctcaatac atatgctcta gaatacccac
      781 aatatatgca atggctgtgc cttcccgatt gtactatact tcacgcgaga atcatagagc
      841 atatcctaat caggtcctat aacccacagt ctgaggcccg ttacgcctcg ttcgccgcaa
      901 gagcagcatc acggaccagg ttctgcctgc cccactccgc gatctgtgcc atgaggggca
      961 gcagactgcg acccagaggc gtcaaggcat actccacttt tggaggcaca acctggtagt
     1021 cggtccgctc gatcagacca taggccgcca gtgtcttcaa ttcgcgactg agtacgcggg
     1081 gcgcaatttc ctggctgccg ctgatgtctc gtaaaagtcc cttcttaatc tccccatacc
     1141 gccggggtcc gagcttgagc gcccagagaa tccggagttt gtattttcca ttgatcatct
     1201 gctgaaaccc gtcaacggga cactcaaatc gcggtcgctc taggatatcc tttattgagc
     1261 cgttcatctc atttctccaa tctgcaccct ggtggtacgg agcaatggtc tgtttgattg
     1321 tttcctggtc tatagtatca tttttgtccc tacttgtcag gatgtgcata ggcgcgtatt
     1381 atacccctaa ggcagcagac atgtacagag acttgtcaaa tgtgtgattc aggcaacaaa
     1441 aggtcaacta tgatgaactt gaacgaccaa agccggtcct gtcatgctga gcgcagcgaa
     1501 gcatctcgct cgttgggccg agagatgctt cgctgcgctc agcatgacag gaccgatgat
     1561 ggttgttaaa aatcatgact actcaaaaac gcaagcagca gttgtcataa ggaggcagaa
     1621 gatgattctt gtaaccggtg ccaccggact gaacggaagt gagttggtac gccgactatc
     1681 ggcaaggggc attcctgtgc gcgccctggt gcgcaacaca gccagggccg gggcgttatc
     1741 ctctctgccg atggtcgaga tcgtagaggg tgacatggcg cgccctgaga cgcttaccgg
     1801 agcattgcga ggaatagacc gcgccatgct catctcgtcc tcagaccccg cgatgctcga
     1861 ggtgcaatcg aacttcatcg aagcggcccg taaagcgggc gtcaaacacg tcgtcaaact
     1921 ttccggaatc atgccagaac tcgactctcc ctttcgcttc gcccgtatgc acggcgagat
     1981 tgagcgcagg ctggaggact cggggatggc attcacccac ctgcgcgccg gtgagttcat
     2041 gcacgcctac tttcgacagg tgccatcgat cgtcgccagg gggaccatgt tcctgcctat
     2101 ggaggatgcg aggatcgcgt cgatcgacat cgccgatgtc gccgaggttg ctgccaccgt
     2161 cctgactggc cctggacacg aggggaaaat ctacccaatc accggtcccg aggcactcag
     2221 catggcagag gttgcagaga agctctccaa cgccaccgga aagaggattc agtacgtcaa
     2281 cgtccctccc gaggaagcaa agagagcgca gcttgccgac ggagtgccac catttacagc
     2341 cgatgctctg gccgaactct ttgccgagcg acgcaagggc aaggaggcgc aggtttcacc
     2401 gatcatccag acgattttcg ggtggcgagc aaccagcttc gatgagtttg ccaggcgcaa
     2461 tgccgcgatc ttccgtggcg agcagccggc gccgaaggtc tgatgcctcg cccaaacgca
     2521 tacatctgca acatccgata caactgatac attagataca ttggatacat tgcccatcac
     2581 caacacacga cacaactgag ccactctccg cctgcgagga cgcgataaag aactttcaca
     2641 aacaagatcg gcaaccgagc ctgtcatcct tcgctgcgct caggatctca ctcgccggac
     2701 accgagatcc ttcgctgcgc tcaggatgac attaccggtg gtggtcgtga aacttcataa
     2761 tcgcgtccta cgacgggaaa tgacatttac ggagcatata ccctttacct gcgtcccaac
     2821 actcccgcca ccgtctccag tagcgtctcc tctgagcagg gtttggtcaa gtaggcgtgg
     2881 atgccgagat ctcgcgcgcg cttctggtgt ttatcagatg cgcgcgaggt cagcaggatg
     2941 atcttgaggt caggcagcag ggcgcgggtg cgcagaatat tgagcagctc atagccgttg
     3001 aggttaggca tctccaggtc gagcagcagc acatcgggcg aggcgttcga tgtccccatc
     3061 aactgctcca gcgcctcgac gccgtcgcga gcctcaaaca gcttgtagcc cttcgaggcg
     3121 agcgttttag ccagcgagcg gcgcatatac accgaatcat ctacgatcag cacacgcgcc
     3181 actttctcgg cctggcttcg cgccagctgc gcatcctgtc cctccatact catcgccaga
     3241 acgcttgcac tcgtggcctc ttcgccctca accagatccg acaagccttg ctcgcgctgc
     3301 ttgtggcggc gaatcaacgt gggcagatca atcgccagcg ccacctcgcc ggtgcgcgtg
     3361 atgaccgcgc cggtgatgcc gggacgccgc aaggccaacg gaagcggctt gacgcgcaat
     3421 tcaacctggc ccaacgcctc gtcaacctgc acggcgacag gttcgcctgt gcgctgttca
     3481 tccggggtca ctccaggcgt ttccagcaga tcgggccgca ctagaaggag cggcaaaacc
     3541 ctctcggtct tctcgccaac gagaggcggc aaacccagca agtcgcccag cgcataaacc
     3601 tggccggtat agccagaata gtcgatgcgc aacgtctgcg aaaaaggcac gatcgcgtgc
     3661 tggtcgccaa cgcgcaccag tagtcccgga gtagccccac gcgcctcgta tggcgtatct
     3721 aactccggcg caatttgcag agaacgcgcg tcatcccgga tgagcagggt catgctcgcc
     3781 tgctgtcggt tcagcgcatc caggtgcagc agcgccagtt gcaactgcgc ggtagttgtc
     3841 gccacgtcag cgatggcctc gttgagcgag cgagacagca tgtgcgtttc ggtaaagtga
     3901 tcgagttcca actcatccca aagcatcgat tccgcgagtg atgcggacga tgcgaaaggg
     3961 gattgtgtcg cataggattg ctgccggggc tggtaatcat ggccggtacg ctgagccgat
     4021 tcgcgcagga tacgcgccac gagtgacgat ggcgcaagtt catcgatgct ggcctggcgc
     4081 gagccgttcg cctcagcggt agcgggcgat gaaatgggca gggcagccac caacgtttcg
     4141 atacgcctca aacgcgcctg tgacgcgcgc agttccgcca gtgccgtatc gacttctcgg
     4201 cgcgcatctt cgatctcatg ctgccgttcg gccagttgct cgacatgcga taacagcttc
     4261 gccaggtact cggcccattc cggcgcatgg agcgtgtcct ccatcatgtc accgcgccgc
     4321 gcatcgcgcc acgctcctat ccctcccact tcgtccggct catctcctgc gctcccttcc
     4381 gaccggcccg tactgtcgag cggcaaatgc agatcggcat atcgttcctc caacgtcagt
     4441 aagggaagaa tgctctcctg gccatgcgtc gcgacgcttt ccagcgtctc tctgagagca
     4501 tcgatagcaa agccgagcgc catcatcccg gattgccatt cgatttgctg gccgttcgcc
     4561 tgttcgatca tcgtttcaac agaatgggca atggtggaca tggccttgca accgattgcc
     4621 gccgacgtgc cgcgaatctt gtgcgcgcag cgccgcagcg ccgatagcgc tgaaattgcc
     4681 gacggcagca tgggagtcag cgcgggcagg atagcaagcg tttgatcctg ccgcaacagg
     4741 gccagtgcgc tctgcatatc ggcgatatct tcgtcaacct cggtcacgaa gatcgacaac
     4801 atctcctctt cttccccggc ctcgtcgagc gcgcccgtcc acccgtatac cgccgctatc
     4861 tctgacatcg ccgcggaatc atcgtcgctg gtcgtcgcga aatcttccgt tgcgagaaag
     4921 tcgcgaatga cgtccaggtc atcgggggtt aattcatttg aatcgagtgg caagggatca
     4981 gacattgtgt gctgccattc ctatctgccg cgcgatatcg gccagcagcg cctcgaagtc
     5041 cagcgcagcc gcccccgttt gctgtggctc cccgcctggc gcgaccgtct caccaacggc
     5101 aggcacaaaa aggccgagcg ccagttcacc gtaatggaga caaagcagca ggccaccgga
     5161 gcctattcta ctgtcggtat ggccgaccag gtacatttca agatcgacca ccgccattac
     5221 ctcgcctcgc cacgcggcca gaccgagcat ccagccaggt acggacggca gcagcgtata
     5281 gtgggaaggt gatgggagaa cttcttcaat gctggaaagc tgggtcaggc acattccccg
     5341 gctcagcatg atttccaggt attggccggc gtccgcacgc acaggagtac tggaccctcc
     5401 tcccgctaac tcgcgagcat aggcccagaa ttcctcgtca gacatatccc acaggcgttg
     5461 agccgggagt gcatcgaacg gcagcataca atcgtggcat taagaaaagc tgggcgcgcc
     5521 caggtgggta tggacaaccg aaataacttc ttgcgtgcga aatggcttgg tgatgtattc
     5581 cttcgcgccc gccagccgac ccttaaggcg atcaattacg ccgtctcgct tcgagagcat
     5641 gacgatcacc gtattgttga attgctcttt ggttttaaaa aaacgggcaa cttcgtagcc
     5701 atccattttg ggaaggccga tgtcgagaat tacgaggtcg ggcacggggc agcgtctttc
     5761 cttcaaggcg cgcagggctt caacaccatc ggcataacta attgatggta ttccttcacg
     5821 cctcaggctg gtttcaatga tcttccggac cgtcagtgag tcgtcaatga ccataaccag
     5881 tttgctcatc atttcttcct ctttctttcg ccacacttac taccttcaac atccgtaaaa
     5941 atatcacaac gctcatcttg aacgccgcgc tacaaacaag acggttcgat tcaatgacaa
     6001 catcctgttt gtacaaactc acaacaaccc gaaattcggg tggtctgggt tggtgtgcgt
     6061 agaacgtggt acgaatcaag gtcaggcata agcctatccc tggttcagat ttctctccct
     6121 caacaagagt accatacgaa aatgcatttc gcaacggaaa tgcataaaga tgtaattaaa
     6181 attgtcgata ttgttacaac aatgtcgaat ttgtaatgtt atcagagggt agtggcgcag
     6241 ggcgtaccct tgcggtcgcc ctccagaatc gagttgtata ccgacctacc gcgtcgtatt
     6301 gggtaatgta aacgtgaaga cgctgccctg gccggggacg ctttcggcct tgatacgccc
     6361 gccgtgcgcc tcaacgacat ggcgggcaat cgccagcccg aggccggttc ccgcgccacc
     6421 tggtccgatg aaatctgctt tcgaacgcgc gcgattgact ttgtaaaaac gctcgaagat
     6481 acgcggcaag tcttcaggag caatgcccac tcccgtatcg cgcacataga acgcctgcaa
     6541 attactgctc tgcgcgcacg ttccgatcac cacagcgccg ccggaggggg tgaacttgat
     6601 ggcattatgc gccaggttca ccagcacccg cgcaacctgt ttgacatcgg cggccaccag
     6661 tgtctgcccc tgctcgatct cagtgcgcag gctgacgcgg tgccgctgcg ccagtggcaa
     6721 catacgcgcc atcacctcgc gcaccagttg ctcggcctcc accggctcaa tcaccatcgg
     6781 tgtctgcccc gactcgatgc gcgacaggtc gagcagttcc gccaccagca tactcagatg
     6841 ctcgatctcg ttctcgatct tccccacgaa gacctgcgcc tgctccgggt cggtatcgat
     6901 ggcctcctcc agcgtctcca ccagcagccg caccgaagcc agcggtgtgc gtagttcgtg
     6961 cgaaatgttg gccacaaagt cgcggcgcgc gcgttccagg cggcgcagct cggtcaaatc
     7021 ctcgattacc accgcgaaat agcgtaatgt gcgcgccgtc ttgctatgcg ccagcggcct
     7081 gaccgagacc agccacgcgc tgccggacat tctctccgcc tcccccgtca gtcgctcctc
     7141 ccgcgccggc tgccgcgcct cgatgctccg ccgtatcatc tggagcaccc cctcatcgtg
     7201 gatcacccgc tcaagcaaca tcccctcgac aggttcgctc atcccaaaca gtgtcctggc
     7261 cttatcgttc gagaagcgga tcaccaggga ggcatcaaca atgaccaggg gaagcaacag
     7321 cgcatcaagc aactgccgga aaaaatgctc gtaatcgatc tgtgcgtgac tcatgcggaa
     7381 agtatagcac agtctatcat tcggggtgtt agttaggggt gagcgctatt ttttgcattg
     7441 atgaaattcc agggggcgac cacaagggtg cgcccttacc atgactacga gggggtcaag
     7501 catcatccat cgtatcatgg taagggcgc
//
LOCUS       DASZAK010000149         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_252311, whole genome shotgun sequence.
ACCESSION   DASZAK010000149 DASZAK010000000
VERSION     DASZAK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252311"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..>1501
                     /locus_tag="VGT44_02565"
     CDS             30..>1501
                     /locus_tag="VGT44_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV2579710.1"
                     /translation="MMHIPVKEAHATHGVREAARQILAAFREHNPAWQGELLPLDTLV
                     AWLDLDVATFSPDDYPEGTFGFLDPQERLIWLCRDLPPALHRFTLAHELGHAILHRQA
                     MPLEQGSQQDHAATRDDPCQEEDVRESVGGPIEQQSVEDMLGPGLSDTDYDPRSRREL
                     DANLFAAELLMPLEQVRARYLSRDIPPAKLADLFGVSPADMLNRLSELVIGDVATRDP
                     GDHKGPPPTSAQPSPLREREGRRQYDRYQQAAIEARTPALIVAGPGSGKTSTLIGRAE
                     YLIQEQGVEPQCLLALTFSRKAAQEMQERLQQALAPMTADHLEQAALFAEFPTVSTFH
                     AFCAGLLREYGSLVGLRPDFAFIDDAEGYFLLRRLGARLPLRHYQNLVNPAASFPAIL
                     SAISRAKDELTTPEMYAQLARAMLDGAQAMQETGEKQYEEELERAEKALEIAEMYALY
                     QRQLELQGDTDFGGLIMLAVQLLRDFPDVRLEVQERYQHIL"
BASE COUNT          295 a          459 c          474 g          273 t
ORIGIN      
        1 aatatcgcaa ctggcaagat tggacagtca tgatgcacat accggtaaag gaagcccatg
       61 cgacacacgg cgtcagagag gctgcccggc agatcctggc ggcgtttcgg gaacataacc
      121 cggcgtggca gggcgagctg ctgccgcttg acacgctggt cgcgtggctg gatctggatg
      181 tagcgacctt cagcccggac gactatccag aaggcacgtt cggtttcctt gatccgcagg
      241 agcgtttgat ctggctctgc cgcgacctgc cgcctgcgct gcatcgcttc acgctggcgc
      301 acgagctagg tcacgccatc ctgcaccgcc aggccatgcc gctcgaacag ggtagccagc
      361 aggatcacgc ggccacacgc gacgatccct gccaggagga agatgtacga gagagcgttg
      421 gcggccctat tgaacaacaa tcggtcgagg acatgctggg ccctggcctc tcggatacag
      481 actatgaccc gcgcagtcgg cgcgaactcg atgccaatct ttttgccgct gaactcttga
      541 tgcctctgga acaggtgcgc gcgcgctacc tatcgcgcga tatcccaccc gcgaagctag
      601 cagacctgtt tggtgtctca ccggcggaca tgctgaatcg gctgtcggag ttggtgatag
      661 gggatgtagc tacgagggac ccgggcgacc ataaaggtcc tccccccacg tccgcgcagc
      721 cctcgcccct acgggagaga gagggcagga ggcaatacga ccgctaccag caggccgcga
      781 ttgaggcgcg cacaccggcc ttgatcgtgg cgggtcccgg cagcggcaaa accagcacgc
      841 tgattggccg cgccgaatat cttatccagg agcagggagt agagccgcaa tgtctcctgg
      901 cgctcacctt ctcgcgcaag gccgctcagg aaatgcagga gcgcttgcag caggcgcttg
      961 ctcccatgac tgcagaccac ctggaacagg cggcgctgtt tgcagagttt ccgaccgtca
     1021 gtacctttca tgcgttttgc gcggggctgc tgcgcgaata tggcagcctg gtcgggttgc
     1081 gcccggattt cgccttcatc gacgatgccg agggctattt tttgctgcgt cgcctgggag
     1141 cgcgcttgcc gctgcgccat taccagaacc tcgtcaatcc agccgcctcg ttcccggcca
     1201 tcctcagcgc catttcgcgc gcgaaggatg aactgactac gccggagatg tatgcgcaac
     1261 tggcgcgcgc catgctggac ggagcgcaag ccatgcagga gaccggcgag aagcagtacg
     1321 aggaggaact ggagcgggcg gagaaggcgc tggaaattgc cgagatgtat gccctgtacc
     1381 agcgacaact ggagctacag ggcgataccg atttcggcgg cctgattatg ctcgccgtgc
     1441 agctcttgcg cgactttcca gacgtgcgat tggaggtgca ggagcgctac cagcatatcc
     1501 t
//
LOCUS       DASZAK010000150         4620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_252341, whole genome shotgun sequence.
ACCESSION   DASZAK010000150 DASZAK010000000
VERSION     DASZAK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4620
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252341"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VGT44_02570"
     CDS             complement(<1..289)
                     /locus_tag="VGT44_02570"
                     /inference="COORDINATES: protein motif:HMM:NF016413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV2579711.1"
                     /translation="MRYHIRERAWSLTEAFLVRDESGNPVFEIRGKFFHIGDDLVMYD
                     TYSGQALVQIKQRVLSLMPHYEIYRGGQLWASLHEQFRLFGEQFKIEGPDGM"
     gene            complement(326..994)
                     /locus_tag="VGT44_02575"
     CDS             complement(326..994)
                     /locus_tag="VGT44_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2579712.1"
                     /translation="MPTLVFLVDVDNTLIANDDAKLDFDEHIRVQLGEKLAERFWELY
                     ELARQQEEVVDIPLALKWLREETPLAELDEVTYAHVHSIFDNYPYFNKLYPHALETLH
                     HLRTLGLTVIVSDGDLYFQAEKIFRSDLAETVEGRVLIYKHKQQHLDEIMQRYPADHY
                     IMIDDKPQILADSQPIMGQTLTTVLVKQGKYANELPPNFSPDITVLHIGDLRTFTAEQ
                     FLSH"
     gene            1344..2705
                     /locus_tag="VGT44_02580"
     CDS             1344..2705
                     /locus_tag="VGT44_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579713.1"
                     /translation="MRTSRFWQSVKYIMIGALGMALTIRLFLALTRPRHSDQKKDAQS
                     QSVPLEPVSTHLAHRAYLQTYLNTSPLASTSVPGEKGSVEKKPGRTTTIVGVAMMLAV
                     IVGTFLYSGHIFAGVLFQSNSVAAAQPQLLRPTFERGIIYPQWSPDAYGAQDTVWQHG
                     IGPVKQQTGAQWIELPVLFSQASPSSTVITTVPSTPTISSFIEGIQWAHTLGYKVFFV
                     PLMQVRQTGMWSGSITFSGGQREQAWFDAYWKVIEPYVAAAAAQHVEQMAVGTELQTL
                     QQIVPDALWNRLIARVRGVYGNTLTYDMNWSSLVQSMPGWLENPALSYIGVSTYIPLL
                     NTPGRVDPAAMPVLWRDKVKSQLDALAVQLKKPVLITEIGYRNSADALYRTWEATSKA
                     HADPQEQAGAYEAALSNAFSDTHIAGTFFWGWDNVGMFAIKNQSAVQELLKWYSRTTT
                     QTT"
     gene            2917..>4620
                     /locus_tag="VGT44_02585"
     CDS             2917..>4620
                     /locus_tag="VGT44_02585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2579714.1"
                     /translation="MQPLPGAPAAEKQPSSPSAPDELVERLAGSYRAWLTAQTRQHRQ
                     TMDAEMLEYAERAMQAVQVKDRRIQTFAPFRYKYSALQTITRRQVHVLVGIVLLWMVA
                     LLWNWRATLAVTISAITIGYLAHLFLDGLLAIQSTRYDAEERIDENVIAALADADWPP
                     YTVLCPLYKEAQIVPQFVKAMKDMDYPADKLQILFLTEEDDQDTRRAIYALHLPSHFK
                     VVTVPDGSPRTKPRACNYGLIEATGQYVVIYDAEDVPDPLQLKKAVLTFANHGPDLAC
                     VQAKLNFYNPQQNVLTRWFTAEYSLWFDLILPGLQKIQLALPLGGTSNHFPVQTLRAL
                     GGWDAFNVTEDCDLGLRLSWFRLNTVIVNSTTLEEANSRMKNWIRQRSRWIKGYMQTY
                     LVHMREPQHYFSNRRYREFFSLQVVVGGKTAFLLLNPLMWVFFLVALALGKSFMQAYT
                     ALFPPAILYIGFFTLVMGNFFYAYSYLLGCVKREQYALVKWMLLIPVYWAMASAAAYM
                     ALYELCTRPHYWQKTIHGLHLNKPLEGAEMTGVDQKDLEWESLAQMAEGLSPVASAHG
                     LA"
BASE COUNT          996 a         1259 c         1323 g         1042 t
ORIGIN      
        1 tcataccatc tggaccttcg atcttgaact gctctccgaa caagcggaac tgctcgtgca
       61 aactggccca caactggccg ccgcgataga tttcgtagtg gggcatgagt gacaggacgc
      121 gctgcttgat ctgcaccagt gcctggccgg agtaggtgtc atacatcacc aggtcgtcgc
      181 cgatgtggaa gaatttgccg cgaatctcaa agacgggatt gccgctctcg tcgcgcacga
      241 gaaaagcttc ggtcaggctc caggctcgct ctcgaatatg gtagcgcatg attgggtatc
      301 ctccttcata cagtagcttt gatcgttagt gggaaagaaa ttgttcggca gtaaatgtgc
      361 gtaggtcgcc aatatgtaat acggtgatgt cgggcgaaaa gttgggagga agctcattgg
      421 catacttgcc ctgctttacc agcacggttg tgagtgtttg ccccatgatc ggttgagagt
      481 cggcgagaat ctgcggcttg tcgtcgatca tgatatagtg atcggcggga tagcgctgca
      541 tgatctcgtc caggtgctgc tgtttgtgct tgtagattaa gacgcgaccc tcgacggttt
      601 cagcgagatc gctgcggaaa atcttctctg cctgaaaata caaatcgccg tcagaaacga
      661 tcaccgtcag tcccagagtg cgcaggtggt ggagggtttc cagggcgtga gggtagagct
      721 tgttgaaata gggataattg tcgaaaatcg aatgcacatg cgcgtaggtc acctcatcca
      781 actccgcaag cggtgtctcc tcgcgcagcc acttgagggc cagagggata tcaacgactt
      841 cctcctgctg gcgcgccagc tcgtacaact cccagaagcg ctccgcgagc ttctcgccaa
      901 gttgcacccg gatatgctcg tcgaaatcca gcttcgcgtc gtcgttcgcg atcagggtat
      961 tgtctacatc caccaaaaaa accagcgtcg gcatagcttc ccctctttca atagctgtct
     1021 acttcatccg tgcgtcctgc tcccttaatg ttacagcctg ccgccccagt tggcaagagt
     1081 gtgcagtgtc atcctgagcg cagcgaagaa tctcggttcc cggccagtga gatcctgagc
     1141 gcagcgaagg atgacacatt tagaccgatc ctggtcggga accgtcgtct tcggcagtcc
     1201 aatcgaccat attgacgagg attcgcgccg atcaatcggc tgtgagcgcg gtaaaccggc
     1261 tcctactgta tttgccggag atatccgatt agagtagttg agaagacatc gcgaacgata
     1321 tttttgaata aggagtcgcc gctatgcgga catctcgctt ctggcaaagt gtgaaatata
     1381 tcatgattgg cgcgttgggg atggcgttga caattcgact ctttctggct ctgacgcgcc
     1441 ccaggcattc cgaccagaag aaggacgcgc agtcgcagtc cgtgccattg gagccggtct
     1501 cgacacacct ggcgcacagg gcgtatcttc aaacgtatct gaatacatcg ccgcttgctt
     1561 ctacgtcggt tcctggtgaa aaaggctctg tcgaaaagaa acctggtcgg acgacaacca
     1621 ttgttggagt tgccatgatg ctcgcggtca tcgtgggtac gtttctctac tccggtcaca
     1681 tcttcgccgg tgtgctgttt cagtcgaaca gcgtggcggc ggcgcagcca caattgcttc
     1741 gaccaacctt cgagcgtgga attatttatc cgcaatggtc gcctgatgcc tatggcgcgc
     1801 aggataccgt gtggcaacac ggtatcgggc cggtgaagca gcagacgggg gcgcagtgga
     1861 tcgagctacc ggtgttgttt tcccaggcgt cgccctcttc taccgtgata acgaccgttc
     1921 ccagcactcc cacgatcagc agcttcatcg agggtattca gtgggcgcac acgctgggat
     1981 acaaggtgtt ctttgtgcct ttgatgcagg tgcggcagac gggtatgtgg tctggcagca
     2041 tcactttttc cggcgggcag cgagaacagg catggtttga cgcatactgg aaagtgattg
     2101 agccatatgt cgcggcagcc gcggcgcagc atgtcgaaca gatggcggtt ggcaccgaac
     2161 tccagacatt gcaacagatt gtgccggatg cgctctggaa ccgcttaatt gcgcgagttc
     2221 gcggcgtcta cggtaatacg ctgacctacg atatgaactg gtcatcgctt gttcaatcca
     2281 tgccgggctg gttggaaaac cccgcgctat cctacatcgg cgtttctacc tatattcctc
     2341 tgctgaatac gccggggcgg gtagacccgg cagccatgcc cgtgctctgg cgcgacaagg
     2401 tcaaaagcca gctagatgcg cttgccgttc aattgaagaa gcctgtgttg ataacggaaa
     2461 ttggctatcg caacagcgcc gatgcccttt atcgcacctg ggaggcgacg agcaaggcgc
     2521 acgctgatcc acaggagcag gccggggcgt atgaagctgc gctatcgaat gctttctctg
     2581 atacgcatat cgcgggtacc tttttctggg gatgggataa cgtcgggatg tttgcgatca
     2641 agaaccaatc ggcggtacag gaactcttga aatggtatag tcgcacaaca acacaaacaa
     2701 cctgatagag ccaacccccc gtaggagccg gtttaccgtg ctcagagccg atttatcggc
     2761 gcgaatcctc gttgatcggc agggctggac tggccgataa atcggctctg agcacggtaa
     2821 accggctcct acggggttct gacgctcgtt tggctaccag tatttcctac accaaccatg
     2881 aagtggaaag gacaacgtat cgtggggact agattgatgc aaccattacc aggagcgcct
     2941 gccgccgaga agcagccatc gtcaccatca gccccggatg aactggttga gcggctggca
     3001 ggcagttacc gcgcgtggtt gacagcgcag acacgacaac accgccagac gatggacgcg
     3061 gagatgctgg aatacgctga acgcgcgatg caggcggtcc aggtcaaaga caggcgtatt
     3121 cagacatttg ccccctttcg ctacaaatat tccgcgctgc aaacaatcac caggaggcag
     3181 gtacatgttc ttgtaggaat tgtgctgttg tggatggttg cgttactctg gaactggcgg
     3241 gcgacgttgg ccgtgacgat ctccgccatc acgataggct acctggcgca tctcttcctg
     3301 gatggtctcc tcgccatcca atcaacgcgg tacgacgcgg aagagcggat cgatgaaaac
     3361 gtcatcgcgg ccctggccga cgcggattgg ccgccatata ccgtgctgtg tccgctctac
     3421 aaagaggcgc agatcgttcc acagtttgtc aaagcgatga aggatatgga ctatccggct
     3481 gacaaactac aaatcctctt tctcaccgag gaggacgacc aggatacgcg ccgggccatc
     3541 tacgcgctgc acctgccgtc gcatttcaaa gtggtgacgg tgccggacgg ttcgccgcgc
     3601 accaagccgc gcgcttgcaa ttacgggttg atcgaggcga ccggtcagta cgtcgtcatt
     3661 tacgacgccg aagatgtccc cgacccatta caattgaaga aggccgtgct gaccttcgcc
     3721 aatcatggac ccgacctggc ctgcgtgcag gccaagctga atttctacaa cccgcagcaa
     3781 aatgtactga cgcgctggtt tacggcggaa tattctctat ggttcgacct gatcctgccg
     3841 ggtctgcaaa aaatacagct ggcgctgccg ctgggtggca catccaatca cttcccggta
     3901 cagaccctgc gcgccctcgg cggttgggat gccttcaacg tgaccgagga ctgtgacctg
     3961 ggtctgcgcc tgtcgtggtt tcgcttgaac accgtcatcg tcaattcaac cacgctcgaa
     4021 gaagccaatt cgcgcatgaa aaactggatt cgccagcgtt cgcgctggat caaaggctac
     4081 atgcagacct acctggtgca tatgcgtgag ccgcagcact atttcagcaa caggcgctac
     4141 cgggaattct tctcgctgca ggtggtggtt ggcggcaaga cggccttctt actcttgaac
     4201 ccactcatgt gggtgttttt tctcgtggct ctggcccttg gcaagtcctt catgcaggcg
     4261 tatacggcgt tgtttccacc ggcgatcctg tatatcggct tctttaccct cgtgatgggc
     4321 aactttttct atgcctacag ctatctgctg ggttgcgtga aacgcgagca gtacgcgctg
     4381 gtgaagtgga tgttgttgat accggtgtac tgggccatgg ccagcgccgc ggcgtacatg
     4441 gccttgtacg agctgtgtac gcgcccgcat tactggcaga aaactattca tggcctgcac
     4501 ctaaacaaac cgttagaagg agcggagatg accggtgtcg atcaaaagga tctggagtgg
     4561 gaatcgctgg cgcagatggc cgaagggctt tcgccggtgg cttctgccca cgggctggcc
//
LOCUS       DASZAK010000151         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_500815, whole genome shotgun sequence.
ACCESSION   DASZAK010000151 DASZAK010000000
VERSION     DASZAK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500815"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VGT44_02590"
     CDS             <1..777
                     /locus_tag="VGT44_02590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2579715.1"
                     /translation="RAKELGVHVQQQIEVLGALLHADRISALVTNRGNVEADWFVNAT
                     NIWASRLSPRIGGMTLAVAPLKRYLYFLKPAQPVMDETTWQQLPMTIYGVGSHRGAHS
                     RPDHDLLLLGWAHETEPETQFSDGDQDRIDSPFRHDNGIDNYGYAVLAQVEEFAPSLA
                     NAGGLIATTCGFYGATPDGNPLIGRDAHQQNLVHAVGFSGHGLMHAPITALLVEAILS
                     GDVENGHVRMPAPFDHATIDLNTFTPGRAFERSRGEKLVL"
     gene            complement(772..1449)
                     /locus_tag="VGT44_02595"
     CDS             complement(772..1449)
                     /locus_tag="VGT44_02595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV2579716.1"
                     /translation="MTQQSSNLLQTQRDIEQRIQQAFAPEDEGLLYALASAREAGLPN
                     IQISPVQGKFLQVLAAACNARKILEIGSLGGYSGIWLARALPIDGRLITLEINPVHAA
                     VVHNSFAHAGVGDRTQVRVGRALDLLPTLESEAPFDLFFIDADKRPYPQYLEWALRLS
                     GPGSIIVADNCVRRGFQSLTASDEDSAGVFEYNRRIASDPRLASLALAMDDDYTDGFA
                     ISVVKYL"
BASE COUNT          301 a          445 c          448 g          307 t
ORIGIN      
        1 cgggcaaaag aactgggcgt tcacgtgcaa cagcaaatcg aggttctcgg cgctctgctc
       61 catgctgatc gcattagcgc gctggtgaca aacaggggaa acgttgaggc tgattggttc
      121 gtgaatgcta ccaacatctg ggcgtcgcgc ctcagtccac gcattggcgg catgacgctt
      181 gccgtagccc cgctcaaacg ttacctctat ttcctgaagc cagcgcagcc ggttatggat
      241 gaaaccacgt ggcagcagct tccaatgacc atctacggcg tcggaagcca tcgcggggcg
      301 cactcacggc cagatcatga tcttttgttg ctggggtggg cgcatgagac tgagccagag
      361 acacagttca gcgatggcga tcaggatcgc attgactccc catttcgcca tgataacggc
      421 atcgacaact atggctatgc cgtcctcgca caggtagagg aatttgctcc atcgcttgcg
      481 aatgcaggcg gtctcatagc gacgacctgc ggattctatg gcgcaacgcc tgacggcaac
      541 ccgttgatcg gtcgagacgc gcaccagcag aaccttgtcc acgccgtcgg attctcaggg
      601 catggcctga tgcacgcgcc gataaccgcg ctgctggtcg aagccatcct gtcgggcgat
      661 gtcgagaatg gtcatgtccg catgcctgcg ccatttgatc atgcgaccat cgacctcaac
      721 acattcactc ctggacgcgc ttttgagcgt tcgcgcggag agaagttggt cctatagata
      781 tttgaccacc gagatcgcga atccgtcggt gtagtcatcg tccatcgcca gcgccagaga
      841 cgccaggcga gggtcgctgg cgatacgccg gttgtattcg aagacgcccg ccgagtcctc
      901 gtcgctggcc gtcagggatt ggaacccccg tcgaacgcaa ttgtccgcta cgatgatact
      961 gccgggtccg ctcagacgca aagcccattc caggtattgc ggatagggcc gcttatcagc
     1021 gtcgatgaag aacaggtcaa aaggcgcctc gctctccaac gttggcaaga ggtcgagcgc
     1081 tcgcccgacg cgcacctggg tgcggtcgcc gacacctgcg tgggcgaagg aattgtgtac
     1141 gacagcagca tgtacggggt taatttccag ggtgatgagt cgcccgtcga tcggcagcgc
     1201 acgcgccagc cagatgccgc tatagccgcc cagcgagcca atttccagta tcttgcgcgc
     1261 gttacaggcg gcggccagga cttgcaggaa cttgccctgc acgggagaaa tctgtatgtt
     1321 cggcaaaccc gcttcccgcg ctgatgccag cgcgtaaagc aacccctcgt cttcgggagc
     1381 gaaggcttgt tgaatgcgct gctcgatgtc gcgctgcgtt tgcagcaggt ttgaggattg
     1441 ttgcgtcatg agtactcctt tgttctgctc tgccagggcg accgcgaggg tgcgccccta
     1501 c
//
LOCUS       DASZAK010000152         5637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_394365, whole genome shotgun sequence.
ACCESSION   DASZAK010000152 DASZAK010000000
VERSION     DASZAK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394365"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..1259
                     /locus_tag="VGT44_02600"
     CDS             147..1259
                     /locus_tag="VGT44_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2579717.1"
                     /translation="MMSKTPIGIIGCGNISSVYFKAPQIFDNLQVVACADIDMERARS
                     QAEKFGVPHVYTVEELLADPDIEIVVNLTVPNAHAEIAQKAIAAGKAVYGEKPLAIKR
                     ADGYAVLEAARARQVRIGNAPDTFLGGGFQTCIKAINDGQIGAPIAATAFVLNHGMEH
                     WHPDPYFFFQPGAGPLFDIGPYYITALIAMMGPIQRVTASMQTTFPERIVTSGQKYGT
                     KIPVNTPTHIAGIMEFASGAIGTILTSFDVWHHRLPFIEIYGTEGSLSTPDPNRFDGP
                     VYLRRAKDEAWREVSLTHGYTSNSRGLGIADMAYAMRSGRPHRANGEMAYHVLDTMES
                     LMQSAQEGRHVTLTSTCARPDPLNPGMHIWEEEQAE"
     gene            1364..2416
                     /locus_tag="VGT44_02605"
     CDS             1364..2416
                     /locus_tag="VGT44_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HEV2579718.1"
                     /translation="MSQIQGPALFLAQFAQDVPPYNTLENITSWAKGLGFLGVQIPVN
                     DKRLIDLDKAAESKQYCDDLKGKCNGLVITELATHLVGQLIAVHPAYDAQFDAFAPKE
                     LRGHPQERTEWAKAQMVKAMQASRNLGLQVMPTFSGALLWHTMYPWPQRPAGLVDLGF
                     QELAKRWQPILAKAEEYGIDLAYEIHPGEDLHDGITFERFLAATGNHPRAHILFDPSH
                     FVLQCLDYIGYLDCYKDHIRAFHVKDAEYHPSPRSGVYGGYQDWIDRPGRFRSTGDGQ
                     VDFKRVFSKLTQNGYTGWAVLEWECCIKDSVQGAREGAAFIRSMLIDVPTRAFDDFAA
                     GAQDIETNYRILGVQP"
     gene            2413..3558
                     /locus_tag="VGT44_02610"
     CDS             2413..3558
                     /locus_tag="VGT44_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2579719.1"
                     /translation="MKQDIRNRKLRAGIVGGGQGSFIGAVHRIAVELDGQAQVVAGAM
                     SADAQRAEASARAWHLQRSYASFEEMAETEAGLSDGIDLVMVTVPNHLHYPVARAFLE
                     HGIHVVCDKPMTFNLEQAQALVELVEQKQLVFALTHNYTGYPAVRHARHLVRSGAIGT
                     IRKVLVEYLQDWLMERLEQSGNKQAAWRTDPARSGIAGSIGDIGTHGENLLGYITGLR
                     LASVCADLTTFIEGRQLDDDANILLRLENGGKGILTCSQIAAGEENALSIRVYGSKAS
                     LEWHQMEPNTLLFKQPGQPRQALRTGLPYMSSEASAVTRLPAGHPEGYLEAFANIYRL
                     ALADIQRVESGESPAGGYPTVYDGLRGMQFITNVVESSQKGAVWVDF"
     gene            complement(3615..3995)
                     /locus_tag="VGT44_02615"
     CDS             complement(3615..3995)
                     /locus_tag="VGT44_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2579720.1"
                     /translation="MTTLQMKLELVAVPVSDVDRAKAFYTEKMGFHADFDQQVRDDLR
                     FVQLTPPGSACSIVIGVGITTMQPGSLEGLLMVVPDVRAVREELVKRGADISEIDVQD
                     WGSFVYFRDPDGNRWSLQQLPSRT"
     gene            complement(4117..5223)
                     /locus_tag="VGT44_02620"
     CDS             complement(4117..5223)
                     /locus_tag="VGT44_02620"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV2579721.1"
                     /translation="MDQNVQSSKAATSRRSFLRKGLVVGGAGAIGAGLLAGGLPAFAE
                     GSSSDLIEGDVAILRFLSAAEILETDLWQQYNELGGIQDSEVPGGSGSAPYIKALLQL
                     DGDMSQYIHDNTEDEFSHFTFINAYLVSKGADPVNLDKFRNLPSSKASGAQQIGRLTN
                     LMQLTVDTSYWTKYRSRTKNPDFGDSFAPAIPGLLNGQFPAIPRSDDDLHPADHIQAI
                     ANTAGFHFAFIEQGGTSLYPSLAQRVTHPEVLRILLSIGPTETAHFQTWHDKAGNAPA
                     LTDPTNGLVFPNLNAAPFGGEDFQTNLIMPEPTIFLSRKFPPVSIIRPTETEEAAMGA
                     VKALTDDGLFKGQSQEFFDVLRKLAEEADEARRG"
     gene            5392..>5637
                     /locus_tag="VGT44_02625"
     CDS             5392..>5637
                     /locus_tag="VGT44_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2579722.1"
                     /translation="MVGYRHQHEEGLRGSSSTMMWNIGGILAYISKSEGIQAGELVGS
                     GTVGFGCGLELGKLLQPGDIVELEVEGIGVLRNKLGTP"
BASE COUNT         1211 a         1600 c         1639 g         1187 t
ORIGIN      
        1 aattcaatcc tcctccacta taacacatac agacggcagg ccgcgcgctg tgccataata
       61 gaggggaaag aaattcgttg caggcaatcg accggcggta cttccgctcg tttgctttag
      121 ctagaaggga ccgttgatcc gcgattatga tgagcaaaac ccctatcggc ataataggtt
      181 gtggcaacat cagctccgtg tatttcaaag cgccgcaaat tttcgataat ttgcaggttg
      241 tcgcctgcgc tgatatcgat atggagcgag cgcgcagtca ggcagagaaa ttcggcgttc
      301 ctcacgtgta taccgtcgag gagttgctgg ctgatccaga cattgagatt gtcgtcaacc
      361 tgaccgttcc caacgcacac gctgaaatcg cgcagaaagc gatcgcggct ggcaaagcgg
      421 tttacggtga aaagccattg gcgatcaagc gagcggatgg gtacgccgta ctcgaggctg
      481 ctcgcgctcg acaggtgcgc attggcaatg cgccggatac ttttcttgga ggtggttttc
      541 aaacctgcat caaagcgatc aacgatggtc aaatcggcgc ccctatcgct gctacagcat
      601 tcgtgctgaa tcacggcatg gaacactggc atcccgaccc atatttcttc tttcaacccg
      661 gcgccggccc cctgtttgac ataggtccgt attatattac cgcgctcatc gccatgatgg
      721 gtcccatcca gcgtgttact gcttcgatgc agacgacctt tcctgaacgc attgttacca
      781 gcgggcagaa atatggcacg aagattcccg tcaatacgcc aacgcatatc gcgggcatta
      841 tggagttcgc gagtggcgcg atcggcacaa ttctcaccag tttcgatgtc tggcatcatc
      901 gcctgccatt tatcgaaatc tacggaacgg aaggcagcct gagtacgccc gatcccaatc
      961 gcttcgatgg tccggtctac ctccgccggg cgaaagatga agcatggcgt gaagtctctc
     1021 ttacgcacgg ctacaccagc aactcgcgcg gactgggcat cgccgatatg gcctatgcga
     1081 tgcgttcggg tcgcccgcac cgcgccaatg gagaaatggc ctaccatgtc ctcgacacca
     1141 tggaatccct catgcaatcc gcgcaggaag gcaggcatgt gacgctgacc agcacatgcg
     1201 cccgtccaga cccgctcaat ccggggatgc acatttggga ggaagagcag gcagaataat
     1261 tgaacgacgg cagcaaggaa gacgagggcg accattgcgg tcgctctggg gcttgcactg
     1321 taatgtccgt aattcgtgct ttgcgataga aaggcaggca gacatgtctc aaattcaagg
     1381 accggctctg tttttagcgc aattcgccca ggatgtccct ccctataaca ccctggaaaa
     1441 cattacatcc tgggcgaaag gactgggctt tctcggcgta cagattccgg tcaacgacaa
     1501 acgactgatc gacctcgata aagcggcgga gtcaaaacag tactgcgatg acctcaaggg
     1561 aaagtgcaac gggctggtca tcaccgaact ggccacgcac ttggtcggac aactcatcgc
     1621 ggtacacccg gcctacgacg cgcaatttga cgcattcgcc cccaaagagc tacgcggaca
     1681 cccgcaggaa cgcacggaat gggccaaagc tcagatggtc aaagccatgc aagcctcgcg
     1741 caacctgggc ttgcaggtca tgccgacctt ctcaggcgcg ctgctctggc acaccatgta
     1801 tccctggcca caacgccctg ccggtctggt ggacctgggc tttcaagaac tggcgaaacg
     1861 ctggcagcct atcctcgcta aggcggaaga gtacggcatc gacctggcct acgaaattca
     1921 tccaggcgag gacctgcacg atggtatcac ctttgaacgc ttcctggcgg ccaccggcaa
     1981 ccatccgcgc gcgcatatct tatttgaccc atcccacttt gttctgcaat gcctggacta
     2041 catcggctac ctggactgct ataaagacca catccgcgcc ttccacgtca aggatgccga
     2101 gtaccatccg tcgccccgct cgggcgtcta tggcggctac caggactgga tagatcggcc
     2161 tggacgcttc cgcagcaccg gagacgggca ggttgacttc aagcgcgtct tctccaagct
     2221 gacgcagaat ggctatacag gctgggccgt gcttgaatgg gagtgctgca tcaaggactc
     2281 ggtgcagggc gcgcgcgaag gcgccgcgtt tattcggagt atgctcatcg atgtcccgac
     2341 gagagccttt gacgatttcg ccgctggcgc gcaggacatc gagaccaatt atcgcattct
     2401 gggggtgcag ccatgaaaca agatatacgc aaccgaaaat tgcgcgccgg tatcgttggc
     2461 ggcggccagg gttcattcat cggagccgtg catcgcatcg ccgttgaact ggatggacag
     2521 gcccaggttg tggcaggcgc gatgtccgcc gatgcgcagc gcgcggaagc ctcggcgcgc
     2581 gcctggcatt tgcagcgctc ttatgcgagc ttcgaggaga tggccgagac cgaagccggg
     2641 ttgtccgatg gcatcgatct tgtcatggta acggttccca atcacctgca ctaccccgtg
     2701 gcgcgcgcct tcctggagca tggcattcac gtcgtctgcg ataagcccat gaccttcaac
     2761 ctggaacagg cacaggcgct ggtggaactg gtcgagcaaa aacaactggt gttcgccctg
     2821 acgcacaact acaccggcta tccggcagta cgccacgcgc gccacctggt gcggtctggc
     2881 gcgattggca cgatccgcaa agtgctggtg gagtacctcc aggactggct gatggagcgt
     2941 cttgagcaga gcggcaacaa gcaggcggca tggcgcaccg atccagcccg gtcgggtatc
     3001 gcgggttcca ttggcgatat cggcacgcat ggcgaaaacc tgctgggata catcaccggt
     3061 ttacgcctgg cttccgtctg cgcagacctg acgaccttca tcgagggtag acaattggat
     3121 gatgatgcca atatcctgct acgcctggaa aacggcggta agggcatcct gacctgctcg
     3181 cagatcgcgg caggcgaaga aaatgccctc agcatccgcg tgtatggcag caaagccagc
     3241 ctggagtggc atcaaatgga gccaaacacg ctcctcttca aacagcctgg acagccgcgc
     3301 caggcgctgc gaacgggtct gccctacatg agcagcgagg ccagcgccgt tacccgcctg
     3361 ccagcagggc atcccgaagg ctacctggaa gccttcgcca atatctatcg cctggcgctg
     3421 gccgatattc agcgcgtcga atcaggcgag tcaccggcgg gaggataccc aacggtctat
     3481 gatgggctgc gcggtatgca atttatcacg aacgttgtgg agagttcgca aaaaggcgca
     3541 gtctgggtag acttttaacc gccctggcgt cgtcctcctc gcctgaagcc gccgaacgag
     3601 cctctgcatg taagctaggt gcgactgggg agttgctgca aggaccagcg attgccgtca
     3661 ggatcacgaa agtatacaaa agacccccag tcctgcacgt cgatctcact gatgtcagcg
     3721 cctcgtttga ccagttcctc gcggacggct ctcacgtccg ggaccaccat gagcaaccct
     3781 tcgagcgatc cgggctgcat agtggtaatg cccaccccaa tcacgatgga gcaggccgaa
     3841 cctggagggg tcagctggac aaagcgtaga tcatcccgga cttgctggtc aaaatcagcg
     3901 tgaaagccca ttttctcggt ataaaaagcc tttgcgcggt caacgtctga gacgggtacc
     3961 gcgaccagtt cgagtttcat ctgcaaggta gtcatcgtta ctatcctcct tcaaggtgct
     4021 tgacggttct tctccttcaa gtataccctg gaaggaggac agtaacgacc ctcgttattt
     4081 cgtcccttcc atctcccatt caaccctggc ggaacattag ccgcgacgcg cctcgtctgc
     4141 ttcctccgcc agcttacgaa gcacatcgaa gaactcctgt gattggcctt tgaacagccc
     4201 atcatccgtc aaggctttca ccgcgcccat agcagcctct tctgtttcgg tcgggcgaat
     4261 gatcgaaacg ggcgggaact tgcggctgag gaagatggtc ggttccggca taatcaggtt
     4321 ggtctggaag tcttccccgc caaagggggc ggcgttgagg tttgggaata ccaggccatt
     4381 cgtgggatcg gtcagggccg gcgcattgcc tgccttgtcg tgccaggtct gaaagtgcgc
     4441 tgtctcggtc gggccaatgc tgagcaggat gcgcagcacc tcggggtgag tgacccgctg
     4501 cgccagcgac gggtagaggc tggtaccgcc ctgttcgatg aatgcgaaat ggaaacccgc
     4561 cgtattggcg atagcctgga tgtgatctgc ggggtggagg tcgtcgtcac ttcggggaat
     4621 cgccggaaac tgtccattca gcagccctgg aatggccgga gcgaacgagt cgccgaaatc
     4681 ggggttcttc gtgcggctgc ggtactttgt ccagtaactg gtgtctaccg tgagctgcat
     4741 gaggttagtg agccgcccga tttgctgcgc gccgctcgcc ttgctgctcg gcaagttgcg
     4801 gaatttatcc aggttgacgg ggtctgcgcc ttttgagaca agataggcgt tgatgaaggt
     4861 gaagtggctg aactcatcct cggtattgtc gtggatgtac tgcgacatgt cgccatccag
     4921 ttggaggaga gctttgatat aaggagcgct tccgctgccc cctggaactt cgctgtcctg
     4981 gatgccgccg agttcgttgt actgctgcca tagatcggtt tcgaggattt cagcggcaga
     5041 caggaaccgg agaatggcta catccccttc tatgagatcg ctgctcgatc cctcggcgaa
     5101 ggcgggcaat ccaccggcca gcaggccagc gccaatggcg cctgctcctc ctaccacgag
     5161 tcctttccgt aagaaggaac ggcgactggt ggcagctttc gatgattgta cgttctggtc
     5221 catgatcttt tctttctgcg actgttgcgc gaggtattca gtgattttta gctgaatatg
     5281 gcaatgttca gctaagggcg caagctggtc ctggctagcg tccttgatga actacggatg
     5341 tttgttaccg gtggagatcc ccaaagcatc gacgtgctgt tgtagaatac gatggtaggt
     5401 tacagacatc agcatgagga gggtttgcgc ggctcaagtt ccaccatgat gtggaacatt
     5461 ggcgggattc ttgcgtatat atctaagagc gagggtattc aggcgggcga gctggtcggc
     5521 tccggcactg ttggctttgg ctgcggactg gaactcggca aactcctcca accaggcgat
     5581 attgtggaac tggaggtgga gggtataggc gtactgcgca ataagctggg aacgcct
//
LOCUS       DASZAK010000153         5382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_607606, whole genome shotgun sequence.
ACCESSION   DASZAK010000153 DASZAK010000000
VERSION     DASZAK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5382
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607606"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VGT44_02630"
     CDS             <1..928
                     /locus_tag="VGT44_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2579723.1"
                     /translation="KEGRRMLHTDAVLEVKGLRMRYGDTVVLDGVDFTMCRGEVLVLL
                     GPNGAGKTTIIEILEGFRGRSGGSVSILGADPAFGDETWRARVGIVLQSWRDHSGWQV
                     WELLHHLGGYFAPYSTDERKRPYDTDALLELVGLTDQASKKVRDLSGGRRRRLDVAIG
                     LVGNPELLFLDEPTVGFDPQARREFHDVIHRLSEREETTILLTTHDLDEAEKLSDRIL
                     ILAGGAIVANGSAGQLAKDMSNQTEVTWTQDGQQHSQRTSQAVAFTRALLTRHPDGIS
                     DLEIRRASLEDTYMAIVQTHEKQQNPKEEGKK"
     gene            925..1770
                     /locus_tag="VGT44_02635"
     CDS             925..1770
                     /locus_tag="VGT44_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579724.1"
                     /translation="MSPMTNAVRLGVVRGWIEFQVYYLKDLQSMVGTVIIALILVGVL
                     WFERATNIEGVSLALLTLPGLLAMLIANEGFSNVARFLSSHREDGTLLRAKAIPQGMV
                     GYLIARVVVSLLSIILNLAIVFALSLFIVPGLANLDGGRVLTFLWVLALGLLATAPIG
                     AVIGSLVKSSGAALGLTFLPLVILVAISGIFYPISALPAWVQWIAQVFPVYWLGLGLR
                     SVFSPATAAALEFGGTFRPLATFLVLAAWTVAGLLLAPPVLRRMARRTSGNEMEAGKQ
                     RSIRR"
     gene            1870..2949
                     /locus_tag="VGT44_02640"
     CDS             1870..2949
                     /locus_tag="VGT44_02640"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEV2579725.1"
                     /translation="MTNRDIAHQRLHNQGIASAAFEKASDVVRWFGAVQAQDYQGALW
                     AVGLRMRNAMAADIEQAIADGVIVRLHPMRGTWHFVAAEDIRWLLALMASGTIDRSAY
                     YYRQLELDEATFAKSNAVFAQTLAGGKQLTRRELAAALEQAGISTQGLRLTFLLGRAE
                     VDGVITAGARRGKQFTFALLDEMIPVSRMLERDEALAELARRYFTSHGPATVQDFIWW
                     SGLAAADARAGFAMVQSHFVQEVIDGQTYWLSPSTLTAKVASPTAYLLPPYDEYTVAY
                     KDRSAVLDPAYAEQTRNGIFNPVIAVDGQIAGTWKRVIKKEKVLITSDIFTPLDEAET
                     SALVATAHRYGSFFGGSADITIQEA"
     gene            3063..3389
                     /locus_tag="VGT44_02645"
     CDS             3063..3389
                     /locus_tag="VGT44_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEV2579726.1"
                     /translation="MMIIVTTEGVANYRIVETRGQVFGVVVRSRGLGGNIMAGLRSLG
                     GGEIKEYTSLLEDARRHAIDRMVQNATAMGANAVVRMQFDSSEIGTTMSEIVAYGTAV
                     VVEPER"
     gene            3399..3692
                     /locus_tag="VGT44_02650"
     CDS             3399..3692
                     /locus_tag="VGT44_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579727.1"
                     /translation="MLRWVIIGYGLLYLIGAAVLLFLVHATLWLVVYLAVNGFILVSA
                     LLLERKRYRTQVDRTQGRWQPTGERFVDPTTERLMEVFYNSATGERDYREATE"
     gene            complement(3823..>5382)
                     /locus_tag="VGT44_02655"
     CDS             complement(3823..>5382)
                     /locus_tag="VGT44_02655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEV2579728.1"
                     /translation="EEYPDSQPEDADRTVKRLPTSELWGPWYVPPQDSAENRPVAQFK
                     KPGDLTEPPRAVTVDGEGVDPQAVYREHLLPLPSNPSYQGYPSGPVMQPGYPPYSANG
                     FASNAAPYQPYAPYAPYQTYPPPPPRPKRDGYLFGVGIAALVGSSLVLLAGLIALLFF
                     IVLVVAPTSRVPADQQFMLVVSLLAFALIGVIGGSFGLYHSIRSVFLRKLSAGFALPT
                     FWLFVLLYLGVIGAGYALHVQGMDVTNEPLTIFLILMAGLTPALAVLALGNRRLGFPK
                     GASWPTTWRRFALAIVSGATLGIVIAGLLELVFTLVLVRGQNVDPFVCLTNPDAAPCQ
                     NPQVYQLLLIAVAVIAPLVEETVKPLAVVLLIGRVRSAAEAFVLGLSCGIGFDLIETS
                     GYISSSYTGWLDTALVRTGAGLLHGLGAAMVALGWYYLTHPGKRRVPKALGCWLYAVA
                     QHAIWNGSWGTLLLSGSVGQFFSNTLTLGSVAIPYYDFINIVEAIIMLAFFLYMTGRL
                     RKTPSPVVEAV"
BASE COUNT         1177 a         1390 c         1654 g         1161 t
ORIGIN      
        1 gaaagaagga agacggatgc tgcatacaga tgcagtatta gaagtgaagg ggctgcggat
       61 gcgctatggc gacacggtcg tcttagatgg ggtagatttt acgatgtgcc ggggtgaagt
      121 gctggtcctg cttggtccca atggggctgg gaagacgacc atcattgaaa tcttagaagg
      181 ctttcgtgga cgctctggcg gaagcgtgag cattcttggt gctgatcctg cctttgggga
      241 tgaaacatgg cgtgcaagag tagggattgt gttgcagtcg tggcgtgatc actcagggtg
      301 gcaagtgtgg gaactacttc accaccttgg cggctacttt gccccgtact caacagatga
      361 gcgtaaacgt ccttatgaca cggacgcttt gcttgaactg gtgggcctga cagaccaggc
      421 cagcaagaaa gtccgcgacc tctccggcgg gcgacgtcgc cgactggatg tcgctatcgg
      481 gctggttggg aatcccgaac tgctctttct ggacgagccg accgtggggt ttgacccgca
      541 ggcgagacgc gagtttcatg acgttattca ccggctttcc gagagggagg aaacgaccat
      601 tttactgacc acacacgatc tggatgaagc agaaaaattg agtgatcgca ttctgatttt
      661 agcaggaggc gcgattgtcg caaacggaag tgccggccaa ctcgcaaaag acatgtccaa
      721 tcaaacagag gtcacctgga cgcaggatgg gcagcagcac agccagagga ccagccaggc
      781 tgtagcattt accagagcgt tgctcacccg gcatcccgat gggattagtg acttagaaat
      841 ccggcgcgcg agcctggaag acacctatat ggccattgtg caaacgcacg agaaacaaca
      901 gaacccaaaa gaggagggaa agaaatgagt ccaatgacga atgcggttcg cctcggcgtt
      961 gtccgtggat ggattgagtt tcaggtttac tacctgaaag acctgcaatc aatggtgggg
     1021 acggttatta ttgcgctcat cctggtgggt gtcctgtggt ttgagcgggc aacgaacatt
     1081 gaaggcgttt ctctggcgct tttaacgctg ccgggactgc tagccatgct gatcgccaat
     1141 gaagggttta gtaatgtggc aaggttcctt tcttcccaca gagaggacgg aacgttactg
     1201 cgcgcaaagg cgattcctca aggaatggtt ggttacttga ttgcgcgagt ggttgtgtcc
     1261 cttctttcaa tcatcttaaa tctggcgatt gtattcgccc tgagtctctt cattgtacca
     1321 ggactggcca atcttgatgg gggacgtgtc ctcacctttt tgtgggtgtt ggcgcttggt
     1381 ctactcgcaa ccgccccgat tggcgcagtc attggatcac tggtgaaaag ttcgggcgct
     1441 gctcttggtc tgacgtttct tcctctggtg atcctggtag caatatcggg aatcttttat
     1501 ccaatcagcg cgcttcctgc ctgggtgcaa tggattgccc aggtgtttcc tgtctactgg
     1561 ttagggctgg gccttcgttc ggtgttttcc cctgcaacag cagccgcgct tgagtttggc
     1621 gggacgtttc gaccgctggc aacctttctt gtccttgctg cctggacggt tgccggtttg
     1681 ctgcttgcgc ctcctgtgtt acgccgcatg gcgcgacgta cttcggggaa tgaaatggag
     1741 gccggaaaac agcgctctat tcggaggtag ctctacgatc ctgacgaaaa gatgaacgag
     1801 gtatgattct gtcctccttc gatggtatgc taaagtatat gaaatacttg caggcaagaa
     1861 ggagctatca tgacaaaccg ggatattgcg caccagcgac tacataatca aggcattgcg
     1921 tcggcggcat ttgagaaggc gagcgatgtt gtgcggtggt ttggcgctgt ccaggcccag
     1981 gattaccagg gagcgctgtg ggcagtcggg ctgcgtatgc gcaatgcgat ggcggcggat
     2041 attgagcagg ctatagcaga tggcgtgatt gtgcgcctgc atccgatgcg aggaacgtgg
     2101 cattttgtcg cggcagagga tattcgctgg ttgctggcct taatggcatc agggacgatt
     2161 gacaggagcg cctactacta tcgccaactt gaattagatg aggcgacttt tgctaaaagc
     2221 aacgctgtct tcgcgcaaac gctcgcaggc ggaaagcagc ttacccgtcg tgaactggca
     2281 gccgcgcttg agcaggccgg tatttctacg caggggctgc ggctcacgtt tctcctgggc
     2341 cgcgccgagg ttgatggcgt gattactgct ggcgcgcggc gcggcaagca gtttaccttt
     2401 gcgctgcttg atgaaatgat cccggtttcc aggatgcttg aacgcgacga ggcgctggcc
     2461 gaactggcgc gccgttactt tacgagtcat ggaccagcaa ccgtgcaaga ttttatatgg
     2521 tggtcgggac tggcagctgc ggatgccagg gccggctttg cgatggttca gtcgcacttt
     2581 gtacaggagg ttattgatgg ccagacctac tggctttctc catcgacgct aacggcaaaa
     2641 gttgcgtcgc caacagctta tttgctgccg ccatatgatg agtataccgt tgcttataaa
     2701 gatcgcagcg ccgtacttga cccggcctat gccgaacaga cgagaaacgg tatcttcaat
     2761 ccagtcatcg ctgtagatgg tcaaatcgca ggcacctgga agcgcgtcat aaaaaaggag
     2821 aaagtgctta tcacgtcaga catctttact ccgctagacg aagccgaaac gagcgccctg
     2881 gttgctacgg cacatcgcta tggttcgttt tttggggggt cggcggatat taccatccag
     2941 gaggcgtaac cactccaact ccttgacttt ggaactattt tgcgttatta tttcaatata
     3001 tgttgaagca ttgaaatcga gaatgcttac gctctcaagc atacatatcg acgaaggaga
     3061 aaatgatgat tatcgtaaca actgaagggg tcgccaacta tcggattgtg gaaaccaggg
     3121 gacaggtctt tggcgttgta gtgcgcagcc ggggtctggg gggcaacatt atggcgggct
     3181 tgcgttccct gggaggcggc gagatcaagg aatacacctc cctgctggag gatgcgcgcc
     3241 ggcacgcgat tgaccgcatg gtccaaaacg ccacagctat gggcgccaat gcggttgtgc
     3301 ggatgcagtt tgattcctcg gagattggca ccacgatgag tgaaatcgtg gcctatggca
     3361 cggcagtggt ggtcgagccg gagaggtagg gagcgggtat gctgcggtgg gtgattattg
     3421 gctatggcct gctctatttg attggagcgg ccgtgttgct gttccttgtc catgccacgc
     3481 tctggctggt tgtgtatctg gcggtgaatg gcttcatcct ggtgagcgcc ctgctcctgg
     3541 agcgcaaacg ataccgcact caagtggatc ggacccaggg gcgctggcag cccaccggcg
     3601 agcgtttcgt tgatcccaca accgagcggc tcatggaagt gttctacaat agcgcaaccg
     3661 gcgagcgcga ttatcgagag gcgaccgaat aatttgtagc cccggcatgg cgaccgcaag
     3721 ggatcgccac tacgataacg aagtgatcgg ctaacaccat cgttatcgta gtggcgatcc
     3781 cttgcggtcg ccatgccgtc caatccctat tggttcagat ttttacacag cctccactac
     3841 aggcgatggc gtcttgcgca gcctgcctgt catataaagg aagaaggcca gcatgatgat
     3901 tgcttcgacg atgttgataa agtcgtaata agggatggcg acggagccga gcgtcagcgt
     3961 attggagaag aactggccta ccgagccgga cagcagcagc gttccccacg agccattcca
     4021 gatggcgtgt tgcgccaccg cgtagagcca gcagcccagg gccttcggca cgcgacgttt
     4081 gccgggatgg gtcaggtagt accaaccgag cgcgaccatg gccgcaccga ggccatgcag
     4141 caggcccgcg ccggtacgca ccaacgcggt atccagccag ccggtgtagc tggaactgat
     4201 gtagccactt gtctcgatca ggtcaaagcc aataccgcag gagaggccaa gcacgaaggc
     4261 ttccgccgcg ctgcgcacac gcccgatcag gaggacgacc gcgagcggct tgacggtctc
     4321 ttcaacgagc ggcgcgatga cggcaacggc gatgagcagc agctggtaaa cctgcggatt
     4381 ctggcacgga gctgcatcag gattagtcag gcacacaaag gggtcaacat tctggccgcg
     4441 caccagcaca agggtgaaga ccagctccaa cagaccagct atcacgatgc cgagtgttgc
     4501 gccgctcaca atcgccagcg caaacctgcg ccaggtcgtc ggccacgacg cgccttttgg
     4561 aaagcccagc cgccggttgc ccagcgccag tactgccagc gcgggcgtca gtcccgccat
     4621 taagatcaaa aagatggtga gtggctcgtt ggtgacgtcc atgccctgga cgtgcagggc
     4681 gtagccagcg ccgatcactc ccaggtagag caggacgaag agccagaagg tcggcagggc
     4741 gaacccggct gacagcttgc gcaggaagac cgagcgaatg ctatgataca ggccgaagct
     4801 gccgccgatc acgccgatca gcgcgaaggc cagtaaagag acaacgagca taaactgctg
     4861 gtcggcaggc acccggctgg tcggcgcgac aacgagtacg atgaagaata acagcgcgat
     4921 cagtccggcg agcagcacca gcgacgaacc aaccagcgca gcaatgccga ctccgaacag
     4981 gtacccatcg cgttttggcc tgggtggagg gggcgggtat gtttgatacg gcgcataagg
     5041 cgcgtagggc tggtaaggtg cggcattgga agcaaagcca ttggcgctat agggcgggta
     5101 accaggctgc atcaccgggc cggaaggata tccctggtac gatggattcg acggcaaagg
     5161 gagtagatgc tcgcgataga ccgcctgtgg gtcaacgccc tctccatcaa ctgtaactgc
     5221 cctgggcggt tcggttagat ctcccggttt cttgaattgc gcgaccgggc gattctccgc
     5281 gctatcctgc ggcggcacat accagggtcc ccacaattca gatgtgggca gcctcttcac
     5341 cgtcctgtcg gcatcttccg gctgcgaatc gggatactct tc
//
LOCUS       DASZAK010000154         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_430043, whole genome shotgun sequence.
ACCESSION   DASZAK010000154 DASZAK010000000
VERSION     DASZAK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430043"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1009)
                     /locus_tag="VGT44_02660"
     CDS             complement(59..1009)
                     /locus_tag="VGT44_02660"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2579729.1"
                     /translation="MTDVRYLVTGGAGFIGSHIAQALLDRGETVRVFDNLATGRQANL
                     LALEGRAHAIQGDLRDMEAIKAAAQGVEVIFHQGALASVPRSIADPVASLESNVNGTQ
                     NVLLAARDAGVRRVVYASSSSVYGDTPTLPKREDMPTNPLSPYAIHKLTGELLCGVFT
                     RIYGLETVALRYFNVFGPRQDPNSQYAAVLPRFLTALLEKRRPVVFGDGEQTRDFTYV
                     ENVVQANLLAATAPDAVGRAMNIGCGEQISLNSVLRLAGAMLGEPVEADYRETRAGDV
                     KHSLADIRLAGQLLGYQPAVKFQEGLSRTLAALQKDAQRL"
     gene            1219..2532
                     /locus_tag="VGT44_02665"
     CDS             1219..2532
                     /locus_tag="VGT44_02665"
                     /inference="COORDINATES: protein motif:HMM:NF026235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEV2579730.1"
                     /translation="MIFSFLISKRAAWMVGFMLMLFLSSCSSTQVGTNPTPTRSPSRT
                     PTPLPSPATPYPILKGTRPFIDTFNNIHLLGNFDYNIDNPIAIAKYYDFFWGVSVNDV
                     RALRAGNPNIFLTYYIPFHREVGTFTNQNATHALSYWKAIHPDWILYQCDRVTPAYEF
                     GDPNIPLDFSNPALVAWQVQTYALPASENGYDGIAADNVNLQNYFGACGVYVNGQWKQ
                     LYSGQVDDPLWRADVLTWLSRMQLALHHLAHPLALIPNLSLGSIPPTDPIVSQIIQHV
                     DGVVDEDGFTADSRGDISDTNWVQLVQFMTTVQQAHIPFYVVNQFATASVSRADIQWA
                     LASYLMGKGHSAAIFISTYQGYGGDTRYPEYNAQVGSPVGAMFASQHVYWRNYSAGVS
                     LVNPSATQTYTVTLNAAHRYVDLYGVVVQKQVTLPPHTGLVLLSR"
BASE COUNT          562 a          779 c          710 g          594 t
ORIGIN      
        1 gagggcgcaa ttcattgcgc ccctacattt cgccattcca attgcgtgtg ataggccact
       61 agaggcgctg cgcatctttc tgcagggcgg caagagtgcg tgagagaccc tcctgaaatt
      121 tgaccgctgg ctgatagccc aggagttggc cggcgaggcg aatatcagcg agactgtgtt
      181 tcacatctcc ggcgcgcgtt tcacggtagt cggcttccac cggctcgccc agcattgccc
      241 cggcgaggcg cagcacgcta ttgagcgaga tctgctcgcc acagccgata ttcatggcgc
      301 ggccaacggc atccggggcg gtcgccgcga gcaggttggc ctgcacgacg ttttcgacgt
      361 aggtgaaatc acgagtttgt tcaccatcgc cgaagaccac cgggcggcgt ttctcaagca
      421 gcgccgtcag gaagcgcggt aggactgccg cgtattgcga attggggtcc tggcgtgggc
      481 cgaagacgtt gaagtagcgc agcgcgaccg tctcaagccc gtagatgcgg gtaaacacgc
      541 cacagagcag ctcgcccgtc agtttgtgta tcgcgtaggg cgacagggga ttggtcggca
      601 tatcttcgcg cttgggtaaa gtcggcgtgt ctccatacac agatgaggag ctggcgtaga
      661 cgacgcgccg cacacccgcg tctcgcgccg ccaggaggac attctgggtg ccgttgacgt
      721 tgctttccag cgaggccacc gggtcggcga tggaacgcgg caccgaggcc agcgctccct
      781 ggtgaaaaat gacttcgacc ccctgcgcgg cggctttgat ggcctccata tcgcgcaaat
      841 caccctgaat agcgtgcgcg cggccttcca gcgcgagcag gttggcctgt ctgcctgtcg
      901 cgagattgtc gaacacgcgc acggtctctc cccgatcaag cagcgcctgc gcgatatgcg
      961 aaccaatgaa cccggctccg cctgtaacga gatatctcac atcggtcatc tgaatgcatt
     1021 aagcgctcca atccgaaacg aagtagagcg catactcctt tctctgtcga tcgttgtcct
     1081 ttttaactct accagaattc atgaattttg aacagtgacg gctgttcaac cctgtgctat
     1141 actagcttcg ataacggaac cggatctcct gaatatctgg gggatagatg ttcaaacatg
     1201 tataagcagg agtatgttgt gatattctcg tttcttatta gcaagcgagc tgcctggatg
     1261 gtcggcttca tgctaatgct cttcttgagc agttgcagca gcacacaggt tggcacgaac
     1321 cctactccca ctcgttcccc ttctcgcact ccgacaccgc ttccttcgcc agcaacaccg
     1381 tatcccattt tgaaagggac acgccctttt attgatacgt tcaacaatat tcatctcctg
     1441 ggaaactttg actacaatat agacaatccg atagctatcg caaaatacta cgacttcttc
     1501 tggggtgtgt ccgtaaacga tgtccgggca ctgcgcgcag gcaaccccaa catcttcctg
     1561 acatactaca ttccctttca tcgggaagtc gggacgttta ccaaccagaa tgcgacgcac
     1621 gccctgagtt actggaaggc tatccacccc gactggatac tctatcaatg cgaccgggtc
     1681 actcctgcct acgaatttgg cgatccgaat ataccgcttg atttctccaa tccagcgctg
     1741 gtggcctggc aagtgcagac gtatgcgctg ccggccagcg agaacggcta tgacggtatt
     1801 gcagcggaca atgtgaacct gcaaaactac ttcggcgcgt gcggcgtgta cgtcaacggc
     1861 cagtggaagc agctttatag cgggcaggtc gacgaccctt tatggcgagc agatgtcctc
     1921 acctggctct cgcgaatgca actggctctg caccacctgg cccatccatt agcgttgatc
     1981 cccaatctct cgctgggcag tattcctcca accgatccaa tcgtatcgca aatcatccaa
     2041 cacgtagatg gggttgtcga tgaagacggc ttcacagccg attcgagagg cgatatttct
     2101 gatacgaatt gggtgcaact tgtccaattc atgacgaccg tacagcaggc gcacatacca
     2161 ttctatgtcg tcaaccagtt tgcgaccgcc tccgtgagtc gcgcggacat ccagtgggcg
     2221 ctcgcttcgt acttgatggg caaagggcat tctgccgcaa tcttcatttc tacctatcaa
     2281 ggctatggag gcgatacgcg ctaccctgag tacaatgccc aggtgggaag tcccgttggc
     2341 gcaatgttcg cctcacagca tgtctactgg cgtaattatt ctgctggagt gagcctcgtc
     2401 aatcccagcg cgacgcagac ctacaccgtt accctcaatg ccgcgcacag gtacgtcgat
     2461 ctctacgggg ttgtcgttca gaaacaagtg accctgcccc cgcatacagg cctggtgctg
     2521 ttgtccagat gacctgtagg ggcgcaatta tgaatattca ccatcaaagt tggccatccc
     2581 tggttgtcat cctgagcgca gcgaaggatc tcggtccccg gcgcgtcaga tccttcgctg
     2641 cgctc
//
LOCUS       DASZAK010000155         1677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_430083, whole genome shotgun sequence.
ACCESSION   DASZAK010000155 DASZAK010000000
VERSION     DASZAK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1677
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430083"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1548)
                     /locus_tag="VGT44_02670"
     CDS             complement(52..1548)
                     /locus_tag="VGT44_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HEV2579731.1"
                     /translation="MPTFETGQIFDRYRILSWLGSGTSGESYEAEDAMLLRKVTLKLI
                     YPQEALPDSARRQFFREMQGISALNHPSIASVLDYGEVNGRLYLARRYVSNGSLLGTE
                     GRLWFRPPLNVNDAVCYAHQIAGALHSIHVRGYVHGAVTFTNLLVLRSPGLNGQLSDA
                     PFLLADAGLAQFVRRFGQSRQPLLPVSAAPEQIGKRVTPASDQFALAVLLYTWLAGRP
                     PYTGTPEEVERLKLTESITPLSTINAHVTFEQDGILLRALSVFPEDRYPTVLAFADSM
                     LSSLADQSPIPSPVAADDASTSIIEEPAQKEAGDIAMGPEQQPTDNLERLEPVTDDLP
                     ARIDTQPFDSDLQPEREAEPIIPDTPVLANAAEEPGVPTSVYGRVIISSPYSDDPLEV
                     SLENEEMTVGRAGSSDILLDYDNLTSRHHALFRREGSVYVLYDRHSANGVFVNGQKIS
                     AEEGYTLVDGDHVSIGNYELIFRATPRDGIDFHELAVAEEVQEYSIHP"
BASE COUNT          338 a          454 c          507 g          378 t
ORIGIN      
        1 gagggcgcaa ttcattgcgc ccctacacgc catttgaact ttgacaaaac actacggatg
       61 aatcgagtat tcttgtacct cttctgccac agccaactca tggaaatcaa taccatctct
      121 gggtgtggcg cggaagatca attcgtagtt gccgatgctg acatgatcgc catcgaccag
      181 cgtataaccc tcctccgcgc tgatcttctg cccgttgaca aagacgccgt tcgcgctgtg
      241 cctgtcgtac aggacgtaga cgctgccttc gcgccggaag agtgcgtgat ggcgcgaggt
      301 caggttatcg taatcaagca ggatatcgct tgaaccggcg cgtcccacag tcatttcctc
      361 gttttcgagc gagacctcga gtgggtcgtc cgaatagggc gatgagatga taacccggcc
      421 atacacggat gttggcacac ctggttcctc tgcggcgtta gccagtacag gtgtatcggg
      481 tatgatcggt tcagcttctc tttccggttg caaatcagaa tcgaatggct gagtatcaat
      541 gcgcgctgga agatcgtctg taactggctc aagtcgttcc aggttgtcgg ttggctgctg
      601 ttcgggaccc atcgctatgt cgcctgcttc tttttgagca ggctcttcga tgatggaggt
      661 actggcgtcg tcggctgcga caggcgaggg aatcggcgac tggtctgcga gagagctgag
      721 catggaatcg gcaaaggcca gcacggtagg atagcgatct tcagggaaga ccgagagggc
      781 gcgcagcaag ataccgtcct gctcgaaggt gacatgcgca ttgatggtgg agagcggggt
      841 gatcgattcg gttaatttca gccgttcaac ttcttcaggt gtgccggtat agggcgggcg
      901 accggccagc caggtgtaga gcagcacggc cagggcaaac tgatcgctgg caggagtaac
      961 gcgcttgccg atttgttctg gcgcggcgga caccggcagc aggggttggc ggctctgccc
     1021 gaaacggcgc acgaattgtg ccagtccggc gtccgccagc aagaatggcg cgtcactgag
     1081 ctggccgttc agaccgggac tacgcagcac aagcaggtta gtgaaggtca cagccccatg
     1141 cacgtagccg cgtacatgaa tagagtgcag cgccccggcg atctgatgag cgtagcagac
     1201 ggcatcgttt acattgaggg gcgggcgaaa ccagagccga ccttcggtgc cgagcagcga
     1261 gccattgctg acgtagcggc gcgcaaggta gagcctgccg ttgacctcgc catagtccag
     1321 gactgacgca atcgaaggat gattgagcgc gctgatcccc tgcatctcgc gaaagaactg
     1381 gcggcgcgcg gagtcgggca gcgcttcttg cgggtaaatc agtttgagcg tcactttacg
     1441 caggagcatc gcgtcttcgg cttcatagct ttcccccgat gtcccactgc ccagccagct
     1501 caggatgcga taccgatcaa atatctgtcc cgtttcaaat gttggcatag gttcctcact
     1561 ctttcgcatt atcgccgctg ctcatcgttg gcacagccgc atcagtccca ttggttgcgt
     1621 tggtagtatc tgtttcgttt atctcgtcgg tcgcgcctat tgcgttcgtc gtgttta
//
LOCUS       DASZAK010000156         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_287281, whole genome shotgun sequence.
ACCESSION   DASZAK010000156 DASZAK010000000
VERSION     DASZAK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287281"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="VGT44_02675"
     CDS             complement(<1..1322)
                     /locus_tag="VGT44_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV2579732.1"
                     /translation="MLEVNDFNAIRISLASPEQIRGWSYGEVTKPETINYRTLKPEKD
                     GLFCERIFGPTKDWECYCGKYKRVRFKGIVCDKCGVEVARSKVRRERMGHIELASPVS
                     HIWYFKGTPSRIGLLLDLSPRNLERILYFALYVVTHIDEEARQQELMRIDEELVNLDQ
                     NLDEKVVERIDSLKEKRDSEIQEAFNRITENQQDIEARREQELETARQAESETLAGLR
                     AAMDHAAEQDIVFSPTDRVIVKQGDEATSKDIETLERVAEVWRNDIAENAHHKIEEIK
                     TGSSHEAERLRVDYQGQIDSAQTQLETQRVDKKHRWEQLRRDLEGLKKMDLIQENRYR
                     ELRDSFGLNVFRAGMGAEAVRELISALNMDKLAGDLRHEIGSTVGQRRKKATKRLKVV
                     EAFRKSNTSPEWMILTVLPVLPPDLRPMVQLDGGRFATSDLNDLYRRV"
     gene            complement(1338..>1709)
                     /locus_tag="VGT44_02680"
     CDS             complement(1338..>1709)
                     /locus_tag="VGT44_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV2579733.1"
                     /translation="PYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAANILQEILTV
                     KSDDVVGRVKTYEAIVKGENIMEPGVPESFKVLVKELQSLGINVEVLNEDEQKIQFVE
                     DTSNDVMPELGINISGFEGDQ"
BASE COUNT          347 a          503 c          405 g          454 t
ORIGIN      
        1 atcacgcgcc ggtagagatc attcaaatcg gatgtcgcga aacgcccgcc atcgagctgc
       61 accattggcc gcagatccgg cggtaatact ggcaagaccg ttaagatcat ccactcgggc
      121 gacgtgtttg acttgcggaa agcctcgacg actttcaatc gcttcgttgc ctttttgcgc
      181 cgctgaccaa cggtggagcc gatctcgtgc cgcaggtccc cggccagttt atccatgttc
      241 agcgcactga tcagttcgcg caccgcttcc gcgcccatac ccgcccggaa tacgttaaga
      301 ccaaaagagt cgcgtaattc acgatagcgg ttctcctgga tgaggtccat cttcttgagc
      361 ccctccaggt cacgtcgcag ttgctcccag cgatgcttct tgtcgacacg ctgtgtctct
      421 aactgcgtct gggcggagtc aatttgaccc tggtaatcta ccctcaagcg ctccgcttcg
      481 tgactgctac ccgtttttat ttcttcgatt ttatgatgag cattttcggc gatatcatta
      541 cgccagacct ctgcgacccg ttcaagcgtt tcaatgtctt ttgatgtagc ctcgtctccc
      601 tgcttgacaa tgacacgatc cgtcgggcta aagacaatgt cctgttcggc tgcatggtcc
      661 attgctgcgc gtaacccggc gagcgtttcg ctttcggcct gcctggccgt ctcaagttcc
      721 tgctcgcggc gcgcctcgat atcctgctga ttttcagtga tgcgattgaa cgcctcctga
      781 atttcgctat cacgcttctc cttcaagcta tcgatgcgtt cgacgacctt ctcatccaga
      841 ttttgatcga gattgacaag ttcctcatct atgcgcatca attcctgctg ccgggcttct
      901 tcgtcgatat gtgtgacaac atagagggcg aagtagagaa tgcgctccag gttacgtggc
      961 gagagatcca gcaagagacc aatacggctc ggcgtcccct tgaagtacca gatgtgactt
     1021 acgggggagg cgagttcgat atggcccatg cgctcgcggc gcacctttga gcgagccact
     1081 tcaacgccgc acttgtcgca cacgatgcct ttaaagcgca cgcgcttata tttaccgcag
     1141 tagcattccc agtccttagt tgggccgaag atgcgttcgc agaacaaccc gtccttctcc
     1201 ggcttgagtg tgcggtagtt gatggtttcc ggtttcgtta cctcaccata actccaacca
     1261 cgaatctgct cagggctggc aaggctgata cgtattgcat taaaatcgtt tacttcgagc
     1321 atgaatacct cctggtacta ttgatcccct tcgaaaccgg atatattaat gcccagctct
     1381 ggcataacat cattcgaggt atcctcgaca aactgaatct tctgttcatc ttcgttgagt
     1441 acctcgacat taataccaag gctttgcaat tctttgacta agactttgaa ggattccggc
     1501 actcccggct ccataatatt ttcacccttg acaatagcct cgtaggtctt cacgcgccct
     1561 acgacatcgt cggatttgac ggtcaagatt tcctgcagga tgttcgctgc cccataggct
     1621 tcaagcgccc atacttccat ttctccgaaa cgctgcccgc cgaattgcgc cttgccgcca
     1681 agcggctgct gcgttatcag gctgtatgg
//
LOCUS       DASZAK010000157         9188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_252593, whole genome shotgun sequence.
ACCESSION   DASZAK010000157 DASZAK010000000
VERSION     DASZAK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252593"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VGT44_02685"
     CDS             <1..510
                     /locus_tag="VGT44_02685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2579734.1"
                     /translation="LAAHPAARFVVAGGKAEQVRKLRQLAEEWDVADKIRLVGQRALE
                     EMPRYMALADILLSPRSKGTHTPLKLYTYLRSGKPILATDILAHTQILTPEVAWLVPA
                     TPQDLAQGAIDLLDNRQRAEALGRRAREVAGKLYSWAAFLEKNRQVYAEVMGPAWDGA
                     AQPAEAVAH"
     gene            538..1497
                     /locus_tag="VGT44_02690"
     CDS             538..1497
                     /locus_tag="VGT44_02690"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEV2579735.1"
                     /translation="MRCLVTGVAGFVGSHLAERLLADGHEVCGIDAFIDYYPRRIKEL
                     NLERLRDWERFTFVEDDILSVNLFRLLEGVDWIFHQAAQAGVRSSWGHEFARYTECNI
                     LATQRLLEAAAHVRRLRRFVYASSSSVYGEPTTLPVTEQVMPQPVSPYGVTKLAAEHL
                     CVLYHRNFGVPTVALRYFTVYGPRQRPDMAFHRFCKAILDHEAIRVFGDGLQTRDFTY
                     ISDVVEANIQAAGADGVAGEVMNIAGGARVTVRSVLEMLQEVSGAPIAIDYDNRQPGD
                     VLDTFADTTRARQLMHYQPRVGLREGLAAEFEQIVSLYERVRR"
     gene            1536..2873
                     /locus_tag="VGT44_02695"
     CDS             1536..2873
                     /locus_tag="VGT44_02695"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV2579736.1"
                     /translation="MSETQSRLPGIFDPPGVSDARYARQSQVTPGAQRVPGRISLRDY
                     GAFQADISTLATHRIVAIKDMLPITPAPVAYPKGRFAGVIKIIPFQTKEMRAQGSATE
                     ELRLEPGERANGQRASRKKAVSFGLRCAITLLMFGLLGKSISWPTLLAGLFHMRLGMV
                     SLSLVVGAGGIVLSAYQWRSLLSGEGIHLDLANLINLYIVGIAFSHFLPTGMGGDAVK
                     AFYVGRESGNNAGSASSVVLCRVIGFVGMLLVAIPALVIWHDHFRLELITTFALLSLL
                     VGGMIGGALAAVAWLPGLLKRVEKGRRVFTKVALVGEALLAAMKRPRFISVALAYGIA
                     FWALAILNCYAYGNALGIHVPSYFYMVVVPLIALVSFLPVSINGFGLRESAYVYAFST
                     MHVAPATALLLALLQDTQALLFGMGGGLVYFTLLSKTNQVRTRHAGRGRERAA"
     gene            2949..4229
                     /locus_tag="VGT44_02700"
     CDS             2949..4229
                     /locus_tag="VGT44_02700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV2579737.1"
                     /translation="MMNKQLEKSQFQPLPNRSARLPIETVDTCSLWAAHQQEAPFARH
                     QTPVHQAAGQVAATPPRVARTPRRPAWQKNRRLGIMVALGIVSLACLVSLVSLAAWRF
                     MTPTMPDVTLYHVSMKRVSLSIGGGGIAYPVQRLDISYPFTAHVLSVFVKPGDRITPN
                     QSLVQIDLSQVNGQRLSTLQAQVAQAYQDMLASQSYYYSVLKLGNPIVVAQAQQQYTA
                     AQAQYDTLVAEEQAPDLHQGRIDSTIAGVVTAVNVFPGQLVGANQIMLTIFDEASVIV
                     RVQVPLSNYGQVQINQPAQATPSALPGQSFNGTVIAIIPNVNPQSGTFEAWIMIPNTN
                     GTLLPGSNIYVRIQNTVNALVVPRLAVLNPDLDSTVYVVRQQQVHLVHVQVSGYAGDS
                     VLIASGLQANDLIVLVGLDSLQDGQAVHVTGIES"
     gene            4354..5703
                     /locus_tag="VGT44_02705"
     CDS             4354..5703
                     /locus_tag="VGT44_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579738.1"
                     /translation="MFLKRVFATDRGKRASGMATARGMAGCLMLAACILPWMRAPFGQ
                     VYSAWQLPVDAGWQVRTSYFNYGVLCLICACAEFWAAWRAYREVPPMRGRVMGSAPGL
                     ITVGLFLAQYLLNDRGAIDQLAQQNNQMLLIAGHFGYSHTPEIFPLKAMGIDLSNIGT
                     RFQLLVDQLQAGAFVLIPALCLMLYSGGTKRAALPGKRAGRALLIGAGLLFVVVIAGR
                     PLAGMVAQNLADSAIAQGDYSGALQWLDRAVFFDPLMSQEDFYHIERGQAELFLYPAR
                     QSDDSRAYVASLLDQQKDYQGAYQLLSNTSQANRAAPWVVDQSSAALESLVESAHAAQ
                     LQYLPQKPQSIIAKDSAALPWLQVLLQTDPGNVYAQYMLGRVDYDLQEYAASKARLLA
                     SLQLVSDEAYRSSVYTYVAFSDDRLGNYVEGRSMLLEAVQLDPEYRNDVARGAISGL"
     gene            5729..>9188
                     /locus_tag="VGT44_02710"
     CDS             5729..>9188
                     /locus_tag="VGT44_02710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2579739.1"
                     /translation="MISTHTTTQAIPQTPEVYVAETRATETRRARPFKALAGLVLALA
                     CLGASWFAYQSELAPQPKDFQPAWHDAQWIGASDGNATTAYYRYATTLEVATDNAFVT
                     IAASQVFSFYVNGVLIGSNSTDFANYPRSYIYEVTPSLVLGVNVFAIRVDNLDGQAPL
                     LRASFGTVIGGIVTYYGTGDGAGANWMATTQTTQVYPQDTSIIVAATAWTGRTFNAAS
                     WLPAQPVTSSTQSMLTSQSPSLSVNPQLYEQPVAAHWVSTGVGQDGYFVRQVSLPQGT
                     SMSWLRIAATGTANVFINGSLIFTWNYHRTSFSQITVNKLTGKTKVRHIPELVLGVYD
                     VSPFLHAGVNTVAIHISLQNVPGLRNIQVTEAPALSMDVLAGDGQGDFTWIGSPDGWQ
                     ASHQQAPAWINGGAAVHDWTSPTFTGRPDGYSIVYVSRSSELTRGQQAVQSVPVEQAI
                     MVILLSIAAVLGLWLLVSLTIARRFVHSASTALELMSLAYLPALAGEGLLIVISKEPQ
                     VAQPFPYTTLWGLMLLAGVAAGYLLISLSVGLRRATGRPHDERDALEGRPQGYAPIGV
                     NLTTKSVILSAAKDLTRRGPRSFAALRMTFDDPSRNTIAQEGQLKLTLMGYAPRVQRP
                     LDWLKRHWPIVVLLVATMPLVFYDLGYEAYWQDELVSYLVAKSILYNGLPMLPSGFLY
                     PKGELYSYLLALFMSIFGDQGGGPRVISGVEYLLTLALVYYVGCRFFDRRVALVAALM
                     LAFSPHELIWARQMRMYQQAQLLTLLTTYLFYRAVQERHRVSLVYLATGVLLVTYLSH
                     EETFIILPALVLCLLVVGYIVKEERHRLPWFFYQKHWWIAASIAVGVIGTQLLITKVT
                     HPPVLGTDTTVRPMIQLTTNNIPYYLNMLFTPTSNDPSNSTLVSNIPSSIMLDSCLML
                     LGCILAMKSNRLALRFTALFFVVSFLTLVFVLTMQADRYFYPLAPFYYLLAAFAFVKI
                     ARLVWVFAKTRLASPNSRPSASIIKWLVGLSAALALVSVLLAPMFPLNNYNLYVSRLF
                     NLPYYHHFGDYDEAGLYIRQHMRQGDIVISIIPDSIVMFYAGQSDYFFSVNHALFLFE
                     QDSHIVDTYSGKVALLRQSDLDAVLDMHARVWLFSANNHYQNEVLKHFILPSDFHVVY
                     SGADSIV"
BASE COUNT         1753 a         2527 c         2817 g         2091 t
ORIGIN      
        1 ctggcggccc acccggcagc gcgctttgtc gttgcgggtg gcaaagcgga acaggtgcgg
       61 aaactgcggc aactggccga ggaatgggac gttgccgaca agatacggct ggtcggccag
      121 cgtgcgctgg aggaaatgcc ccggtacatg gcgctcgcgg acatcttgct cagcccgcgc
      181 agcaagggga cgcatacgcc gctcaagctc tacacctacc tgcgctccgg caagccgatc
      241 ctcgccaccg atatcctggc gcacacacag attttgacgc ctgaggtcgc ctggcttgtg
      301 cctgcgacgc cgcaggacct ggcgcaggga gcgatcgact tgctcgacaa caggcagcgc
      361 gccgaagcgc tgggaagacg cgccagagag gtcgccggta agctctatag ctgggccgcg
      421 ttcctggaaa agaatcgcca ggtctatgcc gaagttatgg gtcccgcgtg ggacggggca
      481 gctcagccag ccgaggcggt tgcacattaa agatcagatg agaaagaaag aaaatacatg
      541 cgttgtttag taacgggagt cgcggggttt gtgggatcgc acctggcgga gcggctcctt
      601 gccgatggac acgaagtgtg tggcatcgat gcctttatcg actactaccc gcgcaggatc
      661 aaggagttga accttgagcg gctgcgcgac tgggaacgat ttacctttgt cgaggacgat
      721 atcctgtcgg tgaacctgtt tcgcctgctc gagggtgttg actggatttt tcaccaggcg
      781 gcgcaggcag gggtgcgttc cagttggggg catgagttcg cgcgctacac ggaatgcaat
      841 atcctggcga cgcagcgctt gcttgaggcg gcggcgcatg tcaggcgttt gcgccgcttc
      901 gtgtatgcct cgtcctcctc ggtatatggc gagccgacga cgctgccggt gacggaacag
      961 gttatgccgc agccggtttc gccctacggc gtgacaaagc tggcggctga gcatctgtgc
     1021 gtcctctatc accgtaattt cggagtgccg accgttgcgc tgcgctactt tactgtctat
     1081 gggccccgcc agcgaccgga tatggccttt catcgcttct gcaaagcaat cctggatcac
     1141 gaggctatcc gcgtgttcgg ggatggcctg cagacgcgcg atttcaccta tatctctgac
     1201 gtggtcgagg ccaacatcca ggctgccggc gccgacgggg ttgccggtga agtcatgaat
     1261 atcgcgggtg gggcgcgtgt taccgtgcgc tccgttttag agatgttgca agaggtcagc
     1321 ggcgcgccta tcgcgataga ctacgacaac cggcagcccg gcgatgtgct ggatactttc
     1381 gccgacacga cccgcgccag gcaactcatg cactatcagc cgcgcgttgg gttgcgagaa
     1441 gggcttgctg ctgaatttga gcagattgtg tcgctttatg agcgtgttcg gcggtgagga
     1501 tacgtggcgg tcgacatgta aacgggggtg gttagatgag cgagacacaa tcgcgattac
     1561 cgggtatttt tgatcctcct ggcgtctccg acgcgaggta cgcccggcaa tcacaggtta
     1621 cgccgggcgc gcagcgtgtt cccggtagga tcagtctgcg cgattatggg gcgtttcaag
     1681 cggatatctc cacgcttgct acgcaccgta tcgtagcgat caaagatatg ctgcccatca
     1741 caccggcccc ggtcgcctat ccaaaggggc gctttgcggg cgtcatcaag atcatcccgt
     1801 ttcaaacaaa agagatgcgc gctcaaggca gcgctaccga ggagctgcgc ctggagccag
     1861 gggagcgcgc gaacggccag agggcgtcgc gcaaaaaggc cgtgtcgttt gggctgcgct
     1921 gtgctatcac actgctgatg tttggattgt tgggcaaatc gatttcctgg ccaacgctcc
     1981 tggcggggct gttccatatg cggttgggca tggtcagcct gtcgctggtg gtgggcgcgg
     2041 gcggtattgt gctgagcgcg taccagtggc gcagcctgtt gagcggggag ggcattcacc
     2101 tggacctggc gaacctcata aatctctaca tcgtcggcat tgctttcagt catttcctgc
     2161 caaccggcat gggtggagac gcggtcaaag ccttctatgt cgggcgcgag tcggggaaca
     2221 acgcaggctc ggcgagttcg gttgtgctat gccgggtcat cggcttcgtc gggatgctgc
     2281 ttgtagcgat ccccgcgctg gtgatctggc acgatcactt tcgcctggaa ctgattacca
     2341 ccttcgcgct gttgagcctg ctggtgggcg gcatgatcgg cggcgcgctg gcagcggtcg
     2401 cgtggctgcc ggggctgctt aaacgggtag agaaaggcag gcgcgtcttc acgaaggtgg
     2461 cgctggttgg cgaggcgctg ctggctgcca tgaaacgccc gcgtttcatc agcgtagccc
     2521 tggcgtatgg tatcgctttc tgggcgctgg ctattttgaa ttgttatgcc tatgggaatg
     2581 ccctggggat acacgtccca tcctatttct atatggtggt tgtgccgtta atcgcgctgg
     2641 tctcgttcct gccagtctcc atcaacggct tcggactgcg ggaaagcgcc tatgtttatg
     2701 cgttctcgac tatgcatgtc gcgcctgcca cagcgctgct gctggcactc ttgcaggata
     2761 cgcaggcgct gctcttcggc atgggcggcg gcctggtcta tttcacactg ctcagcaaaa
     2821 caaaccaggt acggacacgg cacgcagggc gtggccgcga gcgggcagca tgaaaggcga
     2881 aaagctcggc gtggcgaccg aacatgaaga ttacaaataa cccggcccta ttctggaggt
     2941 tcgcatatat gatgaataaa cagctggaaa aaagccagtt tcagccttta cccaatcgat
     3001 cagcccggtt gcctatcgag accgttgata catgctcgct ctgggctgcg caccagcagg
     3061 aagcgccatt cgccaggcac cagacgccgg tccatcaagc tgcgggtcag gtagccgcga
     3121 ctcctccacg agtggcgcgg acgccccggc gaccggcatg gcagaagaac aggcgcctgg
     3181 ggatcatggt tgcgctgggg attgtgagtc tggcctgcct ggtgagcctg gtgagcctgg
     3241 ctgcctggcg tttcatgacg ccaacgatgc cggatgtcac gctctaccat gtcagcatga
     3301 aaagggtcag cctgtctatc ggaggcggcg ggattgccta cccggtgcag cgcctggata
     3361 tctcttatcc gtttacggcg cacgtgcttt cggtttttgt gaagccgggg gacaggatca
     3421 cgccgaatca gtcgctggtg cagatcgacc tatcgcaggt gaatgggcag aggctttcaa
     3481 cgttgcaggc ccaggttgct caggcatatc aggatatgct ggcatcgcag tcgtattact
     3541 attctgtcct gaagcttggc aatccgattg tggtggccca ggcgcagcag caatacacgg
     3601 cggcccaggc gcagtacgac acgctggtcg ccgaggagca ggctccagac ctgcatcagg
     3661 gcagaatcga ctcaacgatt gccggcgtgg tgacagcagt caatgtcttc cccggtcaac
     3721 tcgtcggcgc gaatcaaatc atgctgacca tctttgatga ggcgagcgtg atcgtgcgtg
     3781 tgcaggtgcc gctctcgaat tatggccagg tgcagataaa ccaaccggcg caggccacgc
     3841 cgtccgctct gccaggccag agcttcaacg gcacggtgat cgccattatt ccaaacgtca
     3901 atccacaatc cgggacattt gaggcgtgga tcatgatccc gaatacgaat gggacactgc
     3961 tgccaggctc aaatatctac gtgcgtattc agaataccgt caatgcgcta gttgtgccgc
     4021 gcctggccgt gctgaatcct gaccttgact cgaccgtcta cgtggtgcgg cagcaacagg
     4081 tgcatcttgt ccatgtccag gtctcaggtt acgcgggcga ctccgtgctg atcgcttccg
     4141 gtttacaggc caacgacctg atcgtgctgg taggtctcga ctcgctgcaa gatggacagg
     4201 cggtgcatgt cacgggtatt gaaagttagt acaccgtcca ggttgatgtg gcgtgtaggg
     4261 gcgcaattca ttgcgcccgg gaggaggcac gtgaccagga acacgggcgc aatgaattgc
     4321 gcccctacac agaaacagag aagggggtat ggcatgtttc tcaagcgggt tttcgcgacg
     4381 gatagaggta aacgggcgag tggcatggcg acggcgcgtg gcatggcggg gtgtttgatg
     4441 ctcgcagcct gtatcctccc ctggatgcgc gctccgtttg gccaggttta ttctgcctgg
     4501 caacttccgg ttgatgctgg ttggcaggtt cgaacgagct actttaacta tggtgtactg
     4561 tgtcttatat gcgcgtgtgc ggaattctgg gctgcctggc gagcttaccg ggaggttcct
     4621 cctatgcgag gtcgcgtgat gggaagcgcg cctggcttga taacggtagg tctatttctt
     4681 gcccaatatc ttttgaacga tcggggcgct atcgatcaac tggctcaaca gaataaccag
     4741 atgctgctca tcgccggaca cttcggctat tcgcatacac ccgaaatctt tcccttgaaa
     4801 gcgatgggga ttgatctttc caatataggg acgcgctttc agttgctggt tgatcagttg
     4861 caggccgggg cctttgtttt gattcccgcg ctctgcttga tgttgtacag cggagggacg
     4921 aagcgcgccg ctttgccagg caagcgagca ggccgggcgc tgttgatcgg cgcgggcctg
     4981 ctcttcgtgg tggtgatcgc cggtcggcct cttgccggga tggtcgcgca gaacctggcg
     5041 gatagcgcga ttgcgcaggg ggattatagc ggcgcgctgc agtggctgga tcgagcggtg
     5101 tttttcgatc ctttgatgag ccaggaggac ttctatcata ttgagcgcgg ccaggcggaa
     5161 ctattcctgt atcccgcgcg gcagagcgac gatagccgcg cgtatgtcgc atctctactg
     5221 gaccagcaaa aagattacca gggcgcctac cagttgttgt cgaacacctc gcaggccaat
     5281 cgagccgcgc cctgggtggt cgatcagagc agcgcagcgc tggaaagcct ggtcgaatcg
     5341 gctcacgcgg cgcagttgca atatttgccg caaaaaccgc aaagcattat cgcgaaggat
     5401 agcgccgctt tgccctggct gcaggtgttg ctccagaccg atccgggcaa tgtctacgcg
     5461 cagtatatgc tagggcgagt tgattatgac ttgcaggaat atgccgcaag caaagccagg
     5521 ctgcttgctt cgctacaact cgtctcagat gaggcgtatc gctcatctgt ctacacctat
     5581 gttgccttca gcgatgatcg attgggcaat tatgtagagg gacgaagcat gttactggaa
     5641 gcagtacagc tcgacccgga gtataggaac gatgttgcgc gtggagccat ctctggtttg
     5701 tagtgagcgg cgctagaagg acaaacagat gatctccacc catacaacga cgcaggcgat
     5761 accgcagaca ccggaggtat atgttgcgga aacgcgcgcc acagaaaccc ggcgcgcgcg
     5821 tcccttcaag gcgctggcag ggctggtgtt agcactggcc tgcctgggcg caagctggtt
     5881 cgcgtaccag tctgagctgg cgccacagcc aaaagatttc cagcccgcct ggcatgatgc
     5941 gcagtggatt ggagcatcgg acggcaacgc cacgaccgcg tattatcgct atgccaccac
     6001 attagaggta gcgacggata acgcattcgt cacaattgcg gccagccagg tcttcagttt
     6061 ctatgtcaat ggcgtcttga tcggctcgaa tagcacagat tttgccaatt atccccgctc
     6121 ctatatctat gaggtcacgc catcacttgt tctgggcgtc aacgtttttg caattcgggt
     6181 tgataacctg gatggacagg ctcctctgct gcgtgccagt ttcggcactg ttatcggagg
     6241 gatcgttacg tactatggaa ctggcgacgg cgcaggtgca aactggatgg cgacgacgca
     6301 gaccacgcag gtctacccgc aagacacatc gattatcgtc gccgctaccg cctggaccgg
     6361 gaggaccttt aacgccgctt cctggctgcc cgctcagccg gtgacatcat ctacgcagtc
     6421 catgctcacg tcgcaatcgc cctcgctcag tgtcaatcca cagttgtacg agcagccggt
     6481 cgccgcgcac tgggtcagca cgggggttgg gcaggatggc tactttgtgc gccaggtcag
     6541 cttgccgcag ggtacgagca tgagctggct gcgtatcgcg gcgaccggaa cggcgaatgt
     6601 ctttatcaat ggcagcctga tctttacctg gaactaccat cgcacttctt tctcgcagat
     6661 caccgtcaat aagttaaccg gcaagacaaa ggtgcggcat atccctgaac tggtgctggg
     6721 cgtctatgat gtctccccat ttcttcatgc gggggtaaat accgtcgcga tccatatctc
     6781 gctccagaat gtgccagggc tgcgcaatat tcaggtgacg gaagcgcccg cgctctcgat
     6841 ggatgtgctt gcgggtgacg gccagggcga ttttacgtgg attggctctc ctgatggatg
     6901 gcaggcatca caccagcaag cgcccgcctg gataaacggc ggcgcggcgg tacatgactg
     6961 gacttctcct actttcacgg ggcggcccga tggctacagc atcgtctatg tgtccaggag
     7021 cagcgaactg accaggggac agcaggcagt gcaaagcgtg ccggttgaac aggccatcat
     7081 ggtgatactc ttgagcatcg cggctgtgct gggcctgtgg ctgctggtat cgctgacgat
     7141 cgcgcgccga tttgttcact cagcttcgac tgcactggaa ttgatgagcc tggcctacct
     7201 gcccgcgctg gccggcgagg gtcttttgat cgtgatttcg aaggagccgc aggttgcgca
     7261 gcccttcccg tataccacgc tctggggcct gatgctgctt gctggggtag cggcgggcta
     7321 ccttttgatt agcctctccg ttggattgag gcgagcgacg gggcgaccac atgacgagcg
     7381 tgatgcgttg gaagggcgac cacaagggta cgcccccata ggcgttaact taacgaccaa
     7441 gtctgtcatc ctgagcgcag cgaaggatct gacgcgccgg ggaccgagat ccttcgctgc
     7501 gctcaggatg acattcgacg acccgtcgcg caacacgatc gctcaggaag gtcagcttaa
     7561 gttaacgctt atggggtacg cccctcgtgt gcagcgaccg ctcgactggc tcaagcgtca
     7621 ctggccaata gtcgtgctgc ttgtggcgac gatgccgctt gtgttttatg acctgggata
     7681 cgaggcgtac tggcaggatg aactggtcag ttacctggtg gcgaaatcga ttctgtataa
     7741 tggcctgccg atgctgccat ccgggtttct ctacccgaaa ggcgaactgt attcatatct
     7801 cctggcgctc tttatgagca tctttggcga tcagggtggc gggcctcgcg tgattagcgg
     7861 cgttgagtat ctgctgacgc tggcgctggt gtactacgtg ggctgtcgct tcttcgaccg
     7921 gcgcgtcgcg ctggttgccg cgctcatgct ggcgttctct ccccacgagt tgatctgggc
     7981 gcggcagatg cgcatgtatc agcaggcgca gttgctgacg ctgctgacta cctatctgtt
     8041 ttaccgggcc gttcaggagc gtcatcgtgt gtcgctcgtc tacctggcaa caggtgtgct
     8101 gctcgtcacc tatctgagcc acgaggaaac ctttattatc ttgcctgccc tcgtcctgtg
     8161 cctgctggtg gtggggtata tcgtgaagga ggagcgacac cggctcccct ggtttttcta
     8221 tcagaaacac tggtggatcg ccgcgagcat cgcggtcggg gtgattggga cgcaactgct
     8281 catcacgaag gtcacgcatc ctcccgtgct gggaaccgac accactgtgc gaccgatgat
     8341 ccagttgacg accaataaca tcccctatta cctcaatatg ctctttacgc cgacctcgaa
     8401 tgacccgtcg aacagtacgc tggtctcgaa tatcccgtcg tcgatcatgc tggattcctg
     8461 cctgatgctg ctgggctgca tcctggccat gaaaagcaat cgactggcgc tgcgctttac
     8521 cgccctgttt ttcgtggtaa gcttcctgac gctggtattt gtcctcacca tgcaggctga
     8581 tcgctacttt tacccgctcg ccccttttta ctacttacta gcggcgtttg cgttcgtcaa
     8641 gatcgcgcgc ctggtctggg tgtttgccaa aactcgatta gcgtctccta acagtcgccc
     8701 ttccgcatct atcatcaagt ggcttgtggg actgtcggca gcgctggcgc tggtcagtgt
     8761 gctactggcg ccaatgttcc cgttgaataa ctacaacctg tacgtgagcc gcttgttcaa
     8821 tctgccttac tatcatcatt ttggggatta tgacgaggct gggctatata tccggcagca
     8881 tatgcggcag ggggatatcg tcatttcaat tattcccgac tctattgtga tgttttatgc
     8941 cggccaatcc gattattttt tctcggtcaa tcacgcgctg tttctctttg agcaggatag
     9001 ccacattgtt gatacatata gtgggaaggt tgccctattg cgtcaatcag accttgatgc
     9061 tgtgctggat atgcacgcgc gcgtctggct gttttcggcg aataaccact atcagaacga
     9121 ggtgctgaaa cactttattt tgccgtccga ttttcatgtt gtgtattcag gggctgactc
     9181 gattgtgt
//
LOCUS       DASZAK010000158         1993 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_465432, whole genome shotgun sequence.
ACCESSION   DASZAK010000158 DASZAK010000000
VERSION     DASZAK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465432"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1918)
                     /locus_tag="VGT44_02715"
     CDS             complement(<1..1918)
                     /locus_tag="VGT44_02715"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEV2579740.1"
                     /translation="MITIDEQQVLLHTSATSKTEAIRQVGQLLVESGCIEAGYVDSML
                     GREQVANTYLGNGIAIPHGLPADRDLIKRTGVAVVQAPYGVTWNPGETAQLIVGIAAK
                     SDEHIEVLRRLTRVLGDMAQVNRLTQTNDSRDIIEALTGERPAPPAKHEPTITDYPTY
                     FDAIIQNKTGLHARPAAFFVDLAKRFQADIKVRHESETASGKSLIALLQLGVTPGSTI
                     RVSAQGADASAALDALRSAIASGLGDEMEEQQPNQPTSPTQTWTPQAPGITLSGVSAS
                     GGLAIGVTRQFKPRVITVEDMPGDALTEGERFQEALNAAQAELNQLYEEVKTRLGSGK
                     AAIFRVHAELLNDAEIIQQTIVSIFQRHSAAWSWQQVINERVNQVQKLDDPVLAGRAV
                     DLSDVGQRVLRQLVGMSDAQVVAPETPVILLAEDLTPSDTAALDPDSILGFCTVKGGP
                     TSHTAILARSMGIPAIVGAGATILDIANGTPGILDGTAGKLYLKPGEADVQAARALQQ
                     RLQHRQDEANTTRFAPATTSDGYRIEVAANINRAADAPPAVEAGAEGVGLMRTEFLFL
                     ERTSIPTEEEQFEEYRDMALALGGRPLIIRTLDIGGDKEVPYLNLPKEDNSFLGIRGV
                     RFTLARPDLFIPQLR"
BASE COUNT          348 a          588 c          619 g          438 t
ORIGIN      
        1 cgcgcagttg cgggatgaac aggtctggcc gcgccagcgt gaagcggacg ccgcgaatgc
       61 ccaggaagga attgtcctct ttgggcaggt tgagataggg tacctctttg tcgccaccga
      121 tatcgagcgt gcgaataatc aacgggcgac cgcccaacgc cagcgccatg tcacggtact
      181 cctcgaattg ttcctcctct gttggtatgg aagtgcgttc gaggaagaga aactctgtgc
      241 gcatcaggcc cacgccttcc gcgcctgctt cgaccgccgg gggcgcgtcc gcggcgcggt
      301 tgatgttggc ggctacttcg atgcgatagc cgtcgctggt ggtggccgga gcgaaacggg
      361 tagtgttggc ctcgtcctgc cgatgctgca agcgctgctg caaggcgcgc gctgcctgca
      421 catcggcctc acccggcttc aagtacaact tgcctgctgt gccgtccagg atacccggcg
      481 taccgttggc aatgtccaga atggttgcgc cagctccgac aatggcggga atgcccatcg
      541 agcgggcgag aattgccgtg tgcgaggttg gcccgccctt gaccgtgcag aatcccagga
      601 tgctgtcggg gtcgagcgcg gcggtatcgg atggggtcag gtcttccgcc agcaggatca
      661 ccggcgtctc cggcgcgacg acctgcgcgt ctgacatccc gaccagttgt cgcagcacgc
      721 gctgtcccac atcgcttaaa tcaacagcgc gcccggccag caccggatcg tcgagtttct
      781 gcacctggtt gacccgctcg ttgatgacct gctgccagga ccaggcggcg ctgtggcgct
      841 ggaagatcga gacaatggtc tgctggatga tctctgcgtc gttcaacagt tcggcatgaa
      901 cgcggaaaat ggctgcctta ccggagccga ggcgcgtctt gacctcttca tagagctggt
      961 tcagctcggc ctgcgccgcg ttgagcgctt cctggaaacg ctcgccctca gtcagggcat
     1021 cgcccggcat atcttcaacg gtgatgacac gtggcttgaa ctggcgtgtc acgccgatgg
     1081 ccaggccacc ggacgcgctt acgccgctca gcgttatgcc gggcgcttgc ggcgtccagg
     1141 tctgtgtggg ggaagtgggt tggttcggct gctgttcttc catctcgtcg cccaatccgc
     1201 tcgcgattgc ggaacgcagc gcatccaggg cggcagacgc gtcagcgcct tgcgccgaga
     1261 cgcgaatagt tgaaccaggt gtgacgccaa gctgcaataa cgcaatcagg cttttgccac
     1321 tggctgtttc tgactcgtgc cgcaccttga tatcggcctg aaagcgctta gccaggtcta
     1381 caaagaaggc ggcaggccgc gcgtgcaggc cagtcttgtt ttggatgatt gcatcaaaat
     1441 aggtgggata atctgtgatg gtgggttcgt gttttgctgg cggggcaggc cgctcgcctg
     1501 tcaatgcctc gataatgtca cgcgagtcgt tcgtctgtgt caggcgattg acctgcgcca
     1561 tatcgcccag tacgcgcgtg agccgccgca gtacctcgat atgctcgtcg gattttgcgg
     1621 caatgccgac aatcaattgt gcggtttcac cagggttcca ggtcacgcca tacggggcct
     1681 gcacaaccgc tacgccagtg cgcttgatca ggtcgcggtc ggcgggtaat ccatgcggta
     1741 tagcgatgcc gttgccgagg taggtgttgg cgacctgttc gcgcccaagc atactgtcga
     1801 cgtatcctgc ctcgatacat ccactctcga ccagcagttg ccctacctgc cggatagctt
     1861 ccgtcttact ggttgcactg gtgtgtaaca gcacttgctg ttcatcaatc gtaatcatcg
     1921 tcgtctccag cgtcgttgca atgtgaagcg ttacgatctc gttgttcctg atgtgaaaat
     1981 tgtaatcgat tac
//
LOCUS       DASZAK010000159         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_678669, whole genome shotgun sequence.
ACCESSION   DASZAK010000159 DASZAK010000000
VERSION     DASZAK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678669"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VGT44_02720"
     CDS             complement(<1..543)
                     /locus_tag="VGT44_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEV2579741.1"
                     /translation="MTYHKTGTREEWLAARLELLEAEKALTRRGDELARRRRELPWVR
                     IDKQYRFETDEGAASLADLFRGRSQLLIYHFMFGPDYTAGCLSCSAIADGFNGSVVHL
                     ANHDVTLCAVSRAPLAKLQAYKQRMGWSFPWASSFGSDFNYDFQAAMTEKQQQSGAVE
                     YNFRVTDLRLEPEKDKESQSG"
     gene            complement(642..1025)
                     /locus_tag="VGT44_02725"
     CDS             complement(642..1025)
                     /locus_tag="VGT44_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2579742.1"
                     /translation="MIDSHAQRVFLALADPTRRLLLQQLCQEGSGTAASFATRLPITR
                     QAITKHLVTLEQAGLVTVHEMGRERRYVPRPEALSTVTTWIGAIEAQWDQRLAALRSY
                     LLEEPSTPSVQEKEPHAGASSPQDE"
     gene            complement(1111..1575)
                     /locus_tag="VGT44_02730"
     CDS             complement(1111..1575)
                     /locus_tag="VGT44_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV2579743.1"
                     /translation="MTLSDRIERTMVLPAPRQRVWEAITKPEQLAHWFGVVSDMDFRV
                     GGAIHFTWENEPCPYTGRIEVIEEPHRFAFRWPSYAVGHPKVTFATAPSTLVEITLED
                     RAEGTQLTLVESGFASLPQPIPAGEAYQDNQQGWQACLSALHAYLQSQVEAV"
BASE COUNT          461 a          646 c          616 g          495 t
ORIGIN      
        1 tccactctgg gactctttgt ctttctcagg ttcaagcctc agatcagtga cacggaagtt
       61 gtactcgacc gctcctgact gttgctgctt ttcagtcatc gccgcttgga agtcgtagtt
      121 gaagtcgctg ccgaacgacg acgcccacgg gaagctccag cccatccgct gcttgtaggc
      181 ctgcagtttc gcgagcggag cccgcgacac cgcgcagagg gtgacatcgt gattggcaag
      241 gtggacgacg gagccgttga agccgtccgc gatcgccgag cagcttagac accctgccgt
      301 ataatcgggt ccgaacatga agtgatagat gagcagctgc gagcgtcctc tgaagagatc
      361 ggcgagggac gctgcaccct cgtcggtctc gaagcggtac tgcttgtcga tgcgaaccca
      421 gggcagctcc cgccgccgcc gcgccagttc gtcgccgcgc cgcgtcagcg ccttctcggc
      481 ctccagcagc tccagccggg ccgcaagcca ctcttcacgt gttccggtct tatgataggt
      541 catggtttct ctccttcttg ttggtcgatc tgatcatgtt tatgcaatga atgtttgagt
      601 gccactctcc cttgccactg tctaaccgtg gatgcgaggg actactcgtc ctgaggggag
      661 ctcgccccgg catgaggttc tttctcctgc actgatgggg tgctcggttc ctcaagcaga
      721 tagctccgca gggcagccag acgctgatcc cattgtgctt caatggcccc aatccaggtt
      781 gtgacggtcg agagcgcctc tggtcgtggc acataccgcc gctcccgacc catctcgtgg
      841 accgtcacga gaccggcctg ctctaaggtc accagatgct tggtgatcgc ttgacgcgtg
      901 atgggcaaac gggttgcgaa gctcgcggct gtccctgagc cttcttggca caattgttgc
      961 agcaacagac gacgcgtggg atcagccaga gccagaaaga ccctctgcgc gtgtgaatcg
     1021 atcattaccg gcttctcctc tcctgtgggc gacatgctga tcacgcatta cccacaacca
     1081 gcaagcccct gaccaggacc gcgacagcag ctatacggct tccacctggc tctgcaggta
     1141 ggcgtgcaac gcggaaaggc acgcttgcca gccctgctgg ttgtcctggt aggcttcccc
     1201 tgccggaata ggctggggca gggatgcaaa acccgactcg accagcgtca attgggtccc
     1261 ttcggcccgg tcttccaggg taatttccac cagcgtactg ggggctgtgg caaacgtgac
     1321 cttggggtga ccaaccgcat aggaaggcca gcgaaaggca aaacgatgcg gctcctcaat
     1381 cacctcgatc cggcccgtgt aggggcaagg ttcattttcc caggtaaaat ggatggcacc
     1441 gcccacgcga aaatccatgt cactcaccac tccaaaccag tgggcaagct gttctggttt
     1501 cgtgatggcc tcccagacac gttggcgcgg ggcgggcaag accatggttc gttcgatacg
     1561 atcagaaaga gtcattggat ctcctctcca gtgaagcaag atgctcttgt gtgttgctct
     1621 ataggcaact ctcaggttgc ctatagagag tataccagaa aaaatcacaa taagcaaccc
     1681 caaagttgcg tgttttgcac atggggtcag acgcgcgcgc aaaatgggag acgtggtggg
     1741 cgtggaccgt tggatgcggc tctcttctgc catggggtca cgagattatc cggggcaaaa
     1801 cgcacgttaa gccatgaaac ctcttccctg gcgccgtctc ttatgaggca aaaggaagaa
     1861 tggcacgctt gcggcgtctc ataaatggcc gtttcatgcg tctcaaatcc gaccgtttct
     1921 tgcgacaacg agactcccgt caggactggg ttctggcgtc tcaattcgtg tccatgataa
     1981 cctgatgtgc ttgtgccaaa acttgtctca aaatcaaaat ctcatgttct tcgatacgag
     2041 atgagtaagt gagacaaaat gtagagctta tttgtatcta tcagtcatcg tcactgccag
     2101 gatatgtaaa ccatccgtgg cgaggtttgc ctgctaacca ctgcccgccg gtttctgtac
     2161 agtagtgacc atttccactt ggacaccagc atgtattgac cgcccagctc cacgggaa
//
LOCUS       DASZAK010000160         2800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_501088, whole genome shotgun sequence.
ACCESSION   DASZAK010000160 DASZAK010000000
VERSION     DASZAK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501088"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1711)
                     /locus_tag="VGT44_02735"
     CDS             complement(<1..1711)
                     /locus_tag="VGT44_02735"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2579744.1"
                     /translation="MAKMTPTVREDQLVYQHGEHEQMLVVETPAWYAWLETASTFAFT
                     SEAGTFTARKERAGNQRGGWYWKAYRMQQGKLSSRYLGKSETLSLHRLQAAAQALASH
                     SDPDRKAALPATLTQGDPLLATKLHVPRPRRQLVSRPALIKRVKEGIQGPLTLISAPA
                     GFGKTTLLAQWAAESGMPVAWVSLEPQDNDPVRFLASVLAALQQLDPHMGTTALALLH
                     SPQPVPPERVLAVLTDELTNREEGGFALVLDDYHAISAAPIHGSLTFLLEHLPPQMRL
                     VLATRADPPLPLARLRARGQLSEVRAAELRFTTEEARTFLHMVMGLSLAPEAIAALES
                     RTEGWIAGLQLAALSLRERADVSSFLTAFSGSHRFVLDYLSEEVLARQGAQVQSFLLT
                     TSILSRLSGPLCDAVREQEGSQAMLERLEQANLFVVALDDGRHWYRYHQLFAEVLQSR
                     LQQTEPTLAPELHRRASRWYEDHALFVEAVQHALAASDVERAAHLLEQHWPSLASQGY
                     IQTLLSWLKMLPETLMQTRPRLCVVRATVLLITNQLDEVPARLQQAEQALQAHDVGIS
                     VEEG"
BASE COUNT          592 a          825 c          866 g          517 t
ORIGIN      
        1 gtccttcctc gacagagatg cccacatcat gcgcctgcag cgcctgttct gcctgctgca
       61 ggcgtgccgg gacctcatcc aattgattgg taattaacag caccgtcgcc cgcacgacgc
      121 agaggcgggg acgcgtctgc attagcgttt caggcagcat cttgagccag ctcagcagtg
      181 tctggatata gccttgactg gcgagcgagg gccagtgttg ctcaagcagg tgggcagccc
      241 gctcgacatc agaagcggcg agcgcatgct gcactgcttc gacgaagagg gcatgatcct
      301 cgtaccagcg actggcgcga cgatgcagtt cgggggccag ggtaggctct gtctgctgca
      361 gacgactctg cagcacctcg gcaaagagct gatggtagcg gtaccagtgc cgcccgtcgt
      421 cgagcgcgac gacaaacagg ttcgcctgct cgagccgctc cagcatggcc tggctccctt
      481 cttgctcgcg tacagcatcg caaagtgggc cagagaggcg cgagagtatc gaggtggtga
      541 gcagaaacga ttgcacctgc gcgccctgtc gcgcgagcac ctcctcggag aggtagtcca
      601 acacaaagcg atggctgccg ctgaaggcgg tgagaaagct agagacgtcg gctcgctccc
      661 gtagggagag cgcggccagc tgcagtccgg caatccagcc ttccgtacgg ctctccaagg
      721 cagcaatagc ctcgggagca agagagagcc ccatcaccat gtgcagaaag gtgcgggcct
      781 cctccgtagt gaagcgcaac tcggccgcac gcacctcgct gagttgccca cgggctcgca
      841 gacgggccag cggcaggggc gggtcggcgc gcgtggcaag taccaggcgc atctggggcg
      901 gcagatgctc caggaggaag gtcaggctcc catgaatggg cgcagccgaa atcgcgtgat
      961 agtcatccag gacgagggcg aagcctcctt cctcacggtt cgtcagctcg tcggtcagga
     1021 cggcaaggac tctctccggc ggcacgggct gcggggagtg aagcagcgcg agggcggtcg
     1081 tccccatatg cgggtcaagc tgctgcaggg cggcaagcac cgaggcaaga aaacggacag
     1141 ggtcgttgtc ctgcggctct aacgacaccc aggccacggg catgccgctc tcggccgccc
     1201 attgggccag cagcgtcgtt ttcccaaagc ccgcgggagc cgagatgaga gtgagcggac
     1261 cctgtatgcc ttctttgacg cgcttgatca aggcggggcg agagacgagc tgccggcggg
     1321 gacgcggcac atgcaatttg gtcgccagta gcgggtcgcc ctgcgtaagt gtcgcaggga
     1381 gggccgcctt tcggtcaggg tcagagtgac tggccagcgc ctgagccgcc gcctgcaagc
     1441 ggtggagcga gagcgtctcc gacttgccga ggtagcggga ggagagcttg ccctgctgca
     1501 tgcgataggc tttccagtac cagccaccac gctgattgcc ggcgcgctct ttgcgggccg
     1561 tgaaggtgcc cgcctcactc gtaaaggcaa aggtggaggc ggtctccagc caggcatacc
     1621 aggcgggtgt ctccacgacg agcatctgct cgtgctcgcc gtgctggtag accaactggt
     1681 cctctcgtac cgtgggtgtc atcttggcca tggctgtgct ctcctataac aggctctgcc
     1741 cgaaaccttt ccttaccgaa caccagtata gcataggttc ggtaattcgt gaccggagcc
     1801 gaacgtctgc cctaacggat gcccgggcag gaacactgcc cggagaacgg tctcatgttc
     1861 tcagcaggtg cagagcaagt actgcaccat gtatccgacg tggtcagcgc tccgaaaaaa
     1921 gtgagcgatt ctcggtctac gggctccatg ggacatccgt tcctccaaca agagcacagg
     1981 ggtgtgactg aggagtaggc aagaggcgct cttgtcggga gggatgctct tcgagcgtgt
     2041 tgtcagtggc agcctcaact cgcgcaagca gcacgacttc ctcctcaccc tgttccgccg
     2101 tgaatgtgtg gcgtacagcc ttcttccttg agagcaaggg aagattcaaa agatacgccc
     2161 aagacccgat cggagtcttg ggcatgatcg cggaaccgac cggaaccgat gggtcgatgc
     2221 ccatcggtcg cccctggcac gtattcccct tgaaaagctt ttctccagta aaggcgatct
     2281 ccttctctac caggaacacg ctgccacttc ctcatcccaa gatgggagtc acccgctgtt
     2341 ctggagaccc gaaatagtag ggcgaaccca tcatgtgtgc cgaagcaacc gggctgtggc
     2401 gcccagcgca gagggctacg gaaacggagg acagatcagt caaacatcac taaggggctt
     2461 acggtgagaa gcagaggccg aggtcttaga acctttgttc tattatatct agaacaattg
     2521 tataaatagt agagcggaac cgacgggcta cgactacgct gtcgccaatt ttatacctct
     2581 tccaattacc ctcatctacc ctgtaccgca agctccaaaa agtactgtca gagacagttg
     2641 tagagccagt tgttgaacag cgcagacctg gcccaagaga cattccctat ggtattccag
     2701 cggacgaatg gcatattgtc cttgcaagag ttgctaacca tgagtcctac cgcacaatcg
     2761 cgaaagacta tggtgtttca agagaaacca tacgccgtct
//
LOCUS       DASZAK010000161         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_145871, whole genome shotgun sequence.
ACCESSION   DASZAK010000161 DASZAK010000000
VERSION     DASZAK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145871"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1126)
                     /locus_tag="VGT44_02740"
     CDS             complement(<1..1126)
                     /locus_tag="VGT44_02740"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HEV2579745.1"
                     /translation="MNVDAITLAAVADEWSTLLAGARIDTIIAPTANAIAVQCYAPGG
                     AGGQEPEAGRSGANRWLYLSAHPQLARVHLTANKPARMVSEPSAFVMLLRKYLEGTRI
                     ESIAQPRWERVIELVASHRAGPDSDERVRYRLIVEVMGRISNIIFCDEQERILGALKH
                     VGADINRYRVIAPNVPYVPPPPQHRSIGGLSLPRLDPASVTAEALHQAALNEIAAFAL
                     PPMAVPAKSSRKARQAELPLLWQLLTKHLLGFSQLIAREAVYRATGGIDAPLTQEAAT
                     WEELAWNVRELAALLDTHAWRPQLIERVASGEASKAAGMEGASPGLLAFAPYVLEQYA
                     ETPDVRARAMLSINAAIDDYYAGAEWRAAMESVRAPLRRVL"
     gene            1250..1900
                     /gene="gmk"
                     /locus_tag="VGT44_02745"
     CDS             1250..1900
                     /gene="gmk"
                     /locus_tag="VGT44_02745"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV2579746.1"
                     /translation="MQLETEALVSQPAWTPPSTSRKGLLFVLSAPSGAGKDSVINAAL
                     EQGMNFYVVPSVTTRARRPGESEGHPYNFVSQEQFEQMVAHDELIEYAKVHGNWYGQP
                     RKPIRERLSAGQDVLLKIDVQGAASVRKKIPGAIFIFLVPESLEVLASRLIHRNTETA
                     EELAQRLENARAELAEQHRYDYVIVNRQEHLAEAAEQLWAIMEAEHCRVHPRHVEI"
     gene            1922..2935
                     /gene="glpX"
                     /locus_tag="VGT44_02750"
     CDS             1922..2935
                     /gene="glpX"
                     /locus_tag="VGT44_02750"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEV2579747.1"
                     /translation="MAEDRFMPSGTRERNLGMELVRVTEAAALSAARFMGRGDKIKVD
                     QAAVDAMRQAVDGVDMHGVVVIGEGEKDEAPMLYVGEEIGNRTPPRVDVAVDPVDGTR
                     LTALGLPGAIAVVATAERGSMYSAPPGVYYMDKIAVGPAARDVIDINAPVAENLAHIA
                     KAKNLRIDDLIVVILDRPRHEDIIRQVREAGARIRLIMDGDVSAAIQAAMEDYKGVDV
                     LLGMGGAPEAVLAAAAIKCIGGAIQCKIWARSDEEREKLKAVGIDFEKIYTTDDLVRG
                     EDISFAATGITSGELVDGVQFFGWGARTSSIMMRSRSGTIRYIQARHKWRSKAPNGKS
                     AAD"
     gene            complement(2932..3075)
                     /locus_tag="VGT44_02755"
     CDS             complement(2932..3075)
                     /locus_tag="VGT44_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579748.1"
                     /translation="MERGIAQVIAEGKYVTYDLKPRPDDPTAVGTSQVADAVIEKMKH
                     SAV"
BASE COUNT          631 a          955 c         1026 g          576 t
ORIGIN      
        1 gcaacacgcg gcgcagcggt gcgcgtacac tctccatcgc ggcgcgccat tccgcgcccg
       61 cgtaataatc gtcgatggcg gcgttgatgg acagcatggc gcgggcgcgc acatcgggcg
      121 tttccgcgta ttgttccagc acatacggag cgaaggccag caggcccggc gacgcgccct
      181 ccatccctgc tgcctttgac gcctcccctg acgcgacgcg ttcgatcaac tgcggtcgcc
      241 aggcatgggt atcgagcagg gctgccagct cgcgcacatt ccaggccagc tcttcccagg
      301 tcgccgcttc ctgcgtcagc ggcgcgtcaa tgccgccggt ggcacggtag acggcctcgc
      361 gcgcgatgag ctggctaaag ccgagcaggt gtttcgtcaa gagctgccag agtagaggaa
      421 gctccgcctg acgcgccttg cgggatgatt tcgcgggaac ggccatcggg ggcagcgcga
      481 aggcggctat ttcgttcagc gcggcctggt gcagcgcttc agccgtgacg ctggccgggt
      541 ccaggcgcgg cagcgataga ccgccgatgc tgcgatgctg cggcgggggc ggcacgtaag
      601 gcacgttagg cgcgatgacg cgatagcggt tgatatcggc gccaacatgc ttgagcgcgc
      661 cgagaatgcg ctcctgctcg tcacagaaaa tgatattgct gatgcgcccc atcacctcga
      721 cgatcagacg atagcgcacc cgctcatcgc tgtctggccc ggcgcggtgg ctcgcgacaa
      781 gttcgatgac ccgctcccag cgcggctgcg cgatggattc gatgcgcgtc ccctccaggt
      841 atttgcgcag cagcatcacg aaggccgaag gctcgctcac catcctggca ggtttgttcg
      901 cggtcaggtg gacgcgcgcc agctggggat gcgccgaaag gtagagccag cgattcgccc
      961 cactccgtcc ggcctccggc tcttgtcccc cagcccctcc tggggcgtaa cactgcacgg
     1021 cgatggcatt cgccgtcggc gcgatgatag tatcgatcct cgcgccggcg agcagggtgc
     1081 tccattcatc ggcaacggcg gccagggtga ttgcatcaac attcattcgt ttatccgttc
     1141 aagtggcata gacccatttg gagtgttgta caataatgac aataagagtt atcccattga
     1201 cgtagtgtaa caaagagtgg ggattttgta tatcctcaga ggtaaaatta tgcagcttga
     1261 gacggaggcg ctcgtgtcgc agccagcatg gacaccgcca tcgacctccc gcaagggact
     1321 cttatttgtg ttgtcggctc catctggtgc agggaaagac agcgtcatca acgccgcgct
     1381 cgagcaaggc atgaatttct acgtggttcc ctcggtcacg acacgcgctc gccgaccagg
     1441 agaaagcgag gggcatccct acaatttcgt cagccaggag caattcgagc agatggtcgc
     1501 tcacgatgag ctgatagagt acgcgaaagt gcatggcaac tggtatgggc agccgcgcaa
     1561 gcccatccgc gagcgcctga gcgcagggca ggatgtgctg ttgaagatcg atgtgcaggg
     1621 cgcggcgtct gtgcggaaaa agatacctgg ggccatcttc atcttcctcg ttcccgaatc
     1681 gctcgaagtg ctggccagcc gcctgattca ccgcaatacc gagacggcgg aggaactcgc
     1741 gcagcggtta gagaacgcgc gcgcagaact ggccgaacaa caccgctacg attatgttat
     1801 cgtgaaccgg caggaacacc tggcagaggc cgcggagcaa ctgtgggcca ttatggaggc
     1861 ggaacactgc cgtgttcatc cccggcacgt cgaaatatag tcaggctaag gagggcattt
     1921 catggctgaa gatcgtttta tgccatcggg tacgcgcgaa cgcaacctgg gcatggagct
     1981 ggtgcgcgtg acggaggcgg cggcgctgag cgcggcgcgt tttatgggga gaggcgacaa
     2041 gatcaaagtt gaccaggccg ccgtcgatgc catgcgccag gccgtagacg gcgtagacat
     2101 gcatggcgtg gtcgtgatcg gcgagggcga aaaagacgaa gcgcccatgc tctatgtcgg
     2161 cgaagagata ggcaatcgca cgccgccgcg cgtcgatgtt gccgtcgatc cggtggatgg
     2221 cacacgcctg acggcgcttg gcctgccggg ggccatcgcc gtggtcgcga cggcggaacg
     2281 cggctcgatg tactccgcgc cgccgggcgt ctactacatg gacaagatag ccgtcggacc
     2341 tgccgcgcgc gacgtgatcg acatcaatgc cccggtggcc gaaaacctgg cgcacatcgc
     2401 aaaggcgaaa aacctgcgca tcgatgatct gatcgtcgtc atcctggatc gcccgcgtca
     2461 cgaagacatt atccggcagg tgcgcgaggc cggcgcgcga atccggctga ttatggacgg
     2521 cgacgtatca gcggcgatcc aggcggccat ggaggactac aaaggcgtgg atgtgctgct
     2581 cggcatgggc ggcgcgcccg aagcggtgct ggcggcagcg gccatcaaat gcatcggcgg
     2641 ggccattcaa tgcaaaatct gggcgcgttc ggacgaggaa cgagaaaagc taaaagctgt
     2701 cggtatcgac ttcgagaaaa tctatacgac cgacgacctg gtgcgtggtg aagacatctc
     2761 ctttgccgca acgggcatca ccagcggcga actggtcgat ggcgtacagt tctttggctg
     2821 gggcgcgcgc acctcgtcga tcatgatgcg ctcgcgctcc ggcacgatcc gctacatcca
     2881 ggcgcgccac aagtggcgca gcaaagcccc caacggcaag agcgcagctg attaaacagc
     2941 tgagtgtttc atcttctcaa tcaccgcgtc ggcgacctgc gaggttccca ccgctgtcgg
     3001 gtcgtcggga cgcggcttga gatcgtaggt cacgtatttc ccctcggcaa tcacctgggc
     3061 gatgccgcgt tccatgcgat tcgccgcgtc ctacacgccc aggtggcgca gcatcatgat
     3121 gccggagaac atcatcgcca acggattgac cttgttctgt ccggcatatt tgggagcgct
     3181 gccatgca
//
LOCUS       DASZAK010000162         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_572025, whole genome shotgun sequence.
ACCESSION   DASZAK010000162 DASZAK010000000
VERSION     DASZAK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572025"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VGT44_02760"
     CDS             complement(<1..972)
                     /locus_tag="VGT44_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HEV2579749.1"
                     /translation="MAHDFHIAESRLHALVSSFASKRVLVIGDMVADEYLIGRPTRIA
                     REAPVLILELDDERTIPGGAANVAVNARTLGAEVFLAGGVGDDVPGKRLRRAVAELGM
                     RQEGLVTDAQRPTSTKTRILAGSPQIVQQHIVRIDRVDTSEVGQPAKGNIREYIQHML
                     PSIDAMILSDYENGVISPEIIETCIPMARELHKVVVVDSHGSLFRFQGVTALTPNQPE
                     AELTLGMTITSQAELEEAGQRLLDGSHAMSVLITRGSEGMSLFEAGKSPMHLPVHRLD
                     HGIEVVDTNGAGDTVAATFTLALATGASCAEAAYLANAAAALVVRRLG"
     gene            complement(962..1738)
                     /locus_tag="VGT44_02765"
     CDS             complement(962..1738)
                     /locus_tag="VGT44_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV2579750.1"
                     /translation="MSQQSPPAPAYVLGVRVDRLSQRQALDMIEQLIASRRTNDNALP
                     CQQIVTVNTEFVMAAQQNTDFRQAINSAALVVADGIGVVWATHFVHAPAPERITGTDT
                     LVALAKRCAEKGYRLYLLGAAPGVAELAGVCLQTLAPGLQIAGTYAGSPAPAEEDAIL
                     ERIRTANADVLCVAYGAPAQDLWIHRNLSRLPVAVAMGVGGAYDFLSGRQQRAPRAMQ
                     RLGLEWLYRLYREPWRWQRMLAIPRFIIQVLLKGRKKHGP"
     gene            complement(1770..2048)
                     /locus_tag="VGT44_02770"
     CDS             complement(1770..2048)
                     /locus_tag="VGT44_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEV2579751.1"
                     /translation="MSQITLHLPECHSLKDKRQVIKSVMARVRQQFEVAIAEVDEQDR
                     WQIAKLGVSCVSNSSQHIDEILGRVRRYIEETRPDVIVTDAEAEIINW"
     gene            2239..2952
                     /gene="upp"
                     /locus_tag="VGT44_02775"
     CDS             2239..2952
                     /gene="upp"
                     /locus_tag="VGT44_02775"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV2579752.1"
                     /translation="MTETTTSSGGRPSLPMQGIPQPRRDEVLDLPRVHISSHPVMAHK
                     MTALRDKNTQPPEFYRLVKEIGALLAYEATATLALETVPIETPMGPMVGERLAGGIGV
                     TPILRAGLGLAEGFREVIPDAQIWHLGLRRDEETLQAIAYYNQLPHQVDLQVCYAVDP
                     MLATGGSAIDAINVLKRRDIPRLCYVGIIAAPYGLLKLSQTHPDIDIYIAALDESLND
                     VGYILPGLGDAGDRQFGTF"
     gene            2983..>3327
                     /locus_tag="VGT44_02780"
     CDS             2983..>3327
                     /locus_tag="VGT44_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579753.1"
                     /translation="MAFPFSPRNEGDDEEVTDSTDEGLEFPSGDQVTAEEQPSAEETP
                     ATQLPPEAQGEVNGGPLGCCLGVTIGLLLSLTVAILSRLYADPLAQIFHSSLSITVRI
                     VMSLLAIACAVLF"
BASE COUNT          760 a          936 c          876 g          755 t
ORIGIN      
        1 tcccagtcga cgcacaacga gcgcagccgc tgcgttggca aggtacgcgg cttccgcaca
       61 gcttgcgccg gtcgcgagag ccagtgtaaa ggtcgcagcg accgtatcac ccgcgccatt
      121 cgtatcaacg acttcgatgc catgatccag ccggtgtacc ggcaagtgca ttggtgattt
      181 acctgcctcg aacaggctca taccctcgct gccgcgagta atgagaacgc tcattgcatg
      241 gctgccatcg agcaaccgtt gcccggcttc ttccagctca gcctgtgaag tgattgtcat
      301 acccagtgtg agttcagctt ccggctgatt cggcgtgagc gctgtgacgc cctgaaaacg
      361 gaaaagcgag ccatgcgaat caaccaccac cactttatgc agttctcgcg ccatcggaat
      421 acacgtttca ataatttcgg ggctaatgac cccgttctca taatctgaaa ggatcatcgc
      481 gtcgatagaa ggcaacatgt gctggatgta ttcgcgaata tttcctttgg ccggttgacc
      541 aacttctgac gtatcaacgc ggtcaatgcg cacgatatgc tgctgcacaa tctgaggact
      601 accggcgagg atgcgcgtct tcgtggaggt agggcgctga gcatcggtca caaggccctc
      661 ctggcgcatc cctagctcag cgacagcgcg ccgcaaacgc ttgcccggca catcatcgcc
      721 gacaccacca gcgagaaaaa cctccgcgcc cagggtacga gcattgacgg cgacattagc
      781 cgcgccgcct ggaatagtac gctcatcgtc caattccagg atcaacacgg gcgcttcacg
      841 agcgatacgt gttggtctac cgatcaagta ttcgtctgca accatgtccc cgatcaccag
      901 cacgcgctta cttgcaaaac tggaaacgag cgcgtgcaat cgcgattcgg ctatatgaaa
      961 atcatgggcc atgctttttg cgccctttca atagaacctg tatgataaaa cgaggtatcg
     1021 ccaacatgcg ttgccagcgc cagggctcgc gatagaggcg atatagccat tcaaggccca
     1081 agcgctgcat cgctcgcggc gcgcgttgtt ggcgacccga gagaaaatcg tatgctcctc
     1141 ctacacccat tgctactgcc actggcaaac gcgagagatt gcggtgtatc cagagatcct
     1201 gcgcgggagc gccgtaggcc acacagagta catccgcgtt ggccgtgcga atacgctcaa
     1261 gaatagcatc ttcttcggcg ggagcaggtg aacccgcgta ggtaccggca atttgcagcc
     1321 ctggcgcgag tgtttgtaaa catacacctg ctagttctgc aactcccggc gcggcgccaa
     1381 gcaagtagag acggtagcct ttctccgcac aacgttttgc taatgctact aacgtatcgg
     1441 tgccggtaat gcgttcgggc gcgggagcgt gtacgaagtg tgttgcccag acgaccccga
     1501 tgccatcggc aaccacaagc gcggcgctgt tgattgcttg acgaaagtct gtattctgct
     1561 gagccgccat cacaaattcg gtgttgacgg tgacgatttg ctggcaaggc agcgcattat
     1621 cattggtacg gcgtgaggcg atcaactgct cgatcatatc gagcgcctgc cgctgggaaa
     1681 gacggtctac ccgcacgccc aggacataag ccggcgcggg tgggctttgt tgagacatct
     1741 tttctaactg tatcgacatt cttctttatt taccagttga ttatttccgc ttcggcatcg
     1801 gtcacaatga catccgggcg tgtttcctcg atatagcgcc ggacgcgccc cagtatttcg
     1861 tcaatatgct ggctactatt gctaacgcag ctcacgccaa gtttagcgat ttgccagcgg
     1921 tcttgctcat cgacttccgc aatcgccacc tcaaactgct ggcgaacccg tgccatgact
     1981 gatttgatga cctggcgttt atcttttaac gagtgacatt ccggcaggtg cagtgtgatc
     2041 tggctcactc caactttcat gtcgctagta tagcatagta ctgtatagca gaagcaagac
     2101 cattacgata cactggcgat tggcgctttg ctcgttcata caaatgaaac cttttgccca
     2161 gcgtcagatt cgacgctctc tagccaactc tggtaaaatg gattgagtac aatagtgaaa
     2221 ccaaaggaga gtatttccat gactgaaaca actacttcga gtggagggcg tcccagcctg
     2281 cctatgcaag gcataccgca gccaagacgc gatgaggtgc tggatctacc tcgcgtgcat
     2341 atttcttccc atccggtaat ggctcataaa atgacggcat tgcgcgataa aaacacgcaa
     2401 ccaccggagt tttaccgcct ggtaaaggag attggtgcgc tgctagccta tgaagcaact
     2461 gccaccctgg cgcttgagac tgtcccgata gagacaccca tgggtccaat ggttggcgaa
     2521 cgcctggcag gcggtatcgg cgtgacacca atcttacgtg ctggccttgg cctggcggag
     2581 ggatttcgcg aggttatccc cgatgcgcaa atctggcacc tgggtctgcg ccgtgacgag
     2641 gaaacgctgc aggcgattgc gtactacaat caacttcctc accaggtaga cttgcaagta
     2701 tgctacgctg ttgaccccat gcttgccacc ggcggctcag ccatcgacgc gatcaacgta
     2761 ctcaagagac gagatattcc gcgtctctgc tacgtaggca ttatagctgc cccctacggt
     2821 ttgctcaagc tctctcagac ccatcccgac attgatattt atatcgcggc cctcgatgag
     2881 agcctgaatg atgtcggcta tatcctacca gggttaggtg atgctggaga ccggcaattt
     2941 ggcacatttt aaaccagccc tgcatagtta agaaggtagt tgatggcatt tccattctct
     3001 ccaagaaatg aaggagacga cgaggaagta acagatagta cggatgaggg actagaattt
     3061 ccttcaggag accaggttac agcagaagag caaccctcgg cagaagaaac tcccgcaact
     3121 caacttccgc cggaggcgca gggagaggta aatggcgggc cgctcggctg ctgcctgggg
     3181 gtgactatcg gcctgctgct cagtctaact gtggccatct tgagtcgctt atacgctgat
     3241 ccgcttgccc agatatttca tagcagcctc tctataaccg ttcgtattgt catgtctctt
     3301 cttgcaatcg catgcgcagt gctcttt
//
LOCUS       DASZAK010000163         4451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_394467, whole genome shotgun sequence.
ACCESSION   DASZAK010000163 DASZAK010000000
VERSION     DASZAK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394467"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1509
                     /locus_tag="VGT44_02785"
     CDS             136..1509
                     /locus_tag="VGT44_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579754.1"
                     /translation="MQQHDQTDPVTTSKTASVDTMQDELHANEESADKAVISVLKRSG
                     DNRGGFDWLALSISVLIAAIFSVAGRLYAQPTTSLVPVAVLSASGCGLLITALRKLRT
                     SKGSGVLEAALAGLCVALLQFLAALSYPGVEPSLGNEQLAGPGFFSTWGLIAIFAVLF
                     SMIGATLGHLAFAPLRPLPAKNGRQQSRTQAQHVHTNLVDEDTQASQEQQTQEAPLEV
                     DAAPSLAQDTPVEVHEETDHESEQIDEGEASPSAQSAASQRTLVSYAITVLMLGFIPM
                     LAGYVFAAAFDFTLSRNGYAPGPFPTLRLLSSLLPWQVPDPVALQGTGSVSVTLSLLW
                     RIPLFFGNPTPFDAQALEPYIFNAAGLGTFLVISLKRENTSIPFKLLWGKLALFEALL
                     GLAIVLPAAFWMLQGLQGVFEFHTVVIALGTLRLLDTPNVVLDLLTSLLVCIVTGILV
                     GTRTRRN"
     gene            1770..2087
                     /locus_tag="VGT44_02790"
     CDS             1770..2087
                     /locus_tag="VGT44_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579755.1"
                     /translation="MQHLRDTATTISSSTTFYSSRFGFTSVCISITYTIASARTNAQN
                     TAGHQTRTYSSITGCAIFAAGQADRSIARDNSSHCLVIIGSPCPATFYCKHSTHTANL
                     PGQ"
     gene            complement(2081..2266)
                     /locus_tag="VGT44_02795"
     CDS             complement(2081..2266)
                     /locus_tag="VGT44_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579756.1"
                     /translation="MQVSPVERIVVVEPAGAVRLVRAVEAVALAVLVALMAPELLYGP
                     AVAMGMVARELAAPEVY"
     gene            2314..3804
                     /locus_tag="VGT44_02800"
     CDS             2314..3804
                     /locus_tag="VGT44_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579757.1"
                     /translation="MGRQPDQLKTSHPASETPTRLLPGDLPDTPSPESLPTLHLANGS
                     QSPMQNGSHHEMPIFSPESLNYTSKAAEHWRSSWQDRQYAEAGPAEDVSKGQASVPSP
                     LNAMQHSFVRMRAVILKQKEDKRKVSFGFWITLLIMLCLIGGMGAYIIYSYLPNSSFN
                     AAHIAQPAAGQSPALFIVGAKSSTFKIGQTLYIAGSHFAAHDVITFMLDTTTPIMSNG
                     TPLRAQANAQGAFNVTIKVGKDWPIGDRIIQAVDATANTSSYLNIQIIPAAKPVTNSP
                     NLSIALNGQPLSLLQFTNQVGHADPPSQRITITNTSNAVLQWSASVNTDWLVIEDNNN
                     SGQLAIAEPQTIGIGVVTSGLSATLPNAPYVGQINFIINNNEVLTLRVQLTITVVTPE
                     LSFSPSPVVGIAKNGSCLDGTLTLINLGSAVVTWAVNPDSSATSFIQFVSSLGKRAPS
                     GSLNPSGMNGDTVILTLQCTGVKAGQSFHISIYGNGIPASDTVQIE"
     gene            3858..4361
                     /locus_tag="VGT44_02805"
     CDS             3858..4361
                     /locus_tag="VGT44_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579758.1"
                     /translation="MDNPTLLTILSTAFILLGAYNLFSAARRAREMKLAGQQFKWMKQ
                     INALVGIEYLLLAWVFLLTNLDRNSSIPSSVKTIILPLYLICLLAAAIFAGLVIYRGI
                     FNARTIRAQAARSAANRLTSDTTSRALKSTSLQNEVTGTLADSNDRRRERRKNAAAAR
                     RRRAGKA"
BASE COUNT         1043 a         1348 c         1098 g          962 t
ORIGIN      
        1 ctcacaacgt tcccataccg gtgtagaggg taggggcgat gctggccatc gccctggcgg
       61 taatcacttc ctcttgtcgc attggagaga ttgtgctagt atccatgaag acagaagaag
      121 gagcgaacat agccggtgca acaacatgat cagactgacc ccgtaacgac atctaagacc
      181 gcatccgtcg atacgatgca agacgagtta catgcgaacg aggaatctgc cgataaggcc
      241 gtcatttccg tcttgaaaag gtccggcgac aatcgaggcg ggtttgactg gcttgcgctc
      301 agtattagcg tcctgattgc ggccattttc tccgtcgcgg gtcgtctgta tgcccaacca
      361 acgacctcgc ttgtgccggt agccgttctc agcgccagcg ggtgcggatt actcatcacg
      421 gcgctcagga aattgcgcac cagcaaaggg tcaggagttc ttgaagcagc cctggcgggt
      481 ctgtgtgtcg ccttactcca attcctcgcc gcgctttcct atccgggcgt tgagccttcg
      541 cttggtaatg agcagctcgc gggtccaggc ttcttttcaa cctgggggct gatcgccatc
      601 tttgcagtgt tgttcagtat gatcggcgca acactcggcc acctggcctt tgcgcctttg
      661 cgaccattac cagccaaaaa cggccgccag caatcacgaa cacaggcgca acacgttcat
      721 acaaaccttg ttgacgagga cacacaggca tctcaggaac agcaaacaca ggaagctccg
      781 ctagaagtag atgcagcgcc atcactcgcg caagatacgc cggttgaggt tcacgaagaa
      841 acagatcatg aatccgagca aatcgatgaa ggcgaggctt cgccgtcggc tcagtcagcc
      901 gcatcacaac gcacgctcgt gagttacgcc attaccgttc tgatgctcgg ctttatcccc
      961 atgctggctg gctacgtatt tgccgccgct tttgatttta ccctcagccg caatgggtac
     1021 gcgcccgggc ctttccccac tttgcgcctg ctgagcagct tgctaccctg gcaggtgccg
     1081 gaccccgtcg cgctacaggg aaccggttcg gtctcagtca cgctgtcatt gctctggcgc
     1141 atcccgctgt tcttcggcaa ccctacgcca ttcgacgcac aggccctgga accatacatc
     1201 ttcaacgctg ccggactggg taccttcttg gtgatcagtc tcaaacgaga gaatacgtct
     1261 ataccattca aactcctctg gggcaaactg gcgctgttcg aggcgctgct tggcctggcg
     1321 attgtgttac ccgctgcctt ctggatgttg caggggttgc aaggggtctt cgagtttcac
     1381 accgttgtta ttgcgctcgg cacactgcgc ctgctagata caccgaacgt tgttctcgat
     1441 ctcctcacca gcctgctggt gtgtatagtc accgggatac tggtcggcac gcgcaccagg
     1501 cgcaactgat gcccaccttc aaacaacccc actagcacaa atagcacaga atgatatact
     1561 cccatccatc tatacgttat ggtagacaga ggcaacgatg tgcttgcatg gcacaaacgc
     1621 gccttgcatc gtttcggggt tcgacgcggc tcagcaggat acgaggggga gtgaactgta
     1681 tggctctact gaccgctttc atctatggaa gaattgacca tgaaccgttg tccaaaatgt
     1741 aagaatgaac tggtagcgga agcgcgcttt tgcaacatct gcgggacacc gcaacaacca
     1801 tcagtagcag cacaaccttc tacagcagca gattcggctt cacaagcgtc tgcatctcaa
     1861 tcacctacac cattgccagc gccagaacca acgcgcaaaa tacagccggt catcaaacgc
     1921 gtacctattc cagcatcacc ggctgcgcca tcttcgcagc cggacaagcc gaccgttcca
     1981 tcgccaggga taattcgtcc cattgtctcg tcatcatcgg ttcgccctgc cctgccacct
     2041 tctactgcaa gcattccacc cataccgcaa atctccctgg tcaataaacc tccggtgcag
     2101 cgagttccct cgccaccatc cccattgcca cagccggtcc atagagcaat tcaggggcca
     2161 tcaacgcaac tagcacagcc agcgcgaccg cctcaacagc ccgtacaagc cttacagccc
     2221 cggcaggctc aacaaccaca atccgctcaa caggtgaaac ctgcacaatc cattcgatca
     2281 gcgcctataa cacctgcctt tccagagcaa ctggtgggca ggcaaccgga tcaactcaag
     2341 acaagtcacc cggcttcaga aacgcctact cgtctgttac ctggcgattt gccggacact
     2401 ccatcacccg aatcgctgcc cacgctgcat ctcgccaacg gctctcaaag tccgatgcaa
     2461 aacggctcac atcacgaaat gccgatcttt agccccgaaa gcctgaatta tacgagcaag
     2521 gcagctgaac attggcgaag tagctggcaa gaccgccaat acgccgaagc cggacctgcc
     2581 gaagatgtct caaaaggaca ggcgtcggtt ccttcgcctt tgaatgccat gcaacatagc
     2641 tttgtgcgaa tgcgcgccgt gatcctcaaa cagaaagaag ataaacgcaa agtgagcttc
     2701 ggtttctgga ttacactgtt gataatgctc tgcttgatcg gtggtatggg tgcttatatc
     2761 atctattcgt acctccccaa ttcctcgttc aacgccgcgc atatcgcgca gccagccgcc
     2821 gggcaatcac cggcgctttt catcgtagga gcgaaatcgt cgacctttaa aatcggtcag
     2881 acactgtaca tcgccgggtc acatttcgct gcgcacgatg tgattacttt catgctcgac
     2941 acgacaaccc ccattatgag caacggcact ccactgcgag cacaggccaa tgcccagggc
     3001 gctttcaatg ttacaatcaa agtcggcaaa gactggccaa tcggagatcg catcatccag
     3061 gcagtcgatg cgaccgccaa tacaagcagc tacctgaata tccagatcat cccggctgcc
     3121 aaacccgtca ccaacagccc aaatttgagc atcgcgctca acggacaacc actctccctg
     3181 ctccaattta cgaaccaggt tggacatgcc gacccgccgt cgcagcgcat taccattacg
     3241 aataccagca atgccgtcct gcaatggtcg gcctcggtca acaccgactg gctggtaatc
     3301 gaagacaaca ataactctgg gcagctcgct attgccgaac cgcaaacgat cggcataggc
     3361 gtcgtaacga gtggattgag cgctaccttg ccaaacgcgc catatgtggg acagataaac
     3421 ttcatcatca acaacaacga ggtgttgaca cttcgggtac aattgacgat aacggtggtg
     3481 acgcctgaac tgtctttcag cccttcaccg gtcgtcggca tagctaaaaa tgggtcctgc
     3541 ctggatggga cgttgacact catcaacctg ggtagcgccg ttgtcacctg ggcggtgaac
     3601 cctgatagca gcgcgaccag tttcattcaa tttgtttcga gcctgggcaa acgcgctcca
     3661 tcgggcagct tgaatccttc cggcatgaat ggggacacgg tgatcctcac gctgcaatgc
     3721 accggcgtga aggcaggcca gtcatttcat atttcgatct atggcaatgg tattccagca
     3781 tctgataccg ttcaaattga gtagccagca accggttact gcatcagcgt tctatgcctg
     3841 ggaggatgag cgatttcatg gataacccca ccttattgac gattcttagc actgcgttca
     3901 tcctcctcgg ggcatataat ctctttagcg cggcaaggcg cgcgcgcgag atgaagctgg
     3961 ctggccaaca gttcaagtgg atgaaacaga tcaatgcgct cgtgggtatc gaatacttgc
     4021 tgctggcatg ggtctttctg ttgaccaacc tcgatcgcaa cagttcgatc ccgtcctcag
     4081 tgaaaacgat aattcttcca ctttatctta tttgcctgtt agccgctgcg attttcgcgg
     4141 gactggtcat ctaccggggt attttcaatg cccgcaccat ccgcgcgcaa gccgccaggt
     4201 cggcagcaaa caggttgacg agcgacacaa cgtcgcgcgc attgaagtcc acttctttgc
     4261 aaaacgaggt gacgggaaca ctggccgatt ccaacgatcg ccggcgagaa cgccgcaaaa
     4321 acgcggccgc cgcccgtcgc cgccgcgccg gaaaagcctg attctacatg ccttcgagtt
     4381 ccagttgtat ctcatctata gcatgttgcg cgtcatactg cgccagaata cgcacgatgc
     4441 ggcaggcgct g
//
LOCUS       DASZAK010000164         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_394597, whole genome shotgun sequence.
ACCESSION   DASZAK010000164 DASZAK010000000
VERSION     DASZAK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394597"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1559
                     /locus_tag="VGT44_02810"
     CDS             144..1559
                     /locus_tag="VGT44_02810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2579759.1"
                     /translation="MNLLVGKMLGGYRLLRLIESGGMGDIYFAQHARIGRKAAIKVIS
                     GDLRAVPNTPEGKQAITRFEREARAVAALEHEHILPLYHFGEEDIEEGAGTITYLVMP
                     YLSEGSLWNWLQRRTQQFGFGWPISAEEAGYYLLHAAAGLQSAHDHGIIHRDIKPTNF
                     LIRVDRSPIKDSARAYLARIFPQGRPHLLLADFGLAAFYRNGRSMHRSVGTPLYMAPE
                     QFDDPSQESSVLVGPAADQYALAVMIYQLVTGRAVFEGTPSQLLHHHHHTPPTPPSYY
                     NRALPTELDRVFLKALAKMPEQRFPSILAFAQEYDHVLFTLRRQVNAGKLEPDNIKRE
                     ALKEEQLAGQVLQAEQAERPVHAHQRSQVTLPLPRQMSFAQVRTPHPRPAVLIASSTK
                     VATLEASKAQKLTPLPREDHPYLREDDASAPTVALPRNKRGMIDLSPKVQLLLLVLAL
                     LALLAISVLLIALFVSHAHTA"
     gene            1657..2418
                     /locus_tag="VGT44_02815"
     CDS             1657..2418
                     /locus_tag="VGT44_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2579760.1"
                     /translation="MLKLLLVDDHGPLRRALREGLQATGAVQVLGEAATGQEAVARAL
                     DLDVDVILMDVQLADPQAGRKALSGVGAAVAIRRERPRMPVVFYSIQDDNEYYREFRA
                     SGILTHYAYVRKSNYLLPNMLVPLLRDAVNGRSLVDPEIEDRVQEVRDLDEQSPRALL
                     EPNELRVAELVAQGMTNEQIAARLNLHDKRAVSRTNGRIYAAWGLSDTAVDDKVARAR
                     ATLIFTSNRMIMWDENGIALVQDRRGTWVPFYKDE"
BASE COUNT          558 a          779 c          717 g          520 t
ORIGIN      
        1 gccttcatgc ccactctatc ccccgtttct gctacccgct gtctttttct taccgcttcc
       61 tcaccgattt ttctaccccg tctttatact gtaagtagga ctgatgcaac tcagactatc
      121 gttttaaggg gaacagagaa atcatgaatc tgctggtggg gaaaatgctc ggtggatacc
      181 ggctgctccg gctcatcgag agtggtggga tgggtgacat atatttcgca caacacgcgc
      241 gtatcgggcg caaagctgcg atcaaagtga tttcgggaga tctacgggcc gtaccaaata
      301 cgcccgaagg gaaacaggct attacgcgct tcgagcggga agcgcgcgct gtggcagctc
      361 tggagcatga acatatcctg ccactctacc attttgggga agaggatatc gaggaaggcg
      421 ccggcacgat tacctacctg gtcatgcctt atctctcaga aggctccctg tggaactggt
      481 tgcagcgccg cacacaacag ttcggcttcg gctggcccat ctcggcggaa gaggcaggat
      541 actacctgct ccacgcggcg gcggggttgc aatcggcaca cgatcatggc atcattcacc
      601 gcgacatcaa gccgaccaac tttttgatcc gggttgatcg cagtcccatc aaagacagcg
      661 cacgcgccta tctcgccagg attttcccac agggcagacc ccatctgctg ctagccgatt
      721 ttggcctggc cgccttctat cgcaatggtc gctcgatgca ccgatccgtc ggaacaccac
      781 tctatatggc cccggaacag ttcgatgatc cctcgcagga atcgtcagta ctcgtgggac
      841 cggccgcgga tcaatacgcg ctggcggtga tgatctatca acttgtcacc gggcgagccg
      901 tcttcgaggg aacaccgagc cagctgctgc accatcatca ccatacgccg cctacgcccc
      961 ccagctatta caaccgggcg ttaccaaccg aactcgaccg ggtcttcttg aaagcgctgg
     1021 cgaaaatgcc ggagcagcgc ttcccatcta ttctggcatt cgcgcaggaa tacgatcatg
     1081 tcctgttcac acttcgccgc caggtcaatg caggcaaatt agagccagac aacataaagc
     1141 gcgaagcact taaagaggag caactcgccg gacaggtact gcaagccgaa caagccgagc
     1201 gacctgtgca tgcacaccag cgcagccagg tcacgctgcc gcttcccaga caaatgtcgt
     1261 ttgcccaggt gcgcacacca catcccaggc cggccgtctt gatcgcctcc tcaacaaaag
     1321 ttgccacact ggaagctagc aaagcgcaga agctcacgcc gctgccgcgt gaagaccatc
     1381 cttacttgcg cgaggacgat gccagcgcgc caacggtagc gctgccacgt aataaaagag
     1441 gcatgatcga cttatcgccc aaagtccagt tgcttctgct cgtcctggcg ctcctggcac
     1501 tgctcgctat cagcgtcctg ctcatcgcac ttttcgtcag ccacgcgcat actgcgtagt
     1561 ttgagacaca cgtggttgcg ttccgagaac tatcaccgtg ctactcaaag ctggtataat
     1621 aatgctacca tccaataaca tttgagaatg aatactatgc tgaaattatt actggttgac
     1681 gatcatggac cgcttcgccg cgcgctgcgc gaagggttgc aggcgaccgg cgcggtgcaa
     1741 gtgcttggcg aggcagcgac cgggcaggag gcggtggcgc gcgcactcga cctggatgtt
     1801 gatgtgatct tgatggatgt acagctcgcc gacccgcagg cggggcgcaa ggcattgagc
     1861 ggcgtcggcg cggcggtcgc catcaggaga gaacgcccgc gaatgcccgt tgtattctac
     1921 tcaatccagg acgataacga gtactatcgt gaatttcgcg cctccggcat tctgacgcat
     1981 tatgcgtatg tgcgcaagag caattatctc ctgccgaata tgcttgttcc cctgctgcgc
     2041 gatgccgtca acgggcgcag cctggtcgat cccgaaatag aggaccgcgt gcaagaggtg
     2101 cgcgacctgg atgagcaatc gccgcgcgcc ctgctggagc cgaacgaatt gcgcgtggcg
     2161 gaactggtgg cgcagggcat gaccaacgag cagatcgccg cgcgcctcaa cctgcacgat
     2221 aagcgggcgg tcagtcgcac caacgggcgc atctacgccg cctgggggct gagcgatacg
     2281 gcagttgatg acaaggtggc gcgcgcgcgc gccacgctga tctttacatc gaaccgcatg
     2341 attatgtggg atgaaaacgg catagcgctc gtccaggacc gccggggaac ctgggtgccg
     2401 ttctacaaag acgaataagc catggatttc gctgcctttt tacaaccggc ttacctgctg
     2461 caaaccatcg tactcgcgct ctccatcttc aacctggtgg cattcctctg gttggcctgc
     2521 accgtctggc tgaacggcga ccgcagggcg ggcatcacgc gcctgggcgt catc
//
LOCUS       DASZAK010000165         9334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_110876, whole genome shotgun sequence.
ACCESSION   DASZAK010000165 DASZAK010000000
VERSION     DASZAK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9334
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110876"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1895)
                     /gene="glmS"
                     /locus_tag="VGT44_02820"
     CDS             complement(24..1895)
                     /gene="glmS"
                     /locus_tag="VGT44_02820"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV2579761.1"
                     /translation="MCGIIGYVGPQGTDVTTILLEGLRKLEYRGYDSAGIAVLTSTGH
                     LDVRRRAGKLANLAATVEDNARPHPGSLGIGHTRWATHGRPNDTNAHPHPDCTGVLTL
                     VHNGIIENYAELRGELEEQGHRFHSETDTEVLSHLVERFYFGEAQHDLEQAVRLALGC
                     VTGSYAIAVASSEQPDLLVGARLGGGPLIAGVGDGEQFLASDIPAILKHTRRVLILDE
                     GEMVTLRPAGVAITRLDGTPVAREPLTIEWDAESAEKGGYPHFMLKEIYEQPEALRRA
                     MTGRVREGQLHLAELEEMCQAGLLADIQRVVIVACGTSFNAGMIAKYVIERWARLPVE
                     VVTAAEFRYADPIVGPHTLCIAVTQSGETADTLVGIRQAREQGAPVIAITNVVASAIT
                     RLADAVLYLQAGPEICVVATKTFITSVTLLYLLGLYLAQQRQALRDDEIRAMLEALER
                     IPGQAQSLLEAAAGPDDVIAPLAKQLSRPTSIMFIGRGVGYPTALEGALKLKEVSYIH
                     AEGLPAGELKHGSIALLDPDTPLIAIATASRVYEKVVSNMQEVRARDARVVAVATEGD
                     TAIRKHADDILYVPATLEALSPLLAIIPLQLLAYHTAVARGCNVDQPRNLAKSVTVE"
     gene            2423..3592
                     /locus_tag="VGT44_02825"
     CDS             2423..3592
                     /locus_tag="VGT44_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HEV2579762.1"
                     /translation="MDLIGHLINGRYKLIDERGSGSFGMVYIARDMLTNIIYAAKVMH
                     LDQTQDEELLERFKREAYILQNLSDPHVVRLVDYGRDGNIYFIMMHHVDGQNLKWYVK
                     TYGPMEVQRAINYILQAAAGLDAAYNQGVVHRDIKPENILVTNKGVVKIADFGLARGM
                     DTPTLTATDKMMGTAYYSAPEQMQHGHSADTRSDLYSLAAVFFECLTGQPPFTGGGMV
                     DIIFKHINEPVPSIRRARPDVPPALEAFFERTLAKQPASRYQTPREFMQALESIQRTI
                     QGPVTRSMQARLVVYTTGQTFNLQEGKLVVGREDPKREIHPDIQINQINEQAATLGRK
                     HATLTFQEGKWLVEDLNSRNKTRLNGSVLPPYEAHPLKSGDALQFGRVEARFEMA"
     gene            3905..4939
                     /gene="rsgA"
                     /locus_tag="VGT44_02830"
     CDS             3905..4939
                     /gene="rsgA"
                     /locus_tag="VGT44_02830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV2579763.1"
                     /translation="MIENPETLTGVVLNGAHGIYDVHTDIGILRCTLRGKLKKDFAHA
                     QSNKSFNQARPRADKLIALSKSQRTALKEQQIEALPTRLTVGDFVKLRQLDGQNGLIE
                     EILPRQSALSRKDVGSTEKRAIKQTLLANLDQVALVFACAQPEPHFGMVDRYLAICES
                     AGIQAVICLNKADLPHPTYVDQAATLYAALGYTVIWTSVLTGQGIEALQMTLRDHTTL
                     FTGPSGVGKSSLVNVIEPGMAIKTGLVSESTGKGKHTTTGSQLYPLSGGGWLADSAGI
                     RALAAWDIAPEDLADCFVEFRPYLGECLYTDCQHLEEQHCAIRQAVADGVIDTGRYRS
                     YERILMGEER"
     gene            complement(4940..5737)
                     /locus_tag="VGT44_02835"
     CDS             complement(4940..5737)
                     /locus_tag="VGT44_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2579764.1"
                     /translation="MHTRHEQQDERQDEQMEEQIEHLQRQVNELTAANLLLQEQIARK
                     EQFAAMIAHELRGPLTPIINYAQIVARPNQRRETIERGTNIIIGQAWRLTRLIGDLLD
                     SSRLTTGQFTLSCSACDVVALVKEVVEQLRPIAPYHSFALDLPETPLTGNWDYGRLQQ
                     ALGNLVDNAVKYSDEETTITVRVWQTPGKAHISVHNMGVTIPSGDIDQLFRPFSRLPG
                     SGSHRGTGLGLYITKCIVEAHGGELRLDQSLNKVAGTTFSFDLPMQP"
     gene            complement(5880..6089)
                     /locus_tag="VGT44_02840"
     CDS             complement(5880..6089)
                     /locus_tag="VGT44_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579765.1"
                     /translation="MAESGFAALQRRQIELTIGELLLTDDFYIRQGIVERLRHLIAHA
                     DPSLDVSLLSEGAQEELRELDLLPE"
     gene            complement(6110..6469)
                     /locus_tag="VGT44_02845"
     CDS             complement(6110..6469)
                     /locus_tag="VGT44_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEV2579766.1"
                     /translation="MPTYEYLCRTCSHRFETRQKMTDEPLTVCPECGGTIRRVLFPAG
                     VVFKGSGFYKTDHANGSGAGENGHAPKSDGAEPAKVGETGVASEKKSADSSSSNSSDA
                     DSSSTKSSESKVAAETK"
     gene            complement(6739..8715)
                     /gene="uvrC"
                     /locus_tag="VGT44_02850"
     CDS             complement(6739..8715)
                     /gene="uvrC"
                     /locus_tag="VGT44_02850"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV2579767.1"
                     /translation="MNEKVQSVLNSLPHKPGIYLMKDAQGTILYVGKAISLYNRVRSY
                     FQESTDLSPKNRSMVAKIEDIDFMVVKNEVEALVLESNYIKQYRPKYNVLMRDDKSYP
                     YIKVSLSEDFPRVYRVRSFARDGNRYFGPYTNSGAVDSTLDLLNKLFSFRTCRYDASN
                     WALPRGGEPTAGWKQKLLPRPCTQYYIHRCIAPCVGYATREEYDAVIRQVILFLEGKH
                     DEVVKTLQAQMEEAAENLRFEEAARLRDHIHAVERILEKQRIISTEGQDDQDVVALAS
                     GDDETCALIFFFRAGKLVGREYFILQGTRESSPGEIMTTFMMQFYESSPHIPTEVIVE
                     AEPADHEALHAWLKDKRKGAVTISAPKRGDKLRLVEMVKQNAQEVLEQQRIKWLSDSQ
                     KTQLALEEIQAALNLAAPPYRIECYDISNTQGTNSVGAMVVFEAGHPKNSEYRRFKIK
                     TVEGPNDFASHQEMLRRRFRRQAGSDSNGAGEPDLPGEEQGEEDGAIGLAAQDREQTP
                     PLQHDWTMPDLVIIDGGKGQLSAAMEVLQELHIDVPTVGLAKENEEIFTPGSPEPIIL
                     PRSSQGLYMVQRIRDEAHRFGITYHRKLRSERTFKSVLDEIPGIGPKRKKALMQQYGS
                     VKAISAASIEDLAALEGMTRDAAEKVKEYIGRSQ"
     gene            8976..>9334
                     /locus_tag="VGT44_02855"
     CDS             8976..>9334
                     /locus_tag="VGT44_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579768.1"
                     /translation="MSSERYQSRPVFPLRTAGENPHITSAVGVQETRGHVRARRSSLF
                     ARTVIWVTGLVCLAFLLGSLAQAWTNSGLMQTLAATQLQTQQAQTTHDALQQQAAHFQ
                     DPSVIEQEAREQLGYVRP"
BASE COUNT         1879 a         2778 c         2677 g         2000 t
ORIGIN      
        1 aacacgtgta gccgtcctct cccctactcc accgtcaccg acttcgccag gttgcgcggc
       61 tgatccacgt tgcatccccg cgcgactgcc gtatggtagg ccagcagttg cagtgggatg
      121 atcgccagca gggggctgag cgcctctaac gtcgcgggga cgtagaggat gtcgtcagcg
      181 tgtttgcgaa tggcagtgtc gccctcggtg gcgacggcca cgacgcgggc atcgcgggct
      241 cgcacctcct gcatattgct cactaccttc tcgtagacgc gcgaggcggt cgcgatggcg
      301 atcagcggcg tgtcgggatc gagcagggcg atggagccat gcttgagttc cccggcgggc
      361 aatccttccg cgtgaatata ggagacttcc ttaagcttga gcgcgccctc taaggcagtg
      421 ggatatccca cgcctcgccc gatgaacata atgctggttg gccgcgaaag ctgcttcgcg
      481 agcggggcga tcacatcatc cgggccggcg gcagcctcca ggagcgactg cgcctggccg
      541 gggatgcgct ccagcgcttc gagcatggcg cgtatctcgt cgtcgcgcaa cgcctggcgc
      601 tgttgagcca ggtagagacc cagcaggtag agcagtgtca ccgaggtgat gaaggtcttg
      661 gtggccacca cgcagatctc cggcccggct tgcaggtaca gcacagcgtc cgcgaggcgc
      721 gtgatggcgc tggcgaccac gttggtgata gcgatgaccg gcgcgccctg ctcgcgggcc
      781 tggcgaatgc cgaccagtgt atcagccgtc tcgcccgact gcgtcacggc gatgcagagc
      841 gtgtgcggcc cgacaatcgg gtcggcgtag cgaaactccg cggcggtcac gacctcaacg
      901 gggaggcgcg cccagcgttc gatcacgtac ttcgcgatca tgcccgcgtt aaacgacgtg
      961 ccgcaggcca caatcacgac gcgctggata tcggccagca ggcccgcttg acacatctct
     1021 tccagttcgg ccagatgtag ctgtccttcg cgcacgcggc cagtcatggc gcggcgcagc
     1081 gcctccggct gttcgtaaat ctctttcagc atgaaatggg gatagccgcc cttttcggct
     1141 gactcggcgt cccattcgat ggtcagcggc tcgcgtgcca ccggcgtccc gtcgaggcgc
     1201 gtgatagcaa caccggctgg ccgcagcgtg accatctcgc cctcgtccag gatcagcacg
     1261 cggcgcgtgt gtttgagaat ggccggaata tccgaggcca ggaactgctc gccgtcgcca
     1321 acaccggcga tgagcgggcc accgcccagg cgcgcgccca ccagcaggtc gggctgctcc
     1381 gaactggcga cagctatggc atacgagccg gttacgcagc ccaatgccag ccgcacagcc
     1441 tgttcaagat cgtgctgcgc ctcgccaaag tagaagcgtt cgaccaggtg cgacaacacc
     1501 tctgtatcgg tctcactgtg aaagcgatgc ccctgctctt cgagttcgcc gcgtagttcg
     1561 gcgtagttct caatgatgcc gttatgcacg agcgtcaaca cgccggtgca gtcgggatga
     1621 gggtgtgcgt tcgtgtcgtt ggggcgtcca tgcgtagccc agcgcgtgtg gccgatgccg
     1681 agcgagccgg gatggggtcg agcattgtct tcgacggtgg ccgcgaggtt ggccagtttc
     1741 ccggcgcggc ggcgcacgtc caggtggcca gttgaggtaa ggacagcaat acctgcggag
     1801 tcatacccgc gatattcgag cttgcgaagt ccttcgagca atatggtagt aacatcggta
     1861 ccctgcggcc cgacgtagcc aatgatcccg cacatgaggt cgggtgtcct ttctgttgtc
     1921 gtaggaacgc gattcatcac gtcctggcgg ccttgcgcgc cccaggagac tgatataggc
     1981 gcgacgaatc gcgccacaag tccctactac gccactggta gcgttttgtc ctggctgtta
     2041 ccacccagat ttgtgcgcta tcttgtcagg gtagtgacgt accctggagg atccccgctg
     2101 aaggtccgaa cagcctccac ctcgtcaacc gtggcttcct cctctaagcc cggcccatgc
     2161 gctactagac aggacgacgt tccgtaaaag acagtatacc attacatacg tgaaaatagc
     2221 gtgaagtgtg tcacatcgcc aattagagag tctgctgcta attctctggc ctttcggcgt
     2281 ataaaagatt agaaatttat gataaaatag agactctcac atgtctattg aaaattgggt
     2341 caaactctat agtcaggcgt gtcgttgccc gctcaaatac gttgcaagca catagccttt
     2401 cggataggaa aagagagcgt aaatggacct tataggacat ctcatcaatg ggcgctataa
     2461 attgatcgat gaacgcggga gcggcagctt cggcatggtg tatatcgcgc gcgacatgct
     2521 cacgaacatt atctacgcgg ccaaagtcat gcatcttgac cagacccagg atgaagaact
     2581 gctcgaacgg ttcaagcgag aagcctatat tctgcaaaac ctgagcgatc cccacgtggt
     2641 gcggctggtc gattatggcc gggacggcaa tatctacttc attatgatgc atcacgtcga
     2701 tgggcaaaat ctcaagtggt atgtcaagac ctacgggcct atggaggttc aacgcgccat
     2761 caactatatt ctccaggcgg ccgcagggct ggatgcagcc tacaaccagg gcgtggttca
     2821 ccgtgacatt aagccggaga atattctggt cacgaacaag ggcgtcgtga aaatagccga
     2881 tttcggcctg gcgcgcggca tggatacgcc gacgctcacc gccaccgata agatgatggg
     2941 aacggcgtac tacagcgcgc cagagcagat gcagcacggc cactcagccg acacacgttc
     3001 cgatctctat tcgctggccg ccgtcttttt tgaatgcctg accggtcagc cgccttttac
     3061 gggcggaggc atggtcgata ttatcttcaa acacatcaac gagcctgtgc cttccattcg
     3121 tcgcgcccgt cccgatgtgc cgccagcgct cgaagcgttt ttcgagcgta ccctggcgaa
     3181 acaacccgcg agccgctatc agacgccgcg cgagttcatg caggcgctcg aaagcataca
     3241 gcgaaccata cagggtcctg tgacgcgctc tatgcaggcg cggctggtcg tctacacaac
     3301 gggacagacc ttcaatttac aagaggggaa gctggttgtt ggccgcgaag accccaaacg
     3361 cgaaattcac ccggatatcc agatcaacca gatcaacgaa caggctgcta cgctgggccg
     3421 caaacacgcc accctcacct ttcaggaagg gaagtggctg gtggaggatc tcaattcgcg
     3481 caacaaaact cgcctgaacg ggagcgtact gccgccttac gaggcgcatc ccctcaaaag
     3541 tggtgacgcg ctccagttcg gcagggtaga ggcgcgcttc gagatggcgt agtaatgcgt
     3601 cacggtaaac cggctcctac tgtactcctg gttcccattg taaaatcacg ctccgtaatg
     3661 tataattcca ctaaccagag gtcatgctag cgtgtgactt ctcatcacgt tgatgtcgct
     3721 taaattctgg cttcgccagt ctcagtagga ggtgcaagta tgggaaagct gctctatgaa
     3781 tcgaccaggc ttgtctgcac cttgtctacc tgccgagaga actagcattg cattccctcc
     3841 gcacggtaga catcagcaca ttgatgatac cgtgtttttt tatgctcctg gagggaagaa
     3901 cgcattgatt gagaaccctg agacgctgac cggcgtggta ttgaacggcg cgcatggcat
     3961 ctacgacgtt cataccgaca ttggcatcct gcgctgtacg ctgcgcggca aactcaagaa
     4021 agacttcgcg cacgcgcaat ccaacaaatc attcaaccag gccaggccgc gcgccgataa
     4081 actgattgcg ctgtcgaaat cacagcgcac cgccctgaaa gaacagcaga tcgaggcgct
     4141 accaacgcgc ctcaccgtcg gcgatttcgt caaacttcgg cagcttgatg gacagaatgg
     4201 gctgatcgag gaaatcttac cgcgccagag cgcattatcg cgcaaagatg tcggctcaac
     4261 cgagaaacgc gctatcaagc agacgctgct cgccaacctg gaccaggtcg cgctggtctt
     4321 cgcctgcgct cagcccgaac cacacttcgg catggtggac cgctaccttg ctatctgcga
     4381 atcagcaggc atccaagcag tcatctgtct gaataaagcc gacctgccgc acccaaccta
     4441 tgtcgatcaa gccgcgactc tgtatgccgc gctgggctat actgtgatct ggaccagcgt
     4501 ccttaccggc cagggtatcg aggcgttgca aatgacgttg cgagatcata cgaccctctt
     4561 caccgggcca tccggcgtag gcaagtcctc gctggtgaat gtcatcgagc caggtatggc
     4621 gatcaagacg ggccttgtca gcgaatcgac cggcaagggc aaacacacca ctaccggctc
     4681 gcagctctat ccgctttcgg gcggcggctg gttggccgat tcggcgggca ttcgcgccct
     4741 cgcagcctgg gatatcgccc ccgaagacct ggcggattgc ttcgttgaat ttcgtcccta
     4801 cctcggtgaa tgtctctata ccgattgcca gcatctcgaa gaacaacatt gcgctatccg
     4861 ccaggctgtc gccgacggcg taatcgacac gggacgctat cgcagctacg agcgcatcct
     4921 catgggtgag gaacgctgat tatggttgca tcggcaaatc aaacgagaac gttgttcccg
     4981 ccactttgtt gagggattga tcgagtcgca gttcaccgcc gtgcgcctct acgatacact
     5041 tggtgatgta gaggccaagg ccggtgccgc gatgcgaacc gctgccgggc aggcggctga
     5101 aggggcggaa gagctggtcg atatcgccgg aggggatagt gacgcccatg ttatgcacac
     5161 tgatgtgcgc cttgccgggc gtttgccaga cccgcacggt gatggtcgtc tcttcgtcgg
     5221 agtacttgac ggcgttatcc accaggttgc ccagcgcctg ctgcaaccgg ccataatccc
     5281 agttgccggt cagcggcgtt tcggggagat caagcgcgaa ggagtgatag ggtgcgatgg
     5341 ggcggagttg ttccacgact tccttgacca gcgccaccac gtcgcaggca ctgcacgaga
     5401 gcgtaaactg gccggtagtg agacgcgagg agtcgagcag gtcgcctatc aggcgcgtca
     5461 gacgccacgc ctggccgatg atgatgttgg tgccacgttc gatggtctca cggcgctggt
     5521 ttgggcgcgc cacaatctgc gcgtagttga taatgggggt cagtgggcca cgcagctcgt
     5581 gcgcaatcat cgcggcaaac tgttctttgc gcgcgatttg ctcctgcaaa agcagatttg
     5641 ccgccgtcag ttcattgacc tggcgctgga gatgctcgat ctgttcctcc atctgctcat
     5701 cttgccgttc gtcttgctgc tcgtgacgcg tgtgcatcgc tacgtctccc tatgacggtg
     5761 ggcgcacgtg atcgttactt tccataatgg gattcgtgct atcaagtata cctggaggca
     5821 gcagcagaat gcaacaaatg agcggtaata ggaggaatgg gtatacagtc gccccggcat
     5881 cattctggca acaaatccag ttcgcgtaat tcttcctgcg ccccttcgct gagcagggag
     5941 acatccaggc tgggatcggc gtgggcgatg aggtggcgca gacgctcgac gatgccctgg
     6001 cgaatataga agtcgtcggt caacagtagt tcgccgatgg tcagttcaat ctgccggcgc
     6061 tggagcgcgg caaatcccga ttcggccata tggccctcac tttccctgct cactttgttt
     6121 cagccgctac tttactctcg cttgactttg tgctgctgct atcggcgtca gaactgttcg
     6181 agctggagct atcggcgctc tttttttcgc tcgccacgcc ggtctcgcca accttcgctg
     6241 gctcagcgcc atcgctcttg ggcgcgtgtc cattttcgcc cgctccagaa ccattggcat
     6301 gatcggtttt gtagaatcct gatcccttga agaccacccc cgccggaaac aacacccggc
     6361 gaatcgtccc accacattcc gggcatacag tcaaaggctc atcggtcatt ttctgccggg
     6421 tttcaaatcg atggctacaa gtcctacaca gatattcata tgtcggcatt cgtcattcac
     6481 ctctagtcac agcgtaattg ttgcggtttc gcttgctcta tatatagcgc gttcctccgc
     6541 gcttgtcaat caactcatat agaggcttat tgtgaatttt tcacagtcaa attggtattg
     6601 tcatgctgag cgcagcgaag catctcaccg cctatccacc gagacccttc gcttcgctca
     6661 gggtgacaat accgaacctg gtggtaaaag tccataatct gtaataatca caacaagccc
     6721 agcggcagga aaattcccct actgcgagcg accgatgtac tccttcacct tttcggcggc
     6781 gtccctggtc ataccttcga gggcggccag gtcttcgatg ctggcagcgc tgatcgcctt
     6841 gacggagcca tattgttgca tcagcgcctt cttgcgtttg gggccgatgc cgggtatctc
     6901 gtccaacacc gacttgaagg tgcgttcgga gcgcagcttg cgatgatagg tgatgccgaa
     6961 acgatgtgcc tcgtcgcgga tgcgctgcac catgtagagt ccctgcgacg aacgcggcaa
     7021 gatgatgggt tcaggcgaac ctggtgtaaa aatctcctcg ttctctttcg ccaatccgac
     7081 cgtcggcacg tcgatatgca attcctgcaa cacctccata gccgcgctca actgcccctt
     7141 gccgccatca atgataacca ggtcgggcat ggtccagtcg tgttgcagag gcggggtttg
     7201 ctcgcggtcc tgcgcggcca gaccaattgc tccatcttcc tcgccctgct cttcaccagg
     7261 caggtcaggc tcgcctgctc cgttactatc gctgccagcc tgcctgcgga agcggcggcg
     7321 cagcatctcc tggtggctgg cgaaatcgtt tggcccttcg accgtcttga tcttgaagcg
     7381 gcgatactca ctgtttttcg gatggcccgc ctcgaaaacc accatcgcgc cgaccgagtt
     7441 ggttccctgc gtgttggaga tgtcgtagca ttcgatgcga tacggcggcg cggccaggtt
     7501 cagggctgcc tggatctcct ccagggccaa ctgcgtcttc tggctgtcgc tcagccattt
     7561 gatacgctgc tgctccaaca cctcttgcgc gttctgcttg accatctcca ccaggcgtaa
     7621 tttgtcgccg cgtttgggcg cgctgatcgt cacagcgccc ttgcgcttgt ctttgagcca
     7681 ggcgtgcagc gcctcgtggt ctgccggttc agcctctacg atcacctcgg tagggatatg
     7741 cggcgagctt tcgtagaact gcatcatgaa ggtcgtcatg atctcgccgg gactgctctc
     7801 gcgtgtgcct tgcaagataa agtactcgcg cccgaccagc ttaccggcgc ggaagaaaaa
     7861 gatcagcgcg caggtctcat cgtcgccgct cgccagcgcg acgacatcct ggtcatcctg
     7921 cccctcggtc gagatgatac gctgcttctc caaaatacgc tcgacggcgt ggatatgatc
     7981 gcgaaggcgc gccgcctctt caaaccgcag gttttccgct gcttcttcca tttgcgcttg
     8041 cagggttttg accacctcgt cgtgctttcc ctcgagaaac aggatgacct ggcggattac
     8101 cgcgtcgtac tcctcgcgcg tagcgtagcc gacgcagggg gcgatgcagc gatggatata
     8161 gtactgtgtg cagggacggg gcagcagctt ctgcttccac ccggcggttg gttcgccgcc
     8221 gcgaggtagc gcccagtttg aggcatcgta gcggcaggtg cggaacgaga agagcttatt
     8281 caataaatcc agcgtggaat cgactgcccc cgaattcgta tacggtccga agtagcggtt
     8341 gccatcgcgc gcgaagctgc gcacacgata cacacgcggg aaatcctcgc tcagcgagac
     8401 cttgatatag ggatagcttt tatcgtcgcg catcagcacg ttgtattttg gccggtactg
     8461 cttgatgtag ttgctctcca gcaccagcgc ctccacctcg ttcttcacca ccataaaatc
     8521 gatatcctcg atcttcgcca ccatgctgcg gttcttcggg ctgagatcgg tcgattcctg
     8581 gaaatacgag cgcacgcggt tatacagcga gatggccttc cccacataga ggatagtccc
     8641 ctgcgcatcc ttcatcaagt agatgcccgg cttatgcggc aacgaattca ggactgattg
     8701 gactttttcg ttcatgcttg ttttccttgt attgctatcc caagtgtagc atagcgatat
     8761 acgagaggca agtatgaatc gatggccagc atcgcctcgg ctctgctggc catcgccctg
     8821 gccgggtgtt catttgtgca aaaaaaagaa acgcatccag cgctgctcct tcacttgact
     8881 tctcgctgaa accagggtag tgttattaat gcccgccaga aaagcgcggg aagctagcag
     8941 cataccacat acttcagcag gaaggaggga ggccaatgtc gtcggaacgg tatcaatcgc
     9001 ggccagtatt tccgctgcgg acggcaggcg agaatccgca catcacttcg gctgtcggcg
     9061 tacaggagac gcgggggcat gtgcgcgccc ggcgcagttc gttgttcgcg cgcaccgtca
     9121 tctgggtcac gggcctggtc tgcctcgctt tcctgctcgg ttcgttggcg caggcgtgga
     9181 cgaacagcgg cctgatgcag acgctggcag ccacgcagtt acagacgcag caggcacaga
     9241 ccacccacga tgcgctccag cagcaggccg cccacttcca ggacccgtcg gtgatcgaac
     9301 aggaggcgcg cgagcagctg ggctatgtgc gccc
//
LOCUS       DASZAK010000166         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_323980, whole genome shotgun sequence.
ACCESSION   DASZAK010000166 DASZAK010000000
VERSION     DASZAK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323980"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VGT44_02860"
     CDS             <1..612
                     /locus_tag="VGT44_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579769.1"
                     /translation="PYLGLCLGMQVAVIEIARHVLGLEGANSTEFDPETPYPVIDLML
                     TQRQIADKGGTMRLGNWVCCLTPGTKAQQAYGEAIVFERHRHRYEFNNEYRKRFEAHG
                     VKVSGRSADNKLVEIIELEHHPWFVGSQFHPEFKSRPTRPHPLFRDFIGACLQRQEEL
                     RQDDFDAFQREVVGASAELAEHLTLHAVHPTHHMSEKADGRLE"
     gene            617..>1636
                     /locus_tag="VGT44_02865"
     CDS             617..>1636
                     /locus_tag="VGT44_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579770.1"
                     /translation="MMYLSQLLGTPVEDAEGVRAGKIIDVIASVGAHQVASSTPVSSL
                     PAPTLIIEGSDDQRWSAPVEAVQWHNEALRLRIPLDQLAQQPEELAPQQVSLARTVLD
                     KQVIDIEHKKAVRVNDICLADDWHILGVDNSTLGLVRRLAPAWLLGARSKRTPATLIP
                     WERIALIGEQEPEDESEPTTEPRLTAQAAPQRQQSGQLAELRPADLADIVHQLTPGQG
                     ARLIEGLDDKTAADTLEEIDTDRQSHILESIDVERAAAILNAMEPDEVADLLARLPEE
                     RSEELLKHMKPEDSEEVQELLEYEADTAGGLMTNSCVAVTSTKTVAEALDELRRSIRE
                     HEMRAP"
BASE COUNT          365 a          535 c          478 g          258 t
ORIGIN      
        1 ccctacctgg gcctctgcct ggggatgcag gtcgcggtca tcgaaatcgc gcgccatgtg
       61 ctgggcctgg aaggcgctaa cagcaccgaa tttgacccgg agacgcccta tcccgtcatc
      121 gacctgatgc tcacgcagcg acagatcgcc gacaaaggtg gcacgatgcg cctgggcaac
      181 tgggtctgct gcctcacccc cggcacaaag gcgcagcaag cctacggcga agccatcgtc
      241 tttgagcgcc atcgccatcg ttacgagttc aacaacgaat accgcaagcg tttcgaggcg
      301 cacggcgtga aggtcagcgg gcgttcggct gacaacaagc tggtcgaaat tatcgaattg
      361 gagcatcacc cctggtttgt aggatcgcag tttcacccgg agttcaagag tcgcccgacc
      421 cggcctcatc ccctcttccg cgattttatc ggcgcctgcc tgcaacggca ggaggagctg
      481 cggcaggacg acttcgacgc ctttcaacgc gaagtcgtcg gggcatcagc cgaactggct
      541 gagcatctga ccttgcatgc tgtgcatccc actcatcaca tgtccgagaa agcggatggt
      601 cggttagaat aagacaatga tgtacttgag tcaactgctc ggcaccccgg tcgaagatgc
      661 agagggtgtc cgagcaggaa aaattatcga tgtcattgca tctgttgggg cgcaccaggt
      721 tgcgtcctcc acccccgtat cctctctccc cgcgccaaca ctgatcattg aagggtcaga
      781 cgaccaacgc tggagcgcgc ccgtcgaagc ggtacaatgg cacaacgagg ctctgcgcct
      841 gcgcatccca ctcgatcagc tcgcgcagca gccggaggaa ctggctcccc aacaggtcag
      901 cctcgcgcgc acggtactcg ataaacaggt catcgacatc gagcataaga aagccgtgcg
      961 cgtcaacgat atctgcctgg cagacgactg gcacattctg ggagtagaca acagcaccct
     1021 gggcctggtg cgccgcctgg cccccgcctg gctgctggga gcccgcagca agcgcactcc
     1081 cgccacgctc attccctggg agcgcatcgc cttgatcggt gaacaggaac cagaagatga
     1141 aagtgagcca accaccgaac ctcgcctgac cgcgcaggcc gctccccaac gccagcaaag
     1201 cggacaactt gccgaactgc gaccagccga cctcgccgac atcgtgcatc agctgacacc
     1261 cggccagggc gcgcgcctga ttgagggact ggacgacaaa acggcagccg acacgctgga
     1321 agagatcgac accgaccgcc agagccatat tctggagagc atcgacgtgg agcgcgccgc
     1381 cgccatcctg aacgcaatgg aaccggacga ggtcgccgac ctgctggcac gcctgccgga
     1441 ggaacgctcc gaggaactgc tgaagcatat gaagcccgaa gattccgaag aggtgcagga
     1501 actgctcgaa tacgaagcgg acacggcggg cggcctgatg accaactcct gcgtcgccgt
     1561 cacctcgacg aagaccgtcg ccgaggcgct cgacgagctg cgcaggagca ttcgcgaaca
     1621 cgaaatgcgc gccccc
//
LOCUS       DASZAK010000167         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_359581, whole genome shotgun sequence.
ACCESSION   DASZAK010000167 DASZAK010000000
VERSION     DASZAK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359581"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VGT44_02870"
     CDS             <1..1196
                     /locus_tag="VGT44_02870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEV2579771.1"
                     /translation="GGGSQTAYIPSQFSKAYNYDGLHNAGLQGEGQTVGVFELDGYAQ
                     SDIQAYAQCFGGGSVPLTNVVLDGFNGQPGAGAIEVELDIEMIISQAPHLSKLLVYEA
                     PNTTQGYNDEFARIVKDRTPVVSVSWGDCESNIGQQEATQENQFFQQAAAQGQTILVA
                     AGDSGSSSCFQGLQGGSFNTALNADDPASQPYVTGVGGTNLSLNTDNSYASETVWNGG
                     FLGGAGGGGISQFWKQPSWQTGPGVQNSYSNGMRETPDVSLDADPATGYPVYCTAGSS
                     CSSSGGGILGGTTGGWITVGGTSAAAPMWAAMVSLANEMAAKQGKKVIGFLNPTIYKI
                     ASGSHYSSDFHDITPPSNTSLPSNNDEIGFNGGAYPVTQNYDMATGWGSFNAAKLAAD
                     LVAVG"
     gene            complement(1263..>2086)
                     /locus_tag="VGT44_02875"
     CDS             complement(1263..>2086)
                     /locus_tag="VGT44_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV2579772.1"
                     /translation="WARGERGAHWHDGPHGHHHEHVTFGPGSFQRGPQGWGNEWTPPW
                     MGGERRGPFGPRFFAARRGRGPFGPGGPFGPGGPFGPGGEGRVFGRGDVKYVVLELLQ
                     ERPMHGYEMIKALEEKSGGFYTPSPGSIYPTLQMLEEGGYVTASEAEGKKVYTITDAG
                     RAMLTERNKQGEEHWGGPPWMRGRHREGQEGRGWHGPRPEVQALRSEAMEVARLFAIA
                     GRKSIQEPEQLAQLRALIERTRTDLQAFIYGNASAAGQPAPTQADQPQTPTVEEA"
BASE COUNT          401 a          725 c          586 g          374 t
ORIGIN      
        1 tcggcggcgg cagccagacc gcctacatcc ccagccagtt ctcgaaagcc tataactacg
       61 acggcctgca caacgccgga ttgcagggcg aagggcagac cgtcggtgtc ttcgagctgg
      121 acggctacgc gcagagcgac atccaggcct acgcgcagtg cttcggcggc ggcagcgtgc
      181 cgttgacgaa cgtcgtgctg gatggcttca acgggcagcc gggcgcgggc gctatcgagg
      241 tcgaacttga catcgaaatg attatcagcc aggcgcccca tctctcgaag ctgctggtct
      301 acgaagcccc caacaccaca cagggctaca acgacgagtt cgcgcgcatc gtcaaagacc
      361 gcacacccgt cgtcagcgtc agctggggcg attgcgagtc gaacatcggc cagcaggaag
      421 ctacccagga aaaccagttc ttccagcagg ccgccgcgca gggtcagacc atcctcgtcg
      481 cggcaggcga tagcggcagt tcaagctgct tccaggggtt acagggcggc agttttaata
      541 ccgcgctcaa cgccgacgat ccagcctcgc agccctacgt caccggcgta ggcggcacaa
      601 acctgtcgct caacaccgat aacagctatg cctccgaaac cgtctggaat ggcggcttcc
      661 tcggcggcgc gggcggcggc ggcatcagcc agttctggaa gcagccatcc tggcagaccg
      721 gccccggcgt tcagaactcc tactcgaatg gaatgcgcga aacgcccgat gtctcgctcg
      781 atgccgaccc cgccacaggc tatcccgtct actgcaccgc cggttcttcc tgctcaagca
      841 gcggcggcgg tatcctgggc ggcacaacgg gcggctggat caccgtgggc ggcacatcgg
      901 ctgctgcccc catgtgggcc gcgatggtct ccctcgcgaa cgagatggcg gcgaagcaag
      961 gcaagaaagt gattggcttt ctcaatccca ccatctacaa gatcgccagt ggctcgcact
     1021 atagcagcga tttccacgac atcaccccgc cgagcaatac gtctctgccc tccaataacg
     1081 acgagatcgg cttcaacggc ggagcctatc ccgtcacgca gaactacgac atggctaccg
     1141 gatggggttc cttcaatgcc gcgaaactcg cagccgacct cgtagcagtc gggtaatcct
     1201 tcgcaacaag aaaccacctc tgatgccggc ggcatcagag gtggtttctt gttgtactga
     1261 gcctatgcct cttcgacagt gggcgtctgt ggctgatcgg cctgcgttgg cgcgggctga
     1321 ccggcggcag aagcgttccc gtagatgaac gcttgcagat ccgtgcgtgt gcgttcgatc
     1381 aaggcccgca gttgcgccag ttgctccggc tcctgaatgg atttgcgacc cgcgatagca
     1441 aacagccgcg ctacctccat cgcctcgctt ctcaacgcct gtacctctgg acgaggacca
     1501 tgccaaccgc gtccctcctg accttcacga tggcgtccgc gcatccaggg cgggccaccc
     1561 caatgctctt cgccttgctt gttacgttcc gtgagcatgg cgcgtccggc atccgtaatc
     1621 gtatagacct tcttcccctc ggcttcgctg gcggtgacgt acccgccctc ttccagcatt
     1681 tgcagcgtcg gataaatcga gccgggactc ggtgtgtaga agccgccgga tttctcttcc
     1741 agcgccttaa tcatttcata gccatgcatt ggccgctctt gcagcagttc aagcacgaca
     1801 tacttcacat cgccccgtcc aaagacacgt ccttcgcctc caggcccaaa cggcccacca
     1861 ggcccgaacg gaccgccagg cccaaatggc ccgcgcccgc gtcgcgccgc gaagaacctt
     1921 ggcccaaacg gcccgcgtcg ctctccaccc atccagggcg gagtccactc atttccccat
     1981 ccctgcggcc cgcgctggaa cgaccccggc ccaaacgtca catgctcgtg atggtgtcca
     2041 tgcggcccat cgtgccaatg cgctccacgc tctccgcgcg cccagg
//
LOCUS       DASZAK010000168         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_146166, whole genome shotgun sequence.
ACCESSION   DASZAK010000168 DASZAK010000000
VERSION     DASZAK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146166"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..>2178
                     /locus_tag="VGT44_02880"
     CDS             163..>2178
                     /locus_tag="VGT44_02880"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579773.1"
                     /translation="METKRCQRCHKLLRLDAQVCSLCGGHDFIAAATRSRQTVKLPLR
                     QVEASIPSNPPASPHRAGHYSGLHPEDQPYQSSFLPVLRPPIASVIISSPVLDEPEAV
                     DPFGEADAYMTMPEPYDPPIEKRRIATFTPLPQLRQQRGPQVVPPQQVYAPEAAAPTQ
                     EEVDVEVPTPPTLPMREPSPLAQRPVPRKREKKGGIIPILLALSCFLFLIATTILTFL
                     LLNNKPVPAAKPILNVYPSLPLRATDLVIISGSGFPANDQVWFTHDGKPLIDQNGKKL
                     EVNVFHSGNFSIQIQIPENWPVGHYTIDAVDQHGISAPVTITIEGPSPAPPKLLLSAS
                     QIDEGADKIGAVTHESLTLTNTGGGLVQWKANSDAAWLTASPNNGKFSGRAPVDIGVN
                     RGTLAAQQYTGHLTFTQLNSQSPISLPLTVSMTVIAPPTPTAPTLTAPNLALSSAALT
                     FSSTPFKNPVGQMLTLQNIGQQALNWTAGATTTGGGNWLLVSPSNGTLFGGAQSIVTV
                     SAVTTGLSAGNYSGTVTFSYAGSFASVEVTLTVALPPAPALAVQPGGGLTFNSYQGQD
                     PPSQSFTISNPGTAPLNWGISEDANGSTYAQVSAKSGTLAAGKSIVIFVTPSIAQLSA
                     ATIKALITVYDTDKGTTVKSQQVALTFVIINQAAITLNLNQISFGAAS"
BASE COUNT          478 a          673 c          573 g          454 t
ORIGIN      
        1 ggtaggggcg accctcgcgg tcgccctggt cccaggcatc tatacgaatg tttcattata
       61 tgccataata tgtaaacagt accgttcgta ttcgaaaaat tgccctttgc tgcgttcatc
      121 ctataggggt tatccggttt gtttcctgca caaggagggg gtatggaaac caagcgctgc
      181 cagcgttgcc acaaattgtt gcgcctcgat gcgcaggttt gcagcctgtg cggcgggcat
      241 gacttcatcg cagcggcgac aagaagcagg cagaccgtca agctcccgct ccggcaggtt
      301 gaggcatcca tcccatccaa ccctcccgcg tcgccgcatc gcgccggcca ttattccggg
      361 ctgcatccag aagaccagcc ctaccaatcg agcttcctac ccgtactgcg ccctcccatc
      421 gcttcagtca tcatctcctc tcctgttctg gatgagccgg aggcggtcga tccgttcggt
      481 gaagcggatg catatatgac gatgcctgag ccgtacgatc cgcccataga gaaacggcgc
      541 atcgccacat tcaccccttt gccacaattg aggcagcagc gcggcccgca agttgttcct
      601 ccacagcaag tctatgcgcc agaagccgcc gcgccaaccc aggaggaagt agatgtggag
      661 gttcctacgc cgccaacgct tcccatgcgc gagccgtcgc cgctcgcgca gcggccagtg
      721 ccgcgcaaac gcgagaagaa gggaggcatc atccccattc ttctcgcgct ctcgtgtttt
      781 ttgtttctga tcgcgaccac cattctcacg ttcttattgc tcaataataa acctgtcccg
      841 gcagcgaaac ccatcttgaa cgtctatccg tcattgccat tacgcgccac tgacctggtg
      901 attatttcag gcagcggttt ccctgccaac gatcaggtgt ggtttacgca cgacggcaaa
      961 ccgcttatcg atcaaaacgg caaaaaactc gaggtaaacg tcttccattc aggcaacttt
     1021 tcgattcaaa ttcagattcc tgagaactgg ccggtaggcc actacacaat cgatgccgtt
     1081 gatcagcatg gcatcagcgc accggttaca attacaatcg agggaccatc gccagcgccg
     1141 ccgaaattgc tactgtcggc cagccagatt gacgaggggg cggataagat tggagcggta
     1201 acacatgaat ccttaacgct gacgaacacc ggcggtgggc tggtgcagtg gaaggccaac
     1261 agcgacgcgg catggcttac ggccagccct aataacggca aattttcggg ccgcgcacca
     1321 gtagatatcg gtgttaatcg aggaacactt gcggcgcagc agtataccgg gcatctgaca
     1381 tttacccagc taaacagcca gtcgccaatt tccctcccgt tgacggtgtc gatgaccgtt
     1441 attgctccgc caacgccaac cgcgccgacg cttaccgcgc ccaacctggc gctttcgagt
     1501 gccgcgctca catttagcag cacacccttc aaaaatcccg taggacagat gctgacactc
     1561 cagaacatag gccagcaggc gctaaattgg acggcaggcg ctaccaccac cggcggcggc
     1621 aactggctgc tggtctctcc aagtaacggt acgctgtttg gaggcgctca gtccattgtc
     1681 acggtcagcg cggtgacgac cggtttgagc gccgggaact attctggaac ggtcacattt
     1741 tcctatgcgg ggtcttttgc ctcggtcgag gtgacgctga cagtcgcttt gccgcccgcg
     1801 cccgcgctgg cggtgcagcc cggcggcggg ctgaccttca actcgtacca ggggcaagac
     1861 ccgccctcac agagctttac gatcagcaat ccaggtaccg cgccgttgaa ttgggggatt
     1921 tcagaagatg ccaacgggtc aacctatgca caggtttccg cgaagagtgg cacgcttgcc
     1981 gccgggaaga gtattgtaat attcgtcacc ccctccattg cgcagctcag cgcggccacc
     2041 atcaaagcgc ttatcacggt gtatgatacc gacaaaggaa ccaccgttaa aagccagcag
     2101 gttgcattga cctttgtgat catcaaccag gcagctatta ccctcaacct caaccagatt
     2161 tcgttcggcg cggcctcg
//
LOCUS       DASZAK010000169         1772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_678986, whole genome shotgun sequence.
ACCESSION   DASZAK010000169 DASZAK010000000
VERSION     DASZAK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678986"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VGT44_02885"
     CDS             complement(<1..1104)
                     /locus_tag="VGT44_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2579774.1"
                     /translation="MPGALLKGRYEVQSISSKDRSTATFHALDRHQGIPVTIRALAPQ
                     FTSAQQRMTFLEDAELAMSLSSDISEPGSVRVIDYGEDGPIVFLVKTDMVVEITDKQP
                     SRRRMHSRIEEEKSAQPPIDPLFAQLEDTDGASSLHNWLAEGDLAYETGRYEEALAAY
                     DEALALRQNDPDLWNSRANVLHELRRYDEEMACYDQALALDPGYVFAWSGRGMTLVEQ
                     NRPEEALMAFDRALVLDASQGIIWQAMSDTLYGMGRYGEALIAIDRALALEPESAAMW
                     DIKGNILRRMKETEQALAMHEHATLLAPHNPLAWFDKANDLRDMKRYAEALGAYDHAL
                     SLDPTMAGAWYNRGNVLAALRLYNDALESYDQAL"
     gene            1477..>1772
                     /locus_tag="VGT44_02890"
     CDS             1477..>1772
                     /locus_tag="VGT44_02890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2579775.1"
                     /translation="MEGIQQTLGGEQEQEQPHVIRILLADDHALVREGTRRLLETESD
                     VEVVGEAANGEEAIEFARQLRPDIAVIDIAMPGIGGIAATRAIKVNCPGTAVLIL"
BASE COUNT          390 a          467 c          499 g          416 t
ORIGIN      
        1 taaagcctgg tcataggatt ccagcgcatc gttatacagg cgcagggctg ccagcacgtt
       61 tcctcgatta taccacgcgc ccgccatagt cggatcgagc gagagagcat ggtcgtaagc
      121 gccaagcgcc tccgcgtacc gtttcatatc gcgcagatcg ttggccttgt cgaaccaggc
      181 caggggattg tggggcgcaa gtagcgtggc gtgttcgtgc atggcgagcg cctgctccgt
      241 ttccttcatt cggcgcagaa tgttgccttt gatatcccac atagccgcgc tctccggttc
      301 caacgccagg gcgcgatcaa tcgcaataag cgcctcaccg tagcgtccca tgccgtaaag
      361 cgtgtcgctc attgcctgcc agatgatgcc ctgggaggcg tccaacacca gcgcgcggtc
      421 gaaggccatt agcgcttctt ctggccggtt ctgttcaacg agcgtcatac cgcgcccgct
      481 ccacgcaaaa acataacctg ggtcaagcgc gagcgcctgg tcgtaacagg ccatttcttc
      541 gtcgtagcgg cgcaattcat gcagcacgtt ggcgcgtgag ttccagagat ccgggtcgtt
      601 ctgccgtaga gctagcgctt catcataggc tgctaacgcc tcttcatatc gaccagtctc
      661 gtaggccagg tcgccctcag ctaaccagtt gtgcagggag gatgctccat ctgtgtcctc
      721 aagctgcgcg aatagaggat cgataggcgg ttgtgccgat ttctcttctt cgattcggct
      781 gtgcatacga cgcctggagg gttgtttatc agtgatctcg acgaccatat ctgtcttaac
      841 caggaacaca atcggtccgt cttctccata gtcgatgacg cgcacactcc ctggttcaga
      901 aatatcggaa gagagtgaca tggccagttc ggcgtcttca agaaatgtca tgcgttgctg
      961 tgcgctggtg aattgaggag cgagcgcgcg aatagtcact ggaatgccct ggtgtcgatc
     1021 gagcgcgtga aatgtggcag tgctgcggtc tttgctgctg atcgattgaa cctcgtagcg
     1081 tcctttcagc aacgctccag gcattggcaa gccgcatcga gcgcagaacc gggatttttc
     1141 acgattgtcg tagtgacagt cgttaacgtc aaggcacatc accatgtgac cgtccccttc
     1201 caaacctgca caggcagttc ctttgcaaaa aatgtgtgcc tctactgtat cgaaaaacct
     1261 atcactttgt caatcatgta aaagggtatt ctcaggcaaa agagtaataa gtagaattgc
     1321 tgtagcggtt gacatcaacc tgtagtatag tgacagggaa gttaggagat cggggatgaa
     1381 atattttcat gtctggatag agagaagtag gggcgcaatg cattgcgttc ctagtaatgg
     1441 gttccttcat caaatggaaa ggttccactt gtggatatgg aaggcattca gcaaaccctg
     1501 ggcggtgaac aagaacagga gcagccgcat gtgattcgaa tcttgctcgc cgacgatcat
     1561 gcgcttgtgc gcgaaggcac acgtcgcttg ctggagaccg agagtgatgt cgaagtcgtt
     1621 ggcgaggctg ccaatggcga ggaggctatt gagtttgctc gacaattacg cccggatatc
     1681 gctgttatcg atatcgccat gccaggaatc ggcggcattg cggcgactcg cgccatcaag
     1741 gtcaactgcc ccggaacggc agtcttgatt ct
//
LOCUS       DASZAK010000170         2221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_465885, whole genome shotgun sequence.
ACCESSION   DASZAK010000170 DASZAK010000000
VERSION     DASZAK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465885"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VGT44_02895"
     CDS             complement(<1..195)
                     /locus_tag="VGT44_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579776.1"
                     /translation="MSGISPSSKEQQKGNPPSPRAKRNVGEKPHQYRELNKLQEEQPM
                     DDIDALIEALRTVYASLEKLK"
     gene            complement(205..>2221)
                     /locus_tag="VGT44_02900"
     CDS             complement(205..>2221)
                     /locus_tag="VGT44_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV2579777.1"
                     /translation="VVSAQETKPATLTGLEKYLGSGLIKGIGPVTARRIVAHFGLDTL
                     DIIEQSCSRLVEVPGIAAKRVAMIERAWAAQKAIKDVMLFLRGHNVSTTYAVKIFKQY
                     GDQAIAVVSRNPYQLATDIFGIGFVTADTIARNIGIAPDSDFRYQSGILYVLQQAAED
                     GHCFLPERELVERVVKQLALPESPVDPTRIGALIIQMAEAKQLIAQSGYGDLAESRIC
                     YAPAFYYTEQALAARLAMFARVPVEANLLPHVQHWIELYTEKRAITLSEEQRHAVELA
                     ATSRLLVLTGGPGCGKTFTTRTIVDLWRAMGKSILLAAPTGRAAQRLAEMTKQEAKTI
                     HRLLAFDPKTMGFRYNDEHPIEAQAVIVDETSMLDLFLAHSLVKAIPPDAQVLLVGDV
                     DQLPSVGPGMVLRDLIDSEQIPVVRLTEVFRQAAASHIVTNAHRINAGQFPRLVPTTK
                     FAESDCLWLEAAEPELGAEGIRHLVSEFLPRHGIDAVQLVQVLCPTTRGAVGTRQLNT
                     LLQQVLNPPKLGKVELVRGGSTLRVGDRVIQQVNDYQREVFNGDIGTITSIDLEEQEV
                     VVQFTERAVTYDYADLSELALAWAVTVHKSQGSEYPVVILPIFMQHYLLLSRNLLYTG
                     LTRAKQLAILVGSTKAIGLAIKRVTDRQRYTALADRLKSRAAPPPGG"
BASE COUNT          395 a          670 c          691 g          465 t
ORIGIN      
        1 tttcaatttt tcaaggctgg catagacagt gcgtaaagct tcaataaggg cgtcgatatc
       61 gtccatcggt tgttcctcct gtagcttatt caactctctg tactgatgcg gtttctctcc
      121 tacatttctt ttcgcgcgtg gggaaggcgg gtttcccttc tgctgttctt tcgatgaagg
      181 ggatattcct gacacatctc tgttttatcc tccaggagga ggtgccgctc gactcttcaa
      241 gcgatccgcc agtgcggtat agcgctgccg gtcggtgacc cgtttgatag caagcccaat
      301 ggccttggtt gatcccacca agatcgcgag ttgcttggcg cgcgtcaggc cggtgtagag
      361 caggttccga ctgagcagca gatagtgctg catgaagatg ggcaggatca ccaccggata
      421 ctcactcccc tgcgatttat gtactgtcac tgcccaagcc agcgcgagtt cggagaggtc
      481 ggcataatcg taggtcacgg ctcgctcggt gaactgtacc acaacctcct gctcttccag
      541 atcgatcgag gtgattgtac caatatcgcc gttgaagacc tcccgctggt agtcgttgac
      601 ctgctggatc acccggtcgc ccactcgcag cgtgctgcct ccacgcacca attccacctt
      661 tcccagtttc ggcggattga gcacctgctg cagcagcgtg ttgagttgcc tggttcccac
      721 cgctccgcgc gttgtcgggc agagcacctg caccagctgc acggcatcga tgccgtgcct
      781 gggcaggaat tcgctcacca ggtggcgtat gccttctgct cccagctccg gttcggctgc
      841 ctccaaccac agacaatccg actccgcaaa cttggtggtc ggcaccagac gtgggaactg
      901 acctgcgttg atacggtggg cgttggtcac aatgtggctg gcggctgcct ggcgaaacac
      961 ctcggtcaga cgcacgaccg ggatctgctc cgaatcgatc aggtcgcgca agaccatgcc
     1021 aggtcccacg ctgggcagct gatcgacatc cccgaccagc agcacctggg cgtcgggcgg
     1081 gatcgccttg acgagcgaat gcgccaggaa caggtccagc atcgaggttt cgtcgacgat
     1141 gacggcctgc gcctcgatcg ggtgctcgtc gttgtagcgg aagcccatcg tcttggggtc
     1201 aaaggcgagc aggcggtgga tggtcttggc ctcctgtttg gtcatctccg cgagtcgctg
     1261 cgcggcgcgc cccgtcggcg ctgcgagcag gatggatttg cccatcgcac gccacaagtc
     1321 cacgatcgtc ctggtggtga acgtcttgcc gcagccaggt cctccggtca gcaccagcag
     1381 gcgcgaggtc gccgccagtt cgacggcgtg gcgctgctcc tcagagaggg tgatcgcgcg
     1441 tttctcggta tagagctcga tccagtgctg cacgtgcggc aggagattgg cctcgaccgg
     1501 cactcgggcg aacatggcga ggcgcgcggc gagggcctgt tcggtgtagt agaaagccgg
     1561 agcgtagcag atgcggctct ccgcgaggtc gccatacccg gactgcgcga tgagctgctt
     1621 tgcctcggcc atctggatga tcaacgcgcc gatgcgcgtc gggtccaccg gggattcagg
     1681 gagcgccagc tgcttgacta cgcgctccac caactctcgc tcgggcagga aacagtgccc
     1741 atcttcggcg gcctgctgca acacgtacag gatgccggac tgatagcgaa agtcagaatc
     1801 gggggcaatg ccgatgttgc gcgcgatcgt gtcagcggtc acgaagccaa tcccaaagat
     1861 gtcggtggcg agctgatagg gattgcgcga gacaaccgcg atcgcctggt cgccgtactg
     1921 cttgaagatc ttcacggcat acgtcgtgga cacattgtgt ccgcggagaa agagcatcac
     1981 gtccttgatg gctttctggg cggcccaggc gcgctcgatc atggccaccc gctttgcggc
     2041 gatgccaggg acttcgacga ggcgggagca ggactgctcg atgatgtcga gggtatccaa
     2101 gccgaagtgc gccacgatgc gcctggctgt cactgggccg atgcccttga tgagaccact
     2161 gcccaggtac ttctccaggc cggtgagcgt cgcgggcttc gtctcttgcg ctgagacgac
     2221 c
//
LOCUS       DASZAK010000171         2636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_572412, whole genome shotgun sequence.
ACCESSION   DASZAK010000171 DASZAK010000000
VERSION     DASZAK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572412"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1133
                     /locus_tag="VGT44_02905"
     CDS             123..1133
                     /locus_tag="VGT44_02905"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV2579778.1"
                     /translation="MSASAKKVTRNTVTYLEAISEAIREEMMRDEAVFLLGEDVGTYG
                     GAFKVSTGMLEKFGPERIIDTPMSEAAIIGSAIGAALMGMRPIAEMQFIDFITCGFDQ
                     IINMASKMYWRSGIPVPMVIRGPSGGGTKGGPFHSASPEAWFFHTPGIKVVVPATTYD
                     AKGLLKAAIRDNNPVLYLEHKLLYRMPELREELPEEDYIVPLGKAITRREGEDMTILT
                     YGAMVHQCLKAAQTLEEEDDLQVEVIDLRSLTPLDRETITASVKRTNKVLIVHEDTLT
                     SGIGAELAAILAEDLFEYLDGPITRVAAPDAPFPYAPPLEAAYLPNVEKILAATRKLA
                     AY"
     gene            1172..2539
                     /locus_tag="VGT44_02910"
     CDS             1172..2539
                     /locus_tag="VGT44_02910"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEV2579779.1"
                     /translation="MPTPVKMPRLGESVAEGTLGAWLKNEGDWVEKDESLAEIITDKI
                     NAELPSPVAGRLAKILVKTDETVPVGADIALIEESADVSASPPADAAPGQDAAPVKSP
                     AQAATLVIEQKRASAGNGARAATSAAARTPHVDEEERQSISPLARRLAQEHGIDLNMI
                     EGTGAGGRVRKEDILAYVAQRQAAPAAAVPAPPMPTMPRPAPEPVYAAPVAAFPPSTT
                     EAGEDVEIVTPSRMRLAIAEHMVRSKRTSPHATTVVEVDMTNIAKWLEKNKEEFRRRE
                     GYSISYVPFVMKAVCEGIRKFPIINSSWTEDNKIIIKKRINLGIAVATDMGLVVPTIY
                     DADNYTLAGLAKLVNAVAQRARANKLTIQDMQNSTFVVNNPGTFGTIISVPIINQPNA
                     AILSMDAVVKRPVVIEDDAIAVRSMMYLCLSFDHRILDGAGAGGFLQAVRTKLQSYGR
                     EIDVY"
BASE COUNT          663 a          712 c          733 g          528 t
ORIGIN      
        1 caggacccgc gctacgtcaa cgcccaggat tcgcgtatag gtcaaccgtt taacactatt
       61 acccaggtta gtttgcctgt aggggcccaa tttattgcac acgaaggggt tgtgggataa
      121 cgatgagtgc gagcgcaaaa aaagtaacgc gaaatacagt tacatacctt gaagccatca
      181 gcgaagccat tcgtgaagag atgatgcgtg atgaagcagt atttctcctg ggtgaggatg
      241 tgggcaccta tggcggagcg tttaaggtga gcacgggcat gcttgagaag ttcgggccgg
      301 aacgcatcat cgacacaccg atgtctgaag cggctatcat tggttcagct attggagcag
      361 cattaatggg catgcggcct atcgcggaga tgcagtttat tgatttcatc acctgcggct
      421 tcgatcagat tatcaacatg gcgagcaaaa tgtactggcg ttctggtatt cccgtaccga
      481 tggtcattcg tggcccatcc ggcggaggaa ctaagggtgg cccattccat tctgccagcc
      541 cggaggcctg gttcttccat acgcccggca tcaaggtggt cgttccggcc acaacctatg
      601 atgccaaggg cctgctcaag gcggctattc gcgacaacaa tcccgtgcta tacctggagc
      661 ataaactgct ctatcgcatg ccggaactac gtgaagagtt gcccgaggaa gattacattg
      721 tgccgttggg caaggcgatt actcgccgag aaggtgagga tatgaccatc ctgacgtacg
      781 gggcgatggt ccaccagtgt ctgaaggccg cccaaacgct cgaggaagag gatgacttgc
      841 aggtcgaagt aattgatctg cgcagcctga caccgctcga tcgcgagacc attactgctt
      901 cggtcaagcg cacgaataaa gtgctgatcg tccacgaaga cacattgacc agcggcattg
      961 gcgcagagct ggccgcgatt ttagccgagg acctcttcga atatctcgat ggcccaatta
     1021 cgcgagtagc cgcacccgat gcgccatttc cgtatgcacc acctttggaa gctgcttatt
     1081 tgccgaatgt ggagaagatt cttgctgcta cgcgcaagct cgctgcttat taacaaaacc
     1141 attaatagac catgaacaaa ggagttcgca tatgccaacg cccgtgaaaa tgccacgact
     1201 aggcgaatcg gttgcggaag gaaccttagg cgcctggctc aaaaatgaag gtgattgggt
     1261 cgagaaagac gaatcgctgg ccgagatcat taccgataaa attaacgccg aattgccttc
     1321 gcccgtcgcc ggtcgcctgg caaaaatcct cgtaaaaacg gacgaaacag tgcccgtagg
     1381 cgcagacatc gccctcatcg aagagagcgc cgatgtctcg gcctcgccac ctgctgatgc
     1441 cgcacctgga caagacgctg ctccggtaaa gagccctgcc caagcggcca ctctggttat
     1501 tgaacagaaa cgtgcaagcg cgggcaatgg ggcaagggca gccacaagcg cagctgcacg
     1561 tactcctcat gttgacgaag aggagcggca gagcatctcg cccctggcac gacgtttggc
     1621 acaggagcat ggcattgatc taaatatgat tgagggaaca ggcgctgggg gacgcgtgcg
     1681 taaagaggat atcctcgcct atgttgcaca acgacaggca gcccccgctg cggcagtacc
     1741 ggcaccccca atgcccacga tgcctcgacc ggctccagaa cctgtctacg cagctcccgt
     1801 ggctgcattc ccgccttcga cgacggaagc aggcgaagat gtggagatag tcacgcccag
     1861 ccgcatgcgg ctggcaatcg ccgagcatat ggtacgcagc aaacgcacat ccccacacgc
     1921 aacgacggtg gtggaggtcg atatgaccaa catcgcaaag tggctggaga agaacaaaga
     1981 ggagttccgc cgccgtgagg gctacagcat cagctatgtg ccgtttgtga tgaaagccgt
     2041 ctgcgagggg attcgcaagt tcccaataat caattcaagc tggacggaag ataacaagat
     2101 catcatcaag aaacgcatca acctcggaat cgccgtcgct accgacatgg gcttagtggt
     2161 gccgacgatc tacgatgccg acaactatac gctggctggc ctggccaagc tagtcaacgc
     2221 cgttgcgcag cgggcacgcg caaacaaact gaccatccag gacatgcaga acagcacctt
     2281 cgtcgtgaat aatccgggca cttttggtac aattatctca gtgccgatta ttaatcagcc
     2341 caatgcggct attctcagca tggatgccgt cgtcaagcgg ccggtagtga ttgaagatga
     2401 tgccattgca gtgcgctcta tgatgtacct gtgtctctct ttcgatcacc gcatccttga
     2461 tggtgccggt gccggtggct tcctgcaagc cgtccgtacc aagctacaaa gctacgggcg
     2521 agagattgat gtgtattagc aaggaaaata actatggcga ccatcattcc cgaacgtcgt
     2581 cctgaatggc tcagggtgcg acccccgaaa ggggaaaact acgaaaacct gaagca
//
LOCUS       DASZAK010000172         1736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_430801, whole genome shotgun sequence.
ACCESSION   DASZAK010000172 DASZAK010000000
VERSION     DASZAK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430801"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..>1736
                     /locus_tag="VGT44_02915"
     CDS             277..>1736
                     /locus_tag="VGT44_02915"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2579780.1"
                     /translation="MTTNTSSTSTPSTPFEPQIIPGISSVGSVSQELVNVLQKIVENA
                     GALLDVTNCSVALLDTKTSMLVTLATLHKTGNAQRNTRFQMNEGVAGWVAEHREPLII
                     DDVSLDPRFKKLGRAPVGSMACVPLIDQGVFIGTLTVSSPERDAFDARKQRMLTIFAE
                     QAVLAITNARQAEVAIRQANQLEMLLRLSQGITTSREVDELYRAILIHVQRLVPSDRA
                     AIYVYNELAQELRPIAEALAASDDEHAGERHRREYPRIKIAGAQGEAIRLQHSTSLIA
                     WAATHQHPMIHTPVKFQQDESCAPGEEPQLAEMAVPLVSKEALYGVLTLQRAEAYISE
                     ELRLIRNLSDMAAAALENIALFQRVRADQEQWRAIWNTSSDGIALLGGDACFIEANPA
                     FGQMVGQPPQQITGVDFLELFSCPDESISAECQGLSHIQTALQDRRALPYIELDLPIK
                     GAVRSIGLSITPVSLASVPFSLMIARDITSIRDATR"
BASE COUNT          391 a          574 c          451 g          320 t
ORIGIN      
        1 cgcgccgaaa atgcccccca gggcggcgcg cgcatcatct tctccctgcc catcccctcc
       61 ttcagccagg aacagatggt cggtatgaat accctgccca tgaagccggt ctagcccaaa
      121 ttgctcatct aacccttctc aagcaggtgg caatggtata cttagatcaa ccatgaccct
      181 attgaaaaca acatctatgc cgataccact ttataactat ctttgcatgt ccataatgct
      241 ctgctagcag gtagcgtaat gatgaaggga tatctaatga ccacaaatac atcgtctaca
      301 tcaaccccgt caactccttt tgaaccgcaa attataccag gcatctcatc cgttgggagc
      361 gtatcacagg agctggtcaa tgtcctccag aagatcgtcg agaacgcagg cgcgctgctc
      421 gatgttacaa actgctcggt ggcgctgtta gatacaaaaa ccagtatgct cgtaaccctg
      481 gcgacactgc acaaaaccgg caacgcgcag cgcaacacgc gctttcagat gaacgagggc
      541 gtggccggct gggtcgccga acaccgcgaa ccgctcatca tcgatgatgt cagcctcgat
      601 ccgcgcttca aaaaactggg gcgagcgccg gtcggctcga tggcctgcgt cccactgatc
      661 gaccagggcg ttttcatcgg cactctaacc gtcagcagcc ccgaacgcga cgccttcgat
      721 gcgcgcaaac agcgcatgtt gaccatcttc gccgaacagg cggtgctggc catcaccaat
      781 gcgcgccagg ccgaagtcgc catacgccag gcaaaccagc ttgagatgct gctgcgactg
      841 tcccaaggca tcactaccag ccgggaggtc gatgaacttt accgcgccat cctgatccat
      901 gtccagcgcc tcgtcccctc tgatcgcgcc gctatctacg tctacaacga gttggcgcag
      961 gaactgcggc ccatcgccga agcgcttgcc gcgagcgatg atgaacacgc tggcgagaga
     1021 caccggcgcg agtacccccg cattaaaata gccggtgcgc agggcgaggc cattcgcctg
     1081 caacattcca cgtcgcttat cgcctgggcc gccacccacc agcacccgat gatccatacg
     1141 cccgtcaagt tccagcagga cgaaagctgc gcgccaggcg aggagccgca gctagcagag
     1201 atggccgtcc cgctcgtctc aaaagaggcg ctctatggcg tcctgacgct gcaacgcgct
     1261 gaagcgtata ttagcgaaga actgcgcctg atccgcaatc tcagcgacat ggccgccgcc
     1321 gcgctggaaa acattgccct cttccagcgg gtgcgcgccg accaggagca atggcgcgcc
     1381 atctggaata ccagttcgga cggcattgcg ctgctcggcg gcgatgcctg cttcatcgaa
     1441 gccaatcccg ccttcgggca gatggtcggg cagccacccc agcaaatcac cggcgtggac
     1501 ttcctggaac tctttagctg ccccgacgaa agcatcagcg cagaatgcca ggggctaagc
     1561 cacattcaga ccgctctgca agatcggcgc gcgttgccct acatcgaact tgacctgccc
     1621 atcaagggcg cggtgcgctc catcgggttg agcattaccc cagtctcact ggccagcgtg
     1681 cccttctcgc tcatgattgc gcgagacatc acctcgatcc gcgatgcaac acgcat
//
LOCUS       DASZAK010000173         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_501639, whole genome shotgun sequence.
ACCESSION   DASZAK010000173 DASZAK010000000
VERSION     DASZAK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501639"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..888)
                     /locus_tag="VGT44_02920"
     CDS             complement(<1..888)
                     /locus_tag="VGT44_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2579781.1"
                     /translation="MATHDRRYVIIGNGIAGTTAADTLRKNDPNCSIHLLTNEPYPLY
                     NRVSLPRFLQGVIVEQKVMIRDFAWHEQRNIQLVTETMVTHIDIEAKTVETDSGLTLP
                     YDALLVATGGWANPLNVPGAQGTKHIYNFVTLDDTKTIIARMLESRTALAFGGSFISY
                     ELCDGFAMRKLDTTWLMRGPYWLRASLDPDGGEVVDNIARKFGVHVIHGDEIESIVPT
                     NGVPGYVKTKQGREIQADVIGVGLGITLNTGLLANTPAELRGGVVVDEYLETAVPGIF
                     AAGDVAEFYDTTIYRHHTMG"
     gene            complement(980..2491)
                     /locus_tag="VGT44_02925"
     CDS             complement(980..2491)
                     /locus_tag="VGT44_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579782.1"
                     /translation="MSKTQSTTPNEAMPATAEETKHRDGYQWTALSVTTVGALLASIQ
                     GSALLIALPNILAELHATFFTIMWVLLGYLLILTVLTPIVGRLADMWGRKRLYNSGFV
                     AFTIGSLISGLAQPQFHGADLVFGRVVQGVGAALMVTNSTVIVTDAFRKGQVGLGLGI
                     NQIAGAAGFLIGPIVGGLLTELSWRWVFLFNVPLGVFGALWGMWRLREPVRPLERQPI
                     DWFGAVSLSIGLGGLLLGLSMLAFPILSQQVTYALLIVGVLSLVAFIVFEPRIRIPLI
                     QLRLFRSRLFALANLSGLFNGIARGAVLFLLIFFLQGPYGQNPLAAGLMLVPFGAAFM
                     AIGPLSGRLSDYVGSRVLTPIGLSISAIALFALTTIDQHTPYWLLAIYMLLMGGGSGL
                     FMSPNTNAIMSSVEPHTRGAAAGILNMLNNTGQMLSIAIVFPLALSKLPFGAVQQVFI
                     TGGGMGSFASTIPDFLSGLHLAFLISFILSVIAAIVAASRPAHGSRQAEAESA"
BASE COUNT          587 a          765 c          791 g          484 t
ORIGIN      
        1 tcccatcgta tggtgccggt agatggtggt atcgtagaat tcggccacgt cgccggccgc
       61 gaagatgccg ggaacggcgg tttccaggta ttcatcgacc accacgcctc cgcgcagctc
      121 ggcaggtgta ttggcaagca gcccggtatt cagcgtgatg cccaggccga ccccgatcac
      181 atcggcttga atttcgcgcc cctgctttgt tttaacgtag ccgggaacgc cattcgtcgg
      241 aactatcgac tcgatctcat cgccatgaat gacatgaacg ccgaacttcc tggcgatgtt
      301 atccaccact tcgccgccat ccgggtccag gctggcgcgc agccagtagg gtccgcgcat
      361 caaccaggtg gtgtcgagct tgcgcatggc aaacccatcg caaagttcat acgagatgaa
      421 gctgccgccg aaagccagcg cggtgcgcga ctccagcata cgcgcgatga tggtcttcgt
      481 atcgtcaagc gtcacgaaat tgtaaatatg tttcgtcccc tgcgcgcctg gcacattcaa
      541 tgggttggcc cagccgccgg tcgccacgag cagcgcatca tagggcagcg tcagtccgct
      601 atccgtctcg accgtctttg cttcgatatc gatgtgcgtc accatcgtct cggtgacaag
      661 ctgaatattg cgctgctcgt gccaggcgaa gtcgcgaatc atgaccttct gctccacgat
      721 cactccctgc aaaaagcgcg ggagtgagac gcggttatac agcggatatg gttcgttggt
      781 gagcaggtga atgctgcaat tcgggtcgtt cttgcgcagt gtgtccgcgg cagtcgtgcc
      841 agctatgccg ttgccaataa ttacgtaccg tctatcatgc gttgccaaat gcctattctc
      901 cttgtcaaac atgctaatct gtgttgcaag attcgtggcg attttagcac agtttggggg
      961 agaatgctat agaatggcat tacgctgact cagcctctgc ctgcctgctg ccatgcgccg
     1021 ggcgcgaggc ggccacgatg gcggcgatca cgctcaggat gaacgagatc aagaaagcca
     1081 ggtgcaggcc ggagaggaag tcagggatgg tcgaggcgaa actccccatg ccgccgccgg
     1141 tgatgaagac ctgctgcacc gcgccaaagg gcagcttgct tagagccagg ggaaagacga
     1201 tagcgatgct gagcatctgg ccggtgttgt tgagcatgtt gaggatgccc gccgccgcgc
     1261 cgcgcgtgtg cggctcaact gaagacatga tggcgttagt attgggcgac atgaagaggc
     1321 cggagccgcc gcccatcaac agcatataga tggccagcag ccagtagggc gtatgctgat
     1381 cgatggtggt cagcgcgaag agggcaatag ccgagatcga cagcccgatg ggggtcagga
     1441 cgcgcgagcc aacatagtcc gagagtcgcc cgctcaacgg tccgatagcc atgaaagcag
     1501 cgccaaaggg caccagcatc aaacctgccg ccagggggtt ctgcccgtag ggaccttgca
     1561 ggaagaaaat cagtaggaac agcaccgcgc cgcgcgcaat gccgttgaac aggccgctca
     1621 ggtttgccag ggcgaacagg cggctgcgga agaggcgcag ttggatgagc gggatgcgga
     1681 ttcgcggctc aaagacaatg aaggcgacca ggctgagaac gcccacaatc agcagcgcat
     1741 aggtcacctg ttgcgaaagg atggggaagg ccagcatgct gaggcccagc agcaaaccgc
     1801 ccaggccgat gctcaggctg accgcgccga accagtcgat gggctggcgt tcgagggggc
     1861 gcaccggctc gcgcagccgc cacatccccc acagcgcccc gaagacgccg agcgggacat
     1921 tgaagaggaa gacccagcgc cacgaaagct cggtaagcag ccctcccaca atcgggccaa
     1981 tcaggaagcc cgccgcgccc gcgatctgat taatgcccag gcccagaccg acctggcctt
     2041 tacgaaaggc atcggtcacg atgaccgtac tgtttgtcac catcaaggcc gcgccaaccc
     2101 cctgcacgac acgcccgaag acgaggtcgg ctccgtgaaa ctgcggctga gcaaggccag
     2161 agataagcga gccaatggtg aaagccacaa agccgctgtt ataaagccgt ttgcgccccc
     2221 acatatcagc caggcgacca acgatgggcg tcaagacggt gagaatcagc aaatacccga
     2281 ggaggaccca cataatcgtg aagaatgtcg cgtgcagttc agctagaata ttcggcagcg
     2341 cgatcagcag cgccgatcct tgaatcgagg ccagcagcgc tcctaccgtt gtgacggaaa
     2401 gcgccgtcca ttggtagcca tcacgatgct ttgtctcctc cgccgtagct ggcatcgcct
     2461 catttggagt ggtcgattga gttttactca cgatacatcc tttcgcatag tagtgtgttg
     2521 ataggcaaat tactgcgtag gggcgcaatg aattgcgccc ctacacggcg ctattttttc
     2581 tccccggtca aatgattaag ggcaggcagg gcagcctgga tcgcctg
//
LOCUS       DASZAK010000174         3515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_572423, whole genome shotgun sequence.
ACCESSION   DASZAK010000174 DASZAK010000000
VERSION     DASZAK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572423"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGT44_02930"
     CDS             complement(<1..362)
                     /locus_tag="VGT44_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579783.1"
                     /translation="MSQLHLSLLGEPIVKHGEDTLTFSTRKALALLVYLAVEGGTHAR
                     KTLSESFWPELDAEHGRTALRATLFELRKLLEHCHGPGERAHLLVERDTLGFDQDSPL
                     ILDLRLVESASKQVGRGI"
     gene            complement(757..1221)
                     /locus_tag="VGT44_02935"
     CDS             complement(757..1221)
                     /locus_tag="VGT44_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEV2579784.1"
                     /translation="METENKDQEPETASATLVEGMHFVGNIEGFRWDLDAEEETGGVG
                     AGPQPHRLLLLAMAGCTAMDVISILRKKRQQVSGLNVVVQGSRAEQHPKVYTHINVLY
                     RVRGNNVDPQAVERAIELSKTRYCPVIGMLGKVAEVTTRYEVEEETELRPVR"
     gene            complement(1293..2624)
                     /locus_tag="VGT44_02940"
     CDS             complement(1293..2624)
                     /locus_tag="VGT44_02940"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2579785.1"
                     /translation="MQEIPETYIEDEQSVTLPGVASRVPAVIPHVVIVGAGFGGLQAA
                     RALRDAPVRVTVIDRNNHHLFQPLLYQVATAGISPADICAPIRSILRKQKNADVVLGE
                     VTGVDVEAKLVLMQDQSIPYDYLILATGARDSYFGHNDWAQFAPGLKSIVQATAIRRA
                     ILLAYEKAERETDPEKRQALLTFVLVGAGPTGVEMAGSIGGLAYKTLVSDFRSIDPGQ
                     TRVVLVEALPRVLATFPEPLARKAHTALTRLGIEVRTNAPVEAIDSEGVVIAGERLAA
                     RTVIWTAGVAASPAGEWLGAETDRAGRVKVGSDVTVPGHPDIFVIGDTANFMEDGKPL
                     PGVAQVAIQQGLYAASVIADRVAGKAHTEAFHYVDKGSMATVGRFYGIVSVGKFQTAG
                     VLGWFLWLALHLMFLIGFRNRLVVLFQWLVYFTTFQRGARLITFEDTIQYQ"
     gene            complement(2744..>3515)
                     /locus_tag="VGT44_02945"
     CDS             complement(2744..>3515)
                     /locus_tag="VGT44_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEV2579786.1"
                     /translation="MQREIGRHVADLIEDGATLQLGIGAIPDAVLQELTDRRDLGIHS
                     EMFSDGVLDLVERGVITGSRKALDRGKIVASFIIGSRRLMDFLDDNPMVELRSISYTN
                     DIEVIRQFDHMVAINSALEVDLTGQVCAESIGTAQYSGVGGQIDFLQGAADAPHGRPV
                     LAFPATARAPQAREGNMPYELPSIDGQVSRIVPTLTPGAVVTTTRAHVHYVATEYGRV
                     MLFGRSTADRARLLISIAAPQFREQLERAAYDLHLLNR"
BASE COUNT          723 a         1069 c          965 g          758 t
ORIGIN      
        1 tcaatcccgc gccctacttg cttactggca gattcgacga ggcggagatc caggatgagc
       61 gggctgtcct gatcgaatcc aagcgtatcg cgctcaacca gcaggtgagc ccgctcgcct
      121 ggtccgtgac agtgctcaag gagcttgcgt agctcgaata gggtagcacg taaggctgtc
      181 cgtccgtgct cggcgtccag ctctggccag aacgattcgg acagcgtttt gcgcgcgtgc
      241 gttccacctt ccacagccag gtagacgagc agggccagcg ccttgcgtgt ggagaaggtg
      301 agcgtgtcct caccatgctt cactattggt tcgccaagca gcgacaagtg taactggctc
      361 atcatctact cctgttgtcc actggacgat aacgacagaa aaggttctgc cattccatct
      421 tccctcgcgg atgcgtcgtg atcacagctt agctttgcag gcgaccctgc tggtagagcc
      481 agcggacaaa cgacagccgt ttgtatttgg gcaaagaagg atataaggtg ttgggcgacc
      541 tcctcaggat gttctggagg cgagccagtg tccagcgttg gcaatgacga cactttcccc
      601 tgagtcgcat actgctgaac ttgctttcca acctctgccc cctttctatg gtctcctccc
      661 atcgcaagaa cgggtatgga gagttttttc tgacggagtt gttggacatg tggaacatcc
      721 tgtgacagag tgctgtagta actgattgtc ttccccttaa cgaaccggcc gcagttcggt
      781 ctcttcctca acttcgtagc gcgtggtcac ttccgcgact ttgccaagca tcccgataac
      841 cgggcaataa cgggttttgg agagttcgat cgcccgctct acagcttgag gatcgacatt
      901 gttgccccgc acccggtaga gcacattgat gtgcgtgtag accttcggat gctgctctgc
      961 ccggctcccc tgcacgacta cgttgaggcc gctgacctgt tggcgtttct tgcgcaggat
     1021 cgagatgacg tccatggctg tgcagccggc catcgccagc aggagcaagc gatggggctg
     1081 gggaccggct cccacgccgc cggtctcctc ctcggcgtca aggtcccagc gaaagccctc
     1141 tatatttccg acaaagtgca tgccctctac cagggtggca gaggcagttt ccggctcctg
     1201 gtctttgttc tctgtttcca cgtgcgatct ccttgcgttt tcataaaatc atatatgcat
     1261 gaatcattat agaaataaga acgaacgcaa aactattgat actgtatcgt gtcctcgaag
     1321 gtgatgaggc gcgcaccacg ttgaaacgtc gtgaagtaca ccagccattg aaacaggacc
     1381 accaggcggt tccgaaaacc gatcaggaac atgagatgga gggccagcca taagaaccat
     1441 cccaacacac cagcggtctg aaacttccca accgagacga tgccatagaa gcgtcccacc
     1501 gtagccatac tccccttatc gacgtagtga aatgcctctg tgtgcgcttt gccggcgacc
     1561 cgatcggcaa tcacggaagc agcatagagt ccctgctgga tcgcgacctg ggctactcct
     1621 ggcaggggct tgccatcctc cataaaattc gccgtatcac caatgacaaa gatgtcggga
     1681 tgaccaggga cagtcacatc gctgccgacc ttgacgcggc ctgcccggtc tgtctcggcc
     1741 cccagccact ctcctgcagg agaggccgcc acccctgctg tccagataac cgtcctggcg
     1801 gccagacgtt ccccggcgat cacgacccct tcactgtcga tcgcttcgac tggagcattc
     1861 gttctcactt cgatgcccag gcgggtgagg gccgtgtgcg ctttgcgcgc gagcggctca
     1921 ggaaaagtgg ccaggacgcg cgggagcgcc tcgaccagca caacacgagt ttgtcctggg
     1981 tcgatagagc ggaagtcaga caccagggtt ttatatgcca ggccaccgat cgaccccgcc
     2041 atctcgaccc ccgtggggcc agcgcccacc aggacaaacg ttaacagcgc ctggcgcttc
     2101 tccgggtccg tctccctctc ggccttctca tacgccagca gaatcgcacg tcgaatcgcg
     2161 gtcgcctgca caatcgattt gagtccggga gcgaattgcg cccagtcgtt atgaccaaag
     2221 tagctgtcac gagcgccggt cgccaggatc aagtagtcat aggggataga ttgatcctgc
     2281 atgagcacaa gcttcgcctc tacatctacg cctgtcacct ctcccagcac cacgtccgcg
     2341 tttttttgct tcctgagtat gctgcgaatg ggtgcgcaga tatcagcagg ggagatcccg
     2401 gctgtcgcaa cctggtacag gaggggttga aacaggtgat ggttattgcg gtcaatcacc
     2461 gtcacccgga cgggcgcgtc tcgcaaggct cttgctgcct gtagtccacc aaagccggcc
     2521 ccaacgatca cgacatgggg aatgaccgca ggaacacgag atgcaacgcc cggtagtgtg
     2581 accgattgct catcttcaat gtatgtttct ggtatctctt gcatgttctt tacctcttcc
     2641 tttacagtca tcttgctcca tacgtttctc cctttctatt cgttgatcat gcacgaaatt
     2701 gagcgattgt ctttaacaac cgcgttgtac agcacacacc tcctcagcga ttgagcaagt
     2761 gcaagtcgta ggcggcccgc tccagctgtt cgcggaactg gggcgccgca atgctgatca
     2821 gcaagcgagc ccggtcagcg gtactgcgtc cgaacagcat gaccctgcca tactcggtgg
     2881 ccacatagtg gacgtgcgcc cgtgtggtgg tcacgaccgc cccgggcgtc agcgtgggca
     2941 cgatgcgcga gacctgtcca tcaatcgacg ggagctcgta cggcatgttg ccctccctgg
     3001 cctggggtgc gcgagccgtc gcggggaacg ctagaaccgg tcgcccgtgc ggagcgtcgg
     3061 cggcgccctg gagaaaatct atttgtccgc ccaccccact atactgggcg gtgccaatcg
     3121 actcggcaca tacctgcccg gtcagatcta cctccagggc gctattgatg gccaccatat
     3181 ggtcaaattg acgaatgacc tcgatatcgt tggtgtagct gatcgaacgc agctcaacca
     3241 tgggattatc atccagaaag tccatcaggc gacgcgaccc tatgataaag ctggcgacta
     3301 tcttgccgcg gtcaagggcc ttgcgggacc cggtaatgac tccccgctcg acgaggtcaa
     3361 gcactccatc ggagaacatt tcgctatgga tgcccagatc acgccgatcg gttaattcct
     3421 ggaggacggc gtccggaatg gcgccgatac caagttgcag cgtggcccca tcctcgatca
     3481 gatccgctac atgccggcct atctcccgct gcatc
//
LOCUS       DASZAK010000175         4049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_287790, whole genome shotgun sequence.
ACCESSION   DASZAK010000175 DASZAK010000000
VERSION     DASZAK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287790"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            210..911
                     /locus_tag="VGT44_02950"
     CDS             210..911
                     /locus_tag="VGT44_02950"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV2579787.1"
                     /translation="MQTNGVSITWLGHATFKITSPDGKVVLIDPWVNNPVCPTEQKNI
                     DRVDLMLVSHGHFDHVADAVSIAQRLKPTVAAMVELAGWLQSKGVENTIGFNKGGTIT
                     VNGIKVSMTHAIHSGSTEDGTYGGDPVGFVIEFENGFKLYHAGDTCAFSDMAIIGELY
                     RPDVALLPIGDFYTMGPREAAIAVRMLGVKQVIPMHYGTFPALIGTPAALSDALHSLG
                     LQDVEVVEIKPGQTI"
     gene            1060..2238
                     /locus_tag="VGT44_02955"
     CDS             1060..2238
                     /locus_tag="VGT44_02955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2579788.1"
                     /translation="MSETADILIIGGGVMGASIAYHLAKERQGKVVLLERQAICTGTT
                     GRSGAIVRQHYSNEFSIRMAMKGLTIFQHFDALVGGDCGFVTTGMIVMIDEQGADALR
                     ANVQMQQEQGVDTCLLEPGEVAEAAPGYSSEGVALACYEPDAGVADPMATTTSLAQRA
                     REYGATIYEGTLVTSILTDGERVTGVETTRGRVDAPIVTLAANVWSAGLASPLGITLP
                     ITATRHPMIALRRPNDYGGRLGMHAVGLDLMRDIYLRPDLGGITLVGSSQNVFKPSNP
                     DDYAQGLSEAEIAHFRARANGSIPALARAVPRGGWAGIYDDTPDFHPILDRLAPYDGL
                     YCAAGFSGHGFKLSPVIGQWMAHYILTGQKPDDMHHFAYDRFARGAEIRPRYSSGVLG
                     "
     gene            2393..2965
                     /locus_tag="VGT44_02960"
     CDS             2393..2965
                     /locus_tag="VGT44_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV2579789.1"
                     /translation="MRSDIVPGAKFPDYELTDHTRTRRRLSELQGTDPMILILSRGHY
                     CPKDHQQHLELARFYPQIAVSYTKIVTISTDNILETNEFRDAVGAQWTFLSDSGRKVQ
                     EDLDIQEYTDPFHNPMIPHTIVLEPGLVIYKIYNGYWFWGRPSPEDLRNDLRTIFQRI
                     RPDWDLSAPGLRENWESGDKAMHYPYKRPS"
     gene            complement(3009..>4049)
                     /locus_tag="VGT44_02965"
     CDS             complement(3009..>4049)
                     /locus_tag="VGT44_02965"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV2579790.1"
                     /translation="SKRKRATPAQDALSTGQAVADVAPPIGTTPPRVLARDAADGRRG
                     AAWRLLVWVIENDVRAVEAVSSLDDDRLAQNLLEFMALGTWAGKSFVIPVPLRTAYAR
                     TRLRTLFLPGSGMEYARANRVLLAHIHDSQPAMRETTMNILGIIGDPSATGVLIEALR
                     DPVPAVRLQAAKALGRVGDPAAVPALMGALRGADEQLSSQIFSSLVKLGGMAVPALLQ
                     KSKSNTAWIRWNSIRALSEIHDLRVLPALVEALQDNDHSVAWMGAKGLAHYGKLAMQP
                     VLKMLMTSQMTPWLAETASYVLRNYAQRDADAKPILEPVVLRMREAGFRVGTAVAAQK
                     ALDQLRDGGLLA"
BASE COUNT          878 a         1198 c         1166 g          807 t
ORIGIN      
        1 atgtctccag gagaagcgta ccacgcgctt ttttaacctg tcaagggaaa cctgcggcac
       61 gccgatggag ctaagctttt gttagtatta taattcggat aacaccgagc gtggagagaa
      121 tattccttgc atttccctct cctgcctgat actctgttct cataaagagt gctgcaaagt
      181 acctatcatt tttcaaggag gaaccaacca tgcaaaccaa tggagtaagc attacctggc
      241 tgggacacgc tacattcaaa atcaccagcc cggatggcaa agttgttctg atcgatccgt
      301 gggtgaataa tccggtttgc cctacggaac agaaaaacat cgacagggtt gacctgatgt
      361 tagtttccca tggccacttc gatcatgttg ccgacgcggt gagtattgct caaaggttga
      421 aaccaacggt ggccgctatg gtggaactgg cgggctggct ccagtcaaag ggcgtcgaaa
      481 atacgattgg gttcaacaag ggtggaacca tcaccgtcaa tggcatcaag gtcagcatga
      541 cgcacgccat ccattcaggc agcaccgagg atgggacgta tggaggcgac cccgtcggtt
      601 tcgtcattga attcgaaaac ggcttcaagc tctatcacgc cggagacact tgcgccttca
      661 gtgatatggc gattattggc gaactctacc ggcccgatgt cgccttgttg cccatcggcg
      721 acttttacac tatgggaccg cgcgaagcag ccatagcggt gcgaatgctg ggagtcaagc
      781 aagtcattcc tatgcactac ggcaccttcc cggccctgat cggcacaccc gcagcgctca
      841 gcgatgccct gcattcactt ggtctgcaag atgtcgaagt agtcgagata aagccgggcc
      901 aaaccatcta aacagcgatg cctctggtga gtgttccacc cgtttgtcta ccaggagaca
      961 attagcacag ctaaacggat gagataccac caggcatgcg tcctgctcat gctgccgcag
     1021 ctaccgctat gctggcagga agaaaggaga gcgtaccgcg tgtccgagac agcagatatc
     1081 ctcatcattg gtggtggcgt catgggcgct agcattgcgt atcacctggc aaaagagaga
     1141 caggggaagg tcgttttgct ggaacggcag gctatttgca ctggcacgac tggccggtcg
     1201 ggggcaatcg tgcggcagca ttattccaac gagttttcga tccgcatggc gatgaagggg
     1261 ctgaccatct tccagcattt cgatgcgctg gttggcggag attgcggttt cgtgacgacc
     1321 ggcatgatcg tcatgatcga tgagcagggg gcggacgcgc tgcgcgccaa cgtgcaaatg
     1381 cagcaggagc agggcgtcga tacctgcctg cttgagccgg gtgaggtcgc cgaggccgct
     1441 ccgggctata gcagcgaagg cgtggcgctg gcctgctacg agccggacgc gggcgtggcg
     1501 gaccccatgg cgacgaccac cagtttagcg cagcgcgccc gcgaatacgg cgcgacgatc
     1561 tacgaaggca cactcgtcac cagcattttg accgacggcg agcgcgttac aggtgttgag
     1621 acgacgcgag ggagggtaga cgcccccatt gtgacgctgg cggcgaatgt ctggagcgcc
     1681 gggctggcct caccgctcgg catcacactg cccatcacgg ccacgcgcca cccgatgata
     1741 gcgctgcgcc gacccaacga ctacggcggt cgcctgggaa tgcacgcggt cgggctggac
     1801 ctgatgcgcg atatttacct gcggcccgat cttggcggca tcacactggt cggctcatcg
     1861 cagaatgtct tcaagccgag caaccccgac gactacgccc aggggctgag cgaggccgag
     1921 attgcgcatt tccgcgccag ggcgaacggc tccattccgg ccctggcgcg cgcggtgcct
     1981 cgcggcggct gggcgggcat ctacgacgat acgcccgact ttcaccccat cctcgaccgg
     2041 ctggctcctt acgacggtct ctattgcgcc gccgggttca gcgggcatgg cttcaagctc
     2101 tccccagtga tcgggcagtg gatggcgcac tatatcctca ccggtcaaaa gcccgacgat
     2161 atgcaccatt ttgcctatga tcgtttcgca cgaggagcgg agattcggcc gcgctactct
     2221 tcgggagtgc tgggctaaag tcacacgaag aaactataga ggaaatatcg atgacgtcct
     2281 gaatgttatg taataaagct agaatgtcca cctgaaaaaa cattagccct atcatccttg
     2341 gctacggtcg ggatgacagg gccgaacttg aaaaaaggag atcctttacg acatgcgttc
     2401 agatatcgtt cccggagcaa agttccccga ctacgaattg accgaccata cacgcacacg
     2461 acgtcgcctc agcgaattgc agggcaccga tcccatgatc ctcatccttt cgcgcggcca
     2521 ctactgtcca aaagatcacc agcagcacct ggaactggcc aggttttatc cccagattgc
     2581 cgtctcttac accaaaatcg tcacgatttc gaccgataac atcctcgaaa cgaatgagtt
     2641 ccgcgatgca gtaggggcac agtggacgtt cctctctgat tcgggacgca aggtgcagga
     2701 ggacctggac atacaggagt ataccgaccc gttccacaac cctatgattc cacatacaat
     2761 cgtccttgag ccagggctgg taatctacaa aatctacaac ggctactggt tctggggtcg
     2821 cccatcgccg gaggatctcc ggaacgatct gcggaccatt tttcaacgga ttcgcccgga
     2881 ttgggacctc tccgcacctg gattgcgcga aaactgggag agcggcgaca aagcgatgca
     2941 ttacccctac aagagaccat cgtagcaatc atctgtgggg taatgcatcg ctttgtttcg
     3001 agttaatatc acgccagcag cccgccatct ctgagttgat ccagcgcctt ctgtgcggct
     3061 accgccgttc ccacgcggaa gcccgcctcg cgcattcgca gcacgaccgg ctccaggatg
     3121 ggtttcgcat cggcatcgcg ctgcgcgtag tttcgcagca catacgatgc cgtctccgcc
     3181 agccagggcg tcatctggct ggtcatgagc attttcagca cgggctgcat ggcaagtttg
     3241 ccatagtgcg caagaccctt cgcgcccatc caggccacag aatggtcgtt gtcttgcagt
     3301 gcctcaacca gcgcgggcag cacacgcagg tcatgaattt cgctcagggc gcggatgctg
     3361 ttccagcgta tccaggcggt gttgctcttg ctcttctgga gcagcgcggg aaccgccatg
     3421 ccgccaagtt tgaccagcga agagaagatc tggctgctta gctgctcgtc ggcgccgcgt
     3481 aaggccccca tcagcgcggg aacagcggca gggtcgccta cgcgccccag cgcttttgcg
     3541 gcctgcaagc gcaccgccgg aacaggatcg cgcagcgcct cgatcaagac acccgtcgcg
     3601 gaaggatcgc cgataatgcc gaggatattc atggtagtct cgcgcatggc tggctgggaa
     3661 tcatgtatat gggcaagcaa tacgcggttg gcgcgcgcgt actccatgcc cgaaccaggc
     3721 aagaagagcg tgcgcaggcg cgtcctggca tacgcagtgc gcagcggcac cggtatcacg
     3781 aacgatttcc cggcccacgt ccccagcgcc ataaattcca gcaggttttg cgccaggcgg
     3841 tcatcatcga gggaagagac agcctcgacc gcgcgcacat cgttttcaat gacccatacc
     3901 agtaaccgcc atgccgcgcc gcgccgaccg tctgccgcgt ctcgcgccag caccctcggc
     3961 ggtgttgtgc caatcggagg cgcaacgtcc gcgactgcct gtccggtgga tagagcatct
     4021 tgagcaggtg tggctcgttt gcgcttcga
//
LOCUS       DASZAK010000176         4477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_217477, whole genome shotgun sequence.
ACCESSION   DASZAK010000176 DASZAK010000000
VERSION     DASZAK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217477"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1541)
                     /locus_tag="VGT44_02970"
     CDS             complement(54..1541)
                     /locus_tag="VGT44_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV2579791.1"
                     /translation="MMRMTLPHEKRSGPLTNIRRVKGKPGDRYEVIFLDQHGKLVVPL
                     TEWYRLRTERGPSSTRNTYLACLLPFFSFIAEKQCRWNAPPEQLRPALLEFHRDRLGC
                     LIRPTKDQAGVAMLPTRDTPLCNSTLRVMRAALRDFYLVMKEERLYLFPNPLSSEVLV
                     ALKHAQVQALANRGAPDRAGIREESHEQSRRRPTAFIRYSNTKAWKPSLRKELADVRE
                     GMHTVLNALIDSKDVPLREKAVLELLQNTGARLHEVVQMTVGGYRNEGIAGQAQVVNK
                     GSYGSECKTVYFAHNPHVQQALTAYIEHVRPLHDPQRRTTFAELPDSEPLFLTVRGTP
                     YSVKGFYYHWYKHYPPLAHQCPVRFSPHDIRHLFISEYLIKLRQLCGAGTGHFDAEQY
                     RREREAFGIMVMGWSSARTIDIYDHSRDGEGSLHALAVLQQDFAQRRVVSEPSPLEDP
                     AVAQESMGASDTETATLVREGEVVWLHDPETLAWIKRMQQQTDQQ"
     gene            complement(1868..3400)
                     /locus_tag="VGT44_02975"
     CDS             complement(1868..3400)
                     /locus_tag="VGT44_02975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEV2579792.1"
                     /translation="MLAKIGSCAVTGLEGALVEVEVDLSTGPAAFTIVGLLDAAVNES
                     KERVRSAIKNSGCLFPFKRTTVRLAPAALRKDGPAIDLAIAIGILLASGQIGPDDRVG
                     ECLFLGELSVDGSLRHTNGILPMAALAAERQVATIFVPAADAMEAALVRGVTLYPVET
                     LGQLVAHLNGERLIEPYLPDPRALSAVDHVSYEQDMAAVRGQEHVKRALEVAASGGHH
                     ILMSGPPGSGKTLLARSVPSILPRMTIEEALDVTKIYSVSGMLPQDMPLVLQRPFRAP
                     HHTISPAGLVGGGRIPRPGEISLAHRGVLFLNDLPEFGQNVLEVMRQPLEDKVVAISR
                     AQGTISYPANFMLVAAQRPCPCGRYGDPVKECTCSETAIVRYHKRISSPLLDHIDIHV
                     EVPRVDYEKLADRRSIENSTTIRARVQAARERQLQRFAGTRLTCNAEMGPPEVRYFCA
                     VEPAAEKLLQAAMQQLHLPARAYQRVLKLARTIADLAGSEGIAANHVAEAIQYRPRVG
                     LS"
     gene            3836..3964
                     /locus_tag="VGT44_02980"
     CDS             3836..3964
                     /locus_tag="VGT44_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579793.1"
                     /translation="MHLERIYKPDIECQLRAIVLILGIRVRHAPQKKQEGRDSKKM"
     gene            4061..>4477
                     /locus_tag="VGT44_02985"
     CDS             4061..>4477
                     /locus_tag="VGT44_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV2579794.1"
                     /translation="MENHEQATGSTPSRRALLYLRSAAYNEGEIARQAEGCQRYLSQC
                     TYTYIASIVDNGCSGIGREARPGLVQILEMCEAGAIEALICFRLDRLSRDPVELLELV
                     TRLKQAGIRLETVEEGDIFAQPDTLHGLEFHARCLRH"
BASE COUNT          972 a         1300 c         1273 g          932 t
ORIGIN      
        1 atctctgttg ctgtcgtatg cgtcttcgtt gtcatctggt cttctccttg aagctactgt
       61 tgatctgttt gctgctgcat ccttttgatc caggcgagtg tctcaggatc atgcagccac
      121 accacttctc cctcccggac gagggtagcc gtttctgtgt ctgaagcgcc catcgattcc
      181 tgggcgacgg cgggatcctc gagaggagaa ggctcagaca caactcgtcg ctgtgcgaaa
      241 tcctgttgaa gcacagcaag ggcatgcaaa gatccttcgc catcccgaga atggtcgtag
      301 atatcgatgg ttctggcgct actccagccc atcaccatga tgccaaaagc ttcacgttct
      361 cgccggtact gctctgcatc aaaatggcct gtccccgctc cacacagctg acgaagcttg
      421 atgagatatt cggaaataaa caaatgccga atatcatgcg gggaaaatcg caccggacac
      481 tggtgagcca atggtgggta gtgtttatac cagtggtagt aaaagccttt gaccgagtag
      541 ggtgtccctc gcaccgtgag aaacagcggc tcactgtccg ggagctcggc gaatgtcgtc
      601 cgcctttgtg gatcgtgcaa cgggcgcacg tgctcgatat aggctgtcaa tgcctgctgc
      661 acatgagggt tatgggcaaa gtaaacggtt ttgcattcgc tgccgtagct gcccttgttc
      721 acgacctggg cttgtccagc aataccctcg ttccgatacc cacctacggt catctgcaca
      781 acttcgtgaa gcctggctcc ggtattctgg agcaactcca gcaccgcttt ttccctgaga
      841 ggcacgtcct tgctatcgat cagcgcattc agtaccgtat gcattccttc gcgcacatca
      901 gcgagctcct tccgcaaact gggcttccat gctttggtgt tcgagtagcg gatgaatgcc
      961 gtgggacgcc gccgcgactg ttcgtgcgat tcttcccgaa ttcccgcccg gtccggagct
     1021 ccacgattgg ccagagcctg gacctgtgca tgtttgagcg ccaccagcac ttccgaggag
     1081 agcgggttgg ggaaaaggta caatcgttct tctttcatca ccaggtagaa atcgcgcagt
     1141 gccgcgcgca tcacgcgtag cgtgctgtta cagagcggag tgtcacgggt cggaagcata
     1201 gcaacgccgg cttgatcttt cgttgggcgg atgagacatc ccagtcgatc acgatgaaat
     1261 tcgagaagcg ctgggcgcaa ttgctcagga ggggcattcc agcgacactg cttctcagca
     1321 atgaatgaaa aaaacgggag caggcaggcc aggtaggtgt tgcgtgtact gctgggtccc
     1381 cgttcggtac gtaaccggta ccactccgta agcggaacaa caagcttgcc atgctggtcg
     1441 agaaagatca cctcgtagcg atcacctggt ttccctttga cccgacgaat gttcgttagt
     1501 ggaccagacc tcttctcatg tggaagtgtc atgcgcatca tccccctttc ctgaaaacgt
     1561 gcgatggttc cttatcacag gaaacgtcta actggcatgg tttggtcgcc tccctggata
     1621 gtctgttgta aacagatgtg ggaaaagggt actgttggcc tcctcgccag ctcttctcaa
     1681 tgagaatggg taactagcac taacttgtcg cggacagtac aaggagtcat aggttaggct
     1741 tgcgggtagc cagggcgact tcaagcgaga gcgagttctt caaagcgccc ttcagaaatg
     1801 cggcgagagg agcatacact accactgggg aaaaggaata catccactcg ccgcacccgg
     1861 cgaagcacta cgacaggccg acccgcggtc ggtactggat ggcttccgct acgtgattgg
     1921 ctgcaatgcc ctcgcttccc gccaggtcgg caatcgtgcg ggccagcttc aagacgcgct
     1981 gataggcgcg ggccggcagg tgcagctgct gcatggccgc ttgcagcagt ttctcagcag
     2041 ccggttccac tgcacagaag taacgaactt cagggggtcc catctcggca ttgcatgtta
     2101 accgcgttcc ggcgaaccgc tgcagttgtc gttcccgcgc agcctggacg cgagcgcgaa
     2161 tggtggtcga gttctcaatg ctgcgccggt ccgccagttt ctcataatcg acgcgtggca
     2221 cctcgacatg gatatcgata tggtcgagca aggggctact gatgcgcttg tgatagcgca
     2281 cgatggcagt ctccgaacag gtgcattcct tgaccgggtc gccatagcgg ccacatgggc
     2341 agggacgttg ggccgccacc agcatgaaat tggcgggata ggaaatcgtt ccctgcgcgc
     2401 gcgagatggc gacgacttta tcctccagag gctggcgcat gacctccagc acattctgcc
     2461 caaattcggg caaatcattt aagaataaca ccccgcgatg cgccaggcta atttctcccg
     2521 ggcgcgggat gcgcccgccg ccgaccagac cggcagggct gatcgtatgg tgcggagcgc
     2581 gaaacggccg ctgcagcacc aacggcatat cctggggaag catcccgctg acgctataga
     2641 tcttcgtgac atcgagagcc tcctcaatcg tcatacgagg aagaatcgag ggcacagacc
     2701 gggccagcag ggtctttcca gagccgggag gcccgctcat caaaatatga tgccctccgc
     2761 tcgccgcgac ctccaatgcc cgtttgacgt gctcctgccc gcgcaccgcc gccatatcct
     2821 gttcatacga cacgtgatcg actgcgctga gcgcacgagg gtctggcagg tagggctcga
     2881 tcagccgctc gccgttgagg tgagcaacca gctggcccag cgtctcgacc gggtaaagcg
     2941 tgacaccccg taccagggcg gcctccatgg catctgccgc cggcacgaag atagtcgcga
     3001 cctgcctctc cgccgccagg gctgccattg gaaggatgcc gttcgtatgg cgcaaactgc
     3061 cgtctacaga aagttcgccg aggaaaaggc actcccccac ccgatcgtca gggccgatct
     3121 gcccagacgc cagcagtatg ccgatggcta tggcaagatc gatagccggg ccatctttcc
     3181 gcagagcagc gggcgcgagc ctgacggtcg tgcgtttgaa agggaaaagg cagccgctgt
     3241 tcttgatcgc cgaacgtact cgttctttgg attcattgac ggcggcatcg agaagtccaa
     3301 caatcgtgaa agcggcgggg ccagtggaca gatcaacttc aacctcaacc agcgccccct
     3361 ccagcccggt gaccgcgcag ctccctattt tggccaacat agacgacacg cctcctcaac
     3421 atctttacgc gcattatgca ggaggtgaga gaaaaatgca agcagttcga tcggcccgca
     3481 agcccctggg ggtgatgggc ttccctcgat cgtcttatcg tcttcccccg gttgtcggaa
     3541 tcacctgcct ctcgatactg cttcctctct ggaacttcct cgattcacct tttagccttc
     3601 cctcgatcgc ccgaatcgcc tgccacctat ctccgcctcc accaagaaat gcaggtcggc
     3661 cactcggtcg ctctacatgg gtagaaagaa gcagcggcag ccaatcggca tatcatcggc
     3721 gggtcgaaag cgaatcgaga gcggccagac ggcaagtcga gggcagttgg tcccagctat
     3781 gattgctgat gataaaaacg tcttgccatg atctctggag aaagggagta ttgaaatgca
     3841 tcttgaacgc atctacaagc cggatattga atgccagctg cgggccattg tcctgatcct
     3901 gggcattcga gtccggcacg cgccgcaaaa gaaacaggag ggaagagatt ctaaaaaaat
     3961 gtgaagaccg acctttttgc tttggcgaac gttacttgtt aagtgggcca atgctcagga
     4021 atgggcaacg gcgtgagtac aaaagaaagg aatagagacg atggaaaatc atgaacaggc
     4081 aacgggcagc accccatcca gaagagcgct gctctacctg cgcagtgccg cctacaacga
     4141 aggggagata gcccgccagg cagaaggctg ccagcgttac ctgtcccaat gcacctacac
     4201 ctacatcgct tcaatcgttg ataacggctg tagcggcatc ggtcgggagg cgcggccagg
     4261 ccttgtccag atactggaga tgtgcgaagc gggggcgatc gaggcgctca tctgcttcag
     4321 gctggaccgt ctttcgcgcg accccgttga gcttctggag ctggttaccc gcctcaaaca
     4381 ggcaggcatc cggctggaga ccgtagaaga aggcgacatc tttgcccaac cggacaccct
     4441 gcatggcctt gagttccacg ctagatgttt gcggcac
//
LOCUS       DASZAK010000177         5470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_287816, whole genome shotgun sequence.
ACCESSION   DASZAK010000177 DASZAK010000000
VERSION     DASZAK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287816"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VGT44_02990"
     CDS             complement(<1..228)
                     /locus_tag="VGT44_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579795.1"
                     /translation="MAQFMNFPTARERVLTSGDTTIGIIPDICLVSHFQVAAWQVLYR
                     PQETGNVKRWGMPLMIPNFSRLKDGIFLEKGT"
     gene            452..1255
                     /locus_tag="VGT44_02995"
     CDS             452..1255
                     /locus_tag="VGT44_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579796.1"
                     /translation="MSKEPETTTTPTTQTETVTKQQSWRRWSRLAMLAFTLVGPIINS
                     TRERLRRQAETTTNALAENAQSRQVDLQELRERFDALTEESRERVAQQAQFLRTQARQ
                     LKTQSRQLRKAIRKEARQRQKWLKQAREAGVDWSQEMLKRGEQFSDEVIERGGKVTHD
                     LVERGQGLSHDLAKRGRKVTRSVARRGEELFEPTRERSRVWAVVGFGVGMIVAGAITY
                     RFVRGRSALMQAENESIELEPPTSMNGNVPVAVNADGEEAEQPGHTTAE"
     gene            complement(1351..1785)
                     /locus_tag="VGT44_03000"
     CDS             complement(1351..1785)
                     /locus_tag="VGT44_03000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579797.1"
                     /translation="MITQRIKNWLRQVFSWWPSRRQASPVEYRHDSGALSVVAPSEGV
                     SLSALEGTTPQTSATNTTGAATPFLSTTEERPAYTALTPSPANDEPAAPHLPGSPADI
                     VPASGEAVALTAHIPPAPTPQQRLEFLRYLVKRGIVNEGKEE"
     gene            2013..2225
                     /locus_tag="VGT44_03005"
     CDS             2013..2225
                     /locus_tag="VGT44_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579798.1"
                     /translation="MSVMQEDVLGTRRTVISDPLVVCSRCNKVTRLRFARLVQSDVLS
                     DSHSEYEYLCEECQKALADGEKDLAL"
     gene            2404..5157
                     /gene="lon"
                     /locus_tag="VGT44_03010"
     CDS             2404..5157
                     /gene="lon"
                     /locus_tag="VGT44_03010"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV2579799.1"
                     /translation="MASKKKDIQDQQVPTSKVEETQRGVGEEETSEEQKVPVKRAPRP
                     RAKKQVDESQEAAPVAKEPEVPVQKPRRRRRKRDEDDELLIPGIMPVLPLKDVVVYPF
                     AMQPLGVGQARSIRLIDDIMRSNRLVVMVAQKSAEIESAGPKDIFRVGTVARIARMIR
                     IPDGTLQIIVQGLERVEIGEFVQEKPYLTAHISAKPEIQETDDETEAIKRNVVGYFQR
                     LVTLVQNVREELATTALNMEEARQVAYLVATFLQMDLSLRQQVLELDSVRAKLETLSA
                     YLSHELEIFELGRKIQTSAQEEMSKVQREYLLREQLKAIQRELGEESEEQATINELRR
                     KLDEARLPEEALKEANRELSRLEKLPSASPEYSIIRTYIELLASLPWSKSTGEQIDVP
                     HAREVLDQDHYDLEKIKDRVVEYLAVRRLKEERLAEQLKLEQEALESLEEERRPTQPL
                     SAAEHRRQVNREPILCFVGPPGVGKTSLGQSIARALGRKFARMSLGGIRDEAEIRGHR
                     RTYIGAMPGRIIQTLRRVETNDPVIMLDEVDKVGADWRGDPSSALLEVLDPEQNYNFR
                     DNYLDLAFDLSRVMFIATANSLEPIPPALRDRMEILELSGYTEEQKLHIARNYLLSKQ
                     LEANGLKADELVVDDDALRRIARDYTREAGVRNLEREIGTLCRKIAKQIAEGKATPIT
                     VSAETLTDYLGRQRFFEEVAERIDRPGIATGLSVTPVGGEILFIEAASIPGKEDQLTL
                     TGQLGDVMKESAMTALSYVRSNAALLGLPPQVFEHQHVHIHVPAGSIPKDGPSAGVTM
                     ASVLVSLASGRKVRSDVAMTGEITLRGKVMPIGGVKEKVLAAYRSGIRTVILPKKNEP
                     DLMEDLPKELRDQMHFVFASEVRQVLETAIEGFPQIAASENGHQRPRRRRGEQAAAAS
                     VQA"
     gene            complement(5165..>5470)
                     /locus_tag="VGT44_03015"
     CDS             complement(5165..>5470)
                     /locus_tag="VGT44_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579800.1"
                     /translation="VSGKRNRKRGTTMLILSLLWVALGLLIGALANAARLRPATWGRG
                     GWPSMLGLGALVALLGGWLGTWLFGALFATATALWIAVLGVALLPRLIMWCYRFLRP"
BASE COUNT         1238 a         1548 c         1682 g         1002 t
ORIGIN      
        1 ggtgcctttc tccagaaata tgccatcttt caagcgcgag aaatttggga tcatcaatgg
       61 cataccccag cgtttgacat tgcccgtctc ctgcgggcgg tagagtacct gccacgccgc
      121 tacctggaaa tgcgatacca ggcagatgtc aggaatgatg ccgatggtgg tatcgccgct
      181 ggtcaagacg cgctcgcgcg cggtcgggaa gttcatgaac tgcgccattg tctcgtcctc
      241 ttttcgggtg atgtcacatt tattgtacgt gtgtcgcctg gtgaccgtca atccgtgttt
      301 ccgccttgac atacactcgc gcaagctgac atactactat ttgcgtgtgt gaaatcagtt
      361 aaaatggaaa agaagggcgc tttgtgcgcc tttgagtgag atgtagagca gattccaccc
      421 tgtggagtat tttagtaggg gtacaaacaa catgagcaaa gaaccagaga caacaaccac
      481 accgactaca caaacggaga cagtgacaaa gcagcagagt tggcgacgct ggtcgagatt
      541 ggccatgctg gcatttacgc tggtcggccc cattatcaac tcgacgcgcg aacgcttgcg
      601 caggcaggca gagacgacca cgaacgcgct ggctgaaaac gcgcaatcac gccaggttga
      661 cttgcaggaa ctgcgcgaac gttttgatgc gctgacggaa gaaagtcgcg agcgagtggc
      721 acagcaggcc caatttttgc gtacccaggc gcgccagttg aaaacgcaat cgcgccagtt
      781 gcgcaaggcc attcgtaagg aggcccggca gcgccaaaaa tggttgaagc aggcacgtga
      841 ggctggcgtg gattggagcc aggagatgtt gaaacgcgga gagcaattct ccgacgaggt
      901 gatcgaacgc ggcggcaaag tcacacacga cctggtagaa cgcggccagg gactgtcgca
      961 cgacctggcg aaacgcggtc gcaaagtcac gcgcagcgtc gccaggcgcg gcgaagaatt
     1021 gtttgagccg acgcgcgaac gcagccgcgt ctgggcagtc gtcggcttcg gtgtaggcat
     1081 gatcgtggca ggcgcgataa cctaccggtt tgtgcgcgga cggtcagcgc ttatgcaagc
     1141 cgagaacgag tcaatcgaac tcgaaccgcc tacttcgatg aacggcaacg ttcctgtcgc
     1201 cgtgaatgcg gatggggaag aagccgaaca accggggcat acaacagccg agtaagagac
     1261 aaagaaactg ctcacgatgg ccagggccgt tgaaaagtcg gggcatggcg accgcggtcc
     1321 ctcgcggtcg ccatgctgtt tttcgctcgc ttattcctct ttgccttcgt tcacgatacc
     1381 tcgtttgact agatagcgta agaattcgag gcgctgctgt ggcgtgggag ctggtggaat
     1441 atgcgccgtg agcgccactg cctcccctga tgccgggacg atatcggctg gtgaaccggg
     1501 taggtgaggt gctgccggtt cgtcgttggc gggtgatggg gttagcgctg tatatgcggg
     1561 gcgttcctcg gtagtagaga gaaacggcgt tgcggctcct gtagtgtttg tcgcgcttgt
     1621 ttgcggcgtt gttccctcaa gcgccgagag cgacacgccc tctgaaggcg cgaccacgct
     1681 caacgcgccg ctatcatgac gatactcaac cggcgaagcc tgccgccggg atggccacca
     1741 ggagaaaacc tggcgcagcc agttttttat ccgttgcgtg atcatcgtgt ccttgctttc
     1801 ggtcctatgg ctagcaatca cgcgctacat gagcaacaca acaaccatga gtaatcatga
     1861 atggtataga aacatagtag cacatgctgt ccattgcttg caagtgaata tgcacacttg
     1921 catttttcgc ctctgcgtgc cataatcaaa tgggttccga tggattaccc cctctgaaag
     1981 atagtcacta ctgaaacaga aaagaaaggt aggtgtcggt tatgcaggaa gatgtgctgg
     2041 gaacccggcg aaccgtcatc tctgatccgc tcgtcgtctg ttctcgttgc aataaagtca
     2101 ctcgattacg atttgcccgt cttgtgcaga gcgatgttct gtcggatagc cattccgagt
     2161 atgaatatct ctgcgaggag tgccagaagg cgctcgctga cggggaaaag gacctggcgc
     2221 tctaaaggcg accatcttac agtttaccgt tttctatggc aaaaggcgcg ggaagttcac
     2281 tcttgtccct tttgccaatt tttcaccctt catcgaccgt tatcattcgc ctgctaatac
     2341 gtgataagaa catgcatgaa gcgtgattct ggaatggata tatcaagaga ttgtgaaata
     2401 acgatggcaa gcaagaaaaa agacatacaa gaccagcagg tcccaacctc aaaagttgaa
     2461 gagactcagc ggggagtggg agaggaagaa acgagcgagg agcagaaggt tccggtaaaa
     2521 cgcgctcccc gcccgcgcgc gaaaaagcag gtggatgaga gccaggaggc tgctccagtt
     2581 gcaaaagaac ctgaagtccc ggtgcaaaag ccacgccgca gacgccgcaa gcgcgatgag
     2641 gacgatgagc tgctcatccc tggcattatg cccgtgctgc cgctcaaaga tgtcgtcgtg
     2701 tacccctttg ccatgcagcc gctgggcgtc ggccaagcgc gctcgattcg cctgatcgac
     2761 gacatcatgc gctcgaaccg gctggtggtg atggtggcgc agaaatcggc ggaaatcgag
     2821 tcggctggcc ccaaagacat cttccgggtc ggcaccgtcg cgcgcattgc gcgtatgatc
     2881 cgtattcccg atggcacgct ccagatcatt gtgcagggtt tggaacgggt agagatcggc
     2941 gagtttgtgc aggagaagcc ctacctgacc gcccatatct ccgccaaacc ggagatacag
     3001 gaaacggacg acgaaaccga ggccatcaag cgcaacgtgg tcggctactt ccagcgcctg
     3061 gtgacgctgg tacagaatgt gcgcgaggaa ctcgccacga ctgcgctcaa catggaggag
     3121 gcgcgccagg tggcgtacct ggtggcgacc ttcttgcaga tggacctcag cttgcgccag
     3181 caagtgctgg agttggattc ggtgcgcgcc aagctggaga cgctcagcgc ctacctctcg
     3241 cacgaactgg agatttttga gctgggccgc aagattcaga ccagcgccca ggaagagatg
     3301 agcaaggtgc agcgcgagta cctgctgcgc gagcaactga aggccatcca gcgcgaactg
     3361 ggtgaggaga gcgaagaaca ggccacgatc aacgaactgc gccgcaagct cgacgaggcg
     3421 cgcttgccgg aggaggcgct gaaagaggcc aaccgcgaac tctcgcggct ggaaaagctg
     3481 ccgtcggcct cgccggagta cagcatcatt cgcacctata tcgaactgct ggcgagcctg
     3541 ccgtggagca agagtacggg cgagcaaatc gatgtgccac acgcccgcga ggtactcgac
     3601 caggatcact atgacctgga gaaaatcaag gatcgggtcg ttgaatacct ggccgtgcgc
     3661 cgcctgaagg aggagcgcct ggccgagcag ctcaagctcg aacaggaagc gctcgaatcg
     3721 ctagaagagg agcggcgacc aacccagccg ctctctgcgg cagaacaccg gcggcaggtc
     3781 aaccgcgaac cgatcctgtg ctttgttggc ccgccgggcg tcggcaaaac ctcgttgggc
     3841 cagtcgatcg cgcgcgcgtt ggggcgcaag tttgcccgca tgtcgctggg cggtattcgc
     3901 gacgaggccg agatacgcgg ccatcgccgc acctatatcg gcgcgatgcc gggccgcatc
     3961 atccagacgc tgcggcgcgt ggagaccaac gacccggtaa tcatgctgga cgaggtggac
     4021 aaggtcgggg ccgactggcg cggcgaccca tcctccgcgc tgctcgaggt acttgacccg
     4081 gagcaaaact acaacttccg cgacaactac ctcgacctgg ccttcgacct gtcgcgggtc
     4141 atgttcatcg cgacggctaa ttcgctcgaa ccgattccgc ccgcgctgcg cgaccgtatg
     4201 gaaatcctgg agctgtccgg ctatacggag gagcagaaac tgcacatcgc gcgcaactac
     4261 ctgctgtcca agcagcttga ggccaacggc ttgaaggcgg atgaactggt cgtcgatgac
     4321 gacgcgctgc ggcggatcgc gcgcgactac acccgcgagg caggcgtgcg caacctggaa
     4381 cgcgagatcg gcacactctg ccgcaagatt gccaaacaga tcgcagaggg caaggcgacg
     4441 cctattacag tttctgccga gacgctgacc gattacctgg gccgccagcg cttcttcgag
     4501 gaggtagcag agcgcatcga ccgccccggc attgctaccg gcctgagcgt gacgcccgtc
     4561 ggcggtgaaa tcctcttcat cgaggcggca tctattccag gcaaagaaga ccagttgacg
     4621 ctgaccggcc agcttggcga tgtcatgaag gaatcggcca tgactgcgct gagctatgtg
     4681 cgctccaacg ccgcgctgct gggcctgccg ccgcaagtct ttgaacatca acatgtgcat
     4741 atccatgttc cggcagggtc gatccccaaa gatggcccat cggcaggtgt aacgatggca
     4801 agcgtactgg tttcgctggc gagcgggcgc aaagtgcgct ccgatgtggc catgacgggc
     4861 gaaatcacgc tgcggggcaa ggtcatgccg attggcggtg tcaaggaaaa ggtgctggcg
     4921 gcctatcgtt cgggtatccg cacggtgatt ctgccgaaga agaacgagcc ggacctgatg
     4981 gaggatttgc ccaaagaact ccgcgaccag atgcattttg tcttcgccag cgaagtccgg
     5041 caggtgctgg agaccgctat cgaaggcttc ccacaaatcg cagccagcga gaacggacac
     5101 cagcgcccac gcaggcgcag gggcgaacag gcggcggctg ccagcgtaca ggcgtaaagg
     5161 ccgctcaggg gcgcaggaag cggtagcacc acatgatcag gcggggcagg agcgccacgc
     5221 ccagcacggc gatccagagg gcggttgctg tggcaaagag cgcgccgaat agccacgtac
     5281 ccagccaccc tccgagcaag gcgaccagcg ctcccagccc gagcatactc ggccagccac
     5341 cgcgacccca cgtcgccggt cgcagcctgg ccgcgtttgc caaagccccg atgagcaggc
     5401 caagcgctac ccaaaggagg gaaaggatca gcattgtcgt tcctcgcttt ctgttgcgct
     5461 ttccactcac
//
LOCUS       DASZAK010000178         3208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_4729, whole genome shotgun sequence.
ACCESSION   DASZAK010000178 DASZAK010000000
VERSION     DASZAK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4729"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGT44_03020"
     CDS             <1..344
                     /locus_tag="VGT44_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEV2579801.1"
                     /translation="AMNYIDAAETLKKWIAALFKGTPEEKPEQYAVSSPISYVEYVSA
                     PILIIQGLNDTRTPARQVRVYEKEMQKYGKLLEVHWFDAGHLAPFAQVEQAVVYQEHM
                     LRFANRILNIV"
     gene            complement(586..2154)
                     /gene="metG"
                     /locus_tag="VGT44_03025"
     CDS             complement(586..2154)
                     /gene="metG"
                     /locus_tag="VGT44_03025"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV2579802.1"
                     /translation="MKEQDKQDSANKRYYITTALDYPNAEPHIGHALEKIAADVVARY
                     HRLLGHDTYLSMGLDENSQHVVQAARDRQVPIPAWIESMDVAFRQALAALDISYDRWM
                     RTTEPAHVRASQEMFRRAQAAGDIYKSTYTGWFCPNCNSFYRDDELVNGACPNHPSRK
                     PDWVEEEDYFFALSAYCDRLLSHITSHPDFIVPVSRQSEIVGIIRQGLRDFPVSRPVN
                     RIDAQWGVPVPGDLDNVIYVWFDALTNYLTAVGFPEGGELFARYWPADAHVIGKDITR
                     FHCLYWPAMLMSAGLPLPRQVAVHGFLTLEGKRISKTAGNVVSPLELVEAYGVDAVRF
                     YLLRHLSFASDGDFSRAGLIQRYNDELAKDLGNLLNRVVSMIQRYRGGQIPATGEPGA
                     LELDVQRAAGEVRQQAGQALESWEIGHALNSVWSFVRRINRYLEERKPWRLAAQQEAA
                     AELDVVLITAAESLRLAAILLAPFLPTAANRMLDQLGLDGMRSSAWYDEGTWGGRALS
                     AVAPGPILFPRIEV"
     gene            complement(2598..>3208)
                     /locus_tag="VGT44_03030"
     CDS             complement(2598..>3208)
                     /locus_tag="VGT44_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579803.1"
                     /translation="QNWVWMHMALGIASVLLALLHADYSFVLHWDCTSQQNCFTAHYW
                     GMPALYALIFIALSGAVGRLLDKWQTRTIAQDASTNSVGIAKAITGRLLELEYVVERF
                     SAGKSEPFKAYCALAIESVGTPPSELPTLAPREQADFQGAYEALKDHARLAQSLQKQH
                     FAHTIFRAWRYAHMVLVPLALVIITYHGIAELLINVLHVIKV"
BASE COUNT          749 a          885 c          898 g          676 t
ORIGIN      
        1 gggccatgaa ttacatagat gctgcagaga cactcaagaa atggatcgca gctctcttta
       61 agggtacacc agaagagaaa ccagaacaat atgccgtcag ttcacctata agctatgtag
      121 agtacgttag cgcacctata ctcatcattc agggactcaa cgatactcga actccggcac
      181 gtcaagtaag agtctacgaa aaggaaatgc agaagtatgg caaattactc gaggtacatt
      241 ggttcgacgc tggccattta gctccatttg cacaggtcga gcaagcggtt gtctatcaag
      301 agcatatgct tcgctttgcc aatcgcatac tgaacatagt atagttttta tttgtctttg
      361 tacatttcca ataaatatac gaataaatag cgtaagccag gcgatcacaa atcgaacgcg
      421 gtattaaaac cactgcggga ttcacagatc ggctttgccc cttgattacg aggcggtatc
      481 ccccgcgacc agatcggtgc cagcggccca cgttcgttca gctattcctc gtagaaggct
      541 tcggatttga tgaacatgtc aggctctcct ttcatcattg cgctcctaca cctcgatgcg
      601 cgggaacagt attggtcccg gcgcaactgc cgacaaagcg cgaccgcccc acgttccttc
      661 gtcataccat gccgatgagc gcattccatc cagcccaagc tggtcaagca tgcggttggc
      721 ggcagtgggg agaaagggcg cgagcaggat ggctgcgagg cgcaggctct ctgccgcggt
      781 aatgagtacg acatcaagct cggctgcggc ttcctgttgc gcggccaggc gccagggttt
      841 gcgctcttcc aggtagcgat tgatgcggcg cacgaaactc cacacactgt tcagggcatg
      901 gccgatttcc caggactcca gggcttgccc ggcctgctgc cgcacctcgc cagctgcgcg
      961 ctgcacatcc agttcaagcg cgccgggttc tccggtcgcg ggaatctgtc cgccgcgata
     1021 gcgctggatc atgctgacca cgcggttgag caggttgccc aggtctttcg ccagttcatc
     1081 gttatagcgc tggatcagcc ctgcgcgcga aaagtcgcca tcggaggcga aggagagatg
     1141 gcgcagcagg taaaagcgca cagcatccac gccgtaggct tctaccagtt cgagcggcga
     1201 gacaacattt ccagcagtct ttgaaatgcg tttgccctcc agggtcagga aaccatgcac
     1261 cgccacctgg cgcggtaggg gcaggcccgc cgacatgagc atggccggcc agtacaggca
     1321 gtggaagcgc gtgatatctt tgccgatcac gtgcgcgtcg gctggccagt agcgcgcgaa
     1381 cagctcgccg ccctcgggaa acccgacagc agtcaggtag ttggtcagcg cgtcgaacca
     1441 gacgtagatg acgttatcca ggtctcctgg cacaggtacg ccccactgcg catcgatgcg
     1501 attgacagga cgcgacacag gaaaatcgcg cagcccctgg cgaatgatcc ctactatttc
     1561 agactgccgc gagacgggta cgataaaatc tggatggctc gtgatgtgcg acagcagtct
     1621 atcacaatac gctgaaaggg cgaaaaagta gtcctcttcc tctacccaat caggcttgcg
     1681 cgaaggatga ttgggacacg cgccgttgac cagctcatca tcacggtaaa agctgttgca
     1741 gttggggcaa aaccagccgg tataggttga tttgtagatg tcgcccgccg cctgcgcgcg
     1801 gcgaaacatc tcctgcgatg cgcgtacatg cgcaggttcc gtggtgcgca tccagcgatc
     1861 ataggagata tccagcgcag ccagggcctg ccggaatgcg acatccatgc tttcaatcca
     1921 cgcgggaatg gggacctggc gatctcgcgc ggcctgcaca acgtgctggc tattctcgtc
     1981 cagccccata ctgagatagg tgtcatgccc cagcaagcgg tgatagcgcg cgacgacatc
     2041 cgccgctatc ttttcaagcg cgtgtccaat atgcggttca gcgttcggat aatcgagggc
     2101 cgttgtgatg tagtaccgtt tgttggctga atcctgtttg tcttgttctt tcatgctgaa
     2161 tactcctctt cactgctata gacgccgtaa agaaaaaaag aaggggcgca ggtctgtcgc
     2221 ctgcgcccct tgtatatgcc tctgccgttt gaccttatcc aggtcaggtc agtaatggag
     2281 atgtacggaa cgaggacagc gacaaccctg cggatgcttc aataacgagc atccggcaaa
     2341 acatggacat aggcatggtg gttagggcat cgtgtcgctc agacatgtgc aatccttttg
     2401 aatgtggatg tatagccaga actatagcta tcactatact tgcaaatttg cgatttgtca
     2461 atgaccggaa tcgtcgtcaa acgagcacat gaacccgttg taggggcgca attcattgcg
     2521 ccctcgcgcc aggccgatgc gtggagcgaa ccagggcgca atgaattgcg cccctacaac
     2581 gggttcatac ggcctcgtca aaccttaatc acgtgcagca cgttgatcag cagttccgcg
     2641 atgccgtgat aggtgataat caccaacgcg agtgggacaa gcaccatatg cgcgtaacgc
     2701 caggcgcgaa agatggtatg cgcaaagtgc tgcttttgga gcgattgtgc caggcgagca
     2761 tgatctttca atgcctcgta agcgccttga aaatcggcct gttctcgcgg cgcaagagtt
     2821 ggaagctcgc tgggaggcgt acccacgctt tcgatagcca gggcgcaata cgctttgaac
     2881 ggctcggact tgcccgcgct gaaacgctcg acaacgtatt ccagttcgag gaggcgaccg
     2941 gtaatggctt tcgcaatgcc aacgctgttg gtactggcat cctgagcaat cgtgcgcgtc
     3001 tgccacttat cgagcaagcg accgactgcg ccgctcagag caatgaagat aagcgcatag
     3061 agggcaggca taccccaata gtgggccgtg aagcaatttt gctgactggt gcaatcccaa
     3121 tgcaggacga agctatagtc ggcatggagc agggcgagca acaccgaagc aatacctaag
     3181 gccatgtgca tccacaccca gttttggg
//
LOCUS       DASZAK010000179         1847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_466110, whole genome shotgun sequence.
ACCESSION   DASZAK010000179 DASZAK010000000
VERSION     DASZAK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466110"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VGT44_03035"
     CDS             <1..515
                     /locus_tag="VGT44_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HEV2579804.1"
                     /translation="IAKWMRLAGVDHIHAGTVVGKLEGDPMSIKGYYQTLRGAKYDAD
                     PSLGLFFEQDWASMPGVMPVASGGIHAGQMDMLLHYLGEDVVLQFGGGTIGHPQGIAA
                     GATANRIAVEAMIQARNEGRDVAKEGQEILEKAARWCGPLRAALELWKDISFNYESTD
                     TPDAVSTPNY"
     gene            532..957
                     /locus_tag="VGT44_03040"
     CDS             532..957
                     /locus_tag="VGT44_03040"
                     /inference="COORDINATES: protein motif:HMM:NF012329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HEV2579805.1"
                     /translation="MPFHLTQGTFSYLPPLTDDEITMQVHYCLNHNWPINVEFTDDAH
                     PRNTYWEMWGLPMFDVMDPAAILSEINECRRAYPHHYIRVNAYDRTKGRQTVALSFIV
                     NRPSIDPGFGLLRQEAHDRVVRYSLHSYATDKPKGERFP"
     gene            954..>1847
                     /gene="cbbX"
                     /locus_tag="VGT44_03045"
     CDS             954..>1847
                     /gene="cbbX"
                     /locus_tag="VGT44_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HEV2579806.1"
                     /translation="MIPPAHTGEAVVEAGTLLESPDDACVDLAAMLQDSQVMEELQKL
                     DDELIGLKSVKTRIREIAALLLVDRIRRSFGLSTRPPTLHMSFTGPPGTGKTTVAMRM
                     ATILNRLGYVRKGHLVAVTRDDLVGQYIGHTAPKTKEVLKRAMGGVLFIDEAYYLYRE
                     ENERDYGQEAIEILLQVMENQREDLVVVLAGYKDRMETFFQSNPGISSRIAHHLDFPD
                     YTLDELLAIAQLMLAEQMYRFSPEAQQAFRRYLERRMTLPRFSNARSVRNAIDRARLR
                     QANRLFARGGKISKIDLISIEA"
BASE COUNT          396 a          570 c          526 g          355 t
ORIGIN      
        1 ttattgcgaa atggatgcgc cttgccggag ttgaccacat tcatgccggg acggtggtcg
       61 gcaagctaga aggcgatccc atgtccatca aagggtacta ccagacgctg cgcggcgcga
      121 aatatgacgc tgacccgtcc cttggcctgt tctttgagca ggattgggcg tccatgccag
      181 gcgtaatgcc ggtcgcctcc ggcgggattc acgctggcca gatggatatg ctgctgcact
      241 acctgggcga ggatgtcgtt ctccagtttg gcggtggcac catcggccac ccacagggca
      301 tcgccgccgg cgcaaccgcg aaccgcatcg ccgtagaggc catgatccag gcgcgcaacg
      361 aggggcgcga tgtcgcgaaa gaagggcagg aaatcctgga gaaggccgcg agatggtgcg
      421 gccccctgcg cgccgcgctt gaactctgga aggatatcag ctttaactac gaatcgaccg
      481 atacaccgga tgccgtttcc acgcctaact actagcgaaa ggggtcctga aatgcctttt
      541 cacctgactc aaggaacatt ttcctatctg ccaccgctga ccgacgacga gattaccatg
      601 caggttcact attgcctgaa tcacaactgg ccgatcaacg tggagttcac cgacgacgcc
      661 catccgcgca acacctactg ggagatgtgg ggcctgccga tgttcgacgt gatggacccg
      721 gcggcgatcc tctccgaaat caacgagtgc cgcagagcct atccccacca ctatatccgc
      781 gtgaatgcct acgaccgcac gaaaggccgc cagacggtcg cgctctcgtt catcgtcaat
      841 cgaccatcga tcgatcctgg ctttggcctg ctgcgccagg aagcgcacga ccgcgttgtg
      901 cggtatagct tacatagcta tgcaaccgac aaaccgaagg gagagcggtt tccatgatcc
      961 cgcctgctca caccggtgaa gcggttgtcg aagccggtac gctgctcgag tcccccgatg
     1021 atgcctgcgt agatctggcg gccatgctcc aggactcgca ggtcatggag gagttgcaga
     1081 agcttgacga tgagctgatc ggcctcaagt cggtcaagac gcgcattcgc gaaattgccg
     1141 ccctgctgct ggttgaccgc atacgacgca gctttggact gtcaacgcga ccgcccacgc
     1201 tgcacatgtc ctttaccggc ccgccgggta ccggcaagac gaccgtagcg atgcgcatgg
     1261 ccaccatcct caaccgactc ggctatgtgc gcaagggaca cctggtggcc gttacacgcg
     1321 acgacctggt cggtcaatac atcggccaca cggcgcctaa aaccaaagag gtgcttaaac
     1381 gcgccatggg gggcgtcctc ttcatcgacg aggcgtacta tctctatcgc gaggagaacg
     1441 agcgcgatta tggacaggag gccatcgaaa tcctgctgca agtgatggag aaccagcgcg
     1501 aagatctcgt cgttgtgctg gccggctaca aagaccgcat ggagaccttt ttccagtcca
     1561 atccgggcat cagttcgcgt atagcacacc acctggattt ccccgattat actctcgacg
     1621 agctacttgc catcgcacag ttaatgctcg ctgagcaaat gtatcgtttc agcccggagg
     1681 cgcagcaagc tttcaggcgc tacctcgagc ggcgcatgac attgcctcgc ttctctaatg
     1741 ctcgcagcgt gcgcaatgcc atcgaccgcg cgcggctgcg ccaggctaac cgcctctttg
     1801 ccagaggagg gaaaatctcg aagatcgatc tcatctccat cgaggcc
//
LOCUS       DASZAK010000180         3819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_324422, whole genome shotgun sequence.
ACCESSION   DASZAK010000180 DASZAK010000000
VERSION     DASZAK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324422"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VGT44_03050"
     CDS             complement(<1..909)
                     /locus_tag="VGT44_03050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2579807.1"
                     /translation="MDFGVLVPQGWRMDLVGIPDPAEAYETMSRVAQEAEALSYHSIW
                     LYDHFHTVPIPTQEVTLECWTSTAALARDTRRVRIGQMVTCNSYRSPALLAKMASTVD
                     ALSHGRLDFGIGAGWYEEEYNAYGYPFASTHERLERLHEAVQVILAMWTQEEARFEGT
                     YYQVRGAINQPKGVQKPHIPLLIGGGGEKVTLRIVAEYADACNLGSDIQTIKHKLAVL
                     KQHCEDLGRDYASIRRTVLIDDCLIAESEAGILAKLTPQERDNLDKLRRSWLIGTPGQ
                     IRERLAQYEEVGIQELIVRFVDAAQLE"
     gene            complement(927..2423)
                     /locus_tag="VGT44_03055"
     CDS             complement(927..2423)
                     /locus_tag="VGT44_03055"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV2579808.1"
                     /translation="MGNVIVGATMSLDWFMNDPFASASTMLAALQTGQVSATELLEQH
                     LQRIERYNPRINAIVIPNYEQARQRAAEADAARARGELLGPLHGLPLTIKDCIEVAGL
                     RTTAGVTHRANAISTQNAPVAQRVLDAGAVLIGKTNVPPYAGDWQANNEVFGRTNNPW
                     DLERSPGGSTGGGAAALAAGLTPLEFGSDIGGSIRIPAAFCGVYGHRPSDSAVPRYGH
                     VPGSPLPNPAFVMGVQGPLARSAADLALALNVIAGPVVGEEVAWHLTLPAARHSALSN
                     FRVAIMPQIPSLPVDAEITAALENLATRLGALGAKVAEAQPESFDWHKYQQTYTSLLD
                     VFMFAELKDEDRAGIVEGLRHSSDPYVQAQLFGLTATASQFLGLLARREHYRAAFRDF
                     FRDWDVLLTPVTITPAFPHISSDLSFPRRTLQINGEVVSYGRIQDYPGLATLSGQPAT
                     AFPWGRTRQGLPIGLQAIGPYLEDFTPIAFASLLEREFGGFVAPPGYA"
     gene            complement(2531..3088)
                     /locus_tag="VGT44_03060"
     CDS             complement(2531..3088)
                     /locus_tag="VGT44_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579809.1"
                     /translation="MQEAVLSPLATWQNFYVIVGTAAATLTGLMFVVIALIAQIRVQV
                     SSPRSGIAVFSTPNVVHFCAALLVAAILNAPWQALWNTAVLLGLGGLGGMAYVLSVLW
                     LARHRLSDYQPVFSDWLWHTVLPLASYTAFVVAAILLPGHAAPALFVIAAATLLLLFI
                     GIHNAWDVVTYTAFERPRQESTSQN"
     gene            3240..>3819
                     /locus_tag="VGT44_03065"
     CDS             3240..>3819
                     /locus_tag="VGT44_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579810.1"
                     /translation="MHRQSFHLSHFRLSTVALSGLLLCLVLLSACGSLTTSSASVAPT
                     PTVNRTLQNKGNAQLQTFQQWITLMQQYQGDVTAYQQQYQGDQQALRSAHSDAAYQKA
                     LNTLDAHVAAIQIPAMKTESGFLFQELQQDATSFGQQHTYHDSYNNTTYPLGYEYGPT
                     GATGPLWLQGELASAQSLADYQQAVDDLNMDLA"
BASE COUNT          820 a         1140 c         1128 g          731 t
ORIGIN      
        1 ttctaactgt gcggcatcaa cgaaacgcac gatgagttcc tgaatgccga cctcttcgta
       61 ttgcgccagg cgttcgcgaa tctgcccagg cgtgccgatc aaccaggacc ggcgcaattt
      121 atccaggtta tctcgctcct ggggagtcag tttggcgaga atacctgcct cgctctccgc
      181 gataaggcaa tcatcgatca ggacggtacg cctgatgctg gcgtaatcgc gcccgagatc
      241 ttcgcagtgc tgcttgagta cggccagttt gtgtttgatg gtttggatat cgctgccgag
      301 attgcaggca tcggcatact ccgcgactat gcggagggtc actttttcgc cgccgccgcc
      361 aatcaggagc gggatatgcg gtttttggac acctttgggc tggttgatcg cgcccctcac
      421 ctggtagtac gtcccctcga agcgcgcttc ctcctgcgtc cacatcgcca ggatgacctg
      481 cacggcctcg tgtaatcgct ccaggcgctc gtgcgtgctg gcaaacgggt agccataggc
      541 attgtattcc tcttcatacc agcctgcgcc aatgccgaaa tcgagccgtc catgactgag
      601 cgcatcgacg gtactggcca tttttgccag gagcgccggg ctgcgataac tattgcacgt
      661 gaccatctgg ccgatgcgta ctcgcctggt atcgcgcgcc agagccgcgg tgctggtcca
      721 gcattccaaa gtgacttcct gcgtcgggat tggaaccgtg tggaaatggt cgtagagcca
      781 gatagaatga tagctcagcg cctcggcctc ctgcgcgaca cgtgacatgg tttcatacgc
      841 ctcagcaggg tcgggaatac ccaccaggtc catgcgccat ccctgcggaa caagcacgcc
      901 aaaatccatg atgaaccctc ccttcgttat gcatagcccg gaggggcaac gaagccgccg
      961 aactcgcgct caagcagtga ggcgaacgca atcggtgtga aatcctccag gtacgggcca
     1021 atcgcttgca acccgatggg caaaccctgc cgcgtgcgcc cccaggggaa ggccgtcgcg
     1081 ggctgcccgc tcagcgtagc aagccccgga taatcctgta tgcgtccata cgataccact
     1141 tctccgttga tctggagcgt gcgcctggga aacgagaggt cagaactgat gtgtgggaac
     1201 gcaggcgtga tcgtcaccgg cgttaatagc acgtcccagt cgcggaagaa gtcgcggaac
     1261 gcggcgcgat aatgttcgcg cctggcaagg aggccgagga attgggaagc ggtagcggtc
     1321 aagccgaaga gttgcgcctg gacgtaggga tcggaggaat ggcgcagccc ctccacgatg
     1381 cccgcgcgat cctcgtcctt caactcggca aacatgaaga cgtctaagag cgaagtatag
     1441 gtttgctggt acttatgcca atcaaagctt tctggctggg cctccgccac ctttgcgccg
     1501 agcgcaccaa ggcgtgtcgc gaggttttcc agcgccgccg taatttcggc atcgacgggc
     1561 agcgagggaa tctgcggcat gatggcgacg cggaagttcg agagggcgct atggcgcgct
     1621 gcagggagtg tgagatgcca ggccacctct tcgcccacaa cgggtccggc aatcacgtta
     1681 agcgccagcg ccaggtccgc ggcgctgcga gccagtggtc cctgcacacc catgacaaaa
     1741 gcgggattag gcaggggcga gccgggtacg tggccatagc gaggcacggc gctatcgctg
     1801 ggccggtggc catagacgcc gcagaaggcg gcagggatgc ggattgatcc tcctatatcg
     1861 ctgccaaatt ccaggggagt gagacctgcc gccagcgccg ccgcgccccc gccggtactg
     1921 cctcccggtg aacgctctaa atcccacgga ttattggtgc gaccgaagac ctcgttattg
     1981 gcctgccaat cgccggcata gggcggaacg tttgtcttcc cgatcagtac cgcgccagcg
     2041 tctaaaacac gctgcgcaac cggcgcgttc tgcgtggaga tcgcgttggc gcgatgcgtc
     2101 acgcccgccg ttgtgcgcag gccagccact tcgatgcagt ctttgatcgt gagcggcagg
     2161 ccatgcagcg gccccaacaa ctcgccacgc gcgcgggcag cgtcggcctc ggctgcgcgc
     2221 tggcgcgcct gctcgtagtt cgggatcaca atggcattga tgcgcggatt gtagcgttcg
     2281 atacgctgca ggtgttgctc aagcaattcc gttgcagaga cctggcccgt ttgcagggca
     2341 gccagcatag tggatgcaga ggcaaaaggg tcattcataa accaatccag ggacatggtt
     2401 gcgccgacaa taacgtttcc cataaaaagc ttccttctct aaatcccaac atgctgcggc
     2461 tcttacgccc cctcgctgcg cgtccccacg gcactttccc atgcagaagc ccaccggcgc
     2521 gagactcctg ctagttctgg ctcgtgctct cttgccgggg gcgttcaaaa gccgtgtagg
     2581 tcacgacatc ccacgcgttg tggatgccga tgaacaggag cagcagcgtt gccgctgcga
     2641 tgacgaacag ggccggtgcc gcgtgacctg ggagcaggat cgccgcgacg acgaaggcgg
     2701 tgtaggaggc gagcggcaag accgtatgcc acagccagtc ggagaatacc ggctgatagt
     2761 ccgacagacg gtggcgcgcc aaccagagta cgctgaggac gtaggccatc cctccgagac
     2821 ccccaagacc gagcagcaca gcagtattcc agagcgcctg ccagggcgca ttgagaatgg
     2881 cggcaaccag cagcgcggcg caaaaatgca cgacgttggg agtactgaag accgcaatcc
     2941 cgctcctggg cgacgacact tgcacccgta tctgcgcgat caaggcgatg acgacaaaca
     3001 tcaagcccgt caatgtcgcc gcagcggtgc cgacgatgac gtaaaagttt tgccaggtgg
     3061 caagcggcga cagcacagcc tcttgcatga tagatcctcc cttcgatgta catgtcccag
     3121 ctcttcatcc tatcacatcg ctacataatg ctgtcacctg aggacgcact atttcgtttt
     3181 gttaatatgg gaaacttatg caataacaac actagaaatg aaaaagtatg ataaatgcta
     3241 tgcatagaca atcatttcat ctatcgcatt ttcgcctatc taccgtggca ttaagcgggc
     3301 tgttgctctg tctcgtatta ctcagcgcgt gcggctcact gacgacaagt agcgcaagcg
     3361 ttgcgccgac gccaacggtt aatcgcacac tgcaaaacaa gggaaacgcg caactgcaaa
     3421 cgttccagca gtggatcacc ttgatgcagc agtatcaggg cgatgtgacc gcctatcagc
     3481 agcagtacca gggcgaccag caggcgctcc gctcagctca cagcgacgcg gcttaccaga
     3541 aggcgttgaa cacactggat gcgcacgtcg cggctatcca gattccggcg atgaaaaccg
     3601 agagtgggtt cttgtttcag gagctgcaac aggatgccac cagttttggg caacagcata
     3661 cgtatcacga ctcgtataac aacaccacct acccgctcgg ctacgaatat gggccaacgg
     3721 gcgcgaccgg gccgttatgg ttacaagggg agcttgcgtc tgcacaaagc ctggcggact
     3781 accagcaggc cgttgatgat ctgaatatgg acctggcca
//
LOCUS       DASZAK010000181         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_287908, whole genome shotgun sequence.
ACCESSION   DASZAK010000181 DASZAK010000000
VERSION     DASZAK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287908"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VGT44_03070"
     CDS             complement(<1..1216)
                     /locus_tag="VGT44_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579811.1"
                     /translation="MSDTLLEIIARLQQPGAFLLAQGRHILLDQARKRARARLGLLFL
                     LDPAGLLLVEQCGHAPRRLAARDGAPIPVDGLFGAALREQGLLSINENDDDPRILPAE
                     RAWMWHGGRVLLCAVGVGAALDEAIDGTGRQETRRGVMALFTDAHVPAGALSEKAEQD
                     ILLCAALLGSYLDVEEVASDADVTDAPPPDAQLPDAHKGHPYRWADRPASSGVALGGV
                     AAVYEAGLDVDDADRERDEQGVCQRVVDALRDALDATSGGLWLYAPSRGRFVWRAGSG
                     KDSGIERLVGDELAQVAGGLRQGCQFGREQYGARVVTWSPGRMVVLHMLEYGGSSNGS
                     DTVREIVGAVAFVIEGRQDISEEQHLLLAYGCRAAATVVRDWQRRAMERQATIDAERS
                     RIGRDIHDGPAQQ"
     gene            complement(1351..1875)
                     /locus_tag="VGT44_03075"
     CDS             complement(1351..1875)
                     /locus_tag="VGT44_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579812.1"
                     /translation="MATNTAQQTLCPVCHQADQVMKAQKAYERGVTRLAPPTMPVARV
                     GMIKYILIASALVGFGSFFILVFSGTAFGNDSSPANPVQLLQVIITILAIVVALVLSL
                     IALLHVVSGDTKSQKYLPAYDEAVEKWSKLAYCKRDDVVFDPQTNKTISDSALRAMLT
                     IDTTAEPPMIAAAH"
     gene            2279..>2591
                     /locus_tag="VGT44_03080"
     CDS             2279..>2591
                     /locus_tag="VGT44_03080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2579813.1"
                     /translation="MLKELNFVLMHVSDIDTVLPFYTEKLGFEIADQQPGFVQFKQPM
                     GTGAILALSQEGDNTPVQHIELWWLVDDADATHAELAAKGVTIAEPLQDQPFGRTFAI
                     QD"
BASE COUNT          583 a          858 c          637 g          513 t
ORIGIN      
        1 cctgttgtgc cgggccatca tggatatcgc gcccgatacg gctgcgttcc gcgtcgatag
       61 tcgcctgccg ctccattgcg cgtcgctgcc aatcgcgaac aaccgtcgct gccgctcggc
      121 agccatacgc aagcagcaaa tgctgctctt ccgaaatatc ctgcctccct tcgatcacaa
      181 acgcaaccgc gcccactatc tcacgcacag tatcgctgcc gttcgatgag ccgccatact
      241 ccagcatatg caacactacc atgcgcccag gtgaccacgt gacaacccgc gcgccatact
      301 gttcgcgacc gaactggcaa ccttgacgca gcccgccagc cacctgcgcc agctcatccc
      361 caacaagcct ttcaatcccg ctatccttac cagaacccgc gcgccacacg aaccgccccc
      421 gcgacggcgc atacaaccac aagcccccac tcgtggcatc aagcgcatct cttagcgcat
      481 ccaccacgcg ctggcatacc ccctgctcat cccgctctcg atccgcgtca tcaacatcca
      541 gcccagcctc atacaccgcc gccacccccc ccaacgctac accgctggac gcggggcggt
      601 ccgcccacct gtagggatgc cccttgtggg catccgggag ttgggcatcc gggggtggcg
      661 catccgtcac gtcagcatcc gacgccactt cctccacatc aagatagctc cccaacagcg
      721 cggcgcagag cagtatatcc tgttccgcct tctccgatag cgcccctgcc ggtacgtgtg
      781 cgtctgtaaa gagagccatc actccccggc gcgtctcctg cctgcctgtt ccgtctatcg
      841 cctcatccag agcagctccg acgcctaccg cgcagagcag gacacggcca ccatgccaca
      901 tccaggcgcg ctcagcgggc aatatgcgtg gatcatcgtc gttttcattg atggagagca
      961 atccctgttc ccgcaaggcc gcaccaaaaa ggccatcgac cggaataggc gcgccgtcac
     1021 gcgctgccag gcggcgcggc gcgtgaccac attgttctac cagaagcagc ccggctggat
     1081 caagcaagaa taacaacccc aggcgcgccc tggcccgctt gcgcgcctga tcgagcaaga
     1141 tatggcgacc ctgcgccagc aagaatgcgc ccggctgttg caggcgcgcg ataatctcca
     1201 gtagtgtgtc agacattgcc acacgtgtct ccccatgatg caacatgatt ctatagttct
     1261 tgctaccatc atacagggga gaaggaatag ggagagttag cgacgaagga atccatcgct
     1321 aactctccct attcctgagt aggcttttgt ttaatgcgcc gccgctatca tcgggggttc
     1381 ggcggtcgta tcaatcgtga gcatggcgcg cagggctgaa tcggagatgg tcttgttcgt
     1441 ttgcggatca aagaccacgt cgtcgcgttt gcagtacgcg agcttactcc acttctcaac
     1501 cgcttcgtcg taggcgggaa gatatttctg cgatttcgta tcgcccgaca ctacatgcag
     1561 cagggcgatt aaggataaca ccagggccac tactatcgcg agaatagtga tgataacctg
     1621 caacagctgc acaggatttg ctgggctgga atcgttgcca aaagcggtgc cactgaagac
     1681 gaggatgaag aaagaaccga atcccacaag cgcagaggca atcagtatgt actttatcat
     1741 gcccactctg gccaccggca tagtcggcgg tgccaagcgc gtcacaccac gctcatacgc
     1801 cttttgcgcc ttcatcacct ggtctgcctg gtgacacact gggcagagcg tctgctgtgc
     1861 tgtattcgtt gccatatacg aaacaccctc cttgctcgaa gaaacaacga gcgacaattt
     1921 ctttatgaaa aagcatatca cgttctacgc cgttcgacaa tgggcaaatg tgacacgacg
     1981 gctgtaattg cctctactgc ttattatatg aagtttatca cccacctctg gtgctgtcat
     2041 gctgagcgca gcgaagcatc tcgcggctcc tcaaccgaga cccttcgctg cgctcagggt
     2101 gacaatctct tcccgaactg ggttagtaaa aatcatataa tcgctcgaat cctgttccgg
     2161 caaaattttc gttccgctgc tgacatactg ctgtcactcg ctcctggcat acttacagtg
     2221 tgagcagcag ctcaaacaaa accagcgacg ataacacatg aaaggaacac aacgctccat
     2281 gttaaaggaa ctcaacttcg tcttgatgca cgtcagcgat atcgacacag tcctgccctt
     2341 ctacaccgaa aagctcggtt tcgagatcgc cgaccagcag cccggcttcg tacagttcaa
     2401 gcagccaatg ggaaccggcg ctatcctcgc cctttcacaa gagggtgata acacgcccgt
     2461 gcagcacatc gaactctggt ggctggtaga cgatgccgac gccacccacg ctgaactggc
     2521 cgcgaagggc gtgaccatcg cggaaccatt gcaggaccag ccattcgggc gcaccttcgc
     2581 cattcaagac c
//
LOCUS       DASZAK010000182         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_608527, whole genome shotgun sequence.
ACCESSION   DASZAK010000182 DASZAK010000000
VERSION     DASZAK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608527"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..>1577)
                     /locus_tag="VGT44_03085"
     CDS             complement(144..>1577)
                     /locus_tag="VGT44_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2579814.1"
                     /translation="KNISVDQFIRLLIQFSPNSKTAPLGLTLLGMIAAGTGLGLLYAL
                     AARVRLPAVGLRPARSEWLIAAGFALAMTLAGAILFWGEIGQSFTGLPLAWATLLTLL
                     GLLVDFACYAVVLCLSYRALLPKQPAANSAGDAAQQTKNARRQLLARAGVAVLGVGAA
                     GGSVGLIKGLFTNYASYDGMQTFTQNGFIPPITPNSDHYVVTQNSIDPAVNAGLWRLE
                     ITGLVGSPGTYTLDELQALPSTTRAVTLECIANYIGGHLISTAIWQGITLRDLLARHG
                     GALPNATHMAFYGVDGYAVSQPLDVVMQADALLAWRMNGADVPIRHGYPLRALIPGRY
                     GEENAKWVTRIELTDHFVGGLYSDQGWYNGPLHTITRIDRPFGHVELAFTIEIGGIAF
                     AGNRGIQQVEVSVDGGQTWNIAQLQPALSPDAWVFWTWQWQPLLAGKYTIVARSTDGT
                     GALQTEQKQGTVPNGATGYHVIEVRVG"
BASE COUNT          311 a          479 c          495 g          292 t
ORIGIN      
        1 catgaagctg gcgtgttcat cccactgctc ctgctcgcgc atcgctcgcg aggcatccca
       61 gttctctcct cgttctcgcg tcactgcata atagatgaca ggatttttgg acatcggtat
      121 ctccttgtga gaaagcagac actttatcct acccgcactt cgatcacatg ataccccgtc
      181 gcgccattcg ggaccgtccc ctgcttctgc tcggtctgga gcgcgccagt gccgtcggtg
      241 gagcgcgcca cgatggtgta tttgccggcg agcagcggct gccattgcca cgtccagaag
      301 acccaggcat ccggcgaaag ggcaggttgc aactgggcga tgttccaggt ctgaccgcca
      361 tcgacactga cctcgacctg ctggatgccg cgattgccgg cgaaggcgat accgccgatt
      421 tcgatggtga aggcaagctc aacgtggccg aatggccgat cgatgcgggt tatagtgtgc
      481 aatgggccat tataccagcc ctggtcagaa tagagaccac cgacgaagtg atcggtcagt
      541 tcgatgcgcg tcacccactt ggcattctcc tcgccgtagc gaccggggat gagggcgcgc
      601 agggggtagc cgtgccggat tggcacatcc gcgccgttca tgcgccaggc aagcaaagca
      661 tcggcctgca tgaccacgtc gaggggctgg ctgacggcgt agccatcgac gccgtagaag
      721 gccatgtggg tcgcgttcgg cagagcgccg ccgtgtcgtg ccagcaggtc gcgcaacgtg
      781 atgccctgcc agatggcggt gctgatgaga tggccgccga tgtagttcgc gatacattcc
      841 agcgtcacag cgcgtgtcgt cgaaggcagg gcttgcagct catcaagcgt atacgtgccg
      901 ggactgccga ccagtccggt aatttccagc cgccagaggc cagcgttgac ggcaggatcg
      961 atactattct gcgtgacgac atagtgatcg ctattgggcg taattggcgg aataaagccg
     1021 ttctgtgtga aggtctgcat cccatcgtag ctggcatagt tggtgaacag accctttatc
     1081 aggccgaccg aaccgcctgc cgcgccgaca cccagcacag caacacccgc gcgcgcaagt
     1141 aactgccgcc gggcattttt ggtctgctgc gcagcgtccc ctgcgctatt cgccgcgggc
     1201 tgtttcggca ggagggcgcg ataggacagg cagagcacga ccgcgtagca ggcgaagtca
     1261 accagcaggc cgagcagcgt gagcagcgtg gcccacgcga ggggcaggcc ggtgaagctt
     1321 tgaccgatct ccccccagaa gagtatcgcg cctgccagcg tcatcgccag agcaaacccg
     1381 gctgctatca gccattcact gcgagccggg cgcaggccga ctgccggcag cctgacacgc
     1441 gcggcgaggg cgtagagcag gcccagcccg gtgccagccg cgatcatgcc gagcagcgtc
     1501 aagccaagcg gagccgtttt ggaattaggg ctgaattgaa tcagcagccg gataaattga
     1561 tcgaccgaaa tgttttt
//
LOCUS       DASZAK010000183         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_76196, whole genome shotgun sequence.
ACCESSION   DASZAK010000183 DASZAK010000000
VERSION     DASZAK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76196"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VGT44_03090"
     CDS             complement(<1..722)
                     /locus_tag="VGT44_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2579815.1"
                     /translation="MYVIPEAFKREMIGLHGEDGGGAWLERLPAILASCEERWNLKVS
                     APFLDVSFHYVVPARRADGTGVVVKACSPTDEFHHEKEALQCFDGHGMARLLDYDDVQ
                     EVLLLEYLQPGKQLSTLEDDEEATSHAAAVMSELWRPAPQHHSFQTVQDWGEGFNRLR
                     QHYNGGNGPFRPALLEEAETLFAELSATMSEPVLLHGDLHHFNILSAQRSPWLAIDPK
                     GLIGERAYETGSLLRNPYPELL"
     gene            complement(1090..1569)
                     /locus_tag="VGT44_03095"
     CDS             complement(1090..1569)
                     /locus_tag="VGT44_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl tetrahydropterin synthase family
                     protein"
                     /protein_id="HEV2579816.1"
                     /translation="MRKVRVEGGNLKFNAAHFITFGGGAERLHGHNYTVQIEMEGTLN
                     QDNLVFDFSVLKRLTREVCERLNHHFILPLRNPNLVWNELPDEWEILFRQKRYLLPRE
                     DVIALPIDNSTAECLAEYICGEVCAALADYDIANIHALLVGVAEAPTQVAYYQVKLS"
     gene            complement(1603..>2115)
                     /locus_tag="VGT44_03100"
     CDS             complement(1603..>2115)
                     /locus_tag="VGT44_03100"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2579817.1"
                     /translation="ATLARLERAQLRYLSSGAQNGKQPQFSGGIGRVARDITGVPQSY
                     QQAQQALEIGSRLFGDGKLHSFAQLGIYRLLFHLYEHRELVQFYEETLGPLVDADTRN
                     NHALIDTLENFFRYNGNLSETARAMHFHRNSLLYRLGRIEELLGRSLEDPELRLSLQM
                     ALKIRHLLER"
BASE COUNT          448 a          562 c          604 g          501 t
ORIGIN      
        1 tcgagcagtt cgggataggg gttgcgcagc agcgacccgg tttcgtaggc tcgctctcct
       61 atcagtcctt tggggtcgat ggccagccag gggctacgct gcgcggagag gatgttgaag
      121 tgatgcaggt cgccatgcaa cagcaccggc tcagacatcg tggcgctgag ttcggcaaag
      181 agcgtctcgg cttcctcgag caacgcgggt cggaacgggc cattgccgcc gttgtaatgc
      241 tggcgcaatc tgttgaaccc ttcaccccaa tcttgcaccg tctggaatga atgatgctgc
      301 ggagctggtc gccacaattc gctcatgacg gcggctgcgt gtgaagtggc ctcctcgtca
      361 tcttccagcg tgctcaattg cttgccaggt tgcaggtatt ccaacaataa tacctcctgg
      421 acatcgtcgt agtcgagcag ccgcgccatg ccatgtccgt caaaacattg cagcgcctct
      481 ttctcatgat gaaattcatc tgtaggcgaa catgctttga ctacgacgcc tgtgccatcg
      541 gcgcgccttg ccgggacaac atagtggaaa gatacatcga gaaacggcgc gctgactttg
      601 aggttccagc gctcttcgca gctcgccaga attgcgggca ggcgctccag ccacgctcct
      661 cccccatctt cgccatgcag cccaatcatt tcgcgtttga atgcttcggg aatcacgtac
      721 atctatgctt tgctccattc ttgggttcat ttgtgtgcaa taatgggcga tcaaaccgcc
      781 atattgttct gacaagattc aaatgacctg taggtgccga ttgatcggcc tggttggttt
      841 gccgatgacg cccatggagg ccgatcaatc ggcggtgtgc gcgataaatc ggcacctacg
      901 gttgggtggc gactcaatca tgtggagggg cgcgcgggca tatggatact atgaggaagc
      961 agcagaaagt ggattcatgg tcacgaagat gaatcaggta tagaaagcgg tattcacttg
     1021 cggtgaacga acgtcaataa cgggtgaact tcatgactat tccttagcga gggtcggtcc
     1081 actaaatcgt tacgatagct tcacttgata gtatgccacc tgcgttggcg cttccgcaac
     1141 ccctaccagc agcgcatgaa tatttgcaat atcgtagtct gccagggccg cgcatacttc
     1201 gccgcagatg tattcggcaa ggcattcagc ggttgagtta tcgatgggca gggcaatcac
     1261 atcttcgcgg ggcaggaggt agcgtttctg acgaaaaagt atctcccatt cgtccgggag
     1321 ttcgttccat accaggttgg gattgcgtag agggaggatg aagtgatgat tgaggcgctc
     1381 gcacacttcc ctggtaaggc gcttgaggac ggagaaatcg aagaccaggt tatcctggtt
     1441 gagtgtgcct tccatttcga tctgcactgt atagttatgc ccatgcagcc gctcagcgcc
     1501 tccgccgaac gtgataaaat gggcggcgtt aaacttgaga ttgccgcctt ctactctcac
     1561 tttacgcatg ggctgtccat tcatttcgtc cataacttgt cttcaccgtt ccaggagatg
     1621 gcgaatcttg agcgccatct gcaacgacag gcgcagttcc gggtcttcca gcgagcgacc
     1681 cagcagttcc tcgatgcgtc ccaggcgata gagcagcgag tttcgatgaa aatgcatagc
     1741 gcgcgctgtt tcgcttagat tcccattata acggaaaaag ttctccaatg tatcgatcag
     1801 tgcgtggttg tttctggtat cggcatccac aagcgggcca agcgtctctt cataaaactg
     1861 cacgagttcg cggtgttcat agagatgaaa gagcaagcgg tagatgccaa gctgagcgaa
     1921 ggaatgcagt tttccgtccc cgaacaggcg gctgccgatt tccagagcct gctgggcctg
     1981 ctggtaggat tgtggcacgc cggtgatatc cctggccacg cgcccgatgc cgcctgagaa
     2041 ttgcggctgc ttgccatttt gcgcgccgct cgacaggtag cgtaactgcg cgcgttccag
     2101 ccgggcgagt gtggc
//
LOCUS       DASZAK010000184         5334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_4930, whole genome shotgun sequence.
ACCESSION   DASZAK010000184 DASZAK010000000
VERSION     DASZAK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4930"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..930
                     /gene="larB"
                     /locus_tag="VGT44_03105"
     CDS             187..930
                     /gene="larB"
                     /locus_tag="VGT44_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEV2579818.1"
                     /translation="MSQFADESWDTMLSVFGQDGVIEVRPDHQRERRKGAPEVIFGET
                     KEAAQIVVMARALLANSGRAIISRIKPEAISGVKDAFAAYRVQVYEAAHTMTIYAPEY
                     TPRSTGGKVAVISAGTSDIPVAEEAGLIAGEMGCQVTSIYDVGVAGLHRLFGPLNELL
                     TQGVDVIIVAAGMDGALPSVVAGLVPVPVIGLPTSIGYGMGGKGVAALLAMLQTCAPG
                     LSVVNIDNGVGAGITAGLIANRVAEARSR"
     gene            1078..1350
                     /locus_tag="VGT44_03110"
     CDS             1078..1350
                     /locus_tag="VGT44_03110"
                     /inference="COORDINATES: protein motif:HMM:NF022150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEV2579819.1"
                     /translation="MANNKRGPEPGTQQARRGGQAVRAKYGPEFYAKIGKKGGDTVKE
                     KRGPQFYAEIGKKGGESTKRQQGSEFYSKIGKKGGEHGRVSASEKE"
     gene            complement(1382..2989)
                     /gene="ftsZ"
                     /locus_tag="VGT44_03115"
     CDS             complement(1382..2989)
                     /gene="ftsZ"
                     /locus_tag="VGT44_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV2579820.1"
                     /translation="MQPKPHEDANQRNNPPQPGKGMPRREAKGREPYPDQGDQWAVLR
                     HEMEGEEKREMLARGSRQSDLDQRDTEEDAVTSQTPSDYYDDAPNQVDIVVAGIGGAG
                     MNAVNRMISTRVRGVRFVAMNTDAQVLDISQAGARICLGQRYTKGLGSGGNANVGARA
                     AAESAEEIQAALGNADLVFIAAGMGGGTGTGGAPVVASLAKKLGALTIGIVTLPFTFE
                     GYRRKKVAGEGLASLGSEVDALITIPNDRLLDVTGRDRSLADAFKIADDVLRQGVQGI
                     AEVINVPGLVNVDFADVRSVLREAGSALMSIGQGQGRNRAQQAAEAAIAGGFLNVAIR
                     GAQRVLFNISGGEDMTLFEVNEVAELIGNAVDTAADITFGAVIDPTLDDKIRVTLIAA
                     GMQEMRTARIPAVHQEQPLPSRASNASFAPGRIPPIQPSPPATNIPTSPRQPNNYAGE
                     RYGDSHFPAISHTPVPPDVEPMRPATSPARPPAPQGRPQRSLDDLRGLRSMGRRQEML
                     RSQRDARESGDYEDEGIDMPPFMKRYQ"
     gene            complement(3317..3904)
                     /locus_tag="VGT44_03120"
     CDS             complement(3317..3904)
                     /locus_tag="VGT44_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV2579821.1"
                     /translation="MSRICVFTGSNYGNRPEYRRLAQALGEELARRGLGLVYGAASIG
                     LMGVVADAVLARGGEAIGVIPRALFKREVTHRGLTQLHEVGSMHERKALMADLADGFI
                     ALPDGLGTFDELFEIITWAQLGLHTKPIGLLDDTGYFTPLLALIHHASAEGFIMPAHA
                     NIVMCEATPAALLDRFAAYTPIVNPNKWVEPPPER"
     gene            4085..>5334
                     /locus_tag="VGT44_03125"
     CDS             4085..>5334
                     /locus_tag="VGT44_03125"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2579822.1"
                     /translation="MNPEQLIGRTLGHYHILRQVGYGGMATVFLAEDTNLEREVAVKV
                     FWPRPGETRDFLRRFAREARVLAQLDHPNILSVYDYGETDGIAYLVTPYMPGGTLKDL
                     LKERKTLSPVESLRLVAQMLNALQYAHERGLIHRDIKPGNMLFKADGSLVLADFGLVK
                     VATPELAPGAQLDLTNQTGTAITGTPEYMSPEQIEGKAFPASDIYSVGIVIYEMLTGK
                     RPFQSDSAMGLLMQQMHQQPHSLRDSNPYISPQLDAAVLRALEKDPARRYSRPADFLQ
                     ALVQASSPGAQNNADVRRLNELTVPVDWVTPGQRPSQDAPIKPVGAGSSPAFPPQAVQ
                     AQHGPITHPGQTQPAGPSLPITPMRQFPQYVAPQQAPYAPQQTKRRRGGLLVTIFSLL
                     VIGVIVTGLALNVTGHLPFVNLGQT"
BASE COUNT         1057 a         1555 c         1648 g         1074 t
ORIGIN      
        1 gtgtagatta tagcatagac aatcaaccaa ccgtaggcgc cgatttactg tgcgcctacg
       61 atttgtacgc tgctcagcaa tacacagttg gcttgctcat ttctcgtgtg tatgatacac
      121 tgagtctggt tggaaaactg ccaaacgaga tgaatctaca aggctctagt aaggatgtag
      181 cgtactgtga gtcaattcgc cgacgagtcg tgggatacca tgctgtctgt cttcggccag
      241 gacggggtga tcgaggttcg gccagatcac cagcgcgaac ggcgcaaggg agcgcctgag
      301 gtgatttttg gcgaaacgaa agaagcagcg cagattgtgg tgatggcgcg cgcgctgctg
      361 gcaaattcgg gcagggcgat catcagcagg atcaagccgg aggcgatcag tggggtgaaa
      421 gatgccttcg cggcctatcg cgtgcaggtg tacgaggcgg cgcacaccat gacgatctac
      481 gcgccggagt atacgccgcg cagcactggg ggcaaggtcg cggtcatcag cgcgggaacg
      541 tccgacattc ctgttgcgga ggaggcaggg ctgatcgcgg gcgagatggg ctgccaggtg
      601 acaagtattt atgatgtcgg cgtggcgggt ctgcaccggc tcttcggccc attgaacgag
      661 ttgcttactc aaggagtgga tgtcattatc gtcgcggcag gcatggatgg cgcgctgcca
      721 tcggtcgtgg ccgggttggt acccgtaccg gtgattggtc tgccaacctc gatcggctac
      781 ggcatgggtg gcaagggggt cgccgcgctg ctggcgatgt tacagacatg cgctcccggc
      841 ctctcggtgg tcaacattga caacggcgtg ggagcaggaa tcacggcggg gttaatcgcc
      901 aaccgcgtcg cggaggcgcg ctcacgatga ttcgcgcggt gcgtaggatg catcggttgt
      961 tgcaggaaca tgagtcgcag tagggaagca gcgagatgtt ctgtctgtcg gaatgtctca
     1021 ccgtttcgca agttgggtac acgtggtaac gagtttacaa gaagggcggg actttctatg
     1081 gcgaacaaca aacgaggacc ggaaccggga acacagcaag ccaggcgcgg cggccaggcg
     1141 gtacgcgcca agtacggacc agaattttac gctaagatcg gcaagaaagg cggcgatacc
     1201 gtgaaggaga aacgcgggcc gcagttctat gccgaaatcg gcaaaaaggg cggcgaatcg
     1261 accaagcgcc agcagggatc tgagttctac tccaagattg gcaaaaaggg tggcgaacac
     1321 ggtcgcgtga gcgcctccga gaaggagtga tgccagggcg accgcaaggg tcgccctggc
     1381 atcactgata tcgcttcatg aagggtggca tgtcgatgcc ttcatcttcg tagtcgccgc
     1441 tctcgcgggc atcgcgctgg ctgcgcagca tctcctggcg gcggcccatg ctgcgcaggc
     1501 cgcgtagatc gtccaggctg cgctgcgggc ggccctgcgg cgcgggcggg cgagccggtg
     1561 aagtcgcggg ccgcatcggc tctacgtcgg gcggcacggg cgtgtgtgag atggcaggga
     1621 aatgtgagtc tccataacgc tcgccagcat agttgttcgg ctgccgggga gaggtgggta
     1681 tattggtggc gggtggtgac ggctgaatgg gcggtatgcg tcccggcgcg aacgaagcat
     1741 ttgacgccct cgatggcagc ggctgctcct gatgcacggc aggaatacga gcggtgcgca
     1801 tctcctgcat cccggcggcg atcaacgtca cacgaatttt atcgtccagc gtgggatcga
     1861 tgaccgcgcc gaaggtgatg tcggcggcgg tatcgacggc attgccgatc aattccgcga
     1921 cctcattgac ctcgaacagc gtcatatcct cgccgccgct gatgttgaag agcacgcgct
     1981 gcgcgccgcg aatcgctacg ttgagaaagc ctcctgcaat cgctgcttct gccgcctgct
     2041 gcgcgcgatt gcggccctga ccctggccaa tgctcatcag ggcgctcccg gcctcgcgca
     2101 gcacactgcg cacgtcggcg aaatcgacat tgaccagccc cggaacgttg atgacctccg
     2161 cgatgccctg cacaccctgg cgcagcacat catcagctat cttgaaggcg tcggccaggc
     2221 tgcgatcgcg cccggtgacg tccagcaggc gatcattggg aatggtgatg agcgcatcca
     2281 cctcggagcc gagcgaggca agcccttctc cggcgacctt cttgcgtcga tagccctcga
     2341 aggtgaaagg cagcgtgacg atgccgatgg tgagcgcgcc aagcttcttt gcgagcgagg
     2401 ccacgacggg cgcgccccct gtgccggtgc cgcctcccat gcctgccgcg ataaaaacga
     2461 ggtcggcatt gcccagcgcg gcctgaattt cttccgcgct ttcggcggcg gcgcgcgcgc
     2521 ccacgttggc attgccgcca gaaccaagtc ctttggtata gcgctgaccc aggcagatgc
     2581 gcgcccccgc ctgggagata tcgagtacct gcgcgtcggt gttcatggcg acgaagcgca
     2641 cgccgcgcac gcgcgttgaa atcatgcggt tgaccgcgtt catgcccgcg ccgccgatgc
     2701 ccgccacaac gatatcgacc tggttgggag cgtcgtcgta gtaatcagag ggagtttgtg
     2761 aggtaacagc atcttcctcc gtatcgcgtt gatccaggtc agactgcctc gaccctcgtg
     2821 ccagcatctc tctcttctcc tccccctcca tctcatgtcg aagcaccgcc cactgatcgc
     2881 cctggtcagg atacggctcg cgtccttttg cttcacggcg cggcataccc tttccaggtt
     2941 gcgggggatt gttgcgttgg ttcgcgtcct cgtgtggttt tggctgcata aaccccctcc
     3001 tcttgctttc cggcgcatct ggcccggtgt accgttgtta caagcaagta tagcacacgc
     3061 cgaaatagtg ttctatccgc attgtaacaa cggtgtcaag gtcaggatgc cgccttgctc
     3121 tgcttggcag ccttcttctc tttcaacatc tccttcttct gcttgttgga cttcggggga
     3181 ctcttctgcg ttttcttttg ctctttattg gccataaatg tgacatcctc cttctgaatt
     3241 ccaaaaatgc gccagcgtct ccaggacgcc gctaccacta ttgtaccaaa ttcgatcgat
     3301 aagggatagc tccctattac cgttcgggcg gtggttcaac ccatttattc ggattgacga
     3361 tgggcgtata cgcggcgaag cggtcgagca gcgcggcggg agtggcttcg cacataacaa
     3421 tgttggcgtg agccggcatg ataaagccct ccgcgctggc gtggtgaatg agagcgagca
     3481 gcggtgtgaa gtagccggtg tcgtcgagca ggccgatggg tttggtatgc aatcccaact
     3541 gcgcccaggt gatgatctca aaaagttcat cgaatgtccc caggccgtca gggagggcga
     3601 taaagccatc ggccaggtcg gccatcagcg ccttgcgttc gtgcatactg ccaacctcgt
     3661 gcaactgcgt caggccgcga tgcgtcacct cgcgcttgaa gagggcgcgc ggtatcacgc
     3721 cgatggcctc gccgcctcgc gccagcaccg cgtcggcaac cacgcccatc aatccaatac
     3781 tggccgcgcc gtagaccagc cccagcccgc gcctggccag ttcctcgccc agcgcctggg
     3841 ccaggcggcg atactcaggc cggttgccat agtttgagcc ggtgaagacg cagatgcggg
     3901 acatggatgt gttcctcctt atgatgagat gataactcat gaaattatat tatatacatt
     3961 aaccggcttc taccgtttgt ttggtcgcca taactatttt ctctaataaa cccataccat
     4021 aacgtataat aggcatactg acaacttgcg cacgacataa aaagcatatg gagtaaatcg
     4081 tcatatgaac ccggagcaat tgatcggacg gaccctgggg cattatcaca tcctgcggca
     4141 ggtggggtat ggtggcatgg cgacggtatt tctggccgag gataccaacc tggagcgcga
     4201 ggttgcggtc aaggtgttct ggccgaggcc gggggagacg cgcgatttcc tgcggcggtt
     4261 cgcgcgcgag gctcgcgtgc tggcgcagct cgatcacccc aatatcctgt ccgtctatga
     4321 ctacggcgaa acggacggca tcgcttacct ggtcacgcct tatatgcccg gcggcacgct
     4381 caaggatttg ctcaaagaac gcaagacgct atcgccggtc gagtcgctgc ggctggtggc
     4441 tcagatgctg aatgccctgc aatacgccca cgagcgcggg ctgatccatc gcgatatcaa
     4501 gcccggcaat atgctgttta aggccgacgg cagcctggtg ctggcggatt tcgggctggt
     4561 caaggtggct acacccgaac tggcgcctgg cgcgcagctt gatttgacca accagaccgg
     4621 cacggctatc accggaacgc cggaatatat gtcgccggag cagatcgagg gcaaagcgtt
     4681 tccggccagc gatatctact cggtcggcat cgtgatctac gaaatgttga cgggcaaacg
     4741 tcctttccaa tcagattcgg ccatgggact cttgatgcag cagatgcacc agcagccgca
     4801 ctcgctgcgc gacagcaacc cctacatctc accgcaactg gatgcagcgg tgctgcgcgc
     4861 gcttgagaag gaccctgccc ggcgctactc ccgccccgca gacttcctgc aggcgcttgt
     4921 gcaggcctcc tcgcccggcg cgcagaacaa tgctgatgtg agacgtttga atgagttgac
     4981 ggttcccgta gactgggtga cgccgggaca gcgaccatcc caggatgccc caattaagcc
     5041 ggtcggcgca ggaagcagtc ccgcattccc accgcaggct gtccaggcac aacatgggcc
     5101 gattacgcat ccggggcaga cgcaaccggc aggtccatca ttgccgatca cgccgatgcg
     5161 ccaattcccg caatacgtcg cgccgcagca ggccccttac gcacctcagc agacgaaacg
     5221 gcgcagagga ggtttactgg ttaccatatt ctctctactg gtgatcggcg taattgtaac
     5281 agggctggca ttgaacgtca ccggccactt gccatttgtg aacctcgggc aaac
//
LOCUS       DASZAK010000185         2878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_501940, whole genome shotgun sequence.
ACCESSION   DASZAK010000185 DASZAK010000000
VERSION     DASZAK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501940"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="VGT44_03130"
     CDS             <1..842
                     /locus_tag="VGT44_03130"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEV2579823.1"
                     /translation="KMAALCTLLHATQGESSLIFGRTKHGVEKLGKQLSKLGFTVGTL
                     HGNKSQRAREEVLTAFRRGHVQTLLATNVAARGLDITGIDQVINYELPESSDLFTHRI
                     GRTGRMGRQGKAITLLVPSDVPKWRRMAHNLGQGVALQQLVINEEALVTKPLASSEAS
                     SMQEHVQNGEQRLVRELKHFSPGQERGRQAQAERERDATSGTKRRRKERVTGGAPART
                     SARQPEGFPFAERTYSGKPRDDRRGAGKQSGFRKARGKHNPFDFAATAPRHRTVRRKL
                     RAI"
     gene            complement(852..2078)
                     /locus_tag="VGT44_03135"
     CDS             complement(852..2078)
                     /locus_tag="VGT44_03135"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV2579824.1"
                     /translation="MVLKQMSNGGLSRARLGRMHHVMIGYVERGEVPGLVTLVSRRGE
                     VHIDAIGMMAVGSNDPMVRDTIFRISSMTKPITAVATMILVEECKLRLDEPVDRLLPE
                     LAERKVLKRLDGPLDDTVPANRPITVRDLLTFRMGFGQMMAFPDAYPILKAANEQQIG
                     MGPPSPSAMPAPDEWIRRLGSLPLMHQPGEQWTYNTGSDVLGVLIARASGQPFETFLR
                     ERIFEPLGMKDTSFSVSEASLDRLATSYWTNFETGALEIYDEARGGQWSHPPAFPSGA
                     GGLVSTIDDYLAFGQMMLNQGKHGSERILSRLSVETMTTDQLTPEQKALSGLVSGSFD
                     SNGWGFGVSVITRRDDVAAVPGRFGWDGGLGTSWYSDPREGMVTILMTQRAWTSPSPP
                     NVCLDFWTSAYQAIDD"
     gene            2443..>2878
                     /locus_tag="VGT44_03140"
     CDS             2443..>2878
                     /locus_tag="VGT44_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEV2579825.1"
                     /translation="MMDIVFASTGQIAAAIQAGHVSAMEVLEAHLAQIATHNPTLNAI
                     ITMDAARSREQAREADEALARGELWGPLHGVPFTLKDAHATSGMRTTTGFPPLASYVP
                     QEDSTVTARLKAAGGILIGKTNVPVMLADYQTNNPIFGRTNNP"
BASE COUNT          662 a          821 c          821 g          574 t
ORIGIN      
        1 agaaaatggc ggcgctctgt acgcttttgc acgccacgca gggtgaaagt agcctgatct
       61 ttggccggac gaagcacggt gtcgagaagt tagggaaaca actcagcaag ctcgggttta
      121 cagtgggaac attacacggt aataagagcc agcgcgcacg tgaagaggtg ctgaccgcgt
      181 tccgccgggg gcatgtgcag acactgcttg ccaccaatgt agcagctcgc gggctggaca
      241 ttacagggat agatcaggtt atcaattacg agctgccgga atcgagcgat ctttttacgc
      301 accgcattgg ccgcacgggc agaatggggc gacaaggcaa ggcgattacg ctgctggtac
      361 catcagatgt ccccaaatgg cggcgtatgg cgcacaattt agggcagggc gtcgcattgc
      421 agcagctggt tattaatgag gaggcccttg ttacgaaacc acttgcaagt tcagaggcct
      481 cgagtatgca ggaacacgtg cagaacggcg agcagcgact ggtacgtgaa ctcaaacatt
      541 tctcgccggg tcaggagcga ggcagacaag cacaagcaga gcgtgaacgt gatgccacat
      601 caggcacgaa aaggcgaagg aaagagcgtg ttaccggtgg agcaccagcg cgcacatctg
      661 cacggcagcc tgaaggcttt ccctttgcag aacgcaccta ttccggcaag ccgagggatg
      721 acagaagagg tgcaggaaag caatctggct tcaggaaagc cagagggaag cacaacccat
      781 tcgattttgc tgcgactgcg ccgcgacacc ggacagtcag gagaaagctc agagcgatat
      841 aggctggcac ctcagtcgtc gatcgcctgg taggcagatg tccagaaatc aagacagaca
      901 ttgggagggc tgggggaggt ccaggcacgc tgggtcatga ggatggtgac catgccctcc
      961 ctgggatccg aataccagga agtgcccaat cctccatccc agccgaacct tcccggtact
     1021 gccgccacgt cgtcgcgcct ggtgatcacg gagacaccga acccccagcc gttactgtca
     1081 aaggagccag agaccaggcc ggacagcgct ttctgctcgg gcgtcaactg gtcggtcgtc
     1141 atggtctcga ccgaaagcct tgagaggatg cgctcgctgc cgtgtttgcc ctgattcagc
     1201 atcatctggc cgaaagccag atagtcgtcg atggtcgata ccagacctcc tgctccggac
     1261 gggaatgcag gcggatggct ccattggccg cccctggctt cgtcatagat ctcgagcgcc
     1321 ccggtttcga aattggtcca gtagctggtc gccaaccgat cgagcgaagc ttctgacacg
     1381 ctgaaactgg tgtccttcat gcccaacggc tcgaagatgc gttcgcgcaa gaacgtctca
     1441 aacggctgac ccgaggcgcg cgcaatcagc acgcccaaca cgtcggaccc tgtgttgtac
     1501 gtccactgct cacccggctg atgcatgagc ggaaggctcc ccaggcggcg tatccactca
     1561 tcgggcgcag gcattgctga cgggctcggt ggccccatgc caatttgttg ctcgttcgct
     1621 gccttcagga tcggatacgc gtccggaaag gccatcatct gcccgaagcc catgcggaaa
     1681 gtgagcagat cacgcacggt gatcggtcgg ttcgcgggca ccgtgtcgtc gagcggccca
     1741 tccagtcgct tcaaaacttt gcgctcggcc agttcgggaa gcaaacggtc gaccggctcg
     1801 tctagccgca gcttacattc ctccacgagg atcattgtgg ccacggccgt gatcggcttg
     1861 gtcatcgagc tgatgcggaa gatcgtgtcg cgcaccattg ggtcgttgct gccgactgcc
     1921 atcatgccta tcgcatcgat atgcacctcg ccccgtcggc tgaccagcgt gacgagaccg
     1981 ggcacttcgc cgcgctccac atagccgatc atgacgtgat gcatgcggcc gagccgcgcc
     2041 cttgacaatc cgccgttgct catttgctta agcaccattt ttatcctcct gtgtgtcgag
     2101 ctctaaaagc attttcccat caatgctact ctaacatcac tatttcgcca caagttcggc
     2161 gaagcgatcc atggactcga acgcctgaga gatggccggt gaaacctatc tcatcaatga
     2221 accgatacaa tcatgtgttc tcactgcttt aagtatacat gaatgcaaca gcaaaaactt
     2281 cttctaactc accattttcg aacggatgtc cgctcttcaa taagagtaag ctagaagaag
     2341 tttttgcttt tcagtatgct atgatgatca agcgaccaga agttgaccaa ctcattcgct
     2401 gtcattgctg aaatgagacg cagaacgaga tatggaggtt ttatgatgga tattgttttc
     2461 gccagtacgg gacagatagc cgctgccatc caggcaggcc acgtttcagc tatggaagtg
     2521 ctggaagcgc acctggcgca gattgctact cacaatccta ctctgaacgc tatcatcacg
     2581 atggatgccg cgcgttcgcg tgagcaggcg cgggaagcgg atgaagcact agctcgcgga
     2641 gagctgtggg ggccgctgca tggcgtgccc tttacgctga aagatgccca tgcgacgtcg
     2701 ggcatgcgca ccaccaccgg gttcccgccg ctggccagct acgtgcccca ggaagatagc
     2761 accgtgaccg cacgtctcaa ggcggcgggc ggcattctca tcggaaaaac gaacgtacct
     2821 gtgatgctgg ccgattatca aaccaataat cctatctttg gccgcaccaa caatccct
//
LOCUS       DASZAK010000186         5211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_40019, whole genome shotgun sequence.
ACCESSION   DASZAK010000186 DASZAK010000000
VERSION     DASZAK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40019"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VGT44_03145"
     CDS             <1..840
                     /locus_tag="VGT44_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2579826.1"
                     /translation="MGIPAKRQWSWLPSFAVCCVVATVVLIPLLATLLGGLRTNGELL
                     TEPFSIPKTLHWENFGLVLASDSPFWGEIVNSLVTVTGTVVLLLFAACPAAFVLARIS
                     FRGREVVFNFFLFGLLFPLTVAVLPLYITIRQFGLLDSLWGVILPQAAFGLPITILIL
                     RNFFRAIPIELEDAATIDGASKPIFFWRVLLPLARPAIAVVSILAVVSSWNNFLLPLL
                     VLTDQSQWTLPIGATQFQQQYATDWPSILAFVTLAMIPAILIYLLAERQIVAGLTAGA
                     VKG"
     gene            846..2864
                     /locus_tag="VGT44_03150"
     CDS             846..2864
                     /locus_tag="VGT44_03150"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV2579827.1"
                     /translation="MTVTLTEQGLEIDEQRVPVYSGSVHYWRLERDKWPVILDQVQSL
                     GFGMVETYIPWSVHEIAPGTFDWGSVDPRKDIEAFCQLCEARGLYLMVRPGPLINAEM
                     TDFGFPEWVLLDPEVQAHTAVGSIHLDAAWGFHPPRPFPVPSYASPKFYEAVGRWFDA
                     VCPVIERHMAPGGCIVAVQSDNESCYLFHDQPYATDYSPASIAAYQRFLCERYETIVQ
                     LNAVYGQDFRGFDAIAPPRDCQIRTAADVPWHRDWVEYKEHYLHQGVVQIAQMLRARG
                     IKGIPIFHDVAFQYSTPLDIARMEADPAIDWVGMNLYRSPQKYVGTIQRIRYLAGTTR
                     LPFVPEFGSGIWSHHSQTPTPVEQEFTTLAALMHGMRAVNFYMLVERERWQGSPITRH
                     GAYRPGYAEFYQRLSIFLQRYPLGTFHRQTQVVVLLNYDLGRYKAMASTLHYAHIDLL
                     RLPEELEVVDLDLGFRWDVAHEADQHRSDNWLGKVCAQLKQQQLDYDIADSHIDPRRL
                     QQYAITCIPTVDFMDAADQQQLLGYVAAGGCLIIGPGVPYLDSNLRPCSVLNAVVQEP
                     GEVAFGCGHIIWVSADALATTLAQRLPSPTIQWDAPEVDVVEQVGEKQRLIFIANPAD
                     REQHVTITFDMPRHLLPVWGGSAKATRDAQLTIDLDPYTIQIWEAQHD"
     gene            2857..3639
                     /locus_tag="VGT44_03155"
     CDS             2857..3639
                     /locus_tag="VGT44_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579828.1"
                     /translation="MIDPHWQVVDLDPVTWRHLGPFFEPQQYIAAAQPGEHGLFVLHD
                     QGQVLKVVDTQARTPPAGIPAQITDPEALAQELFERGTWERVHIIDRSHLAWVAQQAQ
                     SSSLSNLTLDAYYHLVYTLVWGRRGGYVSEPPPDGHWHGWTYTDIRSFIMGLPSPATL
                     ALGVYSDAVLSIGLVLICEGGQIRLVTTFEGLTWTNFQSGPTQQTLTALCEALDAQFA
                     PPAAVLLCTDAVFSGWLTASDKLAYLDAARENATAIWHISSS"
     gene            3716..4111
                     /locus_tag="VGT44_03160"
     CDS             3716..4111
                     /locus_tag="VGT44_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2579829.1"
                     /translation="MLCPNCQAECLENDEFCTICGSDLGGTSTSLVPTRSHANLPTIL
                     RNPQLPRLAAGVGAVAFGVGLALLRRNIAGRVAKPALMAAPKLIASPAFDGLRESLMQ
                     QQARPIRLPKGYEIEETAIYFSRVIRRKK"
     gene            4132..5091
                     /locus_tag="VGT44_03165"
     CDS             4132..5091
                     /locus_tag="VGT44_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEV2579830.1"
                     /translation="MALILREDDVMALLQMADTIGVIEKAFAALGEGKATNHTRIRFK
                     ENSGIMHLLAASIPSLGIMGHKTYTIFRSGMRYVVMLYSASEGDLLAIIEADWLGGMR
                     TGATSALATAYLARHAAATVGLIGAGRQATLQLLGVCQVRQIEQVFVYSRRLYEREQF
                     CKQMSQRLNIDVRPVNSSREAIEHADIVITATSSPEPVFPGDWLQPGTHINAIGSNWS
                     NRREIDQAALLRSDLVVTDSLEQALSEAGDLLIPARAGHFDLDRVHELASIIVGSAPR
                     RQSEEDITLYKGLGIALEDIATASLVYRLAIDQGRGENINILP"
BASE COUNT         1090 a         1510 c         1431 g         1180 t
ORIGIN      
        1 atgggcatcc ctgccaaacg gcaatggagc tggctgccat ccttcgccgt gtgctgcgtg
       61 gtggcaaccg ttgtcctcat accgttgctc gctactctgt taggtgggct gcgaacgaat
      121 ggcgaactct taacagagcc gttcagcatt ccaaaaacgt tacattggga aaattttggc
      181 cttgtcctgg cgagtgatag cccgttttgg ggcgaaatcg ttaatagtct tgtgacggtc
      241 acaggcacgg tcgtcctgtt gctatttgcg gcctgcccgg ccgcttttgt gctggcccgt
      301 atctcctttc gagggcgcga agtggttttt aacttcttcc tctttgggct gttattccct
      361 ctgacggttg ctgtgttgcc gctgtatatc accattcggc aatttggcct gctcgattcg
      421 ttgtggggcg tcattttacc ccaggccgcc ttcggattgc cgatcaccat cctgatcctg
      481 cgcaatttct tccgcgccat ccccatcgaa ctcgaagatg ctgccaccat cgatggggct
      541 tcgaagccga tctttttctg gcgggtgctg ctgcccttgg cgcgtccggc gatcgccgtc
      601 gtcagcatcc tggcggtcgt cagcagctgg aataacttct tgctgccgct gcttgtcttg
      661 accgaccagt cgcaatggac gctgccaatc ggagcgacgc agttccagca acaatacgcg
      721 acggattggc cctccatact tgctttcgtt acgctggcga tgattccagc aattctgatc
      781 tacctcttag ccgagcgtca aatcgtggct ggattgactg ccggggcggt gaaaggataa
      841 atttggtgac tgttaccctg actgaacaag gactcgaaat tgatgagcaa cgtgttcctg
      901 tttactccgg cagcgtacac tactggcgtc ttgaacgcga caaatggccg gtgattctcg
      961 accaggtgca atcgctcggc tttggcatgg tcgaaaccta cattccctgg tcggtccatg
     1021 aaatcgcgcc aggcacgttc gattgggggt ccgtcgatcc gcgcaaagat atcgaagctt
     1081 tttgccagct ttgcgaagct cgcggcctgt atttgatggt acgacccggc ccgctgatca
     1141 atgctgagat gacggatttc ggctttcctg aatgggtgtt gcttgatcca gaggtgcagg
     1201 cgcatacagc agtagggagc atccacctcg atgccgcatg gggtttccac ccgccaagac
     1261 cattcccggt tccctcatac gccagcccaa aattctacga ggctgttggg cggtggttcg
     1321 acgcggtatg cccggtcatc gagcggcata tggctccggg cggctgcatc gtcgcggtgc
     1381 aaagcgacaa cgagagttgc tatctgtttc acgatcaacc ctacgccacc gattacagcc
     1441 cggcctccat cgcggcttac cagcgcttcc tctgcgaacg ctacgagacg atcgtacaac
     1501 tcaacgccgt gtatggacag gacttccggg gcttcgacgc cattgcccct cctcgcgatt
     1561 gccagatcag gacagcagcc gatgttccct ggcatcgcga ttgggttgag tacaaagagc
     1621 actaccttca ccagggtgta gtgcagatcg cgcagatgct gcgcgcgcga ggaatcaagg
     1681 gcatccccat atttcacgat gtcgccttcc aatattcaac gccgcttgat atcgctcgca
     1741 tggaggccga tcctgcaatc gactgggtcg gcatgaatct ctatcgtagt cctcagaagt
     1801 atgtcggcac catccagaga atacgctacc tggcgggtac gaccaggctg ccctttgtgc
     1861 cggagttcgg cagcggcatc tggagccacc attcccagac gccgacccct gtggagcagg
     1921 aatttacaac actcgcagca ttgatgcatg gtatgcgcgc cgtcaatttt tacatgctcg
     1981 tcgaacggga acgctggcag ggcagcccga tcacccgaca tggagcgtat cgaccgggtt
     2041 acgcagaatt ctaccagcgc ctatcgattt ttctgcaacg ctatcccctc ggcacgttcc
     2101 atcgccagac gcaagtagtc gtattgctca actacgacct cggacgctat aaggcgatgg
     2161 cttccacgct gcattatgca catatcgatc tcctcaggct gcctgaagag ctagaggtcg
     2221 tcgatctgga tctcggtttc cgctgggatg tcgcccacga agctgaccaa catcgctccg
     2281 ataactggct tggaaaggtg tgcgcacaac tcaaacagca gcagttagat tacgatatcg
     2341 ctgatagcca tatcgatcca cgccggttgc aacagtatgc cataacatgc atccctacgg
     2401 tggactttat ggatgccgcc gatcagcagc aacttcttgg ctatgtcgcg gcgggtggtt
     2461 gcctcatcat cgggccaggt gtaccctatc tcgatagcaa cctgcgtcct tgttcagttt
     2521 tgaatgcagt ggttcaagaa cctggcgagg tggcgttcgg atgcgggcat atcatctggg
     2581 tgagcgccga cgcactcgct acaaccctag ctcagcgtct gccgtcaccc acgatacagt
     2641 gggatgcacc agaagtagat gtcgtcgagc aggttgggga gaagcagcgg ctcatcttca
     2701 ttgctaatcc cgcggatcgg gaacaacacg tcacgatcac ctttgatatg ccgcgccacc
     2761 tgctccctgt atggggaggg tctgcgaaag caactcgtga cgcacaattg accatcgacc
     2821 tcgatcccta cacgatccag atctgggagg cacagcatga ttgatcctca ttggcaggtc
     2881 gtcgatctcg atcctgtaac atggcgacat ctcggacctt tctttgaacc acagcagtat
     2941 attgcagccg cgcaacccgg cgagcatggt ctctttgtgc ttcacgacca gggacaggtc
     3001 ctcaaagtgg tggataccca ggccaggact ccaccagcag gtataccggc gcagattact
     3061 gatcccgaag cactcgcgca ggaactcttt gagcggggga catgggagcg agttcacatc
     3121 atcgatcgca gccatctcgc ctgggtagcg caacaggcgc agtcatcttc cctgagtaat
     3181 ctcacgctcg acgcctacta ccacctggtc tacacgctgg tttggggcag gcgtggggga
     3241 tatgtcagcg aaccgccgcc tgacggccat tggcatggct ggacctacac cgatatccgt
     3301 agtttcatca tgggattgcc ctcgcctgca accctcgcgc tcggagtcta ctcagatgcc
     3361 gttctctcca ttggccttgt cctcatctgc gagggcggac agattcgcct ggtaaccacg
     3421 tttgagggac tcacatggac caactttcaa tcggggccaa cgcagcagac gttaacggca
     3481 ctctgcgaag cccttgatgc gcagtttgcg cctccagccg cggtattgct ctgtaccgat
     3541 gctgtctttt ccgggtggct cacagcatca gataagctgg cgtacctgga tgcggcacgc
     3601 gagaacgcga cggccatctg gcatatttca tcctcctaat tgacaagaca cagcgcccat
     3661 gtcgtagtat aatgtgcagg aggaaacact acgaaaaata ggagtgaaac tggctatgct
     3721 gtgtccaaat tgccaggccg aatgcctgga aaatgatgag ttttgtacaa tatgtggaag
     3781 cgatcttggg ggaacatcaa caagcctggt gccaacgcgg agtcacgcga atcttcctac
     3841 catcctgcgt aatccccagc tgccgcgact cgccgccggt gtcggagcgg ttgctttcgg
     3901 cgttggtctg gcgttgctgc gtcgcaatat cgccggtcgc gtggcaaaac cggcccttat
     3961 ggctgcgcca aaactgattg catctccagc attcgatggc ctgagagagt ctttgatgca
     4021 gcagcaagcc aggccaatca ggctgccgaa gggctacgag atcgaggaaa ctgcgatcta
     4081 cttcagtcgc gtgatccggc gcaaaaaata aatagcggta aaggaacgac gatggcgctc
     4141 attctacgtg aagacgatgt tatggcgctg cttcaaatgg ccgacaccat cggggtgatc
     4201 gaaaaggcgt ttgcagcgct gggagaaggc aaggccacca atcacacgcg cattcgcttc
     4261 aaagagaaca gcggaattat gcacttgctg gccgcgtcga tcccctcatt gggaatcatg
     4321 ggtcacaaga cctacaccat ctttcgctct gggatgcgct acgtcgtcat gctctatagc
     4381 gcctctgagg gtgacttact ggccatcatc gaggccgatt ggttgggagg gatgcgcacc
     4441 ggcgcaacca gcgcccttgc caccgcctac cttgctcgcc acgccgccgc aacggtcggg
     4501 ctgattggcg cgggcaggca agccaccttg caattgctgg gcgtctgcca ggtgcgccag
     4561 attgagcaag tgtttgtcta tagccgccga ctgtacgagc gcgaacagtt ttgcaagcag
     4621 atgtcccagc gactaaacat cgatgttcgc cccgtcaata gttcacgcga ggcgattgaa
     4681 cacgctgata tcgtgattac ggccacttct tcacctgaac cagtcttccc cggagactgg
     4741 ttgcagcctg gcacccacat caacgccatc ggctcaaact ggtcgaaccg gcgcgagata
     4801 gaccaggccg ccctgctgcg cagcgatctc gtcgtcaccg attcgctcga acaggctctg
     4861 tcagaggccg gggatttgtt gattcccgcc agagccgggc atttcgacct ggaccgcgtc
     4921 cacgagctgg caagcatcat cgtcggctca gcgcctcgcc ggcagtccga agaagatatc
     4981 acgctctaca aagggctggg gatcgcgctc gaagacatcg caacagcaag ccttgtctac
     5041 cgcctggcga tcgatcaagg gcggggagag aatatcaata tcttgccata attgaatggg
     5101 gcgaggcggg tgatcaataa taatgctatg ccaaaacatc tacgactgat ttctataagc
     5161 gttctgctgc tctttttcct ggcgtcatgc acaaccggcg ttggctctct t
//
LOCUS       DASZAK010000187         1742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_572774, whole genome shotgun sequence.
ACCESSION   DASZAK010000187 DASZAK010000000
VERSION     DASZAK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572774"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VGT44_03170"
     CDS             complement(<1..351)
                     /locus_tag="VGT44_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HEV2579831.1"
                     /translation="MDITNNLRKVLILGAGALKIGQSGEFDYSGSQALKALREEGIAT
                     VLVNPNIATIQTSKLLADEVYFLPVTPFFVEKIIAKERPDGILLSFGGQTALNCGLEL
                     FSSGALERYGVRVLG"
     gene            complement(355..1482)
                     /gene="carA"
                     /locus_tag="VGT44_03175"
     CDS             complement(355..1482)
                     /gene="carA"
                     /locus_tag="VGT44_03175"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV2579832.1"
                     /translation="MIGHEYLMDRRQLVKEHSATWRADGVFPRVGRLVLEDGASFEGT
                     TFGYPAPAAGEVVFSTGMVGYPESFTDPSFSGQILTLTYPLIGNYGVPDRRYWEDDRM
                     HVAGLIVSDYIDLPSHAQSTMNLGTWLQREGVPALVIKDTRLLTQHIRTHGAMLGKIV
                     FDEDVPFYDPNSENLVERVSTRQVFTRGDGEPTIALIDCGAKRNITRCLLARGARVVT
                     VPWDYDLFAPEVDFDFDGIMISNGPGNPKTPQQTIATIRKALARRVPVFGICLGHQLL
                     ALAAGGDTYKLPFGHRSQNQPCILQGTKRCYITTQNHGYAVGELPPDFKPWFVNANDG
                     SNEGIMHIEYPFFSVQFHPEAMPGPVDTEWLFDYFLDRIRG"
     gene            complement(1488..>1742)
                     /locus_tag="VGT44_03180"
     CDS             complement(1488..>1742)
                     /locus_tag="VGT44_03180"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579833.1"
                     /translation="TRIQKERFASLAEYEQAMRQGFIVTPDLFQARAGRQTILMDPLP
                     RVATIDPAVDADQRALYFRSQIRNGLYIRMALLALVLGRA"
BASE COUNT          343 a          486 c          559 g          354 t
ORIGIN      
        1 tcccagtacg cgcacaccgt agcgttcaag cgcgccgctt gagaagagtt ccaggccgca
       61 gttgagcgcg gtctgcccgc cgaaggagag caagatgcca tcggggcgtt ctttggcgat
      121 gatcttttcg acaaaaaagg gcgtgaccgg caaaaaatag acctcgtcgg ccagcagctt
      181 cgaagtctgg atggtggcaa tgttgggatt gacgagaacg gtcgcgatac cctcctcgcg
      241 cagggctttg agcgcctgcg aaccagaata gtcgaactcg ccggattgcc cgatcttgag
      301 cgcgcccgcg cccaggatca gcactttgcg caggttgttt gtgatatcca attgttagcc
      361 tcgtatgcgg tccaggaagt agtcaaagag ccactcggta tccaccgggc cgggcatggc
      421 ctctggatgg aattgcaccg agaagaaggg atactcgata tgcatgatgc cctcgttgct
      481 gccatcgttg gcgttgacga accagggctt gaagtcgggc ggaagctcac cgacggcgta
      541 gccgtgattc tgcgtggtga tgtagcagcg ttttgtgcct tgcaggatgc agggctggtt
      601 ctggctgcga tgcccgaaag ggagcttgta ggtatcgccg cctgccgcca acgccagcag
      661 ttgatgcccc aggcagatgc cgaagacagg aacgcgccgg gccagcgctt tgcggatggt
      721 agcgatggtc tgctgcggcg tcttgggatt gccggggccg ttggagatca tgatgccatc
      781 gaagtcgaaa tcgacctccg gcgcaaagag gtcgtaatcc cagggcacgg tgacgacacg
      841 cgcgccgcgc gccagcaggc agcgcgtgat gttgcgcttg gcgccgcagt cgatcagcgc
      901 tatggtcggt tcgccatcgc cccgcgtaaa cacctgccgc gtcgagacac gctccacgag
      961 gttttcgctg ttgggatcgt agaacgggac atcctcgtcg aagacgattt tccccagcat
     1021 ggcgccgtgt gtgcggatat gctgggtgag caggcgggta tctttaatca ccagggcagg
     1081 cacgccttcg cgctgcagcc acgttcccag gttcatcgtg ctttgtgcgt gggatggcaa
     1141 atcgatgtag tcggagacga tcagcccggc cacgtgcata cggtcgtctt cccagtagcg
     1201 ccggtcgggg acgccgtagt tgccgatcag gggataggtc agcgtcagta tctggcccga
     1261 aaaggatgga tcggtgaagg attccgggta gccgaccatg ccggtgctga agacgacctc
     1321 tcctgctgcc ggagcagggt agccaaaagt agtcccctca aaacttgcgc catcttccaa
     1381 taccagtctt cccacgcgcg ggaagacgcc gtcggcgcgc caggttgcgg agtgttcttt
     1441 caccaattgt ctgcggtcca tgaggtattc gtgtccaatc atctatttca ggcccttcca
     1501 agcaccagcg ccagcagcgc catgcggata tagagtccgt tgcgaatttg tgaacggaag
     1561 tagagtgcgc gttgatcggc gtcaacggcg ggatcgattg tagctaccct gggcagcgga
     1621 tccatcagga tggtctgtcg gcccgcgcgc gcctggaaca gatcgggagt tacgataaag
     1681 ccctgccgca tggcctgctc gtattcagcc agggaagcaa aacgctcctt ctggatgcgt
     1741 gt
//
LOCUS       DASZAK010000188         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_5044, whole genome shotgun sequence.
ACCESSION   DASZAK010000188 DASZAK010000000
VERSION     DASZAK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5044"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VGT44_03185"
     CDS             complement(<1..550)
                     /locus_tag="VGT44_03185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579834.1"
                     /translation="MKITTPLQAKEKSLNRPSSGQARFAFSILLIINILNYADRYVLP
                     AILPKIQADLGLTPFQEGLLGSSFLLVYALATLPLGVWADRSVRKNIVALCVGIWSVA
                     TVLAGLTRNFAQLFSVRAVLGIGEAGYAPASLSLLGDFFSKEKRGRILSFWSSGTLIG
                     AAIGFTLGGLVADSFGWRYAFFI"
     gene            complement(572..2281)
                     /gene="serA"
                     /locus_tag="VGT44_03190"
     CDS             complement(572..2281)
                     /gene="serA"
                     /locus_tag="VGT44_03190"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEV2579835.1"
                     /translation="MEEELGHVEEEIRILIADRIAQEGIDLLRSQLPEAHIDIQLGLK
                     PDQLKSIIGAYTALIVRSETQVTGEILAAAKQLKIVGRAGVGVDNIDIEAATRQGIMV
                     VNSPTGNIVAAAEHTIAMLMALARHIPEASSSTKAGKWEKNRFLGVEVRNKVLGIIGL
                     GKVGTEVARRAQGLEMQVIAFDPYVAPEQARKLGVTMTSMEEVLQQADFVTLHTSLTG
                     GPQGTRGLIGAHELQLLKPGARLLNCARGGLIDEEALLNALQQNRVAGVALDVFSQEP
                     IADNAVLKQLLAHERVIATPHLGASTEEAQLGVATDVAEQIVAVLHGGFPRAAVNAPL
                     ILPETLKILQPYMHLVEQMGRLYTQLQPGPLNKIEFSCSGDIANNDLRPLQAALIKGL
                     LESVSDAHVNMINAPLLAKQWGLEIIEQKSTISTEFANLVTIRLLHTNGYSSSFAGKP
                     GSGDDYVEVVSGTVMHNEPRIVRIGRYWTEFVPEGYILFCRNPDQPGMIGRVGTILGK
                     ARVNIRHMDVGPTARAPHTRDPRRSPDTALMIISVDDPIPDWALEEIGGSGDIFGVTL
                     VKV"
     gene            complement(2301..3458)
                     /locus_tag="VGT44_03195"
     CDS             complement(2301..3458)
                     /locus_tag="VGT44_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEV2579836.1"
                     /translation="MTIHERKAIPIPQVEPRQPVPVQNLRIPGPTPVPAEILKEMTQP
                     LINHRGPEFSAILRRVTARLQYFFQTNSPVLTFAASGTGGQEAAVANLFSPGDHVAAI
                     TIGSFGNRLATIAERYGLQVTRIEFPWGEAADPVTVETRLKSLAPYRAVLMTHNETST
                     GVTNDLETLAALVSRLNPDALTVVDAVSSLGCVPLEMDAWGIDVVFTSSQKGWMTPPG
                     LMMIAASPRAWEANKTAKLPRFYFDWELARKYLEKWEHPTTPPVSLFYALDLALEKML
                     EEGREAIFERHQRIGDYVRWRAKEMGLGLLADHKNASNTITAVLTPEGVDAKALLKVL
                     REEDHVVFAGGQQHLEGKIFRVGHLGFFEEVDLVEAMDAMEMRLHTFGFNA"
BASE COUNT          847 a         1046 c          993 g          870 t
ORIGIN      
        1 caataaagaa tgcataacgc cagccaaaag agtccgcgac gaggccgcca agggtaaagc
       61 cgatagcagc gccaatcaat gtgcctgacg accagaacga caggatgcgg cctcgtttct
      121 ctttggaaaa gaaatcacca agcagcgaga gcgaagccgg ggcataacct gcttcaccaa
      181 tacccaacac agcacgaaca ctgaacaatt gtgcgaaatt gcgcgtgagg cctgccagga
      241 cggtcgctac actccagatt cccacgcaca gcgctacgat attcttgcgt acgctcctgt
      301 ccgcccaaac ccccaggggc aatgtggcaa gcgcgtacac gagcagaaat gatgatccaa
      361 gcagcccttc ttgaaacggt gtcagcccca ggtccgcctg aattttcggc aatatagccg
      421 ggagtacgta gcgatcagca tagttgagga tgttaatgat aagcaatata gaaaaagcaa
      481 aacgagcctg cccgctactc ggcctgttga gagatttctc tttggcctgc aatggagtgg
      541 ttatcttcat tgtgacatac tttctcccac tctacacctt caccaatgtg acgccaaaaa
      601 tgtcccctga gccaccgatc tcttcaagcg cccaatcggg aatggggtca tctacggaga
      661 taatcatcag tgcagtatca ggagaacgcc tggggtccct ggtatgtggc gctcgcgcag
      721 tgggtcctac gtccatgtgt cgaatattta cccgggcttt acccaggatg gtgccgacgc
      781 gcccaatcat gccgggttga tcagggttgc ggcagaagag gatgtagcct tccggcacaa
      841 attcagtcca atagcggccg atacgaacga tacgcggttc attatgcatg accgttccac
      901 ttaccacctc aacatagtcg tcgccagagc ctggttttcc cgcgaaggag gagctatagc
      961 cattggtatg caaaaggcgt atggtgacga ggttggcaaa ctcggtcgat atggtcgatt
     1021 tctgttcgat gatctccaat ccccattgtt ttgccagcaa cggcgcattg atcatgttga
     1081 catgcgcatc tgaaaccgat tcgagcagcc ctttgatgag cgctgcctgt aaagggcgca
     1141 aatcgttatt cgcgatgtca ccgctgcatg agaattcgat cttgttaagc ggccccggtt
     1201 gtagttgtgt gtacaaacgt cccatctgct caacaaggtg catataaggc tgcaatattt
     1261 taagggtctc ggggagaatg agtggcgcgt tgaccgccgc tcgcggaaag ccgccgtgca
     1321 agacggcgac aatttgttca gccacatccg tagcgacccc cagttgcgcc tcttccgttg
     1381 aggcccctag gtgtggtgtt gcgatcaccc gctcgtgtgc gagcagttgc ttcagtaccg
     1441 cattatccgc aattggttcc tggctgaaga catcgagcgc gactcccgcc acgcgattct
     1501 gctgcagcgc gttgagcaag gcttcttcat cgatcagtcc acctcgtgcg cagttgagca
     1561 atcgcgctcc gggtttgagc agttgtaatt cgtgcgcgcc gataagcccg cgagttcctt
     1621 gcgggcctcc ggtgagtgag gtatgcagcg tcacaaaatc ggcctgttga agcacttctt
     1681 ccatactcgt catggtgaca ccgagcttgc gagcttgttc cggggcgaca tagggatcga
     1741 acgcgatcac ctgcatttcc agtccctggg cgcgtcgcgc tacttcggtg ccgacctttc
     1801 ccaggccaat aatgccgagt actttgttac gtacctcgac tcccaggaag cgattctttt
     1861 cccatttccc tgccttggtg ctgctgctgg cttcaggaat gtgacgcgct aatgccatca
     1921 gcatggcgat ggtatgttcc gccgccgcta caatgttgcc cgtaggcgaa ttgacgacca
     1981 tgataccctg gcgcgttgct gcttcgatat cgatattatc gacgccaact ccggcgcgtc
     2041 ccacgatctt gagctgtttg gccgctgcca gtatctcgcc cgtgacctgg gtttcgctgc
     2101 gcacaatcaa cgccgtgtag gcgccgatga tgctcttgag ttgatcaggc ttgaggccga
     2161 gctgtatatc tatatgcgct tctggaagct gcgagcgcag gagatcgatg ccctcctgcg
     2221 cgatgcgatc agcgataagt atcctgattt cttcttccac gtggcccagt tcctcttcca
     2281 tcgttactgg ttcctctcta ttacgcgtta aaaccgaatg tgtgcaggcg catttccata
     2341 gcatccatgg cctcgaccag gtccacttcc tcgaagaaac caagatgccc gacacggaaa
     2401 atcttcccct ccaggtgttg ctgtccgccg gcaaagacca cgtgatcctc ttcgcgcagc
     2461 accttcagca gcgctttcgc atcgacccct tcgggtgtga gtacggctgt aatggtattc
     2521 gaggcgtttt tatggtccgc gagcaatccc aaccccatct ctttcgcacg ccagcgcacg
     2581 taatcgccta tgcgttgatg tcgttcgaag atggcctctc gtccctcctc cagcatcttc
     2641 tcgagcgcca ggtccagagc ataaaacagc gagacaggcg gtgtagttgg atgctcccat
     2701 ttctccaggt acttacgagc cagttcccag tcaaaataga aacgaggcag cttcgcagtt
     2761 ttattggctt cccatgcgcg aggactggcg gcaatcatca tcagcccagg aggcgtcatc
     2821 caccctttct gtgagctcgt gaagacgaca tcgatacccc aggcgtccat ttcaagggga
     2881 acgcagccaa gtgaacttac cgcatcgaca acagtgagcg catcggggtt gagacgacta
     2941 accagggctg ccagcgtttc caggtcattc gtcacgcctg tagaggtttc gttatgcgtc
     3001 atgagcactg cccggtaagg ggcaagagat ttcaggcgtg tttcaaccgt cacaggatca
     3061 gcggcctctc cccagggaaa ttcgatgcgg gttacctgca atccatatcg ttcagcgatt
     3121 gtcgccaggc gatttccaaa agagccaatc gtgatcgccg caacatgatc gcccggtgag
     3181 aagaggttag cgaccgctgc ctcctgtcct ccagtaccgg aagctgcgaa ggtcagcacc
     3241 ggtgagtttg tctggaagaa gtattgcaag cgtgcagtga cgcgccgcag gatggcggag
     3301 aattcagggc cgcgatgatt aatcaaaggc tgagtcattt ctttcaagat ctcagcaggt
     3361 acaggtgttg ggccgggaat gcgcaggttc tgtactggaa caggttgacg aggctctacc
     3421 tgtgggatag gaatagcttt acgttcatga attgtcatgt gttactccat ctatagcggc
     3481 ctggatggcc agtctacgtt caattgtact tgtgcctcat tgcaataaga gacggcggac
     3541 tactacgtag tatccgccgc gctgctcgat aactcactat gaaagtatag cataggtcgc
     3601 cattatagga aacaccgatt tttgtcgcac ttgtcgcgtt tgtatcactt gtatcgccca
     3661 ggaacgcgaa tagaaactat catcagcaat aatgccagga taagcaaaag cacaatctgc
     3721 gcaagtgccc ccggctgagc cgaaacgaac agggca
//
LOCUS       DASZAK010000189         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_608799, whole genome shotgun sequence.
ACCESSION   DASZAK010000189 DASZAK010000000
VERSION     DASZAK010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608799"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..>2474
                     /gene="clpB"
                     /locus_tag="VGT44_03200"
     CDS             189..>2474
                     /gene="clpB"
                     /locus_tag="VGT44_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEV2579837.1"
                     /translation="MKIEQFTDKAREAISEANELAKQHNHSQIEAEHLLAALLAQEGG
                     VVQQIIQKVGGNLATAQRMVTTELERLPRVYGGSEPGISPRLRRLLENAWQEMGKFHD
                     EYLSVEHMLLAMFDAGDEPVQRALKAAGLTRDIVLKALTSIRGAQRVTDQNPEGKYQA
                     LEKYGRNLTQLAEQGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVRGDVPKTLAGKQVVTLDLGALVAGAKYRGEFEERLKAVLKEVTSSEGNIILF
                     IDELHTLVGAGAAEGAMDASNMLKPMLARGELHCIGATTLDEYRKHIEKDAALERRFQ
                     PVMVDQPSVEDTISILRGLKQRYEVHHGVRIQDSALVAAAVLSNRYITDRFLPDKAID
                     LVDEAASRRRVELDSTPSEIDALDRRIRQLQVEQEALKKETDPASRERRAKVEQEIAN
                     LSEQLHTLRLSLESEREPVEAIRGLKKELEQAQLELEKAEREYNLDLQARLRYGTIPD
                     LERRIQEMESKLTSIKGARMLKEEVDAEDIAEIVSKWTHIPVTRLMEGEVQKLLIMED
                     RLRERVVGQELALQTVSDAIRRSRAGLQDPNRPLASFIFLGPTGVGKTELARALAEFL
                     FDDERALVRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTETVRRRPYSVVLFDEIE
                     KAAPEVFNVLLQILDDGRLTDGQGRTVDFKNTVIIMTSNVGTAWLHEFDGMDEDEIQR
                     VVRQRLREEGFRPEFINRIDEV"
BASE COUNT          608 a          665 c          738 g          463 t
ORIGIN      
        1 ccacgttttt tcttgtcaag agtgtaacat ataagctata tcctgtcgag gaggcaattt
       61 tctcacctct aataataaaa tgcagccagc gaaaacttga tagcaataca ctcatacttt
      121 cttgcagtag ctgactcaat atgctatact aattaccggc aaagactatt tacaatgggg
      181 agttttttat gaaaattgag cagttcacag acaaagctcg tgaagccatc agcgaggcca
      241 atgaactggc taagcaacac aaccacagcc agatcgaggc tgagcacctg ctggccgctc
      301 ttctagcgca ggaaggcggg gtagtgcagc aaattatcca gaaggtggga ggcaacctgg
      361 caacggcgca gcgcatggtc actaccgagc ttgaacgtct gccccgcgtc tatggcggta
      421 gtgagccagg catctcgcca cggctgcgca ggctcctcga aaatgcctgg caggaaatgg
      481 gcaagttcca tgatgaatac ctcagcgttg agcatatgct gcttgccatg ttcgacgccg
      541 gtgatgagcc ggtgcagcgt gccctgaaag ctgccggcct cacccgtgac attgtgctga
      601 aagccctcac atccatacgc ggagcgcagc gtgtgaccga tcagaatccc gaggggaaat
      661 accaggcatt agagaaatat ggccgcaacc ttacgcaact ggcggagcaa ggcaaactag
      721 acccggtgat tgggcgcgac gaagagatcc ggcgcgtcat ccaggtactg agccgccgca
      781 ccaagaataa ccccgtattg atcggtgaac caggtgtagg gaaaaccgcc atcgtcgagg
      841 gattggcgca gcgcattgtg cgcggcgacg tgccgaaaac actggccggc aaacaggttg
      901 tcacgctcga cctgggcgcg ctagtagcag gagccaagta ccggggcgag ttcgaagagc
      961 gcttgaaggc tgtgctgaaa gaagtgacaa gctcggaggg gaacatcatc ctcttcatcg
     1021 acgaattgca taccctggtg ggcgcaggcg ctgccgaagg cgcgatggat gcctcaaaca
     1081 tgttgaagcc catgctggcg cgcggcgaac tgcactgcat aggcgcaacc acgctggatg
     1141 agtaccgcaa gcacatcgag aaggatgccg ccctcgagcg ccgtttccag cccgtgatgg
     1201 tcgatcagcc ctcggtcgag gacactatca gtatcctgcg cggactgaaa caacgctacg
     1261 aggtgcatca tggcgtgcgc atacaggata gcgcgctggt agccgccgca gtgctctcca
     1321 accgctatat taccgaccgt tttctgccgg ataaggctat cgacctggtt gatgaagccg
     1381 ccagcaggcg gcgggtggaa ctggacagca ctcccagcga gatcgacgcg ctggaccgcc
     1441 gtatccgcca gttgcaggtg gaacaagagg cattgaaaaa agaaaccgac ccggccagtc
     1501 gcgagcggcg tgctaaagta gagcaagaga ttgctaacct gagcgagcaa ctgcacactc
     1561 tacgcctctc tctggaaagt gaacgcgaac cggttgaggc gattcgcggg ctgaagaaag
     1621 agctggagca agcgcagctc gaactggaga aggctgaacg cgagtacaac ctcgacttac
     1681 aggcacgact gcgttacggc actattcccg acctggagcg acgtatccag gaaatggaat
     1741 caaagctgac gagcatcaag ggcgcgcgta tgctcaaaga agaggtcgat gccgaagata
     1801 ttgccgagat cgtctcgaag tggacacata ttcccgtcac caggttgatg gagggtgaag
     1861 tacagaagtt gctcataatg gaggatcgcc tgcgcgagcg cgtcgttggg caggagctgg
     1921 ccctgcagac cgtctccgac gccattcggc gttcgcgcgc cggcctgcaa gacccgaatc
     1981 gtccgttggc cagcttcatc ttcctgggtc caacgggtgt agggaaaact gaactggcgc
     2041 gagcgttggc agagttcctg ttcgacgatg aacgcgctct agtccgtatt gacatgtcag
     2101 agtacatgga gaagcatagt gtctcgcgct tgatcggtgc tcctcccggg tacgttggct
     2161 acgaagaggg cggccagctt acagagactg tgcgccgtcg cccctacagc gttgtactct
     2221 tcgatgagat cgaaaaagcc gcgccggaag tcttcaacgt gctactacaa atactcgacg
     2281 atggtcgcct gacggatggt cagggacgca cggtcgattt caagaacacc gtcattatca
     2341 tgacgagcaa cgtcggcacc gcgtggctac acgagttcga tggcatggat gaggacgaga
     2401 tacagcgcgt ggtgcgccag cgcctgcggg aagagggctt ccgtccagag ttcatcaacc
     2461 gtattgacga ggtg
//
LOCUS       DASZAK010000190         7804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_181558, whole genome shotgun sequence.
ACCESSION   DASZAK010000190 DASZAK010000000
VERSION     DASZAK010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7804
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181558"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="VGT44_03205"
     CDS             complement(<1..1391)
                     /locus_tag="VGT44_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV2579838.1"
                     /translation="MNSKTERPPRRPANARSAKTRSNKTNRYSRQTARGIEAKRDGKP
                     LIFGWGKHLSHSDKMKVQRRSVWAFTGLIILVMVAVLVGSWINNNIIIPGLPITTVNS
                     HQIPQSQFRKMVALKTLVEYNKLNGRNGLNAQHSSLETQNAAQLRTINSINATIASLN
                     TRIAKLPKGPSAQRTDLNNQLKAQQTALATAQTKESSLASQINVLTTNTIPLEQQLFT
                     ESQIATDSATWLQDDELIREWLANQSAALQNKINPTAGAVQQALNALAANVPTTTTYN
                     SLLSQMSVSDSDMHDMMTISLRRDNMQTYLASLIKSPAYQVNARSMTLATMANAQTML
                     KQLQANDATDFATLAKQKSQDNTSAPQGGNLGWLIRGQYAQNEGSGTVDDWLFDPQRF
                     VGELSPILQENGTFRILQIMSIDPSRKIDATTLQTAQNNALSNWLLELKAEPTTHVSQ
                     PDSNMLLDPNNLPP"
     gene            complement(1528..1821)
                     /gene="rpsR"
                     /locus_tag="VGT44_03210"
     CDS             complement(1528..1821)
                     /gene="rpsR"
                     /locus_tag="VGT44_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV2579839.1"
                     /translation="MQRRSEGSSGGSRSRSSSRFERTPRRKICQFCHDHVREIDYKDA
                     SRFLKRFISDRGKIEPRRKTGTCAKHQRRLSTALKQARFMALLPYTAEHMRNM"
     gene            complement(1868..2281)
                     /locus_tag="VGT44_03215"
     CDS             complement(1868..2281)
                     /locus_tag="VGT44_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV2579840.1"
                     /translation="MNKIILIGHLGRDPELQVTPDGTPVTKFSLAVNRYSRTNTGERK
                     EETDWFNVTAWRQLAETCEKYLHKGSKVYIEGRLTQRKYTDRDGIQRTSIDVSMTEME
                     MLTPKSASTGSGGYAGNSSSDNDDPFLPDFPDDAS"
     gene            complement(2408..2881)
                     /gene="rpsF"
                     /locus_tag="VGT44_03220"
     CDS             complement(2408..2881)
                     /gene="rpsF"
                     /locus_tag="VGT44_03220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV2579841.1"
                     /translation="MKRDYELGFILNPEVNEEQTGAVLTRISQVVSNHDGQIVRVNQW
                     GRRRMAYPIDHHRDGHYIFMDMILTPETVAELDRTLKVTEDVLRHMVVRRDPKAAQKE
                     REEIEARAAAQEQAASAPPEAETPQESEDSLPVELTPEEIEYVPPAIEEEESVEA"
     gene            2959..3171
                     /locus_tag="VGT44_03225"
     CDS             2959..3171
                     /locus_tag="VGT44_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579842.1"
                     /translation="MTNPCAFSPVEEMLFAFKYAPAIVLVFSPGEKAPRYYNIVHFGW
                     QCFDDHRSSILPAIQKNGNPAAVAVY"
     gene            3233..5104
                     /locus_tag="VGT44_03230"
     CDS             3233..5104
                     /locus_tag="VGT44_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579843.1"
                     /translation="MQPDDQFYNSARANGSPDRSSRSLRARRTSPALPGITMPTAPIV
                     PSVSTTPVSSTGRSIIPSTPSPRGGGQAPHRQQPARRAAWKLLASGILLALVTLLLYP
                     LLAGALPSGNAAGKSALAVLTALTGLFLWIPRLYWTAWPPATSAISHLPMFDVSQPGG
                     FANLLLLGLVIAGGLYLMAVRVARNVGRERLTDRETHRLFWIIFTLAALFALLYLFAP
                     AVMSQDALLYGLYGRMVSLYHVNPYVAPVTIPPGDVLHGVLVGQIGRPPFGPVWMDVS
                     LAVALTAQGSAATILFDFRLLALVIHLVNALLLWNVLARLRPEARFTGMVLYAWNPAV
                     LLLGIGEMHLDLVVIFFVLLAILFFQRRALVLCWVCLLLSALIQPLSLLLFPLFIRLM
                     WKATRSMSRRAVWWLMLIGVSALVVALAYAPYYAGWSVAGIAGQLRLAFAPGVALDSL
                     DAAIQHLRFGVVPVVAWLAAPLTWTIFAALVVGCLLLLGLWLTENLEYALLFTAWVCL
                     AILVISPSYLAWSALLPLALAICSASARTTLFALLLAAGALLSYYFNLNAAPWDGQGL
                     VTLGLPVLIWGWSLFFSSTMHMTRAGAEARGQSSGQLPAIRPTRGPRFSRPSWPGRR"
     gene            5214..5429
                     /locus_tag="VGT44_03235"
     CDS             5214..5429
                     /locus_tag="VGT44_03235"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEV2579844.1"
                     /translation="MNKKPPPTVWESLALMGQLGFTIAIPLALGAWGGNYLDGQTNHK
                     PLFLMLGLLLGLIVGIYGAYRLFARFF"
     gene            5526..6422
                     /gene="atpB"
                     /locus_tag="VGT44_03240"
     CDS             5526..6422
                     /gene="atpB"
                     /locus_tag="VGT44_03240"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV2579845.1"
                     /translation="MNIWQLQPIQIQPDVIFTLFGLPVTNTLLCTWITIVLLVIVAYF
                     GTRRRDLIPSGMQNFVEWGIESILRLVQSVVGEKKGRQFFPLVATFFFFILFANLFDV
                     LPGIDTIGRINITAVQAAHLAVPSGPFLFGDLSNKIVPFFRPPTSDLNLTFSMALVSV
                     VVTQVFGFMYLGAGPHLSKYFQFRAIKEHGVQGGIEFFVGIIELVTEVSRLLSFAFRL
                     FGNIFAGGAVLAVFAWLLPGITDVVFIPLELFVAFVQALVFSLLTLVFLQMGTTSHEH
                     TESEHEAESEYEAAKEAAAVGR"
     gene            6518..6748
                     /gene="atpE"
                     /locus_tag="VGT44_03245"
     CDS             6518..6748
                     /gene="atpE"
                     /locus_tag="VGT44_03245"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEV2579846.1"
                     /translation="MTGSINALAVALAIGLGAIGPGIGIGIAAGQAFNAIGRNPEAEP
                     LVRINMFIGLAFAEAISIYALVISFVLSGSVK"
     gene            6836..7360
                     /gene="atpF"
                     /locus_tag="VGT44_03250"
     CDS             6836..7360
                     /gene="atpF"
                     /locus_tag="VGT44_03250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV2579847.1"
                     /translation="MHIQVFADLLGSLGINLLAFLSQLISFGIVFLILWRWALPVILR
                     TLEKRQSVIREGIENAERARRDLQEANVRAEQILLDARRQAQETIALAAKAAEREANR
                     IQEEAQVRAHQVEQQQIARIRQEAAQAREELSRLVVNLSINAAGKVISKSVDTNDNRR
                     LVEEFVTASQTKES"
     gene            7360..>7804
                     /gene="atpH"
                     /locus_tag="VGT44_03255"
     CDS             7360..>7804
                     /gene="atpH"
                     /locus_tag="VGT44_03255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEV2579848.1"
                     /translation="MLKGAIGRRYAGAIFDIAMKQNTIDRTLDDVREFARLFSHRTLA
                     YLLNEPKIPTQRKETAIRQALASKVLPTSLNLALLVVQRELVEVMPAIARELEQLVLN
                     YKNQAYAEVTTAMPMDQQQQSEVRQALEQQTGKTILMQTKVSPDIL"
BASE COUNT         1399 a         2170 c         2268 g         1967 t
ORIGIN      
        1 ggcggcaggt tgttgggatc gagcaacatg ttggaatctg gctgcgacac gtgagtcgtc
       61 ggctccgctt tgagttccag gagccagttt gacagggcgt tgttctgcgc cgtttgcaat
      121 gttgtggcgt cgatcttgcg cgatgggtcg atgctcatga tttgcaggat gcggaacgtg
      181 ccgttttctt gcaagatcgg gctgagttcg ccgacaaagc gctgcgggtc gaacagccag
      241 tcgtcaaccg taccgctgcc ctcgttttgc gcgtactggc cgcgaatcag ccagcccagg
      301 ttgccgccct gcggtgcgct tgtgttatcc tgcgacttct gcttcgcgag tgtagcgaag
      361 tctgtggcgt cgttggcctg caactgctta agcatcgttt gcgcgttggc catcgtagca
      421 agggtcatcg agcgcgcgtt tacctggtag gccggcgact ttatcagcga cgccaggtag
      481 gtttgcatat tgtcgcggcg caacgagatg gtcatcatat cgtgcatatc gctatcggat
      541 acgctcatct gactgagcag gctgttatag gtcgtcgttg tgggtacatt ggcagccagg
      601 gcgttgaggg cctgttgaac ggcgccggcg gtcgggttga tcttgttttg cagcgcggcg
      661 ctctggttgg caagccattc acggatgagt tcgtcgtcct gcagccacgt ggcgctgtcg
      721 gtggcgatct gcgattcggt gaataactgc tgctcaagcg gaatagtgtt cgtcgtcaag
      781 acgttgattt gcgatgccag gctgctctct ttggtctgtg ctgtggcaag cgcggtttgc
      841 tgggctttca actggttgtt aaggtcagtg cgctgcgcgc ttggaccctt tggcagtttg
      901 gcaatcctgg tgttcaacga cgcaatcgtc gcattgatac tgttaatggt tctgagttgc
      961 gcggcgttct gcgtttccag gctgctatgc tgggcgttca ggccattacg gccattgagt
     1021 ttgttatatt caaccagggt tttcagcgca accattttgc ggaattgcga ttgtggaatc
     1081 tggtggctgt tgacggttgt gatgggaagt cccggtataa tgatgttgtt gttgatccaa
     1141 ctgccgacca gcacggccac catgacgagg ataatcaatc ctgtgaacgc ccagacactg
     1201 cggcgctgca ccttcatttt gtcgctatgt gaaaggtgtt tgccccagcc gaaaataagc
     1261 ggtttgccgt cgcgctttgc ttcgatgcct cgggcggtct gacgagaata tctattcgtt
     1321 ttattggagc gcgttttcgc cgagcgcgca ttcgcgggtc ggcgtggtgg tcgctctgtc
     1381 ttactattca taggtttccc ttcctgctgc aaacaaatag cccggcattc acagaacaca
     1441 caaacacagc gaaggctgat gcggcgctcc ctttatagat ataactacgc aggagccagc
     1501 atcatcttca ctgtgttatg cattgctcta catattgcgc atgtgttccg cagtgtaggg
     1561 cagcagcgcc atgaaccgcg cctgtttcag ggcggtactc aagcggcgct ggtgtttcgc
     1621 gcatgttccc gttttgcggc gtggctcgat cttgccgcga tcagaaatga aacgcttcaa
     1681 gaatcggctg gcgtccttgt agtcgatttc acgcacgtga tcgtggcaga actggcatat
     1741 tttgcggcgt ggcgtgcgct caaaccgcga ggatgaacgc gagcgcgagc cgcctgatga
     1801 accttcactt cttctttgca tgattgcttc tccttgttag ccccggcagg ttgccgcgga
     1861 ccttatttta ggaggcatca tccgggaaat cgggcaggaa aggatcgtca ttgtcgctgc
     1921 tactgttacc tgcatagcct ccgctacccg tcgaggcgga cttcggcgtg agcatctcca
     1981 tttcggtcat ggacacatcg attgaggtgc gctgaatacc gtctctgtcg gtatatttgc
     2041 gctgcgtgag gcgaccttcg atgtacacct ttgaaccttt atgcaggtac ttctcgcatg
     2101 tttcggcgag ttgcctccag gcagtaacgt tgaaccagtc ggtttcttcc ttgcgttcac
     2161 cagtatttgt gcggctgtac cggtttaccg ccaggctgaa ctttgtgaca ggagtgccat
     2221 ccggtgtgac ttgcaattcg gggtctcgtc caagatgccc aattaggatg attttgttca
     2281 ttgtgtttcc aatcctttca accgtttgtc gttatgacga ctccttcact gaaaccttgt
     2341 agtcttgctg aaccgcgcaa gccgtgcagt cttcctttgt agtatctacc cgaaccagac
     2401 gacgctccta tgcttcaacg ctttcttctt cttcaatagc aggcggaacg tattcgattt
     2461 cctccggggt tagctcgaca ggcagcgagt cttcgctttc ttgcggtgtc tccgcttcgg
     2521 gcggggcgct ggcggcctgt tcctgcgcgg ctgcgcgagc ttcgatctcc tcgcgctcct
     2581 tctgtgcagc ctttgggtcg cgccggacga ccatgtgacg cagtacgtct tcagtcacct
     2641 tgagtgtgcg atccagttcg gcaacagtct caggagtgag aatcatgtcc ataaaaatgt
     2701 agtgaccatc gcgatggtgg tcgatgggat aggccatgcg ccgtctcccc cattgattga
     2761 ctcgcacgat ctgaccgtca tggttcgaga cgacctgcga gatgcgcgtt aagaccgcgc
     2821 cagtttgctc ttcgttgact tcgggattga gaataaagcc taattcgtaa tctctcttca
     2881 cgaagccacc tccttcctct gggaatgctt ccggtcaatg gccctcgagc agaggggcgc
     2941 tagccggagc aggtgttttt gactaatcca tgcgcttttt ctcctgttga ggagatgctt
     3001 tttgctttta agtatgcacc tgccatcgta ttggttttta gcccaggtga aaaagcacct
     3061 cgctattata acattgttca tttcggctgg caatgctttg atgaccatcg atcatcaatt
     3121 cttcctgcca tacaaaaaaa cggaaatcct gctgccgtcg cggtttattg acatagatta
     3181 atctttaacg catactgtaa gtcgtcaacc atcatgcatt ggacggggaa ttatgcagcc
     3241 tgacgaccag ttttacaatt cagcaagggc gaatggctct ccagatcgct cgtctcgctc
     3301 gctgcgcgcg cgccggacga gtcccgcgct gcccggtatc acgatgccaa ccgctccaat
     3361 cgtcccgtcc gtctcgacca cgcctgtctc atccaccggc aggtctatca tcccctcgac
     3421 gccgtcgccg cgaggtggcg gccaggcgcc ccatcgccag caacccgccc ggcgcgccgc
     3481 gtggaagctg ctggcgagcg gcattttgct tgcgctggtg acgctcctgc tctaccccct
     3541 tctggcaggc gcgcttccct ccggcaacgc ggctgggaag tcagcgctgg cggtcctgac
     3601 cgctctgacc ggcctgtttc tctggattcc gcgcctgtac tggacggcat ggccgcccgc
     3661 gacgagcgcc atcagtcacc tgcccatgtt tgatgtgagc cagccaggcg gcttcgcgaa
     3721 tctgttgctg ctgggtttgg ttatagcggg tggcctttac ctgatggccg tgcgcgtcgc
     3781 ccgcaatgtg ggacgagagc ggctgactga tagggagacg caccggctgt tctggatcat
     3841 cttcacgctg gccgcgctct tcgcgctgtt gtacctcttc gctccagcag taatgtcgca
     3901 ggacgcgctg ctttacggcc tgtatgggcg catggtctcg ctgtaccacg tcaatcctta
     3961 cgtcgcgccg gtcacgatac cgcctggcga cgtgctgcat ggtgtgctgg tcggccagat
     4021 aggtcgccct ccctttggcc cggtatggat ggacgtttct ctggccgttg cgctcaccgc
     4081 gcagggaagc gctgcgacga tcctgttcga ctttcgcctg ctagcgctgg tgatacacct
     4141 ggtgaatgcg ctgctgctat ggaacgtact ggcgcgcttg cgaccggagg cgcgcttcac
     4201 cggcatggta ctctacgcct ggaatcctgc cgtgctgctt ttgggtatcg gcgagatgca
     4261 tctcgacctg gtggtgatct tctttgtctt gctggctatc ctgtttttcc agcgccgcgc
     4321 gcttgtcctg tgttgggtct gcctgctctt atcggcattg attcagccat tgagtctgct
     4381 gctcttccct ctgttcatcc gtctgatgtg gaaggcaacg cgcagcatga gtcggcgtgc
     4441 cgtgtggtgg ttgatgctga tcggcgtatc ggcgctagtc gtggcgctgg cctatgcgcc
     4501 gtattacgcg ggctggagcg tggccggtat cgccgggcaa ctgcgcctcg ccttcgcgcc
     4561 tggtgttgcc cttgactcgc tcgacgcggc catccagcat cttcgcttcg gcgtcgttcc
     4621 cgtcgtcgcc tggctcgcgg ccccgttgac ctggacgatc tttgccgcgc tggtggtcgg
     4681 ctgcctgttg ctgcttggcc tgtggctcac ggaaaatctc gaatacgcgc tgctcttcac
     4741 cgcctgggtg tgcctggcca tactggtaat ctctccatcc tacctggcgt ggtcggcgct
     4801 tctgccgctc gcgctcgcca tttgcagcgc cagcgcgcgc acgaccctgt tcgcgctgct
     4861 gctggcggca ggcgcgctgc tctcctacta tttcaacttg aacgccgctc cctgggatgg
     4921 gcaggggctg gttacactgg gcctgcctgt gctgatctgg ggctggtcgc tcttctttag
     4981 ctctaccatg catatgacgc gcgctggagc cgaggcgcgc gggcagagtt cgggacaact
     5041 gccagcaatc agacccacgc gcggtccacg cttctcgcga ccctcctggc ctggccgcag
     5101 ataggctgct gattacccac ccgtagcctt gtatcacctg ttgcattagt tctcttttgt
     5161 gtgctataat ccgcatggat ataatagaag agaagacaga tggggtaact cggatgaaca
     5221 aaaagccgcc gcccactgtt tgggaatccc tggcgttgat ggggcaactc ggcttcacca
     5281 tcgcgattcc cttagctcta ggggcctggg ggggtaacta cctcgatggc cagaccaatc
     5341 acaaaccatt atttttaatg ctcgggctgc tgcttggcct gattgtgggc atctacgggg
     5401 cgtaccgttt atttgctcgg tttttttagc ggacgcgagg catcgggtcc ctacaaagga
     5461 tagacggaac tttccagaca gaacaaccag ggtgggcaga gcgcggtata taagggagga
     5521 accgcgtgaa catttggcaa ctgcaaccaa tccagattca gccagacgtg attttcactc
     5581 tgtttggtct gccggtcacc aacacgttgc tttgcacctg gattaccatt gttctgctgg
     5641 tgatcgtggc atactttggc acacgccggc gcgacctcat cccatccggt atgcagaact
     5701 tcgtcgaatg ggggattgag agcatcctcc ggctggtgca gagtgtggta ggagagaaga
     5761 agggtcggca gtttttccca ttggtcgcga cctttttctt cttcatcctg tttgctaacc
     5821 tgttcgatgt cctgcctggc atcgatacga ttggtcggat caacatcacg gctgttcaag
     5881 ccgcgcacct ggcggttcca tcggggccct tcctgttcgg tgatctttcc aacaaaatcg
     5941 ttcccttctt tcgcccgcct accagcgatc tgaacctgac gttctcgatg gcgctagtct
     6001 cggtggtagt gacgcaggtt tttggcttca tgtacctggg cgcgggtccg catctgagca
     6061 agtacttcca gttcagggcg atcaaagagc atggagtgca ggggggtatc gagttcttcg
     6121 ttggaattat cgagctggtg acagaggttt cccgcctgct ctcattcgcc ttccgtctct
     6181 tcgggaacat cttcgcgggc ggcgcggtgc tggccgtgtt cgcgtggttg ctgcccggca
     6241 tcaccgacgt agtcttcatc ccgcttgaat tgttcgtggc ctttgtgcaa gcgctggtct
     6301 tctcgctgct gaccctggtc ttcctgcaaa tggggacaac ctcgcacgaa catacggaaa
     6361 gcgaacacga agccgagtca gagtacgagg ccgctaagga ggccgccgcc gtcggaaggt
     6421 aatcgcacct ttttgtgaga ttgctactcg cgacctttgt cgcgattgca taaaaattaa
     6481 cataggagtt agttcctaca ttaggaggta ttcaatcatg acaggttcaa tcaatgctct
     6541 tgcagtggcg ctggcaatcg gcctgggagc catcggtccg ggtatcggta ttggtatcgc
     6601 ggctggccag gcgttcaacg ccattggtcg caatcctgaa gcagagccgt tggtccgtat
     6661 caacatgttc atcggtctgg cattcgctga agctatctct atttatgcgc tggttatctc
     6721 cttcgtactg agtggatcag tcaaatagga tttgcctgta ctgcggtgcg agtgcgggct
     6781 atcgataaga cgtatcgcct gtcctcgcct tcagtgacga aaggaggcgc agagaatgca
     6841 catccaggtt tttgctgatc tactcggtag tctcggcatc aatttacttg ccttcctctc
     6901 gcagctcatc agcttcggta tcgtattcct catcctgtgg cgctgggcgc taccggtcat
     6961 tctcaggacg cttgagaagc gacagagcgt cattcgggaa ggcatcgaga atgcagagcg
     7021 cgcgaggcgt gatttgcagg aggccaatgt tcgcgcggaa cagatcttgc tcgacgcgcg
     7081 acgccaggcg caggagacga ttgccctggc tgccaaagct gccgagcgcg aagcgaaccg
     7141 catccaggag gaagcgcagg tgcgagcgca ccaggtagag cagcagcaga ttgcgcgcat
     7201 ccgccaggaa gcggcgcagg cgcgcgagga actgagccgt ctcgtagtca atctgtcgat
     7261 caacgctgcc gggaaagtca ttagtaaatc ggttgacacg aacgataacc gccgcctggt
     7321 cgaagagttc gtaaccgcca gccagacaaa ggaatcgtaa tgctaaaggg agcgatcgga
     7381 cgccgctatg ccggggcgat cttcgatatt gcgatgaagc agaacacgat tgaccgcacg
     7441 cttgatgacg tgcgggagtt tgcgcgcctg ttttcccacc gaacactggc ttatctgctg
     7501 aacgagccga agattcccac gcagcgcaaa gagaccgcca tacgccaagc gctggcatca
     7561 aaggtactgc ccacgtcgct caaccttgcc ctgctggtcg tccagcgcga actggtagag
     7621 gtgatgcccg ctatcgccag ggaactcgaa caactggttc tgaattataa gaaccaggca
     7681 tatgcggaag tgacgacggc gatgccgatg gatcagcagc agcaaagcga ggtgcggcag
     7741 gcattggagc agcagacggg caaaacgatt ctgatgcaga caaaggtttc gcccgatatc
     7801 ctgg
//
LOCUS       DASZAK010000191         3175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_181606, whole genome shotgun sequence.
ACCESSION   DASZAK010000191 DASZAK010000000
VERSION     DASZAK010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181606"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..563
                     /locus_tag="VGT44_03260"
     CDS             126..563
                     /locus_tag="VGT44_03260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV2579849.1"
                     /translation="MKLTMKGDYGLRAMLDLAAYYGQGPIESADIASRQYIPEQYLDQ
                     ILMSLRKEGLVKSVRGPKGGHLLARSPAHITMAQVMQAIEGYVPPMECLPNPGFCQLS
                     PGCALREVWQKVDEMTQQILMTTTIEELARRHRTGSVETMYYI"
     gene            620..1054
                     /locus_tag="VGT44_03265"
     CDS             620..1054
                     /locus_tag="VGT44_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2579850.1"
                     /translation="MVEQSVPGKVIAIVEDDSTLAGWFRDALEYEGHWQLRFFSDGQL
                     AMRELPELCADLILLDVGLPNLDGASLYKILRGHSNTRSTPIIVITGSRDWELHRMGL
                     SAGLFLRKPFRRKELLVMIQALLGDEREGQPDDLSPGPIHDA"
     gene            1093..2028
                     /gene="cysK"
                     /locus_tag="VGT44_03270"
     CDS             1093..2028
                     /gene="cysK"
                     /locus_tag="VGT44_03270"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV2579851.1"
                     /translation="MSAHWTKDASDLVGRTPLVELRRVEPDLPAILLAKLEMFSPGGS
                     LKDRVALQMICEAETQGLLRPGGIITEPTSGNMGISLAWLAAARGYQCILTMPDTINL
                     ERRLLLRRFGAQVILTPGSQGMRGAINKVIELRTTLPNAWFPQQFYNQANPRSHRDTT
                     AVEIWEDTGGAVDMVVIGVGTGGTLTGVAEGLRARNPSVEVVAVEPATCAVLSGGRPG
                     PHRIEGLGPGFQPDTLRVDLIDRVIPVTDADAVATAISLLQREGLSVGISSGAVAWAA
                     LSEARKEEHRGKVIVTIFPSAAERYMQTILFDDLR"
     gene            2101..2928
                     /locus_tag="VGT44_03275"
     CDS             2101..2928
                     /locus_tag="VGT44_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579852.1"
                     /translation="MRQPAGDTYIFDVSRKQYLRITLLAWLLIAASLTVAVVTAVLGV
                     LFWPTYVHTFTFYLKWQDALLATLWYCSFILVSGSILTARFLFALRAGQREGVFLLRK
                     HDIALRDLSPKNLASIYWLVGTALSCFLAALVGLGPAMLIGWTLHLASPILAIGCTIA
                     AVVLSLAGLIVTLVSGSFILIGIAGSSSFSRNMGALHTYALTSQATIRVDGLALTAIY
                     PDQPETLFDLDLLEPEDRQRLLHLLRKRWLGAERPWTFRAGDEFESVLEEARLAAMG"
     gene            2996..>3175
                     /locus_tag="VGT44_03280"
     CDS             2996..>3175
                     /locus_tag="VGT44_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579853.1"
                     /translation="MPTYQAQYSYNDANQLTQTTTSTIPAGQGYTTYTLYNATNGWLT
                     GIGKTSGTTNLATYSY"
BASE COUNT          636 a         1070 c          847 g          622 t
ORIGIN      
        1 tggcgacgct tgccgtcgcc atgtgatttc ctacaccttt gcttgccagg ccattgacag
       61 caattcgcaa ctgatatatg ctagcaacaa ctatatacgg cggatatcag aggagaatcc
      121 tgctcatgaa actcaccatg aaaggcgatt atggtctgcg cgccatgctg gacctggcgg
      181 cttattatgg gcaggggccg attgaaagcg cggatatcgc cagccggcaa tacattccag
      241 agcagtatct cgaccagatc ctcatgtcac tacgcaaaga gggattggtg aaaagcgtgc
      301 gcgggccgaa ggggggccac ctgctcgcca gatcgcccgc ccacatcacg atggcccagg
      361 tcatgcaggc tatcgagggc tacgtccccc cgatggaatg cctgcctaac cctggcttct
      421 gccagctttc acccggctgc gccctacgcg aggtctggca aaaggtcgat gaaatgacgc
      481 agcaaatcct catgactacc accatcgaag agcttgcccg ccgtcaccgc accggcagcg
      541 ttgagactat gtattatata tagtgctatg gaaccatgac ctactgcggt tggaaacata
      601 caccagggag atgtagcaag tggtcgaaca atctgtccca ggcaaagtaa tcgcaatcgt
      661 cgaagacgac tccaccctcg cgggatggtt tcgcgacgcc ctcgaatacg aagggcattg
      721 gcagctgcgc ttcttctccg atggccaact tgccatgcgc gaactgcctg agctgtgcgc
      781 agacctcatc ttgctcgatg tcggcttgcc caacctcgat ggcgcttctc tctacaagat
      841 tttgcgcggc cacagcaaca cacgctccac ccctatcatc gtcattaccg gcagccgcga
      901 ttgggaactg caccgcatgg gactgagcgc cggtcttttc ctgcgcaaac ccttccgccg
      961 caaagaactg ctcgtcatga tccaggcgct gctcggcgac gaacgcgagg ggcaacccga
     1021 cgatctctct cccggtccaa tacatgatgc ctgaaattat aggcatgaat taacgaagat
     1081 taggagagca gcatgtccgc tcactggacc aaagacgcaa gcgatctcgt cggtcgcaca
     1141 ccccttgtag aactgcgccg cgtcgagcca gacctgcccg ccatactgct cgccaagctc
     1201 gaaatgttca gccccggcgg cagcctcaaa gatcgcgtcg cgctccagat gatctgtgaa
     1261 gctgaaaccc agggcctgct gcgccccggc ggcatcatca ccgagccgac cagcggcaac
     1321 atgggcatct ccctggcctg gctcgcggct gcgcgcggct accagtgcat tctcacgatg
     1381 cccgacacca tcaatctcga acgccgcctg ctgctgcggc gcttcggcgc gcaggtcatt
     1441 ctcacacctg gatcgcaggg aatgcgtggc gcaatcaaca aagtcatcga gctgcgcacc
     1501 actctcccca acgcctggtt cccccaacaa ttctacaacc aggccaatcc gcgttcgcac
     1561 cgcgacacca ccgccgtcga aatctgggaa gataccggtg gagcggtcga tatggtcgtc
     1621 ataggagtcg gcaccggcgg caccctcacc ggcgtggccg agggcctgcg cgcccgcaac
     1681 ccctcggtcg aggtcgtcgc cgtcgaaccg gccacctgcg ctgttctctc cggcggtcga
     1741 cccggccccc atcgcatcga aggactcgga cccggcttcc agcccgatac cctgcgcgtc
     1801 gatctgatcg accgcgtcat ccccgtcacc gacgccgatg ccgtcgccac cgccatcagc
     1861 ctgctccagc gcgagggtct ctccgtcggc atcagcagcg gggctgtcgc ctgggccgct
     1921 ctgagcgaag cgcgcaaaga agagcatcgc ggcaaagtca tcgtcaccat tttccccagc
     1981 gccgccgaac gctacatgca gaccatcctc ttcgacgact tgcgctaaca gcattgtaac
     2041 cacacctctt cccgattcgt ttgatagaga gcaagcaatt gagcaacgtt aagaggagct
     2101 atgagacaac ctgctgggga cacgtatatt tttgatgtca gtcgaaaaca atatttgcgc
     2161 atcaccttac tcgcctggct gttgattgcc gcatcgctca ccgttgccgt cgtcactgcc
     2221 gtgctgggcg tcctcttctg gccgacgtac gtccacactt tcacgttcta tctcaaatgg
     2281 caggatgccc tgctcgccac cctctggtat tgttcgttta tcctggtgag cggcagtatc
     2341 ctcaccgcgc gcttcctctt cgccctgcgc gccggacagc gggagggcgt gttcttgctg
     2401 cggaagcacg acatcgccct gcgcgatctc tcgcccaaga atcttgccag catctactgg
     2461 ctggtcggca cggccctgtc ctgtttcctg gccgcgctgg tcggactcgg tccggctatg
     2521 ctgatcggct ggacgcttca cctcgcgtct cccatcctgg ctattggttg caccattgct
     2581 gccgtcgtct tgagcctcgc cgggctgatc gttacgctcg tttccggctc cttcatcctc
     2641 atcggcatcg caggttcctc ttcctttagc cgcaacatgg gcgcgctgca cacctacgcg
     2701 ctgacttcgc aggcgacaat ccgcgtcgac ggcctcgcgc tgacggcgat ttatcccgat
     2761 cagcccgaaa cgctcttcga cctcgacctg ctcgagccag aagatcgcca gcgcctgctg
     2821 cacctgctgc gcaaacgctg gctgggagca gaacgcccct ggacgttccg cgcaggcgac
     2881 gagtttgaga gtgtgctgga ggaggctcgc ctggcggcga tggggtgagc tgaggatttg
     2941 atgcattccc ctgcagatcg ggagtctccc cagcgcctgg ggtgttacaa ccgcattgcc
     3001 cacctaccag gcgcaataca gctacaacga cgccaaccag ttgacccaga ccaccaccag
     3061 caccatccca gcagggcagg gctacaccac ctacaccctg tataacgcca ccaacggctg
     3121 gctgaccggc atcggcaaga cctctggaac caccaacctg gcaacgtaca gctac
//
LOCUS       DASZAK010000192         2626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_288330, whole genome shotgun sequence.
ACCESSION   DASZAK010000192 DASZAK010000000
VERSION     DASZAK010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288330"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..2272)
                     /locus_tag="VGT44_03285"
     CDS             complement(83..2272)
                     /locus_tag="VGT44_03285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2579854.1"
                     /translation="MSTIESTLPPETLPRSSRWHTIRHTLRGWLTTWDIYPILLVSCF
                     LHYYQLGITEFDVDQAVLFGMARNAVLHGLLPATSNLGSLNTAHLPAAVFLFMLPAAI
                     SSNPLGGAILVATLNVLADLLTYVFVRRYFGRVAGIVAALLFATAAAPLGYSRFIWQP
                     TMLAPFTVLFFFALFWGVVERRNGWLLPALLLLGILAQLHEEMALLAIPLLLAVLFAP
                     ETIRRRDVVYGLLALLVLFSTYIAWMIATHFHDVTVLLSVSKKPAYFDPLLFTYYRDF
                     FSPYNALAIPSDPHTLVYKLLPILLWLRRFMLILVIGGFVTALVRILLGSPLFAQIIS
                     RKGVMMSETREGQAPTASKASPWLRVWQWWRAILPSPLACGLILLLVWQVVFVLALAR
                     HSRTVPIVFHYLLALMPGSFMLVGVFIGSLAGWLRTQAQWWRHTHFALYLFMSLLLVA
                     QFAGSTAGILDQVSQQNQVVANHHSLSSLQRAFNTADLLARQQHLSHVYVSSDFYTQS
                     ALEYLAALMQTPTTVFDGARCIVLPNPADGPAILLVGPNDALTPALFAHYSSVQALPG
                     QDVAPFHLYIVRSAQSTFAFGADQTFTHELHSLDGQLQRINAGNGEFLTAWWSILKSE
                     PAAYGTTYHYGFVASISKGSGLPAQNIGSQCTLSAIRTGDEMVVAFPIAVNTIIPALV
                     SVAAQFHIEQPVPLTLGPLHLETFSTAWVSHYALRTTRGGSSVTLQA"
     gene            complement(2285..>2626)
                     /locus_tag="VGT44_03290"
     CDS             complement(2285..>2626)
                     /locus_tag="VGT44_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV2579855.1"
                     /translation="SAIKIGNPVSFTRARRVIERTQGVVEEVSDEEILAAKTVIDRAG
                     IGCEPASAAALAGIRKLVRSGIIRRDERVVGVLTGTLLKDSYTGIPQQVQSQAVEPTV
                     EAVRRVLEVLS"
BASE COUNT          616 a          737 c          810 g          463 t
ORIGIN      
        1 gctcttcccg aaaggctcga taaatgattt tcttaagacc aatgcccttg cggttgccat
       61 gctgcacacc gcgtttcaga tatcaagctt gcagagtcac gcttgaccca ccacgtgtgg
      121 ttcgcaaggc ataatgactt acccacgcgg tggaaaacgt ctcaagatgg agcggcccca
      181 gtgtcagagg aactggctgc tcaatatgga actgggccgc gacgctcacc agggcgggta
      241 tgatagtatt gacggctatg gggaaggcaa cgaccatttc gtcgcctgtc ctgatcgcgc
      301 taagggtaca ctgactgcct atattctgtg ctggcaaccc gctgcccttg ctgatcgacg
      361 ccacaaaccc ataatggtag gttgtaccat acgcagcagg ttcgcttttc aaaatactcc
      421 accaggccgt caaaaattcg ccatttccgg cattgatgcg ctgaagctgc ccatcgagcg
      481 aatgcagttc atgcgtgaag gtctgatcgg ccccgaatgc gaaagtcgat tgcgctgatc
      541 gcacaatgta caggtggaat ggcgcgacat cctggccggg tagcgcttgc acggagctat
      601 agtgcgcgaa cagcgccgga gtgagcgcat cgttcggccc taccagcaga atggctgggc
      661 catcggcggg attgggcagc acgatgcagc gcgccccatc aaacaccgtc gttggcgtct
      721 gcatcagcgc cgccaggtat tctaaagcag attgggtata aaagtcagac gagacataga
      781 cgtgggagag atgctgctgt cgcgccagga gatcagccgt gttgaaggcg cgttgcagcg
      841 aactcaggct gtgatggttg gctacgacct ggttctgctg gctcacctgg tcgagtattc
      901 cggcggttga cccggcgaat tgcgcgacca gcaggagact cataaagaga tagagcgcga
      961 agtgagtatg tcgccaccac tgcgcctgtg tccgcagcca gccggccaga gagccgataa
     1021 agactccaac cagcataaac gatccaggca tcagcgcgag caggtagtga aagacgatgg
     1081 gaacggttct ggaatgcctc gccagcgcca gcacgaagac cacctgccag actagcagca
     1141 ggatcagccc gcaggccaat ggcgacggca agatcgcacg ccaccattgc cagacacgca
     1201 accagggaga agcttttgac gcggtgggcg cttgcccctc gcgcgtttcg ctcatcatca
     1261 cacctttcct ggagataatc tgcgcaaaca aaggggaacc gagcaaaatc ctgacaagcg
     1321 cggtcacaaa gccgccgatc accagaatca gcataaagcg gcgcagccac agcagtatgg
     1381 gcaaaagttt gtagaccagc gtgtgcggat cggaggggat ggccagcgcg ttataagggc
     1441 taaagaaatc gcgataatac gtgaacagga gtggatcgaa ataagcaggt ttcttcgaga
     1501 cgctcaacag aacggtcaca tcgtggaagt gcgtggcgat catccaggcg atatacgtag
     1561 agaagaggac gagcagtgcc agcaggccgt atacaacatc gcgcctgcgt atcgtctcag
     1621 gcgcaaataa cacagccagc agcagcggga tggccagcag cgccatctct tcgtgcagtt
     1681 gagccaggat gccgagcagc agcagggcag ggaggagcca gccgttgcgc cgctcaacca
     1741 cgccccagaa cagggcaaag aaaaacagca ccgtgaaggg cgccagcatg gtcggctgcc
     1801 agatgaagcg gctgtagccc agcggcgcgg cggcggtcgc gaaaagcagc gctgcgacga
     1861 tacccgccac gcggccaaag tagcgccgca cgaacacata cgtcagcagg tctgccagga
     1921 cattgagcgt cgcgacgagg atcgcgccgc ccaggggatt gctactgatg gcggcaggca
     1981 gcataaacag gaagacggcg gcaggcagat gggctgtatt gagcgagccg aggttgctcg
     2041 tagccggcag cagtccatgc aggacggcat tgcgagccat gccgaagagc acagcctggt
     2101 caacgtcaaa ttcggtgatg ccgagctggt aataatgtaa gaagcaggag acgagtagga
     2161 tcggatagat gtcccaggtc gtcaaccagc cgcgcagggt gtggcgtatc gtatgccaac
     2221 gcgatgagcg tggcagtgtt tctggtggca gtgtcgattc gatggttgac atatcgcgac
     2281 tcacctacga aagaacctcc aatacccggc gtaccgcttc gacagttggc tcgaccgcct
     2341 ggctttgcac ctgttgcggg atgccggtgt agctatcctt gagcagcgtt cccgtcagaa
     2401 cgccgacgac gcgctcatcg cgccggataa tgccgctccg gacgagcttg cgaataccgg
     2461 cgagcgcggc ggcggaggcc ggttcgcagc cgatgcccgc gcggtcgatg acggttttgg
     2521 ctgccagaat ctcctcgtcg ctgacctctt ccacgacgcc ctgtgtgcgt tcgatcacgc
     2581 ggcgtgcgcg cgtgaagctg accgggttgc cgatcttgat agcgct
//
LOCUS       DASZAK010000193         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_5082, whole genome shotgun sequence.
ACCESSION   DASZAK010000193 DASZAK010000000
VERSION     DASZAK010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5082"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VGT44_03295"
     CDS             <1..828
                     /locus_tag="VGT44_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579856.1"
                     /translation="HIGFVTALFTDGLILGETLGAGATVPLMLWLFGNNAWRATFVLW
                     GLPVVVLLAFWLWLAPREPQVEDVEPAVNAQVSERTTMPEPTSTSVAAQAPVRTIHLG
                     LLLGAGSLIYFGMNNWIATYNQALHRDSFTPLALTVLNALQLPSSLGVTFFAQRLVGK
                     RWPFVLAGIVCIVAMLTWIFGPASLEYPCAGLLGASSALVFTLGLALPALLANPTNVA
                     RLAGATLSIGYSTAFIGPFIGGGLWDLFGLPALAFVPVVVASASLVVAGLMLPARHA"
     gene            988..2007
                     /gene="gnd"
                     /locus_tag="VGT44_03300"
     CDS             988..2007
                     /gene="gnd"
                     /locus_tag="VGT44_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEV2579857.1"
                     /translation="MQLGLVGLGRMGANMAKRLLRNGHTVVGMARRDTTVQAALDEGS
                     ITSGTTSLAELVKRLDTPRAVWLMVPAASVDDTIADLVPLLSPGDTIIDGGNSYYHDD
                     IRRAAELKPKSIHYLDIGTSGGVWGLDRGYCLMIGGETEVVQRLSPIFETLAPGVGAT
                     SRTPGREQVGGTAEKGYLHCGPTGAGHFVKMVHNGIEYGIMAAYAEGLNILHNANVGK
                     HQQSVDAETSPLAHPEYYQYDLNLADVSELWRRGSVIGSWLLDLTASALLKDPQMQSF
                     GGRVSDSGEGRWTISAAIDEGVPAHVLSAALYERFASRGNADFASQLMSAMRFEFGGH
                     LEKKE"
     gene            2098..3732
                     /gene="pgi"
                     /locus_tag="VGT44_03305"
     CDS             2098..3732
                     /gene="pgi"
                     /locus_tag="VGT44_03305"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV2579858.1"
                     /translation="MSVQATNPIPLTQRPEWKALEEHFVAIRGVHLRELFARDGQRGE
                     RLALEAAGLYLDYSKNRVTDETLRLLLRLAESAGLRQRIDAMFSGEKINITEQRAVLH
                     VALRAPKGESILVDGEDVVPEVHKVLEKMAGFANRVRSGAWTGYTGKRIRNIVNIGIG
                     GSDLGPFMAYEALKFFSDRNLTARFVSNVDSNEFVEATRDLDPAETLFVVSSKTFTTQ
                     ETLTNARSARQWCVSALGSEQAVAKHFVAVSTNAQEVAKFGIDTANMFEFWDWVGGRY
                     SYDSAIGLSLMIAIGPDHFYEMLSGFHAMDEHFRTAPFERNLPVLLGLIGIWYNNFFG
                     AETVAILPYDHYLGRLPSYLQQLDMESDGKSVDLEGWRVNYQTGPIIWGQPGTNGQHA
                     YYQLIHQGTKLIPCDFIGFSKSLNPLGPHHDLLMANFFAQTEALAFGKTAEEVTADGV
                     PAFQVPHRTFEGNHPTNTILAERLTPETLGRLIALYEHKVFVQGTIWNINSFDQWGVE
                     LGKALASRIVPELTSVAELKHDSSTNALIRRYRRER"
     gene            complement(3742..>3987)
                     /locus_tag="VGT44_03310"
     CDS             complement(3742..>3987)
                     /locus_tag="VGT44_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HEV2579859.1"
                     /translation="VEGESTAQPQAQPPRGREQAYTVWHEITLHPGQQYTILPDTLHW
                     FQAGDSGAIVSEFSTRSRDEYDVFTDPAIRRETVVNS"
BASE COUNT          751 a         1128 c         1305 g          803 t
ORIGIN      
        1 cacatcggtt ttgtgacggc gctttttacc gatggcctga tcctgggcga gacgctgggc
       61 gcgggcgcga cggtgccgtt gatgctctgg ctgtttggga acaatgcctg gcgcgctacg
      121 ttcgtgttgt gggggctgcc ggtggtagtc ttgctggcgt tctggctgtg gcttgccccg
      181 cgcgaaccgc aggtggaaga tgtggaacca gcagtgaatg cccaggtgag cgaacgaact
      241 acaatgccgg agccgacttc aacatctgtt gccgcgcaag cgccggtcag gacgatccac
      301 ctgggcttgc tgttgggcgc gggcagtctg atctactttg gcatgaataa ctggatcgcc
      361 acctataacc aggcgctgca tcgagacagc ttcacgccgc tggccctaac ggtccttaat
      421 gccctgcagc tgccgtccag cctgggcgtg accttcttcg cgcagcggct ggtgggcaaa
      481 cgctggccgt ttgtgctggc gggtattgtc tgtatcgtcg ccatgctcac ctggattttc
      541 ggcccggcca gcctggaata tccctgcgcg ggcttgctcg gcgcgagttc cgcgctggtc
      601 ttcacgctgg gtctggcgct ccccgcgctg ctggcaaacc cgacgaatgt ggcgcgactg
      661 gcgggcgcaa cgctttcgat cggctacagc accgccttca tcggtccatt catcggcggt
      721 ggtctctggg acctgttcgg cctgcccgcg ctggcctttg tgccggtggt ggtcgccagc
      781 gcgtcgctgg tcgtcgcggg gctgatgctg cctgcaagac atgcatagtc gggtggctca
      841 ttccccattt gacagccctc gctgtacgtg cataatgaaa gcagtgtaac gtgcgacatg
      901 attgggaaca ctaatcggtg aaaagcgagt tatacgtgat atttttaagc gacacattgg
      961 tacaaaagca aggaaaggga tagaacgatg caacttggac tggttggact tgggcgcatg
     1021 ggcgcgaata tggcgaagcg gctgctgcgc aacgggcata cggtggttgg catggcgcgc
     1081 cgcgacacga cggtgcaggc ggccctggac gaaggctcga ttacgagcgg gacgacctcg
     1141 ctggcggaac tcgtcaagcg acttgacacg cccagggcgg tctggctgat ggtgcctgcc
     1201 gcctcggtgg atgacactat cgctgatctg gtgccgctgc tttcgccggg agatacgatt
     1261 atcgacggcg gcaactcgta ttatcacgac gacatacggc gcgctgccga gttgaagccg
     1321 aagagcatcc actacctgga tatcggcacg agcggcggcg tgtggggcct ggatcgcggc
     1381 tactgcctga tgattggcgg cgagaccgag gtggtgcagc gcctctcgcc gatcttcgag
     1441 acgctggctc ctggcgtggg ggcgacatcg cgcactcccg gtcgcgagca ggtgggcggc
     1501 acggcggaaa agggctattt gcactgcggg ccgaccggcg cgggtcactt cgtcaagatg
     1561 gtgcataacg gcattgaata tggcattatg gccgcctatg ccgagggcct caatatcctg
     1621 cacaacgcca atgttggcaa gcaccagcaa tcggttgacg ctgaaacctc gcccctggcg
     1681 catccagaat actaccagta cgatctcaac ctggctgatg tctcggaact gtggcggcgc
     1741 ggcagtgtga tcggctcgtg gctgctcgat ttgacggcca gcgcgctgct gaaagacccg
     1801 cagatgcaga gtttcggggg ccgcgtttcc gactctggcg aggggcgctg gacgatctcg
     1861 gcggccatcg acgagggagt gcccgcgcat gtcctcagcg cggcgctgta cgaacggttc
     1921 gcctcgcgcg gcaacgccga tttcgccagc caactcatgt cagccatgcg cttcgagttc
     1981 ggcggccatt tggaaaagaa agaataacca aatggggcat ggtatgaacc gttagtaggt
     2041 aatcatcggc tcctacggtg ttccgtaccc ctcctacaga taaggagtaa tatcattatg
     2101 tcggtacaag caacaaatcc aataccattg acgcagcgac cggaatggaa ggcgctggag
     2161 gagcattttg tggcgatacg tggggttcac ctgcgggagt tgttcgcgcg ggatggacag
     2221 cgcggggaac ggctggcgct tgaggcagcc ggattatacc tggattattc gaagaaccgc
     2281 gtgacggatg agacgctgcg cctactgctg cgcctggcgg agtcggcggg tctgcgacag
     2341 cgcattgatg cgatgttcag tggggagaag atcaatatta cggagcagcg cgccgtgctg
     2401 cacgtggcgc tgcgcgcgcc gaaaggggag tcgattttag ttgatggcga ggatgttgtg
     2461 ccggaagtcc acaaggtgct ggaaaagatg gccggtttcg ctaatcgcgt gcgcagcggc
     2521 gcgtggacgg gctatacggg caaacgcatt cgcaacatcg tcaatatcgg cataggaggc
     2581 tctgacctgg ggccgttcat ggcatacgag gcgctgaagt tttttagcga ccgcaacctg
     2641 acggcgcgct tcgtctccaa cgtggatagc aacgaatttg tggaggccac gcgcgacctc
     2701 gacccggctg aaacgctctt tgtcgtctct tccaagacct tcacgacgca ggagacgctc
     2761 accaacgcgc gctctgctcg ccagtggtgt gtgagcgcgc tgggcagcga gcaggcggtg
     2821 gcgaagcact ttgtggccgt ctcgaccaac gcgcaggagg tggcgaaatt tggcatcgat
     2881 accgccaata tgttcgagtt ctgggactgg gtcggtggcc gctattccta cgattccgcc
     2941 atcgggctat cgctgatgat tgcgattggt cccgaccact tctacgagat gctgtccggt
     3001 ttccacgcca tggacgagca ttttcgcacc gcgccgttcg agcgcaatct gccggtgctg
     3061 ctggggctga tcggcatctg gtacaacaac tttttcgggg ctgaaactgt cgccatcctg
     3121 ccctatgacc actacctggg gcggctgccc agctacttgc agcagctcga catggaaagc
     3181 gacggcaaaa gcgttgacct ggaagggtgg cgtgtgaact accagacggg accgatcatc
     3241 tgggggcagc cgggaacgaa tggccagcac gcctactacc agctcatcca ccagggaacg
     3301 aagctgatcc cgtgcgactt catcggcttc agcaaatcgc tcaacccgct tggaccgcat
     3361 cacgacctgc tgatggccaa cttcttcgcg cagaccgagg cgctggcctt cggtaagacg
     3421 gcggaggagg tcacggcgga tggtgtgcca gccttccagg tgccgcatcg caccttcgag
     3481 ggcaaccacc cgaccaacac catcctggcg gaacgcctga cgcccgaaac gcttggccgg
     3541 ctgatcgcgc tctacgaaca caaggtcttc gtgcagggta ccatctggaa catcaactcg
     3601 ttcgatcagt ggggcgtcga actgggcaag gcgctggcca gccgcatcgt ccccgaactg
     3661 acaagcgttg cggaactgaa gcacgacagc tcgacgaacg cgctgatcag gcggtacagg
     3721 cgggagcgat aggcgtggta atcaagaatt taccacggtt tcgcggcgga tggcgggatc
     3781 ggtgaagacg tcgtattcgt cgcggctgcg cgtggagaat tctgagacaa tcgcgccgct
     3841 gtcgccagcc tggaaccagt gtaacgtgtc gggcaggatg gtgtattgct gacctggatg
     3901 gagcgtgatt tcgtgccaga cggtgtaggc ctgctcgcgt cctcgtggcg gctgcgcttg
     3961 tggctgcgcg gttgactcgc cttccac
//
LOCUS       DASZAK010000194         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_181684, whole genome shotgun sequence.
ACCESSION   DASZAK010000194 DASZAK010000000
VERSION     DASZAK010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181684"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VGT44_03315"
     CDS             complement(<1..768)
                     /locus_tag="VGT44_03315"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV2579860.1"
                     /translation="MFRLEKKIAFVTGAGSGIGESIARLFAQQGAHVVLADISMDAAE
                     RVAAEIRSAGGNTRAQQLDVADEARVRAAIERVAATEGRLDILVNNAGISHVGNILET
                     SMEDWERVMRVNAGGVFLCAREGVRQMLAQSPVGGSIINMSSVVAMIGVDRRLPYSAS
                     KGAVLALTRSIAIDFVAQGIRCNAICPGTVHTPFVEGYLARNFPGREDEVRQQLHARQ
                     PLGRMGRPDEIASAALYLASDEAAFVTGSALVIDGGWT"
     gene            complement(788..1111)
                     /locus_tag="VGT44_03320"
     CDS             complement(788..1111)
                     /locus_tag="VGT44_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEV2579861.1"
                     /translation="MRRVGQVIGLKADQVVAYERLHAAVWPEVLAMIAACNIHNYSIF
                     RHGELLFAYFEYTGDDYEADMAKMAADPKTREWWTLTDPMQTPLETRPPGAWWTPIKE
                     VFHTD"
     gene            complement(1114..>2425)
                     /locus_tag="VGT44_03325"
     CDS             complement(1114..>2425)
                     /locus_tag="VGT44_03325"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HEV2579862.1"
                     /translation="VALTIVGVQAYDIRFPTSRTLAGSDAMNNDPDYSAAYVVLQTDH
                     PGGLEGHGLTFTIGRGNEVCVAAIRALAPLVVGRTLESFTGDRRAFWRYITGDSQLRW
                     IGPDKGAMHLATGAVVNAVWDLWAKVEGKPLWKLLADMTPEQLVSCIDFRYISDALTP
                     DEALDLLRRQAPTRAQREAEMLQQGFPAYTTGAGWSGYSDERMRLLCREALANGWRHF
                     KLKVGLSLEDDLHRARIMREEIGPESTLMFDANQVWEVKEAIAAMQGLAAFDPFWIEE
                     PTSPDDILGHATIARAIAPIRVATGEHVQNRIIFKQLFQANAIAICQIDACRVGGVNE
                     VLAILLMAAKFGVPVCPHAGGVGLCEYVQHLSLFDYIVISGSLESRMIEYTPHLHEHF
                     VHPAIIKHGRYVPPDAPGYSVTMYAESIAAYSYPDGSEWAGERR"
BASE COUNT          484 a          767 c          709 g          465 t
ORIGIN      
        1 ggtccagccg ccatcgatga ccagggcgga gccggtcaca aaggccgcct cgtccgaagc
       61 caggtagagc gcggcggagg cgatctcgtc gggtcgtccc atgcgtccga ggggctggcg
      121 ggcgtgcagt tgctggcgca cctcgtcctc gcgacccgga aagttgcggg cgagatagcc
      181 ttctacgaac ggcgtatgca ccgtgccggg gcagatggcg ttgcagcgaa tcccctgcgc
      241 aacgaaatcg atggctatcg agcgcgtcaa cgccagcacc gctcccttgc tcgcgctata
      301 gggcagacgt cgatcgacgc cgatcatagc aaccaccgaa gacatgttga tgatcgagcc
      361 tccgaccggt gactgagcca gcatctgccg caccccttcg cgcgcgcaca ggaagacgcc
      421 ccccgcattg acgcgcatca cgcgctccca gtcctccatg ctagtctcca ggatgttgcc
      481 gacgtggctg atgccagcat tgttgaccag gatatcgagc cgaccttcgg tagcggcaac
      541 gcgttctata gcggctctga cccgggcctc atcggcgaca tcgagctgct gagcgcgtgt
      601 gtttcctccg gcagaccgaa tttcagcggc gacccgttcg gcggcatcca tgctgatatc
      661 ggcgagaacg acgtgcgcgc cctgctgcgc aaagagccgc gcgatggact ctccgatgcc
      721 cgacccggct cccgtgacga aggcgatttt cttctctaga cgaaacattt ctgcttcctt
      781 tcttccgcta atcggtatgg aacacttcct tgatcggggt ccaccatgcg ccgggcgggc
      841 gtgtctccag cggcgtttgc atgggatcgg tcaaggtcca ccattcgcgc gtctttggat
      901 cggcagccat cttcgccata tcggcttcat aatcgtcgcc ggtatattca aagtacgcaa
      961 agagcagctc gccatgacga aatatgctgt agttgtggat gttgcacgcg gcaatcatcg
     1021 ccagcacttc tggccagacg gccgcatgga gacgttcgta ggctacgacc tggtcagctt
     1081 tcaggccgat gacctgtcca actctgcgca tgattacctc ctttcgccag cccactccga
     1141 cccatcggga taagagtagg cggctatcga ctcggcatac atcgtcacgc tgtatcctgg
     1201 agcgtccggg gggacatagc ggccatgctt gatgatggcc ggatgcacaa aatgctcgtg
     1261 caagtggggg gtgtactcga tcatgcggct ctccagcgag ccgctaatca caatatagtc
     1321 gaagagcgag agatgctgca catattcgca caggccaacg ccgcccgcgt gtgggcagac
     1381 cgggacgccg aacttagcag ccatcaggag aatcgccagc acctcgttta ccccaccgac
     1441 gcggcaggca tcgatctggc aaatggcgat ggcgttcgcc tgaaacagtt gtttgaagat
     1501 gatgcgattt tgcacatgct cgccggtggc aacgcgaatg ggcgcgatgg cacgcgcaat
     1561 cgtggcatgt ccaagaatat cgtcagggct ggtgggttct tcgatccaga aagggtcaaa
     1621 tgccgcgagg ccctgcatgg ctgcgatagc ctctttcact tcccacacct ggttcgcatc
     1681 gaacatcaag gtactctcgg gtccgatttc ttcgcgcatg atacgcgcgc gatgcagatc
     1741 gtcctccagg ctcaggccaa ccttcagttt gaagtgccgc cagccgttcg ccagcgcttc
     1801 gcggcaaagc aggcgcatcc gctcgtcact atagcccgac caacccgcac cggtggtata
     1861 ggcaggaaaa ccctgctgga gcatttccgc ctcgcgttgc gccctggttg gcgcctggcg
     1921 acgtagcaag tcgagcgctt catctggagt cagggcatcg ctgatatacc ggaaatcgat
     1981 gcaggacaca agctgctccg gggtcatatc ggcgagcagc ttccagagcg gctttccctc
     2041 caccttggcc cacaggtccc aaaccgcgtt gacaaccgcc ccggtggcca ggtgcatcgc
     2101 tcccttatcc ggcccgatcc agcgcaactg actgtcgccg gtgatatacc gccagaaggc
     2161 gcgcctgtcc ccggtgaacg actccagggt gcgcccgacg acgagcgggg ccagcgcccg
     2221 gatggctgcg acgcacacct cgtttccacg cccgatagta aaggtcaaac catgcccttc
     2281 cagtccaccc ggatggtcgg tctgcaatac cacgtaggcc gcggaataat ccgggtcgtt
     2341 attcatggca tcagaaccgg ccagggtccg tgatgtaggg aaacgaatat cgtaagcctg
     2401 gacaccaaca atagtgagcg ccaca
//
LOCUS       DASZAK010000195         4911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_253740, whole genome shotgun sequence.
ACCESSION   DASZAK010000195 DASZAK010000000
VERSION     DASZAK010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253740"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..2799)
                     /locus_tag="VGT44_03330"
     CDS             complement(34..2799)
                     /locus_tag="VGT44_03330"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2579863.1"
                     /translation="MLHFMRRSLLVQLLSVYLLFVVVVLLGGVAVNVLVEQQLSNDAQ
                     ASDQALAQEIALQTGLRLHSAEDALVAFSKLIVLPRTTAAVESSIQAFESARSDVDQV
                     YWLDPVGGLRVSCSRAACESKLVAEFSPPDVVRRARVATGPVFEVGVAVEPTFNAGVI
                     IAEPVYVASGQLAGIVAASLSLKELSVPLTNVVQAQQHQGRRLMISIIDDQGRLIATP
                     QPERILWTVLNELPGADQALQGHTASRMGPGPDGQNWLFSAVPVSNAGWAVVVQRPES
                     EALAVVTQFHLWLLIAALLFAIGGLLFWLMLLMRVIRPLHTLAIQHQSLPTSEQSIPM
                     DAATLAARDDEVGDLARSLVRLERDGLKKLGELRTLLETSNAVVGSLEPHAVVGKIIR
                     EVRRLVDVQAAAVLLPDDEGVLRVLVSDGHSERYDHKLSLPPENVSSSAVLALRDGKP
                     VQKLLSSDQSVPSLSYDEGFRSVLAIPIISRHAGGVVLLVHRTEPHPFNSNEIDLLLT
                     FANYATLAWEHAVLYERSDERLREVAQENERLYQQASEEKQRLEAIMGSMSDGLVLTG
                     IEGTILYANLGTSAIVGLPSESLEGRPISALYEALRGAALDSEDCERVIGQTELAEAT
                     ELVVEIKQSDRRRAIHLRLFDVCDESGRAIGRGLLLRDITRERELDEFKSALLAAVGH
                     ELRTPLAAIKGHASTLLQGDVTWPLADQRHFLKTISGEADRLAQLVNNLLDLSRQEAG
                     LLLLNRGPTRVQDLVSKTIERLSHPGFMISSHIPEDLPLVNVDSGRIEVVLRNLVANG
                     LVYGEDEVNINAARRDNVIVVSVSDNGPGIAPDELPHVFERFYRTRYSQQHYSGGTGL
                     GLTICKAFIEAHGSVIWAESSARGTTISFSLPLSVSDQVVSPVGFSIEEGGAYDDEGK
                     AHSTRRR"
     gene            complement(3023..4078)
                     /locus_tag="VGT44_03335"
     CDS             complement(3023..4078)
                     /locus_tag="VGT44_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2579864.1"
                     /translation="MNSWSRRLCLTTGIVLILILSACGSSGTTTTTTTSNGTTTTTTH
                     LSIMVGGLSKQIYLPNELAQRLGYFAKQGLDVTLIDEASGQSSEDAVLADQVNAGSGS
                     YNHTIELQAAGKNMECVVQLDVAPGEAEMVSAKEASQIQSVKDLKGKNLGVTELGSGT
                     QTLTIALLHNAGIPQDQAHFVPVGAGDTFIAAMQQGHIDAGMTTEPTISRLLATGVGK
                     VLVDLRTPQSTQAALNGPYPFICVFMKNDYVNSHKDVVQKLVNAYVMTLKYIQTHSAD
                     EIAAQMPADYYAGNKDLYVTALKNQLTIFSPDGKMPTGGPESVLGIENQTNKTVQGKQ
                     IDLNTTYTNDFASSATG"
     gene            complement(4229..>4911)
                     /locus_tag="VGT44_03340"
     CDS             complement(4229..>4911)
                     /locus_tag="VGT44_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579865.1"
                     /translation="QPSGIWNQLVTWVTDGTALGPLWEQIAVTLEEAVLGFAIGVVLG
                     VIFGVVLGRNRFLSDVLSPYIKAANSIPRIVLGPIFVIGLGLGIQSKIALAAVLVFFI
                     VFFNAFQGVREVDRNLLANARILGASPRQLSSSVIIPSAMSWIFASLHTSFSFALVGA
                     VVGEFLGSTEGIGLMIQNAGATFNANGVFAAMFILALVALITETLITSLENRLIRWRP
                     NTVSEVTI"
BASE COUNT         1075 a         1452 c         1328 g         1056 t
ORIGIN      
        1 aaattccggc tcacgaaatc ccgcaacgcc acttcatctt cggcgagtag aatgcgcttt
       61 cccttcatcg tcatacgcac ccccttcctc tatcgagaat cccaccggcg agacaacctg
      121 atcagaaacg gaaagcggca gcgagaacga aattgtggtt ccccgcgcgc tgctttccgc
      181 ccagatgacg cttccatggg cctcgatgaa ggctttgcag atggtcaatc ccaggccggt
      241 gccaccagaa tagtgttgct ggctatatcg cgtgcgatag aaacgctcga acacgtgtgg
      301 cagctcgtcg ggggcgatgc caggcccatt gtccgaaact gaaacgacaa tcacattgtc
      361 ccgccttgcg gcgttaatgt tcacttcatc ctcaccatac accagcccgt tggcgaccag
      421 gttgcggagc acgacctcaa tgcgcccact gtccacattg accagaggca ggtcttcagg
      481 aatgtgcgaa gaaatcataa accccggatg actcagtcgt tcgatggtct tcgacacgag
      541 atcctgcact ctggtaggcc cgcgattgag cagaagcaac ccggcttcct ggcgcgagag
      601 gtcgagcaag ttgtttacga gctgtgccag ccgatcggct tcgccactga tcgtcttcag
      661 gaaatggcgc tggtccgcga gtggccaggt tacgtctccc tggagcaagg tcgaagcgtg
      721 gcctttgatc gccgccaatg gcgtgcgcaa ttcgtggccg acagccgcca gtaacgccga
      781 cttgaattcg tctagctcgc gttcgcgcgt tatatcccgc agcagcaggc cgcgcccaat
      841 ggcccgacct gattcgtcgc acacgtcgaa cagccgtaag tggatggcac gacgcctgtc
      901 agattgttta atctcaacaa ccaactccgt ggcttcggcc aactcagtct gcccaataac
      961 ccgctcgcaa tcctcggaat ccaacgcagc cccgcgcagc gcttcgtaga gcgcgctgat
     1021 ggggcgtccc tcaagtgact cgctaggtaa gcctacaata gcgctcgttc ccaggttggc
     1081 atacagaatc gtcccctcga tgcctgtcag gaccagccca tcgctcatac tgcccatgat
     1141 cgcctccagc ctctgcttct cttccgaggc ttgttggtac agtcgctcgt tctcttgagc
     1201 tacctcgcgt agtcgctcgt cgctgcgctc atagagcact gcgtgttccc aggcgagtgt
     1261 ggcgtagttg gcgaacgtca acagcaagtc gatctcattc gaattgaatg gatgaggctc
     1321 ggtgcgatgc accagcagga ccacaccacc cgcgtggcgg ctgatgatgg ggatcgccag
     1381 cacagagcgg aagccctcgt cataggagag cgatggcact gattgatcgg aactgagcag
     1441 cttctgcact ggcttgccat cacgtagagc cagcaccgct gacgaactca cgttctcagg
     1501 gggcagggaa agtttgtggt cataacgttc actatggccg tcgctgacca gtacgcgtaa
     1561 tacaccctca tcgtctggca agagtactgc cgcagcctgc acatcaacca gccgtcgcac
     1621 ctcgcgaatg atcttgccta ctacagcatg cggctcaagc gatccgacaa cggcattcga
     1681 cgtttcgagc aacgtacgca gttcgccaag ttttttcagc ccgtcgcgct ccaggcgcac
     1741 aagtgagcgt gccaggtcgc caacttcgtc atcacgcgcc gcgagtgtgg ctgcatccat
     1801 tgggatcgat tgctccgagg tgggcagcga ctggtgttgg atggcgagtg tatggagggg
     1861 gcggatgacc cgcatcagca gcatgagcca gaacagcagg ccaccgatgg cgaagagcag
     1921 tgcagctatt aacagccaga gatggaactg cgtgactaca gccagagcct cgctctcggg
     1981 cctctgcacg acaacagccc agccggcatt cgatacggga accgcgctga acagccagtt
     2041 ctggccatca ggaccaggcc ccatgcgtga ggccgtgtgg ccctgcagcg cctggtctgc
     2101 tcctggtagc tcgttcagta ctgtccacaa gatgcgctca ggctggggcg tggcgatgag
     2161 tctgccctgg tcgtcgatga tgctgatcat gagtctccgg ccctggtgct gttgggcctg
     2221 aacgacattc gtcaggggga cgctgagttc tttgagcgag aggctggccg ctacaatgcc
     2281 ggccaattga ccactggcaa cgtacacagg ttcggcgatg atgacaccag cgttgaaggt
     2341 cggttcaacc gcgacaccga cttcgaagac cgggccagtg gccacacggg ccctccggac
     2401 gacatcgggt ggcgagaact cagcgactag cttcgactcg cacgcggcac gcgaacagga
     2461 gacgcgcagc ccgcccaccg ggtcgagcca atagacctga tccacgtcac tgcgagcaga
     2521 ttcgaaagcc tgaatggaac tctcaaccgc ggcagtcgta cggggtagta caataagctt
     2581 actaaacgcg acaagcgcgt cctcggcact gtgcagtcgg aggccggtct ggagcgcgat
     2641 ctcttgcgct agcgcctggt ctgatgcctg ggcgtcattg ctcagttgct gctcgaccaa
     2701 aacgttgact gccacgccac cgagcagcac taccacgaca aagagcaagt atacactcag
     2761 caactggacc aggagagatc gacgcatgaa atgcagcatg gccagtactc ctcttgccct
     2821 ggacaacata cccatagatc aaatatcacg tcccgcatct gagtatcaat tcgcgtgcat
     2881 tataccactt tggttaccca ggttgatgac tcaggctctt aacacgtata actcaaccca
     2941 ttctcgtttt catgctcacc aggcaaaacg gctcgagcgc tggcctccag gccagcactc
     3001 gagctttccc gccaggcata gattagccgg tcgcactact tgcgaagtca ttcgtgtatg
     3061 tcgtgttgag atcgatctgc ttaccctgca cggtcttatt cgtctgattc tcgatgccca
     3121 gtaccgactc agggccacca gtaggcattt tcccatcagg gctgaagatg gtcaactgat
     3181 ttttcaacgc cgtcacatac aggtctttat tgccggcgta gtagtctgct ggcatctggg
     3241 cagctatctc atccgctgaa tgcgtttgga tgtacttcaa ggtcatgacg taggcattca
     3301 ccagcttttg caccacgtct ttatggctgt tgacataatc gttcttcata aagacgcaaa
     3361 tgaaagggta aggcccattg agcgccgcct gggttgactg tggtgtgcgc agatctacca
     3421 ggacttttcc gacgcccgtc gccaggagtc gcgagattgt cggttcggtc gtcatgcccg
     3481 catcgatatg tccctgctgc attgccgcga taaacgtgtc accggcaccc accggtacaa
     3541 agtgcgcctg gtcctgtgga atcccagcat tgtgaagcag tgcgatggta agcgtttgtg
     3601 tgcctgagcc gagttcggta acacccaggt tcttgccttt aaggtctttg actgattgga
     3661 tctggctagc ctctttggca gaaaccattt cggcttcacc tggtgcgacg tccagttgga
     3721 ccacgcactc catattcttt cccgccgcct gaagttcgat ggtatggttg tatgaccctg
     3781 aaccagcatt cacctggtct gccagcactg cgtcctcgga cgattgccca gatgcctcgt
     3841 cgatcagcgt tacatcgagg ccctgcttag caaagtaccc tagccgctgg gcaagctcgt
     3901 tcggcaaata aatctgcttg ctgagaccac cgaccataat cgagagatgc gttgttgtgg
     3961 tagtggtgcc attgctggta gtagtggtgg tggtggtacc actactgcca cacgcactga
     4021 ggatgagaat taagacgatg ccagttgtga gacaaagtcg tcgactccac gagttcatat
     4081 gccttttcct tccctttctt gatggtcaca tgagagacca gataatgtcg ccaacaaatc
     4141 cgattgatcg ggcgctttcc tagccacggt gatacgtgcc gtgactttat ttctactcct
     4201 ttgtcctttc tcctttccca ggtatctcct agatagttac ttcgctgacg gtatttggcc
     4261 gccacctgat caggcgattt tccagcgagg tgataagtgt ttccgtaatc aacgctacta
     4321 acgcgaggat gaacatagct gcgaagacgc cattggcgtt gaatgtggcc ccagcattct
     4381 ggatcatcag gccaatgccc tctgtcgagc caagaaactc acctaccacc gccccaacca
     4441 gcgcaaagct aaagctggta tgcaggctgg cgaagatcca cgacattgct gatggaatga
     4501 tcacgctgct agagagttgg cgcgggctcg cccccaggat gcgcgcattc gctagcaggt
     4561 tgcggtcgac ctcgcgcact ccttgaaagg cgttgaaaaa cacgatgaag aagaccagca
     4621 cggcggcaag cgcgatcttg gattgtatgc ccagaccaag cccgatgaca aaaatgggac
     4681 cgagcacgat acgtggaatc gaattggccg ccttgatgta agggctcagt acatccgaaa
     4741 gaaagcgatt ccgtccaagc accacgccaa aaatgacgcc taaaaccacg ccgattgcga
     4801 aacccaggac agcctcttcc aacgtgacgg cgatttgctc ccaaagaggt cccagggccg
     4861 tgccatctgt tacccacgtg accagctgat tccagatacc cgaaggctgg c
//
LOCUS       DASZAK010000196         2020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_217936, whole genome shotgun sequence.
ACCESSION   DASZAK010000196 DASZAK010000000
VERSION     DASZAK010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217936"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1823)
                     /locus_tag="VGT44_03345"
     CDS             complement(<1..1823)
                     /locus_tag="VGT44_03345"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF012532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV2579866.1"
                     /translation="MPEQTQRDEKDAPLRRDIRALGDALGRAIQQHSRPAAFETEERL
                     RLGCRRLRECYELLQHTTGEEAESLREEIAALDQEITRVVEECDVETAIDVIRAFTVY
                     FHLVNAAEQHHRTRRRRVYDTAGEAAAQRGSLASLVALLQRSELDTDAIQVLLNRLTI
                     ELVFTAHPTEATRRSLILKSSRLAELLAEHDLFDVKTPREQAQWQREIEHVVTLLWRT
                     DSVLHVRLHPLDEIKMGLYYLEEILYDAIPELYTELEDLLSTHYRASSLCVPPFLRTG
                     SWIGGDQDGNPNVGPETLLQALRLQRARVIDRYRASIEELAQEYSQSLSYCTITPALA
                     DSIARDAALMSDYYRELGPQTAQEPYRLKLSFMWKRLGATQGSDLAGEQAQGSEEATD
                     SARIAYSHASDFLADLQTLLDSLQADGEREVAKGRLATLLRQAQVFGFHFAALDVRQH
                     SERHAAALAEQLQATSLLQGDYRSFDEVERVRLLGNLLRDPRVLPRRQLRLSDETRHV
                     LATFDAIRQAREEYGEQAVSCYIISMSRSLSDLLEVQFFCKEAGISALPIVPLFETID
                     DLRGCTSILESAFTHPLYREYVRGCDERQQVMLGYSDSSKDG"
BASE COUNT          398 a          625 c          591 g          406 t
ORIGIN      
        1 ccatccttgc tgctgtcgga atagcccagc atcacctgct ggcgttcgtc gcagccgcgc
       61 acatactcgc gataaagcgg gtgcgtaaag gcgctttcca ggatactcgt gcagccgcgc
      121 aggtcatcga tcgtctcgaa caacggcacg ataggcagcg cgctgatacc cgcctctttg
      181 cagaaaaact gcacttccaa cagatcgctc aggctgcgtg acatgctgat gatgtagcag
      241 gacaccgcct gctcgccata ttcctctcgc gcctgccgga tggcatcgaa ggtcgccagc
      301 acatgccgcg tctcatcgct caatcgcaac tggcggcgcg gcaacacgcg cgggtcgcgc
      361 agcaggtttc ccagcaggcg cacccgctct acttcatcga acgagcggta atcaccttgc
      421 agcagactcg ttgcctggag ctgttcggcc agcgccgcgg cgtgacgttc gctatgctgg
      481 cgcacgtcga gcgcggcaaa atggaaaccg aagacttgcg cctgtcgcag cagcgtcgcc
      541 agccgccctt tcgccacctc gcgctcccca tccgcctgca aactgtcaag caaggtttgc
      601 agatcggcca ggaaatcact cgcatggcta tatgcaatcc gcgcggagtc ggtcgcttcc
      661 tcagaccctt gcgcctgttc tcctgcaaga tcactaccct gagttgctcc caggcgtttc
      721 cacatgaacg agagcttaag ccggtacggt tcctgggcgg tctgcggtcc aagctcccgg
      781 tagtagtcgg acatcaacgc tgcatcgcgg gcaatagaat cggccagggc gggcgtaatc
      841 gtgcaatagc tgagcgattg cgaatattct tgcgccagtt cctcgattga ggcgcggtaa
      901 cggtcgatca cgcgcgccct ctgcaggcgc agggcttgca gcagcgtctc cggtcccaca
      961 ttggggttgc catcctggtc gcctccaatc cacgagccgg tgcgcaggaa cgggggaacg
     1021 cagagcgatg aagcgcgata atgcgtcgag agcaaatctt ctaactcggt atacaattca
     1081 ggaatggcgt cgtacaggat ttcttcgaga taatatagtc ccattttgat ttcgtcgagc
     1141 gggtgcaggc gcacgtgcaa caccgagtcg gtgcgccaca gcagcgtgac aacatgctcg
     1201 atctcgcgct gccactgcgc ctgctcgcgc ggcgtcttca cgtcgaacag gtcatgttcg
     1261 gcgagcaatt ccgccaggcg gctcgatttg aggatcaggc tgcggcgcgt cgcctcggtg
     1321 ggatgcgcgg tgaaaacgag ctcgatggta agccggttga gcaatacctg gatagcatcc
     1381 gtatccaact cactgcgctg gagcaaggcc acaagcgaag ccagggagcc gcgctgggca
     1441 gcagcttctc cagcggtatc gtacacccta cggcgacgag tgcggtgatg ctgttcggcg
     1501 gcgttgacca ggtggaagta gacggtgaag gcgcgaatga catcgatggc ggtctcaaca
     1561 tcacattcct cgaccacgcg cgtaatctct tgatctaacg ccgcaatctc ctcgcgcagg
     1621 ctctccgcct cctcgcccgt cgtgtgctgc aacagctcat agcactcgcg cagtcgcctg
     1681 catcccaggc gcagccgctc ctccgtctca aaggccgccg gtcggctgtg ctgctggatg
     1741 gctcgcccca gcgcatcgcc cagcgcgcgg atatcgcggc gcagcggcgc atctttctca
     1801 tcgcgttgtg tttgttcagg catgtagaaa agccaactta attttctcgg tcagaggtgg
     1861 tttccaatta gaaccgggca acgttgccat ccagcttgta cgcatcatag catgtcgagt
     1921 ctcttacgac aagtatggtt gtattttata gcagggctgg tgcaaggtcc agtgaggcat
     1981 ggcgaccgca agggatcgcc actacgataa cgcggcctgg
//
LOCUS       DASZAK010000197         1881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_466691, whole genome shotgun sequence.
ACCESSION   DASZAK010000197 DASZAK010000000
VERSION     DASZAK010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466691"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..538
                     /locus_tag="VGT44_03350"
     CDS             29..538
                     /locus_tag="VGT44_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2579867.1"
                     /translation="MELSEKIKHLREVEGELRGLGRAMTQTEVVKAMEDELQETISQA
                     YLSQLEKGRRVHLTANTRDLLARFFKVQPGYLVSDLEDYSTDLLTDLADDTDRLHTWL
                     AASAEDWRIEPPIADFFERLANVENPRLYLMLFRELLTMPAETIESLVGGSQASNGDA
                     SGELRVGIQ"
     gene            632..1462
                     /locus_tag="VGT44_03355"
     CDS             632..1462
                     /locus_tag="VGT44_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579868.1"
                     /translation="MIATDPLSLVFFACFLVGLLFFVVTALLGNLGHGATHTSATHQI
                     QAAVGHAAAHGGFHAGQAGHVGSQASHATSHAGTQNGQGRHAVSQHGGALSFLNPTSV
                     MLFLLGFGFFGYLFHNFVSFETPILTLIVAALGGIVVAALLLLAITRLFGNSEGATVQ
                     DVSDRTGLLGKVSLTIQENGIGEILYVSPGGMRKSVPARSIDGRRLEREQEVVVVNYS
                     DGVAEVDTWDHFIHEEGSYPEVTGKTATDSLEQLRTLLDEASKPDSQQLVIRNDVQME
                     "
     gene            1479..>1881
                     /locus_tag="VGT44_03360"
     CDS             1479..>1881
                     /locus_tag="VGT44_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEV2579869.1"
                     /translation="MDPLTLTIAGVVAIVIVVIVLIQTVGRLLRKVGPNQALIVFGAG
                     GTRVITGGSHFVIPLYQRAQEFSLELMSFDVAPSQDLYTSQGVAVNVEAVTQIKVRSD
                     DESVKTAAEQFLSKTQQQREALIRLVMEGHLR"
BASE COUNT          402 a          515 c          561 g          403 t
ORIGIN      
        1 caaatagcta tgcgaggtat gagagacaat ggagctatca gagaaaatca aacatcttcg
       61 cgaggtcgaa ggcgaactgc ggggattggg ccgcgctatg acccagaccg aggtggtcaa
      121 ggccatggag gacgaactgc aggagacgat cagccaggcg tacctctcgc agttggagaa
      181 aggcaggcgg gtgcatctca ccgccaatac gcgcgatttg ctggcgcggt tcttcaaagt
      241 gcagccgggc tatctggtca gcgacctgga ggattacagc accgatctgc tgaccgatct
      301 tgccgacgat actgaccgcc tgcatacctg gctggctgcc agcgcggagg attggcgcat
      361 cgaaccaccg attgcggact ttttcgagcg gctcgctaat gttgagaatc cgcgtctcta
      421 cctgatgctc tttcgtgaat tgttgacgat gcccgcggaa acgattgaat cgctcgtcgg
      481 cggttcgcag gcaagtaatg gtgatgcttc cggcgaatta cgtgttggaa ttcaataacg
      541 tcgttacgtc tatatatgat ggacaacctg taccaatcgg acatgtccga tttctggcag
      601 atggcacaca ctcggaggag ggacacgtgc tatgattgcg actgatcccc tatcactggt
      661 attcttcgcc tgttttctgg ttggcttgct cttctttgtc gtcaccgcgt tgttgggaaa
      721 cctgggacac ggagcgacac acacgagcgc cacgcaccag attcaggcag cggtaggcca
      781 tgccgccgcg catggaggtt tccatgcagg gcaggcaggt cacgtaggga gccaggcttc
      841 ccacgccaca tctcacgcgg gaacacagaa cggacagggc cgccacgctg taagccagca
      901 tggcggggcg ctatccttcc tgaacccaac ctcggtcatg ctgttcctgc tcggttttgg
      961 ctttttcggc tacctgttcc acaacttcgt cagcttcgag acccccattt taacgctgat
     1021 cgtcgccgct ctcggcggca tcgtagtggc tgcgctgctg ctgctggcga tcacgcggct
     1081 tttcggcaac agcgaggggg ccaccgtgca ggatgtctcg gatcggacgg ggctgcttgg
     1141 gaaggtcagt ctgactattc aggaaaacgg catcggcgaa atactgtatg tgtctcctgg
     1201 cgggatgcgt aagagcgtcc ccgcgcgcag catcgatggc cgccgcctgg aacgcgagca
     1261 ggaagtggta gtcgtcaact atagcgacgg ggtcgccgag gtcgatacct gggatcattt
     1321 tattcacgag gagggaagct acccggaagt gacgggcaaa acagccactg atagccttga
     1381 acaacttcgc acactgcttg atgaagcaag caaaccagac tcgcaacaac tcgtaatacg
     1441 caatgatgtt caaatggagt aagaaggagt aaaactatat ggacccattg acgctcacca
     1501 tcgccggtgt tgtcgccatc gtcatcgtgg tgattgtgct tattcagacg gtcgggcggc
     1561 tgctgcgcaa ggttggcccg aatcaggccc tgattgtgtt tggggcgggc ggaacgcgcg
     1621 ttatcaccgg cggctcgcat tttgtcatcc ccctgtacca gcgtgcccag gaattctcgc
     1681 ttgagttgat gtccttcgac gtagcgccct cgcaagacct gtatacctca cagggcgttg
     1741 ccgtcaatgt cgaagccgtt acgcagatta aagtgcgttc cgacgacgag agcgtcaaga
     1801 ccgccgccga gcagttcctc agtaagacgc agcagcagcg tgaagccttg atccggctgg
     1861 ttatggaagg ccacctgcgc g
//
LOCUS       DASZAK010000198         1604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_288504, whole genome shotgun sequence.
ACCESSION   DASZAK010000198 DASZAK010000000
VERSION     DASZAK010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288504"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1494)
                     /locus_tag="VGT44_03365"
     CDS             complement(<1..1494)
                     /locus_tag="VGT44_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579870.1"
                     /translation="MSQIAKSPCDEGVIQTGKLALPATRVNGNWVLVATILGSSMAFI
                     DSTVVNVALPVLQTELRATVADVQWVVEAYALFLSALILVGGSLGDHYGRRRVFATGI
                     VVFTLASVVCGFAPNITLLIAARAIQGIGGALLVPGSLAIISASFESERRGRAIGTWS
                     GFTSITSVLGPVLGGWLVQYASWRWVFFINVPLAAVVLAVLFWKVPDSRDDEEARQKL
                     DLWGALLATLGLGGVVYGLIEANNVGLGDPQVLLALAVGVAALVAFLLVEARSQFPMV
                     PLQLFRSPTFSGANLLTLLLYAALGGALFFVPFDLIRVQGYSPTAAGAANLPFIFIMF
                     TLSRWSGGLVTRYGAKLPLIIGPTIAAAGFVLYALPGVGGSYWTTYFPAIVVLGIGMA
                     ISVAPLTTAVMGAVDARRSGIASGINNAVARTAGLLAVAALGLVALGIFSASLDSQLA
                     SLPISPAIRAALLAQKSKLVGIDIPNGLSSVTHAAVQQAIDASFITSF"
BASE COUNT          374 a          544 c          446 g          240 t
ORIGIN      
        1 aaagctggtg atgaaagacg catcgatagc ctgttgcacg gcagcatggg taacgctgct
       61 caggccgttg gggatatcga tcccgaccag cttgctcttt tgcgccagga gtgcagcccg
      121 aatggcaggc gaaatgggaa gcgatgccag ctggctgtcg aggctggcgc tgaagatccc
      181 cagcgcaacc aggccgagcg ccgctaccgc cagcaatccg gcggtgcggg caaccgcgtt
      241 gttgatgccc gacgcgatgc ccgatcgccg cgcatccacc gcgcccatca ccgccgtcgt
      301 gagcggcgca acgctaatcg ccatcccaat acccagtacc acgatcgccg ggaagtaagt
      361 cgtccagtaa cttcctccaa cgccgggcag cgcatacagc acaaaaccgg cggcagcaat
      421 cgtagggccg atgatcagcg gcagtttcgc cccatagcgc gtcactaacc cgcccgacca
      481 gcgcgagagc gtgaacatga taaagatgaa cggcagattc gcggccccgg cagccgttgg
      541 cgaatagccc tgcacacgaa tcaggtcgaa tggcacaaaa aacagcgcgc cccccagcgc
      601 cgcgtaaagc agcagcgtca gcaaattcgc gccactgaac gtgggcgagc gaaacagttg
      661 cagcggcacc atcggaaact ggctgcgcgc ctccaccagt agaaaggcta caagcgctgc
      721 gacgccaacc gccagcgcca gcaagacctg cggatcgccc aggccgacat tgttcgcttc
      781 gatcaggccg tagaccacgc cgcccaggcc aagcgtggcc aacagcgcgc cccacaggtc
      841 cagcttctgc cgtgcctctt catcgtcgcg gctatcaggc actttccaga acaagaccgc
      901 gaggacaacg gcggcaagcg gcacattgat gaagaacacc cagcgccacg aggcatactg
      961 caccagccag ccgccaagca ccgggcctaa caccgaggtg atggaagtaa agcccgacca
     1021 cgtgccgata gcgcgcccgc gccgctcgct ctcgaacgag gcgctgatga ttgccaggct
     1081 gccaggcacc agcagcgcgc cgccgatgcc ttgaatagcc ctggcagcga tgagcagcgt
     1141 aatgttcggc gcgaaaccgc acaccaccga cgcgagcgta aaaaccacaa tgccggtggc
     1201 gaaaacgcgc ctgcgcccgt aatgatcgcc cagcgacccg cccaccagga tcaacgcgga
     1261 gagaaacagc gcgtatgcct cgaccaccca ctgcacatca gcaaccgtcg ccctcagttc
     1321 agtctgtagc accggcaagg cgacattgac caccgtgcta tctataaagg ccatactcga
     1381 accaagaata gtagcaacca gcacccagtt gccgttcact ctcgtcgcag gcagggcaag
     1441 tttgcccgtc tgtatcacgc cctcgtcgca cggcgacttc gcaatctgac tcatacttcc
     1501 ctcgtaatgt tgggtattct catcatcatc gtaatatcgg gtaccccgta ggtgccgatt
     1561 gatcgcgcac atcgccgatt gatcggcctg aacgcttgcc attg
//
LOCUS       DASZAK010000199         1581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_644575, whole genome shotgun sequence.
ACCESSION   DASZAK010000199 DASZAK010000000
VERSION     DASZAK010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644575"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VGT44_03370"
     CDS             complement(<1..324)
                     /locus_tag="VGT44_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579871.1"
                     /translation="MERSQRLHKAQDFIWRNARLVERYLFTSLFADGSREPVLAALRA
                     YQNEDGGFGNALEPDKRCPSSQPQDVEFALHLLDAIDAMDDAIVGRACDYLVTITTSE
                     GGVPYA"
     gene            complement(442..1368)
                     /locus_tag="VGT44_03375"
     CDS             complement(442..1368)
                     /locus_tag="VGT44_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEV2579872.1"
                     /translation="MNRIDRLTAIILLLQRGRRTASEIARRFEVSKRTVFRDMQALGE
                     IGVPIVTEPGAHGGYTLMPDYSLAPLQLTLREAVLLRLALSSVSQLADTPFKYERESL
                     LAKMQALIPARQQQDTEHFLQTIHLDVPERNYATPFIEQLIESARTGQWLSVSYRSER
                     GASGQTILPRRLYSAAGFWYCEAYSHEHQEERTYRVDRFVAVSAARAPEHLEPPRPQL
                     PYGHSSHPEVRIRLTARGVLAMERDPHLGDAIQPLGEEGGLLCFRCPPAEYDWLVRTL
                     LSLGPDAEVLAPDTLRRRVQQAAQEIARRYNE"
BASE COUNT          333 a          418 c          487 g          343 t
ORIGIN      
        1 cgcgtaaggc acgccgccct cggacgtagt aatggtgacg aggtagtcac aggcacgtcc
       61 aacaatggca tcatccatgg catcaatggc atccaggaga tgaagagcga actcgacgtc
      121 ctgcggttga ctggaggggc agcgcttatc gggctccagc gcgttgccaa aacctccgtc
      181 ctcgttctga taggcacgaa gagcggcaag cactggctcc ctgctgccat cagcaaacag
      241 ggaggtgaac aggtaacgct cgaccaggcg ggcgttacgc cagatgaaat cctgggcttt
      301 atggaggcgt tggctccgtt ccatgggatt ttcctttctc gttgtcggtt ggttcttcag
      361 agtagtgagc acgctgtgat aacgagcagt gctatcatca caagataagt gtacagaagg
      421 aatggtgaca acgctatgtc actattcgtt gtagcggcgg gcgatttcct gggcggcctg
      481 ttgcacgcga cgccgcaagg tgtcaggggc cagcacttcg gcatcagggc cgagactgag
      541 cagtgtgcgc accagccagt cgtactctgc tggtgggcag cgaaaacaga gcagtcctcc
      601 ctcctcgcca agcggttgaa tggcgtcgcc caggtgggga tcacgttcca tagccaggac
      661 tccgcgcgcc gtgagccgga tgcgtacttc tggatgcgag gaatgcccgt agggaagctg
      721 cgggcgcggg ggctctaagt gttcgggggc cctggctgcg ctcaccgcga cgaagcggtc
      781 aacgcggtag gtgcgttcct cttgatgttc atgcgagtag gcttcgcagt accagaagcc
      841 cgcagcagaa tagagccggc gaggaaggat cgtttgccca gaggctcccc gttctgagcg
      901 atacgagaca ctaagccatt gccccgtgcg cgcgctttcg atgagctgtt cgataaacgg
      961 cgtggcataa ttgcgttccg ggacatcgag atgaatggtt tgcaagaagt gttccgtatc
     1021 ttgttgctgc cgcgcgggaa tgagcgcctg catcttggcg aggagcgact cgcgctcata
     1081 tttgaaagga gtatcggcca attgagagac gctgctgagc gccaggcgca gcaggacggc
     1141 ctcgcgcaag gtcaattgga gcggagccag ggagtagtcc ggcatgagcg tgtagccacc
     1201 gtgcgcaccc ggctcggtca caatcggcac acctatttcg ccgagcgcct gcatatcgcg
     1261 aaaaaccgtg cgtttcgaca cttcaaagcg gcgagcgatc tcgctggcag tacgcctgcc
     1321 tctttgcaag agcaagatga tggcggtcaa gcgatcaatg cgattcatgt cctttgctcc
     1381 atatgttcac gctcttttct ttttcgccca tttcacgttt aggcacggtc caatttggac
     1441 gaattgtcct tgaccgtttg aacagtcaca cagtagggct ggaccttgct ggtgggtagg
     1501 aactgtcacg ttcatttgaa ccatgcctta gatagagatg tacctggaat atactacgat
     1561 ataatgaggt tgtcgtagct t
//
LOCUS       DASZAK010000200         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_217983, whole genome shotgun sequence.
ACCESSION   DASZAK010000200 DASZAK010000000
VERSION     DASZAK010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217983"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VGT44_03380"
     CDS             <1..308
                     /locus_tag="VGT44_03380"
                     /inference="COORDINATES: protein motif:HMM:NF021915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HEV2579873.1"
                     /translation="HPLEHEHLREIVDIMVAHTRQRIAEQSITLSVTEPARHLLVEQG
                     YDPTYGARPLRRAVQRLLDDMLAESLLRGEINPGDTVTVDVEDGRLAAKVAITARLT"
     gene            complement(684..1124)
                     /locus_tag="VGT44_03385"
     CDS             complement(684..1124)
                     /locus_tag="VGT44_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV2579874.1"
                     /translation="MANLTRYNPFNEVVSLRDAMDRLFEDSFIPRAFNGVATRGANAN
                     LYETPEGFTLQIPMPGVNADAVEITAQQDTITLKWETKVTIPENATTHWSGFQSGQYQ
                     QSFSLPAAINSERAEASYADGILTLNLPKAEHVKARTVKVVAKK"
     gene            1514..2386
                     /gene="mtnP"
                     /locus_tag="VGT44_03390"
     CDS             1514..2386
                     /gene="mtnP"
                     /locus_tag="VGT44_03390"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HEV2579875.1"
                     /translation="MPQATIGVIGGSGLYHMEGMTDVEEVAVTTPFGDPSDVITVGKV
                     EGVSMAFLPRHGRGHRISPTEIPARANIWALKSLGVEWVISVSAVGSLREHIAPRDLV
                     IPDQLFDRTKSRVNTFYEGGIVVHCTFAEPFCPTLSKLLLESARELGDITVHAGGAYV
                     CMEGPLFSTKAESHTYRSWGMDLIGMTALPEAKLAREAELCYAIIACATDYDCWHESA
                     ESVTVEMVIGNLSANVANAQRILRSVAQKIPADRSANTCECPSALASAILTDRSRIPA
                     EVKEKYSLLIGKYL"
     gene            2482..2581
                     /gene="ffs"
                     /locus_tag="VGT44_03395"
     ncRNA           2482..2581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGT44_03395"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            2685..>3127
                     /gene="dnaX"
                     /locus_tag="VGT44_03400"
     CDS             2685..>3127
                     /gene="dnaX"
                     /locus_tag="VGT44_03400"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV2579876.1"
                     /translation="MTSQTLYRKWRSQTFDDLVGQEPIVETLKNELKSGNLSHAYLFT
                     GPRGTGKTSTARLLAKTVNCEHPHDGEPCNECVPCREIAAGNSLNVIEIDAASNRGID
                     NIRELRERVMVPPATGKYKVYVLDEAHMLTTEAFNALLKTLEEPP"
BASE COUNT          697 a          880 c          892 g          658 t
ORIGIN      
        1 ttcatcccct ggaacacgaa cacctgcgcg agattgtcga tatcatggtc gcgcacaccc
       61 ggcagcgcat cgccgaacaa tcgatcacgc taagcgtcac cgaacccgca cgccacttgc
      121 tcgtggaaca gggctacgat ccgacctacg gagcgcgccc gctccgccgc gccgtccagc
      181 gcctgctcga cgacatgctg gccgagtcac tcctgcgcgg tgagatcaat cccggcgata
      241 cggtgacagt ggatgtggag gatggcaggc tggcggcaaa ggtggctatc acagccagat
      301 tgacgtaact atacccaaat atgaatttcc tatggaaaat gttgtggctc attactcgga
      361 tagaaaaccg ggagagaggt tgtataagga ggagttcgag attctcataa ttcgatggct
      421 gattggtctt actctcaaaa tacaaaatac tatggaggaa cctgagcgaa cacttgcgct
      481 atcaggattc atggatgcca ttaaaggcgc agatgtgctc ttagaaagag agttatgata
      541 gtttacgtag aaacgaattt cattttggaa ctggccttta agcaggagca acatcaagca
      601 gcgaatagca ttcttgaact agcagaaaaa gggaagccga gtagtttgta agtactcggc
      661 tccctgtcaa tagttacgat ctactacttc ttcgccacaa cctttaccgt gcgggccttc
      721 acatgctcgg cctttggcag gttcagcgtc aggatgccgt cggcgtagct ggcctcggcg
      781 cgctcggagt tgatggcggc tggcagcgag aagctctgct ggtactggcc actctggaaa
      841 ccgctccagt gcgtcgtcgc gttctcaggg atcgtgacct tcgtctccca tttcagcgta
      901 atggtgtcct gctgcgccgt gatctcaacc gcgtccgcat tcacgccagg cattgggatc
      961 tgtaaggtga agccttctgg cgtctcgtac aggttcgcgt tcgcgccgcg agtcgcgacc
     1021 ccattgaagg cgcgcggaat aaaactgtcc tcgaagagcc gatccatcgc gtcgcgtaga
     1081 gaaacgacct cgttgaacgg attgtaacgt gtcaggttag ccataattct aatctccttc
     1141 tttacttgtg acttctgaag tattttcacg aacccacctg cctggcgggt tcgtatttgt
     1201 ttgtctgttg ttactatata tcagggtatg ttagaggaac gtaagcgaag tgtaagaaaa
     1261 ttgttagaaa tcttgatggg attgctatca tacttgtgga actcgacggt ggcatggctc
     1321 ttctgctaca atgcagccat gcagttcgtc aaggttgatc tggcctgtag gggcgcagcc
     1381 cacatggtcg cccgcctgcg acgatcccaa tgtaggttgt ccctcgggtg ggcgaccatg
     1441 tgggctgcgc ccctacaggc caaatggaga aagacaggta actagcagag aacagatcag
     1501 gaggtagtta tccatgccac aggcaacgat aggtgtgatc ggcggcagcg gtctttacca
     1561 tatggagggc atgaccgacg ttgaggaggt cgcggtaaca acgccttttg gcgaccccag
     1621 cgacgtgatt acggttggca aggtcgaagg cgtttcgatg gcctttctgc cgcgacatgg
     1681 acgcgggcat cgcatctctc cgacggagat tccagcgcgc gccaacatct gggcgctcaa
     1741 aagcctgggc gtcgagtggg ttatatcggt cagcgccgtc ggcagcctgc gcgagcatat
     1801 cgccccgcgc gacctcgtca ttcccgacca gctctttgat cgcacgaaga gccgtgtgaa
     1861 taccttctat gaaggcggca ttgtcgtgca ttgcaccttt gccgagccgt tctgtcctac
     1921 gctcagcaag ctgctgctcg aatcggcgcg cgagctgggc gatatcacgg ttcacgcggg
     1981 gggcgcttat gtttgcatgg aaggcccgct cttctctacc aaagccgaat cgcataccta
     2041 tcgcagttgg ggcatggact tgatcggcat gaccgcgctg cccgaagcca agctcgcgcg
     2101 cgaggccgag ctgtgctacg ccatcatcgc ttgcgccacg gactacgact gctggcacga
     2161 atcggcagaa tccgtcaccg tagaaatggt gatcggcaac ctgagcgcca acgttgccaa
     2221 tgcccagcgc atcctgcgca gcgtcgcgca gaaaatcccc gccgaccgca gcgccaacac
     2281 ctgcgaatgc ccatcagcgc tggcctcggc catcctgaca gatcgcagca ggataccggc
     2341 ggaggtgaaa gaaaagtaca gcttattgat tgggaagtac ctgtaggcaa ttgacattcc
     2401 tgcctggcag gagtacacta caggtgttcc acagaaatga tgggaacggg ccggttttct
     2461 tccggccctg ccgatcggcg taatcgggtc ctgcgcggca gatgtctatg aaccccgcca
     2521 ggttcgtgag gaagcaacgg taaatggacc gctctgggtg ccgcagggta gcctgattgc
     2581 gctgatcggc agggccgggc gaaaatcggt tttctctatc acagcctttt ttctccggcg
     2641 ggggttcgcg gcccgtcaca atgcatgcag gcgggagtcg gttcatgacc tcacagacac
     2701 tctaccgtaa atggcgctca cagacgttcg atgacctggt tggacaggag ccgattgtcg
     2761 aaacgctcaa aaacgaactg aaaagcggca atctgagcca tgcctacctg ttcaccggcc
     2821 ccaggggaac gggcaagacc agcaccgccc gcctgctggc caaaaccgtc aactgcgagc
     2881 atccccacga tggcgaaccc tgcaacgaat gcgtgccatg ccgcgagatc gcggctggca
     2941 actccctcaa cgtcatcgag atcgacgcgg cctccaatcg cggcatcgac aacattcgcg
     3001 aactgcgcga gcgtgtcatg gtgccacccg ccaccggtaa atataaggtc tatgtgctgg
     3061 acgaagccca catgctgacc accgaggcgt tcaatgccct gctcaaaacg ctggaagagc
     3121 cgcccga
//
LOCUS       DASZAK010000201         8216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_147172, whole genome shotgun sequence.
ACCESSION   DASZAK010000201 DASZAK010000000
VERSION     DASZAK010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8216
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147172"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="VGT44_03405"
     CDS             <1..882
                     /locus_tag="VGT44_03405"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEV2579877.1"
                     /translation="HTQGGTVILPHLPNPNGEPAAMIATGRVDAVEFLRHGAESPRLG
                     GMYNHTEYYRYLNGGYRLPLVGGTDKMSSDVPVGLYRTYVYIPPDQDFTHETWCAGLA
                     AGRTFMSGGPIISFSVDGHAIGDTLRLNGNGGMVEVEAWAESIFPIHTLEIIQQGRVV
                     ATTEERTGVRRLELKTRIKVDGHTWLAARVSGPGYTNPILHHDAWRRGVVAHTSPIYV
                     ACGSDWRLFDQETAQYMLTLIDGSLTYIRELSPRYQPGTVMHHHGEEDHEAYLERPFL
                     EARAAIHRRMHELGPAE"
     gene            1181..1636
                     /locus_tag="VGT44_03410"
     CDS             1181..1636
                     /locus_tag="VGT44_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV2579878.1"
                     /translation="MAGQVRSTRVPWFVPFFNPIARRLLAAGIPMGPNVLITVRGRKS
                     GLPRTTPLTIVEISGRRWLMAPFGEVNWVRNLRAAGRATITVGRRKEEVSAVELGPTE
                     AVAFFRDVLGPRLQRTRVGSWIVRNLDKVDIDNPVEAAEGRPVFELHPR"
     gene            1841..4585
                     /locus_tag="VGT44_03415"
     CDS             1841..4585
                     /locus_tag="VGT44_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEV2579879.1"
                     /translation="MMNIQRHEQQQRELQVKEMEIVLPFAAISRASLAVAGGKAANLG
                     ELTQAGFPVPSGFCVTTEAYALVAEEADFEPILAELAAVRSDDTMQLAKVAAAVRARL
                     LAAPMPERVGEAIRKAYGELEHDEPVPVAVRSSATAEDLPFASFAGQQETYLNIIGIE
                     ALLDAVRLCWASLWTDRAVSYRVSLGIDQRAVRLAVAVQRMVEADVAGVLFTADPLTG
                     RRRQAVIDANPGLGEAVVSGAVNPDHFVVNTTTGEIVERRLGDKRLVIRATAGGGTQR
                     VELTGGNTEACLSDEQIRTLAALGARVEAHYGAPQDTEWAIDPSGKLWLLQSRPITTL
                     YPLPDNAPKTDDILRAYFSLNVFQGVYRPLTPMGIAVFRLFGSAIAGVFRLPPRDPLR
                     GPSILVEAGGRLFIDVTAALRTAIGRKFLLAAAQVGEAPSGALFQQLMTDPRLSLLPT
                     PRWHLIRAIGSLLEKTRTPLYVLQALLWPSTVQRRMTQVRVWLQSLNDEAAAASVQQG
                     ADLTDRLTALEQRLPGRVTSVFRTIPVMMISGLGSYALSGRLLKGIATADEMRIVLRG
                     LPHNPTTEMDLELWALAERVQADAVAAQAVRETLPERLTQQYREGTLPTTLQHGLGEF
                     LHAYGHRGVAEIDLGLPRWSEDPTHILGVLANYLQLNEPELAPDVQFHRGAQEAEAMV
                     ATLTRRAWRKGRLRGVLVRFFLKRTRALAGFREMPKFCLVLLLASIRRSLLSIGKAME
                     QVGCLEHAGDIFFLTLAEARAALAGKDLHQIVRERRASYDREVQRRSIPRVLLSDGTE
                     PTVALQAGELAEGTLKGSPASPGRVMAQARVILDPTGARLEPGEVLVAPSTDPGWTPL
                     FLTAGGLVMEMGGSMSHGAVVAREYGIPAVVGVPGATERITTGQQITVDGSAGTVYLE
                     "
     gene            complement(4651..5469)
                     /locus_tag="VGT44_03420"
     CDS             complement(4651..5469)
                     /locus_tag="VGT44_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2579880.1"
                     /translation="MEKWICKTCGTQYPPSEQPPEACPICSDERQYVGYEGQQWTTLA
                     QMQNDGFRNEFKEHEPGLTGIGTIPSFAIGERALLVQSAQGNVLWDCMSLIDHETVAG
                     IERLGGLTAIAISHPHYYSTMVEWADRFDVPIYLHEADREWVMRPNERITFWWGETYR
                     LSDNLTLLRLGGHFPGGTVLHWKQGAGGKGALLSGDIIQVVADRRWVSFMYSYPNLIP
                     LPATEVQRIRDTIAPYQFERLYGAWFERVVTHDAHDAVLRSAERYIRALQSVRH"
     gene            5634..6026
                     /locus_tag="VGT44_03425"
     CDS             5634..6026
                     /locus_tag="VGT44_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2579881.1"
                     /translation="MLTKIGTITVQVSDQDKALAFYTQKLGFEKRSDMPMGPDQRWIE
                     VAPPGAQTRILLYKATPDAPGADSYEAAKASIGKSTGMVLEVDDIEATFAQLKSNGVP
                     IEEEPKQQPWGWWGVFADQDGNTYGVHQ"
     gene            6054..6476
                     /locus_tag="VGT44_03430"
     CDS             6054..6476
                     /locus_tag="VGT44_03430"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV2579882.1"
                     /translation="MPDNTPQTRADIIRQFLPSSPYVGHLGIQLTGMRPDEATLRLPF
                     SDSLITIGTTVHGGAIASLIDTAAMVAAWSDDFVPDDMRGTTVSLTVNYLAPAEREDI
                     QATARVLKRGRSLVYLDVEVHSVSGKNIAKGLVTYKLG"
     gene            complement(6482..7228)
                     /locus_tag="VGT44_03435"
     CDS             complement(6482..7228)
                     /locus_tag="VGT44_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HEV2579883.1"
                     /translation="MESETPRSEIPEQHEPNSPPPGSAPSSSSFGRIDREHPIGGEMS
                     DVLGGILKRARLPWLVQQHSRVPVLAIFSFINGCISIGLMSALALITHTPFIFPSLGP
                     TAFLFFYTPTAPSASPRNTIIGHTIGVLAGYFSLVVTGLTMAGPALSTGVTWPRVIAA
                     ALSLGLTSGIMVLLKSPHPPAGATTLIVSLGILIHPWQLFILMVAVVLLTLQAIGINR
                     LAGIPYPLWNPRQQPVEGAEKGESAGKHNS"
     gene            complement(7275..7622)
                     /locus_tag="VGT44_03440"
     CDS             complement(7275..7622)
                     /locus_tag="VGT44_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579884.1"
                     /translation="MSKKDEKYMTAHPHTQQWRVANPDDPPVQRPPQGLHGPLPGPNY
                     VGLPGRTAIVQGQVFIVAVIVIAQLWLITDALYELLSGRSSSLGWLALVSGLGFVLAL
                     IVSIWPRRRIEES"
     gene            complement(7619..8167)
                     /locus_tag="VGT44_03445"
     CDS             complement(7619..8167)
                     /locus_tag="VGT44_03445"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEV2579885.1"
                     /translation="MVQEVNPYEKQSTSEATEQLQGAREEWREEFPYPWDEDEVVTRR
                     DTLRFLLSGAGALFLATGALAIIGHLPEGPGVQALPVAKVGELAENDWRVFDFPDQYA
                     QGILINLPGKGLVAYSDVCTHLSCAVIYQGDGKHLHCPCHEGLFDATTGNVLAGPPTR
                     PLPLIQLAIHDGTIYAVKVIPR"
BASE COUNT         1802 a         2268 c         2382 g         1764 t
ORIGIN      
        1 cacacccagg gcgggacggt gattctgccg cacctgccta acccgaacgg tgagcccgcg
       61 gccatgatcg cgacgggacg tgtcgatgcc gtggaattcc tccgtcatgg agccgagtcc
      121 ccgcggctcg gtggcatgta caaccacaca gagtattatc gctacctgaa cggcggatat
      181 cgcttaccgc ttgttggtgg caccgacaaa atgtccagcg atgtaccagt ggggctttat
      241 cgcacctatg tctacatacc gccagaccag gacttcacgc acgagacgtg gtgtgccggc
      301 ctggcggcag gacgaacctt tatgagcggc gggcccatca tctcgttcag cgtggacggc
      361 cacgcgatcg gggacacgct acgcctcaac ggaaacggcg gaatggtcga agtggaagcg
      421 tgggcagagt cgatcttccc gatccatacc ctcgagatca tccagcaggg gcgcgtggtc
      481 gccacaacgg aggagcgcac aggcgtgcgc cggctggagt tgaaaacgag gatcaaggtt
      541 gacggccaca cctggctggc tgcccgtgtg agcggcccag gctacaccaa cccgatcctg
      601 catcacgatg cctggaggcg cggggtagtg gcacacacct cgccgatcta cgtggcctgt
      661 gggagtgatt ggcggctctt tgaccaggaa acggcgcagt atatgctcac attgatcgac
      721 ggtagcctga cctacatccg cgagctgagt ccacgctatc aaccggggac tgtgatgcac
      781 caccacggag aggaggatca cgaggcctac ctggaacggc cttttctgga ggctcgggcg
      841 gccatccacc ggcgaatgca cgaactgggg cctgcagagt agagccaggg agttggcaaa
      901 gagtaaacac aagcgttgaa tctgccaacg taaaactccc gaggaagacc gggagccacc
      961 ggaacgacac ccatgacatt tgtcatgccc acaacacgag gaatttcact tctcaagtgc
     1021 ccgaagcctc gactatactt tccctatcac atctatttgg cgcggtgtca aacttttcag
     1081 gctatttggc gaaggagagc agagatgtct gtgacaaact acataagttg gaactctgta
     1141 tggttcgtct ggttcttgaa gctcaggagg ttttaacatc atggcagggc aggtcagatc
     1201 cacccgtgtt ccgtggttcg tcccattctt caacccaatt gcccggcgcc tcttagccgc
     1261 tggcatcccg atgggtccca atgtgctcat caccgttcgc ggccgtaaga gcggcctgcc
     1321 tcgcaccact cccctgacga tcgtcgagat ctcaggcagg cgatggctga tggccccctt
     1381 cggcgaggtc aactgggtgc gcaatcttcg tgctgccggc cgcgcaacca tcaccgtggg
     1441 acggcgcaaa gaagaggtga gcgcggtcga gttggggccc acagaagcag ttgcattctt
     1501 tcgcgacgtc cttggtcctc gcctacagcg gacgcgggtc ggttcgtgga tcgttcgaaa
     1561 tctcgataag gtcgatatcg acaacccggt agaagcggcc gagggccgac ctgttttcga
     1621 gctccacccg cgttgaagcc gaaaagctaa cgagagggaa ttgataaaca agggtgagcg
     1681 tcacattttt gcgtgaggct tgacgtacac cctcggatgg aacactgcac ccatgacaac
     1741 agtgcacttc tatggtacgc ttatccatgg ggaatgatgt ataagccagt cagtgaaagt
     1801 atgatgatgc aggacagacg ttcccaggcc aggggagggc atgatgaaca tccagcgaca
     1861 cgaacaacag cagcgtgagt tgcaggtcaa agagatggag attgttctcc cttttgctgc
     1921 aatcagccgt gcctcattag cagttgctgg cggtaaggca gcgaatctgg gagagctgac
     1981 gcaggcaggg ttccctgtac cttccgggtt ctgtgtgacc accgaagcct atgcgcttgt
     2041 ggctgaggaa gctgacttcg agccaattct ggctgagtta gcagcagttc gttctgatga
     2101 cacgatgcaa ctcgctaaag ttgcggcagc ggtacgggcc aggctgctgg cagctccgat
     2161 gcctgagagg gttggggagg caatcaggaa ggcctacggg gaactggagc atgatgaacc
     2221 tgtcccagtg gctgtgcgat cctcggctac ggctgaagat ttgccctttg cgagctttgc
     2281 cggtcagcag gagacatatc tcaacatcat tggtatcgag gctctcctgg atgcggtacg
     2341 actttgttgg gcatcgcttt ggaccgatcg cgccgttagc tatcgcgtaa gccttggaat
     2401 cgatcaacgt gctgtgcgcc tggcagttgc tgtacagcgg atggtcgaag ctgatgttgc
     2461 tggtgtgctc ttcactgctg acccactgac cgggaggcgc cggcaggcag tgattgatgc
     2521 caatcctggc cttggtgagg ctgtcgtctc aggcgcagtc aatcctgatc acttcgtggt
     2581 gaatactacc accggtgaga tcgttgagcg gcgtctgggg gataagcgcc tggttattcg
     2641 ggctactgct ggtggtggga cgcagcgagt cgaattgacc ggtggcaaca cagaggcttg
     2701 tctctccgat gagcagatac gaacacttgc cgcattggga gcgcgggtcg aagcgcatta
     2761 tggagcgccg caggatacag aatgggccat tgatccatcg gggaagctct ggctcttgca
     2821 gtcgcgcccg ataacaacac tttacccgct gcctgacaac gcgccaaaaa cggatgacat
     2881 actgcgtgcc tacttctccc tcaatgtttt tcagggggtg tatcgtccat tgacgccgat
     2941 ggggatcgct gtgttccggc tcttcggctc tgcaattgcc ggggtgtttc gcctgccccc
     3001 ccgcgatcct ctgcgcggtc cgagtatcct tgtcgaagct ggcggtcgtc tcttcatcga
     3061 tgtcacagct gccctgcgca ccgccattgg gcgcaagttt ctgctggcag cagcacaggt
     3121 cggggaagcg ccctcggggg ctcttttcca gcagcttatg acagatccgc gactttcact
     3181 gctccctacg ccacgctggc atttgatccg cgccatcggc tcgctgctgg agaaaacgcg
     3241 aacgccattg tatgtcctgc aagcattgct gtggccgtca acagtccaga ggcgcatgac
     3301 gcaggtgagg gtatggttgc agtctctcaa tgacgaagcc gcagcagctt cggtacagca
     3361 aggggctgat cttactgatc gtctgactgc tcttgagcaa cgcttacctg gcagagtgac
     3421 gtctgtcttt cgaaccattc cggtgatgat gatcagcgga ttgggttcct atgctctgag
     3481 tggtaggctg ctgaagggta ttgccactgc cgatgaaatg cggatcgtgc tgcgtggact
     3541 gccgcataac ccgacgacag aaatggatct cgaactctgg gcgcttgctg agcgcgtaca
     3601 agctgacgct gttgcggcac aagcagttcg cgaaacgctg cccgagcgac tgacccagca
     3661 gtatcgtgaa ggcacactgc ccaccacact gcagcacgga ttaggggagt tcctgcacgc
     3721 gtacggacat cgtggcgtgg cagaaatcga cctaggattg ccacgctggt ctgaagatcc
     3781 cacccatatc cttggcgtcc ttgccaacta cctccaactg aacgaacctg aactggcacc
     3841 agatgtacaa ttccatcgtg gagcgcagga ggcagaagcc atggttgcta cactcacccg
     3901 acgagcctgg cgcaaagggc ggctgcgcgg tgtgcttgtt cgcttcttct tgaagcgtac
     3961 gcgtgcgctg gctggtttcc gcgaaatgcc aaagttctgc ctcgtcttgc tcctggcatc
     4021 gatacgccgc tccctcttgt ccatcggcaa ggcaatggag caggtcggat gcttagagca
     4081 tgccggggat atcttcttct taacgctcgc tgaggcacgc gctgcactcg cggggaagga
     4141 tctgcaccag atagttcgtg agaggcgagc cagttacgat cgcgaagtgc agcgtcgctc
     4201 aatcccacgg gtgctgctct ccgatggcac agagccaacc gtggcgttgc aggctggcga
     4261 gttagctgaa ggcacgctca agggatctcc agcttccccc ggaagagtca tggctcaggc
     4321 acgtgtcatc cttgatccaa ccggcgctcg attggagccg ggcgaggtgc tggtcgctcc
     4381 ctcgaccgat ccaggctgga cgccgttgtt ccttaccgct ggtggtctgg taatggagat
     4441 gggcggctcc atgtcgcatg gtgctgttgt cgcccgcgag tatggtatcc cggcagtcgt
     4501 tggtgtgccg ggcgcgacgg agcgtatcac gacaggccaa cagattactg ttgatggttc
     4561 tgccgggacg gtctatctag agtaagggca gcgtagccgt cccatgatag gctctcgcgg
     4621 aaagaacatg ggcaaagtcg ctccagttga ttagtggcgc acggactgca acgcgcgaat
     4681 ataccgctcg gctgagcgca ggactgcatc atgcgcatcg tgcgtcacga cccgctcgaa
     4741 ccaggctcca tacaaccgct cgaactggta cggcgcaatc gtgtcccgaa tacgctgcac
     4801 ctcggtagcg ggtaatggga tcaggttggg atagctgtac atgaaactga cccagcggcg
     4861 atcagcaaca acttgaataa tatcgcccga gagcagagcg cctttgccac ctgctccctg
     4921 cttccaatgc agaaccgtgc cgccggggaa atggcctccg agcctgagca gggtcaagtt
     4981 gtccgagaga cgataggtct ccccccacca gaaggtgatg cgctcgttgg gacgcatgac
     5041 ccattcccgg tctgcctcgt ggagatagat ggggacgtca aagcgatcgg cccattccac
     5101 cattgttgaa tagtagtgtg gatgtgagat agcgatggcg gtgagaccgc ccagtcgctc
     5161 gatgcccgcg accgtttcgt gatcgatcaa gctcatgcag tcccacagga cattgccctg
     5221 cgcgctttgc accagcaggg cgcgttcacc aatggcgaat gaggggatag tcccaatgcc
     5281 ggtcaggccc ggttcatgct ccttgaattc gttgcgaaat ccatcattct gcatctgagc
     5341 gagcgtcgtc cactgctgtc cctcgtatcc gacgtattgg cgctcgtcac tacatatcgg
     5401 acatgcctct ggtggctgtt cgctcggcgg atactgtgtc ccgcatgtct tacatatcca
     5461 tttttccata tgtacgcctc ctcgcttaaa gctgatacct ttagcatacc ccaaagccag
     5521 agaaaatgct ctatgcgaac gaaggataca cactacatct gatcgagttg taatcgggca
     5581 ttgaaatacg ttatgttata atgaaaacgt gttctaaaag aaaggagaag gatatgttga
     5641 ccaaaattgg taccatcacg gttcaagtca gcgaccagga caaggcactg gcgttttata
     5701 cgcagaaact cggtttcgag aagcgcagcg atatgcctat gggtccagat cagcgctgga
     5761 ttgaagtcgc tccgccgggt gcccagacca gaattctctt gtataaggcc accccagatg
     5821 cgcctggcgc agactcatat gaggcagcaa aagccagcat cggcaagtcc accggcatgg
     5881 tcctggaagt agatgatatt gaagctacct ttgctcaact caagtcgaac ggcgtgccta
     5941 ttgaagagga gcccaaacaa cagccgtggg gctggtgggg agttttcgct gaccaggatg
     6001 gcaataccta tggtgtgcat cagtaacgta cgcgagtaca gggaatgatg atcatgcctg
     6061 ataacacacc acagactcgt gcagatatca tacgccagtt tctcccatcc tcgccctatg
     6121 ttggccacct tggtatccaa ttaaccggta tgcggcccga cgaggcaacg ctcaggctcc
     6181 cattctccga ctcgcttatt acgattggca ctactgttca cgggggagcc atcgcttcgt
     6241 tgatcgacac cgcggccatg gttgcggcat ggtctgatga ctttgtgccg gatgacatgc
     6301 gtggtaccac cgtgagtctg accgtcaatt atctcgcacc agcggagcgc gaggatatcc
     6361 aggccacagc ccgtgtgctg aagcgaggac gcagcctggt ctacctcgat gtcgaagtgc
     6421 atagcgtctc ggggaagaac atcgcaaagg gactcgttac ttacaagctt ggctaagcgg
     6481 gtcaggagtt gtgcttacca gcgctttcac ctttttcggc accctcgact ggctgctgtc
     6541 gcggattcca taatggataa gggattcctg ccaggcgatt gatgccgatg gcttgcaacg
     6601 tcagcagcac aacagccacc atcagaatga agagttgcca gggatgtatt aagatgccaa
     6661 gagaaacgat cagcgttgtt gccccggcag gtggatgagg cgatttgagt agcaccataa
     6721 taccagatgt aagtcccagc gagagtgccg ccgcaataac acgtggccac gtcacgccgg
     6781 ttgacagcgc aggtcctgcc atagtcaaac ctgttactac gagactgaaa taaccagcga
     6841 gaacaccaat agtatggcca atgatggtat tgcgtgggga agcgcttggc gctgtgggtg
     6901 tataaaagaa aagaaatgca gttggtccga gtgagggaaa aataaaaggc gtgtgcgtga
     6961 tgagggctaa tgcagacatc agaccaatac tgatgcaacc attgataaag ctgaagatag
     7021 cgagaacagg cactcgagag tgttgttgta ccaaccatgg aaggcgcgca cgcttgagga
     7081 tgccgcccag gacatcggac atctcgccgc cgattggatg ctcgcgatca atgcgcccaa
     7141 aggaggagct gctcggagct gagcctggcg gaggactgtt gggttcgtgc tgctcgggta
     7201 tttctgacct gggtgtttcg gattccatgc gtttgttcca ttcctacaca agttgccatc
     7261 gttctagaga atctttatga ttcttcgatc ctgcgccttg gccaaatgga cacaatcagc
     7321 gcgaggacaa aacccaggcc gctgacaagc gccagccagc ccagactgct tgatcgtcct
     7381 gaaagcaatt catagagcgc atcggtaatc agccagagtt gggcgatcac aatgactgcg
     7441 acaatgaaga cctgcccttg cacaatagcc gtacgccctg gcagacctac atagttgggg
     7501 cctggcagag ggccatgtaa tccttgtggc ggtcgctgta ccggcggatc atcaggattg
     7561 gcaacacgcc actgttgtgt atgcgggtgt gcagtcatgt atttttcatc tttcttactc
     7621 atcgtggtat caccttcaca gcataaatcg tgccgtcgtg aatagccagt tggatcaaag
     7681 gcagcggacg tgttggaggt ccggccagga cattaccagt tgtcgcatcg aacagccctt
     7741 catgacacgg acagtggagg tgcttgccat cgccctggta gatcaccgcg caggagagat
     7801 gggtacacac atcgctataa gcaacaagcc ctttgccagg taaattgatg aggatgccct
     7861 gagcatactg atcgggaaag tcaaacaccc tccaatcatt ctctgccaac tcgccaacct
     7921 ttgccaccgg taaagcctgc actccaggac cctcaggcaa atggccaata attgccagcg
     7981 cacccgttgc caggaagagc gctcccgctc cgctgagtag aaagcgcaac gtatcgcggc
     8041 gagtgacaac ctcgtcttcg tcccaaggat agggaaattc ttcgcgccac tcctcgcgag
     8101 caccctgcag ttgctccgtg gcttcgcttg tactctgctt ttcgtatgga ttcacttctt
     8161 gtaccaacct actcctcttt cgagtacctg caaaaggttc tctcttgcgg atccaa
//
LOCUS       DASZAK010000202         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_573145, whole genome shotgun sequence.
ACCESSION   DASZAK010000202 DASZAK010000000
VERSION     DASZAK010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573145"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="VGT44_03450"
     CDS             complement(<1..944)
                     /locus_tag="VGT44_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579886.1"
                     /translation="MSNEGQQPPQQPQPPQNPQQAYSGWQYDQGQSGPGGPQPNAPYP
                     PRAPYAQPPQAAYPGQQPAFPPQGYTQQPQQPQQPPMPQQPPMPPQQPLPARFSQPIQ
                     DMGSSLGYGQGMDHQYFNAPQPSQQLPRQIRLQQLREDRMRRQQQRIGGGGSVNSVKD
                     FTNLLKNRLRPSSDRPKTPDAGAQPMPPYGPGGPNGQPYPQGQYMPIEMSPSLLAPPE
                     ELSPSLRRPPELPPILQHSPAALPIRERNGYIGQNAPLPASQHAQDTGMLQRARIGQA
                     TMILTGAFVASRVLGLLRTSMFAYVFGTSSTSDAFLQA"
     gene            complement(1075..2112)
                     /locus_tag="VGT44_03455"
     CDS             complement(1075..2112)
                     /locus_tag="VGT44_03455"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HEV2579887.1"
                     /translation="MFNLVALGVYYPGNSIMHRLQARTKLLLIVWFAVLFTIANQRFF
                     HFAPYLLAIALVFGSIALGGISPGYMWRRLRLLILFSVIGIIPTLLFFSSSDQTHPPL
                     GTYGPLLLPNGGLRLVIVGYGVALLLLLLAMFLPAMRNARQSRWFKRLRVLLIVLVVI
                     GAIFLWVTRDLSPAATLPLGPFILASDSVWLIFSLFVVFLSLYSFSLILTMTTSPIAL
                     IEGLTILLTPLRWLRLPVDDFALMMLIALRFIPTLLEEVELLVKAQLSRGADLAHGSL
                     RERIQSLAALFIPFVQGTLRRAADLAIALEARGYEVEGRQTLLHERALQRVDYVIIGM
                     VFVLTIGTLLI"
     gene            complement(2116..3009)
                     /locus_tag="VGT44_03460"
     CDS             complement(2116..3009)
                     /locus_tag="VGT44_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV2579888.1"
                     /translation="MSEAMNAAIITVEHLTHIYQAKASGPLQKAALVDITMQIDQGSC
                     VAIIGVTGSGKSTLVQHFNGLLRPTSGTVVVDGFDTTDKTRDLVLLRRRVGMLFQYPE
                     SQLFERTLYADVAFGPRRMRIGRREVRRRVLAALDMVGLPHAEYALRNPFELSGGQRR
                     RAALAGVLAMSPKILILDEPTVGLDAGGREEFYAYLARIRQEQQVTIVLVSHDMTEVA
                     ELADQIFVLRDGRLVMQGEPRAIFAQGEALRSFGLATPPLSELLALLRQKGLDIPPDI
                     FTLEEAFNLLRDRNMIPEKGV"
     gene            complement(3006..>3561)
                     /locus_tag="VGT44_03465"
     CDS             complement(3006..>3561)
                     /locus_tag="VGT44_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2579889.1"
                     /translation="IATVVEDDVAWALTVRGMPAAEIRERVAWAMEAVGITHLRGLPP
                     NKLSGGQRQRLAIAGVLALRPRCIVADEATSQLDPLSRQEIVDLLHHLNQVYGLTILH
                     ITHLLEEAALAQRIVVMEQGAIVMEGTPTSVFADLERLRRLKLAVPEPIALAARLRAS
                     GIAITPESLTVEAIAGDIAEELAR"
BASE COUNT          720 a         1073 c         1091 g          677 t
ORIGIN      
        1 aacgcctgca aaaaggcgtc cgaggtggaa gatgtgccga aaacataggc aaacatggag
       61 gtgcgcagca agcccagcac ccggctggcg acaaatgctc ccgtcaggat catagtggcc
      121 tggccgatgc gcgctctctg caacatgccc gtatcctggg cgtgttggct cgccggtagc
      181 ggcgcgttct gcccaatgta gccatttctt tctcgaatgg gcaaggcggc aggactatgc
      241 tgcaaaatcg gcggcaattc aggcggcctt cgcagggacg gcgaaagctc ttccggcggc
      301 gcgagcagcg aaggcgacat ctcgataggc atgtattgcc cctgcggata aggctgcccg
      361 ttgggtccgc cgggtccgta aggcggcatt ggctgagcgc cggcatcggg cgtctttggc
      421 ctatccgagg atggccgtag acgatttttc agcagatttg tgaagtcttt gacgctattg
      481 accgatcctc cgccgccgat gcgttgctgt tgtctgcgca tacgatcttc gcgcaattgt
      541 tgcaggcgta tctggcgagg tagctgctgc gaaggttgag gcgcattgaa atactggtga
      601 tccattccct gcccgtaccc aagcgacgag cccatatcct gaatcggctg cgaaaagcgc
      661 gccggtagcg gctgctgtgg cggcatgggt ggctgctgcg gcataggtgg ttgttgtggc
      721 tgctgaggtt gctgcgtata cccttgcggc ggaaaggcag gttgttggcc gggataggca
      781 gcctggggag gctgtgcgta gggggcgcgt ggagggtaag gggcattcgg ctgcggccct
      841 ccaggccccg attgtccctg atcatattgc cagccagaat atgcctgctg agggttctgc
      901 ggcggctgcg gttgttgtgg tggctgttgt ccttcgttac tcaacgttct cgctcggtct
      961 gtctagtcct tcgatacgac ctgctcccct cttcaggtat cgtctcatca aatgtggtca
     1021 ctatcatatc atactcttca tactctgtca cccgtgcata cgggtaatgc tcccctatat
     1081 taataacgtt cctattgtta gaacgaacac catcccgata atcacataat cgacccgttg
     1141 cagcgccctt tcgtgcagca gggtctgacg gccttcaacc tcgtaaccac gagcttcgag
     1201 cgctattgcc aggtcggccg cgcgccgcag tgtgccttgc acgaagggga tgaaaagcgc
     1261 ggcaaggctc tgtattcgct cgcgcaggct gccgtgcgcc aggtcggccc cgcgcgagag
     1321 ttgcgctttg accagcaact ccacttcttc caatagcgtc ggaatgaagc gcagcgcgat
     1381 cagcatcatc agggcgaagt catcgacggg caggcgcagc cagcgcagtg gcgtgagcag
     1441 gatcgtcagc ccttcgatca gggcaattgg gctggtcgtc atggtcagga tcagcgaaaa
     1501 cgaatagagc gaaaggaaga cgacgaacag cgaaaagatc agccacacgc tatccgaggc
     1561 gaggataaat ggcccaagcg gcagcgtcgc ggctggcgat agatcgcgcg taacccacaa
     1621 gaagatcgcg ccgatgacaa ccagcacgat cagtaggacg cgcaggcgct tgaaccagcg
     1681 cgattgccgg gcgtttcgca tagcaggcag gaacattgcc agcagcagga gcagcagcgc
     1741 cacgccatag cccacgatga ccagccgtaa tccgccattc gggagcagca gcgggccgta
     1801 ggtgccgagc ggtgggtggg tctggtccga actgctgaag aagagcagcg tggggataat
     1861 gccgatcacc gaaaacagaa tcagcaggcg cagtcgccgc cacatgtagc ctggcgagat
     1921 gccccccagc gcgatgctgc cgaaaaccaa cgcaatcgcg agcaggtagg gcgcgaaatg
     1981 gaagaaacgc tggttggcaa tcgtaaataa gacggcaaac cagacgatca ataggagctt
     2041 ggttctggct tgcaggcgat gcatgatact gttaccggga taatagaccc ccagcgcgac
     2101 caggttaaac atcgttcata ctcccttctc cggtatcatg ttcctgtcgc gcagcaagtt
     2161 aaatgcttct tccagggtaa agatatcagg cgggatgtcc agtcccttct ggcgcagcag
     2221 cgcaaggagt tcgctgagcg gcggcgttgc cagcccaaac gagcgcagcg cctcgccctg
     2281 ggcaaagatg gcgcgcggct ctccctgcat caccaggcgg ccatctcgca gcacaaagat
     2341 ctgatcggcc agctccgcga cctcggtcat atcgtgcgag accagcacaa tagtaacctg
     2401 ttgttcctgc cggatgcgcg caagataagc gtaaaactct tcgcgaccac cggcatcgag
     2461 tccaacggtc ggctcgtcga gaatcaggat ctttggcgac atggccagta cgcccgccag
     2521 ggccgcgcgc cgccgctgcc cgccgctcag ctcgaacggg ttgcgcagcg cgtactctgc
     2581 atgcggcaaa cccaccatgt cgagcgcggc cagcacgcgc ctgcgcactt cacgcctgcc
     2641 gatacgcatc cggcgcggcc caaacgcgac atcggcgtac agtgtgcgct cgaacaactg
     2701 cgactccggg tattgaaaca gcatcccgac gcgcctccgc agcagcacca ggtcgcgcgt
     2761 tttatcggtg gtatcgaaac catcaacgac gacggtgccg ctcgtcgggc gcaacagccc
     2821 gttgaaatgc tgcaccagcg tcgattttcc cgaacccgtc acgccgataa tcgccacaca
     2881 gctcccctgg tcgatttgca tggtgatatc aaccagcgcg gccttctgca gcggaccgga
     2941 cgctttggcc tggtagatat gcgtcaggtg ttcaaccgtg atgattgccg cgttcattgc
     3001 ctcgctcacc gcgccaactc ctccgctata tccccggcga tagcctcgac cgtcaacgac
     3061 tccggcgtga tagcgatacc gcttgcccgc aggcgagcgg ccagcgcgat tggttcgggt
     3121 acggcaagct tcaggcggcg caggcgttcc aggtcggcga aaacgctggt cggcgttcct
     3181 tccatcacga ttgcgccttg ctccatgacc acgatacgct gcgccaatgc cgcttcttca
     3241 agcaaatgag tgatgtgcag gatcgtcagg ccataaacct gattgaggtg atgcagcaag
     3301 tcgacgattt cctggcgcga gagcggatcg agctgcgagg tggcttcgtc ggcgacgatg
     3361 cagcgcgggc gcagcgccag cactcccgcg atggcgagcc gctgacgctg gccgccggag
     3421 agtttgttgg ggggcagccc tcgcaggtgc gtgatgccga ccgcctccat cgcccaggcg
     3481 acgcgctcgc gtatctcagc ggcgggcatc ccgcgcaccg tcaatgccca ggctacgtcg
     3541 tcttccacca ctgtcgcgat g
//
LOCUS       DASZAK010000203         3491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_573153, whole genome shotgun sequence.
ACCESSION   DASZAK010000203 DASZAK010000000
VERSION     DASZAK010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573153"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(852..995)
                     /locus_tag="VGT44_03470"
     CDS             complement(852..995)
                     /locus_tag="VGT44_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579890.1"
                     /translation="MQGKKGASQGSARVGLVIFGPQQGGQAIATVLQARHRQVSEQRD
                     CFA"
     gene            1005..>3491
                     /locus_tag="VGT44_03475"
     CDS             1005..>3491
                     /locus_tag="VGT44_03475"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2579891.1"
                     /translation="MGDWLTIERETVGLGIRSNLSIDVHDFLQNLATCKTHGHAANER
                     CTACVQPLSKAVALYAGDFLAGFSLRDSPDFDDWQFYQADTLRREFAAALEGLTYCLS
                     ALHNFEEAIMYARRWLAVDRLHEPAHRQLMLLNAWAGQRAAALHQYRECVQALESALS
                     VAPLEATTQLYQLIKENRIPPLPAPLLAPTSLNEDDRARMDVPPDHPESMDQPGQPPA
                     AGIPLVGRDDELALLIKKYSAIDDGGQIIVIEGEAGIGKTRLANELLSFARQQGAVVL
                     EAACYEGEAQLAYGPVVSLLRAALTQQSDLLQTVSSSSLSEATRLLPELAAMQPGVYL
                     PPPLDSPGAQLRFFEGIRQLLLALSKGSSAGVLFFDDLHWADTASLDLLSYLMRRLHE
                     QRICLVFTWRGKQLTRGTRLHSLFVESQRAGHTTTLLLSRLEEEEVRELAQSLLGGGA
                     PGNVAHRLYAETEGIPFFLIEYLNAIEQGVLHPEQNDWTLPGGVRDLLHSRLDAVDEV
                     GWQLLTTAAVIGRSFDFDTLREVSGRSEEETVTALELVIRQGLVEEEQRQGNLLMYDF
                     SHEKLRALVYEETSLARRRLLHRRIAEVLAGHTRGRRDSGPFAGQVARHYRLAGNDAA
                     AADYFQQAGAYARALYANTEALAHFQTALALGHPDIPGLHEAIGDVHTLLGEYSAALK
                     SYETAAALCASESLARIEQKLGTVHARRGEWELAESHFEAALHASYESDRSTLYADWS
                     LTAHRRGQTQQALELAHRALQLAETSQDRHALAQAHTMLGMLASHRGDAEAARHHLEQ
                     SLSLAEMLNDPGMRAAALNNLAQAYRGVGES"
BASE COUNT          734 a          976 c         1021 g          760 t
ORIGIN      
        1 ggaataccgg ctcagacgtt tggagatcca gcctgtcatc ctgagcgcag cgaaggatct
       61 cggtagcttg caagtcagag agactgtgtg aaaagaatcg tcgttcggcc tgtcgagcca
      121 cttctcgagt gtcactttga ctgcacatga gcgcttctca cacagtctca gatcctgagc
      181 gcagcgaagg atgacagaca gaaccccagc cctctatcct tgacaacgct aatattccac
      241 ttgtataatt taattaatcg gtcagtcaat aatctgatgg tgatgaaatg ccataaaatt
      301 tttaccgcgt attaattaac cgattaatca aaaggaggag aagatgtcgc taaatcgaat
      361 tcgtaccttt aagttgccaa atcgaataag ggtctttaat aagtatgtct tgaatcggct
      421 tttgcgcgtg tttgcggagc tttccctcgg cccgtttgcg gtccttcgtc atgttggtcg
      481 tcgaagcgga aagacgtatg agacggtaat ctgggtgtgg ccgcttggag aagggtttgt
      541 gattgcgtta acatatgggc cgggggtgga ttggtaccga aatatgcagg tcgcaggagg
      601 cggtacggtg atctggcata agcgggtgta tgacgttggg aaaccggagc caattgatac
      661 cgaaacagct ctgccagcgt tccctgcagc attcaggccg atctttcgta ggactggcat
      721 gcgggatttt gtgtggatga agtcatcgca tataataaat atgtaacgga ggtgatagta
      781 tcgctcgcct gtctctgtat ttatttggct ctccgcgcat cgaactcgat gatatcccgg
      841 ccagcataga tttacgcaaa gcagtcgcgc tgctcgctta cctggcggtg acgcgcctgc
      901 agcacagtcg cgatagcctg gccgccctgt tgtggcccga aaatgaccag tcccacgcgc
      961 gcgctgccct gcgacgcacc ctttttgcct tgcataaagc cctgatgggg gactggctta
     1021 ccattgagag agagaccgtt ggcctgggca ttcgctcgaa tctctctatt gacgtgcatg
     1081 attttctgca aaacctggct acctgcaaga cacatgggca tgcggcgaac gaacgatgta
     1141 cggcctgcgt gcagccgctt tcaaaggctg tggcgttgta tgcgggcgat tttctggccg
     1201 gtttcagcct gcgggatagc ccagattttg acgactggca gttttaccag gcagacacac
     1261 tacgccgcga atttgcggct gctctcgaag gtctgacata ttgcctgagc gcattgcaca
     1321 acttcgaaga agccattatg tatgcccggc gctggctggc tgtagatcgc ttgcacgagc
     1381 cagctcatcg ccagttgatg ctgctcaatg cctgggccgg gcagcgcgcc gcggcgctgc
     1441 accagtatcg cgaatgcgtc caggcgcttg aatcagcact gagcgtggcg cctttggagg
     1501 caaccacgca actgtaccag cttatcaagg aaaatcgcat tccgccttta cccgcgccac
     1561 tattggctcc tacaagcctc aatgaagatg atcgtgccag gatggatgtg cctcctgacc
     1621 accctgaatc gatggatcaa cccggtcagc cgccagccgc tggaattccc ctggttgggc
     1681 gagatgatga attggcgctg cttatcaaaa agtattccgc catagacgat ggcggacaaa
     1741 tcattgtgat tgaaggcgag gcgggcatcg gcaaaacgcg cctggcgaac gagttgctct
     1801 cctttgcgcg ccagcaagga gccgtcgtcc tcgaagctgc ctgctacgag ggggaagccc
     1861 agcttgcgta tggaccggtc gtgtctctgc tgcgagcggc tctaactcaa caatcggacc
     1921 tcctacagac cgtctccagt tcttcgctga gcgaggctac gcgcttgctg cccgaactgg
     1981 ctgccatgca accgggcgtg tacctacctc cgccgcttga tagcccaggg gcgcagctgc
     2041 gtttctttga aggcattcga caactcctgc tggcactatc taagggaagt tctgctggtg
     2101 tccttttctt cgacgatctc cactgggcgg atactgcatc gcttgacctc ttgagctatt
     2161 tgatgcggcg gctgcacgaa cagcgcatct gcctggtctt cacctggcgc ggcaagcagt
     2221 tgacgcgagg gacgcgcctg cattcgctgt tcgttgagtc ccagcgagcg ggtcacacca
     2281 caacgctctt actctctcga ctggaggaag aggaagtgcg tgaactggcg caatcgttac
     2341 tcggtggcgg cgcgccgggg aacgtcgccc accgtcttta tgccgaaacg gagggcattc
     2401 ccttctttct aatcgagtac ttgaacgcta tcgagcaggg tgtgctgcat ccagagcaaa
     2461 atgactggac attgcccgga ggcgtgcgcg acctgctgca ttcacgcctg gatgccgtcg
     2521 atgaggtagg ctggcaactg ctcacgaccg ccgccgtcat cgggcgctcc tttgattttg
     2581 ataccctgcg tgaagtaagc gggcgcagtg aagaagaaac ggtcacggca ttagagctag
     2641 tcatcaggca ggggttggta gaggaggagc agcgccaggg caatctgctg atgtacgatt
     2701 tcagccacga gaaactgcgc gccctggtgt atgaagaaac cagcctggcg cgcaggcgtt
     2761 tgctccaccg ccgtatcgcc gaagtactcg ctggtcatac gcgcgggcgg cgcgatagcg
     2821 gcccgttcgc cggtcaggtt gcccggcatt atcgcctggc cggaaacgat gccgccgccg
     2881 ctgactactt tcagcaggcg ggggcctacg cacgcgcgct ctacgccaat actgaagctc
     2941 tcgctcactt tcaaacagcc ctggctttag ggcatcccga tatccctggg ctgcatgagg
     3001 ccattggcga cgtacatacg ctcctgggtg agtatagcgc ggcgctcaaa agctacgaaa
     3061 cagcggcagc cctctgcgca agtgaatcgc tggcccgtat cgagcagaaa ctcggtaccg
     3121 tccacgcgcg acggggcgaa tgggaactgg ctgaaagtca ttttgaggcg gcgctgcatg
     3181 cctcatatga aagtgaccgg tcaacgcttt acgccgattg gagtttgact gcgcaccggc
     3241 gcggccagac tcaacaggcg cttgaacttg cccaccgcgc actgcaactg gccgaaacga
     3301 gccaggacag gcatgcgctg gcccaggcac atactatgct gggtatgctc gccagccacc
     3361 ggggtgacgc ggaggccgcg cgccatcatc tcgagcaaag cctgagcctg gcggagatgc
     3421 tgaatgatcc cggtatgcgc gcagccgcgc tcaacaacct cgcccaggcg taccggggcg
     3481 tcggcgagag c
//
LOCUS       DASZAK010000204         1674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_573157, whole genome shotgun sequence.
ACCESSION   DASZAK010000204 DASZAK010000000
VERSION     DASZAK010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573157"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..>1674
                     /locus_tag="VGT44_03480"
     CDS             108..>1674
                     /locus_tag="VGT44_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV2579892.1"
                     /translation="MDEEKIDEGDADFVLPGRKEELRGSRPGEMRVRIVRPARPEFRR
                     VSKGILEATEAAYTPQGRLGKATSLLKRALIGVPIATARADHERLTKFKALAVLSSDA
                     ISSVAYATEAILINLVAGGSQHLGLTLPISLVIIGLLCIVAISYRQTIPAYPNGGGSY
                     IVAKENLGTLAGLVAAAALLIDYVLNVSVSIAAGVQNLASLFGALAPYVVPLDIALVA
                     LMMILNLRGLRESGSIFALPTYFFIASAILLIVVGLVKALLLQHQPLIGDYAPTKAIE
                     PLSIFLILRSFATGCSAMTGVEAISNGVPVFQKPETRNARTTLTLMALILGSLFLGIT
                     LLATTYHVEANAAGNPTVIAQIASQVFSGPLVFLFPVFQLATLGILTLSAETSYADFP
                     RLASLLARDKFLPSFFGFRGDRLAFSVGIISLSVLSGLLLVIFGGNTNALINLFAVGV
                     FMSFTLSQGGMVRHWWRLRAEKKGWQRSMVINGTGAATTLLVALVISSTKFLEGAWIV
                     VLLIPMLVLMFFSI"
BASE COUNT          286 a          514 c          491 g          383 t
ORIGIN      
        1 caagcctaca cgtagttcct acaccctgat ttggctcaat taaagcatag cttcatcgta
       61 tacggtatga tagagtaaga gataaactcg acaagtggag aaagcaattg gacgaagaga
      121 aaatcgatga gggcgatgcc gactttgtgc tgccgggtcg caaggaggaa ttgcgcggca
      181 gcaggccggg tgagatgcgg gtacgcattg tgcgcccggc gcgccctgaa ttccggcgag
      241 tcagcaaggg catcctcgaa gcgaccgagg cggcctatac tccacagggc cgattgggta
      301 aagctacctc gttgctcaaa cgcgcgctga tcggtgttcc catcgccacg gcccgcgccg
      361 atcacgagcg tctgaccaag ttcaaggcgc tggcggtcct ctcgtctgac gcgatctcct
      421 cggttgccta tgcgacggag gctatcctga ttaaccttgt cgcgggcggc tcgcagcacc
      481 tgggattgac gctgcccatc agcctggtca tcatcggcct gctgtgtatt gtggcgattt
      541 cctatcgcca gaccatcccc gcctacccga acggcggtgg gtcgtacatc gtcgcgaaag
      601 aaaacctggg aacgctggcc ggcctggtgg cggcggcagc gctgctgatt gactacgttc
      661 tcaatgtctc ggtcagcatc gccgccggcg tgcagaacct ggcgtcgctc ttcggcgcgc
      721 tggcgcccta tgtcgtgccg ctcgacatcg ccctggtggc gctcatgatg atcctcaacc
      781 tgcgcggact gcgcgagtcc ggctccattt tcgcgctgcc gacctatttc ttcatcgcta
      841 gcgcgattct attgatcgtc gtcgggctgg tgaaggcgct gctgctccag caccaaccgc
      901 tgatcggcga ctatgctccc acgaaggcga tagaaccgtt gtcgatcttt ctaatattgc
      961 gttcgtttgc taccggctgt tcggctatga cgggcgtcga ggccatctcc aacggggtgc
     1021 ctgtcttcca gaagccggag acgcgcaacg cgcgcaccac gctgaccttg atggccttga
     1081 tcctcggctc gctgtttctg gggatcacgc tgctggcgac gacttaccac gtagaagcaa
     1141 acgcggcggg caacccgacc gtgatcgcgc aaatcgcttc tcaggtgttc agtggtccac
     1201 tggtgttcct gttcccggtg ttccagctcg ccacgctggg cattctcacg ctctcagccg
     1261 agacgagcta tgccgatttc ccacgcctgg catcactgct ggcgcgcgat aaattcttgc
     1321 cgagtttttt tggctttcgc ggcgaccggt tggccttttc cgtaggcatc atctccctct
     1381 ctgtcctttc cggcctgctg ctcgttatct tcggtgggaa caccaatgcc ctcatcaacc
     1441 tgttcgccgt cggcgtcttc atgtccttta cgctctcaca gggtgggatg gtgcggcact
     1501 ggtggcgtct gcgcgccgag aagaagggct ggcaacgcag catggtcatc aatggcacgg
     1561 gggcggcgac gaccctgctc gtcgcgctgg tcatctcctc gacgaaattt ctggagggcg
     1621 cgtggatcgt cgtcctcctg atccccatgc tggtcttgat gtttttcagc atcc
//
LOCUS       DASZAK010000205         1573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_76729, whole genome shotgun sequence.
ACCESSION   DASZAK010000205 DASZAK010000000
VERSION     DASZAK010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76729"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VGT44_03485"
     CDS             <1..683
                     /locus_tag="VGT44_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2579893.1"
                     /translation="EQVLSLLNRVSLLPADQFIRKYPHQLSGGQRQRVSIARALAAHP
                     EVLLADEPVSMLDVSIRLDMLNLLLRLKEEEHLALLYITHDIASARYFADETIVMYAG
                     QMVEGGPSEEVTQRPKHPYTQLLLSAAPDPDRMDPEKHGLRALPGRRGEIPSLIRPPS
                     GCRFHPRCPHAMEVCRQSFPKRTDLGGGRWTNCFLYGDGGTNLEQTTLDVLKTKTATP
                     GDDDGNSL"
     gene            complement(694..>1573)
                     /locus_tag="VGT44_03490"
     CDS             complement(694..>1573)
                     /locus_tag="VGT44_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV2579894.1"
                     /translation="VQAPNGVIDVVVDDEAEAVRVAKRYLSFVQGAIAEWTCADQRLL
                     RQLIPENRLRVYDIRRVIETLADQDSVLELRRQFGSGIITAFIRIEGRPLGLIANNPM
                     HLGGAIDADAADKAARFMQLCDAYGLPILSLCDTPGFMVGPEAEKTATVRHVSRMFVT
                     AGSLTVPYFSVVLRKGYGLGAQAMTGGSFHAPFFIVAWPSGEFGAMGLEGAVRLGYRR
                     ELEAISDARERQAKFEEMVADAYQRGKAINMASYLEIDDVIDPFETRRWIIQGLRSAP
                     ATPPSEGKRRPFIDTW"
BASE COUNT          305 a          526 c          465 g          277 t
ORIGIN      
        1 ccgagcaggt cttgagcctc ctcaaccgcg tcagcctgct gcccgccgac cagttcatcc
       61 ggaaataccc tcaccagctc tccggcgggc agcgccagcg cgtctccatc gcgcgcgccc
      121 tggccgcgca cccggaggtg ctgctggccg acgaacccgt ttcgatgctg gatgtctcga
      181 tccgcctgga catgctgaat ttgctgctgc gtcttaaaga agaagaacac ctggccctct
      241 tatacatcac tcacgacatc gccagcgcgc gctacttcgc cgacgagacc atcgtcatgt
      301 acgcggggca gatggtcgaa ggcgggccga gcgaggaggt cacgcagcgc ccgaagcacc
      361 cctacacgca gctgctgctc tctgccgcgc ccgacccgga ccgcatggac cccgaaaagc
      421 atggcttgcg cgccctgcca ggccgtcgcg gcgagattcc cagcctgatc cgaccgccct
      481 ccggttgccg cttccatccg cgctgtcccc acgccatgga agtctgtcgc cagagcttcc
      541 ccaaacgcac cgatctggga ggcggtcgct ggacgaactg cttcctctat ggcgacggag
      601 gcacaaacct ggagcagacg acgctggatg tccttaaaac taaaactgcc acgccgggtg
      661 atgacgacgg caacagtttg taggagcgaa tcctcaccag gtatcaataa acggacgccg
      721 tttgccttca ctgggaggcg ttgctggcgc tgaacggagt ccctggataa tccaacggcg
      781 cgtttcaaac gggtcgatta catcatcgat ttccaggtag gaagccatgt tgatcgcctt
      841 gccgcgctgg tacgcatctg ccaccatttc ctcgaatttc gcctggcgtt cgcgcgcgtc
      901 gctgatggcc tccagctccc ggcggtatcc gaggcgaacc gcgccctcaa gccccatcgc
      961 accgaactcg ccactgggcc aggcgacgat gaaaaaggga gcgtggaagc tgccgccggt
     1021 catggcctgc gcgccgagtc catagccctt gcgtaagacg acgctgaagt agggaacggt
     1081 gagactgcct gccgtgacga acatacgaga gacgtgccgg acggtcgcgg ttttctccgc
     1141 ctctggcccc accataaaac cgggtgtatc gcacaatgag agtatgggta ggccgtaagc
     1201 gtcacatagt tgcatgaagc gcgccgcctt gtccgcggcg tcggcgtcga tagccccgcc
     1261 gaggtgcatg ggattattgg caatcagccc cagcgggcgc ccctcgatgc gaatgaaagc
     1321 cgtaatgatg ccgctaccga actggcggcg caattccaga acggaatcct ggtcggccag
     1381 cgtctcaatc acgcggcgaa tgtcgtagac gcgcaggcgg ttctccggga taagctggcg
     1441 cagcaggcgc tgatcggcgc aggtccattc cgcaatcgct ccctgaacga atgacaggta
     1501 gcgtttggcc acccgcacgg cctcggcctc gtcgtcaacc accacgtcga tcacgccgtt
     1561 gggtgcctgc acc
//
LOCUS       DASZAK010000206         9129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_360526, whole genome shotgun sequence.
ACCESSION   DASZAK010000206 DASZAK010000000
VERSION     DASZAK010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360526"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..3577
                     /locus_tag="VGT44_03495"
     CDS             362..3577
                     /locus_tag="VGT44_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2579895.1"
                     /translation="MSVDLPEDQVANKSSSLTSGVAGSIKFDWIFLLLSGWLVTGVYV
                     DGWAHNHFNIIDTFFTPWHALLYSGFLAVTSFLVVALLRNHRKGYAWENALPPGYGLS
                     LLGIAIFGIGGVADYFWHKLFGFEVELATLLSPTHLLLAAGGLLIVSGPLRAGWARAD
                     IADAADAPGTWKSALRALYSRLPLILSLTFVLSDLSFITSYAHPFVTLPATAPFHSSG
                     LGQFSIYLNDLVEAGGVVSILLQTALLFGTTLVAMRRVRLPVGTFTIMLTINLSAISV
                     LVENFFLIPAAVLGGLVADALFWYFRPSIDRPDALRLFTFLVPVSLFSIYFLDLRLFK
                     SVWWPIQIWMGSIVLAGFTGLLLSYVFVSPLKQAAQAVSASFRLSRRKVIVGLASLSL
                     AIGGLIEVENLLMRQTSSQANRSPFTSGPLPFYLYAYTTLVTLNVSTLQYYASSAVNR
                     IAWSPDGKMIASGNADGTIQLWRAVRAAVASSAPIDTPLLTYRKHTGAVTDLAWLPGT
                     TFIASASEDGTVQLWNTLSGAAHLVYRGHSGRVFSVSAAPDGRQIASAGQDRTVQIWD
                     AATGLPISTYRGHTASVVAVRWSPEGERIASSSSDGHVRVWLASSGELLLTLKVDVGK
                     SSDLPVEWSPDARSLATVAGGNVSTWNASTGLLLDQYVISSGSQSSGALNMAWSPVGG
                     NVVTAQADGSVWVWAAPTPRNGLPNTDQAAIRMPSGNVTTLSGEGGGAIQSTIILSYG
                     GYNAAATSVACSPDGRYVVAASEDQTVQVLDTTVGMTLLTYANKNVLPSATWSSDGRR
                     IASAGNSGSILVWDAATGDTLLSYEEHPAKSSVTQIRWSPNDQQIASISSDGTMLVWN
                     AATGATLLRRLQSGQTYTGVTWSDDGKHLALVNQDGQIQLLDITTARTLHATPQHEGG
                     ASSAVWSLDGKRVAIINTNGYVQVWDALNGAALLAPLVKYGLVQAVSWSPDGRRIATS
                     QLSVVQVWDAGTGDIICTCDGAAYPITSISWSSDNRHLAFAGNQGVVVWDISTQTMLL
                     TYQGMNWDSTQVPVVTWSPDGRRIASSSALGSVQVWLALAE"
     gene            complement(3581..4366)
                     /locus_tag="VGT44_03500"
     CDS             complement(3581..4366)
                     /locus_tag="VGT44_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEV2579896.1"
                     /translation="MAEQDFYKPEESSSTDDEDYDYSREPEPDAHTLRSNEQRSRYFL
                     AAMCFLLALLCLSAYLNNLPWLNATLLQLGAVLFAATGIFFLLRGSPANHEQPSSPTE
                     QDEENHSAWLYENQHSEVAYHDEPTVNENGASEDEVEEDEPTPFEMLVQEALATIPPE
                     FQERMANVFVRVMYEPGEETLRRVGTKEGYTLLGLYEGVPLTAYGRQQAAHLETITIY
                     QHTIERYCHGDPDRIREQVRHTVLHEVAHHFGIDHDEMPIWIR"
     gene            complement(4391..6070)
                     /locus_tag="VGT44_03505"
     CDS             complement(4391..6070)
                     /locus_tag="VGT44_03505"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2579897.1"
                     /translation="MNSRENGKRQDSNDFIAESVDEQIESRLTRSVPGQQPGGARLLR
                     DLVAMSREDTASLDRIWQRLSSHQTFHNADHRIDDIEQTGQDEASAAEAHPLTPFKRR
                     LFRQRLGLIAAAACMLLLVGSLAVVLNLGHNLVARHPGTGGTAEALCTPTDKNTTAPA
                     PVVYAEKAKQSGLYVAGGQGIYRFDVQGGQAGHLQPLWVFKMNACTSFTPTTYPNSLS
                     HGNMKMFTPRITTGAVVGDNMVFFDVAEESGAYLYALDSQDGALAWKAKVGVSGTPLV
                     LANLVVVATTDNAGNPMIMALDARHGTIHWSHSYATSITNQSEGLGAVGDDRVFVSAS
                     NTIFALDGTSGKQVWSSDIESGQIISGTRYFDGTLYVTASFSCYNCEVLPGTSAAYAF
                     DPATGNQLWESQKVAGYPSPPTEAQGVVYFGAIDGSVHALRASDGQQLWQSHVTGEVR
                     GAPDIADGLVVVTAGPFLDPTQHGTSNGEIVALAAAHGDYKESIAFPNMGINDYSNPL
                     AVTNGYLYIGTATFVLYSVNAHNGRQIQQYALQEINGGTFFELSPYLTLVP"
     gene            complement(6063..6638)
                     /locus_tag="VGT44_03510"
     CDS             complement(6063..6638)
                     /locus_tag="VGT44_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2579898.1"
                     /translation="MDEAREAKATQREPAAEDSPLAALFQAHAPGLLNYVREHTGSRE
                     DAEDIVLGAFTAALESNILLTRSEKEQAAWLWRVAKNKLADYRRRAMRHASATLQAVE
                     ETIYVDDAWQPDELAMRGEAYAELREAVASLPALQQEIVRLRFGQGLRCKEIALRLNK
                     GEGAVRMLLSRSLNSLRRNYDRQAEGEIPDE"
     gene            7005..8870
                     /locus_tag="VGT44_03515"
     CDS             7005..8870
                     /locus_tag="VGT44_03515"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV2579899.1"
                     /translation="MTTTETNSQVQQWTQLAQQLRVDSIRCTTTAGSGHPTSSMSAAD
                     LMAVLMSSYLHYDFDNPTHPNNDHLIFSKGHASPLLYSMYKAAGAITDEELLTLRKFG
                     SRLEGHPTPILPWVDVATGSLGQGLPIGVGIALCGKYLDKLPYRVWALLGDSETAEGS
                     VWEALDKASHYNLDNLVAIIDVNRLGQRGETELGWNLEAYHARATAFGCHAIVIDGHD
                     YDEINAAFAEALQTSGKPSVIIAKTMKGKGVSFLENKEGWHGKALNQQQADQALAELH
                     AARSKTFPVQKPDESLQPATSGQKQALALPTYSEALATRKAYGDTLKALGAANPDVVV
                     LDGEVSNSTYAEEFKFAYPDRFFEQYIAEQQLVASAIGMGVRHKIAFASTFAAFFSRA
                     YDFIRMAAISRANIRLSGSHAGVSIGEDGPSQMALEDLAMMRAVYDSTVLYPCDANQT
                     ASLVAQMVDLPGITYLRTTREKTPVIYGPDEQFPIGGSKVVRSSDKDEVTIVAAGITL
                     HNALSAYDQLKSQGIRARVIDAYSVKPIDDETLFAAAEEAGNTIVTVEDHWPEGGLGD
                     AVLEVFTQRDGTLPRVVKLAVQSMPGSGTPDQLMEEAGISAHAIVLAVRALVRGV"
     gene            8929..>9129
                     /locus_tag="VGT44_03520"
     CDS             8929..>9129
                     /locus_tag="VGT44_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579900.1"
                     /translation="MGGVARGPGGAIESLHQQATRATQAPFPTPSPAPTDECLYRVRR
                     ERVTGWILWLHATHEGAHGQDDG"
BASE COUNT         1819 a         2687 c         2585 g         2038 t
ORIGIN      
        1 ggtgagtatg aggatggtta gagaaaccag tacacgcttg ctatgcatca ttggatattc
       61 cccatttctg cctggatttg ccgccaatgt ggcgcggcag gctgcgttgg aatggagtcg
      121 gcagtgtaat acttattgta acggctcccc gatatgagtt gtaacgctag atcagttgaa
      181 ataatcgcct gcttgtttcg gcgctgtcgg cttctacgca ataattgcat cgcgttcctc
      241 ctataaacca ctataccact gcgggtctta caaatcacca ttatcatcgt ctatactaga
      301 gttggagttt gatacgcgat gaaatatcta ccccttgaaa agatgacggg gagcggatca
      361 catgtctgtc gatttgccgg aagaccaggt tgcaaacaaa tcgtcgtccc tcacgagcgg
      421 agttgccggt agtatcaaat tcgattggat cttcctgctg ttgagcggct ggctggtgac
      481 gggggtctac gtggatggct gggcgcataa tcacttcaat atcatcgata cgttttttac
      541 accctggcac gcgctgctct attcgggctt tctggcggtg acgagctttc tggtggtagc
      601 gctgctcagg aatcatcgca agggatatgc ctgggagaac gcgcttcctc cggggtatgg
      661 cctctcgctg ctgggtatcg ccatctttgg cattggcggc gtggcggact acttctggca
      721 taagctcttt ggctttgagg tggagctggc aaccttgctc agcccgaccc atctgctgct
      781 ggcagccggg ggattgctga tcgtcagtgg accgctgcgg gcgggctggg cgcgagcgga
      841 cattgcagat gctgcggacg ctcctggaac ctggaaatct gcgctgcgcg ccctatactc
      901 tcgccttccg ctgatccttt cactaacatt cgttctctca gacctctcct tcatcacctc
      961 gtatgcgcat cccttcgtga cgctgccggc cacagcgccg tttcattcaa gcgggttagg
     1021 gcaatttagc atctatttga acgacctcgt cgaggcgggc ggcgtcgtga gtattttgct
     1081 gcaaaccgcc ctgctgtttg gcacgacgct ggtcgccatg cggcgcgtgc ggctgccggt
     1141 gggaaccttt accatcatgc tcacgatcaa cctctccgcc atcagcgtac ttgtcgagaa
     1201 ttttttcttg atccccgctg ccgtgctggg cggcctcgtc gccgacgcgc tgttctggta
     1261 tttcaggcca tccatagacc gccccgacgc gctgcgcctc ttcacctttc ttgtgccggt
     1321 cagtctcttc tcgatctatt ttcttgattt gaggctcttc aagagcgtgt ggtggccgat
     1381 tcagatctgg atggggtcga tcgtgctggc gggttttaca ggtctgctgc tcagctatgt
     1441 gtttgtttcg cccctcaaac aggcagctca ggctgtgtcc gcctcatttc gcctatctcg
     1501 ccgcaaggta atcgttggcc tggcgagctt gagcctggca atcggagggc tgatcgaggt
     1561 agaaaatttg ctgatgcggc aaaccagttc gcaggcaaac cggtctccat ttacgagcgg
     1621 cccgttgccg ttctacctgt atgcctatac gacgctggtc actctcaacg tctcaacgct
     1681 gcaatactat gccagcagcg cggtcaatcg cattgcctgg tcgcccgatg ggaaaatgat
     1741 tgcctctggc aatgctgatg ggacaatcca actctggcga gcagtacgcg cggcggtggc
     1801 atcaagcgcg ccaatcgata cgcctctcct gacctatcgc aagcacacgg gggcggtgac
     1861 tgacctggca tggctgcccg gaacgacgtt catcgcgtcg gccagtgagg acggcacggt
     1921 ccagctctgg aataccctga gcggcgctgc gcatctcgtg tatcgcgggc atagtggccg
     1981 ggtcttttct gtctccgctg cgcccgatgg caggcagatt gcctcggcgg ggcaggatcg
     2041 aacggtgcaa atctgggatg ccgcgacggg cctgcccatc tcaacctatc gaggccatac
     2101 tgccagtgtg gtagcggtgc gatggtcgcc cgaaggcgag cggattgcat cctcgagcag
     2161 cgatggacat gtgcgcgtct ggctggcatc atctggcgaa ctgcttctga cgttgaaagt
     2221 tgatgtaggc aaatcatctg acctgcctgt tgagtggtcg ccggacgctc gctcgctcgc
     2281 gacggtagct ggtggcaacg tgtcaacctg gaatgcctcg accggccttt tgctcgacca
     2341 gtatgtaatt tcgtcgggat cacagagcag cggcgcgttg aatatggcct ggtcgccggt
     2401 tggcggaaat gtggtcacgg cgcaggctga tgggtctgtc tgggtctggg ccgctcccac
     2461 tccgcggaac ggattgccta acacagacca ggcagcaata cgtatgccgt cgggtaatgt
     2521 gacgacgctg tccggtgaag gtggaggtgc tattcaaagc acgatcatac tctcctatgg
     2581 aggctataat gcggcagcga cttcggttgc ctgttcgccc gatggtcgat atgtcgtagc
     2641 ggcaagtgag gatcagacag tgcaggtgct ggatacgacg gtcggtatga cgctgctcac
     2701 ctacgccaat aagaatgtac tgccatccgc tacctggtct tccgatggca gacgcattgc
     2761 ctcggcgggc aatagtggct cgatcctcgt ctgggatgcc gcgacgggcg atacactgtt
     2821 gagctatgaa gagcatccag ccaaaagctc tgtcacgcag atacgctggt cgcccaacga
     2881 tcaacaaatc gcctccatca gcagcgatgg gaccatgctg gtctggaatg ccgcgacggg
     2941 cgcaacgctg ctcaggcgcc tgcaatccgg ccagacctat actggcgtaa catggtcgga
     3001 cgacggaaaa catctcgcgc tggtgaacca ggatggccag attcaactcc tcgatatcac
     3061 taccgccaga accctacacg caacaccgca acacgaggga ggcgccagta gcgctgtctg
     3121 gtctctcgac ggcaagcgtg tcgccattat caacacgaat gggtatgtcc aggtctggga
     3181 tgccctcaat ggggcggcgc tgcttgctcc actcgtaaaa tacggattgg tacaggctgt
     3241 ctcctggtcg ccggacggta gacgtattgc taccagccag ttatcggtgg tgcaggtctg
     3301 ggatgccggg actggcgata taatttgtac ctgcgatggc gcagcctatc ccatcacttc
     3361 catctcctgg tcgtccgata accggcacct ggcttttgca ggcaaccagg gagtggtggt
     3421 ctgggatatt tcaacgcaaa cgatgctgct gacttatcaa ggaatgaact gggattcgac
     3481 ccaggtaccc gtcgtcacct ggtcgcctga tgggagacgg attgcttcgt cgagcgcgct
     3541 cggctcggtc caggtctggt tagctctcgc ggaatagcgc ttaccgaatc cagatcggca
     3601 tctcatcgtg gtcgatgccg aaatgatgcg ccacctcgtg cagcaccgta tgtcgcacct
     3661 gctcgcgaat acggtcggga tcgccgtgac aatagcgttc gatagtatgc tggtagatcg
     3721 tgatcgtctc aaggtgcgcc gcttgctgcc ggccatacgc ggtcagcggc accccctcgt
     3781 acagtcctaa cagcgtatag ccttcctttg tgccaacgcg ccgcagcgtc tcttcgccag
     3841 gttcatacat cacacgcaca aacacgtttg ccatgcgctc ctgaaattcc gggggaatgg
     3901 tggccagcgc ctcctgcaca agcatctcaa atggcgtcgg ttcatcctct tcaacctcat
     3961 cttcactcgc gccattttcg ttcactgtcg gctcatcgtg atacgctacc tcgctgtgct
     4021 gattttcgta tagccatgca ctatgatttt cctcgtcctg ttcagttgga gaggatggtt
     4081 gctcgtggtt ggcaggggag ccacgcagga ggaaaaagat gccggtggcc gcgaaaagca
     4141 cggcacccag ttgcagaagg gtcgcattga gccaggggag attattgagg tacgcgctca
     4201 ggcagagcag cgccagcagg aaacacatcg ccgccaggaa atagcgcgag cgctgctcgt
     4261 ttgaccgcag cgtatgcgca tccggttccg gctcgcgaga ataatcatag tcctcgtcgt
     4321 cggtggagga cgactcttct ggtttataga agtcttgctc ggccatatct tctatatacc
     4381 acgtttggct ttatggcaca agcgtcaagt acggactgag ttcgaaaaat gtacccccat
     4441 ttatctcctg aagcgcgtat tgttgtattt gtctaccgtt atgggcattg acactgtaga
     4501 gtacaaacgt agcggtgccg atatagaggt acccatttgt gactgctaac ggattcgagt
     4561 agtcgttgat gcccatattt ggaaacgcaa tcgactcttt gtaatcacca tgagctgctg
     4621 cgagcgctac aatctctcca ttggaggtgc cgtgttgcgt cggatcgaga aacggccctg
     4681 cggtcacaac cacaagccca tcagcaatat ctggagcgcc gcgcacttcg ccggtcacat
     4741 gcgactgcca gagttgttgc ccatcgctgg cgcgcagcgc gtgaacacta ccatcgattg
     4801 cgccgaagta gaccacaccc tgcgcctctg ttggcggcga tggatacccg gccaccttct
     4861 gcgactccca cagctgattg ccggtagcag gatcgaaggc ataggccgcg ctggttccag
     4921 gcagcacctc gcagttgtag caggagaaag atgcagtgac gtagagcgtt ccgtcgaagt
     4981 agcgcgtccc actgattatc tggccgcttt cgatatctga actccatact tgctttccgc
     5041 tcgtgccatc aagcgcaaag atagtgttcg acgcgctaac aaagactctg tcgtctccca
     5101 ctgctccaag cccctcagat tgatttgtta tactggtagc gtagctatgg gaccagtgta
     5161 tcgtgccatg gcgcgcgtcg agcgccatta tcatcggatt cccggcatta tccgttgttg
     5221 ctaccacgac caggttggcc agcacaagtg gcgttcccga tacaccgacc tttgccttcc
     5281 atgccagtgc gccatcctgt gaatccagcg cgtagaggta ggctcctgac tcttcagcga
     5341 cgtcgaagaa gaccatatta tcgccaacaa cagcacccgt cgtgatacgt ggagtaaaca
     5401 ttttcatgtt gccatgagaa agtgaatttg ggtaagtcgt gggtgtaaaa gaggtgcagg
     5461 cattcatctt aaatacccag aggggttgta ggtgtccagc ctgacctccc tggacatcga
     5521 agcggtagat gccctgacct ccggccacgt acaggccgga ttgcttcgct ttttccgcat
     5581 acacgaccgg ggcgggcgcc gttgtgttct tatccgttgg cgtacatagg gcctcagccg
     5641 taccacctgt accaggatgg cgcgccacca gattatgccc caggttcagc acaaccgcca
     5701 ggctgcctac caacagcagc atacacgcgg ctgccgcgat gaggcccagg cgctgccgga
     5761 aaagtctcct tttgaatggg gtgagcgggt gcgcttcggc tgcgctggct tcatcttgtc
     5821 cggtctgctc tatatcgtct atacgatggt cagcgttatg gaaagtctga tgcgaagaga
     5881 gccgctgcca gatacggtcc aggctggctg tatcttcccg gctcattgcg accagatcgc
     5941 gcagcaaacg cgctccgcca ggctgttgtc ctggaactga tctcgttaac cgcgattcta
     6001 tctgttcatc aacgctctcg gcaataaagt cattgctatc ctggcgcttg ccgttttctc
     6061 tgctattcat cggggatctc tccttctgcc tgtcgatcgt agttacggcg caaggaattg
     6121 agggagcgcg agagcagcat acgcaccgct ccctcgcctt tattcagccg cagcgcgatc
     6181 tccttacagc gtaatccctg cccaaaacgc aggcgcacga tctcctgttg taacgcgggc
     6241 aggcttgcca ccgcttctcg cagctcggcg taagcctctc cgcgcatggc aagctcgtcg
     6301 ggctgccacg catcatcgac gtagatcgtc tcttcgactg cttgtaaggt tgcgctggca
     6361 tgtcgcatgg cgcgtcggcg gtaatccgcc agtttgttct tcgccacgcg ccacagccac
     6421 gccgcctgtt ccttctcgct tcgcgtcagc aatatgttcg attcaagtgc ggctgtaaac
     6481 gcccccagca caatgtcttc cgcgtcctcg cgcgagccgg tatgttctcg gacgtagttg
     6541 agcagccccg gagcatgtgc ctgaaataac gccgctagcg gcgaatcttc cgcagcaggt
     6601 tcgcgctggg ttgcctttgc ctctcgtgcc tcgtccatgc cgctcccttt tccgatcatg
     6661 catagtgaga tctcatactg tatatcgccg ccgggcgtaa aatgtcacat ctgtaacgaa
     6721 aaccgcgttt gctgatactt tgcttcagct tgctcctatt gtatccggca acctacaata
     6781 caacaggatg tgatgtataa tacagttgac atcgcccttt tatgagcttt aaataattag
     6841 cgaggcatcc tcgtaggtgc cgatttatcg cgcacaccgc cgattgatcg gctacggtgg
     6901 gcgtgtacag caggcattcg ggccgataaa tcggcaccta cagaggcgta tacccgcttt
     6961 actatttaaa attcttcaag cggggcagaa aatgaggaga gtatatgacg acaaccgaga
     7021 cgaattccca ggtacagcaa tggacgcagc ttgctcagca gttgcgcgta gatagcattc
     7081 gatgcacgac tacggcgggg tcggggcatc ccacttcgtc gatgtcagcg gcggatttga
     7141 tggccgtgtt gatgagcagc tatttacact acgacttcga caatcctacg catcccaaca
     7201 acgaccacct gatcttttca aagggtcatg cctcgcccct gctctattcc atgtacaaag
     7261 cggcgggcgc gatcaccgat gaagaattgc tgacgctgcg caaattcggc agccgcctcg
     7321 aagggcatcc cacgcctata ttgccctggg tggacgttgc caccggctcg cttggccagg
     7381 gtctgcccat cggcgtcggc atcgccctgt gcggcaaata tttggacaag ctgccctatc
     7441 gcgtctgggc gctgctcggc gatagcgaga cggcggaagg ctccgtctgg gaggcgcttg
     7501 ataaggcgtc ccactacaac cttgataacc tggttgcgat catcgacgta aaccgtttgg
     7561 gtcagcgcgg cgaaaccgag ctgggctgga acctggaggc ctatcacgcg cgcgcgacgg
     7621 cctttggctg ccacgccatc gtcatcgatg ggcatgacta cgacgagatc aacgccgcct
     7681 ttgccgaggc gctgcagacc agcggcaagc cgagcgtcat tatcgcgaag acgatgaagg
     7741 gcaagggcgt ctccttcctg gaaaataaag aaggctggca cggcaaggcg ctcaatcagc
     7801 agcaggcaga ccaggcgctc gccgaactac acgccgcgcg cagcaagacc ttcccggtgc
     7861 aaaagcccga cgagagtctt caaccggcaa catccggcca gaagcaggcg ttggcgctgc
     7921 cgacctatag cgaggcgctg gcgacgcgca aagcctacgg cgatacgctg aaagctctcg
     7981 gagcggccaa tcccgatgtc gtcgtgctgg atggcgaggt cagcaactcg acctatgccg
     8041 aggagttcaa gttcgcctac cccgatcgct tcttcgagca gtatatcgcc gagcagcagc
     8101 tggttgcctc agcaatcggt atgggcgtcc gtcacaagat cgccttcgcc tccaccttcg
     8161 ccgccttctt ctcccgcgct tacgacttca tacgtatggc ggccatctcg cgcgccaata
     8221 ttcgcctgag cgggtcacac gcgggcgttt cgattggcga agacggtcca tcgcagatgg
     8281 cgctggagga cctggctatg atgcgcgccg tctacgacag caccgtgtta tacccctgcg
     8341 acgccaacca gaccgccagt ctggtcgcgc agatggtgga cctgccgggt atcacctacc
     8401 tgcgcaccac gcgcgagaag acgcccgtca tctacggccc cgacgagcag ttccccattg
     8461 gcggcagcaa agttgtgcgc tcttcagaca aagatgaagt gaccatcgtg gccgccggta
     8521 ttacgctgca taacgcgctg tctgcctacg accagctcaa gagccagggc atccgcgcgc
     8581 gcgtgatcga tgcctactcc gtcaaaccca tcgacgatga gacgctcttc gccgccgccg
     8641 aagaagcggg caacaccatc gtcaccgtcg aagatcactg gcccgaaggc ggcctgggcg
     8701 acgccgtcct cgaagtcttc acccaacgcg acggcacact accccgcgtc gtcaagctgg
     8761 ccgtccaatc tatgcccggc tcaggcaccc ccgaccaact catggaagaa gcgggcatca
     8821 gcgcccacgc catcgtcctg gccgtgcgcg ccctcgtgcg cggcgtgtaa ccactgtatc
     8881 caacctgtaa ccctctcccg tcttactctg tagaggcggg ggaggacgat gggtggagtc
     8941 gcccgcgggc ccggtggtgc gattgaatcg ttgcatcaac aggctacgcg ggcgacgcaa
     9001 gctcccttcc ccacgccctc ccccgcccct acggacgaat gcctctacag agtaagacgg
     9061 gagagggtta caggttggat actgtggtta cacgccacgc acgagggcgc gcacggccag
     9121 gacgatgga
//
LOCUS       DASZAK010000207         1922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_502397, whole genome shotgun sequence.
ACCESSION   DASZAK010000207 DASZAK010000000
VERSION     DASZAK010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1922
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502397"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..776
                     /locus_tag="VGT44_03525"
     CDS             <1..776
                     /locus_tag="VGT44_03525"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2579901.1"
                     /translation="LAGGVVAALFGVLVGFIALKRLRSDYMAIVTVAVGAIVYDFIGN
                     YTPLFNGWDGLGGVPNPLYTTVNLDFQTYQWFYVGLTAVVMLILWWVANRIYKSPFGR
                     TLRSIREDIDVSEAFGKNAFRYRMIAMVIGCFYAGIAGGLLVGYVSAFSPGGWTAGET
                     FVVWAALLIGGRGNNWGAVLGALLVPVLFQEVTRFLPQISSNAELIPALRNIIVGSLL
                     ILVLWVRPQGVIPERKARFFDLPLARPPRRQEETNVASN"
     gene            760..1527
                     /locus_tag="VGT44_03530"
     CDS             760..1527
                     /locus_tag="VGT44_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2579902.1"
                     /translation="MSQATDILLVEGVTHAFGGVKAVDNCSLSVKAGAITALIGPNGA
                     GKSTLVNIVSGFYRLQHGRVVFNGHETGGLPSYRVAHRGLIRTFQISRDYGGMTVLEN
                     MMVPPQSQAGESLFNALFRPRYVAKDEKRHLEKAYKLLDTFGLYTKRDDYARNLSGGQ
                     KRLLEMARALMADPKLLLLDEPMAGVNPALAEQLAAHILELSTTGVTFLMIEHNLGIV
                     DQICEQVIVMANGSTLATGSMADLRAHPEVVEAYLGR"
     gene            1532..>1922
                     /locus_tag="VGT44_03535"
     CDS             1532..>1922
                     /locus_tag="VGT44_03535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2579903.1"
                     /translation="MAIFQIERMTGGYSRLPVVQEVSISAEKGQVVSIVGPNGAGKST
                     LLKLASGTLHSMSGQVHVKDRNLSGLPANRVARLGLAYVPQERNVFPTLTVHENLEMG
                     GFILSGSLKERIDHLFDLFPDLRTAARL"
BASE COUNT          364 a          510 c          612 g          436 t
ORIGIN      
        1 tgctggccgg tggagtcgtg gcggcgctgt ttggggtgct ggtcggcttc attgccttga
       61 agcggctgcg cagcgattat atggcaatcg tcacagtggc tgtgggagcc atcgtctatg
      121 acttcatcgg caactacacg cccttgttca atggatggga tggcctgggt ggtgttccga
      181 acccgctcta cacgacggtc aacctcgatt ttcagaccta tcaatggttc tacgtcggtc
      241 tgaccgcagt ggtgatgttg atcctctggt gggtggccaa ccgtatctac aaatctccct
      301 ttgggcgcac gctccgctct atccgcgagg atatcgatgt gtcggaggcg ttcgggaaaa
      361 atgcgtttcg ctaccgcatg atcgccatgg tgatcggctg cttctacgcc ggcatcgcgg
      421 gtgggctgct ggtgggctat gtcagcgcct tcagccccgg cggttggact gctggagaga
      481 cgtttgtcgt ctgggctgcg ctgctgatcg gcggacgcgg caataactgg ggagctgtgc
      541 tgggcgcgct gctggtgccg gtgctgttcc aggaggtaac gcgcttcctg cctcaaatca
      601 gctccaacgc tgaactgatc ccggccctgc gcaatatcat cgtcgggtcg ctgttgatcc
      661 tggtgctgtg ggtccgaccg cagggtgtca tccctgagcg caaagcacgc ttttttgatc
      721 tgccgctagc acggccgccc agaaggcagg aggaaacgaa tgtcgcaagc aactgacata
      781 ctcctggttg aaggagtgac gcacgctttc ggtggcgtca aggcggtgga taattgcagc
      841 ctcagcgtga aagcgggcgc gattacagcc ctgatcggcc cgaatggagc tgggaagagc
      901 acgctggtca atatcgtttc aggtttctat cgcctgcaac acgggcgggt ggtgttcaac
      961 ggtcatgaaa ccggtggttt gccgtcctac cgtgttgctc acagaggtct gatacgcaca
     1021 tttcaaattt cgcgggatta cggcggcatg acggtgctgg aaaatatgat ggtgccgccg
     1081 caatcgcagg caggcgagtc gctcttcaat gccctgtttc gcccgcgtta cgttgcgaaa
     1141 gacgagaagc ggcaccttga aaaggcttac aagctgctcg ataccttcgg cctgtataca
     1201 aaacgcgatg actacgcgcg caacttgagc ggcggccaga agcgattgct ggagatggcg
     1261 cgggcgctga tggccgatcc gaagctgctg ctgctggacg agccgatggc cggcgtcaat
     1321 ccggccctgg ccgagcagtt ggccgcgcac atccttgagc tgagtacaac gggcgtcacg
     1381 tttctgatga tcgagcacaa tcttggtatc gtggatcaga tttgcgagca agtgattgtg
     1441 atggccaatg gaagtacgct cgccaccggt tcgatggctg atctgcgagc acatccagag
     1501 gttgtcgagg cctatctagg gaggtgattt cgtggcaata tttcagatcg agcgcatgac
     1561 cggcggctat agtcgtctgc cggtcgtgca ggaagtgtcg atttcggctg aaaagggcca
     1621 ggtagtttct atcgttggac cgaacggggc cgggaaatcg accctgctga aactggcatc
     1681 agggacgctg cactcgatga gcgggcaggt gcatgtgaaa gatcgcaacc tctcgggctt
     1741 acctgccaat cgtgtagcgc ggcttggact tgcctatgtg ccgcaggagc gcaatgtctt
     1801 ccccactctg acggtacacg aaaacctgga gatgggcggc tttatcctgt cgggtagttt
     1861 gaaggaacgt atcgatcacc tgtttgatct cttccccgac ctgcgcaccg cagcgcggct
     1921 ga
//
LOCUS       DASZAK010000208         5231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_40451, whole genome shotgun sequence.
ACCESSION   DASZAK010000208 DASZAK010000000
VERSION     DASZAK010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5231
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40451"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VGT44_03540"
     CDS             complement(<1..244)
                     /locus_tag="VGT44_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579904.1"
                     /translation="MDLSQWFSTQLSASAEGFLWAIEQVPPERRPATPPKPLGEWNVA
                     RHLFHMTYYEQLIALPSMKIWLNEPFTLTDEEYDEDA"
     gene            complement(279..1046)
                     /locus_tag="VGT44_03545"
     CDS             complement(279..1046)
                     /locus_tag="VGT44_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579905.1"
                     /translation="MQHIHTVSRPGFARLYRSPSITVARLTLESYLRSGWMWGEFVLV
                     LVFFAALFFPFMENVAYFYGTSAWDLSAIAVLGAVVMVRQSTSARTYVVLARLTSRAA
                     YSRGLILATAALRVPILLFFCALVLVAHRLTNPTPGTMVISALGLLPITILAATLTVT
                     FTSPIGTRRKRMLLLIWIAVVLFSLSPLILLPQIVVGALSFSRIPLAPIAVCYNVSVN
                     GSIDVNSLWGFPLIVTYIVGLAWLAGYWLEKKELLLY"
     gene            complement(1050..1985)
                     /locus_tag="VGT44_03550"
     CDS             complement(1050..1985)
                     /locus_tag="VGT44_03550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2579906.1"
                     /translation="MSQDAQPVIACTGLTKIYNNGVTALDNLTLAIQRGESFGLMGEN
                     GAGKSTLVRLLMGFLFPTRGELTVLGENEVRKAHRRIGYLHERPYVELRFTGRRYLTY
                     MAQLSGLWENGCRERVAGVLEQVDLGLAADEKIATYSKGMLQRLMIAQTLLTEPELLI
                     LDEPTNGLDPYSQWKMRQIIAALRKQGKTILLCSHYLAEVEMLCDTVGILQRGHLAQY
                     GAVADLVHTTEAVDILLVEGQDGDEIAQRLGLANYIIETDGSLLRIHASAQQVVLAAL
                     VEASIPIHTLHPVSSTLEEVYIKSTRPGVEAGMGV"
     gene            2157..>5231
                     /locus_tag="VGT44_03555"
     CDS             2157..>5231
                     /locus_tag="VGT44_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2579907.1"
                     /translation="MSLSTETEEKPVMTPARKAGQGRGRRGGRLPNIITLAGWRVRET
                     WRLLVITGLGSIAAVMLVCAVPLYSDVSMSAGLRGVIGASYQTSDVVVQGQSAVVSLD
                     FINKTTQQLNAEFQDKLGTYLKPPEFSVIGSSVSLMINHPTRCSGQETAPPYLSCDLI
                     QFISTSMQQTSPHLHLIQGRLPSDSTDGQSLDIALTDESAAAMHVTVGSLLHVTFTSS
                     ETPVKSINLPVTLHVVGIFTPPNGDDPFWHGNNYESVPRGNLGTAYTALASNVAVLSL
                     FDKAFARPALANTTLDAPLTLQWYYPLDPSRIVISNLDSILGSINQVQVDVSNNTSLN
                     QPPQLSQPTVVLPSDILQRYHDRIAVANIPVLSLLAFVLALALFFVSMMANFLVERQS
                     DSITILRSRGASRSQIFGSFMVQSVTLGLVALVAGPLLAVLLVFIVIQRALSPADQNV
                     LAVISGNPGAVAANLGLYALIAAGASVLAMALAIYRAMRFDILALRREMARQTSRPAW
                     QRINLDIVAIIITLVGFGVSYYITNSGVLDPQLRLLLLSPLTLLETVFVLIAYLLLFL
                     RLFQLLLRLGSWFASYGRGASSMLALAQMARSPRQSTRMTLLFSLATAFMIFTFIFIA
                     TQAQRVPAVSAYQAGADFSGTPTDSSLESVALDSETSMYRQVPGVTSATLGYQTGTIG
                     GGNILSISIELEAVDANSFAQTVGPNWTSQNSSQPLPALMSQLSQARRGAINGGVIPA
                     FVDSAAWANLHLSPGKTFELSFSTYGSLIFVAEGEINQIPTIDDNVSGSSSGDVPSGA
                     VLVDYRTLAPVYNTTFAQYGAGLSINYAWLRSRDDAASLASVRNALTTGCCLTLNPMF
                     DRRATITNLQNDPLSIDLLGLLTMGATTAMLLALAGSLIASWLNARSRVANFAVLRAL
                     GASPPQIAGILTWEQAIVYITSLLLGLLFGAILAALALPVMVFTSVPSTGAGSSASSS
                     AFFVQQNTPPIQIVIPVVLIYVLAALVLVCIIALALMIRIVSRPSVSQTLRLN"
BASE COUNT         1078 a         1625 c         1422 g         1106 t
ORIGIN      
        1 cggcatcctc atcgtattct tcatcagtca gcgtaaatgg ttcgttcagc cagattttca
       61 tgctgggcag cgcgatgagt tgttcatagt acgtcatatg gaacaggtga cgcgccacat
      121 tccactcgcc cagaggtttt ggcggagtag ccggacggcg ctcaggtgga acctgctcga
      181 ttgcccagag aaagccttcc gcgctagcag ataattgtgt gctaaaccac tgtgaaaggt
      241 ccatcgtttt aggtcctttt gctgcttcgc ctcaattgct aatacagcaa taattctttc
      301 ttttccagcc agtagcccgc caaccatgct agcccaacga tataggtgac gatcaacggg
      361 aagccccaca ggctgttcac atcgatacta ccattgacac tcacattgta gcagacagcg
      421 ataggggcga gtgggatgcg tgagaagctc agcgcaccaa cgacaatctg cggtaaaaga
      481 ataagcggac tgagcgagaa caacaccact gcgatccaga taagcaagag catacgcttg
      541 cggcgcgtgc cgataggcga agtgaatgta acagtgaggg tggcagccag gatggtgata
      601 ggcagcagtc ccagcgcgct aatcaccatt gtacccggcg ttggattggt gagacgatgc
      661 gccaccagca caagcgcgca gaagaagagc aagataggaa cacgcagcgc cgccgtcgcc
      721 aatatcaggc cgcgactata ggccgccctc gatgtgagcc gcgccagcac tacatacgtg
      781 cgcgcgctgg tggactgacg caccatgacc accgccccca gcacggcaat cgcgcttaag
      841 tcccaggccg acgttccgta aaaatacgcc acgttctcca taaaaggaaa gaagagagcg
      901 gcgaagaaga ccagcaccag cacaaattcg ccccacatcc agccactgcg caaatagctc
      961 tcaagcgtca ggcgcgcaac cgtgatggat ggggagcgat agaggcgagc aaagcctggg
     1021 cgagatacag tatgtatatg ttgcatatcc tataccccca tacctgcttc gacgcctggg
     1081 cgcgtactct tgatatacac ttcttcgagc gtcgagctga cgggatgcag cgtgtggatg
     1141 gggattgatg cttcgacgag cgccgccagc actacctgct gagcgctggc gtggatacgt
     1201 agcagactgc catccgtctc gatgatataa ttcgcgaggc caagacgctg cgcgatttca
     1261 tcgccatcct gcccctctac cagcaagatg tctaccgcct ctgtcgtatg caccaggtca
     1321 gcgaccgcgc catattgcgc caggtggccg cgctgcaaga tgccgaccgt atcgcacaac
     1381 atctccacct ccgccaggta atgcgaacag agcaggatgg tcttgccctg tttgcgcagc
     1441 gccgcgatga tctggcgcat cttccactgg ctgtacggat caagcccatt ggtcggctcg
     1501 tcaaggatca gcagctccgg ctcggtcagc agcgtctgcg cgatcatcaa gcgttgcagc
     1561 atacctttgg agtaggtagc gattttttcg tcggctgcca atcccaggtc aacctgttcc
     1621 aacacccccg cgacgcgctc gcggcagcca ttctcccaaa ggccagagag ctgcgccata
     1681 taggtcagat agcggcgacc cgtgaaacgc agttcgacat aggggcgttc gtgcaggtag
     1741 ccaatgcgcc ggtgcgcctt gcgtacctca ttttcgccaa gcacggtcaa ctctcccctc
     1801 gtcgggaaga gaaaccccat cagcaggcgc accaaagtcg atttgcccgc cccattctcg
     1861 cccatcaacc caaagctttc acctcgctga atggccaggg tcagattatc aagggcggtc
     1921 acgccattgt tgtatatttt agtcaggccg gtacaggcga tgacaggttg agcatcttga
     1981 gacaatcgtt cactcataat ggatgcaagt ataactgcaa tacttttagg atgcaagcgt
     2041 catttaagtt aggggtaggc gtgtcggagg gcgtaccctt gcggtcgccc tcggaaactc
     2101 gacggtcggg ctttgggaaa ccccgctccg aggcattatt tggaatcgag gatggtatgt
     2161 ccctttcgac agaaacagaa gaaaagcctg tcatgacgcc cgcgcgcaaa gcgggacagg
     2221 ggcgtgggcg acgtggaggg aggctgccga acatcatcac gctggccggg tggcgtgtgc
     2281 gcgagacatg gcggctgctt gtgattaccg gcctgggcag tatcgccgca gtgatgctgg
     2341 tctgtgctgt gccgctctac tccgacgtgt cgatgagcgc cgggctgcgc ggcgtaattg
     2401 gcgcgtcgta ccagacatcg gatgtggtgg tgcaggggca gagcgcggtg gtctcattgg
     2461 actttatcaa caaaacaaca cagcagttga atgcagagtt ccaggataaa ttaggaacat
     2521 atctgaaacc gcctgaattt tcggtgatag gttcttccgt gtcgctgatg atcaaccatc
     2581 ccacacgttg tagcgggcag gagactgctc caccgtatct gagctgcgac cttatacaat
     2641 tcatctcaac ttcgatgcag caaaccagcc cgcacctgca cctcatccag ggacgccttc
     2701 ccagcgatag cacagatggc cagagtctcg acatcgcgtt gacggacgag agcgcggcag
     2761 ccatgcatgt gactgttggc tcactactcc acgtcacttt cacttccagt gaaactccgg
     2821 tgaaaagtat caatctgccg gtcacactgc acgtcgttgg tatctttacc ccaccaaacg
     2881 gcgatgatcc cttctggcac ggcaacaact acgaaagcgt tcctcgcggc aatctaggaa
     2941 cagcctatac cgcgcttgcc tcgaacgtgg cggttctgtc gctattcgac aaggcattcg
     3001 cccggcccgc gttggcaaat acaacgttgg atgctcctct gacgctgcaa tggtactacc
     3061 cgcttgaccc atcacgcatc gtcatcagca acctggatag catcctgggc agcatcaacc
     3121 aggtccaggt ggatgtcagc aataacacgt cgctcaacca gcccccgcaa ttgtctcagc
     3181 ccaccgtcgt gctgcccagc gacatcttgc agcgctacca cgatcgtatc gccgtcgcga
     3241 atatccccgt cctgagcctg ctggcctttg tgctggccct ggccctcttc ttcgtcagca
     3301 tgatggccaa tttcctggtc gaacgccagt cggattcgat caccatcctg cgcagtcgcg
     3361 gagccagccg ctcccagatc ttcggctcct tcatggtgca aagtgttacg cttggcctgg
     3421 ttgccctggt cgcggggccg ctgctggcgg tcttactggt cttcattgtg atccagcgcg
     3481 cactctcccc ggcagaccag aacgtgctgg cggtcatttc aggcaaccct ggcgcggtgg
     3541 ctgccaacct gggactctac gcgctgatcg cggcgggggc atccgttctt gctatggcgc
     3601 tggcaattta tcgcgccatg cgcttcgata ttctggcttt gcggcgagaa atggcgcgcc
     3661 agacctcgcg gccagcctgg cagcgcatca acctggatat cgtcgctatc atcattacgc
     3721 tggtcggctt cggcgtctcc tattacatca ccaactcagg tgtgcttgat ccccaattgc
     3781 gcttgctcct gctctcgcca ctgacactgc ttgaaacagt cttcgtgctg atcgcctatc
     3841 tcctgctctt cctgcgactc ttccaattgc tcttacgcct ggggtcctgg ttcgcatcct
     3901 acggtcgcgg cgcatcgagt atgcttgccc tggcgcaaat ggcgcgctcg cctcgccaat
     3961 ccacgcgcat gactctgctt ttctccctgg cgacggcctt catgatcttc accttcatct
     4021 tcattgccac gcaggcccag cgcgtaccgg ctgtctcggc ctaccaggca ggcgcggatt
     4081 tcagcggcac acccacggat agcagcctgg aatcggtcgc gctagattct gaaaccagca
     4141 tgtatcgcca ggttcccggt gtgacatcgg ccactctggg gtaccagaca ggcacaattg
     4201 gcggtggcaa tatcctctcg atctccatcg aactggaagc cgtcgacgcc aactcgttcg
     4261 cgcagaccgt tggacccaac tggaccagcc agaactcatc acagccactg cccgcgttaa
     4321 tgtctcagct ctcccaggcg cgcaggggag caataaacgg tggagtgata cccgccttcg
     4381 tcgatagcgc ggcctgggcg aacctgcacc tgtcgccggg gaagaccttc gagcttagct
     4441 tctcgaccta cggctcgctc atatttgtgg cggagggcga aatcaatcaa attcccacca
     4501 ttgatgataa cgtcagcggc agcagcagcg gcgatgtgcc atcgggggcc gtgctggttg
     4561 attatcgcac actggccccg gtctacaata ccactttcgc gcaatatgga gcgggcctga
     4621 gcatcaacta tgcctggctg cgttcgcgtg acgatgcagc ctcgctcgcc agcgtgcgta
     4681 atgcgctcac caccggctgt tgccttaccc tcaacccgat gtttgaccgg cgcgctacca
     4741 tcaccaacct gcaaaatgac ccactctcga tcgatctgct gggtctcttg accatgggcg
     4801 cgaccactgc gatgctgctt gccctggcgg gaagcctgat cgcctcctgg ctgaacgcgc
     4861 gcagccgtgt cgcgaacttc gccgtcctgc gcgcgctggg cgcatcgccc ccgcagatcg
     4921 ccggaatact cacctgggaa caggccatcg tctacatcac ttcgctgttg ctcggcctgc
     4981 tcttcggcgc gatcctggcc gcgctggccc tgcctgtgat ggtcttcacc agcgtgcctt
     5041 ccaccggcgc gggcagcagc gccagcagca gcgcattctt tgtgcagcag aacactcccc
     5101 ccatccagat tgtgattccc gttgtgctca tctacgtgct ggccgcgctg gtactcgtct
     5161 gcatcattgc cctggcgctg atgatccgca tcgtctcgcg cccatccgtc agccaaacac
     5221 tacgcctgaa c
//
LOCUS       DASZAK010000209         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_573264, whole genome shotgun sequence.
ACCESSION   DASZAK010000209 DASZAK010000000
VERSION     DASZAK010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573264"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1184
                     /locus_tag="VGT44_03560"
     CDS             <1..1184
                     /locus_tag="VGT44_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579908.1"
                     /translation="NTLLFYNVVRLMSKKLVAIVLLAGTFALLSGGALARPTAHANYG
                     DLSVVGPPSLPAATVNAIFTRLGSPMSGTGAIVEQLSQQTQIDDAFALGVWWTETNDG
                     ASGVGLADRNPGSVRGSVGYPSAYDGYTIYPTYTDAIAYWFHMLRNLYVESRGLTTVY
                     AISHPYVGTSNSPLWADKVIALMLRYRGEAPPPTPMPTLMPSPTFSPYLPHHHPAAYL
                     GAGAPVPTTTVQWSGQPQGTLADSTQTGDSGSIATWLTVLAALLLALAIALWAVRYLP
                     GVKLYVYPLQIRAPDAPPPDAHKGHPYMSAGRPANPMRPLSLPVRPAVVPDVKYAPTS
                     LFVRLPETLGVGKIRRVMLLPERQEGGMRAVEEATDEEEATAKMRSVGLMARYGQRPQ
                     E"
     gene            1401..2333
                     /locus_tag="VGT44_03565"
     CDS             1401..2333
                     /locus_tag="VGT44_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEV2579909.1"
                     /translation="MATHHLDDTQPHAFWDNTLPPRVRIQPGDTVVFETLEASAGQVA
                     PGSPSEAVGHLNFDLIHPLTGPVYVEGAEPGDALAVDIVSIKHKGWGWNAVIPGFGLL
                     AEDFSAPYLHHYKLGEDCEFREDIHIPYEPFCGVMGVGPRESGRFTTIPPRENAGNID
                     IRHLTPGSRVFFPVLVPGALFSCGDCHSAQGDGEVNGTGIETKMTVTLNFSLIKGANI
                     PELRFITPPGKKLTVADEGGYYVTTAHGPDLYKDAQQAMRYMIDHLSTTYHMTREQAY
                     CLCGAAVDLKISEIVDAPNWIVSAYLPLSIFHAH"
     gene            complement(2406..>2723)
                     /locus_tag="VGT44_03570"
     CDS             complement(2406..>2723)
                     /locus_tag="VGT44_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579910.1"
                     /translation="TVQFGQSGQYGQSSPANYPLPYAQPAPIPVGNFTPSPSMPPQSF
                     NSMPTEAYNAPPVYTTQNGSTQNAQYGQSPNLPTCPNCGRPLVPNLPACGVCGMPLSA
                     MRR"
BASE COUNT          539 a          754 c          842 g          588 t
ORIGIN      
        1 gtaatactct cctattttac aatgttgtga ggctaatgag caaaaagtta gtggcaatcg
       61 tcctgcttgc tgggacattc gcgctgctga gcggtggcgc gcttgcacgc ccgacggcac
      121 acgctaatta tggcgatctg agcgtagttg gaccgccgtc gctgcccgcc gcgaccgtca
      181 acgcaatatt cacgcgcctg ggcagcccga tgagcggtac cggcgcaatc gtcgagcagt
      241 tgtcgcagca gacgcagatc gatgatgctt ttgccctggg cgtgtggtgg acggagacga
      301 acgatggcgc gtcgggcgtc gggctggccg accgcaatcc gggcagtgtg cgcggcagcg
      361 ttgggtatcc ctcagcttat gacggctaca ccatctatcc aacttatacg gatgccatcg
      421 cgtactggtt tcatatgttg agaaatttgt acgtggagag tcgcggactg acgaccgtct
      481 acgctatttc gcatccgtat gtcggcacgt cgaattcccc gctgtgggcc gataaagtga
      541 tcgcgttgat gctccgctac cggggcgaag cgccgccacc gacgccgatg ccgacgctca
      601 tgcccagccc gaccttttcg ccatacctgc cgcatcatca cccggcagct tacctgggcg
      661 cgggcgcgcc tgtgccaacc accaccgttc aatggagcgg tcagcctcaa gggacgctgg
      721 cggactcaac gcagacggga gattccggtt caatcgcgac atggctgact gttctcgcgg
      781 cgctgctgct ggcgctggct atcgcgctct gggctgtgag gtacttgccg ggggtgaaac
      841 tgtatgtgta tcccctacaa attcgcgcac cggatgcccc acccccggat gcccacaagg
      901 ggcatcccta catgtcggcg ggccgccccg ccaacccgat gaggccgttg agtttgccgg
      961 tgcggcctgc ggtggtgcct gatgtgaagt atgcgccgac aagtctgttt gtgcggttgc
     1021 cggagacact gggggtgggg aagatacgcc gcgtcatgct cttgccagag cggcaggagg
     1081 gtggaatgcg agcggtagaa gaggcgacgg acgaagaaga ggcgacggcg aaaatgcggt
     1141 cggtggggct gatggcgcga tatggtcaaa gaccacagga gtaagggatt cttgtagaac
     1201 agtgatacct tgctttttcc ttgtaatacg gtatcattat caataataca gttgcggcgc
     1261 aattgttacc ctcatactca gtaaccagca ggcgtatacc attgtaagtg ttgtccctgt
     1321 aggcgttacg tttcatctag cattattttc ctcctacccg tccgttgttt ctacctctcc
     1381 ggtagagaag gagtaattgc atggcaaccc atcatctcga cgatactcag cctcatgcgt
     1441 tctgggataa taccctgccc ccgcgtgttc gcatccagcc gggtgacaca gtggtctttg
     1501 aaacgctcga agcttccgcg ggacaggtcg cgccaggttc accttccgaa gcggtaggac
     1561 acctgaactt tgacctcatt catcccttga ccggaccggt gtatgtggag ggggcagaac
     1621 ccggtgacgc gctggcagtg gatatagtga gcatcaagca caaaggctgg ggctggaatg
     1681 ccgtgattcc cggctttggg ctactggcag aggatttctc cgcgccgtat ttgcatcatt
     1741 acaaactggg cgaggactgc gagtttcgcg aagatattca cattccttac gagccgttct
     1801 gcggcgtgat gggcgtcggc ccgcgcgagt cggggcgctt tacgacgatt ccgccgcgcg
     1861 agaatgctgg caacatcgac atacgccatc tcacccctgg ctcgcgcgtc ttcttcccgg
     1921 tgctggttcc gggcgcgctc ttctcctgcg gggattgcca ctcagcccag ggcgacggcg
     1981 aggtcaacgg cacgggtatc gaaactaaaa tgacggtcac gctgaatttt tcgctgataa
     2041 agggggcgaa tattccagaa ctgcgcttca tcacgccgcc cggcaaaaag ctcaccgtcg
     2101 cggacgaggg cggctattat gtgacgacgg cgcacggccc cgatctctac aaagacgcgc
     2161 agcaggctat gcgctacatg atcgatcacc tgtcaaccac ctaccatatg acgcgcgaac
     2221 aggcgtattg cctgtgcgga gcagcggtcg atctaaaaat cagcgaaatt gtagatgcac
     2281 ctaactggat tgtttcggcg tatctgccgc tgagcatctt tcacgcgcat tgacaagaca
     2341 caatgatgaa tgtccgagct tgatcgcgaa cattcatcgc gtccctacga ccggggggac
     2401 agcgattagc gccgcatcgc gctcagtggc attccgcaga cgccgcaggc gggcagattg
     2461 ggaacgaggg gtctgccgca attggggcag gtcggcaggt tgggcgattg gccgtactgg
     2521 gcattctgcg tgcttccatt ttgtgtggta tagacgggcg gggcgttata tgcctcggtt
     2581 ggcatggagt tgaacgactg agggggcatt gatggcgacg gcgtgaagtt ccctaccgga
     2641 atgggagcgg gttgggcata gggcaatggg tagttcgccg ggctgctttg cccgtattga
     2701 ccggattggc caaactgaac ggt
//
LOCUS       DASZAK010000210         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_5386, whole genome shotgun sequence.
ACCESSION   DASZAK010000210 DASZAK010000000
VERSION     DASZAK010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5386"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VGT44_03575"
     CDS             <1..568
                     /locus_tag="VGT44_03575"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEV2579911.1"
                     /translation="RQRAAFADAKPATHPHIAAWRQAYESFGAKPKKILCSVEALLSR
                     TLKGNDLPTINYIVDLYNAISIRHVLPVGGEDLDRLESDLVLTFATGSEPFDSVENGE
                     AVIAHPAPGEVVFADSAGVTCRCWNWRQCRRTQLTLDTRNAYFVLDCLAPYTIEELMC
                     AGEELMQYLRDTSPACAVTYELLGQHSS"
     gene            complement(569..1546)
                     /locus_tag="VGT44_03580"
     CDS             complement(569..1546)
                     /locus_tag="VGT44_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2579912.1"
                     /translation="MSEQQIQKVRVTRLAHVGLWANDVAAQTRFYRQMLGFNLRATEI
                     HPTDDEVEFEDANTFLSLGDEHHCLGLFNDIRGDAGGRSNGRSPVQNSRLHHIAFEVD
                     DDAALAALAERLNRTGIELMLKPRDGNPDMGDTLWFSDPDGNAIEISVMPDDSLTLPP
                     ASNEGRRARLRPYALQHLAIRTTRLEAMVDFYTEALGFDISDWLLRECVWMRCNTDHH
                     TLMLMQGKPDIDHIGFSISAGAELLAWADYLSRKQRPILWGPGRHGAGNDLFLRFADA
                     EGAHIELSAELQQYFDRDVTTPPRLWHSRATALNLWGVMPSWVREEVRV"
     gene            complement(1659..1907)
                     /locus_tag="VGT44_03585"
     CDS             complement(1659..1907)
                     /locus_tag="VGT44_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579913.1"
                     /translation="MNENDIKNCRGCRINMYWAFQHYEELATIREANKSVPRPETPPF
                     LIETSKRIDEGPTVGWLRNTIKKFGFKGAGKKVSTMAE"
     gene            complement(1966..>2385)
                     /locus_tag="VGT44_03590"
     CDS             complement(1966..>2385)
                     /locus_tag="VGT44_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2579914.1"
                     /translation="SKTRIVLLADSNDEKLLFDALRIGVHGYLPKTGSIDDLLKALRA
                     VQSGERVLGEPHAVTQVVNEFHRLAKEENRLQWGLSMTEVEVVRLASQGCTNKEIGTQ
                     LYWSEVQVKRKMQDIYRKLKVNDRAQAVAEAIHHSLI"
BASE COUNT          493 a          709 c          642 g          541 t
ORIGIN      
        1 gcggcagcgc gctgccttcg cggacgccaa accggccacg cacccgcata ttgcggcctg
       61 gcgccaggca tacgagagct ttggcgcgaa acccaaaaaa atcctctgtt ctgtagaagc
      121 cttgctcagc cggacgctca aagggaatga cctgccaacc atcaactaca ttgtagacct
      181 gtacaatgcg atcagcatca ggcatgtcct gcctgttggt ggggaagacc tggaccgcct
      241 ggaaagcgac cttgttctga cattcgcaac gggcagcgag ccgttcgatt cggtggaaaa
      301 tggtgaagcg gtaatagccc atcctgcgcc tggcgaggtc gtctttgcgg atagcgcggg
      361 cgtcacgtgc cgttgctgga actggaggca gtgccggcgc acccagctta ccctggacac
      421 gcgcaacgcc tactttgtgc tggattgcct ggcgccctac acgattgaag agttgatgtg
      481 cgcaggggaa gaattaatgc agtatttgcg agacacttcc cccgcttgcg ccgtgaccta
      541 cgaactgtta gggcaacatt cgtcgtaatt acactcgtac ctcttcgcgc acccacgagg
      601 gcatcacacc ccacagattg agcgccgtcg cgcgcgaatg ccacaggcgc ggcggcgtgg
      661 tgacatcccg atcgaagtac tgttgcagtt cggcggaaag ctcgatatgt gcgccctcgg
      721 catccgcgaa acgcaggaaa agatcgttgc ccgcgccgtg ccgacccggt ccccacagga
      781 tggggcgctg cttgcggctc aggtaatcgg cccacgcaag tagttctgcc ccggcagaga
      841 tgctgaagcc gatatgatcg atatccggct tgccctgcat gagcatcagc gtgtgatggt
      901 cggtattgca gcgcatccag acgcactcgc gcagcagcca atcggagata tcgaagccca
      961 gggcctcggt atagaaatcg accatcgctt ccagccgcgt cgtgcgaatc gccaggtgct
     1021 gcaaggcgta agggcgcagg cgcgcgcgcc gcccttcgtt gctggcaggc ggaagcgtca
     1081 gcgaatcatc gggcatgacc gatatttcga tggcgttgcc gtcggggtcg ctgaaccaca
     1141 gcgtatcgcc catatcggga ttgccgtcgc gcggcttcaa catcaattcg atgccagtgc
     1201 gattgagacg ctccgccagc gccgccagcg ccgcgtcgtc gtctacctca aacgcgatat
     1261 gatgcagacg cgaattttga accggcgagc gaccattgct cctgccaccc gcatcaccgc
     1321 gaatatcgtt gaatagcccc aggcagtgat gttcgtcacc gagcgagaga aacgtgttgg
     1381 cgtcctcgaa ttcaacctca tcgtcggtcg gatgtatctc cgtcgcgcgc aggttgaagc
     1441 cgagcatctg cctgtaaaag cgcgtctgcg ccgctacatc gttcgcccac aaccccacgt
     1501 gcgccaggcg cgtgacccgt accttctgta tctgctgctc gctcatgttg acctcctggt
     1561 aaagacacta tctgcatgta ttaacgtgta tcatacaggc ggtgagccag gcggtcaatc
     1621 aagctacctg aaagtcatag gtagccctga tacaacattc actctgccat tgtagaaacc
     1681 ttttttcctg cgcctttaaa gccgaacttc ttaatcgtgt tgcgcagcca accaacagtg
     1741 ggtccttcat cgattcgttt gctggtttcg atcaagaaag gaggtgtctc aggccggggc
     1801 accgatttgt tcgcttcgcg tattgtcgcc agctcctcgt aatgctggaa agcccagtac
     1861 atgttaatac ggcatcctct gcaatttttg atgtcgttct cattcattgt tccgcattgt
     1921 ggacactcta tcatgaccgt ccccttccta atcgttctct cgaagttaga tcaggctatg
     1981 gtgtatggct tcggcgactg cctgcgctcg atcattgacc ttcagtttcc ggtaaatatc
     2041 ctgcatctta cgtttgacct ggacctcgct ccagtacaat tgcgttccta tctctttatt
     2101 cgtgcatccc tgcgatgcca gccgcacgac ctccacctct gtcatgctga gtccccattg
     2161 taggcggttc tcctccttgg ccagccgatg aaactcgttc actacttgcg tcacagcatg
     2221 tggttcaccg agaacgcgct cgccgctttg cacagcccgt agggccttta gcaaatcatc
     2281 aatggaccct gtttttggca gatagccatg gacgccgata cgtagagcat cgaaaagaag
     2341 tttttcatca ttggaatcgg cgagtaacac gatacgtgtt ttgga
//
LOCUS       DASZAK010000211         2868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_360656, whole genome shotgun sequence.
ACCESSION   DASZAK010000211 DASZAK010000000
VERSION     DASZAK010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360656"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VGT44_03595"
     CDS             complement(<1..1082)
                     /locus_tag="VGT44_03595"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEV2579915.1"
                     /translation="MTIARAASDFQIPAFDGQAWLELFIMIVALILCGTASAAETALT
                     SISRIKLKNMVEEGDQKAIQIEKLISQPNTFLSTILVVNSVAVIVASSMATVLGLRFS
                     QTFGELLATVFSSLIVLIFCEITPKTAAVQNPLRVARAMVNPVRGAAWLLHPLVASLS
                     AITNFLVRIMGGQMIHRGPFVTEEELRLLVTVGEEEGVLEEEETEMIHSIFEFADTTV
                     REVMIPRIDMVTLESDATVDQAVDLALQGGFSRIPVFEETIDNVIGILYTKDMLRQLR
                     EGHNQLPIRELVRPAYFVPETKKLDDLLREIRQKRTHLAIVIDEYGSVSGLVTIEDLM
                     EEIVGDIRDEYDIEENLYEKISPDEY"
     gene            complement(1128..2582)
                     /gene="glmU"
                     /locus_tag="VGT44_03600"
     CDS             complement(1128..2582)
                     /gene="glmU"
                     /locus_tag="VGT44_03600"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV2579916.1"
                     /translation="MNTYAPVVLAAGMGTRMRSTVPKVLHPLAGLPLLAHVLNALDAL
                     PASPLFESYHATLQSHLPVVVVGYASEQIEQVFGERCVYTLQREQVGTGDAVLAARDA
                     VDALDPLPQTVLVCYGDTPLISGDALASVLAEHLARQATLTFLTAITERPSDFGRVVR
                     YADGRVRAIVERKQASEEQKRIKEVNSGVYCFDRQRLWQSLATLPRNGAGEYYLTDLV
                     GIASAQGRVVATVQGAFDETIGINDRVQLAQAEMLLRRRIVEQHMYNGVTILDPATTY
                     IDAGVEIGQDTVILPGTFITGQTRIGSACRIGPGTSIDACALGDGCVVRNSVLEEAVL
                     EDGVSIGPFSHLRKGAHLARNVRMGNFGEVKNSYIGPGTDMHHFSYVGDATVGQGVNI
                     GAGTITCNYDGVRKNHTTIGDGASIGSDTMLVAPVTIGEHATTGAGAVVNRDVPPWAL
                     VAGVPARLLRMKQPEQTEQVEQPTQATDGLRAKE"
     gene            complement(2709..2781)
                     /locus_tag="VGT44_03605"
     tRNA            complement(2709..2781)
                     /locus_tag="VGT44_03605"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2747..2749),aa:Gln,seq:ttg)
BASE COUNT          575 a          868 c          793 g          632 t
ORIGIN      
        1 atgtattcgt cggggctgat cttctcgtac agattttctt cgatgtcgta ttcgtcgcga
       61 atatcgccca cgatctcttc catcaaatct tcgatggtca caagaccgga gaccgagccg
      121 tattcgtcaa tgactatggc caggtgcgta cgcttctggc ggatctcgcg cagcaggtca
      181 tcgagtttct tcgtctcagg cacgaagtag gccgggcgta ccagttcgcg gatgggcagc
      241 tggttatgcc cctcgcgtag ctgtctgagc atatctttcg tgtacaggat gccgatgacg
      301 ttatcgatcg tttcctcaaa gacagggatg cgcgaaaaac cgccctgcaa cgccaggtct
      361 accgcctggt ctacggttgc gtcgctttcc agcgtcacca tgtcgatacg cgggatcatc
      421 acctcgcgca ccgtcgtgtc ggcgaactcg aagatgctgt ggatcatctc ggtctcttcc
      481 tcttcgagga caccctcttc ttcgcccacc gtcaccagca gtcgtagttc ttcctctgtc
      541 acgaaggggc cgcgatggat catctggccg cccatgatgc gcaccaggaa gttcgtgatg
      601 gcgctcaatg atgccaccag tgggtgcagc agccatgccg ccccgcgcac cgggttgacc
      661 atagcgcgcg ccacgcgcag tgggttctgt acagctgccg tcttgggcgt gatctcgcag
      721 aagatcagca cgatcagcga gctaaagacg gtggccagca gctcgccgaa ggtttgagag
      781 aagcgcagcc ccagcaccgt tgccatgctg gacgctacaa tcaccgcgac gctgttgacg
      841 acgagaatag tggaaagaaa ggtattcggc tgggagatga gcttttcgat ctggatcgcc
      901 ttctgatccc cttcttccac catgtttttg agcttgatgc ggctgatcga ggtaagcgcg
      961 gtttcggcgg cagaggcagt gccgcagagg atcaatgcca caatcataat gaagagttcc
     1021 agccatgcct gtccatcaaa cgctggaatc tgaaaatcgc tcgcggctct ggctatcgtc
     1081 attaccgcta ttatagttcc tgatccaggg ccatccattt tgacccatta ctcctttgcc
     1141 cgcaagccat ccgtggcctg tgtgggctgt tctacttgtt ctgtttgctc cggctgcttc
     1201 atacgcaaca agcgcgccgg tacgcctgcc acgagcgccc agggcggcac atcacggttc
     1261 acaaccgcgc ccgcgccggt cgtcgcatgt tcgccgatgg taacgggggc tactaacatt
     1321 gtatcacttc caatagaagc cccatcgcca atggtcgtat gattcttgcg caccccatcg
     1381 tagttgcagg taatcgtccc ggccccgatg ttcacgccct ggccgacggt ggcatcgccg
     1441 acgtagctga aatggtgcat atcggttccc gggccgatgt acgagttctt gacctcgccg
     1501 aaattgccca tacgcacgtt gcgcgcgaga tgcgcccctt tccgcaggtg actgaacggg
     1561 ccgatactga ctccatcttc taatacggct tcctccagca ccgagttgcg cacgacgcag
     1621 ccatcgccca gcgcgcacgc atcgatgctc gttcctggcc cgatgcggca ggctgaaccg
     1681 atgcgcgtct ggccggtaat gaaagttccc ggcagaatga cggtatcctg gccgatctct
     1741 accccggcat cgatgtaggt tgtagccggg tcaaggatgg tcacaccgtt gtacatatgc
     1801 tgttcaacga tgcggcggcg caataacatc tctgcctgcg ccagttgcac gcgatcattg
     1861 atgccgatgg tttcatcgaa cgcgccctgc acggtcgcca cgacacggcc ctgcgcgctg
     1921 gctataccga ccaggtcggt caggtagtat tcgcccgcgc cgttccttgg cagcgttgcg
     1981 agtgactgcc agagcctctg ccggtcaaaa caatacacgc ccgaattgac ctctttgatg
     2041 cgcttctgct cctcgctggc ttgctttctt tcgacgattg cgcgcacccg cccgtctgca
     2101 tagcgcacaa cgcgcccaaa atcggagggc cgctcggtaa tagcggtgag aaaggtaagc
     2161 gttgcctgtc gcgcgagatg ttcggccagc acgctcgcca gcgcgtcgcc actgatgagc
     2221 ggggtatcgc cgtagcacac cagcacggtt tgtggcagcg ggtcaagcgc gtcaacggca
     2281 tccctggccg ccagcaccgc gtctccggtg ccaacctgct cgcgttgcag tgtgtagacg
     2341 cagcgctcgc caaaaacctg ttcgatttgt tccgatgcgt agccaacgac aacgaccggc
     2401 aggtggctct gaagcgtcgc gtggtatgac tcaaaaaggg gggaagcggg aagcgcgtcg
     2461 agggcattga ggacatgcgc caggagtggc aaccccgcca gcgggtgcaa gacctttggc
     2521 acggttgaac gcatccgggt ccccatacct gccgctaaca ccacaggcgc ataagtgttc
     2581 atagcaacta atatatacct ttttctgatg tctacagctt cggggatgga atgtttaaag
     2641 ccaggactca gcgtatacga tgcatatatc ttgttccctg ccagccatcc cacttcatcg
     2701 caaaaaagct cgggcgcctg gattcgaacc agggaaatgt cggttccaaa gaccgatgcc
     2761 ttaccgcttg gctacgcccg atcactattg gctcacagca tcgtaagcac gttgcgatta
     2821 taacatgatg taaaagtatt ggcaaggcca gcacctcgtt attctcca
//
LOCUS       DASZAK010000212         6096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_395903, whole genome shotgun sequence.
ACCESSION   DASZAK010000212 DASZAK010000000
VERSION     DASZAK010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395903"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VGT44_03610"
     CDS             <1..1059
                     /locus_tag="VGT44_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2579917.1"
                     /translation="LNQLLVEMDGFDTHQAVVVIAATNRPDGLDIALLRPGRFDRRVT
                     VDRPDWNGRLAILKIHSRDVPMGPDVDLVAMARATPGMVGADLANLVNEAALLAARRN
                     QDYVTQRCFEEALDKIILGAERPLVLTEEDINVVAYHEGGHALAALMTPDADPITKVT
                     IVPRGQALGVTMATPLDDRYNYSKEYLLAQVVYALGGRAAEQVAIGHITTGAENDLQR
                     VTAIARQMVTRWGMSEKLGTISFSERSSPFAGVAEAGAPTDYSETTAELIDDEVDRIV
                     RQCYERAISIMTTYRVTLDHIAQALRKNETLDARQLRDILEETGARPAPDASPPAAVP
                     IEVAPPPPTEIYLPGENR"
     gene            complement(1141..1623)
                     /locus_tag="VGT44_03615"
     CDS             complement(1141..1623)
                     /locus_tag="VGT44_03615"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV2579918.1"
                     /translation="MELNDTTDYQRCFVCGQRNPFGLHLVFRVEGDSVVADFQPREEH
                     QGFPGVIHGGIVAAVLDESLGRTSLLGQSPEWTMTGRLEVRYRRYVPYGPLLRVRARL
                     ATERRRMLQATGVLTLSDDESVVLAEGVGTFLPLAPQVVDTIMQDYPELRLFMEHYDR
                     "
     gene            1873..2715
                     /locus_tag="VGT44_03620"
     CDS             1873..2715
                     /locus_tag="VGT44_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV2579919.1"
                     /translation="MFQRVAILGLGAVGGSIGLALRQAKAAQQVTGYDSGKGIAGRAH
                     KLGAIDAACTQVAEAVREAELIVLAAPIGEMRALLQQIASAAPKAAVVTDVASAKAQV
                     ISWAEEFLPSSQSFVGGHPINEKEAEGIDAAQVTLFQQRIYCLVPTRRTHPAALEKVS
                     TLVETLGGTVRFLEPAEHDSMIAATQQLPFLVSTALIQTVGSSPSWHDARLLAGDGLR
                     AITSLASGDPTSHRDSLLANSDQLVRCINDYMKHLGELRDRIASRDGSIDNLFRQAQK
                     MWDE"
     gene            2991..4100
                     /gene="ugpC"
                     /locus_tag="VGT44_03625"
     CDS             2991..4100
                     /gene="ugpC"
                     /locus_tag="VGT44_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV2579920.1"
                     /translation="MARVGFEHVYKNFNKVEVVHDINLDIKDKEFLVLVGPSGCGKST
                     CLRMVAGLEEPSAGEITIGERVVNGVDPKDRDIAMVFQNYALYPHMTVFDNMAFGLKL
                     RHVPATEIKKRVEDAAEILGLQLLLKRKPKELSGGQRQRVALGRALVRSAQVFLLDEP
                     LSNLDAKLRVQTRSEIIKIHQRVQTTFIYVTHDQTEAMTMGTRIAVLNAGLIQQIGPP
                     QELYDHPSNLFVAGFIGSPAMNFFTNVEVVNDGDATKVVVPGYGEVEVPPLYRQQARE
                     AASRKLTLGIRPEHFEDNATRPADALNGSTITATVEIVEHLGSELLVYMTGKEKQIVA
                     RLDPRSTARIGQTLNLHVQAEDMHLFDNETGEVLF"
     gene            4173..6035
                     /gene="malZ"
                     /locus_tag="VGT44_03630"
     CDS             4173..6035
                     /gene="malZ"
                     /locus_tag="VGT44_03630"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF008051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HEV2579921.1"
                     /translation="MSHVHWSQSVHHDGSPCYVMADGTITHGSTVRLRVRTGLDAPVK
                     SIFVRTNPDGEQHLAPLRLVAINAAVQWWEGEIQVKMTRNSYRFLLATAEGYWWLTAA
                     GIFQYTPTDATDFRILANFQAPSWVRDTVFYQIFPDRVADGDPSNNVRDHEYECYGKP
                     VIARAWGELPRSHRESGTVEFFGGDLQGIIQRLDYLQDLGINALYLTPIFTSPSNHKY
                     DIVDYKHVDAHFGGDQALIALRNALDERDMRLMLDLVPNHCSADHPWFLAAQADPNAP
                     SAEFFTFHKRPDEYEMWMGVRSLPKLNYRSLRLREEMYAGEDSVARHWLKPPFRIDGW
                     RVDVANMLARQGESQLEHKVGRGLHRAVKHTSPNAYLIGENFYDGSPQLQGDELDATM
                     NYRGFNVPLQQWLVGFDASLAWRPQLHDPVLLPTPALAEQWRAFMAAIPWQVAAQQFN
                     LLGSHDTRRIQTVVGEDETLARLAALLLFTFPGTPSVYYGDEIGMAGGNDPDCRRCMI
                     WDEQAWNSGRREFYKQLIRLRRSSPALRHGGLQMLYAAGETLAFMREAPEERLIVVTR
                     RAADGLEALPVRHGGLPDGLVLREVISGAETAVEQGMLSLAGLPDVGMQLWRNA"
BASE COUNT         1278 a         1892 c         1773 g         1153 t
ORIGIN      
        1 ctcaaccagt tgctggtcga gatggacggc tttgacaccc accaggcagt cgtggtgatt
       61 gccgccacga accgccccga cgggctggat atcgccctgc tgcgtcctgg ccgcttcgac
      121 cgccgcgtca cggttgatcg acccgattgg aacgggcggc tggccatcct caagattcac
      181 tcccgtgacg tgccgatggg accggatgta gacctggtgg caatggcgcg cgccactccc
      241 ggcatggtgg gtgcggacct ggccaatctg gtcaacgagg cagctctgct ggccgcgcgc
      301 cgcaaccagg actatgtgac gcagcgctgc ttcgaggaag ctctcgacaa gattatcctt
      361 ggcgcggaac gcccgctggt cttgaccgag gaagacatca acgtggtcgc ctatcacgaa
      421 ggcggccacg ccctggccgc actgatgacg cccgatgccg acccgatcac aaaggtcacg
      481 attgtcccgc gcggccaggc gctcggcgtc acgatggcaa cgccgctcga cgaccgctat
      541 aactactcga aagaatacct gctggcacag gttgtctatg ctctgggcgg gcgcgccgcc
      601 gagcaggtgg cgattggcca catcaccacc ggcgcggaga acgacctgca gcgcgtcacc
      661 gccattgcgc gccagatggt cacgcgctgg ggcatgagcg aaaagctggg aaccatctcg
      721 ttcagcgagc gcagcagccc attcgcgggc gtcgccgagg caggcgcacc caccgattat
      781 agcgagacaa cggcggagtt gatcgacgac gaggttgacc gcattgtgcg ccagtgctac
      841 gaacgcgcca tctccatcat gaccacctat cgtgtcacgc tcgaccacat cgcgcaggcg
      901 ctgcgcaaga acgagaccct tgacgcgcgc cagttgcgcg acatcctgga ggaaaccggc
      961 gcgcgacccg cccccgacgc aagtccgccc gccgccgtgc caatcgaagt agcgcctcca
     1021 cccccaactg aaatctactt gcccggcgag aaccgataga ggacggcagt tggtgaggtt
     1081 ctagagggcg tacccttgcg gtcgccctct tatccactca gacaaatttc gcgccgacca
     1141 tcatctatcg taatgctcca tgaacaaccg caattcaggg taatcctgca taatcgtgtc
     1201 aacaacctgg ggggcaagtg gcaggaaggt ccccaccccc tccgcaagca cgacgctttc
     1261 atcatcgctc aaagtcagca cgcccgtcgc ctgcaacata cggcggcgtt cagtcgccag
     1321 gcgggcgcgc acacgcagca gtgggccata agggacgtag cgccggtagc gcacttccag
     1381 ccgaccggtc atcgtccact ccgggctctg acccagcagc gacgtgcgcc ccagtgactc
     1441 gtccaacacc gccgcgacga tgccgccatg gatcacgcca ggaaatccct ggtgttcctc
     1501 gcgcggctga aaatctgcta ccacactatc accctcaaca cgaaaaacca ggtgcagccc
     1561 aaatgggttg cgctgcccgc acacaaaaca gcgctgataa tccgtagtat cgttcagttc
     1621 cataagtctc ctgcccttga aatcttgtcg tttgcatgat tgtatcgtac caccgcatcc
     1681 agtggcaagc gcgcctccct ctctcccttc ccaaccaacc aactgtggtg gcctgtcaaa
     1741 gttcaatcgg catgtagggg cgcaattcat tgcgccctgg tagcggcgct tcacctggac
     1801 gagggcgcaa tgaattgcgc ccctacacgc cgattgaact ttgacatgcc actacgagga
     1861 ggagggtctg ctatgtttca gcgagtcgcc attctggggc tgggcgcggt cggcggttcg
     1921 atcgggctgg cactgcgcca ggcgaaggcg gcgcagcagg tgacgggtta cgacagcggc
     1981 aagggcatcg ccgggcgagc gcacaagctg ggcgctatcg acgcggcctg cacacaggtg
     2041 gcggaggctg tgcgcgaagc ggagctgatt gtgctggccg cgcccattgg agagatgcgc
     2101 gcgctgttgc aacaaatcgc gagcgcagct ccaaaggctg ccgtcgtcac cgatgtcgcc
     2161 agcgccaaag cccaggtaat tagctgggcc gaagagttcc tgccgtcttc gcagagtttt
     2221 gtcggcggac atcccatcaa cgagaaagaa gccgagggca tcgacgccgc gcaagtcacg
     2281 ctcttccagc agcgcatcta ctgcctggtt cccacgcgcc gcacacatcc ggcggctcta
     2341 gagaaagtct ccacgttggt cgagacgctc ggtggaacgg tgcgctttct agaacccgcc
     2401 gaacacgaca gcatgatcgc ggccacgcag cagctgccct ttctcgtctc aaccgcattg
     2461 atacaaacgg tcggcagcag tccttcctgg catgacgcca ggctgctcgc cggggatgga
     2521 cttcgcgcca tcacttctct ggcaagcggt gatccaacct cccaccgcga tagtttgctg
     2581 gccaacagcg accaacttgt gcgctgcatc aacgactata tgaaacacct gggcgagttg
     2641 cgcgaccgca tcgcttccag ggacggcagt atcgacaacc tgttcaggca ggcgcaaaag
     2701 atgtgggatg agtaaggcag ttctgcgcat ggcaaccgca aggggtgcca tgcgcatatg
     2761 aggaacttat acaaaaaaac actgcatttc cttcaaaact atggtatact tggatcagca
     2821 cgtatcgtct gatggcgctc taagcgcgtt ggcgaaagcg tgtcaagagg ccgcattgac
     2881 tcccgactct ttgtagttgc aatgcgcgca ccaagcaaca gctcgttatt gaaaaggtaa
     2941 caggggaaca ggacactcac ctgttacaag agcgaaaagg agttaccgta atggcacgtg
     3001 tcggttttga acacgtctac aaaaacttca acaaggttga agtggtccat gatatcaacc
     3061 tcgatatcaa ggacaaagag ttcctcgttc tggttggtcc ctctggttgt ggaaagagca
     3121 cctgtctgcg catggtcgcc gggcttgaag aaccctccgc cggcgaaatt accatcggtg
     3181 agcgcgtggt caacggcgtt gatcccaaag accgcgacat cgcgatggtg ttccagaact
     3241 acgcgctcta tccacatatg accgtcttcg acaacatggc attcggcctg aagctgcgcc
     3301 atgtgccggc gacggagatc aagaagcgcg tcgaggacgc cgctgaaatc ctgggcctgc
     3361 aactactgct caaacgcaag ccgaaggaac tctccggcgg tcagcgccag cgcgttgcgt
     3421 tgggccgcgc cctcgtgcgt tcggcgcagg tcttcctgct ggacgaacca ctttctaacc
     3481 tggacgcgaa attgcgcgtt cagacgcgct cggagatcat caagattcac cagcgcgtac
     3541 agaccacctt tatctatgtc acccatgacc agaccgaggc gatgacgatg ggaacgcgca
     3601 ttgccgtcct taacgccggg ctgatccagc aaatcggacc cccgcaggag ctgtacgatc
     3661 acccatccaa cctgttcgtc gccggtttca ttggctctcc ggccatgaac ttcttcacca
     3721 acgtcgaggt cgtcaacgac ggcgatgcga cgaaagtcgt cgtgcctgga tatggcgaag
     3781 tggaagttcc gccgctttac cgccagcagg ccagggaagc agcttcgaga aagctcacac
     3841 tcggcattcg cccggagcat ttcgaggata atgccactcg ccccgcggat gccctgaatg
     3901 gctctacgat cacagcgaca gttgagatcg tcgaacacct gggcagcgaa ctgctggtct
     3961 atatgaccgg caaggaaaaa cagatcgtgg cgcgtcttga tccgcgttcc accgcgcgca
     4021 tcggccagac actcaatctg cacgttcagg ccgaggacat gcatctcttc gataacgaaa
     4081 caggcgaggt gctattctaa tagcattatg cgccaatcaa aacggggatg gtcagcatat
     4141 ggccatcccc gtttgtatag aaaggttcag gcatgtctca cgttcactgg tcgcagagcg
     4201 ttcaccatga cgggtctccc tgctacgtga tggcggacgg gaccatcacc cacggctcga
     4261 ccgtcaggct ccgtgtgcgt actggcctgg atgcgccggt taaaagcata tttgttcgca
     4321 ccaaccccga cggcgagcag cacctggctc cgctgcggct cgtcgccata aacgctgccg
     4381 tgcagtggtg ggaaggggaa atccaggtca agatgacgag gaattcctac cgctttttgc
     4441 tcgcgactgc cgagggctac tggtggctga ctgctgctgg aatattccaa tacacgccga
     4501 cagacgcgac ggattttcgt attctcgcca atttccaggc cccgtcctgg gtgcgcgata
     4561 ccgtttttta ccagatattt cctgaccgcg tcgccgatgg cgacccctcc aacaatgtgc
     4621 gcgatcacga atatgaatgc tatggcaagc cggtcattgc gcgagcctgg ggcgagcttc
     4681 cacgctcgca tcgcgagtcg ggcacggtcg aattcttcgg tggcgacctg cagggaatta
     4741 tccagcgcct tgattacctg caagacctgg gcatcaacgc gctctatctc acgccgatct
     4801 tcacgtcacc cagcaatcac aaatacgaca tcgtggatta caagcacgtc gatgcccatt
     4861 ttggcggcga tcaggcgctg atcgccctgc gaaacgcgct ggatgagcgg gacatgcgcc
     4921 tcatgctcga cctggttccc aaccattgca gcgccgatca tccctggttt ctggctgccc
     4981 aggccgatcc caatgcgccc agcgccgaat ttttcacgtt ccataagcgt ccagatgagt
     5041 atgagatgtg gatgggggtg cgcagcctgc ctaaactcaa ctatcgcagt ctgcgcctgc
     5101 gcgaggaaat gtatgcaggc gaggattcgg tcgcgcgcca ctggctgaag cctcccttcc
     5161 gtatcgacgg ttggcgtgtg gacgtagcga atatgctggc gcgccagggc gagagccagc
     5221 tcgaacataa ggttggacgc ggtctgcacc gggcagtcaa acacacttct ccgaacgcct
     5281 atctcatcgg tgaaaatttc tacgatggct cgccgcagtt gcagggcgac gaacttgacg
     5341 cgacgatgaa ctaccggggg ttcaacgtgc cgctccagca gtggctggtt ggctttgatg
     5401 cgtcgctggc ctggagaccg caactgcatg acccggtact gctgccaaca cctgccctgg
     5461 ccgagcagtg gcgcgcgttt atggcggcta tcccctggca ggtcgctgcg cagcagttca
     5521 acttgctcgg cagccacgac acgcggcgca ttcaaacggt ggtcggcgaa gatgaaacac
     5581 ttgcacgcct ggccgccctg ctgcttttta cgttccccgg cacccccagt gtctactacg
     5641 gcgacgagat cggcatggcg ggcggcaatg accccgattg ccgccgctgc atgatctggg
     5701 atgagcaagc ctggaacagc gggcgacgcg agttctacaa gcagctgatc cgcctgcgcc
     5761 gctcatcgcc cgccctgcgt cacggcggtt tgcagatgct ctacgcggcg ggcgagacgc
     5821 tggccttcat gcgcgaagcg cccgaagagc gcctgattgt ggtaacgcgc cgcgcagctg
     5881 atggcctgga agcgctgccg gtacgccacg gcggcctgcc cgacggcctt gttttgcgcg
     5941 aagtgattag cggcgcggaa acggcggtag agcagggaat gctgtccctc gccggcctgc
     6001 cagacgtagg gatgcagttg tggcgcaatg catgagtctt gacattccgc taattgcgag
     6061 atatgctggt gtcagcaata cagttgaatg cgcaac
//
LOCUS       DASZAK010000213         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_325261, whole genome shotgun sequence.
ACCESSION   DASZAK010000213 DASZAK010000000
VERSION     DASZAK010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325261"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2135)
                     /locus_tag="VGT44_03635"
     CDS             complement(<1..2135)
                     /locus_tag="VGT44_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV2579922.1"
                     /translation="MPTLEGSIESIVFRNEENHYTVARFRLNDNGRLFRDDLTTIVGT
                     LPGVHVGELLTVEGEWEKDPRYGRQLHISSFVQRLPASKEGITRYLGSGLIKGIGPKK
                     AERIVAFFGEQTLAIIEQQPDRLSEVKGISVKDREQIIQSWTEQNEVKELHLFLQSHD
                     VSMTLATRIYKHYGQDSIKVIRENPYQLASDVNGIGFRTADEIAVKLGLPRDSIPRLS
                     TGLKYVLAQAANEDGHCFLPEHELLRRASEILQAAPDLLAEAMEQLKTEKDVFIEPPL
                     PPLTASSTTSEARAEEPPPDDSVAQEAMQRVYFGPFWHAEQGSARLMRKLMRTPSILP
                     AVSQQYWQIVFDFLAERKMVLTEKQREAVQMAYHKKVSILTGGPGTGKTTSLRALLMV
                     LRKRNVSVALAAPTGRAAKRLTEATGSIGFEAKTLHRLLEFAPHENSFARNEENALPY
                     QFVIVDEVSMVDILLFYHLLKALPSDAHLLLVGDADQLPPVGPGNVLRDLLRSEAIPT
                     VRLTELFRQAQQSQIVVNAHRINAGQMPSLKREQNTDFFFVHEDDPVHAQQLVLDFVQ
                     RRIPKHYQLNPLTDIQVLSPMYRGPVGVNSLNEELQARLNPDAEVALEWGGRVFRIGD
                     KVMQQRNDYDKDVVNGDVGWIRAINRENSTVKVEFLEEAGPLTASYEFHELDELTLAY
                     AVTVHKSQGSEYPAVILPLTREHYMLLQR"
     gene            2359..>3447
                     /locus_tag="VGT44_03640"
     CDS             2359..>3447
                     /locus_tag="VGT44_03640"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HEV2579923.1"
                     /translation="MKRVLDSGILRVVIALVLVAILIAGGWLSELNPIFLQIFVALIS
                     ASGAIVAAYISRSQAQKKRNGKVRSARVKGYSKTENELLKMYQSKLRGDPRIAHVKIL
                     DMNRLLEIKNIYVQVKIHQETRLTFEPDATFHDSAFRIESDPTRILKANRSRIESRAN
                     VALDAIGVIGKNKHCVIVGDPGTGKTTLLKYLTLKSIRAIKEQTSNQSTLPIFPIYIE
                     LNAFASSDHEKLLEYAASLWNKRYNIAASYDVIMPLIEHRLAEGEALILLDGLDETLM
                     GGTKEEAEKKYSRVVDAITNLSTSYTESSIVVTVRKAVYYQQKRRLDGFTEYEMQEFR
                     SEDIKQLVHNWFAATPDPQRPCNAYDLLD"
BASE COUNT          809 a          876 c          961 g          801 t
ORIGIN      
        1 ttgcgctgca gcagcatgta gtgttccctg gtcaggggca ggatcacggc ggggtattcg
       61 ctgccctggc ttttatgcac cgtcacggcg taggctagcg tcaactcgtc cagttcgtgg
      121 aattcgtagc tggctgtgag cggcccggcc tcttccagaa actcgacttt gacggtcgag
      181 ttttcgcggt tgatggcgcg aatccagccc acgtccccgt tgaccacatc cttgtcgtag
      241 tcgttgcgct gctgcatcac cttgtcgcca atacgaaaaa cgcgaccgcc ccattccaat
      301 gccacctccg cgtcgggatt gagccgcgcc tgcaactctt cattgaggct gttcaccccc
      361 accgggccgc gatacatggg cgaaagcacc tggatatcgg tcaggggatt gagttgatag
      421 tgcttgggga tgcggcgctg cacgaagtcc agtacgagct gctgggcgtg aacggggtca
      481 tcctcgtgta cgaagaagaa gtcggtgttt tgttcgcgtt tgagcgaggg catctgcccg
      541 gcgttgatgc ggtgcgcgtt gacgacgatc tggctctgct gcgcctggcg aaacagttcg
      601 gtcaggcgca ccgtcgggat ggcctcgctg cgcagtaaat cgcgcaggac gttgcccggc
      661 ccgacgggcg gcaactggtc ggcatcgccc accagcagca ggtgcgcgtc ggacggcagc
      721 gccttgagca ggtgatagaa gagcaggata tcgaccatcg agacttcgtc tacgatcacg
      781 aactggtacg gcagggcatt ctcttcgttg cgcgcgaacg agttttcgtg tggagcaaat
      841 tcaagcaggc gatgcagggt tttggcctcg aagccgattg agccggtcgc ctcggtgagg
      901 cgcttggcgg cgcgtcccgt cggagcagcc agggcgacac tgacgttgcg tttgcgcaag
      961 accatcagca gagcgcgcag cgaggtcgtt ttgcctgtgc caggtccgcc cgtcaggatg
     1021 ctgaccttct tgtgatatgc catctgcacc gcctcgcgct gcttctcggt caacaccatc
     1081 ttgcgctcgg ccaggaaatc gaagacaatc tgccagtact gctgactgac tgccggcagt
     1141 atgctgggcg tacgcatcaa cttacgcatc agacgcgccg agccttgttc cgcgtgccag
     1201 aatgggccaa agtagacgcg ctgcatagct tcctgggcaa ccgaatcgtc ggggggcggt
     1261 tcctccgccc tggcttcaga cgtggttgaa gacgccgtca aaggaggcaa gggcggctcg
     1321 ataaagacat ccttctcagt tttaagctgc tccatcgcct ccgccagcaa atcgggcgcg
     1381 gcctgcaaaa tctcggaggc gcggcgcagc agttcgtgtt cgggcaagaa gcaatgccca
     1441 tcctcgttgg cggcctgcgc cagcacatac ttcaagccgg tcgacaggcg agggatgctg
     1501 tcgcgcggca ggccaagctt gacggcgatc tcgtccgccg tgcgaaagcc gatgccgttg
     1561 acatcggagg cgagctgata ggggttttcg cgaatgacct tgatggagtc ctgcccataa
     1621 tgcttataaa tgcgcgtcgc cagggtcatt gagacatcgt gcgattgcag gaagaggtgc
     1681 agttccttga cttcgttctg ctccgtccat gattggataa tctgctcgcg gtctttgacg
     1741 ctgatgccct tgacctcgct gaggcggtcc ggttgctgct cgatgatggc cagtgtctgt
     1801 tcgccaaaaa aggccacgat gcgttcggcc tttttcggcc caatgccctt aatcaagcca
     1861 gaaccgaggt aacgggtgat gccctccttc gaggctggca ggcgctgcac aaagctggag
     1921 atgtgcaatt gcctgccgta gcgcgggtcc ttctcccatt caccctccac cgtcaacagt
     1981 tcgccgacat gcacgccggg cagcgtgccg acaatggtgg tcaggtcatc gcgaaacagg
     2041 cgaccgttat cgttgagccg gaaacgcgcc accgtatagt gattctcttc gttgcgaaag
     2101 acaatgctct caatcgagcc ttcaagggtt ggcatactga atcattccgt ttgaatgctg
     2161 gtaaagtggg aaactgccag gcttcatctt agcatatctg gcagattctc gtcgagcgag
     2221 ctacactacg gaacagcaaa cgaaacatag tgtattatgg ccacaagtgt tatctaaggt
     2281 catgatctca gttgttctag atagaggcta atcgatacat tattttgtag tacatatgca
     2341 caatcggaaa actcaagcat gaaacgagtt cttgattcag gaatattacg ggtagtaatt
     2401 gcgctggtat tagtagcaat attaatagca ggcggatggc tcagtgaact caaccctatt
     2461 tttctccaga tctttgtggc gctaatttct gccagtggcg ctatcgtagc agcatacatt
     2521 tcgagatctc aagctcaaaa aaaaagaaac ggcaaggtga gaagtgcaag agtgaaaggc
     2581 tactctaaaa ctgaaaatga actccttaaa atgtatcaaa gtaaattgcg gggtgatccg
     2641 cgtatagctc atgtgaaaat cctggatatg aaccggcttc tggaaatcaa gaatatttac
     2701 gttcaagtaa aaattcacca ggagacaaga ctgacctttg aaccggacgc gacttttcat
     2761 gatagtgcgt ttcggattga gagcgatcca acgcggatat tgaaagcaaa tagatcgaga
     2821 attgaaagca gagctaacgt tgctctggat gctatcggag tcattgggaa aaacaaacac
     2881 tgtgtaatcg ttggcgatcc gggaactggc aagactacac tgctcaagta tctgacttta
     2941 aaatcgatca gggcgatcaa ggaacaaaca tctaatcaat ccacccttcc gatctttccc
     3001 atctatattg agttgaatgc ttttgccagc tcggaccatg agaaattgct agagtatgct
     3061 gcttctctat ggaataaacg ttataacata gctgcctcgt atgatgtgat catgccgctt
     3121 atagagcatc ggttggcaga gggggaagca ttgattcttt tagatggcct ggatgagact
     3181 ctcatgggcg gcacgaaaga ggaagctgag aaaaaatatt cgcgcgtagt agacgcaatt
     3241 acaaatttat caacgagcta tacagaatct tccatcgttg tcacggtgcg caaagcagtt
     3301 tattaccagc agaaaagaag attagacggt ttcacagagt atgaaatgca ggaatttcgc
     3361 tcagaagata tcaaacaatt ggtgcataac tggtttgctg ctactcctga tccccaaaga
     3421 ccttgcaatg cgtatgattt gttagat
//
LOCUS       DASZAK010000214         2804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_395948, whole genome shotgun sequence.
ACCESSION   DASZAK010000214 DASZAK010000000
VERSION     DASZAK010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395948"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2533
                     /locus_tag="VGT44_03645"
     CDS             <1..2533
                     /locus_tag="VGT44_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV2579924.1"
                     /translation="PSAYKIIGTPRPKVDAYAKVTGRALYADDIHLPRMAYGCLLRSP
                     HPHARILLIDTSRALELPGVLAIITGEDLPRKYGILPSSQDEYALAVDKVRYVGDPVA
                     AVAALDPDILDEALKLIRVEYEILPALMSIDEALAHPETKINDEARIGNIHKAVSYEF
                     GEVEAGFSEADYVREDWFYYEGNNHAPMEEHACVANWEPNPADPVGGKLTLWSSTQTP
                     HYVHREVSKVLDLPRSHVRVIAPHVGGGFGGKSDPFSHEICACELSRRVGRPVKITCT
                     REEVFLNHRGRHPVKMWIKTGVKRDGTLTAMHFRSFLDGGAYGSYGIATTYYTGALQT
                     VTYTLPAYKFEGMRLFTNKPPCGPKRGHGTTQPRYAVEAHLDKIAHDLGIDPVELRRR
                     NLIKPYTRTINGLRITSCALGECLDAVTERSHWHEFHAPSLQEKSAANGNVPASKSAV
                     KRGMGIAASSYICGAGKPIYWNNLPHSAVQIRLDRGGGVTVYCGATDIGQGSTSILAY
                     IVAEELGIQPEAIHLETADTTLTPVDLGSYSSRVTFMAGNAAIAAARKLKDQLLEVAA
                     DHLHAPVEELQFDGGVIYNEHDPGIALPFVKVIELAEARFGALVSTGSYAPPEGIHGE
                     YKGSGVGPSPAYSYSACVAQVAVDTETGEVRVEKLWMAHDVGQSINPLLVAGQVEGGA
                     YMGYGEAVMEQQVFRKGRHKIPSLLDYKLPTTLDTPDIETILIEVPDQEGPYGGKEAG
                     QGPLNPTIPAIANAVFDAIGVSIDEVPITPEKVLKALKAEATTTRAAARPQVPPVKAP
                     SPWPTRLIKWQPGAQDGASQAGEQGNGHKPVQNAGTFATATGEIS"
     gene            2533..>2804
                     /locus_tag="VGT44_03650"
     CDS             2533..>2804
                     /locus_tag="VGT44_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV2579925.1"
                     /translation="MLRLPPFRYLAPRTIEQTVQMLAQEGEQAMLVAGGTDLYPNMKR
                     RQFTPPVLIGLRAIAALKLIDGSPEQGMRIGAGVALSTLASNPLFQR"
BASE COUNT          577 a          858 c          821 g          548 t
ORIGIN      
        1 tccttcagcg tataaaataa ttggcacgcc tcgcccgaag gttgacgcat acgctaaagt
       61 gaccgggcgc gccctctacg ctgatgacat tcatctccca cgcatggcct atggatgtct
      121 gctgcgctcg ccccacccac acgctcgtat tctcctcatt gatacgagcc gcgctctgga
      181 gttgccaggc gttctggcta ttattacggg cgaggatctg ccgcgcaaat acggtatttt
      241 gccctccagc caggacgaat atgcgctggc ggttgacaaa gtgcgctacg tcggcgatcc
      301 tgttgccgct gttgctgcgc tcgatcctga catcctcgat gaggctctca agctcattcg
      361 tgtcgagtac gaaatcctgc cggcactgat gagtattgac gaggcactgg cacatcccga
      421 aacaaagatt aatgatgagg cgcgcatcgg caacatccac aaggccgttt cctacgagtt
      481 tggtgaggtg gaggcaggct ttagcgaggc cgactatgtg cgcgaagact ggttctacta
      541 cgagggcaac aaccatgctc cgatggagga gcatgcctgc gtggccaact gggagcctaa
      601 tcccgccgat ccggttggcg gcaagctcac cctctggtcc tcaacgcaga cccctcacta
      661 tgtccatcgt gaggtgagca aggtgctgga cctgccccgc tcgcacgtgc gcgtgatcgc
      721 tccccacgtg ggcggcggct ttggtgggaa gagcgacccg ttctcccatg agatctgcgc
      781 ctgcgaactg tcgcgccgcg ttggtaggcc ggtcaagatc acctgcaccc gcgaagaggt
      841 ctttctgaac caccggggac gccatcccgt gaaaatgtgg atcaagaccg gcgtgaagcg
      901 ggacggtacg ctgacagcca tgcattttcg ctcctttctg gatggcggcg cgtacggctc
      961 ctatggcatt gcaacaactt attacaccgg cgccctgcag accgttacct atactctgcc
     1021 cgcctacaaa ttcgagggca tgcgcctgtt taccaataag cctccctgcg gtcccaagcg
     1081 cggccatggc acaacgcagc cgcgctacgc ggtcgaggcg caccttgata aaattgccca
     1141 tgacctgggc attgatcctg tggaactgcg caggcgcaac ctgatcaaac catatacgcg
     1201 gacgatcaat ggcctgcgta tcaccagttg cgcgcttggc gagtgcctgg acgctgttac
     1261 cgaacgctcc cattggcacg agttccacgc tccttctctt caagagaaga gtgcggccaa
     1321 tggcaatgtt ccagccagta aaagcgctgt caagcgcggc atgggtattg ccgcctcatc
     1381 ctatatctgt ggcgcgggca agccgatcta ctggaacaac ctgcctcact cagccgtgca
     1441 gattcggctg gaccgtgggg gcggcgtcac ggtctactgc ggcgcgaccg acattggcca
     1501 gggctcaacc tccatcctgg cctacatcgt ggcggaggag ctggggattc agccggaagc
     1561 gattcacctg gaaacggcgg atacgacgtt gacccccgtc gatcttggct cgtattccag
     1621 ccgcgtgacc ttcatggccg gaaacgccgc tattgcggcg gcccgcaagc tgaaagacca
     1681 gctccttgag gtcgctgctg accatctgca tgcgccggtc gaggagttac aatttgatgg
     1741 cggcgtcatt tacaacgagc acgatcctgg catcgccctg ccgttcgtaa aagtgataga
     1801 gctggcagag gcccgtttcg gcgcgctggt ctcaacaggt tcatatgccc ctcctgaagg
     1861 cattcatgga gaatacaaag ggtcaggcgt cggaccttcc ccggcgtact cctactcggc
     1921 ctgcgtggcc caggtagcgg tggataccga gacgggggaa gtgcgcgttg agaaactctg
     1981 gatggcgcac gacgtggggc aatccatcaa tcccttgctg gtggccggtc aggtcgaggg
     2041 cggcgcgtat atgggctatg gcgaggcggt catggagcag caggtcttcc gcaaaggacg
     2101 ccacaaaatt ccgtcgctgc tggattataa gcttcccacc acgctcgata cgcccgatat
     2161 cgagaccatt ctgatcgaag tgcccgacca ggaaggtccc tacggcggca aagaggccgg
     2221 gcaagggccg ctcaatccga ccattcctgc catcgccaac gcggtctttg acgcgattgg
     2281 cgtgagtatc gatgaggtgc cgatcacacc agagaaagtc ctcaaagctc tgaaggctga
     2341 agcgaccacg acgcgcgcgg cagcccgacc acaggtccca cctgtaaagg cgccttcacc
     2401 ctggccaacc cgtctgatca agtggcagcc cggcgcgcaa gatggggcgt cacaagcagg
     2461 cgagcaagga aacggtcaca agccggtcca gaacgcaggc acgtttgcga cggccacggg
     2521 agagatttcc tgatgctgcg acttccacca tttcgttacc tggctccccg gacgatagaa
     2581 cagaccgtcc agatgcttgc tcaggaggga gagcaagcta tgctggtcgc gggcggcacc
     2641 gatctgtatc ccaatatgaa acgccgtcaa tttacaccgc cggttttgat cggtctgcgc
     2701 gccatcgcag ccctgaagct catcgatggc tcgccagagc agggaatgcg catcggcgcc
     2761 ggggtggcgc tctccactct ggcaagcaac ccacttttcc agcg
//
LOCUS       DASZAK010000215         6555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_431866, whole genome shotgun sequence.
ACCESSION   DASZAK010000215 DASZAK010000000
VERSION     DASZAK010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431866"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(191..823)
                     /gene="mutL"
                     /locus_tag="VGT44_03655"
     CDS             complement(191..823)
                     /gene="mutL"
                     /locus_tag="VGT44_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV2579926.1"
                     /translation="MTITQRMNIPRTPIQPLSQDVAERIAAGEVIERPVSVVKELVEN
                     ALDAGAREIRVEIRGGGLSLVRVSDDGIGVPASELEQALARHTTSKIRNVEDLHSLYT
                     LGFRGEALASIAAVSEVMLVSRALESEERGEESPAVSLTMRGGEVTQRGHAARLHPAP
                     VDEDRHMLAAALRQRAITHITAQTEILFFAHRFHDKRVQHLRQGQMVQFG"
     gene            complement(958..3681)
                     /gene="mutS"
                     /locus_tag="VGT44_03660"
     CDS             complement(958..3681)
                     /gene="mutS"
                     /locus_tag="VGT44_03660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV2579927.1"
                     /translation="MPSTSTPARRQYLQMKSQYPDAILLYQVGDFYETFDEDAVVAAR
                     VLQIVLTKRAYGEERVPLAGIPLHALDNYVGKLVQQGYKVAICDQVGEVGHGLVERAV
                     TRILTAGTLSEPNLLPARRNNFLIAASTSNIQTAMAAVDVSTGEFSVTWFAPDEMPGA
                     LEAELQRLAPTECLLVEGSRAGAFALPQETMTITQCPPYFFEVDAARARLCRLFGVQS
                     LDAYGCAHAPQAIAAAGAIVVYLEKMNAKLLALLTGLKTYHAAGYMTLDAHTQRNLEL
                     LQSARGGSTQGSLLGVLDRTITPMGARQMRRALCQPLLDLAELEARLDSVEELYESPA
                     LRSRFTMHLQRLGDIERIAGRARQGTAVLRETLAMREYLLAVPTLQNLLRGCNATMLV
                     ELANELDACPQVTTLIEQALARDDGEDEQGDSGRLIRAGFHAELDELIASIRESRRWV
                     ASLEARERERTGIKSLRVSYNKVFGYSIEVSNSKRDLVPPDYLRKQTLVNAERYITPE
                     LKEHEARIASAEARIEELERSIYADVLRQVAVYYPQMIATARALAQVDVLLSFAEVAA
                     HAGYTRPGLEQSGVIEITGGRHPVVERSLDGEAFIPNDTRMDEEARIVLLTGPNMAGK
                     STYLRQVALITLMAQMGSFVPARGARIGLVDRIFTRVGAEDDIASGKSTFMVEMEETA
                     AILRHATRHSLVILDEIGRGTSTYDGLAIARAVVEHLHNTTLARTLFATHYHELSAMA
                     DELSSLRVQMMAISEDEQDGVIFLHRVVPGSAGRSYGVHVAKMAGMPGGIVQRASEVL
                     NQLETAKTMAVALNGNGALDGHNNDNGYTRLRVADGNGTYSTGARIGAMRTTYTWQSE
                     EARMAASVLERDDGEHVELDTIDVSAITPLDALNLLYLLQKKRKRDYPTHL"
     gene            3846..4283
                     /locus_tag="VGT44_03665"
     CDS             3846..4283
                     /locus_tag="VGT44_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEV2579928.1"
                     /translation="MAAPRNAMEIRPGYRGRAFTSDLTGQEFWLVVDKGFQPMGLVIG
                     NCIYSMGAMRNWLAGIKSNFQGEIKEYSEIMYQARELALSRMQFEADQLSADGVIGVD
                     IKVEFMHNNEWMEVTAIGTAVRWVGSGANLPPTGQGRVTIPVG"
     gene            4297..5481
                     /locus_tag="VGT44_03670"
     CDS             4297..5481
                     /locus_tag="VGT44_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV2579929.1"
                     /translation="MAFDLARARNDTPGCEHVLHFNNAGASLMPQPVLAATIAHLQRE
                     AQMGGYEAADAASDAIEHSYDAVAALIGCSRDEVAIVENATRAWDMAFYSIPFKPGDR
                     ILTAISEYASNYIAYLQIARKTGAVVEAIPNDAYGQVSVEALRHILDQPDHRVRLIAI
                     THIPTNGGLVNPAAAIGAMAREHNILYLLDACQSIGQLPINVDQLGCDMLSATGRKYL
                     RGPRGTGFLYVRRAILDQLEPPLLDLHAATWTAPDRYELRPDARRFENWETNYAGKIG
                     LGVAIDYALAWGLDTTWRRVQSLAYQLRSQLAALPGVIVHDRGVTQCGIVTFTLDGHD
                     PEEIRLALAQQHINVSISERSSTLLDMDARGLESMVRASVHYYNSEEEIVRFCAAITS
                     LL"
     gene            5591..>6555
                     /locus_tag="VGT44_03675"
     CDS             5591..>6555
                     /locus_tag="VGT44_03675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2579930.1"
                     /translation="MNDRVGQLLGNYRLNSLIGQGGSGEVYLAEHIHLNTLVSIKILS
                     MQLASDETEGFKKEAQIIAHLVHPNIIRVLDYDIDNNLPYLVMDYAPNGNLRSRHPFG
                     TRLPADIIVSYVKQMASALQFAHEQDVVHRDVKPENMLIGRNDEILLSDFGIALVGSS
                     MNSRPPQNFTGTFYITGTPLYMAPEQITGNPGHASDQYGLGVIVYEWLCGSPPFHGSF
                     MALAMQHMVTPPTPLREKVPSISPDIEQVVLRALAKDAQDRFATIQEFADALEQAVLQ
                     SEVSEDTLAFKRSASEPVKTVDENDLNRTDPGYSNVPPAFLEDDT"
BASE COUNT         1317 a         2097 c         1774 g         1367 t
ORIGIN      
        1 aacggtgcgt gacctgttct ataacgtccc tgcgcgcctc aaattcatgc gttcggagcg
       61 caccgaggcc gcgcacatca tcctgctggc gcgacgctac gctgtaggct atccagaggt
      121 gcgttttacg ttggcgcaag atgaatatat cctggttcag acgagcgggt cgggcgatct
      181 cgcgacgacg ctagccgaac tgtaccatct gcccctggcg gagatgctgc acccgcttat
      241 catggaagcg gtgggcaaag aacaggattt ccgtttgagc ggttatgtgg gtaatcgcgc
      301 gctggcgcag agcagccgcc agcatgtgtc ggtcttcgtc aacgggcgct gggtgcagtc
      361 gcgccgcgtg tccgcgctgc gtcacctcgc cgccgcgcat cgtcagggag acggcgggcg
      421 attcctcgcc tcgctcctcg ctttccagcg cgcggctgac cagcatgacc tccgagacgg
      481 cggcgatact cgccagcgcc tcgccgcgaa agcccagcgt atacaaggaa tgcaagtctt
      541 ccacgttgcg aatcttgctc gtcgtatggc gcgccagggc ctgctccagc tcactcgcgg
      601 gaacgccgat gccgtcatca ctcacgcgca ccaggctcag accgccgccg cgtatctcca
      661 cccgaatctc gcgcgctccc gcgtccagcg cgttctcgac cagttctttg acgaccgaga
      721 caggccgctc gatcacttcg cccgccgcga tgcgctcggc gacatcctgc gacaggggtt
      781 gtatcggggt tcgcggtata ttcatgcgtt gtgtgatagt catgaggtca atgtaacttt
      841 ctcgatctgc atccgtaggt gccggtttac cgcgcacacc gccgattcat cggccaactg
      901 gcgcgaacgg taagcgacaa ggccgatgaa tcggcggtgt gcgcggtaaa ccggcaccta
      961 cagatgggtt gggtagtccc gcttcctttt cttctgcaac aggtacaaca ggttcagcgc
     1021 atccaacggc gtgatcgcgc tcacatcaat cgtgtccagt tcaacatgct ccccatcgtc
     1081 ccgttccagc acgctagccg ccatacgcgc ctcttcgctc tgccaggtat acgttgtgcg
     1141 catagccccg atgcgcgcac ctgtgctgta agtgccgttc ccgtcggcca cgcgcagtcg
     1201 cgtatagcca ttatcattgt tatgtccatc gagcgcaccg ttgccgttca gcgcgacagc
     1261 cattgttttc gccgtctcaa gctgattcag cacctcgctc gcgcgctgca cgatgccgcc
     1321 gggcattcca gccattttcg ctacatgcac accatagctg cgcccggcgc tgcctggcac
     1381 gacacgatgc aggaagatga caccgtcctg ctcatcctcg cttatcgcca tcatctgtac
     1441 gcgcagcgag gacaactcat cggccatcgc cgacagctcg tgatagtgcg tggcgaagag
     1501 tgtgcgcgcc agggtagtgt tatgcaggtg ttcgacgacg gcgcgcgcga tagccaggcc
     1561 gtcgtaggtg ctggttcccc ggcctatctc gtccaggatc accaggctgt ggcgtgtggc
     1621 atggcgcagt atcgcggcgg tctcctccat ctccaccata aaggtgcttt tgcccgacgc
     1681 gatatcgtct tccgctccga cgcgcgtgaa gatgcgatca accagcccga tgcgcgcgcc
     1741 gcgagcaggc acaaagctgc ccatctgcgc catcagcgtg attagcgcta cctgccgcag
     1801 ataggtcgat ttgcccgcca tatttggtcc tgtcagcagc acaatgcgcg cttcctcgtc
     1861 catgcgcgta tcgttaggga taaacgcctc gccgtccagc gagcgttcaa ccaccggatg
     1921 ccgcccgcct gtaatctcga tgacgccgct ttgctccagc cctgggcgtg tgtagccagc
     1981 gtgcgccgcg acctcggcaa agctcagcag cacatccacc tgcgccagcg cgcgggcggt
     2041 ggcgatcatc tgtgggtaat acaccgcgac ctggcgcagc acatcggcgt agatgctgcg
     2101 ttccagttcc tcgatgcgcg cctccgcgct ggcgatgcgc gcctcgtgtt ccttcaattc
     2161 aggcgtgata tagcgttccg cgttgaccag cgtctgcttg cgcaggtagt caggcggcac
     2221 caggtcgcgt ttgctgttgc tgacctctat ggagtagccg aagactttgt tgtagctgac
     2281 acgcagcgat ttgatgccgg tgcgctcgcg ttcgcgcgcc tccaacgagg ccacccagcg
     2341 ccgcgactcg cgaatggagg cgatcagttc atccagttcg gcgtggaagc ccgcgcggat
     2401 cagtcgtccg ctgtctccct gttcatcttc tccgtcgtcc cgcgctaatg cctgttcgat
     2461 cagcgttgtg acttgtgggc aggcatccag ctcgttcgcc agctcaacca gcatcgtcgc
     2521 attgcagccg cgtaacaggt tctgcagcgt tggcaccgcc agcaggtact cgcgcatggc
     2581 cagcgtttcg cgcagaaccg ccgttccctg ccgcgcacgc ccggcgatac gctcgatatc
     2641 gcccaatcgc tgcaggtgca tcgtgaagcg gctgcgcagc gcaggacttt cgtagagttc
     2701 ctcgacgctg tccagccgcg cctccaactc cgccaggtcg agcaggggct ggcaaagcgc
     2761 tcgccgcatc tgccgcgcgc ccatcggcgt gattgtgcgg tccagcacac ccagcaggct
     2821 gccctgtgtc gagccgccgc gcgcgctctg taacagttcc aggttgcgct gtgtatgcgc
     2881 atccagcgtc atataaccag cggcgtgata cgtcttcagg ccggtcagca gcgccagtag
     2941 cttcgcgttc atcttttcca ggtacactac gatagccccg gctgccgcga tggcctgtgg
     3001 cgcgtgcgcg catccatacg catcgagtga ttgcacaccg aacagccgac acagccgcgc
     3061 gcgcgccgcg tccacctcga agaaataagg cggacactgt gtgatcgtca tcgtctcctg
     3121 cggcagagca aacgccccgg cacgactccc ctcgaccagc aggcattctg taggcgcgag
     3181 tcgctgcaac tccgcctcca gcgcccccgg catttcatcc ggcgcgaacc atgtcacact
     3241 gaactcacct gtgctgacat ccaccgctgc catcgccgtc tgaatattgc tagtcgatgc
     3301 ggctataaga aagttattgc ggcgcgctgg taaaagattc ggctcagaga gcgtgccagc
     3361 cgtcaggatg cgcgtcactg cccgttctac caatccgtgt cccacctcgc ccacctggtc
     3421 gcagattgct actttgtagc cctgctggac aagcttgccc acgtagttat ccagcgcatg
     3481 tagggggatg cccgccagtg gcacgcgctc ctccccatag gcgcgtttcg tcagtacgat
     3541 ttgcagtacg cgcgccgcca caaccgcgtc ctcatcgaac gtttcgtaaa aatctccaac
     3601 ctggtacagc aaaatagcgt ccgggtactg gctcttcatt tgtagatact gcctgcgcgc
     3661 gggcgtggat gtcgatggca tagactacat tctcgacttc accatagata cacctgttgc
     3721 ttttattgaa acatctgtac tttaaactat tctgtgccac ttgtcaaaaa atggtatttg
     3781 ggctatgctt acagccatca ggctctattc catttaacca catgaaagcg aggcatttga
     3841 cacagatggc agcaccacgt aacgcaatgg aaatccggcc cggctatcgc gggcgcgcct
     3901 ttacctccga tctgaccggc caggagttct ggctggtagt agacaaaggt ttccagccga
     3961 tgggactggt gattggcaac tgcatctatt cgatgggcgc catgcgcaac tggctggccg
     4021 gcatcaaaag caattttcaa ggcgaaatca aggaatactc cgaaatcatg taccaggcgc
     4081 gcgaactggc gctgagccgc atgcaattcg aggccgacca gctcagcgcg gacggcgtga
     4141 tcggcgtcga tatcaaagtc gagttcatgc acaacaacga atggatggaa gtaaccgcca
     4201 tcggcaccgc cgtgcgctgg gtaggcagcg gcgctaattt gccccccacc ggccagggcc
     4261 gcgtcaccat cccggtaggg taatgaaagg ctagccatgg cctttgacct tgcgcgagcg
     4321 cgcaacgaca cacccggctg cgagcatgtg ctgcatttca acaatgctgg cgcgtccctc
     4381 atgccgcagc ccgtcctcgc ggccaccatc gcccacctgc aacgtgaggc gcagatgggc
     4441 ggctacgagg cagccgatgc tgcaagcgac gccatcgagc atagctatga tgcagtcgcg
     4501 gcactgatcg gctgctcgcg cgacgaggtc gccatcgtcg aaaacgccac ccgcgcctgg
     4561 gatatggcct tttattctat ccccttcaag ccgggggacc gcatcctgac cgccatctcg
     4621 gaatacgcca gcaactacat cgcctacctg caaatcgccc gcaaaaccgg cgcggtggtc
     4681 gaggccatcc ccaacgacgc ttacgggcaa gtctcggtcg aggccctgcg ccacatactc
     4741 gaccagcccg accaccgcgt gcggctcatc gccatcacgc acattcccac caatggcggc
     4801 ctcgtcaacc cggctgccgc catcggcgca atggcgcgcg agcataacat cctctacctg
     4861 ctcgatgcct gccaatccat cggccagttg cccatcaacg tagaccagct cggctgcgac
     4921 atgctctccg ccaccgggcg caaatacctg cgcggcccgc gcggcaccgg cttcctctac
     4981 gtgcgccgcg ccatcctcga ccagctcgaa ccgcccctgc tcgacctgca cgccgccacc
     5041 tggaccgcgc ccgaccgcta cgaattgcgc cccgacgcgc gccgcttcga gaattgggaa
     5101 accaactacg ccggaaaaat cggcctgggc gtcgccatcg actacgctct cgcctgggga
     5161 ctcgacacca cctggcggcg cgtccaatcc ctcgcctacc agctgcgctc gcagctcgct
     5221 gctctccccg gcgtgatcgt gcatgatcgc ggcgtcaccc agtgcggcat cgtgactttc
     5281 actctcgatg gacacgaccc ggaagaaatc cgcctcgctc tcgcccaaca gcatatcaac
     5341 gtatcgatct cggagcgcag cagcaccctg ctcgatatgg acgcgcgcgg cctggaaagc
     5401 atggtcaggg catcggtgca ttattacaac agtgaggaag aaatcgtgcg gttttgcgcg
     5461 gcaataacct cgctactgta aatacctgat gaagctaatt ttgcttgaca tgttcgcatt
     5521 atacttctac aatacatcca aacgacccaa gatgtgctga gaattgacca ctcaaaaggg
     5581 agtgtcaatt atgaatgatc gcgtgggaca actcctggga aattatcgcc tcaacagttt
     5641 gataggtcag ggaggatcgg gtgaagttta cctggccgaa catattcacc tgaatacact
     5701 tgtgtctatc aaaatattga gtatgcaatt ggcaagcgat gaaaccgaag gattcaaaaa
     5761 ggaagcgcaa atcattgctc acctggtgca tccaaatatt attcgagtac ttgattatga
     5821 tatagacaac aacctgccct acctggtaat ggactatgcc cctaatggaa acctgcgaag
     5881 ccgccatccc tttggtaccc gtcttcctgc tgacattatc gtatcctatg ttaagcaaat
     5941 ggccagcgct ttacaattcg cccacgaaca agatgttgtt caccgtgatg tcaaacctga
     6001 aaatatgctg atagggcgaa atgatgagat tttactcagc gattttggta tagcccttgt
     6061 gggttccagt atgaactctc ggccaccaca aaattttaca ggcactttct atatcacggg
     6121 cactccttta tatatggctc ccgagcagat caccggcaat cccggtcatg caagtgatca
     6181 gtacggactg ggtgtgattg tctacgaatg gttatgtggc tcgcctcctt ttcacggctc
     6241 tttcatggcg cttgccatgc agcatatggt tacaccgcca actccacttc gggaaaaagt
     6301 tccgtcgatt tcacccgaca tcgagcaagt agtcctcaga gcgctcgcta aagatgctca
     6361 agatcgcttt gccactattc aggagtttgc cgatgcgctc gaacaagctg tactacaatc
     6421 ggaggttagc gaagatacgc ttgcgttcaa acgatcagcg tctgaacctg taaaaacagt
     6481 tgacgagaac gatctgaaca ggactgatcc tgggtactct aacgttcctc cggcattcct
     6541 ggaagatgac acttt
//
LOCUS       DASZAK010000216         2048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_5549, whole genome shotgun sequence.
ACCESSION   DASZAK010000216 DASZAK010000000
VERSION     DASZAK010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5549"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2048)
                     /locus_tag="VGT44_03680"
     CDS             complement(<1..>2048)
                     /locus_tag="VGT44_03680"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2579931.1"
                     /translation="VQAANTTQVLPAEQMVAPTVNGTQAGTAPADATVPVAPVAPPPL
                     QQAQPEPSMATFVPTAPAVQETAMPAPIAPPPPDLAQQQAALPTQVPAPAPEAQPVLS
                     PVIQTPLTPSAEEMLSQMAGLSSKDGVLKNVADSLLVSTANLPEQVRMQVLQSMQDIQ
                     RYIDHGLLTPATEECLKIVSIAPQYLDIHQVLCEIYVRQGKVEQAVTKYGILIDTYVV
                     NGRVDDAIATYRRILQLEPNNLTYRVRLINLLAAHGSKEDLLRERSLAAESYLRLGYM
                     DRALTELEQALQESPTSVPTRLNYALAMQKLGRTQQAVAEYQRVLQVDPRNLTALVRW
                     HIAMITGVNASRATTLEILSRIRWQLRGEGQKQYEAVMREYNAAAETYPNNFDVRFAQ
                     GQLHQQAGNYDRAIDAFQFATRDSSYEIMARASAAQCLLTQGKPEAAIQQLEQALHSA
                     RTNARGSLDPTVWAARPREDGEEHQAPEVEISLMLAKAYGRVGKQEQMQEILRQLKQS
                     TPQRDEVTSTLAEISVRQGDLNGALQEYTDLAKQYRSKRQPDNALSVLREMIRIAPQD
                     PRAHAELADLHINRGHLDDGLAELRLLADIYLRNDDLTLAGETLGRVANIYGELDNSD
                     DAFMYYRRAAELLPGNMDLLREVVSFCWQAGRNKEAIHYQEIIARHYFETRQVKESVA
                     "
BASE COUNT          338 a          659 c          632 g          419 t
ORIGIN      
        1 cggccaccga ctctttgacc tggcgcgtct cgaagtagtg gcgggcgatg atctcctgat
       61 agtgaatggc ctccttgttg cgcccagcct gccagcagaa gctcaccact tcacgcagca
      121 agtccatatt gccgggcagc agttcggcgg cacggcgata atacatgaag gcgtcgtccg
      181 agttgtccag ttcgccataa atgttggcga cgcgccccag cgtttcgccc gccagcgtca
      241 ggtcatcgtt gcgcaggtag atgtccgcca gcagccgcaa ttccgcgagg ccatcgtcca
      301 ggtggccgcg attgatgtgc agatcggcca gttcggcatg agcgcgcggg tcctgtggag
      361 cgatgcggat catctcgcgc agcacgctca aggcgttgtc gggttggcgt ttgctgcggt
      421 actgcttcgc caggtcggtg tactcttgca gcgcgccgtt gagatccccc tggcgcacag
      481 agatttccgc cagcgtcgat gtgacctcgt cccgttgggg cgtcgattgt ttcagctggc
      541 gcaggatctc ctgcatttgc tcctgcttgc cgacccgccc atacgccttt gccagcatga
      601 gcgaaatctc cacctccggc gcctggtgct cttcgccatc ttcgcgcggg cgagccgccc
      661 acaccgtcgg gtcaagcgag ccacgcgcgt tcgtgcgcgc gctatgcagc gcctgttcca
      721 gttgctggat ggctgcctcc ggcttgccct gcgtcagcag gcactgcgcg gcgctggcgc
      781 gcgccatgat ctcataggaa ctatcgcgtg tggcaaactg gaacgcatcg atggcgcgat
      841 cataattgcc cgcctgctgg tgcagctgcc cctgggcaaa gcgcacatca aagttgttgg
      901 ggtaggtctc cgctgccgcg ttgtattcgc gcatgaccgc ctcatattgc ttctgcccct
      961 cgccgcgcaa ttgccagcga atgcggctca ggatttcgag cgtggttgcg cgactcgcgt
     1021 tcaccccggt aatcatcgca atatgccagc gcaccagcgc ggtcaggttg cgcgggtcga
     1081 cctgcaaaac gcgctggtac tcggccaccg cctgctgggt gcgccccagc ttctgcatag
     1141 ccagggcata gttcaggcgc gtaggaacgc tcgtcggtga ttcctgcaat gcctgttcca
     1201 gctcggtcag agcgcgatcc atgtagccca ggcgcaagta cgactctgcc gccaggctgc
     1261 gctcgcgcag caaatcctct ttactgccgt gcgccgccag caggttgatg agacgcacgc
     1321 gatacgtcag gttattcggt tccagttgca ggatgcggcg gtaggtggcg atagcgtcgt
     1381 cgacgcggcc attcaccacg taggtgtcga tcagaatgcc atacttggtc accgcctgct
     1441 ccaccttgcc ctgacgcaca taaatttcgc acagcacctg gtgaatatcc aggtactgcg
     1501 gcgcgatact gacgatcttc aggcattctt ccgtcgctgg cgtcagcaac ccatgatcga
     1561 tgtagcgctg gatgtcctgc atcgactgca agacctgcat ccgcacctgt tccggcagat
     1621 tcgccgtgct gaccagcagg gaatccgcca catttttgag gaccccgtcc ttgctcgaaa
     1681 gccccgccat ttgcgagagc atctcctccg cgctgggcgt caggggggtc tgtatcaccg
     1741 gcgagagaac cggctgggct tccggcgcgg gagccggtac ttgagtaggc agcgcagctt
     1801 gttgctgcgc caggtcaggc ggcggcggcg cgataggcgc gggcatcgcg gtctcctgga
     1861 ccgcaggcgc agttggaaca aacgtcgcca tcgatggctc aggttgcgcc tgctgcaacg
     1921 gcggtggcgc gaccggggca actggaacag tagcatccgc cggagccgta cccgcttgcg
     1981 taccgttaac cgtcggcgca accatctgct ccgctggcag cacctgcgtg gtattggcag
     2041 cctgcacc
//
LOCUS       DASZAK010000217         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_40681, whole genome shotgun sequence.
ACCESSION   DASZAK010000217 DASZAK010000000
VERSION     DASZAK010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40681"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="VGT44_03685"
     CDS             <1..515
                     /locus_tag="VGT44_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579932.1"
                     /translation="YAVHCSGISAPNCSGSCALGTREGPSTIYTEPATGGALQSMFTS
                     QALGIAQVRSITSSLLLLNVENFSQNHPVDTSQNGLWVVHSDGSGLTRLTSVTANTFT
                     ALCQFSQTPWSNVSRDSSMYAYQTSLDNSTAGTTAYTLSYGSLNGGATQSFASFTDNT
                     QLAIIGWTTM"
     gene            599..1372
                     /locus_tag="VGT44_03690"
     CDS             599..1372
                     /locus_tag="VGT44_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2579933.1"
                     /translation="MPFEYITLEYAGDYATITMNNPKRRNALSLRHMQELTAAFREVG
                     DSATRGVILAANGPVFSAGHDFADMAGQDLIAMRKLLKVCTVMMDTIQSIPQPVLARV
                     QGLATAAGCQLVATCDLAVASSEAGFATPGGKGGWFCTTPMVAVSRNIGRKRALEMLL
                     TGEAIDAQTAADWGLVNRVVAPERLVEEAQRLLEAATHGSFFSKGIGKQAYYAQIEMP
                     QPLAYAYAQEVMASASQIPDAQEGMQAFLEKRKPNWQNP"
     gene            1375..2163
                     /gene="fabG"
                     /locus_tag="VGT44_03695"
     CDS             1375..2163
                     /gene="fabG"
                     /locus_tag="VGT44_03695"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEV2579934.1"
                     /translation="MGKSILDNKIAVITGGGKGIGRVIALACASAGANLVLASRSEDA
                     LRDTRAEVERMGREALVVPTDITQEASVRNLAERALAHFGRVDILVNNSGITGPVKPL
                     WEVTFDEWQETFDINVTGAFLCCRAFLPAMIERRAGSIIFISSMTGKRPLYGRATYGA
                     GKMALVGMARTLAWETGPYGIRVNVVSPGAVAGERLEGVFRSQAEVEHISVDEARRNF
                     TETAPLNRLVSPSDIADAVVFLASDRAASITGEDLNVSAGIVMY"
BASE COUNT          453 a          735 c          624 g          379 t
ORIGIN      
        1 tctacgctgt gcattgcagc ggcatcagcg cgcctaattg cagcggttca tgtgcgttgg
       61 gaacccgcga aggaccaagc acgatttata ccgaaccggc aaccggcggc gcgctgcaaa
      121 gcatgttcac cagccaggcg ctcggtatcg cgcaggtacg ctccatcacc agttcattac
      181 ttctattgaa tgtggagaat tttagccaga atcacccggt cgataccagc cagaacggcc
      241 tctgggttgt gcattccgac ggatcagggc tgacacgcct caccagcgta accgccaata
      301 ccttcaccgc cctgtgccag ttctcgcaaa ctccctggtc gaacgtctcg cgcgacagca
      361 gcatgtacgc ctatcagacg agcctcgaca actctaccgc cggaaccacc gcgtacacgc
      421 tctcgtatgg ctcgctcaac ggcggcgcaa cacaatcgtt cgcctcgttc accgataaca
      481 cacaactggc catcatcggc tggacaacga tgtagcgtac aaccacaaga aaaatacccc
      541 acacatggta tgatattcac agatcatccg tgcaagtaca cccgaaagga gaaccgacat
      601 gccgtttgaa tacatcacat tggaatacgc cggcgattac gccaccatta ccatgaacaa
      661 tcctaaacgc cgtaacgccc tgtcgctacg gcatatgcag gaactgacgg cggcattccg
      721 cgaggtcggc gacagcgcca cgcgcggggt gatcctggcg gcgaacgggc cggtcttttc
      781 ggcaggccac gacttcgcgg acatggcggg acaagacctg atcgccatgc gcaagctctt
      841 gaaggtctgc accgtcatga tggacaccat ccaatcgatc ccgcagcccg ttctcgcgcg
      901 cgtccagggc ctggccaccg cggcaggatg ccagctcgtc gccacctgcg acctggccgt
      961 tgcttccagc gaggccggtt tcgccacgcc cggcggcaag ggcggctggt tctgcacaac
     1021 gccaatggtg gctgtcagcc gcaatatcgg tcgcaagcgc gcgctggaaa tgctgctgac
     1081 cggcgaggcc atcgatgccc agacggcagc cgattggggc ctggtcaacc gcgtcgtcgc
     1141 gcccgaacgg ctcgttgagg aagcgcagcg cctgctcgaa gcggccacac acggcagttt
     1201 cttctccaaa ggcatcggca agcaagccta ttacgcccag atcgaaatgc cgcagcccct
     1261 ggcctatgcc tacgcccagg aggtcatggc ctccgcgtcg caaatccccg acgcccagga
     1321 aggtatgcaa gccttcctag agaagcgcaa accaaactgg cagaatccat aaacgtggga
     1381 aaatctatcc tcgacaacaa aatcgctgtg attaccggcg gtggcaaggg catcgggcgc
     1441 gtcatcgcgc ttgcctgcgc ctctgcgggt gccaacctcg tacttgcctc gcgctcggag
     1501 gatgccctgc gcgacacgcg agccgaggta gaacgcatgg gtcgcgaggc gctggtcgtg
     1561 ccgaccgaca tcacgcagga agccagtgtg cgcaacctgg cagaacgcgc cctggcgcat
     1621 ttcgggcgcg tcgatatcct cgtcaacaat agcggcatca ccggcccggt caaaccgctc
     1681 tgggaggtca cgttcgacga gtggcaggag accttcgaca tcaacgtcac cggcgcgttc
     1741 ctgtgctgcc gcgccttcct gcccgccatg atcgagcggc gggcaggctc gatcatcttc
     1801 atctcctcta tgacgggcaa gcgaccgctc tatggccgcg caacctacgg cgcgggcaaa
     1861 atggcgctcg tcggcatggc acgcacgctg gcctgggaaa cgggccccta cggcatccgc
     1921 gtcaacgtcg tctcccccgg cgcggtcgca ggcgagcggc tcgaaggcgt cttccgctcg
     1981 caggccgagg tggaacacat cagcgtggac gaggcgcgcc gcaacttcac cgaaaccgcc
     2041 ccgctcaatc gcctggtttc ccctagcgat atcgccgacg ccgtcgtctt cctggcctcc
     2101 gaccgggccg cctcgatcac cggagaagac ctgaacgtct cggctggcat cgtgatgtat
     2161 tgaaaggtgg gctttagcct ccttctcagt c
//
LOCUS       DASZAK010000218         2681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_360889, whole genome shotgun sequence.
ACCESSION   DASZAK010000218 DASZAK010000000
VERSION     DASZAK010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360889"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(316..1248)
                     /locus_tag="VGT44_03700"
     CDS             complement(316..1248)
                     /locus_tag="VGT44_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2579935.1"
                     /translation="MASIALLTLLLLEVVDYIQKQLLSLPAQAIRGVGMKIGDFSKLS
                     QVSIKALRYYDEMGLLKPIETDRFTGYRNYSVGQLPRLNRILALKDMGFSLEQIAQVL
                     NQGVSSEQLRGMLRLKRAELQQHIADEQARLARVEARLRDIAMEDTMTDNQVANYDVV
                     IKQVEPQLVASVRDTLPAYTAVGRLFDEVYGYLSRSGVAGLAAIGVAIWHDNEYKTSD
                     IDGEAAVLLKQPVAEGERIKVYELPGATMASVIHKGAFNALSQAYEAAWRWIEANGYK
                     VIGPNREVYLYCTQPVRQDDESYVTEIQFPVEKM"
     gene            1347..1832
                     /locus_tag="VGT44_03705"
     CDS             1347..1832
                     /locus_tag="VGT44_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579936.1"
                     /translation="MSFQICIKTEKSLQQLATEIRNLFSLPPFKMSTFSGDAYCQFEV
                     LGLVILIHQADEEDRDVEVMHYPYCFDVQMSFVDHDLDTDTLEYRLQPYYAQLLSFHL
                     GLETAYHEKHKVDRAWHIRYHFCSKNPKWNANILYGEPGWEPAIIEESPSPWRAMHPV
                     F"
     gene            1856..2296
                     /locus_tag="VGT44_03710"
     CDS             1856..2296
                     /locus_tag="VGT44_03710"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEV2579937.1"
                     /translation="MSILDIAVGQELIPFVKPPIAQEQLKRYAEASGDFNPIHLDEEA
                     AHRVGLDSIIAHGMLSMAFLGQFLQQQLTGIPGARIADLRVRYVSMVRLGDMLTCLGV
                     LKERATQGDEQVLTVECWAQNQHGEKVTTGEAVLIVPLGAEVKA"
     gene            2293..>2681
                     /locus_tag="VGT44_03715"
     CDS             2293..>2681
                     /locus_tag="VGT44_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2579938.1"
                     /translation="MTERRARPMGLADLRLIYDLSIFNCQHFDAYPALTYEDLQQDQV
                     RVFTNIEIAREATQLAVGLRSLCIQKGDRVIVMMLNCPEVIISYQAIARAGGIIIPVM
                     PLLKPPEVRYIAENSAAKGVITSPILLP"
BASE COUNT          609 a          787 c          680 g          605 t
ORIGIN      
        1 agctgcccaa agtcgaccag ttccccagcg cctgatccca acaatcacca gaatcagccc
       61 cacaatcacg cacggaatga agatgaagta aacatcaaag ccgagtagcc ctatgccgac
      121 aagagctcca gcgatgagcc agtagagtac ctgccacggc cgtctatttg ccatgtgctt
      181 tacctccctg ccagaggatg agtcgtgttt ccctaagtat aacggcaggg ctttttattt
      241 gacagtgggt ctacacaccg gaaacggtga ctgtagaccc acctttttga accgacgaac
      301 gtcacatcaa cattgctaca tcttctcgac cgggaactga atctccgtca catacgattc
      361 gtcgtcctgg cgcacgggct gggtgcagta caggtagact tcgcgattgg ggccgatgac
      421 cttgtagcca ttggcctcga tccagcgcca ggcggcctcg tatgcctggc tgagggcgtt
      481 gaaagcgccc ttatgaatga cgctggccat cgtcgcgcca ggaagctcgt agactttgat
      541 acgctcgcct tcagcaacgg gctgcttcag caagacagca gcttcgccat cgatatcgct
      601 ggtcttatac tcgttgtcgt gccagatcgc caccccaatc gccgcgagtc cagcgacgcc
      661 agagcgcgat aggtacccat acacttcatc gaagaggcga ccgaccgcag tataggcggg
      721 cagcgtatcg cgcacgctgg cgaccaactg tggttcaacc tgtttgatca caacatcgta
      781 gtttgcaacc tgattgtctg tcatagtatc ctccattgca atatctctga gccgggcctc
      841 aaccctcgcc aatcgcgcct gttcgtcagc aatgtgttgc tgcaattcgg cgcgtttgag
      901 gcgcagcatc ccacgcagtt gctctgatga aacgccttga ttgagcacct gcgcgatctg
      961 ctccagcgag aaccccatgt ccttgagcgc caggatgcga ttgagcctgg gaagctgacc
     1021 gaccgagtag ttgcgatagc cggtgaagcg atcagtctcg atcggtttga gcaatcccat
     1081 ctcatcgtaa tagcgcaggg ccttgatcga aacctggctc agcttcgaga aatctccgat
     1141 tttcatacca actccgcgga tagcctgcgc tggcaggctt agcaattgct tctggatata
     1201 gtctacaacc tccagtagca ggagagtcaa gagggcgatg ctggccatct tgctatcgct
     1261 tgacaccatt ttctctgcgg cgtaagatga tgcaagtaaa ctgtacacta ccagggacta
     1321 actcaccttc tttgaaggag atctgtatgt cgttccaaat ctgtattaag actgagaaat
     1381 ccctacaaca actggcaaca gaaatacgca atctcttctc tctacctcct tttaagatga
     1441 gtaccttttc gggtgatgcc tattgccagt tcgaggtgtt gggattggtc attctcattc
     1501 accaggccga cgaagaggat cgcgatgtgg aggtgatgca ttacccctat tgcttcgatg
     1561 tacaaatgtc gttcgtcgat cacgacctgg ataccgatac gttggaatat cgtctccagc
     1621 cctattatgc ccagctactc agctttcatc tcggcctcga gaccgcctac catgaaaaac
     1681 ataaggtcga tcgcgcctgg catatccgct atcatttctg ttccaaaaat ccaaaatgga
     1741 atgcgaatat tttgtatggc gaacccggct gggaacccgc gatcatcgaa gagtctccca
     1801 gcccctggcg agccatgcat cccgtctttt gattgttcca caaatggagg caagcttgag
     1861 cattttagac atcgccgtcg gccaggagct gataccgttt gtcaaaccac ctatcgcgca
     1921 ggagcagttg aagcgctacg ccgaagcgtc gggtgacttc aatcctattc acctggacga
     1981 ggaggccgcg caccgcgtcg gcctggatag catcatcgcg catggcatgt tgagtatggc
     2041 cttcctcggt cagtttctcc agcagcagct caccggcatc cccggcgcgc gcatcgccga
     2101 cctgcgggtg cgctacgtca gtatggtgcg cctgggcgat atgctgacct gcctgggcgt
     2161 ccttaaagag cgcgcgacgc agggcgacga gcaagtctta acggtagagt gctgggcgca
     2221 gaaccagcat ggcgaaaaag tcactaccgg cgaagccgta ctgatcgttc ccctcggcgc
     2281 ggaggtgaag gcatgaccga acgccgcgcc cggccaatgg gactcgcaga cctgcgtttg
     2341 atctacgacc tctccatttt taactgccag cattttgatg cctatcccgc gctgacctac
     2401 gaggacctgc aacaggacca ggtgcgcgta ttcaccaata ttgagatcgc cagggaagca
     2461 acccaactgg ctgtcggcct gcgctcgctg tgcatccaga agggcgaccg cgtcatcgtc
     2521 atgatgctca actgccccga agtcatcatt tcataccagg cgattgcccg tgcaggcggc
     2581 atcattattc ccgtcatgcc tctcctcaaa cctccagaag tacgttatat tgcagaaaac
     2641 tccgccgcaa agggagtcat caccagtcct atacttctcc c
//
LOCUS       DASZAK010000219         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_254398, whole genome shotgun sequence.
ACCESSION   DASZAK010000219 DASZAK010000000
VERSION     DASZAK010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254398"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VGT44_03720"
     CDS             complement(<1..804)
                     /locus_tag="VGT44_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEV2579939.1"
                     /translation="MSTTIQEITDRDQWNGFLTCQPSTSPGHLLQSYEWGELNKYLGA
                     RVYRLGALEQGRMTGAMLLTVSPVPLSVPGLRWNWLYSARGPTVASPDSPALPALIDY
                     AQQYIARTEHAVVLRLEPNIADDTPGQDAWLAAYARLGFQTNPNAIHGRRSWALDIRP
                     DADTLLANFKMNWRQNVRVAERKGVIIREASSDADFDAYYDLLKLTSERDAFFIHQKD
                     YHREILRHFASKGDAVLFLAEHEGEAIAAKMLIRFGDWCWDMFGASSNNK"
     gene            complement(804..1373)
                     /locus_tag="VGT44_03725"
     CDS             complement(804..1373)
                     /locus_tag="VGT44_03725"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2579940.1"
                     /translation="MTEDTTHLQNPFLIGERLYLRPLEPAQDNHLYSTWMNDEEIRKY
                     FSIYPTSDARGKERLEQLYRDGKHIIFGVALKSDNTLIGLVGLKDINTINQSAEFYVI
                     IGDRSAWFKGYGTEATKLMFRYGFMELNLNRIQTQDMDENIGGWRADEKAGMKYEGTL
                     RQVILRFGTYHDVRVYCILRSEYLESLEK"
     gene            complement(1370..3952)
                     /gene="lon"
                     /locus_tag="VGT44_03730"
     CDS             complement(1370..3952)
                     /gene="lon"
                     /locus_tag="VGT44_03730"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV2579941.1"
                     /translation="MNDTEHPDTLMDQRSPYPLVALRDMVVFPRTRTTLSMMREKLIR
                     AVEEAMMQPEHLLITAAQQEAEIDDPQPKDIYSVGTLVEVTTMHRQQDGSFQILLQGL
                     RRVQIEEFVEQEPFLRVIVEPLIEEQPHGSMTDAVVRHATNLFERYAQLNRKYTAEDI
                     TSIVSQKTAARLSDTLATFVVTDFHQQQELLEMLDPLARLEKICVLMGNEIEILELES
                     TIRTRVRSQVDRSQKEFYLREHLRAIQEELGMEGASEANELRDRVNELAAQGMPLDVT
                     NKTRKEIDRLERTPPQSADIASIRSYIDWILALPWSERTDDHFDIETTRRILEEDHYG
                     LEGIKERILEFLAVRQLRQRRASRAGVVRQESQGQILCFIGPPGVGKTSLGQSIARAL
                     GRKFARIALGGVSDEAEIRGHRRTYVGALPGRIIQSMKTVGVRNPVFLLDEVDKLSSE
                     YRGDPAAALLEVLDSAQNNVFVDHYLEVPFDLSEVFFICTGNVRYQVPRPLLDRMDVI
                     DLPGYILEEKINIGSRHLLPRVLAEHGIAPEQLKIPQAVMQHIVTDYTREAGVRALER
                     QLTTICRKSARRIFEKPDTHIRVTTHNLENYLGAPRYLESPINHTVQVGVALGLAVTD
                     AGGMVLPVEVVTMPGKGEVLITGQLGEVMRESAFAALSYIRSRAKDLELDPNFQDSTD
                     MHIHMPENALPKEGPSAGIAIATALISALTHRPVRGDLAMTGEITLRGRVLAIGGLKE
                     KTLAAHAANIFQLLIPADNKKDLAEIPAKIRQHMTFITVETMDHVIESALLAAPADED
                     QEQNQNGRDEEAAPEPPPHHLDERNPGSRRRHTPDVQEGEDEAGGHRPFIIPPNDSNR
                     ELYP"
BASE COUNT          696 a         1118 c         1363 g          907 t
ORIGIN      
        1 tttgttgttg gatgacgcgc cgaacatgtc ccagcaccag tcgccgaaac ggatcagcat
       61 tttggcggcg atggcctcgc cctcgtgttc ggcgaggaag aggacggcgt cgcctttgct
      121 ggcgaagtga cgcaggattt cgcggtgata gtccttctgg tgaatgaaga aggcgtcgcg
      181 ctcgctggtg agcttgagca ggtcgtagta ggcgtcgaaa tcggcgtcgc tgctggcttc
      241 gcggatgatg acgcccttgc gttcggccac gcgcacgttt tgccgccagt tcatcttgaa
      301 gttggcgagg agtgtatcgg cgtcgggtcg gatgtcaagc gcccagctgc ggcggccatg
      361 gatggcgttg ggattggtct ggaagcccag ccgggcatag gcggcgagcc aggcatcctg
      421 gccgggcgta tcgtcggcga tatttggttc caggcgcagc acgacggcgt gttctgttct
      481 ggcgatatac tgctgcgcgt agtcgatcag ggcgggcagg gcgggcgagt cgggcgaggc
      541 gacggtgggg ccgcgcgcgc tgtagagcca gttccagcgc aggccgggaa cggagagcgg
      601 cacgggcgag acggtcagca gcatcgcgcc ggtcatgcgc ccctgttcga gcgcgcccag
      661 gcgatagacg cgcgcgccca ggtatttgtt cagctcgccc cattcgtagg actgcaagag
      721 atggccggga gacgtggaag gctgacaggt aaggaacccg ttccactggt cgcggtccgt
      781 gatttcctgg atggtggttg acattacttt tcaagactct caagatattc gctgcgcagg
      841 atgcagtaga cgcgcacgtc gtgatatgtg ccgaagcgca ggatgacctg gcgcagggtg
      901 ccttcgtact tcataccggc cttctcgtcg gcgcgccagc cgccaatgtt ctcgtccatg
      961 tcctgcgtct ggatgcggtt gaggttgagt tccatgaagc cgtagcggaa catcagcttc
     1021 gtcgcctcgg tgccgtagcc tttgaaccag gcgctgcggt cgccgatgat gacgtaaaat
     1081 tccgcgctct ggttgatggt gttgatgtcc ttcaacccga ccagcccgat cagggtgtta
     1141 tcacttttga gcgccacgcc gaaaatgata tgcttgccat ctctatataa ctgctccagg
     1201 cgctcttttc cgcgcgcatc gctggtggga tagatcgaga agtacttgcg aatctcttca
     1261 tcgttcatcc aggtggagta gaggtggttg tcctgcgccg gttccagcgg acgcaggtag
     1321 aggcgctcgc caatcaagaa cgggttttgc agatgagtcg tatcttctgt cacgggtaaa
     1381 gctctctatt tgaatcgttt ggcggaatga taaagggtct gtgtccccct gcttcatctt
     1441 cgccttcctg cacatcgggg gtgtgcctgc ggcgcgaacc aggattgcgc tcatccaggt
     1501 gatgcggggg cggttccggc gctgcttcct catcgcgccc gttctggttt tgctcctggt
     1561 cttcatctgc gggggctgcc agtagggccg attcgatgac gtgatccatt gtttcgacgg
     1621 tgatgaaggt catgtgctgg cgaattttcg cggggatctc ggccaggtct tttttgttgt
     1681 ccgccgggat cagcagttga aagatattgg cggcgtgcgc ggccagcgtt ttttctttga
     1741 ggccgccgat ggccagcacg cgcccgcgca gggtgatttc gccggtcatg gcgaggtcgc
     1801 cgcgcacggg acggtgggtt agcgcggaga tcagcgcggt ggcgatggcg atgcctgccg
     1861 atgggccttc tttgggcaga gcattctccg gcatgtgaat gtgcatgtcg gtcgagtcct
     1921 gaaagttcgg gtcaagctcc aggtctttgg cgcgcgagcg gatgtaggag agcgcggcga
     1981 aggccgactc gcgcatgact tcgccgagtt gtcccgtgat aagcacctcg cccttgccgg
     2041 gcatcgtgac gacctcgacg ggcagcacca tgccgccggc atccgtgacg gccaggccga
     2101 gagcaacgcc gacctggaca gtgtggttga tgggcgattc caggtaacgg ggcgcgccga
     2161 gatagttttc caggttgtgc gtcgtgacgc ggatatgcgt gtcgggcttt tcgaagatac
     2221 ggcgcgctga cttgcggcag atggtggtca attgccgttc gagggcgcgc acaccggcct
     2281 cgcgggtata gtcggtgacg atgtgctgca tgacggcctg ggggattttc aactgctccg
     2341 gcgcgatgcc atgttcggcc agcaccctcg gcagcagatg gcgcgagccg atgttgatct
     2401 tttcttcgag gatatagccg ggcaggtcga tcacgtccat gcggtcgaga agcgggcgcg
     2461 gaacctggta gcgcacgttg ccggtacaga tgaagaagac ttccgagaga tcgaagggaa
     2521 cttccaggta atggtctaca aagacattgt tctgggcgga gtcaagcact tcgagaaggg
     2581 cggcagctgg atcgccccgg tattcggagg agagcttatc gacctcatcg agcaggaaca
     2641 cggggttgcg cacgccgacg gttttcatgg attggatgat gcgaccgggc agcgcgccga
     2701 cgtaggtgcg gcgatggccg cgaatctcgg cttcgtcgct gacgccgccc agggcgatac
     2761 gcgcgaattt gcgtcccaac gcgcgcgcga tagattggcc cagggaggtt ttgcccacgc
     2821 ccggcggacc gatgaagcag aggatctgtc cctgactctc ctggcgcacg acccctgcgc
     2881 ggctggcgcg gcgctggcgc agttggcgga ccgcgagaaa ttcgagaata cgttctttga
     2941 tgccttctaa gccgtagtgg tcttcctcaa gaatgcggcg cgtcgtctcg atatcgaagt
     3001 gatcgtccgt tcgttcgctc cagggcagcg ccagaatcca gtcgatgtac gaacggatgg
     3061 aggcgatgtc ggctgattgt ggcggagtgc gttccaggcg gtcgatctcc ttgcgcgttt
     3121 tgtttgtcac atccaggggc ataccctgcg cggccagttc gttcacacgg tcgcgcagct
     3181 cgttcgcctc gctcgcgccc tccataccca gttcttcctg gatagcgcgc aggtgttcgc
     3241 gcaggtaaaa ctctttttgc gagcgatcga cctgagagcg cacgcgggtg cggatggtcg
     3301 attcgagttc aaggatctcg atctcgttgc ccatcaggac gcagattttc tccaggcgcg
     3361 ccaggggatc gagcatctca agcagttctt gctgctggtg aaagtcggtc acgacgaagg
     3421 tggccagcgt atcggagagc cgcgcggcgg ttttctgcga gacgatggag gtgatgtctt
     3481 cggcggtata tttgcgattg agctgagcgt agcgctcaaa caggttcgtg gcgtggcgca
     3541 cgacggcgtc ggtcatcgac ccatgcggct gttcttcgat cagtggctcc acgatcacgc
     3601 gcaggaaggg ttcctgttcg acgaattctt caatctgcac acgccgcagt ccctggagca
     3661 gtatctggaa gctgccgtcc tgctggcgat gcatggtggt gacttcgacc agtgtgccga
     3721 ccgagtagat gtcttttggt tggggatcgt cgatttcggc ttcctgctgg gcagcggtaa
     3781 tcaggaggtg ttcgggctgc atcatggcct cttcgacggc gcgaatcaac ttctcgcgca
     3841 tcatcgagag ggtcgtgcgc gtgcggggaa acacaaccat gtctctcaac gccacaagag
     3901 ggtatggcga gcgctgatcc atcagcgtat ctgggtgttc agtatcgttc acattcccca
     3961 ccttcgtagg gacgcggttc atcgcgtccg cgccattgcg atatggcgct ctacccgcat
     4021 cggacaatct tcgcttggcg tgtaggggcg caattcattg cgccctcgtc cctggtcaac
     4081 cgcc
//
LOCUS       DASZAK010000220         1714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_360960, whole genome shotgun sequence.
ACCESSION   DASZAK010000220 DASZAK010000000
VERSION     DASZAK010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360960"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..576
                     /gene="sigZ"
                     /locus_tag="VGT44_03735"
     CDS             22..576
                     /gene="sigZ"
                     /locus_tag="VGT44_03735"
                     /inference="COORDINATES: protein motif:HMM:NF007215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigZ"
                     /protein_id="HEV2579942.1"
                     /translation="MLLTTEHVWQEFNVGLKQFILKRIPDEQSADDILQEVFLKIHTR
                     IDTLREEEKLPGWVYQIARNAVYDYYREQKPDAGLPDIPYLPDDPHDDVVAELAPCIR
                     AMVDSLPAHYRQALILTEYEGMSQKALSEQLGLSFSGAKSRVQRARQQIKQMLLDCCH
                     FQFDTYGKIIDYQPRCACCATSAC"
     gene            complement(560..1423)
                     /gene="arsM"
                     /locus_tag="VGT44_03740"
     CDS             complement(560..1423)
                     /gene="arsM"
                     /locus_tag="VGT44_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEV2579943.1"
                     /translation="MTTVPTNDEQLRDAVRERYGRRALVVTNAESASASAGCCDSGCC
                     GGTAGQNGDAITTDLYSSAELGALPVAAALASLGCGNPTALAELKAGEKVLDLGSGGG
                     IDVLLSARRVGPTGFAYGLDMTDEMLALAERNRQEAGVENVRFLKGYIEDIPLPDGAV
                     DVVISNCVINLSADKGKVLREAYRVLAPGGRFAVSDIVFQGTIPAAIRGDLEAWAGCI
                     AGALEEQVYRDLLQDAGFSQVEVEVTRRYGLDDVANSGGAASLTALSESERATVDGKF
                     VSAFIRASKPM"
BASE COUNT          360 a          508 c          470 g          376 t
ORIGIN      
        1 agtaagtgga gggtatgacc gatgttactc acaacggaac atgtctggca ggaatttaat
       61 gttggactca aacaatttat cctcaagcgc attcctgatg agcagagcgc cgatgatatc
      121 ttgcaggagg tgttcctcaa aatacatact cgcatcgata cgctgcgtga agaagagaag
      181 ctgccgggct gggtctatca gattgctcgc aatgcagtct atgattatta ccgcgagcaa
      241 aagcctgatg ccgggttgcc ggacatcccc tacctgcccg atgatccgca cgatgatgta
      301 gtagcggaac tcgcgccgtg cattcgcgct atggtcgata gcctgcccgc gcattaccgg
      361 caggcgctca ttttgaccga gtacgagggt atgtcgcaaa aggccttgag cgaacaattg
      421 ggtctctcgt tttcgggcgc gaaatcgcgt gtgcagcgcg ccaggcagca aatcaagcag
      481 atgctgctcg attgctgcca tttccagttc gacacctatg gcaagatcat tgattaccag
      541 ccaaggtgcg cctgctgcgc tacatcggct tgctagcccg gataaacgcg ctgacaaact
      601 tcccgtccac tgttgcgcgc tcgctctccg agagtgccgt gagcgaagct gcaccgccgc
      661 tgttggcgac atcatccaat ccatagcgcc gcgtcacttc gacttcgacc tggctgaagc
      721 ccgcgtcttg cagcagatcg cgatagacct gctcctccaa tgccccggcg atgcagcccg
      781 cccaggcttc caggtcgcca cgaatagctg cggggatcgt tccctggaaa acgatgtccg
      841 agacggcgaa gcgaccaccg ggcgcgagga cgcggtaggc ttcgcgcaag accttgccct
      901 tatcggctga gagattgatg acgcagttgg agatcaccac gtccacagcg ccgtctggca
      961 gcgggatgtc ttcgatgtag cccttcaaaa agcgcacgtt ttcaactccc gcctcctgcc
     1021 ggttgcgctc ggccagcgcc agcatctcgt cggtcatatc cagcccgtag gcgaagcccg
     1081 tcgggccaac gcgccgcgcc gagagcagca cgtcgatgcc gccgccgctg cccaggtcca
     1141 gcaccttctc gcccgccttc aattccgcca gcgcggtcgg gttgccgcag ccgagcgagg
     1201 ccagcgccgc cgcgacgggc aatgcgccca gctcggcgct gctgtagagg tcggtggtga
     1261 tagcgtcgcc gttttgcccg gctgttccgc cgcaacagcc actatcgcaa caccccgcgg
     1321 atgcgcttgc cgattcggcg tttgtgacca ccagcgctct gcggccataa cgttcgcgca
     1381 ccgcgtcgcg cagttgttca tcatttgtcg gtacagtagt catagttgtc gtcctttctt
     1441 tcgtttcgtg aacgtcatca cgattactat acaatagacg aactttttga aaaagagacg
     1501 caatgaacgc aatgaaattt tccttgactt ttggccgatc actgtgtata cttctagcaa
     1561 tttgaaaggt ttccctttgc aaaacatgct ggaaaaggct gtctatcgtc atgatcttga
     1621 tataccggga ctcagggagt gcgaaattaa ggaggcggaa ttgcctctca ttttctgtta
     1681 aatggcccgg tatcgtacca ccggaaaagg attc
//
LOCUS       DASZAK010000221         2710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_502849, whole genome shotgun sequence.
ACCESSION   DASZAK010000221 DASZAK010000000
VERSION     DASZAK010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502849"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VGT44_03745"
     CDS             <1..413
                     /locus_tag="VGT44_03745"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2579944.1"
                     /translation="KTMANIVPYITFAKNGQEAITFYKGVFGGDAEVQFEGEGVIHLD
                     FQAGGIHFMGSDLQSDQAGLERGHGSSLVLNCDSEEQLRDFYARLVDAGREVFAPTDS
                     GWGAIIAHCTDQFGVTWLMNYDKPQSEQSGMSRA"
     gene            complement(704..838)
                     /locus_tag="VGT44_03750"
     CDS             complement(704..838)
                     /locus_tag="VGT44_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579945.1"
                     /translation="MSWLVIVLVLVPVKVRVSFTHPEVEPVEARTDHMVEKLSYRAVM
                     "
     gene            complement(861..1997)
                     /locus_tag="VGT44_03755"
     CDS             complement(861..1997)
                     /locus_tag="VGT44_03755"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV2579946.1"
                     /translation="MKRSVSVSRVVSHPSTLAVFSGLLGALIGFLLNRAAGGNTPQVI
                     WIALIFAILFSLSLTAWQVYTQEQTGKQWMTMLQEMVFQTYFLTLLADKPDISQLAQQ
                     RLGQVLKTLNGEQQASMLQFFSHNGLPATFIGDALRGSTGLVGADLQRIELPQIQLAH
                     ANLSRVNFREANLREANLGDATLYRADLSGANLSQANLTGADLRGVNLREANLTGADL
                     SDARMAMEKAGRDASAVPIQVGAFRYAALRANLSHALLSHARLREADLVGADLGGAKL
                     QGVDLTKANISYANLQGADLSGATLAEAACLGANLQGANLSGATLTEAALISANLQGA
                     DLSGATLTWAILDEADLRAAKVTEEQLQTVVSGKNVKREPRTEQ"
     gene            complement(2068..>2710)
                     /locus_tag="VGT44_03760"
     CDS             complement(2068..>2710)
                     /locus_tag="VGT44_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEV2579947.1"
                     /translation="AASGARVLPTDVLHQMKEPTAELRASSLGDAIGLGWFLRDVDGV
                     PTVGHAGSANGQFAELLIVPERGFAVMSMSNAGPGGIPFNQEVVSFALENYLGFVDRS
                     PEPIPFDAARAQELAGTYANAMMTCTIDAVAARLRLELRIKPEIRAAADKEPPPDPSP
                     CDFGLLPGNKDEYIITSGEFKGQRGFFTRDTSGAVVGVDLAGRLFSRVPIASE"
BASE COUNT          585 a          804 c          765 g          556 t
ORIGIN      
        1 gaaagactat ggcaaacatt gtaccgtaca tcactttcgc gaagaatggc caggaggcga
       61 tcacgtttta caagggggtg ttcggtggtg atgccgaggt gcagtttgag ggcgagggcg
      121 tgatccacct ggacttccag gccggcggta ttcactttat gggcagcgac ctccaaagcg
      181 atcaggctgg gttggagcgc ggtcacggtt ccagtctggt gctcaattgt gacagcgagg
      241 aacagctgcg agatttctac gccaggctgg tggacgctgg ccgtgaggtg tttgcgccga
      301 ccgacagcgg ctggggcgcc atcatcgccc actgcaccga ccagttcggt gtcacctggc
      361 tgatgaacta cgacaagccg cagagcgagc agtcggggat gtcacgcgca tgagtggcca
      421 atgaccgcat ctactcgtgt tcgtcaggaa tgctggcctt atcatcgcgc gcctcgcgag
      481 agaaacagca cgattgaaag gagttcttaa gatgaacgaa cccatcacca gttctaatcc
      541 ccatgagtgg gagcacacca tcagaagcaa ggagcagaca gctatgacag aacaggcatc
      601 tcccctcgtc actttctaca aacacggctg ggagcactat caacaagccc tcgtcaaaac
      661 catcgcatcg ctttcatctg aacaacttac ccttcctgtt gcgctacatt accgctcgat
      721 aggagagctt ctcgaccata tgatcggtgc gcgcttcaac tggttccacc tctggatggg
      781 tgaaggagac cctgaccttg actggaacga ggacgaggac aatgacgagc cagctcaacc
      841 ggtaccctca ccttctaatc ctactgctcc gttcgaggct ctcgcttcac gttttttcca
      901 gagacgaccg tctgcaactg ctcttcagtc acttttgccg cacgcaaatc agcctcgtcg
      961 aggatcgccc aggtgagcgt cgccccgctc agatcagctc cctgcaaatt cgcgctaatc
     1021 aaagcagcct cagtcagcgt cgccccactc agattagctc cctgcaaatt cgcgccaagg
     1081 caagcagcct cagccagcgt cgccccgctt agatcagctc cctgcaaatt cgcataactg
     1141 atattcgctt tggtgagatc aactccctgc aacttcgccc cacccagatc agcgccaacg
     1201 agatcggctt ctcgcagcct ggcatgagag agaagggcat gcgacaggtt tgctctaaga
     1261 gctgcatagc gaaaagctcc tacctgaatc ggaacggcgg aagcgtcacg acctgctttt
     1321 tccatcgcca tgcgagcatc gctcagatca gcgccagtga gattggcttc ccgtaaattg
     1381 acccctctca ggtcagctcc tgtgaggttc gcctgggata gattggcgcc agagaggtcg
     1441 gcacgataca atgtcgcatc gccgagattg gcttccctga gattggcttc tctgaaattc
     1501 acgcgactca gattcgcatg tgctaactgg atctgaggaa gttcaatccg ctgcaggtct
     1561 gctccgacga gaccagtgct tcctcgcaag gcatcgccga taaacgtcgc aggcaggccg
     1621 ttgtgactga aaaactgcaa catgctggcc tgctgctcgc catttaaggt tttcaacacc
     1681 tggcccagcc gttgttgtgc cagctgtgag atgtctggct tgtcggccag caacgtgagg
     1741 aagtaggtct ggaagaccat ctcctgaagc atcgtcatcc attgcttccc cgtctgctcc
     1801 tgcgtgtaca cttgccaggc cgtgagactc agactgaaca aaatggcgaa gatgagtgca
     1861 atccagatca cttgaggggt atttcctcca gcggcacgat tgaggagaaa gccgatgagc
     1921 gcgccaagta atccagagaa cacagccagg gtcgagggat gagagacgac ccggctcaca
     1981 ctgacagaac gtttcatgca cgatgactcc tttccgactt ccgaggtttg ctctcagtat
     2041 atcctctcac agctcgggaa accggactta ctcggaggcg atcggaaccc ggctgaataa
     2101 ccgaccggcg aggtctactc ctacgaccgc gccactcgtg tcgcgggtga agaagccgcg
     2161 ctgccccttg aattcgccgc tggtgatgat gtactcgtct ttgttgccgg gcagcaggcc
     2221 gaaatcgcac ggcgaggggt ccgggggcgg ttctttgtcg gccgccgcac ggatctctgg
     2281 tttgatccga agctcaagcc tgagcctggc cgcaaccgcg tcaatggtgc atgtcatcat
     2341 cgcgttcgcg taggtcccgg caagctcctg ggcccgcgcc gcatcaaacg ggatgggctc
     2401 tgggctgcgg tcgacgaatc ccaggtagtt ctcaagcgcg aagcttacga cctcctgatt
     2461 gaaggggata ccacctgggc cggcgttgga catcgacatg acagcgaagc cgcgctcagg
     2521 gacgatgagc agctcggcga actggccatt ggctgacccg gcgtgcccga cggtgggcac
     2581 gccgtctaca tcgcgcagga accagccgag gccaatggcg tcgcccaggc tgctagctcg
     2641 caactcggcc gtgggctcct tcatctgatg tagcacgtcg gtggggagca ctcgggcacc
     2701 gcttgctgcg
//
LOCUS       DASZAK010000222         4323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_573768, whole genome shotgun sequence.
ACCESSION   DASZAK010000222 DASZAK010000000
VERSION     DASZAK010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573768"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..804
                     /locus_tag="VGT44_03765"
     CDS             244..804
                     /locus_tag="VGT44_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2579948.1"
                     /translation="MDQVEKERLTLEKELSQFLSQSFLELDEGERQLMKRFGLTLTQY
                     WALVHLKNEDGRSLSELADLLICDKSNVTSIVDKLEESGLAERKRGKAGDRRYIRVIL
                     TPEGQKVRSVLIAAQDHLLKLRLRSLNMENLQRLREPLQQLAQTLQAQFKHDEVPSMI
                     DNSVEYMHAEQEIAAILSRVTRNAIW"
     gene            1255..1968
                     /locus_tag="VGT44_03770"
     CDS             1255..1968
                     /locus_tag="VGT44_03770"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEV2579949.1"
                     /translation="MSSVKNAWQQEQATQSYPLITRLLLWAGAVGPLLFVAVLLIEGA
                     TRPGYSAWRTFGSLLSLGDQGWMQITNFIVCGVLALGFAIGLRQVLHSGKGAVWGPIL
                     LSLFGLLLIIAGIFVTDPGQGYPVGVTPPVSPTGHGIIHALCGLAIFITLPIAAFAMI
                     RRFAGDPTWRDWAFYSLLTGLIMIVSFFLSQLPGANALAGLLQRVGIIAGWVWVSLFA
                     IALLRRGRAPEVIASSIPR"
     gene            complement(2154..>4323)
                     /locus_tag="VGT44_03775"
     CDS             complement(2154..>4323)
                     /locus_tag="VGT44_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV2579950.1"
                     /translation="AVNTIGGTAPNNDQWTNSSWASSNNSILGTAQNTSSDVWFTGGN
                     GIIGEVFYPTADTPNTTDLQFLVGDSSHTWVNQEQNDTISKVHLYDNHSLAWVVTNTA
                     RNGDYQITKIIFADPTRNSLIQQTTFKALQGHLADYQLYVYYNPAIHNKGDNNNSSTK
                     TYQGRVILVSTSGAGKYASALAASLPYQKGMTSSGFLGQSDGWTDLAGKSACGSATCP
                     DYTMNYTYSAAADGNTVQTGLLDLSNGGMLDLSSATSLTFNLVLSFGQSSGGGDATTG
                     AEQALLGSLNDRSNMLSTYVAQWNSFDDSLRAPPAVGSTAAIQQARQQEYYLAANVLK
                     ASQDKQSGTFVAGLGTPWGESNGDQDTGGYHLVWERDMYEFSSALIVAGDTADPKRAL
                     LWAFTKQQQPGGLFPQNSYVDGTPYFGGVQMDEQAFPIMLAWKLGVTDRQDYLQHIKP
                     AADLLVKSGGITGQERWEENGGYSPSTIATMISGLVSAAAIARINNDVASQQRYLSTA
                     DNYRKMVIAWTYTTTGPLGNGHYFERIDGNGNPNDTDMIQLTNGGGTFDERSVVDMGF
                     LELVRQGIYPANSPYITSSLPVVDATLEETINGNSYWHRYNHDGYGEHANGSDYDGTG
                     VGQLWPILSGERGIYEVAAGQNADQYLTAMMAAANASGLIPEQVWGNNAPARYTPGTP
                     TKSMNPLNWAMGEYITLLFSVSEHAIADVVSITAQRYRGL"
BASE COUNT          897 a         1149 c         1202 g         1075 t
ORIGIN      
        1 tgcttcacgt tgctcgtcaa ggggtcttcc catgtcgggt tccactccat ctctcgttcc
       61 tcctctgttg attctgggtc ttcccttgat tctatcacct ctttcccatt tatgagtgga
      121 tgtcaggcat gagtcttgac attccgctaa ttgcgagata tgctggtgtc agcaatacag
      181 ttgaatgcgc aacagtatga aagagattca tgttgaatgc gcaacagtac gaaagagaac
      241 cacatggacc aggttgaaaa agaacgtctg acactagaaa aggagctttc tcaatttctt
      301 tcacagtcct ttcttgaact tgatgaaggt gaacgccaat tgatgaagcg cttcgggtta
      361 actctcaccc agtactgggc gctggttcat ctcaaaaatg aagacggtcg ctctctcagc
      421 gaactggctg atttgctgat ttgcgataag agtaatgtaa caagtattgt tgacaaattg
      481 gaagaaagcg gactggcaga acgcaagcga gggaaggcgg gagaccggcg ttatatccgc
      541 gtgattctca ctcctgaggg acagaaagta cgcagcgtat tgattgccgc acaagatcat
      601 ttactcaagt tgcgtttacg atcgctgaat atggagaacc tgcagcgact ccgcgaaccc
      661 ctacaacaac tggcgcaaac gctccaggct caatttaagc atgatgaagt tccctccatg
      721 attgataact ccgtcgagta tatgcacgca gaacaagaga tagctgcaat tttatcccgc
      781 gttacgcgga atgcaatctg gtaaatagcg cgaacgttgc actcttttta gggtcttgct
      841 cactttcggt agtttgagta ttgtagagtt atgaccgcac agcaagagat cgctccagaa
      901 gccaagaaag cttttcactc ttgtctgttg atgggcttaa acaccgaatg tgagcaggac
      961 ccctttttag cgcgaaatgt tggaaatcgg cgcgaatggt gggaatcaga gtgaagccgg
     1021 ctgttcgtcc cgctctacac gtggtcttct tctcctaaga cttttaaatt cgtgctggat
     1081 atcgaagact cgactatgat cacgcgcggt aggtatgcaa agaacaggac ttcagtaggg
     1141 gacacatcgc gccttcagac gggttttatc tcacttcaat tatgttacgc ttgcggtatc
     1201 agattttcta ctgaaaagca agaaacagta gttttattca aggagatttc tctcatgagt
     1261 tctgtcaaaa atgcctggca gcaagagcaa gcaacacaga gttatccttt gatcaccagg
     1321 cttctgctct gggctggcgc ggttgggcca ctactctttg tggccgtgct cttgatcgaa
     1381 ggagccacgc gccccggtta tagcgcctgg cgtaccttcg gcagtttatt gagtctgggc
     1441 gaccagggat ggatgcaaat caccaatttc attgtctgcg gcgttctcgc cctcggcttt
     1501 gccattgggc tgcggcaggt gctccattca ggtaaagggg cggtctgggg accaattctg
     1561 ctcagtctct tcggtctgct gttgatcatc gccggcatct ttgtcactga tccgggccag
     1621 ggctaccctg tgggtgtcac accgccggtc tctccaacgg ggcatggcat cattcatgcc
     1681 ctttgtggcc tggcgatctt cattacattg cccatcgccg cctttgccat gatcaggcgc
     1741 tttgcgggcg atccgacgtg gcgcgactgg gccttctact ccctgctcac cggtctcatc
     1801 atgatcgtgt cgtttttcct ctcccaactg cctggcgcca acgcgctcgc cggcctgctc
     1861 cagcgtgttg gaattatcgc aggctgggtc tgggtctcgc tgttcgctat tgcactcttg
     1921 cgaagagggc gcgcgcccga agtgatagca agctccatcc ctcgctaaat ctcgccggga
     1981 acgcgactcg cctgaagtac agctaagatc ctgacgaata tgtgcaggat ccaaaaatcg
     2041 tgcggattta acccaattta ccacaatcgc aaattttgcc gaatccagat catctgctta
     2101 gacgaaatac ctcggccacc tgcgcaggga agacgagagc gaccgcaagg ggtttatagt
     2161 cccctgtagc gctgagcagt gatcgagacg acatccgcaa ttgcatgctc gctcaccgaa
     2221 aagagcagcg tgatgtactc acccatcgcc cagttgagcg ggttcatcga tttcgtcggc
     2281 gttcccggcg tatatcttgc cggggcgtta ttgccccaga cctgctcagg gatcaagcct
     2341 gaagcgttag cagccgccat catcgccgtc aggtactggt cggcattctg accggctgcg
     2401 acttcgtaga tgccgcgctc tcccgaaaga atcggccaga gctggcctac gcccgttcca
     2461 tcgtagtcgg aaccgttggc atgttcgccg tagccgtcgt ggttgtagcg atgccagtag
     2521 gaattgccgt tgatcgtttc ttcaagcgtg gcatcaacga ctggcaggga cgatgtgata
     2581 tacggcgagt tcgccgggta gataccctgg cgcaccagtt cgaggaagcc catgtcaacg
     2641 acggaacgct cgtcgaaggt gccgcctcca ttggtgagct ggatcatatc ggtatcgttc
     2701 ggattgccgt tgccatcgat gcgctcgaaa tagtggccgt tgcctagagg accggtggtg
     2761 gtataggtcc atgcaatcac cattttgcgg tagttgtcag cggtgctgag atagcgctgc
     2821 tggctggcaa cgtcgttgtt gatacgggcg atcgcggcgg cgctcaccag cccgctaatc
     2881 atggtcgcga tggttgacgg gctgtaaccg ccgttctctt cccagcgttc ctgtcccgtt
     2941 atgccgcccg atttgacgag gagatcggcg gcgggtttga tatgctggag ataatcctgc
     3001 ctatcggtga cgcccagttt ccacgccagc atgatcggga acgcctgctc atccatctgc
     3061 acgccaccga aatatggagt gccatcaacg tagctgtttt gagggaacag acctcccggc
     3121 tgctgctgct ttgtaaatgc ccagagcagc gcccgtttcg gatcggcagt atctcctgcc
     3181 acgatcagcg cgctggagaa ctcgtacata tcgcgctccc agacgagatg atagccgccc
     3241 gtgtcctggt cgccgttgct ctcgccccag ggcgtcccca atccggcgac gaacgtaccc
     3301 gattgcttat cctgcgacgc cttcaacaca ttcgccgcca ggtagtattc ttgctgccgc
     3361 gcttgctgga tggccgctgt gctgcctact gccggtggtg cgcgcaggct gtcatcgaag
     3421 ctgttccact gcgcaacgta ggttgacagc atatttgacc ggtcgttgag cgatcccagc
     3481 aacgcctgtt ccgcgccggt cgtggcatcg ccgccgccac tgctctgccc gaaagagaga
     3541 acaagattga aggtgagcga ggtcgcggaa gagagatcaa gcatgccgcc gttggagaga
     3601 tcgagcaaac cggtctgcac ggtattcccg tcggcggccg cgctgtaggt gtagttcatg
     3661 gtgtagtcgg ggcaggtagc gctgccgcaa gcagacttgc ctgcaagatc tgtccagccg
     3721 tcgctctgcc caagaaagcc ggaactggtc atgcctttct gatagggaag agatgccgcg
     3781 agggcgctgg cgtacttccc ggcgccgctg gtgctgacca gtatgactct gccttgatat
     3841 gtttttgtag agctgttgtt gttatcgcct ttgttatgta tggccggatt gtaatagaca
     3901 tagagttgat aatcggccag atgcccctgc aaggctttga acgtggtctg ctggatgaga
     3961 gagttgcgcg tcggatcggc aaagatgatt ttcgtgatct ggtaatcgcc atttctggct
     4021 gtgttcgtga ccacccacgc cagcgagtga ttgtcgtaca ggtggacttt tgagatggta
     4081 tcgttttgct cctggttgac ccaggtgtga ctgctatctc cgacgagaaa ttgcaggtcg
     4141 gtcgtgttcg gagtgtcggc agtcgggtaa aatacttctc cgatgatgcc gttgccgccg
     4201 gtaaaccaga catcgctgct tgtattttgt gccgttccca ggatggaatt gttggaagaa
     4261 gcccagctcg agttcgtcca ctggtcgttg ttgggcgcag tgccaccaat cgtgttcacg
     4321 gcg
//
LOCUS       DASZAK010000223         1586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_432099, whole genome shotgun sequence.
ACCESSION   DASZAK010000223 DASZAK010000000
VERSION     DASZAK010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432099"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..>1586)
                     /locus_tag="VGT44_03780"
     CDS             complement(213..>1586)
                     /locus_tag="VGT44_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV2579951.1"
                     /translation="GPTEWRFSINNQQSTIPLTTSATSTNSSTLRKAGLFYLVFVMFS
                     YTTGGPFGLEDMVTTSGPGITLLYLLILPFFWCIPVSLVSAELTTAMPVEGGFYRWTR
                     AAFGDFWGFLAGWWNWSASFLLGGAYAVLFADYLVYVFPKMTWWEHWLISLGLIALLT
                     WVNVRGIQMVGQVATALEIFIFVPVMIMVAIGLAHWHQNPFHPWVVPHQATFKVFGVG
                     LALGLWLYSGYEQLSTVAEEVENPQKAYPRALALVVPLSMAAYFLPTVAGLASAGNWQ
                     QWHTGFFSDAARIIGGPWLGTWMTLAAVIGNVALLNSTILTTTRMPFAMAEDGYLPDA
                     LTRKHSRYGTPWLAIVASAVIYALLAWQSLGQLISVYIWLRSATTVLTVLSGWKLRRT
                     RPELARSFIVPGGQAGLLYVVAAPVLMAIVALLGGDRFATIGGVIAIVLGPVIYALIR
                     LTASRTS"
BASE COUNT          349 a          486 c          487 g          264 t
ORIGIN      
        1 ccggtccgta tctcaatctc gtcgccctct ttcaattcaa gcgcgtcgac cacggcggcc
       61 gggagccgga tcgcaagact gtttccccac ttcgctacgc gcattggttt ctcctcggat
      121 atacatttca attgtatatc aatgatgctt ctggagcaac ttcgtcgaag cgggggcagg
      181 atgccctcgc gacagccgcc ctgacgccgg cgctacgatg tgcggctggc ggtgaggcga
      241 atgagagcgt agataaccgg ccccagcacg atcgcgatca cgccgccgat ggtggcgaag
      301 cggtcgccgc ccagcagggc tacgatggcc atcagcaccg gagccgccac tacgtagagc
      361 aatcccgcct ggccgccggg cacgatgaat gaacgcgcca gttcgggccg cgtgcgccgt
      421 agtttccatc ctgaaagcac ggtcagcacg gtggtcgccg agcgcagcca gatatagacc
      481 gagatcagtt gcccgaggct ctgccaggcc agcagagcgt agatcacggc cgaggcgacg
      541 atcgccagcc acggtgttcc ataacgcgaa tgcttgcggg tcagagcatc gggcagatat
      601 ccgtcttcgg ccatggcgaa cggcatgcgc gtggtggtca ggatcgtgct gttcagcagc
      661 gcgacatttc cgatgacggc ggccagcgtc atccaggtac ccagccacgg gcctccgatg
      721 atcctggcgg catcggaaaa aaatcccgtg tgccactgct gccagtttcc tgccgaggcc
      781 agtcccgcaa cggtgggcag aaagtatgcc gccatcgaga gcggcaccac cagcgccagc
      841 gcccgcggat acgccttctg cgggttctcg acttcttccg ctacggtcga aagctgttcg
      901 tatcccgaat acagccacag ccccagggcg aggccgacgc cgaacacttt gaatgtcgcc
      961 tggtgcggca cgacccaggg atggaaagga ttctgatgcc agtgcgccag gccgatggcg
     1021 accatgatca tcaccgggac gaagatgaaa atctccagcg cagtcgcaac ctgccccacc
     1081 atctgaatgc cacgcacgtt aacccaggtc agcagcgcga tcaggccgag cgagatcagc
     1141 cagtgctccc accaggtcat cttggggaag acgtagacga gataatcggc gaacaaaacg
     1201 gcataggcgc cccccagcag gaacgaagct gaccagttcc accatccggc gaggaatccc
     1261 cagaagtcgc cgaaggcagc ccgggtccag cggtagaaac cgccttcgac gggcatggcg
     1321 gtcgtcaact ccgccgaaac cagcgagacc gggatacacc agaagaaagg cagaatcagc
     1381 aggtagagca gcgtgattcc cggcccggag gtggtgacca tatcttcgag gccgaatgga
     1441 cccccggtgg tgtaggagaa catcacgaag acgagataga acaacccggc cttgcggaga
     1501 gtcgaggagt tggtgctggt ggcggatgtg gtcaaaggaa ttgttgattg ctgattgttg
     1561 attgaaaacc tccactcggt tggtcc
//
LOCUS       DASZAK010000224         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_325472, whole genome shotgun sequence.
ACCESSION   DASZAK010000224 DASZAK010000000
VERSION     DASZAK010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325472"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VGT44_03785"
     CDS             <1..1460
                     /locus_tag="VGT44_03785"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV2579952.1"
                     /translation="QDGSPEQESLLEELRAEGLGAEEIALWTTQELTLQNVLSFKVSQ
                     GVEVKALVWECLPIFTHLSPQTAYEQLTAIGVSCILDDSAKGLMHHPVEALHQKISVI
                     DGTHAFVGGIDLLIDNDGDYDRWDMHRHHYSSDLRRNQQTRDPHPWHDAHAILEGTAV
                     GDVELNFRQRWNNVVERHKMDRALLIPERPMPAPKDSRSVVQVVRTIPEHTYDFDPDP
                     GIQGIAQVYVHALANAQRFVYLENQYFWLHAYFGLDIAAIGLDSPDMERNIRELGAAL
                     QRGATVSIILPDHPNVGRAFTDAGLERLRQEAPDAAAQGRLQVFCLATSMNLDGAELY
                     RPIYVHAKVAVIDDTFSTVGSGNLNNRGMRDDTEMNVAVLDSRLAHGLRMMLVAEHLG
                     LYDEDELLMISRHLGNQRQRAEREQRAIEMLAVLEEQFGNPRVALEMMVANARENMRR
                     YQAHQPLVGHLLPYFTYAEAQRAELRCNEDSGWLE"
BASE COUNT          338 a          452 c          454 g          285 t
ORIGIN      
        1 ggcaggatgg tagtccagag caggaatcgc tgctagagga actgcgcgcc gaggggctgg
       61 gagcagagga aatcgcgtta tggacaaccc aggaacttac gttgcaaaat gtgctgagct
      121 tcaaggtcag tcagggcgtc gaggtgaagg cgctggtctg ggaatgctta ccgatcttca
      181 cgcatctcag cccgcaaaca gcttacgagc agttaacggc gatcggcgtt tcatgtatcc
      241 tcgacgacag cgcgaaaggg ctgatgcacc atcccgtcga ggcgctgcat cagaagatct
      301 cggtcatcga tgggacgcat gccttcgtgg gcggcatcga tctgctgatt gataacgacg
      361 gggattatga ccgttgggat atgcaccggc accattattc ctccgacctg cgccgcaacc
      421 agcagactag agatccgcac ccctggcacg atgcccatgc catcctggaa ggaacggcag
      481 taggtgacgt ggaactgaat tttcgccagc gctggaacaa cgtcgtcgag cgccataaga
      541 tggaccgcgc cctcctgatt ccagaacggc cgatgcccgc gccgaaagac agcagaagcg
      601 tcgtgcaggt ggtacgtacc attcccgaac atacctacga tttcgatccc gatcccggca
      661 tccagggcat cgcgcaggtc tacgtacacg ccctcgccaa tgcgcagcgc tttgtctacc
      721 tggagaacca gtacttctgg ctacacgcct actttggact ggatatcgcc gcgatcggtc
      781 tagacagccc cgatatggaa cgtaacattc gcgaacttgg cgcagcgcta caacgaggcg
      841 caaccgtctc gatcatcttg cccgatcacc ccaatgtggg ccgcgccttc accgatgccg
      901 ggctggagcg gctgcgccag gaagcgcccg acgcggcggc gcagggtcgc ctccaggtgt
      961 tttgcctggc aacctcgatg aacctcgacg gcgctgaact gtaccgccct atctacgtcc
     1021 acgcgaaggt tgcggtgatc gacgacacct tctcgaccgt tggttctggc aatctcaaca
     1081 atcgcgggat gcgcgacgat accgagatga acgtcgccgt cctcgactcc cggctggcac
     1141 atggtctgcg gatgatgctc gtggcggaac acctgggatt gtatgatgag gatgagctgc
     1201 tcatgatctc ccgccacctg ggcaatcagc gccagcgagc ggaacgggag cagcgcgcaa
     1261 tcgaaatgct ggctgtcctg gaagagcaat ttggcaatcc ccgggttgcc ctggaaatga
     1321 tggttgcaaa tgccagggag aacatgcggc gttaccaggc gcatcaaccc ctggtcggcc
     1381 acctgctgcc atacttcact tacgcggagg cacaacgcgc agagctgcgc tgcaacgaag
     1441 actcaggatg gctagaatag aaggattgac acaccaataa acggggcatc ctggccggcc
     1501 aggatgcccc ggttattggt gtgtcaatc
//
LOCUS       DASZAK010000225         4168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_680463, whole genome shotgun sequence.
ACCESSION   DASZAK010000225 DASZAK010000000
VERSION     DASZAK010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680463"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..743)
                     /gene="istA"
                     /locus_tag="VGT44_03790"
     CDS             complement(<1..743)
                     /gene="istA"
                     /locus_tag="VGT44_03790"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEV2579953.1"
                     /translation="MREATRNEIVRLHYAGTSQRAIARLLGIHRKSVFRVLRNHQDRR
                     AGAEEKQRAHRPSLLDPYADQIAKLLERYPNLTAVWLHEELSKRGFEGRYTIVRERLR
                     ALRPRAPKAPVQRFETAPGVQAQMDYSTYEIPFTAEGRRRVHAFSYILSYSRRQYLRF
                     VESQDFTTTIREHARAFAYFQGAAATCLYDNMKVVVTGYDGDQPIYNTRFLAFATHYG
                     FQPWACRPYRPQTKGKVERPFQYVATNLL"
     gene            complement(740..1657)
                     /locus_tag="VGT44_03795"
     CDS             complement(740..1657)
                     /locus_tag="VGT44_03795"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEV2579954.1"
                     /translation="MLRWASQDLLHLQHQEIGEHYGRYRLHLPDAERAMARSLKRYGQ
                     LSPVVVCRRQERYELIDGFKRLGAARHLVGIESLSARLMEADERSAKAAIYGLNRAGG
                     RTRELEEAWIIHALVREDNMSQVEVAELLGRHKSWVCRRLALIERLDSKARDDLRVGL
                     LSPTSARQIVRLPQGNQAEVLDAARRETLSGAELAGVVDLWLGCGDRGQQQYILTHPR
                     EALSQAKGLSHAVRDPRLSEAGNHVWKRVGLLLDVLGRMEVWLAHHGRAGLTAEDRGL
                     LAPRFEKLSRDAGSVAALSLDLVNELKTV"
     gene            1714..1851
                     /locus_tag="VGT44_03800"
     CDS             1714..1851
                     /locus_tag="VGT44_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579955.1"
                     /translation="MSGLSVAPAVALLATTGARPPPASLSLPNAFHRAPARAGTEALE
                     R"
     gene            complement(3211..>4168)
                     /locus_tag="VGT44_03805"
     CDS             complement(3211..>4168)
                     /locus_tag="VGT44_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV2579956.1"
                     /translation="SFRLHLECVSHWEHIASRRGYRPSHPIWPPYFQVLTATQNLNSV
                     PFCSKNKIGLPKFVSTFGTPRTILRWLDQSHPHEAIGCYMRGAIYVRCSTTSKKKFGD
                     VSAYLQNPEAQVEPLVGLLASRGWELHRVYSDRMSGAKESRPGLQALMDDARRGMFGV
                     AVVWRFDRFARSVKQLVMALEEFRTLGIEFISHQEALDTSTPMGKAMFTIIAAMAELE
                     RSVIRERVMAGLEYAREHGTKSGRPVGRPKKIFDREEALRLRQRGMRIRAIARQLGIG
                     SGTVVRALKEFAGQTDHIPPPPARVPVLVMPKASSADLEQQP"
BASE COUNT          805 a         1280 c         1167 g          916 t
ORIGIN      
        1 ttgagcaggt tcgtggcgac gtattgaaaa ggtctctcca ctttaccttt cgtttggggg
       61 cgatatggcc ggcaggccca gggttggaaa ccataatggg tggcaaaggc cagaaagcgc
      121 gtgttgtaga tgggctggtc accgtcgtaa ccggtcacta cgaccttcat gttgtcgtat
      181 agacaggtgg ctgctgcgcc ctggaaataa gcgaaggcgc gagcatgttc gcggatcgtc
      241 gtggtgaagt cctgcgattc gacgaaccga agatactgcc ggcgcgaata actcagaatg
      301 taactgaaag catgcacccg gcgccggcct tccgcagtga atggaatttc gtaagtcgaa
      361 taatccatct gtgcctgaac acccggagct gtttcgaatc gctgcacagg tgctttcggc
      421 gctcgcggcc gtaacgcgcg cagccgttct cgcacgatgg tgtagcggcc ttcaaaaccg
      481 cgtttgctta gctcctcgtg cagccacacc gcagtcagat tggggtagcg ttccaggagt
      541 ttcgcaatct gatcggcgta agggtccagc aggctgggtc ggtgcgcccg ctgcttttcc
      601 tcggcgccag cccggcgatc ctggtggttc cggagcaccc ggaacacgct cttgcgatgg
      661 atgcccagca ggcgcgcgat ggctctttgc gaagttccgg cgtaatgcag acgtacgatc
      721 tcattgcgag tggcttccct catacggttt tcagctcatt cactagatcc aggctcagcg
      781 ccgcgactga cccagcatcg cgcgacagct tttcaaaacg tggcgccagg agcccgcgat
      841 cttccgcggt caggcctgcg cgaccatgat gcgccagcca cacttccatc cggcccagca
      901 catctagcag aagtcccacg cgcttccaaa cgtgattacc ggcttcgctc aaccgcggat
      961 cgcgcaccgc atgactcaag cctttagctt gcgatagtgc ttcccgggga tgcgtcagga
     1021 tgtactgttg ttggccgcgg tcgccgcatc ccagccacag atcgaccacg cctgccagtt
     1081 ccgcgcccga gagcgtctcg cggcgcgccg cgtcgagcac ttcggcctgg ttgccctgcg
     1141 gcaaccgtac gatctgccgc gccgaggtgg gcgacaataa tcccacgcgc aaatcgtccc
     1201 gagctttcga atccaggcgc tcgatcaaag ccagacgacg gcacacccag ctcttgtgcc
     1261 ggcccaggag ctccgccacc tctacctggc tcatgttgtc ttcgcgcacc aaagcgtgaa
     1321 tgatccaagc ttcctccagt tcgcgcgtgc ggccgcccgc gcggttgagc ccgtatatgg
     1381 ccgctttcgc cgagcgctcg tcagcctcca tcaaccgagc tgaaagagat tcgatcccta
     1441 ccagatggcg cgccgcgccc agccgcttga aaccatcgat cagttcgtaa cgttcctggc
     1501 gccggcacac caccacgggc gataactgtc cataccgttt cagcgagcgc gccatggcgc
     1561 gttctgcgtc cggcaagtgc aaacggtagc ggccgtaatg ttcgccgatt tcctggtgtt
     1621 ggagatggag cagatcctgg ctcgcccagc gcagcatgcc tactttcctt cctggacagg
     1681 atgaatgtag gtcagtcatt ttaaggaatc aatatgtcgg gattatctgt tgctccggca
     1741 gtggcgctgc tagccaccac cggcgcgcgc ccgccgccag cgtcgctctc gctcccaaac
     1801 gcgttccatc gcgcgccggc acgcgcggga acagaagcgc tggagcggtg atcgcggccg
     1861 gtgcacgaat ccctcatagc agccggggcg gtcgcagcag tgatcgaaaa aaatatttcg
     1921 tgattaccct cgcggcttct acaactgctt cctgtggcgc gggttgttcc tgttttctga
     1981 cgcgctcccg gtaacgccgg cattgcccgt tgcgcttttc tttgcctgcc gctgtcgccc
     2041 ggtaactctg ctgggccttc caccgcgacc attgccgcgc tgcttgtcgg cactctgggc
     2101 tgcaatagcg ctggcgcgcc tcccgaggac aaaagcgctg ctcacagcct ttcagcagac
     2161 aggtacgggt ccgcggacgg cgagtcggac gtgtccgccg gccatgtccc tcatcttcag
     2221 gcgggcgtgt agaatcctcg atagggccca cgggagtgcg gctcccggaa agctcatgcc
     2281 tcggagagga cttgcattcc tctccggggc gcagcaggtc cggttcactg cgaacttacg
     2341 tactctatcg cagacgaacc cggcgccgcc tggtcccttc tatgattttg tttttcccgc
     2401 ctgcagcggc gcggttggtg agttcgacag ctaggcgtcc ccgcctgtgg gttcctgagg
     2461 ttcgccacat cggtgatccg atctcagcac ccggttatca gctcacaacc tggtttgaaa
     2521 tcagttctgg ttatcgtgtg cgccactggc gccatcatct attggcggcc tggcgctgct
     2581 tggtccgcca tagtggcgcc ttctcaggca gccactaaca tcctttggta gaacgctgaa
     2641 aaacatccat tccttgcgct cggtcaaggc ttgcagcacg aatgtattct gccacataac
     2701 cgacgcaaag gcctcgccga gagactgcag ataacctcac aaacgcgcca ctccaggtct
     2761 tgtcgattgt cggcatcccc cagacggctt gtgtcactcc agttgccact ctatacgact
     2821 caatccctgt taccgtgtca tcaaacgtga tgcatcgtgc gaccgagatg ttcggtgtgc
     2881 cggaaacatt agatgctgtt gaaaaactcc ccttaccgtg tgcgcggaac tttgcctagg
     2941 aaaatgacgc gattagaaac gctataagcg ttcactggcc gcgaggtaga cattctccat
     3001 ccccctctgg aacggtcgga ccgctaaaga agagtttttc aacagcatca ttaccttttg
     3061 gcgccccagc taatctttgg agctgtcgtc tggcgaatgc agtagatgag gcttaacgaa
     3121 acccctctca aatgcttttc ggcatgcaca gcggtttcgg atggttagtg ctttcgatac
     3181 gcgaattgga acctggcgtc ttgcccgtgg tcaaggctgt tgttccaaat ctgcgctact
     3241 agcttttggc ataaccagga ccggcacgcg agccggcgga ggtgggatat ggtcagtttg
     3301 gcctgcgaac tctttcaagg cccgcacgac cgttcccgat ccaatcccta gctggcgcgc
     3361 gatcgctctg atcctcatgc ctcgctggcg cagcctcaac gcctcctcgc gatcgaatat
     3421 tttcttggga cgacccactg gccgtccaga tttcgttccg tgttcgcggg cgtactctag
     3481 acccgccatt actcgttccc ggatcacgga tctttccagc tcggccatag cagcaatgat
     3541 ggtgaacatc gcttttccca tcggtgtgct ggtgtcgagc gcctcctggt ggctgatgaa
     3601 ctcaatgcct agcgtgcgaa actcttccag ggccatcact aactgcttca cgctgcgggc
     3661 gaatcgatca aaccgccaga ccacagccac cccaaacatg ccgcgtcgcg cgtcgtccat
     3721 cagggcctgt agaccgggcc gtgattcctt agcgccgctc atccgatctg agtaaacgcg
     3781 atggagttcc cagccgcgac tggccaacaa gcctacaagc ggctctacct gtgcttcggg
     3841 attctggaga tacgcggaga cgtcaccaaa cttcttcttg cttgtcgttg agcatcgaac
     3901 gtaaattgct cctcgcatgt aacacccaat tgcctcgtgc ggatgactct gatcgagcca
     3961 gcgcaggatg gtccgtggtg ttccaaaagt tgagacaaac ttcggcaagc cgattttgtt
     4021 tttggaacag aatggaacag agttgaggtt ttgggtagct gtaagtactt gaaaatacgg
     4081 tggccagata ggatgagaag gccggtatcc tcggcgggat gcgatatgct cccaatgaga
     4141 aacgcactcg aggtgcaatc gaaaggag
//
LOCUS       DASZAK010000226         2646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_254599, whole genome shotgun sequence.
ACCESSION   DASZAK010000226 DASZAK010000000
VERSION     DASZAK010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254599"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VGT44_03810"
     CDS             <1..217
                     /locus_tag="VGT44_03810"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2579957.1"
                     /translation="EDTAGAGLPGRCDLVAMATHGRTGFQHWVLGSVTERVLGATRLP
                     ILIVRPAETEFHRTGNGSAIFASVEMV"
     gene            complement(223..2202)
                     /locus_tag="VGT44_03815"
     CDS             complement(223..2202)
                     /locus_tag="VGT44_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV2579958.1"
                     /translation="MSDARETRHGSASSPPPIAISSTNVQPFLDISPDGVVIINLAGS
                     IVLVNGQTRALFGYAREELIGQPLGLLLPQRYQASHLAHLTRYFASPRARPMGLGLHL
                     VGRRKDGSEFPVEISLNPLLLDGALHAIGSIRDVSAQRAAEQQRLQQAQQLRLQADLI
                     DLAHDAILVRDPVGRVLSWNKGAEELYGWSAQEALGRVSHILLKTRSPVGRAAIEAQI
                     ERDGQWQGELAHTCRDGHPVTVESRQVLARDEAGQPSAILEIDRDITRRRQQEQSERA
                     VYVETAARLSFLQQVLDAMPSGVYLVSGPEARLLLANRATAGVWGAEWPVDQPLRAFL
                     ATNGIKLFDAQGQPLPPEQCAALRAVRQGETVLQQQETIRRANDSSLPVLVSALPLTW
                     QPGTGQLAPAGETVALVVHQDVSSLKEAEYLKDEFVGIVAHELRTPLAVLRGFADMLL
                     VQTARQRGPELADWQQEALGEIELAVTRLVDLTEELLDVTRLQAGRLQLQRTPTNVVA
                     LARRVAILLQQATTRHQVEVRTEDAHLVADIDPGRIEQVLTNLIGNAIKYSPQGGPVI
                     ITLWEEGAAGIVKISVADRGIGIPAGQQAQIFGRFVRADNAAAWGISGTGLGLYICRE
                     LVERHGGQLWFESQEGAGSTFFLTLPAGAGQGSAI"
BASE COUNT          460 a          833 c          841 g          512 t
ORIGIN      
        1 ggaagatacc gcgggcgcag gcctccccgg tcgctgcgac ctggtcgcga tggcgacgca
       61 tggccgcacg ggcttccagc actgggtgct gggcagcgtc accgagcgcg tactgggcgc
      121 aactcgtctg cctatcctga tcgtgcgtcc tgctgaaacg gagtttcacc ggacaggcaa
      181 tggcagcgcc atctttgcgt cggtagagat ggtgtagcaa gactatatgg cgctcccctg
      241 cccggcgccg gcggggagcg tcaagaagaa ggtcgaacct gcgccttcct gcgactcgaa
      301 ccagagctgc cccccgtgcc gctccaccaa ctcgcgacag atgtaaagtc ccagcccggt
      361 gccgctgatg ccccatgctg cggcattgtc ggcccgcacg aagcgcccga agatctgcgc
      421 ctgttgcccg gcgggaatgc cgatgccgcg atcggccacg ctgatcttca ctatccctgc
      481 cgcgccttct tcccacagtg tgatgatcac cgggcctccc tgcgggctgt acttaatagc
      541 gttgccgatc aggttggtca acacctgctc gatgcgacca gggtcgatat cggccacgag
      601 gtgcgcgtct tcggtgcgca cctcgacctg gtgccgcgtc gttgcctgtt gcaggagtat
      661 tgcgacgcgc ctggcgagcg cgacgacatt ggttggagtg cgctgcagct gcaagcgtcc
      721 ggcctgcaag cgcgtcacat ccagcagttc ctccgtcagg tctaccaggc gcgtgaccgc
      781 cagctcgatc tccccaagcg cttcttgctg ccagtcggcc agctcggggc cgcgctggcg
      841 cgccgtctgc accagcagca tgtcggcaaa gccccgcagc accgccagcg gcgtgcgcag
      901 ttcgtgcgcc acgatgccca cgaattcgtc cttgaggtac tcagcctctt tgagggagga
      961 aacatcttga tggaccacca gcgccaccgt ttctccggcg ggtgccagct gacccgttcc
     1021 cggctgccag gtcaagggca gcgcgctgac cagcacgggc aggctggagt cgttggcgcg
     1081 gcggatggtc tcctgctgct gtagcaccgt ctctccctgt ctgacagccc gcagtgcagc
     1141 gcactgctca ggtggcaagg gttgtccctg cgcgtcgaag agcttgatcc cgttggtcgc
     1201 caggaacgcg cgcagcggct gatcgaccgg ccactctgct ccccatacac cggcggtcgc
     1261 gcggttggcc agcagcaggc gcgcttctgg tcccgagacg agatataccc cgctcggcat
     1321 ggcatccagc acctgctgca agaagctcag acgcgcagca gtttcgacgt acacggcgcg
     1381 ctcggactgc tcctgctggc ggcgccgggt gatatcgcgg tcgatctcca ggatggcgct
     1441 gggctgtccc gcttcatcgc gcgccagcac ctggcggctt tccacggtca ccgggtggcc
     1501 gtcgcggcag gtatgggcca gttcgccctg ccactgaccg tcgcgctcga tctgcgcctc
     1561 gatagcagcg cggccaacag gggagcgcgt tttgagcaag atgtggctta cgcggcccag
     1621 ggcttcctgc gcgctccaac catagagttc ctcggccccc ttgttccagg agagtacgcg
     1681 gcccaccggg tcgcgcacca gaatggcgtc gtgcgcgagg tcgatcaggt cggcctgcag
     1741 gcggagttgc tgggcttgct ggaggcgctg ctgctccgct gcgcgctgcg cgctcacatc
     1801 gcgaatggag ccgatggcgt ggagcgcgcc atcgagcagc agggggttca ggctgatctc
     1861 caccgggaat tctgaaccgt ccttgcgcct cccgaccagg tgcaacccga gacccatcgg
     1921 acgggcacgc ggggaagcga agtagcgcgt cagatgcgcc aggtgggacg cttgatagcg
     1981 ctgggggagc agcaggccga gcggctgacc gatcagttct tcgcgggcat agccaaagag
     2041 cgcccgggtc tgcccgttga ccagcacgat gctcccggcc agattgataa tcaccacccc
     2101 atccggcgag atgtcgagaa acggctggac gttggtggaa gagatagcga tgggcggagg
     2161 tgatgaggcg gaaccgtgtc gggtctctcg tgcgtcggac atcgatgtat tcctttgctc
     2221 acttggcgca tgaaatatct taatttcatg tgttgattat aaaccaggga tgcttgccca
     2281 tcaagggagc aatgatacgc tacttgtgat gtgttgaccg gcttcggcgc cttcttcggg
     2341 ctgttcatga acatgaactg cctgctggcg ggcgcggtga gtatcaactc ccttatcggc
     2401 gggctggccc tgttctgaca ccatcccgag tgtcgtgtaa aaggagagag attttaccga
     2461 gtaaagggat tggcatgatc aaaccaggac acacagtatg atcccagaat ctccgtatgc
     2521 caaattcaat ttctgcgtgt ttcccacgat gctttgccaa cgccttccgg ctataataag
     2581 agtgtcagcc ggggaaccat ttccgtggct ttcggtttcc ttgatcaaga aaagagatag
     2641 gaagtt
//
LOCUS       DASZAK010000227         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_325544, whole genome shotgun sequence.
ACCESSION   DASZAK010000227 DASZAK010000000
VERSION     DASZAK010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325544"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..514
                     /locus_tag="VGT44_03820"
     CDS             236..514
                     /locus_tag="VGT44_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEV2579959.1"
                     /translation="MSQITLHLPGCHSLKEKRQVIKSVMARVRQQFEVAIAEVDEQDR
                     WQIAKLGVSCVSNSSQHSDAILGHVLRYIEETRPDIQVTNSEKEIINW"
     gene            566..1354
                     /locus_tag="VGT44_03825"
     CDS             566..1354
                     /locus_tag="VGT44_03825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV2579960.1"
                     /translation="MSSHLESMLLQTLPAYVLGVRVDRLSQQQALDRIEQMIALHRSG
                     GNALSCQQIITVNTEFVMVAQHDTAFRHTINSAALAIADGIGVVWATHFVHAPAPERI
                     TGTDTLVELAKRCAVKGYRLYLLGAAPGVAQQAGARLQALAPGLELAGTYAGSPAPGE
                     EDAIIERIRAAHADVLCVAYGAPAQDLWIRRNLSRLPVAVAMGVGGAYDFLSGRQRRA
                     PYIMQRMGLEWLYRLYREPWRWRRMLAIPRFMLQVILRGRKKYD"
     gene            1347..2366
                     /locus_tag="VGT44_03830"
     CDS             1347..2366
                     /locus_tag="VGT44_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HEV2579961.1"
                     /translation="MTDPLHIARSRLHSLIAGFAGKRVLVIGDMVADEYLIGRPTSIS
                     REAPVLILKLSEERTIPGGAANVAVNARTLGAEVFLAGVVGDDLPGQRLRRAISDLQM
                     HQEGLVTDTQRPTSTKTRILAGSSQIVQQHIVRIDRVDTSEVGQPGKGCIIDYIQRVL
                     PTIDAVVLSDYENGVISPEIIETCLPAARQLDKVIVVDSHGSLFRFQGVTALTPNQPE
                     AELTLGVTINSQAELEQAGKRLLDGSHAQSVLITRGGEGMSLFEVGKSPLHLPIHHLD
                     HSIEIVDTNGAGDTVAATFTLALTAGASFLEAAYLANAAAALVVRRLGCASNTPAELL
                     GAITW"
BASE COUNT          546 a          661 c          697 g          542 t
ORIGIN      
        1 gcagtcagtt tatgtgccat taccgggtgc gaagaaaaat ggacacgggg aagattcagc
       61 acctcatctc gcctgggctg tggcacgcct tgcatgggca aacttggatg ctcgccgctc
      121 gaagtagttg tttcagtcat gggaacaagc tcctttgtta ttatgtttcc ataacatttt
      181 accagagttg gctagagggc gtcgactctg ttatactagc tgcatgatag taggagtgag
      241 ccagattacg ctgcacctgc cgggatgcca ctcattgaaa gagaaacgcc aggtaataaa
      301 atctgtcatg gcacgtgtgc gccagcagtt tgaggtagcg attgcagagg tcgatgaaca
      361 ggaccgctgg caaatcgcga aacttggcgt gagttgcgtt agcaatagta gccagcatag
      421 cgacgcaatc ctgggccatg tgctgcgcta tatcgaagaa acgcgcccgg acattcaggt
      481 taccaactcc gaaaaggaga tcatcaactg gtagaaaatc tcgggtcaac tgcaaagtca
      541 actggcccaa gatgaggaag caagtatgtc gtcacattta gaaagtatgc tgttacaaac
      601 tctacccgcc tatgtccttg gtgtgcgcgt agaccggctt tcacagcagc aggcgctcga
      661 taggatcgag cagatgatcg ccctgcatcg ttccggcggt aatgcgttat cctgccagca
      721 gatcatcacc gtcaataccg aatttgtaat ggtggctcaa cacgatacag catttcgtca
      781 taccatcaat tcagccgcgc tcgccatcgc cgatggcatt ggagtcgtct gggccacaca
      841 ttttgtgcac gcgcccgcgc ctgaacgcat tacaggcact gatacactgg tagaattggc
      901 caaacgttgt gcagtaaagg gctatcgtct ctacttgctc ggagcggcgc caggggtggc
      961 gcaacaagca ggtgcgcgct tacaggcgct cgctcctggg ttggagctgg ctggaaccta
     1021 tgctgggtca cctgcccctg gcgaagaaga tgcaattatt gagcgtattc gagccgcaca
     1081 tgctgatgta ctgtgcgtag cctatggcgc acccgcccag gatctctgga ttcgccgcaa
     1141 tctctcgcgc ctgcccgtag ccgtagcgat gggcgtagga ggggcatacg attttctatc
     1201 gggccgtcag cgccgtgcac cctatatcat gcagcgtatg gggctggaat ggctctatcg
     1261 cctctaccgt gagccctggc gctggcggcg catgctggcg atcccccgct tcatgctcca
     1321 agtaatactc agagggcgta aaaaatatga ctgatcctct tcatatagcc agatcgcgac
     1381 tgcactcact gattgccggc ttcgcgggta agcgcgtatt ggtgataggg gacatggttg
     1441 ccgacgagta cctcatcggt agaccgacaa gtatttctcg tgaagcgccg gttttgatcc
     1501 tgaaactgag cgaggagcgc actattcctg gcggcgcagc taatgtggca gtcaatgccc
     1561 gcacactggg cgcagaagtt tttctggcgg gtgtggtcgg cgacgatttg cctggccagc
     1621 gtctacgccg cgcgatcagt gatctgcaga tgcaccagga gggcctggtg accgatacac
     1681 agcgtcctac atcaacgaag acgcgcattc tcgctggtag ctctcagatt gtgcagcagc
     1741 acatcgtgcg catcgaccgc gtggatacct cggaagtagg ccagccgggc aaaggatgta
     1801 tcatcgacta cattcagcgc gtgttgccaa cgatcgacgc ggttgtactc tcggattatg
     1861 agaatggtgt cattagcccc gaaattattg aaacctgtct tcccgctgcg cggcaattgg
     1921 ataaagtaat tgtagtggat tcgcatggct cgctcttccg ctttcagggc gtcaccgcgc
     1981 tcactcccaa ccagccggaa gcagagctca ccctgggagt gaccattaac tcgcaggctg
     2041 agcttgagca agcagggaag cgcctgctgg atggcagcca tgcacaaagt gtcctgatta
     2101 cgcggggtgg ggaaggcatg agcctgtttg aggtaggcaa atcaccgctg cacctgccca
     2161 tacatcacct ggatcatagc atcgaaatcg ttgataccaa cggtgcgggt gatactgtgg
     2221 cggcaacctt tacgctggct ctcactgccg gcgcgagctt cctggaagct gcctacctgg
     2281 cgaacgccgc cgcggcgctg gttgtacgcc gccttggatg tgccagcaac acgccagcgg
     2341 aattgctggg cgcaatcaca tggtaaatgc cctacgaagt gagcaggttt tgccctccaa
     2401 catgagcatt tgccctcaca tgtgagcact gggacacacg ttccct
//
LOCUS       DASZAK010000228         3577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_645395, whole genome shotgun sequence.
ACCESSION   DASZAK010000228 DASZAK010000000
VERSION     DASZAK010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645395"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VGT44_03835"
     CDS             <1..844
                     /locus_tag="VGT44_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2579962.1"
                     /translation="HDITERKSRDDKDDTTNNPLQEAAQPLKEAAQDLIVQARKEVET
                     TRLPWYRVVRGARILLIVDIVLLALFSLLTWWVHLHPVLSLDVTITREFQENQSAWLK
                     YFMMAVSFQGYNLWFNVSLIAIAAVVFWVVDLRLEAIVLVLLTCVSAVLNVVIKILVD
                     RPRPSARLVDIIQGAGGPSFPSGHVMSYVAFWGLLFSFGIILFSGTRWWRITLMIVSG
                     LIVALVGPSRIYLGDHWASDVLGAYLIGFALLIVTLWIFLLLKQRGVLAPRRQHSWYR
                     HTRS"
     gene            complement(857..1498)
                     /locus_tag="VGT44_03840"
     CDS             complement(857..1498)
                     /locus_tag="VGT44_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579963.1"
                     /translation="MAKKQSTQDPTAGSSEQNEKQEKKLAKREAKAMLAVDEAKKRLA
                     KADRKLADAQLRVEKRRTSLQAADAHLAELRNSHQPAGATDAASGEASDAQDTTDATE
                     MANAASEDSEAPVLVAHEATNADESSVSANGASNPAADTQEEAPAQATEEPSAVASEA
                     APEGASEAAEPAEESASQSEETQGATEEGESTKAEETPWWHREPYNPYGQQEG"
     gene            1754..2992
                     /locus_tag="VGT44_03845"
     CDS             1754..2992
                     /locus_tag="VGT44_03845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2579964.1"
                     /translation="MLEPHESIIAGRYRLLGRLRRGGMSEVFLSLDEQTQQHVALKLV
                     SNDTPDCYDRLKHEIRVLSMLSHKHIIPIMDHGEWENYAYLVMSHMKHGNLRSLLDQG
                     PLSRERAGKILSQVASALQYAHEQGILHRDIKASNILLKSIEEDDIYLADFGLAKALG
                     EGSDITQTGYLIGTPEYMAPELVTMAESVSSEIYALGILLYKMLTGRMPFTGTTPLAI
                     CWKHVYEVPPLPSSLNPAIDPALEAIILRAIDKDPARRFPTVQNMAEAYEQALLLCRP
                     SPVLSRPMAPAYVEASRLGQYAITSGAFPRERSFTRLSAFKRNSLLALVALLWLLTSF
                     GLGFMVAHSGVQNIVASSIHAHSVGQAVHPVKVDPPVKLIPPVRQVSSPPPTSATTHS
                     PPPITLRKHHKHDDGGGGDD"
     gene            3079..>3577
                     /locus_tag="VGT44_03850"
     CDS             3079..>3577
                     /locus_tag="VGT44_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV2579965.1"
                     /translation="MQNLEPHNLIEASPLFRDLQPQETASILARLQPVIVPGGTHILT
                     RGAWHGPLYIIASGQVSVLLREEREEREQLNAETDGLAVARLGPGECFGEMSLLTGEP
                     PSATIRAEQETLLWTLPQADFLALLGECPTLTRNINAILSLRLARANRQILLNHTAER
                     VWLADF"
BASE COUNT          734 a         1046 c          939 g          858 t
ORIGIN      
        1 acacgatatt accgagcgca agtctcgaga cgataaagac gataccacca ataaccctct
       61 gcaggaggcc gcccaaccgc taaaggaggc tgcgcaagat ttgatcgtcc aggcgcgcaa
      121 agaagttgaa acgacgcgcc taccctggta ccgggttgta cgtggtgcgc gtatcttgct
      181 gatcgtggat atcgtattgc tggcgctttt cagcctgctg acatggtggg tgcatcttca
      241 ccccgtgctg tcactcgacg tgacgataac gcgcgaattt caggaaaatc agtctgcctg
      301 gctgaaatac ttcatgatgg ccgttagctt tcaaggctat aatttgtggt tcaatgtcag
      361 tttaatcgcc atcgccgccg tggtattctg ggtggtcgat ttacgcctgg aagctatcgt
      421 tctcgtgctg ctcacctgtg tcagcgcggt actcaacgtg gttatcaaga tactggttga
      481 ccgcccacgt cccagcgctc gcctggttga catcattcag ggagctggag ggccaagttt
      541 cccaagcggg catgtcatgt cttacgtggc cttctggggc ttgctcttct cattcggcat
      601 tattctattt agcggcacgc gctggtggcg cattactttg atgatcgtat ccgggctgat
      661 agttgccctc gtcggcccct cgcgcatcta cctcggcgac cactgggcca gcgacgtgct
      721 gggcgcgtac ttgatcgggt tcgcgctgct gattgtcaca ctctggatct tcctgctcct
      781 caagcagcgc ggcgtgctgg cgccacgcag gcagcattca tggtaccggc acacgcgcag
      841 ttgatcggct ggtcagctat ccttcttgct gaccataggg gttgtaaggc tctcgatgcc
      901 accagggtgt ctcctcagcc ttggtcgact cgccttcttc tgtagcgccc tgagtctctt
      961 ctgactgaga agccgattcc tccgcgggtt ctgctgcttc actcgcccct tcaggcgcgg
     1021 cttcgcttgc caccgcgctc ggctcttctg tcgcctgcgc cggggcctct tcctgggtat
     1081 ctgctgcggg attcgatgcg ccattcgcgg acacggacga ctcatctgcg tttgtagctt
     1141 catgcgctac aagtacaggt gcctcagaat cttcggaagc cgcattcgcc atttctgtag
     1201 cgtccgttgt gtcttgagcg tcgctggctt ccccagatgc ggcatcagtt gcgcccgcgg
     1261 gttgatgcga attgcgcaat tcggccaggt gcgcatccgc cgcttgcaag ctagttctgc
     1321 gcttctccac gcgcaattga gcatctgcta gcttcctatc ggccttcgcg agcctctttt
     1381 tagcctcatc gaccgccagc atcgccttcg cctcccgttt tgccagtttc ttctcctgct
     1441 tctcgttctg ctcgctcgat ccagcagtag ggtcctgtgt gctttgcttc tttgccatga
     1501 caatctcctc ttttgcgcaa aatggggttc agatagggtg tcaaccgctt cactttgata
     1561 ctatcatact cttcatcgaa atgcgagtgt tttgatcaaa aatggataat actgcacttt
     1621 accaatttgc tccgctattc tttcagcgcc cgggtcggtc aataaggctg agtcacgaat
     1681 cgctctttgt aaacgcatta catagagatg gtacaacaaa tagtaccgca aacgaaaaag
     1741 acaggacctc cccatgctag aaccgcacga atccatcatc gccgggcgtt atcgcctttt
     1801 gggccgtctg aggcgcggtg gaatgtcaga agtttttctt tcgctcgacg agcagacaca
     1861 gcaacacgtt gcgctgaagc tggtcagcaa tgataccccc gattgctatg atcgtctcaa
     1921 acacgagatt cgtgtactga gtatgctttc acacaagcat atcataccaa tcatggacca
     1981 tggtgaatgg gagaattatg cctaccttgt tatgtctcac atgaagcacg gcaatctgcg
     2041 cagtcttctt gatcaaggcc ccttaagccg ggagagagcg ggcaaaatcc tgtcgcaggt
     2101 ggcaagcgcg ttgcaatacg cgcatgaaca aggcatcctc catcgagata tcaaagcatc
     2161 caacatattg ctcaaaagca tagaggaaga tgatatctat cttgccgact ttggcctggc
     2221 gaaagcgctg ggagagggca gcgacattac tcaaaccggc tatctgatcg gcacgccgga
     2281 gtatatggct cctgagctgg tgacaatggc tgaaagcgtc agcagcgaga tttatgccct
     2341 tggcattctg ctatacaaaa tgcttacggg taggatgccc tttaccggta ccacccctct
     2401 tgccatttgt tggaaacatg tttacgaagt acccccgctt ccttcttcgc tcaatcctgc
     2461 gattgacccg gcgctagaag cgatcatcct gcgtgctatc gacaaagacc ctgctcgtcg
     2521 tttcccaact gtgcagaaca tggccgaagc gtatgagcag gcgctgctcc tttgccgacc
     2581 ctcgcccgtc ctctcccgac caatggcccc agcatatgtt gaagcatcca ggctgggaca
     2641 gtatgcgata acatcaggtg ccttcccgag ggaaaggagc tttactcgcc tgagcgcttt
     2701 caaacgcaat agcctgttgg cgctcgtcgc gcttctatgg ctgcttacct cctttgggct
     2761 aggatttatg gtcgcacatt cgggcgttca gaacatcgtc gcctcaagta ttcatgccca
     2821 cagcgtagga caggccgtcc atcctgtgaa agttgaccca cctgtgaaac taataccacc
     2881 agtgcgacag gtatcatctc cgccgccaac ttcggccaca acacattcac ctccaccgat
     2941 aacactccga aaacatcaca agcatgatga tggaggaggt ggcgacgatt aacggtagat
     3001 gaggcaataa tcggcaaact gttccatacg ttttcgtggc tgtccaggat ggctgagcca
     3061 tgatacaata atgggcgtat gcagaacctg gagccacaca acctgattga ggccagcccg
     3121 ctgtttcgtg acttgcagcc gcaggaaaca gcaagtatcc tcgcgcgctt gcagccggtg
     3181 atcgtacccg gcggaacaca tatcttaaca cgcggtgcct ggcatgggcc gctctatatt
     3241 atcgcgagcg gccaggtgag cgtcttgctg cgcgaggaac gcgaggagcg cgaacaactg
     3301 aacgccgaga ccgatgggct ggccgtggcg cgcctgggac caggcgaatg cttcggtgaa
     3361 atgtccctgc tcaccgggga gccgccctcg gcaacgatac gcgcagagca ggagacgctg
     3421 ctctggacgc tgccccaggc cgatttcctg gcgctgctcg gcgaatgtcc cacgctgacg
     3481 cgcaacatca acgccattct atccctgcgg ctggcgcgcg ccaaccgcca aatcctgctg
     3541 aaccacaccg ccgagcgtgt ctggctggcc gacttcg
//
LOCUS       DASZAK010000229         4871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_396375, whole genome shotgun sequence.
ACCESSION   DASZAK010000229 DASZAK010000000
VERSION     DASZAK010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396375"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2039)
                     /locus_tag="VGT44_03855"
     CDS             complement(<1..2039)
                     /locus_tag="VGT44_03855"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEV2579966.1"
                     /translation="MTTYTIEVRAIAERYDILAQQVARELLLLPTLASGQASYSIRTA
                     RLYHLTGSLSPTAIDQLTRHLFIDPVTQQATISTTSTPTDTSSPPTVPQQNASSNDNS
                     TVVDVFFHPGVTDTLAESIQAGATLLGIHGLERVETGQRYFLDARLNEDEVRLITDAL
                     LYNPVIQEYSLRMASIELDTPSSLDHPNTRRGRFIAPTADLSAPTNTITNAASGEADK
                     SAVGAINRPLQGDHHHVGMIQLTTMRDEQLVEMSRQGLLALNLDEMRTIQRYYREQGR
                     EPSDVEMETLAQTWSEHCSHKTFKATIEYREEDGEGNVIERETIAGLLKQYIMRATQQ
                     VARPWVVSAFSDNAGIVRFTETQDIAFKVETHNHPSAIEPFGGANTGVGGVIRDVLGV
                     SAQPIACTDILCFGPPDTPAMALPPGVLSPHRIAGGVVNGVRDYGNKMGIPTVNGAIL
                     YHPGYIYNPLVFCGCLGILPHGSHPRAAQPGDAIVALGGRTGRDGIHGATFSSGEMSR
                     EITAQAGSAVQIGAPITEKKVADVIIQARDRRLYHAITDCGAGGFSSAIGEMAAETGA
                     YVELEKAPLKYQGLAPWEIWLSEAQERMVLAVPPQHLDELLALCAIEEVEASVLGSFT
                     DTQRLVVTYAGQTVADLDMTFLHDGRPERRLEAVWTAVGAGADDGRGGDACVARVP"
     gene            complement(2133..>4871)
                     /locus_tag="VGT44_03860"
     CDS             complement(2133..>4871)
                     /locus_tag="VGT44_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2579967.1"
                     /translation="NHPLQVLQVLDYAIQLCDVLTHLHQRYEPIIFRDLKPSNIMIRP
                     DGYVFLIDFGIARNFKEGQKDDTEIFRTPGYAAPEVVFAQTDARSDIFSLGVTMHQCL
                     TRQALDYFDHNQAFPPARQFNAEVSEELSDLIACMVQFMPGDRPTSAAEIGRRFERIY
                     QQVLSTQARFGQSGQQEYRPLSFYSDDTEAGQPAFWRNPVRLRAVTPLVRLPTLFMAG
                     LMWLLMPLIVGMRQATQRVVSTGMRQAVYDKYLYVRVRLNAASLWTDRFLLLLFSLLA
                     GSVALSVAIYTRPGSSYARVEFGLAAALLVTTIISGGLLRNAVARHIMMYTGILVAIA
                     FLALLASPNYQAGSTPGTHQSMTLNLLLTYGTVIVMCIALVGGIASMRARGSAARAPT
                     RADSLAASEFVRLGHFAVAAIAGLCLLLQFAFGSQEQVPFAPFAPRPFAFVFSQSPLV
                     TSNVIPLIALGLIALYELKRYAQPFAARDRVMICALSIIFMFAQYTFGLNELSHAFPG
                     ASTITLASLNLVLFGAPLVLALLSLPFLPDNLAWVGLVPAFALSLSVAWLQGYLGNQE
                     SISLFAPIAPGTSGGQQGIAAFVQLTSFGQLLFACLGAAGALLLFRILFARKRPALMP
                     GVADRIALLIVALGSGLIQWSFWQGILQHTLAFDYALQGTTLLYTALASFATGLVMVV
                     AALVSTIIASINALSQSSQAHPRAAKVALTLARLATLCCAIVTLLLLNFFGSRGGWLA
                     RLNYLSTISPSLPGFFFSNSEALLTALVLFAFFTLVFLFRPNRAFGRVERITLLLSGI
                     GALLLLTDTGDIQLLPFFSLDMQQIAGSVAASLSIDRVVTACILLAALLSFFWLTRTR
                     LLGDRLVLLFISGASFILALVGAVSPHPLLMILALLAMTQGVLIAAKIEKVRRGNV"
BASE COUNT         1047 a         1409 c         1528 g          887 t
ORIGIN      
        1 gggacgcggg cgacgcaagc gtccccgccc cgcccatcgt ccgcccccgc ccctacggct
       61 gtccataccg cttccagcct ccgttccggt ctgccgtcgt gcaggaaggt catgtcgagg
      121 tcggcgacgg tttgcccggc ataggtgacg acgaggcgct gggtatcggt gaaggagccg
      181 aggacgctgg cctcgacttc ttcgatggcg cacagcgcca gtagttcgtc gagatgctgc
      241 ggcggcacgg ccagcaccat gcgctcctgc gcctctgaaa gccagatttc ccagggggcc
      301 agcccctggt atttaagcgg cgctttttcc agttcgacgt atgcgccggt ctcggcagcc
      361 atttcgccga tggccgagga gaagccgccg gccccacagt cggtgatagc atgataaagc
      421 cgcctatccc gcgcctggat gatgacatcc gcgacttttt tctcggtgat gggtgcgccg
      481 atctgtaccg cgctcccggc ctgtgctgtg atctcgcggc tcatttcgcc agacgagaag
      541 gtcgcgccgt gaatgccgtc gcgccccgtg cgaccaccca gcgcgacgat ggcatcgccc
      601 ggctgtgctg ctcgcgggtg gctgccatgc ggcaagattc ccaggcagcc gcagaagacg
      661 agcgggttgt agatatagcc ggggtgataa aggatcgcgc cgttgacggt gggtatgccc
      721 atcttgttgc cgtagtcgcg cacaccgttg accacgccgc cagcgatgcg atgtggcgac
      781 agcacaccgg ggggcagcgc catcgcgggc gtatccggcg ggccaaagca gaggatatcg
      841 gtacaggcga tgggctgcgc cgaaacgccc agcacgtcgc gaatgacgcc gccgacgccg
      901 gtgttcgcgc ccccaaacgg ctcgatagcc gagggatgat tatgcgtctc caccttgaag
      961 gcgatgtcct gtgtctccgt aaagcgcacg atgcccgcgt tatcgctgaa ggcggacacc
     1021 acccagggcc gcgccacctg ctgcgtcgcc cgcataatat actgcttaag caggcccgcg
     1081 atggtctcgc gttcgatcac gttgccctcg ccgtcctcct cgcgatactc gatggtcgcc
     1141 ttgaaagtct tgtgcgagca atgttccgac caggtttgcg ccagcgtctc catctccaca
     1201 tcgctcggtt cgcgcccctg ctcccgataa tagcgctgga tagtgcgcat ctcgtccagg
     1261 ttcagcgcca gcaagccctg cctgctcatc tccacaagct gctcatcgcg catcgtcgtc
     1321 aactgtatca ttccgacgtg gtgatgatcc ccctgtaggg gccgatttat cgcgcccacc
     1381 gccgatttat cggcctctcc gctcgctgca ttggtgatgg tgttcgtggg ggccgataaa
     1441 tcggcggtgg gcgcgataaa tcggccccta cgtgtgttgg gatgatcgag tgatgagggc
     1501 gtgtccaatt ctatactggc cattcgcaag gagtattcct ggatcaccgg gttgtagagc
     1561 agcgcatcgg tgatgagcct tacctcgtcc tcgttcaggc gcgcgtcaag gaagtagcgc
     1621 tgcccggttt cgactcgttc cagtccgtgt atgcccagca gggttgcgcc cgcttgaatg
     1681 ctttcggcca gggtatcggt cacgccagga tgaaaaaaga catcgacgac tgtgctgtta
     1741 tcgtttgaac tggcgttttg ctggggaacg gttgggggag agcttgtatc ggtgggtgtg
     1801 gatgtggtgg agatggtggc ctgctgggta acggggtcga tgaagaggtg gcgggtaagc
     1861 tggtcaattg ctgtggggga aagcgagcct gtcaggtgat acaatcgtgc ggtacgaatg
     1921 ctataactgg cctggccgct cgccagagta ggcaagagta gtaattctcg agcaacctgc
     1981 tgagccagta tgtcataccg ctccgcgatt gctcgtacct caatggtgta tgtagtcacg
     2041 aatccgttcc tgaaataacc atgcgctgcg atcaatcagt gttgttcacg ggaacagttg
     2101 tattgtagca cgtggacagg aagagtagca aattatacat tcccccgtcg caccttctcg
     2161 attttcgccg cgatcagcac accctgcgtc atcgccagca acgccaggat catcaggagc
     2221 gggtggggcg agacggcacc gaccagcgcc aggatgaatg atgcgccaga gatgaaaaga
     2281 agaaccaggc gatctcccag caggcgtgtg cgtgtgagcc agaagaagga gagcagcgcg
     2341 gcgagcagga tgcacgcggt cacaacgcgg tcgatgctga gcgaggcggc gacgctgccc
     2401 gcgatctgct gcatatcgag ggagaagaac ggcagcagct gaatatcgcc tgtgtcggtc
     2461 aggagcagca gcgcgccgat gccgctgagc agcagcgtga tgcgctctac ccgtccaaag
     2521 gcgcggtttg gccggaacag gaaaacaagg gtgaagaacg caaacagcac aagcgcggtg
     2581 agcagtgcct cgctgttgga aaagaagaag cctggaagtg atggcgaaat ggtcgagagg
     2641 tagttcaggc gcgccagcca gccgccgcga ctgccaaaaa agttgagcag cagcagggta
     2701 acgatggcgc agcaaagcgt cgccagccgg gcaagcgtaa gcgccacctt tgccgcgcgc
     2761 ggatgtgcct gcgaggactg agaaagcgcg ttgatggagg cgataatggt tgaaacgagc
     2821 gccgcgacga ccatcaccag gccagtagcg aacgaagcaa gcgcggtgta gagcagcgtc
     2881 gttccttgca gcgcgtagtc gaatgccagc gtatgctgta agatgccctg ccagaacgac
     2941 cactggatca gcccactgcc cagcgctaca atcagcagcg cgatacggtc ggcgacgcca
     3001 ggcatgagag ctggtcgctt gcgggcaaac aggattctga aaagcagcag cgccccggca
     3061 gcgccaagac aggcaaagag cagctggccg aacgacgtta attgtacgaa agcggcaatg
     3121 ccctgctggc cgccgctcgt tccaggcgcg ataggggcga acaacgagat cgattcctgg
     3181 ttgcccaggt agccttgcag ccaggcaacg ctcagactca gtgcgaacgc tggcacgagt
     3241 cccacccagg cgagattgtc cggcaaaaaa ggcagcgaga gcagagccag cacgagcggc
     3301 gcgccgaaca gcaccaggtt gagcgatgcc agggtaatcg tcgatgcgcc gggaaaggca
     3361 tggcttagtt catttagccc gaaagtgtac tgcgcgaaca tgaaaatgat gctgagcgcg
     3421 catatcatca cacgatcgcg agcggcgaac ggctgcgcgt agcgcttgag ttcgtagagc
     3481 gcgataagtc ccagcgcgat gagtggaatg acgttggatg taacgagggg agattgtgaa
     3541 aagacgaagg cgaacgggcg cggcgcgaag ggcgcaaagg ggacttgctc ctggctccca
     3601 aaggcaaatt gcagcagcag gcagagtccc gcgatagcgg ctacggcgaa gtggcccagg
     3661 cgaacaaact cgctcgctgc cagcgaatcg gcgcgggtcg gcgctcgagc ggcgcttccc
     3721 cgcgctcgca tactcgcgat accgccaacc agcgcaatgc acatgacaat caccgttcca
     3781 taggtgagca gcaaattcag cgtcatggat tgatgcgtgc caggtgtgct gcccgcctgg
     3841 taattaggtg aggcgagcaa cgcgagaaag gcgatggcca ccaggatgcc tgtatacatc
     3901 atgatatgac gcgccactgc attgcgcagc agaccgccgc taataatcgt cgtgacgagc
     3961 agcgccgctg ccaggccgaa ctcgacgcga gcataagaac tgccaggccg agtataaatc
     4021 gctacactga gggcaaccga accggccagc aggctgaaca gcagtaacaa aaagcgatcc
     4081 gtccacaggc tggcagcgtt cagccgcaca cgcacataca ggtacttatc gtagaccgcc
     4141 tggcgcatcc ccgttgatac cacgcgctgc gtcgcctggc gcattccgac tatgagcggc
     4201 atgagcagcc acatcaggcc cgccatgaac agcgtgggca atctcaccag cggcgtaacg
     4261 gctcgcaggc gcaccgggtt acgccagaag gcaggttgcc cggcttccgt atcgtctgaa
     4321 taaaatgata gaggacgata ctcttgctgt cctgattgcc caaaccgtgc ctgtgttgag
     4381 agtacctgct ggtagatacg ctcgaaccgt cgcccaatct cggctgcgct cgtaggccga
     4441 tcacccggca tgaactgcac catgcacgca atcaagtcac tcaactcttc ggagacttca
     4501 gcattgaatt gtcgcgcggg gggaaacgcc tggttgtggt caaagtaatc gagcgcctgg
     4561 cgcgtcaggc actggtgcat ggtgacgccc agactgaaga tgtcggaacg cgcatctgtc
     4621 tgcgcgaaga ccacttcagg cgcggcatag ccgggtgtgc gaaagatctc cgtgtcatcc
     4681 ttctgcccct ctttgaaatt gcgcgcaatg ccgaaatcaa tcaaaaacac gtacccatcg
     4741 ggccgtatca taatattcga tggtttcagg tcgcgaaaga tgatcggctc atagcgctgg
     4801 tgcaggtggg tcagcacatc gcacaactga atcgcataat ccagcacctg taatacttgc
     4861 agggggtggt t
//
LOCUS       DASZAK010000230         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_5791, whole genome shotgun sequence.
ACCESSION   DASZAK010000230 DASZAK010000000
VERSION     DASZAK010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5791"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..232)
                     /locus_tag="VGT44_03865"
     CDS             complement(77..232)
                     /locus_tag="VGT44_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579968.1"
                     /translation="MTLVSVDHVPDETYTEVRKQFTDEELVYLTLAIISINGWNRLAI
                     SFRTVPQ"
     gene            complement(245..361)
                     /locus_tag="VGT44_03870"
                     /pseudo
     CDS             complement(245..361)
                     /locus_tag="VGT44_03870"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(362..529)
                     /locus_tag="VGT44_03875"
     CDS             complement(362..529)
                     /locus_tag="VGT44_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579969.1"
                     /translation="MEMQPRLDYGKIVQGVGRAMSGLDQLVRSSNLASQEPLLIELVK
                     IRASQINGCAY"
     gene            837..>2261
                     /locus_tag="VGT44_03880"
     CDS             837..>2261
                     /locus_tag="VGT44_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV2579970.1"
                     /translation="MATTAQELRITKTMLPPGPPRLPYIGNLLSLRQDPLGFLQQLQR
                     TYGNMATIYVGKNPAVLLFRPEHVRYVLVEHPRNFANRGVRQGNSDGGFANEGLLTID
                     GDKHRQQRHAVQPAFHKKRVEGYGAIMEQYTQELLKTWHPGDTVDMSRAMQALTLRIV
                     SKCLFSIDLSSQLGPLSDAFDGVIGSSTSMAEDLLNIRIDNPITGYGKRMAAMRQLDM
                     LIYTLIAQRRDDDRDYQDVLSMLMSAQSGVEPGTKLTEKQVHDHILTFLAAGHETTAI
                     ALVWTFYLLSQHPRVRIKLQDEIRSVLAGREPTLDDLAKLPYLDWVLSESMRLYPPAW
                     LQMRFVTKESTIDGVRLPVGTLLILSQWVIHRLPDIWQDAEVFKPERWDPANEQQIPP
                     GAYFPFGGGPRTCIGMPLAQLEARIILASILQHYTPQPAPGYTPGFQPVITLRPKRHL
                     QVTLMPASSSDSDAQWKQFARLNAM"
BASE COUNT          529 a          664 c          580 g          488 t
ORIGIN      
        1 tgacgatgaa aaaacctgga atgtgcggaa acactgatgc atccaccaca ggaataccat
       61 caccatcacc aagctactat tggggaaccg tgcggaagct gatcgccagg cggttccagc
      121 cgttgatgga aataatggcc agcgtcaggt agaccagttc ttcgtcggta aattgcttgc
      181 gtacttctgt ataggtctcg tccggcacgt ggtcaacgga aaccagggtc acggcctccg
      241 tccatgccag ggctgctcgc tcacgctcgc tatagaacgg ggcctcgcgc caggcaacga
      301 gttcgtagat gcgctgttcg gtttcgccgc gcgaccgcgc agctttggta tgcagattga
      361 ttcaatacgc acacccgttg atctgggacg cgcgaatttt gacgagttca atgagcaaag
      421 gctcctgact ggccagattg gatgacctta ccagttggtc caggccgctc atagctctgc
      481 ccacgccctg aacgattttg ccgtaatcga gccgtggttg catttccata tttcgcctcc
      541 ttacgaaacg aacatactca tgctcgtttg taacagcagt taagatatcc ccaagaagca
      601 taacacgccc atacgcttac ggcaagggga atggttttga ggtaatctgc ccgttatcaa
      661 tcacaaccat gatgtcccaa cggagacaat ggagacaacg gagacaagtg tccccaattg
      721 agactctcat ccttaaccca ttgctatctc actacttctg ccttatgata gtgagtagtg
      781 aatcttgaca ccttgacaca attagcgcac tagcgcacta gaggaaacag caactcatgg
      841 cgacaactgc gcaagaactg cgaataacaa agacaatgct tccaccgggt ccccccaggt
      901 tgccttatat cggcaatctt ctctcgttga gacaagaccc gcttggcttc ctgcaacagc
      961 tgcaacgtac ctatggcaac atggcaacaa tctacgtcgg gaagaatccg gctgtgctgc
     1021 tattccgccc ggaacacgta cgctacgtcc tggtagaaca cccgcgcaat ttcgcgaatc
     1081 gtggcgtccg ccaggggaat agcgatggcg gtttcgcgaa tgaaggactg ctgaccatcg
     1141 atggcgacaa gcatcgccag cagcgccacg cggtacaacc ggccttccat aaaaaacggg
     1201 tcgaaggcta tggcgcgatc atggagcaat acacgcaaga gctgctcaaa acgtggcacc
     1261 ccggtgatac ggtcgatatg tcgcgagcca tgcaagcgtt gacactgcgt atcgtgagca
     1321 agtgcctctt cagcatcgac ctgtcttcgc agctgggccc gctcagtgat gcgttcgacg
     1381 gcgtcattgg cagttcgaca agcatggccg aagacctgct gaatattcgc atcgacaatc
     1441 ccattaccgg ctatggcaag cgcatggcag ccatgcgcca gctcgatatg ctgatctata
     1501 cgctaatagc acagcgccgt gatgatgacc gcgattacca ggacgtgctt tccatgctga
     1561 tgtcagccca gagcggggta gagccgggta caaaactgac cgagaaacag gttcacgatc
     1621 acatcctgac cttcctggcg gcaggtcacg aaacgaccgc gatagccctc gtctggacgt
     1681 tttacctgct ttcccaacat ccgcgggtac gcatcaaatt gcaggatgag attcgctcgg
     1741 tcctcgccgg tcgcgaacca acccttgacg acctcgccaa acttccctat ctcgattggg
     1801 tcttgagcga atcgatgcgt ctatatccac ccgcctggct gcagatgcgc ttcgtcacca
     1861 aagaatccac cattgatggc gtacgcctgc cggttgggac gctgctcatc ctgagccaat
     1921 gggtcattca tcgcttgccc gatatctggc aggacgcgga agtgttcaaa cctgagcgct
     1981 gggatcccgc aaatgagcag cagatccctc ctggagcgta cttccctttc ggaggcggcc
     2041 cgcgcacctg tattgggatg ccactcgctc agttggaggc caggattatt ctcgcgagta
     2101 tcctccagca ctacacgccg cagcctgctc ctggctatac ccctgggttc cagccggtga
     2161 tcaccctgcg cccgaaacgg catttacagg ttactttgat gcccgcgtcc tccagcgact
     2221 ctgatgctca atggaagcag tttgcacgct taaacgctat g
//
LOCUS       DASZAK010000231         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_289197, whole genome shotgun sequence.
ACCESSION   DASZAK010000231 DASZAK010000000
VERSION     DASZAK010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289197"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1039)
                     /locus_tag="VGT44_03885"
     CDS             complement(170..1039)
                     /locus_tag="VGT44_03885"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2579971.1"
                     /translation="MRLLLITRFTLLEAIRRRLFLAVIILSVLIVTAFTILLTIVVND
                     ALASPNNGTDSHLMLLGGGVIITVLVMWMLYLLSSMLTIVFSANMISGEVEAGTFAVI
                     VPKPLRRFEIVLGKWLGYALIVCIYTALMFLAFLGVIYWKTGYWPTGAPYALGTLELA
                     MLALLGITTLGSSTVPTIVNGAIALMLFIGAPTANIVQFIVQFVNPSSLDTTQNIATV
                     INLIIPTDALWHGASFYLLPSVADLVALRIGSFNTPFTSGQPMAPALLVWAALYCIAL
                     PALAALRFQHRDL"
     gene            complement(1036..2022)
                     /locus_tag="VGT44_03890"
     CDS             complement(1036..2022)
                     /locus_tag="VGT44_03890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2579972.1"
                     /translation="MTNMYKEPAIEVEGLRKVYRSLVAVDDVSFVMYRGEAFGFLGPN
                     GAGKSTVVKILTGLVAPTKGTVRILGQPVSNLEMRRRIGYLPELPTFHRWLRAKEFLE
                     FHGRLFGMRGAALDKRSREVLELVGLAGRENQKLGTFSKGMMQRIGLAQAILNRPDLV
                     LLDELVSGLDPVGQRDMRELLHQLKSEGMSIFLNSHQLADVEALCDRVAIINEGRILK
                     VGVPQSLFDESPVFEVRVDPVHTELLHRLGSIALAVQQDAEDPCVLYIEVQTNEQAAE
                     IAEVVLACGSRLYGLAPHHRSLEQLFLRTIDASRGRAPVESTQPKAIGRGLV"
BASE COUNT          523 a          588 c          580 g          435 t
ORIGIN      
        1 aaggcagccg cgtcgaggtt gctgatccac aggcaatgct tggcatcgtt ggtaacgatg
       61 aactcaatac agttgccggg gaggccaaac agcgtttgca gcgtgtcgtt gcatcactgg
      121 gtggtcaggc ctgaacgatc ataataatca aatgcaacag ataaccggcc tataaatccc
      181 tatgttggaa acgtagcgcg gcaagagcag gcaaagcaat acaatagagc gcggcccaga
      241 caagcaatgc tggcgccatc ggctgaccgg aggtaaaagg tgtgttgaag ctgcctatgc
      301 gtagggcgac aaggtctgcc accgagggca gcaggtagaa cgaggctcca tgccacaggg
      361 catcggttgg gatgatcagg ttgatgacgg tggcaatatt ttgcgtcgtg tccagggacg
      421 agggattgac gaattgtacg ataaactgga cgatattggc agtcggtgcg ccaatgaaca
      481 gcatcaatgc aattgccccg ttgacgattg ttggcacagt cgagctacct agcgtggtaa
      541 tccccaacag cgccagcata gccagctcga gcgttcccag cgcgtaggga gcccctgtgg
      601 gccagtaacc ggttttccag tagatcactc ctagaaacgc gagaaacatc agcgctgtgt
      661 agatgcacac aataagcgcg taacccagcc atttccctag aacaatttcg aagcgcctca
      721 gcggcttggg gacgattacg gcaaaagtgc cagcctcgac ttctcccgag atcatattcg
      781 cgctgaacac gatggtcagc atgctgctga gcaggtagag catccacatc accagcaccg
      841 taataatgac gcctccgcca agtagcatga gatggctgtc ggtgccatta ttcggactag
      901 ctagcgcatc attgacgacg atagtgagca ggatggtaaa tgccgtcaca atcagaacgc
      961 tcagtataat cactgccagg aagagccggc gtcggatagc ttccagcaag gtaaagcgcg
     1021 tgattaataa taatctcata ccagacctct cccaatagct ttcggttgag tggactcgac
     1081 aggcgcgcgg ccgcgcgaag catcgatcgt gcgcagaaag agctgttcca gcgagcgatg
     1141 atgtggcgct aaaccgtaaa ggcgcgagcc acacgcgaga acgacctcgg cgatctctgc
     1201 cgcctgctca tttgtctgaa cttcaatata gagcacgcat ggatcttctg cgtcctgctg
     1261 cacggcgagc gctattgagc cgagccggtg cagcagctct gtatgtacgg gatcgacacg
     1321 cacctcgaag acgggagatt catcgaacaa cgattgagga acgccgactt tcaggatgcg
     1381 cccctcgtta atgatggcaa cacggtcaca cagcgcttct acgtcggcaa gttggtgtga
     1441 attgaggaag atgctcatgc cttcggactt caactggtgc agcagctcgc gcatatcgcg
     1501 ctgaccaaca ggatcaagcc cggatacaag ctcgtcgagc agcaccaggt cgggccggtt
     1561 gagaatggcc tgcgccaatc caattcgctg catcatgccc tttgagaaag ttccgagctt
     1621 ctggttttcc ctcccggcca gccccactaa ctccaggact tcgcgactgc gcttgtccaa
     1681 tgccgcgccg cgcataccga agagccgccc gtgaaactcc aggaattctt tggcgcgcag
     1741 ccagcgatga aaggtgggaa gttctggtaa gtagccaatg cgccgccgca tttctaggtt
     1801 gctgaccggc tggccgagta ttcgtaccgt gcctttcgtc ggggcaacca gccccgtaag
     1861 tatcttcacg accgtgcttt taccggcgcc gttcggcccc aaaaagccaa aggcttcgcc
     1921 acgatacatg acgaagctga catcgtctac tgcgacaaga ctacgataga ccttccgaag
     1981 accctctact tctattgcag gttctttata catattcgtc ataagtgact caacctcttg
     2041 agcgagaccg aaccagacct cgcgtactca gccaagcgca gcagagcgcg tttcaactct
     2101 ttcgcttgta gaaattttct cgacgc
//
LOCUS       DASZAK010000232         4177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_503082, whole genome shotgun sequence.
ACCESSION   DASZAK010000232 DASZAK010000000
VERSION     DASZAK010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503082"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1418)
                     /locus_tag="VGT44_03895"
     CDS             complement(168..1418)
                     /locus_tag="VGT44_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2579973.1"
                     /translation="MKNLWYIARKDLLQSIKDRNSIFLLLIMPLVLITLIGFAFGNIF
                     GSGSSQITITVAVSNQDNGFVGKSIVQALSINSNQLEITVNQYSDPNKVKDEVANDSN
                     VNAGVVIPAGASNELTTALQNGQTPKNLVQVYSLPGSNDSRPTIVQDIVTKVLTAQIS
                     GSAAVGQVYSVCNQPGNHCAQQSIDTQTIVAAVGQASTQGGTDAVQTLTAGKAVKINN
                     FDQILPGYAVFFALFSINAVAGTILQEKEEGTFRRLLIAPVQRYALLGGKMLAQFLLT
                     LLQLAVLFTIGYFAFHLDIGNWPATILLLIGTSFAATGLGIVLVSLVKTRRQLNPVVT
                     LVVLVTSAIGGAWWPLFLEPTWMQQMAKLGITAWAMEGLNGVMLLGKDFSQVLPDVLG
                     LLVYGLICFVIATRLFRFQERAPA"
     gene            complement(1471..2289)
                     /locus_tag="VGT44_03900"
     CDS             complement(1471..2289)
                     /locus_tag="VGT44_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2579974.1"
                     /translation="MQDFTVSQTTTGPIHLPKDGRPVDVQVSNLVKTFGKIEAVRDVS
                     FTIGKGEIFGLLGPNGAGKSTTINMMCGYIPPTSGDTTIDGVSITREPMRVKRMIGVV
                     PQEIALYKDLNAMENLEFFSELYGLPKKERRERAEEVLRFVGLYDRRKDAIKTFSGGM
                     QRRVNMAIAMIHRPNFLMMDEPTVGVDPQSRENIFDTIEKLRDQGTTILYTTHYMEEA
                     ERLCNHIAIMDEGHIIAMGTLEQLLALRDQNREVQRPHGLQELFIQLTGKTLRD"
     gene            2482..3216
                     /locus_tag="VGT44_03905"
     CDS             2482..3216
                     /locus_tag="VGT44_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579975.1"
                     /translation="MRCPYCNGFNQDDKVYCTYCGRDMRIPTRPAAGQYPPVQQPRAA
                     PQAPPAPPQARQAASPQAPTYPPQRQAQAGQPARPMPPGQPAQAPRPAPQGAAPSPTQ
                     ARPQTRPGVEPAPLAAPDPPGPFPPRTLAELQALEQGALAYTVLESGTSVGRKKIVRI
                     VYPKGAPWQQVATLLKALKEQQDPQFESIVIQGQTDQRASLYAFTNGQLVFDRDVRLG
                     AAITNRYLFETGNGFDSDALRIVLNV"
     gene            3231..>4177
                     /locus_tag="VGT44_03910"
     CDS             3231..>4177
                     /locus_tag="VGT44_03910"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEV2579976.1"
                     /translation="MQIVELIRKKRDGGVLSAAEIEWLVEQYTRENIPDYQMSAWLMA
                     VYWRGMNARETSDLTLAMARSGEQLHVREVISPVVDKHSTGGVGDKVTLAVAPLVAAC
                     GVAVGKMTGRGLGHTGGTVDKLESIQGFRSAISRDEFMRILTAHHIVLAGQSNDLAPA
                     DGKMYALRDVTGTVESIPLIAASIMSKKLAIGASHLLLDVKVGSGAFMKSLDQARALA
                     QVMVDIGQLAGVHTVASITSMEQPLGRAVGNALEMAEAIAILHGEGPPDVSELCYHEA
                     AELLTMTGSAQDDAMRRVEQAVRSGSALAKLAEVIAAQGG"
BASE COUNT          880 a         1230 c         1238 g          829 t
ORIGIN      
        1 gacctgccca aacgcgaaat caccgagcag gtgctgcgtc tgctagaaaa gacgaaatcg
       61 aacgcgcaac tggtagaagt ggtgcttcaa caattataga tgggagacag acaaagcgat
      121 ggcaggccga cagtctgcca tcgctttgtc tgtctcccat ctataattta cgctggcgct
      181 cgctcctgga agcggaacag gcgcgtggca atcacgaagc agatcaggcc atagaccagc
      241 aggccaagca catcgggcag cacctgcgag aaatccttgc cgagcagcat caccccgttc
      301 agaccctcca tcgcccaggc ggtgataccc agctttgcca tttgctgcat ccaggtcggc
      361 tccaggaaga gtggccacca cgcgccgccg atggccgatg ttaccagcac caccagcgtg
      421 acgaccggat tgagctgcct gcgcgtcttg acgagcgaga cgaggacgat gcccaggccg
      481 gtcgccgcga agctggtgcc gatcagcaaa aggatcgtcg ccggccagtt gccgatgtcc
      541 aggtggaagg cgaagtagcc gatagtgaac aaaacggcca gttgcagcag cgtcagcagg
      601 aactgcgcca gcatcttgcc tcccagcagc gcgtagcgct gtaccggcgc gatcagcagg
      661 cggcggaagg tgccttcctc tttctcctgc aagatggtac ccgcgacggc attgatgctg
      721 aagagcgcga agaagaccgc gtaacccgga aggatctgat caaagttgtt gatcttcacc
      781 gctttgcccg ccgtcagcgt ctgcaccgcg tccgtcccgc cctgtgtgct ggcctggccg
      841 acggcagcaa cgatggtttg cgtatcgatg ctctgctgcg cgcagtggtt gccgggctgg
      901 ttgcacacgc tatagacctg gccgacggca gccgaacctg agatctgcgc ggtaagcact
      961 ttcgtcacaa tgtcctgaac gatggtcgga cggctgtcat tactgccggg cagcgaataa
     1021 acctgcacga gattcttcgg cgtctgtcca ttctgcagag cagtggtcag ttcgttgctt
     1081 gcgccagccg ggatcactac gccggcattg acgttgctat cattagccac ttcgtctttg
     1141 accttgtttg ggtcgctgta ttggttgaca gtaatttcga gctgattcga gttgatcgat
     1201 aatgcctgca caatcgattt gccgacaaag ccattatcct ggttgctcac tgcaacggtg
     1261 atagtgatct ggctggagcc gctgccgaaa atgttgccaa aggcgaagcc gatcagcgta
     1321 atcagcacta gcggcataat gagcaggaga aagatcgagt tgcggtcttt gatcgattgt
     1381 aagaggtctt tgcgcgcgat ataccagaga tttttcattg aagttgccct ttcctgtata
     1441 atatcgaaat gtactcaagc agtctgccgg ctagtcgcgc agcgtcttgc cggtcagttg
     1501 gatgaaaagt tcttgcaatc catgtggtcg ctgcacttcg cggttctgat cgcgcaaggc
     1561 gagcaactgc tcgagcgttc ccatcgcgat gatgtggcct tcgtccataa tcgcaatgtg
     1621 gttgcagagc cgttcggctt cttccatgta gtgggtcgtg tagagaatgg tggttccctg
     1681 gtcgcgcagc ttttcgatgg tatcgaagat gttctcgcgc gattgcggat cgacgccgac
     1741 ggtcggctcg tccatcatca ggaagttggg ccggtgaatc atcgcgatag ccatattaac
     1801 gcggcgctgc ataccacctg agaaggtctt gatggcatct ttgcgccgat cgtacaggcc
     1861 cacaaaacgc agcacttcct ccgcgcgctc gcggcgttcc tttttcggca atccgtacag
     1921 ttcgctaaaa aattccaggt tctccatcgc gttcaaatct ttgtagagcg cgatttcctg
     1981 tggcacgacc ccgatcatgc gcttgacccg catcggctcc cgcgtgatcg agacgccatc
     2041 gatcgtggta tcgcccgatg tcggcgggat atagccgcac atcatattga tggtcgtgct
     2101 tttcccggcg ccattcggac caagcaggcc gaagatctcg cctttgccaa ttgtgaatga
     2161 aacatcacgc acagcctcaa ttttgccgaa cgtcttcacc aggttcgaca cctggacatc
     2221 caccgggcgg ccatccttcg gcaggtgaat ggggccggtc gttgtttgcg atacagtaaa
     2281 atcctgcata agttctcctc cgtcagtacg ctcttgacta acgagcgcat tgctcgttta
     2341 tccttacagg aaagagtata gttgaaatgg cgcacggatg tggagtacga aaaggcacgc
     2401 tctgcgctat gacgaaagtc atatgcaagt gctatacttt agcagtgtca ggcagttgaa
     2461 caagagacgg gagagtcggt tatgcgttgt ccctactgta atggatttaa ccaggacgat
     2521 aaagtctatt gtacctattg tggccgcgat atgcgcattc ccacgcgacc agccgctggc
     2581 cagtacccgc cggtgcagca gccgcgcgca gcgccacagg cgccaccagc gccgccacag
     2641 gctcgtcagg ccgcgtcgcc acaggctcca acctacccac cgcagcgcca ggcgcaagcg
     2701 ggacagccgg cgcggccaat gccgccagga caaccggcac aagcgcctcg cccggctccg
     2761 cagggcgcag cgccttcacc cactcaggcg aggccgcaga ccaggccagg cgttgaaccc
     2821 gccccactag ctgcgcccga tccacccgga ccgtttccgc cgcgcacact tgcagaactc
     2881 caggctttgg agcagggcgc attggcgtac acggtacttg agagcgggac gagcgttgga
     2941 cgtaaaaaaa ttgtgcgcat tgtctatcca aaaggcgcgc cctggcagca ggttgccacc
     3001 ttgctcaaag cattgaaaga gcagcaggac ccacagttcg agagcatcgt tattcaaggc
     3061 cagacagacc agcgcgctag cctttacgcc tttaccaacg gccagcttgt gtttgatcgc
     3121 gatgtgcgcc tgggcgcagc aatcaccaac cgctacctgt tcgagaccgg caacggcttc
     3181 gatagcgacg cgctgcgcat tgtcctcaat gtctagataa aggaacgccc atgcagatcg
     3241 tggaactcat ccgcaagaag cgcgacggag gggtgcttag cgctgccgag atcgaatggc
     3301 tggtcgagca gtatacacgc gagaacatcc ccgactacca gatgtcggcc tggctgatgg
     3361 cggtctactg gcgcggcatg aacgcgcgtg agacgagcga tctgacgctg gcgatggcgc
     3421 gctctggcga acaactgcat gtgcgcgagg tcatctcgcc ggtagtagac aagcacagca
     3481 ccggcggcgt gggcgacaag gtgacgctgg cggtcgcccc cctggtggcg gcctgcggcg
     3541 tagcggtggg caagatgacg ggacgcggcc tggggcatac cggcggcacc gtcgataagc
     3601 tcgaatcgat ccagggcttt cgttccgcca tcagtcgcga cgaattcatg cgcatcctga
     3661 ccgcacacca tatcgtgctg gccggtcaat cgaacgacct ggcgcccgcc gatgggaaaa
     3721 tgtacgccct gcgcgacgtg accggcacgg tcgagagcat tccgctcata gccgccagca
     3781 tcatgagcaa gaagctcgcc atcggcgcat cgcatctcct gctcgatgtc aaggtgggtt
     3841 ccggcgcgtt tatgaagagc ctcgaccagg cgcgcgcgct ggctcaggtg atggtggata
     3901 tcgggcagtt ggccggggta cataccgtcg cgtcaatcac ctcgatggag cagccgctcg
     3961 gacgcgcggt aggcaacgcg ctggaaatgg cggaagccat cgccatcctg cacggcgaag
     4021 gccccccgga tgtctcagaa ctgtgctatc acgaggcagc cgagctgctg actatgacgg
     4081 gaagcgcgca agatgatgcc atgcggcgtg tcgagcaggc ggtacgatcc ggctcggccc
     4141 tcgcgaagct ggccgaggtc atcgcggccc agggcgg
//
LOCUS       DASZAK010000233         2451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_182546, whole genome shotgun sequence.
ACCESSION   DASZAK010000233 DASZAK010000000
VERSION     DASZAK010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182546"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..570
                     /locus_tag="VGT44_03915"
     CDS             220..570
                     /locus_tag="VGT44_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579977.1"
                     /translation="MRIWQLRAGASAAELETLAATGIAEMHRWIPGIERLALVRVEPG
                     KKDSSPCYLVLTTFTNHQAYLAWRMIEEEGPDYWERYASVFMYWEQIVRLVEEYSGDL
                     IDLQQSDKPQTTPG"
     gene            584..2410
                     /locus_tag="VGT44_03920"
     CDS             584..2410
                     /locus_tag="VGT44_03920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEV2579978.1"
                     /translation="MSQNYGVLRVVRVKPARSSAGSPNTQKYSTGWLPEEGLVHVLSR
                     TDTSIGRALNNDIILMDPTVSREHARLALDETGWHIINLTARNVVRVNGRPVPSGGSL
                     PMQPQDVLVLGSTMLQLIAPNLPAQGQNDAPLAQRNGVSSDPISDKPFASAAVPSSPR
                     TGQDQAQGIAILGTHAVSIPDQPDAEQEAQAWNEEEEVSVLGAGVTMQFALPQRMGLG
                     TRWLIAGIGIAILAVSAMITIILNSTVGLSALSQNGAASIIAALTIPLIPAVGIGLLV
                     NFMDRYEREPWFLRLSAFLWGALIAIPPALFIEQNIDTFIQNTFDPNNTNDIMRILLQ
                     GLNAGVTEETIKGLGLLLLFFILRDEFDNVTDGIVYGALIGAGFAMVENFNYFALNSK
                     NFLVFLIVGRIVLGWLGHSTFIACFGAALGYVRHTKVRWKQIVIPLAGFLIAVGLHSF
                     FDFVDFQASAAIRTSSENPSVVNFALFAIIANYIPPFLAQIGLLYILMRSLAHEAAVI
                     REFLAVEVSTGVVQVREYALLQNSFQRTREERRVLRAYGVKQWLRVKTLYQTEIGLAF
                     RKWHVSMGDKPKLGYLQPEDAYRQRIRRLRQEIRAAEAKVSQ"
BASE COUNT          511 a          689 c          708 g          543 t
ORIGIN      
        1 ccgtggattg gccgggctac gaggctgcgc tggaacgaca ccgcgaagtg agccgcgcca
       61 gcgcggaacg ccattttcag tagcggtttt ttccacttgt caatcgcttc attcccatgt
      121 atattgatag cagttcaaag atgccatact cttttaactt cgaaacttcc aatacgtgac
      181 ataaattgac tgcgaggaac tatgggtgag tattgctctg tgcgcatctg gcagctcaga
      241 gcgggggcct cggctgccga acttgagacg ttggcggcca ccggtatcgc agagatgcat
      301 cgctggattc caggaataga gcggcttgca ttggtgcgcg ttgagccagg taagaaagat
      361 agttcgccgt gctacctggt gcttaccacg tttacgaacc atcaagcgta ccttgcgtgg
      421 cgcatgatcg aagaggaagg gccagattac tgggaacggt acgcctcggt gtttatgtat
      481 tgggaacaaa tagtacgcct ggtcgaagaa tatagtggcg atttgattga tctacaacaa
      541 tctgataagc cccagaccac tccaggatag ccaggataat cgtatgagtc aaaactatgg
      601 ggtattgcgc gtggtgcgcg tgaaaccggc gcgttcgtct gccggttcgc ccaataccca
      661 gaaatactcg acaggctggc tgcctgaaga agggctggtt cacgtccttt cgcgaacaga
      721 tacctcgatt ggtcgtgcct tgaataatga cattattctg atggacccaa ccgtctcacg
      781 tgagcacgcg cgcctggcgc tggatgaaac tggttggcat atcatcaatc tgacagccag
      841 gaatgtcgtg cgcgtcaatg gccgccccgt tcccagcgga ggcagtctgc ccatgcagcc
      901 gcaggatgta ttggtgctgg gcagcacgat gttgcagttg atagcgccca acctgccagc
      961 gcagggtcag aatgacgcac cgcttgcgca acgcaatgga gtgagcagcg atcccatcag
     1021 cgacaaaccc ttcgcttccg ctgctgtccc atcatctccc cgcaccggtc aggaccaggc
     1081 gcagggcata gcgatactcg gcacgcacgc ggtatccatc ccagatcagc ctgatgctga
     1141 gcaggaagcc caggcgtgga acgaggagga agaggtcagc gtgctgggcg cgggcgtgac
     1201 gatgcaattt gccctgccgc agcgtatggg cctggggacg cgctggctca tcgcgggcat
     1261 cggcatcgcg attctggccg tcagcgccat gattaccatt attctgaata gcacagttgg
     1321 actttccgcg ctctcccaga acggcgcggc cagcatcatt gccgcgctga ccattccgct
     1381 tattccagcc gtcggcatcg gcctgctggt caattttatg gatcgctacg aacgcgaacc
     1441 ctggtttttg cgcctgtcgg cctttctgtg gggcgcgttg atcgccattc cgccggcgct
     1501 gttcatcgag cagaatatcg acacgttcat ccagaatacc ttcgatccca acaataccaa
     1561 cgatatcatg cgcatcctgt tgcagggctt gaatgccggt gtaacggaag aaacgataaa
     1621 ggggcttgga ctgctgctgc tgttcttcat cctgcgcgac gaattcgaca atgtgaccga
     1681 tggcattgtc tatggcgcgt tgatcggcgc gggcttcgcg atggtggaga acttcaacta
     1741 tttcgcgctg aactcgaaaa acttcctggt gtttctgatt gtcggcagga tcgtgctggg
     1801 ctggctcggc cactcaacct tcatcgcctg tttcggggcc gcgcttggct acgtgcgcca
     1861 tacaaaggtg cgctggaagc agattgtgat cccgctcgcc ggttttctta tagccgtagg
     1921 actccatagc tttttcgatt ttgtggattt ccaggccagc gccgcgattc gcacatcgag
     1981 cgagaacccc tcggtcgtca attttgccct gtttgctatt atcgccaact atatcccgcc
     2041 cttcctggcg caaatcggcc tgctctacat cctgatgcgc tccctggcgc acgaggccgc
     2101 cgttattcgc gaattcctgg cggtggaggt cagtaccggc gtggtgcagg tgcgcgaata
     2161 tgccctgctg caaaactcct tccagcgcac gcgcgaggag cgccgcgtcc tgcgcgccta
     2221 tggagtgaaa caatggctgc gcgtcaagac gctctaccag accgagatcg gcctggcgtt
     2281 ccgcaaatgg catgtgagta tgggcgataa acccaagctg ggttacctcc aacctgagga
     2341 tgcctaccgc cagcgcatca gacgcctgcg ccaggagatt cgcgcggctg aggcgaaagt
     2401 aagtcaatga gacgaaagca agcggcctgc gcaatccgcg ggattgcgca g
//
LOCUS       DASZAK010000234         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_361219, whole genome shotgun sequence.
ACCESSION   DASZAK010000234 DASZAK010000000
VERSION     DASZAK010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361219"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(401..2326)
                     /locus_tag="VGT44_03925"
     CDS             complement(401..2326)
                     /locus_tag="VGT44_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579979.1"
                     /translation="MSSIADGTVRIQTSSQSLAAPPSWFGEVVLLSASLRQHGVLTKI
                     SEQVRFARRRFGHDEVIDFLAILFGYAISGERTLEAFYQRLQPVAVPFMALFEREQLP
                     ARSTLSRFLAALTQEPVEALRTLFLADLLARPLTPDKQTGGLVDRKGDTWMIFDIDGT
                     REAARQRALPQTDELPAPFRRLDDVCAPGYRGRKRGEVVRTRTTIAQAHSYQWLGSFG
                     NRGNGHYREELRKGLSAIRRYLTASQLEPARTLLRLDGQYGTGAALSDVAEFAFVTRG
                     KEYSVLDHPLVQARLHLPPDQSQQRPESQMVRSLYDCPQIPVGPDGAACRVIVATHPA
                     GKKKSPVGVTRAGVVYELFFTKLPQHAFTACDVVELYLHRGAFEPQLSDEDQEIDPDR
                     WCSHSAWGQEAWSIIGQWVWNLRLELGHQLQPDPVRTTEFAAAIPPQNEQSANSPPSS
                     PPASGYGPPTPAASWKTGRFTGADFPLQPDGTLRCPAGQPLHLQERRREPDASLRLVY
                     AASIRHCRSCPLREQCQWNGSATAKPRQVSLLLYPLRVGLAPLLWRDWRRRDHRRACM
                     QLTRQQRLEVSLSPPTVTSPPTADVILSRAQRARSRLSWPERLARNARASTAGQVTIR
                     VFGVPAAFAASLSLATA"
     gene            2415..3092
                     /locus_tag="VGT44_03930"
     CDS             2415..3092
                     /locus_tag="VGT44_03930"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2579980.1"
                     /translation="MRIAWDLQARDLVPEVRLVDSGYVSGEVLALHAELGIELVGPLK
                     QEGGWQHETGYGMSAFQVDWQQQQVRCPQGHLSQNWCPGRHNRGEEVIRVSFSAVTCH
                     ACPVKALCTKRQNSKGRILTLSPQPIAEARSRRRTEQATPAFQQRYALRAGIEGTLSE
                     GIRSHGLRRARYRGQPKTQLQAKAIAAAINLVRIRQMLQRTALGLPPRPKRPLSSFAR
                     LRSLLAP"
     gene            complement(3224..>3511)
                     /locus_tag="VGT44_03935"
     CDS             complement(3224..>3511)
                     /locus_tag="VGT44_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579981.1"
                     /translation="LTGDPLGEPDFPSEGDRVIRWLTGGSMPDAWVTFHPEGELIAVR
                     CAGCRAIVGHLPADLFAKDEIVRQVGAIREAGHQHDKPTDWPRTKVIAEAN"
BASE COUNT          723 a         1019 c         1120 g          649 t
ORIGIN      
        1 ataatcgaac caggtattcc ctcgtgataa cacaatccat acggcaggag gcattccatt
       61 cgggagagga cattctaaac accccggtgc ctcaaccttt ttctcgaatt gcaactctgg
      121 tgtgccgctg atctggcacc ttacacccgt aagattcgta ccatctatat cccacgcgat
      181 gcgcaacgag ctggttgaag agttcatcgt ggctcggtga gcaaattcct gctgaatcgg
      241 agggggagag cggaagatcg ggacaaagtg gcgagatcga gagcaaaacc ggtagcagaa
      301 gggaagaagg gagacccagc cgggacaaga gggcagcagg ttccagccgg agaagccagc
      361 ttctccgaga agagggaata tcatggcggt caaggccgtg ttacgccgtc gccaagctga
      421 gcgaggccgc aaaggcggcc gggacgccga acacccggat ggtgacctgc ccagcggtgg
      481 aagcacgagc attgcgagcc agccgctctg gccacgagag acgagaccgc gcgcgttggg
      541 cgcgcgacag gatcacatcg gcggtgggcg gcgaagtgac ggtgggtggc gacaggctga
      601 cctcgaggcg ctgctgtcgg gtgagctgca tgcaggcgcg ccggtggtct ctgcgcctcc
      661 aatcgcgcca gagcagtggg gcgagaccga cgcgaagcgg atacaacagc aggctgacct
      721 ggcgtggctt tgcggtcgcg ctgccattcc attgacattg ctcacgcagc ggacaggagc
      781 ggcagtggcg aatgctggct gcatacacca ggcgcagact ggcatcaggc tcgcgacgtc
      841 gctcctggag gtgaagcggc tgcccggcgg gacagcgcag tgtcccatca ggctggagag
      901 gaaagtcggc tccggtgaac cggcccgttt tccaggaggc ggcgggagta ggtggcccat
      961 atcccgaggc gggaggggat gagggcggag agttggccga ctgctcgttc tgcggtggga
     1021 tggcggcggc aaattcagtc gtgcgcaccg ggtcaggttg aagttgatgt cccaattcca
     1081 ggcgcagatt ccagacccat tggccgatga tactccatgc ctcttgcccc caggcggagt
     1141 ggctgcacca acgatccggg tcgatctcct gatcttcgtc cgaaagctga ggttcaaagg
     1201 caccgcgatg caggtacaac tcaaccacat cgcaggccgt gaaggcgtgt tgcggcagtt
     1261 tcgtgaagaa cagttcgtag accacccctg cgcgtgtgac cccaacggga ctcttctttt
     1321 tgcccgcggg atgggttgcc acgatgacac ggcacgctgc gccatcgggt cccactggga
     1381 tctgggggca atcgtagagg ctgcgcacca tctggctttc cggacgttgc tgagattgat
     1441 cgggtggcag gtggagacgt gcttgaacca gaggatgatc gagcacgctg tactctttgc
     1501 cgcgagtcac aaacgcgaac tcagccacat ccgacagcgc ggctccggtg ccatattggc
     1561 cgtcaagccg cagcagcgtg cgcgccggtt cgagctgaga cgccgtgaga tagcgacgaa
     1621 tggcagagag acccttgcgc agctcttccc ggtaatgccc gttgccccga ttcccgaaac
     1681 tgccgagcca ctggtagcta tgggcctgag caatcgtggt gcgcgtgcga accacctccc
     1741 cgcgtttgcg gcctcggtag ccaggggcgc agacatcatc caaccggcga aagggcgcag
     1801 gaagctcatc agtctggggt aaggcgcgtt gtcgggctgc ctcccgtgtc ccatcaatgt
     1861 caaagatcat ccaggtgtcg ccttttcgat ccaccagccc gccggtttgc ttgtcaggcg
     1921 taagggggcg agcgagcaga tcagcgagaa agagcgtacg cagcgcttcg accggttcct
     1981 gcgtgagtgc cgctaaaaag cgcgagaggg tcgagcgagc aggcaactgc tcccgctcaa
     2041 acaaggccat gaagggaacc gcaacgggct gcagccgctg gtaaaatgcc tccagggtgc
     2101 gttccccact gatggcatac ccgaagagga tggcaagaaa gtcaatcacc tcgtcgtgcc
     2161 cgaaccggcg gcgagcaaag cgcacctgct cactgatctt cgtcaggact ccatgctggc
     2221 ggagagatgc cgagagcagc acgacttctc caaaccacga cggcggtgcg gcaagggact
     2281 gcgaggaagt ctggatacgc accgttccat cggcgatact ggacatatgt gcctccttga
     2341 aagatggaag ttggttcttc cttctttcaa ggaggcacat ttttatcctc gcttcctgcg
     2401 ggttaggcaa ctcgttgcgc atcgcgtggg atttgcaagc tcgcgacctg gtgccagagg
     2461 tgcggctggt cgatagtggc tatgtcagtg gcgaggtgct cgctctgcac gccgaactgg
     2521 ggattgagct ggtgggtccg ctcaaacagg agggaggctg gcaacacgag acgggctatg
     2581 gaatgagcgc gttccaggtg gattggcagc agcagcaggt gcgctgtccc caaggacacc
     2641 tctcgcaaaa ctggtgcccc ggacgacaca accgaggcga ggaggtgatc cgggtgagct
     2701 tttctgcggt cacctgtcat gcctgtccgg tcaaagcgct gtgtaccaag aggcagaaca
     2761 gtaaggggag gatcttgact ctttcgcctc agcccatcgc tgaggcgcgc tcgcgccgcc
     2821 gaacggaaca agccaccccg gcctttcagc aacgctatgc cttgcgggca ggcatcgaag
     2881 gcactctttc ggaagggatt cgcagccatg gtctgcgccg ggcccgctat cgaggacagc
     2941 caaagacgca gctgcaagcc aaagccatcg ccgccgccat caacctggtt cgcatccgtc
     3001 agatgctgca acgcaccgct ctgggactcc cgcctcggcc caagcgtcct ctatcatctt
     3061 ttgcacggct ccgatcactc ttggcgccat gatgtccgga caaaatttcc cagcagagtc
     3121 caccaatgtt tgtattgggc ctatttagcg aatgcaccac taaggatgat ctatgctgga
     3181 gataggtatg gggacccggt tccgttgggc ttgcccgcca tcgctagtta gcttctgcta
     3241 tcacctttgt gcgaggccag tccgtaggct tatcatgttg atgcccggcc tctcgaatcg
     3301 ctccgacttg cctcacgatt tcatcttttg cgaataggtc tgccggcaag tgaccgacaa
     3361 tggctcggca acctgcgcaa cgcactgcga tcagctcgcc ctccggatga aatgtgaccc
     3421 atgcatccgg catggagccg ccggtgagcc agcgtattac acggtcgccc tcggatggga
     3481 agtcaggttc accaaggggg tcgccggtga g
//
LOCUS       DASZAK010000235         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_254739, whole genome shotgun sequence.
ACCESSION   DASZAK010000235 DASZAK010000000
VERSION     DASZAK010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254739"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VGT44_03940"
     CDS             <1..180
                     /locus_tag="VGT44_03940"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579982.1"
                     /translation="LKRGSNAALIPGRQMQVCAGDASIGQFGEIHPEVLTNWELFYPA
                     SFIELDLDEISAHRS"
     gene            350..1510
                     /locus_tag="VGT44_03945"
     CDS             350..1510
                     /locus_tag="VGT44_03945"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2579983.1"
                     /translation="MTQRIALYWHNGRSLGHTVRSATLGQALLQTMPGCAVAGMTGAS
                     RGLELLPAGMDVVKLPSYLAFDNQQGATFAPVLPIEQEQFYRIRASLVEAFIREYQPH
                     VFLVDYYPQGKAGELIPAITGAWQTRNVLGLRGILGTPEETNSQVFHREMERFLLAHY
                     AAIHVYIDERVFCLEEYYDVPRSLRSLITYTGYVTRSGAIAKEDARAQLGVERGARVV
                     VASFGGGQGTEALWQALLNGLERIAPRFDLAYFAAGPYLEDAAYERLQRRIALHPDWT
                     WTRLLDPLPTLMAASDLFIGSGGYNTLAEVMAVNANALIVPRQLQEREQALHAARLAK
                     LGIVRVAELETMLQSDSSALLEMCLREPYPAGNMRIATDGARRNAQLMGLLM"
     gene            complement(1522..2061)
                     /locus_tag="VGT44_03950"
     CDS             complement(1522..2061)
                     /locus_tag="VGT44_03950"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEV2579984.1"
                     /translation="MTAFVALFRGINVGGRVVKMNDLKDLHASLGFKDVLPYIQSGNV
                     VFTSDEADEAQIRRHIEESFEHKFGYHVEVLVRTSAEISEIIQKNPFQNQPDKESNWV
                     VVLFLAARPGDTAWQDLVTTYTGPEELYITGKEVYIYYSGGIGRSKLTLNLMEKKLKT
                     VGTARNWNTVLKLRELLSR"
BASE COUNT          403 a          607 c          675 g          494 t
ORIGIN      
        1 ctcaaacgcg gcagtaacgc cgcgctgatt cctggtcggc agatgcaggt atgcgcgggg
       61 gatgcttcga tagggcaatt tggcgagatc catccagagg tgctgacgaa ctgggagttg
      121 ttctatcccg ctagctttat tgagcttgac ctggacgaga tttctgctca ccggtcgtag
      181 ggacgcgatt catcgcgtcg tggcggctat gtcatcctga gcgcagcgaa ggatctgatg
      241 cgccggggac cgagatcctt cgctgcgctc aggatgacat actgttaccg atcttgtttg
      301 tgaacattca tcatcgcgtc cctacgaccg gtgtgtttgg aggatactat tgacgcaacg
      361 gatcgccctg tactggcata atgggcgcag tctgggacat acggtgcgca gcgcgacgct
      421 tgggcaggcg ctgttgcaaa cgatgccggg ctgcgcggtt gccggtatga cgggggcgtc
      481 gcggggcctc gaactgctcc cggctggcat ggacgtggtg aaattgccgt cctacctggc
      541 gtttgataac cagcagggcg cgactttcgc gccggtgctg ccgattgagc aggagcagtt
      601 ctatcgcatt cgcgccagcc tggtagaggc attcattcga gaataccagc cgcatgtgtt
      661 cctggttgat tactacccgc agggcaaggc gggcgaactg atcccggcta ttaccggcgc
      721 ttggcagacg cgcaatgtgc tggggctgcg tggcatcctg gggacgccgg aggagacgaa
      781 tagccaggtc tttcaccggg agatggaacg ctttctactg gcgcattatg ccgctatcca
      841 cgtctatatc gatgagcgcg ttttttgcct ggaggagtac tacgacgtgc cgcgctcgct
      901 gcgttcgctc atcacctata cggggtatgt gacgcgctca ggagctatcg caaaggagga
      961 cgcgcgcgcg cagcttggcg tcgagcgtgg ggcgcgcgtc gtcgtcgcca gtttcggcgg
     1021 cgggcaggga accgaggcgc tctggcaggc gctgctcaat ggcctggaac gtatcgcgcc
     1081 gcgcttcgac ctggcatact ttgccgccgg accatacctg gaggatgctg cttatgagcg
     1141 tttgcagcgg cgcattgccc tgcatcctga ctggacatgg acgcgcctgc tcgatcccct
     1201 gccgacgttg atggcggcaa gcgacctctt catcggctcc ggcggctaca atacgctggc
     1261 cgaggtcatg gcggtcaacg ccaacgcgct gatcgttccc cgccagctcc aggagcgcga
     1321 gcaggcgctc cacgccgcac ggctggcgaa gctgggcatc gtgcgcgtgg cggagctgga
     1381 gacgatgctg caatcggata gttcggcttt attggagatg tgcctgcgcg agccgtaccc
     1441 ggcaggcaat atgcgaattg cgacggatgg cgcgcggcgg aacgcgcagt tgatggggct
     1501 gctcatgtaa atcgacgtag cttagcgcga gagcagttcc cgcaatttga gaacggtgtt
     1561 ccaatttcgt gcggtaccaa ctgttttcag ttttttctcc atcaaattca gagtgagttt
     1621 tgagcgtcca atgccgccgc tataatagat atacacttct ttgccggtta tgtatagttc
     1681 ctcggggcca gtataggtcg ttaccaggtc ttgccatgcg gtatcgccgg ggcgcgctgc
     1741 cagaaacaga acgaccaccc aatttgactc tttgtctggt tggttctgaa acgggttttt
     1801 ctgaatgatc tcgcttattt cggctgacgt gcgcacgagg acttccacgt gatagccaaa
     1861 tttgtgttca aagctctcct caatgtgcct gcggatttgt gcctcgtctg cttcatcgct
     1921 ggtgaacacc acatttccac tctgtatgta gggcaggaca tccttgaagc ccagcgatgc
     1981 gtgcaaatcc ttgaggtcgt tcatttttac gacgcgacct ccaacattga tgccacggaa
     2041 cagtgctaca aacgctgtca tatacaagcc tcttccttca atcagatgag cgcccggcca
     2101 gggcgatggc cagcatcgcc cctaccctct cccaacccgc gtgagaagcg gttgcgcgag
     2161 ggtaggggcg atgctggcc
//
LOCUS       DASZAK010000236         6279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_289257, whole genome shotgun sequence.
ACCESSION   DASZAK010000236 DASZAK010000000
VERSION     DASZAK010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289257"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VGT44_03955"
     CDS             complement(<1..842)
                     /locus_tag="VGT44_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579985.1"
                     /translation="MNKPSESPDANGQATTAPARPRAAAKKAEPGTQTTSASKEVGAK
                     TAAAQRAPSQATASATGKATATTPATAARPPRATERASSAPAAPRPTAPAAQQSAATA
                     PRPTAPAAPRPTAPAAQQSVATGQRLAAPNAQQSAATAARPAGTSRPGAGPAPRVANR
                     NSGTPAAPSRTQTTQGGQNAQHSQRPTTTAHPTSPARPPVRSGASHPGAGRGGRPGGG
                     GRPAARPAAPSERRVSAVKEKPTGPISLPAQIVVKDLADLLHATPNEVISDLIKKHGI
                     FANI"
     gene            complement(1257..1559)
                     /locus_tag="VGT44_03960"
     CDS             complement(1257..1559)
                     /locus_tag="VGT44_03960"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV2579986.1"
                     /translation="MAKAIKQQGKRPKHIPMRTCVVCKQTRPKRELLRVVRTPDGHVL
                     LDAGGKKSGRGAYLCARLSCWETALKKKRLEQEFEVGISEEDRAGLDAFVATLPQD"
     gene            complement(1581..2846)
                     /gene="nusA"
                     /locus_tag="VGT44_03965"
     CDS             complement(1581..2846)
                     /gene="nusA"
                     /locus_tag="VGT44_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEV2579987.1"
                     /translation="MRSEFQAAIAQLIAEKGLPREVVMETVANALLAAYRKSYGDGEN
                     IRIEVDKNGEVRVWSARRVVAQVADPNDEISLAEAQRQVPHAALGQTIDVDSSYIFNK
                     IPAQTAKQVILQRIREAEHEYLYEQLKDWTGEIRLGVLTRRDPVRGWLLDFDKAERVE
                     GVMPPGEEVPTEKYRIGQRLRVYVYDVRRVQGRMLQIMVSRTHRDLVRRLFEQEVPEI
                     YEGTVEIKAIAREPGSRSKVAVVARQEGLDPIGSCVGVRGSRINNIVHELNDEKIDII
                     QWSPDQATFVSNALSPVKPFKVQLRDSDHTALVTVAERQLSLAIGKDGQNARLAAKLT
                     GWRVDVTKPAEGETFEETLDERHEPAAAYPGRRDRNERSGDQGGRGPAGRNDRRDRHD
                     RGGDRDRVKRSAPYQRGSGRYSGPVEEDD"
     gene            complement(3054..4313)
                     /locus_tag="VGT44_03970"
     CDS             complement(3054..4313)
                     /locus_tag="VGT44_03970"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEV2579988.1"
                     /translation="MKKLLAMVARQSRRFSIRRLLLQIFGLMVGLQSSIVLILQVVSI
                     RRKRHQPQGRFPHGHLERVPVGENVLQLHDFGRDLYNDMLAAIDEAKESIYLETYIWK
                     GDEVGQAFKEHLARKAAEGVEVYVIFDQFANMVVPSKFKVFPASIHTLPYYAIGSPLH
                     LFDPRRYALDHRKLLVVDGATAFIGGYNIGKVYATMWRDTHLRIRGSVAADLAQSFTD
                     FWNLHHKPPQRITRHYPRHFDPLINIHETNALRLTFPIRDMYIEAINRAEQRILLTDA
                     YFVPDRVLIDALKSAAERGVDTRVLIPWTSNHIITDWLARGNFTECLRANVRIFGYRH
                     MLHAKTCSIDGQWSTIGTANLDRLSSVGNYEINAEVYSEQLACQMEELFERDLSNAFE
                     ITLDTWLNRSWYMKLSERILSPLRLLM"
     gene            4455..5597
                     /locus_tag="VGT44_03975"
     CDS             4455..5597
                     /locus_tag="VGT44_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2579989.1"
                     /translation="MAVSHLSEEEQGLIEAIREIALERVAPRAAEIDHTGQFPWDMKE
                     LLAQQDILAMPFPVDYGGIGASELAVVMAIEELARHCATTALILAVQQLGSLPIMLGG
                     NEQQKGKYLPRLASGEWTAAFGLTEAGSGSDAAAMTTVAVRKGDKYLLNGSKRFITNG
                     GLAQVNSIFALTDTTVGTRGISAFIVERDFPGFSVGRIEDKMGIKGSQTAELIFTDCE
                     VPAENLLSKEGDGFKIAMMTLDRTRPGIGAQALGIAQGALDLAVSYSKQRKQFGKPIA
                     DNQGIQFMLADMATKVEAARLLVYNVAEMIANGEQHFSKYSAMAKMYASDIAMDVTND
                     AIQILGGYGYMKEYPAERMMRDAKITQIYEGTNQIQRLVIARDLLM"
     gene            5610..>6279
                     /gene="prs"
                     /locus_tag="VGT44_03980"
     CDS             5610..>6279
                     /gene="prs"
                     /locus_tag="VGT44_03980"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV2579990.1"
                     /translation="MREITIFSGTAHHELADEVCRYLHVPLCKSITRHFGNDCLYVQL
                     NANCRESDVFLIQPLVSPVQEHLMELLFMLDASRGASAARTTAVIPYYAYARSDKKDE
                     PRISIAGRLVADLLSTAGANRVLTMTLHAPQVHGFFSVPTDHLNALKVLAGHFRNHNL
                     RNTVVVSPDLGNAKSASQFARLLRVPVAAGSKQRLSDDQVVIDTIVGDVEGKDVIILD
                     DEIAN"
BASE COUNT         1174 a         1856 c         1888 g         1361 t
ORIGIN      
        1 ttgatattgg caaagatgcc gtgcttttta atcaggtcgc tgataacctc gtttggcgta
       61 gcgtgcagca gatccgcgag atctttgacg acgatctgag ccggtaacga gatcggaccg
      121 gtcggctttt ccttgacagc gctgacacgc cgctctgatg gggcggcagg acgcgctgcc
      181 gggcgccccc ctccgcccgg acgcccgccg cgtcccgcgc cgggatgtga cgcaccgctt
      241 cttactggcg gcctggccgg tgaagttggg tgcgctgtcg tcgtggggcg ctgcgaatgc
      301 tgagcattct gcccaccctg cgttgtctgg gttcgcgacg gcgcggcggg cgtgccgcta
      361 ttccgattcg ctaccctggg agccggtccc gcacccggtc ggctcgttcc tgccggtcgc
      421 gctgccgtcg cggctgattg ctgtgcgttg ggagcagcga gtcgttgccc tgtcgcaacc
      481 gattgctgcg ctgctggggc agtgggtcgc ggcgctgctg gggcagtggg tcgcggcgct
      541 gttgcggctg attgctgcgc tgctggggcg gtgggtcgcg gtgcggcggg cgcggaactg
      601 gctcgctcag tcgcgcgggg cgggcgagcg gcagttgccg gggtcgtcgc ggttgccttg
      661 ccggttgcgc ttgcggtcgc ttgagacgga gcgcgttggg cagcagccgt tttcgctcct
      721 acttctttgc tcgccgacgt ggtttgtgtg cctggctccg cctttttcgc cgccgcgcga
      781 gggcgagccg gggcggttgt tgcctgccca ttggcatctg gggattcaga tggtttattc
      841 atgaagaaga cccccttcta tttattctct cttcacagcc acataaaaag gactgtctcc
      901 ctttctgcgt cgtgcaggaa ggagacaggc cctcgttggt gcagtgccag gcatgtagag
      961 aagtctgcgc tcactggcgt aggggctatg tcgcttagtt catagctgtc actccgcaaa
     1021 ccgtgccaaa actgccctcc aaacgggggg ctgtgcctct attaagagaa gagatatgct
     1081 gactcatact gttgtaacta ctgctgtctg gttccttgtt ataacagaaa ctgaaaaacg
     1141 cctatccttc gtggatgttt gttttagtat agcacacttc gctcctgcat gtacagcaaa
     1201 tttataccag gtgaatggca tggcgaccgc aagggtcgcc atgctactgt actgtgctaa
     1261 tcctggggca gggttgccac aaaggcatcc aaccctgccc tgtcctcctc ggagatgcct
     1321 acctcgaatt cctgctcaag acgtttcttc ttgagcgcgg tttcccagca cgagagccgg
     1381 gcgcagagat aggcgccgcg cccagatttt ttgccgccgg catccagcag cacatgacca
     1441 tcgggtgtgc gcaccacgcg cagcaactcg cgctttggcc tggtttgctt gcacacaaca
     1501 caggtgcgca tgggtatatg tttggggcgc ttgccctgtt gttttatggc tttcgccatc
     1561 gtgtctgtcc tttgttcgca ctaatcgtct tcttctaccg gtccgctgta tctgccgctg
     1621 ccgcgttgat agggcgcaga acgtttcacc cggtcgcggt cgccgccccg atcatggcgg
     1681 tcgcgccggt cgttacggcc agccgggccg cgccctcctt gatccccgct gcgctcgttg
     1741 cggtcacgac ggccaggata ggcggctgcc ggttcgtgtc gctcatcgag cgtctcttca
     1801 aaggtctcac cctcggcagg cttggtgaca tccacgcgcc acccggtcag cttggcggcc
     1861 aggcgcgcgt tttgcccatc tttgccgatg gccagcgaca actgcctctc ggcgaccgtg
     1921 accagcgcgg tgtggtcgga gtcgcgcaac tgtactttga acggcttcac cgggctgagc
     1981 gcgttcgaga caaaggtcgc ctggtcggga ctccactgga tgatgtcgat cttctcgtcg
     2041 ttcaactcgt gtacgatgtt gttgatgcgc gagccgcgca cacccacaca cgagccgatg
     2101 gggtccagcc cctcctggcg cgccacgact gcaacctttg agcggctgcc gggttcgcgc
     2161 gcgatggctt tgatctcaac cgttccttcg tagatttccg ggacttcctg ctcgaagagg
     2221 cggcgcacca ggtcgcgatg cgtgcgcgag accatgatct ggagcatacg cccctgcacg
     2281 cggcgcacgt cgtaaacgta gacacgcagg cgctggccga tgcgatactt ctcggtcggc
     2341 acctcttcgc ccggcggcat cacaccttcg acccgctctg ccttatcgaa gtcgagcagc
     2401 cagccgcgca cggggtctct gcgtgtcagc acccccagac gaatttcgcc cgtccagtcc
     2461 ttgagttgtt cgtagaggta ttcgtgttcg gcctcgcgga tgcgctgcaa gatgacctgc
     2521 ttggctgtct gcgcgggaat tttgttgaag atatacgacg aatcgacatc aatagtttga
     2581 ccgagcgcgg catgtggaac ctgacgctgc gcttctgcca gagaaatttc gtcgttgggg
     2641 tctgctacct gtgccacgac tcgcctggcg gaccataccc gcacctcgcc attcttgtca
     2701 acctcgatgc gaatattttc tccgtcgcca tagcttttgc gataagccgc caggagcgca
     2761 ttggcaacgg tttccatgac cacttcgcgg ggtaaaccct tctcggcaat caattgtgcg
     2821 atggcagcct gaaattcgct tctcatacgc cttgctcctc cgctagtcgc ctcgttcatg
     2881 gacggtaaca tcattttttc gagtgctatt ccgttacgcc ccgtaacggt tgtcgagtaa
     2941 aaagctttca gtcaagcaga aaagtgggtg agatgcccac ttttctcaaa aggtttcctc
     3001 ggttctgtgc gtattatatc acatctcgcc atgttaagca acgttcattc gacttacatc
     3061 aacaagcgca gaggcgagag aattcgctcg ctcagcttca tataccacga acggttgagc
     3121 catgtgtcga gcgttatttc aaaggcattg ctcagatcgc gctcgaagag ttcctccatc
     3181 tgacacgcca gttgttcgct gtagacctcc gcgttgattt cgtagttgcc gaccgagctg
     3241 aggcggtcca ggttggccgt gccgatggta gaccactggc cgtcgatcga gcaggttttg
     3301 gcgtggagca tgtggcgata accgaagata cgcacgtttg cccgcaagca ttcggtgaag
     3361 ttgccgcgcg ccagccagtc ggtgatgatg tggttggatg tccagggaat cagtacgcgt
     3421 gtatcgacgc cccgctccgc cgcggatttg agcgcgtcga tcagcacgcg gtcggggaca
     3481 aaataggcat cggtgagcag gatgcgttgt tctgctcgat taattgcctc gatatacata
     3541 tcgcgtatgg gaaaggtcag ccgcagcgcg ttcgtctcgt gtatgttgat gagcgggtca
     3601 aaatggcgcg ggtagtggcg tgtgatacgc tgtggcggct tgtgatgcag gttccagaaa
     3661 tccgtgaacg actgcgcaag gtcggcagcc acggaccccc ggatacgcag gtgcgtgtcg
     3721 cgccacatag tggcatacac cttgccgatg ttatagccgc cgataaaggc cgttgcgcca
     3781 tcgaccacca gcagcttgcg atgatccaga gcgtagcggc gcggatcgaa gagatgcagc
     3841 ggcgagccaa tagcgtaata aggcagcgtg tgaatgctgg caggaaagac cttgaactta
     3901 ctcggcacaa ccatattggc gaactggtca aagatcacat agacctcgac accctcggca
     3961 gccttgcgcg ccaggtgttc cttgaatgcc tgcccgacct cgtcgccttt ccagatgtag
     4021 gtttcaaggt agatactctc cttcgcctca tctattgcag caagcatgtc gttgtagaga
     4081 tcgcgtccga agtcgtgcaa ctgcaacacg ttctcgccca ccggcacccg ctccaggtgc
     4141 ccatgcggga atctcccctg gggctgatgc cgtttacgcc ggatcgaaac cacctggaga
     4201 atcaacacga tgctgctttg cagcccgacc atcaggccga atatctgcaa caggagacgc
     4261 cggatcgaga accggcgact ctgacgggca accattgcga gtagtttttt cataggcgat
     4321 acggcaatgc gctcctaatc ccaccgacgt tcatgaaggt ttgatacgac aatcatagca
     4381 catagaagga accagggctg tgatatactc tgaataatat atccgcgttg tacaccctat
     4441 gtaaggagcg aagcatggcg gtttcccatc tgtctgaaga ggagcagggt ttgatcgagg
     4501 ccatacgaga gatcgcgctg gagcgcgtag cgccgcgcgc ggcggagata gaccacacag
     4561 gacaattccc ctgggatatg aaagagttgc tggcgcagca ggatatcctg gcgatgccct
     4621 tcccggtcga ctatggcggc atcggggcga gcgaactggc ggttgttatg gccatcgaag
     4681 aactggcgcg ccactgcgcc actaccgcgc tgatcctggc ggtgcagcag ctcggctcac
     4741 tgccgatcat gctgggcggc aacgagcagc agaaaggcaa gtatctgccg cgcctggcca
     4801 gcggcgaatg gacggcggcc tttggactga cggaggccgg ctccggctcc gacgcggcgg
     4861 ccatgacgac cgtcgcggta cgtaagggcg acaagtatct cctcaacggc tcgaagcgct
     4921 tcatcaccaa tggtgggttg gcgcaggtca actcgatctt cgcgctgacc gataccaccg
     4981 tcgggacgcg cggcatcagc gccttcatcg tcgagcgcga tttccctggc ttctccgtcg
     5041 ggcgcatcga ggacaaaatg ggcattaagg ggtcgcagac ggcagagctg atcttcaccg
     5101 actgcgaggt tccggcggaa aacctgctga gcaaggaggg cgacggcttc aagatcgcta
     5161 tgatgacgct cgaccgcacg cggcccggta ttggcgcgca ggcgctcggc atcgcccagg
     5221 gcgcgctcga cctcgccgtc tcctactcca agcagcgcaa gcagttcggc aagcctatcg
     5281 ccgacaacca gggcatccag ttcatgctgg cggacatggc gacgaaagtt gaggcggcgc
     5341 gcctgctggt ctacaacgtg gcggaaatga tcgcgaatgg cgagcagcac ttcagcaaat
     5401 actcggcaat ggccaagatg tacgcctcgg atatcgccat ggatgtcacc aacgacgcca
     5461 tccagatcct cggcggctac gggtatatga aagaatatcc cgccgaacgc atgatgcgcg
     5521 acgccaagat tacgcagatt tacgagggaa cgaaccagat tcagcgcctg gtgattgccc
     5581 gcgatttgtt gatgtaaggt agagtagaaa tgcgcgaaat caccattttt agcggcaccg
     5641 cccaccacga actggccgac gaggtctgca ggtacctgca tgtccccctt tgcaaaagta
     5701 tcacgcggca ttttggcaac gactgcctgt atgtgcaact gaatgccaac tgccgcgagt
     5761 cggatgtctt cctgatccag ccgcttgtgt cgccggtgca ggaacacttg atggaactgc
     5821 tcttcatgct ggacgcctcg cgcggcgcgt ctgccgcgcg cacgacggcg gttatcccct
     5881 actacgccta cgcgcgctcg gataagaagg acgagccgcg catctccatc gcgggccgcc
     5941 tggtcgccga tttgttgtcc acggcgggcg cgaaccgcgt cctgaccatg accctgcacg
     6001 ctccgcaggt gcatggcttc ttcagcgtgc cgaccgatca tctcaacgcg ctcaaagtgc
     6061 tggccggtca ttttcgcaac cataacctgc gcaacacggt ggtggtttcg cccgacctgg
     6121 gcaacgcgaa atcggcttcg caattcgccc gtttactgcg ggtgccggtc gctgcgggca
     6181 gcaagcagcg cctgagcgac gaccaggtcg tgatcgatac gattgtgggc gatgtcgaag
     6241 gcaaggatgt catcatcctg gatgacgaaa tcgccaatg
//
LOCUS       DASZAK010000237         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_325625, whole genome shotgun sequence.
ACCESSION   DASZAK010000237 DASZAK010000000
VERSION     DASZAK010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325625"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VGT44_03985"
     CDS             complement(<1..184)
                     /locus_tag="VGT44_03985"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2579991.1"
                     /translation="MMFKKILVPLDGSVRAESALPVAARIARASGGSIMLVQVVTIPL
                     MYETTTGGSYSADLIES"
     gene            complement(259..1275)
                     /locus_tag="VGT44_03990"
     CDS             complement(259..1275)
                     /locus_tag="VGT44_03990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2579992.1"
                     /translation="MFKHILVPLDGSTRAESAMPVAARIARAYGSSITLLRVVETIAD
                     HGAYLQPVIEDEKAEAKSYLESIANSTELAGIETSIEIPVGAIAPNIIAAVLTTHANL
                     VVMCSHGYTGIKRWALGSVAEKIARHAPVPVLILREGETFSTLTPQKPVRALVALDGS
                     PFSEAVFEPVAYLVAGLAYSLPQSGELHLLRVIDLPFTSGKLRSQAIIDTQTREQAKQ
                     EAQAYLNGLVARMEEAGLAELNIEATASVESDPDVAEAIVKQAEGSGIGYDLIAMATH
                     GRGGADRFVMGSITERVLHATNLPLLVVRPPKSRTSPVANGKEDVTVIETTDVEVQSW
                     VGLL"
     gene            1664..>2102
                     /locus_tag="VGT44_03995"
     CDS             1664..>2102
                     /locus_tag="VGT44_03995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2579993.1"
                     /translation="MKHVVLANANSRSIGILFMIISAASFGVMPVFARLSYASGADPL
                     TVLFLRFGIAAVVMLGILRLSKTPFPRGLVLIELILLGAIAYVGESLAYFTALTMASA
                     GLVALLLYIYPALVTTLSAIFLKEQLTTPKIVALFLALSGTALT"
BASE COUNT          405 a          633 c          590 g          474 t
ORIGIN      
        1 cactctcgat caaatccgcc gaataggacc cacccgtggt ggtctcatac atcaacggga
       61 tggtgaccac ctgcaccagc atgatcgatc ccccagaggc gcgggcaata cgcgctgcga
      121 ccggcagcgc gctttctgcg cgaaccgacc catcgagtgg aacgagaatt ttcttaaaca
      181 tcatcgttct ccttccagaa cgagaccttt ttctggagag gcgagacgca acgcttgccc
      241 ctccagaaaa caggctctct acagcaatcc cacccaggac tgcacctcta catccgtcgt
      301 ctcgatcacc gtcacgtcct ctttgccatt tgcgacgggc gatgtgcggg acttcggcgg
      361 gcgcaccacc agcagcggca ggttcgtggc gtgcagcaca cgctcggtta tgctgcccat
      421 cacaaagcga tcggctccgc cgcgcccatg tgtggccatg gcgatcaagt catagccaat
      481 cccagacccc tccgcttgct tgacaatcgc ttccgccacg tccgggtcgc tttcgaccga
      541 agcagtcgcc tcaatgttga gttccgctag cccggcttct tccatgcgag ccaccagtcc
      601 gttcaggtag gcttgcgctt cctgctttgc ctgctccctg gtctgggtat cgatgatcgc
      661 ctggcttctc agcttgccgc tggtaaacgg gagatcgatc acacgcagca agtgcagctc
      721 gccggactgg ggcaggctgt aggccagacc cgcgaccagg taggcaaccg gctcaaacac
      781 cgcttctgaa aagggtgagc cgtccagtgc gacgagcgcg cgcaccggtt tctgcggagt
      841 gagcgtggag aaggtctcac cctcgcgcag gatcaagacc ggcaccgggg catggcgcgc
      901 aattttttcg gcaacgctgc ccaaagccca gcgcttgatg cccgtgtagc catgactgca
      961 catgacgacc aggttggcat gggtggtcag gactgccgcg ataatgttcg gcgctattgc
     1021 gccgacgggg atttcgatac tggtttcgat gcctgccagc tcggtcgaat tggcaatact
     1081 ttccaggtaa ctcttcgcct ccgctttttc atcttcaatg accggctgca ggtatgcgcc
     1141 gtgatcggca attgtctcta caacccgcag cagcgtgata gagctgccat atgctcgcgc
     1201 gatgcgcgca gcgaccggca tcgcgctctc cgcgcgggtt gatccatcaa gtggaacgag
     1261 aatgtgctta aacatcgttg tcctcccttc ggcctgcctg agcggagcca gacgcgcccg
     1321 cgtcatgggc aggacgaaca cttaacgact ccttttgact cctttgtcgg acaattccag
     1381 aaagggtggc tgcccggcat ctctctctgt aaagcatagc ttgaaacaat cacaggggta
     1441 tcacatgggg caaagagggt gtcacaaggc tctcacaatg gaaaggacgg tactggaaga
     1501 tgtggcaact atcgcaggcg gtggtgcggt ccgagagcta agtctgtgcg caaaacgtga
     1561 tgtgacgcgc gaatgccgtg tgggatttgt gatactgccg agctatgctt tcttctagag
     1621 gttagtgtat caacctcgct tgagacaaaa agagggcttg tgcatgaaac acgttgtttt
     1681 ggcgaatgcg aattcgcgat ctatcggcat tctattcatg atcatctcgg ctgcttcctt
     1741 tggcgtcatg ccggtctttg cgcggctgtc gtatgcatcg ggggctgatc ccctcaccgt
     1801 cctattcctg cgcttcggca tcgcggctgt ggtcatgctc ggcatcctgc gcttgagcaa
     1861 aacgcccttt ccgcgcgggc tggtgttgat cgagttgatt ttactgggag cgattgccta
     1921 cgttggcgaa tcactggcct attttaccgc gctgacgatg gcctcggctg gcctggtggc
     1981 actgctgctc tacatctatc ccgcgctggt gacgacgctt tcggccattt tcttgaaaga
     2041 gcagctgacg actccgaaaa tcgtcgcgct cttcctcgcg ctttcaggta cggccctcac
     2101 ca
//
LOCUS       DASZAK010000238         1824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_77567, whole genome shotgun sequence.
ACCESSION   DASZAK010000238 DASZAK010000000
VERSION     DASZAK010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77567"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VGT44_04000"
     CDS             complement(<1..785)
                     /locus_tag="VGT44_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV2579994.1"
                     /translation="MQLFNSLSQQIESFVAHNDEVKLYVCGVTPYDTAHLGHAFVFTT
                     FDILVRYLRSRGLRVVYVENVTDIDDPLFERARELGNISWDQLAQQETESFLREMHAI
                     HVVMPDHFVRASEQLPTMFSLIERLLQSGNAYLNDGWIYFDVHSDEQFAQLAFASGLM
                     GYNQLLESANLNGNDPHDPRKRDPLDFLLWRGAVADEPAWPSPWGPGRPGWHIECSAT
                     ATRFLGPQIDIHGGGADLIFPHHSCEIAQTESATGVRPCVRYW"
     gene            complement(883..1821)
                     /locus_tag="VGT44_04005"
     CDS             complement(883..1821)
                     /locus_tag="VGT44_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR domain-containing protein"
                     /protein_id="HEV2579995.1"
                     /translation="MEAGGRLEPDERKRNPRDFALWKAAKPGEPWWESPWGHGRPGWH
                     IECSTMAMETLGEQIDIHGGGADLIFPHHENEIAQSESLSGKPFARYWVHTGLLHIGE
                     EKMAHSGIFVTVRSALEVTPAPALRLYLLSQSYRTPFTYLPEQVGAAVKRWRRWVEAR
                     QAAASFVGDTPAPEREPEEFTHSREEFIAAMDDDFNTSGGMAVVDALVRHLNDITGKP
                     VPGQEREQRELARAGLAVLDELTGILGIALDPALDIQRTIDDATRARIETLVDARTKA
                     RQARNWQEADRIRQELDERFGVIVKDGPTGSTWELK"
BASE COUNT          370 a          572 c          497 g          385 t
ORIGIN      
        1 atccagtacc gcacacacgg gcgtaccccg gtcgcagact ctgtttgcgc gatttcgcag
       61 ctgtgatgcg gaaaaatcaa atctgctccc ccgccatgga tatcgatttg cggccccagg
      121 aaacgtgtcg cggtggccga acattcaatg tgccaacctg gccgtcctgg cccccacggg
      181 ctcggccagg ctggctcatc agcaacagcg cccctccaga ggagaaaatc cagcggatcg
      241 cgctttcgtg ggtcatgggg atcattcccg ttcaggttcg cgctttccag gagctggttg
      301 tagcccatca gcccgctggc aaacgccagt tgcgcaaact gctcatcgct atgcacgtcg
      361 aaataaatcc atccatcatt gagataggca ttgccgcttt gcaacagccg ctcgatgagc
      421 gagaacatgg tcggcaactg ctcggacgca cggacaaagt gatcgggcat gacgacgtga
      481 atggcgtgca tttcgcgcag gaagctctcc gtctcttgct gcgccagttg atcccaggag
      541 atgtttccca actcacgcgc cctttcaaac aagggatcat caatatcggt gacattttcc
      601 acgtacacca cccgcagccc ccgcgaacgc agatagcgga ccaggatgtc aaatgtcgta
      661 aagacgaagg catgcccaag atgggccgta tcatagggcg tcaccccgca cacgtagagt
      721 ttgacttcgt cattatgtgc aacgaagctc tcgatttgct ggcttaagct gttgaatagc
      781 tgcatcgcat tccacctcat ttcttactca ccggacagcg atggggcatg tgaacttgca
      841 gatcattccc tgccatcctg acgagattct agctgtcaag ccctacttga gttcccacgt
      901 cgatcctgtt ggtccatcct tgacgatcac gccgaatcgc tcatccaatt cttggcggat
      961 gcggtcggct tcctgccagt ttctggcttg acgggccttt gtacgggcat caacaagggt
     1021 ctcgatccgg gcacgggtgg cgtcatcaat ggtgcgctga atatccagag ccggatcgag
     1081 agcaatgcca agaataccag tcaactcatc gagcacagcc agcccggcac gtgccagttc
     1141 gcgctgctct cgctcttgcc ctggcactgg tttgccggtg atgtcgttga gatgccgcac
     1201 gagcgcatcg acgactgcca tgccgccgga ggtgttgaag tcatcgtcca tggctgcaat
     1261 aaactcctcg cgagagtggg tgaactcttc gggttccctt tccggcgcag gagtgtcgcc
     1321 cacgaagctg gcagccgcct gccgcgcttc tacccaacga cgccagcgct tgacagccgc
     1381 acctacttgc tcgggaagat aggtgaacgg cgtgcggtat gactgcgaca acaggtacag
     1441 gcgcagggcc ggggccggtg tcacctccaa cgccgacctg accgtcacga agatgccgga
     1501 atgggccatt ttctcctccc ctatgtgcaa cagaccagta tggacccagt agcgtgcaaa
     1561 cggtttgccg ctgaggctct cactctgggc gatctcgttc tcgtggtgcg ggaagatcag
     1621 atcggccccg ccgccatgga tatcgatctg ttcccccagc gtttccatcg ccatcgtcga
     1681 acactcgata tgccaaccag gacgaccatg accccatggg ctctcccacc acggctcccc
     1741 cggtttggct gctttccaga gggcaaagtc acgggggtta cgcttgcgct cgtctggctc
     1801 gagacgccct ccggcctcca tcgc
//
LOCUS       DASZAK010000239         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_289400, whole genome shotgun sequence.
ACCESSION   DASZAK010000239 DASZAK010000000
VERSION     DASZAK010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289400"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VGT44_04010"
     CDS             <1..304
                     /locus_tag="VGT44_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579996.1"
                     /translation="GVERVAREILVVEYFPYFTRRGIGNICLLPSQKYTFHLVREAMR
                     RNAIIIKLRARRQWLGVIPELTSYPNYYELNSPQNTCITQRNCPKGFPEILKALSC"
     gene            523..1377
                     /locus_tag="VGT44_04015"
     CDS             523..1377
                     /locus_tag="VGT44_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2579997.1"
                     /translation="MKSTFLSRLQTSARYALIGALVLVIGVPLYQFLVLTPSGYGNAL
                     MSVGQSLNWIHAHIPLFLGYRALLIVGFSLIVGLPFALFRIIVAQEVLGQDDVSDDDE
                     DLAEEGEDTKDEEEEDEAQTDSEQSEEGADAVANAQEDAMPDFAWRGRGFAVLAAWLG
                     VGGLVLFPLATLASTLYLSLSVPSAASLTGLFAVLTFTVGGGLLALSCLFFGAVITRR
                     GRRLWPDVWLTFGYVALAITLLLSGNAVDVALAPDAGQSVLSTPAILLFALWTLWFGV
                     MVVRLKPE"
     gene            complement(1378..>2381)
                     /locus_tag="VGT44_04020"
     CDS             complement(1378..>2381)
                     /locus_tag="VGT44_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV2579998.1"
                     /translation="SYADTMASYGEQLQQTWSDGEVIDLNQHMIAVTMSIIGKVLFDA
                     DVFTETDELGHAMAVLFEHITHTLTSLFAPPLNWPTPRNRRVQKATQLIQARIVQMIA
                     ERRDTDERDDFLSILLRARHEDGSGMSDEQLMDECITLFGAGHETTAAALTWVWYQLC
                     QHPALYKKVQQEVDSVLQGRTPTYADLPNLPYCLQVFKETMRLYPPATGTMREALRDI
                     EIDGYPVRKGTNVVLPIYTLHRHPRYFPEPETFDPDRFLPEREKALPRYAYLPFGAGS
                     RICIGNHFAMMEGHLLIATLAQRATFSLLPRQMIAPDLTKTLALRPYGKMQVVTKRR"
BASE COUNT          485 a          606 c          700 g          590 t
ORIGIN      
        1 tggggtagaa agagttgccc gtgagatttt agtagttgaa tattttccct attttacaag
       61 aagggggatt ggtaacattt gtcttcttcc ttctcaaaaa tatacctttc atcttgtgag
      121 agaagctatg agaagaaatg ctattattat aaagttgcgc gctagaaggc agtggctagg
      181 ggtgatacct gaattaacga gctatccgaa ctattatgag ttgaacagtc ctcaaaatac
      241 ctgtatcacc caaagaaatt gcccgaaagg ttttcccgag atcctgaaag cactcagttg
      301 ttaagctacg tgtgcggaca cgatcaaagg ggagagtaaa atatttggct cttccgtatt
      361 tgtcatgctc tgctatatag aggaagagcc aaatacttga tagactcctt tgagacagtc
      421 aatcccgttg actttggtta tcaaaggttt acaatggtag gcagaaaacg aagtttctgt
      481 gcgctttaat ccatacattt ctagaggtag cgaagagggg ttatgaaatc gaccttctta
      541 tcacgcttac agaccagcgc gcgttatgcc ctcataggcg cgctggtgct tgtcattggc
      601 gtccctctgt accagttttt ggtcttgacc ccgtcgggct atggaaacgc tctgatgagt
      661 gtcgggcagt cgttgaactg gatacacgcg catatcccgc tcttcctggg ctatcgcgca
      721 ctgctcattg ttggcttcag tttgatcgtc gggctgccgt ttgcgctgtt ccgcatcatt
      781 gtcgcgcaag aagtcctggg gcaggatgat gttagtgacg atgatgaaga tctggcggag
      841 gagggtgagg acacaaagga cgaggaggag gaggacgaag ctcagactga tagtgagcag
      901 agtgaggagg gtgccgacgc ggtggcaaac gcacaggaag atgctatgcc cgactttgcc
      961 tggcgcgggc gtgggtttgc ggtgctggcc gcgtggctgg gcgtcggcgg ccttgttctc
     1021 ttccccctgg cgacgctcgc cagtacgctc tatctctcgc tcagcgtgcc atcagccgcg
     1081 tcgctgacgg ggctattcgc cgtgctgacc ttcaccgttg gcggcggcct gctcgcgctc
     1141 tcgtgtctgt tcttcggggc ggttatcact cgacgaggcc gtcgcctctg gcctgatgta
     1201 tggctgacct ttggctatgt cgccctcgcc ataacgctgc tgctgagcgg caacgcggtg
     1261 gatgtcgcgc ttgcacccga tgccggccag tccgttctca gtacgcccgc tatcctgctt
     1321 ttcgctttgt ggacgctgtg gtttggcgtg atggtggtgc ggctaaagcc ggaataatca
     1381 gcgcctcttc gtcaccacct gcatcttacc atatgggcgc aacgccagcg tctttgtcag
     1441 atcaggcgct atcatctgcc ggggaagaag ggagaacgtg gcgcgttgag caagcgtggc
     1501 gatcaggaga tggccctcca tcatggcgaa atggttgcca atacaaatgc gcgaaccggc
     1561 cccgaatggc agataggcat agcgcggcaa tgctttctct cgttccggca gaaagcggtc
     1621 gggatcaaac gtctcaggct ctgggaagta gcgcgggtgg cgatgcagcg tatagatggg
     1681 cagcaccaca tttgtccctt tgcgtaccgg gtagccgtcg atctcgatgt cgcgcagcgc
     1741 ttctctcatt gttccggtcg cgggcggata gaggcgcatg gtctctttaa agacctgcaa
     1801 acagtacggg aggtttggca ggtcggcata ggtcggtgtg cggccttgca ggacgctatc
     1861 cacttcttgc tgcaccttct tatagagcgc tggatgctgg cagagctggt accagaccca
     1921 ggtaagcgcc gctgccgttg tctcgtggcc cgcgccaaac agtgtaatgc attcatccat
     1981 gagctgttcg tcgctcatcc cgctgccatc ctcgtgcctg gctcgcagca aaatcgacaa
     2041 gaaatcatct cgctcgtcgg tgtcgcggcg ctcagcaatc atttgtacga tgcgagcctg
     2101 gatcaattgt gtcgccttct gcacgcggcg gttgcgcggt gtgggccaat tcaacggggg
     2161 cgcgaagagc gaagtgagag tatgggtaat atgctcgaac agtacggcca tggcgtggcc
     2221 cagttcgtcc gtttctgtga aaacgtccgc gtcaaacagg acttttccaa taatactcat
     2281 cgtcacagcg atcatgtgct gattgaggtc aatcacttcc ccatcgctcc aggtctgctg
     2341 gagttgttcg ccgtaggacg ccatcgtgtc ggcatagctg g
//
LOCUS       DASZAK010000240         8125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_467654, whole genome shotgun sequence.
ACCESSION   DASZAK010000240 DASZAK010000000
VERSION     DASZAK010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_467654"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VGT44_04025"
     CDS             complement(<1..345)
                     /locus_tag="VGT44_04025"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2579999.1"
                     /translation="MATRQVTTDVTRRLDLLRWSRFHTMVTAVLGIGWLLDAFEVNIV
                     GSVLGVIQSVFHLDANQASWVVSIWLLGIMFGAFVFGYLADRFGRKRLFLATLVMYSC
                     FTILSALSPNYIF"
     gene            complement(362..1045)
                     /locus_tag="VGT44_04030"
     CDS             complement(362..1045)
                     /locus_tag="VGT44_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HEV2580000.1"
                     /translation="MRFSIIMPVLNEAAVLEQQLCQLARLCDPYECELLIVDGGSVDA
                     TVAIARRFGRVIAAPRGRAAQMNGGAAVATGDIYIFLHADSMLPDGAFTAIERACAAP
                     GVVGGAFRLCFNCSRLAYRLVAFTTNLRSRWRGIFTGDQAYFVRAESFQAIGGFPDIA
                     LMEDIEIITRLRAIGRLVLLPDYVITSARRHRKVGLARSVLLMWYLRALYRCGVSPEL
                     LQRKYVDVR"
     gene            complement(1057..2487)
                     /locus_tag="VGT44_04035"
     CDS             complement(1057..2487)
                     /locus_tag="VGT44_04035"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2580001.1"
                     /translation="MAEILKQTRSICSTCGEIVPATYEVRDQEQVFFSRRCPTHGIVD
                     TDLGFHAAFYRKSFDVENIMIARYGDGGETDISNGLSPFPLRKPAGLAIVEVTERCNL
                     TCPMCYAYSSPSERDYTLAEIETRLDQLIAIEGKGISLQISGGEPSVRKDLDRIAAMV
                     KSKGFGHLEMVSNGIRLAREPDFAEKLVRWGFTSVYLQFDSTRPEDIVKLRGEDLWDV
                     RVKAIAALERVKLPSTLAVSLYDGLNTDQIQQVVQVAWQHPDTVCAIAFQAATPFGRF
                     EVNNSTGNGQRTTAAPRKLRMPEILQLIEAQTGVSQNLFFPVGEGSPLCNTFTLLKYT
                     REGYKPIAPNFTLKEFMDVIGPRPNMTLRMLTRGRAAVLPQIVSNIGGSLKLMKTLWP
                     HIGTDPSFWTSRKTLTLFVKPFMDESDLDMSRIERCCFHNASPRGVMSFCALNAKMRP
                     ARPHAQESAFVALPTRRVKESVAPAQ"
     gene            complement(2621..3241)
                     /locus_tag="VGT44_04040"
     CDS             complement(2621..3241)
                     /locus_tag="VGT44_04040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2580002.1"
                     /translation="MPSEHDINEMFNERYRTGETPWDTGVSPPELLETMEHPPAELPR
                     RMLDIGCGTGTNCLTLARRGWHTVGVDFAPLAIDAARRKIQGLADEIARAGGSARFLV
                     ADVTRLAPPAPEERYSLLLDLGCLNGIPPAGYANYAKMVAEQALPGALFLLYVHLPVA
                     GRERPRGVTPEELDQLFAAAFRLERREMGSAPQGGQSMWNWLRRVA"
     gene            3470..3844
                     /locus_tag="VGT44_04045"
     CDS             3470..3844
                     /locus_tag="VGT44_04045"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2580003.1"
                     /translation="MIEEERKQQAQFWLDYSHQLAATIRYSEALAAVDRALELDDANA
                     AAWYAKGTMLAMLARYDDALAAFDRALALNEQYAPAWDGKAWALGILGRKHEALAAVN
                     RALELDPEYFDAIKRKKRLEVM"
     gene            complement(3964..4653)
                     /gene="tmk"
                     /locus_tag="VGT44_04050"
     CDS             complement(3964..4653)
                     /gene="tmk"
                     /locus_tag="VGT44_04050"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV2580004.1"
                     /translation="MATGENTLSRENKARRHGKGRLISFEGLDGAGKTTQMELLERWL
                     QAQTISYVRTREPGGTPLGVELRGLLLNRPELAIAPLAEALLFQADRAQHFATLVIPA
                     LDEGKLVITDRCFDASIAYQGYARGVGPELVERLSLLATRGLVPDLTILLDLDPSQVH
                     NRTDATTDTSGLRAERTRFDAEAEAFHTRVQEGFRALARQHPERIKVVDASRSVEEIH
                     REIVGLVRQAL"
     gene            complement(4654..5247)
                     /gene="dcd"
                     /locus_tag="VGT44_04055"
     CDS             complement(4654..5247)
                     /gene="dcd"
                     /locus_tag="VGT44_04055"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEV2580005.1"
                     /translation="MLLSDKRIVEELQHGNIIIEPFDERQLGTNSYDCRLGEWYFQGD
                     MNVDVIHLDNPDDIRRYWGQPRRAEGGKVAIRPGTTILAHTREVIGGHNGYLAKMYSR
                     STVARSGLSVCRCAGVGDVGYISRWTMEISNHTQTTIWAPVGFRICQLTFEYVGETLK
                     EYHGKYGKASHEWTPEDMLPKPYFDWDYDSYRTDRGK"
     gene            5755..7425
                     /locus_tag="VGT44_04060"
     CDS             5755..7425
                     /locus_tag="VGT44_04060"
                     /inference="COORDINATES: protein motif:HMM:NF019445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LecA/PA-IL family lectin"
                     /protein_id="HEV2580006.1"
                     /translation="MKRLLRSKLALSLAAIIMLAAAVLIPFSASITRSYAAQTTPGQC
                     PQGKVCRVTGASTRGTLLPKTNTKNLVVNASQDLPGLDTGIDLTVGTDITITASGQAT
                     FGIGSTQDCNTTEETQPDGQRILSDGTLCPGKIDPSAVLPSSPVGELLASFGSSGWFA
                     VGSSDGFTSAASGRLFLLYNDISGQYGNNSGTYQVVVTTSTPSALTIQGLYTRDGNGN
                     VQNTFAPGATIQYVVNVNNSSGNSLIITIHYQAFGPNQNYIYDQTFPNVTIPQGGSGW
                     YVQGTVPTNAPAGTYTMQVSVVDQNNNNNQDMQTGGQFAVTTANSLWGVDSYSHLTKA
                     FYNSVKATYGTPDFWGRYIGNNPGYPNDMDLTEAPFAHNHGFAILPIYFNYAPNAVNG
                     YSTGQAYATAAIEDAQRRLKISQGVVIFNDIEESSGVNPDAQFIQGWYDQFNSKFSYT
                     YNKKKYTYQAGYYKAGYYGNGTSTSRFASAYCSAVNTEANIGTNSFIWSSEPSGSRTS
                     KTNAPPYGPYTPSCTNQTFAWQYAIEGNGGPPNVDTDEVLSNLPLWHP"
     gene            7746..>8125
                     /locus_tag="VGT44_04065"
     CDS             7746..>8125
                     /locus_tag="VGT44_04065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580007.1"
                     /translation="MKKPAYKWIALAVTTIGSLMVAIDSTIVILALPNMLSDLHSDLV
                     RMTWVIIGYLVVNTVLQLTFGRMADMFGRVKMYNLGFIVFTVGSMLCGLAQSDAMLIG
                     SRLVQGVGGAMLTANAMAIITEVFP"
BASE COUNT         1801 a         2339 c         2225 g         1760 t
ORIGIN      
        1 aaagatgtaa ttcggtgaaa gcgcgctcaa aatagtgaag cacgagtaca tcaccagggt
       61 ggcgagaaag aggcgcttgc gcccaaaacg gtcggcaaga tagccaaaga cgaaagcgcc
      121 gaacataatc cccagcaacc agatactcac gacccacgac gcctggttgg catccaggtg
      181 aaagacgctt tgaataacac caagcacgct cccgacgata ttgacttcaa atgcatcgag
      241 cagccagcca atgccgagga cggcggtgac catcgtgtgg aacctactcc accgcaaaag
      301 atcaagtcgt cgagtgacat cggtcgtgac ctgtcgcgtt gccatcggga atcctttctg
      361 tttaccgcac atccacgtat ttgcgctgca acagttccgg cgagacgccg catcgataca
      421 gggcgcgcag gtaccacatc aaaaggacgc tgcgcgccag gccgaccttc ctgtggcgac
      481 gcgccgaggt gatgacgtag tcggggagca gaaccagcct gccgattgcg cgcaggcgcg
      541 tgatgatctc gatatcttcc atcaacgcta tgtcaggaaa accgccgatg gcctgaaaac
      601 tctcggctcg cacaaaatac gcctggtcgc ccgtgaagat gccgcgccag cgcgaacgca
      661 ggttcgttgt aaacgccacc agccggtagg ccagtcggga acagttaaaa cacagacgaa
      721 acgcgccgcc caccacgccc ggcgcggcgc aggcgcgttc gatagccgtg aaagcgccgt
      781 ccggcagcat cgagtcggcg tgcaggaaaa tatagatatc gccggtcgcc acagccgcgc
      841 ccccattcat ctgcgctgcc cgcccgcgcg gcgcggcaat gacccgtcca aaacgccgcg
      901 cgatagcgac cgtcgcatcc acgctgccgc catcgacaat cagcaattcg cactcatagg
      961 gatcgcacag ccgcgccagc tggcaaagtt gctgttcaag cacggctgct tcgttcagca
     1021 caggcatgat gatcgagaaa cgcatgaggg aatctcctat tgcgccggag ccaccgattc
     1081 tttcacgcgg cgggtgggca gggctacgaa ggcgctttcc tgggcgtgtg gccttgccgg
     1141 gcgcatcttc gcattgaggg cgcagaagga catcacgccg cgcggcgagg cgttgtggaa
     1201 gcagcagcgc tcgatgcggc tcatgtcaag atcgctctcg tccatgaagg gcttgacgaa
     1261 gagcgtcagc gtcttgcggc tcgtccagaa cgacgggtcg gtgccgatat gcggccagag
     1321 cgtcttcatc agcttgaggc tgccgccgat gttggagacg atctgcggca gcacggcagc
     1381 tcgcccgcgt gtcagcatgc gcagcgtcat gttcgggcgc ggcccaatca catccatgaa
     1441 ctctttgagc gtgaagttcg gcgcgatggg tttgtagcct tctctggtgt acttgagcag
     1501 ggtgaaggta ttgcacagcg gcgacccttc gcccaccggg aagaacaggt tttgcgagac
     1561 gccggtctgc gcctcgatca gttggaggat ttcgggcata cgcaatttgc gcggcgctgc
     1621 ggtcgtgcgt tggccgttcc cagtcgagtt gttgacctcg aaacggccaa agggcgtggc
     1681 agcctggaaa gcgatggcgc acaccgtatc gggatgctgc caggcaactt gcacgacctg
     1741 ttgaatctgg tcggtattca agccgtcata gagcgaaacg gccagcgtcg agggcagttt
     1801 aacgcgctcc agcgccgcga tggccttgac gcgcacgtcc cacaaatctt cgccgcgcag
     1861 cttcacaatg tcttcggggc gcgtgctgtc aaattgcagg tagaccgagg tgaaacccca
     1921 cctgacgagt ttctcggcga aatcgggttc gcgcgccagg cggatgccgt tgctcaccat
     1981 ttccaggtgg ccgaagccct tgcttttgac catcgccgcg atgcgatcca ggtctttgcg
     2041 caccgaaggc tcgccgccgg aaatctggag cgagatgccc ttgccctcga tagcgatcag
     2101 ttgatcgagg cgcgtctcga tctccgcgag cgtgtagtcg cgctcggatg ggctggagta
     2161 ggcgtagcac atcggacagg tgagattaca gcgctcggtc acctccacaa tggccaatcc
     2221 tgccggcttg cgcagcggga aaggagacag cccgttggaa atgtcggtct cgccgccatc
     2281 cccatagcgc gcgatcatga tgttttcaac gtcgaacgac ttgcgataga acgccgcgtg
     2341 aaaccccagg tcagtatcga caatcccatg cgtgggacac ctgcgtgaaa aaaaaacctg
     2401 ctcctggtcg cgcacctcgt aagtggccgg gacgatttcg ccgcaggtgg aacagatact
     2461 gcgtgtctgc ttgaggattt cggccaagtt ggtaactcct tttgtacccg gcgccaaaga
     2521 tcgtgaatgc gattcttaaa atggattata ccgccctccc tgctctccct ggaagggcga
     2581 cgcccttttc atctcgcatt cacggcgttt gctcgccgtc tcaggccacg cgccgcagcc
     2641 agttccacat cgattgccca ccttgtggcg cgctccccat ctcgcgccgt tccaggcgga
     2701 acgccgcagc aaatagctgg tcgagttctt ctggcgtaac gccgcggggt cgttctcttc
     2761 cagcgacagg caggtggaca taaagcagga acaatgctcc tggcagggcc tgctccgcta
     2821 ccatcttcgc atagttggca tacccggccg gtggaatacc gttcaagcaa cccaggtcga
     2881 gcagcaagga ataacgttct tcaggagcgg ggggagcaag ccgggtaaca tcggcgacca
     2941 gaaagcgcgc gctgccgccg gctcgcgcga tctcgtctgc gagtccctgt atcttccgcc
     3001 tggcggcgtc aatggcgaga ggagcgaaat cgacaccgac cgtgtgccag ccacgtcgcg
     3061 ccagcgtcag acaattcgta ccggttccgc aaccaatatc cagcatgcgt ctgggaagct
     3121 ccgcgggtgg atgctccatc gtttccagca attcaggcgg gctgacccct gtatcccagg
     3181 gtgtttctcc tgttcgatag cgttcgttaa acatctcatt gatatcatgc tcagagggca
     3241 tattcccatc tcccctttcc tttgcgccac agacgattcg ctccgtgccg cgcgttcgct
     3301 ctcactcgcc acctcgcgtg gttcatacct gtatcatagc ccacaacagg ccgcttgcca
     3361 agagagggaa ggcatcctcc gatgcgcatc attgacaaga ggaacgaaga gattagatac
     3421 ttgaagcagc aggaaatggc agcgaacgat tgtcgatgaa agaacagcta tgatcgaaga
     3481 ggaacgcaaa cagcaggcgc aattctggct ggattacagc caccagttag ccgccacgat
     3541 ccgctacagc gaggcgctcg cggcagtcga ccgcgccctc gagcttgacg acgcgaacgc
     3601 ggcggcatgg tacgcgaagg gaacaatgct ggcgatgctg gcgcgttacg acgacgcgct
     3661 ggccgccttc gaccgcgcgc tcgcgctgaa cgaacaatac gcgcccgcct gggacggcaa
     3721 agcctgggcg ctgggcatcc tcggcaggaa gcacgaggcg ctggcggctg tcaaccgcgc
     3781 attagaactt gacccggagt atttcgacgc gatcaaaagg aagaagcggc tggaagtgat
     3841 gtgagacggt gttttagtta gccagggcga cagcgccgcc aattgtcatc ctgagcgcag
     3901 cgaaggatct cccgccgggc aacgcataga tccttcgctg cgctcaggat gacagctttc
     3961 gcctcacaac gcttgcctaa ccagtcccac aatttcccta tgaatttcct ccacactcct
     4021 tgatgcatcc accaccttga tgcgctcagg atgctgccgc gccaatgcac ggaatccctc
     4081 ttgcacgcgc gtatgaaatg cctctgcctc ggcgtcgaag cgggtgcgct cggcgcgcag
     4141 gccgctcgtg tcggtggttg cgtcggtgcg gttgtggacc tgcgatgggt cgaggtcgag
     4201 caggatggtc aggtcgggga caagacctcg tgtggccagc agggagagcc gctcgaccag
     4261 ctcaggaccg acgccgcgcg cgtagccctg gtaagcgatg ctggcatcaa agcagcgatc
     4321 cgtaatgacc agcttgccct cgtccagggc agggatgacc agcgttgcaa agtgctgagc
     4381 gcgatcggcc tggaagagca gggcttccgc gagtggagcg atagcaagct caggccggtt
     4441 gagcagcagg ccgcgcagtt cgacgccgag cggcgtgcca cccggctcgc gcgtgcgcac
     4501 ataggagatc gtctgcgctt gcagccagcg ttccagcagt tccatctgcg tcgttttgcc
     4561 cgcgccatcc agcccttcaa acgagatcaa gcgcccttta ccatgcctgc gggccttgtt
     4621 ttccctgctc agcgtgttct cgcctgtcgc cacttacttc cccctgtcgg tgcggtagct
     4681 gtcgtagtcc cagtcgaagt agggcttggg cagcatatcc tccggcgtcc actcgtggct
     4741 ggccttgcca tatttaccgt gatactcctt gagtgtttcg ccaacatact cgaaggtcaa
     4801 ctggcaaatg cggaagccga ccggtgccca gatggtggtt tgcgtatggt tgctgatctc
     4861 cattgtccag cggctgatat aacccacatc gccgacgccc gcgcagcggc agaccgagag
     4921 gccagagcgc gccactgtgc tgcgcgaata catcttagcc aggtagccgt tatgcccgcc
     4981 gatcacctcg cgcgtgtgcg ccaggatggt cgtaccggga cgaatggcga ccttgccacc
     5041 ctcggccctg cgcggctggc cccagtagcg ccggatatcg tcggggttgt ccaggtgaat
     5101 gacatccacg ttcatatcgc cctgaaaata ccactcgccc aggcggcaat cgtatgaatt
     5161 ggtgccaagc tgccgctcgt cgaacggctc aataatgata ttgccatgct gcaactcttc
     5221 cacaatgcgt ttatcggata ataacatgct ggcgcgtctc cctcaccttc ctgatgaaat
     5281 agcttcgaag ttattaacgt attatccgcc tgatataggt gatatgcaag aggttggtat
     5341 cacattgtgt gatgatgaaa ggttctccaa cttgaatgaa gaagttaata atacttcctt
     5401 gctactaaat atgaaacacc ttcttatctt actgctagtt cttcttgtgt atttgttctt
     5461 ttgtttcagc aaaagtggtt gagtaatctt aactcatttg atgcaattca tatcacatct
     5521 ctaccaactc gtcactatag aacaatcccc ctccctctac actgaacatg gcgagtaggc
     5581 aattctgctt aaaactcttt tgatgccttc tcaatacggc gacctacggt gggggtatat
     5641 ggtggaggtt cagcgtaaag ccctcatctg gctttttcat ctatggtggc gaccttttct
     5701 ccggcaatta ctggtgcgtt tgtattgcat atttcaattt caggagaacc attcgtgaaa
     5761 cgattactca ggtcaaaatt ggcactgtca cttgctgcaa tcatcatgct cgcggctgct
     5821 gtcctcatcc ctttttcagc ctccatcact cgctcgtatg ctgcacagac cacgcctggg
     5881 cagtgtcctc aagggaaagt ctgtcgcgtg acaggtgctt ccaccagggg aacgttattg
     5941 ccgaaaacaa atactaaaaa cctggttgtc aatgccagcc aggaccttcc agggcttgat
     6001 acgggaattg atttgaccgt aggcactgat attacaatta cggcttcagg ccaggcgaca
     6061 tttggtattg gtagtacaca ggattgcaac actacagaag aaactcaacc cgatggacaa
     6121 cgaattttat cagatggaac gctctgccca ggaaagatag acccatctgc tgtgctccct
     6181 tcgtcgccgg tgggcgagtt gctggctagt ttcggatcat caggctggtt cgccgttggg
     6241 agtagtgacg ggttcacttc ggctgcaagt ggacggttgt ttcttctcta caacgatatt
     6301 tctgggcagt acgggaataa tagcgggacc taccaggtcg tggtcacgac cagtaccccg
     6361 tctgccttga ctatacaggg cctctacacc agggatggta atggcaatgt ccagaatacc
     6421 tttgctcctg gcgctacgat ccaatatgtc gtaaacgtca acaactccag tggtaactcg
     6481 ctgattatca cgatccacta tcaggctttc ggaccaaacc agaactatat ctacgaccaa
     6541 acctttccca atgttaccat tcctcaaggt ggatcgggat ggtacgtaca aggaaccgtg
     6601 ccaacgaacg cacctgccgg gacgtacacc atgcaggttt cggtagtcga tcagaacaat
     6661 aacaacaatc aagacatgca aactggcggc cagtttgcag taacgacagc aaattccctc
     6721 tggggtgttg attcctattc acatctcacg aaggcctttt acaatagcgt taaagcgacc
     6781 tatggtactc ccgacttctg gggacgatat atcggcaata acccaggata tcctaacgac
     6841 atggatttaa ctgaagcgcc attcgcccac aatcacggtt ttgctatcct gccgatctac
     6901 ttcaactacg cccctaatgc agtcaatggg tattcgacgg gtcaagctta tgcaactgcg
     6961 gctatagaag atgcgcaacg gaggctcaag atttcccaag gcgtcgttat cttcaatgac
     7021 attgaggagt cgtccggtgt taatcccgac gcacagttta tacaaggttg gtacgatcaa
     7081 ttcaacagca agttcagtta cacctacaat aagaaaaagt acacgtatca ggcaggctat
     7141 tataaagcag gatactatgg gaacggaaca tccacgagcc gatttgcctc tgcatattgc
     7201 tccgccgtca acacggaagc aaatattggt acgaacagtt tcatctggtc ctctgaacca
     7261 agtggcagtc gaacgagcaa aaccaatgcg cctccatacg ggccttacac accatcttgc
     7321 acgaaccaga catttgcgtg gcagtatgcc attgagggta atggtggacc accaaacgtg
     7381 gataccgacg aagtactttc caatcttccc ctctggcatc catagctgag gatgcactgg
     7441 gttaacagca agcaattgaa cgactaactt aacggccttt ctacaactga aaggccgtta
     7501 agttagtcgt tccgcctggg attagtattg cttttgattt ggctccctac cgggaacgtg
     7561 tgtgatataa tgaacgctat ctaaatcaat aacaggagaa ggcacacctt ctcctgttcg
     7621 cttttgtaat atcgtccagc taaacaggct gaattgcgtc cagagccatc atcagctttg
     7681 tgtctcatgc ctttcgtttt ttccggcgcc ttctaccagg gtttcgcgct agagagaagt
     7741 cttcaatgaa aaagcccgcc tacaagtgga tcgcgcttgc cgtgacaacc atcggctcgt
     7801 tgatggtcgc tatcgactca actatcgtca ttttagccct gccgaatatg ctctccgacc
     7861 tgcattccga tctggtgcgt atgacctggg tcatcatcgg ctacctggtc gtcaataccg
     7921 tcttgcagct cacatttggc cgcatggccg acatgttcgg acgggtgaag atgtacaacc
     7981 tgggctttat cgtctttacg gttggctcga tgctgtgcgg gttagcgcag agcgatgcca
     8041 tgctgatcgg ttcgcgcctg gtgcagggcg ttggcggggc gatgctgacg gcgaacgcca
     8101 tggccatcat caccgaggta ttccc
//
LOCUS       DASZAK010000241         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_432526, whole genome shotgun sequence.
ACCESSION   DASZAK010000241 DASZAK010000000
VERSION     DASZAK010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432526"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VGT44_04070"
     CDS             <1..712
                     /locus_tag="VGT44_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580008.1"
                     /translation="MWMQMIQSQQLVYSLLARPAILFLIVAAISYLWVNSALNALKRA
                     DRAELIAELERRELEQKQELERAIEHILLVQTRVANGDFDARVPIYEHHVLWQVSIAF
                     NNLLARFKSSAQSERNLHRVAEEIAQLRSALKLWQLGQPLRWYPTEKNLLGPLADDLR
                     RTMLPQVAASAAPQGVQAAAQMPHANQGDIRYRSPMTDPGEPAQHIRYRSPVTEPGEP
                     VEQASPPHAYQRPWPRMA"
     gene            795..>1633
                     /locus_tag="VGT44_04075"
     CDS             795..>1633
                     /locus_tag="VGT44_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HEV2580009.1"
                     /translation="MFKAHRKRLLGYALMAFSILFLLTAWPASTSPSTRTPSTPTPLD
                     SSSSSLRSLARQRDFFIGSAVNYDALENEPLYRQALAREFNLVTAEDSMKFDHTEQQP
                     GVFTFGEGDTILAFARAHGMTVRGHNLVWYRALPDWIAKGTFTRDQLIAILRDHIMTE
                     VSHYRGTVNIWDVVNEAIDDNGNLRDSIWLRVIGPDYIDMAFRWAHEANPQALLFYND
                     YGGEGLNHKSDAIYALVKGLVSRGVPINGVGLQMHVSLDRYPSPHDVLANMQRLAALG
                     LQV"
BASE COUNT          318 a          531 c          449 g          335 t
ORIGIN      
        1 gatgtggatg cagatgattc aatcgcagca gcttgtctat tcgctgctgg cccgtcctgc
       61 cattctcttt ttgattgtcg cggccatttc ctatctctgg gtcaatagcg ccttgaatgc
      121 tctgaagcgc gcggaccggg ctgaactgat cgccgaactt gagcggcggg aactggaaca
      181 gaaacaggaa ctcgagcggg ctatcgagca tatcctgctg gtccagacgc gcgttgccaa
      241 cggcgatttc gacgcgcgcg ttcctatcta cgaacaccac gtcctctggc aggtcagtat
      301 cgccttcaat aacctgctgg cgcgcttcaa atcatccgcg caaagcgaac gcaacctgca
      361 tcgtgtcgcg gaagaaatag cgcagctgcg ttccgccctg aagctctggc aactcggcca
      421 acccctgcgc tggtacccga ccgagaagaa cctgctcggc ccgctcgccg acgacctcag
      481 acgcaccatg ctgccccagg tcgctgccag cgcagcacct cagggtgttc aggctgctgc
      541 tcagatgccc catgcaaacc agggggatat ccgctaccgc agcccaatga ctgaccctgg
      601 cgagccagcg caacatattc gctatcgcag cccagtgact gagcctggcg agccagtaga
      661 gcaagcaagc ccgccccacg cttaccagcg tccctggccg cgcatggcgt agcccatttg
      721 cttcgcaaat tgacacagtc agccgctttc tgctactgta cgcgcagtag taaacgcgaa
      781 tgaggtacac gttagtgttc aaagcgcatc ggaagcggct gctgggctat gccctgatgg
      841 ccttctccat cctcttcctt ttgacggcgt ggcccgcttc cacgtcgccc agcacacgca
      901 ctccatcgac gcctactccc ctggattcct catcgtcttc gctgcgttcg cttgcccggc
      961 agcgcgactt tttcatcggc tcggcggtga actacgatgc cctggaaaac gagccgcttt
     1021 accgccaggc gctcgcgcgc gagttcaact tggttacagc agaagactcc atgaagtttg
     1081 accatactga gcagcagccg ggcgtcttta ccttcgggga gggcgatacc attctggcct
     1141 tcgcgcgagc gcacggcatg acggtgcgcg gccacaacct ggtctggtat cgcgccctgc
     1201 cagactggat cgcgaagggc accttcacgc gcgatcaact catcgccatc ctgcgcgatc
     1261 atatcatgac cgaggtcagc cactatcgcg ggacggtcaa catctgggat gtcgtgaacg
     1321 aggctatcga cgacaacggc aacctgcgcg attcgatctg gctgcgcgtc atcggccccg
     1381 attacatcga tatggccttt cgctgggcgc acgaggccaa tccacaggcg ctgctctttt
     1441 ataacgatta tggcggcgag gggctgaacc acaagtcgga tgccatctat gccctcgtta
     1501 agggccttgt ttcgcgcggc gtcccgatca acggcgttgg cctgcaaatg catgtctctc
     1561 tcgaccgata ccccagcccg catgatgtgc tggccaatat gcagcggttg gccgcgctcg
     1621 gtctgcaagt gca
//
LOCUS       DASZAK010000242         2829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_574142, whole genome shotgun sequence.
ACCESSION   DASZAK010000242 DASZAK010000000
VERSION     DASZAK010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574142"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1815
                     /locus_tag="VGT44_04080"
     CDS             343..1815
                     /locus_tag="VGT44_04080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580010.1"
                     /translation="MNKPAYKWIALAVTTIGSLMVAIDSTIVILALPNMLSDLHSDLV
                     RMTWVIIGYLVVNTVLQLTFGRMADMFGRVKMYNLGFIVFTVGSMLCGLAQSDAMLIG
                     SRLVQGVGGAMLTANAMAIITEVFPAEQRGQAMGINAITWGAGSVLGPVLGGFILAFA
                     SWRWIFLVNLPIGILGTLAAYLLLHDIAPNPRGERFDLLGAVLFCIGLVCLLLGMTGG
                     IGSGWLSPSILALLGVGVLAFILFIIRERRVAYPMLDLHLFDNRQYGLSVAAATLQSL
                     ATFAVNFILIFYLQGVRGYTPLTAALLILPLPVMTSVFGPLGGRWADRVGGAVPATIG
                     LVIQIAALIVLVLLTPATPYLVLALALALMGIGGALFWSPNTSTAMGAAPRNRLGVAS
                     ATLNTLRNVGMVFSFALALSVAAASMPPVLVSEIFLGTVVHLQASIATAFTGAMSHAF
                     IASILICVAALGFSAIRENKLRLRAAEKTTQANPGGSAGQ"
     gene            complement(1820..2083)
                     /locus_tag="VGT44_04085"
     CDS             complement(1820..2083)
                     /locus_tag="VGT44_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HEV2580011.1"
                     /translation="MAFVITHPCIGSKDASCVDVCPVDCIHPTPDERWFAQVDHLYIN
                     PGECIDCGACQPACPSNAIYHESAVPREWKHYIRANADFYQRR"
     gene            complement(2198..>2829)
                     /locus_tag="VGT44_04090"
     CDS             complement(2198..>2829)
                     /locus_tag="VGT44_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEV2580012.1"
                     /translation="LHLDRCPGPCRGGADPDAYRKTIDEVCAFLGGEREDLLDRLRRQ
                     MLEASQNLNFERAAWLRDTIRSADEVLIGQQLITGAVESNNLFIIYPSAREGCNELFL
                     VRHGRLVHQRCIPHEPETTKTAVHELLVLAVTMGEPPSIVGKAEVDQINIISRWIHHH
                     SNDRAFFPLQPALADADEARSLAQRVWVEIEAMRALPVAQAATGDIADL"
BASE COUNT          490 a          856 c          848 g          635 t
ORIGIN      
        1 tgaaaggccg ttaagttagt cgtcccgcct gtggattagt attactattg atttggctag
       61 gtgcgcgcta ttttttgtac acagggcaag cgttccagag ggcgcaccct tgtggtcgcc
      121 ctctggaacc tcatttggct ccctaccggg aacgtgtgtg atataataag tgttatctaa
      181 atcaataaca ggagaaggca caccttctcc tgttcgcttt tgttatgtcg tccagctaaa
      241 caggctgaat tgcgtccaga gccatcacca gctccgtgtc tcatgccttt cgtttttttc
      301 cggcaccttc taccagggtt tcgcgctaga gagaagtctt caatgaataa gcccgcctac
      361 aagtggatcg cgcttgccgt gaccactatc ggctcgttga tggtcgccat cgactcaacc
      421 atcgtcattt tggcactgcc gaatatgctc tccgacctgc attccgacct ggtgcgtatg
      481 acctgggtga ttatcggcta cctggtcgtc aataccgtct tgcagctcac atttggccgc
      541 atggccgata tgttcgggcg agtgaagatg tacaacctgg gctttatcgt ctttacggtt
      601 ggctcgatgc tgtgcgggtt agctcagagc gatgccatgc tgatcggttc gcgcctggtg
      661 cagggcgttg gcggggcgat gctgacggcg aacgccatgg ccatcatcac cgaggtattc
      721 cctgccgagc agcgcgggca ggccatgggc atcaacgcta tcacctgggg cgcgggcagc
      781 gtgcttggcc cggtgctggg cggcttcatt ctagccttcg cgagctggcg ctggattttc
      841 ctggtgaacc tgcccatcgg cattctcggc acgctggcgg cctacctgct gctgcacgat
      901 atcgcgccga acccgcgcgg cgagcgcttt gacctgctcg gcgcagtcct cttctgtatc
      961 gggctggtct gcctgctgct cggcatgacg ggcggaatcg gctccggctg gctctcgccc
     1021 tcgatcctgg ctctgctcgg cgttggcgtg ctggccttca tactcttcat cattcgcgag
     1081 cggcgtgtag cctatccaat gcttgatctg catctctttg ataaccggca atacggcctc
     1141 tctgtcgcgg cggccactct gcaatcgttg gcaaccttcg ccgtcaactt catcctcatc
     1201 ttctacctgc aaggtgtgcg tggctatacg cccttaaccg ccgcgctgct gatcctgccc
     1261 ctgccggtca tgacctcggt cttcggcccc ctgggcgggc gctgggcaga ccgcgtcgga
     1321 ggcgcggtac ccgctacaat cggcctggtg atccagattg ccgcgctgat cgtcctcgtg
     1381 ctgctcaccc ccgcgacgcc ctatctcgtg ctggcgctcg cgctggccct gatgggaatc
     1441 ggcggcgcgc tgttctggtc gcccaatacc agcactgcca tgggggccgc gccgcgcaac
     1501 cgcctgggcg tcgcctcggc cacgctcaac acgctgcgta atgtgggtat ggtgttcagc
     1561 ttcgcgctcg ccctgtcggt cgcggcggcc tcgatgccgc ccgtgctggt ctcggaaatc
     1621 ttcctgggaa cggttgtcca cctgcaagcc agcatcgcca ctgccttcac cggtgcgatg
     1681 agtcacgcct tcatcgcctc gatcctgatc tgcgttgccg cgctgggctt ctccgccatt
     1741 cgcgaaaaca agctcaggct gcgcgccgcc gaaaagacga cgcaggccaa ccccggcggc
     1801 agcgccgggc aatgaacgtt tagcgacgct ggtagaaatc ggcattggct cggatatagt
     1861 gtttccattc tctgggaacc gccgactcgt gatagatggc gttggatgga caggccggtt
     1921 gacacgcgcc acagtcaatg cactcgccag gattgatata aaggtgatcc acctgcgcga
     1981 accagcgttc gtccggtgtc gggtgaatgc agtctaccgg gcaaacatct acgcaagacg
     2041 catctttcga tccaatgcag gggtgagtaa ttacaaaagc caaaacgtat ctcctctttc
     2101 attattgttt atacatatat aagcccacga tagcgcacag gtatcaaaaa agtggactgg
     2161 attgtgtaaa gagtctgtaa aaaaagcgcg acagttccta cagatcggcg atatcgcccg
     2221 tcgcagcttg cgccaccggc agggcgcgca tggcctcgat ctccacccag acgcgctggg
     2281 ccaggctgcg ggcttcgtcg gcgtcggcga gggcgggctg cagggggaag aaggcgcggt
     2341 cgttgctgtg gtgatgtatc cagcggctga tgatgttgat ttgatcgacc tcggccttgc
     2401 cgacgatgct gggcggttcg cccatcgtta cagccaggac gagcagctca tgtaccgccg
     2461 ttttggtcgt ctccggctcg tgggggatgc agcgctgatg caccaggcgg ccatgccgca
     2521 cgaggaagag ttcgttgcag ccctcgcgcg ccgaggggta aatgatgaag aggttgttgg
     2581 actcgaccgc gccggtgatg agctgctggc cgatcaagac ctcgtcagcg ctgcgaatag
     2641 tgtcgcgcag ccaggccgct cgctcgaaat tgaggttctg cgaggcttca agcatctggc
     2701 ggcgcaggcg atccaacaaa tcctcgcgct cgccgcccag gaaagcgcac acctcgtcga
     2761 tcgttttgcg gtatgcgtcg gggtccgcgc cgccgcgaca agggccgggg cagcggtcga
     2821 ggtgaagcc
//
LOCUS       DASZAK010000243         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_325888, whole genome shotgun sequence.
ACCESSION   DASZAK010000243 DASZAK010000000
VERSION     DASZAK010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325888"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VGT44_04095"
     CDS             <1..724
                     /locus_tag="VGT44_04095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2580013.1"
                     /translation="GASRGLGRAIVAAAHAEGAQVLAVARKVAELAKLTEELAGVQVL
                     ALDATSLSAPAQVFATLSPDVLLVCAGAVPHMAPLGEQSWEQFSRNWNTDVKISLFFL
                     QAALNTPLPEGSTIILMSSGAAIGGSPLSGGYSGAKREQMILASYAQEESDRLHLGLR
                     FLALAPGNIMPETDMGKTAASAYAASRGLSVEDFASLQTGGQPLVTPDLVANAVIELA
                     SDPQKSAGKSFLLSGKGMEIVP"
     gene            904..2340
                     /locus_tag="VGT44_04100"
     CDS             904..2340
                     /locus_tag="VGT44_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580014.1"
                     /translation="MRAWVLVLTSLAACMISLDSQVVATALSTIRLHLGASIEELEWT
                     VNAYTLSFAELLMTGAALGDRFGRRRLFVAGLGLFVLASAACALAPNAGWLIAARALQ
                     GCGSALVMPLALTLLSTAFPPERRGRALGIFTSVAGLALLVGPVVGGAIAQGLAWQWI
                     FWLNVPIGLIVIPLVLGRIQESFGPRTVLDIAGLVLITGAIFGLVWGLVRGNSAGWGS
                     LEVVASLVAGVGLAVAFVVWELRARVPMVPMRFFRSRAFSAGNTANFLLFAAIFGTLF
                     FLAQFLQTAQGYGPLTAGLHLLPWTACLFVVAPISGSLVNRVGERPLIVGGLLLQAIG
                     LAWIGLIARPDLAYAGFVVPLIIAGIGGSLAMPAAQNVVVSAVAPNEIGKASGIFSML
                     RQLGGAFGVAILAAVFASIGGFRSAQAFSNGFSPAMSVAAALSLMGAIAGLVLPGRRA
                     RTVVQTQAKAPEIRENELSGAPEQSSIL"
BASE COUNT          452 a          754 c          822 g          556 t
ORIGIN      
        1 cggagcgagc cgtggtctgg gacgcgccat tgtggctgcg gctcatgcag aaggcgcgca
       61 ggtgctggcc gtggcgcgaa aggtcgcgga gcttgccaag ctcactgagg aactcgcagg
      121 cgttcaggtg ctcgcgcttg acgccaccag tctaagcgct cccgcccagg tgtttgcgac
      181 cctttcgcct gatgtgttac tcgtctgcgc aggggcggtc cctcatatgg ctccgcttgg
      241 agagcagagt tgggaacaat tctcgcgcaa ctggaatacc gatgtgaaga tatcgctgtt
      301 cttcttgcaa gcggcgctga acacgccact gcccgaaggc tccacgatca tcctgatgtc
      361 cagcggggca gccatcggcg gttcgccgct ttcggggggg tattctggcg ccaagcgcga
      421 gcagatgatt cttgccagtt atgcccaaga agaatctgat cgcttgcatc tcggtttacg
      481 cttcctggcg ctggcaccgg gcaatattat gccagaaacg gacatgggga agactgcggc
      541 ctctgcttac gctgcttctc gaggtctttc cgtcgaggac ttcgcctccc tccagacggg
      601 cgggcagcct ctggtcacgc cagacctggt tgccaacgcc gtgatagagc ttgccagcga
      661 tccgcaaaag agcgcaggaa agtcgttcct gctttctgga aaagggatgg agatcgtgcc
      721 ctagtcctac aggtggagat aacactgctg gcccgaaaca gaggatgggg agatgagaga
      781 ggccctttct gaatgtacag ccatgcaccc cttttctcaa gatgctcctg tatccacagg
      841 agtgcgaaac ctttgtttgg agtgaagaag aagaacatga tacaaaaagg aagcgaaaaa
      901 gcaatgaggg cctgggtcct tgtgctcacg tcgctggcag catgtatgat ctcgctcgat
      961 tcgcaggtcg tcgcgactgc cttgagtacg atccgtctgc atctgggtgc ctccatcgag
     1021 gagttggagt ggacggtaaa cgcctacacc ttgagcttcg cggagctgct tatgactggc
     1081 gcggcgctcg gcgaccgttt cgggcgtcgg cgcctgttcg tcgctggcct ggggctgttc
     1141 gtactggcgt cggcggcctg cgcactcgcg ccgaatgctg gctggctgat cgccgcacgc
     1201 gctctgcagg ggtgcgggtc ggcactggtg atgccgctgg cgctgacgct gttgagcacg
     1261 gcattccctc ctgagcggcg gggcagggcc ctggggatct tcacgagcgt ggccgggctg
     1321 gccctgctcg tcggcccagt ggtgggcggc gccatcgctc agggcctcgc ctggcagtgg
     1381 atcttctggc tcaacgtccc gatcggactc atcgtcattc cgctcgtact tggtcgtatc
     1441 caggagagtt tcgggccgcg cacggttctc gacatcgctg gtctggtgct gataacgggg
     1501 gcgatcttcg ggctggtgtg ggggctggtg cgcgggaaca gcgctggctg gggcagtctt
     1561 gaggtcgtcg catccctggt ggccggggtt gggcttgcag tggcctttgt ggtctgggaa
     1621 ctgcgcgctc gcgtgccgat ggtgccgatg cgcttcttcc gctcccgcgc cttctcagcg
     1681 ggcaacacgg cgaacttctt gctcttcgcg gcgatattcg gcaccctgtt ctttttagcg
     1741 cagtttttgc aaaccgcgca gggttacgga ccactgactg ccgggctgca cctgcttccc
     1801 tggaccgcct gcttgttcgt ggtcgccccg atctccggct ccctcgtcaa ccgggtcggc
     1861 gagcggccac tcatcgtcgg cgggctgctg ctgcaagcca tcgggctggc atggatcggg
     1921 ctgatcgcca ggcccgacct cgcctatgct gggtttgtcg taccgctgat catcgctggc
     1981 ataggcggct cgctggccat gccagccgcc cagaacgtcg ttgtcagcgc ggtggcaccc
     2041 aacgagattg gcaaagcatc cggcatcttc agcatgctgc gccagctcgg tggggcattt
     2101 ggggttgcga tcctcgccgc cgtgtttgcc agcatcggag gctttcgctc cgcccaggcg
     2161 ttcagcaacg ggttttcccc ggcgatgagc gtcgcggcgg ctctgtcact gatgggagcc
     2221 atcgctggcc tggtactacc cggcaggcgc gcaagaactg tcgtacaaac gcaggcaaag
     2281 gcgcctgaga taagggaaaa cgaactctcc ggcgcgccgg agcaatcctc gatcttgtaa
     2341 atcccagaaa gagtagcttg agctgtagaa gtcgtataga ctaaggaatg attgcatcac
     2401 gattcaagcc tgcaacggaa ctgacgctcc attgcaggct tgagcaacga ttctacatct
     2461 atggagagag ggtagatgtt acacgtccct cctaggattc atcatgggga gtttcctctt
     2521 caaccgcact atgcccttcc tcaacaacaa aagaagtgga tcccacaaga tgatgaaccc
     2581 gata
//
LOCUS       DASZAK010000244         1950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_610221, whole genome shotgun sequence.
ACCESSION   DASZAK010000244 DASZAK010000000
VERSION     DASZAK010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1950
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610221"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VGT44_04105"
     CDS             <1..475
                     /locus_tag="VGT44_04105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEV2580015.1"
                     /translation="NGSVTTLTVYAFGLEDHVYSGAGTNQGNTYYYSLAGHLIGALDS
                     SGTTFYLTDALGSILTSFTNASGSAAVKGEQDYAPYGPSRYTQGTINTPKGYTGQYND
                     SLTGLDYYGARYYDPVVGVFLSADSVQGNPQGMNPYGYEPYTYIPQRSPANQSSR"
     gene            621..1775
                     /locus_tag="VGT44_04110"
     CDS             621..1775
                     /locus_tag="VGT44_04110"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEV2580016.1"
                     /translation="MDLQTLVREVEDYTQQHLSTYIEELRALCAIDSDSYNKAGLDAM
                     AAWLGERMRGLGMDVTIVERAEWGNDLLGVLRGTGQGNVLLLGHIDTVYPVGTAAERP
                     LRVEGETAYGPGVCDMKGNVLAAIYAIEALQVSDYGEIRFLCVSDEEINTRHCHDVMQ
                     QVCQDCQRALVLEAARANGDIVSARKGHTWYTLTAQGRSAHAGVEPEKGRNAIVEIAH
                     QVLQFEDLNGWREGITVNAGTIAGGTVANVVPDFAKAVFDLRFLSYDDRKATERRFHE
                     LMEQKRVPDVALTLEVAPDIKGPMVRTPASLRLAEQAQHLAQLLGFSVNHVLTGGASD
                     ASFTSEAGVPTLDGLGPIGGRDHSPYEYLMLDSVAPRAALLAGLIVTTAS"
BASE COUNT          406 a          548 c          596 g          400 t
ORIGIN      
        1 caacggcagc gtcaccacgc tcaccgtcta cgcctttggc ctggaagacc acgtctacag
       61 cggcgctggc accaaccagg gcaacaccta ctactacagt ctggccggac atctaatcgg
      121 cgcgctggat agcagcggca ctaccttcta cctgactgac gcactgggca gcatcctcac
      181 cagcttcacc aatgcctccg gctcggccgc cgtcaagggc gagcaggact acgcgcccta
      241 cggcccgtcg cgctacaccc agggcacgat caacacgccc aagggctata ccggccagta
      301 caacgactca ctcacggggt tggactacta tggagcgcgg tactacgacc cggtggtggg
      361 cgtgttcctc tcggcggata gcgtccaagg caatccacag ggcatgaacc cctatggcta
      421 tgagccttac acatatattc cacaacgttc ccctgcaaat caatcaagta gataaaggtg
      481 agaagagcta cgctgacatt acaaaattga cctggttaga tctctatcga gagtgataaa
      541 gtttgttcct atcgctcctc attcccctgt tgctcaccta atcccatctc cgtctaatac
      601 tatggaggta atttattctt atggacttac aaacactggt tcgcgaagta gaagactata
      661 cacaacaaca tctctccacg tatatcgagg agttgcgcgc gttgtgcgct atcgactcgg
      721 atagctataa caaggcgggg ttggatgcga tggcggcatg gctgggggag cggatgcgcg
      781 ggttggggat ggatgtgacg attgtcgagc gtgccgaatg gggcaatgat ctgctggggg
      841 tgctgcgcgg cacggggcag ggcaatgtgc tgctgcttgg gcatatcgat acggtttatc
      901 cggttggcac tgctgctgag cgcccgctgc gcgtggaggg ggagacggcc tatggcccag
      961 gagtgtgtga tatgaagggc aacgtgctgg cggcgatcta tgctatcgag gcgctgcagg
     1021 tgagcgacta tggcgaaata cgttttctgt gcgtctcgga tgaagagatc aatacgcgcc
     1081 actgccatga tgtgatgcag caggtgtgcc aggactgcca gcgcgcgctc gtgctggagg
     1141 ccgcgcgggc caatggcgat atcgtcagcg cgcgcaaggg gcatacctgg tatacgctga
     1201 cggcgcaggg gcgttctgca catgccgggg tcgagccgga gaaggggcgt aacgccatcg
     1261 tggaaatcgc gcaccaggtt ctgcaattcg aggacctcaa cggttggcgc gagggtatca
     1321 ccgtgaatgc cgggaccatt gctggcggca cggttgccaa tgttgttccc gacttcgcga
     1381 aggcggtgtt cgacctgcgc ttcctcagct atgatgatcg caaggcgacg gagcggcgtt
     1441 tccacgagtt gatggagcag aagcgcgttc cggacgtggc gttgacgctg gaggtcgcgc
     1501 ccgacatcaa aggtccgatg gtgcgcacac cggccagcct ccggctggcg gagcaggcgc
     1561 agcatctcgc gcaactgctc ggcttttccg tcaatcatgt cctgaccggc ggtgcgtccg
     1621 acgcgagctt tacctcggag gccggtgttc cgacgctgga tggcctggga ccgattgggg
     1681 ggcgcgatca cagcccctac gaatatttga tgctcgatag cgtcgctcct cgtgccgccc
     1741 tgctcgccgg gctgatcgta acgaccgcgt cgtaattgta aaacgtgcaa acacgcccat
     1801 ccgtaggtgc cgagtatgaa tttttacaac aaaaatcagg aatgtcatgc tgagcgcagc
     1861 gaagcatccc tatgtccatc cagtgagacc agacccttcg cttcgctcag ggtgacaatc
     1921 gatggccgat cctggttgtg aaagttcatt
//
LOCUS       DASZAK010000245         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_680943, whole genome shotgun sequence.
ACCESSION   DASZAK010000245 DASZAK010000000
VERSION     DASZAK010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680943"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VGT44_04115"
     CDS             complement(<1..1139)
                     /locus_tag="VGT44_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2580017.1"
                     /translation="MKCERCGQNPAQVRIDQIINGKREQHYLCKSCVDELMSDAMNQM
                     GGLDSSGLPDGAPFGFVGKNNDNSDNPVFSRSGRNTATAQQQAKHSKTPTLDQYGRDL
                     TAEAAAGKLDPAAGRARELRRLITVLGRRQKNNPVLIGEPGVGKTAIVEGLARRINDG
                     DVPANLRNRRIVTLNIGGMVAGAMFRGQFEQRIKSILEELRQSEDVIVFIDELHTVVG
                     AGAAEGAVGASDMLKPALARGELRCIGATTLDEYRKHIEKDAALERRFQPVMVDEPSE
                     EEAIEMLKIVRANYELHHGIGITDEAVDAAVKLSNRYINDRFLPDKAIDVMDEAGSAL
                     RLEATEKGIISPNTISDLEKEISAIQAQKEAAANAEDYERAAKLR"
     gene            1623..2009
                     /locus_tag="VGT44_04120"
     CDS             1623..2009
                     /locus_tag="VGT44_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580018.1"
                     /translation="MPYDSSSAGGILAAVGGVTLIVFLAIAVFTVIIYWRIVSKAGYN
                     GAMSLLLFIPIANLIMIIIFAFSEWPIQKELNQLRAMSGRGGPGGPGSPFPSSPQYPG
                     YGQPQYGQPGQPQYGQPGQPPQYPQR"
     gene            2275..3483
                     /locus_tag="VGT44_04125"
     CDS             2275..3483
                     /locus_tag="VGT44_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2580019.1"
                     /translation="MDFAYTEKVNQLRKRVSDFMERYIYPNQSTYRQQIEASGDPHHH
                     AEITDELKAIARSEGLWNLFMPDDEYGAGLTNLEYAPLAEIMGRVSWSSEVFNCAAPD
                     TGNMEILAQFGTPEQKRAWLMPLLNGEIRSAFAMTEPEVASSDATNIQLTIRREGDEY
                     VLNGHKWWISGAARARCKIFIVMGKTAPDAPDTHRQQSMVLVPKDTPGLTIVRNLPVM
                     GYIDNESHCEMLFENVRVPVDNLLGEEGGGFAIAQARLGPGRIHHCMRAIGGAQYALE
                     LMCKRAASRVAFGKPLLDQGVIQEWIAQSELEIEQARLLTLKAAWMIDTLNKKAAQKE
                     IAMIKIAAPEVFTNVVSRAMQVFGAAGLSDDFPLASMWAHGRTLHIADGPDEVHKRAV
                     ARIVIRQLAR"
BASE COUNT          761 a         1096 c          992 g          744 t
ORIGIN      
        1 tggcgcagct tcgcagcgcg ttcgtagtcc tcggcattgg ctgccgcttc tttctgcgcc
       61 tggatcgctg agatttcctt ctccaggtcg gagatggtgt tcggactgat gattcccttt
      121 tcggtcgcct caagacgcag agccgagcca gcctcgtcca tcacatcgat agctttgtcc
      181 ggcaggaagc ggtcgttgat atagcggttg gagagcttga cggcggcatc gacggcctcg
      241 tcggtgatgc cgatgccatg atgcagctcg tagttggcgc gcacgatttt gagcatctcg
      301 attgcctctt cttcggatgg ctcatcgacc atcacgggct ggaagcggcg ctccagagcg
      361 gcatccttct caatgtgctt gcggtactcg tcgagcgtgg tcgcgccaat gcagcgcaat
      421 tcgccacgcg ccagcgccgg cttcagcata tcggacgcgc caaccgcgcc ctcagccgcg
      481 cccgcaccca cgacagtatg caactcgtcg atgaagacga tcacgtcctc ggactgtcgc
      541 aattcttcga gaatggactt gatgcgctgc tcgaactggc ctcggaacat cgccccggcc
      601 accatgccgc cgatgttcag cgtgacgata cgcctgttgc gcaggttggc cggcacgtcg
      661 ccatcgttga tgcgcctcgc caatccttcg acgatggcgg ttttgccaac gcccggctcg
      721 ccgatcaaga cgggattatt cttctgacgg cgacccagca ccgtaatcaa acggcgcaat
      781 tcgcgcgccc tccctgctgc cgggtcgagt tttccagcag cggcctcggc ggttagatcg
      841 cgtccatatt gatcgagcgt gggggttttg ctatgtttgg cctgctgctg ggcggtcgct
      901 gtattgcgac cactccgcga gaagacagga ttatctgaat tgtcgttgtt tttcccaaca
      961 aacccaaacg gagcgccatc tggtaaaccg gagctatcaa gaccgcccat ctggttcatg
     1021 gcatcgctca tcaactcatc gacgcacgat ttgcagaggt aatgctgctc gcgctttcca
     1081 ttgatgatct ggtcgatacg cacctgggcg ggattctgtc cacagcgttc gcatttcatg
     1141 aatttgtatg ctcctgacta agtgtaattg attgccgaac tcttacttct ctcatcggtt
     1201 gcccgcgccg cctctcaaga gggtccacgc gcttcatcaa aaagtagagc atcgagaagt
     1261 cggctgctat ctagcagtct atccatgcga ttgccaagag tatatcacag aacattctgg
     1321 ttgtctagca gtcagcagag aagattgcta attaccaact aaaacaccac agatcagact
     1381 gttattccag aaatgacggg caattgtgaa gtttcccgac caggttcagt tctgtcaccc
     1441 tgagcgcagc gaagggtcta tttttcagcc atgaattgct atgttaattc ccaccatacc
     1501 cctcgttata tgggtagcaa agtaattctt gcgtgctttt caaaaggcca gcggagagga
     1561 agcatgccag gatgcagttg tttgttagtg atgtatacta aagaaaaagc gagggaaaga
     1621 aaatgccata cgattcctct tcagcaggcg gaatccttgc agcagtaggg ggcgtcacac
     1681 tgattgtgtt ccttgctatt gctgtattta ccgtcatcat ctattggaga atagtgtcta
     1741 aggccggtta taatggcgcg atgagcttgc tactgttcat ccccattgca aacctcatca
     1801 tgattatcat attcgccttc agcgaatggc ccatccagaa ggaactcaac caactgcgcg
     1861 cgatgtccgg acgcggcggc ccaggcggcc caggcagccc attcccttca tcgccgcaat
     1921 accctggcta tggacagccg caatatgggc agccggggca gccacaatat ggacaacccg
     1981 ggcagccacc gcaatatccg cagcgctaaa ataacccatc caaaactcaa cgctccgctt
     2041 actccccgta ggggcggggg aagacgcggt gtggggaggg gacgcttgcg tcgcccgcgt
     2101 accaggtgga tggaatcaat cgctggcagg ggaatcgcag atcaacgggt atgcgggcga
     2161 cgcaagcgtc ccctcccctc gtccgccctt cccccgcccc tacgggcttc tcctctatat
     2221 ggtatagtca atgagaaact gttctgccta tcaccagagg agaatgcgcg catcatggat
     2281 tttgcctaca ccgagaaagt caaccagctg cgcaagcgcg tcagcgactt catggaacgc
     2341 tatatttacc caaaccagtc cacctatcgc cagcagatcg aggcatcggg cgacccgcac
     2401 caccacgctg agatcaccga cgaactgaaa gccatcgcca ggtcggaggg tctatggaac
     2461 ctgttcatgc ccgatgacga gtatggcgcg ggactgacca acctggaata cgcgcccctg
     2521 gcggagatca tggggcgcgt tagctggtca tccgaggtct tcaactgcgc cgcgccagac
     2581 accggcaata tggaaatcct ggcgcagttc ggcacgccag agcagaagcg tgcgtggctc
     2641 atgccgctgc tcaacggtga gatccgctcg gccttcgcca tgacggagcc ggaggtcgcc
     2701 agctcggatg ccaccaatat tcagttgacc atccggcgcg aaggcgatga atatgtcctc
     2761 aacgggcata aatggtggat atcgggcgcg gcgcgcgcgc gctgcaaaat cttcatcgtc
     2821 atgggcaaga ccgcccccga cgcgcccgat acgcaccggc aacagagcat ggtgctcgtc
     2881 cccaaagata cccccggcct gaccatcgtg cgcaacctgc ccgttatggg ctacatcgat
     2941 aacgaaagcc actgcgagat gctcttcgag aacgtgcgcg tccccgtcga caacctgctc
     3001 ggtgaggagg gcggcggctt cgccatcgca caggcgcgcc tgggacccgg gcgcatccac
     3061 cactgtatgc gcgccatcgg cggggcgcag tatgccctgg agctgatgtg caaacgcgcc
     3121 gcctcgcgtg tggctttcgg caaacccctc ctggatcagg gcgtcatcca ggaatggatc
     3181 gcccaatccg aactggagat cgagcaggca cgcctgctga cgctcaaagc cgcctggatg
     3241 atcgacacgc tcaacaagaa ggccgcgcaa aaagagatcg ccatgataaa gattgccgcg
     3301 cccgaagtct tcactaacgt ggtcagccgc gccatgcagg tcttcggggc cgccggcctc
     3361 agcgacgatt tcccgctggc ctccatgtgg gcgcacgggc gcaccctgca tatcgccgac
     3421 ggccccgacg aagtacacaa acgagccgta gcccgcatcg tcatccgcca attggcgcga
     3481 taaagttgat gtggcgtgta ggggcgccat tcattgcgcc ctcgttccgg gtcacacgcg
     3541 caatggagcc agcacacttc tccggccaaa ccctcgtcct gctcaatccg gcc
//
LOCUS       DASZAK010000246         1706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_182969, whole genome shotgun sequence.
ACCESSION   DASZAK010000246 DASZAK010000000
VERSION     DASZAK010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182969"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..695)
                     /locus_tag="VGT44_04130"
     CDS             complement(<1..695)
                     /locus_tag="VGT44_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HEV2580020.1"
                     /translation="MDLMEKLASIARRYDELNRLLAQPEILEDISLLQSYGREHAELE
                     EVVANYHELQANEQQLADAQELYETSDDAEMRQLAYEEIQHLKEHKERLLEQVKVTLL
                     PKDIMDDKNAIVTIQGGAGGDEAALFAAELFRMYTRYAESKRWKIEILDSNETEQGGI
                     KDITFVVRGRGAYSRMKYEGGTHRVQRVPVTEANGRIHTSTAKVIVLPEVDDEINIDI
                     KDSDIRVDVYRSSG"
     gene            793..>1706
                     /locus_tag="VGT44_04135"
     CDS             793..>1706
                     /locus_tag="VGT44_04135"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580021.1"
                     /translation="MFTTYLTLEEDESMRFCFILEEKYVNKPMPMVVADQLQQWGHEI
                     EVLQPYAAVTSLTDLSLTNYDAYVLKTVSDGPGLTILEAAEAVGIPTINNSRAIRLVR
                     DKAVSVAYAQACGLPVPTTYFVGHYRALMQVPHDLYPVVVKPSNGTSLQDIHKVRTPE
                     EMAQLKLDETSYYLAMRYVENTGYDIKLYITGQEVHAVVKSSPLHGIVQEKEITVDRE
                     MLKLARRVGEVFGLDLYGVDVLETPDGLMLVDINDFPSFYGVPRKVASVAEYILHAAY
                     RNRLHKKRLARPRITTRKSLGRQDTRIVP"
BASE COUNT          337 a          507 c          453 g          409 t
ORIGIN      
        1 ccgctcgacc ggtagacatc gacgcgaata tcggaatctt tgatgtcgat gttgatctcg
       61 tcgtcaacct ctggcagcac aatcaccttc gccgtcgagg tatggatgcg cccgttggcc
      121 tccgtgacgg ggacgcgctg cacgcggtgc gtgccgcctt cgtacttcat acggctgtac
      181 gcgcctctgc ccctgacgac gaacgtgata tccttgatgc cgccctgctc ggtctcgttg
      241 ctatccagaa tttcgatttt ccagcgtttg ctttcagcgt agcgcgtata catacggaag
      301 agctcggcgg cgaagagcgc cgcttcgtcg ccacccgcgc cgccctggat tgtgacgatg
      361 gcgttcttgt cgtccatgat atccttcggc aggagcgtga ccttgacctg ttcgagcagt
      421 cgctccttat gctccttcaa atgttgtatc tcttcataag caagctggcg catctctgcg
      481 tcgtcgctgg tttcgtacaa ttcctgcgcg tcggccagct gctgctcgtt tgcttgcagt
      541 tcgtgatagt tcgccacaac ctcttcgagt tcggcgtgtt cgcggccata gctctgcaac
      601 agcgagatat cttcgagtat ctccggctga gccagcaagc gattcaattc gtcatatcgc
      661 cgcgcaatgc tggcaagttt ctccattaag tccataaaag atgaattctc ccttgatctt
      721 cctcgtcttc ttcatcatat cacatttttt gtgaagcacc tcctattctt ccaggccgat
      781 taggagtata ctgtgttcac aacatacctt acactggagg aggacgagtc gatgcgcttc
      841 tgctttatcc tggaagagaa atatgtcaat aagccgatgc cgatggtcgt ggccgaccag
      901 ttgcagcagt gggggcacga gatcgaggtc ttgcagccct atgccgccgt caccagcctg
      961 accgacctgt cgctgacgaa ctatgatgcc tatgtgctga agactgtttc ggatgggcca
     1021 gggctgacga ttctggaggc tgccgaggcg gttggcattc ctaccattaa taattcgcgc
     1081 gcgatccgcc tggtgcgcga caaagctgtc tcggtggcct atgctcaggc gtgcggcctg
     1141 cctgtgccta ctacctattt tgtcgggcat taccgcgccc tgatgcaggt gccgcacgac
     1201 ctctaccccg tcgtggtcaa accaagcaac ggcacctcgc tgcaggacat ccacaaggta
     1261 cgcactccgg aggagatggc gcagctcaag ctggatgaga cgagctacta ccttgccatg
     1321 cgctatgtcg agaataccgg ctacgatatc aagctctaca tcaccggcca ggaagttcac
     1381 gccgtcgtca aaagctcacc actacacggc atcgtgcagg agaaagaaat taccgtcgat
     1441 cgcgagatgt tgaaactggc gcgccgcgtc ggagaggtct tcggcctcga tctgtacggc
     1501 gtggatgtgc tggagacacc agatggcctg atgcttgtcg atatcaacga tttccccagc
     1561 ttctacggtg tgccgcgtaa ggttgccagc gtggcagaat atattctgca cgcggcttat
     1621 cgcaaccgcc tgcacaaaaa acgcctcgct cgccccagaa ttacgacccg caagtcgctt
     1681 ggccgccagg atacccgcat cgtccc
//
LOCUS       DASZAK010000247        12426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_646008, whole genome shotgun sequence.
ACCESSION   DASZAK010000247 DASZAK010000000
VERSION     DASZAK010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646008"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VGT44_04140"
     CDS             complement(<1..727)
                     /locus_tag="VGT44_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV2580022.1"
                     /translation="MSLAEQPQGFAEEAVRKYGYRQEFRREIKRFASFAIGFSFISIT
                     TGIFTTYGYVLANSGPLGIWTWPVVIVGQLFVALVFAALASRLPLAGYSYQWTSRLAN
                     PYIGWLIGWISFVFLIVDVVAVDYAVASTVLPSLFGYTGTPQNTWLATAIVVFIQMLL
                     IVFSTLWSSRINSTAVATELIGIIGLTLLLFIVGAVRGLLHPDHVFSTGVVSSSGWLS
                     LGSWTSSGPFMLAFLLGAFTIVGF"
     gene            complement(790..1239)
                     /locus_tag="VGT44_04145"
     CDS             complement(790..1239)
                     /locus_tag="VGT44_04145"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV2580023.1"
                     /translation="MKLNGSVAVDAPQEQVWRVFIDPAQLCKIIPGCEQARQLDDTHY
                     EALLSVKVQFMTIRSKARGTLLEADAPRHMVGELVGEPLTMAGAFRMRVTIDLLPQGN
                     ATLVRYAMDLTLLGRLASLGEAMVRATSQQLTRQFAENIAAQFGSHS"
     gene            complement(1236..2084)
                     /locus_tag="VGT44_04150"
     CDS             complement(1236..2084)
                     /locus_tag="VGT44_04150"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2580024.1"
                     /translation="MNAVYDLAIVGAGLSALSAIAAGLGDQQVVVFDYQDAPGGFLQH
                     ALPAQGFETAWDLMRSVRFPASFSVNCGATAVGLLPAFDEGAAHSLLVRRRQGTDEAK
                     ARRILIACGGLELTREQAQIPGPRPAGVMTPILAHQLLARGYLPGRRAIVYGDARYTR
                     ATARRMSEAGIEVTLVTPDEAELVAIEGFPRLERVTFRRDEAIFQAAADSFVYGVGMM
                     AHTHWLAGSGIELSPRGAIAVDERYRTNIPGIYAIGTVVAPSLDHTGSIMMGKEVALH
                     LQGGIP"
     gene            complement(2068..3507)
                     /locus_tag="VGT44_04155"
     CDS             complement(2068..3507)
                     /locus_tag="VGT44_04155"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2580025.1"
                     /translation="MQPEIRRVDILVIGGGVVGCAILRECSRYQASVALLERNPDICD
                     GTSKANSAIVHTGFDAPPGTLEARLLAGARVLWPQVIQSLHIPFVQTGALMVATSAEE
                     LGILENEIIPKAERNGVALQRLTREDILEHSPYVNASVLGGALVEGEGIIDPFWTTRA
                     YCENAVVNGAQVFTGEAVTAMSVEEQRVSVQTGGGLSFSASMVVNAAGLWSDEVAHLA
                     GDDSFTLTPRKGQFLVVEQDHGVSQIILPVPTRISKGILVTPIVFGGVMLGPTAEEVE
                     SKADFATTSAGMEQIRRGVGKLVPAMADAVSVRQFAGLRAVSSTGEYIIRPSSVGARL
                     LHVAGIRSTGLSVSPAIGRYVGNLVQAELGLTLKDTFIEELPEYLANAQADEGDVICL
                     CRSITRGEVMAALRSPLPPTTLDGLKRRTGAMLGECQGNLCLPRLIDLFQQQLGRDPL
                     TLDKHAANSSPVVARASGSGQGGSHERRL"
     gene            3857..4489
                     /locus_tag="VGT44_04160"
     CDS             3857..4489
                     /locus_tag="VGT44_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV2580026.1"
                     /translation="MLKQETTLVHIQFHTDPACPLAWRTALWIREARMVRPIDISWRF
                     FSLEVVNRQEGVTPDYARDGSWIAHRSMALVRHKYGNEAVERLYLALGNARHGQGENI
                     RLAEVVRDVMKRADLDPALVDAALEDETTIEDVLNDHQEAVRRYRAFGVPTIAINGSD
                     VGFYGPIIQNVPRGEEAGELWDYTAWALRQPNLFEMKRDRSGTHFGPISA"
     gene            complement(4509..5831)
                     /locus_tag="VGT44_04165"
     CDS             complement(4509..5831)
                     /locus_tag="VGT44_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV2580027.1"
                     /translation="MSDLDSVPEIALPVLDDRTGWAARWQALGQPWRIEPEISLERQR
                     HLAERRLITPDIIQGVYPFKDIALTRADVEWLLATHEDGRGPVDWSDERQRGREGLDL
                     RGADLRRVDLQNLPLACTVADVTWSRYVDLTDEQHFMAAIHLEGADLKGAHLEGARLE
                     FAHLDGADVRNAHLEQATLGRAFFIGAYMASAHLEGAGFYSAHLDEAFLWDAYLQGAV
                     LISTHLEAARLDRVKLADAQGVAARLIDIRWGDVNLGVVDWSQVKMLGDEHIARQSEQ
                     DGKIKDNATRLAEYETAVRANRQLAVVLQGQGLNEISTYFAYRAQVLQREVLRRRRKA
                     GQFLFSGFLDILAGYGYKPGRSVVAYLLVIAAFALAYALLGHTVHPVITPLGAVIFSV
                     TSFHGRGFFPGGIPLDDPITVFAAAEAVIGLIIEISFIATFTQRFFGK"
     gene            complement(5844..6077)
                     /locus_tag="VGT44_04170"
     CDS             complement(5844..6077)
                     /locus_tag="VGT44_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580028.1"
                     /translation="MPENQTSGLYTRSEDIPDERFRFFCSECGEPIKGIPYLTTWHGW
                     CACFDPWDFCSEECWNKNDLLNTANWPPPKGSW"
     gene            complement(6086..7264)
                     /locus_tag="VGT44_04175"
     CDS             complement(6086..7264)
                     /locus_tag="VGT44_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2580029.1"
                     /translation="MDTLKRELLAQRVRQVRPSGIRKFFDILNTMPNVISLGVGEPDF
                     VTPEHIRRAGIRSIELGHTQYTSNYGMLELRQEISAMLQRRYGLQYDPKSEILVTVGV
                     SEGVDVTMRTLIDPGDEVISPDPGYVAYEADIIFAGGKPVPVPTYARYGFSVRASEIA
                     AAITPRTKMILLGNPNNPTGAVVPRAELEGIAALARQHDLVVATDEVYSRLVYDTEHV
                     CIATLPGMRERAILLDGFSKAYAMTGWRIGYVAAPAHILEAMLKVHQYAIMCAGTAPQ
                     EAALEALRNGEDDVRMMHDSYDTRRRLFVAGLNRIGLPTCEPLGAFYAFPDITSTGMT
                     DEEFSEKLLFEEQVAVVAGSSFGEAGRGYIRCAYCTAQEKLEEALVRMERFIKKHKQP
                     "
     gene            7335..8279
                     /locus_tag="VGT44_04180"
     CDS             7335..8279
                     /locus_tag="VGT44_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEV2580030.1"
                     /translation="MSQSPPAPTQAADQSRRAPFPVLIDTDIGDDIDDAFALALALRS
                     PEIELHGVTTVFGDTRLRARLARHLLHIYGRDNIPVAAGESTPLLARHRPSGVPQAAI
                     LDPCEVLPISSYSGPKLIVQKAQAFPGQLTLICIGPLTNIADALRIDPHLYTAIRSIV
                     MMGGTSGLPSPEWNVRSDARAAQVVLASGIPITLLGWNITRRCQLQPADIARLSDCET
                     QQARLLSQLLAVWQRHRPRCQTAYPELHDPLTIAAICAPELLRFEEMAARVLVNAPPM
                     LRGVMVPRLMGGPLVRAVVGIQANKAREWVMGRLLTEA"
     gene            complement(8290..8865)
                     /locus_tag="VGT44_04185"
     CDS             complement(8290..8865)
                     /locus_tag="VGT44_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2580031.1"
                     /translation="MILRRFTADDVDLLVDLDSDPEVMRFITGGRPTSREVIQNDTLP
                     AFLRYVPVSGMGVFAAIEKESGEFLGWFSFRPKDAASPNDVALGYRLRRVAWGRGYAT
                     EGARALIRKGFTELGVQRVFATTYQDNLASRRVMEKAGLTLVRTYRMTVAELLANDTA
                     SHDPSEELWDGDDVEYALLKTDWEQQASNSI"
     gene            complement(8928..10076)
                     /gene="nifS"
                     /locus_tag="VGT44_04190"
     CDS             complement(8928..10076)
                     /gene="nifS"
                     /locus_tag="VGT44_04190"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HEV2580032.1"
                     /translation="MSDRVIYLDHAATTALDPRVLDVMLPYFTTEYGNASSIYTLGRH
                     AMQAIDGAREQVADLVGCRPTEITFTGGGSESDNLAIKGVAFTSQKKGQHLITSSIEH
                     HAVLHTCQYLERFGFRTSYLPVDGYGRVDPDDVARAITGETVLVSVMYANNEVGTIEP
                     IAEIGRICRARKIPFHVDAVQAGGALPIDVAALNADLLSLSAHKFYGPKGVGMLYTRQ
                     GVRLLPQLQGGSQERGKRAGTENVAGIVGAVAALRLAYEEFPQVTLRLVAMRDRLISG
                     VLTIPGAHLTGHPTDRLPNNASFMFEGIEGESILLSLDLHGIAASTGSACTSGSVDPS
                     HVLLAMGYSPEDARGTLRLTLGKDNTEADVDAVLAVLPGIVDKIRRLG"
     gene            complement(10084..11013)
                     /locus_tag="VGT44_04195"
     CDS             complement(10084..11013)
                     /locus_tag="VGT44_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580033.1"
                     /translation="MAKQSTRSRSKQGQANNKPSVKTNSATTNSSPVKTNDATAKTTG
                     GLSNGTSAKAATTPSVTPSTSAKAVSTQSKPSASGAAKQTQPAVPHRPSSKSKQQQRK
                     QQKLEHRLAQQQIDSKQRRNTIILVAAALVAVGGLLAYFFISNASAPAETVFNPDYPP
                     ISGVYCDQLEQTAYHHHVLLTIYIDGQNVQVPAGVGLAGSASSPTCYYWLHTHDTSGI
                     IHVEAPSGGSYSLQNFLSIWQSFANTSSTITYPSQLASSAGWTVYVNGKQVNEDFSHI
                     DISSNAAWHNLITIMYNSPSAKPVTTYSWQSGL"
     gene            complement(11038..11934)
                     /gene="larE"
                     /locus_tag="VGT44_04200"
     CDS             complement(11038..11934)
                     /gene="larE"
                     /locus_tag="VGT44_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEV2580034.1"
                     /translation="MPNTPSVNPPVEAPEALDTTALDVETRDKYRRLQEVLREMGSVL
                     VAYSGGVDSALLLKVARDVLGERAMGAIAASPAYADEETEEAIAVARQMGIPLLTLET
                     HELEDERYLANDLNRCYFCKTELFSQLAPLAKAHDLRNIAYGVNLDDDGDFRPGQRAA
                     REFGVRGPLKEAGMGKREIRAVAKLLGVPVWDKPAMACFSSRIPYGSRVDITSLNMVY
                     RAEKALRALGFRQVRVRHHATIARIEVERSELPRLVDEEISRQVTRSLREIGYLYVTV
                     DLQGYRTGSMNESFFKKKAKQS"
     gene            12059..>12426
                     /locus_tag="VGT44_04205"
     CDS             12059..>12426
                     /locus_tag="VGT44_04205"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV2580035.1"
                     /translation="MKTPNTTAAIVLAAGASTRMRGGRHKLLLPFGDRPVLARVLDAA
                     LASRARPIVLVLGHEAERVRAHIPTYVGHPDIIIVDNPAYLQGMSTSMKLGLQTLIDY
                     DYTKSGSGSVDSALFLTGDQP"
BASE COUNT         2474 a         3838 c         3716 g         2398 t
ORIGIN      
        1 caaagccgac gatggtaaac gcgccgagca ggaaggccag cataaacggc ccggacgagg
       61 tccaactgcc gagcgagagc cagccggagg aggagacgac gccggtgctg aagacatggt
      121 cggggtgcag cagcccgcgc acagcgccca cgatgaagag cagcagcgtc aggccgatga
      181 tgccgattag ctcggttgcg acggcggtgc tgttgatgcg ggacgaccag agcgtggaga
      241 agacgatcaa tagcatctgg atgaaaacca cgatggccgt tgccaaccag gtattctgcg
      301 gcgtcccggt gtagccgaag aggctgggca gcaccgtcga agccaccgcg taatcgacgg
      361 ccaccacgtc tacgatcaga aagacgaagg atatccagcc gatcaaccag ccaatgtatg
      421 gattggccag gcgcgatgtc cactggtacg aataacccgc caggggcaaa cgcgaggcca
      481 gtgccgcgaa gaccagcgcg acaaacagtt ggcccacgat gacgaccggc cacgtccaga
      541 tacccagcgg cccgctgttc gccagcacgt acccgtaggt ggtgaagatt cctgtggtaa
      601 ttgaaatgaa gctaaacccg atggcaaatg aggcaaagcg tttgatctct cgccggaatt
      661 cctggcggta gccgtatttg cgtaccgctt cttcggcaaa gccctgcggc tgttctgcga
      721 gtgacattga cctgctcctt tcgtgataga ttgaatgaat acaatgcgaa aaacgataca
      781 gaaggacaat tacgagtggc tcccgaattg agccgcgata ttttcggcaa attgccgggt
      841 gagttgctgc gacgttgcac ggaccatcgc ttcgccgagg ctcgccagtc ggcctaacag
      901 cgtgaggtcc atcgcgtaac gcacaagcgt agcgttaccc tgcggtagca ggtcgatggt
      961 aacgcgcata cgaaacgctc cggccatcgt gagcggctcg cccaccagtt cgccgaccat
     1021 gtgacgcggc gcatctgcct ccaaaagcgt ccccctggcc ttcgagcgga tcgtcatgaa
     1081 ctgcactttg accgaaagaa gcgcctcgta atgcgtgtcg tcgagctggc gcgcctgctc
     1141 gcagccggga atgatcttgc agagctgcgc cgggtcgatg aagacgcgcc agacctgctc
     1201 ctgcggcgca tcgacggcaa cgctgccgtt cagcttcatg gtatgcctcc ttgaaggtgt
     1261 aaagctacct cctttcccat catgatcgag ccggtatgat cgagcgaggg agcaacgacg
     1321 gtgccaatgg cgtagatgcc gggaatattc gtgcggtaac gctcatccac cgcaatcgcg
     1381 ccgcgcggcg acagttcgat gccgctgccc gcgagccagt gcgtatgcgc catcatgccg
     1441 accccataaa cgaatgaatc cgccgccgcc tggaagattg cttcatcgcg gcgaaacgtc
     1501 actcgttcca ggcgcggaaa gccttcgata gccaccagtt cggcctcgtc gggtgtcacc
     1561 agcgtgactt ctatccctgc ctcgctcatg cgccgcgcgg ttgcgcgtgt atagcgggca
     1621 tcgccgtaga cgatggcgcg cctgcctggc aggtagccgc gcgcgagcag ctggtgcgcc
     1681 agtataggcg tcataacccc ggcaggacgc gggccgggaa tctgcgcctg ttcgcgcgtc
     1741 agttccagcc cgccacacgc gatcaggata cgccgcgcct tcgcctcgtc tgtcccctga
     1801 cggcggcgca ccagcaggct atgggctgcg ccctcgtcga aggcgggcag caaacctaca
     1861 gcggtcgcgc cgcaattgac ggagaaggac gcggggaaac gcaccgaccg catcaagtcc
     1921 cacgccgtct caaacccctg cgccggaagg gcatgttgga gaaaaccacc gggcgcgtcc
     1981 tggtaatcga acacgaccac ctgctgatcc ccaagcccgg cagcgatggc gctcagggcg
     2041 ctcaagccag cgccgacgat ggccaggtca tagacggcgt tcatgcgacc ctccttgccc
     2101 tgaaccgctg gcacgagcaa ccaccggcga cgagtttgcc gcgtgcttat cgagcgtcag
     2161 tggatcgcgc ccgagttgct gctggaagag gtcgatcagg cgcggcaggc agaggttgcc
     2221 ctggcactcg cccagcatgg ccccggtacg gcgcttgagg ccatccagcg tggtcggcgg
     2281 cagcgggctg cgcagggccg ccatcacctc accacgcgtg atcgagcggc acaggcagat
     2341 gacatcgccc tcgtccgcct gggcattggc caggtattcc ggcagttcct cgataaacgt
     2401 gtccttcaac gtcagaccca attcggcctg caccaggttg ccgacatacc gcccgatcgc
     2461 tggcgaaacg gagagtcccg ttgagcgaat gcctgccacg tgcagcaggc gcgccccgac
     2521 gctggatgga cgaataatgt attcacccgt gctgctaacg gcgcgcagcc cggcaaactg
     2581 gcgcacgctg accgcgtctg ccatcgccgg cacgagcttg ccgacccccc ggcgaatctg
     2641 ctccatcccc gcggaagtgg tcgcgaaatc tgccttcgat tcgacctcct cagcggttgg
     2701 accaagcatg acgccgccaa agacgatagg cgtcaccagg atgcccttcg agatgcgcgt
     2761 cggcaccggc agaatgatct gcgagacgcc atgatcttgc tcgacgacca gaaattgacc
     2821 cttgcgcggc gtgagcgtga aggaatcatc acccgccagg tgcgctactt cgtccgacca
     2881 gagaccagcc gcgttgacga ccatcgaggc ggagaacgac agcccgccgc ccgtctgcac
     2941 gctaacacgc tgctcttcca cgctcattgc cgtgaccgcc tcgccggtga agacctgcgc
     3001 gccgttgacg acagcattct cgcaatacgc gcgcgtcgtc cagaacgggt cgatgatgcc
     3061 ctcgccttcg acgagtgcgc cacccagcac ggaagcattc acgtaggggc tatgttccag
     3121 gatgtcttcg cgcgtcaaac gctgcaacgc gacgccgttg cgctcagcct tgggaatgat
     3181 ctcgttctca aggatgccca gctcctccgc gctggtcgcc accatcagcg cacccgtctg
     3241 cacaaagggg atgtgcaggc tctggatgac ctgcggccac aacacccgcg cacccgccag
     3301 caggcgcgcc tcgagcgtgc caggaggcgc atcgaagccg gtatgcacaa tggcgctgtt
     3361 ggctttgctg gtgccatcgc agatatcagg gttgcgttcc agcagcgcaa cgctcgcctg
     3421 gtaacgcgag cattcgcgca ggatggcgca tcccacaact ccaccgccaa tcacgaggat
     3481 atctacccgg cgtatctctg gttgcatagc cattcgcttt cagcagatga ggtatgatgg
     3541 tttatgatga aagtgtacat aactgatgat gagttgtcaa cgtttttgag catcagtttg
     3601 aatcgttgcg atgtggcatc atcacaaatg tcaccctgag cgaagcgaag ggcctcggtt
     3661 gatgggccgc gagatgcttc gcttcgctca gcatgacaat cccgactttg atctgcaaag
     3721 ttcatcattg ttgcctcttc agtcttgaca tgcctctctt gctcgacgta tgttactcat
     3781 gaaggcactt cacaaactga agcattgcca gtccagctta ttcccccatc attctagaaa
     3841 gcgagacaac gacccgatgc tcaagcaaga gacgaccctc gtccatatcc agtttcatac
     3901 cgacccggcc tgcccgctgg cgtggcgcac ggcgctgtgg attcgcgagg cgcgcatggt
     3961 gcggccaatt gatatttcct ggcgattttt tagcctggag gtggtcaatc gccaggaggg
     4021 cgtgacgccg gattacgcca gagacgggtc atggatcgcg caccgctcga tggcgcttgt
     4081 gcgacataaa tacggcaacg aggcggtaga acggctctac ctggcgctcg gtaacgcgcg
     4141 gcatgggcag ggcgaaaaca tccggctggc cgaggtggta cgcgacgtga tgaaacgcgc
     4201 cgatctcgac ccggcgctgg tggatgccgc gttggaggac gaaactacca tcgaggatgt
     4261 gctgaacgat caccaggagg cggtgcgccg ctaccgcgcc ttcggcgtgc cgaccatcgc
     4321 tatcaacggc tcagatgtcg gcttctacgg ccccatcatc cagaacgtgc cgcgcggcga
     4381 agaggccggg gagctgtggg attataccgc ctgggcgctg cgccagccga acctgttcga
     4441 gatgaaacgc gaccggagcg ggacacactt cggtccgatc agcgcgtagc gcattacaat
     4501 taccgggatc acttcccgaa gaagcgctgc gtgaaggtgg caataaagct gatttcgatg
     4561 atgaggccga tgacggcttc tgctgcggcg aagacggtaa tggggtcatc taacgggatg
     4621 ccaccgggga agaacccgcg cccgtggaac gaggtaacgc tgaagatcac tgcgccaagc
     4681 ggcgtgataa ccgggtgtac tgtatggcca agcaaggcat aagccagggc gaaggcggca
     4741 atcaccagca gataggcgac gacgctccgc cccggtttat acccatagcc ggccaggata
     4801 tcaaggaatc ctgaaaaaag gaactgaccc gctttgcgcc tccgccgcag cacctcgcgc
     4861 tgcagcacct gcgcgcgata ggcgaagtag gttgagattt cgttaagccc ctggccttgc
     4921 agcacaaccg cgagctgtcg gttggcgcgc accgccgttt cgtattcggc caggcgcgtc
     4981 gcgttgtctt tgattttgcc atcctgctca gattgccgcg caatgtgttc atcaccaagc
     5041 atcttcacct gcgaccagtc taccacgccg aggttcacat cgccccagcg aatatcgatc
     5101 aagcgcgccg cgaccccttg cgcatcggca agtttgacgc ggtccaggcg cgcagcctcc
     5161 aggtgagttg atataagaac cgcgccttgt agataggcat cccagagaaa agcttcgtcc
     5221 aggtgagccg agtagaaacc agcgccttcc aggtgcgctg aggccatata ggcgcctata
     5281 aaaaatgctc taccgagagt tgcctgttcg aggtgcgcgt tgcgtacatc cgcaccgtcc
     5341 aggtgtgcaa attccaggcg cgcaccctca agatgcgcgc ctttgagatc ggctccttcc
     5401 aggtggatgg ctgccatgaa gtgctgctca tcggtcaggt caacatatcg actccacgtc
     5461 acatcggcga ctgtacacgc caggggcagg ttttgcagat caacacggcg caagtccgct
     5521 cctcgtaaat ccaggccctc ccgaccgcgc tggcgctcgt cgctccaatc gaccgggccg
     5581 cgcccatcct cgtgcgtcgc cagcagccac tcgacatcag cgcgagtcag cgcgatgtcc
     5641 ttgaagggat acacgccctg tatgatatcg ggagttatca aacggcgttc cgccaggtgg
     5701 cgctggcgtt ccaggctgat ctctggctcg atgcgccagg gctgccccag ggcctgccag
     5761 cgcgctgccc aacctgtgcg atcatccagt accggaagcg ctatctccgg cacgctatcg
     5821 aggtcactca tacctctcct cttttaccag cttcctttcg gaggaggcca gttcgcagta
     5881 ttgagcaggt cattcttgtt ccagcactcc tccgaacaga aatcccaggg atcgaaacag
     5941 gcgcaccagc catgccaggt cgtcaggtag ggaatgccct tgatcggctc gccgcattcc
     6001 gagcagaaga aacggaagcg ctcatccggg atatcctcgg agcgcgtgta gagaccggaa
     6061 gtttggtttt ctggcatacg tttccctacg gttgtttgtg cttcttgata aagcgttcca
     6121 tgcgtaccag ggcttcttct agcttctcct gggcggtgca gtaggcgcag cgtatgtagc
     6181 cgcgaccggc ttcgccgaag gaactgcccg cgacgacggc gacctgttct tcaaagagca
     6241 gcttttcgga gaactcttcg tcggtcatgc cggtggaggt gatatccggg aaggcgtaga
     6301 acgcgccgag cggctcgcag gtgggcaggc cgatacggtt gaggccagcg acgaagaggc
     6361 ggcggcgtgt gtcgtaggag tcgtgcatca tccgcacatc gtcttcgccg ttgcgcaggg
     6421 cctcgagcgc ggcctcctgc ggggcggtac cggcgcacat gatggcgtac tggtgtacct
     6481 tgagcatggc ctccaggatg tgcgcgggcg cggcaacgta gccgatacgc cagcccgtca
     6541 tggcataggc tttcgagaag ccgtcgagca ggatggcgcg ctcgcgcatc cccggcagcg
     6601 tggcgataca aacatgctcg gtatcgtaga ccaggcgact gtacacctcg tccgtcgcca
     6661 cgaccaggtc gtgctgccgg gcgagtgcgg caataccttc cagttcagcg cgcggcacaa
     6721 ccgcgccggt cgggttattg gggttgccga gcaggatcat cttggtgcgc ggcgtgatgg
     6781 cggcggcaat ctcagaggcg cgcacgctga agccgtaccg cgcataggta ggcactggaa
     6841 cgggtttgcc gcctgcgaag atgatatcag cctcgtaggc cacatacccc ggatcgggcg
     6901 aaatcacctc gtcgccaggg tcgatcaacg tgcgcatcgt cacgtccacc ccttcgctga
     6961 cgccgaccgt caccagtatc tcgcttttcg gatcgtattg cagcccatag cggcgttgca
     7021 acatggctga gatttcctgg cgcaactcca acatgccata attggaggta tactgggtat
     7081 gccctagctc gatagaacgt atcccggcgc ggcggatatg ctccggcgtg acgaaatctg
     7141 gttcgccaac gcccagcgaa atcacgttgg gcatcgtatt gagaatgtca aagaatttac
     7201 ggataccaga gggtctgacc tggcgcacac gctgcgccag caattcacgt ttgagcgtgt
     7261 ccatcgttga gttacccttt catgcaagtt gcttatgctt tacccaaatg taccatatgt
     7321 aggatgattt gccaatgagc caatccccgc ccgcgcccac acaagcagcc gaccaatcgc
     7381 ggcgcgcccc ctttcccgtc ctgatcgaca ctgacatcgg cgacgacatc gacgacgcct
     7441 tcgccctcgc cctggcgctg cgttcgccag agatcgaact gcacggcgtc accaccgttt
     7501 tcggcgatac gcgcctgcgc gcaaggctgg ccaggcatct cctgcatatc tatggccgcg
     7561 acaacatccc cgttgccgct ggtgagtcta ccccgctcct ggcgcgtcac cggccatccg
     7621 gcgtgccgca ggccgccatt ctcgacccct gcgaggtcct tcctattagc tcctatagcg
     7681 gccccaaact gatcgtacaa aaagcgcagg cattccccgg ccaattgacc ctgatctgta
     7741 tcggcccgct cactaacatc gccgacgcac tgcgcatcga tcctcatctt tatacggcta
     7801 taagaagtat cgtcatgatg ggcggcacca gcggcctgcc ctcgcccgaa tggaatgtgc
     7861 gcagcgacgc cagggccgcc caggttgtgc tggcatcggg catccccatc accttgttgg
     7921 gttggaatat caccaggcgc tgccagctcc agccagccga catcgcccgc ttgagcgact
     7981 gcgagacaca gcaggcgcgc ctgttgagcc agttgctggc cgtctggcag cgtcaccgcc
     8041 cacgctgtca aaccgcctat cccgagttgc acgacccctt gaccatcgcc gccatctgcg
     8101 cgccagaact gctgcggttt gaggagatgg cagcacgcgt actggtgaat gcgccgccga
     8161 tgctgcgcgg tgtgatggta ccacgcctga tgggtgggcc gctggtacgc gcggtggtcg
     8221 gcattcaggc gaataaggcg agggaatggg taatggggag gttgttaaca gaggcgtgaa
     8281 ggcaccctgt catatgctgt ttgaagcctg ctgctcccag tcggttttca gcaacgcgta
     8341 ctcgacatcg tctccgtccc aaagctcctc cgatgggtcg tgggaagcag tgtcattggc
     8401 cagcagttcc gcaaccgtca tgcgataggt ccgcaccagc gtcaggccgg ccttctccat
     8461 gacccggcgg gaggcaaggt tgtcctggta ggtcgtggca aacacgcgct gcacacccag
     8521 ctcggtgaag cccttgcgga tcaacgcgcg cgcgccttcg gtggcgtagc cccggcccca
     8581 ggcgacgcgg cgcagccgat agccgagcgc gacatcgttt gggctggccg catcctttgg
     8641 acggaagctg aaccacccca ggaattcacc gctttccttc tcgatggctg caaagacacc
     8701 cataccggag acaggtacat agcgcaggaa cgcaggcagg gtgtcattct ggatcacctc
     8761 gcgggacgtg ggtctgccgc ccgtgatgaa gcgcatgacc tctggatcgc tgtcgagatc
     8821 gaccaggagg tccacatcgt ccgctgtgaa gcggcgcagt atcattcgtt cggtttccag
     8881 gaaatcgcct cctccctccc agtgccatac attggaaaag ccactgctta ccccagccgc
     8941 ctgatcttgt ccacaatccc cggcagcacg gccagcacgg cgtctacatc ggcttcggtg
     9001 ttgtctttgc ccagagtcag gcgcagggtg ccgcgcgcat cttcggggga gtagcccatc
     9061 gccagcagca cgtgcgacgg atcgaccgag ccggaggtgc aggcggagcc ggtggaggcg
     9121 gcaatgccgt gcaggtcgag gctgagcagg atcgattcgc cttcgatgcc ctcaaacatg
     9181 aagctggcat tgttgggcag gcggtcggtg ggatggccgg tcaggtgcgc gccaggaatg
     9241 gtcagaacgc cggagatgag gcggtcgcgc atggcaacga gacgcagcgt gacctgtgga
     9301 aattcttcgt aagccaggcg cagagcggca accgcgccta cgatgccggc cacattttcg
     9361 gtgcctgcgc gcttgccgcg ctcctgcgag ccgccctgca actgcggcaa caggcgcacg
     9421 ccctggcgcg tatagagcat tccaacgccc tttggcccgt agaatttatg ggcggaaagc
     9481 gagagcagat cggcattcaa agcggccacg tcgattggca gcgcgccgcc cgcctgcacc
     9541 gcgtcaacgt ggaaggggat tttgcgcgcg cggcagatgc gcccgatttc ggcgatgggt
     9601 tcaatcgtgc caacctcgtt gttggcgtac atgaccgaga ccagcacggt ttcgcccgtg
     9661 atggcgcgcg ccacgtcatc gggatcgacg cggccatagc catcgaccgg caggtagctc
     9721 gtgcgaaagc cgaaacgctc aaggtactgg caggtatgta gcacggcatg atgctcgatg
     9781 gacgaagtaa tgaggtgctg gccctttttc tgcgaggtaa aggctacccc cttgatggcc
     9841 aggttatcgc tttccgagcc gccgccggtg aaggtgatct ccgttggccg gcagccgacc
     9901 aggtctgcta cctgctcacg cgcgccatcg atggcctgca tcgcgtgacg acccagcgtg
     9961 tagatgctgc tggcgttgcc gtactcggtg gtgaagtacg gcagcatcac atccaacacg
    10021 cgcgggtcga gcgcggtggt cgcggcatgg tcaaggtaga tgacgcgatc tgacatggct
    10081 gtcctataat cctgactgcc agctataggt cgtgaccggc ttggcgctcg ggctgttata
    10141 cataatcgtg atcaaattgt gccaggccgc gttgctggaa atgtcgatat ggctgaaatc
    10201 ctcgttgacc tgcttgccgt tgacgtagac cgtccagccg gctgacgagg ccagttgaga
    10261 cgggtaggtg atcgtgctac tcgtattcgc gaagctttgc cagatcgata ggaagttctg
    10321 gagcgaatac gaccctccgc tcggcgcttc gacgtggata atcccgctcg tgtcgtgggt
    10381 atgcagccag taatagcagg taggcgacga cgcgctgccc gccagtccga cacccgcggg
    10441 cacctggacg ttctgtccat cgatataaat ggtcaggaga acgtggtgat gatacgccgt
    10501 ctgttccagt tgatcgcaat agacgccgct gatgggcgga taatccgggt taaagaccgt
    10561 ctcagcgggg gcggacgcat ttgagatgaa gaagtaggcg agcagcccgc cgacggcaac
    10621 taaggcagcc gctaccagaa tgatcgtgtt gcgccgctgt ttcgagtcaa tctgctgctg
    10681 cgcgaggcga tgttctagtt tctgctgctt gcgctgctgc tgtttcgact tgctcgaagg
    10741 tcgatgggga acagccggtt gagtctgctt tgccgcgccg cttgcggaag gcttcgactg
    10801 cgtcgagacc gccttcgccg atgtcgatgg ggtcacggac ggagtcgtcg ccgccttcgc
    10861 cgatgtgcca ttcgacaagc cgccggttgt ctttgcggtt gcgtcattcg ttttgacggg
    10921 cgagctatta gtggttgcgc tgttcgtctt aacggatggt ttattgtttg cctgtccctg
    10981 ttttgatcgt gagcgtgtgc tctgcttggc cattgatgat cccaactttc ccaatactta
    11041 tgattgcttc gccttcttct taaagaaaga ctcgttcata ctgcctgtgc gatatccctg
    11101 caaatcgacg gtgacgtata aatagccaat ctcgcgcaga ctgcgtgtaa cctggcggct
    11161 gatctcttcg tcaaccaggc gcggcagttc ggagcgttcc acctcgatgc gcgcgatcgt
    11221 cgcatgatgc cgcacgcgca cctggcgaaa ccccagcgcg cgcagcgcct tttccgcccg
    11281 gtacaccata ttcagcgagg tgatatcgac ccggctgcca taggggatgc gcgacgaaaa
    11341 acaggccatc gcgggcttat cccacaccgg cacaccaagc agcttcgcca ccgcgcgaat
    11401 ctcgcgtttg cccatgcccg cctctttgag cggaccgcgc acgccgaact ccctcgctgc
    11461 gcgctgcccc ggacgaaaat cgccatcgtc gtccaggttc acgccatacg caatattacg
    11521 caaatcatgc gcttttgcca gtggcgcgag ctgcgaaaat agctcggtct tacaaaaata
    11581 acaacgattg aggtcattcg ccaggtaacg ctcgtcctca agctcgtgcg tttcgagcgt
    11641 cagcagggga atacccatct gccgcgctac ggctatggcc tcttcggtct cctcgtccgc
    11701 gtaggccggg gaagcggcaa ttgcgcccat ggcgcgctcg cccagcacat cgcgcgccac
    11761 cttcaagagc agcgcgctat cgaccccacc cgaataggct accagcaccg accccatctc
    11821 gcgcaggacc tcctgcaagc gtcgatattt gtctcgcgtc tccacatcca gcgcagttgt
    11881 atccagggct tccggcgcct caaccggagg attgacggag ggggtattgg gcatgttaca
    11941 gtagtctcct gaccagggtc gtgtacaata gataaggcgc gaacaccggc aaatgttccg
    12001 gctttgcgct ttactcaaca tgtattataa cattcacaca caagaggact gagaattagt
    12061 gaagacaccc aatacaaccg ccgccatcgt cctcgccgca ggcgcctcaa cccgcatgag
    12121 aggcggtcgc cacaagcttt tgctaccgtt tggcgatcga ccggtgctgg cgcgcgtgct
    12181 tgacgcggcg ctggcttcgc gggcgcggcc catcgtactt gtactgggtc acgaggcaga
    12241 acgagtgcgc gcgcacattc ctacatatgt tgggcatccc gatattatca tcgtcgataa
    12301 ccccgcttat ttgcagggca tgagtacgtc gatgaaactg ggattacaaa cactcataga
    12361 ttatgactat acgaaatccg gttcgggttc ggtggacagc gcactctttc tcacaggcga
    12421 tcagcc
//
LOCUS       DASZAK010000248         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_610315, whole genome shotgun sequence.
ACCESSION   DASZAK010000248 DASZAK010000000
VERSION     DASZAK010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610315"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VGT44_04210"
     CDS             complement(<1..620)
                     /locus_tag="VGT44_04210"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2580036.1"
                     /translation="MLDRSGQQFGKYRLLRLIGRGGFADVYLGEHVHLKNQSALKLLH
                     NQLSGVDIDAFLKEAQTIARLDHPHIVRIFDFDVQDGIPFLVMGFAPNGTLRDRYPKR
                     TLLPLSAIVSHVTGIADALQYAHNEKVVHRDIKPENMLLGRRNEIMLSDFGIAIVSQS
                     SRYQSTQNIAGTMGYMSPEQIQAHPRPASDQYSLGVVVYEWLSGQLP"
     gene            complement(754..2013)
                     /gene="rho"
                     /locus_tag="VGT44_04215"
     CDS             complement(754..2013)
                     /gene="rho"
                     /locus_tag="VGT44_04215"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEV2580037.1"
                     /translation="MNIAELETKTLTELRDIARDLELSGYTILRKQDLVFRLLQAHTE
                     QQGNIFSAGVLEIVEDGFGFLRQERFLPGNMDVYVSQSQIRRFGLRTGDMVSGQVRPP
                     KDNEKYYGLLRVEAINGMDPEVAKRRPHFDYLTPIFPREMFNLETTPSNISGRIINLV
                     APVGRGQRGLIVSPPKAGKTMLLKSIANSITENYDDVHLMIALIGERPEEVTDMKRSV
                     RAEVISSTFDEPTEAHTRVAEMVLERAKRLTEGGRDVVVLLDSITRLSRAYNLAVQPS
                     GRTLSGGLDPSALFPPKRFFGAARKIEEGGSLTVIATALVDTGSRMDDVIYEEFKGTG
                     NMELVLNRKLAERRVFPAIDIGLSSTRREELLLDDKTLRAVVVMRRMFSTLADQRGME
                     AMEALLQHMSKTSNNIEFLATLNKSIM"
     gene            complement(2314..>2714)
                     /locus_tag="VGT44_04220"
     CDS             complement(2314..>2714)
                     /locus_tag="VGT44_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580038.1"
                     /translation="DIPRVNQVEIAYGQPWKRRLGLIRLSFDNTRTFIGVNSLLQQSQ
                     APDCVLLTTIAHELTHYAHGFGSPLPRLFDHHHANNVVERDLERRGLGQMARQCDEWI
                     DNAWFAFYDTQQRIKLEARKIPATKILDTD"
BASE COUNT          585 a          694 c          819 g          616 t
ORIGIN      
        1 ggcaactgac cgctcagcca ttcatagacg acaacgccga gcgagtactg gtcgctggca
       61 ggacgtggat gcgcctggat ttgctcaggc gacatatagc ccattgttcc ggcaatattc
      121 tgcgtactct ggtagcgcga actctgcgag acaatggcga taccaaagtc gctgagcata
      181 atctcattac gcctgcccag taacatattc tcaggtttga tgtcacgatg aaccactttt
      241 tcattatgtg cgtactgcaa agcatcggcg atgcccgtca catgggatac gattgctgac
      301 aatgggagga gcgtcctttt agggtagcgg tcccgcaacg ttccgttcgg agcgaatccc
      361 atgacaagga acggaatacc atcctgcacg tcaaagtcga agatgcgcac aatatgtgga
      421 tgatccagtc gggcgattgt ctgcgcttcc tttagaaaag catcgatatc cacgcccgat
      481 aactggttat gaagcaattt aagggcggac tgattcttca gatgtacatg ttcgcccagg
      541 tagacatctg cgaacccacc acgcccaata agacgaagca gacgatattt gccgaactgc
      601 tgaccagagc gatccaacat agcccaaacg aacctccaca tagactgcta tctatttgaa
      661 cgcgaaatcc ggcattatgt aacgctggac agagcagcta agcgactctt gcttctcata
      721 ttaatgaact gtaggagcag agagcctgtg tttttacatg atgctcttgt tcagcgtggc
      781 caggaactcg atgttgttgc tggtcttgga catgtgctgg agcagggctt ccatggcctc
      841 catgccgcgc tggtcggcga gggtggagaa catacggcgc atgaccacga cggcgcgcag
      901 ggttttgtcg tcgagcagca gttcttcgcg gcgtgtgctg gacaggccga tgtcgatggc
      961 agggaagacg cggcgctcgg ccagcttgcg gttcagcaca agctccatgt taccggttcc
     1021 tttgaattct tcgtagatga catcgtccat gcggctgccg gtatcgacca gggcagtggc
     1081 gatgacggtc aggctgccgc cttcttcgat tttgcgggcc gcgccgaaga agcgtttggg
     1141 cgggaagagc gcgctgggat cgaggccgcc ggagagtgtg cgcccgctgg gctgcacagc
     1201 caggttgtag gcgcgactca ggcgcgtgat gctatcgagc aggacgacta cgtcgcgccc
     1261 gccttcggtg aggcgtttgg cgcgttccag caccatctcg gccacgcggg tatgcgcctc
     1321 ggtcggctcg tcgaaggtgg agctaatgac ctcggctctg accgagcgtt tcatgtcggt
     1381 aacttcttcc gggcgttcgc cgatcagcgc gatcatcaaa tgcacatcgt cgtagttttc
     1441 ggtgatcgag ttggcgatgg atttgaggag catggtcttg ccggctttcg gcggtgagac
     1501 gatcaggccg cgctgcccgc gcccgacggg ggctaccagg ttgatgatgc gaccagagat
     1561 gttggacggg gtagtttcca ggttgaacat ctcgcgcggg aagatggggg tcaggtagtc
     1621 gaagtgcggg cggcgtttgg cgacttcggg atccatgccg ttgatggcct cgacgcgcag
     1681 caggccgtag tacttctcgt tgtccttggg cgggcgcacc tggccggaga ccatatcgcc
     1741 ggtacgcagg ccgaaacgac gaatctgtga ttgagagacg tagacatcca tgttgccagg
     1801 caggaaccgc tcctggcgca ggaagccgaa gccgtcttcg acgatttcca gcactcccgc
     1861 actaaagatg ttgccttgct gttcagtatg agcctggagt agtcggaaaa cgaggtcttg
     1921 cttgcggagg atggtgtaac cggagagttc gaggtcgcga gcgatatcgc gtaactccgt
     1981 gagagttttg gtttcaagtt cagctatatt cacgttatgc cagggcgggt atgatcaggt
     2041 tgttctcgtg tggaacaacg cattttgagg gaacacgccc tttctccttt ctgtgttgaa
     2101 gctggagtgt agtatgcaaa gaaatagatt gatgtttttc ggaaaacgcc tgaaaaacgg
     2161 tggcaacgcg cattgcttga tgggtgaaag aggcatcgtc caacgtacct aagacagtta
     2221 cgacgcaaca aacaacatac acacagaact ctgcggaacc tgtaacatgg tgcggtctat
     2281 accttaggag ctagcatcac tataccacac gtatcaatct gtgtcaagga tttttgtggc
     2341 cgggattttc ctggcttcaa gtttgatcct ctgctgagta tcatagaacg caaaccaggc
     2401 gttgtcaatc cattcgtcgc attgccgtgc catttgaccc aggccgcgcc gttccagatc
     2461 gcgctcaacg acgttgttgg cgtggtgatg atcgaagagg cgcggcagcg gcgagccgaa
     2521 gccgtgtgcg tagtgcgtta attcgtgggc gatggtcgtc agcaggacgc agtccggcgc
     2581 ttgcgactgt tgcagcaggg aattcacgcc gatgaaggtg cgcgtattat cgaacgagag
     2641 gcggatcaac cctaatcgcc gcttccaggg ctgcccatag gcgatctcaa cctggttgac
     2701 gcggggaata tcag
//
LOCUS       DASZAK010000249         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_574374, whole genome shotgun sequence.
ACCESSION   DASZAK010000249 DASZAK010000000
VERSION     DASZAK010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574374"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..2415
                     /locus_tag="VGT44_04225"
     CDS             145..2415
                     /locus_tag="VGT44_04225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2580039.1"
                     /translation="MVDRTGQLIGNYRIVRLLGEGGFAEVYLGEHIHLGTEAAIKVLH
                     TQLAGEETGQFRAEARLIASLDHPNIVRVLDFGLAEKTPFLVMSYAPNGTLRQLHKRK
                     EPVPLSSIVQYVGQVADALFYAHQQRLVHRDVKPENMLIGRRNEILLSDFGIAFVSQS
                     SRYQGTHDIVGTVSYMAPEQIRGRPRPASDQYSLAVVVYEWLTGDRPFHGSFTEIAVQ
                     HTIAPPPPLREKIPGIAPEVEYVVLTALAKEPEQRFATIRAFANALQQASNVGGQTVY
                     DWQSTLVSPPPQPQRPLTPLPPTFTPLPPPPPSYPASFQQSGSQPPVILTPQGVSNTP
                     PGRYYQSSTDPNEPEPPRRGISRRTLLLGATALVLLAGSGATVLALAAQHLGSSATTP
                     TPGATPTTGGTTVPKVSPTSRTANHTPTPTTVPSTPSPSANGASPTTTPTVANTPTTT
                     PTTPTTPPPAKIVSTPLSTYNGHTNNVYAVTWASADQQRMASGGLDNTVLDWNRATNQ
                     LYFSYTQGLEINDVKASPSKQYIASASDDMTVKVYDASAGTSISTYSSHSSGVNTVRW
                     SSDGTLLLTSSTDQTARIWRLSDLATLHVLQGHTARVWAAAWSPDMKYAVTASADRTA
                     IVWDASIGQIISIYKGHSDALRAVSWSPRSTQIATASQDGTVQIWDGLTGAHILTYTG
                     HTGQVLSVEWSHAGTSIVSGSKDQTAQVWNPANGAQLLIFKGHTNSVFDAQWSPDDTL
                     IASASSDKTVQIWQSS"
     gene            complement(2426..3463)
                     /locus_tag="VGT44_04230"
     CDS             complement(2426..3463)
                     /locus_tag="VGT44_04230"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV2580040.1"
                     /translation="MKVLVTGAAGFLGGHLVDMLVERGDDVRAMVRPVEDSSRLRRLP
                     GVEVVQGDLTDAASLKRAVQGVECVYNVAARTGPWGLERDYNAINVWGVADLITAAME
                     AGARRIVHTSSITVYGHHLHGLVTEEHPYIAEDNPYSRTKIAGEKLIADMVKGRGAPV
                     VIVRPGWIYGPRDNASFGRFVALVDSGKGFIIGSGKNILPIVYVRDVAQGLILAGNAD
                     ERVVGRAYTLADDRRVTQGEYLNMIADFLDVPHLTRYYPYTALFGAGRVAELTWRAMG
                     RRKSAPPPVTTYGVTLLGGNQEFSIDRARNELGYEPQYDYIRGLSEGVKWYREKSQAA
                     AIAEREVAPAR"
BASE COUNT          762 a         1142 c          938 g          642 t
ORIGIN      
        1 tcggcggtga gcgcgataaa tcggctccta cgggtttgtt agcagttctt acgtgactgg
       61 cgcgaaaaaa acgggtgcct cgcgtatgat acaatagaag attagaacgt tcctcacctc
      121 ctatttatgg ggaaggtcat acctatggtt gatcgcacag ggcaactcat cggcaattac
      181 cgcattgtgc gcttgctcgg agaaggcggc ttcgctgaag tctacctggg cgagcatatt
      241 cacctgggaa ccgaagccgc catcaaggtg ctgcataccc aattggccgg cgaggaaacc
      301 gggcagttcc gcgcggaagc ccgcctgatc gccagcctgg atcaccccaa catcgtgcgc
      361 gtcctcgact tcggattggc cgaaaaaaca cccttcctgg tgatgagtta cgcgccgaat
      421 ggcacgcttc gccagcttca caagcgcaaa gaacccgttc cgctttcatc aatcgtgcaa
      481 tacgttgggc aggtggccga tgcgctgttc tatgcccacc aacaacggct ggttcatcgc
      541 gatgtcaagc cggaaaatat gctgataggg cgacgtaacg aaatcttgct gagcgacttc
      601 ggcatcgcct tcgtctcgca aagctcgcgc tatcaaggta cgcacgacat cgtcggaaca
      661 gtctcctaca tggcgccgga acagatccgg ggtaggccgc gccctgccag cgaccagtat
      721 tccctggcgg tcgtcgtcta cgagtggcta accggagatc ggccattcca cggctcgttc
      781 accgaaatag ctgtccagca taccatcgcg ccacctccgc cattgcgaga gaagattcct
      841 ggcatcgcgc cagaagttga gtacgtggtg ctgaccgcgc ttgccaaaga accagaacag
      901 cgcttcgcca caatccgagc ctttgccaac gcgcttcaac aggcgagcaa cgtgggggga
      961 caaacggtgt atgattggca atcgacactg gtttcgccgc cgcctcagcc gcagcgaccg
     1021 ctcaccccgc tgccgccaac attcacgccg ttgccgccac caccgccatc ctacccggct
     1081 tcattccaac agtccgggtc gcaaccaccg gtaatcttaa caccacaagg ggtgtcgaac
     1141 acaccgccgg gcaggtatta ccagtcctct accgatccga acgagcctga gccacccagg
     1201 cgcgggattt cacggcgcac gctactgcta ggcgcgaccg cgctggtgct gctggcaggc
     1261 agcggcgcaa ccgtgttagc gctggcggcg caacatctcg gcagtagcgc gaccactcca
     1321 acccctggcg caacaccgac caccggaggg acaacggttc caaaggtcag cccaacttcc
     1381 aggacggcaa accacacgcc aaccccgact actgtgccaa gtacaccgtc gccaagtgcg
     1441 aatggcgcct cgccaacgac cacgccaacg gtcgctaaca cgccgacgac tacaccgacc
     1501 acgccgacca cgccacctcc cgcaaaaatc gtcagcacgc ccctctcgac ctacaatggc
     1561 cacaccaaca atgtctatgc cgtcacctgg gcatctgccg accagcaaag gatggcttca
     1621 ggtggcctgg ataataccgt actggattgg aatcgcgcca ccaaccagtt gtatttcagc
     1681 tacacacaag gcttagagat caacgatgtg aaggcatcgc cttcgaagca gtatatcgcc
     1741 tcggcaagcg atgatatgac ggtaaaggtc tatgacgcga gcgctggaac ttccatctcg
     1801 acctattcca gccactcatc tggcgtaaac accgtgcgct ggtcgagtga tggaacgctg
     1861 cttctcacct ccagcacgga tcaaacggcg cgcatctggc ggctgtcaga cctggcaaca
     1921 ctacacgtct tgcaaggtca taccgcgcgc gtctgggcag cagcctggtc gccggacatg
     1981 aaatatgcgg tgaccgccag cgcggaccgc acagctatcg tctgggatgc cagcattggg
     2041 cagatcatct ccatctataa aggtcatagc gatgctttgc gagctgtctc ctggtcgccc
     2101 aggtctaccc agatcgccac cgccagccag gacgggacgg ttcagatctg ggatggtctg
     2161 accggcgcgc acatcctgac ctatacaggc cataccggcc aggtactctc ggttgaatgg
     2221 tcacatgccg gaacatccat cgtctcaggc agcaaggacc agacggcgca agtttggaat
     2281 cctgccaatg gtgcgcaact gctgatcttc aaaggccata cgaactcggt cttcgatgcc
     2341 caatggtcgc ccgatgatac gctgattgcc tcggccagtt ccgataagac cgtgcaaatc
     2401 tggcaatcaa gctaaacacg cctgtctacc tggcgggcgc gacttcacgt tccgcgattg
     2461 cagctgcctg gctcttctcg cgataccact tgacgccttc ggacagcccg cgaatgtaat
     2521 catattgagg ctcatagcca agctcgttgc gagcgcggtc aattgagaac tcctggttgc
     2581 cacccagcaa ggtgacgccg taggtagtga ccggcggcgg agcggacttg cgacggccca
     2641 ttgcgcgcca cgtcagttca gcgacgcgac ccgcgccaaa gagggcggtg tagggatagt
     2701 agcgcgtcag gtgcggcaca tccaggaaat cagcgatcat attgaggtat tcgccctgcg
     2761 tcacgcgccg gtcgtcggcc agcgtatagg cccggccaac gacacgctcg tcggcattgc
     2821 cggccaggat caacccctgc gccacgtcgc gcacatagac tatcggtaaa atattcttgc
     2881 ctgagccgat gataaagccc ttgccagaat cgaccagcgc cacgaagcgg ccaaagctgg
     2941 cgttatcgcg cggcccataa atccaacctg gccgcacgat taccaccggc gcgccgcgtc
     3001 ccttcaccat atcggcgatg agcttctcgc ccgcgatctt ggtgcggcta taggggttat
     3061 cctccgcgat gtagggatgt tcctcggtca caagtccatg caggtgatgc ccatacaccg
     3121 taatcgagct ggtatgcacg atacgccgcg cgcccgcctc catcgccgcc gtaatcaggt
     3181 cggccacgcc ccagacgttg atggcgttgt agtcgcgttc caacccccag ggacctgtcc
     3241 tggccgcgac gttgtagacg cactcaacgc cctgcacagc gcgtttcaac gatgccgcgt
     3301 cggtcaagtc gccctgcaca acctcaacgc cgggcagcct tcgcaagcgc gacgaatcct
     3361 ccacggggcg caccatggcg cgcacgtcat cgccgcgctc gactaacata tccacgagat
     3421 ggccgcctag aaaaccggca gcccccgtca ccagtacttt cactacgttc tactccttct
     3481 tgcc
//
LOCUS       DASZAK010000250         5136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_610486, whole genome shotgun sequence.
ACCESSION   DASZAK010000250 DASZAK010000000
VERSION     DASZAK010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5136
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610486"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VGT44_04235"
     CDS             complement(<1..1056)
                     /locus_tag="VGT44_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2580041.1"
                     /translation="MSRPGSPDRRCMMFRSIWSKSLRDYRVAILAWGFALGVFAAVDL
                     AESTPTTIATFSSLAPLFRFLGDPYQIQTPEGFITWRIMELFVPLAICFWPILAGARM
                     VRGEEERGSMNVLLATGRSRRRLLLSKLSALVIALILIGLLVALGLLAGQTRLEGHAD
                     VVRAVLAGLNASLLAIFFGSLALLFSQFVASRVVAAGWTSGLLVLSMLLDSTGRLLHG
                     SWVQYLSPFYYYNLNRPLIPSFPNQPLAAVLLAGLTVLCAGASLLLFARRDIGGVAFS
                     WPGQQEPGNHDAASRLSQAERALSTRNVGLQTLRRDGWSAIWWGIGVVFVCAYCLVIA
                     LSTQQALYRLIQETPQLQ"
     gene            complement(1031..1939)
                     /locus_tag="VGT44_04240"
     CDS             complement(1031..1939)
                     /locus_tag="VGT44_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580042.1"
                     /translation="MIQTNRLTKSYGRSRGILDVTFAIQEGEVFGFLGPNGAGKTTTM
                     RVLMGLLHANSGSATIGGLDCWAQSTEVKQLVGYLPGEFTFDPGLRGAQIIEYLAHLR
                     GGVDQDYLRSLVERLDLDPSKRFRDYSHGNKQKIGLVQAFMHRPRLLILDEPTSGLDP
                     LNQQEFYHLVAAVRASGRTVFLSSHILPEVEHTCDRVAIIREGRLIQIDHVSALKDIH
                     QYEVEISFAGPASVDWFKQVAGVANVAEAADERTLQLAVQGGLTEIIHIASQHNATNI
                     TTHEPTLEEVFLRFYESEHEQARLAG"
     gene            complement(1936..2571)
                     /locus_tag="VGT44_04245"
     CDS             complement(1936..2571)
                     /locus_tag="VGT44_04245"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2580043.1"
                     /translation="MQAQQAILDATLTFLATEGFDAMSIEAIAAKAGVGKQTIYRWWA
                     SKEALVIDAIKSWQQTQTPVIDTGSLREDFLVMTRSAFQAWNGSDARGLAMNILGVMT
                     THPHVYQAFYDQVVAPRFQQLAEMIRRAQAHGEVRQDLDANEIIGLLAGPLWYHLFLD
                     TDSIQLPHDLPERLVDAILEGIAMRRKPEEGDATLHDARETLAEKGNACQE"
     gene            2814..4085
                     /locus_tag="VGT44_04250"
     CDS             2814..4085
                     /locus_tag="VGT44_04250"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580044.1"
                     /translation="MIVKQQMLSNLLAPLRVRDFRLLFIGQMVSTIGDMFYAVALPWL
                     MLNSGHTPQELGIVLAAYGVPRVGTLLLGGLLSDWLRPRRVMLLADIMRAVLVAALVA
                     LVLSGRTQVLPLSVVAAPLGAFAGLFLPAYYAVLPEVLDEDALQAGNALNTSTLQLAL
                     LIGSGLAGLVVSRLSPAAAFVVDALTFVVSAFTLALMRARPQPEKALATDQSSTQESE
                     EESKRKPAFAPEITFWQLLRSWRLLQVAFLVIVFGNFLFNGLFEVALPTLARNQFMAG
                     PGGYGLLLAMFGGGSLLGGLAAGGLGQLRRRGLLLLSLIMLLGVLYSLVPFAGGLPGA
                     ALLIACAGFSNGMLTVLAFTIMQQQSPPHLLGRLLGMFLFASLGLYPLSVALVGVLSS
                     RIGPVIIFPISGAMMLLASAFGWLQRDVRQL"
     gene            4173..4523
                     /locus_tag="VGT44_04255"
     CDS             4173..4523
                     /locus_tag="VGT44_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2580045.1"
                     /translation="MKTRWAALMEPNRLHIVELLRDGPLTVGEIVERLGLPQPQVSKH
                     LKVLSETDIVEVQPQANRRIYKLRHEPFQELESWLHSFRRLWEERFDRLDDYLLDVQA
                     TEQHTSNRSEDTNV"
     gene            4516..5022
                     /locus_tag="VGT44_04260"
     CDS             4516..5022
                     /locus_tag="VGT44_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV2580046.1"
                     /translation="MFDFQSLSSRVGVQDFVVERQFTAPRALMFQVFTQPEHLKRWWA
                     PPPYTLPVCTIDLRPGGIWHYCMHSPEGQDQWARSVYREIVPPEKLVYTTTFADEHAN
                     PTLGMPEHLTTVIFTEEGGKTRVTTRVQFSSAEALKVAVDIGMPQGMTMTWAYLVQYV
                     QELRDSLS"
BASE COUNT          996 a         1526 c         1511 g         1103 t
ORIGIN      
        1 ttgcagctgg ggagtctctt gtatgagccg gtaaagcgcc tgctgggtgg agagagcaat
       61 caccaggcag taggcacaca cgaacacaac gccaatcccc caccagatag cagaccaccc
      121 atcccggcgc aaggtctgta gacccacatt gcgcgttgat agcgcacgct cagcctgact
      181 cagcctggaa gccgcgtcgt gattgccggg ttcctgctgg cctggccatg agaacgctac
      241 cccgccgata tcgcgcctgg caaagaggag cagactcgcc cccgcacaga gtacagtcag
      301 acccgccagc agcacagccg ccagaggctg gttagggaaa ctggggatga gcgggcggtt
      361 gagattgtaa tagtagaatg gtgagaggta ctgcacccaa ctcccatgca gcaagcgtcc
      421 ggtgctatcc aggagcatgg agagtactag cagtccgctc gtccagcctg ctgctacgac
      481 gcggctggcc acaaattgcg agaacagcag cgcgagactg ccaaagaaga tggccagcag
      541 gctcgcgttc agtcccgcaa gcaccgctct gaccacatcg gcgtgtccct caagacgggt
      601 ttgaccggcg agcagaccca gcgcaaccag cagcccgatc aggatcagcg cgatcacgag
      661 cgcgctcagc ttcgagagca gcaggcgtct gcgtgaccgc cctgtagcca gcaggacatt
      721 catgctgcca cgttcctctt ctccacgtac catgcgggct ccggccagga tgggccagaa
      781 gcagatggcg agtggcacga agagttccat gatacgccag gtaatgaacc cctccggggt
      841 ctggatctga tagggatcgc cgagaaaacg gaagagcggc gcgagactgc taaaggtcgc
      901 tatcgtcgtt ggcgtactct cagcgagatc gacggctgcg aacaccccca gggcgaagcc
      961 ccaggccaga atggccaccc ggtaatctct cagcgatttg ctccagatgc tgcggaacat
     1021 catgcacctc ctatccggcg agccgggcct gctcatgctc ggactcgtag aagcgcagga
     1081 agacttcttc aagcgtcggc tcgtgggtcg taatattcgt tgcgttgtgc tggctggcga
     1141 tgtgaatgat ctccgtcaag ccaccctgga cggcgagttg cagggtgcgc tcatccgccg
     1201 cctccgccac gttggctacg cccgcgacct gtttgaacca gtccacggag gctggaccgg
     1261 cgaagctgat ctccacctcg tactggtgaa tgtctttgag cgccgagaca tggtcgattt
     1321 ggatcagtcg tccctcacgg atgatcgcca cccggtcgca ggtatgctcg acctccggca
     1381 ggatatgcga ggagaggaaa acggtgcgtc cagaggcgcg caccgcggcc accaggtgat
     1441 agaattcttg ctggttgagc gggtccagcc ctgatgtggg ttcgtccagg atgagcaggc
     1501 gcggccggtg catgaaggcc tgtaccagcc ctattttctg tttgttgccg tgcgaataat
     1561 cgcggaagcg tttgctggga tccaggtcca ggcgctcgac caggctgcgc aggtagtcct
     1621 ggtccacacc gccgcgcaga tgcgccaggt actcgatgat ctgcgctccg cgcagaccgg
     1681 gatcgaaggt gaattcaccg ggcaggtagc cgaccagttg cttgacctcg gtggactgtg
     1741 cccagcaatc cagcccaccg atggtagcgc tgccactgtt cgcgtgcagc aagcccatca
     1801 ggacgcgcat cgtggtggtc ttgcccgccc cattgggacc gagaaagccg aagacttcgc
     1861 cttcttgaat agcgaaggtc acatcgagaa taccccggct cctgccgtag cttttggtaa
     1921 ggcggttggt ttgaatcatt cctgacatgc gtttcccttt tctgccagag tctccctggc
     1981 gtcatgcaaa gtcgcgtcgc cctcttcggg ctttcttcgc attgcaattc cctcgaggat
     2041 ggcatcgacg agccgttcgg gcaggtcgtg cggaagctgt atgctgtccg tgtccaaaaa
     2101 caggtgatac cacaggggac cggcgagaag accaatgatc tcgttggcgt caagatcctg
     2161 ccgcacctct ccatgcgctt gcgctcgccg aatcatctcc gcaagctgct ggaagcgagg
     2221 ggcgacgacc tggtcataga aggcctggta gacgtgagga tgagtcgtca tcacgcccag
     2281 tatgttcata gctaatcccc tcgcatcaga cccgttccag gcctggaacg cacttctggt
     2341 catgacaaga aaatcctcgc gcaacgaccc cgtgtcaatc acgggagttt gcgtctgctg
     2401 ccagcttttg atggcatcga tcaccagggc ttcttttgaa gcccaccagc gatagatcgt
     2461 ctgcttcccc accccggctt tggcggcaat cgcctcgata ctcatcgcgt caaatccttc
     2521 ggtagccagg aaggtcagag tggcatcgag aatggcttgc tgcgcttgca cactacgtgg
     2581 acgtcctcct ctagacgccc ctgattcttc ggtcgtcacg ttgatgtccc ccatctctca
     2641 gtaacattac gagaccgtct cgtttctatt gctgaagaaa caatagcacg aaaaagagca
     2701 agcgtcaagg gctggacaac tgtaggactg gatgctggac gtttcgcctg taatcctgct
     2761 atagtgagca acgtgatacc cgtctttaat cgagatgaaa ccgagttgtc ggcatgatcg
     2821 tcaaacagca aatgctgagt aatctgcttg cgccgctcag ggtgcgcgat ttccgcctgc
     2881 tcttcatcgg ccagatggtc tcgaccatcg gggatatgtt ttacgcggta gcgctgccct
     2941 ggctgatgct gaacagcggg catacgccgc aggagttggg gatcgtgctg gctgcctatg
     3001 gcgtgcctcg cgtcggcacg ctgctgctgg gcgggctgtt gagcgactgg ttgcgcccgc
     3061 gcagggtcat gctgctggcg gacatcatgc gcgccgtgct ggtcgctgcg ctggtggcgc
     3121 tggttctctc cgggcgcacg caggtcttgc cgctcagcgt cgtcgccgcg ccgctcggcg
     3181 ccttcgcggg gcttttcctg cctgcctact acgccgtgct gccggaagtt ctggatgagg
     3241 atgcattgca ggcgggtaac gccctcaata cctcaaccct gcaactggcg ctgctgatcg
     3301 gctctggcct ggcgggcctg gtagtcagcc gcctatcgcc tgccgccgcg tttgtcgtcg
     3361 atgcgctcac ctttgtcgtc tcagccttca cgctggcctt gatgcgcgcc aggccacaac
     3421 cggaaaaagc gctagcgacg gatcaatcct ccactcagga atcagaggaa gagagtaagc
     3481 gcaaacctgc attcgcgccg gaaatcacct tctggcagct tctgcgctcc tggcgcttgt
     3541 tacaggtcgc gttcctggtg attgtcttcg gcaatttttt gttcaacggc ctcttcgagg
     3601 tagctctgcc gaccctggcg cgcaaccagt tcatggcggg tccaggcggg tatgggttat
     3661 tgctggccat gtttggcggg ggttccttgc tcggcgggct tgcggcgggc ggcctgggcc
     3721 aactgcggcg gcgcggcctg ctgctgctct cgcttatcat gctactgggc gtcctctatt
     3781 ccctcgttcc cttcgcgggc ggcctgcccg gcgcggcgct gttgatcgct tgcgccggat
     3841 tctccaacgg catgttgacc gtcctggcct tcacgataat gcagcagcag tccccaccgc
     3901 acctgcttgg acggctgctc ggcatgttct tgttcgcctc gctgggcctc tatccgctgt
     3961 ctgtcgcgct cgtcggtgtg ctgtcctccc gcattggccc cgtcatcatc ttccccatca
     4021 gtggcgcgat gatgctgctc gcctcggcct tcggctggct gcagcgtgat gtcagacaat
     4081 tatagcgctt cacccaaata atctgtatgg cgaatatcct ttttaaggaa tatatcttga
     4141 caagtatatt ccttttcggg tatactatat tcatgaaaac acgatgggct gccctgatgg
     4201 agccaaaccg ccttcacatt gtcgaactgt tgcgcgatgg cccgctgacc gtaggggaaa
     4261 tcgtcgagcg gctcggatta ccgcagccgc aggtgtcgaa gcacctcaag gtgctgagcg
     4321 agactgacat tgtggaagtc cagccgcagg ctaaccggag aatttataag ttgaggcacg
     4381 aaccgttcca ggagttggag tcctggcttc actcatttcg tcgcctctgg gaagaacgtt
     4441 tcgatcgcct ggatgactat ctactggatg tacaggccac cgagcaacac accagtaacc
     4501 gaagtgagga caccaatgtt tgattttcaa tcgctaagtt ctcgtgttgg ggtacaggat
     4561 tttgtcgtgg aacggcaatt caccgcgccc cgcgcgttga tgtttcaggt gttcacccag
     4621 ccggagcatc tcaagcgctg gtgggcgcca ccaccctaca cccttcccgt ctgtaccatc
     4681 gacctgcggc ctggtggcat ctggcactac tgcatgcatt ctccggaggg tcaagatcag
     4741 tgggcgcgca gcgtgtacag ggagatcgtg cccccggaga agctggtcta caccacgacc
     4801 ttcgccgacg agcatgccaa ccccaccctt ggcatgcccg agcacctgac caccgtgatc
     4861 ttcacggaag agggtgggaa aacgagagta actactcgtg tccagttcag cagtgcagaa
     4921 gctctgaaag tcgctgttga catcggtatg ccgcaaggga tgaccatgac ctgggcttac
     4981 ctggtccagt acgtgcaaga gttgagagat tctctgtctt gaccccactt tgacccgtgt
     5041 cagtctgacg tttgtgagac tcggtgcgtg gtgttcgata aaaattgagt gcagaaggaa
     5101 aggaatgcta tgaaaaacgg agttttaact ggcgat
//
LOCUS       DASZAK010000251         1650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_503684, whole genome shotgun sequence.
ACCESSION   DASZAK010000251 DASZAK010000000
VERSION     DASZAK010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503684"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..1214
                     /locus_tag="VGT44_04265"
     CDS             15..1214
                     /locus_tag="VGT44_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580047.1"
                     /translation="MAQRTSAHSSVESKTLLEVKNLSVDYLAASGAVHAVNDVSFTLN
                     RGEILGLAGESGCGKSTLAYAIARLLRPPAYTTGGEILYYPRYREDESGKQLVSSTAL
                     SREKKKKGVEQFSPEEMDAVDILSLTNRELRSFRWSELSMVFQSAMNALNPVLKIGTQ
                     IMDVLQVHRPQMGADSRKQRAAELLQLVGIAADRINSYPHELSGGMRQRAMIAIALAL
                     NPELIIMDEPTTALDVVMQREILTEIIRLREQFDFSVIFITHDLSLLLELTDRIAIMY
                     AGRMVEMASQRDLYLHPRHPYSYGLLNSFPSLHGPRREAGGIPGSPPDLRAAPPGCAF
                     HPRCPLAFDACSKVLPLLRPSTVATPQQLVACHLYDPAISAGEPPTSQDIAAAYQAKA
                     TERNAAR"
     gene            1211..>1650
                     /locus_tag="VGT44_04270"
     CDS             1211..>1650
                     /locus_tag="VGT44_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2580048.1"
                     /translation="MSLSPQISHQSDVLDGSMPILEAEHISKVFPVRQGNPFGKRLGV
                     HAVENASIALYPGRVTALVGESGSGKTTIARMLARIYDPTSGTIRFQGQPITFQGGSA
                     LRNYRRHVQLVFQDPFASLNPVHGIRYTISRPLRVYGHAHNQAE"
BASE COUNT          323 a          590 c          442 g          295 t
ORIGIN      
        1 gaggcaatag cctgatggcc caacgcacga gcgcgcattc gagcgtagag agcaagacgc
       61 tcctcgaggt gaagaacctc tcggtcgatt acctggcggc cagcggcgct gtccacgccg
      121 tcaatgacgt cagcttcacc ctgaaccggg gcgagattct cggcctcgcc ggtgagagcg
      181 gctgcggcaa atccacgctg gcctacgcca tcgctcgcct gctgcgcccg cctgcctata
      241 ccaccggcgg cgaaatcctc tactatcccc gctatcgcga ggacgagtcg ggcaagcagc
      301 tcgtctcgtc cactgcgctg tccagggaga agaagaaaaa gggcgttgag cagttctcac
      361 cggaagaaat ggacgcggtc gatatcctca gtctcactaa cagagagctg cgctccttcc
      421 gttggagcga gctctcgatg gtcttccaga gcgccatgaa cgccctcaac ccggtgctca
      481 agattggcac acagatcatg gatgtgctgc aagtgcatcg tccccagatg ggcgctgact
      541 cccgcaaaca gcgcgccgcc gaactcctgc agcttgtggg cattgccgcc gaccgcatta
      601 acagttaccc tcatgaactt tccggcggca tgcgccagcg cgccatgatc gccattgccc
      661 tcgcgctcaa tccagaactc atcattatgg atgaaccgac caccgcgctg gatgtcgtca
      721 tgcaacgcga aatcttgacc gaaatcatcc gcctgcgcga gcagttcgac ttctccgtca
      781 tcttcattac gcacgacctt tcactgctgc tcgaactgac cgatcgcatc gccatcatgt
      841 acgccgggcg catggtcgag atggcgagcc agcgcgacct ctacctgcac ccgcgccatc
      901 cctacagcta cggcctgctc aactccttcc catcgctgca tggcccgcgt cgagaggcag
      961 gcggcatccc cggctcccca ccagacctgc gcgctgcccc gcctggctgc gcctttcatc
     1021 cgcgctgccc gctggccttc gatgcctgct cgaaggtcct gcccctcctg cgcccatcga
     1081 cggtggcaac cccgcagcaa ctggtcgcct gccatctcta cgatccagcc atcagcgccg
     1141 gagaaccacc caccagccag gatatcgcgg cagcatatca ggccaaagct acagaaagga
     1201 acgcggcgcg atgagtcttt cacctcagat tagccaccag tccgacgtac tcgacggaag
     1261 tatgcccatc ctggaagcgg aacacatctc gaaggtcttt cccgtgcgcc agggcaaccc
     1321 ctttggtaag cgcctcggcg tccacgccgt cgaaaatgcc tccattgccc tctacccagg
     1381 tcgcgtcacc gccctcgtcg gtgagagcgg cagcggcaag accaccatcg cgcgcatgtt
     1441 ggcgcgtatc tacgacccga cgagcggcac gatccgcttt cagggccagc cgattacctt
     1501 ccaggggggc agcgcgctgc gtaactaccg gcggcatgtg caactcgtct tccaggaccc
     1561 ctttgcctcg ctcaacccgg tgcatggcat ccgctacacc atcagccgcc cgctgcgcgt
     1621 ttatggccat gcccataacc aggccgagga
//
LOCUS       DASZAK010000252         4218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_433008, whole genome shotgun sequence.
ACCESSION   DASZAK010000252 DASZAK010000000
VERSION     DASZAK010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433008"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VGT44_04275"
     CDS             <1..898
                     /locus_tag="VGT44_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2580049.1"
                     /translation="AVASRDVARAQALLGHLPGVRIYGDYESLLLDPEVDAVYIPLPN
                     SLHGEWTIRALEAGKHVLCEKPLAVTAEEGHLMAETARENNLLLMEAFMYRFHPQTIW
                     ALEQAHAGRIGSIKLVRSSFSFNLMNPPRPHDIRLQAALAGGSLMDIGCYNVNFCRAV
                     YGHAPVAVAARVSAPSLDDVERSTTAVLDFGEGRFGLIDCSFELPPRQVNEIIGDAGS
                     ITIPLAFTPGHADTEVILKSEEEVLQHHISGIDQYRLEVEHFGDCIRVGAHPAFRLSE
                     TLENLRTMEAIYTSAGHRWPLY"
     gene            complement(895..1818)
                     /locus_tag="VGT44_04280"
     CDS             complement(895..1818)
                     /locus_tag="VGT44_04280"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HEV2580050.1"
                     /translation="MSKLQLFLQVTRARTLPVMISPVVIGAILAWQQGYVFSWSLFAL
                     TLVGALAAHLGANVLNDVFDFGSGADQAAQEITPQGQTAVTGSRFLLSKQVSMSSYRW
                     LAVACFAVALLCGIILSIFRPLALVFGAAGLLLAFFYVAPPLRLAYIGRGLGEVDILI
                     SFGMLPLVGAFYVQSCVVTLTALLASLPIGLYTMSVLYFHHFLHWRGDQAAGKITPVV
                     ALGEGGARVAGMLILLLIALAFIVDVVLSVYPWYSVVAILTVLPTQFRLQRATGDLKY
                     YLQLMATNFNGNLQAALLVTLALLIRGFVRI"
     gene            complement(2030..2881)
                     /locus_tag="VGT44_04285"
     CDS             complement(2030..2881)
                     /locus_tag="VGT44_04285"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV2580051.1"
                     /translation="MAENKGYVHPEVLVDAEWVEVHLHDPRVRLIEVDVDTSAYEQGH
                     IPGAVGFNWQKELQDQVIRAPLSQAHLEELLSSAGVSNDTTIVLYGDNNNWFAAWAFW
                     IFKYYGHQDVRLLDGGRVKWLADKREITTDAPSYARTTYHAQAPVHHIRALRDQIFTQ
                     LGQQGFALVDVRSPGEYSGELLAPAHLPQEGAQRGGHIPGAANIPWSMAVREDGTFKS
                     AGELRALYEGKGVTPDKNVVAYCRIGERSSHTWYVLNYLLGYPHVRNYDGSWTEWGSL
                     IGAPIEK"
     gene            3107..3997
                     /locus_tag="VGT44_04290"
     CDS             3107..3997
                     /locus_tag="VGT44_04290"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV2580052.1"
                     /translation="MNLYHLRVFMAVAEHEHITRASGELYLSQPAVTKIIQHLEQETG
                     QELIERHGRRIVLTDAGKLLHEYARRIFALEREMEDALAALRDAGVGKIHLAANTTMG
                     VYLLPPVVAKFRELHPKVELWITILNSHEIIEDILNWEIDFGLVEGDPSTLPPGLSAE
                     VFTQDELVLVVAPTHHWSSRKALVPGALGEGVLLLRERGSGIRETIEHVFLHQGVQIQ
                     PLLTLPDNEAIKQMVMNGVGAAFVSELAVRRELANGELVRIPISGVELRPQLSLVRRA
                     DKHLSRAADAFCALLRGDVA"
BASE COUNT          900 a         1249 c         1222 g          847 t
ORIGIN      
        1 ggccgtcgcc agccgcgatg tggctcgcgc gcaggcgctg ctggggcatc taccaggtgt
       61 gcgcatctac ggcgattacg agagcctgct gctcgacccg gaggtcgatg cggtctacat
      121 tcccctgccg aatagtctgc atggcgaatg gaccatccgc gcgctggagg ccggaaagca
      181 tgtcctctgc gagaagccgc tggcggtcac ggcggaagaa ggccacctga tggccgaaac
      241 cgcgcgcgaa aacaacctgc tgctgatgga agccttcatg taccgttttc atccgcagac
      301 catctgggca ttagagcagg cgcatgccgg gcgcatcggt tctatcaagc tggtgcgctc
      361 ctcgttctcg ttcaatctca tgaatccgcc gcgcccgcac gatattcgcc tgcaggcagc
      421 cctggccggc ggctcgctga tggatatcgg ctgctacaac gtcaactttt gccgcgccgt
      481 ttatggacat gcgccagtcg ccgttgccgc gcgcgtctcg gctcccagcc tggatgatgt
      541 agaacgatcg accaccgccg tactcgattt tggcgaaggc cgcttcggac tgatcgattg
      601 cagctttgaa ctgccccctc gccaggtcaa tgagatcatc ggcgacgcgg gcagtatcac
      661 cattccgctg gcctttacac ccggtcacgc cgataccgag gtgatcctga agagcgaaga
      721 ggaggtatta cagcatcaca tcagcggcat agaccagtat cggctcgaag tcgaacattt
      781 cggcgattgt atccgcgtcg gcgcgcaccc tgccttccgc ctgtcggaaa ccctcgaaaa
      841 tctgcgcacg atggaggcga tctatacatc agccgggcat cgttggccat tatattaaat
      901 gcggacaaat ccgcgaatta gcagcgccag cgttaccagc agcgcggctt gcaggttgcc
      961 gttaaagttg gtcgccatca gttgcaggta gtatttcagg tcgccggtgg cgcgctgcag
     1021 gcgaaattgt gtggggagga cggtcaaaat ggcgaccacg ctgtaccagg gatagacgga
     1081 taagaccaca tcgacgatga acgccagcgc gatgagcagc aggatcagca tcccggccac
     1141 gcgcgcgcct ccttcgccca gcgccaccac cggtgtgatc ttgcccgccg cttgatcgcc
     1201 gcgccagtgc aggaaatggt ggaagtacaa caccgacatc gtgtagagtc cgatgggcag
     1261 cgaagccagc agcgcggtga gcgtcaccac gcacgattgc acgtaaaacg ccccgaccag
     1321 gggcagcatt ccgaacgaga tcaaaatatc cacttcgccc aggccgcgcc cgatgtaggc
     1381 cagtcgcagg ggaggagcga cgtaaaagaa cgccagcagc aagcctgctg cgccgaagac
     1441 cagcgccagg ggccggaaga tgctcaggat gatgccgcag agcagcgcga cggcgaagca
     1501 agccaccgcc agccagcgat acgacgacat gctgacctgc ttgctgagca ggaaacgcga
     1561 gccggtcaca gcagtttgcc cctgcggcgt gatctcctgc gcggcctggt ccgcgcccga
     1621 tccaaaatca aagacatcgt tcagcacgtt cgcccccagg tgcgccgcca gcgcgcccac
     1681 cagcgtcagc gcgaataggc tccaggagaa gacataccct tgctgccacg ccaggatcgc
     1741 tccgatcact accggcgata tcatgaccgg caaagtgcgc gcgcgcgtca cttgcaggaa
     1801 taattgcagc ttgctcatgc ttcctcacct ggcgctaccg gctcaaacga cgccccggta
     1861 tatacagtgt tctcgtgtca tgatctgtat tttagcacga attgagcaag atacaggttg
     1921 atgaaaatcg agaaacaagc gtgagaggca tggcgaccct tgcggtcgcc atgcctctca
     1981 cgacatgtca tcagccggta tcttgctcaa aactctcaaa tgggatggct tatttctcga
     2041 ttggcgcgcc gatcaggctg ccccactcgg tccacgagcc gtcgtagttg cgcacgtgag
     2101 ggtagccgag caggtagttc aggacatacc aggtgtgact gctgcgctcg ccgatgcggc
     2161 aataggccac cacgttctta tccggggtga cgcctttacc ctcgtatagc gctcgcagtt
     2221 caccggctga cttgaacgtg ccgtcctcgc gtaccgccat tgaccagggg atgttggccg
     2281 cgccagggat atgtccgccg cgctgcgcgc cttcctgcgg caagtgtgct ggcgccagca
     2341 gctcaccgct atattcgccg ggcgagcgca catcgaccag cgcaaaacct tgctggccca
     2401 actgtgtgaa gatctggtcg cgcaacgcac gaatgtggtg tacgggcgcc tgcgcgtggt
     2461 acgtggtacg cgcataggat ggcgcgtcgg tcgtgatctc gcgcttatcg gcgagccact
     2521 tgactcgccc gccgtctagc aaacgcacat cctggtgtcc ataatattta aagatccaaa
     2581 aagcccaggc tgcgaaccag ttgttgttgt caccatacaa cacaatcgtg gtatcgttgc
     2641 tcacgcctgc gctgctgagc aattcttcca ggtgcgcctg gctcaacggg gcgcggatga
     2701 cctggtcctg tagctccttc tgccagttaa acccgaccgc gccgggaatg tgtccttgct
     2761 cgtaggcgga tgtatcgaca tccacctcga tcagccgtac cctgggatcg tgcaggtgca
     2821 cctcaaccca ctctgcatcg accagtacct ctggatgaac gtaccccttg ttttcagcca
     2881 tgattactcc ttacttgaaa tagacgaaac tggtcacgcg tataacacgg ctacattctt
     2941 gacaaagtag ctctactaac taaacaacat gatatgctaa aatttaattc ccctgcaagc
     3001 cgtcctatgc aatttgatta tgcaggttat tccagcagat tatacaatgt gaggcgtgtg
     3061 gatgtgatac gctatcgatc agggataggg caaaaaggag acggttgtga acctgtatca
     3121 tttacgtgta tttatggctg ttgctgagca tgaacatatt acgcgagctt ctggagaatt
     3181 gtatctgagc cagccggctg ttacgaaaat catccaacat ctcgaacagg aaaccggcca
     3241 ggagctgatc gagcgtcacg gcagacgcat cgttctgacc gatgctggta agttgttgca
     3301 cgagtatgcg cggcgcatat ttgccctgga acgcgaaatg gaggatgccc tggcggcgct
     3361 gcgcgatgca ggagtaggca aaatccacct cgcggccaat accaccatgg gcgtctattt
     3421 gctgccgccg gtcgtggcga agttccgaga actccatccg aaagtggagt tatggatcac
     3481 gatcctcaac tcgcacgaga tcatcgaaga tattctaaat tgggagatcg atttcgggct
     3541 ggtcgagggc gacccgtcta cgctgccacc gggattatcg gcggaggtct tcacgcagga
     3601 cgagctggtg ctggttgtcg cgccaacgca ccattggagc agccgcaaag cactggttcc
     3661 aggcgcgctg ggcgagggcg tcttattgct gcgcgagagg ggcagcggta ttcgagaaac
     3721 catcgagcat gtcttcctgc accagggtgt gcagatacaa cccctgttga ccctgccgga
     3781 taacgaggct atcaagcaga tggtcatgaa cggcgttggc gcggccttcg tctccgagct
     3841 ggccgtgagg cgcgaactgg caaacggcga gctggtgcgc atccccatca gcggcgtcga
     3901 actgcggccc cagctcagcc tggtgcggcg cgccgacaag catttgtcac gggcggcgga
     3961 tgccttttgc gccctgcttc gcggcgatgt cgcttgactt tcctactgca ttggagtacc
     4021 atagccacgc ccggtaactc atgttaccag gcacaccaca attgtttggg agcggctatt
     4081 catgcatgaa cgggaagcga tgcgctggtt gcagttaacc tacaaggtac ccagcgagcc
     4141 ttcgcagaag cgcgtctggg tctggcggcg gttacaaaat ctgggagcgt tcgccctaca
     4201 aaattccgtc tacctgct
//
LOCUS       DASZAK010000253        11690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_681262, whole genome shotgun sequence.
ACCESSION   DASZAK010000253 DASZAK010000000
VERSION     DASZAK010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11690
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681262"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="VGT44_04295"
     CDS             <1..1159
                     /locus_tag="VGT44_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580053.1"
                     /translation="LSRRKVLALAGSGTFVLVAGGGVWRAADQGVFSTGEGPAYEPWD
                     DWRTATRGPLDLVRAAILAANPHNSQPWLFHVTRTQIDLYADLRRNLGSIDPFLREMH
                     LGLGCALENLELAAPANGYATQITLMPDALDETFVARIDLAAGSAPVSELYSLIPQRH
                     TNRYPYDTGRPVVTTTLDALSALNNDPAVQVFWFASEPMRKRIGTLLVDAAHALVADN
                     TQDGDSNSWYRATWQDLQQHRDGITLDAAGLPDWERALGKILPPPSQEQQDSTFLSNT
                     AEQAQTAGAFGLLTVRNAQDRTQQMRAGRLWERLQLWATKEGLAMQPLNAVVERAARE
                     VVLGSTPHFGAALAALVGDPAWQTLLAFRIGYSTYDGLRSPRRGVDEVVNP"
     gene            1179..1901
                     /locus_tag="VGT44_04300"
     CDS             1179..1901
                     /locus_tag="VGT44_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580054.1"
                     /translation="MPITWWSLLIWLFALVLGAFLVTWVFADLLRLTQTVYIGVLAVV
                     TGVFLYGYLSWSNIDWGAFLSHQWVWGLVGAVVAGPVLIMLLAQGARRSHSRVLAPTP
                     RPEGLRLAGALLWEGLVYGTAEGLLLSVLPVLVTWQAVSALGWTQSWMGVVSAGVVAI
                     VVSLLVIVVHHLGYREFRGPQLAGAMMTCGLLSLAYLLTMNPLAAAFGHSIAHSGAIL
                     HGTELPPHQESGHSTASKVLAH"
     gene            complement(2152..4497)
                     /locus_tag="VGT44_04305"
     CDS             complement(2152..4497)
                     /locus_tag="VGT44_04305"
                     /EC_number="7.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasma-membrane proton-efflux P-type ATPase"
                     /protein_id="HEV2580055.1"
                     /translation="MTISHHELAGLTTDEAKARLAQYGSNEVSAAKPNALLTLLRKFW
                     APVPWMLEATIALTLLSARYLDAAVIGFLLVFNIIISFFQEGRAEHTLALLRERLAIN
                     ARALRDGQWRQVAARELVPGDVIHVRMGDVIPADTTVHDGALVVDQSPLTGESVPVEK
                     GVGETLYAGAIARQGEATGTVIATGAQTSFGKTTELVKTARTVSHLETVILHIVTYLV
                     VIDVLLVMAMLAFATVVHISLLDALPFALIVLVASVPVALPATFTLAQALGAQDLAKR
                     GVLVTRLSAIEEAASMDVLCTDKTGTLTLNQLSVGQIKSSPPFSADELLTYGAIASDA
                     ASQDPLDLAILAAHQAQHLAELPERLSFTPFDPASKRTEASIQRDGARIIVTKGAPQM
                     VAALVTGSDQEALAGEVAVLAAQGYRVLAVAAGPEQALALVGLIGLSDPPRPDSAALI
                     ASLHTLGVQTKMLTGDTVETARAIAKEVGIAGLVCDAAALKAQGTHFDRECAVLAGIF
                     PEDKFHLVQALQAGRHIVGMTGDGVNDAPALKQAEVGIAVSSATDVAKAAASIVLTTP
                     GLLDILAAVETSRRVYQRMHTYTLNKIIKTIQVAAFLIVAFFITRHFVITPTLIVLLL
                     FANDFVTMSLAADRARPTPAPDRWRVGHLVGSALALAAVLLVESFLLLWLMPGVFHQS
                     LAETQTLVFLMLVFSGQTTVYVVREQRHVWSSLPGRTLLIASACDLLVVSGLAALGIL
                     MTPVSPLLIGVTFALALVFMLLLDQVKQWVVRRPALPSRAQ"
     gene            complement(4637..5428)
                     /locus_tag="VGT44_04310"
     CDS             complement(4637..5428)
                     /locus_tag="VGT44_04310"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV2580056.1"
                     /translation="MAVNRLNAGPVPLYHQLKQVLREQIERGDYRPGDRLPSEPELIR
                     QYGISRITVRQALDELEAEGLIVRRHGKGTYVAERRIEHELVRLTDFMEDMQQASQHP
                     SSRVLAFVHEPASPPIASALHLKEKTEVVRVDRLRLADERPMACDTTWLPLRFGTLLV
                     GIDLTQETIYHLLETRYAIPVVSGTFSITATTATNHQADLLEMPAGDALLLIQRISYT
                     TGGEPVYVQDRYYRPDRVQYRVMLRRHNNEAGSPKIDEFRAVFAE"
     gene            5633..6424
                     /locus_tag="VGT44_04315"
     CDS             5633..6424
                     /locus_tag="VGT44_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV2580057.1"
                     /translation="MGSLTLLQQVLAVFCGALVGFTLGLIGGGGSILAVPLLLYVVGY
                     HNPHVVIGTTALAVALTAYINLIPHWRIGNVRWLPAVAFAIPGVVGAALGAQIGKIVP
                     GKQLLFLFALLMIGVAITMLRPAKSGTPKPVYAGKPMIARTTPVGFLVGTLSGFFGIG
                     GGFLIVPGLMFAAGMPLLNAIGTSLFSVGMFGTTTAITYALSGLINWAIALEYIAGGV
                     LGGLAGARLATHLGKQKKTLSRIFAGVILVVAVYMLYVNFTAIRL"
     gene            complement(6446..6787)
                     /locus_tag="VGT44_04320"
     CDS             complement(6446..6787)
                     /locus_tag="VGT44_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2580058.1"
                     /translation="MVFQHLFHRSSVEEVSAEEAKAKQKAGAVIIDVREPHEWREGYI
                     PGAKLIPLGSLAKRAQELDSSKEIIAVCRSGNRSISAALILQRAGFTQASSMAGGMIS
                     WIRHRYPVSRA"
     gene            complement(6882..7079)
                     /locus_tag="VGT44_04325"
     CDS             complement(6882..7079)
                     /locus_tag="VGT44_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2580059.1"
                     /translation="MPIYVYRCERCKERFERLVPMSANSANASCPNCGAADARKQIST
                     FAAPQKGASTGQSSCAPTGGG"
     gene            complement(7082..7429)
                     /locus_tag="VGT44_04330"
     CDS             complement(7082..7429)
                     /locus_tag="VGT44_04330"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2580060.1"
                     /translation="MEREQEQQLYRLQADLCQTLADPTRLEMLTLLGEGPKAVKDLVA
                     LLGQRQAKVSQHLALLRQRGIVRAERAGNEMHYSLADVRILDACHITREILLQQLTQQ
                     GTLATMLAGTRER"
     gene            complement(7612..7956)
                     /locus_tag="VGT44_04335"
     CDS             complement(7612..7956)
                     /locus_tag="VGT44_04335"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2580061.1"
                     /translation="MEKEFYRRHAQLCGVFAHPLRLELLQLLHDREECTVGDLAEATG
                     VRMANLSQHLAIMRSTNVVVTRKEGVSVYYRIADPRIMQAFDLITDVMLGQMAREGEL
                     RREFAANLQDSE"
     gene            complement(8138..8344)
                     /locus_tag="VGT44_04340"
     CDS             complement(8138..8344)
                     /locus_tag="VGT44_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580062.1"
                     /translation="MSMELDVVIDSCVKMLQSDPQRTREEIAQLLNRWEELGYISRQM
                     RAVIVQRLRQNQPGEQAPGKTSEL"
     gene            complement(8341..9126)
                     /locus_tag="VGT44_04345"
     CDS             complement(8341..9126)
                     /locus_tag="VGT44_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV2580063.1"
                     /translation="MPNERACLSALRGVSARAAAILDHYTNEQALWVPVGAFQQFAHT
                     LHWGRFEDLDLCLLDGLDVDDRGALLEELRAYNAALQRQRDCVDRFHRAYRQWIDQQH
                     EALPSSYRERLRKWQRRYGRYDYYRVTGLGTQSGIERFLADPEENMRAFEQAFLDLEE
                     QRQRERARHKQAEADWWANLDGLPGNGYALSQLDEALHLLGLSPGATLAEIQRAYRAY
                     AKLVHPDRQGAGSTERMAALNRAYAYLRKLYRSAEPEMRRAEA"
     gene            complement(9183..9761)
                     /locus_tag="VGT44_04350"
     CDS             complement(9183..9761)
                     /locus_tag="VGT44_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEV2580064.1"
                     /translation="MDMQTESQAPGLSSDIARLVREQVDTYLQEKEAEKETRTPRLAM
                     IATKGTLDWAYPPLILATTAAALGWDVGIFFTFYGLNIIHKEKSKHLRVAPLANPAMP
                     MPVPNIVGAIPGMTAMGTIVMKGMFKSHHVSTIDELMEMAIESGVKLWPCGMTMDVFG
                     YKAEDFIDGIQEVCGATHFLGFAKDADVSLFI"
     gene            complement(9881..10339)
                     /locus_tag="VGT44_04355"
     CDS             complement(9881..10339)
                     /locus_tag="VGT44_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580065.1"
                     /translation="MTAHIYRGCEFPADCFYDVERDIWVRRDEDGCVTMGMTDPAQAQ
                     CGKFVHVRFKAVGRTLQRGQSAATIESAKWVGPFPTPLSGEIVATNETEFKRDILLAN
                     KDPYGAGWLIRLRPAKTEEEWPSLLPHDQAFERYKEKIVERDVQCYRCAD"
     gene            complement(10364..10801)
                     /gene="gcvH"
                     /locus_tag="VGT44_04360"
     CDS             complement(10364..10801)
                     /gene="gcvH"
                     /locus_tag="VGT44_04360"
                     /inference="COORDINATES: protein motif:HMM:NF002270.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEV2580066.1"
                     /translation="MGTANGCNIPEDLYYLVDKHVWARREPDALVTVGITDVAQHLAG
                     KVIAVSPKKVGRALAKGQSAATVESGKWVGPVPAPVGGELVAVNDALKGNPALINQDP
                     YGAGWIVKLKPLDWDEESKDLVTGDQGVAVYQQALDDKGIACQ"
     gene            complement(10840..11109)
                     /locus_tag="VGT44_04365"
     CDS             complement(10840..11109)
                     /locus_tag="VGT44_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580067.1"
                     /translation="MRCPYCDVEGSRQVIHAHLTDQHGDKVIARRDEQTGRLFYRLDC
                     PQCAEYSEREVNPRGRDPTFLQAFEREIRLVAFDLLLYHIEDMHT"
     gene            complement(11112..>11690)
                     /locus_tag="VGT44_04370"
     CDS             complement(11112..>11690)
                     /locus_tag="VGT44_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV2580068.1"
                     /translation="MKSLGKAAFKTIVTTDPHSLNALRNEYGQLGGNYEVIHYTELLC
                     RLIESGQLRLERPLSYHVTYHDPCYLGRYNRVFDAPRNILKTLGLQVTEMGRCRENSF
                     CCGAGGGRIWMTETNIQERPSVNRIREAVALGNVDYFVVACPKDVTMYTDAVKTSGYE
                     GQIVVKDIIELVHEALQTQSAVAASSQTAEGL"
BASE COUNT         2200 a         3525 c         3509 g         2456 t
ORIGIN      
        1 gctcagtcgc agaaaggtcc tcgcgctcgc cggcagcggg acgttcgtcc tggtcgccgg
       61 cggaggggtc tggcgcgccg ccgaccaggg ggtgttcagc accggtgaag ggcccgcgta
      121 cgagccgtgg gacgactggc gtaccgcgac caggggaccg ctcgacctcg ttcgtgcagc
      181 gatcctggca gccaatccgc ataacagtca accctggctc ttccatgtga cccggacgca
      241 gatcgacctg tacgccgacc tcaggcgcaa tctcgggagc atcgatccct tcttacgcga
      301 gatgcacctc ggcctgggct gcgccctgga aaacctggag cttgcagcgc cagccaatgg
      361 ctatgctact cagatcacgc tgatgccgga tgcgctggat gagacgttcg tcgcccgtat
      421 cgatctcgca gcagggagtg cgcctgtctc tgaactctac tctctgatcc cgcagcggca
      481 caccaatcgc tacccctatg acacgggccg tccggtggtc accacaaccc tggatgctct
      541 gagcgccctg aacaacgatc ccgctgtcca ggtcttctgg ttcgccagcg agccgatgcg
      601 caagaggatc ggtaccctcc tggtggacgc agcgcatgcg ctggtggccg acaacaccca
      661 ggatggggat agcaacagtt ggtatcgggc cacctggcaa gacctccagc agcaccgcga
      721 cggcatcacc cttgatgcgg cggggctccc cgattgggaa cgcgctctcg gcaagatact
      781 gcccccgcca tcgcaggagc agcaggattc gacgttcctc tcgaacactg ccgagcaggc
      841 gcagacagca ggagcctttg gcctgctcac agtacgcaat gctcaggaca ggacgcaaca
      901 gatgcgtgca ggccgcctgt gggagcgctt gcagttgtgg gccacgaagg aggggctagc
      961 catgcagccg ctcaacgcgg tcgtggagcg tgccgccaga gaagttgtcc tggggagcac
     1021 gccgcacttt ggggcagcgc tcgcagccct ggtgggtgat cctgcctggc agacgctttt
     1081 ggccttccgc attggctact cgacctacga cgggctgagg agtccacgcc gaggggtgga
     1141 cgaggtggtg aacccatgaa cagaaagaag tgattcaaat gccaattacc tggtggagtc
     1201 tgttgatctg gttgtttgcg ctcgtactcg gagcgtttct ggtgacgtgg gtctttgctg
     1261 atctgcttcg cttgacacaa acagtataca tcggcgtact ggccgtcgtg accggcgtgt
     1321 ttctttatgg atacctcagt tggagcaaca tagattgggg agcgttcctg agccatcagt
     1381 gggtctgggg cctggttgga gcggtagtcg ctggacccgt gctgatcatg ctcttagctc
     1441 aaggggcgag gcgttcacac tcgcgcgtac tcgcaccaac gccaagaccg gagggactgc
     1501 gtctcgcggg cgctctcttg tgggagggtc tcgtgtacgg cacggcagag gggctcttgc
     1561 tttcggtcct tcccgtcctt gtgacctggc aagcagtgtc agcgctcgga tggacacaga
     1621 gctggatggg tgtggtgtcc gccggtgtgg tcgcaatcgt cgtgagtctg ctggtcattg
     1681 tggtgcatca cctgggctat cgtgaatttc gcggaccaca actcgcagga gccatgatga
     1741 cctgcggtct cctcagcctg gcctacctgc tgacgatgaa tccactcgct gctgctttcg
     1801 gccacagcat tgcacatagt ggagccatac ttcatggtac ggagttgccg ccgcatcagg
     1861 agagcggcca cagcacagca agtaaggtcc ttgctcacta atgccaacaa gaggactacc
     1921 tgctccgcct gtctgcgcgt ctccggtcgc aaggactcga cggccatcat cctgccatca
     1981 tctggtcgct gcatgtctcc gaggagatca gcgccgccct catccacgcg ggagagcaag
     2041 cggatcagga gggacacgag gcgctgttcc tggcactggc aacgcacggg cgcggttggt
     2101 ggagtggcag catggctgtg agcctccttg aacacagcat gctccctttg ctcattgtgc
     2161 gcgcgagggg agagcgggac ggcgcacaac ccactgcttg acctggtcga gcagcagcat
     2221 gaacacgagt gccagggcaa aggtgacgcc gatcaagagt gggctgacag gggtcatcag
     2281 gatgcccagt gcggccagcc ccgagacgac gagcagatcg caggcggagg caatcagcag
     2341 cgtcctgccg ggcagcgacg accagacgtg acgctgctcg cgcacaacgt acaccgtcgt
     2401 ctggccggag aagaccagca tgaggaacac cagcgtctgc gtctcggcca gtgactggtg
     2461 aaagacgccc ggcatgagcc acaataacag gaatgactca accagcagaa cggcagcgag
     2521 cgcgagagcc gaaccaacca ggtgtcccac ccgccagcgg tccggcgcgg gagtgggccg
     2581 cgcgcggtcg gcggcaagcg acatggtgac gaagtcgttg gcgaagagca gcagcacgat
     2641 cagggtgggc gtgatgacga agtgacgggt gatgaagaac gcgacaatga gaaaggccgc
     2701 gacctggatg gtcttgataa tcttgttgag tgtgtaggtg tgcatgcgct ggtagacgcg
     2761 gcggcttgtc tcgacggcgg ccaggatgtc gagcaggccg ggggtggtca gcacgatgct
     2821 ggcggcggct ttggccacgt cggtcgcgga ggagacggcg atgcccacct cggcctgctt
     2881 gagcgcgggc gcgtcgttca cgccgtcgcc ggtcatgccc acgatatgcc gcccggcttg
     2941 cagggcctgg acgagatgga atttgtcctc ggggaagatg cccgccagga cagcgcactc
     3001 ccgatcgaag tgcgtcccct gggccttgag cgcggcggca tcacagacca ggccagcgat
     3061 gccgacctct ttcgcgatgg cacgcgccgt ctcgaccgta tcaccggtga gcatcttcgt
     3121 ctggacaccc agggtgtgca ggctggcgat cagcgcggcg gagtccgggc gcggcggatc
     3181 ggacagcccg attagaccga ccagggctag cgcctgttcc ggcccggctg caaccgccag
     3241 cacgcgatac ccctgggccg ccaggacggc tacctcccct gccagcgcct cctgatcgct
     3301 gccagtcaca agcgcagcga ccatctgcgg cgcgcctttg gtcacgatga tccttgctcc
     3361 gtcgcgttgg atgctcgcct cggtgcgttt ggaggcggga tcgaaaggtg tgaagctgag
     3421 gcgttcgggt agttcggcaa gatgctgtgc ctggtgcgcc gcgaggatgg caagatcgag
     3481 cgggtcctgg ctggccgcat ccgaggcgat ggctccgtag gtcagcagct cgtcggcgct
     3541 gaacggcggg gaagatttga tctgcccgac gctgagctgg ttgagcgtga gcgtcccggt
     3601 tttgtcggtg cagagcacgt ccatgctggc agcctcctca atggcagaga ggcgcgtgac
     3661 gagcacgccg cgtttcgcca ggtcctgcgc acccagcgcc tgtgccagcg taaaggtcgc
     3721 ggggagcgcc acggggacgg aggcgaccag cacaatcaac gcgaagggca gcgcatccag
     3781 cagagagata tggacaacag tggcgaaggc cagcatcgcc atgacgagca ggacatcgat
     3841 caccacgaga taggtgacga tgtgcaggat gaccgtttcc aggtgcgaga cggtgcgcgc
     3901 cgttttgacc agttcggtgg ttttgccgaa ggaggtctgc gcgccggtgg cgataaccgt
     3961 gccggtcgcc tcgccctgcc gggcaatcgc gccagcgtac agtgtctcgc ccaccccctt
     4021 ctcgaccgga accgattcgc cggtgagcgg ggattgatcg acgacgagcg ctccatcgtg
     4081 aaccgtagta tcggcaggga tgacatcgcc catgcgcacg tggatcacat cccccggcac
     4141 cagctcgcgc gccgccacct gccgccactg tccatcgcgc agcgcgcgcg cattgatcgc
     4201 caggcgctcg cggagcagcg ccagggtatg ctcagcccgc ccttcctgga agaaactgat
     4261 gatgatattg aagaccagca gaaagccgat gacggcagca tcgagatagc gcgccgagag
     4321 cagcgtaagc gcgatggtcg cttcgagcat ccaggggacg ggggcccaga atttgcgcag
     4381 cagcgtgagc agcgcgttcg gcttcgctgc gctgacttcg ttggagccgt actgcgccag
     4441 tcgcgctttc gcttcgtccg ttgtgagtcc ggcgagttcg tgatgcgaga tggtcatacg
     4501 aatagtacct ctcatcgtgg gatgagcaaa catgtgcctt tagcatacca taggttgacg
     4561 gtcacggttc tctcgcacga aatcaggaag aaggccattc agcccgggtt tcggtatcat
     4621 ctccttccgc tggtgcttac tcggcaaaca ccgcacggaa ctcgtcaatt ttcgggcttc
     4681 ctgcttcgtt gttgtgccgc cgcagcatga cacgatattg cacgcggtca ggtcgatagt
     4741 agcggtcctg gacgtacacc ggttcgcctc cggtcgtata tgagatgcgc tgaataagca
     4801 gaagggcgtc tccagcaggc atttccagca ggtcggcctg atggtttgtc gcggtggtgg
     4861 ccgtgatcga gaaggtgccg gagaccaccg gaatggcata gcgcgtctcc aggaggtggt
     4921 agatggtttc ctgagtaaga tctatgccta ccagcaatgt gccaaaacgc aggggcagcc
     4981 aggtggtatc gcacgccatt ggccgttcat cggccagccg cagccgatcc acgcgcacca
     5041 cctctgtctt ttctttcagg tgcagcgcac tggcgatcgg cgggctggct ggttcgtgta
     5101 cgaatgccag gacgcgcgag gagggatgct ggctcgcctg ctgcatgtcc tccatgaagt
     5161 cggtcagccg aaccagctcg tgttcgatac gccgctcggc cacgtaggtt cccttgccgt
     5221 ggcggcgcac gatcaacccc tcggcctcca actcgtcaag cgcctggcgc acggtgatgc
     5281 ggctgatccc gtactggcga atgagttctg gctccgaggg caggcgatcc cctggacgat
     5341 agtccccacg ctcgatctgc tctcgcagca cctgtttcaa ctggtgatat aaaggcacgg
     5401 gaccggcatt cagacggttt acagccatga gatatctcct tggtgcagtt gttcatccaa
     5461 tatcgtcatg ccaccatcac aatcgtatca catcgtcaga ctcatcataa cttgttatat
     5521 cgtcatgttg acattggctg acataccata ttatactcta attgatgctt tcaaaacaac
     5581 atcgcaagag tgagatcgga ggagaagcaa cattgaacaa gggacgcatg tgatgggatc
     5641 attgactctg cttcagcagg tactggccgt tttctgcggt gcgctggtgg gctttaccct
     5701 gggattgatc gggggcggcg ggtcgattct cgcggtaccg ctgctcctct atgtagtagg
     5761 gtatcataat ccgcacgtgg taatcggaac aacggcgctg gctgtggccc tgaccgccta
     5821 catcaacctg atcccgcact ggaggatcgg caatgtgcgc tggctgcccg cggtggcctt
     5881 cgccataccg ggcgtggtgg gcgcagcgct tggcgcgcaa atcggcaaaa tcgtgcccgg
     5941 caagcagttg ctctttctct tcgccctgtt gatgatcggt gttgcgatta ccatgctgcg
     6001 cccggcgaag tccggcactc ccaaacctgt ctatgctggc aagccgatga tcgccaggac
     6061 cacgccggtc ggcttcctgg tcggcacgct ctcgggcttc tttggcattg gaggagggtt
     6121 cctgattgta cccggcctga tgtttgccgc aggcatgccg ctcttgaatg cgattggcac
     6181 ttcgctgttc tcggtcggca tgttcggcac gacaaccgcg attacgtatg ccctctccgg
     6241 cctcatcaac tgggctatcg cgctcgaata tatcgcgggc ggcgtcctgg gcgggctggc
     6301 cggggcgcgg ctggcaacac acctgggcaa gcagaagaaa acgctttccc gtattttcgc
     6361 cggggtgatc ctggtggtcg cggtctatat gctctacgtc aattttacag ccatacgcct
     6421 gtgagttacg aacgcaaacg cctgattatg ctcgactcac cgggtagcga tgccttatcc
     6481 agcttatcat acctcctgcc atgctgctcg cctgtgtgaa gccggctcgc tgcaagatca
     6541 acgcggcgct gatactgcgg ttcccgctgc ggcagacagc gatgatctct tttgatgagt
     6601 ccagttcctg cgcgcgtttg gcgaggctgc ccagaggaat gagcttcgct ccaggaatat
     6661 atccctcgcg ccattcgtgc ggttcacgca catcgatgat caccgccccg gctttttgtt
     6721 tcgctttcgc ctcctccgct gacacttcct ctaccgaaga cctgtgaaac aagtgctgaa
     6781 aaaccatact aaaactcctg tgttgtttgt tctttggaga tcctggtcag aaccctcaca
     6841 cgctctatcc agttggagcg accgctcctc tagcggatgc ttcagccacc gccggtcggg
     6901 gcgcaggagg actggccggt tgacgcccct ttctgtggag ccgcaaaggt cgagatctgc
     6961 ttgcgcgcat ctgctgcgcc gcagtttggg caactggcgt tagcgctgtt ggcgctcatc
     7021 gggaccagtc gctcaaagcg ttccttgcag cgttcgcacc gatacacata gataggcata
     7081 ctcagcgctc cctcgttcca gccagcatgg tggccagcgt tccctgctgc gtgagttgct
     7141 ggagcaaaat ctcgcgcgtg atatggcagg catccagaat acgcacgtct gccagcgaat
     7201 aatgcatctc gttgcccgcg cgctcagcgc gcacaatgcc gcgctggcgc agcagggcca
     7261 ggtgctggct cactttggcc tgtcgctggc cgagcagtgc gaccaggtct ttgacagctt
     7321 tcggcccctc gccgagcagg gtaagcattt ccagccgggt cgggtcggcc agcgtctgac
     7381 agaggtcggc ctgcaaacgg tacagctgct gttcttgctc tcgttccatt tttttcatcc
     7441 tgacataata ttcagatcat cgaataatcg aatgttatca tagggaaaag caaatgtcaa
     7501 gggacataac cagttttctt cactgttctg gtgatgaggc agcagtctat ggggaggttc
     7561 ctggcgcgaa gcagctaact tgaagacggt ctgcgtacat ttgtgtcgct tctactccga
     7621 gtcctgaaga ttggcggcga actcgcggcg tagttccccc tcgcgggcca tctggcccag
     7681 catcacatcg gtgattagat cgaatgcctg cataatgcgg gggtcggcaa tacggtaata
     7741 gacgctgacg ccctctttgc gcgtgaccac aacattcgtt gaacgcatga tagcgagatg
     7801 ctgcgaaagg tttgccatgc gcacaccggt ggcctcggcc agatcgccta ccgtgcattc
     7861 ttcccgatcg tgcaataact ggagcaactc aaggcgcagc gggtgggcaa acacgccgca
     7921 gagttgagca tggcgtcggt aaaattcttt ctccatttgt aaagcggacc aacctttatg
     7981 taaagcgaac gccggccacc cggcgtagtc attgtaccat atcttctgac gataaactat
     8041 accggaagag acgcctgtaa gcaactgttc agttgcccac gcttctcctt gtagcagcta
     8101 tgcggcacaa aaaacaacag caagtaagag tgctgtgcta caattccgag gtcttgcctg
     8161 gcgcttgctc gcctggctgg ttttgccgca gccgttgcac gatcacggcg cgcatctggc
     8221 ggctgatata gcctagctct tcccagcgat tgagcaattg ggcaatctct tcgcgtgtac
     8281 gctggggatc gctttgtaac atcttgacgc agctatcgat cacgacgtcc agttccatac
     8341 tcatgcctct gctctcctca tctctggctc tgccgaacga tacaacttgc gcaggtacgc
     8401 gtaggcccgg ttcagagcag ccatgcgctc tgtcgatccg gctccctgcc ggtcggggtg
     8461 cacgagcttg gcgtaggctc gatacgcgcg ctgtatctca gcaagcgtcg ctcctggcga
     8521 tagtcccagc aggtgtaggg cttcatccaa ctgcgatagc gcatacccat ttccaggtaa
     8581 gccgtccagg tttgcccacc agtctgcctc cgcctgttta tgccgggcgc gttctcgctg
     8641 acgctgctct tccaggtcaa ggaaagcctg ttcaaatgcg cgcatgttct cctctggatc
     8701 ggccaggaag cgctcgatgc cgctctgcgt ccccaggccg gtaacacggt agtagtcgta
     8761 ccggccgtag cgacgctgcc atttgcgcag gcgctcacgg tagctcgaag gcagcgcttc
     8821 atgctgctgg tcgatccact ggcgataggc gcggtggaaa cggtcaacac agtcgcgctg
     8881 gcgctgcaaa gccgcgttgt aggcgcgcag ttcttcaagc agcgcgccgc gatcatctac
     8941 gtcaagccca tccagcaggc agagatcaag gtcttcaaac ctgccccaat gaagcgtgtg
     9001 agcaaactgc tggaacgctc cgaccgggac ccagagggcc tgctcgttgg tatagtgatc
     9061 aaggatagcg gctgcccgcg ctgagacgcc cctgagggcg ctcagacagg cgcgctcgtt
     9121 cggcatggcg cgacctcctt gttacgagag gactgctgtt gtcgcaggtg gccccgtgtc
     9181 gatcagataa acagggaaac gtcggcatct ttggcaaagc cgaggaagtg cgtcgctccg
     9241 cacacctcct gaatgccgtc gatgaagtcc tcggccttat agccgaacac atccatcgtc
     9301 atgccgcacg gccacagctt gacgcccgac tcgatggcca tctccatcag ttcgtcaata
     9361 gtggagacgt ggtggctctt gaacatgcct ttcatgacaa tcgttcccat ggccgtcatg
     9421 cctgggatcg cgccgacgat gttggggaca ggcatgggca tggcgggatt cgccaggggc
     9481 gcgacccgca ggtgcttact cttctctttg tggatgatgt tcagcccgta gaaggtgaag
     9541 aaaatgccga catcccagcc taacgccgcc gctgttgttg ccagaatgag cgggggatag
     9601 gcccagtcga gcgtcccttt cgtggcgatc atcgccaacc ggggagtgcg cgtctccttc
     9661 tcggcctcct tctcctgcag gtaggtatcg acctgttcgc gtaccaggcg tgcgatgtcg
     9721 ctggaaagac ccggcgcctg gctttctgtc tgcatatcca tagcgtgtca ctccttctgc
     9781 aggcgtccgg cctgcgcgtg agagatgcct tcgcagcacc ccaactcata acgtaccttc
     9841 cattcaatgc ttcaaccgta gagatgcccg ccaattgttg tcagtcggcg cagcggtagc
     9901 attgcacatc ccgttcgaca attttctctt tatagcgttc aaatgcctgg tcatgaggca
     9961 gcaggctcgg ccattcttcc tcggtcttcg ccggcctgag gcgaatcagc cagccggcgc
    10021 catacgggtc tttgttcgcc agcaggatat cccgcttaaa ctccgtttca tttgtagcga
    10081 caatttcgcc gctcaggggt gtggggaatg gtccgaccca cttggcgctc tcgatggttg
    10141 ccgcgctctg accgcgctgt agtgtgcgac caaccgcctt gaagcgtaca tgcacaaatt
    10201 taccgcactg cgcctgcgcc ggatcggtca tgcccatggt gacgcagcca tcctcgtcgc
    10261 gtcgcaccca gatatcgcgc tcaacgtcgt agaaacaatc cgccgggaat tcacagccgc
    10321 gatagatgtg cgcagtcacc cgatgccttc ccttctttgg agtctattga caggcaatgc
    10381 ccttatcgtc caacgcctgc tggtagacag ccacgccctg gtctcctgta accaggtctt
    10441 tgctctcttc atcccagtcc agcggtttca gtttgacgat ccagcccgcc ccataggggt
    10501 cctgattgat cagtgcggga ttgcccttga gcgcatcgtt gacggcgacc agttcgccgc
    10561 ccaccggcgc gggtaccggc ccgacccatt tgccgctctc gaccgtcgcc gcgctctgtc
    10621 cctttgcgag cgccctgccc actttctttg ggctgaccgc gatgactttg ccggccaggt
    10681 gctgcgcgac atccgtaata cctactgtca ccagggcatc aggctcgcgg cgcgcccaga
    10741 cgtgtttgtc caccaggtag tacagatctt ccgggatatt gcaaccgttc gctgttccca
    10801 cgatttcctc caacttacgc ggacgctata cacgacgcgc tacgtgtgca tatcttcgat
    10861 gtgatataac aacagatcaa aagccaccag ccgtatctcg cgttcaaatg cttgcagaaa
    10921 ggtcggatcg cgcccgcgtg gattgacctc tcgctcgcta tattcggcgc attgcgggca
    10981 atccaggcgg tagaagagcc tgcctgtttg ctcatcacga cgcgctatca ctttatcgcc
    11041 gtgttgatcg gtcaggtgcg cgtgaatcac ctggcgcgaa ccttcaacat cgcaatacgg
    11101 gcagcgcatt cctacagtcc ttccgcagtc tgactgcttg ctgctacggc ggactgggtt
    11161 tgcagagctt cgtggacaag ttcgatgata tccttgacga caatctgccc ttcgtacccg
    11221 gatgttttca cggcatccgt gtacatggtg acatctttgg gacaggcgac gacaaaatag
    11281 tccacgttcc ccagcgccac ggcctcgcga atgcggttca cgctcggtcg ctcctggatg
    11341 tttgtctcgg tcatccagat gcgcccgccg ccggctccgc agcagaaact gttctcacgg
    11401 cagcgtccca tttctgtgac ctgcaatcct aacgttttca ggatgtttct cggcgcgtcg
    11461 aacacgcgat tgtaccgccc taaataacag gggtcgtgat aggtcacatg atagctcagc
    11521 gggcgttcga gccgcaattg cccgctctca atcaaccggc atagcagctc ggtatagtga
    11581 attacttcgt agttgcctcc taattgcccg tattcattgc gcagagcgtt cagactatgc
    11641 gggtccgtgg tcacgatggt tttgaacgcg gccttgccca gcgacttcat
//
LOCUS       DASZAK010000254         5142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_361988, whole genome shotgun sequence.
ACCESSION   DASZAK010000254 DASZAK010000000
VERSION     DASZAK010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361988"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1461
                     /locus_tag="VGT44_04375"
     CDS             <1..1461
                     /locus_tag="VGT44_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580069.1"
                     /translation="THVEQFSLHEFDKVSTASLITRTTNDTTQVQQVTIVLLNMIITA
                     PITLVAGVILALNQDIGLSWILVVAIPILAGTIALLMSKAIPIFRAMQVKLDKLNLVL
                     DEGLTGVRVVRAFDRIKHEEQRFDEANLELTNIAIKVNRLIATLMPMMMLVLNISSVA
                     ILWFGAIRINNGDLQLGAMIAFLQYAMQILFALLMVSMMFIMLPRAAASADRINEVLA
                     IEPEIKDADQVRLADRQRGYVEFRDVTFSYPGAEEPALSNISFRARPGQVTAIIGGTG
                     AGKSTLVSLIPRFYDIDSGHILVDGVDVREMSQEHLRSKIGFVPQKAVLFSGTITQNI
                     RYGKEDATDEEIRHAADVAQASEFIVDMKDGFDSVIAQGGTNVSGGQKQRLSIARALV
                     RRPEIYVFDDSFSAVDFKTDAKLRAALKQEIYDATALIVAQRVSTVMDADQIIVLDEG
                     RVAGIGTHRELMKTCEVYREIVSSQLSLDELAEEIA"
     gene            1458..3359
                     /locus_tag="VGT44_04380"
     CDS             1458..3359
                     /locus_tag="VGT44_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580070.1"
                     /translation="MSEERKPQRPAMQQGGPMGRGGWGAMGMPVEKAKDFRGTLFRLF
                     RYFLPQKYRLLIVVIAAIIGTVFNIVGPKILGLATTKLFDGVVAKFVAFQHHLPAPGI
                     DFNYIGTVLVILLGLYLVSSVFMYIQQYVMAGVAQKTMYRLRREVDEKLSRMPIKYFD
                     TRTHGEIMSRAINDMDNLSTTLQQSITQLISSAVTLLGVVVLMLTISPLLSLIVVLTL
                     PLSLVVTMGIAKRSQNYFRRQQRSLGELNGHVEEMYTGHKIVKAFGHEKQSVEEFTVL
                     NEKLYNASWRAQFVSGIIMPLMRFIGNIGYVFVAVVGGVMITQNAIAIGDVQAFIQYA
                     QQFTQPITMLANIANVIQSAMASAERIFELLDDQEEVPEAAGAKVIEHPEGSVQFEHV
                     RFGYAEDNILMEDMNIDVQPGQMIAIVGPTGAGKTTLVNLLMRFYEVNDGQILVDSVK
                     ITDMKRGALRRTFGMVLQDTWLFNGTIRDNIAYGREGATDEEIRNAAKAANADHFIRT
                     IPEGYNTVLNEDATNISQGQKQLLTIARAFLADPEILILDEATSSVDTRTEMQIQKAM
                     AELMKGRTSFVIAHRLSTIRDADLILVMNHGTIIEKGTHEELLAKNGFYADLYNSQFT
                     GRVLEEEAV"
     gene            3579..4283
                     /locus_tag="VGT44_04385"
     CDS             3579..4283
                     /locus_tag="VGT44_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2580071.1"
                     /translation="MRNRPRVLVVDDSPSTCLFIASTLEQAGYDVEVALKGQECLSKV
                     VTFQPYCLILDVMLPDINGYAVCRHVQQSMPERSVYIILISAKNAPLDQSYGLRQGAN
                     RYLPKPFTAEMLVHAVWEGVPARMRPSVHSSPPTTPQPGNLPPLVGLIPRRVDKQGAM
                     RTSNPFDLAPAIEDKQARRLYEAINGQRTLAELSAVMGLDAEAVARPLRVLLKENCIR
                     IYDPAGRLVEDALVRQ"
     gene            4319..4951
                     /locus_tag="VGT44_04390"
     CDS             4319..4951
                     /locus_tag="VGT44_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580072.1"
                     /translation="MNSKEITDLIIGFQAIVTFFISMRAFYLYFKSHRDILFIVGLSM
                     AVIFLGGIAGVIGDYLITSTAFNTFWFRYIGQIVSYTFIFLISLPGAERFMRLLKWLN
                     IIASFVMLSLLAFTPLLPPIPGVELTEILSGARGVACLAVFFNYYAVLYGMKGTRFSR
                     LMCIAFLLITVGIAIYSLKFSVANPLPYDYVGDSIRAVGLVLMLAAFFVG"
     gene            complement(4957..>5142)
                     /locus_tag="VGT44_04395"
     CDS             complement(4957..>5142)
                     /locus_tag="VGT44_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580073.1"
                     /translation="RAQLHLRQILAYLDVPVITKPEVFIINGWEKFDNEGHLTDERGQ
                     REMKLLLERLVRAALTE"
BASE COUNT         1138 a         1401 c         1407 g         1196 t
ORIGIN      
        1 acgcatgtcg aacagttttc gctgcacgaa ttcgacaaag tcagtaccgc ctcgctgatt
       61 actcgcacca ccaacgacac aacacaggta cagcaggtga cgatcgtttt actgaacatg
      121 ataatcaccg ctccgatcac attggttgcc ggggttatcc tggcgctcaa ccaggatatc
      181 gggctgtcct ggatactggt ggtagccatc ccgatactcg ccggcaccat cgcgctgctg
      241 atgagcaaag ccatcccgat attccgcgcc atgcaggtca agctggataa actcaacctt
      301 gtgctggatg aggggctgac cggtgtgcgt gtcgtgcgcg cgtttgaccg catcaagcac
      361 gaagagcagc gttttgacga ggcgaatctt gagttgacca acattgccat caaggtcaac
      421 cggctcatag cgacactaat gccgatgatg atgctggtat tgaacatttc cagcgtagcg
      481 attctctggt tcggcgctat ccgcatcaat aatggggatt tgcagcttgg ggcgatgatc
      541 gccttcctgc aatatgccat gcagattctc ttcgcgctgc tgatggtctc gatgatgttt
      601 atcatgttgc cacgcgctgc ggcttccgcc gacaggatta acgaagtgct ggcgatagag
      661 cccgaaatca aggatgccga ccaggtgagg ctggccgaca ggcagcgagg gtatgtcgag
      721 ttccgggacg tgacattcag ctatcccggc gcggaagagc cagccctctc caacatctcc
      781 ttcagagcgc ggcccggcca ggtgacggcc atcatcggcg gcaccggcgc gggcaaatcg
      841 acgctggtca gcctgatccc gcgcttctac gatatcgata gcggccacat ccttgtggat
      901 ggcgtcgatg tgcgcgagat gtcccaagag cacctgcgct cgaagatcgg cttcgtgcca
      961 caaaaggccg tgctattctc cgggactatc actcagaaca tccgctatgg caaagaagat
     1021 gcgacggatg aagagattcg ccacgcggct gatgtcgcgc aggccagcga gttcatcgtg
     1081 gatatgaaag acggcttcga ctcggtgatt gcccagggcg gcacaaatgt ctccggtggt
     1141 cagaagcagc gtctctccat cgcgcgcgcc ctggtgagga ggccggaaat ctacgtgttc
     1201 gacgacagtt tctcggcggt cgatttcaag accgatgcga agctgcgcgc ggcgctgaaa
     1261 caggaaatct acgatgctac cgcgctgatc gtggcgcagc gcgtcagtac ggttatggat
     1321 gctgaccaga ttatcgtgct tgatgaaggg cgtgtagctg gcattggcac ccaccgggaa
     1381 ttgatgaaga cctgtgaggt ctatcgtgaa atagtgtcct cgcaattgtc gctagatgaa
     1441 ctcgcggagg aaatcgcatg agtgaagaac gcaagccaca gcggcctgct atgcagcagg
     1501 gcggcccgat gggcaggggc ggttggggcg ctatgggcat gcctgtggag aaagccaagg
     1561 acttccgggg aacgctcttt cggctcttcc gctacttcct gccgcaaaaa tatcgcctgt
     1621 tgattgttgt aatcgctgcc atcattggca cagttttcaa catcgtcggc ccgaagatcc
     1681 tgggcctggc gactaccaaa ctgtttgacg gcgtagtcgc gaaattcgtg gcatttcagc
     1741 atcacctgcc cgctccaggt atcgatttca actatattgg aacggtgctg gtgatcttgc
     1801 tgggcctgta cctggttagc tctgtcttca tgtacatcca gcagtacgtc atggcgggcg
     1861 tagcgcaaaa gacaatgtac cggctgcgca gagaggtcga tgagaagctg tcgcgtatgc
     1921 caatcaagta ctttgacacg cgcacacacg gcgaaatcat gagccgcgcc atcaacgaca
     1981 tggataacct cagcacaacg ctgcagcaga gcatcacgca gttgatctcc tcggcggtca
     2041 cactgctggg cgtggttgta ctgatgttga ccatcagccc gctgctgagc ctgatcgttg
     2101 tcctgacgct gccgctcagc ctggttgtca caatggggat cgcgaaacgc tcgcaaaact
     2161 acttcaggcg gcagcagcgc tcgctcggcg agctgaacgg ccacgtggag gagatgtata
     2221 ctggccataa gatcgtcaag gcttttggac acgaaaagca atccgtcgag gaatttaccg
     2281 tactcaacga gaaactctat aacgcaagct ggcgcgcgca gttcgtctcc ggtatcatca
     2341 tgccgctgat gcgcttcatc ggcaacatcg gctacgtctt tgtggcagtc gtcggcggcg
     2401 tcatgatcac gcaaaacgcg attgccattg gcgacgtaca ggcgtttatt caatacgccc
     2461 agcagttcac acagccgatc accatgctgg ccaatatcgc taacgtgatc cagtccgcta
     2521 tggcatcggc ggagcgcatt tttgaactgc tcgacgatca ggaagaggtc ccggaggctg
     2581 ccggcgcgaa ggttatcgag catcctgagg gaagtgtcca gttcgagcat gttcgctttg
     2641 gctatgccga agacaacatc ttgatggagg acatgaatat cgatgtccag cccggccaga
     2701 tgatcgccat tgtcggcccg accggcgcgg gcaagacgac gctggtcaat ctgctgatgc
     2761 gcttctacga ggtcaacgat ggccagattc tggtggatag cgtgaagatc acggatatga
     2821 agcgcggcgc gctccgccgc accttcggca tggtactcca ggatacctgg ctcttcaatg
     2881 gcacgatccg cgacaacatc gcttatggcc gtgagggtgc cacggatgag gagatcagga
     2941 acgcggctaa ggcagctaac gccgatcact tcatcaggac gataccggaa ggctacaaca
     3001 ccgtcctgaa cgaagatgcg accaacatct cccagggtca aaaacagttg ctgacgattg
     3061 cccgcgcatt cctggccgat ccagagatcc tgatcctgga cgaagccacc agcagcgtcg
     3121 atacccgcac cgagatgcag attcagaagg ccatggccga actgatgaag ggccgcacca
     3181 gcttcgtcat cgctcaccgg ctctccacca tccgtgacgc cgacttgatc ctggtcatga
     3241 atcacggcac aatcatcgaa aaaggcaccc acgaggaact gctcgcgaag aacggcttct
     3301 acgccgacct ctacaacagc cagttcaccg gtcgcgtgct tgaagaggaa gccgtgtaaa
     3361 actgcccgta ggggcgttgc acagaaaagc acatcaacta cacagttgat gtgcttttct
     3421 gttaagattt cttaatgccg gactggtact tttctgttac ttctcaatat gccgatgctt
     3481 aaatctgctt agcggtagat tatgatttca tcgtaataga tgtgcaatgt gcatcattgt
     3541 gtttcaacat gctgtgacag ggaagaaagg gagaaacagt gagaaatcgc ccgcgagtcc
     3601 tggtcgtcga tgatagtccc tcgacctgtc tttttattgc ctccacactt gagcaggctg
     3661 gctatgatgt ggaagttgca cttaaggggc aggagtgtct ttctaaagtg gtgacgttcc
     3721 aaccttattg ccttatcctg gatgtgatgc tacccgatat caatggctac gccgtctgcc
     3781 ggcatgtgca acagagtatg ccggagagat cggtttacat catcctcatc agcgccaaaa
     3841 atgctcctct cgatcagagt tatgggctgc gtcaaggagc taatcgctac ttgcctaaac
     3901 cttttaccgc ggagatgctt gtccatgcgg tctgggaggg cgtcccagca cgtatgcgtc
     3961 cttccgtcca cagctcgcct cctaccacac ctcaacccgg caacctgccg ccattagtcg
     4021 gactcatacc gcgccgtgtt gacaaacagg gcgcgatgcg aacgagcaat ccttttgatc
     4081 ttgcccctgc cattgaagat aaacaggccc gtcgtttata tgaagccatc aatggacaga
     4141 gaacgctggc tgagctttcc gcggttatgg gacttgatgc cgaagcagtt gccaggccac
     4201 tgcgcgtcct gctcaaagag aactgcattc gcatctatga tccggcaggg cgacttgtgg
     4261 aggatgcgct cgtaaggcaa taaagcgcct cccacaatca catataagga gagactagat
     4321 gaactcgaag gagatcaccg acctcattat tgggtttcag gctatcgtca ccttcttcat
     4381 ttcgatgcgt gccttctatc tctactttaa atcgcatcga gacatcctct tcatcgtcgg
     4441 attatcaatg gcggtgattt ttttaggcgg gattgccggg gtgattggag attacctgat
     4501 taccagcacc gctttcaata ccttctggtt tcgctatatt gggcaaatcg tcagctatac
     4561 gttcatattc cttatctcgc tgcccggcgc agaacgcttt atgcgcctcc tgaaatggtt
     4621 gaacatcatt gcctcgtttg tgatgttatc actgctcgct tttactcctc ttcttccgcc
     4681 tatccccggc gtggaattaa cagaaattct cagtggcgct cgtggcgttg catgcctggc
     4741 cgtgttcttc aattattacg ccgtcttgta tggcatgaaa ggcacgcgct tcagccgttt
     4801 aatgtgcatc gccttcctgt taataacggt cggcatcgcg atctattctc tcaagttttc
     4861 tgtggcgaat ccattgcctt acgactatgt aggggatagt atacgcgccg ttggcctcgt
     4921 gctgatgctt gccgcctttt tcgtcggatg atttcgttac tctgtaagag ccgcgcgtac
     4981 cagtcgttcc agcagtagtt tcatttcgcg ttggccgcgt tcgtcggtca ggtggccctc
     5041 gttgtcgaat ttctcccagc cgttgatgat gaacacctcc ggtttggtga tgacgggaac
     5101 atccaggtag gcgagaatct ggcgcaggtg tagttgcgcg cg
//
LOCUS       DASZAK010000255         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_6575, whole genome shotgun sequence.
ACCESSION   DASZAK010000255 DASZAK010000000
VERSION     DASZAK010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6575"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VGT44_04400"
     CDS             complement(<1..367)
                     /locus_tag="VGT44_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV2580074.1"
                     /translation="MQVGFIGIGAMGRPMTLNLLKAEYQVTIFARHPENAEVQEVINA
                     GAKLAPTPRMVAMASDIVITMLPNSPQVEEVVSGPHGILEGARKGLIIVDMSTIAPAT
                     SRKLAQVASTRGTHFLDAPV"
     gene            complement(394..2412)
                     /locus_tag="VGT44_04405"
     CDS             complement(394..2412)
                     /locus_tag="VGT44_04405"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV2580075.1"
                     /translation="MFNKILIANRGEIAIRIMATCREMGVGTVAVYSEADRHARHVQA
                     ADEAYLIGPAPAAQSYLRIEAIIEAARKSGAEAIHPGYGFLSENAAFVEACDAAGIVF
                     IGPPAAAMRLMGSKIAAKQIAQSVGAPTVPGYNGAQQDDETLFAEARRTGFPLLIKAS
                     AGGGGKGMREVHDVRDFLEQLAGARREALNAFGDGTIFLERLIQQPRHVEFQVLGDTY
                     GNLVYLGERECSIQRRHQKIVEESPSVALSPTLRAAMGEAAVRIAKAAGYVNAGTLEF
                     MLDVDQRFYFLEMNTRLQVEHPVTELVTGLDLVRHQIRIAAGEALEMAQEQIQPRGHA
                     IEARLYAEDPEQNFLPSTGAIAAFLPPAGPGVRVDSGIASGDEITQFYDPMVAKLIVY
                     GEDRPAAIARLQAALEQTVTLGVKTNAALLLAIARHSAFQDGLTHTHFLDEYGLIEAE
                     QQRTSQAPDLYAFYAAAFYDILHVSEAARNGAQTGQAVNPWQALGPWRMVGEGRSITY
                     HYGGQAYRGTLSSSPGAAGTWTIQAGNHAAAHIGCIFGSDGLVLMREGSQQRRAYVAR
                     VEGQTQVAMHGQTYRFDRRQPPDIDATAHGGNVAHSQKTLTAPMAGTIVKVQARDGQF
                     VQQRQVLVILSAMKMEHTIAAPYEGTVRRVYYGEGDVVKGGAVVVEME"
     gene            complement(2413..>3161)
                     /locus_tag="VGT44_04410"
     CDS             complement(2413..>3161)
                     /locus_tag="VGT44_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2580076.1"
                     /translation="KVATVTMRRAEARNSINTQLVQDLLAAFAELRSETRLHAVVLTG
                     DGPAFSAGADLHMMQAAAAFTEEQNLRDALTMAGLFSAINNFPCPVIARVNGAAMGGG
                     LGMVAVCDIVVAVETAQLSFSEVRLGIAPAVISPYVLRKIGESQARMLFVTGEVFSAA
                     YAREIGLVHIVTTADELDAAVEKKVNELLRAAPLAMRACKALALNVGRMDAADASRYT
                     AETIARLRVSKEGQEGLRAFLEKRKPGWIE"
BASE COUNT          534 a         1035 c          977 g          615 t
ORIGIN      
        1 tgaccggggc atcgaggaag tgcgtaccgc gcgtcgaggc tacctgcgcc agcttgcggc
       61 tcgtggcggg cgcgatggta ctcatatcca cgataatcaa ccccttgcgc gcgccttcca
      121 ggatgccgtg cgggccgctc acgacctctt ccacctgcgg cgaattgggc agcatcgtaa
      181 tcacgatatc agaggccatc gctaccatac gcggcgtggg cgcgagcttt gcgcctgcgt
      241 tgatgacctc ctgcacctcc gcgttttcgg gatggcgcgc aaagatcgtg acctggtatt
      301 ccgctttaag caagttgagc gtcatgggcc gtcccatcgc gccgatgcca ataaatccta
      361 cttgcatgat ttcacttttc tttctttgtt gtattattcc atttcgacga cgaccgcgcc
      421 gcccttgaca acgtcgcctt cgccgtagta gacgcgccgc accgtaccct cgtaaggcgc
      481 ggcaatcgtg tgttccatct tcatcgcgct cagaatcacc agcacctggc gctgctgtac
      541 gaattgtcca tcgcgcgcct gcactttgac gatagttccg gccattggcg cggtcagcgt
      601 tttctgcgag tgcgccacgt tgcccccgtg cgccgtcgcg tcgatatcgg gtggctgccg
      661 tcgatcaaaa cggtaggtct gcccgtgcat cgccacctgc gtctgcccct caacgcgcgc
      721 aacataggcg cgtctctgct ggctgccctc gcgcatcaga accaggccat cgctgccgaa
      781 gatgcagccg atatgcgctg cggcatggtt gcccgcctgg atcgtccatg tccccgccgc
      841 gcctggcgac gaactcagcg taccgcggta cgcctgtccg ccatagtggt aggtgatgct
      901 gcgcccttca ccgaccatgc gccaggggcc aagcgcctgc cagggattga ccgcctggcc
      961 ggtctgcgcg ccatttcgcg cagcttcgga aacgtgcagg atatcgtaga atgccgccgc
     1021 gtagaaggcg taaaggtcgg gcgcttgcga cgtgcgctgc tgctcggcct cgatcaaacc
     1081 atattcgtcg agaaaatgag tgtgggtcag tccatcctgg aaagcggaat ggcgcgcaat
     1141 cgccagcagc agcgcagcgt tggtcttgac gccaagtgtg accgtctgtt ccaacgctgc
     1201 ttgcaggcgc gcgatggctg ccgggcggtc ttcaccgtag acgatcagtt tggcgaccat
     1261 cgggtcataa aattgcgtga tctcatctcc gctagcgatg ccgctgtcaa cacgcacgcc
     1321 tggccccgct ggcggcagga aggcagcgat tgcgccggtg gagggcagga aattctgctc
     1381 agggtcttcg gcatacaggc gcgcctcgat ggcgtggccg cgtggctgga tctgctcctg
     1441 cgccatctca agtgcttcac cggcggcaat gcgtatctgg tggcgcacca ggtcaagtcc
     1501 cgtcaccagt tccgtgacag gatgctcgac ctgcaagcgc gtattcatct caagaaaata
     1561 gaagcgctgg tccacatcaa gcatgaattc gagtgtcccg gcgttgacgt acccggctgc
     1621 ctttgcgatg cgcacggcag cctcgcccat cgcggcgcgc agcgtgggcg acagcgcgac
     1681 ggacggactc tcctccacga tcttctggtg gcggcgctgg atcgagcatt cgcgctcgcc
     1741 caggtagacc aggttgccgt aggtatcgcc cagcacctgg aattcgacat ggcgcggctg
     1801 ctggatgagc cgttcgagga agatggtgcc atccccgaag gcgttcaacg cctcgcgcct
     1861 ggccccggcc agttgttcca ggaaatcgcg gacatcatgc acttcgcgca tccctttgcc
     1921 gccgccgcca gccgaggctt taatcagcag gggaaagccg gtgcggcgcg cctcggcaaa
     1981 gagcgtctcg tcgtcctgct gcgcgccgtt gtagccgggc acggtgggcg cgccgaccga
     2041 ttgcgcgatc tgcttggcgg cgatcttcga ccccatcagg cgcatcgcgg ctgccggcgg
     2101 cccgatgaag acgatgcccg ccgcgtcaca cgcctcgacg aaggccgcat tctccgagag
     2161 gaagccgtag ccggggtgga tggcctccgc gccgctcttg cgcgccgcct cgatgatggc
     2221 ctcgatgcgc aggtagctct gcgcagcagg cgcggggcca atgaggtatg cctcgtcggc
     2281 agcctggaca tgccgggcat ggcgatccgc ctccgagtag acggcaacag tgcctacgcc
     2341 catctcgcgg caggtcgcca tgatacgaat ggcgatttca ccccggtttg caatcaagat
     2401 tttattgaac atttactcaa tccagcctgg tttgcgcttc tccaggaagg cacgcagacc
     2461 ctcctggcct tccttgctga cgcgcaggcg ggcgatggtc tccgccgtgt agcggctggc
     2521 gtcggcggcg tccatgcgcc cgacgttgag cgccagggct ttgcaggcgc gcatagccag
     2581 cggcgcggcg cgcagcagct cgttgacctt cttctcgact gccgcgtcga gttcatccgc
     2641 tgtcgtgacg atatgtacca gcccgatctc tctggcgtag gccgcgctga agacttcgcc
     2701 cgtcacgaag agcatgcgcg cctgcgattc gccgattttg cgcagcacgt agggcgagat
     2761 cacggcgggc gcgatgccga gccgcacctc gctgaacgag agctgcgccg tctcgacggc
     2821 aactacaata tcgcagacgg ccaccatccc cagcccgccg cccatggccg cgccgttgac
     2881 acgagcgatc actgggcagg gaaagttatt gatcgcgctg aagagtcccg ccattgtaag
     2941 cgcatcgcgc agattctgct cttccgtaaa tgccgccgcc gcctgcatca tatgcaggtc
     3001 ggctccagcg ctgaaagccg ggccgtcgcc ggtcagcacc acagcgtgga ggcgtgtttc
     3061 agagcgcagt tccgcgaagg ccgccaggag gtcctgcacc agttgcgtat tgatcgagtt
     3121 gcgagcctcc gcccgccgca tggtgacggt ggctaccttg c
//
LOCUS       DASZAK010000256         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_397267, whole genome shotgun sequence.
ACCESSION   DASZAK010000256 DASZAK010000000
VERSION     DASZAK010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397267"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..358
                     /locus_tag="VGT44_04415"
     CDS             119..358
                     /locus_tag="VGT44_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580077.1"
                     /translation="MENQTNTAGFFPLVTRLRNDSALHGRTMSAVGVKFDTGITIPGS
                     IATCGIGLRNAGPRSAFAQGRSMPISRGSPPIYDE"
     gene            572..1528
                     /locus_tag="VGT44_04420"
     CDS             572..1528
                     /locus_tag="VGT44_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580078.1"
                     /translation="MAIQPLEESRLLEGIAIDPNKDAVEFLGVTKVFKKSKPLFRWPG
                     SPRKEGKKREVRAVDDVTLIVKRREIVGILGANGSGKSTLVRILSTLLIPDTGVVRIF
                     GYDVTRDERMVQKLINRVSVEASFFKKLSPMENLIYAARLYNMPGREARVKIRSILER
                     LGIPKDRIGQPLENMSRGMQQKVAIARAFLTAPIVLLLDEPTTGLDPRSKRDVQVFVR
                     QLREDHDATILITTHDMDEAEALCDRVAIIDQGRIVAMGTVEELKQAVSERLERTTPV
                     TMNEVFMHYTAAHLITEADIERYGSWEKAFEAFEAQKEEEDD"
     gene            1586..2416
                     /locus_tag="VGT44_04425"
     CDS             1586..2416
                     /locus_tag="VGT44_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2580079.1"
                     /translation="MSSMLHRGRASYAFFERNWNLTKRYWGWEVVWLIYNVVNAMSVT
                     YIGKAAQVLTNTKVDTNFLVLYLMIGTAVWSYLSVTFEGVTDLITIERWEGTIEHTFM
                     APISRFTHLVGSCWYAVAHGMLFTFLQLIVVAAFFQLDLSHANYLTAVFMLLMGSVSF
                     IGFGIGASVLPLLFTERGSQMSYIVRSVLLLVSGVYYPISVLPGWMQPLARISPATYV
                     LEGLRAALLNNQVIWSGEIWSYTWPLILTGIVSVPLGVYLFRLAERYAKRTGKLKRNG
                     "
     gene            2491..>2865
                     /locus_tag="VGT44_04430"
     CDS             2491..>2865
                     /locus_tag="VGT44_04430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2580080.1"
                     /translation="MNHTLPQGFQLRRPGMDDLKPVHHLIELYDVAEFGGQEITLEDM
                     QTFWTTPTFHLDTGAWVVEASDGLVVAYADVEQNEHARLHTFVRVHPEYQDIGIGEYL
                     YELAGEWAAQQIPLATPEVRITL"
BASE COUNT          634 a          804 c          832 g          595 t
ORIGIN      
        1 gcgggattcc gtatactcgc aacagtagga aaagagactg actacttctg ggctgcgaca
       61 ggctgaatgc ctgtgatcgg cagcattcac ccaatcttat gaaaggagac ggctgggcat
      121 ggaaaaccag accaacacgg cgggcttctt cccgctcgta acccgcttgc gcaacgattc
      181 tgctctgcat ggccggacta tgtccgccgt cggggtgaag ttcgataccg gcatcactat
      241 tcctggctct atcgccacgt gcggtatcgg cctgcgcaat gcagggcctc gttctgcctt
      301 cgcccagggc cgcagtatgc ccatttcaag ggggtcgcca ccgatttacg acgagtgagt
      361 gaccggtgag catcaccctt gaccgtgggc gactgaggat aacgaaaatt cagtcccacg
      421 gttttttatt gctctttttc attccgtagc gttaccacat aaagtcacga aaggattaga
      481 acgggtgtat gaaaatcggg gaatagctga cagttgctgt caagaatagt cacgtaatat
      541 tgaaatagca agaaagggag caaaactacc gatggcaata caaccactcg aagaatcgag
      601 actgctggaa ggtatcgcga tagatccaaa taaagatgcc gtggaatttc tcggagtcac
      661 aaaggtattc aagaagtcga agcccctgtt tcgctggcct ggatcgccac gtaaagaggg
      721 caagaagcgc gaggtgcgag cggtcgatga tgtcacactg atcgtcaagc ggcgtgagat
      781 tgtcggcatc ctgggcgcga atggttccgg gaaatctacg ctcgtgcgca tactctcgac
      841 cctgctcatc ccggataccg gggtggtacg tatctttggc tacgacgtga ccagggacga
      901 gcgcatggtt cagaagctca tcaatcgcgt cagcgttgaa gcctcgttct tcaagaagct
      961 ttcgccgatg gagaacctga tctacgcggc tcgtctctac aacatgcccg gcagggaggc
     1021 gcgcgtgaag attcgcagca tcctggaacg cctgggcatc ccgaaggacc gcatagggca
     1081 gccgctggag aacatgtcgc gcgggatgca gcagaaggtc gccatcgcgc gcgccttcct
     1141 gaccgcgccc attgtcctgc tgctggacga gccgacgacc ggccttgacc cgcgctcgaa
     1201 gcgcgatgtg caggtctttg tgcggcaact gcgcgaggat cacgatgcga ccatcctgat
     1261 tacaacgcac gacatggacg aggccgaggc attgtgcgac cgtgtcgcca tcatcgacca
     1321 gggccgtatc gtggcgatgg gtaccgtcga ggaactcaag caggccgtct cggagcgtct
     1381 cgaacgcacc acgccggtta ctatgaacga agtctttatg cactacaccg ccgctcacct
     1441 catcaccgag gccgacatcg aacgctatgg aagctgggaa aaagcttttg aagcattcga
     1501 ggcccaaaaa gaggaagagg acgactaaca acccgcatag ggtaggggcg accctcgcgg
     1561 tcgccctgga accgggagat acatcatgtc atccatgctt catagaggac gcgcatccta
     1621 cgccttcttt gagcgaaact ggaacctgac caagcgctac tggggctggg aggtcgtctg
     1681 gctgatatac aacgtcgtca acgccatgag cgtgacctat atcggcaagg cagcccaggt
     1741 ccttactaat acaaaggttg ataccaactt cctcgtgctg tatctgatga tcggcacagc
     1801 cgtctggtcg tacctgagcg tcaccttcga gggcgtgacc gacctgatta ccattgagcg
     1861 ctgggaaggc actatcgagc ataccttcat ggcgccgatc tcgcgtttca cgcacctggt
     1921 cggcagctgc tggtacgcgg ttgcgcacgg gatgctcttc acgttcctgc aactgatcgt
     1981 ggtcgcggcg ttcttccaac tcgacttgag ccacgccaac tacctgaccg ccgtcttcat
     2041 gttgctgatg ggcagtgtct cgttcatcgg cttcggcatt ggcgcgtcgg tgctgccgtt
     2101 gctcttcacc gagcgcggct cgcagatgtc gtacatcgtg cgctctgtcc tgctgttggt
     2161 gtcgggcgtc tactacccga ttagcgtact gccgggttgg atgcagccgc tggcgcgcat
     2221 ctcacccgcg acctatgttc tggaagggct gcgcgcggcg ctgctgaaca accaggtcat
     2281 ctggtcgggg gagatctgga gctacacctg gccgctgatc ctgaccggta tcgtcagcgt
     2341 accactgggc gtctacctct tccggctcgc cgagcgatac gccaagcgca ccgggaagct
     2401 taaacgcaat gggtaatctg cgtcctgacg gtgaaattca ccgtcaggac aaggcagaaa
     2461 aagaatagta atactgagga gaacaatctc atgaaccata cactaccgca gggctttcaa
     2521 cttcggcgtc caggcatgga cgacttgaag cctgtgcatc acctgataga actgtacgat
     2581 gtcgccgagt ttggcgggca ggaaatcaca ctggaggata tgcagacatt ttggaccacg
     2641 ccgacctttc acctggacac aggtgcctgg gtggtcgaag ctagcgatgg gctggtcgtc
     2701 gcttatgccg acgtggagca gaatgaacac gcgcgcctgc acacatttgt gcgcgtgcat
     2761 cctgaatatc aggatattgg catcggcgaa tatctgtacg agcttgccgg agagtgggcc
     2821 gctcagcaga ttccgctggc gaccccagag gtgcgcatca ccctg
//
LOCUS       DASZAK010000257         7459 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_326311, whole genome shotgun sequence.
ACCESSION   DASZAK010000257 DASZAK010000000
VERSION     DASZAK010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326311"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /locus_tag="VGT44_04435"
     CDS             <1..1616
                     /locus_tag="VGT44_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV2580081.1"
                     /translation="ALSDYQDLQYILTLQESVAVAIADGYARATRRPTVVQLHSGVGL
                     GNGIGMMYQAKRGHAPLVVINGEAGLRYDAMDAQMAADVVAMAEPVTKWATRVVDSSS
                     LLRVLRRAIKVAATPPMGPVFVCLPMDVLDAPNEEVVVPTSFPVTRVAPEPEQIAQVA
                     AMLAAARKPMIIMGDGVAASGAQAELTRVAELLGAEVWGADSSEVNISTTHPLFKDLL
                     GHMFGEYSRAITSRADVVLICGTYVLPEVFPALAGVFAPGAKVIHIDLDAHEVAKNFP
                     VDLGLISDPKTTLARLAAALETTMTPEQKAAASTRTGQCVAAREQEVTAQREADRGRR
                     DAVPLHPSRFLEELAAQLPKDAVIFDEALTTSPDLVHYLPPTIPGHYFQTRGGSLGVG
                     IPGAIGLKLAYPDKTVIGFSGDGGSMYTIQALWTAAHHKIGAKFVVCNNHSYELLKLN
                     IQQYWRERHLPEREFPASFDLHDPDIRFDELARAMGVQAVRVETPEQIGPAIRQALAD
                     DDPFLIDLVIANEVPHHVVQKVVSRYGHA"
     gene            1713..2282
                     /locus_tag="VGT44_04440"
     CDS             1713..2282
                     /locus_tag="VGT44_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV2580082.1"
                     /translation="MKLQGKKIGILIESDFYEREIFYYELRFPEEGAELHFLTRLWGQ
                     PSLTFTGHEYKVPFECHESFEQMDDATLRSFSAIIVPSGMVADRLRYTEDINKLPPAT
                     EFLRRAFEEKNILKGFICHGLWLVAPMPELVRGRKVVVHNNLLGDIRNMGAIYTDQDI
                     VVDGDLVTGRTGGHCHLFARTILDMLAER"
     gene            2319..2744
                     /locus_tag="VGT44_04445"
     CDS             2319..2744
                     /locus_tag="VGT44_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2580083.1"
                     /translation="MPALTFSHVAIACKDPLATERFYSKYFGFKRARVIPLGTDQIVF
                     IKSGDTYLELFRAKEQASIPPASQDGPWYPGWRHIAFQVDNVDARLAEMGNDVKITLG
                     PLDFDAFIPGWRTVWIADPEGNIVEISQGYVDQENPTQL"
     gene            2828..4939
                     /gene="glgX"
                     /locus_tag="VGT44_04450"
     CDS             2828..4939
                     /gene="glgX"
                     /locus_tag="VGT44_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEV2580084.1"
                     /translation="METAPLEYQIERGHPHPLGAIPDNNGVNFAVFSERATSVELLLF
                     DKHDDPKPIHTIQLDPDKHKTFYFWHVYVRGLKPGASYAYRVDGPQDLHGRGDRFNRN
                     KVLLDPFASGNTETLWNRANAFGPEDNLATSMRSVVIDLAGYDWEGDRPLDRPLQETI
                     IYEMHVRGITRSPSSGCQNPGLFAGIIEKIPYLKELGITAIELLPVFDFDENEVKQLN
                     PVDGTPLPNYWGYDPFSFFALQSWYCKSPEAGNHYTEFRDMVKALHKAGIEVILDVVF
                     NHTGEGDHRGPTISFRGFANSTYYLLSQQDRQYYMNYSGCGNTINGNHPITEKLIVDT
                     LNFWVKEMHVDGFRFDEAVILCRDENGLPMLYPPVIWHIELEDSLADTKVIAEAWDAA
                     GLYQVGYFPGYRWAEWNGRYRDTIRRFVRGDRGYFDGKTMVGRVADIIAGSASIFESS
                     GELPINSVNFITAHDGFTLNDLVSYNNKHNEANGEGNRDGTDNNLSWNCGVEGETDDP
                     DIEALRKRQIKNFAAILLLSQGIPMFVAGDEIRRTQKGNNNAYCQDNEISWFDWNLAK
                     KNQEMLRFFKQMIALRKSHPLLQRSRFFMGQLNQRGLADISWHGCRLNSPGWYDPNSS
                     VLGFTMGGFDGDVDLHVMLNMDWQNLDFDILSIEGRKWYRAVDTALPSPEDIAEAGQE
                     VEIFGNTYHVNGRSVVVLLSR"
     gene            5025..7241
                     /locus_tag="VGT44_04455"
     CDS             5025..7241
                     /locus_tag="VGT44_04455"
                     /inference="COORDINATES: protein motif:HMM:NF018877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HEV2580085.1"
                     /translation="MDKLSWTSSDTIAGYVTSFERDRKTFGLRTNNDREFRVKITDAT
                     YAELVQNLDEPFQDPRTPLESIITPGRYLFACGIFYPEAGEVKFEAKRIILVGRNADE
                     YRFEVPDWWIRQIRSLAEFYFNAQFPDGTIDYHNYRTHLTLEGQKLPGTRQETDTISR
                     MIYGFATAYMLTGVDRYLEAAERGTEYLREHMRAFDEDRDCVYWYHGMDVIGNRERKI
                     FASQFGDDFDAIPAYEQIYALVGPIQTYRVTGDARILQDAEKTINLFNQFFLDREKGG
                     YFTHIDPVNFDPRAESLAANRARKCWNSVGDHAPAYLINLWLATGDKRFEDFLVNIAD
                     TIVEHFPDYEHSPFVQERFHEDWSHDTTWGWQQNRAVVGHNLKIAWNLMRIHHLRPNE
                     RYTELARKIAAIMPTVGMDRQRGGWYDTMERVRSAGEEWHRFVWHDRKAWWQQEQAIL
                     AYLILYGSLRQPEYRQLARESMAFYNTFFPDHDNGGVYFNVQATGIPFLLGTERFKGS
                     HSMSGYHSFELCYLAAVYSNLLINKQPMNLYFKPKAGAFKDNLLRVMPDILPPGSVRI
                     QQVWLNGDRYQDFDPVAGTVTLPSMQPQQSLAQRPSWAGSPYPMLTTMRELKVRVRLA
                     PTGTLFDALLDLSDEVGQLVLYGDLSEVAAPAFKAHMEKIVAAQPRRVVLRLEDLQTI
                     AEVPARMLCFFSDKFTTNEAIYVVGTNEKVKETLQRVGVWEELNAMETYDELWLRS"
BASE COUNT         1700 a         2046 c         2121 g         1592 t
ORIGIN      
        1 atgccttgag cgactatcag gaccttcagt acatcctcac actccaggag tcggtggcgg
       61 tagccatcgc cgacggttac gcccgtgcaa ccaggaggcc aacagtcgtg caactgcaca
      121 gtggcgttgg cctgggcaac gggattggca tgatgtatca ggccaagcgt ggccatgctc
      181 ccctggtcgt catcaacgga gaggccggcc ttcgttacga tgccatggac gcgcagatgg
      241 cagctgacgt ggtggcgatg gcagagcccg tgacaaaatg ggcgacccgg gtggtggact
      301 cgtcctcgct cttgcgagtg ctgcgtcgtg ccatcaaggt ggcggccaca ccgccgatgg
      361 gacctgtgtt tgtctgcctg cccatggatg tcctcgacgc tccaaatgag gaagtcgtgg
      421 ttcccacctc gttcccggtg acacgggttg ccccagaacc agagcagatt gcgcaggtgg
      481 cggccatgct ggctgctgcc cggaagccca tgataatcat gggcgacggt gtggcggctt
      541 ccggcgcgca ggctgaattg accagggtag ccgaattact tggtgccgag gtctgggggg
      601 cagactcatc tgaagtcaac ataagcacca cgcatccgct cttcaaggat ctgctgggac
      661 atatgtttgg ggagtatagc cgagcaatca catcgcgggc tgatgtggtg ctcatttgcg
      721 gaacctacgt gttgcccgag gtcttccccg cgctcgctgg cgtctttgcc cccggagcga
      781 aggtgatcca cattgatctg gatgcccatg aagtggccaa gaacttcccg gtagatcttg
      841 ggctgatcag cgatccgaaa accacacttg ccagactggc ggccgcacta gagactacca
      901 tgacccctga gcagaaagcg gcggcaagca cccgtaccgg ccagtgtgta gcggccaggg
      961 aacaggaagt gaccgcccaa cgcgaggcgg ataggggcag acgcgatgcc gtaccactcc
     1021 atccctcacg gttcctggag gaattggctg ctcagcttcc gaaagatgcg gtgatcttcg
     1081 acgaggcatt gacaacctct cctgacctgg ttcattatct cccaccaacg ataccgggac
     1141 actatttcca gacacgcggt gggtcgttgg gagtcggcat cccgggagcg attggcctca
     1201 agctcgccta tccagacaaa accgtgatcg ggttcagcgg ggatggcggg agtatgtaca
     1261 cgatccaggc actctggacc gcagctcacc acaagattgg cgccaagttc gtcgtctgca
     1321 acaatcacag ctatgaactg ctcaaactca atatccagca gtactggcgt gagcgccact
     1381 tgccggagcg tgaatttccc gcttccttcg atctccacga cccggacatc cgcttcgatg
     1441 aactggcacg agcgatggga gttcaggccg tccgggtcga gacgccagag cagatcggcc
     1501 cggcgatccg ccaggcgctg gcagacgacg accccttcct gattgacctg gtcatcgcga
     1561 acgaagtgcc gcatcacgtc gtgcaaaagg tcgtgagcag gtatggccat gcgtgaagct
     1621 tgctggggag gaacgctgcg gcgtggtctc ttgttccgac tggcaacgaa ctgcgttgta
     1681 gcttcgctca gccagtcgga aaggaggtaa ccatgaaact gcaagggaaa aagatcggca
     1741 ttctgatcga gagcgatttc tacgaaaggg aaatctttta ctatgagctt cgcttcccag
     1801 aagaaggggc agagctgcac ttcctgaccc gcttgtgggg tcagccctct ttgaccttta
     1861 cagggcatga atacaaggtc ccctttgagt gtcacgaaag ctttgagcag atggatgatg
     1921 ccacgctcag aagcttttca gcgatcatcg tcccgtccgg aatggtggct gaccgtctca
     1981 gatataccga ggatatcaac aaactgccgc cggccactga gtttctcagg cgagcgttcg
     2041 aggagaagaa catcctcaaa gggttcatct gccacggcct gtggttggtg gcgccgatgc
     2101 cggagttggt gcggggtaga aaggtggtcg ttcacaacaa cctgctcggc gatatacgca
     2161 acatgggggc catctatact gatcaggaca tcgtggttga cggcgacctg gtgacagggc
     2221 gcactggggg ccattgccac ctctttgcgc gcacgatcct tgacatgctg gccgaacgct
     2281 aacggtatgc tgatcagtag tttctaagga gagaaacaat gcctgctttg accttttcac
     2341 acgtcgcgat agcctgcaaa gacccgctcg ctaccgagcg attctatagc aaatacttcg
     2401 gcttcaagcg agcgcgagtc ataccgcttg gtacagacca gatcgtcttc ataaaatcgg
     2461 gggataccta cctcgagctt ttccgagcga aggaacaggc ctccatccca ccagccagcc
     2521 aggatgggcc ttggtatcct ggctggcgtc acatcgcttt tcaggtggac aacgtcgacg
     2581 ccaggctggc cgagatgggc aacgatgtga agatcaccct gggtccgctg gactttgatg
     2641 cctttattcc gggctggcgc acggtctgga tagcggatcc ggaaggcaat attgtcgaga
     2701 taagccaggg ctatgtcgac caggagaatc caacacagct atagtctgga ggggcaaaat
     2761 ggctcacaca aaggagagcg agaaccaatc tgccaggcca gcgatggcta tggtaaagga
     2821 gacgatcatg gaaacggctc ctctcgagta tcaaatagaa cggggacacc cccatccctt
     2881 gggtgcgata cctgataata acggtgtcaa cttcgctgtt ttttcggagc gtgccacatc
     2941 tgtcgaactc ttgttatttg acaagcacga cgacccaaag ccgatccata cgattcaact
     3001 ggaccctgac aagcacaaaa ccttctattt ctggcacgtc tacgtgagag gactcaaacc
     3061 aggcgccagc tatgcctatc gcgttgacgg tccacaggat ctacacggga gaggagaccg
     3121 ctttaaccgc aacaaggtgc tgctcgatcc attcgccagc ggcaatacag aaacgctctg
     3181 gaaccgcgcc aatgcttttg ggccagagga caatctagcc acttccatgc ggagcgtggt
     3241 catcgacttg gcggggtatg attgggaagg cgaccgcccc ctggaccgac ctctgcaaga
     3301 gactatcatt tacgagatgc acgtgcgagg cattaccagg tcaccttcgt cgggttgtca
     3361 aaaccccggc ctcttcgccg ggatcatcga gaagatccca tatctcaagg agctgggcat
     3421 caccgccatt gaactgcttc cggtcttcga ctttgacgag aacgaggtca aacagctcaa
     3481 tcctgttgat ggcacacccc tgccgaacta ctggggatac gatcccttca gctttttcgc
     3541 tcttcagagc tggtactgta agtctcctga agctggcaac cattacactg agtttcggga
     3601 tatggtcaag gcgttacata aagcagggat agaagtgatc cttgatgtgg tcttcaacca
     3661 caccggcgaa ggcgatcaca ggggccctac catcagcttc agaggcttcg ccaatagcac
     3721 ttactacctg ctctcgcagc aggacaggca atactacatg aactactccg gttgtggcaa
     3781 caccatcaac ggcaatcatc ccatcacgga aaagctcatc gtcgataccc tgaatttctg
     3841 ggtgaaagaa atgcacgtgg acggtttccg cttcgatgag gccgtcattt tgtgtcgcga
     3901 cgaaaatggg ctgcccatgc tctatccacc agtcatctgg catattgaac tggaggactc
     3961 cctggctgac accaaagtca ttgccgaggc ctgggatgcc gccgggctct accaggtcgg
     4021 ctacttccct ggttatcgtt gggcagaatg gaacgggcgg taccgcgaca ccattcgacg
     4081 ctttgtcagg ggcgatcggg gatactttga tggaaaaacc atggttggcc gagttgccga
     4141 tatcatcgcg ggaagtgcca gcatcttcga atccagcggc gaactgccaa tcaacagcgt
     4201 caatttcatc accgcccacg atggatttac gctcaatgac ctggtgtcgt acaacaataa
     4261 acacaacgaa gccaacggcg aaggcaatcg ggacggcacc gacaataatt tgagctggaa
     4321 ttgcggcgtt gaaggggaga ccgacgatcc tgatattgag gcattgcgca agcgtcagat
     4381 caagaatttt gcggctattc ttctgctctc ccaggggatt cccatgtttg tggccggcga
     4441 tgaaatcaga cgaacacaaa agggaaacaa caacgcctac tgccaggata acgagatcag
     4501 ttggttcgat tggaatcttg cgaaaaagaa ccaggagatg ctccgcttct tcaaacagat
     4561 gattgcattg cgcaaaagcc atcctctcct gcaacgcagt cgcttcttta tgggtcaact
     4621 gaatcagcgt ggactggccg acatctcctg gcatgggtgc cgcttgaata gtccgggctg
     4681 gtacgatccc aattcctcgg tccttggctt cacgatgggc ggctttgacg gtgatgtcga
     4741 tctccacgtc atgctcaata tggactggca gaacctcgat tttgacattc tctccattga
     4801 ggggaggaaa tggtacaggg cagtagacac cgccctgcct tccccagagg acatcgccga
     4861 agcgggacaa gaggtagaga ttttcggcaa cacctaccac gtgaacggtc gtagcgtggt
     4921 cgttctcctt tccaggtaag gtagcttatt tacagagaac acaagaggag atttcaggct
     4981 catatcacac gaagtgtgcc gtcccaggaa aaaaggagaa cagcatggac aaattgagtt
     5041 ggacgtcttc ggacaccatt gccggctacg ttacctcttt cgagagggac aggaaaacgt
     5101 tcggcttgag gacaaacaat gaccgcgagt ttcgggtgaa aataaccgat gccacctacg
     5161 cagaactggt gcaaaacctg gatgagcctt tccaggaccc caggacacca ctagaaagca
     5221 taatcacgcc cggcaggtac ctgtttgcct gcggcatctt ctacccggag gctggtgagg
     5281 tcaagtttga ggccaagcgc atcatccttg tcggccgtaa cgcggatgaa tatcgctttg
     5341 aggtgcccga ctggtggatt cgacagatcc gctcgcttgc tgaattctac ttcaacgcgc
     5401 aattccccga tggcaccatc gattaccaca actatcgcac gcacctgaca ctggaggggc
     5461 aaaagcttcc cggtacgcgc caggagacag ataccatttc gcgcatgatc tatggttttg
     5521 cgacggcgta tatgctgacc ggcgtggatc gctacctgga ggcagcagag cgtgggacgg
     5581 agtatctgcg cgagcatatg cgtgccttcg atgaggacag ggattgtgtg tactggtatc
     5641 acgggatgga tgtcatcggc aaccgcgagc gaaagatctt cgcctcgcaa ttcggtgacg
     5701 acttcgatgc catcccggcc tacgagcaga tctatgccct ggttggtccc atccagacgt
     5761 accgcgtgac gggcgacgcg cgcatcttgc aggatgcaga gaagacaatt aacctgttca
     5821 atcagttctt ccttgatcgg gaaaagggcg gttacttcac gcacatcgat ccggtgaact
     5881 tcgatccgcg cgccgagtcg ctggctgcca atcgtgcgcg taaatgctgg aactctgtgg
     5941 gtgatcacgc tccagcctac ttgattaatc tgtggctggc gacgggtgat aagcgcttcg
     6001 aggatttcct ggtcaatatc gccgatacga ttgtcgagca cttccctgat tatgagcaca
     6061 gtccctttgt gcaagagcgc ttccacgagg actggagcca tgatacgacc tggggatggc
     6121 agcagaaccg ggcggtggtc gggcacaatc taaagatcgc ctggaatctg atgcgcatcc
     6181 atcatttgcg gcccaatgag cggtacaccg aactggcgcg caagatcgcg gcgatcatgc
     6241 caacagtggg tatggatcgg cagcgcggcg gctggtacga tacgatggag cgcgtgcgga
     6301 gcgcaggcga ggaatggcat cgctttgtgt ggcatgatcg caaggcctgg tggcagcagg
     6361 aacaggcgat tctggcgtac ctgatcctgt atggttcgct tagacagccg gaatatcgac
     6421 aactggcccg cgagtcgatg gcgttctaca acacgttctt ccccgatcac gataacggcg
     6481 gcgtatactt caacgtgcag gcgacgggca ttcctttcct gctagggacc gaacgcttca
     6541 aggggagcca ttcgatgtcg ggctatcact ccttcgagct atgctacctg gcggcagtct
     6601 attctaacct gcttattaac aagcagccga tgaatctcta cttcaagccc aaagcagggg
     6661 ccttcaagga taatttgctg agggtcatgc cagatatcct gccgccgggg agcgttcgga
     6721 tccagcaggt gtggctcaac ggcgatcgat accaggactt cgatccggtg gcggggacgg
     6781 tgacgttgcc ctcgatgcag ccccagcaat ccctggcaca gcgtccatcc tgggcgggta
     6841 gcccctaccc catgctaacg acgatgcgtg aactgaaggt gcgtgtgcgt ctcgccccga
     6901 ctggcacctt gttcgacgcg ctgctcgatc tgagtgatga ggtcggccag ctcgtcctgt
     6961 atggcgatct cagcgaagtc gccgcgccag ccttcaaagc ccatatggaa aaaattgtgg
     7021 ctgctcaacc caggcgcgtc gtgttgcgcc tggaagattt gcaaacaatc gcggaggttc
     7081 ctgcccgcat gttgtgcttc ttcagtgata agttcacgac caacgaggcg atctacgtgg
     7141 tgggcacgaa tgagaaggtg aaagaaaccc tccagcgtgt cggggtctgg gaagagctca
     7201 atgcgatgga aacgtatgac gagctatggc tgagatcctg accaaattgt gcagtaagcc
     7261 cgcattacga tcaaagtgtg cactctctat gttggccaat ttccaacagc aatgaggcca
     7321 tggtctattt caccatgctt cggttcatgc tcactcgctt tgccagggtt tgttcagtgc
     7381 acgtgacggg atggtacctt ctttatccta tggaccacat tagctcttct ttgatgcctc
     7441 tctcgctccg gcctgctct
//
LOCUS       DASZAK010000258         2714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_255652, whole genome shotgun sequence.
ACCESSION   DASZAK010000258 DASZAK010000000
VERSION     DASZAK010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255652"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2683
                     /locus_tag="VGT44_04460"
     CDS             <1..2683
                     /locus_tag="VGT44_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2580086.1"
                     /translation="THPSIVRILDYGVQDGVPFLVMDYAPNGSLRQRYPKGSVLPLSQ
                     IVSYVKQVAAALQHAHERKFIHRDVKPENMLVGRQQEVLLSDFGLTTLAHSSSSLSAK
                     EAVGTLPYIAPEQIEGHPRAASDQYALGMVIYEWLCGQSHFDGSMSEVMVQHLSIAPP
                     PLRERVPTISPEIEQVVLQALAKDPKDRFASVQDFAIALEQASQLVPSSVALLPRDQT
                     DQVQPATLDYTTAAVALSHHTTLMEATPSTEPLALPTQPAMSPGPSQLPEESVIPQSQ
                     VMDEPTPPRQPGEPTAPAAPLPTIVPHPPKPMSTRQQRGISLTTTGLLVALVVLVIAA
                     GVLGSILLTHFGGLGAHSGASTLTPIRGGTWTDDLYGEPDSLIPNGSTSGVTYSTMLD
                     QALYLPLFYGDAQGVIHPGAASEVPTVQNGGISADYKTWTFHLRPHLIWSDGQPYDAR
                     DVDYTWQLWHNPRFAGGSLPGLNLITGADVSADYLSITFHFQQAFAPFLSYWVDGLYA
                     PLPAHHFNAMAPEQILKSDENRNPKVTSGPFMMRESVPGNHYTLVRNPRYYRASEGLP
                     YLDKVVFHIVADEETILKDLQAGTITSAWFLDVRKVQEYQRLTHYTLISTPTSSTFEA
                     MYFNFHNVVLASHREVRQAIAMAIDQQALIQVARHGFAMPLCTDHPSAYHPGYEPFPN
                     CPVFDPLAANKLLDDNGWVKGPDGVRAKNGQRLEFEYSTSLNQPWRVDGEAIIKRNLQ
                     AIGIKLDIQNYPFDTFFNSFLPQGNASPPTGAVAGRYDIAEFENSPGTTYDPDDSSSL
                     ACDQVPPNGYNVDFYCNRALDALYKKEQTTADPNMRQQIFDQIHKIYLTDHPFITLYS
                     PAYIAMVRKGMHNYLPGPLGASETINIWEWRCDQGKC"
BASE COUNT          571 a          855 c          739 g          549 t
ORIGIN      
        1 gacgcacccc tcgattgtgc gtattctgga ttacggcgtg caggacggcg tacccttcct
       61 ggtgatggat tatgcgccca acggctcctt gcgccagcgc tatcccaagg gcagtgtact
      121 gcccttgtcg cagatcgtct cttatgtcaa gcaggtggcc gctgctttgc agcatgctca
      181 tgagcgcaag ttcatccacc gcgatgtcaa gccggagaac atgctcgtgg gacgacagca
      241 ggaagtgctg ctctccgatt ttggcctgac caccctggcg catagcagct cctcgctcag
      301 tgctaaggag gcggtgggca cccttcccta tatcgcccct gaacagattg aaggacaccc
      361 ccgcgcggcc agcgaccagt atgccctggg gatggtgatc tacgaatggc tttgcggcca
      421 gagtcacttt gatggctcga tgagcgaggt gatggtccag catttgagca tagcaccacc
      481 gccgctgcgg gagcgtgtgc ctaccatctc acccgagatt gaacaggtgg tactgcaagc
      541 cctggccaaa gaccccaaag accgctttgc ctcggtgcag gattttgcga ttgccttaga
      601 gcaagccagc cagcttgtcc catcctctgt ggccctactt cccagggatc agacggacca
      661 ggtgcagcct gctacgctcg actataccac tgccgctgtt gcgctcagtc atcacaccac
      721 gctaatggag gcgaccccat ccactgaacc gttagcgctg cccacacaac cggccatgtc
      781 accgggcccc tcgcagttgc cagaggaatc agttatacca cagagccagg tcatggacga
      841 gcctactcca ccgcgccagc caggagaacc aacggctccg gcggctccct tgcctactat
      901 cgtgccacat cctcccaagc caatgagtac ccgtcagcag cggggcatct ctcttactac
      961 caccgggctg ctcgttgcac tcgtggtgct ggtcatcgct gctggggtcc tgggcagcat
     1021 cctgctcacg cacttcgggg ggctcggcgc acacagtggt gcctccacgc tcactccaat
     1081 acgcggaggc acctggacgg acgatttata cggagagcca gattcgctca ttcccaatgg
     1141 ctcgacctcg ggggtaacgt attcgacaat gctcgaccag gcgctgtatc taccgctttt
     1201 ctacggggat gcgcaagggg tgattcaccc tggagcggcg agcgaggtgc ccacggtgca
     1261 gaatggaggg atcagcgctg actacaaaac ctggacgttc catctgcgac cccacctgat
     1321 ctggtcggat ggacaaccct acgatgcccg cgacgtggac tacacctggc agctctggca
     1381 caatcccagg ttcgccggcg gcagtctccc aggcctgaat ctgatcacgg gggcagacgt
     1441 ctccgcggac tatctctcga tcaccttcca cttccagcaa gccttcgctc ccttcctctc
     1501 atactgggta gatggcttgt atgccccgct gccagcgcac catttcaacg cgatggctcc
     1561 tgagcagatt ctcaagtcag atgagaaccg gaaccccaaa gtcacaagtg gtcctttcat
     1621 gatgcgtgag agcgtgccag ggaatcacta cacgctggtg cgcaaccccc gctattaccg
     1681 ggccagcgaa gggctgccct acctggacaa ggtcgtcttc cacatcgtcg ctgatgagga
     1741 gaccatcctc aaggatctgc aggcgggaac gatcacttca gcctggttcc tggatgtgcg
     1801 caaggtgcag gagtatcagc gcctcaccca ctatacgctc atctctactc ccacgagtag
     1861 caccttcgaa gcgatgtact tcaacttcca caacgtggta ctggccagcc accgggaagt
     1921 gcggcaggcc atagcgatgg ccattgatca gcaggccttg atccaggtgg cgcgccatgg
     1981 gtttgccatg ccgctgtgca ccgaccatcc atcagcctat catcctggct atgagccatt
     2041 tcccaactgt ccggtatttg atccgctggc cgccaacaaa ctgctggacg ataacggctg
     2101 ggtaaaaggc cctgatgggg tgcgggccaa gaatgggcag cgcctggagt tcgagtactc
     2161 gacgtcgcta aaccaaccct ggcgcgtgga tggcgaagcg atcatcaagc gcaatttgca
     2221 ggcgattggc atcaagctcg acatccagaa ctatccattc gatacgttct ttaattcctt
     2281 cttgccccaa ggaaatgcat cgccacccac gggagccgtg gcgggcaggt atgacattgc
     2341 cgagtttgag aacagccctg gcaccaccta tgaccctgac gattcctcgt cactggcgtg
     2401 cgatcaggtg ccgccgaatg gatacaacgt tgacttctac tgcaaccgtg ccctggatgc
     2461 cctctacaaa aaggagcaga cgacagcgga tccaaacatg cggcagcaga tcttcgacca
     2521 gatacataaa atctacctga cggaccatcc cttcattact ctctacagcc ctgcatacat
     2581 agcgatggtg cgcaagggga tgcacaatta cctgccaggg cccctgggcg catcggagac
     2641 catcaacatc tgggagtggc ggtgcgatca ggggaagtgt taggctgagc tcgcgcctcc
     2701 tccttttagg actt
//
LOCUS       DASZAK010000259         4093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_326328, whole genome shotgun sequence.
ACCESSION   DASZAK010000259 DASZAK010000000
VERSION     DASZAK010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326328"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..2231)
                     /locus_tag="VGT44_04465"
     CDS             complement(198..2231)
                     /locus_tag="VGT44_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580087.1"
                     /translation="MEVPEHDHQHRMTVSEQLTLSVLWFSLNFQNAALLPIVIPTEIL
                     LFINGQVGNAQQATLLGFISTGGAIMTLFVPPVIGMLSDHTRASWGRRRPYILTGGLC
                     MFISGLVLGFAANIWFFLLGLILFQLSINAGMAGYQSLIPDQVPQEQRGTASAYLGLM
                     TILGNVCSLAIAAILLGSINASAPDLSLIRRGSIIYYILSGIAMLIGTLITVFGVHEV
                     PLPSRAISRQEKQPFQLRTWTRENWLKPWRERNFSWVFLTRFFVMMGLTLFMTFIEFY
                     FADVAHVQNFVQTTANVAILALLGAVFSAFTLGVLSDKTGRVGIVSLATTFMALAALA
                     FVLFPGVFALWPLGLLFGLGYGAYTSVDWALTLDSLPSADSVGKDLGIWNVSTTLPAI
                     IAPLVGGLIILVANHFGQRELGYRAVFALATFVLLLGAVFVLKVREKRAPATERTARA
                     ATITSTEAPRSVQDAAKPGRPREQRRSIGFGWKLAFRTRAGRARGFMHFWPFWEWFTL
                     TIWRVKSIPQSPYGLLMVHFVTYKGKPIDLPDGTHVAKGDRVAELHFRNRALLQAAQQ
                     TTPFGLLHMIADDMRALASWSQQPDFPTNLRAYYAVTLLSRPSRRLGFILRDRPWSLL
                     GWFDRIFMTGLLILYHQNGLDRLLQGTTYGSYPQELWMSRGEMTRRYGSHDEH"
     gene            complement(2323..3495)
                     /locus_tag="VGT44_04470"
     CDS             complement(2323..3495)
                     /locus_tag="VGT44_04470"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV2580088.1"
                     /translation="MTLHISREQARHLLAHYHFTPTDLPGVFERLGTVQYDPLNPVGR
                     NPDLVLQARVPGYQVDDWQQSAYTDRIIYDAWDKMACLVPISDWPMRAIIRQKLRPYH
                     DREILQNDPEGMDFVLATIDKKGPLSSLEFEDRTRVGEGSWYGDTRVKRLLRSLWACG
                     LLVTHHRKAGRHYYDRPERVIPARYFELEPLIDEEAYYRWIVMKRFQASGIMRLGGEA
                     SIWVACGDALQRKAALAHLVESGLLTPLYIGDLKNLYYMPTTALDLLDKAPLPPRMIF
                     LGPLDSILWDRKTIQHLFDFDYIWEVYKPAEQRKWGYYILPVFYGDRFVARAEMRLEQ
                     GTLVIARWWWEHDITPDVGILDALRSALEAFAKYAHATTIVAQDEVDCVVRNSISA"
     gene            complement(3492..3977)
                     /locus_tag="VGT44_04475"
     CDS             complement(3492..3977)
                     /locus_tag="VGT44_04475"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV2580089.1"
                     /translation="MNKPELLASMESSFNEMEQALSPLSETQWTTPGANGDWSVKDIV
                     AHLTAWQHVMLRRLHAAARHETPARELQTEEKDIDDMNQRFYVENRDRPTPVVYDDFR
                     ASYHDIVAQIQSLPDADLFEVGRFDWTQESALWQYVAGDTFEHYDEHLPSIRDWVATH
                     S"
BASE COUNT          851 a         1105 c         1348 g          789 t
ORIGIN      
        1 cctgtaatgc gggttcgctg gtcactcaga aggcggcgat ttatcttcga cgaaagatgg
       61 ctgctgttgg cgagaaaata gccgaattgg agcgagtgca agcctacctc aacagcaaat
      121 tggcagaaat gcttcaggaa gaacatgaac cggctctgca gaagcctggg cggtgataga
      181 tgcgggtcgc ttctccttca gtgttcatca tggctaccgt acctacgcgt catctcgccg
      241 cgcgacatcc ataactcctg ggggtaggag ccataggtgg tgccttgcag caggcgatcc
      301 aggccgttct ggtgatagag gatgagcagg ccggtcatga agatgcggtc gaaccagcca
      361 agtaggctcc aaggtcgatc gcgcaggatg aagcccagac ggcgcgaggg tcggctgagc
      421 agcgtgacgg catagtaagc gcgcaggttt gtagggaagt cgggctgctg cgaccagctt
      481 gccagggcgc gcatatcgtc ggcgatcatg tgaagcagcc cgaaaggagt ggtttgttgg
      541 gctgcctgga gtaaagcgcg gttgcggaag tgcagttcgg caacgcggtc gcctttcgcg
      601 acatgcgtac cgtcgggcag gtcgatgggc ttgcccttgt aggtgacgaa atgcaccatt
      661 agcaggccat aaggcgattg ggggatcgac ttgacgcgcc agatggtcag cgtaaaccac
      721 tcccagaagg gccagaagtg catgaagccg cgcgcacggc ccgcgcgtgt gcggaaagcc
      781 agcttccagc cgaagccgat ggaacgccgc tgctcgcgtg gcctgccagg tttagccgcg
      841 tcctggacac tcctgggggc ttccgtggaa gtaatggtgg cggccctggc cgttcgctcc
      901 gtggcaggcg cgcgtttctc gcgtaccttc agcacgaaga cagcgccgag cagcagcacg
      961 aaggtggcga gggcgaagac ggcgcggtag cccaactctc tttgtccgaa gtgattggcg
     1021 accaggatga tgagaccgcc cacgagtggc gcgatgatgg cgggcagcgt ggtcgagacg
     1081 ttccagatgc ccaggtcttt gccgacgcta tcggctgagg gcagcgagtc gagcgtcaag
     1141 gcccagtcta cgctggtgta cgcgccgtag cccaggccga agaggaggcc cagcggccac
     1201 aacgcaaaga cgccggggaa gagcacgaag gccagggcag cgagcgccat gaaggtcgtg
     1261 gcgagggaga cgataccgac acgcccggtt ttatcggaca gcacccccag ggtgaaggcg
     1321 ctgaagactg cccccagcag ggcgaggatg gccacgttgg cggtggtttg cacgaaattc
     1381 tgcacgtgcg caacatcggc gaagtagaat tcgatgaagg tcatgaaaag cgtcagaccc
     1441 atcatgacga agaagcgggt caggaagacc catgagaaat tgcgctcgcg ccagggtttc
     1501 agccagtttt cgcgcgtcca ggttcgcaac tggaatggct gcttttcctg gcgcgatatg
     1561 gcgcgcgagg gcagcggtac ctcgtgaacg ccaaagacgg tgatgagcgt gccaatcagc
     1621 atggcgatgc cgctcaggat atagtagatg atcgatcccc ggcgaataag gctcaggtcg
     1681 ggcgcgctgg cattgatgct gccgagcaga atagcggcga tggccaggct gcacacgttg
     1741 cccagaatgg tcatcaaccc caggtaggcg gaggccgtgc cgcgctgctc ctgcggcacc
     1801 tggtcaggga tgaggctttg atagcccgcc atgccggcgt tgatagagag ctggaagagg
     1861 atcagcccca gtaggaaaaa ccagatattc gcagcgaagc ccaacaccag gccgctgata
     1921 aacatgcaca gcccgcccgt gagaatatag ggcctgcgcc tgccccacga ggcgcgcgtg
     1981 tgatcggaca gcataccgat cacgggcggc acgaaaaggg tcatgatggc gccgccagtc
     2041 gagatgaagc cgagcagcgt ggcctgctgc gcgttgccga cctgcccatt gatgaagagc
     2101 aggatttcag tggggattac gattggcagg agggcggcat tctgaaagtt gagggagaac
     2161 cataagacgc taagggtgag ttgctcactg acggtcatac gatgttgatg gtcgtgttca
     2221 ggtacttcca taggctcgat ccatactttc cgccggggga aaacagcata cgatatgttc
     2281 aaaattgctc tgttgcacat tataacatga tgagaggatg atttatgccg agatgctgtt
     2341 cctgaccacg caatccactt cgtcctgcgc gacgatggta gtcgcatggg cgtattttgc
     2401 gaatgcctcc aaagctgacc gcagcgcgtc cagtatgccg acatcgggcg tgatgtcgtg
     2461 ttcccaccac cagcgcgcaa tgacgagtgt gccttgctcc agacgcatct cggcgcgggc
     2521 gacgaagcgg tcgccgtaga agacgggcag gatgtagtag ccccatttgc gctgctcggc
     2581 gggtttgtag acttcccaga tgtagtcgaa atcaaagagg tgctggatgg ttttgcgatc
     2641 ccagaggatg ctgtcgagcg gacccaggaa gatcatgcgc ggcggcagtg gcgctttgtc
     2701 tagcaggtcg agggcggtgg taggcatgta gtagaggttt ttgaggtcgc caatatacag
     2761 gggagtcagc aagccggact cgaccaggtg cgccagggct gctttgcgct ggagggcatc
     2821 gccgcaggcg acccagatcg aagcctcgcc gccgaggcgc atgatgccgc tggcctggaa
     2881 gcgtttcatc acgatccagc ggtagtaggc ttcttcgtcg atgagcggct ccagttcaaa
     2941 gtagcgcgcc gggatgacac gttctggccg gtcgtaatag tgccgcccgg ccttgcgatg
     3001 atgcgtgacg agcagaccgc aggcccacag cgagcgcagc aatcgcttga cgcgcgtatc
     3061 gccataccac gagccttccc caacgcgcgt gcgatcctca aattccagcg aggagagcgg
     3121 tcccttctta tcgatggtgg ccagcacgaa gtccatacct tcggggtcgt tctgcaaaat
     3181 ttcgcggtcg tggtacgggc gaagtttctg gcgaatgatg gcgcgcatcg gccagtcgct
     3241 gatcggcacg aggcaggcca tcttatccca ggcatcgtag atgatgcggt cggtgtaagc
     3301 cgactgttgc cagtcatcga cctggtagcc cggcacacgc gcctgcaaga ccaggtcggg
     3361 attgcgcccg acggggttga gcgggtcgta ctgcaccgtc cccaggcgct cgaagacgcc
     3421 gggcagatcg gtgggggtga aatgatagtg cgctagcagg tgacgggcct gctcgcgaga
     3481 gatgtgcagt gtcatgaatg cgttgctacc caatctcgta tcgacggtaa atgctcatcg
     3541 taatgttcga aggtatcgcc cgcgacatac tgccataacg cgctttcctg tgtccagtcg
     3601 aagcgaccga cttcgaacag gtcggcatcg ggcagggatt ggatttgcgc gacgatgtca
     3661 tgatagctgg cgcgaaaatc gtcatatact acgggtgttg gccggtcgcg gttctcgacg
     3721 tagaagcgct ggttcatatc gtcgatgtct ttctcctccg tctgcagttc gcgcgcgggc
     3781 gtttcgtggc gagctgcggc atgcaggcgg cgcagcatga cgtgctgcca cgcggtgagg
     3841 tgcgccacga tatccttgac ggaccagtcg ccattcgcgc cgggggtcgt ccattgtgtc
     3901 tcgctgagtg gcgatagggc ctgttccatc tcgttgaagc tgctttccat tgaagcgagc
     3961 agttcgggtt tgttcatggt tgcctccgat attaatgaac gttcaccatc aagatcggta
     4021 ataagctgtc atcctgagcg cagcgaagga tctcactcgc cgaccactga gatccttcgc
     4081 tgcgctcagg atg
//
LOCUS       DASZAK010000260         1862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_290022, whole genome shotgun sequence.
ACCESSION   DASZAK010000260 DASZAK010000000
VERSION     DASZAK010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290022"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1862
                     /locus_tag="VGT44_04480"
     CDS             <1..>1862
                     /locus_tag="VGT44_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEV2580090.1"
                     /translation="ALPTTIIPAVKSQQRQPTSALQPGQPVYASTAYARRLQRHQRAR
                     EKKLSWQDLRQFSQFITPFKWQITTALLLTIGVGLTALPLPFIFHIMIDTVFQQRNVP
                     LFIWSLVFLLAVQWVDELLSFLNRNVQGSLSRATNLNITHRFYKHMLRLPLAFYQQFQ
                     GTGQVLSRLNEVTSAQSTVIQVVMDTVVNSILLIIYTVVLFLSDWRLTLVVLAVSPFY
                     VGVSLYFNRRVRQLSRQVLESYAAMNGAMYEGLTGIKTVKALAAEHRFGRKIKQLIMV
                     TNQLSFRRTMFQSRANLVIGIVQALCVIAILSFGGYLVLSGQLSAGILAAYILILRQL
                     STPVATLNGVNQQIQAAAVAIDRLFEVLNYPEEADAERGIALAQARGEMTFEHVHFSY
                     TPGIEVLHDISFHVKAGSTIAIVGRSGAGKTTISNLLMRFYQPDSGRITIDGYDLRDL
                     KIESLRSQLGVILQDESLFSGTIADNLTFGLLRRVSYEEIEEAARNANILDFIQSQPN
                     GLQTALHERGQSLSGGQRQRIAIARMFLHQPRILIMDEPSSALDNESESLIQQAFKRL
                     TAGRTTFIIAHRLSTIRNADHILVIDGGRVVEQGNHDMLLAQHGIYWHLYNSYG"
BASE COUNT          384 a          596 c          481 g          401 t
ORIGIN      
        1 cgcgctaccc actactatta ttccggcggt gaagagccag caacgccaac ccaccagcgc
       61 cttgcaaccc ggtcagccag tatacgccag cactgcctat gctcgcaggt tgcaacgaca
      121 tcagcgcgcg cgtgaaaaaa agctgagctg gcaggatttg cgccagttca gccagttcat
      181 caccccgttt aaatggcaaa tcactaccgc gttgctgctg accatcggcg ttggtttgac
      241 cgctttaccg ctacccttca tctttcatat catgattgat acggtattcc agcagcgcaa
      301 cgtcccgctt ttcatctggt cgctcgtttt cctgctggcc gtgcagtggg tcgatgaact
      361 gttgagcttt ctcaaccgca acgtgcaggg gtcattgagc cgcgcgacca acctcaacat
      421 cacgcatcgc ttctataagc acatgctgcg cctgcccctt gctttctacc agcaattcca
      481 gggaactggc caggttttaa gccgcttgaa cgaggtcact tcggcgcaat cgacggtcat
      541 tcaggtggtg atggatacgg tggtcaattc gatcctgctc atcatctaca cggtggtgct
      601 tttcctatca gactggcgct tgaccctggt ggtgctggcc gtctcgcctt tctatgtggg
      661 cgtcagcctc tacttcaatc ggcgcgtgcg ccagttgagc cgccaggtcc tggagagcta
      721 cgccgccatg aacggcgcga tgtacgaggg cttgaccggc atcaagacgg tcaaggcgct
      781 ggctgccgaa catcgctttg gtcgcaagat caagcagctc atcatggtga caaatcaact
      841 ttcattccgg cgcacaatgt ttcaatcgcg ggccaacctg gtcatcggca tcgtgcaggc
      901 gctgtgtgtc atcgccatcc tctccttcgg cggctacctg gtcttatccg gtcagctctc
      961 cgccggaatt ctggcggcct acatcctgat tttgcgccag ctttcaaccc ccgtcgccac
     1021 attgaacggt gttaatcaac agatacaggc ggcggcagtc gccattgacc gcctcttcga
     1081 ggtgctgaat taccccgaag aggccgacgc ggaacggggc atagcgctgg cccaggcgcg
     1141 aggcgagatg acctttgaac acgtgcattt ctcgtatacg cccggcatcg aagtgctgca
     1201 cgatatctca tttcatgtca aggccgggtc taccatcgcc atcgtcgggc gcagtggcgc
     1261 aggcaaaacc accatctcca acctgctgat gcgcttctac cagcccgaca gcgggcgtat
     1321 tactatcgat gggtatgacc tgcgggacct caaaatcgaa agcctgcgca gccagctcgg
     1381 cgtgatcctg caagacgaat cacttttcag cggcacaatc gccgacaacc tcacctttgg
     1441 tctgctgcgt cgcgtctcct acgaagaaat tgaagaggcc gcgcgcaacg ccaatatcct
     1501 cgatttcatc cagtcgcagc ccaacggcct gcaaacagcc ctgcacgaac gcggccagag
     1561 cctttccggc ggtcagcgcc agcgcatcgc tatcgcgcgc atgttcctgc accagccgcg
     1621 cattctgatt atggacgagc catcgtccgc cctcgataac gagtcagagt cgctcatcca
     1681 gcaggcgttc aagcgcctga ccgccgggcg cacaacattc attatcgccc accgtctcag
     1741 caccatccgc aatgccgatc acatactcgt catcgacggc ggtagagtcg ttgagcaggg
     1801 aaaccacgat atgctgctgg cgcagcacgg catctactgg cacctctaca acagctacgg
     1861 ca
//
LOCUS       DASZAK010000261         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_539208, whole genome shotgun sequence.
ACCESSION   DASZAK010000261 DASZAK010000000
VERSION     DASZAK010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539208"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGT44_04485"
     CDS             <1..192
                     /locus_tag="VGT44_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580091.1"
                     /translation="ILYCVVRPVNERETRTLVAGPASRSRSRQRFLVKHWRRTETTYR
                     RIAGDDHPHDQLSPMKPHH"
     gene            complement(189..527)
                     /locus_tag="VGT44_04490"
     CDS             complement(189..527)
                     /locus_tag="VGT44_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580092.1"
                     /translation="MRHSARDLANLADGLTGVQIAEAFLTAASPGVIALFLENQYYPT
                     HEAYLSALAEAMKEEYDAIVGAGFLLQLDCPDLGVSRVRGEDWREDYRVLHIQALNQA
                     VPTRVSKVPD"
     gene            complement(666..839)
                     /locus_tag="VGT44_04495"
                     /pseudo
     CDS             complement(666..839)
                     /locus_tag="VGT44_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1161..1607)
                     /locus_tag="VGT44_04500"
     CDS             complement(1161..1607)
                     /locus_tag="VGT44_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV2580093.1"
                     /translation="MTVEETRTNAEAEIKRVIEDGVEAIRDKNIEGIMALYAPEVVSF
                     DIVPPLQYVGAEAFRKAWEAVFSSFQGPIGYDIHDLGITVGEDVAFSHSLNRISGTLN
                     TGQKTDLWLRWTAGFRKINGKWLVVHHQNSVPVDLQTGRAVLDLKP"
     gene            complement(1631..>1936)
                     /locus_tag="VGT44_04505"
     CDS             complement(1631..>1936)
                     /locus_tag="VGT44_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2580094.1"
                     /translation="DAASALVAAVTQPVPSGVYDIVDDEPLTHREVFAAMAQAVGRQH
                     LWRPPAVLMRMMTGVVYEIITRSLRISNRRFKAVSKWNPSVPNARVGWSRLGEESKS"
BASE COUNT          361 a          601 c          545 g          429 t
ORIGIN      
        1 attctatact gtgtcgtccg tcctgtcaac gagcgagaga ccaggacgct tgtcgctggt
       61 ccagcgtcac gctcacgctc tcgccagcgt ttcctggtga aacactggcg acgaactgag
      121 acgacgtatc gacgcatcgc cggcgatgat cacccccacg accagctctc ccctatgaaa
      181 cctcaccact aatctgggac cttgctcact ctcgtgggta cggcctggtt gagcgcctgg
      241 atgtgcagga cgcggtaatc ctcgcgccag tcctcaccgc gtacccggct gacgccgagg
      301 tcgggacaat ccagttgcag gaggaatccg gccccgacga tggcgtcgta ttcctccttc
      361 atcgcctcag ccagggcgga gagatacgct tcgtgcgtcg gataatactg gttctccagg
      421 aacagcgcga tgacgcccgg cgaagcggcg gtcagaaagg cctctgctat ctgcacgcct
      481 gttaagccat cggccagatt ggcaagatcg cgggcggagt gtcgcatagg tgctgtagct
      541 gatcggcagg cctgggcaag cttccggtgt gcgtggtgag gattctctgc gtactgctcc
      601 aattttcaga tgccaatgta tgcaagcacg cacccccaga gaatttcgtt gttgctgctt
      661 cacctcaagg tggccagccg gttcagcccc acatgggtgc cgcatgctac cggttttttc
      721 accgggacct cgtcaggcat ttcgatctgg atggagacaa accaccactc cgctgttctc
      781 gtgatccgtg cgcctgttac tttcccgttg aagcgcggat tctcggccat gctcacgaaa
      841 ctcggaaaat tgatcgcctg ccccgatgta cacaattggt cgcccacgcg agaggacatc
      901 aacggtacag gccattttaa ccaggagtgc ggattgcgat gcgctgcacg ggcgacgctc
      961 tggccgattt ggagagaaga tgctgatttc ttggccgtgc ttctgttgca agcctctccc
     1021 cttctttcgt cagtgcaagc cttatgcctg ccgcgtggca tctgcataaa gccgggggat
     1081 gcgccacctc cgctcgcccc atacggagga ggatgcgctt ctttggggaa gctcgctcct
     1141 cccctcgggc catgcaattg ctagggcttg aggtccagca ccgcgcggcc agtctgcaaa
     1201 tcgaccggca ccgagttctg atggtgcacg accaaccact tgccgttgat ctttcggaag
     1261 ccagcggtcc accgcagcca gagatcagtc ttctgcccag tgttcagggt tccgctgatc
     1321 cggttgaggc tgtggctgaa ggctacatcc tcgcccaccg tgatgccgag gtcgtgaatg
     1381 tcatagccga tcggaccctg gaacgacgag aagaccgctt cccaagcttt cctgaaggct
     1441 tctgctccca catattgcaa cgggggcaca atgtcgaacg acacgacctc cggtgcgtag
     1501 agagccatga tgccttcgat gttcttgtca cgaatcgcct cgaccccatc ctctattacc
     1561 cgtttgattt cggcttcagc gtttgtccta gtttcttcga ctgtcatagg ttcctcctta
     1621 ttctcgcttg ttatgatttg ctctcctctc ccagacgcga ccagcccaca cgggcgttgg
     1681 ggaccgacgg gttccatttg ctcactgctt tgaagcggcg attggaaatg cgcaagctgc
     1741 gggtgataat ctcatagacg acgccggtca tcatccgcat cagcaccgca ggagggcgcc
     1801 acaggtgctg gcgccccacg gcctgggcca tggccgcaaa gacctcccta tgtgtcagtg
     1861 gttcgtcatc aacgatgtcg tagacgcctg aggggaccgg ctgcgtcacc gcagcgacga
     1921 gcgcgctggc cgcatc
//
LOCUS       DASZAK010000262         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_326343, whole genome shotgun sequence.
ACCESSION   DASZAK010000262 DASZAK010000000
VERSION     DASZAK010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326343"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..1445
                     /locus_tag="VGT44_04510"
     CDS             69..1445
                     /locus_tag="VGT44_04510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F390 synthetase-related protein"
                     /protein_id="HEV2580095.1"
                     /translation="MSASRKLSLLRHMLMARWRWKHLRSAALERFQELHVQQIVAFAA
                     QHSPFYRSHWAGHALSEWRTLPCVDKALMMQHFDTFNTRGISREQAMAIALEAERSRD
                     FRPVLEGVTVGLSSGTSGHRGLFLANAWEQSAWAGTILARTLHHLPRHVLRVAFFLRS
                     NSNLYEGVASRLIQFRYFDLMLPLKAAVSALNEFQPHIIVGPPSLLGFLANEREGGRL
                     RARPGRLISVAEVLEPHDRQRLEAIFDAPVHQIYQCTEGLLAVACEQGSLHIQEDVVM
                     IQLEPLPGGDGAQEQRFMPVVTDMWRRTQPIIRYRLNDVLQMETQPCACGSAFRVIRA
                     IEGRCDDICYFESLAGGTRPFFPDTIRRMILLSSPHILDYQAIQERCGQLSIHLVVAP
                     LASFSEVAQTVQESVQATVAQYACRPALVRIEEGLEPAVPGVKRRRVHQTLRCAQADM
                     SGFGRETS"
     gene            1666..2085
                     /locus_tag="VGT44_04515"
     CDS             1666..2085
                     /locus_tag="VGT44_04515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEV2580096.1"
                     /translation="MLDVTTEWGQHAEQRLRSNIIAWLTTVGSDGRPYTSAVWFLWDG
                     KAILIFSQPDKQKVRNLRKNARVTLALDETQKGDDVVIVEGTAELLDDPDLSAVLPAY
                     IEKYGAIIQSMEWTPEAMAADYSQGIRVTPTKFRSWT"
BASE COUNT          433 a          588 c          645 g          459 t
ORIGIN      
        1 ctgtcccgac gtgacgattg tgccaagtca ctgccctgaa gcttttgcgc aggaagtcga
       61 ggcaactcat gtcggcatcg cgtaaactct cgctgctccg ccatatgctg atggcgcgct
      121 ggcgctggaa gcacctgcgc agcgcggcgc tggaacgctt tcaggaactg cacgtgcagc
      181 agattgtggc tttcgcggcg caacactcgc cgttctaccg cagccattgg gcaggtcatg
      241 cattaagcga atggcgcact ctgccatgtg ttgataaagc cctgatgatg cagcattttg
      301 acactttcaa tacaagaggc atttcgcgcg agcaggccat ggcgattgcg ctggaagcgg
      361 aacgcagccg cgattttcgc ccggtattgg agggcgtaac ggtcgggctg tcgtcgggga
      421 catcggggca tcgcggcctg ttcctcgcca acgcctggga acagagcgcc tgggctggta
      481 cgatcctggc gagaacgctg catcacctgc ctcgccatgt cctgcgggta gctttcttcc
      541 tgcgctccaa tagcaacctc tatgaggggg tcgccagccg actgatccag tttcgctact
      601 tcgacctgat gctgccactc aaagcagccg tttccgcgct caacgagttc cagccgcaca
      661 tcatcgtggg accgccttcg ttgctcggtt tcctggcaaa cgagcgcgag ggcggtcgcc
      721 tgcgcgccag acctggccgc cttatctctg tggcggaggt gctggagccg cacgaccggc
      781 agcggctgga agcaatcttc gacgcccccg ttcaccagat ataccagtgt accgaggggc
      841 tgctggctgt cgcttgtgaa caggggtcgc tgcatatcca ggaagatgtc gtcatgatcc
      901 agttggagcc gctgcccggt ggcgatggcg cgcaggagca gcgcttcatg ccggttgtca
      961 ccgatatgtg gcgcaggaca cagccgatta tccgctaccg tctcaacgat gtcctgcaaa
     1021 tggaaacgca gccgtgcgcc tgcggatcgg cgttccgcgt cattcgcgcc atcgaggggc
     1081 gctgcgacga catctgctat ttcgagtcgc ttgccggggg aacgcggcca tttttccccg
     1141 acacgatccg gcgcatgatc ctgctgtcaa gcccgcatat cctcgattac caggcgatac
     1201 aggaacgttg tgggcaactg agcatccatc tcgtcgtcgc tccactggct tcattcagcg
     1261 aggttgctca aactgtgcag gagagtgttc aggcaacggt ggcgcagtat gcctgtcgcc
     1321 cggccctggt gaggatcgag gaaggattgg agcctgctgt accgggggtc aaacggcggc
     1381 gggtgcatca gacccttcgc tgcgctcagg ctgacatgag cggctttggt cgcgaaactt
     1441 catgatcggc cagtcctgac ttgcggaacg acgaggattc gcgccgataa atcggctctg
     1501 agcgcggtaa accgtctcct acggggggtt cgatgctcgt ttacctataa gtcgttccta
     1561 caccccgtca atatggaata aagccgccat ctcaacgtgt aatgttacag agagtcacga
     1621 gcaaatgaag cgtttacctc tcgtttttca ggaaagggat tagttatgct tgatgtaacg
     1681 acagaatggg gacaacatgc cgagcagcgt ttgcgttcca atatcattgc ctggttgacg
     1741 actgtcggat cggatgggcg tccatatact tccgcagtct ggtttttgtg ggacggcaag
     1801 gccattctga ttttcagcca gccggataag cagaaagtgc gcaacctgcg caaaaatgcg
     1861 cgtgtcacgc tggcgctcga tgagacgcaa aagggagatg atgtggtcat tgttgaggga
     1921 acagccgagt tgctggatga tccagatctg agtgcggtgc tgcctgccta tatagaaaaa
     1981 tacggggcga tcatacaaag tatggaatgg acgcctgaag cgatggccgc cgactactcg
     2041 caggggatca gggtgacgcc aacgaaattt agaagctgga cataaccggg acaacgctgc
     2101 ttaggtatct ttgtggatac gtgat
//
LOCUS       DASZAK010000263         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_433378, whole genome shotgun sequence.
ACCESSION   DASZAK010000263 DASZAK010000000
VERSION     DASZAK010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433378"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VGT44_04520"
     CDS             <1..684
                     /locus_tag="VGT44_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580097.1"
                     /translation="YGQQVPPGYPPGYPAYPGYGQPGAPSVPLYGQPVPGGMPGYGQY
                     GQAAPPSQNRLPLLTPLPEVKRRPVWLIVLVAVLIVALVGVSVAAVVALSNSHNQPLQ
                     GPGSVGPTPTSTPNVVFFDPLTSNVNGWLTDSHCSFASDGYHVKANYLCFAPAGQFGD
                     ASISVKVQQINGDIHWFYGLVFRWASSGNYYLFNIDSNGKWEFDKVVNDQLTSIVPYR
                     AIGQKAPWL"
     gene            746..1996
                     /locus_tag="VGT44_04525"
     CDS             746..1996
                     /locus_tag="VGT44_04525"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2580098.1"
                     /translation="MGQQTVRKTFKYKLQPTPQQEETLAFVVRRCRELYNAGLQERRD
                     AWQKCGVSITAASQSAQVPESKDVRPEYREVHSQVLQDVLTRLDRAFQAFFRRVRAGE
                     TPGYPRFHGANRYSSFTYKQFGNGATLDKGFLVLSKIGRLAIRWSRPLEGTPKTVTIS
                     READGWYTCFSCEDVPIQPLPPTGQETGIDLGLESFATLADGSQIANPRILRVAERRL
                     KRAQRRVSRRTKGSHRRRKAVRLLTRAHQQVRRARQDFQHKTALALVRQYDTLYHEEL
                     QTANMVKNHSLAKSIADAGWSGFLNTLSFKAVCAGRRVVAVPPAYTSQACSGCGVMVQ
                     KGLSAPWHLCPDCGPRLHRDHNAALNILRLGKQRSGAGQAPQALPWATLAERSLSISR
                     MYAGECQSVRPHQRRPIDQEPSAI"
BASE COUNT          399 a          645 c          638 g          386 t
ORIGIN      
        1 tacggccagc aggtcccgcc ggggtaccct ccgggatatc cagcgtatcc gggctatggg
       61 cagccgggag ctcccagcgt gccactctat ggccagccag ttccaggcgg gatgccgggc
      121 tacggccaat atgggcaagc cgcgccgccc tcacaaaacc gcctgccgct gcttacgccg
      181 ctgcccgagg tcaaacgcag gcccgtctgg ctgatcgtgc tggtggccgt gctgatcgtg
      241 gcgctggtgg gcgtctcggt ggctgcggtg gttgcccttt ccaacagtca caatcaacca
      301 ctccaggggc ccgggtccgt aggcccaacg ccgacgtcca cgccgaacgt cgtcttcttc
      361 gacccgctga cctcaaatgt gaacggatgg ctcaccgata gccactgttc gttcgcgagc
      421 gacggctacc atgtgaaagc caactatctg tgcttcgcgc ccgctggcca attcggcgac
      481 gcgagcatca gcgtgaaggt gcagcagatc aacggtgaca tccactggtt ctacgggctc
      541 gtgttccggt gggctagctc tggcaactac tacctcttca acatcgacag caacggcaaa
      601 tgggagttcg ataaggtggt caatgaccag ctgactagca ttgtgcctta cagagcaata
      661 ggccagaaag ccccttggct ttagccaggg gatgaatggc gctcttgccg tgtagatgtg
      721 cgtctgccag acagaataga gtcctatggg ccagcagaca gttcgcaaga ccttcaagta
      781 taaactccag cccactcccc aacaggagga gacgctggca tttgtcgtgc gccgctgccg
      841 tgaactctac aatgctggcc tgcaagagcg ccgtgacgcc tggcagaaat gcggggtgag
      901 catcaccgcc gccagccaga gcgcccaggt gccagaaagc aaggacgtcc ggccagagta
      961 ccgtgaggtg cactcgcagg tgttgcaaga cgtgctgacg cgcctcgaca gagcctttca
     1021 ggcattcttc cgccgcgtca gggcggggga gacgccaggg tatccgcgct tccacggtgc
     1081 gaatcgttat agcagcttca cctacaagca gtttggcaac ggcgccacgc tcgacaaggg
     1141 ctttttggta ctgtccaaga tcggcaggct cgccatccgc tggagtcgcc cgctggaggg
     1201 cacgcccaag acggtcacga tctcgcgtga ggctgatggc tggtatacct gcttctcctg
     1261 cgaggatgtg ccgattcagc ccctcccgcc gacgggacag gagaccggca tcgacctcgg
     1321 cttggagtcg ttcgccaccc tggccgatgg cagccagatc gccaacccgc gcattctgcg
     1381 cgtggcggag cgtcgcttga aacgagcgca gcgtcgagtc tcgcgccgca caaagggtag
     1441 ccatcgccga cgcaaggcgg tcaggctgct gacacgcgcg catcagcagg tgcgccgggc
     1501 acggcaagac ttccagcaca agacggcact ggccttggtt cgccagtatg acaccctcta
     1561 tcatgaagag ttgcagacgg ccaacatggt caagaaccat tcccttgcaa agtctattgc
     1621 cgacgcgggg tggagtggtt tcttgaacac cctctccttc aaggcagtgt gtgccggtcg
     1681 gagagtcgta gctgtgccgc ctgcctatac ctcgcaagcc tgtagtggct gtggcgtcat
     1741 ggtgcagaaa ggcttgtccg ctccctggca tctgtgcccg gactgcgggc cacgcctcca
     1801 tcgagatcat aacgctgcgc tgaacatctt gcgcttgggt aaacaacgta gtggggccgg
     1861 gcaggcccct caggcgctac cgtgggccac tctcgccgag cgtagcctga gcatctcccg
     1921 catgtatgcg ggggagtgtc aaagtgttcg gcctcatcag cgaagaccta ttgatcagga
     1981 gccatcggct atatgaaagc agcggtcgcc cgcgtcctgg acgcgcttag ggctgcaggc
     2041 gtggcggcgc agatcgtcga gtttccac
//
LOCUS       DASZAK010000264         8730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_610877, whole genome shotgun sequence.
ACCESSION   DASZAK010000264 DASZAK010000000
VERSION     DASZAK010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8730
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610877"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VGT44_04530"
     CDS             complement(<1..949)
                     /locus_tag="VGT44_04530"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2580099.1"
                     /translation="MRRLFSFAPAMILILVLAACGSSGPTSSSSSSCPSSRSSLHFFV
                     ITHGQASDPFWSVVKKGVDQAAKDMCVTAIYESPTGATFDVVAMAHLIDTAVSAHPSG
                     LVVSIPDANALGPSIKAAVAAGIPVISINSGSDVAKSLGVLVHIGQTEEQAGIGGGEK
                     MGAAGVKHALCVNQEVGNAALELRCKGFKEGLAATGGTIKEIGVNLSNPTQTQQTIEA
                     ALQHDPTIDGILTLGPTGATPAIKALQDLNKLGQIKLATFDLSSDVLNAIKAGQMLFA
                     IDQQQYLQGYLPIVLLTLYATNLNTIANDILQTGPGFVTS"
     gene            complement(1109..3040)
                     /gene="iolD"
                     /locus_tag="VGT44_04535"
     CDS             complement(1109..3040)
                     /gene="iolD"
                     /locus_tag="VGT44_04535"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HEV2580100.1"
                     /translation="MNIRNHQTTRRLTMAQALIAFLKNQFVERDSVEQAFFAGCFGIF
                     GHGNLAGIGQALQQMPEFRYYQARNEQAMVHTATAFAKVHNRLRAFACTSSIGPGATN
                     MVTGAATATINRLPVLLLPGDIFARRNVAPVLQQLESEHSQDISVNDCFKPVSRYWDR
                     IMRPEQLLTALPEAMRVLTSPAETGAVTLALPQDIQAMAYDYPEAFFQRRLWRVARNR
                     PDRGALELAAAWIRASQRPLIVAGGGVIYSGATDLLRLVVELTGIPVGETMAGKGSLR
                     YDHPLNLGAIGATGTFAANRIAREADLVIGIGTRYSDFTTASKTAFQHPNVRFININV
                     ASFDAAKHHALSLVGDAQATLEELSALMEDYHVEESYRAQAAQLQAAWDAEVERIYAI
                     RHTPLPSQGELIGAVNELSAPDAVMVCAAGSLPGDLHKLWRARHPKNYHMEYGYSCMG
                     YEIAGGLGAKMAAPEREVYVMVGDGSYLMMNSEVVTSIQENYKLIIVLFDNHGFKSIG
                     SLSRSLGQDGFGTRYVYPRNGILPGDAMGSDAQTLSIDLAANARSLGALVIECNTYED
                     FVKALKQARTTGQTTVICIQNDRYEGVPGYESWWDVPVAEVSALPSVQAARQEWEAMR
                     AQERYYLEQQGPLSADARP"
     gene            complement(3061..4086)
                     /gene="iolC"
                     /locus_tag="VGT44_04540"
     CDS             complement(3061..4086)
                     /gene="iolC"
                     /locus_tag="VGT44_04540"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HEV2580101.1"
                     /translation="MDTTHKTYDIVAMGRSSIDLYSNDISAPFEEITSFAAYVGGCPT
                     NISVGMRRLGLRPVLLTAVGEDQVGNFILRFLREEGVETRYIPRKPGRRSSAVVLGIE
                     PPDRFPLTYYRDNCADIALTIDDALAAPLVDSRALLITGTGLSKEPSRSATLFAAETA
                     RQADVPVALDIDYRPDQWVDQRAFGVTLRSALRLVDIVIGTEDEIKAAMLTDVRQVSI
                     AHSQISDARVLGDTDAAIQRLLELGPRVLVQKRGEEGSRVHLTDGEARRIDVPGFPVE
                     VQNILGAGDAFASGFLYGFIKGWDWYRSARLGNACGAILVTKHGCANFMPTYDEVMAF
                     VQDHGGF"
     gene            complement(4115..5020)
                     /gene="iolB"
                     /locus_tag="VGT44_04545"
     CDS             complement(4115..5020)
                     /gene="iolB"
                     /locus_tag="VGT44_04545"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HEV2580102.1"
                     /translation="MRQYHADNLVVHPGNSSDPDVVIEVTPDLAGWEYVSFQVRRLAA
                     GRSWTFATGGYELAIVVLGGQLTVESEKDQWSRIGQRKHVFDGLPSALYFPRRTNLTV
                     RAESDCEFAVALAPTDQDREPRFVTPRDIEIEIRGGDNATRQINSIIPPGFPCDRLVV
                     VEVYTPGGNWSSYPPHKHDVHKTDQQHKLIEADLEEIYFYKFDKPEGFAFQRIYTVPE
                     SPLHRAGNPIDAALLARDNDVVLVPEGYHPVSGAPGYTTYYLNVLAGSAQSLANSEDP
                     RYAWVKQQYRSRDPRVPIYDIEQRG"
     gene            complement(5223..6254)
                     /gene="iolG"
                     /locus_tag="VGT44_04550"
     CDS             complement(5223..6254)
                     /gene="iolG"
                     /locus_tag="VGT44_04550"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEV2580103.1"
                     /translation="MPARVKIGVIGLGRMGRLYAQALATQVTGAQLYAVAEVGETTRA
                     QVESELNVPRSYADAYELIAHPDVQAVVIATPTSAHHDLAIAAAREGKAIFCEKPLAL
                     TLRETRSIIHAVESAKVPLQVGFMRRFDLAYQRAKAMIDDGQIGYPVTFKSLSRDPFC
                     PPRNYLDPAQSGGLIIDMAIHDIDLAHWLVGSGVERVSAEGTTLVCEELASVGDIDNA
                     LVNMRFASGALGNIEASRNAFYGYDIRTEVLGSKGAVMIGAHQYTPVVLLTRAGAKHD
                     VMPYLMERFGDAYRAQLQHFVDCLRAGETPSVGGAEAYAACKVGIAATQSYHTRRPVA
                     LSELDDAAL"
     gene            complement(6256..7314)
                     /locus_tag="VGT44_04555"
     CDS             complement(6256..7314)
                     /locus_tag="VGT44_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2580104.1"
                     /translation="MRQHEISIADIARVAGVSHTTVSRALRGNHLISLETRERIQRLA
                     QEMGYTPNAIAQSLQTRSTSTIGLVVTSIADPFLNDVVKGVEEVARAANFSVLLSATH
                     NDPEQEMVVIETFHRRRVDGILVASSRITSAYKQRLDHIRVPTVIINSQADSQNELLN
                     WVSVDDRFGACLAVEHLLQLGHRDIGYLGISSRPRSNRQRFQGYQGALAAAGIPFCEK
                     CILVSSGTEASHEEDVAVGQASLQHFLDNRVTAIFCYNDMAAVGLLMACRERGIDVPG
                     QLSVVGFDDIKLASYVSPALTTVRQPKLELGRLATEVMLDLLHNRPGKNHLLQPELIT
                     RASTALPVSVAGARKEFA"
     gene            complement(7494..8594)
                     /locus_tag="VGT44_04560"
     CDS             complement(7494..8594)
                     /locus_tag="VGT44_04560"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEV2580105.1"
                     /translation="MSVYSLASRRSRTGPPQVCVIAGDDAAPEVMRPTVELLRLLAPG
                     IRFVEALSGRAAIQQHSEAFPAATRRAIDDADCALFGASGGPSRPVLWYLRWGKQTFV
                     NVRPVRWSPGYRSPMLHPERIDYVIVRDNLEGIYPPREGDIAELSALPSSADWWQSPP
                     VESRGAYAVRVATDEQMQRVALAACELALQRQAGGYPGRVTLGAKYSILPRTDGRFRA
                     IVKEAVSAYPGLGYQEYLVDDLARRMVAEPETLDVVVLGNEHGDILADAASGCIGGLG
                     LSPSACYGEDYAYFEPVHGSAPDLAGKGIINPTAMLLTGALLLNYLGYHEEARRLERA
                     IRQTYQGGVALPVDQGGSATTMGFMEQVKTNL"
BASE COUNT         1749 a         2654 c         2593 g         1734 t
ORIGIN      
        1 ctgatgtaac aaagccgggg cctgtctgga ggatatcgtt cgcaatggta ttgagattcg
       61 ttgcgtacaa tgtcaacaag acgataggta gatacccctg gagatactgt tgctgatcaa
      121 tggcaaacag catttgacca gccttgatcg cgttcaggac atcggatgac agatcgaagg
      181 ttgccagctt gatctggccc agcttgttca ggtcctgtag tgctttgatg gccggggtag
      241 cacccgtggg gccaagtgtc aagataccat caatggtagg atcgtgctgt agagcggcct
      301 cgatggtctg ctgggtttgc gttgggttgc tcaggttgac accaatctct ttgattgtgc
      361 cgcctgtcgc tgccagaccc tctttaaatc ctttgcagcg cagttcaaga gccgcattac
      421 ctacttcctg gttgacgcag agggcatgtt tgacaccggc ggcacccatt ttctcaccac
      481 caccgatacc agcctgttct tcggtctgtc caatgtgaac cagcactccc aaactcttcg
      541 caacatcgga gccagagttg atggaaatca ccggaatacc agcagcaaca gccgctttaa
      601 tcgaaggacc aagagcattg gcatcgggga tcgagacaac cagcccactt gggtgggccg
      661 acaccgcagt atcaatgagg tgggccatgg ctaccacgtc gaaagtagcc ccggttggcg
      721 attcatagat ggccgtgaca cacatatcct tggccgcctg gtcaacacct ttcttgacca
      781 ccgaccagaa tgggtctgac gcctggccgt gcgtaatcac aaagaaatgg aggctcgatc
      841 gcgacgacgg acacgaactg gaactactac ttgtcggccc gctactgccg caagcagcaa
      901 ggaccagaat taagatcatt gccggtgcga agctgaataa tctacgcatt gtttcctcct
      961 ttgggatcat agttgttttc cctgcgaaca gggtccgttt cacttgatgc gcgcgtcatg
     1021 gtgcgcgaat cgacacgccg ttaggtcttc gaggactcct tcccgataat gacctccttt
     1081 cgtggaacaa agcgttcctg aacagcatct atggcctggc atcggcagat agaggaccct
     1141 gttgctctag ataatagcgc tcctgcgctc gcatcgcttc ccactcttgg cgggccgctt
     1201 gaaccgaggg cagagcgctg acttcagcaa caggcacatc ccaccagctt tcatatccgg
     1261 gtacgccttc atagcggtca ttctgaatgc agataacggt cgtttgaccg gtcgtgcgcg
     1321 cttgcttaag cgccttcacg aaatcctcat aggtattgca ttcgataacc agcgcaccca
     1381 ggctgcgcgc gttggccgcc aggtcaattg aaagcgtctg cgcgtcagat cccattgcat
     1441 cgcctggcaa tatcccattg cgcggataga cgtagcgcgt cccaaaacca tcctggccaa
     1501 gcgagcggct caggctgccg atgctcttaa acccgtgatt atcaaacagg actatgatga
     1561 gcttatagtt ctcctggatg gaggtgacga cctcgctgtt catcatgaga taggaaccat
     1621 cgccgaccat cacgtacacc tcccgctcag gcgcggccat cttcgcaccc agcccaccgg
     1681 cgatctcgta acccatgcag ctatagccat attccatgtg gtagtttttc gggtggcgcg
     1741 cccgccagag cttgtgcagg tcgcccggca ggctgcccgc cgcgcagacc atgacggcat
     1801 ccggcgcgct gagctcgttg accgcgccga tcagctcgcc ctggctgggc agcggcgtgt
     1861 ggcggatggc gtagatgcgt tcgacttcgg catcccatgc cgcttgcagc tgcgccgcct
     1921 gcgcccggta gctctcctcc acgtgatagt cctccatcag ggccgacaat tcctccagcg
     1981 tggcctgcgc atctccaacc agtgacagcg cgtgatgctt ggcggcatcg aacgaagcca
     2041 cgttgatatt tatgaagcgc acattggggt gctggaaggc cgtcttggag gcggtcgtaa
     2101 agtcgctgta gcgcgtgcca atgccgatca ccaggtcggc ctcgcgcgca atgcgattgg
     2161 cggcaaacgt gccggtcgcc ccgatcgcgc cgaggttgag cgggtggtca tagcgcaggc
     2221 tgcccttacc ggccatcgtc tcccccaccg ggatgccggt cagctccacc accagccgca
     2281 gcaggtcggt cgcgccggag tagatcacac cgcccccggc cacgatgagc ggacgctggc
     2341 tggcgcgaat ccaggcggcg gccagttcga gcgcgccgcg atcggggcgg ttgcgcgcca
     2401 cgcgccacaa gcgccgttgg aaaaaggctt ccgggtagtc atacgccatg gcctggatat
     2461 cctgcggcag ggccagcgtg accgcgccgg tctccgccgg gctggtcagc acgcgcatgg
     2521 cttcgggcag cgcagtcaaa agttgctccg ggcgcatgat gcggtcccag tagcggctga
     2581 ccggtttgaa acaatcgttg accgaaatat cctggctgtg ctcggattcg agctgttgca
     2641 agaccggcgc gacattgcgg cgcgcgaaga tatctcctgg caagagcagc acgggcagac
     2701 ggttgatggt ggcagtcgcc gcgcccgtga ccatattcgt cgcaccgggg ccgatggacg
     2761 aggtgcaggc aaaggcgcgc aaacggttgt ggactttggc gaaggccgtc gcggtatgca
     2821 ccatggcctg ctcgttgcgc gcctggtagt agcggaactc cggcatctgt tgcagcgcct
     2881 ggccgatgcc ggcaagattg ccatgcccga agatgccgaa acacccggcg aagaatgcct
     2941 gctcgacgct atcgcgctcc acaaactggt ttttgaggaa ggcgatcagg gcctgcgcca
     3001 tcgtcaaacg tcgcgtggtt tgatgattcc taatgttcat cttcgcgtcc tcttttgctg
     3061 ctaaaatccg ccgtgatctt gaacaaaggc catgacttca tcataggtcg gcatgaaatt
     3121 cgcacagccg tgctttgtca ccaggatggc gccgcaggca ttgcccaggc gcgcggatcg
     3181 ataccagtcc cagcctttga tgaagccata caggaagccg gaggcgaagg cgtcgcccgc
     3241 gcccaggatg ttctgcacct ctaccgggaa gcctggcaca tcgatgcgac gggcttcgcc
     3301 atccgtgaga tgcactctgg aaccttcttc gccgcgcttc tgcaccagca cgcgcggccc
     3361 caattccagc aagcgctgga tggcggcatc ggtatcgccc agtacgcgcg cgtcagaaat
     3421 ctgggaatgc gcgatgctga cctggcgcac atcggtgagc atggccgctt taatctcgtc
     3481 ttcggtgccg atcacgatat cgaccaggcg cagcgccgag cgcagcgtca caccaaaggc
     3541 gcgctggtca acccactggt cggggcgata gtcgatatcc agcgcaaccg gtacgtccgc
     3601 ctggcgcgcg gtctcagccg cgaacagcgt ggcgctgcgg ctcggctcct tgctcaggcc
     3661 ggtgccggtg atgagcagcg cgcggctatc gacaagcgga gcagccagcg catcatcgat
     3721 agtcagggca atatccgcgc aattgtcgcg gtagtacgtt aagggaaagc ggtccggcgg
     3781 ctcgatgccc agcaccaccg cgctcgaacg tcgccctggc ttgcgcggga tgtagcgcgt
     3841 ctccacgccc tcttcgcgca ggaagcgcag gatgaagttg ccgacctggt cctccccgac
     3901 cgccgtcagt agcaccggac gcaggccgag ccgccgcatt cccacgctga tattcgtcgg
     3961 gcagccgccg acataggccg cgaaactcgt gatctcctcg aagggcgcgc tgatgtcgtt
     4021 ggagtagagg tcaatcgacg accgccccat tgcgacgata tcgtaggtct tgtgagtcgt
     4081 atccatagct gactccaggg ttaagacggg ttttctatcc acgctgttcg atgtcgtaga
     4141 tgggtacgcg gggatcgcgc gagcgatatt gctgcttgac ccaggcgtaa cgcgggtctt
     4201 cgctgttcgc aagcgattgt gcgctccccg ccagcacatt caggtagtag gttgtgtatc
     4261 cgggagcgcc ggagacggga tgatagccct ccggcaccag cacgacatcg ttgtcgcgcg
     4321 ccagcagcgc cgcgtcgata ggatttccgg cgcgatgcag cggcgattcg ggcaccgtat
     4381 agatgcgttg aaaggcaaat ccctccggtt tgtcaaattt gtaaaaatag atttcttcca
     4441 gatcagcttc aataagcttg tgctgttgat cggttttgtg tacatcatgt ttatgtgggg
     4501 gatagctcga ccagttgccg ccgggtgtat acacctcgac gaccaccagg cggtcacagg
     4561 ggaaaccggg tggaatgatg ctgttgatct gccgggtcgc attatcgccg ccgcgtatct
     4621 cgatctcgat atcgcggggc gtgacaaagc gcggctcgcg atcctggtcg gtcggcgcaa
     4681 gcgctacagc gaattcgcag tcactctccg cgcgaacagt gagattcgtc cggcgaggaa
     4741 aatagagcgc ggacggcaga ccatcgaata catgcttgcg ctgaccaatg cgcgaccatt
     4801 ggtccttctc agactcgacg gtcaattgac cgcccaggac gacgatggcc agttcataac
     4861 cgccggtagc gaatgtccag gaacgaccag ccgccaggcg gcgcacttga aacgagacat
     4921 attcccaccc ggccaggtct ggggttactt cgatgacgac atcgggatcg ctcgaattgc
     4981 ccggatgaac gacgaggttg tccgcgtgat actgacgcat aaatatactc cccgtagagc
     5041 tagacaaaac tgtgaaagtg ggtatacgcc tccgtaggtg ccgattgatc gcgcacatcg
     5101 ccgattgatc ggccacaatg ggcgtgaacg gcaggcatcc aggccgataa tgatttttaa
     5161 cgatcaagtc cggcaatgtc atccggtcat gctcttccac cagggcgcaa tgaattgcgc
     5221 ccctacaatg ccgcatcatc caattcgctc agcgccaccg ggcgtcgcgt gtgataggac
     5281 tgcgtggcgg cgatgccgac tttacaggct gcgtatgcct cggctccccc gaccgaggga
     5341 gtttcgcccg cgcgcagaca atcgacaaag tgttgcagtt gcgcgcggta ggcgtcaccg
     5401 aagcgttcca tcaggtaggg catcacgtcg tgctttgcgc cggcgcgggt cagcagcacc
     5461 accggcgtgt attgatgcgc gccaatcatc accgcgcctt ttgagccaag cacttcggtg
     5521 cgaatatcgt agccatagaa ggcgttgcgg ctcgcttcga tgttgccaag cgccccgctc
     5581 gcaaagcgca tgtttaccag ggcattatcg atatcgccga ccgaggccag ttcttcgcac
     5641 accagggtcg tgccttccgc cgatacacgc tcgaccccgc tgccaaccag ccagtgcgcc
     5701 aggtcgatat cgtgaatggc catgtcgata atcaggccgc cgctttgcgc ggggtctaga
     5761 tagttgcgcg gcgggcagaa cggatcgcga ctgagcgatt tgaacgtgac agggtagccg
     5821 atctgcccat cgtcgatcat ggctttcgcg cgctggtatg ccaggtcgaa gcgccgcata
     5881 aagccgacct gcagcggaac cttcgccgat tcaaccgcat ggatgatcga gcgcgtctcc
     5941 cgcagcgtca gcgcgagtgg tttttcgcag aagatggcct tgccttctct cgctgcggca
     6001 attgccagat cgtgatgcgc gctggtcggc gtggcaatca ccaccgcctg cacgtctgga
     6061 tgcgcgatca actcatacgc atcggcatag gagcgcggca cattcaactc gctttccact
     6121 tgagcgcggg tcgtctcgcc cacttcggca acggcgtaga gctgtgcgcc cgtaacctgg
     6181 gttgcgaggg cttgcgcgta taagcgcccc atgcgaccca gaccgatcac cccaatcttg
     6241 actcgagcag gcatatcagg cgaactcctt cctggctcct gccacgctca caggcaaggc
     6301 cgtgctggcg cgcgtaatca actctggttg caacaggtga ttcttccctg gacggttatg
     6361 gagtaaatcg agcatgactt ctgtcgccag gcggcccaat tccagctttg gctggcgaac
     6421 ggtcgtcagc gccggagaga catagctcgc cagcttgatg tcgtcaaaac ccaccacgct
     6481 caactggcca ggcacgtcta tcccccgctc gcggcaggcc atcaacaaac cgacggcggc
     6541 catatcgtta tagcagaata tggcagtgac ccggttatcg agaaagtgct ggagagaagc
     6601 ctgtccaacg gcgacatctt cttcatgcga cgcctcggtc ccggatgata ccagaatgca
     6661 tttctcgcaa aacgggatgc cagccgcagc cagcgcaccc tgatatcctt gaaagcgctg
     6721 ccggtttgac ctcggcctgc tgctgatgcc gagatagccg atgtctcgat ggccaagttg
     6781 tagtagatgt tcgaccgcga ggcacgcgcc aaagcggtca tccacggaca cccagttcaa
     6841 tagttcgttc tgcgaatcgg cctggctatt gataatgacc gttggaactc tgatatggtc
     6901 gagacgttgt ttatacgcgc tggtaatacg agacgatgcg accaggatgc catcgacgcg
     6961 gcggcgatgg aaggtttcga tcaccaccat ctcctgctca ggatcattgt gggtcgcgct
     7021 cagcaagacg ctgaaattcg ccgcgcgggc tacttcctcg acgcccttga cgacatcatt
     7081 cagaaaggga tcggcaattg aggtgaccac caggccaatc gtgctggtgc tgcgcgtctg
     7141 caaactctgc gcaatggcat tgggtgtata gcccatctcc tgggccagcc gctggatgcg
     7201 ctcgcgagtt tcgaggctga tgagatgatt cccacgcagc gcgcgcgata ccgtcgtatg
     7261 cgaaacaccg gcgacacggg caatatccgc gatggatatt tcatgctgac gcaacggagt
     7321 agcctttcgt cgataaagcg cgcaacgtca ccagtttgcg cacacgtgtg gaaggacatg
     7381 taagaaaatt tagatttaac cggcctatgc acacgtgtgc attactagta tgcagtatgc
     7441 tgctgggatt tgtcaaggga aaacgctgcc atattttgcg ccaaagcccg ccgtcataaa
     7501 tttgtcttga cttgctccat aaaccccatt gtggtcgcgc tgcctccctg gtcgacgggc
     7561 aaagccacac ccccctggta ggtctggcga atggcgcgct caagacgccg ggcctcttcg
     7621 tgatagccga gatagttgag cagcagcgcg ccggtgagca gcatggcggt aggattgatg
     7681 atgcccttac cggccaggtc gggcgcggag ccatgcaccg gctcgaagta ggcgtaatct
     7741 tctccatagc aggcgctggg cgagaggccg aggccgccga tacagccgct ggcagcgtct
     7801 gccagaatat cgccatgctc gttgccgaga acgacgacat ccagcgtctc cggctcggca
     7861 accatgcggc gcgccaggtc gtcgaccaga tattcctggt agcccagacc gggataggcg
     7921 ctgaccgcct ctttgacgat ggcacggaaa cgcccatcgg tgcgcggcaa gatagaatac
     7981 ttggcgccca gcgtgacgcg cccaggatag ccgccagcct ggcgttgcag ggctaactca
     8041 cacgcggcca gagccacgcg ctgcatctgc tcatcggttg cgacgcgcac ggcgtaagcg
     8101 cctctgctct ccacgggggg cgactgccac cagtccgcgc tgctggggag cgcgctcagt
     8161 tccgcgatgt cgccctcgcg cggcgggtat atgccttcca ggttatcgcg cacgatcaca
     8221 tagtcgatgc gctctggatg cagcatggga ctgcgatacc ccggactcca gcgcacaggc
     8281 cgtacattga caaaggtttg cttgccccag cgcaaatacc acaagaccgg cctgctcggc
     8341 ccgccactcg cgccgaaaag cgcgcaatct gcgtcatcga tggcccggcg cgtcgcggcg
     8401 gggaatgcct cgctgtgttg ctggatggcc gcgcgcccgc tcaacgcctc gacgaagcga
     8461 ataccgggcg cgagcaggcg cagcagctcg acggtcgggc gcatcacctc cggcgctgcg
     8521 tcgtcaccgg cgatgacgca gacctgcggc ggccccgtcc gagaccgccg ggaagccagg
     8581 gaatagacgc tcatcgcagc gtcccctcct ggcgaatgta gttgacgatg ccgccagccg
     8641 ccgcaatgcg cgccacgaag ggatcgagcg gctcggcctg gaaggtctgt ccagtcgtca
     8701 ggttctgtat cgcgccgctt gccaaatcga
//
LOCUS       DASZAK010000265        15783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_219845, whole genome shotgun sequence.
ACCESSION   DASZAK010000265 DASZAK010000000
VERSION     DASZAK010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 15783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15783
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219845"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..769
                     /locus_tag="VGT44_04565"
     CDS             137..769
                     /locus_tag="VGT44_04565"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2580106.1"
                     /translation="MKDTTDHQRPKAYHHGDLRNALIETGLALLSEGGARELDLRKVA
                     RKAGVSHAAPYRHFADKQALIAAINEVGFNRLAEQIQSTLREAPEDALAQLQGVAIAY
                     VQFSDENPWLMREMFSGLTIEREAFPALYEASKNVYRLYIAVVKRGQESGAIREGDPA
                     ALAGVLWSVLHGVAMLIIENQIRPYAEGPEGIDNVTRFAIQMLYDGLGRI"
     gene            817..1179
                     /locus_tag="VGT44_04570"
     CDS             817..1179
                     /locus_tag="VGT44_04570"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2580107.1"
                     /translation="MIEKITLADKFNRFSDHWKPKIAGELNDSYIKLVKLQGEFVWHH
                     HENEDELFLVIKGKLVIKLRDQDVTLEEGEFVIIPKGVEHCPVAAEEAHVLLLEPKTT
                     LNTGNVANERTVADPERI"
     gene            1809..4286
                     /gene="lon"
                     /locus_tag="VGT44_04575"
     CDS             1809..4286
                     /gene="lon"
                     /locus_tag="VGT44_04575"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV2580108.1"
                     /translation="MVTKKASRAKQRTGEIIELQNTHELPLLPIRNTVLIPNVVTPLM
                     VGREASIKAIESAMGRDRRLFVVTQRNEEVEDPGPEDVYAIGVEGVIDRVLKLPDGTT
                     SVLVHGQRRMRRLEFTQRLPFMRVQAVALPEETTLTPAIEAQSRAVLALFEKCVKLSN
                     VIPDEAYITAMNIEQPGWLADFVVSTIESPIPVRQDILETLDVEERLRKTYLLLNKEL
                     HILELENQIHTQVQLEVDKSQREYFLREQLKAIQHELGEIDPSMRDGEELREQIMKAG
                     MPDEVLAKALKELERFNAMPSMAPDSGVLRSYLDWLASLPWQRVTEDRLDIKEAARIL
                     EENHYGLDKVKERILEYIAVRKLSNTMRSPILCFVGPPGVGKTSLGRSIAEALGRKFV
                     RLSLGGVHDEAEIRGHRRTYVGALPGRILQTMKTAGTLNPLFVLDEIDKIGNDYRGDP
                     SAALLEVLDLEQNHAFSDHYLEVPYDLSKVIFLTTANVLHPVPAALRDRLEVIELPGY
                     TEDEKLHIASRFLIPRQTQEHGLKPEKLAFEDAATRRIIREYTFESGVRNLEREIATV
                     MRKVARKVAEGRRAKSVITAERIPDFLGPQQHFFELAEERDEPGIATGLAWTGAGGDL
                     VRVEATLMEGRGNLTLTGQLGDMMKESAQAALSYARSRAAQLGLESRFYEKYDVHLHL
                     PSGAIPKDGPSAGITMATALISALTKRPARRDVAMTGEITLRGRVLPIGGIKEKTLAA
                     HRAGIKTLILPKRNMKDLDEIPPEVLSDLRFVPVERMDEVINVALHALPLTQEELDAP
                     EPLLRPIKPIRVRRAPVARPAPLPVTN"
     gene            complement(4283..4651)
                     /locus_tag="VGT44_04580"
     CDS             complement(4283..4651)
                     /locus_tag="VGT44_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580109.1"
                     /translation="MQGKRPKILVVDDDPDILDFLQDLLNLENYTVVITEQAEYLEQL
                     HDGGLPDLILIDILLSGKDGREIIRHLKSQERNRYVPVIMFSAHPSAETTALEAGADD
                     FLAKPFEMDELLAKIKKWTS"
     gene            complement(4680..6167)
                     /locus_tag="VGT44_04585"
     CDS             complement(4680..6167)
                     /locus_tag="VGT44_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV2580110.1"
                     /translation="MAITPQQEAYSLEVEIAERKKVDERLRTLAAIVESSDDAILSKD
                     LEGTITSWNRAAERIYGYTAEEMVGQPVTLLFPRGRQDEFTGIMARIRRGERVDHYET
                     QRVSKDGAVLTVSVTVSPVKNSAGVIIGASAIARDITEQKQLESKSRQLFDSSLIGVF
                     VSDFDGVFLDANNAFLKLIGYTREEVQAGVMRRDTITPLEYHILSQQEVKALRETGAS
                     ETYEKEYIHKSGKRIPVVVAVTRIEHTDTCIGFVLDISERKELDRRKDEFISMASHEL
                     KTPITSLKGFLSLLHRLLAEQGNEQGLHYLTRMDTQVNKLNRLISDLLDISKMQTGQI
                     VYREERFDMDELVQEIVEDIQGTTQTHQLMLEGRIQAEMFGDRDRIGQVLINLLNNAI
                     KYSPKADTVLIKIGKDRNMALLSVQDEGIGIAEENHSRIFERFYQVTDPVANTYPGLG
                     IGLYISCEIVKRHGGDLWVESQRGKGATFHLSLPVIQESKKENFV"
     gene            complement(6290..8617)
                     /locus_tag="VGT44_04590"
     CDS             complement(6290..8617)
                     /locus_tag="VGT44_04590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2580111.1"
                     /translation="MSLEGQQLGRYHLQQVLGMGGMGEVYLATDSVIHRQVAIKVIRD
                     EASAYPNPTMSQESARLFQREMKAIATLDHPHILPLYDYGEENVKGAKLSYLVMPYRP
                     EGTLATWLQQRPGGGLLPLAVVAFLVEQAADALQHAHDHQIIHQDVKPSNFLLRVKRE
                     DPDHPDLLLADFGVSKFSSATANVSQSIRGTPASMAPEQWEGHPVPATDQYALAVMAY
                     ELLTGRPPFQGGLSQLMYQHFHAMPPRPGSINPRLPAEIDDVLLHALAKRPEQRFANI
                     AAFGRAFQQAALSGAQTMVDDFQPVNPPTPFPTPHLTPLPTPHLTPIPPPPPAPGTQT
                     DIYAVLAISPAEARSGGSRTITLPGGRQMMVPLPAGAVDGQIISLQDRQGPGSAPMTL
                     NLRLSIQQTDSSGAISAPGSADQTMRGASSEWMPPAQPARQPTGEQRPVVPAAPITPV
                     VPIAPPPPGIALGSQNLSAASNIRQSGPAYAPKQAGGTGGSEPGERPRRRASTPKLRT
                     ALLVGLALLLVLGSIGIFAAVRNGQAATASANATAQAQATAFPKNATSTANANVQATQ
                     VAQYYGMLTATATAFQNLYSGSTSGNPNLNDPLGSNMLGYGWQEGTFNPGGACKFSGN
                     AYHVSITAANYYQPCIAGRTNFANFAFEVQMKIINGDCGGLIFRGNSANGQFYYLRIC
                     QDGAYALWDELDSYKSQSTELTSGNSSAVTNGLNQTNQITVVAKGNTITFYVNRQGVD
                     SVTSNTYSHGEIGLVADYLNSATEVAYSYAKVWVL"
     gene            complement(8827..10506)
                     /locus_tag="VGT44_04595"
     CDS             complement(8827..10506)
                     /locus_tag="VGT44_04595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2580112.1"
                     /translation="MQFLHKNRPDPEHLPWPLPVKLSVIVTLALGLILSWQLWLHNLF
                     NAYDFSPHGYCYLWLPGLVTLHVSSDALIGLAYVSITSTLLYFVIKTRHDVPFQWIFI
                     AFGLFIVACGATHFMEIVTLWYALYWLSGAVKLVTAVASVTTAIALPFMIPEIRAVIA
                     SAKLSEERQRQLEQAHRELQVLYQQSEDALHRSEQRYGDLIESLPLVVWTADAEGNVR
                     YHNRYWYEYTGVDREQAREGGDSAVTQVHPDDRERILDDWKTVLRAGEPYETEYRLKY
                     VSGDYRWQLARAVPIRDAQGHLTSWIGTDTDIEELKRVQKELEESEARKDAFLSMASH
                     ELKTPITSISGYTQVLKRLFVKEGRTQVVQHLSRMEYQIDRLVKLISDLLDISKIQAG
                     KLEFEEEEFEVCAFIRNIVEDVQQISPGHTLNLRCGMNATLVGDKDRLGQAIINLLTN
                     AIKFSPNAGSVDITVEEREGQAVFSVRDYGIGIPAAHQARIFERYYRVFDERDKKFPG
                     LGIGLSIVAEVVRRHGGELRVESDEGQGATFSFALPLRAATPRALALPNPS"
     gene            complement(10611..11510)
                     /locus_tag="VGT44_04600"
     CDS             complement(10611..11510)
                     /locus_tag="VGT44_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEV2580113.1"
                     /translation="MRLRRSELATPASSERMIEKAAAGSADLVFLDLEDAVAPNEKVA
                     SRAKVIHALKTLDWGKKTRAVRINDLETEFAYQDIITVVEEAGEHLDMLIIPKIKSAR
                     DVWWVDVLLTQIEKRLKRARRIGLEVLIEEVEAMVNVEEIARASSRLEAIVFGHIDYS
                     ASQGIDTSLAGNDLDTYPGDLWHYARNRITIAARAAGIDALDGPYIDFKNPVGYRREC
                     LRAKALGMVGKWAIHPGQIDIANEVFSPTPAEVERARKLDALYSEAQASGQGAVAFEG
                     KLIDVAVVRSARNVIQKADLIGM"
     gene            complement(11514..12035)
                     /locus_tag="VGT44_04605"
     CDS             complement(11514..12035)
                     /locus_tag="VGT44_04605"
                     /inference="COORDINATES: protein motif:HMM:NF039588.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEV2580114.1"
                     /translation="MQYGRCFEEFEIGAVYKHWPGRTITEYDDTLFSMLTMNHNPLHI
                     DAHYAETMQHGQRLVVGPLIFSIALGMSVEDVSGNAIANLEFENVRHLAPTFHGDTIY
                     AETTVLEKRLSSSKPDRGVVTVETIAYNQRGETILSFKRRVLVPTLAAHEAWQRAWDE
                     KMNKLREEHKQGG"
     gene            complement(12292..13440)
                     /locus_tag="VGT44_04610"
     CDS             complement(12292..13440)
                     /locus_tag="VGT44_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2580115.1"
                     /translation="MEDTFWQPIIDEVRRFVEREVLPVAHEMEQHDIYPAELVEKLKT
                     LGIFGATIPEAYGGLGMHFVAYTQIIEELSRGWMSLAGIINTHVLVAYMIATYGTEEQ
                     RRALLPVMAEGAQRGALCLSEANAGSDVQAITMTAVREGDDYVLNGSKLWVTNGQRAG
                     IFAVLARTDPGAKPPHRGMSLFLVEQGAPGLSAARTFEKLGYKGVETVELVFDAVRVP
                     ANRLLGGVEGEGFKHAMSGLEVGRINVAARAVGVAQAAFNDAMRYAQQRHTFGKPIAQ
                     HQAIQIKLADMATHIEAARLLLRQAARKKDGGERCDMEAGMAKLFATEMCQQVTLEAM
                     RIFGGFGYTKEFNVERYYRDAPFMLVGEGTSEIQRLVIARQLLERFKE"
     gene            complement(13442..14362)
                     /locus_tag="VGT44_04615"
     CDS             complement(13442..14362)
                     /locus_tag="VGT44_04615"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV2580116.1"
                     /translation="MDTLNLHQLQTFQVVAKHCSYVRAAEELHFSQPAVSAQIRLLEQ
                     NLGVKLFEQIGRKTHMTQAGEELYLYSKKIFSVIDEAMEVMEALRSPHYGRLSVGADT
                     TVGSYVIPGMLGKFRQSFPAVEISLEVLNRLSLVDALLSNRVDLAVMGAIPDDAPVEI
                     EPIAYNSLVLISSPHHRLAGQSDVSIKELGREHFLLREPGSGTRAALETALRESGVPL
                     QISMQVGNNSAIKQGVAAGLGIALISSVALEMELETHRLVILDVEGFPIMRQWRLVHL
                     KDKYLSPTAHAFKAFMLQNADKRLRRKPQV"
     gene            complement(14520..>15783)
                     /locus_tag="VGT44_04620"
     CDS             complement(14520..>15783)
                     /locus_tag="VGT44_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2580117.1"
                     /translation="FIITSVLLLVIVFLAAFIPTVIQLVSSGSSSSSSGQTSIVVVNE
                     AGSVAGLNEAQLTADINTALNGTNTGSSAPFVLSIQPQTSLSSLQSQVKDGQLNMLLV
                     LDRPTNQDLRFTYYTTNGSTNDPNLSTVQGLTQLLAFLDTAHRLGLTSQETQHLGAAP
                     DLVVVQTKPGQATQVASESAAGYVLATAGVILIFISIQVYAGIVATGVAEEKSSRMME
                     ILVNAATPFQLLAGKIAGIGAACLTQMGGLVVVGIGALLLQTPVQAALFGTNAGGFSQ
                     YLTGVSIPFYLLFLLYFLLAFFLYATLFAGLASLVKRQEEVQSVIMLPLLLLSSGYVL
                     FFFVVATPDATVTKVLSYIPFWTPIFMLLRLAAGTVAWWEIVVTVALMLLTILACTWF
                     AARLYRYGVLMYGQRPGLGQLLKLVRMN"
BASE COUNT         3373 a         4636 c         4402 g         3372 t
ORIGIN      
        1 ttctctgcca ttgcaactac cgcagtgttg ctgtatgatg acaacgttaa cattctacca
       61 cagaatgttt tcattgtcaa cattgtttct gttgtaaact ttctgcggaa agctatcatg
      121 taatgggaag catgagttga aagacacgac ggatcatcaa agaccgaaag cctatcatca
      181 cggcgacctg cgcaatgctt tgattgaaac cggtcttgca ttgctctcag aaggtggagc
      241 gagagaactt gatttacgta aagtggcgcg taaggcgggc gtgagtcatg ccgcaccgta
      301 tcgccacttc gcggacaaac aggcgctgat cgcggcgatc aacgaagttg ggttcaacag
      361 gctcgccgaa cagattcaat ccacccttcg tgaggcgcct gaagatgctc tcgcccaatt
      421 acaaggggtt gcaattgctt acgtccagtt ttccgacgaa aatccctggc tgatgcgcga
      481 aatgttcagc ggcctgacca tcgaacgcga ggcatttcca gctctgtacg aagcatcaaa
      541 aaatgtctac aggctctaca tcgctgtagt aaagcgaggg caggagagcg gcgcaattag
      601 agagggtgat cccgccgcgc tagccggtgt cttatggtct gttctgcacg gggtagccat
      661 gctcatcatc gaaaatcaaa tacgccccta tgccgagggg ccagagggta ttgacaacgt
      721 gacccgcttt gccattcaaa tgctctatga tggcctgggt cgtatataat aggacgatga
      781 tagagctgtt atgaaaacaa agaaagcaga caaccaatga tcgagaaaat caccctcgct
      841 gacaaattca accgcttttc ggaccactgg aagccaaaga tcgcgggcga actgaacgac
      901 tcgtacatca agctggtgaa actacaaggc gagttcgtct ggcaccacca cgagaacgaa
      961 gatgagttat tcctggtcat caaaggcaaa ctggtgataa aactgcgcga ccaggacgtt
     1021 acattagagg aaggcgagtt cgtcattatt ccaaagggcg tcgagcattg ccccgtcgcg
     1081 gcagaggaag cgcatgtgct gctgctcgag ccgaaaacga cgctgaacac gggcaacgtc
     1141 gccaatgagc gcacggtagc agacccggaa cgcatttaat gcacagtggc caacccggaa
     1201 cgcatctaat gtaggcgccg gtttaccgtg cgcaccgccg atttatcggc ctgcggagcg
     1261 cgtcggcaat ccactctggc cgatatgttt gaattaccat aaattggttc ttacgcataa
     1321 cctcgcccga aaagagttga gcccaaaatc ttgctcagtt ctcttcttga cagtaacgaa
     1381 caatagacga gaaagcaaga cggtctgcgt ctatctttat agaagacaag tgtcatctcg
     1441 aagcgtctct attgcttcag aagggcaatt tcatgaaaac catcttcaat atcaataaca
     1501 gaggaaaatc catcctctac attcttttcg cggttgcttc cctctcattt gcagctctcc
     1561 ttctcttccc aattttgctc catggggaca tttcccccct tgccctcatc cctttcatgg
     1621 gtatagtgtt ccttggtagc attcttattc agatgaggag tggccccccc tggtagacac
     1681 ttgtgtgcag tattcgtcaa ctaggacttt ttctgacatt ctgtgccagt tctgttctac
     1741 ccgcttaaga tgcgttataa ttcagacaca ttaataaaga tgataaaaag ccagttgaga
     1801 ggatgtgtat ggtcacgaaa aaggccagcc gcgcgaagca gcgaaccggc gaaataatag
     1861 agctgcagaa cacgcatgaa ctccccttgt tgcccatccg caataccgtc ttgattccca
     1921 atgtcgttac gccgcttatg gttgggcgcg aggcttcgat caaagccatc gaatcggcca
     1981 tgggcagaga ccgcaggttg ttcgttgtga cacagaggaa tgaagaggtc gaagaccctg
     2041 ggccggagga tgtctacgcc atcggcgtcg aaggggtgat tgatcgcgtt ttgaagctgc
     2101 ccgatggcac aaccagcgtg ctggtgcatg gccagcgccg gatgagacgg ctggaattta
     2161 cgcagcgcct gcccttcatg cgcgtgcagg cggtggcgct ccccgaagaa accacgctta
     2221 caccggcgat agaggcgcaa tcgcgggcgg tcctcgcgct cttcgagaaa tgcgtcaagc
     2281 tgagcaacgt cattccagac gaagcctaca tcaccgccat gaacatcgag cagccgggct
     2341 ggctggccga cttcgtcgtc tccaccatcg aatcacctat cccggtgcgc caggatatcc
     2401 tggaaacgct cgacgtagaa gagcggctgc gcaaaaccta cctgctgctc aacaaggaac
     2461 tccacatcct ggagctcgag aaccagatcc atacccaggt gcagttggag gtagacaaga
     2521 gccagcgcga gtatttcttg cgcgagcagc tcaaagccat ccagcacgaa ctgggcgaga
     2581 ttgacccgtc catgcgcgac ggcgaggaac tgcgcgaaca gatcatgaag gccgggatgc
     2641 cagacgaggt gctggcgaag gccctcaagg aactggagcg cttcaacgcc atgccatcga
     2701 tggcgcccga ctcaggcgtg ctgcgtagct acctcgactg gctcgccagc ctgccctggc
     2761 agcgcgtgac cgaggatcgg ctggatatca aagaggcggc gcgcatcctg gaagagaacc
     2821 attacgggct ggacaaggtg aaggagcgca tccttgagta tatcgcggta cgcaagctct
     2881 ccaatacgat gcgcagcccc atcctatgct tcgtcggccc gccgggcgtg ggcaaaacct
     2941 cgctgggccg ctctattgcc gaggcgctcg gtcgcaagtt cgtgcggctc tcgctcggcg
     3001 gggtgcatga cgaggccgag attcgcgggc atcgccgcac ctacgtcggc gcgttgccgg
     3061 ggcgcattct ccagaccatg aagactgccg gcacgctcaa cccgctcttc gtactggacg
     3121 aaatcgacaa aatcggcaac gattaccggg gcgacccatc agcagccctg ctcgaagtgc
     3181 tggatctcga acagaaccac gccttctccg accactacct ggaagtgccg tatgaccttt
     3241 caaaggtgat tttcctgacg acggccaacg tgctgcatcc cgttcccgcc gcgctgcgcg
     3301 accggctgga ggtcatcgag ctgcccggct ataccgagga cgagaagctg catatcgcca
     3361 gcaggttcct gatccccagg caaacgcagg aacatgggtt gaagccggag aagctcgcct
     3421 tcgaggatgc cgccacgcgc cgcatcatcc gcgagtatac cttcgagtcg ggtgtgcgca
     3481 acctggaacg cgagatcgcg acggtaatgc gcaaagtggc gcgcaaggtg gcggagggcc
     3541 gccgcgcgaa gagtgtgatt acggcggagc ggattcccga tttcctgggg ccgcagcagc
     3601 atttcttcga gctggccgag gagcgcgacg agccgggcat cgccaccggg ctggcctgga
     3661 ccggcgcggg cggcgacctc gtgcgcgtag aggcgacgct gatggagggg cgcggcaatt
     3721 tgacgctgac ggggcaactc ggcgacatga tgaaggaatc ggcgcaggcc gcgctctcct
     3781 atgcgcgctc gcgcgccgcg cagcttggcc tggagagccg cttctacgaa aagtacgatg
     3841 tccacctgca cctgccctcc ggcgcgatcc cgaaggacgg cccatccgcc ggtatcacga
     3901 tggcgaccgc cctcatctcc gcgctgacga aacgcccggc gcgccgcgac gtagccatga
     3961 cgggcgagat cacactgcgc gggcgcgtcc tgccgatcgg cggcatcaag gaaaagacgc
     4021 tggcggcgca tcgcgcgggc attaagacgt tgatcctgcc caaacgcaat atgaaagacc
     4081 tggacgaaat cccgccggag gtgctgagcg acctgcgctt tgtgccggtc gagcgcatgg
     4141 acgaggtaat caacgtggcg ctgcacgccc tgccgctcac acaggaggag ctggacgcgc
     4201 ccgaaccgct gctgaggcca atcaagccca ttagagtgcg tcgtgcgccc gtcgcgcgcc
     4261 ccgcgccgct acccgtaacg aattaggatg tccacttctt gatctttgct aacaactcat
     4321 ccatttcaaa cggcttcgcc aggaaatcat cagctccagc ctccagtgcg gttgtctcag
     4381 cgcttggatg agccgagaac atgattaccg gaacgtacct gttacgttcc tggcttttga
     4441 gatgcctgat tatttcacgt ccatctttgc cagagagtaa tatatcaatg aggatcagat
     4501 cgggtaaacc accatcgtgc agttgctcga ggtactcggc ctgttcggtg attactacag
     4561 tatagttttc cagattcagg agatcttgta gaaagtctaa aatatctgga tcgtcatcaa
     4621 ccacgagtat ttttgggcgt tttccctgca tatcctgcct cattttggct tgcctcagct
     4681 tagacaaagt tttctttctt gctttcctgg ataacaggca gactcaaatg aaatgtagcg
     4741 cctttgcctc gctgactctc cacccacaga tccccaccat gtcgcttgac aatttcacag
     4801 gagatataaa ggccaattcc cagtcctgga taggtgttcg caacggggtc ggtgacctga
     4861 tagaaccgct caaaaatcct ggaatgattc tcttcggcaa tgccaatccc ttcatcctga
     4921 acactcagga gcgccatatt ccggtctttt ccgatcttta tcagcacggt atctgctttg
     4981 ggagaatact tgatggcatt gttcaggagg ttaatcagca cttgcccgat acggtcgcga
     5041 tccccaaaca tctcagcctg aatgcggcct tcaagcatca actgatgcgt ctgtgtcgtg
     5101 ccctggatgt cttccacaat ctcttgcacc agctcatcca tgtcgaagcg ttcttctcga
     5161 tagacgatct gacctgtctg cattttggag atatcgagca ggtcgctgat gagcctgttc
     5221 aatttattca cctgcgtatc catgcgggta aggtaatgaa gcccttgctc atttccctgt
     5281 tcagccagga ggcgatgcaa gaggctgaga aagcccttga gactggtgat gggcgttttg
     5341 agttcgtggc tcgccatact gatgaactca tccttgcgcc tatccagttc cttgcgctcg
     5401 ctgatatcta gcacaaagcc aatgcacgta tcggtgtgtt caatgcgcgt tacggcaacc
     5461 accacgggta ttcttttgcc acttttatgt atgtactctt tctcatatgt ttccgaggcg
     5521 cctgtttctc tcaacgcttt cacttcttgc tgactcagga tgtggtactc aaggggcgtg
     5581 atggtatcac gacgcatgac gccagcctgc acctcttctc gtgtgtaccc aatcaattta
     5641 aggaacgcat tatttgcgtc gagaaaaacg ccgtcaaaat ccgaaacaaa cactcctatc
     5701 agactggagt caaacagctg cctcgatttg gattctaatt gcttctgctc ggtgatatcg
     5761 cgcgcgatag ccgacgcccc aataatcaca ccggcgctgt tcttgaccgg ggaaaccgtg
     5821 accgagaccg tcaggacggc gccatccttg ctcacacgct gtgtctcgta gtggtcgacg
     5881 cgctctccac ggcgaatacg cgccatgatc cccgtaaatt cgtcttggcg accccgtggg
     5941 aagagcagcg tgaccggttg gcccaccatt tcctcggccg tatagccata gatgcgctcg
     6001 gctgcccggt tccaactggt aatggtgcct tccaggtctt tgctgagaat agcatcatcg
     6061 gaagattcta caatagcggc gagagtgcgc aggcgttcat ccactttctt ccgttccgcg
     6121 atctcgactt cgagcgaata tgcctcctgt tgcggtgtga ttgccataag cttctttcta
     6181 acgctgttac gaaaaactgg tcacaagtga atgcagccaa aaaatctttt tctgctaact
     6241 gacacgaata acctgcctcg cgcgatacat cgtcaggtta aaggctattt tagagcaccc
     6301 acaccttcgc gtagctatac gccacctcgg tagcgctatt caggtaatca gccaccagac
     6361 cgatctcacc gtgactgtac gtattactcg tcacgctatc aaccccctga cgattgacat
     6421 agaaggtaat cgtattgcct tttgctacga cggtaatctg attggtctgg ttcagcccgt
     6481 tcgtaaccgc ggaggagttc ccggaagtca actcggtcga ttgcgatttg tagctatcca
     6541 gctcatccca gagcgcatag gccccgtcct ggcaaatcct cagatagtag aactgaccat
     6601 tggcgctatt gccgcggaat atcaagccgc cacagtcgcc attgataatc ttcatctgca
     6661 cctcaaaggc aaagttcgcg aagttcgtcc tgcccgctat acacggctgg tagtagttgg
     6721 cagccgtgat ggagacatga taggcattcc cggaaaactt acaggcgccg ccggggttaa
     6781 aggtgccttc ttgccagcca tagccgagca tatttgaccc aagcgggtca ttcagatttg
     6841 gattgccgga tgtcgagccg ctatacaggt tctggaaggc ggtcgcggta gctgtgagca
     6901 ttccataata ttgcgctacc tgtgtcgcct gtacattcgc atttgccgtg ctggtcgcgt
     6961 ttttggggaa agcggtcgcc tgcgcctgcg ccgtcgcatt cgcgctggct gtggcagcct
     7021 gcccgttgcg cacggctgcg aagatgccga tgctgcccaa caccagcagc agagccagcc
     7081 cgaccagcaa cgcggtcctg agtttcggcg tgctggcgcg tcgtcgcggg cgttccccag
     7141 gctcggagcc gcccgttcct ccagcctgtt tgggagcgta ggccgggccg ctttgccgga
     7201 tgttggaggc agcagataaa ttttgcgaac ccaacgcaat gccgggcggt ggaggagcta
     7261 tcggaacaac gggcgtaatc ggcgcggcgg gaacgaccgg gcgttgctca cccgtcggct
     7321 gccgcgccgg ttgcgccggt ggcatccatt cggaactcgc tccgcgcatg gtttgatccg
     7381 cgctgcccgg cgcgctgatc gcgccggagc tatctgtttg ctgaatcgat agtctgagat
     7441 tcaaggtcat gggagcgctg ccaggtccct ggcgatcttg caggctgatg atctgcccat
     7501 cgactgcccc ggctggcagc ggcaccatca tttgccgtcc gcctgggagg gtaatggtgc
     7561 ggctgccgcc cgaacgcgcc tcagccggac tgattgccag caccgcgtag atatcggtct
     7621 gtgttcctgg agccgggggc ggcggtggta tcggggtcag gtggggcgtc ggcaaagggg
     7681 tcaaatgcgg cgttgggaat ggcgttggtg gattgactgg ctggaaatca tcgaccattg
     7741 tctgcgctcc actgagcgcg gcctgttgaa aggcgcgccc aaaagcggcg atgttggcga
     7801 aacgctgctc aggccgcttt gccagcgcgt gtagcaagac atcatcgatt tcggcgggca
     7861 ggcgcgggtt gatcgaacca ggccgggggg gcatggcgtg aaaatgctgg tacatcaact
     7921 ggctcaaccc accctggaag ggagggcggc ccgtcaacaa ctcgtaggcc atcaccgcca
     7981 gggcatactg gtcggtcgca ggcaccggat gaccctccca ctgctcgggc gccatcgagg
     8041 cgggcgtccc gcggatcgac tggctgacgt tcgcggtcgc gctactgaat ttggagacgc
     8101 caaaatcggc cagcagcagg tcgggatgat cggggtcttc cctcttgacc cgcagcaaga
     8161 agttcgacgg tttgacatcc tggtggatga tctggtggtc atgcgcgtgc tggagcgcgt
     8221 cggcagcctg ttctaccagg aaggccacga ccgcgagcgg gagcagcccg ccgcctgggc
     8281 gctgctggag ccaggttgcc agcgtcccct ccgggcgata gggcatcacc aggtacgaca
     8341 gtttcgcacc cttgacgttc tcctcgccat aatcatagag cggcaagatg tgcggatgat
     8401 cgagcgtggc aatcgccttc atttcgcgtt gaaagagacg tgcgctctcc tgcgacatgg
     8461 tagggtttgg ataggcagac gcttcatcac ggatgacttt gatcgcgacc tggcggtgaa
     8521 tgacgctatc ggtggcgaga tagacctcgc ccataccacc catgccgagt acctgctgga
     8581 gatgatagcg gccaagttgt tgtccttcga gcgacatcgt accctcccct gatgcgtatg
     8641 cacctcaaca aaattctcat tactttcatg attagttggg attgtttcgc tcatagtata
     8701 ccgtttcaat gcgagatgca caatactcga ctacgctttt tccgctctca ccacaaatga
     8761 cacaatccaa gccagaaagg ttgcgttacc cgatcagttg cagcctggcg aaataagatg
     8821 gggatatcag gaggggttcg gtaacgccag cgcgcgtggc gtcgcggctc tgagcggaag
     8881 cgcgaacgag aaggtcgccc cctgaccctc atcgctttcg acgcgcagct ccccgccgtg
     8941 cctgcgcaca acctctgcaa cgatggagag gccgatgccc aggccgggga acttcttatc
     9001 gcgctcgtca aagacgcggt agtagcgttc aaagatcctc gcctggtgcg cagctgggat
     9061 gccgatgccg taatcgcgca cactgaagac cgcctgccct tctcgttcct cgacggtgat
     9121 atccacgctt cctgcgttcg gagagaattt aattgcattc gtcagcaggt tgatgatcgc
     9181 ctggcccagt cgatctttgt cgccaaccag cgtcgcgttc attccacacc gcaggttgag
     9241 cgtatggcca ggactgatct gctgtacgtc ctccacgata ttgcgtatga aggcgcagac
     9301 ttcgaactcc tcttcctcaa attccagctt ccccgcctgg attttggaaa tatccagcaa
     9361 atcggagatg agtttgacga gtcggtcaat ctggtattcc atgcgggaaa gatgctgcac
     9421 aacctgcgtt cgtccctctt tcacaaagag cctcttcaag acctgagtat acccactgat
     9481 cgaggtaatc ggcgttttca actcgtggct ggccatgctc aaaaaggcat ctttgcgcgc
     9541 ttcgctctct tccaattctt tctgaacacg tttcaactct tcaatatcgg tatccgttcc
     9601 aatccaggag gtcagatgtc cctgcgcgtc tcttatgggt acagcccgcg caagttgcca
     9661 gcgatagtct ccgctgacgt atttgagccg gtactcggtt tcatacggtt cacccgccct
     9721 gagaacagtc ttccagtcat ccaggatgcg ttctcggtca tcaggatgca cctgcgtcac
     9781 agccgaatct cctccctctc tcgcctgttc acggtctaca cccgtgtact cgtaccaata
     9841 ccggttatga tatcgtacat tcccttcagc atcagctgtc cagacgacca ggggcagcga
     9901 ttcgatcaaa tccccataac gctgttcgct gcggtggagc gcgtcttccg actgctggta
     9961 gaggacctgt agttctcgat gagcctgctc taactggcgc tggcgctcct cggaaagctt
    10021 cgcgctggca atcacggcgc gaatttctgg aatcatgaat ggcaaggcaa tcgcggtcgt
    10081 gaccgaggcg acagccgtca ccagcttgac cgcgccggac agccagtaga gcgcgtacca
    10141 gagcgttaca atttccatga agtgagttgc gccacaggcg actatgaaca ggccaaaagc
    10201 aatgaatatc cactggaatg ggacatcgtg acgagtctta atcacaaaat agagcagggt
    10261 cgaagtgata gagacatagg ccagcccgat caacgcatct gaactgacgt gcagcgttac
    10321 cagccccggc agccagagat agcaatagcc atgcggcgag aagtcgtatg cattgaatag
    10381 attatgcagc cacaactgcc aggagagtat gaggccgagg gccagtgtga caatgacgct
    10441 cagtttgacc ggcagaggcc agggaaggtg ttcagggtca ggtctgttct tgtgtagaaa
    10501 ttgcacttcg atcccctcca aacgctaata cccaataatg acctccttag tataacagaa
    10561 cattccatca gaaatcgata tggatagcca atttgggcac gcgacgggcg ttacatcccg
    10621 atcaaatcag ccttctggat gacattgcgc gcgctgcgca ccaccgccac gtcgatcagc
    10681 ttgccctcga acgccaccgc gccctggccg cttgcctgcg cctcgctgta gagggcatcc
    10741 agtttgcgcg ctcgctcgac ttccgccggg gtaggggaaa agacctcgtt cgcgatgtcg
    10801 atttgaccgg ggtgaatggc ccatttgccg accatgccta acgctttcgc gcgcagacat
    10861 tcccggcgat accctaccgg gtttttgaag tcgatataag gaccatcgag cgcatcgatt
    10921 ccggcggcgc gcgcagcgat tgttatgcga ttgcgtgcgt agtgccacag atcgccgggg
    10981 taggtgtcca ggtcgttgcc cgccaggctt gtgtcgatac cctgcgaggc gctatagtcg
    11041 atatgcccga agacgatggc ttcaagtcgt gagctggcgc gtgcaatctc ctccacattg
    11101 accatcgcct cgacctcctc gatcagcacc tccagcccga tacgcctggc tcgcttcagg
    11161 cgcttctcga tctgcgtgag cagcacatcg acccaccaca catctcgcgc cgatttgatc
    11221 ttggggataa tcagcatatc gagatgctcg cccgcctctt caacgaccgt gatgatatcc
    11281 tggtacgcaa actcggtttc caggtcgttg atgcgcacgg cgcgcgtctt cttgccccag
    11341 tccagcgttt tgagcgcgtg aattaccttc gccctcgatg ctactttctc gttgggcgca
    11401 accgcgtcct caaggtcaag aaacaccagg tcagcgctgc cagcggcggc cttctcgatc
    11461 atgcgctcgc tgctggccgg ggttgcgagt tcagagcgtc tcagtcgcat tgatcaacct
    11521 ccctgtttat gctcttcgcg caatttattc atcttctcgt cccacgcgcg ctgccaggct
    11581 tcgtgggcgg ccagcgtggg aactagcacg cgccgcttga aggagaggat cgtctcaccg
    11641 cgctggttat aggcgatggt ttccacggtg acgaccccgc gatccggctt gctgctcgac
    11701 aggcgcttct ccaacaccgt cgtttcggca tagattgtat cgccgtgaaa tgtgggcgcg
    11761 agatggcgca cgttctcaaa ttccaggttg gcgatggcgt tgccgctcac atcctccacg
    11821 ctcatcccca gcgcgatgct gaaaataagc ggtccgacca ccagccgctg cccatgctgc
    11881 atcgtctcag cgtaatgcgc gtcgatgtgc agcgggttat ggttcatggt gagcatggag
    11941 aacagcgtgt cgtcgtattc ggtaatggtg cgccctggcc agtgcttgta gaccgcgccg
    12001 atctcaaact cctcgaaaca tctgccgtat tgcatcgtag ttcctctctt cttgtatcaa
    12061 gtcgcgccca ttagaccctc aaggttcaag cgaaatgtag gggcgcaatg atggacagta
    12121 aataattaat gagatgtacc cgtaggtgcc gatttatcgc gcacaccgcc gattgatcgg
    12181 ccaccatggg cgtgttcggc gggcaaccag gccgatcaat cggcggtgtg cgcgataaat
    12241 cggcacctac gggtacatct cattaattat ttactgtcca tcattgcgcc cctactcctt
    12301 aaatcgttcc aaaagctgcc gggcgatgac gaggcgctgg atttcgctgg tgccttcacc
    12361 gacgagcatg aagggcgcgt cgcggtagta gcgttcgacg ttgaactctt tagtatagcc
    12421 gaagccgccg aagatgcgca tggcctcaag cgtgacctgc tggcacatct cggtggcgaa
    12481 aagcttggcc atgcccgcct ccatatcgca gcgctcgccg ccgtccttct tgcgcgcggc
    12541 ctggcgcagc agcaggcgcg ccgcctcgat gtgagtcgcc atgtccgcca gcttgatctg
    12601 gatagcctgg tgctgcgcga tgggcttgcc gaaggtatgg cgctgctgcg cgtaacgcat
    12661 ggcgtcgttg aaggcagcct gcgccacgcc gaccgcccgc gcggccacat tgatgcgccc
    12721 gacctccagc ccgctcatgg cgtgcttaaa gccctcgcct tccacaccgc ccagcaagcg
    12781 gtttgccggg acgcgcacag catcgaaaac cagctccacc gtctcaacac ccttgtagcc
    12841 cagcttctcg aaggtgcgcg ccgcgctcag gccgggcgcg ccctgctcga caaggaaaag
    12901 gctcatgccg cgatggggtg gctttgcgcc tggatcggtg cgcgccagca ccgcgaagat
    12961 tccggcgcgc tggccgttcg tgacccacaa ctttgagccg ttgaggacat agtcgtcacc
    13021 ctcgcgcacc gctgtcatgg tgatggcctg cacatcgctg cccgcgttag cctccgacag
    13081 gcacagcgcc ccgcgttgcg cgccctccgc catgacaggc agcagggcgc ggcgctgttc
    13141 ttccgtgcca tacgtggcga tcatataggc gaccagcacg tgcgtgttga tgataccggc
    13201 caggctcatc cagccgcgcg acagctcctc gatgatctgc gtatacgcca cgaaatgcat
    13261 tcccaggccg ccgtaggctt cggggatggt cgcgccgaaa ataccgagcg ttttcagctt
    13321 ctcgaccagt tccgccgggt agatgtcgtg ctgctccatc tcatgcgcga cgggcagcac
    13381 ctcgcgctcc acgaagcggc gcacctcatc gatgatgggc tgccagaagg tatcttccat
    13441 gctacacctg cggtttcctg cgcaagcgct tatcggcatt ttgcaacata aacgccttaa
    13501 atgcatgagc ggtgggcgac aggtacttgt ccttcaggtg aacgagtcgc cactggcgca
    13561 tgatggggaa gccctcgaca tccaggatca caaggcggtg ggtctccaac tccatttcaa
    13621 gcgccacgct cgaaatcagc gcgatgccca ggccagccgc gacgccctgt ttgatggcgc
    13681 tgttattccc cacctgcata ctgatttgca gcggcacacc cgactctctc agggcggttt
    13741 ccagcgcggc gcgcgtgcca gagcctggtt cgcgcagcaa gaaatgctcg cggcccaatt
    13801 ctttgatgga gacatcgctc tgtccggcca ggcgatgatg cggcgaggag atcaacacga
    13861 gggaattata ggcgatgggt tcgatctcga ccggcgcgtc gtcaggtatc gcgcccatca
    13921 cggccaggtc gacgcgattg ctgagcagcg catcgaccag cgacaggcgg ttgagcacct
    13981 ccagcgatat ctcgacggcg ggaaagctct gccggaattt tcccagcatt ccaggaatga
    14041 catacgaacc caccgtcgta tcagctccta cgctcaagcg gccatagtgc gggctgcgca
    14101 gcgcctccat gacctccatc gcctcgtcga tcaccgaaaa gatcttctta ctgtagaggt
    14161 acagttcctc acctgcctgc gtcatatgcg tcttgcgccc gatctgctcg aacagcttga
    14221 cgcccaggtt ctgctcgagc agccgaatct gcgccgatac cgccggctgg ctgaaatgca
    14281 actcttccgc cgcccggaca taactacaat gtttggcaac cacctgaaag gtttgcagtt
    14341 gatgcaggtt gagcgtatcc ataggccggt aagcctcccg cggtgagata taactgaaat
    14401 attatatcat atgctttttg cgacctctcg ggggtatagt ggcaagggaa ccgaaacccc
    14461 tataggagcg cggtaaaccg gctcccacag gggttcgatg ctcgtttgca cacatactgt
    14521 tagttcatcc gtaccagttt cagcagttga cccaggccag gccgttgccc gtacatcagg
    14581 acgccgtagc gatacaggcg cgcggcgaac caggtgcagg ccagaatggt cagcagcatc
    14641 aaggcgaccg ttacgacgat ctcccaccag gccaccgtac ccgcagcaag tcgcagcagc
    14701 atgaatatcg gggtccagaa cggaatgtac gacagcacct tcgtcacggt cgcatctgga
    14761 gtagcgacca cgaaaaagaa cagcacatag ccactgctca gcagcagcag gggaagcatg
    14821 atcacgcttt gcacctcctc ctgccgtttg accagtgagg ccagtccggc aaacagggtg
    14881 gcatacagga agaacgccag cagaaagtag agcaggaaca gcaggtagaa cgggatggac
    14941 acgccggtga gatactggct gaagcctccc gcatttgtac cgaacagcgc ggcctgcaca
    15001 ggggtttgca gcagcagcgc cccaatgccg accaccacca ggccacccat ctgcgtcagg
    15061 cacgccgcgc cgatgcccgc gatcttgccg gccagcagtt ggaagggggt cgcggcattc
    15121 accagaatct ccatcatacg gctgcttttc tcttccgcca ctcccgtcgc cacgatcccg
    15181 gcatagacct ggattgagat gaagatcagg atcaccccgg cagtagcgag cacatagccg
    15241 gcagcgcttt cgctcgccac ctgagtcgcc tggccaggct ttgtctgcac gacgaccagg
    15301 tcaggtgcag cgcccaggtg ctgggtctct tgcgaggtga ggcccaggcg atgagcggta
    15361 tccagaaagg cgagtagctg ggtcagaccc tgaaccgtgg acaggttcgg atcgttggtc
    15421 gagccgttcg tcgtgtaata ggtaaagcgc aaatcctggt tcgtcggacg atcaagcacc
    15481 agcagcatat tcagctggcc atccttgacc tggctttgca ggctgctcag agaagtctgt
    15541 ggctggatgc tcaacacaaa gggagcggag cttccagtgt ttgttccatt caatgcagta
    15601 ttgatgtcag cggtcaactg cgcctcgttc agaccggcga ccgagccagc ctcgttcacc
    15661 accacgatac tcgtttgccc actggaactg gaactggaac cggagctcac aagctggatc
    15721 acagtcggga taaaagcagc cagaaacacg atcaccagaa ggagcacgct ggtgatgatg
    15781 aaa
//
LOCUS       DASZAK010000266         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_610891, whole genome shotgun sequence.
ACCESSION   DASZAK010000266 DASZAK010000000
VERSION     DASZAK010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610891"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="VGT44_04625"
     CDS             complement(<1..966)
                     /locus_tag="VGT44_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV2580118.1"
                     /translation="MMSGPSSPMPEQVVTTHVVGIDIGMESCTMACLTMDKRPVIKAT
                     PFANTLQGVDWLFERLEGLGVARPQILVGLEATSRYGENLLQALLKRGYQVCLLHPAQ
                     THAFTHQRGLRAKTDQLDAVTIGRVLLSGEARFGYVPSEQVATYRELMRLQKQLSDDV
                     VRYKNEIHALLVVLFPEFTQVFADPTRPTALAVLKRYPSAQAIAQTEVETLGKFLREL
                     APRHYGRPTAQELVGLAKVSISSGVAVAARSSSLHILCDQLDHTQKNLEQLQREIDQL
                     LDQDPKATGMLGMPEFGPTTVAVLRAELGDLARFTRIDQVVAYVGL"
     gene            complement(1391..>2003)
                     /locus_tag="VGT44_04630"
     CDS             complement(1391..>2003)
                     /locus_tag="VGT44_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2580119.1"
                     /translation="VQLTGAGLRERIELDRRADAFARSLLEPLNDSQRVKLAAAMAQV
                     ERLLIASMVQITVAYPTSQDARWCIEQYFTELGQRFETGFDPTLGISAHPHELVPPAG
                     LLLVACLRGEPVGCGALKFHENTVGELKRMWVAPRARGLGLGRRLLEALEHKAREAGM
                     TVIHLETNRTLIEAIELYRHSGYQEVAAFNDEPYAHHWFEKRL"
BASE COUNT          399 a          629 c          567 g          408 t
ORIGIN      
        1 gagcccaacg taggcgacca cctggtcgat gcgcgtgaag cgcgcgagat ctcccaattc
       61 tgcccgcagc accgccaccg tcgtcggtcc aaattccggc atccccagca tgccggtggc
      121 cttgggatcc tggtccagca actggtcgat ctcgcgctgc aactgctcca ggttcttctg
      181 ggtatggtcc agctgatcgc agagaatatg caggctcgat gagcgagcag cgacggccac
      241 cccactgctg attgacacct tggccaaccc taccagctct tgcgcggtgg gccgcccgta
      301 atggcgcggc gccagctcac gcaagaattt gccgagggtc tccacctcgg tctgcgcgat
      361 ggcctgagcg ctgggatagc gcttgaggac cgccagggcg gtgggccgcg tgggatcggc
      421 aaagacctgc gtgaattcgg ggaacagcac caccagcagc gcatggatct catttttgta
      481 gcgcaccacg tcatcactga gctgcttttg taagcgcatc aactcgcggt aggtcgctac
      541 ctgctcgctc ggcacgtagc caaagcgcgc ttctccgctg agcagcactc gcccaatggt
      601 gacggcatcc agctgatccg ttttcgctcg caacccgcgt tggtgggtaa aggcatgcgt
      661 ctgggccgga tgtaacagac acacctggta gccacgcttg agcagagcct gcaacagatt
      721 ctctccgtag cgcgaggtag cttccaatcc caccaggatc tgtgggcgag cgacccccag
      781 gccctcaagt cgctcaaaga gccagtcgac cccttgcagc gtattggcaa agggggtcgc
      841 tttgatcacc ggtcgtttgt ccatcgtcaa gcaggccatc gtgcaggact ccatgccgat
      901 atcgatcccc accacatggg tcgtcacgac ctgctcgggc atcggggagg aaggtccaga
      961 catcatcagt gctctttctc atcgccgccg tcctcctgct cacacaggaa aacctacagg
     1021 gacgtgcctc aacggggaag accgcccagt caggacgagc gagaactcat gtcggatcgg
     1081 cagtcttccc agcgcgtgcc aggtgctgca acaagcccca ttgtcgcttg cagtcctgat
     1141 ggacggggag cagtctactc acggcggtcg ctatccgttt ccaaacgcgc cgcatgttac
     1201 cacaaaggct ctcccgttgt ttccaccagt atagcagatg ccatctttct cagatggtct
     1261 ctcctatcta ccaattagta tatgagttac ccagggcgac ggccagcatc gcccctaccc
     1321 tctacttatc agtgtgagag ccaatttacc gagggtaggg gcgatgctgg ccatcgccct
     1381 gaacactggc tcaaaggcgc ttttcaaacc agtgatgggc atacggttcg tcgttgaagg
     1441 ctgctacctc ctggtatcca gaatgtcggt acaactcgat ggcttcgatg agggtgcggt
     1501 tcgtttccag gtgaatgacc gtcattcctg cttctctggc cttgtgttcc aaagcctcta
     1561 gcagtcgccg tcccagccca aggccacgcg cgcgtggggc gacccacatg cgtttgagtt
     1621 ccccgacggt attctcgtga aactttaaag cgccacatcc taccggctcc cctcgcagac
     1681 aggccacgag caacagaccg gctgggggta ccagttcgtg cggatgtgcc gaaatgccca
     1741 gggtaggatc gaaacccgtc tcaaagcgct gacccagctc agtgaaatat tgctcaatgc
     1801 accacctggc atcctgactg gttggatagg cgacagtgat ctggaccatg gatgcaatga
     1861 gtaaccgctc gacctgtgcc atggctgctg ccagtttcac gcgctggctg tcgttgagtg
     1921 gctccagcag agaacgagcg aaggcatcgg ctcggcgatc cagctctatt cgttcgcgaa
     1981 ggcccgcccc ggtcaactgg acg
//
LOCUS       DASZAK010000267         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_397428, whole genome shotgun sequence.
ACCESSION   DASZAK010000267 DASZAK010000000
VERSION     DASZAK010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397428"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..>2274)
                     /locus_tag="VGT44_04635"
     CDS             complement(293..>2274)
                     /locus_tag="VGT44_04635"
                     /inference="COORDINATES: protein motif:HMM:NF033527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEV2580120.1"
                     /translation="QGGDPVQALLDTVPEAELAATVESAKQLLRPEDLDSLDLISTRY
                     APMRKSLLLLYQALDFQPFRRAEPALQALNHVSLLAKQHRRVTAKEQRVGKVKVKAPL
                     AHLTPRWRKHALKGDKITPNYYEAAAFEALKGRVRSGDVAVSGSRRYRSFEHYLLPKT
                     QFEQLSHKQQTRLTVSDDAAAYLAEKQQEIRQKLAALQESIGKIEGSLTLDDKGHLHL
                     PSLDKAVPEDVERLKKRVYTLLPHLPLADLLLEVDNWTGFLRHFTHLTSGDAPLGGQK
                     LELVAALMGMGMNLGLEKMAESCPYSYRQLAWCIDWHIREETLLSALGKLDDFVLSAP
                     LSRAWGDGTTSSSDGMRLHVGVNAANAEYNAKHFGVGRGTNMYIHTADIWVPFGKPQI
                     IGTNEEALYVIDALCHHESDLHIREHYTDTGGSTEQVFALASLLGFRFAPRIADALAK
                     KLYMLDEVGGTEQLHTLVFDQVNKKLIIEQWDEMRRVASSIRHGTVSASLLMRKLAAY
                     PRQNQVARALTELGKLEQTAFLLEYFRDSALRRRILIGLNTGEALHALARQLFFGRLG
                     ELRDRALEDQIHRASCLHLIMAAIAAWNTVYLDVAIATLRKRGEDIPEATVAHIAPLG
                     WEHIRLIGDYHFAPQTGRSLDNLRTLRLTEAQESA"
BASE COUNT          450 a          665 c          659 g          500 t
ORIGIN      
        1 ctggacttcg ttgttgtaga tttaagattc gacttttctc tcaactggaa ataggagaac
       61 agagagaata tacagcattc cttttccgac cgcaagtact gaatcttctt ctttcctttt
      121 tccatatagc taagtccaaa aaaccatcgt ttcaaagaca acttagcagt tggatacctg
      181 cattccagct tcatattact ccaggacagc cgacaggaca ctccggtatc acagcaccat
      241 ctgcttcgct ctgtgtgtga gcgagcggag tgcgtgtttc tcctcttgct tttcaggcgc
      301 tttcttgcgc ctccgtcagg cgcagggtgc gaagattgtc caacgagcgc cccgtttgcg
      361 gggcgaaatg atagtcgccg atcaagcgga tgtgttccca tcccagcggg gcaatgtgag
      421 ccacggtggc ttccgggatg tcttctccgc gcttgcgcag ggtcgcgatg gcgacgtcga
      481 ggtagacggt attccaggcg gcaatggccg ccatgatcag atggagacaa ctggcgcgat
      541 ggatttgatc ctccagggca cgatcgcgca gctcccccag gcggccaaag aagagttggc
      601 gggcgagcgc atgcagcgct tcccctgtgt tgaggccgat caggatgcgc cggcgcagtg
      661 cagagtcgcg gaagtactcc aaaaggaacg cggtctgttc cagcttgccc aactcggtga
      721 gggcccgggc gacctgattc tggcgcgggt aggctgccag cttgcgcatc agcaaggagg
      781 ctgacaccgt gccatgacgg atcgaggagg ccacccgccg catttcgtcc cattgctcga
      841 tgatcagttt cttgttgacc tggtcaaaga ccaatgtgtg gagctgctca gttcctccca
      901 cctcatccag catatagagt ttcttggcga gggcatcggc aatgcgcggc gcgaagcgga
      961 aacccagtag tgacgccaga gcaaacacct gttcggtact tcctccggta tcggtgtaat
     1021 gctcgcggat atgcaggtcg ctctcatgat ggcagagggc atcgatcaca tagagggctt
     1081 cctcgttggt cccaataatc tgcggcttgc caaacggaac ccagatatcg gccgtatgaa
     1141 tatacatgtt ggtgccccgt ccgaccccaa agtgtttggc attgtactcg gcgttggcgg
     1201 cattgacccc gacgtgcagg cgcatcccat cagaagacga ggtcgttcca tctccccagg
     1261 cccgcgagag cggagccgag agcacaaagt cgtcgagctt gcccagcgcg gaaagcaagg
     1321 tctcctcccg gatgtgccag tcaatacacc aggccaactg tcgataggaa tacggacagg
     1381 actccgccat cttctccagt cccaagttca tccccatgcc catcagcgcg gcgaccagtt
     1441 ccagcttctg tccaccgaga ggcgcatcgc cactggtcag atgggtgaag tggcgtaaaa
     1501 agcccgtcca gttgtcgacc tccagcagca gatccgccag cggcaggtga ggcaagaggg
     1561 tatagacgcg ctttttgagg cgctcgacgt cttcggggac ggctttgtcc agggacggaa
     1621 ggtgcaggtg ccctttgtca tccaaggtca ggctcccttc gatcttcccg atgctctctt
     1681 gtaaggccgc cagtttctgg cgaatctcct gctgcttctc tgccaggtag gctgctgcat
     1741 cgtcgctcac tgtgaggcgg gtttgctgct tgtggctgag ctgttcaaat tgcgtcttcg
     1801 gcaaaagata gtgttcaaac gagcgatagc gcctgctgcc actcaccgcc acatctcctg
     1861 agcgcacccg ccctttgagt gcctcgaagg cggccgcttc gtagtaattg ggcgtaatct
     1921 tgtctccctt gagcgcatgc ttgcgccagc gcggtgtcag atgtgcgagt ggggctttca
     1981 ccttcacctt gcccacccgt tgctcttttg ccgtcacccg gcggtgctgc ttggccaaca
     2041 gggaaacatg gttcagcgcc tgtaacgcgg gttcagctct acgaaagggc tgaaaatcga
     2101 gagcctgata cagcaagagc aagctcttgc gcatcggcgc gtagcgcgtc gagatcaggt
     2161 cgagcgaatc caggtcctct ggccggagca gctgtttggc cgactcgacc gtcgcggcaa
     2221 gttcggcctc tggcacggtg tccagcagcg cctgaacggg atcgcccccc tgcg
//
LOCUS       DASZAK010000268         3679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_362297, whole genome shotgun sequence.
ACCESSION   DASZAK010000268 DASZAK010000000
VERSION     DASZAK010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362297"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VGT44_04640"
     CDS             <1..805
                     /locus_tag="VGT44_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2580121.1"
                     /translation="SPDPTHAAFVHECLRQHKPVLCEKPLTTTAAEAQKLIEAEQVLG
                     QRLISVGFNRRFDPRHLAVRQVVAEGEIGRAILYKGVHRNATIPPYSPGESVVTGSAI
                     HDIDSIRWLLGQEITEVYVRGVRTHSSFSDETLDMLLLQMVVSGDCLATVEVSVAIEY
                     GYEVSGEIVGERGTALTTQPGLALVRSREARSVAVNTDGLVYFHESYVSELMHWVQAL
                     QTRQAFTGASAWDGYMSLVVSDACIQSLHRGTPVPVPTRERPGFYQERV"
     gene            899..1636
                     /locus_tag="VGT44_04645"
     CDS             899..1636
                     /locus_tag="VGT44_04645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEV2580122.1"
                     /translation="MTDVTITTPTHQLRGYFARPVGEGPWPGVIVIHDAAGMSNDLRH
                     QADWLAGAGYLAVAPDLLSWGRKLACLVAVFRDLQAGRGQSFADIEATRQWLVGQASC
                     SGRIGVIGFCLGGGFALMLASDSAFAASSVNYGVTAYTEAALRGACPIVGSFGSKDRN
                     LPGAAQKLDQALETLGIDHDVKEYPQAGHSFLNDHRREGPWGTLYVVLGHLPVIAGYV
                     GAYHEPSAQDARQRILSFFNRHLQAGS"
     gene            1883..2470
                     /locus_tag="VGT44_04650"
     CDS             1883..2470
                     /locus_tag="VGT44_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580123.1"
                     /translation="MSRMLSSKGNDETALKVTAIPTAYKNEEEHAMKTVGEKIDKEVL
                     TIGQRVGELISVVLMLLLFSFFVYHQVAQTGFFTAKFGALEMFCLYGPILLSLVAPVV
                     RAVSGRRNPARPFDVATNVFLAIAAIWLLMVFPFNFAHLADPLPGAFQFVLAWITNDI
                     GRVFLILQIIIGSITAILTTWKYLSMRRREFAHVW"
     gene            2662..3441
                     /locus_tag="VGT44_04655"
     CDS             2662..3441
                     /locus_tag="VGT44_04655"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEV2580124.1"
                     /translation="MTTLQIERSSGEAVVGPDPSWRGLYRSGGVSAVLFVVLTIVSLV
                     LAFVTPQPQSSGGSLMLPGGVATLQFIASNRSVFILDQILFVGPVVLTIVVFLALYVA
                     LKHLSKSYAAIGAVFGIVGVVLSLVPFSFVGGLVYLSDQYAAATTVAQRAVFETAADS
                     LIAQNNIVSAGGILFAVGVLVISLAMLKGVFHKGIAYLGIVTGVVGIICESLRPILGP
                     GYSLYLVLLIWLSVVGWTLYRLGSSSSTSRVARQLEAQGAP"
BASE COUNT          766 a         1009 c         1070 g          834 t
ORIGIN      
        1 atcacccgat ccaacccatg cagcctttgt gcatgagtgc ttgcggcagc acaagcctgt
       61 actctgcgaa aaacctctga caaccactgc cgctgaagct cagaagctca tcgaagcgga
      121 acaggtcctg gggcagcggc tcatctctgt cggctttaac cgccgctttg atccccggca
      181 cctggctgtc cggcaggtgg ttgccgaggg ggaaattggc cgcgcgatcc tgtataaggg
      241 tgtgcatcgc aatgcgacaa tcccgcccta ttcccctggc gagtccgttg tcacaggttc
      301 cgccattcac gatattgatt cgatacgctg gctcctgggg caggagatta cagaggtgta
      361 tgtgcgtggg gtgcgaacgc attcctcgtt cagcgacgag acactggata tgctactcct
      421 gcaaatggtg gtgagcggag attgcttagc aacggtggag gtttctgtcg ctattgagta
      481 cggctatgag gtgtcgggcg agatcgtcgg tgaacgaggc actgctctga ccacgcagcc
      541 tggtcttgcg ctggtgcgat cccgagaagc acggtcggtg gctgttaaca ctgacggcct
      601 cgtgtacttt cacgaatcct atgtctccga gttgatgcat tgggtgcagg cgctccaaac
      661 caggcaagcg tttaccggtg cgtcagcctg ggatggctat atgtcgctgg tggtcagcga
      721 tgcctgcatc caatccttac acagagggac gccagtaccg gtgccgacgc gggagagacc
      781 aggtttctat caggaacggg tgtgacaaga agcaatattt gtcaaacgag aaatgcattg
      841 gaagaaccac ccggtgatga cgagcgctgg tggttcaatc agtcagaaag ggactaacat
      901 gacagacgtg accatcacaa ccccaacaca ccagttgcgc ggctacttcg cccggccggt
      961 gggtgagggg ccgtggcccg gcgtgatcgt gattcatgat gccgccggca tgagcaacga
     1021 cctgcgccac caggcggact ggcttgccgg tgccggctat cttgccgtgg cccccgacct
     1081 cttatcctgg gggcggaagt tggcctgcct ggtggcagtc ttccgcgatt tgcaagccgg
     1141 tcggggtcag tccttcgcag acatcgaggc cacccgccag tggctggtcg ggcaagcgag
     1201 ctgcagcggg cgcatcgggg tgatcggctt ctgcctgggc ggtgggttcg ccctgatgtt
     1261 ggcctccgat agcgccttcg cggcatcgag tgtgaactac ggcgtgacag cgtacacgga
     1321 agccgccttg cgcggtgcct gcccgatcgt cggcagcttc ggctccaagg atcgcaacct
     1381 gcccggagcc gcccagaagc tcgaccaggc cctggagaca ctcgggattg atcacgatgt
     1441 caaagagtac ccacaggccg ggcactcctt cctcaacgac caccgcaggg agggcccgtg
     1501 gggcaccctg tacgtggtgc tgggacacct cccggtgatc gcaggctatg tcggcgccta
     1561 ccatgaaccc tcagcacagg atgcccgcca gcgcatcctt tcgttcttca accgccatct
     1621 ccaggcgggt tcctgactgc tggcgtgaag agagacgggg agtgggagag caaagcacag
     1681 gcgtgatccc aaacctcctt cttctcttga ccgctgcata agccaggcac cttcagcgcc
     1741 tcaactgaaa aacactccta tcgtcgtcgt tcggcgggcg accacacgca tctccccgat
     1801 gcaccatgcc atgcccggtt caagaacgga ttgtgatgcg agtgagatac cagaaccggt
     1861 ggccgtgagg caggtgtgat cggtgagtcg tatgctttca tcgaagggaa atgatgaaac
     1921 tgctctaaaa gtgacagcga taccaacagc atacaaaaat gaggaggagc atgcgatgaa
     1981 aacggtagga gagaaaatcg ataaagaagt ccttactata gggcaacgag tgggagagct
     2041 catcagcgtc gtcctaatgc ttctgttatt tagtttcttc gtatatcatc aagtagcaca
     2101 gacaggcttt ttcaccgcca aattcggagc gttggagatg ttctgcttgt atggccccat
     2161 ccttctttct ttagtcgcac ctgtcgttcg tgccgtgagc ggtcgtcgca atccagcccg
     2221 cccattcgat gtcgctacga atgtgttcct ggcgattgct gctatctggc tgttgatggt
     2281 ctttccattt aacttcgctc atctcgctga ccccttgccc ggcgcatttc aatttgtcct
     2341 tgcctggatc accaacgata ttggcagagt attcttgatt ctccagatca tcattggctc
     2401 aattacagcc atcttgacga cgtggaaata cctatcaatg cgccgacgag agtttgcaca
     2461 cgtatggtaa cagcaggcat cggagctgct agtcatttcg acatgactgt cattcagcag
     2521 aagccgtaga ttgactcaca gggggcttca cctgatgtct ggtctcccac acgaaggtgt
     2581 cagagaaggt gcagatttga tagaacggaa tctaaagaag agtgagaagt acaatacgca
     2641 attccgcaaa ggaggaaaga tatgacaacg ttgcaaatag aaagatcatc gggagaggct
     2701 gttgttggtc cagacccatc gtggagaggt ctctatcggt ccggaggcgt ttcagcagtg
     2761 ctctttgtcg tgctgaccat cgtatcatta gtgctggcct tcgtcacgcc gcaaccgcag
     2821 agctcgggcg ggtcgctcat gctcccgggt ggggttgcca ccctccagtt catcgcctca
     2881 aataggtcag tcttcatcct cgaccaaatt ttgtttgtgg gacccgtggt cttgaccatt
     2941 gtagtcttcc tggctctcta cgtggcgctc aagcacctga gcaagagcta tgctgcaata
     3001 ggcgccgtct tcgggatcgt tggcgtggtt ctctcgctgg tcccgttttc gttcgtgggc
     3061 ggcctggtgt acctgagcga tcaatacgcg gcagccacca ctgtcgcaca acgtgccgtc
     3121 ttcgagactg ctgctgatag tcttattgca cagaataaca tcgtgagtgc cggtggtatc
     3181 ttgttcgcgg tcggcgttct ggtcatctct ctcgcgatgt tgaaaggcgt cttccataag
     3241 ggcattgcct atttaggcat agtcactggc gtcgtaggga tcatttgtga gagccttcgg
     3301 cccattcttg gccccggcta cagtctctac cttgtcctcc tgatctggtt aagcgtggtc
     3361 ggctggacac tctaccggct gggatcgagc agttcgacgt cgcgtgtggc gaggcagttg
     3421 gaggctcagg gggcaccgta gcagcggctt cagcaaaagc agcaagaaga tgaaacacat
     3481 actggtccgt cttactatcg gagacctgaa aagaaagaag ggacacgttc atggctaaaa
     3541 cctatcacgt caattttgct gaccgcgtgg aaaacgcact gatcatgccg ctgctgcgtg
     3601 ctggcgtcaa gatggggacg acgtcgctat tgaccgttcg cggccgcacc aggggtcagc
     3661 ctcatacgat tccggtgat
//
LOCUS       DASZAK010000269         4592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_255864, whole genome shotgun sequence.
ACCESSION   DASZAK010000269 DASZAK010000000
VERSION     DASZAK010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255864"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VGT44_04660"
     CDS             complement(<1..1134)
                     /locus_tag="VGT44_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580125.1"
                     /translation="MKQVSLRAVNRPSPPIADTRLSGTRLIIARSVWIVLMFLSCGFY
                     LAAVLVYYVQLHGLQEGVYAHLISTPAVVSGYNAFESSSTLFTGPYATLNITLITLVL
                     PLFWIAVSLVIFWRRSDDWMALFVALFLVMLVTIFSPALSALSYVIGYTSPLGMCITL
                     LQLLAMSSFGFFIALFPDGRFVPGWTRWLTLTYLLVLVFLCLPSTSPYSMVRWPPVLL
                     GPLLLGLAFAWGFAQLYRYRRVSTTIQRLQTKWIVFGMLVAMLWDVSNVLLPLVLPGS
                     AHPVFIALSEVTFPLAFLVIPGTIGFAVLRYRLWDIDLIINRTLVYGLLTLSVLSLYI
                     PVVVGLGTVFSALGNLFLSLLATGLVAVLVQPLRQRLQRAVNHL"
     gene            complement(1519..2754)
                     /locus_tag="VGT44_04665"
     CDS             complement(1519..2754)
                     /locus_tag="VGT44_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV2580126.1"
                     /translation="MMTITNKNILISGASIAGPTLAYWLKRYGFTPTVIERAPHLREG
                     GYPVDVRFEAVQVAKLMGIWPRLQQEQTTLEEMSFVNERHQRISRVNNMQALPKALGV
                     DVVEIMRGDLARTLYELTKDEVEYCFGDSIQALKQDEAGVDVTFERGGSRRFDLLVGA
                     DGLHSIVRTLAFGDEAQFERYYGYHVGVFTTDNYLGLDEACMQMYNVPGKAVAVRTSR
                     GNRALVAYFLFKQPTRLHYDYHDVERQKQLLASVFAGEAWEVPRLLEKMQTASDLYFD
                     AVSQIRMETWSHGRVVLLGDAGYCPALLSGQGSTLAMMGAYILAGELKAALGEHQRAF
                     HQYEQAFRPVVRQEQKKVGSGKFLVPATRLGLWVRNHLFPLLVPPMLVAIERGRRLLP
                     RSYSRPSRLKDYGDVLPPS"
     gene            complement(2799..4439)
                     /locus_tag="VGT44_04670"
     CDS             complement(2799..4439)
                     /locus_tag="VGT44_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2580127.1"
                     /translation="MMFHSILSKSLRDYRVPMLAWGFGLLVFMATFVAMATPAEVMAY
                     ISLVKILSFWGDPYAINTPEGYITFRWMETGWPILLSIWPILAGARLVRGEEERGTMD
                     VLLATSQTRTRLLLEKVGALVIALLVIAVLFALGVVAGEAALGGGHVDVVRALLAGLN
                     LVLLAFFFGMVALLLSQLTTGRRAAAGWTSGLLILFVFLNMAGRELNGAWVQYLSPVY
                     YYNLNRPLLAGFPDQPLAALVPLGLAVLCLGGSVFLFARRDIGRSAIESQREVASGSQ
                     QTLRSLSRAEREVSTRAVGLQTLFADGWGAFWWLLGIVAFCALWQFLTPGLQKFFNQV
                     VQQTPWLARYLFDTPTNTNTAVLGTIVFAFAPALVVILALTLALKWPSDLENGRLELI
                     FSTPQSRPRVLLERFGANALIVLLAAVVIWLTLTIGAQLANLSINQGRLLAASFSMLP
                     PALITMVLVYTLAGRLRYGAVLGIATAYLVLSYLQESLEGNIQFPGWVMSLSVFHLYG
                     DPIFLGMNWTNFLGMTGVAFALLVISLLQFRYIDIELG"
BASE COUNT         1054 a         1375 c         1359 g          804 t
ORIGIN      
        1 caggtggttg acggcccgtt gcagccgctg gcgcagtggt tgcacaagca cagcgaccag
       61 gccggtagcc aacagggaga ggaacagatt ccccagcgcc gagaacactg tgcccaggcc
      121 gacgactacc gggatataga ggctgagcac gctcagagtg agcagtccgt agacgagcgt
      181 gcgattgatg atgagatcga tgtcccacaa ccggtaacgc agcaccgcaa aaccaatcgt
      241 gccggggata acgagaaaag cgagggggaa ggtcacttcg gagagtgcga tgaaaacagg
      301 gtgagcagag ccaggaagga cgagcggaag cagcacatta gagacatccc agagcatagc
      361 caccagcatg ccgaacacga tccacttggt ctgcagccgc tggatggtcg tggagacgcg
      421 ccggtagcgg tagagttggg cgaagcccca ggcgaaggcc aggcccagca agagagggcc
      481 gagcagaaca ggtggccagc gcaccataga gtagggcgag gtcgacggca ggcacagaaa
      541 caccagcacc agcaggtagg ttagggtgag ccagcgcgtc caaccaggga caaagcgccc
      601 atcggggaag agcgcgatga agaagccgaa cgaactcatg gccagaagtt ggagcagggt
      661 gatgcacatc cccaggggag aggtgtagcc aatcacatag gagagcgccg agagggcggg
      721 tgagaaaatg gtcaccagca tcaccaggaa caacgcgacg aagagcgcca tccagtcgtc
      781 ggaccggcgc cagaagatca ccaggctcac tgcgatccag aacagcggca agacgagcgt
      841 gatgagcgtg atgttgagcg tggcataggg gccggtgaac agcgtggagg aggactcaaa
      901 ggcgttgtag ccactgacca cggcaggcgt tgagatgagg tgggcataca ctccttcctg
      961 gagcccgtgg agctgcacat agtacaccag cacagctgcg aggtaaaacc cgcaggagag
     1021 gaacatcaac acgatccaca ccgatctggc aatgatgagt cgcgtcccag acaggcgggt
     1081 gtctgcgatg ggtggcgaag gcctattgac ggcacgcagc gatacctgct tcacgaatgt
     1141 tcctcctgtt tcttgctcgc gcgagttcgc gctccatatc ttgcgcacat gtattgtacc
     1201 tcgaacgggg cgttcgtgcc cagcagaaag gcccatgtag ctcgacgctc caaaatgtac
     1261 aacgttacgc tcttcatcag caagacctcg ccagctcatg aggagggctg tatcgaatct
     1321 cacttgccct caaccattgt ctccgcgacg actggtcgca tcttttgtct tttcgtactt
     1381 cttgttcatt ctcaacacca gcttgccgtt gtctttttca tttgcttcaa aaaggtggtg
     1441 caagttgggg ctgagacagc gagctacgat gtgttctgga catcgcctcc acgccgatcc
     1501 caccgcagca caccttcgtt aagacggcgg gagcacatcc ccatagtcct ttaacctgct
     1561 gggccttgag taggacctgg ggagcaatct cctcccgcgc tcgatcgcca ccagcatagg
     1621 cggcacgagc agcgggaaca agtgatttcg cacccagagg cccaaacgcg ttgcgggaac
     1681 caggaacttg cctgatccga cctttttttg ctcctgtctg accacgggcc tgaaggcctg
     1741 ttcgtactga tgaaacgctc gctgatgctc gcccagcgcc gctttcagtt ctcccgccag
     1801 gatataggct cccatcatgg ccagggtcga cccctgccca gagagcagtg ccgggcagta
     1861 gccggcatct cccagcagca ccactcgtcc gtgagaccag gtctccatcc gaatctggct
     1921 caccgcatca aagtacaagt cggaggccgt ttgcattttc tccaggagtc tggggacttc
     1981 ccaggcttcg ccggcaaaca ccgaagcgag cagttgcttt tgccgctcca catcatggta
     2041 atcgtagtgt aacctggtgg gttgcttgaa caggaaatac gcgaccagcg cccggttacc
     2101 ccttgacgtg cgtaccgcga ccgcttttcc agggacattg tacatctgca tgcaggcctc
     2161 gtcgagtcct agatagttgt cggtggtgaa gaccccgaca tgatagccgt aatagcgctc
     2221 aaattgcgct tcgtctccaa acgccagggt ccgcacgatc gaatgcaacc cgtcagcccc
     2281 caccagcagg tcgaagcggc gggagccgcc ccgctcaaag gtcacgtcga ctcctgcctc
     2341 gtcctgcttg agcgcttgga tggagtctcc gaagcagtat tccacctcgt ccttggtgag
     2401 ctcgtagagc gtcctggcga gatcgccccg catgatctcc accacatcca cgcccaaggc
     2461 cttgggcaga gcttgcatgt tgttgacgcg gctgatgcgc tgatggcgct cgttgacaaa
     2521 cgacatctcc tcgagggtcg tctgctcttg ttgcagacgc ggccagatcc ccatcagctt
     2581 ggccacctgc acggcctcaa agcgcacgtc aaccgggtac ccgccttctc gcaggtgtgg
     2641 cgcacgctcg atcacggtcg gggtaaaccc atagcgcttg agccaatacg ccagggtcgg
     2701 cccagcaata ctggctcccg aaatgagaat gtttttgttg gtgatggtca tcatggcttt
     2761 cactcctctt cattgctcgt gctgctcctg aagcttgctt agccgagctc tatatcgata
     2821 taacggaact ggagcaggct aatcaccagc agtgcgaaag ctacgcctgt catgccaagg
     2881 aagttggtcc agttcatgcc gaggaagatc gggtcgccgt acaggtggaa caccgagagt
     2941 gacatcaccc atccaggaaa ttgaatattc ccttcgagcg attcttgcag gtaagacagc
     3001 acgagatagg cagtcgcgat gcccagtacg gcgccatagc gcaggcgtcc ggccagggta
     3061 tagaccagga ccatggtaat cagcgccgga gggagcatgc tgaagctggc ggcgagcagt
     3121 ctgccctgat tgatggaaag attggcgagc tgcgccccaa tggtaagggt gagccagatc
     3181 acgacagcag cgagcaggac gatcagggca ttcgcgccga agcgttccag cagaacccgt
     3241 ggccgcgact gcggggtgct gaaaatcagt tccaggcgcc cattttccag gtccgagggc
     3301 cacttcaagg cgagcgtcag cgcaaggatc acgaccagcg ctggcgcgaa ggcgaagacg
     3361 atggtaccca gcaccgctgt atttgtgttg gtgggggtgt caaacaggta cctggccagc
     3421 cagggggtct gctgcactac ctggttaaag aacttctgta agccgggagt caggaactgc
     3481 caaagggcac aaaaggccac gatgccgagc agccaccaga aggcacccca cccatctgcg
     3541 aagagcgtct gcaagccgac ggcacgcgta gagacttcgc gctcggctcg gctcaggctg
     3601 cgcagcgttt gctgtgagcc gcttgccacc tcgcgctgcg actctatcgc cgagcgtccg
     3661 atatcacgcc tggcgaacag gaacacactt ccccccaggc acagcacggc gagacccagc
     3721 gggaccagcg ccgccagcgg ctggtcaggg aaaccggcaa gcagcggccg gttgaggtta
     3781 taataataga ccggcgagag atactgcacc caggcgccgt tcagttcccg tccggccata
     3841 ttcaagaaca caaacaggat caacagcccg cttgtccagc ctgctgccgc gcggcggccg
     3901 gtcgtcaatt gagagagcag cagcgccacc atgccaaaga agaaggccag caagaccaga
     3961 ttcagccctg ccagcagcgc cctgacaaca tcgacgtgcc caccgccaag agcagcttca
     4021 ccggccacga cccccagcgc gaacagcacc gcaatcacga gcagcgcgat caccagcgcg
     4081 cccaccttct ccagcagcag gcgcgtgcgc gtctgtgacg tggcgagtag tacatccatg
     4141 gtgccgcgtt cctcttcccc gcgcaccagg cgagcgccag ccaggatggg ccagatgctg
     4201 agtaagatcg gccagccggt ttccatccag cgaaaggtga tatacccctc gggggtattg
     4261 atcgcatagg gatcgcccca aaagctgagg attttcacta agctgatata agccatcacc
     4321 tccgccggtg tggccatggc tacaaaggtg gccatgaaga caagcagtcc aaagccccag
     4381 gccagcatcg gaacccggta atctcgaagc gacttactca agatgctgtg aaacatcata
     4441 cacctcctat ccggcaagcg cggaagactc ctgctgcggc tcatagaagc gcaggaaggc
     4501 ttcttctagc gaaggctcgt gggtcgcaat attcgtggcg ccgtgctggc ccgcgatctg
     4561 gatgatctcc gccaagtcgc cctggacgac ga
//
LOCUS       DASZAK010000270         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_6803, whole genome shotgun sequence.
ACCESSION   DASZAK010000270 DASZAK010000000
VERSION     DASZAK010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6803"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..1639)
                     /locus_tag="VGT44_04675"
     CDS             complement(74..1639)
                     /locus_tag="VGT44_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV2580128.1"
                     /translation="MATETKSSAPVEMVPTLGLTGVTINAMALIAPGAFLWTTYESQA
                     APSSAINMWFAVLVATAIALMTAVAYASLSKRYPEGGAGSSYYYAEAAVLQQEEHRHF
                     KWARFAKFLVGWASHIYYWVYPGVMVAFMGIVITYIIQTFVPSFGAAWQEIAVCVIFA
                     AIVGGIAYIGVQGATVVNIILNVVQIVGLLVFSILAIVYRVTHPAVQYLHPNALSVIL
                     PHDLGGLVFQSTIAILLVVGFESATALAAEAKNPGRDIPRGVILSLIIQAVIFYLFEY
                     FAANFVINNGTKFTGTTSSGFAGAFASGAPIGDIATQIGNTLLGGNGTALAVILACGV
                     VAALVGTSLSCLNTGVRVTYAMGKDTELPVVFGFLHGKYRTPHVAVIVLTVLSALIGA
                     YGVLSIDNLTQVVLISNIGTFILYGMTCIVCILAFVGVKGRNWFSTIVAPLLGAIMNI
                     LMLIGVAYFDLFTQGSSYTRDTSIAIGFALAWLVIGFAFLYFRKVVSGVAILHPEDHK
                     EKVAATAGAAGGD"
     gene            complement(2118..>2748)
                     /locus_tag="VGT44_04680"
     CDS             complement(2118..>2748)
                     /locus_tag="VGT44_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2580129.1"
                     /translation="IAGVLLQAQGPAAVLFAALWLGERLTWLQIGGSALLLCGSVLVV
                     VQGGPALTWQGAGIGVLFVLAGAIGYGFSLIPAKRLAAQADPLQVSALRLLLGAIFVL
                     PFLFFQSPLVVGTVSWLLGWLFGAYIVTNFCIGYITQQTGLRFLKAWEAAAIMQTVPL
                     FTALFALVVLHETVTLLQLLGGVLVIVGGIIVARGETGISLQAPGRPQG"
BASE COUNT          719 a          758 c          754 g          517 t
ORIGIN      
        1 accatgaggg gcattgtgtg tctctacccg gtgatgtgct ctttcaagta ccgggaaacc
       61 tgtttaggat gggttagtct cccccggctg ctccagcagt tgccgcgacc ttctccttat
      121 gatcctccgg gtgcaggatc gccacgccgc tgactacctt gcggaagtag aggaaggcga
      181 agcctattac cagccaggcc agggcaaaac caatcgcgat agacgtgtcg cgtgtgtagg
      241 aggagccttg cgtgaaaaga tcgaagtagg ccacaccgat cagcatcagg atgttcatta
      301 tggcccccag caaaggagcg acgatggtcg aaaaccagtt gcgccctttg acgcctacaa
      361 aggccaggat acagacgata caggtcatac cgtagaggat gaacgtaccg atgttggaga
      421 ttagtaccac ctgagtcagg ttatcgatgc tgagtacgcc atacgcgccg atcagcgccg
      481 agaggacggt cagcacgatc acagccacgt gcggcgtgcg gtatttgccg tgcaggaagc
      541 caaagacgac cggcagttcg gtatctttgc ccatggcata agtcacacgc acgccggtat
      601 tcaagcagga aagcgaggtg ccgaccaaag cggctacaac gccacaggcc aaaatcaccg
      661 ccagggcagt accgttaccc cccagaagcg tattgccgat ctgtgtagcg atgtcgccaa
      721 tgggcgcgcc tgaagcgaat gcgcccgcga agccggaact ggtcgtaccg gtgaatttcg
      781 tgccgttgtt gatgacaaag ttggccgcga agtattcaaa gaggtagaaa atgaccgcct
      841 ggataatcag cgagagaatc acaccgcgag gaatgtcgcg gccagggttc ttcgcttcag
      901 ccgcaagcgc tgtcgcggac tcaaacccga ccaccagcaa gatagcaatc gtggattgga
      961 agaccaggcc gcccaggtcg tgcggcagga ttacgctcag cgcgttggga tgcaggtatt
     1021 gtaccgcagg atgcgtgacg cgatagacaa tggccaggat tgagaagacc agcagaccta
     1081 caatctgaac gacgttgagg atgatgttca ccaccgtcgc gccctgcaca ccgatatagg
     1141 cgatgccgcc cacaattgcg gcgaagatta cgcacaccgc aatttcctgc caggccgcgc
     1201 caaagctggg aacaaaagtc tggatgatat acgtaatgac gatacccatg aatgcgacca
     1261 tcacaccggg atacacccaa taataaatgt gggaggccca accaacaaga aacttggcaa
     1321 aacgcgccca cttaaaatga cgatgttctt cctgctgcaa aactgctgcc tcggcataat
     1381 agtaggaact gcccgcaccg ccttcgggat agcgcttgga gagactcgca taggcaaccg
     1441 cggtcatcag ggcgatagca gtagcgacta agacggcgaa ccacatattg atagccgagc
     1501 tgggtgcggc ctgcgactca taggtggtcc agaggaaagc gccaggcgcg atcagcgcca
     1561 tagcattgat ggtaacgccc gtcaaaccca atgtgggaac catttcgacg ggggccgaac
     1621 tttttgtttc agtggccaaa aggagactcc tctcttcgta cacggaacac tgcacaacat
     1681 caacaacaat ggcatcacta ccacagcttg tagccagcgc tgcatacgca tagaggccga
     1741 ggcgcactcg tcgcgcactc tatcatctct cgtgggaagt ataagaaata atggtgatgt
     1801 aaaagaactg tcaaaatgga agaagattgt gtcaaaaatt tgtgcatagt atacaaagag
     1861 gtggttattc ggcaacgact cttgctgtta agaggccatt cattattcgt attttggatt
     1921 gtatcatgcg tttctttgta atttgtacaa aaccccaggg agaaccttgc gtcttacatc
     1981 ggtaggtttt ttagaaaatg aaccagcgag gtaggaggtg tcatcctgag cgcagcgaag
     2041 gatctcggtc tggcccgcga gagatccttc gctgcgctca ggatgacagc ctctggcgat
     2101 cgctcgtttc taaaacccta cccttgcggt cgccctgggg cttgtagact aataccagtt
     2161 tcccctcgcg ctacgatgat cccgcccaca attaccagga caccacccag caattgaagc
     2221 agtgtgaccg tttcatgcaa aacaaccaga gcaaagagcg ccgtgaacag cggtacggtc
     2281 tgcatgatgg ctgccgcttc ccaggccttc aggaaacgca gtccggtctg ttgcgtgatg
     2341 tacccgatgc aaaagttcgt gacgatatag gccccaaata gccagcccaa cagccaggaa
     2401 acggtcccaa caacaagagg cgattggaag aagaggaagg gcaacacgaa gatggcgccc
     2461 aagagcaggc gtagggcgct gacttgcaac ggatcagcct gggcagccag tcgctttgcc
     2521 gggataaggc taaaaccata tcctatggcc ccggccagta caaacagcac accaatgcca
     2581 gcaccctgcc atgtcaaggc gggcccgccc tgcacaacga ccagcacgct gccgcaaagg
     2641 agcagcgcgc ttccaccgat ctgcaaccag gtgagccgct cgcctaacca gagtgccgca
     2701 aatagaactg ctgccggtcc ctgagcctgg agcagcactc ccgctata
//
LOCUS       DASZAK010000271         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_574977, whole genome shotgun sequence.
ACCESSION   DASZAK010000271 DASZAK010000000
VERSION     DASZAK010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_574977"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VGT44_04685"
     CDS             complement(<1..1094)
                     /locus_tag="VGT44_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEV2580130.1"
                     /translation="MKVSSYRERDYAFGRVMLTLRTRLGLTQTELADMLGIRRRAVID
                     WEGGLTYPKADHLKQFVVLAVERQAFPAGREAEEIRALWQAARQKVLLDEVWLGGLLS
                     DAEASPASQSREETGITVHALASPSGGEQRVDWGDAPDVPSFYGREWELDLLSEWVVE
                     ERCRVVSVLGQGGIGKSALATKVMHRVAEDFEVVIWRSLRDVPTCEALLDDCLQVLAP
                     QALLDASSSLESRQSLLVDCLRSRRVLLVYDNLESFLEEGEDSGHIRPGYEGFARVLR
                     RVAETEHQSCWLLTSREKPADLVPLEGNRAPVRTLRLAQLDTEACRQLLAEKGAAGSV
                     AEHLRLIQAYAGNPLALKIVAQTIVDLFDG"
     gene            1386..>1712
                     /locus_tag="VGT44_04690"
     CDS             1386..>1712
                     /locus_tag="VGT44_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2580131.1"
                     /translation="MSNFASQRTSHKPSIVFAHGIWADGSCFSKVIPALQAEGYEVMS
                     SQHSLDTPEGDVATLIRTLGRVSSPAILVGHSYGGSVITAAGTDDRVAGLVYIAALAP
                     DAGETSQ"
BASE COUNT          356 a          543 c          502 g          311 t
ORIGIN      
        1 tggccatcga agaggtccac gatggtctgc gccacgattt tgagggccaa gggattgccg
       61 gcgtaggcct ggatcagccg gaggtgctca gcgacactcc ctgcggcccc cttttcggcc
      121 aacaactgcc ggcacgcttc cgtatccagc tgggccaggc gcagggtccg cacgggagcc
      181 cggttgcctt ccagcggcac caggtcggct ggtttctccc ggctggtcag cagccagcaa
      241 ctctgatgct cggtttccgc gacccggcgc agcacccgag caaacccctc gtagccggga
      301 cgtatgtgac cgctgtcctc cccttcctcc aagaacgact ccaggttgtc ataaaccagc
      361 agaacacgtc tgctgcgcaa gcagtccact aggaggctct ggcggctttc gaggctggaa
      421 gaggcatcga gcagcgcctg gggagccagc acctggaggc agtcatcgag cagcgcttcg
      481 caggtgggga catcgcgcaa ggagcgccag atgaccacct cgaagtcctc cgccacgcgg
      541 tgcatcacct tcgtcgccag cgctgacttg ccaatgccac cctggcccaa cacgcttacc
      601 actcggcatc gctcctcgac cacccattca gagagcaggt ctagttccca ctcgcgccca
      661 tagaagctcg gaacatccgg cgcgtcaccc caatcgacgc gttgctcgcc tccggaagga
      721 gaagcgagag cgtggactgt gataccggtc tcctctctgg actgagatgc cggggaagcc
      781 tctgcatcgg acagcagccc acctagccat acttcatcca ggagcacctt ctggcgggct
      841 gcttgccaga gagcacgaat ctcctctgct tcacgcccgg cggggaatgc ctggcgctca
      901 acggcgagga ccacgaactg tttcaggtgg tcggccttgg gataggtaag gcctccctcc
      961 caatcgatta cggcgcggcg tctgatgcca agcatgtcag ctagttcggt ttgcgtcagc
     1021 ccgagcctgg tacgcaaggt cagcattacc cgcccaaagg catagtctcg ctcgcggtac
     1081 gaggaaacct tcatagagct tccctttttg tctccttcac acagtaatgc aaagtaatgt
     1141 gcggtgataa ggtgtcgtga tgtccggtga cttcgagtac cctgatcagt atactacaga
     1201 acagagacga tcacctcaca gaagcagcaa ggtgacgatg agcagcggac tgacacacga
     1261 cctgaaaagt aaggcgtaac gcagaggtgc tgctcttgct gtcggcatga gatggatgcc
     1321 gcctcttgat aagcacaagg aagagcagtg ttgctgctca agaaatggaa aaaaggagaa
     1381 cacttatgag caattttgcg tcacaacgca cctcacacaa accgagcatc gtgttcgccc
     1441 acgggatttg ggccgacggc tcgtgcttta gcaaggtgat ccccgcgctt caggcggagg
     1501 gctatgaggt gatgtccagc cagcacagcc tcgacacgcc cgaaggcgac gtcgctacgc
     1561 tgatccgcac gctggggcgg gtcagcagtc cggctatcct cgtcggccac tcgtacggcg
     1621 gatccgtcat caccgccgcg ggaaccgatg accgcgttgc cgggctggtc tacatcgccg
     1681 cactcgcgcc ggacgcaggc gagacatcgc ag
//
LOCUS       DASZAK010000272         6274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_504145, whole genome shotgun sequence.
ACCESSION   DASZAK010000272 DASZAK010000000
VERSION     DASZAK010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504145"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2937)
                     /locus_tag="VGT44_04695"
     CDS             complement(<1..2937)
                     /locus_tag="VGT44_04695"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2580132.1"
                     /translation="MSEQLACQYCRYLLEGAVLGDCRVTRWIGSGAFGDVYEATQMPP
                     LSRRVAIKVMLPERVADEESAVLFEREVGAIAALDHPNILPVLRAGTLEDGRAYLVMK
                     YAAHGSLQQHCQLTPQGLSILPSARPINEEIETRVVIDEQVGGADKADGGDDGKEGNE
                     GRASEEGDSGVRDGDGSFDPAETVDMPVNEGQDERLAASSDALPRVSAGLTPTLTPQQ
                     LLPYVEDAAAALQYAHNHALIHLDVKPANLLLDGEDRLLLADFGVSTILDGYSHASLH
                     CFVGTPVYTAPEQWLEQPRPASDQYALAVTCYQLLAGRPPFTGSLYSIMHGHLQLPPP
                     PLRELNQLVPEQVETVILRGLAKEPSERYKDMRAFARAYRAAVEEAANSATDVQARTP
                     AKEATRPELPAVGEIVKQRGGKSDPATGPVTELLETRVLKQTTPLSRAAPYEVQSDKL
                     PSSPTRRKHGRVFAVLALVAVLLSGGTLGALRVFTPCLLGVCPAMRLSTPEVDITNAD
                     TQVVKITDSGTADLQWSVTNPVNDSWLAYSPTGGLLVPGQVAALTIKTNVDGLSNGVD
                     IAQLEINGQGVNALPLTVKLTVQTGLGQISAKAGNTQFIYDQEGVHPAAGTITITNKS
                     DQSFNWYIEYKDINNWLLVTPSTDTLAAGGTETLTVTANAQNLPPDTYTADFSILGSV
                     GQADPGILSDFRFTLEVRPSAQQGTPSVTVTTTATPTFPSVVLSALAPPSSNAPSTLR
                     SRHSMVWDSQDDLFFVFGGIDSQGNFLNDLWSYSPVTGLWSQIINAAATPVTGATCAG
                     GTWPAARADAAMVWDSAHSQILLYGGVGANTHYFGDLWSYSPASSAGNWTPLACSNNG
                     PGARASNAVWNGGQMLILGGSYRYGMLADFWTYTPGSNGTLGAWQRLTDFPGGQRAYQ
                     TMVWDTSDKMLLTFGGLDVNGLQRDDFYSYSTSGGWLPITPLSSSNPKPRQQGIGVWD
                     SKDRVML"
     gene            complement(3169..3579)
                     /locus_tag="VGT44_04700"
     CDS             complement(3169..3579)
                     /locus_tag="VGT44_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580133.1"
                     /translation="MSETFHELYASLLKDVFSHYARQQMEQGMDGLASARTYAEQGKP
                     DFALAFLLLIDIPDEEKRALLARAYEQRARHSDEKAAQLQTQFHRSFPMITLDAQKDR
                     AGAQAIRAGARVAGANDTGDEVGKGDHNEQSGEV"
     gene            3747..4406
                     /locus_tag="VGT44_04705"
     CDS             3747..4406
                     /locus_tag="VGT44_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEV2580134.1"
                     /translation="MRRNYWPIFFLLCLLTGCQSASQASQSSQLAPTRPSASSLFSTP
                     ISISGQPTPAKVSPTARSASQTATKSATGTPALTPTSQVQQLAQYVFGLINQSRAAQG
                     LRPYAWGGALAGGARLHNQRMIAYGQLSHQCPGEPALGARISADGIQWQAAGENIGWA
                     SYTNAQQGLLSIHQSMMAEQPPNDGHRQNILNTNYNLLGVDVVIDSKGQVWLTEDFAE
                     A"
     gene            complement(4412..6103)
                     /locus_tag="VGT44_04710"
     CDS             complement(4412..6103)
                     /locus_tag="VGT44_04710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2580135.1"
                     /translation="MSRVDEAQDAQRTTSPEGTRRAGRTRTGRGVPVFLRNPRAHTLI
                     AGLIVFGGALGFDLYRLGNPSVWFDEAFSVELAKQPLPLLWHIIWGPEPNMELYYLFL
                     HGWVGLTGLFGWLPTEFVVRLPSAIFAALAAVMVFLLGRCFVGMAAGIAGAGLFMLNY
                     LQLIYAQQARSYSLQLLLICLAWYALFAWLTTTGKGKRWWICFVLATTLAIYAQLFSM
                     LILMAQVVAFGGLLVLPGPWRERARQRLRGFGAALAAIGVLGIPMGLVSLHGAKTNWL
                     PIPQPNDILHLFQYITTNSPLFLMLLFLCCFCAVGIALLTILPEGKSVLQLVTFAGGE
                     NEQDESRLKQYFPVAFALVCWLFVPVIISYVVSQGSLRLFSSRYLVTILPPLFLLSGM
                     AIAALRWRIVQVGLIAGVLLIAVYYVPIYYRSAQVEDWNSAVPWLEQHYQSGDGLVCF
                     DNDVEQGCQIAVQYYLDAYPSAAHFTPDAPGAFSWEQFGPANPSNGTFAAVDPQALAA
                     YAAQHPRFFYILGRFPDNTSAARAQSAQDWLDSHYHLLGRIHAPTVTISLYGSGT"
BASE COUNT         1399 a         2002 c         1694 g         1179 t
ORIGIN      
        1 cagcatcaca cgatccttgc tatcccacac accgatcccc tgctggcgtg gtttgggatt
       61 tgagctgctc agcggcgtaa tcggcagcca gcccccgctc gtggaatacg agtagaaatc
      121 gtcacgctgc aacccgttca catccagacc gccaaaggtc aacagcatct tatcgctcgt
      181 atcccacacc atcgtttgat aagcgcgttg gccgccgggg aaatccgtca aacgctgcca
      241 cgcgcccagc gttccattgc tgccgggcgt atacgtccag aaatcggcca gcatcccgta
      301 gcgatatgac ccgcccaata tcagcatctg gccgccattc cagaccgcat tcgaagcgcg
      361 cgcccccggt ccgttgttgg aacaggccag cggcgtccag ttgcccgcgc tgctcgctgg
      421 agagtaagac cacagatcgc caaaataatg agtatttgcg cccactccgc catacagcaa
      481 gatttgcgaa tgcgcgctgt cccataccat cgccgcatcc gcgcgcgccg ccggccacgt
      541 gccgcccgcg catgtcgccc ccgtcacagg cgtcgcggcg gcattgatga tctgcgacca
      601 caggcccgtt acgggactat acgaccacag atcgttcaaa aagttgccct ggctatcgat
      661 gccaccgaac acgaagaata gatcatcctg ggaatcccat accatgctgt ggcgcgaccg
      721 cagcgtcgat ggagcgttgc tcgatggcgg tgccagcgcg gagagtacga cagacggaaa
      781 ggttggcgtc gccgtcgtgg taacggtcac ggatggcgta ccttgctgcg cggacggcct
      841 gacttccagc gtaaaccgaa agtccgacag gataccaggg tcagcctgcc cgacgctgcc
      901 caggatagaa aagtcggcgg tgtaggtatc aggcggcaaa ttctgagcgt tggctgtcac
      961 cgtcaatgtc tccgtgccgc ctgccgccag cgtatcggtc gaaggcgtca ccagcaacca
     1021 gttgttgata tccttatatt ctatgtacca gttgaagctc tggtcgcttt tgttcgtaat
     1081 cgtaatcgtc ccggctgccg ggtgaacacc ctcctggtca tagataaatt gcgtattacc
     1141 ggcttttgcc gagatctgtc ccaaacccgt ctgcaccgtc aatttcacgg ttaacggcag
     1201 tgcattcacg ccctgcccgt taatctccaa ctgcgcaata tcgacgccgt tggacaggcc
     1261 gtcgacattg gtcttgatcg tcaatgcagc cacctggccg ggtacgagca atccacctgt
     1321 cggcgagtag gccagccagg agtcgttgac tggattcgtc acgctccatt gcaagtccgc
     1381 cgtgccgcta tcggtgattt tcacgacctg cgtatcagca ttggtgatat ccacttcagg
     1441 cgtactgagt cgcatcgcgg ggcagacgcc cagcaggcac ggcgtaaaaa cacgcaacgc
     1501 ccccaatgtt ccaccgctga gcagcaccgc caccagcgcg agtaccgcga acacgcgccc
     1561 gtgctttctt ctggtcgggg aggatggcag tttgtcgctc tgcacctcgt agggagccgc
     1621 tctactcaac ggcgtcgttt gtttgaggac gcgtgtctca agcagttcgg tgacagggcc
     1681 ggtcgcagga tcagacttcc cgcctcgctg cttaacgatc tctccaacgg caggcagttc
     1741 agggcgcgtc gcctccttcg ccggtgtacg agcctgcacg tcggtcgcgg aattggcagc
     1801 ttcttcaact gcggcgcgat aggcgcgggc aaaggcgcgc atatctttgt agcgttccga
     1861 tggttctttt gccagcccgc gcaggatcac cgtctccacc tgctccggta cgagctggtt
     1921 cagctcgcgc agtggcggtg gcggaagttg caggtgccca tgcataatcg agtacaggct
     1981 gccggtaaaa ggagggcggc ctgccagcag ctggtaacag gtgaccgcca gtgcgtactg
     2041 atcgctggcc gggcgcggct gttccagcca ctgctccggc gcggtataaa cgggtgtgcc
     2101 aacaaagcaa tgcagtgagg catgggaata accatccagg atagtcgaga cgccaaaatc
     2161 agccagcagc agccggtcct caccatcaag cagcaggttc gccggcttga catccaggtg
     2221 gatcagcgcg tgattgtgcg cgtactgcaa ggccgccgcc gcgtcttcga catatggcag
     2281 caactgctgc ggcgtcagcg tcggcgtcag cccggcggac acacgcggca gagcatctga
     2341 cgatgcagcg agcctctcat cctgtccctc atttaccggc atatcaaccg tttcggccgg
     2401 atcaaatgac ccatcgccgt cacgcacccc actatcgccc tcctcacttg cgcgcccttc
     2461 gttcccttcc ttaccatcat cgcctccatc agccttatca gccccaccga cctgctcgtc
     2521 aatcaccacc cgggtctcga tttcttcatt gataggccgt gccgagggca gaatagacag
     2581 tccttgcggg gtaagctggc aatgttgctg gagcgagcca tgcgcggcat acttcatcac
     2641 caggtaagcg cgcccatctt ccagggttcc ggcgcgcagc acaggcaaaa tattgggatg
     2701 atcgagcgcg gcaatcgccc caacttcgcg ctcgaacagg accgccgatt cctcatcggc
     2761 cacgcgttcc ggcagcatca ccttgatcgc cacgcgccgg ctcaaaggcg gcatctgggt
     2821 cgcctcgtag acatcgccaa aagcccccga accaatccag cgcgtgacac gacaatcgcc
     2881 cagcacagcg ccttccagta aatacctgca atactggcag gccagctgct cactcacact
     2941 cacctgcgcc cctcccatag acgcgccatg cgcagacact atatctctat tctcgtgtga
     3001 tttcttcggg ttgagacaaa ataccataac tcacaccccg gcgttaaaca aaaaactccc
     3061 caatgatcgt gccatgacac caatgcagca agtgagaaaa gtatacactc attactacgc
     3121 gctaagcagc agtttttcta acaagggcaa ccacaatggt acgcccttct agacttcgcc
     3181 gctctgttcg ttgtggtcgc ccttgccaac ttcatcgccc gtatcgttcg cgccagcaac
     3241 gcgcgcgccc gcgcgaatag cctgcgcccc cgcgcggtct ttctgcgcgt ccagcgtaat
     3301 cataggaaaa gagcggtgaa actgggtttg caattgcgct gccttttcgt cagaatgccg
     3361 ggcgcgctgt tcatatgccc gcgccagcaa cgcgcgtttc tcttcgtcgg gtatatcaat
     3421 caacagcaga aaggccagcg cgaaatcagg tttgccctgc tcggcatagg tgcgcgccga
     3481 agccagccca tccataccct gttccatctg ctgacgagca tagtgtgaga atacatcttt
     3541 gagtaatgac gcataaagct catgaaacgt ctcagacacc gtatataccc tttaattatc
     3601 acacaacccg taggtcatca cacaaccctg tcggtaaata tcgtaacaca tctggcaagc
     3661 aaaagtacta ctcgctcgtt tgatacacat caatataccg accaaatgat atgatgcgcg
     3721 ggagattatc atgctgaggt gcttctatgc gtaggaacta ttggcctatc tttttcctgc
     3781 tatgcttgct gaccggctgc caatcagctt cacaggccag tcagtcgagc caactcgcgc
     3841 caacgaggcc atccgcatca tcgctcttta gcacgcccat cagcatctcc ggtcagccaa
     3901 ctccggcaaa agtatcgccc accgctcggt cagctagcca gaccgccaca aaaagcgcga
     3961 ccggcacacc cgctctcacc ccaacgtcgc aagttcaaca actggcgcag tacgttttcg
     4021 ggcttatcaa ccagagccgc gccgctcaag ggctgcgtcc ctacgcatgg ggcggcgcgc
     4081 tggcaggagg cgcgcgcctg cacaaccagc gcatgatcgc ctatggccaa ctctcccacc
     4141 agtgtccggg agagcctgcg cttggcgctc gcatcagcgc cgacggcatt caatggcagg
     4201 cggcaggcga gaatatcggc tgggcctcct acacgaacgc gcagcaaggc ctgctctcca
     4261 ttcaccagag catgatggcc gaacagcccc ccaacgacgg ccaccgccag aacattctca
     4321 acacaaacta caacctgctc ggagtcgatg tcgtgatcga ctccaaaggc caggtctggc
     4381 tgacagaaga tttcgccgaa gcgtaatgaa gctacgtccc gctgccgtac aaactgatcg
     4441 tcaccgttgg tgcgtggatg cgccccagca ggtgataatg gctgtcaagc caatcctgcg
     4501 cagactgcgc ccgcgcagcc gacgtattat cggggaagcg accgaggatg tagaagaagc
     4561 gagggtgctg cgcggcatag gcagccagcg cctggggatc gacggcggcg aaggtcccgt
     4621 ttgacgggtt ggccgggcca aattgctccc aggagaatgc gccgggtgca tccggtgtga
     4681 agtgcgccgc cgagggataa gcgtccaggt agtactgcac ggcgatctgg cagccctgct
     4741 caacatcgtt atcgaaacag accagcccat cgccgctctg gtaatgctgc tccaaccagg
     4801 gaaccgccga gttccaatct tccacctgcg cgctgcggta gtaaatcggt acgtaataca
     4861 ccgcaatcag caatacccct gcaatcaatc caacctgcac aatacgccag cgcagcgcgg
     4921 caattgccat gcccgacagc aagaagagcg gcggcagaat cgtcacgagg taacgcgacg
     4981 agaagagccg cagcgacccc tgcgaaacaa cgtagctgat gatgaccggc acgaacagcc
     5041 agcagacgag cgcgaaggca accgggaaat actgcttcaa acgggactcg tcctgctcat
     5101 tttcgccgcc agcgaaggtg acaagctgca agaccgactt cccttccggc aaaatagtca
     5161 gcagcgcgat gccgacggcg cagaagcagc acaggaagag cagcatcaag aagagtgggc
     5221 tgttggtcgt gatgtactgg aacaggtgca gaatatcatt cggctgtgga atgggcagcc
     5281 agttggtttt cgccccatgc aggctgacga gccccatagg aataccgagt acgccgatgg
     5341 ccgccagcgc cgcgccgaat cctcgcaggc gttggcgcgc ccgttctcgc cacggacccg
     5401 gcagtaccag caggccgcca aaggccacga cctgcgccat caggatcaac atagaaaaga
     5461 gctgcgcata gatggcgagt gtcgtggcca gaacaaaaca gatccaccag cgtttgcctt
     5521 tccccgtcgt cgtcagccag gcaaacagcg cgtaccacgc caggcaaatc agcagcaatt
     5581 gcaaactata cgagcgcgcc tgctgagcat aaatcagttg caggtagttg agcataaaca
     5641 aacccgcgcc ggcaatgccc gccgccatgc ccacgaagca ccgtcccagc aggaagacca
     5701 tcaccgcagc gagcgcggca aagatggccg atggcagccg caccacaaac tcggtcggca
     5761 gccagccgaa caggcccgtc agcccaaccc agccgtgcag gaacaggtag taaagttcca
     5821 tattcggctc tggcccccag ataatatgcc acaacaacgg cagtggctgt ttcgccagct
     5881 caaccgaaaa ggcttcatca aaccagacgc tcggattccc cagccggtac agatcaaacc
     5941 ccagcgcgcc cccaaagacg atgagacctg ctatcaacgt atgagcgcgc ggattccgca
     6001 aaaaaacggg tacgccgcgc ccggtgcgag ttcgccccgc tcttcgcgtc ccttctggcg
     6061 atgtggttcg ttgtgcgtct tgcgcttcgt ctacccggct catgcctgtc cgatctcctt
     6121 ctctctatta ggagaacgtt ccccgcaagc gtcttgtaac agacatagga cagtgcgtgt
     6181 attgtatgag gtgtcggaaa gcagccggtt atgtaggttt ataaccagga tcgggaatgt
     6241 catcctgagc gcagcgaagg atctgcgggc catg
//
LOCUS       DASZAK010000273         4029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_539477, whole genome shotgun sequence.
ACCESSION   DASZAK010000273 DASZAK010000000
VERSION     DASZAK010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539477"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="VGT44_04715"
     CDS             complement(<1..1775)
                     /locus_tag="VGT44_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEV2580136.1"
                     /translation="MPSQGYVRYPTIHHDQIVFVSEDDLWLVSSEGGRASRLTAGVGE
                     VSLPRFSPDGSLLAFVGREEGPGEVYVMPATGGVAQRLTYQGASCRVAGWTPGGESIL
                     YASNAGQFTHRFEILYTISPNGGEPQKLSFGLANAISHGPHGGVVIGRNMNVRDFSYQ
                     KRYRGGRVGHLWLDNDGSGNFQRLLRLDGNIADPCWVGDRIYFLSDHEGVGNIYSCTP
                     RGDDVRRHSYHNDFYARHLSSDGQRLVYHAGADLYLFDPAEEISHHLEVELPSIRTQR
                     QRKFSYAGSYLDGYRLHPQGYAVALTTRGKAFTLGNWEGPVLQHGELDGVRYRKLSWL
                     NDGKRLVAVHDATGRESLVVFNPEDGSEAQTLPDIDFGRVSGLDVSPTEDMLALTNHR
                     NELVLIDLEKSEARVLDRSEYGRIEGFDWSPDGRWIAYGFPFTNQRTAIKLCNIETSE
                     THFVTDPILEDFDPVFDPEGKYLYFLGHRILNPVRDSLQFDLSFPRGTKPYVITLQRD
                     LRSPFMPEPKAPGEKDKEKNNGKKEKEKEEPDKDEDAEVGEESEAGDKEQEDKTEKNN
                     DDKRPPAIVIDLEGIASRVLPFPVA"
     gene            complement(1929..2525)
                     /locus_tag="VGT44_04720"
     CDS             complement(1929..2525)
                     /locus_tag="VGT44_04720"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2580137.1"
                     /translation="MTTKRREKDIERFDNWANTYEHSWLQRAFFDRTHQAALALAAGI
                     VHQPASVLDVGCGTGKLLRRATTYWPEAQLIGVDPANGMIELAKRLTPNATFFTGMAE
                     ELPLEDASVDLALSTSSFHHWQDQAAGIREIVRVLRPGGYFLLVDASLPDWLAPVFRP
                     KRFHSPAKLRTLFIQAGLHVQSQQSLAWRRGLATVGNK"
     gene            complement(2842..>4029)
                     /locus_tag="VGT44_04725"
     CDS             complement(2842..>4029)
                     /locus_tag="VGT44_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580138.1"
                     /translation="CTLGNVGSDLSVTGMMKVHVGSVGGDCNVRDAQGGLEIGNVGGD
                     ASLHNIGESMNVGNIGGDASMKDIQSAIEAGNIGGDLLLDATFPPGSATRLHVGGDAV
                     LRLPTPSDLTLRAFVGGDIGGISTASGKDNQVNVVYGSGAARIELYVGGDLAVRGGGQ
                     PTSSSSYGGHRNAGNRPGWQAEFEHEMAELGREMGKLGQEISREITGAFKDAGWQKGS
                     EWANSFTDKMEEQIRRAQRKADEQARKANEQARKASEQAARVRVRFNDREWQVDPARL
                     ERLKEQASRAANEGIAGALDAVERAIQNLRVPPPPRPPTPPRPPVVPVPPVSPVDPVA
                     PVPPMPGQPPMTQSAPPDAPEDSGTSAEPNIEQEREAILRMIAEGRISPEEGDMLLEG
                     LGG"
BASE COUNT          795 a         1210 c         1153 g          871 t
ORIGIN      
        1 tcggcaacag gaaacggcag gacgcggctg gcgatgcctt ccaggtcgat cacgatggcg
       61 ggcggcctct tatcgtcgtt gttcttttcg gtcttgtctt cctgctcttt gtcgccagcc
      121 tcgctttctt ctccaacctc agcgtcttca tccttgtcag gctcctcttt ctccttctcc
      181 ttcttgccgt tattcttctc cttgtccttc tcgccgggcg ctttcggttc cggcatgaag
      241 ggagagcgca gatcgcgctg caaggtgata acgtaaggtt tcgtgccgcg tgggaaactc
      301 aaatcgaatt gcaggctgtc gcgcaccgga ttgaggatgc ggtgtcccag gaagtacagg
      361 tatttccctt cgggatcgaa gaccgggtca aaatcttcca gaatggggtc cgttacaaaa
      421 tgcgtttcgc tcgtctcgat attgcagagc ttgatggcgg ttctctggtt ggtgaaggga
      481 aagccatagg cgatccagcg gccatcgggc gaccagtcga agccttcgat gcggccatac
      541 tcgctgcggt cgagaacgcg cgcctcgctc ttttcgagat cgatcagcac cagctcgttg
      601 cggtgattgg tgagagcaag catgtcctcg gtcggcgaaa catccagccc cgaaacgcgg
      661 ccaaagtcga tatcgggtag ggtctgggcc tcgctgccat cctctggatt aaagacgacc
      721 aggctttcgc gcccggtcgc atcatgcacg gccaccagcc gtttgccatc attcagccag
      781 ctcagtttgc gataacgcac accatccagt tcgccatgct ggagaaccgg tccttcccag
      841 ttgcccaacg taaacgcttt gccgcgcgtg gttagcgcaa cggcgtatcc ctgcgggtgc
      901 agccgatatc catcgaggta cgaaccggca taagagaatt tgcgctggcg ctgcgtgcga
      961 atgctgggca gttcgacctc caggtgatgg ctgatttctt cggcggggtc gaacaggtat
     1021 aggtccgcgc cggcatggta gaccagccgc tgtccatcgc tcgacaggtg gcgggcgtag
     1081 aaatcattat gataggaatg gcggcgcacg tcatcgcccc gcggtgtgca ggaatagata
     1141 ttgccgacac cctcgtgatc agaaagaaag tagatacggt cgcccaccca gcacggatcg
     1201 gcgatgttgc catctagccg cagcaggcgt tggaagttgc cgctgccatc gttatccagc
     1261 cagagatgcc cgactctgcc gccgcgatag cgtttctgat aagagaagtc gcgcacattc
     1321 atgttgcgcc caatcaccac accgccatgc ggcccatgcg aaatggcgtt ggccaggcca
     1381 aaggagagct tctgcggttc gccgccattg ggagaaatgg tatagagtat ctcgaagcgg
     1441 tgcgtgaatt gaccggcatt gctggcatat aaaatcgact cgccgccggg cgtccagccc
     1501 gcaacgcgac aggacgcgcc ctggtaggtc aggcgctgcg cgacgccccc ggtggctggc
     1561 atgacataca cctcgcctgg tccttcctcg cgccccacaa aggccagcag cgagccatcc
     1621 ggcgaaaagc gcggcaggct gacctcgcca acccccgccg tcaggcgcga agcccgcccg
     1681 ccctcgctcg aaacaagcca cagatcatcc tcggaaacaa acacaatttg atcatgatga
     1741 atagtcggat agcgaacata cccctgggac ggcataagaa ctccttcata gtcgccttga
     1801 tgatgacacg acgcgcaggc acaattcaat agttaataga tatgctaaac cagactagag
     1861 ttttgggggt tagcgtattg ctgtattgta tcatataatg ctggggtaag ggcaaagcaa
     1921 aagcttcctc atttatttcc aacagtggca agccctctgc gccaggcgag tgattgctgc
     1981 gactggacat gtaatcccgc ctggatgaag agcgttcgca atttagcggg gctatgaaag
     2041 cgcttcggac gaaaaacggg cgccaaccag tctgggaggg aggcatccac aaggagaaag
     2101 tagcccccag ggcggagtac gcgtacgatc tcgcgtatgc ctgctgcctg gtcctgccaa
     2161 tggtggaaag agctggtgct cagtgccagg tcaaccgagg cgtcctctag tggcaattct
     2221 tctgccatgc cggtaaagaa cgtggcgttg ggagttaagc gttttgccag ctcgatcatg
     2281 ccatttgccg ggtctacgcc gatcaattgc gcctccggcc aataggtagt ggcccgacgc
     2341 aacagtttac ctgttccaca gccgacatca aggacgctcg caggctgatg cacgatccct
     2401 gctgccaggg cgagcgctgc ttgatgggtc cgatcaaaaa aggctcgttg cagccaggaa
     2461 tgttcatagg tgtttgccca gttatcgaag cgctctatgt ctttttcgcg ccgttttgtt
     2521 gtcatctttg tttctccttt cccaaatatc tacgttgtgt gccatcctcc tttccttttg
     2581 aaccataaag gtatgccgga gggcaggaaa catagcaaat cgcatgacat ttctagcgaa
     2641 cagggccgga ccagcgatca gggcgcaatg aagaagtttc acgaccacca tcggtcctgt
     2701 catcctgagc gcagcgaagg atctcacacg ccgaccaccg agatccttcg ctgcgctcag
     2761 gatgacacat tcataccgat cttgtttgtg aaacttcatc aatgcgccct tacacgccaa
     2821 ttgaagaatg acagtccagt attatcctcc caatccttcg agcagcatat cgccctcttc
     2881 gggggagata cggccctcgg cgatcatgcg caggatagcc tcgcgttcct gctcgatgtt
     2941 gggttcagca gaggtgccgc tatcctccgg cgcgtcggga ggcgcgctct gcgtcatcgg
     3001 cggctgccca ggcatcggcg gcaccggcgc tactggatca actggggata ccggcggcac
     3061 tggcacgacc ggggggcggg gtggtgttgg tgggcgcggc ggaggcggga cgcgcaggtt
     3121 ctggatggcg cgttcgacgg catccagcgc gccagcaatg ccctcgttgg ccgcgcgcga
     3181 tgcctgctcc ttgagccgtt ccaggcgcgc cgggtcgact tgccattcgc ggtcgttgaa
     3241 gcgcacgcgc acgcgagcgg cctgttcaga agctttacgc gcctgctcat ttgccttgcg
     3301 cgcctgctcg tcggctttgc gctgggcgcg gcgaatctgc tcctccatct tatcggtgaa
     3361 gctattggcc cattccgaac ctttctgcca gccggcatcc ttgaaagcgc cggtgatttc
     3421 gcggctgatc tcctggccaa gcttgcccat ctcgcgacct agctccgcca tttcgtgttc
     3481 aaattcagcc tgccagcctg gccggttgcc cgcattcctg tgaccgccgt agctgctgct
     3541 gctcgtcggc tgtccgccgc cgcgcacagc caggtcgcca ccaacataaa gttctatgcg
     3601 cgctgcgccg ctgccgtaga ccacatttac ctggttgtct ttgccagatg cggtcgatat
     3661 accgccaatg tcgccaccga cgaaggctct gagtgtcaga tcagatggcg tagggaggcg
     3721 caggacagca tcgccgccaa cgtgcaaacg ggttgcgctt cctggaggaa aagtagcgtc
     3781 cagaagcagg tcgcctccga tgttgccagc ctcgatcgcg ctctggatgt ccttcatact
     3841 ggcgtcgccg ccgatattgc cgacattcat agactccccg atattgtgaa gactggcatc
     3901 gccgcccacg ttgccgattt cgaggccgcc ctgcgcatcg cgcacattac aatcgccgcc
     3961 gacgctgccc acgtgtacct tcatcatacc cgtcacggaa agatcgctgc cgacgttgcc
     4021 cagcgtgca
//
LOCUS       DASZAK010000274         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_539504, whole genome shotgun sequence.
ACCESSION   DASZAK010000274 DASZAK010000000
VERSION     DASZAK010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539504"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="VGT44_04730"
     CDS             complement(<1..1393)
                     /locus_tag="VGT44_04730"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEV2580139.1"
                     /translation="MTNDMRFKILVVATEAVPFVKEGGVADVIGSLPKELAELGHEVR
                     IFLPRYGSIDPMRWGLTPTGVVKVLPMAGLDRTMSILQTTLPDSPVTVYLLDNWEFFG
                     RHQQIYLGLDQRDEQRRFLLFCRGLLEALPQLGFAPDIIHLNDWQTAPCAAYLRTTYR
                     HLLRHGAARIVYTVHNLQYQGRWDPSILDEAGLDRARVFTGSGLEFWGDVNWMKAGIV
                     YADIVTTVSRRYAEEIQTLDYGWGLDEVLFQRHPRVFGIPNGLDWDAWNPSTDSDLVA
                     RYTATEAPAAKVRNRTALRREFGLPDDPELPVVGIVSRLVDQKGFDLIAAIASELRNL
                     PLNLVVLGSGQPRYEQLFRELATSTANVRAHIGFSVALSRRIYAGADFFLMPSAFEPG
                     GLGQLIALRYGALPIVHATGGLADTVIDLDAYPDKGNGVSFTPYNASALLEALHRALR
                     LYAQHDRWPGLIER"
     gene            complement(1460..>2068)
                     /locus_tag="VGT44_04735"
     CDS             complement(1460..>2068)
                     /locus_tag="VGT44_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEV2580140.1"
                     /translation="LQVRLSYIGLEVPPEVIFTSALATAQFLNDQHPGGRAYVIGESG
                     LTTALHDIGYVLTDQEPEFVVLGETTAYSFDRITRAIRFVSAGARFIATNPDVMGPGE
                     GGIVPATGAVAALISAATGVKPYYIGKPNPLMMRTALRTLNAHSEDSVMIGDRMDTDI
                     VAGIESGLRTLLVLTGVTTHEQVKRFPYRPTWIRESIAEVEV"
BASE COUNT          446 a          630 c          614 g          378 t
ORIGIN      
        1 ctcgctcgat gagaccgggc cagcggtcgt gctgtgcata gagccgtaat gcacgatgca
       61 gcgcttcaag cagggcggag gcgttgtacg gtgtaaagct caccccattg cctttgtcgg
      121 ggtaagcatc cagatcgatc accgtgtcgg ctaaaccgcc agttgcatgg acaattggca
      181 gcgcaccata gcgcagcgcg atcaactgcc ccaggccacc cggttcgaag gcgctgggca
      241 tcaggaagaa atccgcgccg gcgtagatgc ggcgagagag ggcaacgctg aagccgatat
      301 gcgcacgaac gttagcggtg ctcgtggcaa gctcacggaa taattgctcg tagcgaggct
      361 gcccgctgcc caacaccacc agattcagtg gcaggttgcg caactcagag gcgatcgcgg
      421 cgatgagatc gaaacctttc tgatctacaa gccggctgac gatgcccacc actggcagct
      481 ctggatcgtc cggcagcccg aactctcgcc gcagcgcagt gcgattgcgt actttggcag
      541 ccggtgcctc cgtcgcggtg taccgggcaa ccaggtcact atcggtcgag ggattccagg
      601 cgtcccaatc gaggccattg ggaataccga agacgcgcgg atggcgctga aagaggacct
      661 catcgaggcc ccagccatag tccagcgtct ggatctcctc ggcgtagcgc cgcgagaccg
      721 tcgtcactat atcggcatac acgatgccgg ctttcatcca attcacgtcg ccccagaact
      781 ccagaccgct ccctgtgaag acgcgcgcac ggtccagccc cgcctcatcc aggatggagg
      841 ggtcccagcg cccctggtac tgcagattat gcacggtata gacgatacga gccgccccgt
      901 gacgcaggag atgacgatag gtggtgcgca ggtaagcggc gcacggggca gtctgccaat
      961 cgttgaggtg gatgatatcg ggggcaaagc cgagctgagg cagagcctcg agcaaacccc
     1021 ggcaaaatag cagaaagcgt cgctgctcat cgcgctgatc cagaccgagg tagatctgct
     1081 ggtgccggcc aaagaactcc caattatcaa gcaggtagac ggtgaccggt gaatcgggaa
     1141 gcgttgtctg caagatagac atagttcggt caagcccggc catgggcagc actttcacga
     1201 cacccgtagg tgtgagaccc cagcgcatgg gatcaatact cccatagcgg ggcagaaaaa
     1261 tgcgcacctc gtggcccaac tccgccagtt ctttgggcag gctccctata acgtcggcca
     1321 caccaccctc ttttacaaag ggaaccgcct ccgtagcgac caccaggatc ttgaagcgca
     1381 tatcgtttgt catgctagcc tcattttgtt gagcattcct cagacgggaa cggcacgtct
     1441 gctcttgtaa gaagatacct tacacctcga cctccgcaat agactcccgt atccaggtag
     1501 gtcgataggg aaagcgtttc acctgttcgt gcgtggtgac tccggtgagc accaggagag
     1561 tgcgcaggcc gctctcgatc ccggcgacga tatcggtatc catgcgatcc ccgatcatca
     1621 cagagtcttc agaatgcgca ttcagggtgc gtagcgccgt gcgcatcatc agcggattcg
     1681 gtttgccgat gtagtaaggc ttgacgcccg tggccgcgct gatcaacgcc gcaactgcgc
     1741 cggtcgcggg aacgattccc ccctcgcccg gccccatcac gtccggattg gtggcgatga
     1801 aacgtgcccc ggcactcacg aagcggatgg cacgggtaat gcgatcgaag ctgtaggcgg
     1861 tcgtctcacc cagtacgaca aactccggct cttgatcggt caggacgtag ccgatgtcgt
     1921 gcagcgccgt cgtcaacccg gattcaccaa tgacgtacgc tcgcccgccc ggatgctgat
     1981 cgttcaggaa ctgtgcagta gccagcgcgg atgtgaagat cacttcgggc ggcacctcca
     2041 gccctatata tgacaggcgt acctgcag
//
LOCUS       DASZAK010000275         4892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_220019, whole genome shotgun sequence.
ACCESSION   DASZAK010000275 DASZAK010000000
VERSION     DASZAK010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220019"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..701
                     /locus_tag="VGT44_04740"
     CDS             135..701
                     /locus_tag="VGT44_04740"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV2580141.1"
                     /translation="MSKENKSKYAVLGVLSIYPGSGYDIKKFMEQSTANFWQESYGQI
                     YPILKQLADEGLATSHTEKQEGKPERYVYTLTERGGLALQEWLSESVEQSIERNELLL
                     KLFFGGHISHEKNMEHVQSFRALQSQLLVKYEGIERSLRAGAKEDHNLNYPLMTVRYG
                     IHRCRALVAWCDETLQSLEAMRSERDAG"
     gene            732..1244
                     /locus_tag="VGT44_04745"
     CDS             732..1244
                     /locus_tag="VGT44_04745"
                     /inference="COORDINATES: protein motif:HMM:NF025196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HEV2580142.1"
                     /translation="MAKVMPGRFTAQVDGPFVVFMIGMRVNKFFALRKWIPTATAMGP
                     MLRTLYQHPEKGFLGAQTFFNLRGVVTMQYWRSFDDLEKFARDKNDPHLESWRRFNKT
                     IGSDGSVGIFHETYLVDAGKYEALYGNMPVFGLASATQHIPAVGKRETARRRLGGEGE
                     PAVPSPAQSL"
     gene            1339..1683
                     /locus_tag="VGT44_04750"
     CDS             1339..1683
                     /locus_tag="VGT44_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580143.1"
                     /translation="MSQQMHYDEAERERQGAPIDHYDAGYQGGYRDPYAGGQKISFGN
                     TSFQDVKDKTASAGQRLALAIVSVAMLVPLAGIIMGTTFTQFFSLVGGLIAMAIVCVT
                     IMVINIVFNFRH"
     gene            complement(1667..2602)
                     /locus_tag="VGT44_04755"
     CDS             complement(1667..2602)
                     /locus_tag="VGT44_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV2580144.1"
                     /translation="MQQTAIIIANPNSGYLPHHPNRLQEALTYLRRHGWQADLWFTVQ
                     RGDGQRLAQKAVEQGTTVVVAAGGDGTINEIIQGLAGSETALGVLPTGTVNVWAREVG
                     IPLDVHEACNVLVNGQTRHIDLGCVNGQYFLLMVGIGFDAEMTQKVERKPLKRLGPLG
                     YALAALWFGPGYVGFPVIVRRDDHAIKTRALQVFIGNTQLYAGAFKFTWKARCDDGQL
                     DLCIVRKRNLLGRLVVLWDFVLRRDSRRRWVRYNTFTSITLETPHPVAFQIDGDPGGY
                     TPATLTVRPRALKVVVPQKTPAGLFSDESLSNAGS"
     gene            complement(2605..>4892)
                     /gene="alr"
                     /locus_tag="VGT44_04760"
     CDS             complement(2605..>4892)
                     /gene="alr"
                     /locus_tag="VGT44_04760"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV2580145.1"
                     /translation="ILRRWHPAVIAVSGSVGKTSTKEAIAAVLGSSFATFKSWQNYND
                     LLGLPLSLGRLEERHEFAVLELGCDHPGEMQQLCEMVNPTIGVLTNASPTLLQYFGTY
                     DRLVEEYGKLLTFLPESGFVLLNASDEPSLALLASVSKSIKAPVLQFHPSLALNADIT
                     WEGIAFDIPPTKHLTSHLLGRHHIATMLAAYTIGQRCGVPPAERQHALASLTPLPGRL
                     QPLPGVRGSRLLDDTHNATPLSMLAGLETLKALPAGRRIAVLGDMSRLGEIEKEAHRA
                     VGQQTARCADYLVLRGEYATLVAEAARKSGMMDDRIIITSTHEDAAQAAERLVEESEL
                     YVGQDASNRVPTIVVKGSEEARMERVTERLMAQPEQARERLVRQTPGWKQIVVSRPSR
                     PTWVEIDLSAIANNTRRIAEIIGPQVRILASLKADAYGHGALKVAHTVLHNGATMLGV
                     ATVSEAMPLRQAGMRAPILVFGYTPLWQMREAVHLDLTITLYTLEAAQALSRAAESLG
                     KSVKAHIKVDSGMGRLGIRAEEVDEVVQLVQSVIDLQGLELEGIFTHLATADAQDQHH
                     TRLQLARFRRVLQALEEKGLRPPLVHAANSAALLSLPESRFDMVRPGIALYGLDPSEV
                     VRLPQSFHPALSFKTQVSLVKQVPAGECISYGCAYTTERPMTVATLPVGYADGFRRGP
                     ANWGSVLIHGQEAPLLGRVCMDQCMVDVTHIPQVRIGDEVVLIGRQGHSSLSAEEVAR
                     RLGTIHYEVVSEILARVPRVD"
BASE COUNT         1046 a         1371 c         1466 g         1009 t
ORIGIN      
        1 tactcaccga ccggaatcaa attttcacct gacgggagca tcatcccagg tttagagagg
       61 gttcagcttt tccctcttga catctttcta tctttatggt atatatctat cagatatatt
      121 acttggagat agatatgtcg aaagaaaaca aaagcaagta tgccgtgctg ggggtactca
      181 gtatctatcc tggttctggc tatgacatca agaaattcat ggagcagagt accgcgaatt
      241 tctggcagga gagttacggg caaatttacc cgatcttgaa gcaactggcg gatgaagggt
      301 tggcgaccag ccacaccgaa aagcaggaag gcaagccgga acgctatgtc tataccctga
      361 cggaacgggg cgggctagcg ctgcaagagt ggctctcaga atcggtcgag cagtcgatag
      421 agcggaatga gttgctgctg aagttgtttt ttggtgggca tatctcacac gaaaaaaaca
      481 tggagcatgt gcaatcgttt cgcgccttgc agtcgcaatt actggtgaaa tacgagggta
      541 tcgagcgcag cctgagagca ggagctaaag aggatcacaa cctcaactat cctttgatga
      601 cggtacggta tggcattcat cgctgccgcg cgctggtcgc gtggtgcgat gaaacgctgc
      661 aatcgctaga agcaatgcga agcgagaggg atgctggata gctcgcaacc tttgcaaagg
      721 agatcaaatt catggcaaaa gtgatgcctg ggcgctttac agcgcaagtc gatgggccat
      781 ttgtagtgtt tatgattgga atgcgtgtca acaagttctt tgccttacgc aaatggattc
      841 cgacggctac ggcgatgggg ccgatgctgc gcacgctcta ccaacatcct gagaagggct
      901 ttcttggggc gcagacgttc tttaacctgc gtggagtagt gacgatgcag tactggcgct
      961 catttgatga cctggagaaa tttgccaggg ataagaacga cccgcacctg gaatcctgga
     1021 gacgctttaa taaaacgatc ggctcggatg gcagcgtggg catctttcat gagacgtacc
     1081 tggtggacgc tggcaagtat gaagcgctct acggcaatat gccagtattc ggactggcat
     1141 ctgccacgca gcatatcccg gcagtgggca aacgcgagac agcgcgtcgc cgcctgggtg
     1201 gagaaggcga gccggcagtg ccgtcccccg cgcaaagtct gtagggacag aatagcctcg
     1261 cttgcaactc gaatcacatc cgtgcgtatc tacaatatgc gcgggaaatg gcatatcata
     1321 cgaggagaag cagctattat gtcgcaacaa atgcactatg atgaggccga acgagagcgc
     1381 cagggcgcgc cgattgatca ctatgatgca gggtaccagg gtggatatcg tgatccgtat
     1441 gccgggggcc agaaaatctc gttcggcaac acgagttttc aggatgtaaa ggacaaaaca
     1501 gccagcgcgg gtcagcggct ggcgctggcg attgtatcgg ttgcgatgct ggtgccgttg
     1561 gcgggtatca tcatggggac gaccttcacg cagtttttct cgctggtggg cgggttaatc
     1621 gccatggcga tcgtgtgtgt gacgatcatg gtgatcaaca tcgtgttcaa cttccggcat
     1681 tagataggct ctcatcggag aacagacctg ccggcgtctt ctgcggaacg acgactttca
     1741 gggcgcgtgg ccgcactgtc agggtggcgg gagtatagcc gccggggtcg ccgtcgatct
     1801 ggaaggcgac gggatggggc gtttcgagcg tgatggatgt gaaggtgttg taacgcaccc
     1861 agcggcggcg gctgtcgcgt cgcaggacaa agtcccacaa gacgacaagg cgtcccagca
     1921 ggttgcgttt gcgcacgata cagagatcaa gctggccatc gtcgcagcga gccttccagg
     1981 taaatttgaa ggctccggcg tagagttgag tattgccgat gaagacttgc agcgcgcgcg
     2041 tcttgatggc gtgatcgtcg cgtcgcacga tgacgggaaa gccgacatag ccagggccaa
     2101 accagagcgc cgccagcgca tagcctaacg gccccaaccg tttcaagggc ttgcgttcca
     2161 ccttctgtgt catttcggcg tcgaagccaa tgccaaccat caacaggaaa tattgcccgt
     2221 tcacacaacc caggtcaata tggcgtgttt gcccattgac gagtacatta caggcctcgt
     2281 gtacatccag cggaatacca acctcgcgcg cccacacgtt gaccgtgccg gttggcagca
     2341 cacccagcgc cgtctcgctg cccgccagcc cctggatgat ttcgttgatc gtgccatcgc
     2401 cccctgccgc gacgacaacg gttgtaccct gctcgactgc cttttgcgcc agtcgctgcc
     2461 cgtcgccgcg ctgcacggtg aaccacaagt cggcctgcca gccatgtctg cgcaggtagg
     2521 tcagcgcctc ttgcaagcgg ttcggatggt gcggcaggta gcctgaattt ggattggcaa
     2581 taataatggc agtttgctgc atattcaatc cacgcgcggc acgcgcgcga gtatctcgga
     2641 gacgacttca taatgaatgg tacccaggcg gcgcgcaact tcctctgccg agaggctgga
     2701 gtggccctgg cgtccgatca ggaccacctc gtcaccgatg cgcacctggg gaatgtgcgt
     2761 gacatcgacc atacattgat ccatacagac gcgccccaac agaggcgctt cctggccgtg
     2821 aatcagcacc gatccccagt tggcggggcc tcgccggaac ccgtcagcat agccgacggg
     2881 cagcgtcgca accgtcattg gccgctccgt cgtataggca cagccgtagc tgatgcattc
     2941 accggcgggt acctgtttca ccagcgaaac ttgcgtcttg aacgagagcg cgggatgaaa
     3001 gctctgtggc agccgcacaa cctcggatgg gtccaggcca tagagcgcga tgcctgggcg
     3061 caccatgtca aaacgcgact caggtaggct gagcagggcc gcgctattgg ctgcatggac
     3121 caacggcggg cgcaatcctt tttcctccaa cgcctgcaag acgcgccgga aacgcgccag
     3181 ttgcaagcgg gtgtggtgct ggtcttgcgc gtctgcggta gcaaggtggg taaagatacc
     3241 ttccagctcc agaccctgta gatcgataac ggactggact aattgtacaa cttcatcgac
     3301 ctcttccgcg cgaataccca gacgtcccat gccgctatcg actttaatat gcgccttcac
     3361 cgatttgccc agcgattcgg cggcgcgcga cagcgcctgg gctgcctcca gcgtatagag
     3421 cgtgatggtc agatcaagat gcacggcttc gcgcatttgc cagagcggag tgtagccgaa
     3481 gaccaggatg ggcgcgcgca tccccgcctg gcgcaggggc atggcctcgc tcaccgttgc
     3541 cacgccgagc atagtcgcgc cattgtgcag cacggtatgg gctaccttga gcgcgccgtg
     3601 tccatatgca tcggccttga ggctggccag gattcgcacc tgcggcccga tgatctcggc
     3661 gatgcgtcgc gtgttgttgg cgatggcgct caagtcgatc tcgacccacg ttggacggct
     3721 gggacgcgaa acgacaattt gcttccagcc gggcgtctgc ctcaccagcc tttctcgcgc
     3781 ctgctccggc tgcgccatca agcgttccgt cacacgctcc atgcgcgcct cttccgaccc
     3841 cttcactact atcgtcggaa cgcggttgct cgcgtcctgg ccgacataca actcgctctc
     3901 ttcaactaac cgctcagccg cctgggctgc gtcctcgtgc gtcgaggtaa tgatgatgcg
     3961 gtcatccatc ataccggact tgcgcgcagc ctcggcgacc agagtggcat actccccgcg
     4021 cagcaccagg taatcagcgc atcgcgcagt ctgctggccc acagcgcggt gcgcctcctt
     4081 ctcgatctcg cccaatcgtg acatgtcgcc cagcacggcg atgcgacgcc cggcaggtaa
     4141 cgcctttaac gtttccagcc ctgcaagcat cgagagtggg gtggcgttgt gtgtgtcatc
     4201 caagaggcgc gaaccacgca cgccgggcag cggctgtaag cgtcctggca gaggtgtgag
     4261 cgatgccagg gcgtgctgtc tctccgcggg aggcacaccg cagcgctggc caatcgtata
     4321 ggcggccagc atggtcgcga tgtgatgtcg ccccaggaga tgtgaagtca ggtgctttgt
     4381 gggaggtata tcgaaggcga tgccttccca tgtaatatcg gcattgagcg ccaatgatgg
     4441 atgaaactgc aacacaggcg cttttatcga tttgcttaca ctggcaagca gcgcgagcga
     4501 tggttcgtca gaggcgttga ggagaacgaa gccgctttca ggaaggaagg ttagcagctt
     4561 accgtattcc tcgaccaggc gatcatacgt gccgaaatat tgcagcaatg ttgggctggc
     4621 gttggtgaga acgccaatag taggattgac catctcgcag agctgctgca tttcaccggg
     4681 gtggtcgcag cccagctcca acactgcaaa ttcgtggcgc tcttccaggc gaccaagcga
     4741 gagcggcaga cccagcaggt cattgtagtt ctgccagctt ttgaaggtgg caaagctgct
     4801 tcccaatacg gctgcaatgg cctccttcgt gctggtcttg cccacactac cgctcacggc
     4861 gatcacggcg ggatgccagc ggcgcaggat gg
//
LOCUS       DASZAK010000276         5861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_42170, whole genome shotgun sequence.
ACCESSION   DASZAK010000276 DASZAK010000000
VERSION     DASZAK010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42170"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..962
                     /gene="gluQRS"
                     /locus_tag="VGT44_04765"
     CDS             <1..962
                     /gene="gluQRS"
                     /locus_tag="VGT44_04765"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEV2580146.1"
                     /translation="YAPSPTGELHLGNLRTALLAWLLARCAGGQFVLRVEDLDHPRVR
                     PGTTEGMLRDLRWLGLDWDEGPDRGGPYAPYTQSERQTIYAQHLRRLQEAGLLYPCYC
                     SRADLARAASAPQGDEGPRYPGTCRNLTEAQRRQREANGRRPSLRFRVAEERVVTFDD
                     MLLGRQEQHTQRAVGDFIALRSDGIFSYQFAVVVDDALMRINQVARGADLLSSTARQI
                     LLYEALGFSIPTFAHVPLMLDANGQRLAKRQQSAGLEPLRAAGVSPASVVGQLAASCG
                     LIAPGTQISANELAEQYSGAGYDMMRTAFLPPIYRSKARFKRS"
     gene            1143..1409
                     /locus_tag="VGT44_04770"
     CDS             1143..1409
                     /locus_tag="VGT44_04770"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEV2580147.1"
                     /translation="MAKKCTHLDQIQEVTPSANGCEDCLKIGDTWVHLRLCLICGHVG
                     CCDSSKNKHATRHFHATNHPIVQSFEPGEDWVWCYIDQVMLEPA"
     gene            1505..2434
                     /locus_tag="VGT44_04775"
     CDS             1505..2434
                     /locus_tag="VGT44_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEV2580148.1"
                     /translation="MENISNTQQKERGEGWPARPYEAWRDTCQTLHLWTQIVGKVRME
                     LSPFLNHWWHVTFYVTPRGLTTSSIPYQGGVFEVIFDFVEHNLCIRTSEGTSRALPLI
                     PRSVAAFYREFMACLHALGIEVTINPLPSEVQNPIRCDVDEVHASYDPVYAQRFWRIV
                     AQTDAVLQRYRSPFLGKSSPVHFFWGSFDLALTFFSGRRAPERPGADRMTREAYSHEV
                     ISCGFWPGDDRFPTPAFYSYTSPEPPGLPAASIRPAEAIYNHELGEFFLRYDDVRTAS
                     SPELALLEFFQSAYEAGARLAQWDREALERKAI"
     gene            2740..3816
                     /locus_tag="VGT44_04780"
     CDS             2740..3816
                     /locus_tag="VGT44_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEV2580149.1"
                     /translation="MAGIVLSVVLTILVVAVALGVAFWLRRLIVRRLKKTVLDNWLVQ
                     TIGALVTIPVIILGLLLVSLTVTMSVNFIITLWSGITAGLQEQTIANGIRALLWDFIV
                     TILIIILAIGVGRTVMRLIVGRLGEQRLDINIRTLIGRIFYGLIVMVSVFWILSLWQV
                     AIDVPIAVISALSVAFTFSIQDILKDLVAGLYILFERPFHIGEQITTATYTGTVENVE
                     LRATKIRLTSGEEITIPNALVFGGIVVNNSQFAERRASITLTMPQEEFDKDETPPTIL
                     DVVRKQENVLVKPEPHVFVNHYAGTFGGTTGVKTGYSGQIVVLTLRFWMPSGRYDTVT
                     EVMSALHEALPTVDLLVEDSGGNV"
     gene            3843..4538
                     /gene="nadD"
                     /locus_tag="VGT44_04785"
     CDS             3843..4538
                     /gene="nadD"
                     /locus_tag="VGT44_04785"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV2580150.1"
                     /translation="MQYNKSFPVTRRIGVIGGTFDPVHYGHLVIAEEVYAALDLEEMV
                     FVPTGQPPHKPDRVVTPAEHRLAMLERAIAGNPHFSLSRVDVDRAGPSYTVETLRLLR
                     QQWEAQAQQADDVERIELSGSIALYFVIGWDSLEYLLTWHDPAGILEQLDYLVAVKRP
                     RYNEDGTIYLDALETRLPGLKQRLLVVSAPQLDISSTDLRARVAQGRPIKYQLPESVE
                     EYIREYGLYSHAG"
     gene            4528..4740
                     /locus_tag="VGT44_04790"
     CDS             4528..4740
                     /locus_tag="VGT44_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEV2580151.1"
                     /translation="MPDNQSPMHIDLGDRLRLRKPHPCGGYDWLVVRLGADIGLRCEK
                     CGRRVLLPRAEVERRTKQVLTRAMDA"
     gene            4764..>5861
                     /locus_tag="VGT44_04795"
     CDS             4764..>5861
                     /locus_tag="VGT44_04795"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580152.1"
                     /translation="MALANPQGGFLTLLKKRNFLLLWLAQLISMTVLNASNYALIVLI
                     EVITGSTTLIGLAIVCFSLPAVLFGAPAGVFVDHRNKRRVLWYSNCLRAIATFGFVLS
                     LLLTSHLSTSLQLIPVYLLTFLISGIGQFFSPAEGASIPLLVSEEELMPALSLFNITF
                     MLAQAFGFIIFAPILLSVLPTFTLFSVSVGAVPTLYAIFALLYLVCAVLVSLIPAQSF
                     EENGHAPRDRETIGTQSLGAFSNVMSEMRQGWQFIRRQPPLFLPVIQLSFAGILLLVI
                     GELATPLVTRLFLLPASSMAVIFAPAGLGLVIGSIFMPRVTRRIGKSRTIFIGSVSLA
                     STIVLLPLLSWLTTTLIAHGINFRPAQFAIAP"
BASE COUNT         1134 a         1797 c         1666 g         1264 t
ORIGIN      
        1 gctacgcgcc cagccccacc ggcgagctgc acctgggcaa cctgcgcacg gcactgctgg
       61 cctggctgct ggcgcgctgc gcgggcgggc agttcgtcct gcgcgtcgaa gacctggatc
      121 atccccgggt gcggcctggc acgacggagg gaatgctgcg cgacctgcgg tggctcggac
      181 tcgattggga tgagggaccg gaccgcggcg gaccatacgc gccctacacg cagagcgagc
      241 ggcagaccat ctacgcgcag cacctgcgcc gcctgcaaga ggccgggctg ctctacccct
      301 gttattgcag ccgcgccgac cttgcccgcg ccgccagcgc gccgcagggc gacgagggac
      361 cgcgctaccc cggcacgtgc cgcaacctga cggaggcgca gcgccgccag cgcgaggcca
      421 acggacgccg cccctcgctg cgtttccgcg tggctgaaga gcgcgtcgtc acgttcgatg
      481 atatgctgtt gggccgccag gaacagcaca cgcagcgcgc cgtgggcgac ttcatcgcgc
      541 tgcgttccga cggcatcttc tcttaccagt tcgccgttgt cgtcgatgac gccctgatgc
      601 gcatcaacca ggtcgcgcgc ggcgctgacc tgctctcctc taccgcgcga caaatcttgc
      661 tctacgaagc gctcggcttc tccatcccca ccttcgcgca cgttccactt atgctggatg
      721 ccaacggtca gcgcctcgcc aagcgccagc agagcgccgg cctggaacca ctacgcgccg
      781 ccggtgtctc gcccgcatcc gtcgtcggcc agcttgccgc gagttgcggt ctcattgcgc
      841 cgggtacgca gattagcgcg aatgagctag ctgagcagta tagtggggca ggatatgata
      901 tgatgcggac tgcatttttg cccccgattt acagaagtaa ggcaaggttc aaaaggtctt
      961 gaccgttcct ctccacctca ttcacctcct gaatttctct caggcacttt caggtaccta
     1021 cttaccaggt gagagaatac catgctatag tatccttagc tggtaggaac gtcaataccg
     1081 gtaatatagc agtgtcccag gtaagtgata atatggacgc acgcatcaga aaggaagaga
     1141 acatggcaaa gaaatgtacg catcttgatc agatacaaga agtcacaccg agcgccaatg
     1201 gatgtgagga ctgtttgaaa attggagata cctgggtaca tctgcggctc tgtcttatct
     1261 gtggacatgt gggctgctgc gactcttcaa agaacaaaca cgctaccagg catttccacg
     1321 ccaccaacca tccgatcgtt cagtccttcg aacctggaga ggactgggtc tggtgttaca
     1381 tcgaccaggt gatgctcgag cctgcttaat cgatgctggc tgcgctccag gcagcgagcc
     1441 gaacaccgtg aagccctcga ggtgcgaagc tgtggagcag agagacaatc aggtggagta
     1501 acacatggag aacataagca acacacagca aaaggaacga ggggaaggct ggccagcccg
     1561 tccctatgag gcatggaggg acacctgcca gactctgcat ctgtggacac agatcgtggg
     1621 caaagtcaga atggaactga gtccctttct caaccactgg tggcatgtca ccttctatgt
     1681 caccccgcga gggctgacca cctcttccat tccctatcaa gggggtgtct ttgaggtcat
     1741 atttgacttc gtcgagcaca atctctgcat ccgcaccagc gagggaacct ccagagcgct
     1801 gccgctgatc ccccgttccg tcgccgcgtt ttaccgggaa ttcatggcct gcttgcacgc
     1861 cctgggcatc gaggtcacca tcaacccctt gcccagcgaa gtgcagaatc ccattcgctg
     1921 tgatgtcgat gaggtccacg cctcctacga cccagtctac gcccagcgct tctggcgcat
     1981 tgtggcgcag accgatgccg tcttgcagcg ctaccgctcg ccgtttctcg gcaagagcag
     2041 tcctgtccac ttcttctggg gcagcttcga cctggcgctc accttttttt ctggtcgacg
     2101 cgcaccagag cgtccagggg ccgatcgcat gacgcgggaa gcctactcgc atgaagtgat
     2161 cagttgtggg ttctggcctg gcgatgaccg tttcccaaca ccggctttct actcgtatac
     2221 ctcaccagag ccgcctggac tgccggcagc ctcgattcgt ccggcggagg ccatctacaa
     2281 tcacgagctg ggagagttct tcctgcgcta tgacgacgta cgaacagctt cctcgcctga
     2341 gctggcgctg ctggagtttt ttcaaagtgc ctacgaagcc ggagccagac ttgcgcagtg
     2401 ggatcgcgag gcgctggaaa ggaaggcaat atgagaacca tcggtcatga aaaagcatag
     2461 gaaacaagcc gcctgccgtg atataatgcg gactgaaata atatattcgt aacctggaga
     2521 acgggttgcc gtcatacata atgatgaact ttcctgacca ggatcgggga gagtttgtca
     2581 ccctgagcgc agcgaagggt ctggctcatt aggcggagag atccttcgct gcgctcagga
     2641 tgacaggatc ggctttggtt gggaaagttt gtttatgatg gccacccctg gaaacaacat
     2701 ttttctatgg cgttattacc atttttctat ggtgagtcaa tggcaggtat cgttttgagc
     2761 gtcgttctta ctatactggt tgtcgcggtt gccctgggag tcgcgttttg gctgcgacgc
     2821 ctgatcgtgc gccgcttgaa gaaaaccgtt ctcgacaact ggcttgtcca gaccattggc
     2881 gcgcttgtca ccatccctgt tatcatcctg ggcctcctac tggtttcact caccgttact
     2941 atgagcgtca acttcatcat cacgctatgg agtggtatca ccgcggggct acaggaacag
     3001 accatcgcta atggcattcg cgcgcttctc tgggatttta ttgtcacaat tctcattatt
     3061 attctcgcta tcggggtcgg gcgcacggtg atgcggctga tcgtcggtcg gttgggggag
     3121 cagcgcctgg acatcaatat tcgcacgctg atcgggcgca ttttctatgg tctgattgtc
     3181 atggtctccg tcttctggat tctctcgctc tggcaggtcg ccatcgatgt cccgatcgcc
     3241 gtgattagcg cgctgagtgt ggccttcacc ttttccatcc aggacatcct gaaagacctg
     3301 gtggccggcc tctacatctt gttcgagcgt cccttccaca tcggcgagca gatcaccacc
     3361 gcgacctata ccggcaccgt cgaaaacgtg gagttgcgcg ccacaaaaat acgcctgacc
     3421 tccggcgaag agatcaccat tccgaacgcg ctggtcttcg gcggcatcgt cgtcaacaac
     3481 tcgcagtttg cagagcggcg cgcctccatt acgctgacca tgccgcagga ggaattcgac
     3541 aaagacgaga cgccgccgac cattctcgat gttgtgcgca agcaagaaaa cgtgctggtc
     3601 aagcctgaac cgcacgtctt tgtcaaccat tacgcgggaa ccttcggcgg gacaaccggc
     3661 gtaaaaacgg gctacagcgg ccagatcgtg gtcttgacgc tgcgcttctg gatgccgtct
     3721 ggccgctatg ataccgtgac ggaggtcatg agtgcgctgc acgaggcgct gccgacggtg
     3781 gacctgctgg tcgaggactc cggcggcaat gtctaggtgt tccttttagg agcggtaatt
     3841 cgttgcaata taataaatcg ttcccagtca cgcggcgcat cggcgtgatt ggcggtacct
     3901 ttgacccggt gcattacggg cacctggtga ttgccgagga ggtctacgcc gcgctcgacc
     3961 tggaggagat ggtctttgtg ccgacggggc agccgccaca caaaccggac cgcgtagtga
     4021 cgcccgcgga acatcgcctt gccatgctcg aacgcgccat cgccggcaac ccgcacttct
     4081 cgctgtcgcg ggtggacgta gaccgcgcgg ggccatccta taccgtcgag accctgcgcc
     4141 tgctgcgcca gcaatgggag gctcaggcgc aacaagctga cgatgtggag cgtatagagc
     4201 ttagtggatc aatcgcgctc tatttcgtga taggttggga cagcctggaa tacctgctta
     4261 cctggcatga cccggcaggt atccttgaac aactggacta ccttgtggca gtcaaaaggc
     4321 cgcgctacaa tgaagatggg acgatctacc tggacgcgct ggaaacgcgc ctgccgggtc
     4381 tcaagcagcg attgctggtg gtatccgcgc cgcagctgga catttcatcg accgatctgc
     4441 gcgcgcgggt cgcccagggt cgcccgatca agtaccagtt gccggagagc gtcgaagagt
     4501 atattagaga gtacggttta tatagccatg ccggataatc agtcgccgat gcatattgac
     4561 ctgggagatc gcttgcgcct gcgcaagcca catccctgcg gcggttacga ctggctggtc
     4621 gtgcgcctgg gggcggatat tggactgcgt tgcgaaaaat gcgggcgtcg tgtattattg
     4681 cctcgtgcag aagtcgaacg acgcaccaaa caggtgctaa cgcgcgcaat ggatgcttga
     4741 gacgagagca aggagatcga gttatggcgc ttgccaatcc acaaggaggc ttcctgacgc
     4801 tgctcaagaa gcgaaacttc ttgctgctgt ggctggcgca gttgatctcg atgaccgtgc
     4861 tgaatgcctc gaattacgcc ttgatcgtgc tgatcgaagt cattaccggc tcaaccacac
     4921 tgatcggcct ggctatcgtg tgcttcagcc tgcctgccgt gctcttcggc gcgcccgccg
     4981 gtgtcttcgt cgaccatcgc aacaagcgcc gcgtcctctg gtatagcaac tgcctgcgcg
     5041 ccatcgccac cttcggtttc gtcctctcgc tgctgctcac cagccacctg tctaccagtc
     5101 tccagcttat cccggtttac ctgctgacct tcctgatttc cggcatcggc cagttcttct
     5161 cgcccgccga gggcgccagc atccccctgc tggtcagcga agaagaactc atgcctgcgc
     5221 tctcgctctt caacatcacc ttcatgctag cgcaagcctt cggcttcatt atcttcgccc
     5281 cgatcctgct gagcgttctg ccaaccttca cgctctttag tgtcagcgtt ggcgcggtgc
     5341 ccacgctcta cgccatcttc gctctgctgt acctggtctg cgccgtgctg gtatcgctca
     5401 tcccggcgca gagcttcgag gaaaacggac acgcgccacg ggacagggaa acgatcggta
     5461 cgcaatcgct gggcgccttc agcaatgtga tgagtgaaat gcgccagggc tggcaattca
     5521 ttcgccgcca gcccccgctc ttcttgccgg tcattcagct ctccttcgcg ggcatcctgc
     5581 tgctggtcat cggcgaactc gccacgccgc tcgtcacccg gctcttcttg ctcccggcca
     5641 gctcgatggc ggtaatcttc gcgcctgccg gcctcggcct ggtgatcggc tcgatcttca
     5701 tgccgcgcgt cacccgccgc atcggcaaat cgcgcacgat cttcatcggc agtgtcagcc
     5761 tcgcgagtac tatcgtgctg ctgccgctgc tttcgtggct aaccaccacg ctgatcgcgc
     5821 acggcatcaa cttccgcccc gcgcaattcg ctatcgcgcc c
//
LOCUS       DASZAK010000277         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_468616, whole genome shotgun sequence.
ACCESSION   DASZAK010000277 DASZAK010000000
VERSION     DASZAK010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468616"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VGT44_04800"
     CDS             <1..662
                     /locus_tag="VGT44_04800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2580153.1"
                     /translation="PRAVHVIPCGVDLACFAPHDRREAREQLGWNPDSHILLFAGRLD
                     PFKGPDLLLRAAAMMEEKAEVVIVGGKPGDAEIHSLRELAADLKLRRRAHFPGAQSQQ
                     RLSLMYSAADVTVVPSYHESFGLVAVESLACGTPVVATRAGGLTTIVRHGETGYLIPR
                     CPGFFAATLDGLLQQPDLLASMRQAARPSVLHFSWTEIGQQVRDLYDDALDESQCLAA
                     L"
     gene            complement(733..1854)
                     /locus_tag="VGT44_04805"
     CDS             complement(733..1854)
                     /locus_tag="VGT44_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580154.1"
                     /translation="MKPFDDLLPAESETRHRELLTMLQRAYLKPAALTADEHDQAITR
                     VRDRLLTADQQADEDIPAPQAGVAYSAPIPATAMPRRRRRIINLLNSLAAVLVVGAIV
                     GASLLLFTRHAASPGGAPASTPSSIAIFVDLPIVASSSETGLEMTLAMTPGPYFLSEL
                     LAVVISLTNHSSKTYLVGIPFVSSACGYATGLSLSGGEKPYFDIPISIDHSCPAGSGG
                     VTLQPGKTLTALVYFPLMVSGHVSLSATTEFFDTSNNPQSPFRRPASNPLFKSWPTAR
                     FDVSAKIPADRALTFKRQGIHVTVSLPPQAQSPLIYLYGVFCQDSNNDGGSTGTGNFG
                     WYPISGNQVTQPACPGIHVQWRFALGLPGYGIAEGRVTS"
     gene            complement(1851..2393)
                     /locus_tag="VGT44_04810"
     CDS             complement(1851..2393)
                     /locus_tag="VGT44_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2580155.1"
                     /translation="MEQLRAEEAQNAANAAFYDRFAPAIFAYLYQQVASRQDAEDILL
                     EVFMAAQRQDALYGLGDEQRLTWLKRVARNKVIDCYRHSALLPLLPLDRAVEQEDRAL
                     TPEQQVVQRESYERLYQAIEQLSLEQQQLIRLRYGNGLRLVEIAEMLEKPDGTVRKML
                     ARALQQLRAHYEQAEKGTQR"
BASE COUNT          509 a          738 c          755 g          471 t
ORIGIN      
        1 ccccgcgcgc cgtacacgtg atcccatgcg gcgttgatct cgcctgtttt gccccgcatg
       61 atcgccgcga ggcgcgcgag cagctgggct ggaaccccga cagccatatc ctgctcttcg
      121 ccgggcgact cgatccattc aaaggacccg acctgctgct gcgcgcggcg gccatgatgg
      181 aggaaaaggc cgaggtggtc atcgtcggcg gcaagccagg cgacgcggag atacactccc
      241 tgcgcgaact ggctgccgac ctcaaactgc gcaggcgagc gcattttccc ggcgcgcaat
      301 cgcagcagag gttgtcgctg atgtacagcg ccgccgacgt gacggtcgtg ccttcctatc
      361 acgaaagctt tggcctggtg gcggtcgaat cgctggcctg cggcacgccg gtagtagcga
      421 cgcgcgccgg tggcctgacg accatcgtgc ggcacggcga aaccggctac cttatcccgc
      481 gctgccccgg cttcttcgca gccacattag atggcttgct gcaacaaccc gacttgctcg
      541 catcaatgcg ccaggcggcg cggccatcgg tgctgcactt tagctggacg gagataggcc
      601 agcaggtacg cgacctgtat gatgacgcgc tggacgagag ccagtgcctg gcagcgctgt
      661 aacccatgcg aaacacgcgc ttcatccgga ggggtagtat cgggatatac aggcgttttt
      721 ggaattagca gcctaagaag taaccctacc ttccgcgata ccatagccag gcaggccgag
      781 ggcaaagcgc cactgaacat gtatgccagg gcaggctggc tgagtcacct ggttccccga
      841 gatgggatac catccaaagt tgccggtgcc ggtcgagccg ccatcgttgt tcgagtcctg
      901 gcagaacacc ccatagagat aaatcaaagg ggactgcgcc tgtggaggca gcgataccgt
      961 gacgtggatg ccctgccgct tgaatgtcag cgcgcggtcc gccggtatct tcgcgctgac
     1021 atcgaagcgc gcggtcggcc aggatttgaa gagcggattg gaagctggcc tgcggaacgg
     1081 cgactgtggg ttattgctcg tatcgaaaaa ttccgtagtg gctgacagcg agacatggcc
     1141 gctgaccatt agtgggaaat acaccagcgc cgtgagcgtt ttgcccggtt gaagcgtcac
     1201 acctccagaa cccgcaggac agctatgatc gatagagatc ggtatatcaa agtacggttt
     1261 ttctcctcca ctcaagctca aaccagttgc atacccacac gcgctgctga caaatggaat
     1321 cccgacaaga taagtcttcg acgaatggtt ggtgagtgag atgacaacag ccagcaactc
     1381 gctgagaaag taggggccag gcgtcattgc caacgtcatt tcaagtccag tctccgagga
     1441 actcgccact ataggaaggt ctacgaagat cgcaatcgaa ctcggcgtcg aagcaggcgc
     1501 gcctcctgga gaggcggcat ggcgcgtaaa aagcagcagc gacgcgccga caatggctcc
     1561 gacgaccagc acagcagcca ggctattgag tagatttata atacgcctac gacggcgggg
     1621 catcgcggta gccgggatag gggcagaata ggcaacgcca gcctgcggtg cgggaatgtc
     1681 ttcgtcggcc tgctggtcgg cggtcagcaa gcgatcccgc acgcgcgtga tggcctgatc
     1741 gtgctcgtcc gcggtcaaag ccgccggttt gaggtaggcg cgttggagca ttgtaagcaa
     1801 ttcccggtgt cgagtctcgc tctctgccgg caaaagatca tcgaatggtt tcatcgttgt
     1861 gttcccttct cggcctgctc gtaatgcgcg cgcagttgtt gcaaggcgcg cgccagcatc
     1921 ttgcgaaccg tgccatccgg cttctccagc atctcggcaa tttcgacgag gcgcagaccg
     1981 ttgccataac gcaggcggat caactgctgc tgctccagag aaagttgttc gatggcctgg
     2041 tagagccgct catagctctc gcgctgcacg acctgctgtt cgggcgtcaa cgcccggtcc
     2101 tcctgctcca cggcgcggtc gagcggcagc agcggcagca gcgcggaatg ccggtaacag
     2161 tcgataacct tattgcgagc gacgcgcttc aaccaggtca atcgctgctc atcgcccagg
     2221 ccgtagagcg catcctggcg ctgggcagcc atgaacacct ccagcaagat gtcttccgcg
     2281 tcttgccggc tggcgacctg ctggtacagg taggcaaaga tggccggggc aaaacgatca
     2341 tagaaggccg cattcgccgc gttttgcgcc tcttccgccc tgagctgttc catgcgctcc
     2401 cccttcgttg tatgagatct catacagtat gtcgtatgaa cgagcaaaac gtggcatgat
     2461 tgcgacgtgg caa
//
LOCUS       DASZAK010000278         3789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_575091, whole genome shotgun sequence.
ACCESSION   DASZAK010000278 DASZAK010000000
VERSION     DASZAK010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575091"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VGT44_04815"
     CDS             <1..589
                     /locus_tag="VGT44_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580156.1"
                     /translation="GGICVNEWLIREGYLTLKSMPTSLTAIDKCEIDWSRTKVWGDGG
                     YYARIFLNIEGREPQGIVKAEDVEALRSELKAKFEAIVDPDGVNIGTTVYKPRDIYHE
                     VNGVPPDLIVYFGDLFWRSIGTVGHGSVYSFENDTGPDDCNHAQFGIVVKHDPDEDMG
                     PGGHELHGLQLMDMAPTILKQLDVPVPADMQGRAF"
     gene            608..1219
                     /gene="cysC"
                     /locus_tag="VGT44_04820"
                     /pseudo
     CDS             608..1219
                     /gene="cysC"
                     /locus_tag="VGT44_04820"
                     /inference="COORDINATES: protein motif:HMM:NF003013.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
     gene            1283..2449
                     /gene="sat"
                     /locus_tag="VGT44_04825"
     CDS             1283..2449
                     /gene="sat"
                     /locus_tag="VGT44_04825"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEV2580157.1"
                     /translation="MENKLIAPHGGELILNMASEQEQAELREVARALPRIVVGSRQLA
                     DLEMLAIGAYSPLSGFMTRGDYQGVVDGMHLSNGLPWSIPITLSAGAEQAATLKEGSQ
                     VALVNTQGTLQAVMTLHEKFAYEKQHEAGKVYRTTDEAHPGVKVLYEQGDVLLGGPVR
                     VVALQEQAFASYRFTPTQSRSMFAERGWKRIVGFQTRNPVHRAHEYIQKCAMETVDGL
                     FLHPLVGDTKGDDIPADVRMRCYEVLLENYYPADRAILGVLPAAMRYAGPREAIFHAL
                     MRKNYGCSHFIVGRDHAGVGSYYGTYDAHYIFAEFDAAKLGITPMFFDHTFFCRACDS
                     MASSKTCPHGNEHHVTLSGTKVRQMLLAGEIPPREFSRPEVAKVLIEAMRQPVA"
     gene            2559..3638
                     /locus_tag="VGT44_04830"
     CDS             2559..3638
                     /locus_tag="VGT44_04830"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580158.1"
                     /translation="MLIARAPMRISFGGGGTDLEAYYARFGGLVISTAINKYFYAIIT
                     TDESEDLQVISADYRSLFRHTPYNDLFWNGDLALPKAVLHHFGIRRGINLFVASEVPP
                     GTGLGSSSAAAVTLVRAISTLVEQPMTKQQVAELACAIEIGKMGMPIGKQDQFAAAFG
                     GLNKIIFSEEGVSVEPLHIAHEVRQTLERRLMLFFTGSSRESTSILKHQRKSTEDRDD
                     SVLQALHNIKQVAVDVQACLEVGDLDEFARLLDYAWQEKRRLAPGLSTSTIDEYYRLA
                     REQGAAGGKITGAGGGGFLLLYCVEDRQLAVTEALERHGLKRMNFHFDQQGATVLMNV
                     ANFNNLWVAPYADHPTGELALQSGA"
BASE COUNT          808 a          978 c         1212 g          791 t
ORIGIN      
        1 tggcggcatc tgcgtcaatg aatggctgat ccgcgagggc tatctaacac tcaagtcaat
       61 gccgaccagc ctgacggcga tcgataaatg cgagattgat tggagccgca cgaaggtctg
      121 gggtgacggc ggctactatg cccgcatttt cctgaacatc gaagggcgcg agccgcaggg
      181 tatcgtcaag gccgaggatg tcgaagcgct gcgcagcgag ttgaaggcga agttcgaggc
      241 catcgttgac cccgacggcg tcaacattgg cacgacggtc tacaagccgc gtgatatcta
      301 ccacgaggtg aacggtgtgc cgcccgacct gatcgtctac ttcggcgacc ttttctggcg
      361 ctcgatcggc actgtcgggc atggctccgt ctactcgttc gagaacgaca ccggcccgga
      421 cgattgcaac cacgcgcagt tcggcatcgt cgtcaaacat gatcccgacg aggatatggg
      481 gcctggcgga cacgaactgc acggtttaca attgatggat atggctccga ccatactcaa
      541 acaactcgac gtacctgttc cggcggacat gcagggcagg gcattttaag tcaaagtaac
      601 ctggaggaag aaacgttata tgtcaacgaa taaaggtttt acgatctggt tcaccggcct
      661 gtccggagcg ggcaaaagca cgctttctga ggctatccac gaacgcctca aggcgcgcgg
      721 gcgcaatgtg gaggtgcttg acggcgatat cgtgcgcacg cacctcagca aggggctggg
      781 cttcagcaaa gaggatcgtg atacgaatat caagcggatt gctttcgtct gcggcctgct
      841 gacgcgcaat ggcgtgatgt gcatctctgc ggctatctcg ccctatcgcg aaacacgtgc
      901 gtgggcgcgc gatcacatcg gcaactttgt cgaagtctat gtgaaatgtg atatcgctgt
      961 ctgccgccag cgcgacgtga agggtctcta taagctggcg gacgagggca agataaaggg
     1021 atttaccggc gtagatgatc cttacgagga gccggactat gctgagctgg tcgtcgagac
     1081 ggacaaagaa agtattggag agagcgtcgc gcgcattttc gcaaagctcg aggaactggg
     1141 ctacctggag ccggaagaat ccagcgacga ggactcgaag gttgtcaccg atcgcttgat
     1201 ggcgctcggt tatctataga atcccgtagg agcgcggtaa accggctcct acgagggttg
     1261 tagaggttag gaagagagca agatggagaa caagttaatc gctcctcatg gaggagagct
     1321 gattttgaat atggcgagcg agcaagagca ggcggaactg cgcgaggtag ctcgtgcgct
     1381 cccacggata gtggttggtt cgcgccaact ggccgacctg gaaatgcttg cgattggcgc
     1441 gtatagcccc ctgagcggct ttatgacgcg cggggattac cagggcgttg ttgatgggat
     1501 gcatctcagc aatggcctgc cctggtctat ccctattacc ctgtctgccg gcgcggagca
     1561 agcggcgacg ctgaaggaag gctcgcaggt cgcgctcgtc aacacgcaag gcacgttgca
     1621 ggcagttatg acgctgcacg agaagttcgc ttatgagaag cagcacgagg ccggcaaagt
     1681 ctatcgcacg acggatgaag ctcatccagg cgtaaaggtg ctgtacgagc agggcgatgt
     1741 gttgctgggt ggaccggtgc gcgtggttgc gctgcaagag caagcgtttg cgtcctaccg
     1801 attcacacct acgcagtcgc gcagcatgtt cgcggagcga ggctggaagc gcatcgtcgg
     1861 gttccagacg cgcaaccccg tgcatcgtgc ccacgagtat atccagaaat gcgcgatgga
     1921 gacggtagat ggcctcttct tgcacccgct tgtcggtgat acgaaaggcg acgatattcc
     1981 tgccgatgtg cggatgcgct gttacgaggt gctgctggag aactactatc cggcggatcg
     2041 tgcaatcctg ggggtgctgc ctgccgctat gcgctacgcc ggcccgcgcg aggccatctt
     2101 ccacgcgctg atgcgcaaga actatggttg ctctcacttc atcgtggggc gtgaccatgc
     2161 gggcgtcggc agctactacg gcacgtatga tgcgcattat atctttgccg agtttgacgc
     2221 ggcgaagctg ggtatcacgc cgatgttctt cgatcatacc ttcttctgcc gcgcctgcga
     2281 ctcgatggca tcgtcgaaga cctgtccgca tggcaatgaa caccatgtca cgctgagtgg
     2341 cacgaaggtg cgccagatgc tgctggcggg cgaaatcccg ccgcgcgagt tctctcgtcc
     2401 agaggtcgcg aaggtgctga ttgaagctat gcgccagccc gtcgcataag ggcattccat
     2461 cgagatgcct gcgtcgccta tgtatcaggt gagtaggcga cgcaggcact catatctaat
     2521 tggaacggtt tgaaagggtg catgtaggag agacagtaat gcttatagct cgagcgccaa
     2581 tgcgtatcag ctttggcggg ggtggaaccg acctggaagc gtattatgcc aggtttggcg
     2641 gactggtcat cagcacagcc attaataagt acttttatgc cattatcacg acggatgaaa
     2701 gcgaagattt gcaggtgatc tcagctgatt atcgctcgct gtttcgtcac actccttata
     2761 atgatttatt ctggaatggc gatctggcgt tacccaaggc ggttttacac cacttcggca
     2821 tccgtcgcgg cattaacctg tttgtggcca gtgaggtgcc gcccggcacc ggcctcggct
     2881 cttccagtgc tgcggcggtg acgctggtac gcgctatctc gacactggtt gagcagccga
     2941 tgaccaagca gcaggtggcg gagctggcgt gcgccattga gattgggaag atggggatgc
     3001 caataggcaa gcaggatcaa tttgcggcgg cgtttggcgg gttgaataag attatcttca
     3061 gcgaagaggg tgtcagcgtt gagccgctgc atatcgcgca cgaagtgcgc cagacgctgg
     3121 aacgccgcct gatgctgttc ttcaccggta gttcgcgcga atcgacttcg atcttgaagc
     3181 accagcgcaa atcgaccgag gatcgcgacg atagcgtcct gcaagcgctg cacaacatca
     3241 agcaggtggc ggttgatgta caggcatgcc tggaagtagg tgacctggac gaatttgcgc
     3301 ggctgttgga ctatgcctgg caggagaaac gccgcctggc tccgggcctc tccaccagta
     3361 ccatcgatga gtactatcgc ctggcgcggg aacagggggc ggcgggtggc aagatcaccg
     3421 gcgcgggcgg gggtggtttt ctgctcctgt attgcgtgga agaccggcag ctggcggtca
     3481 cggaggctct ggagcggcac ggcctcaaac gcatgaactt ccattttgat cagcaggggg
     3541 cgacggtgct gatgaacgtg gcgaacttca ataatttatg ggtggcgcct tacgcggacc
     3601 atccgacggg tgagctggcg ctgcaatcgg gtgcgtaaga gtgtctatga gagagccggg
     3661 tcgccctggc cagtcaagaa aaagggggaa gaatcttgat ggagaacgtc ttaagctact
     3721 ttggggagct ggagcagatg ctgcgcgcta tctcgctgtc tgatctgcgc ggaacgctgg
     3781 acattctgg
//
LOCUS       DASZAK010000279         2483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_575107, whole genome shotgun sequence.
ACCESSION   DASZAK010000279 DASZAK010000000
VERSION     DASZAK010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575107"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..>2483
                     /locus_tag="VGT44_04835"
     CDS             100..>2483
                     /locus_tag="VGT44_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2580159.1"
                     /translation="MILKGLLALLTQRPETRRLAQQIQQAGGLPAITGVTETARPFVT
                     AALSAALKQPVLLVVSDEAQAMLVTETLKAFAQQPEEVFYLPDRDALPYERLMNDAET
                     TQQRLNALIRLTEHHAQTVVVCSARALTQPIVPPQELAASLYELVPGQEVDLTSLLEH
                     LYNLGYEPVTEVEEAGQFSHRGGIVDLFPPTLPRPVRIEFFGDEIESLRTFDPETQRS
                     LNPLTTFVVAPAREALPTRGPEAARALAQLNTAMLHPDADERWKRDLEDLRASRSFDD
                     IIFYIAYLHPQASVLDYLPKSGLLILDNPESLQRTITDLDAQALEMKERFEQDRENPT
                     QLRPAHISWDGLETRLAQMRQLRFAGMLSASEGEFDAQQHGGTEHLIPVFSSASSYGG
                     RLRAFVQECRRALDHRERVVIVSAQARRLAEVLGDDAILGEKAIHVSPGVNINEPPES
                     GALTLIQGQLPEGWQSQSLGLHFYTDTEIFGWSRKYKSQQRKVVTPAAFLAEVNPGDH
                     VVHQEYGIGRFEGLVKLNSTGVEREYLLIHYAGTDRLYIPTDQLDRVTRYVGMGEAVP
                     ALSRLGTSEWTRAKTKAKESAQDIARDLLRLYSARESLERPVYPPDSEQPWLQELEAG
                     FPYEETPDQLRAIEEVKADMERPKPMDRLVCGDVGYGKTEVALRAAFKAVLDQRQVAV
                     LVPTTVLALQHFNTFQERLKPYPVRVELLSRFRSEKEQKQILDDLAIGKVDIVIGTHR
                     LLGKDVVFLNLGLLVVDEEQRFGVTHKERLKELRNEVDVLTMTATPIPRTLHMSL"
BASE COUNT          509 a          775 c          765 g          434 t
ORIGIN      
        1 agaaacaaag acatacgcta acaacaatgc aaggcgagtc tgccgggatg ggtagttttc
       61 ccggaaggcg cattttgcta tgctgttttg gaggggtttg tgatacttaa agggctactc
      121 gcgcttctca cccagcggcc agagacgcgc cgcctggcgc aacagataca acaggccggg
      181 ggattaccgg ccattacggg cgtcacggaa acggcgcgac ctttcgtgac agccgcgcta
      241 tcagcagcgc tgaagcagcc tgtgttgctg gtcgtctccg atgaggcgca ggctatgctg
      301 gtcacggaga cgctcaaggc attcgcacag cagccggagg aggtcttcta cctaccggac
      361 cgtgacgcgc tgccctacga acgactgatg aacgacgcgg agacgacgca gcagcgcctg
      421 aatgccctga tacgcttgac ggagcatcac gcgcagacag tagtcgtctg ctcggcgcgc
      481 gcgctgacac aacccatcgt cccgccacag gaacttgcag cctcgctcta tgaactcgtg
      541 ccggggcagg aggttgacct gacctctttg ctcgaacacc tctataatct gggctacgag
      601 ccggtgacgg aggtggaaga ggcgggacag ttcagccatc gcggcggcat cgtcgatctc
      661 tttccgccca cgctgccgcg cccggtgcgc atcgagtttt tcggggacga aatcgaatcg
      721 ctgcgcacgt ttgacccgga gactcagcgt tcgctcaacc cgctcaccac cttcgttgtc
      781 gctccggcgc gcgaggcgct gccgacgcgg gggccggaag cggcgcgcgc gttggcgcaa
      841 ctgaataccg ccatgctgca ccccgacgcg gacgaacgct ggaagcgcga cctggaagac
      901 ctgcgcgcca gccgctcgtt cgacgacatc atcttctaca ttgcctacct gcatccccag
      961 gccagtgtgc tggattacct gccgaaaagc ggcctgctca tcctggataa ccccgaaagc
     1021 ctccagcgca cgatcacaga cctggacgcc caggcgcttg agatgaaaga acgctttgag
     1081 caagaccgcg agaaccccac tcaactgcga ccggctcaca tttcctggga cgggctggag
     1141 acgcgcctgg cacagatgcg ccagctgcgc ttcgcgggga tgctctcggc cagcgagggc
     1201 gaattcgacg cccagcagca cggcggcacg gaacacctga tccccgtgtt cagcagcgct
     1261 agttcgtatg gcgggcggct gcgcgccttt gtccaggaat gccgccgcgc gctcgatcat
     1321 cgcgaacgcg tcgtgatcgt cagcgcccag gcgcgcaggc tggcggaagt gctgggcgac
     1381 gatgccatcc tcggtgagaa agccatccac gtcagccccg gcgtgaacat caacgaacca
     1441 ccagaaagcg gcgccttgac gcttattcag ggacaactgc cggaaggttg gcaatcgcag
     1501 agcctgggtc tgcatttcta taccgataca gagattttcg gctggtcgcg caagtacaaa
     1561 tcgcagcagc gcaaggttgt gacaccggct gcattcctgg ccgaggtcaa tcctggagat
     1621 cacgtagtcc atcaagagta cggcatcggc cggttcgagg ggcttgtcaa gctcaattcg
     1681 accggcgtgg agcgcgaata cctgctgatt cattacgcgg gaaccgaccg gctctatatc
     1741 ccaaccgacc agcttgaccg cgtcacacgt tacgttggca tgggcgaggc ggtccccgcg
     1801 ctgagcaggc ttggcacatc cgagtggacg cgcgcgaaga cgaaggccaa agagagcgcg
     1861 caggacatcg cccgcgacct gctgcggctc tacagcgctc gcgagtctct cgaacggccc
     1921 gtttatcctc ccgattctga gcagccctgg ctgcaagaac tggaggcagg cttcccctac
     1981 gaagagacgc ctgaccagct gcgcgccatc gaagaagtca aggcggacat ggagcggcct
     2041 aaaccgatgg accggctggt gtgcggcgac gtgggttatg gcaagaccga ggtcgccctg
     2101 cgcgccgctt tcaaagcggt gcttgaccag cgacaggtgg ccgtgctggt acccacgacc
     2161 gtgctggccc tgcaacactt caacaccttc caggagcggc tcaagcccta cccggtgcgt
     2221 gtcgaactgc tcagccgttt tcgttccgag aaggagcaga agcaaattct ggatgatctg
     2281 gcgataggaa aggtcgatat tgtgatcggc acgcatcgcc tgctggggaa agatgtcgtc
     2341 ttcctcaacc tcggcctgct ggtggtggac gaggagcagc gctttggcgt cacccacaaa
     2401 gaacggctca aagagttgcg caacgaggtc gatgtgctga ctatgacggc cacgccgatc
     2461 ccgcgcacgc tgcacatgtc gct
//
LOCUS       DASZAK010000280         2900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_362607, whole genome shotgun sequence.
ACCESSION   DASZAK010000280 DASZAK010000000
VERSION     DASZAK010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362607"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..276)
                     /locus_tag="VGT44_04840"
     CDS             complement(34..276)
                     /locus_tag="VGT44_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580160.1"
                     /translation="MMPNTHIHEQLMLQRCQELQRDRAQQRLASELRRHHPSLPGRFA
                     SAIGARLLALIARMKRREPGREPGNEPVVYEKSSAQ"
     gene            668..868
                     /locus_tag="VGT44_04845"
     CDS             668..868
                     /locus_tag="VGT44_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580161.1"
                     /translation="MNTRFFSRPFRFARRLVVTTTLLVVLGLPGVAIGQSASQAAFHT
                     RSAAPVSHVIMAHPKLAYHTHP"
     gene            complement(1063..>2900)
                     /locus_tag="VGT44_04850"
     CDS             complement(1063..>2900)
                     /locus_tag="VGT44_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV2580162.1"
                     /translation="AQKLLDDLEWLDWSERIKTNQRNWIGRSEGLEFAMEIDGTAGES
                     VQVYTTRPDTIYGMTFVALAPHHPLISRITAGERLAAVTAYQQTANRPHNDEEHTMTG
                     VFTGSYALHPLTGERIPIWVADFVLEGYGTDAIMGVPAHDERDMAFVKAVGLPVRIVV
                     VPPDDDAALLSEEMQQTGEAYVQPGILIDSGEYADMTSAQASDAIAAWFEERGSGKRV
                     AKYRLRDWLISRQRYWGPPIPIIYCPQHGAVPVPEEQLPVVLPDVENWMPTGTGVSPL
                     AAVEEFVNTTCPICGQPARRETDVSDNSLDSAWYFLRYLSHDDDSQAWDPALARKWLP
                     VAMYIGGAEHSVLHLMYARFITMALHDLGYLEFEEPFKRFRANGMITKDGAKISKSKG
                     NIVNPDDYITRFGADVFRVYLMFMGPYDAGGDFSDRGIGGVVRFLDRAWSLVTRHAGD
                     AHEGPAQGDARRDLHRAIKRVTEDLAALKYNTAVAALMELLNSLESRQNVTREELRTL
                     LLLMAPLAPYITEELWERIGQPGSIHAATWPAFDADALHAETMTLPVQVNGRVRGRIE
                     VAHDAPEAEIKQLALMATQVQSYTAGKQVSRVIYVSGRLVNIVIG"
BASE COUNT          543 a          910 c          856 g          591 t
ORIGIN      
        1 ccggtcatgg tgcgtcgtca tgcgaagtcc actttattgc gcgctactct tctcgtacac
       61 aaccggctcg ttgcccggtt ctctgccggg ctcacgcctt ttcatgcgcg ctatcaaggc
      121 gagcaagcgc gccccgattg ccgaggcaaa gcgacctggc aggctggggt gatggcgacg
      181 cagttctgac gccagccgct gctgcgcccg gtcgcgctga agttcctggc aacgctgtaa
      241 cattaactgc tcatggatat gggtgttcgg catcatggat tttctccttt tcaggcatct
      301 cgcaagtacg caacgggtct ttcttctcaa gtgaagaccc ttactcacag tgtatgtgga
      361 tgaagcccgg cggacatcgg aagaataacg tgttgcgtaa cgtatctacg cctggggcag
      421 aagggtatga ccggggacca tctacgtaga atcacgtggt cgaattgcgc agatgcggtg
      481 agatggcacc atatggagcc agaggaaata cgtcatcaca cacgttcttt tacggatacg
      541 ccgccgctct ccatacagta cactcgcaac agtgaatggt tcacaacggt gaaccgcagc
      601 gaagtaacga acccatcaac cgggttccac aacacacact gtccgtcatg gacaacagga
      661 ggattacatg aacactcgtt tcttctctcg ccccttcagg tttgcgcgcc gtctcgtcgt
      721 caccaccaca ctgctcgttg tcctcggcct gcccggagtg gccatcggcc agtccgcgag
      781 tcaggcagcc tttcatacga ggtcggcggc gcccgtctcg catgtgatta tggcgcatcc
      841 caaactggct tatcatacgc acccgtagcg ctcgcggtcc agggatgacg gctaacgaag
      901 gccattcctg atctgccatc tctacagagt ctggaaatga ttgctggggt acgcaaaggg
      961 cgaccacaag ggtacgccct taccatgact gcgaagtacg tttgcgcatc agcctccgtc
     1021 gtgtcatggt aggggcgcac ccttgcggtc gcccttcgta gctcatccga tcacgatatt
     1081 caccagcctc cccgacacat aaatcacgcg gctcacctgt ttccccgcgg tatacgattg
     1141 cacctgcgta gccatgagcg caagctgctt gatctccgct tcgggcgcgt cgtgcgcgac
     1201 ttcgatgcgg ccacgcacgc gcccgttgac ctgtaccggc agggtcatcg tctcggcgtg
     1261 gagagcgtcg gcgtcgaacg cgggccaggt cgccgcatga atcgaacccg gctgcccgat
     1321 gcgctcccag agttcttccg tgatatacgg agccagggga gccatgagta acagcagtgt
     1381 gcgcagttcc tcgcgcgtga cattctgcct gctttccagg ctgttgagca gctccatcag
     1441 cgcggcaacg gcggtattat atttcaatgc ggcaaggtct tcggtgacac gcttgatggc
     1501 gcggtgcagg tcacgccgcg catcgccctg ggctggaccc tcatgcgcat ctccggcgtg
     1561 gcgcgtgacc agcgaccagg cgcggtcaag gaaacgcacg accccaccga tgccccggtc
     1621 gctgaaatcg ccgccagcat cgtatggccc catgaacatc aggtagaccc tgaatacgtc
     1681 cgcgccaaag cgcgtgatgt agtcgtccgg gttgacgatg ttgcctttcg acttggagat
     1741 cttcgcgcca tccttcgtaa tcatgccgtt ggcgcggaag cgtttgaacg gctcctcgaa
     1801 ctcaaggtag cccaggtcat gcagcgccat cgtgatgaag cgcgcgtaca tcaggtgcag
     1861 cacgctgtgt tccgcgccgc cgatgtacat ggcaaccggc agccacttgc gcgccagcgc
     1921 cgggtcccat gcctgtgagt cgtcatcgtg ggacaggtag cgcaggaagt accaggcgga
     1981 gtccagggag ttgtcgctca catccgtttc gcgcctggcg ggttggccgc agatgggaca
     2041 ggtggtattg acgaactcct cgacggccgc gagcggcgac acgccggttc cggtgggcat
     2101 ccagttctcc acgtcgggca agaccaccgg cagttgctcc tccggcaccg gcacggctcc
     2161 gtgctgcggg cagtagatga tcggaatggg cggtccccaa tagcgctggc ggctgatgag
     2221 ccagtcgcgc agtcgatatt tcgcgacccg tttgccgctg ccgcgctcct caaaccaggc
     2281 cgcaatcgca tcactggcct gcgccgaggt catgtcggca tactcgccgg aatcaatcag
     2341 tatgcccggt tgtacatatg cctctcccgt ctgctgcatc tcctccgaca gaagcgcggc
     2401 atcgtcatca ggcgggacaa ccacgatacg caccggcagt cctaccgcct tcacgaaggc
     2461 catatcgcgc tcgtcatgcg ccgggacgcc catgatcgcg tccgtgccgt agccttcgag
     2521 gacgaaatcc gctacccaga tgggaatgcg ttcgcccgtc agaggatgca gcgcataaga
     2581 cccggtgaac acgcccgtca tggtgtgctc ttcgtcgttg tgcgggcgat tggcagtttg
     2641 ctggtaggcc gtgacagccg cgaggcgctc gcctgcggtg atacgtgaga tgagcgggtg
     2701 atggggggcc agcgccacga aggtcatgcc atagatggtg tccgggcgcg tcgtatagac
     2761 ctgcacgctc tcgcccgccg tcccatcgat ctccatcgcg aactcaagac cctccgagcg
     2821 gccaatccag ttgcgctggt tcgttttgat gcgctccgac cagtcgagcc attccaggtc
     2881 gtccaggagc ttttgcgcgt
//
LOCUS       DASZAK010000281        11015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_326807, whole genome shotgun sequence.
ACCESSION   DASZAK010000281 DASZAK010000000
VERSION     DASZAK010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 11015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11015
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326807"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..638
                     /locus_tag="VGT44_04855"
     CDS             258..638
                     /locus_tag="VGT44_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580163.1"
                     /translation="MNRLMRISLIVNIWRNSGVSELPFEENPLLPGAAGLMGISFSVN
                     EGHWPNVAFGKSLLNWVRQQSWLPPSVHTTCVIRLPKHCSILLPTDLTDPRCLFQLFS
                     NCLAMLILQRQPSTRGCLLMTWRG"
     gene            complement(866..1501)
                     /locus_tag="VGT44_04860"
     CDS             complement(866..1501)
                     /locus_tag="VGT44_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2580164.1"
                     /translation="MRILGCLDGTNTEQIRQAVSSLLTTSDRTIGLLYVTDAGPQADL
                     ERQRERFLRPPGLPPARREQMRQAEVASAQGILVEGGRAFPGAERIHRTGRPEREIVN
                     AAAEWNADLILICPRTNYDDKPIIGPKSVGHVARFVLDHAPVQCYWCVHRPETHSCEV
                     GGRRGVAVFHRHAKIPFWSFHKDDPFPAAPSRTGLASFDASDSPGLRLGLP"
     gene            complement(1511..2821)
                     /locus_tag="VGT44_04865"
                     /pseudo
     CDS             complement(1511..2821)
                     /locus_tag="VGT44_04865"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
     gene            complement(3069..3473)
                     /locus_tag="VGT44_04870"
     CDS             complement(3069..3473)
                     /locus_tag="VGT44_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV2580165.1"
                     /translation="MLLFFQIPALTKTIMVSFIAVCTLIGIMLRPFKLDEARIALGGA
                     ALLLLLGLIAPLDALTTLLSDWNIFFFFLGMMTLSAMADAAGLFDWLAAKAAGLSSGS
                     SRRLLFNIFLLGCLISMILSNDATALILTPIV"
     gene            3614..4531
                     /locus_tag="VGT44_04875"
     CDS             3614..4531
                     /locus_tag="VGT44_04875"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2580166.1"
                     /translation="MAQLGFVGLGNMGGGIVKRLLDAGHTVVGYNRTWSKAQWLLDAG
                     MQWGETPRQVAETADVTFSMVANSAALRDVFGGHDGILAGLGPGKIYVDMSTVSPSIS
                     RELSERVAAKGAQMLDAPISGNVITLVQGTSSIMVGGDQATFEKIKPILLDIGSKVNY
                     IGGNGQALLMKAAINLSLPIQFMGFCEGVLLAEKGGIPRETAIRVWLSSALVSPATEH
                     HALLLQEKSEEVMFNVNMMQKDMLLALELGRELDVALPAVALSNEILTAARAMGLARE
                     DYAIMFEVLAQLAGLNNESSAKGDQDASR"
     gene            4518..5393
                     /locus_tag="VGT44_04880"
     CDS             4518..5393
                     /locus_tag="VGT44_04880"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2580167.1"
                     /translation="MHHVRNYSNATFQVPPGYEKHSLDYSRVALVDHTTGSVHMGLGL
                     CKLEPEGTLEPHVHFYEEAFYILEGQVLGSIDGRVYRFGPGDYGIIQAAVTHAWRGVG
                     DQPARWLEMLSAQPKPPGGERDTFFLKGAAAPDEGNPPDLRNPLTRYLGHFDDQHMPP
                     PSQLQMDGYRGSNIYGISLKMLVDRMLGAQHLTLFMVEFQPGGDGNVHDHPFEESYFL
                     VSGEADTILDGKPYHVKAGDYVWTGVGSTHGFFNRGTVPVRWIETQSPQPPNQQAFRF
                     PSDWAYLAARLEDSE"
     gene            complement(5520..6041)
                     /locus_tag="VGT44_04885"
     CDS             complement(5520..6041)
                     /locus_tag="VGT44_04885"
                     /inference="COORDINATES: protein motif:HMM:NF024061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HEV2580168.1"
                     /translation="MHTPRRKLLRLEEQRYSRNQQDQFEDDWGDDERKERRKPRYNRS
                     SDEVFKCKRCRRFVCPPPSGGRHRNHCPFCLYSRHVDDKKPGDRMSPCGSLMEPVGYF
                     QRPNGEYVIVHSCFGCEVERFNRIAADDDFELVVSLPSQKPRTSREAKAERVEALLAE
                     QEGQWDWDAEEAG"
     gene            complement(5962..6180)
                     /locus_tag="VGT44_04890"
     CDS             complement(5962..6180)
                     /locus_tag="VGT44_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580169.1"
                     /translation="MLNTTIPSASVLALAPTGWQAKVAFAAGQPDQLLVNNRAKIKKR
                     ITDAYTTQEIAAFRRAKIFPQPTGPIRG"
     gene            6738..10490
                     /gene="smc"
                     /locus_tag="VGT44_04895"
     CDS             6738..10490
                     /gene="smc"
                     /locus_tag="VGT44_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV2580170.1"
                     /translation="MYLKRLEMLGFKSFAPRTMLEFSTGVTAIVGPNGAGKSNVADAV
                     RWVLGEQSMRQLRGKRGDDIIFAGGFGKAALGMAEVSLTLDNSTGWVPSEYSELTVTR
                     RSYRSGENEYLINKQKVRLKDVVLLLAQARIGHDSYTVVGQGLIDAALSLRAEERRGL
                     FEDAAGIRPYQVQRADSESRLRQTESNLERLRDIVNEIEPRLEPLAEQARRATEHRQL
                     NSELQDVLLTWYALQWRRLRATQERVEVEEQAMSGRVQHGERELQTFDERRDTLRQSR
                     QEAQNAIAQAREASNQCLASAQHIEREMAVGQERIVGLQRQRDEQQQEERRLRERLIT
                     ARQQLIDLEEQCELAEEGIDTDAAALATFEGQVAQAQKAYEMDERRLRNAQSELIQVQ
                     ARLGASQSDLGRQQKHLGERNRALAARRETIAQSQQATRAQETRLVEERARLDTARQE
                     EQQITQRKQSIGQQIGDAQQEIERFKQALNEAERQRRIVADRLNMLKNWRSSLSGYSD
                     GVRAILRAPSGKLGGLLGPIPQLGYAPAGLETALEAALGSYLQAVVVQSLDQAQACLG
                     FLQATNAGKAMIAWLDDDTYQANIGNYRQEVETLKRLLVDMPGMQARVRGLAWQLMQC
                     EPRYLPLFQRILRGAVVTQDLETARALLDRVVRMPANERHLETFVTLRGEVLHVDGWL
                     TGGSGKDGGQQGVLAYERELRELPEQLDRHVALINELNGNISEAQRAQEARRVEQSAI
                     EKELQRNASRVNEISKGVATTQRELDRLQSELQLARSVEQQLASEIAGLDQEVQAAVE
                     RVRAHEKSQREMTGLVEELQREVEERTLVFRRQQDDLGKARTALAVKRQEAKALRQQL
                     VAQQAAAQDLKAQIDQRVARAKEVETQLQALQTALEGQRARLEKNRTQSQQLLEALQN
                     AEARAGEFDTQLLELEQETLRARQALNELEVGYRQCLLASQKARDAVAALLEQLQEEV
                     GIADPAELVRYAARANVTLVDEAGGEEARPAQEESVVLSEEEEAQLRRLRRRVDTLRS
                     RLRALGGFDPDAPQQYEETRTRYEFLSTQIADMEQAALQLHSIIDQLDVTMARQFEIT
                     FQTVNARFAEHFTTLFNGGQARLELTVAKQEDEDGTLRANPAGMPAGVEVIVQPPGKK
                     VMDLSLLSGGERALVSAALLFALLEINPPPFCLLDEVDAALDESNVTRFCEILQRLAH
                     NTQFIVITHNRVTMTAAQVIYGVSMGSDSISRLLSLRLEEVPAGDR"
     gene            complement(10532..>11015)
                     /locus_tag="VGT44_04900"
     CDS             complement(10532..>11015)
                     /locus_tag="VGT44_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580171.1"
                     /translation="LGDTVSIGSLAEALSTSPDVVLMAIPGATMDATIAQYAAQLDGK
                     ILIDAANNMGASTPNSFAPLQQYTPRARIYRAFNIYGWENFANSEFEGGPPDLFYCGT
                     DGEARATVEQLISDIGLHPLYLGGVEQVGVVDSLLRLWFSLAVGQRKGRHLTFKVLTR
                     "
BASE COUNT         2555 a         2876 c         3409 g         2175 t
ORIGIN      
        1 cctgcatatt tcaaggagat ctcgtcgatg acagctctgg agcgcacctg tgctggctac
       61 ttagcatatc tgttctcaaa agagcgctca tgttggctct accggttctt tttctttgct
      121 atctactcat cagatagatg acgtgagggt ttcctgcatg aagtttactc tctgatagtg
      181 gaacggatat gctaagtagc cagcacaggc gtgaaaccct gtcaaacatc gagagatccc
      241 gctcgttcag gaagcacgtg aaccgcttga tgcgtatctc actcatcgtg aacatctggc
      301 ggaacagtgg cgtgagcgag ctgccgttcg aggagaaccc gttgcttcct ggagccgctg
      361 gcctgatggg catctctttc tcggtcaacg agggccactg gccgaacgtg gccttcggga
      421 aatcattgct aaactgggtc aggcagcaaa gctggcttcc cccctcggtc cacacgacct
      481 gcgtcatacg tttgccaaag cattgctcga tcctgctgcc tacggacttg accgaccccc
      541 gatgcctctt ccagctattc agcaactgct tggccatgct gatattgcaa cgacaaccat
      601 ctacacgcgg gtgtctgctg atgacctggc gcggatgatg gggacatctg aagcacccta
      661 aacgaaaact gtgccgcact tatctacttt gcggctacaa ggcctcttcc ttaacgcagg
      721 gccaactgga gagacccgta gggtgacgga gttcccgtag tactcaaatg atcgggtaat
      781 gcccgataca cggggaaggg gaactgggcg atgagaacgt ccgcgactgt gcggtggagg
      841 ccgcgaaccg caaggggata agcagtcaag gcagaccaag cctcaagcct ggagagtcgg
      901 atgcgtcgaa ggacgccagt cccgttctgg aaggggcggc tggaaaaggg tcgtccttat
      961 gaaacgacca gaaaggaatc ttggcatgtc tatggaaaac agcgacacct cgccgaccgc
     1021 ccacttcaca agaatgtgtt tccggcctgt ggacgcacca gtaacactgg acaggggcat
     1081 gatccagcac gaaacgggcg acgtggccga cggatttggg gccgatgata ggtttgtcgt
     1141 cgtagttagt gcggggacag atcaggatca gatcggcgtt ccactcggcg gcggcgttga
     1201 cgatctcgcg ttccgggcga ccagtgcggt ggatgcgttc tgcgccgggg aaggcgcgcc
     1261 cgccttcgac gaggataccc tgcgcggagg cgacttcagc ctggcgcatc tgttcacggc
     1321 gcgccggtgg caaacccgga ggccgcagaa agcgttcgcg ttggcgctct aagtcagctt
     1381 gcgggccggc atcggtgacg tagagaagtc caatggtgcg atcactcgtc gtaagcagcg
     1441 aagagactgc ttgacgtatt tgttcagtat tcgtcccgtc caaacagccg agtatgcgca
     1501 tacttccctc ctaaacaagc agaagccaga ttgctatggc tccgactatc agcatgaatg
     1561 gtgtcaccag gataccgact ttgaaatagt ccaaacctga tacatcaagg ttgcgttgac
     1621 gaaggattaa aagccacaaa accgtagcga gtgaaccaac ggttgtgagg tttggcccaa
     1681 gatcacaccc aaatatggta gcagcgatga aagcatgctg gatagcgatc ggggcgtgtg
     1741 gcaggccatg gagtgaagag gtcaggacaa cagccattgg cacattgtta atcaggttcg
     1801 ttccaaatgc tgctccgatc gttctggcga tgaccgcacc aaggctggta ccaccagaca
     1861 ggtgcaggag caattgacca aattgcgccg tgagtccggt gctttcgacc gtctggacaa
     1921 caataaacat gccggcaatg aaagcaaaga ttgatcagga aatctgtttg ccggtgttcc
     1981 acagcgtggt ctgtttccag aaaagcgcac cgaccagcag caagaatgca ccgccaagtg
     2041 cgacaagtga gagcgggagt tgtactgcgg cggcaaacac atatgccccg gcaacaaaaa
     2101 gtaacaccgc acacgcgtag cgaaagtacg ttttatgctt tattgactca tcaattggtg
     2161 gcaagcgctt catatcgaaa ctaccgcgga gctgcctgcg atagagaaag aggaacacgc
     2221 cagcattcat actaatcaca agcacgctcg gcaagaaaaa caaacgaagg aatgtgaata
     2281 agccgagggg gaattgcgag gtaacaataa tgttgatcgg attgctcacc ggtagtaaga
     2341 atgaggcact gtcagcgaca aacgtacagg caaaaagaaa cggcagcacc ggaagtcgaa
     2401 gccgggtgac gagactataa attattggcg tcaaaataag ggcggtggca tcgtttgaga
     2461 gaaacatcga gataaggctg ccgaggatga acgtgttcag aaaaaggcgc cgggaactgt
     2521 tccctgaaag tcgtgctgct tggaatgcca accaatcaaa cagacctgcc acttctgcta
     2581 atgctgagag gctcatcata ccaagaaaaa acagaaaggt attccaatct ttgagcagta
     2641 ccgccccggc atcgacggga ctgataaggc caagtagcag caagagtcca gcgccaacca
     2701 gcgctaccat tgcctcattc gattgcaatg gtcgagtcat gatacccacg agcgtcagta
     2761 ctgcaatgag tgaaataaga agcgttcttg tcagaacagg aagatgaaac aaaaagaaca
     2821 tgcgaaatct ctcctcgcct aacaacagga tgagtatgtt ctgctttata cacgggctta
     2881 accgtgagaa agagacagca ggacaaaaat gtttaagcct ggcagttccg tttattagat
     2941 caagacgagt aaaatgtcag gaaaagcact gtttgttccg gcgggagcat taggctgcca
     3001 taaatctagc aagcagcaac tctagccgtt tgagtagctc gtctaaatcg ctaaattgag
     3061 catttcgtct agacgatggg ggtcaggata agcgcggtgg cgtcgttcga gaggatcatc
     3121 gagatcaggc agcccagcag gaagatgttg aacagaagtc ggcgcgagct gccgctggag
     3181 agtcctgcgg ctttcgcagc aagccagtcg aagagtccag cggcgtcggc catggccgaa
     3241 agtgtcatca tgcccaggaa gaagaagaag atgttccagt cgctcaacag ggtggtcagt
     3301 gcatccagtg gcgcgatcag cccgagcagg agcaacaggg ccgcgccgcc cagcgcaatc
     3361 ctggcctcgt ccagcttgaa tgggcgaagc atgatgccga tgagcgtaca gacagcgata
     3421 aacgacacca tgatggtttt cgtgagcgcg ggtatctgga aaaaaagcag catcggatat
     3481 ctggcgtagt ctactaattc aaactcgaat acatgagtct atttgttaca cgcttcaagc
     3541 ggcgaaaaag tgacagcaag ggtggtagaa tttagcagtt accctcaaca tcgataacat
     3601 caggaggttt cctatggctc agcttggttt cgtaggtctt gggaacatgg gcggcggtat
     3661 agtcaaaagg ctgctggatg caggacatac cgttgtcggc tataatcgca cctggtcaaa
     3721 ggcgcaatgg ctgctggacg ccggtatgca gtggggcgag acgccgcgtc aagttgcgga
     3781 aacggcagat gtcaccttca gcatggtggc taattccgct gcgctgcgcg atgtatttgg
     3841 cggtcacgac ggcattctgg caggtttagg gccaggtaag atctatgtgg acatgagtac
     3901 cgtgagtcca agcatctcac gagaactgtc tgaacgtgtc gccgcgaagg gagcgcagat
     3961 gctcgatgcg cccatatcgg ggaacgtcat taccctggta cagggaactt cgtccatcat
     4021 ggtgggcggc gaccaggcga cctttgagaa gatcaaacct attttgctgg atatcgggtc
     4081 aaaagtgaac tatattggcg ggaacggcca ggcgctttta atgaaggccg caatcaacct
     4141 gagcctgccg atacaattca tgggtttctg cgaaggagtg ctgctggccg agaaaggcgg
     4201 tatccctcgc gaaaccgcca tcagggtatg gttgagcagc gcgcttgtgt cccctgccac
     4261 ggagcatcat gcgttgctct tgcaggagaa gtcggaagag gttatgttta atgtgaacat
     4321 gatgcagaag gatatgctgc tggcgttgga actggggaga gaactcgacg tagcgctacc
     4381 agccgtcgcc ctttctaacg agatactcac ggctgctcga gcaatgggtc tcgccagaga
     4441 agactatgcc atcatgttcg aggtactggc acaactggcc gggttgaata acgaaagcag
     4501 cgcgaaagga gaccaggatg catcacgtta gaaattatag caatgcgacc tttcaggtgc
     4561 ctcccggtta tgagaagcac agtttggatt actcccgtgt cgcattagtc gatcacacga
     4621 ccggttcagt tcacatggga ctgggtctgt gtaagctaga gccggaaggg actttggaac
     4681 ctcacgttca cttctatgaa gaggctttct atatcctgga aggacaggtg ctgggaagca
     4741 tcgatggtcg ggtctatcgc tttggtccag gcgactatgg cattattcaa gccgccgtga
     4801 cacatgcctg gcggggtgtt ggagatcagc ccgctcgctg gctggagatg ctgtcagcgc
     4861 agcctaaacc tcccggcgga gaacgcgata cctttttctt gaagggagcc gccgcgccgg
     4921 acgaaggcaa tccgcccgac ctgcgcaacc cgctgacccg ctatcttggt cacttcgacg
     4981 atcagcacat gccaccgccc agccagttgc agatggatgg ctaccggggc agcaatatct
     5041 atggtatctc cttgaagatg ctcgttgatc gcatgttggg ggcgcagcac ctgacgctgt
     5101 tcatggtcga gttccagccg ggtggcgatg gtaacgtgca tgaccacccg tttgaggagt
     5161 cgtactttct tgttagcggc gaagctgaca ccatcctgga tggcaagcct tatcatgtga
     5221 aggcaggaga ttatgtctgg acgggtgtcg gcagtacgca cggattcttt aacaggggta
     5281 ccgttccggt gcgttggata gaaacacagt caccgcagcc gccaaaccag caagcgttcc
     5341 ggtttccgag tgattgggca tacctggccg ccaggctgga agattccgag tgattgtctc
     5401 gacatctcgt cggattcata ggcatagaaa atctgaggct tgcagggcga tcattacggt
     5461 cgccctgcaa gctctgcatt cccaaccgac tcgtgggcta agacaatgct gaggccgtct
     5521 taacccgctt cctcggcgtc ccaatcccat tgtccctctt gttcggccaa caacgcctcc
     5581 actcgctcgg ctttcgcttc gcggctggtg cgcggctttt gcgatggtaa cgagaccacc
     5641 agctcgaagt cgtcgtcggc ggcaatgcgg ttgaagcgtt ccacttcgca gccaaagcag
     5701 gagtgaacaa ttacgtactc gccatttggc cgttgaaagt agccgaccgg ctccatgagc
     5761 gaaccacatg gactcattcg atctcccggt ttcttgtcat cgacatgacg agaatataaa
     5821 caaaacgggc agtgattgcg atggcggccg ccgctcggcg gcgggcaaac gaatcgacgg
     5881 cagcgcttgc acttgaacac ctcatccgat gatcgattat accggggctt acgacgctct
     5941 ttccgttcat cgtcacccca atcatcctcg aattggtcct gttggttgcg ggaatatctt
     6001 tgctcttcta aacgcagcaa tttcctgcgt ggtgtatgca tcggtgatcc tcttctttat
     6061 tttcgctcgg ttgtttacca gtagttggtc tggctgtccg gcggcaaaag caacttttgc
     6121 ttgccagccg gtaggggcaa gcgcaagcac cgatgctgaa ggtatggtcg tgtttaacat
     6181 aaagcctccg ctctttctaa ggctcagtgg tcttcaagac atgcgccgac tgaattgcac
     6241 gtgggctggc cacgtgcctt gcctgagtga tgtgattact tctggacctg gcgctatcta
     6301 tgctggcaca aagcatacag ggcagctttg cgcgtgtcgg gcggacaaat gcccgggcta
     6361 cagcgccaat gtcaaacggg tatggtggta atgttgatta ttgttggtgt ggttgcgaag
     6421 cgcgaggagg gcgaagctga cgaggagagt aaatgtgtac ttgcagaggc aagcacaacg
     6481 gaactctgag aactactcag tgtagcgtat tacgaatgtc gattgtgttg agtagtattg
     6541 cgtcagcagc ccaccgcgtt catgggtgag aggacttgca ttaagaagaa cctttctaga
     6601 tttgtgtagt gttaactgaa ctattataga atatagccgc ctttgcgaga aaagtcaaga
     6661 aaaacctcga cggatttccg gcgctatggt atcatctacc cgcaatcatc tgtgtttacc
     6721 gaaggacgaa tgatgctgtg tatctgaaac gcctggaaat gttggggttc aagagttttg
     6781 ccccgcgcac gatgctggaa tttagcacgg gggtgacggc cattgtaggt ccgaatggcg
     6841 cggggaagtc gaacgtcgcg gatgcggtgc gctgggtgct gggcgagcag agtatgcgcc
     6901 agctgcgcgg gaagaggggc gatgatatta ttttcgcggg cgggttcggc aaagctgcgc
     6961 tgggcatggc cgaggtgtcg ctgacgttgg acaacagcac aggctgggtg ccttcagaat
     7021 acagcgaact gaccgtgacg cgccgcagtt atcgctcagg cgaaaacgaa tacctcatca
     7081 acaaacagaa agtgcgcctc aaggatgttg tcctgctgct cgcgcaggcg cgtatcgggc
     7141 atgattcata caccgtcgtc gggcaggggc tgatcgatgc cgcgttgagt ttgcgcgcgg
     7201 aagagcggcg cgggctgttc gaggacgccg ctggtattcg cccctaccag gtacagcgcg
     7261 ccgattcgga gagccgcctg cgccagacgg agagcaacct ggaacgcctg cgcgatatcg
     7321 tcaatgagat cgagccgcgc ctggaacccc tggcggagca ggcgcgccgc gcaactgaac
     7381 acagacagtt gaatagcgag ttgcaggatg tcctgctgac ctggtatgcc ttgcagtggc
     7441 gtcgcctgcg cgcaacccag gagcgggtgg aggttgagga gcaggcgatg tccgggcgcg
     7501 tgcagcatgg tgagcgagaa ttgcagactt tcgacgagcg ccgcgataca ctgcgacaga
     7561 gcaggcagga ggcgcaaaat gcaattgccc aggcgcgcga ggcgtctaat cagtgcctcg
     7621 cgagcgcgca gcatatcgag cgcgagatgg cggtcggcca ggagcgcatc gtgggcttgc
     7681 agcgccagcg cgacgaacag cagcaggagg agcgccgcct gcgcgagcgg ttgatcacgg
     7741 cccggcaaca gttgatcgac ctggaagaac agtgcgaact ggcagaggag ggcattgata
     7801 cggacgccgc cgcgctggcc accttcgagg ggcaggtagc gcaggcccaa aaagcctacg
     7861 agatggatga gcgccgcctg cgcaacgcgc aaagcgagct gatccaggtg caggcgcgcc
     7921 tgggggccag ccagtccgac ctgggcaggc agcaaaagca ccttggcgag cgtaaccgcg
     7981 cgctggcagc tcgccgcgag accatcgcgc aatcgcaaca ggccacgcgc gcgcaggaaa
     8041 cgcgcctggt cgaagaacgc gcccgcctcg acaccgcgcg ccaggaagag cagcagatca
     8101 cacagcgcaa gcaaagcatc gggcagcaga tcggcgacgc ccagcaggag atcgagcgct
     8161 tcaagcaggc gttgaacgaa gccgaacggc agcgacgtat cgtggccgac cggctcaaca
     8221 tgctcaagaa ctggcgcagt agcctgagcg ggtatagcga cggtgtgcgg gccatcttac
     8281 gcgcgccctc cggcaaactt ggcggcctgc ttggtcccat tccgcaactg ggctatgctc
     8341 ccgccgggct agaaacggcg cttgaggccg cgctcggctc ctacctgcag gcggtcgtgg
     8401 tacagtcgct cgaccaggcg caagcctgcc tgggtttctt gcaggcgacg aacgccggaa
     8461 aagctatgat tgcctggctg gatgatgaca cataccaggc gaatattggg aattatcggc
     8521 aagaagtgga gacgctgaaa cgcttgcttg tggatatgcc gggaatgcag gcgcgggtgc
     8581 gagggctggc ctggcagttg atgcaatgcg aaccgcgcta tctcccgctg ttccagcgta
     8641 tcctgcgcgg cgcggtcgtc acgcaagacc tggagacggc gcgcgctctg ctggatcgcg
     8701 tcgtgcgaat gccggcgaac gagcgtcacc tggaaacgtt tgtgacgctg cgcggtgaag
     8761 tgctgcacgt ggatggatgg ctgacgggcg ggagtggcaa ggacggcggg cagcagggtg
     8821 tactggcata cgaacgcgaa ttgcgcgaat tgccggagca gctcgaccgg cacgtggcgt
     8881 tgattaacga gttgaacggc aatatcagcg aggctcaacg cgcgcaggaa gcgcggcgcg
     8941 ttgagcagag cgccatcgag aaagagttgc agcgcaacgc cagccgtgtc aatgagatca
     9001 gtaagggggt ggccacgacg cagcgcgagc ttgatcgctt gcagtccgaa ctgcaactcg
     9061 cgcgctcggt cgagcagcaa ctggcgtctg agattgctgg gctggatcag gaggtgcagg
     9121 ccgccgtaga gcgggtgcgc gcgcacgaga agtcgcagcg cgagatgacc ggcctggttg
     9181 aagaactgca gcgcgaggtc gaggagcgca cactggtgtt tcgacgccag caggacgacc
     9241 tgggcaaggc gcgcaccgcc ctggccgtca agcgacagga ggcgaaggcg ctgcgccaac
     9301 aactggttgc ccagcaggct gctgcgcaag acttgaaggc gcaaatcgat cagcgtgtgg
     9361 cgcgagcgaa ggaggtggag acgcaattgc aggcgttgca gacggcgctt gaaggacagc
     9421 gcgccaggtt ggaaaaaaac cggacgcagt cacagcagtt gttggaggcg ctgcaaaacg
     9481 ccgaagctcg ggcgggtgag ttcgatacac agctcctcga gctggaacag gagacgctgc
     9541 gagcgcgcca ggcgctcaac gagttggagg ttggctatcg tcaatgcctg ctggcgagtc
     9601 agaaggcgcg tgatgccgtt gccgccttgc ttgagcaatt gcaggaagag gtgggcatcg
     9661 ccgatcctgc tgaactggtg cgttatgcgg cgcgagcgaa tgtgacactc gtggacgagg
     9721 cgggcggcga agaggctcga cctgcgcagg aggagagtgt ggtgctgtcg gaagaggagg
     9781 aggcgcagct gcgcagattg cgcaggcggg tcgatacgct gcgcagccgc ttgcgagcgc
     9841 tgggcggctt tgatcccgac gccccgcagc agtatgaaga gacgcgcacg cgctacgaat
     9901 ttctcagtac gcagatagcc gatatggaac aggcagcgct gcaattgcat tcgatcatcg
     9961 accagcttga tgtgacgatg gcgcgccagt tcgagatcac atttcagacc gtgaacgcgc
    10021 gtttcgccga acacttcacc actctgttca atggcgggca ggcgcggctt gaattgacgg
    10081 ttgcgaagca ggaagatgaa gacgggacgc tgcgcgcgaa ccccgccgga atgcccgccg
    10141 gggtagaggt gattgtgcag ccgccaggga agaaggtgat ggacctctcg ctgctctccg
    10201 gcggcgagcg ggcgctggtc tcggcggccc tcttgttcgc gctgttggag atcaacccgc
    10261 cgccattctg cctgctcgat gaagtcgatg cagcgctgga cgagtcgaat gtgacacgct
    10321 tctgcgaaat tttgcaacga ctggcgcaca acacgcagtt catcgtcatc acgcacaacc
    10381 gcgtcaccat gacggcggca caggtcatct acggcgtctc gatgggcagc gacagcatct
    10441 cgcgcctgct gtcgctgagg ctggaagagg tgccggcggg agaccgctag ccgaaaagat
    10501 cggggtctta cgttgggcgg tcgtagagga tttagcgggt gagtactttg aacgtcagat
    10561 gtcgcccttt gcgctgacca acagccagcg aaaaccacaa tcttaaaagc gagtccacca
    10621 cgccgacttg ctcgactcca cccaggtaca aaggatgcag tccaatgtcc gaaatcaact
    10681 gctcgaccgt cgcgcgcgct tcgccgtcgg ttccgcagta gaacaggtct ggcggtcctc
    10741 cctcaaattc cgaattggcg aagttttccc acccgtagat attgaaggcg cggtagatgc
    10801 gagcacgtgg tgtatattgc tgaagtgggg caaagctatt cggcgtgctg gctcccatat
    10861 tattggcggc atcgatgaga atcttgccat ccagttgagc ggcatactgt gcaatggtgg
    10921 catccatggt tgcgccaggt atggccatca gtaccacatc tggactggtg gagagagctt
    10981 cggctaacga tccgattgat actgtgtctc ccaac
//
LOCUS       DASZAK010000282         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_504485, whole genome shotgun sequence.
ACCESSION   DASZAK010000282 DASZAK010000000
VERSION     DASZAK010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504485"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VGT44_04905"
     CDS             <1..306
                     /locus_tag="VGT44_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580172.1"
                     /translation="EGDTSSLAILDRFQSQPPDPATLVSTFSQAKANLSWKSINVLGY
                     RGEADTTVDSYIEVDLSTNGPGGNVNAFAFFHFVTATLQTGPGIIGINVEPLRASQT"
     gene            381..2360
                     /locus_tag="VGT44_04910"
     CDS             381..2360
                     /locus_tag="VGT44_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2580173.1"
                     /translation="MAEKLNHAERLDKSIDQGMDQFGRFAKRRWLWLVLFSIILWYFV
                     TQIGPQLSPPSPGTVFQYTFQIFFYMIAIMFQFVALFWFLGRPRLYWVMPGETGVGFE
                     DYKGNPEVLESARRIVMLLRGIEEYDKMGGLPVRGLLLSGPPGTGKSYLAQCISTETG
                     VPFAYASAASFRAMFIGMDVLMIKRLYNKARRYAREYGGCVIFMDEIDAIGMSRSARG
                     GGQMAMGGGIFGAMGGTGGLNELLMQMDPPNIETGWFKKILRVLGLYHSRAQAQPVLT
                     IGATNIPESLDPALLRPGRFDRKIHIAPPTDKYRSEVIEYYLEKISHDPSISLAALVA
                     RFVEYTPVAIKHVINEAVIIAHFDGRDMVTYKDLIEAQDVHEFGLRQPSELTAIERRR
                     LAYHEAGHAVAAYYLLEREFPAYVTVHRRGDLEGAEAFAAFRPKETIKTHAKEEILAH
                     MQIALASRAAEELFLDINLSGVAGDFASATAAARAYVGIYGMDGTISSFLGYSRYTGQ
                     AQPRAPEDISKRAEAVLQSQFKAVKRLLQDHREALIAVAEALIERDELVADEIKRVID
                     EADAHRVTRLVMSEFEEVLGSSSNGYSNGTNGTNGKNGHALVGVGSNGDGRDGDEPDM
                     IDSTLVIPDSQAGYGNVQGFADPLSNDDGSLYMGG"
BASE COUNT          516 a          698 c          740 g          500 t
ORIGIN      
        1 gaaggcgaca ccagttccct tgccatcctt gatcggtttc aatcgcagcc gcctgatccg
       61 gcgacgcttg tcagtacctt tagccaggcc aaagcaaact tgtcgtggaa aagcatcaat
      121 gtgctgggat atcgcggcga agccgatacg accgtcgata gctacattga ggtagacctt
      181 tcaacgaacg ggcctggtgg caacgtcaac gcctttgcat tttttcactt cgtgactgcg
      241 acgttgcaga cgggtcccgg tatcatcggt atcaacgttg aaccattgcg cgcatctcaa
      301 acgtagttgc gtttcaatag agcgttattg cgacggtaag ggggcctggg agggcatccc
      361 caaacaaaag gaacacggtt atggcagaaa aactcaatca cgcggagagg ctggacaaat
      421 caatcgacca gggcatggat cagttcgggc gatttgcgaa aaggcgctgg ctctggctcg
      481 ttctcttcag catcatcctg tggtattttg tgacgcagat tggcccccag cttagcccgc
      541 cttcgccggg gacggtcttc caatatacgt tccagatctt cttctacatg atcgcgatca
      601 tgttccagtt cgtggcgctc ttctggttcc tgggtcgccc gcgcctgtac tgggtgatgc
      661 cgggggagac gggcgtcggc ttcgaggact acaagggcaa ccctgaagtg ctggagtcgg
      721 cgcggcgtat agtcatgttg ctgcgcggca tcgaagagta cgataagatg ggcggcttgc
      781 cggtgcgcgg cctgctgctc tcaggccctc cgggaaccgg caaaagctac ctggcgcagt
      841 gcatcagcac agaaaccggc gttcccttcg cctatgccag tgccgccagc ttccgcgcta
      901 tgttcatcgg catggatgtg ctgatgatta agcgcctcta caataaggcc cgtcggtacg
      961 ctcgtgaata cggtggctgc gtgatcttca tggacgaaat cgatgccatc ggcatgtcgc
     1021 gttccgcgcg cggcggtggt cagatggcga tgggcggcgg catctttggc gcgatggggg
     1081 gaaccggcgg cctcaatgag ctgctgatgc agatggaccc gcccaacatc gagacaggct
     1141 ggttcaagaa aatcctgcgc gtgctgggcc tctatcatag ccgcgcgcaa gctcagccgg
     1201 tgctgaccat cggcgcgacc aacattcctg aatcgctcga tcccgcgctg ttgcgtcctg
     1261 gccgtttcga ccgcaagatt cacatcgcgc cgccgaccga taaatatcga agcgaggtga
     1321 tcgagtacta cctggaaaag atcagtcacg atcccagcat ctcgctcgcc gccctggtcg
     1381 cgcgttttgt cgaatatacc ccggtcgcca tcaaacacgt catcaacgaa gcggtcatca
     1441 tcgcgcactt tgatggtcgc gacatggtga cgtataaaga cctgatcgag gcgcaggacg
     1501 ttcacgagtt tggcctacgg cagccgagcg agctgaccgc tatcgagcgc cgccgcctgg
     1561 cctatcacga ggccggccat gccgtggccg cttactacct gctcgaacgt gaattccctg
     1621 cttacgtcac cgtccaccgg cgcggcgacc tcgaaggcgc ggaagccttc gccgcttttc
     1681 gtccaaaaga aaccatcaaa actcacgcta aggaagaaat ccttgcgcac atgcagatcg
     1741 cgctggcatc gcgcgccgct gaagaattgt tcctcgatat taacctgagc ggcgtcgcgg
     1801 gcgacttcgc cagtgcaacg gcagctgcga gagcttatgt cggcatctac ggtatggatg
     1861 ggacgatctc atcgttcctc ggttattcca ggtacaccgg tcaggctcaa ccaagagcgc
     1921 cagaggacat ttccaagcgc gctgaggccg ttctccaatc acagttcaag gctgtcaagc
     1981 gccttttgca ggatcatcgc gaagccctga tcgctgtcgc cgaggctttg atcgagcgcg
     2041 acgaactggt ggccgacgag atcaagcgag tcatcgacga ggccgacgcc caccgcgtga
     2101 cgcgcctggt catgtccgaa ttcgaggagg tgcttgggag cagcagcaac ggctactcaa
     2161 atggaacaaa cggaacaaac ggcaagaatg gccatgcgct ggtcggcgtg ggaagtaacg
     2221 gcgatggccg cgacggtgac gagccagaca tgatcgattc gacgctggtt atccctgata
     2281 gccaggctgg ctatgggaat gtgcagggct ttgccgaccc gctgtccaac gatgatggat
     2341 cgttgtatat gggtgggtag atcgagtaaa gatgcgtagg gacagggacg cggggagagg
     2401 agtggcgggg gccttgcgcc tgtccgggtg gcaaacgaat cgtgacctcc accg
//
LOCUS       DASZAK010000283         5519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_397836, whole genome shotgun sequence.
ACCESSION   DASZAK010000283 DASZAK010000000
VERSION     DASZAK010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397836"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..589
                     /locus_tag="VGT44_04915"
     CDS             305..589
                     /locus_tag="VGT44_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580174.1"
                     /translation="MHRTREELEGHIVPLADDMDEFNAGVIAQYQNWTRSAIEEKFAA
                     TLATFERFLHDLPETALENERILLWLRIDAIDHYEDHRLPDAPHIVVMYN"
     gene            594..1313
                     /locus_tag="VGT44_04920"
     CDS             594..1313
                     /locus_tag="VGT44_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEV2580175.1"
                     /translation="MNISRFYLPDAHQAQGIVIVIDVIRAFTVAAYAFAGGASRLWLV
                     RTTDEAFALRKREPTALLAGEIGGRLIPGFDLNNSPSLMAASDVRGRTIIQRTGAGTQ
                     GAAGAVNATRLLICSLVNARATAQYARELAATLCEPITLLPTEEGFPASLKHRAGEDT
                     ACADYLEALIRQQDNASALLAENLARLEARGRFAFFTPGDSDIPIEDVALIKAVDRFD
                     FVMAGTHQQWEGIAYVEVERH"
     gene            complement(1466..2647)
                     /locus_tag="VGT44_04925"
     CDS             complement(1466..2647)
                     /locus_tag="VGT44_04925"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEV2580176.1"
                     /translation="MSEQVPVISAAVRTPTGKFLGGLAGFSAPQLGAMVIKEAVKRAG
                     IEPEAIDEVIMGNVVSAGIGQAPARQAAIGAGLPVDIPAFTVNKVCGSGLKAVMLAAQ
                     AIRAGDAAAFVAGGMESMSNAPYLLPKARTGYRMGNGEIVDAVVHDGLWCAFEDVHMG
                     NEAEIIADKFCVTRDEQDHFSLQSHQRAAAATAAGRFKDEIVPVEIKQKGGVVVLDKD
                     EPIRADTSLEALTKLKPAFREGGTVTAGNAPGLSDGASATVVVSQEFATGHSLPVLAR
                     ITGYASAAIEPRYIFAAPTRAVRRLLERTGLKIGDFDLIEVNEAFAAQALANGKELDW
                     DWSLTNVNGGAVALGHPIGSSGSRVLTTLIHELRRRGGGRGLATLCLGGGGAVAMSIE
                     V"
     gene            complement(2751..3836)
                     /locus_tag="VGT44_04930"
     CDS             complement(2751..3836)
                     /locus_tag="VGT44_04930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580177.1"
                     /translation="MNNTSDENYTNNTPKANSANGSQNMNHRGLPPIPAVGESGPDVC
                     AIVQMYLAIIDDLTPQEVALLSEHVVTCAACGEAFELMERATHLVGSLTASAPSPRVD
                     VAMLALTTAGTGTRQVRQAGRQPVVANSVTARRSQRPARRRKVAWVVSELVAAAVILL
                     ALFASLHQFGIFGGGPQTAFALPSSLSWSGFVLYHSETRTGTHGELYEVESYHDLKTG
                     SVHVETKMDGQLDVVTVSDGQQVLGMDTIHHIAQMGANQWTVNDSMFNLNELRRELQM
                     GQATYLGLDSFKGQSVYRIRCTNGLVMLLDMQYMPVNVLRGAVGPGTGSPLYTNLELL
                     TSTQVPDSMWNMSVPPGYQMGTLPAQP"
     gene            complement(3833..4516)
                     /locus_tag="VGT44_04935"
     CDS             complement(3833..4516)
                     /locus_tag="VGT44_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2580178.1"
                     /translation="MAAPTGGQLPQRTESARAGMDRKKIGESQYLEVVSSGEDAAILD
                     FGTLYEKFKRPIHAYIYRMLNSQEDADDITQEVFTRAYLAWNDLYDRGNLSPWLYRIA
                     TNLCVDMLRRRKRISWWPLSRAGWGDDVGEYRRDAEAAYLPSDSGGIPEVGEREHIQK
                     TLARMPHEYAVALVLSAAQGISYQEIAVIAGISPNAAATRISRAKRIFAELYQRANEE
                     GVGIQERKR"
     gene            complement(4672..5220)
                     /locus_tag="VGT44_04940"
     CDS             complement(4672..5220)
                     /locus_tag="VGT44_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580179.1"
                     /translation="MSSQPSIDPVIQQLQLLLTGYGYNFYNTTNQARADDLLVRERAS
                     YHLGQAIDMLARLQGEYQRRFIPPLTRANPDPPAEALAQAREIEATRQALSDIETHIR
                     GMSVPTQDRTWWRFRQEQTLLGQLLNFDLALVRGCEEVYQYVAQLTPEGWNSQGSAAL
                     RQLMQRVSNIARERERFLLIPM"
     gene            complement(5234..>5519)
                     /locus_tag="VGT44_04945"
     CDS             complement(5234..>5519)
                     /locus_tag="VGT44_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580180.1"
                     /translation="LAIYGISLDTVKVLVFPRDERMKALISLKAFGLSEMEAVRYYTA
                     MLMAERGVVSAPNMAIGQPFTIGGVQPTGSVDRYVNNAAPATLPAPTPSR"
BASE COUNT         1133 a         1796 c         1472 g         1118 t
ORIGIN      
        1 aaaagcactg gcggttgtac caggattgtg ctgtttgttc atacagcagg atgatttcgt
       61 tcggtcgtgt aacataataa aatgtgtagt acttcgtcaa aatgtcattg gagcaaatac
      121 atgccatcac aagaacatca attcagccgc caggaacttt taatcgccct tgagcagggc
      181 tggaagcact acctcccact tctggccgca ctgccggagg aagaacaggc ccgttacgcg
      241 caggagcagg gtttctctcg ttcaggatgt ggtagcgcat atttgcgcgt ggtgggagct
      301 atcaatgcat cgcacacgcg aggaactgga aggccatata gttcctcttg ccgatgatat
      361 ggatgagttc aatgctgggg taatcgcaca atatcagaac tggacccgtt cagccatcga
      421 ggaaaaattc gcggcaacgc ttgcgacttt cgagcgcttt ttacatgatt tgccggaaac
      481 agcgcttgag aatgagcgca tccttctctg gctgcgcatt gatgccattg atcattatga
      541 ggaccatcga ttgcctgacg cgccgcatat tgttgtaatg tacaattagc aacatgaaca
      601 tatcccgctt ctaccttccc gacgcccacc aagcccaggg catcgtcatc gtcatcgacg
      661 tgatccgcgc cttcaccgtc gccgcctacg ccttcgccgg cggcgcatcg cggctctggc
      721 tggtgcgtac caccgacgaa gccttcgccc tgcgcaagcg cgagccaacg gcgctgctgg
      781 ccggcgaaat cggcggtcgt ctgatccccg gcttcgacct caacaactcc ccctcactca
      841 tggccgcctc ggatgtacgc gggcgtacca tcatccagcg caccggcgcg gggacgcagg
      901 gcgcggcagg cgcagtcaac gccacccgcc tgctcatctg ctcgctcgtc aacgcgcgcg
      961 ccaccgcgca gtacgcccgt gaactggccg ctacattgtg cgagcctatc acactcctgc
     1021 cgaccgaaga aggcttccca gcatcgctca aacatcgtgc cggtgaggat accgcatgtg
     1081 ccgattatct tgaagccttg atacgccagc aggataacgc ctcagcgctc ctcgccgaaa
     1141 atcttgcgcg attagaagcg cgcggtcgtt tcgcgttttt cacacccgga gatagcgaca
     1201 tccccatcga agatgttgcc ctgattaaag ccgttgaccg cttcgacttc gtcatggcag
     1261 ggacgcatca gcaatgggaa gggatcgcgt atgtagaagt agagcgacac tagcgcggag
     1321 caagggtttc cgagggcgac cacaagggtg cgcccttacc atgactgcga aacggcgctt
     1381 gcgttgccat ccaactggtc atggtaaggg cgcacccttg tggtcgccct cggaaacctc
     1441 ttccatgcaa aaaatttagc gcgcgctata cttcaatgct catcgcgacc gcgccgccgc
     1501 cgcccagaca aagcgtagcc agcccgcgac cgccgccgcg cctgcgcagt tcgtggatca
     1561 gcgtcgtcag cacgcgcgag ccgctggagc cgatggggtg gcccagcgct accgcgccgc
     1621 cattcacgtt ggtaagcgac cagtcccaat ccagctcttt gccgttggcg agcgcctgcg
     1681 ctgcgaacgc ttcgttcacc tcgatcaggt cgaaatcgcc gatcttcagg ccggtgcgtt
     1741 cgagcaggcg gcgcaccgcg cgcgtgggag ccgcgaagat gtagcgcggc tcgatagcgg
     1801 ctgaagcata gccggtgatg cgcgccagca ccggaaggct atgtcccgtc gcaaactcct
     1861 ggctcacaac aacggtggct gacgcgccat cgctcaatcc tggagcgttg ccagccgtga
     1921 ccgtgccgcc ctcccggaaa gcaggtttga gcttcgtcag cgcttccagc gacgtatcag
     1981 cgcggatagg ttcatcttta tcaagaacca ccacgccgcc tttctgcttg atctcgaccg
     2041 gcacaatctc atctttgaaa cgcccggcag ccgtcgccgc agccgcgcgc tggtggcttt
     2101 gcagcgagaa gtgatcctgc tcgtcgcgtg tcacacagaa cttgtcggca ataatctccg
     2161 cctcgttgcc catatgcaca tcctcgaagg cgcaccacag cccgtcatgc acaaccgcgt
     2221 ccactatctc gccgttgccc atgcgataac ccgtgcgcgc tttcggcagc aggtaaggcg
     2281 cattgctcat actctccatg ccgcccgcca caaatgccgc tgcatccccg gcgcgaatag
     2341 cctgcgccgc cagcatcacc gccttcaggc cggagccgca caccttatta accgtaaaag
     2401 cggggatatc aaccggcaac ccggcaccga tagccgcctg gcgcgcgggc gcctgcccaa
     2461 tgccagccga cacgacattg cccataatca cttcatctat cgcctccggc tcaataccgg
     2521 cacgtttcac cgcttccttt ataaccatgg ctccaagttg tggcgcgcta aaaccggcca
     2581 gcccgcccag aaacttgccg gtcggcgtgc gcaccgccgc gctaataaca ggtacctgct
     2641 cagacataca aacatgctcc tttgaggccc cactccgcct ccgttccaca acatcgtggg
     2701 tctgaatatg tatcgtgaat cacccgtaat attgtatcac agggaaaatg ctacggctgc
     2761 gctggcagcg tccccatctg gtatcccggc ggcacgctca tattccacat ggaatcaggc
     2821 acctgcgtcg aggtcagcag ttcgaggttc gtgtaaaggg gagagccggt gccggggcca
     2881 accgcgccgc gcagcacgtt caccggcata tactgcatat cgagcagcat cacgaggccg
     2941 ttcgtacagc gaatgcgata cacagactgg cccttgaaac tatccagtcc caggtaggtc
     3001 gcctgcccca tctgcaactc gcggcgcagc tcgttcagat tgaacatcga atcgttaacg
     3061 gtccactgat ttgcgcccat ctgagcaata tgatgaatcg tatccatacc gagtacttgc
     3121 tgcccatcgc ttaccgtgac aacatcaagt tgcccatcca tcttcgtttc gacatgcacg
     3181 ctgcccgtct taagatcgtg gtagctctct acctcgtaga gttcgccatg cgtcccggtt
     3241 ctggtttcgc tgtggtacag cacgaagccg ctccaggaaa gcgaagacgg cagcgcaaac
     3301 gccgtttgcg gcccgccacc gaagatgccg aactggtgca gcgaagcgaa caacgccagc
     3361 agtatgaccg ccgccgcgac cagctcgctc accacccagg ccaccttgcg gcgtcgcgcc
     3421 gggcgctggc ttcgtcgcgc tgtaacgcta ttcgccacca ccggctgccg tcctgcctgc
     3481 cgaacctgcc gtgtccctgt tccagccgta gtcaaagcca gcatggccac gtcaacacgg
     3541 ggcgatggcg cgctggccgt caggcttcct accaggtgag tggcgcgttc catcaactcg
     3601 aacgcctcgc cacacgccgc gcaggtcaca acatgctcag agagcagcgc aacttcctgt
     3661 ggcgtcaagt catcaatgat agccaggtac atctgcacaa tggcgcagac atccgggccg
     3721 ctctcgccca ctgctggaat tggcggaagt ccccgatggt tcatattttg agaaccattc
     3781 gcgctattgg cttttggcgt gttattcgta tagttctcat cgctggtgtt gttcatctct
     3841 tccgctcctg tataccaaca ccttcttcat tggcacgctg gtagagttca gcaaagatcc
     3901 gcttcgcgcg tgaaatgcgc gtggcggcag cgtttggaga aatacccgcg atcaccgcaa
     3961 tttcctggta cgatatccct tgcgccgcgc tcagcaccag cgccacggca tactcgtgtg
     4021 gcattcgagc aagcgtcttc tggatatgtt cccgctcgcc gacttcgggg atgcccccgc
     4081 tatccgaggg cagatacgcc gcctcagcat cccggcgata ctcgcctacg tcatcacccc
     4141 acccggcgcg cgagagcggc caccaggaaa tgcgcttgcg cctccgcaac atatcaacgc
     4201 acagattggt cgcaatgcga tacaaccagg gtgacagatt tccccggtcg tacaggtcat
     4261 tccatgccag gtatgcgcgc gtgaatacct cctgcgtgat atcgtccgcg tcctcctggc
     4321 tgttcagcat acgatagata taagcgtgaa tcggacgctt gaacttctca tacagcgtcc
     4381 caaaatccag aatagcggca tcctcacccg aagataccac ttctagatac tgcgattcac
     4441 ctatcttttt tctgtccata ccggcacgtg cgctctccgt tcgctgggga agctgcccac
     4501 cagtcggcgc agccatctcc ttgatatccc cagcaggcca tgctgctagt ataccaggaa
     4561 tagcaacggg agcacacgcg ccagtctcaa ttgatcctgc catatatccg tgaatgtcga
     4621 agcccttaca tacgatttcc ttctactata cacgctggca ggggttgagt tttacatcgg
     4681 tatcagcaaa aaccgttccc gttcgcgcgc aatgttagag acgcgctgca tcaactggcg
     4741 cagcgcagcg ctcccctggc tattccagcc ctccggcgtc aactgcgcca cgtactggta
     4801 cacctcttca cagccacgca ccagcgccag gtcaaagttg agcagttgac caagcagcgt
     4861 ctgctcctgg cggaaacgcc accacgtgcg atcctgcgtc ggcaccgaca tgccgcgaat
     4921 gtgggtctcg atatctgaga gcgcctgtcg cgtcgcctcg atctcgcgtg cctgcgccag
     4981 cgcctccgcg ggcggatcgg gattggcgcg cgtcagcggc ggaatgaaac ggcgctggta
     5041 ctcaccctgc aagcgcgcaa gcatgtcgat ggcctgcccc aggtgatagc tggcccgttc
     5101 ccgcaccagc agatcatcgg cccgcgcctg gttcgtcgtg ttgtagaagt tgtagccgta
     5161 ccctgtcagc agcagttgca actgctggat gacgggatca atcgatggtt gggaactcat
     5221 agcgcttcct ctattacctg ctgggcgtag gtgccgggag cgtcgccggc gcagcgttat
     5281 tcacatagcg atcaacgctg ccggtcggct ggacaccgcc aatagtgaag ggctgcccaa
     5341 tcgccatatt cggtgccgaa accacgcccc gctcggccat cagcatcgcg gtatagtagc
     5401 gcaccgcctc catttcgctc aggccaaacg ctttcagcga gatcaacgcc ttcatgcgct
     5461 cgtcacgcgg gaagaccagc accttgaccg tatccagcga gatgccgtag atggccaga
//
LOCUS       DASZAK010000284        10713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_362663, whole genome shotgun sequence.
ACCESSION   DASZAK010000284 DASZAK010000000
VERSION     DASZAK010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 10713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10713
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362663"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGT44_04950"
     CDS             <1..214
                     /locus_tag="VGT44_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580181.1"
                     /translation="NGGKPLSAQFIAERLSLPLAQVIAILDDLQQHLVFLVQQPAGEV
                     RWAFPVTVEPTPHRLRFSSGERIYAA"
     gene            242..439
                     /locus_tag="VGT44_04955"
     CDS             242..439
                     /locus_tag="VGT44_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580182.1"
                     /translation="MQGHLGHENLSVEIATACAHCGQPIHIGLDSALRWSIQEPDAAP
                     LVFMPDVDWGRFTEPTILDAY"
     gene            749..1270
                     /locus_tag="VGT44_04960"
     CDS             749..1270
                     /locus_tag="VGT44_04960"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV2580183.1"
                     /translation="MDEFLNYVLLLVLLDTGMRVSELCGLRVEDVLVSGSQGAFVRVF
                     GKGRKEREIGLHPEVSKLLWKYIQKYRHPNHPDEKALFLGRRGEPFQVSGINSLLKRV
                     KKASGIDGVRVLAHTFRHTFAKYYLKRGGEVFKLSREMGHSTVQVAEVYLFRHMSDNL
                     TEFFQYRASFKEH"
     gene            1430..2917
                     /locus_tag="VGT44_04965"
     CDS             1430..2917
                     /locus_tag="VGT44_04965"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2580184.1"
                     /translation="MTRLNEAAMRRLIKGGETTTVEFKVATPRPVEMAERLCGMANAQ
                     GGMIIIGIEDVERKVVGVPDERMAMTKDVILRAARQIIKPALMLAPPEPEIYEIDGKQ
                     LVVVTVPPNKGTVYQASGVFWVRRSTHTVPLSASELIEMANDRGLVRWELLPARKAEM
                     KDIDLEKVEAYLRQRSMRSRQARRFEDIEQVLIGMECAVVLDDGKIVPTNAGILFFGH
                     EPQMHIMQSEIDCVLFRGAMGTSRYTDKKVITGTIQELIDGAETFLRSYIPVEGRVEG
                     WKRIDIPEYPVEALREALVNAVVHRDYSRYGESIRVFYYADRIEIHSPGLLLPGVTVE
                     QMEKGEVQSRLRNSVLANLLRDVPGYMERIGSGIRFMLDETRRMGLAAPHFREIDEFI
                     VTFRSARISPTVRPHEPQVSKTLWDEDEQLSALPASTEDLSDKEQRLEKAIMYVHEHG
                     FITNSLYRVITGVTEKRAFRDLEVLVERGSLKCVGSKRGRRYELS"
     gene            complement(3195..3277)
                     /locus_tag="VGT44_04970"
     tRNA            complement(3195..3277)
                     /locus_tag="VGT44_04970"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3242..3244),aa:Leu,seq:taa)
     gene            3465..5417
                     /locus_tag="VGT44_04975"
     CDS             3465..5417
                     /locus_tag="VGT44_04975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV2580185.1"
                     /translation="MQSYAVLEEVATNLRSGLILLDTQEQVAFSNRSAERLLGVNLRE
                     MLDGPVFDVRAHLLTLAAQPGDALVELERLWLHTDEEQTTDLALADAAVRWLRVHSFP
                     VRDEPGHLLGRGVLLDDSTLEHEAAQASSETFAMAAHELKTPLAVIKGCATTLLGNAP
                     RWDPALIREMLQMIDTQADRLHDVIHTLLDVWRLDAGVQSLRLAEVRLTELLASIVER
                     WRKNAPRHTFNLRLPDQVVTVRCDALRIEQAIIHLLSNAVSYSPLGSVVTARLETNET
                     EIRLSISDQGIGIAPEHLERIFTRFYRVPHALLAPQGYERPGGNGLGLAVARATIEAH
                     GGSIWAESAGTGHGATFYCILPRCAEQGQRLAHPAPATYSMASSASSSTSPAASVASA
                     AASITTPHATESTGTLPLPRTGPLRAQQHASIAVVEHDPRMARYIRANLEEQRYQVYT
                     ASHGLQFLRQLDLQEPDLALLASDVGDMSGAELLEHLREFSHIPVLMLCDDNEDEDAR
                     VRYLDLGADDFVVKPLSMKELLARVRVLLRRRGARDDAASEPVFTSGDLVIDFAQHQV
                     FAQGRAVQLSRTEYKLLSVMAQNAGRVMTHELLLERVWGAEYSREVDFIWVYISRLRR
                     KIEADPRHPRYILTVPDVGYKLAKLS"
     gene            complement(5423..6469)
                     /gene="ruvB"
                     /locus_tag="VGT44_04980"
     CDS             complement(5423..6469)
                     /gene="ruvB"
                     /locus_tag="VGT44_04980"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV2580186.1"
                     /translation="MTERLVSPKVTEEEQVIEGSLRPRRLGEYIGQEKIKDNLSILLE
                     AARRRKESVDHVLLYGPPGLGKTTLCNIIAVEMGVNLRTTSGPAIEHAGDLASILTSL
                     QQGEVLFIDEIHRLARAVEERLYSAMEDFAIDVVIGKGPGARSIRLSLPPFTVVGATT
                     RVGSLTAPLRDRFGAIYRMEYYDPAALKQILTRSARILNVPAEEDGIEEISSRARGTP
                     RIVNRLLKRVRDYAQVRANGIITLDVARAALNALEIDQIGLDQTDRNLLLTIIQKFDG
                     GPVGLDTLAASTSEDSETIEDVYEPYLLQLGFIARTPRGRITTRLAYKHLGLDAPHGG
                     NGIGPGLWEAPDAG"
     gene            complement(6790..7476)
                     /locus_tag="VGT44_04985"
     CDS             complement(6790..7476)
                     /locus_tag="VGT44_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2580187.1"
                     /translation="MTEPQHPSSDGTNTGTTSHRFFAAVYERVSRSGSESRFMEPLRK
                     EIMGQACGVVLEIGAGNGLNFALYDPAQVERVEAIEPDSAMLRYAHERVKAARVPITI
                     TQTPVERLPFADETFDSAVCTLVFCSVTDPLRGLQEIRRVLKPGGALLMVEHVRAHGR
                     LAVATQNIITPFTRLVAGNCHWNRATEQAVYQAGFQPIERRDFEGGIMPVVLLRVTTP
                     SRDPLLYGAS"
     gene            complement(7548..9833)
                     /gene="recQ"
                     /locus_tag="VGT44_04990"
     CDS             complement(7548..9833)
                     /gene="recQ"
                     /locus_tag="VGT44_04990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEV2580188.1"
                     /translation="MDHIEQALKAQFGHDTFRAGQREIIEHILAGDDAFVLMPTGGGK
                     SLTYQLPALLLPGLTIVVSPLIALMHDQVDRLRANGIAATFINSAISGTERDERERLA
                     LNGQLQLLYVAPERLVTANFLAMLDEVDQRFGLSLVAIDEAHCVSEWGHDFRPEYRQL
                     SIMRTRYPRVPLVALTATATERVREDILTQLRLRDPHIHIASFNRPNLSYEVREKRQG
                     SYGQLAQLLRSLDGASAIVYCQSRKSVDELSTTLKADGINALPYHAGLTSEQRTEHQE
                     RFIRDDVPVLVATIAFGMGIGKPDIRAVIHYDLPRNLEGYYQESGRAGRDGLPAQCVT
                     FFSHGDRAKVEYIIGQKIDEQEQRIAHQQLQQVIHYCETSVCRRKVLLAYFGESLPDG
                     QCDNCDNCSQPMLVEDRTRDAVTFLFGVAKTQQRFGIRHVVDVLRGANTERIRANQHD
                     QLPIYGYGKALSVEEWLRIGRSLIHQGLLSETSDGYPILRLNTLSWEVLRKERAVQIA
                     TPVKPTKPRAGQAEKARKAVKEIASEEAVPDEPGTSALFHHLRNLRKAIADEQRVPPY
                     VIFPDTSLRDMARWRPQTEEQFLDIPGVGQKKFEAFYTPFTREIRAYCEEHNLRVGLR
                     TPDMLKASRLAKAQADEPDEVQVKPKAEKPDKPAPALTENVTRHLSRAMFQQGLSVDE
                     IAKRRNLTRGTILTHLSELLEAGEVIDIERLIAPERYEAIAEALHRIGYSTLRPIKDD
                     LGDDYSYDEIRLVRAAIRISS"
     gene            complement(9864..10526)
                     /locus_tag="VGT44_04995"
     CDS             complement(9864..10526)
                     /locus_tag="VGT44_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580189.1"
                     /translation="MPRDEQRRQKALMKKRSKQKAAKQHSHAQSFASLSAQSILRQAR
                     TYPVYECMISPTWQKDQPGLVEIVLARQQPDGDICFGVYLVDKLCLGLKNTFANVNFS
                     RSRYQSEIHDKIAGRSGLKECPLELAHQMIYASIDYARQFGFEPNKDFALSQFLLLPR
                     GELQEPYQITFGKDGKPLYISGPNDNPQRIIRQLEKTAGPGNYDYIAMLGDPGGDFDD
                     DI"
BASE COUNT         2217 a         2720 c         3323 g         2453 t
ORIGIN      
        1 taacggcggc aagcccctga gcgcgcagtt catcgctgag cgactctctc tcccgcttgc
       61 ccaggtgatc gccatacttg atgacctgca acaacacctg gtcttcctgg tgcagcagcc
      121 cgccggtgaa gtccgctggg cttttcccgt cactgtagaa ccaacgccac accgcctgcg
      181 cttcagcagc ggcgaacgca tctatgccgc ctgagcggag gatgcgctcg ctacgccctt
      241 cgtgcaaggg catttaggtc atgagaacct ctccgtcgaa atagcaactg cctgcgccca
      301 ttgcggccag cctattcata tcggcctgga tagcgcgttg cgctggagca tccaggagcc
      361 ggacgccgcc ccgctcgtct tcatgccaga tgtagactgg ggccgtttca ctgaaccaac
      421 gatccttgat gcctattgaa ggaattcggt tttcttctgg tcagaagaac acgcgagagc
      481 gttccgggag aagcagcagc acgtggatgg gttgtatttg acgatggatc aggctgtgtt
      541 tgtgacgagg atagggcagg ggaggttgtt tgggtttggt acttgatctt catctcaatc
      601 ataccacaac ttcatcatta ctctccctgg ggttaatgtg tcctcaatga tggctgtatc
      661 ataatattaa tgaggactga taagcttcaa ttgagtattc cgccataatc cgccatacat
      721 gcttccaatg gcgggataga tccgccaaat ggacgaattc cttaactatg tactgctcct
      781 ggtgctgctt gatacgggta tgcgtgtttc agaactgtgc ggtttgcgag tagaggatgt
      841 gcttgtatcc ggttcgcaag gcgcatttgt cagggtcttt ggcaagggac ggaaagaacg
      901 tgagataggg ctacatccag aggtaagtaa actgctctgg aaatatattc agaaataccg
      961 tcacccgaat caccctgatg aaaaggcgct ctttcttggc aggcgcggtg agcctttcca
     1021 ggtgagcggc atcaactcgc tgttaaagcg cgtgaagaag gcgagcggta ttgatggtgt
     1081 gcgcgtcttg gcgcatacct ttcgccacac cttcgcaaag tattatctca agcgcggcgg
     1141 tgaggtcttc aagctgtcgc gtgagatggg gcatagtacc gttcaggtgg cggaagtgta
     1201 cctctttcgc cacatgtccg ataatttaac cgagtttttc cagtatcggg catcctttaa
     1261 ggagcattaa tggacttgaa acttaaaaag tgcatggaag taggttatat cggacaggaa
     1321 tatcggacga attatcggac atgtggcgat aaaggattgt ttgaagttac atatgcctgc
     1381 tacaataagg tcgttcaagg agaataaatg ctattctgcg gaggtttgta tgacacggtt
     1441 aaatgaagca gctatgagac gtttgattaa gggtggcgag acgacgacag tcgagttcaa
     1501 agtagcgact cccagacctg tagagatggc tgaaagattg tgtggtatgg ctaatgctca
     1561 gggcgggatg attattattg ggattgagga tgtagagcgt aaagttgtgg gtgtgcctga
     1621 tgagcgcatg gctatgacaa aagatgtgat tttaagagct gctcgccaga ttattaagcc
     1681 tgcgctgatg ctcgcccccc ctgagcctga gatttatgag atagacggga aacagttagt
     1741 cgttgttacg gtgcctccaa ataagggcac tgtctatcaa gctagcgggg ttttttgggt
     1801 aagacgcagt acgcatacag taccgttaag cgcctctgaa ttaatcgaaa tggcaaatga
     1861 ccgtgggctt gtccgttggg aattgctgcc tgcgcgtaag gcagagatga aggatattga
     1921 tttagagaag gtagaagcgt atttacgaca gcgttcgatg aggagtcggc aagcccgtcg
     1981 tttcgaggat atagagcaag tattgatcgg tatggagtgt gctgttgtcc ttgacgatgg
     2041 aaaaatagtt cctacgaacg caggcatatt gttttttggg catgagccac aaatgcatat
     2101 aatgcaaagt gagatagatt gtgttttgtt tcgtggggca atggggacaa gccgatatac
     2161 tgataagaag gttattacag gaacgataca ggaattaata gatggcgcag agacctttct
     2221 tcggagctat ataccagttg aaggaagagt tgaagggtgg aagaggattg atatacctga
     2281 gtatcctgtt gaggcactac gagaggcgct cgtaaacgct gtcgtgcatc gggattatag
     2341 tcggtatgga gaaagtatac gagtgtttta ttacgccgat cgcatcgaga tacatagtcc
     2401 agggttgctg cttccggggg ttacagttga acagatggaa aagggtgagg tgcaatccag
     2461 gctgcgcaac tctgttttgg caaatttgct tagggatgtt ccgggctaca tggaacgaat
     2521 tggaagtggc attcgattta tgttggatga gacaaggcgt atgggtcttg ctgcccctca
     2581 ctttcgggaa attgatgagt ttatcgttac ttttcgtagt gctcgtattt caccaaccgt
     2641 gcggccacat gagccacagg taagtaaaac tctgtgggat gaggatgagc aattgtcggc
     2701 tttacctgct tcaactgagg acctttctga taaagagcag aggctggaaa aagcgataat
     2761 gtatgttcat gagcatggtt tcatcaccaa tagtttatat agggtaataa cgggcgtgac
     2821 agagaagaga gcttttcgtg atttagaggt attagtagag cgtggaagtt taaagtgcgt
     2881 aggttccaaa cgaggacgac ggtacgaatt gtcataaacc tcttaggctc gccgtgagca
     2941 taggacgtat tcgccgattg atgcgattgg gtcggtggga aagaagagga gaaggaagag
     3001 aagacagcag caatagcggt gtctccgcga aaaaaacgcg ataaatatgg ctctccgaaa
     3061 cggggatttg tgagtccccg ttgtggaggg ctttttgtcc acaacggaga ctttataaaa
     3121 atgcagaaaa aacgagtctc cgttgttgag actcttgttt gtgggttggc tctagaggcg
     3181 cttccagtgg gggatagggc gggagggaat cgaaccctca tgacctctcg gcccggagat
     3241 tttaagtctc ctgcgtctac cgtttcgcca ccgccccgta gtggtgtggg atgatattaa
     3301 catgctgcgc ggtattcgtc aatcatattg ccgggttgca atctatgggc aatttgggta
     3361 aaccttgacc tgtttgctag aatatcatta gcatagacat atctggcaca ggggaaaggt
     3421 attatctatt tatggatggc gccgatggag tagggtcagt atctttgcaa tcgtatgcgg
     3481 tgcttgaaga ggtggcaact aatctgcgca gtggtcttat tttactggac acgcaagagc
     3541 aagtggcgtt ctcgaatcgg agcgcggaac gcttgctggg cgtcaacctg cgtgagatgt
     3601 tagatgggcc ggtctttgat gtgcgcgcgc acctgctgac gcttgccgct caaccgggag
     3661 acgccctggt ggagcttgag cgcctgtggc tgcataccga tgaagagcag acgaccgacc
     3721 tggccctggc cgacgcggct gtgcgctggc tgcgcgtgca tagcttccct gtgcgcgacg
     3781 aacccggaca cctgctgggg cgcggcgtgc tgctcgacga tagcacgttg gaacacgagg
     3841 cggcgcaggc cagcagcgaa acctttgcga tggcggccca cgaactcaag actccgcttg
     3901 ccgttatcaa gggctgcgct acgacgctgc tcggcaacgc tccgcgctgg gacccggctt
     3961 tgatacgcga aatgctccag atgatcgata cacaggccga ccgcctgcac gatgtgattc
     4021 atacattgct ggatgtctgg cggctcgacg cgggcgtgca gtctttacgt ctcgcagagg
     4081 tgcgtctcac ggaactgctg gcttcgattg tggaacgatg gcgtaagaac gcgccccgcc
     4141 ataccttcaa tttgcgcctg ccggaccagg tggtgacggt tcgctgtgac gcgctgcgca
     4201 ttgaacaggc gattatacac ctgctgagta acgccgtgag ctattcgccg ctcggcagcg
     4261 tcgtgaccgc acggctagaa acaaatgaga ctgaaatccg cctgagcatt agcgatcagg
     4321 gtatcggcat cgccccggag catctcgaac gcatcttcac acgcttctac cgtgtgccgc
     4381 acgcgctgct tgctccacag ggctatgagc gacctggcgg caacggattg gggctggccg
     4441 tggcgcgtgc gacgattgag gcgcatggcg gcagcatctg ggccgaatcg gcgggaacgg
     4501 ggcatggcgc aacgttctac tgcatcctgc cacgctgcgc ggagcaaggc caacggctcg
     4561 ctcatcctgc cccggcaacc tactcaatgg cttcctctgc atcatcatcg acatcgcccg
     4621 ccgcgagcgt cgcctcggcc gcggcatcga taactactcc tcatgctact gaatcgaccg
     4681 ggacgctgcc gttgccgcgc acaggtccgc tgcgagcaca gcaacatgcc tctatcgcgg
     4741 tagttgaaca tgatcctcgc atggcgcgct atatccgcgc caacctggaa gagcagcgct
     4801 atcaagtgta taccgccagt catggcctgc aattcttgcg ccagcttgat ctgcaagaac
     4861 ccgacctggc gctgctggca agcgatgttg gggatatgtc gggcgctgaa ttgctggaac
     4921 acctgcgcga attttcgcat atccccgtgc tgatgctctg cgacgataat gaagacgaag
     4981 atgcgcgcgt acgttacctg gacctgggcg cggacgattt tgtggtcaaa ccattgagca
     5041 tgaaggaatt gctggctcgc gtgcgcgttc tgctgcggcg gcgaggtgcg cgcgacgatg
     5101 ccgccagcga accggtcttc acgagtggcg acctggtaat tgactttgcc cagcaccagg
     5161 tcttcgcgca gggtcgcgcc gtgcagctca gccgcactga gtacaagctg ctaagcgtga
     5221 tggcgcagaa cgccgggcgt gtcatgactc atgaactgct gttggagcgc gtctgggggg
     5281 cagaatattc tcgcgaagtc gatttcatct gggtctacat cagccgcctg cgccgcaaga
     5341 tcgaagctga cccgcgccac ccgcgttata ttttgaccgt ccccgatgtt ggctataagc
     5401 tggcgaagct atcatagata ggctatcctg catccggggc ttcccacaat cccggcccga
     5461 tcccattgcc gccgtgcggc gcatccagcc ccaggtgttt ataggccagc cgggtcgtga
     5521 tcctgccgcg tggggttcgg gcgatgaagc cgagttgcag caggtagggt tcgtagacgt
     5581 cttcgatagt ttcggagtct tcgctggtgc tggcggcgag ggtgtcgagg ccgacggggc
     5641 cgccgtcgaa tttctggatg atggtgagca gcagattgcg gtcggtctgg tcgaggccga
     5701 tctggtcgat ttccagggcg ttcagggcgg cgcgggcgac atcgagtgtg atgatgccgt
     5761 tggcgcgcac ctgggcgtag tcgcgcacgc gcttgagcag gcggttgacg atgcggggtg
     5821 tgccgcgcgc gcggctggag atttcttcga tgccgtcctc ttccgccggg acgttgagga
     5881 tgcgcgctga acgggtcagg atttgcttga gtgcggcggg gtcatagtat tccatgcggt
     5941 agattgcgcc gaaacggtcg cgcaggggcg cggtaagcga gccgacgcgc gtggttgcgc
     6001 cgacgacggt gaagggcggc agcgagaggc ggatcgagcg cgcaccggga cccttgccga
     6061 tgaccacgtc gatagcgaaa tcctccatcg cggaatacag gcgttcctcg actgcgcgcg
     6121 ccaggcggtg aatctcgtcg atgaacagta cttcgccctg ttgcagcgag gtcaggatgg
     6181 aggccaggtc gcccgcgtgt tcgatggccg ggccggaggt ggtgcgcagg ttgacgccca
     6241 tctcgacggc gatgatgttg cagagcgtgg ttttgcccag acccggcggc ccgtagagca
     6301 gcacgtgatc gacggactct ttgcggcggc gcgccgcttc cagcaggatg ctgaggttgt
     6361 ccttaatttt ctcctgcccg atgtattctc ccaggcgacg gggtcgcaaa ctgccctcga
     6421 tcacctgctc ttcctcggtc actttgggtg aaacgagccg ttcggtcatt gtgcgctcct
     6481 ccgcattaat gaaacgagtg ttctattgtt tcctgtagac attatatcag agttgagcgc
     6541 gcgcgtcgag ggaaaacgct ctcacttttt cattcgtgta atgcatacgg ggcgggtatg
     6601 tttgtaaaaa ggtaaggcat agtatcctta tggattatag aaaaggaggc gtatccatgc
     6661 caggaaagaa gaaagcgctc ctgaagggcg tttcacctaa agaagaaaga atgtacgagc
     6721 atatcaaaga gtcctcggaa aaatccggtc gctatggcag ccgggcgaag gaggttgcgg
     6781 ctcgtaccgt tatgaagcac cataaagaag agggtcacga gaaggggtag taaccctcaa
     6841 aagtaccacc ggcataatgc cgccttcaaa atcgcgccgt tcgatgggct ggaaaccggc
     6901 ctggtagacg gcttgttcgg tggcgcggtt ccagtggcag ttacccgcga cgagccgcgt
     6961 gaagggcgtt atgatgttct gagttgccac ggcgagacgg ccatgcgcgc gtacatgctc
     7021 caccattagc agcgcgccgc ccggcttgag gacccgacga atctcttgca gaccgcgtaa
     7081 ggggtcggtc actgagcaga agaccagcgt gcagacggcg ctgtcgaagg tttcgtcggc
     7141 gaaaggcagg cgctcgactg gcgtttgtgt gatggtgatg gggacgcggg cggctttgac
     7201 ccgctcgtga gcgtagcgca gcatggcgct gtccggttcg atggcctcca cccgctcgac
     7261 ttgcgccggg tcatataagg cgaagttcag gccgttgcct gccccgatct ctaacactac
     7321 tccacacgcc tgccccatga tctctttgcg cagtggctcc atgaagcgac tttcggagcc
     7381 gctgcgcgag acgcgctcgt aaacggcggc gaagaagcga tgtgaagttg tgccggtatt
     7441 ggttccgtcg ctagacgggt gttgcggttc ggtcattggc gctacgcatc cctatctgtg
     7501 ttgtgtatat gtctatagta tcacaggcaa aggagaccga tgcacaccta cgatgaaatg
     7561 cgtatggcgg cgcgcaccag gcggatctcg tcgtatgaat agtcgtcgcc gaggtcgtct
     7621 ttgatggggc gcaaggtgct gtagccgatg cggtgtaatg cttcagcgat ggcctcgtag
     7681 cgttccgggg cgatgaggcg ctcgatatcg atgacttcgc cggcttcgag taactcggag
     7741 aggtgggtga gaattgtgcc acgtgtcagg ttgcggcgtt tggcgatttc atcgacgctc
     7801 agcccttgct ggaacatggc gcggctcaag tggcgcgtga cgttttcggt cagggcgggg
     7861 gccggtttat caggcttctc tgcttttggc ttcacctgaa cctcgtccgg ttcgtctgct
     7921 tgggctttcg ccagcctgga tgctttgagc atgtccgggg tgcgcaggcc gacgcgcaag
     7981 ttatgctctt cgcaataggc gcgaatctcg cgggtgaagg gcgtgtaaaa tgcctcgaat
     8041 ttcttctggc ccacgccggg gatgtccagg aattgctcct cggtctgggg tcgccagcgc
     8101 gccatgtcac gcaggctggt atcggggaaa atgacgtagg gcggtacgcg ctgctcgtcg
     8161 gcgatggcct tgcgcagatt gcgcaggtgg tggaagaggg cgctcgtccc aggttcatct
     8221 ggcacggcct cttcgctggc gatctctttc acagctttgc gcgccttctc cgcttgcccg
     8281 gcgcgcggct ttgtgggttt gacgggcgtg gcgatctgga cggcacgctc tttgcgcagg
     8341 acttcccagg agagcgtgtt gaggcgcagg atggggtagc cgtcgctcgt ctcgctcagc
     8401 agaccctggt ggatgagcga gcgcccgatg cgcagccatt cttccacgct gagcgccttg
     8461 ccatagccgt agatgggcag ctggtcgtgc tggttggcgc gaatgcgctc tgtattggcc
     8521 ccgcgcagca catcgacgac atgacgaatg ccgaaacgct gctgtgtctt ggctacgccg
     8581 aagaggaagg taacggcgtc acgtgtgcga tcctcgacca gcatcggctg ggagcagtta
     8641 tcgcaattgt cgcactgacc gtcgggcaga ctttcgccga aataggccag cagcaccttg
     8701 cggcggcaca cgctcgtttc gcagtaatga atgacctgct gcaactgctg gtgggcgatg
     8761 cgctgctcct gctcgtcgat tttctggccg atgatgtatt cgactttggc gcggtcgccg
     8821 tgactgaaga aggtcacaca ctgcgcgggc aggccatcgc gcccggcgcg gcccgattcc
     8881 tggtaatagc cttccaggtt gcgcggcaga tcgtagtgga ttacggcgcg gatgtctggc
     8941 ttgccgatgc ccatgccgaa ggcgatggtg gccaccagca ccggcacatc gtcgcggatg
     9001 aagcgttcct ggtgttcggt gcgctgctcg ctcgtcagtc cggcgtgata ggggagagcg
     9061 ttgataccat cggctttgag cgtggtactg agttcgtcaa cgcttttgcg gctctggcaa
     9121 tagacgatgg cggacgcgcc atccaacgag cgcagtaatt gcgccagctg tccatacgac
     9181 ccctggcgtt tctcacgcac ctcataagaa aggttggggc ggttgaagct ggcgatgtgg
     9241 atatgcgggt cgcgcaggcg cagctgcgtc aggatatcct cgcgtacacg ctcggtggcc
     9301 gtcgctgtga gcgccacgag cggaacgcgc gggtaacggg tacgcatgat gctgagctgg
     9361 cgatattccg ggcggaaatc atgcccccat tcgctgacgc agtgcgcctc gtcgatggcg
     9421 accagcgata ggccgaagcg ctgatctacc tcgtcgagca ttgccaggaa gttggccgta
     9481 accaaccgct caggcgcgac gtagagcagt tggagctggc cgttcaaggc gaggcgttcg
     9541 cgctcatcgc gttctgttcc gcttatggcg ctgttgatga aggtcgcggc gataccgttg
     9601 gcacgcaggc gatcaacctg gtcatgcatc agcgcaatca gcggcgaaac gacgatggtc
     9661 agccctggca gcagcagcgc gggcaattga taggtcaggg atttgccacc gcccgttggc
     9721 atcagcacga aagcgtcatc gcccgccaga atatgctcga tgatctcgcg ctggcccgcg
     9781 cgaaaggtgt catgcccgaa ttgcgccttc agcgcctgct cgatgtgatc cacgccctgt
     9841 atccctgcac tctctgtttg gaattaaatg tcgtcgtcga aatcgccacc aggatcaccc
     9901 agcatggcta tgtaatcata gtttcctggc cctgccgtct tctcaagttg tctgatgatg
     9961 cgctggggat tgtcgtttgg cccggagata taaaggggtt tcccatcttt cccgaacgtg
    10021 atttgatacg gctcttgaag ctcgccacga ggcaggagca gaaattgcga caaggcaaaa
    10081 tccttgtttg gctcaaagcc gaactgcctg gcgtagtcaa tcgaggcgta gatcatctgg
    10141 tgcgccagtt cgagcgggca ttccttcaat cccgaccgcc cggcaatctt gtcatgaatc
    10201 tcgctctgat aacgactgcg cgaaaagttc acgttggcga aggtattttt gagacccagg
    10261 cacagcttat cgacaagata aacaccgaag cagatatcgc cgtcgggctg ctggcgcgcc
    10321 agcacgattt ctaccagccc cggttgatct ttctgccagg tggggctaat catgcattca
    10381 tagacgggat aggtgcgcgc ctggcgcaga atgctctgcg cggagaggga ggcgaatgat
    10441 tgggcatggg agtgctgttt ggcggccttt tgtttgctgc gcttcttcat gagcgccttc
    10501 tggcgacgtt gttcatctcg tggcatatcg atttcctctt ggtaactgat tagcagcgaa
    10561 cttatgatat atcatctgat gaaactattc aatagtgcga attgactcac caaaaatctt
    10621 actttggccg aaacgttgcc tcatgagaga atcttaccag ccgctgttgt ggaagagcca
    10681 ggcggctgga aggaagagag gccatgatga gtg
//
LOCUS       DASZAK010000285         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_397873, whole genome shotgun sequence.
ACCESSION   DASZAK010000285 DASZAK010000000
VERSION     DASZAK010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397873"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /locus_tag="VGT44_05000"
     CDS             <1..136
                     /locus_tag="VGT44_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580190.1"
                     /translation="ETPIGTCMVSPEGACAAYFNYGRFSMASERSRLNVLPTANAARQ
                     "
     gene            161..1273
                     /gene="hypE"
                     /locus_tag="VGT44_05005"
     CDS             161..1273
                     /gene="hypE"
                     /locus_tag="VGT44_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEV2580191.1"
                     /translation="METGGSRDTNRIDNVLKKIERSRQARRHKFYLRDEKITLSHGSG
                     GKSTHNLIEGVFAPAFSNPMLDAMDDAATFSLNGSGQRMAFTTDTYVVSPLFFPGGDI
                     GKLAVHGTINDLAMAGATPLYLSTGFILEEGFPIADLRRIVESMANAAREAGVSIVTG
                     DTKVVQRGKADGLFINTAGVGIMRGSWQPGQAYIQPGDVVLLSGSVGDHGIAIMLARE
                     ALEIETDVQSDTAPLHSLVAAIYDVAGESIHCLKDPTRGGVATSLNEMALGAEVAIGL
                     DEHAVPVHAGVRGACEILGLDPLTIANEGKLLAVVAPDKAEAALAAMRRHPLGREAAI
                     IGAVQDDPAGMVFLRTDIGGMRVLDMLVGDPLPRIC"
     gene            complement(1296..2009)
                     /locus_tag="VGT44_05010"
     CDS             complement(1296..2009)
                     /locus_tag="VGT44_05010"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2580192.1"
                     /translation="MTQRAFKPVLLDLLRQAQISQNAFFQKLSPAELATIGAPEFWSA
                     KDHVAHMTFWRQRLAFKVQAIVRQEAQPQALDFEQLNPVIFEEQRYRSWPDILAESDR
                     VYDELITLTDQLTEEDLTAFNRFDWSPDGAPLYTAYMGNCYEHTQQHLAQFAFDRHDP
                     ERAIDTFEAWTRRVLEAEVPETLKGYLYYNLACFYATHALLEKAAQPLEQAFALYPPF
                     REFALTDPDLVAFRPDQPE"
BASE COUNT          465 a          605 c          648 g          454 t
ORIGIN      
        1 ggagacgccc atcggcacct gcatggtatc gcctgagggc gcctgcgccg cctacttcaa
       61 ctatggccgc ttctcgatgg cgtctgaacg ctcacgtctg aacgtactcc caacggcaaa
      121 tgcggccagg caataacaat aacttccgca ggaggtttct atggagacag gtggatcgcg
      181 tgacacaaat cgcatcgaca acgtgctgaa aaagattgaa cgttcgcgac aggcgcgtcg
      241 ccataaattc tacttgcgcg acgagaagat caccctcagc cacggcagcg gcggcaaatc
      301 cacgcacaac ctgatcgaag gcgtcttcgc ccccgccttc tcgaatccaa tgctcgatgc
      361 aatggatgat gccgccacat tttcgctgaa tggcagcggc cagcgtatgg ccttcacgac
      421 ggatacctac gtcgtcagcc cgctcttctt ccctggtggc gacatcggca aactggccgt
      481 gcatggcacg atcaatgacc tggcgatggc aggcgcaacc ccgctctatc tctctacagg
      541 ctttatcctt gaggaaggct ttcctatagc cgatctgcgc cgtatcgtcg agtcgatggc
      601 gaacgcggcc agggaagccg gtgtgtccat cgtgaccggc gatacaaagg tcgtccagcg
      661 tggcaaggcc gatgggctgt tcatcaacac ggctggcgtt ggcatcatgc gcggatcgtg
      721 gcagccgggg caggcgtata ttcagccggg agacgtagta ttgttgagcg gttcggtcgg
      781 agatcacggc atcgctatca tgcttgcgcg cgaagccctc gaaatcgaaa ccgatgtgca
      841 gagcgatacc gcgccgctgc attcactggt cgccgcaatc tacgacgtgg caggcgagag
      901 catccattgc ctgaaagatc ccacgcgcgg cggcgtcgcc acctcgttga acgagatggc
      961 gctcggtgcg gaggtggcca tcggactgga tgaacacgct gttcccgttc atgccggggt
     1021 gcgtggcgcc tgcgaaatcc tgggactcga tccgctgacg attgccaacg agggcaagct
     1081 cctggcagtg gtcgcgcccg acaaagccga agcagcgctg gccgccatgc gtcgccaccc
     1141 actggggcgc gaagccgcaa tcatcggcgc ggtacaggac gatcccgcgg gtatggtctt
     1201 cctgcgcacc gacattggcg gaatgcgcgt cctggatatg ctcgtcggcg acccgctgcc
     1261 gcgtatctgc taaacgtgat acctcagtag gagagctact ccggctgatc ggggcgaaac
     1321 gcgacgaggt cgggatcggt cagcgcaaat tccctgaagg gaggatacag cgcgaatgct
     1381 tgctctaaag gctgagccgc tttttccagc aaagcatgcg tggcgtaaaa gcaggccagg
     1441 ttataataca ggtatccttt caaggtttct ggcacctcag cctctagaac tctgcgggtc
     1501 caggcttcaa acgtgtctat cgcacgttcg ggatcgtgac ggtcgaaagc gaactgcgca
     1561 aggtgctgtt gggtatgctc gtaacaattg cccatgtagg ctgtgtagag cggcgctcca
     1621 tctggagacc agtcgaagcg attgaaggcc gtcaaatctt cttctgtcag ttgatcggtg
     1681 agcgtgatga gttcgtcgta tacgcgatca gattcagcga ggatatctgg ccaggagcga
     1741 tagcgctgtt cttcaaagat gaccgggttg agttgctcaa agtctaaagc ttgcggctgt
     1801 gcctcctgac gaacgatggc ctgcactttg aacgcgaggc gctgacgcca gaacgtcatg
     1861 tgcgccacgt ggtctttggc gctccagaat tctggagcgc cgatagtagc cagttcggca
     1921 ggggacaatt tctgaaagaa ggcattctga ctgatctgag cttgacgcaa aagatcaagt
     1981 aaaacgggct taaatgcacg ttgggtcata gattttcttc ctcatttcat cattaagaac
     2041 acattatccc cattgtttgc gcaatctcgg caggatatcc ctggcataga gctggaggaa
     2101 gcgcgcctgg tcgtcgcctg gcgcgtggaa gatcaggtgt gtgaaaccca gtttgatgta
     2161 gtggcccact tg
//
LOCUS       DASZAK010000286         5621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_220327, whole genome shotgun sequence.
ACCESSION   DASZAK010000286 DASZAK010000000
VERSION     DASZAK010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220327"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VGT44_05015"
     CDS             <1..612
                     /locus_tag="VGT44_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV2580193.1"
                     /translation="CGARRQPTLADKSAVRTVNRRLRKMTDTRLSNQFRKAVGIDYMK
                     DMAPDMYDRLQILQTYRHVAIVGISADTSRPSHFVAIYLKAEGYHIVLVNPRYAGQTI
                     LGQPVYASLAEAKTAGELIEIVDVFRRPKDVPPVAEEAIQVGAKVLWLQLGIRNEEAA
                     QRAQQAGLIVVQDRCIKIEHARFFGGLHTVGLNTGVIMSKKLE"
     gene            complement(655..1767)
                     /locus_tag="VGT44_05020"
     CDS             complement(655..1767)
                     /locus_tag="VGT44_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEV2580194.1"
                     /translation="MMGEQEMQFADPDWKPLAQRQTQSGDVAAFVPQPVNSRAYDAGN
                     TGQPATSYEQGYRGPLRQEQTPYVPLGAQQMATPPAANTRRRSRWWVWVIILIVIFSI
                     INGANHSSSSYGPGSYPGPYYGRADAGQQTTSFDLANASLVHITDWADSIQLQQGDPS
                     SHQVVVQSDTANTDQPQEAMDGSTLTITVSPAQGGNTIIVTLPPGVAVSLETTSGDIE
                     VDGYSGPVSARTDTGAITLNSDTLSGSTVISSNSGDINLVQGSATGTTLLSTNSGSVT
                     LDQEALGGQFSTNVGATGTISVAGTLDPKGTYQFTTLEGNIDLALPADTSMQVHESTG
                     AGGSYSSDFPQSTGKTPQAALNLKTTIGAISIKAGG"
     gene            complement(1834..2931)
                     /locus_tag="VGT44_05025"
     CDS             complement(1834..2931)
                     /locus_tag="VGT44_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HEV2580195.1"
                     /translation="MNVNMTRGKRERMNACADDRGVFAALAVDHRGNLLQALAEARGP
                     GGQANAADMLAFKTAVTQVLTPYASAILLDPEYGLGAIPSRAPRTGVMLAYEQSGYYV
                     SDRLSGGRTQSVAGRNKITHDIETEGRLPDLLPEQSVRRLVEAGAQAIKILLYYNPYD
                     APRINSIKQAYVERIGAECAALEAPFFLEALVYDGALGDEKGPLFARRKPDYALRAIE
                     EFRRPRYGVDVLKVEMPVNPAFVSGTRAYNGAEAAYSRNEAAKILRETASAASLPLIY
                     LSAGVSNDVFCEMLELAAEAGARFSGVLCGRATWQEGIAIYGQQGDAALRSWLADRGA
                     QNIQAVNDILAGAATPWWDVYGGLEKSEITI"
     gene            complement(3052..4071)
                     /gene="iolG"
                     /locus_tag="VGT44_05030"
     CDS             complement(3052..4071)
                     /gene="iolG"
                     /locus_tag="VGT44_05030"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEV2580196.1"
                     /translation="MPAQRLKVGLIGAGRIGHLHAEHLASRIPSVDLLIVADVFVESA
                     RECAQRYDIPVYTQHYQEVLEHPDIQAVIICSSTDTHARIIKEAAGYGKHIFCEKPIA
                     LDLPSIDAALRAVERAGVKLHIGFNRRFDANYRRVRKAIQEGEIGQPLVIHLISRDPA
                     PPALEYLRVSGGIFLDMTIHDFDMARFLIGSEVEEVSAVASTLDQPGIASVGDVANTV
                     VTLRYANGVIGTISNSRAAVYGYDQRVEVLGSAGFISIGNNYPNTAIIADAHSVRRDL
                     PLYFFIERYTESFVSEMTAFVEAVRSGTPVAVTGLDGRIPVVMALAAQRSLGERRQVR
                     LDEIE"
     gene            complement(4113..5066)
                     /locus_tag="VGT44_05035"
     CDS             complement(4113..5066)
                     /locus_tag="VGT44_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2580197.1"
                     /translation="MIEIVSTIRVANAPCSWGALEFEGLQGEEVPYSRMLDELRDTGY
                     TGSELGDWGYMPTEAAALRAELERRRLALVGAFVPVALRREDAHAAGEAQALKVAHLL
                     VEAATGAAQGDQLPFLILADDNGADPLRTRHAGRVMPQMGLSQAEWRTFAGGAERIAR
                     SVREQTGLKTAFHHHCAGYVETPEEIARLLDMTDPELLGLVFDTGHYLFGSGRVDGAV
                     VLEGFERFGERVWHVHFKDLQPQVAAQSRAEEWDYFTSLGHGLFCELGQGSVPFSAFA
                     GQLRARSYRGWVVVEQDILPGMGSPAASSQRNRDFLKTIGL"
     gene            complement(5076..>5621)
                     /locus_tag="VGT44_05040"
     CDS             complement(5076..>5621)
                     /locus_tag="VGT44_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2580198.1"
                     /translation="NVGYGLDAGVEMGPVITSESKQRIESLIAKGTGEGAKVLVDGRG
                     ASIAGYERGYFVRPTILEDVDPRGELARTEIFGPVLSLMHTQTIDEAIELVNVTAYGN
                     MACLFTSSGAAARKFRYEAQAGNIGINIGVAAPMAFFPFSGWKESFFGDMHAQGRDAV
                     EFYTQKKVVVERWPKEWSRKF"
BASE COUNT         1146 a         1713 c         1591 g         1171 t
ORIGIN      
        1 tgtggagcgc gtcggcaacc aaccctggcc gataaatcgg cggtgcgcac ggtgaaccgg
       61 cgcctacgaa agatgaccga tacaaggcta tcaaatcagt ttagaaaggc ggtaggcatt
      121 gactacatga aagatatggc tcctgatatg tacgaccgtt tgcaaattct gcaaacctac
      181 cggcacgtcg ccatcgttgg catttcggct gatacctcac gtcccagcca ttttgtagca
      241 atttacttga aggcagaggg ctatcacatc gtcctcgtca acccgcgtta cgccgggcag
      301 actattctcg gtcagccagt ctacgcttcg cttgcggaag caaaaacggc tggcgagctg
      361 attgagatcg tcgatgtgtt ccgcagaccc aaagatgtgc cacctgtcgc ggaggaggcc
      421 atccaggtcg gcgcgaaggt cctctggctg caactgggca ttcgcaacga ggaagcggca
      481 cagcgcgcgc agcaggctgg tctgatcgta gtgcaggatc gctgcatcaa gatcgaacac
      541 gcccgtttct tcggcggcct gcacacggtg ggactgaaca caggcgtgat tatgtcgaag
      601 aaactggagt gaagcaagca cctttctaac acgcttcatc actacgctat agtttcagcc
      661 acccgccttg atacttatgg caccgatcgt agttttcaga ttgagcgccg cttgcggcgt
      721 tttccctgtg ctttgaggaa aatcgctgga atatgacccg cctgcccccg tcgattcatg
      781 cacctgcatg gaggtatcag cgggcaacgc caggtcgata ttgccctcga gagtggtaaa
      841 ctggtacgtt cccttcgggt ccaacgtgcc agcaacacta atagtgccgg tcgcgcccac
      901 gttggtcgag aattgaccgc caagggcttc ctggtccagc gtgaccgaac cgctattcgt
      961 gctaagcaat gtagtgccag ttgccgagcc ctgtaccagg ttgatatcac cagaattcga
     1021 gctgatgaca gttgacccac tcaacgtgtc gctattgagc gtaatcgcac cagtgtcggt
     1081 cctggctgat accgggccgg agtaaccatc cacctcgata tcacctgagg ttgtctccag
     1141 agagacggca acgccgggag gaagcgtcac gatgatcgta ttcccgccct gcgccggact
     1201 caccgtaatc gtgagtgtgc ttccatccat tgcttcctgg ggctggtcag tgttcgcggt
     1261 atcgctttgc actaccactt gatgtgaact cggatctcct tgctggagct ggatagaatc
     1321 ggcccagtca gtaatatgaa ccagcgacgc attcgccagg tcaaagctgg tggtttgttg
     1381 gccggcatcg gctctgccat aatacggccc tgggtatgag ccaggtccat atgatgaaga
     1441 ggagtgattc gcgccattga tgatggagaa aataacgatc agaataatta cccacaccca
     1501 ccacctggaa cggcggcgcg tgttcgctgc cggaggcgtc gccatctgct gcgcgccgag
     1561 agggacatag ggcgtttgct cctggcgaag cgggcctcga taaccctgct cataggacgt
     1621 ggcaggctga cccgtattac ccgcgtcata cgcacggctg tttaccggtt gggggacaaa
     1681 cgcagctaca tcgccagact gagtttgcct ctgcgcaaga ggtttccaat cgggatcggc
     1741 gaattgcatc tcctgctcgc ccatcatcgt tctccctttt tacgacatgc ctatttcctt
     1801 ctcctattat atacgataat ggcgataaga gggtcaaatc gtaatctcgc tcttctccag
     1861 accgccatag acatcccacc agggcgtggc ggcaccggcc aggatgtcgt tgaccgcctg
     1921 gatgttttgc gcgccgcgat cggcgagcca gcttcgcagc gccgcgtccc cctgctggcc
     1981 atagatggcg atgccttcct gccacgtggc gcgcccgcac agtacgccgg aaaacctggc
     2041 tccggcttcg gcggcaagct ccagcatttc acaaaatacg tcgttgctca cgccggcgct
     2101 gaggtaaatc aaggggagcg atgcagcgct ggccgtttcg cgcaggattt ttgctgcttc
     2161 attgcgcgaa tacgcggctt ccgcgccatt gtaggcgcgc gtaccgctga caaaggcggg
     2221 attgaccggc atctcaactt tgagtacatc gacgccgtag cgcggcctgc gaaactcttc
     2281 gatggcgcgc agcgcgtagt ccggttttct cctggcaaag agcggccctt tctcgtctcc
     2341 gagcgcgccg tcgtaaacga gcgcctcaag aaaaaaaggc gcttccagcg ctgcgcattc
     2401 agcgccgatg cgttccacat atgcttgctt gatactgttg atgcgtgggg catcataggg
     2461 gttgtagtac agcaggattt tgatggcctg cgccccggct tcaaccagac ggcgtacact
     2521 ctgctccggc agtagatcgg gcaagcgtcc ctcggtttct atatcgtggg tgatcttatt
     2581 ccgaccggca acggactgcg tcctacctcc gctcagccgg tcggagacgt agtagcccga
     2641 ctgttcataa gccagcatca cgcccgttcg gggcgcgcgg ctgggaatgg cgcctaggcc
     2701 atattcgggg tccaggagga tggcgctggc gtagggtgtc aatacctgcg tcacggcggt
     2761 tttgaatgcc agcatatcgg cggcatttgc ctggccgccg gggccgcgcg cttcggcaag
     2821 agcctgcaag agattgccgc gatgatccac cgccagcgcc gcaaacaccc cgcggtcatc
     2881 ggcgcaggcg ttcattcttt cacgtttccc acgagtcatg ttgacattca tgcattatat
     2941 cccctcatga attttcacaa actagatcgg caatgtcatc ccttcacttc gttcagggca
     3001 agctctgagc gcagcgaagg atctcggtcc ccggcgcatc agattttcta ttcattctat
     3061 ctcatcaagg cgtacctgtc tgcgttcgcc gagcgaacgc tgagccgcca gcgccatcac
     3121 gaccggaata cgcccatcca ggccggttac tgctaccggc gtaccggaac gcaccgcctc
     3181 aacaaacgcg gtcatctcgg aaacaaagct ttcggtgtag cgctcgatga aaaaatataa
     3241 tgggaggtcg cgccgcacac tgtgcgcatc agcgataatg gcggtattgg ggtaattgtt
     3301 gccgatgctg atgaacccgg cgctgcccag cacttcgacg cgctggtcat agccatagac
     3361 tgccgcgcga ctgttgctga tcgtgccgat gacgccattg gcatagcgca gcgtcaccac
     3421 cgtgttagcg acatccccga cggaggcaat gccgggttga tcgagcgtgc tggccaccgc
     3481 gctgacctcc tcaacctcgc tgccgatcaa gaagcgcgcc atgtcgaaat cgtggatggt
     3541 catatcgagg aagatgcctc cagagacacg caggtactcc agtgccggcg gcgcgggatc
     3601 gcgactgatc agatgaatca ccagcggttg gccgatttct ccctcctgaa tcgcctttct
     3661 cacgcggcga tagttggcat cgaagcgccg gttgaaaccg atgtgcagtt ttaccccggc
     3721 gcgttccacg gcgcgaagag cagcatcgat ggagggcaaa tcgagcgcga taggcttttc
     3781 gcaaaagatg tgtttgccat agccagctgc ttccttgatg atacgcgcgt gcgtatcggt
     3841 agacgaacaa ataatcaccg cctggatatc tggatgttcc agcacctcct ggtaatgctg
     3901 agtataaacg gggatgtcgt agcgctgcgc gcactccctg gccgattcga cgaacacgtc
     3961 cgccacgatg agcagatcga ccgatgggat acgcgaggcg agatgctcgg cgtgcaggtg
     4021 gccaattcgt ccggcgccga tcaaaccgac cttcaatctc tgtgcaggca tttgatggtt
     4081 catctccctt tccgggcgga agcatgttgc ctctacaggc caatagtttt cagaaagtca
     4141 cggtttcgct gcgaactcgc cgccgggctg cccataccgg gcaggatatc ctgctccacc
     4201 accacccagc cgcgatagct ccttgccctg agctgcccgg caaacgccga gaaagggacg
     4261 ctcccctgac ccaactcaca aaacaggccg tgtccaagtg atgtgaaata gtcccactcc
     4321 tcggcgcgag actgcgcggc aacctgcggc tggagatctt tgaagtggac gtgccagacg
     4381 cgctcgccga aacgctcgaa gccctccaga actactgcgc catcgacacg gccagaacca
     4441 aagagatagt ggccggtatc gaaaaccagt ccgagcagtt ctggatcggt catatcgagc
     4501 aagcgcgcaa tctcctccgg cgtctcgacg tagccggcgc agtgatggtg gaaagctgtc
     4561 ttcaggccag tctgctccct taccgagcga gcgatacgtt ccgcgccgcc cgcgaaggtc
     4621 cgccactcgg cctggctgag gcccatctgc ggcatcacgc gcccggcgtg gcgagtgcga
     4681 agcgggtcag cgccgttgtc atccgccaga atgaggaacg gcaattgatc gccttgcgcc
     4741 gcgcctgtcg ctgcttccac gagcaggtga gccactttta atgcctgcgc ctccccggca
     4801 gcgtgagcat cctcgcgcct gagcgcgacc ggcacaaagg cgccgaccag ggccagccgc
     4861 cgccgctcca gttcggccct gagcgccgcc gcttcggttg gcatgtagcc ccagtcgcca
     4921 agctcgctgc cggtatagcc ggtgtcgcgc aattcatcga gcatccggct ataggggacc
     4981 tcctcgccct gcaaaccctc gaactccagc gcaccccacg agcagggcgc attggcgaca
     5041 cggattgttg aaacgatctc tatcataatg ttttgctaaa acttgcgcga ccattctttc
     5101 ggccagcgtt caacgacaac cttcttctgc gtatagaatt cgacggcatc gcgtccctgg
     5161 gcgtgcatgt cgccgaagaa gctctctttc cagccggaga aggggaagaa ggccatcggc
     5221 gcggccacgc caatattgat gccgatattg cccgcctgtg cctcgtagcg gaacttcctg
     5281 gctgccgcgc cactgctcgt gaacaggcag gccatattgc catacgcggt gacattgacg
     5341 agttcaatcg cttcatcgat ggtttgcgtg tgcatcaggc ttagcacggg gccgaagatc
     5401 tccgtgcggg cgagttcgcc gcgcgggtca acgtcctcaa gaatggtcgg acgcacaaag
     5461 tagcctcgtt catagcctgc aatcgacgcg ccacgtccat cgacgagtac cttcgcgccc
     5521 tcgcctgtcc ctttcgcgat gagcgattcg atgcgctgct tgctttccga ggtgataacc
     5581 ggccccatct caaccccggc atccagccca tagccgacgt t
//
LOCUS       DASZAK010000287         3534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_78779, whole genome shotgun sequence.
ACCESSION   DASZAK010000287 DASZAK010000000
VERSION     DASZAK010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3534
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78779"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..466
                     /locus_tag="VGT44_05045"
     CDS             44..466
                     /locus_tag="VGT44_05045"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2580199.1"
                     /translation="MSVEENKTLIRRFYEEVFNKRNLAALDDFFAPDHVDHTLPPGLP
                     VGPEGTKQAIATMLKGFPDLRVTIEDMIAEGDKVVIWFTTHGTQQGTLGGIPPTGKQV
                     AVSTIEITRMAGGKIAEDWGLDDRLGMLQQLGLVPAMG"
     gene            complement(463..1668)
                     /locus_tag="VGT44_05050"
     CDS             complement(463..1668)
                     /locus_tag="VGT44_05050"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV2580200.1"
                     /translation="MTNHFQKIAPIQTTEQADPHQGPLAGILVVDLSRVLAGPYCTMM
                     LGDLGATVIKIEHPGEGDDTRRFGPPSVAGESAYYLGLNRNKYGITLDFGTPEGKEQL
                     LKLIKEADVLVQNFRPGSLDRKGLGYEALHALNPGLIYCSISGYGHTGPRRDLPGYDY
                     IAQAESGMMSITGEIDGEPMPVGSPIEDVSTGMYACISILAALRSRDQTGRGQHIDLS
                     LFECAVTMLANIASNTLISGDEATRHGNAHANIVPYQSFHTRDGFIAVACGNDGLFRG
                     LCRLLERGDLAQDPRFTTNSLRLSHREELIGILQEIFLERDTADWLTSLRGAGIPSAP
                     INTVRQALRDEQLTARGLVWECDHPTAGKIQLVGSPMHFSETPTRLYKAPPLLGEDNA
                     TILEEKPLP"
     gene            complement(1670..>3534)
                     /locus_tag="VGT44_05055"
     CDS             complement(1670..>3534)
                     /locus_tag="VGT44_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV2580201.1"
                     /translation="NWLLLAVVLLGGIPLLWETLRQFARKEFSVDTIAILAIAGSLWL
                     GEYLAGAFVVLMLSGGEALEAYALRRARSSLTALADRAPRRAHIWRGDELVSIPAEQV
                     EVGMEIVVKPGELLPVDGVVTSGTSSVSEADLTGEPVPVRKTSGMLVLSGSVNLDGVL
                     QIRASKRSAESKYAQIVRLVEEAQTQKAPIHRLADRYSIGFTIAAVGMAGLAWLLSGD
                     SVYALAVMVVATPCPLILATPIAIMSGIDLAARNGIIVKSGASIEQLGEVDIAVFDKT
                     GTLTLGTPKVTAIVLVGHDTPGDEPEKVNESCENDGASYDEDTLLRFAASVEQLSTHI
                     LARAVVEAAQERALPLSPACDFEEMFGKGVHGRVPTLPEEQQAERHSAQDVEVAVGNR
                     TFMQQVGVTLPLSLLSERERRVEEGQICSFIAVEKRAQGLLVLEDVPRAELARLSSDL
                     KAAGIKQTIMLTGDSEVVARQIGKIAQVDRVVSRCLPEDKVRIVQELTQQGHRVLMVG
                     DGINDAPALATATVGMALGTQGLTAAATAADTVLLSTDILRVARGVRLGRRVMRVALE
                     GIWVGMGLSVIAMGFAAAGFIVPAAGAILQEAIDVIVILNALRAGRITFQDTWS"
BASE COUNT          708 a         1094 c         1044 g          688 t
ORIGIN      
        1 aatcttaatg aaggaagcaa cacacataaa ggagaacatg gttatgtcag tagaagagaa
       61 caagaccttg atacgccgtt tttacgagga agtgttcaac aagaggaatc tggccgccct
      121 cgatgacttc ttcgcccccg atcacgtcga tcacacactc cctcctgggc tgccagtcgg
      181 tcccgaggga accaaacaag ccatcgccac gatgctgaaa ggtttccctg atctgcgcgt
      241 caccattgag gacatgatcg cggaagggga caaggtggtc atctggttta cgactcacgg
      301 cacacagcaa ggaacacttg ggggtattcc cccaaccggg aagcaagtgg cggtctcgac
      361 catcgaaatc acccgcatgg caggcggcaa aatcgcggaa gactggggcc tcgatgaccg
      421 gctcggcatg cttcaacaac tcggcctggt ccctgcgatg ggctaaggca agggcttctc
      481 ttccaggatg gtcgcattat cctcgcccag caggggcggc gctttataca ggcgcgttgg
      541 cgtctcggag aagtgcatgg gcgaaccgac cagctggatc tttccggcgg tgggatggtc
      601 gcattcccag accagcccgc gcgccgtcaa ttgctcatcg cgcaatgcct ggcgcaccgt
      661 gttgatgggc gcgctgggga tgcccgcgcc gcgcaagctg gtcagccagt cggcggtatc
      721 gcgctcaaga aagatttcct gcaagatgcc gatcagttcc tcacgatgac tgagccgcag
      781 cgaattggtg gtgaagcgcg ggtcctgcgc caggtcgccg cgttccagca ggcggcacag
      841 tccccggaac aacccatcgt tgccgcaggc gaccgcaata aacccgtcgc gtgtgtggaa
      901 cgattgatag gggacgatat tagcatgggc gttgccgtgt cgtgtggctt cgtcgccgga
      961 aatcagggtg ttcgaggcaa tgttcgccag catggtcacg gcgcactcaa agagcgaaag
     1021 gtcgatgtgc tgaccccggc ctgtttgatc gcgcgagcgc agggcggcca ggatggagat
     1081 gcaggcgtac atgcctgtcg agacatcctc gatgggcgaa cccaccggca tcggttcgcc
     1141 atcgatctcg ccggtaatcg acatcatgcc ggactcggct tgcgcgatat aatcatagcc
     1201 gggcagatcg cggcgcgggc cggtgtgtcc gtatccactg atcgagcaat agatcaggcc
     1261 gggattgagg gcgtgcaggg cctcgtagcc cagtcctttg cgatcgagcg aaccggggcg
     1321 aaaattttgc accaggacat cagcctcttt aatgagtttt aataattgct cttttccctc
     1381 gggcgtgccg aaatcgaggg taataccata tttgttgcga ttcagcccca ggtaataggc
     1441 gctctccccg gcaacagagg gcgggccgaa tcgccgcgtg tcgtcgcctt ctcccggatg
     1501 ctcgatcttg atgacggtcg cgcccaggtc gcccagcatc atggtgcagt acggcccggc
     1561 caggacgcgc gaaagatcaa ccactagaat gcctgccaat gggccctggt gcgggtccgc
     1621 ctgttctgtt gtttgtatcg gcgcaatttt ctgaaaatga ttggtcatat taactccatg
     1681 tatcctgaaa tgtgatccta cctgctcgca gagcattcag gatcacaatc acatcgatag
     1741 cttcctgtaa aatggctccg gcggcaggaa cgataaatcc ggcggcagca aagcccatcg
     1801 cgatcacgct caagcccatc cccacccaga taccttctaa ggctacgcgc atcaccctgc
     1861 gacccaggcg caccccgcgc gcgacgcgca ggatatcggt cgaaagtagc actgtatcgg
     1921 cggcggtcgc ggcagctgtc aatccttgcg ttcccagcgc catgccgacc gtcgcggtcg
     1981 ccagcgcggg cgcgtcgttg atcccatcgc ctaccatcaa cacgcgatgc ccctgctgcg
     2041 tgagttcctg cacgatgcgc actttgtcct ccggcaggca gcgcgagacc acgcgatcaa
     2101 cctgggcgat cttgccgatc tggcgagcca ctacctcgct atcgccggtg agcatgatgg
     2161 tttgcttgat gcccgccgct ttcaagtcgc ttgagagacg cgccagttcc gcgcgcggca
     2221 catcttcgag tacaagcaag ccctgcgctc gcttctccac cgcgataaag ctacagattt
     2281 gcccctcctc gacgcgccgc tcgcgttcgc tgagcagcga gagaggcagg gtgacgccaa
     2341 cctgctgcat gaatgtgcgg ttgccgacgg caacttctac gtcttgtgcg ctgtgccgtt
     2401 ctgcctgctg ctcttcaggg agcgtgggaa cccgtccgtg cacgcctttg ccaaacattt
     2461 cctcgaagtc acaggcagga ctgagcggta gcgcgcgttc ctgtgcagct tctaccaccg
     2521 ctcgagccag gatgtgcgtc gatagctgct caaccgaggc agcgaagcgc aacagggtgt
     2581 cttcgtcata actggcacca tcgttctcac agctctcatt cactttttcc ggttcgtcac
     2641 ccggagtatc gtgtcccacc agcacaatcg ccgtcacttt gggcgtgccg agcgtcaggg
     2701 tgccggtttt atcgaacacg gcgatatcga cttcgcccag ttgttcaatg gacgcgccac
     2761 ttttgacgat gatgccattg cgcgccgcca ggtcgatgcc ggacataatc gctataggcg
     2821 tggccaggat caacggacag ggggtggcga ctaccatcac agccagggcg tagacgctgt
     2881 ctcccgatag aagccatgcc agccccgcca tgccgaccgc cgcgatggta aagccgatgc
     2941 tgtagcggtc ggccaggcgg tggatgggcg ctttctgcgt ctgagcttct tcgaccaggc
     3001 gcacaatctg cgcatatttg ctctcggcgc tgcgcttgct ggcgcgtatc tgaagcacgc
     3061 catccaggtt gacgctgcct gagaggacga gcattccgct cgttttgcgc accggaacgg
     3121 gttcgcctgt caggtcagcc tcgctgacgc tggatgtgcc ggaggtgacg acgccatcga
     3181 cggggagcag ttcgcccggc ttgaccacaa tctccatgcc gacctccacc tgctccgccg
     3241 gaatactgac aagctcatcg ccccgccaga tatgcgcgcg gcgcggcgcg cggtcggcca
     3301 aagcggtgag cgaggaacgc gcgcgccgca gggcataggc ttccagcgcc tcaccgccgg
     3361 aaagcatcaa gaccacgaat gcgcccgcca ggtactctcc cagccagagc gagccagcga
     3421 tggccaggat ggcgattgtg tcaacgctga actcttttcg cgcgaattgc cgcaacgtct
     3481 cccataacag aggaatgccg cccagcagca caacggccag caacagccag ttgg
//
LOCUS       DASZAK010000288         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_220339, whole genome shotgun sequence.
ACCESSION   DASZAK010000288 DASZAK010000000
VERSION     DASZAK010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220339"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..>2113
                     /locus_tag="VGT44_05060"
     CDS             95..>2113
                     /locus_tag="VGT44_05060"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEV2580202.1"
                     /translation="MEKIVLKAGDAFLIANERGDLSASKKETGFFWHGTRFLSECNLL
                     LAGQEPTMLAYTIADDGTSCHIEMMNAVQSLNQGDELLEGTIHVHRLIEVRDDRLVQT
                     ISLTNYHLTQLMLELSLTLDADFVDIFEVRGYTRAARGELFPPVFDQSSTGFQYMGLD
                     AIPRVTRVTVTPLADEVSSGKVSWHIKLSKGQTVQIRATATVGETGVASHPPLSLQGR
                     EVVTSDPPLNQVLMRGMQDLAMLCGATPQGLYPYAGVPWFACPFGRDGLITSLQFLPW
                     FPDVAHDTLSFLAAHQGSKYEAYTEEEPGKILHEFRTGEMANCREIPFSAYYGSIDAT
                     PLFLITLESYIRWSNDLPFLTALWSHAQAAAHWIKDAGDQDGDTFLESRTTSQKGLVN
                     QGWKDSHDSISHANGALAEAPIALCEVQGYAYAAYRAMIYLAGRLGKAEEAAHWSDIA
                     GKLQANFLQRFWWEEESAFYLALDGTKQPCAVVASNAGQCLWTGIVPDDLAQRVIERL
                     LRDDMYSGWGIRTLSTQAARYNPLSYHNGSVWPHDVALVGLGFARYGRKAEASRLLRD
                     LAQAGQYFDRARLPELFCGFSRSTGPGPIPYPVACEPQAWAAGAPFMLLNAALGLEPD
                     AEHHRLLVRQPALPSWLAIVELRGLRLGGQEVSLRYERSGETTASIITR"
BASE COUNT          433 a          616 c          612 g          452 t
ORIGIN      
        1 ggcatcctgg ctggctcagt caaaggataa gcacaggcta ttcagaaggg attttcgacc
       61 cgcttgaata tgagctagaa aataccggtg agcgttggaa aagatcgtac tcaaagcggg
      121 cgatgccttt ctgattgcga atgaacgcgg cgatctgtcc gcgtcgaaaa aggagacggg
      181 ttttttctgg catggaaccc gcttcctgag cgagtgcaat ctattgttgg ctggccagga
      241 gccaacaatg cttgcctata ctatcgccga tgacggaacc tcctgtcaca tcgaaatgat
      301 gaatgccgtg cagtcactca atcaaggcga tgaactactg gaagggacga tccacgttca
      361 tcgcctgatc gaggtccgcg acgaccggct tgtacagacg atcagcctta caaactacca
      421 cctcacccag ctcatgctag agcttagtct gacgttggac gccgatttcg ttgatatctt
      481 cgaggtgcgc gggtatacac gcgctgcgcg cggagagctt ttcccaccgg tatttgacca
      541 atcctcgact ggtttccagt acatgggact tgatgctatc ccccgcgtca cccgcgtgac
      601 agttacaccg ttggccgacg aagtttcttc tggcaaagta tcctggcata tcaagctcag
      661 caaaggccag acagtgcaga ttcgggcgac cgctacggtt ggcgagacag gcgtagcgag
      721 ccacccgcct ctgtcactgc aggggcgtga ggtcgtgaca agcgatcccc ctctcaacca
      781 ggtactcatg cgcgggatgc aggacctggc tatgctatgt ggcgcgacgc cgcagggtct
      841 gtatccctac gcaggcgttc cctggtttgc ctgccctttt ggacgcgatg gcctgattac
      901 tagcctgcaa ttcctgccct ggtttcccga tgtcgcgcat gatacgctgt cctttctcgc
      961 agcacaccag ggaagcaaat atgaggcata cacggaggag gagccgggca aaatcctgca
     1021 cgagttcagg actggcgaga tggcgaactg ccgtgaaata ccttttagcg cctactacgg
     1081 cagcatcgac gctacgccgc tttttctcat cacgcttgag tcctacatac gctggtcaaa
     1141 cgacctgcca tttctcactg ctctctggtc tcatgcacag gcagcagcgc attggataaa
     1201 ggatgctggc gaccaggatg gcgatacatt tctcgagagc cgcacaacct cgcaaaaagg
     1261 actcgtcaac cagggctgga aggactcgca cgattccatt tctcacgcga atggggcgct
     1321 cgccgaggct cctatcgcgc tttgcgaagt gcaggggtat gcctacgctg catatcgcgc
     1381 catgatctac ctggccgggc ggctcggcaa ggcggaggag gctgcccact ggtcggatat
     1441 cgccgggaag ctccaggcga atttcttgca gcgcttctgg tgggaagaag aatctgcctt
     1501 ctacctggca ctggacggca caaagcagcc gtgtgccgtc gtcgcttcaa acgcggggca
     1561 gtgcctatgg acgggcatcg tgccggatga cctggcgcag cgcgtcattg aacggctcct
     1621 gcgtgacgat atgtactcgg gctggggcat acgcacgctt tctacacaag ctgctcgtta
     1681 taatccactt agctatcata atggctctgt ctggccccac gacgtggcgc tggtcggcct
     1741 gggcttcgcg cgctatggcc ggaaagccga agcgagtaga ctgttgcgag acctggccca
     1801 ggccggccag tacttcgatc gcgcgcgcct tccagaactg ttctgtggct tctcccgttc
     1861 caccgggcct ggtcccattc cctatcccgt cgcttgcgag ccgcaagcct gggcagcggg
     1921 cgcgccgttt atgctattaa acgccgcgct gggtcttgaa ccagatgccg aacatcaccg
     1981 attgctggtg cgccaaccgg ccttgccgtc ctggctggca atcgtggaat tgcgcggtct
     2041 gcgcctggga gggcaagagg tgagtctgcg gtacgagcga tcaggtgaaa cgaccgcctc
     2101 gatcatcacg cga
//
LOCUS       DASZAK010000289         4326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_149371, whole genome shotgun sequence.
ACCESSION   DASZAK010000289 DASZAK010000000
VERSION     DASZAK010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149371"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            243..905
                     /locus_tag="VGT44_05065"
     CDS             243..905
                     /locus_tag="VGT44_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV2580203.1"
                     /translation="MAQSTIHAVIWDLDGVIIDSADEHRRAWHRLAQEEEIPFTDIDF
                     WATFGKRNNDIIPQFWGDISAQQVADLANRKEVHFREYIRDAATPLPGSMELMRDLQK
                     AGYRQALASSTPVENIRLISEVLHLERYLSKLVSGESVARGKPAPDIFLKAAAELDAL
                     PTHSLVIEDAVAGILAARAAGMHCIAVAGDRDLPGLRAADRVVRDLTEVSVEQVNSLL
                     KI"
     gene            complement(915..1778)
                     /locus_tag="VGT44_05070"
     CDS             complement(915..1778)
                     /locus_tag="VGT44_05070"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEV2580204.1"
                     /translation="MTAVSIDGRAMGAQIKAELQQEVERYVQQNGKAPGLVIVMVGGD
                     AASGVYSKAILRAAQNIGLDARVEQLPTLTTSDELRALLLSLNHDAATKGIIVQMPLP
                     GHLSQKMVADTIDPAKDIDGISPRSAGNLFLGLPSFLPATAAAVMEMLERMRTPLEGQ
                     RAVVISRSNVVGKPLAMMLMQKNATVTVCHSRTVNLPAYTRQADVLVVAAGRAQMITG
                     EMVRPGAVVIDVGIHETPQGGIVGDVDFASVQQVAGAVTPTPGGVGPLTNIILLKQCV
                     SAAWQQAGNCP"
     gene            complement(1775..2398)
                     /locus_tag="VGT44_05075"
     CDS             complement(1775..2398)
                     /locus_tag="VGT44_05075"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEV2580205.1"
                     /translation="MYIEQPLRHYLDELASGQPTPGGGSTSALSGAMAAALASMVARL
                     TLGKAGYEEAQAEIEVIITQSEEERQRFGELLEEDIAAYGRLSESYKLPRGTEEERAA
                     RSSAIQEHLAGAALVPLEVVETAARLSPLFARIAEIGNAGILSDLETAVMLANTAARG
                     AAGMVRVNLRYMRDAHLVSQLQTRLDAALQQVEESGKQAIEIVGSRA"
     gene            complement(2431..3120)
                     /gene="nth"
                     /locus_tag="VGT44_05080"
     CDS             complement(2431..3120)
                     /gene="nth"
                     /locus_tag="VGT44_05080"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV2580206.1"
                     /translation="MSIATSIHLTHIYDLLIATYGHPVNRPDYDPVGGLVGTILSQHT
                     SDINSDRAYQQLVAMFPMWEAVRDAPTYEVAEAIRSGGLANIKARRIQDVLHVLSEQQ
                     IARGDTHPLASALDNLVGMKPEQAWRLLRELPGVGPKTAACVLMFNLNLPVMPIDTHV
                     HRVSQRLGLIGPKVSADQAHEIFAGIVPPEWVYPLHVNLIRHGRQVCHAQRPECSRCA
                     LYSECAFVGGV"
     gene            3233..4294
                     /locus_tag="VGT44_05085"
     CDS             3233..4294
                     /locus_tag="VGT44_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEV2580207.1"
                     /translation="MIDLRSDTVTLPTPAMREAMYRAEVGDDVYKEDPTINRLQEMAT
                     ERLGKEAALFVASGTMGNAAALLVHAGRGEAVMMGDQSHIYRYEVGGASTLGGSPMIV
                     LPTEPGGMLNPRQVEINYADGSDEHSAPAVLLCIENTHNRCGGTVLSLEQMVTLATLA
                     HERGMQVHLDGARIFNAAVALGIPVGELVRSVDSVMFCLSKGLSAPVGSLLVGSRDFI
                     KRAHRVRKALGGGMRQAGVLAAAGIVALEQMVERMAEDHEHSKQLARGLADYPQIDVT
                     VERVVTNMVIFSVRNAQQEPLSEAETESFLEKAREHGVLMGQIGEGKIRAVTHYGIEA
                     NDIEAALVGIRRALIAMRL"
BASE COUNT          886 a         1255 c         1291 g          894 t
ORIGIN      
        1 gtattgtacc atccttattg ttgggcgtct acctatttga gcgagtgcag gcaatatata
       61 cagtccaggc atagtgaccg caagggctca ccactacgat aacgtcggtg ttcgttcatc
      121 cgtcgttcgt tatcgtagtg gcgatccctt gcggtcgcca tgttgtccaa cgaactcgtc
      181 acgcgccgtg aggggaatgc catgtgatat gattcggcaa actacaaagg agcattgacc
      241 tgatggcaca atccactata cacgccgtga tctgggatct cgacggcgta attatcgata
      301 gcgcggacga acatcggcgc gcctggcacc gattggcgca ggaagaagag atacccttca
      361 ctgatatcga tttctgggcc acttttggta aacgcaacaa tgatattatt ccgcaattct
      421 ggggcgacat ttcagcacag caggttgccg atctggccaa ccgcaaagaa gtccatttcc
      481 gtgaatatat ccgcgacgca gcaacgccct tgccaggttc gatggaattg atgcgcgatc
      541 tgcaaaaggc tggctaccgg caggcgctcg cctcttccac gcccgtcgaa aacatccgac
      601 tcatcagcga agtgctgcat ctcgaacgct acctttccaa actcgtctca ggcgaatccg
      661 tcgcgcgcgg caaaccagcg cccgacatct tcttgaaagc cgccgccgaa ctggacgcgc
      721 ttccaacgca ctcgctggtg atcgaagatg ccgttgcggg catcctggcc gcgcgcgccg
      781 ccggaatgca ctgcatcgcc gttgctgggg accgcgacct gcccggcctg cgcgccgccg
      841 accgggtagt gcgggatctg acggaggtga gtgtcgagca ggtgaatagt ctacttaaaa
      901 tatgaggatc gcatttacgg acagttgcct gcctgctgcc atgccgcgct gacgcattgt
      961 ttgagcaaga tgatattcgt gagcgggcca acaccgccag gagtgggcgt gactgccccg
     1021 gcgacttgtt gaaccgaggc gaaatcgaca tcgccgacga taccaccttg cggcgtctcg
     1081 tggataccga catcgatcac aaccgcgccg ggacgcacca tctcacccgt aatcatttgc
     1141 gcgcgaccgg ctgccacgac cagcacatcg gcctggcgcg tgtaagcagg caggttgacg
     1201 gtgcgtgaat ggcacacggt caccgtggca ttcttctgca tcagcatcat cgccagaggt
     1261 ttgcctacca cgttgctgcg cgaaatgacg accgcccgtt ggccttcgag cggtgtgcgc
     1321 atgcgttcga gcatctccat caccgccgct gctgttgccg ggagaaagct gggaaggcca
     1381 agaaagaggt tgcccgcgct gcgcggactg ataccgtcga tgtcttttgc aggatcaata
     1441 gtatcggcga ccattttctg cgagagatgg ccgggcagcg gcatctgcac aatgatgcct
     1501 ttcgtcgcgg cgtcgtgatt gagcgaaagc agcaacgcgc gcagttcatc ggacgttgtc
     1561 agcgtgggaa gttgttccac acgagcgtca aggccaatgt tctgggcagc gcgcaggatc
     1621 gctttgctat acacgcccga tgctgcatcg ccgccaacca tgacaattac cagccccggc
     1681 gctttaccat tttgctgaac gtagcgctca acctcctgct gtaattccgc cttgatttgc
     1741 gcacccatgg cgcgcccatc gatactgacc gccgtcacgc tctactccca acgatttcga
     1801 tcgcctgctt accgctttcc tcgacctgtt gcagggcggc atccagcctc gtctgtaact
     1861 ggctgacaag gtgtgcatcg cgcatgtaac gcaggttgac gcgcaccatg cctgccgcgc
     1921 ccctggctgc cgtgttcgcc agcattaccg ccgtctcaag gtcgctcaaa atgccagcat
     1981 tgccaatttc ggcgattcgc gcaaataatg gcgagagacg cgcggcggtt tccacaacct
     2041 cgagtggcac aagcgctgcg cccgccaggt gttcctggat cgcgctggag cgagccgcgc
     2101 gctcttcctc cgtaccacgt ggcaatttgt aagattctga caatcgccca taggccgcga
     2161 tatcttcttc aagtagttca ccgaagcgct gccgctcctc ttcggattgt gtaatgataa
     2221 cctctatctc agcctgtgcc tcttcgtaac ccgccttgcc aagcgtcagg cgagcgacca
     2281 tactggccaa cgccgccgcc atcgcgccgc tcaacgccga ggtcgagccg ccaccgggag
     2341 taggttgacc ggaagccagc tcgtcgagat agtgccgaag tggttgttcg atatacatgc
     2401 atcttccttt ccattttcca cgctattgta tcacacaccg cccacgaagg cgcattcgct
     2461 atacagtgca cagcgcgaac actccggcct ctgcgcatgg caaacctgcc gtccatgccg
     2521 gatcagattg acgtgcagtg gatagaccca ttccggcggc acgattcccg cgaagatttc
     2581 gtgtgcctgg tccgcgctca ccttcggccc gatcaacccc aggcgttggc tgacgcggtg
     2641 aacgtgggta tcgattggca taacgggcaa atttagattg aacatgagga cacaggccgc
     2701 cgtcttcggt cccacgccgg gcagctcgcg cagcaatcgc catgcttgct ccggtttcat
     2761 ccccacgaga ttatcgagcg cactggccaa cgggtgcgta tcccctcgcg cgatctgctg
     2821 ttcgctcaaa acatgcagca catcctgaat gcgacgcgcc ttgatattcg ccaaccctcc
     2881 gctgcgaatg gcctcggcaa cctcatatgt gggcgcatcg cgcaccgcct cccacatggg
     2941 aaacatggcg acaagctgct ggtaggcccg gtcggagttg atatcggagg tatgctgcga
     3001 gaggatggtg ccgacgagtc cgccaacagg atcatagtcg ggccggttga ccgggtggcc
     3061 ataggtggcg ataagcaggt cgtaaatgtg agtaagatga atagaggttg caatggacat
     3121 ttcgatgatc tatcctacat ttcgttcaag gttcaatagc cgtgtacatg atatcataag
     3181 gcaggtaaag attattgcca ctcactttga attcgtaaaa ggagtctcaa ccatgattga
     3241 cttacgcagc gacaccgtca cgctacccac gcccgccatg cgcgaggcga tgtatcgcgc
     3301 ggaggtgggc gatgatgttt ataaagagga tccgaccatc aatcgcttgc aggagatggc
     3361 gacggaacgt ttggggaagg aggcggcgct gtttgtggcg agtggcacga tgggcaatgc
     3421 tgccgcgctg ctggtccacg ccgggcgggg cgaggccgtg atgatggggg atcagtcgca
     3481 tatttatcgc tacgaggtgg gcggagcttc gacactgggc gggtcgccca tgatcgtgct
     3541 accgaccgag ccgggtggca tgttgaaccc acgccaggtt gagatcaact atgccgatgg
     3601 aagcgatgag catagcgcgc ctgcggtgct gttgtgtatc gagaatacgc ataatcgctg
     3661 cggcgggacg gtattgagcc tggagcagat ggtgacgctg gcgacactgg cgcatgaacg
     3721 cgggatgcag gttcacctgg atggcgcacg catcttcaat gccgccgtag cactgggcat
     3781 tcctgtaggc gaactggtca ggtcggtcga ttctgtgatg ttctgcctgt cgaaggggct
     3841 gagcgcgccg gtcggctcgc tgctggtggg cagccgcgac tttatcaagc gcgcgcaccg
     3901 cgtgcgcaag gcgctgggcg gcgggatgcg ccaggcgggc gtgttggcgg cggctggcat
     3961 cgtggcactg gagcagatgg tagagcgcat ggccgaagac cacgaacata gcaagcaact
     4021 ggcgcgcgga ttggcggatt acccgcagat cgacgtgacc gtcgagcgcg tcgtgaccaa
     4081 catggtcatc ttctccgtgc gaaacgcgca gcaggagccg ttgagcgagg cggaaacgga
     4141 gagcttcctg gagaaggcac gggaacacgg cgtattgatg gggcaaatcg gcgagggcaa
     4201 gattcgcgcc gtgacgcact acggcatcga ggcaaatgat atcgaggccg cgctggtcgg
     4261 cattcgccgg gcgttgatag cgatgcgttt gtgactttcg tcatagctgg aattatgact
     4321 tgttgc
//
LOCUS       DASZAK010000290         6176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_149415, whole genome shotgun sequence.
ACCESSION   DASZAK010000290 DASZAK010000000
VERSION     DASZAK010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149415"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2660
                     /locus_tag="VGT44_05090"
     CDS             <1..2660
                     /locus_tag="VGT44_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580208.1"
                     /translation="ATVAGLDSAAFTTTVPFAAQGSDSAPIELTFTGLDAEGAGAFAH
                     HELGGGAVGFKNGRIRITGRIIPIGPVTPGGSADPFDDVKNVDVILVQEQTPPNVDVV
                     LNRSAFSYDEITLTNTGGFSPFANAFFVVLQDPPQPPSSIDAVNIFADPSHTAVAGIF
                     ADASFQPTITVVDETGATVDWFIPLFTDVFQEDPTLPAHVSQRVLFRYQMLVNVSAID
                     ALLPAPGAAPHFARLKISARDRVNNQVHDVFSPPIKFFRDAHPYMIDVIGTNPSWLSV
                     DTRVFSVTQNEMKFGHSVSMSGSPTQYIHDVINEFNAGTQDFNSIPADENQAPLELLP
                     QVHGSNVYDFALARVRMKTQVAVNDVHVFFRLFTTAISDLSFTPSNYPTSPGASPIAL
                     LGRTPAGSEIVSIPFFADPRIETRAGATGASSMTSQTDGPNVQAFPITLPGVGQESIR
                     YFGAYLDINSDVPRYPAAPAGDGPFPSAECVSIRNILRGQHQCMVAEVFYSPDPTQPN
                     STPATSDHLAQRNLLIIETDNPGTAATHTVQHSFDIVLSIQEFEQSRATMLVANHSTL
                     MSVKGRDFPLFDELVFFWNNLPPESEVLISLPSMLSEYVLLLRNLRGAPNTVRFVDEH
                     TLALTPAGVTYLPIPFIGSERIAGLLTVTLPDTVKRGEVYTVDVMQIRPAQELGLGGF
                     RLTIPVRKAAQLFERELGLLQVFEERLACTPPTNRWYPIMLEQVDYQRKRALGMAGEA
                     ADECSDQEDGVRIRVLLERIDVLDAYGPLVHGSGQLRLLALVTSNDGGGVGSMTNLPK
                     TGFYTVKDQDGGYRIDINREIFRGTVTDDLTIELDSAETGYMARTSPYIRKFSGSALT
                     WLGSYNPGDEQYDPEDVGDWKVWYRIEKL"
     gene            complement(3092..4228)
                     /locus_tag="VGT44_05095"
     CDS             complement(3092..4228)
                     /locus_tag="VGT44_05095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2580209.1"
                     /translation="MRWRMSFKASLGGKFLLSYLLVVLVGVATLLVVTFALAPTLFQA
                     QLMPILQTHPNTLTTAEATQRILGDFLGTLFAALAVAAIVATVTSLAISLFVARRITG
                     PLRQMTQASERISAGHYAERIEIAPVHATDELGQLASSINALAVALEQTERRRLEVIG
                     NVTHELRTPIATLEGYLEGLLDGVIEPTPRTWAMLHTEAGRLRRLVDDLQELSRAEAR
                     QIPFSLQSVAPQRLVQDALEPLEGQFAEKGLDLQTYLPQDLPPVKADPTRAVQILTNV
                     LVNALRYTPAPGRVEVAVSREEGVVAFKVSDTGTGLSPEQLAHIFERFYRVDKSRSRA
                     FGGSGIGLTIAQSLAQAMGGEIRVESAGLGQGSSFTLTLPQAHA"
     gene            complement(4233..4949)
                     /locus_tag="VGT44_05100"
     CDS             complement(4233..4949)
                     /locus_tag="VGT44_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580210.1"
                     /translation="MTRILVVDDETALVELVRSYLEREQYEVLTAADGRTALDLARTA
                     QPDLVVLDVMLPVLDGIEVCRQLRQFSDAYVIMLTARAEEIDKILGLTVGADDYLTKP
                     FSPRELVARIKALLRRPRQTATALQSDSEPPDEAPAPQQWGDLTIDEAQHEVTLHGQP
                     VELTAREFALLLALAQHPGRVFTRAQLLERVWGDAYYDDHVVDVHIGNLRKKLEADPA
                     HPQYLETVRGVGYRFRRRRE"
     gene            5528..>6176
                     /locus_tag="VGT44_05105"
     CDS             5528..>6176
                     /locus_tag="VGT44_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580211.1"
                     /translation="MRLYPKASAYLRWWLDHRRDAEGWMVYAFSWESGQDVSSRFGPQ
                     QTGGTIIQHVRPVDLQASVALSAEILSRWATILAGNQDTADAPALPWQAGDLTSDATW
                     WREIAGEFSVKTRTMWRDGWFRDYDSRSREWSTQQDAMHLAPVFCGVVGGDYIEHLRP
                     YLAKPPIHSSGWAPLSWPPVVMTLVGAASAAQMPQDAAELAYRFIDASYRSSDRRE"
BASE COUNT         1342 a         1705 c         1754 g         1375 t
ORIGIN      
        1 tggcaaccgt tgccgggttg gatagcgccg catttaccac gactgtcccg ttcgctgcgc
       61 aaggcagcga ctctgccccc atcgaactga ccttcaccgg tctggatgct gaaggtgccg
      121 gggcttttgc gcaccatgaa ctgggtggtg gtgcggttgg tttcaaaaat gggcgcattc
      181 gcatcactgg acgcatcatt cctatcggtc ccgtcactcc aggtggttca gcagacccgt
      241 ttgatgatgt gaaaaatgtc gatgtgatcc tcgttcagga gcaaacgccg cccaatgttg
      301 atgtagtact caatcggagt gccttctcct atgatgaaat cacccttacg aacactggcg
      361 gcttcagtcc tttcgccaat gcatttttcg tggtcttgca ggatcctcca cagccgccct
      421 cgtcgatcga tgcggtgaac atctttgctg acccatcgca tacagcagtg gccggtatat
      481 tcgctgatgc aagctttcag ccaacgatca cggtagtgga tgagactggg gcaactgtcg
      541 actggtttat acccctgttc actgatgtgt tccaagaaga cccgactttg cctgctcatg
      601 tctcgcaacg ggtgctgttc cgctaccaga tgctcgtcaa tgtctcggct attgatgccc
      661 tgctaccagc tcctggcgcg gcaccacact ttgcccggtt aaagatcagc gcgcgagatc
      721 gcgtcaacaa ccaggtgcat gatgtgttca gtccgccgat caagttcttt cgtgacgccc
      781 atccttacat gatcgatgtg atcggaacca acccatcatg gttgagcgtg gatactcgcg
      841 ttttctctgt aacacagaac gagatgaagt ttgggcattc ggtatccatg tccggcagtc
      901 caacccagta catccacgat gtcatcaatg aattcaatgc tggtactcag gatttcaact
      961 ctatccctgc cgatgagaac caggcacccc tggaactgct gccgcaggta catggaagta
     1021 acgtctacga ctttgccctg gcacgggtgc gcatgaagac gcaggtggcg gtcaatgatg
     1081 ttcacgtgtt tttccgcctc ttcacaacgg ccatcagcga tttgagcttt acgccgtcga
     1141 actaccctac ctctcctggc gcgtcgccca tcgcattgct gggccgcacc cccgccgggt
     1201 cggaaatcgt ctctatcccc ttcttcgcgg atccacgcat agagacccgt gcgggagcaa
     1261 cgggtgcaag ttccatgacg agtcagacag atgggccgaa tgtacaggca ttcccaatca
     1321 cactaccggg agtggggcag gagagcattc gctacttcgg tgcttacctg gacatcaact
     1381 cagatgtgcc gcgttatcca gcggcaccgg cgggcgacgg gccattcccc agtgcagaat
     1441 gtgtctcgat tcgcaacatc ttgcgcggcc aacatcaatg catggtcgcg gaggttttct
     1501 atagtcctga ccccacccag ccaaactcga ctccggcaac ctctgaccac ctggcccagc
     1561 gcaatttgtt gatcattgag actgataatc ctggcaccgc ggcaacccat accgtgcaac
     1621 atagcttcga tatcgtgttg tccatacagg agtttgagca atcgcgagca accatgctgg
     1681 tagccaatca ttccacgctg atgtcggtta aagggagaga ctttcccctg tttgacgagt
     1741 tggtcttttt ctggaacaat ttaccgcctg agtccgaggt gctgatctct ctaccgtcga
     1801 tgttgagcga atacgttctg ttgctacgca atttgcgagg cgctcccaat acggtgcgtt
     1861 tcgtagatga gcacacactg gcattgacgc ctgccggtgt tacctacctt cccatcccct
     1921 tcattgggag cgagcgaata gctggactgc tgaccgtgac tctacccgat acggttaaaa
     1981 gaggcgaggt gtacaccgtt gatgtcatgc aaatacgtcc cgcgcaggaa ctgggtctgg
     2041 gcggatttcg cctgacgatt cccgtgagga aggcggcaca gttgtttgag cgtgagttag
     2101 ggctgttgca ggtctttgaa gagcggctag cctgtacgcc gccgaccaat cgctggtatc
     2161 ctatcatgct ggaacaggta gattaccagc gtaagagagc actgggtatg gctggggagg
     2221 cggcggatga gtgttccgac caggaagatg gcgtgcgcat tcgcgttctc ctggaacgta
     2281 tcgatgtgct tgatgcctat ggaccactgg tgcatggttc aggccaactg cggctattag
     2341 cgcttgtgac cagcaacgat ggaggtgggg tcggcagcat gaccaacttg ccgaagaccg
     2401 gcttctacac tgtcaaggat caagatggtg gctatcgcat cgatataaac agggagatct
     2461 tccgggggac agtcaccgac gatctcacca ttgaacttga tagcgcggaa acaggctata
     2521 tggcacgcac cagcccctac attcgcaagt tcagcgggtc ggcactaacc tggctgggtt
     2581 cgtacaatcc cggagacgaa cagtacgatc ctgaagatgt cggagattgg aaggtgtggt
     2641 accgcataga aaaattgtaa tcccgatatc aaagggggaa agcagtagtt cgggagcaag
     2701 actggtagaa ccttactaag ctaagagata ttgttggcga atatcgaatt cgccagcaac
     2761 atcataaggg ggtccaatca gaatagcgcc cggaaaaaag taaagccccg gttttctaat
     2821 atcggggctg gctgattcat ccctgctgcc tctatggggt gtttgtgatt actgcgacca
     2881 tgtacaggtg aacttcgcag gtatattgaa tggtacctga tgtgttaaag gtgtagctga
     2941 acgtttgtcc ctggttaagc gggccgctat gtttcatgcc attcttgaac gtaattgtat
     3001 gctgtacgtt taagagtata agctgttcac ttcaagagca catgcgtgtt ttcatcaagt
     3061 ttcgatcaag aaaatgcgtt tgaaaagcat gtcacgcgtg agcctgagga agcgtgaggg
     3121 taaagctgct cccctggccc agcccggcgc tttccaccct gatctcacca cccatggcct
     3181 gggccagcga ttgggcaatc gtcagaccaa tgccggagcc tccaaaggcg cgcgaacgcg
     3241 acttgtcgac gcgatagaag cgttcaaaga tatgggcgag ttgctcgggg gaaaggccgg
     3301 taccggtatc gctgaccttg aatgcaacca cgccttcctc tcggctcacg gccacctcca
     3361 cacgacccgg cgcaggcgta tagcgcagcg cattgaccag tacattcgtg aggatttgca
     3421 cggcgcgcgt cgggtcggcc ttgaccgggg gaaggtcctg cggcagatag gtctgcaggt
     3481 caaggccttt ctcagcgaac tgcccttcaa gaggctcgag cgcatcctgg acgaggcgct
     3541 gtggggcaac agactgcaga gagaagggga tctgacgcgc ctcggcccgc gacaattcct
     3601 gcaaatcgtc taccaatcga cggagtctgc ctgcctcggt gtgcagcatg gcccaggtgc
     3661 gcggagttgg ctcaatcaca ccgtcaagca agccttcgag atatccctca agtgtggcaa
     3721 tcggggtacg cagttcgtgc gtcacattgc caataacctc cagacggcgg cgctccgttt
     3781 gttcgagggc cacagcgagc gcattgatgc tggatgccag ttgcccaagc tcgtcggtcg
     3841 catgcaccgg tgcgatctcg atgcgctcgg catagtggcc cgcgctgatc cgctctgatg
     3901 cctgcgtcat ctgtcgtaag ggcccggtga tccgccgggc gacgaagaga ctgatggcca
     3961 gggaggtcac cgtggcgacg atggcagcca cagccaaagc agcgaagagt gtgccgagaa
     4021 agtcccccag gatgcgctgg gttgcctcgg ccgtggtcaa tgtgttcgga tgtgtctgga
     4081 ggataggcat cagctgggcc tggaacagcg tcggtgccag cgcaaaggtg accaccagca
     4141 aggtagctac accaactaaa acaaccagca ggtaggagag caggaatttg ccgccaaggg
     4201 atgctttgaa tgacatgcgc caacgcataa tactactccc gtctgcgccg gaagcgatag
     4261 ccgaccccgc gcactgtctc gaggtactgg ggatgcgccg ggtccgcttc cagcttcttg
     4321 cgcaagttcc cgatgtgaac atccacgacg tggtcgtcat agtaggcatc gccccagaca
     4381 cgctccagca attgggcacg cgtgaacacc cgaccgggat gctgggcgag cgcgagcaag
     4441 agggcaaact cacgcgcagt caactccacc ggctgcccgt gaagtgtcac ctcatgttgt
     4501 gcctcatcaa tcgtcaggtc tccccactgc tgcggcgcag gtgcctcatc ggggggttct
     4561 gaatccgatt ggagcgcagt cgccgtctga cggggacgcc gcagcagcgc cttgatgcgt
     4621 gccaccagtt cacgtgggct gaacggcttg gtgagatagt catctgcgcc aacggtcagg
     4681 ccgaggattt tgtcgatctc ttcagcacga gcggtgagca tgatcacata ggcatcggag
     4741 aactgccgga gctggcggca gacctcaatg ccgtccagga ccggcagcat gacgtcgagc
     4801 acgacgaggt cgggctgagc tgtgcgcgcg agatcaagcg ctgtgcgccc atctgctgcc
     4861 gtcagtactt cgtactgctc gcgttcaaga taactccgca ctagctccac cagcgccgtt
     4921 tcatcgtcta ccactaaaat gcgagtcaca acaagctcct ttccacagcc gacggatgta
     4981 aggttgctgc tgtgtatggt tagccatagg catgacactc acagcgtcga aagtgagtag
     5041 gtgaagagga gtcaccctcc ctcatactgt agtatatgat actcgctttc caagatgagt
     5101 gctccgcgaa tcactgtgtt acgccgcaga gggaaaagtc tgtagtgcag caggtgaaat
     5161 ttcctcaatg cagttggtgg tgggtattct cttccatgct atgcttccaa ttgccaaaag
     5221 gtgattgctg catcaatcat cagctacata atacaatgta cttgttatcc actgaagtac
     5281 ctttgtatgc gcaaaacaga tgcaagaatg cgggtaccaa gaagttcaac gctaccgagg
     5341 ttcagtgcac agttctctcc cggatgtgcg cttgccaggt gcgggctgac gcacttcaag
     5401 caggccgcgt gccgccaaaa tccgctccag ggcagaaaca tgctcgagct tcggcgggat
     5461 gagaaccgcc tcgccgctgt ggtgctgcga tcagctcttt cgacgcacag gggatagaga
     5521 atggctcatg cgtctctatc ccaaagcttc ggcctatctg cgctggtggt tagatcatcg
     5581 tcgcgatgcc gagggttgga tggtctatgc tttctcatgg gagagcggcc aggatgtctc
     5641 cagcaggttt gggccgcaac aaaccggcgg tacaatcatt caacatgtac gtccggtaga
     5701 tttacaggca agcgtcgcgc tgagtgctga gatactttct cgctgggcta ctatcctggc
     5761 cggaaaccag gataccgcgg atgcaccagc attaccatgg caggcaggtg atctcacctc
     5821 agatgcaacg tggtggcgag agatcgccgg cgagtttagt gtaaagacca ggactatgtg
     5881 gcgcgatggc tggttccggg attatgattc cagatcgcgt gagtggtcga cgcagcaaga
     5941 cgcaatgcac cttgcgcctg ttttttgcgg tgtggttggc ggggactata ttgaacattt
     6001 gcgcccatac ctggcgaagc ctcccataca tagttcgggt tgggctccac tctcctggcc
     6061 tcctgtcgtc atgacactgg taggagcagc ctcagccgca cagatgcccc aggatgccgc
     6121 tgaactcgcc tatcgcttta tcgatgcctc ctaccgcagt agcgatcgtc gcgaac
//
LOCUS       DASZAK010000291         4035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_78907, whole genome shotgun sequence.
ACCESSION   DASZAK010000291 DASZAK010000000
VERSION     DASZAK010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78907"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VGT44_05110"
     CDS             complement(<1..400)
                     /locus_tag="VGT44_05110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2580212.1"
                     /translation="MNEQVRSKAAKTYLLGHTSAALHRLLVQGQMLNPFTRRVFEEVG
                     MTAGMRVLDVGCGPGDVSLIAAALVGETGSVLGIDGSVEALQLARSRTQEASLSQVSF
                     QAGDLLRLTLDQEYDAIVGRLILMHLPEPAA"
     gene            complement(473..1327)
                     /locus_tag="VGT44_05115"
     CDS             complement(473..1327)
                     /locus_tag="VGT44_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2580213.1"
                     /translation="MSNYIFDNAGQQVSQRFTSLETLYDPWTIRHLEATGIGTGWQCW
                     EVGGGGGSIATWLGERCGPTGYALVTDIDPRYLVEIASLDHAHIDIQRHDIGTDSLPA
                     RTFDLIHARLVLIHVPAREAALQRLVMALKPGGWLVVEDFDSTLINSGFPTADAADTV
                     LFQRVAAAQNRLMAARSGELALAWGRSLYRRLRAHGLVDVDMEGYLTVRKGGSPGARL
                     HRANFEQIREEVVNAGFITDEEIAQALTLLDDPDFAIGAPIMFTAWGRRAWPMADVGS
                     AGQPRNAM"
     gene            1662..2060
                     /locus_tag="VGT44_05120"
     CDS             1662..2060
                     /locus_tag="VGT44_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580214.1"
                     /translation="MSTIESQTRDKIVFGLALVIAAEPSKLAQLRQALVLLRAQRYIP
                     EDFSTWAIEQIDARVMSMALENVHDAELLLQILSFKGGSFDRLLCEIEELYRQEKISQ
                     RVYVKAKLLLRAARFDPNDPLHLARHTATE"
     gene            complement(2030..>4035)
                     /locus_tag="VGT44_05125"
     CDS             complement(2030..>4035)
                     /locus_tag="VGT44_05125"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing
                     serine/threonine-protein kinase"
                     /protein_id="HEV2580215.1"
                     /translation="RFREWIQRGVALMVASAHLHCARCGTDNPALAAFCFACGEPFLN
                     AAVTTNSFTGSLAQNHLLKERYRILEQVGKGGFGAVYKAADTLFGYRLVAIKEMEQSR
                     LSPQEMAEAIEAFKREAHLLACLQHPNIPAVYDYFGEAGRWYLVMDFIAGVTLEDYLR
                     TSRGGYLPVEKVLDLGVLLCTVLDYLHTRQPPIIFRDLKPANILVTASGRIYLIDFGI
                     ARHFKPGQVKDTVAFGSPGYAPPEQYGRTQTTPRADVYALGATLHQLLTGDDPTQTPF
                     RFAPLQLRSQTTPVGLDTLVASMLEIDEKRRPASMDTVKRQLQRFVVQRTGAPGGTLQ
                     PGTTYAFSSTAVPRHSFPASQVRTGPASVGSILVEYYGHADRVNAVAWSPDGTQVVSA
                     GDDLTAQVWEFATGDHRFSYTGHTDRVNAVAWSPDGKLVASASSDATVQVWEADSGIY
                     RFSYTGHTDRVHAVAWSPGGTLVASGGDDKTVRLWDATTGHHLFTYRGHSDWVRALAW
                     SPDGRRIASVGDDSTLHVWDAVTRRALFLTRNVYRGASQRIRTVAWSPDGKLVASAGD
                     DKTVHIWIPPSRKPALIYRDHADGLHAVAWSPDGKYLASAAGSSINYYAENTVRIWNA
                     ASGETLYTFRSQSGYIRALAWSPDGQCIASASSDHSVAVWRAR"
BASE COUNT          926 a         1157 c         1112 g          840 t
ORIGIN      
        1 tggcagccgg ttcaggcaga tgcatcaaga tcagcctgcc cacgatcgca tcgtactcct
       61 gatccagcgt caagcgcagg agatccccag cctggaagga aacctggctg agggaagcct
      121 cctgggtacg tgaccgtgcc agctgcagcg cctcaacgct cccatcgatg ccgagcacgc
      181 tgcccgtctc gcctaccaga gcggctgcaa tcagactgac atcgcctgga ccacagccca
      241 catccagcac cctcatccca gcagtcatgc cgacctcctc gaacactcgc cgagtgaacg
      301 gattgagcat ctgtccctgc accagtaacc ggtggagggc tgcagatgtg tgtcccagca
      361 ggtaggtttt ggcggctttg cttcgtacct gttcgttcat ttcactccct cttgctgtct
      421 caaaggtcat tttcaaaggg gccaaattgt ccacgtaaag tattgacaca gactacatcg
      481 cattcctggg ttgcccagcg gaaccaacat cagccatcgg ccaggcccgg cgaccccacg
      541 cggtgaacat gatgggtgcg ccgatggcaa aatcggggtc atcgagcagc gtgagggcct
      601 gtgcgatctc ttcgtcggtg ataaatccgg cgttgacgac ctcctctcga atctgctcga
      661 agttggcgcg atgcaagcgt gcgccgggag acccgccttt ccgcacagtc agatagccct
      721 ccatatcaac atcaacaaga ccatgcgcgc gcagccgccg gtacaggctc cggccccagg
      781 ccagcgccag ttcgccactt cgtgcggcca tcagccgatt ctgtgccgct gccactcgtt
      841 gaaagagcac tgtgtcagcg gcgtccgccg tcggaaaccc ggagttgatc agggtcgaat
      901 caaaatcctc cacaactagc cagcctcctg gcttgagagc cattaccaaa cgttgcaacg
      961 ctgcctcgcg tgcgggtaca tggatcagca ccagccgggc gtggatcaga tcaaaggtcc
     1021 gtgctggcag agagtcggtg ccaatgtcgt gtcgctggat atcgatatga gcgtggtcaa
     1081 gtgacgcgat ttctacgaga tagcgcgggt cgatatcagt gaccagcgca tagccggtgg
     1141 ggccgcagcg ctctccaagc cacgtcgcta tcgacccacc tccaccgccg acttcccagc
     1201 actgccagcc cgtgccaata ccagttgcct caaggtggcg gattgtccag gggtcgtaca
     1261 gcgtctccag gctggtgaaa cgttggctaa cctgctgtcc ggcattatca aagatgtagt
     1321 tgctcatgag tcactctttc atggtttcag agacggtcag ttcatttgct ttttttgaaa
     1381 gtgtacctgc aacaacggcg aactgatatg cgcagcctta cccatttcaa ggcgtgattg
     1441 agaggcgagg acgctgagtg acaataaaat gctaatggca attcatgcga cctgaagtga
     1501 tacctccatg atatctacat gctatccatt tttctctcca tctccggtat agttatcgta
     1561 agcaatgctg cctggctccg gcaggaaata cagaatttcc ggcgagatta gcagtcctcg
     1621 taattatccg taccggattc atgcaaagga ggttggattg tatgtccacc attgagagtc
     1681 agactcgtga taaaatcgtc ttcgggctgg cgctggtgat tgccgctgaa ccatcgaagc
     1741 tggcgcaatt acgccaggcg ctggtacttc tccgggcaca aagatacatc ccagaagact
     1801 tctctacctg ggccattgag caaattgatg cacgagtaat gtcgatggca ctcgaaaatg
     1861 tccatgatgc agaactactt ttgcaaattc tctcgttcaa gggtgggagc tttgacaggc
     1921 tactgtgtga aattgaagag ctgtatcgac aagagaaaat ctctcagcgg gtttatgtca
     1981 aagcgaaact gttgcttaga gcagccagat tcgatcccaa cgatcccctt cacctcgcgc
     2041 gccacacagc caccgagtga tccgatgaag ccgaggcgat acactgtcca tcgggcgacc
     2101 acgccagggc acgtatataa ccagattgac tgcgaaaagt ataaagcgtc tccccgctgg
     2161 cggcattcca gatgcgcacg gtattttccg catagtagtt gatgctgctg cctgccgccg
     2221 aagcaaggta cttgccatcg ggcgaccacg ctacggcgtg cagtccatcc gcgtgatccc
     2281 ggtagattaa ggcgggtttc ctggaaggag ggatccagat atggacagtt ttatcatcac
     2341 cggcagaagc aacgagctta ccatcgggcg accacgccac ggtacgtatg cgttgggaag
     2401 cgcctcgata gacatttctg gtcaagaaca gcgcgcgcct ggttacagca tcccagacat
     2461 gcagcgtgga atcatcgccg accgaggcaa tgcgcctgcc gtcgggcgac caggccagcg
     2521 cgcgcaccca atcggaatga ccgcgatagg tgaaaagatg atgaccggta gtggcatccc
     2581 acaaccttac ggtcttgtca tcaccgcctg acgcgacaag cgtaccgccg ggcgaccacg
     2641 ccaccgcgtg tactcgatcg gtatgacccg tatagctgaa gcgatagatt ccagaatcgg
     2701 cctcccagac ctgtacggtc gcatcactac ttgcggaggc gaccaacttg ccgtcgggcg
     2761 accacgccac ggcattcact cgatcggtat gaccggtata gctgaagcga tgatccccgg
     2821 tagcaaactc ccagacctga gcggtcaggt cgtctccagc agagacgacc tgcgtaccgt
     2881 cgggcgacca cgccacagca ttcactcgat cagcatggcc atagtattcg acaagaatgc
     2941 tccccacgct agcaggcccg gttctgacct gtgaagccgg aaaagagtgt ctgggaacag
     3001 cggtcgaaga gaatgcatat gttgttcctg gttgaagtgt acctcctgga gcgccggttc
     3061 gctggactac gaagcgctgt agctgccgct tgacggtatc catactggcc ggccttctct
     3121 tctcgtctat ttcaagcata cttgcgacca gggtatcgag gccgacggga gtcgtctgac
     3181 tgcgtagctg aagaggcgca aagcgaaatg gtgtttgggt gggatcatcc ccggtaagca
     3241 actggtgaag cgtagcgccc agggcataga cgtccgcgcg cggggtcgtc tgtgttctac
     3301 catattgctc gggaggcgcg taaccaggtg agccgaaagc aaccgtatct ttcacctgcc
     3361 ctggcttgaa gtgccgagcg atcccaaagt caatcaggta gatacgtcct gatgccgtca
     3421 caagaatgtt ggcaggtttg aggtcgcgga agatgatggg aggctggcgc gtgtgcaggt
     3481 agtccaggac ggtgcagagt agcacgccga ggtcaagcac cttctcaaca ggcagataac
     3541 ctcccctgga tgtacgcaag tagtcttcga gcgtcacccc tgcaatgaaa tccatgacaa
     3601 gataccagcg cccggcttcg ccaaaataat catacacggc gggtatattg ggatgttgca
     3661 ggcaggcaag tagatgagct tctcgtttga atgcctcaat agcctcggcc atctcctgtg
     3721 gactcaggcg actctgctcc atctccttga tggcgacgag ccggtagcca aagagcgtgt
     3781 ctgccgcctt atagactgct ccaaagccgc ctttaccgac ttgctcaaga attcggtagc
     3841 gttctttgag caagtgattc tgagcaagcg atccagtaaa tgagttggtc gtgaccgcgg
     3901 cattcaaaaa gggttcccca caggcaaaac aaaacgctgc gagtgccgga ttatctgtgc
     3961 cacacctggc gcaatgaaga tgagcagaag caaccatgag cgctactccg cgctgaatcc
     4021 attcacggaa gcgag
//
LOCUS       DASZAK010000292         6120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_290720, whole genome shotgun sequence.
ACCESSION   DASZAK010000292 DASZAK010000000
VERSION     DASZAK010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290720"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..1588
                     /locus_tag="VGT44_05130"
     CDS             269..1588
                     /locus_tag="VGT44_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2580216.1"
                     /translation="MQETGQETQNKSTAPTRSRKVSWKRKRNPARLPWWRELPVGYLM
                     TFPLVGLGLSVPFGFQYLGITNTFLGTPSVLMTLIIALIWGTEPAILSILISTLALDV
                     YFLSPTGLLSVNSEALLQLVPFVLTQIIIVMVAARLARGRRQILLAQQKLEVNADDLL
                     RVNDKLMQVNQELEKANVLKDHFLSMASHELKTPITTIRGHAQLGMRRLSSLPDLPPE
                     LESLNTAFDRIDQQTFRLNNLVDNLLDLSILRSGKMTLRLSDCDLREICSEVVSEQQS
                     LSGRKIELEIPPSPVVLQADSERLSQVVTNLLTNALKYSPEESPVQVQVSQSKNKAII
                     RVHNDGQPIPKEQLASIFEPFYRAPAAQKSSKKGWGLGLAISQQIVDRHNGRIWVESS
                     EGKGTTFSVELPMKQRIEQAQEMGRSYIPQDRSLAGELLAERRREGE"
     gene            complement(1596..1934)
                     /locus_tag="VGT44_05135"
     CDS             complement(1596..1934)
                     /locus_tag="VGT44_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2580217.1"
                     /translation="MQRYPRDAGLYNNLGVLLANMKHDEQAIRAYEQAIELEPGEVLF
                     YRNLSILLEQLGRGQEAEQLQLRAESAGLQWEETPNPDAPIPEERTIRWEHLPTVHYP
                     MPAILKDLRH"
     gene            complement(1998..2363)
                     /locus_tag="VGT44_05140"
     CDS             complement(1998..2363)
                     /locus_tag="VGT44_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2580218.1"
                     /translation="MSASTIFNEPAPETWAQWINRGHRLQHEEQYADALSAYEQAIIC
                     NPQDASAYEYLAELCKKLGLYESALAVYQRVLGLDPTFPHFYLYKGEVLKKLHRYAEA
                     LTTFEQAMALVPEEVALYD"
     gene            2513..4591
                     /gene="mutL"
                     /locus_tag="VGT44_05145"
     CDS             2513..4591
                     /gene="mutL"
                     /locus_tag="VGT44_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV2580219.1"
                     /translation="MPILQLAPDVAAKIAAGEVVERPASVVKELIENSIDAGATHIRV
                     ELVGGGLSLIHVTDNGGGIPAGELPLALSRHATSKVASIDDLEHIRSLGFRGEALASI
                     AAVADVTLTSRHREEQQGAQVSASNGHISEVMAAASPVGTTMTVRNLFSAVPARLKFL
                     KSRTTEVSHCHHLLEQYALAYPEIRFSVSSEGRQIFATPGDGKISSVLVAIYGLEVAE
                     QMIPVSSLDGRDEDPLRPVATGYISKPTCYKSTRQYMSFFVNRRWVMSRMLSASIEEA
                     YKTLLLTGRHPIAVINIIIDPSQIDVNVHPAKTEIRFLRERRVYSAIVRAVRNAILEG
                     AEMPQWGSASSTAPSSSHDDTSLMRDLPGDLPEDMPAESTNPTGHGNEAFPRDNPWLT
                     VTEEETGTKPPLLSRLWQSHVRGQQALGTEILRFAQDDRTGFDDDMTGFDREHSLSGS
                     TDTSDTSDTNPAHPNSPPRLSNTSDTSDTFAATGSIQASTSMSLLPILRVIGQLSQSY
                     IVTEGPDGMYLVDQHAAHERILLERMVAALQARATLSQLLLTPLRLELAPVELEALEE
                     HLEQLRGIGFTLEILEDGALEARAVPQALLKQINARSLRDLLVELTAPGFDGNNERNG
                     GSQAWQEHALANVACKAAIKANYFLTVSEMRELIEQLGQTGAPFSCCHGRPTMVHFGL
                     SALEREFGRR"
     gene            4592..>6120
                     /locus_tag="VGT44_05150"
     CDS             4592..>6120
                     /locus_tag="VGT44_05150"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2580220.1"
                     /translation="MFAEVLTSAARWQTEQPLLTYIVPVTLEQEARPGQLVAVPYGER
                     LVEGIIWRLTGHLEDTDLPQPADNPRERTSQALTLDQLRPISAILDPEPALLPHQRAL
                     AEWMATYYVTPLAQVAQSMLPPGLAQRSKVVLRLISDDPQPEVEQPGQPSITSTRALI
                     GLLLADGDLDIARLKEMLGPKRAGEVIKEALASGLIAREAQLDQPRARARRKRLVRLR
                     ARGEELEAWKERARTRIQESLPDPTTMPLAPDNVRRRPTKHAPDPWAIPGASSVFTLT
                     RENRVGLLAQRQLAAIDLLEHDRADASAEAHWTPTMLCKASSLHARQLTALAQEGIVT
                     IEELEVQRDPLSGRNIPPSAPLPLTAEQRDALSAIVGDTPILLHGVTGSGKTEVYLQA
                     LAAIIARGQRGIVLVPEIALTAQAIYRFAGRFPGRVAVIHSALSDGERYDEWRRIRAG
                     KVDVVVGSRSALFSPVPDLGLLILDEEHEPAYKQSERKPTYLARDTAIALAKLLHIPV
                     VL"
BASE COUNT         1332 a         1958 c         1641 g         1189 t
ORIGIN      
        1 gaatggatcg gcctgtaagg gcacgttatc gtagtggcga cccttgcggt cgccatgtga
       61 ctgcgatctt acgatcactc agtgactgag accttcctat tacattagat tataaggcat
      121 atacgggcag tagaagcaaa ggttttgagg gggatctgtt cctgttgagc ttgctgttag
      181 tacaagccgt ctgttatatt tgagtaagct tatcaggcta tcatgatccc ccatgatggc
      241 gcgccaatta tcggagaaga atatgtacat gcaagagaca ggccaggaaa ctcaaaataa
      301 atcaactgcg cctactcgat ccaggaaggt gagttggaaa cgcaaacgca atcctgccag
      361 gcttccctgg tggcgcgagc ttcctgttgg ctatctcatg acgttccccc tcgtcgggct
      421 gggactctcg gttccgtttg gtttccaata tttagggata acgaatactt tcctgggtac
      481 accttcagtg ctgatgacac tcatcatcgc gttgatctgg ggtacagagc cagcgatact
      541 ctcaattctt atcagtacac tagctttaga tgtttatttt ctaagtccca ctggccttct
      601 cagtgtcaat tcggaagcat tgcttcagct cgttcctttc gtcctcactc aaattatcat
      661 cgtcatggtc gctgctcggc tcgcgcgagg tcggcggcag attctgttag cgcagcagaa
      721 gctagaagtc aacgccgatg atctcctgcg ggtcaacgat aaactgatgc aggtcaacca
      781 ggagcttgag aaagcgaatg tactcaagga tcattttctt tcgatggcat cgcacgaact
      841 caaaacccct atcaccacta ttcgcggcca tgcccagctt ggcatgcgcc gcctctcatc
      901 actgcccgac ctgccgcccg aactcgagtc gctcaatacc gctttcgatc gtatcgacca
      961 gcaaactttc cgcctgaata acctggttga taatctcctg gatttgagca tcctgcgctc
     1021 aggcaagatg acgctgcgcc tttcagactg tgacctgaga gagatttgta gcgaggtcgt
     1081 ctcggaacaa caatcccttt caggacgtaa aatcgagctt gagattcccc catcacctgt
     1141 agtcctgcaa gccgatagcg aacgcctcag ccaggtagtc acaaatctac tcaccaacgc
     1201 cctcaaatat tcgccagaag aaagccccgt acaggtacag gtcagccagt caaaaaataa
     1261 agccatcatc cgcgtacaca acgacgggca gcccatcccc aaagaacagt tagccagcat
     1321 tttcgagcca ttttaccgcg ctcccgccgc ccaaaaatca tcaaagaaag gctggggcct
     1381 cggcctggcc atcagccagc aaatcgtcga tcgccacaac ggtcgcatct gggtagagtc
     1441 tagcgagggc aaaggcacca cgttcagtgt agagctgccc atgaaacagc gcatcgaaca
     1501 agcgcaagaa atgggacgca gctacatccc ccaggatcgg tcgctggctg gtgaactcct
     1561 tgctgagcga aggcgagaag gcgagtaaga gcaggctagt gtcgcaaatc tttgagaatc
     1621 gctggcatag gatagtgtac tgtcgggaga tgttcccagc gtatcgtccg ctcttcgggg
     1681 attggtgcgt cgggattggg ggtttcctcc cactgcagcc cggcggattc ggcacgtaat
     1741 tgaagttgtt ctgcctcttg ccctctcccc agttgttcca gcaggatgct gagattgcgg
     1801 tagaacagca cttcgccagg ttcgagttcg atagcctgtt catacgcccg aatagcttgc
     1861 tcatcgtgct tcatattcgc aagcaggaca cccagattgt tatagagacc ggcatctcgc
     1921 ggataacgtt gcagcgcttg agcataggtg acgagggctt ctggttcgcg ttgtagttca
     1981 ctgagaacct ctgcttttca gtcatagagc gcaacttcct caggaacgag cgccatcgct
     2041 tgctcaaagg tggtcagggc ctccgcatag cgatgcagct tcttcaacac ttcccctttg
     2101 tacaaataga aatgtgggaa ggtcggatcc aggccaagga cacgttgata gacagccaac
     2161 gcggattcgt acaggcccag cttcttgcat aactcagcca ggtattcata ggcagaggcg
     2221 tcctgtggat tgcagatgat ggcttgctca taggcagaga gggcatccgc atactgttcc
     2281 tcatgttgga gacgatggcc tctattgatc cattgagccc aggtctccgg tgcaggttca
     2341 ttgaagattg tcgatgccga catgaaactt ccccccttat gtatctctcc tccatcatat
     2401 accaagcagt caatatttca gcatggatca tgcctgacat tatgttataa taccctctga
     2461 acactcgttc ttacctataa agagtatcga ctggttcgga gcgtacacta tcatgcccat
     2521 cctacaactc gcccccgatg tcgccgccaa gatcgccgcc ggcgaagtcg tcgagcggcc
     2581 tgcgtccgtc gtcaaagaac tgatcgagaa cagcatcgat gccggagcca cacacattcg
     2641 cgtcgagctg gtcggcggcg gtctgtcgct catccacgtc accgataacg gcggcggcat
     2701 cccggcgggc gaactgcccc tggccttatc gcgccacgcc accagcaagg tcgccagcat
     2761 cgacgacctg gagcatatcc gctcgctcgg ctttcgcggc gaggcgctcg ccagcattgc
     2821 cgctgtcgcc gatgtcacgc tcaccagccg ccatcgcgag gaacagcagg gcgcgcaggt
     2881 ctccgccagc aacggacaca tcagcgaagt catggcggcg gcctctccgg ttggcacaac
     2941 catgaccgtg cgcaatctct tcagcgccgt gccagcccgc ctgaaattcc tcaagagccg
     3001 cacgaccgag gttagccact gccatcacct gctcgaacaa tacgcgctgg cctatcccga
     3061 aattcgcttc agcgtctcca gcgaagggcg gcagatcttc gccacccccg gcgacggcaa
     3121 gatcagcagc gtcctggtcg ctatctacgg cttagaggtc gctgagcaga tgatccccgt
     3181 cagcagcctg gatgggcgcg acgaagaccc attgcggcca gtcgccaccg gctacatcag
     3241 caagcccacc tgctacaaaa gcacccgaca gtacatgtcc ttcttcgtca accgccgctg
     3301 ggtcatgagc cgtatgctca gcgcctccat cgaggaagcc tataaaacgc tgctgctcac
     3361 cggcaggcac cccatcgccg ttatcaacat catcatcgac ccatcccaga tcgatgtcaa
     3421 cgtccatccc gccaaaaccg agatccgctt cctgcgtgag cggcgcgtct actccgccat
     3481 cgtgcgagca gtgcggaacg ccatcctcga aggcgctgaa atgccgcaat ggggcagcgc
     3541 atcatccacc gccccatcct cctcgcacga tgacacgtct ctcatgcgcg atttgcccgg
     3601 cgatctccct gaagacatgc ctgccgaatc gaccaaccct accggtcatg gcaacgaggc
     3661 ttttccgcga gacaacccct ggttgaccgt tactgaagaa gaaaccggaa caaaaccgcc
     3721 cctgctctcg cgcctctggc aaagccacgt gcgcggccag caggcgctgg gcaccgagat
     3781 ccttcgcttc gctcaggatg acaggactgg ttttgatgat gacatgacag gttttgatcg
     3841 tgaacattca ttatctggct ctactgatac atcggataca tcggatacaa acccggcaca
     3901 tccaaacagc cctccgcgtc tatccaatac gagcgacacg agcgatacat tcgctgcaac
     3961 aggctccatc caggcatcaa ccagcatgag tctactgccc atcctgcgcg tcatcggcca
     4021 gctttcgcag agctacatcg tcacggaggg gccggacggc atgtacctgg tcgaccagca
     4081 cgccgcgcac gagcgcatcc tgctggagcg catggtggcg gcgttgcagg cgcgcgccac
     4141 actctcacaa ctgctgctga ctcctctgcg cctggaactt gcgccggtcg aactggaagc
     4201 ccttgaagaa catcttgagc aactgcgcgg catcggcttc accctggaaa tcctggaaga
     4261 tggcgcgctt gaggcgcgcg ccgtccccca ggcgctgctc aaacagatca acgcgcgctc
     4321 gctgcgagac ctgctggttg aactgaccgc gccgggcttc gatggcaaca acgagcgcaa
     4381 tggaggcagc caggcctggc aagaacacgc cctggccaac gtcgcctgca aagccgccat
     4441 caaagccaac tacttcctga cggtcagcga gatgcgcgag ttgatcgagc aactgggaca
     4501 gaccggcgcg ccctttagct gctgtcatgg ccgccccacg atggtgcatt tcggcctgtc
     4561 cgcccttgag cgcgaatttg ggagacgcta agtgtttgcc gaagtgctga cctctgccgc
     4621 caggtggcag accgaacaac ccctgctgac ctacatcgtg ccggtcacac tggagcagga
     4681 agcgcgccca ggacaactgg tcgccgttcc ctatggcgag cgactggtcg aaggcatcat
     4741 ctggcgactc acaggacacc ttgaagacac cgaccttcct caacccgcag ataacccgcg
     4801 tgaaaggaca tcgcaggccc tcactctcga ccagttacgc cccatttccg ccatcctcga
     4861 ccccgaaccg gcgctgctgc cccaccagcg cgcgctggcc gagtggatgg caacctacta
     4921 cgtcacaccc ctcgcacagg tcgcgcaaag tatgctgccg cccgggctgg cgcagcgctc
     4981 aaaggtcgtg ctacgcctca tctcagacga cccgcaaccg gaagtcgaac agccggggca
     5041 accgtccatc acatccacac gcgcactcat cggcctgctg ctggccgatg gcgacctgga
     5101 tatcgcgcgc ctcaaagaga tgctggggcc gaaacgcgcc ggagaggtca tcaaagaagc
     5161 cctggcaagc gggctgatcg cgcgcgaggc gcagctagac cagccgcgcg cccgcgcccg
     5221 ccgcaagcgt ctcgtgcggc tgcgcgcgcg cggtgaggaa cttgaggcgt ggaaggagcg
     5281 cgccaggacc agaatccagg aaagcctgcc cgaccccacc actatgccgc tcgcgcctga
     5341 taatgtgcgg cggcgtccca caaaacacgc gcccgatccc tgggctatcc ccggcgcatc
     5401 gtcggtcttc acgctcaccc gcgagaaccg tgttggcctg ctcgcacagc gacaactggc
     5461 cgccatcgac ctgctcgaac acgatcgcgc cgatgccagc gccgaagcgc actggacgcc
     5521 caccatgctc tgcaaagcca gcagcctgca cgcccgccag cttacagccc tggcgcaaga
     5581 aggcatcgtc accatcgaag agctggaggt gcagcgcgac ccactctccg ggcgcaacat
     5641 cccacccagc gcgcccctgc ctttgacggc tgaacaacgc gacgcgctat cggcgatcgt
     5701 gggcgatacc cccatcctcc tgcacggcgt gacgggcagc ggcaaaaccg aggtctattt
     5761 gcaggcgctg gcggccatca tcgcgcgggg ccagcgcggt atcgtgctgg tgccggagat
     5821 cgccctgacc gcccaggcca tctaccgttt cgccgggcga tttcctgggc gcgtcgcggt
     5881 catccacagc gcgctgagcg atggcgagcg atacgacgaa tggcggcgca tccgggccgg
     5941 caaggtggat gtcgtggtcg ggtcgcgctc cgccctcttc tcgcccgtgc cagacctggg
     6001 actgcttatt ctcgacgagg aacacgaacc ggcctataaa cagagcgagc gtaagccgac
     6061 ctatctcgcg cgcgacaccg ccatcgcgct ggcaaagctg ctgcatatcc ccgtcgtgct
//
LOCUS       DASZAK010000293         3841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_540008, whole genome shotgun sequence.
ACCESSION   DASZAK010000293 DASZAK010000000
VERSION     DASZAK010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540008"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            338..610
                     /locus_tag="VGT44_05155"
     CDS             338..610
                     /locus_tag="VGT44_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEV2580221.1"
                     /translation="MATVRLAPVLRSSVGGAKQVTAQGGTLNEVLYDLYQRFPALKEQ
                     IQPEETLSRYINVYINDQDVRYLQGIESAVGPNDTVILLPAMAGGR"
     gene            627..1562
                     /locus_tag="VGT44_05160"
     CDS             627..1562
                     /locus_tag="VGT44_05160"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEV2580222.1"
                     /translation="MIYGSILETIGNTPLVELKSFSPRPGVQIFAKLEGANPSGSIKD
                     RIAMKMVDEAEADGRLKPGAILLEPTSGNTGIALAMIARVKGYEFVAVMPDNVTQERR
                     QMLDLYGAQMVFSDGALGSNGAVRLAQELAGADDRYVMLYQYGNSANPRAHYEGTAPE
                     IIRDLPDVDVFVAGLGTGGTLTGNARRLKEYNPAIKIVAAEPLQGDGVQGLRSLEDGF
                     VPPVLDQSLLDAKILVSGLDAVRRTRQLKDQEGIFAGPSCGAALHAALRVAATMEHGK
                     IVVILADGGWKYLSEDLWTRNLDTLEDELEETLLW"
     gene            1556..1981
                     /locus_tag="VGT44_05165"
     CDS             1556..1981
                     /locus_tag="VGT44_05165"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HEV2580223.1"
                     /translation="MVANGPFVVRIPEAIYEAMTAHVVAGYPNEACGALGSKDGQVVK
                     HDPTANAAEQPDDFSIIAPEVLLDIFDDIDTYDGEMIYYHSHPISEAYPSRRDKDWAK
                     SNRLYYIIYSHRFYPEPPYARVFLVHEDDSVTEGSIELV"
     gene            complement(1978..3195)
                     /locus_tag="VGT44_05170"
     CDS             complement(1978..3195)
                     /locus_tag="VGT44_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2580224.1"
                     /translation="MRCRYCNAELAAGAIFCQRCGKPVENDEFSSYGYDTGEQSFSVP
                     PIPPAASDSPNATPPQPTPSTPALQPSAADQQQPAQPVQPTQPGQPSQQEPPAQQMYP
                     GQPIGPTMQPYVPQQAYYNPQYYQGAGPFPQAFQPTPGMFPPVVPPKRTGLSPTVVIL
                     LVVLALLVGGGGVFAFFSLSHRSPPSSQTPGTSTPTRTNATGTTGDPLQLYTRATSGS
                     PTLVDPLDATNPNGWQAISSGGACTFAGKTLQLSAKAAGSGKSAIGVCLALATDFSDF
                     AYQVNTTISQGDIAGLTFRTDPFAGVVYQFGIDDTGGYILYTLVTNSAGKTALKILTE
                     GSNPAIKAGANQSNVLTVIAQGSSINLYANQQYLASVTDSTASGGEIGLFGENTNGGA
                     VNITYSSIKVWQL"
     gene            3515..>3841
                     /locus_tag="VGT44_05175"
     CDS             3515..>3841
                     /locus_tag="VGT44_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2580225.1"
                     /translation="MVLDTGTDLASALLDVMPRLLRRLGAELPLDGDALNDPAWREVV
                     ELRATPGQLSLMCVLVEHKCCTMQEIAEHVGVAPSTATAMVKRLVAQGYIERSRDEAD
                     WRAVWVR"
BASE COUNT          813 a         1063 c         1145 g          820 t
ORIGIN      
        1 acctacgacg ccctggccgg tgagttcgcc gaactgcgcc tcttccgcga cccgcactgc
       61 ccggcctgcg gtgaagacgc ccatccgcag gacctgccga cctacgctga gttctgcgcc
      121 atgcctgcaa gatcgtaaga gatatatcaa tccgtagagc tgattatgaa atttaacgaa
      181 cgagatcggg aatgtcatcc tgagcgcagc gaaggatctg gcagcctatc caccgagatc
      241 cttcgctgcg ctgaggatga cagcaacgac tttggtcatg aaacttcatt atcggccctg
      301 acgcaagtgg aaattatata aggagaacct atccatcatg gcaaccgttc gacttgcgcc
      361 tgtcttgcgc tcctccgttg gcggcgcgaa acaggtcaca gcccaggggg gcaccttgaa
      421 tgaggtgctg tacgatctct accagcgttt cccggccctc aaggagcaga ttcagcccga
      481 agagaccctg agccgctata ttaatgtcta tatcaacgac caggacgtgc gctacttgca
      541 ggggatcgaa tcggcagtcg gcccgaacga caccgtgatt ctcctgcctg cgatggcggg
      601 cggacgctaa cccgccgggg gattccatga tttacggcag catacttgag accatcggca
      661 atacaccctt agtcgagctg aaaagcttca gcccgcgtcc cggtgtgcaa attttcgcca
      721 agctcgaagg cgctaaccct tccggcagca tcaaggatcg catcgcgatg aagatggtcg
      781 atgaagcgga ggccgacggc aggctgaaac ccggcgcgat tctgctggag ccgaccagcg
      841 gcaataccgg catcgcgctc gccatgatcg cgcgcgtcaa gggctacgaa ttcgtggccg
      901 tcatgcccga taacgtcacc caggaacggc ggcagatgct ggatctgtac ggcgcgcaga
      961 tggttttttc ggatggggcg ctgggcagca atggcgctgt gcggctggcg caagaactgg
     1021 ccggggctga tgatcgctat gtgatgctgt accagtatgg caacagcgcc aaccctcgcg
     1081 cgcactacga aggcaccgcg ccggagatca tccgcgacct gcccgatgtg gatgtgttcg
     1141 tagccggact cggcacgggt ggcacgctga ccggcaacgc gcggcgtttg aaggaataca
     1201 atccggccat caagatcgtc gctgccgaac cgctgcaggg tgatggcgtc cagggcctgc
     1261 gcagcctgga ggatggcttc gtcccgcctg tgctggatca atcgctgctc gatgcaaaaa
     1321 tcctcgtctc tggcctggat gccgtccgcc gcacgcgaca gttgaaggac caggagggca
     1381 tctttgccgg tccatcctgc ggcgcggcgc tgcacgcggc actgcgcgtg gcggccacga
     1441 tggaacatgg caagattgtc gtcattctgg cagatggcgg ctggaaatac ctcagcgaag
     1501 acctgtggac gcgcaacctg gacactctgg aagacgagtt agaggaaacc ttgttatggt
     1561 agcgaatggg ccgtttgtcg tgcgtattcc tgaagcaatt tatgaagcca tgaccgcgca
     1621 tgtcgtcgca ggttatccca atgaagcctg cggcgcgctc ggcagcaagg atggccaggt
     1681 cgtcaagcat gatccgaccg ccaatgccgc tgaacagccc gacgatttct ccattatcgc
     1741 ccccgaagtc ctgctcgaca tcttcgacga catcgacacc tacgatggtg agatgatcta
     1801 ctatcactcc caccctatca gcgaagccta cccctctcgc cgcgacaagg attgggccaa
     1861 aagcaaccgc ctctactaca tcatctactc ccaccgcttc taccctgaac caccctacgc
     1921 gcgggtgttt ctggtgcatg aagatgattc ggtgacggag gggagcatcg aactcgttta
     1981 gagttgccat actttgatcg aactgtaggt gatgttgacc gcgcctccat tcgtgttttc
     2041 tccgaagagg ccgatttcgc cgccgctggc ggtggaatcg gtgacgctgg ccagatactg
     2101 ctggttggcg tacaagttaa tgctgctgcc ctgcgcaatg actgtcaaaa catttgattg
     2161 gtttgctcct gctttgatcg cggggtttga tccctcggtc aagattttga gcgccgtttt
     2221 tcccgcagag ttcgttacca gtgtgtagag gatataccct cccgtatcat caatgccgaa
     2281 ctgatagact accccggcaa acgggtcggt acggaatgtt agccccgcaa tgtcaccctg
     2341 cgagatggtc gtattcacct ggtacgcaaa atcgctgaag tcagtagcca gcgccaggca
     2401 gacgccgatt gccgattttc cagacccggc agccttcgcg ctgagctgca gcgttttgcc
     2461 tgcaaatgtg caggcgccgc cactgctgat ggcttgccac ccgttcgggt ttgtggcgtc
     2521 cagcggatca acaagcgttg gcgagccact cgtcgcccgc gtgtagagct gtagcgggtc
     2581 gccggtggtg ccggttgcat tggttcgcgt cggagtagag gtccctggtg tttgtgatga
     2641 cggcggcgac ctgtgcgaaa gcgaaaagaa agcgaacact cctccgccgc caaccagcag
     2701 cgccagtacg actaagagga tcacaacggt aggtgacagg ccggttcttt tcggtggaac
     2761 aacgggagga aacatgccag gtgttggctg gaatgcctgg ggaaatggtc cagcaccctg
     2821 gtaatattgt gggttatagt atgcctgctg cggcacatag ggctgcatag ttggcccgat
     2881 tggctggcct ggatacattt gttgcgcagg cggctcttgc tgggaaggtt ggccgggttg
     2941 cgtgggctga acgggctgcg cgggttgctg ctggtctgcg gcgctcggct gtagagctgg
     3001 tgttgaaggt gttggttgag gaggagtggc attgggtgag tcggatgcag caggcgggat
     3061 gggcggaaca gaaaatgatt gctccccggt atcgtatccg taagaagaaa actcgtcatt
     3121 ttcaaccggt ttgccacaac gctggcaaaa tatcgccccg gctgccagct ctgcgttgca
     3181 gtatcgacat cgcatagttg tatgctcctt caagaaaaga tgaaaaagga taagaacacc
     3241 gtaacaatta tgcctgtgcg ccaaataata tagtgcagta tatcgcatac tatagtcatt
     3301 taccatagac aggcatttgg aaaacgtgcc tgccatcctg ccgtggctgt catcatgtcg
     3361 tgcctgtcat cctgagcgca gcgaaggatc tctctcgcct gccgcagatc cttcgctgcg
     3421 ctcaggatga cagcaggata agatagggct tgacccgctt atttatttcg tctatcatat
     3481 aattagacaa tcaaagtatt taaggggaaa atcgatggta cttgatacag gaacggacct
     3541 ggcatcagcg ctcctcgatg tcatgccgag actgctgcgc cgcctgggag cagagctgcc
     3601 tctggatggc gacgcgctca atgatcctgc ctggcgcgag gtggttgaac tgcgcgccac
     3661 gccgggtcaa ctgagcttga tgtgcgtcct ggtcgagcat aaatgctgta ccatgcagga
     3721 aatcgctgaa catgtagggg ttgcgccatc gactgcaacc gcgatggtca agcggctcgt
     3781 cgcccagggt tatatcgagc gcagccgcga tgaagcggat tggcgcgctg tttgggtcag
     3841 g
//
LOCUS       DASZAK010000294         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_113199, whole genome shotgun sequence.
ACCESSION   DASZAK010000294 DASZAK010000000
VERSION     DASZAK010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113199"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..2419
                     /locus_tag="VGT44_05180"
     CDS             134..2419
                     /locus_tag="VGT44_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2580226.1"
                     /translation="MQTIGHGGMAAVYQARDTKRGTMCAIKEMSLSTVAPGEQPLAVQ
                     NFLAEARILSRLNHANLPAFTDFFTEDERRYLVMEFIDGSTLEDLLEQQGRPFSERRV
                     LSWARQLCDVLEYLHSQQPPVIFRDMKPGNIMLSRNGRIKLIDFGIARLFRPSGGAQD
                     TQLLGTPGFSPPEQYGGAQTDERSDIYSLAMTLFQLMTLSVSEKGFGLTDVHANFPYI
                     SPPVARALEKATLLRQEDRYESVVVFRRALLGIDTFRFENGGEATTPQELADLCARYP
                     EEAGDYLYSGEIESWLREIGANDVARATGRIRSTVGDPALGVNKLLQAIMGPNAQTRT
                     ASASSTVTQASVPPSRGSLISRVRDPNTVVVRPRTIDFGQTYPGLTAPILLTITGAKG
                     ALVSGTIRPVESWIIIDTTTFDGLSTPVRVRADSTRLTGSSHYTGKIIIQPDDSEREI
                     IVTVELEVLEHTTVTMPRVSGQTGSSTLQSGSQVMKSGQQGASRVRSAASSPGAQQAT
                     AARAGVAANASRANQSAPPADQPFTNARYDEYKTKYGAPGSGGWDMLKTTARQRAFMR
                     YAITFAASLMSGALVYLLLARIPAIAHASILPPSGWFVALLAGFLVFCTLGAMLMNLG
                     TIWANRAVLTRGGSDLATALLFLGLGEATWQNLAFPGSQLQVLVMLFLTAAGATFGIA
                     PRVSDWIIRSSTWLMTRAVWLAFSIAAIVGAILGYGLTSGPSYGCFAPFGALGGAFIG
                     VALVLRIRRVIRKKNASTPTP"
     gene            2416..>2595
                     /locus_tag="VGT44_05185"
     CDS             2416..>2595
                     /locus_tag="VGT44_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl transferase family protein"
                     /protein_id="HEV2580227.1"
                     /translation="MNEQHQQLPPLVVVLGPTASGKSALGIELARRFVGEIVSADSRQ
                     VYRGLDLGTAKVTPVE"
BASE COUNT          494 a          897 c          719 g          485 t
ORIGIN      
        1 atactgtgac acaatgcgtc tttgagagag atgggagacc aggatcatta gctcgcagaa
       61 cggcaatggc aaattcaata cgcagacggg gcagctcgac ccgcataccc tgctgcacaa
      121 acgttatatc gttgtgcaaa caatcgggca tggcggcatg gcggcggtct accaggccag
      181 ggacacgaag cgcggcacta tgtgcgccat caaagaaatg agcctctcga cggtcgcgcc
      241 cggcgagcag cccctggctg tgcaaaattt cctggcagag gcgcgtatcc tctcgcgcct
      301 caatcatgcc aatctccccg cctttaccga tttcttcacc gaggacgagc gccgttacct
      361 cgtcatggaa tttatcgatg gtagcacgct tgaagacctg ctcgagcagc agggccgccc
      421 cttttctgag cggcgcgttt tgagttgggc caggcagttg tgcgatgtat tggagtattt
      481 gcatagccag cagccgcccg tcatctttcg tgacatgaaa cccggcaaca tcatgctctc
      541 gcgcaacggg cgcatcaagc tgatcgattt cggcatcgcg cgcctgttcc gtcccagcgg
      601 cggcgcgcag gatacgcaac tgctcggcac ccccggcttc tctccgccgg agcaatatgg
      661 cggcgcgcaa accgacgagc gttccgacat ctactcgttg gctatgacgc tctttcaact
      721 gatgacgctc tccgtctccg aaaaaggctt cgggctgacc gacgtacacg ccaacttccc
      781 ttatatctcg cccccggtag cgcgcgccct ggaaaaagcc accctgttgc gacaggagga
      841 tcgttacgaa agcgtggtcg tcttccgccg cgccttgctg ggtatcgata ccttccgttt
      901 tgaaaacggc ggcgaagcca ccactccaca ggaactcgcc gatctctgcg cccgctatcc
      961 agaggaagcc ggcgattatc tctactccgg tgaaatcgaa tcctggctgc gagaaatcgg
     1021 cgcgaacgat gtcgcccgcg ccaccgggcg catccgctcc acggtcggcg atcccgccct
     1081 gggcgtcaac aagttgctgc aagccatcat gggaccgaac gcgcagacac gcaccgccag
     1141 cgcttccagc acagtcacgc aggcgagcgt ccccccatcg cgcggcagcc tgatctcgcg
     1201 cgtgcgtgat cccaacaccg tcgtcgtgcg tcccagaacc atcgatttcg gccagaccta
     1261 ccccggcctg accgcgccca tcctgctcac catcaccggc gcaaaaggcg cgctggtcag
     1321 cggcaccatt cgcccggtag agtcctggat catcatcgac actaccacct tcgatggcct
     1381 cagcactcct gtgcgtgtac gcgccgatag cacccgcctg accggatcga gccactatac
     1441 cggcaaaatc attatccagc ccgacgacag cgagcgcgaa atcatcgtca cagttgaact
     1501 ggaggtactc gaacacacca ccgtgaccat gccgcgtgtc tccgggcaga ccggctcatc
     1561 gacgctccag tccgggtcgc aggtgatgaa gtcggggcaa caaggcgcat cccgcgtgag
     1621 aagcgccgcc tcatcgccag gcgcgcagca ggccaccgcc gcgcgagcag gtgtggcagc
     1681 gaacgcaagc agggctaacc agtccgcccc gccagcggat caacccttca ccaacgcgcg
     1741 ctatgacgaa tacaagacca aatacggtgc tcccggcagt ggtggctggg acatgctcaa
     1801 gaccacagcc cgccagcgcg ccttcatgcg ctacgctatc accttcgccg cgtctctcat
     1861 gtccggtgcg ctcgtctacc tgctccttgc gcgcatcccg gctatagccc acgcctcgat
     1921 tctaccgcca agtggctggt ttgtggccct gttggccggt ttcctggtat tctgcaccct
     1981 gggcgctatg ctgatgaacc tgggcaccat ctgggccaat cgagctgtac tcacacgcgg
     2041 tggctccgac ctggcgactg cgctgctctt cctcggcctt ggcgaagcca cctggcaaaa
     2101 tctcgccttc cctggctcgc agttgcaggt tctggtcatg ctgttcctga cagcagcagg
     2161 tgcaacgttc ggaatcgccc cgcgtgtcag cgattggatc atcagaagca gcacatggct
     2221 catgacgcgt gccgtctggc tggccttctc tatcgccgcc atcgtcggcg cgatcctcgg
     2281 ttatggactg acgagcggcc cctcctacgg ctgtttcgcc cccttcggcg cgctcggcgg
     2341 ggccttcatc ggcgtggcgc tcgtgctgcg catccgccgc gtgatacgca agaaaaacgc
     2401 atcgactccc acgccatgaa cgaacaacat cagcagcttc cgcccctcgt cgtcgtcctc
     2461 ggcccgaccg cctccggcaa gagcgccctg ggcatcgagc tggcgcggcg tttcgtcggc
     2521 gaaatcgtct ctgccgactc ccgccaggtc taccgcggtc tcgacctcgg caccgctaaa
     2581 gtcacgccag tggaa
//
LOCUS       DASZAK010000295         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_256473, whole genome shotgun sequence.
ACCESSION   DASZAK010000295 DASZAK010000000
VERSION     DASZAK010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256473"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VGT44_05190"
     CDS             <1..312
                     /locus_tag="VGT44_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580228.1"
                     /translation="ALAGSDHTVEVWKLAERRKVLTYRGHRQAVLSVAWSPDGRHIAS
                     GGKDHTVHIWDVSKNAQTQQSNFTYCGHKESVRSVAWSPDGQFIASGSDDCSVHVWQA
                     I"
     gene            788..1297
                     /locus_tag="VGT44_05195"
     CDS             788..1297
                     /locus_tag="VGT44_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580229.1"
                     /translation="MMPGTIQTMAWAYKQICAGEDPWTALGNFTNAWYDYAHDIRATL
                     VSEPLAEPERTTEHTSRWGAFCAASVEFLCDRYGIPCPEWVYNSHYILATPWWHTSHP
                     HSLSIRERLVHKTPEPFARRNIFCGNRLYQNKYEMSVWVQEARAKGVTDPGEVWRYAR
                     QKEISIHGA"
     gene            complement(1329..2156)
                     /locus_tag="VGT44_05200"
     CDS             complement(1329..2156)
                     /locus_tag="VGT44_05200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2580230.1"
                     /translation="MADMQESEILKANIAALYSRAAPLYGQVGPGRFAYVGRGLVERL
                     GIGEGAQVLDVAAGRGANLFPAAEKVGPRGQVTGIDLSEGMVRETADEIARRDVRNAA
                     VMQMDAEQLTFDAASFDYILCGFAIFFFPHLGRALAGFHRVLRPGGKLGISVANDRDP
                     LSQWYGKHVTEYATHYHIPMRVGRGSLDFDEIPAHLVRAGFTKTELMREQADLPYASA
                     QEWWDEKWTHGTRYALEHMTPEVLAQFKDEVFVRLAEEQQAGGIREVLQIQYHIATK"
     gene            complement(2169..>3995)
                     /locus_tag="VGT44_05205"
     CDS             complement(2169..>3995)
                     /locus_tag="VGT44_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEV2580231.1"
                     /translation="AEVRAAMDGAKSGAAHSITLDGVSVEVDAPFASPQDWRDQWIYF
                     LMLDRFNNPAAPPRQLPYDSTYGQFQGGTYNGVRAQLPYLKGLGVGAIWITPVLKNCQ
                     YSQGSYHGYGIQDFLNVDPRFASSPDVAERELRALVDEAHRLGIYIIFDIVLNHTGDV
                     FAYDCQAGDATCQGSDGSEATYSAAPYGIAWRDEHGNARQDWPVGENIVNPSPDAVVW
                     PSELRRNAFFRRQGLPQNGGDPTIGDFASLKQMLTAYNDLQVVLVRAYQYLIAKYDID
                     GFRVDTLKYIDRGLARLFGNAMREFGLSIGKKNFFTFGEVYDNEQKITAFIGRDTLDT
                     ETGDPIGIDAALDYPLFFRLPGACKGMLAPMDVASMYEQRKQIDRDILSSHGDASNFF
                     VTFLDNHDQAQRFYYSPTSAPHTYDDQLTLALACLYCLQGIPCLYYGTEQGLHGSGSV
                     PEAVREALWGKPNAFDTSNPFYQAIQRIAQARAARPALRYGRQYFRPVSGDATAFGVS
                     TFAPGVLAFARILNDEEALVVANTTTQAETLAVIVDETLSAPGDVFKVIYSNKPAYSD
                     PGRVTQSGPNTTVNQVDGTTTTGAVHYITVTLQPMEAQILGQ"
BASE COUNT          867 a         1163 c         1145 g          820 t
ORIGIN      
        1 gcactggcag gtagcgatca caccgttgaa gtctggaagt tagcagaacg ccgcaaggtg
       61 ttgacctatc gcggccatcg acaggccgtc ctgagcgtgg cgtggtcgcc cgatggcagg
      121 cacattgctt ccggtgggaa agatcataca gtgcatatct gggatgtctc gaaaaatgcg
      181 caaactcagc agtcaaattt tacctattgc ggccacaaag agagcgtccg gagtgtcgcc
      241 tggtcgcctg atggacaatt tatcgcttcc gggagtgacg actgcagcgt acatgtgtgg
      301 caggcgattt gaaatcgtac acacattctt cgccgattcg tactcacacc cagatctgaa
      361 ttggattctt ccttaataga agcaggggct gtggctatcg tgggtcgtcc tggctacttt
      421 atccagagat aacaaattct tggccgcatg tgttatactc aggtggatgg aagcgaagga
      481 gattgtgctg atgtacgacc agatccatat tccccacggc aggtgatgga gacagtacgg
      541 accgcttcat atctatgtcc cgccaaaaga atacattctt gcgctcaaga tattggcagg
      601 acgagagaaa gatattgaag attgtaaact actgctccag atgacaaaag taaaaacacg
      661 ccaacaagcc cagcgattgc tcgatcgcta tattctcact aatgcgcaac gggataaaac
      721 tgaacagatt gaaagctcac ttgacgagct tttcggcaaa gcataggtac gtgaaggaga
      781 gcgagcgatg atgcctggaa ccatccagac aatggcatgg gcctacaagc aaatctgcgc
      841 gggagaagat ccctggacgg cgctcggcaa cttcaccaac gcctggtatg actatgcgca
      901 tgacatccgg gctactttgg tcagtgagcc attagcagaa ccggagcgta ctacagaaca
      961 caccagtcgg tggggagctt tttgcgcagc ctccgttgag tttctctgtg atcgctacgg
     1021 tataccctgt cccgaatggg tctacaattc gcattatata ctagcgactc cctggtggca
     1081 caccagtcat ccacactctt tgtctattcg agagcgcctg gtgcataaaa cgccggaacc
     1141 gttcgcacga cgcaacatct tttgcgggaa ccggctctat cagaacaaat acgaaatgag
     1201 tgtctgggtc caggaagcac gggcgaaagg tgtcaccgac ccaggcgaag tctggcgcta
     1261 tgctcgtcag aaagaaatca gtatccatgg agcctaaata gaactgaata tcaaaaacga
     1321 agcctctttt acttcgtcgc gatatgatac tgaatctgca atacttcgcg aatgccaccc
     1381 gcctgctgct cctccgcgag ccgcacaaac acctcgtcct tgaattgcgc cagcacctcc
     1441 ggtgtcatat gctcaagtgc gtagcgcgtg ccgtgcgtcc acttctcgtc ccaccactcc
     1501 tgcgcgctgg cataaggcag gtccgcttgc tcgcgcatca actcggtctt cgtaaaaccg
     1561 gcacgcacca ggtgcgcggg gatctcatca aagtccaggc tgcctctgcc aacacgcata
     1621 ggaatgtgat agtgcgtggc atactcagta acgtgcttgc cataccattg tgagagcgga
     1681 tcgcggtcgt tcgccacaga aatgcccagc ttgccgcctg ggcgcaacac gcggtgaaag
     1741 cccgccagcg cccgccccag gtgaggaaag aagaagatgg cgaagccgca taggatatag
     1801 tcgaagctgg cagcatcgaa agtgagctgc tcagcatcca tctgcatgac ggcggcattc
     1861 cgcacatcgc ggcgcgcgat ctcgtcggcg gtttcgcgca ccatgccctc cgacaggtcg
     1921 atgccggtca cttggccccg cgggccaact ttctccgccg ccggaaagag gttcgcgccc
     1981 ctaccagccg ctacatcgag cacctgcgcg ccctcgccga tgcccagtcg ttcgaccagc
     2041 ccgcgcccga cataggcaaa cctgcccggg ccgacctggc catacaacgg tgccgcccgg
     2101 ctataaagcg cggcaatgtt ggccttcaga atttcgcttt cctgcatgtc ggccatcgct
     2161 tatctctcct actgccccaa tatttgcgcc tccatcggct gcaaggtcac cgtgatgtag
     2221 tgtaccgctc cggtggtggt cgtgccatca acttgattga cggtcgtatt cggaccgctt
     2281 tgcgtcacgc gcccaggatc gctataggcg ggtttgttgc tgtaaatgac cttgaataca
     2341 tcccctggcg cggagagtgt ttcatcgacg atcacggcca gtgtttctgc ctgcgttgtc
     2401 gtattggcaa ccaccagcgc ttcttcatcg ttgaggatgc gcgcgaaagc cagcacgccg
     2461 ggcgcgaagg tggagacgcc gaaggccgtg gcatcgccgg agacaggacg gaagtactgg
     2521 cggccatagc gcagtgcggg ccgggcagcg cgcgcctggg cgatgcgctg gatggcctgg
     2581 tagaaggggt tggaggtatc aaaagcgttc ggtttgcccc atagcgcctc gcgcaccgct
     2641 tcggggacgc tgccgctgcc gtgcagcccc tgctcggtgc cgtaatacag gcaggggatg
     2701 ccttgcaagc agtagaggca ggccagggcg agcgtcaact ggtcgtcgta ggtgtgcggc
     2761 gcgctggtcg ggctgtagta gaagcgctgc gcctggtcgt gattatccag aaaggtgacg
     2821 aaaaagttgc tggcatcgcc gtgcgaactc aggatatcgc gatcgatctg cttgcgctgc
     2881 tcgtacatgc tggctacatc catcggcgcg agcatcccct tgcaggcgcc gggcaaccgg
     2941 aagaagagcg ggtaatccag cgcggcatcg atgccgatgg gatcgccggt ctctgtatcc
     3001 agggtatcgc gtccgatgaa ggccgtgatt ttctgctcgt tgtcgtagac ttcgccgaag
     3061 gtgaaaaagt tcttcttgcc gatgctcagg ccaaattcgc gcatggcgtt gccgaagagc
     3121 cgcgccaggc cccggtcgat gtacttgagc gtatcgacgc gaaaaccatc gatatcgtat
     3181 ttggcgatca ggtactggta ggcgcgcacc agcactacct gtagatcgtt ataggcggtg
     3241 agcatctgct tgagcgaggc gaaatcgccg atggtaggat cgccgccgtt ctggggcaat
     3301 ccctgccgcc ggaaaaacgc attgcggcgc agttcgctcg gccagaccac cgcgtcgggg
     3361 gatggattga cgatgttttc cccgacaggc cagtcctgcc gcgcgttgcc gtgttcatcg
     3421 cgccacgcta tgccgtaggg tgccgcgctg taggtcgcct ccgaaccgtc gctgccctgg
     3481 cacgtcgcgt cgcccgcctg gcaatcgtag gcgaagacat cgccggtatg gttcagcacg
     3541 atgtcgaaaa tgatgtagat gcccagccgg tgcgcctcgt ccaccagcgc gcgcagttcg
     3601 cgctcggcca cgtccgggct ggaggcaaaa cgcgggtcaa cattgaggaa atcctgaata
     3661 ccgtagccat ggtagctgcc ctggctatac tggcaattct tcagaaccgg tgtaatccag
     3721 attgcgccaa cacccagacc tttcaggtaa ggcaactgag ctcgcactcc attatacgta
     3781 ccgccctgga actgcccata agtactatca tagggcaact ggcgcggcgg tgcggcggga
     3841 ttattgaaac gatccagcat caaaaaatag atccactggt cgcgccagtc ctgaggcgac
     3901 gcgaacggcg cgtccacctc cacgctcacc ccatccagcg taatgctatg cgcggccccg
     3961 ctcttcgcgc catccatcgc cgctctgacc tcggc
//
LOCUS       DASZAK010000296         3005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_362880, whole genome shotgun sequence.
ACCESSION   DASZAK010000296 DASZAK010000000
VERSION     DASZAK010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362880"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VGT44_05210"
     CDS             <1..663
                     /locus_tag="VGT44_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580232.1"
                     /translation="AGTNRMSATDARATATAEARGIATGSSAAALLNPYPPYGGTLVL
                     NDPLRDNSQGYNWDVNTGGCQFSAGGYLARETQQGHFQYCTARTTDFSNFAYQAQMTI
                     INGDYGGLIFRANAVTGQYYYFRIGRDGSYILIRYVDNQNIHAQVLTDGVASIVRTNL
                     NQSNLIAVVVRSSTIDLYINHRYITTVHDNTYSHGQIAVVAEDEGNSTGVLFSDVKVW
                     AL"
     gene            660..1286
                     /locus_tag="VGT44_05215"
     CDS             660..1286
                     /locus_tag="VGT44_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580233.1"
                     /translation="MSYRYQSVRADSPGTLRSEGTQLASPPAPTRLRRRSLSRVLLYY
                     ASMLLILGAAWFVGLRPYLHGLAQNQIDTQLSNVVNQLDLAQIPEISSGPLSIPVTER
                     TVNNLFVLYASPSDPVQHMHMQITPGGLRVDFQVYGFACDITGVPVVSNGQLIVANVT
                     VEGIVSLIMSPDEMTSILNAHLRDARAKLHRNVVGVILKDHEIDIMLG"
     gene            complement(1496..1846)
                     /locus_tag="VGT44_05220"
     CDS             complement(1496..1846)
                     /locus_tag="VGT44_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580234.1"
                     /translation="MNRFVTRLLSGVGVGVVTGLVVLLLRGEKTQRFLRDRWQQLRGA
                     LPAPEHVQQYAQQVANRVSQAAGEVKDAAQQAVKKVRDAGSELGDVAQQKAMPMKQTG
                     QDGSANIKQLVKHG"
     gene            2395..2805
                     /locus_tag="VGT44_05225"
     CDS             2395..2805
                     /locus_tag="VGT44_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2580235.1"
                     /translation="MPQDSAPVAGEAQAATKTILLVEDDLAIGSVLVEALAQETPYQA
                     IVATDSHAALQLVRHCTPDLLILDYGLPDLNGIELYDRLHTNEALAALPAILMTANRH
                     VPQQQLQQRQLIFCRKPFELDVFLATIETLLTAP"
BASE COUNT          672 a          833 c          774 g          726 t
ORIGIN      
        1 gccgggacca accgtatgag cgcaactgat gccagagcca ccgcgactgc tgaagccagg
       61 ggcatcgcta ccgggtcctc tgcagcagcc ttgctgaatc cctacccgcc ttatggaggg
      121 acattagtgc tcaacgaccc gctgcgtgat aatagccagg ggtataactg ggatgtgaat
      181 actggtggct gccagttctc agcgggaggt tatcttgcca gagagacaca acaaggacat
      241 ttccaatact gcactgccag gactactgac ttcagcaatt tcgcttatca ggcgcagatg
      301 acgattatca acggtgacta tggcggactt atcttccgcg ccaacgccgt gactggccaa
      361 tactattatt tccgtattgg ccgggatggg tcttacattc tcattcgtta tgtagacaat
      421 caaaatatcc atgcccaggt attgaccgat ggtgttgctt caattgttcg cacgaatctg
      481 aatcagtcta atctgattgc ggttgtggta cgtagcagca ccattgactt atacataaat
      541 caccggtaca tcaccacagt gcatgacaac acctatagcc acggtcaaat cgccgtcgtc
      601 gccgaagatg agggaaattc aaccggcgtg ttatttagcg atgtgaaggt atgggcatta
      661 tgagctatcg atatcaatct gtaagggctg actcgcctgg cactttacgg agtgaaggaa
      721 ctcagctcgc gtctcctcca gctcccactc gccttcgaag acgttctttg tcgcgcgtgc
      781 tgctttatta tgcttcgatg ctgcttatac ttggggcagc ctggttcgtc ggactgcggc
      841 cctacctgca tggattggca cagaaccaga tcgatactca attatcgaat gttgttaatc
      901 agcttgatct ggcgcagata cctgagatat cctctggccc gctgtctatc cctgtaactg
      961 agaggacggt caataatctc tttgtgctct atgcttcgcc ctctgatccg gtgcagcata
     1021 tgcatatgca gattacaccc ggcgggctgc gagtggattt ccaggtctat ggatttgcct
     1081 gtgacattac cggagtacca gtagtaagca atggtcagct tattgtagcg aatgtaacag
     1141 ttgagggcat cgtatcattg atcatgtcac ccgacgaaat gacctcgata ttgaatgctc
     1201 acttgcgcga tgccagggca aaattgcacc gtaatgttgt tggtgtcatt ctcaaagatc
     1261 acgagatcga tatcatgctc ggttagtgct atcatcgcaa aaaaggccgg atatcgatgc
     1321 aaatttccgt cttcacacat gcatggtttg taggcaatca acccaacggg agcgggtggc
     1381 gaaagcggat ttatcggcca ctacgtcacc cttccctacg ctcgtttgcc tacacctttt
     1441 tcattcacca gaggctgagc atcacattga cgctcaaccg agaacgcgcg ctcttttatc
     1501 catgctttac cagctgcttg atgttggcac tgccgtcctg gccagtttgc ttcattggca
     1561 tggccttctg ttgtgccaca tcgcccagct cacttccggc atccctcacc ttcttcaccg
     1621 cctgctgtgc cgcgtctttc acctcaccgg ctgcctgtga aacccgatta gccacttgct
     1681 gggcatactg ctggacgtgt tctggtgctg ggagagcgcc acgcagctgc tgccagcggt
     1741 cgcgcaagaa gcgctgcgtc ttttcccctc gcagaagcag gacgaccagg ccggtaacca
     1801 caccgactcc gactccactg aggagtcttg tgacaaaacg gttcatgctt caatctcctt
     1861 tctgctctga agcactggag tgtgacatgt cctctcagcc atgcggctgt tccactgtcg
     1921 gttgggacat gtcattcctc atagtcctca cacgcagcac caaagctcta cgtttcaaac
     1981 acgcctcccg accatgctag ccacttggac catgtgactt gcagtgcttc gtggcagtct
     2041 cggccagctg atggtactcc cccacaggtg gtgattcgga gccctgtaga gactgtcctc
     2101 catggaacac ttgctctcgg agcgaggaag agagtccgtc cggatctttc ttttggccct
     2161 gttcaccaaa ggtggggagt tccagatcgt aggccgtatt gacatccctt caagctcgtc
     2221 gcacgagggt agcactgatt gctcgcggtt gcttcgccca gcagcgatgc gctcgtttca
     2281 cgtaccgtgg tgtgcttcca gtggatggag tacgcctctg aaaccgtttg cttgtctctc
     2341 tcgtgttaga aagagagcac atttggtgtc aaccgaaaga gtgagagcac gactatgcct
     2401 caagatagcg caccggtagc gggagaagcg caggcggcca ccaaaacgat cctcctcgtc
     2461 gaggacgatt tggccattgg ctcagtcctg gtggaggctc ttgcgcagga gacaccctac
     2521 caggcgatcg tggcaactga tagccatgcg gcgttgcaac tcgtgcggca ctgcacaccc
     2581 gacctcctca ttctggatta cgggttgccg gatctgaacg gcatcgaact ctacgaccgc
     2641 cttcacacca acgaagcgtt agcagctctc cccgcgatct tgatgaccgc caatcgccat
     2701 gtccctcaac agcagctaca acaacgccag ctcatcttct gcaggaaacc atttgaactg
     2761 gatgtgttcc tcgcgacgat cgaaacgctc ctcacggcac cgtgagcgat gggggaaaca
     2821 agctcgtcat ctcggatggg aggccagtcg tgttagcaaa aagaaagggt aaagaggagt
     2881 gctatgacta tgccagtgca ccagcaagga aacaatgcaa acacagcagt agcgagaaca
     2941 gccgtcgtgc ggttgcttca ggaaggaggg agtcccgcaa agttggatgt cagcggtcag
     3001 gattt
//
LOCUS       DASZAK010000297         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_42535, whole genome shotgun sequence.
ACCESSION   DASZAK010000297 DASZAK010000000
VERSION     DASZAK010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42535"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1063)
                     /locus_tag="VGT44_05230"
     CDS             complement(71..1063)
                     /locus_tag="VGT44_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2580236.1"
                     /translation="MMFRRILVPLDGSDRAEEAIPVAARLAHAANGTVILVQVAPFSL
                     DFQADKKQPAEMYPDNVIEEVKALAANYLYNASKKPEMVGVNTETRVEYGEAAQSILE
                     AVDPLRVDLIVMCSHGYSGFKRWALGSVAQKIIPHSPVPVLVLRDGGPMLAAKPLDAL
                     VSLDGSPLAESALAPVTQLLAALAPQAQKTLHLVRVVALPVTYGRFATNAPFDQLREE
                     AKRAAHQYLAAVAEQLIEAGGAVSDLTVTTSIAVNPDVAEALIQTTEQKLHSGGRFDL
                     VAMATHGRGGLQRLMMGSMTERVLHATKLPMLIVHSAGQIKQAHERKEQIGTTR"
     gene            complement(1167..2204)
                     /locus_tag="VGT44_05235"
     CDS             complement(1167..2204)
                     /locus_tag="VGT44_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2580237.1"
                     /translation="MFERILVPLDGSKASERAIPVAARIAHATGGTIVFVEVVLPPIE
                     FGTYTADRTIPLKPGAFERREGAAAHYLTSIPETYAGELAGIKTELDLSVGAASPEIY
                     EAARLERIDLIVLCSHGETGLKRWAFGSIAQEAVRHSPAPVLVLNEHGIIPPVPEYAQ
                     PLRILVPLDGSPFAEAAILPAANLVAALSTSPHDELHLLRVVVLPTVYRKMKSQVYHS
                     DLMLDDIKRDAEKYMKSVAEHCEKDLAGFNLQVSTSVVVNPDVTGTIVREAAQARDVA
                     QSTGYDLVAMATHGRSGLQRLMMGSVADHILGNTKHPLLIVRPHAEAKGKETGEAVQT
                     EVEVQSWVGLL"
BASE COUNT          477 a          714 c          673 g          559 t
ORIGIN      
        1 taagaccttt gagtgtatac tatcgcaagg gggtcgcttg gaaagcgacc cccttctctt
       61 tgttggcgcg ctagcgggta gtcccgatct gctctttgcg ctcatgtgct tgtttgattt
      121 gcccggctga gtgaacaatc agcatgggga gtttcgtggc gtgcagtacg cgctcggtca
      181 tactgcccat catcaagcgt tgcaggccgc cgcgcccatg cgtcgccatc gcgaccaggt
      241 caaagcgtcc gccgctgtgc aatttctgtt ctgtcgtctg tatcagggct tcggctacat
      301 cgggattgac ggcaatcgac gtagtcaccg tgagatcgga gactgcccca ccggcctcga
      361 tgagctgctc cgcaacggct gccaggtact gatgcgctgc gcgtttcgct tcttctctca
      421 actggtcaaa aggcgcattc gttgcgaatc taccgtaggt cacaggcaga gcaaccacac
      481 gcaccaggtg cagtgttttt tgcgcctggg gggcgagtgc cgcgagcagc tgggttacgg
      541 gggcaagcgc gctttcggcc aatggcgatc catccaggga taccagtgca tctagcggtt
      601 tcgcggcaag catcggaccg ccatcacgta agaccaggac cggcaccggg ctatgcggga
      661 tgatcttctg cgcgacgctg ccaagcgccc agcgtttgaa accgctgtag ccatggctgc
      721 acattacgat caagtcgacg cgcagcggat caactgcctc gagaatcgac tgcgcggctt
      781 ctccatactc cacgcgggtt tcagtattta cgccgaccat ttctggcttc ttgctggcgt
      841 tgtagaggta gttggccgct agagccttga cttcctcaat cacattgtcg gggtacatct
      901 ccgcaggttg ctttttatcc gcttgaaaat caagggaaaa cggggcaact tgcaccagaa
      961 tcactgtgcc attggcagca tgtgcaaggc gagcagcgac agggatggcc tcttcagctc
     1021 tgtccgaccc atccagcgga acgagaatac gtctaaacat catgctcctc cttcttcgcc
     1081 aggccacccc ttgcggagcg gcccggcctt tctctagacg catgagcagg tatggttatg
     1141 aacgggcgag tacgttgatc gctctgctag agcaatccta cccagctttg aacctcgacc
     1201 tccgtctgca cagcttcacc tgtttctttc cctttggctt cggcgtgcgg tcgcacaatc
     1261 aataacggat gctttgtgtt gccgagtatg tggtctgcca cgctgcccat catcagccgc
     1321 tgcaagccgc tgcgtccatg cgtcgccatg gcgaccaaat cataaccggt ggattgtgca
     1381 acgtctctgg cctgcgctgc ctcacggaca atagttcctg tcacatcggg attgaccacg
     1441 acggacgtac tcacctggag gttgaagccc gcgagatcct tttcacagtg ttcggcgacg
     1501 gacttcatgt acttttcagc atcacgcttg atatcatcca gcatgaggtc actgtgatac
     1561 acctggctct tcattttcct gtataccgtg ggaagaacaa ccacgcgcag gagatgcagc
     1621 tcgtcatgtg gagacgtgga aagcgcagcc acaaggttgg cggctggcag gatagcagcc
     1681 tcagcgaacg gggagccatc cagtggcaca aggatacgca gaggctgagc gtattccggt
     1741 accgggggaa ttatcccgtg ttcgttgagg accagcaccg gggcggggct gtgacggacg
     1801 gcttcctgag caatgcttcc gaacgcccag cgtttcagtc ccgtctcacc atgactgcat
     1861 aggacgatca gatcgatcct ctccaggcgc gctgcctcgt aaatctccgg tgatgctgcg
     1921 ccgaccgaca agtccagttc cgttttgata cccgccagtt caccggcata cgtttcgggt
     1981 atgctggtca ggtagtgggc tgctgccccc tcgcgccgct cgaacgcgcc tggcttgagc
     2041 ggaattgtcc ggtcggccgt ataagtcccg aactcgatgg gaggaaggac aacttctaca
     2101 aagacgatcg ttccgccggt ggcatgtgcg atgcgcgcgg cgacagggat agccctctcg
     2161 gatgctttcg agccatccag gggcaccaaa atccgctcaa acatctgctc gtactccttt
     2221 cgacatgctt gtcgatatct aacagctgcg ctgctttctt tctggatcaa gcatatccct
     2281 gaaagctcac gaaggcatca ccgtacagtg aatatatctc acagtttggt aacagccact
     2341 gctcttttaa tcctgatgaa cttttccaac cgtaaccggt gatcgtttcc gtaggggcga
     2401 gcctttctgg tcgcccgccc gcg
//
LOCUS       DASZAK010000298         5398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_42545, whole genome shotgun sequence.
ACCESSION   DASZAK010000298 DASZAK010000000
VERSION     DASZAK010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42545"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VGT44_05240"
     CDS             <1..290
                     /locus_tag="VGT44_05240"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907707.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEV2580238.1"
                     /translation="CRVAIDKLPGGPIGTRTPIALRPPRGETYFAIESSKGELGIYFI
                     SDGSEYPWRAKIRGPSFVNLQILPEILRGHKMSDVIAIMGSIDIVLGEVDR"
     gene            304..1689
                     /locus_tag="VGT44_05245"
     CDS             304..1689
                     /locus_tag="VGT44_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEV2580239.1"
                     /translation="MFVASILVRNAATFQFDANFWLQILQFVIAFLSFFGFVLTSAII
                     LILAERKVMGWMQDRVGPIHTGPWGLLQTVADVGKLLMKEDIHAGLTDKTLFILAPSV
                     FLAPVIAAFAVLPFSPYVGLPGTALATGIVYYVAMSSIDIVGIVMAGWSSNNKYSLIG
                     GLRSAAQMISYELPLVLALVSVVMLTSVLANNGLGTISIRDIQNYQSASLWPGKGIGF
                     FDFIFGGDTPWAWFFLVQPLMLIIYYTCGLAETNRAPFDLPEAESELVAGYLTEYSGM
                     RWALFFLGEYGNMTIVSSIATYIFLGGFSGPGVSFLTALNSFGWALLGNLLAVGYFIV
                     KVYLLCLVFIWVRSTLPRLRADQLMQFAWLILIPTTLLNIIITALIYLVMNSLGAPNW
                     LFLVVLGIVNWAMLFVFIRIVSRATVATTRRAQAPAIRARRRAQALPAATTATAAQLP
                     ERAGAVGGAQQ"
     gene            complement(2323..3153)
                     /locus_tag="VGT44_05250"
     CDS             complement(2323..3153)
                     /locus_tag="VGT44_05250"
                     /inference="COORDINATES: protein motif:HMM:NF013729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEV2580240.1"
                     /translation="MTDNSIGVFISYAHEDEQLYNKFETHFTLLKHQNLIATWQYRDI
                     SAGKEWSKQIGEYLAKAQLILLLVSSDFMASEYCYGIEMKQALQRHEQGDANVIPVLM
                     RPVDLEGAPFEKLVMLPTNLIPVTKWKNRDEAFVNIVKGIRKAIQDWNKPYTFLSPQE
                     KVSMNFQQTVQSPVELEKKYLSQFQDRLNKALSQLSSVDPNHLWSPEEVGSMISYLQS
                     LERALERDYGSLAQQLSASGLPQLSHRMQATRLDIHTALGIYSQMYQKEAYILAKAAS
                     "
     gene            complement(3245..3991)
                     /locus_tag="VGT44_05255"
     CDS             complement(3245..3991)
                     /locus_tag="VGT44_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV2580241.1"
                     /translation="MKHATEQMNTNFWPIVRIIELTTALSTGLAIWRAYGLWATMRYI
                     AGLALAMKVQRTFLAAMIRKTGVEQVSVADVMTLSRGATGAVLVGLVASGIRDRTGLA
                     GRLGWSLILLEATVIDWLDGPLARKLGSSRLGAVMDIEADSWLTLWSGISAVSWGNLP
                     RWCLLPPIMRYLDPLLDLSRGRLPHGGGPWWSRLTGTAQMVLFLAALAPFERRWWKRV
                     MGVVKVAALPVSGGQGAAVVALLVKKVREE"
     gene            4063..4728
                     /locus_tag="VGT44_05260"
     CDS             4063..4728
                     /locus_tag="VGT44_05260"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEV2580242.1"
                     /translation="MIQAPYGRNIIPAHHNRRDRQAAWCARFAIVGVFYFITAVVILH
                     PLRPDLNPVTHAVSNYANGPFGFLMTSAFFVLAISEFALAWGLARCLSLTRRTMISVA
                     LLGLTGSGLIVTGLFRSDVNVPRPPASASAIVHWIGAGGSFLSLMIAIFLLSGYFKKD
                     APWQSFSRLSYSWLAAIVLALAAYGTLSLLSWTGIGERIYIATSVVWLLLASLRLRTV
                     TTS"
     gene            4743..>5398
                     /locus_tag="VGT44_05265"
     CDS             4743..>5398
                     /locus_tag="VGT44_05265"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV2580243.1"
                     /translation="MSNVVNINTGVATGEVAGFYDSRFERVADEFVRNFQQRGEVGAS
                     VSITLDGEPVVDLWGGSANPETGVPWKKDTLTLVWSCSKGATALCAHMLADRGLLDLD
                     APVARYWPEFAQAGKENIPVKMLLNHQSGLAAFREPLPPGAFYDWDLAVSMLAAQEPF
                     WKPGSMHGYHGFTFGWLVGEEVRRVSGKSLGTFFREEVAEPLGLDFWIGLPEEMEGRV
                     A"
BASE COUNT         1120 a         1628 c         1411 g         1239 t
ORIGIN      
        1 tctgccgcgt cgccatcgac aaactacccg gtggccccat cggtacacgc acaccgattg
       61 ctctacgtcc gccgcgcggc gaaacttact tcgccatcga aagtagcaaa ggtgagcttg
      121 gtatctactt catcagcgac ggcagcgagt atccctggcg cgccaagatt cgcggcccat
      181 cctttgtcaa tctgcaaatc cttcccgaaa tcctacgcgg acacaaaatg agcgacgtta
      241 tcgccatcat gggcagcatc gacattgtgc taggtgaagt tgaccgttaa acggagttat
      301 catatgttcg tagcaagtat attggtgaga aacgcagcga cctttcaatt cgacgcaaac
      361 ttctggttgc agattttgca gttcgtgatc gccttcctct cattcttcgg ctttgtgctg
      421 accagcgcca taatcctgat cctggccgag cgcaaggtca tgggctggat gcaggaccgc
      481 gtcggcccca tacacaccgg cccctggggg ctgcttcaga cggtggccga cgtgggcaag
      541 ctgctcatga aagaagacat ccacgccggg ctaaccgata agacgctctt catcctggcc
      601 ccgtccgttt tcctggcgcc cgtcatcgct gcctttgccg tcctgccttt ctccccctac
      661 gtcggtctcc ccggcaccgc gctcgccacc ggcatcgtct actacgtcgc catgagttcc
      721 atcgacatcg tcggcattgt catggcgggc tggagttcca acaacaaata ctcgctgatc
      781 ggcggcctgc gttccgccgc gcagatgatt tcctacgaac tgccgctcgt gctggcgctg
      841 gtgagcgttg tcatgttgac cagcgtgctt gccaacaacg gacttggcac catttctatc
      901 cgtgacatcc agaactacca gtcagcgagt ctctggccgg gcaagggcat cggcttcttc
      961 gacttcatct tcggtggcga taccccctgg gcctggttct tcctcgtgca gccgctcatg
     1021 ctgattatct actacacctg tgggctggcc gagacgaacc gcgcgccctt cgacctgccc
     1081 gaagccgaat ccgaactggt cgcgggctac ctgaccgagt acagcggaat gcgctgggcc
     1141 ttgttcttcc tgggcgagta tggcaacatg acgattgtct catcgattgc tacctacatc
     1201 ttcctgggcg gcttctccgg ccctggcgtg tcattcctga cagccctgaa cagcttcggc
     1261 tgggctttat tgggcaacct gctcgccgta ggctacttca tcgtcaaggt ctacctgctc
     1321 tgcctcgtct tcatctgggt gcgcagcaca ctgccacgcc tgcgcgccga ccagttgatg
     1381 caattcgcct ggctgatcct gatcccgacc acgctgctca acatcatcat caccgccctg
     1441 atctacctgg tgatgaatag cctgggcgcg ccaaactggc tcttcctggt cgttctgggc
     1501 atcgtcaact gggccatgct cttcgtcttc atccgcatcg tcagccgcgc caccgtcgcc
     1561 accacgcgcc gcgcgcaagc gcccgccatc cgcgcccgcc gtcgcgcgca ggccctcccc
     1621 gcagccacaa ccgccaccgc tgcgcaacta cccgaacgcg ccggagccgt gggaggcgcg
     1681 cagcaataga gacacgatga cgcgcccaga caatttaatg cgaacattgg cgttcaaacc
     1741 ccagggcgac cgcaagggtc tccctcgtcc tccctactct gacgtatatc aagcgcagca
     1801 aagctcatac gagctcagac gtgcttttcg gccccttcac aagacgagca agagagacat
     1861 accgttgccc ctgcgtaaaa gcagttcgtc ttccttcccc cctagtcagg tcattctgga
     1921 tatcattggg aaggatgagc gtatcaggtg aggcaggcag cgtgagaaag acaaccttga
     1981 gttgattttt accccattcc tcgttccttg caggaatcat tcgcttctcc aacgctctta
     2041 gccacgctct atggagatca gataggtttg ttctagtctc gatttcgtcg cgagatatag
     2101 cctcaactgt ccccaggatt tttggaacca ggtggtagat tttgttgtct gtgtaaaaag
     2161 ccatgcgaac actatcctga atagaacggt tgggctggca aacgtaggca gaacagcgta
     2221 gatactcctc ccacgcgaat cccgccgcga caacaacgac tcgatcagat gcttcgctta
     2281 tcaccataca atcaataagc tccttctttg tgaacattct gtttagctcg ctgctttagc
     2341 caggatgtat gcctcttttt gatacatttg agagtaaata cccaacgcgg tatgaatatc
     2401 tagccgtgtt gcttgcatcc tatgggatag ctggggtagg cccgacgcgc ttaactgctg
     2461 cgccagagaa ccatagtctc gctctagcgc tctctccagg ctctggagat aagaaatcat
     2521 agaacctacc tcttcgggag accagagatg attcggatcg acagatgaaa gttgtgacaa
     2581 agccttgttg agacggtcct ggaactgaga aagatacttt ttttctagct ccacggggct
     2641 ttgtactgtc tgttggaaat tcatggatac tttttcttga ggagaaagga aagtgtatgg
     2701 cttattccag tcttgaattg ctttccgaat acctttgact atgttgacaa atgcctcgtc
     2761 gcggttcttc cacttcgtga cggggataag atttgtcggc aacatcacca gtttttcgaa
     2821 aggcgcgcct tctaaatcga cagggcgcat caggacaggt ataacgtttg catctccctg
     2881 ctcatgcctt tgtaaagctt gtttcatttc gatgccatag caatactcgg aagccatgaa
     2941 atctgagcta accagtaaaa ggattaattg agctttcgct aaatattcac caatctgctt
     3001 tgaccattct tttcccgcgc ttatatcgcg gtattgccaa gtagcaatca ggttctgatg
     3061 ctttaaaagt gtgaaatgtg tttcaaattt gttgtacagt tgctcgtctt catgcgcgta
     3121 ggagataaag acaccaatgg agttatctgt catttattca gaccgtctct tttcgtaccc
     3181 gccgcgaaat ggctttctca tgtagagaag ataaagcaag cacagctaat tgtcaagaag
     3241 ttgcctactc ctcccgcacc ttcttcacca gcagcgccac caccgccgcg ccctgcccgc
     3301 cactcaccgg cagcgccgcc actttcacaa cccccatcac cctcttccac catcgcctct
     3361 cgaagggagc aagcgcggcc aggaacagca ccatctgggc tgtgccggtc agccgactcc
     3421 accaggggcc gccgccatgc ggtagcctgc cccggctcag gtcgagcagc gggtcaaggt
     3481 agcgcatgat gggaggcaat aggcaccagc gcggaaggtt gccccacgag accgcgctga
     3541 tgcccgacca gagcgtcagc caggaatcgg cctcgatatc catgaccgct cccaggcgtg
     3601 aagagccaag tttgcgcgca agcggcccat ccagccaatc aataacagtc gcttcgagca
     3661 ggatcagcga ccagccaagc cgaccggcca gccccgtgcg gtcgcggatg cccgatgcca
     3721 ccaatcccac cagtaccgcg cccgtcgcgc cccggctcaa cgtcatcacg tcggctaccg
     3781 aaacctgttc caccccagtt ttacgaatca tggctgccag gaatgttcgc tgtaccttca
     3841 tcgccagcgc cagacctgct atatagcgca tcgtcgccca caagccgtaa gcgcgccaga
     3901 tagccagccc tgtcgatagc gctgtggtca gttcgataat tctcacgatt ggccagaagt
     3961 tcgtattcat ttgttcggta gcgtgtttca tgtgtgataa gcgtatggta caatagatca
     4021 cgttgttgag taacgcaaag ccgcgttcga ggtgagacgc tgatgatcca ggcaccctac
     4081 ggacggaata taatacccgc acatcacaat cgccgtgaca ggcaggcagc ctggtgcgcg
     4141 cgcttcgcaa tcgtaggtgt gttttatttt atcacagccg tagtgattct acatccactt
     4201 cgacccgatt tgaaccccgt cacccacgcg gtgagcaact acgcgaacgg cccattcgga
     4261 ttcttaatga cctccgcctt cttcgtcctg gcaatcagcg agtttgccct ggcctggggg
     4321 cttgcccgct gcctctcact cactcgaaga acgatgatca gcgtcgcgct actgggtctg
     4381 acggggtcag ggctaatagt gacgggactc tttcgcagcg atgtgaatgt gccgcgcccg
     4441 cccgcctccg cgagcgcgat agttcactgg attggcgcgg ggggttcctt cctgagcctg
     4501 atgatcgcta tcttcctcct atcgggctat ttcaagaagg acgcgccctg gcaatcgttc
     4561 tctcgcctgt cctatagctg gctggcagct atcgtgctgg cattagcagc atacggcaca
     4621 ctgtcactcc tgagctggac aggcatcggc gaacgcatct acatcgcgac cagcgtagtg
     4681 tggctcctgt tagcgtctct acgactccga accgttacaa ccagttagaa agggaaccgc
     4741 tcatgtccaa tgtcgtcaac atcaatacgg gtgtcgccac cggcgaggtc gccggattct
     4801 acgattcgcg ctttgagcga gtagccgacg aattcgtgcg caactttcag cagcgcggcg
     4861 aagtgggcgc ctcggtcagc atcacacttg atggcgagcc ggtcgtagac ctgtggggag
     4921 gaagcgccaa cccggaaacc ggtgttccct ggaagaaaga taccctgacc ctggtctggt
     4981 cgtgttccaa aggcgcgacc gcgctctgcg cccatatgct tgctgatcgc gggctgctcg
     5041 acctggatgc gccggtggcg cgttattggc cggagttcgc tcaggcaggc aaggagaaca
     5101 tccctgtcaa aatgctgctc aatcaccaga gcggcctggc agcatttcgc gaaccgttgc
     5161 cgccaggagc attctacgat tgggatctcg ctgtcagtat gctggcggcg caggagccat
     5221 tctggaagcc cggtagtatg catggttatc atggcttcac cttcggctgg ctggtcggag
     5281 aagaggtgcg cagggtttca ggcaaatcgc tggggacgtt cttccgcgag gaggtagcgg
     5341 agccgctcgg cctcgatttt tggatcggcc tgccggagga aatggagggt cgcgtagc
//
LOCUS       DASZAK010000299         1813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_575625, whole genome shotgun sequence.
ACCESSION   DASZAK010000299 DASZAK010000000
VERSION     DASZAK010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575625"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="VGT44_05270"
     CDS             <1..803
                     /locus_tag="VGT44_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEV2580244.1"
                     /translation="KVLQMQKARNEAQLVEELARAGNKKNGELIDTSLSKKFLRCNTN
                     DPTRSELFIVEGDSAGGSAGQGRFSEFQAVLKLKGKPLNVAKADLKRIVENEEIRTII
                     NVLGTGTRDSFDISRLKFERVIIATDADVDGLHIQCLLLTLFHQEFSDLIEDGYVYIA
                     CPPLYSIKYKGKVIWLLDDEARQQFIKTHADAQGLEFKRFKGLGEMNPKELRETMFDP
                     ARRTLRRVTIEDGVLAARLVAQLMEDENAEARRTFLAEHARKIKELDV"
     gene            807..>1813
                     /locus_tag="VGT44_05275"
     CDS             807..>1813
                     /locus_tag="VGT44_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV2580245.1"
                     /translation="MARTTDNTTEAGIIREEDFAVIMAQAFATYGLSVVTDRALPDAR
                     DGLKPVHRRILAGMREARYLSSRPTVKSAEVVGLILGNYHPHGDASVYDTAVRMAQPF
                     SMRYPLIEGQGNMGSEDGDAPAAYRYTEMRLSPLAEALMADMERDTVPLHPTYKQDPK
                     VVEPDYLPGRIPPVVNPSSGIAVGLSTNIPPHNLTEVLRACIALLDRPNMSIEQLMAY
                     IQGPDFSQGGRIVGVDGVRDYLTTGKGRVIVRAEVRLEETPRSRSLIVTQIPPIGRDK
                     VKASIVKAINARKLEGLMPDLRDESDTEKGTRIVLDLRKDADAAQVLSQLFSETDLQV
                     S"
BASE COUNT          430 a          502 c          502 g          379 t
ORIGIN      
        1 agaaagtcct tcagatgcag aaggcgcgta acgaagcgca actggtagag gagctggcac
       61 gggcaggtaa taagaaaaat ggcgagttga tcgacacctc gctctcgaaa aagtttctgc
      121 gctgcaacac taatgaccct acccgctcgg agcttttcat cgtcgaaggt gactctgccg
      181 gtggtagcgc tggccaggga cgcttctcgg agtttcaagc agtgctcaag ctcaagggca
      241 agccgctcaa tgtagcgaaa gccgatttga agcgcatcgt cgagaacgag gagatccgca
      301 ctataatcaa tgtattaggc acgggcaccc gcgactcctt tgacatcagc cgtctcaagt
      361 tcgagcgtgt aatcatcgcg accgatgctg acgtggatgg gctgcatata cagtgcctcc
      421 tgctcacgtt gtttcatcaa gaattcagcg acttgatcga agacgggtat gtctatattg
      481 cttgcccgcc gctgtactct atcaaatata agggcaaagt catctggctg ctggatgatg
      541 aggcgcggca acaatttatc aagacccatg ccgatgctca gggattggaa ttcaaacgtt
      601 tcaaaggtct aggtgagatg aacccgaaag aactgcgcga aaccatgttt gatcccgctc
      661 gtcgtacgct caggcgtgtg acgatagagg atggcgtact ggcagcacgc ctggtggcgc
      721 aactgatgga agatgagaac gcggaggctc gccgtacctt cctggcggag catgcccgta
      781 aaattaaaga actggacgtt tagcacatgg ctcgcacaac ggacaacaca accgaagccg
      841 gtatcattcg cgaagaagat tttgccgtca tcatggctca agccttcgcc acctatggcc
      901 tctctgtcgt cacagatcgt gcactaccag atgcccgtga cggcttgaag ccagtgcatc
      961 ggcgtatact cgcaggcatg cgtgaggctc gctatctttc cagccgccca actgtcaaaa
     1021 gtgccgaagt ggttggcctc atccttggca actatcatcc ccatggcgac gcatcggtct
     1081 atgatactgc cgtacgcatg gcccagccgt tcagcatgcg ttatccgttg attgaaggac
     1141 agggaaacat gggtagcgag gatggtgacg cccccgctgc ctatcgctat acagagatgc
     1201 gcctctcacc gctagccgaa gcactgatgg ccgatatgga acgcgacacc gtgccgcttc
     1261 atcccacgta caagcaggac ccaaaggtgg tcgagccaga ttacctgcca ggacgcatcc
     1321 cgcccgtcgt caatccttcc agtggtatcg ctgttggcct ctcgaccaac attccgccgc
     1381 ataatctgac cgaagtcctg cgtgcctgta tcgctttgct tgatcgcccc aacatgagca
     1441 ttgagcaact gatggcctac atccagggac cagatttctc ccagggtgga cgcatcgtgg
     1501 gcgttgatgg tgtcagagac tacctgacga ccggtaaggg acgggtcatt gtgcgagcag
     1561 aggtacgcct ggaagagacc ccccgcagcc gctcgctgat cgtaacacag attcccccaa
     1621 ttggcaggga taaggtgaag gcgtccatcg tcaaggccat caacgcgcgc aaactcgaag
     1681 ggctgatgcc cgacctgcgc gatgagagcg ataccgagaa gggcacgcgt atcgtgctgg
     1741 atctgcgtaa agatgctgat gcggcccagg ttctctcgca gctctttagc gaaacagatt
     1801 tgcaggtctc ctt
//
LOCUS       DASZAK010000300         3856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_42568, whole genome shotgun sequence.
ACCESSION   DASZAK010000300 DASZAK010000000
VERSION     DASZAK010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42568"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1589)
                     /locus_tag="VGT44_05280"
     CDS             complement(<1..1589)
                     /locus_tag="VGT44_05280"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2580246.1"
                     /translation="MALPPNKPIVCPVLIGRAADLTALHVLVDQAKRGEGQVALISGE
                     AGIGKSRLVTEAKTYAASQGFLLLQGSCFQADRAFPYAPLLDLLRSYFSGSSLVTGQN
                     DLTPFAQELSQFLPDVTPLIPDSTPLMVPPPLDPQQEKRRLFAMSLHFVTKLSARQQI
                     VFIVEDLHWSDDSSLELLLYLARGCTHLPILFVLTYRSDEVSPELRHYLAEFDRMHLA
                     QDFSLRRLARADIDAMLQAIFAMHEAVHTGLAESVYALTEGNPFFVEEVLKSLIATGE
                     IASKDGVWERTLLFGTHTRSPSVPRSVQDAVYQRTKQLSAPARQVLALAAVAGRRFDL
                     TILEQVMETDESHLLALMKELVAAQFVTEEASDQFSFRHALTRQAIYSELLAGERRAL
                     HRSIADTIEQHIFPSSILDAQLVDLAYHFYEGEVWSKAAEYGQHAGERALILYAPRAA
                     IEHFTRTLAALKRLGNTPSAAVVRARGQAYETIGAFEQARSDYEQALSIAQQTQDGTM
                     EWQSLLDIGCLWAGRDYAQAG"
     gene            complement(1860..1933)
                     /locus_tag="VGT44_05285"
     tRNA            complement(1860..1933)
                     /locus_tag="VGT44_05285"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1897..1899),aa:His,seq:gtg)
     gene            complement(1979..3796)
                     /locus_tag="VGT44_05290"
     CDS             complement(1979..3796)
                     /locus_tag="VGT44_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV2580247.1"
                     /translation="MVAQDIAQLPAAKYWHDPILIVGANPVGLITALGLARYKIPSVV
                     LDEGEGVSLESNQVALLDATTLSILDAWENGLGQFVASQGLIPAVERVFLRASQLYSA
                     ALPAHLPGSGYPQQVTLHHAALEQLLLQALQQIGGCQVLWQHRVKGFTHDHSGIDVEL
                     ESPAGAKFLRAPYLLVTSDTSNAPDAAPGANQTVTTGSLAPVAQSANKPSKHDERYLS
                     VDVLTELESVGEQWFWLDAPFNPGHITQLFSLPGGLVRILYQLGPRDDSGQQAEALQR
                     RIEATLRGHPFEVIAISSHTRKRGVIEQFKEKRTLFLGSAAHHVALFGASEVNSGALD
                     AWNLVWKLALVRAGLAMQDLLETYHLERHRAVIDYMKRTKETLAFVAPTSGLATWKRD
                     TTLRLSHSFKSMRDSMRLSEARGASGSIAQADSPIFSDDNRLYLGGRFTRLTAEQNST
                     LKRFRQGPRVGMLAPSLALPDADTGAMTPLLGRVSSGFLALCFTNDIDMAMNMLRRIP
                     VEMSGVPILFYLITPTIPAASAEDGITMLVDAQGRVARAYNAGQRSLYLVRPDGVIAA
                     RRFDSDFHDIPALLRHAIGEDVVDSQVHIPRPEMASSGS"
BASE COUNT          860 a         1062 c         1128 g          806 t
ORIGIN      
        1 tgtcccgcct gagcatagtc gcgccctgcc cacaggcatc cgatatcgag caaactctgc
       61 cattccatgg tgccgtcctg tgtctgttgg gcaattgaga gggcttgttc gtaatcgctg
      121 cgtgcctgct caaacgcgcc gattgtctca taggcctgcc ccctggcccg taccactgct
      181 gcggatggag tgttgcccag gcgcttgagc gccgccaatg ttcgcgtaaa gtgctcaatt
      241 gccgcacggg gcgcgtacag gatcaacgcc cgttctccgg cgtgttgtcc atactccgcg
      301 gcctttgacc agacttctcc ttcgtagaaa tgatacgcga ggtcgaccag ctgagcatcc
      361 aggatcgagg aggggaagat gtgctgctcg atcgtgtcgg caatactacg atgcaacgct
      421 ctgcgttcgc ctgcaagaag ttctgaatag atggcctgcc gtgtgagtgc gtgacggaaa
      481 gaaaactggt cggaagcttc ttccgtcacg aattgggctg ccaccaattc tttcatcaag
      541 gcgagcaggt gggactcgtc tgtctccatc acctgctcaa ggatagtgag gtcgaaacgt
      601 ctgcctgcca ccgccgccag agccaagacc tgcctggccg gagcgctcaa ctgttttgtc
      661 cgttggtaaa cggcatcctg cacgctgcgt ggaactgagg gagagcgtgt gtgcgttccg
      721 aagagcaagg tgcgttccca gactccatct ttggaggcga tttcgccagt cgcgatcagc
      781 gatttcagca cctcctcaac gaagaaaggg tttccctcgg tgagcgcgta cacgctttct
      841 gccaaaccag tatgcactgc ctcgtgcatg gcaaagatgg cctggagcat tgcatcaatg
      901 tcagcacgag cgagtcgcct caaggagaag tcctgggcaa ggtgcatccg gtcaaattcg
      961 gctaagtaat gcctcaactc tggagacact tcatcactgc ggtaggttag cacaaaaagg
     1021 ataggcagat gggtacatcc ccttgccagg tacagcagga gttcaaggct gctgtcgtcg
     1081 ctccagtgga gatcttcgac gatgaacact atctgttggc gtgcggacag tttcgtaaca
     1141 aaatgcaacg acatggcaaa aagacggcgc ttctcctgtt gtggatcaag tgggggtggt
     1201 accatgagag gtgtcgaatc ggggatgaga ggtgtcacat cagggagaaa ctgtgaaagt
     1261 tcttgcgcaa acggtgtcag gtcgttctgc cctgtcacaa gtgaagaacc agaaaagtaa
     1321 gaacgcaaca aatcaagcaa gggggcatat ggaaacgcac gatctgcctg aaagcaactc
     1381 ccctggagca gcaaaaagcc ctggctggcg gcatacgttt tcgcctctgt taccaggcgc
     1441 gatttgccaa tgcctgcctc accactaatc agggcaacct gcccctcccc tcgttttgcc
     1501 tggtcaacga gtacatgaag ggctgtcagg tccgccgcac gaccaatcaa gaccgggcaa
     1561 acgattggct tattcggggg tagcgccatg gctttcacga ccttttgatt aaatttaaca
     1621 catgcgctct taagataata gtcattttaa caggaaaaca ttgtgggtgg aagagtaaag
     1681 catgatagtt gacccgatag ttcaggaagg tttctcaatt agatttgcgg ctagaggacg
     1741 caatttggac gaaggctaag aagcagtagc aaagtttgtc ctcaaaatat agaaacaaga
     1801 atatccgaaa tggccaaata tagataaagt cataggtgga aaagagcgca taaaaaaggg
     1861 gtgaccgatg gggcttgaac ccacaaccgc tggagccaca atccagagct ctgcctaatt
     1921 gagctacggt caccacgcgt tgaacgtaac gccagtatag aggacgctat gtggtttgtc
     1981 aagagccgga ggaggccatt tcggggcgtg ggatgtggac ctgcgaatcg accacatcct
     2041 cgccgatggc atgacgcagc agggccggga tatcgtggaa gtcgctgtcg aagcggcgcg
     2101 ccgcaatcac gccgtccggt cgtaccaggt atagcgagcg ctgcccggca ttatacgccc
     2161 tggccaccct gccctgcgca tcgaccagca tcgtgatacc atcttcagcg gacgcggcgg
     2221 gtatagtggg tgtaatgaga tagaacagga tgggtacgcc ggacatctcc accggaatgc
     2281 gccgcagcat attcatcgcc atgtcgatat cgttcgtgaa gcacagcgcg agaaagcccg
     2341 aactgacgcg gcccagcagc ggcgtcattg cgcccgtgtc ggcgtccggc agcgccagcg
     2401 aaggagcaag cattccaacc ctcggcccct gccggaagcg cttcaaggtc gagttctgct
     2461 cggcggtcag gcgagtgaaa cgcccgccca ggtagagccg gttgtcgtcc gagaaaatgg
     2521 gtgaatccgc ctgggcaatc gacccgctcg cgccgcgcgc ttcgctcaag cgcatcgaat
     2581 ctcgcataga cttgaatgaa tggctcaggc gcagcgtcgt atcgcgtttc caggtcgcca
     2641 atcccgaagt aggggcgacg aacgcgagtg tttccttagt gcgcttcata tagtcaataa
     2701 cggctcgatg tcgttcaagg tgataggtct caagtaggtc ttgcattgcc agtccagcgc
     2761 gcaccagcgc caatttccag accaggttcc aggcatcgag cgcgccgcta ttgacttcgc
     2821 tggctccgaa tagtgcgacg tgatgcgccg cgctgccgag aaacagcgtg cgcttctcct
     2881 tgaactgctc gatcacccca cgtttacgtg tgtggctgct gatggcgatc acctcgaagg
     2941 gatgccctct tagcgtggcc tcgatgcgtc gctgcagcgc ctctgcctgc tggcctgagt
     3001 cgtcgcgcgg cccaagctgg tagaggatgc gcacgaggcc gccgggcagc gagaaaagct
     3061 gcgtgatgtg gccggggtta aacggcgcat ccagccagaa ccattgttcg cccacgctct
     3121 ccaactctgt cagcacatcg acactcaaat atcgctcatc gtgtttgctt ggcttgttcg
     3181 cagactgagc caccggcgca agcgagccgg ttgtcaccgt ctggttcgct ccaggcgcag
     3241 cgtccggcgc gttcgaagtg tcggatgtga cgagcagata cggggcgcgc aggaactttg
     3301 ctccggcagg tgactccagc tcgacatcga tgccactgtg gtcgtgggtg aaacctttga
     3361 cgcggtgctg ccacaagacc tggcatccgc cgatctgttg taacgcctgc agcagcaatt
     3421 gctcaagcgc ggcgtggtgc aatgtgacct gttggggata gcctgagcct ggtaggtggg
     3481 cgggcaacgc cgcgctgtag agctgcgagg cgcgcaagaa gacgcgctct acagcgggga
     3541 tcagaccttg cgaggcgaca aactgtccca ggccgttctc ccatgcgtcc agaatgctga
     3601 gcgtggtagc atcgagcagg gcgacctggt tgctttccag cgaaactcct tcgccttcat
     3661 caagcacgac actgggtatt ttatagcgcg ccagccctaa cgcggtgata aggccaacgg
     3721 gattcgcgcc gacgatcagg atggggtcgt gccaatattt tgctgccgga agttgtgcaa
     3781 tatcctgggc taccatcttt tttcattcct gacatgctac gaaataaatt gtctccacct
     3841 gatgttagga aaagat
//
LOCUS       DASZAK010000301         4658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_290842, whole genome shotgun sequence.
ACCESSION   DASZAK010000301 DASZAK010000000
VERSION     DASZAK010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4658
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290842"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1502)
                     /locus_tag="VGT44_05295"
     CDS             complement(129..1502)
                     /locus_tag="VGT44_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEV2580248.1"
                     /translation="MQELQAPLEGGRFFGTLAERRAAGKELRNRVPRSSHAEWAAAAQ
                     RPEPISLLDEQNRTRLAQLVPLRFERMTVSPFTFLRGSPIVMAQDLATTPVTGIHVQI
                     CGDAHLNNFGIYATPERNQVFDVNDFDETLPGPWEWDIKRLAASAMIAGRTNSFPAAI
                     NRRAVQGCVQSYRDHIWQYSNLSNIEVWYSRIDYESSLQFVNRSFRWYIDKQREKSRQ
                     RSSLQVLPKIAVQIDGQYQIKEDPPLITRLADEALKQELREMVDTYRPTIQEDRQVLL
                     SKYHYVDVAQKVVGVGSVGTRCYVVLLLGNDGNDPLVLQIKEAQSSVLERHLGPSVYL
                     NHAHRVVAGQRLMQAASDVFLGWTRAGTRDYYLRQLRDMKLSVDLTTLSPEGFIEYCR
                     FCGWALARAHARSGDSAPISGYLGSSDVFDRAIVTFAETYADQTERDHATFVTELQAR
                     MTAKNQE"
     gene            complement(1745..3133)
                     /locus_tag="VGT44_05300"
     CDS             complement(1745..3133)
                     /locus_tag="VGT44_05300"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2580249.1"
                     /translation="MLKKHKRLLGICILLIILAGCDNPISQPVTITPLALTPRATGTV
                     APVSGTDWTTYHANNARTGYVANTPDPGQLTRAWSVQLDEAVYAEPLVIGARVIVATE
                     GDTLYALDKSTGNIEWHTNVGTPVPLSTLPCGNIDPLGITGTPVYDPATGLVFAVAEI
                     TGPAHILVGIDVATGQVRVRRVVDIPAMDPRANQQRAALALYKNMVYVAYGGLDGDCS
                     DYRGTVVASHTNGSGPLLSYQVPTPREGGIWAASGPAIDSSGNVYVAVGNGSTTQGAW
                     DHSDSVLRLSPTLALEDGFAPDQWAQENAGDVDLGSMGPILLPGGLIFADGKSGQGYL
                     LHADALGGVGGQAQVATICHSFGGAAALDSTVFVPCVEGLREVQVGSGASLMPGWQAG
                     GQITGSPVVGGHTVYSLDSAGGVLYALDSANGTVITTIGVGATSRFASPTISGNQVFV
                     GTMTGVAAVTIS"
     gene            3263..4210
                     /locus_tag="VGT44_05305"
     CDS             3263..4210
                     /locus_tag="VGT44_05305"
                     /inference="COORDINATES: protein motif:HMM:NF020476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HEV2580250.1"
                     /translation="MIISASYKTDIPTFYGEWFMNRLQAGYCKMVNPYSQQVYRVDLT
                     PEEVDGFVFWTKNIGPFLTHLPEVQRRGYPFIVQRTITGYPRELEFRVIDYSHTIEYM
                     RTLADAYGPGVPVWRYDPIIISSLTPPDWHRRNFETLAQGLEGATDEVIISFGQIYKK
                     SRRNMAWAAREFGFDWSLHESLSLPDVRDLVNDLAQVARSHGMRMKVCSQKALIILGM
                     TEEARCVDAERLERVSGRSIVENVALRGNRKECACFASRDIGEYDTCPHGCVYCYAVQ
                     HRNLALQRYKAHDPAGEFLFPPTPNSRTIAMTPRKGSSE"
     gene            4300..>4658
                     /locus_tag="VGT44_05310"
     CDS             4300..>4658
                     /locus_tag="VGT44_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HEV2580251.1"
                     /translation="MTQTIFQVDSFTNVPFAGNPAGVCILREPREDQWMQNVAKEMNL
                     SETAFLLRQDDGYSLRWFTPAVEVDLCGHATLASAQILWETVALAAGQQARFHTRSGL
                     LTADQRGEWIEMDFPAKP"
BASE COUNT          967 a         1376 c         1341 g          974 t
ORIGIN      
        1 attgtcaccc tgagcgaagc gaagggtctc ggtcgagggg cgcagagatg cttcgcttcg
       61 ctcagcatga cattgtcgat gttggccgtg aatcttcatg atcggctgtc ggcacggtaa
      121 accggtgcct actcttgatt ctttgcagtc attctggcct gtaactcagt tacaaaggtg
      181 gcatggtcgc gctccgtctg gtcggcgtag gtctcggcga aggtgacgat cgcccggtca
      241 aacacgtcgc ttgacccaag atagccgctg attggtgcgg agtcgccaga gcgggcgtgg
      301 gcgcgcgcca gcgcccatcc gcagaaccgg caatattcga taaagccctc aggactaagg
      361 gtcgtgagat cgaccgacaa cttcatatcc cggagttggc gcaggtagta atccctggtc
      421 cccgcccgcg tccaccccag aaacacgtcg ctggctgcct gcatcagacg ctggccggcg
      481 acgacgcggt gcgcgtggtt gagatagacg ctcggtccga ggtggcgttc gaggacggag
      541 gactgcgcct ctttaatctg caacacaagt gggtcattgc catcgttgcc cagcagcagg
      601 acaacataac agcgcgttcc gacgcttcct acaccgacga ccttctgagc gacatccaca
      661 tagtggtatt tgctgagcag cacctgccgg tcttcctgga tggtagggcg ataggtatcc
      721 accatctccc gcagttcttg tttgagcgct tcatcggcga ggcgcgtaat caggggaggg
      781 tcctccttga tttgatactg gccatcgatt tgcacggcga ttttgggaag cacttgcaag
      841 ctcgaacgct ggcgagactt ttcacgctgt ttgtcgatat accagcgaaa cgagcgattg
      901 acgaattgta atgagctttc gtagtcgatg cgggagtacc agacttcgat gttgctcaag
      961 ttgctgtatt gccagatatg atcgcgatac gactggacgc agccctggac tgctcgccgg
     1021 ttgatcgctg ctggaaagct attcgtgcgc ccggcgatca tcgcgctggc ggcaagccgc
     1081 ttgatgtccc attcccaggg gccgggtagg gtctcgtcaa agtcattgac atcgaagacc
     1141 tggttgcgtt caggcgtggc gtagatgcca aaattgttga gatgcgcgtc gccacagatc
     1201 tgaacatgaa tgccggtgac gggggtcgtg gccagatcct gcgccatgac gatgggcgaa
     1261 ccacgcagaa aggtaaaagg cgatacggtc atgcgttcaa agcgaagcgg cacaagttgc
     1321 gccaggcgag tgcgattctg ctcgtccaac aggctgatcg gttccggtcg ttgagcagcc
     1381 gctgcccact ccgcatgact ggaacgaggc accctgttgc gcaattcttt gcctgcggcc
     1441 cggcgttctg ccagtgtccc aaagaaccgc cctccttcta aaggcgcctg taattcctgc
     1501 atggccagtc tccttccttg tatgaagttt cacgaccaaa gtcggttctg tcatcctgag
     1561 cgcagcgaag gatatcggtc tccggcgtgt gagatccttc gctgcgctca ggatgacaac
     1621 ttcttaccga tcttgtttgt gaaacttcat tgtcagcctg ggtgggttgt cgacgcgctc
     1681 cacaggccga taaatcggcg gtgcgcacga taaatcggtg cctacgggaa tcactatatc
     1741 cttgtcagga tattgttact gctgctaccc ctgtcatcgt tcccacgaat acctggttgc
     1801 ccgagatcgt tggggaggcg aagcgcgacg tagcccccac ccctatagta gtaatcaccg
     1861 ttccgttggc gctgtcaaga gcatagagta cgccccctgc ggagtccagg ctataaacgg
     1921 tatggcctcc gacaacaggc gaaccggtga tctgccctcc cgcttgccag cctggcatca
     1981 gactagctcc cgacccgacc tgcacctcgc gcagcccctc gacacatggt acaaacaccg
     2041 ttgagtcgag ggcggcagcg ccgccgaacg agtgacagat cgtcgcgacc tgggcctgac
     2101 cgccaacgcc gcccagggcg tcggcgtgca gcagatagcc ctggccggac ttgccatcgg
     2161 cgaagatcag ccctcccggc aagagaatcg gccccatcga gccgagatca acatctccgg
     2221 cgttctcttg tgcccactgg tcgggcgcaa atccatcttc cagggctagt gtcggcgaca
     2281 ggcgcaggac agaatcgctg tggtcccacg ctccctgggt cgtggagccg ttgcctactg
     2341 caacatatac attaccagag gaatcgatgg ccggtccaga tgccgcccag atgccgcctt
     2401 cgcgaggggt tggcacctgg taggaaagca gtggtccgct cccattggtg tgggaagcga
     2461 cgaccgttcc acgataatcg ctgcaatccc cgtcgaggcc gccgtaggcc acgtagacca
     2521 tgtttttgta gagcgccagc gccgctcgct gctggttcgc tcgtgggtcc atcgcaggga
     2581 tatcgaccac gcggcgtacg cgcacttgcc ccgtcgccac gtcgatgccg accaggatat
     2641 gtgcaggccc ggtgatttcc gcgacggcga aaaccagtcc ggttgccgga tcatagaccg
     2701 gagtgccggt aatccccagc ggatcgatgt tgccgcaggg cagcgtggaa agcgggactg
     2761 gagtgccaac gttggtatgc cactctatgt ttcctgtgct tttatcgagc gcgtacagcg
     2821 tatcaccttc cgtcgccaca atcacgcgcg ccccgataac tagcggctcg gcatagacag
     2881 cttcgtcaag ttgaacgctc cacgcgcgcg tgagttggcc tggatcgggc gtattcgcga
     2941 catagccggt gcgcgcgttg ttggcgtgat aggtcgtcca atctgtgcca ctcaccggcg
     3001 caactgttcc cgtcgccctc ggcgtgagcg ccaggggggt gatcgtcacc ggctgagata
     3061 tgggattgtc gcagcccgcg aggataatca gcaaaataca tattcccagg agtcgcttgt
     3121 gtttcttcaa catacttcgg aatctcctcg tgtagtctaa cgcaactttt cgccacatcc
     3181 actcgctaat aatagtacgc tgagtgacta catgctttgt ctgaacatcg accagggtag
     3241 taccatctgc ttagaggatt taatgataat ttctgccagt tacaaaaccg atatacccac
     3301 attttacggt gaatggttta tgaaccgttt gcaggccggt tattgcaaga tggtcaatcc
     3361 ctacagccag caggtgtatc gcgttgatct gacccctgaa gaggtggatg gcttcgtctt
     3421 ctggacaaaa aacatcggac cattccttac acacctgcca gaagtgcagc gcagaggcta
     3481 tccctttatc gtgcagcgta ccatcaccgg ctacccgcgc gaactggaat tccgcgtcat
     3541 cgactacagt cataccatcg aatatatgcg aaccctggcc gacgcctacg gtcctggggt
     3601 acccgtctgg cgctacgacc cgatcatcat atcatccctg accccgccgg actggcaccg
     3661 gcgcaacttt gagacgctgg ctcagggact ggaaggcgcg accgatgagg ttatcatctc
     3721 gtttgggcaa atctacaaga agagcaggcg caatatggcc tgggccgcgc gcgagttcgg
     3781 ctttgattgg agcttgcacg aaagtctctc gctgccagac gtgcgcgatc tggtcaacga
     3841 cctggcgcag gtggcccgtt cgcacgggat gcgcatgaag gtctgctcgc agaaagcatt
     3901 gattatcctg ggcatgaccg aagaggcgcg ctgtgtcgac gcggaacggc tcgaacgagt
     3961 ctcaggccgc tccattgtcg agaatgttgc gctgcggggc aatcgcaaag aatgcgcctg
     4021 cttcgcctcg cgcgacatcg gcgaatacga cacctgcccg cacggctgcg tctactgcta
     4081 cgccgtgcag catcgcaacc tcgccctgca acgctacaag gcgcacgatc cagcaggcga
     4141 gtttttgttc ccacccacac caaacagcag aacaatagcg atgacgccgc gcaaaggttc
     4201 ctctgaatag ccccgattgg ctggtgcctt aaaccggtgc aggtgataaa gttgagtaga
     4261 ggcgtgatac aaggcacaat cacatcaagg aaagagccaa tgacccagac catatttcaa
     4321 gtagattcct tcaccaatgt cccgttcgcg ggcaatcccg ccggtgtgtg cattctccgc
     4381 gagccgcgcg aagatcaatg gatgcaaaac gtggcgaaag agatgaacct ctctgaaaca
     4441 gccttcctgc tcaggcagga cgacggatat agcctgcgct ggttcacacc cgccgtcgag
     4501 gtcgatttgt gcggccacgc cacgctcgcc agcgcgcaga tcctgtggga aaccgtcgcg
     4561 ctcgcggcag gccaacaggc gcgcttccat acacgcagcg gcctgctcac cgcagatcag
     4621 cgtggcgagt ggatcgagat ggatttcccg gccaaacc
//
LOCUS       DASZAK010000302         1928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_149728, whole genome shotgun sequence.
ACCESSION   DASZAK010000302 DASZAK010000000
VERSION     DASZAK010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149728"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VGT44_05315"
     CDS             <1..245
                     /locus_tag="VGT44_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2580252.1"
                     /translation="LDESTLSFLGLGVQPPTPEWGSMISEAQVYLTSDPWMLFGPTVA
                     IILIVLSLNILGDALRDHLDPRSSGSMGRLGAPVEK"
     gene            334..1446
                     /gene="gcvT"
                     /locus_tag="VGT44_05320"
     CDS             334..1446
                     /gene="gcvT"
                     /locus_tag="VGT44_05320"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEV2580253.1"
                     /translation="MTSQDTTALKRTPLHAEHCALGARMVEFGGWDMPVQYSGILEEH
                     RAVRGRAGLFDVSHMGEFRAGGPGALAFLQHLVPNDVSRLAINQALYTQLCLPDAGTI
                     DDLIIYHLGENHYMLVVNAANIDKDFAWVTGQSAGFDVALTNISDETALVALQGPEAL
                     AILQPLTAVDLGGIVYYHCAPGKVDGIDCIISRTGYTGEDGFELYCAQGDVVALWRGL
                     LAAGKERGLLPAGLGARDTLRLEAGYCLYGHELDDTTNPLEAGLGWTIKLNKGDFIGH
                     DALAAIKAQGLKRKLVGIEMVERGVCRGGYALYDNGRQVGALTSGAPSPTLSKNIGMG
                     YVEIADAVPGKAVQVDIRGKHTAAQVVALPFYKRTR"
     gene            1466..1861
                     /gene="gcvH"
                     /locus_tag="VGT44_05325"
     CDS             1466..1861
                     /gene="gcvH"
                     /locus_tag="VGT44_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEV2580254.1"
                     /translation="MASLNHPANLKYSKTDEWVLVEGDQATVGITDYAQDQLGDIVYI
                     ELPWDAGQTVAHETKFGDIESVKATSELISPLSGEVVKTNEALKDQPELINDNPYQAG
                     WMMVVKMSNPAEVDGLMSADQYKAYLEGR"
BASE COUNT          409 a          552 c          596 g          371 t
ORIGIN      
        1 tcctcgacga atcgacactc agcttcctgg gccttggtgt gcagccgccc acccccgaat
       61 gggggtcgat gatcagcgag gcgcaggtct atcttaccag cgatccctgg atgctcttcg
      121 ggccaaccgt tgccatcatc ctgattgtcc tgagcctgaa tatcctgggc gacgcactgc
      181 gcgatcacct cgatccccgc tcaagtggta gcatgggccg cctgggcgct ccagtggaga
      241 agtgaggaaa gagccatagc aaacctcatg tgtcctgtga tattatgaag ccatgcagac
      301 attcctcaaa aagattgcaa tggagcaacg agtatgacat cacaggatac aacagcgctg
      361 aaacgaaccc ccctccacgc ggaacattgc gcgttgggcg cgcgcatggt cgagtttggc
      421 gggtgggata tgcctgtcca gtatagcggc atcctcgaag aacaccgcgc cgtgcgtggg
      481 cgcgccggtc tcttcgatgt tagtcacatg ggtgaattcc gcgccggggg tcctggcgcg
      541 ctggcctttt tgcaacacct cgtccccaac gatgtcagcc gcctggcgat caaccaggcg
      601 ctgtacacgc agctttgcct gccggacgcg ggaaccatcg atgacctgat tatttatcac
      661 ctgggcgaaa accattatat gctggtggtc aatgccgcca acatcgacaa agattttgcc
      721 tgggtcacag ggcagagcgc tggcttcgat gtcgcgctga ccaatatttc cgacgagacc
      781 gcgctggtcg ccttgcaagg gccggaggcg ctggccatct tacagccact cacggcggta
      841 gacctgggcg gcatcgtcta ttaccactgc gcgccgggga aggtagatgg catcgattgc
      901 atcatctcgc gcacgggcta tacgggcgaa gacggctttg aactctactg cgcgcagggg
      961 gatgtcgtgg cgctgtggcg cgggctgctg gccgccggaa aggagcgcgg gctgctgcct
     1021 gccgggctgg gcgcgcgtga cacgctgcgg ctggaggctg gctactgcct gtatgggcat
     1081 gaactggatg ataccaccaa cccgctcgag gccggcctgg gctggacgat caagctcaac
     1141 aagggcgact ttatcgggca tgacgcgctg gccgcgatca aggcgcaggg gctgaagcgc
     1201 aagctggtcg gcatcgagat ggtcgagcgc ggcgtctgtc gcggcggcta tgctctctac
     1261 gacaatgggc ggcaggttgg agcattgacg agcggcgcgc ctagcccgac tctcagcaaa
     1321 aacatcggta tgggttatgt cgagatcgcc gacgctgtgc cgggcaaggc cgtgcaggtc
     1381 gatatccgag gaaaacatac ggcggcgcag gttgttgcgc tgccatttta taaacgaacg
     1441 cgataaagaa gggagactcg tatttatggc aagcctcaat catcccgcga acctcaaata
     1501 tagcaagacc gatgagtggg tgctggtcga gggcgaccag gccaccgtcg gcatcactga
     1561 ctacgcccag gatcaactgg gcgacattgt ctacattgaa ttgccctggg acgccgggca
     1621 gacggtcgcc cacgaaacga aattcggcga tatcgagtcc gtcaaggcca cctctgagct
     1681 tatttcgccc ctcagcggcg aggtcgttaa gaccaacgag gcgttgaagg atcagcccga
     1741 gctgattaac gacaacccct accaggctgg ctggatgatg gtcgtcaaga tgtccaaccc
     1801 cgccgaggtt gacggcctga tgagcgccga tcagtataag gcatatttag agggtaggta
     1861 atcgtacaag ccaatccttc tcaatgcgtg ttgcatggcg accgcaaggg tcgccactac
     1921 aataacgc
//
LOCUS       DASZAK010000303         2252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_434159, whole genome shotgun sequence.
ACCESSION   DASZAK010000303 DASZAK010000000
VERSION     DASZAK010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434159"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1275
                     /locus_tag="VGT44_05330"
     CDS             <1..1275
                     /locus_tag="VGT44_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEV2580255.1"
                     /translation="LNDAGLRVWRNREGSNLMRGIAGALVIRAEMSGNLRRKGQAVSI
                     FEVDEAVVPQIMHDVPLRVAVFNNLFRDQLDRYGEVDSVSVRWQQAIGTLPELTVLAL
                     NADDPAVAYLGTQYAGPVVYFGIDDTALDLAAQGISSERHQTIDTRTCPRCGSDFAYT
                     TRFYSHLGHYTCHTCGNTRPEPQVRAINITMNAFDQLSMTVVTPTHSGEIVIPLPGFY
                     NVYNALAAIATTQALQIDWTPVVTGIQQFKPAFGRGERVQVAGRTLRILLAKNPTGFN
                     EVLRTVYSGDEARYVLMMLNDNIADGRDVSWIWDVDFERIIDHTAALTVTGTRALDLA
                     LRLKYAGVAPEAIDIIPTIPLRAAARKSAAPEKPLIRQRVGLARALDAAIERTPVGET
                     LFLIPTYTGLLDIHRELERRGLTPHYWEEKGP"
     gene            1272..2069
                     /locus_tag="VGT44_05335"
     CDS             1272..2069
                     /locus_tag="VGT44_05335"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580256.1"
                     /translation="MSDHGQPAYTLTLGHLYPDQLNLYGDRGNILTLQRRCRMRGINL
                     RVVGLGIGDALAPDEYDLLFIGGGQDKEQAPVAQDLLDMKGIGLWAAVQDDMPVLAVC
                     GGYQLLAHYYRPASGPDMRGLGVFDAWTIHKGVHVPRCIGNIAISWNGTTLVGFENHG
                     GRTYLGKVKPLGKVLRGHGNNNEDRTEGAVYRNAYGTYLHGSLLTKNPHFADHLITLA
                     LKRTYGLNEQDLKEVFPLDDALEWEAHAFMLERLGLHPAAGAALRYP"
BASE COUNT          461 a          685 c          667 g          439 t
ORIGIN      
        1 ctcaacgacg ccgggctgcg cgtctggcgc aaccgtgaag gctccaactt gatgcgcggc
       61 atcgccgggg cgctggtgat tcgcgcggag atgagcggca acctgcggcg taaaggtcag
      121 gccgtctcga tctttgaggt agacgaagcg gtagtaccac agatcatgca cgatgttccg
      181 ctgcgcgtcg ccgtcttcaa taacctcttt cgcgaccagc ttgaccgcta tggcgaggtt
      241 gacagcgtct cggtgcgctg gcagcaggct atcggcaccc tgccggagtt gaccgtcctg
      301 gcattgaacg ccgacgatcc cgccgtagct tatctcggca cgcaatacgc cggcccggtc
      361 gtctacttcg gcatcgacga taccgcgctc gacctggccg cgcagggtat ttcatccgaa
      421 cgacaccaga ccatcgatac acgcacctgc ccacgctgtg gcagcgactt tgcgtatacc
      481 acgcgctttt acagccacct cggccactat acctgccaca cctgcggcaa cacacggcca
      541 gaaccgcagg tgcgcgccat caacatcacc atgaacgcct ttgaccagct ctccatgacg
      601 gtcgtcacgc ccacgcacag tggtgagatc gtcattcctt tgccgggttt ttacaatgtc
      661 tacaatgccc tggccgccat cgccacaacc caggcgctgc aaatcgactg gacgccggta
      721 gttacgggca tccagcagtt caaaccagct ttcgggcgtg gagagcgcgt gcaggtcgcc
      781 gggcgcacgc tgcgcatctt gctggccaaa aaccctaccg gctttaatga ggtgctgcgc
      841 actgtgtata gtggcgacga agcgcgctac gtcctgatga tgctgaacga caacatagca
      901 gatggacgcg atgtctcctg gatctgggac gttgatttcg agcgtatcat cgatcacacc
      961 gccgctctga cggtcacggg gacacgcgcg ctcgatctcg cgcttcgtct caaatatgcg
     1021 ggggtcgcgc cggaggctat cgacatcatc cctaccatcc cattgcgagc ggcggcaagg
     1081 aaatcagccg cgccggagaa gccattgatt cgccagcgcg tgggcctggc gcgtgcgctt
     1141 gatgccgcca tcgagcgcac accggttggt gagacattat tcctcattcc aacgtataca
     1201 ggattgctgg atattcatcg cgaactggaa cggcgcgggt taacgccgca ctactgggag
     1261 gagaaaggcc catgagcgac cacggccagc ccgcctacac gctgacgctg gggcatctct
     1321 accccgacca gttgaacctg tacggagatc gcggcaacat cctgacgctg cagcgccgtt
     1381 gccggatgcg cggtatcaac ctgcgcgtcg tcggcctggg tatcggggac gcgctggcgc
     1441 ccgacgaata cgatctgctc ttcatcggcg gcggccagga caaagagcaa gctccggtag
     1501 cccaggactt gctcgatatg aagggtatcg gcctttgggc tgctgtgcag gacgatatgc
     1561 cggtgctggc cgtctgcggc gggtatcaat tgctggcgca ctattaccgg ccggccagcg
     1621 ggccggatat gcgcggcctg ggcgtcttcg acgcctggac gatccataag ggagtgcatg
     1681 taccgcgctg cattggcaac atcgccatca gttggaacgg cacaaccctg gtcggctttg
     1741 aaaatcacgg cggacgcacc tatctcggta aggtcaagcc gctcggcaag gtgctgcggg
     1801 ggcatggcaa caacaacgaa gatcgcacag agggcgcggt ctatcgcaac gcctatggca
     1861 cctacctgca tggctcgctc ttaacgaaaa atccgcattt cgccgatcac ctgattacct
     1921 tagcattgaa acgcacgtat ggattaaatg agcaggattt gaaggaagtc tttccgctcg
     1981 atgatgccct cgagtgggag gcgcacgctt ttatgctgga acgtttagga ttacacccgg
     2041 cggcaggagc cgcgttacgc tatccatgaa gaccaatgac ggcctgcatg gatgaccaga
     2101 ccaggaaggg acctatgagc tttgatcgat cactagctcg atgctcattc tgtggacgac
     2161 gcccgccaga ggtacgacgt atggttgccg ggccgggcgc ggcctacatt tgtgacgaat
     2221 gcctgaactt atgccaggag attttagccg at
//
LOCUS       DASZAK010000304         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_469124, whole genome shotgun sequence.
ACCESSION   DASZAK010000304 DASZAK010000000
VERSION     DASZAK010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469124"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VGT44_05340"
     CDS             <1..675
                     /locus_tag="VGT44_05340"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580257.1"
                     /translation="MLPDFKARLEWFLEHRPQLASLVSRWHEPGMGERRLLALVRGHR
                     MKRLLKRMLDPEEFLGDYGIRGISKYHAEHPYVYRVHETEYTVAYDAGESSSGVFGGN
                     SNWRGPIWFPINFLLIETLQKFHHYYGDDFLVECPTGSGKKLTLWQIADQLSCRLTHI
                     FLRGQDGRRPVFGTNELFQTDPHWRDYPLFYEYFHGDTGRGVGASHQTGWTGSVAKLL
                     EQTEGR"
     gene            699..>1631
                     /locus_tag="VGT44_05345"
     CDS             699..>1631
                     /locus_tag="VGT44_05345"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HEV2580258.1"
                     /translation="MAKSILISPATAIDHPLVHFGREVCGDLATGLRREWLVTNGLGG
                     YASGTLAGVNTRRYHGLLVAALTPPVERTVLVGGLIEWATYNGQRYPLSTHEYGDGTI
                     DPHGYRHLQSFRLQGGLPVWTFALGNALLERRVWMAYGANTTYVMYRLLRGTDELTLE
                     ITPLVTYRDFHSLNSGQGWRPGVEALSNGAVIRAWDGARPFQLLADNGRFAPGGDWYW
                     NFRHREEAARGLDDRSDLYAPGNFTVTLLPGASYALVLTTETQVEMNSSIALANEEER
                     QLRLLRRANAESAHPVVQQLTLAADQFIVQRWFSS"
BASE COUNT          308 a          458 c          504 g          361 t
ORIGIN      
        1 atgctgcccg atttcaaggc tcgcctggaa tggttcctgg agcaccggcc acaactggcg
       61 agcctggtgt cacgctggca cgagccaggg atgggcgagc gccgcctgct ggcactggtg
      121 cgtggtcatc gcatgaagcg cctgctcaag cgcatgctcg acccagagga gtttttgggc
      181 gattacggca tccgtggcat cagcaagtat catgctgaac acccctacgt ctatcgtgtg
      241 catgagacgg agtacacggt cgcgtatgat gcgggtgagt caagcagcgg cgtcttcggt
      301 ggaaattcca actggcgggg gccgatctgg ttcccgatca acttcctgct cattgagacc
      361 ctccagaagt tccaccacta ttatggtgac gatttcctgg tggagtgccc taccgggtct
      421 ggtaagaagc tgacactctg gcagattgca gatcagctct cgtgccggct tacgcacatc
      481 tttctgcgtg ggcaggacgg gcgccgccct gtcttcggca cgaatgagct cttccagact
      541 gacccgcatt ggcgcgacta cccgcttttc tacgagtact tccacggaga tacgggcagg
      601 ggcgtcggtg ccagtcatca gacgggttgg acgggatcgg ttgcgaagtt gcttgagcag
      661 actgaaggta ggtaagtcac gagaggagca ggtgtaccat ggcgaagtct atattgatat
      721 cgccagccac agcaatcgat caccctctgg ttcacttcgg gcgcgaggtc tgtggcgacc
      781 tcgcgacagg gctacggcgg gaatggctcg tgacgaacgg gcttggtggc tacgcctcgg
      841 gtacacttgc aggagtgaac actcggcgct atcatgggct actggttgcg gcgctcacgc
      901 ctcccgttga gcgtaccgtg cttgtgggtg gccttataga gtgggccact tacaacggtc
      961 agcgctaccc gctctcgacg catgagtacg gggatgggac gatagaccca catggctacc
     1021 gtcatctcca gtcgttcaga ttacagggtg gactgcctgt atggactttc gccctgggta
     1081 atgcactgtt ggagcggcgt gtgtggatgg cctatggtgc caacaccact tacgtgatgt
     1141 atcgcctgct gcggggcacc gacgaactca cgttggaaat cacaccgctc gtcacgtacc
     1201 gtgattttca ctcgctgaac tccggtcaag gctggcgacc aggagtagag gctttgtcaa
     1261 atggggctgt gatccgcgcg tgggatggtg cgcggccatt tcagttgctc gcagataacg
     1321 gtcgcttcgc tcccggcggt gactggtatt ggaactttcg tcatcgcgag gaggcagcac
     1381 gggggctgga tgaccgctcc gacctctacg ctcctggcaa cttcaccgtt accctgctcc
     1441 caggtgccag ttatgcgctt gttctgacga ccgagacgca ggtggaaatg aacagcagta
     1501 ttgccctcgc caatgaggaa gagcggcagt tgcgattgct gcgtcgtgct aacgccgaat
     1561 ctgcccatcc agtagtccag caactcacac ttgccgctga ccagttcatc gtccagcgtt
     1621 ggttctcgtc t
//
LOCUS       DASZAK010000305         6449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_256688, whole genome shotgun sequence.
ACCESSION   DASZAK010000305 DASZAK010000000
VERSION     DASZAK010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256688"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..752)
                     /locus_tag="VGT44_05350"
     CDS             complement(78..752)
                     /locus_tag="VGT44_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580259.1"
                     /translation="MLLICLAYQGWFAPAPAHAQAAQGDAYIVTILGNTQPPGYSPAL
                     LTVHVYDTVVFVNRSVPAASYAVIANDGSFSSPSIQPGQQWSVTFSSPGAFEYQESAS
                     SPRMTGVIVVAPASVPLLPAPLPAAQATAIAYVQAGKMPPDNISQQAVAPAQQTSPGQ
                     QGKSIISPFASPGLFALFFAGAAVCIVALELLGFGIMKWSKPTYQKIKTRIREMRLSW
                     KDDDDE"
     gene            complement(960..1775)
                     /locus_tag="VGT44_05355"
     CDS             complement(960..1775)
                     /locus_tag="VGT44_05355"
                     /inference="COORDINATES: protein motif:HMM:NF025864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HEV2580260.1"
                     /translation="MNLLELEGLIRQCLDEFYMRRIKKLSELRLREVLSKKNPYLLRA
                     TGIRKASEIVAEVLRAYMSSSDESIFGDAFFEPIARICSGGTVSPSEGVDVAVESDTV
                     YKAFSIKSGPNIFNASQAKRQDDEFNSLRHRMYKVHKQFDPILGHCYGRRSSGRKSKR
                     SYRILSGQALWEELTGDVDFYIKLIDLMRDHPTEHRRTFEIEWDNAINRFEGEFIINF
                     GNLDGSINWEKLLRYNSGKEQIPWVKVATVVSVDDEDEGIEDEGMDEEAVDEF"
     gene            complement(1756..2727)
                     /locus_tag="VGT44_05360"
     CDS             complement(1756..2727)
                     /locus_tag="VGT44_05360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV2580261.1"
                     /translation="MSEDQDWQTRLFEHDCLDILRWLPSECVDLIVTSPPYADQRKDT
                     YGGVHPDRYVEWFLPIAAELKRVLKKEGSFILNIKERVMNGERHTYVLELILALRKQG
                     WLWTEEYMWHKRNSYPGKWPNRFRDGWERCLHFTKDRHFAMYQDAVRVPMGDWSQARL
                     RNLSQTDRRRDNSRVQSGFGKKIENWVGRELAYPDNVLHLATECSNQGHSATFPVELP
                     SWFIKLFTQPGDVILDPFVGSGTTALAARQLGRRCIGIDINHEYVQLAQERLDTLSPQ
                     FSVSLFGEEYSNKGIPEQHLNAKPSPAPLFTEENDVEGNEYYESVGT"
     gene            complement(2868..3494)
                     /locus_tag="VGT44_05365"
     CDS             complement(2868..3494)
                     /locus_tag="VGT44_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2580262.1"
                     /translation="MHVNIPTPTGYLEGILKPEEDGVEPLFGAVVCHPHPLGGGTMHN
                     KVVFKVAQTLQTLGMPTLRFNFRGVGHSAGSYDEGRGEMDDARYALEFLSRRYPVLPV
                     IMAGFSFGAYVGLRVAAVDDRAQAMIGLGTPARMFGDDHLQGSHKPKLLVHGTLDDLA
                     PYDLTARWFEQVPAPKRLVVIQGADHFFQDRLDEVQGIVASFVRDLMR"
     gene            complement(3526..4905)
                     /locus_tag="VGT44_05370"
     CDS             complement(3526..4905)
                     /locus_tag="VGT44_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2580263.1"
                     /translation="MPESIQPAQSFAQTRGAELEIRRYQAADYDDVWRLHELALQEVG
                     AFLGHGYWDDDLHHIEEVYLQNRGEFFVGLLDGRIVAMGALRKTSLERAEIKRMRTHP
                     SVQGRGYGQLILDELERRAAALGYTTLHLDTSVVQLVAQKFYTKNGYKETEREMRRWL
                     ECILFEKQIESTHTPPKEQPMPESTQPARGFAELNGAKLYYEIAGAGHPLVLLHEGIA
                     DSRMYDDQFYNFAQQYRVVRYDLPSFGQSDIPPVNEPISLSENLRGLLAFLGIEKTYL
                     LGMSMGGSIALDFTLAHPDMVDALILAASGLSGHEFTSFETVYKAIGEEIEEAAKRGD
                     YERASELETRIWVDGPERTPEQVDPLLRRRMYEMNLYNYQRLGNAEFPPPLPLDPPAI
                     ARLNEVAVPTCLIIGDKDVRELLGVMDILEQGIPGAKKVVMHGVAHALNLEQPEEFNR
                     IVLDFLRSL"
     gene            5139..6356
                     /locus_tag="VGT44_05375"
     CDS             5139..6356
                     /locus_tag="VGT44_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEV2580264.1"
                     /translation="MKRHILIQYIGLGGALLLLAFQCFAHVTLTYSAQVPRPMVPSRA
                     IGAPVFSRPLHLAPQLHVWRNVLEDNMNQPVRLVGANRSGSEYACLGWSIFDGPSDQA
                     SINAMLSWRINAIRIPLNEDCWLNINMHGSPYGGAVYQQAIERYVQLLIGDDITPILD
                     LHWTAPGSQQATSQQAMPDRDHSLTFWWQVAEAFKWNNAIIFDLFNEPFPDSDRDTLT
                     AWNCWRDGTNANTCPFGTGGLTYNTAGMQELVNTVRGTGARNVIILGGIQYASTLDHW
                     MTFAPNDPAHELAVSWHLYNFSHCNQLSCWSAEGLPVMRVYPVVTGEIGENSTGGNFV
                     LSLMDFLDHPASNLPPQNYLAWAWNTDQTVFDLITSYSGAPTIPYGVEYQQHLLGEAK
                     QAADRRRPPAGHA"
BASE COUNT         1442 a         1824 c         1711 g         1472 t
ORIGIN      
        1 tgtgctcagg atgacaggat tggctttggt cgggaaaatt catcatcgca ttcctactac
       61 gtggtgaggg atgtgcttca ttcgtcatcg tcatccttcc agctcaggcg catttcgcgg
      121 attctggttt tgatcttctg ataggtgggt ttgctccatt tcattatccc gaatccgagc
      181 aattccagcg cgacgatgca cacagcggct cccgcgaaga aaagcgcgaa gagtcctggc
      241 gaggcaaacg ggctgatgat cgatttcccc tgctggcctg gcgaggtttg ctgcgcgggc
      301 gcaacggctt gctgcgagat gttatccggc ggcatcttgc ccgcctggac ataggcgatg
      361 gcggtggcct gcgctgccgg tagcggcgct gggagcagcg gaactgacgc tggcgccacg
      421 acgatcaccc cggtcatacg cggtgacgag gcgctttcct ggtactcgaa tgcgccagga
      481 ctgctgaacg tcacgctcca ctgctgcccc ggctggatag acggcgaaga aaagctgcca
      541 tcattcgcaa tgacggcata agacgcagcc ggtaccgacc ggttgacgaa cacgacggta
      601 tcatagacat gcaccgtcaa cagcgcgggc gagtagcccg gcggctgcgt gttgcctaaa
      661 atcgtcacga tgtaggcatc gccctgtgcc gcctgcgcat gtgcaggggc gggcgcgaac
      721 cagccctgat aggccaggca gatcagtagc acgctgcata ggaatagagg atatacgctt
      781 aaccgttgcg attggtactg ttgtgactcg tgacttttca tttcaaagct atcccgatag
      841 cgtagtattg ctacattcag gatagcgaaa cattacaaac aggaatactg aatctatctt
      901 cttggttcat gaaaggcgaa aacatgtgca tgtgagacaa gaaagtatag aagagccgct
      961 caaaactcat ctacggcttc ctcgtccatg ccctcatctt caatgccctc atcctcatcg
     1021 tctacactaa ctacggtcgc aacttttacc catgggatct gctcttttcc actgttgtaa
     1081 cgtagcaact tttcccaatt gatactgcca tcgaggttgc cgaagttaat aataaattct
     1141 ccctcaaacc tgttaatggc attatcccac tcaatttcaa atgtacgacg atgttctgtt
     1201 gggtggtcac gcattaaatc aattagcttg atataaaaat ccacatctcc cgtcaattct
     1261 tcccaaagag cttgtcccga aagaatacgg taactgcgtt tgcttttccg acctgaagat
     1321 cttcggccat agcaatgtcc cagaatcgga tcaaattgct tatgcacttt atacatgcga
     1381 tgccgcaggc tattaaattc atcatcctgg cgtttagcct gactcgcatt gaaaatattt
     1441 ggaccagact ttatagagaa cgctttgtaa accgtatctg actcaacagc gacatcaacg
     1501 ccttcactcg gtgaaacagt tcctccactg catattcttg ctatcggctc aaaaaacgca
     1561 tcaccaaaga tactctcatc agatgatgac atataagcgc gaagcacttc agctacaatt
     1621 tcggatgcct ttctgatacc ggtcgctcgg aggaggtaag ggttcttctt actgagaacc
     1681 tctcttaatc gaagctccga cagtttcttt atccgccgca tataaaactc atcaaggcat
     1741 tgacgtatta agccctcaag ttccaacaga ttcataatat tcgttacctt ctacatcgtt
     1801 ctcttcagtg aataatggag caggagacgg cttggcatta agatgttgct caggtattcc
     1861 cttattgcta tattcctccc cgaacagaga aactgagaat tgtggggaaa gagtatccag
     1921 gcgctcttga gcgagttgca cgtattcatg gttgatatcg ataccgatac agcgcctacc
     1981 taattgcctg gcagctaatg ccgttgtgcc agaacccaca aatggatcga ggatcacatc
     2041 tccgggctgc gtaaagagct taataaacca ggaaggcaac tcaacgggaa aggtggcgct
     2101 atgcccttga ttgctacatt ctgttgccag atgcaggacg ttatctggat aggccagttc
     2161 tcggcccacc caattttcaa ttttcttgcc aaagccactt tgtacgcggg aattgtcacg
     2221 ccgccgatct gtctgactaa ggttacgcag acgggcctga ctccaatcgc ccatcggtac
     2281 gcggacagca tcctggtaca tagcgaagtg cctgtcttta gtgaaatgca aacagcgctc
     2341 ccagccgtcg cggaagcggt tcggccattt gccaggatag ctgttgcgtt tgtgccacat
     2401 atactcttcg gtccacagcc atccctgttt gcgcagagcg aggatcagtt caagcacata
     2461 ggtatgccgc tcgccattca taacgcgctc tttaatgtta aggataaaag agccttcttt
     2521 cttcaagaca cgcttgagtt cagccgcgat ggggagaaac cattcgacat acctgtcggg
     2581 atgaacgcct ccataggtgt ctttgcgctg atccgcatag gggggagaag ttacaatcag
     2641 atcaacgcat tcggaaggta gccagcgcaa aatatccagg caatcatgct caaacaaacg
     2701 agtttgccag tcctgatctt cactcatacc aatataccag tcctctaacc taaacggagc
     2761 ctaccaaatt tcccctcatt ataccatgct tgcacacccg ttcggtattg gtgtggcatt
     2821 attttctcat ttcatgaaag gcaagagcca ggagtgtaag agatggttca cctcatcaaa
     2881 tcccgcacga acgacgccac gatcccctgc acttcgtcca ggcggtcctg gaagaagtgg
     2941 tccgcgccct ggatgacgac caggcgtttt ggggcgggaa cctgctcgaa ccaccgggcg
     3001 gtcaggtcgt agggtgccag atcgtcgagt gtgccgtgta cgagtagctt gggcttgtga
     3061 ctgccttgca ggtggtcgtc gccgaacatg cgcgccgggg ttcccaggcc gatcatggcc
     3121 tgcgcgcgat catcgacggc ggcgacgcgc aggccgacat acgcgccgaa cgagaagccc
     3181 gccataatca cgggcaggac cggatagcgg cggctcaaaa attccagggc gtagcgcgcg
     3241 tcgtccatct cgccgcgtcc ctcgtcgtag ctgcctgcgc tgtgacccac gccgcgaaag
     3301 ttgaaacgca gcgtgggcat ccccaacgtt tgcagcgtct gggccacctt gaacacgacc
     3361 ttgttgtgca ttgtcccacc gcccagggga tgcggatggc agacgaccgc gccgaaaagc
     3421 ggctcaacgc cgtcctcttc gggcttgaga atgccttcta aatatcccgt tggagtcggg
     3481 atgttgacgt gcaatatctg gccgatggga cacccacctt tccttttaca acgaccgcag
     3541 aaaatccagc acgatgcgat tgaactcctc cggctgctct agatttagtg cgtgtgccac
     3601 gccatgcatg acgaccttct tcgcgccagg gataccctgt tcgagtatat ccatgactcc
     3661 cagtaactcg cgcacatcct tgtcgccgat gatgaggcag gtgggaacgg cgacctcgtt
     3721 caggcgagcg atagcagggg ggtccagcgg cagcggtggc ggaaactccg cgtttcctag
     3781 acgctggtag ttgtacaggt tcatctcata catgcgccgt cgaagcagtg ggtcaacctg
     3841 ctctggtgtg cgttcagggc catcaaccca tattctggtc tctaattcgc ttgcccgttc
     3901 gtaatcgccg cgtttagcgg cctcttcgat ttcctccccg atggctttgt ataccgtttc
     3961 gaatgaagtg aattcgtggc cgctcagccc ggatgcggcg aggatcagcg catccaccat
     4021 gtcaggatgt gccaatgtga aatcgagcgc gatggagccg cccatcgaca tacccagcag
     4081 atacgttttc tcgattccca ggaaggcgag cagtccacgc aggttttcac tcaaggagat
     4141 tggctcattc accggaggga tgtccgattg cccaaaactg ggtaggtcgt atcgcacgac
     4201 gcggtactgt tgcgcgaagt tatagaactg gtcatcatac atgcggctat ccgcaattcc
     4261 ttcgtgaagg agtacgagcg gatgccccgc gcccgcgatt tcgtaataga gcttcgcgcc
     4321 gttcagttca gcgaaaccgc gtgccggttg tgttgattct ggcattggtt gctcctttgg
     4381 cggtgtgtgt gtactctcaa tctgcttttc gaagaggatg cattccagcc acctgcgcat
     4441 ctcccgctct gtttctttgt agccgttctt tgtatagaac ttttgcgcga ccagttgcac
     4501 gaccgaggta tccaggtgca gtgtcgtgta accaagcgcg gcggctcgac gctctagctc
     4561 gtcaaggatc aactgcccat aaccgcgccc ctggacagat ggatgcgtcc gcatgcgttt
     4621 gatttcggcg cgttcgagcg aggtcttgcg caaagcgccc attgcgacaa tgcgcccatc
     4681 gagcagacca acgaagaact ccccccggtt ttgcaggtag acctcctcga tatggtgtag
     4741 atcgtcgtcc cagtagccgt gaccgagaaa tgcccccacc tcttgcagcg ctagctcatg
     4801 cagacgccac acgtcgtcat aatcggcggc ctggtagcgc cggatttcca actccgcacc
     4861 cctcgtctgg gcgaagctct gcgccggttg tattgattct ggcatcggtc atttctcatt
     4921 ctacgctcgt aagcggtata cgctttcagc tttcgattat acaccaaagg gaggcagaac
     4981 cgccgcagct agaggaagca aatcaagccc atgaaaaagc ccgtcaactt gggtggaaat
     5041 tctgaaagag agttccctca attagtctcc gcatgtacaa tagataatgg atgatgatga
     5101 atctattctc agggatgttg tagaggagga acaggggcat gaagcgccac attcttattc
     5161 agtatatcgg tttgggaggg gcgctgctgc tgctggcctt ccaatgcttc gcacatgtta
     5221 cgctcacgta ttccgcgcaa gtgccgcgac cgatggttcc gtcgcgagca atcggcgcgc
     5281 cggtgttttc caggccgctg catctcgcgc cgcagctgca cgtatggcga aatgtgctgg
     5341 aagataatat gaaccagccg gtgcgcctgg tcggggcgaa ccgctccggc tctgaatatg
     5401 cctgcctggg ttggagtatc ttcgatggac catccgacca ggcgagcatc aacgcgatgc
     5461 tgagctggcg catcaacgcc atccgcattc cgctcaacga ggactgctgg ctgaacatca
     5521 atatgcatgg cagtccctat ggcggcgcgg tctatcaaca ggccatcgag cgctacgtgc
     5581 agttgctgat cggcgatgac atcacgccca tcctggacct gcactggacg gcccccggca
     5641 gtcagcaagc gaccagccag caggccatgc ccgaccgcga ccactctctg accttctggt
     5701 ggcaggttgc agaggcattc aagtggaaca atgctattat ttttgacctg tttaacgagc
     5761 catttcccga tagcgatcgt gatacgctca cggcttggaa ctgctggcgc gacggcacca
     5821 acgccaatac ctgtcctttt ggcaccggag gattgacgta taataccgcc gggatgcagg
     5881 aactcgtcaa caccgtacgc ggcaccggcg cgcgcaacgt gataatactt ggcggcatcc
     5941 agtacgcctc cacgctggat cactggatga ccttcgcccc caacgacccg gcacacgaac
     6001 tggccgtctc gtggcacctg tacaatttca gccactgcaa ccagctctcg tgctggagcg
     6061 cagaggggct gccggtcatg cgcgtgtacc ccgtcgtcac cggggaaatc ggcgaaaaca
     6121 gcacaggtgg aaatttcgtg ctgagcctga tggatttcct cgatcacccg gccagcaatc
     6181 tgccgccgca gaactacctc gcctgggcct ggaacaccga ccagaccgtc ttcgacctga
     6241 ttaccagcta cagcggcgcg ccaacaatcc cctacggcgt tgaataccag caacatttgc
     6301 tgggggaagc gaagcaggcc gccgaccgtc gcaggccgcc tgccggtcat gcgtagggac
     6361 agggggcggt agggtggagc ggcgggggcc tgtcctggtg gccatacgca atacgagctg
     6421 gcctgtcaag ggcctccacc aggacaggc
//
LOCUS       DASZAK010000306         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_575724, whole genome shotgun sequence.
ACCESSION   DASZAK010000306 DASZAK010000000
VERSION     DASZAK010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575724"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..853
                     /locus_tag="VGT44_05380"
     CDS             158..853
                     /locus_tag="VGT44_05380"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2580265.1"
                     /translation="MEQTRRERQYLATQEEIKGVARRLMAEHGTAGLSTRMIAREMGL
                     TAPALYHYFASRDELITALIVDAFNALADALELARDRAAAEPAREQLTQTLLTYRQWA
                     IEHPIDFQMIYGNPIPGYEAPRELTVPAAARGFMILVPLAARLMHDDNRHASNPAREG
                     DAIPQSVQANLTEMRARDGYDVPLEVLYGAVSGWTRLHGIIMLELFHHLQPIVGDVDA
                     FYRAEIESILSTY"
     gene            866..1810
                     /locus_tag="VGT44_05385"
     CDS             866..1810
                     /locus_tag="VGT44_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2580266.1"
                     /translation="MSNSNDLYIVFGSGPVGHAVTRELVARGRKVRLVNRRGRPDAPE
                     GVELIQGDASDSAFTREACKGAAVVYNCLNAPYTQWPELFPPLQAGVLEGAAASGAKL
                     VVMENVYMYGSTRGKPITEDLPYAATTRKGRVRAQMAQGLLAAHRSGKVRVVIGRASD
                     FFGPGALQSSSGERMFLPPLTGKAAQIVGKPDLLHTYTYVPDIGKGLVILGEHDEAFG
                     QVWHLPSAQTVTTREFINMIFAETGYSPRIQSVSNLVLKGLALFNPIIREVAEMLYEF
                     DEPFVVDHSRFAQTFGNHATPLREAIHTTVEWYRKQNG"
     gene            1923..2201
                     /gene="mftB"
                     /locus_tag="VGT44_05390"
     CDS             1923..2201
                     /gene="mftB"
                     /locus_tag="VGT44_05390"
                     /inference="COORDINATES: protein motif:HMM:TIGR03967.2"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HEV2580267.1"
                     /translation="MPQSPPTYQLITEIKIRRESFGGILYKYGCADRGALSFINSPQL
                     IELLLAGQQRYEGNLNAAIEQSAYSAASRQKLYRALDDLVRRGYVLVQ"
     gene            2237..3376
                     /gene="mftC"
                     /locus_tag="VGT44_05395"
     CDS             2237..3376
                     /gene="mftC"
                     /locus_tag="VGT44_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03962.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HEV2580268.1"
                     /translation="MPEPIRFLGRQSHIALHGGVIGDIHPSALVAMQQTGRQEQPHGG
                     RGSQRFLQGGLSAPITVTWEITAACNIACVHCLSASGKRRPRELTTEQSLALVDELAE
                     MQVFQIHFGGGEPFIYPGIWQVLERSRERGLVMCVSTNGTLITPERARRLAVLEPIYF
                     QVSLDGGTPATNDRIRGQGVFQRALRGLERLATENLSLTVNSVLTRYSFAELDRLYDI
                     AASFNAKLRVTRLRPSGRGQEVWGELHPTREQYRAFAGWLTEHPDVLTADSFFHLNAF
                     GQKLAGLDMCGAATATCCICPEGEVYPCAFFQAPEFEAGNLHEHRFQDIWRSSALFDF
                     YRKMGGGACSGCGQYNTCHGGCPATKWFVQRSLQIRDPECVLQSY"
     gene            3405..>3737
                     /locus_tag="VGT44_05400"
     CDS             3405..>3737
                     /locus_tag="VGT44_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2580269.1"
                     /translation="MPTTGFVEIYGAPLYYEVAGDGPALVLIHEGIADSRMYDDQFDA
                     FAQQYRVVRYDLHGFGKSGAPEQAYTHHGALHGLLRHLGIERAALMGMSLGGITALDF
                     ALSYPEMVN"
BASE COUNT          770 a         1071 c         1119 g          777 t
ORIGIN      
        1 cttcgctgcg ctcaggatga cattcccgac tttggtcatg aaacatcgtt accatcgccc
       61 tggaatactt gacaaacgaa attaaacgat gtatattaat tttaacaatg ttaaataaaa
      121 caaacatcgt taaaacaaca gcaaagtgag gcatgatttg gaacagacac gacgagaaag
      181 gcaatacctt gccacacagg aggaaatcaa gggggtggcg cgccgtttga tggcggagca
      241 tggcacggcg ggactctcta cgcgcatgat cgcgcgcgag atggggctga ccgcgcccgc
      301 gctgtatcac tacttcgcca gccgcgacga gctgattacg gcgctgatcg tggatgcctt
      361 caacgcgctg gcggatgcgc tggaattggc gcgcgaccgg gccgcggctg aaccggcgcg
      421 ggagcaactg acgcagacgc tgctgaccta tcgccagtgg gcgattgagc atcccatcga
      481 cttccagatg atctacggca acccgatccc tggctacgaa gcgccgcgcg aattgaccgt
      541 tccggcggcg gcgcgtgggt tcatgatcct tgtgcctctg gccgctcgat tgatgcatga
      601 tgacaaccgg cacgcgagca atccagcgcg agagggcgat gcgatcccac aaagcgtgca
      661 ggccaatctt acggaaatgc gcgctcgtga tggatatgat gtcccgctag aggtgctgta
      721 tggcgctgtt tccggctgga cgcgcctgca tgggattatc atgctggaat tgttccatca
      781 cctgcaaccc atcgttggcg atgtcgatgc tttttaccgc gccgaaatcg aaagcatact
      841 atcaacctat tgaaaggaca aacgaatgag taactccaat gatttataca tcgtcttcgg
      901 aagcggcccg gttggacacg ctgtgacgcg ggaattggtg gcgcgtggtc gaaaggtgcg
      961 cctggtgaac cggcgtggca ggccggatgc gccggaaggt gttgagctga ttcagggtga
     1021 tgcctccgac tctgcattta cccgtgaagc ctgcaaagga gcagcagtcg tctacaactg
     1081 tctcaacgct ccgtacaccc agtggcctga actttttcca cccttgcagg cgggcgtgct
     1141 tgaaggagca gccgcatccg gcgcgaaact ggtcgtgatg gagaacgtct atatgtatgg
     1201 ctctacgcga ggcaagccaa ttacagagga tttgccttat gccgccacta cgcgcaaggg
     1261 cagagtgcgc gcgcaaatgg cgcaagggct gttagcagct caccgcagcg gcaaggtacg
     1321 agttgtgata ggccgcgcct ccgacttctt cggccctggg gcgcttcaat catcttctgg
     1381 agagcgcatg ttcctgcccc cattaaccgg caaagcagcg cagatcgtag gcaaacctga
     1441 ccttttgcat acctacacct acgtccctga catcggcaaa gggctggtga ttctgggcga
     1501 gcatgacgag gctttcggcc aggtatggca cctgcccagc gcgcaaaccg tgacaacgcg
     1561 cgagttcatc aatatgatct ttgccgagac aggatattca ccgcgtatcc agtcggtgtc
     1621 caacctcgtc ctgaaagggc tggcgctctt caatcccatc attcgtgagg tcgcagagat
     1681 gctctacgag ttcgatgagc cgttcgtcgt cgatcattcg cgctttgcgc agacctttgg
     1741 caaccacgcg acgcctctac gcgaggcgat tcatacgacg gtggagtggt atcggaagca
     1801 gaacggctag tttgacatgg cgaccgcgag ggtcgccatg gaaaaagctt gactcttcat
     1861 attttcctgg gtatgcttcc cattgtaaag gctgcagttg tatcttttcg aggagcaagc
     1921 ctatgcctca atctccgccg acgtaccagc ttattaccga gattaagatt cgccgcgagt
     1981 ctttcggggg cattctttat aaatatggat gcgccgaccg tggcgcgctc tcgttcatca
     2041 attcgccgca gttgatcgaa ctactgctgg cggggcagca gcgttatgaa gggaatttga
     2101 acgcggccat tgaacaatcc gcttattccg ccgccagcag gcagaagctc tatcgcgcgc
     2161 tcgatgatct tgtgcggcgc ggttatgtgc tggtgcaatg aaaatgtcct gaaacaatgt
     2221 aaagggaggc aacccgatgc ctgaaccaat ccgctttctg gggcgacaat cgcacatagc
     2281 ccttcatggc ggcgtcatcg gcgatattca cccatcagcg cttgtggcga tgcagcagac
     2341 ggggcggcag gagcagccgc atggcgggcg cggctcgcag cgctttttgc agggcgggct
     2401 gtccgcgccg atcaccgtca cgtgggagat cacagcggcg tgcaatattg cctgcgtgca
     2461 ttgcctgtcg gcgtcgggca agcggcgacc gcgcgagctg acgacggagc aatcgctggc
     2521 gctggtagat gagctggcag agatgcaggt ttttcagata cactttggcg gcggggagcc
     2581 gtttatttat ccgggcatct ggcaggtgct ggaacgcagc cgggagcgcg ggctggtgat
     2641 gtgtgtcagc accaacggca cgctcattac gccggaacgc gcccgccgcc tggccgtgct
     2701 cgagccgatt tatttccagg tcagcctgga tggggggacg cccgccacta atgaccgcat
     2761 ccggggccag ggcgtcttcc agcgcgcctt gcgcggcctg gaacgactgg ccaccgagaa
     2821 cctgagcctg accgtcaaca gcgtcctgac gcgctacagc ttcgcggaac tggatcgcct
     2881 ctacgacatc gccgccagtt tcaacgccaa attgcgcgtg acgcgcctgc gcccctctgg
     2941 acgtgggcag gaggtctggg gggaactgca tcccacgcgc gagcagtacc gcgccttcgc
     3001 cggctggctg accgagcatc ccgatgtgct gaccgccgac tcgttctttc acctcaacgc
     3061 ctttggtcag aagctggcgg gcctcgacat gtgcggcgcg gccaccgcca cgtgctgcat
     3121 ctgtcccgaa ggagaagtct acccctgcgc cttcttccag gcccccgaat tcgaggcggg
     3181 caacctgcac gaacaccgtt ttcaggacat ctggcgcagc tctgcgctct tcgacttcta
     3241 tcgcaagatg ggcggcggcg cctgcagcgg ctgcggccag tacaacacct gtcacggtgg
     3301 ctgccccgcc accaagtggt tcgtccagcg cagtttgcag attagagacc cagaatgtgt
     3361 gctgcaaagt tattagctca ttcacgcgac gaaaggctaa cctcatgcca actactggct
     3421 ttgttgagat atacggcgcg cctctctatt acgaggttgc aggagatggc ccggcgcttg
     3481 tgttgattca cgagggcatc gcggacagcc gcatgtatga tgaccagttt gatgccttcg
     3541 cacagcaata ccgcgtagtg cgttatgatc tgcatggatt tgggaagtcg ggcgcgcccg
     3601 aacaagccta tacgcatcac ggagcgctgc atggcttgct gcgacacctg ggcatcgaac
     3661 gcgcggcgct gatgggcatg tcgctgggcg gcatcaccgc tcttgatttt gctctgagct
     3721 atcctgagat ggtgaac
//
LOCUS       DASZAK010000307         5011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_647184, whole genome shotgun sequence.
ACCESSION   DASZAK010000307 DASZAK010000000
VERSION     DASZAK010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5011
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647184"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /locus_tag="VGT44_05405"
     CDS             complement(<1..1848)
                     /locus_tag="VGT44_05405"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2580270.1"
                     /translation="MNEPVGTGWESGIIEPDEASAANVGIEAFAARYTFPLDGFQIEA
                     MQQIDAGQSVMVVAPTGTGKTLVAEFAIWRAQQRGQRVIYTTPLKALSNQKYRDMRAL
                     YGQDAVGLVTGDIVEHSAASIVIMTTEVYRNMLLEEGSERRVSSTGNDELADVAFIIF
                     DELHFLSDIERGPVWEEAIVCSPPHIQLVGLSATVSNARDLADWISKVHRSISLVIHE
                     ERAVPLEHYYFLDNRLRLVMGADDQRVERFPRVGGEARIARMRGQNRMYSFGDDETDG
                     AEAREEQDAANGAGKTGGRKAAARTQREATPLVEHSTPEPGEVLTALRQADLLPCLYF
                     LPGRRVVEDAAMGAALHHFTSPEEEALIREEVSVWMEHLPKEDRNLRQAHELANLLPR
                     GLAYHHAGLLPGLKVLVETLFQRGHLRAVFATDTLALGINMPARAVVVGSLSKFDGQE
                     MRLLTPNEYRQLTGRAGRRGMDRHGAAVIPYSAWEQFEETFQRITAELLPVTSSFVIR
                     YNSVLNLWRPGDMAHLRRICASSLREYQRYELWERREMKRLEREERQAQKGKKVGKKK
                     GAASDAAIEALEKRRKRIGDYPLSRAGAAELDGTVFALRALGYIDEQEQL"
     gene            complement(2057..2599)
                     /locus_tag="VGT44_05410"
     CDS             complement(2057..2599)
                     /locus_tag="VGT44_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580271.1"
                     /translation="MFRDFRSMNVFVLVAVLLLLAACGGADTSATSTPTVQSGTVAAS
                     TPGVGPIVAVTPTADAGTTPAQQTITLADRTLMLGQVSRSPGASASSTAVSITITVTD
                     TTAMAIQNQSSFYQLVGAEGDAFGVQSSVTPGFYGTIAAKQWHKGTITFQVPTGASQG
                     LQLLYRPEKATETTLIPLPK"
     gene            2800..3792
                     /locus_tag="VGT44_05415"
     CDS             2800..3792
                     /locus_tag="VGT44_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580272.1"
                     /translation="MKSQNVHDQENVSRASAISVSEREMLVNEETVELTYESGDGGTA
                     QSQPEEDHRPPRLAATGGLAVILGQLVAYFHHRANIDDLDVLAFSLHHLFCRIDFLYE
                     TALPRLAALADASISGQDGSQHAPYAQQQPTNHAQYVRHREQLWTQLQTINRTLDRME
                     PICHLLSDATECVLERFDASTDMLGFLDQEPGEPNTSEAAEQSDDINYFQMIDAERWD
                     HAFAAITRSLLNWQEQYGKLTPFTTQFAPLLQVIPLLPEIDAALAIILDSAGAIFGDI
                     LPGFRAILEADEAMIAALLYDLMQQSDHLLVKFDTTLEPMNMLIKYYAVGASLT"
     gene            4014..4862
                     /locus_tag="VGT44_05420"
     CDS             4014..4862
                     /locus_tag="VGT44_05420"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580273.1"
                     /translation="MGNEADVANYTASLPVRETTQALYTYDIIETLEPVRASINNLCL
                     EGIEQIALVAIRLQFDPDARHDLVIRSATHLLDNLRLLVRKTDQVFLHTQTMYFVLRA
                     ANQQGGEIVQTRLCEALLWRVHNMNEPELQRPRNVDIGHAGYVEEGSVEATIETSCAW
                     QLHYEWHPEKSRKASRQSQAEQMPIADPLDIELPVLARKLGIPYLSLLPRKLPINLQR
                     LVKPSLAQELRCFPIGRERDTLTVAMLDPRDLDALNRLRAETGLHIFPVLAHPEALQT
                     ALEQLI"
BASE COUNT         1101 a         1587 c         1279 g         1044 t
ORIGIN      
        1 caactgctcc tgctcgtcaa tataaccaag tgcgcgcaga gcgaagacgg taccgtccaa
       61 ctcggctgcc ccggcgcggc tgagtggata gtcgccgata cgcttgcgcc gtttctccaa
      121 cgcctcgatt gccgcgtcag aagccgcgcc tttctttttt ccgaccttct tgcccttctg
      181 cgcctgtcgc tcctcccgct ccaaccgctt catctcgcgc cgctcccaca gctcgtagcg
      241 ctggtattcg cgcaggctgg aagcgcagat gcgccgcagg tgcgccatat ccccaggtcg
      301 ccacaagttc aagacggaat tatagcgaat tacaaaagaa ctggtgacgg gcaacaactc
      361 ggcggtaatc cgctggaacg tctcctcgaa ttgctcccag gccgaatagg ggatcaccgc
      421 cgcgccgtgc ctgtccatgc cgcgccggcc cgcgcgcccc gttagctgac gatattcgtt
      481 aggggtcagc aggcgcatct cctgcccatc gaacttgctc agactgccca ctacgacggc
      541 gcgcgcaggc atattgatgc ccagcgccag cgtatcggtc gcgaacaccg cccgcaggtg
      601 gccgcgctga aacagtgtct caaccagcac cttcaggccc ggcagcagcc ccgcgtggtg
      661 ataggccagc ccgcgcggca gcagattcgc cagttcgtgc gcctggcgca aattgcgatc
      721 ttctttgggc agatgctcca tccagacgct gacctcttct cgaatcaacg cctcttcttc
      781 cggcgaagta aagtggtgca gcgcagcgcc catcgccgcg tcttccacca ccctgcgacc
      841 cggcaaaaaa tagagacacg gcagcagatc ggcctggcgc aaagcggtca gcacctcgcc
      901 tggttccggc gtggaatgct caacgagcgg cgtcgcctcg cgttgcgtgc gcgcagccgc
      961 ttttcgccca cctgttttcc ccgcgccgtt cgccgcgtcc tgctcctcgc gcgcttcagc
     1021 gccatcagtt tcatcatcgc cgaagctata catgcggttc tgcccgcgca tcctggcgat
     1081 gcgcgcttcc ccgcccactc tcgggaaacg ctcaacgcgc tggtcgtcgg cgcccatcac
     1141 caaccgcagc cggttatcca gaaagtagta atgttcgagg ggcaccgccc gctcctcgtg
     1201 gatcaccagc gagatcgagc gatgaacctt actgatccaa tccgccaggt cgcgcgcgtt
     1261 gctgaccgtc gccgacaaac cgacgagctg gatatgcggc ggcgaacaaa cgatggcctc
     1321 ctcccacacc gggccgcgtt cgatatcgct caggaaatgc aactcatcga agataataaa
     1381 cgccacgtcc gccagctcgt cgttccctgt agaggacacg cggcgctccg acccttcctc
     1441 cagtaacata ttgcggtaga cctcggtggt cataatcacg atcgaggcag cgctatgctc
     1501 aacaatatcg ccggtcacga gaccaaccgc gtcctggcca tacagcgccc gcatatcgcg
     1561 atatttctga ttcgagagcg ccttgagcgg ggtcgtataa atcacgcgct ggcctcgctg
     1621 ctgcgcccgc cagatagcaa attccgccac cagcgtcttc cccgtgccgg tcggcgcaac
     1681 caccatcacc gactgccccg cgtcaatctg ctgcatcgcc tcaatctgaa aaccatccag
     1741 gggaaaggta taacgcgcgg cgaacgcctc gatacccaca tttgctgcgc tcgcctcatc
     1801 gggttcgatg attcccgact cccacccggt tcccaccggc tcattcataa tgtgtattgc
     1861 ttccttttac gtctatcttc atggtagctt attaattcga actacctgct tactgtccgt
     1921 aatgctccag gcgaccccct gacgcactac tagattctct cctgctgcaa aaccttaagc
     1981 acactataca acatatcaaa gaacatcttt cacctgtgtc ctcttcccta tttgcctccc
     2041 ccgcccgtac agacgatcac tttggcagcg ggatcagtgt tgtctctgtc gccttctctg
     2101 gccggtagag cagttgcaga ccttgcgacg cgccggttgg aacctggaac gtgatcgtcc
     2161 ccttatgcca ctgtttggca gcgatcgtgc cgtaaaagcc gggagtgacg ctcgactgga
     2221 caccaaaggc atcgccctct gcgccgacaa gttgatagaa cgaggattgg ttctgaatgg
     2281 ccattgcggt cgtatcggtc acggtaatcg tgatgctgac cgccgtcgag gaggcgcttg
     2341 cgcccggcga acggctcacc tgtcccagca tcaacgtgcg gtccgcgagt gtaatggtct
     2401 gctgcgctgg tgtcgtcccc gcatcggctg tcggcgtgac cgccacaatc ggcccaacac
     2461 ccggtgtgct ggcagccacc gtccctgact gaaccgtcgg tgtggacgta gcgctggtgt
     2521 ctgctccccc acatgcagct agcaatagca gcactgcgac aagtacaaaa acattcattg
     2581 agcgaaagtc acgaaacata acgattattt ttccctacat agacaaaaga aggtggccac
     2641 cttcttttgc gaatctccat actctattag cgctgatgcc ttcgttatca tgcattatca
     2701 tgaacaggtt tctcgagcgc aagtccctgg taccattagg ttctaccggt acaatagaaa
     2761 gtaaggaaca tcaataagtg gtgatgggag gggattggta tgaaaagtca gaacgtccat
     2821 gaccaggaga atgtgagtcg cgcgtcggct atctccgtat ctgaacggga aatgctcgtc
     2881 aacgaggaaa ccgttgagct gacgtatgaa agtggagatg gtggcacggc gcaaagccag
     2941 ccggaggagg accatcgccc ccccaggctg gctgccactg gcggcctggc agttatcctc
     3001 ggtcaactgg ttgcctactt tcatcatcgc gccaatatcg atgacctgga tgtgctcgcg
     3061 ttcagtctgc atcacctgtt ctgccgcatc gattttctct acgaaaccgc gctgccacgg
     3121 ctggcagctc tagcagatgc gagcatatct ggccaggatg gttcacaaca tgcaccgtat
     3181 gcgcaacagc aaccgaccaa ccatgcccaa tacgtgcgtc accgcgagca gctatggacg
     3241 caattacaga cgatcaaccg cacgctggac cgcatggagc caatttgcca cctgctgagc
     3301 gacgcaacag aatgcgtcct tgaacgcttt gacgccagta ccgacatgct tggctttctc
     3361 gaccaggagc ctggagaacc aaatacaagc gaagcagccg agcagagcga tgatatcaat
     3421 tacttccaga tgatcgacgc ggagcgttgg gaccacgcct tcgccgccat cacccgcagc
     3481 ctgctgaact ggcaggaaca atacggaaag ctgaccccgt ttacaacaca attcgctcct
     3541 ctgctccagg tcattccctt actgcctgaa atcgacgcgg ccctcgcaat cattctagat
     3601 agcgcgggcg ctatcttcgg tgatatcttg cccggcttcc gcgccatcct ggaggccgat
     3661 gaagccatga tcgcggcatt gctttatgac ctgatgcagc aatcggatca cctgctggtg
     3721 aaattcgata caacattaga gccaatgaac atgctgatca aatactacgc cgtcggcgcc
     3781 agtctgacct gattgataga attgtattag gagttatctt aaccaccacg cgaaggtctt
     3841 aacaagaacg caaccatttt accgcttact tcctaacata cctcgtgtaa gatgaacaaa
     3901 tacagtacat gacagtgcgg gttccagagg gcgtaccctt gcggtcgccc tcttggtatt
     3961 gaccagtccc atttcgatat tgaccagtcc tggtgcaatg aaggaataca aggatgggca
     4021 acgaagctga tgtagctaac tatactgcat ctctcccggt gagggaaacg acgcaggcgc
     4081 tctacacata tgacatcatc gaaacactcg agccagttcg ggcttcgata aataatctgt
     4141 gcttagaggg catcgaacag attgccctcg tcgctattcg tcttcagttt gacccggatg
     4201 ccaggcatga cctggtgata cgttccgcga cgcatttgct cgacaacttg cgcctcctcg
     4261 tgcgcaaaac cgatcaggtc tttttgcata cccagaccat gtacttcgta ctgcgggccg
     4321 ccaatcagca gggcggcgaa attgtgcaaa cccgtctttg tgaagccttg ctctggcgcg
     4381 ttcataatat gaacgagcca gaattgcaga ggccgcgcaa tgtcgatatc ggccacgctg
     4441 gctacgtaga ggagggaagt gtcgaggcca ccatcgagac ctcctgcgcc tggcaattgc
     4501 actatgaatg gcatcccgaa aaatcgcgca aagcctcccg gcagagccag gcagaacaaa
     4561 tgccaatcgc cgatccactc gacatagagc tgccagtcct ggcacgcaag ctgggcatac
     4621 cttatctgtc gctcttgcca cgcaagctgc ccatcaacct gcagcgcctg gtcaagccca
     4681 gcctggcgca agaactacgc tgcttcccaa taggccgcga gcgcgacacc ctgaccgtcg
     4741 ccatgctcga tccccgcgac ctcgacgctc tcaaccgcct gcgagcagag accgggctgc
     4801 acatcttccc cgtgctggca caccctgagg cactgcaaac cgcgcttgaa caattaatat
     4861 aaacgcgata caatgggaat catagtattc taacaaggtt caatcgacca gtcctgatga
     4921 cccgtaggag ccgatttatc gcgctcaccg ccgattgatc ggccagtcta catgcctgat
     4981 cgacgaattc ctggccgatc aatcggcggt g
//
LOCUS       DASZAK010000308         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_540312, whole genome shotgun sequence.
ACCESSION   DASZAK010000308 DASZAK010000000
VERSION     DASZAK010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540312"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..126)
                     /locus_tag="VGT44_05425"
     tRNA            complement(55..126)
                     /locus_tag="VGT44_05425"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Lys,seq:ttt)
     gene            303..686
                     /locus_tag="VGT44_05430"
     CDS             303..686
                     /locus_tag="VGT44_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2580274.1"
                     /translation="MSKQILIVDDKSELLHLMRRILEDEQYQVSILQDGKDTFTMMKT
                     RLPDLLILDLKLGDISGKDILSQLKGDPTTSEIPVIVYTAAVIEAEEVNRLIANDPDY
                     YQRVYLIQKPFELPHLLDMVEQVVN"
     gene            868..1158
                     /locus_tag="VGT44_05435"
     CDS             868..1158
                     /locus_tag="VGT44_05435"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV2580275.1"
                     /translation="MKFLLGLLFGFALGVAAGLLMAPQTGEETRNQLNEQGVMLRDRS
                     SGLSEQVLGRATGLTEQVRNRATDALSQGKELYQRTKNELAEQYSKARSGEA"
     gene            complement(1234..1476)
                     /locus_tag="VGT44_05440"
     CDS             complement(1234..1476)
                     /locus_tag="VGT44_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580276.1"
                     /translation="MPTGDEKLTALLADLREMASYLHGQGEKKLALARRFEEDAKKDA
                     ANREFDLSQAKMLDYQHYIWHEIANMVEKLVKQYEK"
     gene            complement(1476..1730)
                     /locus_tag="VGT44_05445"
     CDS             complement(1476..1730)
                     /locus_tag="VGT44_05445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580277.1"
                     /translation="MTEELLDALYHIDKHSHLFTARERAALRFAEIMTTGARDIDEAL
                     WDELQAHFDDGEIVELATVIGLFNFFNRYADALQLQEGGK"
     gene            complement(1767..2012)
                     /locus_tag="VGT44_05450"
     CDS             complement(1767..2012)
                     /locus_tag="VGT44_05450"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV2580278.1"
                     /translation="MSRIPPVAHFPFPGDDQPADAPFGMSAVSSMSTLFGTMAHRPEL
                     ARRSMALLEEAMRGGTVEPQIKELVAVRVSQVNHCFY"
BASE COUNT          519 a          610 c          626 g          526 t
ORIGIN      
        1 gcgccaggga ctgattccgc tggaggagtg gtaagggtgg attatgaatt gcagtgaggg
       61 cggaggggat cgaaccctcg gccagacgat taaaagtcgt ctgctctacc actgagctac
      121 gccctcaagc ctgagttcac gagcgtagta tatcatcacc gtcaagagtt tcacactttt
      181 ctcataaagg gcgaccgcaa tagtcgccct ttatgagaaa tccaacattt ttcttgcttg
      241 ttcgtgtcga acatgataca ctaccagaaa acaatctgaa ccgggaggcg agtcgagccg
      301 ttgtgagcaa acagatactg atcgttgacg ataaaagcga attgttgcac ctgatgcgcc
      361 gcatcctgga agacgagcaa taccaggtct cgatcttgca ggacgggaaa gacacgttta
      421 cgatgatgaa gacccggctg cccgatttgc tgattcttga cctgaagctg ggggatattt
      481 cgggaaaaga tattttaagc caactgaagg gcgatccaac cacatcggaa atccctgtga
      541 tcgtctacac agcggcggtc atcgaggccg aagaggtcaa ccggctgatc gccaacgacc
      601 ccgactatta tcaaagggtc tatctgattc aaaaaccctt tgagcttcca cacctgctgg
      661 atatggttga gcaggtggtc aactagcctc gcgatcaagg caacctttca gggccttcat
      721 acgtattgta ttgtgtgcct gagcaggcat gttggaaacc agtaggccgg taacgagtag
      781 ttacagcagg caatcgtagg cagtcgtgac tcgtagcaac gtaaacggac gatagtgggt
      841 accgtatgtg aaaggagaag gagaaatatg aagttcctct taggcttact ttttggcttt
      901 gcattaggcg tggcagcggg gctgctgatg gcgccacaaa cgggtgaaga aacccgcaat
      961 cagttgaacg aacaaggcgt catgttgcgc gatcgttcat cgggcttgag cgaacaggtg
     1021 cttggacgtg ccaccggcct gaccgagcag gtgcgcaatc gcgccactga tgcgctctcg
     1081 cagggcaaag aactctacca gcgcacgaag aacgagctgg cggagcagta cagcaaggct
     1141 cgctctggcg aggcatagga acacgcggcg cagcacagcg cccatatttg cggccaggac
     1201 gatcagcgcg attgccctgg ccgcttattg cgcttatttt tcgtattgtt tcaccagttt
     1261 ttcaaccatg ttagcaattt cgtgccagat gtagtgttga taatcgagca tcttcgcctg
     1321 gctgagatcg aactcgcggt tcgcggcatc tttctttgca tcttcctcaa agcgcctggc
     1381 gagcgccagc ttcttttcgc cctggccgtg caggtagctc gccatctcgc gcaaatctgc
     1441 cagcagcgcc gtcaatttct catcgccggt aggcattatt tccctccctc ttgcagttgc
     1501 agcgcatcgg cgtagcgatt gaagaaattg aacaggccga ttaccgttgc aagctccacg
     1561 atctcgccgt cgtcgaagtg cgcctgtagc tcgtcccaca gggcctcgtc gatgtcgcgc
     1621 gctcccgtcg tcatgatttc cgcgaaacgc agcgccgctc gttcgcgcgc cgtgaacagg
     1681 tgactatgct tatcaatgtg atagagcgcg tcaagcagtt cttccgtcac acccagctgc
     1741 tttgccaaag cagtatgcgc cgacattcaa tagaagcaat gattgacctg cgaaacgcgc
     1801 actgctacca actctttgat ctgaggctca accgtcccgc cgcgcatggc ctcttcgagc
     1861 agcgccatac tgcgccgcgc caactcaggc cggtgcgcca tcgtgccaaa cagcgttgac
     1921 atggatgata cggctgacat cccaaacggc gcgtcggcgg gctggtcatc gccgggaaac
     1981 gggaaatgcg ctacaggtgg tatgcgcgac atgtcgggtt tgcttctttc gttgtgttga
     2041 taaatgagta tgcgagccag gtaatagagc gacctggctc gcgcatgata ctatttgctc
     2101 tatatacgtc attattgtag tggacaaagg gggaacgcgc aatagacctt ttcatcctgc
     2161 cgcttatttt actgccgttg gggcttgata cgctggcgat ctcgattggc ctcggcatca
     2221 aaagcgcgtt gcctgtcgcc tcacccgccg ggagacctgt atttccacat tggctgcgtt
     2281 c
//
LOCUS       DASZAK010000309         1923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_575763, whole genome shotgun sequence.
ACCESSION   DASZAK010000309 DASZAK010000000
VERSION     DASZAK010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575763"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VGT44_05455"
     CDS             <1..265
                     /locus_tag="VGT44_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV2580279.1"
                     /translation="VDEEIKVPTLYIDRVGERAHLDRLNRVRRERDQSAAQRALANLR
                     RAAEGTQNTMPAIIEAVRAYATLGEIMQVFREVFGEYMEPAVF"
     gene            265..996
                     /locus_tag="VGT44_05460"
     CDS             265..996
                     /locus_tag="VGT44_05460"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2580280.1"
                     /translation="MRLAVISDMHGNAIALDAVLAEIKTEGIDHIVCLGDVAATGPQP
                     RQVIERLKALGCLVVMGNTDAWLLSPEIKETGDEFSRKIEDIDLWCAQQLSAGDKAYL
                     QTFQPTITYALERSKSLLGYHGSPLSFHEQILPITPQEDLDQAFAGCHANILAGGHTH
                     LQMFRRYRDMLIINPGSVGLAYDPVYPLDEARNPPWAEYAVVNVTEGKLSVELRRTPF
                     DVRTFAETIVESGMPHAEWLAKDWG"
     gene            1000..1866
                     /locus_tag="VGT44_05465"
     CDS             1000..1866
                     /locus_tag="VGT44_05465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2580281.1"
                     /translation="MPDIVTRATLTTAEIASIEQLIAICNRHDNLQMRIGPGMLREQM
                     GEAKSNFLYYEDGKLAGYLWSDSFGTREKELVGMVHPAYRRQGIFRALLDSAKATYRQ
                     LGIEQLIFICEHSSQSGLACLSALGARYSFAEHKMMLGTFHERGHVIEGLHMRQAGED
                     DIDAIVTLLATDTGNVVDTLAWVSYLMGQPQYHFYLATLHSKPLGTLRLDFLEEEHED
                     WIYAFEVRLGYRGLGYGRQMLEQAIHIARAVNNYPILLDVETMNTNAIGLYQSCGFEI
                     KTTYDYYELPIS"
BASE COUNT          445 a          581 c          510 g          387 t
ORIGIN      
        1 tgtagacgag gaaatcaaag tcccgaccct ctatatcgac cgcgtcggcg agcgcgccca
       61 cctcgaccgc ctcaatcgtg tgcgtcgcga acgcgaccag tccgccgcgc agcgcgccct
      121 cgccaacctg cgccgcgccg ccgagggaac gcaaaacaca atgcccgcca tcatcgaagc
      181 cgtgcgcgcc tacgccaccc tcggcgagat catgcaggtc ttccgcgaag tgtttggcga
      241 atacatggaa ccggcggtgt tttaatgcgc cttgcggtta tctccgatat gcatggcaac
      301 gccatagcat tagatgcggt actggctgaa atcaagacag aaggaatcga ccatatcgtc
      361 tgcctgggtg atgtcgcagc aaccggaccg cagccgcgcc aggtcatcga acgtttgaaa
      421 gctcttggtt gcctggtagt gatgggtaat accgacgcct ggctgctttc acctgaaatc
      481 aaggaaaccg gcgatgaatt ctcgcgcaaa atcgaggata tcgatctctg gtgcgcgcaa
      541 caactctccg ctggtgataa agcatatctg caaacgttcc aaccaactat cacctacgct
      601 cttgagagga gtaaatcctt gctcggttat catggctcgc ctctctcgtt tcatgaacag
      661 atactgccaa taacgcccca ggaagacctg gaccaggcgt ttgcaggctg tcatgccaac
      721 atcctggccg gtgggcatac gcacttacag atgttccggc gctaccggga catgttgatt
      781 atcaaccccg gcagcgttgg cctggcctac gaccccgtct accccctgga tgaagcgcgc
      841 aatcctcctt gggccgagta tgctgtggta aacgtcacgg agggaaagtt aagcgtagag
      901 ctgcgccgca ccccgtttga cgtgaggacg tttgctgaga caattgtgga gagtgggatg
      961 cctcacgctg aatggctggc gaaggattgg ggctaaatca tgccagacat cgtcacacgc
     1021 gccacgttaa ccactgccga aatcgcctcc attgaacagc tcatcgccat ctgcaaccgg
     1081 catgataact tgcagatgcg catagggcca ggaatgctgc gcgagcaaat gggagaagcg
     1141 aagagcaatt ttctctatta cgaagatggc aagcttgctg gctacctgtg gagcgacagc
     1201 tttggcacgc gagagaaaga actggtcggc atggttcacc cggcctatcg ccgccagggc
     1261 atctttcgcg cgctgctcga ctccgcaaag gccacctacc ggcagctcgg catcgaacaa
     1321 ctcatcttca tctgcgaaca ctcctcgcaa tccgggctgg cctgcctgtc agcgttgggc
     1381 gcgcgttact ccttcgcgga gcataaaatg atgctgggaa cgttccacga acgcgggcac
     1441 gtcatcgaag ggctgcacat gcgtcaggca ggcgaggacg acatcgatgc catcgttacc
     1501 ctgctggcca ccgacaccgg caacgtcgtc gacacgctcg cctgggtctc gtacctcatg
     1561 ggacagccac aataccattt ctacctcgcc accctgcata gcaaaccgct cggtacgctg
     1621 cgtctcgact tcctcgaaga ggaacatgaa gactggattt acgccttcga ggtgcgtctg
     1681 ggctatcgcg gcctgggtta tggtcgccaa atgctcgaac aggccatcca catcgcccgc
     1741 gccgtaaaca actatcccat cctgctcgat gtcgaaacga tgaacacaaa cgccatcgga
     1801 ttgtaccaat cctgcggctt cgaaatcaaa acaacctatg actattatga attaccaatt
     1861 tcttaggtgc cgattatgaa ctttcacaac cagggccgca ctgtcatcct gagcgcagcg
     1921 aag
//
LOCUS       DASZAK010000310         4591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_7793, whole genome shotgun sequence.
ACCESSION   DASZAK010000310 DASZAK010000000
VERSION     DASZAK010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7793"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..2281
                     /locus_tag="VGT44_05470"
     CDS             269..2281
                     /locus_tag="VGT44_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV2580282.1"
                     /translation="MTSTFPPRILYGADYNPEQWPESVWQEDMRLMQLARVNMVSINI
                     FSWAMLEPRPREYHFETLDRIMDMLAEHGIAADLATATASPPPWMSRLYPAMLPVTWD
                     GRRMSHGSRQHYCPNSLDYRRACAELARRLAGRYRAHPALAMWHVNNEYGCHIPGCCC
                     DRCALVFRDWLQERYATLDALNEAWSTNFWSQRYSDWEDILPPRLTPAFNNPGQVLDY
                     HSFMSDSLLQCFLTEAHILREITPAIPVTTNFMAAYKPVDYFDWASHLDIASIDLYPA
                     HTAQSWETAITYDLMRSLKRGQPFLVMEQTPGQVNWMAQNAQKRPGNLRLQSHQALAH
                     GADGVMYFQWRQSRGGAEQFHSAVVPHEGSEHGRIFQQAAHIGAELERLSPAIVGSRM
                     QAQVALVMDWQNWWAVENPPRPSDRLRYWEQIRTWYRALHRLNVAVDFVPPEGDLSAY
                     RLVFAPLLHLLRPGAAQNLERFVSHGGTLLTTFFSGIVDQYDRVVLGGYPAELRKLLG
                     IHVEEFDPWTPQMTNEIVIQEGPLRGNYPCTLWGEVVHLEGAQALGVFASDYYAQGPA
                     LTVHRFGAGQAYYVATHGDDELVDRLTHLLCQQAAISPVLDVPEGVETTARKQADGHI
                     LYFLLNHNETAAQVTLPPGTFTSLLTGGKVEGQLEIAARDVVVLRA"
     gene            complement(2283..3317)
                     /locus_tag="VGT44_05475"
     CDS             complement(2283..3317)
                     /locus_tag="VGT44_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580283.1"
                     /translation="MMNTMMALWNRGWRGRLSVMLLVFFSLCISISLLVITAGSAWGA
                     LFVHRPQANVPAGVTGTNGLTSMAGTTAISTGSTVQSAQLTATATSVQIGVTNPCFVT
                     VTSAGMQPTVGRPTPKASATRSGQPGGGRPRVTPTPPHPRLTPTPTRQPSPTSSPFPT
                     PSASPSPVVTVTVVTTPIPTITPGVTPTLIPTPTLLPTPSPFPTITPTPLPTVTVTPV
                     ITPTDTSTPGATPTVTPFLTPTDTATPGASPTVSPALSPTVNIGLTAMPTLTWTPIGS
                     QSSGGDTLPGNPSSSGAAGSCLGDTLLTGSFSTLMLSLQLEAWVSLCASLAGTVICCG
                     TIARRARRAL"
     gene            complement(3430..4368)
                     /locus_tag="VGT44_05480"
     CDS             complement(3430..4368)
                     /locus_tag="VGT44_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580284.1"
                     /translation="MQDEQALVVCAFGWGKEFRLYREMLYVGSRGYRLTGLTQVQLKT
                     QQTLGVASASLDLRFGRETVALRGIAAVDDARRVAEYLVSWCGEGQKTGPRLSSRGET
                     APLKQAELTQRTWQPLPSPIPQTLTLHEQVTRPVDTPQLLSFGKDPQTGPKHNWKQER
                     KTERLAQAEVLPVINVPVRLESGEQAYYCSRATRCGELIKETLRSTYPAQDHGLLILT
                     DRRLLFIGRKSQLVLEYAHLLRISRLEGALSFETDHWQKRAIFTTPQPDLCIARLEAI
                     LQTRAAARQPGTGPVTPRVAAYAPGVAQRAERVKTM"
BASE COUNT          900 a         1453 c         1329 g          909 t
ORIGIN      
        1 gcaagggtct ccaccccgct ccgctccacc cccgccctta caaagaacga cggatcggcg
       61 gggcgagcgg cttcgaagtc tttgtaaggg cgggggtgga gcggagcggg gtggagaccc
      121 ttgcggtcgc cctcgtcttc catgccccga tggccaccac tcaaagaaag caaaggggag
      181 cggagcgggg tggagaccct tgcggtcgcc ctcgtcttcc atgcccctac gaacgatggc
      241 cgccactcaa ggaaaggaaa gcagaatcat gaccagcaca tttccaccgc gcatcctgta
      301 cggagccgac tacaaccctg agcagtggcc tgagtccgtc tggcaggaag atatgcgcct
      361 catgcagctg gcgcgcgtga acatggtctc gatcaacatc ttctcctggg ccatgctcga
      421 accacgaccg cgggaatatc acttcgaaac actggatcgc atcatggata tgctggcgga
      481 acacggcatc gccgccgacc tcgcaacggc gacggcctct ccgccgccct ggatgagccg
      541 cctctaccct gccatgctgc cggtgacatg ggacggcagg cgcatgagtc acggctcgcg
      601 ccagcactac tgcccgaaca gcctggatta ccggcgagca tgtgcggaac tggcgcggcg
      661 cctcgccgga cgttaccggg cgcaccccgc gcttgcgatg tggcacgtca ataacgagta
      721 tggatgtcac atccccggct gctgctgcga caggtgcgcc cttgtctttc gcgactggtt
      781 acaggagcgc tatgctaccc tggacgcctt gaacgaggca tggagtacaa atttctggag
      841 ccagcgctac agtgactggg aagatatcct gccgccgcgt ctcacgccgg cgttcaataa
      901 ccccggccag gtcctcgatt atcacagctt catgtccgac tcgctgctcc aatgctttct
      961 gaccgaagcg catatcttac gcgagataac ccccgccatt ccggtcacga cgaatttcat
     1021 ggccgcctac aagccggtgg attatttcga ctgggcatcc catctcgata tcgcctccat
     1081 cgatctctac cctgcgcata ccgctcaatc ctgggaaacg gccattacct atgacctgat
     1141 gcgttcactc aagcgaggtc aaccattcct ggtcatggag caaacgccgg gccaggtcaa
     1201 ctggatggca cagaatgcgc agaaacgccc aggcaatcta cgcctgcaaa gccaccaggc
     1261 gctggcgcac ggcgctgacg gcgtgatgta tttccagtgg cgtcaatcgc ggggaggcgc
     1321 ggagcagttc cacagcgcgg ttgtaccgca cgaaggcagc gagcatggcc gcatcttcca
     1381 gcaggcagcc catatcggcg ctgaactcga acgcctctct cccgctatcg ttggctccag
     1441 aatgcaggcc caggtggccc tggtcatgga ctggcaaaac tggtgggcag tcgaaaaccc
     1501 gcccaggccg agcgaccgcc tgcgctattg ggagcaaatt cggacgtggt atcgcgccct
     1561 gcaccgcctc aacgtggctg tggatttcgt cccgcccgaa ggcgacttga gcgcgtaccg
     1621 cctagtgttc gcaccgctcc tgcacctgct gcgtcccggc gcagcccaaa atctggaacg
     1681 cttcgtatca cacggcggta cgctgctcac aacctttttc agcggcatcg tcgatcagta
     1741 cgaccgcgtg gtgctgggcg gctatcccgc cgaactgcgc aagttgctgg gcattcatgt
     1801 cgaggaattc gacccctgga cgccgcaaat gaccaacgag atcgtcatcc aggaaggtcc
     1861 actgcgcgga aactacccct gtacgctctg gggcgaagtt gttcacctcg aaggcgctca
     1921 ggcgctcggc gtcttcgcca gtgactacta cgcccaggga cccgccctca ccgttcaccg
     1981 ctttggagca ggccaggcat attacgtcgc cacacatggc gatgatgagt tagtggacag
     2041 gttaacccac ctgctctgcc agcaagccgc catttctccc gttctggacg ttccagaggg
     2101 cgtagagacc acggcgcgca agcaggccga tggacatatc ctgtacttct tgctcaacca
     2161 caacgagacc gccgcgcagg taacgctacc acccggaaca ttcacctcgt tgcttaccgg
     2221 cggcaaggta gaggggcaac tggaaatagc cgcgcgcgat gtagtcgtgc tgcgcgctta
     2281 gctcaaagcg cccgtctcgc tcgccgcgcg atcgtgccgc agcatatcac cgtcccggcg
     2341 agcgaggcgc agaggctcac ccaggcttcg agttgcagcg agagcatgag cgtggagaaa
     2401 ctgcccgtca gcagcgtatc acccaggcaa ctccctgccg cgcccgacga tgacgggttg
     2461 ccggggagcg tgtcccctcc gctggactgc gaccctatcg gcgtccacgt gagtgtgggc
     2521 atcgctgtaa ggccaatgtt gaccgtcggc gaaagcgcag gtgaaacggt tggtgatgcg
     2581 ccaggcgtgg ctgtatctgt tggcgtcaaa aaaggtgtca ccgtcggcgt cgcgcccggt
     2641 gtgcttgtat cagtcggcgt tatcaccggt gtcactgtaa cggtcggtag aggcgtcggc
     2701 gtgatcgtcg gaaaaggcga cggcgttggc agcagtgttg gcgttgggat aagtgttggc
     2761 gtcacacccg gcgttatagt agggattggc gtcgtcacaa ccgtaaccgt tacaaccggc
     2821 gaaggcgatg ctgacggcgt gggaaatggt gacgatgttg gcgatggctg gcgcgtcggc
     2881 gtcggtgtaa gtctgggatg cggtggcgtc ggcgtcacgc gcggcctgcc tccaccgggt
     2941 tgtccactgc gtgtcgcgct cgccttcggc gttggtcttc ccactgtcgg ctgcataccg
     3001 gcgctcgtaa ctgtcacaaa acagggattg gtcacgccta tctgcacgct ggtcgcggtc
     3061 gccgtcagtt gagccgactg aacagtggac cctgttgata tagctgttgt gcctgccatg
     3121 ctcgtcaggc cgttcgtgcc ggtcacaccg gcgggaacat ttgcctgggg tctatgaacg
     3181 aacaacgccc cccacgcgct gccagccgta atcacaagta gggatatact gatacacagg
     3241 ctaaaaaaaa cgaggagcat cacactcaat cgccccctcc accctctatt ccagagcgcc
     3301 atcatggtat tcatcatctt ctcctatcct tcccccctga taccagttgg tacttcgttt
     3361 gtgccacccc cagggcgacc gcatgggtgg agacccttgc ggtcgccctc gtctctagtc
     3421 cgatgtccat tacattgtct tgactcgttc cgcgcgctgt gcgacgcccg gcgcgtatgc
     3481 ggccacacgg ggcgtgactg gacctgttcc cggctggcgc gccgcagccc gcgtttgtaa
     3541 aattgcctcc aatcgggcaa tgcagaggtc cggctgtggc gtggtgaaaa ttgcgcgttt
     3601 ctgccagtgg tcggtctcaa acgacagtgc gccctctagc ctggaaatac gcagcaggtg
     3661 cgcatattcc agcacaagct ggcttttgcg cccgatgaag agcaggcggc ggtccgtcag
     3721 gatcagcagg ccatgatcct gcgccgggta ggtcgagcgc aaggtttcct tgataagttc
     3781 cccacagcgc gtcgccctgg aacaataata cgcctgctcg ccgctctcca ggcgcaccgg
     3841 gacgttaatg accggcagca cctccgcctg cgccaggcgc tctgtcttgc gttcctgctt
     3901 ccagttgtgt ttcggcccgg tctgcgggtc tttgccgaat gagagtaatt gaggagtatc
     3961 aacggggcgc gtcacctgct catgtaatgt gagcgtctga ggaataggcg acggaagagg
     4021 ctgccaggtc cgttgggtca gctcagcttg cttgagcgga gctgtttcgc cccgcgaaga
     4081 aaggcgcggc ccggtctttt gaccctcgcc acaccacgat accaggtatt ccgcaaccct
     4141 gcgcgcatca tcgacggcgg caatccctcg cagcgcgacc gtttcacgcc caaaacgcag
     4201 atcaagactt gccgatgcca cgcctaacgt ctgctgcgtt ttcagttgca cctgtgtgag
     4261 accggttaac cggtagcccc tgctcccgac gtagagcatc tccctatata gtcgaaattc
     4321 tttgccccac ccgaacgcac acaccactaa cgcctgttca tcttgcataa caccccaccc
     4381 ccgttactcc acgataaagc tattccatac agttgaaacg agccagcaaa tcagccgtaa
     4441 cgactattga aacatccata tatagtaatt gtagaataca atgccgtatg gatgcgttta
     4501 tggggaaatc caaccctccg taggtgccgg tttaccgcgc acaccgccga ttcatcggcc
     4561 tggatgcctg ccgttgatgc ctattgtggc c
//
LOCUS       DASZAK010000311         8235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_611923, whole genome shotgun sequence.
ACCESSION   DASZAK010000311 DASZAK010000000
VERSION     DASZAK010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8235
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611923"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..2564)
                     /locus_tag="VGT44_05485"
     CDS             complement(96..2564)
                     /locus_tag="VGT44_05485"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV2580285.1"
                     /translation="MIHRQEAVIGGRVMSIETGRVAEQASGAVLVRYGDTVILATVVG
                     SNEPREGIDFFPLTVDVEERMYAAGKIPGGFIKREGRASEHAILACRLTDRPLRPLFP
                     KGYRNDVQIVILVLSADQENDHDIMSIVGASAALCVSDIPFAGPVGAVRVGYIDDQFV
                     INPLESQMSQSRLDLAIAGTSDAVMMVEAGAKELPEELMLEAVRFGQETLQDIIKMQE
                     KLAQAVNMAKRPFQAAPVDEALRAKVAEFVRPRFDAAVNHPDKAARSSALSMVQSDLV
                     AALGAEYPDRLKEIIAFYEKELKAYVRNNILDNGVRPDGRDLKTIRPISCEVGLLPRT
                     HGSAIFTRGQTQVLSVITLGSPGDEQTLDGLGASESKRFMHHYNFPPFSTGESKPMRG
                     PGRREIGHGALAERAVAAVIPAQSEFPYTIRAVSDVLSSNGSTSMGSVCGTTLALMDA
                     GVPIHAPVAGVAMGLITGEGERAGKWAVMSDIQGIEDALGDMDFKVAGTADGVTALQM
                     DIKVKGITYEIMAKALEQAHEGRMYIMEKMLDTIDAPREDLSDYAPRIQAIKINPDKI
                     GAVIGPGGKMIRKIQEESGAKIDIEDDGTVNISATSGEAMQHAMDAVRALTEEVEVGR
                     IYTGTVRRLVDFGAFVEILPGKEGLVRTSQLADYQVNRPEDVVSLGDEITVMVIEVDP
                     QGRVNLSRRAALSGEMPSAAELDNERGGRGGPRGGGMGRPGGGYGGSRPREGSMPNRG
                     YGDRGGYGGGDRGGYGDRGSRPSGGGYGDRGGRPSGGGYGDRGGFSGQRRPMGPGPSG
                     PDRSGPSGPRRDNFGQRSPDRGWQ"
     gene            complement(2940..3209)
                     /gene="rpsO"
                     /locus_tag="VGT44_05490"
     CDS             complement(2940..3209)
                     /gene="rpsO"
                     /locus_tag="VGT44_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV2580286.1"
                     /translation="MPLNAEEKAGIIADARVHETDTGSPEVQVSILTRRINQMTEHMK
                     VHKHDLHSRRGLLMMVGQRRRLLAYLSRKSPERYRALIAKLGLRH"
     gene            complement(3337..3720)
                     /gene="rplL"
                     /locus_tag="VGT44_05495"
     CDS             complement(3337..3720)
                     /gene="rplL"
                     /locus_tag="VGT44_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV2580287.1"
                     /translation="MSVDSIIAEIEQLNVLDLVQLTKTLQEKWGVSAAPVAAAAGPAV
                     AAAEAAPAPVVEEQTEFDAILSEVGPNKINVIKVVRELTGLGLKEAKAVVDEAPKPIK
                     EAVSKEEAQKIADKMAEVGAKVTIK"
     gene            complement(3740..4339)
                     /gene="rplJ"
                     /locus_tag="VGT44_05500"
     CDS             complement(3740..4339)
                     /gene="rplJ"
                     /locus_tag="VGT44_05500"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV2580288.1"
                     /translation="MPTQAKAETIEQLAEKLKRATVAILVQTQGLKVKDMNDLRGKMR
                     AAKVDFQVAKNTLLRIATERNHMTDLDTSIFNGQTAIALGYDDEITAAKAVADYVRTS
                     KVVVLKSGILGGRTLSAQQVEDLAKMPGGKLYAKAQLVGVVQGPLATTYSLLTAPLRD
                     LCYVIQARAEQLQQRSAETTATAEPAETTETAVVETTAE"
     gene            complement(4580..5287)
                     /gene="rplA"
                     /locus_tag="VGT44_05505"
     CDS             complement(4580..5287)
                     /gene="rplA"
                     /locus_tag="VGT44_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV2580289.1"
                     /translation="MASHGKKYLEALKQLEDGKLYSPQEAVSLLKKMNYVKFDPTVEV
                     HMRLGVDPRHADQMVRGVAMLPAGTGKEVKVLVFAQGEKAVEAEAAGADFVGLDDLIK
                     QIEADWTGFDVAIATPDVMGKVGRLGKKLGPRGLMPSPKAGTITMDVARTIQEVKAGR
                     VEFKVDKTALLHIPAGKLSFSDDALMSNLTSIVDAVVRARPSGAKGTYVKSVVLTSTM
                     APGIRLDVPTALALKPA"
     gene            complement(5352..5774)
                     /gene="rplK"
                     /locus_tag="VGT44_05510"
     CDS             complement(5352..5774)
                     /gene="rplK"
                     /locus_tag="VGT44_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015540664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV2580290.1"
                     /translation="MAKRVKAVVKLQIPAGKANPAPPIGTALGPQGINIMQFCKEYNE
                     RTANQVGSIIPAEITIYEDRSFTFITKTPPVADLLKKAVSVEKGSATPNKAKVGSVSR
                     TKVREIAELKMKDLNAIDIEGAERMIEGTARSMGITVD"
     gene            complement(5801..6346)
                     /gene="nusG"
                     /locus_tag="VGT44_05515"
     CDS             complement(5801..6346)
                     /gene="nusG"
                     /locus_tag="VGT44_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV2580291.1"
                     /translation="MQQEQDERRWYAIHTYSGYENKVKSHLEARIASMEMRNKIFRVI
                     VPMEEEVEIKQGQRRTVQRKVFPGYVLVEMIMSDEAWYVVRNTPGVTSFVGSGTRPVP
                     LQDHEIASILKQVTKETEKPKAKISFAKGQSVRVIDGPFTEFIGTVSDINMDRNKVTV
                     LVSFFGRETPVVLDFLQVEKI"
     gene            complement(6435..6803)
                     /gene="secE"
                     /locus_tag="VGT44_05520"
     CDS             complement(6435..6803)
                     /gene="secE"
                     /locus_tag="VGT44_05520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV2580292.1"
                     /translation="MANKMADKKNNASQQEVVEPKARENRSAKAVSREREERRARENK
                     SVKRDSKQVSFWARLRGNRIVNFMFEAYRELRYKVTWPTFEEARNMTIAVIILSSAVA
                     AVLALADLGLFKLFQLISGQ"
     gene            complement(6882..7052)
                     /gene="rpmG"
                     /locus_tag="VGT44_05525"
     CDS             complement(6882..7052)
                     /gene="rpmG"
                     /locus_tag="VGT44_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV2580293.1"
                     /translation="MAAKGKENRIVATLACTVCKHRNYSTSKNKKNNPERMELRKFCS
                     TCRAHTVHRENK"
     gene            complement(7167..>8235)
                     /gene="tuf"
                     /locus_tag="VGT44_05530"
     CDS             complement(7167..>8235)
                     /gene="tuf"
                     /locus_tag="VGT44_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV2580294.1"
                     /translation="FDQIDKAPEERERGITIAIAHVEYETEKRHYAHVDCPGHADYIK
                     NMITGAAQMDGAILVVSAPDGPMPQTREHILLARQVEVPAMVVFLNKVDMMDDEELLE
                     LVELEVRELLAKYQFPGDDVPVIRGSALKALESKGDLSRSDAEAACIWELMDAVDSYI
                     PTPPRATDKPFLMPIEDVFGIKGRGTVGTGRIERGQVKVGDAVEIVGMKEEVRSVIVT
                     GVEMFQKTLDSGIAGDNVGCLLRGVERTDIERGQVLAKPGSIKPHKKFQAQVYVLSKE
                     EGGRHTPFFNGYRPQFYVRTTDVTGAIKLPEGVEMVIPGDNIEMTVELIQPVAMEEGV
                     HFAIREGGRTVGAGVCVKVEN"
BASE COUNT         1502 a         2578 c         2207 g         1948 t
ORIGIN      
        1 cgattcgtcg cgtcctgttg gcctatccga ccgagaacga ggacgcgatg aatcgcgtcc
       61 cgacgaccgg ggccgcattc attaatcgat ctactctatt gccagccgcg atccggggag
      121 cgctggccga aattgtcgcg gcgcggacca ctggggccgc tgcgatcagg gccactgggg
      181 ccaggaccca ttggacgacg ctggccgctg aaaccaccgc gatctccata gcccccgccc
      241 gaggggcgac cgccgcgatc tccatagccc ccgcctgagg gacgactacc gcgatctccg
      301 tagcccccgc ggtctccacc gccatagccc ccgcgatctc cgtagccgcg attgggcatc
      361 gagccttcgc gtgggcgcga gccgccgtag ccgccgccgg ggcgacccat gccgccgccg
      421 cgcggaccgc cgcgaccgcc gcgttcatta tccagctcgg ctgcggaagg catctctccg
      481 ctcaaagcgg cgcgacgcga gagattgacc cgcccctgcg gatcaacctc gatcaccatg
      541 acggtgatct catcgccaag tgaaacgaca tcctccgggc gattgacctg gtaatcggcc
      601 agctgcgatg tgcgcaccag gccctctttg ccgggcaaaa tttcgacgaa cgcgccaaaa
      661 tcgaccagtc gccgcaccgt accggtgtag attcggccaa cctcgacctc ttcggtcagg
      721 gcgcgcaccg catccatggc gtgctgcatg gcctcgccgg aagtggcaga gatgttgacg
      781 gtgccatcat cctcgatatc gatcttcgcg ccgctctcct cctggatctt gcggatcatt
      841 ttgccgcccg gtccgatcac cgcgccaatc ttgtcgggat tgatcttgat ggcctggatg
      901 cgtggggcat agtcggacag gtcttcgcgt ggcgcatcga tggtgtcaag catcttctcc
      961 atgatgtaca tacggccctc gtgcgcttgc tccagcgcct tcgccataat ttcgtaggtg
     1021 atgcccttga ccttgatatc catttgcagc gccgtcacgc catcggcagt accggcaacc
     1081 ttgaagtcca tatcgcccag cgcgtcttcg ataccctgga tatcgctcat gaccgcccac
     1141 ttgcctgccc gctcgccctc gccggtgatg aggcccatcg ccacacccgc gaccggcgcg
     1201 tggatcggca cgccggcatc catcaacgcc agcgtagtgc cacacaccga acccatcgaa
     1261 gtggagccgt tcgacgacag cacatcggag acggcgcgaa tggtataggg gaactcgctc
     1321 tgcgctggaa tgaccgccgc gaccgcgcgt tcggccagcg cgccatgccc tatctcgcgg
     1381 cgaccagggc cgcgcatggg cttgctttct ccggtcgaga agggcgggaa gttgtaatga
     1441 tgcatgaagc gcttgctctc gctcgcgccc aggccatcta gcgtttgctc atcgccggga
     1501 gaaccgagcg tgattacgct cagaacttgc gtctgaccgc gtgtgaagat ggccgagccg
     1561 tgtgtgcgcg gcagcagccc gacctcgcag gagatgggac gaatggtctt cagatcgcga
     1621 ccatccgggc gtaccccgtt atcaaggata ttgttgcgca catacgcctt cagttctttc
     1681 tcgtagaagg cgatgatctc tttgaggcga tccggatatt ccgcgccaag cgccgccacc
     1741 aggtcgctct gcaccatgct cagcgcgctg gagcgagccg ccttgtcggg gtgattaacg
     1801 gcggcatcga agcgaggccg cacgaattct gctactttag cgcgcaacgc ctcgtctacc
     1861 ggtgctgcct ggaaggggcg cttagccata ttgacggcct gcgccagctt ctcctgcatt
     1921 ttgatgatat cttgcagggt ctcctggccg aagcgcaccg cttccagcat cagctcttcg
     1981 ggcagttctt tcgctcctgc ctcgaccatc atcaccgcat cagacgtacc ggcgatggcc
     2041 agatcgagac gcgactggga catctgcgat tcgagcgggt tgatgacgaa ctggtcatcg
     2101 atgtagccca cgcgcaccgc gccgactggc ccggcgaagg gaatatccga gacgcagagc
     2161 gccgccgatg cgccgacgat actcataata tcgtgatcgt tctcctggtc ggcggacagc
     2221 accaaaatga caatttgcac atcgttgcga tatcccttgg ggaagagcgg gcgcagcggg
     2281 cgatcagtca ggcggcaggc gaggatggcg tgttctgaag cgcggccctc gcgcttgata
     2341 aacccaccgg gaatttttcc ggcggcgtac atgcgttctt ccacatcaac ggtcaatggg
     2401 aaaaaatcga ttccctctcg gggttcgttg ctaccaacca cggtagccag aatgacagtg
     2461 tcgccgtagc gcaccaatac cgcgccactg gcttgttctg ccacgcgacc ggtctcgatg
     2521 ctcatgaccc ggccaccgat gacagcttct tgacggtgaa tcatagttcc tccgactacc
     2581 ccctgtggat ttctggccta tgttcctatg ctattagcca ttgctcaatt cgggggttct
     2641 ttacttattc gttacatacc tatcgtaatc gtacacttct caacaactgc gcgtatcttt
     2701 cttcttgcgt ttccctgcgc gctgcctgtt ccttcgcggt ttcaccttgt gcgttgccgg
     2761 ttctctcatg tggggagtgc tttacatgtc tcaattcgcg tctcttcacg cttcctttaa
     2821 cgctctgcgc ctattcattt gtcaggtggg aaatactgga gtcgcggtcg ggtcccaaaa
     2881 aatgtagcgg cgcttgccag aaccgaacgc gccgaccgca tccaacaacc agtgtttgcc
     2941 tagtggcgta agccgagttt cgcgatcaac gcgcgatacc gctcagggct tttccggctg
     3001 agataggcca gcaagcggcg gcgttgacca accatcatca gaaggcccct gcgcgaatgc
     3061 aggtcatgct tgtgtacctt catgtgttcg gtcatttgat tgatgcgcct ggtcaggatc
     3121 gaaacctgca cctccggcga tccggtatct gtttcatgaa cgcgggcgtc agcgatgatt
     3181 cccgcctttt cttcagcatt taacggcact taattacacc tcctctgtgc caggatgagc
     3241 aaagggttaa ctacgcagca ttataccatg ctgcccctgg taatacaacg ttcgctgctc
     3301 aacaccacag tgtggtgttg agcagcgaac gttgtattac tttattgtta cctttgcgcc
     3361 aacctcggcc atcttgtcag cgattttctg ggcttcctct ttagagacag cctctttgat
     3421 cggcttgggc gcctcatcca caaccgcctt ggcctctttc agacccaggc cggtcagttc
     3481 gcgcaccact ttgatgacgt tgatcttgtt gggaccgacc tcggagagaa tcgcgtcaaa
     3541 ctcggtctgc tcctcgacaa caggcgcggg cgctgcttct gcggcggcga cagccgggcc
     3601 tgcggcagcg gcgacgggcg cggcgctgac gccccacttc tcctgaaggg tcttggtcaa
     3661 ctggacaaga tccaggacgt tcaattgttc gatctcggcg ataatgctat ctacggacat
     3721 gattcagtgt tctccttttc tattcagcag tggtctctac caccgctgtt tctgtagtct
     3781 ctgcgggctc cgcagtcgct gtggtctctg cgcttctttg ctgcaattgc tcggcgcgcg
     3841 cctgaatgac gtaacaaaga tcgcgcagtg gcgcggtcaa caagctatag gtcgttgcca
     3901 gcggcccctg gacgacgccg acgagctgcg ccttagcata gagtttcccg cccggcattt
     3961 tggcgaggtc ctcaacctgt tgggcactca gcgttcgacc gccgagaata cctgacttca
     4021 gcacgaccac tttggatgtg cgcacataat cggcaaccgc tttggccgcc gttatttcat
     4081 cgtcatagcc cagcgcaata gcggtctggc cgttgaagat gctggtatcc aggtccgtca
     4141 tgtggttccg ctcagtcgcg atacgcagga gcgtgttttt tgctacctgg aaatcgactt
     4201 tggccgcgcg cattttgccg cgcaggtcgt tcatatcctt gaccttcaag ccctgcgtct
     4261 gcaccagaat ggcaacagtg gcgcgcttca gcttttcggc caattgctca atcgtttctg
     4321 cctttgcctg tgtaggcatg tttcctcctt tcctgaaatg atgcgctaaa tcgctttttt
     4381 ataaaaaatg tggcctcggt agagtaccgg aggccacgta ggattgcttt tcatcatcat
     4441 gcgccggtat cacttcatcg gttgaggcga caccactctc aagacgtact ttcctcggca
     4501 agccgacgcc cgtttgcgcc gattagatgc ctgctggcat acttgctgtc tccggttaat
     4561 ttattctgtt gttaatgctt taggctggtt tcaatgcaag agcggtagga acatccaggc
     4621 gaatgccggg ggccatcgtg gaagtcaata ccacgctctt gacatacgta cccttcgcgc
     4681 cggatggacg ggcgcgcaca accgcgtcaa cgatcgaggt caggttggac atcagcgcat
     4741 cgtcgctgaa tgacaacttc ccggcgggaa tgtgcagcag agcggttttg tccaccttga
     4801 attccacacg gccagctttg acttcctgga tcgtcctggc gacatccatc gtgatcgtgc
     4861 ctgccttcgg actgggcatc aagccgcgcg ggccgagctt cttaccgagg cgtccaactt
     4921 tgcccatcac atcaggcgtt gcgattgcga catcgaagcc cgtccaatcg gcttcgatct
     4981 gctttatcag gtcatcgagt cccacgaaat cggccccggc ggcttctgcc tccaccgcct
     5041 tctcgccctg cgcgaagacc agcaccttga cttctttacc ggtaccggca ggcaacatgg
     5101 ccacgccacg taccatctgg tcagcatggc gcggatcaac gccaagcctc atgtgaacct
     5161 cgaccgtcgg gtcgaacttc acataattca ttttcttgag gagtgaaact gcctcctggg
     5221 gcgaataaag tttgccgtct tcaagctgct tgagcgcctc caggtacttc tttccatgtg
     5281 atgccatgtg ttttgtatgc cctccttgtg gcaagcatta accgcagtga cttgcgggcg
     5341 aatttctctt tctaatcgac ggtgatgccc atgctgcgag cggtgccttc gatcatgcgt
     5401 tccgcgccct cgatatcgat ggcgttgaga tccttcatct tcagctcggc gatctcgcgc
     5461 actttcgtgc ggctgaccga gccgaccttt gctttattgg gggtcgcgga acctttttca
     5521 acgctgactg cctttttcag caaatctgcg actggcggcg tcttggtgat gaaggtgaac
     5581 gagcgatcct cgtaaatggt aatttccgca ggaatgatcg accccacctg gtttgccgtg
     5641 cgctcgttat actccttgca gaattgcata atattgatgc cctgcgggcc aagcgctgtg
     5701 ccaatcgggg gcgcaggatt cgccttacct gctggtatct gtagcttgac tacagcctta
     5761 acacgttttg ccattgttgt ttcaaactcc tcagatcagg ttaaatcttc tctacctgga
     5821 gaaaatccag gacgacgggc gtctcgcggc caaagaacga gaccagcact gtcactttat
     5881 ttcgatccat attaatatcg ctgaccgtgc caataaactc ggtgaacggg ccatcgatga
     5941 cgcgcacact ctggcctttt gcaaagctga tcttggcttt cggcttctca gtttccttcg
     6001 tcacctgctt caaaatcgaa gcgatctcgt gatcctgaag cggaacaggg cgcgtaccag
     6061 aacccacaaa gctcgtgacg ccaggtgtat tgcgcacaac ataccacgcc tcatcgctca
     6121 taatcatctc gaccagcaca tagccgggga agaccttgcg ctgtaccgtt cgccgctggc
     6181 cctgcttgat ctccacctct tcttccatag gcacaatcac cctgaatatc ttattgcgca
     6241 tctccatcga agcaatacgc gcctcaagat ggctctttac cttgttctca tagccggaat
     6301 aggtatggat ggcgtaccat cgacgttcgt cctgctcttg ctgcacgctc gactcccgct
     6361 ctgtgcgtcc cggcgatgat tgcgcggctc cgttggtatg ttctgttacc acagcatttt
     6421 ccctcatcgc gcacttattg tccgctaatc aactggaaga gcttaaacag cccgaggtcc
     6481 gccagcgcca gcactgcagc aacagcgcta gataagatga tgaccgcgat cgtcatattg
     6541 cgagcctcct cgaaagtcgg ccaggtgact ttatagcgca gttcgcgata tgcctcgaac
     6601 atgaaattca ctatgcggtt cccgcgcaag cgcgcccaga atgaaacctg cttgctatcg
     6661 cgctttaccg acttgttctc gcgcgctcgg cgctcttctc gttcgcgtga caccgctttt
     6721 gcgctcctgt tctcgcgagc ttttggctca accacttctt gctggctggc attattcttc
     6781 ttatcggcca ttttgtttgc cacgttggac ctttctcgag taggggcgca attcattgcg
     6841 ccctggtagc gcttaatagg gacgagggct tcattgcgcc cctacttgtt ttcgcgatga
     6901 acagtatgcg ctcgacaggt agaacagaac tttcgcagtt ccatacgctc agggttgttc
     6961 ttcttgttct tcgacgtgga gtagttgcga tgcttgcaaa cggtgcaggc cagcgtcgca
     7021 acaatacggt tttctttccc cttagcagcc atatctctat ctcctcttaa caaacacctc
     7081 cgtagaagcc ggtttaccgt gctcaccgcc gattgatcgg cctgtcccag ccggtcgacc
     7141 agctcctaca gaaatgtcag tatcgcttaa ttttcgacct tgacgcagac gcccgcgccc
     7201 accgtgcgtc cgccctcgcg aatggcgaaa tgcacgccct cttccattgc taccggctgg
     7261 attaactcca ccgtcatctc gatgttgtct cccgggatga ccatctccac cccctccggc
     7321 agcttgatcg ctcccgtcac gtcggtggtg cgcacgtaga actgcgggcg gtagccgttg
     7381 aagaacggcg tgtgccgccc tccctcttcc ttcgataaga cgtagacctg cgcctggaac
     7441 ttcttgtgcg gcttgatcga ccccggcttg gctaagactt gcccccgctc gatgtcggtg
     7501 cgctctaccc cgcgcagcag gcagcccacg ttgtctcccg cgatgccgct gtccagcgtc
     7561 ttctggaaca tctccacgcc cgtgacgatc acgctgcgca cctcttcctt catgcccacg
     7621 atctccaccg cgtcgcccac tttgacctgt ccccgctcga tgcgtcccgt gcccaccgtg
     7681 ccgcgtcctt tgatgccgaa cacgtcttca atcggcatca ggaacggttt gtcggtggcg
     7741 cgcggcggcg tgggaatgta gctgtccacg gcgtccatca actcccagat gcaggccgcc
     7801 tcggcgtcgc tgcgggacag gtcgcctttg ctctccagcg ctttgagcgc cgagccgcga
     7861 atgaccggca cgtcgtcacc gggaaactgg tacttggcta acagttcgcg cacttccagt
     7921 tcgaccagtt ctaagagttc ttcgtcgtcc atcatgtcga ctttgttcag aaagacgacc
     7981 atggccggca cttccacctg gcgcgccagc aggatgtgtt cgcgcgtctg gggcatgggg
     8041 ccatccgggg cgctgaccac caggatggcc ccgtccatct gcgcggctcc ggtgatcatg
     8101 ttcttgatgt agtcggcgtg gccggggcag tccacgtggg cgtagtgccg cttctcggtc
     8161 tcgtactcca cgtgggcgat cgcgatggtg atgccgcgct cgcgctcctc gggcgctttg
     8221 tcgatctggt cgaag
//
LOCUS       DASZAK010000312         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_42812, whole genome shotgun sequence.
ACCESSION   DASZAK010000312 DASZAK010000000
VERSION     DASZAK010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42812"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VGT44_05535"
     CDS             <1..1052
                     /locus_tag="VGT44_05535"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2580295.1"
                     /translation="FRGYCTEGSDDALAEQHVQHTVYYLWLLRQMALFYGCSNDEEAV
                     LYTRNRLESESIIERLLRAANIDTLVLDPAYPLTDECYSPAQMGQLGHCRTVKLLRLE
                     TLMQRLVVEYGDFDECCERYEQAVSDIRAQGYCGFKSIVAYRGGLQVSERTKDEAVAA
                     FDEARMAIRNRALRISQKPLIDYLLFMAFRYAAEQKLPVQFHTGYGDSDTDMRQGNPL
                     HLREVLERGDLRGMKVVLLHESYPYTQLGAYLAAVYPHVYFDLSYAIPFVDRLEMAAF
                     TRQAIGVAPCSKLMFSTDGIHLPEMHWAGAIRGRSVIGQVLGEMVDADEIDEQQGYDL
                     ARMILRETAYAVYNL"
     gene            1139..1657
                     /locus_tag="VGT44_05540"
     CDS             1139..1657
                     /locus_tag="VGT44_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV2580296.1"
                     /translation="MATLAVAMPRVRSERSDAAMSYNSPVDLTLFRVSVWAIIVEGER
                     VLLALRRDIDWWNLPGGGMEAGETVEQAIHREVLEETGLEVEVERITGVYSKPQKNEI
                     ALVMHCRVVKGQAQATEEARECRYFASDDLPSNTLPKHRQRVEDALLNRPEAILRTQN
                     TSTSEDQDLMLE"
     gene            1699..2616
                     /locus_tag="VGT44_05545"
     CDS             1699..2616
                     /locus_tag="VGT44_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2580297.1"
                     /translation="MRQPAMRHSLKMAAPAPVSHDPPALALADLDTLWLQVTGTLCNI
                     ACLHCFITCGPKNHQHEMMTVEQVREALDEARKQGVREYYFTGGEPFLHPDIQQLITM
                     TLAQGPLSILTNGILIDEEMATWLANTFRSSQYSLDLRVSLDGSTPEDNDRVRGKGTF
                     ARITAGIERLARHGLNPVITVTEVRPDMGMPKARTAFIEFVRSLGLEKPRMKYLTPFL
                     IGREEQRTRGYTPDELLHEGDLLPGEAEKLQCSSCRMITDDGVYPCPLVINIPSAKMG
                     ERFSDGLRPIKLAWQPCYTCHIYGVTCRT"
     gene            2632..3423
                     /locus_tag="VGT44_05550"
     CDS             2632..3423
                     /locus_tag="VGT44_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2580298.1"
                     /translation="MSYSIAVFGGCYNNSLSLQATIDDAHRRGARAIYCLGDMGGFGP
                     HPDRVFPILWDNEVICMQGNYDHSIGNRLADCACGYTDPRDNYYAQISYDYTLAHTSD
                     RYKDWLRELPPRIRLEVAGQRLLMAHGSPRRVNEFLWESTTPEPFLARLFDDYEADII
                     LCTHTGIQWMRELAGGRRLVNVGAIGRPPNDGQTTVWYALLSIDPERNHIDTELIPVA
                     YDYERLIYDMRQELLPEEFIETIRTGWWTTCLEILPAKERARGRF"
     gene            complement(3420..>3710)
                     /locus_tag="VGT44_05555"
     CDS             complement(3420..>3710)
                     /locus_tag="VGT44_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580299.1"
                     /translation="AHFALPFAGKANFDVVFERVKQALRPGGIFAGQVFGVNDEWNTP
                     GQDLAFHTRAQAEELLRGFELIEFSEEDVDSHTADGTPKHWHVFNIIARKPS"
BASE COUNT          750 a         1104 c         1140 g          716 t
ORIGIN      
        1 gctttcgcgg gtattgcacg gaggggagcg atgatgcgct ggcggagcag catgtgcagc
       61 atacggtgta ctatctctgg ctgctgcggc aaatggcgct gttctatggc tgctccaacg
      121 acgaggaggc ggtcttgtat acgcgcaacc gcctggagag cgagagcatt atcgagcggc
      181 tgctgcgcgc ggcaaatatc gatacgctgg tgctggaccc ggcctatccc ctgaccgacg
      241 agtgctattc gcccgcgcag atggggcaac ttggccactg ccggacggtg aaattgctgc
      301 ggctggaaac cttgatgcag aggctggttg tcgagtatgg cgacttcgat gaatgttgcg
      361 agcgttacga gcaggccgtc agcgatattc gcgcgcaggg ctactgcggc tttaagagca
      421 tcgtggccta tcgcggcggg ctgcaagtga gcgagcgcac aaaggacgag gctgtggcgg
      481 ccttcgacga ggcacgaatg gctatacgaa atcgagcgct gcgcatcagc cagaaacccc
      541 tgattgatta cctcctgttt atggctttcc gttacgccgc cgagcagaaa ttgccggttc
      601 agttccatac gggctacggc gacagcgata ccgacatgcg ccagggcaac ccgctgcacc
      661 tgcgcgaagt gctggaacgc ggcgatctgc gcggcatgaa ggtggtgctg ctgcacgaaa
      721 gctaccccta cacgcagctt ggcgcgtacc tggccgccgt ctacccgcac gtctatttcg
      781 atctgtccta cgctatcccc tttgtggatc gcctggaaat ggcggccttc acacgccagg
      841 ctatcggcgt cgcgccgtgc agcaagctga tgttcagtac ggacggcatc cacctgcccg
      901 aaatgcactg ggcgggcgcg atacgcgggc gcagcgtgat cgggcaggta ttgggcgaaa
      961 tggttgatgc cgacgaaatc gacgaacagc agggctatga cctggcccgc atgattttac
     1021 gcgagacggc gtatgcagtc tataatttat aaagcagata attttgtagc atggcgaccg
     1081 caagggtcgc cactacaata tcctaaatcc aattggtcag ttaagcttcg ttatcgtagt
     1141 ggcgaccctt gcggtcgcca tgccgcgcgt gcgcagcgaa aggtccgatg ccgccatgtc
     1201 ctataattcg cctgttgatc ttacgctctt tcgcgtcagc gtctgggcga ttattgtaga
     1261 gggcgagcgc gtcttgctgg cgctgcgccg tgatatcgac tggtggaacc tgcccggagg
     1321 cggcatggag gcgggcgaaa ccgttgagca ggccattcac cgcgaagtac ttgaagaaac
     1381 cgggcttgag gttgaagttg agcgcatcac aggcgtctat tccaagccgc agaagaacga
     1441 gattgcgctt gtgatgcatt gccgcgttgt aaaggggcaa gcgcaagcaa ccgaggaggc
     1501 gcgtgagtgc cgttactttg cttcggacga tctgccctcc aacacgcttc ccaagcaccg
     1561 ccagcgcgtc gaggatgcgc tgctcaaccg gccagaggcc atcctgcgca cgcagaatac
     1621 ttccacgagt gaagaccagg atctcatgct tgagtaaggg tcttgaaacg gattgtggta
     1681 ataggatagg aggtcacaat gagacaaccg gctatgcgtc attcgttgaa gatggccgcg
     1741 cccgcgcctg tcagccatga tccgccagcg ctggcgctgg ccgacctgga tacgctctgg
     1801 ctgcaggtga cgggcacgct ctgcaatatc gcctgcctgc actgcttcat cacctgcggg
     1861 ccgaagaacc accagcacga gatgatgacg gtggagcagg tgcgcgaagc gctggacgag
     1921 gcgcgaaagc agggagtgcg cgaatactat tttacgggcg gggaaccgtt cctgcatccc
     1981 gacatccagc aacttattac gatgacgctg gcgcagggac cgctcagcat ccttaccaac
     2041 ggcatattga tcgatgagga gatggccacc tggctcgcca acacgttccg ctcgtcgcag
     2101 tattcgctcg accttcgtgt gagcctggat gggagtacgc cggaggacaa cgacagggtg
     2161 cgcggcaagg gtaccttcgc gcgcattacg gctggcatcg agcggctggc gcggcatggc
     2221 ctgaacccgg taatcaccgt cacggaggtg cgccccgata tggggatgcc gaaggcgcgc
     2281 acagccttca tcgagttcgt gcgcagcctg ggacttgaga agccgcgcat gaagtacctg
     2341 acgcccttcc tgatcggtcg cgaagagcag cgcacgcgcg gctatacccc cgacgagctg
     2401 ctgcacgagg gcgacctgct gccaggcgag gccgaaaagc tgcaatgcag cagttgtcgc
     2461 atgattaccg acgacggcgt ctatccctgt ccgctggtca tcaatattcc atccgcgaag
     2521 atgggcgagc gcttctcgga tgggctgcgg ccaatcaagc tggcctggca gccgtgctat
     2581 acctgccaca tctacggcgt cacctgccgc acctgatcgg gaggttcatc tatgagctat
     2641 agcatcgccg tttttggcgg ctgttacaat aattccctgt cgttgcaggc caccatcgac
     2701 gacgcccacc ggcggggcgc gcgagctatc tattgcctgg gtgacatggg cggcttcgga
     2761 ccccatcctg atcgcgtctt cccgatcctg tgggacaacg aggtgatctg tatgcagggc
     2821 aactacgacc acagcatcgg caaccggctg gcggattgcg cctgcggtta caccgatccg
     2881 cgcgacaact actacgcgca gatcagctac gactatacgc tcgcgcacac ctccgaccgc
     2941 tacaaagatt ggctgcgcga gctgccgccg cgcatacgcc tggaggtcgc cggacagcgt
     3001 ctcctgatgg cgcatggctc gccgcgccgc gtcaatgaat ttctctggga atccaccacg
     3061 ccggagccgt ttctggccag gctcttcgac gattatgaag ccgacatcat cctctgcacg
     3121 cacaccggaa tccagtggat gcgcgaactg gccggtgggc gtcgcctcgt caacgtcggc
     3181 gcgataggcc gcccgcccaa cgacgggcag acaacggtgt ggtacgcgct gctgagcatc
     3241 gacccggaac gcaaccacat cgataccgaa ctgatccccg tcgcctacga ctacgagcgc
     3301 ttgatatacg acatgcgcca ggaactcctg ccagaggagt ttattgaaac cattcggaca
     3361 ggatggtgga ccacctgcct ggagatcctg ccggcgaagg agcgcgccag gggacgattc
     3421 taggacggtt tgcgcgcaat gatattgaag acgtgccagt gtttcggcgt gccatcggcg
     3481 gtgtggctat ctacgtcctc ttcactgaat tcgatcagtt cgaacccgcg cagcagttct
     3541 tcggcctgcg cccgcgtgtg aaaggccagg tcctgccccg gcgtattcca ttcatcgttc
     3601 acaccgaaga cctgtccggc aaaaatgccg cccggtcgca gcgcctgctt gacgcgctcg
     3661 aacaccacgt caaagttcgc cttccccgcg aatggcagcg cgaaatgcgc
//
LOCUS       DASZAK010000313         5780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_434393, whole genome shotgun sequence.
ACCESSION   DASZAK010000313 DASZAK010000000
VERSION     DASZAK010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434393"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1758)
                     /locus_tag="VGT44_05560"
     CDS             complement(49..1758)
                     /locus_tag="VGT44_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580300.1"
                     /translation="MQRNGKWFVFLMLTLAILLASTFAASFVFARGTGSPASAGADAS
                     AQAATAPLVGMHTITMSNIPAATRSSSGSRAEGDSMYRGVDPAVYAQRKAAAAHNPNA
                     PLNAHPVQAVRAANLPAVINQFNGLADSSANCPIFGSCRHPDMALAASSSWVLQGVNE
                     AWSVYDTSGTVQSGWPKNYQQFFGVPNPPNHCDTLPYMVDVRAFYDPADGRFWAAMLQ
                     DEGAYGYNNCPFQALYWIAVSATSNPTGAWHIYTFDMAQGTTFPVDFTQLGFNGSAVY
                     FSGNLLNLSGSTFEYAEVFAASKSLMESGSTVTAKGFKKLKASNTLVDSVQPVETQAL
                     GSSAPTAELLVSSFNVKSGGGSCSKGCSGVVAWAFANALKTPSLSKVVVSTPTYTLPP
                     YADQPGCSHCIETFDTRIGATPVYNNGKISFALESGVNNGTQVVPGVFWGQITPTLSS
                     AGKITGATLFQSGIFSFTGDQAASYGAVMDDSQDSVFLAFATMSHTIKPGDMYTVHLT
                     TDTKGTMETPAYLIQGQAAKTTQYYGDYQATSYDGSSGNHTWFATEYVNAGGDWSTFI
                     ADV"
     gene            2011..3564
                     /locus_tag="VGT44_05565"
     CDS             2011..3564
                     /locus_tag="VGT44_05565"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2580301.1"
                     /translation="MSGSSSSARYLQFAKNVAHVPVDRAWTNGQRFGNFAISVSCCEQ
                     AQHFSFSASKLALDQAGAYIEETSCGLPGYLELYRTHRTHLLQRRSAGPSAHPEPVAV
                     TWRLAFLKVEQANSAAADLLRLCAFLAPDAIPEAMITEGGTKLGPILGPVTTDAFILN
                     AAIEVLRKYSLVRRNPDTKTLIIHRLVQAVLKESMDEQSQRQWAVRTVCAMNDAFPEG
                     YFESWSRCQEYLPHVLACAQLIAQYGLMLAEAALLLQRAGAHTHERAQYRNAETLLQQ
                     SIPICQQVLGFENSQTVQSLNYLAETYYEQGKYEQAEPLFQQALAINEQLFGSEHSHT
                     ASSVNNLAVLYRTQGKYEQAEPLFQQALAIREQVLGSEHQETAESFNNLAYFYHHQGK
                     YEQAESLYQRALLIAEQIRGPEHPFTALVLHNLARLYHDQGKYVEAESLYQRALSIRE
                     RMLGLEHPRLAITQAFLGRLYRDQGRYAEAELLFQQALTVQEKMLGPEHPDTAATRKD
                     FLELRENQT"
     gene            3708..5051
                     /locus_tag="VGT44_05570"
     CDS             3708..5051
                     /locus_tag="VGT44_05570"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV2580302.1"
                     /translation="MNLVSFSIKTKGIRNFSRRLWTVFTRFGFSEARISRALHTLVET
                     LGKYDAAPTFFIPAVVLQRHVALIHEITGAGAEMGVHGYIHNDYRYLSEVGQYKQMEQ
                     AVAVFESTHTPFNGFRNPYLGWTEDTLRVLTALGFAYESNEAVLHPVIDLDLLTPSLR
                     SGYEKSFELFQALACNAYVLRPHVEESLLRIPTSIPDDEMLFDRLRITDAQEVGRIWR
                     KIMERVYDLGGIYTLNLHPERALLCRRALDILLSFASSQALPVWLPRLNEVAQWHQER
                     RQFKLEIVEESPRRWRFAATCSSRATLLARHLTLEDQAASPWFGPDVRVLEQSGVVSA
                     DRCPCIALSAQTPQEVLEFLQEQGYPALRCAPEEGQRYALYLDLPGGLGDTREEQRAR
                     RGSLLEQLVQLEAPLLYFGYWPDGCRAALAVSGDIDSITAQDFFLRVFEVSKHAD"
     gene            5041..>5780
                     /locus_tag="VGT44_05575"
     CDS             5041..>5780
                     /locus_tag="VGT44_05575"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2580303.1"
                     /translation="MPTDNTSLLAHVPCSASIERQLPDNFYYQQPWLDLLADVYGYTI
                     IPLTITDQNGQVSGFLPLCSIHSPITGRRLVSLPFSDYCPMLAADEASANALADQAVE
                     LARQQKAKYLELRTGGCKALSARADLVEEALYVRWLISLAADPDAIWSGLRKPIQRQV
                     KKSQKLGVQVRVARDRQDVAHYYRMHLLTRSKKHGMPAQPQRYFYRLWDAFASSGSMQ
                     LLLAEYEGAVIAGMVLLASGSTIRYAYGA"
BASE COUNT         1209 a         1637 c         1643 g         1291 t
ORIGIN      
        1 attcattgcg cccctacatt tcgtttgaac cttgacgaac tattagtttt acacgtcggc
       61 gatgaacgtt gaccagtcac cgccggcgtt aacgtattcg gtcgcaaacc aggtatgatt
      121 accgctgctg ccgtcatacg aagtcgcctg gtaatcgccg taatattgcg ttgttttggc
      181 cgcctgcccc tggataaggt acgcaggcgt ctccatcgtt cccttcgtgt cggtggtgag
      241 gtggacggtg tacatatcgc cgggttttat ggtgtgactc atcgtggcga atgccaggaa
      301 gacgctatcc tgcgagtcgt ccatcaccgc accataggag gccgcctggt cgccggtgaa
      361 ggaaaagatg ccgctctgga aaagggtcgc gccggtgatc ttccctgctg aactcagcgt
      421 cggggtgatc tgtccccaga agacgcctgg aacaacctgg gttccgttgt tgacgccgct
      481 ttcaagcgcg aacgagattt tcccattgtt gtagacagga gtcgcgccga tgcgtgtgtc
      541 gaatgtttcg atacagtgtg agcagcctgg ttgatcggca tagggcggca gagtatacgt
      601 tggggtcgat acgaccactt tggagaggct gggcgttttc agcgcattag cgaaggccca
      661 ggcgacgacg ccgctgcagc ctttggagca agagccgcca cccgacttga cgttgaacga
      721 gctgaccagc agttctgcgg ttggggcgga gcttccaagc gcctgtgttt cgaccggctg
      781 gaccgaatcc acgagtgtat tgctggcctt caatttctta aagccctttg ctgtgacggt
      841 cgaaccactc tccatcaagg atttgcttgc cgcgaacact tcggcgtatt cgaaggtgga
      901 accgctcagg ttgagcagat tgcctgagaa atagacggcg ctgccattaa agcccaactg
      961 cgtaaaatcg accgggaagg tggttccttg cgccatatcg aaggtgtaga tgtgccacgc
     1021 gccggtcggg ttactggtcg cgcttacggc aatccagtac agcgcctgga agggacaatt
     1081 gttgtagcca tatgcgcctt cgtcctggag catagctgcc cagaaacgtc cgtcagccgg
     1141 atcgtaaaat gcccgcacat ccaccatgta tggcagcgtg tcgcagtgat tcggcggatt
     1201 cggcacgccg aagaattgtt ggtagttctt cggccaaccg ctttgcacag tgccagatgt
     1261 gtcgtaaacc gaccacgctt cgttgacgcc ttgcagcacc cagctcgaag aagctgccag
     1321 cgccatgtcg ggatgcctgc aactgccgaa gatagggcag ttcgcggacg aatcggccag
     1381 cccgttgaat tgattgataa ctgcggggag gtttgctgct ctgacggcct gaacgggatg
     1441 ggcgttcaac ggggcattcg gattgtgcgc cgccgctgct ttgcgttgag cgtatacggc
     1501 tgggtcgaca cccctgtaca tcgagtctcc ctccgccctg ctgccggacg aagaccttgt
     1561 cgcggctggt atattgctca ttgtgatggt atgcatcccg accagtggcg cggtcgccgc
     1621 ctgggccgag gcatcagcgc cagcgctggc ggggctgcct gtccccctgg caaacacaaa
     1681 actagcggcg aaggtactgg ccaggagtat tgcgagggtt agcatgagga aaacgaacca
     1741 tttaccattt ctttgcatta cgaaacctcc tcaagaaaga cgcgctactg aacaaccgag
     1801 aagctaattg tcgcaattgt aagcgctttg cacttctatt tggatattga acaggttaag
     1861 aaatggttaa cggaaagcgg tgctggtgtt gcggaggttt gggtttgtct atggttggac
     1921 atccgtccag gtcgagatgt gttttgcgag ctttgacagt gttgctcatc ctgggccttg
     1981 aagcgatcaa attaagccga tgcgaaccgc atgagcggca gcagcagctc ggctcggtac
     2041 ctccaatttg ccaaaaatgt tgcacatgtg ccggttgacc gtgcgtggac taatggtcag
     2101 cgattcggca attttgcgat cagtgtgtcc tgctgcgagc aggctcagca cttctcgttc
     2161 tcggcgagta agctggccct tgaccaggcg ggtgcctaca tcgaggaaac gagttgtggc
     2221 ctccctggtt acctggagct ttaccgcacc caccgcaccc atcttctgca acgtcgcagc
     2281 gctggtccat cagctcatcc cgaaccggta gcggtcacct ggcgactggc ctttctcaaa
     2341 gtggagcagg ccaactctgc tgctgccgat ctgttgcgcc tttgtgcttt cctcgctccc
     2401 gacgccattc ccgaggctat gatcaccgaa gggggcacca aacttgggcc tatccttggc
     2461 cccgttacta ccgatgcctt catcctcaac gccgccatcg aagtccttcg caagtactcg
     2521 ctggtgcgac gcaatcccga caccaaaaca ctgatcatcc atcgcctggt acaagcagtc
     2581 ctcaaagaga gcatggacga acagtcgcaa cgacaatggg cagtgcgaac ggtatgcgca
     2641 atgaatgatg ccttcccaga agggtatttt gaaagctgga gtcgctgtca ggagtatctg
     2701 ccccatgtgc tggcctgtgc tcagcttatc gcacagtatg gtctcatgct cgctgaagcg
     2761 gcgctcctgc tgcaaagagc tggagcgcat acgcacgagc gggcccaata tagaaacgcg
     2821 gaaacactgt tgcagcagag cattcctatc tgccagcagg tactggggtt cgaaaattcc
     2881 cagacggtcc agagtctgaa ttacctggca gaaacctact atgagcaggg caagtacgaa
     2941 caggcagaac ctctcttcca acaagctctg gctatcaatg aacagctatt tggatcagag
     3001 cactcccaca cagcttctag cgtcaataac ctggcagtac tctatcgaac ccaggggaag
     3061 tacgaacagg cagaacccct cttccaacaa gctctggcga tccgcgagca ggtgcttgga
     3121 tcagagcatc aggagacagc agaaagcttc aataatttgg catactttta ccaccatcag
     3181 gggaagtacg agcaggcaga atcgctctac cagcgcgctc tactgattgc ggaacagatt
     3241 cgaggaccag agcacccttt tactgcactg gttcttcaca acctggctcg cctgtatcat
     3301 gaccagggca agtatgttga ggcagaatcg ctctaccagc gcgctctgtc catccgtgag
     3361 cggatgctgg ggctggaaca tcctcgcctt gccataactc aggcattcct gggccgtctc
     3421 tatcgtgatc agggcaggta tgctgaggca gaacttctct tccagcaggc tctgacagtc
     3481 caggagaaaa tgctgggtcc tgagcaccct gatacggcag cgacccgcaa ggacttcctc
     3541 gaactgcgag agaatcaaac ttaagggatg gggggaagcg acgtagattt ctcaatagcc
     3601 agcgccgcgc ctgcaccaat taaatgtaag gtgaaacacc aaaaagatac ccggcccatg
     3661 ctatgctttt tcaggcatgg atagcgcagg gaggtttcac agagtacatg aaccttgtct
     3721 ccttttcgat taaaaccaag ggcatccgta atttttctcg ccgcctctgg accgttttta
     3781 cccgcttcgg tttctccgag gcgcgcataa gccgggcgct acatacgctg gtagagacgc
     3841 ttggcaagta tgacgcagcg ccgacatttt ttataccggc ggtggtgcta cagcgtcatg
     3901 tggcgctgat tcacgagatt accggcgcgg gcgctgaaat gggtgtgcac ggctacatcc
     3961 acaacgatta tcgctatttg agcgaggttg gtcaatataa gcaaatggag caggcggtcg
     4021 cggtattcga aagcacacac acccctttca atggcttccg caatccctat cttggctgga
     4081 ccgaggatac actgcgcgtc ctgaccgcgc tcggttttgc ctatgagagc aatgaggctg
     4141 tcctgcaccc ggtcatcgac ctggatcttc tcacgccctc gctgcgaagc gggtacgaaa
     4201 aatcatttga actctttcaa gcgcttgcct gtaacgccta tgtcctgcga ccgcatgtcg
     4261 aagagtctct gctgcgcatt ccgaccagta ttcctgatga cgagatgttg tttgaccggc
     4321 tgcgtattac tgatgcgcag gaggttgggc gtatctggcg caagatcatg gagcgcgtct
     4381 acgatctcgg aggcatctac acgctcaacc tgcacccgga acgcgccctg ctttgccggc
     4441 gcgcgctgga cattctgctc tccttcgcca gcagtcaggc gctgccggtc tggctgcctc
     4501 gtctgaatga ggtcgcccaa tggcatcagg agcgccgtca gttcaagctg gaaattgtcg
     4561 aggagtcgcc gcgccgttgg cgctttgcag ccacatgcag ttctcgcgcg acattgcttg
     4621 cccggcacct tacgctggaa gatcaagccg ccagcccctg gtttggcccg gatgtgcgtg
     4681 tattggagca gagcggtgtc gtcagcgccg accgctgtcc ctgtatcgcg ctctcagcgc
     4741 aaacgcctca agaggtgctg gaattcctcc aggaacaggg gtatcccgcg ctgcgctgtg
     4801 cgccggagga aggccagcgg tacgcgctct atctcgatct gcccggcggc ctgggagaca
     4861 cgcgcgaaga gcagcgagcg cggcgcggca gcttactcga gcagctcgtt caattagaag
     4921 cgcccttgct ctattttgga tactggcctg atgggtgtcg cgcagcgctt gctgttagcg
     4981 gggatattga ttctatcaca gcgcaggatt tctttttgcg cgtatttgag gtctcgaaac
     5041 atgccgactg ataacacatc cctgctcgct cacgtcccat gtagcgccag catagagcgg
     5101 caacttcctg ataatttcta ctaccagcag ccctggcttg atctactcgc cgatgtgtat
     5161 ggttacacca tcataccgct cacgatcacc gatcaaaacg gccaggtctc cggcttcctg
     5221 ccgctctgct ccatacatag cccgattacg gggcgacgcc tggtgtccct gccattttcc
     5281 gattattgtc ccatgctcgc cgccgacgaa gccagcgcga acgcgctggc cgaccaggca
     5341 gttgagcttg cccggcagca aaaggcgaag tacctggagc tgcgcaccgg cggttgcaaa
     5401 gcgctctccg ctcgagctga tctggtggag gaagcgttgt atgtgcgctg gttgatttcg
     5461 cttgccgctg atccagacgc gatctggtca gggttgcgca agccgatcca gcgccaggtg
     5521 aagaaatcgc agaagctcgg cgtgcaggtg cgcgtggcgc gagaccgtca ggatgtcgct
     5581 cactactacc ggatgcacct gctaacgcgc agcaaaaaac atgggatgcc cgcgcagccg
     5641 cagcgctatt tctatcgttt atgggacgcc tttgcttcca gcggttcaat gcagctcttg
     5701 ctggcggaat acgagggcgc ggtgatcgcc ggcatggtgc tgctcgcctc cggctcgacc
     5761 atacgctatg cctacggcgc
//
LOCUS       DASZAK010000314         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_79105, whole genome shotgun sequence.
ACCESSION   DASZAK010000314 DASZAK010000000
VERSION     DASZAK010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79105"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..2176)
                     /locus_tag="VGT44_05580"
     CDS             complement(59..2176)
                     /locus_tag="VGT44_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEV2580304.1"
                     /translation="MRQKHHLRLLLGGLAGLGVLLMWNWLPVFSQSPVALAHGFVIGS
                     DPVDGSTIPGAPAVVRIFFNADISTASRVQVFVFVPGGSPGGSEIDRGISVVPAGHAM
                     ELDTPLLSPATLPQGSYEVRWTAVASDDGHATHGLIGFNVGVSATGLTGTTIIGPSTS
                     NILPQMDLPGVLATLWEWVTLAALTFWIGLVAMEAIFVFGVMPSTHGAADNAIRLLRK
                     QGRPLQWLCLYALLAGEIINLVLRGMALSEFQQSGSIDLASLGAVLLQTGYGYLWLAR
                     LGLIACALVFGWWTTRAVKKTHISSRGRAMRAIRGQVKGEAAQRLAPTSSTAASSRYR
                     QLRLQIEDELTQEHEPHTDGYATFATEMTPEDATVVSRRHSITWLLLASLIALTIAYS
                     SDTAQLAQTHITAIALNWLYLLAQSAWLGGVAYLGFVLLPLLPTIEPETHGTLLVNVL
                     RYYVPLLLAALGVLLLNGLFSIETTINSPDQLINDPYGRTLLIAQLFFTLLVIFSAYS
                     FFYLLPATHRQMVLLPVVNADLPARRARRSALEQSISSLKRAMHILSLLGAGILLCVA
                     LMAFYAPPIVFPPATSGTGSAQGTSTAQSIQTKTVGTLVISLQVSPARADTTNFVTVI
                     LKDTDGSSVTNARVQLRTNMQIMDMGTATKAAQGQAGNSSYTASFTPDEAFSMLGTWV
                     ISLTIQQTGQPAAQTQFFVTLLE"
     gene            2345..3472
                     /locus_tag="VGT44_05585"
     CDS             2345..3472
                     /locus_tag="VGT44_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580305.1"
                     /translation="MVTKRIQTLLPCLALAALLAGLSSCGWQPASSQPGAKNEPDRVQ
                     IAIHALDLQAGKPVVTLTNVALVRQLYATLLALSPLPQNLACTADFGPSYTLTFLQGE
                     KTLTTANAQRYGCRRVSLAGEQQDRGATQEFWAQLDQAIFQATPVARPQQLAIQRTLQ
                     LDQPPQTAQITSIETVQRLYNAILALPSFPSNGACSPASLPEYQLVFHTPDQAIPSVL
                     DETCHTISLEGNYQSRSGTFVMTDQFTRLFEQTLATASFAPARPDRLTLDVEPARGVA
                     RQITIADAGLRQQLYAKLFALPVGKAQPDCPPVEDKVAGKGQWYVLTFTQWGLPVLVD
                     VDAYEGSCLFLSLDAGQGMAGGQVLQWDAAFWNLVHQAANS"
BASE COUNT          785 a         1126 c         1085 g          722 t
ORIGIN      
        1 ggtgtgaaca gcaagccatc aggccgatga atcggcggtg tgcgcgataa atcggcacct
       61 actcaagcag cgtcacgaag aattgcgtct gcgccgccgg ttggcctgtc tgctggatag
      121 tgaggctgat aacccacgtg ccgagcatcg aaaatgcctc atccggggta aagctggccg
      181 tatagctcga attgcccgcc tggccttgcg cggctttggt agcggtgccc atgtccatga
      241 tctgcatatt ggtgcgtaac tgcacgcgcg cgttggtgac ggagctgccg tcggtgtcct
      301 tcaagatcac ggtcacgaag ttggtggtgt cggctcgtgc gggcgagacc tgtagcgaaa
      361 tcaccagggt gccgaccgtt ttcgtctgga tactttgggc cgtcgacgta ccctgcgcgc
      421 ttcctgttcc gcttgtcgcg ggcggaaaga caatcggcgg cgcgtagaaa gccatcaggg
      481 cgacgcagag caggattcca gcgcccaaca atgagagaat gtgcatggcg cgtttcaggc
      541 tgctgatcga ttgttcgagt gcagatcggc gcgcgcgtcg ggctggcaga tcggcattca
      601 cgacgggcaa cagcaccatc tgcctgtgtg tggcgggcag caagtagaag aagctataag
      661 cgctgaagat taccagcagg gtaaagaaca gctgcgcgat cagcaaggta cgcccatagg
      721 ggtcattgat caattgatcc gggctgttga tcgtggtctc gatgctgaac agcccgttga
      781 ggagcagtac gcccagggca gccagcagca ggggaacgta gtagcgcagc acgttgacga
      841 gcagcgtacc atgcgtctcc ggctcaatcg tcggcagcag cggcagcaac acaaacccca
      901 ggtaagcgac gccgcccagc caggcagact gggcgagcag gtacaaccag tttagcgcaa
      961 tcgcggtgat gtgcgtttgc gccagctgcg ccgtgtcact agaataggcg atcgtcagcg
     1021 caatcagcga tgccagcagc agccaggtga tagaatggcg gcgcgagact accgttgcgt
     1081 cttccggggt catctcggtc gcgaacgtag cgtagccatc agtatgcggc tcatgctcct
     1141 gtgtcagctc atcctcgatc tgcaagcgca attggcgata gcgactgctt gccgccgtgg
     1201 acgacgtagg cgcgagacgc tgcgcagctt ctcccttgac ctgcccgcgt atggcgcgca
     1261 tcgcgcgtcc ccggcttgat atgtgtgtct ttttcactgc gcgcgtcgtc caccagccaa
     1321 agaccagcgc gcaggcgatc aagcccaggc gcgcgagcca gagataaccg tagccggtct
     1381 gcaagagtac agcgccaagg cttgccaggt cgatgctacc gctctgctgg aattctgaca
     1441 gggccatgcc gcgcaagacc aggttaatga tctctccggc aagcagcgcg tataggcaca
     1501 gccattgcag gggccgcccc tgtttgcgca gcaggcgtat agcattgtcg gctgcgccat
     1561 gcgtggaggg catcacgccg aagacgaaga tggcctccat cgccaccagc ccgatccaga
     1621 aggtcagcgc cgccagcgtc acccactccc acagcgtcgc caggacgcct ggcaggtcca
     1681 tctgcggcaa aatattgctg gtactcggcc cgattatcgt cgtacccgta aggccagtcg
     1741 ccgagacgcc cacgttaaag ccgatcagcc catgcgtggc atgtccatca tcgctggcga
     1801 ccgccgtcca gcgcacttca tagctgccct gcggcagcgt ggcgggcgaa agcagcggcg
     1861 tatcaagctc catcgcatga ccggcaggca cgacgcttat acctctatct atctcgctcc
     1921 caccaggtga gccgcctgga acgaagacga atacctgcac gcggctggcc gtgctgatat
     1981 ccgcgttgaa gaagatgcgc acaacagccg gcgcgcccgg gatcgttgac ccatccaccg
     2041 ggtcagaacc aatcacgaag ccatgcgcca gcgccaccgg tgactgtgaa aacaccggca
     2101 gccagttcca catcagcaac actccgagac ccgccagccc ccccagcaaa agcctcagat
     2161 gatgtttttg tcgcatcgtt cgcctcagcg cattgttttg catgacctga attgtacatc
     2221 tgtagcgatg tgcgcgcaag aggcacagca agctcacaca aaggggaacg aatttgctcg
     2281 tatcaacgtc tctcaaagag gaagcatgct gtattctctt agatggtttg caaggagatt
     2341 cctcatggtc acaaagcgta tacaaacgtt gctgccctgc ctggcgctag cagcgttact
     2401 ggccggactg agtagctgcg gctggcaacc ggcctcgtcc cagccgggcg cgaagaatga
     2461 gccggaccgg gtgcaaatcg ccattcacgc cctcgatctt caggcgggca aaccagtcgt
     2521 gaccctgacg aacgttgcgc tagtgcggca gctctacgca acgcttcttg cgctgtcgcc
     2581 attgccgcaa aacctggcct gcacagcgga tttcgggccg agttataccc tgaccttcct
     2641 gcagggagag aaaacgctga caaccgctaa tgcgcagcgc tatgggtgca ggcgtgtctc
     2701 tctggcgggc gaacagcagg acagaggggc aacccaggag ttctgggcgc aactggacca
     2761 ggccatcttt caggcgacgc cagttgccag gccgcaacag ctcgcaatcc agcggacgtt
     2821 gcagctcgat caaccgcctc agaccgcgca gatcacctcg attgagacgg tgcagcgcct
     2881 gtacaatgct atcctggcgc ttccgtcgtt tccctcgaat ggagcctgct cccctgcgtc
     2941 actgcccgaa taccagcttg ttttccatac gccagatcaa gccattccct ccgttcttga
     3001 cgaaacttgc cacacgattt cgcttgaagg caactaccaa tctcgcagtg gaactttcgt
     3061 catgactgat caattcacgc gcctcttcga gcagacgctg gccacagcct ccttcgcccc
     3121 tgcgcgcccc gatcggctca cgctggacgt tgagccggca agaggcgtcg cccggcagat
     3181 cacgattgcc gacgcgggat tgaggcagca gttgtacgcg aagctttttg cgctgccggt
     3241 ggggaaggca cagcccgatt gtccaccagt agaggataag gtggccggga aagggcagtg
     3301 gtacgtcctt acctttacgc agtggggtct gccggtcctt gtcgatgtcg atgcctacga
     3361 gggaagttgc ctcttcctct ctctcgatgc tggccagggc atggccggag gccaggtgtt
     3421 gcaatgggat gcagcgttct ggaatcttgt tcatcaagcc gcgaattcgt agccggcctt
     3481 cttgtatagt caccggggca tttctatgct atgatggccc taccgttcat caagtaagaa
     3541 tctatggaga gggaggtagc ctgctatgtc agccaaacca atcagcgaga acaccttatt
     3601 ctatggcgac aatctaaaag tcctgcgcga atatctccct gacgaaagca tcgacctgat
     3661 ctacctcgac ccgcctttca actccaaccg caactataac gtcctcttca gagacgag
//
LOCUS       DASZAK010000315         4990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_540505, whole genome shotgun sequence.
ACCESSION   DASZAK010000315 DASZAK010000000
VERSION     DASZAK010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540505"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1293)
                     /locus_tag="VGT44_05590"
     CDS             complement(34..1293)
                     /locus_tag="VGT44_05590"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEV2580306.1"
                     /translation="MHNETAVINPYHMAVQQFEDAAEHLHLSEDMREILRQPKRELVV
                     NFPVRLDNGRIKTFTGYRVQHNVNRGPAKGGIRYSPDVTLDEVKALAMWMTWKCAVVG
                     IPFGGAKGGVICDPKNMSPAELERLTRRYATEISIIIGPHGDIPAPDVNTNSQIMAWI
                     MDTYSMHEGYSIPAVVTGKPLSIGGSEGRNDATATGVLFVTRQAAKRMGMPLRGARVS
                     IQGYGNAGSVAARLFHNEGCKIVAVSDTRGGIYNEAGLDPASVLRYKQERGSVVGFPG
                     AQTVRVQDVLEVPCDVLIPAATEGVITKENADRVQARIVSEAANGPTTPQADKILFDK
                     GIVVIPDILANAGGVTVSYFEWVQDLQSFFWGVEEITDKLEVIMNRAFAAVAEKADQS
                     SCDMRLAANMLAISRVAEATQIRGIYP"
     gene            complement(1368..2864)
                     /gene="gatB"
                     /locus_tag="VGT44_05595"
     CDS             complement(1368..2864)
                     /gene="gatB"
                     /locus_tag="VGT44_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV2580307.1"
                     /translation="MTMVDTGVMIDYEVVIGLEVHAQLLTKSKMFCSCSTDYAYAAPN
                     THVCPVCMGMPGVLPVINQEAVAYTIMTALALNCSIPEYSKFDRKNYPYPDLMKGYQI
                     SQYDIPLSKDGYLTVDYEGQSRRIGITRVHLEEDTARLLHHEGYSLIDINRSGTPLME
                     IVSEPDMRSPVEARLYMQKLREILVYLGVSSGRMEEGSLRCDANISLRPRGQEQLGVK
                     TEIKNMNSFRSVERALIYEAQRQVEMLRAGQVIHQETRGWVETKGVTVGQRSKEQAHD
                     YRYFPEPDLPPLVVSRAWVEELRAGLPELPDARRVRFMQEYGLSAQDANILTEDKALG
                     DYFEQVLAVPPASPSRDVKARAKAAANLVLNDVARLLKAHDTGVRENPLTPAASANLL
                     DLLDANRITGKQAKEIIDEAFTTGTMPETIVKERGIQPPISDEGELERIIEGVIANNA
                     KAAADYRSGKVNALQSLIGQVMKQTRGQAKADSVRAILIKKLDETRSA"
     gene            complement(3084..4061)
                     /locus_tag="VGT44_05600"
     CDS             complement(3084..4061)
                     /locus_tag="VGT44_05600"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2580308.1"
                     /translation="MSTNWGVIGAGFIANRAVIPAIQAVSDARVLAVASLDEQRAQAA
                     ADRFAIPRVYHDYAELLDDLDVQIVYIALPNHLHREWTIRAAQHGKHVLCEKPLALNA
                     AECDEMIHACRQAGVLLMEAAMYRFHPRMQALKAMLDAGEAGDLRFLHSAFSFPFNAP
                     GNYRAFPIFGGGALLDIGSYCINAARWLTGSEPTSAQAAISYSRSGIDLSASAILQFG
                     DQAAGGNPFSAHIQCSFAAAEHQVIEVVGSRAAITAPLAFTAWREDATALLIQRDSLF
                     EEWNFPSADPYRLMVEHFTDCVLGRSELLFPPEDGRATMRVIDMLKRSE"
     gene            complement(4075..>4990)
                     /locus_tag="VGT44_05605"
     CDS             complement(4075..>4990)
                     /locus_tag="VGT44_05605"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2580309.1"
                     /translation="LTTGAVAVIAAIVTGRRYLRWKQQQRQRLLSASRLLETSRGVVE
                     YQVEGTGPVVLLLHGSPGGYDQGMALAHAIDLNGFTLLSPSRPGYRRTPLSSGETPEA
                     QADLYAAALDALDISQVVVVALSGGGPSALQFVQRYPQRCQALLMLCALSQNYTEEGV
                     YLSLPPVRRLFKHLLEWLIVFDPFLFLLSGLIRRISEEARSVEFIESLFMNSSQTPGY
                     RNDMRQFESLPSYAFQDIALPTLIVHGTADIDVPFRQAKELAAAIPGAQLAAIEGADH
                     LSTLARENAVVAIRSFLQHLIVRQMEKR"
BASE COUNT          954 a         1450 c         1539 g         1047 t
ORIGIN      
        1 accacggtac ccctacatcg ccttgaccgg cttttacggg tagatgccgc gaatctgggt
       61 ggcttcggcg acgcgcgaga tggcaagcat gttggcagcc aggcgcatat cgcacgagga
      121 ctggtctgct ttctcggcga cggcggcaaa ggcgcggttc ataatcacct cgagcttatc
      181 ggtgatctcc tcgacgcccc agaagaagct ttgcaggtcc tgcacccatt caaagtagct
      241 gacggtcacg ccgcccgcgt ttgccagaat atcggggatg accacgatcc ccttgtcaaa
      301 gaggattttg tcggcttgcg gcgtggtcgg gccgttggcg gcctcggaga cgatgcgcgc
      361 ctgcacgcgg tcggcattct ccttcgtaat cacgccctcg gtggcggcgg gaatcagcac
      421 gtcgcagggg acttccagca catcctggac ccgcacagtc tgcgcgccgg ggaagccgac
      481 gacactgccg cgctcctgct tgtagcgcag caccgaggcg ggatctagcc cggcttcgtt
      541 gtagatgccg ccgcgcgtat cgctgacggc cacgatcttg cagccctcgt tatggaagag
      601 gcgggcggcg acggagccgg cattgccgta gccctggatg ctgacgcgcg cgccgcgcag
      661 gggcataccc atgcgtttcg ccgcctggcg cgtgacgaag agcacgcccg tcgcggtggc
      721 gtcgttgcga ccttcgctgc cgccaattga aagtggcttg ccggtcacga cggcggggat
      781 cgaatagccc tcgtgcatgg aataggtatc cataatccag gccatgatct gcgaattggt
      841 gttcacgtcg ggcgcgggga tatcgccgtg tgggccgatg atgatggata tttcggtggc
      901 gtagcggcga gtgaggcgtt ccagttcagc cggactcata tttttggggt cgcagatcac
      961 gccgcctttc gcgccgccaa agggaatgcc gaccaccgcg cacttccagg tcatccacat
     1021 cgccagcgcc ttgacctcat ccagcgtcac atcggggctg tagcgaatgc cgcctttggc
     1081 cggcccgcga ttgacattat gctgcacgcg atagccggtg aaggttttga tgcggccatt
     1141 gtcgaggcgt accgggaagt tcacgaccag ttcgcgtttg ggctggcgca aaatctcgcg
     1201 catatcctcg gaaagatgca ggtgttcggc ggcatcctcg aattgttgta ccgccatgtg
     1261 ataagggttg ataaccgctg tttcgttgtg catgggagtc attcccacct ttcttcactt
     1321 cgttgtgttt caggttcgtt ccagttgtaa atgctataaa acaccctcta cgcggagcgt
     1381 gtttcgtcga gttttttgat gagtatggcg cgcacgctgt cggctttcgc ctggccgcgc
     1441 gtttgtttca tgacctggcc gatcagcgac tgcagggcgt taaccttgcc ggagcggtag
     1501 tccgcagccg cttttgcatt gttcgcaatc accccctcaa tgatgcgttc cagttcgccc
     1561 tcgtcgctga taggcggctg gatgcctcgt tctttcacaa tcgtctccgg cattgtgccg
     1621 gttgtgaagg cttcgtcgat aatctccttt gcctgcttgc cggtgatgcg gttggcatcc
     1681 agcaggtcga gcaggttggc ggaggcagcg ggcgtaagcg ggttctcccg cacaccggtg
     1741 tcgtgcgctt tgagcaggcg cgcgacatcg ttcaagacca ggttggccgc tgctttggcc
     1801 ctggccttca cgtccctgct tggagacgcg ggaggaaccg cgaggacttg ctcgaagtag
     1861 tcgcccagcg ccttatcctc ggtgaggatg ttggcatcct gggcggacag gccatattct
     1921 tgcataaagc ggacgcggcg agcgtcgggc aattcgggca ggccggcgcg cagttcctcg
     1981 acccaggcgc ggctgaccac cagtggcggc agatcgggtt cggggaagta gcgatagtcg
     2041 tgggcctgct ccttgctgcg ctggccgacg gtgacgccct ttgtctcgac ccagccgcgc
     2101 gtctcttgat ggatgacctg gccggcgcgc agcatctcaa cctggcgctg cgcctcgtat
     2161 atcagggcgc gctcgaccga acggaagctg ttcatattct taatctcggt cttgacacct
     2221 agctgctcct gcccgcgcgg gcgcagggag atattggcgt cgcagcgcag gctgccctcc
     2281 tccatacggc cagaggaaac ccccaggtag accagtatct cgcgcagctt ttgcatatag
     2341 aggcgcgcct caacgggact gcgcatatcc ggctcgctga cgatctccat cagcggcgta
     2401 ccagaacggt tgatgtcgat caggctgtag ccttcgtggt gcagcagtcg cgccgtatct
     2461 tcttccaggt gaacgcgcgt gatgccgata cgacgactct gcccctcgta atcgacggtc
     2521 aaatagccgt ctttgctgag tgggatatcg tactgcgaga tttgataccc cttcatcaga
     2581 tcggggtatg ggtaattctt gcggtcaaac ttggagtatt cgggaatgct gcaattgagc
     2641 gcaagcgccg tcatgatggt gtacgccacg gcctcctggt tgatgacggg cagcacgccg
     2701 ggcatcccca tgcagaccgg gcagacgtgc gtattggggg cagcgtaggc gtaatcggtg
     2761 ctgcacgaac agaacatctt gcttttcgtc aacagttgcg cgtgaacttc cagtccaatc
     2821 acaacttcat agtcgatcat cacgccggta tccaccatcg tcattgctca aaacctcttc
     2881 tattttcaga cacgctgtct atgcttctgg tttacctagc atagcgtttt cgtggtgaat
     2941 agtcaataag aacagccctt ttcgcaatta actcagtcgt ctttctatgt acctcaatac
     3001 gaggatacga gcaacgaaac acaggtgtgt cactaatggg agcgatgaga acacgaaacg
     3061 atcacaaaag gagtttcgtg aggttactct gacctcttta acatatcaat tacgcgcatt
     3121 gtcgccctgc catcctccgg aggaaacaac aactcgcttc gtccaagtac gcaatccgtg
     3181 aaatgctcca ccatcaagcg gtaagggtcg gcggacggga agttccattc ctcgaagagg
     3241 ctgtcgcgtt ggatgagcag cgcggtggcg tcttcgcgcc aggcggtgaa ggccagcggc
     3301 gcagtaatgg cggcgcggct gccgacgacc tcgattacct ggtgttcggc ggcggcaaaa
     3361 ctacactgga tatgcgcgga gaacgggttg ccgccggcgg cctggtcgcc aaattgcaaa
     3421 atggcgctcg cgctcaagtc gataccactc cggctatagg aaatagcggc ctgcgccgag
     3481 gtcggctcgc tgccagtgag ccagcgcgcg gcattaatac agtagctgcc gatgtcgagc
     3541 agcgcgcccc cgccgaatat ggggaaggcg cgatagttgc cgggcgcgtt gaatgggaac
     3601 gagaaggcgc tgtgcagaaa acgcaggtcg cctgcctcgc ccgcgtcgag catggctttg
     3661 agcgcctgca ttcgcgggtg aaagcgatac atggccgcct ccatcagcag cacaccggcc
     3721 tgtcggcagg cgtggatcat ctcgtcacac tctgcggcat tcagcgccag gggcttttcg
     3781 cagaggacat gcttgccgtg ttgcgccgcg cggatagtcc actcgcgatg caggtggttg
     3841 ggcagcgcga tgtagacgat ttgcacgtcg agatcgtcaa gcaactcggc gtagtcatga
     3901 tagacgcgcg ggatggcaaa gcggtcggcg gcagcctgcg cgcgctgctc gtccaggctg
     3961 gcgacggcca gtacacgggc gtcgctaaca gcctgaattg cagggataac ggcgcggttg
     4021 gcgatgaagc ccgcgccgat gacgccccag ttcgtggaca tgacccgccc cctgttatct
     4081 tttctccatt tggcgcacaa tgagatgttg aaggaagctg cggatggcga cgacagcatt
     4141 ttctctcgcc agtgtagaga gatggtcggc gccctcgata gccgccagtt gtgcgccagg
     4201 gatggccgcc gccagctctt ttgcctggcg gaaagggaca tcgatgtcag ccgttccatg
     4261 cacaatcaga gttggcagcg cgatgtcctg gaatgcatag gacggcaggc tttcgaattg
     4321 tcgcatgtcg tttctgtaac ctggggtttg agatgaattc atgaagagtg actcgatgaa
     4381 ctcgaccgag cgcgcctctt ccgatatgcg ccggatcaga cctgagagca ggaacaggaa
     4441 tggatcaaac acgatcagcc attcaagcag gtgtttgaac aggcgccgga cgggaggcag
     4501 cgagagatag acgccttctt cggtgtagtt ctgcgatagg gcgcagagca tcagcaacgc
     4561 ctggcagcgc tgcgggtaac gctgcacgaa ttgcagggct gacgggccgc cacccgacag
     4621 cgccacgacg accacctgcg atatatccag cgcatccagg gcagctgcgt agaggtctgc
     4681 ctgtgcctct ggcgtctctc cgctgctcaa aggtgtgcgg cgatagccag ggcgcgaagg
     4741 cgacagcagc gtaaagccat tcaggtcgat ggcatgagcc agcgccattc cctggtcata
     4801 gccgccaggg ctgccgtgca gcaacaacac gaccgggcct gtcccttcaa cctggtattc
     4861 cacaacgccc ctggacgttt ccagcagacg gctcgcagag agtaaacgct ggcgctgttg
     4921 ttgcttccac cgaagatagc gccgccccgt tactatagca gcgataactg caacggcacc
     4981 agtggtcaga
//
LOCUS       DASZAK010000316         5411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_7979, whole genome shotgun sequence.
ACCESSION   DASZAK010000316 DASZAK010000000
VERSION     DASZAK010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7979"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1423
                     /locus_tag="VGT44_05610"
     CDS             236..1423
                     /locus_tag="VGT44_05610"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEV2580310.1"
                     /translation="MVTQIKYERVAGAAQPIASLASNMPGATAVNTFTFDALPLQLGG
                     SLGPVTLAYETWGTLNATGDNAILITHALTGNTHAHDAERPNDTKIAWWNPLIGPGRF
                     FDTSRYFVICSNILGGCYGSTGPSSIDPDTGQPYRMNFPVVTIQDMVRAQRRLIAHLG
                     VRELAMVAGGSIGGQQALEWAFAYPELVRNVIVVAATAALTAQAIAFSEVQRQAILID
                     PRWQSGNYSPDQGPDAGLAIARMLAMITYQSEEGMEQRFARQPARRSPGQTPVAPGGY
                     GDLGKRFDVENYLYYQGDALVRRFDANSYLYISRAMDLYDAGEYYSSLEAALRRVSSK
                     ALFIGIRSDFLFPAARVRLLSEQARDAGADATYVELDSPHGHDAFLKEWKQMNEALNR
                     IKE"
     gene            1433..2755
                     /locus_tag="VGT44_05615"
     CDS             1433..2755
                     /locus_tag="VGT44_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV2580311.1"
                     /translation="MALNSNKQYGFETLSLHAGQETADSATNARAVPIYQTTSFVFNS
                     PEHAADLFGLRQFGNIYTRIMNPTQDAFERRIAALEGGVGALATSSGQAAETLAILNI
                     AGAGDEIVSSASLYGGTYNLFHYTLPKIGVTVRFYDSRAPESLRAVINDKTKAVFAET
                     LGNPRLDTLDIRATADIAHEYGVPLILDNTLPTPYLIQPFKHGADIVVHSATKFIGGH
                     GTSIGGVIVDSGAFDWTNGRFPGFVEPDPSYHGLRFAEALGPLAYIIKARVQLLRDIG
                     PAASPFNDWLFLQGLETLPLRMERHSQNALRIAEFLETHPAVNWVLYPGLKSHPQHDL
                     AVKYHTHGFGAIIGFGIKGGLEAGKQLIRHVELFSHLANVGDAKSLIIHPASTTHSQL
                     TVDEQYETGVTPDFVRLSVGLETVDDLLDDLDQALKAAVASVGEAASV"
     gene            2774..4384
                     /locus_tag="VGT44_05620"
     CDS             2774..4384
                     /locus_tag="VGT44_05620"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV2580312.1"
                     /translation="MEHSVSVQILKFGGTSVGSGERIRHVAKVAAGHHHQRSEEGFPV
                     VVVSAMSGITDQLLRIARAVFSGEEQTWRDELADLAHKHRQAAQEAVHDEHARQALLA
                     DLENAFAGIEQDCICAFLLNPGDRDSADAIGATAGAYAWALRAATIGAWGERLSVLVL
                     AAACRDVGLRAEAVREEVIITAEIQCETLPSTGTTVTGADPLPTQTRLNAQRLVLPLI
                     EQGIVPIAPGFIGRSARGDVVTLGRNGSDYSATVIGDALDCVEVTIYTDVDGVLSADP
                     RIVANARLLPHLSYAEAARLSWFGAKVLHPRTLIPVAHRNIPVRVRNTFRPHTRGTLV
                     GPERHARSGAAAITVRRNLALITMENTDLFGAAENAGQVFELAARAGAAPVAICSSSG
                     QHLSFIVEEQAVEPVVALLQHDMGSWRVLVRRGLAACACIGSGFTSDPTSPARAITAL
                     AHEHIPVITQGASDTGIILIVESDNSEQALRCLHRDLIAPVIPLVRHDKKDAPDPLVR
                     RGGGGRGREAGTLASPAFPAAQQKRAEP"
     gene            4381..>5411
                     /gene="asd"
                     /locus_tag="VGT44_05625"
     CDS             4381..>5411
                     /gene="asd"
                     /locus_tag="VGT44_05625"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV2580313.1"
                     /translation="MSNPSRIPVAVLGATGMVGQRFIELLQGHPWFELVALGASEQRA
                     GQPYGAVAKWRLAGSSMPEAVAALRVSASRPDAMPDDVRIVFSALPAEVASTIEADFA
                     RAGKAVFSNAKSYRMEPDVPLLIPEVNADHAQAILEQRKLRGWPGCIVTNANCSATPL
                     VMALKPLQQAFGLRKVMVTTLQAISGAGYPGLPSYDILDNAIPFISGEEEKVETETRK
                     MLGEWSVSSGFIDAQVVVSAHCNRVATREGHLERASAELARQADPQEIIAAWENYPSL
                     AQELQLPSAPAHTLVYRREHDRPQTILDRDTGRGMSVTIGRLQPCPILGYKFALLGHN
                     TIRGAAGGS"
BASE COUNT         1028 a         1851 c         1619 g          913 t
ORIGIN      
        1 gcaagggtcg cccctaccct tcataatata tgtgggtaga ctacgtaacc ctggagggta
       61 ggggcgaccc ttgcggtcgc cctggcatct tgtactcacg aaggccgccg ccgtgaggtg
      121 tcggcaaacc agggtggtac cgcgaacctt tcaacccctc gcccctgata gggagagggg
      181 ttttattgta atcattttcc ggttggaaca agcagaaagg cagaagggag tggcaatggt
      241 gacacaaatt aagtacgaga gagtggccgg agcagcgcag ccgattgctt cgcttgcgtc
      301 aaatatgcca ggagcgaccg ctgtgaacac cttcaccttc gacgcgctgc cgttgcaact
      361 gggcggttca ctcggaccgg tcacgctggc atacgagacg tggggaacgc tgaacgcgac
      421 gggcgataac gccatcctca tcacacacgc gctgaccggc aatacacatg cacatgacgc
      481 ggagcggccc aacgacacga aaatcgcctg gtggaacccg ctgatcggcc caggtcgctt
      541 tttcgacacc tcgcgctact tcgtgatctg ctcgaatatc ctgggcggct gctatggcag
      601 cacaggccca tcatctatcg accctgacac cgggcagcca taccgcatga acttcccggt
      661 cgtcaccatt caggacatgg tgcgggcgca gcgcaggctg atcgctcacc tgggtgtgcg
      721 cgaactggcg atggtagccg gaggctccat cggcgggcag caggcgctgg agtgggcgtt
      781 tgcttaccca gagctggtgc gcaacgtcat cgtcgtcgcg gctacagcgg ccctcaccgc
      841 gcaggctatc gccttcagtg aggtgcagcg ccaggccatc ctgatcgacc cgcgctggca
      901 gagcggcaac tactcgcccg atcagggacc cgacgccgga ctcgccatcg cgcgaatgct
      961 ggcgatgatt acctatcaga gcgaggaagg catggaacag cgcttcgccc gccagcctgc
     1021 gcggcgatcg cccggccaaa cgccggtcgc gccaggcggc tatggcgatc tcggcaaacg
     1081 cttcgatgta gaaaactacc tctactacca gggtgacgcg ctggtcaggc gtttcgacgc
     1141 caacagctac ctgtacatca gccgcgccat ggacctctac gacgcgggtg agtattatag
     1201 ctcgctcgaa gctgcgctac gcagggtcag cagcaaggcg ctctttatcg gtatccggtc
     1261 ggactttctt ttcccggcgg cgcgcgtacg cttgctcagc gagcaggcgc gagacgccgg
     1321 ggcagacgcg acctatgtag aattggattc tccgcacggg catgatgctt tcctgaagga
     1381 gtggaagcag atgaacgagg cgctgaatag aatcaaggag taataagaca caatggcact
     1441 caacagcaac aaacagtacg gctttgaaac gctttcactc catgccgggc aggaaacggc
     1501 ggatagcgcc accaacgcgc gcgctgtacc gatctaccag acgacctcgt tcgtcttcaa
     1561 cagcccggaa cacgccgccg atctcttcgg attgaggcag ttcggcaaca tctacacgcg
     1621 catcatgaac cccacgcagg atgccttcga gcggcgtatc gccgcgctcg aaggcggcgt
     1681 tggcgcgctg gccacctcgt ccgggcaggc agccgagacg ctggctatcc tcaacatcgc
     1741 cggggcgggc gacgagatcg tctcttcggc cagtctctac ggcggcacct acaacctctt
     1801 ccactacacg ctgccgaaga tcggcgtcac tgtgcgtttc tacgacagcc gcgcgccgga
     1861 gagcctgcgc gccgtcatca acgataaaac caaagccgtc ttcgccgaga cgctgggcaa
     1921 cccgcgcctg gacacgctcg atatccgcgc caccgccgat atcgcgcacg agtacggcgt
     1981 gccgctcatc ctcgacaaca cactgcccac accctacctg atacagccct tcaagcacgg
     2041 cgcggacatc gtggtgcatt ccgccaccaa gtttatcggc gggcatggca cctccatcgg
     2101 cggcgtgatc gtcgatagcg gcgcattcga ctggaccaat ggacgtttcc caggctttgt
     2161 cgagcctgat ccatcctatc atggcctgcg cttcgccgag gcgctcggcc ccctggccta
     2221 catcatcaag gcgcgcgtgc agttgttgcg cgacatcggc cctgctgcat cgcccttcaa
     2281 cgactggctc ttcctgcaag ggctggagac gctgcccctg cgcatggaac gccacagtca
     2341 gaatgccctg cgcatcgccg agttcctgga aacacatccc gccgtcaact gggtactcta
     2401 tcccggcctg aagagccacc cgcagcacga cctggcggtc aagtaccata cgcacggctt
     2461 cggcgcgatc atcggcttcg gcatcaaagg cggcctggaa gcgggcaagc agctcatccg
     2521 acacgtcgaa ctcttttcgc acctggccaa cgtaggcgat gccaagtcgc tcatcatcca
     2581 tcccgccagc acgacgcatt cgcagctcac agtagacgag cagtatgaaa ccggcgtcac
     2641 tcccgatttc gtgcgtctct cggtcggcct cgaaaccgtc gacgacctgc tcgacgacct
     2701 cgaccaggcc ctaaaagccg ccgtcgccag cgtcggcgaa gcagcatccg tctaagtcat
     2761 tggagcatct ctcatggaac acagcgtaag cgtacaaata ctgaaattcg gcggcacctc
     2821 ggtcgggagc ggcgaacgca tccggcacgt ggcaaaagtc gcggctggcc atcatcacca
     2881 gcgcagcgag gaggggttcc cggtcgtggt cgtttcggcc atgtccggca tcaccgacca
     2941 gctgctgcgc atcgcgcgcg cggtcttctc cggcgaggag cagacctggc gcgatgagct
     3001 ggccgacctc gcccacaagc atcgccaggc tgcccaggag gctgtccatg acgagcacgc
     3061 ccggcaggcg ctgctggccg acctcgagaa cgccttcgct ggcatcgagc aggattgcat
     3121 ttgcgcattc ctgcttaacc ctggcgatcg cgactcggct gacgcgattg gcgcgaccgc
     3181 cggggcgtat gcctgggcat tgcgcgcggc aaccatcggc gcgtggggcg aacggctctc
     3241 tgtgctggtg ctcgcggcgg cctgccggga cgtgggactg cgtgctgagg cagtgcgcga
     3301 agaggtcatc atcaccgccg aaattcagtg cgaaacgctg ccttccaccg ggacaaccgt
     3361 cacaggcgct gaccccttgc caacgcagac aaggctcaac gcccaacggc ttgtcctgcc
     3421 actgatcgag cagggcattg ttcccatagc tcctggattt atcgggcgtt ccgcgcgggg
     3481 cgatgtcgtc acgctcggtc gcaacggctc ggactattcc gctacagtca tcggcgatgc
     3541 gctggactgc gtggaagtga cgatctacac cgacgtggac ggcgtgctga gcgccgaccc
     3601 gcgcatcgtc gccaacgcgc gcctgctgcc tcacctttcg tatgccgagg ccgcgcggct
     3661 ctcctggttt ggcgcgaaag tcctgcaccc gcgcacactc atcccggtgg cgcaccgcaa
     3721 catccccgtg cgcgtgcgca ataccttccg cccgcacacg cgcggcacgc tcgtcggccc
     3781 cgaacgccac gcgcgcagcg gggcagccgc catcaccgtg cgccgcaacc ttgccctgat
     3841 tacgatggag aataccgacc tgttcggcgc ggcggagaat gccgggcagg tcttcgagct
     3901 ggccgctcgc gccggggccg cgcccgtagc catctgctcc tcgtccggcc aacacctctc
     3961 cttcatcgtc gaagaacagg cggttgagcc ggtcgtcgcg ctcttgcagc acgacatggg
     4021 cagctggcgt gtactggtac gccggggatt ggcggcctgc gcctgcatcg gctccggctt
     4081 cacgtccgac ccgacgagtc cagctcgcgc catcaccgcc ctggcgcacg aacacatccc
     4141 cgtcatcacc cagggcgcgt ccgacaccgg cattatcctg atcgtcgaat ccgataatag
     4201 cgaacaggcc ctgcgctgcc tgcaccgcga cctgatcgcc cccgtcatcc ccctcgttcg
     4261 ccacgataaa aaagacgccc cggaccccct cgtccgtagg ggcgggggtg gacgcggaag
     4321 ggaggcgggg acgcttgcgt cgcccgcgtt cccggcagcg cagcagaaac gagcagaacc
     4381 atgagcaacc catcacgcat ccctgtcgcc gtcctcggcg cgaccggcat ggtcgggcag
     4441 cgtttcatcg aactgctgca aggacatccc tggtttgaac tggtcgcgct gggcgcatcg
     4501 gagcagcgcg ccgggcagcc ctacggcgcg gtcgcgaaat ggcggctggc cggtagctcc
     4561 atgcccgaag cggtcgctgc tctgcgcgtg agcgccagtc gccccgacgc catgccggac
     4621 gacgtacgca tcgtcttctc cgccctcccg gcggaagtgg cctctactat cgaggcggat
     4681 ttcgcccgcg cgggcaaggc tgtcttcagc aacgccaaat cctaccgcat ggagccggac
     4741 gtgccgctgc tcatcccgga ggtcaacgcc gatcacgcgc aggccatcct tgaacagcgt
     4801 aagctgcgcg gctggcccgg ctgcatcgtc accaacgcca attgttccgc gacccccctg
     4861 gtgatggcgc tcaagccctt gcagcaagcc ttcggcttgc gcaaggtgat ggtcacgacg
     4921 ctgcaagcca tctctggcgc gggctacccc ggcctgccct cctacgacat cctcgataac
     4981 gccatcccct tcatcagcgg cgaagaggaa aaagtcgaga ccgagacgcg caagatgctc
     5041 ggtgaatggt cagtaagcag tggattcatc gacgcgcagg tcgtcgtcag cgcgcactgc
     5101 aaccgcgtcg ccacacgcga aggccacctc gaacgcgcca gcgccgaact cgcgcgccag
     5161 gccgacccgc aggaaatcat cgctgcctgg gagaactatc catcgctcgc ccaggaacta
     5221 caactgccca gcgcgccagc gcataccctc gtctaccgcc gcgagcatga ccgtccgcag
     5281 accatcctcg accgcgacac ggggcgcggc atgagcgtca cgataggccg cctgcagccc
     5341 tgtcccatcc tgggctacaa gtttgcgctg ctcgggcata atacgatacg cggcgcggcg
     5401 ggcgggtcga t
//
LOCUS       DASZAK010000317         2063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_327552, whole genome shotgun sequence.
ACCESSION   DASZAK010000317 DASZAK010000000
VERSION     DASZAK010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2063
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327552"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /gene="panD"
                     /locus_tag="VGT44_05630"
     CDS             <1..316
                     /gene="panD"
                     /locus_tag="VGT44_05630"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEV2580314.1"
                     /translation="AADIYPYEKVQVVNCNNGTRLETYTIAGARGSGVICLNGAAARM
                     AAEGDIVIIISYAQYTEQEIRALEPHVVFVDAENHITETKHVPLLEMLPPSESVLAEA
                     TF"
     gene            complement(420..1520)
                     /locus_tag="VGT44_05635"
     CDS             complement(420..1520)
                     /locus_tag="VGT44_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV2580315.1"
                     /translation="MQTYGPPILVGIAALAIISATALDAFEVMILPRRVARRLRLATI
                     INLLAWTPWSLIGRRIKNRNRRENFLGVYGPVGLIVLLMIWAAGLIVGYALLQWAFGS
                     AFAAPEKNPSFGTDLYVSGTTFFTLGLGDVIPVSGIARALTVIETFTGFGLLAIVIGY
                     LPVLYQAFSRREVNISLLDARAGSPPTAVELLRRYGQHQNQEALSQFLYEWERWSAEL
                     LESHLSYPSLAYYRSLHDNQSWLAALTMILDACALIMVGIDGMPEKRARLTFAMARHA
                     AVDLSQIIRAEPHECHTDRLPPGALGSVRAMLSDSGLTPHISEHADEKLLKYREMYEP
                     YVNALAEQLLMELPPWMPTEGAKDDWLTSADE"
     gene            complement(1663..>2063)
                     /locus_tag="VGT44_05640"
     CDS             complement(1663..>2063)
                     /locus_tag="VGT44_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2580316.1"
                     /translation="EMPNDLPETMNRTAIKLIMIVEDDEQIGAFIVQLIYQETRHFAI
                     HHATPHEALDWAAQHIPHLFILDYGLPEMNGLELHDQLRAFDHLQDARSILISAISPP
                     QTELKKRNMVFLAKPFNVLKLINLVEQLLN"
BASE COUNT          475 a          565 c          562 g          461 t
ORIGIN      
        1 agccgccgat atctacccct acgaaaaagt gcaggtggtc aattgtaaca atggcacgcg
       61 cctggagacc tacaccatcg cgggagcgcg cggctccggg gtcatctgcc tgaatggggc
      121 tgccgctcgt atggcggctg aaggcgatat cgtcatcatc atcagctacg cccagtacac
      181 cgagcaagaa attcgcgcat tagagccgca cgtcgttttt gtcgatgccg agaatcacat
      241 cacagaaacc aaacacgtgc cgcttctcga aatgctcccc ccatccgaat cagttttagc
      301 cgaagccacg ttctagcggc ttctcagtaa tcttcattca actgcaacgg ttgaatgaat
      361 tactgaacat cctcttccta ccaaacgttc gtcaggtatc attcctcttt ccagggcaat
      421 cattcatccg cgctagtcag ccaatcatcc tttgcgcctt cggtgggcat ccagggcggc
      481 agttccatca agagctgctc ggccagcgcg ttgacatagg gttcgtacat ctcgcgatat
      541 ttcagcagct tctcgtcggc gtgctcactg atatgcggcg tcagcccgga gtcgctcagc
      601 atggcccgca ccgaccccaa cgcgccgggc ggcagccggt ccgtatggca ttcgtgcggc
      661 tcggcgcgga tgatctgcga gaggtcaacg gcggcgtggc gcgccatcgc gaaagtcaac
      721 cgcgcgcgct tttcgggcat accatcgatg cccaccatga taagcgcgca ggcgtccagg
      781 atcatcgtca gggctgccag ccaggactgg ttatcgtgca gcgagcgata ataggccagc
      841 gatggatacg aaagatggct ttccagcagt tccgccgacc agcgttccca ttcataaagg
      901 aactggctca gcgcttcctg gttctgatgc tgcccatagc gcctcagcag ttcgaccgcg
      961 gtagggggcg aaccggcgcg ggcatcgagc agcgagatat tcacctcgcg ccgcgagaag
     1021 gcctggtata ggacgggaag atagccgatc acaatcgcca ggagaccgaa gccggtgaat
     1081 gtctcaatca ctgtcaaggc gcgcgctatg ccagagaccg gaatcacatc tcccaggccg
     1141 agtgtgaaaa aggtggttcc gctgacgtac aggtcggtgc caaagctggg attcttttcc
     1201 ggcgcagcaa acgcggagcc gaaggcccat tgcagcagcg catagccaac gatcaacccg
     1261 gcagcccaga tcatcaatag cacaatcagc ccgaccggtc catagacacc caggaagttt
     1321 tcgcgccggt tcctattctt aattcttcgt ccaatcagcg accagggcgt ccacgccagc
     1381 aggttgatga tcgtggccag gcgcagtctt cgtgctactc tacgtggtaa gatcatcact
     1441 tcgaaggcat ccaacgcagt agcgctgatg attgctaagg ctgctatgcc aaccagtatc
     1501 ggcgggccgt atgtttgcat atacgtcata tgggtccttt gagaaaacct ttccgattcc
     1561 actttatgac aggttagtaa gataatgcta ttataactca cagcgatttg ccgtggtttg
     1621 gaaaatgaaa cggtcataga agctggtgcg gtgcgatctg aatcagttga gaagctgctc
     1681 gacgagatta atgagtttga ggacattaaa tggtttagcg agaaagacca tattccgctt
     1741 cttgagttca gtctggggcg gactgattgc gctgatcaag atcgaccttg catcctggag
     1801 atggtcgaag gcacgaagct ggtcatgcaa ttccaggcca ttcatctctg gtaaaccgta
     1861 gtccaggata aatagatgcg gtatgtgctg ggccgcccag tcgagggcct cgtgaggcgt
     1921 agcatgatga attgcgaaat gccttgtttc ctggtaaata agctgcacaa tgaaggcgcc
     1981 gatctgttca tcatcttcaa caatcatgat gagtttgatt gcagttctat tcattgtttc
     2041 aggtaggtcg tttggcattt cgt
//
LOCUS       DASZAK010000318         4332 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_42977, whole genome shotgun sequence.
ACCESSION   DASZAK010000318 DASZAK010000000
VERSION     DASZAK010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42977"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..2425
                     /locus_tag="VGT44_05645"
     CDS             95..2425
                     /locus_tag="VGT44_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HEV2580317.1"
                     /translation="MTNSDSGASLAAPQVEETLPIAELARTLAVELHLSREQITRTVT
                     LLDEGNTIPFIARYRKEVTGSLDEVQIQAIADRAGALRALYERKADVRRLIDVQGKLT
                     PELANAIAASVNLQEVEDLYLPYRPKRKTRASVAREKGLAPLADLILQQPVLRGDAET
                     ILEEQARPYLDADKGVETSLEAYAGARDIAAEVMAEDATVRGSMRAAFFKRSTLGAKV
                     ADPDKIAEKDPNGVYQLYYTFNENITKLVPHRVLALNRAEREDVLRISISLPYEEAQP
                     VMTASYSIKAASPFAGQLAGAMEDGYKRLLAPAMEREARVELTRKAEEHAIHIFAANL
                     RNLLLQPPLRGRKALGIDPGIRTGCKLTIVDETGKYLESDTIYLHQSEKATQTLRRLI
                     ARHSIDVIAIGNGTASRETEQLVASLIKELEQETGKQGKIGYVIVNEAGASVYSASEA
                     ARQEFPTLDVTQRGTISIARRLQDPLAELVKIDPKAVGVGLYQHDVDQKELADMLDRV
                     VVSCVNFAGVEVNSASAALLKHVSGINTRVANAIVKYREEHGPFASRQALQKVSGLGP
                     ATFVQAAGFLKVSNGSEPLDNTFIHPESYPAAHALLAMLPADDEKGKAMKPAERVAQF
                     RQLVKLQIGRNTSSKGNRSTGGDSEHYAWDEIARKIGVGLPTLNDILDNLEKPGLDPR
                     DTLPAPILRHDVLKMEDLQPGMVLQGTVRNVVDFGAFVDIGVKQDGLVHISEMADRYV
                     RDPLSVVAVGQVVQVRVIGVDTKRGRVQLSMKEIKK"
     gene            2422..4266
                     /locus_tag="VGT44_05650"
     CDS             2422..4266
                     /locus_tag="VGT44_05650"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV2580318.1"
                     /translation="MKVTAERYSFARYLNARYVLDPSFSPDGSRLTFLTDITGVYEVW
                     SVPVDRQATTPAWPEQLTFLGERVSSATYSPARPELLVVADTGGSERTQLYLMGEDGA
                     EFTPLTSNPEAIFGFGAWSPDGTRFVYTSNERDARYFDLYEYTLKVGQARLLHQSDYY
                     NQALRYSPDGRSVLVQRVHSNVHLQLLLLDTITGDLRPLTPGVVERQGEHNYPHWSAD
                     GRGLYLASNRGRQFSTLAWLELATLEMTYLRDDPWDIEGLEISQDGKRMALLFNKEGY
                     SSVELFDVSQGWEARRELPAPDLPEGVVMGMTWAQDGQRLAIACYPADDAVDVWVWDI
                     PEQQLWRATHSSLGGIPRATLVAPTLAHYPTFDGRQIPAFLYLPQGKEARNLPIIIDV
                     HGGPEGQSRPFFNPVTQYFVGQGYAVLKPNVRGSVGYGYEYQSLDDVRLRMDSVADLQ
                     HAALWLRDSGIADPRRIAVMGGSYGGFMVLSAITTYPELWAAAIDIVGVANFVTFLEN
                     TGPWRRKHRESEYGSLEHDREFLERISPINHVDNITAPLFVIHGANDPRVPVGEAEQI
                     VAALRARGVPVEYLRFEDEGHGLIKRANKLVAYPTVARFLEEHVGNTP"
BASE COUNT          898 a         1366 c         1309 g          759 t
ORIGIN      
        1 agagtagcca gggcgatgct tgccatcgtc ctggccacta tgttactatc ttctctgacg
       61 caaccataca acccatagaa ggtaggcaac atcgttgacg aacagtgatt ccggcgcatc
      121 gctcgctgcg ccgcaggttg aagagacgct ccccattgca gaactggcgc gcaccctcgc
      181 ggttgaactc catctgagca gagagcagat tacccgcacc gttactctgc tggacgaggg
      241 taatactatt ccgttcattg cgcgttaccg caaagaagta acgggcagcc tcgacgaggt
      301 gcagattcag gccatcgccg accgcgcggg cgcgctgcgg gcattgtacg agcgcaaggc
      361 cgacgtgcgc cgcctgatcg acgtacaagg caagctgacg ccggaactcg caaacgcgat
      421 cgccgcatcc gtgaatctgc aagaggtgga agacctctac ctgccctatc gccctaaacg
      481 caaaacgcgc gccagcgtcg cgcgcgaaaa agggttggcg cccctggcag acctgatcct
      541 gcaacagcct gtgctgcgcg gggatgccga gacaatactc gaagagcagg cccgaccata
      601 cctggacgcc gacaaaggcg ttgaaacaag cctggaagcc tacgccgggg cgcgggatat
      661 cgcagccgag gtgatggccg aggatgccac cgtgcgcggg agtatgcgcg cagccttctt
      721 caaacgctcg acgcttggcg cgaaggtggc tgaccccgat aaaatcgccg aaaaagaccc
      781 caacggcgtc taccagctct actatacatt caacgaaaac atcacgaaac tcgtgccgca
      841 tcgcgtgctg gcgctcaacc gggcggagcg cgaagacgtg ctgcgtatca gcattagcct
      901 gccctatgaa gaggcgcagc cggtcatgac cgcaagctat tccatcaagg cggcctcgcc
      961 ctttgcgggt caactcgcag gcgcgatgga ggacggctac aagcgcctgc tcgcaccggc
     1021 catggagcgc gaggcgcgcg tggaactgac ccgcaaagcc gaggaacacg ccattcatat
     1081 tttcgccgcc aacctgcgca acttgctgct ccagccgccc ctgcgcgggc gtaaggcgct
     1141 gggcatcgac cccggcatcc gcacaggctg caagctgacc atcgtggacg agacgggcaa
     1201 gtatctcgaa tcggatacaa tctacctgca ccagtccgaa aaggcgacac agacgctgcg
     1261 caggctgata gcgcggcact ccatcgatgt catcgccatc ggcaacggca ccgcctcgcg
     1321 cgagaccgaa cagcttgtcg cctcgctcat caaggaactg gaacaagaaa ctggcaagca
     1381 gggcaagatc ggctatgtga tcgtcaacga ggctggcgcg tcggtctatt cggcctcgga
     1441 agcggctcgc caggaatttc cgacgctgga tgtcacgcag cgcggtacca tctccatcgc
     1501 gcgccgcctg caagaccccc ttgcggaact ggtcaagatc gacccgaagg cggtgggcgt
     1561 gggcctgtac cagcacgacg tagaccagaa ggaactggct gacatgctcg accgcgtcgt
     1621 ggtctcctgc gtcaacttcg ccggggtcga ggtcaattcg gcttcggctg ccctgctcaa
     1681 gcacgtctcc ggcatcaata cgcgcgtcgc caacgccatc gtcaagtatc gcgaggaaca
     1741 cggcccgttc gcgtcgcgcc aggcattgca aaaggtgtca gggcttggcc cggccacctt
     1801 cgtgcaggcc gccggtttcc tcaaggtctc caacggcagc gagccgctcg acaacacctt
     1861 cattcacccc gaaagctacc ctgctgcgca cgctttgctg gctatgttgc cagcagatga
     1921 cgagaaaggc aaggcaatga agccagcaga gcgtgtcgcg cagttccgcc aactggtcaa
     1981 gctgcaaatc ggtcgtaaca cgagtagcaa aggcaaccgt tcgacgggtg gagatagcga
     2041 acattacgcc tgggacgaga ttgcgcgcaa gatcggcgtc ggtctgccca cactcaacga
     2101 catcctcgac aacctggaaa aacccggcct tgacccgcgc gacactctgc ccgcgcccat
     2161 cctgcgccac gacgtgctga aaatggagga tttgcagccc ggcatggtgc tgcagggaac
     2221 cgtgcgcaac gtcgtggatt tcggcgcgtt cgtcgatatc ggcgtcaagc aggatggcct
     2281 ggtgcatatc tcggagatgg ccgaccgcta cgtgcgcgac ccactctccg tcgtggccgt
     2341 cggccaggtg gtacaggtgc gcgtgatcgg cgtggacacg aagcgcggca gggtacaact
     2401 gagtatgaag gagataaaaa agtgaaagtc acagcagaac gctatagttt cgcccgttac
     2461 ctcaatgccc gttacgtctt agaccctagc ttttcaccgg atgggtcgcg tctcaccttc
     2521 ctgaccgata taaccggcgt ctacgaggtc tggagcgtgc ctgtcgatcg tcaggcaact
     2581 acgccagcgt ggccagaaca gttgacgttt ctgggggagc gcgtcagcag cgcgacctat
     2641 tctccggcaa ggccagagct gctggtggtt gccgatacgg ggggcagcga gcgcacgcag
     2701 ctctacctga tgggcgagga tggcgcggaa ttcacgccgc tgaccagcaa tccagaggct
     2761 attttcgggt ttggcgcgtg gtcgcccgat gggacgcgct tcgtctatac cagcaacgaa
     2821 cgtgacgcgc gctacttcga cctctacgag tatacactga aagtcggaca ggcgcgcctg
     2881 ctgcaccagt ccgactacta caaccaggca ctgcgctatt cgccggatgg gcgctccgtg
     2941 ctggtccagc gcgttcattc gaatgtccac ctgcaattgc tgctgctcga tactatcacg
     3001 ggcgatttgc gacccctgac gcccggcgtg gttgagcggc aaggcgaaca caactatccc
     3061 cactggtcag ccgacgggcg cggactctac ctggcgagca atcgcgggcg gcagttctct
     3121 actctggcct ggcttgaact ggcaacgctc gaaatgacct acctgcgcga tgatccctgg
     3181 gacatcgagg ggcttgagat atcgcaggat ggcaaacgca tggcgctctt gttcaacaaa
     3241 gaaggatatt ccagcgtaga actgttcgat gtctcgcaag gatgggaggc gcggcgagaa
     3301 ctgcccgcgc ccgacctgcc ggaaggcgtg gtaatgggca tgacctgggc gcaggacggc
     3361 cagcggctcg ccattgcctg ctacccggcg gatgatgcgg tggacgtatg ggtgtgggat
     3421 atcccagagc agcagctctg gcgcgcaacg catagctcgt tgggcggcat cccgcgcgca
     3481 acgctcgtcg cgcccacgct ggcgcattat ccaaccttcg atggccgcca gatacccgct
     3541 ttcctctacc tgccgcaggg caaagaggcg cgcaacctgc cgatcatcat cgacgtgcat
     3601 ggcggcccgg agggccaatc gcgcccattc ttcaacccgg tgacgcagta cttcgtcggg
     3661 cagggctatg ccgtcctcaa acccaacgtg cgcggcagcg ttggatatgg ctacgagtat
     3721 cagagcctgg atgatgtgcg tctgcgcatg gactcggtcg cggatttgca gcacgccgcg
     3781 ctgtggctgc gcgacagcgg catcgccgac ccgcggcgca tcgccgtaat gggtggcagc
     3841 tatggcggct ttatggtgct gtccgccatc acgacttacc cggaactctg ggcagccgcc
     3901 atcgatatcg tcggcgtcgc caatttcgtg actttccttg aaaacaccgg cccctggcgg
     3961 cgcaaacatc gcgagtccga atatggcagc cttgagcatg accgcgagtt ccttgaacgc
     4021 atctcgccca tcaaccacgt ggacaacatc accgcgccgc tcttcgtcat ccacggcgcg
     4081 aacgatccgc gcgtccccgt cggcgaggcc gagcagatcg tggcagcgct gcgagcgcgc
     4141 ggcgtaccgg tcgaatacct gcgcttcgag gacgaagggc atggcctgat taaacgcgcg
     4201 aacaagttgg tggcttatcc taccgtcgca cggttcctgg aagaacacgt cgggaatacc
     4261 ccgtaggcgc cggttcacgg tgcgcaccgc cgatttatcg gcctaatggt tcgtcagcgc
     4321 cctccacagg cc
//
LOCUS       DASZAK010000319         4691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_8014, whole genome shotgun sequence.
ACCESSION   DASZAK010000319 DASZAK010000000
VERSION     DASZAK010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8014"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VGT44_05655"
     CDS             <1..877
                     /locus_tag="VGT44_05655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580319.1"
                     /translation="RLPGVGDRIAVEHERHYLVMQLRPAREERVARQGRQGETAAVAV
                     SLAELLLKHQQWPEWLDVEAAYEWGVQLCRIVARLHRMGVTLGDLDPATILVNSGGPS
                     SWAPVLLVSWPPAQQFWDGSREEYARIFPIAESLSENAFAAPETLAGQADERSDVYSL
                     GALLYLFFTRYAPASAALRQPVERSASQQSGHLPRLHNGLYNGSAFEAHSGMALVPPH
                     LFNSLISPRLEALLLRALALAQAERFPTVFALVEALESLDPAIEFVDHYEETHPARHE
                     SRIGRALEWVRGDHA"
     gene            complement(985..2091)
                     /locus_tag="VGT44_05660"
     CDS             complement(985..2091)
                     /locus_tag="VGT44_05660"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEV2580320.1"
                     /translation="MRKKIAALVIALGIFAVQALPALACGGLIAPDGDVRLQNATTLI
                     AWHNGIEHYLTTFTYAGSSTDLGWIVPLPAVPLQIQDGGAWTLQRLEIASHPVTLAFG
                     AAQTDSAAAPSATVLQRVKIEALNITVIRGSGPEIINWATGNNFFLNDDTRNHLLTYA
                     KGSPIFMAVKFDTSAAKARGQLQGDGTPILITMKIPHIWVPLEVLALDGQQVQADLFL
                     LTDNPVYTSDFSAFAGQSSVGSTIGNAQGFTMPFQEQIDASLYHDLSTDRNMGWVWPN
                     SWLTYLTLNAPSEAVTYDMGVASSGVIRLAPFGTPPMAVVDSQASHSLPSWVPEMPLG
                     TPQFLIGTIIVLTILASSVMFLRHRRRTRTELAA"
     gene            2283..2711
                     /locus_tag="VGT44_05665"
     CDS             2283..2711
                     /locus_tag="VGT44_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEV2580321.1"
                     /translation="MPKQQHILRLTLPIPPSINEQYATVNGLRVSTVVARRFKEQVKL
                     KLNEVARTGQLNESLRAALRQGYLGLFLAFYFETPLKRDLDGGLKITQDAICEHLGLN
                     DNRIVTIHLVKYIDPLHPHLDAELEAIEDWQFDEEYKLLC"
     gene            complement(2779..3759)
                     /locus_tag="VGT44_05670"
     CDS             complement(2779..3759)
                     /locus_tag="VGT44_05670"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV2580322.1"
                     /translation="MESSNTQIATLKLLDHQGIDWQRVQRSAYLIHQHFSYSYPGPVS
                     DLHQRLLILPPDHYGDQRYIVHRLDVSAEVAETTHASDEFGNHVIELLIPRVEQSIDF
                     EVWIVVERSAEHGQLLLPPTALTDPRYLEPSPLTQPDDALRTMAAALAAEIDPRDRPQ
                     ELARRINEKAHETLKYAHDVTSIHTTASEALALGQGVCQDYAHIMLALCRLCGLPARY
                     VSGHLLGEGSTHAWVEALLPAPEQDIQPGQTGAAMTLALDPTHGCETSLSYLTVAVGR
                     DYYDVAPTSGTFRAPYRGELTASKRVGPISLEYADSEGRPQGYAPTMTTQ"
     gene            complement(3821..4651)
                     /locus_tag="VGT44_05675"
     CDS             complement(3821..4651)
                     /locus_tag="VGT44_05675"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV2580323.1"
                     /translation="MQVRVGCEFRYEATWETPAVMQVQPRRDAEPRILSEEWQLTPSL
                     ASDSFTDLYHNACRRMVLPAGEQIIRYDALVEVSDEKDDVDLSAQEMRIEAMPGEVLH
                     FTLPSRYCLSDVLSDMAWQLFGSITPGYGRVQAICDWVHNNIRFQYGTSNPLTTAADV
                     CAQGVGVCRDFAHLAVTFCRALNIPARYVFGYLPDIGVPPPDAPMDFCAWMEVYLGGR
                     WFTFDPRNNEPRIGRVLVGRGRDALDVAMVTTFGSPRLAQMTVWADEVSPDGQMQEAS
                     "
BASE COUNT          869 a         1313 c         1538 g          971 t
ORIGIN      
        1 gcgactcccc ggcgttggtg atcgcatcgc ggtggaacat gaacgtcatt acctggtgat
       61 gcagcttcgc ccggcgcgcg aggagcgtgt ggcaaggcag ggaaggcagg gtgagacagc
      121 ggcggtggct gtatcgctgg cagaattgct gctcaagcat cagcagtggc cggagtggct
      181 ggatgtggag gctgcttacg aatggggtgt gcagctctgc cgcattgtcg cgcggctgca
      241 tcgcatggga gtcacgctgg gcgatctcga tcccgcgacg atcctggtaa acagcggcgg
      301 cccctcaagc tgggcgccgg tgctgctggt ctcctggcca ccggcccagc aattctggga
      361 tggcagcagg gaggaatatg cgaggatatt tcctatagcc gaaagcctat cggagaatgc
      421 ctttgccgcg ccggagacac tggcagggca ggcggacgag cgttccgatg tctattcgct
      481 tggcgcactg ctctacctgt tcttcactcg ttatgcgcct gcctccgccg cgctgcgcca
      541 gccggtcgaa cgatccgcgt cgcaacagag cggccatctg ccccggctgc acaatggact
      601 atacaacggt tccgcttttg aggcgcacag cggcatggca ctcgtgccgc cgcatctctt
      661 caacagcttg atctcgccgc gtctggaggc tttgctgttg cgcgccctgg ccctcgcaca
      721 ggcagaacgc ttccctaccg tttttgcgct ggtggaggca ttggagtcgc tcgatcccgc
      781 catagagttt gttgatcact acgaagaaac acatcctgcc cggcatgagt ccaggattgg
      841 gagggcgctg gaatgggtga ggggcgatca cgcatgatgg tgtctatgtg cgtgtgtatc
      901 ggtgaatcca tcgcccacat acccggtggc tttgcgattc aattgtgcta ccgggtacgc
      961 gggcgacgca agcgtcccta cggattatgc tgctagctcg gttcgtgtgc gtcggcggtg
     1021 gcgcaggaac attacgctgc tggccaggat ggtcaggacg atgattgtgc cgatcaggaa
     1081 ctggggggtt cccaggggca tttctggtac ccaggagggg agactatgcg atgcctggct
     1141 atccacgacc gccattgggg gcgtaccaaa gggggccagg cggatcacgc cggatgaagc
     1201 gacgcccatg tcgtaggtga cggcctcgct gggcgcgttg agggtcaggt aggtcaacca
     1261 gctattgggc cagacccagc ccatgttgcg gtcggttgac aggtcgtgat agagcgatgc
     1321 gtcgatttgc tcctggaaag gcatggtgaa cccctgagcg ttgccgatgg tgcttccgac
     1381 cgaggactgc ccggcgaagg cgctgaagtc gctagtatag acggggttgt cggtcagcag
     1441 gaagaggtcg gcctgcacct gctgtccgtc cagcgccagc acttcgagcg gcacccagat
     1501 gtgcggtatc ttcatcgtaa tcaggatggg cgtgccgtcg ccctgcaact ggccgcgcgc
     1561 ctttgccgcg ctggtgtcga acttgacagc catgaagatg gggctgccct tggcataggt
     1621 cagcaggtgg ttgcgggtat catcgttcaa gaagaagtta ttgccggtgg cccagttgat
     1681 gatctcgggg ccgctgccgc gaataacagt gatgttgagc gcctcgatct ttacgcgctg
     1741 caagaccgtc gccgatggcg cggctgccga gtcggtctgg gccgctccga atgctaaagt
     1801 aacagggtgg gatgcaatct caaggcgttg cagcgtccac gcgccgccat cctggatttg
     1861 caggggaacg gcaggcaaag ggacgatcca gcccaggtca gtgctacttc cggcgtaggt
     1921 gaacgtggtc aggtagtgtt cgatgccgtt atgccaggcg atcagggtgg tggcattttg
     1981 caggcgcaca tcgccatcgg gggcgatcaa cccgccgcat gccagggcgg gcagcgcctg
     2041 tacggcgaag atgcccaggg caatgaccag ggcagcgatc tttttgcgca tagtaatttc
     2101 tcctcacatc aggttgtgtg gtcttcatga atggttgttt tgactgcttc tcttgcgtcc
     2161 caggacttct tccactatga cggcggagat cagaaaaaag ttccctgctt gctacagtgg
     2221 gtgatacaat acctatacgc ggttgtgctg atgactcgat cataggaggg aattacgcga
     2281 agatgcccaa acagcagcac attctacgtc tgacgctccc gattccgccg agtatcaacg
     2341 aacaatatgc taccgtcaac gggctgcgcg tcagtacggt agttgccagg cgtttcaagg
     2401 agcaggtcaa actaaagctg aatgaagttg cgcgtactgg acaactcaac gagtcgctgc
     2461 gcgctgcttt gcgccagggc tacctgggtc tcttcctggc cttctatttt gagacgccgt
     2521 tgaagcgcga cctggacggc ggcctcaaaa tcacgcagga tgccatctgc gagcatcttg
     2581 gcctcaacga taaccgcatc gtcacaatcc acctcgtgaa gtacattgac ccgctgcatc
     2641 ctcaccttga tgcagaactg gaggccatag aggattggca gtttgacgag gaatacaagt
     2701 tgttgtgctg acagagggca agaagggcga ccgcaagggt acgccctact atgactacgg
     2761 ggcgcggaat cgacattgtt attgcgtggt catagtaggg gcgtaccctt gcggtcgccc
     2821 ttcactatct gcatattcca gtgaaatcgg tccgacgcgc ttgctggcgg tcagttcgcc
     2881 gcgatagggg gcgcggaagg tgccggaggt gggggcgaca tcgtagtagt cgcgcccgac
     2941 ggcgacagtg aggtagctga ggctggtttc gcagccgtgc gtcgggtcga gcgccagggt
     3001 catcgctgcg cctgtctgcc ccggctgtat atcctgttca ggcgcgggga gcagcgcttc
     3061 tacccaggcg tgggtgctgc cttcgcccaa caggtggccg gagacgtagc gtgctggcag
     3121 cccgcacagg cggcagagcg caagcatgat atgcgcgtag tcctggcaga cgccctgtcc
     3181 cagcgcgagc gcctcggatg cagtggtatg gatgcttgtt acgtcgtggg catacttgag
     3241 cgtctcatgc gctttctcgt tgatgcgccg ggccagctct tgcggcctgt cgcgcggatc
     3301 gatttcggcg gcgagcgcgg cggccatggt gcggagagcg tcgtcgggct gtgtcagcgg
     3361 cgaaggttcc aggtagcgcg gatcggtcag cgcggtgggc ggcagcagaa gctggccatg
     3421 ctcggcggaa cgctcgacaa cgatccagac ctcgaaatcg atggattgtt cgacgcgagg
     3481 gatcagcagt tcgatgacgt gattgccgaa ctcgtcgctg gcgtgggtcg tctcggcgac
     3541 ttcggctgag acatccagac gatgcacgat gtagcgctga tcaccgtagt gatcgggcgg
     3601 caaaatcaag agacgctggt ggaggtcgct gacagggccg gggtagctat agctgaaatg
     3661 ctggtggatc aggtaagccg agcgctgtac gcgctgccag tcaatcccct ggtggtccag
     3721 gagtttaagg gttgcgatct gcgtgttact gctttccaat ggttcgtgct tcatccttta
     3781 ctgcggtcgc ttcttcttcc ttcgcacttg ccttcctggt ctaggatgct tcttgcatct
     3841 gtccgtcggg cgatacctcg tcggcccaga cggtcatttg cgccaggcga ggcgagccga
     3901 aggttgtgac catcgccacg tccagtgcgt cgcgccctcg accaaccaga acgcgcccga
     3961 tgcgcggttc gttgttgcgc gggtcgaagg tgaaccagcg cccgccgagg tagacctcca
     4021 tccaggcgca gaagtccatc ggggcatcag gcggcgggac gccgatatcc ggcaggtagc
     4081 cgaagacgta gcgcgcggga atgttgaggg cacggcagaa ggtcacagcc aggtgcgcga
     4141 agtcgcggca cacgccgacg ccctgcgcgc atacatcggc ggcggtcgtg agcgggttgc
     4201 tggtgccgta ctggaagcga atattgttgt gtacccagtc gcagatggcc tgcacgcgcc
     4261 cgtagccagg cgtgatggaa ccgaacaact gccaggccat atccgagagg acatccgaga
     4321 ggcaatagcg gctgggcagc gtgaagtgca aaacctcgcc gggcatcgcc tctatacgca
     4381 tctcttgcgc gctcagatcg acatcgtctt tctcgtcgga gacctcgacg agagcatcgt
     4441 agcgaatgat ctgttcccct gccggtaaga ccatgcgccg acaggcgttg tgatagaggt
     4501 cggtaaaact atcgcttgca agcgacggcg taagctgcca ctcctcgctg aggatgcgcg
     4561 gttccgcgtc ccggcgcggc tgcacctgca tgaccgcagg tgtctcccac gtggcctcgt
     4621 agcgaaattc gcatccaact ctgacttgca tcggctcctc atctggcgac tggctgatat
     4681 tttacgctct t
//
LOCUS       DASZAK010000320         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_363375, whole genome shotgun sequence.
ACCESSION   DASZAK010000320 DASZAK010000000
VERSION     DASZAK010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363375"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..969)
                     /locus_tag="VGT44_05680"
     CDS             complement(<1..969)
                     /locus_tag="VGT44_05680"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2580324.1"
                     /translation="MLFRNIYLKTLRDYRIAILGWGLGIGLLVYVVLSSFPSLVETPE
                     ARASLVSLGPSFAWIAEPIKIGTPGGYATFKYGFTVLVMALWPLLASSRMLRGEEERG
                     SMDVLLSMPRGRGRVALEKLAAIWTALLGMGLLIGLLTYAGGMRVNADFSFGDALLSG
                     LNLALICGVFGSLALLISQFTQERGTAAGLTGGLLLIFIVLDMVHRVIPNTDWISHFS
                     PIYYYNLNKPLVPGYGINPGALLVLFALSILLSGAAVWLFARRDVGGVVAGPRFLRLP
                     ERAMRPEQALPVNAWSLRSVYTRSLGMMVVPAFWWTLAIAGFAAWMV"
     gene            complement(959..1885)
                     /locus_tag="VGT44_05685"
     CDS             complement(959..1885)
                     /locus_tag="VGT44_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580325.1"
                     /translation="MTAIIEVEALTKSYGSKRGITGVSFQVEEGEVFGFLGPNGAGKT
                     TTIRLLMALLRADAGTARIAGLDCWQQSVEIKRLIGYLPGEPSLDPSLTGGQILEYFG
                     NLRGGVDQAYLKQLIKRLDLDPSRKFRQYSSGNKRKVVLIQAFMHRPRLLILDEPTNG
                     LDPLNQQEFDRMVKEVRDEGRTVFLSSHILSEVEQTCTRVGIIREGQLVRVGGMAELK
                     DIKRYEITITFADTVSAQAFKTLNGVEQVEILPGGYALRLAMQGGADAVIKAAAQYPV
                     VSLTSHEPSLEDVFLRYYEGDGLATKEESNAV"
     gene            complement(1914..>2591)
                     /locus_tag="VGT44_05690"
     CDS             complement(1914..>2591)
                     /locus_tag="VGT44_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2580326.1"
                     /translation="GEASAQPEERERRKERILDAATTLLVRWGYRKTTIDDVAREAGV
                     GKGTIYLHWKDKNDLFRAAIWREQQRYSEDVQRRIAADPEGGLLHRIIIHAMLATLSN
                     PLMAAIIRGKSDIFNGFIGAYDPGFLNQLVGDSDAYFDQLQHAGLIRADIPASIITYL
                     LTALKVGVINSPDIFSKEQLPSMEQLTEVLSDLIRRWLEPEQPPRKSDVGKQLWTEYL
                     DKVKLSE"
BASE COUNT          612 a          749 c          685 g          545 t
ORIGIN      
        1 gaccatccac gcggcaaatc cggcaatggc tagtgtccac cagaatgcag gaacaaccat
       61 cattccgagg ctgcgtgtat acacagagcg cagcgaccaa gcattcacag gcagtgcttg
      121 ctcgggccgc atagcacgct cgggcaggcg caaaaagcgc ggccctgcca ccaccccacc
      181 gacatcgcgc cgcgcaaaga gccagacggc tgctccgctc aagaggatgc tcagggcgaa
      241 taagaccaat aaggcaccag gattaatacc gtaccccggc actaatggtt tgttcaagtt
      301 gtagtagtag atcggggaaa aatgtgagat ccaatcggta ttgggaatga cccggtgaac
      361 catatccagc acgatgaaga tcagaagcaa ccctcccgtc aatccagcag ctgtgccacg
      421 ctcctgtgta aattgggaaa tcaacagcgc aagtgaccca aagacgccac aaatgagtgc
      481 gagattcaaa ccactgagca gtgcgtcgcc aaaactaaaa tccgcattca cgcgcatacc
      541 accagcatag gtcaatagcc caatcaatag gcccatccct agtagcgctg tccagatcgc
      601 ggccagtttc tcgagtgcta ctcgaccgcg cccacgtggc atcgagagta acacgtccat
      661 tgatcctcgc tcctcctcac cacgcaacat gcgactactt gccagcagcg gccagagagc
      721 catcaccaaa accgtgaagc catatttaaa ggttgcatag cctcccgggg taccgatctt
      781 tattggctca gcgatccagg cgaaagatgg gccaagactc acgagggaag ctcgcgcctc
      841 aggcgtttca accagcgagg gaaaagaaga caaaaccaca tatacaagga ggcctatgcc
      901 taaaccccag cccagtatgg caatgcggta atcacgcagg gttttgaggt agatgttcct
      961 aaacagcatt gctctcctcc ttagtagcca gaccatcgcc ctcgtaatag cggaggaaga
     1021 cgtcctccag gctcggctcg tgactggtta agctgacgac aggatattga gctgctgcct
     1081 tgatgacagc atctgctcca ccctgcatag ccagacgcaa cgcataccca ccaggcaata
     1141 tctctacctg ttcaactcca ttcagtgtct tgaacgcttg tgcagaaaca gtatcggcga
     1201 acgtgatcgt gatttcgtag cgcttgatgt ccttcagttc ggccatacca ccgacgcgca
     1261 ccagttggcc ttcacgaatg atgcccacgc gggtgcaggt ctgttcgacc tcgctcaaga
     1321 tgtgcgagga cagaaagacg gtacgcccct catcgcgcac ttctttgacc atgcggtcga
     1381 actcttgctg gttgagtggg tccaggccat tggtcggttc atcgagaatc aacaaacgtg
     1441 ggcggtgcat gaaagcttga atcagcacta ccttgcgctt attgccactc gaatactggc
     1501 gaaacttgcg gctggggtcc aggtccagtc gcttgatcag ttgcttgaga taggcctggt
     1561 cgactcctcc gcgcaggttc ccaaagtatt ccagaatctg cccgccggtc aagctggggt
     1621 cgagcgatgg ctcgccgggg agataaccga tcagccgctt gatctctacc gactgctgcc
     1681 agcagtccag ccctgcgatg cgcgcggtgc ctgcgtcggc gcgtagcagc gccatgagca
     1741 ggcgaatcgt ggtggttttg cccgcgccgt tggggccaag gaagccaaaa acctcgccct
     1801 cttccacttg gaaggagacg ccggtaattc cacgtttgct accgtagctc ttcgtcagag
     1861 cctctacttc gatgattgca gtcatggtga ccttcctcct cttattcagg ccattattcg
     1921 gatagcttaa ctttatccag atattcggtc cagagttgtt tgcctacatc gctcttgcgc
     1981 ggtggttgct ccggttccag ccagcgacga atcaggtcgc tgagtacctc tgtcagttgc
     2041 tccatggatg gcagctgttc cttgctgaat atatcaggcg agttgatcac gccaactttc
     2101 agagcggtca gcagataggt gatgatggaa gctggaatat ctgcgcggat cagcccggca
     2161 tgttgaagct ggtcaaaata ggcatcggaa tcacctacga gctgattgag aaagcctgga
     2221 tcataggctc cgataaaccc gttgaagata tctgacttgc ccctgatgat ggcagccatg
     2281 agaggattgg aaagggtggc aagcatggca tggatgatta tgcgatgcag cagcccaccc
     2341 tcaggatcag cagcgatacg gcgctggaca tcttcgctat agcgttgctg ttcgcgccag
     2401 atggctgcgc gaaacaggtc gtttttgtcc ttccaatgta aatagatggt acccttgccc
     2461 acgccagcct cgcgagccac gtcatcaatc gtggtcttgc gatagcccca gcgcaccagc
     2521 agcgtggtgg cagcgtccag aatgcgctct ttgcgccgct cacgttcttc aggctgagcg
     2581 ctcgcctcac c
//
LOCUS       DASZAK010000321         7032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_257156, whole genome shotgun sequence.
ACCESSION   DASZAK010000321 DASZAK010000000
VERSION     DASZAK010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257156"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(294..728)
                     /locus_tag="VGT44_05695"
     CDS             complement(294..728)
                     /locus_tag="VGT44_05695"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2580327.1"
                     /translation="MSTEENKALVRRWYEEVFNQKKLAAVGEFCDPQIIEHDLPPGLP
                     AGSEGTKQLIGMFLAAFPDFHITAEDLIAEGDKVASRFTVRGTHKGGFMGIPPTGKQV
                     TMTGIQIPRIANGKMVENWLNLDMLGLLQQLGVVPPPGQASS"
     gene            complement(872..2452)
                     /locus_tag="VGT44_05700"
     CDS             complement(872..2452)
                     /locus_tag="VGT44_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEV2580328.1"
                     /translation="MQQNQPATSGIRLAELMAALSLATDLGMGQPLEYALSVCVLSVR
                     LGEALALSESELREVYYLALLRHIGCNAETYRMADLFGDELALRGDFAAVDSGHTSQV
                     VGLVIRSIQQANEGRSALHLARMIAQGLLTTPHLMKEGFTGFCEVAQRLAERLGFGEG
                     IKLALGQVFERWDGRGIPGKVKGENIALSMRIVALAQDAITFHRLEGAEAAVTMARQR
                     KGTSYDPRMVECFCQQASQLLAGLEQEPSWEVVLSLEPGVRTRLSDEQFDHACRAIAD
                     FADIKSPYTLGHSVGVAELVAQAARRCGLPEADMVALRRAGLLHDVGRVAVSAGIWGK
                     PGPLSEREWERVRMHPYYTERVLARPEMLARLGALAALHHERLDGSGYYRGLPASMLS
                     PAARILAAADVYHAMTEPRPHRPALTPESAAEQLRREARAGRLDSEATTSVLAAAGHS
                     VRSTRRELVAGLSERELEVLRLIARGHSKKLVAGQLIISEKTVDNHIQHIYNKIGVST
                     RAGASLFATEHDLLAPVE"
     gene            complement(2519..3661)
                     /gene="cax"
                     /locus_tag="VGT44_05705"
     CDS             complement(2519..3661)
                     /gene="cax"
                     /locus_tag="VGT44_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEV2580329.1"
                     /translation="MPRWLYYLLVMSVVAPILEIIWGTQLELVIFITSAIALIPLAGL
                     IGRATEDLEYYVGPIAGGLLNATFGNAPEIIIGIFALRAGLISVVKASIAGSVISNAL
                     LVLGSSLALGGWRWGRQYFDARDAGEFTAMMVMAVAGLLIPTVGSLAIGNAARIQAVS
                     VVISALLLIVYVLYLALHIFHVRAARRNPKHGGKTAPLPPPKEEEEGEEEAASGGVNP
                     RGQAIRQSPPKPWVIGLMLLVATVGTAWNSELLVGAITPVTRLLGWSPVFIGLVIIPI
                     VGNAAEHSSAIYIALRDRVDLSMAIAAGSSIQVATFVAPLLVLVSLFFATPLTLVFQP
                     LELVVLALATVLFAFISLDGESTWLAGVQLVAIYVMACVVFFFVPG"
     gene            complement(3666..4124)
                     /gene="rplI"
                     /locus_tag="VGT44_05710"
     CDS             complement(3666..4124)
                     /gene="rplI"
                     /locus_tag="VGT44_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV2580330.1"
                     /translation="MKVILLQDVDNLGKTGDLKEVANGFARNYLMPKRLAAPATPSLL
                     ANHQQRVATEKRKQEKQAEADRQQAERLSSVSLTFKARVGKQGRLYGSITSQDIAAGL
                     RDGENIVVDRRSIYLPDPIRSMGTYQVPVKIATKLEPKITVNVIDEKSVE"
     gene            complement(4314..5771)
                     /locus_tag="VGT44_05715"
     CDS             complement(4314..5771)
                     /locus_tag="VGT44_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2580331.1"
                     /translation="MNRQSESPGRTRVKRPRNKAERAHEAVVQAGMWREWVDEAGYPL
                     LPDEQPISTVRDSLYDLIPLGLREEKLIGTAPFLRLQKVKQLGFVYRVWPGATHTRYE
                     HSLGSYYLAVRALRALLQRGSNGGLFGVSISSVQTLVVAALLHDIGHYPFSHTIEELG
                     YPIVLHEKVGRSIIEKSEIATILERDYGLSPGRVADFIDPPRSQPLPADDVLLGELLS
                     GALDVDKLDYLPRDARACNVPYGGVDVARLQGALRIHPDANGQRRVVVTHKGISPLHS
                     LLHARQEMFDNIYWHHTSRAMQVMLVRAVQEALLCSALDAERLIDMDDASLLALLARA
                     EMPASARALAEELEMRRPYKGVLEVSRLAGRLYARLDALFWNAPRRRQVEQQLAAELA
                     TTLATDIADYEVLFDIPRPEKWEMDVWVTFAHPPVGMEPLVTWVEATGLQPDDLARYE
                     QHQRRIRLVAPERLRETLSSRRDDVVIPLLDRLIS"
     gene            6170..>7032
                     /locus_tag="VGT44_05720"
     CDS             6170..>7032
                     /locus_tag="VGT44_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580332.1"
                     /translation="MKLNNFWGTTRSPGLQVGMIVRLGLALTAIAGSIALAWVLLGGH
                     YDAHAGAINTLGTADQPMGLVIDKNGNAWVAEPNCNPAPVCSAPAAGAIEEFKLNGTS
                     GISHVATYLAPQLYNPTFLTVDASGNIWFTDPTNNAIGKLVPGAHPTWAKYTTGLSAS
                     AEPFGIITDKNGKIWFAERGISKIGFIDPTAGTPTIHENGGFTTNSQPFGLTMDNTGN
                     IWFTEDTVANLGTFTPTTNGTVTIHEHPTGTGANLLPAHMISADKTGNLWYSEGGTDL
                     VGEYTISGGAT"
BASE COUNT         1497 a         2148 c         1958 g         1429 t
ORIGIN      
        1 actcaaccag ccggttgata tcgccatcga tgcccgacag cggcagcacc agtccatact
       61 tcatcttgtg ttcctcctct cccttttgtc tcaggtttcc cgtcaactgc ctatcctatt
      121 gagattctga ttatgagatg cgttttgaca ttcgtcaaga gaaagttcca tgcttgctga
      181 ggcagtcgtg ggagtccact gaagaccgcc tatggatatc aattacgcac ccaaagcaaa
      241 aagaactcgg cagcttgacg ctgccgagtt ccttgtctcc catgtgaaaa gggtcacgag
      301 ctagcctgtc ccggaggtgg gacaacacca agctgctgta acaaccccag catatccagg
      361 ttgagccagt tttccaccat cttgccattg gcgatgcgtg ggatctgaat ccccgtcatc
      421 gtcacctgct tgcccgttgg gggtatcccc ataaacccgc ccttgtgggt cccgcgcacg
      481 gtaaagcgtg atgcaacctt gtccccttcg gcgatcagat cctcagccgt gatgtgaaag
      541 tcagggaagg ctgccaggaa catgccgatg agttgtttgg ttccctcgct accggctggc
      601 agaccaggag gcagatcgtg ctcgatgatc tgggggtcgc agaattcgcc gacggcagcc
      661 agcttcttct ggttgaagac ttcctcatac caacggcgca cgagggcctt gttttcttct
      721 gttgacatga aacgttctcc tttcgagtga tgggagttgt ggtcccgcat caccttactg
      781 atgtttgaat aggcgactac ctcttgtagt ctgtgagaag catactcagt ttgggaagga
      841 agtgcatagg gtgttcgccc tatttgtgtc atcattccac gggggcgagc agatcatgtt
      901 ccgtggcgaa gaggctagct cccgcgcggg tggagacacc gatcttgttg tagatgtgct
      961 gaatgtggtt gtccacggtc ttctctgaaa tgatgagctg accggcgacc agctttttgg
     1021 agtgaccacg cgcgatcaaa cgcaggactt caagctcgcg ctcgctcagc cctgccacta
     1081 attcgcggcg tgtgctgcgt accgagtgac ctgcagccgc gagcacacta gttgttgcct
     1141 cactatcgag ccttcccgcg cgtgcttcgc ggcgcaactg ttcggcagcg ctttcgggtg
     1201 tgagggccgg gcggtgcggg cgtggctcgg tcatagcgtg atagacatcg gccgccgcca
     1261 ggatgcgggc ggcaggtgag agcatgcttg caggcagtcc acgatagtag cccgagccgt
     1321 cgagccgctc atgatgaagg gctgccaatg ctcccaatcg cgcgagcatt tctggccggg
     1381 ccagcactcg ctcggtgtag tagggatgca tgcgcacacg ctcccactcc ctctccgaga
     1441 gtggccccgg cttcccccag atgcctgctg agacggccac ccgtcccaca tcgtggagca
     1501 accctgcccg cctgagagcc accatatcgg cctccggcag tccacagcgg cgagcagctt
     1561 gagccacgag ttcggccacg ccaactgaat ggcccagggt gtaaggcgac ttgatgtcgg
     1621 cgaaatcagc gatggcccgg caggcatgat cgaactgctc atccgagagg cgggtgcgca
     1681 caccaggctc gagcgagagg accacctccc aggatggttc ttgctcaagc cctgccagca
     1741 actgcgatgc ctgctggcag aaacactcga ccatgcgggg gtcatagctc gttcccttcc
     1801 gttgccgcgc catcgtcact gctgcctcgg caccttccag gcgatggaag gtgatggcgt
     1861 cttgcgccag tgccacgatg cgcatagaaa gggcgatgtt ctctcccttg actttgccag
     1921 gaatgcctcg accatcccag cgctcgaaga cctgtccgag ggcaagcttg atgccttcgc
     1981 cgaaaccgag tcgctcggcg aggcgttgag cgacttcaca aaatcccgtg aatccttcct
     2041 tcatcaggtg tggcgtggtc aagagtccct gagcgatcat acgggcaagg tggagcgctg
     2101 accttccctc attggcttgc tgaatggagc ggatcacgag gccgaccacc tgggatgtat
     2161 gcccactatc gacagcagcg aagtcgcctc ggagagcgag ttcatctcca aacaggtctg
     2221 ccattcggta ggtttcagca ttgcagccga tatggcgtaa aagcgccagg taatagacct
     2281 cgcgcagttc gctctcgctg agcgcgagcg cttctccaag tcgaacagac agcacacaaa
     2341 cggagagagc gtattccagc ggctgtccca tgcccaggtc agtggcaagc gagagggcgg
     2401 ccattaactc cgcaaggcga atgccagatg tggctggttg attctgttgc atatgcgtcc
     2461 ttacaagtac cccttgggaa gaagtgtagc tctcatcctt aaaaccacct cctctaaatc
     2521 accccggcac gaagaaaaag accacgcagg ccatcacata aatcgccacg agctgcaccc
     2581 ccgccagcca ggtcgattcc ccatccaggc tgatgaaggc gaacagcacg gttgccaggg
     2641 ccagcaccac cagttcgaga ggttgaaaga ccagcgtgag cggcgtggca aagaagagac
     2701 tcaccagcac gagcagaggg gcgacgaagg ttgccacttg aatggaggaa ccggcagcaa
     2761 tcgccatgct gagatcaacg cggtcgcgca gggcaatata gatagcgctg gaatgttcgg
     2821 cagcgttgcc aacaatgggg atgataacca ggccgatgaa gacgggggac cagccgagca
     2881 gcctggtgac aggtgtaatg gccccaacca gcagctcgct attccaggcc gtgccaaccg
     2941 tcgccaccag caacatcagg ccgatgaccc agggtttcgg cggcgactgg cgtatcgctt
     3001 gcccgcgcgg atttactcca cctgaggcgg cctcttcctc gccctcctct tcttctttgg
     3061 gaggcggaag cggcgccgtc ttgcccccat gtttggggtt acggcgcgcg gcccgcacgt
     3121 gaaagatgtg cagcgccagg tacaacacat acacaatcag tagcagcgcc gagataacca
     3181 cactgactgc ttgaatgcgc gcggcattgc ctatcgccag ggagccgacg gtcgggatca
     3241 gcaggccggc caccgccatc accatcatag cggtgaattc tccggcatcg cgcgcatcaa
     3301 aatattgtct gccccagcgc cagccgccga gtgcgagcga agaacccagc accagcagcg
     3361 cattgctaat caccgaacca gcgatcgacg cctttaccac cgatatcagc cccgcgcgca
     3421 gtgcaaaaat accgatgatg atttcgggcg cattcccgaa agtggcattg agcaagcctc
     3481 cggcgatggg gccaacatag tactccaggt cctcggttgc gcgcccaatc agcccggcca
     3541 gggggatcag cgcaatggcc gacgtgatga atatgaccag ttccagttgc gtcccccaga
     3601 taatctccag aattggcgca actacggaca tcacaagcag gtagtacagc cagcgaggca
     3661 tagatctact ctacggattt ctcgtcaatc acattcacgg tgatcttcgg ctcaagcttt
     3721 gtggcgatct tcaccggtac ctggtacgtc cccatcgacc ggatagggtc gggcaaatag
     3781 attgagcgcc ggtcgacgac gatgttctcg ccgtcgcgca ggccagccgc gatatcctgg
     3841 ctggtgatcg aaccgtagag acgaccttgc ttgcctacac gcgccttgaa cgtcaggctc
     3901 acactgctca gtcgctcggc ctgttgtcga tcagcctcgg cctgcttctc ctgcttgcgc
     3961 ttctcagtag caacacgctg ctggtggttc gccagcagag aaggggtcgc cggagcagcc
     4021 agtcgcttcg gcatcagata attgcgcgca aacccattgg ccacttcttt caggtcgcct
     4081 gtcttgccaa gattatcgac atcttgtaaa agaattactt tcattcctca ttcactcctc
     4141 gtcggggatg caaccgccag tacttcaatt cgcccggtca tatcccgcct gttctttttt
     4201 ggtacgctgc caagtataca ccttgtacgg aacgagcgca agcatcacaa accccgtagg
     4261 agcacggtaa accggctcct acggggtttg ggtggctgcc caacacccta tccttacgat
     4321 atcagccggt ctaatagagg tatgaccaca tcgtcccgcc tgctcgacag cgtctcacgc
     4381 aagcgttcgg gcgcgacaag gcgaatgcgg cgctggtgtt gttcgtagcg ggccaggtcg
     4441 tcgggctgca gtccggttgc ctccacccag gtcacgagcg gctccatccc aacgggcgga
     4501 tgcgcgaacg tcacccacac atccatttcc catttttccg ggcgcgggat atcgaataag
     4561 acctcgtaat ctgctatatc ggtggcaagc gtggtggcaa gctcagccgc gagctgctgc
     4621 tcaacctggc ggcggcgcgg cgcattccag aaaagcgcgt ccaaccgggc atacagacgc
     4681 cctgccaggc gactgacttc cagcacgccc ttgtatgggc ggcgcatctc cagctcttct
     4741 gccagcgcgc gcgcgctggc aggcatctcc gcgcgcgcca gcagcgccag cagcgaggca
     4801 tcgtccatgt cgatcaggcg ctcggcatcc aacgcgctac acagcagcgc ctcctgcact
     4861 gcgcgcacca gcatgacctg catcgcgcgg ctggtatgat gccagtagat attgtcgaac
     4921 atctcctggc gcgcgtggag cagcgaatgc agcgggctga tacccttgtg cgtcactacc
     4981 acgcgccgct gcccattcgc gtcgggatgg atacgcagcg cgccctgcaa acgcgccacg
     5041 tccacgccgc cgtaaggaac gttgcaggcg cgggcatcgc gtggcagata atcgagctta
     5101 tccacgtcca gcgcgccgct cagcagctcg ccgagcagca catcgtcggc aggcagaggc
     5161 tgtgaccgtg gaggatcgat gaaatccgcg acccgtccgg gcgacaggcc ataatcgcgt
     5221 tccaggatgg tcgctatctc gctcttctcg atgatggaac gccccacctt ttcgtgcagc
     5281 acgatgggat atcccagctc ctcgatcgta tgcgagaagg gataatgccc gatatcgtgc
     5341 agcagcgctg ccaccaccag cgtctgcaca ctgctgatcg aaacgccaaa cagcccgccg
     5401 ttgctgccgc gctgcagcag agcgcgtaac gcgcgcaccg ccaggtagta actccccaga
     5461 ctatgctcat agcgcgtatg cgtcgcgccc ggccagacgc gatagacgaa accgagctgc
     5521 ttgaccttct gtagacgtag gaagggagcc gtgccaatca acttttcctc gcgcaaaccc
     5581 agcggaatca aatcgtacag actatcacga acagtactaa tgggctgttc gtctggaagc
     5641 aatggatatc ccgcttcatc cacccattct cgccacattc ccgcctgtac gactgcttca
     5701 tgtgcgcgct cggctttgtt gcgaggccgt ttcacgcgcg ttcgaccggg tgattcactc
     5761 tgcctgttca tgtctgaatt ctatcagaag actaggaaga atgtctagct gttcgttttt
     5821 ggagtgattg acttctatct tctacgttgt acacaccaga ggggaagcaa agagctaagt
     5881 gaaatctgtt cgacacacag ctttctaaat tgatacattc gctcacttag ttctgaacgc
     5941 atcacacaac acctggccaa aattacggcc atcgtcgtag ggacgtgatt catcgcgttc
     6001 tggtatcccg ggctgcttta tgttctggca acgtcaggcc atattgtgtt ccagggcgtg
     6061 attcatcgtg tcccgacgcg atttaagcgt tatcacaccg tgctgtgatg cgttttgtta
     6121 ctgtttattg tatgttgcaa gtattcgtac aatggagttg aagtatagta tgaaactgaa
     6181 caacttctgg ggaactacac gatcgcccgg tttacaggtg ggcatgatcg tgcgcctggg
     6241 actcgcgctc accgccatcg cgggcagcat cgcgctggcc tgggtcttgc tcggcggtca
     6301 ttatgacgct cacgccggag ccattaatac tttaggaacg gcggatcagc caatggggct
     6361 ggtcatcgat aaaaacggca acgcctgggt cgcggaaccg aactgcaacc cggcgccggt
     6421 ctgctccgcc ccggccgcgg gcgcaattga agaattcaag ctgaatggca ccagtggtat
     6481 ctcccatgtc gccacctatc ttgctccaca actatataac ccgacctttt tgacagtgga
     6541 cgcgagcggc aatatctggt ttaccgatcc aacgaacaac gcaatcggca agctggtccc
     6601 gggggcccac cccacgtggg caaagtacac caccggcctc tccgccagtg cggagccgtt
     6661 cggcatcatc accgacaaaa acggcaagat ctggttcgcc gaacgcggca tcagcaaaat
     6721 cggtttcatc gacccgactg ccgggacgcc gaccatccat gaaaatggtg ggtttaccac
     6781 aaacagccag ccgttcggct tgaccatgga taacaccggc aacatctggt tcaccgaaga
     6841 taccgtcgcg aatctcggca ccttcacccc gaccacgaac ggcacggtca caattcatga
     6901 acatcctacc ggcacaggcg ccaacctgct gcccgcgcat atgatttcgg ctgacaagac
     6961 cgggaacctg tggtatagcg aaggcggcac cgacctggtc ggcgaatata ccatcagcgg
     7021 gggcgcaaca aa
//
LOCUS       DASZAK010000322         2667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_434816, whole genome shotgun sequence.
ACCESSION   DASZAK010000322 DASZAK010000000
VERSION     DASZAK010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434816"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1353
                     /locus_tag="VGT44_05725"
     CDS             319..1353
                     /locus_tag="VGT44_05725"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEV2580333.1"
                     /translation="MKSIVSVGEALIDLIPDKQVRLGEACFSPYPGGAIANVSVAIAR
                     LGGSSKFIGGVSDDEFGQLLVQTLAGNQVDTQYIQVIQNAPTAIALVTLQAEGQRRFT
                     FFRQETADSKLLAEHLDWIAWHDAAVCHVGGVLLSTEPARTATFAAMDYTRRVGSIVS
                     LDVNVRQSLWASNSEIRDTLAKAIVRTDILKLSAEEAEFVSEQSNPPGDLYERSWLNA
                     LGDALLEKGPRLVIITLGAQGALLLTANHRVEVPAWQVRAVDTTGAGDAFMGATLHTL
                     VQQGCSTPSDLLPLSEHDLRNLGNFAVFVAGLSVTSHGGISSFPFIYELTDFVTSQES
                     DKEQSGDKQG"
     gene            1662..>2667
                     /locus_tag="VGT44_05730"
     CDS             1662..>2667
                     /locus_tag="VGT44_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2580334.1"
                     /translation="MSRFFKTPFTLSTVAAIALVALIIGVLAGRLFAPTSTSANANAP
                     ATSNKVDVIVKATDSQFWQAMLAGAQAAGKDWGVQVGLFGATSEADVSAQVGLVENSM
                     SRNVKAIVLAASSSQALNGVIDRARGQGIKVITVDTKVTTSSDGFIGTDNSKAGAQAA
                     DRLGTLLQKKGVTSGQVLIESSVAGVQTIVDRDAGFINELKTSYPNLQVIAHRYNNND
                     TPTALSQVNDAISANPGLVGIYADNNVSADGAAQAIQENKAQSRIVAVGFDSDPQEIS
                     ALKNGTLQAIVVQNPYFFGYQGVVAAAMSAQGRFAPPSLDPGAVLVALDNMNTPAVAK
                     L"
BASE COUNT          651 a          686 c          699 g          631 t
ORIGIN      
        1 ccagacagat ccttcgctgc ggctcaggat gacaaggcca tcgagacaga tccttcgctg
       61 cggctcagga tgacaagata gggtcatggc gattccatat ccagcatgcc accacttcaa
      121 atttaaacga aacaagtctg aatttcgtgt tactagtaga gatgagaact atttgcttga
      181 aaagggcttg acaattttat tgtaatcgtt tacaattgct ttatcaaaga cgatccttat
      241 catcacagaa gttgaaacgt gctggtatag cgttgacgaa gaggcaactt gatcacagag
      301 aaacagtgag gtaacacatt gaaaagcatt gttagtgtag gtgaagcgct catcgatctt
      361 attccagaca agcaggtgcg cctgggagag gcttgtttct caccctaccc tggtggagca
      421 atagcgaatg tttctgtagc cattgcacga ctcggaggtt cttctaaatt tattggtgga
      481 gtatccgatg acgagtttgg gcaactcctg gtacagaccc tcgccggtaa tcaggttgac
      541 acgcagtaca ttcaggtaat acaaaacgcc cctactgcaa tagcactcgt aacgttacaa
      601 gctgaggggc aaaggcgttt tacctttttt cgccaagaga ctgctgatag caaattactg
      661 gctgagcacc tggattggat tgcgtggcac gatgcagcag tctgtcacgt gggaggcgtc
      721 ttgctctcca ccgagcctgc tcgcacggca acattcgcgg caatggacta tacgcgccgg
      781 gtcgggtcca tcgtatcgtt ggatgtgaat gttcgccagt cattatgggc gtccaactcc
      841 gaaattcgag atacgctagc caaagctata gtgcgcactg acattctcaa gttgagcgcc
      901 gaagaggcag agttcgtcag tgagcaaagc aatccaccag gtgatcttta cgaaagaagc
      961 tggctaaatg cgttgggaga tgcacttctc gaaaaagggc cacgcctggt gatcattacc
     1021 ttaggagccc agggagcgct tttactgaca gccaaccacc gggtagaagt gcctgcctgg
     1081 caggttcgcg ccgtcgacac gacaggagca ggagatgcat ttatgggagc taccttgcat
     1141 acattagtgc agcaaggctg ttctacacct tctgatcttc tccctctctc agaacacgac
     1201 ctgagaaatc tgggcaattt cgcggttttt gttgccggac ttagcgttac cagccacgga
     1261 ggtatttcat cgttcccttt catatacgag ctcactgatt tcgtaacctc tcaagagtcg
     1321 gataaggagc aatcgggcga taagcaggga tagcatccca ctcagggcgc ggaatttcca
     1381 gatatgatgc gcattaacaa atgaagtggg acatcagaac tcccactaag ggccgataca
     1441 tcggcgatgg gcgcgataaa tcggccccta caggtggtcg atttattttg atcgtatgca
     1501 tcatataccg taaatcatct actttttttc ctctgtatgt tgtctccgta aaacttcctt
     1561 ttactaacga gcctcttgct gaaggctcgc gtgtcccctc gatcagttgg atgcaacaac
     1621 tatgatgcat gcacacggcc tactatgaaa aggagtaagc tatgtctcga ttcttcaaaa
     1681 caccttttac gctttctact gtcgctgcaa tcgccctggt tgccttgatc attggagtcc
     1741 tggccggtcg cttattcgcc ccaacttcga ccagtgccaa tgcgaacgcc cctgccacga
     1801 gcaacaaggt cgatgtcatc gtcaaggcga ctgactcgca gttctggcag gctatgctcg
     1861 ctggcgccca ggccgcgggt aaagactggg gcgtccaggt aggcctgttc ggggcaacct
     1921 cggaagcaga cgtctccgcc caggtgggcc tggttgaaaa ttcgatgtcg cgcaatgtca
     1981 aggctattgt gcttgctgcc tcctcctcgc aagcgctcaa tggagtgatc gatagggctc
     2041 gcggccaggg catcaaagtg atcaccgtcg atacaaaggt caccacctca agcgacggat
     2101 ttatcggaac tgacaattcc aaagcaggcg cgcaagctgc tgaccggctg ggaacactcc
     2161 tgcaaaagaa gggcgtcacg tccgggcagg tactgattga atcttccgtc gccggtgtcc
     2221 agacaattgt cgaccgggac gcggggttta tcaatgagtt gaaaacgagc tatccgaacc
     2281 tgcaggttat cgcgcatcgc tacaacaaca atgacacgcc gaccgcgctc tcccaggtga
     2341 atgatgcgat ctccgccaat cctggcctgg ttggaattta tgccgacaat aacgtgtcgg
     2401 ctgacggtgc agcccaggcc attcaggaga acaaagctca gagtcgcatt gtcgcggtag
     2461 ggttcgattc tgatccccaa gagatcagcg cattgaagaa tggtacgctg caagccattg
     2521 tagtgcagaa tccctacttc tttggctacc agggtgtagt tgcagctgca atgagcgccc
     2581 agggacgatt tgccccaccg agcctggatc cgggtgcggt cctggtagcg ttggacaata
     2641 tgaacacccc tgccgtcgcg aagctgt
//
LOCUS       DASZAK010000323         4696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_221376, whole genome shotgun sequence.
ACCESSION   DASZAK010000323 DASZAK010000000
VERSION     DASZAK010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221376"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VGT44_05735"
     CDS             <1..863
                     /locus_tag="VGT44_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2580335.1"
                     /translation="ILGHEVVGVVVARGSEVTHPEIGQRVVPGAGWWCGECPRCKEGR
                     INICENYYLYGIHANGGLAEFARFPAQMCMPVPPECSDEAAAMAQPCAVALHALARGG
                     IAIDQTVALFGVGSIGNLLLAALQAQAAGGQRVIAIDVEQGRLATARRLGAFSQINAR
                     ADDPVAAIGELTGGRGVDVAIDATGVPETIAQALASVRRGGRLLQVGIPVAPMRLVLD
                     TAIVQEKDIITTNGQVCGIDLPNALELLSTTDLAQRISYRVISLDALVEEGLVPLVEH
                     RATTKVVVAI"
     gene            900..1775
                     /locus_tag="VGT44_05740"
     CDS             900..1775
                     /locus_tag="VGT44_05740"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEV2580336.1"
                     /translation="MIRTIELIASYWTIAGDVYPFAPNEISPFPFEKRVEVAARAGYR
                     GLGLLHADLMAVSNRLGYSEMRRILDANGIYHVELEFLSDWYAQGRARAESDMVRKDL
                     LEAAEALSARCIKIAPSIDEPSIDDVALDMPRMIESFAALSREAAKYGTSIALEIMPF
                     SNIRTLSAALELVSTDPQPNGGLYLDIWHMARGGIDYSEVAKIPQQFIKAVELDDADR
                     DVVGTLWDDTRFHRRLCGEGALDIPAFLKATREAGYRGPYAVEIISQEHRRLSLEEEA
                     RRSFETTVAQFRNLD"
     gene            1912..2718
                     /locus_tag="VGT44_05745"
     CDS             1912..2718
                     /locus_tag="VGT44_05745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2580337.1"
                     /translation="MTMKELTGKVVCITGAAGGIGSAIARAFAGAGTNLALLDLNEEG
                     LSRLANELRGIRIPPATVSTAVADLSTEQGVKSGIAEVLSAFGNRIDILVANVGFLIA
                     QRFEELTAEQWQRGFALNFFTHVYACQAVLPLMKQQGGGSIIFTGSDQGLQPDAGLGP
                     YAQAKAAVHSLVKMLARELPSDGILVSAVAPGMTRTPLVERLMEGYAREFGTDSKTAE
                     KLELQRRGVPLGRLGEPEEVAEAVLYLATAAYCNGTILNISGGNVRSVTC"
     gene            2805..3866
                     /gene="iolG"
                     /locus_tag="VGT44_05750"
     CDS             2805..3866
                     /gene="iolG"
                     /locus_tag="VGT44_05750"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEV2580338.1"
                     /translation="MSAPLKIGVIGLGRMGQLYARTLMTQVAGVQLYAVGESDEQLRI
                     TTAREFNVLHAFADAHELLSLSELQAVVIATPTQTHAELVIAAARAGKAIFCEKPLAL
                     TLDATHRALDVVAEAQVPLQVGFMRRFDGAYKKAKALIDAGNIGRPVTFKSVGRDPFC
                     PRPDYADPSQSGGLILDMAIHDFDLARWLMGSEVERVSAEGTLMVCEQLQRVGDIDNA
                     VVNLRFVSGAVGNVEVSRNAFYGYDIRTEVLGSEGAVIIGAHQHTPVVLLTRNGAQHD
                     TVPYLMERFGDAYRAQIQHFADCLQAGKPPSVGGPDALAALEIGIAATRAYQTSRIIT
                     LKAGRRAAKTLREHLGPRQ"
     gene            complement(3934..4494)
                     /locus_tag="VGT44_05755"
     CDS             complement(3934..4494)
                     /locus_tag="VGT44_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580339.1"
                     /translation="MSINAQLHSAFFYLVLVYALTGSGAGLFIPYFNIYFVQHLRASP
                     ALFGLIDGGANAITALLTLTAPWFARRIGKINTITLTRLASIPLLLTIGLTPFLPLAA
                     LLYLFRQGTMDMSAGILQVFSMEVVSEKHRGLANSSYQSAFQVPWAITAPIGGLLIVH
                     FGYPPIFLLGALFYILTIVTLWAKFR"
BASE COUNT         1058 a         1230 c         1418 g          990 t
ORIGIN      
        1 tcatcctcgg acacgaggtg gtgggggtcg ttgtggcgag aggatcggaa gtgacccacc
       61 ctgagatcgg gcagcgtgtc gttcccggcg ctggatggtg gtgcggggag tgccccaggt
      121 gtaaagaagg ccgcatcaac atctgcgaaa actactacct ctacggtatt catgctaacg
      181 ggggccttgc cgaattcgcc aggttcccgg cgcagatgtg catgcccgtt cccccggaat
      241 gtagcgatga agccgcagcg atggcgcagc cgtgcgctgt ggctctgcac gccctggcaa
      301 ggggtggaat cgccattgac cagacggtcg cgctcttcgg cgtcggcagc atcgggaatt
      361 tactcctggc cgccttacag gctcaggctg cgggtggaca gcgagtcatc gccatcgatg
      421 ttgagcaggg caggctcgct acggccagac ggttgggcgc gttctcccag atcaacgccc
      481 gtgcggatga tccagttgcc gccattggcg agttgaccgg tgggcgagga gtcgatgtcg
      541 ccatcgatgc gaccggtgtt cccgagacga tcgcgcaggc acttgcctcg gtacgtagag
      601 gcgggcggct cctccaggtc ggcattcctg tggcccccat gaggctggtg ctggatacgg
      661 cgatcgtgca ggagaaggat atcatcacga cgaatggcca ggtatgcggt atcgacttgc
      721 ccaacgcact ggagctgctc tctacaacgg atctcgccca gcgcatcagc tatcgcgtca
      781 tcagcctcga cgcgctggtg gaagaggggc ttgtgccact ggtagaacac agggcgacca
      841 cgaaagtcgt ggttgctatt taatatggga ggacgctgcg tccttaaagg agaccacctt
      901 tgatacgcac cattgaactc attgcctctt actggactat cgcgggagac gtgtatccgt
      961 ttgccccaaa cgaaatcagc ccgtttcctt tcgaaaagcg ggtcgaggtt gcagccagag
     1021 ctggttatcg tggcctgggt ttgctgcatg ccgacctcat ggcggtttcg aaccgtcttg
     1081 gctactcgga gatgagacga attttggatg ccaacggcat ttaccatgtt gaactcgaat
     1141 ttctgtccga ctggtacgcc caggggcggg cccgtgccga atccgacatg gttcgcaaag
     1201 atttgcttga ggcggctgag gcgctttcgg cacgctgcat aaaaatagct ccaagcattg
     1261 atgaaccctc gatcgacgac gtggctctcg atatgccgcg aatgattgag tcctttgcgg
     1321 cactctcacg agaagcggcg aaatacggga cctcgattgc gctcgaaatc atgccgttca
     1381 gcaacatccg aaccctttcc gcggcattgg aactcgttag cactgatcca caaccaaacg
     1441 ggggtctcta tctagatatt tggcacatgg cacgcggagg gattgattac tccgaagttg
     1501 ctaagattcc tcagcaattt atcaaggcgg tcgagctaga tgatgccgac agagatgtgg
     1561 ttggaacact gtgggacgac acgcgcttcc atcgtcgcct gtgtggcgaa ggtgccctcg
     1621 acataccggc gttcctgaag gccactcgcg aggcgggata ccggggccct tacgccgtcg
     1681 agattatctc gcaggagcat cgtcgcctct cccttgagga agaagccagg cgctcattcg
     1741 agaccaccgt ggctcaattc cgcaacctgg attgatgact acatctccag aaaagaaata
     1801 ccgttatgtc ggaatcaacc aatcgatgaa ctagcttgaa cgttgcgtgc atggatggac
     1861 ctgttatgac ctacaaagtg tctttcagaa caagcgaaaa ggagcagagc aatgactatg
     1921 aaggaactga ctggaaaggt ggtgtgcatc accggtgcag ccggtggcat cggctcggcg
     1981 atcgcacggg catttgccgg ggcgggcacc aatctcgcgt tgcttgatct caacgaggaa
     2041 gggctttctc gcctggcgaa tgaattgcgt gggattcgaa taccccctgc cacagtgagt
     2101 acagcagtgg cagacttgag taccgaacag ggggtcaaat ccggtatcgc agaggtcctc
     2161 tccgctttcg ggaatcgcat cgatatcctg gtggccaacg tgggcttcct cattgcgcag
     2221 aggtttgagg agctcacagc agaacagtgg caacgcggat tcgcgctcaa cttctttaca
     2281 cacgtgtacg cttgccaggc agttcttccc ttgatgaagc agcaaggagg aggctcgatc
     2341 atctttaccg ggtcggatca gggtctgcaa cctgatgctg gattggggcc ctatgctcag
     2401 gcaaaggccg cagtgcatag cctggtcaag atgctcgcca gggaattgcc ctccgatggc
     2461 atcctggtca gcgcggtcgc cccgggcatg acgcgaacgc cgctggtgga aaggctgatg
     2521 gaggggtacg cgcgggaatt cgggacggat agcaagactg cagagaagct agaattgcaa
     2581 cgtcgcggtg ttccgctggg caggttgggg gagcctgaag aggtggcaga agcggtgctc
     2641 tacctggcaa cggcggcata ctgcaatgga acgatattga atatcagcgg ggggaacgtg
     2701 cgttcagtga catgctagac agagggggtc tccctaagtg gcgaccccct actcatcgtt
     2761 tccgtcgtcg tagtatcaat agagaagaaa ggatgtctgt atctatgtct gccccgctta
     2821 agataggagt tattggactc gggcgtatgg gtcagttgta tgcacgcact ttgatgacgc
     2881 aggttgcggg agtgcagctt tacgcagtgg gagagtccga tgaacaactg agaatcacca
     2941 cggctcgtga gttcaacgtg ctacacgcat tcgccgacgc acacgaactt ctctcacttt
     3001 ctgaattgca ggcagtggta atcgccacac ccactcagac acacgctgaa ctggtcatag
     3061 ccgctgccag ggcgggtaag gctatttttt gcgaaaaacc actggccctg acgcttgacg
     3121 cgacccatcg cgcgctcgat gttgtcgctg aggctcaagt accgctccag gtcggtttca
     3181 tgcgccgttt tgatggagca tataagaagg caaaggcact gatcgacgct ggaaacatcg
     3241 ggcgtcctgt tacctttaag tctgtcggac gcgatccgtt ctgcccacga ccggactatg
     3301 ccgacccgtc acagagtggt ggcttgattc ttgacatggc gattcacgat ttcgatctcg
     3361 cacgctggct tatgggcagc gaggtcgaac gggtctcagc ggagggaacg ctgatggtat
     3421 gcgagcagct tcagcgcgtc ggagatatcg ataacgccgt ggtgaatctg cgcttcgtga
     3481 gtggcgcggt gggcaacgtg gaagtgagcc gcaacgcctt ctatggctat gatattcgca
     3541 ccgaagtgct tggctccgag ggggccgtta taattggcgc tcaccagcat acacctgttg
     3601 tcttgctcac acgcaatggt gctcaacacg atactgtgcc gtacctgatg gaacgcttcg
     3661 gagacgccta ccgcgctcaa atacagcact ttgctgactg tttacaggca ggcaaaccgc
     3721 catcagttgg gggcccagat gcgctagcgg ctcttgaaat aggtattgct gctacacgag
     3781 catatcaaac gagccgcatc atcacactta aggccgggag acgcgcggcg aagacattac
     3841 gcgagcattt gggaccacgg cagtagaaat tgcgaacgat gtgcaagcag tgtgccccta
     3901 aagcggccat aagccagtac attatcaaat gtatcatctg aatttagccc acagagtaac
     3961 aattgtcaat atgtaaaaca atgcgcccag gagaaagatg ggtggatagc cgaaatggac
     4021 gataagcaac cctccgatcg gggcagtgat cgcccaggga acctggaatg cagactggta
     4081 actgctattt gccagccccc tgtgcttctc tgacaccact tccatggaga agacctgcag
     4141 gatgcctgct gacatgtcca tcgtgccctg gcgaaaaagg tagagcagtg ctgcgagtgg
     4201 caggaatggg gtcagtccaa tggtgagtaa gagcggaata ctggcaagcc gagtcaaggt
     4261 aatcgtgttg attttgccga tgcgcctggc aaaccagggc gcggttaacg tgagcagagc
     4321 ggtgatggca tttgctccac cgtcgatcag gccaaaaagc gccggagaag cgcgcagatg
     4381 ctgcacgaag taaatgttga agtatggaat gaataggcca gctcctgatc cggtgagcgc
     4441 gtaaaccagc accagataaa aaaatgcgct atgtagttgt gcgttgatgc tcacccatat
     4501 ctgcttccag gagatcctgt gcaagggcaa ccactctcga ggagattgta aggggagagt
     4561 tgtttccgag atgttttgag cagccgaaac aggcacagga tgatgctgag aattgtcctc
     4621 agataccgga cgaagcaatc gcccctgcag ctcgtcctca tccctacccc tacgcggttg
     4681 gcgacgggga gggcga
//
LOCUS       DASZAK010000324         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_327883, whole genome shotgun sequence.
ACCESSION   DASZAK010000324 DASZAK010000000
VERSION     DASZAK010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327883"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1294
                     /locus_tag="VGT44_05760"
     CDS             <1..1294
                     /locus_tag="VGT44_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV2580340.1"
                     /translation="VMGRIEEELSRCRRAKEICALLFIDLDHFKRVNDTWGHRAGDAV
                     LVEAGRRLSSVIRLEDFAGRYCGEEFAVLLVNVDLQAARAVGERLREALEASPCQWQA
                     DDTGQVVSIQMSGSIGVAVFPEHSQNSAALIEAADAAMYQAKRGGRNRVCCAGDALSL
                     AQEVLSDERLVEDKMALQALLATIQAFDQDTGSHAIRMMQLAVETATMLGCSDEEQHL
                     LRLAALMHDIGKIGIPQEILHKPGPLTDEEWEIMRRHPSIGRQILAQAGGQCALLSHI
                     VIAHHEHWNGKGYPYGLAHDAIPLGARILSVVDSYDAMTSDRPYRSAMPVEDAIAELE
                     RCAGAQFDPQVVSAFLLTLEQREQETILRQEERREIKSDALRNFGDEGGPVAADGVLR
                     GDPDVVAGEAGIGSGLEVAPTQVVGGHRPLDDGVGVPH"
BASE COUNT          660 a          837 c         1032 g          649 t
ORIGIN      
        1 ggtgatgggc cgcatcgaag aagaactttc gcgctgccgg cgagccaaag aaatctgcgc
       61 gttgttgttt atcgacctcg atcacttcaa gcgcgtcaac gatacctggg gccaccgcgc
      121 gggcgacgcg gtgcttgtgg aggctgggcg gcgcttatcg agcgttatac gtctcgaaga
      181 ttttgccggg agatattgcg gcgaagaatt cgccgtcctg ctggtgaatg ttgacctgca
      241 agccgccaga gccgttgggg agcgcttgcg cgaggcgctg gaggcatcgc cctgccagtg
      301 gcaggcggat gataccgggc aggttgtgtc gattcagatg tcgggcagta ttggcgtggc
      361 cgtcttcccg gaacatagcc agaacagcgc ggccctgatc gaagcggcgg atgcggcgat
      421 gtaccaggcg aagcgcggcg gacgcaatcg tgtctgctgc gccggtgatg ccctgtccct
      481 ggcgcaggaa gtgctgagcg acgaacggct ggtggaagac aaaatggcac tgcaagcgct
      541 gctggccacc atccaggcgt ttgaccagga taccggctca cacgctatcc gcatgatgca
      601 gctggctgtt gagactgcaa cgatgctggg ctgctccgac gaggaacagc acttgctgcg
      661 cctcgcggca ctgatgcacg atatcgggaa aattggcata ccacaggaga ttctgcacaa
      721 gccgggaccg ctcaccgacg aagagtggga gattatgcga cgccatccta gcattggccg
      781 ccagattctg gcgcaggctg gcggacagtg cgcgctgctt tcacacatcg tcatagcgca
      841 ccacgaacac tggaacggca agggctatcc ctacggattg gcgcacgatg cgatccccct
      901 gggcgcgcgt atcctctcgg tggtggactc ctacgatgcc atgacctccg accgccccta
      961 tagaagcgct atgccggttg aggatgccat cgccgaattg gagcgctgcg cgggcgctca
     1021 gttcgatccg caggtcgtgt ctgcgttcct gctcacgctt gagcagcgcg agcaggagac
     1081 catcctccga caggaggagc gcagagaaat caaatccgat gcactacgaa actttggcga
     1141 tgaaggtggc ccagtcgccg ctgatggagt actgcgaggc gacccagacg tggttgctgg
     1201 agaagccggt ataggaagcg gcctcgaagt cgccccaacg caggttgttg gtggccacag
     1261 gccccttgat gatggagtgg gcgttcccca ttgaaccgag cggggccgac ttgctatgcg
     1321 agacgatcat gatgcctgga ttgagattgg cgctcatcgt atcgaagacc atgtacaaag
     1381 tgccattctt atcctgcatc atggcgccaa aggaggcgtc ctggtcattg gggaagtaga
     1441 ggtagccgct ctggtagaga ttggcgaaga caatgccaac gccgtgcgtt acctgaatct
     1501 gcccccacag gataccgggt acgaagttcg ggaaaatcgg cccgccgttg ctgaccgccg
     1561 tatcgaggga gaaggagatc agcccctggc cgccgctgat actgtagacg ggtgtgccgg
     1621 taatgcgatt atcgatagtt tcgatgcact ggatgcaccc cggttcatca gcgttgggcg
     1681 gtgtgatgta ggttggattg ggaataagct ggccgacgat tgtgccgacc agggtcgccg
     1741 tagcgggagt gaaggcccac accacaaatc cctggcaggt ggtgaagaag cagttcccgc
     1801 caaacgggtc gccattcatg ttgaaggagt tcaccaggta ttggacgccc ggatcgttgg
     1861 ctgccgtcgt ctccgtttca accggttgca cggtgtcaac caggacacca ttgacgctga
     1921 gggtggtaaa ggcgacgggg gtcacattct ggcattgctc catggtgtgc ttgtcggcga
     1981 attgcgtctc ggcgtaggca taagccgagc catcattcgt gaacatgttg ccggagaagg
     2041 cgatggacga gccgctaaag ccgaactggg tgtagtcggc gacgtttttg gtgccaagcg
     2101 ccatgtcgaa gtggtagacg cacatcgccc cggtattggc gttaatgttg gcaacccagt
     2161 actgcgaaag gaatttgcag cctggagcga gtcctatacc gttctcaagc tggagcagcg
     2221 ccgcccagaa gtaaccgtcg tttggatcgt agaaggcgcg cgggtcgcta gtgaaggggc
     2281 cgagggggtc gcagccgctc ggtgttggat tgggaacgcc gaaaaaggct tgctcgttga
     2341 aggggcctgc caccagcccg ccttgtgtgt tgtaaaacgc gaacgaggta ttcacgcctt
     2401 gaaaaaccat ctgcggcgag gcggcgagcg ccatatcggg aggctggcag ccgccaaagt
     2461 aggggcagat gctggccgag tcggccaggc cattgaagcc ggtcgcggat gagtgggtcg
     2521 aaccaatgct ggcactggtg gcctgtccgc cagttgggga gtttttgagg cgcgcggcct
     2581 gctgtttggc cttaatgaca ctggggctga gcagcagcgg catggcacga tggcttttcg
     2641 tggtcgatgc tgtggcggcg cgcgttatag ccaggccatt caagctcctg ggctgcccga
     2701 tggcaacggg cgcgctcacc tttttcgcgg tgatgccacc accgctgccc ccacctgaat
     2761 aagggctatt cccctttgcg aggacggcag tcgcggaaaa gatgctggct aataaggctg
     2821 ctatgacgag cagggaacag aacgcaacct tgcggtgatt ccagatcatt ctacctctcc
     2881 tatttccatc aaggcttcgt gattacctga ttgatccact ctcatcaagt aatcgacgat
     2941 ggtacacgac aaatctcagg acgactgggt gtggttgcca cgagtatgaa gcggtttgcg
     3001 tatcctcctt tctcggtagg cttcgatact ggcgagggtg gcgagggaat atcgaatggt
     3061 gatgctttcc gacgagtcag ggaaagcggg cgttctgata agcataacca tatcaagtat
     3121 agagatagca atgtattcag atcgttgtga tcgcagtaat agtatggtgt catactgt
//
LOCUS       DASZAK010000325         2256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_363662, whole genome shotgun sequence.
ACCESSION   DASZAK010000325 DASZAK010000000
VERSION     DASZAK010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363662"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..2220
                     /gene="ftsH"
                     /locus_tag="VGT44_05765"
     CDS             268..2220
                     /gene="ftsH"
                     /locus_tag="VGT44_05765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV2580341.1"
                     /translation="MDNLNQPPAGSRGQPPKPPQIKWTSLLWLLLAVLVLSWLLPSFL
                     GSGGASNEAVTYSTFVAQVTANNVRSVTITDYTVTGVFKTPVLSSDGTTKSTQFTTTV
                     PQFGNADVIALLQAHDAAITVQSSSNTTFWLNMLLVVGPLVMIFVLFWWMSRRVTSAQ
                     SGLFSFGKSKARLYLGGKTKTTFADVAGVEEAKAELAEIVEFLKAPARFQKLGGKIPR
                     GVLLVGAPGTGKTLLARAVAGEADVPFFSMSGSEFVEMLVGVGASRVRDLFDTAKKSA
                     PCIIFIDELDAVGRERGAGLGGGNDEREQTLNQILVEMDGFDPHTAMIVLAATNRPDV
                     LDPALLRPGRFDRQVVVDRPDRLGREAILRVHVRGVPLAPDVKLDVIAASTVGMVGAD
                     LANLVNEAALTAARHNLEQVTQACFEEAMDRLMLGAERPLVLSKEERRVTAYHEGGHT
                     LVALLTPEADPVHKVTIIPRGQALGATHIVPLDDRHNYPRSYLLARLAVGLGGRVAEE
                     QEIGEITTGAENDLKDVTQLARQMVTRWGMSSRLGAVFLGGEQEVFLGREVGLREREG
                     YSEQTAALIDEEVQQLIGERYRFVQQLLSQHPAELARIAQALLERETLDAQQLQQLLD
                     GDGKGAEMAHESETDSDILAAMIRPG"
BASE COUNT          432 a          681 c          745 g          398 t
ORIGIN      
        1 cgtgaggccg ccgccagcta ccaggacctg ggaggaacct ggctcgcttc cagtcgtatc
       61 gaggaggtgc ctggtgcatc gttggtcgcg gctgtcttcc gttattagcg tgcagtcctc
      121 gtagatgggg agataggaga acctctatgc aagacgatgt gttcgacaac aaaccccctg
      181 cgggccttcg ccaccgacca caaaacgggt caggaacgcg acgagaagct aagctaagct
      241 aagcaccaaa ggatggaggt cagcagtatg gataatttga atcaaccgcc tgcggggtcg
      301 cggggacaac cgccgaaacc gccacagatc aagtggacat cgctgctctg gctgctgctc
      361 gcggtcctgg tcttaagctg gttgctgccc tcattcctgg gttcaggtgg ggcgagcaat
      421 gaagcggtga cgtattcgac ctttgtggcg caggtgactg ccaacaatgt ccggtctgtg
      481 accattaccg attataccgt gacgggcgtc ttcaaaacac ccgtgctgtc ttcggatggc
      541 accaccaaat cgacccagtt cacgaccacc gtcccccagt tcggcaacgc cgacgtcatt
      601 gcgctcctgc aagcgcatga tgccgccatc accgtacaat cctccagcaa cacgacgttc
      661 tggttgaaca tgctgctcgt cgtcgggccg ctagtcatga tctttgttct gttctggtgg
      721 atgagccggc gcgtcaccag cgctcagagc ggcctcttct cctttggcaa gagcaaggcg
      781 cggctctacc tggggggcaa gacgaagacg acctttgccg atgtggcggg cgtcgaggag
      841 gccaaagccg aactggccga gattgtggag ttcctcaaag cgcctgcccg cttccagaaa
      901 ctgggcggca agatcccgcg cggcgtcctg ctggtcggcg cgcctggcac cggaaaaacc
      961 ttgctggctc gggctgtggc gggcgaagca gacgtgccct ttttctccat gtccggctcc
     1021 gagttcgtcg agatgctggt cggggtaggc gccagccggg tgcgcgacct gtttgacacg
     1081 gccaagaaaa gcgcgccatg catcatcttc atcgatgaac tcgatgccgt cgggcgcgag
     1141 cgtggcgcgg gccttggagg aggcaatgat gagcgcgaac agaccctcaa ccagatcctt
     1201 gtggagatgg acggctttga cccgcacacg gccatgattg tgctagccgc cacgaaccgc
     1261 ccggatgtgc ttgaccctgc cctgctgcgg cctggccgct ttgaccgcca ggtggtggtc
     1321 gatcgccccg atcgccttgg gcgcgaggcc atcctgcgcg tgcatgtgcg cggcgtaccg
     1381 ctggcacccg atgtgaagct ggacgtcatc gccgcctcga cggtgggcat ggtgggcgcg
     1441 gacctcgcca acctggtgaa tgaggcagcc ctcaccgccg cgcggcacaa tttggagcag
     1501 gtgacacagg cgtgtttcga ggaggccatg gaccgcctga tgctgggggc cgagcgcccg
     1561 ctggtcctct cgaaagagga gcggcgcgtg acggcctacc acgagggcgg ccacaccctc
     1621 gtggcgttgc tcaccccgga ggcggacccg gtacacaagg tcacgattat cccgcgcgga
     1681 caagcgctgg gagcaacgca catcgtgccg ctcgatgacc gccacaacta cccgcgcagc
     1741 tacctgctgg cgcggctggc cgttggtctg ggcgggcgcg tggcggagga acaggagatc
     1801 ggggagatca ccacgggggc ggagaatgat ctgaaagacg tgacgcagct cgcccggcaa
     1861 atggtgacgc gctgggggat gagcagccgc ctcggcgcgg tgttcctggg tggcgaacag
     1921 gaggtcttcc tggggcggga agtcgggttg cgcgagcggg agggctactc cgaacagacg
     1981 gccgcgctga tcgatgaaga ggtgcaacag ctcatcggcg aacgctaccg cttcgtccag
     2041 caactgctct cgcagcaccc tgcggaactg gcgcgcattg cgcaggcgct gctggaacgc
     2101 gaaacgctgg acgcgcagca actccagcag ctgctcgatg gtgacggaaa aggagcggag
     2161 atggcgcatg agagcgagac ggacagtgac atcctcgcgg caatgatccg cccaggctag
     2221 cgatgcttcc gctcatgaat aaatcaactt ctgtga
//
LOCUS       DASZAK010000326         4730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_363687, whole genome shotgun sequence.
ACCESSION   DASZAK010000326 DASZAK010000000
VERSION     DASZAK010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363687"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VGT44_05770"
     CDS             complement(<1..362)
                     /locus_tag="VGT44_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV2580342.1"
                     /translation="MQPRRQTSCLEEGQLTAWHDGVLSQAEAMSVMSHVSTCARCAAL
                     ERSLQNDRRQVFALLSSIDPLPGVTPQPVAAFAHFRERLAAQNAEHPLHPEDADHRPG
                     NVTFPALEVERNGLRPIAP"
     gene            complement(367..915)
                     /locus_tag="VGT44_05775"
     CDS             complement(367..915)
                     /locus_tag="VGT44_05775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2580343.1"
                     /translation="MSKDERESQLVTSEEVELFESIFTRYYPMVYRLAYRYVGQPDEA
                     EDIAQEVFLRYYHLPPHADNETQQRAWLCRVTINLGLNALRKRKSYVNQEWASGGFSH
                     EREWESNEEQNPERLVLVEEQSALVRDTLARLPERQQVCLMLRSAGCSYEEITEATGI
                     PITSVGTVLARAMQAFRRRYNE"
     gene            complement(1214..2338)
                     /locus_tag="VGT44_05780"
     CDS             complement(1214..2338)
                     /locus_tag="VGT44_05780"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV2580344.1"
                     /translation="MTTRTSTPFLSTFPGRYYYDQAIFELEQERVFSAMWYYACRADE
                     VPLTGSYKVVTVGKESVIVARDKEGALHAFLNVCRHRGARLCSEAAGQLKGSIQCRYH
                     AWTYGLDGRLIGAPNVMSDERFDKTRFGLMSVALHVWEGLVFLNLAENPQPFEEQLND
                     PRVQSFGDVAPYERYQIGHLKVAQSITYDVRANWKLIIENTLECYHCGPMHPELCALI
                     PLYRSGKLFENGEMLEGAALGENVEAFTMTGKASRPSLPGLLPVDTRRYYGFLLLPNV
                     FINLLSDHVAIDTLVPLAADRTLVTSAWLFDPDEMAKPTFDPSDAVGVLDLVNRQDWE
                     VCELAQQGVSSRAYARGGNYAPDEHHIRGFVEYVLEKLGH"
     gene            complement(2449..3504)
                     /locus_tag="VGT44_05785"
     CDS             complement(2449..3504)
                     /locus_tag="VGT44_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580345.1"
                     /translation="MQETSGRISAAATAGFSMPSVRQVRVHAVPAPLVTEVMQTARKQ
                     LYRFSTTNVGLMQLAVDARGTVWAGEMNVNRLGRLDSHTNAVTTWTPPGAMYGIMTTV
                     IDAQGNVWFAEQNANYIGRFAPDRQAFRLFPLGTWKGSQLGPQDLRFDAHGMLWFTAA
                     LAGAIGRLDPGTGAIHIWPIPPSTPTVPSSPTCLTIASNGLVWFGDYAGGAIGSLDPH
                     TGQVTLYDLPDSQAQVFAMAIDATGRLWFTEVLPGRLGMFDPATGVLTELTLQGKPGA
                     IPALFGLIIDHQGNIWFVDVGADMLVRYVPERRVLTFFQLSLPSSAPSGLTIDPGGNL
                     WFTAGGAPANYVGEITL"
     gene            complement(3683..4339)
                     /locus_tag="VGT44_05790"
     CDS             complement(3683..4339)
                     /locus_tag="VGT44_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580346.1"
                     /translation="MAQPQNTIPLQRGKRPLTSLAIMSVGLIFGMLLCVAISSGIYFI
                     FEAASGSGDAPTIAHAAQIERHVQANLKIVLNQPGMQNDWPAYRPNALVVPAHSLVTI
                     TIRNYDLGDTPLLAGSPFSAVQGVVGGVAYVNGRAYTSLAPEKVAHTFTIQQMGINVP
                     VPGDAAAGKTYVEVSFTFRTGEAGLYYFRCFDPCGTGAVGWQGPMVTRGYMLGTLTVQ
                     "
BASE COUNT         1089 a         1378 c         1289 g          974 t
ORIGIN      
        1 ggcgcgatgg ggcgcagtcc attccgttcc acttccaacg ccggaaaggt cacgttccct
       61 gggcgatggt ctgcatcttc cgggtgcagg ggatgttccg cattttgtgc cgcgaggcgc
      121 tcccggaaat gagcgaaggc ggcgaccggc tggggagtca cgcccggcag cgggtctatg
      181 ctggagagca gtgcaaatac ctggcggcgg tcgttttgga gggacctttc cagggcagcg
      241 cagcgagcgc aggttgacac gtgagacatc acgctcatcg cctcagcctg agagagtacg
      301 ccatcatgcc acgccgttaa ttgcccttct tccaggcagc tagtttgtcg tctgggttgc
      361 atttgttcac tcattgtatc ttctccgaaa tgcctgcata gcccgcgcca ggaccgtgcc
      421 aaccgatgtg atgggaatac cggtcgcctc cgtgatctct tcgtaactgc agcccgcgct
      481 acgcagcatc aggcagacct gctgccgctc gggcaggcga gccagggtat cgcgcaccag
      541 cgccgactgc tcctcgacca gtaccagccg ctcagggttc tgttcctcgt tgctctccca
      601 ctcgcgctcg tgcgagaacc caccgcttgc ccactcctgg ttaacatagc ttttacgttt
      661 gcgcagcgca ttcaagccca ggttgatcgt gacgcgacag agccaggcac gctgttgtgt
      721 ctcattgtca gcatgtgggg gcaggtgata atagcgcagg aagacctctt gtgcgatatc
      781 ttctgcttca tcgggttgcc ccacataacg atacgccaga cggtagacca taggatagta
      841 gcgcgtaaag atgctttcaa atagctcgac ttcctccgaa gttacgagtt ggctttccct
      901 ctcatctttt gacatatcat cgttccgctc tcacattcag gactacagca ctccgcccat
      961 ccacacctct tgcttttgca tctataagac acagctgtgt ccgtttttat gacggaattg
     1021 gtaccaaaaa gaagagggag aagaaaattc tcagtgatct gaaagcgggt caacatggaa
     1081 agcggatcag catgtcatcc ttcgctgcgc tcaggatctc gcgttgagta gtacgattct
     1141 tcatcgagcg ccgagatcct tcgctgcgct caggatgaca gcgccaatct taattgctgg
     1201 agttcattac cttttagtgt cccaacttct ccagcacata ctccacgaat ccgcgaatat
     1261 ggtgttcatc aggggcataa ttcccgccac gcgcgtaggc gcgcgaactc acgccctgct
     1321 gcgccagctc gcagacctcc caatcctgcc ggttgaccag gtcaagcacg ccgacggcat
     1381 cggacgggtc gaaggtgggt ttcgccatct cgtccgggtc gaacagccag gccgatgtca
     1441 caagcgtacg atcagcagcg agcggcacga gtgtatcgat ggccacgtga tcgctgagca
     1501 ggttgatgaa gacgttgggc agcagcagaa agccatagta gcggcgcgta tcgaccggca
     1561 gcagaccagg cagcgatgga cggctggcct tgccggtcat ggtgaacgcc tccacatttt
     1621 cgcccagcgc cgcgccttcc agcatctcgc cgttctcgaa aagcttgcca gagcgataga
     1681 gggggatcaa cgcgcagagt tctggatgca tcggcccgca gtgatagcac tccagtgtat
     1741 tttcaatgat aagtttccag ttcgcgcgca catcataggt gatgctctgc gcaactttca
     1801 gatggccaat ctggtagcgt tcgtagggcg ccacgtcgcc aaagctctgt accctgggat
     1861 cattcaattg ctcttcaaat ggctgcggat tctcggcgag gttgagaaag accagtccct
     1921 cccagacatg cagcgccacc gacatcaggc caaatctcgt cttatcgaac cgctcatcgc
     1981 tcatcacgtt gggcgcgccg atcaaccgcc catccaggcc atacgtccag gcgtgatagc
     2041 ggcactggat cgagcctttg agctgccccg ccgcctcgga acacaaacgc gcgccgcgat
     2101 gcctgcacac attcaggaag gcatgcagcg caccctcctt atcccgcgct acaatcacgc
     2161 tctccttccc aacggtcaca accttgtacg aaccagtaag cggcacttca tcggcgcgac
     2221 aggcatagta ccacatcgcg ctaaacactc gttcctgctc tagctcaaag atagcctggt
     2281 cataataata cctccctgga aacgttgaaa gaaatggcgt actggtgcgc gtcgtcatag
     2341 aaacctcgat tgatatgcaa agtcgggtat aaccagggcg accgccaggg ccgcccaaca
     2401 ggagctttct catgagcagt ataacacagg taagcgaagc gatgaccttt agagagttat
     2461 ttccccaaca taattggctg gcgccccccc ggcggtaaac cagaggttgc cgcccgggtc
     2521 gatggtgagg ccagatggag cgctggacgg caaggagagc tggaaaaacg tcaaaacccg
     2581 tctttccggc acgtagcgaa ccagcatgtc tgcgccaaca tccacaaacc agatattacc
     2641 ctggtggtca atgataagcc caaatagggc aggaatagct cctggtttcc cttgcaacgt
     2701 cagctcggtc aaaacgccgg tagccgggtc gaacataccg agcctgcccg gtaatacttc
     2761 ggtaaaccac agtcgcccgg tggcatcaat cgccatggcg aagacttgcg cctgtgagtc
     2821 tgggagatcg tagagtgtga cctggcccgt gtgcgggtca aggctgccaa tcgccccgcc
     2881 agcatagtca ccaaaccaga ccagaccgtt agacgcgatt gtgaggcagg taggtgagga
     2941 gggaaccgta ggcgtagatg gagggatggg ccatatgtgg atggcgccgg tcccggggtc
     3001 caatcgtcca atggctccgg ccagcgccgc cgtgaaccac aacatcccat gagcatcaaa
     3061 gcgcaggtct tgcggcccga gctggcttcc cttccaggta ccaagcggga agaggcgaaa
     3121 tgcctggcga tcaggagcaa agcgcccaat gtagttggcg ttttgctccg cgaaccagac
     3181 attcccctgc gcatcaatga ccgtcgtcat gattccatac atcgcgcctg gaggagtcca
     3241 ggtagtcacg gcgtttgtgt gtgagtcaag gcgacccagg cggttgacat tcatctcgcc
     3301 tgcccagact gttccgcgcg cgtccaccgc cagctgcatc agaccgacat tggtcgtcga
     3361 aaaccggtag agttgcttgc gggcggtttg cattacctcg gtgacaaggg gcgccgggac
     3421 agcatgtaca cgtacctggc gaacggaagg catactgaac ccagctgttg ccgcagcgct
     3481 aatgcgcccg ctcgtctcct gcatcccaca cccgctcgca agtatgagca gcacacagaa
     3541 agcgaaccag acaggcacaa gcaaggcaga gagtcgcttg tatcttcgaa ctcgctcatg
     3601 aagaattacg atcccgtgaa tagcgcttcg atccagtgtc cgcagtttct tctctgttcg
     3661 atccataatc cccagccttt tatcactgga ctgtcagggt gccgagcatg tatcctctgg
     3721 tcaccatggg accttgccag ccaactgcgc ccgttccgca gggatcaaaa catcggaagt
     3781 agtagaggcc cgcctcgcca gtgcggaagg taaagctgac ctcaacgtag gtcttgcctg
     3841 cagccgcatc cccaggcaca ggtacgttaa ttcccatctg ctgaatagtg aaggtatgag
     3901 ccaccttctc cggcgccaga gatgtgtagg cgcgcccatt cacgtaagcg acgccgccca
     3961 cgactccctg gactgcgctg aaaggagacc cggcgagcag aggggtatcc cccaggtcgt
     4021 agttacgaat agtgatggtc accaggctgt gggccggaac tacgagggcg ttgggacgat
     4081 aggcaggcca atcattctgc atgccgggct ggttgaggac gatcttcaga ttcgcctgta
     4141 catggcgctc gatctgggcg gcatgcgcaa tcgtcggcgc atccccactg cctgacgcgg
     4201 cttcaaagat gaagtaaata cctgatgaaa tggcgacgca gagcaacatg ccaaagataa
     4261 gacctaccga catgattgcc agtgatgtaa gaggacgctt tccccgctga agtgggattg
     4321 tattttgtgg ctgtgccatg ttctttcctc cgtagctata gggggaagca atccccctgg
     4381 gatttgtaaa gagcgtactt cacaaatctt aaatctaaat gagaaaaaca cagggaaaga
     4441 tcacaacgtt gtcacaagaa catagggaac aaatgcacat accggtgcat gtttctagac
     4501 acaccatgca caaatatcac cagaatttca tcggcatcgt caatcagacc ggcatatcaa
     4561 ggtgctaccc tgtaaatggg gaattgccaa caacgccaac gacgcctaca tgcatagcag
     4621 cgtggaaagg atagtgcctt gaacgatata ttcataccgc tcgtggacct gcaagctcaa
     4681 tttcaaagca tcaagcatga agtcatgccc gctatagaac acgtcctgga
//
LOCUS       DASZAK010000327         3869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_576405, whole genome shotgun sequence.
ACCESSION   DASZAK010000327 DASZAK010000000
VERSION     DASZAK010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576405"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1097
                     /locus_tag="VGT44_05795"
     CDS             126..1097
                     /locus_tag="VGT44_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV2580347.1"
                     /translation="MQSIQDLASAVQRVVNNVEKVIVGKAEPVAFSLIAVICRGHVLI
                     EDVPGVGKTVLTKAIARSIGCTFKRIQFTPDLLPSDVTGVSIYNQKTGNFEFRPGPVM
                     AQIVLADEINRATPKTQSALLEAMEESQLSVDGITHHLPEPFMVMATQNPIEYEGTFP
                     LPEAQLDRFMMNIKLGYPKVADEMNILDTHQYHHPLDDLAQIMTAEELVQIQQQVRAI
                     HVDPTIREYIVSIANATRSHANIYLGASPRGSLALYRGSQALAAMRGRGYVIPDDVKL
                     LVKPTLAHRIIVTPAARVRSVTSTAVLDEIMQTVPVPGAWVGGGKGR"
     gene            1101..2312
                     /locus_tag="VGT44_05800"
     CDS             1101..2312
                     /locus_tag="VGT44_05800"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV2580348.1"
                     /translation="MRPWQAIVLVIVLAFFAISSGWHPLYILMYVLLCLLLLAWLWAR
                     YSLRHMVFRRTSAVGRVQVGETFEERLMLDNESTLPKLWVQIADGSTLPGHRAGYVAS
                     MGGRKRATWRARTVCKQRGRFQLGPVIATSGDPFGMFRRRISLTESRELLVLPRVLPI
                     ASFVLFSGGLPGRGRSSQRALQTTTNANTIREYMSGDALNRIHWRSSAHYSKLMVKEF
                     DLDPAMDAWIILDLHDSVQAGEGEESTEEYGVTIAATIANFLLRQDLSLGMIVNAEHR
                     EFLSLDRGDRQIERTLEMLAVVRAGAGPDLKEVLALESFHFGRNTMAVIITPSASRDW
                     HDGVRHLQRRGVQVSVVGLDAASFDNRPADEDTLALLEGANIPVLRVECGTSLTEALE
                     KDPVARFAVHR"
     gene            2331..>3869
                     /locus_tag="VGT44_05805"
     CDS             2331..>3869
                     /locus_tag="VGT44_05805"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEV2580349.1"
                     /translation="MQETSVERLSKIIGERRQMPGGLNDNRPSPQRNVFAHVHLGLAE
                     GWFSLFLLFLVVYSGIWSVQAADWVENLGILPITTAFGLLLGVAAAKQRRFPRLVVHP
                     LAILFGILLAFWQTAGADKNGSVVALIDGMRQWVTLAINGGTSSDDSIFLFLITVLGF
                     LLAYTSAWLLFRTRSPWLMIVANAIVLLINLNNIDPGYIVFLVLFLVASLLLLLRFNL
                     YESSVRWKRLGLRTSEDLGWEFMQAGAMISIGILILTWLLPWGYINDAASQVWQIDNN
                     PLVFIQDQWNRLVSVSGGVNPQNHGNFTDMLVLGGNPNLTNDIVFKVKSDDGSQYLET
                     LSYDTYDGRGWSNGPSQAVPLKSGDAFTDSSIDLRAINQQIQVVNPPGEQSPYLLGAS
                     QLASTNQPVEVLLRKSDDTVVTWLRTNGKLAAGDTYTVTSYVSAADITSLEAIPLPKN
                     APSYTPNPDLPDLTPPPGYYDPTIVNTYTELPKNLDPHILATALQVTAGAKTMYDMAA
                     DLESY"
BASE COUNT          765 a         1126 c         1117 g          861 t
ORIGIN      
        1 agccgattac cagggtgccg tatttcatct tttcgcgcgc gttcgtgcgt ggtacactat
       61 ggacagcgtt tgtcatttgt tcaccggtgc tgagataaca caatcatcca atgtagggag
      121 atagtatgca gtctattcaa gacctcgcca gcgcagtaca gcgagttgtg aataatgtcg
      181 aaaaagttat tgtggggaag gccgaaccgg tcgcttttag cctgattgct gtgatttgtc
      241 gggggcatgt cttgatcgag gacgtgccgg gcgtaggcaa aaccgtgctg accaaagcca
      301 ttgctcgctc gattggatgc acgttcaagc gtatccagtt tacccccgat ctgttgccca
      361 gcgatgtaac gggcgtttcg atatataatc agaagaccgg caattttgag ttccgcccag
      421 gcccggtcat ggctcagatc gtgctggcgg acgagatcaa ccgcgccacg ccgaaaacgc
      481 agtcagcgct gctggaggcg atggaggaga gccagctctc agttgatggt attacgcatc
      541 acctgccgga gccattcatg gtgatggcca cgcagaaccc catcgaatac gaaggcacct
      601 tcccactgcc ggaagcccag cttgaccgct tcatgatgaa tattaagctc ggctatccaa
      661 aagtggctga cgaaatgaat attctggaca cgcaccagta ccatcacccg ctagatgacc
      721 tggcgcagat tatgaccgcc gaggaactcg tacagattca gcaacaggtg cgcgctattc
      781 acgtcgaccc gacgatccgt gaatacatcg tctcgattgc caacgccacg cgctcccatg
      841 ccaatattta cctgggagcc agcccgcgcg gctcgctcgc gctctatcgc ggctcgcagg
      901 cgctggcggc tatgcgtggg cgcggttacg tcatccccga tgatgtgaag ctgctggtga
      961 agccgacgct ggcgcaccgc atcatcgtca cgccggctgc gcgcgtgcgt tctgtcacct
     1021 caaccgccgt gctggatgag attatgcaga cggttccggt tcctggcgcg tgggtcggcg
     1081 gcggaaaggg gcgttgatcg atgcgaccct ggcaggcgat tgtcctggtg attgtgctgg
     1141 cgttcttcgc catcagcagt ggctggcacc cgctttacat actgatgtac gtgttgctct
     1201 gcctgctgct cctggcctgg ctttgggcgc gttacagcct gcgccacatg gtctttcgcc
     1261 ggacctctgc cgtggggcgg gtgcaggttg gcgaaacatt cgaggaacgc ctgatgctcg
     1321 ataacgaaag tacgctcccc aagctgtggg tgcagatcgc cgatggttcg accctgccag
     1381 gccaccgcgc cggatatgtc gccagcatgg gcggacgcaa acgtgccacc tggcgcgcgc
     1441 gcaccgtttg caagcagcgc ggacgcttcc agctcggccc ggtaatcgcc acgagcggcg
     1501 acccgttcgg catgttccgc aggcgtatct ccctgaccga gtcgcgcgaa ctgctcgtgc
     1561 tgccgcgggt gctgcccatc gccagctttg tgctgttctc cggcggcttg ccgggacgcg
     1621 gacgttcctc gcaacgcgcc ctgcaaacca ccacgaacgc gaacaccatt cgcgaatata
     1681 tgtccggtga cgcgctcaat cgaatccact ggcgttcgag tgcgcactat agtaagctta
     1741 tggtgaaaga attcgatctc gatcccgcca tggatgcctg gatcattctc gatttgcatg
     1801 actcggtgca ggccggcgag ggcgaggagt caacggagga atacggcgtc accattgccg
     1861 caaccatcgc caacttcttg ctgcgccagg atctgtcgct cggtatgatc gtgaacgccg
     1921 agcatcgcga atttctctcg ctggaccgcg gcgatcggca gatcgagcga acgttggaaa
     1981 tgctggcagt ggttcgcgcc ggcgcagggc cggatttgaa agaagtctta gctctggagt
     2041 cgttccattt cgggcgtaac acgatggcgg tcatcatcac gccgtctgct tcgcgcgact
     2101 ggcatgatgg tgtgcgtcac ttacaaaggc ggggcgttca agtctccgtc gtcgggctgg
     2161 acgccgcatc gtttgacaat cggcccgcgg atgaagatac cctggcgctg ttagaaggcg
     2221 ctaatattcc tgtgctgcgt gttgaatgcg ggacatccct taccgaggcg cttgaaaaag
     2281 acccggtagc tcgtttcgcg gtacacaggt agccctgcgg aggcacatcg atgcaggaaa
     2341 cgtctgttga aagactttca aaaattatcg gcgaacgacg ccagatgccc ggcggcctaa
     2401 acgataaccg gccttcgccg caacggaacg tgttcgcgca cgtgcatctc ggcctggcgg
     2461 agggctggtt ttcgttgttc ctgctcttcc tggtcgtcta cagcggcatc tggagtgtgc
     2521 aggcggcgga ctgggtcgaa aatctcggca tcctccccat tacaaccgct tttggactgc
     2581 tgctcggtgt ggccgcggcg aaacagcgac gctttcctcg cctggtcgtc cacccgctgg
     2641 cgatcctctt cggcatcctg ctggccttct ggcagacggc gggcgcggac aaaaatggca
     2701 gcgtagtcgc gctgatcgat gggatgcgcc aatgggtgac gctggccatc aatgggggga
     2761 caagcagcga cgactcgatc ttcctctttc tcatcactgt cctgggcttt ttgctggcct
     2821 ataccagcgc ctggctgctc ttccgcacac gcagcccctg gctgatgatc gtcgccaatg
     2881 cgattgtgtt gctgatcaat ctgaacaata tcgatcctgg ctatattgtg tttctcgtcc
     2941 tgttcctggt tgcttcgctt ttgttgctgc tgcgtttcaa tctgtacgag tcttcggtgc
     3001 gctggaagcg cctggggctg cgtacatcgg aagatctggg atgggagttc atgcaggccg
     3061 gagcgatgat ctcgataggc atcctgatct taacctggct gctgccctgg ggctacatca
     3121 atgacgccgc ttcgcaggtc tggcagattg acaacaaccc cctggtgttt atccaggacc
     3181 aatggaatcg cctggtttct gtttcgggcg gcgttaaccc acagaatcat ggcaacttca
     3241 ccgatatgct ggtgctgggt ggcaatccca atctgaccaa cgatatcgtc ttcaaagtga
     3301 aatcggatga tggcagccag tacctcgaaa cgctgagtta tgacacctac gatggcagag
     3361 gctggtcgaa tggtccttcg caagcggtcc ctctcaaatc gggcgatgcc tttaccgatt
     3421 cctcgattga cttgcgtgcc attaaccagc agattcaggt ggttaatccg cccggcgagc
     3481 agagtcctta cctgctgggc gcttcacagc ttgcctcaac caatcaaccc gtggaggttt
     3541 tgctgcgcaa atctgatgac accgtggtga cctggctgcg cacgaatggg aagctggcag
     3601 caggcgatac gtataccgtc acgtcctatg tctcggcagc agacatcaca tctctagaag
     3661 cgattcctct gcccaagaac gcgccttcat acacgccaaa tcctgaccta cccgatctca
     3721 cacccccgcc cgggtattat gacccgacca tcgtaaatac ctatacggag ctgcccaaaa
     3781 atctcgatcc gcacattctt gcaacagcgt tacaggtgac ggcgggcgcg aagacgatgt
     3841 atgacatggc cgcagacctc gaaagctac
//
LOCUS       DASZAK010000328         4088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_612531, whole genome shotgun sequence.
ACCESSION   DASZAK010000328 DASZAK010000000
VERSION     DASZAK010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612531"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VGT44_05810"
     CDS             <1..849
                     /locus_tag="VGT44_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580350.1"
                     /translation="GNGKHTMKTTTISTTSYRFAQHTRRINMLITIAAPVLVIAVALI
                     LLAALNAFGSGSSGLGVLGYLLAVPAFVLLIPMLLLTREYRRSGIWLDEEGIRARFPG
                     EKPQEMAWSEARFVVNEGEEYLQVSKGKEGLGHVVGDTRYLRLHLEGLLAEERQLALS
                     VLAERLPVREPTLFTLITLMNEKGETQARGRLYLFEHELLCTENRGEKRVFFYAPLKD
                     LSSVKPHNPYYIGRLQCDAFSLRYRGQDYVIMLGYEMTVSGNLGKSSRWSMTGNAEEW
                     VEALKQ"
     gene            1151..1357
                     /gene="rpmE"
                     /locus_tag="VGT44_05815"
     CDS             1151..1357
                     /gene="rpmE"
                     /locus_tag="VGT44_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEV2580351.1"
                     /translation="MKKGIHPKYVEATVSCACGNSFQTVSVKPLLKVDVCSKCHPFFT
                     GQQRILDTAGRVERFRRRFNLPEE"
     gene            1477..2979
                     /locus_tag="VGT44_05820"
     CDS             1477..2979
                     /locus_tag="VGT44_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2580352.1"
                     /translation="MNGQERVILFLIADTGAGHRSAANAIRNAISIIVQREQEQWLAL
                     QQESLAQETPHPSAAPDIPVTTTPIAGSLASSSSTPEPPTYRIEIVDAFEEYGRFPLR
                     EAMKLYGPAIRYNPRLYGQVFHLSNQAQTVSAVQSVATPLILNGLLRLIRGVQPDVIV
                     SIHPLLNHVTLRVLQDLEVRIPFVTVVTDLVSLHYSWFAPGADAYIVPTEHAKKLYLA
                     RGLDARRVHMLGMPIDPKFTLPTESKEELQRKLGLTPGLPVVLLVGGGEGSGGLRSSA
                     QAISQARLPVQLLIVTGRNKRLYMHLQRIRSSLLVPAKIYGFVQNMPEMMRASDVIIT
                     KAGPGTICEALSCGLPVILSAFVPGQEEGNVKFVTENDVGALAYDSLELVNMLRRLIK
                     PGSQVMRQQLENAKRIARPNASFDIAGAILGFLPAAGEPGVWEQPSGNHAGRRATPVR
                     RLQRVVSARLLRARRQAQARSVKTVITIKTFRHRLQRLKIIDRTIGRQRE"
     gene            3033..4049
                     /locus_tag="VGT44_05825"
     CDS             3033..4049
                     /locus_tag="VGT44_05825"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEV2580353.1"
                     /translation="MATFYYGGQAVMEGVMMRGRVSAAMAVRKPDNSIYLYEEALNPR
                     LYQSTLFRLPFLRGMLLLWQMLVLGTRLMTLSANIATGALDPDDAAPVEEMPLGAPTE
                     AGQEMPGRLDAPPQIGGIALIFTLLVSLGFAVAVFFLGPLLITGLLHNQIGVGLLSLL
                     IEGVIRLALLIGYLYVIGRIPDIQRVFGYHGAEHKAINAMEHGDPLDIPHVRRASRVH
                     TRCGTGFLLLVVVVSIFVFALVGSPSLFIKVISRIVLVPVVAGIAYELMRLGAANYCY
                     RVVRWLLAPGLALQGLTTREPDDEMIECAIAALRRVMRRDDQGFDAGPGEPDAALPPL
                     VVSN"
BASE COUNT          820 a         1115 c         1215 g          938 t
ORIGIN      
        1 ggcaacggga agcatactat gaaaaccaca acaatatcga caacatccta tcgtttcgcg
       61 cagcacacgc gccgtatcaa catgctcatt accatcgcgg ccccggtgct ggtcatcgcg
      121 gtcgcgctga tactgctggc ggcactgaac gctttcggca gcgggtcaag cggcctgggc
      181 gtcctcggct atctcctggc tgttcccgct tttgtgctac tgattcctat gctgctgctg
      241 acaagagaat acaggcgttc cggcatctgg cttgacgagg agggcattcg cgcgcgtttt
      301 cctggagaga agccacagga gatggcctgg tcggaggcgc gtttcgtcgt caacgagggc
      361 gaagaatacc tacaggtgag caaaggcaaa gaggggttgg gtcacgttgt cggcgatacg
      421 cgctatctgc gcctgcacct ggaaggcttg ctcgccgagg agcgccagct agctctgtcg
      481 gttctggctg aacgtctccc ggtgcgcgaa ccaacgctct ttacgctgat aacgctgatg
      541 aatgagaaag gcgaaacgca ggcgcgcggc aggctgtatc tctttgagca tgagttactc
      601 tgcaccgaaa atcgcggcga gaagcgtgtc ttcttctacg caccgctcaa agacctgtct
      661 tctgtgaagc cgcataaccc ctactacatc ggcaggctgc aatgcgatgc cttctccctg
      721 cgctatcgcg ggcaagatta cgtgattatg ctggggtatg agatgaccgt gtcagggaat
      781 ctggggaaaa gctcacggtg gtcgatgacg gggaacgcgg aggagtgggt ggaggcgcta
      841 aagcaatagt acgaaagggc gaccgcaagg gtacgccctt cctcttgagg gcataaactc
      901 ctccctaaat ttgttatctt atgatagaat atggtcaata agccgagttg taccgtattg
      961 cctggggtgg cgcgtgggag gaacaaggaa tctcctgaac tcctaacctc ttgacggcct
     1021 gcggtcgtcc gtgtacaatc atgctcacag ccacactgtt gaatactacc ttacacatca
     1081 ctgctgacac gcagtgtagc gcgctagcgg cgttgcagtg gtgtatagcg aggatgaatt
     1141 gggagttgtc atgaaaaagg gaatacatcc gaaatatgtt gaggccacag tcagttgcgc
     1201 ctgtgggaat tctttccaga ccgtcagcgt aaagccactt ctgaaggtgg atgtttgctc
     1261 aaaatgccat ccgttcttca ccggacagca gcgcattctt gacacggcag gccgcgtcga
     1321 gcgtttccgc cggcgcttca acctgccgga agagtagtct ggttagcgta ggaaatcacg
     1381 gggataaagg cgtgccacat tgtgccttta tcccctgttc gcgctttgcg tgttcggtat
     1441 ttcatcgacc gtcaaattaa aaagaggaac gacccattga acgggcagga gagagtaatc
     1501 cttttcctga ttgctgatac cggggcaggg catcgcagcg ccgctaacgc cattcgcaat
     1561 gcgatcagca ttattgttca acgtgagcaa gaacaatggc tcgcccttca gcaagaaagc
     1621 ctcgcacaag agacgccgca cccctcagca gcgcctgata ttcctgtgac tacaacgcct
     1681 atcgccggtt ctttggcatc atcgtcgtca actcctgagc cgccaacgta tcgtatcgaa
     1741 attgtcgatg ccttcgagga gtatggccgg tttccgctgc gcgaggcgat gaaattgtat
     1801 ggcccggcca ttcgctataa tccccggtta tatgggcagg ttttccacct gagtaaccag
     1861 gcgcagaccg tttcggcggt gcagtcggtc gcgactcctc tgatcctgaa tggtttgctg
     1921 cgcctcattc gcggtgtgca acccgatgtg attgtttcga ttcatcccct actcaaccat
     1981 gtgacgctgc gcgtcttgca agacctcgaa gtgcgcatcc cttttgtcac ggtggtgact
     2041 gacctggtca gtttgcacta ttcctggttc gcgccgggcg ctgacgccta tattgtgccg
     2101 accgaacacg cgaagaagct ctacctggcg cgcgggctgg atgctcgccg ggtgcatatg
     2161 ctggggatgc cgattgatcc caagtttacg ctgccaaccg aaagcaaaga agagttacag
     2221 cgcaagcttg gactcacccc tggactgccg gtggtgttat tagttggcgg gggcgaaggt
     2281 tccggcgggc tgcgttcgtc ggcccaggcc atctcgcaag cgcgattgcc ggtgcaactg
     2341 ctgattgtga caggcaggaa caagcgcctg tatatgcatt tgcagcgcat tcgctccagt
     2401 ctgctcgttc ccgcgaaaat ctacggtttt gtgcaaaaca tgccggaaat gatgcgcgcc
     2461 tcggatgtga tcatcacaaa ggccggtccc ggcacgatct gcgaggcgtt gtcctgcggt
     2521 ttgccggtga ttctcagcgc ctttgtccct gggcaggaag aaggcaatgt caagtttgtg
     2581 acggaaaacg acgttggcgc gctggcgtat gattcgctgg aactcgtcaa tatgctgcgg
     2641 cggctcatca aaccaggctc gcaagtcatg cgccagcagc tcgaaaacgc gaagcgtatt
     2701 gctcgcccca atgcatcgtt tgatattgca ggcgctattc tcggttttct ccctgctgcc
     2761 ggcgaacccg gcgtatggga gcaaccgagc gggaatcatg cggggcgacg cgccacacct
     2821 gtgcgcaggc tgcaaagagt cgtctcggcg cgtctcctgc gcgcccggcg tcaggcgcag
     2881 gcacgctcag ttaaaacagt aattacgatt aagacgttcc gccaccgttt gcagcgattg
     2941 aaaatcatcg atcgcaccat tggcaggcaa cgcgagtgaa atgcaaattg aatgtgtaga
     3001 gcgcaattga ttgccggata aggacgagag atatggcaac attttattat ggcggccagg
     3061 cagtgatgga gggggtgatg atgcgcgggc gcgtgtcggc tgcgatggcc gtgcgcaagc
     3121 cagataacag catctacctc tatgaagagg cgttgaaccc gcgcctgtat caaagtactc
     3181 tgtttcgctt gccattcttg cgcggcatgt tgctgctctg gcagatgctt gtactcggta
     3241 cgcgcctgat gacgctctcc gccaacatcg ccaccggcgc actcgatcct gatgacgcgg
     3301 ctccggtcga ggagatgcca ctcggtgcgc ccacggaggc gggacaggag atgccgggtc
     3361 ggctcgatgc gccaccgcag attggcggga ttgcgctgat tttcacgctg ctggtctcgc
     3421 tgggatttgc cgtggctgtc ttcttcctgg ggccgctgct tattaccggc ctgctgcata
     3481 atcaaatagg cgttgggctg ttgagcttgc tgatcgaggg cgtcattcgc ctggccttgc
     3541 tgattggcta tctgtacgtc ataggccgca ttcccgatat ccagcgtgtc tttggctatc
     3601 acggtgcgga gcataaggcc atcaacgcca tggagcatgg cgatccgctc gatatcccgc
     3661 atgtgcgccg cgcctcgcgt gtgcatacgc gctgtggaac gggatttttg ctgctcgtgg
     3721 tggtggtctc catttttgtc tttgcgctgg ttggctcgcc gtctttgttt atcaaggtca
     3781 tctcgcgcat cgtcctggtg ccggttgtgg cgggtatcgc ctacgaattg atgcgcctgg
     3841 gggcggcgaa ctattgttat cgcgttgtgc gctggctgct cgcgccggga ctggctttgc
     3901 agggcctgac gacgcgcgag ccggacgatg aaatgattga atgcgccatt gctgcgctgc
     3961 ggcgtgtcat gcgccgcgac gatcaagggt tcgacgcagg tccaggcgag cctgatgccg
     4021 cgctgccccc actggttgtc tcaaactaaa aaggatagat tcatgtcgtc tgacccgatc
     4081 atcacgcc
//
LOCUS       DASZAK010000329         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_399014, whole genome shotgun sequence.
ACCESSION   DASZAK010000329 DASZAK010000000
VERSION     DASZAK010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399014"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            530..2053
                     /gene="murJ"
                     /locus_tag="VGT44_05830"
     CDS             530..2053
                     /gene="murJ"
                     /locus_tag="VGT44_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEV2580354.1"
                     /translation="MSVSTIEPEADIVIATPEEVSMEDEVEDEVTSQQPHIGFGVFRF
                     RFNLANFRLGRDISDRRFSIAEAALLLMAAYLASRGLGVVRQSIFNALFGTSDAANAY
                     YAAFRLPDFLFNLIAGGALTHAFVPVFVTFDKEKGRQAAWRLTSLIFNIMLVSLTAIV
                     LVGEILAPVFVSHILVPGYSAQVQDLTTSLTRIMLIQPLILGLGTIATSVLSSKRQFL
                     LPALSIAVYNFGLIAGLVCTLIYPPLGIYGPTYGVLLAALCQVVVQIPGLVRQGIRYS
                     FVWNLRDSGLRQVLNLLGPNALIVMLGSTAFIIDTAFASYLPDLASLSAQHNAYMLFY
                     VPVALLSQAVSQPVLPQLSLLAARGNFLRMQRLTARVTGASLLLGIPATLGLCILGKP
                     VIHLIFQHGAFDSHSTTLTALALIGYAVGLPAIIASEMFVRAFFAVKDARVPLFTNIF
                     NLSVHIALILTLLKVYTGQNAILAIPLAASGSATAECILLGTLLYFRLRRKIRKEMS"
     gene            complement(2063..>2678)
                     /locus_tag="VGT44_05835"
     CDS             complement(2063..>2678)
                     /locus_tag="VGT44_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2580355.1"
                     /translation="TLHTLGYLATQRSDLAEASACYQEGLSIALDIGNKQHIGLHLIG
                     LAGVALAQEQPRRATRLFGAAGSVLDIEAELNADERAAYERGIEQARAIVGEETFASL
                     HVEGRDMSPEAALAASEQAQPARVATHLPTHPSKAAYPDGLTQREVEILRLLATGHTV
                     AQMAGHLVISARTVSTHITTIYRKIGVNSRSDATRYAIAHKLVK"
BASE COUNT          494 a          795 c          748 g          641 t
ORIGIN      
        1 taatagttgt gctttgttct tagcacactg tacctgagtg aacgcaatat gtcagcttgc
       61 ttcgcacagg gtagggtaaa taggctattg gcgctagagc gcatcttaac cccccaccat
      121 ggctgttgca gacaccaggt atgcgtctca caagtgaaat aaacaggagg attgatgctt
      181 ttggcgcaaa cgctcgattt tgattttccc aacctggttc agccctgtca ccctgagcga
      241 agggtctcgg ctgttggacc gcgagatcct gagcgaagcg aaggataaca gagccaactt
      301 tggtctgaaa attcattatc tatgactcct tgtgatgttt gggcttttca atcgttgtac
      361 acggtacagg ggggaagtag acaatagtac ttgtggtgcg caaattccat actattgcat
      421 tgttcactgg tataatacac agcgcagaga ctggaaatac acgcgcaaga gggaagagga
      481 ggagcaggct atttcacagg aacctactca atcaagcaaa ctaccggtca tgtcagtctc
      541 aaccattgag cctgaggctg atattgttat tgccacgcca gaagaagttt cgatggaaga
      601 tgaagttgaa gacgaggtga cttcccagca gccccatatc gggtttggcg tctttcgttt
      661 tcgcttcaat ctagccaatt ttcgcctggg ccgcgatatt tcagatcgtc gtttcagtat
      721 cgcggaggcg gctctgctgc tcatggcggc ctatctagcc tcgcgtgggt tgggcgttgt
      781 gcgccagagt atcttcaacg cgctttttgg cacgagcgat gccgccaatg cctactacgc
      841 ggcttttcgc ctgcccgact ttcttttcaa cctgattgcc ggcggcgcgc tgacacatgc
      901 gttcgtcccg gtcttcgtca ccttcgacaa ggaaaagggt cgccaggccg catggcgcct
      961 aaccagcctg atcttcaata tcatgctcgt cagcctgacg gcgatcgtgc tggtaggcga
     1021 aatcctggcg ccggtcttcg tcagccatat cctcgtgcct ggctatagcg cacaggtgca
     1081 ggatctaacg acttccctga cccgcatcat gctgattcag cccctaattc tgggacttgg
     1141 cacgattgcc acctcggtct tgagcagcaa gcgccagttt ttactcccgg ccctctcgat
     1201 tgccgtgtac aatttcggct tgatcgccgg cctggtctgc acgctgatct acccgccact
     1261 gggcatctat ggtccaacct atggcgtact gctggccgcg ctatgccagg ttgtagtgca
     1321 gattcccgga ctggtgagac aggggatacg ctattcgttc gtatggaatc tgcgagactc
     1381 cgggctgcga caggtgctca acttgcttgg tccgaacgcg ctgatcgtca tgctaggctc
     1441 tacggccttc atcatcgata ccgccttcgc ttcttacctg cccgacctgg ccagcctgtc
     1501 cgcccagcac aacgcctaca tgctcttcta cgtgccggtc gcactgctct cccaggcggt
     1561 atcgcagccg gtgttgccgc aactatcgct gctggcggcg cgagggaact tcctgcgtat
     1621 gcagcggctc acggcaagag tgacgggcgc ttcactgctg ctgggcatcc ctgccacgct
     1681 tggcctgtgc atcctgggca agccggtcat tcacctcatc tttcaacacg gcgcgttcga
     1741 tagccattcg acgacgctga ccgcgcttgc tctgatcggc tatgcggtag gcttacccgc
     1801 cattatcgcc tcggagatgt tcgtgcgcgc cttcttcgcc gtgaaggatg cgcgcgtccc
     1861 gcttttcaca aacatcttca acctctccgt gcatatcgcg ctgatcctca ccctgctcaa
     1921 ggtctatacc ggccagaacg ccatactcgc catcccactt gccgccagtg gttcggccac
     1981 agccgagtgc atcctgctcg gcaccctgct ctatttccgc ctgcgccgga agatacgcaa
     2041 ggaaatgtcg taggggcgca tgtcacttca ccagtttatg cgcgatggcg tagcgggttg
     2101 cgtcgctgcg ggaattcacg cctattttgc ggtagatcgt cgtgatgtgc gtactgacgg
     2161 tgcgcgcgct gatgaccagg tgtccggcca tttgcgctac cgtatgcccg gttgccagca
     2221 ggcgcagtat ctcaacctcg cgctgcgtca gcccgtcggg ataggcggct ttggaaggat
     2281 gtgtgggtag gtgtgtggct acgcgggctg gctgcgcctg ctccgatgcc gcgagcgccg
     2341 cttctggcga catgtcgcgt ccctcgacat gcagcgaggc gaatgtctct tcacccacga
     2401 tggcgcgcgc ttgctcgatg ccgcgttcgt aggcggcgcg ttcatcggca ttcaactccg
     2461 cttcgatgtc gaggactgat ccggctgcac caaagaggcg cgtggctcgc cggggctgct
     2521 cttgcgccag ggccacaccc gccaacccga tcaggtgcag gccaatatgt tgcttgttgc
     2581 ctatatccag ggcgatgctc aagccctcct ggtaacaggc cgaggcttcc gccaggtcgc
     2641 tgcgctgtgt cgcgagatag cccagggtat gcagcgtc
//
LOCUS       DASZAK010000330         3576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_540693, whole genome shotgun sequence.
ACCESSION   DASZAK010000330 DASZAK010000000
VERSION     DASZAK010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540693"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGT44_05840"
     CDS             complement(<1..206)
                     /locus_tag="VGT44_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580356.1"
                     /translation="MAVSTRPQQQGAARRVWHTPTSKAALYWFLFAGIYCLIFYVLYA
                     VFFNAHIFTGPWFDRIFRAGVMSY"
     gene            complement(219..2165)
                     /locus_tag="VGT44_05845"
     CDS             complement(219..2165)
                     /locus_tag="VGT44_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV2580357.1"
                     /translation="MSVQSERQPLVVGIQHRTDLVPLTEDEVTFEIDMCIGCNRCMRA
                     CPVPLSSQVSIADLNLATISDEIAPHVAKFTDQCVMCGSCVPVCPVNNHRDLLMLSLK
                     QRLGISWDGNVDMSRVLDYLPVGWNVMLLLSRLREQPMFSDPQQLPDNYLLHFIADSK
                     IESVLPGTVVLHEGEYGRNMLLILEGRFTISSAGPNGADVPAAVLRRGEFMGERGMLT
                     GQQRNATARAQTAAVVLDVPEQVMQRMMEIVPAVRNFFERLNNARSIESVLKRMAVFQ
                     GISGQDMSWIASKIQFKNYDRDEPLFTESDAGQPARESLHIIIEGFVKVARRTTARTG
                     RNKTDERIIAYRQGGDYFAGGLDLLGDGRAVTVTAINRVRVAEIPRQDMLTLLGRYPE
                     VNQRFLTHLQEYRTAAEAAQTFIGPMDFLRSASSQSFADAEARAGLGALVGGGVVEGT
                     EVLVIDLDKCVHCDKCEDACERRHGHSRMNRKGMVVGNISIATACRQCQDPVCMLCSR
                     AGIARMPSGEVYITESCIGCGICAERCPYDNISIVSLADEVEERSALQRFASFFTKGT
                     GTERGKKMLPMANNIASPGPLNIQQPLDAFGEMRKKLAIKCDLCAGYNNQACVQACPT
                     GAAFRTQPVQFFGSTEEILQRRVQ"
     gene            complement(2178..>3576)
                     /locus_tag="VGT44_05850"
     CDS             complement(2178..>3576)
                     /locus_tag="VGT44_05850"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2580358.1"
                     /translation="SVGISAIPKDGKGYQEIIADPQPRTYQKVILHNGVPVGMLALGD
                     RRSVLTFKRAIDAGVDLSPVASRLFAPDFKLSAWMDAQGVPRPILGVSREGAAAIQKA
                     AYADVGNRSAILKAQELTEAVLVPLVSQAPPDVQSALKETYLSQTKVTTIGRQEGSAI
                     AINHSSISRRHAEIGYAHGQYLLRDLKSTNGTFVNDQRLDPEGICQLKQDDKVRFGKV
                     SFALQMRHVDPSSSVLLRRPVVGAPPAANQSPAQAVAWELITYRNEDQELASGDFNDE
                     TVQIKHDGASPSSVQAPLGQPTLSADGSLQLPGATRPIPADVVARMMHAPTLVVVMRG
                     EPEIVLFKQGQRYILGRDKRSRILLADMSVSRKHAEVFPGLDGYYIRDLASSNGVMVN
                     QTRIDNPYRLSNGDRIMIGNVLLYFIDLSDHKMPLAPQVAKSPADVKVAKTCPGCGSE
                     NSSVARFCANCGASL"
BASE COUNT          805 a          983 c         1017 g          771 t
ORIGIN      
        1 atataggaca tgacgcctgc gcgaaatatg cggtcaaacc agggtccggt gaagatatgg
       61 gcgttgaaga acaccgcata taagacgtag aagatcaggc agtaaatacc ggcgaatagg
      121 aaccagtaca acgccgcctt cgaggtaggt gtgtgccaca cgcgccgcgc ggcgccttgc
      181 tgttgtggcc tggtgctgac agccattgtg tactcctcct attgcactcg ccgttgtaga
      241 atttcctcgg tcgagccgaa aaattggaca ggttgtgtgc ggaatgccgc gcctgtcgga
      301 caggcctgta cgcaagcctg gttgttgtag cccgcgcaga gatcgcactt gatagccagc
      361 ttcttgcgca tttcgccaaa ggcatcgagc ggttgttgaa tattgagcgg accgggtgag
      421 gcgatgttgt tcgccatcgg gagcatcttt ttcccgcgct ctgtacccgt gcctttggtg
      481 aagaagctgg caaaacgctg taaggcggag cgttcttcaa cctcatcggc aagcgagaca
      541 atcgaaatgt tgtcgtaggg gcagcgttcg gcacagatgc cacaaccaat acagctttcc
      601 gtgatgtaga cctcgccgct tggcatacgg gcaataccgg cgcgactaca tagcatacag
      661 acggggtcct ggcactggcg gcaggcggtg gcaatgctga tgttgcccac gaccatacct
      721 ttgcggttca tgcgcgagtg tccgtggcgc cgctcgcagg catcttcgca cttatcacaa
      781 tggacgcact tgtccaggtc gatgaccagc acttcggtgc cttccacaac gccgccgccg
      841 actaacgcgc ccaacccggc gcgagcttca gcatcggcaa atgattggga ggaagcgctg
      901 cgtagaaaat ccatcgggcc aatgaaggtc tgagcagcct ctgccgccgt gcgatactcc
      961 tggaggtgcg ttaaaaatcg ctgattgact tcgggatagc gccccaggag ggtcagcata
     1021 tcctggcgtg gaatctcggc gacgcgcacg cgattgatgg cggtcacggt gacggcgcgg
     1081 ccatcgccca gcagatccag gccgcccgcg aagtagtctc ctccctgtcg gtaggcgatg
     1141 atacgctcat cggttttatt gcgcccggtt cgcgccgtgg tacggcgcgc cactttcaca
     1201 aaaccttcaa tgattatgtg caacgattcg cgcgccggtt ggcctgcatc gctttccgtg
     1261 aagagaggtt catcgcggtc atagtttttg aattgtatct tactggcaat ccagctcata
     1321 tcctggcccg atataccctg gaaaacggcc atacgcttca ggacggactc gattgagcga
     1381 gcgttgttca aacgttcaaa gaagttgcgc accgcgggta caatttccat catgcgctgc
     1441 atgacctgct ccggcacatc cagcaccacg gcagcggtct gagcgcgcgc agttgcattg
     1501 cgctgctgac ccgttaacat gccgcgctca cccataaact ctccccggcg cagcaccgct
     1561 gccggcacat ctgcgccatt gggtccggca gaagagatgg taaagcgccc ttccaggata
     1621 agcagcatgt tccgcccata ttcgccttca tgtaacacca ccgtgccagg cagcacgctt
     1681 tctatcttgg aatcggcaat gaagtgcagc aggtagttgt cgggcagctg ctgtggatca
     1741 ctaaacatag gctgctcgcg caggcggctt agcagcagca tgacgttcca gccgacaggc
     1801 aggtaatcca ggacgcgact catatccacg ttgccatccc aggatatgcc gaggcgctgc
     1861 ttgagcgaga gcatgagcag gtcgcgatgg ttattgacag gacagacagg aacgcatgag
     1921 ccgcacatga cacattgatc agtaaatttt gctacatgcg gcgcaatttc atcagagata
     1981 gtcgcaaggt tgaggtcggc gatgctgacc tgggaggata aaggcacggg gcaagcgcgc
     2041 atacagcgat tgcagccgat gcacatatct atttcaaaag ttacttcatc ctcggtgaga
     2101 ggtacaaggt cagtgcggtg ttggatgccc acgacaagcg gttgtctctc cgattgaacg
     2161 ctcatacaaa cactccttta cagcgacgcg ccacaatttg cacaaaaacg cgctacgctg
     2221 ctgttttcag aaccacaacc gggacaggtt ttggccacct ttacatcagc cggagacttt
     2281 gcgacctgtg gagcgagcgg catcttgtga tcgcttagat cgatgaagta aagcagcaca
     2341 ttcccaatca taattcgatc gccgttggag agccggtaag gattgtcgat gcgcgtctgg
     2401 ttgaccatga ccccattgct actggccagg tcacggatat agtagccatc caggcccgga
     2461 aagacttcag catgtttgcg cgatacggac atatccgcga gaagaatacg actgcgtttg
     2521 tcgcgcccca gtatgtagcg ttggccttgt ttgaacagaa caatctcagg ctctccacgc
     2581 atcacgacaa caagcgtcgg cgcatgcatc atgcgagcca cgacatcggc aggaataggg
     2641 cgtgttgcgc cgggcagttg cagcgagccg tcagcgctta gagtaggctg acccagaggg
     2701 gcctggacac tcgatggaga tgccccatca tgcttaatct gcactgtttc atcattgaaa
     2761 tcaccacttg ccaattcctg atcttcattc ctgtaggtga tcagttccca ggctacggct
     2821 tgcgccggag attggttagc cgcgggcggc gcgcctacta ccggtctgcg aagaagcacc
     2881 gaagatgaag gatcgacatg acgcatttgt agagcaaacg agaccttacc gaaacgcacc
     2941 ttatcgtcct gtttgagttg acaaatgcct tctggatcga gacgctggtc attcacaaat
     3001 gtgccattgg tgcttttcag atcgcgcaag aggtattgcc cgtgagcata accgatttcg
     3061 gcatggcggc gcgagatgga gctatggttg atagcaatcg cggaaccctc ctggcggcca
     3121 attgttgtta ctttcgtctg gcttagatag gtttctttga gagcggattg tacgtcggga
     3181 ggagcctgtg agacaagcgg aacaagcaca gcctccgtca gctcctgcgc cttgaggatg
     3241 gcagaacgat ttcccacatc cgcataggcg gctttttgaa tcgctgcggc gccttctctg
     3301 ctcacgccca gaatgggtct tggcacaccc tgagcatcca tccaggcgct gagtttgaaa
     3361 tcgggggcaa acaggcggga agcgacggga gaaaggtcta cgcctgcatc gatagcgcgc
     3421 ttgaaagtaa gtacgcttct gcggtcgcca agcgcaagca tacccacagg cacgccatta
     3481 tgaagaatga ccttctgata ggtacgcggc tgcggatcgg caataatctc ctgataccct
     3541 ttgccgtctt tagggatggc cgagatgcca acggag
//
LOCUS       DASZAK010000331         1782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_150444, whole genome shotgun sequence.
ACCESSION   DASZAK010000331 DASZAK010000000
VERSION     DASZAK010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150444"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VGT44_05855"
     CDS             complement(<1..762)
                     /locus_tag="VGT44_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2580359.1"
                     /translation="MRKVISKDGTPIAFDQSGVGPPVILVVGAFNDRATGAPLAAQLS
                     EHFTVFNYDRRGRGASGDSTPYAIEREIEDLAALLDEAGGSSSVFGYSSGAILILKAA
                     ASGLALSRLALYEPPFLLDDGFPQRAQEISVRLTELVASGRRGDAVELFQMEMVGIPA
                     EIVAQMRHAPFRPALENIAPTLIYEATIMGDISALAEQLPSIRIPTLVISGGISQAIM
                     RSAAQTLADALPNAEHRSLEGQTHDIVPAVLAPVLE"
     gene            complement(810..1514)
                     /locus_tag="VGT44_05860"
     CDS             complement(810..1514)
                     /locus_tag="VGT44_05860"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HEV2580360.1"
                     /translation="MYQELTRRFIDLLQLNQPPIGLAFVDTAPQGVRKTGRRVPSACT
                     FWRLAEQGVFYATAEDHQECPIGMLTMGFSMPEAAQQRAQELVQTMASVQYFSPAEVS
                     ALPIVQKPHTSVVYGRLDQFPVQADVMLCVLDTRQAMLVAEAMGNVNWLQGGQAAFGR
                     PTCAVIPRAMQTGATSLSFGCVGARTYIDLQPSELVLTVPNDAFATLVERLQIIIDAN
                     AALAPFHAQQKARFSE"
BASE COUNT          361 a          499 c          544 g          378 t
ORIGIN      
        1 ctccaacaca ggagccagga cagccggaac gatatcgtgc gtctgacctt ccagggagcg
       61 gtgttcggca ttaggaaggg catcggcgag tgtttgcgcg gcagagcgca ttatcgcctg
      121 gctgattccg ccgcttatca cgagcgtggg gattctgatg gagggcagtt gttcggccag
      181 cgctgaaatg tcacccatga tagtagcctc atagatgagc gtgggcgcaa tgttttcgag
      241 cgccggcctg aatggcgcgt gtcgcatttg ggcaacgatt tcagcgggaa tgcccaccat
      301 ctccatctgg aagagttcga ccgcatcgcc ccggcgcccc gacgctacca gttcggtgag
      361 tcgcaccgag atctcctgcg ctcgttgtgg aaacccatcg tctagtagaa acggcggctc
      421 gtacaacgcc agcctggaca gagcgaggcc gctcgctgcc gccttgagga taaggatagc
      481 gccagaggaa tatccgaaca cggacgacga tccgccagct tcgtcgagaa gagctgcgag
      541 atcctcgatc tcccgctcga tagcataggg tgtgctgtca ccactcgcgc ctcgaccccg
      601 gcgatcatag ttgaaaacag tgaaatgttc tgaaagctga gcagccagtg gggcgcctgt
      661 ggcacgatcg ttgaatgcgc caaccaccag gatgacgggc ggtcccacac ccgactgatc
      721 gaaggcaatc ggagtgccat cttttgagat gacttttctc ataaaatctc tccttaaacg
      781 cccttatata gtaattgcgg agtgcctgac tactcgctga agcgcgcctt ttgctgtgcg
      841 tggaaggggg cgagggcagc attggcatca atgatgattt gcaggcgctc gaccagggtg
      901 gcgaaggcgt cgttgggaac ggtcagcacc agctcggatg gctgcaggtc gatatatgtg
      961 cgcgcgccga cgcagccgaa gctcagcgag gtcgcgccag tttgcatggc gcgcgggatg
     1021 acggcgcagg tagggcgtcc gaaggcagcc tgtccgcctt gcagccagtt gacgttgccc
     1081 attgcctcgg cgaccagcat ggcctggcgc gtatcgagaa cgcagagcat gacatccgcc
     1141 tgcaccggga actggtccag gcgtccgtac acaacgctcg tatgtggctt ctgaacgatt
     1201 ggcaacgcgc tgacctcggc aggcgagaag tattgtacgc tcgccatcgt ctgcacaagc
     1261 tcctgggcgc gctgctgcgc agcttccggc atgctaaagc ccatcgtcag catcccgatg
     1321 ggacattcct ggtgatcttc cgccgtcgca taaaagacgc cctgttcggc cagacgccag
     1381 aacgtacacg cggatggcac acgcctgccg gtcttccgca caccttgcgg cgcggtatca
     1441 acaaaggcta agccgatggg aggctgattg agttgcaaga gatcgataaa acgccgggta
     1501 agttcctggt acaatcgggt tcctctttct tgtgatacgg gtagcggaga cgctacgggt
     1561 atagtgtatc acaagggaat ccggagggcg tacccttgcg gtcgccctcc ggattccctt
     1621 gtcaggcatc ctcatagttt tgtcacacgg tcttatattt tctcgctaaa acgttgatcc
     1681 aattgctttg tttctcgctg ggtgcgaagg tgtttttctg gaagcgtttg gccatgtcgc
     1741 gaatgaattc acgctcgttc gcggcgatgc gcgggttgtt ga
//
LOCUS       DASZAK010000332         1930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_79714, whole genome shotgun sequence.
ACCESSION   DASZAK010000332 DASZAK010000000
VERSION     DASZAK010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79714"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VGT44_05865"
     CDS             <1..234
                     /locus_tag="VGT44_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580361.1"
                     /translation="IVLNPDEDSKNGAATLGLILRRFKALTTRTIRTTTESQFEWQRN
                     YHERIIRNENHLYEVRQYIIDNPLKWAEDELFT"
     gene            249..905
                     /gene="rpe"
                     /locus_tag="VGT44_05870"
     CDS             249..905
                     /gene="rpe"
                     /locus_tag="VGT44_05870"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV2580362.1"
                     /translation="MINIVPSILSADFTRLGDQVREAEAAGTQRIQIDVMDGHFVPNI
                     TMGPMIVEAVRRCTQLTLEAHLMITNPERYIDDFARAGADVIIVHQEVCPHLHRVIQQ
                     IRHAGKSAAVALNPSTPVFLLHEVLSLIDMVLVMTVNPGFGGQDFIPECLPKIERLRQ
                     AITQRGLHCDIEVDGGIHEATAPVAVKAGANLLVAGSAVYNERESVAQAIERLRNAVG
                     "
     gene            913..1308
                     /locus_tag="VGT44_05875"
     CDS             913..1308
                     /locus_tag="VGT44_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEV2580363.1"
                     /translation="MKPITVTVVTKQKDQVLDITDTIETHLRGAPEESGVCVVFAAHT
                     TCALTTADLDPGTDLDLLNALRHTLPDLSYRHPHDPSHTPDHILSSLIGPSVAIPYHN
                     GRLLLGTWQRVILVELDGPRHRTVHVAWM"
     gene            1380..1880
                     /locus_tag="VGT44_05880"
     CDS             1380..1880
                     /locus_tag="VGT44_05880"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV2580364.1"
                     /translation="MANRQHLSMLKQGVVPWNEWRKKNQDVRPDLNEANLNGVNLSGV
                     DLSAANLSGADLVSADLRAAHLSGATLQETNLREADLRKADLSFANLTFADLSDAKLA
                     DARLWKADLTAAKLVAADLSGANLTMATLRETDLSGANLQGAELIYAILVETNLKQTN
                     LQGCFI"
BASE COUNT          456 a          579 c          510 g          385 t
ORIGIN      
        1 attgttttaa atcccgatga ggattccaag aatggtgccg caactttagg tctaatttta
       61 cgcagattca aagccctgac aactcgcacc atacgtacca ctactgaatc acaatttgaa
      121 tggcaacgca attatcacga acgtatcatt cgcaatgaaa atcacttata tgaagtccgt
      181 caatacatca tcgacaaccc tctaaaatgg gccgaagatg agctatttac gtagaaaggc
      241 tatctcgcat gatcaatatt gtgccatcaa tattatctgc cgatttcacc cggctcggcg
      301 accaggtacg cgaggcagag gcggcgggca cgcagcgcat ccagatcgac gtgatggacg
      361 gccatttcgt cccaaatatc acgatggggc cgatgattgt cgaagcggta cggcgttgta
      421 cccaactgac gcttgaggcg cacctgatga tcaccaaccc ggaacgctac attgacgact
      481 tcgcgcgagc cggggctgat gtcatcatcg ttcaccagga agtctgtccg cacctgcatc
      541 gcgtcatcca gcagattcgc cacgctggca agagcgccgc cgtagcgctc aacccctcga
      601 cgcccgtctt tctgctccac gaagtgctat cgctgatcga tatggtactc gtcatgaccg
      661 tcaaccccgg tttcggcggc caggatttca ttcccgaatg ccttccaaaa attgaacgcc
      721 tgcgccaggc catcacccag cgcggcctgc attgcgatat cgaggtagac ggtggcattc
      781 acgaggcgac cgcgccagtg gccgttaagg ctggcgccaa cctgcttgtg gccggctcgg
      841 cggtctataa cgagcgcgaa agcgtcgccc aggccatcga gcgactgaga aatgccgtag
      901 gatgatggct atatgaaacc gattaccgta accgtggtca cgaagcaaaa agaccaggtt
      961 ctcgatatta ccgatactat cgaaacccac ctgcgcggcg cgcccgaaga gagcggtgta
     1021 tgcgtcgtct tcgccgccca cacgacctgc gcgctcacca ccgccgatct cgaccccggc
     1081 accgacctcg acctgctcaa cgcgctgcgg cataccctcc cggacttgtc gtatcgccat
     1141 ccacacgatc cctcgcacac gcccgatcac atcctctcgt cgctgatcgg cccatccgtc
     1201 gccatcccct accacaatgg caggctgctg ctggggacgt ggcagcgcgt gatcctggtt
     1261 gagctggacg ggccgaggca ccggacggtg catgtggcgt ggatgtgagg agctatgctt
     1321 tcaaaaacag gcggctttgt gtgctatact gatgcagatt ataacgccgg gggaaccata
     1381 tggcaaacag gcaacacctc agtatgctca aacagggcgt cgtaccctgg aacgagtgga
     1441 gaaaaaagaa tcaggatgtt cggcctgatc tcaacgaggc caacctgaac ggtgtcaact
     1501 taagcggagt tgacctgagc gcggcaaacc tcagtggggc cgatcttgtt tcggctgacc
     1561 tgcgcgcggc gcacctatct ggcgcaaccc tacaggaaac aaacctgcgc gaggctgatc
     1621 ttcgtaaggc cgacctcagc tttgccaatc tgaccttcgc cgatctcagc gatgcaaagc
     1681 ttgcggacgc aaggctttgg aaagccgacc tcacagcggc gaaactcgtt gccgccgatc
     1741 tgtcgggcgc aaacctgacg atggcgacat tgagagaaac cgacctctcc ggcgcaaatc
     1801 tccagggggc cgagctcatt tatgccatcc tggtcgaaac aaatctcaag cagaccaatt
     1861 tacaaggctg ttttatctag gggctttccg cctgggatgt gcaactcgaa ggggcaaatc
     1921 agacggacct
//
LOCUS       DASZAK010000333         4374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_612674, whole genome shotgun sequence.
ACCESSION   DASZAK010000333 DASZAK010000000
VERSION     DASZAK010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_612674"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1536)
                     /locus_tag="VGT44_05885"
     CDS             complement(202..1536)
                     /locus_tag="VGT44_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2580365.1"
                     /translation="MSSIERFIADARQLLPTMPITGEPEQLRDYTIDGVMPRLVVTPG
                     TKEETAQAVNLAGQHGLTLLARGGGSRLNLGGIPGQVDILLETNRMTRLIEHEAPDLT
                     CQVEAGITLAALQAQLATKGQRVALDPPDAEQASLGGILASNASGPKRLRYGTARDVV
                     IGLRVVQANGEIARSGGNVVKNVAGYDLNKLYIGSLGALGIIVEANLKLQPLPVAERT
                     LLLTYPNADDAMQTVTALLQSQLTPSAIELIDAGAASDMTDFFGLDLPTEGYTLAIDF
                     EGSPTAITRQLDETRLLARKAGALMGDDLDGPAQAQLWQAVREHMRGTVTCKVALLVS
                     HVGPFLTTINTVCRAHRLEAAVIAHAGNGVLYVELRPGDAMPRLAEGIAELRRQAQEA
                     RGSLVIERCPIELKRRIDVWGEPGGEFYLMQRLKQQFDPKSTFVQGRYLGGL"
     gene            complement(1580..3043)
                     /locus_tag="VGT44_05890"
     CDS             complement(1580..3043)
                     /locus_tag="VGT44_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2580366.1"
                     /translation="MSITELPTLTTPLYERMSAAKKAEVVRELESIIGARYVLYTPYD
                     LMLYEYDASIDRCTPDVVVLPSSTEEVAEIVKIAARHHVPVVPRGAGTGLSGGAIPIY
                     GGIVIAFARMNRILEVDYDNLRAVVQPGLVNLHLSNALNPQGFYYVPDPSSQRSCTIG
                     GNVGENAGGPHTLIYGVTTNHVLGLEIVTPEGKVVQVGGWTQDTPGYDLVGLLIGSEG
                     TLCIVTKIIVRIVHLAEAVRLMVGVFDTMDDASNTVSEIIASGVIPAAIEMMDQMILQ
                     AVEADTHAGYPMDAAAVLLIETEGLKESVEEQSATIVGICNKHHARVVKVAANEAERQ
                     LYWAGRKNAFGAVGRISPEFYVQDGVVPRTKLPYVLRRVGEICDSYGLRVGNVFHAGD
                     GNLHPLILFDSLIPGEVERVRKAGHDILSVCAEVGGSITGEHGVGIEKQEEMALIFGE
                     VDLRIMQQVREAWNPEQLFNPGKLFPMPGRCADMKQL"
     gene            3372..3878
                     /locus_tag="VGT44_05895"
     CDS             3372..3878
                     /locus_tag="VGT44_05895"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEV2580367.1"
                     /translation="MEAVDTYSRLIDLLDQHEVSYQLIDHIPEGRTEVISPIRGNALS
                     QAAKCIILMVKIGKKVTRYILAVVPGDRRVDLQAVKAMMGATYVAFASQDIAERLAGS
                     VAGTVLPFSFNPELELVVDPALLENSELYFNAARLDRSMVLKTSDYVALAKPRMGRIA
                     GVPGVNHL"
BASE COUNT          860 a         1324 c         1306 g          884 t
ORIGIN      
        1 cgaagatggg acgcgcatcg gcggcccctc gctgcaggcg ggcaacgtcg ggcgcgctgc
       61 acagggcaat gccgggagcc agcagcgctc cgctccgcac ctgcggatcg aggcgtttga
      121 gttcgcgcac cgccgcctct aaaaattcgg tttgcgcggt gagaataaat tgcgtcaaag
      181 atgtatcatg cgtgtccagc ttcacaatcc ccctaaatag cgaccctgca caaacgtgct
      241 tttcggatca aattgttgtt tgaggcgctg catcaaatag aactcccctc ccggctcgcc
      301 ccatacatcg atgcgccgtt tcagttcgat ggggcagcgt tcgatcacca ggctgccgcg
      361 cgcttcctgc gcctgccgac gcagctcggc aataccctcc gccagtcgag gcatggcgtc
      421 gcctgggcgc agctcaacgt aaaggacgcc gttcccggcg tgagcgatta cggctgcttc
      481 gagacgatgg gcgcggcaga ccgtattaat agttgtaagg aacggaccga cgtgtgacac
      541 aagcagcgct actttgcagg tgaccgtgcc gcgcatatgc tcgcgtaccg cctgccagag
      601 ctgcgcctgc gccgggccgt ccaggtcgtc gcccatcagc gcgccggctt tgcgcgccag
      661 caggcgcgtt tcgtccaact gccgggtgat ggcggtcggg ctgccctcga agtcgatggc
      721 gagcgtatac ccctcggttg gcaggtccaa tccgaagaaa tcggtcatat cgctggccgc
      781 gcccgcgtcg atcagctcga tggcgctggg tgtgagctgg gactgaagca gcgccgtgac
      841 ggtttgcatc gcgtcgtcgg cgtttggata ggtgagcagc agtgtgcgct cggcgacggg
      901 tagtggttgc agcttcaggt tggcttcgac gatgatgcca agcgccccca gcgagccgat
      961 atagagctta ttgaggtcgt agcccgccac gtttttcacg acgttgccgc cgctgcgcgc
     1021 tatttctccg ttggcctgca cgacgcgcag cccgattacc acgtcgcgcg ccgtgccgta
     1081 gcgaaggcgt ttggggccgc tggcattcga ggcgaggatg ccgcccagcg aggcttgttc
     1141 cgcgtcgggc ggatcgagcg cgacccgctg ccccttcgtc gccagttgtg cctggagcgc
     1201 tgccagcgtg atgcccgcct cgacctggca ggtgagatcg ggcgcttcat gttcgatcag
     1261 ccgcgtcatg cgattggttt cgagcagaat atcgacctgg ccagggatgc cgcccaggtt
     1321 gaggcgagag ccgccgccgc gcgccagcag cgtcaaccca tgctggccgg ccaggttgac
     1381 agcctgcgca gtctcttctt tcgtgccggg agtcaccacc aggcgcggca tgacgccatc
     1441 gatggtgtaa tcacgcagtt gttccggttc gcccgtgatt ggcatggtgg gcaggagctg
     1501 ccgcgcatcg gcgataaaac gttcaatact gctcatgaaa gcttcttgat cctcccagga
     1561 ctatctattg tagtcgcgtt tagagttgtt tcatgtcggc gcaccgaccg ggcataggga
     1621 agagtttgcc aggattgaaa agctgctctg gattccacgc ttcgcgcacc tgctgcatga
     1681 tgcgcaggtc aacctcgcca aaaatgagcg ccatctcttc ctgcttttcg atgccgacgc
     1741 cgtgttcgcc cgtgatagag ccgccgacct ccgcgcacac cgacaggata tcatgtccgg
     1801 ccttgcgcac gcgctcaacc tcgcccggaa tgagcgaatc gaagaggatc agtgggtgca
     1861 ggttgccgtc gccggcgtgg aagacgttgc ctacgcgcaa gccgtagctg tcgcagatct
     1921 cgccgacgcg ccgcagcacg tagggcagct ttgtgcgcgg caccacgcca tcctgcacgt
     1981 agaattccgg gctgatacgt ccgaccgcgc cgaaggcatt cttgcgcccg gcccagtaga
     2041 gctggcgttc cgcttcgttg gcggcgacct tcaccacgcg cgcgtgatgc ttgttgcaaa
     2101 tgccgacgat ggtggccgat tgctcttcga cgctctcttt caatccctcc gtctcgatca
     2161 gcagcacggc ggcggcatcc atggggtagc ccgcgtgtgt atcggcctcg accgcctgca
     2221 agatcatctg gtccatcatc tcgatggcgg cgggaataac gccggacgca atgatctccg
     2281 agacggtatt cgaggcgtca tccatcgtgt cgaagacgcc caccatcagg cgcacagctt
     2341 ccgccaggtg gacgatgcgc acgatgattt tggtgacgat gcacagcgtc ccctcggagc
     2401 cgatcagcag acccaccagg tcgtatcccg gcgtgtcttg cgtccagcct cccacctgga
     2461 cgaccttgcc ttctggcgtg acgatttcca ggccaagcac atgattggtg gtgacgccgt
     2521 agatcagcgt gtgcggccct ccggcgttct cgcccacgtt gccgccgatg gtacaggaac
     2581 gctggctgct gggatcgggt acgtaataga agccctgcgg attgagggcg ttgctgaggt
     2641 gcaggttgac cagccccggc tgcacaaccg cgcgcaggtt gtcgtaatcc acctccagga
     2701 tgcggttcat acgagcaaat gcgatcacga taccgccata aataggaata gcgccgccgc
     2761 tcaacccggt acccgcgccg cgaggcacga ccggcacatg gtggcgagct gcgatcttga
     2821 cgatttcggc cacctcttcc gtcgatgatg gcagaacgac cacgtccggt gtgcagcggt
     2881 ctatcgaggc gtcgtactcg tagagcatca ggtcataggg cgtgtagagt acgtaacgcg
     2941 ctccaatgat cgattccagc tcgcgtacca cctcggcctt ctttgccgca gacatgcgtt
     3001 catacagtgg cgtcgtcaag gttggaagtt cggttatgct catggctctg atccttcagt
     3061 cttttggctg cttgccagca cgcgctagat caacgggcgt catacttaga taataccatc
     3121 tagaagaacc aaatgaaagc ggctaatttc tgccggatgg aacccgttgt aggggcgcaa
     3181 tgaattgcgc cctcgtccct tgcttgctaa gcgcctccac cagggcgcaa ttcattgcgc
     3241 ccctacaaca gcttcctaca tcctgtgcta ctccctgtca gaaatgacag tagcagaagt
     3301 cagccattta tggcaaaatc cttgaacgca gacaattttt taccaaaacc cgatttgaaa
     3361 ggacatcttg tatggaagct gtagatacct actcgcgcct gatcgacttg ctcgatcaac
     3421 acgaggtatc ttaccaattg atcgaccata tccccgaagg gcgcacggaa gtaataagtc
     3481 cgattcgcgg caacgctctt tctcaggcag caaagtgcat cattctgatg gtcaagattg
     3541 gtaaaaaagt cacgagatat atactggctg tcgtcccagg cgatagaaga gtcgatttgc
     3601 aggcggtcaa ggctatgatg ggagcgactt acgtggcctt tgcctcccag gatatcgcgg
     3661 aacgactcgc gggcagtgta gcggggacag ttctgccctt ctccttcaac ccggagctag
     3721 aactggtcgt tgatcctgca ctacttgaga atagcgaact ctatttcaac gccgcgcgcc
     3781 tggaccgctc gatggtgttg aaaaccagcg actatgttgc actagcaaag ccgcgcatgg
     3841 ggcgcattgc cggggtacca ggcgtgaatc atctgtaggg gcgagccttt atggtcgccc
     3901 acccgcacat gcccttcgac ggaatcagcc tccagtgcgg ccaccggagc agggaggacg
     3961 agggtgacga cagaataata ccgccaccgg agcagggaaa acgagggcga ccgcaagggt
     4021 ctccacccca ctcccttccg cccccgccct tacaatgact acgaggccgc tcgacaggct
     4081 aattcgtcat cattgtaagg gcgggggtgg agtggagtgg ggtggagacc ctggcggtcg
     4141 ccctggggct tatacaagtg acagattata tagccaccac acaagcggct cttgattcat
     4201 cacgacgaat acaccttcca gcaccagaag agcgaccccg caagcaaaac aatcagcgct
     4261 gtcaacagaa tgcggcgctg cacattcgcg ccaccgctgc taggcgaggg cgtattcacc
     4321 agaggcggat tgacgccaac aggcgtgggg gttgcggctg ccggtgttga cggg
//
LOCUS       DASZAK010000334         4867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_683313, whole genome shotgun sequence.
ACCESSION   DASZAK010000334 DASZAK010000000
VERSION     DASZAK010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683313"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1069
                     /locus_tag="VGT44_05900"
     CDS             308..1069
                     /locus_tag="VGT44_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2580368.1"
                     /translation="MSGHSKWAQIRRSKGVNDARRGQLFTRLGREIVVAAREGGSGDT
                     NANFRLRMAVQRARDANMPMDNIERTIKRAMGGGEGMNLEEITYEGYGPGGTAMIVQT
                     LTENRNRTVAEVRNAFSRNGGNLGENGCVDWLFENKGIVEVELKGHDPDELSLEAIDL
                     GADDVDPAGADDETLVIYTDPSEVDKVRQALESKKYHVTKAESSLLPKTKIDLSDEKT
                     AHQVMRLVEKLEDLDDVQNVYTNADFPEEFAASYE"
     gene            1078..1602
                     /gene="ruvC"
                     /locus_tag="VGT44_05905"
     CDS             1078..1602
                     /gene="ruvC"
                     /locus_tag="VGT44_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEV2580369.1"
                     /translation="MSTVPDIDSQPGMRIALGIDPGTAIVGYAVVAARGSELNLLACD
                     VITTAAGTPLPQRLQVIYKRLSEVIAAYQPHESAMEELFFAKNARTAITVGQARGVAM
                     LALANGGLPIAEYTPMQVKQAVTGYGGAKKEQVGEMVRILLRLKAVPHPDDAADAAAI
                     AICHLHMAPLLTIR"
     gene            1681..2271
                     /gene="ruvA"
                     /locus_tag="VGT44_05910"
     CDS             1681..2271
                     /gene="ruvA"
                     /locus_tag="VGT44_05910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV2580370.1"
                     /translation="MIAGISGVLEARRADHALIRVGGFSLRVFAPATTLNRLGEPGAE
                     VSLHTHFQVREDGMALYGFSGEEDRDAFEKLITISGVGPKIALALLSSMDARTLFKTI
                     ADEDQQRLSMVSGVGKKLAAKLVLELKGKLPSLIALGGASALSGSGTMSEALEALMGL
                     GFSAAEAQAALLKLPQDRPLTLEEQITHALRSFARD"
     gene            2295..4739
                     /locus_tag="VGT44_05915"
     CDS             2295..4739
                     /locus_tag="VGT44_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580371.1"
                     /translation="MAIDEQLPPACAFAVSVLVERDMDPDAVPEEAVEAAQLHLATCV
                     RCLSSPPAISPPRKKKKRRPVEPEEGSYLQPYIPAPFEDTAPETWYPKLSEAPSGPTR
                     SSALAPMPLSLPTTPIPANAPVSAAPRPQESVQAIAPAKMATPAAQPSSTAAPASTRQ
                     PAAMDKPMNCAQCRQVLQEYAEAMDRGENVEMLFPEAQAHLYSCESGCLVLLDLFRQE
                     AKASRKYRRRPVRDPFSAIGWELSGFFRGGQVPMSPMALAYGTLVLLLIVASLSALLA
                     VRWDDARYYHPTNTVSLPTPDGIGLSDGLKVFDACNANSYQSKRQAAQAMQKQETTKA
                     EQLLTTATGAASADTSGCNGGESAIYQEDLLVRQSGHPYGALVVSFDSGPGVADPRGG
                     TDRHILYAAYTQELVGAYIAQQHYNQQAIQTPGMPLLYLILANTTGVEQGALQIANDI
                     SALAASNNSSSLQQFGLLASGPLPLLGVLGLAPSSLVQVVLPVLCRAGVPLIAPTATG
                     LFIINLLLQTSLYRHCAPGFSFTRFSPDDASQSYLAALYAYTHLHVRHAAIFYDPSNP
                     SSSDSADSFSTDFSKFRGAAIVAREAAVASGLQGTDGRPQASPDELQAGIQDALQAKP
                     RPDMIFAPLLTNDVITLAQNIARLPVNQQPILMIGGEFVHPTALQGLAQWARQQQLTL
                     PRIYVSLVSAARPPNNSNAWQEAFYASLCTSFAAPGSHCSGAGIIDQGGLLFADGTEL
                     VANALIPPPVKGSATSAFSIPTRATLSQRISRERFVGVSSPIRLHTTAGMLITSLQVA
                     PVILGVQEDGSLQIVG"
BASE COUNT         1014 a         1521 c         1419 g          913 t
ORIGIN      
        1 gggcgcattg cagcttcaga gagcaggcgc gtttatcacc tcactcgctg gtatggggct
       61 attctatcat agcggcggca gactgaatag ataggtacca cgtgaatgat ttcctgggat
      121 gtgagaccag gttcccggag ggcgtaccct tttggtcgcc ctccgggaac ctcggcagtc
      181 attggcgaat ccccctcgac tagcaacgcc gcttcatgtt acaatgcgta gagcatcata
      241 ctctcaagaa caccgaaagc catatttgtt gatacgtgtt ccctgaaaac gacacaggag
      301 ctgtgaaatg tcaggacatt caaagtgggc gcagatcagg cgctccaagg gcgtcaacga
      361 cgccaggaga ggacaactct tcactcgact agggcgtgaa attgtggtcg cggcccgcga
      421 aggaggcagc ggcgacacca acgcaaattt ccgcctgcgc atggcggtgc agcgcgcgcg
      481 cgacgccaat atgccgatgg ataatatcga acgcaccatc aagcgcgcca tgggcggcgg
      541 cgagggcatg aacctggaag agattaccta cgaagggtac gggccaggcg gtacggccat
      601 gatcgtgcag accttgaccg agaaccgcaa ccgcacggtc gccgaggtgc gcaacgcctt
      661 cagtcgcaac ggcggcaacc tgggtgaaaa cgggtgcgtc gactggttat ttgagaacaa
      721 aggcatcgtc gaggtcgaac tcaaagggca tgatcccgat gaactgtcgc ttgaagcaat
      781 cgacctgggc gcggacgatg tagacccggc gggcgcggac gacgaaacgc tcgtgattta
      841 caccgatcca tcggaagtag acaaagtgcg ccaggctttg gagtcgaaga aatatcacgt
      901 caccaaagcg gaaagctcgc ttctgcccaa gacgaagatt gatttgagcg acgagaagac
      961 cgcgcaccag gtcatgcgtc tggtagaaaa gctggaagac ctggatgacg tgcaaaacgt
     1021 gtataccaac gcggacttcc ctgaagaatt cgccgcgtcg tatgagtaat aaacactatg
     1081 agtacagtcc ccgatatcga tagccagccc ggtatgcgta tcgccctggg catcgatccg
     1141 gggacagcca tcgtgggcta cgcggtcgtt gccgccaggg gcagcgagct aaacctgctg
     1201 gcctgtgacg tcattacgac ggcggcaggc acaccactgc cccaacgcct gcaagtgatc
     1261 tacaaacgac tgagcgaggt aatcgccgcg tatcagccgc acgaatccgc gatggaagag
     1321 ctattcttcg cgaaaaacgc gcgcaccgcc atcacggtcg gccaggcgcg cggcgtggcg
     1381 atgctggcgc tggccaatgg cggtctcccc atcgcggaat atacgcccat gcaggtcaag
     1441 caggccgtta ccggttatgg cggcgcgaaa aaggagcagg tcggcgagat ggtgcgcatc
     1501 ctgctgcgcc tgaaggcggt cccacacccc gatgatgccg ccgacgcggc ggctatcgcc
     1561 atctgccatc tgcatatggc gccgctgctg acgatccggt aaaactcact atccatatct
     1621 cctactttga tgcgaattac cttactaaat gttaatctat agacgaggga aggcaaaata
     1681 atgattgccg gcatttctgg ggttctcgaa gcgcgtcgag cagatcatgc gcttatccgc
     1741 gttggcggct ttagcctgcg agtcttcgcg cccgccacga cgcttaatcg cctgggcgag
     1801 ccgggggcgg aggtttcgct gcacacccat ttccaggtgc gcgaggatgg catggcgctg
     1861 tatggcttta gcggggaaga ggatcgggac gccttcgaga agctgatcac cattagcggt
     1921 gtcgggccga agatcgccct ggcgctcttg tcgtcgatgg acgcgcgtac gttatttaag
     1981 accatcgccg acgaggacca gcagcgcctg tcgatggttt ctggtgtcgg caaaaagctc
     2041 gctgcaaagc tcgtgctgga attgaagggc aagctcccgt cgctgatcgc tctggggggt
     2101 gcgtcggcgt tgtctggctc cgggaccatg tccgaggcgc tggaagctct gatgggtctg
     2161 ggcttttctg ctgccgaggc tcaggctgct ctgctcaagc ttccacagga ccggccattg
     2221 acgctggaag agcaaatcac ccatgcctta cgctcctttg ctcgtgacta attctatgct
     2281 tgaggaggac gttgatggca atcgacgaac aactgccgcc agcgtgcgcg ttcgccgtct
     2341 ccgtgctggt cgagcgcgat atggaccccg atgctgtccc ggaagaggct gttgaggcgg
     2401 cgcaacttca tttggcaacc tgtgtgcgct gcctgagttc accgcccgcc atcagcccgc
     2461 cgcgcaagaa gaagaaacgc cgcccggtag aaccagaaga gggatcatac ctccaaccgt
     2521 atatacccgc gccatttgaa gatactgcgc cagaaacatg gtatccaaag ctatcagaag
     2581 cgccctcggg tcccacccgc tcctccgcgc tcgcgccgat gccgctctca ttgcctacca
     2641 cgccaatacc cgccaatgcg ccggtatctg ccgctcctcg cccgcaagag tcagttcagg
     2701 ccatcgctcc ggcgaaaatg gcgacgcccg ccgctcaacc atcatccact gcggctcctg
     2761 cttcgaccag gcagcccgcc gccatggaca agccgatgaa ttgcgcgcag tgccgccagg
     2821 tcttgcagga atacgccgag gcgatggatc gcggcgaaaa tgtggagatg ctcttcccgg
     2881 aggcacaggc gcacctgtat agctgcgaat cgggctgcct ggtactgctc gacctgtttc
     2941 gccaggaagc gaaggccagc cgcaaatatc gccgccgacc ggtgcgcgat ccattcagcg
     3001 ccatcggttg ggaactgagc ggcttcttcc ggggcggcca ggttcccatg tctcccatgg
     3061 cgctcgccta tggtacgctg gtactcctgc tcattgtggc ctcgctgagc gcgctgctcg
     3121 ccgttcgctg ggacgacgcg cgctactacc accccaccaa tacggtgtcg ctgcccacgc
     3181 ccgacggcat cggcttgagc gatggtctga aagtcttcga tgcctgtaac gccaacagct
     3241 atcagagcaa gcgccaggca gcccaggcaa tgcagaagca agaaacgaca aaggctgaac
     3301 aactgctgac cactgcgacc ggcgcagcca gcgccgacac ctccggctgc aatggcgggg
     3361 aatccgccat ctaccaggag gacctgctcg tgcgacaatc cggccatccc tacggcgcgc
     3421 tggtggtcag cttcgacagt ggccccggcg tggcagaccc gcgcggcggc accgaccggc
     3481 atattctcta tgccgcctat acgcaggagc tggtgggcgc ctatatcgcg cagcagcact
     3541 ataaccagca agccatccag acccccggta tgcccctgct ctacctcatc ctggcaaaca
     3601 caaccggcgt agagcaaggc gcgctgcaaa tcgcaaacga tatatccgcg ctggctgcga
     3661 gcaataactc cagtagcttg cagcagttcg ggctgctcgc cagcggcccg cttccattgc
     3721 tcggtgtgct gggactggcg ccgagcagcc tggtacaggt cgtcctgccc gtgctgtgcc
     3781 gggcgggcgt cccgctcatc gcgccgacgg ctacgggttt attcatcatc aacctgctct
     3841 tgcagacctc gctctatcgg cactgcgcgc ccggcttcag cttcacccgc ttttcgccgg
     3901 acgacgcatc tcagagctac cttgccgccc tttatgccta cactcacctg catgtgcgcc
     3961 acgccgcaat cttctacgat cccagcaatc cgtcctcgag cgattcggcg gatagctttt
     4021 cgactgattt ctcaaaattt agaggagctg cgatagtggc gcgtgaagct gcggtggcga
     4081 gtggcctgca aggcaccgat ggacgcccgc aggcatcgcc cgacgaacta caggcaggca
     4141 tccaggacgc gctgcaagct aagccgcgcc cggacatgat cttcgcgcca ctgctgacca
     4201 atgatgtgat tacgctggcg cagaatatcg cgcgcctgcc cgtcaaccag cagcctatct
     4261 taatgatcgg cggcgagttt gtgcatccaa ctgcgctaca agggttggca cagtgggcgc
     4321 gtcagcagca actgacgctg ccccgcatct acgtctcgct cgtctccgcc gcgcgcccgc
     4381 ccaacaattc caacgcctgg caggaagctt tctacgccag cctctgcacc tctttcgccg
     4441 ctcctggttc gcattgcagc ggcgcgggca tcatcgacca gggcggtctg ctcttcgctg
     4501 atggtaccga actggtagcc aacgcgctga tcccgccgcc tgtcaagggc agcgcgacga
     4561 gtgccttctc catcccgaca cgcgcaacct tatcgcagcg catcagtcgc gagcgcttcg
     4621 tcggcgtcag cagccccatc cggctgcaca ccactgccgg tatgctgata accagcctgc
     4681 aggtcgcgcc agtcattctc ggcgtgcaag aagacggcag cctgcaaatc gtcgggtgat
     4741 acgtggcgcg acaaatatca ggagataata tagagaagcg gcaccctatc atgaaaattt
     4801 agggtgccgc ttctcctata ctcaagacct tttcggtctg accacactac ggcaagtcat
     4861 ctggccg
//
LOCUS       DASZAK010000335         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_435101, whole genome shotgun sequence.
ACCESSION   DASZAK010000335 DASZAK010000000
VERSION     DASZAK010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435101"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="VGT44_05920"
     CDS             complement(<1..700)
                     /locus_tag="VGT44_05920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEV2580372.1"
                     /translation="MHRFFVEPALLTSQASPAIVSLPKDLAHQVRDVLHLQVNEQLIL
                     LDNSGDETLAAVTHISKAAVIVRLLERRAGKRESPVHIILCQGLLKSARFEWILEKGT
                     ELGVAAFSPILTRRSMAGLEEIGASKVQRWRRIIQEAAEQCGRARLPELLPVRPLIHA
                     LNDIPPGSLSLMPWEEEHALSLRDALTAFHSQPLQLSGQSLTVTLFIGPEGGLMAEEV
                     ALAQRHGAQAVSLGP"
     gene            complement(702..>1673)
                     /gene="prmA"
                     /locus_tag="VGT44_05925"
     CDS             complement(702..>1673)
                     /gene="prmA"
                     /locus_tag="VGT44_05925"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV2580373.1"
                     /translation="DPRGRHASAQSHYDRRKHHMRWLELTVQTHPEAIESVSELLTQH
                     TTGGVAIEEPIELIDEGQEYRVLAGQPVKVHAYVPLDGSEEQARQRIAEGLWHLSSLG
                     PHFVGELETRVVNEEDWANAWKDYFHVTHIGERLVIRPSWREYTPKAHEVVLELDPGM
                     AFGTGLHPTTRMCLEQVERVTRPGMRVLDVGTGSGILALAAAKLGASSVHCIDNSSVA
                     VESAAANAAANGLSERITVTLGTLDETEAVRMADQYDLVLANIIARVIGSIAPQLAQT
                     LAPGGLLIASGIIEERRHEAEQPLLATGLTLVEQVMIDDWVTLVMSK"
BASE COUNT          268 a          482 c          579 g          344 t
ORIGIN      
        1 gcggtcccag cgagacggcc tgcgcgccgt gccgctgtgc cagcgcgacc tcctcggcca
       61 tcaaccctcc ttccgggccg atgaagagtg tgacggtgag ggactggccg gataactgga
      121 gaggttgaga atggaaggcg gtcagggcgt cgcgcagact cagtgcgtgt tcctcttccc
      181 agggcatcag ggagagcgag ccgggaggga tatcgttgag ggcgtgtatg agagggcgca
      241 ccggcagaag ttcgggcagg cgcgcgcgcc cgcattgttc ggcggcttcc tggataatgc
      301 gccgccagcg ctggactttt gacgcgccga tttcttcgag gccggccatc gagcggcgtg
      361 tgaggatggg cgaaaaggcg gctacgccca gttctgtgcc tttttccagt atccattcaa
      421 agcgcgcgga tttgagcaag ccctggcaga ggatgatatg caccggcgat tcgcgtttcc
      481 cggcgcggcg ctcaagcagg cgaactatga cggccgcctt gctgatgtgc gtgacggcgg
      541 ccagggtctc gtccccgcta ttgtcgagca agatcaactg ctcattgacc tggaggtgca
      601 gcacgtcccg cacctggtgg gcgaggtctt tggggagcga gacgatggcg ggggatgcct
      661 ggctcgtcaa taacgctggt tcgacgaaaa agcggtgcat cttatttgct cattaccagt
      721 gtgacccaat cgtcgatcat gacctgttct accagggtga gcccggtggc cagcaggggc
      781 tgctcggctt cgtggcggcg ttcctcgatg atgccgctgg cgatgagcag gccgccgggt
      841 gcaagcgttt gggcgagttg gggggcgatg gaaccgatga cgcgcgcgat gatatttgcc
      901 agcacgaggt cgtactggtc ggccatgcgc actgcctctg tctcatccag cgttccaagt
      961 gtgacggtga tgcgctcgct caggccgttg gcggcggcgt tggcggcggc gctttcgacg
     1021 gcgacgctgc tgttgtcgat gcagtggacg cttgacgcgc cgagttttgc cgcggcgagg
     1081 gccaggatgc ctgagccggt gccgacatcg aggacgcgca taccgggtct ggtgacgcgc
     1141 tcgacctgtt cgaggcacat acgcgtggtg gggtgcaggc ccgtgccgaa ggccatgccg
     1201 ggatcgagtt ccagcacgac ctcgtgcgcc ttcggggtgt attcccgcca ggatggacga
     1261 atgacgagcc tttctccgat gtgcgtgacg tgaaaatagt ctttccaggc attagcccag
     1321 tcttcctcgt tgacgacgcg cgtctccagt tcgccgacga agtgcggccc caggctggag
     1381 aggtgccaca gcccttcggc gatacgttgg cgcgcctgtt cttcgctgcc gtccagcggg
     1441 acgtaggcgt gaaccttgac cggctggccc gccaggacgc gatactcctg gccctcgtcg
     1501 atcaattcaa tcggttcctc tatggcgact ccacccgtcg tgtgctgggt cagcagttcg
     1561 ctgaccgact cgatggcctc tgggtgggtt tgtacggtta attctaacca gcgcatatga
     1621 tgcttccttc tgtcgtagtg tgactgcgcg ctggcatggc gaccgcgagg gtc
//
LOCUS       DASZAK010000336         2374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_150530, whole genome shotgun sequence.
ACCESSION   DASZAK010000336 DASZAK010000000
VERSION     DASZAK010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150530"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1891)
                     /gene="purM"
                     /locus_tag="VGT44_05930"
     CDS             complement(32..1891)
                     /gene="purM"
                     /locus_tag="VGT44_05930"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV2580374.1"
                     /translation="MTIDAIPRALVLRAPGINCDRETAHACRVAGFETELVHINRLIQ
                     DPARLLDYHLLVIPGGFSYGDDLGAGTLLAKNLEIHLGEQLRQFIDEGRLVLGICNGF
                     QALVRAGLLPGSTTHYDERVVATLTDNASAQFECRWVTLATQDSPCIFTQGIDHPIEL
                     PVAHGEGQFALADLAMLEQLQANKRVPIVYTITAWQASRKSPSPATPPFSSFAADVLY
                     PDNPNGSLGNIAGICNERGNVFGLMPHPERYVQALQHPQRRGQEGMGGGLLMFKNAYR
                     YVKDVFCRDAYAASGVDIAAADAAKTLMRAAVQATQGPEVLAGMGAFAGVFDLSAVKD
                     MRRPALVASTDGVGTKTLIAAQAERYDTIGYDLVNHSVNDLLTQGARPLFFMDYLAMG
                     KLDGQQAADIVRGVANACREVGCALLGGETAEMPGVYLPGAFDLAGTIVGVIDWDTPL
                     RPAAMQPGDVLSGLPSSGLHTNGYSLARRVFAPYALETVFPELGEPLVDALLRPHRGY
                     LREMTMLRERLGAGMKGAAHITGGGFEGNISRILPEGIQAVIETNGWDAPPLFGLIQR
                     LGHISRNEMFRAFNMGIGMVLVFSPEAAQEARRLLPELLAVGYLTKGAGVVLS"
     gene            complement(1888..>2374)
                     /locus_tag="VGT44_05935"
     CDS             complement(1888..>2374)
                     /locus_tag="VGT44_05935"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEV2580375.1"
                     /translation="RYRSGDPRGRHANARHAPRVDITTARATMQAVGSAVREGLARAC
                     HDLSEGGLAVAAAEMSLAGLLGVSLDLEAVPREPLELEAEQAQSVALFSETASRFLVE
                     VTPGQQAAFEQHMRALGINDMALIGHVTHGGQFVVRDGTRVVIDVAVETLQAAWKGER
                     A"
BASE COUNT          454 a          784 c          696 g          440 t
ORIGIN      
        1 taaatcggcg gtgcgcgcgg taaaccggcg cctacgacag tactacaccg gctcctttcg
       61 taagataccc taccgcgagc aattcgggca acagacgtct tgcctcttgt gccgcttcgg
      121 gcgagaagac cagcaccatg cctatcccca tattaaacgc gcggaacatc tcattccgtg
      181 agatatgccc caggcgttgg atcaatccaa acagcggcgg cgcgtcccag ccgttcgtct
      241 caatgacggc ctggatacct tccggcagga tgcgcgagat attgccctcg aatccgccac
      301 cggtgatgtg cgctgcgccc ttcatacccg cgcccaggcg ttcgcgtagc atcgtcattt
      361 cgcgcaggta gcctcgatgc gggcgcagca gcgcgtccac aagcggctcc cccagttcgg
      421 gaaagaccgt ctccagcgcg tagggcgcga agactcgccg cgccagcgaa tagccgttgg
      481 tgtgcaggcc gctggatggc agccctgaca gcacatcacc cggctgcatc gcggcgggac
      541 gtagcggtgt atcccaatcg atgacgccga cgatcgttcc ggccaggtcg aacgcgccgg
      601 gcagataaac accgggcatc tccgccgtct cgccgcccag cagagcgcag ccgacctcgc
      661 ggcaggcatt cgccacgccg cgcacgatat cagcggcctg ctgcccatcc agcttgccca
      721 tcgcgaggta gtccatgaaa aagagcggtc ttgctccctg tgtcaacagg tcgttgacgg
      781 aatgattgac caggtcgtag ccgatggtat cgtagcgctc ggcctgcgcc gcgatcagcg
      841 tcttcgtgcc aaccccgtcc gtcgaggcga ccagtgcggg cctgcgcata tctttcactg
      901 cgctcagatc gaagacgcct gcgaacgcgc ccatgcctgc cagtacctcc ggcccctgcg
      961 tcgcctgcac tgccgcgcgc atcaatgttt tcgcggcgtc tgcggcggcg atatccaccc
     1021 cgctcgccgc gtaagcatcg cggcaaaaga catctttcac gtaccggtaa gcgttcttga
     1081 acatcaacag gccaccgccc atcccttcct gaccgcgccg ctgtggatgt tgcagcgcct
     1141 gtacgtagcg ttcgggatgg ggcatcaggc caaagacgtt gccgcgctcg ttacagatac
     1201 cggcaatatt gccgagggag ccatttgggt tatcaggata gaggacatcg gcagcgaatg
     1261 aggagaaagg cggtgtggca ggcgagggtg acttccgtga tgcctgccac gcagtaatag
     1321 tatacactat tggaacgcgc ttgttcgctt gcaactgctc aagcatcgcc aaatctgcca
     1381 gcgcgaactg tccttcgccg tgcgcaacgg gcaattcgat ggggtgatcg ataccttgcg
     1441 tgaagatgca cgggctatcc tgcgttgcca gcgtcaccca ccggcattca aattgggccg
     1501 aggcattgtc ggtcagcgtc gcaaccactc gttcgtcata atgtgtcgtt gagccgggca
     1561 gcaatccggc gcgcaccagc gcctggaagc cgttgcagat gcccagcaca agccgtcctt
     1621 catcgatgaa ttgtctgagt tgctcgccca ggtggatctc caggtttttc gccagcagcg
     1681 tacccgctcc caggtcatcg ccataggaga agcctccggg gatgaccagc aggtgataat
     1741 cgagcaggcg cgcggggtcc tggatcagcc ggttgatatg cacgagttcc gtctcgaagc
     1801 cagccacgcg gcaggcatgt gcggtttcgc ggtcgcagtt gatgcccggc gcgcgcagca
     1861 ccagggcgcg tggtatggcg tctatcgtca cgctcgttcc cctttccatg ccgcctgcaa
     1921 tgtctcaacc gcgacatcga tgacaaccct cgttccatcc cggaccacaa attgcccgcc
     1981 atgcgtcaca tgcccgatca gcgccatatc gttgatgccc agcgcccgca tatgctgctc
     2041 aaaggccgct tgctgtccag gcgtgacttc gaccaggaaa cgcgaggccg tctcactgaa
     2101 aagcgccacg ctttgcgcct gttcggcttc cagttctagt ggctcgcgcg gaacagcctc
     2161 caggtcaagc gagacgccca acagtcccgc cagtgacatc tcggcagccg ctaccgccag
     2221 cccgccctcc gaaagatcgt gacaggcgcg cgccaaccct tcacgcacgg cgctgccgac
     2281 ggcctgcatg gtagcgcgcg ctgtagtgat atcgacacgc ggggcatggc gagcgttggc
     2341 atggcgaccg cgagggtcgc cactacgata acga
//
LOCUS       DASZAK010000337         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_291809, whole genome shotgun sequence.
ACCESSION   DASZAK010000337 DASZAK010000000
VERSION     DASZAK010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291809"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..890
                     /locus_tag="VGT44_05940"
     CDS             168..890
                     /locus_tag="VGT44_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2580376.1"
                     /translation="MTEGISRPVSPISAQPLEAAVTSAPLAGYSASADEIRLVEALRN
                     GSETAFVMLIEQYHRALLRLAMVYVSSRAVAEEVVQETWLGVLQGLQRFEGRSSLKTW
                     IFRILTNCAKTRALREGRSVPFSSLAEFREEDDEPAVEPERFRTADMGSAGQWLTPPQ
                     RWDEIPEERLLSQETRARLWKAIEVLPPNQRAVITLRDIEGWDSEEICRFLDISEVNQ
                     RVLLHRARARVRRALEMYFDEE"
     gene            898..1161
                     /locus_tag="VGT44_05945"
     CDS             898..1161
                     /locus_tag="VGT44_05945"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV2580377.1"
                     /translation="MTNASEPTHALTCQEVVELVTDYLEQTLLPQTLAVFQAHLAECD
                     DCTTYTNQIQQTITMLRNLAQEPVFPTSRQELLTMFQSWKSGK"
     gene            1176..3092
                     /locus_tag="VGT44_05950"
     CDS             1176..3092
                     /locus_tag="VGT44_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV2580378.1"
                     /translation="MPLDYDIRSLGEALNRISRKLHEHTSKLEVAVEGTYAGESGDPR
                     VRLLSDSLRISRDADLELSRLNESAKRVILTLRDQLEQSSQPALASPLPSFPPSSTAI
                     GTLEREREALNTSLITLKEQHGELETLYEIARYLNSTLDFNKVLTEVMDQVIRVVKAE
                     RGFLMLVNERTKEHTFAIARDNKHNTLGKNEFNISMRTIDEVVRLGEARLADNAQEEL
                     KGHESIVAHGIRSIICAPLKVRDVCIGAVYVDSRITAKLFTEKHRDLMLAFCNQAAIA
                     INNARLFEDLNRALRKVEEDRQYMDNIFASIANGVITTDSAGSITKFNDAAGLILRIN
                     PMSAIGRHYRDVFRHLPQLGIVEMLQHVPKEHDHGTIVPRPLECEIPGRGPGTIHLTL
                     YASALRDGTTPIGTALVLDDRTEQKRAEASAKNIRRLFGRYVHPNVVQQLIDNPNAVN
                     LGGEVKEISVISTDIRGFTRLSERLPSREVMNLLNTYYEIMVKAIWDEEGTVTGFWGD
                     AMLAIFNAPLSQPDHALRAVRAAWNMRQAVLAFQKLQPPDLPIAFGIGVNTGEVIVGN
                     VGSQDRIQNYTAIGDTVNVAARLQENASDNNIILNHRTFSRVRQQVRVEKLPSLMVKN
                     KTGSLDVWRLVGLL"
     gene            complement(3089..>3987)
                     /locus_tag="VGT44_05955"
     CDS             complement(3089..>3987)
                     /locus_tag="VGT44_05955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2580379.1"
                     /translation="CGRHATSPDGASMASSKQARLLTMDDLPYLMRLLRTSEYVYQRF
                     TLDELPPLIQHCPAVGIMSNTTLQGFLLTQTVNPPSAWIGGYGVNWTESRAYVTLLDN
                     MLDALIPHLTRRGVRYLHYSGNDMHDDWLRDILLTRGFRPFRLLYAYDKYDFKIPSTG
                     NQQVVVRPFERRDLPAVLRVEEACFEDFWRYDARSFEDVAATHPYFVVAELNGNVIGY
                     QYNALDSDSGYLVRIAVHPSASGKAIGTRLMCEAVNFFKRARVLRIMLNTQDDNAHAH
                     RLYEWFGFVRVPQVGFVLRRDL"
BASE COUNT          919 a         1137 c         1151 g          780 t
ORIGIN      
        1 acataaagtg taacaattcc ccaggaagtg agactgtaca tatgccgtag ttgcgcacgc
       61 gccttgacga agccggattg ctgggatata ctccaatgcg gttgatacac ttcaacgtgg
      121 tagtatccgc agccctggaa acccgaacaa tgagaaggca ggggtcaatg accgaaggaa
      181 tttcccggcc cgtcagccct atttccgcgc aaccgttaga agcagcagtc acaagcgcac
      241 ctctagcggg ttatagcgcg tcggcggacg agatacgcct ggtcgaggca ttgcgcaacg
      301 gcagcgaaac ggcgtttgtg atgctcatcg agcagtatca tcgcgcgctg ctgcgcctgg
      361 caatggtcta tgtgtcgtca agggcggtgg ccgaggaggt tgtccaggag acctggttgg
      421 gtgtgttgca aggtttgcag cgtttcgagg ggcgctcgtc cctcaaaacg tggattttcc
      481 gcatcctcac caattgcgcc aaaacccgcg cgctgcgcga agggcgcagc gtcccgttct
      541 cgtcgctggc cgaatttcgg gaggaggacg acgagcctgc ggtcgaaccg gagcgattca
      601 ggacggcgga catgggatcg gcgggccaat ggctcactcc gccgcagcgc tgggatgaga
      661 taccggagga acgcctgctt tcgcaagaaa ctcgcgcgcg actctggaag gccatcgagg
      721 tcttaccgcc gaatcaacgc gcggtgatta cgttacgcga tatagaaggc tgggactcgg
      781 aagagatctg tcgcttcctc gacatttccg aggtgaacca gcgagtgctg ttgcaccggg
      841 cgcgcgccag agtgcggcgc gcgcttgaaa tgtactttga tgaagagtag gttcagcatg
      901 acgaacgcat cggagccaac gcacgcattg acttgccagg aggtggtgga gctggtgacg
      961 gattatctcg agcaaacctt gctgccgcaa accctggctg tgttccaggc acacctcgcc
     1021 gaatgcgacg actgtacaac ctacacaaac cagatacagc aaacaattac gatgctgcgc
     1081 aatttggcac aagagcctgt gttcccaaca agcaggcagg agcttcttac tatgttccag
     1141 tcgtggaaat caggaaagta ggatggaggg ataacgtgcc tctcgattat gatatacgct
     1201 cgctaggtga ggcgctgaac cgcatttcgc gcaagttaca cgagcataca agcaagctgg
     1261 aagtcgccgt cgaggggacc tacgcgggcg aaagtggcga tcctcgcgtt cgcttactca
     1321 gcgattcgct gcgcatttcg cgcgatgccg acctggaatt gagcaggttg aacgagtcgg
     1381 ccaagcgcgt gatcctcacc ctgcgcgacc aacttgaaca atcctcgcag cctgctctgg
     1441 ctagccccct tccatccttc ccgccctcat caactgctat cggcactctt gagcgcgagc
     1501 gcgaagccct gaataccagc ctcatcaccc tcaaagagca gcacggagag ctggagacgc
     1561 tgtacgaaat cgcgcgctat ctcaactcaa cgctcgactt caataaagtg ttgaccgagg
     1621 tgatggacca ggtgattcgc gttgtgaagg cggaacgcgg ctttttgatg ctggtcaacg
     1681 agcgcacaaa ggaacacacc tttgccatcg ctcgcgataa caaacacaat accctgggta
     1741 aaaacgaatt caacatcagc atgaggacga tcgacgaggt ggtgcgcctg ggcgaagcgc
     1801 gactcgctga taatgctcag gaggaattga aagggcatga gagtatcgtc gcgcatggca
     1861 ttcgttccat catctgcgcg ccattgaaag tgcgcgatgt ctgtatcggg gccgtctacg
     1921 tcgatagccg catcaccgcc aaactgttca ctgagaagca tcgcgacctg atgctggcat
     1981 tctgtaatca agccgcgatc gccattaaca acgcgcgtct gttcgaggat ttgaaccgcg
     2041 cgctgcgcaa ggtggaggaa gaccgccagt acatggataa catcttcgcc tctatcgcga
     2101 atggcgtgat taccaccgat tctgctggca gtatcaccaa attcaatgac gctgccgggt
     2161 taatcctgcg cattaatccg atgtccgcca tcggcaggca ctatcgcgat gtcttccggc
     2221 acctgcccca actcggcatc gtcgagatgc tccagcacgt gccaaaggaa cacgatcatg
     2281 gcaccatcgt tcctcggcca ttagagtgcg agatccccgg tcgcggacct ggaacgattc
     2341 acctgacgtt gtacgcctcg gctctgcgcg atgggacgac accgataggc acggcgttag
     2401 tactggatga tcgcaccgaa cagaaacgcg ccgaggccag cgccaaaaat atccgccgtc
     2461 tcttcgggcg atacgtccac cccaatgtgg tgcagcaatt gatcgataac cccaatgctg
     2521 tcaacctggg cggcgaggtg aaggagatat cggtaatctc aaccgatatt cgcggcttca
     2581 cacgcctgag cgagcgcctt ccatccagag aggtcatgaa cctgctcaac acctactacg
     2641 aaatcatggt gaaggccatc tgggacgaag agggaacggt cactggcttc tggggagatg
     2701 cgatgctggc gatcttcaac gcgcctcttt cgcagccaga tcacgcgctg cgagcggtgc
     2761 gggctgcctg gaatatgcgc caggcagtgt tggcatttca aaagttgcag cctcccgatt
     2821 tgcccatcgc gttcggcatc ggggtcaaca ccggcgaggt catcgtcgga aacgtcggtt
     2881 cgcaggatcg tatccagaac tatactgcca tcggcgatac cgtcaacgta gcggcgcgcc
     2941 tgcaagagaa tgcctctgac aacaatatca ttctgaatca ccgcaccttc tcgcgcgtgc
     3001 gccagcaggt gcgcgttgaa aagctgccat cgctcatggt gaaaaacaag accggctcgc
     3061 tcgatgtctg gcgcctggtc ggcttactct aaaggtcccg gcgcaagaca aaaccgacct
     3121 gtgggacgcg cacgaaacca aaccattcgt acagccggtg cgcgtgtgcg ttatcgtcct
     3181 gggtgttgag catgatgcgc aggacgcgcg cgcgtttgaa aaagttcacc gcctcgcaca
     3241 tcaggcgcgt gccgatggcc ttgccgctcg ccgagggatg caccgcgatg cgcaccaggt
     3301 agcccgagtc gctatccagc gcgttgtact ggtagccaat gacgttgccg ttcagttcgg
     3361 ccacgacaaa atagggatgc gtcgcggcga catcttcaaa cgagcgcgca tcgtagcgcc
     3421 agaaatcctc aaagcacgcc tcttcaaccc tcaataccgc cggcaggtcg cgccgctcaa
     3481 acgggcgaac aaccacctgc tgattgccgg tcgaagggat cttaaaatcg tatttgtcgt
     3541 aagcatacag caggcgaaat ggtcgaaagc cgcgcgtcag caggatatcg cgcagccagt
     3601 catcgtgcat atcgttgccg gaataatgca ggtagcgcac accgcgcctg gtcaggtggg
     3661 ggatcagcgc atcgagcatg ttatccagca gggtgacata ggcacggctc tccgtccagt
     3721 tcacaccata cccgccgatc caggcagagg gaggattgac cgtctgcgta agcaggaagc
     3781 cctgtaacgt cgtattgctc atgatgccta ccgccgggca atgttgtatc agcgggggca
     3841 gttcatccag ggtaaagcgt tgatagacgt attcagaggt acgaagcagg cgcatgaggt
     3901 agggcagatc atccattgtc aacaatcgcg cttgcttaga actggccata ctcgctccat
     3961 ctggcgacgt ggcatggcga ccgcaag
//
LOCUS       DASZAK010000338         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_647976, whole genome shotgun sequence.
ACCESSION   DASZAK010000338 DASZAK010000000
VERSION     DASZAK010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_647976"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..906
                     /locus_tag="VGT44_05960"
     CDS             97..906
                     /locus_tag="VGT44_05960"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV2580380.1"
                     /translation="MLIIGELINCTRKKVGESARARDAEFFRQLARKQVDAGAHVLDV
                     NGGIPDQEVELLAWLVDLIQEVTDVPLSLDSADPEALESALPRCKERPIVNSISDEPA
                     RLALMPLLKEHKPKLIVLSMSEAGPPSGVEDRVAIASKLVDHLTADGFALDDLYVDAC
                     VMPAATSQDQGAKLMAAIHQIGQRYPGIHFSAGVSNVSFGLPQRKLLNEAFILLLMAH
                     GLDAAIIDPCDQQLMANVLAAEALLGRDEYSAEYLRAFRQGRLTAAVPVHV"
     gene            1036..1794
                     /locus_tag="VGT44_05965"
     CDS             1036..1794
                     /locus_tag="VGT44_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580381.1"
                     /translation="MATVSTSHPKWQHAPTGTYDRIFYSGMAVALAATVFTGFAPTFY
                     LRFYFHAPTPSGATSLTRLAQLHGALFSSWVVLFIVQTSLIGKHNVAMHRRLGIAGVL
                     LAAVMTVVGAITGIKAAARGAVPPGADALGFLAIPLGDMLMFSIFVASAFFWRRNREA
                     HKRLMLLAYIGIVAAATARLPGVLPLGPLWFYGLAFIFLLIAVAYDLAFRHRVHIAYI
                     LGGGLLIASVPLRLMFSGTGTWRAIAQFLVGRVG"
     gene            complement(2112..2522)
                     /locus_tag="VGT44_05970"
     CDS             complement(2112..2522)
                     /locus_tag="VGT44_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2580382.1"
                     /translation="MRFNRSVPPCVVIPVLIYPDPGVAADWLSRAFGFTVRLRIANHR
                     IQMKSGEGCFTIAEGNAVPDHSHIVQVRIEDAKGHCERARQNGAIILTEPQDHPYGER
                     QYNAQDFHGHRWDFTETITDVAPEEWSGGAFHLE"
     gene            3022..3540
                     /locus_tag="VGT44_05975"
     CDS             3022..3540
                     /locus_tag="VGT44_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580383.1"
                     /translation="MTRNMVSAILALVAILVALSVAQKSNNATGNFHQLQALAGDWDG
                     KDDAGNTVKTKFELVAGDTAVMETLTMSGMDQMLTLYTVDGNGIALVHYCPTNNQPRM
                     RATPSSAAVKELVFLFQGATNLPDPLIGHEQKLVLQFAGANQIVEHWTWRKDGKDTEM
                     IYNFTRNQAGKR"
BASE COUNT          738 a         1044 c          989 g          798 t
ORIGIN      
        1 aatcgttgca agcgaaccga tgttgcaccg gattctcgaa cggagctatg gagtggatct
       61 cactaagatc gcaatcaaga aaaggatgat gtttctatgt tgataatcgg cgagttaatc
      121 aactgcacgc gaaaaaaggt cggggaaagc gctcgggcgc gagacgcgga gttcttccgc
      181 caactcgctc gcaaacaggt agacgccggg gcgcatgtgc tcgatgtgaa tggcggcatc
      241 cctgatcaag aggtggagct cctggcgtgg ttggtcgact tgatccagga agtaaccgac
      301 gtgccgctca gcctcgacag cgccgatccc gaagctctgg aaagcgcgtt gcccaggtgc
      361 aaagaacgtc caatcgtgaa ttcgatcagc gacgagcctg ctcgcctcgc tctcatgccg
      421 ctcctgaaag agcacaagcc gaagctcatc gtgctttcca tgtcggaggc cgggccgccg
      481 tcaggcgtag aagatcgcgt tgccatcgcc tccaaactgg tcgaccacct cacggccgat
      541 ggttttgccc tcgacgatct ttatgtggat gcgtgcgtca tgccagccgc taccagccag
      601 gatcagggag ccaagctcat ggcggcaatt caccaaatcg gccagcgcta tcctggcatt
      661 catttcagcg ctggcgtcag taacgtctca ttcgggctgc cgcaaagaaa gttgctgaac
      721 gaagcgttca ttctcttgct catggcccac ggccttgacg cggccatcat cgacccctgc
      781 gatcaacaac tgatggccaa cgtgctcgcc gccgaagccc tgctgggtcg cgacgaatac
      841 agcgccgaat atctgcgcgc ctttcgccag gggcgcctga ccgcagctgt accagttcac
      901 gtctgaacgt atggctgcgt gggatattgc cagcgctggg caaccaagct cccatttggt
      961 cccagtggtc tctgcgacag gtgggcctcg taagatcctg atagaatgct cgcggtctcg
     1021 ccggggaggc cgcagatggc aactgtctct acctcgcatc ctaaatggca gcacgcgccc
     1081 actggcacct atgaccgcat cttctacagc ggcatggccg tcgcgttggc agccaccgtc
     1141 tttaccggct tcgctcccac cttttatctc agattctatt ttcatgcgcc tacgccttcg
     1201 ggggcgacat ccctgacgcg gctagcgcag ctacatggtg cactcttttc aagctgggtc
     1261 gtgctgttca ttgtgcagac atccctcata gggaagcaca acgtggcgat gcatcggcgg
     1321 ctggggatcg caggtgtcct gctggcggcg gtgatgacgg ttgtgggtgc aattactgga
     1381 atcaaagcgg cggcgcgggg agcagtcccg cccggagctg atgcgctcgg cttcctcgcc
     1441 attcccctgg gcgacatgct gatgttctct attttcgtcg cgtccgcatt tttctggcgc
     1501 cgcaatcggg aggctcataa acgcctgatg ctgctcgcct acatcggcat tgtcgcggca
     1561 gcaacagcgc gcttgcccgg tgttctgccc ttgggtccac tatggttcta cgggctggct
     1621 ttcatatttt tgctgatcgc ggtggcctac gatctcgcct tccgccaccg cgtacatatc
     1681 gcgtacatct tgggcggtgg tttgctgatc gcatcggtac cgctgcgcct tatgttttcg
     1741 gggacaggga cgtggcgggc gatcgcgcaa ttcctcgtcg gccgagtcgg ctgatgagtg
     1801 aaatcgcgat gttacggcag tcaaccagtc attcggagga ttttacgttg gcgcagagga
     1861 tcgcgacgaa tacagcgccg aatatctgcg cgcctttcac caaggtcgac tggcgccggc
     1921 cgtgccggtt cacgtctgat ccggtctcat gggtttgctt cagccttcat aggaagaccg
     1981 cgttttgatc tcagcgatct ctgcggcagt tctctgcgct ctctgcgttt aaaaagcttc
     2041 ggccggaacg ccatccgagg atctcccctc aacgcgaccg gccaccgcct ctcacctttc
     2101 acaacgcaga ctcattcaag atgaaatgcg ccaccgctcc attcctcggg ggcaacatcg
     2161 gtgatggttt ccgtgaagtc ccaccggtgg ccgtgaaaat cttgcgcatt gtattgccgt
     2221 tcgccatagg gatggtcctg cggctcagtc aggatgatgg caccattctg ccgcgcgcgc
     2281 tcgcagtgac ccttagcatc ttcgatgcga acctgtacga tgtgcgaatg gtcgggcacg
     2341 gcgttgcctt ccgcgatggt gaaacaaccc tccccagatt tcatttgaat acgatgattg
     2401 gcgatgcgca gacggacagt gaagccaaac gcccgcgaca gccaatcggc ggcaaccccc
     2461 gggtcggggt aaatgagaac gggaatcaca acgcacggcg ggaccgatcg gttgaatcgc
     2521 atctggaaac tttatcactc acctcgagca acagttccac cggtcggcct acctggctgt
     2581 tttccgacgg cgtgcttcga ctcgctgctt cgcctcttcc aaacggcaag caccatctgc
     2641 cttcagctcc tcgatgctcg atttgcagcc atgttctcgc tcgtgatgat gcattttctc
     2701 gaaactcgcg cgggccgcag cgttcaactc tttgttcctt tgtgctccac ttggacgccg
     2761 cgtgttggct ctctcaatcc ttctggcaag tatcaggaat tctgtatgta gcttgttcca
     2821 gagcggacaa atcatgagga catattggca tttgggagag aacagacaaa acgactaccg
     2881 ttggttcata aatgcgaaaa atgcagcgaa ctgccgccgt cttgcagtca ccggtctggc
     2941 tccggcagca aaaatacagg atactcggca actgcccaac caggcagctc gtgacaatct
     3001 ctggtaaatg atggaggctc gatgacccga aatatggttt ccgccattct ggcattggtt
     3061 gccattctgg tcgccctgtc ggtggctcaa aagtccaaca atgcaaccgg caattttcat
     3121 caacttcaag ctctggcagg agactgggat ggaaaggacg atgccggcaa taccgttaag
     3181 accaagttcg aactggtcgc gggcgatacc gccgtcatgg agaccctcac aatgtcaggg
     3241 atggaccaaa tgttgaccct ttataccgtc gacggtaatg gaatcgcgct ggttcactac
     3301 tgcccgacca ataatcagcc acgcatgcgg gccacaccat cttcggcggc ggtcaaagaa
     3361 ctggtgtttt tattccaagg agctaccaac cttcccgatc cgttgattgg ccacgaacaa
     3421 aagctggtcc tgcagtttgc aggtgcaaat caaattgtcg agcattggac ctggcgaaag
     3481 gatggcaagg ataccgaaat gatctacaac ttcacccgca atcaggccgg aaaacggtaa
     3541 tcagcacgat gcgatgcgtc aggacttct
//
LOCUS       DASZAK010000339         1767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_79867, whole genome shotgun sequence.
ACCESSION   DASZAK010000339 DASZAK010000000
VERSION     DASZAK010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79867"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VGT44_05980"
     CDS             complement(<1..872)
                     /locus_tag="VGT44_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580384.1"
                     /translation="MHETIASKQANGNRETEETFAPRITYHRVTLGALTREGHALAEI
                     VEGDAQDGQARRALAVPGGLPGEEVTIAIETFANKPPKRHRRRWKARPPRVSISEIHR
                     PSPLRVPARCPVFGECGGCQLQHMDYAAQLGWKRQVVADLLREIGRFDDPPVLEPVAC
                     ANPWNYRNHMRFSVNRDGQPGLTARGSHRVLSLETCPIAHEQINQALRVLSSVPTARP
                     QLLIRCGAATGQVLIQPAPNAVVAQQIAEAGLDLRAETMEEQLAGVTFHIRPSSFFQT
                     NTEQAEQMMRMVLQG"
     gene            complement(885..>1767)
                     /locus_tag="VGT44_05985"
     CDS             complement(885..>1767)
                     /locus_tag="VGT44_05985"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2580385.1"
                     /translation="IVWRTLYMLHYLDALLVKNPLDSRLYSDAVGACFTLGLSERCLV
                     YLSRWAEHFPHDPQYIGILQQLGSTEALLAADYRTLGLKQRANRFFELAEQDLLKAAS
                     VKPSPTNYMLLGEMKLGQGDVDAAIAYLQQGLAADPTPREEAQVEYDLANIDMQLKKP
                     EEALRHFQRVEMLDPSIKNLWIRMGQAHRELKDVASAERYLTRALEEDPQELAPYVSL
                     AALYAGNNDLARSRKLVDEGLRLHPDSVHLRALLASLLYDQGETRRAEAELRLAEQLD
                     PDYPLVKALRDVMSKAR"
BASE COUNT          307 a          544 c          549 g          367 t
ORIGIN      
        1 ccttgtagaa ccatacgcat catctgctcg gcctgctccg tatttgtctg gaagaacgag
       61 cttgggcgaa tgtggaaggt gactccggcc aactgctctt ccatcgtctc cgcgcgcagg
      121 tctagaccgg cctcggcgat ctgctgcgcg acgactgcat tgggcgctgg ctggatcagc
      181 acctggccgg tcgctgcccc gcagcggatc aagagctgtg gccgcgcggt tgggacgctg
      241 ctcagcacgc gcagggcttg gttgatctgt tcgtgcgcga tgggacacgt ctccagcgac
      301 agcacgcgat gactgcccct ggcggtcagg ccaggctgtc catcgcgatt gacggagaag
      361 cgcatgtggt tgcgatagtt ccagggattg gcgcaagcaa ccggctcaag cacgggcggg
      421 tcatcgaagc ggccaatctc cctgagcaaa tccgccacga cctgtcgttt ccagcccagc
      481 tgcgcggcgt agtccatgtg ctggagctgg cagccgccgc attcgccgaa gacgggacag
      541 cgcgccggga cgcgcagggg cgatggccgg tgtatctcgc tgatcgagac acgcggcggg
      601 cgcgctttcc agcggcgtcg atgtcgtttg gggggcttat tcgcaaaggt ttcgatggca
      661 atcgtgacct cttcccccgg caaacccccc ggaaccgcca gcgcgcgcct ggcctggccg
      721 tcctgcgcgt cgccctcgac aatctcggcc agcgcgtgtc cctcgcgcgt cagcgcgccg
      781 agcgtgacgc gatgatacgt gatgcgcggc gcaaacgtct cttccgtctc tctgttccca
      841 ttcgcttgct tgcttgcaat ggtttcatgc actgctttac tgcctcatcg cgctttactc
      901 attacatcgc gcagcgcctt cacgagcggg tagtcgggat cgagctgctc ggccaatctc
      961 agttcagcct ccgctcgcct ggtttccccc tgatcgtaaa gcaggctggc caatagggcg
     1021 cgcaaatgca cggaatcggg atgcaaacgc aggccctcat cgacaagctt gcgggatctc
     1081 gccaggtcat tgttgccagc atacagcgcg gccagtgaaa catacggagc gagttcttgt
     1141 gggtcttcct ccagcgcgcg cgtcaggtat cgctcagccg acgccacatc tttgagctcg
     1201 cggtgagcct ggcccatgcg gatccagaga ttcttgatac tgggatcgag catctcgact
     1261 cgttggaaat ggcgcagggc ttcttccggc tttttgagct gcatatcgat attcgccagg
     1321 tcgtattcga cttgcgcctc ttcgcgcggc gtcgggtcgg ctgccaatcc ctgctgaagg
     1381 taggctatgg cggcatccac atctccctgc ccgagcttca tttcgcccag cagcatatag
     1441 ttggttggcg aaggcttgac gctcgcggct ttgagcaaat cttgttcggc aagctcaaag
     1501 aaacggttgg cgcgttgttt caggccgagt gtccggtagt cggcagcaag cagtgcctcc
     1561 gtcgagccaa gctgctgtag gatacctatg tactgcgggt catgcgggaa atgctccgcc
     1621 cacctgctga ggtacaccag gcagcgctcg ctcaatccga gggtgaagca ggcaccgacg
     1681 gcatccgagt acaggcgtga gtcgagggga ttcttgacca gcagcgcatc caggtaatgc
     1741 agcatgtaga gggtgcgcca gacgatc
//
LOCUS       DASZAK010000340         2818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_184939, whole genome shotgun sequence.
ACCESSION   DASZAK010000340 DASZAK010000000
VERSION     DASZAK010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184939"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VGT44_05990"
     CDS             <1..522
                     /locus_tag="VGT44_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEV2580386.1"
                     /translation="SDIAIGLRLPFAVAEELKVTYGHCDPSTIADDDMIDLSHYLPDC
                     NDLVPRKLLAEIVQARVEELFEMVHEEIKKSGFDGLLPAGLVVTGGGAELPGILDTAG
                     QILDLPARLGSPLGLYGLADSISRPAYATSVGLLLWGLRHTSFLTSEEEQEEMGTGDM
                     ISRLGRWLRAFVP"
     gene            640..2103
                     /gene="ftsZ"
                     /locus_tag="VGT44_05995"
     CDS             640..2103
                     /gene="ftsZ"
                     /locus_tag="VGT44_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV2580387.1"
                     /translation="MGNHQVEGFAQIRVIGVGGGGSNAVNRMIQANMMGIEFIAVNTD
                     AQALLLTDTPHRIRIGDKLTRGLGAGGNPNVGAKAAEENTEDLYEALKGSDMVFITAG
                     MGGGTGTGASPIVAQIARELGALTVGVVTKPFAFEGNKRRIAAEEGIANLKQHVDTLI
                     TVPNDRLLQVADKKMPLKEAFRLADDVLRQGIQGISDLITVPGLINLDFADVKTIMSA
                     AGSALMAIGEANGETRAIDAARMAIASPLLDIDINGARGVLFNITGGLDLTLYEVNEA
                     ADIISTAAHPEANIIFGAVQDPAYDGKVKITVIATGFDGQTHLSTSPQRVEYNRSMFY
                     QVAAPSSNGNAAMRPQQQSVPSGYSSMPVAPRAPMNIAKHPTGPLQPPGRIMQPTPVP
                     VMQEQMRQPAGVRRAEVREDDFDLDNDSGDVLETYNERRRLPVPEPEAQPSQRPAADS
                     RQQRPIRRMDPKALDLPRGNRTPPGDVIDIPAFLRKR"
     gene            complement(2149..2568)
                     /locus_tag="VGT44_06000"
     CDS             complement(2149..2568)
                     /locus_tag="VGT44_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV2580388.1"
                     /translation="MTGQNDYNRQLIEEFRADRSKSGGSFASRPLLLLTTTGAKSGQR
                     RTTPMMYVPDGGRLLVIASNAGAPAHPDWYYNLVAHPEVTVEVGNETYDATAVVTQGA
                     ERDRLWTSIVDKYPFFTEHQAKITRQIPVIALSRRKQ"
BASE COUNT          663 a          711 c          843 g          601 t
ORIGIN      
        1 agcgatatcg cgatcggctt gcgcctgcca ttcgctgtag cagaggagct gaaggtgacc
       61 tacggtcatt gtgacccatc aactatcgcg gatgacgaca tgattgacct gtcgcactac
      121 ctaccagatt gtaacgatct tgtgcctcgg aagctacttg ccgaaatcgt ccaggcgcgt
      181 gtggaagagc tatttgagat ggtacacgag gagatcaaga aatccggttt tgatgggtta
      241 ttgccagcgg ggctggtagt aacaggaggt ggcgctgaac tgccagggat tctagacacc
      301 gcggggcaga ttcttgattt gccggcgcgt ctggggtcgc cacttggttt gtatggactc
      361 gccgattcca tcagtcgtcc tgcctatgcc acctcggttg gtctattgtt gtggggtttg
      421 cgccacacct catttcttac aagtgaggaa gagcaggagg agatggggac aggggatatg
      481 atatctcgtt taggacgctg gttacgcgcg ttcgttccat agaattgatt gtagggggcg
      541 aggaattgct cccgccagga ttgccatcgc ctgtttgtct gtcgggtacg gtcgcctgta
      601 ctggaacagc aaacacgaaa gggggccaaa atgctaccaa tgggaaatca tcaagttgag
      661 ggcttcgccc aaatccgtgt catcggtgta ggaggagggg gcagcaacgc agtcaaccgc
      721 atgattcagg ccaacatgat gggcattgag ttcatcgccg tgaataccga tgcccaggcc
      781 ttgctgctga cagatacgcc acaccgtatc cgcatcggtg acaagctcac gcgcggcctg
      841 ggcgccggtg gcaacccaaa cgtcggagca aaggctgcgg aggagaacac cgaagacctg
      901 tatgaggcac tgaaaggctc cgatatggtc ttcatcacag cgggaatggg aggtgggact
      961 ggtacggggg caagcccaat cgtggcgcag attgcgcgcg agttaggggc tttgaccgtc
     1021 ggcgtggtca cgaagccatt cgccttcgag gggaacaagc gccgtattgc ggctgaggag
     1081 ggcatcgcca atctcaagca acacgtcgat acgctgatca ccgtacccaa cgaccgactg
     1141 ttgcaggtgg ctgacaagaa aatgccgttg aaagaggctt tccgcctggc agatgacgtg
     1201 ctgcgccagg gcatccaggg cattagtgat ttgatcacag taccagggct gatcaacctt
     1261 gacttcgctg acgtgaaaac tatcatgtcc gctgccggtt ccgcattgat ggcaattggc
     1321 gaggcaaacg gcgagactcg tgccatagat gcagcgcgga tggcgattgc cagtccctta
     1381 ctggatatcg atatcaatgg tgcgcgcggc gtactgttca atatcaccgg tggccttgac
     1441 ctgacattgt acgaagtgaa tgaagcggct gatatcatca gcacggcagc gcatcctgaa
     1501 gcgaatatca tctttggcgc agtgcaggac cctgcttatg atgggaaagt caagatcaca
     1561 gtgattgcga cagggtttga tgggcaaaca cacctgtcaa catcaccaca acgtgtggag
     1621 tacaatcgaa gcatgttcta ccaggttgcg gcaccgtcca gcaatggcaa cgccgcaatg
     1681 cgcccgcaac agcaaagcgt tccaagtggg tacagctcga tgccggttgc accaagggca
     1741 ccaatgaaca tcgcgaagca tccaacgggg ccattacagc ctccggggag gattatgcag
     1801 ccaacgccgg tgcctgtcat gcaggaacag atgaggcagc ccgcaggtgt aaggcgcgct
     1861 gaggtgcgag aggacgattt tgacctggat aacgattctg gcgatgtgct tgagacctat
     1921 aacgagcgca ggcggcttcc tgtacccgag ccagaggcac agccatcaca gcgcccggcg
     1981 gctgattcac gccaacagcg cccaatccga cgcatggacc cgaaggcgct cgatctacca
     2041 aggggcaatc ggactccccc gggtgacgta atcgatattc cggcgtttct gcggaagcgg
     2101 taagtaggga acagggagag tattagaaga gggccacagt ggccctcttc actgttttcg
     2161 tctagaaagc gcgatcacgg ggatctggcg ggtgattttc gcctggtgtt cggtaaaaaa
     2221 tgggtacttg tccacgatgc tggtccaaag ccgatctcgc tctgcacctt gcgtaaccac
     2281 agcagtggca tcgtaggtct cgtttccgac ttcgacggtg acttccgggt gagctaccag
     2341 attgtagtac caatcaggat gggccggggc gccggcgttg gaagcaatta cgagcaggcg
     2401 gccaccatca gggacgtaca tcattggcgt tgtgcggcgc tggccgcttt tggcacccgt
     2461 ggttgtgagc aggagtagcg gcctgctcgc gaatgatcca ccgctcttgc tgcggtccgc
     2521 gcggaattcc tcaataagct gacggttgta gtcgttttga cctgtcatga tttctcctca
     2581 tttttatcac gcataagcgc aggcatttct tcttcgattt agacatagta acaagcacag
     2641 agcattctag cacagaaaca acaagcgata ttgtaaaaac gcgacaggat gaatgtttgt
     2701 gctataaata acgtagaaaa tgagtcgtgt catccgtagc gttcgggatg atgaatccca
     2761 aagttaaagg ctggatttgt ggtggaaagc atgatgaagc caaccatcgg aaaaccca
//
LOCUS       DASZAK010000341         5238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_221621, whole genome shotgun sequence.
ACCESSION   DASZAK010000341 DASZAK010000000
VERSION     DASZAK010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221621"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1023
                     /locus_tag="VGT44_06005"
     CDS             <1..1023
                     /locus_tag="VGT44_06005"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2580389.1"
                     /translation="FSRLLRFEYGSDTFYSNMVRLSLKRWKNLEGVAGRTLYTPTGLL
                     VLGNEHDMFTQSSYQVVRALGLPSELLSKEDCELRFPQFDTQHFDIRTYNPDAGILHA
                     STCLQTLRDLVLYNGGEIYESCRVTKLISDSQFHPVQLRLSTGTEISADRVVLATGSW
                     IHRLLSDLYIPVRMTRQYMLYFAGLHCSLYSAGAFPAFFAGDLYGLPIHQGCNGWVKA
                     TSHELGVPTDPDKVTPPDDQVVAGIARKLRELLPALGHAEIARIDSCMYDVTPDENFI
                     LDRLPYDPRVIFASGLSGHGFKFGLLLGELLCSMVCDTHPPVPLERFRLSRFAHQQER
                     QRTSVA"
     gene            complement(1107..2513)
                     /gene="leuC"
                     /locus_tag="VGT44_06010"
     CDS             complement(1107..2513)
                     /gene="leuC"
                     /locus_tag="VGT44_06010"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEV2580390.1"
                     /translation="MPRTMFEKIWDAHVVRDASEESTILYIDRHLVHEVTSPQAFTGL
                     RLTGRAVHRPDATIAVLDHNIPTVPGRRMLDVVDAECVESIRTWEQNAQTYGINLFHM
                     SDARQGIVHVIGPEQGLTLPGMTIVCGDSHTSTHGALGALAFGIGTSEVEHVLATQCL
                     LQERPRTMNIQVEGTLAPGVTAKDLALAIIGQLGTGVGTGHVIEFSGSTIRSLSMEGR
                     MTLCNMAIEAGARAGMVAPDETTFAYIKGRTYAPQGEMFEQAVTAWRGLASDPGARFD
                     SVQQVNIEGLGPQVTWGINPGMVSDITSVVPDPAEQSDPEKRKAYARALTYMGLTPGQ
                     KITEIPVDTVFIGSCTNSRLEDLRMAADYVKGRHVAPHVRAMVVPGSTQIRRRAEEEG
                     LADIFREAGFQWREAGCSMCIAMNGDQLSEGERCASTSNRNFEGRQGRGGRTHLVSPA
                     MAAAAAIAGHFVDIREML"
     gene            complement(2528..4201)
                     /gene="ilvD"
                     /locus_tag="VGT44_06015"
     CDS             complement(2528..4201)
                     /gene="ilvD"
                     /locus_tag="VGT44_06015"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV2580391.1"
                     /translation="MRSDQIKVGFERAPHRGLLRATGVVGENDFNKPFIAVCNSYVDV
                     VPGHVHLQAFGKLVKEAIRAAGGVPFEFNTIGVDDGIAMGHIGMKYSLPSRELIADCV
                     ETMVEAHRFDGMVCIPNCDKIVPGMLMAAMRVDIPTIFVSGGPMAAGKLADGRTVDLI
                     SVFEGVGAYQAGKIDDRELLELEQQACPTCGSCSGMFTANSMNCLMEALGMALPGNGS
                     YLAMSEARRELVREAGRMIMRLIEANLTPRQIVNAESIDNAFALDMAMGGSTNTVLHT
                     LALAREAGVEYSLERINQVAARIPHICKVSPAGKWHMEDVDRAGGIGAILKELSRKPG
                     ALNLDRPTVTLRTLGENIANAEIKDPEVILSIDAPHSERGGLALLFGNLAPDGAVVKV
                     GAVAPSMMRHCGPARIYNSQEEACAGILNGRVQAGDVVVIRYEGPRGGPGMQEMLAPT
                     ANIVGMGLGEKVALITDGRFSGGTRGACIGHVSPEAAAGGPIAALVDGDMINIDLVEN
                     RLDVQLSPEQIAQRLEKVTPPKERIKSRWLRRYASLVTSANTGAVLAEV"
     gene            complement(4216..5214)
                     /locus_tag="VGT44_06020"
     CDS             complement(4216..5214)
                     /locus_tag="VGT44_06020"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEV2580392.1"
                     /translation="MTQTEATEMDQSSQPEKAESPKIPVLSPDEGYAYFEGRIIPMSE
                     AKVSIATHALQYGTACFEGIRGYWNEQQQQLYLLKLREHFQRMADSWNVIRMKPKESI
                     DDLCRITVELVRQHGYHQDVYVRPLAYKASRTIKLTLSSLDDAVAIYTFPMGNYLDIS
                     AGLSVCTSSWRRANSNAMPVRAKVTGAYINSSLAVDDATAAGFDEAILLTHDGTVSEA
                     SSCNIFLLRNGKLSTPALSEDILEGITRNALIDMVKDEFDIKVEERRIDRTELYSADE
                     IFMCGTGVQVSPVTSVDHRPVRTGKPGPMTMKLQELYLSACRGENEKYKDWVTPVF"
BASE COUNT         1173 a         1593 c         1248 g         1224 t
ORIGIN      
        1 ttttcacgcc tactacgctt cgagtatgga tctgacacat tctactcaaa tatggtccgt
       61 ttaagcctca aacgttggaa aaatcttgaa ggagtagcgg gacgcactct ctacactcca
      121 acaggcctgc ttgtgctggg aaacgagcat gatatgttca cacagtcaag ttaccaggtt
      181 gtacgagcat tagggctacc ctcagaactg ctatctaaag aggattgtga gctgcgtttt
      241 ccccagtttg atacgcagca tttcgacata cgtacctaca atcctgatgc aggcattcta
      301 catgcttcaa cctgcttgca gacgctcaga gacctggttc tctataatgg aggagaaatt
      361 tacgaatcct gtcgcgtcac caaacttatc agtgacagcc agttccatcc ggtccagctt
      421 cgtttgagta ctggaacgga gatttctgca gaccgcgttg tgctcgcaac cggatcctgg
      481 attcatcgct tgctttctga tctgtatatt ccagtacgaa tgacgcgcca gtacatgctc
      541 tatttcgccg gtctgcattg ctcattatat agcgcaggcg catttcccgc tttttttgct
      601 ggagacctct acggccttcc tatccatcaa ggttgcaacg gttgggtaaa agctacctcg
      661 cacgaattgg gcgtccctac tgaccctgat aaggtcactc ctccagacga tcaagtggtt
      721 gccggcatcg ccaggaaact gcgagaactc ctaccagcac tcggccacgc ggagatagca
      781 cgcatagatt catgtatgta cgacgtcaca ccggacgaaa atttcatcct cgaccggctt
      841 ccatacgatc cccgcgtcat ctttgcctcc ggcctgtcag gacacggctt caagttcggc
      901 ctgctcctgg gagagctatt atgtagtatg gtctgtgaca cccacccacc cgttccgcta
      961 gagcgcttcc gcctttcgcg tttcgcccac caacaagaac ggcaaaggac ctccgttgca
     1021 tgatgcaggg gtggggacgc ttgtgtcccc acccctgcat catgcaacca tacgagatcc
     1081 ttcgcatcgc tcaggatgac agtgcgctat agcatttcgc gtatatccac aaagtgcccg
     1141 gctatagcgg cagcagcggc catcgcggga ctaaccaggt gtgtccgtcc accgcgtccc
     1201 tgccgtccct caaagttgcg gttgctcgtc gaggcacaac gctccccttc ggacagttgg
     1261 tctccgttca tagcgatgca catgctgcat cctgcttcgc gccactggaa gcccgcctca
     1321 cggaagatat cggccagtcc ttcctcctcg gctctccggc gtatttgcgt tgaccctggc
     1381 acgaccatcg ctcgcacgtg cggtgcgacg tgcctccctt tgacgtagtc cgctgccatg
     1441 cgcaggtctt ccagccgtga gttggtgcag gagccaatga acaccgtatc cacaggaatt
     1501 tcagtgatct tctgcccagg cgtcaggccc atataggtta gtgcgcgtgc atatgcttta
     1561 cgcttctcgg gatcggactg ctcggcaggg tcaggcacaa cactggtaat atcagacacc
     1621 atgcctggat tgatgcccca ggtcacctgt ggtcccagtc cctcgatgtt cacttgctgc
     1681 actgagtcaa accgggcacc gggatcactg gcaagccctc gccaggctgt gaccgcctgc
     1741 tcaaacatct cgccttgagg cgcgtacgtc cgtcccttga tataagcaaa ggtcgtttca
     1801 tcgggagcca ccatccccgc acgcgctccc gcttcaatcg ccatgttaca cagggtcatg
     1861 cgtccctcca tcgacaggct gcgaatggtc gaaccgctaa actcgatgac atgtcctgtg
     1921 ccgacgcccg taccaagttg acctatgatc gctaacgcca ggtctttggc tgtcactccc
     1981 ggagccaggg taccctctac ctggatattc atcgtcctgg gccgctcttg taacaggcac
     2041 tgggtagcca gcacatgctc gacctcgctg gtgccaatcc caaaagccag tgcgcccagc
     2101 gcgccatgcg tcgaggtatg tgaatcccca cacacaatcg tcatccccgg caacgtcaac
     2161 ccttgttcgg gtccaatcac atgcacaatc ccctggcgag catcagacat atgaaagaga
     2221 ttaataccat acgtctgtgc gttttgttcc catgtcctta tagactcgac acactcggca
     2281 tccaccacat ccaacatacg ccttccagga acagttggaa tattatgatc aagcaccgcg
     2341 atggttgcat caggacgatg tactgcgcgg ccggtgagtc gcaatccggt aaacgcctgc
     2401 ggggacgtca cctcgtgtac aaggtgtcga tcgatatata aaatggtgga ttcttcagaa
     2461 gcatcacgta ccacgtgagc atcccagatc ttctcaaaca tagtccttgg catacataaa
     2521 ctccatctta aacctcagcc agcacagctc ccgtatttgc tgaagtcacc agcgatgcat
     2581 aacgacgcag ccagcggctc ttaatgcgtt ccttcggcgg agtcaccttc tcaaggcgct
     2641 gggcaatctg ctcagggctc aattgcacgt cgagccgatt ttctacaagg tcgatattga
     2701 tcatatcgcc atcgacgagc gccgcaatcg gtccgcctgc cgccgcctct ggagatacat
     2761 ggccgataca tgctccacgc gtgccaccgg agaaccgccc gtctgtgatc agcgcgacct
     2821 tctcgccaag tcccataccc acgatattgg ccgtcggcgc cagcatctcc tgcataccgg
     2881 gaccgcctcg cggcccttcg taccggatca cgacaacatc acccgcttgt acccgcccat
     2941 tcagaatacc cgcgcaggcc tcttcctgcg agttgtagat gcgtgccggt ccgcaatgtc
     3001 gcatcatcga gggagctacc gctccaacct tcacaactgc gccgtcagga gcaagattgc
     3061 cgaagagtaa agccagccct ccgcgctcgc tgtgtggcgc atcaatggaa aggataactt
     3121 ccgggtcttt aatctccgcg ttggcaatat tttcgcccag ggttcttagc gtcacagtgg
     3181 gacgatcaag attgagcgcg ccaggcttcc tggaaagttc tttcaaaata gcgccgatac
     3241 cgccggcccg atcaacgtct tccatatgcc atttcccggc cggactgact ttgcagatgt
     3301 gcggaatgcg cgctgccacc tggttgatac gttccaggga atactctacc ccggcttctc
     3361 gtgccaatgc caacgtatgc agcaccgtgt tggtcgagcc acccatagcc atgtccaatg
     3421 caaaagcatt gtcgatggac tcagcattga cgatctggcg cggtgtgaga ttggcttcaa
     3481 tcaagcgcat gatcatgcga cctgcttcac gaacaagctc tcgacgcgcc tcactcattg
     3541 ccaggtacga gccatttcca ggcagagcca tacccaacgc ttccatcagg cagttcatcg
     3601 aattggcggt aaacatgccc gaacaagaac cacacgttgg gcaagcttgc tgttccaatt
     3661 ccaatagttc gcggtcgtcg atcttgcctg cttgataagc gccaacacct tcgaagacac
     3721 tgatcaagtc aacagtccga ccatccgcca gcttacccgc ggccatcggc ccaccactga
     3781 cgaagatcgt gggaatatca actcgcatag ctgccatcag catacccggc acgatcttgt
     3841 cacagttagg tatgcacacc atcccatcga agcggtgagc ctcgaccatc gtctcaacac
     3901 agtcagcaat caactctcga ctgggcagcg aatatttcat accgatatgg cccatagcga
     3961 tgccgtcatc cacaccaatc gtgttgaatt caaaaggcac gccgcctgct gctcgtatcg
     4021 cctccttgac cagcttacca aaagcctgta agtgtacatg ccccggcacc acgtcaacat
     4081 acgaattgca cacggcgata aacggcttat taaaatcatt ttctcccacc acacccgttg
     4141 cgcgcagcag cccgcggtgt ggggcccgct caaagcctac ttttatttga tctgaacgca
     4201 tgacaattcc tcatcttaaa acaccggtgt tacccagtct ttgtattttt cattctcgcc
     4261 acgacaggcg gacagataga gttcttgcag tttcatcgtc attgggcccg gttttccagt
     4321 tctcaccggg cgatgatcaa cgctggttac aggtgaaacc tgcacccctg taccgcacat
     4381 gaagatttcg tctgcggaat agagctcggt gcggtcaatg cgccgctcct cgacctttat
     4441 atcgaattca tctttcacca tatcaatcag cgcattgcgg gtgattccct cgagaatatc
     4501 ctccgaaagc gcaggtgtgc tgagtttccc attgcgcaac aaaaagatgt tgcaagaact
     4561 cgcctccgaa acggtgccgt catgggtgag caagattgct tcgtcaaaac cggcggcagt
     4621 ggcatcatcc acggccaggg aactattgat gtaagcccca gtaaccttgg ctcgcaccgg
     4681 catcgcatta ctgttggcac gcctccagga cgaggtgcag acgctcaaac ccgcgctgat
     4741 gtcgaggtag tttcccattg ggaacgtata gatagcgact gcatcatcca atgaactgag
     4801 cgtgagttta atggtgcgag atgccttgta tgccagggga cgcacgtaga catcctggtg
     4861 ataaccatgc tgcctcacaa gctcgaccgt gatacgacac aagtcgtcaa tcgattcctt
     4921 aggcttcata cgtataacat tccacgaatc ggccatacgc tgaaaatgtt cgcgcaattt
     4981 aagcaggtac aattgctgtt gttgctcgtt ccagtagcca cggatgcctt caaagcaagc
     5041 tgtaccatat tgcaacgcgt gggtcgcaat gcttactttg gcctcgctca tcggtatgat
     5101 tcgcccctcg aaataggcat agccttcgtc agggctgagt accggaatct ttggcgattc
     5161 tgccttctca ggttgactac tttggtccat ttctgtggct tcagtctgag tcacacaatt
     5221 cctccttata tttccatc
//
LOCUS       DASZAK010000342         2276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_328311, whole genome shotgun sequence.
ACCESSION   DASZAK010000342 DASZAK010000000
VERSION     DASZAK010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328311"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..957)
                     /locus_tag="VGT44_06025"
     CDS             complement(<1..957)
                     /locus_tag="VGT44_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580393.1"
                     /translation="MEIQVEQALTSKQIALSDPGEVVDYYYRQGWTDGLPVVPPTPAK
                     VREFLEYSGNFPGDILGVIPARNRAITAEKVAINAVMAGCLPNYMPVIVAAIEAMCQE
                     PFNLHGITATTGGTAPLLIINGSVAKQLNINGGVNCFGPGVRANATIGRAIRLILMNV
                     CGSVPGVLDKACLGHPGKYSYCIAEDEDGNPWGPLSIERGIPQGISAVTVFAGEAPHF
                     VNSQAGGTGKRLVLSIVNTMMGSVYMGGNWVLVLCPEHVTIFKQEGWSKADIREAVYE
                     QAARPLAQFKRIAGLPDSKITPEDEQTLYHNVHTPDDLLIVTA"
     gene            1124..>2276
                     /locus_tag="VGT44_06030"
     CDS             1124..>2276
                     /locus_tag="VGT44_06030"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2580394.1"
                     /translation="MAELEGRTLGRYELRRLIGKGGMANVYEAFDTLSGRVIAVKIFK
                     REDDELLSRFLREAQLMSALHGHHLVPIYFFGTEVLDNALLYYIAMPFLPGGTLRARI
                     RRGPLTLDETCRALSEIADALDYVHSQGIIHRDIKASNVLIDSDGGYYLADFGIARIT
                     SDLTHMTTTGNVLGTVDYVAPELFEVDRKANAFSDLYSLGVLLYEMVTGRLPFIAENQ
                     IALVTMHMRQRPPAPRGIDPRIPAAVERVMLKALEKRPEQRYHSAAELARAFCLAVNA
                     DDVMLISPASATPGLLAPVFKQEQLVLSPHDVGTADFISPIYEPAELVAASSLAPGRD
                     VPAAPSYVPVSAATSRAARRARPRDTRPFYRHPLFVLVVVLLAFTIIAGS"
BASE COUNT          438 a          654 c          667 g          517 t
ORIGIN      
        1 tgccgttacg atcagcaggt catcaggggt atgaacgttg tgataaagcg tttgctcgtc
       61 ttccggcgtg attttactgt ccggcaagcc tgcgatgcgt ttgaattgcg ctaaggggcg
      121 cgcggcctgc tcgtagacgg cctcgcggat gtcggccttt gaccagccct cttgtttgaa
      181 gatggtgacg tgttccgggc aaagcaccag tacccagttg ccgcccatgt agacgctccc
      241 catcatggta tttacgattg acagcaccag gcgtttgccg gtgccccctg cctggctgtt
      301 gacgaaatgg ggagcttccc cggcgaagac ggtcacggcg ctgatgcctt gcgggatgcc
      361 gcgttcgatg ctgagcggcc cccagggatt gccgtcctcg tcttccgcga tgcagtagct
      421 atatttgccg ggatgcccaa gacatgcttt gtcgagtacg ccggggacgg agccgcatac
      481 attcatgaga atcaaacgga tggcgcgccc aatcgtggcg ttggcgcgca ctcccggccc
      541 gaagcagttc acgccgccat tgatgttgag ctgtttcgcc accgagccgt tgatgatgag
      601 cagaggcgcg gtgcctccgg tggtggccgt aatgccgtgc aggttgaacg gctcctggca
      661 catggcctcg attgcggcca cgatgaccgg catgtagttg gggagacagc ccgccatgac
      721 cgcgttgatg gccaccttct cggcagtgat agcgcggttg cgcgccggaa tgacgcccag
      781 gatatcgccg gggaaattgc cggaatactc aagaaattcg cgaactttgg cgggcgtggg
      841 aggcacgacg ggtaaaccgt ccgtccagcc ctgccggtag taatagtcga cgacctcgcc
      901 aggatcggat aatgctattt gtttggatgt aagcgcctgt tccacctgaa tttccatgca
      961 gtgtctcctt agctgagggc gaatgcttgc ctgtgaatca tagcatatca caccctcccg
     1021 actacagggg agggcagtcg taacgaattc tgcccgaatc cgtttaatat ctatgtggca
     1081 taatcaatta agaacttatc aatataatgg aagaggatat tgtatggccg aactggaggg
     1141 cagaaccctc ggtcgctatg aattgcgtcg tctgatcggc aagggtggga tggcgaatgt
     1201 ctacgaggcg ttcgatacgt tgagtggacg ggtgatagcc gtcaagatct ttaagcgcga
     1261 ggatgacgaa ttgttgagcc ggttcctgcg cgaggcgcag ctcatgtccg cgctgcatgg
     1321 ccatcacctc gtgcctatct atttcttcgg caccgaagtt ctcgataacg ccctgctcta
     1381 ctacatcgca atgccctttc tgccgggagg cacactgcgc gcgcgcatcc gtcgcggacc
     1441 gctgacgctc gacgagacct gccgcgcgct gagcgagatt gccgacgcgc tggattatgt
     1501 tcacagccag ggcattattc atcgcgacat caaagcctcg aatgtattga ttgatagcga
     1561 cggcggctat tacctggccg attttggtat tgcgcgcatc accagcgatc tgactcacat
     1621 gactaccacc ggcaacgtgc ttggcacggt cgattatgtc gcgcctgagt tgtttgaggt
     1681 tgatcgcaaa gccaacgcct ttagcgatct ctactcgctg ggcgtcttgc tttacgagat
     1741 ggtcaccggc cgcctgccct ttatcgccga gaaccagatc gcgctggtga cgatgcatat
     1801 gcgccagcgc ccgcccgcgc cgcgcggcat cgatccccgc attcctgcag cggtcgaacg
     1861 tgtgatgctc aaggcgttgg agaagagacc ggagcagcgc taccacagcg cggcggaact
     1921 ggccagggca ttttgccttg cggtcaacgc ggacgatgtc atgctgattt ctccagcttc
     1981 agccactcct gggctgctcg ctccagtatt caaacaagag cagctggttt tatcgccgca
     2041 tgatgtagga acagccgact ttatctctcc aatctatgaa cctgcagaac tggttgcagc
     2101 ctcatcactt gctcctggcc gggacgtacc tgccgcgcct tcgtatgtgc ctgtgtccgc
     2161 tgcgacatcg cgagcagcga gaagagcgcg ccctcgcgat acacggccat tctatcgcca
     2221 ccctttattt gtgctggtcg ttgtgctgct ggccttcacc atcatcgcag gctccc
//
LOCUS       DASZAK010000343         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_291966, whole genome shotgun sequence.
ACCESSION   DASZAK010000343 DASZAK010000000
VERSION     DASZAK010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291966"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1409)
                     /locus_tag="VGT44_06035"
     CDS             complement(<1..1409)
                     /locus_tag="VGT44_06035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2580395.1"
                     /translation="MLQNAQNNANQDRHMFAKKMSERESQRAYRQAIRELVSRFHGEI
                     LFPGDPDYESARVVWNGVGNRHPALIARPAGVEDVISSVNFAREQGVEVAVRSGGHSM
                     PGYSTIDNGMVIDLSHMKSITFDPERRIARLEPGLTWGEVAGTLQPYGLALTSGDTAT
                     VGVGGLLLGGGIGWMVRKYGLTIDHLKAVELVTAEGRLLRASAGENAELFWGLRGGGG
                     NFGVVTAYEVSLHPAGMVIGGAVIYDAADAEGILKAYARFAEAAPDELTTIVNFMAAP
                     PAPFIPPEKQGMPVLVIMVCYTGDLAEGERVVAPLRFLGTPIADIISPMPYPAMFAMT
                     EEASILGRQHTVRSLFMRTLSDDAIHTMVREAAAVVSPDTLVQVRVLGGAISRVPATA
                     TAFAHRDKQAMVMIASSVPVSADAELSRARAERVWQAMRPYADGVYVNFLADEGDRRI
                     HEAYPPATYARLAALKKLY"
     gene            1697..>2015
                     /locus_tag="VGT44_06040"
     CDS             1697..>2015
                     /locus_tag="VGT44_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2580396.1"
                     /translation="MGSDEATKEPAAQAMSHRFIETNGLKMHIVEQGTGPLVLLCHGF
                     PEFWYSWSHQVPALAAAGYHVVAPDQRGYGQTGKPGAIESYTLLHLVGDMLGLLDALG
                     EQQA"
BASE COUNT          430 a          571 c          560 g          454 t
ORIGIN      
        1 tcataaagct tcttgagcgc cgccagccgc gcatacgtcg ctggtgggta ggcttcatgg
       61 atgcgccgat caccctcgtc agcaaggaag ttcacgtaca ccccgtcggc gtagggccgc
      121 atggcctgcc agactcgctc agcgcgggca cgggaaagct cagcgtcggc tgaaaccggc
      181 acggaactgg cgatcataac catagcttgt ttgtcgcggt gggcaaaggc ggtcgcggtc
      241 gcgggtaccc ggcttatggc gccacccagg acgcgaacct gcaccaacgt gtcgggagag
      301 accacagccg cggcctctct gaccattgta tgaatggcgt catcgcttaa tgtccgcatg
      361 aatagcgaac gtacagtgtg ctgtcgccca aggatcgagg cctcctcggt catagcgaac
      421 attgcaggat aaggcatcgg cgagatgatg tctgcgatag gcgttcccag aaaccgcaga
      481 ggcgccacaa ctcgctcgcc ctccgcgagg tctccggtgt aacacaccat gatgacgagg
      541 actggcatgc cttgcttctc tggtgggatg aaaggcgctg gcggggcagc catgaaattt
      601 actatggttg tgagttcatc tggcgccgcc tctgcgaagc gcgcataggc cttcaagatg
      661 ccttcagcat cagccgcatc gtaaatgacc gcaccaccta tcaccattcc ggcagggtgc
      721 aagctgacct cgtaggcggt gacgactccg aagttgccac ctcccccacg cagtccccag
      781 aacagctcgg cgttttcgcc cgcgctcgcg cgcaagagcc gaccctcagc ggttaccagt
      841 tcaacggcct tcaggtggtc aatggtcaga ccgtatttgc gtaccatcca accaatccca
      901 ccgccgagca gcagaccacc aacaccgact gtggctgtat cacccgaggt aagagccagt
      961 ccatagggct gtaacgtccc tgctacctca ccccaggtga gtcctggttc tagccttgca
     1021 atgcgtcgtt ccggatcgaa agttatagat ttcatatggg agagatctat caccattcca
     1081 ttatcgatgg tgctgtaccc tggcatacta tgacctccgc tacgcacagc cacctctacg
     1141 ccctgttcgc gcgcaaagtt gacgctactg atgacgtcct ctacgccggc tgggcgtgca
     1201 atcagggcag gatggcgatt acccacgccg ttccagacga ctcgcgctga ctcatagtca
     1261 gggtcaccgg ggaagagtat ctcaccgtga aagcgtgata ctaactcccg tatagcctgc
     1321 cggtaggcac gttgactttc tctctcggac atctttttag caaacatgtg cctatcctgg
     1381 ttagcattgt tttgagcatt ctgcaacata ggtttttcct tcattggttt cataggcatt
     1441 gctatttttt gatgtgtttt atatccctgg ataaataggt actatatgca gtactcttta
     1501 tcatcatggt aagcgtaata tctgttccgt ttcttaagcg tttctccgct agctatctct
     1561 ggtaggcaca ggggattgag cgtaagcgag acgggaacgt gaaaattgat tcggcaatta
     1621 gcgtagtggt agaatgcaga ggtcaatgga gattacttct tcacatgctt tgctatagat
     1681 acgtacagga gattgtatgg gcagcgatga ggcaaccaaa gagcccgcgg cacaggcgat
     1741 gtcacaccgg tttatcgaga ccaacggcct gaagatgcac attgtcgaac agggcacagg
     1801 gcccctggtg ctgctctgtc acggctttcc cgagttctgg tattcgtgga gccaccaggt
     1861 cccggctctg gcggctgcgg gctatcatgt cgtggcgcct gaccagcggg gatatggcca
     1921 gaccggcaaa cccggtgcga ttgagtccta tacactcctg cacctggtcg gtgacatgct
     1981 gggtttgctg gatgcgcttg gagagcaaca ggcgg
//
LOCUS       DASZAK010000344         4465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_80031, whole genome shotgun sequence.
ACCESSION   DASZAK010000344 DASZAK010000000
VERSION     DASZAK010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80031"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VGT44_06045"
     CDS             complement(<1..921)
                     /locus_tag="VGT44_06045"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV2580397.1"
                     /translation="MSLTHYNIAPELTDSYIDAVWETLPLHIRPTLLLPAPVLSARLG
                     CEITIASETFQYTGSFKFRAAYNLLSSVPEQRVVTASSGNFGQAVAYACRLLGKTCTV
                     VMPSTSASVKIAAVQANGGIVDLVDVQTVSRNERVQQLLFEQPGAFQAQAYDDYRVVA
                     GNSTLTKEILGAADVDFIVVPVGGGGLSSGQIIARDHLNACAEIIGAEPQPGNDAARS
                     LRSGTRLFNDQEPTTVADGARTLGLGELNWEIIRRGMADIIEVPDAQSLEALRQYFTF
                     ANLKVEPTGALSLGAIMASPARFQGKKVCCI"
     gene            complement(959..1951)
                     /locus_tag="VGT44_06050"
     CDS             complement(959..1951)
                     /locus_tag="VGT44_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580398.1"
                     /translation="MGSYSNGQSSFTLVEHWNGTQWAVVASPNVAGSTSDELLSVVAV
                     TANDIWAVGDSSNMQKTLIEHWNGAQWAVVASPNFSPTGNSLASISIDSATDIWAVGT
                     TTSTSSSSGYQPLIELWNGTTWSISSSPVLSGRLFSVVAIKSNDVWAVGADIMPNVTD
                     TLIEHWNGAQWSTVSGANPGAASNSLNAVVNVTGGNIWAAGDYSNSVGPKADYTPLVE
                     YWNGSKWGALNSPLQGTSDLINGMATSSSSDITIVGDYRAGIDPMGPYYTLVEHWNGT
                     LWSVVNSPSPGSITSDLLAAANVANSGAAWAVGSTYGLNSGQNNIYQTLTEFHC"
     gene            complement(1938..2267)
                     /locus_tag="VGT44_06055"
     CDS             complement(1938..2267)
                     /locus_tag="VGT44_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580399.1"
                     /translation="MYRARQLLWHEYNWPPIDTNARRANTACDEDNFAIANCDAYSFG
                     ITNYEKYSFTIANCDEHSFANTSADRFPRCLCGLEDGFQSKCGDERYLTWSGCPFHER
                     RLGGGIL"
     gene            2587..3000
                     /locus_tag="VGT44_06060"
     CDS             2587..3000
                     /locus_tag="VGT44_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HEV2580400.1"
                     /translation="MSESEEITAMIRVRMSANDAHYGGQLVNGAHMLSLFGDLATELA
                     IICDGDEGLLVSYEGVEFKAPLYAGDFVEAYGRITHIGRTSRRMEFECRKVITARPDL
                     SDSAADVLDQPILIGRAVGTTVVKADRQRKTQTAS"
     gene            3073..3681
                     /locus_tag="VGT44_06065"
     CDS             3073..3681
                     /locus_tag="VGT44_06065"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2580401.1"
                     /translation="MQTETRTVRAKILAAAVQLFAEYGYHASTMRDIARATGIQAASI
                     YYHYDNKQALLVEIMDTHMRNLNANLERIVQSSSPLLQRLREAIANHIRLHTTYKAEF
                     FIIDTEIRALERENRAAILAQRDHYEDMLQTLLREGMNQELLRRFDIKIASYALIAMC
                     TEVATWFRPEGRLSVQQVIDIYTDMITEGLLLKGTSEQTQEE"
     gene            complement(3629..>4465)
                     /locus_tag="VGT44_06070"
     CDS             complement(3629..>4465)
                     /locus_tag="VGT44_06070"
                     /inference="COORDINATES: protein motif:HMM:NF028222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2580402.1"
                     /translation="DAVVVANSAPCLQCFYCQRQRYSLCENLLLLNGAYAEYLLVPER
                     IVQQNLYKLSAVTSFIAAALTEPLACALHGIAASDLHPGDTVAILGAGPLGLLLIALA
                     SQRGVRVISSGRGVERLNLARLYGADVVIDAGELSLEEQKQAVVEQTEAGRGADVVIE
                     AIGSPETWGLAAQLARPGGLVIFFGGCAAGTRVSLETYPLHYSELTLKGVFHHTPRFF
                     AQALEYIQCEHIDVEALVTAHLPLSKAVDALDLLLQKRGIKYALIPPAFAQMFLSAAN
                     PP"
BASE COUNT          997 a         1251 c         1256 g          961 t
ORIGIN      
        1 gatacaacag acctttttcc cctggaaacg cgcgggtgat gccataattg cgcccaagga
       61 gagtgcgccg gttggttcta ccttgaggtt ggcaaaggtg aaatactgcc gcagcgcctc
      121 cagcgactgc gcatctggca cttcaattat atctgccatg ccgcgccgga tgatttccca
      181 gttcagttcg ccgaggccaa gtgtgcgcgc gccatcggcg accgtcgtcg gctcctggtc
      241 gttgaagagg cgcgttccgc tgcgcagcga acgcgcggca tcattgcccg gctggggttc
      301 cgcgccgatg atctccgcgc aggcgttgag atgatcgcgc gcaatgatct gaccggagct
      361 gagaccgcct ccgccgaccg gcacaacgat gaagtcaaca tctgctgcgc cgagtatctc
      421 tttggtcagt gtcgagttac cggccacaac gcgataatca tcgtatgcct gtgcttgaaa
      481 cgcgcccggc tgctcaaaca acagctgctg cacgcgctcg ttgcggctga ccgtctgcac
      541 atcaaccagg tcaacgatgc cgccgttggc ttgcaccgcc gcaatcttga cgctggccga
      601 agtcgatggc atcacgaccg tacacgtctt gccaagcaaa cgacaggcat aggccaccgc
      661 ctgcccaaaa ttccccgacg aggcggtcac gacgcgctgc tcaggaacgc tggagagcag
      721 gttataggcg gcgcgaaact tgaagctgcc ggtatattga aacgtctcgc tggcaatcgt
      781 aatctcgcag cccagcctgg ccgatagaac cggggcaggc aggagcaacg tggggcgaat
      841 gtgcagcggt aaggtctccc agaccgcatc aatgtaagag tcggtcagtt ctggtgctat
      901 gttgtagtgc gtaagggaca tgagaaactc cgctagagat acaatgaatt ccctcgtatt
      961 aacaatggaa ctcggtcaag gtctggtaaa tgttgttctg cccactgttc aaaccatatg
     1021 tggagccgac agcccaggcg gcaccactat tcgctacatt tgcggctgcc agcaagtcac
     1081 tggtaatcga gccaggactt gggctgttta ccacgctcca cagtgtgcca ttccaatgtt
     1141 caactaacgt atagtacgga cccatcggat cgatgcccgc ccggtaatct ccaacgatgg
     1201 taatgtcgct ggaggaggac gtggccatcc cattgatcag gtctgaggtt ccctgaagtg
     1261 ggctatttag tgcgccccac ttcgagccat tccagtattc aacgagcggg gtataatctg
     1321 ctttaggacc aacgctattc gagtagtctc ctgctgccca gatgttaccg cctgtgacgt
     1381 tgacaaccgc gttgagacta ttggatgctg ctcccggatt tgcgccgctg acggtactcc
     1441 attgagcgcc gttccaatgc tcgatcaggg tatccgtgac gtttggcata atgtcggcgc
     1501 caaccgccca gacatcgttt gatttgatag ccacgacact gaaaagtcgg cccgaaagaa
     1561 ccgggctgct gctgatgctc cacgttgtgc cgttccagag ttcgatcagc ggctgatagc
     1621 cactcgaact gctcgtgctt gtagtcgtgc cgaccgccca gatgtccgtt gccgaatcta
     1681 ttgaaataga ggcaagcgaa tttcccgtcg gcgaaaagtt tggactggca acaacagccc
     1741 actgcgcgcc gttccagtgt tcgatcagag tcttctgcat atttgagcta tcccccaccg
     1801 cccagatatc gttcgctgtg acagcaacca cgcttagcag ctcatcgctg gtacttccag
     1861 cgacgtttgg actggcaaca acagcccact gcgtgccgtt ccagtgttcg acgagcgtga
     1921 agctgctctg accattgcta taggatccca ccgcccagac gtcgttcgtg gaaaggacag
     1981 ccactccatg taaggtagcg ctcgtcccca catttggact ggaaaccgtc ctccaggccg
     2041 cacaggcacc tggggaaccg gtcggctgag gtgttggcaa agctgtgttc gtcgcagttg
     2101 gcgatggtga agctgtactt ttcgtagttg gtgatgccga agctgtatgc gtcacagttg
     2161 gcgatggcaa agttgtcttc gtcgcaggcg gtgttcgcac ggcgggcgtt ggtgtcgatg
     2221 gggggccaat tgtactcgtg ccacaggagc tgacgagcgc gatacaacag ctgatgacca
     2281 gaaataggcg ataacttgtt ttccggggtt tcatcgagtc ttgcctggct aacctctcgt
     2341 caacctccaa ttctttggca atctgttttt ttctaaacga cattccattt tgcgtcatgg
     2401 gcgcactcct aaacggcttt agtgttcata gtaccatatc ggtcaaaatt tctactcatg
     2461 agtaaacaaa ggcgcgttag gcaatggcta gacacaagaa agaacctcaa gtatactctt
     2521 tgagattgaa gctgtcagac cacgacgtac atctctgctg agaacagcaa ggaagaggag
     2581 cctatcatgt ccgagtcaga agaaatcact gcgatgatcc gtgtgcggat gagcgcgaac
     2641 gatgcccatt atggcgggca actggtcaat ggagcgcata tgctgtcact ttttggagat
     2701 ctcgcgaccg agttagcgat tatttgcgat ggggatgagg gtctgcttgt ctcctacgag
     2761 ggcgtcgaat tcaaagcgcc attgtacgcg ggcgattttg tcgaagccta tggccgcatt
     2821 acgcacatcg gtcgcacatc gcgccgcatg gaattcgagt gccgcaaagt gattaccgcg
     2881 cgtcctgatc tcagtgactc ggcggctgat gtcctcgatc agcccatctt gatcgggcgc
     2941 gccgtgggaa cgaccgtcgt caaggccgac cgccagcgta aaacgcaaac ggcatcatag
     3001 ttgcgttatc gagtcgctct gttcgcataa atctgctata ctgcgagaaa cacagtccag
     3061 ggagaacaat ctatgcagac agagactcgc acggtacgcg cgaagatact cgcagctgcg
     3121 gttcagcttt ttgcggagta tggctaccac gcatccacca tgcgtgatat cgcgcgcgcg
     3181 accggcattc aggccgccag catctactat cactacgata ataaacaggc gctgctggtt
     3241 gagattatgg atacccatat gcgcaacctg aacgccaacc tggagcgcat tgtacaatca
     3301 tcgtcccctc ttttacagcg actgcgagag gcgattgcca accatattcg cctgcatacc
     3361 acctataagg cagagttttt catcatcgat acggagatac gcgcgctcga aagagagaat
     3421 cgcgcagcca tcctcgctca gcgcgaccat tacgaggaca tgctgcagac gctgctgcgc
     3481 gagggcatga atcaagaact gctgcgccgg ttcgatatca aaatagcttc ctacgcgctg
     3541 attgctatgt gtaccgaagt agcgacatgg tttcgaccgg agggacgcct gagcgtacag
     3601 caggtgatcg acatctacac agacatgatt acggagggtt tgctgctgaa aggaacatct
     3661 gagcaaacgc aggaggaatg agcgcgtact tgatgccgcg cttttggagc agtaaatcca
     3721 gcgcatcgac ggctttggat aaaggaagat gggcggtaac gagcgcttct acatcgatgt
     3781 gttcacactg gatgtactcc aatgcctggg cgaagaaacg aggtgtgtgg tggaaaacgc
     3841 ccttgagcgt caattcgcta taatgcaggg ggtacgtttc aaggctgacg cgcgtaccgg
     3901 cagcgcaccc gccaaagaaa ataaccagac cgccggggcg agcaagctgc gcggccagtc
     3961 cccatgtttc cggcgatccg atcgcttcga tcaccacatc cgcgccgcgc ccggcttccg
     4021 tttgctccac gacagcttgc ttctgctcct cgagggaaag ctcgccggca tcgatcacga
     4081 catctgcgcc atacagacgc gccaggttca aacgttccac tcctcgtccg ctggaaatta
     4141 ccctgacgcc gcgctggctc gccagcgcga tgagcaatag cccgagcggg ccagcgccga
     4201 ggatggcgac ggtatcaccg ggatgcaggt cgctggcggc aatgccatgc agggcgcagg
     4261 cgagcggctc ggtcagcgct gccgcgatga atgaggtgac tgccgatagc ttgtagaggt
     4321 tctgctgcac gatgcgctca ggaaccagca ggtattccgc gtacgcgccg ttaagcaaga
     4381 gcaggttttc gcacaggctg tagcgctgcc tctggcaata aaagcattgc aggcatggcg
     4441 ctgaattggc cactacgacc gcgtc
//
LOCUS       DASZAK010000345         3193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_576863, whole genome shotgun sequence.
ACCESSION   DASZAK010000345 DASZAK010000000
VERSION     DASZAK010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_576863"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..2087
                     /locus_tag="VGT44_06075"
     CDS             165..2087
                     /locus_tag="VGT44_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580403.1"
                     /translation="MTIHSGGPHLLLFERDQQLTAVLMSELQLAGYECHAARTAVEVF
                     DAIARFPIRLVLVNLAQAAAGRREFWVALDAQRRGRGVQVFTFRCMNIAGYGVDDPDE
                     RARIVLTDMEVDGMLGVMNLVDAVRARIPAAPSASSTGSFARVGASPLSRDSARLEAI
                     RPVSQPGFGAVPMPVPPQIVQAAQAAQTVSQATAPLSALNPTPSAPMPAVPSASALSA
                     SAQPVAPPVPSQQQAASSTNLPQGAGMQPTTFTDKIRAVLRPGLVAVSPNPEANWVPF
                     ATPSAAGRRLDRPETENAALHSINGTTVAGQQAQNLPPVVDGAPAPVQQGEETGLAQL
                     SRMVQDTVSDELPLRASPIEDLPVERLTERKNGSVGTTMAEAARHAPQLESINTSTVE
                     TMGEKRRALPIEEALPAMSPPSSAVDRAQVDQVNVSTTRREGEERIDAVLVEQVEQTT
                     PENVARPEIAVTTAMPEVPGMPGISEALTAAALRRRSEHSVTSEDVLLNIVQSLPPMN
                     ALPTPAQPASPQVAVLSGRATRSLANVLIDGHLVPQDRLEVAQSIQRMLRGVDMNYQL
                     GEILLMFKLLTPDQLLAASLVSYGMISAVQISALGRIRQELHSMGLEYDLESLLVLFR
                     ILSSDQLREVRSSWSG"
     gene            complement(2098..>3193)
                     /locus_tag="VGT44_06080"
     CDS             complement(2098..>3193)
                     /locus_tag="VGT44_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580404.1"
                     /translation="FARVGYLMFLRPIPAHERALPFDQWLARHINTERNPELVAFFER
                     ISRFALSLELRQVSTDEVVRTTKNMFRYGAPGIVEGGCAALTTELQRRVTGQGASLRL
                     QYDAQQILYKDGQVTGVRARDKTSSAEHILRAPLVVSDLGPRATDALLHNRQITQAEV
                     LKQPTEYQEAIGLKVHLLSDISLIPHKGIMYCLDTQRIAGIVQPTNCDARLAPPGKHL
                     VITHQVMQSSNVEEEKALALADLRSIFGEAFEQHCRVLTISAYRGEWPVNRAAQGEDV
                     NPQTSIAGLYLVGDAVKPSGYLMVEGVAQSVNILLDLLDAVEQGNSDGPLGAPTIRRP
                     RHIVSPPSRLRVLRWLWEAPGNSKTIKSRL"
BASE COUNT          614 a          927 c          995 g          657 t
ORIGIN      
        1 gaaagtaatg ggttgccagg aagcgctagt attcaggata ttgtatcacc tgagtcccca
       61 aatcttctct aagactatgc cacgagcgtg tttgtgtgtt atactagcgc agtcaaaaaa
      121 gtaccgataa tggtcagagg aactgcaacg caaggagacc cggcatgacg atacatagcg
      181 gtggcccaca ccttctttta tttgagcgcg atcagcagct cacagctgtg ctgatgagtg
      241 agttgcaact ggccggatat gagtgtcacg cggcccgcac ggcggtcgag gtatttgatg
      301 cgattgcgag atttccgatc cggctggtgc tggtcaacct cgcccaggct gcagcgggca
      361 ggcgcgaatt ctgggtggca ttagatgccc agcgacgcgg acgcggcgtg caggtcttca
      421 cctttcgatg tatgaacata gccggatatg gtgtggatga tcccgatgag cgcgctcgta
      481 tcgtgctgac cgatatggaa gtggatggaa tgctcggcgt catgaacctg gtagatgcgg
      541 tgcgtgcgcg aattccagcc gccccgtcgg cctcatctac cggctcattc gcacgggtgg
      601 gcgcttcgcc gcttagccgt gattccgcca ggcttgaggc catcaggccg gttagccaac
      661 cgggattcgg ggcagttccc atgcctgttc cgcctcaaat cgttcaagca gctcaggccg
      721 ctcaaacagt ttcgcaggca accgcgccgc tatcggcctt gaatcccacg ccatctgctc
      781 ccatgcctgc ggttccctct gcttctgcgc tctctgcttc cgcgcagccg gttgctccgc
      841 ctgttccatc acagcagcag gctgcttcat cgaccaacct tcctcagggt gctggtatgc
      901 aaccaacgac gtttaccgac aaaattcgtg cggtgcttcg tcccggtctc gtggccgttt
      961 ccccaaatcc agaggcgaac tgggtgccat ttgcaacgcc gtccgctgcc ggtcgccgtc
     1021 tggatcgccc ggagacggag aatgccgcgc ttcattccat caatggaacg actgtggctg
     1081 ggcaacaggc gcagaatcta ccgcctgttg tggatggtgc ccctgcgccg gtccagcaag
     1141 gcgaggagac tggcctggcc cagctttcgc gtatggtaca ggacacagtg tccgacgagc
     1201 ttcccctgcg ggcgtcgccc atagaggacc tgcccgttga gcggttaaca gaacgcaaga
     1261 atggatcggt tgggactaca atggcggaag cggctcgtca tgccccgcaa ctcgagtcaa
     1321 tcaatacgtc gacggttgag actatggggg agaagcgaag ggcattgccc atcgaggaag
     1381 cgctaccggc catgtctcca ccatcatcgg ctgtagatag agcgcaggtc gatcaagtca
     1441 atgtcagcac gactaggcgg gaaggcgaag agcgaatcga tgctgtcctc gtcgagcaag
     1501 tagagcaaac cactccggag aatgtagcca ggccggagat cgccgtaacg acggcaatgc
     1561 cagaggttcc agggatgccg ggcatatcgg aggcgctgac ggcggcggct ctgcgcaggc
     1621 gttcggaaca cagtgtgaca tcggaggatg tgctgctcaa tattgtgcag agcctgcccc
     1681 ccatgaacgc gctgcccacc cccgctcagc ccgcgtcgcc gcaggttgct gtcttgagcg
     1741 ggcgcgctac ccgctcgctg gctaacgtct tgatcgacgg gcatctggtg ccgcaggatc
     1801 gcctggaagt ggcgcagagt atccagcgca tgctgcgtgg cgtggatatg aattaccagc
     1861 tcggcgaaat tctgctgatg ttcaagctgc tgacgcccga tcaactgctg gccgccagcc
     1921 tcgtcagcta tggcatgatc tctgccgtgc agataagcgc actgggtcgc attcgccagg
     1981 aactccattc gatgggcctg gaatatgacc tggagagcct gctggtactc ttccgtatac
     2041 ttagctcgga tcagcttcgc gaggtgcgct ccagttggag cgggtagtga tacgccccta
     2101 cagccgggac ttgattgttt tggagttgcc gggtgcttcc cagagccagc gcaggacgcg
     2161 caggcgcgag ggcggtgaga cgatgtggcg tgggcgacgg atggtaggcg cgccgagggg
     2221 accgtcgctg ttgccctgct cgacggcatc taggagatcg agcaggatgt tgacgctttg
     2281 cgcgacgcct tcgaccatca gatagccgct gggtttcaca gcatcaccaa ccaggtagag
     2341 gcccgcgatg gaggtctggg ggttgacatc ctcgccctgg gccgcgcggt ttaccggcca
     2401 ttcaccacga taggcgctga tcgtcaggac gcggcaatgc tgctcgaacg cctccccaaa
     2461 aatcgagcgc aggtcggcca gcgccagggc tttctcttcc tccacgttgc tgctttgcat
     2521 gacctggtgg gtgataacca ggtgcttgcc cggcggcgcg aggcgcgcgt cacagttggt
     2581 cggctgcacg atgccggcga tgcgctgtgt gtccaggcaa tacatgatgc ctttgtgtgg
     2641 gatcagcgag atatcgctca gcaggtggac tttgagaccg atcgcctcct gatactccgt
     2701 aggctgtttc agtacctcag cctgggtgat ctggcggttg tgtagcagcg catccgtggc
     2761 gcgcggccct aaatcgctca cgacgagcgg ggcgcgcagg atatgctccg cgctgctcgt
     2821 cttgtctctg gcgcgtaccc ctgtgacctg gccgtctttg tacaagattt gctgggcatc
     2881 gtattgcaat cgtaatgaag caccctgccc ggtgacacgg cgctgtaatt ccgtcgtgag
     2941 cgccgcacag ccgccttcga cgatgcccgg cgcgccataa cgaaacatgt tcttcgtagt
     3001 gcgaaccacc tcatcggtgc tgacctggcg gagttccagg ctaagcgcga aacgcgagat
     3061 gcgctcaaaa aaagcgacca gttcggggtt gcgctctgta ttgatgtgac gtgccagcca
     3121 ttgatcgaac ggcagcgcgc gttcatgagc gggaatcggt cggagaaaca tcaggtagcc
     3181 gacgcgagcg aac
//
LOCUS       DASZAK010000346         1897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_328447, whole genome shotgun sequence.
ACCESSION   DASZAK010000346 DASZAK010000000
VERSION     DASZAK010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328447"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..1388
                     /gene="hflX"
                     /locus_tag="VGT44_06085"
     CDS             108..1388
                     /gene="hflX"
                     /locus_tag="VGT44_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV2580405.1"
                     /translation="MEHAFLVAVEQDESDSWWSVEDSLGELGTLARTAGAEVVGTMIQ
                     RMHHPDVATYIGKGRAQELSDTEKQLGFDLVIFDDELSPSQQRNLEKLLDARVIDRTA
                     LILDIFAQHARTREGRLQVELAQLEYRLPRLAGRGVQLSRQAGGSRSAGAGGVGGAIG
                     VRGPGETKLELDRRRIRSRIAELREEIEAVREQRKVHRRQRAAQAIPVVAVVGYTNAG
                     KSTLFNALTEAEVLTEDKLFATLDPTTRHVVLPGNQEALLTDTVGFIQKLPTKLIAAF
                     RATLEEVVDADLLLEVVDVSHENAIEQSETVNDVLHGLEAGDKPRVTALNKIDLLDDP
                     SEVDTSLYPNAVAVSALHKAGLDALREKIAQVLAASMEAIEVTIPYDKSELVELFHRR
                     GHVEREEHLPEGTLLAGRLPCSLGGYYTRYKRAQ"
     gene            1700..>1897
                     /locus_tag="VGT44_06090"
     CDS             1700..>1897
                     /locus_tag="VGT44_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580406.1"
                     /translation="MVDTARFLVRFWGVRGSYPTPGPHTVRYGGNTACVEVEVGKHTL
                     ILDAGSGIIRLGNELLRRSKGQ"
BASE COUNT          426 a          485 c          567 g          419 t
ORIGIN      
        1 ggcaattcac gatcgcctac gcgagatcgc gttcgccgag cgcaagaccg tcaccgatct
       61 aatcaacgag ggcgtggagc acgttctgca gaatagggga tttgcgggtg gaacacgcct
      121 tcctggtagc tgtagagcaa gacgagagtg acagttggtg gagtgttgaa gactcattgg
      181 gtgaattggg aacactggca cgtaccgctg gggccgaagt tgtcgggact atgattcaac
      241 gcatgcatca tccagatgta gcgacctata taggaaaagg acgtgcgcag gaactgagtg
      301 ataccgagaa acaactcggt ttcgacctgg tcatcttcga tgacgaactt tccccctcgc
      361 agcagcgcaa ccttgaaaag ctgctagatg cgcgcgtgat tgatcgtacc gcgctgattc
      421 tcgatatctt tgcgcaacat gctcgtacgc gtgagggacg cttgcaggtt gagctggcgc
      481 agttggagta tcgcttgcct cgcctggccg ggcgcggtgt gcagctttca cgccaggcgg
      541 gcggttcgcg aagcgccggc gcgggtggcg ttggcggcgc tatcggtgtg cgcggtcctg
      601 gtgaaacaaa gctggagctt gaccggcggc gtattcgcag ccgtatcgct gaattacgcg
      661 aagagatcga agcagtgcgc gagcagcgta aagtgcatcg caggcagcgt gcggcccagg
      721 ccattcctgt cgtagccgtg gttggctata ccaacgccgg aaagtctacc ctcttcaatg
      781 cactcactga agccgaagtg ctgacggagg ataagctctt tgccacgctt gaccccacta
      841 cccgtcatgt tgtgttgccg ggcaatcaag aagccctgct caccgatact gtggggttca
      901 tccagaaatt gcctaccaag ttgatcgctg cttttcgagc gacactcgaa gaggttgtcg
      961 atgccgattt gctgctggaa gtggtggatg tcagccacga aaacgccatc gagcagagtg
     1021 aaacggtcaa cgatgtcctg catggcctgg aagccggtga taagccgcgt gtcacggctt
     1081 tgaacaagat cgatctgctt gatgacccgt cggaagtcga tacctcgctc taccccaatg
     1141 cagtcgccgt ttctgccctg cacaaggccg ggttagatgc gctgcgcgag aaaatcgccc
     1201 aggtgctggc tgccagtatg gaagcgatag aagtgacgat cccttacgat aagagcgagc
     1261 tggtagaact ctttcaccgc cgtgggcatg tagagcgcga ggagcacctt cccgagggga
     1321 cactgcttgc cggccgcctg ccgtgctcat taggggggta ttacacgcgg tacaaacggg
     1381 cacaatgata gattttgaca acataatcgg acaaccagta ggccgatcat ggattgtaag
     1441 tttttaacct ggatattcga tgaggcacgg gagccgaaaa tgtactttca aaaattaata
     1501 ccgattatga aatcagcctg tacgctgcta tggcgtcttt tataatccac atttattttc
     1561 gaaactgcat aatcggcgct gggcacggag tattgatacg cgctccgctt cgcttcgctg
     1621 aggcccccta ctttcctctt ttgcgatata ataagtgcaa atcgacgaat ctcgttaggg
     1681 cagtcgtagg agcaaccata tggtcgacac ggcgcgtttt ctggtccgtt tttggggagt
     1741 acggggcagc tatccaaccc ccggaccgca cacggtgcgc tatggaggaa ataccgcctg
     1801 tgtcgaagtc gaggtaggga agcatacgtt gatcctggac gcggggagcg gcatcattcg
     1861 cctgggtaat gagcttttgc gacgttcgaa gggccaa
//
LOCUS       DASZAK010000347         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_257846, whole genome shotgun sequence.
ACCESSION   DASZAK010000347 DASZAK010000000
VERSION     DASZAK010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257846"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /gene="phaZ"
                     /locus_tag="VGT44_06095"
     CDS             complement(<1..806)
                     /gene="phaZ"
                     /locus_tag="VGT44_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HEV2580407.1"
                     /translation="MQSESTRPKTDIRTITVKGQRLRVGIRPGNGTRTPLVLMNGIGA
                     NLEVLEPFVNALDPAFEVIRFDVPGVGGSPAPIIPYRFSALAWLVTKMLDQLGYNQID
                     VLGVSWGGALAQQFAFQYPRRCRRLILVSTGTGALMVPGRLPVLAKLATPRRYQDPSY
                     LMDIAGELYGGSVRTQPEYARAFARSMRQGDPLGYFYQMLGGIGWTSLPWLRFIRQPT
                     LIMSGDDDPIVPPINAKIMHHLIPRSQLYIFHDGHLGLGAKAKELAKVVE"
     gene            complement(823..>2423)
                     /locus_tag="VGT44_06100"
     CDS             complement(823..>2423)
                     /locus_tag="VGT44_06100"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2580408.1"
                     /translation="GDIFSVRRQIVEQAAKQPPLALKHYTNFLLEMSRVMTGQSTVEP
                     DAKDKRFVDPVWKTNPFYRMALQSYLTWRESLNGFINDSELGKKDAGRARFVLSLFTE
                     AVAPTNTLLGNPAAMKKMHETGGASLVRGLTHMIEDLANNGGLPSQVDMKAFQVGKDL
                     ATAPGAVVFKNEVLELIQFKPTTPQVYSRPMLIVPPQVNKYYMMDLSPGKSLVQYVVA
                     SGIQVFTISWRNPTPAHRDWGFETYDQAIMEAMAVVRAITGSEDVNISGACLGGMTLA
                     MLLGHLAAKGDRRINTATFLVTVLDSNVESTMGLFATKETIEAAKQAVRLKGVIEGQE
                     MARMFAWLRPNDLIWNYWVNNYLIGNDPAAFDVLYWNNDTTRLPAKIHGEMLDLFEAN
                     AFTHPGTLNLLGTPIDLSKVTCDAYLIAGITDHITPWQGCYATTQLLGGKCEFILSNS
                     GHIQAILNPPGNPKASYFVNSRYPADPEKWLAGAQKRSGSWWEDWRDWLGKRSGEQKP
                     APQELGNDQYQPGAPAPGTYVFEP"
BASE COUNT          489 a          723 c          685 g          526 t
ORIGIN      
        1 ttctcgacga ctttcgccag ttccttcgct ttggccccca gtcccaggtg tccatcgtga
       61 aagatgtaca gctgtgaacg tggaatcagg tgatgcatga tctttgcgtt gattggcggc
      121 acaatcgggt cgtcatcgcc agacatgatc agcgtgggct gtcgtataaa acggagccac
      181 ggcaggctgg tccaacctat tccccccaac atttgataga aatagccgag tgggtctccc
      241 tggcgcatag aacgagcaaa cgcgcgcgca tactcgggtt gcgtcctcac gctcccacca
      301 tagagctctc cggctatatc catcagatag gaggggtcct ggtagcgtct tggcgttgcc
      361 agtttggcca ggacggggag gcgacccggg accatgagcg cgcctgttcc cgtactgacc
      421 aggatcagcc gccggcagcg cctgggatat tggaaggcaa attgctgagc aagtgcaccg
      481 ccccacgaga caccaagcac atctatctgg ttgtaaccca attgatcgag catcttcgtc
      541 accaaccagg ccagggccga aaaacggtac gggataatcg gcgctggcga accaccaacc
      601 cccggcacat cgaagcgaat gacttcgaag gccggatcga gtgcgttaac gaacggctcg
      661 agcacttcaa ggttggcacc gatcccgttc atcagcacga gtggcgtgcg ggtgccgttt
      721 cctggacgga tgccgacccg caggcgctgc cctttgacgg taatagtacg aatgtcagtc
      781 tttggcctgg ttgactctga ttgcacggat gttcctttca cttcatggtt caaatacgta
      841 ggtccccggt gccggtgctc ccggctgata ctggtcgttg ccgagttcct gcggcgcggg
      901 cttctgctcg ccggatcgct tacccagcca gtctcgccaa tcctcccacc aggagcccga
      961 gcgtttctgc gctccggcaa gccacttctc aggatcagcc gggtagcgtg aattgacgaa
     1021 gtatgacgcc tttgggttcc ccggcgggtt caggatagcc tggatatgac cactgttgct
     1081 caagataaac tcgcacttgc cgcccaacag ttgcgtggtc gcatagcagc cctgccacgg
     1141 tgtgatatgg tcggtgatgc cggcgatgag ataggcatca cacgtcacct tggagagatc
     1201 gataggcgtc cctagcagat tgagcgtgcc aggatgcgtg aacgcattgg cctcgaaaag
     1261 gtcgagcatc tcgccatgga tcttcgcggg cagacgggtc gtgtcgttat tccagtacag
     1321 cacgtcgaac gccgccggat cattaccgat caggtaattg ttgacccagt aattccagat
     1381 cagatcgttg ggacgcagcc aggcgaacat tcgtgccatc tcctgaccct caatcacccc
     1441 cttcaagcgt accgcctgct tggcggcctc gattgtttct ttggtggcga acagacccat
     1501 cgtcgactcg acgttggagt caagcaccgt caccaggaag gtcgcggtgt ttatccgccg
     1561 gtcccccttc gccgccaggt gccccagcaa catggcgagg gtcatgccac ccaggcaagc
     1621 tcccgagata ttgacgtcct cgctgcctgt aatagcacgc accactgcca ttgcttccat
     1681 tattgcctgg tcgtaggtct cgaagcccca atcgcgatgt gccggcgtgg gattgcgcca
     1741 gctgatggtg aacacctgta taccactagc gaccacgtac tgcaccaggc tcttgcctgg
     1801 cgacaggtcc atcatgtaat acttgttcac ctgcggcggg acgatgagca tcgggcgact
     1861 gtacacctgg ggagtcgtcg gcttaaattg gatcagctca agcacttcgt tcttgaatac
     1921 gacagccccc ggcgcggtgg ccaggtcctt gccgacctga aaggccttca tatcaacctg
     1981 ggagggcagg ccgccgttat tggcgaggtc ttcgatcatg tgggtgaggc cgcgcaccag
     2041 gctggcaccg cccgtctcat gcatcttctt catcgccgcc gggttgccca gcagcgtgtt
     2101 ggtcggagca acagcttcgg taaacagcga gagcacgaag cgagcgcgcc ccgcatcctt
     2161 cttgccaagc tcggagtcgt tgataaagcc attcaggctc tcgcgccagg ttaaataact
     2221 ttgcagcgcc atgcggtaaa agggatttgt cttccacacc gggtcgacga agcgtttatc
     2281 cttcgcgtcc ggctcgaccg ttgactgacc ggtcatcacg cggctcatct cgaggagaaa
     2341 attcgtataa tgtttcagcg ctaatggtgg ttgcttagca gcttgctcga caatctgccg
     2401 cctaacgctg aagatatctc cac
//
LOCUS       DASZAK010000348         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_185190, whole genome shotgun sequence.
ACCESSION   DASZAK010000348 DASZAK010000000
VERSION     DASZAK010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185190"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VGT44_06105"
     CDS             complement(<1..903)
                     /locus_tag="VGT44_06105"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2580409.1"
                     /translation="MVDRTGEQIGNYRLIRLLGSGGFAEVYLGQQIYLDSAAAIKLLH
                     TNLAQDEWEAFRSEARTLVNLVHPHIIRLLDFGRDGNTPFLVMDYAPNGTLRQRIQRG
                     ERLPLATVVDYTNQIASALQYAHNEKVIHRDIKPENMLVGKNNEILLTDFGIAIVAAS
                     TRTQFTQETVGTIAYMAPEQIQAHPRAASDQYSLAVVVYEWITGSRPFNGAFTELAVK
                     HMMVPPPPLHETILDISPDVERVVLTALAKDPKERFGSVQAFAMALERAAKDALAIAD
                     QATLLPPPERAQQEQVARPAQPMQK"
     gene            1043..1447
                     /locus_tag="VGT44_06110"
     CDS             1043..1447
                     /locus_tag="VGT44_06110"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2580410.1"
                     /translation="MTPLHARRILNSMVNGSSEQLTTVFLALADPTRRAILERLAHGE
                     ASGTELAQPFSISVPAISKHLRVLEHADLILHRKDGRTHRFRLAARPMREAAVWLEHY
                     RRFWEEQFDALDTYLQVTSEEEQDPDDPFSIS"
     gene            1425..1874
                     /locus_tag="VGT44_06115"
     CDS             1425..1874
                     /locus_tag="VGT44_06115"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV2580411.1"
                     /translation="MIPSASPNNTTLVLRRTFTASRARVFRAWITPRALEHWLRPRGM
                     SMTVRSLDAQVGGSFHFDLENGSSIVGTYLQIVPPEKLVFTWSGRISPGHETVVTLDF
                     LDHGAVTEVVLTHEGLSSPEQRALAEGGWPSLLDALASVLSSPHLDF"
BASE COUNT          475 a          654 c          592 g          487 t
ORIGIN      
        1 cttctgcatc ggctgtgctg gccgcgcgac ctgctcctgc tgcgctcgtt cgggcggtgg
       61 caggagagtg gcctggtctg caatcgccag ggcgtccttc gcggcgcgtt cgagcgccat
      121 agcgaaggct tgcacgctgc cgaaacgctc cttagggtcc ttcgccagcg cggtcagcac
      181 gactcgctcc acatcgggcg aaatatcgag gatggtttca tgcagcggag gcggcggaac
      241 catcatatgc ttgaccgcca gctcggtaaa cgcgccgttg aacgggcgac tgcccgtaat
      301 ccattcgtaa acaaccaccg ccaacgaata ctggtcgctg gccgcgcggg gatgcgcctg
      361 gatttgttcg ggggccatgt aggcaatcgt gccaaccgtc tcttgcgtga attgcgtgcg
      421 cgtactcgcc gcgacgatcg caatgccgaa atcggtcagc aagatttcgt tattcttgcc
      481 caccagcata ttttccggct tgatatcacg atgaatgacc ttttcgttat gggcatattg
      541 cagcgcgctg gcgatctggt tggtatagtc aacgaccgtc gccaggggca aacgctcccc
      601 gcgctggatg cgctgtctca gggtaccgtt gggcgcataa tccatgacga ggaagggcgt
      661 gtttccatct cgcccaaaat ccagcaggcg aataatatgt ggatggacca ggttcacgag
      721 cgtgcgagct tcggaacgaa aagcttccca ctcatcctgc gccaggttgg tgtgcagcaa
      781 cttgatggcc gccgcgctat cgagatagat ctgctgaccg agatacacct ccgcgaaccc
      841 tccgctgccc agcaatcgaa taaggcgata gtttccaatc tgctcacctg tgcggtcaac
      901 catcgtgtct ctcctctagg attgcatgta caaatagcat gtaataacag tactacctct
      961 caagccagga aaatccgact ccattatagt ctaattttat cgatgcgaca actgaccctc
     1021 ttcgctcgcg gaaagcgggc cattgacacc gcttcacgct cgccgtatac ttaacagtat
     1081 ggttaatggt tcatcagaac aactcactac cgtctttttg gcgctcgctg accccacacg
     1141 ccgggccatc ttagaacgcc tggcgcacgg tgaggcttcg ggaacagaac tggcgcagcc
     1201 cttctcgatc tccgtgccgg cgatctccaa acatttacgg gtgctggaac acgcggatct
     1261 gatcctgcac cgcaaagatg gacgaacgca ccgctttcgc cttgctgccc gccccatgcg
     1321 agaggctgcc gtctggcttg aacactaccg gcgcttctgg gaagaacaat tcgacgcgct
     1381 ggatacctac ctgcaagtta catcagaaga ggagcaagac cctgatgatc ccttcagcat
     1441 ctcctaacaa caccaccctc gtactgcgac ggacgttcac cgcctcacga gcgcgcgtgt
     1501 tccgcgcctg gatcacaccc cgggcgttgg aacactggtt gagacccagg ggcatgagca
     1561 tgaccgtgcg ttcgctcgac gcgcaggtag gggggtcctt ccatttcgat ttagagaacg
     1621 gcagttccat cgtcggcacc tacttacaga ttgtcccacc cgaaaaactg gtctttacct
     1681 ggtcaggtag aatatcaccg ggccatgaaa ccgtcgtcac gctggatttt cttgaccacg
     1741 gtgcagtgac agaagtcgtg ctgacccacg aagggctgag ttctccagag caacgtgcgc
     1801 tggctgaagg tggctggcca tctctgctcg acgcgctcgc ctcggtactc tcctctcccc
     1861 atctcgactt ttgatgcgct gttcatactc ctgctccaca gcgtagacaa caggcagagc
     1921 agaagatgaa gagctggtgg caaggcaccc ccttgacagt gttccccttc gcgactatac
     1981 ttaacttata gattaagtat agtcacatag ctatgtggat acagaaagga acaacgatgc
     2041 cagccattat caaagaactg accatagaag ccccctcgca gcgcgtcttc agcgctctga
     2101 ctcagcctga cgaaataggt cactggtgga caaacgatct gaatgtcaca ccagaggtgg
     2161 gttcttttgc tgagtttcgc tttggggagt ggggggattt tgtcctcc
//
LOCUS       DASZAK010000349         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_470215, whole genome shotgun sequence.
ACCESSION   DASZAK010000349 DASZAK010000000
VERSION     DASZAK010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470215"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VGT44_06120"
     CDS             complement(<1..388)
                     /locus_tag="VGT44_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580412.1"
                     /translation="MLAALGSYVVEAWNDSTGFFSSLCSPMFKDQLTGFGFSNNGGKS
                     FTDMGGLPNVNCSTSAFEGDPSVEAYTSGGTTYFYISSLFFDSTNLAEDIAMDACVVA
                     GSGKSATLNCNPTPVIVANSGFFGFDD"
     gene            807..2075
                     /locus_tag="VGT44_06125"
     CDS             807..2075
                     /locus_tag="VGT44_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2580413.1"
                     /translation="MSLLETAERELIEAHQLARLHEGLQRVVPHNHFYTRKLLGKHST
                     ISLESLEQFSLLSFTTKQELVADQEAHSPFGTNLTYALSDYIRLHQTSGTTGSPYKVL
                     DTQESWDWWADCWSSVYSAAGVSRDDIVFLAFSFGPFIGFWSAYEGAKRFGALTVPGG
                     GMDSVQRLQAIQDSGATVLVSTPSYALRLAEVAQEQGIDLRALNVRITIHAGEPGASI
                     PATRQRIEQAWGAKTYDHAGMTEMGAYGFTCEEQQGLHVNESEFIAEILDPRHHRPVG
                     VGETGELVLTNLGRWGSPAIRYRTGDLVRHGGYQCACGRSYLFLPGGVLGRADDMLIV
                     RGVNIYPSALANILHRFPEVQEYRLIVTSEGALDEIALQVECPPSLIPAIQQALHIAF
                     NLRVPVEAVPPGTLPRFELKARRVDDRRNK"
     gene            2406..>2806
                     /locus_tag="VGT44_06130"
     CDS             2406..>2806
                     /locus_tag="VGT44_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580414.1"
                     /translation="MMCGIGRNGRFGRDTYLWSPTEKLNRKLWYAMLLCLLLLLAGCG
                     AQAGQIVTDQALTSDNLNVQQHITPTVSISIPQQAISPTPASSSGGILPKPGSPPTNI
                     PGSGIGPAPYGPPPTLTTQEIQLTQQLFALI"
BASE COUNT          660 a          778 c          753 g          615 t
ORIGIN      
        1 tgtcgtcgaa gccaaagaag cctgaattcg cgacgataac cggcgtcggg ttgcagttca
       61 gcgtcgccga cttgccgcta cctgcgacaa cgcaggcatc catggcgatg tcctctgcga
      121 gattggtact atcaaagaac aggctgctga tgtagaaata ggtcgttccg ccagaagtat
      181 aggcctcgac gctcggatca ccctcgaacg cagaggtaga acagttaacg ttgggcaatc
      241 cacccatatc ggtgaaggac ttaccgccgt tgttcgagaa accgaagccg gtcagctggt
      301 ccttgaacat cggcgaacag agagaggaga agaagccggt cgagtcgttc catgcctcta
      361 cgacatagga gccaagagcg gccagcattc cccttgctgg cgcaagagac gcgaccccat
      421 tctttcgtga tttttcaagc tagtatcaat tcacgtaaga atcgggtttg caacgaaaag
      481 gactggtaac acgtagaaag cacatgggag cgtaattagc agtatcctac ccccttattg
      541 cacaagagta ctcttattat tgagagctgt caagtatata taattaatta tcttgacatt
      601 ttatgatata taatggaata atttgatacg atatgagacg aagcaatcag gcaaataatt
      661 tgaaaattgt gaaattttac ctattctgct atctattcga cgactcgcca actttacata
      721 ggacttatgc gcaatgatgt acaatgaaat aacattgcac atttgcttac cccaaagggc
      781 aaagacaatt tgcaggaggt cctggtatgt ctttattaga aacggctgaa cgcgagttga
      841 tcgaagcgca ccagctggcg cgcctacacg agggattgca acgcgttgtg ccgcacaacc
      901 atttttatac acgcaagcta ctcggcaagc attccacaat cagcctggaa agcctggagc
      961 aattctcgct tctatcgttt acgacgaagc aggagctggt ggccgaccag gaggcgcatt
     1021 ccccctttgg cacgaacctg acgtatgcgc tcagcgacta tattcgtctt catcaaacct
     1081 ccggcactac ggggtcgcct tataaggtac tcgacacgca ggaaagttgg gattggtggg
     1141 ccgactgctg gtcgagtgtg tatagcgcgg cgggcgtcag ccgggatgat atcgtcttcc
     1201 tggccttcag tttcggcccg ttcatcggct tctggtcagc atacgagggc gctaaacggt
     1261 tcggcgcgct gaccgtcccc ggcggcggca tggactctgt gcagcgactg caagccatcc
     1321 aggatagcgg cgcgaccgtg ctggtctcca cgcctagcta tgccttgcgc ctggcggagg
     1381 tagcgcagga acagggcatc gacttgcgtg cgttgaatgt gcgtatcaca atccacgccg
     1441 gggagccggg ggcgtcgatc cccgcgacgc gccagcgcat cgagcaagcc tggggcgcaa
     1501 aaacctacga ccacgcgggc atgaccgaga tgggcgcata cgggttcacc tgcgaagaac
     1561 agcaggggct gcacgtcaac gagagcgaat tcatcgcgga aattctcgat ccgcgccatc
     1621 accgcccggt aggtgtgggc gagacaggcg agctggtgct gaccaatctg ggacgctggg
     1681 gaagccctgc tattcgctat cgcacgggag acctggtgcg gcatggcggc tatcaatgcg
     1741 cgtgtgggcg cagctatctc ttcctgccgg gaggagtgtt agggcgcgcc gacgatatgc
     1801 tgatcgtgcg cggcgtcaac atctacccct ccgcgctggc aaatatcctg caccgcttcc
     1861 ccgaagtaca ggaatatcgc ctgatcgtta ccagcgaagg cgcattggat gaaattgcgc
     1921 tgcaggtcga atgcccccct tcgcttattc ccgccattca gcaggccctg catatcgctt
     1981 tcaacctgcg cgttccggta gaagccgtcc cacccggcac attgcctcgc tttgaattga
     2041 aggccaggcg tgtcgatgac cggcgcaaca aatagtgttg aacgccgata caatcgacca
     2101 gccaaaagta ccacataatc catgcttgcg tcgcgttcat ttaacgtgta tgctacactc
     2161 ctacatgtgt tctattatca tacgcgccgg agagtagctg ttactatttt gcatggaagg
     2221 catgatttcc agggatgtgc ctccttccat agcacgatga agtttcacga ccaaagccgg
     2281 ggttgtcatc ctgagcgcag cgaaggatct cggtccccgg ccagtaagat ccttcgctgc
     2341 gctcaggatg acaacctgtt agcgatttgg ttttaaaact acatcatgcc tattaaggaa
     2401 atggaatgat gtgtggtatt ggtcgtaatg gtcgttttgg cagggataca tatttgtggt
     2461 cgcccactga aaagctgaac aggaagctct ggtatgcgat gctgctttgc ctgcttctgc
     2521 tgctcgccgg ttgcggagcg caggcaggcc agatcgtcac cgaccaggcg ctgaccagtg
     2581 ataacctgaa cgtccagcag cacattacac caacggtctc catcagcatc cctcaacaag
     2641 cgatctcgcc aactccagct tcctcgtctg gcggcatcct gcccaaacct ggctcgcccc
     2701 cgaccaacat ccccggcagc ggcattggtc ccgctcccta cggcccaccg ccaacactga
     2761 ccacgcaaga aatccaattg acgcagcagc tctttgcgct gatcaa
//
LOCUS       DASZAK010000350         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_577032, whole genome shotgun sequence.
ACCESSION   DASZAK010000350 DASZAK010000000
VERSION     DASZAK010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577032"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VGT44_06135"
     CDS             <1..548
                     /locus_tag="VGT44_06135"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV2580415.1"
                     /translation="NSTGQIAILSAASTAANQNTWIGFMKQELASKYPNMQLVKVAYG
                     NDDDQTSFNQTLALLQQYPNLKGIISPTTVGIAAAARALESVHKGGQVALTGLGTPNQ
                     LRQYVKDNTIKGFELWDPGNLGYLAYYVAALLVEGKLKGNVGETFTAGKLGSYTIAAT
                     NVVLLGPPTVFNSANIDQFNF"
     gene            638..952
                     /locus_tag="VGT44_06140"
     CDS             638..952
                     /locus_tag="VGT44_06140"
                     /inference="COORDINATES: protein motif:HMM:NF017174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEV2580416.1"
                     /translation="MQRIAFTMRIKPGTEAEYRRRHRQVWPELLADLKRAGCQNYSIY
                     LRGTELFAYMEVDDFQRYLEIMAASHASERWEAQMGDILFREIEEATGFPLVLEEVFH
                     LD"
     gene            1111..2325
                     /gene="rhaI"
                     /locus_tag="VGT44_06145"
     CDS             1111..2325
                     /gene="rhaI"
                     /locus_tag="VGT44_06145"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEV2580417.1"
                     /translation="MSSEIENRLQERDVTIESVIAAIQQFSIETPSWGYGNSGTRFKV
                     FHWPGAASNLREKLADAAQVQRVTGICPSVAIHNPWDRADDWHAVKVFAGQLGLQIGA
                     VNPNLFQDDAYRFGSLCHFDKQVRHQAIEHVIECIQIAQEVGSDVISLWLADGTNYPG
                     QDDLRRRRRRLLDSLQAIYAALPAGMRLLIEYKFFEPSFYSTDLPDWGTSLFMAQKLG
                     PRAEVLVDTGHHPQGTNIAQIVAQLLDEDRLGGFHFNDRRYADDDLIVGSQNPFELFC
                     IFNEIVGAQVEGNANAGRIAYMIDQSHNIEPKIEAMIQSVLNVQMAYTKALLVDHVTL
                     QQAQEAGDVLGAYRVLQAAYETDVRPFLSEARVRLGLDPDPIAAFRASGYAERVARER
                     IAAAMSSGYPGA"
     gene            2401..>2740
                     /locus_tag="VGT44_06150"
     CDS             2401..>2740
                     /locus_tag="VGT44_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional rhamnulose-1-phosphate
                     aldolase/short-chain dehydrogenase"
                     /protein_id="HEV2580418.1"
                     /translation="MTSFTTPTTLAQLVERSRKLGADRSIANWGGGNTSAKAIEVDFR
                     GRQVRILWVKGSGSDLATVTEASFTGLYLEDVLPLRERERMSDTEMVDYLAHCFYEPT
                     RPRPSIETLLH"
BASE COUNT          606 a          766 c          775 g          593 t
ORIGIN      
        1 ttaatagcac cggccagatt gccattctct cagcagctag cacggctgcc aaccagaaca
       61 cctggattgg cttcatgaag caggaactcg cgtcgaagta tccaaacatg cagttggtca
      121 aggtcgctta cgggaacgac gatgaccaga ccagctttaa ccagactctg gccctactgc
      181 agcagtatcc aaacctcaag ggaatcattt ctcccaccac ggtgggtatc gctgccgcgg
      241 ctcgcgcgct cgagtcggtg cataaaggcg gccaggtggc attgaccggc cttggtacgc
      301 cgaatcaact gcgacagtac gtcaaagata acacaatcaa gggcttcgag ctgtgggacc
      361 ccggcaacct tggttacctg gcctattatg tcgcggctct actggttgag ggcaagttga
      421 aagggaatgt cggcgagacg ttcactgctg gcaagctggg tagctataca attgccgcca
      481 cgaacgtggt cctacttggc ccacctacag tgttcaacag tgcaaacatc gaccagttca
      541 acttctaggt tggttcgcca tttttcgggg aggcatggag agtaggccgc ttctctgcgt
      601 gccctcctga gattggctta cggagcaggg gatttcaatg cagcgtatcg cttttacgat
      661 gaggatcaag cccggtaccg aagcagagta tcgccggcgt caccggcaag tctggccaga
      721 gctcctggca gacctcaagc gagcggggtg ccagaactac tccatctatc tgcgcggcac
      781 agagctgttc gcttatatgg aagtcgacga ttttcagcgc tacctggaga taatggcagc
      841 cagccatgct agcgaacgct gggaggctca gatgggagat attttattcc gcgaaatcga
      901 ggaggctacg gggtttccac ttgttcttga ggaagttttt catctcgatt aatataccgg
      961 tgtacgagat gtaaagccca tacgggcggc tttgagaact aacctcgctt gcgacccggg
     1021 ggcggagcat acgttcgttt gcggaaagtt aggcgaggaa acaaacctat cccgtcacaa
     1081 aaaccagcaa tatcaggcaa aggtgagtac gtgagttcag aaatcgaaaa tcgcttgcaa
     1141 gaacgagatg tcactatcga aagcgtcatc gcggctattc aacaattcag cattgagact
     1201 ccctcgtggg gatatggcaa ttccgggacg cgcttcaagg tctttcactg gcctggggca
     1261 gccagtaatc tgcgcgagaa actagcagac gcggcccagg ttcagcgtgt cactggcatc
     1321 tgtcccagtg ttgccattca caatccctgg gacagagcag acgattggca tgcagtgaag
     1381 gtgtttgctg ggcaactcgg cctgcaaatt ggtgccgtga atccgaacct ctttcaggac
     1441 gacgcgtacc gcttcggcag cctgtgccat ttcgacaagc aggtacgaca tcaagccatc
     1501 gagcatgtga tcgaatgcat ccagattgct caggaggtcg gctcggacgt tatcagcctg
     1561 tggctggctg atggcaccaa ttatcccgga caggatgatc tgcgccgccg ccgccgccgc
     1621 ctgctggatt ccttgcaggc catctacgcg gcattacccg ctggcatgcg cctgcttatc
     1681 gagtacaagt tcttcgagcc gtcgttttat tccaccgacc tcccggactg ggggacgtcg
     1741 ctattcatgg cccaaaaact tggtccgcgg gcagaggtgc tggtagatac cggacaccat
     1801 ccccagggaa cgaacatcgc gcagatcgtt gcgcagttgc tcgacgaaga ccgactggga
     1861 ggcttccatt tcaacgaccg caggtatgcc gacgatgacc tgatcgtcgg ttcccagaac
     1921 ccctttgaac ttttctgcat cttcaatgag atcgtcggcg cgcaggtcga gggcaacgcc
     1981 aatgctggcc gcatcgccta catgatcgac cagtcacaca atatcgagcc gaagatcgag
     2041 gctatgatcc agagcgttct caatgtccag atggcttata ctaaagcgct gctggtggac
     2101 cacgttacgc tacagcaggc gcaggaggct ggcgatgtgc tgggagcgta ccgcgtgctg
     2161 caggcagcgt atgagacaga tgtgcgccca tttctctccg aggcccgtgt ccgcctggga
     2221 cttgaccccg atcccatagc ggcattccgt gctagtggct acgctgagcg cgtagcacgc
     2281 gaacggattg ccgccgcaat gtcaagtggc tatccgggtg cgtagggacc cgcgttatcg
     2341 cgcctttcca cgtctgctcc ggcaaagcgg ttcttaattg atcacgaggg attgattgct
     2401 atgacaagct ttactacacc aactacactg gcgcaactcg ttgaacgctc gcgcaagctg
     2461 ggagcggacc gctctatcgc taactgggga ggtggcaaca cctcggccaa ggctatcgag
     2521 gtcgattttc gcggtcgcca ggtgcgtatc ctgtgggtca aaggctccgg ctccgatctt
     2581 gccaccgtta cagaagcctc ctttaccggg ctgtacctgg aagatgtgct tcccttgcgc
     2641 gaacgcgagc gcatgagcga taccgagatg gtggactacc tcgcgcactg cttctatgag
     2701 ccgactcgtc cgcgaccatc tatcgagacc cttttgcatg
//
LOCUS       DASZAK010000351         9154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_8864, whole genome shotgun sequence.
ACCESSION   DASZAK010000351 DASZAK010000000
VERSION     DASZAK010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9154
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8864"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VGT44_06155"
     CDS             <1..710
                     /locus_tag="VGT44_06155"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV2580419.1"
                     /translation="VLYAPLYSGDSQLPEVALTFDDGPNPFYTPQILAILQQYNVKAT
                     FFDVGYLVLDYPDIVRQEFKAGNIVANHSWSHPLLPSLSAQAVHDQLGRTSDAIQAVT
                     GNRPIFFRPPYGSYNSLVIAQAKSFGLSTIIWNDEAHDWMLPGVGVIVSRILSLARSG
                     AIILLHDGGGIRAQTVAALPYIITALRNRGYSFVTVEQMVRHLTGHINASLKGTIAHE
                     GAINHLIQEEIIYDRPH"
     gene            694..1410
                     /locus_tag="VGT44_06160"
     CDS             694..1410
                     /locus_tag="VGT44_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_263013192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:895..897,aa:Sec)
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV2580420.1"
                     /translation="MTDPIDPTGYNYTVFEGQGDNAAFPTPLYAGTVAPDCAAVLLES
                     GQEVRLSDYWRERDVLIEFGSYTUPYCSLAAPALEPMAKEFAEKGFTFLFLYTREAHP
                     GQQHGAHTSMEQKLAYAREFREDCRIERPILVDDLAGTAHRLYGDLPNMTYLIGRGGR
                     ILFRSNWTDAPTIEMMLNYLVNVRARRREGERLAPFYAEFAGYRITDPAKFQAGLHIG
                     GQQAIEDFARMMKIWAEQGR"
     gene            complement(1478..1702)
                     /locus_tag="VGT44_06165"
     CDS             complement(1478..1702)
                     /locus_tag="VGT44_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580421.1"
                     /translation="MNPQIQSEKTIETSLIPATISDEDTLALSGFSTDEIVSLLYLRQ
                     KYQTGGSDRVEVVRHLEFLKLMVMSGKIDL"
     gene            complement(2221..4689)
                     /gene="leuS"
                     /locus_tag="VGT44_06170"
     CDS             complement(2221..4689)
                     /gene="leuS"
                     /locus_tag="VGT44_06170"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV2580422.1"
                     /translation="MITRTKYNPKEIEHKWQTQWEAAGLFHAPDDSPKPRFYNLVMLP
                     YPSGDLHIGHWYNYTGTDTYGRYKKMLGYNVMQPMGFDAFGLPAENAAISRGVQARTW
                     TLSNIDNMRRQLRRMGAQWDWDREMVSCLPDYYKWTQWLFLEIYKHGLAYRTKAPANW
                     CPSCNTTLANEQVLANGTCERCGTMVIRKEIDQWLFRITNYAEELLDFSQIQWPEKTV
                     TMQRNWIGRSEGAEIRFYTQIDDQREEIPVFTTRPDTIYGATFFVMAPEHPWVEKITT
                     PEYRAEVEAYVDQARHMSEIERMSTEKEKTGVFTGGYVTNPVNGEQVPVWIADYVLMG
                     YGAGAIMGVPAHDERDFEFARKFGIEIREVIRPEGEEPSDPATWSEAKVALGVMVNSG
                     PFDGTPAEDAIAKITSYLEEQGIGKFMVSYRLRDWLISRQRYWGAPIPIIYCPEHGIV
                     PVPEDQLPVRLPENVQFKPTGESPLRYEPDFVNTTCPICGGPATRETDTMDTFMCSSW
                     YFLRYADPHNDQQAWSQEAMQRWLPVDQYIGGAEHAVMHLLYARFFIKALRDMGHLTF
                     DEPFTRLYHQGTVLGPDGQKMSKSRGNVVAPDAVVEKYGADTVRAYLMFMGPFDAGGS
                     FIAENLEGVWRFLNRFWSLVNDTWVERPTSEVTDESRAIERLRHKTIKRVTEEYANFR
                     FNTALAALMECNNVLVKQHREPVARSAAFRETLETMMQLLAPLTPHIAEELWQITNHH
                     GSVHQTSWPTYTEALTQDDTFTLVIQVNGKVRERIEVRSDISEQQARQVALENTRVAS
                     FIGESTIQKVIYVPGKLVNIVVRA"
     gene            complement(4797..4872)
                     /locus_tag="VGT44_06175"
     tRNA            complement(4797..4872)
                     /locus_tag="VGT44_06175"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4837..4839),aa:Ala,seq:ggc)
     gene            complement(5105..5177)
                     /locus_tag="VGT44_06180"
     tRNA            complement(5105..5177)
                     /locus_tag="VGT44_06180"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5142..5144),aa:Ala,seq:cgc)
     gene            complement(5248..6009)
                     /locus_tag="VGT44_06185"
     CDS             complement(5248..6009)
                     /locus_tag="VGT44_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580423.1"
                     /translation="MEVEAVVLKSLAKDSQERFESVQAFALALERAYLVGEEVTAMTQ
                     RSSSPLPIVAQENPPITISSQTAESKQLRLSRRAILLGLSGVAIAGGITYFSFADSKK
                     IKVTAAHSPPPVTHPLKATLVNTFKGHKYAISDLDWSPDGKLIASASYDESVKIWDAT
                     TGNHVVFFPYVDNYAFSAKFLPGSGARIASGSGKINVKPVGSVKIWDAARGTLLYTYT
                     GHTDRVTAIAWSPDSKYLASGGNDNDPTVHVWTLS"
     gene            complement(5984..6718)
                     /locus_tag="VGT44_06190"
     CDS             complement(5984..6718)
                     /locus_tag="VGT44_06190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2580424.1"
                     /translation="MSDRVGQVLGSYRLIRLLGEGSFAEVYFGEHIHLEIPAAIKILH
                     TQLTSEAIEAFRNEARMIARLEHPHIVRVFDYGVDDKTPFLVMNYAEQGSLRLRHPTG
                     TRLSLATIISYIKPLADALKYAHDQKLIHRDIKPENLLVGKHEEILLSDFGIAVMAQS
                     SRDLSNQGMTGTVTYMAPEQIEGKPRRASDQYSLGIIYSLRMAHWRGAIQGQLSGNCR
                     PALINGSSLHMLAESSHTVGSRSSRS"
     gene            6889..7638
                     /locus_tag="VGT44_06195"
     CDS             6889..7638
                     /locus_tag="VGT44_06195"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV2580425.1"
                     /translation="MKATRRKEISRTKQRRRKCRPHLFLTLNYHIIDRTIQDSIAISE
                     EAFEAQMAYLREHGYRALSIQQAIDDIYGRRSAPPRSLLLTFDDGYADNVRTALPILQ
                     QYGMRATLFVISAYVGQRNRWNPRACYDTLHMTWDDLRFWLAGGCDFGGHSHQHLCMT
                     RLDEEELLASVQVNKRLLEEQLHISPRAFAYPYGRFNQAVQEVVRQHYEVAFAVERGG
                     WNAPADRYAINRVMVLPTWSLAEFGEQLSHR"
     gene            complement(7625..7915)
                     /locus_tag="VGT44_06200"
     CDS             complement(7625..7915)
                     /locus_tag="VGT44_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580426.1"
                     /translation="MAIVVMQDFPGGTQEQYDNVTAQLNLGGHSPRGNLVHTAGPIEG
                     SWRVVDVWESQDALDAFMGVLGPIAHSAGIPQPQVTMWPLHNMLTPGYAIGG"
     gene            complement(8031..8885)
                     /locus_tag="VGT44_06205"
     CDS             complement(8031..8885)
                     /locus_tag="VGT44_06205"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEV2580427.1"
                     /translation="MIKLYLNDKQLTAIPDYALNQADLEELHFYKNQLTDLPASLWQL
                     TNLRFLNISANRLTSLSEGIGNLTRLHTLDLGHNQLTALPDALGNLTNLTNFLYLSNN
                     HLTSLPATLTRLSNLRYLNVTDNRLTALPEDIGNMQSLIELRAYNNQLATLPESLGNL
                     ANLKELHLMRNKLTSLPVSLGRLTGLRELRLEGNLLATLPESIGNLTRLRILDLRNNE
                     LTTLPDAPDNLASLTHLDLRANRLTTLPASIGNLPNLEKLDLRWNKLAERPSWLQQLE
                     KRGCIVYL"
BASE COUNT         1911 a         2406 c         2676 g         2161 t
ORIGIN      
        1 ctgtactgta tgctccgctg tacagcggcg atagccagtt gccggaagtc gcgttgactt
       61 tcgacgatgg gccaaatcct ttttatacgc ctcagatcct ggctatcctg caacaatata
      121 acgtcaaggc gaccttcttc gatgttggct acctggtcct agactacccg gatatcgtgc
      181 gccaggagtt caaagcggga aacatcgtgg cgaatcatag ctggtcacac cccttgctcc
      241 caagtctctc cgcacaggcg gtacacgacc agctggggcg aacctccgat gcgattcagg
      301 cggttacagg gaatcgcccg atcttcttcc gcccacccta cggttcttac aatagtctgg
      361 tgatcgccca ggcaaaaagt ttcggcctct caaccatcat ctggaatgac gaagcgcatg
      421 actggatgct accgggcgtc ggtgtcatcg tcagccggat actcagtctc gcgcgcagtg
      481 gcgctatcat tctcctgcat gacgggggtg gaatccgcgc gcagacggtc gcggcgctgc
      541 cgtatatcat cacggctcta cgcaatcgcg gctatagctt cgtcaccgtc gagcagatgg
      601 tacgccatct caccggacat atcaatgctt cgctgaaagg aactattgct cacgagggag
      661 ccatcaatca ccttattcag gaggaaataa tctatgaccg accccattga cccaaccggc
      721 tacaactaca ccgtgtttga aggccagggc gacaacgcgg ctttcccgac gccgctgtat
      781 gcggggactg ttgcgccgga ttgcgccgct gtcctgcttg agagcgggca ggaggtgcgg
      841 ctgagcgatt actggcggga gcgggatgtg ctgatcgagt ttgggtcgta tacctgaccg
      901 tactgttcgc tggcggcgcc agcgctcgaa ccgatggcga aagagttcgc cgagaagggc
      961 tttaccttcc tgtttctgta tacgcgcgag gcgcatccgg ggcagcagca tggcgcgcac
     1021 accagcatgg agcagaagct ggcctacgcg cgggagtttc gcgaagattg tcgcattgaa
     1081 cgaccgatcc tggtcgatga ccttgcaggc accgcccacc ggctatatgg cgatctaccc
     1141 aacatgacct atttgatcgg gcgaggcggc agaattctct tccgctcgaa ctggacggac
     1201 gcgcccacca tcgaaatgat gttgaactac ctggtgaatg tacgggcaag gcggcgcgag
     1261 ggcgaacggc tggcgccctt ctatgcggag ttcgcgggct atcgcatcac cgacccggct
     1321 aaattccagg cgggtttgca tattggggga cagcaggcta tcgaggattt cgcgcgcatg
     1381 atgaaaatat gggcggagca aggcagatag tacaactgaa ggcaggcagg atgactcatg
     1441 gcgcgagtcg ccctgcctgc ctttttcacg cctgttgtta caggtcgatt ttcccgctca
     1501 tgaccatcaa cttcagaaat tccaggtggc gcaccacttc gacgcggtca ctgccgccgg
     1561 tttggtattt ctggcgcagg tagagcaacg agacgatttc gtcggtgctg aagccgctca
     1621 gcgccagcgt gtcctcgtcc gaaatggttg ccggaatgag agaagtttca atagtttttt
     1681 cagattggat ttgtggattc attgttatag ggtcctttct ttttagaatc aggaattagt
     1741 aaggtatgct tccgctaaaa taactcgcta ctcagactct tgaatgtccg tccttcatct
     1801 tccactttct tattctttct gagctaagta taaacggaaa cgctgcctac tactatctac
     1861 gaaaatacac ctgggatgtg aaatacttca cgcttacggt gctgctgtac ggttctgcat
     1921 gagaagacgc gctatgaact cttatgatga actttctggc gactcactac aaacggttct
     1981 ttgataagat atacgtttga aagtgctaga atgttcaatg aaagtgtgcc aattttctgt
     2041 cggttgaaaa ttcacccata cgtcagaaat tcggctgaag gcgtttattc ttcccctcga
     2101 taatatagac gctggcgagt tacaaattat tgcatgagaa attgtaattc aatacacaag
     2161 cagacgaaag tacgtgagcc gtcggctcac gtactttcgt ctgcttgtgt attgaaacat
     2221 ctatgctcgg acgacgatat tgaccagttt gccggggacg tagatcacct tctggatggt
     2281 cgattcgccg atgaaggagg ccacgcgcgt gttttccagc gcgacctggc gcgcctgctg
     2341 ctcgctgatg tcggagcgca cctcaatgcg ctcgcgcacc ttgccattga cctggatgac
     2401 cagcgtaaag gtgtcgtctt gtgtcaacgc ctcggtgtag gtcggccacg aggtctggtg
     2461 gacactgccg tggtggttgg tgatttgcca cagctcttcg gcgatgtgcg gcgtcagggg
     2521 agccagcagc tgcatcatgg tctccagcgt ctcgcggaag gcggcactgc gcgccaccgg
     2581 ctcgcgatgc tgtttgacca gcacgttatt gcactccatc agagcggcca gggcggtgtt
     2641 gaagcggaag ttggcgtact cctccgtgac gcgcttgatg gttttgtggc gcaggcgttc
     2701 gatggcacgg ctttcgtccg tcacctcgct ggtggggcgt tcgacccagg tgtcgttgac
     2761 caggctccag aagcggttca ggaagcgcca cacgccttcc aggttttcgg cgatgaagct
     2821 gccgcccgcg tcgaagggtc ccatgaacat caggtaggcg cgcacggtat ctgcgccgta
     2881 cttctcgacc accgcgtcgg gggcgacgac gttgccgcgc gatttggaca tcttctgtcc
     2941 atccggcccc agcaccgtcc cctggtggta caggcgcgtg aacggctcgt caaaggtcag
     3001 gtggcccatg tcgcgcaaag ctttaatgaa gaagcgcgcg tagagcaggt gcatgacggc
     3061 gtgttccgcg ccgccgatgt actgatcgac gggcagccag cgctgcatag cctcctggct
     3121 ccaggcttgc tggtcgttgt gcgggtcggc gtagcgcagg aagtaccacg aggaacacat
     3181 gaaggtgtcc atcgtatcgg tttcgcgcgt ggcagggccg ccgcagatgg ggcaggtggt
     3241 attgacgaag tccggctcgt agcgcagcgg cgactcgccc gtcggcttga actgcacatt
     3301 ttcaggcagc cgcaccggca gctggtcttc aggcactggt acgatgccat gctccgggca
     3361 gtagatgatc gggatgggcg cgccccagta gcgctgtcgg ctaatcagcc agtcgcgcag
     3421 gcggtaggag accatgaact tgccgatgcc ctgttcttcc aggtagctcg ttatcttcgc
     3481 gatggcatcc tctgcgggcg ttccatcgaa cgggccggag ttgaccatca cgcccagggc
     3541 gaccttcgcc tcgctccacg tggcggggtc gctcggttct tcgccctctg gccggatcac
     3601 ctcgcggatc tcgatgccga acttgcgcgc gaactcgaag tcgcgttcgt cgtgcgccgg
     3661 gacgcccatg atcgcgcccg cgccgtagcc catcagcacg tagtcggcaa tccagacggg
     3721 aacctgctcg ccgttgacgg ggttggtgac atagccgccg gtgaagacgc ccgtcttctc
     3781 cttctcggta ctcatgcgct cgatctcgga catgtgccgc gcctggtcaa cataggcttc
     3841 cacctcggcg cgatactccg gcgtggtgat cttctcgacc cagggatgct ccggggccat
     3901 gacgaagaag gttgcgccat agatggtgtc ggggcgcgtc gtgaagacgg ggatttcttc
     3961 acgctggtcg tcgatctgcg tgtagaagcg gatctccgcg ccctcgctac gcccgatcca
     4021 gttgcgctgc atcgtgacgg ttttttctgg ccactggatt tgcgagaagt ccagcagttc
     4081 ttccgcgtag ttggtgatgc ggaacagcca ctggtcaatc tctttgcgaa tgaccattgt
     4141 gccgcagcgc tcgcacgtgc cgttcgccag cacctgctcg ttggccagcg tggtattgca
     4201 gctaggacac cagttggcgg gggctttggt gcgataggcc aggccgtgct tgtagatttc
     4261 caggaacagc cactgcgtcc acttatagta atcgggcagg caggagacca tctcgcggtc
     4321 ccagtcccac tgcgcgccca tgcgccgcag ctgcctgcgc atattatcga tgttggacag
     4381 cgtccaggtg cgcgcctgca cgccgcgcga gatggcggcg ttttcggcgg gcagaccgaa
     4441 ggcatcgaag cccatcggct gcatgacgtt ataacccagc attttcttgt agcggccata
     4501 ggtgtccgtg ccggtgtagt tataccagtg gccgatgtgc aggtcgccgg aggggtaggg
     4561 cagcatcacc aggttataaa agcggggttt gggcgagtcg tcgggagcat ggaagagacc
     4621 cgccgcttcc cactgcgtct gccatttgtg ttcgatttcc ttcggattgt actttgtgcg
     4681 tgtaatcatc ttctgctcct gaaactaaaa aagcctttca tcctcgcggg acgaaaggcc
     4741 atttcgtggt accacccttg ttcaacgctc gctctcggtt ctggcgaaaa agtggttggt
     4801 ggacctgagg gggctcgaac ccctgacctc atccatgcca tggatgcgct cttccagctg
     4861 cgctacaggc ccaggctgta ctttaaatga ttccgagaat gagactatac gtactcaaca
     4921 cgcgataacg ggcgcaccgg caaacgctag gcggccgtgt cgaggcttgt tcaagcctcg
     4981 cgagccattc acgtctgctg ctcgcaggcg agttcggtca actcgttgca gccttgcacc
     5041 acccggctgt tctctgtagg atttatgacc tactactcct gctcgtcgcg aaaggtctga
     5101 ccggtggaga tgagggggct cgaacccctg acctcgacag tgcgattgtc gcgctctccc
     5161 agctgagcta catccccagg caggaactat catagcatat gatggtggtg ttgtaaatag
     5221 gttatttccg ggtaatttcg gtcttgttca agagagggtc cagacatgta ctgttggatc
     5281 attatcgttg cctccggagg ccaggtattt actgtcgggg gaccacgcta tggcagttac
     5341 tctatcagtg tggccggtgt aagtataaag cagcgtaccc ctcgcagcat cccatatttt
     5401 gacactgcct acaggcttca catttatctt gccgctacca gaggcaatgc gcgcgccgct
     5461 acccggtaaa aattttgcag aaaatgcata attgtctaca tagggaaaga aaacaacgtg
     5521 gtttcctgta gtagcgtccc atattttcac tgattcatcg tagcttgctg aagcgatgag
     5581 tttaccgtcg ggagaccaat ccagatcact gatggcatat ttatgtccct taaaggtatt
     5641 aacgagtgtt gccttcaagg gatgtgtgac aggaggagga ctgtgcgcgg ctgtgacttt
     5701 tatctttttg gaatcggcaa acgagaaata ggtgatgcca cctgctatgg ccacgccgga
     5761 aagacccagg agaatagctc gccgggagag acgaagttgt ttcgattcag cagtttgaga
     5821 agagatcgta attggtggat tctcctgtgc aacgattggg agaggcgaag atgacctttg
     5881 ggtcattgca gtcacttctt ctccgacaag gtaagctcgt tccagggcga gggcgaacgc
     5941 ctgtacactc tcaaagcgtt cttgcgaatc tttcgcaagc gatttaagaa cgactgcttc
     6001 gacttccaac ggtatggctg gattctgcca gcatatggag ggaggaaccg ttgataagtg
     6061 ctggacggca atttccggat aattgccctt gaatggcacc tcgccagtga gccattcgta
     6121 gactatatat aatacccagg gagtattgat cgctggccct gcgcggcttg ccctcgattt
     6181 gttcgggtgc catgtaggtg acagtaccgg tcatgccctg attgctgagg tcacgcgaac
     6241 tttgcgccat cacggcgatg ccaaaatcgc ttaacaggat ttcctcgtgt ttccccacca
     6301 gaaggttctc tggtttgatg tcgcggtgga ttagcttttg atcgtgagca tacttgagcg
     6361 cgtcggccaa tggcttgata tatgagatga tggttgcgag cgacaggcga gttcccgtgg
     6421 gatggcgcag gcgcaggctg ccttgctcgg catagttcat taccagaaaa ggggttttgt
     6481 cgtcaacgcc gtaatcgaag acccgcacaa tatggggatg ttctaatcgt gcaatcatgc
     6541 gagcctcgtt gcggaatgct tctatcgcct cgctggtcaa ctgtgtgtgc agaattttga
     6601 tggcagcggg gatttccaaa tggatgtgtt cgccgaaata gacttcggca aagctgcctt
     6661 ctcccaaaag gcgaataagc cgataactac ccaatacctg gcctacccgg tcagacatac
     6721 tcgtaccccc agtctgatag atatgtagcg gccaaaagtg gagaaatgat gtttaaatgg
     6781 gaaaattgta actcgattat aacatataga cgatatcatt gcgcctccag cacgccatct
     6841 gataggatgc agctgtaaag cgcgggagca atagcttgat taggaaagat gaaagcaacg
     6901 aggaggaagg aaatctcgcg cacgaagcag agaagaagaa agtgtaggcc gcacctgttt
     6961 ctaacattaa attaccatat catcgaccgg accattcagg acagcattgc tatttcggaa
     7021 gaagcattcg aggcgcagat ggcctatttg cgcgagcatg gctatcgtgc gctttcgata
     7081 cagcaggcca ttgatgatat ttatgggagg cggtcagcgc cgccgcgctc gctgctgctg
     7141 acctttgatg atggttatgc cgataatgtg aggacggcgc tgccgatctt gcagcaatac
     7201 ggcatgaggg ctacgctgtt cgtcatttcg gcctatgtcg gacagcgcaa tcgctggaat
     7261 ccgagagcct gctatgacac gctgcatatg acctgggatg atctgcgctt ctggctggct
     7321 ggcggctgcg attttggagg tcacagccac cagcatctct gtatgacgcg cctggatgaa
     7381 gaggaattgc tggcgagcgt ccaggtcaat aaacgcctgc tggaagagca attacatatc
     7441 agcccgcgcg ccttcgccta tccctatggc cgctttaacc aggcggtaca ggaagtagtg
     7501 cgccagcatt acgaggtcgc ctttgccgtc gagcgcggcg gctggaacgc gccggccgac
     7561 cgctatgcca tcaaccgggt gatggtcttg cctacgtgga gcctcgcgga atttggcgag
     7621 cagcttagcc accgatagcg tagcctgggg tgagcatgtt gtgcagcggc cacattgtca
     7681 cctgtggttg aggaatcccc gcgctgtgcg cgattggccc cagcacaccc atgaaggcat
     7741 cgagcgcatc ttgcgactcc cagacatcaa cgacacgcca gctgccctct atcggccccg
     7801 ctgtgtgaac aaggttgccc ctggggctgt gaccgcccag attgagctgc gctgtgacgt
     7861 tatcgtactg ctcctgcgta ccgccgggaa agtcctgcat tactacgatt gccatgtgat
     7921 tgttcctcct ttgtaatatc tgaacaaatt cagcttttgt tctcaggagt aactataccg
     7981 gagaatggtg agcatgtaga tagcaaatgc gtatcacgga ggtatcattg ttacagatag
     8041 acgatacagc ctcgcttctc aagctgttgc agccacgagg ggcgttcagc cagcttgttc
     8101 cagcgcaggt ctagtttttc gagattagga aggttgccga tacttgccgg gagggtggtc
     8161 aacctgttcg ctcgcaaatc gagatgggta agactcgcca ggttgtcagg cgcatcgggg
     8221 agcgtggtaa gctcgttatt tcgcagatcg agtattctga ggcgtgtcag gttgccgatg
     8281 gactcaggga gtgttgctag caggttgccc tccagtcgca gttctcggag gccagttagc
     8341 cggccgaggg atacggggag agatgtcagc ttgttgcgca tcaggtgcaa ttccttgagg
     8401 tttgccaggt tgccgagcga ctcaggcagc gttgccagtt gattgttgta cgcccgcagt
     8461 tcgatgagac tctgcatgtt gccgatatct tcggggagag ccgtcaaacg attatcggtg
     8521 acatttagat agcgaaggtt actcagcctg gtcagcgtgg ctggaagaga ggtcaggtgg
     8581 ttgttgctca ggtagagaaa attggtcagg ttcgtcaggt tgccaagcgc atcgggaagg
     8641 gctgttagct ggttatgacc gagatcgagc gtgtgaagtc tggtcagatt gccgatgcct
     8701 tctgacagag aggtcagccg gttggcggat atattcaaga agcgcaggtt tgtgagttgc
     8761 caaagcgacg cagggaggtc ggtcagttga ttcttgtaaa aatggagttc ctcgaggtcg
     8821 gcctggttta gagcgtagtc gggaattgcg gtcagttgct tatcgttcag gtacaatttt
     8881 atcaatatta ttctttccct ccggttgctg cttgtaaatt acaagtttcc gtgtcgtcgt
     8941 ctcgtgagaa gtagctgttg taatgagata gcgctcttcc ggctcatagc cagcgctttc
     9001 ggcggaaaag aacacctgtt ttacgacaaa caggtatcac gaaacgacga cacggaatat
     9061 ctggtcattt acaatcagcc aacgggagat gcctggtcca tgatcggatc atatccaggc
     9121 gacgaggacg cggtaactgc ccggagcgtg cggg
//
LOCUS       DASZAK010000352         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_435490, whole genome shotgun sequence.
ACCESSION   DASZAK010000352 DASZAK010000000
VERSION     DASZAK010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435490"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VGT44_06210"
     CDS             complement(<1..841)
                     /locus_tag="VGT44_06210"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580428.1"
                     /translation="MAERKPIGVISIGSNDIHLLVATCDGVAAFERQVNQSMLAELVG
                     TVNGGVVPVKALNKALQDLDELVMAARAAGTSTIIAIATEAMREVANGPVFIDLVGRT
                     LDIESVLISGQEEAGLDYCWATFPPVPQTPLLVVDSGGGSTQAIQGEGATPAFAKSLP
                     VGAGNMTKQFLAHDPPTIEEIQALNDHISVLVNTLPVVLPPHSAILMGGSADHLLQFT
                     TDPTKHLMTRDDLHHALGLLLKKPAKDIVRDYDMQVERARLLPAGAVILTNVLVRYGL
                     DEAH"
     gene            1076..2326
                     /locus_tag="VGT44_06215"
     CDS             1076..2326
                     /locus_tag="VGT44_06215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2580429.1"
                     /translation="MNAKTPDGDLKQAQRQALQQRIPWWSDIGPAVTAELQLPALHEQ
                     ALHGFDVVVIGAGVAGLSAALAIKQRDASLRVLVLEKEEMLGLGATGRNAGIFTPGVN
                     MAFSEMEPGDPARSFYPETTTLFHRLIAEAQEPGALLSAQQTGAINMATSKRAAQKLA
                     WETEERNAAGLRAELWSPAQVAGATQGRLNTRPVVNAMWLPDEGRIHPLTLLAHLARR
                     ARQFFGVTIVGQALVTTCEPTTDRTSPARWRLSLANGKDGMHGHDTIQTHAVVNCAGP
                     TDGANARIYALAFAADFPDDFPLFWDASPYTYADYRPGNGRLGVSGGRYGKAGVTTND
                     ASYYRRLADATRGWVPELAYQQPAYTWAVDLYVTSGLLPHLRLMDAGGRSAPGVAIEG
                     LGAHGVLPSMVLARQAADYLVKRL"
BASE COUNT          466 a          700 c          722 g          497 t
ORIGIN      
        1 catgtgcctc gtccagtcca tagcggacca ggacattcgt aagaataacg gctccggcag
       61 gaagcagacg cgcgcgctcc acctgcatat catagtcacg cacgatgtct ttcgcgggtt
      121 tctttaacag gaggcccaga gcatgatgca agtcatcgcg cgtcatgaga tgtttcgttg
      181 gatctgtcgt gaactggagc aggtgatcgg cgctgccacc catcaggatg gcactgtgtg
      241 gcggaagcac gacgggtagg gtgttcacga gaacagagat gtgatcgttc agagcctgta
      301 tctcttcgat ggtgggagga tcatgtgcca ggaattgttt ggtcatattg ccagcgccaa
      361 ccggcagcga tttggcaaaa gcgggcgttg ccccttcgcc ctggattgcc tgggtactcc
      421 cgcctccact atccacaaca agcaaaggtg tctgtggcac cggtggaaac gttgcccagc
      481 agtaatccag cccggcttcc tcctggcctg aaataagcac agattcgatg tccagcgtgc
      541 gaccaaccag gtcaatgaag accgggccgt tggcgacctc gcgcattgcc tccgtagcga
      601 tagcgataat ggtcgaggtg cctgctgcgc gcgctgccat caccagctca tccagatctt
      661 gtaaagcttt gttgagcgct ttgacgggaa ccactccacc gttcaccgtg ccgaccagtt
      721 ccgccagcat ggattgattc acctgccttt caaaggcggc gacgccatcg caggtggcaa
      781 ctagcaaatg aatatcattg ctgccaatgc tgatcacacc aattggtttt ctctcggcca
      841 tgatgttctc ctttgtaccg tgcttgggaa gtggcgaggt tgttttttct ccctgtattc
      901 tatctgatct gctcccatgc aacaatagag gtgggttgaa ttgagcgtat gttattatgt
      961 agacagagct aaccggcccg accagcaaac gttaccgggc gcgggtgaaa gccaggagtc
     1021 caggcaagta tatgatttgt tcaggttttt tgtaactatg caatctcgta catcattgaa
     1081 cgcaaaaact cccgatggtg acttgaaaca ggcgcagaga caagcgctcc aacagcgtat
     1141 cccctggtgg agcgatattg gacccgctgt gaccgctgaa ttgcaattgc ctgccttgca
     1201 tgagcaggcg ctgcatggat ttgatgtggt cgtcatcggc gctggcgtgg ccgggctgag
     1261 cgccgcgctc gccatcaagc agcgcgatgc ctcgttgcgc gtgcttgtgc tggaaaaaga
     1321 ggagatgttg gggctgggcg cgacgggccg caacgccggg atatttacac cgggtgtcaa
     1381 catggccttc tccgaaatgg aaccgggcga tccggcgcgc tcgttttacc ccgaaacgac
     1441 cacgcttttt caccgcctga tcgccgaggc gcaggaacca ggggcgctgc tctccgccca
     1501 acagaccggc gctatcaata tggcgacgag caagcgcgca gcgcagaagc tcgcatggga
     1561 gaccgaggag cgtaacgcgg ctggtctgcg cgcggagctg tggtcgcccg cgcaagttgc
     1621 cggggcgacg caagggcggc tcaatacgcg cccggtcgtc aacgctatgt ggctgccgga
     1681 tgaagggcgc attcacccgc tgactttgct ggctcacctc gcccggcgcg cgcgccagtt
     1741 tttcggcgtc acgatcgtgg ggcaggcgct tgtcactacc tgcgaaccca caactgaccg
     1801 tacatctccg gcgcgctggc gactgagcct ggcgaacggc aaagatggaa tgcatggtca
     1861 cgataccatc cagacgcacg ccgtcgtcaa ttgtgccggg ccgaccgatg gagccaacgc
     1921 gcgcatttat gccctggcct tcgctgccga cttccccgac gatttcccat tgttctggga
     1981 cgcctcgccc tatacctacg ccgattaccg gccaggcaat ggccgcctgg gcgtcagcgg
     2041 tggtcgctat ggcaaggcgg gcgtgacgac caacgacgcc tcctactatc gccgcctggc
     2101 cgatgccacg cgcggctggg tacccgaact ggcctaccag caaccggcct atacctgggc
     2161 tgttgatttg tacgttacct ccggcctgct tcctcacctg cgtctcatgg atgcaggcgg
     2221 aaggagcgcg cccggcgttg ccatcgaagg tctcggcgcg cacggtgtgc tgcccagcat
     2281 ggtgctagcc cgccaggccg ccgactatct cgtaaagcga ctgtaggggc gggggaggat
     2341 gtgaggcgga gtggggacgc ttgcgtcgcc cgttggttat gcgat
//
LOCUS       DASZAK010000353         1743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_683801, whole genome shotgun sequence.
ACCESSION   DASZAK010000353 DASZAK010000000
VERSION     DASZAK010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1743
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683801"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1464
                     /gene="lpdA"
                     /locus_tag="VGT44_06220"
     CDS             25..1464
                     /gene="lpdA"
                     /locus_tag="VGT44_06220"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV2580430.1"
                     /translation="MASTQYDVAVIGAGPGGYVAAIRAAQLGAKTAIVEKQYLGGTCL
                     NVGCIPSKAMLHIAEVMQSMASMGELGIELSQPPRFDMSKAVAFTANVVKRMTGGVGT
                     LMKGNHIDVFDGLGTVEASRTVTVSKSESAKSDGSLQQFSADKIILATGSVPLMPPFP
                     GIDGRNVINSDTCWHLPKLPESIICVGGGVIGVELACMFNGLGSQVTIVEMLSSILAP
                     VDDEVRNLLVRSLSKRGITIATNARVESIADEGNLKKVTASTPQGEKSFSGEYVLMAI
                     SRRANTSGLEQLMEQGLENDRGRVRVNEKMETNLPGIYAIGDLVHGAGLAHVASEEGE
                     VAADNAMGHAAKMDYNVVPNPIYTFPEIAFVGLTEAQAKEHYSDVRVERFPWAANGKA
                     VGSAHTEGFTKVILGKYNEILGAHIIGPDATNLITEYSVAMRNELTADEIIATIHPHP
                     TLSEGLREAVLAAEGRPIHIPPKQAARAR"
BASE COUNT          401 a          465 c          526 g          351 t
ORIGIN      
        1 cttagcacgc agaggagaaa gagcatggca agtacgcagt atgatgtcgc ggtgattggc
       61 gcgggccctg gtggctatgt tgctgccatt cgcgctgcac aacttggcgc aaagactgcc
      121 atcgtcgaaa agcagtatct gggcggcacc tgcctcaacg tcgggtgcat cccctctaag
      181 gcgatgctgc acatcgccga agtaatgcag agtatggcct cgatgggcga actgggcatc
      241 gaactctcgc agccacccag gtttgacatg agcaaagccg ttgcatttac cgccaacgtc
      301 gtcaagcgca tgactggcgg tgtcgggaca ctgatgaagg gcaaccatat tgatgtcttt
      361 gatgggctag gaaccgtcga ggcctcgcgc accgttactg tcagcaagag tgagagtgcc
      421 aagagtgacg gttcactgca acaatttagt gcggacaaga tcattcttgc taccggttct
      481 gtgccactca tgcctccatt tcccggcatt gatggtcgta atgtgatcaa tagcgacacg
      541 tgctggcacc tgccaaagct gccagaaagt atcatctgtg tcggtggtgg cgtaatcgga
      601 gtcgaactag cgtgcatgtt caatggctta ggctcgcagg tcacgattgt cgaaatgctg
      661 tccagcattc ttgctccagt ggatgacgag gtacgcaatc tccttgtgcg ttcgctcagc
      721 aagcgcggca tcaccattgc cacgaatgcc agggtggagt ccattgccga tgagggcaac
      781 ctgaagaagg tgacagccag cacaccacaa ggggaaaaga gcttcagcgg ggagtacgtg
      841 ctgatggcga taagtcggcg tgccaacacg agcggactcg agcagctcat ggagcagggg
      901 ctcgagaacg accgtggccg cgtgcgcgtc aacgagaaga tggagacgaa cctgcctggc
      961 atctatgcca tcggcgacct ggtgcatgga gctgggctgg ctcacgttgc ctcggaggaa
     1021 ggtgaagtgg cggctgataa cgctatgggc catgcagcga agatggatta caacgttgtc
     1081 cccaacccca tctatacctt cccggagatt gcgtttgtcg gcctgacgga ggcccaggcc
     1141 aaagagcact attccgacgt gcgggtcgag cgcttcccgt gggcagcgaa tggcaaggcc
     1201 gtcggcagtg cccataccga aggcttcacc aaagtcatcc tgggcaagta caacgagatt
     1261 ttgggtgcgc atatcatcgg cccagatgcg accaacctga ttacggaata ttcggtcgct
     1321 atgcggaatg agctcacagc tgatgaaatt attgcgacga tccacccgca tccaacactc
     1381 agtgaggggc tgcgcgaggc ggtcctggca gcggagggtc gtccaatcca tattccgccc
     1441 aagcaggctg cacgagctag atagtacacg tatttaagag cgcttctcca aaagaagtgt
     1501 tctccagatt tcgagggcct ccaggccctc tagcagtgag agaacaagga caccctgcca
     1561 gaggaggtgg ctgatatgaa ggacatacca gatacagaga cgaagactac caatgagcgt
     1621 ctgatcacac cacagagcgt tgagcctgcc ggtgaatctg ttcctgtggt catgccccgt
     1681 gtcgtgatcg tcggggctgg ctttggcggc ttacaggcgg cacgcgcctt acgtgatgcg
     1741 cct
//
LOCUS       DASZAK010000354         2607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_364444, whole genome shotgun sequence.
ACCESSION   DASZAK010000354 DASZAK010000000
VERSION     DASZAK010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364444"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1542
                     /gene="glnA"
                     /locus_tag="VGT44_06225"
     CDS             118..1542
                     /gene="glnA"
                     /locus_tag="VGT44_06225"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV2580431.1"
                     /translation="MTPEEVLQFATEQGAKMVDLKFADVPGTWQHFTVPLHEFEVDTF
                     VEGKAFDGSSIRGFQTINESDMLVIPDPDSAIMDPFTAVPTLSLVCNIAHPITRKPYT
                     RDPRHIAQKAEEFLRSTGIADTSYFGPEAEFFIFDNVRYNSSTNIQYAEVDSEEALWN
                     ASRNGGSPNLGHRMRVKEGYFPVAPNDTFMDLRTAMVLTLEACGVPVEMQHHEVGGPG
                     QAEIDMRFNTLTKTADNLLLYKYIVKNMAKKYGKTVTFMPKPVFGDNGSGMHVHQSLW
                     KNEQPLFFDKEGYASLSQLARYYVGGLLTHAPALLAIVAGTTNSYKRLVPGYEAPVNL
                     VFSKGNRSAAVRIPITDCAKAKRVEFRPPDPTANPYLAFAALLMAGLDGIQRGIEPSE
                     HGFGPVDRNLYHMSKAELAEIRSVPASLDAALDELEDDHAFLLEGGVFTSDLLEHYVE
                     FKRDQSAEVRLRPVPIEFELYYDV"
     gene            1969..2310
                     /locus_tag="VGT44_06230"
     CDS             1969..2310
                     /locus_tag="VGT44_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580432.1"
                     /translation="MIDGSLIPTYDELAAFIADKFATLRKPYMEWANLARRAVQGQPY
                     DMKQLATLERFINEQRAELRTILMIASEHLDEAQLALLRAQACMSKNAWKSLKKRELV
                     NLKDGFRLISY"
BASE COUNT          638 a          761 c          625 g          583 t
ORIGIN      
        1 ggtcaaacgc ccctggtcct tgcgcaattt tcaaaaacgt gatgaattgg atcagttaac
       61 cttcattctg caatggggcc gaaatgcaag gagaagaaga cgagaggtaa ggcgcacatg
      121 actccagaag aagtattgca atttgcgacc gagcaaggcg caaaaatggt ggatctcaag
      181 ttcgcggatg tccccggtac gtggcaacat ttcaccgttc ccctgcacga atttgaggta
      241 gacacattcg tcgaagggaa ggcgttcgat ggctccagca ttcgtggttt ccagaccatt
      301 aacgaaagcg acatgctggt gatccccgat cccgattcgg cgattatgga ccccttcacg
      361 gctgttccca ctttgagcct ggtctgtaac atcgctcatc ctatcaccag gaaaccctat
      421 acgcgcgatc cccgtcacat cgcgcaaaaa gcagaggagt ttctccgctc cacaggtatc
      481 gcagacacca gctatttcgg ccccgaagcc gaattcttca tttttgacaa cgttcggtat
      541 aacagcagca ccaacatcca atacgcggaa gtcgattcgg aagaagccct ttggaacgct
      601 tcgcgcaacg gcggttcgcc caatctcggc caccgcatgc gcgtcaaaga aggctacttc
      661 ccggtcgcgc ccaacgacac cttcatggac ctgcgcacag cgatggtttt gacgctggaa
      721 gcatgcggcg tgccggtaga gatgcagcat cacgaggtag gcggccctgg ccaggccgaa
      781 atcgatatgc gtttcaacac gctgacgaaa actgccgaca acctgctgct atacaagtac
      841 atcgtcaaaa atatggcgaa aaaatacggc aagaccgtca catttatgcc caaacccgtc
      901 tttggtgaca acggctccgg gatgcacgtg catcaaagcc tgtggaaaaa cgagcagccg
      961 ctgttctttg ataaagaagg atacgccagt ctttcccaac tggcgcgcta ctatgtcggc
     1021 ggtctgctca cccatgcccc ggcgctgcta gccatcgttg ccggcacgac aaacagttac
     1081 aagcgcctcg ttcctggcta cgaagccccg gtcaacctgg tcttctcgaa aggcaaccgc
     1141 agcgcagccg tgcgcattcc cattactgac tgcgccaaag cgaaacgcgt cgaattccgc
     1201 ccgcccgatc cgacagccaa cccctacctg gccttcgccg ccctgctcat ggccggcctg
     1261 gacggtatcc agcgcggcat cgagccgagc gagcatggct tcggcccggt tgatcgcaac
     1321 ctctatcaca tgtcgaaggc agaactcgcc gaaattcgct ccgtgccagc ctctctggat
     1381 gccgccctgg acgaactcga agatgaccat gccttcctgc tcgaaggcgg cgttttcacc
     1441 agcgacctgc tcgagcatta tgtcgaattc aaacgcgacc agtccgccga ggtgcgcctg
     1501 cgccccgtcc cgattgagtt tgaactgtac tacgacgtgt agccctcgta ggctcccatc
     1561 gtaagatgcg atgatgaagt ttcacagcca acaccggtaa tgtcatcctt cgctgcgctc
     1621 aggatctgat tcgccgagga ccgagatcct tcgctgcgct caggatgaca acttcctacc
     1681 gatcttgctc gtgaaaatac atcatcgcgt ccctatgatg ggttacgctg ctctcttcgc
     1741 cacaatgctc ccacgccctt ttctcgccta tcgccagtca gaccatctgg atatgtgtca
     1801 tcattatgta tagtgtaata ttttgagtca tcataaacta tatattgaca tttcttcgcg
     1861 tccatgctat cctttcgaag gaagaagcat cataccatga actgattgcc acgaatattc
     1921 tgcaagttaa cgtctggcct aatatgattg aagatgagga gagaacccat gatcgatggc
     1981 tcacttatcc ccacctatga cgaactcgcc gcattcatcg ctgacaaatt tgccacgctc
     2041 cgcaagccct acatggaatg ggcaaacctt gcccggcgcg ctgtacaggg gcaaccttat
     2101 gacatgaagc aactcgccac gctggaacgt ttcatcaacg agcaacgagc cgagctacgc
     2161 accatcctta tgattgcctc cgagcatctc gatgaagcgc aactggcact gctccgcgcc
     2221 caggcatgta tgagtaaaaa tgcctggaag tctctcaaaa agcgtgagtt agtgaacctg
     2281 aaggatggtt tcaggctgat tagctattag cattctcgct ggtgtctggc ataagcccga
     2341 tccttcgtag aagccgattg ctccagaatt gacatttcac caggctcagt ctatagtatg
     2401 tgcagtagat acgtccgaaa gacatgtcta ctgcacctta ctgttggaca tatcacctga
     2461 cagtagatgg taactaccat catcgcatcc ccacaaccgc ataaccttga attgtttgtt
     2521 atgtttcagg agcagacgta gtatgtacga attgatgatt ctctcgctat tgatgcgcga
     2581 accagcgcat ggctacagca tcacgaa
//
LOCUS       DASZAK010000355         1624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_185371, whole genome shotgun sequence.
ACCESSION   DASZAK010000355 DASZAK010000000
VERSION     DASZAK010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185371"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VGT44_06235"
     CDS             <1..301
                     /locus_tag="VGT44_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEV2580433.1"
                     /translation="GRILHRLQLNQYFDSLTVSALARHAKPSPTLFELALRRADSIAD
                     YTLHIGDVYVLDILGARSVGITPILLDRQRHLEPDNVDCLLIHSLTEVLDLLEIA"
     gene            495..>1624
                     /locus_tag="VGT44_06240"
     CDS             495..>1624
                     /locus_tag="VGT44_06240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2580434.1"
                     /translation="MADRVGQQLGNYRLVRLLGRGGFAEVYLGEHLRLGTQAAVKVLH
                     TRLASHDDVEGFEKEARTVAHLKHPHIVRVLDYDVEEDTPFLVMDYAVGGTLRHRHPK
                     GTRLPLPTIITYTKQVSDALQYAHDQRVIHRDIKPENMLVEEYDDLLLSDFGIAIVAQ
                     SSRYQNTQNMGGTMAYIAPEQIQGHPRPSSDQYSLGIVVYEWLSGDCPFHGSMTEIVA
                     QHLAASPPSLQEKVPTISSEVEQVVLTALEKDPRKRFHSVKAFATALEQAYQNTQASP
                     ELPQVPLPSSESVTAPGEQALQTERSFTEIAPSPEQPAFQTPHISPLSVSSPSETPSV
                     SPAILSSLSHLPTIPAQSFLPPDESSTEWRTRREAPENVGKET"
BASE COUNT          354 a          515 c          412 g          343 t
ORIGIN      
        1 gggccgcatc ctgcatcgac tgcaactgaa ccagtacttc gacagcctga ccgtctccgc
       61 gcttgcccgc cacgccaagc catcgcccac gctcttcgag ctggccttgc ggcgcgccga
      121 cagcatcgcc gattacacgc tgcacatcgg cgatgtctac gttctcgaca tcctgggcgc
      181 acgctccgtc ggcatcaccc ccatcctgct cgaccgccag cgccacctcg aaccagacaa
      241 cgtcgattgc ctgctgatcc attcactgac cgaggtgctg gatttactgg aaatagcctg
      301 atcgcccgct tccccgttga tgccatgatt catcttttga cgagacacaa agcatagctg
      361 gtaaattagg gtgcgtcctg cgaacgtgat atcattgtgt tgtggatgag gtgtgtttcc
      421 cttttctaaa actcctcatg acaggctatg gcaaagagcg caatgatgag agagactgaa
      481 agcaggtgta aaaaatggct gatcgtgtgg gtcaacaatt gggcaactat cgtctcgttc
      541 gcctcctggg gcgcggcggc ttcgcggaag tgtacctggg cgagcatctg cgcctgggga
      601 cgcaggcggc tgtcaaagta ctccacacgc gactggcgag tcacgacgac gtggagggat
      661 tcgagaagga agcccgtaca gttgcccatc tcaaacaccc ccatatcgtg cgcgtcctcg
      721 actacgacgt cgaggaggat acccctttcc tggttatgga ctacgctgtg ggcggcacct
      781 tgcgtcatcg gcatccgaaa ggaacacgcc tccctctccc aacgattatc acctacacca
      841 aacaagtttc cgacgctttg cagtatgcac acgaccagcg cgtcatccac cgcgatatca
      901 agccagaaaa catgctcgtt gaagagtacg atgatctgct cctctcggac tttggcatcg
      961 ctattgtcgc tcagagttcg cgctatcaga acacgcagaa tatgggtggg acgatggctt
     1021 acattgcccc ggagcagatc cagggccatc cacgcccgtc gagcgatcaa tactccctgg
     1081 gcattgtcgt ctacgaatgg ctcagtgggg actgcccttt tcatgggtcg atgaccgaga
     1141 tcgtggcgca gcacctggcg gcttcaccac cttcgttgca ggagaaggtc ccaaccattt
     1201 cttctgaggt ggaacaggtg gtgctgacgg cactggagaa ggacccgcgc aaacgctttc
     1261 acagcgtcaa agcgtttgcc acagccctcg aacaggctta ccagaacacg caggcatctc
     1321 ctgagcttcc ccaagttccc ttgccgtcct ctgaatcggt aacagcgccg ggagaacagg
     1381 cactccaaac agaacgctcc ttcactgaaa tagccccttc acccgaacag cctgcttttc
     1441 agacgcctca catatcacct ttatccgtgt cttcgccatc agaaacgcct tctgtctctc
     1501 ccgccatcct ttcctccttg tcgcaccttc ccaccatccc tgctcagagc tttctcccgc
     1561 ccgatgagtc ctcaacagag tggcgcacca gacgggaagc tccagagaac gtggggaagg
     1621 aaac
//
LOCUS       DASZAK010000356        11675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_435611, whole genome shotgun sequence.
ACCESSION   DASZAK010000356 DASZAK010000000
VERSION     DASZAK010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435611"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VGT44_06245"
     CDS             <1..1638
                     /locus_tag="VGT44_06245"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEV2580435.1"
                     /translation="DGQQRLTSLYQVTLQGKVVETITPKNKKVKRWFYFDIRKSLDPS
                     IDREEAIVGVPEDKIVRTNFGRDVILDLSSPKLEYATLMFPVSQVFDWDRWQDGFNDY
                     WKGDHNESIRLEFRTFKKQVLENFQLYLVPVITLDSSTSKEAVCIVFEKVNTGGKALD
                     AFELVTAMYAADGYELRKDWYGDDGTKGRHRRFAETLRYPGTEKGIIAEVSNTDFLHA
                     VSLFHTRERRRAAEAEGKQGKDLPAVSGNRQSLLNLPLEAYKRFEAKVERGFIQAAKF
                     LHLLHIYRIFDLPYQSQIIPLAAILADIGDAWEHEANRAMLERWYWNGVFGELYGSAV
                     ESRIARDFMEVPAWLKGGPEPSTVYEATYRADRLKTMRIRISAAYKGVNALLMKVGAQ
                     DFRSGQKFDDTVFFGENVDIHHIFPQDWCKKHGIKPSVFDSIINKTPLSYRTNRIIGG
                     VAPSEYLAKLEKGDSTTPPIDHTRLDRYLISHLIDPSLLHTNNFDAFMMARQKRLLGL
                     IEQATGKPIYSSDIKEEGEDAEVDKDTVEAELTISGV"
     gene            complement(2190..2657)
                     /locus_tag="VGT44_06250"
     CDS             complement(2190..2657)
                     /locus_tag="VGT44_06250"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2580436.1"
                     /translation="MASSLSNSEFTQLASEEQIARTVQALESHGIRAVVFETGEEARN
                     YVLDLIPSGAEVYNSPSRTLELIGLTEEIELTVGGDPTRDKPTVTFAPPTDPDMVGCE
                     LNIRVPDCFAAYEVLRSRGAQFLTAPVDWGYEIRAFLRDPDGHLIELTQSGHQ"
     gene            complement(2784..2915)
                     /locus_tag="VGT44_06255"
     CDS             complement(2784..2915)
                     /locus_tag="VGT44_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580437.1"
                     /translation="MLDLSAFGLDSLLENPRLAEDLDAFARRLLTPLLGYDAAKSQT"
     gene            complement(2959..3669)
                     /locus_tag="VGT44_06260"
     CDS             complement(2959..3669)
                     /locus_tag="VGT44_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEV2580438.1"
                     /translation="MKGQRFRDRTEAGQLLATRLAAYANRPNVLILALPRGGVPVAFE
                     VARALHAPLDVIIVRKLGVPGEEELALGAIASGGVRVLNDDVVRALALSDEVIDTAAA
                     REQQEVERRERLYRGDRPAYEVRGRTVILVDDGIATGATMRAAVVAVRQQQPARIIIA
                     VPVAASATCKEFATEVDDVVCMLRPEDFFAVGYWYEEFSQTTDEEVADLLERASHDRM
                     VAQPHRQRVSSRAMRNAI"
     gene            4015..4770
                     /locus_tag="VGT44_06265"
     CDS             4015..4770
                     /locus_tag="VGT44_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580439.1"
                     /translation="MTAQVETRRYVVGEYPHIAIKNDFGTIRAFRGAHQNEVVVQITR
                     RNRIFGHAPESVHASYKQEKGGNELVISIQRDEFSLATTTTEVDVDIALPGRADLDLV
                     TKAGTITVTDAHGQLASASSAGTITIRHSLLTGDSHISTEAGTIHFEGALEPRGKYQF
                     NTVVGAIHVVLFNTPAFHVDATTTLGSITTNLPGVVVARPSVLGSEAHGDVGMAPHAL
                     LALKSTIGAIELQAWALAATEGSEVSAGQFALG"
     gene            4834..7284
                     /locus_tag="VGT44_06270"
     CDS             4834..7284
                     /locus_tag="VGT44_06270"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV2580440.1"
                     /translation="MIHNASECALRSTIEGQGLSESEATDRRRRGLSNVLPVKTSRTY
                     AQIVRENVLVPVNVIMFALGLALVVLGQVSDALISVGVAFFNVLVGTAQEVRAKRVLD
                     RITLLTRPTATVLRDGQERIVDPRDVVVGDLLVLRPGDQIIVDGRVVSGARLEVDESL
                     LSGEAELVVKQQHDWLYSGSFCASGRACYLAEKVGAESVAGQLALGARAFRRVYTPLQ
                     RQITLILQVMLLVAFFFELLLALAVHDNRVSLVDGVKMAVVVIGIVPKGLLLATSVAY
                     ALGAVRIVGKGALVQQANAIEELSNVDVLCLDKTGTLTANALALERLSPLRLEEARLR
                     QLLGSYVASASVGNLTSAAIGAACPGHPLRVREEVAFSSARKWSAISFDDAQLQGTYV
                     LGAPDILQPSLRERLDRDAEALLQAEIDQGRRVLLFASCSQVEPLHDSHGDPILPHAL
                     DPLGLLSLRDQLRPEAQATLSGFAEAGIQLKVLSGDHPKTVAALAKQAGFAASGSAVS
                     GEDLANMDAVDLAQAADEHAIFGRVTPQQKTALVQALRDRGHIVAMIGDGVNDVLALK
                     QANLGIAMQSGSAATRGVADIVLLEDSFAALPQVFREGQRIRQGMHNILKLFLTRVLY
                     MMGLLVAIMIVDGFPFVPRQNAILTLLTEGIPALALASWARPRAFESKRTFPSLLHFV
                     LPAAGTLGLAALGVFMAAFTSDARLPLAQSALTSFTITSGLLLIPFVVPPARAWVGGS
                     SLEGDWRPTLLSLGLLAAYAVLLAIPPLRAFFALTALDPRSYLLIGLTALAWAVALRW
                     VWRARLLERFLQLDWREE"
     gene            complement(7308..7985)
                     /locus_tag="VGT44_06275"
     CDS             complement(7308..7985)
                     /locus_tag="VGT44_06275"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV2580441.1"
                     /translation="MITQERPDQKQCDEAQSVSAEDRPGPALPPGSIYSDEEALVLGP
                     WQKMRQRLLGPIAYALSRLGISADMLSYSSVLFGLGFCLLAPFQFEVAFWLLAASVVC
                     DGLDGVEARVTHTNTTSGSFTDIFCDVAVVALVAAGLAWKGLIYPALAVLFVYSYTAL
                     ALFLVLHRMLRVSSVGILRPSRMVFFVAIALSFFFHIDLLNVLLLLYLPALPLLFMSF
                     WRLRKAV"
     gene            8181..9140
                     /locus_tag="VGT44_06280"
     CDS             8181..9140
                     /locus_tag="VGT44_06280"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV2580442.1"
                     /translation="MHVITCGEMLIDLVSTEAGVSLAQAPAFKKAPGGAPANVAVGVA
                     RLGYRAGFIGQVGDDEFGHFLADTLQGYGVDTAGLRFTPLARTALAFVSLRADGEREF
                     MFYRHPSADMLWRPEDVDLAYVANCHIFHYGSISLIHEPSRSATLTALDHARSNGALL
                     SYDPNLRLSLWPSPDAARAGILDGWQHAEIIKVSEEELTFLSDKHILESAARSLWHDR
                     LRLLVVTQGSAGCTYFTAEAHGHLPGFAVRPVDTTGAGDGFVAGMLAGLLEGDLTWNT
                     EAIEKALRLGNAVGALSTTQLGAIPSLPTMKEAKAMLERADKQ"
     gene            complement(9141..10643)
                     /locus_tag="VGT44_06285"
     CDS             complement(9141..10643)
                     /locus_tag="VGT44_06285"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HEV2580443.1"
                     /translation="MNTSDVLLPDWLGRCAVNQPGHLAVQAGGVRWSFAELNRQVTSI
                     ARQLAGAEVREGSRVALLAANGLSFVAFVHAMTRLGAVLVPLNTRLTREELTWQVGDA
                     RAIMLVSDERHAGIAAEIERDIVGLRCVMLRAAGDEALLCDLPEADVALRSLIDLDMI
                     QAIMYTSGTTGQPKGAIITYGMQWWNAIGSALNLGHHPGDRWLACLPLFHIGGLAILM
                     RSVVYGISVIVQEKFDVLAVNRAIDEDGVTIISVVAVMLQRMLDARGPGRFYPAALRC
                     VLLGGGPAPRALLEDCAGRGVPVVQTYGMTEACSQAVTLAPADALRKPGSAGRPLLTV
                     QLRIMRDGRQAQEGEPGEIELRGATITPGDADRPDATTQAFHDGWFATGDIGYLDEEG
                     YLYVLDRRADLIISGGENVYPAEVEAVLASHPDVIEAGVCGQPDPQWGHVPVAFVHLR
                     AGSSISAEELMAYAAIRLARYKLPRTIYFTGQLPRNASGKLLRRELLRCK"
     gene            complement(10847..>11675)
                     /locus_tag="VGT44_06290"
     CDS             complement(10847..>11675)
                     /locus_tag="VGT44_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEV2580444.1"
                     /translation="APAPAICGVETALLDALGKTTGQAVSAILAGDGAEPRSGVMVNA
                     VVGAKAIDGAVEEANAAIASGFRCVKLKVGWEQDVAREIERIAAVREAIGTGVHLRLD
                     ANEGWNRAQAMTVLAACARFDIQYVEQPVKAGDLEGMRWLRRAALVPIAADESLAHLE
                     SAYRILDSADILIIKPQLIGGLRASAQLIIDAAALGIGSVVTSSIEAGIGLVAALHLA
                     AAMPQISLECGLATLHLLEDDLLVDELPIRDGFLAVPGGAGLGVRLDRDALERYQAQ"
BASE COUNT         2527 a         3512 c         3280 g         2356 t
ORIGIN      
        1 gatggccagc aacgattgac ctcactttat caagtcacct tacaaggaaa ggttgtcgag
       61 acgatcactc ccaaaaataa gaaggtaaag cggtggtttt actttgacat acgaaaatct
      121 cttgacccat caatagatcg cgaagaggcc attgttggag tgcccgaaga taagattgtc
      181 agaactaact tcgggcgtga cgttattctt gatctttcat cccccaaact ggaatacgcc
      241 acccttatgt ttccggtatc tcaggtattt gattgggaca ggtggcaaga tggcttcaac
      301 gattactgga aaggcgatca caatgaaagc attcgtctag aatttcgtac tttcaaaaaa
      361 caggttctcg aaaatttcca actctacctt gtgccagtca ttaccctgga tagttcaact
      421 tccaaggaag ccgtatgcat tgtgttcgag aaggtcaata ccggcggcaa ggcgctagat
      481 gcttttgagc ttgtgaccgc tatgtatgcc gctgacggtt acgaactgcg aaaagattgg
      541 tacggcgacg atggaacgaa aggacggcat cgtcgttttg cagagaccct tcggtatcca
      601 gggacagaaa aggggattat cgctgaagtt tctaatacgg atttccttca tgccgtttcg
      661 ctgtttcata ctcgtgagcg acggcgcgcg gcagaggcgg aagggaaaca aggcaaggac
      721 ttacctgccg tttcaggcaa tcggcaatcg ctattgaacc tgcctcttga agcatacaag
      781 cggtttgaag caaaggtgga acgcggattt atacaggcgg cgaagttcct gcacctgctt
      841 cacatctatc gcatttttga tctcccgtat caatcccaaa ttatccctct ggcggccatt
      901 ctcgccgata tcggcgatgc gtgggaacac gaagcaaacc gcgcaatgtt ggaacggtgg
      961 tattggaatg gtgtctttgg agagctttat ggttcggcgg tcgaatcgcg gatagctcgt
     1021 gatttcatgg aagtcccagc ctggctaaag ggtggccctg aaccatcaac ggtgtacgag
     1081 gcaacatatc gagccgaccg attgaagacc atgcgcatac gtatttcagc agcctacaaa
     1141 ggcgttaacg ctctcttgat gaaagtaggt gcgcaggatt tccgttcagg tcagaaattc
     1201 gatgacacag tcttctttgg tgaaaatgta gatatccatc acattttccc gcaggactgg
     1261 tgtaaaaagc atggtatcaa gccaagtgtt ttcgattcga tcattaacaa gaccccgctt
     1321 tcctacagga cgaaccgaat catcggcggg gttgctcctt ctgagtatct cgcaaaattg
     1381 gaaaagggcg attcgacaac accgcccatt gatcatacga ggctggatcg atacctgatt
     1441 tctcatctga tagatccatc gcttctgcat actaataatt ttgacgcatt tatgatggcg
     1501 cgacaaaaac gacttctggg gctcatcgag caagcgaccg ggaaacctat ctatagcagc
     1561 gacattaaag aggaaggcga ggatgctgag gttgataagg atactgttga agcagaattg
     1621 acgatctcag gcgtatgagc ctcggagtta aaatatcctg agtaggatat cacagagggt
     1681 gttcaattct cctggcacaa aatgttgtac tcttacaacg gctagccatt atcatgcgac
     1741 aacctctaaa tcgttcactt gtccgcacct aatgcgtctg gttgtcagac gcactgcagg
     1801 aagtagatgt ccgaacgcat gcagataata agggataagc atcatctgga gtaattccta
     1861 cagataaagt gcctgacgag ggagtcggat gggaaggaaa tcctcacttc cattatcttc
     1921 tacccctttc cctttggaac ggtacgggaa gggtgagccc aaaaagcccg gccaggtctt
     1981 gcgtaatctc ccgcaatcgc gttagattaa cggtggttcc ataaatgacg tttcgatggg
     2041 gcgataagga tgcagccgcg ctgacacaaa gcttttctgc ctcatcctct aatgccgcgt
     2101 ccagctcgtg taactgataa tgatgagagg gagccaccag tgcgctgaca gcttccctct
     2161 catcctctta catgctcacc agtcccgctc tactgatgac cgctctgcgt tagctctatg
     2221 agatgaccgt cgggatcgcg caagaaggct cgtatctcat agccccagtc gaccggcgca
     2281 gtgaggaact gggctccgcg cgagcgcaat acctcataag cggcgaagca atcgggtacc
     2341 cggatgttca actcgcagcc aaccatgtcc gggtcggtgg gtggcgcaaa ggtcacagtg
     2401 ggcttatctc tggtaggatc acccccaacc gtcagttcga tttcctcggt caggccgatc
     2461 agctcaagcg tgcgcgaggg cgagttatac acctctgctc ccgatgggat caggtcaagc
     2521 acgtaatttc tcgcttcctc gccggtttca aagacgacgg cccggatgcc gtgagattcc
     2581 agtgcctgca ccgtcctggc gatctgttcc tctgatgcca gttgcgtgaa ttcggaattc
     2641 gatagagaac tagccattga aattactttc actttctttg agggatgccg cgcgcaaaca
     2701 tcccgtttac gcgcggtttg atagtagaat tgctgctgca atcctcacca gattgatgct
     2761 cgcaaagaaa gacggtcgag gggtcatgtt tgtgacttgg cggcgtcata ccccaggagt
     2821 ggagtcagca gcctgcgggc gaaggcgtcc aggtcttcag cgagcctggg attctccagg
     2881 aggctgtcca gaccaaaggc ggagagatcc agcacatact ctgcatattg tgtatcagtt
     2941 cctcagggaa gagtgagttc atattgcatt cctcatcgca cgactggata ccctttgtcg
     3001 gtgcggctga gcgaccatcc tatcatgcga agctcgttcg agcaggtcgg ctacctcctc
     3061 atcggtcgtc tgggagaact cctcgtacca gtacccgaca gcgaaaaagt cctctggccg
     3121 gagcatacat accacatcgt ccacctccgt cgcaaactcc ttgcacgtag ccgaggccgc
     3181 caccgggaca gcgatgatga tgcgggcagg ctgctgctgc ctcaccgcga cgacggcggc
     3241 gcgcatcgtg gctccggtgg cgatcccatc atcgacgaga atgacggtgc ggccacgaac
     3301 ctcgtaggcc gggcgatctc cccggtagag gcgttcgcgc cgctccactt cttgttgctc
     3361 gcgcgctgcg gccgtgtcga tcacctcgtc ggaaagagca agcgcccgca caacgtcgtc
     3421 gttgagtacg cgcaccccac ccgaggcgat ggcccccagc gccagttctt cctccccagg
     3481 aacacccagc ttgcggacga tgatgacatc cagcggcgca tggagcgccc tggcgacttc
     3541 aaacgcgaca ggcacgcctc cccgtggcag cgccaggatc agaacattgg gacgattggc
     3601 atacgccgca agcctggttg ccagcagttg tcccgcttct gtgcgatcgc gaaatcgctg
     3661 tcctttcatg gttgcccatc ccccggttac aagatcagtc agcagactgc cgcagtgacc
     3721 acctcatgct tcttttactc caactgtagt atagggtggc aacctcagag aacggtcaca
     3781 gtgcgacgac tttctctcac aataacgaca caatctctgc ctttatcgga cactgccgcg
     3841 cacttcccct tcaatggtga tcgagccaaa ggctgttatt gtgcgccctt tgtgagcgaa
     3901 taccatcgca ttgtgactgt tctttgaggt tgccacgcta tactaaacct tatactgtgg
     3961 agaaccagca tcgaatggtc tcaagcgagc tttgtgagcg gaaaggacag agtcatgaca
     4021 gcacaggttg agacacgccg ctacgtggtg ggtgagtacc cccacatagc gattaagaac
     4081 gatttcggaa ccattcgcgc cttccgaggc gcacatcaga atgaggtggt cgtgcagatc
     4141 accaggcgca acagaatctt cgggcatgca cccgagagcg tccacgcctc ctacaagcag
     4201 gaaaaggggg ggaatgagct ggttattagc attcagcggg atgagttctc gctggccaca
     4261 actactaccg aggtcgatgt ggatatcgcc cttcctggac gggcggacct ggacctggtg
     4321 acgaaggcgg gaaccatcac ggtgacggat gcacacggcc agcttgcctc ggccagcagt
     4381 gccgggacga ttaccattcg gcatagcctg cttaccgggg actcacacat ctcgaccgag
     4441 gccgggacca tccacttcga gggagcgctc gaaccgcgcg gaaagtacca gttcaacacg
     4501 gttgtgggtg cgatccacgt ggttttgttc aacacacctg ctttccatgt cgatgccaca
     4561 acaacactcg gctcgatcac gaccaatctt cccggcgtgg tcgtggcgcg cccctccgtt
     4621 ctggggagtg aagcgcacgg ggatgtgggg atggcgcctc atgccctgct tgcgctcaag
     4681 agtaccatcg gcgcgattga actccaggcg tgggcgctcg ctgcaactga gggcagcgag
     4741 gtttcggcag gccagttcgc tctgggataa gggctctctg tgctagagga ggtattctga
     4801 ggtgaatcct caaagcggcg acacggagct gagatgatac acaatgcgtc agagtgcgcg
     4861 ctgcgctcaa caatcgaggg gcagggcctt tcggaaagcg aggctacgga tcggcgcagg
     4921 cgcgggctga gcaatgtgtt gcccgtgaag accagccgca cctacgcgca gatcgtccgc
     4981 gagaatgtac tcgtacccgt caacgtcatc atgttcgcgc tggggttggc cctcgtggtt
     5041 ctgggccagg tgtcggacgc gctgatctcg gtcggcgtgg cgttcttcaa cgtgctggtc
     5101 ggcacggcgc aggaagtccg tgcgaagcgc gtcctcgacc gcatcacgct actgacccgt
     5161 cccacagcga ccgtcctgcg cgatggccag gaacgcatcg tcgatccgag agacgtagtg
     5221 gtcggggacc tcctggtcct tcgccccggt gaccagatca tcgttgatgg gcgggtggtg
     5281 agtggggcgc gcctggaagt ggacgaatcg ctcctgtccg gtgaggcgga actggtggtg
     5341 aaacagcagc acgattggct gtattcaggc agcttctgcg ccagcggccg tgcctgctac
     5401 ctggcagaaa aggtcggagc cgagagcgtc gccggccagc tcgccctggg agccagagcg
     5461 ttccgccgcg tctatacgcc cttacagcgg cagatcacct tgatcctcca ggtcatgttg
     5521 ctggtcgcct tcttcttcga gctgctgctg gcgctggccg tgcatgacaa ccgcgtgtcg
     5581 ctcgtggatg gggtgaagat ggcggtggtg gtcatcggca tcgtgccaaa gggtttgctg
     5641 ctggcaacga gcgtagccta cgccctgggg gccgtgcgca tcgtcgggaa aggcgccctg
     5701 gtgcagcagg ccaacgccat cgaggagctg agcaatgtgg atgtgctttg tctcgataag
     5761 accggcacac tcaccgccaa tgcgttggca ctcgagcgcc tctctccttt gcgcctcgaa
     5821 gaagctcggc tgcggcagct gctaggcagt tatgtcgcga gtgcatcggt cggcaatctc
     5881 accagcgcag cgataggcgc agcctgccca ggccatcctc tgcgcgttcg tgaggaggtg
     5941 gcgttctcct cggcgcgcaa atggagcgcg atctccttcg acgatgctca gctccaggga
     6001 acctacgtgc tgggcgcgcc tgacatcctc caaccctccc tgcgcgagcg cctggacaga
     6061 gacgcggaag ctctcctgca agcggaaatc gatcagggta ggcgtgtgtt gctgtttgcc
     6121 tcctgctcgc aagttgagcc tctccacgat agccacggcg acccgatcct gccacatgct
     6181 ctcgatccgc ttggcctcct cagcctgcgc gaccaactcc gtccggaggc ccaggcaacc
     6241 ttgagcggtt tcgcggaagc tggcattcaa ctcaaggtac tctccggcga tcatccgaag
     6301 accgtggcag ccctggcgaa acaggcgggc ttcgcggcga gcgggagcgc cgtctccggg
     6361 gaggatctgg ccaacatgga cgccgtagat ctggcgcaag cagcggatga gcacgccatc
     6421 tttggccgcg tcaccccgca gcagaaaaca gcactcgtgc aggccctgcg cgaccggggt
     6481 catatcgtgg cgatgatcgg cgacggcgtc aacgacgtgc tcgcgctcaa gcaggccaac
     6541 ctgggcatcg ccatgcagag tggcagcgcc gcgacgcgcg gggtcgccga tatcgtcctg
     6601 ctggaggact ccttcgcagc cctgccacag gtgtttcgcg aggggcagcg catccgccag
     6661 ggtatgcaca acatcttgaa gctcttcctg acccgcgtcc tctacatgat gggcttgctg
     6721 gtggcgatca tgattgtgga tggcttcccg ttcgtcccca ggcagaacgc catcttgacc
     6781 ctgctgacgg agggtattcc ggccctggct ctggcctcgt gggcgaggcc gcgcgcattc
     6841 gagtcgaaac gcacatttcc ctcactgctg cacttcgtcc taccagcggc gggaacgctc
     6901 ggcctggcgg ccctgggcgt cttcatggcg gccttcacca gcgacgcgcg actcccgctc
     6961 gctcagagcg cgctgacctc cttcaccatc acgagcgggc tactgctgat tcccttcgtc
     7021 gtcccaccgg cgagggcctg ggtcggggga agcagcctgg aaggcgattg gcgtccgacg
     7081 ctgctctcgc ttgggctgct agccgcctac gcggtgctgc tcgccatacc acccctgcgc
     7141 gcgttcttcg cactgaccgc gctcgaccct cgcagttacc tgctgatcgg cctgacagcc
     7201 cttgcctggg ctgtggcgct gcgctgggtc tggcgcgctc gcctcctcga gcgcttcctg
     7261 caactggatt ggagggaaga atgatcatca tgtgcgttga gactgttcta cactgctttt
     7321 ctcaatctcc agaaactcat gaatagcagc ggaagggccg gcagatacag gaggagcagg
     7381 acattgagaa gatcgatgtg aaagaagaaa gagagcgcga tggcaacaaa gaacaccatg
     7441 cggctagggc gcaagatgcc cacagaagag acgcgcagca tgcggtgcag caccagaaac
     7501 agggccagag cggtatagct atagacaaag aggactgcca gggccgggta gatcagacct
     7561 ttccaggcca gtcccgccgc gacgagtgcg acgaccgcga catcacaaaa gatatcggtg
     7621 aacgagccac ttgtcgtatt cgtgtgcgtc accctcgctt caacgccatc cagtccatcg
     7681 cagaccacag atgccgccag cagccagaag gcgacctcga attgaaacgg agccagcagg
     7741 cagaagccca ggccgaagag cacggagcta tacgaaagca tatctgcgct gatgccaaga
     7801 cgcgagagcg cataggcgat gggaccaagc aagcgctggc gcatcttctg ccaggggccg
     7861 agcacaagtg cctcctcgtc gctataaatg ctgccgggag gaagcgccgg gccaggccga
     7921 tcctcagcag atacggattg agcctcatcg cattgtttct gatctggacg ctcctgggtg
     7981 atcatagtac tttatccctt attaccgaga caagaaccgc ttctctcgaa agagatacca
     8041 tagccgcatc acaatgttct cactgttgca ccgttccagg tcaagatgtt gtcacaaagt
     8101 ttgcgtccat gaggcaatag caaccagcag catcaaggcc gatacggtat actacacata
     8161 aggaaaagag ggcaattacg atgcatgtca ttacctgtgg tgagatgctc attgacttag
     8221 tctcgacaga agcgggggta agcctggctc aggcccccgc tttcaaaaaa gctcctggtg
     8281 gtgccccggc gaacgtcgca gtcggcgtgg ccaggctggg ttatcgcgct ggcttcattg
     8341 ggcaagtggg cgacgacgag ttcggccact tcctggctga cactctgcaa ggttatgggg
     8401 tggataccgc aggtttacgg ttcacccccc tggcacggac ggctctcgct tttgtttctt
     8461 tgcgcgccga cggtgagcgc gagttcatgt tttaccggca tccaagcgcg gatatgctct
     8521 ggcgtccaga ggatgtcgat ctggcctacg ttgccaactg ccacatcttc cattacggtt
     8581 ccatctcgct cattcatgag ccgagccgaa gtgccacctt gacagctctc gatcatgcca
     8641 gaagcaatgg agcgttactc tcctatgacc cgaacctgcg tctctcgttg tggccttctc
     8701 ctgatgcagc gagagcaggc atcctcgatg gctggcaaca cgcagaaatt atcaaggtca
     8761 gcgaggaaga actgaccttc ctcagcgaca agcatatcct tgaaagcgcc gcacgatcac
     8821 tctggcatga tcgtctgcga cttttggtgg tgacacaagg gagcgccggt tgtacgtatt
     8881 ttacggctga agcccatgga cacctgcctg ggtttgcagt ccgcccggta gacactacag
     8941 gagcgggcga cggctttgtc gcaggcatgc tcgcaggctt actggaaggc gacctcacct
     9001 ggaacacaga ggcgatagaa aaggcgctgc gcctggggaa cgctgtcggc gctctgtcaa
     9061 ccacgcaact gggcgctatc ccatcactgc ctaccatgaa ggaagcgaaa gcaatgttag
     9121 agcgtgcgga caagcaatga ctacttgcac cgcagaagct cgcgccgcag cagcttccca
     9181 gacgcattgc gcggcaactg accggtaaag taaatcgtcc tgggcagctt atatctcgcc
     9241 aatcgtatag cggcataagc catcagctcc tcggcgctga tgctgctccc agcgcgcagg
     9301 tgaacgaacg ccaccggcac gtggccccac tgcgggtctg gctgcccgca gacgcccgcc
     9361 tcgatcacat cgggatgcga cgccagcacc gcctcaacct cggcggggta gacgttctcg
     9421 ccgccggaga taatcaggtc agcgcggcga tctagcacat agagataccc ctcttcgtcg
     9481 agatagccga tatcaccagt ggcgaaccag ccatcatgga aagcctgggt cgtcgcatcg
     9541 ggccgatcgg catctcccgg cgtaatcgtc gcccctcgca gctcaatctc ccccggctcg
     9601 ccctcttgcg cttgccgacc atcgcgcata atgcgcagtt ggacagtcaa caacgggcgg
     9661 cctgccgagc cgggcttgcg cagcgcatcg gcaggggcaa gcgtgaccgc ctgcgaacat
     9721 gcctccgtca tgccatacgt ctgcaccacc ggaacaccgc gacccgcgca gtcctccagc
     9781 agcgcgcgcg gagcaggccc gccgcccagc agcacacagc gcagcgcagc cggatagaat
     9841 ctgcccggcc cccgcgcatc aagcatccgc tgtagcatca cagccacaac cgagatgatc
     9901 gttacgccat cctcgtcaat cgcccggttg accgccaata catcaaactt ttcctgcaca
     9961 atcacgctga tgccatacac gacgctgcgc atcagaatag caaggccgcc aatatgaaaa
    10021 agcggcaggc aggccagcca gcgatcaccg ggatgatgtc caagattgag cgcggaccca
    10081 atcgcgttcc accactgcat cccatacgtg atgatagccc ctttcggctg gccggtcgtg
    10141 ccagaggtgt acatgattgc ctggatcata tcgagatcaa tcaagcttct caaagcaaca
    10201 tcagcttcag ggagatcgca tagcagcgcc tcatcgccag cagcgcgcag catcacacag
    10261 cgcagcccta caatatctcg ctcgatctca gccgcaatgc ccgcgtgtcg ctcatcgctg
    10321 accagcatta tcgcccgcgc atcacctacc tgccaggtca gctcctctcg cgtcaagcgc
    10381 gtattgagcg gcacaagcac cgcgcccagg cgcgtcatag catgaacaaa cgcgacaaat
    10441 gacagcccat tcgccgccag cagcgcgacc cggctccctt cgcgcacctc cgctccggca
    10501 agttggcgcg ctatactcgt cacctgccgg ttaagctcgg caaaactcca ccgcacccct
    10561 ccagcctgaa cagccagatg tccaggctgg ttgacagcgc agcgccccag ccaatcgggt
    10621 aacaacacat cagatgtgtt catagtataa ttctctcgcg ttaaagcggg tactttatat
    10681 cgacgaaagt tagtcaggca tacccgtagg tgccgatgat gatttttcgc aaccaaaatc
    10741 attaatgtca tgctgagcga agcgaagcat ctcacagccc atccaccgag acccttcgct
    10801 gcgctcaggg tgacactctc ttaccgaact cggtcgtgaa aatgcatcat tgcgcctggt
    10861 atctctcaag cgcgtcccga tccagtcgta ctccgaggcc agcaccaccg ggaaccgcca
    10921 ggaagccatc acggataggc aactcatcga cgagcaggtc atcctccagc aggtgaagtg
    10981 tggcaagccc acattccagg ctgatctgcg gcatggctgc cgccaggtgc agcgcggcta
    11041 ccaacccaat gcccgcctcg atggaactgg tcacaacgct gccgatgccg agtgccgccg
    11101 cgtcgatgat gagctgcgcg ctcgcccgca ggccgccaat cagctgcggt ttgatgatga
    11161 gtatatcagc gctatcaagg atgcgatacg cgctttccag gtgagcgagc gactcgtcgg
    11221 cggctatcgg aaccagcgcc gcccgcctca gccagcgcat accctccagg tcgcccgcct
    11281 tcaccggctg ctcaacgtac tgaatgtcga accgcgcaca tgcagcgagg accgtcatgg
    11341 cctgtgcgcg gttccagcct tcattcgcat ccagtcgcag atgcacgccc gtcccaatcg
    11401 cctcgcgtac cgccgcgata cgttcaatct cgcgcgcaac gtcctgctcc cacccgacct
    11461 tgagcttcac gcagcggaaa ccgctcgcaa tggcagcatt tgcctcttca accgccccat
    11521 caatcgcttt cgcgccgaca accgcattca ccattacacc tgatcgaggc tccgccccat
    11581 caccggccag gatagcgctc accgcctgtc ccgttgtctt ccccagcgca tccagcagcg
    11641 ccgtctccac cccacaaatc gcaggagcgg gcgcg
//
LOCUS       DASZAK010000357         3800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_399794, whole genome shotgun sequence.
ACCESSION   DASZAK010000357 DASZAK010000000
VERSION     DASZAK010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399794"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="aroF"
                     /locus_tag="VGT44_06295"
     CDS             <1..889
                     /gene="aroF"
                     /locus_tag="VGT44_06295"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV2580445.1"
                     /translation="LECLPGVEQVLIVTKPYKLASREFHPIDTIVEVPVACVAEGVVR
                     IGDEAVVMMAGPCTVETERQVMTTAAAVRNSGAVILRGGAFKPSTSPYGFRGLGEDGL
                     KLLAKARDEFGMAVVTEVMTPGDVGLVCEYADILQIGTRNMQNYYLLDEVGRVDKPVM
                     LKRGMSATIEEWLLAAEYILSQGNPNVILCERGIRTFEKMTRNTMDISAIPLIKKLSH
                     LPIISDPSQGTGRRDLVPMMTLASVAAGCDGLLIEVHPSPDEALKDGAQSLTIAQFQQ
                     LMPQVEAVARAIGRGLVTE"
     gene            1020..1754
                     /locus_tag="VGT44_06300"
     CDS             1020..1754
                     /locus_tag="VGT44_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580446.1"
                     /translation="MVYRLPHFAGRSPLFSASCACLLAALLLISCSSSSTGISSATST
                     VAPGSLLLTPTAPTGISQATSTVVTSLAPVTTPLAPPPQDCAIKPPPQQMHLGGLGLN
                     TSVQLVGGGAFWFYGSFYQNVLHLSQSNSDQRWPMTKWVVEVGPNYSLPVTLRLRNMQ
                     TNTLAWWTDAQTPPRAATQTLVLNPHTDTEDAGSVPGVPDVPHGSPDPGWSEWGLFPV
                     FSAAGCYSLEVSWSGGSWQSIMAVGS"
     gene            2591..3772
                     /locus_tag="VGT44_06305"
     CDS             2591..3772
                     /locus_tag="VGT44_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS domain-containing protein"
                     /protein_id="HEV2580447.1"
                     /translation="MPKRWRLFALIALIILLLGGSVPFLMSWLSTILPAASATVTMTP
                     TNQHLTKTYTVSAVTGTPDASQNQVQARLLSYTTPGKSLTVKATGQSSRGITTATGTL
                     IFSNATQYVTIYAQTFTDYSGVGVVTDTAVDIGPGQTIVVPAHADKPGSTGNVRADDI
                     NATYDVGPNGIWGTVHVENTAPFTGGQDQTYTFVQQSDIDDATPTMVNQLTSDAQASL
                     QQQVRANEELVSSTMQCDPTIKSNHKALDEATDVMLTVTVTCKGEAYDQQATRSLATD
                     LFGSDATSQLGANYALVGDVVIGIPQVLTTGEYGGPVTLSISAEGMWVYQFSDAQKHT
                     FAQLIAGKPLADAQTLLLKQEGVKKVSISTTGGWGAALPTSASDIKFTLVAVPGLQAT
                     P"
BASE COUNT          846 a         1105 c         1048 g          801 t
ORIGIN      
        1 gctggaatgc cttccgggag ttgaacaagt gttaatcgtc acgaaaccat acaagctcgc
       61 cagccgcgag tttcatccaa tcgacacaat tgtagaggtt ccagtcgcct gcgttgcgga
      121 gggagtggtg cgcatcggcg acgaagcggt ggtgatgatg gccggtccct gtaccgttga
      181 aaccgagagg caggtgatga ccaccgccgc cgccgtgcgc aactccggcg cggtgatcct
      241 gcgcgggggc gcgttcaagc cctcaacgtc gccctatggc tttcgcggcc tgggcgaaga
      301 cggtctgaag ctgctggcga aggcgcgcga cgaattcggt atggctgtgg ttaccgaggt
      361 gatgacgccc ggcgatgtcg ggttggtgtg tgaatatgcc gacatcctgc aaatcggtac
      421 gcgcaacatg cagaactact acctgctcga cgaggtcggg cgcgtcgata agccggtgat
      481 gctgaaacgc ggcatgtccg ccacgattga ggaatggctg ctggcggcgg agtatatcct
      541 gtcgcagggc aatcccaatg tgatcctgtg cgagcgcggg attcgcacct tcgagaaaat
      601 gacgcgcaac accatggata tcagcgccat cccgctgatc aaaaagctct cgcacctgcc
      661 catcatttcc gatcccagcc agggaaccgg gcggcgggac ctcgtcccca tgatgacgct
      721 cgcctcggtc gcggcgggct gcgacggcct attgatcgag gtgcatccca gccccgacga
      781 agccctcaag gatggcgcgc aatcgctgac catcgcgcag ttccagcagt tgatgccgca
      841 ggtggaggct gtcgcgagag cgattggacg tgggttggta acagagtagc gagcaaacag
      901 ggcatgattc gccagatgtg tgcggcgctt ccatgcccaa acgttcagat ctttttcagc
      961 aaaagccggt aaactgggca agaaagcgtt gctatgacca aagatagaag gagaaatcga
     1021 tggtttaccg gcttcctcat ttcgccgggc gctcacccct cttctcggca agttgcgcct
     1081 gcctgctggc cgcactgctg ttgataagct gctcttcttc cagcacaggc atttcaagcg
     1141 ccacttcaac ggtagcgccg gggagtttac tcctcacacc cactgcaccc accggcattt
     1201 ctcaggctac ctcaacagtc gtcaccagcc ttgctccggt gacgacaccc ctggctccgc
     1261 ctccccagga ttgcgccatc aagcccccac cgcagcagat gcacctcggc ggcctgggac
     1321 tcaataccag cgtgcaactg gtcgggggcg gagcattctg gttctatggt agcttctatc
     1381 agaatgttct ccatctgagt cagtcaaact ctgatcagag atggccaatg acgaaatggg
     1441 ttgttgaagt cgggccgaac tattcactgc cggtcacctt gcgcctgcgc aacatgcaaa
     1501 cgaatacact ggcctggtgg accgatgccc agactcctcc cagagcagcc actcagacgc
     1561 tggtattgaa cccgcacaca gataccgagg atgccggctc cgtacccggt gtgccagacg
     1621 ttccgcatgg ctctccagat cccggctggt cggaatgggg actcttcccc gttttctccg
     1681 ccgccggttg ttactccctg gaagtgagtt ggtctggcgg ctcctggcag agcatcatgg
     1741 ctgttgggag ctagtggacc accgccctgg ccgggtacag gacaatcgag gacggctcat
     1801 gcggcaatat ataagaatgg ttatcaatca tccttctcgt tgcttttggg gtcccttcct
     1861 cctcgactcc cgatatcatt tctcgaagtg taagtcgctc cgagctctta gagtgtaaat
     1921 ttgtgatcgt cctgttatac tgaagctctt tttgtgatcg ctcggtgaga ttagtgatct
     1981 aatattactc tgtaagagca tgcgtttaaa tgagattaat tcaaccaatg aagaagccta
     2041 caggaaaaga aaggtacagg ggaccatgag cagccaatcg ccctatcgga ctccagagag
     2101 tccttccagg caaaaccaac cacagaggga cacttcactt ggtagtgacc ggtatccacc
     2161 atccacacct tccgcaggct ttccgcagca gcctgaaggt gccgcgcaac agccatatcc
     2221 ttccctaccg tctcaactgg atgtgagcgc atcaccctat cacttctgcc ctcaatgtgg
     2281 aactccactg acatccgatg caacgagttg cagtgcctgt ggaaaaagta ttcctgggca
     2341 agagagccat tatagcggtg aacctgcggc gttgtctgca ctctctgagc atacggtcta
     2401 tggtgcagat gcgcagctat caactgcgct gctcgcccaa atgccgcatg ggtcagcatc
     2461 ccaacaggaa gatgttcctg cgcccacgca gcagccagat acctcgccag gcgcttccag
     2521 ggcaatcccc gcgtatggtg ttgcgcctcc accccctgac aggccatctc ataagggaag
     2581 gcaacggcag atgcctaaaa gatggaggct ttttgcgctg attgcgctca tcattctcct
     2641 tctcggtgga agtgtcccat ttctcatgtc ctggctgtcc accattcttc ccgcagcgag
     2701 tgccacggtg acgatgacgc cgacaaatca gcacctgacc aagacctaca ccgtcagtgc
     2761 agtcacaggg acacccgatg cctctcagaa tcaggtgcag gcgcgcctcc tctcctatac
     2821 cacaccgggg aaatctctca cagttaaggc aactggtcag agtagtagag gcattactac
     2881 agcaacaggg actttgatct tttccaatgc tactcagtac gtcacgattt acgcccagac
     2941 cttcaccgat tacagtggag taggtgtggt aaccgacacg gctgtcgata ttgggccagg
     3001 tcagacaatc gtcgttccag cccatgccga caagcctgga tcgactggca acgttcgggc
     3061 agacgatatt aacgcaacct acgatgttgg gcccaacggt atctggggta cagtccatgt
     3121 ggaaaacacg gcccccttta ctggcgggca ggatcagacc tacacgtttg tgcaacaaag
     3181 tgacatcgat gatgcgaccc ctaccatggt gaaccagctc acctctgatg ctcaggcgtc
     3241 cttgcagcag caggttcgcg cgaacgaaga actcgtctcc agcactatgc aatgtgaccc
     3301 taccatcaaa tcgaaccata aggcccttga cgaggcgacc gatgtcatgc tgacggtcac
     3361 tgtcacatgt aagggtgaag cctatgatca gcaggcgact cgctctctgg caaccgattt
     3421 gttcggaagt gatgccacct ctcaactggg tgctaactat gccctggtag gtgacgtggt
     3481 gatcgggata ccgcaagtgc tgacgaccgg tgaatatggc ggcccggtca cattgagcat
     3541 cagcgcagag ggcatgtggg tctaccagtt cagtgatgcc cagaagcaca ccttcgccca
     3601 gttgattgcg ggaaaacctc tcgcagatgc ccagactctc ctgctcaaac aggaaggggt
     3661 caagaaggtt agcatcagta ctactggagg gtggggggcc gcgttgccaa cttctgcaag
     3721 cgatatcaag tttactctgg tggctgtgcc aggattacag gcaacaccat gaaggacacc
     3781 tgtccagcac gggggagggg
//
LOCUS       DASZAK010000358         3651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_80373, whole genome shotgun sequence.
ACCESSION   DASZAK010000358 DASZAK010000000
VERSION     DASZAK010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80373"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1821
                     /locus_tag="VGT44_06310"
     CDS             <1..1821
                     /locus_tag="VGT44_06310"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEV2580448.1"
                     /translation="RGFRDSIEIGRENRYELYDLMLEMPQPLVPRYLRFDAPQRTLSD
                     GTTLHELDAAYVEQLTRELVANKIEALAVAFVNSFANGSAEREARAVIQEIAPEMRVA
                     VSSEVVPEIREFERTSTTIINVYVQELVERYLRELQARLARMGFDGSLFLMISSGGIV
                     TVETAIRFPVRLLESGPAAGALAAASYGAACGFADLLSFDMGGTTAKFCVIDQGQPLT
                     ALEFEVDRRYRFKKGSGLPVKVPVIEMIEIGAGGGSIASIDSLGLLKVGPHSAGASPG
                     PACYGRGGSEPTVTDADLVLGYLDPDYFLGGQLKIDLAAARQAIQTRIADRAGLAIEE
                     AAWGIHQIVNESMANAARIHTLERGKDPHGLPVFAFGGAGPVHGFRIAKALGSSALIV
                     PFGAGVMSAVGFLTAPLAFDFVRSWRGRIDHLDWQKANALLREMEAEGQALLEASGVP
                     TGQISYQREADIRYVGQGYEIRVPLPAGELDSGSVSTITASFEEVYRRLYERLSQSVP
                     IEIINWRVTTFSPMPTVRLQLPEHTADQSARKGSRKAYFPELGGYWDTPVYNRYGLSP
                     GTSFGGPAIVEERESTVIVGPDSRFRIDDQWNLIVEFQAK"
     gene            1836..3584
                     /locus_tag="VGT44_06315"
     CDS             1836..3584
                     /locus_tag="VGT44_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEV2580449.1"
                     /translation="MTNTLDPIMLEVLWNRLLSVVNEQQVTLMRTAFSTVVRESQDLA
                     CGVFDTRGSMIAQSLTGTPGHINAMATGVRHFLQAYPKETLWPGDVLLTNDPWQTAGQ
                     INDMTILMPVFKGERLVAYFASTCHSPDIGGRIFSAEAREVYEEGLRIPITKLFARNE
                     PNHELLKIIRANVRTPNETIGDLYAQISSGAVGGRELLHFMEEFGLESIDPLADEIIS
                     RSERAMRDAIRTLPNGRYENESWSDGFDEPIRIKVCVTIEDEDILIDFAGSSPQSPWG
                     INVVLNYTYAYASFAIKAAVSPEVPHNAGAFRPVHVSAPPGCILNCLEPAPVASRHMI
                     GHFLPGVIFGALAPAMPGRLIASGADPIWLSVWHGKWPVTQESFTFNLFQCGGTGARA
                     TKDGLNTTGFPSGVAGVPAEVIESLSPLIQYRRELRTDSGGAGTFRGGLGQWTEHGYR
                     GNLPWSVSALADRTRFPANGLEGGKDGAMGEFIVNEDARPQPKAMVTFEPGDHVHLNL
                     PGGGGYGDPLQRLPERVLSDVVNGYVSLEAAEREYGVVIRYLGSPQQLVRPPELYVID
                     ESATAALRAGRGAIGV"
BASE COUNT          677 a          994 c         1209 g          771 t
ORIGIN      
        1 cgcggcttcc gtgattcgat tgagattgga cgagagaacc gctacgaact ctatgacctc
       61 atgttagaga tgccgcagcc gttggtgccg cgctacctgc ggttcgacgc gcctcaacgc
      121 actctgtctg acgggacaac gctgcatgag cttgacgctg cctatgtcga gcagttgacg
      181 cgcgaactgg tggccaacaa gatcgaggcg ctggcggtgg cctttgtgaa tagcttcgcg
      241 aatggcagcg ccgaacgaga ggcgcgcgcc gtgatacagg agatcgcgcc ggagatgcgc
      301 gtagcggtat cgtcggaggt tgtgcctgaa attcgcgaat ttgagcgcac ttcaacgacc
      361 attatcaatg tgtatgtgca ggagcttgtc gagagatacc tgcgcgaatt gcaggcgcgc
      421 ctggcgcgta tgggattcga tggcagcctc tttttgatga tttccagcgg cggcatcgtc
      481 acggtggaga cggccatacg cttccccgtg cgcctgctcg aatcgggtcc ggcggcggga
      541 gcgctggcgg cggcgagcta tggcgcggcg tgcgggtttg ctgacctgct ctcgttcgat
      601 atggggggga cgaccgccaa gttttgtgtg attgaccagg gccagccact gacagccctc
      661 gaatttgagg ttgaccggcg ttaccgtttc aaaaaggggt cgggactgcc ggtgaaagtg
      721 ccggtgattg aaatgatcga gataggcgct ggtggcggct cgattgcctc tatcgactcg
      781 ctggggttgt tgaaagtagg accgcattcg gcaggcgctt cgccgggacc ggcctgctat
      841 gggcgcgggg gaagcgagcc gacggtgact gatgccgacc tggtgttggg gtatcttgac
      901 cctgactatt ttcttggtgg tcaactcaag atcgatcttg ctgccgcgcg acaggccatc
      961 cagacgcgca ttgccgatcg tgccgggctg gccattgaag aggccgcctg gggcattcac
     1021 cagatcgtga atgagagcat ggcgaacgcg gcgcgcattc acacgctgga acgtggcaaa
     1081 gacccgcatg gcttgccggt cttcgccttc ggtggcgcgg ggccggtgca tggttttcgc
     1141 attgccaaag cgctgggttc atccgccttg atcgtcccat tcggggctgg cgtgatgagc
     1201 gcggtcggct tcctcactgc tccgcttgcc tttgattttg tgcgttcatg gcgcggtcgc
     1261 atcgatcacc tggactggca gaaggctaat gcgctgctgc gtgaaatgga ggcggaaggc
     1321 caggcgctgc tggaggcatc gggagttcca accgggcaaa ttagctacca gcgtgaggcg
     1381 gacatccgtt acgtcggcca gggttacgag atccgcgtgc cacttccggc aggtgaactt
     1441 gacagcggaa gtgtttccac gatcactgcc tcatttgaag aggtctaccg ccgtctttac
     1501 gagcgattga gccagtcggt gccgattgaa ataatcaact ggcgtgtcac cacattcagt
     1561 cccatgccta cggtgcgttt gcaacttcct gagcatacgg cggatcagtc ggctcgcaaa
     1621 gggagccgaa aggcgtattt ccccgaactg ggcggctatt gggacacgcc ggtctacaat
     1681 cgctatggcc tgtcgcctgg cacgagtttc ggcgggccgg ccatcgttga ggagcgcgag
     1741 tcaacggtga tcgtggggcc ggacagtcgt tttcgtattg atgaccagtg gaacctgatt
     1801 gttgagttcc aggcgaagta aaaggacttg ctatcgtgac gaataccctt gacccgataa
     1861 tgctggaagt actgtggaat cgcctgctct cggtggtgaa tgagcagcag gtcacgctga
     1921 tgcgtacggc tttcagcact gttgtgcgtg agtcgcagga cctggcgtgt ggggtctttg
     1981 atacgagggg ctcgatgatt gcccaatcgc tgaccggcac tcccggtcat atcaacgcca
     2041 tggcaaccgg agtgcgccac ttcttgcagg cgtatccgaa ggaaacgttg tggccggggg
     2101 atgtgctgct gacgaatgat ccctggcaaa ccgccggcca gatcaacgat atgactattt
     2161 tgatgccggt cttcaaggga gaacgcctgg tggcgtactt cgccagtacg tgtcactcgc
     2221 ctgatattgg agggcgcatc ttctcggctg aggcgcgcga ggtctacgag gaggggctgc
     2281 gcattcccat caccaaattg tttgctcgca atgaacccaa tcatgagctg ctcaaaatta
     2341 ttcgcgcaaa tgtgcgcaca cccaacgaaa cgattggcga cctctacgcg caaatctctt
     2401 caggcgcggt aggcgggcgc gaactgctac acttcatgga ggagtttggg ctggagagca
     2461 tcgatccact tgccgacgag atcatttcgc gttcggaacg agcgatgcgc gatgcgattc
     2521 gcacgctccc caacgggcgc tatgagaacg agtcctggag cgacggcttt gatgaaccca
     2581 tccgcatcaa ggtgtgtgtg acgatcgagg acgaggatat cttgatcgac ttcgcgggat
     2641 cgtcgccgca gagtccgtgg ggcatcaacg tggtgctgaa ctacacgtat gcctacgctt
     2701 cgttcgcgat caaggcggct gtcagcccgg aggttccgca taacgcgggg gcgtttcgtc
     2761 cggtgcatgt cagcgcgcct cccggctgca tactgaactg cctggaacct gctcccgtcg
     2821 cttcgcgtca tatgatcggg catttcctgc ccggtgtgat ctttggagcg ctggccccgg
     2881 cgatgccagg acggctgatc gccagtgggg ctgacccgat ctggttgtcg gtctggcacg
     2941 ggaaatggcc ggttacccag gaatcgttca cgtttaacct gtttcagtgt ggcggcaccg
     3001 gggcgcgcgc gacgaaggat ggcctgaata ccacgggctt ccccagcggc gtcgctggcg
     3061 tgcctgctga ggtgatcgaa agcctgtctc cgctgatcca gtatcgtcgt gagctgcgga
     3121 ccgattcagg gggcgccgga accttccgtg gcggtctggg acagtggacc gagcatgggt
     3181 atcgtggcaa tctgccctgg agcgtctcgg cgctggccga tcgcacacgg tttccggcga
     3241 acgggctgga gggcggcaaa gacggcgcga tgggcgaatt tatcgtgaat gaggatgcgc
     3301 gcccgcagcc aaaggcgatg gtgacgtttg agccaggcga ccacgtgcat ctcaacctgc
     3361 cgggcggcgg cggctatggc gaccccttgc agcgcctgcc tgaacgagtg ctgagcgatg
     3421 tggtcaatgg ctatgtatcg ctcgaagcgg cggagcggga atacggcgtg gtcattcgct
     3481 acctgggcag cccgcagcag cttgtgcgac cgcctgaact gtacgttatt gacgagtcag
     3541 caacggcggc gctacgagcg gggagaggag caattggcgt gtaggggcgc aattcattgc
     3601 gcccgcgtac ttcctcgcat cggcctggca agcgggcgca atgaattgcg c
//
LOCUS       DASZAK010000359         3168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_541282, whole genome shotgun sequence.
ACCESSION   DASZAK010000359 DASZAK010000000
VERSION     DASZAK010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541282"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VGT44_06320"
     CDS             <1..1032
                     /locus_tag="VGT44_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2580450.1"
                     /translation="APVDFGLAATLSLLLFIAIAVALLVQAGILRSRSFQVISGRTRP
                     ARTIDLRFWRWPAFILCVVIFLLALVVPLGMCVLLSFLHAFGLGITPGNFTLDNYTAV
                     FTSSSNDLAALTRTIWLAFGTASVTALIGLPLAFIIRRTTLPGRRLLSLFTLVTIAVP
                     GIILACGYIFAWNATYLENIGIGGVNGIKFYGTLWILFAAYIGGSLPYATRLGIGALE
                     QVGQNMLDAARVQGARTFQLLARIVAPLLRANLISVWLLVFTGTMFELAASELLYPPG
                     QPTLPVQIVGYFNNFQLGPGMALAMLDVALVTLFLIVLRAIPWLFQRLVKFGARRRMN
                     YVSTDSATH"
     gene            1004..2107
                     /locus_tag="VGT44_06325"
     CDS             1004..2107
                     /locus_tag="VGT44_06325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580451.1"
                     /translation="MFLQIQQLTKSYSNRTVVSGIDLEIEEGEIISLLGPSGCGKTTI
                     LRMIAGLVSPDKGRIQIGDKLVYDGKREMPVEERDLGMVFQDYALWPHMTVARNISFG
                     LRLRRVPAKMIAERVQSLLELVNLSGMGDRYPFQLSGGQQQRVAVARALATQPRLVLL
                     DEPLSSLDTALREEVGSELVQLFKRLKITTINVTHDQNEAMNMSDRIIVLREGKVQQI
                     GTPRELYLLPANAFVASFMGQANMFSGEIIKNLSKEDTISLRLLPENDHSWVIAGKVR
                     AEVSSQLNGHAMLFCRPDDVRIWQEEQLNGQQNIVPGTVQHASFVGGRWRTLIKYGEQ
                     QDRTVLAFATTQPSLNAKVWLELPPEQCQIVPV"
     gene            2224..>3168
                     /locus_tag="VGT44_06330"
     CDS             2224..>3168
                     /locus_tag="VGT44_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2580452.1"
                     /translation="MICPNCGNELDQNEVFCGQCGAQTLPSFKPTEMVQTPQPQAQSS
                     LLQGGYSTQPITGASYAPGTPGAQSSRLPANASGALPPTAPASIPSGPQQQGNFYQDA
                     TESISALPPMQNYPAAYPPSSAQYGQAVQTNNYSGPQYPTAYPTGTGYSARPGPTPPP
                     RQRSSAVLIIAIICLGLAIISFGAFGVLYYLHSKNQPIAQTTPSPAITVTTVPSPTPS
                     PTPSPTPSPTPSLTPSPSPAVSPTPSVPPPDNGFTYCTDPCAARGFVTEVPQGWLQSA
                     PDTTTVIFNSPTQNDVLATFRTPGQSSATADVLVTNDLN"
BASE COUNT          640 a         1023 c          848 g          657 t
ORIGIN      
        1 gcccccgtcg attttggcct ggctgctacg ctgtccctgc tgctctttat cgccattgcc
       61 gtcgcgctgc tcgtgcaggc gggcatcctg cgctcgcgct cgtttcaggt tatcagcggg
      121 cgcacccgcc cggccaggac catcgacttg agattttggc ggtggccagc ttttatactg
      181 tgtgtggtta tcttcctgct ggccctggtc gttcccctcg gcatgtgcgt gctgctctcg
      241 ttcctgcatg ccttcggcct gggcatcaca cccggcaact tcacactgga caactatact
      301 gccgtcttca ccagcagctc caatgacctc gcggcgctca cccgcaccat ctggctcgcc
      361 ttcggcaccg ccagtgtcac cgcgttgatc ggcttgccgc tggcctttat cattcgccgc
      421 acaacgctgc ccggtcgccg gctgctcagc ctgttcaccc tggtcacgat cgccgtgccg
      481 ggcattatcc tggcctgcgg ctatattttc gcctggaatg cgacctatct ggagaacatc
      541 ggcatcgggg gcgtaaacgg catcaaattt tatgggacgc tctggatact cttcgcggca
      601 tacatcgggg gcagcctgcc ctacgccacg cgcctcggta tcggcgcact cgagcaggtt
      661 ggacagaata tgcttgatgc cgcgcgtgtg cagggggcgc gcaccttcca gttgcttgcg
      721 cgaatcgttg cgccgctgct gcgcgccaac ctgatttccg tctggctgct cgtcttcacc
      781 ggtacgatgt tcgagctggc agcctcggaa ctgctctacc cacccggtca acccacgctg
      841 ccggtgcaga tcgttggcta cttcaacaac ttccagctcg gccccggcat ggccctggca
      901 atgctcgacg tggcgctggt cacgctcttc ctgattgtat tacgcgcgat tccctggctg
      961 ttccagcggc tggtcaaatt cggtgcgcga agaaggatga actatgtttc tacagattca
     1021 gcaactcact aaaagctaca gcaaccgcac ggtggtctca ggtatcgatc tggagattga
     1081 ggaaggcgaa attatctctt tactggggcc atcgggctgc ggcaaaacga ctatcctccg
     1141 catgatcgcc ggcctggtca gtcccgacaa gggacgcatc cagatcggcg acaagctggt
     1201 ctacgatggc aaacgagaga tgccggttga agagcgtgat ctgggcatgg tcttccagga
     1261 ttacgctctg tggccgcaca tgaccgttgc gcgcaatatc tcctttggcc tgcgcctgcg
     1321 ccgggtcccg gcgaaaatga ttgctgagcg ggtacaatcc ctgcttgagc tggtcaatct
     1381 ctccggtatg ggcgaccgct atcccttcca gctctcaggc gggcagcagc agcgcgtcgc
     1441 ggtcgccaga gcgctcgcca cacagccgcg cctggtgctg ctggacgagc cgctctccag
     1501 cctggacacc gctttgcgtg aggaagtcgg ctcggaactg gtgcagctct tcaaacgcct
     1561 gaagatcacc accatcaatg tgacccacga ccagaacgag gccatgaata tgtccgaccg
     1621 catcatcgtg ctgcgcgagg gcaaagtgca gcagatcggc accccacgcg aactttacct
     1681 gctaccggcc aatgccttcg tcgcatcttt catgggccag gcgaacatgt tctccggcga
     1741 gatcatcaag aatttgagta aagaggacac gatctccctg cgtctcctac cggaaaacga
     1801 ccactcatgg gtcatcgcag gtaaagtgcg cgcggaggtc agcagccagc tgaacggaca
     1861 cgccatgctg ttctgccgtc cagacgacgt gcgaatctgg caggaagaac agctcaacgg
     1921 ccagcaaaat atcgtccccg gcacagttca acacgcctca ttcgtgggcg gacgctggcg
     1981 aacgctcatc aagtatggcg agcagcagga tcgtaccgtg ctggccttcg cgacgacgca
     2041 gccgtcgctc aatgcaaaag tctggttaga actgcccccc gaacaatgtc agatcgttcc
     2101 cgtctgaaaa gagtagctat cgtaggacaa atgcgctata atagacgccg gggaaaatcc
     2161 cccggcgtta gttattatga tgagtaattc ataaaacagc atgtgtttat agaggtatcg
     2221 ggaatgatat gtccaaactg tgggaatgag ttagatcaga acgaggtctt ctgcggtcaa
     2281 tgtggcgcac agaccttgcc ttcattcaaa ccaaccgaga tggtccagac gccccagccg
     2341 caagcccaga gtagtttact ccagggcggg tacagtaccc agccgattac aggagcaagc
     2401 tatgctccgg gtacacctgg cgcacagagc agcaggttgc ctgccaacgc gagcggagcg
     2461 ctaccaccaa ccgcgcctgc cagcatacca tccggcccac agcagcaggg caacttttac
     2521 caggatgcga ctgagtccat ttcggcgctc cctcccatgc agaactaccc ggcggcctat
     2581 ccaccctcgt ccgcacagta tgggcaggct gtacaaacga acaactactc cggtccacaa
     2641 tatccgaccg catatcccac cggcactggc tatagcgccc gacctggtcc gacacctcct
     2701 cccagacagc gcagtagcgc cgtcttgatt attgcgatca tctgtctcgg ccttgctatc
     2761 atctcctttg gcgcgtttgg cgttctctat tacctgcaca gcaagaatca gcccatcgcg
     2821 cagacgacac cgtcaccggc gattacggtc acgactgtgc caagcccgac tcctagccca
     2881 acgccaagtc caacacccag cccgacgcca tcgttgacgc cctcgccctc gccggcggtc
     2941 tcgcctacac cgagcgtacc accccccgat aacgggttca cgtattgcac cgatccatgc
     3001 gcggcacgag ggtttgtcac tgaggttccg cagggctggt tacaatccgc gcccgatacc
     3061 acaacggtta tcttcaatag ccctacacaa aatgatgtcc tcgcgacctt ccgcacaccg
     3121 gggcaatctt cagctactgc cgatgtcctg gtgacgaacg atctgaac
//
LOCUS       DASZAK010000360         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_470417, whole genome shotgun sequence.
ACCESSION   DASZAK010000360 DASZAK010000000
VERSION     DASZAK010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470417"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VGT44_06335"
     CDS             <1..224
                     /locus_tag="VGT44_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580453.1"
                     /translation="SQPALEHEQESVEAIQKSPVISGSSAPIQTALGRPDQGTSLDVT
                     RPQRDAPNLYGQTALGRPDQEASHDVTRP"
     gene            824..1411
                     /locus_tag="VGT44_06340"
     CDS             824..1411
                     /locus_tag="VGT44_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2580454.1"
                     /translation="MPNRDDAHTLMTTHVKNENLRKHMYSVEAAMRFYARKYGEDEEI
                     WAITGLLHDADYEEYPDLREHTQVLAGWLRDGGYDERITYAILAHNDLNVATHPRDNL
                     LSHALFACDEVTGMITATALVRPNKSIYGLEVSSVRKKMKTKGFAAGVHREDLVNGAA
                     ELGVDLDEHIAFVIQAMSSVAETLGLAGIEESAAL"
     gene            1431..1838
                     /locus_tag="VGT44_06345"
     CDS             1431..1838
                     /locus_tag="VGT44_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580455.1"
                     /translation="MQQQSTTQTKQRLFTATEVAQFEYCPLVWWHEQFGPLAHMDTEE
                     LFARMVELEHEYGQQAVAMPEYEAIENLLLRQGAFEDGRRQHEEHAEEVEKIEEETRI
                     STGRGRGARVLVVLAIVLVILALVMIVAAVLVR"
     gene            1846..>2253
                     /locus_tag="VGT44_06350"
     CDS             1846..>2253
                     /locus_tag="VGT44_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580456.1"
                     /translation="MLNNTLLAIGAGLLLAAAALGLLLLNERRGRRSRLIVERHRALG
                     LPEGELVYEDADGQGESLTSRAYPLVGKPEYVVKLPDGRPVPIELKLNVTDVTAPHSN
                     HVVQLGAYCLILEEYFERPPTYGIVRYADSEFTV"
BASE COUNT          525 a          658 c          640 g          430 t
ORIGIN      
        1 gcagccagcc cgcgctcgaa cacgagcaag aaagcgtcga agccatacag aaatctcctg
       61 tgatttctgg ctcaagcgct ccaattcaaa ccgccctcgg acgacccgat caggggacat
      121 ccctggatgt taccaggcca cagcgcgacg caccgaacct ctatggccaa accgccctcg
      181 gacgacccga tcaggaagca tctcacgatg tcaccaggcc ataaccagga agcgagactc
      241 tctaactgta gttctctaca ctaacacgcc ccccatcatg aatggaattt gatgggggcg
      301 tgttagtgta tctctgggat cattcatgat gcaagctgct cggatctcct actccagtaa
      361 caagcgttcc cgttggacca gatgctaacg ggttcacctg gcacagaaat cgctacgatc
      421 cagcaacggc ctttccgccg attcttccaa ctgatacgat caagaacatt accattatct
      481 ttgatgaagg agtcgatacg ggtccagatt ttaagggatg gaccttcctc gataacatcg
      541 atatcaacgg caccttgatt gctcaggggc cggtcggcta gcagaataga gcgatctttc
      601 cagagggcga ccacaagggt gcgcccttac catgacccgt cggatggcga cgcgagagcc
      661 atttcgcagt catggtaagg gcgcaccctt gtggtcgccc tctggaatct catcaatgcc
      721 aagctttagg gcacactcac ctctaaatga gtacttggaa gccatgttat aataatcgtc
      781 acgaataacg ttacttattg ctggactgga gtatttggaa cgtatgccaa atcgagacga
      841 tgcccacacc ctcatgacca cccacgtgaa aaacgaaaac ctgcgcaagc acatgtactc
      901 ggttgaggcc gccatgcgct tctacgcgcg caagtatggc gaggacgagg aaatatgggc
      961 cataacgggt ttattgcacg atgctgatta cgaagaatat cccgatctgc gcgaacatac
     1021 ccaggtgctg gccggatggc tgcgcgacgg cggctatgac gagcgtatca cctacgccat
     1081 cctcgcgcac aacgacctga acgtcgccac gcacccgcgc gacaacctgc tctcccacgc
     1141 cctcttcgcc tgcgacgaag tcacgggcat gattaccgcg accgcgctgg tgcgtcccaa
     1201 caagagcatc tacgggctgg aagtctcttc cgtgcgtaag aagatgaaga cgaagggctt
     1261 cgcggcgggc gtacatcgag aagacctggt caacggcgcg gcggaactcg gcgtagacct
     1321 ggatgagcat atcgccttcg ttatccaggc catgtccagc gtcgccgaaa cgctcggcct
     1381 cgccggaata gaagaatcgg cggcattata accgggtttc atggaatgtg atgcaacagc
     1441 aatcgacaac gcaaacaaaa cagcgcctct ttaccgcgac cgaggtggca caattcgaat
     1501 actgtccgct ggtatggtgg cacgagcaat tcgggccgct ggcgcacatg gacacagaag
     1561 agctgttcgc ccgcatggtg gaactggagc atgagtatgg ccagcaggcc gtcgccatgc
     1621 ccgaatacga ggcgatcgag aatcttctgc tgcgccaggg cgcctttgag gacgggcgca
     1681 ggcaacatga agaacacgcc gaagaggtag agaagatcga ggaggagacg cggatcagca
     1741 ccggcagagg tcggggggcg cgcgtgctgg tcgtcctggc gattgtgctc gtcatcctgg
     1801 cgctggttat gatcgtcgcg gcagtgcttg tgaggtaagt gagctatgct gaacaacacc
     1861 ctgctggcta tcggagccgg tctgctgctg gccgccgccg cgctggggct gctcctgctc
     1921 aacgaacggc gcgggcggcg ctcgcggttg atcgtggagc ggcatcgcgc gctgggactg
     1981 ccggaaggcg agctggtcta cgaagatgcc gacgggcagg gcgagtcact cacctcgcgc
     2041 gcctatcccc tcgtgggcaa gcccgagtac gtggtgaagc tgcccgatgg ccgcccggtt
     2101 cccatcgaac tcaaactgaa tgtcaccgat gtaaccgcgc cgcatagcaa ccatgtcgtg
     2161 caactcggcg catactgcct gatcctggaa gaatatttcg agcgcccgcc cacatatggc
     2221 attgtacgat atgccgatag cgaatttacc gtc
//
LOCUS       DASZAK010000361         1668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_577203, whole genome shotgun sequence.
ACCESSION   DASZAK010000361 DASZAK010000000
VERSION     DASZAK010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577203"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="VGT44_06355"
     CDS             <1..787
                     /locus_tag="VGT44_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV2580457.1"
                     /translation="LSTLASNPLFQRHYPALALAAASVSTPQLRNMGTLGGNLLLDTR
                     CNYYNQTEFWRQSSGYCMKKDGDVCLVAPGSPRCWAISSADTAPVLVSLDARARLVSA
                     RGERIVPVRELFRDDGMHPYTKAWDELLTEVLLPPAAGWRSTYLKLRRRGSFDFPILG
                     VAAALRFADDGTVSDARLTLGAVASHPVEAHEAAGQLLHQQLSPELIETVAVAAAKRA
                     KPLDNADLTINYRKQVTPVFVRRALEELMQAGVIEGRVGERTR"
     gene            994..1497
                     /locus_tag="VGT44_06360"
     CDS             994..1497
                     /locus_tag="VGT44_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV2580458.1"
                     /translation="MGTSLSKTQLLTELRTEQAAWEALLDEIGQAHMTQPEVAGGWSI
                     KDIVAHLTGWRRRSVRRFQALLKHEPDFSPPWPPELREDDEINAWIYEANRHRPLAEV
                     LSESRQVFQQLVETLDAFSEDEFQNLRRILGLETEQVSGSMFFAHFHQEHEPDMRVWL
                     EKVKKAG"
BASE COUNT          352 a          484 c          511 g          321 t
ORIGIN      
        1 gctctccact ctggcaagca acccactttt ccagcgtcac tatcccgcgc tggccctggc
       61 agcagcgtcc gtttcaacgc cacaactgcg caacatgggc acgctgggtg gtaatctcct
      121 gctcgatacc cgctgcaact actacaatca aaccgagttc tggcggcagt ccagcggcta
      181 ttgtatgaag aaggacggag atgtgtgcct ggtcgcgccg gggagtccgc gctgctgggc
      241 catctcctcg gcggataccg cgcccgttct ggtgagcctg gatgcccgcg cacgcctggt
      301 aagcgcacgc ggagagcgca tcgtacccgt gcgtgaactc tttcgcgacg atggtatgca
      361 cccctataca aaggcctggg atgaactgct caccgaggtc ctcttgccac cggcagcagg
      421 ttggcgtagc acgtatctga agctacgcag gcgcggctct ttcgatttcc ctatcctggg
      481 cgttgccgcc gcgttacgct tcgccgacga cggcacggtc tctgatgcgc ggctcacgct
      541 gggcgcggtc gcctcgcacc cggtcgaggc acatgaagca gccgggcaac tccttcacca
      601 gcagctcagc ccggaactga tcgagactgt ggctgtggca gccgctaaac gtgccaagcc
      661 gctcgataac gcggatctga cgatcaatta ccgtaaacag gtgacgccgg tatttgtacg
      721 gcgggcgttg gaagaattga tgcaagccgg ggtgatcgag gggcgggtgg gggagcggac
      781 acgctaaatc gcatcctggg atttacagtc tagccgaccg ccagagtaat gggtcttccg
      841 cgacgtgaag ccgcttgcga gactcggacc ctggctcttg aggatgaacg gatcggatta
      901 caacgcgagc gctatgaggg cgacttcttc ccgctttttg tgctactctg tgaggaaatg
      961 gacacacacc gtcccgtatg aaaggaaagc agcgtgggaa cttctctcag caaaacgcaa
     1021 cttctcacgg aattgcgcac ggaacaggcg gcctgggaag ccttgctcga cgagattggg
     1081 caagctcata tgacgcagcc agaggtcgct ggtggctggt cgatcaagga tatcgtggct
     1141 cacttaacgg gctggcgccg ccggtcggtt cggcgcttcc aggcgcttct gaagcatgaa
     1201 ccagactttt caccgccctg gccgccagaa ctgcgggagg acgatgaaat caatgcctgg
     1261 atatacgagg ccaatcgcca tcggcctttg gcagaggtcc tcagcgagtc gcggcaagtc
     1321 ttccagcaac tggtcgagac cctcgatgcc ttttctgagg atgagttcca gaatctgcgg
     1381 cgcatcctcg ggctggaaac agagcaggtc agcggcagca tgttcttcgc tcatttccac
     1441 caggagcacg agccagatat gcgcgtctgg ctggagaagg tgaaaaaagc agggtgactc
     1501 ctctggaatc gagctttttt ggtggtcaaa aggcgttata cgaccggaga tgaacgcctc
     1561 cacacacacg aggaaaggat agtaccatgg gtgacacaca ctttgacccg gcggccttca
     1621 agacacagca gcgcgagcag tggagcaacg tggcacaggg ctggcgaa
//
LOCUS       DASZAK010000362         4169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_648573, whole genome shotgun sequence.
ACCESSION   DASZAK010000362 DASZAK010000000
VERSION     DASZAK010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648573"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VGT44_06365"
     CDS             <1..1156
                     /locus_tag="VGT44_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580459.1"
                     /translation="YNLFVTKDSEGLPKFARAWKAGPPGNQVYLGYAGRGRWGWQGVG
                     AAEGLLATVQLLERHLGVPVEWSSGHMGTALLKLLWQKKPGMILDVARDLRDLPFGLA
                     ARDILFRRALTTAMIGMTLHVYDKNSAYLSACRGLARMGVGEPSHLVAREGGEPIRPG
                     LPGIYRVTLRDAGWRDACFDGELLPPLLEEGQEWITNDLLLYVLGRGMPLHLHEAWVF
                     RDYAPILDKWAKTLWDARARLRLAMATFPQRQAERDAYATVGEIAHVSVGGFAASKEV
                     HPSRRMIHPNWWADVVSKARINQLCNLESLAAGAGAPVLVYSDGVYYASRDPNPHSAV
                     PGILDRQAELGGYKHVYSLPISAALVWASEGLTPSELVALFRQEAARHES"
     gene            1146..1691
                     /locus_tag="VGT44_06370"
     CDS             1146..1691
                     /locus_tag="VGT44_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580460.1"
                     /translation="MNREQANARLQEVVASKFVPVYASSKAEVIASYRERYEGGRGAA
                     GWKQHLIKDLAAQTGMKPKNLEKRFDPARIDNAPRRASEKAQYATLGKEKLPPIGQKL
                     PGNQITITVRAQQAKDFVGGKPVGWRDRAWTATFSGPDAYAFAGAPSFRDVWRKHDYP
                     KDVIDLLDGDGDYGAEITAVV"
     gene            1718..1951
                     /locus_tag="VGT44_06375"
     CDS             1718..1951
                     /locus_tag="VGT44_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580461.1"
                     /translation="MWDVLMKLTLALGALDLLIGLIGVTLSLVFLRRLKQWVRLVRSY
                     YLLSRKFGPPAAGVAGSHVRKKGASPDGHAAMD"
     gene            1929..2075
                     /locus_tag="VGT44_06380"
     CDS             1929..2075
                     /locus_tag="VGT44_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580462.1"
                     /translation="MVTQPWIDALAGLMVGVLFWNLLFVICITIENQELKKKMGDLPK
                     DELP"
     gene            2260..2550
                     /locus_tag="VGT44_06385"
     CDS             2260..2550
                     /locus_tag="VGT44_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580463.1"
                     /translation="MARFSCRAALPPLRYPNIPRLEALYMQVLNQGTHPTLGPFLHAL
                     LPQVEEGVTQFLTAIGGKATIHKITSAGEVLSTDLNLPILRDILQVVDGLAQ"
     gene            2574..3161
                     /locus_tag="VGT44_06390"
     CDS             2574..3161
                     /locus_tag="VGT44_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580464.1"
                     /translation="MAILGPGSVSAPSNHDTGEATPHYQEFPPVTIGGPTGDYSIVCP
                     VTSGRWAEFAIDSIVNGDGGTGALVISGRNPPKALAYDGSVKIDGTNDSVFLYATPIR
                     IPATTTLHPNSAFERITSSEKRVYMRIDAAASTSIYVTLRFRLRILERIPAPAATVHP
                     DHAHNMNKERARATNERLQAMGIPGYAKDGSKDRD"
     gene            3154..3498
                     /locus_tag="VGT44_06395"
     CDS             3154..3498
                     /locus_tag="VGT44_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580465.1"
                     /translation="MTDQADENSAPTEEATQAAAAAETTAQHVEQATQEVAQAAKSAP
                     DAAIEGLLTEVKETLHGVQAELKRANDRAEAATSQPEAQAAPAAPAVAVETPSASMRR
                     VLRNGRWVSRHG"
     gene            3491..>4169
                     /locus_tag="VGT44_06400"
     CDS             3491..>4169
                     /locus_tag="VGT44_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580466.1"
                     /translation="MARTNAERARAYRENKKRREAEAAALLAQGLQPPPEAADEGAIQ
                     VTVNAPSAQQQATTLTLKERLFGGPHKVQVPSPGGGKPAPARKTEGAGGGKKLALVSS
                     AMPTILAGVLVANVRDRLPEDYQPCAPTRQEASAMLTPLFEELARYVEVTGRASETVV
                     NLINVLVAMLAYGARSYVTYVEIKHANRKKILVEGSLAAASASAAAPLSAAGRGDEAA
                     AAGASGVD"
BASE COUNT          770 a         1348 c         1366 g          685 t
ORIGIN      
        1 ctacaacctt tttgtgacga aggatagtga gggcctgccc aagttcgcgc gcgcctggaa
       61 agccgggccg cccggcaacc aggtctacct gggctatgcc gggcgtgggc gctggggctg
      121 gcagggcgtg ggcgccgctg agggcctgct ggcgacggtg cagctgcttg agcgccatct
      181 gggcgttccg gtggagtggt catctggcca catggggact gcgctgctca agctgttgtg
      241 gcaaaagaag cctggcatga tactggacgt ggcgcgcgat ctgcgcgatc tgccctttgg
      301 cctggcggcg cgtgatatac tctttcgccg cgcgctcacc acggccatga tcggcatgac
      361 gctgcacgtc tacgataaga atagcgcgta tctctctgcc tgccggggcc tggcgcgcat
      421 gggcgtgggc gagccgagcc acctggtggc tagagagggc ggcgagccga ttcgtccggg
      481 gctgccaggc atctaccgtg tgacgcttcg tgatgcgggc tggcgcgacg cctgcttcga
      541 tggcgagctg ctgccgcccc tcctggagga ggggcaggag tggatcacca acgacctgct
      601 gctctatgtg ctgggccggg gaatgcccct gcacctccac gaggcgtggg tctttcgcga
      661 ttacgcgccc atcctggata aatgggctaa gacgctgtgg gacgcgcgcg cgcgcctgcg
      721 gctcgctatg gctacctttc cgcagcgcca ggccgagcgc gatgcctacg ccacggtggg
      781 cgagatcgcg cacgtgagcg taggcggctt cgctgccagt aaagaggtgc acccctcgcg
      841 ccgcatgatc caccctaact ggtgggccga tgtggtcagc aaggcgcgca tcaaccagct
      901 ctgtaatctg gagtccctgg ctgccggcgc tggtgcgccg gtgctggtct actcggacgg
      961 cgtgtattac gcaagtcgcg accccaatcc gcacagcgct gtacctggca ttctggaccg
     1021 ccaggccgag ctgggcggct acaagcacgt ctacagcctg ccgatcagcg ccgcgctggt
     1081 gtgggcatcc gagggcctta cgccgagtga actggtggcg ctcttccgcc aggaggcggc
     1141 gcgccatgaa tcgtgagcag gccaacgcgc gcctccagga ggtcgtggcc agcaaattcg
     1201 tgcccgtcta cgcctcgtcg aaggccgagg tgatcgccag ctaccgcgag cggtacgagg
     1261 gcgggcgggg cgcggccggg tggaagcagc acctcatcaa agacctggcc gcccagaccg
     1321 gcatgaagcc gaagaacctg gaaaagcgct tcgacccggc gcgtatcgac aacgcgccgc
     1381 ggcgtgccag cgaaaaggcg cagtacgcca cgctcggtaa ggagaagctg ccccctatcg
     1441 gccagaagct gcctggcaac cagatcacga tcacggtgcg cgcgcagcag gccaaagatt
     1501 tcgtgggagg caaaccggtg ggctggcgcg atcgcgcctg gacggccacc tttagcggcc
     1561 cagatgccta cgccttcgcg ggtgcgccga gctttcgcga cgtgtggcgc aagcacgact
     1621 atccgaagga tgtcatcgat ctactcgatg gcgatggcga ttatggcgcg gagatcaccg
     1681 ccgtcgtcta agtgaaagtg aaagggagaa atgagccatg tgggatgtat tgatgaagct
     1741 gacgctggcg ctgggcgcgc tcgatctgct gatcggattg atcggggtca ccctgtcgct
     1801 ggtctttctg cgacgcctca agcagtgggt gcgcctggtg cgtagctact acctgctgtc
     1861 gcgcaagttc gggccacccg ccgccggcgt ggcaggcagc catgtccgca agaaaggagc
     1921 gtcgcccgat ggtcacgcag ccatggattg atgcgctcgc tgggctgatg gtaggagtcc
     1981 tcttttggaa cctgctattc gtgatctgta tcacaattga aaaccaggag ctgaagaaaa
     2041 agatgggaga cctgccgaaa gacgagcttc cctagcgcgt gcaaggcgcg ccccgcgcgg
     2101 ggcgcgccgt tttatttggc gacattcgct atcatgagag tgagcgagcc ataggccaaa
     2161 acctctcctc tgggtacatg tggtaatgcg tctcgctcgg ggcccccctt cgccaaaatt
     2221 tggtaggggc tgtcatactg aaaacgacgc agcgctggca tggcgcgttt ctcatgccgg
     2281 gcggccctgc cgccgctgcg ttaccccaac attccccggc tggaggctct atacatgcaa
     2341 gttctcaatc agggtaccca cccgacgctc ggtccgtttt tgcacgccct acttccccag
     2401 gtggaggagg gcgttacgca attcttgacg gcgattggcg gtaaggccac cattcacaag
     2461 atcacaagcg cgggcgaggt cctctccacc gatctcaacc tgcccattct gcgcgacatc
     2521 ctgcaagtag ttgatggcct ggcgcagtag cctggcctcg cgaggacaca cccatggcga
     2581 ttctcggccc cggctctgtc tccgccccat cgaatcacga tacgggtgaa gcgacgcccc
     2641 actaccagga attcccgccc gtcacgattg gtggccctac cggcgactac agcatcgtct
     2701 gtccggtgac ctcggggcgc tgggcggagt tcgccattga ttccattgtg aatggcgatg
     2761 ggggcaccgg cgcgctcgtc atcagcgggc gcaatccccc gaaggcgctg gcctacgacg
     2821 gctcggtaaa aatcgatggc accaacgatt cggtcttttt gtacgcgacg ccgatccgca
     2881 tcccggccac gaccacgctg catcccaact ccgcctttga gcgcattaca tcaagcgaga
     2941 agcgcgtcta catgcgcatc gatgcggcgg ctagcacctc gatctatgtg acgctgcgct
     3001 tccgcttgcg catcctggag cgcatccccg cgccggcagc gaccgtacat cccgaccacg
     3061 cgcacaacat gaacaaggag cgcgcgcgcg ccaccaacga gcgcctgcag gcgatgggca
     3121 tccccggcta tgccaaagat gggagtaaag accgtgactg accaggctga tgagaacagc
     3181 gcccccacag aagaagcgac gcaagctgcg gccgcggccg agacgactgc gcagcacgtc
     3241 gagcaggcca cccaggaagt cgcccaggcc gccaagagcg ccccggatgc ggccatcgag
     3301 ggactgctca ccgaggtgaa agagaccctg cacggggtgc aggccgaact gaagcgcgcc
     3361 aatgatcgcg cggaggcggc aacgagtcag cctgaagcgc aggccgcccc tgccgccccc
     3421 gctgttgcag tggagacgcc ctccgcgtcc atgcggcgcg tcctccgcaa tgggcgctgg
     3481 gtcagccgcc atggctagaa ccaacgccga gcgcgccagg gcgtatcgcg agaacaaaaa
     3541 gcgccgggag gccgaggccg ctgcgctcct tgcacagggc ctccaaccac cgcccgaagc
     3601 ggcggatgag ggcgcgatcc aggtcacggt caacgcgccc tcggcgcagc agcaggcgac
     3661 cacactcacg ctgaaagagc gcctcttcgg cggcccgcac aaggtgcaag ttccctcgcc
     3721 gggcggcggt aagcccgcac cggcgcgcaa gacagagggc gccggcggcg gcaagaagct
     3781 ggcactggtt tcctcggcta tgcctaccat actggcaggg gtgctggtgg ccaatgtgcg
     3841 cgaccggctg cccgaggact accagccctg cgcgcccacc agacaagagg ccagcgccat
     3901 gctgacgccg ctcttcgagg agctggcgcg ctatgtggag gtcaccggca gggccagcga
     3961 gacggtcgtc aacctgatca atgtcctcgt ggccatgctc gcctacggcg cgcgcagcta
     4021 cgtcacctac gtggagatta aacatgccaa ccgcaagaaa atcctcgtcg aaggcagcct
     4081 cgccgccgcc agcgcttccg cagccgcccc cctctctgcc gcaggacgag gagacgaagc
     4141 ggcggctgct ggagctagtg gagttgacg
//
LOCUS       DASZAK010000363         3623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_258197, whole genome shotgun sequence.
ACCESSION   DASZAK010000363 DASZAK010000000
VERSION     DASZAK010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258197"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1189)
                     /locus_tag="VGT44_06405"
     CDS             complement(44..1189)
                     /locus_tag="VGT44_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEV2580467.1"
                     /translation="MATALVYDPIFLEHITPPNHPEQPERLRRAMEVLEALGWLEREG
                     LVLLSPRAASVDELAAAHDRPYIRKVEAAARRAAEKEAAGGRASYFFATDTYVSAKSY
                     EAAVLAAGAPLTAIDAIMEGEVENAYCLVRPPGHHAVAESAMGFCLFNNVAVAARYAI
                     DRYGLERVMIIDFDVHHGNGTQEMFYHDQRVLYLSTHQAPFYPGTGLSDECGEGAGLG
                     TTINIPLPAGTGFETFDPLFRQVVAPAADRFDPQLILVSAGFDSHWDDPLGGMNLSTA
                     GFSKLVGLLIEEAKFLCQGRIVFVQEGGYSIEAMAACVATCINLLLGDDAAVDSLGPG
                     ATLPYRLNTDVLIAELRRIHNLKGYRMRNAPKPDIERLRREVKGPDM"
     gene            complement(1304..2179)
                     /gene="sucD"
                     /locus_tag="VGT44_06410"
     CDS             complement(1304..2179)
                     /gene="sucD"
                     /locus_tag="VGT44_06410"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEV2580468.1"
                     /translation="MSILLDSNTRVIVQGITGREGLFHTRNMKAAGTNIVGGVTPGKG
                     GETVEGLPVFDTVNEARAATGATASCIFVPPPGAADAIMEAASAGIELIVCITEGIPV
                     VDMTRAMLMVREAGARLIGPNCPGMCTPGQGKIGIIPYQIFTPGPVGFISRSGTLTYE
                     VVALLTEAGLGQSTCIGIGGDPIIGSTFADYLQLFEADPGTKAVVMCGEIGGSDEEDA
                     AEYIKTMSKPVISFVSGRTAPPGKRMGHAGAIISGNTGTAQGKVAALQAAGVPVADTI
                     FDIPGLVKEALARIE"
     gene            complement(2190..3329)
                     /gene="sucC"
                     /locus_tag="VGT44_06415"
     CDS             complement(2190..3329)
                     /gene="sucC"
                     /locus_tag="VGT44_06415"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEV2580469.1"
                     /translation="MKIHEYQAKDILARYGIPVQPGRVAYTPEEAEAIARELGGPVVI
                     KAQVYVGGRGKAGGIQFGDTPAEARAAAARVLGMDIKGLTVEKVLVVSKLDIKEEYYL
                     GIILDRKSQAPVVMVSKAGGIDIEEVAATTPEKIIRQPIDMHWGLRAYEARDILARAG
                     LPHNVIGKGGAILAALAKAFIESDASLAEINPLALTADGQVQAADAKILIDDNGLFRQ
                     KEYAAWAETEESNPLEFEAKREGLTYVKLDGDVGIIGNGAGLVMTTLDMVARVGGKPA
                     NFLDIGGGAKAEVMRKALTFVARDPQVKGILVNIFGGITRGEEVAKGVIMAQTVLPAG
                     MPVVVRLSGTGAEEGKALLKDAGLDWGKDMREAAQKIVAAVRGRE"
BASE COUNT          657 a         1171 c         1074 g          721 t
ORIGIN      
        1 ggcgaccctt gcggtcgcca tgccacgtcg ccacgctaca tgactacata tccggtcctt
       61 taacctcgcg acgcaggcgt tcgatgtcag gtttcggggc gttgcgcatt cgatagcctt
      121 tcaggttatg gatgcggcgc agctcggcga tcagcacgtc ggtgttgagg cgatagggca
      181 gcgtggcgcc tggccccagg ctatcgaccg ccgcgtcgtc gccgagcagc aagttgatgc
      241 atgttgcgac gcaggccgcc atggcctcga tgctgtatcc gccttcctgc acaaacacga
      301 tgcgtccctg gcagagaaac ttcgcttcct cgattaatag cccgaccaat ttcgagaaac
      361 ccgccgttga caggttcatg ccccccagcg ggtcgtccca atgagaatca aagcctgccg
      421 aaaccaggat caactgcggg tcaaagcggt cggcagccgg ggccaccacc tggcgaaaga
      481 gaggatcgaa ggtttcaaaa cccgtcccgg ctggcaaggg gatgttgatg gtcgttccca
      541 gacccgctcc ctcaccgcat tcatctgaga ggccggtgcc tggataaaag ggcgcctggt
      601 gcgtcgagag gtagaggacg cgctggtcgt ggtagaacat ctcctgcgtg ccgttgccat
      661 gatgcacgtc gaagtcgata atcatgacac gctcaagccc gtagcggtcg atggcgtagc
      721 gcgcggcgac cgctacgttg ttgaagaggc agaagcccat ggccgattcc gcgacagcat
      781 gatgacctgg cgggcgcacg aggcagtagg cgttctcgac ttcgccctcc ataatcgcgt
      841 cgatggcggt gagcggcgcg cccgccgcca ggacagccgc ctcatacgat tttgccgaaa
      901 cataggtatc ggtggcgaag aagtaggagg cgcgcccgcc tgcagcttcc ttctcagcag
      961 ccctgcgcgc cgccgcctca actttgcgga tgtaggggcg gtcatgcgcc gccgcaagct
     1021 catccacgct ggcagcgcgc ggcgagagca gcacgagtcc ctcgcgctcc agccatccca
     1081 gggcttccag cacctccatc gccctgcgaa ggcgttcggg ctgctctgga tggttgggcg
     1141 gcgtaatatg ctctaaaaaa attggatcat agaccagggc cgttgccata gattgatgcg
     1201 ccttatcacg aatattgtcg tagggacagg cacaaggccc tcgccgctcc tctccccgct
     1261 tccctgtccc tactgattat atacatattt gtacaccctc cggttactcg atacgtgcca
     1321 gcgcctcttt caccaggcca ggaatgtcga aaatcgtgtc ggcgacgggt actccggcgg
     1381 cttgcaaggc cgcgactttg ccctgcgcgg tgccggtgtt gccggagatg atcgccccgg
     1441 cgtgacccat gcgcttaccc ggaggagcgg tacgcccgga gacgaacgaa atgactggct
     1501 tgctcatcgt cttgatatac tcggcggcat cttcctcgtc gctaccgccg atttcgccgc
     1561 acatcacgac cgccttcgtg ccgggatcgg cctcgaacag ttgcaggtaa tcggcgaagg
     1621 ttgagccgat gatcgggtcg ccgccgatgc cgatgcacgt ggattggccg aggccagcct
     1681 cggtcagcag cgccacgact tcgtaggtca acgtgccgga gcgcgaaatg aagccgacgg
     1741 ggccgggtgt gaagatctga tacgggataa tgccgatctt gccctggccg ggagtacaca
     1801 taccggggca gttggggccg atcagccgcg ccccggcttc gcgcaccatc agcatggcgc
     1861 gcgtcatgtc cacgaccggg atgccctctg tgatgcagac gatcagctcg atgcccgcgc
     1921 tggctgcctc catgatggcg tcagccgcgc cagggggcgg cacgaagatg cacgaggccg
     1981 tcgcgccggt agcagccctg gcctcattta ctgtatcgaa gacgggcaga ccctcgacgg
     2041 tttctccccc cttgccgggc gtgacgccgc cgacaatgtt cgtgcctgcc gccttcatgt
     2101 tgcgcgtgtg gaacaggccc tcgcgcccgg tgatgccctg cacgattacg cgcgtgttgc
     2161 tatccaacaa aatactcatt gcgtttcctc tattccctgc cgcgaactgc cgcgacaatc
     2221 ttctgggcag cttcgcgcat atctttaccc cagtccagcc cggcgtcctt cagcagcgcc
     2281 ttgccttctt ccgcgcctgt gccggacagt cgcacgacaa ccggcatccc tgccggcagc
     2341 acggtctgcg ccatgatgac gcctttcgcc acttcctcgc cgcgtgtgat gccgccgaaa
     2401 atattgacca ggatgccctt gacctgcggg tcgcgcgcga cgaaggtgag ggctttgcgc
     2461 atcacttcgg ccttcgcgcc gccgccgata tcgaggaagt tggccggttt gcctccgacg
     2521 cgcgccacca tgtcgagcgt ggtcatgacc aggcccgcgc cgttgccgat gataccgacg
     2581 tcgccgtcaa gtttgacgta ggtcagaccc tcgcgcttcg cctcaaattc gaggggattc
     2641 gactcctccg tctccgccca tgctgcgtac tctttctggc ggaacaggcc gttgtcgtcg
     2701 atcagaatct tggcgtcggc ggcctgcacc tgcccgtcgg cggtgagagc cagcgggttg
     2761 atctcggcaa ggctggcgtc gctctcaatg aaggctttcg ccagggcagc caggatcgcg
     2821 ccgcctttgc caatcacgtt gtggggcagc ccggcgcgcg ccaggatgtc gcgggcttcg
     2881 taggcgcgca atccccagtg catatcgata ggctgccgga tgatcttttc tggggtggtc
     2941 gccgccactt cttcgatgtc gatgccgccc gctttcgaga ccatcacgac cggggcctgc
     3001 gacttacggt cgaggataat gccgagatag tattcctctt tgatatccag cttcgagacc
     3061 accagcacct tctcaacagt cagccccttg atatccatgc ccagcacgcg agccgccgcc
     3121 gcgcgcgcct ccgcgggcgt atccccaaac tgaatgccgc ccgccttacc gcgcccgccg
     3181 acatacacct gcgccttgat aaccaccggt ccgccgagtt cgcgggcaat cgcctcggcc
     3241 tcctccggcg tatacgccac gcgccccggt tgcaccggga tgccatagcg cgccaggata
     3301 tccttggcct gatattcgtg gattttcaca gaaccgatac tccttttgct ccgtcttcgc
     3361 gagtgaccca tgaaataaaa accgccgcaa cgaggcggtg caagctgtct tgtaatccaa
     3421 cgtcatgcta gcacgtcaat cggtggagtg tcaagaaatg ttggccgccg tcgtatagac
     3481 gcgatacatc acgtccgggt ggcaatgctc gaccagggac gaggacgcga tgatgtcttt
     3541 taacaaacaa ggtcggtaat cctccgtagt acatagtcaa ggttgatttg gcatgtaggg
     3601 gcgcaattca ttgcgccctc gga
//
LOCUS       DASZAK010000364         2210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_364657, whole genome shotgun sequence.
ACCESSION   DASZAK010000364 DASZAK010000000
VERSION     DASZAK010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364657"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            441..1472
                     /locus_tag="VGT44_06420"
     CDS             441..1472
                     /locus_tag="VGT44_06420"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2580470.1"
                     /translation="MVKKAAQTSTRGIPIRERAFAMSNIIGVNAYHVRLDALKRRLIE
                     GGYDSHETPHFLVCCRPEPSTAPGASMLLVHWFAPDAIDSNLGHYLMEELKPLGLLAN
                     AQQYGEVIGAVVNSVAPRNPEQAWHLFATNTLRGLRDLIASEQPAPDWSFPMAVFAAL
                     YRRVYDLCAGESLLDAGCSSGFLPLMMAELLPSLTHVVGIDIYADPFAVARMIAREKH
                     LVNVQFIQADLLSDDMALLPAFDTVTLLHVLEHFSDSAIYGVLANLLKLTARRLIIAV
                     PFEEGEPEPAYGHEQLFTREKLEAVGNWCIEQWGNGTMRYEDCAGGLLWVERMGRDQT
                     HRVFPALPG"
     gene            complement(1557..1850)
                     /locus_tag="VGT44_06425"
     CDS             complement(1557..1850)
                     /locus_tag="VGT44_06425"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEV2580471.1"
                     /translation="MPTYISLVNLTEQGIREVKNAPERLKFFDEAAKEAGARLIGFYL
                     VMGQYDYVIITEAPDDQTAARLILGSIAQGSIRTQTMRAFPRDEFESIARGMH"
     gene            complement(1992..>2210)
                     /locus_tag="VGT44_06430"
     CDS             complement(1992..>2210)
                     /locus_tag="VGT44_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580472.1"
                     /translation="HSSPIRTLPAFVAITWFSRPYFGAILAALAYFLLNSGLFLLSVV
                     PSQRYASYAVVAAIAGMCEGRIFFRQKP"
BASE COUNT          485 a          557 c          669 g          499 t
ORIGIN      
        1 gtaatgctcg gcggtgctga cgctggggaa gtccgtgtca tggtgggtaa agctgggagc
       61 aagtagctcg tcgaagacag ccagcttttt ctgattccag ccctgatcca cgaaatgacg
      121 aacaagcgcc ttgttgtttt cggtggacat gagataatct cctttcgggt gttacgtcct
      181 ttctacctgt tgagtttgcg agagacgcga gagtcaggat gtgcagaaac agtaacaaac
      241 acgacaatat gtgacaggga ggagccttac cagggagtaa ctatatacga aaaatagcag
      301 aaatacaaca aacgtgctgt gtagaaaggg gcggattttg tgctgtgtca gggcgattgt
      361 gatatactgc ttttgatcta gctgagcttg tgcagccaca tttggcctgt tgatatgttc
      421 gcgagaattg cggagtgtgc atggtcaaga aagcagcgca gacctcgacc aggggcatac
      481 ctattcggga acgcgccttt gcgatgagca acataatcgg agtgaatgct tatcatgtca
      541 ggctggacgc gctgaaacgc aggctcatcg agggcgggta tgatagccac gaaacgccgc
      601 attttctggt atgctgccga cctgagcctt ccactgctcc cggcgcttcg atgctgctgg
      661 ttcactggtt tgctccagat gctatcgaca gcaacctggg ccactacctg atggaggaac
      721 tcaagccgtt ggggttgctg gcgaatgcgc agcagtacgg cgaggtcatt ggggcggtgg
      781 tcaattcggt cgctccgcgc aatcctgagc aggcgtggca tcttttcgcc acgaatacgt
      841 tacgaggtct gcgcgacttg atagcgagcg agcagcccgc gccagactgg agcttcccga
      901 tggctgtctt cgctgccctc tatcggcgcg tgtatgattt gtgcgctggg gaaagcctgc
      961 tcgatgctgg ctgttcctcc ggcttccttc cactcatgat ggcagagcta cttccatcgc
     1021 tcacacatgt ggtagggatt gatatctacg ccgacccgtt cgcggttgcc aggatgatag
     1081 cgcgcgagaa gcacctggtg aatgtgcagt ttatccaggc agacctgctc tcggatgata
     1141 tggccctgtt gcctgcgttc gatacggtca cgctgctgca tgtgctggag catttcagcg
     1201 attcggccat ctatggtgtg ctggcgaatc tgctcaagct gaccgcgcgc cgcctcatca
     1261 tcgctgtgcc attcgaggag ggcgagccag agccagccta cggccacgag cagctattca
     1321 cacgcgagaa gcttgaagcc gtagggaact ggtgcattga gcaatggggc aatgggacga
     1381 tgcggtacga ggattgtgct ggaggattgc tgtgggtaga gcgaatggga agagatcaga
     1441 cacaccgagt ttttcctgcg ctgcctggat gaagcagcgc aggccagggt tgacctgcgc
     1501 tactcgaccg gctatcgcaa gatgttcgac gtggagacgt gctatttacg ccttgctcaa
     1561 tgcatcccgc gcgcgatact ctcaaactcg tcgcgaggaa aggcgcgcat cgtttgtgtg
     1621 cggatactcc cctgtgcgat gcttcccaga atgagtcgag cggcagtctg atcgtcaggc
     1681 gcctcggtga tgatgacata gtcgtactgt cccatcacaa gatagaagcc tatcagccgc
     1741 gcgccagctt ccttcgcggc ttcatcgaag aatttcaggc gttcgggggc atttttgact
     1801 tccctgattc cttgttcggt gaggttgacc aggctaatgt aggttggcat cagcaatcct
     1861 cctacttggt ggcggtaccc gcaaggatac caggacggag ataatcaagt cgatagagtg
     1921 atctacattc gtaaaacacg ctcagtacct tcattgtaca cgcacgatgc aactcataca
     1981 agtggaggtc gtcaaggctt ttgccggaaa aagatgcgtc cctcgcacat acccgcgatt
     2041 gccgcgacga cggcatagct ggcgtagcgc tgcgaaggca cgacgcttag caggaacagg
     2101 ccgctattga gcaagaagta ggccagggca gccaggatcg cgccgaaata gggacgcgag
     2161 aaccatgtga tggcgacaaa ggcggggagc gttctgattg gcgaggaatg
//
LOCUS       DASZAK010000365         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_364700, whole genome shotgun sequence.
ACCESSION   DASZAK010000365 DASZAK010000000
VERSION     DASZAK010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364700"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VGT44_06435"
     CDS             complement(<1..317)
                     /locus_tag="VGT44_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine--fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEV2580473.1"
                     /translation="MVESLMAAEMAEQPVVLARLIERFGNDVARVRAVLPERLAGIVF
                     VARGSSDHAAVFGRYLAELAAGRPAGLAAPSLHTLYAAHVDYSGYLAVALSQSGATPE
                     IIT"
     gene            complement(355..1941)
                     /locus_tag="VGT44_06440"
     CDS             complement(355..1941)
                     /locus_tag="VGT44_06440"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEV2580474.1"
                     /translation="MKFPLFLPQRKWGYVLYVGKVFLIAAIYFSTARFGLSLDAVSGF
                     ATLVWLPSGIALAAGLLFGYRLWPGIFIGAFLANLLNGAPIFVAIGIGIGNTLEPMVG
                     TYLLKSNRFSSKLNQLSDVLLLVLLAMPLSALISATVGVSSLLLGKVIIFSAFFTTWS
                     AWWIGDMISILILTPFLLIWSKLSYETVAVKRYVEIGLLTISLLLTGLVVFLGLFHTN
                     RGGFPRTYLVFPPLIWTTLRFGQRGTLTALLGLSILAIIGTIQGLSPFSGGNPGISLI
                     ALQSFMGVTAITSMILAAEVEERKELERRKDDFISMASHELKTPLTSLLGYTELLHRK
                     AAEPGSQEAQRYLAKMTAQIEKLSRLIADLLDISKIQAGKIVYAEEAVDVDELVREVV
                     ETLQQSNSKHHISIEGRAQREVVGDRERLEQVLINLISNAIKYSPQAEKVIVRLTHLS
                     NSLTISVQDFGIGIPMTHQKKVFERFYRVYNDKDRTYPGLGIGLYIAYQIIEQHHGKM
                     WVESVEEQGSTFSFSLPLRN"
     gene            complement(2063..>2751)
                     /locus_tag="VGT44_06445"
     CDS             complement(2063..>2751)
                     /locus_tag="VGT44_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2580475.1"
                     /translation="LLPNPFALSSYAVILSNSLFLRWMLNAFIYAAGAMVLNVLFSAM
                     AGYALSRLNFRGREFIFLITLGIMMIPSAVTLIPKFLVVDNLHINNTYFALILPAMAQ
                     PFSVFIMVQFMKGLPKELEESANIDGASRFRTFFQIILPQVKPALTAVAIITFQGAWN
                     DFQWPLVALGTQDMYTLPLGLFYFKSAHYTEYNLLLAGSMFNTIPILILFFIFQRYFI
                     EGAVSSGVKG"
BASE COUNT          695 a          709 c          769 g          578 t
ORIGIN      
        1 atcgtgatga tctctggcgt cgcgccggac tggctcaacg cgactgccag gtagccgctg
       61 taatcgacat gcgcggcgta cagcgtatgc aggctgggag cggcaaggcc agccgggcgg
      121 ccagccgcca gctcggcgag gtagcgaccg aagaccgccg cgtggtcaga agatccccgc
      181 gcgacgaaga caatccccgc caaccgctct ggcaggactg cgcgaacgcg agccacgtca
      241 ttgccaaagc gctcgatcag ccgggcaagc actaccggct gctccgccat ctcggcagcc
      301 atcaacgatt cgaccatttc caactctcct cttgatagtg gtctgacgat cagtttagtt
      361 ccgtaaaggc agcgagaagg agaaggtcga gccttgctct tcaacgcttt ctacccacat
      421 cttaccatga tgctgctcaa tgatctggta cgcgatatac aggccaattc ccaggccggg
      481 atacgttcga tctttgtcat tatagacacg gtagaagcgc tcgaagacct tcttttgatg
      541 cgtcataggt atgccgatac cgaaatcctg gacactgatc gtcaggctat tggacagatg
      601 ggtgaggcgc acaatgacct tctctgcctg tggagagtat ttaatcgcat ttgatatcag
      661 gttgatcaat acctgttcaa gacgctccct gtcgccgacc acctcgcgct gcgctcttcc
      721 ctcgatggaa atgtgatgct tcgagttgct ctgttgcaag gtctcaacaa cttcgcgaac
      781 gagttcatcc acgtcgacag cctcctccgc gtagacgatt ttacctgctt gaatcttaga
      841 aatgtcgagc aggtcggcaa tcagcctgga aagtttctcg atctgcgccg tcatctttgc
      901 aaggtagcgc tgtgcttcct ggctcccagg ctcggcagcc ttcctgtgaa gcagttccgt
      961 atagcccagc aggctggtca gaggggtctt caactcgtga cttgccatgc tgataaaatc
     1021 gtctttgcgc cgttcgagtt ctttgcgttc ctccacctct gccgctagaa tcatggaggt
     1081 gatcgccgtt actcccataa agctctgcaa ggcgatcaga ctgatgcctg gattgcctcc
     1141 tgaaaaaggg gagaggccct gtattgtgcc gataatggca agaattgata aacccagaag
     1201 agcagtgagg gttccacgtt ggccgaatcg tagggtggtc cagatgagtg gagggaacac
     1261 aaggtatgtg cgtgggaaac ctccccggtt cgtgtggaag aggccgagaa agacgaccag
     1321 gccggttagc aggagggaga tggtaagcag cccgatctca acgtatcgtt taacagctac
     1381 tgtttcatag gacaatttgc tccaaatcag gagaaaaggg gtgagaatga ggatactgat
     1441 catatctcca atccaccacg cactccaggt agtgaaaaag gctgaaaaaa taatgacctt
     1501 ccccagcagt agactggaca caccaaccgt ggcgctgatc agagcgctca ggggcatggc
     1561 aagcagaacc agcaaaagaa cgtcactgag ctggttgagt ttactactga atcgattgct
     1621 tttgagcagg taggtaccga ccatcggctc aagcgtattg ccgatgccga tgccgatggc
     1681 aacgaagatg ggagcgccat tgagcagatt ggctagaaat gctccaatga aaatgcctgg
     1741 ccacaggcgg tagccaaaga gcagccctgc ggcgagggcg atacctgagg gaagccagac
     1801 gagcgtagcg aatccactga ccgcgtccag ggaaaggccg aatctcgctg tgctgaaata
     1861 aatggctgca atcaggaaga ccttgcctac atagaggaca tatccccatt ttcgctgagg
     1921 taggaacaac gggaacttca tctatggcac ggcctcccta caacttgcta actcatctac
     1981 ccgactgatt ctctaactat gaagatgcat cgacttttct gtagcccaag agctattata
     2041 gtccatcagt caagacgaat gcttatccct ttacgccgga agacactgcg ccttcgatga
     2101 agtagcgctg gaagatgaag aagaggatca ggatgggtat ggtattgaac atcgaaccgg
     2161 ccagcaacag gttgtactcc gtatagtggg ccgatttgaa gtaaaacaag cccagaggca
     2221 aagtgtacat gtcctgtgtt cccaaagcga ccaggggcca ctggaagtcg ttccaggcgc
     2281 cctggaaggt gataatggca acggcagtca gggccggttt aacctggggg agaatgatct
     2341 ggaagaaagt acggaagcgc gatgcgccat cgatattggc cgattcttcc agttctttcg
     2401 gcagcccctt catgaattgc accatgataa acaccgagaa gggctgcgcc atggccggca
     2461 ggatcagcgc gaagtaggtg ttgttgatat gcaggttatc gaccaccaga aatttaggaa
     2521 tgagcgtcac cgccgaagga atcatcatga tgcccagtgt aatcaggaag atgaattctc
     2581 tgccgcgaaa atttaaacgc gacagggcgt agccggccat cgcggagaag aggacattga
     2641 gaaccatcgc cccggctgcg tagatgaagg cgttgagcat ccagcgcagg aagagggaat
     2701 tgctcaggat taccgcataa ctgctcaggg cgaagggatt gggaagcagc g
//
LOCUS       DASZAK010000366         5271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_541481, whole genome shotgun sequence.
ACCESSION   DASZAK010000366 DASZAK010000000
VERSION     DASZAK010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5271
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541481"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="VGT44_06450"
     CDS             complement(<1..397)
                     /locus_tag="VGT44_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580476.1"
                     /translation="MTTQPTWEELAATAITYLREKDPVMALAIERVGPCTLVPNPHFF
                     ETLVDAIISQQISVKAADAIMGRLRRAALGEAGGLVTPESLLRLEEDALRAAGLSPQK
                     IRYMRDLTERITDGRLDLARLSELEDEAVI"
     gene            complement(638..1489)
                     /locus_tag="VGT44_06455"
     CDS             complement(638..1489)
                     /locus_tag="VGT44_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2580477.1"
                     /translation="MATTTTVKSVPVAKKRAQGLSVTMRAMTNSSTPLIVACFYGLLI
                     LLILAPIYPVMAQANFQALMNSSIMSAVLGGHLTSLFNFSALLAVEVFSSIYGLLFGG
                     FVAWIGGAVLPVTIEDGTLDLALSRPINRTRYYLESWFAVLIGALIISLVLALGVWID
                     TFIVTNPGIDWTWMWITLLVQWTFLFFAAGLGMLCGSFINASRAAGGTALGIVVLGYL
                     INTFGGLTNQFQWVLKISPFYYAPAIDPLINHQLTWWYPCVLLAAGLVFGIAGLVIFN
                     KRDLPTV"
     gene            complement(1520..2434)
                     /locus_tag="VGT44_06460"
     CDS             complement(1520..2434)
                     /locus_tag="VGT44_06460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580478.1"
                     /translation="MISEEPVIVIDHLHKSYGKVQAVKGISMNVRRGEIFGFLGPNGA
                     GKTTTIRCMLDVIRPTSGTLRVLGLDAQRDGHALHQHIGYLPGDVRLPGRMTGKQVIN
                     YFANLQGREPVLLNDLVERFDVEMKRPLKGYSKGMRQKIGIVLAFMCDPDVLILDEPS
                     SGLDPLLQKVFNEFLLEEQARGKTIFMSSHIMSDVEKVCQRVAVIRSGELVTVEEVGA
                     LREKAGQHIIVEFGDAVSEQELARIPGVSALIKTNSSYHFTTIGSMDVLIKALSQHEV
                     IRLTSQEAPLEEVFLKFYEEPEKAAPVA"
     gene            complement(2431..3165)
                     /locus_tag="VGT44_06465"
     CDS             complement(2431..3165)
                     /locus_tag="VGT44_06465"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2580479.1"
                     /translation="MAARRASAEPAMEERTKRQERAGRILDAAAELTLRWGYNKTTID
                     DIARQAGVAKGTIYLHWKTREELFRALLAREAVFLAEEMQPRMANDPEGMTIHGMMKH
                     SVLAISKRPLMKAVILRDSELLGAWERYEYSQERTQLRLDTYSALLRQLRERGLVRTD
                     LSERQENYILSFLGIGILMVDPWLPDDFKLSDEEAADMLAETVRRTLEPRDAPVNDAQ
                     AARSLAEYMDFEVQVAKKRNQEEMES"
     gene            3528..3767
                     /locus_tag="VGT44_06470"
     CDS             3528..3767
                     /locus_tag="VGT44_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580480.1"
                     /translation="MPRTTPVAIVELNGQRLLSGAFGEVDWVRNLRAAKEATLQRGRR
                     PEHIVVDELSPVSAVNRLLLVRQGSPLRVGLLTHG"
     gene            3816..>5271
                     /locus_tag="VGT44_06475"
     CDS             3816..>5271
                     /locus_tag="VGT44_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEV2580481.1"
                     /translation="MSDVTHTSTESEHTHTNRLIHETSPYLLQHAHNPVDWYAWGEEA
                     LQRARELDRPILLSVGYSACHWCHVMERESFENEEIAALMNEHFVSIKVDREERPDID
                     NIYMQAVQAMTQQGGWPMTVFLTPGGRPFYGGTYFPPRDRQYGREMMPGFRTVLLTIA
                     DIYQNQREKVEEQAGQLADYLRKRSASPLRSRGDAASTGAMPLELLASASRELAADFD
                     AANGGFGHAPKFPNTMSLEFLLRMHLHRIRGEIGTKATRPELEIVETSLQRMAEGGIY
                     DQLGGGFHRYSVDAEWLLPHFEKMLYDNALLSRIYLHTYLVTGKPLYRRIAEETLDYV
                     AREMTSPEGGFYSTQDADSEGEEGKFYTWTPAEVEAALPAEDAALFMRYYDITTEGNF
                     EGKNIAHIERDLRDIAEEAGISLEQLQEALQRGRQTLFTLREARVHPGRDEKILTAWN
                     GLMLRSFAEAARHLDRADYLRLATRNADFLLEKLA"
BASE COUNT         1136 a         1511 c         1491 g         1133 t
ORIGIN      
        1 cgatgacggc ctcgtcttcc aactcgctca accgcgccag gtcgagccga ccatcggtga
       61 tgcgctccgt caaatcgcgc atgtaacgta tcttctgcgg cgaaagtccc gctgcccgca
      121 gcgcgtcttc ctccaaccgc agcagcgact ccggcgtcac cagcccgccc gcttcgccga
      181 gcgcggccct gcgcaggcgt cccataatgg cgtccgcggc tttgaccgag atttgctgcg
      241 aaatgatggc gtccaccagc gtctcgaaga aatgggggtt aggcacaagc gtgcagggtc
      301 ccactcgttc aatcgccagc gccataacag ggtctttttc gcgcaggtag gtgatggcgg
      361 tagcagcaag ttcttcccag gttggttgcg ttgtcattgg tattccttta caaatgaaat
      421 gcggtagaat ttagaacatt ctaccgcata tatcatgtaa aagctagagg aggcactcag
      481 cccgccgtag gagccggttt accgcgctca ccgccgatta tgaattttac aacccacatc
      541 ggtgctgtca tcctgagcgc agcgaaggat ctcggtcctc gccctgtggg atcctgagcg
      601 cagcgaagga tgacagtgta ttgtctgtcc tggtccttta caccgtcggc aggtcgcgct
      661 tgttgaagat caccaggccg gcgataccga acaccaggcc ggctgccagc aggacgcagg
      721 gataccacca ggtcagctga tggttgatga gcgggtcgat ggccggcgca taatagaagg
      781 ggctgatttt gagtacccat tggaactggt tggtgaggcc gccaaaggtg ttgatcaggt
      841 agcccagcac aacgatgccc agcgctgttc ccccggctgc ccggctggca ttgatgaagg
      901 agccgcagag catacccaga cccgctgcga agaagaggaa ggtccattga acaagcaggg
      961 tgatccacat ccacgtccag tcgatcccag ggttggtgac aataaatgtg tcaatccaga
     1021 cccctaaggc aagcaccagg ctgatgatca gcgcgccaat caagacggca aaccagcttt
     1081 caaggtagta acgcgtgcgg ttgatcgggc gagaaagcgc caggtcaagc gtgccatcct
     1141 cgatggtaac aggcagaacc gcgcctccta tccaggccac gaagccgccg aagagcaggc
     1201 catagatgga actgaaaacc tcaacggcca ggagcgccga gaaattgaac aagctggtca
     1261 ggtgcccgcc gagtacggct gacatgatac ttgagttcat tagggcctga aagttagcct
     1321 gggccatcac cgggtagatc ggagccagaa tgagtaggat caaaagccca taaaaacatg
     1381 ctacgatgag tggcgtgctg ctgttggtca tcgcccgcat ggtcacgctc aggccctgcg
     1441 cgcgtttctt cgcgactgga acgcttttga ctgttgtcgt tgtcgccatg atcatatctc
     1501 cttgttgagc cacggcccgt tatgccacgg gagcggcctt ttccggctct tcataaaact
     1561 tcaagaatac ctcttccagc ggcgcttcct gagaagtcag gcggatcacc tcgtgctggc
     1621 tgagcgcttt aatcaacacg tccatgctac cgatggtggt aaaatgatac gagctattcg
     1681 tttttatcag tgcgctcaca ccaggaatgc gcgccagctc ttgttctgat accgcgtcac
     1741 cgaactcgac tatgatatgc tgtcccgcct tttctcgcaa ggcccccacc tcttcaactg
     1801 ttaccagttc tccgctacgg atcaccgcca cgcgctggca aaccttttcc acgtcgctca
     1861 tgatgtgcga actcatgaag atggttttgc cacgcgcctg ctcctccagc agaaactcgt
     1921 tgaacacttt ttgcaaaagc gggtcgaggc cagaggatgg ctcgtccagg atcagcacgt
     1981 ctggatcgca cataaaggcc agcacaatgc cgatcttctg cctcatacct ttggagtagc
     2041 ctttcaacgg gcgcttcatc tctacgtcaa agcgttcgac cagatcgttg agcagcaccg
     2101 gttcgcggcc ttgcaggttg gcgaagtagt taatgacctg cttgcccgtc atgcgccccg
     2161 gcaggcgtac atcgcccggc aggtagccga tgtgctggtg cagggcgtgt ccatcgcgct
     2221 gcgcgtccag ccccagcacg cgcagtgtcc cggacgtggg gcgaatgaca tccaacatgc
     2281 agcgaatggt ggtggttttg cccgcgccat ttggccccag gaagccaaag atttcgccgc
     2341 gtcgcacatt catcgaaatg cccttgaccg cctgcacctt accgtaggat ttgtgtaagt
     2401 gatcgatgac aataaccggc tcttctgaga tcatgattcc atctcctctt gattacgttt
     2461 tttcgctacc tgtacctcaa agtccatata ttcggccaaa gacctcgcgg cctgcgcgtc
     2521 gttcaccggc gcatcacgtg gttcaagcgt gcgccgcacg gtctcggcca gcatatcagc
     2581 ggcttcctcg tcggagagct tgaagtcatc cggcagccag gggtcaacca tcaggatgcc
     2641 gatgccgaga aagctcagga tatagttttc ctggcgctcg ctcaggtcag tccgtactag
     2701 ccctcgctct cgtaactggc gcagtaacgc cgaatatgta tccagccgta attgcgtgcg
     2761 ctcctggctg tactcgtagc gttcccacgc gcccagcagt tcggagtcgc gcaatatgac
     2821 cgctttcatt aaaggacgct tcgaaatggc cagaactgaa tgtttcatca tgccgtgtat
     2881 agtcatccct tcagggtcgt tcgccattcg cggctgcatc tcttccgcca ggaagaccgc
     2941 ctcgcgcgcg agcaaggcgc gaaagagttc ttcgcgtgtc ttccagtgca ggtagatggt
     3001 tcccttcgcc acacccgcct ggcgcgcgat atcgtcgatg gtggttttgt tgtagcccca
     3061 gcgcagcgtc aactctgccg cggcatcaag aatgcgcccg gctcgctcct gccgtttcgt
     3121 gcgctcctcc atcgctggtt ccgcgctcgc cctgcgtgct gccaatgcga agcctccttt
     3181 ttatgactga atgaccgaat agatacgatg actaaatttg attatctggt cataattgta
     3241 atagatattg ttaataaagt caagagcttg agcgagaaat taatttttaa ctttctaaaa
     3301 aataaattac atcattgtga acaaattgtc aaggatattt aaggaaatta tccaaaattc
     3361 tttagaaaga atatttttaa gaatgaaaag cttgttctct tgacaaagat tgctatagtt
     3421 ttccaacctt tgtcagcgtt agtaaccgtc tgataaagct catgctgcgc gcgggcatga
     3481 aacctggcgc tatggccttg ctcacagtgc ggggctgaaa gagcggattg ccgcgcacga
     3541 cgccggtggc gattgttgag ttaaacggtc agcgtctgtt gtcaggcgct tttggcgagg
     3601 tcgattgggt gcgcaactta cgcgcggcaa aagaggcaac cctgcaacgc ggacgccgtc
     3661 ctgaacacat tgttgtcgat gagttatcgc ccgtgagcgc ggtaaaccgg ctcctactag
     3721 ttcgtcaagg ctcgccccta cgggtcggtt tgctcacaca tgggtgatgt cgtacaatgc
     3781 ctccatagag agacagagca tgatttggag gcgacatgtc cgacgttacg catacatcga
     3841 ctgaatctga acatacacac accaatcgac ttattcacga gaccagcccg tacctgttgc
     3901 agcacgcgca caacccggtg gactggtatg cctggggcga ggaggcgctg cagcgagcaa
     3961 gggagcttga ccggccaatc ctgttgagcg tgggctattc ggcctgtcac tggtgccatg
     4021 tcatggagcg cgagtccttt gagaacgagg agatcgcggc gctcatgaac gagcatttcg
     4081 tttcgattaa ggtggatcgc gaagaacggc ctgatatcga caacatctac atgcaggcag
     4141 tgcaggctat gacccagcag ggcggctggc ctatgacggt cttcctcacg cccggtgggc
     4201 ggccattcta cggtggcacg tacttcccgc cgcgcgaccg gcagtatggg cgcgagatga
     4261 tgccgggctt cagaactgtg ctgctcacca ttgccgacat ctaccagaac cagcgtgaaa
     4321 aggtggaaga gcaggccggg cagcttgccg attacttgcg gaagcgcagc gcttcgcctc
     4381 tgcgtagtag aggggatgcc gcgtcaacgg gagcgatgcc gctggaactg ctggcgtccg
     4441 ccagccgcga actggccgca gatttcgatg ccgccaacgg cggttttggc cacgcgccca
     4501 aattccccaa tacgatgtcg ctggaatttc tgctgcgtat gcacctgcac cgcatacgcg
     4561 gggagatcgg cacgaaggcg acgcggccag agctggagat cgtggagacc agcttgcagc
     4621 gcatggccga aggcggcatc tatgaccagc ttggcggcgg ctttcatcgc tactccgtcg
     4681 atgccgaatg gctcttgccg cacttcgaga agatgctata cgataatgcc ctgctcagcc
     4741 gcatctacct gcatacctat ctcgtgaccg gcaagccgtt gtatcggcga atcgcggagg
     4801 agacgctgga ttatgtcgcg cgcgagatga cctcgccaga gggcgggttt tattccacgc
     4861 aggatgccga cagcgaaggc gaggagggca agttctacac ctggacgccc gccgaggtcg
     4921 aagcggccct cccggcggaa gatgccgcgc ttttcatgcg ctactacgat attaccaccg
     4981 aaggcaactt tgagggaaaa aatatcgcgc atattgagcg agacttgcgc gatatcgccg
     5041 aggaggccgg gatcagcctg gagcaattgc aagaggcgct gcaacgcggg cggcagacgc
     5101 tcttcacgct gcgcgaggcg cgtgtgcatc cggggcgcga cgaaaaaatc ctgacggcgt
     5161 ggaatggcct gatgctgcgc agcttcgccg aggccgcgcg ccatctcgac cgcgccgatt
     5221 acctgcgcct cgccacgcgc aacgctgatt ttctgctgga gaagctggcg c
//
LOCUS       DASZAK010000367         2331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_648736, whole genome shotgun sequence.
ACCESSION   DASZAK010000367 DASZAK010000000
VERSION     DASZAK010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648736"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VGT44_06480"
     CDS             complement(<1..250)
                     /locus_tag="VGT44_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580482.1"
                     /translation="MSTATEQQNWWAEGDTPVRTSTRVTYLVDGHAMMLTLCRHLLMA
                     RRYVYLANWGLAPTIRLVRGEDHRAGPDGSHEQEALLEE"
     gene            complement(398..1801)
                     /locus_tag="VGT44_06485"
     CDS             complement(398..1801)
                     /locus_tag="VGT44_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV2580483.1"
                     /translation="MSILSINPANEEILQTFEPFSAAQVDQALQEARQAFKSWRETSF
                     AERGALFHRMARYLRDHKEELGRLATLEMGKPIVEAEAEAEKCAWNCDFYADNAERYL
                     ADEQVVTNATQSYVEFQPLGVILALMPWNYPYWQVIRFAAPALMAGNTAVLKHASNVS
                     MCALKVEQIFQECGFPPGVFSTVLVPGAETGSLIADRRIAAVTLTGSDIAGVAVASAS
                     GRVLKKNVLELGGSDAFIVLEDADLDGAAQMAVKARFQNTGQSCIAAKRFIVVESVAK
                     AFEQKFVEAASQLKVGDPLQRETQIGPMARGDLRDALEQQVADSVKMGARVLLGGKPL
                     PGKGYFYEPTILTQITSEMPMFNEETFGPAAAVIYAHDAEHAVALANDSDFGLGGNLW
                     TGNIERGRRLARHFESGAVFINSMTASDPRLPFGGIKRSGYGRELSSFGIREFVNVQT
                     VWIGPAIAPQPTKVATE"
     gene            2023..>2331
                     /locus_tag="VGT44_06490"
     CDS             2023..>2331
                     /locus_tag="VGT44_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580484.1"
                     /translation="MYTAERQRAQPYLEVVSGPLYEQWYLLHHRFVRVVTNHLRIAGD
                     IRRFLYYAELMAEQTYERPADLPVNIPEDLMWQAGERLHFPAAFTCYLFETRSDEAFP
                     P"
BASE COUNT          440 a          698 c          686 g          507 t
ORIGIN      
        1 gctcctctaa cagcgcttcc tgctcatgac tgccatccgg ccctgcgcgg tggtcttccc
       61 cgcgcactag cctgatcgta ggggccaggc cccagttggc gagatagacg tagcgacgcg
      121 ccatcaagag atggcggcag agcgtcagca tcatggcgtg tccatccacc aggtacgtca
      181 cgcgggtaga agtacgcaca ggcgtatctc cctccgccca ccagttctgc tgttcggtcg
      241 ctgttgacat atcgatcctc ttcgtcaata ttaatccgtc caggcgcaaa tggggtgtag
      301 gggcgcaatt cattgcgccc tcgtttccgg acgagcggcc tggcaagcgg gcacaatgaa
      361 ttgcgcccct acaccccatt tgcgccctta tagaaatcta ttccgttgca acctttgtcg
      421 gttggggcgc gatggcgggg ccaatccaga ccgtctgcac gttgacgaac tcgcgaatgc
      481 cgaatgaaga gagttcgcgg ccatacccgc tgcgctttat cccaccgaaa ggcaggcgcg
      541 ggtcggaagc ggtcatgctg ttgatgaaga ccgcgcccga ttcgaagtgg cgcgccagcc
      601 tgcgcccgcg ttcgatgttg ccggtccaga ggttgccgcc cagaccgaaa tcggagtcgt
      661 tggccagcgc tacggcgtgt tcggcgtcgt gggcgtagat gacggcggcg gcagggccga
      721 aggtctcttc gttgaacatc ggcatctcgg aggtgatctg cgtcaggatg gtcggttcgt
      781 agaagtagcc cttgccgggt agcggtttgc caccgagcag cacacgcgcg cccatcttca
      841 ccgagtcggc cacctgctgt tccagggcgt cgcgcaggtc gccgcgcgcc atcggcccga
      901 tctgcgtctc gcgctggagc gggtcgccta ccttcaactg gctcgcagcc tcgacaaatt
      961 tctgctcgaa ggctttcgcg accgattcga cgacgataaa gcgtttcgcc gcgatgcaac
     1021 tctggccggt gttttggaaa cgcgctttga cggccatctg cgccgccccg tccaggtcgg
     1081 cgtcttccag cacgataaag gcatccgagc cgcccagttc cagcacattt ttcttgagta
     1141 cccgcccgct tgccgatgct accgcgacgc ccgctatatc gctgccggtc agcgttaccg
     1201 ccgcgatgcg ccggtcggcg atcaggctgc ccgtttctgc cccaggcacc agcacggtgc
     1261 tgaagacgcc cggggggaaa ccgcattcct ggaagatctg ctcgaccttc agggcgcaca
     1321 tcgagacgtt cgaagcatgt ttcagtactg ccgtgttgcc cgccatcagc gcgggtgcgg
     1381 caaagcgaat gacctgccag tatggatagt tccagggcat caacgccagg atgacgccca
     1441 gcggttggaa ctcgacataa ctctgtgtgg cattcgtcac gacctgctcg tcggcaagat
     1501 atcgctcggc attgtcggca taaaagtcgc agttccaggc gcacttctcg gcctcggcct
     1561 cggcctccac aatcggcttg cccatctcca gcgtggccag gcgacccagt tcttctttgt
     1621 ggtcgcgcag atagcgtgcc atgcggtgga acaacgcgcc gcgttcggca aaggaggttt
     1681 ctcgccagct cttgaatgcc tggcgcgcct cctgcaatgc ctggtctacc tgcgccgcgc
     1741 taaacggctc gaaggtttgc agtatctctt cgttggccgg gttaatcgat agtatactca
     1801 tgggatggtc tccttgaaat agggcaatcg tcttctccat ttacgatacg acattgacgg
     1861 ctgtcatggc aaattgagga atgacaatat aagttcgtag ggacaggggc cggtggagtt
     1921 agcccaacct acctattcgt ttagcttgat gctcaagagt gtggtaggat agtcgaacac
     1981 gtatcgtaaa tgctgttagt agcacaaatc aaggggtagc atatgtatac ggctgaacgc
     2041 cagcgagcgc agccatacct ggaggtagtc agcgggccgc tgtatgaaca atggtatctc
     2101 ctgcatcatc gcttcgtgcg cgtcgtgacg aaccacttgc gtatcgccgg ggatatacgg
     2161 cgatttctct attatgcgga gttgatggcg gaacaaacat acgaacggcc tgctgatctg
     2221 ccggtgaata ttcccgaaga tttgatgtgg caggctggcg agcgcctgca tttcccggct
     2281 gcttttacct gctatctctt cgagacacgg tccgatgagg ctttcccgcc a
//
LOCUS       DASZAK010000368         6681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_9223, whole genome shotgun sequence.
ACCESSION   DASZAK010000368 DASZAK010000000
VERSION     DASZAK010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6681
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9223"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VGT44_06495"
     CDS             complement(<1..1115)
                     /locus_tag="VGT44_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEV2580485.1"
                     /translation="MRASFIHIADTHLGYEQYGVRERFNDFSRAFWDITAEAVNRKID
                     FMVIAGDLFHKRAIDALTLIHAIEGLQRLKDRGIPVIAIEGNHDRSYYREGISWLQFL
                     CHQGYLVLLNPRMQNGAPVITAWDQESMEGSHVDVLGGRLRVYGLPWQGATTTRSMEG
                     MALALAAARADEDAAGIEYRLLMMHTGVDGVVPRVQGLPTMSQFQALRNSIDYLALGH
                     VHKPFEFDGWIYNPGSTETVGADEAAWEDRGYYHVEIDTDEQESDEDVGISRHRATHL
                     VSKRRPFVRLEVPVGGASEPQELYDVLDRRCRVERPNFEGDVLRPLIQVHLVGTLTFD
                     AGSLDFPYMEKMVNDLLDPLYVRVENNTNDQDYIPENG"
     gene            complement(1141..1539)
                     /locus_tag="VGT44_06500"
     CDS             complement(1141..1539)
                     /locus_tag="VGT44_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580486.1"
                     /translation="MVDIVSNLHFFNMVLVLASGTITGVWGLILFFMKKQTIIRPWRI
                     SLIFTAVVSLLQALLGIILLLLGQRPGPPGDSLFYLHFVYGGIVALAIPVAVTYATGG
                     KQPRRDLLIYSLISLVLVAAAVRALMTGPS"
     gene            1863..2708
                     /locus_tag="VGT44_06505"
     CDS             1863..2708
                     /locus_tag="VGT44_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580487.1"
                     /translation="MPNHPEMEERNQPPTSGKLVLPSLPDTPGLPGTFPTPRPYTETY
                     NAQITPAQLIRPKGPAAPHGLLARLAFYWRKDPAYKVFMLAVVAVLLASIVFVSMAGA
                     AWLGKPFFGSPYTQSPPTVTAPTGTVDLHPTFGPPAGGTGSGQSSQPPAQSTPALGNT
                     PTPSGSPSPQPSPTQPGQGGTLTVQITGYLPVVPNNSHVPITVTTNEANVTVNLYMQS
                     NGNPPFKRFGPGITDSSGMVTIDWFVSYFSIVKRTITVSITAIGTDQNGQSIRSAPVM
                     VRVYV"
     gene            2730..3689
                     /locus_tag="VGT44_06510"
     CDS             2730..3689
                     /locus_tag="VGT44_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV2580488.1"
                     /translation="MTQTDEIRQVQDAARVVRQNTSKVIVGKEEVIDLLMVALLCEGH
                     VLFEDVPGIGKTTLAKSLARSLGCSFQRIQFTPDLLPSDITGITFFNQKKGEFEFRPG
                     PLLAQVVLADEINRATPRTQSALLEAMEERQISVERETIRLPRPFIVVATQNPIELEG
                     TFPLPEAQIDRFLMRLRLDYPSQTEERLILQRFREEQPLDDLQPVLSASALQTLQAFI
                     RRVRVEPTLETYIVQLVQATRNHSGIELGVSPRGTLALYRASQAYAAIHGRAYIIPDD
                     VKKVARPVLAHRMIATSQARLHGRVMEQIVDDVLHSVAVPVEL"
     gene            3686..5047
                     /locus_tag="VGT44_06515"
     CDS             3686..5047
                     /locus_tag="VGT44_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEV2580489.1"
                     /translation="MNRKWYYTTLAIILFSLVIRQPLLLLLGILALLVLASIDIWANY
                     CMRNLRYQRELSARRVLFGEAFTFSITLENAKLLPLPWLEIEESISRSLVFADHNARV
                     RQSSASFVMESLFSLRWYERLTRRYTVTCAQRGVHTIGPTTLRSGDVFGFLNRQETIE
                     EREYLMVYPMVVPLTRFSLPARHPFGDYNAPRRLLEDPSRVIGVREYQYGDEMRRVHW
                     KATARAMQLQSKVYQPTTTYTMVIFLNLMQHLDVWYGIHPELQELAICAAASVANWAL
                     DEGLAVGLYANTMMYMPEFGMKLPKLPAPGSEPGDDEVSVESIVAEQLQRRRIHLPPA
                     SSEDQRRRIMETLARIQTYFGSPLEDMIQVERTRLPAGATVVVITAVISEPLLDILSR
                     VRSSGHAVTLIFVGDIPMSKQLAGITVYHIGGEDTWKQFMQDYTAPLEENSPRQLTGT
                     LNL"
     gene            5130..5999
                     /locus_tag="VGT44_06520"
     CDS             5130..5999
                     /locus_tag="VGT44_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580490.1"
                     /translation="MNQTKLDLHQNDIDLEISDLPGTDHNPASRAPAFIRALAEHPRL
                     RVRFWRLGTAALAFILLVLVLSSSFPQIQQGLQYLFAPPSPTATIQLPGLSSAQAIPL
                     HGRQKTSITIAVNSKPLIVPLTEALGPVPSACLHTTPTRAFDAPSFSPGLGALPMWVT
                     GFSNPAAGQRAVLDHLQRAQPPEAGWFQQVLLVSSTNFTGAITIQGGIEGSILPLWFG
                     VFPPGIAPRSSFGLIQTITVHPLDASFSNHTTGDQAWSILPINLYIAQAGCYYLKATW
                     NTGVWTAYFAAGK"
     gene            complement(6044..>6681)
                     /locus_tag="VGT44_06525"
     CDS             complement(6044..>6681)
                     /locus_tag="VGT44_06525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2580491.1"
                     /translation="PRRMFLFKIGRFDPDKRWIMAMEAVARLKYSGHPVTIFVRGGIE
                     PHGADVLRHAYNMGLHIKDVEARRPSLEQCTELIAKAGPADIYNLRFFLPEEFVRITY
                     RAADATLANSGHEPFGLVGLEVMAAEGLAVTGSTGEDYAISLENAIVTETEDPDEIVG
                     YLLYLRRHPDEQERIRQAGRRTSEQFLWDQVIENLVSKLEFLGRKQDIVFV"
BASE COUNT         1449 a         2175 c         1771 g         1286 t
ORIGIN      
        1 ccattttccg ggatgtaatc ctgatcgttg gtgttgtttt cgactcgcac ataaagcggg
       61 tcgagcaggt cattgaccat cttctccatg tagggaaaat cgagcgaacc cgcgtcgaat
      121 gtcaacgtcc cgaccaggtg gacctggatc agtggacgca gcacatcgcc ctcgaagttt
      181 ggacgttcaa cgcggcaacg cctgtcgagc acatcataaa gttcctgtgg ttcgcttgcg
      241 ccgccaaccg gaacttccag gcgcacgaac ggtcggcgtt tgctgacgag atgggtggcg
      301 cgatggcggc tgatccccac atcttcatcg ctttcttgct catccgtatc gatctcgaca
      361 tggtagtagc cgcgatcttc ccaggcggcc tcatcggctc cgacggtttc ggttgagccg
      421 ggattataaa tccagccgtc gaactcgaag ggtttatgca catggccgag cgccaggtaa
      481 tcgatagagt tgcgcagcgc ctggaactgc gacatagtcg gcagcccctg cacgcgcggc
      541 actacgccgt ccaccccggt atgcatcatc agcaggcgat actcgatgcc agccgcgtct
      601 tcatccgcgc gcgccgccgc cagcgccagc gccataccct ccatgctgcg cgtcgtcgtc
      661 gccccctgcc acggcaaacc atagacgcgc aggcggccac ccagcacatc tacatgcgaa
      721 ccttccatgc tctcctggtc ccaggccgtg atgaccggcg cgccattctg catacgggga
      781 ttcagcagaa ccaggtagcc ctggtgacac aggaattgca gccaggagat accctcgcga
      841 taataactgc gatcatggtt gccttcgatg gcgattaccg gaataccgcg atctttgagc
      901 cgctgcagcc cctcgatggc atgaatgagc gtcagcgcgt cgatggcgcg tttgtgaaac
      961 aggtctccgg cgatcaccat aaaatcaatc ttgcgattga ccgcctcggc agtgatatcc
     1021 cagaaggcgc ggctaaagtc gttgaagcgc tcgcgcaccc catattgctc gtagccgagg
     1081 tgtgtatcgg cgatatgtat aaacgaagct cgcataacat cccttccaat ccgacaatat
     1141 ctagctcggc ccggtcatca gcgcgcgcac tgccgccgcg accagcacca gcgagatgag
     1201 gctatagatc aggagatcgc gccggggctg cttgcccccg gtagcgtagg tcacggcgac
     1261 aggtatggcc agggccacga tgccgccata gacaaagtgc aggtagaaca ggctatcgcc
     1321 cggcggaccc ggtctctgcc ccagcagcag caggataatg ccgagcagcg cctgcaacag
     1381 gctcaccacc gccgtaaaaa taagcgagat gcgccagggg cgaatgatgg tctgcttctt
     1441 cataaaaaag aggatcaaac cccatacgcc cgtaatggtc cccgatgcca gcaccagaac
     1501 catgttgaaa aagtgcaaat tgctaacaat atcaaccata atgtttccta aaatggcgcg
     1561 catacgccgc atacgcgctc tatacactgt tcttcttcaa ggacagaata atggatattc
     1621 cgccagccct actatagcgc gtctcttcaa ttcaggcaag tagggagaag accagggcga
     1681 tgctggccaa cttgatattc ttacaacatt tttaccccga attacccatg cgattgaaca
     1741 ccagaaagcg agtttagtat aatagtagac ataacatggg caaacgacgc ttctggcaag
     1801 aaattagcgt ataataatat gtctacggta tggacatggc atggactagg aggaagaacg
     1861 cgatgcctaa tcatccagag atggaggagc gcaaccagcc cccgacatcg gggaaactgg
     1921 tgctgccctc gctgcccgac acacccggtc tacctggcac attccctacc ccgcgacctt
     1981 atactgaaac gtacaatgca caaatcaccc cggcgcagtt gattcgcccg aaaggaccgg
     2041 ctgctccaca tggtttgctc gcaagactcg cgttttactg gcgcaaggac ccggcttaca
     2101 aagtatttat gcttgcggtg gtcgcggtcc tgctggccag tattgtgttt gtctcgatgg
     2161 ccggggcagc atggctgggc aagcccttct tcggcagccc ctatacgcaa agcccgccga
     2221 cggtcacagc tcccaccggc acggtcgatc tccatcccac gtttgggccg cccgccgggg
     2281 gtacaggcag cgggcagtcg agccagccgc ccgcgcaaag cactcccgcc ctgggcaaca
     2341 ccccaacgcc ctcaggcagc ccttcgccac agccgtcccc gacgcagccg gggcagggcg
     2401 gcacactgac ggtacagatt acaggctatc ttccggttgt gccgaacaat agtcatgtcc
     2461 ccatcaccgt cacaaccaat gaggccaatg taaccgtcaa tctctacatg caaagcaacg
     2521 gaaatccgcc atttaaacga ttcggtccgg ggattaccga ctcctccggc atggtcacaa
     2581 ttgattggtt cgtctcttac ttcagcatcg taaaaaggac aattactgtg tccattacag
     2641 cgataggaac cgatcaaaat gggcagagca tccggtcggc tccggtgatg gttcgtgtct
     2701 atgtgtaagg agcgccataa ggatatgcca tgacgcaaac cgatgaaatc cgccaggtgc
     2761 aagacgccgc gcgcgtcgtt cgccagaata ccagcaaggt catcgtcggc aaggaagaag
     2821 tcatcgacct gctgatggtg gcgctgctgt gcgaaggaca cgtgctgttc gaagatgtgc
     2881 ctggcatcgg gaaaaccacc ctggcgaagt cgctggccag gtcgctgggc tgttcctttc
     2941 aacgcatcca atttacgccc gacctgctcc cctcggatat cactggtatc accttcttta
     3001 accagaaaaa gggcgagttc gaattccgcc ccggcccgct gctcgcccag gtcgtgctgg
     3061 cagacgagat caaccgcgcc acgcctcgca cccagtccgc cctgctggaa gccatggagg
     3121 aacgccagat cagtgtcgaa cgcgaaacca tccgcctgcc tcgcccattc atcgtcgtcg
     3181 ccacacagaa cccaatcgaa ctggaaggca cgttccctct gccagaagcg cagatcgacc
     3241 gtttcttgat gcgcctgcgc ctcgactacc ccagccagac cgaagaacgg ctgatcttgc
     3301 agcgtttccg cgaagagcag ccgctcgacg acttacagcc ggtgttgtcc gcgtcggctc
     3361 tgcagacgct gcaagccttc attcgccgcg tgcgcgtcga gccaaccctg gagacctaca
     3421 tcgtgcagct cgtgcaggcc acccgcaacc acagcggcat cgagctgggt gtcagcccgc
     3481 gcggcacgct cgcgctctac cgcgcatccc aggcgtatgc cgccattcat ggaagagcct
     3541 atatcatccc cgacgatgtc aagaaagtag cgcggcctgt cctcgcgcac cgcatgatcg
     3601 cgacgagcca ggcacggctg cacgggcgcg taatggagca aatcgtagat gatgtcctgc
     3661 acagtgtagc ggtgcccgtt gagctatgaa tcgaaaatgg tactacacca ctcttgccat
     3721 catcctgttt agcctggtta tccggcagcc attgctgctg ctgctcggca tcctggcgct
     3781 gctcgtgctg gcctccatcg acatctgggc caactactgc atgcgcaacc tgcgctacca
     3841 gcgcgagttg agcgcccggc gtgtgctgtt tggcgaagca ttcactttct cgattacgct
     3901 tgagaatgcc aagctactgc ctctgccctg gctggaaatc gaagaatcca tctcgcgctc
     3961 gctagtcttc gccgatcata atgcgcgtgt gcgccagagc agcgcgtcct tcgtcatgga
     4021 gagcctgttc agcctgcgct ggtacgagcg tttgacgcgc cgctataccg tcacctgcgc
     4081 ccagcgcggt gtgcatacca tcggcccgac cacgctgcgc agcggtgatg tctttggctt
     4141 tctgaaccgc caggagacga tcgaggagcg cgaatactta atggtctacc cgatggttgt
     4201 gccgctcacc cgtttcagcc tcccggctcg ccatccgttc ggcgactaca acgccccgcg
     4261 ccgcctgttg gaagaccctt cgcgcgtcat cggtgtgcgt gagtaccaat acggcgacga
     4321 gatgcggcgc gtccattgga aagctaccgc gcgcgccatg cagttgcaaa gcaaagtgta
     4381 tcagccaacc accacttata caatggtaat tttcctgaac ctgatgcagc acctcgatgt
     4441 ctggtacggc attcatcccg aactgcaaga actggccatt tgcgccgccg cctctgtcgc
     4501 caactgggcg ctcgatgaag ggctggcggt tggcctctac gccaatacca tgatgtacat
     4561 gcccgaattc ggcatgaaac tccccaaact ccccgctccc ggcagcgagc caggcgatga
     4621 tgaggtctcg gtagaatcca tcgtggccga gcaattgcag cgtcgccgca ttcacctgcc
     4681 gcccgccagc agcgaagacc agcgccgtcg catcatggag accctggcgc gcatccagac
     4741 ctacttcggc agcccgttgg aagacatgat ccaggtggaa cgcacacgcc tgcccgccgg
     4801 cgcaaccgtc gtcgtcatca ccgccgtcat cagcgaaccg ctcctcgaca tcttatcgcg
     4861 cgtccgtagt tcgggccacg ccgtgaccct catcttcgtc ggcgatatcc ccatgtcaaa
     4921 gcagctcgca ggcatcacgg tctatcacat cggcggcgaa gatacctgga agcaattcat
     4981 gcaagactac accgcgccac tcgaagaaaa cagcccgcgc caactaaccg gcacacttaa
     5041 cttgtaggaa tgcgattata gagacaaaca aataatacgg acataatgcg tagggacagt
     5101 ggggtgtgaa aatttgcgag gaagcataca tgaaccagac gaaactcgac ctgcatcaaa
     5161 acgacatcga cctggaaatc agcgacctgc ccggcacaga ccacaacccg gccagtcgcg
     5221 ctccagcttt catacgcgcc ctggccgaac acccccgact gcgcgtgcgc ttctggcgtt
     5281 tgggaacagc agcgctggcg ttcatcctgc tcgtcctcgt actctccagc agctttccac
     5341 aaatacagca gggcctgcag tatctcttcg cgccgccttc gccaacagca acgatacaac
     5401 taccggggct gtcctcagcg caggcaattc ctctgcatgg acgccagaaa acgtccataa
     5461 cgattgcggt caatagcaaa ccgctgatcg ttcctctcac tgaagcgctc ggccccgtgc
     5521 catcagcctg cctccatacg acacccaccc gggcattcga cgcgccctcg ttctctcccg
     5581 gcctcggcgc gctgcccatg tgggtcaccg gcttctccaa cccggcagcc gggcagcgcg
     5641 ccgtgctgga tcacctgcaa cgcgcgcaac cacccgaagc cggctggttc cagcaagtcc
     5701 tgctggtctc ctcgacaaac ttcaccgggg caatcaccat ccagggcggc atcgaaggca
     5761 gcatcctgcc actctggttc ggcgtcttcc cacccggcat cgccccacgc agcagcttcg
     5821 gactgatcca gaccatcacc gtacatcccc tggacgccag cttctccaat cacaccaccg
     5881 gcgaccaggc atggagcatc ctgcccatca atctctacat cgcccaggcc gggtgctact
     5941 acctcaaagc tacctggaac accggcgtct ggacagccta tttcgctgct gggaagtaat
     6001 ggaaacgaag cgccagggtc gccctggcgc ttcgccgctt gcatcacaca aacacaatat
     6061 cctgtttgcg tcccagaaac tccagtttac tgaccaggtt ctcgatcacc tggtcccaca
     6121 ggaattgttc cgatgtgcgc cgccccgcct ggcgaatgcg ctcctgctca tcaggatggc
     6181 ggcgcaggta aagcaggtag ccgacgatct cgtctgggtc ttcggtttcg gtcacaatcg
     6241 cgttctcaag cgagatggcg tagtcctcgc ccgtcgatcc ggtcacagcc aggccctcgg
     6301 cagccatgac ctccaaaccc accaggccaa acggctcgtg gccactgttc gccagcgtcg
     6361 catcggcggc gcgatacgtg atgcgcacga attcttcggg caggaagaag cgcaggttgt
     6421 agatatccgc cggacctgcc ttggcgatca gctcggtgca ttgctccagg ctgggccgcc
     6481 gcgcctccac gtccttgatg tgcagaccca tattgtaggc atgacgcagc acatccgcac
     6541 catgcggctc gatgccgcct cgcacgaaga tcgtcaccgg gtggccgcta tatttcagcc
     6601 gcgccaccgc ctccatcgcc atgatccagc gtttatccgg gtcaaaacgg ccaatcttga
     6661 agaggaacat gcggcgcgga t
//
LOCUS       DASZAK010000369         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_9274, whole genome shotgun sequence.
ACCESSION   DASZAK010000369 DASZAK010000000
VERSION     DASZAK010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9274"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..989)
                     /locus_tag="VGT44_06530"
     CDS             complement(168..989)
                     /locus_tag="VGT44_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEV2580492.1"
                     /translation="MIYVAGIDVGSTQAKGVILDEQGALVSKAIIDMETNMGTAAEEI
                     FQRVLERANLRASAIAFTVATGYGRYRVTFGDLQVTEISCHARGALAEFPGTRTVLDI
                     GGQDTKAIRIGDSGEVTDFAMNDKCAAGTGRFLGAASFALEMPLSELGPLALQSTRPV
                     RITTTCTVFAETEIIGHLAKGHLPEDVLMGMHYSIATRCASLVRRVGLVPEITFSGGV
                     SRNQAMVALLQQVLGTPLNVSTQAQFMGAIGAALYALDHVHAGRWSEHLLKEGAR"
     gene            complement(991..2289)
                     /locus_tag="VGT44_06535"
     CDS             complement(991..2289)
                     /locus_tag="VGT44_06535"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEV2580493.1"
                     /translation="MSAISTTTEGRLDARAAGQRLVNQWLGSLGTARARGQPVAYVFV
                     MGNAIEILRAFGFEMAFPEINSLQTGVRKAAPDFIAKAEDFGFSPDVCSYVKADVGLM
                     LSNMQHPSGVQMPVPDLAIASSLCNVYIKWAEIWERRFGTPAFVLDIPGKRYAAWKSP
                     PGSPQHLADARWVEGQLRDLIARCEQITGKTFESDRLAEVEAHVNEMVRLWIEILAFN
                     QHRPAPYNSMLDGLTYMGVLNAWRGTPEGVAYMRLLHAEIAGKVARGEGRVPHERFRL
                     VFSGTPCYVNMRRLIELFESHGGVFAASDYLTYAAGGLDSAGLFYDTSRPLESLAEVT
                     TLASQMGLSNHFFSHHTLAQQMKDFAADGIVYHGIKSCRTVATSMADNREYVTTRHGL
                     PTLYIESDLIDPRYWSDAQIKNRVDAFFEVLEQRAQLEGR"
     gene            complement(2306..>2914)
                     /locus_tag="VGT44_06540"
     CDS             complement(2306..>2914)
                     /locus_tag="VGT44_06540"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEV2580494.1"
                     /translation="LYCVRRDDPARLSTVETYLLVRAGTRMRREEHNVLLRAALRGLA
                     GRPVRPRDKPRVVFLGGFCEQPPLEMIETIEDACFIVDDDLLIGQRWITGDIPLDEGD
                     PVWALAESYIEHSASSPVQYDARKAKEEMLMRMLRDARAEAAIITAAKFCEPGLDDQL
                     ALSHRLDAGKVPYLVLEFEEKMTSFEQMAMQVETFAESLLFQ"
BASE COUNT          569 a          951 c          852 g          542 t
ORIGIN      
        1 atgcttcgtt acccccgcct cttcgagcag atcctcatag acccccgccg cagcgacagc
       61 cggtttatag ccgctgcgcc gcagcgcggt ggcacgcacc tggcgcgtct catccatcaa
      121 tacggctttg gtgtacgtcg aaccgatgtc gatcccaacg gcgagaatca tcgcgcaccc
      181 tccttgagca gatgctctga ccagcgaccg gcgtgtacat gatcaagcgc gtacagggcg
      241 gcgccgatag ctcccatgaa ctgcgcctgc gtgctgacgt tgagcggcgt accgagtacc
      301 tgttgcagca gcgcgaccat cgcctggttg cgcgaaacac caccgctaaa tgtaatctca
      361 gggaccagcc cgacccgccg cacgagcgag gcgcaacgcg tcgcgataga gtaatgcata
      421 cccatcagca catcttcggg caggtgacct tttgccaggt ggccgatgat ctccgtttcc
      481 gcgaacacgg tgcaggtggt cgtaattcgt accggcctcg ttgactgcaa ggccagcgga
      541 cccagttcag agagcggcat ttcgagcgca aatgacgcgg cccccaggaa gcgtccggtg
      601 ccggctgcgc atttgtcatt catggcgaag tcggtcacct cgccggaatc cccgatgcga
      661 atcgccttgg tatcctgccc cccgatatcc agcaccgtgc gcgtccccgg aaactctgct
      721 aacgccccgc gcgcgtggca gctgatctcc gtcacctgta ggtcaccgaa agttacacga
      781 tagcgaccat aaccagtggc aaccgtgaaa gcaatcgcgc tggcccgcag gttcgcacgc
      841 tccagcacac gctggaagat ttcctccgcc gctgtaccca tgttggtttc catatcaatg
      901 atggctttgc tgacgagcgc gccctgctca tccaggatca ctcctttggc ctgggttgag
      961 ccgacatcga tacctgctac atagatcatc tcatcggccc tccaactgcg ctcgttgctc
     1021 cagcacctcg aagaacgcat ccacgcggtt cttgatctgt gcgtccgacc agtagcgcgg
     1081 atcgatcaga tcggactcga tatagagcgt gggcagacca tgtcttgtcg tgacatactc
     1141 gcggttatca gccatgcttg tcgcgaccgt gcggcagctc ttgatgccgt ggtagacgat
     1201 gccatctgcc gcaaagtcct tcatctgctg cgccagggtg tggtgggaga agaagtgatt
     1261 cgagagcccc atctgcgagg caagggtcgt gacctcggcc aggctctcca gcggtcgaga
     1321 ggtatcgtag aagagacccg cgctatccag gccgcccgcc gcatacgtga ggtagtccga
     1381 agcggcaaag acaccgccgt gactctcaaa gagttcgatc aggcggcgca tattcacata
     1441 acagggggtg ccgctgaaaa caaggcggaa gcgctcatga ggaacgcgac cctccccgcg
     1501 cgcgaccttg cccgcaatct cagcatggag caggcgcatg tacgcgacgc cttcgggggt
     1561 gccgcgccag gcgttcagca ctcccatgta ggtgaggccg tcgagcatcg agttataggg
     1621 agccggtcga tgttgattaa aggcgagaat ctcgatccac aggcgcacca tctcgttcac
     1681 atgcgcctcg acctctgcca ggcggtcgct ctcgaatgtt ttgccggtga tctgctcgca
     1741 gcgcgcaatg aggtcgcgca actggccctc cacccagcgc gcatcggcca ggtgctgggg
     1801 actgccgggg ggcgacttcc acgcggcata acgcttgccg gggatgtcca gcacgaaggc
     1861 aggtgtgccg aagcgccgct cccagatctc ggcccacttg atgtagacgt tgcagaggct
     1921 tgaggcgatg gccagatcgg gtaccggcat ctgcacgccg gagggatgct gcatattgct
     1981 gagcatcaag ccgacatcgg ccttgacgta actgcacacg tccggcgaaa agccgaaatc
     2041 ctcggccttc gcgatgaagt ccggcgcggc cttacgcacg ccggtctgca aggaattgat
     2101 ttccgggaaa gccatctcga agccgaaggc gcgcaaaatc tcgatggcat tgcccatgac
     2161 gaagacatac gcgacgggtt ggccacgcgc gcgcgcagtt cccagggacc ccagccactg
     2221 gttaaccagg cgctggccgg cggcgcgcgc gtccagtctc ccctcggtcg tcgtgctgat
     2281 agcgctcaaa ccctgcctcc tttgctcact ggaagagcag cgattcggcg aaggtctcca
     2341 cctgcatcgc catctgctca aaactcgtca tcttctcttc aaactcaagt acgaggtacg
     2401 gcaccttccc ggcatcgagc cggtgcgaaa gcgcaagttg atcgtcgagg ccaggctcgc
     2461 agaacttggc ggcggtaatg atggccgcct cggcccgcgc atcccgcagc atgcgcatca
     2521 gcatctcttc cttcgctttg cgcgcgtcgt attgcaccgg gcttgacgct gaatgctcga
     2581 tgtaactctc cgccagcgcc cacaccgggt cgccttcatc tagagggatg tccccggtaa
     2641 tccaacgctg accgatcagc aggtcgtcat ccacaatgaa gcaggcatcc tcaatggtct
     2701 cgatcatctc gagcgggggc tgctcgcaga agcctcccag aaacacgacc ctgggcttat
     2761 cgcgcgggcg caccggtcgc ccggccagcc cgcgcagggc cgcgcgcagc agtacgttat
     2821 gctcctcgcg gcgcatgcgc gtcccggcgc gcacgagcag gtatgtctcc accgttgaga
     2881 ggcgcgccgg atcatcgcgc cttacgcagt acaa
//
LOCUS       DASZAK010000370         4303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_541559, whole genome shotgun sequence.
ACCESSION   DASZAK010000370 DASZAK010000000
VERSION     DASZAK010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541559"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3555
                     /gene="metH"
                     /locus_tag="VGT44_06545"
     CDS             <1..3555
                     /gene="metH"
                     /locus_tag="VGT44_06545"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEV2580495.1"
                     /translation="LAQRVLIFDGAMGTSIQAYQLTAQDYGGQATEGCNEYLILSKPS
                     VIEEIHTGFMEAGCDVLETDSFTGSRLKLDEYGLGAQTREINLAAARQARRIADRYST
                     LEQPRFVAGSVGPTGMLPSSDDPMLSNITYAQLVEIFREQGVALLEGGVDLFLIETSQ
                     DMLEVRAAITGLRRAMQQAGRAIPIQAQVSLDTSGRMLLGTDVAAVMTTLVGLRVDIV
                     GLNCSTGPEYMREPVRFLGENCPLPISTIPNAGMPLNVNGLAVYPMEPEPMAAALREF
                     ITEFGVNIVGGCCGSSHEHLRAIVRACRGVARRPRPIADARASLPFVSSGIRATAMRQ
                     DPAPLLVGERVNSQGSRKAKQALLADDYDSLLAIGREQVEGGAHVLDICVALTERIDE
                     AQQMARTVKKLSMGVEAPLVIDSTEPEVIKAALETYPGRAIINSINMENGRQRIESVL
                     PMALEHGSAVVALTIDEIGMGKTAARKVEIAHAIYDICVNEYGLSPDALIFDTLTFPV
                     TTGQEELQTAAIETLEAIRQVKAELPGVFTILGVSNVSFGLKPHERAVLNSVFLYHAV
                     RTGLDMAIVNPTHITPYAEIDAEQRQLADDLIYNRPDALPRYIAYFEAHEPQADEASA
                     KADPTEGMNTAERIHWQVLHRRKEGIEPLIEQLIAERAADLGIPSDEAAVNVLNTVLL
                     PAMKDVGDRFGAGELILPFVLQSAEVMKRAVARLELFLQKQEGYTKGKVVLATVFGDV
                     HDIGKNLVGTILSNNGYTVYDLGKQVPLNTILDKAIEVNADAIGLSALLVSTSKQMPL
                     CVQELHKRGLRFPVIVGGAAINRSYGRRILFVDQQTPESAPQPYDAGVFYARDAFEGL
                     EIIDKLTSAPEQRARFVEQVKAEALRERLKKSAVEPVPDQANASIPAGPSQNIRRDVS
                     IPTPPFWGPRVLERIGLEDVAACMDLNTLYRLHWGGKAHGAEFQRLVEQDYRPRLERM
                     LLEARQQRYLHPKAIYGYFPCQSSGNELIIYDAQAYQADRRTLNEVMRFSFPRQRQRD
                     RLCLADYFASRESGSVDVVAFQVVTMGQEASEAVQRLQQAGNYSEAYFVHGLAVEMAE
                     GLAEYTNRIIQRELWPGEQQTSANQRGRRYSWGFPAIPDLEDHSKVMQLLPVSRALGV
                     QLTSAFQFDPEQTTAAIVVHHPQATYFAAREEAALTRS"
     gene            complement(3674..3943)
                     /locus_tag="VGT44_06550"
     CDS             complement(3674..3943)
                     /locus_tag="VGT44_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580496.1"
                     /translation="MVSLTTSGTLEFAAEVDWLQWVAPEDDDPGKDQAQSGHVYGLAD
                     TDDDGEEDENTVAREDERLNIPPGPQDSGPPSEPPPDPKPTGKSY"
BASE COUNT          888 a         1450 c         1180 g          785 t
ORIGIN      
        1 ctcgctcagc gcgtcctcat cttcgatggc gcgatgggta ccagcatcca ggcgtaccag
       61 cttaccgcgc aggactacgg cgggcaggcc accgagggct gcaacgaata cctgatcctg
      121 agcaagccat cggtcattga ggaaatccat accggcttta tggaggcggg ctgtgacgtg
      181 ctggaaaccg attcgttcac cggcagccgt ctcaagctcg atgaatacgg gctgggcgcg
      241 caaacgcgcg agatcaacct ggccgccgcg cgccaggcgc gccgcatagc cgaccgctac
      301 agcacgcttg agcagccgcg cttcgtggcc ggctcggtcg gtccgaccgg catgttgcct
      361 tcgtccgacg accccatgtt gagcaacatc acctacgcgc aactggtcga aatcttccgc
      421 gagcagggcg tagcgctgct ggaaggcggg gttgacctct ttctgatcga gaccagccag
      481 gacatgctgg aggtgcgcgc cgccatcacc ggcctgcgcc gcgccatgca gcaggccggg
      541 cgcgccattc ctatccaggc gcaggtctcg ctcgacacca gtgggcgcat gttgctcggc
      601 acggatgtcg ccgccgtcat gaccacgctg gtcggtctgc gcgtcgatat cgtcgggctg
      661 aactgctcga ccgggccaga gtatatgcgc gaaccggtgc gcttcctcgg cgaaaattgc
      721 ccgctgccca tctcaaccat ccctaacgcc ggcatgccgc tcaacgtcaa cgggctggcc
      781 gtctacccga tggaaccgga gccgatggcg gcagccctgc gcgaattcat caccgaattc
      841 ggcgtcaaca tcgtcggcgg gtgctgcggc agcagccacg aacacctgcg cgccatcgtg
      901 cgcgcctgcc gtggcgttgc ccgccgaccg cgccccatcg ccgacgctcg cgcctcgctg
      961 cccttcgtct ccagcggcat tcgcgccacc gccatgcggc aggaccccgc gccgctactg
     1021 gtcggcgagc gcgtcaacag ccagggcagc cgcaaagcca agcaagccct gcttgccgac
     1081 gactacgact cgttgctcgc catcgggcgc gaacaggtcg aaggcggcgc gcacgtgctc
     1141 gacatctgcg tcgcgctgac cgagcgcatc gacgaagcgc agcagatggc cagaaccgtc
     1201 aaaaagctct cgatgggcgt cgaagccccc ctggtcatcg actcaaccga gccagaagtc
     1261 atcaaggctg ctctggaaac ctaccctggc cgcgctatca tcaactcgat caacatggag
     1321 aatggccgcc agcgcatcga aagcgtcctg cccatggcgc tagaacacgg ctcagccgtt
     1381 gtcgcgctca ccatcgacga gatcggcatg ggcaaaaccg ccgcgcgcaa agtcgaaatc
     1441 gcccatgcta tctacgacat ctgcgtcaac gaatacggcc tgtcgcccga cgcgctgatc
     1501 ttcgacaccc tgaccttccc cgtcaccacc ggccaggagg aactgcaaac ggcggccatc
     1561 gaaacgctgg aggccatacg gcaagtcaaa gccgaattgc ccggcgtctt caccatcctc
     1621 ggcgtcagca acgtttcttt cggcctcaag ccacacgagc gcgccgtcct caacagcgtc
     1681 ttcctctacc acgccgtcag gacgggcctc gatatggcga tcgtcaaccc gacgcacatc
     1741 acgccctacg ctgaaatcga cgccgaacag cgccagctcg ccgatgacct gatttacaac
     1801 cggcccgatg ccctgccgcg ctacatcgcc tattttgagg cgcacgaacc gcaggccgat
     1861 gaagccagcg cgaaggccga cccgaccgag ggcatgaaca cggcagaacg tatccactgg
     1921 caggtgctgc accgccgtaa agaaggtatc gagccattga tcgagcagct catcgccgaa
     1981 cgcgccgctg acctgggcat cccttccgac gaagcagccg tcaacgtcct caacaccgtc
     2041 ctgctgcccg ccatgaagga tgtcggcgac cgcttcggcg cgggcgagct gatcctgccc
     2101 ttcgtactgc aatccgccga ggtgatgaaa cgcgccgtcg cccgcctcga actctttttg
     2161 caaaagcaag aaggctatac gaaaggcaag gtcgtgctgg caaccgtctt cggcgacgtg
     2221 catgatatcg gcaaaaacct ggtcggcacc atcctcagca acaacggcta taccgtctac
     2281 gacctgggca agcaggtgcc gctcaacacg atcctcgata aggccattga ggtcaatgcc
     2341 gatgccatcg gcctctccgc cctgctcgtc agcacatcca agcagatgcc gctctgcgta
     2401 caggagttgc acaaacgcgg tctgcgcttc cccgtcatcg tcggcggcgc ggccatcaat
     2461 cgctcgtatg ggcggcgcat cctctttgtc gatcagcaga cgccagagtc cgcgccgcag
     2521 ccctacgacg cgggcgtctt ctatgcgcgt gacgccttcg agggcctgga gattatcgac
     2581 aaactgacca gcgctcccga acagcgcgcg cgcttcgtcg agcaggtcaa agcggaagcc
     2641 ctgcgcgaac gcctgaaaaa atccgccgtt gaacctgtcc ccgatcaagc caacgccagc
     2701 atacccgcag gcccatcgca gaatatccga cgcgatgtct ccattcctac gccgcccttc
     2761 tgggggccga gggtcttaga gcgcatcgga cttgaagatg tggccgcctg tatggacctc
     2821 aatacgctct accgactgca ctggggcggc aaggcgcacg gggccgagtt ccagcgcctg
     2881 gtagagcagg attatcgccc gcgcctggag cgcatgttgc tcgaagccag gcagcagcgc
     2941 tacctgcacc cgaaagctat ctacggctac tttccctgcc aatcgagcgg caacgaactc
     3001 atcatctacg acgcccaggc gtaccaggcc gaccgtcgta ctctcaacga agtaatgcgc
     3061 ttttccttcc ctcgccagcg ccagcgcgat cgcctctgcc tcgccgacta cttcgcctcg
     3121 cgcgaaagcg gcagtgttga tgtcgtggcc ttccaggtcg tcaccatggg ccaggaagcc
     3181 agcgaagccg tgcagcgcct ccagcaggcc ggcaactatt ccgaggcata ctttgtgcat
     3241 ggccttgccg tcgagatggc cgagggcctg gcggaataca ccaaccggat cattcaacgc
     3301 gaactatggc cgggcgagca gcaaacatct gccaaccagc gcgggcgtcg ctatagctgg
     3361 ggctttcccg ccatacccga cctggaagat cactcgaagg tcatgcaact gctgcccgtc
     3421 agccgcgccc tgggtgttca attgaccagc gccttccagt tcgatcccga acaaaccacc
     3481 gccgccatcg ttgttcacca cccgcaggca acctacttcg ccgcccgcga agaagccgcc
     3541 ctgacccgct cataaggatg aggacgcctg cgccgcgcct gacgttcttc tgagtgcgga
     3601 aagaacaccc ggttacggcg ggcgacgcaa gcgtcccccc acgcatctgc cccactcccg
     3661 cccctacagg gggttaatac gatttgccgg tcggcttggg atcgggtggc ggttcagatg
     3721 gaggtcctga gtcttgcggc ccagggggaa tgttcaggcg ttcatcttca cgcgcgacag
     3781 tattctcatc ttcttctcca tcgtcatcgg tgtcagccag cccgtaaaca tgcccgctct
     3841 gcgcctgatc cttgcccgga tcatcatcct caggagctac ccattgaagc caatcgactt
     3901 cagcggcaaa ctccagggtt cctgatgttg tcagcgaaac catgatgtta cctcctttct
     3961 ttgtgaattt ttcccacaca caatcagtga aaatatcaca agtcgtttag tttatattag
     4021 tttatacgat tagtaagcgt tatttattac atgagtaatc ctttataaag ttatattgga
     4081 gatttctaat attctcttct caataaagaa gacgcgaatt cttaaaaaat ctaaaaaatc
     4141 tcatgcgttg catgagattg tcagaatttg gggctgccag ggcgatgccc agcatcgccc
     4201 tgaccgggta cacagatgcc atcgccctgg ccgggtgttc atctttgcaa aaaaacgcac
     4261 ccctggcgca catcacattt cctaacggcg taaaactgtg cta
//
LOCUS       DASZAK010000371         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_258447, whole genome shotgun sequence.
ACCESSION   DASZAK010000371 DASZAK010000000
VERSION     DASZAK010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258447"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1246
                     /locus_tag="VGT44_06555"
     CDS             68..1246
                     /locus_tag="VGT44_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2580497.1"
                     /translation="MQKRIINFGIIGCGLMGREFGSAVARWCHLLDQDVQPRIVGVCD
                     ANPAMLDWFTGNFDSIQLITRDYHDLLASPAIDAVYCAVPHHLHERLYVDIIESGKHL
                     LGEKPFGIDLDANARIMQAVRAHPEVLVRCSSELPFYPGAQHILRFLREERFGTIIEV
                     QAGFLHSSDLDPRKPTNWKRRIATNGAYGCMGDLGMHVLHIPLRTGWYPRNVRALLSN
                     IVTERPGPDGAPEACETWDNALLACEVQTGGQHFPMLLDIKRIAPGETNTWYIRVTGT
                     RLSAEFSTKYPRTLRTMPYTPGAEQAWQAQDLGSQSAYPTITGGIFEFGFSDSIVQMW
                     AAFCDELAHPGQMRQPFACVTPQETMQHHRILTAALNSNSLAQVVEVGAPDGAPEDEP
                     "
     gene            1243..>2309
                     /locus_tag="VGT44_06560"
     CDS             1243..>2309
                     /locus_tag="VGT44_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HEV2580498.1"
                     /translation="MNAVEVIVAGHICLDVFPTLAGGTALFRPGRTVEAGPVLFSTGG
                     PVSNTGLALHKLGAATRLMGKVGDDLFGHAILDIIASSGPGLTDGMVIAPGEASSYSI
                     ILSPPNADRMFIHASGCNDTFGAEDVSYDLLEDARLFHFGYPPLMKRMYTNNGIELAA
                     MLWCVKALGLTTSLDLAQPDPLSEAGQADWRAILGSTLPHVDVFSPSIEEMLLMLRRP
                     LFDRIASGEQPIDHITPTLVSEIGQELLEMGAKIVALKLGVQGLYLRTTNVARLANVG
                     RCQVDDLIAWADRELWSPCFTAQVAGTTGAGDATIAGLLLGLLRGMSPYDTLTAACAV
                     GACSVEAVDATSAVRTWPETQA"
BASE COUNT          422 a          808 c          669 g          410 t
ORIGIN      
        1 atggcgctgc tccacgagga tgccacgccg gaacaggcca gcacgattct gcggaagggc
       61 tgagcgcatg caaaaacgta tcatcaattt cggcatcatt ggttgcggcc tgatggggcg
      121 cgagttcggc agcgccgtcg cgcgttggtg ccacctgctc gatcaagatg tccagccgcg
      181 catcgtgggc gtctgcgatg ctaaccccgc catgctcgac tggttcaccg ggaatttcga
      241 tagcatccag ctcatcacac gcgattacca cgacttgctc gcctcgcccg ccatcgatgc
      301 cgtctactgc gccgtgccgc accacctgca cgagcggctc tacgtggaca tcatcgagtc
      361 cggcaagcac ctgctcggcg aaaagccctt cggcatcgac ctggatgcca acgcgcgcat
      421 catgcaggcc gtgcgcgccc atcccgaagt gctggtgcgc tgctcatccg agcttccatt
      481 ctatcccggc gcacagcaca tcctgcgctt cctgcgcgag gagcgttttg gcacaatcat
      541 cgaggtacag gccggtttcc tgcacagcag cgacctcgac ccgcgcaagc ccaccaactg
      601 gaagcggcgc atagcgacca acggcgcgta tggctgcatg ggcgacctgg ggatgcacgt
      661 gctgcatatt cccctgcgca ccgggtggta cccacgcaac gtgcgcgccc tgctctccaa
      721 catcgtcact gagcgcccag ggccagatgg cgcgccggaa gcgtgcgaaa cctgggataa
      781 cgcgctgctg gcctgcgagg tgcaaaccgg cggccagcat ttccccatgc tgctcgacat
      841 caaacgcatc gcgccgggcg agaccaatac ctggtatatc cgtgtcaccg gcacgcgcct
      901 ctcggcggaa ttcagcacga aatatccccg gacactgcgc acgatgccct acacgcctgg
      961 cgcggagcag gcgtggcagg cgcaagacct cggctcgcaa tcggcatatc ccaccatcac
     1021 cggcggcatc ttcgagttcg gcttctccga ctcgattgtg cagatgtggg cagccttctg
     1081 cgacgaactg gctcatcccg gccagatgcg ccagcccttc gcctgcgtca cgccccagga
     1141 aaccatgcag caccaccgca tcctgaccgc cgcgctaaac tcgaacagcc tggcgcaggt
     1201 cgtagaggtc ggcgcgcctg atggcgcgcc ggaggatgag ccatgaacgc agtcgaagtt
     1261 atcgtagccg ggcatatctg cctggatgtc ttccccacac tcgcgggcgg cacggcgctc
     1321 ttccggccag gaagaacggt cgaggccggt ccggtgctgt tctccaccgg agggccagtc
     1381 tcgaacaccg gcctcgcgct gcacaagctc ggcgcggcca cgcgcttgat gggcaaggtc
     1441 ggcgacgacc tcttcggcca tgccatcctc gatatcatcg catcgtctgg gccaggactt
     1501 accgatggca tggttatcgc gccaggcgag gccagttctt attccatcat tctcagcccg
     1561 cccaacgctg atcgcatgtt tatccatgct tccggctgca acgacacctt cggcgcggaa
     1621 gatgtgtcct atgacctgct cgaagacgcg cgtctcttcc atttcggcta tccgccgctc
     1681 atgaagcgca tgtacaccaa taacggcatc gagctggcgg ccatgctctg gtgcgtcaaa
     1741 gcgttgggcc tgaccacctc gctcgatctc gcccagcccg acccgctcag cgaggcaggc
     1801 caggccgatt ggcgcgccat cctcggctcc acgcttcccc acgtcgatgt cttttcgccc
     1861 agcatcgagg agatgctgct catgctgcgc cgcccgctct tcgaccgcat cgcctccggt
     1921 gaacagccca tcgatcacat cacgcccacc ctcgtttcag aaatagggca ggaactcctg
     1981 gaaatgggcg cgaagatcgt cgcactcaaa ctcggcgtac agggtctcta cctgcgcacg
     2041 accaatgtcg ccaggctcgc gaatgtggga cgctgccagg tagatgacct catcgcctgg
     2101 gcggatcggg aactctggtc cccctgcttt accgcgcaag tcgcaggaac cacaggtgca
     2161 ggcgacgcga caattgctgg cctgctgctg ggactgctgc gcggcatgtc gccctacgac
     2221 acgctcaccg ccgcctgcgc cgtcggcgcg tgcagcgttg aagccgtcga cgccaccagc
     2281 gccgtgcgta cctggccaga gacccaggc
//
LOCUS       DASZAK010000372         1829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_648831, whole genome shotgun sequence.
ACCESSION   DASZAK010000372 DASZAK010000000
VERSION     DASZAK010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_648831"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            377..637
                     /locus_tag="VGT44_06565"
     CDS             377..637
                     /locus_tag="VGT44_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580499.1"
                     /translation="MRNRTARFIVGIVLVCVLALMGTGFVAAATHGGLGASGTARASV
                     SQDKPVFGATHVYMRDEAFSPARIQVVLGVCPWLEQKVGIGR"
     gene            complement(776..>1829)
                     /locus_tag="VGT44_06570"
     CDS             complement(776..>1829)
                     /locus_tag="VGT44_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580500.1"
                     /translation="LLVGIVIVWRLWGKENELLGLLTSFVLIVGGTIANGSGSLANFS
                     SAISFFLTIVGSLSFLLYWPATGMVLVTFPTGRFAPRWTWLVILLFIMQIFLYGPTYQ
                     VGPLLFAAERLLVWGSAFAVLAWRYRHLFTSVQQQQTKWLLYGYVPLYLVYLLYGALQ
                     SLPGLKSPDSLYLVVGPVFQQLFSLLVPLAFGIALLRYRLWDIDVLINRTLVYGLLTA
                     TLLVTYVLLVLGGQHLLAGVFGPNNAVVLVVSTLLVAALFQPLRQRVQQLVDRRFYRR
                     KYDAARVLAAFSATMRNEVDLEQVRERLLAVVQETMQPASAALWVRPPTSHSGPQAPW
                     RATSADPTRHEASAEW"
BASE COUNT          415 a          515 c          595 g          304 t
ORIGIN      
        1 agtatactcg tcgcttctct caaagagatc acagcggtgc tggtgaacat cacattgcta
       61 tcacaggatt ctggagcagg tcacgatgct atcacagaca cgcttcgagc gcacgagctc
      121 ccggcagagt tgtgagtggg ctgtgagatg acaagcgcct tttgtgacgc ctctgagaga
      181 gagatgtggt atatcctcaa cagggtaggt aggcgtaccc gcatcgggtg gctgctgttc
      241 caacgtcagc gaaacggcgg caggcagatg actgggtacg cgcggtcgca ataacgaagg
      301 gatgagtagc gtcgaggtgc ttgctggagc aggaaatcgc cgtagaacca tacctatcca
      361 gacgggaggg atcaagatgc gtaatcgaac agcgcggttc atcgtaggta tcgtcctggt
      421 gtgtgtcctg gccttgatgg gcacagggtt cgtcgcggct gcaacgcacg ggggcttggg
      481 agcgagcggc acggcgcggg cgtcggtctc gcaagacaag ccggtctttg gcgccacgca
      541 tgtctacatg cgcgatgagg cgttctctcc cgcgcgtatc caggtggtgc tcggggtttg
      601 tccatggttg gaacagaaag tcggaatagg aaggtagcct aaaagtgtaa atcccaagat
      661 tctgcggatg ctgatccggg gctggcctcg ctggtacccc gtggagagtg tgcgcattga
      721 tggtgctggc gtcgcgctcc tgggcggttc ctgtgttccc gcggaggcga agccatcacc
      781 attccgcact cgcttcatgc ctggtcggat cggcagaagt ggctctccat ggagcttgag
      841 ggccactgtg agacgttggc ggccgtaccc acagcgcggc cgatgctggc tgcatcgttt
      901 cctgcaccac ggccagcagc cgctcgcgca cctgctccag gtccacctcg ttgcgcatcg
      961 tggcactaaa ggccgccagg acccgggccg catcgtactt gcggcgataa aaccgtcggt
     1021 ccaccagctg ctgcacgcgc tggcgcaggg gctgaaacag ggcagcgacg agcagcgtag
     1081 agaccaccag caccaccgca ttgttcggtc caaacacgcc ggccagcaag tgttgcccgc
     1141 ccaagacgag cagcacatag gttacgagca gggtcgcggt gagcaggccg tagaccagcg
     1201 tgcgattgat cagcacatcg atatcccaca accggtaccg cagcagggcg atgccgaacg
     1261 ccagcgggac gagcagcgag aagagttgtt ggaaaacggg gccgaccacc agatagagcg
     1321 agtcaggcga ctttaggcca ggaagactct gaagcgcccc atacagcagg tagacgagat
     1381 agagaggcac atagccgtag agcagccact tggtctgctg ctgttggaca gaggtaaaca
     1441 ggtgccgata gcgccaggcg agcacggcaa aggcactgcc ccaaacgagg aggcgctctg
     1501 cggcgaacag caggggcccc acctgatacg tcggcccata caggaagatc tgcatgatga
     1561 agagcaggat cacgagccag gtccagcggg gagcgaagcg tccggtgggg aacgtcacca
     1621 ggaccatgcc cgtcgcaggc cagtagagca aaaagctgag agaccccacg atagtgagga
     1681 agaaagagat ggcggaagag aagttcgcca aggagccaga cccgttggcg atggttcccc
     1741 caacgatcag cacaaacgag gtgagcagcc ccagcagttc attctccttg ccccacagcc
     1801 gccacacgat cacgatccca acgaggagc
//
LOCUS       DASZAK010000373         1610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_613643, whole genome shotgun sequence.
ACCESSION   DASZAK010000373 DASZAK010000000
VERSION     DASZAK010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613643"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /locus_tag="VGT44_06575"
     CDS             complement(<1..1577)
                     /locus_tag="VGT44_06575"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580501.1"
                     /translation="MHEKSILTLEYPKIMEKVAREAAFSASKELVMALQPTPNLDEAR
                     HRLAYTTEAYNLIELRTDAGIQGARDIRKHLERAAREGILTPSDLLEVLATIQSAIHI
                     ARLTEKLDTESFPLLHQLGADIPQRPHLARRIEETISQEGEVLDTASPALRKLRSDIR
                     GANQRLQDRLRTLVHEFGNALQEPIITIRNDRYVVPVKAENRSQVRGIVHDQSASGAT
                     VFIEPMVVVELNNRLRQLQIEESQEIERILRQLSLEIGSEADALRRDVELLAEFDLHL
                     AKARYGRLMRCSEPRLNDAGYINLRNARHPLLTGKVVPINFHLGHEFFMVVITGPNTG
                     GKTVALKTVGLLTLMAQAGLHIPADEHSEIAVFEEVFADIGDEQSIEQSLSTFSSHMS
                     RIIEILQRIEQEIQRETPAIRGKLAETLVGTPESRRLRALVLLDELGAGTDPSEGSAL
                     ARAILTFLLERHVTTIATTHYSELKAFAHEQPGVVNASVEFDIETLSPTYRLNIGLPG
                     RSNALAIASRLGLDERI"
BASE COUNT          290 a          438 c          492 g          390 t
ORIGIN      
        1 ataatgcgct cgtcgagtcc caggcggctg gcaatcgcca gcgcattact gcgcccgggt
       61 aagcctatgt tcagccggta ggttggcgag agtgtctcaa tgtcgaactc gaccgaagcg
      121 ttgaccacgc caggctgttc gtgcgcgaag gctttcagtt cagaatagtg cgtggtggca
      181 atcgttgtga cgtggcgttc caatagaaat gtgagaatag cgcgcgccag ggccgaaccc
      241 tcgcttgggt ctgtacccgc gcctaactcg tcgagcaaca ccagcgctcg cagccggcgg
      301 ctctccggcg tgcctaccag cgtttccgcg agcttgccgc gtatagcggg tgtttcgcgc
      361 tgtatctctt gctcgatacg ctgcaatatt tcgataatgc gactcatgtg agaggagaag
      421 gtgctcaggc tctgctcgat gctctgttcg tcgccaatat cggcgaagac ctcctcgaag
      481 acggcgatct cagaatgttc gtctgccgga atatgcagcc cggcctgcgc catgagtgtg
      541 agcaggccaa cagtcttgag cgcaacagtt ttaccgcctg tgttggggcc tgtaatcacc
      601 accatgaaga attcgtgtcc caggtggaag ttgatcggta ctaccttgcc cgtgagtagt
      661 ggatggcggg cgttgcgcag gttaatgtat cccgcgtcgt tcaggcgcgg ctcgctgcag
      721 cgcatcaagc gaccatagcg agcctttgcc aggtgcaggt caaattctgc cagcaattca
      781 acgtcgcgcc tgagcgcatc cgcctcgctg ccaatctcaa gggagagctg gcgcaggata
      841 cgctcgatct cctggctttc ttcaatctgt agctggcgta gtctgttatt caactctact
      901 accaccattg gttcgatgaa gacggtcgcg ccactggcgg attgatcgtg cacgataccg
      961 cgtacctggc tgcgattctc ggccttgacg ggaacgacgt agcgatcgtt gcgtatagta
     1021 atgatgggct cttgtagtgc attgccaaat tcgtgtacca gcgtgcgcag gcgatcttgt
     1081 aaacgctggt tagccccacg gatgtcggag cgcagtttgc gcagcgccgg gcttgccgtg
     1141 tccagcactt caccctcctg gctgatggtc tcttcgatgc gtcgggccag gtgcgggcgc
     1201 tggggtatat cggcgcctaa ttgatggagc agtgggaagc tctccgtgtc gagcttttca
     1261 gtcaggcgtg ctatatggat agcgctctgg atggttgcca gcacctcgag caggtcgctg
     1321 ggagtcagaa tgccctcgcg cgcggcgcgt tcgaggtgct tgcggatatc gcgcgctccc
     1381 tgtatgcctg cgtcggtgcg aagttcgatc aggttgtagg cttcagttgt gtaggcgagg
     1441 cggtgccgcg cctcgtccag gtttggtgta ggctgtagag ccatcaccaa ttctttactg
     1501 gcggagaagg cagcttcgcg ggccaccttc tccataatct tgggatattc cagtgtgagt
     1561 atacttttct cgtgcatgcg tgtgtgaata agtcctttat ttaccaggct
//
LOCUS       DASZAK010000374         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_613644, whole genome shotgun sequence.
ACCESSION   DASZAK010000374 DASZAK010000000
VERSION     DASZAK010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613644"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..644)
                     /locus_tag="VGT44_06580"
     CDS             complement(168..644)
                     /locus_tag="VGT44_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580502.1"
                     /translation="MAITQNWQPASSTFYEDATIPLQNLQSAHRPRITEDSEWQAQHR
                     NALPQIPPRPTRSTRPLPQVSATPRPHPAQSEQATAAQSEKLLPGKYSLFRAYRSEAT
                     PATMKRLERRERIVFALLDGGRAIQDVARLTHQSDLAVARIIVRLYHQGYIEYIQG"
     gene            complement(899..>2189)
                     /locus_tag="VGT44_06585"
     CDS             complement(899..>2189)
                     /locus_tag="VGT44_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580503.1"
                     /translation="AGTRYVFMRGNSLWSELADGSEKQPDLLTASQVSVAPNWVVSNP
                     LPGRSAGDKLAYIDLITAKVHIIRSDGLQDITVNQPLLKAGITPASAWDTATGTAILQ
                     SLAWSSDGNNLAFLADPSGSGHTSIYIYSTLTHAVTAVPSPIQGSVSHASWSPDGARL
                     AFEVSQKSLTSIIDYNVHNLGILIIAENIGAGASAGDGVLAMDWSPNLPAITWSVGVI
                     GHVHSLWTRIIDGNTSANLLLSGDYAQAIYSRNGDYKTGSWLVVTSNAGLAGDIWRID
                     VIPGSRFVQLTTGRQVNYAQWSPDGSSIDYLDSLSVGVGTLHIVNATTRVDRLIAPNV
                     TNEPAPVWSANNQQIAFSTGSRVGIASVPVNTGIHYLALKGAASDLTWSLTSPHQLIV
                     SMNDGQQGIYLEDTLSNTAHLLDAQGTDGPLLWTQIP"
BASE COUNT          449 a          565 c          654 g          521 t
ORIGIN      
        1 ggaaatgctt gctcacggac atcccgcttt ccatcccagg tctacgtatt ataacgtata
       61 accaggtcaa ttgatatcaa ttttgcggcc atttttccta ttctcccaaa aagtcgtaaa
      121 cgcccggatg tggcttgaga atttggagat ttctcactga tttggggcta gccctggatg
      181 tattcgatat agccctggtg gtagagtcgc acaatgatgc gcgcaacggc cagatcgctt
      241 tgatgcgtca ggcgggcaac atcctggata gcgcgaccgc catccagtag ggcgaagacg
      301 atgcgctcgc gccgctccag gcgcttcatc gtggccggtg ttgcctcgga tcgataggcg
      361 cggaatagtg agtatttgcc gggtagcaac ttctcagatt gagcagcagt cgcctgctcg
      421 gattgggcag ggtgcgggcg aggggtagcc gacacctggg gcagtggcct ggtacttctg
      481 gttggacgag gcggaatttg gggtaaggcg tttctgtgct gcgcctgcca ttcgctgtcc
      541 tccgttatgc gcgggcgatg cgcactctgt aagttttgaa gaggaatggt ggcatcttca
      601 taaaatgtag aggatgccgg ttgccagttc tgagttattg ccacgtttac acctccataa
      661 cacacataca acccgatgct cccagatagc tatacaggtc tagacctgca ttatcaagta
      721 tactcttctt atgcgatttt tgcacatgag cagaccactt tttcgtgaag attccgtgaa
      781 gatattgtgt tttgctttat cattgtatat caacggtact ccgccccaat tgatctgatg
      841 aacttttcca actgtaacag gtaagggctg cgcccctaca gctaccttga ggaatgccct
      901 atggtatctg cgtccacagc agcgggccat cggttccttg cgcatcgagt agatgggcgg
      961 tattgctcag ggtgtcttcg aggtagatgc cctgctggcc gtcgttcatg gagacgatga
     1021 gctgatgagg cgaggtcagg gaccaggtca ggtcggaggc tgcgcccttg agcgcgagat
     1081 agtgaatgcc cgtatttacc ggcacactgg cgatgcccac acgcgagccg gtgctgaagg
     1141 cgatttgctg gttattcgca gaccataccg gggcgggttc attcgtgaca ttgggggcga
     1201 taagacgatc aaccctggtg gttgcgttca cgatgtgcag cgtgccgacg ccgacggaga
     1261 gcgaatcgag ataatcaatg gaggagccgt cgggcgacca ctgagcgtag ttgacttgcc
     1321 gaccggtggt gagctgtaca aagcgcgaac caggaatgac atcgatgcgc cagatatcgc
     1381 ccgccagtcc tgcattcgag gtgacgacca gccaacttcc tgttttgtag tcaccgttgc
     1441 ggctgtagat agcctgcgcg tagtcgccgg agagcagcag gttcgcgctt gtattgccat
     1501 cgatgatgcg cgtccacagg ctatgcacat ggccaatgac gccaacactc caggtgattg
     1561 ctggcagatt gggcgaccaa tccatcgcca ggacgccgtc gcccgcgctg gctcccgcgc
     1621 cgatattctc cgcaataatc aatatgccaa ggttatgcac attgtaatcg atgatgctgg
     1681 tgaggctttt ctgcgatacc tcaaaggcca ggcgcgctcc gtcgggcgac cagcttgcat
     1741 gactgacgct gccttgtatg ggtgatggca cagccgttac cgcatgtgtc agggtagagt
     1801 aaatgtatat gcttgtgtgg ccagagccgc tagggtcggc caggaatgcc aggttgttgc
     1861 catcgctgga ccatgccagg ctttgcagga ttgctgtgcc ggtcgcggta tcccatgccg
     1921 atgcgggggt gatgcctgcc ttcaagagcg gctggttcac ggtgatgtct tgcagaccgt
     1981 cgctacgaat aatgtgaact tttgcggtga tgaggtcgat ataggccagt ttgtctccgg
     2041 ccgatctgcc cggcagagga ttgctgacga cccagttcgg agcgacagag acctgggatg
     2101 ctgtcaggag atcgggctgc ttttcgctgc cgtcagcaag ctcactccac aagctgttgc
     2161 cacgcatgaa gacgtaacgc gtgccagcc
//
LOCUS       DASZAK010000375         2931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_43951, whole genome shotgun sequence.
ACCESSION   DASZAK010000375 DASZAK010000000
VERSION     DASZAK010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43951"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1356)
                     /locus_tag="VGT44_06590"
     CDS             complement(19..1356)
                     /locus_tag="VGT44_06590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580504.1"
                     /translation="MFASSVKRMKFLRALKSRPFAMIWIGQTISNLGDGIFYLALAWQ
                     VLLMTHSGTAMAIVLIAGIIPRLIFVLFGGVAADRLPRRLIILWSDGGRGVIVLLISI
                     LGFAGLLQFWHLLVQSFIFGVVDGFFNPAILAITPDLVEKEDLASANALVSLSGNVAQ
                     LVGPTVGAGFIALTGPMGAFAANALSFFISMAFLLSVRIPERHVTKPLEIQASAVEGS
                     EIEGVQALEGLPEESPAGRKGMRGVLADVQEGLVYVRNSRWVWVTIFSAALGNVGIMA
                     SLVTSMPKLVHDVYGQGAWLLGLISTTGAIGSILALILIGQATRLKKRGLLAYLSITV
                     SSIGLLIFGLPFPHAAAPFIAPLASILVDFGIAFFNTIYFTILQEMIPGDKLGRVISL
                     DTLGSFAMIPVGEAVGGILTDRIGPATVFIIFGLFNIVNSLIPLLVREVREME"
     gene            1760..>2931
                     /locus_tag="VGT44_06595"
     CDS             1760..>2931
                     /locus_tag="VGT44_06595"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2580505.1"
                     /translation="MNYAILGGGALGLGAAYRLARAGHEVMVFEQEELAGGLASGFRV
                     GETWLEKFYHHLFKTDTTVIRLIEELGLGDRLEWSHPRTVCLINGRCQQLDSPMTLLR
                     FSPLRIDERLRVGAVGALLKLSSSERYEGKTAEPWLRRWMGKHAYGMVFEPLFAAKFG
                     ALHDQIALPWFWARFHDRTTYLGYLRGGFQLLYEQLVERIAQAGGKVLLGTRVEGAQT
                     LDDGRWEVRTSKGTWTFDRVISTLPTRLTCRLIPALPQDYRARYDWGQAYGAQCLILA
                     LDRQVTDSYWINPCDAGYAFTSLVEHTNYRPPAEYGGRRLLYLGNYRPMDDPLFTMSK
                     DDIIQAFLPDLKRINPDFSADWIQESWLFSAPFAQPIVTTEYRQHIPPLRAPLPNL"
BASE COUNT          662 a          878 c          850 g          541 t
ORIGIN      
        1 agatctatgc caaaaaatct actccatctc gcgcacctcg cgcacgagca gcgggatcaa
       61 gctgttcacg atattgaaca ggccgaatat gatgaagact gtcgccgggc cgatacgatc
      121 ggtcaggatg ccgcccaccg cctcgccgac ggggatcatg gcgaaggagc caagtgtatc
      181 cagactgatg acgcgcccca gtttatcgcc ggggatcatt tcttgcagga tggtgaagta
      241 gatcgtgttg aaaaaggcaa tgccgaaatc caccagtatg ctggccagcg gcgcgatgaa
      301 cggcgccgct gcatgcggga acggcaggcc gaaaatcagc aggccgatgc tgctcacggt
      361 tatcgagaga taggccagca gaccgcgttt cttcaagcgc gtggcctgtc ctatcaagat
      421 caaggccagt atggaaccaa tagccccggt tgtgctgatc aggccaagca gccacgcgcc
      481 ctggccgtag acatcatgca ccagcttggg catggaagtg accagcgatg ccatgatgcc
      541 gacgttccca agagccgcgc tgaagatagt gacccacacc cagcgcgaat tacgcacata
      601 caccaggccc tcctgaacat ccgccagcac acctctcatg ccctttcgcc cggctggcga
      661 ctcttccggt agaccctcaa gggcctgaac gccttcaatt tcgcttccct caacggcaga
      721 tgcctgtatc tcaagaggtt tggtcacgtg ccgctccggg atgcggacgg agaggagaaa
      781 ggccatcgag ataaagaagc tcagggcgtt ggcggcaaaa gctcccatcg ggccggtaag
      841 ggcgataaag ccggctccca ccgtaggtcc caccagttgc gccacgttgc cgctgagcga
      901 aacgagcgcg ttggccgagg ccaggtcctc tttctcgacg agatcgggcg taatcgccag
      961 aatggcagga ttgaaaaagc catcgacgac gccaaagatg aacgattgga caagcaggtg
     1021 ccagaattgc aggagacccg caaagcccag aatgctgatg agcaggacga tcacgcctcg
     1081 accgccgtcg gaccagagga taatcaggcg gcgcggcagg cggtctgccg ccactccccc
     1141 gaacaggaca aagatcaggc gaggaatgat gcccgcaatc aagacaatcg ccatggcagt
     1201 gccggagtgg gtcatcagga gaacctgcca ggccagggcg aggtagaaaa tgccgtcgcc
     1261 cagatttgaa atggtctgac caatccagat catggcaaat ggacgtgatt ttaaggcgcg
     1321 aagaaacttc atgcgtttca ccgaagacgc gaacatgagc gtaaacattc cttttctctg
     1381 ctcgtcccta cagacccctc tatcctcgcc ggacacagca aagcgcaaac gaagagagaa
     1441 gaaacttgtc aacaggtcga cggccagtcg gaaatgagcg caaaatggcc ctcaggccat
     1501 caattacata tcaaaaggat cacgatggga gaagaccgta gccatccggc gcgatgctac
     1561 gggcgcagag agcatgttag cgtaacttca taggagataa tcctttgctg attatcgttt
     1621 gtcttcacgg gtatagtata cacgaaaaag cgctgcggta aaagaccttt ttcataaaac
     1681 ctacctcatt tccttcatgt atgttacaat actcccgtct tatcacagcg atgaacatac
     1741 ttataaggaa cctacataca tgaactacgc tattcttggc gggggagcgc tggggttggg
     1801 agcggcctat cgcctggcgc gggcgggcca cgaggtgatg gtttttgagc aggaagagtt
     1861 ggcgggcggg ctggcctccg gctttcgcgt cggggagacc tggctggaga aattctacca
     1921 ccacctgttc aagaccgata cgaccgtcat tcgtttgatc gaagagcttg ggctgggaga
     1981 tcggctggaa tggtcgcatc cccgcacggt ctgcctgata aatggcaggt gccagcagtt
     2041 ggactcgccc atgacgctgc tgcgcttctc gcccctgcgc atcgacgagc gcctgcgcgt
     2101 gggtgccgtc ggcgcgctgc tcaagctgag cagctcggag cgctacgagg ggaagaccgc
     2161 cgaaccctgg ctgcggcgct ggatgggcaa acacgcttat ggcatggtct tcgagccgct
     2221 cttcgccgcc aagttcggcg cgctgcacga ccagatcgcg ctgccctggt tctgggcgcg
     2281 tttccacgac cgcaccacct atcttggcta cctgcgcggc ggtttccagc tcctgtatga
     2341 gcaactggtt gagcgcatcg cccaggctgg cggcaaagtc ttgctgggaa cgcgcgtcga
     2401 gggcgcgcag acgctcgatg atggccgctg ggaggtgcgc accagcaagg gtacgtggac
     2461 gttcgaccgc gtgatctcca cgctgccgac gcgcctgacg tgccgcctca ttcccgcgct
     2521 gccacaggat taccgcgcgc gctacgattg ggggcaggcg tatggcgcgc aatgcctgat
     2581 tctcgcgctg gatcgccagg tcacggatag ctactggatc aacccctgcg atgccggcta
     2641 cgccttcacc agccttgtcg aacataccaa ctatcgccca cccgccgaat acggcgggcg
     2701 acgcctgctc tacctgggca actaccggcc tatggacgac ccgcttttta cgatgagcaa
     2761 ggacgacatc atccaggcgt tcctgccaga cctcaagcgc atcaatcccg acttcagcgc
     2821 ggactggatt caggaaagct ggctcttctc cgcgcccttc gctcagccga ttgtgacgac
     2881 cgagtatcgc cagcatatcc cgcccctacg cgcaccgctg cccaacctgt g
//
LOCUS       DASZAK010000376         6566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_185841, whole genome shotgun sequence.
ACCESSION   DASZAK010000376 DASZAK010000000
VERSION     DASZAK010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185841"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="VGT44_06600"
     CDS             complement(<1..1022)
                     /locus_tag="VGT44_06600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2580506.1"
                     /translation="MKQLKQHLPLLVVFFLALGVNLVYNLVVANGYVASSDAAWYNQI
                     AYTVAYHGCYCQYPGVQSISRPPLWPLILAIFYHLAPPVSFQQADASIAVFYSRLFYS
                     VLGSFTCVLVYLLARDLFGKRIGLITGCVAAFYPGIFIYDGWVYTESLYTFLVTAFTY
                     SLYRLQRTGKLRWAAWSGVALGLANLARPNGPVLLGMLIIWAVGLILAKGVPWQTILK
                     GVLLSGLVAAAIIAPWTYRNFQVSGSYVMVSLGGGDVLLGAYNNNVASGSTGLWTSPR
                     YVKPRPDVPPIVLAGHDAKGYTAADDKLATNAALQWIQSHLNEVPLLMFNRLKTMWTP
                     FTAENA"
     gene            complement(1026..2786)
                     /locus_tag="VGT44_06605"
     CDS             complement(1026..2786)
                     /locus_tag="VGT44_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580507.1"
                     /translation="MLLRGKQITPRAPGRAAIVIIALALALRIGLTLAGWPPTNSDES
                     TMGIMAIHIIHGKDFPIFLYGQLYMGALEAYIAAGLMHVGETLFALRLANMLLFTLAL
                     TSFYLLASLLYTKKVALITIALLSIGSVMMIFTELMAHGGYPEIISLGVTSFLLAAYL
                     ALTSNQQTPLYRQWKRLLAYFGWGLASALGFWGDYIMLPIILMTGLLILLFCWRELLK
                     GAILPVLLGLLIGGIPLILYNLSSPPTTSTLAVIWSLRNNFTPELTHSVYYQFRYFYE
                     AKGTLLVSLPMATGAPPLCFDSGWVVYGRGGGFAAFQCLNPQAPTGLIALSLIWSAGF
                     LVLWLLSTTHELAALWRLRRFAAKSPALRQEVIRHFARLALLLCAALILLEFTLSPVS
                     AVFSAAARYLTGLLIATPVIIAPLCGYARDRSVLQSLVANNPDSSPPRRSLFVPFSAV
                     LRWGGLLFIAVVLLAGTFSIFSELPTVRAVDGQQQALITDLTSIHVTHFYTDYWTCNR
                     LAFLSDERLICAVLDSNLNETHTRPPGYYAIVKGDPRSAYMFPLGSVEADKVARLFAQ
                     SKQPYRRSIFEGYVVYQPLK"
     gene            3011..4132
                     /locus_tag="VGT44_06610"
     CDS             3011..4132
                     /locus_tag="VGT44_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEV2580508.1"
                     /translation="MATTDGLEDIVAAHSQICDLDGKRGRLSYYGLDVHDLARYSTFE
                     ETAYLLWHGVLPTRAQLADVTAQLQAGRAIPAQVGELMRLFPLTATPMDVLRTAISAL
                     SSFDPDAGDNSVEANRRRSIRLTAVIPTITAAWDRLRNGKEPVEPLADGSHAANFLYM
                     LKGEQPDEATAKMLDVVLILHADHELNASTFAARVTAATLASMYAAITSAIGALSGPL
                     HGGANEQVMRMLLRIGEVDRAETYIQGALERKERIMGFGHRVYRTEDPRATHLRQMSK
                     ELGERTGDTRWYEMSHIIEEYIKSHKGLYANVDFYSASVYYSMGIPIDLDTPIFACSR
                     MSGWTAHVLEQFANNRLIRPRAEYVGPKDVQYTPIDERS"
     gene            complement(4178..6088)
                     /locus_tag="VGT44_06615"
     CDS             complement(4178..6088)
                     /locus_tag="VGT44_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2580509.1"
                     /translation="MARCLNPRANGGHENADSAKVCRSCDFLVQGARIGIYEVVSFIG
                     TGSYGQVYQVREPAPLSRLLALKVLRIDQYNEKARESFFSEARRIATMQHPNILPVYN
                     FGLLEDERPYLVMEYAPRTILDLFRNKDGTRRLAYAEELVPYLKQAADALFNVHENGL
                     IHQDVKPGNLLIGRSGQILLSDFGATFYLGMQTHASLGEVTGTAPYMPPEQWQGYPRR
                     DSDQYALAICAYELLAGRPPFVYARIEQMWNAHINEQPPAPQQWNPRIPVEVSAVLNR
                     ALAKDYHQRYRSVFEFAENYAAAAALAQARYVCQVCGHQNRSGAQRCSMCGSNADNRR
                     CPYCDTPVRFGQRCCSVCGRLTIPPALIAHSPLSGVTARQGRYIIQYVLRQNDATRSM
                     TAIALDTAANERKVTLKRWECADSPLERRRKEILYYERATERLAAFHHPLVPTVLDRF
                     AEGKHYYLVEQYIDGESIEEMLQKQLKPLPEREVIGYIYGILNVLLALEQQRPPMRHF
                     DISPANIIIEHARGRALLTGFPLPLPPLDLKPGSPNSSTTRKIALSAYMPVKDMPPDH
                     RTCIYMLAATAHHALTDVAPPHYPTFPPIRELNPRISPRLEAILSRALMEDRAMRYQS
                     YAEMKKDIQKIL"
     gene            complement(6229..>6566)
                     /locus_tag="VGT44_06620"
     CDS             complement(6229..>6566)
                     /locus_tag="VGT44_06620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2580510.1"
                     /translation="DEDARQLEAWERDATLAPPGGESLAATQQRICDAVEQLSQAHPD
                     QAVALVSHVGPIKALLCAALGAPLSTPSHIFLDPATISVIDWRDREHATVRLVNSHAH
                     LGWSSARWM"
BASE COUNT         1391 a         1809 c         2086 g         1280 t
ORIGIN      
        1 atggcgtttt ccgcggtgaa aggcgtccac attgttttga ggcgattgaa catcaacaaa
       61 ggaacctcgt tcaagtggct ctgtatccac tgcaacgcgg cgttggtagc aagtttatcg
      121 tctgcggcgg tgtagccctt cgcgtcatgg cctgccagca caatcggcgg cacatcggga
      181 cgcggcttga catagcgcgg cgatgtccac aagcccgtgg agccgcttgc cacgttgttg
      241 ttataggcgc ctaagaggac atcgccgccg ccgagcgaaa ccattacata cgagcctgag
      301 acctggaagt tgcggtaagt ccagggcgcg atgatggcgg ctgccaccaa tcctgatagc
      361 agaacgcctt tgaggatcgt ttgccagggg acacccttcg ccaggatgag gccgacagcc
      421 cagataatga gcattcctaa gagtaccggg ccgttggggc gcgccaggtt tgccaggccg
      481 agcgcgacgc cgctccatgc ggcccagcgc agcttgccgg tgcgctgcag gcggtagagt
      541 gagtaggtga aggctgtcac caggaaggta tagagcgatt cggtatagac ccagccgtcg
      601 tagatgaaga tgccgggata aaaggcggca acacatcccg taatcagccc gattcgtttg
      661 ccgaacaggt cgcgcgccag cagatagacc agcacacagg tgaacgagcc gaggacgcta
      721 tagaacaggc ggctatagaa gaccgcgatg cttgcatcgg cctgctgaaa actcaccggc
      781 ggcgcaaggt ggtagaaaat agcgaggatc agcggccaga gcggcggcct gctgatcgat
      841 tgcacaccgg ggtactggca gtagcagcca tgataggcga cggtataggc gatctggttg
      901 taccaggcgg cgtcagacga ggcgacatat ccattcgcca cgaccaggtt atagaccagg
      961 ttgacaccca gcgccaaaaa gaagacgacc agtaatggca gatgctgttt gagttgtttc
     1021 atagcttatt tcaacggttg atagacgaca tagccctcaa agatagaacg ccggtacggt
     1081 tgctttgatt gcgcgaagag gcgagcaact ttgtcggcct cgacggagcc gagcgggaac
     1141 atgtaggcgg agcgcgggtc gcctttgacg atggcgtagt atcccggcgg gcgagtgtgg
     1201 gtctcgttca ggttgctgtc cagcaccgcg cagattaagc gctcgtcgct caggaaggcc
     1261 aggcggttgc acgtccaata atcggtataa aagtgggtca cgtgaatgct ggtgagatcg
     1321 gtgatgagcg cctgctgctg gccgtctaca gcccgcacgg taggaagttc ggagaagatg
     1381 ctgaacgttc ccgccagcag gacaacggcg atgaagagca gaccgcccca gcgcagcacc
     1441 gcgctgaacg ggacgaagag actgcgccgg ggcggtgaag agtcaggatt gttagccaca
     1501 agcgattgta acacggacct gtcgcgcgcg tagccgcaga gtggcgcgat gatgacgggt
     1561 gtagcgatca gcagaccggt caggtagcgc gcggcagccg aaaagacggc ggaaacggga
     1621 ctcagggtga attccagcaa gatcaaggcg gcgcagagca atagcgcgag acgcgcgaaa
     1681 tggcgtataa cttcttgccg cagagcgggc gattttgccg cgaagcgcct gagtcgccag
     1741 agcgccgcca gttcatgcgt cgtcgagagc agccagagaa ccaggaagcc ggcgctccat
     1801 atgagcgaaa gggcgatcag tcccgtcggc gcttgcgggt tcaggcattg gaaggccgcg
     1861 aacccgccgc cgcgcccata gaccacccaa cccgagtcga agcagagcgg cggcgcgccg
     1921 gttgccatcg gcagactgac gagcagtgtg cctttagcct cgtagaagta gcgaaactgg
     1981 taatagacgc tgtgggtcag ttcaggggta aagttgttgc gcagactcca tataacggcc
     2041 agcgtcgaag tggtgggcgg agacgacaga ttgtagagaa tcaagggaat gccgccgatc
     2101 agcagaccca gcagaacggg caggatagct cctttcagca attcgcgcca gcaaaagagc
     2161 aggatcagca agccggtcat caagatgata gggagcatga tgtaatcgcc ccagaagccg
     2221 agcgcggagg ccaatcccca gccgaaatag gccagcaggc gcttccactg cctgtacagg
     2281 ggcgtctgct ggttggaggt gagggcgaga taggcagcga gcagaaacga tgtaacgccg
     2341 aggctgataa tttcggggta gcctccatgc gccatcagtt cggtgaagat catcatgacc
     2401 gagccaatgc tcagcagcgc gatggtgatg agcgcgacct tctttgtgta gagcaggctc
     2461 gccagcaaat aaaagctggt gagcgccagg gtgaacagga gcatgttggc gaggcgcagc
     2521 gcgaagagcg tctcgccaac atgcatcaac ccggcggcga tatatgcttc gagcgcgccc
     2581 atgtacagtt gcccgtacaa gaaaatgggg aagtccttgc catgtatgat gtggatcgcc
     2641 atgatcccca tcgtggactc gtcgctattc gtcggcggcc agcccgccag cgtcagaccg
     2701 atgcgcaagg ccagcgccag cgcaataatg acgatagccg ctctccctgg cgcgcgcggg
     2761 gtgatctgct tccctctaag cagcatgaac agatgcctcc atagatgata catgttttgc
     2821 cacaagatta taacgaaaca atgcggcgaa aagcgtcaaa gtagcggggt aagtacggtc
     2881 gcccttttct tttgagcagt atttacattg atttgtgttg acattgtttt ttgctgatga
     2941 ctataatacc tttacagaac cgcatattgt ttgttgtagt gaaaggttct ctgatcgaag
     3001 gagatcttct atggctacca ccgatggact cgaagatatc gtagcagcgc actcccagat
     3061 ttgcgacctg gatgggaaac ggggtcggct ctcgtactat ggtctggatg ttcacgacct
     3121 ggcgcgttac tcgacgttcg aggagacagc atatttgctc tggcatggtg tgctgccgac
     3181 cagggcgcag ctcgcggatg tcactgctca gctccaggct ggtcgcgcca tcccggccca
     3241 ggttggggaa ctgatgcgcc tgttcccgct gaccgcgacg ccgatggacg tgctgcgcac
     3301 cgccatttca gccctctcgt ccttcgaccc ggacgcgggc gacaactctg ttgaggcaaa
     3361 ccgccgccgc tcgattcgtc tgaccgccgt catcccgacc atcaccgccg cgtgggatcg
     3421 gctgcgcaac ggcaaagagc cggttgagcc gctcgcggac ggcagccacg ccgccaactt
     3481 cctgtatatg ctgaaaggcg aacagcctga cgaggcgacg gcgaaaatgc tcgatgtcgt
     3541 gctgatcctt catgccgacc atgagctgaa cgcctcgacc tttgccgcgc gtgtcacggc
     3601 tgcgacgctg gcgagtatgt acgcagccat cacctctgcg attggtgcgc tctccggccc
     3661 gctgcacggc ggtgcgaacg agcaagtcat gcgtatgctc ttgaggatcg gcgaggtaga
     3721 cagggccgaa acgtacatcc agggcgctct ggaacgcaag gagcgcatca tgggctttgg
     3781 gcatcgcgtc tatcgcaccg aagacccacg cgcgacccac ctgcgccaga tgtcgaagga
     3841 actgggcgaa cgcaccggcg atacgcgctg gtacgagatg tcgcacatca ttgaagaata
     3901 catcaagtct cacaagggac tgtacgccaa tgtggacttc tactcggcct ccgtctacta
     3961 ttcgatgggc attcccatcg acctggatac gcccattttc gcgtgcagcc gcatgtccgg
     4021 ctggaccgcc cacgttctcg aacagttcgc caacaatcgt ttgatccgtc cgcgcgccga
     4081 atatgtcggc ccgaaagatg tacagtacac gccaatcgac gagagatcat agctacaaaa
     4141 aatcttataa cacatcccac tctgcgagtg ggatgtgtta taagattttt tgtatatcct
     4201 ttttcatttc ggcgtagctc tggtagcgca tggcacggtc ttccatcagg gcgcggctca
     4261 ggatggcttc tagccgcggg gagatgcgcg ggttcagctc gcgaatggga ggaaaggttg
     4321 gatagtgcgg cggggcgacg tccgtcaggg cgtgatgcgc ggtggccgcg agcatgtaga
     4381 tgcaggtgcg atggtcgggg ggcatatcct tgaccggcat ataggctgaa agcgcgattt
     4441 tgcgcgttgt ggaggaattt gggctgcccg gtttcaggtc aagcggtggc aacggtaatg
     4501 ggaagccggt cagcagggcg cgcccgcgcg catgttcgat gatgatgttg gcgggcgaaa
     4561 tgtcgaaatg gcgcatcggc ggacgctgct gttccagcgc cagcaaaacg ttcaggatgc
     4621 catagatata gccgatcact tcacgctcag gaagcggctt gagctgcttc tggagcattt
     4681 cctcgatact ttccccgtcg atgtactgtt ccacgaggta atagtgtttc ccctcggcga
     4741 agcgatccag gacggttgga accaggggat gatggaaagc tgccaggcgt tcggtggctc
     4801 gttcgtagta gaggatctct ttgcggcggc gttcgagggg gctatcggcg cattcccagc
     4861 gtttcaaggt cactttgcgt tcgttcgcgg cggtgtcgag cgcgatggcg gtcattgacc
     4921 tggtggcgtc gttttgccgc agcacatact ggatgatata gcgcccctgc cgcgctgtga
     4981 cgcccgaaag aggactatgg gcgatcagcg cgggtgggat ggtcagccgc ccgcagacgg
     5041 agcagcagcg ctggccgaaa cgcaccggcg tatcgcagta ggggcagcgg cgattatcgg
     5101 cgttcgagcc gcacatggag cagcgctggg ccccactgcg gttctggtgg ccgcagacct
     5161 ggcagacgta gcgcgcctgg gccagcgctg cggcggcggc atagttttcg gcaaattcaa
     5221 agacgctgcg gtaacgctgg tgataatcct tcgccagcgc gcgattgagt accgcgctca
     5281 cctcaaccgg gatgcgtggg ttccactgct gcggcgcggg tggctgctcg ttgatgtgcg
     5341 cgttccacat ttgctcgatg cgcgcgtaga caaagggagg gcggcctgcc agcagttcgt
     5401 aggcgcatat ggccagggcg tactggtcgg aatcgcgccg gggatagccc tgccactgct
     5461 ccggcggcat gtagggggcg gtacccgtca cttcgcccag cgaggcgtgg gtttgcatac
     5521 cgagatagaa ggtcgcgccg aaatcggaga gcaagatttg cccgctgcgc ccgatcaaca
     5581 ggttacccgg tttcacgtcc tggtggatca aaccgttttc atgcacattg aacagggcat
     5641 ccgctgcctg cttgaggtag ggtaccagct cttccgcgta ggccaggcgg cgcgtgccat
     5701 ccttattacg gaacaggtcg aggatggtgc gcggcgcgta ctccatcacg agataggggc
     5761 gttcgtcttc cagcaggcca aagttataga ccggcaagat gttggggtgc tgcatagtag
     5821 caatgcggcg cgcctccgag aagaaactct cgcgcgcttt ctcgttatac tggtcaatgc
     5881 gcagcacttt gagggcaagc aggcggctga gcggcgcggg ttcgcgtacc tggtagacct
     5941 ggccatagct gcccgtgccg ataaacgaaa ccacttcgta aatgccgatg cgtgctccct
     6001 gtaccagaaa gtcgcagctc cgacatactt tcgccgaatc ggcgttctcg tgtcccccgt
     6061 tcgctcgtgg attcagacaa cgcgccaccg gtctatccta catctttcgt atagtcagaa
     6121 tactattgtt tttggacata atgaacaaac gggaattgca ccctcgcata ttatatctac
     6181 gctttcgttc gcgtgaagtt gcgtaaagcg cagaaaggta ctatgcatct acatccagcg
     6241 cgccgatgac cagcccaggt gcgcgtggct attgaccagc cgcaccgtcg cgtgttcacg
     6301 gtcgcgccag tcgatgacgc tgatggtagc cgggtccagg aagatgtggg aaggggtgga
     6361 tagcggcgcg cccagggcgg cgcagagcag cgctttgatc ggcccgacat gcgataccag
     6421 cgctacggcc tggtcggggt gcgcctgtga gagctgctct acggcatcgc aaatgcgctg
     6481 ttgtgttgcc gcgaggcttt cgccgcctgg aggggccagc gtcgcatctc gctcccatgc
     6541 ctctaactgc cgcgcatcct cgtcgc
//
LOCUS       DASZAK010000377         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_436101, whole genome shotgun sequence.
ACCESSION   DASZAK010000377 DASZAK010000000
VERSION     DASZAK010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436101"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VGT44_06625"
     CDS             <1..403
                     /locus_tag="VGT44_06625"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEV2580511.1"
                     /translation="SLAEQAIRVYQNGNLVRAFQVTTGQPDLPTPPGTWWVEGKKSPT
                     EFKSTEPPNSPNWYPPTPINYAMQFHSNGYYMHDSWWRTQYGPGTNFPHLDPGGTQYS
                     IHGSHGCVNMSKDDAAWLYSFVQVYTHIIIY"
     gene            complement(798..2210)
                     /locus_tag="VGT44_06630"
     CDS             complement(798..2210)
                     /locus_tag="VGT44_06630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2580512.1"
                     /translation="MPRIAIVGAGIAGLNAALTLHDAGLSCSIYEASNRVGGRMHSDA
                     TTWGDGMVSEWCGEFIDGNHETLWQLIERFGLKTINLGQGRLDRTQSVMYFCNRYYRA
                     DEMTRDFQAIYPILRRQVREVGFPTTHTHYTETGYRLDHLSVYDWIEQYVKGGHNTPL
                     GHMLDNACTGYFGLDSQAQSSLNLLSMFGPWASEERSSMPIRGSSKIAGGNEQLPQAI
                     ARSLPQGCIHLNHKLVALKRDGDDTLTLSFMTPDGSLEITCDHVILALPFSTLRHVDY
                     RQAGFDALKHTAITQLGYGTISKLILQFDTPYWYQDGPQTAWPRGNNGFILTDLDIQT
                     LWDTSIGQPGSHGLLVDYTSGHRGAAYMPPTPYTTTADSEHVQHYARRCLEQLELVFP
                     GISAYYTGRAALSYPTGDPHLLGSYACWRAGQYTGFGGYERVRQGPIHFAGEHCSVEF
                     QGFMEGGAREGERAAREVLK"
     gene            complement(2220..2456)
                     /locus_tag="VGT44_06635"
     CDS             complement(2220..2456)
                     /locus_tag="VGT44_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580513.1"
                     /translation="MSNIFRLLADEEKLTDELFAFITDPSADNRNERAIISVLHEVLP
                     QMVSVAECMILDGVDERHYVADYVQAVLEGETSE"
     gene            complement(2680..3570)
                     /locus_tag="VGT44_06640"
     CDS             complement(2680..3570)
                     /locus_tag="VGT44_06640"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2580514.1"
                     /translation="MSYQSGSPGEWGSMQAGPNISIANEELLGVDGCKHFLRSWITEG
                     RAVLLILHGLGGHSDWYIDMGNVMALRGITVYAMDHRGFGRSGGMAGDIDDYHTYVKD
                     IVLLLTEIRKRHPGGSIYLLGHSMGGLFATHVAAEHGELLAGILLLNPWIQDVSQLPF
                     LTAVTIFLSGLFKSKRYWQVAGGPEVMTTNPDAVHMLLVDSLWHRKQTARMLFQVVLM
                     RLAAFSKAREITLPALVMQAEADKAVNIATNQKLYEALASQQKTWKTYPGYMHDSEFE
                     HDRSQMDHDIATWLARQTEG"
BASE COUNT          817 a         1088 c         1050 g          838 t
ORIGIN      
        1 ttccctggca gagcaggcga ttcgcgtcta tcagaacggc aacctggtca gggccttcca
       61 ggtcacaacc gggcagcccg atctcccgac cccgccggga acctggtggg tcgaaggcaa
      121 gaagtcgccg accgagttta aatcaaccga gccgccaaat tccccgaact ggtatccacc
      181 gacgccgatc aattacgcga tgcagttcca cagcaacggc tactacatgc acgatagctg
      241 gtggcgcact cagtatggcc cagggacgaa cttcccccac ctcgaccccg gcggcacgca
      301 atactccatc cacggctcgc atggctgcgt gaacatgtcg aaagatgatg ccgcgtggct
      361 ctacagcttc gtgcaggtgt atacgcacat catcatctac taaatcgaag agcgcgggta
      421 tcctgttcgg atacccacaa cgtccctagt ttgagaaagc ggttctctcg taaatagaag
      481 ccatttcgac ttcgtggtgt tgaaaccaga atggttcctt cagatgtgtt ccttcgagtt
      541 caatgtggta gaaacagaac ggctcgtcaa cgtcactatg agggatagca tctacagtcc
      601 gaatcgttcc cttcagtcct ctgaaggggc tataactgac gacgcgcaca cgactgcctg
      661 ggggaaatac ctgattgttc atgcggaggg gaatgctcca aagatctagc ccgatgtggg
      721 atagtgggga catcatcttt tctttgtctt cacgaagcat accttctaaa aacgaaaaaa
      781 gcaacaaggg aactgtgtta tttcagaacc tcccttgcgg cgcgctcgcc ttcccgtgct
      841 ccgccttcca taaagccctg gaactcgacg gagcaatgtt cgccggcgaa gtggattggc
      901 ccctggcgca cacgttcgta gccgccgaac ccggtgtatt gcccggcgcg ccagcaggcg
      961 taactgccga gcagatgagg atcgcccgtc gggtagctca acgctgctct gccggtgtag
     1021 tacgcgctga tgccggggaa gaccagctcc aactgctcca ggcagcgacg cgcgtaatgc
     1081 tgaacgtgtt cggagtctgc cgtcgtcgta tagggcgttg gcggcatgta agccgctccc
     1141 cggtgtccgc tcgtataatc gaccagcagg ccgtgagagc ctggttgacc gatggaggta
     1201 tcccagagcg tctgaatatc caggtcggtg aggatgaagc cgttgttacc gcgcggccag
     1261 gcggtctgcg ggccatcctg gtaccagtag ggcgtatcga actgcaaaat gagcttggag
     1321 atagtgccgt agccaagctg cgtgatggcg gtatgcttca atgcgtcaaa cccggcctgc
     1381 cgataatcga cgtgtcgcag ggtactgaac ggcagggcga gaataacatg gtcgcatgtg
     1441 atttccaggg aaccatcagg ggtcataaac gacagcgtga gcgtatcatc cccgtctcgt
     1501 ttgagcgcca ctagcttgtg gttcaggtgt atgcaccctt gcggcaggct gcgggcaatc
     1561 gcctgcggca gttgctcgtt gccgcctgcg attttgctgc ttccccgtat cggcatgctc
     1621 gaacgttcct ccgaggccca cgggccaaac atggagagca ggttgagcga actttgcgcc
     1681 tgcgaatcca gcccgaagta gccggtgcag gcattatcca gcatatgtcc gagcggtgta
     1741 ttatgtccgc ccttcacgta ctgctcgatc cagtcgtaca cactcaggtg atcgagccgg
     1801 tagccggttt cggtatagtg ggtatgggtc gtggggaaac caacctcgcg tacttgccgc
     1861 cgcaggatgg gatagatggc ctggaaatcc ctggtcatct cgtctgcacg atagtagcgg
     1921 ttgcagaaat acatcacgct ttgtgtccgg tcgagccttc cctgccccag gttgatggtt
     1981 ttgagaccga agcgttcgat aagctgccag agcgtctcgt gattaccgtc gatgaattcg
     2041 ccgcaccact cgctgaccat gccgtcgccc caggtagtcg catcagagtg catacgccct
     2101 ccaacgcggt tggacgcttc atagatgctg cacgagagac cggcatcgtg caaggtcaac
     2161 gcggcgttaa gccctgcaat acctgcgccg acgatagcaa tgcgtggcat cgcgccccct
     2221 cactcggatg tttcgccctc gaggacggcc tgcacataat cggcgacata gtgccgttcg
     2281 tccacaccat ccaggatcat gcattcagcg accgagacca tctgaggcag cacctcgtgc
     2341 aacacactga taatcgctcg ttcgttgcga ttgtccgcgc tggggtcggt gataaacgcg
     2401 aaaagttcat cggtcagttt ttcttcatcg gcaagcaaac ggaaaatgtt ggacactgct
     2461 tcagcctttc atcgtgatcc tcattgaaaa cgagtacgtc gtcctgcttt ggggttttat
     2521 ctacttgcac aatcttgtaa tacaaagata cctgccaggg agattcaatg tcaagctaaa
     2581 gcaacgacgc ggatgtataa agtatctgta ggagcacggt aaaccggctc ctacaggggg
     2641 ttggttgcct acaagtattt cgtaccccca atgtttccct cagccctccg tttgccgggc
     2701 aagccaggtt gcgatatcat ggtccatctg tgagcggtcg tgttcgaatt cgctatcgtg
     2761 catatagccg ggataggttt tccaggtttt ctgctggctg gcaagcgcct cgtacaattt
     2821 ttgattggtg gcgatattga cggctttgtc agcctccgct tgcatcacca gcgcggggag
     2881 ggtaatctcc ctggctttcg agaaagcagc gaggcgcatc aggacgacct ggaacaacat
     2941 acgggccgtt tgcttgcgat gccagagcga atcgaccagc agcatatgaa cagcgtccgg
     3001 gttggtcgtc atcacctctg gtcctccagc gacctgccag taacgcttcg atttgaagag
     3061 gccgctcagg aagatcgtca ccgccgtcag gaacggcagc tggctcacat cctgaatcca
     3121 ggggttgagg agcaggatgc cggcaagcag ttcgccatgc tcggcggcaa cgtgcgtggc
     3181 gaacaggccg cccatgctgt gtcccaaaag gtagatactg ccgccgggat ggcgcttgcg
     3241 aatctcggtc aggagcaaaa caatatcttt tacataggtg tggtaatcgt cgatatcgcc
     3301 tgccatgcca cctgagcggc caaacccgcg atgatccatg gcgtacacgg tgatgccacg
     3361 caaggccatc acattgccca tatcgatata ccagtcgctg tggccgccca atccatgcag
     3421 gatgagcagg acggcccggc cttccgttat ccaactgcgc aggaagtgct tgcatccatc
     3481 gacgcccaac aactcctcgt tggcgatgct gatgttcggt cctgcctgca tactccccca
     3541 ctctccgggg gaacctgact ggtaactcat gtcgatctcc ttctcgcctg ggatgcatca
     3601 cttgcagcat gatgaaagct cacaaccaga gtcggtaatg tcatcccttc gatgttctca
     3661 gggcaacaac ctctgctcgg cattgtaatg tcatccttcg ctgcgctcag gatctgccga
     3721 cctatccgcc gagacccttc gctgcgctca gggtgacatt actgaacatg gtcgttaaca
     3781 ttcattaagc tgt
//
LOCUS       DASZAK010000378         3131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_436121, whole genome shotgun sequence.
ACCESSION   DASZAK010000378 DASZAK010000000
VERSION     DASZAK010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436121"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..2477)
                     /locus_tag="VGT44_06645"
     CDS             complement(111..2477)
                     /locus_tag="VGT44_06645"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEV2580515.1"
                     /translation="MATHVFSHGLGEAYKRKEDARFIRGQGNYVDDVNLPGMLYGDIV
                     RSPYAHALIKNVDISEAMKVPGVLAVITGKDLDAAGLAWMPTLSADTEAVLALDRVHY
                     QMQEVAFVVATSRYAAADGAAAVEVDYEPLQVLVDAKKALDADAPLLRPDKKEKQPTN
                     QIFHWETGEREATDAAFAEAAANGVVVKQEMYIPRIHPAPIETCGCVADYHKATGKLT
                     VWMTSQAPHVVRTLFAIVAGLPEHRIRIISPDIGGGFGNKVPIYPGYVCAVVASLTTG
                     KPVKWIEDRTENLSSTGFGRDYHIKAEVAADKDGTVQALRVYTLADHGAFDAAANPSK
                     FHAGLFHICTGSYDFKHAFVEVDGVHTNKAPGGIAYRCSFRVTEASYLIERAMDTLAH
                     NIGKDPAELRMQNFIKPEAFPYRSALDWTFDSGNYEGALKLAMKNVDYEGLRREQAEK
                     RARGELMGIGISSFTEIVGAGPGKHFDIAGIQMFDSCEIRVHPTGKAIARIGVQTQGQ
                     GHETTFAQIIAAELGMSPDDIDVEHGDTDTAPYGLGTYASRSTPVAGAATALAARKVR
                     DKAKKIAAYLLEVGEDDLEWEPGRFFVKGAPGKGKTIQELAFAAYTNCPPYLEPGLEA
                     VNYYDPPNLTFPFGSYICIVDIDRGTGQVHIRRFMAVDDCGTIINPMIVEGQIHGGLT
                     QGLAPAMYEELSYDADGNVLGGNFQDYLIPTSMETPHWETDRTVTPSPHHPIGAKGVG
                     ESPTVGAPQAIVNAVVDALAFTGVRHIDIPLSPWKVWQVLKEKGLTAD"
     gene            complement(2477..2989)
                     /locus_tag="VGT44_06650"
     CDS             complement(2477..2989)
                     /locus_tag="VGT44_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2580516.1"
                     /translation="MSTHHISVTINGTPYEADVEARTLLVHWIRDTLGLTGTHIGCDT
                     SACGACTILVDGKSTKSCTMFAVQANGRQLMTVEGLEQNGQLHPLQEGFHEEHALQCG
                     YCTPGMMMTSFALLQRNPNPTEHEIRVGISGNLCRCTGYVNIVKAVQFAAKKMAAAEE
                     VPVASGQGGE"
     gene            complement(2991..3119)
                     /locus_tag="VGT44_06655"
     CDS             complement(2991..3119)
                     /locus_tag="VGT44_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580517.1"
                     /translation="MAAAASRPTSDTRGPAEYKRDMVRTLTVRALRKAHARATGGQ"
BASE COUNT          558 a          973 c          937 g          663 t
ORIGIN      
        1 cgtacctgtt gcatacgcga ttcggttgcg ccaccgggta cgcgggcgac gcaagcgtcc
       61 ccacaccgcc ccacatccgc ccccgcccct acggacggat gatggttgtg ttagtctgcc
      121 gtcaggccct tttccttcaa cacctgccag accttccagg ggctgagggg gatgtcgatg
      181 tggcgcactc ctgtaaatgc cagggcgtcg acgacggcgt tgacgatggc ctgcggcgcg
      241 ccgacggtgg gtgactcgcc cacgcccttc gcgccgatgg gatggtgcgg cgatggggtg
      301 acggtacggt cggtttccca gtgcggcgtc tccatcgagg tcgggatcag gtagtcctgg
      361 aagttcccgc ccaacacgtt gccatcggcg tcatacgaca gttcttcata catggccggg
      421 gccagtccct gcgtcaagcc accgtgaatc tgcccttcga cgatcatcgg attgatgatc
      481 gtgccgcagt cgtccaccgc catgaagcgg cgtatatgca cctggccggt tccccgatcg
      541 atgtccacga tgcagatgta gctgccgaaa ggaaaggtca gattgggcgg gtcgtagtag
      601 ttgactgctt ccaggcccgg ctccaggtat ggcgggcagt tggtgtaagc cgcgaatgcc
      661 agttcctgga tggtcttgcc tttgcccggc gcgcctttga cgaagaagcg acccggctcc
      721 cattcgaggt catcttcgcc gacttcgagc aggtaggccg cgatcttttt ggctttgtcg
      781 cgcaccttcc tggcggccaa cgccgttgcc gcgcctgcca cgggcgtact gcggctggca
      841 taggttccca ggccataggg cgcggtatcc gtgtcgccat gttcgacatc gatatcatct
      901 ggagacatac cgagttccgc cgcgatgatc tgcgcgaagg tggtctcgtg accctggccc
      961 tgcgtctgca cgccgatgcg cgcgatcgcc ttgccggtgg gatgcacccg gatctcgcag
     1021 ctatcgaaca tctggattcc ggcgatatcg aaatgcttgc ctggcccggc tcccacgatt
     1081 tctgtgaagc tggagatacc aatgcccatc agttcgccgc gcgcgcgttt ctcagcctgt
     1141 tcgcgccgca atccctcata gtctacgttc ttcattgcca gtttcaacgc gccctcatag
     1201 ttgccgctat caaacgtcca gtcgagcgcg gaacgatagg gaaaggcttc cggcttgatg
     1261 aagttttgca ttcgcagttc cgccgggtcc ttgccgatgt tatgcgccag cgtgtccatg
     1321 gcgcgttcga tcaagtacga cgcctcggtg acgcggaacg agcagcgata ggcgatgccg
     1381 cccggcgcct tgttagtatg caccccgtct acctctacaa aggcatgctt gaagtcatac
     1441 gagccggtgc aaatgtggaa caggccagca tggaatttgg aagggttagc cgccgcgtca
     1501 aacgcgccat ggtcggccag ggtatagacc cgcagcgcct ggacggttcc atccttatcg
     1561 gcggctacct ccgccttgat atggtagtcg cgcccaaagc cggtactgct cagattttcg
     1621 gtgcggtcct cgatccactt gacgggtttg ccagtcgtca gagaagccac aactgcgcac
     1681 acgtagccgg gatagatggg aaccttgttg ccgaagccgc cgccgatatc cggcgagatg
     1741 atacgaatgc gatgttccgg cagacctgcc acaatcgcaa acaaggtgcg gaccacgtgc
     1801 ggagcctgcg aagtcatcca gaccgtcagc ttgcccgtcg ccttatgata gtcggcgacg
     1861 cagccgcagg tctcaatcgg cgcgggatga atgcgcggga tgtacatctc ctgtttgaca
     1921 accacgccat tggccgctgc ctcggcaaaa gcagcatcgg tcgcctcgcg ctcgcccgtt
     1981 tcccagtgaa aaatctggtt ggtgggctgc ttttcttttt tatcggggcg cagcagtggc
     2041 gcgtcggcat cgagcgcttt ttttgcatca accagcacct gcagcggctc gtaatcgacc
     2101 tcgacggctg ccgcgccatc ggcggcggcg tagcggctag tggcgacgac gaaagcgacc
     2161 tcttgcatct ggtaatgcac gcgatccagc gccagcaccg cctcggtatc ggcggagagc
     2221 gtcggcatcc aggccaggcc ggcggcatcc aggtctttgc cggttatgac ggccagtacg
     2281 cccggcacct tcatcgcctc gctgatatca acgttcttga tgagggcgtg cgcgtaaggg
     2341 ctgcgcacaa tatcgccata cagcatcccg ggcaggttta catcatcgac ataattgccc
     2401 tgtccccgga tgaagcgcgc atcttctttg cgcttgtacg cttcgcccag cccgtgcgag
     2461 aaaacatgcg tcgccattat tcgcctcctt gcccggacgc caccgggact tcttcagcag
     2521 ccgccatctt cttcgccgcg aattggactg ctttgacgat attgacgtag ccggtacaac
     2581 ggcacaggtt gccagaaatg ccgacacgaa tctcatgctc ggtcgggttc ggattgcgct
     2641 gtaagagcgc gaacgaggtc atcatcatgc ccggcgtaca gtagccgcat tgcaacgcat
     2701 gttcctcgtg gaagccttcc tgcaggggat gcagctggcc attttgctcg aggccctcga
     2761 ccgtcatgag ctggcggcca ttggcctgaa ccgcgaacat cgtgcatgac ttggtcgatt
     2821 ttccatccac caggatcgta cacgcgccac acgcgctggt atcacagccg atatgcgtac
     2881 cggtcaagcc taacgtatcg cgtatccagt ggacaagcag cgtccgcgcc tcgacatcgg
     2941 cctcgtaagg tgtgccattg atagtgacag agatatgatg ggtactcatg ctattgtcct
     3001 cctgtcgctc gcgcgtgagc tttgcgcagg gcgcgcaccg tcagtgtgcg caccatgtcg
     3061 cgcttgtatt ccgccgggcc gcgcgtatcg ctggttggcc gggacgcggc ggcggccagg
     3121 tcggcagcag c
//
LOCUS       DASZAK010000379         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_400351, whole genome shotgun sequence.
ACCESSION   DASZAK010000379 DASZAK010000000
VERSION     DASZAK010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400351"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VGT44_06660"
     CDS             <1..1249
                     /locus_tag="VGT44_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV2580518.1"
                     /translation="ECLDQVVERSEFHTNTLAAAANLHASNGSLQYQPAIKRGLGIAA
                     SAYICGAGKPIYWNDLPHSAVQIRLDRGGGVTVYCGATDIGQGSTSILAYIVAEELGI
                     QPERIHLETADTTLTPVDLGSYSSRVTFMAGNAAIAAARKLKEQLFEVASERLHVPSE
                     SLQAANDVIFDEHDPGVALPFEHAVQFAEARFGALVAAGSYAPPEGIHGDYKGAGVGP
                     SPAYSYSACVAQVAVDVETGELVVEKLWLAHDVGQSINPLLVAGQVEGGAYMGCGEAV
                     MEQQIFRKGLHKIPSLLDYKLATTLDTPEIETILVEIPDREGPFGAKEAGQGPLNPVI
                     PAIANAVYDAVGVRIDEVPITSDKVFKALKNLAQGKGTELQPTPIELSSPWPTRLISW
                     QPNGSQGEDQSKASHVPVQGGVS"
     gene            1249..>2013
                     /locus_tag="VGT44_06665"
     CDS             1249..>2013
                     /locus_tag="VGT44_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV2580519.1"
                     /translation="MLRLPPFRYLAPRHLDEAAQMLEQEGEQAMLVAGGTDLYPNMKR
                     RQFTPPVLIGLRAIPELKTISGSPEQGMRIGAGVTLTRLAEHPALLEHYPALATAAGS
                     VSTPQLRNAGTIGGNLLLDTRCNYYNQTEFWRRSIGYCMKKDGDVCLVAPGSPRCWAI
                     SSADTAPVLVSLGASVRLVSARGERVIPVHELFRDDGMYPYTKAADEILTEVILPPAA
                     GWQSVYLKLRRRGSFDFPILGVAVALRPAGDGSIADA"
BASE COUNT          411 a          623 c          559 g          420 t
ORIGIN      
        1 tgaatgcctc gatcaagtcg ttgagcgctc cgagtttcat acgaacacat tagctgcagc
       61 agcaaatctt cacgcatcaa atgggagctt gcagtaccag ccggcgatca agcgcggcct
      121 gggcatagct gcttcagcgt acatctgtgg cgctggcaag ccgatctact ggaacgacct
      181 gcctcactcc gccgtgcaga tcaggctcga ccgtggcggt ggtgttaccg tctactgtgg
      241 cgctaccgac attgggcagg gttcgacctc gattcttgcc tatatcgttg ccgaagagct
      301 gggtatccag cccgaacgca ttcacctgga gaccgccgac acgaccctta cgcccgtcga
      361 tcttggctca tattccagtc gtgtcacttt catggcgggc aatgctgcta tcgccgccgc
      421 ccgcaaatta aaagaacagc tcttcgaagt cgcgtccgag cgcctgcatg tgccctctga
      481 aagcctgcaa gccgccaatg atgttatctt cgatgagcat gaccccggcg tagccttgcc
      541 ctttgagcac gccgtgcaat ttgccgaggc tcgttttggc gccctggtcg ctgctggctc
      601 gtatgctcct cccgagggta ttcatggcga ttacaaaggc gctggcgttg gtccttcgcc
      661 cgcctattcc tactcggcct gcgtagcaca ggtagcagtt gacgtagaaa cgggcgagct
      721 ggtggtggaa aagctctggc tcgcgcatga cgtgggacag tctatcaatc ccttactcgt
      781 agcgggccag gtcgaaggcg gcgcctatat gggctgcggg gaggctgtca tggagcagca
      841 aattttccgc aaaggactac acaagatacc atctctgctg gactacaaac tggccacaac
      901 gctggacacc ccagagatcg aaaccatcct tgtcgaaatc cccgaccgcg aaggcccatt
      961 tggggcaaaa gaagcgggcc aggggcctct gaacccagtt atcccggcca ttgccaacgc
     1021 tgtctacgat gccgtaggtg tacgcatcga cgaggtaccg attacatctg acaaggtttt
     1081 taaagctttg aaaaacctgg cacaagggaa ggggacggaa ctccagccca cgcctattga
     1141 actttcttct ccctggccca ctcgccttat ctcctggcaa cccaatggga gccagggaga
     1201 agatcaaagc aaggcaagcc atgttcctgt gcagggagga gtctcctgat gttacgtttg
     1261 ccaccatttc gttacctggc tcctcgccac ctcgacgagg ccgcgcaaat gctagaacag
     1321 gaaggcgagc aggccatgct ggtcgctggc ggtaccgatc tctacccaaa catgaaacgc
     1381 cgccagttta ccccaccggt gctgattggc ctgcgcgcta ttcccgagtt aaaaaccatc
     1441 agcggctccc ctgagcaggg tatgcgcata ggagctggcg ttaccctcac ccggcttgct
     1501 gagcatccgg cgctgctaga gcattatccg gcgctggcta ctgctgctgg ctcggtctcg
     1561 acaccgcaat tgcgtaacgc cggcactatc ggcggcaacc tgctgctcga cacacgctgc
     1621 aactactaca accagaccga gttctggcgc cgctcaatcg gctactgcat gaagaaggac
     1681 ggcgatgttt gcctcgtggc ccctggcagc ccgcgctgct gggccatctc ttcggcagat
     1741 accgcaccgg tactggtcag cctgggtgca tccgtgcgcc tggtgagcgc gcgaggcgag
     1801 cgcgttatac ccgtacacga actgttccgc gatgatggca tgtatcccta caccaaagca
     1861 gcagacgaga tactcaccga ggtcatcctt ccccccgccg ccggctggca aagcgtctac
     1921 ttgaagctgc gccggcgcgg ctcattcgac ttccctattc tcggtgtcgc agtagcattg
     1981 cgcccggccg gggatggttc tattgctgat gca
//
LOCUS       DASZAK010000380         3518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_506928, whole genome shotgun sequence.
ACCESSION   DASZAK010000380 DASZAK010000000
VERSION     DASZAK010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506928"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="VGT44_06670"
     CDS             <1..1149
                     /locus_tag="VGT44_06670"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580520.1"
                     /translation="KLTGSAINLGISSASNFLPYLLFGLILGAWTDRVNRKLMMIFAD
                     IARAMIVASIPLLAVLGALPVWWIYVVGFLHSTLTICFEAGQFAAIPSLVNQDDLVTA
                     NGRIQASYSAAQIAGPLLAGLLVAIVALPSLMLIDALSFLVSSFSLALIRISFNVKTG
                     EQRAKTSIRHDVVEGLRYVLSHPVLRNISMMMAMVNFVSTASYAQLVLFAKVRLAASD
                     GQVSLLFSAASLGVVIMGLLAGPLRKRWSFSTVALGALMLEGLLGVVFSLMRWYWIAA
                     ILWMLIGGIGILFNINTGSLRQAIVPNHMLGRVISIASVLAWSAIPLGSLLGGFAISW
                     THNVALVFAITGTLTFLIPFCFSFTALGHADRYIPKKEEQASEYAPTS"
     gene            1270..2073
                     /locus_tag="VGT44_06675"
     CDS             1270..2073
                     /locus_tag="VGT44_06675"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV2580521.1"
                     /translation="MYRLLAIDLDGTLLTPRPHKHITVRAKLALRRAVDAGMTLVIAT
                     GQNLAVLQNTCGDLQLNGPQIIENGAVIADLHGHIYHEHYLPTEHVLPALELLRAFGF
                     YRAYHTIHRVYVDKNTPRAREWYRPPVPPVIEVANVAALHPLPCVKVVGIGAEERIRA
                     LRDSFEQRLAGKLYVTQSAFDLVEFLHPDVSKGHALQQIAADLGIVPEDVVAFGDNHN
                     DIGMLRFAGLGVAMGNAHDEVKAEADYVTLSNAEDGVAVAIEEMILPTL"
     gene            2158..3093
                     /locus_tag="VGT44_06680"
     CDS             2158..3093
                     /locus_tag="VGT44_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580522.1"
                     /translation="MTDAIIRTEHLSRFFGSVKAVDDLSLEVPAGIVFGFLGPNGAGK
                     TTTIHLLLGLLEPMHGQASVLGFDTRTQADEIRARIGALLEFAGLYERMSAEDNLDFY
                     GRIYHMPAPERRARIKELLTHLELWDRRADQVGKWSRGMKQKLAVARALFHHPPLIFL
                     DEPTAGFDPIAAAALRNDLASLVAREGVTVFLTTHNLSEAEKLCAQVGVIRQGKLVTV
                     GNPDELRLRTGGPQAEIIGHGFNEQTLTLLRERPEVANAELHNSHLTIELRGESEIGP
                     LVSLIVRSGGEVEEVRRGKASLEDVFLTLMEEENK"
     gene            3102..>3518
                     /locus_tag="VGT44_06685"
     CDS             3102..>3518
                     /locus_tag="VGT44_06685"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580523.1"
                     /translation="MTMIWKESKDSLFQGGWRALIRPLFVIAIIGVYLPLQFGSQWVE
                     LTPLGMVLLLWVPFFVIISFIGDAIAGERERHTLETLLASRMPDRAILLGKIMATVLF
                     AWGMSILSILLGLVLVDLFRTQGTWKFYPLNLFVDAI"
BASE COUNT          736 a          964 c          989 g          829 t
ORIGIN      
        1 aagctcaccg gttccgccat caacctgggc atttccagcg cgtcaaactt cctgccctac
       61 ttgctcttcg ggctgattct gggtgcgtgg acagatcgtg taaatcgcaa gctcatgatg
      121 atcttcgccg atattgcgcg cgccatgatc gtggcaagca tcccactgct ggcggttctg
      181 ggtgcgctcc ctgtctggtg gatctacgtc gtgggcttcc ttcactcaac gctaaccatc
      241 tgctttgaag ccgggcaatt cgcggctata cccagcctgg tcaatcaaga cgaccttgtt
      301 accgccaatg ggcgcatcca ggccagctac tcagctgctc agatcgcagg cccgctgctg
      361 gccggtttgc tggtggctat tgtagcgctg ccgtcactta tgttgattga tgcgctatca
      421 ttcctggtct catcgttttc gctcgccctc atccgcatca gtttcaatgt gaagacaggc
      481 gagcagcgag ctaagaccag cattcgccat gacgtggttg aaggattgcg ctatgtattg
      541 agccacccgg tgctgcgcaa tatctcgatg atgatggcga tggtcaattt tgtaagcacg
      601 gcctcttatg cccagcttgt actattcgcg aaagtacgcc tcgcggcaag cgatggtcag
      661 gtaagtttgc tcttctcggc agccagtctt ggcgtggtga ttatgggtct gctagccggg
      721 ccactgcgca agcgttggtc gttcagcacg gtggcattag gagcgctgat gctggagggt
      781 ctgctgggtg ttgttttctc gctgatgcgc tggtactgga tcgcagctat cctctggatg
      841 ctcatcggtg gaattggcat tctctttaac atcaatacgg gcagcctgcg tcaagccata
      901 gtcccgaatc acatgctggg tcgcgtcata agcatcgcca gcgtgctggc ctggtccgcc
      961 attcctcttg gcagcttgct tggtggtttc gccatctcct ggacgcacaa tgttgccctg
     1021 gttttcgcca tcaccggcac actgacattc ttgatcccat tctgcttctc ctttaccgcg
     1081 cttggacacg ccgaccgcta cattccgaaa aaagaggagc aggcgtccga atacgctccg
     1141 acttcataaa gtcacaacta aattgttcaa ttccattgtc tgcttgccaa accaacccct
     1201 ccctgtttat acttgaatta tcactggaaa gcacaactgg ttcgtccctt ctattcagga
     1261 gcatctcgcg tgtatcgcct gctggcaatc gacctcgatg gcactctgtt gaccccacga
     1321 ccgcacaagc atatcaccgt gcgcgcgaag ctggccttgc ggcgagcggt tgatgctggc
     1381 atgacgctgg tgatcgccac cgggcaaaac ctggcggtgt tgcaaaacac ctgcggggat
     1441 ttgcagctca acgggccgca gatcatcgaa aacggcgcgg tcatcgcgga cctgcatggg
     1501 catatctatc atgaacacta tctccctacg gagcatgtcc tgcccgcgct ggagctattg
     1561 cgcgcgttcg gtttctatcg cgcctatcat acgatccacc gcgtgtatgt cgataaaaat
     1621 acaccgcgcg cgcgtgaatg gtatcgaccg ccggtgccgc ccgtgatcga ggttgcgaat
     1681 gtcgccgctc tccatccatt accgtgtgtc aaagtcgttg gtatcggggc cgaagagcgc
     1741 atccgcgccc tgcgcgattc cttcgagcag cggctcgcgg gcaaactcta cgtcacgcaa
     1801 tccgcgttcg acctggtcga attcctgcac ccggatgtat cgaagggtca cgcgctgcaa
     1861 cagatcgcgg cagacctggg aatcgtaccg gaagacgtgg ttgcctttgg ggataaccat
     1921 aacgacatcg gcatgttgcg cttcgccggc ctgggcgttg cgatgggcaa tgcgcacgac
     1981 gaggtgaagg ccgaggcgga ttatgtcacg ttgagcaacg ccgaagatgg cgtggctgtg
     2041 gcgatcgagg agatgatttt accaacatta tgaaaacggg aacgtacatt aagatagtat
     2101 attctgtgag tgattcataa ctgagcaata cttcgtagag aaaggaattg tcatgacatg
     2161 acagatgcta tcattcgcac agaacatctc tcacgttttt ttggttcggt caaagccgta
     2221 gacgatctct cgttggaggt gccagcaggc atagtctttg gtttcctggg gccaaatggc
     2281 gctggcaaaa cgaccactat tcacctcctg cttggcctgc tggaaccgat gcatggccag
     2341 gccagcgtac tcggcttcga tacccgcaca caggcagatg aaattcgcgc acgaatcgga
     2401 gctttactcg agtttgcagg gctttacgag cgcatgagcg ccgaagacaa cctggacttt
     2461 tacgggcgta tctaccatat gcccgccccc gaacggcggg cgcgcattaa agaactgctc
     2521 acccacctcg aactctggga ccgccgcgcg gatcaagtag gcaagtggag caggggcatg
     2581 aaacaaaaac tcgcggtagc gcgtgcgctc tttcaccatc ccccgctgat cttcctggat
     2641 gagccgacag cagggtttga tccgatagcg gcggcggcgt tgcgtaacga cctggcaagc
     2701 ctggtagcgc gcgaaggggt gacggtcttc ctcactacgc acaatctgag cgaggccgaa
     2761 aagctctgtg cccaggtggg agtgattcgc cagggaaaac tggtgacggt tggcaaccct
     2821 gatgaattgc gtttgaggac aggtggcccc caggcagaga tcatcgggca tggcttcaac
     2881 gagcaaacac tgacattact gcgagagcgg ccagaggtag cgaacgctga actgcacaat
     2941 agtcatttga ccattgagct gcgaggagaa agcgaaattg gtccgctcgt cagcttgatt
     3001 gtgcggtcag gcggggaggt tgaggaagtc cgcaggggca aggccagcct ggaagatgtt
     3061 ttcctgacac tgatggagga agaaaacaaa tgagcgacct catgaccatg atatggaaag
     3121 aatcgaagga ctccctcttc cagggaggat ggagagcatt gatacgccct ctgttcgtga
     3181 tcgctattat cggagtctat ctcccattgc aattcgggtc tcagtgggtg gaactcacac
     3241 cacttggaat ggttcttctg ctgtgggtcc ccttcttcgt gattatcagt ttcattggtg
     3301 atgccatcgc cggagagcgc gagcggcata cactggaaac cttgctggcc agtcgcatgc
     3361 cagatcgggc gattctgctg gggaagatca tggctaccgt cctatttgcc tggggcatga
     3421 gcatacttag tatactgctc gggctggtct tagttgacct gtttcgtacc caggggacct
     3481 ggaagttcta tcctctcaat ctattcgtgg atgccatc
//
LOCUS       DASZAK010000381         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_80921, whole genome shotgun sequence.
ACCESSION   DASZAK010000381 DASZAK010000000
VERSION     DASZAK010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80921"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VGT44_06690"
     CDS             complement(<1..450)
                     /locus_tag="VGT44_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580524.1"
                     /translation="MEKTHDFAQQLADELRQFPDIHDWSIRELNEHSWQLFITNQHED
                     TRREVRTARLTVQIFHDFPQSDPPLRGSASFTLVPSPTLRADAQRMIDQSLLMAKIVG
                     NPSYTLPLPPAQGYPVVQSCDNEIVERPREVLDELFTTINATIGTEEQ"
     gene            complement(450..1832)
                     /locus_tag="VGT44_06695"
     CDS             complement(450..1832)
                     /locus_tag="VGT44_06695"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEV2580525.1"
                     /translation="MNATTVQTIQQLVSDLEKIFPYASALWTTTEGTRASLNQKDRSL
                     NALDPDSGVVFTVFTGTSFEEFAISEAESAELAQSVKRWASDLPQRQKAAKPFALQEN
                     SESKTFVTPVEIDPATLPAHVKFEMLQSLQAHLQGLDRRVINAIVGYGDSSTRSVFLR
                     PSYTLDQTLKRTIIFLELMVRDDRQMQYAYMPHSGTGGFEITRFSDEELDRLKEDALH
                     LLQAEPIPPGDYEVVADNSTAGVIAHECFGHGVELDLFPKKRAKSAQYIGKRVAAPGV
                     NMFDDPAYPGAYGSYFFDDEGMLAQPTQILEDGILINPLTDLSSILALPTFRRTANGR
                     RESFARKAYARMSNTFFGAGSHSSSDMLEELEDGIYLRNALSGMEDPLGWGVQVTIHI
                     GEEYKHGKPTGRIFSPVGISGYVPTLLSSISAIGHDLALDGGFCGKGQKELIPVTTGG
                     PHLRLRARLG"
     gene            complement(2195..>3020)
                     /locus_tag="VGT44_06700"
     CDS             complement(2195..>3020)
                     /locus_tag="VGT44_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV2580526.1"
                     /translation="LSEHPLGRAVVQAAIAREVTVPDPEEFTALPGLGVRVRIEGGEV
                     VIGRPRLLFEQGITIDPGIAARVKNLASDGRSIILVARNGQVIGMLALEDTLRPEASQ
                     VITRLKKQGIRTILVTGDNVVTAERIAGELGISDIHAEVLPAQKVEIVKQLQAQGLNV
                     AFVGDGVNDGPALATANVGVAMGLGGTDVAIETAEIALLSDDLAKLPHLLSLSRQAMR
                     AIKQNLVFSLGVLAIAVGLAIPGILAPVTGALLHELSSIPVIANSARLIGIKERAE"
BASE COUNT          638 a          821 c          870 g          691 t
ORIGIN      
        1 ctgttcctct gtgccgatgg tggcgttgat ggttgtgaag agttcgtcta gcacttctcg
       61 cggtcgctcg acaatttcgt tgtcacagct ttggacgacg ggatagccct gagctggtgg
      121 cagtggaagc gtgtaggagg ggtttcccac gattttagcc attagtaggg actgatcgat
      181 catgcgctgg gcgtcggcgc gtagtgtagg agacggcacg agcgtaaagc tggcgcttcc
      241 ccgcagtgga ggatcgctct gggggaaatc atggaagatt tgaacggtga gacgcgccgt
      301 gcgcacttcc cggcgcgtat cctcgtgttg attggtaatg aagagttgcc agctatgctc
      361 gttcagttcg cgaatagacc aatcgtgaat atcgggaaac tgccgcaact catcggcgag
      421 ttgttgcgca aaatcgtgtg ttttttccat cagccaagcc tcgctctcaa acgtaaatga
      481 ggaccgccgg ttgttaccgg aatcaactct ttctgaccct tgccgcagaa tcccccgtca
      541 agcgccaggt cgtggccgat ggcgctaatg gagctgagca gggttgggac gtagccgcta
      601 atgccgaccg gcgaaaatat gcgcccggtc ggcttgccat gtttatattc ttctccgatg
      661 tgtattgtga cctggacgcc ccacccgagc gggtcttcca tgccgctcag tgcattgcgc
      721 aagtaaatgc cgtcttccaa ttcctcgagc atatcggagg aggagtgcga gccggcgcca
      781 aaaaatgtat tggacatgcg cgcgtaggct ttacgcgcaa aactttcccg ccgaccattg
      841 gcggtacgcc ggaaggtagg cagcgcgagg atggaagaaa gatcggtgag tgggttgatt
      901 agaatgccat cttcaagaat ttgcgttggt tgggccagca tgccttcatc gtcgaagaag
      961 taggagccgt atgcgccggg ataggccggg tcgtcgaaca tattgacgcc aggggcggcc
     1021 acgcgcttcc caatgtattg agccgatttt gcgcgctttt tggggaaaag atcgagttcg
     1081 acgccgtgac cgaaacattc gtgagcaatc accccggcag tcgaattatc tgctaccacc
     1141 tcgtagtcgc cgggaggtat tggctcggct tgaagcaggt ggagggcatc ctctttcaga
     1201 cgatccagct cctcgtcgct gaagcgagtg atttcaaacc cgcctgtgcc gctatgtggc
     1261 atatatgcat actgcatctg ccgatcgtcg cgaaccatca gttcgaggaa gatgatcgtg
     1321 cgcttgagcg tctggtcgag ggtataggag gggcgcaaaa aaacgctccg cgtggaacta
     1381 tcgccgtagc ccacaatggc gttgatgaca cgccggtcga gtccctgaag atgtgcttgc
     1441 agggattgca acatctcaaa tttcacatgc gcaggcagtg tcgcaggatc gatctctacc
     1501 ggcgtgacga atgtcttact ctcgctgttc tcttgcaggg cgaatggttt tgcggccttc
     1561 tgtcgttggg ggagatcgct cgcccagcgt ttcacggatt gtgccagttc cgcgctctca
     1621 gcctcgctta tagcaaattc ttcaaaggaa gtcccggtga aaacggtgaa gacgactccc
     1681 gaatcgggat caagggcgtt aagggagcgg tccttttgat tcaaggaggc tcgtgtgcct
     1741 tctgtcgtgg tccagagggc agaggcatag ggaaaaattt tttcaagatc cgacaccagt
     1801 tgctgaatgg tctgaacagt cgttgcgttc acgattggcg cccttcctta acatttcgta
     1861 tggtctggca gtctgttggt gtgtccaacg cttggggtat gtataaaagg gtatcacttt
     1921 tctcctgcat tatccagtgc gagttggcac aatgccgaaa gaaagcaccc tccttgtagt
     1981 ggcgcgatga ataaatttaa aagctgaaga gggtgtaagc ctctcgcata accaggacga
     2041 ccacatcgtt tccgacactg cctacaacca ctactcgatc ctggacacca tcgaggatag
     2101 ctggcatctt ggctgcctgg gcttcacttg cgataccgtc aacgttcccg acatgcgcgc
     2161 atttctggag gactgaacca gggtgactat cggctcactc ggcgcgttcc ttgatcccaa
     2221 ttagacgcgc cgagttggcg atcaccggaa tggaagagag ttcgtgcaag agcgctccgg
     2281 tcaccggagc caggatgcct ggaatggcta gccctaccgc aatcgccagg acgcccagcg
     2341 aaaaaacgag attctgcttg atggcccgca tggcctgtcg ggaaagactc agcaggtggg
     2401 ggagcttcgc caggtcatcg gacaagagcg cgatttctgc tgtttcaatg gcaacgtccg
     2461 tgccacccaa ccccatcgct actcccacgt tcgcggtcgc cagagccggg ccatcgttca
     2521 caccatcacc cacaaaggca acattgagtc cctgggcctg gagttgcttg acgatctcca
     2581 ccttctgcgc tggaagtact tcagcatgta tgtcgctgat ccccaactcc ccagcgatgc
     2641 gctcagccgt gacgacgtta tcaccggtca cgaggatggt gcgaatgcct tgcttcttca
     2701 gtcgtgtaat cacctggctc gcctctggcc gtaacgtatc ttccagggcg agcataccaa
     2761 taacctgacc gttgcgtgcg accaggatga tagagcgtcc gtcagaggcc agattcttca
     2821 cgcgggcagc tatgcctgga tcgatggtga taccctgctc gaagagaagg cgtggtcggc
     2881 caataaccac ctctccacct tctatgcgta ctcgtacacc cagtcctggc agcgccgtaa
     2941 actcctctgg atcaggaaca gtcacctcgc gcgcgatggc cgcctgcacc actgcgcgtc
     3001 ccagaggatg ctcgcttagc
//
LOCUS       DASZAK010000382         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_258636, whole genome shotgun sequence.
ACCESSION   DASZAK010000382 DASZAK010000000
VERSION     DASZAK010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258636"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1566
                     /locus_tag="VGT44_06705"
     CDS             52..1566
                     /locus_tag="VGT44_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEV2580527.1"
                     /translation="MSETLVQTQYGKMQGIQEGAVYSWKGIPYAKPPLGELRFRPPQP
                     LEAWSDIRQATQFGPVAMQSPAIEKLAGRRLAMSEDCLTLNIWSPEADEKLRPVLVWI
                     HGGGFEIGSGSFHDGTPLAAMGDVVVVTFNYRLGAFGFLHLAEIGGEAFAGSGNCGLL
                     DQVAALEWVHNNIAVFGGDPQRVTVFGESAGAMSIAVLLALPTAHGLFHRAILQSGAA
                     QTVRSSSDATDLAGIFLKILDVSPQTLSTLKQIQAERLVEAASGLPDWRGTTGAGDTI
                     HFTPVIDGVILPQTPLQTLANGAAINISLVIGTTKDECLFYPFADPTWREADEEVLIQ
                     RCKHLVGAAWSYVSPFYLHGQPAGLPLLKRLLSLLTFDHFIFPAIQLAETLVNQGARV
                     WFYRFDWHSPFFDGAAHTLDRLFVWNLVASSAPVVERMTGNAPERLQLAHQMQRSWIA
                     FAHHGDPNTPELPLWPSYDLKARATMLFNRESLIQDDPDAEARQVWANASSLGR"
     gene            complement(1620..1907)
                     /locus_tag="VGT44_06710"
     CDS             complement(1620..1907)
                     /locus_tag="VGT44_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HEV2580528.1"
                     /translation="MSDTAAISPREHSVLNDQKIVMYVTTWCGDCRMAKRWFDSHNIP
                     YEMVNIEEDDDAAAYVAKVNRGMRSVPTILFPDGSVLVEPSPRELAAKFLE"
     gene            complement(1954..3216)
                     /locus_tag="VGT44_06715"
     CDS             complement(1954..3216)
                     /locus_tag="VGT44_06715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2580529.1"
                     /translation="MPEYIDVLIVGGGPAGLAAAEAAASKGVSTLALERQNEIGYPVH
                     TSGGSWIQDMKALGIPEHLYHPITSVFFVTPNREVALRYNPAVACVIDVRGVYQHLAG
                     RAVAAGAVLRVRHTAEQTLIEDGRVAGVTARNHQGERIELLAAVTIDASGFSRHIGVR
                     TEMGQAFHRYGYGAEYDLYAPQYPQDELYLVMGSRFAPRGYAWAFPRGNGRVRLGVGV
                     VHPDCDDDARLYLDRVMRELPQFAGKFKDASPIEYHTGLFPSEGPVASFSRDGLLLAG
                     DAAGHGSTLVGEGIRFAIYAGQMAGAVAAEAVKREDTSATYLKRFDRQWRARFGRDMD
                     IAYMINKRITAYSDEQWDGALDLMKRLTPAQMAQALRGDFSAGLVMGILARNPGLVAT
                     GGKKFLDLMLERINAGNIAHPHIVAGNE"
BASE COUNT          699 a          946 c          914 g          714 t
ORIGIN      
        1 acgaatgcgc ctgaaccgga attgcacgga gagtcataag gagatcattt catgtctgag
       61 acgcttgttc aaacacagta tgggaaaatg cagggaatcc aggagggtgc ggtctacagc
      121 tggaaaggca ttccctatgc aaaaccgccg ctgggggagc tgcgcttccg cccgcctcag
      181 ccgctggaag catggagcga tattcgccag gcgacgcaat ttgggccagt agctatgcag
      241 tcgccagcga tagaaaagct tgctggccgt cgcctggcaa tgagcgaaga ttgtctcact
      301 cttaacatct ggtctccgga agctgatgag aagcttcgcc cggtgctggt ctggattcat
      361 ggcggcggct ttgaaattgg ctctggctct tttcatgatg gaacgccact tgccgcaatg
      421 ggagatgtcg tagtggtcac gttcaactat cgcctgggag cgttcggctt cctgcatctt
      481 gctgagattg gtggcgaggc atttgctggc tctggcaact gcggcctcct cgatcaggta
      541 gcggctttag aatgggttca taacaatatc gctgtctttg gtggcgaccc gcaacgtgtg
      601 accgtttttg gcgaatcggc gggggcaatg agcatcgcgg tgctgctggc gcttcctact
      661 gcccatggtc ttttccatcg ggctattctg caaagcggag ctgcacagac agtcagaagc
      721 agctccgatg ccactgatct tgcaggcatc ttcctcaaaa tcctggatgt aagtccgcag
      781 accctttcca ccttaaagca gattcaggca gagcgattgg tcgaggcagc ctctggtctt
      841 cccgattggc gaggaacgac cggggctgga gatacaattc attttacgcc agtaatcgat
      901 ggtgtcattc tgccccaaac tcctctgcaa actttggcca acggcgcggc gataaatatt
      961 tcgcttgtga tcgggacaac aaaggatgag tgcctcttct accctttcgc cgatccgacc
     1021 tggcgcgagg ccgacgaaga ggtactcatc caacgttgta agcacctggt tggggctgca
     1081 tggtcctacg tttccccctt ctacctacat gggcaaccag ccggattgcc actcttaaag
     1141 cgacttctct cactgctgac ttttgaccat ttcatcttcc cggcgatcca gctcgcagag
     1201 acgctggtaa accagggagc gcgcgtctgg ttctaccgct ttgattggca ctcaccattt
     1261 tttgacgggg cggctcacac cctggacagg ctctttgtct ggaatcttgt cgcaagttct
     1321 gcgcctgtgg tagaacgcat gaccgggaat gcacccgaac ggctgcaact ggcccatcag
     1381 atgcaacgaa gctggatcgc atttgcacat catggagatc ccaatacgcc agaactcccc
     1441 ctctggccga gctatgacct gaaggcgaga gcgaccatgc tctttaaccg ggaaagcctc
     1501 atccaggatg atccagatgc cgaggcgcgc caggtctggg caaatgcttc ctccctggga
     1561 agataaaaag cacataaaaa agagcgacca tgatagtcgc tcttttcatt tgcgtcgtgc
     1621 tactcaagga acttcgctgc cagttcgcgt ggcgaaggct cgaccagcac cgaaccgtcc
     1681 ggaaagagaa tcgtcgggac gctgcgcatc cctcgattga cctttgcgac ataggcggca
     1741 gcatcgtcgt cctcttcgat gttgaccatt tcgtagggga tattatgcga atcgaaccaa
     1801 cgtttcgcca tgcggcaatc gccgcaccag gtagtgacat acatgacaat tttttgatcg
     1861 ttcaagacag aatgttcgcg aggagaaatc gcggcagtat cggacatcaa ataactcctt
     1921 gatgtgattt gaaagcagga taaacccgta aaattactca ttaccagcaa caatatgcgg
     1981 gtgagcaata ttcccggcgt tgatgcgctc caacatcaga tcaaggaatt tttttccgcc
     2041 agtggcgact agtccagggt tgcgtgccag gatgcccatg actagcccgg cgctaaagtc
     2101 gccacgcagt gcctgggcca tttgcgccgg ggtgaggcgt ttcatcaggt cgagcgcgcc
     2161 gtcccactgt tcatcggagt aggcggtgat gcgtttgtta atcatgtagg caatgtccat
     2221 gtcgcgacca aaacgcgccc gccactgccg gtcaaagcgt ttcaaataag ttgccgacgt
     2281 atcctcacgt ttgaccgctt ctgctgcaac cgcgcctgcc atctgacccg cgtaaatggc
     2341 gaaacggatg ccctcgccaa ccagggtaga gccgtgaccg gcggcgtctc ctgccagcag
     2401 caggccatcg cgcgagaagg atgccaccgg accctcggat ggaaacaggc cggtatggta
     2461 ctcgattggg ctggcatcct tgaatttccc ggcgaactgc ggcagctcgc gcatgacgcg
     2521 gtccaggtaa agtcgcgcgt cgtcgtcgca gtcggggtgg accacgccca cacccagtcg
     2581 cacacgcccg ttgccgcgcg ggaaggccca ggcataaccg cgcggcgcga agcggctgcc
     2641 catcaccagg tacaactcgt cctgcggata ttgcggcgcg tagaggtcgt attccgcgcc
     2701 gtagccatag cggtgaaacg cctggcccat ctccgtgcgc actccgatat gccgtgaaaa
     2761 accgcttgcg tcgatggtca cagccgcgag aagctcgatg cgctcgccct gatgattcct
     2821 ggctgtgacg cctgctaccc ggccatcttc tatcaacgtc tgttctgctg tgtggcgcac
     2881 gcgcagcacc gcgccagccg cgacggcgcg ccccgcgaga tgctggtaga cgccgcgcac
     2941 gtcgatcacg catgcgacgg caggattgta gcgcaaggcc acctcacgat tgggcgtgac
     3001 gaaaaagacg ctcgtaattg gatgataaag atgttcagga atgccaagcg ccttcatgtc
     3061 ctgaatccag cttccgccgc tggtatgcac ggggtagcca atctcgttct gccgctccag
     3121 cgccagagtg ctgactcctt tgctcgccgc ggcctcggca gccgccaacc ctgcggggcc
     3181 gccacccacg atcaatacat ctatatattc tggcatgaaa ttcctttatt actcacatgg
     3241 gttcgctggg atgcgatgaa gcatgtttcc aac
//
LOCUS       DASZAK010000383         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_684536, whole genome shotgun sequence.
ACCESSION   DASZAK010000383 DASZAK010000000
VERSION     DASZAK010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684536"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1702)
                     /locus_tag="VGT44_06720"
     CDS             complement(<1..1702)
                     /locus_tag="VGT44_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580530.1"
                     /translation="MNEYTPIDLIAFHNAGLLLLGEHGTAPIGPQQFRGLPFLVGANP
                     EHCFIAFGEGLQRAPLNIPINESAWSIIVAHRLLASGISAGGPVGELIAEYVFTYQSG
                     DEMRVPIRDRFEITEVPTAWGQLPFRAVPDQSDSLPERYKGRFDGSGGRQAEVNQAEP
                     RGYYLWAWQNPQPEQLRAALTVIPRGPRFLIAGVTLGHSDEYPFVRSGARPLKLTITD
                     TTVAAQPFDLAVAVDRGVTTFPYTLPRQSPDEFLVDPLAGWGEALNEQSSPASVEIAA
                     LPSATVTVMQGEDMLGTARWGEIEEKKIVELPRVRLELLDTGRNWVRTTVVDDETGQP
                     VPCRVHFRSPDGIPYQPHGHHSRVNSNMDTWHIDVGGDVRLGNISYAYIDGTCQGWLP
                     RGEVLVDVARGFEYEPLRTRVTVDPGQQDLTLRLKRWCNMNARRWFSGDTHVHFLSTQ
                     GSHTEAQGEDLNVVNLLQSQWGHLFTNTEEFTGGPSVSRDGRTIVWASQENRQHMLGH
                     LTLLGLKKAVMPWCTDGPSEAEMGGTLEVTMSDWADRCHTQGGTVILPHLPNPNGEPA
                     A"
     gene            2076..2522
                     /locus_tag="VGT44_06725"
     CDS             2076..2522
                     /locus_tag="VGT44_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV2580531.1"
                     /translation="MDDIDRSITRLLLANGRVSQEYLAREVHLSRPAVHERIKHLEEA
                     GVLRGYKALVDWDALGLPMASFIWVRTSGTACRVAGQTFMQLHSATAIIEECHRVTGE
                     WCMLLKVRVASPLALEQLLERIRDVTGVQDTMTILVLSTIAEDGRS"
     gene            2629..>3878
                     /locus_tag="VGT44_06730"
     CDS             2629..>3878
                     /locus_tag="VGT44_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV2580532.1"
                     /translation="MQDALGFLMDMRERYGDVVSVPTLFGPYSVAYHPDDVRHILQEN
                     HFNYNKDVIDYHVLRLVLGNGLLTNDGESWLRQRRLIQPAFHRERVAAFGTLMTETTL
                     AWLARWETGDFTETSKPLDLTQEMAGLALSIVGKALFSIDLSAETDRVGQALTTVNHL
                     LAEAFYLPWALSLPTPQRHRLHIARSVLHTVVEEIIRARRMRQEEHSDLLAMLLEARD
                     EETGEGMTDQQVRDEVLTLLLAGHETTANALSWTFFLLAQHPHVEAKLQVEYQHVLHD
                     RPPMIADLPKLPYNRMVIEEAMRLYPPAWGLGRRALGEDEIGSYSIPKGAYVLVFPYV
                     THRHPDFWERPDEFDPERFSPERVAGRPRFAYFPFGGGPRLCIGNQFALYEAQVILAT
                     ILSRYHLQLVPGASVTPEPLVTLRP"
BASE COUNT          812 a         1163 c         1076 g          827 t
ORIGIN      
        1 tggccgcggg ctcaccgttc gggttaggca ggtgcggcag aatcaccgtc cctccctggg
       61 tgtgacagcg gtcggcccag tccgacatcg tgacctccag tgtcccaccc atctcggcct
      121 cactcggacc atctgtacac cagggcatga cggccttctt caggccgagc aaagtcaggt
      181 gcccgagcat atgctggcgg ttctcctggg aggcccagac gatcgtgcgg ccatcgcgtg
      241 agacactggg accgccggtg aactcctccg tgttcgtaaa caagtggccc cactgggact
      301 gcaagagatt gacgacattg agatcctcgc cctgggcctc ggtgtggctc ccctgcgtag
      361 agagaaagtg gacatgcgtg tcgccgctga accagcgtcg agcgttcatg ttgcaccagc
      421 gcttgagtcg cagcgtcaaa tcctgctggc ccggatcgac ggtgacccgc gtgcgtaacg
      481 gctcgtattc aaagccacgg gccacgtcga ccaacacctc gccacgcggc agccagccct
      541 ggcatgtgcc atcgatgtag gcgtagctga tgttaccgag ccggacgtcg ccaccgacgt
      601 cgatatgcca ggtgtccata ttcgaattga cccggctatg gtggccatgc ggttggtagg
      661 gaataccgtc aggggaacga aaatggaccc ggcagggtac gggctgtcca gtctcatcgt
      721 cgactaccgt cgtgcggacc cagttccgtc cggtatccag tagttcaagt cgcacacgtg
      781 gcagctctac gattttcttc tcctcgatct ccccccagcg tgccgtcccg agcatatcct
      841 cgccctgcat caccgtgact gtcgccgacg ggagcgccgc gatctcgacc gaggcggggc
      901 tactctgctc attgagcgcc tcgccccaac cagccagggg atcgaccagg aactcgtcgg
      961 gcgactgccg gggcagcgta tagggaaacg tggtcacgcc gcggtcgacc gcaacagcga
     1021 gatcaaatgg ctgtgcagcc actgtagtgt cggtgattgt caacttgagg ggtcgcgcgc
     1081 cagaacgcac aaatggatat tcgtctgagt ggccaagggt tacaccagcg ataaggaaac
     1141 gtggcccgcg aggaatcaca gtgagcgcgg cccgcagctg ctcgggttgt ggattctgcc
     1201 atgcccacaa atagtagccg cgcggctcag cctgattcac ctccgcctgg cgccctccgc
     1261 tcccgtcaaa gcgtcctttg tagcgctcag gcaggctgtc gctctgatcg gggacggcgc
     1321 ggaaagggag ttgtccccag gcggtcggta cctctgtgat ttcgaagcgg tcgcggatcg
     1381 gcacgcgcat ctcatccccg ctctgatatg tgaagacata ctcagcaatg agctcaccaa
     1441 cagggccacc agcggagatg cctgacgcca gcaagcgatg ggccacgatg atcgaccacg
     1501 cactctcatt gatggggatg ttcaggggag cgcgctgcag cccttctcca aacgctataa
     1561 aacaatgttc cgggttggcg cccacgagga aaggcaagcc gcgaaactgc tgcggcccaa
     1621 taggggccgt cccatgctct ccaaggagca acaacccggc gttatggaac gctatcaggt
     1681 ctatcggagt gtactcgttc atcagtttcc tccttgggtg tgttcaatcg aacatacctg
     1741 ctgaacttta gaaccagagt tggggaggga aacgattccg cgcagcatca gccacgtctt
     1801 cacataagcc ctctctgcgg taccacctgc tctgagtgag attgctatgc tatttatcat
     1861 tctagcacaa attcgggata gacgccctcg catcttctac cattcagagc atcgacgaaa
     1921 tgtcggtctc ccttgcccac tcctacccca cagcgcactc tcaagtgacg attcgtcgga
     1981 attttttgta aacatagaca tttcgctttt tcttttgtat gtttataaac agagtgtcgt
     2041 actatcaaga accgcgcaac aagaggagga tcaatatgga tgatattgat agatcgatca
     2101 cacgactatt acttgccaat ggacgtgtca gccaggagta cctggcccga gaagtgcatc
     2161 tctcgcgacc agccgtccat gaacgaataa aacacctgga agaggcaggg gtgcttcgtg
     2221 gatataaagc cctggttgat tgggatgcac tcggccttcc tatggcatct ttcatctggg
     2281 tgcgcacgag tgggacagcc tgtcgtgtag caggccagac atttatgcaa ttgcactctg
     2341 cgaccgctat tattgaggag tgtcatcgcg tcacgggaga atggtgcatg cttctcaaag
     2401 tacgcgtcgc ttctcctctc gctttggagc agttgcttga acgtatccgt gatgtaacag
     2461 gcgtccagga taccatgacg atcctcgtgc tctcaacgat tgctgaagat gggaggtcgt
     2521 gacttctctt gtaagtcttc taaaccccaa actttccaac gaaagcacag cggacaccac
     2581 ccgcctggac cgccatggta tacagcgggc cataatacaa tccgcctgtt gcaggacgct
     2641 cttggcttcc tcatggacat gcgcgagcgc tatggggatg tcgtctctgt tccaacactc
     2701 ttcggccctt actctgttgc ctatcatccc gacgatgtcc ggcacatcct acaggagaat
     2761 catttcaact acaacaagga tgtgattgac tatcatgtct tacgcctcgt gcttgggaat
     2821 ggcctgctga ccaacgatgg cgaatcgtgg ctccggcaac gccgcttgat ccaacctgcc
     2881 ttccatcgag agcgcgtcgc cgcattcgga acgctcatga cagaaacgac gctcgcgtgg
     2941 cttgcgcgtt gggaaaccgg cgatttcact gagaccagca agccgctaga tctcacacaa
     3001 gaaatggcag gcctggcctt gagtatcgtg ggcaaagcgc tcttcagtat tgacctgagc
     3061 gcggagacag atcgtgtcgg gcaggcgctc accacggtga atcatctgct ggcagaggca
     3121 ttctacctgc catgggcgtt atcgctgcca acgccgcaac gtcaccgcct gcatattgcc
     3181 cggagcgttc tacatacggt ggttgaggag attattcgtg cgcgtcgtat gcgtcaagaa
     3241 gagcatagcg atctcctcgc tatgcttctc gaggcacgtg acgaggagac cggagaaggt
     3301 atgaccgatc aacaagtgcg cgacgaagtg ctcacgttgc tactcgccgg tcacgaaacg
     3361 acggccaacg cgctttcgtg gaccttcttc ctgctcgcgc agcatcctca cgtcgaggcg
     3421 aaactccagg tggaatacca acacgttttg catgatcgtc ccccgatgat cgcggatctc
     3481 ccaaaactcc cttataaccg catggtgatt gaggaagcca tgcgcctcta tccaccggcc
     3541 tggggcctgg gccgtcgcgc tctgggcgaa gacgagattg ggagctactc cattcccaaa
     3601 ggagcctacg ttctggtctt cccttacgtg acgcatcgcc acccagactt ctgggagcgc
     3661 cctgacgagt tcgatcctga acgattctct cctgagcgag tggcaggacg cccacgcttt
     3721 gcctacttcc cttttggggg aggcccacgc ctgtgtattg gcaaccaatt tgcgctctat
     3781 gaagcacagg taatcctggc gaccatcctg tcgcgctatc acctgcaact cgtgccaggt
     3841 gcatccgtca cccccgagcc gctggtgacg ctgcgccc
//
LOCUS       DASZAK010000384         6131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_185983, whole genome shotgun sequence.
ACCESSION   DASZAK010000384 DASZAK010000000
VERSION     DASZAK010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6131
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185983"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="VGT44_06735"
     CDS             complement(<1..1252)
                     /locus_tag="VGT44_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV2580533.1"
                     /translation="MQVLDAMTQAEQTKQLVKREDIRNVAIIAHVDHGKTTLVDGLLK
                     QSHIFRENQQVGELIMDSNDQERERGITILAKNTAIIYKGVKINIIDTPGHADFGGEV
                     ERVLNMADGCILLIDAVEGPMPQTRFVLQKALELNLKPIVIINKIDRRDARIEQVLEW
                     TQDLFLELATSDTHLDFEVLYAVGRDGVAMYQPGDERVNLEPLFETIINTIPAPVVDV
                     DAPLQLLVAALDYDDYKGKYAIGRIVRGRVTPNSFVAHINRDGVVTRQKINLVLTYKG
                     LQRVEVAEAHAGDIVALTGIASANIGETIADLETPEALPTIAISEPTLKMTFGVNSSP
                     FAGRESKYPTSRQRRARLYRELETNVSLRVQDGSSPDEFVVSGRGELHLSVLIETMRR
                     EGYEFQVSRPEVITREDGGQLVEPM"
     gene            1439..2587
                     /locus_tag="VGT44_06740"
     CDS             1439..2587
                     /locus_tag="VGT44_06740"
                     /inference="COORDINATES: protein motif:HMM:NF020318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1786 family protein"
                     /protein_id="HEV2580534.1"
                     /translation="MPETIRILTVDVGTGTQDILLFESGKTIENCFKMVMPSPTMIVA
                     ERIRRATERGQAVLLTGVTMGGGPSHWAARDHALAGYAVAVTPEAGRTFDDDLTQVEG
                     MGFELIDEDEAARRIEGNSWLHVELQDFDAQAIITALRAFDVSPAVDALAIAAFDHGA
                     APPGYSDRRFRFDFLARTVRQQPVPSAFAYPAQQTPVDLTRLRAVAQSAARYVRQSDS
                     RPDTPLLLMDTGSAAVLGTLEDPLVRAQRESILCNVGNFHTLAFHLINGKIVGIFEHH
                     TGEIDRAHLEGMLIKLANGTLTNEEVFDSSGHGALILNGETAQGHRFPFLAVTGPRRE
                     LLRGSALQPYEATPHGDMMLAGCFGLLRAFADHYPAMAGAIEGSLLRA"
     gene            complement(2609..3049)
                     /locus_tag="VGT44_06745"
     CDS             complement(2609..3049)
                     /locus_tag="VGT44_06745"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2580535.1"
                     /translation="MALRSDELHYNLQHLNGMLTTHEAARQLDLSYWHFMHLVEAGRI
                     PGVRVVDRWLFSPEDLDEYRRSKYGELEDMARTALEHPAVDLTEKQETICRYLASSER
                     PSEIARKLQQSRQAVHSQIVLIKEKVSRIQHKRPIPLRPPKKKS"
     gene            complement(3168..5975)
                     /locus_tag="VGT44_06750"
     CDS             complement(3168..5975)
                     /locus_tag="VGT44_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2580536.1"
                     /translation="MVDRIGQQLGNYRLTQLLGQGGFADVYLAEHTFLSTQAAIKLLH
                     TRLTGDELQAFLNEARTIAHLIHPNIVRVLEYGVENGVPYLVMDYAPGGTLRQRHPRG
                     TALPLPLIVSYVRQVAAALQYAHNQKLIHRDVKPENMIIGRNNEVLLSDFGVALIAQS
                     SLSQSVHEVAGTVTYMAPEQIRGKPRFASDQYALAVVVYEWLCGERPFQGSFTEVAAQ
                     HALAPPPPLHERMPTISPYIEQVVMTALEKDPARRFASVQSFANAFEQACQTMPQPAA
                     NPPSQPGWNNWQGGIYQPGVTNPTPVIEVAQNAQTLASAPPPVPQHPSAFYTAIATPA
                     GGTPPTMTPKDWSGSKIGDSARKRSPLLASVLVILVVMVVLAGSGALYYVIRSHTTGG
                     GATPTAVPVAAGGTWSDDLFQQPDSLIPNAGIDTSAGIMDQALWAPLFYADSQGVIQP
                     GLAMVVPSLANNGVSSDLKTWTFHLRPNLKWSDGQPLNADDVNFTWQLWANQQFVAAS
                     TVGINMITSADVSADKLTITFHLSQPFAPFLAAWVDGLNAPMPAHHYSGKTPGAILSS
                     SDAQSPPVTSGPFEISQTQAGLDYNLLRNPNYYLASQGMPHLDGIKFHVVKDQAATLQ
                     DLQAGTIDSAWFLDVSKTIPYQQLSNYVLERSAASANFEALYFNFHNPILGKYPEVRR
                     AIAMAIDQQTLIDAARRGEAAPLCTDHGSAYHPGYQANAPCPKFDPNAATTLLDQNGW
                     TTGSDGVRAKNGMRLSFEYSSAYSADTNSLWRADDEFIVQANLKAIGIQLVIQNYPAS
                     TFFGTFLPGGKSSPATGAQAGMYDIAEFQNVFYFDPDDSAYFSCNQFPPTGANYDFYC
                     NPALDQLYTKERTTLDPTQRQQIFNQIHQIYLTDFPFIVLYSPLDISLHKKTVHNYAP
                     GAFGAVEGTNIWEWWCANGQC"
BASE COUNT         1201 a         1687 c         1954 g         1289 t
ORIGIN      
        1 ccatcggctc taccagctgg ccgccatctt cgcgcgtgat gacctccggg cgcgagacct
       61 ggaattcgta gccctcgcgg cgcatggtct cgatcagcac agagaggtgc agttcgccgc
      121 gcccggagac gacgaattcg tcgggcgagc tgccatcttg gacgcgcagg ctgacgttgg
      181 tctccagctc gcgatagagg cgcgcgcgcc gttgccgcga ggtgggatac ttgctctcgc
      241 gcccggcaaa ggggctggaa ttgacgccaa aggtcatttt cagcgtcggc tcgctgatgg
      301 cgatggtcgg cagtgcttcg ggcgtctcca ggtcggcaat cgtttcgccg atgttcgcgc
      361 tggcaatgcc tgtcagcgcc acgatatcac ccgcgtgggc ctcggcgacc tcgacacgct
      421 gcagaccttt gtaggtcagc acgagattga tcttctggcg cgtgaccacg ccgtcgcggt
      481 tgatgtgcgc cacaaagctg ttgggcgtga cacggccacg cacgatgcgc ccgatggcat
      541 atttcccctt gtaatcatcg tagtccaggg cggccaccag cagttgcagg ggcgcatcga
      601 catccacgac gggcgcggga atggtgttga tgatcgtctc gaacaacggc tccaggttga
      661 cgcgctcgtc gcccggctgg tacatggcga cgccatcgcg cccgacggca tacagcacct
      721 cgaaatcgag atgcgtgtcg ctggtggcga gttccaggaa gaggtcctgc gtccattcga
      781 gtacctgctc gatgcgcgcg tcgcggcggt caatcttgtt gatgatcaca atcggcttga
      841 ggttgagttc cagcgccttt tgcagcacga agcgcgtctg cggcatgggg ccttccaccg
      901 cgtcgatcag caggatgcag ccgtcggcca tgttcagcac gcgctcgacc tcgccgccaa
      961 agtcggcatg gccgggcgtg tcgataatat tgattttgac gcctttatag atgatggcgg
     1021 tgtttttcgc caggatggtg atgccgcgct cgcgctcctg gtcgtttgaa tccataatca
     1081 gttcgcccac ctgctggttc tcgcgaaaga tatggctctg tttgagcagc ccatcaacga
     1141 gcgtcgtctt gccatgatcg acgtgggcaa taatggcgac attgcgaata tcttctcttt
     1201 ttaccagttg ttttgtctgt tctgcttgcg tcatagcgtc taatacctgc atagtaaccc
     1261 gattcactcc ccaaaggcat aagaaaaccg gacggctaaa gtcgcccggc gtttatgatt
     1321 gactccgtac cgatgattgc tggttacatt gtatcccctg gcggcgattc cgtcaagcgt
     1381 gttcacaagg tgatatactc caatataatg cttatttcag aggagtaatt gaagacccat
     1441 gcctgaaacc atacgtattt tgactgtgga cgttggcacc ggcacacagg atattttgct
     1501 gtttgagagc ggcaaaacca ttgagaactg tttcaagatg gtcatgccgt cgcctacaat
     1561 gattgtggcg gaacgcattc gacgcgcgac cgagcgaggg caggcggtgc tgctgacagg
     1621 cgtgacgatg gggggtgggc cgtcgcactg ggcggcgcgc gatcatgccc tggcgggcta
     1681 cgcggtcgct gtgacgccgg aggccgggcg cacctttgac gacgatctga cgcaggtaga
     1741 gggtatgggc tttgaactga tcgacgagga tgaggcggcg cggcgcattg agggtaatag
     1801 ctggctgcac gtcgaattgc aggatttcga tgcccaggcc atcatcaccg cgctgcgagc
     1861 attcgacgtg agtccagccg tggatgccct ggcgattgca gccttcgatc atggcgctgc
     1921 accccccggt tatagcgacc ggcgcttccg cttcgatttc ctggcgcgca ccgtgcgcca
     1981 gcagcctgtg ccaagcgcct tcgcctaccc cgcgcagcag acgcccgtcg acctgacgcg
     2041 cctgcgagcg gtggcgcaga gcgcggcgcg ttatgtgaga cagagcgatt cgcggccaga
     2101 tacgccgctg ctgctgatgg atactggctc agctgctgtg ctggggacac tggaagaccc
     2161 gctggtcagg gcgcagcgcg agagcattct atgcaacgtt ggcaactttc acacgctggc
     2221 cttccacctt atcaacggga aaattgtggg cattttcgag catcataccg gcgagatcga
     2281 ccgcgcgcac ctggaaggca tgctcatcaa gctggccaac ggcacgctga ccaacgagga
     2341 ggtctttgat tcgagcgggc atggcgcgtt gatcttgaac ggcgaaacgg cgcaaggcca
     2401 tcgcttcccc ttcctggcag tgacggggcc gcgccgcgag ctgctgcggg ggtcagcgct
     2461 ccagccatac gaggctacgc cgcacggaga tatgatgctg gcgggctgtt ttggcttgct
     2521 gcgcgccttc gcggaccatt acccggcgat ggctggcgcg atcgaggggt cgctcttgcg
     2581 ggcatagcga cgattgaata tcgcgtctct acgacttctt cttcgggggg cgcaggggga
     2641 tggggcgctt gtgctggatg cgcgagactt tttctttgat gaggacgatc tgagaatgaa
     2701 cggcctgccg tgattgctgc aacttgcgag caatttccga ggggcgttcg ctgctggcca
     2761 ggtaacggca aatggtttct tgcttctcag tcaggtctac ggcgggatgt tcgagggcgg
     2821 ttctcgccat atcttcaagt tcgccgtatt tgctgcggcg gtattcgtcg aggtcctcgg
     2881 gggagaaaag ccagcgatca acgacgcgca cgcctggaat gcggcccgct tccacgaggt
     2941 gcatgaagtg ccagtagctc aagtctaatt gcctggctgc ttcatgggtg gtaagcatac
     3001 cattcagatg ctgtagatta taatgaagtt catcgctcct gagagccatg atcggatgcc
     3061 tttctgcgtt tcaacggcca gccctgctcc aatcgccgga aaaagacatt cgttgtcatg
     3121 gtgagtatac catagagatt gagctatctg caaggattat agatagatca gcattgtccg
     3181 ttggcgcacc accattccca gatgtttgtt ccctccactg ctccaaacgc gcctggggcg
     3241 tagttgtgga cggtcttttt gtgcagcgag atatccagcg ggctgtagag gacgatgaag
     3301 gggaaatcgg tcaggtaaat ctggtggatc tggttgaaga tctgctgccg ttgggttggg
     3361 tcgagcgtgg tgcgttcctt tgtatagagc tgatccaggg cggggttgca ataaaaatca
     3421 tagttggccc cggtgggtgg gaactggttg caggagaagt acgcggaatc gtcgggatca
     3481 aagtagaaaa cgttttggaa ctcggcgata tcgtacatgc ccgcctgagc gcctgtggcg
     3541 ggagaggatt tgccgcctgg gaggaaggtc ccgaaaaatg tggaggcggg atagttctgg
     3601 ataacgagct gaatgccgat ggccttcagg ttcgcctgca cgataaattc gtcatcggcc
     3661 cgccacaggc tgttggtatc ggcagaatag gccgacgaat actcgaacga caggcgcatc
     3721 ccattctttg cgcgcactcc gtcgctgccc gtcgtccagc cgttctgatc cagcagggta
     3781 gtggccgcgt tgggatcgaa tttggggcag ggcgcgttcg cctggtagcc gggatgatag
     3841 gccgagccat ggtcggtaca caggggcgca gcttccccac gccgcgccgc gtcgatcagg
     3901 gtctgctggt ctatggccat cgcgatggcc cggcgcacct cgggatattt gcccaggatg
     3961 ggattgtgga agttgaagta gagcgcctcg aaattcgcgc tggcggcaga ccgttcgagg
     4021 acatagttcg agagctgctg gtatggtatc gtcttgctca cgtcgagaaa ccaggcggag
     4081 tcgattgtgc cagcctgcaa gtcctggagc gtggcggcct ggtccttgac gacatgaaac
     4141 ttgatgccgt ccaggtgggg catcccctgc gacgccaggt agtagttcgg attgcgcaac
     4201 aggttatagt caagtcccgc ctgggtctgg ctgatctcga aggggccgga cgtaacgggc
     4261 ggggactggg catcagacga gctgagaatg gctcccggtg tcttcccgct atagtggtgc
     4321 gccggcatgg gagcgttcag gccatcgacc caggcggcca ggaaaggcgc gaagggctgg
     4381 ctgaggtgaa aggtaatcgt cagcttatcg gcggagacat ctgccgaagt aatcatgtta
     4441 atgccaacgg tactcgcggc aacgaactgc tgattagccc agagctgcca ggtgaaattt
     4501 acgtcatcgg cgttaagagg ctggccatcc gaccacttca ggtttgggcg caggtggaat
     4561 gtccaggtct tcaaatcgct gctgacgccg ttgttggcaa gactcggtac aaccatagcc
     4621 aggccgggtt gaatgacgcc ctgcgagtca gcataaaaga gcggcgccca gagcgcctgg
     4681 tccataatac ccgcggaagt gtcgatcccg gcattgggga tgagcgagtc gggctgctgg
     4741 aacaggtcgt cgctccaggt gccgccagcc gcgacgggaa ccgcggtggg cgtcgcgcct
     4801 ccaccggtgg tatgcgatct gataacgtaa tacaaagccc cgctccccgc caggaccacc
     4861 atcaccacca gaatcacaag cacactcgcc agcagcggag agcgtttgcg agcgctatca
     4921 ccgattttcg atccgctcca gtccttaggc gtcatggtgg ggggcgtccc gccagccggt
     4981 gtcgcgatgg cggtatagaa cgccgaaggg tgctgcggga cgggaggagg agccgaggca
     5041 agcgtttgag cattttgcgc cacttcaatg acgggagtgg gattcgttac gccgggctga
     5101 tagatgcctc cctgccaatt attccagccg ggctgggatg gaggattcgc ggcgggctga
     5161 ggcatcgtct gacatgcctg ctcgaaggcg ttggcaaagg actgcacgga ggcgaaacgc
     5221 cgggcggggt ctttctcgag cgcagtcatg acgacctgtt cgatgtaagg cgagatggtt
     5281 ggcatcctct cgtgcaaagg cgggggagga gcgagcgcgt gctgggccgc aacctcggtg
     5341 aaggagcctt gaaaaggtcg ttctccgcaa agccattcgt agaccactac cgccagggca
     5401 tactggtcgc tggcgaaacg cggcttgccc cgaatctgtt cgggcgccat gtaggtgacg
     5461 gtgcctgcca cttcgtggac gctctgcgac agggaactct gcgcgatgag ggccacgccg
     5521 aaatcgctga gcagcacttc attgttacgc ccgataatca tgttctccgg cttcacatcg
     5581 cggtgaatga gcttctggtt atgggcatac tgcaaagcag cggcgacctg cctgacgtag
     5641 gagacaatta agggcagtgg aagagcagta cccctggggt ggcgttggcg caacgtgcca
     5701 cctggggcat agtccatgac taaataagga acgccgtttt cgacgccgta ctccagaacg
     5761 cgcacgatgt tggggtggat caggtgagca atcgtgcgcg cctcgttgag aaatgcctgc
     5821 aattcatcgc ccgtcaggcg cgtatgcagc agcttgatgg cggcctgcgt ggaaagaaag
     5881 gtgtgttccg ccaggtagac atccgcaaag cccccctgcc ccagcaactg ggtcaggcga
     5941 tagttgccga gctgctgtcc tattcggtcg accatctgcg ttcctcctca aaaaatgccc
     6001 tttcaccaac cctccctatc cagaaagcat agatacctat acatgaagct ggacgatatt
     6061 ttacaccaga catgtgacat cggcaagcca tgcgacagag gacgagggcg accgcaaggg
     6121 tctccacccc c
//
LOCUS       DASZAK010000385         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_470957, whole genome shotgun sequence.
ACCESSION   DASZAK010000385 DASZAK010000000
VERSION     DASZAK010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470957"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1760)
                     /locus_tag="VGT44_06755"
     CDS             complement(<1..1760)
                     /locus_tag="VGT44_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2580537.1"
                     /translation="MSNEQQGQHKQEGQQAQFRGSYEVIVVGAGHAGCEAALACARMG
                     RRTLLVSMNLDSVALMPCNPSMGGPAKGHLIKEIDALGGEIGRNTDRTFIQVRRLNTS
                     KGPAVQALRAQCDKQAYRLAMKFVLENQPNLELKQATILCLLSEPGADGRPAIRGVVA
                     SNGWQYEAQAVILTTGTFINGRLVVGEKTQPGGRAGEGPALGISDSLRALGLEVRRFK
                     TGTPPRIDARTIDFSKTDPQPGSRVPLYFSQDRSAREDVQLPGIRPNPVYPVTDEDLY
                     GWRPQLPCYLVHTNEATHQVIRDNLHRSPLYTGVIEGIGPRYCPSIEDKIVRFADKDR
                     HQIFLEPEGWRTGEVYVQGTNTSLPEDVQLAMLRTIPALKHAEIMRFGYAVEYDFVPP
                     DQLLTTMQTRPVAGLFLAGQINGTSGYEEAAAQGIMAGINAALYVRGEAGFVLGRHEA
                     YLGVLVDDLVTRPLTEPYRLHTSRAEYRLLLRPDSADLRLSDYGYRFGLIDDRRYEQV
                     ARKREEIAHAMQQIDRVVFTSSRSTESRAQELGIEPLGGKLTARELLRRPDVTYTQVA
                     RLSQSQPQHSAQTSTGDQTV"
BASE COUNT          346 a          610 c          645 g          399 t
ORIGIN      
        1 aaaacggttt gatcgccggt tgaggtctga gcgctgtgct gaggttgtga ctgtgacagg
       61 cgagccacct gagtataggt cacgtccggt cggcgcagta gttcgcgggc ggtcaacttg
      121 ccgcccagcg gctcgatgcc gagttcctgc gcacggcttt cggtgctgcg cgacgaggtg
      181 aacacaacgc ggtcgatctg ctgcatggcg tgggcgatct cctcgcgttt gcgcgcgacc
      241 tgctcgtagc gccggtcgtc gatcaggccg aagcggtagc catagtcgct caggcgcaaa
      301 tccgcgctgt cgggccgcag cagcaggcga tattcggcgc gcgaggtatg caggcggtac
      361 ggctcggtca agggccgtgt cacgaggtca tctaccagta caccgagata tgcttcgtgg
      421 cgacccagca cgaaaccggc ctcgccgcgc acataaaggg cggcgttgat gcccgccatg
      481 atgccctgcg cggcagcctc ttcgtagccg gaggtgccgt tgatctgccc cgccaggaag
      541 agacccgcga cgggcctggt ctgcatggtg gtcagcaatt ggtcgggcgg tacgaagtcg
      601 tattcaacgg cgtagccgaa gcgcatgatc tcggcgtgtt tcagggcggg gatggtgcgc
      661 agcatggcca gttgcacatc ttccggcagg ctcgtattcg ttccctgcac gtacacctcg
      721 cctgtgcgcc agccctccgg ttccaggaaa atctggtggc ggtccttgtc ggcgaagcgc
      781 acgatcttat cctcgatgga ggggcagtag cgcgggccga tgccttcgat gacgccagtg
      841 tagagtggcg agcggtgcag gttgtcgcgg atgacctggt gcgtggcctc attggtatgc
      901 accaggtaac agggcagttg cggacgccag ccatacaggt cttcgtccgt cacagggtag
      961 acggggttcg gtcgtatccc cggaagttgc acatcttcgc gcgccgaacg atcctgcgag
     1021 aagtagaggg gaacgcggct gccgggctgc gggtcggtct tgctgaaatc gatggtgcgc
     1081 gcgtcgatac gcgggggcgt tccggtcttg aaacgacgca cctccaggcc cagggcacgc
     1141 agcgagtcgg agatgccgag agcgggacct tcgccggcgc gcccgcccgg ctgcgtcttc
     1201 tcgcccacga ccagccgccc attgatgaag gtgccggtcg tcaggatcac cgcctgggct
     1261 tcgtactgcc aaccattgct ggctaccacg ccgcgtatcg ccgggcggcc atccgcgccc
     1321 ggctcgctga gcaggcacaa gatggtggct tgcttcaatt cgaggttggg ctggttctcc
     1381 agcacaaatt tcatggccag ccggtaagct tgcttatcgc actgcgcgcg cagcgcctgc
     1441 accgccgggc ctttgctggt attgagccgc cgcacctgga tgaaggtgcg gtcggtattg
     1501 cgcccgattt caccgcccag cgcgtcgatc tccttgatta aatgcccctt ggccgggccg
     1561 cccatggagg gattgcaagg catcagcgcc acgctgtcca ggttcatcga taccagcagc
     1621 gtccggcgtc ccatgcgcgc gcaggccagc gctgcctcgc agcccgcgtg tccggcgccg
     1681 acgacgatca cctcgtagct gccgcgaaac tgcgcttgct ggccttcttg cttgtgctgt
     1741 ccttgctgtt cgttgctcat cgttgctaaa ctgcctctca tccccacaaa tgacacctgt
     1801 actggtgcgt caactgcaat tgatcagtag gtgccgattt atcgcgcaca ccgccgataa
     1861 tgaacattaa caaattaagt tggagataag ccaacatagt ggagcagatc cgatgagggc
     1921 tgatcgtact gatcgagcca gtcctggacg cgctcttgca agtccttcca gcgactgcta
     1981 taacgatgca agtgcagcag
//
LOCUS       DASZAK010000386         1855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_365204, whole genome shotgun sequence.
ACCESSION   DASZAK010000386 DASZAK010000000
VERSION     DASZAK010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365204"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..1397)
                     /locus_tag="VGT44_06760"
     CDS             complement(423..1397)
                     /locus_tag="VGT44_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV2580538.1"
                     /translation="MMYALQISRFGEPSDVLELVKLPDPHDPLANEVLLAVEYAPISD
                     SDLSTIRGQYGMLPSLPTGIGFEGVGRILSVGEEVDHLKVGDRVLLPSSSSSWREKII
                     LPSTGLFPLPQNADPQQLSMLSINPPTAALLLSEYVELKPGDWVIQNAGNSGVGRSVI
                     AFAKDRGLRTVSLVRRPELIDELVAAGGDIVLVDGAEISARVAQATGNAKIALALDGV
                     GGEAMLSLSSCLSPGGTLVVYSAVSKKPSLASAMDLVFRIVTIRGFWLYSPEFRASPK
                     VVEAIKTGVRLIAEGKLYMPVAATYPLVAAKEAIAHAQRGGKVLFKLS"
     gene            complement(1409..>1855)
                     /locus_tag="VGT44_06765"
     CDS             complement(1409..>1855)
                     /locus_tag="VGT44_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2580539.1"
                     /translation="LVIAEELKGKVIDLEGRDLVVVEVGHTDTDDTTCLHVPSIGLVV
                     AGDAAYNDVHLYLAESHAQTRREWIAALDTIESLHPRAVIAGHKRPGSDDDPRIIEET
                     RQYIRDFDRLAETTTTARELYDQVLSLYPQRVNPGALWLSARALKP"
BASE COUNT          372 a          559 c          535 g          389 t
ORIGIN      
        1 acctccgcta cgagtggtct aaccgacctt gtatcatagc agaggcccct gtcaatatac
       61 gcctactctg caaagaagga cgtcaggtat tggacgactt cctgaggatg ttcttcaggc
      121 agccaatgcc cggagttggt gatgacgaca ctccttacgt gattcgcata ctgctgaact
      181 tgctgtccca cctttgctcc caggctgtgg tctgctccta tagcaagaac gggcatagac
      241 actttttcat ggcggagctg gaggatgttc ctgtgccagg cactcagcac gctccctaac
      301 gctagccgca acggccgtac ctgtgctcgc agaccatcag cgcgttgttc aattcttgcg
      361 acagattttc cacccgtctc cagcgctatg ggcgcattgt agatggcgag catctgaggc
      421 cattatgaga gtttgaacag gaccttcccg cccctttggg catgagcgat cgcttccttc
      481 gccgcgacga gaggataggt tgctgcgaca ggcatgtaga gcttcccctc ggcgatcagc
      541 cgcacacccg ttttgatggc ctccaccact ttaggggaag cgcgaaactc cggactgtag
      601 agccagaagc cgcgtatcgt cacaatgcga aagacgagat ccatagcgct tgccagactg
      661 ggttttttgc tcaccgcgga atagaccacc agcgtgcccc cagggctcag gcaactggac
      721 agagagagca tcgcttctcc cccgacgccg tcgagcgcta aggcgatctt cgcgttgccg
      781 gtggcttggg ccacccgagc ggatatctct gctccatcca ccagcacgat gtcacccccc
      841 gcagctacga gttcgtcgat gagttcgggc ctccgcacca ggctcacggt tcgcaaccca
      901 cgatcctttg caaaagcgat taccgagcgc ccaacgccag aattgccagc attttgaatc
      961 acccaatcgc cgggcttgag ctcgacgtat tcgctcagaa gcagtgcagc cgtcggtgga
     1021 ttgatgctca acattgacaa ctgttgcgga tcagcgtttt gtggcagcgg gaagagcccc
     1081 gttgacggca atatgatctt ctcgcgccag gaggaggagg aagatgggag aagcacacga
     1141 tcgccgacct tgagatggtc aacctcctct cccaccgaca ggattcgccc gacgccctca
     1201 aagccgatgc cagtgggcaa ggaaggcagc ataccgtact ggcctctgat cgtcgagagg
     1261 tcactatcgc tgatcggcgc gtattccacg gcgagcagca cctcgttggc aagaggatcg
     1321 tgcggatcgg ggagcttcac cagttccaat acgtcggatg gttcgccaaa cctcgaaatc
     1381 tgaagagcat acatcatctt tctccttcct atggcttgag tgcacgcgct gataaccaga
     1441 gtgcaccggg gttgacccgc tgcggataga gcgagagcac ctggtcgtag agctcgcgcg
     1501 cggtcgtcgt cgtctcggct agccggtcga agtcgcggat atactgccgg gtctcttcga
     1561 tgatccgggg gtcatcgtcg ctcccaggtc gcttatggcc ggcgatcacc gcgcgtgggt
     1621 ggagcgactc gatggtgtcg agcgccgcga tccactcgcg gcgggtttgc gcgtgtgact
     1681 cggcgaggta gaggtggacg tcgttgtagg cggcatcgcc tgctacgacc aggccaattg
     1741 acggaacatg caggcaggtc gtgtcgtcgg tgtcggtgtg cccgacttcc accacgacca
     1801 ggtcgcgccc ttccagatcg atcacctttc ccttgagttc ctcagcgatc acgag
//
LOCUS       DASZAK010000387         5997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_436301, whole genome shotgun sequence.
ACCESSION   DASZAK010000387 DASZAK010000000
VERSION     DASZAK010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5997
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436301"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..154)
                     /locus_tag="VGT44_06770"
     CDS             complement(<1..154)
                     /locus_tag="VGT44_06770"
                     /inference="COORDINATES: protein motif:HMM:NF025165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase-like
                     domain-containing protein"
                     /protein_id="HEV2580540.1"
                     /translation="MDDLSIFTGNANRPLAASICKHLRIPLGDADVFQFSNENIFVKI
                     NDNVRGR"
     gene            337..2796
                     /locus_tag="VGT44_06775"
     CDS             337..2796
                     /locus_tag="VGT44_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV2580541.1"
                     /translation="MLDQSNLLAGLNEPQQQAVTTTEGPVLILAGPGSGKTRVITHRI
                     AYLVQHNHVSPWRILAVTFTNKAAKEMRERLEKLVGINESKEMTIGTFHAICARVLRT
                     EADYLAPIGLNRSFVIFDTDDQAALVKQAIKGMDLDEKQYRPLTIQAMISRAKNDLQS
                     PDQMAELAAKYLEEVAARVYKQYQKLLRANNAVDFDDLLMLTEQLWRREPEVLRKYQA
                     RWHYIHVDEFQDCNLPQYKLIRLLGFGTDERHEGLGNVCVVGDDDQMIYTWRGASAEN
                     VLRFERDFPQTKIIMLEQNYRSTQTILDAAQNVVQRNRLRKDKRLWTALGTGEKIILH
                     EAFDEEEEGLFTAREIKRLQGRGDIDKLGDVAVMYRTNAQSRALEEQFLRANIPYKVI
                     GSRKFYERKEIKDMLAYLRLLANPYDDLSMQRIINVPNRKIGPKTMGELQRWAAERHI
                     ALYDAIQQIDEHPTLGKAAKLALSGFGQLLLDFTNAIKELHLPELLDRIAERSGYGPE
                     LRETPEGEERWSNVLELRRVAADYSEIETRTALELFLENVALVGGADTAQTGENGTLI
                     KEEQNDAVTLITLHAAKGLEYPVVFIVGMDEGSLPHARSVDKPEQLEEERRLAYVGFT
                     RAMRRLYLVRAYRRSFFGESQVTEPSRFLEDIPLHLVSNQGAASSHSPGGLRSSATRR
                     GSWGDDYNYNQDTHSSRETGRVFGSGKPQVNSQPPQRMARPSSYIAPKTPSTLPSQKG
                     NRDAASPPSSPTTPAAPANPAKPTSQQYKPGDRVRHDKFGEGIILKSEMEQNTEFVEV
                     QFQGKHGKKRLSMDFAKLEKL"
     gene            2928..3680
                     /locus_tag="VGT44_06780"
     CDS             2928..3680
                     /locus_tag="VGT44_06780"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEV2580542.1"
                     /translation="MASFHFPVRRAMVCLILTIASALLIAGCTPPWQHGSAAVANSLA
                     PKPTAQQLLNTLQKNFRSVTTFHVTLKVDNLGTTSGSQIQIRTADGDVIMPDKVKAQA
                     NVLFSGQPVTVKLISIGNIQYLTDPITGQWRVVKGVLNASTLTNPDTGLISLATKLQN
                     VTGPTDDAVHGTPCWRVNGMMDAKSLAFFTGGGVPAGTMLQASICVGKADGLPYQLSV
                     VGQAAKSDTLQTSRTFTISGYNETISITAPQF"
     gene            3801..>5997
                     /locus_tag="VGT44_06785"
     CDS             3801..>5997
                     /locus_tag="VGT44_06785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580543.1"
                     /translation="MYTLEDDQETSARLRKRDFTDAPTVPVDQGSGGPDAQAPGSPSH
                     THRFSPEQITSSFKKLTTPKQPVANKHDRRVALAALTVACISVFFTALDQTVVVTALP
                     RIIADPGINIPFTQLDHAAWIISAYLLGFVIAMPLMGRVSDIYGRRRILLLCLSIFGI
                     GSIFCGLAPTLGQLVDLSFLNSFGIDTSSPGLIWLISARFVQSIGGGAVVPVAMAIVS
                     DFYGQERRALALGIIGAVTEAGGVMGPLYGALIVQNLGWTYIFYLNAPIVLALMVGAW
                     ILIPRGTRLQEGIDWLGAILLGATLTCLSLGLAQQGTELGPTAVNGAAPQNNPVSLIL
                     AVVFLLAFIVVELIPRWRAPRLLLSRKFPFLRLRRVQETRWPVVDLSLFKRFPFSASS
                     LVSLLVGAALIIAMADIPLFVNTVLSKQINEADMPLVSGLALLRLTAMIPIGAFLGGW
                     LCSRISCRFTGVLGLLFVATGFYLMSRWPMDVDWTQITISTVTTGFGFGLVIAPISTT
                     AINAVRATQAGMGAAIVTSLRMVGMMLGLAALTSWALAYFKQLASQFPSLPPTATTDQ
                     FAKWSQDYANHLINSAHSVYSAVFFTTMILCLIAIIPALFLWGSKQPVETLATVATGP
                     LEDTPTEPDPHVEDTVAVTAAAAGTFLATSDALDNVLLDEPPPLPPVTGDDGSGGNGG
                     RRPRNRRRLLIAIASIALVLLIALGSVFAVFVWQSPASSTTGTSSNPAAG"
BASE COUNT         1383 a         1729 c         1533 g         1352 t
ORIGIN      
        1 cgcgcccgcg cacattatca ttgatcttta caaaaatatt ttcattactg aattgaaaaa
       61 catctgcgtc gcccagcggt atacgcagat gtttgcatat ggatgccgcc agtgggcggt
      121 ttgcatttcc tgtaaagata ctcagatcgt ccattcgctc ctccgggttt gtctgagtat
      181 atcacaccaa ataggtttat gtaaactttg aaacaagctt accgaaagga taaacggacg
      241 ggacttatgt ctctaatgat aagttgtggt acaatagcgt ggattttaga atacatccgc
      301 atttctaaaa ttaactaatt ctcatggaga gtgatgatgt tggaccaatc aaacctgtta
      361 gccggactca acgaaccaca gcaacaagcc gttaccacta ccgaaggacc ggtcctcata
      421 ctggcaggac cgggcagcgg caagacccgc gttatcactc accgcatcgc ttacctcgtg
      481 caacacaatc atgtttcacc ctggcgcatc ctggccgtca ctttcacaaa taaggccgcc
      541 aaagaaatgc gcgagcgcct ggaaaagctg gtgggcatca acgaatcaaa agagatgacc
      601 attggcacgt tccacgccat ttgcgcgcgc gtactgcgca cagaagccga ctatctcgcg
      661 ccaatcggac tcaaccgatc ttttgtgatc ttcgatactg atgaccaggc cgcgcttgtg
      721 aagcaggcca ttaagggcat ggacctcgat gagaaacagt accggcccct aactatacag
      781 gccatgatct cccgcgctaa aaacgacttg cagagtccag atcagatggc cgaactggct
      841 gcgaaatacc tcgaagaggt agccgcccgc gtctataaac aataccagaa gctcttacgc
      901 gccaataacg cggtcgactt cgacgacttg ctcatgctga ccgaacaact ctggcggcgc
      961 gaaccagaag tgctccgcaa gtaccaggca cgctggcact acatccacgt cgacgaattc
     1021 caggattgca acctgcccca atacaagctg attcgcctgc tgggcttcgg cacggacgag
     1081 aggcatgaag ggctgggaaa cgtgtgcgtg gtgggagacg atgaccagat gatttatacc
     1141 tggcgcgggg ccagcgccga aaatgtgctg cgcttcgaac gcgattttcc ccagacaaaa
     1201 atcatcatgc tggaacagaa ttatcgctct acccagacca tcctcgacgc ggcgcagaac
     1261 gtcgtgcagc gcaaccggct gcgcaaagat aagcgtttat ggaccgcgct gggcacaggc
     1321 gagaaaatca ttctgcacga ggcgttcgac gaggaagagg agggactctt cactgcccgc
     1381 gagatcaaac gcttgcaggg acgcggcgat atcgataaac tgggcgacgt cgccgtcatg
     1441 tatcgcacca acgcgcagtc gcgtgccctg gaagaacaat tcctacgcgc aaacattcct
     1501 tataaagtca ttggcagccg caagttctac gaacgtaaag aaatcaagga catgctggcc
     1561 tatctacgct tgctggccaa tccctacgat gacctgagca tgcaacgcat tatcaacgtg
     1621 cctaaccgca aaattggccc caaaacgatg ggtgagttac agcgctgggc cgctgaaaga
     1681 catatcgccc tctacgacgc tattcagcag atcgacgaac accctactct cggtaaggcg
     1741 gcgaaactcg cgttgtcagg ctttggacaa ctgctgcttg acttcaccaa tgccataaag
     1801 gaactgcatt tacccgaact gctcgaccgc attgccgaac gcagcggcta cggcccggaa
     1861 ctgcgcgaga ctcccgaagg tgaagagcgc tggagcaacg tgctggaact tcgccgcgta
     1921 gcggcagact actcggagat cgagacacgt acagcgctcg aactattcct ggagaacgtt
     1981 gcgcttgtcg gcggagcaga tactgcgcaa accggcgaga acggtacgct tataaaggaa
     2041 gagcaaaacg acgcggtaac cttgattacg ctccatgccg cgaaaggtct ggagtatcct
     2101 gtcgtcttta ttgtcggcat ggacgagggc tcgctgcctc atgcgcgttc ggtcgacaaa
     2161 ccagagcagt tggaagaaga acgtcgcctg gcctatgttg gctttacccg cgctatgcgc
     2221 cgcctctacc tcgtgcgtgc gtatcgtcgc tcattctttg gcgaaagcca ggtgaccgaa
     2281 ccttcacgct ttcttgaaga tatccccctg catctcgtct cgaaccaggg tgctgccagt
     2341 tctcactcgc ctggcggctt gcgttcaagc gcgacccggc gcggcagttg gggcgacgat
     2401 tacaactaca accaggatac ccactcgtcg cgcgagactg gccgcgtatt tggttcaggt
     2461 aaaccgcagg ttaacagtca accaccccag cggatggccc gcccatcatc ctacatcgca
     2521 cccaaaacgc cctcaacgct gccttcgcaa aaaggcaacc gcgatgcagc atctccgcca
     2581 tcatcaccaa caacgcctgc tgcccctgca aatccggcca aaccaacgag ccagcagtac
     2641 aagccaggcg atagggttcg tcacgacaaa tttggcgagg gcatcatact taaaagcgaa
     2701 atggaacaaa acaccgagtt cgtcgaagtc caattccagg gcaaacacgg aaaaaaacgc
     2761 ctcagtatgg atttcgcgaa actcgaaaaa ctatgactgt cccaacctgt accataaagt
     2821 accatccagt agcccctgct gcttgattcc ccccaaaggg tctgcaagtt gcgttgattt
     2881 gtttcagcaa gacttttcta ttgactattt ttggggaggc atctttcatg gcttcattcc
     2941 atttcccggt gcgtcgtgca atggtctgtc ttatactcac catagccagc gcgctgttga
     3001 ttgcgggctg cacgcctccg tggcagcatg gtagcgccgc cgttgccaat agtttggctc
     3061 caaaaccgac tgcgcagcag ctactcaaca ctctacaaaa gaacttccgt tcggtcacga
     3121 ctttccatgt gaccttgaaa gtagacaacc tggggactac ttcgggaagt caaatacaga
     3181 ttcgcactgc cgatggcgat gtgatcatgc cagataaagt gaaggcccag gccaatgtac
     3241 tgttctccgg tcagccagtc acagtaaaac tcatcagtat cggtaatatc caatatctca
     3301 ctgaccctat tacaggccag tggcgcgtag tcaagggcgt gctgaatgca agcacgctaa
     3361 cgaatcccga taccggtctt atctctctcg ccactaaatt gcaaaatgta accggcccta
     3421 ccgatgatgc cgttcatggc acaccctgct ggcgcgtcaa cggtatgatg gatgcgaaat
     3481 ccctggcgtt cttcaccggt ggtggcgttc ctgctggcac aatgcttcaa gcgagcatat
     3541 gtgtcgggaa agccgatggg ttgccctacc agttgagcgt agttggtcag gcagccaaaa
     3601 gcgatacgtt gcaaacttca cgcacattca caatctcggg ctataacgag actattagta
     3661 tcaccgcccc tcagttctaa tctcaagtag aacatatgca tgttaatcct gaggtggata
     3721 cagcagtcat tcttgtataa attgttatac atctttttat tggcatttat agcagttctt
     3781 atctttattt cgggggtcag atgtacacat tagaagatga ccaggagaca tccgcgcgac
     3841 tgcgcaaacg tgatttcaca gacgcgccga cagtcccggt ggatcaaggc agtggagggc
     3901 ctgacgcgca agcgcccggc agcccatctc atacccatcg cttctcacca gaacaaatca
     3961 ccagttcgtt taagaagctc accacaccca agcaacctgt tgccaacaag catgacaggc
     4021 gcgtagccct ggcagcgctc actgtcgcct gcatcagcgt ctttttcacg gcgcttgacc
     4081 agaccgttgt tgtcacggct ctaccaagaa tcattgccga cccgggcatt aacatcccat
     4141 tcacgcaatt ggatcacgcg gcgtggatta tcagcgccta tttactcggg tttgtgattg
     4201 ccatgccact gatggggcgc gtatctgaca tttatggacg acgccgcatc ttattgctct
     4261 gcttgagcat ttttggcatc ggctccatct tttgcggcct tgctccaacg cttgggcagc
     4321 ttgtggatct cagctttctg aatagcttcg gcatcgatac ttcctcgccc ggcctgatat
     4381 ggctgatctc cgcacgcttc gtccagtcca ttggcggcgg cgcggttgtg ccggtggcga
     4441 tggccatcgt aagcgacttc tatggtcaag agcggcgcgc cctggcgctg ggcatcattg
     4501 gcgctgttac cgaggcaggc ggcgtgatgg gcccattgta tggtgcgctg attgtgcaga
     4561 acctcggctg gacatatatc ttctatctca atgcaccgat cgtcttagcg ctgatggttg
     4621 gcgcgtggat tctcattccc aggggaactc gcttgcagga aggcatcgac tggcttggtg
     4681 caatcctgtt gggcgcaacg cttacctgcc tgagccttgg actggcccag caaggcacag
     4741 agcttggccc aacggcagtg aacggcgctg ccccgcaaaa taatcccgtc tcattgatcc
     4801 tggccgttgt tttcttgctg gcctttattg tggtggagtt gataccgcgc tggcgcgcgc
     4861 ctcgcctatt gctctccagg aaattcccat tcttgcgttt gaggcgtgtt caagaaacac
     4921 gctggcctgt cgtcgaccta tcgctgttca aacgattccc tttcagtgcc agttcattgg
     4981 tgagcctgct tgtaggcgcg gcgctcatca tcgccatggc agacatcccg cttttcgtca
     5041 ataccgtact cagcaaacag ataaacgaag cggatatgcc gctcgtcagt gggctcgcgc
     5101 tgctccgtct gacagctatg atccccatcg gcgcctttct gggtggctgg ctctgtagtc
     5161 gtatctcctg tcgctttaca ggagtactcg ggctgctctt cgtagccact ggcttctacc
     5221 tgatgagtcg ctggcccatg gatgtcgact ggacgcaaat taccatcagt accgttacca
     5281 ccggcttcgg ttttggcctg gtaatcgctc ccatcagtac aacggccatc aacgccgtgc
     5341 gagcgacaca ggcaggcatg ggcgctgcta tagtgacttc gctgcgcatg gtgggcatga
     5401 tgcttggcct ggctgcactt acctcctggg cattggccta tttcaagcaa ctggcctctc
     5461 agttcccctc actgccgccg acggctacaa ctgaccagtt cgcgaagtgg tcgcaggatt
     5521 atgcaaacca tctgattaac tcggcgcact ctgtctatag cgcggtgttc ttcacaacca
     5581 tgatactttg tcttatcgcg atcatcccgg cgctcttctt gtggggaagc aaacaaccgg
     5641 tagaaacact ggcaacggtc gctaccggac cgctagaaga cactccaact gaacccgacc
     5701 cgcatgtcga ggacaccgtt gcagtcactg ctgccgccgc ggggacattc cttgctacct
     5761 ctgatgcgct cgataatgtc ctcctggatg agccgccccc gcttccgcct gtaaccggcg
     5821 atgatgggag cggcggcaac ggaggccgca ggcctcgaaa tcgccgtcgt ttactgattg
     5881 ccattgccag tatcgcgctc gtgttgttga tagcgctggg tagcgtcttc gccgtctttg
     5941 tgtggcagtc gcccgccagt agtacgacag gtacctcgtc caatcccgcg gcaggct
//
LOCUS       DASZAK010000388         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_541855, whole genome shotgun sequence.
ACCESSION   DASZAK010000388 DASZAK010000000
VERSION     DASZAK010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541855"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            432..1604
                     /locus_tag="VGT44_06790"
     CDS             432..1604
                     /locus_tag="VGT44_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580544.1"
                     /translation="MTTSFVLGKGVTVAAREARVQDALMDFWHDGDNHMETRIYALCT
                     ELSLYGEQFIRFFVSPYDGRVKIRQIDPSIIDQVETDPDDIETPLRFHRRPVGPSATM
                     PGDPAPVTPQDQHNFEGEWFTAGKEVVQFTINKVSNAKRGKSDLATLLPWLRRYKDWL
                     TDRVRINKYKGAFLWDVRLMGADRKTIERKKAEYAYPPEPGSVIIHNEAEQWSAVKPE
                     INANEAAEDGRAIKLMVAVGAQIPEHFLSDGNNGNRATAAEMGLPTFLKFQRRQYVMR
                     AMLRSILDRVLHEARKAGKLPGDIDAHYDIIFPEIDVGDHQTLATAVQQLVSALTTAK
                     AQGWVSDETAMRLLFQFAGEEIDVNEERARIEKEPAACPTTPDPSHPSRSAASPIP"
     gene            complement(1549..1722)
                     /locus_tag="VGT44_06795"
     CDS             complement(1549..1722)
                     /locus_tag="VGT44_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580545.1"
                     /translation="MDLCWRVLVWPLWRAWVIDEASWKLAEANTAKARAAELAVMGLG
                     WLRCGWDGWGLAL"
     gene            1808..>2172
                     /locus_tag="VGT44_06800"
     CDS             1808..>2172
                     /locus_tag="VGT44_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580546.1"
                     /translation="MPLDSRKTQHILNVVPKTFAGRQRTVVLVSLASGVYSFAAIQVI
                     MRPEEVIDPQIYDAGGSQPSRTADMVMIAPLGTTFTGVVYVADTTTATSTAVAAAPKY
                     EIIEALPVGIVPGGSPLRVL"
BASE COUNT          486 a          681 c          637 g          368 t
ORIGIN      
        1 tgcttcgagt ccgacacagg aggattggta ctggggcagg ttggcgacgg ggggcgaaga
       61 agactacttc tggagacgcc taaaatagtg ggccgcttgc tcgtaagggc aagaacgaaa
      121 gctggagaat tcggtgaaag ctgagagtcg ccaacaccga gcggaatttc caagaccatt
      181 cttggttaga cgtgtagaga ctatgcaccg gcctcctgaa atcaggatga agagatagtc
      241 cgaacttacg ggaaatgaac cgtaagagaa tggcagaaat gaccattccg ccagggacct
      301 gtccctggta ggtaacagat tgatcagata actggtacca gaaagacgta atcccctcta
      361 cttacctgga gctgcataac cagtgttacg aggcttacaa cgccaacccg ctcgcctttg
      421 ccatcgtcga aatgacgacc agctttgtgc tgggcaaggg cgtcacggtg gcggcgcgcg
      481 aggcgcgggt gcaagacgcg ctaatggatt tctggcacga cggcgataac cacatggaga
      541 cgcgcatcta cgcgctgtgt acggagctat cgttgtatgg cgagcagttt atccgctttt
      601 tcgtcagccc gtatgatggg cgggtcaaga tccgccagat cgatccgtcc atcatcgacc
      661 aggtggagac cgatccagac gacatcgaga cgccgctgcg tttccaccgc cgaccggtcg
      721 ggccgagcgc gaccatgccg ggcgatcccg cgccggtcac gccgcaggat cagcacaact
      781 tcgagggaga gtggtttacg gcgggcaagg aggtcgtgca gttcaccatc aacaaggtca
      841 gcaacgccaa gcgcggcaag agcgacctcg ctacgctgct gccctggctg cggcgctaca
      901 aggactggct gaccgaccgc gtgcgcatca acaaatacaa aggagcgttt ttgtgggatg
      961 tgcgcctgat gggcgcggac cgcaaaacga tcgagcgtaa gaaggccgag tatgcctacc
     1021 cgccggagcc gggcagcgtc attattcata acgaggcgga gcagtggtcc gccgtcaagc
     1081 cggaaatcaa cgccaacgaa gctgccgagg acgggcgcgc catcaagttg atggtggccg
     1141 tcggcgcgca aattccggaa cattttttga gcgacggcaa caatggcaat cgcgctaccg
     1201 ccgccgaaat gggcctgccg accttcctca aattccagcg ccgccagtac gtcatgcgcg
     1261 ccatgctgcg ctccatcctg gaccgcgtgc tgcacgaggc gcgcaaggcg ggaaaactgc
     1321 ccggcgacat cgacgcacac tacgacatca tcttcccgga gatcgatgtt ggcgatcacc
     1381 agacgctcgc caccgccgtg caacagctcg tctcagccct caccaccgcg aaggcgcagg
     1441 gctgggtcag cgacgaaacc gccatgcgcc tgctcttcca gttcgcaggc gaagaaatcg
     1501 atgtcaacga agaacgcgcg cgcattgaaa aggagccagc agcatgtcct acaacgccag
     1561 accccagcca tcccagccgc agcgcagcca gcccaatccc ataacggcaa gctccgccgc
     1621 gcgcgccttc gccgtgttcg cctccgccaa tttccagctg gcctcgtcga tcacccaggc
     1681 gcgccagagc ggccacacca gcacccgcca gcacaagtcc accgtgcccg tcgtgcatca
     1741 tcccgcccgc gccaaattgc atttgccgcc cggcgtcgaa gaccaaccct tttgacagga
     1801 gattcacatg ccgcttgatt cccgaaaaac acaacacatc ctcaacgtcg tgccaaaaac
     1861 gtttgccggt cgccaacgca cggtcgtgct ggtctcctta gcgagcggag tctatagctt
     1921 cgcggcgatc caggtcatca tgcgcccgga ggaggtaatc gatccacaga tttacgacgc
     1981 gggcggttcg cagccgtcgc gtaccgccga tatggtgatg atcgcgccgc tcggcacaac
     2041 cttcaccggc gtcgtctacg tagcggacac gaccaccgcg acctcaacgg cggtggctgc
     2101 cgcgcctaag tacgagatca tcgaggcgct gcccgtcggc atcgttccag gtgggagccc
     2161 tttgcgcgtg ct
//
LOCUS       DASZAK010000389         4467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_186087, whole genome shotgun sequence.
ACCESSION   DASZAK010000389 DASZAK010000000
VERSION     DASZAK010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186087"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="VGT44_06805"
     CDS             complement(<1..848)
                     /locus_tag="VGT44_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580547.1"
                     /translation="MSQRSSSQFKWPANQSWPSAAQVEWQEPRTFPVESQAQLFPVIT
                     STPDWFFDTSTERYAVIGKQPVRWKKGKLRKQRSKKAKWARRIVVAVSLMTVISLVLG
                     TQLNGEFGAGVADTMRAIFGPTITAQVESWFLGISDKAHQVQYQLGGQQVQAPWSVTH
                     SYQHPVSGTVSQSLMPLVTIKPVISPALPGEGTWVTDGMPPSTGNLPPLVAKTFIRPD
                     ASRPYAIVTLLQFDMRNLALHMIAGTTEPGGARGVRGPGAIPSSDMQQVFAAFNGGFK
                     YADGQFG"
     gene            1501..2559
                     /gene="argC"
                     /locus_tag="VGT44_06810"
     CDS             1501..2559
                     /gene="argC"
                     /locus_tag="VGT44_06810"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEV2580548.1"
                     /translation="MVTVSIVNVSSYTGLELLRMLARHPEFRVTSVTGRSAAGKRLGE
                     VFPQLQGSEPALASLVLKDEADATDLAFLCLPHAAAAEAAVNLLQRGTKVVDLSADFR
                     LRDPAVYEEWYRHVHPAPALLETAVYGLCERYREPMERASLVANPGCHATAAILALIP
                     ALAAGIIGPDLIIDSKTGISGAGRSPTLATHFAEANEDASAYGLDGHRHLPEIIQELA
                     AVAATSANGQTIDKQLRVTFVPHLIPMTRGILATCYADLRSPTMTNAEVRAIYEQYYE
                     GEPFTRVVDTPPHTKWTYGSNLCLLYPTVDRRTGRLVVVSCLDNLVKGASGQAIQNAN
                     RLSGFPETMGIPAMAVFP"
     gene            2581..3453
                     /gene="argB"
                     /locus_tag="VGT44_06815"
     CDS             2581..3453
                     /gene="argB"
                     /locus_tag="VGT44_06815"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEV2580549.1"
                     /translation="MAISISLTAEGDIINSHHLIAEVLREALPYIDYLKGKTLVIKLG
                     GSTLEHQRGVLQDIIWLRSLGACPVLVHGGGPYINAWLEKLNVPAQFEDGLRVTDART
                     LEIVRMVLLGQVNSGLVLMATQMGARAIGLSGSDGNMIQAKIINERLGFVGEITSVDP
                     SIVQTLLDEGYIPVIAPLGQGPDGECLNINADLVAAQLAGALHAEKLIFLSNVVGICR
                     EDGSLIPELSEAEARQLIVDGVVSGGMIPKVTACLDALASVARVHIVDGGEPHVLLRE
                     LFTDQGAGTMIVRA"
     gene            complement(3454..4329)
                     /locus_tag="VGT44_06820"
     CDS             complement(3454..4329)
                     /locus_tag="VGT44_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580550.1"
                     /translation="MSMNWFKQEKNRGYVIAGIGGIVGFIAFYLPYVSYSTTSSSGGF
                     SINGAVSGLIGFSLLITNLVVAVAAVLIFSSTAFGLTTMSYDKQVTYGKYGIVGGGAL
                     VLLLHLIFALGDHSFSVFGVSLSSANGVNSVLSFGFWLAILASLAMIAGGILAIRPSI
                     LSRSASPYPQPYAQPYQQPYPTADQPTPYPSYQGPYPPQSDYLQQYPQPGYPQTGNAP
                     TEMSQQNYQQPGYPQQSAPLSQQNYQQPPVNPPTHSAPTEMAQQHYQQQQGIQQPQYP
                     PQQPLQQPPQRQTGQ"
BASE COUNT          938 a         1318 c         1292 g          919 t
ORIGIN      
        1 ccaaactgcc catcagcata cttgaacccg ccgttgaagg ccgcgaagac ctgctgcata
       61 tcgctcgatg gaatggcgcc gggacccctc acgccccgcg ccccgcccgg ttcggttgtg
      121 cctgcaatca tatgcagcgc caggttgcgc atgtcgaact gcaacagcgt gacaatcgca
      181 tagggacgcg acgcatcggg cctgatgaag gtttttgcca ccagcggcgg cagattgccg
      241 gtcgaggggg gcataccatc cgtcacccac gtcccttcgc cgggcagagc gggcgaaatc
      301 accggcttga tcgttacaag cggcataaga gactggctga ctgtaccact aacggggtgt
      361 tgataggagt gagtcaccga ccagggtgcc tgtacctgct ggccgccgag ttgatactgc
      421 acctggtgcg ccttatccga aatgcccagg aaccacgact ccacttgcgc ggtaatcgtc
      481 ggcccgaaga tagcgcgcat tgtatcggca acccccgcgc cgaactcccc attcaactgc
      541 gttcccagca caagactgat aaccgtcata aggctcacag caacaacgat gcgacgcgcc
      601 cacttagcct tcttgctgcg ttgtttgcgc agcttacctt tcttccatcg gactggctgc
      661 tttcctatga ctgcgtaccg ttccgtagat gtatcgaaga accagtccgg cgtactcgta
      721 atcaccggaa acagttgtgc ctgcgactcc accgggaacg tgcgcggctc ctgccactct
      781 acctgcgccg ctgatggcca ggattgattg gccggccatt taaactgact gctgctgcgt
      841 tgtgacatgc cctgggcctg ctgctgctca ttcaaattcg tggatacacc ccctgctaaa
      901 cataacgatt ttactcacaa agcataaccc ctacctgtga gaaatgcatt aaaggttgga
      961 attgttaacc cacttgtcct atgcagcggt gaagaaagcc agggcgatgc tggccatcgc
     1021 cctggctttc ttgtgtcaca gcggttgact tttcgcgctc ctggtgttac tgttattaat
     1081 acaatctata aaggctgacc cgcgacgacc gaagaagtat gagtgtggcg accagcagcg
     1141 aaccggcgca tggtggaagg ccggaagggt gcgcactctc agaaccgcct cgcgagcggc
     1201 tctccgaaga aagctccgct gtatgcgcag caagtaggaa aggtggctcg gttcccacgc
     1261 agcgcccgaa ccggaataag tggtcttttc accgttacta cactgtcgta ggtcaggtgt
     1321 caccaggact ggtgcgtccc gtcggatggc gggatcgaaa gacaagcagg gtggtaccac
     1381 gggagttcgt gtaggtatcc aggcaataca caactcgctc tcgtcccttt ctcgtgtgag
     1441 attgggatgc agggcgtttt tttatggccg tttgtttctg gcacaaggag aagatgagag
     1501 atggtcacgg tatccatcgt caatgtgagc agctataccg ggttggaatt gctgcgtatg
     1561 ctcgcgcgcc atcccgaatt tcgcgtgacc agcgtcaccg ggcgcagcgc ggcgggcaag
     1621 cggttgggag aggtctttcc gcagctccag ggcagcgaac cggcgctcgc ctcactcgtc
     1681 ttgaaggatg aggccgacgc gaccgacctg gcctttctct gcctgcccca cgcggcggcg
     1741 gcagaggcag ccgtgaacct cttgcagcgc ggcacaaagg tagtggacct ctcggctgat
     1801 ttccgcctgc gcgatcccgc cgtctacgag gaatggtacc ggcatgtcca tcccgcgccc
     1861 gccctgctgg aaacggcggt gtacggcctt tgcgagcgct accgcgagcc gatggagcga
     1921 gcctcgctgg tcgccaaccc cggctgccac gccacagcgg ccatcctggc cctcataccc
     1981 gcgctggcgg ctggtatcat cggacccgat cttatcatcg actccaaaac cggcatcagc
     2041 ggcgcgggtc gcagcccgac gctggcaacc catttcgccg aggccaacga ggatgccagc
     2101 gcctatggtc tcgacggaca tcgccacctg ccagaaatca tacaggaact ggcggctgtg
     2161 gcagcgacga gcgcaaacgg tcagactatc gataagcagt tgcgcgtcac ctttgtcccg
     2221 catctaatac ccatgacacg cggtatcctg gcgacctgct acgccgacct gcgctcgccc
     2281 accatgacga acgccgaagt gcgcgcgatc tacgagcaat actacgaggg cgagccattc
     2341 acgcgggtcg tggacacgcc gccgcacaca aaatggacgt atggcagcaa tctctgcctg
     2401 ctgtatccga ctgtagaccg gcgcacaggc cgcctggtcg tcgtttcctg cctggataat
     2461 ctcgttaaag gcgcgtcggg ccaggctata cagaacgcca accgtctctc tggcttcccc
     2521 gaaacgatgg ggattccagc gatggcggtg tttccataaa gaaagagaga agaagaagca
     2581 atggcaattt caatttcgtt aactgcggag ggcgacatta tcaatagtca ccacttgatc
     2641 gcggaagtgc tgcgcgaggc gctgccttat atcgactacc tgaaaggcaa aacgctggtc
     2701 atcaagctgg gcggcagtac gctcgaacac cagcgcggcg tgttgcagga tatcatctgg
     2761 ctgcgcagcc tgggagcctg ccccgtgctg gtgcatggcg gcggccccta catcaacgcc
     2821 tggctcgaaa agctcaacgt tcccgcgcaa ttcgaagatg gtctgcgtgt cacggatgct
     2881 cgcacgctcg agatcgtgcg catggtgctg ttggggcagg tcaactccgg tctggtgctg
     2941 atggcgacac agatgggcgc gcgcgcaatc gggctgagcg gcagcgacgg caacatgatc
     3001 caggcgaaga tcatcaacga gcgcctggga ttcgtaggcg agatcacgtc ggttgacccc
     3061 tcgatcgtcc agacgctgct cgatgaaggc tatatccccg tcatcgcccc gcttggccag
     3121 ggacccgatg gtgaatgcct gaatatcaat gccgacctcg ttgcggcgca actggcgggt
     3181 gcgctccatg ccgagaagct catcttcctc agcaatgtcg tcggcatctg ccgtgaagac
     3241 ggttcgctca tccccgaatt aagcgaggcc gaggccaggc aactgatcgt cgatggtgtc
     3301 gtcagcggcg gcatgatccc gaaagttacc gcctgcctgg atgccctcgc ctccgtcgcg
     3361 cgcgtgcata tcgtcgatgg cggcgaaccg cacgtgctgc tgcgcgaact cttcaccgac
     3421 cagggggccg gcacaatgat cgtcagagcg taattactgg cccgtctgcc gctgcggtgg
     3481 ttgctgcaga ggctgctgtg gtggatattg cggttgttga ataccctgct gctgttgata
     3541 atgctgctgc gccatctccg tcggtgcgct gtgcgtcggc ggattgaccg gtggttgctg
     3601 gtaattctgt tgcgacagcg gcgcagattg ctgcgggtag cccggttgtt ggtagttctg
     3661 ctgtgacatc tccgttggcg catttcccgt ctgtggatag cccggctgcg ggtactgttg
     3721 tagataatct gactgcggcg gatagggtcc ctggtacgac ggatacggag tcggctggtc
     3781 tgctgtagga tagggctgct ggtagggttg tgcatacggt tgcgggtatg ggctggcgct
     3841 tcttgatagg atagaagggc gaatcgcaag tatgccgcct gcaatcatcg cgagcgaggc
     3901 cagtattgcg agccagaaac cgaagctcag gacgctgttg accccgtttg ccgaggagag
     3961 actcacacca aacacgctga agctatgatc tcccagggca aaaatgagat gcaacagcaa
     4021 caccagtgcc ccaccaccaa cgatgccata cttaccatac gtaacttgct tatcataaga
     4081 catagtcgtc agaccaaagg cggtactgct gaagatcaaa acggcggcaa cagctacaac
     4141 aagattggta atcaacaaag aaaacccgat taacccggag accgctccgt ttattgagaa
     4201 accgccgctt gagctggttg tcgaatacga gacatacggc agataaaagg cgataaaccc
     4261 cacaatgccg ccgatgcccg cgatgacata tcctcgattc ttctcctgct taaaccagtt
     4321 catactcata accaggccaa cctttctcgc aatataggta tacttgttcg ctcttaacta
     4381 tactgcaagt cctgcctgat tacaagcggc cctcctacta cattatccag ggtgatgtgg
     4441 cgtgtagggg cgcaattcat tgcgccc
//
LOCUS       DASZAK010000390         2775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_186193, whole genome shotgun sequence.
ACCESSION   DASZAK010000390 DASZAK010000000
VERSION     DASZAK010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186193"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..636)
                     /gene="thpR"
                     /locus_tag="VGT44_06825"
     CDS             complement(49..636)
                     /gene="thpR"
                     /locus_tag="VGT44_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEV2580551.1"
                     /translation="MTRTFIALEMVETLQHYLGDLIAYAARELPALRWVDAAGIHLTL
                     AFLGELNDDRLAAAMEATRAAAQDVPDFTYSMKGLGIFGSPQQPRVIWMGVEDQPSPY
                     VQGSPLQLAHRVLNRELERRQFEVEKRPFSPHLTLAHVKQPLSPLEQQALQRLLQSKR
                     AAPPTQRYAAGRLAVMKSELSRGGAKYTCLQEYAL"
     gene            complement(633..2387)
                     /gene="ftsH"
                     /locus_tag="VGT44_06830"
     CDS             complement(633..2387)
                     /gene="ftsH"
                     /locus_tag="VGT44_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV2580552.1"
                     /translation="MRIALPALIAMVVVLVGVFVFHFAAGGISRVNTLEEPISSVLNM
                     ADRHQLASVTINGDDVIAVGVNGQSYHAVKETNQSVTDLLRHDGVVVSVDSGQQSQWS
                     QIVVDFLFLALVGVVLYFFFRRSSAGGQAGVFTRSRARRFQESRPTLLFRDVAGVEEA
                     KMELEEIVEYLKAPSRFTAMGARVPKGVLLVGAPGTGKTLMSRAVAGEANVAFYSISG
                     SEFVEMFVGVGAARVRDLFKEAKEHAPCIVFIDEIDAVGRQRNATGTGGNDEREQTLN
                     QLLVEMDGFDKQTNVVVIAATNRPDVLDPALLRPGRFDRQVMLDKPDIRGRQAILEVH
                     AQGKPLASDVTMVGLARQTVGFSGADLANLLNEAALLAARRHKEAIEQPDLDEAILRV
                     MAGPERKSRVISDAEKAIIAYHEVGHAIVMRSIPGADPVQKVTAIARGMALGITVQAP
                     IEDRYLMRKSELLAKLAGAMGGRAAEELIFGDITTGAAQDIEYVTTVARRMVCEFGMS
                     PLGLLSLKKEGDGGASISADTAARIDREIAALVDAAYATALDILRERQEKLVAIATHL
                     MEVETMDGAELDAMLNAA"
BASE COUNT          563 a          872 c          754 g          586 t
ORIGIN      
        1 agtggcgcac ccttgcggtc gccatgtctt cgcggtcgcc ctggacagct aaagcgcata
       61 ttcctgcaag cacgtatatt tcgccccgcc gcgcgatagc tcgctcttca tgacggcaag
      121 acgccctgcc gcatatcgtt gtgtgggcgg ggcggctcgc ttcgattgga gcaggcgctg
      181 cagcgcctgc tgctccaacg gggacagtgg ttgtttaaca tgagccagtg tcaggtgcgg
      241 cgagaagggg cgtttctcga cctcgaactg ccgccgttcg agttcgcggt tgaggacgcg
      301 atgcgccagt tgcagaggcg atccctgcac gtagggcgag ggttgatcct ccacacccat
      361 ccagatcacg cgcggctgct gtggcgagcc gaagatgccc aggcccttca tgctataggt
      421 gaagtcaggg acatcttgcg ccgcggcgcg cgtggcctcc atcgccgccg ccaatcgatc
      481 atcgttcaat tcgccgagga aggccagcgt caggtggatg cccgccgcgt caacccagcg
      541 caacgcgggc agttcccgcg ctgcataagc aatcagatcg cccaggtaat gttgcagagt
      601 ttcgaccatt tcaagggcga taaacgtgcg tgtcatgccg cgttgagcat ggcgtcaagc
      661 tccgcgccat ccattgtttc gacctccatc aagtgtgtgg cgatggcgac cagcttctcc
      721 tgcctctcgc gcaggatgtc cagcgcggtc gcataagccg cgtcgaccag cgccgctatc
      781 tcccggtcga tgcgcgccgc cgtatcggcg ctaatagaag cgcccccatc tccttctttc
      841 ttgagcgaca gcaggccgag cggactcatc ccaaattcgc aaaccatgcg gcgggcaacg
      901 gtcgtgacat actcgatatc ctgcgccgcg ccggtggtga tatcgccaaa gatcagttcc
      961 tcggcggcgc gccctcccat cgcgccggcc agcttcgcca gcaattcaga tttgcgcatg
     1021 aggtagcgat cttcgattgg cgcctgcaca gtaatgccaa gcgccatacc gcgcgcgatt
     1081 gcggtgactt tttgcacggg atcagcccct gggatcgagc gcatgacgat ggcgtgtccc
     1141 acctcgtgat aggcgatgat agccttctca gcgtccgaaa tcacgcggct tttgcgttcc
     1201 ggcccagcca tcacgcgcag gatggcctcg tccaggtcgg gctgctcgat cgcctcttta
     1261 tgacggcggg ccgccagcag cgccgcttcg ttcagcagat tggcaagatc agcgccggag
     1321 aagccgaccg tttgccgcgc cagccccacc atcgtaacat cgctcgccag tggcttgccc
     1381 tgcgcgtgta cctccaggat ggcctggcga ccgcgaatgt cgggcttatc gagcatgacc
     1441 tgccggtcga aacggcctgg acgcagcagc gcaggatcaa gtacgtcagg gcggttggtc
     1501 gcggcaatca cgactacgtt ggtctgctta tcgaacccgt ccatctcaac aagcagctgg
     1561 ttcagcgtct gctcgcgctc atcgttgcca cctgtaccgg tagcgttacg ctggcggcca
     1621 acggcatcga tttcatcgat gaagacgatg catggcgcat gctccttcgc ctctttaaag
     1681 agatcacgca cccgcgccgc gcccacgccg acgaacatct cgacaaactc agagccgctg
     1741 atgctataaa atgccacatt cgcctcgcct gccacggcac gcgacatgag cgtttttccg
     1801 gttcccggcg cgcccaccag cagcacccct ttcgggacgc gcgcgcccat cgccgtaaag
     1861 cgcgaggggg ctttgagata ttccacaatc tcttccaact ccattttcgc ctcttccacc
     1921 cccgctacat ccctaaaaag aagcgttggt cgcgattcct ggaaacggcg agcgcgcgag
     1981 cgggtaaaca cacccgcctg gccgccggca ctgcttcgcc tgaaaaagaa gtataggacg
     2041 acacccacca gagccaggaa gaggaagtct accactatct gcgaccattg gctctgctgc
     2101 ccgctatcga cgctgacaac caccccgtca tgccgcaaca ggtcggtcac ggattggtta
     2161 gtttccttca ccgcatgata gctctggcca ttaacgccga cagcaatgac atcatcacca
     2221 ttaatcgtta ccgatgctag ttgatggcgg tctgccatat tgagaacact gctgattggc
     2281 tcctccagcg tattgacacg gcttatcccg cctgcagcga aatgaaatac gaagactccc
     2341 accagcacta ctaccatcgc gatcagcgcc ggaagagcga ttcgcatcca tctatttcgc
     2401 tttctggatg ttctctgcgt cgaacgcgaa gtatgtgtac gagtcgattt gggttcaaca
     2461 ggctcctggt tgtcgtgttg aggcgcttgc atgaaataac ttctcaccct tctaaggctc
     2521 tggcacgcca ttgtgccgac catctgccac ctgcgctgta gcacttacat aggaagagac
     2581 cccttgcccc caggcaaagg gcttcaaaat atatcacgct ttactattgt gtttcaaaat
     2641 gctgcatgat gctagaataa ctcatacttt ccttagaatc aagtgtattg tttagttggc
     2701 aaaaatgtag ccaaaatgta cacatggggt taattgagga aggaatctgg cctcttttgt
     2761 tccgaataca cgaac
//
LOCUS       DASZAK010000391        10336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_436404, whole genome shotgun sequence.
ACCESSION   DASZAK010000391 DASZAK010000000
VERSION     DASZAK010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 10336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10336
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436404"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1344)
                     /gene="serS"
                     /locus_tag="VGT44_06835"
     CDS             complement(61..1344)
                     /gene="serS"
                     /locus_tag="VGT44_06835"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV2580553.1"
                     /translation="MLSLDFIRQHPDVVREGLRRRDETQDIDAILRLAEQRRGLVTRC
                     DGLYAELKRLHERVRLTPEEQREALNRQIKAISRDIRQLEVQCTDIDTHLQPLLLALP
                     NPPHTSVPSDEDEQDRELRRWGEPLEAPGFALQPHWEISERLGLTDFAAGAKIGGSRF
                     VALKGLGARLERALITFMLDLHTREHGYTEILPPFLVKRSLMTGAGQFPKFEDQVYAC
                     TEDELYLNPTAEVPLVGLHSDSILPPGTLPLRYVAWTTAFRRETGSTSRQTRGLLRLH
                     QFNKVELFQLVEPSHSYAVLDAIIAHAERVLRLLDLPYRVVALHARNLPFASAKTVDL
                     EVWMPAQDRYIEISSISNCEAFQAQRADIKYRPDASSRSEYVHTLNGSGLAVGRTMAA
                     LLETYQQADGSVVIPKVLRPYMAMSALPFSKNWRV"
     gene            1422..1814
                     /locus_tag="VGT44_06840"
     CDS             1422..1814
                     /locus_tag="VGT44_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2580554.1"
                     /translation="MIAITFKRNAASPPQSICDLRESVGWDRSEEDYPAAFSGYWGTV
                     SGFDSTGKLVAWCAILSDGVRHAVLIDVIVRQAWQRQGIGLALVKEAIAFIQEQGITI
                     IHVDFLPEHQAFYEKCGFQTSLAGIIEK"
     gene            1979..2548
                     /locus_tag="VGT44_06845"
     CDS             1979..2548
                     /locus_tag="VGT44_06845"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEV2580555.1"
                     /translation="MIPADDTLGPGQGHWTYETYAALPHDDHRYEIVDGVLYMTPAPS
                     RGHQIATGRLYRYLSMHIEDTRLGEVYIAPFDVELGSRTVVQPDVIVVLNAHLNRVTE
                     SRIIGSPDLVVEVSSPGTAGHDRDEKKHAYARAGVPEYWIVDPWSRTIEVLTLDADSY
                     SSQGVFEGKAVLPSSIIPHFPVHVEQFFA"
     gene            2682..3149
                     /locus_tag="VGT44_06850"
     CDS             2682..3149
                     /locus_tag="VGT44_06850"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV2580556.1"
                     /translation="MITVVKLNPPGEPKITYTGEVAWHVPNGVVISAVWRHPPKDLGY
                     ARFEPGDHFTEYYYSDRWYNIFDIVSAVGERKGWYCNVAQPAMIFEDRIEQVDLLLDV
                     WVDRQGVPLVLDEDEFETDTSMSDEQRAGARQGLQDLLQVIAAQDEPFRKDKS"
     gene            3149..3583
                     /locus_tag="VGT44_06855"
     CDS             3149..3583
                     /locus_tag="VGT44_06855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580557.1"
                     /translation="MEKKGARLFQQGEMPFDRNIVELDTRRQLRLKVNELIRLVNDPM
                     LTTSEIQAHLLHGAATYGNLFAAQLVRSLHRNDLQERQSLVWLLTLLNDETTIPLLSR
                     MSRNVQMPRPVRLSASLALAGLGATTALIEANSRRPRLYAIS"
     gene            3593..4054
                     /locus_tag="VGT44_06860"
     CDS             3593..4054
                     /locus_tag="VGT44_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2580558.1"
                     /translation="MIQLDIPGKGFIELFHAVFDINGTLAVDGHPFPEVVERLQALAE
                     LVSIDALTAGTHGNLAELERTLGLPLRRISTGEDKARFVEQLGPARVVAIGNGANDAP
                     MLRLAAIGIAVLSSEGVSSAALQAADVLAQGPVDAIDLLLRPKRLIASLRT"
     gene            4246..5175
                     /locus_tag="VGT44_06865"
     CDS             4246..5175
                     /locus_tag="VGT44_06865"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV2580559.1"
                     /translation="MARIEQPGSTETRPEAQPVQQSKLGFLYVCVAVFFFSTSPIFVR
                     WSSPFSSIEVAFWRLAIAALLVAAMGVITRQRLLLRRQEVPRFLLYGLVTALHFIFYI
                     GSLSFTTIAHALALIYTSPIFVTLFSAIVLREPLPRRKYLGIAVAIVGIAIMAGFDPH
                     YTACSLAGTGHCMALGDGMALLSGICYAIYSVAGRGERDRHPLFRYTTNVYGFAALWL
                     LPATLYLAFTHAYPLAAIGAVAALGIFPLGLGHTLYNAAVRRVHATYANLIATQEVTG
                     GIILGIFLLGEIPSLVTVIGVIVALVGIAGVLV"
     gene            complement(5182..6009)
                     /locus_tag="VGT44_06870"
     CDS             complement(5182..6009)
                     /locus_tag="VGT44_06870"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEV2580560.1"
                     /translation="MYDYRIFGLDFTSTPSARKPITCATCTIKGTTLHLDECRSLATL
                     DDFDAFLRADGPWLAALDFPFGQPRALIEQLGWPTIWCDYMKFLASMDKTTFVAMLQG
                     FMNSQPPGAKLLLRATDILASARSPMMLHRVPVGKMFFGGATRLFRSSVSVLPCRPTS
                     DNRVAIEGYPVLVARRLIGKRSYKSDERGKQTREQVQARHEIVHGLRSALLMDTYGLA
                     VEMSDDVAESLAQDAMGDRLDAVLCALQAGWAYLERGRGYGISKECDKDEGWIVDPL"
     gene            complement(6028..6603)
                     /locus_tag="VGT44_06875"
     CDS             complement(6028..6603)
                     /locus_tag="VGT44_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEV2580561.1"
                     /translation="MALINIATHEIHCKIVYYGIGYCGKTTNLQYVFKSINPSVRGEM
                     LSIATETERTLFFDFLPLDLGTVHGFRTRFHLYTVPGQILYERTRMAVLNGADGIVFV
                     VDSQAEKFEENIQSISELEMNLRHLGKDVATFPFVVQWNKRDMPSALPTNVLERYLNR
                     RRVPSFEAIASSGTGVYATLRSISKRVMAQL"
     gene            complement(6617..7111)
                     /locus_tag="VGT44_06880"
     CDS             complement(6617..7111)
                     /locus_tag="VGT44_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEV2580562.1"
                     /translation="MEQLTNLIISDQEIVFISNALSKLMNDTSASSAMLIDKSGQVIV
                     TQGATTRRNATSLGALLAGAFSSSRQIAELLGEKDFRSIFQQGVHQNIFTTMIEDQWL
                     LVIVFDKLTHIGLVKVLSKKASDELAHTLERVKADTTRQKASVLNIQFRSSVEDTIDL
                     LFRD"
     gene            complement(7211..7576)
                     /locus_tag="VGT44_06885"
     CDS             complement(7211..7576)
                     /locus_tag="VGT44_06885"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEV2580563.1"
                     /translation="MVELKQSLSRFLTIPGVRLAVLVGRDGLMIEGLAREGKEDLEAI
                     GAIMTSGLNSAEALSQEITRGSVVGVLNEYERGLVSVDPMGDFAVMVTLFDNAASIGR
                     VRHMVKTTRTDILDALDMK"
     gene            7853..8950
                     /locus_tag="VGT44_06890"
     CDS             7853..8950
                     /locus_tag="VGT44_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2580564.1"
                     /translation="MTKKLTIRDIAGLADVSTATVSRVLNQKPDVDPATRERILRIME
                     EQSFMPNIAASGLAGRRSHLLGVLTPSFTWPLIPELMRGISEVVDSTSYELILYSITD
                     ANDKDPSDVIDRIVRTRLAAGLLAVFPGLSTRHLTELNSRNFPVILIDDQGIPPEGTP
                     WISVENRPGAYEATRHLIRLGHRRIAHIQGPLRYQVSHDRYMGYCDALNEAGIAPDPI
                     LVREGDFLPRGGYISSNVFFDLPAEQRPTAIFAASDYMAYGAISAAEQRGLSVPEDMA
                     VVGFDDNPTSAHMEPALTTVRQPFYELGRRATEMLLALVETPRYVNGLNHNGRVLGAP
                     SSTASESIRIKLPTSLIVRTSCGASRGVTMV"
     gene            9209..>10336
                     /locus_tag="VGT44_06895"
     CDS             9209..>10336
                     /locus_tag="VGT44_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2580565.1"
                     /translation="MTPNLASSPRRNRRATVFFSLLLPLVMLLAACGGTTPTTTTSNH
                     ATTLRVQWAPGQPVPDLFNPFFNANQGGAWGAQGLLYDELYFTNLYNGQHTPWLANSY
                     QYNSDLTQLTFTIRPGVKWNDGQPFTSADVLFTFNLMQAHPALDQNSIWASTLKSLAA
                     PDPNTVVFTLQAPDSTALFYIGGTFIVPQHVSGWTTGDPAKFANDKNPVGTGPYLLSS
                     FNSDQITYTRNPNFWGNSVGMTPQVQTIQAVATKDNTTTITNMIQGQYDWTGVGWSPD
                     FDSQYKAKDPQHNQDWFAASNTVMLYLNLQKAPFNNLLVRKAISAAINRDQLPQGVAL
                     YAKVANPTGVIIPNLNSWIASQYQSAGFQYSASQAGSYLQQA"
BASE COUNT         2182 a         2861 c         2925 g         2368 t
ORIGIN      
        1 ccagctgttt tcaagacagc cgcaatagac cactctgcca tccctccaat ggctactata
       61 tcatactcgc cagttctttg aaaaggggag ggctgacata gccatatatg gccgtaggac
      121 ttttgggata accacagagc cgtcggcttg ctggtaagtt tcgaggagcg ccgccatggt
      181 gcgcccaacg gccaggccgg agccgttcag cgtgtgaaca tactccgagc ggctgctggc
      241 gtcgggacga tatttgatgt cggcgcgttg ggcctggaac gcctcgcaat tgctgatgga
      301 agaaatttca atatagcgat cttgagcggg catccagacc tccaggtcaa cggttttcgc
      361 ggaagcaaag ggaaggttgc gcgcgtggag cgcgaccacg cgatagggca ggtcgagcag
      421 gcgcagcacc cgttcagcgt gagcgatgat cgcgtcgaga accgcgtaag agtgcgacgg
      481 ctcaaccagt tgaaacagct ctaccttatt gaactggtgc aggcgcagca ggccgcgcgt
      541 ctggcgtgaa gtcgaccccg tctcgcgtcg gaaggcggtc gtccaggcga cgtagcgcag
      601 gggcaatgtg ccgggcggca ggatagaatc gctgtgcagt ccaaccagcg gaacctcggc
      661 ggtagggttg agataaagtt catcctcggt gcaggcgtag acctggtcct cgaacttggg
      721 aaactggccc gcgccggtca tcaacgaacg cttgaccaga aaaggcggga gtatctcggt
      781 atagccatgc tcgcgggtgt gcaggtcgag cataaaagta atcagcgcgc gttctaaccg
      841 cgcgccaaga cctttcagcg cgacaaagcg gcttccgccg atctttgccc ctgccgcaaa
      901 atcggtcaga cccaggcgct cgcttatctc ccaatgcggt tgcagcgcga agccaggcgc
      961 ctccaacggc tctccccagc ggcgaagctc gcggtcctgt tcgtcctcat cagatggaac
     1021 gctggtgtgc ggtggattgg gtaaggccag caggagcggc tgcaagtgcg tatcgatgtc
     1081 ggtacattgc acttcaagct ggcggatatc gcgcgatatg gccttaatct gcctgttcag
     1141 cgcctcgcgc tgttcctccg gggtaagtcg cacccgctcg tgcaggcgct tcagttcggc
     1201 gtacaggccg tcgcagcgcg tcaccagccc ccggcgctgc tcggcgaggc gcaggatagc
     1261 gtcgatgtcc tgtgtttcgt cgcggcggcg cagcccctcg cgcacgacat cgggatgttg
     1321 gcgaatgaaa tccaggctta acatatcgga cacagtatag cacaggtgta tagcgtttga
     1381 tgcatgctga tacacctgtg gggcatgata taatcactac catgattgct attactttca
     1441 aacgcaacgc cgcttccccg cctcaaagca tctgcgactt gcgtgagtcg gtcggctggg
     1501 accgctcgga ggaagactat cccgccgcgt tttcaggcta ttggggaaca gtgagcggct
     1561 tcgattccac cgggaaactg gtcgcgtggt gtgctatcct cagcgatggt gtgcggcatg
     1621 ccgtgctgat tgatgtgatt gtgcgtcagg catggcagcg acagggcatc gggttggcac
     1681 ttgtcaagga ggccatcgca tttatacagg agcagggtat tacgattatc cacgtcgatt
     1741 tcttgccgga gcatcaggca ttctatgaga aatgcggatt tcagaccagc ctggcaggca
     1801 tcatagagaa atgagtagag tgagaaatta atggctacct cttgtgaacg tatatgctct
     1861 atgctactat agaaccgtag tgatgaataa tctctaactc atggaagaat cgtaccaacc
     1921 aagaagtgca taaggacaca aagttatgac gatgacatcc cctgggaggg ttccgatgat
     1981 gatacctgct gacgatacac tcggaccagg gcagggacat tggacatatg agacgtatgc
     2041 agctcttcct catgacgatc atcgctacga gatcgtggat ggagtgttat atatgactcc
     2101 tgctcccagt agaggacacc agatagcaac tggtcgtctg taccgatacc tctcgatgca
     2161 cattgaagat accaggcttg gagaagtata tattgcgcca tttgatgtcg aattaggctc
     2221 gcgaaccgtt gtgcagccgg atgtcattgt ggttctgaac gcgcatctca atagagtgac
     2281 agagtcacgc attattggct cgcctgatct tgtcgttgaa gtttcctccc ctggtactgc
     2341 tggacatgat cgagacgaaa aaaagcatgc ctatgctcgc gcgggcgtgc cagaatattg
     2401 gatcgtcgat ccatggtcac gcacgatcga agtacttacg ctcgatgccg atagttattc
     2461 ttcccagggt gtttttgagg gaaaagcggt tttaccctcg agcatcatcc cccattttcc
     2521 ggtgcatgtc gagcagtttt tcgcctgatt actattgaag gatttgtatt ctatgttggt
     2581 ctgagcatga ttctgcctgg ttggttgctt gcctgcgaaa tatatttgca taggacgctc
     2641 aaataatgta cactatcagt tggtgtccag gagcaagagg catgataacc gtagtcaagt
     2701 tgaatccgcc tggcgagccg aagatcacgt atacgggcga ggtcgcctgg cacgtaccca
     2761 acggtgttgt catatcagcg gtgtggaggc atccgccgaa agacctcggg tacgcgcgct
     2821 ttgagccggg agaccatttt accgaatatt attatagtga ccgctggtat aacatcttcg
     2881 atatcgtctc agccgttgga gagcgcaagg gttggtattg taacgtcgcg cagcctgcca
     2941 tgatcttcga ggatcgcatc gagcaggttg atttgctgct tgatgtgtgg gttgaccggc
     3001 agggtgtgcc gctcgttttg gatgaggacg agtttgagac cgatacgagc atgagcgacg
     3061 agcagcgcgc aggggcgcgc caggggctac aggacctgtt acaggtgatt gcggcgcaag
     3121 atgagccgtt caggaaggac aaatcttgat ggagaaaaaa ggcgccaggc tgttccagca
     3181 gggtgaaatg ccctttgatc ggaacattgt ggagcttgat acacgcagac aattacgtct
     3241 gaaggtcaat gagctaattc gacttgtcaa cgatcccatg cttactacca gcgagattca
     3301 agcgcatctc ctccacggcg cagcgacata cgggaatctc ttcgctgccc agcttgtgcg
     3361 ttcgctccat cgcaacgatc tccaggaacg ccagtctctt gtctggctat tgaccctgct
     3421 gaacgacgag accaccatcc cgctgctttc gcgcatgtcg cgtaatgtac agatgcctcg
     3481 ccccgtgcgt ctctccgcct cgcttgccct ggcaggcctg ggcgcaacaa cggcgcttat
     3541 cgaggcgaac agcaggcgac cacgcctgta tgccatcagt tagagtaaga gcatgatcca
     3601 gctagatatt ccaggcaaag gctttatcga actttttcac gcggtattcg atattaacgg
     3661 cacgcttgcg gtagatggcc acccctttcc tgaagtcgtc gagcggctgc aagccctggc
     3721 cgagctggta tccatcgacg cgctgaccgc gggtacgcac ggcaacctgg ccgaacttga
     3781 gcgcacgctt ggcctgccgc tgcggcgtat cagcactggc gaggataaag cgcgttttgt
     3841 tgagcagctt ggccccgccc gcgtcgtcgc catcggcaac ggcgccaacg atgcccccat
     3901 gctgcggctg gccgccatcg gcattgcggt actatcaagc gaaggcgtct ccagcgccgc
     3961 tttgcaggcc gccgatgtgc tggctcaggg cccggtggac gccatcgacc tgttgttgcg
     4021 cccgaaacgc ctgatcgcgt ccctgcgaac atgataccaa taccgttgta ggacgcaatg
     4081 atgattttta agaaacatga acggtcaaag gttgtcaccc tgagcgcagc gaagggtccc
     4141 ggtggatggg ctgaaagatc cttcgctgcg ctcaggatga caagattggt taggaatatt
     4201 catcatctcg tccctacagc aggatactaa gatagagtta ttttcatggc cagaatcgag
     4261 cagcctggat caactgaaac ccggccagag gcgcagccgg tgcagcagtc aaaactgggc
     4321 tttctgtacg tttgtgtcgc cgtcttcttc ttctccacca gccccatttt cgtgcgctgg
     4381 tcgtcgccct tttcctctat cgaggttgcc ttctggcggc tggccattgc cgcactgctg
     4441 gtagctgcaa tgggcgtcat cacgcgccag cgtttgctct tgaggcggca agaagttccc
     4501 cgtttccttt tgtatgggct ggtgacagcg ctgcacttca tcttctacat cggctcgctt
     4561 agcttcacta cgattgctca cgcactcgcc ctgatctata cctcgcccat cttcgtcacg
     4621 ctattttctg ccatcgtgct gcgcgagcca ttgccccggc gcaaatacct cggcatcgcg
     4681 gtcgccatcg tcggtatcgc catcatggcc ggtttcgatc cgcactatac cgcctgctcg
     4741 ctggctggca ctggtcactg tatggcgctg ggcgatggca tggcgctgct gtcgggcatc
     4801 tgctacgcta tctactccgt cgcaggcagg ggagaacgcg accggcaccc gctcttccgc
     4861 tatacgacca acgtgtatgg cttcgcggcg ctgtggctac tgccagccac gctctacctc
     4921 gccttcaccc atgcctaccc gctggcggcc atcggcgcgg tcgctgcact gggtatcttc
     4981 ccgcttggcc tgggccatac gctttataat gcggcggtgc ggcgtgtcca cgccacctac
     5041 gccaacctga ttgccacgca ggaagtgacc ggcggcatca tcctgggtat cttcctgctg
     5101 ggcgagatac cgtcgcttgt gacggtgatc ggagtgattg tggcgctggt gggaatagcg
     5161 ggtgtgctgg tgtaatcaca actatagtgg atcaacaatc cacccctcat ctttgtcaca
     5221 ttccttcgat atcccatatc ccctccctcg ttcaaggtat gcccaccctg cttgcagcgc
     5281 gcacaatacc gcgtccagcc tgtctcccat cgcatcctgc gccagcgatt ctgccacatc
     5341 atcggacatt tccactgcca gcccataggt atccataagc agagcggagc gcagtccatg
     5401 tactatctcg tgccgcgcct gcacctgctc gcgggtttgc ttgccgcgct catcgctttt
     5461 gtagctgcgc ttgccgatca agcggcgcgc gaccaggacg ggatagcctt caattgctac
     5521 gcggttgtcg ctggttggac ggcagggcag cacactgacg cttgaacgga agaggcgggt
     5581 cgcgcccccg aaaaacatct tgccgacggg gacgcggtgc agcatcatgg gactgcgcgc
     5641 gctcgccagg atatcggtgg cgcgcaggag cagtttagcg ccgggcggtt ggctgttcat
     5701 gaagccttgc agcattgcca caaaggtagt cttgtccata gaggccaaga atttcatata
     5761 gtcgcaccag attgtaggcc agccgagttg ctcgatcagc gcgcgcggct ggccgaaagg
     5821 gaaatcgagg gcggccagcc aggggccatc ggcgcgcaga aaggcgtcga agtcgtcgag
     5881 cgtcgcgagt gaccggcatt cgtcgaggtg aagagtggtt cctttgattg tgcaggttgc
     5941 gcaggtgata ggctttcttg cagaaggagt agaagtgaag tcgaggccga agatgcggta
     6001 gtcgtacatg gtggtttcgc cgtcgcgcta gagctgcgcc atgactcgtt tgctgatgga
     6061 gcgcagggtg gcatagactc ctgtgcctga cgaggcgatg gcctcgaagc tggggacgcg
     6121 ccgccggttg aggtagcgtt ccagcacgtt tgttggcagg gccgagggca tatcgcgctt
     6181 attccattgc acgacgaagg gaaatgtagc gacatccttg ccgaggtggc gcaggttcat
     6241 ttccagttcg gaaatactct gtatattttc ttcaaacttt tccgcctgcg aatcgaccac
     6301 gaagacgatg ccgtctgcgc cgttcagcac ggccatgcgc gtgcgctcgt agagaatctg
     6361 tcccggcacg gtatagaggt ggaagcgggt gcgaaatccg tgtaccgtgc ctaaatccag
     6421 ggggaggaag tcaaagaaga gtgtgcgttc cgtctcggtg gcgatgctga gcatttcgcc
     6481 gcgtaccgat ggattgatgc ttttgaacac gtattgcaga ttggtagttt tcccacaata
     6541 accaatgcca tagtaaacaa tcttgcagtg tatttcgtgc gtcgcaatat tgatgagcgc
     6601 catgtagcgg gtacccctaa tcgcggaata gcagatcgat ggtatcttcg accgacgagc
     6661 ggaattggat attgaggaca gaagctttct ggcgtgtggt atcggccttg actcgttcga
     6721 gggtatgtgc cagttcgtca gaagctttct tggacagcac tttgacaagg ccgatgtggg
     6781 tcaatttatc gaacacgatc acgagcagcc attgatcttc gatcatggtg gtaaaaatat
     6841 tctgatggac gccttgctgg aatattgagc gaaaatcctt ttcgcccaac agttcggcaa
     6901 tttgccgaga ggaggagaag gccccggcca gcagcgcgcc aagcgacgtg gcgttgcgcc
     6961 tggtcgtcgc gccctgagtt acgatcacct ggccgctctt gtcgatgagc atggccgaag
     7021 aggcgctggt atcattcatg agttttgaga gggcattgga aataaagaca atttcctgat
     7081 ctgagatgat aaggttcgtc agttgttcca tcgatgcatt gcctctgtgt gtttcataag
     7141 cagtataaac gttgataata gccgccttgc tggtggctga acgctcagtc aaaagttgga
     7201 cagaatacga ttatttcata tcgagtgcgt caaggatatc agtgcgagtc gttttgacca
     7261 tgtgcctgac acggccaata ctggcggcgt tgtcgaaaag cgtcaccatc acggcaaaat
     7321 cgcccatcgg gtcaacgctg acgagaccgc gttcatattc attcaagacg ccgaccacgc
     7381 tgccgcgcgt aatctcctgg ctgagcgcct cagcggagtt cagcccgctc gtcataattg
     7441 cgccgatagc ctccaaatct tctttcccct cgcgtgccaa tccttcgatc atcaggccat
     7501 cgcgcccgac tagcacagcc agccgcactc ctgggatcgt caggaaacgg ctcaagcttt
     7561 gtttcaattc aaccatacat tcctccgtca ctgcgggcaa cgtcgcttca ggcacaaatg
     7621 tggcgtatca ctatcaatct ccataagctt ccccctattc tactttggta cctgtttgct
     7681 atgcaataga atgggacatc ttggctaatg gttttgtgac gcggacgtga taacgaccta
     7741 ttgacaagcg tcgaattcat caggtatact ttccgtaatc gtttacgata acgttatcgt
     7801 aaacgattac ggaatatttt ctggcagtgg gatggcccat tgaggggtag ctatgaccaa
     7861 gaagctgacc atccgcgata ttgcgggcct ggctgatgtt tctaccgcaa cggtctcgcg
     7921 cgtcctgaat cagaagcccg atgtagaccc ggcgactcgt gagcgcattc tgcgcatcat
     7981 ggaagagcag agctttatgc ccaacatcgc cgcttccggc ctggctggca gacgtagtca
     8041 cctgctcggc gtcctgaccc cttcgttcac ctggcctctc attccagaac tgatgcgggg
     8101 cattagcgaa gtcgtagaca gtacctcgta tgaactgatc ctgtatagca tcactgacgc
     8161 gaacgataag gatcccagtg atgtgattga ccgcatcgtg cgaacccggc tggcggctgg
     8221 cctgcttgct gtttttccgg gtctctcgac gcggcacctg accgaactga actcgcgcaa
     8281 cttccctgtt atcttgatcg acgatcaggg aataccgccc gaagggacgc cctggatcag
     8341 cgtcgaaaat cgaccaggcg cctatgaagc cacgcggcac ctgattcgcc tgggacatcg
     8401 ccgcatcgcg catatccagg gtccgctcag gtaccaggta tcgcatgacc gctacatggg
     8461 ctactgcgat gcgttgaacg aggccggtat tgcgcctgat cctatcctgg tgcgcgaggg
     8521 ggattttttg ccccgtggcg gctatatctc ctccaacgtc ttttttgatc tgcccgccga
     8581 gcagcgtccc accgccattt tcgccgctag cgattatatg gcctatggcg ctatttcagc
     8641 ggcggagcag cgcggactga gtgtgcctga ggatatggct gtagttggct tcgacgacaa
     8701 ccccacatct gcccatatgg aaccggcgct gacaaccgtt cgccagccgt tctacgagct
     8761 gggccgacgc gccaccgaaa tgctcctggc gctcgttgag acgccccgtt atgtgaatgg
     8821 cctgaatcac aatgggcgcg tcctgggcgc gccgtcgtca acggcctctg aatcgattcg
     8881 cattaagttg ccaaccagcc tgattgtgcg tacatcttgt ggcgcttctc gtggcgtcac
     8941 aatggtataa aaaagggggt gagcgccggc cataacaacg ttctgcaact ctctcttact
     9001 ctgatgggac attcgcggat tactgcgtag tgtcagcgat gtcggtgttg tgggtttcaa
     9061 agcgcaacgg tttgcgcagt gcgtaaaaaa cgtgttgcac cgttgtcagg ttgtcctttt
     9121 gtccttttta gcacattcga gaagcgagag acttcttttt gtgcgtggat catcgaatga
     9181 ttcgttttcc agcgttagga ggtaaaacgt gactcccaat ctcgcatctt caccacgtcg
     9241 gaaccggcgg gctacagtct tcttctcgct gttgcttccg ctggtaatgc tgctggcggc
     9301 ctgtggtggc actaccccaa ccactaccac aagcaaccat gcaacaacgc tgcgcgtgca
     9361 atgggctccc gggcaaccgg ttcccgatct ttttaacccg ttcttcaacg ccaaccaggg
     9421 cggcgcctgg ggcgcgcaag ggttgttgta tgatgaactc tacttcacaa acctgtataa
     9481 tggccagcat accccctggc tagccaacag ctaccagtac aacagcgacc tgacgcagtt
     9541 gacgttcacc atccgcccgg gagtgaaatg gaatgatggt cagcctttca caagcgcgga
     9601 tgtcttgttc acctttaacc tgatgcaggc gcaccctgca cttgaccaga actcgatctg
     9661 ggcaagtacg ctcaagagct tagctgcgcc cgatcccaat acagttgtgt tcacattgca
     9721 ggctcctgac agcacggcgc tcttttatat tgggggcacc ttcatcgtgc cgcagcatgt
     9781 ctcggggtgg acgacgggcg acccggccaa gttcgcgaat gacaagaatc cggtcggcac
     9841 cggcccctac ctgctctcga gcttcaatag cgatcagatt acctacacga gaaaccccaa
     9901 tttctggggc aatagtgtag ggatgacacc gcaggttcaa acgatccagg ctgttgcaac
     9961 caaggacaac acgacaacca tcaccaatat gatccagggt cagtacgact ggaccggtgt
    10021 cggttggagt cccgactttg attcccagta taaggcgaaa gacccgcagc acaaccagga
    10081 ctggtttgcc gcgagcaaca ccgttatgct ctacttgaac ctgcaaaagg cgccattcaa
    10141 caacctgctg gttcgtaaag cgatcagcgc ggcgattaac cgcgatcaac tgccgcaggg
    10201 cgtggcgctc tatgccaagg ttgccaatcc aaccggggtt atcataccca acctgaatag
    10261 ctggattgcg tcgcaatatc agagcgcggg tttccagtac agtgcgagcc aggctggcag
    10321 ttacctgcaa caggcc
//
LOCUS       DASZAK010000392         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_186222, whole genome shotgun sequence.
ACCESSION   DASZAK010000392 DASZAK010000000
VERSION     DASZAK010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186222"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /gene="groEL"
                     /locus_tag="VGT44_06900"
     CDS             <1..1033
                     /gene="groEL"
                     /locus_tag="VGT44_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV2580566.1"
                     /translation="SAYFVTNAEKMEASIDEPYILITDKKISTVADILPILEKLTQQG
                     RREVVIIAEDIDGEALPTLVVNKLRGILNVLAVKAPGFGDRRKEMLRDIAVLTGGQVI
                     SEEMGRRLDSVTLADLGQARRVVSHKDDTTIVEGRGDAADIQARIRQIKAQIEETTSD
                     YDREKLQERQAKLSGGVALIKVGAGSEVELKYRKTRVEDALSATRAGVEEGMVPGGGV
                     ALLNAIASLDSLKLTGDAATGVTILRRALEEPLRQLAINGGKDGSVVVEGVRRAQKEQ
                     KNDKIGYNVLADKYEDMVSAGIIDPAKVTRSALQNATSIAAMILTTEALVTDLPEKEK
                     PAAPAMPEY"
     gene            complement(1111..2076)
                     /locus_tag="VGT44_06905"
     CDS             complement(1111..2076)
                     /locus_tag="VGT44_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2580567.1"
                     /translation="MFCPRCHTELPDAAYACSQCGTVISPSAQASSSSIFYQPQQSSF
                     SYLPSGTPQWPSVVPPIGQMPKGMDAPDQGWSDKPSLAGTKPSTRRGSLGIPAMIAIF
                     LVCVVVGGGLTFGLLKLEGANNQSPAPVILHINTTPAATSTPGGAQVSPTATTTNQLP
                     TPTSFLILSNNDVGLSFKYPSTWAIDPPQLTTQSAVIDTHPSTQNGLLMNLERFSTSG
                     SANFKTTGDVNNNNITQIQNISGLTNFQNVQPTSPQQAIGGVQWDEQDITFSNSNGVV
                     FYFVTIAVKYKNQYYDIFYSAPQNVFSEAVQKYYQPMLSSLKFLS"
     gene            complement(2110..2319)
                     /locus_tag="VGT44_06910"
     CDS             complement(2110..2319)
                     /locus_tag="VGT44_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580568.1"
                     /translation="MPIIFEGNEHRYVRCVIADAGDPERRFECRVLDVDDVPISIGEP
                     ITLEVVKVITERRGGIVRFYCRLVM"
     gene            complement(2456..>3027)
                     /locus_tag="VGT44_06915"
     CDS             complement(2456..>3027)
                     /locus_tag="VGT44_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2580569.1"
                     /translation="GPLLFCGAEVHHSTLGLIGAGRIGKIMAKRASGFEMRVIYYDVF
                     RLSPEDEAAYHMTYLPMDEVLQQADFVSVHTPYMPSTHHLIGERELKLMKPTAILINT
                     ARGPIVDEKALAVALQSGIIAGAGLDVFENEPVVEPGLLTLEQAVLLPHIASASLKTR
                     TLMATMASDNLVAHFHGQQPPNILNPEVL"
BASE COUNT          660 a          801 c          913 g          653 t
ORIGIN      
        1 gtctgcctac ttcgtcacca atgctgagaa gatggaagct tcaatcgacg agccctacat
       61 tctcatcacc gacaaaaaga tcagcacggt tgccgatatt ctgccgatcc tggagaagct
      121 gacgcagcag ggccgccgcg aagtggtgat tattgccgag gatatcgatg gcgaagcgct
      181 gccgaccctg gtcgtcaaca aactgcgcgg tatcctcaat gtgctggcgg tgaaggctcc
      241 tggttttggc gatcgccgca aagagatgct gcgcgatatc gctgtgctga ccggtggcca
      301 ggttatcagc gaagagatgg gccgtcgcct cgatagcgtc acactcgccg atctcggcca
      361 ggctcgccgc gtggtttccc acaaggatga caccaccatc gtcgagggcc ggggcgatgc
      421 cgccgacatc caggctcgca tccgccagat caaggctcag attgaagaga ctaccagcga
      481 ttacgaccgt gagaagttgc aggaacgcca ggccaagctg tccggaggcg tagcgctcat
      541 caaggtcggg gctggctccg aggttgagct gaaatatcgc aagacccgcg tggaagacgc
      601 gctctcggcg acccgcgccg gtgtcgagga gggcatggtt cctggcggcg gtgtcgccct
      661 gctgaatgcc atcgcttctc ttgactcgct taagttgacg ggcgatgccg cgacgggcgt
      721 cacgatcctg cgccgcgccc tcgaagagcc actgcgccag ctcgctatca acggtggcaa
      781 ggacggctcg gttgtcgtcg aaggtgtgcg ccgcgctcag aaagagcaaa agaacgacaa
      841 gatcggctac aatgtgctgg ccgataagta cgaggatatg gtctctgccg gtatcatcga
      901 tcccgcgaaa gtgactcgct ctgccctgca gaacgccacc agcattgccg cgatgatcct
      961 gacgactgag gcgctcgtca ccgatttgcc cgaaaaagag aagccagccg ctccggccat
     1021 gccagagtac taggataaca ggcgtacccc aaaaaacaga tccccctctc cgcaagagag
     1081 agggggatct gttttttggc acgccgcctg ctacgacagg aatttgagcg aactcagcat
     1141 cggctggtag tacttctgga cagcctcgct gaagacgttt tggggcgccg aatagaagat
     1201 gtcgtaatac tggtttttgt acttgacggc gatagtgacg aagtagaaga caacgccgtt
     1261 cgagttgcta aatgtgatgt cctgttcatc ccactggaca ccgcctatcg cctgctgcgg
     1321 tgatgtcggc tgcacgttct gaaaattcgt caggcccgaa atgttctgaa tctgggtaat
     1381 gttgttgttg ttcacgtcac cggtcgtctt aaagtttgcc gatccgctgg ttgagaagcg
     1441 ttccaggttc atcagaaggc cattctgcgt ggatgggtga gtgtcgatga cggcgctttg
     1501 tgtggtcaat tgaggtggat caatcgccca ggtcgaggga tatttgaaag aaaggcccac
     1561 gtcgttattg ctcagaatga ggaaggatgt cggcgttggc aattgattgg tggttgtggc
     1621 cgtcgggctg acctgtgccc ctccgggtgt cgatgtcgcc gcgggagtgg tattgatgtg
     1681 cagtataacg ggcgctggcg actgattatt ggcgccttcc agcttcagca agccaaaggt
     1741 caggccgccg ccaactacga cgcagacgag aaaaatggcg atcatggctg gaatacccag
     1801 gctcccacgg cgggtagatg gtttggtgcc tgcgagtgat ggcttgtctg accatccctg
     1861 gtccggtgcg tccatccctt taggcatctg tcctatgggt gggacaacgc tgggccattg
     1921 cggcgtgccg ctgggcaagt aggaaaacga tgattgttgt ggttgataga atattgatga
     1981 cgaggatgcc tgggcggagg gtgatatgac cgtgccgcat tgcgaacagg cataggctgc
     2041 gtcaggcagt tctgtgtgac aacgagggca gaacataggt ggtttatcct ttatgctaca
     2101 tgtgagtgtt cacattacca gacgacaata gaaacgtaca atgccaccgc gccgctccgt
     2161 gataactttt accacttcca gcgtaatcgg ttctccaata gagatgggaa catcatctac
     2221 atctaataca cgacattcga aacgacgttc ggggtcgcca gcatcggcga tgacgcagcg
     2281 cacgtaacga tgttcgttgc cctcaaatat aataggcaat cctggctcct tctctgccag
     2341 gatgccagag cgtatctgtg ccataaattg atatgcgctc ccatcagaat gttcttacaa
     2401 agaactataa agaagggaag gcaaattgtc aactacctac aggccagggc ggttgctaca
     2461 gaacctccgg gttgagtata ttgggtggct gctgcccatg aaaatgagca acgaggttat
     2521 cgctggccat ggttgccatc agtgtgcgcg tcttgagcga tgcgctagcg atgtgcggca
     2581 gcagcacggc ttgctccagt gtgagcagcc ctggttcgac gactggttcg ttctcgaaga
     2641 catccaggcc cgcgcccgcg atgatgccag attgtaacgc gaccgcgagc gccttttcgt
     2701 ccacaattgg cccgcgcgct gtgttgatca ggatggcggt gggcttcatc agcttgagtt
     2761 cgcgctcgcc gatcaggtga tgggtggaag gcatgtaggg cgtgtgaacg ctgacgaaat
     2821 cggcctgctg caatacctca tccatgggca ggtaggtcat gtggtaggca gcctcatcct
     2881 ctggcgacag gcgaaaaacg tcgtagtaaa tgacgcgcat ctcgaagcca ctggcgcgtt
     2941 tcgccatgat tttgccgatg cgccctgctc caatcaggcc cagagtggaa tgatgcacct
     3001 cggccccgca gaagagcagc ggccccc
//
LOCUS       DASZAK010000393         1940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_44368, whole genome shotgun sequence.
ACCESSION   DASZAK010000393 DASZAK010000000
VERSION     DASZAK010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44368"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VGT44_06920"
     CDS             <1..522
                     /locus_tag="VGT44_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580570.1"
                     /translation="LVTAAAILLFVLLGPGLIFELNLAGNPGARINGGASTSLVSDLP
                     SDLSFAILSTKDIPRGLTLLLPTNLAPNGYLVATTMNNYGSSVPVNLYLVYTQNAHLY
                     ESPSPPLPTEAYANSSYSTIQLGSSSGILLHTSNGENRIEWYQSGLLCDMVSAEPVQL
                     MATMIEQLQTVTY"
     gene            complement(642..>1940)
                     /locus_tag="VGT44_06925"
     CDS             complement(642..>1940)
                     /locus_tag="VGT44_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580571.1"
                     /translation="STRWLGWFNMPPDIQEMRSYIDAGLQLLADKPQSGEHAAFLTYL
                     AMWSIRQVKHASPDERPAIAEAALANIHEALRIAEEVNDTAALWVTLDALGFIYEHQH
                     LYLDAHKAHHRRQELASLIKGREELYDLYMSLGWSHLHIADYAAAAMWFGRAWRIAQT
                     MESPSMLLLSMLGRMYVWYEWNRWSEVRVVAYDAMQMAEQYQLDDQWLLDSLETLADI
                     AYRTGALEESDSLVRQYRRMAEQRGIEPELTRSIYLAREEWARAYSDFKEALQRSEPF
                     PRPFVVAIVAELAVTLGEDIETQQQLCERAVTLTERSGARKYQVIALRARGRMSLGQA
                     RYQDAEHDLKQALALCEMLDLPWEQGQTLTSLGFLYRERAKTAASGSEDAQAADASLA
                     HHYFERALGYFESLKAIHDAERVRLALAEDAEVIRQDTLP"
BASE COUNT          367 a          630 c          540 g          403 t
ORIGIN      
        1 ctggttactg ccgccgctat cctgctcttc gtgctgctgg ggccgggcct catcttcgag
       61 ttgaatctgg ccggaaaccc gggcgcgcgc atcaatggag gcgcgtcgac cagcctggtt
      121 tctgacctcc catccgatct ctccttcgcc atccttagca cgaaagatat cccgcgcgga
      181 ttgacattgc tcctgccgac aaatctcgcg cccaatggct acctagttgc cacgaccatg
      241 aacaactacg gcagcagcgt ccccgttaat ctttacctgg tctacaccca gaacgcgcat
      301 ctctacgagt caccttcgcc gccgctaccc accgaagcct atgcaaactc gtcctattct
      361 actatccagc ttggctcatc cagcggcatc ctgctgcaca ccagcaacgg cgagaatcgc
      421 atagaatggt accaatccgg ccttctatgc gacatggtca gcgccgagcc ggtacagctc
      481 atggcaacca tgatcgaaca attgcaaact gtgacgtatt gagcaccctc gccatcgccc
      541 cacccgcctg ctgtcatcct gagcgcagcg acggatctcc cgctgggtca ggcgcagttc
      601 cttcgctgcg ctcaggatga cagcagggct tacgtgtacg cttatgggag cgtatcctgg
      661 cggatcacct cggcatcttc cgccagcgca agtcgtacac gctcggcatc atggatggct
      721 ttgagcgact cgaagtagcc gagcgctcgt tcaaagtagt ggtgtgcgag gctcgcatca
      781 gccgcctgag catcctcact gccggaagca gcagtctttg ctcgctcgcg gtacagaaat
      841 ccgaggctgg tgagcgtctg accctgctcc cagggcaggt cgagcatctc gcacaaggcc
      901 agcgcctgtt tgagatcgtg ttcagcatcc tggtagcgcg cctgacccag gctcatacgt
      961 cccctggcgc gcagcgcgat tacctggtat ttgcgcgccc ccgaccgctc ggtcagcgtc
     1021 accgcgcgct cgcacaattg ctgctgcgtt tctatatcct ctccaagcgt aacggccagt
     1081 tccgcgacaa ttgccaccac gaacggcctg gggaacggct cggaacgctg caaggcttcc
     1141 ttgaaatcgc tgtaggcgcg cgcccactct tcacgcgcca ggtagatgct gcgcgtcagt
     1201 tccggttcga tgccgcgctg ttcggccata cggcgatact ggcgcaccag cgaatcgctc
     1261 tcttcgagcg cgccggtgcg gtaggcgatg tcggccagcg tctccagcga gtccaatagc
     1321 cactggtcgt caagctgata ctgttcggcc atctgcatcg cgtcataagc caccacgcgc
     1381 acctcgctcc aacggttcca ttcgtaccag acgtacatgc ggccaagcat actcaacagt
     1441 agcatcgaag ggctttccat ggtctgcgcg atgcgccatg cccggccaaa ccacatggcg
     1501 gcggcggcat aatcggcaat gtgtaaatgc gaccagccca gggacatgta caggtcgtag
     1561 agttcttcgc gccccttgat gagcgaggcc agttcctgcc gccggtgatg agccttgtgg
     1621 gcatccaggt aaagatgctg gtgttcgtag atgaatccga gtgcgtccag cgtcacccaa
     1681 agcgcggcag tgtcgttgac ctcctcagcg atgcgcagtg cctcgtggat gttcgccagg
     1741 gctgcctcag cgatggcggg tcgttcatcc ggactggcgt gtttgacctg gcgaatagac
     1801 cacatggcga gataggtcag gaaggccgcg tgttccccgc tttgtggctt gtccgctagc
     1861 agttgcagtc cggcatcgat ataggagcgc atctcctgta tatcgggcgg catattgaac
     1921 catcccagcc agcgcgtcga
//
LOCUS       DASZAK010000394         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_81253, whole genome shotgun sequence.
ACCESSION   DASZAK010000394 DASZAK010000000
VERSION     DASZAK010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81253"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1508
                     /locus_tag="VGT44_06930"
     CDS             213..1508
                     /locus_tag="VGT44_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV2580572.1"
                     /translation="MQTHNGELARASSRNAARHAGTQPAEEGIASSGIAFKLWRLYQH
                     AWLVCLLFPLVQLLRNPLPVWHLILGGVALVGFAASYTWLMWPHPASRGARLRARSRS
                     SFLLFVMLSVLVLVFSVLDNPTWLWLLIGVSAIAGLLLPLRSAFAAVVLFTLFPLFLM
                     LKIHEGLTGVDLWWLIALMLLVRGLGLDMIGVARMGSAIRELQSARRELARLAVVEER
                     ERLSRDLHDLLGQTLSMITLKSELARQLVKEDPERCAQELTEIERVSRQTLREVRKTV
                     TGYRQPTLTNELDGARQLLEAARVECVVEQTTGDLPPDLNTILAWTVREGVTNAIRHS
                     RTQRCLIRLTRNQGTVEAVILNDMERQEDQRDKRTGQGLGLSGLRERVAALEGTMEAG
                     PLVLSGKSYFRLSVAFPMQNCGGAGKGGGVTTGNQGEDA"
     gene            1505..>2008
                     /locus_tag="VGT44_06935"
     CDS             1505..>2008
                     /locus_tag="VGT44_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580573.1"
                     /translation="MIRVLLAEDQAMVRGALAALLSREKDIEVVAEVARGDGVVEAAS
                     ASQPDIALLDIEMPGLSGLDAAQALRRALPSCRVVILTTFGRSGYLRRAMESGAVGFL
                     LKDAPAAELARAMRRVMQGERVVDPELALLALSEGSNPLTPREREVLSAALSGIGHAE
                     IAARLSVS"
BASE COUNT          385 a          562 c          659 g          402 t
ORIGIN      
        1 cccgcgagat gtcgtggtgc tggcttgctc ggcccttctg gcggtaggtc tgggtatcct
       61 ggcgatgagc cggggcatgg ccgagttccg gtaaggaagg caagagcgaa cgaagaggtc
      121 gatttattgc tgagaaagac catctagaca aagagatggt agaatacttg cgtgagaaaa
      181 gcctgttgca gcagaaaggg ggttcttctc ctatgcagac acacaacgga gagctggcgc
      241 gagcgtcgtc ccgtaatgcc gctcgccatg ctggcacaca gccagcagaa gagggcattg
      301 cttccagcgg catcgccttc aagctctggc gactgtacca gcacgcctgg cttgtgtgcc
      361 tcctcttccc cctggtccag ctattgcgca atcccctccc ggtgtggcat ctcatcctgg
      421 gaggagtggc cctggtcggg ttcgcggcca gctatacctg gctcatgtgg ccacatccgg
      481 cgagtcgggg agcgcgcctg cgggcgcgtt ctcgcagttc attcctcctc tttgtcatgc
      541 tttccgtgct ggtcctcgtc ttcagcgtac tggataatcc tacctggctc tggctcctga
      601 ttggggtgag cgccattgct ggactcctcc tgcccctgcg aagcgctttt gctgcggtgg
      661 tactctttac cctcttcccg ctcttcctga tgctgaagat acacgagggt ctgacagggg
      721 tcgatttgtg gtggctcatc gcgctgatgc tgctggtgcg cgggcttggc ctcgacatga
      781 tcggtgtggc gcgtatgggc agcgctattc gggaactgca gagcgcgcgt cgagaactgg
      841 ctcgactggc ggtggtcgag gagcgcgagc gcctgtcgcg cgatctgcac gacctgttgg
      901 ggcagaccct ctccatgatt acgctcaaaa gcgaactggc acggcaactg gtcaaggaag
      961 accccgagcg ctgcgcccag gaacttacgg agatcgaacg cgtatcgcgc cagacgctgc
     1021 gagaggtgcg caaaaccgtc acgggttatc ggcagccaac cctcacgaac gaactggatg
     1081 gggcacggca actgctcgaa gcggcaagag tggagtgtgt ggtcgagcag acgaccgggg
     1141 accttccgcc agacctcaat accatcctgg cctggaccgt tcgagaaggc gtgaccaacg
     1201 cgatccgcca tagtcgcacg caacggtgtc ttatccggct caccaggaac cagggaacgg
     1261 tcgaggcggt gatactcaac gatatggagc ggcaagagga tcaacgggac aaacgcacgg
     1321 gccagggcct cggcctttct ggcctccgcg aacgcgtagc ggcgctggaa ggaacgatgg
     1381 aagctggtcc gcttgtctta tctggcaaat cgtactttcg tttatcggta gcgttcccta
     1441 tgcagaactg cggaggagca ggaaagggtg gaggagtgac aacgggaaac cagggggaag
     1501 atgcatgatt cgcgtgttgt tagccgaaga ccaggccatg gtccgcggag cgcttgcagc
     1561 gctgctcagt cgagaaaagg acattgaggt cgtcgcagaa gtcgcacgag gagatggagt
     1621 ggtcgaagcg gcgagcgcct cgcagccgga catcgcgctg ctggatattg agatgcctgg
     1681 cctgagtggg ctcgatgctg cgcaggcatt gcgcagggcg ttgccctctt gccgcgtggt
     1741 aatcttaacg acctttgggc gcagcggcta tctacgacga gcaatggaga gcggcgcagt
     1801 tggatttctg ctcaaagatg cgccggctgc agaactggcg agagcgatga ggcgtgtgat
     1861 gcaaggagaa cgggtcgtcg atccagaact ggccttgtta gctctctccg agggcagcaa
     1921 cccgctgacg cctcgcgagc gcgaggtgct ttccgctgcc ctttcgggga tcggacatgc
     1981 cgagatcgct gcacgccttt ctgtctcc
//
LOCUS       DASZAK010000395         2661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_649394, whole genome shotgun sequence.
ACCESSION   DASZAK010000395 DASZAK010000000
VERSION     DASZAK010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649394"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VGT44_06940"
     CDS             complement(<1..613)
                     /locus_tag="VGT44_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580574.1"
                     /translation="MEQDQTRATNPGHVPTGWNSDSDLDDAMLTDEDVSQQFVVPGNA
                     NSWDVSQDDDTSAFTNIWRQLSWIIVPIPFAIIIFLFAMLASSRGIANLAPLPLAIIL
                     LALVVIQGTFLYYAGNNDTLWTLSIVGGYLLFLLVGAFVFFGINGALIVGGAFLVLFV
                     LVSIVFGRRRAQRRKDKAEQDRFKDEAAAARRQAPAEAQRIAEE"
     gene            complement(663..1760)
                     /locus_tag="VGT44_06945"
     CDS             complement(663..1760)
                     /locus_tag="VGT44_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEV2580575.1"
                     /translation="MPPTVSASDVVVTIVGIRFRPAGRIYYFDPQGQAFATGQYVIVE
                     TVRGVEAGRVVIAPKTIPERELSDPLKPVLRLASEDELRMMLSFKGKEKDALAQCDRQ
                     VKQHKLPMKLVEAEYTFDGSRLTFYFIAEERVDFRALVRDLAATFRTRIELRQIGARD
                     QAKLQGGVGICGKTLCCSSWITDFGVVSIKMAKEQDLPLNPAKISGVCGRLLCCLAYE
                     NDTYARAKQTMPPVGSFMDTPSGSGKVVSINVPRSSVEVMLESGVIIQVPVSDSAAGG
                     CGSCSKAGACGIQAAKMAMPASSETRSGCGNPNCTGACSSGGACTCGTGAKTDTENTS
                     AVSTAAGRGSGACGIKRNATPRTLDETYRSR"
     gene            complement(1783..2355)
                     /locus_tag="VGT44_06950"
     CDS             complement(1783..2355)
                     /locus_tag="VGT44_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2580576.1"
                     /translation="MPTRILIADDETIQRMDLKDILTKQGYLVVGEAGDGSSAVNLAR
                     EVRPDLVIMDIRMPDMDGIAAAEALTQEKIAPVLLLTAFGDQPLVERAKEAGVVNYIV
                     KPLRESEVSPAIEVALARYNEFRAMEEKARTLTDQLETRKVVERAKGLLMEKQGLSEQ
                     EAFRKIQKASMNNRKSMREVAEAILLTNEM"
     gene            complement(2451..>2661)
                     /locus_tag="VGT44_06955"
     CDS             complement(2451..>2661)
                     /locus_tag="VGT44_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580577.1"
                     /translation="TVKKERYMDSIEELEMLRNNGLAEEEISRLRRLRRDYKTRTRQR
                     TLAELRRLEFARWLVTSGRLTEHIA"
BASE COUNT          549 a          772 c          738 g          602 t
ORIGIN      
        1 actcctcggc gatgcgctga gcttctgccg gggcctgtcg gcgcgcggca gcagcctcat
       61 ccttgaagcg atcctgttct gccttgtctt ttcgacgctg cgcgcgccgt cgcccaaaga
      121 cgatactgac cagcacaaac aacacgagga aagccccgcc gacgatcagc gcgccattga
      181 tgccgaagaa gacgaatgcg ccgaccagca agaagaggag gtagccaccg acgatggaga
      241 gcgtccacag ggtgtcgtta tttcccgcgt aatagaggaa ggttccctgt atgacgacta
      301 gggcgagcaa aataatggcg agcggcagag gcgccaggtt agcaatgccg cgcgaactgg
      361 cgagcatcgc aaaaaggaaa ataatgatag cgaatgggat cggcacaata atccacgaga
      421 gttgacgcca gatattggtg aaggcgctgg tgtcgtcgtc ctgggagaca tcccagctat
      481 tggcgttgcc cggtacgaca aattgctgcg agacatcctc atccgtgagc atggcgtcgt
      541 ccaggtcgga atcggaattc cagcccgtgg gaacgtgtcc agggttggtg gcgcgtgttt
      601 gatcttgttc caatgtaaat gattctcccc tctatcttga atgtctaaac gatcgctcgc
      661 tgttagcgcg aacggtaggt ttcatccagt gtgcgaggag tcgcatttcg ttttataccg
      721 caagcgccac ttcctctacc ggctgctgtg ctgactgcgg aggtgttttc cgtgtccgtt
      781 ttcgcgcctg tcccacaggt acacgccccg cccgaactgc aagcgccagt acaattcgga
      841 ttgccgcatc cactgcgcgt ttcactactc gccggcatgg ccatctttgc cgcctggatg
      901 ccgcaggcac ccgccttgct gcatgagccg cagccaccgg ctgccgaatc ggatacaggt
      961 acctggataa tcacgccgct ttcgagcatt acttcgacgg atgagcgcgg cacattgatc
     1021 gagacgacct tgcctgagcc gctgggggtg tccatgaaag agccaacggg cggcatggtc
     1081 tgtttggccc gtgcgtacgt atcgttttca taggccaggc aacagagcag acgaccgcaa
     1141 acacccgaaa ttttggcggg attgagcggt aaatcctgct ctttcgccat tttgatggag
     1201 acgacgccga aatcggtgat ccaggaacta caacacaggg ttttgccaca gatgccgacg
     1261 ccgccctgca acttggcctg gtccctggca ccaatctggc gcagttcgat gcgtgtgcga
     1321 aaggttgcgg cgagatcgcg aacgagcgcg cgaaaatcga cgcgctcttc ggcgatgaaa
     1381 tagaaggtca gccggctgcc atcgaatgtg tattccgcct ccactaattt catgggcagc
     1441 ttgtgctgct tcacctgccg gtcgcactgc gccagggcat ctttctcttt gcctttgaag
     1501 gagagcatca tgcgcagctc atcttcactg gcaagacgca aaacgggctt gagcgggtcg
     1561 cttaactccc gttcaggaat ggttttgggc gcgatcacca cgcgccccgc ttctacgccg
     1621 cgaaccgttt cgacaattac gtactggccg gtcgcaaacg cctgcccctg cgggtcaaag
     1681 tagtagatgc gcccggcagg acgaaaacga ataccaacta tagtcaccac gacatcgctg
     1741 gcgctcacag ttggaggcat agatgctcca atcctagaca cactacattt cgttggtcag
     1801 cagaatggct tccgcgactt cgcgcatgga tttgcggtta ttcatgctgg ccttttgaat
     1861 cttgcggaag gcttcctgtt cggaaagtcc ctgcttctcc atcagcagcc ccttggcgcg
     1921 ctcgacgacc ttgcgcgttt caagctgatc ggtcagggtc ctggccttct cttccatggc
     1981 gcggaactca ttgtagcgag cgagcgcgac ttcaatggcg gggctgactt cactttcacg
     2041 caggggcttg acgatgtagt tgacgactcc cgcttctttg gctcgctcta caagcggctg
     2101 gtcgccgaag gcggtcagga gcagcacggg ggcgatcttt tcctgggtga gcgcctctgc
     2161 tgctgcaatg ccgtccatat cgggcatacg aatatccata ataacaaggt ccgggcgtac
     2221 ctcgcgggcc aggttgacgg cgctcgatcc gtcgccagcc tcacctacta ccagatagcc
     2281 ctgcttggtc aggatgtctt tcaaatccat gcgctgaata gtttcgtcat cagcaatcag
     2341 aatgcgtgtg ggcatggcag ttcctcgtct tgaatgtgca tcgttcaccc agcaagcaga
     2401 aacacgtagc ttattgacgg gcttagcaac ctgcgaacag ccgcaccccc tcaagcgatg
     2461 tgctctgtaa gcctgccaga agttacaagc cagcgtgcaa actcgagacg tcgaagttcc
     2521 gcaagggttc gctgtctcgt tcttgtcttg taatctctgc gtagcctcct caatcggctg
     2581 atctcttcct ctgccaagcc gttgttacgc aacatttcga gttcttctat gctgtccata
     2641 tatctctcct ttttcacggt g
//
LOCUS       DASZAK010000396         4015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_186336, whole genome shotgun sequence.
ACCESSION   DASZAK010000396 DASZAK010000000
VERSION     DASZAK010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186336"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VGT44_06960"
     CDS             <1..562
                     /locus_tag="VGT44_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S53"
                     /protein_id="HEV2580578.1"
                     /translation="TTSFPTDDPWVTSTGGTTLDRTQNGFAETAWNNSLGASGGGFSA
                     FFSTPSYQQSLPGAVQQLLNKRRGVPDVAADADPNTGLAIYVNGWTMAGGTSASAPFW
                     AAITAIANQVAGQPLGFLNVGLYKLGASSTYARDFRDITSGNNTRVVNGIIVPGHNAV
                     AGWDPVTGLGSPDAENLIPDLIAALQ"
     gene            731..1993
                     /gene="hflX"
                     /locus_tag="VGT44_06965"
     CDS             731..1993
                     /gene="hflX"
                     /locus_tag="VGT44_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV2580579.1"
                     /translation="MVAVEHNESESLWSVEDSLSELGTLARTAGAEVVGTMIQRMRHP
                     DVATYIGKGRAQELCDTEKQLRVDLVIFDDELSPSQQRNLEKLLHSRVIDRTALILDI
                     FAQHARTREGRLQVELAQLEYWLPRLAGRGVELSRQAGGSRSAGATGAGGAIGVRGPG
                     ETKLEMDRRRTRERIAELRDEIEAVRDQRKLHRAQRAAQAIPVVAVVGYTNAGKSTLF
                     NTLTEAGVIAEDRLFATLDPTTRHILLPRNAEVLLTDTVGFIQKLPTRLVAAFRATLE
                     EVVDADLLLEVVDVSHENAIEQSETVSDILKSLSASDKPRVTALNKVDLLDDPTELDT
                     SLYPNAVPVSALHKTGLDALREKIAAVLAVSMEPVEVVIPFDRGELVELFHRRGHVER
                     EEHREEGTLLVGRLPRSLRGYFAQYARA"
     gene            2080..3024
                     /locus_tag="VGT44_06970"
     CDS             2080..3024
                     /locus_tag="VGT44_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2580580.1"
                     /translation="MTETARFRARFWGVRGSYPTPGPQTVRYGGNTSCVEVRAGTQTI
                     ILDAGSGIIRLGYDLLRLHADGSPIDVSLFITHGHGDHLVGLPFFNPMYEPRTYIRFF
                     GPRLSGRSIEELVTPLMSQPYFPVDIRKLPSHRAFHTLQGTECITWREGESEPLIEQP
                     TMRPMAGLSEGDVCLTVRLTNSHPLDGAAIYQISYGGRRLVYATDVEWKERCEPAFLD
                     FVRGADVLIHDAQYTPDDYFAAKQGFGHSSFQVATQVARDAQVGTLILFHHEPTYDDD
                     KLDALQAEAQAQFPRTYSACEGMEIDLLTDIPTVRTRG"
     gene            3027..3779
                     /locus_tag="VGT44_06975"
     CDS             3027..3779
                     /locus_tag="VGT44_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2580581.1"
                     /translation="MLIDVVLAFMIIVSFLIAMILHEIGHSLVAAWMGDPTPRTEGRM
                     SLSLAPHINPLGTLLCVILAFFPVGSSVGLGWGNPVKADPWKLRGGPNVGTLIVALGG
                     IFTSLLLGGIFAGVLRILPVELYSNAFIVRVPQLIAVFAIVNISLAIFNLMPLYPLDG
                     YQILYSLLPSRQAVSFAKSAPYGPFIILLLIFLLPFLGQLTGLSDFFLFRISFYILQG
                     SLQLVGLISGAGGAGACAVYLGLAPLVPLLCG"
     gene            3785..>4015
                     /locus_tag="VGT44_06980"
     CDS             3785..>4015
                     /locus_tag="VGT44_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580582.1"
                     /translation="MQAFTYRLWQVSQQLGFVAPLSEDERREVARWLPESAYALFTSM
                     SPADQRHALRVCQGLQARGCAEPDMLAAALLHD"
BASE COUNT          782 a         1188 c         1233 g          812 t
ORIGIN      
        1 aaccaccagc ttccccacgg atgatccctg ggtgacgagt acgggaggca cgacgctgga
       61 ccgcactcag aacggctttg cggaaacggc atggaacaat tctttgggcg cgagtggcgg
      121 cggattcagc gcgttctttt ccacgccgtc gtaccagcag agcctgcctg gagccgtgca
      181 gcaactgctg aataagcggc gcggcgtgcc agatgtcgcg gcggatgctg atccgaatac
      241 cggcctggcg atctacgtca atgggtggac gatggctggc ggcacaagcg ccagcgcgcc
      301 gttttgggcg gcaatcacgg ccatcgcgaa ccaggtggcc ggccagccgc tcggcttcct
      361 caatgttggg ctgtacaagc ttggggcatc ctcgacctac gcgcgcgatt tccgtgacat
      421 caccagtggg aacaacacca gagttgtgaa cggcatcatt gtgcctggcc acaacgccgt
      481 agctggctgg gaccctgtta ccggcctggg ttcgcccgat gccgaaaacc tgatacccga
      541 tttgatcgcg gcgttgcagt gatgacgaaa ttggccatac aataatcgat agaaacgagt
      601 tccgtgtagc atggtacaat aaagaaaaag caactcggta gcaaatctga tctagtggag
      661 ggaacttacg gccaggcaac gcttttatga gaccggcgct cgcgggcgag gcagacatga
      721 gtcggccttc ctggtcgcgg tagagcataa tgaaagtgaa agtttgtgga gcgtggaaga
      781 ttcgctcagc gagctaggga cactggcgcg cacggctggc gcggaggtgg tcggcacgat
      841 gatccagcgt atgcgtcatc ccgatgtcgc cacctacatc ggcaaagggc gcgcccagga
      901 gttatgcgat accgaaaagc agctgcgtgt tgaccttgtc atcttcgacg atgaactctc
      961 cccctcgcag cagcgcaacc tggaaaagct gctgcactcg cgcgtgatcg accgcacggc
     1021 cctgatcctc gacatcttcg cccagcacgc gcgcacacgc gaaggacgct tgcaggtaga
     1081 actggcgcag ctcgaatact ggctgccacg cctggccggg cgcggcgttg agttgtcgcg
     1141 gcaggcgggc ggctcgcgca gcgcgggtgc aaccggcgcg ggcggcgcaa tcggtgtgcg
     1201 cggcccaggc gaaacaaaac tggagatgga ccgccgccgc acacgcgagc gtatcgccga
     1261 actgcgcgac gagatcgagg ccgtgcgcga tcagcgcaag ctgcaccgcg cgcagcgcgc
     1321 agcgcaagct attcctgtgg tggccgttgt gggctacact aacgcgggca agtccacgct
     1381 tttcaacacc ctgacggagg ccggggtgat cgctgaagac aggcttttcg ccacgctcga
     1441 tccgaccacg cgccatattc tgttaccccg caacgccgaa gtcctgctga ccgataccgt
     1501 cggcttcatc cagaagctgc cgaccaggct ggttgccgcc ttccgcgcca cactcgaaga
     1561 ggttgtcgat gccgacctgc tgctcgaagt cgtcgatgtc agccacgaaa acgccatcga
     1621 gcagagcgag actgtcagcg atatcctcaa aagcctatcg gccagtgaca aaccgcgcgt
     1681 gaccgccctc aacaaggttg acctgctcga cgacccgacc gaactcgaca cctcactcta
     1741 tcccaacgcc gttccagtct ctgcccttca caaaaccggt ctggatgccc tgcgcgaaaa
     1801 aatagcggcg gtgctggccg tcagcatgga gccggtcgag gtggtgatcc cgttcgatag
     1861 aggcgaactg gtggaattgt tccataggag agggcatgtc gaaagggaag agcaccggga
     1921 ggaagggacg ctgctcgtag gacggttgcc gaggtcgctg cgagggtatt ttgcgcagta
     1981 tgcgcgggcg tgatgcttct atcgctgctt tgacatcaca cgatataatg tgaccaaccg
     2041 aacaaatcat cgttcaaagc ggcggtagga gcaatgcgca tgacagagac agcacggttc
     2101 cgcgcgcggt tctggggggt acgcggcagc tacccgacgc cggggccgca gacggtacgc
     2161 tatgggggca ataccagttg cgtcgaggtg cgggccggga cgcagaccat catacttgat
     2221 gccgggagcg gcatcatacg cctggggtat gacctgctgc ggcttcatgc ggacggctcg
     2281 cccatcgatg tctcgctgtt catcacgcat gggcatggcg atcaccttgt cgggctgcca
     2341 ttcttcaatc ccatgtatga gcctcgcacc tatatacgct tctttggccc gcgtctctca
     2401 gggcgtagta tcgaggaact tgtaacccca ctcatgtctc agccgtattt tccagtggat
     2461 atacggaaat tgccgtcaca tcgcgcgttt cataccttgc agggaacgga atgtatcacc
     2521 tggcgcgagg gcgaaagcga gccgctgatc gagcagccaa ccatgcggcc tatggccggt
     2581 ctatctgagg gcgatgtatg cctgacggtc aggctgacca acagccaccc gcttgatggc
     2641 gcggcgatct atcagattag ctatggcggt cggcggttgg tttacgccac ggacgtagag
     2701 tggaaagagc gctgcgaacc tgccttcctg gattttgtca ggggcgcaga cgtgctgatc
     2761 catgacgcgc agtatacgcc agatgactat ttcgcggcca aacagggctt tggtcacagt
     2821 agcttccagg tggcgacgca ggtcgcgcgc gatgcgcagg tgggcacgct gatcctgttt
     2881 catcacgagc cgacctatga cgacgacaag ctggatgcgc tgcaagccga ggcgcaggcg
     2941 caatttccgc gtacctattc cgcctgtgag ggtatggaaa tcgatctgtt aacggatatt
     3001 ccgacggttc ggacaagagg atagccatgt taatcgatgt tgttctggca ttcatgatta
     3061 tcgtgtcgtt tctgatagca atgattctgc atgaaatcgg ccattccctg gtcgccgcct
     3121 ggatgggcga tcctacaccg cgcaccgaag ggcgcatgag cttgagcctg gccccgcata
     3181 tcaacccgct cggtacgctg ctttgtgtca tcctggcctt cttcccggtg ggaagctccg
     3241 ttggcctggg atggggcaat ccggtcaagg ccgatccctg gaaattacgc ggcgggccaa
     3301 atgtgggtac gctcatcgtg gccctgggcg gcatcttcac cagcctgctg ctgggcggca
     3361 ttttcgcggg cgtgctgcgc attttgcctg ttgagctcta ctcgaatgcc ttcattgtgc
     3421 gcgtcccaca actgatcgca gtcttcgcca tcgtcaatat cagcctggcc atcttcaacc
     3481 tgatgccgct ctacccgctc gatggctatc agatactcta cagcctcttg ccaagccgcc
     3541 aggccgtcag ctttgcgaag agcgccccct atgggccatt catcatcctg ctgctgatct
     3601 tcctgctgcc gttcctgggg cagctcaccg ggctgagcga tttctttctg ttccgcatct
     3661 ccttctacat cctgcaaggg tcgctccaac ttgttggtct gattagtggg gcaggaggcg
     3721 caggcgcgtg tgcggtctat ctgggcctgg cgcctctggt gccattgcta tgtggctaga
     3781 caacatgcaa gcgttcacat atcgtctttg gcaggtgagc cagcaactgg gcttcgtcgc
     3841 gccgctctcc gaggatgagc ggcgcgaggt ggcgcgctgg ctgccggaat cggcctacgc
     3901 gctcttcacc agcatgtcgc ctgccgacca gcgtcacgcg ctgcgcgttt gccaggggtt
     3961 gcaggcgcgt ggctgtgctg aaccggacat gctggctgcc gcgctcttgc atgat
//
LOCUS       DASZAK010000397         2134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_649467, whole genome shotgun sequence.
ACCESSION   DASZAK010000397 DASZAK010000000
VERSION     DASZAK010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649467"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VGT44_06985"
     CDS             complement(<1..843)
                     /locus_tag="VGT44_06985"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEV2580583.1"
                     /translation="MLESTKTTRGTDQRETGQASRLTQNSSTGSNRSEGGSAPPGTSP
                     TRRVSWLRWLIFLIVINLLFYAPLLFSSLGAQQSTITLSYTNFLQQVDQGNVKEVTLT
                     GNNVSGDFKTPLRQPQSGTSSTTTSARFTSYVPETGDPSLLPTLEKNGVQVTAQPVQI
                     PWWQTALTILLNALPLLLLVYIGYMAMRSLRQSQQGGGIFGVGRSRAKLYNEERPSTT
                     FADVAGADEAKADLQEEIEFLREPQRYANIGAHIPKGVLLVGPPGTGKTLLARAVAGE
                     AGVPF"
     gene            complement(903..1166)
                     /locus_tag="VGT44_06990"
     CDS             complement(903..1166)
                     /locus_tag="VGT44_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6510 family protein"
                     /protein_id="HEV2580584.1"
                     /translation="MMENKLDGNAAAGILQAIFPFEMTLAQVTCTGCGATNAIGATAA
                     YMHGMGKVIRCPSCDNVLIRVALVRGHYLLDMRGVRVLQMKEG"
     gene            complement(1163..1945)
                     /locus_tag="VGT44_06995"
     CDS             complement(1163..1945)
                     /locus_tag="VGT44_06995"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580585.1"
                     /translation="MAQDMLAVSVRRAGDFWIFRIGQIISQIGSTLTSTALPLLVFKL
                     RGSALDLGLASAATWVPYALFGLFIGAWVDRVNRKRLMIGRDISRAVILAVLPVLALL
                     GHFSIWWIYLVTFINSTLTLGFQSSTTAAIPSMVPQDNLIVANGRIQASAPFATIIGP
                     LLAALLLTVASLQVVLWCDALTFVVSAISLTRGQPPGWTGYARRIDTQMLREVAWPVD
                     QRPLAYICGPTPFVETATASLVELGHEPVRIKTERFGPTGET"
BASE COUNT          441 a          615 c          639 g          439 t
ORIGIN      
        1 gaaagggacg cctgcctcgc ccgcgacggc acgagcgagc aaggttttgc cggtgcccgg
       61 ggggccgacc agcagcacgc ctttgggaat atgggctccg atattggcgt aacgctgcgg
      121 ctcgcgtagg aactcgattt cctcttgcag gtcggccttg gcttcgtcgg cgcctgccac
      181 gtcggcgaag gtggtggagg gacgctcttc gttgtagagt ttcgcacggc tgcgcccgac
      241 gccaaagatg cctcctcctt gctgcgactg tcgcaagctg cgcatagcca tgtagccgat
      301 gtagaccagc agcaggaggg gtaaagcatt gagcaggatc gtcagggccg tctgccacca
      361 gggaatttgt acaggctgag cggtgacctg gacgccgttt ttctccagtg tgggaagcaa
      421 gctgggatca cctgtctctg gcacatagct cgtgaagcga gcagaggtcg tcgtgctgct
      481 ggtgccgctt tgcggctgac gcagaggcgt tttgaaatcc ccggacacat tattgcccgt
      541 gagggtcacc tccttcacat tgccctgatc gacctgctgc aggaagttcg tgtaagacag
      601 ggtgatcgtc gactgctgcg cccccaatga actaaagagg agtggagcgt agaagagcag
      661 gttgatgacg atgaggaaga tgagccatcg cagccaggag acccgcctgg tgggggacgt
      721 tcctggcggt gcagacccgc cttcgcttcg gtttgatcca gtcgagctgt tttgggtcaa
      781 gcgagatgcc tgacccgttt cgcgttggtc agtgcctctc gtggtttttg tgctttccag
      841 catacggcct cctgtgttac gtgagaatga gaatggacca acttcgtcgt aggcgacctc
      901 cactaccctt ccttcatctg caggacacgc acgcctcgca tatccagcaa gtagtgtccc
      961 ctgaccagcg ccacgcggat gagtacattg tcgcaggagg ggcagcggat caccttcccc
     1021 atgccatgca tgtaggcggc agtcgctcca atcgcattgg tggcgccgca accggtgcag
     1081 gtgacctggg cgagcgtcat ttcgaacggg aagatggctt gcagaatccc tgcggccgca
     1141 ttgccatcca gcttgttttc catcatgttt ctcctgtcgg tccaaagcgt tcggtcttga
     1201 tgcgtactgg ctcatgtccc agttccacga ggctggcagt agccgtttcc acgaacgggg
     1261 tggggccgca aatatacgcc agcggacgtt gatcgacggg ccaggccacc tcgcgcagca
     1321 tctgggtatc gatgcggcgg gcatagcccg tccatcccgg cggctgccca cgggtcagcg
     1381 agatggctga gacgacgaag gtgagcgcat cgcaccacaa caccacctgc aacgatgcca
     1441 cagtcagcaa cagcgctgcc agcagaggtc cgatgatcgt ggcgaaagga gcgcttgcct
     1501 gaatgcgccc attcgccacg atcagattat cctggggaac catacttgga atagcggcag
     1561 tggttgagct ctgaaagcca agggtcagcg tggagttgat gaacgtgacg agatagatcc
     1621 accagatgga gaaatgtccg agaagcgcca gaactggcag caccgccagt atgaccgcgc
     1681 gggatatatc ccgaccgatc atcaagcgtt tccggttcac gcgatccacc caggcaccga
     1741 tgaaaagccc gaagagcgcg tatggcaccc acgtagctgc cgaggccaat cccaggtcca
     1801 gggccgagcc tctgagcttg aagaccagta ggggcagtgc cgtactggtg agcgtactgc
     1861 caatttgtga aatgatctga cctatccgga agatccagaa gtcgcctgct cttctcacag
     1921 aaacggccag catatcttgt gccatgacgt gttcgtctcc tggctctcag ctccttcgcg
     1981 agcatttcgt ctacccgatg agcaagagta tcctcatcag gggactggat gaattttccc
     2041 aacacctgac acaattatac tagcagtggc gagagaaatc accgtccatc agatggcatt
     2101 tttcttccct cacttctcac ccggcagaac ggcc
//
LOCUS       DASZAK010000398         2288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_578137, whole genome shotgun sequence.
ACCESSION   DASZAK010000398 DASZAK010000000
VERSION     DASZAK010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2288
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578137"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VGT44_07000"
     CDS             <1..1212
                     /locus_tag="VGT44_07000"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEV2580586.1"
                     /translation="PQDMTGAPQQYGVRLARLCRLLLGYRAPARSSKPTPTAAIVQAD
                     AVVHFLSRAYRFAIDGQLLALLPQDKEGALPTGSRDGAGVFDSGVEQLFAEAFASLGR
                     SNAADGWQLEREPEPILLPSHGIFIPDFALARDGHRLYLEILGFWTPSYRERKAQKLQ
                     QFKGRQDILLAMPGEARAAFASIASEFPIVEYEEQLSATEVLQVVRAHVDDFAERLAG
                     VDREQARHGIREAGWLPERAAFAALHCYRRSELAQVAEVIVDEAIKYLPGAGFYDQNW
                     LEQIGVSFVEWLGACEMDRIGGEGEESRKGRVLLQEALQMCRARWPVLAEGEDAALEA
                     LLGLVPGALIRRSSIFEAMIELEGISEAEVIQEQVSPSKKVVRERRVAYKKRPAGAAG
                     DSLASQQDLWG"
     gene            1229..>2288
                     /locus_tag="VGT44_07005"
     CDS             1229..>2288
                     /locus_tag="VGT44_07005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2580587.1"
                     /translation="MDIKELIGVTLGNCTLERILGRGGMGAVYLAQQARPIRMVAVKA
                     LLLPQNIDPQERRVFLERFRREADTAAKLEQNHILPIYEYSEAVVNGQQVAYLVMPYI
                     QGGTLRERIDQMNREGKRFDLRVIADYIGQVADALGYAHSLGVVHRDVKPANLLFHQD
                     GRLLLSDFGIARLNDMPSLTTAGNFLGTAEYASPEQANAGKLDARSDIYSLGVILYEL
                     LTGHVPFSASNPFAVLAKHMNEPAPSIRNARPDLSPSLEFVVKKALAKKPEDRYQSAP
                     EMAADLLAAISPVAPSARGMLRLGGDANNNDLTVADHLWQPPAYPVSPNDPVSPGRLA
                     SPAAVSFNAPALAPAQQAA"
BASE COUNT          448 a          597 c          776 g          467 t
ORIGIN      
        1 ccgcaggata tgaccggcgc gcctcagcag tatggtgtgc ggttggcgcg gctgtgccgt
       61 ttgctgctag ggtaccgtgc gcctgcgcgg tcgtcgaaac caacgccgac cgcggcgatt
      121 gtgcaggcgg acgcggtggt gcattttttg tcacgcgcct atcgttttgc gatagatggg
      181 caattgctgg cgttgttgcc gcaggataaa gagggagcgt tgccaacggg gtcgcgggat
      241 ggggcagggg tgtttgatag cggggttgag cagttgtttg cggaggcgtt tgcttcactt
      301 ggcaggagca acgctgctga tggctggcag ttggaacgcg agccggagcc gattttgctg
      361 ccgtcgcatg gcatctttat ccccgatttt gcattggcgc gcgacggaca ccggctctac
      421 ctggaaatcc tggggttctg gacgccatcg tatcgcgagc ggaaggcaca gaaattgcag
      481 cagttcaagg gacggcagga tattttgctg gcgatgccgg gcgaggcgag agcggccttt
      541 gccagtattg catcggaatt tcctatagtc gaatatgagg aacagctatc ggcgacggag
      601 gtgttgcagg tagtgcgcgc gcatgtcgat gattttgcgg aacggctcgc aggggtagat
      661 cgcgagcagg cgcgccatgg cattcgggaa gcagggtggc tgccggaacg cgcggcgttc
      721 gcggcgctgc attgctaccg gcgttcagaa cttgcccagg tggcggaagt catcgttgat
      781 gaggcgatca agtacctgcc cggcgcgggg ttctacgacc agaactggtt agaacaaatc
      841 ggcgtgtcgt tcgtagaatg gctaggggca tgtgagatgg ataggatcgg cggggagggt
      901 gaagagagca ggaagggtag agttctcctg caagaggcgc tgcagatgtg cagagcgcgt
      961 tggccggtgc tggcggaagg cgaagacgcg gctcttgaag ccctgctggg gctggtgcct
     1021 ggcgcgctca ttcgccgtag ttcgattttt gaagcgatga tcgagctgga aggtatttct
     1081 gaggctgaag ttatacagga gcaggtttct ccgtcaaaga aagtagtgcg cgagcggcgc
     1141 gtggcctata aaaagcgccc tgccggggcc gcaggcgatt ccctggccag ccagcaggat
     1201 ttgtggggat aaggaatcac aaaaacgtat ggatataaaa gagctgatcg gcgtgacgct
     1261 gggaaattgt acgctggaac gaatacttgg acgcggcggc atgggagcgg tctacctggc
     1321 gcaacaggcg cgcccgattc gtatggttgc cgtcaaagcg ctgttgctgc cgcaaaacat
     1381 cgatccgcaa gagcggcgcg tcttcctgga acgcttcagg cgcgaggctg atacggcggc
     1441 gaagctggag cagaatcata ttttaccaat ctacgagtac tctgaagcgg ttgtcaatgg
     1501 gcagcaagta gcgtacctgg tgatgcctta catccaggga ggtacgctgc gcgagcgtat
     1561 cgatcagatg aatcgcgagg gcaaaaggtt tgatttgcgc gttatcgcgg attatatcgg
     1621 acaggtggcg gacgcgcttg gttatgcgca tagcctgggc gtggtgcatc gcgatgtgaa
     1681 acccgccaac ctgctctttc accaggatgg gcggctgttg ctcagcgatt tcggcatcgc
     1741 gcgcttgaac gacatgcctt cgctcacgac cgccggaaat ttcctcggca ccgccgaata
     1801 tgcctcgccc gaacaggcca acgcgggcaa actggatgcg cgctccgata tctactcgct
     1861 tggcgtcatt ctctatgaac tgctgaccgg ccacgtgccg tttagcgcgt cgaacccgtt
     1921 cgcggtactc gcgaaacata tgaatgagcc ggcgccgtcc atccgcaatg cgcgccccga
     1981 cctctcgcca tcactggagt ttgtggtgaa aaaagccctg gcgaagaagc ccgaagatcg
     2041 ctaccagagc gcgccagaga tggcggctga tttgctggcg gcaatctcgc ctgtcgcgcc
     2101 gtcggctcgc ggaatgctgc ggctgggcgg agatgccaat aataacgacc tgaccgtcgc
     2161 cgatcacctc tggcagccgc cggcctaccc ggtcagtccg aatgaccccg tcagccctgg
     2221 gagattggcc agtcctgcgg ctgtgagttt caacgcgcct gccctggctc cggcgcagca
     2281 ggctgcac
//
LOCUS       DASZAK010000399         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_684896, whole genome shotgun sequence.
ACCESSION   DASZAK010000399 DASZAK010000000
VERSION     DASZAK010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684896"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VGT44_07010"
     CDS             complement(<1..332)
                     /locus_tag="VGT44_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580588.1"
                     /translation="MSLEGCAQSLSSSPTTTPVPTPRPQGSVYYTYRGHTGRITSVGW
                     SPNGKYIASGSLDQTVQVWAANPSDHFQPLIYRGHTAGVQTLAWSPDSNRIVSGSIDK
                     TVQVWDALT"
     gene            604..1200
                     /locus_tag="VGT44_07015"
     CDS             604..1200
                     /locus_tag="VGT44_07015"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEV2580589.1"
                     /translation="MKNTKPIPVGLCTAKRIMDSIYLDFINSEFRDFRGRWVRDDLQQ
                     PRWLEQFLIKWGLQVDRPLDASTLTTLIELRTLLRSMIEALVKGQLTGHDLAALNTVL
                     LKMPLNRQLLKDSEGYQLAIVPLKKDWDWVQAEVVASFAHLLAYHDPRRLKVCVNSNC
                     RWVFYDESKSRTRLYCSPDKCANLLKVRRFRARHKNNL"
     gene            complement(1212..2081)
                     /locus_tag="VGT44_07020"
     CDS             complement(1212..2081)
                     /locus_tag="VGT44_07020"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2580590.1"
                     /translation="MRYGVYVPNFGPYGNARVLADLAYEAEEAGWDGFFLWDQVSKTT
                     LTPTFDPMVDPWIALAAIALRTRTMRLGMLVTPLPRRRPWMLARETVSLDHLSAGRLI
                     CGVGSGGGSYDFEALGEATDAKTLAGLLDEGLEVLTGLWSGEPYRHEGTFYHIKEAQF
                     LPRPLQSPRIPIWVAGMWPAKAPFHRAARWDGVVPIGRDLPLTGMLTPAQMHEIVHSV
                     ASEPGYTLPFEIVHSGITGGTDTGHDREVVAAYQQVGVTWWVEKILPELWGGWTDWPL
                     EAMRGRIRRGPPK"
BASE COUNT          521 a          621 c          611 g          480 t
ORIGIN      
        1 gtaagcgcat cccacacctg taccgtttta tcgatggaac ctgagacaat gcgattgctg
       61 tctggtgacc aggccagcgt ttgcaccccg gcagtatgac cgcggtaaat caagggttgg
      121 aaatgatcgc tggggttggc ggcccacact tgcacggtct gatctaacga tcccgaagcg
      181 atgtacttgc cattgggtga ccaccctact gacgtaatac gaccggtatg accacgatag
      241 gtataataaa cacttccttg aggacgaggg gtaggaacgg gagtggtggt aggagatgaa
      301 gaaagagatt gagcgcaccc ctcgagactc atagcgatca tgaatcctgc cataccccgc
      361 agaacatcac ggcgagaggg gtatcccatg agtagaccca tttgtttacc tgccatcggc
      421 gcatcccttt cttgaggaga agcaagagct acaataaggg aacaagactg gcgatgtaac
      481 gttcaatgtt gtctaatata gttacatgta agagaatacc atgctaacca tgcgcctgtc
      541 aatatttttt agaacgttct accggtcttc ttcccacctc aaccgttgta tgaggtagga
      601 gacatgaaga atacaaagcc aattcctgta ggactctgta cggcaaagag gattatggat
      661 tcgatatacc ttgatttcat caatagcgag tttcgcgatt tccgaggccg ctgggtcaga
      721 gatgatctcc agcagccacg ctggttggaa cagtttctca tcaaatgggg gctgcaggtc
      781 gatcgcccac tcgatgcatc tacactcaca acccttattg aactgcgaac cctgctgcgc
      841 tccatgatag aagccctggt caagggacag ttgaccggac atgacctggc agcgctcaat
      901 actgttctac tcaagatgcc gttgaatcgt caactgctaa aagacagtga aggatatcaa
      961 ttggcaatag tgccgctgaa gaaagattgg gattgggtgc aggcagaggt agtggcttcg
     1021 ttcgctcatt tgctggctta tcacgatccc agacgtctca aagtgtgtgt aaatagcaac
     1081 tgccgttggg tcttttatga tgagagtaag agtcgtacca ggctttactg ctcgcctgat
     1141 aaatgcgcta acctgttgaa ggttcgccgt ttccgtgctc gccataaaaa caatctctaa
     1201 gaatgcctca cttatttcgg cggcccccgt cgaatccgcc cgcgcatcgc ctcaaggggc
     1261 cagtcggtcc agcctcccca aagttcgggc agtatctttt ccacccacca tgtgacgccc
     1321 acctgctggt acgcggcaac gacctcgcga tcgtgtcccg tgtccgtgcc tccggtgata
     1381 cccgaatgta cgatctcgaa cgggagagtg tatccaggct cggatgcgac agagtggacg
     1441 atttcatgca tctgagccgg ggtgagcatc ccggtgaggg gcaggtcgcg cccgataggg
     1501 acgacgccat cccagcgggc cgcgcggtga aacggcgctt tcgctggcca catacccgcc
     1561 acccagatgg ggatgcgcgg agactggagc ggacgcggaa ggaactgtgc ttccttgata
     1621 tgataaaaag taccctcgtg tcgatatggc tcgccgctcc aaagccccgt gagcacctca
     1681 agaccctcgt ccagcagtcc cgccagcgtt ttggcatccg tcgcttcgcc caacgcctcg
     1741 aagtcgtaag agccacctcc cgagcctacc ccgcagatca gccgccctgc tgacaggtga
     1801 tccagcgaca cagtttctcg cgcaagcatc caggggcggc gacgtggcag gggcgtgacg
     1861 agcattccca ggcgcattgt gcgcgtgcgc agcgcaatcg cggccagagc aatccaaggg
     1921 tcaaccatgg gatcgaaggt cggtgtcagg gtcgtcttcg acacctggtc ccagagaaaa
     1981 aagccgtccc agcccgcctc ttccgcctca tacgccaggt cggcaagcac ccgggcattt
     2041 ccatagggtc caaaattggg aacgtagaca ccataccgca taggaaactc ctcaatctca
     2101 gcagtgacta ccgtctctgc gccctcctca tccaggagcg cagccttgtc attgtatcat
     2161 atgctcaata cccgtagggg cgagggtggt gtggttggga agagtgggcc tttatggtcg
     2221 cccgacgtgg gtc
//
LOCUS       DASZAK010000400         1717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_222960, whole genome shotgun sequence.
ACCESSION   DASZAK010000400 DASZAK010000000
VERSION     DASZAK010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222960"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1344)
                     /locus_tag="VGT44_07025"
     CDS             complement(22..1344)
                     /locus_tag="VGT44_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine synthase"
                     /protein_id="HEV2580591.1"
                     /translation="MALNSNRRYGFETLSLHAGQETADSATNARAVPIYQTSSYVFDS
                     PEHASDLFALKQFGNIYTRIMNPTQDAFERRIAALEGGVGALATASGQAAETLAILNI
                     AQTGDEIVTSASLYGGTYNLFHYTLPKLGINVRFVDSRNPENFRPAINDRTRAIFAET
                     VGNPRLDTLDIRAVADIAHEYGLPLILDNTLPTPYLVQPFKHGADIVVHSATKFIGGH
                     GTAIGGVIVDSGTFDWTSGRFPEFVEPDQSYHGLRYVEALGPLAYIIKARVQLLRDLG
                     PATTPFNSWLFLQGLETLPLRMERHSQNAQRIAEFLEGHSSVNWVSYPGLKSHPQYEL
                     AAKYHTHGYGAILGFGIKGGLEAGKQLIRHVELFSHLANVGDAKSLIIHPASTTHSQL
                     TPDEQYETGVTPDFVRLSVGLETVDDLIEDLDQALKAATLKASESLSV"
     gene            complement(1353..>1717)
                     /gene="metX"
                     /locus_tag="VGT44_07030"
     CDS             complement(1353..>1717)
                     /gene="metX"
                     /locus_tag="VGT44_07030"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911744.1"
                     /note="Catalyzes the conversion of acetyl-CoA and
                     L-homoserine to CoA and O-acetyl-L-homoserine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEV2580592.1"
                     /translation="FTDLGERFDVEGYLYYQGEALVRRFDANSYLYISRAMDLYDVSE
                     GYPSLEAALSRLRSKALFIGIRSDFLFPAARVRWLADQVRAAGGEAIYAELDSPHGHD
                     AFLKEWEQMTGALNLIKE"
BASE COUNT          378 a          452 c          515 g          372 t
ORIGIN      
        1 acatccacat cgtccccgcc attacacgga aagggactcg ctagctttga gggttgcggc
       61 ttttaaggct tgatcgaggt cttcgatcag gtcatcgacc gtctccaggc cgactgatag
      121 gcgaacgaaa tcaggtgtga cgcctgtttc gtactgctca tcgggtgtca attgtgagtg
      181 ggtcgtgctc gccggatgga tgatgagcga cttggcgtct ccgacgttgg ccaggtgaga
      241 gaatagctca acgtgacgaa tgagctgttt gcccgcctcc aggccgccct tgatgccgaa
      301 gccgaggata gccccgtaac catgcgtatg gtactttgct gctagctcgt actgcgggtg
      361 gctcttcagg ccaggatagc tgacccaatt gacagacgag tgcccctcca ggaactcggc
      421 gatgcgctgg gcattctggc tgtggcgctc catacgcaat ggtagggttt ccaatccttg
      481 caagaagagc caggagttga acggcgtggt ggcagggccg aggtcgcgca gcagttgtac
      541 acgcgccttg ataatgtaag ccagcgggcc aagcgcttcg acgtaacgaa gtccatgata
      601 cgattgatca ggctcgacaa attcggggaa gcgcccgctc gtccagtcga acgtcccgct
      661 atcgacgatt acaccgccga tagctgtgcc gtgcccacca ataaacttgg ttgccgagtg
      721 gacaacgata tcagcgccgt gcttgaacgg ctgcaccagg tagggcgtgg gtaaggtatt
      781 atcgagaatg agcggcaggc cgtattcgtg cgcgatgtcg gctacagcgc gtatatccag
      841 ggtgtccagg cgcgggttgc cgactgtttc ggcgaagatg gccctggtgc gatcattgat
      901 ggcagggcgg aagttctccg ggttgcggct gtccacgaag cgaacattga tacccagctt
      961 gggaagcgta tagtggaaca ggttataagt gccgccgtac aggctggccg aggtgacgat
     1021 ttcatcaccc gtctgagcga tgttgagaat agcaagggtt tccgcagcct ggccagaagc
     1081 ggttgccagt gcgccaacac caccttcgag ggcggctata cggcgttcga aggcatcctg
     1141 ggtgggattc ataatgcgcg tgtagatgtt gccaaactgc ttgagcgcga ataggtcgga
     1201 ggcatgttcg ggactatcga agacatacga gcttgtctga tagatgggaa ccgcacgtgc
     1261 attagttgcg ctgtctgctg tttcctgccc agcatgcaaa gataacgttt caaatccgta
     1321 ccggcgatta ctgttaagtg ccataggtgt tgttactcct ttatcagatt cagcgcaccg
     1381 gtcatttgct cccactcttt caggaaagca tcatggccgt gcggggaatc aagttccgca
     1441 tagattgcct caccacctgc agcgcgcacc tggtcggcta accagcgcac acgcgcggca
     1501 ggaaaaagaa aatccgaacg aatgccgatg aagagcgctt tactacgcaa gcgactcaac
     1561 gcggcttcaa gtgagggata gccctcactg acatcataca aatccattgc ccggctgatg
     1621 tagaggtagc tattggcgtc gaagcgcctg accagcgctt caccctggta atagaggtag
     1681 ccttctacgt cgaagcgctc gccaagatcg gtgaaac
//
LOCUS       DASZAK010000401         2706 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_684922, whole genome shotgun sequence.
ACCESSION   DASZAK010000401 DASZAK010000000
VERSION     DASZAK010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684922"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VGT44_07035"
     CDS             <1..1117
                     /locus_tag="VGT44_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580593.1"
                     /translation="IEQCGNAPRRYRNGLSLAIPDGHKIDVIRNGVRLILTLELLQSQ
                     SKQRQLTPQQEAELTERKRNAENELRGGISLLYAYVYTPQNSEQVGLNYTFDKLQVQS
                     YSQAPQVHSRVKEALHNRVVWDSVQPSKLAKLTQLNELQPLEKQYYAVVALVACFFSY
                     YNFTHIWDEKVIRQAIIVGVKNRTFAYVANARKDNHENLVFGSPAATSVYFGKDIPAH
                     EIDMGEGAFLLSAAYAQQLLTPPAPTKIEPPDVPTIHDGPQIYSGSPTQAGVATGHVA
                     PPPLIPSLPQPTTQPVAPGQGGQHFRLRLQAKSEDVFEVMKALGNLGGLIESMQIAIV
                     ATAKTGQPFIPNKIHNLVVEPMTEESHVQVLEEQVEE"
     gene            1201..1611
                     /locus_tag="VGT44_07040"
     CDS             1201..1611
                     /locus_tag="VGT44_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV2580594.1"
                     /translation="MMDQRNYNHQLIEEFRANRHKAGGSFEGRPLLLLTTTGARSGKR
                     RTTPMMYVRDGERLLVIASNAGAPAHPDWYFNLVAHPQVAVEVGTETFDATAVVTEGE
                     ERQRLWTRIVELYPFFAQHQAKITRQIPVIALSR"
     gene            complement(1618..2652)
                     /locus_tag="VGT44_07045"
     CDS             complement(1618..2652)
                     /locus_tag="VGT44_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580595.1"
                     /translation="MAQLVLGIGMSHSTMVTLDDSLWEEWAALDAGFAILYDTEGQPV
                     TYAQLAERAGDRYSRLATPRHWKEQYAAVRRAVARLAADVAGARLDVLVVVGDDQLEL
                     FSFSNMPALAIFYGDRLTSGLWTSRFAAYQRQGQPPATPLSQELQRALVEGYAMESHH
                     QFSSAPAFARALLEGLIERGFDVAGLAEFPTNYESAGIGHAYGAIATQLLGEQLIPMV
                     PVLVNTYFPPNRPTPSRCYDLGLALSQAIESISGDLRVGIVASGGLSHFVTDEQLDRR
                     LFNALRAGDEAELRAIPQKLLNAGSSEIRNWIAAAAACKHLKLAWDEYIPVYRTLAGT
                     GCGLGFARWS"
BASE COUNT          645 a          810 c          743 g          508 t
ORIGIN      
        1 tatcgagcaa tgcggaaacg cgccgcgccg ctatcgcaac ggcctgtcct tagccattcc
       61 tgatggtcac aagatcgatg tgatccgcaa cggggtgcgc ctgatcctga cactggaact
      121 cttgcagagc cagagcaaac aacgccaact gacgcctcaa caggaagctg agcttaccga
      181 acgcaagcgc aacgcggaaa atgaactcag aggtggtatc tcgctgctct acgcatacgt
      241 ctacacgccg cagaatagcg agcaggtggg gctgaactac acatttgaca aattgcaggt
      301 acagagctat agccaggctc ctcaggtgca ttcgcgcgtc aaagaagccc tgcacaatcg
      361 cgtggtctgg gacagcgtgc agcccagtaa actggcaaag ctgacacaat taaacgaatt
      421 gcagccgctc gaaaagcaat actacgctgt tgttgctctg gtggcttgct tctttagcta
      481 ctacaatttc acacatatct gggacgagaa ggtgatacgc caggctatta tagttggtgt
      541 taaaaaccgc accttcgcct atgtcgctaa cgcgcgcaaa gacaaccatg aaaacctggt
      601 attcggcagt ccagcggcga cttcggttta ctttggcaaa gacatccccg cgcatgaaat
      661 cgatatgggt gaaggcgcat ttctgctttc ggcagcctat gcgcaacaat tgctcacgcc
      721 gccagcgcca acaaagattg agccgccaga cgttccgact attcatgatg gccctcaaat
      781 atactctggc tcacctaccc aggcaggagt agctaccggg catgttgccc caccaccact
      841 tatcccatcc ttgccacaac ccacaacaca accagtagcc cccggacagg gcgggcaaca
      901 tttccgcctg cgcctccagg caaagtcgga ggatgtcttt gaggtaatga aagctctcgg
      961 aaacctgggt ggcctcatcg agtcgatgca aattgccatt gtcgctaccg ccaaaaccgg
     1021 tcagcccttc attccaaaca agatacacaa cctggttgta gagcctatga ccgaggaaag
     1081 ccacgtacag gtattagaag aacaggtcga ggaataaaat ggatgacaaa tcgccaagag
     1141 gaaggtatgt atgagcagtg acgcgacact tgctcgctgg catcgacaaa agaagggatc
     1201 atgatggatc aacggaacta taaccaccaa ctgattgagg aatttcgcgc gaaccgccac
     1261 aaggctggag gatcgttcga gggccggccc ttgctcctgc tcacaaccac cggcgccagg
     1321 agtggaaagc gccgcacgac gccaatgatg tatgtccgcg atggcgagcg cctgctcgtg
     1381 atcgcctcta acgccggcgc accggctcat cctgattggt attttaacct tgtagctcac
     1441 ccacaggtcg ctgtagaggt cgggaccgag accttcgatg ccactgccgt agtgacggaa
     1501 ggggaggagc gacaacggct gtggacccgg atcgtagaat tgtatccctt tttcgctcag
     1561 caccaggcga agattactcg gcagatccca gtcatcgcgc tttcccgata aaaagctcta
     1621 cgaccagcgc gcaaagccca gcccgcaccc ggtaccggca agcgtgcgat agacggggat
     1681 atactcgtcc caggccagtt tcaggtgctt gcaggcggcg gcggcggcga tccagttgcg
     1741 aatctccgaa cttccggcat tgagtaattt ctgagggatg gcgcgtagtt ctgcctcgtc
     1801 gcctgcgcga agggcattga acaggcgacg atcaagttgc tcgtcggtga cgaagtggct
     1861 caacccaccc gaggcaacga tacccacgcg cagatcgcct gagatagact cgatggcctg
     1921 cgacagcgcc agtccgagat cgtagcagcg cgaaggcgtg ggccggttcg gtggaaaata
     1981 ggtattgacc agcacaggaa ccatagggat caactgctct cccaagagct gagtcgcgat
     2041 ggccccgtag gcgtggccga tgcctgccga ctcatagttc gtggggaact cggccagccc
     2101 tgccacgtcg aagcctcgct cgatcaagcc ttcgagcagg gcacgagcga aggctggcgc
     2161 gctgctgaac tggtgatggg actccatagc atagccctcc accagggcgc gctgaagctc
     2221 ctggctcagc ggtgtcgcag gcggctgacc ctgcctctgg tacgcagcaa agcgcgatgt
     2281 ccacagccca ctcgtgagcc tgtctccgta gaaaatagcg agcgcgggca tattcgagaa
     2341 cgaaaacagc tccagctggt cgtcccccac gacgaccagc acatccagcc gcgctccggc
     2401 tacgtcggca gccaggcgag cgaccgcccg ccgcacggct gcgtactgct ccttccagtg
     2461 ccggggtgta gcaagccgcg agtagcggtc tcccgctcgc tcggcgagct gcgcatacgt
     2521 caccggctgt ccctcggtgt catacaggat cgcgaagccc gcatccaggg ctgcccactc
     2581 ttcccaaagc gaatcatcca acgtcaccat cgtgctgtgg gacataccta ttccaagaac
     2641 aagttgtgcc ataatgaatc actcctttgt tctgatcggc ccctagaagc aagtgctatt
     2701 gcgcct
//
LOCUS       DASZAK010000402         4352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_223056, whole genome shotgun sequence.
ACCESSION   DASZAK010000402 DASZAK010000000
VERSION     DASZAK010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4352
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223056"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2661)
                     /locus_tag="VGT44_07050"
     CDS             complement(<1..2661)
                     /locus_tag="VGT44_07050"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HEV2580596.1"
                     /translation="MDGKITYHQQVSYCGKPRCRRCREGTGHGPYWYAYQTINGRTVR
                     TYVGKNPPPEALQAAGAFEASEDTREANAAAAPDAVVRLSVLGQFTLERRDTQGGRTG
                     SARWQAVTDAALQHQRVRSLLTCLISSPGRKLGREQAIELLWPDLDFETASHRLDKAV
                     HSLRQVFEPGRTRPATSDLLLTEHSTLQLAEQAQLWIDADAFDALLAQARASNDPGKT
                     EQLLEEALLLYGGEYVPDEREQDAAQARRESLQRSWIGLMLELADLRIAREAFPAAID
                     TLDHLLAIDPTSEAAVQRLVLLLSQTGRRAEALRIYQRFVSLLKQEYRMAPLPETRAL
                     YEAARRGSDPATASTLGDTPARGSGNARASNLNEAPSEAPGSPLAGILTPTQEAARVV
                     EVESHTHMQVGRSNQSRLVGREQELAALQQMLRLSEQTRRVKLSGQRKSIATPDMRRS
                     PQCLLLMGDVGIGKTRLAEELGREARRRGWTIAWTRAYAQEINIPYRLWTESLRKAMS
                     QGLWQRQEIARHPLLYSALRALLPELDELLPQMEPTTITTSPEQEQLRLWEAARALLD
                     TISESAPLLIVLDDLQWADSSSCELLAYLVRQLRGQPVLIAGTCRDIELPPTHPLRTV
                     LNDLQREQAIETITVQPLSDEQIRALVSGLPEPAVQHIQTRAAGNPFFAEELARNVVL
                     PDPPATSSDITPVTMPDTISAVLDLRLGRISSACQRMLVRAAVLGGSFEFNLLRAMES
                     GPTALDEDALLDLLEEALQTGMITEEGSGTRITYHFWHPLLVSHLYDGLSAGRRASLH
                     RRAAEVLRAAHAGRAEEFAAAITYHLLNGGADSRDIARYAELAGDRAYALSAYPEAAR
                     HYRIAVEHIGTLPAGASSEECLRLANLLERL"
     gene            3373..>4352
                     /locus_tag="VGT44_07055"
     CDS             3373..>4352
                     /locus_tag="VGT44_07055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2580597.1"
                     /translation="MSATQVIADSSKGRQDQIEKLRALAAMTGERSLTSDSNRLYRDE
                     IGQVDLLSNEDVICLAQRMERGRAAAEYPRHPGHAKLIEEGELARRKLIEANLRLVIS
                     VARKYRGLDIDMMDLIQEGNLGLIHAVEKFDYTKGYRFSTYAIWWIRQAITRALTEQA
                     RMIRVPLHKMEKMKRLERVQHRLRDGQDVEPSLDDLAEEMELSQQQIIDLMTLIRSQE
                     PLSLDIRRKVGDDDLPLSDLLEDDQAYSPEQIVMTQTLEQQVRDLLESLTLRERQVIR
                     YRYGLDGTREHTLHEVGRKLGLSHEAVRQVESRALRKLDPLSRNRKLEEF"
BASE COUNT          821 a         1253 c         1421 g          857 t
ORIGIN      
        1 gagtctttcc agcagatttg ccaggcgcaa acattcttct gagcttgccc cggctggcag
       61 tgtcccgata tgttctacgg cgatgcggta atggcgggcg gcctccgggt aagcagacag
      121 ggcgtaggcg cggtctccgg cgagttcggc gtagcgggcg atgtcgcggg agtcggcgcc
      181 gccattgagc aggtggtagg tgatggcggc ggcgaattcc tctgcgcggc cagcgtgggc
      241 ggcgcgcagg acttcggcgg cgcggcggtg caggctggcg cgcctgcccg cggagaggcc
      301 gtcgtagagg tggctgacca gcagggggtg ccagaaatgg taggtgatgc gcgtaccgct
      361 gccctcttcg gtaatcatgc cggtctgcag ggcttcttcc agcaggtcga gcagcgcatc
      421 ttcgtcgagt gctgtgggac cgctctccat ggcgcgcagc aggttgaact cgaaggagcc
      481 gcccagcacc gccgcgcgta cgagcatacg ctggcaggcg ctgctgatgc ggcccaggcg
      541 caggtcgagg acggcggaga tggtgtcggg catggtgaca ggcgtgatat cggacgaggt
      601 ggcaggcggg tcaggcagca cgacgttgcg ggcgagttct tcggcgaaga aggggttgcc
      661 cgccgcgcgc gtctggatat gctgcacggc gggttcgggc agaccggaga cgagcgcgcg
      721 aatctgctcg tcgctgagcg gctgcactgt gattgtttcg atggcctgct cgcgctgcaa
      781 atcattcagc acggtgcgca atgggtgtgt aggcggcagt tcgatgtcgc ggcaggttcc
      841 ggcgatcagc acaggttgac cgcgcagctg gcgcaccagg taggccaata gctcgcagct
      901 gctgctgtcg gcccactgca ggtcgtccag cacgatcaac agtggagcgc tttcgctgat
      961 ggtatcgagc agggcgcgag cggcctccca caggcgcagc tgctcctgtt ccggcgatgt
     1021 tgtgatggtg gttggctcca tctgcggcag cagttcgtcc agttcgggca gaagggcgcg
     1081 cagggcgctg tagagtaatg gatggcgggc gatctcctgg cgctgccaga gtccctggct
     1141 catggccttg cgcaggcttt cggtccagag acggtagggg atgttgattt cctgggcata
     1201 agcgcgcgtc caggcgatgg tccagccgcg ccgccgggct tcgcgcccca gttcttcggc
     1261 gagacgtgtt ttgccgatgc cgacatcgcc catcagcagc aggcattggg gcgagcgtcg
     1321 catgtcgggc gtcgcaatgc tcttcctctg gccggacagt ttgacgcgcc tggtctgttc
     1381 cgacaggcgc agcatttgct gcaaagcggc cagctcttgc tcgcgcccga ctaaccggct
     1441 ctggttgctg cgtccgacct gcatatgcgt gtgcgactcc acctcaacca cgcgcgcggc
     1501 ctcctgcgtg ggcgtgagta tgccggcgag cggactgccc ggcgcttcgc tgggtgcctc
     1561 gttcaggttg ctggcgcgag cattgccgga gccgcgcgcg ggggtgtcac cgagcgtact
     1621 cgcggtcgcg ggatcgctgc cgcgccgtgc ggcctcgtag agcgcgcgag tttcgggcag
     1681 gggcgccatg cgatattcct gtttcaagag gctaacgaaa cgctggtaga tgcgcagtgc
     1741 ctcggcgcgc cggccggttt gcgataacag cagcaccagg cgctgtacag cggcttcact
     1801 ggtcgggtcg atggccagca ggtggtcgag ggtatcaatt gcggcgggga aagcctcgcg
     1861 ggcgatgcgc aggtcggcca attcaagcat caggccgatc caactgcgct gcagcgactc
     1921 gcggcgcgcc tgggccgcgt cctgttcgcg ttcgtccggc acatattcgc cgccataaag
     1981 cagcagagct tcttcgagca gctgttcggt tttgccaggg tcattgctgg cgcgcgcctg
     2041 tgccagaagc gcgtcgaagg cgtcggcgtc gatccataac tgcgcctgct ccgcgagctg
     2101 cagtgtggag tgttcggtga gcagcaggtc tgaggtagca gggcgggtgc gtcccggttc
     2161 gaagacctgg cgcaggctat gcaccgcctt gtcgaggcgg tggctggcgg tctcgaagtc
     2221 aaggtcgggc cagagcagct cgatggcctg ctcgcgccca agcttgcggc cagggctgct
     2281 aatcaaacag gtgagcagcg aacgcacacg ctggtgttgc agcgccgcgt cagtcaccgc
     2341 ctgccagcgc gcgctgcctg tccgaccgcc ctgggtgtcg cgccgttcga gtgtgaactg
     2401 gcctagcacg ctcaggcgca cgacggcgtc gggcgctgcc gccgcgttcg cttcccgcgt
     2461 atcctcgctc gcctcgaatg ctcctgccgc ctgcaatgcc tctggcggcg gatttttacc
     2521 cacgtaggta cgcacggtac gcccattgat cgtctgatag gcataccagt aggggccatg
     2581 tccggttccc tcgcggcagc ggcggcagcg cggtttaccg cagtagctca cctgctgatg
     2641 gtacgtaatt ttaccgtcca tttcgaaacg gtctcctcaa tttcctgagc aaagccagcg
     2701 tatgcacatt attatatcaa actacatcaa tttgagtcac atttcagcct gatagccatg
     2761 atgccccttt tgaagcaatc tttcagatag gtgagtgaac atccccgttc ccccatttac
     2821 aaataaacct gccctcaaca caaaattgcc cgatttgccg ataaaaaggc aacataagac
     2881 cggtataaga catattttct ggatgcctcg cccatatcta cggataccga ccgccgaatg
     2941 tccacaaggg gtatttctat tgtcatatcc tggtcaagct tatcccaggt gccatctcta
     3001 caaaacaaac actaacatcg gtgtagtaag ccacagggcg accgcaaggg tctctacccc
     3061 actccgcgcc actcccgccc ttacaaagag gacggattgg cttattgagg gtcttcgtag
     3121 tctttgtaag ggcgggagtg gcgcggagtg gggtggagac ccttgcggtc gccctcgttc
     3181 tcgccgtatt gctggccgat atatcgtacc tgttgccaat ttgtcaacca ggaaaccgca
     3241 tggaagtacg ctgggacata ccggaaagac ccgcgccgta cacttgtcct cagaacacgg
     3301 gcggatgctg ggagaattgt cccgtggact atgccatttg cgaagaatga tagcagaaag
     3361 atggaggaac acatgagcgc gactcaggtc attgcggact cgtcaaaggg acgccaggat
     3421 cagatcgaaa agctcagagc gctggcggct atgaccggag aacggtcgtt gaccagtgat
     3481 tccaaccggc tctatcgcga tgagatcgga caggtcgatt tgttgagcaa cgaggatgtt
     3541 atttgcctgg cgcagcgtat ggaacgcggg cgcgctgccg ccgagtatcc ccggcaccct
     3601 ggccacgcga aattgataga ggaaggagaa ctggccaggc gcaagctgat cgaggccaat
     3661 ctgcggctgg ttatttcagt ggcacgcaag tatcgcgggc tggatataga catgatggac
     3721 ctcatccagg agggcaatct gggactgata catgcagtag agaagtttga ttatacaaaa
     3781 ggctaccgtt tcagcactta tgccatctgg tggatacgcc aggccatcac gcgcgcgctg
     3841 accgaacagg cgcgcatgat tcgcgtaccg ttgcataaga tggagaagat gaaacggctt
     3901 gagcgcgtgc agcatcgcct gcgcgacggt caggatgtcg agccgtcgct cgatgacctg
     3961 gcggaagaga tggaactgtc ccagcagcag atcatcgatt tgatgacgct gatacgctct
     4021 caagagccgc tcagcctgga cattcgccgc aaagtcggcg acgatgactt gcccctcagc
     4081 gacttgctgg aagacgacca ggcgtattcg ccagaacaga tcgtcatgac gcagacgctg
     4141 gaacagcaag tccgcgatct actcgaaagc ctcacgctgc gcgaacgcca ggtcatccgc
     4201 taccgctatg gcctggacgg tacgcgcgag catacgctgc acgaggtagg ccgcaaactc
     4261 ggtctgagtc acgaagctgt ccggcaagtc gagagccggg cgctgcgtaa acttgatcca
     4321 ttgagccgta atcgaaagtt ggaagagttt tt
//
LOCUS       DASZAK010000403         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_436661, whole genome shotgun sequence.
ACCESSION   DASZAK010000403 DASZAK010000000
VERSION     DASZAK010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436661"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VGT44_07060"
     CDS             <1..714
                     /locus_tag="VGT44_07060"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2580598.1"
                     /translation="VLLAWDAETATGLPPLGNDYVAAIVQRYPQQFIGFASVDPHKGK
                     AAVKEFERAIKDLGLAGAKFHPGVQAFYPNDPTFYPLMEKIQELGVPALFHTGTNGLG
                     AGTPGGMGIKLDYTRPIYLDALAADFPGLTIIGAHPSWPWHEEMIAIVQHKANVFNDL
                     SGWSPKYTPDSLLREAATRLQDRILFGSDYPFITPQRWLKDFDALDYFKPEVREKILW
                     RNAQKLLAHTSVGHMHFSS"
     gene            complement(777..>2099)
                     /locus_tag="VGT44_07065"
     CDS             complement(777..>2099)
                     /locus_tag="VGT44_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2580599.1"
                     /translation="INRAVTAARKAFESGPWPKMTPSQRGRLLWKVAELIEQHADELA
                     QLETLDNGKPIKYSKGGDVPLTADHFRYFAGWATKIEGDTIPVSIPNMFTYSLREPLG
                     VVGQIIPWNFPLQMASWKLAPALACGNTVILKPAEQTPLTALRLGELICEAGFPDGVV
                     NIVPGFGETAGAALAAHPDVDKIAFTGSTEVGKKILQASAGNLKKVTLELGGKSPNII
                     FPDADMKYAVRGALNAIFFNQGQVCTAGSRLFVHASVYDQVVSGLTDAASKMKLGQGI
                     DPATELGPVVSQEQLERVTGYIEIGRREGATPTTGGERATGDLAQGYFVQPTVFDQVR
                     DDMTIAREEIFGPVVVAMPFEDIEEVAARANRSIYGLAAGVWTSDIKKAHKMVSLLKA
                     GVIWVNTYNQFDAAAPFGGYKQSGYGREMGHAVLDAYTQVKTAWINLR"
BASE COUNT          415 a          645 c          592 g          447 t
ORIGIN      
        1 gtcctgctgg cctgggacgc cgaaacggca acggggttgc ccccgctggg gaacgattat
       61 gtcgctgcca tcgtgcagcg ctatccgcag caatttatcg gctttgccag cgtagatccg
      121 cacaaaggga aggcagccgt caaggaattc gaacgtgcta tcaaagacct ggggctagca
      181 ggagcaaaat tccatccagg cgtccaggcg ttctatccca acgatcccac gttttatcca
      241 ctgatggaga agattcaaga actgggcgtg cctgcactct tccataccgg tacgaacggg
      301 ctgggagcag ggacacccgg gggcatgggc atcaaactcg attacacgcg ccccatctac
      361 ctggatgccc tggcagccga ttttcctggc ctgaccatca tcggcgcgca cccctcctgg
      421 ccctggcacg aagagatgat cgcaatcgtg cagcataagg ccaatgtgtt caatgacctc
      481 tctggatggt caccgaagta cacgcccgac tcgctcctac gcgaggccgc cacccgcctg
      541 caagatcgca tcctctttgg ctccgactac cctttcatca caccgcagcg ctggctgaaa
      601 gacttcgatg ccctggacta cttcaagcca gaggtgcgtg agaaaatact gtggcgcaat
      661 gcccagaaat tgctggctca taccagtgtt gggcatatgc acttctcttc ctaacgcgct
      721 ggtggatgag gcatatgacg gtctcgctgc ctttacccca aaatcaaact catacctcag
      781 cgcaggttaa tccaggctgt cttgacctgc gtgtaggcat ccagcacggc atggcccatt
      841 tctcgtccgt agccgctttg cttgtagcca ccgaaagggg cagccgcgtc gaactggtta
      901 taggtgttga cccagatgac accggctttg agcagggaga ccattttgtg cgccttcttg
      961 atgtcgctcg tccagacacc ggctgccagc ccgtagatcg accggttggc gcgcgcggct
     1021 acttcctcga tatcttcaaa aggcatggca accaccaccg ggccgaagat ctcttcgcgg
     1081 gcgatggtca tgtcgtcacg cacctggtcg aaaacggtgg gctgcacgaa gtatccttgc
     1141 gccaggtcac cggtggcacg ttcaccccct gtcgtcggcg tcgccccctc gcgcctcccg
     1201 atctcgatat agccggtcac acgctccagt tgttcctgcg agaccacggg acccagttct
     1261 gtggctggat caatgccttg ccccagcttc atcttgctgg cagcatccgt caacccgctc
     1321 actacctggt catacacgga cgcgtgaacg aagaggcggc tgccagcagt acagacctga
     1381 ccctgattaa agaagatcgc gttaagtgct ccgcgtaccg cgtatttcat atcggcatcg
     1441 ggaaagatga tgttgggtga cttgccaccc agttccagag tcaccttctt caaattgccg
     1501 gcgctggcct gcaaaatctt cttgccgact tctgtcgagc cggtgaaggc gattttgtcc
     1561 acatcagggt gagctgccag cgccgcgccg gccgtctcgc cgaagcctgg cacgatattg
     1621 accacaccat cggggaagcc tgcctcgcag atcagttcac ccaggcgcag ggccgtcagt
     1681 ggcgtttgct ccgccggttt gaggatgacc gtattgccgc aggccagagc cggggctagt
     1741 ttccaactcg ccatctgcaa ggggaagttc cagggaatga tctgcccgac aacgccgagt
     1801 ggctcgcgca gcgagtaagt gaacatgttg gggatggaga cgggtatggt gtcaccctcg
     1861 attttggttg cccagcccgc gaagtagcgg aaatgatcgg cggtcagcgg cacatcgccg
     1921 cccttcgagt atttgatggg cttgccgttg tccagcgtct caagctgtgc cagttcatcg
     1981 gcgtgctgct cgatcagttc ggctactttc cagagcaatc gcccccgctg tgagggcgtc
     2041 attttcggcc aggggccgct ctcaaaggct ttgcgagccg cggttactgc gcggttgat
//
LOCUS       DASZAK010000404         7016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_293201, whole genome shotgun sequence.
ACCESSION   DASZAK010000404 DASZAK010000000
VERSION     DASZAK010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293201"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..521
                     /locus_tag="VGT44_07070"
     CDS             <1..521
                     /locus_tag="VGT44_07070"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEV2580600.1"
                     /translation="MEGSNVNKQDIEKYLRMLGQELLHRQVTGEILLVGGAVMLLKVQ
                     NRETTKDIDAYFVTGQAEIIREAAQAIADREGLAQDWINDAAKGFFYTQPSTEQWAEY
                     PGLRVYMPSLDYIFAMKVVAGRPQDIDDIKALAQELNLSNARDALTIVKEYIPEQLLV
                     PRVEYMIDEIFE"
     gene            561..962
                     /locus_tag="VGT44_07075"
     CDS             561..962
                     /locus_tag="VGT44_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580601.1"
                     /translation="MAQTQPKWQPQTAAETFERICNNTTPWVAIGDFLDDWRRTPVEH
                     RHELATEPIASAPTPELQRWAAFCAAMVEWLCWQDHLPFPSWTSQATYRLPQPWFLYK
                     GWKLRAWQLATTPTPFKMRNIFGGDRMLDRV"
     gene            complement(975..1913)
                     /locus_tag="VGT44_07080"
     CDS             complement(975..1913)
                     /locus_tag="VGT44_07080"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEV2580602.1"
                     /translation="MDDLSVFTGNANRPLAAAICKHLNIPLGDADVFQFSNENIFVKI
                     NDNVRGRDVFVIQPFSFPVNRSIMELLIMIDALKRASAARITAVIPYYAYGRTDKKDQ
                     PRVPITARLIADCITVAGAHRVLTMDMHAGQIQGFFNIPVDELTAQILEARYFVEKGL
                     DDITVVAADEGFAKKARKLADRLNAPLAIVEKRRHGNNSIAEAMGIIGTVEGTHALIV
                     DDEIDTAGSLTQAVRVVHEQGARDIYCCATHGVLSGPALDRLRSAPIKEIVITDSIPL
                     ADQKRLPNMKVLSVAELFAGAISRIHDGRSVTELFW"
     gene            complement(2052..3017)
                     /locus_tag="VGT44_07085"
     CDS             complement(2052..3017)
                     /locus_tag="VGT44_07085"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580603.1"
                     /translation="MATMELTVLTEPIIDECECLPLIALSGGQAQQGDDYRPMTPVTP
                     ELGGYLREHLSAYFPRSSPISVLLLHISQLEQAHISPKFALLYKRRRFHAPESFMEQV
                     LANVRRSIRGSDQLIMHGGAGAAIIFPGVDREGAFSITERVYHSINLLQPDTVIPPLA
                     RETDIFVSMGSYPEPANTLEGLLHQTGYVVHRLTLRPAVTAQLRAHGAESEIEAESSV
                     RPATKTRHKGIPGEQPPPVNVSGIPYMQLPARLPARLRHLIPYHLARELRCAPVGRDH
                     NRLTIALAQPTDAQTIEQLKKSTGMVIYPVACEISALDALLANEW"
     gene            complement(3282..3971)
                     /locus_tag="VGT44_07090"
     CDS             complement(3282..3971)
                     /locus_tag="VGT44_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580604.1"
                     /translation="MVADDDRDMVDMLSYWLKGHGYDVVRAFDGEQAIKRWRETVPDL
                     VILDVQMPKLDGFEVCRQMRSETNSMVLILTAHDREDDEIRGLEMGADDFLRKPFSPR
                     QLLARIKAIMRRSNTSKGSSGSSSITVGPVTLDAMRHEVTRDGSKVRLTPTESRLLHL
                     LITHTGQVLTTDMIIERVWGYDEAGDSGLVKTHIRHLRQKVEPDANTPQYIMTVPGVG
                     YTFTAPVTIET"
     gene            4433..4837
                     /locus_tag="VGT44_07095"
     CDS             4433..4837
                     /locus_tag="VGT44_07095"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV2580605.1"
                     /translation="MVSRDELVRLAREAARHAHCPYSHFHVGAALVADGNIYTGANVE
                     NSSYGLSLCAERSALAAAISSGARTVTQVAVACIDVAPDAPLADRTPCGACRQWLADL
                     APRAAIYIDGDERDFTVTDLLPYAFGLEGPHE"
     gene            complement(4828..6597)
                     /locus_tag="VGT44_07100"
     CDS             complement(4828..6597)
                     /locus_tag="VGT44_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2580606.1"
                     /translation="MADLTGQQLGNYRIIRLIGRGGFADAYLGEHIYLRTQVAIKVLQ
                     AKVANDEDLEGFLKEARTIAHLIHPNIVRVTDFGVEAETPFLVMDYAPKGTLRQRHPK
                     GTIIPITTIIPYVRQVAMALQHAHDEKLVHRDIKPENMLLGRKDEVLLSDFGIALVSQ
                     SSRYESTQDVVGTVAYMSPEQIQGKPRPASDQYSLAVVVYEWLSGDRPFHGSFTELCT
                     QHMFASPPPLRERVPGISPDIEHVVTTALAKDPKQRFGSVLAFANALEQANQVSQPMP
                     SPVVKPAPEITPSAPPPTPAKEVAAWPPTQVASPPTPAKEVAAWPPTQVAPPPPPASA
                     AASSIPQAQIAPPPPAYSLRNLTKESPASYGANQWSPGMYQSARTDSDAKVRLWSFGK
                     ERLIAALLGIVIFGGGVYVFNLIGKQNSFIGPLFSPLIPSLGTVFGFFFNWYSILFSI
                     AFVIPLLLAVEYGPWVGTVTIVLGSLIGNYLVFHAFSTPWVYYLADALFAFVPGFALL
                     ATKGRYDNRKAIVIALVFSVVGILCFCIVTAIGDAVINHFLVSDDIGDFFGDLASFVI
                     CLALLPFLLMAFNAMMRRKNSYS"
     gene            complement(6651..6722)
                     /locus_tag="VGT44_07105"
     tRNA            complement(6651..6722)
                     /locus_tag="VGT44_07105"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6688..6690),aa:Thr,seq:tgt)
BASE COUNT         1642 a         1955 c         1896 g         1523 t
ORIGIN      
        1 aaatggaagg atcaaatgtg aacaaacaag atatagaaaa atatctgcgt atgctcggcc
       61 aggagctgct tcatcgccag gtaacgggcg aaattctcct tgtcggcgga gccgtgatgt
      121 tgcttaaagt gcagaatcga gaaaccacca aagatatcga tgcttacttt gttactggac
      181 aggctgagat catccgagaa gcagcgcagg ctatagctga tcgagaagga ctggcgcagg
      241 actggattaa cgatgctgca aaaggctttt tctacacgca gccttctacc gaacaatggg
      301 ctgagtatcc tggtttaaga gtctacatgc cttcgcttga ttatatattc gcaatgaaag
      361 tagttgcagg tcgccctcaa gacattgatg acatcaaggc attagcccag gaactgaacc
      421 tttcgaatgc acgagacgca ctcactatag tgaaagagta tatccctgag caattgctgg
      481 tcccgcgagt agaatacatg attgatgaga tattcgaata atttatacct gattatgaga
      541 atgacgagga gaagcaaaca atggcgcaga ctcaacccaa atggcagcca cagaccgctg
      601 cagaaacctt cgagcgcatc tgcaataata caacgccctg ggtagccatt ggcgatttcc
      661 tcgacgattg gcggcgcact cctgtcgaac acaggcacga actggctaca gagccaattg
      721 cttccgcgcc gacgccggaa ctacaacgtt gggcagcttt ctgcgccgca atggtggaat
      781 ggctctgttg gcaagaccat ttgccctttc catcatggac aagccaggca acctacaggc
      841 tgccacaacc ctggtttctt tataaaggtt ggaagcttcg cgcatggcaa ctggcaacga
      901 cgccgacacc cttcaagatg cgcaatatct tcgggggaga ccggatgctt gaccgggtct
      961 agcaacttct cgtcctacca gaacagctcc gtcaccgacc gcccatcatg tatcctgctg
     1021 atcgcccccg cgaacaactc cgcgacggac agcaccttca tgttgggcag gcgtttctga
     1081 tcggccaggg ggatcgagtc ggtaatcacg atctctttga tgggggcgga tctgaggcgg
     1141 tcgagggcgg gaccggagag gacgccgtgc gtggcgcagc agtaaatatc acgcgcgccc
     1201 tgctcgtgta cgacgcgcac ggcctgcgtg agcgaaccgg cggtatcgat ctcatcatcg
     1261 acgatcagcg cgtgcgtccc ttcaaccgtg ccgatgatgc ccatagcctc ggcgatgctg
     1321 ttgttgccat gcctgcgctt ctctacgatg gccagcgggg cattcaggcg gtcagccagt
     1381 ttgcgcgcct tctttgcaaa accttcgtcg gcggccacga cggtgatgtc gtcgagaccc
     1441 ttctcaacga aatagcgcgc ttcgagaatc tgagcggtca gttcatcgac ggggatattg
     1501 aagaaccctt gaatctggcc cgcgtgcata tccatcgtca gaacgcggtg cgcccccgcg
     1561 acggtgatgc aatccgctat caggcgcgcc gtgatgggaa cgcgcggctg gtctttctta
     1621 tcggtgcgcc cgtaggcata gtaggggatc acagcagtga tgcgcgcggc agaagcgcgt
     1681 ttgagtgcgt cgatcatgat cagcagttcc ataatcgagc gattgaccgg aaaactgaac
     1741 ggctgaatca caaagacatc gcgacctcgc acgttatcgt tgattttgac aaagatattt
     1801 tcgttgctga attgaaagac atccgcgtcg cccaatggga tattcagatg tttacagatg
     1861 gcagccgcta aggggcggtt cgcatttccg gtaaagacac tcaaatcgtc catcttgtcg
     1921 gttggttcct cctggctgct tgagtatatc acacggaatt tacttatgta aacaaagcgg
     1981 atggcaggag tttcagaggg cgaccgcaag ggtacgccct ggacattcgc cctggacgtt
     2041 cggatcactc tctaccattc attcgcaagt aaagcatcca gcgcgcttat ctcacaggcg
     2101 acagggtaaa tcaccatacc ggtcgatttc ttcaattgtt caattgtctg cgcgtcggta
     2161 ggctgcgcca gggcgatagt gaggcggttg tggtcgcgac cgaccggggc acagcgcagt
     2221 tcgcgtgcga ggtggtaggg gatcaggtgc cgcaagcgcg ccgggagacg ggcgggcaat
     2281 tgcatatagg gaatgccgct gacatttacc ggtggaggtt gctcgccagg gattccttta
     2341 tgtcgtgttt tagtagctgg acggacgctg ctttccgcct cgatctccga ctcagccccg
     2401 tgcgcgcgga gttgcgctgt caccgccggt cgcagggtca ggcggtggac aacgtaaccg
     2461 gtctgatgca gcaagccttc cagagtgttc gctggctctg gataagaacc catgctcaca
     2521 aagatatcgg tttcacgcgc aagtggtgga atcaccgtat caggttgcaa caggttgatg
     2581 ctatggtaca cacgctcggt aatactgaaa gcgccctccc ggtcaacgcc tgggaagatg
     2641 atcgccgcgc ccgcgccgcc atgcataatc aactgatcgc tgcctcggat gctgcgtcgc
     2701 acgttcgcca ggacttgttc catgaaactt tcaggggcat gaaaacgacg ccgtttgtac
     2761 aacagagcga attttggtga aatatgcgct tgttcgagct gcgaaatatg cagtaacaac
     2821 acactgatgg gcgatgagcg cgggaaatag gctgacaggt gctcccgcag gtaccccccc
     2881 agttccggtg tcaccggcgt catcgggcgg tagtcatcgc cttgctgcgc ctgtccgcca
     2941 ctcagggcga taagcggcag gcattcacat tcgtctatta tcggttcggt aagcactgtc
     3001 agttccatgg tcgccattat ttctcatcat aaacgcaatt tcaaaagaga agatttgctg
     3061 atttgactgt gattataccg gatacgagag aaggccatac tgcatgaagt gttacattgc
     3121 ggtaaagaaa aagcagagca ggttgagaaa atgacgtatc aaccatcgta ggaacaacac
     3181 cttccagggc gatggccagc atcgccccta ccctcgctcg atagcttctc tcgcggattg
     3241 gcagagggta ggggcgatgc tggccatcgc cctgggagca actatgtttc gatagtaacg
     3301 ggcgcggtga aggtataccc aacgcccgga acggtcatga tgtactgcgg tgtgttggca
     3361 tcaggctcca ctttctggcg caggtggcga atgtgggttt tgaccagccc ggaatcaccg
     3421 gcctcatcgt atccccagac gcgctcgata atcatatcgg tagtcaacac ctgcccggta
     3481 tgcgtgatga gcagatgcag caggcggctc tcggtcggcg tcaggcgcac cttcgagcca
     3541 tccctggtca cttcgtggcg cattgcatcc agtgtgaccg ggccgacagt gatagaagaa
     3601 gagccggaag aacctttgga agtattcgag cgacgcatga tcgccttgat acgagcgagc
     3661 aattgtcgcg gactaaacgg tttgcgcagg aaatcgtcgg cgcccatctc caatccacgt
     3721 atttcatcat cctcacgatc atgcgcggtc aggatcaaga ccattgagtt ggtttcgctc
     3781 cgcatctggc gacacacttc gaagccatcc agcttcggca tctgtacatc aagaatgacc
     3841 aggtcaggca ccgtttcgcg ccagcgtttg atggcctgtt cgccatcgaa ggcacgcacg
     3901 acatcatacc catgtccctt gagccagtag ctcagcatat caaccatgtc gcggtcatcg
     3961 tccgccacca tgattttcac tgattttcct cctacagacc tgcattactg aaccctctac
     4021 ccccggtaag acaggcccct actttgataa agttcgtctc attgctagta acgcgatgca
     4081 ttgatagcag taacatcatg gtacaatttg ggtcataacc agtcataacg tccgccattc
     4141 acgcctgttc gctactatta cctgtcaggg atatactata ctatcggcac gttctatccg
     4201 ccgtcgtagg gacgcgatga tgaacattcc caaccaaagt cgggaatgtc atcttgagtg
     4261 aagcgaagga tcagcgaagc atctaactgc ccatcgaccg agacccttcg cttcactcag
     4321 ggtgacaacc gcttaccgaa acctggttgg gaaacttcat catcgtgtcc tgatggatac
     4381 gctcgacagg tgaacgagga tgcgatgtta tcgaatataa gggaggaagc acatggtatc
     4441 tcgcgatgaa ttagtgcgcc tggcgcgcga agcagcacga cacgcccact gcccctactc
     4501 gcattttcac gttggcgcgg cgcttgtcgc ggatggcaac atctataccg gtgcgaatgt
     4561 ggagaactcg agctacggcc tgtcgctgtg cgccgagcgg tccgcgctgg ccgccgccat
     4621 cagcagcggc gcgagaacag tcacgcaggt cgcggtagcc tgcatcgatg ttgcccccga
     4681 cgcgccgctg gccgaccgca caccctgcgg cgcctgccgc caatggctgg ccgacctggc
     4741 cccgcgcgct gccatctata tcgacggcga cgaacgcgat ttcaccgtaa cagatctact
     4801 gccatatgcc tttgggctgg aaggacctca cgaataactg ttcttgcgcc gcatcatcgc
     4861 attaaatgcc ataagcagga agggaagcag cgccaggcag atgacgaacg aagccaggtc
     4921 gccgaaaaaa tcaccgatgt catcactaac taggaagtga ttgataactg catccccaat
     4981 cgcggtgacg atacaaaaac agaggatgcc aaccacactg aatacaagcg caatcacaat
     5041 cgccttgcga ttgtcgtagc gtccctttgt tgcaagcaac gcaaagccag gcacaaaagc
     5101 aaataaagca tcagctagat aataaaccca gggagtgcta aaggcatgga atacgaggta
     5161 attccctatc aacgaaccaa gcacgattgt tactgtgccg acccagggac cgtactccac
     5221 ggccagtagc agggggatca caaaggcaat actgaacaag atagaatacc agttaaagaa
     5281 aaacccaaaa actgtaccca acgacggaat caagggagag aatagcggac ctataaacga
     5341 gttctgtttt cctataagat taaaaacata gacgccgccg ccgaagatga cgatgcccag
     5401 cagcgcagct atcaggcgtt ctttcccaaa actccataac ctcaccttcg catcgctatc
     5461 tgtccgcgcg ctttgataca tgcctggcga ccattggttt gctccatacg aagcggggct
     5521 ttctttcgtc aggttcctca atgaatacgc gggcggcggc ggggctatct gcgcttgtgg
     5581 tattgatgat gccgctgcag aggcaggtgg aggaggaggc gctacctgcg ttggcggcca
     5641 tgccgctacc tctttggcgg gcgttggcgg cgatgctacc tgcgttggcg gccacgcggc
     5701 cacctctttg gcaggcgttg gcggcggcgc agatggcgtt atctcaggcg caggctttac
     5761 aaccggtgat ggcatcggtt gactgacctg atttgcctgt tccagcgcat ttgcgaatgc
     5821 cagcacactc ccaaaacgct gcttggggtc ctttgccagc gccgtcgtca caacatgctc
     5881 gatatccggc gaaatccccg gcacccgctc gcgcaacggt ggaggcgatg caaacatgtg
     5941 ctgcgtacac agctcggtaa acgatccgtg gaacgggcga tccccgctca gccactcgta
     6001 tacgaccacc gccagcgaat actgatcgct ggccgggcgc ggcttgccct gtatctgttc
     6061 gggcgacatg taagcgactg tgcctaccac atcttgtgta ctctcataac gcgaactctg
     6121 cgaaacgagt gcaatgccaa aatcgctgag cagcacttca tccttccgtc caagcagcat
     6181 attttccggt ttgatatcgc ggtgtaccag cttttcatca tgcgcatgtt gcaacgccat
     6241 agcgacctgc ctgacatacg gtataatcgt ggtgatgggg ataatggtcc ctttcgggtg
     6301 tcgctggcgc agtgtgccct tgggggcata gtccatcacc aggaatggcg tctcggcctc
     6361 cacgccaaaa tccgtcacac gcacaatatt cgggtggatc aggtgcgcaa tcgtgcgcgc
     6421 ctctttgagg aacccttcca ggtcttcgtc attcgctact ttcgcttgta gcactttgat
     6481 ggccacctgc gttctgaggt aaatatgctc gccgaggtag gcgtcggcaa acccgccgcg
     6541 cccaatcaag cgaataatgc gataattgcc aagctgctgc ccggtcaggt ctgccatctt
     6601 cccaactcct gagaaaaaca tcaatttagg cgagggatta taaaatttga agccggtggt
     6661 cgggatcgaa ccgacggcca gcggtttaca aaaccgctgc tctgccactg agctacaccg
     6721 gcccgattat agcaatttac aagcgttact ataccacgag tgtcaatatt cctagtctgg
     6781 ctgcattaaa gtcaaggatg gcagccggag tggacgggtg gagacccttg cggtcgccct
     6841 cgcaggcacg tcagcatagt ttttcttttc ctcacacccg ctgaaacaac cccggataat
     6901 ccactaccag cccatcggca tccacgcgaa ggtccgccgt atatccgctc gctagcccct
     6961 cgtatcgata caacccacca tgctcagttt tctccaggca ggtatagcgc tgctgc
//
LOCUS       DASZAK010000405         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_436686, whole genome shotgun sequence.
ACCESSION   DASZAK010000405 DASZAK010000000
VERSION     DASZAK010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436686"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1358
                     /locus_tag="VGT44_07110"
     CDS             <1..1358
                     /locus_tag="VGT44_07110"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEV2580607.1"
                     /translation="SLTSSCLGMPLMETPQQKAKRLRDRADQKKRSRFRLSRSSAIFR
                     LFAVLGPGLIAANAGNDAGGIATFSQAGASYGYGLLWALAISGICLAIVQEMCARMGT
                     VTGKGLADLIREQFGVRWAALAMLALLVANTGVTVSEFLGIAAAVQVLAGDPSTPLVY
                     VVVPLAGLILWWLVIKGSYRRVEKIFLLMSLGFLAYIPAAFAAHPAWSDVGRQVILPH
                     LSTDSGYLLTAVALVGTTISPYMQFFVQSSVADKGIHAGDYVYEQVDIYSGTTFAMLI
                     AGFVIVATGATLFVHNHPVQTALDAAQALQAFAGKYATLLFGIGLFGASLLAAAVLPL
                     STAYGICEAFGFERGVSRSFSEAPVFQSIFTGLIILGVLITLIPGLPIIQVLVVLQDL
                     NAAMLPILLVFIILLVNNRRLMGRHVNKLGFNLISWATVVVITILIVLLLLSTIFNIT
                     L"
     gene            1461..>2495
                     /locus_tag="VGT44_07115"
     CDS             1461..>2495
                     /locus_tag="VGT44_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2580608.1"
                     /translation="MSLNPDEFAEYREYPQGRSYEERPDYSDPAYYQQRSSATPRSAQ
                     PVAPEKVPDYAAPSMYSYDPGNMTAYKGPSSLEGYTADVGEEQREAAAHGASEQSKRR
                     KKLAGLGGIGASLIALAVKLKTLLVLLFDFKWLLAFSKIGLTSITVLISVGAYALIFG
                     WQFAIGLVALLFIHEMGHAVVMKVKGIPIGGMIFIPLLGAAVLMRQMPKNAKDEAEVG
                     IAGPIAGAIAASVCLLLAQANPDGIWAPLAYFGFFLNLFNLIPIVPFDGGRVLAAIDR
                     RLWIIGFLGLVAYEIWYFIQYQSINIWLLFFIVMAATQLWSRRKVADNPEAQAYYTVS
                     VGERIVLTLAY"
BASE COUNT          433 a          748 c          735 g          579 t
ORIGIN      
        1 agtctctcac ctcttcttgc ctgggcatgc cgctcatgga aacaccacag cagaaggcaa
       61 agcgtttgcg ggatcgtgct gatcagaaaa agcggtcgcg gtttcgtttg agccgttcgt
      121 cagcgatctt tcggctgttt gctgtgctgg ggccgggatt gatcgctgcc aacgcgggca
      181 atgacgcggg cggcatcgcc acgttttcgc aggcgggcgc gagttacggc tatggcttgc
      241 tatgggcgct ggccatctcc ggcatttgcc tggcgattgt gcaggagatg tgtgcgcgta
      301 tgggaacggt gacaggcaag gggctggccg accttattcg cgagcagttc ggtgtgcgtt
      361 gggcggctct ggcaatgctg gctttgctcg tggcgaatac cggcgtgacc gtctccgaat
      421 tcctcggcat cgccgccgct gtgcaggttc tcgcgggcga tccgtcaacg ccgctggtct
      481 atgtggtcgt tcctctggcg ggtctgatcc tgtggtggct ggtcatcaag gggtcatatc
      541 gccgtgtgga gaaaatcttc ctgctgatgt cgctcggctt tctggcctat atccccgccg
      601 cgttcgccgc tcatcccgcc tggagcgatg tcggacggca ggtcatcctc ccgcatctca
      661 gcacggatag cggctatttg ctgacggcgg tcgcgctcgt cggcactacc atcagcccgt
      721 acatgcagtt ctttgtgcaa tcctccgtcg ccgacaaagg cattcacgcg ggtgactatg
      781 tctacgaaca ggtcgatatc tacagcggca ccacgtttgc aatgctgatc gccggcttcg
      841 tcatcgttgc gaccggcgcg acgcttttcg tgcataacca cccggtacag accgcccttg
      901 atgccgcgca agcgttgcag gccttcgccg ggaagtatgc tacgctgctc ttcggcattg
      961 gcctgttcgg ggcatcgctg ctggccgccg ccgtgctgcc gctctcaacc gcgtatggca
     1021 tctgcgaagc ctttggcttc gagcgcggcg tctcgcgcag cttcagcgaa gcgcccgtct
     1081 ttcagagcat cttcaccggc ctcatcatcc tgggcgtgct gattacgctg atccccggcc
     1141 tacccatcat ccaggtactc gtggtgctgc aagacctcaa tgctgctatg ctgcccatcc
     1201 tgctcgtctt catcatcctg ctcgtcaata atcgccgcct gatgggccgc cacgttaata
     1261 aacttggctt caacctcatc tcctgggcaa cggtggtcgt gatcacgata ttgattgtgc
     1321 tgttgctgct ctccactata ttcaacatca ccttgtaggg gaatagcaga accttccagg
     1381 catgttgttc gtaaggagaa tgaaatggta tgatacaata ctcttagaga gctatatgcc
     1441 tgtgcagaac cgaggcgcgc atgtccctga accctgatga gttcgctgaa tatagagaat
     1501 atccgcaagg ccggtcatac gaagagcggc ccgattactc cgaccctgcc tactaccagc
     1561 agcggtcctc agcaacaccg cgctccgctc agcccgttgc gccggaaaaa gtccccgact
     1621 atgccgcgcc gtcgatgtat tcttacgatc cgggtaatat gaccgcctac aaggggccgt
     1681 cgtcgctgga aggctatact gccgatgtcg gcgaggagca gcgcgaagcc gccgcgcatg
     1741 gggcatcgga gcagagcaag cggcgcaaga aactggccgg gttaggcggc atcggcgctt
     1801 cgctgattgc gctggccgtc aagctcaaaa cgctgctggt actgctgttt gatttcaagt
     1861 ggctgctggc gttttccaag atcggactga ccagcatcac cgtgctgatc tcggtcgggg
     1921 cgtatgccct gattttcggc tggcagttcg cgatcggcct ggtagccctg ctcttcatcc
     1981 acgaaatggg tcacgcggtg gtgatgaagg tgaagggcat acctatcggc ggtatgatct
     2041 ttattccctt actcggcgcg gcggtgctga tgcgccagat gccgaagaac gcgaaggatg
     2101 aagcggaagt cggcatcgcg ggaccgattg ccggggcaat cgctgcctcg gtctgcctgc
     2161 tgctggccca ggcgaacccc gatggcatct gggcgccgct ggcctacttt ggcttcttcc
     2221 tcaacctgtt caacctgatc cccattgtcc ccttcgatgg cgggcgcgtc ctggcagcca
     2281 tcgaccgccg cttatggatc atcggcttcc ttggcctggt tgcctatgaa atctggtatt
     2341 tcatccagta ccagagcatc aacatctggc tgctgttctt tatcgttatg gcggccaccc
     2401 agctctggtc gcgccgcaaa gtcgctgaca acccggaggc acaggcatac tacacggtct
     2461 cggtgggcga gcgcattgtc ctgacgctgg cctat
//
LOCUS       DASZAK010000406         2173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_471571, whole genome shotgun sequence.
ACCESSION   DASZAK010000406 DASZAK010000000
VERSION     DASZAK010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471571"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VGT44_07120"
     CDS             <1..553
                     /locus_tag="VGT44_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEV2580609.1"
                     /translation="AQRRVEAGGAKITYTDLLVKVVAAALRSHPRANVSWQNGGIIQH
                     NEVHVGLAVAIDQGLVAPVIHHADRLSLRAIAARRQEMVALAQAGKLRPADLQGGTFT
                     ISNLGMYGVDAFNAIINQPQAAILAVGRIAERVVALHGQPSVQPVMVLTLSCDHRAID
                     GARGAQFLSTLAELVEEPLGLLA"
     gene            708..1988
                     /locus_tag="VGT44_07125"
     CDS             708..1988
                     /locus_tag="VGT44_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2580610.1"
                     /translation="MLGLRALWIKPVLKSVGVLIAALFVLSACGTTTTGGSTPTSTAS
                     CSGQTQPPIKIGISLSLSGDFSGDGQAFQQGYQLWADTVNKSGGVLCRQVQLDIVSDA
                     SNTTQVQTNYQKLISVNKDDIVFGPFSTLLTKPASVVANRFGYAMLEGAGGGPSVFTQ
                     GLNNLFDVSAPVANLLVSFTQYLLALPASQRPTTAAYATQNDPFTQPQVDTARALLEQ
                     GGIQTVSYQVYPSETTDFTPIAQKMIASKAQIIVTGTLLPDIVAYIQAFKQQHFNPTA
                     IIATAGPDQGSQFTNAIGGTTDAEGVFVPNGGWYPTLNAFQNAQMVSDYLAKYGGNAA
                     GISSDVAEAFSVGQVFQQAATKIGSIDNAKLIAELHSGDTFQSVQGPVKFNAQGENTL
                     ATGYLYQWQKGNLVSVYPANQASAPPEYPKPNWP"
BASE COUNT          460 a          632 c          656 g          425 t
ORIGIN      
        1 agcgcagcgg cgcgtcgagg ccgggggagc gaagatcacc tataccgatc tgctggtaaa
       61 ggtggtggct gccgcgctgc gcagccatcc ccgcgccaac gtttcctggc agaacggcgg
      121 cattatccag cacaacgaag tgcatgtcgg gctggctgtc gccatcgatc agggactggt
      181 agcgccggtc attcaccacg cggaccggct cagcctgcgc gcaatcgccg cgcggcgaca
      241 ggagatggtt gcgctggccc aggccggaaa gctgcgcccg gctgatctgc aaggcggaac
      301 gtttaccatc agtaacctgg ggatgtatgg tgttgacgcc ttcaatgcca tcatcaacca
      361 gccccaggcg gctatcctgg ccgtcgggcg catagcagag cgcgttgtgg cgctgcatgg
      421 ccagccctcg gtgcagccgg tgatggtcct cacgctgtcg tgcgaccatc gcgccatcga
      481 cggggccagg ggcgcgcaat tcctatcgac actggcggag ttggtggaag agccattagg
      541 acttttagct taactcaggt agttcgtgtg aaaggggtag ttgagcggac gtaggtagta
      601 gggggaagca actgtgcaat gttgtttcct gatctgctcg gtgagcaaga ataacaggga
      661 ggcgtgggag ctatcgtggc gcccactagt aacaaaggag gacagacatg ttaggacttc
      721 gcgctttatg gataaagccg gtcctgaaaa gcgtcggtgt tttgatcgct gcgctttttg
      781 tactcagcgc ctgcggcacg acgactacag gagggagtac tcctacttca acagcttcat
      841 gctcaggtca gactcaaccg cctatcaaaa tcggcatttc gctctcgctt tccggcgact
      901 tctccggcga tggtcaggca ttccagcaag gctatcagct ctgggccgac acggtaaata
      961 agagtggcgg cgtgctttgt cgccaggtgc agctggacat agtttctgac gccagtaaca
     1021 cgacgcaggt gcaaaccaac taccagaagt tgatcagcgt caacaaagac gacatcgtat
     1081 ttgggccatt ttcgacgctg ctgaccaagc cagcctcggt ggtggccaat cgctttgggt
     1141 acgcgatgct cgaaggtgcc ggtggtggtc cttccgtctt tacccagggg ttgaacaacc
     1201 tgtttgacgt gtcagcgcct gtcgccaacc tgctcgttag ctttacacag tatctgctgg
     1261 ctttgcccgc ctcacagcgg ccaacaacgg cagcctacgc cacgcagaat gacccgttta
     1321 cccagccaca ggtcgatact gcgcgggcgc tgctggagca agggggcatc cagacggtgt
     1381 cctaccaggt ctacccgtcg gaaacgaccg actttacgcc aattgcgcaa aagatgatcg
     1441 cttctaaagc gcagatcatc gtgaccggta cgctgctgcc cgacatcgtg gcctacatcc
     1501 aggcgttcaa gcagcagcat ttcaatccca ctgcgatcat cgcgacggcg gggcctgacc
     1561 aggggtcgca atttaccaat gccatcggtg gcacaacaga cgcggaaggc gtcttcgtgc
     1621 caaacggcgg ctggtaccca acgctcaatg ccttccagaa cgcccagatg gtcagtgatt
     1681 acctggcgaa gtacggtggc aacgccgccg gtatcagctc ggacgtggcc gaggcgtttt
     1741 ctgtcgggca ggtcttccag caggccgcga ccaaaatcgg cagcatcgat aacgcgaaac
     1801 tcattgccga acttcactct ggcgacacgt tccagagcgt gcaaggaccg gtgaagttca
     1861 acgctcaggg cgagaacacc ctggcgaccg gctacctgta ccagtggcag aagggcaacc
     1921 tggtctcggt ctacccggca aaccaggcgt cagctcctcc tgagtatccc aagccgaatt
     1981 ggccgtagca acgcgattca tcgcgtccgg cctggggcgg accccggtac ggacgcgatg
     2041 atggtgatta cattttacat cgggtacaca cgtaggtgcc gattgatcgc gcacaccgcc
     2101 gataaatcgg ccacgatggg catgtgagac aggcatccag gccgatcaat cggcggtgtg
     2161 cgcgatcaat cgg
//
LOCUS       DASZAK010000407         1747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_293293, whole genome shotgun sequence.
ACCESSION   DASZAK010000407 DASZAK010000000
VERSION     DASZAK010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293293"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..668)
                     /locus_tag="VGT44_07130"
     CDS             complement(<1..668)
                     /locus_tag="VGT44_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580611.1"
                     /translation="MEIGKIVKSYSHINYVCHIYGPREVEQEPTPADYAFGRFVRIAI
                     RSEKSDDHDAIIMAARGKSTEPQTYAVGVIYDTILVNPEFGSLGPRLSNETQVELFSP
                     DYISERAILIYVMVLGLTEQRIVPASKPEILSVMHGIPLPSLELGSDVEIMTDEEVRA
                     FHYFGDQRTGEQADYNGRQEKQQRQPYLHMGYIPHIIAQPNSLLPMVTLRIIEQLEQL
                     FPENL"
     gene            778..1305
                     /locus_tag="VGT44_07135"
     CDS             778..1305
                     /locus_tag="VGT44_07135"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV2580612.1"
                     /translation="MTQTGETGDARPKRLLSIGAGAVIVHDNRVLLVRNTYGVTKGRY
                     LLPAGRVNAGELPDQAAARETYEETGLRVRIEGLLGVRIWVMDDGEHNYFYMFRAKLL
                     SPLSDLRPNLDEIDDARFFTKQELEALSIDETWAGAVAIALKALEPGANTWPNFSDLS
                     SDSGVDTPDRWRIWM"
     gene            1446..>1747
                     /locus_tag="VGT44_07140"
     CDS             1446..>1747
                     /locus_tag="VGT44_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV2580613.1"
                     /translation="MPVLATKLYIPRHRPNVVSRPRLIERLNESLHRNLTLISAPAGF
                     GKTTLVSEWLAGGHRPAAWLSLDEGENDPARFLTYLVAALQTIAPDSGTGLLSLLQS"
BASE COUNT          385 a          503 c          474 g          385 t
ORIGIN      
        1 agattttcgg gaaaaagctg ctccaactgt tctatgatac gcagcgtcac cattggcagc
       61 aggctgttgg gctgcgcgat gatatgggga atgtagccca tgtgcaggta aggctgccgt
      121 tgttgtttct cttgtctccc attataatct gcctgctcgc ccgttcgctg atcgccgaaa
      181 tagtgaaagg cgcgcacctc ctcgtcggtc ataatctcga catcgctgcc gagttccagg
      241 gagggcagcg ggatgccatg catcacagag agtatctccg gcttgctcgc cggaacaatg
      301 cgttgctcgg ttaaccccag caccatcacg tagatcagga tcgctcgttc agaaatataa
      361 tcgggtgaga acagctctac ctgcgtttcg ttcgagagac gcggccccag cgagccaaat
      421 tctggattga ccaggatcgt atcgtaaatc acgcccaccg cgtaggtctg cggctccgtg
      481 cttttccccc gcgccgccat aatgatagcg tcatggtcgt cgctcttctc cgaacgtatg
      541 gcgatgcgca caaagcgccc aaaggcataa tccgccggag tcggttcctg ctccacctcg
      601 cgcggcccat aaatatgaca gacataattg atgtgcgaat atgatttgac aattttgccg
      661 atctccatcg cgtacccctc ttcaaattag aacaggtttt tgagtttttc gcttgcaggc
      721 attataacat aggttggtat gctatactat tgcattgaac gtttatgagg atcgtgtatg
      781 acacagacag gcgaaaccgg cgatgcgcgc cccaaacgct tgctcagcat cggcgcgggt
      841 gcggtgattg tgcatgacaa ccgcgtgctg ctggtacgca acacctatgg cgtcacaaaa
      901 ggtcgctacc tgctgccggc agggcgcgtc aacgccggtg aattgccgga ccaggcggcg
      961 gcgcgcgaga catacgagga gacaggtctg cgcgtacgca tagaaggctt attgggagtg
     1021 cgcatctggg tcatggatga tggcgagcat aactacttct acatgttccg cgcgaagctg
     1081 ctctcgcccc tctccgacct gcgtccaaac ctggacgaaa tcgacgacgc gcgtttcttt
     1141 acaaagcaag agctggaggc gctgagcatc gacgaaacct gggcaggcgc cgtcgccatc
     1201 gccctcaaag ctctcgaacc aggcgcaaac acctggccaa acttctccga cttgtccagc
     1261 gattcgggtg tggatacgcc agatcgttgg aggatatgga tgtagctgcc agggagaaaa
     1321 aatcgaaact ccgtcacttt caatgatgcc tacaaccttg tgcgctctca cacgtatctt
     1381 cattgaaaaa tcggcgcgcg aaagagagcg tgtacgccag gaaatctgac aattgaccca
     1441 tgtctatgcc agttctcgcc acaaaactgt atatcccgcg gcatcggccg aacgtggtca
     1501 gccgcccccg cctgatcgag cggctgaacg agagcctgca ccgcaacctg accctcatct
     1561 ctgctcctgc aggctttggg aaaaccacgc tggtcagcga atggcttgcc ggaggccatc
     1621 gacccgccgc ctggttgtcg ctggacgaag gcgagaacga ccccgcacgc tttctgacgt
     1681 acctcgtcgc tgctttgcaa acgattgcgc cggatagtgg taccgggttg ttgtctctgc
     1741 tccaatc
//
LOCUS       DASZAK010000408         5032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_10144, whole genome shotgun sequence.
ACCESSION   DASZAK010000408 DASZAK010000000
VERSION     DASZAK010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5032
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10144"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="VGT44_07145"
     CDS             <1..721
                     /locus_tag="VGT44_07145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2580614.1"
                     /translation="HFTQCGAAPVPPALARRFEERTRVTVMTSYGLTEAAPGTHSNPV
                     YDPRLIKVETIGLPIHDTKQKIVDIVTGQIELGVGEEGELIVQGPQVMLGYWKAPEAT
                     AEALRDGWLYTGDIGWRDEDGYVTITDRKKEMIKYKGFSVAPAQIEALLLEHPAVGDV
                     AVIAKPSEEAGEVPKAFVVLKAGYSNQSADELMTWVNDKLATFKNVREVEFIDAIPRN
                     PSGKILRRVLKEQERKKMGLS"
     gene            777..1691
                     /locus_tag="VGT44_07150"
     CDS             777..1691
                     /locus_tag="VGT44_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2580615.1"
                     /translation="MSKERHATMDVKSLVAIDVHTHAEVSSRIADDPMSQAMREASIQ
                     YFKNEGQRPTIAEVAAYYRERKMACVIFPVDMESASGLVRVPNEEVAEVAAENADVLI
                     PFASIDPAKGKMGAREARRLIEQYGVRGFKFHPSVQEFYPNDRNAYILYEVIAEAGLP
                     SVFHTGHTGIGAGMRGGGGVRLKYSNPIYLDDVAVDFPDMPIIMAHPSFPWQDEALSV
                     CLHKSQVYIDLSGWSPKYFPPQLIQYANTLLKHKILFGSDYPVITPDRWLADFEQIAI
                     RPEVRPLILKENAIKLLGLDTSDALSSV"
     gene            complement(1695..3020)
                     /locus_tag="VGT44_07155"
     CDS             complement(1695..3020)
                     /locus_tag="VGT44_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580616.1"
                     /translation="MSRPSGEPAISVGTLHPTVPERHLHGRRLVLARVSWIAVTLTLV
                     ILNLIALPDTYVAYFNITPQVLQELHRLGLSPTFYGILLTVENAPFQLVSLALGMLLF
                     LRRSDDRMVLFCAFALVTFGNALPLYNFNNGTLVPSLATNAVLRVVALVLFGTGEASL
                     VIFFYLFPSGHFAPRWTRWCALVVVAYWLAVVFFPTLPSNAGGPATYMIPLALLSAVV
                     AQVYRYGRVSTPRERQQTKWALFGFALAILLLVVNIPIGFLVPSSIQNDPVFGNLNPI
                     FGVALPLIPIFLAIAVLRSRLWDIDTLINKTLVYGLLTSLLGALYAGLIIGLENLVGL
                     FSRQAAGISPVLVVSTLVIYVLFNPVRKRIQNTIDRRFYRKKYDAVKALAAFSATLRN
                     EVDLATLSEHLQAVVQETVEPTHTSLWFVKSQKQPLAASPQFRSLSLSD"
     gene            3204..4691
                     /locus_tag="VGT44_07160"
     CDS             3204..4691
                     /locus_tag="VGT44_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEV2580617.1"
                     /translation="MDTDVRTRQGIVRGRIEEGVAVFKGIPYAAPPFGSNRFQPPRPA
                     EQWDGVREALSYGATVPKPPYFPPFDVLLPEPVVPGEDCLNLNIWTPDVGRSGLPVMV
                     WIHGGAFANGSNAILQYDGSRFARDGVVCVSINYRLGADGFLFLGDGISNLGLLDQIA
                     ALEWVQENIAAFSGDANNVTIFGESAGGMCVSTLLSMPRAKGLFRRVIAQSGAGHHVI
                     SPATGKRVGQYLAEKLGAEATRAAIAAESTDRLLQAQMELSGDAFGNPDPERWGEVAA
                     NLMPFEPVIDGDILPARPIDSIVGGAGAGVDVLIGSNTDEEMLFMLPNGSINHINEDM
                     LAGAVAAYGLPVTETIATYRAIRPGASAGELLEAILTDWFFRIPAIRLAEAHTKGSGA
                     TYMYEFAWRSPKFDGKLGACHALEVPFAFDTLDKEGFEEVMGSEPPQQLADAMHAAWV
                     AFAKGGDPGWPQFDLKRRATMRFDTKQELVEDPRAAERVLWEGVR"
BASE COUNT         1158 a         1298 c         1521 g         1055 t
ORIGIN      
        1 gcattttaca cagtgcggcg cggctccggt gcctcccgca ctggcccgcc gcttcgagga
       61 gcgcacgcgc gtgacggtta tgacatccta cggcctgacc gaggcggcgc ctggcacgca
      121 ctccaatccg gtgtacgatc cccggctcat caaggtcgag acgatcggcc tgccaattca
      181 cgatacgaaa cagaagatcg tcgatattgt gaccggccag attgaactgg gagttggcga
      241 agagggagaa ctgatcgtgc aggggccgca ggtgatgctg ggctattgga aggccccgga
      301 agcgaccgcc gaagcgctgc gggatggatg gctctatacg ggggatattg gctggcgcga
      361 tgaagacggg tatgtaacaa tcaccgaccg caaaaaagag atgatcaagt acaagggttt
      421 cagcgttgcc cctgcccaga ttgaagcgct gctgctcgag catcccgccg ttggcgacgt
      481 ggcagtcatc gctaagccga gtgaggaagc gggagaagtg cctaaagctt ttgtcgtact
      541 caaggcggga tactctaacc agagcgcgga tgaactgatg acttgggtca atgacaagct
      601 ggccaccttc aagaatgttc gcgaggttga attcattgat gccattccca ggaacccctc
      661 cgggaaaatc ttgcgccgcg tcctcaagga gcaggaacga aagaagatgg gcttgtctta
      721 aaccaatggg cgagagtaca ggtgttggta gccagagtca ccaaggaatt gagtagatga
      781 gcaaagaaag acacgcaact atggacgtga agagcctggt ggcaattgat gtgcatacac
      841 acgccgaggt gtcgagccgg attgcggatg atccaatgtc gcaagcgatg cgcgaggctt
      901 cgatacaata ctttaagaac gagggacagc ggcctaccat cgcggaggtt gcggcttact
      961 accgggagcg taagatggcc tgcgtgatct tccctgtaga catggagtcc gcgagcgggc
     1021 tggtccgggt tccgaacgag gaggtggccg aagttgctgc tgagaacgca gatgtattga
     1081 ttccatttgc cagcatcgac ccggcgaagg ggaagatggg ggcacgcgag gcgcgccgcc
     1141 ttatcgagca atatggggtg cgaggtttca aatttcaccc ctcggtgcag gaattctatc
     1201 cgaatgaccg gaacgcctac atcctgtacg aggtgatcgc tgaggccggt ctgccgagcg
     1261 tgtttcacac cgggcataca ggaatcggag caggcatgcg tggtgggggt ggcgtgcgtc
     1321 tcaagtactc gaatcccatc tacctggatg acgtcgcagt cgactttccc gatatgccaa
     1381 tcatcatggc ccacccctct tttccctggc aagacgaagc cctctcagta tgcctgcata
     1441 agtcgcaggt atatatcgac ctctccggtt ggtcccccaa atacttccct ccgcaactca
     1501 ttcagtacgc gaatacgctt ctgaagcaca agatactgtt tggcagcgat tacccggtga
     1561 ttacgcctga ccggtggctt gcagattttg agcagatcgc gatcaggccc gaggtgcggc
     1621 ccttgatcct caaggagaat gccatcaagc tactcggtct cgatacttca gatgcgttga
     1681 gttcagtcta gtggtcagtc cgataaggag agagatctaa attgcgggga ggctgccaat
     1741 ggttgcttct gcgattttac gaaccacaac gaggtgtgcg taggttccac ggtctcctgc
     1801 acgactgcct gcaagtgctc gctcaatgtt gccagatcca cttcgttgcg cagagtggcg
     1861 ctgaaggctg ccagtgcctt gacggcatca tatttctttc ggtagaagcg ccggtcaatg
     1921 gtattctgga tgcgtttgcg aacgggattg aagagtacgt aaatgactag agtagagaca
     1981 acgagtaccg gcgatatgcc cgctgcttgc cgactgaaca gcccaaccag gttctccagg
     2041 ccaatgatga ggccagcata gagggctccc agcaggctgg tgagcaggcc ataaaccagc
     2101 gtcttgttga tgagggtatc gatgtcccac agccgggagc gcaaaacggc aatggcgaga
     2161 aaaatgggga taagcgggag ggccacgccg aaaattggat tcagattccc aaacaccggg
     2221 tcattttgga tggatgacgg taccaggaaa ccgattggta tgttcacaac gagaagcaga
     2281 atagcgaggg caaagccaaa gagcgcccat ttggtctgct ggcgctccct gggcgtagaa
     2341 acgcgcccat agcgatagac ctgcgccacg acagcgctca gcaaggcgag agggatcatg
     2401 taagtcgccg gtccccccgc atttgagggt agagtaggaa agaagacgac tgccagccag
     2461 taggctacca ctactagcgc acaccagcgc gtccagcgcg gtgcaaaatg cccggaggga
     2521 aagagataga agaaaatgac gagtgatgcc tctccagttc caaacaagac gagggcgacg
     2581 acacgcaaaa cagcattggt cgccaggctc ggcacaagtg tcccgttgtt aaaattatac
     2641 aaagggagcg cattcccaaa ggtaacgagc gcgaacgcgc agaagagcac catgcgatca
     2701 tcggagcgac gcaagaagag cagcatcccc agagccaggc tcaccagttg aaacggggcg
     2761 ttctccacgg tcagcaggat gccgtagaaa gtgggggaga ggcccagccg gtgcagttct
     2821 tgcagcacct gtggggtgat gttgaagtac gcaacatagg tatctggcag cgcaatgaga
     2881 ttcaagatca ccagggtcaa ggtgacggcg atccagctaa cccgtgccag aactaaccgg
     2941 cgcccatgca agtgcctctc aggaacggtg ggatgaagag ttcccactga gatcgcgggt
     3001 tcgccagatg gccgggacat tttccagctc ctccttctta gctccaggcg tttcttctcc
     3061 ccttagaata ccatacgccc gttacgaaag cgctacgaat ccaggcagcg cggttttgtt
     3121 gtcccgtttg aatagctaag atgatatact ttctgtagag cttgtcagct tgtaatgttc
     3181 ctatttaatg aaggagatgc acgatggaca ccgacgtcag aacccggcag ggtatagtgc
     3241 gtggtaggat tgaagaggga gtggctgtat ttaagggaat cccatacgca gcgccgcctt
     3301 tcggctccaa ccgttttcag ccaccccggc cagccgagca gtgggacggg gtgcgtgaag
     3361 cgctcagtta cggcgcaaca gttcccaagc cgccgtattt cccacctttc gatgtgcttc
     3421 taccagagcc ggtggtgcca ggtgaggatt gcttgaacct caatatctgg actccagacg
     3481 tggggcgaag cgggctgccg gttatggtct ggatccatgg aggcgcattc gccaacggat
     3541 caaacgcaat attacagtat gacggcagtc gcttcgcacg cgatggagta gtgtgtgttt
     3601 cgattaacta tcgcctcgga gctgatgggt tcctttttct gggtgatggt atttccaacc
     3661 ttggtctact cgaccagatc gcagcactgg aatgggtgca ggagaacatt gccgctttca
     3721 gtggcgatgc taacaacgtg acgatcttcg gtgagtcggc tggtggaatg tgcgtttcca
     3781 cgttattatc tatgccgcgc gccaaaggcc tgttccgccg ggttatagcg cagagtggtg
     3841 cgggtcatca tgtcatctca cctgcgacag gaaagcgtgt gggccagtat ctcgctgaga
     3901 agcttggcgc cgaagcgacc cgagcggcta tcgcggcaga atccaccgat cgcctgctgc
     3961 aggcgcagat ggaactgagc ggcgatgctt tcgggaaccc tgacccggag cgctggggcg
     4021 aggtggcagc caacctgatg cctttcgagc cggtgatcga tggggatatt cttcccgcac
     4081 gaccgattga cagcattgtt ggcggggccg gggcaggagt tgatgtcttg atcggatcga
     4141 atactgacga agagatgttg ttcatgctac ccaatggctc gatcaatcat atcaacgagg
     4201 atatgctcgc cggggcggta gcagcatatg gactccccgt tacagagaca attgccacct
     4261 accgcgcaat acgtcctggt gccagcgcag gcgaactgct cgaggccatt ctcaccgact
     4321 ggtttttccg tatccccgcg atccgcctgg ccgaggcaca tacaaagggt agtggagcta
     4381 cctatatgta cgagtttgcc tggcgatcgc cgaagtttga tggaaagtta ggcgcgtgcc
     4441 atgccctgga ggtccctttc gctttcgata cgctcgacaa ggagggattc gaggaggtaa
     4501 tggggagcga acctccgcag cagcttgctg acgcaatgca tgccgcgtgg gttgcttttg
     4561 ccaaaggagg cgatcccggc tggccgcagt tcgatctaaa gcgaagggca acgatgcggt
     4621 tcgatacgaa acaggaactg gtggaggatc ctcgagcggc tgagcgggtt ttgtgggaag
     4681 gggtgcggta ggttctgatg ctgatgggct gggatgctgg catgaaggaa gcgtgaggcg
     4741 ttgaaacacc aggagatgtt cattccttcc tcgtgacgaa tacagcatac cagctcactt
     4801 gccacggtgg gacctatttc ctgttacaga ttttgcttct tcggcgctct tcccaggccc
     4861 ttgcgtacag tctctattcc tctttaccgc tcccctctcc ctggctgttc tgcagagcgg
     4921 tggaatattt atgcgctctc tgttgtaagt tcccaataat caggtttggt gcatagccga
     4981 aaatggcggc ggcaaacaag tactccatgg tgactgtacc actgaagatc gt
//
LOCUS       DASZAK010000409         4920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_186576, whole genome shotgun sequence.
ACCESSION   DASZAK010000409 DASZAK010000000
VERSION     DASZAK010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186576"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="VGT44_07165"
     CDS             <1..1050
                     /locus_tag="VGT44_07165"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580618.1"
                     /translation="IMIGGLVLLASTPLLVNAGSIAVFVAGLALLLVGKNILAPAYQG
                     LMPDRVPVEQRGEAAGYVGGMTILGNVVGLGLASWLLGKVNTNSITNTGLIRSNAGVY
                     YIITAVIVLTGLLVTIFGVREMPLLTRTRKLVRKERLLLRVAVRFARDWIDPWRSHNF
                     TMVFLARASLMLGLSLFMTYILYYFARVQHASNFVQAAGIIAMLALGGAVLSGVLSGV
                     LADRIRRRVPVVCAATLCMSAASLAFVLFPDNLMNWLWPLGVVFGLGYGAYSSVDWAL
                     SIDVLPSMEQAGKDMGVWNASATLPAVAAPLLGSVIISIASAAGQNALGYRLVFGAAA
                     FFLLLAAVALETTGA"
     gene            1123..3156
                     /locus_tag="VGT44_07170"
     CDS             1123..3156
                     /locus_tag="VGT44_07170"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV2580619.1"
                     /translation="MSRSITVEDLYKFKPTSHPRISPDGKRVAFVVTTIDERKHEYRS
                     SIWIVPSEGGEAQQFTSGTVNASSPAWSPDGRWLAFVSEREGEPAQSGDKDAKKQGKG
                     KAQIWLIPTDGGEAHQLTHMQHGASNPVWSPDSKRLLFNAPVGPADEETEDGKPLPKV
                     RVIDRLFYRLDGVGYIYERRSHLFLIDSSGGEAQQLTDGDWDDGDPAWSPDGSQIVFT
                     SSRTQDRWRLPTPDLYTLAISDGKAGDVRCLTDGSLACGSPTWSPDGITIAFLGTFKL
                     FSGGHTDVYTIPARAERGAATCLTRDFEGSFSDWTNSDVGDEHLAPQPAWSADGRTLF
                     ALASHRGATRIFALSLQGASKQPPTLTPGNIHVRDMSADRTTGTLALLIASPTRIQEI
                     YTRSTAASGELRRLTSFNEDFLGELALSTPEYLSYAGADGWPMDGWVMKPRDFDPAKK
                     YPLIVEIHGGPATQYGYGFFHEMQLLTAAGKVVLYTNPRGSIGYGRDFSLAVRGKWGE
                     KDSLDIMAGVDALIKQGYIDEKRLGVTGGSYGGFMTNWLIGHNDRFKAAVTDRSVTNL
                     ASSFGSSDSGWFFADEELETTPWDNLERYMHMSPIKYVKNMHTPLLIMHSEQDLRCNI
                     EEAEQLFTSLKYLGREVLFVRFEGQSHGLSRGGHPKLRKERLRHILGWFEKYL"
     gene            complement(3153..4283)
                     /locus_tag="VGT44_07175"
     CDS             complement(3153..4283)
                     /locus_tag="VGT44_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580620.1"
                     /translation="MNKKFTTALWFSVLLLLFAFLLAACGNTAASAQPGDVGVSQTTG
                     AQQTMTVSVTVAGVFQEYALPQTKSGMMRPAIDHEGRIWFGEMGHNYLAVFDPHVGTF
                     QQIVPPHGKFGVMGVEVAADDTIWFAEQYANYIGHYFPATHRFQIYPLPNILAPDPSN
                     PGKTVTLPAAPNDLTIDAGGNIWFTELNANDIARLDPHSGAIKQYALATSSKSQALNP
                     YGIAVDSRGRVWFTEVSSSSIGWLDPSTGAQHTYSFPGLSTGLMEIASDPRGLIWITS
                     FNAGLLLNLNPQTGAVTRYYASAPGGNAGGVYGLTITAGGEVWVTLAALNIIARLDVA
                     ADRFIYYPIPTSASLPLGIVSGPHGTFWFTEAGSDKIGMLQP"
     gene            complement(4289..4816)
                     /locus_tag="VGT44_07180"
     CDS             complement(4289..4816)
                     /locus_tag="VGT44_07180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2580621.1"
                     /translation="MITIRQACIEDAPGIAKVHVDSWRTTYKGIVPDDYLANLSYLHR
                     TTVWNDILRDTEKKYIVYVAENEAHELVGFVAGGPDREENPPYVAELYSIYILQAYQG
                     QGIGRQLVRVFAGQLLRVGMKSMLLWVFAQNPARRFYEALGGRYVKTALYEAGDVTIE
                     EVAYGWEDISVLAQD"
BASE COUNT          990 a         1464 c         1475 g          991 t
ORIGIN      
        1 atcatgattg gcggtctagt gcttctggca agtacgcccc tgctggtcaa tgccggaagt
       61 atcgccgtgt ttgtggcggg actggcgctg ctgctggtgg ggaaaaacat cctggcgccc
      121 gcctatcaag ggctgatgcc agatcgtgtg ccggttgagc agcgcggtga ggcggcgggt
      181 tatgtcggcg gcatgaccat cctgggtaat gtcgttgggc tgggactggc gtcatggctg
      241 ctcggcaagg tcaatacaaa ctcgataacg aacacagggc tgatccgctc gaatgctgga
      301 gtttattaca tcattaccgc cgttatcgtg ctgacgggct tattggtcac gattttcggt
      361 gtgcgcgaga tgcccctgct gacgcgcaca cgcaagctgg tgcgaaaaga gcgcctgctg
      421 ctgcgcgtgg ccgtccgctt cgcgcgcgac tggatagacc cctggcgcag ccacaacttc
      481 acgatggtct tcctggcacg cgcctcgctg atgctggggc tatcgttgtt tatgacctac
      541 atcctctatt actttgccag ggtccagcac gcctccaatt ttgtgcaggc ggctggcatc
      601 atcgctatgc tcgcattagg cggcgcggtg ctgagcggcg tgttgtcggg tgtgctggca
      661 gaccgcatcc ggcggcgtgt gccggtggta tgcgcggcga cgctgtgcat gagcgcggca
      721 tcgctggcgt ttgtgctgtt ccccgacaat ctgatgaact ggctctggcc gcttggggta
      781 gtcttcggcc tgggttacgg cgcgtacagc agcgtggact gggcgctctc catcgatgtg
      841 ctgccttcga tggaacaggc gggcaaagat atgggcgtct ggaacgcctc agcaacgctc
      901 ccagccgtcg ccgcgcccct gctcggcagc gtgattatca gcatcgcttc tgcggccggt
      961 cagaacgcgc tgggttatcg tctagtgttc ggcgcggcgg cgtttttcct gctgctcgcg
     1021 gcggtcgccc tggagactac tggtgcttga cgaacaaagt tgcgtgattt actataaaag
     1081 acattacaag gtgccatact acatctcgca aaggaaaagc tcatgtcacg ctcgattacc
     1141 gtcgaagacc tgtataagtt caagcccacc agccatccgc gcatttcgcc tgacggcaaa
     1201 cgggtggcat ttgttgtcac cacgatcgat gaacgcaagc acgagtatcg ctcgtccatc
     1261 tggattgtgc cgagcgaggg cggggaagcg cagcagttta cgtctgggac ggtgaacgcg
     1321 agcagcccgg cctggtcgcc ggatggacgc tggttggcgt tcgtctcgga gcgcgagggc
     1381 gaacccgctc agtcgggtga taaggacgcg aagaagcagg gcaagggcaa agcgcagatc
     1441 tggctgattc ccaccgacgg cggcgaggcg caccagctta cgcatatgca gcacggcgca
     1501 tcgaacccgg tctggtcgcc ggatagcaag cgcctgctct ttaatgcccc ggtcggcccg
     1561 gcggacgagg agaccgaaga tggcaaacca ctgccgaagg tgcgcgtgat tgaccggctc
     1621 ttctaccggc tcgacggcgt gggctatatc tacgagcggc gcagtcatct tttcctgatc
     1681 gatagcagtg gcggtgaggc gcagcagttg accgacgggg attgggacga cggcgatccg
     1741 gcctggtcgc ccgacggctc gcagattgtc ttcacctcca gccggacaca ggatcgctgg
     1801 cggctgccca cacccgacct ttatacgctg gcgatcagcg atggcaaggc gggggatgtg
     1861 cgctgcttga cggacggctc gcttgcctgc ggctcgccca catggtcgcc cgacggcatt
     1921 accatcgcct tcctgggaac gttcaagctg ttctcgggcg gccataccga tgtgtacacc
     1981 atcccggcca gggctgagcg cggcgcggcc acctgcctga cacgcgactt cgagggcagt
     2041 ttttctgact ggacgaacag cgatgtcggc gacgagcatc tcgcgcccca gcctgcctgg
     2101 tccgccgatg gccgcacact cttcgccctg gcaagccacc ggggcgcgac gcgcatcttc
     2161 gctctatcgt tgcaaggcgc gagcaagcag ccgcccacgc tcacgccagg aaatatccac
     2221 gtgcgcgaca tgagcgctga ccgcacaacc ggcactttag ccttgctgat cgccagtccc
     2281 acgcgcattc aggaaatcta cacacgctcg acagccgcgt cgggcgaatt gcgccgcctg
     2341 acgagtttca atgaagactt cctgggtgaa cttgcgctat caacgccaga atacctctcc
     2401 tacgccggag cggacggctg gccgatggac ggctgggtga tgaaaccacg cgacttcgac
     2461 ccggcgaaaa aatacccgct gatcgtggag attcatggcg gtcccgccac acagtacggc
     2521 tacggcttct tccacgaaat gcaactactg acggcggctg gcaaagtcgt gctgtatacc
     2581 aacccgcgcg gcagcatcgg ctatggccgc gatttctcgc tggccgtgcg cggcaagtgg
     2641 ggcgagaagg actcgctcga catcatggcc ggagtcgatg cgctcatcaa acagggctat
     2701 atcgacgaaa aacgcctggg tgtgacgggc ggcagctacg gcggtttcat gaccaactgg
     2761 ctgatcggac acaacgaccg tttcaaagcc gccgtgaccg atcgcagcgt caccaacctg
     2821 gcttccagtt tcggttcgag cgactcaggt tggtttttcg cagatgaaga actcgaaaca
     2881 acgccctggg acaacctgga acgctatatg cacatgtcgc ccatcaagta cgtcaaaaac
     2941 atgcacacgc ccctgctgat tatgcacagc gagcaagacc tgcgctgcaa tatcgaagag
     3001 gctgagcaac tcttcacctc gctgaagtat ctgggacgcg aggtgctgtt cgtgcgcttc
     3061 gagggccaga gtcatggcct gtcacgcggc ggccatccta aactgcgcaa ggaacgcctg
     3121 cggcatatac tgggctggtt cgagaagtat ctctaaggtt gcaacatacc aatcttgtcg
     3181 ctgcccgcct cggtgaacca gaaggtcccg tgcgggccgc tcacgatgcc gagcggcagg
     3241 ctggcgctgg tcggaatggg gtagtagatg aagcggtcag ccgcgacatc cagccgcgct
     3301 atgatgttga gggcagcgag cgtgacccag acctcgccac cggcggtaat ggtcaatcca
     3361 tacacacccc ccgcgttgcc tcccggcgcg gatgcatagt agcgcgtcac cgcgcccgtc
     3421 tgaggattca ggttaagcag caacccggca ttgaaggagg tgatccagat gaggccacgt
     3481 gggtcgctgg caatctccat caggccggta gatagacccg gaaagctata cgtatgctgc
     3541 gcgcccgtcg aggggtcaag ccagccaatc gagctgctgc tgacctcggt aaaccacacc
     3601 cttccgcgcg agtcaaccgc gataccatac gggttcagcg cctgggactt cgatgaagtc
     3661 gccagcgcat actgcttgat cgcgccactg tgcgggtcta accgcgcgat gtcgttggca
     3721 ttgagttcgg tgaaccagat gttaccgcca gcatcgatgg taagatcgtt tggagcggcg
     3781 ggcagcgtca ccgtcttgcc ggggttagat gggtcgggag cgaggatatt gggcaacggg
     3841 taaatctgga agcgatgcgt cgccgggaaa taatgaccga tgtagttcgc gtactgttcg
     3901 gcgaaccaga tggtatcatc ggcggccacc tccacgccca tgacaccgaa cttgccgtgc
     3961 ggcggcacga tctgctggaa cgtccctacg tgggggtcaa agacggccag gtaattatgc
     4021 cccatctcgc caaaccagat gcgtccctcg tgatcaatag cgggcctcat catgccactc
     4081 ttcgtctgcg gcagcgcata ttcctggaat acccccgcca ctgtgaccga cactgtcata
     4141 gtctgctgcg cgcctgtcgt ctgcgaaacg ccaacatcgc ccggttgcgc tgaggcagcg
     4201 gtattgccac aggcggcaag aaggaaggcg aagagcagca aaagaaccga aaaccataat
     4261 gcggtggtaa atttcttatt catagcaatc aatcctgcgc cagaacgctg atatcctccc
     4321 agccataggc tacctcttcg atagttacat ccccggcttc atatagcgcg gtcttcacgt
     4381 atcgtccgcc gagcgcttca tagaaacggc gcgcgggatt ctgggcgaat acccacagca
     4441 gcatagattt catgcccacc cgcaacaatt gcccggcgaa cacacgcaca agttggcggc
     4501 ctatgccctg tccctgatac gcttgcaaga tatagatgct gtaaagttcg gccacatatg
     4561 gcggattctc ttcccgatct ggcccgcctg ccacaaaacc aactaactcg tgtgcctcat
     4621 tttcagctac gtacacgatg tatttctttt cagtatctcg caaaatatcg ttccatacag
     4681 tagtacggtg caggtaactc agatttgcaa gatagtcgtc cgggacgatg cctttataag
     4741 tggtgcgcca gctatcgaca tgcaccttcg cgatgccggg cgcatcttcg atgcacgcct
     4801 gccgtatggt tatcatcgca cagactcccg tgtacgccat aatcgggatg cactgatgaa
     4861 gtttttatta ccaggggatg cgcctccgta ggtgccggtt caccgcgcac accgccgatt
//
LOCUS       DASZAK010000410         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_542451, whole genome shotgun sequence.
ACCESSION   DASZAK010000410 DASZAK010000000
VERSION     DASZAK010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542451"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..251
                     /locus_tag="VGT44_07185"
     tRNA            168..251
                     /locus_tag="VGT44_07185"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Ser,seq:tga)
     gene            451..1632
                     /locus_tag="VGT44_07190"
     CDS             451..1632
                     /locus_tag="VGT44_07190"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEV2580622.1"
                     /translation="MSFIGSLDQFDLSIILQKIEEYQKTGLLIVQQGETTIELSVQRG
                     CLVCIGPVKPGVTLGERFAQAGIIPPEACYSIELALGERYQSENDAARAFLDASYVTA
                     ERLSQWAINEASQVLHAILTWEDGDLYFEENQLPPANRFQVPISIISLLPPIAPTTVI
                     PPPLTPMATGTDISSPFSATPSVSAASLLTAFTVDSEPHTDKLTPASIAGAQQRVTDP
                     LAPVHIDASFIQPNMTLVPADLSAQRESNPQVTLTPEQWRLFTRANGETTLSLAAHEL
                     HMTPDQVRRVACELHALGLVTILTPAATPAFMTPTAFDMSAGMDAYFGLQSADVASIS
                     VMAKRHGRPPIETLSQWGNGGNGATFQLGSGWVVSPTGPQRVRQQYPEQDYDRAFAQA
                     S"
     gene            1742..2743
                     /locus_tag="VGT44_07195"
     CDS             1742..2743
                     /locus_tag="VGT44_07195"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEV2580623.1"
                     /translation="MDTMLADQLASTGMVLGSSRAMSEIAGQVLQVAAHPDTTVLLQG
                     ESGTGKDVIAHAIHTLSRRAVYAFVDINCAALPDTLLETELFGVESGAFTDAKASRDG
                     YLLRADGGTLFLDEIGSMPLHLQAKLLRFLETRRFRRVGSTKEVQVDLRIISATNVDL
                     AQAVMRRSFRDDLYYRLKVITLHLPPLRERPEDIEPLATHFLRQFAPQNRLMCINQPT
                     MQLLQRYAWPGNVRELRSMIQRGLILCDGEEIGPEHLPREIREAGTMSSAVQRLDALQ
                     EQIHLPPTGIDLPTLLTSIERRLVQEALEHCGGNQVHAAALLGISRDQLRYRMNQRP"
BASE COUNT          578 a          843 c          771 g          592 t
ORIGIN      
        1 cacgctgaac ggctccggcc tggccgttgg gcgcaccatg gcggcgctcc tcgaaactta
       61 ccagcaagcc gacggctctg tagttatccc aaaagtccta cggccatata tggctatgtc
      121 agccctcccc ttttcaaaga actagcgagt atgatatagt agccattgga gggatggcag
      181 agtggtctat tgcggctgtc ttgaaaacag ctgggttaat cgccccgggg gttcgaatcc
      241 ctctccctcc gccggggcca gcttctgtga ttgatcgaag aggtacttac gggggtgcgt
      301 atctcgccat ttacggtggc cgcgacaatc atttcgcgcg agtaccggaa tggtattgca
      361 gcgtttcgta gtgggctttt gcgcgggcat aggatgctgt tatggtgttc tatggcgcgt
      421 cgagagtcgt ttttggacgg acggtgttga gtgtcattca taggttcgtt agatcagttt
      481 gatctgtcaa ttattttgca gaagatcgag gaatatcaga aaactggatt actcatcgtt
      541 cagcagggcg aaacaaccat agaactctca gttcagcggg gctgtctggt gtgtatcggt
      601 ccggtcaagc caggcgtcac gctgggtgag cgctttgccc aggccggtat cataccgccc
      661 gaggcatgtt actcgataga actggcgctt ggagagcgct accagagcga aaacgacgcg
      721 gcacgggctt tccttgacgc ctcctacgta accgcggagc gtctgagcca gtgggccatt
      781 aacgaggctt cgcaggtcct tcatgcaata ctgacctggg aagacggcga cctctatttt
      841 gaagagaatc aacttccccc agcgaaccgt tttcaggtgc cgatctcgat catctccttg
      901 ctgccaccca tagcacccac aactgtaata ccaccgcctc tgacacctat ggccaccggg
      961 actgatattt cttctccatt ttcagccact ccttctgttt cggcagcctc gctccttact
     1021 gcattcacag ttgatagcga gcctcacacc gataagctga cacccgcctc aattgcaggg
     1081 gcccagcaga gagtcacaga cccgctcgcg cccgtccata tcgatgcatc ctttatccag
     1141 ccgaacatga cgctcgtgcc tgctgacctc tctgcccaac gcgaaagcaa tccacaggtg
     1201 acactgacgc ctgagcagtg gcgactcttc acccgcgcaa acggtgagac cacgttgtct
     1261 ctggccgccc acgaattaca catgacaccc gaccaggtgc gcagggttgc ctgcgaattg
     1321 cacgccctgg ggctggtgac aatcctcacg cccgccgcaa cgcccgcgtt tatgaccccc
     1381 actgctttcg atatgagcgc ggggatggat gcgtactttg gcctccaatc ggctgatgtc
     1441 gcatctatct cggtcatggc caaacggcat gggagaccac cgattgaaac actctcgcag
     1501 tggggcaatg ggggcaatgg cgccaccttc cagctgggca gcggttgggt tgtgtcgcca
     1561 accggaccgc agcgcgtccg gcagcagtac ccggagcagg attacgacag ggcattcgcc
     1621 caggccagct aaacacaagc attgcaagca agaagggccg gtcggccggt ccccttcttt
     1681 caggggatat gtcgcgatgg gatggagtgc ttttccttcg acagcatcaa gccagatatc
     1741 aatggatacc atgctcgcgg atcagcttgc cagcaccggg atggtgctgg ggagcagtcg
     1801 ggcgatgtcc gagatcgccg gccaggtcct tcaggtggcg gcccatcctg ataccactgt
     1861 gctgctgcaa ggcgaaagcg gcaccggcaa agatgtcatc gcccatgcca tccataccct
     1921 gagccggcgc gctgtgtacg ccttcgtcga tatcaactgc gccgcgctgc ccgatacgct
     1981 gctcgaaaca gaactcttcg gcgtcgagtc cggcgccttc accgatgcca aagcatcgcg
     2041 ggatggctac ctcttgcggg cggatggcgg cacactattc ctcgatgaaa tcggctcgat
     2101 gccgctgcac ttacaggcca aattgctgcg cttcctcgaa actcgccgct tccgccgtgt
     2161 aggcagcacc aaagaggtgc aggtcgatct ccgtatcatc tccgccacta atgtcgatct
     2221 tgcgcaggcg gtgatgcgcc gatcatttcg ggacgatctc tactaccggc tgaaagtgat
     2281 cacgctgcac ctgcctcctc tgcgcgaacg accggaggat atcgaaccat tagcgaccca
     2341 tttcttgcgc cagttcgccc cacagaaccg gttgatgtgc atcaaccagc cgaccatgca
     2401 gttgctgcaa cgctatgcct ggccgggcaa tgtgcgtgaa ctacgcagta tgatccagcg
     2461 cggcctgatt ctgtgcgatg gagaggaaat cggtcccgaa cacctcccac gcgaaattcg
     2521 agaggcgggt acgatgagta gcgcggtgca gcggctcgac gcacttcagg aacaaatcca
     2581 cctgcccccc accggcatcg acctcccaac gttactcacc agcatcgagc gtcgcctggt
     2641 gcaggaagcc cttgagcact gcggcggcaa ccaggttcac gcggcagccc tgctgggcat
     2701 ctcacgcgac cagttgcgct accgcatgaa tcagcgtcct tgattctaaa ttgtgatgat
     2761 ggctaatgca gtcaactttc gtag
//
LOCUS       DASZAK010000411         2094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_152421, whole genome shotgun sequence.
ACCESSION   DASZAK010000411 DASZAK010000000
VERSION     DASZAK010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152421"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VGT44_07200"
     CDS             <1..325
                     /locus_tag="VGT44_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2580624.1"
                     /translation="DVWGNCIVATIAEDTPTLRAVNPLTWIKKTDYLELEFRPSLSSF
                     ATQRAELLAVLEPLPPEGWERSATVTGAGKILERTVLFYGQWLARHERQHVKQVEHIV
                     STMHI"
     gene            complement(532..1503)
                     /locus_tag="VGT44_07205"
     CDS             complement(532..1503)
                     /locus_tag="VGT44_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEV2580625.1"
                     /translation="MTNMTIPHFRDVLQAQRRIRPYLARTPLHSYPAINELVGTTLFI
                     KHENYQPVGAFKVRGGINLVSQMGPDERQRGIISASTGNHGQSVAYAARLFGVKARIV
                     VPEKANPGKVAAMQGMGAEVIAWGANFDEAREHCEALAQEYGYRYIHSGNEPHLIAGV
                     ATEALEMLEDEPELEVIIVPIGGGSGAAGTCLTAHALNPAIRVIGVQSAAAPAAYESW
                     RQRRLTTAPNHTIAEGLATGTAFALPQSILWELLHDFILLPDEEILRAQLWLIERAHT
                     LAEPAGAASLAAAYHLRKELSGKKVGIICSGGNTSLEHLRLALAQPT"
     gene            complement(1558..>2094)
                     /locus_tag="VGT44_07210"
     CDS             complement(1558..>2094)
                     /locus_tag="VGT44_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2580626.1"
                     /translation="NTNNRYVRMMNNDSKPHFPERLQNSRLQRGWKQARLAEELGVKL
                     RTLISWETGERVPTVGIVVRLGALLETDLLTPYIEDDLRRRYGQEAIRDFASVFPNTA
                     KLNSEEETHTQEKQSLQERQNETDSIPSDMEQPQHDQSQMANDANSLQSLFHIFEILQ
                     AQPALIPVALDFLEEMAD"
BASE COUNT          431 a          573 c          614 g          476 t
ORIGIN      
        1 tgacgtctgg ggcaactgca tcgtggcgac gatcgccgag gacacgccga cgctgcgggc
       61 tgtcaatccc ctcacctgga tcaagaagac ggactatctc gaactggaat tccggccttc
      121 gctcagctcc tttgccacgc agcgtgccga gctcttggca gtcctggaac cgttgccacc
      181 cgaaggctgg gaacgatcgg cgacagtgac gggggcagga aagatcctcg agcgaaccgt
      241 cctgttctat ggtcaatggc tggctcgcca cgaacggcag cacgtcaagc aagtcgaaca
      301 catcgtcagc acaatgcaca tatgagcctc ggccacccgc ttccgcaggc gacactatcg
      361 tgaggtgcca cccaggtcca cgaactccac cgtatcgccg gtctcgatgc cgaggagaaa
      421 ggggagtggt cgtcattgtt gtttgtcagt taaaaaggct ctggcacacg cttggtgaat
      481 ggggaaatgc attccaatgc agtactttct ggcctcaaga aagaccttct gttaggttgg
      541 ctgggcgagg gcgaggcgca ggtgttccag ggaggtattt cccccggagc agatgatgcc
      601 caccttcttg ccgctcagct cctttcgcag gtggtaggct gcggcaaggg aggctgcccc
      661 cgcaggctct gcaagggtgt gggctcgttc gatcagccac aactgtgccc gcaagatctc
      721 ttcgtcgggg agtaggatga agtcgtgcaa cagctcccag agaatgctct gcgggagggc
      781 gaaggcagtt ccggtggcta atccctctgc gatggtgtgg ttaggggctg ttgtcaggcg
      841 gcgctggcgc catgactcgt aggcggcagg ggcggctgcg gactgcacgc caatgacacg
      901 gatggctgga ttgagagcgt gggcggtgag gcaggtccct gccgcgccgc tgcctccccc
      961 aatggggacg atgatgacct ccagttctgg ttcgtcttcc agcatttcca gcgcctcggt
     1021 ggcaacacca gcaatgaggt gtggctcgtt gccggagtgg atgtagcggt acccatactc
     1081 ttgcgccagg gcttcgcagt gctcgcgagc ctcgtcgaag tttgcgcccc atgcaatcac
     1141 ctcggccccc atcccctgca tggctgcaac cttgccagga ttggccttct ctggcaccac
     1201 gatgcgagcc ttgacgccga agagacgcgc ggcataggcg actgactgac catgattgcc
     1261 tgtggaggcc gagataatgc cacgctggcg ttcgtcgggt cccatctgtg agacgaggtt
     1321 gatcccgccg cgcaccttga aggcccctac cggctggtag ttctcgtgtt ttataaaaag
     1381 cgttgtcccg actaattcgt tgatggcagg gtagctatgg agaggagtac gtgccaaata
     1441 ggggcgaatg cggcgttgcg cctgaaggac atcccggaaa tgaggaatag tcatgtttgt
     1501 catatgtaag cctttccaag atactaactc ataaaaagtc acatatctgc aataacccta
     1561 atcagccatt tcttcaagaa aatcaagtgc gacgggaatc aaagctggtt gtgcttgcag
     1621 aatctcaaag atatggaaga gagactgcaa cgagttcgcg tcattggcca tctggctttg
     1681 atcgtgttga ggttgttcca tgtcagaggg aatagaatcc gtctcgtttt gacgctcctg
     1741 aagcgattgt ttctcctgag tgtgtgtctc ttcctcacta ttcaacttcg cggtatttgg
     1801 aaagacactt gcgaaatccc ttattgcttc ttgcccatag cgacgcctca aatcatcctc
     1861 aatatagggg gtgagcaggt ctgtttcgag caatgctcca agacgtacca caatgccgac
     1921 cgtaggaacg cgttcgcccg tctcccaact gataagcgta cgcagcttta cgcctaattc
     1981 ctcggccagc cgcgcttgtt tccaccctcg ttgtaagcgg ctgttctgta agcgttcagg
     2041 gaagtgaggc ttgctatcgt tgttcatcat gcgaacgtac ctattgttgg tgtt
//
LOCUS       DASZAK010000412         3135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_223354, whole genome shotgun sequence.
ACCESSION   DASZAK010000412 DASZAK010000000
VERSION     DASZAK010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3135
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223354"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..933)
                     /locus_tag="VGT44_07215"
     CDS             complement(7..933)
                     /locus_tag="VGT44_07215"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV2580627.1"
                     /translation="MTLPRLDSLTIRQLRVFCTVARLLSYTRAAEELGCQQPTASALV
                     AELERATQLTLLEQRGKHLTLTDEGRELYAHAQQVVAAADEAWWAMAERRGSVTMVSR
                     PLRVAADTTVGTYVLPRLLGDFHQQHPKVALQLNIANRAAVRACLLAGEADLVIAGRP
                     PEVDGLVAEPFLANPLVAVASPEHVLVGQNQVSLARLAEEVFLLREEGSGTRAAVEEL
                     FEVAGMPLKVGMVLGHVEAIKRAVAAGLGVSVLSALAVKREVQYRTLAILDVEQFPIQ
                     RRWYIARFAERPLIASADVFIKFLHEYKQKSV"
     gene            1101..2051
                     /locus_tag="VGT44_07220"
     CDS             1101..2051
                     /locus_tag="VGT44_07220"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF005655.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV2580628.1"
                     /translation="MAVTFGTNPRPIILGIVGDSASGKTTLSRGIVQILGSEHVSVLC
                     TDDYHRFNRKQRREMGITPLNPACNYLDIMAQHLQLLRAGEPILKPHYDHKWGDFGPP
                     QYIVPQPFLIVEGLLGMYWQEMRNAYSVRVFLNPPEEQRRRWKIRRDTTQRGYTEAEV
                     LRELDIREPDSEAYIRPQRHHADVVVSFFPGTSSRTKLSDDAHLDVRLTLYGTLPHPD
                     LSEVIDEDRSGCVRATLGREGGRAVEYLEIDGAIPDEAAAALERCILDHLDACGAASL
                     ADLGLYSDPQVRHSHPLALSQLLLVYHLLRAKQREEVEAC"
     gene            2476..>3135
                     /locus_tag="VGT44_07225"
     CDS             2476..>3135
                     /locus_tag="VGT44_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2580629.1"
                     /translation="MEEDEPRDEFLIDAIAGGSMWALERLYHRYSRRFYGLAYRVTAD
                     HMVAEELVQDAFIAVWQSATSYAPQAGPVSAWLFSIIYHHTINYLRGVRRRSALQQVP
                     LQEIEAYERFALSDVWEQAWSSEQNAELHACLIRLPTEQQEAIELAYFGGLTHGEIAR
                     HCQIPLGTVKARIRLGVHHLRQTLDQRGNGENISPRATNNGRARPGRAESVVVQVTEN
                     GC"
BASE COUNT          689 a          891 c          885 g          670 t
ORIGIN      
        1 attaccttat acgctctttt gtttgtactc gtgtaggaac ttgatgaaca cgtcggcgct
       61 ggcaatcaag ggacgttcag caaagcgcgc gatataccag cgccgctgga tcggaaactg
      121 ctccacatct agaatcgcca gtgtgcgata ctgcacttcg cgtttcacgg ccagagctga
      181 gagcacgctc actcccaacc cggccgccac ggcccgcttg atggcttcga catgcccaag
      241 caccatgcca actttgagtg gcattccggc gacctcaaac agttcttcta ccgcggcccg
      301 tgttcctgag ccctcctcgc gcagcaggaa cacttcttcg gccaggcgag ccagcgatac
      361 ctggttctgc ccaaccagta catgctctgg ggaggcaacc gccactaacg ggttcgccag
      421 gaagggttcg gccaccagtc catcaacctc tggaggtcgg ccagcgataa ccaggtccgc
      481 ctcccctgcc aggagacagg cacgcacagc agcgcggttg gcaatattca gttggagtgc
      541 cacttttgga tgttgttggt gaaaatcacc caacaggcgt ggaaggacat aggtccctac
      601 tgtcgtatcg gctgcaaccc ggagcggcct gctcaccatc gtcacgctcc cgcgtctctc
      661 cgccatagcc caccatgctt catccgcagc cgctactacc tgctgcgcat gggcgtacag
      721 ctcgcgtcct tcatccgtca gcgtgaggtg tttgccccgc tgctccagca atgtcaactg
      781 tgttgctcgt tccagttccg ccaccagcgc cgaggctgtg ggctgctggc agccaagttc
      841 ctcagcagct cgtgtatagc tcaagagtcg tgcgactgtg caaaacaccc gcagttgacg
      901 gatcgtgaga ctgtcgaggc gtggcaacgt catgtataac gtccaatcta tgtatagtat
      961 agcgaattgc gccttgttct tatagatgat accacgtatc attctatcat agcgcagccc
     1021 agactagtgg caggttttga gtaacaatcg caccgggtga tcgaccaggg acatgcggca
     1081 taaggaggtt ggagaacgat atggctgtaa catttggaac gaacccacgc cctatcattt
     1141 tggggatcgt gggagacagt gcctctggta agaccacatt gagtcgaggc atagtccaga
     1201 ttttgggctc tgagcatgtc tcggtgctct gcacagacga ctaccaccgc tttaatcgca
     1261 agcagcgcag ggagatgggc atcacgccct tgaaccccgc gtgtaactat cttgatataa
     1321 tggcgcagca tctgcaactg ctgcgcgctg gtgagccaat tctgaagcca cactacgacc
     1381 acaaatgggg cgacttcggc ccgccacaat acatcgtccc acagcccttt ctgattgtcg
     1441 agggactgct cggcatgtac tggcaggaga tgcgcaacgc ctacagcgtg cgtgtctttc
     1501 tcaatccacc cgaggagcag aggcggcgct ggaagatccg gcgcgatacg acgcagcgcg
     1561 ggtacacaga agcagaagtg ctgcgcgaac tggacatccg cgagccagat tcggaggcct
     1621 acatccggcc acagcggcat cacgcggatg tcgtggtgtc cttctttcct ggcacatcgt
     1681 cccggacgaa gcttagtgat gacgcacatt tagatgtgcg tttgaccttg tatggcacgc
     1741 tgcctcaccc ggatctgagc gaggttatcg acgaggaccg gtcgggctgt gtacgtgcga
     1801 cgctgggacg tgaaggtggc cgggccgtcg agtacctgga gatcgatggc gctattcccg
     1861 acgaagcggc tgctgccctc gagcgatgta ttctcgatca cctggacgcc tgcggagccg
     1921 cctcgctggc agaccttggg ctctattccg atccccaggt ccggcatagc catccgctgg
     1981 cgctatccca gttgctgctg gtgtatcatc tgctccgcgc aaagcaacgc gaggaggtcg
     2041 aggcatgttg aatagcctta cttagtacac ccggaagtcg agcgcgagca catcgagcga
     2101 gcattacgta cggccagctt cgcaccagaa gagctgccgc tctagcgcgg cggttatcta
     2161 tgctagttcg gaataactga ttgagtcgaa aagtccaact caatcgtgct aaataaaatg
     2221 gaaaaggagc aaagccatca acataaaatg cggtaagacc cccctttgta cgaggcaaat
     2281 ccttttgcag cgactcccgt catcgggcgc ttagaggcgc ctggatcggc ggtgcagaag
     2341 cgaagtcctg gtgaatgctt gtatctcacc ggtacaagca gcgtgattct tgattggttc
     2401 tttaccacgg cattgtcaac ccggcaggcc tggtgggctt gagaggtaca ttcctcctcg
     2461 aaatgaagga cgaatatgga agaagacgag ccgcgtgacg agtttctgat tgatgccatc
     2521 gctggtggat ccatgtgggc gctggaacgt ctctatcacc gctacagcag gcgattctat
     2581 gggctggcct atcgtgtgac cgctgatcat atggtcgccg aagaattagt gcaagatgcc
     2641 ttcatcgccg tctggcaaag cgccacttcg tacgctccgc aagcgggccc agttagcgcc
     2701 tggctcttct cgattatcta ccatcacact attaattatt tacgcggcgt gcgccgtcgt
     2761 tccgcattgc agcaggtgcc cttgcaggag attgaagcct atgagcgctt tgccttatcg
     2821 gatgtatggg agcaggcatg gagttctgaa cagaacgcag agctacacgc gtgtttgata
     2881 cgattgccca ccgagcaaca ggaggcaatt gaactcgcat attttggggg attgacacac
     2941 ggagagatag ctcggcactg tcagattccc ctcggcaccg tcaaggcgcg cattcgcctg
     3001 ggggtacatc acctcaggca aacacttgac caacgaggaa atggcgagaa tatatctccc
     3061 cgtgctacga acaacgggag agccaggcca gggcgggcag aaagtgtggt tgttcaggta
     3121 acggagaacg gatgt
//
LOCUS       DASZAK010000413         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_542529, whole genome shotgun sequence.
ACCESSION   DASZAK010000413 DASZAK010000000
VERSION     DASZAK010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542529"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="VGT44_07230"
     CDS             <1..913
                     /locus_tag="VGT44_07230"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580630.1"
                     /translation="ARLAAIYASVFLLSGISRFFTPARAGVLQVVVAPQQQAQAASIG
                     QVTFAIAIIVGPAIASPLYFVVGPVVAILINAVSFLVSALFLLAIRVSRKDLRPYVQP
                     EKGGASAVVSELYAGLRLIATTRVLLMVIILALIAMLGAGALNALDIIFVSQRLHVSV
                     SLYGPLSAVAGLGTLVGAVLAGVLASRIKPRVMLAGCVLLLGMGIVVYSFQSWFLAAL
                     IINFFAMIPQGGIDVGVAPLLMNSTPHEMMGRVQSFLEMTMYGVSLLSVALAGYFGQF
                     IPVYVIFAICGGLIALAGVFGWFAIPS"
     gene            1108..2229
                     /locus_tag="VGT44_07235"
     CDS             1108..2229
                     /locus_tag="VGT44_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV2580631.1"
                     /translation="MVDVRLQRMAQVLVRYCVGAREGDRLAINSAPAAQALLLEVYRE
                     ALRVGTHPELFLALPGQAEIYYQEASDEQLAYIPAARRLMIEDNEALIVIDAQENTKS
                     LNGINPERFAIARKAGEELNLKVLQRTNERDPYAPGSLRWNRTLFPTDAYAQDAEMSL
                     AEFENFVYHACFLDDDDPVARWQELSAQQERLVQWLHGRHTVHLRGQDTDLTLSIEGR
                     TFINDDGHYNLPGGEFFTGPVEQSANGFIRYSFPCSFQGRSVEDVRLRFENGVVVEAR
                     AGQGQEYLDRMLAMDEGARRIGEFAFGNNRNVDRCTKHTLFDEKMGGTVHLALGASIP
                     QTGGVNVSALHWDMVCDLRHGSEIRVDDELFSKDGRFVV"
BASE COUNT          398 a          584 c          740 g          544 t
ORIGIN      
        1 ggcacgcctg gctgctatct atgccagcgt gttcttgctc tccggcattt cgcgcttctt
       61 cacgccggcc agggcgggtg tgttgcaggt tgtcgtcgcc ccacagcagc aggcgcaggc
      121 cgcttcgatt gggcaggtca ctttcgctat cgccatcatc gtcggaccgg ctatcgcctc
      181 gccgctctac ttcgttgtgg ggccggttgt ggcgatactg atcaatgcgg tctcgtttct
      241 ggtctccgcg ctgttcttgc tggcaatccg tgtctcgcgc aaggacctgc ggccctatgt
      301 ccaacccgag aaaggcgggg cgagcgcggt cgtttccgag ctgtacgccg gtctgcggct
      361 gatcgctacc acgcgcgtcc tgttgatggt gattatcctg gccttgatcg ccatgctggg
      421 cgcgggcgcg ctcaatgccc tggatattat cttcgttagc cagcgcctgc atgtcagtgt
      481 cagcctgtat ggtcccctgt cggcggtggc gggtttgggg acgctggtcg gcgcggtcct
      541 ggcgggtgtg ctggcatcgc gcatcaagcc gagggtaatg ctggcgggct gtgtgctgtt
      601 gctgggtatg gggattgtgg tgtactcttt ccagagctgg ttcctcgcgg cgctcatcat
      661 caactttttt gctatgatcc cgcagggtgg catcgacgtg ggcgttgcac ccctgcttat
      721 gaactcgacg ccgcatgaga tgatggggcg cgtgcaatcg ttcctggaaa tgacgatgta
      781 cggcgtgagt ctgctctcgg tggcattagc cggctatttc ggccagttta tacctgtata
      841 tgtcattttt gccatttgcg gcggcttgat cgcgctggcc ggcgttttcg gctggtttgc
      901 aataccgtcg tagggatgtg atgacgaagt ttaacgacca aggccggtca gaggttgtca
      961 ccctgagcgc agcgaagggt ttgatgcgct tgacaccgag atccttcgct gcgctcagga
     1021 tgacaggcgt aggtgccaat cctggttggg aatgttcatt atcgcgtccc tacgacaaat
     1081 aaaagaaagg agtaaggagc tactactatg gttgatgtac gattacaacg tatggcgcag
     1141 gttctggtgc ggtattgcgt tggcgcacgt gagggcgatc gtttggcgat caattcggcg
     1201 ccggcggcgc aagcgctttt gttggaggtc tatcgcgagg cattgcgcgt tgggacgcat
     1261 cctgagctgt ttctggcgct gccaggacag gcggaaatct attaccagga ggcttctgac
     1321 gagcagttag cgtatattcc tgccgcgcgg cggctcatga tcgaggacaa cgaggcgtta
     1381 atcgttattg atgctcagga gaacacgaaa tctctgaatg gcatcaatcc tgagcgtttt
     1441 gccatcgcgc gtaaagctgg cgaggaattg aatctgaaag tgttgcagcg taccaatgag
     1501 cgagacccct acgcgcctgg ctctctgcgc tggaacagga cgttgtttcc gaccgatgct
     1561 tacgcgcagg atgctgagat gtcgctggcc gagttcgaga attttgtcta tcacgcctgt
     1621 ttccttgatg atgatgaccc ggtcgcgcgc tggcaggagt tgagcgcgca gcaggagcgg
     1681 ctggtgcagt ggttgcatgg tcggcacacc gttcacctgc gcgggcagga taccgacctg
     1741 accctgtcca tcgaggggcg cacgttcatc aacgacgacg gtcactataa cctgcccggc
     1801 ggggagtttt ttaccggccc cgtcgagcag agcgcgaatg gcttcatccg ctatagtttc
     1861 ccctgctcgt ttcagggcag atcggtcgag gatgtgcgcc tgcgcttcga gaacggtgtc
     1921 gttgtagagg cgcgcgccgg gcagggacag gagtacctgg ataggatgct tgcgatggat
     1981 gagggggcgc ggcgcatcgg cgagtttgcc ttcggcaata accgcaacgt ggatcgctgc
     2041 acgaagcata cgctcttcga tgagaagatg ggcggaacgg tgcatcttgc tcttggcgca
     2101 agcatcccac agaccggcgg ggtcaatgtc tcggctctgc attgggatat ggtctgcgat
     2161 ctgcggcacg gcagcgagat tcgtgtcgat gacgaactat tcagcaaaga tgggcgattt
     2221 gtggtgtgaa gaagctgctg taaaaatgag aggggtgtga agacaa
//
LOCUS       DASZAK010000414         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_259533, whole genome shotgun sequence.
ACCESSION   DASZAK010000414 DASZAK010000000
VERSION     DASZAK010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259533"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VGT44_07240"
     CDS             complement(<1..1070)
                     /locus_tag="VGT44_07240"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV2580632.1"
                     /translation="MDLRNWFSKRQTRRRALRSIGMIAGAGLAVDAGVLAVERATAAA
                     LTSQPNPIQHILIACQENHTFDSYFGYYPNAGKYGVPANYSQPDGNGGTVTPQHPFLP
                     FVSDISHTWQAIHSELDNGKMDGFYTTDGSDAMDYYDSSDLQYYYGLAGAFTLCGNYF
                     CSVLGPTVPNRLSLVAATCGGYTLDSINSGQVDYPTIADLLDAHKITWKCYNLGLGLG
                     SNGSLEHFNGLTCFAKWQNDPRLQFSEDDYHNDLNAGTLPQVSFLITESTISEHPPFD
                     IQWGQQKMSDVIAKLMASSSWKSSALFLTYDEGGGFFDHVAPPQVDAYGLGFRVPTLV
                     ISPYAKRGYISGQVYEHSSMLK"
     gene            1240..>2572
                     /locus_tag="VGT44_07245"
     CDS             1240..>2572
                     /locus_tag="VGT44_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2580633.1"
                     /translation="MRYAILTDVHANLEALQAVLAKIDEIANEEPIDQMWFLGDLVGY
                     GPDPNECIRVLRERTDVMIAGNHDWAAVGKIDLEDFSAAARVSAEWTAEQLTPEHRDF
                     LINLPERLEIDGCTLVHGSPFGPLWEYLTSEALAERSFLYFDTRYCLVGHTHIPAIFI
                     QPDAANIPTLPLSDVEQAPPVTLVVPDANARANGANNHHEQTGDSDAHDEAHAAEPPA
                     VKDSAGDASEADDDDETVELVMAGQQSTLAADSLAEPAASAYQEPRGNASPSADSTDT
                     PGADTPDDDETETAFSLTQEDTPESQVKSDSPATDGDDDDDATAIAPLDYSAIRSFVG
                     AGSNESEEAEPDAGRSIDDESIKDDEGEDADDDEDDDDAEVRTPAGAIANAEERLTSE
                     IEELLSLLGLSQSMVEVTNQMLTPPEGPWELPPEHRAIINPGGVGQPRDGDP"
BASE COUNT          573 a          716 c          787 g          496 t
ORIGIN      
        1 aatttcagca tggaggagtg ctcgtagacc tgtcccgaga tgtagccgcg tttcgcatag
       61 ggcgagatga ccagcgtggg gacgcgaaat cccaggccat aggcgtccac ctgcggaggc
      121 gcgacgtgat cgaaaaagcc accgccttca tcgtaggtca ggaagagggc cgagcttttc
      181 caggaactcg acgccatcaa tttggcgatg acgtcggaca tcttctgctg tccccactgg
      241 atatcgaagg gaggatgctc gctgattgta ctctcggtga tgaggaacga gacctggggc
      301 agcgttccgg cgttcaggtc gttgtggtag tcgtcttcgc tgaattgcag gcgggggtcg
      361 ttctgccatt tggcgaagca ggtcaggccg ttgaagtgtt cgagcgagcc gttgctgccg
      421 aggccgaggc ccaggttgta gcacttccag gtgatcttgt gcgcgtcgag caggtcggcg
      481 atggtgggat agtctacctg gccggagttt atggaatcaa gggtgtagcc accgcaggta
      541 gcggcgacca gcgagaggcg attggggacg gtcggaccca gcacggaaca gaagtagttg
      601 ccgcacagcg tgaacgcgcc cgccagcccg tagtagtact ggagatcgga actgtcgtag
      661 taatccatcg cgtccgagcc atcggtggta tagaaaccat ccatcttgcc gttatccagt
      721 tcgctgtgga tcgcttgcca ggtatgcgaa atatcgctca cgaatggtag gaatgggtgc
      781 tggggagtga cggtgccgcc gttgccatcc ggctgggaat agttggcggg gacaccgtac
      841 tttccggcgt tgggataata cccgaaataa ctgtcgaatg tgtgattttc ctggcaggca
      901 atcaagatat gctggatagg attcggttgg ctcgtcagag cggcagccgt cgctcgttcg
      961 acggccagaa cacccgcgtc aaccgccagt cccgcgcccg ctatcatgcc gatgctgcgc
     1021 agcgcgcgcc gtcgcgtttg acgtttgctg aaccagtttc gcagatccat atcttccctt
     1081 tctctaaaaa aatatgcgcc cgtgcaaagt gaattgaggg agccattaga aagaacgcaa
     1141 ttgacaagca gattgccgta tcccctagtc agcccgttat aaacatggta gaatgtcctc
     1201 gaatattcgc cattgttatt tgaatgagtg tgctgaatta tgcgttatgc cattttgacc
     1261 gatgttcatg ccaacctgga agcgttacaa gcagttctgg caaagattga tgagatcgcg
     1321 aatgaagagc caatagacca gatgtggttt ctgggcgacc tggtgggcta tgggcctgac
     1381 ccgaatgaat gtattcgcgt gctgcgcgag cgcaccgatg tgatgatcgc gggcaatcac
     1441 gattgggccg ccgtcggcaa gatcgacctg gaggatttca gcgcggctgc gcgcgtctca
     1501 gccgagtgga ccgccgagca gctcacgccc gaacaccgcg attttctcat caacctgccc
     1561 gaacgcctgg agatcgacgg ctgtacgctg gtgcatggca gccccttcgg cccgctctgg
     1621 gaatatctga cttcggaggc gctggcggaa cgcagtttcc tctattttga cacgcgctac
     1681 tgtctcgttg ggcataccca catcccggcc atcttcatcc agcccgacgc cgccaacatc
     1741 ccgacgctcc cgctcagtga tgtcgaacag gccccccctg tcacgcttgt cgtgccggat
     1801 gcaaacgccc gggcgaacgg cgcgaataac caccacgaac agaccggcga tagcgatgcg
     1861 catgatgagg cgcatgcagc cgaaccgcca gccgtaaagg atagcgcagg tgacgccagc
     1921 gaagccgacg atgatgacga gacagttgag ctggtcatgg cgggacagca atccactctc
     1981 gcggctgact cccttgcaga accggcagca agcgcctatc aagagccgcg cggcaacgct
     2041 tccccgtcag cagacagcac agatactccc ggcgctgata cccctgatga tgatgaaacc
     2101 gagacggcct tttccctcac ccaggaagac acgcctgaga gccaggtcaa gagcgattcc
     2161 ccagccactg atggtgacga cgatgacgat gcaaccgcaa tagctccact tgattattcg
     2221 gctataagaa gttttgtggg tgcgggaagt aatgagagcg aggaagctga gcctgacgcc
     2281 ggtcgaagca tcgacgatga aagtatcaaa gacgacgagg gcgaagatgc cgacgacgat
     2341 gaagacgacg acgacgcgga agtacgcaca ccggcgggcg cgattgcgaa cgcggaagag
     2401 cgcctcacca gcgaaatcga agagctgcta agcctgttgg ggctgagtca gagcatggtc
     2461 gaggtcacaa accagatgct cactccgcca gagggacctt gggaattgcc gccggaacac
     2521 cgcgccatca tcaaccctgg cggcgtgggc cagccgcgcg acggcgaccc gc
//
LOCUS       DASZAK010000415         1844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_471886, whole genome shotgun sequence.
ACCESSION   DASZAK010000415 DASZAK010000000
VERSION     DASZAK010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471886"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            427..>1844
                     /gene="cysS"
                     /locus_tag="VGT44_07250"
     CDS             427..>1844
                     /gene="cysS"
                     /locus_tag="VGT44_07250"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV2580634.1"
                     /translation="MIEISDRPIYLYNTLTRSKELFRPLNPPRVGIYNCGPTVYKDIH
                     VGNFRTFMTTDWLRRMLEYNGYEVFLVRNITDVGHLHDVDDERSGEDRLEHEARTTGR
                     SAYEIAAYYTQQYLADAAELNILPAHINPRATDHIPDMLEMTVKLIEKGLAYTVDGNV
                     YYDVSRFPGYGQLSGNTVENLREGASGRIQLEIAEDKDAPEDFALWKRGDESRQMNWP
                     SPWGIGFPGWHIECSAMSTRYLGEQFDIHTGGMDLIFPHHEDEIAQSQGVSDKRPVQF
                     WVHNEFLIFGSIKMSRSKGNVVLLSTLKERGIDPLAYRYLLLTAHYRSKLNFTDESIT
                     AAQNGLNNLRADLAELPLPGADDLQSGWSEEAQRAHDAFHAAINDDLDLPAALSLTRE
                     VARNSKIAPAERRRLVLDFDRVLGLRLDTVEPKKPRVISDAARALVLARDEARAARDW
                     KRSDQLRDQLIALGFEVRDTPGG"
BASE COUNT          444 a          515 c          505 g          380 t
ORIGIN      
        1 tatggtatac tatagccaaa cgcactagct aagtctgagc taatttggag aagattgtga
       61 accacttgag ccatcataag cactattgca cctgtcctgg cgttcgcaga ggaaacatcg
      121 gtagctcagc cctctgcgaa cgtaacgacg tgccctcgat cccacagtaa caagcttgtc
      181 tttccgcacg tcccccgcgt ctgagctact acctttccga ccggctgacc gtaggtagaa
      241 cgcagtactt cctttggtct tgcctgacgg acgcagtccg tagacgcgca gccgtctggg
      301 gtggtgcgtg gccctcggag agtccaagac ccatttcaag gcacgttgcc ggtcggaata
      361 agatcaccta ctacctcgca atgaacctga ttgcactgga ctatacaaga gaggagatat
      421 acgctcatga tcgaaatttc ggacaggccg atttacctgt ataacacgct aaccagaagt
      481 aaagaactgt ttcgaccgct caatccgcca cgagtaggaa tatataactg cggaccaacc
      541 gtctacaaag atatccatgt aggcaacttc cgcaccttta tgacaacgga ttggctgcgc
      601 cgcatgctcg aatacaacgg ctatgaggtg ttcctggtgc gtaacattac cgacgtaggg
      661 catctacatg atgttgatga tgagcgcagc ggtgaggata gattggagca tgaggcacgc
      721 acgaccggcc gctcagcata cgaaattgcc gcctactata cgcagcagta cctggcagat
      781 gcggctgaac tgaacatcct ccctgcgcac atcaatccca gggcaaccga tcacattcca
      841 gatatgctgg agatgaccgt gaagctgatc gagaagggcc tggcatatac tgtagatggc
      901 aatgtctatt atgatgttag ccgcttcccc ggttatgggc agctctcagg taataccgtt
      961 gaaaacctgc gcgaaggcgc aagcggccgg atacagttgg agatcgcaga agacaaagat
     1021 gcacccgaag actttgccct atggaagcgc ggagacgaaa gccggcagat gaactggccc
     1081 agtccctggg gcatcggctt ccctggctgg cacatcgaat gctctgccat gtccaccagg
     1141 tacctgggag agcagttcga cattcacacc ggcggtatgg acctgatctt cccgcatcac
     1201 gaggatgaga tcgcccagag ccagggcgtg agtgataaac ggcccgtaca gttctgggta
     1261 cacaacgaat ttctgatctt tggtagtatc aagatgtcac gctccaaagg gaacgtcgtc
     1321 ctgctaagta cgcttaaaga gcgaggcatc gacccgctgg cctatcgcta cctgctgctg
     1381 acggcgcact accggtcgaa gctcaacttc accgatgagt caatcacggc agcgcagaac
     1441 ggcctgaaca acctgcgtgc tgaccttgca gagttgcctc tacctggcgc agacgactta
     1501 cagagtggct ggtcggaaga agcccagcgg gcgcacgacg cgttccacgc ggccatcaac
     1561 gatgatctgg atttgccggc tgcgctgagc ctcacccggg aggttgctcg taatagcaag
     1621 attgcgccag cggaaaggcg ccgcctggtc ctcgactttg atcgagtgct cggtctgcgc
     1681 ctcgataccg ttgagcctaa aaagccgcgc gttatcagcg atgcagcgcg agcattagtg
     1741 ctcgcgcgtg acgaagcccg cgcagctcgc gattggaaac gctccgatca gttgcgcgat
     1801 cagttgatcg ccctgggctt tgaagtacgt gatacacccg gtgg
//
LOCUS       DASZAK010000416         6403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_614732, whole genome shotgun sequence.
ACCESSION   DASZAK010000416 DASZAK010000000
VERSION     DASZAK010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614732"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VGT44_07255"
     CDS             complement(<1..215)
                     /locus_tag="VGT44_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580635.1"
                     /translation="MRKSDGLHKLALTALLITSTLVLVVVTAWGVRLLALRNQAAAQP
                     QPTVSGIAGFPMSGDPAPDFTLVDQFG"
     gene            complement(253..1224)
                     /locus_tag="VGT44_07260"
     CDS             complement(253..1224)
                     /locus_tag="VGT44_07260"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEV2580636.1"
                     /translation="MNIWVDANGWPVPPAVLLGCLAAEILYFRGWQVIVKGIRAKEAA
                     RARNPALFTGAPIGEYRWDKWLWHGIYFLGAIFALLLASSAPIDYFSGRLLWVHMIQH
                     LLLLVVVAPLMVAAAPLIPFWLGLPTWSRRLVKKLGAGLFFYRLGQGLRHPTLSCALF
                     IIGIWIWHWPALYDLALTNNAIHDWFEHTTFLAVSILFWSQVISSPPLYSRLGYLGRL
                     GCVGFAIAQNVVLAALIGFAQVPLYAPYAHLIQASGALTALQDQQLGAGIMWTFGDLP
                     FGIAFSVLLHRWLFSLSDDASADKVALAVNAHFSTQGEAPGNTKSAL"
     gene            complement(1247..1867)
                     /locus_tag="VGT44_07265"
     CDS             complement(1247..1867)
                     /locus_tag="VGT44_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580637.1"
                     /translation="MTTSSASLEHAHEHESHGLELHVRVARARACSLMFILSDVLSVF
                     AILAAGGYLSALNTEGQFRAAGDNPAAFLPGLLIAIALVLSGLGYYWWDRRLRQGGAT
                     GQQLFFVLSWVLMIAAMVGQTWISVTLNRAYTTPYDAYESVITLLSWFTAVHLLLTAF
                     LGLLLFGRGLRGRLVGFEYLVQAAGYWWYYTVIASLLMWLFSMLLS"
     gene            complement(1889..3784)
                     /locus_tag="VGT44_07270"
     CDS             complement(1889..3784)
                     /locus_tag="VGT44_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEV2580638.1"
                     /translation="MTVAIEVKRPNLVQSLFPSVVSGILLGVIGAAAAGLIVNAAMQG
                     SNLDATVCAVYVGWLLFFFIGIGAFNGVFGWGFARREPTIAEEMQLAGKDQGLWRYFR
                     YTTDHKVVGMQYLATVFVLFFVGSMGAFMIRLEQSTPGALFFNPSTYNTIVGMHGIIM
                     IASTIIMVSGPFGNFILPIMIGARDMAFPRLNALSYWLLFSAIPILLSTLFLGGFPTG
                     WTGYAPLSVQSLTPGMDAYCFLIIVFGISVTVAAMNILTTVLIMRTKGMTWGRLPIFV
                     WGVMLSVGLGLTAFPSFIISQTMVLMDRVFQTSFFVASYGGNNWLYEHLFWFMGHPEV
                     YVIALPAMAVAAEVTAVFTRKPVFGYRLLLSGLVGICVLSVIVWGHHLYLSGSENALD
                     GPYMLDTELISIPTGLFFLVLIGTLWRGRIWVTVPLLFVGGMLVNFVIGGITGIYLAD
                     LPTDEILHGGMFVTAHFHFTLVGSMVFGFMAGLYYWFPKMLGRRLDPFLGRLHFWLFE
                     IGFVGTFTALFYAGLQGEPRWSANVAPPFATANLIASLFAILIAASVFTLVYNVIITL
                     VRGERAVANEWGAKTLEWSVPTPVPLENFEELPVVTSLPYDFTSPAAAAEGGSVVEAP
                     LQPQPES"
     gene            complement(3781..4560)
                     /locus_tag="VGT44_07275"
     CDS             complement(3781..4560)
                     /locus_tag="VGT44_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEV2580639.1"
                     /translation="MSEPNIEGSPAGASPPRGEPNHLRRALIIWGILSVIGIALVILL
                     VPLILPASASNNTSTDDFTIVVLTVLAVPVSFFVFVFLGYSMFAFKVKDRPTEAAVPL
                     QPRPGLQIGWLGITSVLCLFLLIWGMFAFYQETSASSNNTLVVQVTAQQWLWTFYLPQ
                     YGVSNNAQQLELPVGRPVQFTVTSEDVLHGFSIDALGVRVDANPGEMTTTPVVTPTQI
                     GQYRVACVELCGLYHSYMWSAVSVVSNSTFNSWITSLGGNP"
     gene            complement(4571..5308)
                     /locus_tag="VGT44_07280"
     CDS             complement(4571..5308)
                     /locus_tag="VGT44_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580640.1"
                     /translation="MRQHSSGFYLGIAAAIILGFGVVAGIVIGLRSDQGLGYEPNVLQ
                     AIKTGPNSATLQLATYPDSHVCHADAGALQYNWVTYCSGGSDPTAQGTTLEVPPNSVI
                     TVIIKQYDSATSLINDYFRQVRGTIGGVALLNNKPYSEVDATAPGHTFTLQSTPDSPY
                     PIFVSVPLVGVKSGAAANVTINGSSYPTPNIISFQFKTGPAGITYIWHCYDPCGDSVF
                     ARKPPYGFSGPMSTTGFMAGTMTVASY"
     gene            5892..>6403
                     /locus_tag="VGT44_07285"
     CDS             5892..>6403
                     /locus_tag="VGT44_07285"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV2580641.1"
                     /translation="MSDATDEYLPRQESTLQKKHRRKILLFSGVSLLNVGLLALLLTQ
                     LLTPAPHAMTDPLIGHAAPGFTLPQSQSPTQKLSLSNFMGKPVVLIFWASWCDPCKQE
                     EPILESAWKQMQTQGKDVVFLGINFQDSTSNATSFLQQYGITYPNVVDANGSVANAYG
                     ITSLPDTFFI"
BASE COUNT         1576 a         1760 c         1805 g         1262 t
ORIGIN      
        1 tgaccgaact ggtcgacgag ggtgaagtcc ggggcgggat cgcccgacat gggaaagccg
       61 gcgatgccac tgacggtcgg ctgtggctga gcagccgcct ggttgcgtaa tgcaagaagc
      121 ctgactcccc aggcggtgac gacgaccagc acgagcgtcg aagtgataag caaggcagtg
      181 agagccagtt tatgtaaacc atccgatttt cgcatggtca aatcctttca cctggcagct
      241 gcgtatctgt cttcacagcg cgctctttgt atttcctggc gcttccccct gcgtactgaa
      301 atgcgcgttc actgcaagcg cgaccttatc cgcgctggca tcgtccgaga gcgagaaaag
      361 ccagcgatgc agaaggacgc tgaacgcgat cccgaaaggg agatcaccaa aagtccacat
      421 gatccctgct ccgagttgct gatcttgcag cgcggtgagg gcgcctgatg cctgaatcag
      481 gtgggcatag ggagcataca aagggacctg agcaaaaccg atcagtgctg ccagtacaac
      541 gttttgcgcg attgcaaatc ctacacatcc aagccgtccg agatagccca ggcgggagta
      601 cagcggaggt gaggagatca cctgtgacca gaacaggata ctgaccgcca ggaaggttgt
      661 atgttcaaac caatcgtgaa tggcgttatt tgtcagcgcg agatcgtaga gcgcaggcca
      721 gtgccatatc cagatgccga tgataaacag cgcgcatgag agggttggat gccgcagccc
      781 ctgtcccagg cggtagaaga aaagcccggc ccccagtttt ttcaccagcc tgcgagacca
      841 ggtcggtaat cctaaccaga aagggatcag cggggctgcg gccaccatca gcggcgccac
      901 aacgaccagc aacagcaggt gctggatcat atgaacccac aacagccgac cggagaagta
      961 atcgatgggg gccgacgaag ccagaaggag agcgaagatc gcgccgagaa agtagatgcc
     1021 gtgccagagc catttgtccc agcggtattc accgattggc gcgccggtga agagggccgg
     1081 gttcctggcc ctggcagctt ccttcgctcg tatgcctttg acgattacct gccacccacg
     1141 gaagtagaga atttcggcag caagacaccc aagcagcaca gcaggtggga caggccaccc
     1201 gtttgcatca acccagatgt tcaatactca cctccggctg actgtcttat gaaagcagca
     1261 tgctgaacag ccacatcagc aggctggcga tcaccgtata gtaccaccag tagccagcgg
     1321 cctggacgag gtattcaaac cctaccagcc tgccacgcag cccccgcccg aaaagcagta
     1381 gaccgaggaa tgctgtgagc agcaagtgta ccgccgtaaa ccaggaaagc agcgtaatca
     1441 cactttcgta ggcatcgtaa ggggtcgtat acgcccggtt cagggtgaca ctgatccagg
     1501 tctggcctac catggctgcg atcatgagta cccaggaaag cacgaagaat agttgctgcc
     1561 cagtcgctcc gccctgtcgc aatctgcgat cccaccagta gtagccgagt ccgctcagca
     1621 caagggcaat ggctatcaac agcccgggca ggaaagcggc gggattatcc ccggcagccc
     1681 tgaactggcc ttcagtattg agggcgctga gatagccacc cgccgccagg atagcgaaga
     1741 cggataacac gtccgacaga atgaacatga gcgagcaggc gcgggcgcgc gccacccgca
     1801 catgcagctc aagcccgtgc gattcatgtt catgcgcgtg ttctaaggac gcactcgatg
     1861 ttgtcataat ctctctcctc cggttctatc aactctctgg ttgtggctgt aaaggcgctt
     1921 ccacgacgct accgccctcg gctgccgctg cgggcgatgt aaaatcgtag ggcagtgagg
     1981 tcacgacggg cagttcttca aaattttcca gaggaaccgg cgtcgggacg ctccactcga
     2041 gcgtttttgc gccccactcg ttggcaacgg cgcgctcacc tcgcacaagc gtgataatca
     2101 cgttgtagac gagcgtaaag acggacgcgg cgatcaggat cgcgaacagg ctggcgatca
     2161 aattggcggt cgcaaacggg ggcgcaacat tggccgacca gcgcggctcg ccctggagac
     2221 ccgcgtagaa cagcgctgtg aatgttccga caaagccgat ttcaaacagc cagaaatgga
     2281 gcctgcccag gaagggatcg agtcgtcgcc ccaacatctt cggaaaccag tagtagagtc
     2341 cggccataaa tccgaagacc atgctgccga ccagggtgaa atggaagtgc gccgtgacaa
     2401 acatgccgcc atgcaaaatt tcgtcggtcg gcaggtcggc caggtagatg ccggtgatgc
     2461 ctccgataac gaagtttacc agcatccctc ccacaaacag cagcggaacc gtgacccaga
     2521 ttctgccacg ccagagcgtg ccgatcagca ccaggaagaa aaggccggtg ggtatagaga
     2581 tcaactccgt atcgagcatg taggggccat ccagcgcgtt ctctgagccg ctcaggtaga
     2641 ggtgatgtcc ccacacgatc acgctcagga cgcaaatccc gacgaggccg ctcaggagca
     2701 gccggtagcc aaagaccggc ttgcgcgtga agaccgccgt aacctcggca gctactgcca
     2761 tggccggcag cgcaatcacg tacacctcgg ggtggcccat gaaccagaac aggtgctcgt
     2821 acagccagtt attgccgcca taggaggcaa caaagaagga ggtctggaag acgcggtcca
     2881 tcaggaccat cgtctgcgag atgatgaagg aagggaaggc cgtcaaccca agcccgaccg
     2941 agagcattac gccccacacg aagatcggga gtcgtcccca ggtcatgcct ttcgtgcgca
     3001 tgatcagcac cgtcgtcagg atattcatgg ccgcaacggt cacggagatt ccaaagacga
     3061 tgatcaggaa gcagtatgca tccatgccgg gcgttaacga ctggacagaa agcggcgcgt
     3121 aaccggtcca gccggtcggg aatccgccga gaaacagggt ggaaaggaga atgggaatgg
     3181 cggagaacag cagccagtaa ctcagcgcgt tgaggcgcgg aaaggccatg tcgcgcgcac
     3241 cgatcatgat gggcaggata aagttgccaa aaggaccact gaccatgatg atggtagatg
     3301 caatcatgat gatgccgtgc atacccacaa tggtgttgta ggtgctgggg ttgaagaaca
     3361 gcgctccagg cgttgactgc tccagccgta tcatgaatgc gcccatcgag ccaacgaaaa
     3421 agagtacaaa cacggttgcc aggtattgca tcccaaccac tttgtggtcg gtggtatagc
     3481 ggaagtagcg ccagagtccc tgatcttttc ctgccagttg catctcttcg gcaatcgttg
     3541 gttcgcgacg cgcaaacccc caaccgaata caccgttgaa ggctccgata ccgataaaga
     3601 agaacagcag ccagcctaca tagacagcac atacagtcgc atccaggtta ctgccttgca
     3661 tcgcagcgtt cacaatcagt ccagctgccg cggcgccaat tacaccgagc aggatgccag
     3721 aaacgacact cggaaaaagg ctctggacga gatttgggcg tttgacttct atggctacag
     3781 tcatggattt cctcccaggc tagttatcca ggaattgaac gtcgagttac tgaccacgct
     3841 caccgccgac cacatatagg agtgataaag cccgcacaat tccacgcagg ccaccctata
     3901 ctgcccgatc tgcgtcggcg ttacgaccgg cgtggtggtc atctctcccg ggttagcatc
     3961 gacgcgcacc ccaagcgcat cgatggaaaa gccgtgcagc acatcttccg aggtgacggt
     4021 aaactgcacc gggcgaccca ccggcagttc aagctgctgg gcattgttgg acacgccata
     4081 ttggggcagg taaaatgtcc acaaccactg ctgcgcggtt acctggacaa ccagtgtgtt
     4141 attggacgag gcggaggtct cctggtagaa ggcaaacatg ccccagatga gaagaaacag
     4201 gcacagcacc gacgtgatgc ctaaccagcc gatctgtagc cccggcctgg gctgtagggg
     4261 aaccgcggcc tctgtgggcc tgtccttgac cttgaatgcg aacatgctgt agccaagaaa
     4321 gacaaaaaca aagaacgaaa ctggcacggc cagcactgtc agcacgacaa ttgtgaaatc
     4381 atcggtggat gtattattgc tggcagacgc cgggagtatc agcgggacca gcagaatgac
     4441 cagcgcaatc ccaatcacgg agagtattcc ccagataatg agcgcgcgac gcagatgatt
     4501 gggttcccct cgcggcggcg aggcgccggc agggcttccc tcgatgttag gttcagacat
     4561 cctgtccccc ttaataactt gcgactgtca tcgtgcctgc catgaaaccg gtggtcgaca
     4621 tgggcccgct aaaaccatag ggaggtttgc gagcaaacac actatctcca catgggtcat
     4681 agcagtgcca gatgtaggtg atgccagccg gtcccgtctt gaattggaaa gagatgatgt
     4741 tgggcgtcgg gtagctgctg ccattgatag tcacgttagc ggcagcgccg gatttgaccc
     4801 caacgagcgg gacgctgacg aatatcgggt agggagagtc aggtgtcgat tggagggtaa
     4861 aggtgtgacc tggggcggtc gcgtcgacct cgctataggg cttattattg agcagcgcga
     4921 cgccgccaat agttccccgc acctgtcgga agtaatcgtt gatcaagctt gttgcgctgt
     4981 cgtattgctt gatgataacc gtgatgacac tgtttggtgg aacttcgagt gttgtgcctt
     5041 gagcggttgg atcgctccca cctgagcaat acgtcaccca gttgtattgc agcgcccccg
     5101 cgtcggcgtg gcagacatga ctgtcgggat aggtggcgag ttgaagcgtg gcgctgttcg
     5161 gcccagtttt gattgcttgc agcacattgg gttcgtagcc aagtccttga tccgatcgca
     5221 gacctatcac gattccggca actacgccga agcccaatat aatcgctgct gcaatgccta
     5281 aataaaagcc tgatgaatgc tgtctcaatg cagtcctcct tagtctgtgg ttttggcgcg
     5341 aataaccagc taatgcggca aaccagttga tgctgaaaac cagaagaggc tcattctctt
     5401 ctgtcggcta acgagtgtgg ctctatgcat tgcacctgtt ggacatctcg gagttgggat
     5461 gatcgcaaaa tagatgtcca cggtttcagg aactcccccg atttcctgat ttattgttcg
     5521 attttcgtag ggaaacagca ggtatggaga agatcccttg cctgggttcc tgttctttcg
     5581 ggtggaatac cctcctttct cgaatcgaga agaattacaa tttataaatt atcctcgtct
     5641 cagtgtaaga tgctttgggg agtgaagcaa gtggactgtt cgtgtatatc gaacaatctt
     5701 aatttttctc attagggcag cctaacatga tcttctcctc gtcgctgtag agctggcagg
     5761 agcgctattc cggaacacaa tctggtataa ctggagctgc cacagggctt gaatatttac
     5821 gcgaatggat gttaacatgg agccagggta acgccgacaa aaatgaacca tccgtggagg
     5881 tagaccttca tatgtccgat gccaccgatg aatatctacc caggcaggag tcaacgctac
     5941 agaaaaagca caggcgcaaa attttgctat ttagcggtgt cagcctgctc aatgttggcc
     6001 tactcgcgct gctcctgacg caacttctga ctccagcgcc tcatgccatg actgacccgc
     6061 ttatcggcca tgccgcgcca gggtttaccc taccacaatc gcaatcccca acccaaaaac
     6121 tctcgctctc aaattttatg gggaagccgg ttgtcttaat tttctgggct tcgtggtgtg
     6181 atccatgtaa gcaggaggag ccgatactgg aaagcgcctg gaagcagatg caaacgcagg
     6241 gaaaagatgt ggtcttcctg ggcatcaact ttcaagactc aaccagtaat gctacgagtt
     6301 ttttacagca atacggcata acgtatccaa atgtagtaga cgccaatggg tcggttgcca
     6361 acgcttacgg cataacatcc ctgccggata ccttcttcat cga
//
LOCUS       DASZAK010000417         1601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_542553, whole genome shotgun sequence.
ACCESSION   DASZAK010000417 DASZAK010000000
VERSION     DASZAK010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542553"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="VGT44_07290"
     CDS             complement(<1..1357)
                     /locus_tag="VGT44_07290"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEV2580642.1"
                     /translation="MQQRASAFDLRGLMLVAATGVCLAGILLEAGMQAALPAWGSVPA
                     FVLLIGAGAACAGVAACWRNRRARLVALLAACALLGAWRYATVSPVANPAALRAFIGT
                     KSLEVMGNIADEPRLEGRSSLLVIDAQQISFNQGRSWQDAHGQLEVLILGNLLDDPYS
                     PRYGDTVEAQGALQAPSPNSSPEIFASMTFPRLSIQQSGGNPFIAALYQLRTMLALII
                     ERSLPQPMAALLIALVLSLRTPSLLPLVPIFNVTGTAHLIAPSGLKITILAGVIGQVE
                     QRLTRGRRRKQTPQSQFRPLLPAEQRRHDRRQLPIKGLVIASIVLYTFLSGGGPSAMR
                     AGIMGIVLVLAPRNGRFYNVYTALAFSALLESAFDPFVLWDAGFQLSFLGTLGIVLLT
                     PLWMRLFHALERFWLGGYIAETFAVTLAAEVATLPIFATTFHTISLISPLTNVLAVAP
                     LA"
     gene            complement(1396..>1601)
                     /locus_tag="VGT44_07295"
     CDS             complement(1396..>1601)
                     /locus_tag="VGT44_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV2580643.1"
                     /translation="TATASGQLININTASSTDMRTVLHISSTTAQNIINYRTQHGPYT
                     SIDQLLQVVSQQIFSKIKGQVTV"
BASE COUNT          309 a          471 c          540 g          281 t
ORIGIN      
        1 acgccagcgg cgcgacggcg agcacattcg tcaagggcga aatcaacgag atggtgtgga
       61 aggtggtcgc gaaaatgggc agcgtggcga cttcggcggc cagcgtgacg gcgaacgtct
      121 cggcgatata gccgcccagc cagaagcgtt caagcgcatg aaacaggcgc atccagagag
      181 gtgtgagcag gacgatgcct agcgttccga ggaacgaaag ctggaagccg gcatcccaca
      241 acacgaaggg atcgaacgcg ctctccagca gcgcgctgaa ggccagggcg gtgtagacgt
      301 tgtagaagcg cccattgcgc ggcgcaagca ccagcacgat gcccatgatg cccgcgcgca
      361 tggccgatgg accgccgccg ctcaggaagg tatagagcac gatgctggcg ataaccagac
      421 ctttgatggg cagttgccgc cggtcgtgcc tgcgctgctc ggcgggcagc agtggccgga
      481 actgcgattg cggcgtctgc ttgcgccgcc gaccccgtgt caggcgctgc tccacctggc
      541 cgatgacccc ggcaaggatg gtaattttca ggccggaggg cgcgatcaga tgtgcggtgc
      601 cggtgacgtt gaagatggga actagtggaa gcaatgaggg tgtccgcagg ctcagcacca
      661 gggcaatcag cagggcggcc atcggctgcg gcagcgagcg ttcgatgatg agcgcgagca
      721 tcgtgcgcaa ctggtagagc gcggcaatga agggattgcc gccggattgc tggatggaga
      781 ggcgcgggaa ggtcatgctg gcgaaaatct ccggagagct attcggggag ggcgcttgca
      841 gcgctccctg cgcctcgacc gtgtcgccgt agcgcgggct gtacgggtca tcgagcaggt
      901 tgccgaggat gagtacttcg agctggccgt gcgcgtcttg ccaggatctg ccctggttga
      961 agcttatttg ctgggcgtcg atcacgagca ggctgctgcg cccttccagg cgtggctcgt
     1021 ccgcgatgtt gcccattact tccagcgact ttgtgccgat gaaggcgcgc aacgctgccg
     1081 ggtttgccac cggcgagacg gtcgcgtagc gccacgcacc gagcagcgca caggcagcca
     1141 gcagcgcgac cagccgcgcc cgtcggttgc gccagcaagc ggcgacgccc gcgcatgccg
     1201 ctccagcacc gatcagcaac acaaacgcag ggactgaccc ccatgcaggc aacgctgcct
     1261 gcattcctgc ttcgaggaga ataccggcca ggcagacccc tgtcgccgcg accagcatca
     1321 gaccacgcag atcaaatgcg ctcgctcgtt gctgcatact tctcaacacc cctacatgct
     1381 tctcacttgc ctcgtctaca ccgtgacctg gcctttgatc ttcgagaaga tttgttggct
     1441 gacgacctgg agcaactggt caatcgaagt gtacgggccg tgctgggtgc gatagttgat
     1501 aatattctgc gcggtagtgc tgctgatgtg caggacagtg cgcatatcgg tgctgctggc
     1561 cgtgttgata ttgatgagtt gtccgcttgc cgtggccgtg c
//
LOCUS       DASZAK010000418         3936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_116296, whole genome shotgun sequence.
ACCESSION   DASZAK010000418 DASZAK010000000
VERSION     DASZAK010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116296"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VGT44_07300"
     CDS             <1..1587
                     /locus_tag="VGT44_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2580644.1"
                     /translation="PRRVRVYKEKADLLQNLERYEQAVAVLEQLTHLDRENAAEYYLQ
                     QGKLLERLQYNDRALEAYNNAIKVDSNGAWLETTYQRKGDLLLNLKRYEEAVDAYKQA
                     VLCNAKVAVYHENMGNALLELERYEEALVAYETCLHLRKGEDPPTIFDKGRALSGLER
                     YEEALEAYNEAIRLSAGHPNPRFYHEKGVVLETLAQQAFEQEKQIRTQREPRLIMATP
                     AIHASKLAAYALQGTIEAKDGPVLSSVISPSSRVLAMGGASGVIRLMDMLTGMNLSTL
                     NGHTLYVWCLTFLSNDRLLSGSEDHHIKVWNLPKERILHDLVGHQHAVRCIAGTPDKQ
                     TIVSGSNDFTIKFWDLQTGQELLSRRKHTQRVLSIAISPNGSFFASAGWDKEIYVWDL
                     LTRQILHTLTGHTGYIYRVAISPDGKLIASGSADSTVHIWDSNTGKLIHILIGHKGSV
                     HGVDFCPDEPVLVSSGADGAIFFWNPLTGQKLYKLTEESPASDIIWSVNFSPDGQFLV
                     SRHGNINHHGDDVVKIWRKK"
     gene            1881..2813
                     /locus_tag="VGT44_07305"
     CDS             1881..2813
                     /locus_tag="VGT44_07305"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2580645.1"
                     /translation="MEQTTSNTGTTPDNTSPLRMLSNQMADAVERIGTSLVLVNGREG
                     QPASGVVYAPDLVLTAAHVIQRDEGITIFTHDKRTLPAQLAGRDSNSDLAVLRVADLG
                     LPAATPSDATARVGQLVMATGFTNEEGLMASSGVVSALGGPLRTRGGVLLERYIRTDA
                     IPYPGFSGGALIDTRGDLLGILTTGLINGVSLAIPTASARSIADTLTTQGHVKRGFLG
                     ISSQLVQVPAAQRSDSSQEYGLLIVKVDEGSPAQQGGILLGDILVTLDGHDIKDSEDL
                     QLLLSGERVGKTVPINVIRGSAPHTLNITIGERQ"
     gene            2827..3780
                     /locus_tag="VGT44_07310"
     CDS             2827..3780
                     /locus_tag="VGT44_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2580646.1"
                     /translation="MTQTTPFSTSSSTPTSPALPDIFSQALVDIYDRVKPGITQVRTD
                     GRGGGTGFILSEDGRLLTNNHVVASDNARVEVQLSDGRTFPAAVLHRNPRLDLALLKI
                     QGDHLAPLTPLTLADSTQLRVGELVFAIGHPWGQRWLITKGIVSAIRTAKLADDLATR
                     YIQSDVGLAPGNSGGPLLNADGQVVGINAMIFGGDLSVSIPSSVAQTWLAELPGRRVT
                     LGIEIQTVELPSFIRSSLPTSQASGVMVVGIRPERQAKHTDLLLGDILLELAGTPIKN
                     NAALLNTLATSQESERLAIKLLRGGKITEMEVTTLLLERGS"
BASE COUNT          957 a         1173 c         1041 g          765 t
ORIGIN      
        1 cctcgcagag tgcgcgtcta taaagaaaag gcagatctcc tccagaacct tgagcggtac
       61 gagcaagctg ttgctgtact cgagcaactc acccacctgg accgggagaa tgccgccgaa
      121 tactatctcc agcaaggcaa gttgcttgaa cgtctacagt acaatgatag agcgcttgag
      181 gcatacaata atgccattaa agtcgactcg aacggagcat ggttagaaac gacgtatcag
      241 aggaagggcg atctgttgct caacctcaaa cgctatgaag aagcggttga cgcctacaaa
      301 caggctgttc tgtgcaatgc aaaagtggca gtgtaccacg aaaacatggg caatgcgctg
      361 ctcgaattgg agcgatacga agaagccctg gtcgcctacg aaacctgcct ccatctcaga
      421 aaaggcgaag accctcccac tattttcgac aaaggccgcg ccttatctgg cctcgaacgc
      481 tacgaagagg cgcttgaagc ctataacgag gcgatacgct tgagcgcggg tcatccgaat
      541 ccccgcttct atcatgaaaa gggagtagtc ttagaaacgc tggcgcagca agcgtttgag
      601 caggaaaagc agattcgcac tcaacgagaa cctcgattaa taatggccac gccggcaatt
      661 catgcaagta agcttgctgc ctatgctttg caaggcacaa tcgaagcgaa agacggtccc
      721 gttctctcaa gtgtgattag ccccagcagt cgtgtactgg ctatgggagg agccagcgga
      781 gtaatcaggc tcatggatat gcttaccggc atgaatctga gtacgctcaa tggacacaca
      841 ttgtacgtct ggtgcctgac ttttctctcg aacgaccgcc tgctcagtgg gagtgaagac
      901 caccacatca aagtatggaa tctgcccaaa gaacgcatct tgcatgatct ggttggacac
      961 cagcatgccg ttcgatgtat cgccggaact ccagataaac agactatcgt aagcggcagc
     1021 aacgacttta ccatcaaatt ctgggattta cagaccggac aagagcttct ttcccgcaga
     1081 aaacacacac aacgagtatt gagcattgcc atcagcccga acgggagctt ttttgcaagc
     1141 gcgggctggg ataaagagat ctatgtctgg gacctgctta caagacaaat cctccatacc
     1201 cttaccggac atacagggta catctaccgg gtggcgatca gccccgatgg gaagcttatt
     1261 gcgagcggaa gtgcagattc aaccgtgcat atatgggaca gtaacacggg taaactcatc
     1321 cacattctga ttgggcataa aggttctgtt cacggagtcg atttctgtcc agatgagcca
     1381 gtacttgtaa gctcaggtgc ggatggcgca atcttcttct ggaatcctct aaccggccag
     1441 aaattgtata aactgacaga agaatctcct gcctctgaca tcatctggag tgtgaatttt
     1501 agccccgacg ggcagttcct cgtgagcagg cacggcaaca tcaatcatca tggtgacgat
     1561 gtcgtgaaaa tctggcgaaa aaagtaggag catgattata atttttcacg atcaagtccg
     1621 gtcatgtcat cctgagcgca gcgaaggatc tggcccgctg gatggatcgc ggcatgaccc
     1681 gcagatcctt cgctgcgctc aggatgacag gcttgcacgc tttcaaagtc cttaccgatc
     1741 ttggttgtga aatctcataa tcgcgtcctg gtaatttttc caggggaaaa actgttcaaa
     1801 tcaccgatgc agcatacttg cctgtggggt atagttatgg caggttccac aaaccatcat
     1861 ctagccagag agacccgaca atggaacaaa caaccagcaa cacaggtaca acccctgaca
     1921 atacttcacc attacgtatg ctttcaaatc agatggctga cgccgttgag cgcattggca
     1981 catcgcttgt cctcgtcaac gggcgcgaag ggcagcccgc gagcggcgtg gtctacgccc
     2041 cagacctggt gctgaccgcc gcccacgtca tccagcgcga tgagggcatc accatcttca
     2101 cccacgataa acgcacgctg cccgcgcagt tggccgggcg cgacagtaac agcgacctgg
     2161 cggtactgcg cgtggccgac ctgggcctgc cagccgcgac gccctcggac gcgacggcgc
     2221 gtgtcggaca attggtcatg gcgaccggct tcaccaacga agaaggactg atggccagtt
     2281 caggcgtggt cagcgcgctc ggcgggccgc tgcgcacacg cggcggcgtc ctgctcgaac
     2341 gctacattcg caccgacgcc atcccctatc ccggcttctc cggtggcgcg ctgatcgata
     2401 cgcggggaga cctgctcggc atcctgacga ccggcctcat caacggcgtc tcgctggcca
     2461 tcccgaccgc cagcgccagg agcatcgccg atacgctcac aacgcaggga cacgtcaagc
     2521 gcggcttcct gggcatcagc agccagctcg tgcaggttcc cgccgcgcag cgcagcgata
     2581 gctcccagga atacggcctg ctgatcgtca aagtcgatga aggcagcccg gcgcagcagg
     2641 gcggcatcct gctcggcgac atcctggtca cgctcgacgg tcacgacatc aaggactcgg
     2701 aagacctgca actgcttctc tccggcgagc gcgtggggaa aaccgtgccg atcaacgtca
     2761 ttcgcggcag cgccccgcat acgctcaata taaccatcgg cgaacgtcaa tagccaggaa
     2821 tcaaccatga cacagacaac acccttttca acatcttcat cgacacccac gtcgccagcg
     2881 cttcccgaca tcttctcgca ggcgctggtc gatatctatg accgggtgaa gccgggcatc
     2941 acgcaggtac gcaccgacgg gcgcggcggc ggcaccggct tcatcctgag cgaagatggc
     3001 cgcctgttga ccaacaatca cgtcgtcgcc agcgataacg ccagggtcga agtccaactt
     3061 tcggatggcc gcaccttccc ggctgccgtg ctgcaccgca atccgcgcct cgacctggcc
     3121 ctgctaaaaa ttcagggcga tcacctcgcg ccgctcacac cgctcacgtt ggccgactcg
     3181 acgcagctgc gtgtaggcga actggtcttc gccatcggcc atccctgggg acagcgctgg
     3241 ctcatcacga agggcatcgt cagcgccatt cgcacggcga agctggccga cgacctggcg
     3301 acacgctaca tccagtccga cgtgggcctc gcgcccggta actccggcgg cccgttgctc
     3361 aacgccgatg gacaggtcgt tggcatcaac gccatgatct tcggcggcga cctctccgtc
     3421 tcaatcccca gcagcgtcgc gcagacctgg ctagccgaac tgccaggccg ccgcgtcacg
     3481 ctcggcatcg agattcagac ggtagagctg ccatccttca tacgttccag ccttccaacg
     3541 agccaggcat ccggcgtcat ggtcgtcggc atccgccccg aacgccaggc gaaacacacc
     3601 gacctgctgc tcggcgacat cttgctcgaa ctcgccggta cacccatcaa aaataacgcc
     3661 gccctgctca acacacttgc caccagccag gaaagcgagc gattggccat aaaattgcta
     3721 cgcggcggca aaatcacaga gatggaggta acaactctgc tgctagaacg cggctcttaa
     3781 aactctacaa tcgttacaaa atgagataaa gtacctcgaa tcatggttgg caatctgaga
     3841 aaaatctacc tgcttgcatg gttcgagata ctttgtctgg cgaaaagttg tatattgatg
     3901 atatgtatgg tattctttgg gaaacgcact gcaaaa
//
LOCUS       DASZAK010000419         1856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_366090, whole genome shotgun sequence.
ACCESSION   DASZAK010000419 DASZAK010000000
VERSION     DASZAK010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366090"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..>1856)
                     /locus_tag="VGT44_07315"
     CDS             complement(75..>1856)
                     /locus_tag="VGT44_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEV2580647.1"
                     /translation="VLQSRPVTTRPPASVAQTTAPIPPGDDAWDREGEQPARPYDLWT
                     RTNFGENLPYPVTPLTSTGFPYVMGQGGQNEQEEAQVARRFYGRLYINEGAVMHDLSE
                     KYGLPSSIVDSMWGSRRRGKHKTKGKFRPWRLIRSSPGLLRLLMRLSKKRGPRQTPEQ
                     FFAQIDSWVSEFMRCDMTTLDDHALWAEFIPTWREHGAHVFRKNIAISAPAALNYGLL
                     DRVVGKWMKRKDITHDLVTVIAGIYSAEVGPLLWKMAQTLQEAGLADVVLDHPAPDAL
                     ALLRQEPAAQAFLAQFAEFLHRHGHRCPNEIEFLHPRWKEAPEQVVELLANYLRASDS
                     VDPIEAEKRQAWRREEAIATVEKRLGPVRRRIFHALLSRAQKAVRIRDNSRYYVTKFF
                     FPMRLLFAQLGQRWAERGWITTPEDVFFLTIAEVEKMATGADPAAKGANLPALIADRR
                     LAYEYWFTVVPPEVIGSNGQAVLDEEEQGTTLEGIAVSGGRASGIARIVLDPRDAARL
                     RAGEILVTQATDPGWTPVFPLVSGLVLEIGGQLSHGAIVAREYGIPAVVNVQGAMRRI
                     QDGQLVTIDGTQGKVYLHEPSTVISAA"
BASE COUNT          364 a          573 c          524 g          395 t
ORIGIN      
        1 ctggatgtat taaccaggcg tgagcttatt aacaaagtat atgtcgtgga acgcaacctc
       61 gtatctctgt acaattaggc ggcagaaatt actgtagaag gctcgtgaag atataccttc
      121 ccctgcgtcc catctatcgt aaccaactgt ccgtcctgaa tgcgacgcat ggcaccctgc
      181 acgttgacga cggcgggaat gccgtattcc ctggcgacaa tagcgccgtg cgaaagctgg
      241 ccgccgatct ccagcaccag gccgctcacc aatggaaaaa cgggcgtcca accggggtcg
      301 gtggcctgcg tgacgaggat ctcaccggcg cgcaggcgag cggcatcgcg cggatcaagc
      361 acgatgcggg cgatgccgct cgcgcgacca ccgctcacgg caatgccttc gagcgtcgtt
      421 ccctgctcct cctcgtcgag gacagcctgg ccattggagc ctatcacctc cggtgggaca
      481 accgtaaacc agtattcgta tgccagtcgc cggtccgcga tcaaagctgg cagattggct
      541 ccttttgcgg ccgggtctgc gcctgttgcc attttttcta cttcggcaat cgtcaaaaag
      601 aacacatctt ccggcgtggt gatccagcca cgttcagccc agcgctgtcc tagctgggcg
      661 aagagcagac gcatcgggaa gaagaacttc gtcacatagt atctgctatt atcgcggatg
      721 cgtaccgctt tctgcgctct ggacagcagg gcgtggaaga ttctgcggcg aacagggcca
      781 agccgcttct ccactgttgc gatagcctct tcgcgccgcc acgcctgccg cttctcggct
      841 tcgatgggat cgacgctgtc actagcgcgc aggtagtttg ccagtagttc gacaacctgt
      901 tccggcgctt ccttccagcg tgggtgcagg aactctatct cgtttggaca acgatggccg
      961 tgacgatgca ggaattccgc gaattgcgcg agaaacgcct gcgctgccgg ttcttgccgc
     1021 aacaaggcca gcgcatcggg cgcaggatga tcgagtacta catcagcaag ccccgcctct
     1081 tgcagggtct gtgccatctt ccagagcagc ggcccgacct ccgcgctgta gatgcccgcg
     1141 atgacggtca cgaggtcgtg cgtgatgtcc ttgcgcttca tccacttgcc aaccacacga
     1201 tctaacaggc catagttgag ggcggcgggc gcgctgatgg cgatattctt gcgaaagaca
     1261 tgcgctccat gctcgcgcca ggtggggata aactcagccc acagcgcgtg gtcatccagg
     1321 gtcgtcatat cgcatcgcat gaactcgctc acccagctat cgatctgcgc gaaaaattgt
     1381 tcgggcgtct gtcttggacc ccgcttcttg ctcaatcgca tcagcaagcg cagcagtcca
     1441 ggactgctgc gtatcagccg ccagggacgg aatttgccct tcgtcttgtg ctttccgcga
     1501 cggcgactgc cccacatcga gtcgactatg gacgacggca agccatattt ctctgacagg
     1561 tcgtgcataa cagcgccttc gttgatgtac aggcgaccat aaaaacgccg ggctacctgc
     1621 gcctcctcct gttcattttg tccaccctgc cccatgacat aagggaagcc cgtcgatgtg
     1681 agcggcgtaa caggatacgg cagattttct ccgaaattgg tgcgcgtcca caggtcatac
     1741 ggccgggccg gttgctcacc ctcacgatcc caggcgtcgt ctcccggtgg aataggtgcc
     1801 gtcgtttgcg ctacgctcgc cggtggtcga gttgtcactg gtcgtgactg caacac
//
LOCUS       DASZAK010000420         2744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_329732, whole genome shotgun sequence.
ACCESSION   DASZAK010000420 DASZAK010000000
VERSION     DASZAK010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329732"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VGT44_07320"
     CDS             <1..483
                     /locus_tag="VGT44_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580648.1"
                     /translation="VEIDARRRQSQQNSQIEHDLIGHPHLDFVVPPALRFLDPLLLQV
                     PRSRLLSALFDRALSLYTARGTMRLLRKSGYVDSFRHFRPRALGFTCPARAPAGRIDY
                     IMASPQLAIRLSDCYVLSEGNGVSGYQASDHLPVVAEFGEPIATRLAAPTPVMAGETY
                     "
     gene            complement(477..1616)
                     /gene="dnaJ"
                     /locus_tag="VGT44_07325"
     CDS             complement(477..1616)
                     /gene="dnaJ"
                     /locus_tag="VGT44_07325"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV2580649.1"
                     /translation="MASTKRDFYEVLGVARNASDDELKKAYRRLAKQYHPDANKEQGS
                     ESRFVEINEAYETLSDPQKRAVYDRYGHAGLNGGAGGMGFGDLGGFSSINDLFETFFA
                     GAAGAQRRSGTQRGADLRYELTITFEEAVFGCQKEIELPRWESCASCRGSGAQPGTST
                     TRCSACQGTGEIRRVQQSIFGQFVNVTMCERCRGEGRVITTPCEKCRGQGRVRNNRRV
                     VVNIPAGVDDGINVRVTGEGEVSSRGGTPGNLYVILTVKPHPFFKRENNDIQYELPIS
                     FAQAALGDEVEVPLVDSKTTMLKIPPGTQSGRSFRLKGMGVPVVHSSARGDQHVIVKV
                     VTPTNMSPEQKHLLEEFARLEKEQSELNDKNIFRQMFEKTKDVFQ"
     gene            complement(1661..>2744)
                     /locus_tag="VGT44_07330"
     CDS             complement(1661..>2744)
                     /locus_tag="VGT44_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV2580650.1"
                     /translation="NLPFITADASGPKHLNMTLTRSKLEQLVEDLIEKSRPPVMKALS
                     DAGIRPGEIQEVVLVGGQTRMPAVQTLVRRIFDRDPHRGVNPDEVVAVGAAIQAGVLM
                     GDVKDVLLLDVTPLSLGIETLGGVTTRLIERNTTIPTQKGQVFSTASDNQPSVEINVL
                     QGEREFAKDNRSLGTFILDGILPAPRGIPQIEVTFDIDANGIVNVSAHDKGTGRQQKI
                     TIVPSSGLSKDQIEKMVRDAETHAEEDKRRKEEVEERNQADSAAYRAEKSMNELGDKI
                     TSEQKGELESKIAEVRAALATDDVSRIKAAREALDQAFYKVSEGIYRQAGANGAAGGP
                     GSGADQEPSNQGGPQDDTIEGEYTES"
BASE COUNT          467 a          915 c          786 g          576 t
ORIGIN      
        1 gtcgagatcg acgcgcgccg ccggcaaagc cagcagaact cacagattga gcatgacctg
       61 ataggccatc cgcatctcga ctttgtcgta ccgcccgcgc tgcgcttcct cgacccactg
      121 ctgctacaag taccgcgcag ccgcctgctg agcgcgttat tcgatcgtgc gctttcgctc
      181 tacaccgcac gaggcacaat gcggctgctg cgcaagagcg gctacgtcga tagctttcgc
      241 catttcaggc cgcgcgccct gggcttcacc tgcccggccc gtgcgcccgc tggacgaatc
      301 gactacatca tggccagccc gcagctcgcc attcgcctct cagattgcta cgtactgagc
      361 gagggcaacg gcgtctccgg ctaccaggcc agcgaccacc tgccagtcgt cgccgaattt
      421 ggggaaccca tcgcgacgcg cctggccgca cccacgccgg tgatggcggg tgagacctac
      481 tgaaacacgt ccttcgtctt ctcgaacatc tgtcggaaga tgttcttatc gtttagctcg
      541 ctctgctctt tttccagtcg agcgaactct tcgagcaggt gtttctgctc agggctcata
      601 ttagtaggtg taacgacctt gacgatgaca tgctggtcgc cgcgcgcgct gctatgcacg
      661 accgggacgc ccattccttt gaggcggaaa gaacgcccgc tctgcgtacc gggcgggatt
      721 ttcagcatgg tggtcttgct atccaccagc ggcacctcaa cctcgtcgcc tagcgccgcc
      781 tgcgcgaagc tgatcggcag ttcgtactgg atatcgttgt tctcgcgctt gaagaagggg
      841 tgcggcttga ccgtcagaat gacgtagagg ttccccggtg tgccgccgcg cgagctgact
      901 tcaccctcgc cggtcacgcg cacattgatg ccgtcatcta ccccggcggg gatgttgaca
      961 accacgcgcc ggttgttgcg cacgcggccc tggccgcggc acttctcgca gggcgtcgta
     1021 atcacccggc cttcgccgcg acagcgctcg cacatcgtca cgttgacgaa ctggccgaag
     1081 atcgactgct gcaccctcct gatctccccc gtaccctggc aggccgagca gcgcgtcgtg
     1141 gacgtgccag gctgcgcgcc cgacccgcga caactcgcgc agctctccca gcgcggcagt
     1201 tcgatctcct tctggcagcc gaacacggct tcctcgaagg taatggtcag ctcatagcgc
     1261 aggtccgcgc ctcgttgcgt accagaacgc cgctgcgccc cggcagcgcc cgcgaagaag
     1321 gtctcgaaga gatcgttgat cgaactgaac ccgccgagat cgccaaaacc catgccaccc
     1381 gcgccgccat tcagcccggc atggccataa cggtcataca cggcgcgctt ctgcgggtcg
     1441 ctgagcgttt catacgcctc gttgatctcg acaaagcggg attcagaccc ctgctcctta
     1501 ttcgcgtccg ggtgatactg cttcgccagg cgacgataag cctttttcag ctcatcgtcg
     1561 ctggcattgc gagccacacc cagcacttca taaaaatcgc gtttcgtact cgccatgcgc
     1621 tggtcctcta tgtcagaaac aaaccggaaa acccggaagg ttacgactct gtgtattcgc
     1681 cttcaatggt atcatcctgg gggccgccct ggttggacgg ctcctggtcc gcgccagatc
     1741 ccggcccgcc agcagcgcca ttcgcgcctg cctggcgata gatgccctcg ctgactttgt
     1801 agaaagcctg gtcgagcgcc tcgcgagccg ccttgatgcg tgagacatcg tcggtagcga
     1861 gcgcggcacg cacctcggcg atcttcgatt ccagttcgcc cttctgctcg gacgtgatct
     1921 tatcgcccag ctcgttcatg ctcttctcgg cgcgataggc tgcgctgtcg gcctggttgc
     1981 gctcctcaac ctcttccttg cggcgcttgt cctcttcggc atgcgtctct gcgtcgcgca
     2041 ccatcttttc aatctggtcc ttcgagaggc cactcgatgg cacgatggtt atcttctgct
     2101 ggcgccccgt gcctttgtcg tgcgccgaaa cattgacgat accattggca tcgatgtcga
     2161 atgtgacctc gatctgcgga atgccgcgcg gagccggtag gatgccgtcc aggatgaagg
     2221 tgccgagcga gcgattatcc ttcgcgaatt cacgctcccc ctgcaagaca ttgatttcaa
     2281 cgcttggctg gttgtcgctg gcggtcgaga aaacctggcc cttctgcgta gggatggtgg
     2341 tgttgcgctc gatcagtcgc gttgtgacgc cgcccagcgt ctcgatgccc agcgacaatg
     2401 gcgtgacatc gagcagcagc acatccttca catcgcccat cagcacgccc gcctggattg
     2461 ccgcgccgac ggccactact tcgtccgggt tcacgccgcg atgcgggtcg cggtcgaaga
     2521 tgcggcgcac cagcgtctgt acagcaggca tacgggtctg gccgccgacc agcacgactt
     2581 cctgaatttc gccggggcga attccggcat cggagagcgc cttcatgacg ggtgggcgcg
     2641 acttttcgat caaatcctca accagttgct ccagcttcga acgcgtcagc gtcatgttga
     2701 ggtgtttggg gccgctggcg tccgccgtga tgaaaggcag attg
//
LOCUS       DASZAK010000421        22125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_10474, whole genome shotgun sequence.
ACCESSION   DASZAK010000421 DASZAK010000000
VERSION     DASZAK010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 22125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22125
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10474"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGT44_07335"
     CDS             <1..259
                     /locus_tag="VGT44_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580651.1"
                     /translation="QGLKANPDSLDLIVMLITLYLQQEDIAGLERAIKQLARLDPHYP
                     ALPLLRSMLPTLKATFRSGASKTGMPPLVLAPMPERPKKRR"
     gene            complement(256..1254)
                     /locus_tag="VGT44_07340"
     CDS             complement(256..1254)
                     /locus_tag="VGT44_07340"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEV2580652.1"
                     /translation="MPELSDAKRALLEKYLRGELSTQADTRDTAPPEAQAESTGPRVV
                     PIQARGSKRPFFFLHGQWQSMAFYCFPLAQGLGSDQPFYVVEPFTFEQQPVPPSFQEI
                     AAIHIKAIRAVQPEGPYLLSGWCNGALMAYEIARQLHEAGQQVDLLVLMNSMALVYPR
                     RYHMTRHALVLLGKLLRSGEEKQIDWYVRIRRARLYFLHVKDYFRIRIRKLQAALHLG
                     TAEQIERWGQRSVTFPRPSEWLPKAGTLRQDYESVFTWLTLGYKPPRLYPGKITFFWS
                     SADWSAKEPFRNGWLHVEKTQNVEIHVIPGKHMGLVTDQLPTLVETLRACLDTTRD"
     gene            complement(1274..2518)
                     /locus_tag="VGT44_07345"
     CDS             complement(1274..2518)
                     /locus_tag="VGT44_07345"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV2580653.1"
                     /translation="MTLSTQSSQTPGKKEPPLSLYHLLDPEVLANPYPLYQRLRTEDP
                     VHWDPFLHAWVVTRYADVVSVFQRFSANRTPTPEQLTSLGLEALTPLARVMVRQMLFL
                     DPPAHGRVRGLASKAFTPRRVEVLRSHIQDITNSLLDAVQQQGHMNVLADLAIPLPAI
                     VTAEMLGVPTTDWQQLTKWSADFAGVLGNFQHNPEHAAQVIQSLEGMVDYFRAAIHEQ
                     QAHPRDGLIHALVTAEIDGDRLTEDEVIANTIVTMVGGQETTTNLIGNGILTLLRHPE
                     EFEQLKTDLSLIPSAVEELLRYESPSQQTARLAPDDTELGGKAIRKRQAVIAVMGAAN
                     RDPERFPDPDRLDFQRQDNRHVAFAWASHFCFGAPLARIEGQIAFKTVLERMPDLRLD
                     PGPITWRENLGLRGLTALHVSF"
     gene            complement(2552..4441)
                     /locus_tag="VGT44_07350"
     CDS             complement(2552..4441)
                     /locus_tag="VGT44_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEV2580654.1"
                     /translation="MDTNNTEQEAQQLAAWNEATRLDYSTDLCVPALVARQAAAAPDA
                     VALVVGSQVLTYLELNQRANRIAHYLQSHGVRPNVLVGLCVERSVDMVIGLLGILKAG
                     GAYVPMDPAYPSDRLIYMLEDSQAAVLLTHHYLAERLPIEQDRMFCLDTDADMLARYD
                     SSNPRSDVTADDLIYVIYTSGSTGKPKGSLVTHNGLLNLVYWHQHTFGVTAADHATQV
                     ASPAFDATGWELWPYLTIGASVYLPDEDTRITPATLRDWLVRNGITISFLPTALAESV
                     MALDWPATTALRYLLTGADKLERYPSPNLPFAFINNYGLTEASVVNTSGRILPESRAD
                     RPPSIGRPIANTQLYILDEQMRQVPIGETGELYIGGHGLGKGYLNRPDLTAERFVANP
                     FSSEADARLYKTGDLAYFLPDGQVAFLGRADHQIKIRGYRIEPSEIVAVLNSHPAIQT
                     SLVVAREDTPGNKRLVAYIVPNAGAQVSLGSLRDTLTAQLPEYMVPTAFVPLEALPVT
                     PNGKIDRAALPAPDSGNIVRDGEATAPETPLEERLAAIVATLLNLETIGRDDNFFMLG
                     GHSLLGTQIIARVSGAFGVDLSLRTLFEAPTIRQLSEEIERLILIKLEMMSDQDVQRL
                     LEQVQ"
     gene            complement(4644..5234)
                     /locus_tag="VGT44_07355"
     CDS             complement(4644..5234)
                     /locus_tag="VGT44_07355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEV2580655.1"
                     /translation="MTEPAFYVHPTAEVSPDAQVGAGTRIWGQAHVREHAHIGRSCNI
                     GKGVYIDAHAQIGSNVKIQNHVSIFEGVTLEDGVFIGPHVCFTNDLLPRAITPDGRFK
                     SAADWEITPTLVKYGASIGAGAVILCGITIGEFALVGAGSMVTRDVAAHALVFGNPAR
                     QHGYVCRCARRLAEVQEQEDRLRGRCEVCGDIEFSR"
     gene            complement(5238..6290)
                     /locus_tag="VGT44_07360"
     CDS             complement(5238..6290)
                     /locus_tag="VGT44_07360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV2580656.1"
                     /translation="MRKVILLCIAACLYYSGLVRLARWWHRQCGPRLVILNYHRVCDG
                     DLRRHLLYLRHHYRILHLEAALVELSSLVKGKMNATQQKTLLALTFDDGYPDNYTHGF
                     ALASELQVPITIFLIPGYVQSGQRFWWRESKWLVRHARVDEVTLAGRVYHLGQAREQR
                     ELARAIDARLRFAASVQEREAFLTTIRQTLSITEGDTEVDEWERSLTWEEARTMDKSA
                     WVSFGAHTMHHPILAALADPREVLSELEESRAALEQELEHPIRAMAYPIGQMQYLGAD
                     TTRVVQQAGYDWAVTTIYGINTTETDPYLLKRIEVASIQHWLLMAVETAGLWGLFARL
                     RKVPFVQQHFTTTSWK"
     gene            complement(6283..7608)
                     /locus_tag="VGT44_07365"
     CDS             complement(6283..7608)
                     /locus_tag="VGT44_07365"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV2580657.1"
                     /translation="MKETVPLPFDENAQLPVAGADERAGLATALVKPAAAYDALVLDA
                     ILRQSLVSVRSLGSRGLRVASLEAGNKLNSVGTTPTFSSRWNRQKYIAPSYVQSTEPY
                     LRFLEEVLDQTRARVLITSSDGTLALVHQHREQLEKRVAIALAKDPALAIAINKDQTL
                     AIAAQLGIAIPRGVKVDSAQDVAAAVKEIGLPAVVKPVESWLWGELQSARLTCKLVTS
                     ADEARQAVEELTRLGGSTLFQQFLTGRREAVSLLYARGEFYARFAQWASRTNPQLGGT
                     SVLRQSIAIPPDIGEQSERLVRAIDLEGYSEIEYRRDNHGTPYLMEINPRLSASIEVA
                     VRAGVDFPYLLYQWANGDRIDVVKGYREGGWMRYLEGDFLTTAEAVLQRGRPGVAPPT
                     QAILAFFLSFFKPMSYDYLDWRDPLPVWTATTAFMNLLVRHLRRKGSHA"
     gene            complement(7629..8666)
                     /locus_tag="VGT44_07370"
     CDS             complement(7629..8666)
                     /locus_tag="VGT44_07370"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV2580658.1"
                     /translation="MRERVRLWIAACFYYSGLVKLAYWRLQRGGPRLVILNYHQASAS
                     NLRDHLLYLRRHYRLLHLEAALEELYGAQPPNKRLNDRRMPLVLTFDDGYRDNYTHAF
                     PLAQALQVPMTIFLVPGYIRSGERFWWLEGKRLAASVTTDEATIDGRIYRLSQPGERD
                     ALAHAIDTRLRSATSVEQREALLREVRQALRIRDEVIPGEEGERPMTWTEAREMDASA
                     WVSFGAHTMHHPVLAALADPLEIRREVEECRRVLEQELGHQVRAFAYPIGKPEHFGER
                     GVQAVKSAGYTWAVTTCEEVNTPQCDQYLLRRLPGVVEKHWLVMASELVGLLGIVSRV
                     NNYLNLRQVAK"
     gene            complement(8728..9948)
                     /locus_tag="VGT44_07375"
     CDS             complement(8728..9948)
                     /locus_tag="VGT44_07375"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV2580659.1"
                     /translation="MARKKPRPPLSTDLRDVVVVGAGPYGLSTTAHLRERGLNVAIFG
                     KPLKLWREHMPDGMLLRSYWWATNLSEPHNKYQFTRYFEERNQEPPDPLSRETFIDYA
                     LWFQKQVVPDVDETYVASIEREARHFIVTLEDGRIVQSQAVVMAPGLAYYVYRAPAYD
                     HLPAMLVSHTADHATFAPFAGKRVAVIGGGQSALETAALLHESGAEVDIIARRSVRWL
                     AGDSMKDRTLIKRMRYPKAGIAPGWFNWGLEKLPYTFQQLPRATKDRLLLGRGQYGPA
                     GSHWLKPRILGQVTLREQRQVEQINETDDGVSLTLSDNEIVKADHIILGTGYRVNINK
                     LPMLAPSLLSEVQTYHDAPVLTNHFESNVSGLYFIGISSLSSCGPLFRFVVGTDAAAR
                     RVAGAASRQVVRTK"
     gene            10334..11821
                     /locus_tag="VGT44_07380"
     CDS             10334..11821
                     /locus_tag="VGT44_07380"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEV2580660.1"
                     /translation="MQSPLKRYLALGRRWLWLVALAVVLCGGAGYVVTKLMKPVYQAS
                     AFVVVNLSTTTVPYDVNGSLAITPTYAQLMVSPAVLDQVVAQHPGLTLTQLSSMISVK
                     PQSNTQTIELDVQNDDPVLAAQLANEISKSLNDFANSRLSGGVQVLPADVPTTPIGPK
                     GSLNAAIGALVGLALAVAIIVLFEWFDDYLRDPEELQDLLGLDTLSVIPRLSRRQMSM
                     SVEEIPAPAEGCRILCANLNAAQSSQPFKLVMVASALPAEGRTTVATNMAVFLAMSGK
                     QVLLVDADLRRPGLHEHFRLDKRYGLASILLNLWEEDDTELDGQTTSFENLRVLPAGV
                     LSSNPGELLQLPEARRIFEHFEESQFDYVIFDTPPLLPVADAQVLASYVHTLVLVVDA
                     SKTPRKALVRAGKALSRAHANVLGAVINKSRWAEDSTISAYLKSVALRQSKVGVSRKK
                     APMSAPPNTPPVNGMVNHDAAHELVDAATITLARPQKDKKEDGEK"
     gene            11832..13133
                     /locus_tag="VGT44_07385"
     CDS             11832..13133
                     /locus_tag="VGT44_07385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV2580661.1"
                     /translation="MHQQLFEKIANRSLRVAVIGLGYVGLPLAITFAQAGYYVEGIDL
                     DKQKIDQANRGESYIPDIPGSALQALLATERIHFTSDFAVLEHIDAISICVPTPLRKT
                     RDPDISYIVSATQAVQAHLHAGQLIVLESTTYPGTTNEVVLPALESTGMQAGVDFFLA
                     FSPERIDPGNPQFGTHNTPKIVGGVTQTCTELAQAFYAAAIEKVIPLSSARVAEMAKL
                     LENTFRAVNIGLVNEIAIICDKLGINVWEVIEAAATKPFGFMPFFPGPGLGGHCIPVD
                     PHYLSWKLKTLDYTARFIELATEVNSSMPHYVVDKIGQALNEQRRCFNGATVLVLGVA
                     YKSNVGDVRESPALDIIQELIERKAVVIYNDEYVPDLHLPGHALSSQPLSSGLLQAAD
                     CVAIVTDHSYYDIAWIVREARCIVDTRNATRGRVAENVHLL"
     gene            13223..16360
                     /gene="murJ"
                     /locus_tag="VGT44_07390"
     CDS             13223..16360
                     /gene="murJ"
                     /locus_tag="VGT44_07390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEV2580662.1"
                     /translation="MPEPFIDPDMTQPMLSRSAYMAAVRLGSPKKNIFRALLSLASAA
                     LLIRLMGLVYQVIVTGHFGLGDAMDAYFIASLTPSLVAQLIASAVEYSVVPVYIRVRS
                     QQGRKQASELFSTLLNILLVGTLLLTVLMFLFRNQVVFLSAPALDSSRANLAAALMLY
                     IVPILVLQVIIALLESILNAEGQFGWPAYAGMLVPLTTAVFVLVFSKQLGIVALCLGA
                     VTGLCLQLVVFIIRVRQAKLVYKPRINMRNPAVMSVLVIAGPALLTAMIIQLGPLVDQ
                     IFASSLSAGSISALNYALKLNSVPVGIIFVSVGRAVVPYLSFQATARDMKAFKETLRL
                     YLWAVVITTIALSAFMTLLAHPIVQILFQRGAFSAADTTLTARTLIGFTLGLTPMAFG
                     FILSKAFNALGKTRVLMFVTVVSVSANAIFDFFFARLWQSTGIALATSAVYCITMVIL
                     LIALRREIGKLHLLTPPPEIIKVILSLGPGKNALREAAPGQANPPSFEIPTFFEIPFN
                     WRQPLVRAAVIVTAFIAALVGIYQNTLYALRYTVGAVIILLLMRYPYFLLIAWALINA
                     FIGSNLSIFNGNNLLTALTVPTLLLLVAVPMKQTLKRMPALILLILFLVWVFAGIRIS
                     PLGAVPFLTLWLNFVDFAALSVLTINIITTRRRLLGLIDAILIPAIFIALYGIYGYLT
                     KQYGVIDSTGVFRAYSIYNAAPALALLLSIMVPFAIYRTYTMRGILRLIGLAVTLVLL
                     VGVGVTFSRGAFISLPIGIIVMICFLPSPKLRIAMLGGFATVAAVLILLATVGNIPIF
                     DRFLLQNISSFNGRTYLWQALLQNFDPTQLLGHGLLASNAYLATLSVSNIATAASNLF
                     VGTLYDHGIIGVILLSLIFIALLVGLIGGIFKASGERRVLFAAALAALICMLFQSLES
                     DDFWIQAIAVYFWIFMALPFALFWSPPKQLPKLSTEDKEAVVVDRTIKTIAQRPAGAR
                     TIKAIAGRPDKTGIEQAEREMREGAVKTAVNPISAEMETIDDESEEARDVATTPRIMA
                     VKKETIEHTSTSPDA"
     gene            16353..17864
                     /locus_tag="VGT44_07395"
     CDS             16353..17864
                     /locus_tag="VGT44_07395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2580663.1"
                     /translation="MRSAGTVPVRACLVSFQFWPRVGGAEAQAEKQARQMQALGHEVI
                     VVTLRHEKRWQRIDVVNGLQVVRVGGIYKRSGTLRLGRLGHVPIDIGMFFTLWRLRRR
                     YDIMHVFQVSPVAAVAALIGQLAHKPVVVSVQSTGPGDAQRARIEQGVTLMADTLESP
                     GASDNKYSKPSSVIGCHPERSEGSGSPGTEILRCTQDDIPDGGRENLSSGERSDLSFL
                     NIDFIDVASASGDLSYLPQTAFGGNVILKYLRNSDAFYQVLSHRGYPYLTSRGFRAKQ
                     IVRISNGIDASRYHPEPALRPDSAGPDRDIVCVARMDYAKGVDVLVHAWSRMMRAPAE
                     WRAELRPRLRLVGDGVLMRQMQSIVTELGIQDSVEFLGLRADVVDLLRQAWAFVLPSR
                     WEGMPNALLEAMACGLPCVATRVSGSEDVIANGINGLLVEPEQPAEMALALRRVIEDT
                     ALAHQLGQQAVATVLREYQLPTIVEQCLSLYRCLLAKEKRALPLGLERNEIGR"
     gene            17854..19119
                     /locus_tag="VGT44_07400"
     CDS             17854..19119
                     /locus_tag="VGT44_07400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2580664.1"
                     /translation="MAGNPRIYIAISNFLPIVGGAEMQALAQARNLRARGYDSTVITL
                     RHDRAWPKCDEIEGVPVLRVGGRLVAGRERLPGALKKLAYLAGMLMLAWTLWRRRGRY
                     DILHVFQLNLLAVSTALACRLTGKPMIVAVRSTSTIEVEGTETHSEVGGDLAPLEALG
                     KPVVRLARALLRRNHAVVVVLSTRMRSYLAAHDFKLPDVRLIPNGVDVSRFTPPRGAT
                     TADRAHCVVCVSKLRYEKGIDVLLQAWRQVQAQLSPPGSARLVIVGDGPLRAELERLA
                     GELDIADSVEFAGLQSDVPAQLYRGGLAVLPSRWEGMPNALLEAMACGLACVATRVSG
                     SEDIIQHGINGLLVEPGDNEGMAQALLTLLRDSALAGVYGRAARETVEQQYAFERIMN
                     TYVELYRSLVGETSVKAEDTSVSEMSHVL"
     gene            19201..21102
                     /gene="asnB"
                     /locus_tag="VGT44_07405"
     CDS             19201..21102
                     /gene="asnB"
                     /locus_tag="VGT44_07405"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEV2580665.1"
                     /translation="MCGIAGFVDAEIGLNQAEQLIDGMCQVIRHRGPDDQGTWVGDGA
                     ALGMRRLSIIDVSGGNQPIFNEDHSILIVFNGEIYNYLELRDELQARGHHFATNSDTE
                     AIVHAYEEYGDDCVKRLRGMFTFAIWDRKRHRLLAARDRFGKKPLNYYWDGQRLIFGS
                     EIKSILQAGIPREVNPIALDEYLVYRYVPTPNTLFKNVMKLPAAHILIYEHGQVSTRR
                     YWELPFTPTCQDDEATASERILELLKDAVKVRLMSEVPLGAFLSGGVDSSVVVGLMSQ
                     MMSQPVKTFSIGFEEDYYSELPYARQIAQHFDTEHHEFFVRPELISVLPQLVWAYDEP
                     FADASMLPTYYVSKLAREYVTVALTGDGGDEIFGGYVHYERELMTSRIPLLMRLLLGY
                     GSLLMPNGMRGKKRFHNMLYDHARRNVCSTTLFPENSRHAMYSRDYFERVCDHDPFER
                     LLSQYRNVPNLDVTASMQYVDARVYLTDDILVKVDKASMFNSLETRAPLLDQHLVEYV
                     ASLPSSLRLQNGVLKYLLKKVALDMVPAETVNRPKHGFSVPIKQWFRGDLFAYARELL
                     ESPRAQQRGIFNPQFVSNMLKTHASTKLVNFSEAIWALVCLELWFQVYIDEPPLRVVE
                     TPQIQVANH"
     gene            21115..>22125
                     /locus_tag="VGT44_07410"
     CDS             21115..>22125
                     /locus_tag="VGT44_07410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2580666.1"
                     /translation="MDDPILPRDARKPYRVLMVTGIYPTAARPDKGTYIKSQVESLIA
                     AGLEVEVIHPKPGPAPLRYASAYLQVFLKTLTRKFDVVHGHYGIWCLTARLQWTTPVV
                     ASFLGSDLLGVVTADGGFSSFGERVARISRWLCRHVDAVIVKSEEMKNAVQGKGSIFI
                     IPNGVDFDLFRPRPRAQVRAELGWDADRSYVLFGNDPRRTVKNFPLAQAAFERLHTRG
                     VDAELVVANGLPHEQVAHYINASNAVLLTSFTEGSPNIVKEAMACNVPVVSTNVGDVA
                     QLISDTDGCAVCPGDPDALADALVVALHHTEPTTGRSNIQHLDRSAVAPQVIAVYEQV
                     IKK"
BASE COUNT         4503 a         6364 c         6354 g         4904 t
ORIGIN      
        1 acaagggctg aaagcgaatc ccgactccct ggatttgatc gtcatgctga taacgcttta
       61 cctgcaacaa gaggatatag cggggcttga acgcgccatc aaacagttag cccgcctaga
      121 cccacattat cctgcgcttc ctttactgcg ctctatgctg cccacgttga aagccacttt
      181 cagatctgga gcctcaaaaa cgggtatgcc gcctttggtc ttagcgccga tgccggaacg
      241 cccaaagaag agacgttagt cacgggtcgt atcgaggcac gcgcgcaaag tctcgaccag
      301 ggtggggagt tgatcggtaa caaggcccat atgcttgccg ggaatgacgt gaatctctac
      361 attttgcgtt ttctccacgt gcagccagcc gttccggaac ggctctttcg cagaccagtc
      421 cgcgctcgac cagaaaaacg tgatctttcc aggatacaat ctcggtggtt tataccccag
      481 cgtcaaccag gtaaaaacgc tttcataatc ctgccgcagc gtcccggctt tcggtagcca
      541 ttcgctgggg cggggaaaag tgacgctgcg ctggccccag cgctcgatct gttcggcagt
      601 tcctagatgc agggcagcct gcaattttct gatgcgaatg cggaaataat ccttcacatg
      661 taggaagtac aggcgagcgc gccgtatgcg cacataccaa tcgatttgct tctcttctcc
      721 gctgcgcaat agcttgccca gcaagacgag ggcatggcgc gtcatgtggt aacgccgggg
      781 gtaaacgagc gccatcgaat tcatcaagac cagcaggtca acctgctggc ctgcctcgtg
      841 aagctggcgc gcgatttcat aggccatcag cgcgccattg caccagccac tcaggagata
      901 aggcccttcc ggctgcacag cgcgtatggc cttgatatgg attgccgcga tttcctgaaa
      961 agacggggga acgggttgtt gctcgaaggt aaatggctct accacgtaga acggctgatc
     1021 ggaacccagg ccctgcgcca gggggaagca ataaaacgcc atgctttgcc actggccatg
     1081 caggaagaaa aatggccgct ttgatcccct ggcctggatt ggcacgacgc gcgggccggt
     1141 cgattcggct tgcgcctccg ggggcgcggt atccctggta tcggcctgtg ttgagagttc
     1201 cccgcgcaaa tacttttcga gtaatgcgcg cttggcgtct gaaagttccg gcattgttcc
     1261 cttattatct tgactaaaat gagacatgca gggccgtcag cccgcgcagc cccaggtttt
     1321 ctcgccaggt gatcggacct ggatcgagac gcaggtcggg cattcgttcc agcacggttt
     1381 tgaaagctat ctgcccttca atacgcgcca gtggtgcgcc gaaacagaaa tgactggccc
     1441 aggcaaaagc cacgtgccgg ttatcctgcc gctgaaaatc gagccgatcc gggtcgggaa
     1501 atcgctccgg atcgcggttc gccgcgccca tcacagcgat gaccgcctgg cgtttgcgga
     1561 tcgctttgcc gccaagctca gtatcgtctg gagccaggcg cgccgtctgc tggctgggac
     1621 tctcataacg caatagttcc tcgactgcgg aagggatcag tgaaagatcg gtcttgagtt
     1681 gctcgaactc ctcgggatgg cgcagtaggg tgaggatgcc attgccgatc aggttggtgg
     1741 tcgtttcctg gccgccgacc atcgtcacaa ttgtgttagc gataacctca tcttcggtca
     1801 ggcggtcgcc gtcgatctca gcggtcacga gcgcgtggat caggccatcg cgcgggtgcg
     1861 cctgctgttc gtggatagct gcgcggaagt agtcgaccat cccttccagg ctctggatga
     1921 cctgcgccgc gtgttcggga ttatgctgga aattccccag caccccggca aagtccgcgg
     1981 accacttcgt caactgctgc cagtccgtgg taggcacgcc cagcatctcg gcggtgacga
     2041 ttgcgggcag agggatagcg aggtccgcca gcacattcat atggccctgc tgctgtacgg
     2101 cgtcaagcag gctgttcgtg atgtcctgga tatgcgagcg cagcacctct actctgcgcg
     2161 gagtgaaggc tttcgatgcc aggccgcgta ccctgccatg cgcaggtggg tcgagaaaca
     2221 gcatctggcg caccatgacg cgcgcaagcg gcgtcagcgc ctccaggccg agcgaggtca
     2281 actgttccgg ggtgggtgtg cggttggcgg agaaacgttg aaacaccgag accacgtcgg
     2341 cgtagcgcgt gactacccag gcatgcagga aggggtccca gtggacgggg tcttcggtgc
     2401 gcagccgttg gtataatgga tagggattgg ccagcacctc cgggtccaag aggtgataga
     2461 gactcaacgg cggttctttt tttcctggtg tttgtgatga ttgtgtggaa agcgtcatgc
     2521 tggttcctcc gtatgcatgg gtcctctggc tctattgaac ttgctcaagc agacgctgaa
     2581 catcttggtc gctcatcatc tccagcttta tgagaatcag tcgttcgatt tcttcggaca
     2641 attgccggat ggtgggagcc tcaaacaacg tgcggagaga gagatcgacg ccaaatgccc
     2701 cactgacgcg ggcgatgatc tgcgttccca gcaacgagtg gccgcccagc atgaagaagt
     2761 tatcatctct gccgatcgtc tccaggttga gcagcgtggc cacaatcgcg gccagacgtt
     2821 cttcgagggg cgtttccggc gcggtagctt cgccgtctcg cacgatgttg ccagaatcag
     2881 gcgcgggcag ggctgcgcgg tctatcttgc cgttgggcgt caccggcaga gcctccagcg
     2941 gcacgaatgc cgtcggaacc atgtattccg gcagttgcgc cgtcagcgtg tcccgcaggg
     3001 agccgagcga gacctgcgcc ccggcgtttg gcacgatgta ggccaccagc cgtttattgc
     3061 ccggcgtatc ttcacgcgcc accaccaggc tcgtctggat agcagggtga ctgttcagca
     3121 ccgccacaat ttcgctcggt tcaatgcgat agccccgaat tttgatctgg tgatcggcgc
     3181 gccctaaaaa ggcaacctgg ccatccggca gaaaataggc caggtccccg gttttgtaga
     3241 gacgcgcgtc cgcttcgctg ctgaacggat tcgcgacgaa gcgctcggcg gtcagatccg
     3301 ggcgattgag ataacccttg ccgagtccat gcccgcctat ataaagttcg cccgtttcgc
     3361 cgattggaac ctgtcgcatc tgttcatcca ggatgtaaag ttgtgtgttc gcgatggggc
     3421 gtccgatcga tggaggccgg tctgcgcgcg attccggcaa gatacgtccc gatgtattga
     3481 cgacgctggc ctcggtcagc ccgtaattgt tgatgaaggc gaaaggcagg ttgggtgatg
     3541 gatagcgctc aagcttgtcc gcgccagtca gcaagtagcg cagcgccgtc gtcgcgggcc
     3601 aatcaagcgc catcacgctt tcagccagcg ctgtcggcaa gaaagaaatc gtgatgccgt
     3661 tgcgtaccaa ccagtcgcgt agggttgcag gcgtgatgcg agtatcttcg tcgggcaggt
     3721 agacgcttgc cccgatagtg aggtatggcc acaattccca accggttgcg tcgaaggcag
     3781 ggctggcgac ttgcgtcgcg tggtcggcgg cggtgacgcc gaaggtgtgc tggtgccagt
     3841 acacgaggtt gagcaggccg ttatgcgtta ccaggctgcc cttgggtttg ccggttgagc
     3901 cagaggtgta gatgacgtag atgagatcat cagcagtgac atcggaacgt ggattgctcg
     3961 agtcatagcg tgccagcata tcggcgtcgg tatccaggca aaacatgcgg tcttgctcaa
     4021 tgggcagacg ttcggccagg taatgatgcg tgagcagcac agcagcctgg gaatcttcca
     4081 gcatataaat caggcgatcc gagggatagg ccgggtccat cggcacatac gcgccccccg
     4141 ccttcagaat tccaagcaat ccgatcacca tatccaccga gcgctccacg cacaggccga
     4201 caagcacatt agggcgcacc ccgtgacttt gtagatagtg cgcgatccgg ttggcgcgct
     4261 ggtttagctc caggtaggtt agcacctggc tgcccaccac cagcgcaacc gcgtcgggcg
     4321 ccgcggcggc ttgccttgcg accagcgctg gaacgcataa atctgttgaa tagtccagcc
     4381 gggtagcctc attccacgct gctaactgct gcgcttcctg ctctgtatta ttggtatcca
     4441 caatctctat ctcctgaact cggtattagt gacagatata gttgcaatca gtgtcttacg
     4501 aataaatctg atacggcaag ccagaatacg ggacactact tcactaagcg tacagcagtt
     4561 caatgccaat acggttgatg atggtgttaa gatggtattg aaataggcta attcctgtgt
     4621 atataacaat acctctcacg ctcctaccgg gaaaactcaa tatcaccaca gacttcacaa
     4681 cgcccgcgca gtctgtcctc ttgttcttgt acctccgcaa gccgccttgc gcagcgacag
     4741 acatagccgt gctggcgagc gggattgcca aagacaagcg cgtgcgcggc aacatcccgc
     4801 gtcaccattg acccggctcc gacgagggcg aattcgccaa tcgtaatccc gcacaggatc
     4861 accgctcccg cgccaatcga cgcgccgtac ttcacaagcg taggggtgat ctcccagtca
     4921 gcagcgcttt tgaacctgcc gtcgggcgta atggcgcggg ggagtagatc attcgtaaaa
     4981 cagacatgcg ggccgatgaa cacgccatct tccagcgtga ctccttcgaa gatagacacg
     5041 tggttctgaa ttttcacgtt tgagccgatt tgcgcgtgcg cgtcgatgta gacgcctttc
     5101 ccaatattgc aggatcgtcc aatgtgcgcg tgttctcgca cgtgcgcctg gccccagatg
     5161 cgcgtgcccg ctcccacctg cgcgtctggc gaaacttctg ccgttggatg aacataaaaa
     5221 gctggttcgg tcatagacta cttccacgat gttgttgtaa agtgctgctg gacaaacggg
     5281 accttacgca aacgggcgaa tagtccccac aggccagctg tttcaaccgc catgagtaac
     5341 cagtgttgta tggaagcaac ctctatacgc ttgagcaggt aggggtcggt ttccgttgta
     5401 ttgatgccat agatggtcgt gaccgcccag tcgtaccccg cctgttgtac gacgcgagtt
     5461 gtatcggctc ccaggtactg catttgacca ataggatagg ccatggcgcg tatcgggtgc
     5521 tccagttcct gctccagcgc ggcgcgactc tcctcaagct cgctcaatac ctcccgtgga
     5581 tcggccagcg ccgctaaaat gggatgatgc atcgtgtgcg cgccaaacga aacccaggcg
     5641 ctcttatcca tcgtgcgcgc ctcttcccac gtgagggaac gctcccactc gtccacttct
     5701 gtgtcaccct cggttatgga caatgtctgg cgaatcgttg tcagaaatgc ttcgcgctct
     5761 tgcaccgagg cagcgaagcg caggcgcgca tcgatggcac gcgccagctc ccgctgttcc
     5821 ctggcctgtc ccaggtggta aacacggcct gctagcgtta cttcatcgac tcgcgcatga
     5881 cgtacgagcc atttgctttc ccgccaccag aagcgctggc cgctctggac atagccagga
     5941 atgagaaaga tggtgatcgg cacctgcaac tcgctcgcca gcgcgaagcc gtgcgtgtag
     6001 ttgtctggat agccgtcatc gaaggtcagc gccagcagcg ttttttgctg cgtagcgttc
     6061 attttgccct tcaccagcga ggagagttcg accagcgccg cttcaaggtg taagatgcgg
     6121 taatgatggc gcaggtagag caggtggcgg cgcagatccc cgtcgcagac ccggtggtaa
     6181 ttgagaatca ccaggcgcgg tccgcactgc cggtgccacc accgcgccag tctcaccaat
     6241 ccgctgtaat acaggcaggc ggcaatgcaa agcaaaataa ctttacgcat gtgaaccctt
     6301 cctcctcaaa tgtcttacaa gaaggttcat gaacgcagta gttgctgtcc agacgggcag
     6361 cggatctcgc caatccaggt agtcgtaact cattggcttg aagaagctca gaaagaatgc
     6421 caggatcgcc tgtgtcgggg gagccactcc cggcctgccg cgctgcaaga cagcctcggc
     6481 cgttgtcagg aaatcgcctt ccaagtaacg catccaacct ccctcgcgat atcccttcac
     6541 cacgtcgatg cggtctccat tcgcccattg atacaacagg taggggaaat cgacgccggc
     6601 acgcactgcc acttcgatag aagcagagag gcgcggattg atctccatca ggtagggcgt
     6661 cccgtgattg tcgcgccggt actcgatctc ggagtagccc tccaggtcga ttgcgcgcac
     6721 taaacgctca gattgttcgc caatatcggg tggtatggcg atgctctgcc gcagcaccga
     6781 tgtcccaccc agttgtggat tcgtgcggct ggcccattgc gcgaaacgcg cgtaaaattc
     6841 gccgcgcgca tacagaaggc tgacagcctc gcgccgaccc gtcagaaact gctggaacag
     6901 cgtactgccg cccagcctgg tcaactcttc aacggcctgg cgcgcttcgt cggcgctggt
     6961 gacgagctta catgtcagcc tggcgctttg cagctcgccc catagccatg actcaaccgg
     7021 tttgaccacc gcgggcaggc caatctcctt cactgctgct gcaacatcct gcgccgagtc
     7081 caccttcacg ccgcgcggga tggcgatgcc cagttgagcg gcaattgcta aggtctggtc
     7141 tttgttgatg gcgattgcca gtgcagggtc ctttgccagc gcgatagcga cgcgcttctc
     7201 cagttgctcg cggtgctgat gtaccagagc gagtgtgccg tctgaggaag taatcagcac
     7261 gcgcgcgcgc gtttgatcga gaacctcttc caggaagcgc agatagggtt cggtgctttg
     7321 cacatacgat ggggcgatat acttttgtcg gttccagcgc gaagagaagg tgggcgtcgt
     7381 gcctacgctg ttcaacttgt tgcccgcctc aagtgaggcg acacgcagcc cccggcttcc
     7441 cagcgagcgc acagagacga gggattgtcg cagaattgcg tccagcacga gcgcatcgta
     7501 ggctgctgcg ggttttacaa gcgctgttgc gagtcccgct cgctcatcag cgcctgcaac
     7561 gggtaattga gcgttttcgt caaaagggag aggtactgtc tctttcatat aaataatcgc
     7621 tcctgacttc acttcgccac ttgacgcaaa ttcaaatagt tattcacgcg cgatacaatg
     7681 cccagcaacc cgaccagttc tgatgccatg accagccagt gtttctccac cacccctggc
     7741 agtcgccgca agagatactg gtcacactgc ggggtattga cctcctcgca agttgtaact
     7801 gcccaggtat accctgccga tttcaccgcc tggacacccc gctcgccgaa atgctccggt
     7861 ttgccgatag gataggcgaa ggcgcgcacc tggtggccca gttcttgctc caggacacgg
     7921 cggcactcct caacctcgcg tcggatctcc agcgggtcgg ccagcgccgc gaggacggga
     7981 tgatgcatcg tgtgcgcgcc aaacgagacc caggcgctcg cgtccatctc acgcgcctct
     8041 gtccacgtca tggggcgttc gccttcttca cccggtatga cctcgtcgcg tatccgcagc
     8101 gcctggcgaa cttcgcgcag caatgcctcg cgctgctcta ccgaggtggc ggagcgcagg
     8161 cgtgtgtcga tggcatgtgc cagcgcgtca cgctcccccg gttggctcag gcgatagatg
     8221 cgcccatcga tggttgcctc gtcggtggtc acgctggcgg cgagccgctt gccttccagc
     8281 caccagaagc gttcgccgct ccgaatgtag ccaggtacga ggaagatagt catgggaacc
     8341 tgcaatgctt gcgccagagg gaaggcatga gtgtagttgt cgcggtagcc gtcatcgaag
     8401 gtcagcacga gcggcatgcg gcggtcattc agccgcttgt taggaggttg tgcgccatag
     8461 agttcttcca gcgcggcctc gaggtggagc aggcggtagt ggcggcgcag gtagagcagg
     8521 tgatcgcgca agttactcgc gctggcctgg tgatagttca gaatcaccag gcgtggcccg
     8581 ccgcgctgca accgccagta ggctagtttc accaggccac tatagtagaa acacgccgcg
     8641 atccacaggc gcacgcgctc gcgcatatga ctccttccct ccttgatctc ccacgttgaa
     8701 catagacatt atacgctgga cgcgagctta ctttgtacga acaacctggc gcgatgcagc
     8761 gccggcgacc cggcgcgcag cggcatctgt gccaacgaca aatcggaaga gcgggccgca
     8821 gctcgacagg gaagaaatgc cgataaagta cagacctgac acattgctct caaagtggtt
     8881 ggtcaagaca ggtgcatcat ggtacgtctg cacttcggat agcagcgacg gggcgagcat
     8941 gggcagcttg ttgatgttta cgcgatagcc ggttccaagt atgatatggt ctgccttcac
     9001 gatctcgttg tcggagagcg tcagcgacac accatcatcc gtctcgttga tctgctcgac
     9061 ttgccgctgt tcgcggagtg tgacctgacc gagaatgcgc ggctttaacc agtgggaacc
     9121 ggcgggtcca tattggccgc gaccgagcag cagccggtct ttcgtagcac gcggcagttg
     9181 ttggaaggta tagggcagtt tttccagccc ccagttgaac catccaggag caatgccggc
     9241 ttttggatag cgcatgcgct tgatgagtgt gcgatccttc atcgagtcac ccgcgagcca
     9301 gcgcacggag cggcgcgcaa tgatatcgac ctcagcgccg ctctcgtgta gcagcgccgc
     9361 cgtttccagc gcgctttgcc cgccgccgat gacggccact cttttgcccg cgaatggggc
     9421 gaacgtcgcg tggtctgctg tgtgcgaaac cagcattgcc ggtaggtggt catacgcggg
     9481 ggcgcgatag acgtagtagg ccaggccggg cgccatcacc accgcctggc tctgcacgat
     9541 ccggccatcc tccagcgtca caatgaaatg gcgcgcttcg cgttcgatgg aggcgacgta
     9601 ggtttcgtct acgtcgggaa caacctgttt ttgaaaccac agcgcgtaat caatgaaggt
     9661 ttcgcgtgag agtggatcgg gcggctcctg gttacgctcc tcgaagtagc gtgtaaactg
     9721 gtatttgttg tggggttcgg ataggtttgt cgcccaccag taggaacgca gcagcatacc
     9781 atcgggcatg tgttcgcgcc agagcttcaa gggcttccca aaaatcgcca cgttcagccc
     9841 acgctctcgc aagtgcgcgg tcgtcgaaag tccataaggc cccgcgccga cgacaaccac
     9901 gtcgcgcaga tcagtggata gcggaggtcg cggctttttt ctagccatcg atatattcaa
     9961 taacgatctc ccaaaagatg acgttcatgt tggcacagta tcccggcgtt gtagtcctgc
    10021 atctgacaaa acacctcaat cctcacctgc aaacaagggc gtaaataccc cctgtaacaa
    10081 gataacatat ggtagtgttg aatcgatgat gagcggggta aagaatgagt aacgctgtag
    10141 ggcgctctgt gcagaaaagc ttatcctgaa cggttctgca aggattagga acgtaaacca
    10201 ggggttggtg ttcaccacgc tatctcaacg tttccagcaa ccagaaactc aagggaattg
    10261 cggtactgta gaatgggata ttaataagat agattcgcta ctcttaaatt caagaggtaa
    10321 ggatggtcag gatatgcaaa gtccgttgaa acgttatctg gcgctcggca ggcgctggtt
    10381 atggttagtg gcgcttgccg tcgtgctgtg cggcggtgca ggctatgtag tcacgaagtt
    10441 gatgaagcca gtctaccagg ccagcgcttt cgtggtggtc aacctcagta ccacaactgt
    10501 tccttatgat gtcaatggca gcctggcaat cacgccgacc tatgcgcagt tgatggtaag
    10561 cccggccgta ctcgaccagg tcgttgcaca acaccccggc ttgaccctga ctcaactgtc
    10621 cagcatgatc tcggtaaagc ctcaatcaaa cacacagacc atcgaacttg atgtgcagaa
    10681 cgacgatcct gtgctcgccg ctcaacttgc caatgaaatt agtaagagcc tgaatgattt
    10741 tgcgaattca cgcctttccg gtggagtcca ggtcctgcct gctgatgttc caacaacacc
    10801 tatcggcccc aaaggctcgc tcaatgcagc catcggagcg ctggttggtc ttgcgctggc
    10861 cgtcgcgatc atcgttctat ttgaatggtt cgatgattac ctgcgcgatc ctgaagagtt
    10921 gcaagacctg cttggccttg ataccctgtc cgttatcccg cgtctctcgc gcaggcagat
    10981 gtcgatgagc gtcgaggaga tacctgcccc ggcagaaggc tgtcgcattc tgtgcgcgaa
    11041 cctgaacgcc gcgcagtcct cccagccgtt caagctcgtc atggtggcca gcgcgctccc
    11101 cgctgaggga agaacaaccg tcgctacgaa catggcggtc ttcctggcta tgtcgggaaa
    11161 gcaggtgctg ctggttgacg cggacttgcg ccgtcccggt ctccacgagc atttcaggct
    11221 tgacaagcgc tatgggttgg cgagcattct gttgaattta tgggaggaag acgacacgga
    11281 actcgatggg cagacaacca gctttgagaa cctgcgtgtc ttgcccgccg gtgtcctctc
    11341 ctctaaccca ggtgaactac tccagttgcc ggaggcgcgt agaattttcg agcattttga
    11401 agaatcccag ttcgattatg tcattttcga tactccacct ctgctgcctg tcgccgacgc
    11461 acaggtactg gcatcctatg tacataccct tgtgctggtt gtagatgcct cgaagactcc
    11521 tcgtaaagca ctggtacgcg ccggaaaggc gttgagtaga gcgcatgcaa acgtactggg
    11581 tgcggtcatc aataagagtc gctgggcaga agatagcacc atcagcgctt acttgaagag
    11641 cgtggctctg cggcagtcga aagtaggtgt cagcaggaag aaggctccta tgagcgcgcc
    11701 tcccaatacc ccgccggtca atggcatggt caatcacgat gcggctcacg aactggttga
    11761 tgccgccacg attacccttg ctcgcccgca aaaggataaa aaagaagacg gggagaagta
    11821 ggagcctttg aatgcaccag cagttgtttg agaaaatcgc aaaccggtct ttgcgcgttg
    11881 ccgtaattgg cctggggtac gtcggtttgc ccctggccat cacgtttgcg caggcgggct
    11941 actatgtcga gggcatcgac ctggacaagc aaaaaatcga ccaggccaat cgcggggaaa
    12001 gttatattcc tgatattcca ggtagtgctc ttcaggcgtt gctcgctacc gagcgcatcc
    12061 atttcaccag cgatttcgcc gtactagaac atatcgacgc catcagtatc tgcgtaccca
    12121 ccccgctgcg caaaacacgc gaccccgata tctcctatat cgtttcggcc acgcaagcgg
    12181 tgcaggcgca tctacacgct ggacaactga tcgtattgga aagtacgacc tatccaggca
    12241 cgacgaacga ggtggtgctg ccggctttgg agtccaccgg gatgcaagcg ggcgtggatt
    12301 tcttcctggc gttttcaccg gagcgcatcg accccggcaa tcctcagttt ggcacccaca
    12361 atacgccgaa aatcgtgggc ggcgtgacgc agacctgcac cgaactggcg caagccttct
    12421 acgcagcggc catcgaaaag gtcattccgc tctcgtctgc gcgcgttgcc gagatggcaa
    12481 agctgctgga gaacacgttc cgcgccgtca acatcggcct ggtcaacgaa attgccatta
    12541 tctgcgataa actgggcatt aacgtctggg aggtgattga ggctgctgcg accaaacctt
    12601 tcggcttcat gcccttcttt cccggcccgg gcctgggagg acactgcatc ccggtcgacc
    12661 cgcattatct ttcctggaag ctcaaaacac tcgactacac ggctcgtttc atcgaactgg
    12721 caaccgaggt taacagtagt atgccgcatt acgtcgtgga taaaatcggc caggcgctaa
    12781 atgagcagcg ccgttgcttc aatggcgcga ccgtgcttgt gctgggagtt gcctacaaaa
    12841 gcaacgtggg cgatgtgcgc gagtctcctg cccttgatat tatccaggaa ctgatcgaac
    12901 gcaaagcggt ggtcatctac aacgatgaat acgtgccaga cttgcatttg ccgggtcacg
    12961 cgctcagctc ccaaccactg agtagcggcc tgctgcaggc cgctgactgt gtggccatcg
    13021 tgaccgacca cagctattat gatatcgcct ggattgtgcg tgaagcgcgt tgcatcgtcg
    13081 atacacgcaa tgcaacacgg ggacgcgtcg ctgagaacgt gcatttactg tgaaaatgga
    13141 aaaagttccg agcgattgct gcaaacaatg ttgttacaga gatgtttatt gctttctgtt
    13201 tttttaaaga acggtcatag aaatgcctga gccatttatc gatcccgata tgacccagcc
    13261 tatgctctcc cggagcgcat acatggcggc agtccgcctg ggttctccaa aaaagaacat
    13321 ctttcgcgcg ctgctgagcc ttgcgtctgc ggccctgttg attcgactga tgggcctggt
    13381 ctaccaggta attgtgaccg gccatttcgg tctaggggat gcgatggatg cttattttat
    13441 cgcctccctg actccttcac tggttgcgca gttgatcgca agcgcggttg aatactcagt
    13501 cgtacccgtc tacattcgtg tgcgttctca gcaaggaaga aaacaggcat ctgaactatt
    13561 cagcaccctg ctgaacatct tactggtggg gacgctgctg ttgacggtac tcatgttcct
    13621 ctttcgtaac caggtcgtgt tcctgtccgc cccggcgctg gattcatcgc gggcgaacct
    13681 ggctgccgcc ttgatgcttt atatcgttcc gatcctggtg ttgcaggtta tcattgccct
    13741 cctggaaagt attttgaatg cggaaggtca gtttggctgg ccggcctacg cgggcatgct
    13801 ggtgccgctc accaccgctg tatttgtgct ggtgttcagc aagcaactcg gcatcgttgc
    13861 cctgtgcctg ggggcagtga ccggcctgtg cctccaactt gtcgtcttca tcatacgtgt
    13921 acggcaggcg aaactggtct ataagccacg catcaatatg cggaaccctg ctgtcatgtc
    13981 ggtgctggtc atcgccgggc ctgccctgct gacagccatg attattcagc ttggcccgct
    14041 tgtcgatcag atatttgcct cgtcgctctc ggctggcagt atttccgcgc tgaactacgc
    14101 gctcaagctg aatagcgtcc cggtcggcat catctttgtt tcggtcgggc gcgcggtcgt
    14161 cccgtatctt tcctttcaag cgactgccag ggatatgaaa gccttcaagg aaacactgcg
    14221 cctctatctc tgggctgttg tgatcaccac gattgctttg agcgctttca tgactctgct
    14281 ggcccatccc attgtgcaaa ttctctttca acggggggcc ttttctgccg cagataccac
    14341 ccttaccgcc agaacgctga tcgggttcac gctgggcctg acgccgatgg catttggctt
    14401 tatcctgtcg aaagccttca acgccctggg caaaacccgt gtattgatgt ttgtgaccgt
    14461 tgtgagcgtc agcgctaatg ccatttttga ctttttcttc gcgcgcctgt ggcagagtac
    14521 cggcattgcc ctggctacct cggcggtcta ctgcatcacg atggtgattc tgctcatcgc
    14581 gctgcgacgc gagatcggca aactccacct gttgacacca ccacccgaga tcatcaaagt
    14641 gatcttgagc ctgggtccgg gtaaaaacgc tctacgcgaa gccgcaccgg gacaagcgaa
    14701 tcctccctcg tttgagattc ccaccttttt cgagattccc tttaactggc ggcaaccgct
    14761 tgtgcgcgcc gccgttattg tgaccgcctt tattgccgcg ctggtcggta tctaccagaa
    14821 taccctctat gcgctgcgat ataccgtcgg tgctgtgatc atcttactcc tcatgcgtta
    14881 cccgtatttc ttgctgattg cctgggcgct catcaatgcc tttattggct cgaacctctc
    14941 gatcttcaac gggaacaact tgcttacggc cctgactgtg ccgaccttgc tcttgctggt
    15001 cgctgtaccg atgaagcaga cgctcaaacg tatgccagcg ctcattctcc tcatactttt
    15061 cctggtatgg gtcttcgccg gtattcgtat atccccactt ggcgccgtgc cgtttctgac
    15121 gctctggctc aacttcgtcg atttcgcggc cttgagtgtg ctgacaatca atatcatcac
    15181 tacgcgcagg cgtctgctcg ggctgatcga cgctatattg ataccggcca tctttattgc
    15241 gctctatggc atttatggct atctgacgaa acagtatggc gtaatcgact ctaccggtgt
    15301 gttccgcgcg tactctattt ataatgccgc gccggcgctg gctttgctgc tctccatcat
    15361 ggttccgttt gccatctatc gcacatatac catgcgtggc atcctgcgcc tcatcggact
    15421 agcggtcacg ctggttctcc tggtaggagt cggcgtgacc ttctcgcgcg gcgcatttat
    15481 cagtttgccg ataggcatca tcgtcatgat ctgtttcctc ccttcgccaa aattgaggat
    15541 cgctatgctt ggcggctttg caacggttgc cgcggttcta atcctactgg cgaccgtagg
    15601 aaatattcca atatttgatc gcttcttatt gcaaaatatc tcgtcattca atggacgcac
    15661 ctatctctgg caggcgctcc ttcagaattt tgacccgacg cagttgctcg gtcacggctt
    15721 gctcgcatcc aatgcctacc ttgccaccct gagtgtgagc aacattgcga ctgcggcctc
    15781 caatctcttt gtcggcaccc tgtacgatca tggcatcatc ggagtcatct tgctctcgtt
    15841 gatcttcatt gccttgttag ttggtctgat cggcggcatc tttaaggcca gtggagaacg
    15901 tcgcgtcctc tttgccgcgg cattagccgc cttaatctgt atgctctttc aatcgttaga
    15961 atcggatgat ttctggattc aggccatcgc cgtctacttc tggatcttta tggcgttgcc
    16021 gttcgcgctt ttctggtcgc ctcctaaaca gttgcccaaa ctgtccactg aggacaagga
    16081 ggcagtggta gtcgatcgga ctatcaagac gatagctcag agaccagccg gcgcgagaac
    16141 gatcaaagcc atagctggca gaccggataa gacgggcatt gagcaagcag agagagaaat
    16201 gcgtgaagga gcagtcaaaa cggctgtcaa tccaataagc gccgaaatgg aaacaatcga
    16261 cgatgagagt gaggaagcgc gtgatgtggc aactacgccg cgtatcatgg cggtcaaaaa
    16321 ggaaacaatt gaacatactt caaccagtcc cgatgcgtag tgccggcact gtccctgtgc
    16381 gcgcttgcct ggtctccttt cagttctggc cgagagtagg cggcgcggaa gcccaggcgg
    16441 agaaacaggc tcgtcagatg caggcgctcg gtcacgaggt gatcgtcgtc acgctgcggc
    16501 atgaaaaacg atggcagcgc atagacgtcg tcaatggctt gcaggtggtg cgtgtgggcg
    16561 gcatttacaa gcgctctgga acgctgcgcc tgggcaggct ggggcacgtg ccgatagaca
    16621 tcggcatgtt cttcaccctg tggcggctgc gtcggcgcta cgatatcatg catgtctttc
    16681 aggtttctcc tgtcgcggca gtggcggcgc tcatcgggca acttgcgcat aaaccggtcg
    16741 tcgtcagcgt gcagagtacc ggaccaggcg atgcccagcg cgcgcgcatt gagcaagggg
    16801 ttacgcttat ggccgataca ctcgaatcgc ccggtgcctc tgataataaa tattcaaaac
    16861 caagctctgt cataggttgt catcctgagc gcagcgaagg atctggctcg ccgggtaccg
    16921 agatccttcg ctgcactcag gatgacattc ccgatggtgg tcgtgaaaac ttatcttcgg
    16981 gggagaggtc tgacctgagc ttcttgaaca tcgacttcat cgatgttgcc tcggcctctg
    17041 gcgacctgag ctatttgccc caaacggcct ttggcggcaa tgtcatcctg aaatatttac
    17101 ggaattcaga tgcgttttac caggttttaa gccatcgcgg ctacccctat ctcacctcgc
    17161 ggggatttcg cgcgaaacaa attgtgcgca tctcaaacgg tatcgatgcg tcgcgctatc
    17221 accctgagcc agctcttcga ccagactctg ccgggccgga tcgcgatatt gtctgcgtag
    17281 ctcgtatgga ttacgccaag ggagtggacg tgctggtcca tgcctggagt cgcatgatgc
    17341 gcgcgcctgc tgaatggcgc gctgagctgc ggccccggct tcgcctggta ggagatggcg
    17401 tcctgatgcg gcagatgcag agtatcgtaa cggaacttgg tatccaggac agcgtggagt
    17461 tcctgggttt gcgcgccgat gtggttgatc ttttacggca ggcatgggct ttcgtgttgc
    17521 cttctcgctg ggaggggatg cccaatgcgc tcctggaggc catggcgtgt ggtctgccct
    17581 gtgtggccac gcgggtcagt ggtagtgagg atgtgatcgc caatggcatc aatggcctgc
    17641 tggtcgaacc ggagcagccc gccgagatgg cgctggcgct gcgccgcgtc attgaagaca
    17701 cagccctggc acatcaactc ggccagcaag ccgtcgcaac ggtactgcgc gagtatcagc
    17761 tgccgaccat cgtagagcag tgcctgagcc tctatcgctg tctcctcgca aaggagaaga
    17821 gggcgttgcc tcttggcctg gaaaggaacg aaattggccg gtaatcctcg catctacatt
    17881 gcgatttcta actttcttcc aatcgttggc ggagcggaaa tgcaggcgct cgcgcaagcg
    17941 cgcaacttgc gtgcgagagg ctacgactcg accgtcatca cgctgcgcca cgaccgcgca
    18001 tggccgaaat gcgatgagat cgagggtgtg ccggtcctgc gggtcggcgg gagactggtg
    18061 gcgggccggg aacggcttcc cggcgcgctg aaaaagctgg cctacctcgc ggggatgctc
    18121 atgctggcct ggacgctctg gcgtcggcgt gggcgttacg atatcttgca tgtcttccag
    18181 ctgaatctcc tggccgtgtc gacggcgctc gcctgtcgat tgaccggcaa acccatgatc
    18241 gtcgccgtgc gcagcacaag cacgatcgag gtggagggaa cggagacgca cagcgaggta
    18301 ggcggcgact tagcgcccct ggaagccctg ggcaagccgg tggtgcgcct cgcgcgcgcg
    18361 ctgctacggc gcaatcacgc ggtcgtggtc gtcctcagca cacgtatgag gagctatctt
    18421 gccgcccacg atttcaaact tccagacgtg cgactcattc ccaacggcgt cgatgtctcg
    18481 cgctttacgc cgccgcgcgg ggctactacc gccgaccgcg cgcattgcgt cgtatgcgtc
    18541 tcaaaacttc gttatgaaaa agggatagat gtactgctac aggcgtggcg acaggtgcag
    18601 gcgcagctat caccgccagg gagcgcgcgg ctggtgattg tcggcgacgg ccccctccgc
    18661 gccgagctag agcgcctggc tggggagctg gacatcgccg acagcgtcga attcgcgggc
    18721 ttgcagagcg atgtaccagc acaactctat cgaggcggcc tggccgtgct tccttcgcgt
    18781 tgggagggga tgcccaacgc cctgctggaa gcgatggcct gcgggctggc ctgcgtcgcg
    18841 acgcgcgtga gcggcagcga agatatcatc cagcacggca tcaacggcct gcttgtcgaa
    18901 cccggtgata acgaaggcat ggcacaggcg cttttaaccc tgctgcgcga ctccgccctg
    18961 gccggagtgt atgggcgggc cgcgcgtgaa accgtcgaac agcagtatgc gtttgaacgc
    19021 attatgaata cgtatgttga actatatcgc tctctggtgg gtgagacttc tgttaaggcc
    19081 gaagatacat cggtatctga aatgtctcac gtactatgac aactctgtag gcgccgattt
    19141 accgcgcgcc tacacagcat atgccaaacc ctccagaagc gagtgaaagg atagacgttt
    19201 atgtgtggca tcgcaggttt tgtcgatgcg gagatcggcc tgaaccaggc cgagcagttg
    19261 attgacggca tgtgccaggt catccggcac cggggacccg acgaccaggg aacctgggtg
    19321 ggtgatggcg cagcactggg gatgcgccgg ctctctatta tcgatgtgtc gggcggcaac
    19381 cagccaattt ttaatgaaga tcatagcatc ctgatcgtgt tcaatggaga aatttataac
    19441 tatctagaac tcagagatga acttcaggcg cgtggccatc atttcgccac caatagtgat
    19501 acggaagcca tcgttcatgc ctatgaagag tacggcgacg attgcgtcaa gcgcctgcgc
    19561 ggcatgttta cctttgccat ctgggacagg aagcgccacc gcttgctcgc ggcccgcgat
    19621 cgttttggca agaagccgtt gaattattac tgggatgggc agcgattgat ctttggctcc
    19681 gagatcaaat ccatcctcca ggctggtatt ccgcgcgaag tcaatcccat cgctctcgat
    19741 gagtacctgg tctatcgcta tgttccgacg ccaaacacgc tgttcaaaaa cgtcatgaag
    19801 ctgcccgctg cccatatctt gatctacgag catgggcagg tcagcaccag gcgctactgg
    19861 gagctgccct ttacgccgac ctgtcaggat gatgaagcga cggcgagcga gcgtatcctc
    19921 gaactgctca aggacgcggt gaaggtgcgc ctgatgagcg aggtgcccct gggcgcgttc
    19981 ttgagcgggg gcgtcgattc aagcgtggta gtaggcttga tgagccagat gatgtcgcag
    20041 cccgtcaaaa ccttctcgat cggcttcgag gaggattatt acagcgaatt gccgtacgct
    20101 cgccagatcg cgcaacactt cgataccgag catcacgagt tttttgtgcg gccagagttg
    20161 atatcggtgc ttccccagct cgtctgggcc tacgatgagc cgtttgccga cgcatccatg
    20221 ctgccaacct attacgtctc gaaactggcg cgtgaatatg tcacggtggc gctcaccggt
    20281 gacggcggcg atgaaatctt tggcggctat gtgcattacg agcgcgaact gatgacgagc
    20341 cgcatccctc tgctcatgcg cctcttgctg ggctatggca gcctgttgat gccgaatggg
    20401 atgcgcggca aaaaacgctt ccacaacatg ctctacgacc acgccaggcg caatgtctgt
    20461 tcaaccacgc tctttcccga aaattcgcgt cacgccatgt atagccgcga ctacttcgag
    20521 cgagtgtgcg atcacgatcc atttgaacgc ctcttgagtc agtatcgcaa cgtgccgaac
    20581 cttgatgtca cggccagtat gcaatatgtg gacgcgcgcg tgtacctgac cgatgatatc
    20641 ctggtcaagg ttgataaggc gagtatgttc aactcgctcg agacgcgcgc tcccttgctc
    20701 gaccagcatc tcgtcgaata tgtcgcgtcg ctgccttcgt cgctgcgcct gcaaaacggc
    20761 gtgttgaaat acttgctcaa gaaggtcgcc ctggatatgg tcccagctga gacggtgaac
    20821 cggcccaagc acggcttcag cgttcccatc aagcagtggt tccgcggcga cctgttcgcc
    20881 tatgcccgcg aattgctcga gtctccacgc gcgcagcaac gcggcatttt caacccgcag
    20941 ttcgttagca atatgctgaa aacgcacgcc agcaccaaac ttgtgaattt cagcgaggcg
    21001 atctgggcgc tggtctgcct ggaactgtgg ttccaggtct acatcgacga accgcccctg
    21061 cgcgtcgttg agaccccaca gatccaggtg gcgaaccatt agggggaggg atacatggac
    21121 gacccaatac taccgcgcga cgccaggaaa ccctaccggg tcttgatggt cacgggcatt
    21181 tacccgacgg ctgcccgtcc cgataaaggc acctacatca aaagccaggt cgaatcgctg
    21241 atcgccgccg gtctggaggt cgaggttatt catccgaagc ctgggcctgc tcccctgcgt
    21301 tacgccagcg cgtatcttca ggtcttcctc aaaacgctga ccagaaaatt cgatgtcgta
    21361 catgggcatt atggtatatg gtgcctgacc gcgcggttgc aatggacgac gcccgtcgtg
    21421 gcctccttcc tggggtcgga cttactgggc gtcgtaacag ccgacggcgg atttagctct
    21481 tttggagagc gagttgcgcg catcagtcgc tggctctgcc gccacgttga tgccgttatc
    21541 gtcaaatcgg aggagatgaa aaacgccgtt cagggaaagg gcagcatctt cattattccc
    21601 aacggcgtcg attttgatct gtttcgcccg cgcccgcgcg cgcaggtacg cgcagaactg
    21661 ggctgggacg ctgaccgttc gtatgtcctt tttggcaacg atcccaggcg gacggtcaag
    21721 aatttcccgc tggcccaggc cgccttcgag cgcttacaca cacgaggagt agacgcggag
    21781 ctggtggttg ccaatggcct gccgcatgag caggtcgccc actacatcaa tgccagtaac
    21841 gccgttctcc tgacctcctt taccgaggga tcgcccaaca tcgtcaaaga agccatggcc
    21901 tgcaacgtgc cggtcgtctc caccaatgtc ggcgatgtcg cccagcttat cagcgacact
    21961 gatggctgcg ccgtctgccc gggcgatccg gacgcgctgg cggatgcgct ggtagtggct
    22021 ttgcatcata ccgagccgac caccggcagg agcaacatcc agcacctgga caggtcggct
    22081 gtggcccccc aggtgatagc ggtgtacgag caggtcatca agaaa
//
LOCUS       DASZAK010000422         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_116501, whole genome shotgun sequence.
ACCESSION   DASZAK010000422 DASZAK010000000
VERSION     DASZAK010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116501"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2244)
                     /locus_tag="VGT44_07415"
     CDS             complement(<1..>2244)
                     /locus_tag="VGT44_07415"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV2580667.1"
                     /translation="EFVALLDRETVPTLLMRRLMEIVDEGEYEGQVKRLARIIGNLGA
                     DAVPLAAPLSRAAAGHSSRLRTAAINILGGTGDQEAVAPLDACLYDADSFIVNRAASA
                     LYRLGPALVLPALADELENVSQTTATLQVHIVALRILERFLNEEDAARRLKPSQRQQV
                     MAILMNILNQSYAAEVQEVAREMLVRQGQTAEKGAGGEMAVEALIQNLSSSDSQLART
                     AISALCQIGPAATPLLLQQLRQQPSEPVTARVVETLGKIRDARALSPLLRLLGEPSVA
                     VQQQVAQALLRYGDDSIPGLIYQILQGESELVATSAERILGELGESAVDPVTEALIPV
                     VAGRTHLLVRVLARLHSPLSALPLIALLESATHEAPVDTQLVLALIEGLGQFQDERVV
                     APLMEMLISANPLFYEGAINALSNLGPLACSELVAALDVGQETVITQRIERALLGMES
                     FPGECLLEAFISGSDAQVEHVMHVFMVSGAEAAQFVVNNLNHPNRQVQLYTRAAAELM
                     PGAIIVPALIDGIDHPDRNVRAVVAENLLKHPDEAIPLLVEMLQDNSLGSLSQRLLLE
                     FGPPILPLLIPALDSLSDLAYERARQLVIEVARQSPQTLREVVLLFSLGLPQRAHQAL
                     VSVLADELADISVPALLNGLQDAHLIAAVSETLGYMVNKRDARSPFILDELLAALRPN
                     THRQGAEIALVEIGAEAVPGVGNLITDHDPIVARTAQDILCQIGVPAFAFIWAAHSDV
                     ANRPRREA"
BASE COUNT          434 a          680 c          719 g          411 t
ORIGIN      
        1 ggcctcccgg cgcgggcggt tggcgacatc gctgtgggcg gcccagatga aagcaaaggc
       61 aggcacgcca atttgacaga ggatatcctg cgccgtgcgc gcgacgatgg ggtcatggtc
      121 ggttatcaag ttgcccacgc ctggcacggc ctccgcgccg atttcgacca gggcgatttc
      181 ggccccctgg cgatgggtgt tcggacgcaa ggcggcgagt aattcgtcga gaatgaaggg
      241 actgcgcgca tctcgcttgt ttaccatgta tcccaatgtc tcggagacgg cggcgatgag
      301 gtgcgcgtct tgcaagccat tcagcagcgc cggaacgctg atatcggcca gttcatccgc
      361 gagtacgctc accagcgctt gatgggcgcg ttgtggcagc ccaagcgaga aaagcaggac
      421 aacctcgcgc agcgtctgcg gcgattgacg cgccacttcg attacgagct gccgcgcgcg
      481 ctcatacgcc aggtcgctca acgagtccag ggcgggtatg agcaatggca gaataggcgg
      541 tccaaactcc agcaggaggc gctgggacag gctgcccagg ctgttgtctt gcagcatttc
      601 cacgaggagc ggtatggcct cgtcgggatg tttgagcagg ttttctgcca cgaccgcgcg
      661 cacattgcga tcgggatgat cgataccgtc aattaaagcc gggacaataa tcgcaccggg
      721 catcagttct gctgccgcgc gtgtgtagag ctgcacctgg cgattggggt gattaagatt
      781 gttaaccacg aattgcgccg cctccgctcc gctcaccata aagacatgca tgacgtgttc
      841 gacctgcgcg tcactgccag agatgaaggc ttcgagcagg cattcgccgg ggaaggactc
      901 catgccgagc aaggcgcgtt cgatccgctg cgttatgacg gtctcctgcc cgacatccag
      961 cgcggctacc agttcggagc aggccagtgg tcccaggttg ctcaaggcat tgatcgcgcc
     1021 ttcgtaaaag agcgggttgg cgctgatcag catctccatc aatggcgcaa caacgcgctc
     1081 gtcctggaat tggccaagcc cctcgatcag cgccaggacc agctgcgtat caacgggagc
     1141 ctcgtgagtg gctgattcga gcagggcaat caggggcaaa gcagagagcg gagagtgcag
     1201 ccgcgccagc acacgcacga gcaggtgtgt gcgaccggct acgacgggga tcagcgcctc
     1261 cgtcaccggg tcaacggctg actcgccaag ctcgccgaga atgcgttcgg cgctggtggc
     1321 gaccagttcg ctttcgccct gcaagatctg gtaaatcaaa ccgggaatgc tgtcatcgcc
     1381 gtagcgcagc agggcttgcg cgacctgctg ttgtaccgcg accgatggct cgcccagcag
     1441 gcgcagcagc ggggaaagcg cgcgagcatc gcggatcttc cccaatgttt cgacaacgcg
     1501 cgcggtgaca ggctccgatg gctgctggcg cagctgctgg agcagcagcg gcgtggcagc
     1561 cgggccgatt tggcataagg ctgagatggc agtgcgggcc agttgactat cgctggaaga
     1621 aagattctga atcagcgcct cgaccgccat ttcaccgccc gcgcccttct cagcagtctg
     1681 cccctggcgc acgagcatct cacgcgccac ttcctgcact tcagctgcgt aggattggtt
     1741 gagaatgttc atcaaaattg ccatgacctg ctggcgctgg gagggcttga ggcggcgagc
     1801 agcgtcttct tcattgagga agcgttcaag aatgcgcaga gctacgatat gaacttgcag
     1861 cgtagccgtc gtttgcgaga cattctccag ttcgtcggcc agagctggca ggacaagcgc
     1921 gggaccaagc cggtagagcg cgctggccgc cctattgacg atgaaactat cagcgtcgta
     1981 caggcaggca tccagcggcg caactgcttc ctggtcgccg gtgccgccca ggatattgat
     2041 ggccgccgtg cgcaggcgcg aactgtgccc cgcggcggcc ctgctcagcg gcgcggcgag
     2101 cggcacggcg tcagcgccca ggttcccgat gatgcgcgcc aggcgcttga cctgtccctc
     2161 gtactcgccc tcgtcaacta tctccatcag cctgcgcatc aacagcgtcg ggacggtttc
     2221 cctgtccagc agggctacaa actc
//
LOCUS       DASZAK010000423         1601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_542748, whole genome shotgun sequence.
ACCESSION   DASZAK010000423 DASZAK010000000
VERSION     DASZAK010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542748"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..420
                     /locus_tag="VGT44_07420"
     CDS             148..420
                     /locus_tag="VGT44_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580668.1"
                     /translation="MGLISNVLNQCRKPTGWFGRWLVWKMNRHHSKATGWGLQHLARE
                     RRDTILDVGWGKADSAHAPGIATQGRVYGIDFSEESMSVSCRRNKQ"
     gene            427..732
                     /locus_tag="VGT44_07425"
     CDS             427..732
                     /locus_tag="VGT44_07425"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2580669.1"
                     /translation="MGGVEIQHSSVSCLAFSDRMFDLVTAVNTHYWWPDLVADRHEVL
                     RIQKLGGKLIIIAEAYKGRKYKKVSQKLVGLLKTMNDAHLSVTEPRELFSRAGYTAV"
     gene            953..>1601
                     /locus_tag="VGT44_07430"
     CDS             953..>1601
                     /locus_tag="VGT44_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEV2580670.1"
                     /translation="MDALDVETLALDDLVLPLHSQKAELSTVGGKGANLSQLARAGFP
                     VPAGFLVSTAAYRTFVQDNDLQPQIVALARSGAGTDFEAVSAAIRQLFEGASIPSQVT
                     SAIQQAYAALCTEGQAPIPLAVRSSATAEDLPGASFAGQQETYLNVRSKQDVLQAVKR
                     CWSSLWTPRALAYRARQGIDPATVSLAVVVQTLVPADVAGVLFTANPLTGARDEIV"
BASE COUNT          343 a          454 c          499 g          305 t
ORIGIN      
        1 aaagctgacg ctcatagaac gctccataga acgctttctt cacgctgcct atgctagact
       61 agcggtgaaa agaggatgca gaccgtgttg tgcggacgat catagcacat ccgctgacac
      121 aggtatcttg acaagaggag gaccatcatg ggcctgatca gcaacgtgct caatcaatgc
      181 cgaaaaccga ccgggtggtt cggcagatgg ctggtgtgga aaatgaaccg tcatcattcc
      241 aaagcgaccg gctggggatt gcagcatctc gcgagagagc ggcgcgacac gattctcgat
      301 gtgggctggg ggaaggcgga ctctgcacac gctcccggca ttgctacgca gggcagagtt
      361 tacggcatcg atttctccga ggagagcatg agcgtctcct gtcgaagaaa caagcagtga
      421 aacaagatgg gcggcgtcga aatccagcac agttcggtgt cttgcttggc gttctccgac
      481 cgcatgtttg atctcgtcac ggcggtgaac acgcactact ggtggcctga cttggtcgca
      541 gatagacacg aggtcttacg catccagaaa ctgggcggaa agctgatcat catcgccgag
      601 gcctacaaag gcaggaaata caagaaagtg tctcaaaagc tcgtaggatt actgaagacg
      661 atgaatgatg cgcacctgag tgttactgag ccccgcgagc tcttctcaag ggccggttat
      721 acagctgtgt gaggacgacg acaaagggtg ggctgtgggg tgcattaaaa aagttgacga
      781 gagtcaagcc accagaagag actgctgctc atagcacgga tcgtctgtgc catgaggcac
      841 agtggtgcct acggccagaa ccgcatcgag ccgctctggt gggaagccgc atgcttgcct
      901 tccggcacca ggcatgcgtg gcggggtgcc tgagacagaa aggaggggca tcatggatgc
      961 tctcgatgtg gagacgctcg ctctggatga tctcgtgctt ccgctccatt cgcagaaagc
     1021 cgagctctct accgtcggcg ggaaaggggc caacctgagc caactggcgc gagccgggtt
     1081 cccggtcccc gccgggttcc tggtgagcac cgccgcctac cggaccttcg tccaggacaa
     1141 cgatctccag ccgcagatcg tggcactggc tcgcagcggt gctggaacag actttgaagc
     1201 cgtttcagcg gccattcgcc agctcttcga gggcgctagc atcccctcgc aggtgacctc
     1261 tgcgatccag caggcgtatg cggcactctg cacggagggg caggcaccga tcccgctggc
     1321 ggttcgctct tctgctacgg ccgaagatct gcctggtgcc agtttcgctg gccagcagga
     1381 gacctacttg aatgtccgta gtaaacagga cgtgttgcag gcagtgaagc ggtgctggtc
     1441 cagcttgtgg actccccgcg ccctggccta tcgcgcccgc cagggcatcg atccggccac
     1501 cgtcagcctc gcggtcgtgg tgcagacact ggtccccgcc gacgtggctg gtgtgctgtt
     1561 taccgccaac cccctcacgg gagcgcgaga cgagatcgtg a
//
LOCUS       DASZAK010000424         3931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_82171, whole genome shotgun sequence.
ACCESSION   DASZAK010000424 DASZAK010000000
VERSION     DASZAK010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82171"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..620)
                     /locus_tag="VGT44_07435"
     CDS             complement(123..620)
                     /locus_tag="VGT44_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580671.1"
                     /translation="MSSPATTNDTVFVGPGLSPFKRVVTILGVIAMTALVLWIALHEG
                     LPAIGSTILALLFILGFIIYLRIIAPVPFTIALEQEALVKRGKQGEAIEVKWEDLTRV
                     KEEFFPNGKRISITAYRKPATPEQKTKAWAVYRDDVTDIDGLAEALKQRIPPTCQWSS
                     ETVHE"
     gene            827..1825
                     /locus_tag="VGT44_07440"
     CDS             827..1825
                     /locus_tag="VGT44_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV2580672.1"
                     /translation="MTTGKLLATQGYVVGIEISGSGLRQSVTLADLEGKVLHQVRRPL
                     DYVPDTTTVLKLIDEMLDETLTTERVCDGRVLRVGVAVGGLVDASRGIVRTLHHAHGW
                     NDFPLQDYLAKRLDRPCIIDNNANAAALAEVYFGVGQRERVVCYIGLGRGIGGGLVVD
                     GKIYHGVSSTAGEIGHMAVKENGPKCSCGSFGHLEAIASAQAIVRTMIGLSVEHPETE
                     AAIRRITGNRAERMTAEQVFLLAAEGDAIAQGVTQEVYTYLGIALANIVHMINPGMII
                     LGGQVAQVGDLLIQPLQARLRDLCLSAATQSLRVEQGALGSEANSVGAITLALQDI"
     gene            1841..2995
                     /locus_tag="VGT44_07445"
     CDS             1841..2995
                     /locus_tag="VGT44_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2580673.1"
                     /translation="MDTGRLIQERYRLQQLIKQGQTAAVYYGWDEVLSRAVVVKSVPA
                     PYMADYRAALKLTSHFSHPNIVSMYDLVIETDLLHIVQEYIEGDDFVALMQKQMTPFA
                     VIDLGSQLCQALLYAGSPARRIAHGDLTPSAVMRDRNGFVRVNNFALPSDVGYFQRWS
                     VMGGDGIVVSDTELPWGTWSDDRQADDTRAVGLLLYQLLASRIPGTSVVEPRPDGRLS
                     FQRSVPPEVCEMVARAVARQHPEAIRTPEALFVGLKTLAETLEPVLPTAAPITSAQFE
                     PVSARQYSPAGSKLATALPLDRTGAEAVPYRPVSVPNAGLSIDEAAPSSPTVADQSLK
                     LAAARQAAYSTRPAPSQERSSTLLILLICLIVFAALFIIGFVLGQTIIPH"
     gene            complement(2989..>3931)
                     /locus_tag="VGT44_07450"
     CDS             complement(2989..>3931)
                     /locus_tag="VGT44_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEV2580674.1"
                     /translation="NLIMPIAIIVHGGAGDIAPERRADADAGCKAAALVGWRVLRDGG
                     AALDAVEAAVRALEDNPEFNAGTGACLNIDGNIQLDAGIMDGVHLQVGAIAGIERIKN
                     PITLARRVLESPHVLLVGSGAVEFALEQGISQCALNDLITERQYAAWQEEIARREAEE
                     PRIHRREVRSVQARPGEHGTVGAVAMDQSGALAAATSTGGIHNKYPGRVGDSPLIGCG
                     FYADEYAAVSCTGHGEDFVRLMIAKRAADAVASGRSAREAALSAIAFLGARATGTGGL
                     IVVDHHGSIGFAWNSNHMSYAYLTEDVEHPVPTHNLQ"
BASE COUNT          784 a         1120 c         1200 g          827 t
ORIGIN      
        1 gataatgtta gtggcagcgg cgatggcctt gataagcgcg ctcagttcat ttttgttgat
       61 cgaggggaaa cggcctgcga tagatgcaaa ggaagaaaat taactatatg cccggtaagt
      121 gattattcgt ggacggtctc gcttgaccac tggcaggtgg gggggatgcg ctgcttcaag
      181 gcttcggcca acccatcgat gtcagtaaca tcgtcgcggt agactgccca tgcttttgtc
      241 ttttgctcag gggtcgcggg tttgcgatag gcggtgatgc tgatgcgttt gccattgggg
      301 aaaaattcct ctttgactcg ggtcagatct tcccatttaa cttcaatcgc ttcgccctgc
      361 ttgccgcgtt tcacgagcgc ctcttgctcc agagcgatag tgaatggtac cggcgcgatg
      421 atgcgcaggt agatgatgaa gccgagtata aataagagcg cgaggatggt actgccgatg
      481 gccgggagtc cttcatgcag cgcgatccac agcaccaacg cggtcatagc gatgacgccg
      541 agtatggtga cgacgcgctt gaaggggctt agtcctggcc cgacgaatac cgtgtcgttc
      601 gtggtggccg gtgatgacat aacgttatgc tacctcctac tcgatattgt aacaacgtga
      661 cgggtacaac gcaatatgct atactgaatg ctctgttacc gtcgtttgca tcgtaccgtc
      721 ctataccgta acgcccgtga tactagcatg ggaataagcg tattgtccat agaccgatag
      781 atcagttgat cggcaaaacc ggcgagcaag agaaggagat gagtcgatga caaccggcaa
      841 actgcttgca acccagggat atgtcgtagg aatcgagatc agtggcagcg gattgcgcca
      901 atcggtgacg ttagcggacc tggaaggcaa agttttgcac caggtgcgcc gcccattgga
      961 ttatgtaccg gataccacaa ccgtcctcaa gctgatcgac gagatgctcg acgagacgct
     1021 gacgacggag cgggtgtgcg atgggcgcgt gctgcgcgtg ggggtagcgg tcggcgggtt
     1081 ggtggatgct tcgcgcggca ttgtgcgcac attgcatcac gcgcatggct ggaacgactt
     1141 cccactgcag gattacctgg cgaagcgact tgatagacct tgtataatag acaacaatgc
     1201 gaacgcggcg gccctggcag aagtctattt cggcgtaggg cagcgcgagc gggttgtatg
     1261 ctacatcggc ctgggacgcg gtatcggcgg cgggctggtg gtggatggca agatttatca
     1321 tggtgtctca tcgaccgccg gggaaatcgg gcatatggcg gtcaaggaga acgggccgaa
     1381 atgctcgtgc ggcagctttg gacacctgga agcgattgcc tcggctcagg ctattgtacg
     1441 cacaatgatc ggtctttcgg tggagcatcc tgagacggag gcggccattc gccgcatcac
     1501 aggaaaccgg gctgaacgca tgacggctga acaggtcttt ctgctggcgg cagagggcga
     1561 cgcgatcgcg cagggcgtca cgcaagaggt ctatacctat ctgggcatcg ccctggcgaa
     1621 tattgtacat atgattaatc caggcatgat tatacttggc gggcaggttg cccaggttgg
     1681 ggatttattg atccagccct tgcaggctcg cctgcgcgac ctgtgcctga gcgcggccac
     1741 ccaatcgctg cgcgtcgaac agggcgcgct tggctcagaa gcgaacagcg taggagcaat
     1801 tacccttgct ttacaggata tataaggacg agaaatactg gtggatactg gtcggctcat
     1861 tcaagagcgc tatcgattac aacaactgat caaacaggga caaacggcgg cagtgtacta
     1921 cggttgggac gaagtgttgt cgcgcgccgt cgtggtgaaa agcgtccccg cgccctatat
     1981 ggcggactat cgcgctgctc tcaagctgac ctcgcacttc tcccacccca atatcgtcag
     2041 tatgtacgac ctggtgattg aaactgatct gctgcatatc gtgcaagaat atattgaagg
     2101 cgacgacttc gtcgcgctga tgcagaagca gatgacccct ttcgctgtga tcgacctggg
     2161 cagccagctc tgccaggcgc tgctctacgc gggcagccct gcgcggcgta ttgcgcacgg
     2221 cgacctcacg ccttccgccg tgatgcgcga tcgcaacggt ttcgtgcgag tcaacaactt
     2281 tgccttgccg agcgatgtgg gctactttca gcgttggagc gtgatggggg gcgatggcat
     2341 tgtcgtgtcc gatacggaac tgccctgggg aacatggagt gatgatcgcc aagctgacga
     2401 cacgcgcgcc gttggtctac tgctttatca gttgcttgcc agccggatac ccggaacgag
     2461 cgttgtcgaa ccgcgtcccg atggccgact gagtttccag cgcagcgtgc cgccggaggt
     2521 gtgcgagatg gtggcgcgag ctgtagcgcg ccagcacccg gaagccatca ggacgccaga
     2581 agcgttgttt gtcgggttga agaccctggc agaaacgttg gaacccgttt tgccaactgc
     2641 tgcgccaatt accagcgcgc aattcgaacc ggtttctgcg cggcagtact ccccggcagg
     2701 gagcaagctg gctaccgccc ttccactcga ccggaccggc gcggaggccg ttccttatcg
     2761 ccctgtctcc gtcccaaacg cagggctatc tatcgacgaa gcagcgcctt cctcgcccac
     2821 cgttgccgat cagtcgctca aactcgccgc agcgcgccag gcagcctatt ctacccggcc
     2881 cgcgcccagc caggagcgtt cctcaacgct gttgatcctg ctgatctgcc tgatcgtgtt
     2941 tgctgctctt tttattatcg gcttcgtgct agggcagacg attattcctc attgaagatt
     3001 atgagtaggg acagggtgtt caacgtcctc agtcagatag gcgtaggaca tatggttgct
     3061 attccacgcg aagccgatgc tgccatgatg atccacgacg atcaggccgc cggtgccggt
     3121 tgcgcgcgcg ccgagaaagg cgatagcaga aagagcggcc tcgcgcgcgc tgcgaccgct
     3181 ggcgacggca tcagcggcac gtttggcaat catcaggcgc acgaagtctt cgccatgtcc
     3241 ggtgcaagag actgcagcgt actcatcggc atagaagcca cacccgatca atggcgaatc
     3301 gcccacgcgc cctggatatt tattatggat gccgcccgtc gaggtggccg ccgccagcgc
     3361 gccgctctga tccatggcga ccgcgccgac ggtgccatgc tcgcctggac gcgcctgcac
     3421 cgagcggact tcgcggcggt ggatacgcgg ctcctcggcc tcgcgccgcg ctatctcctc
     3481 ttgccaggcg gcgtattgcc gctcggtgat caagtcattc aacgcgcact ggctgatgcc
     3541 ctgctccagc gcgaactcta ccgcgccgct gccgacgagc agcacgtgcg ggctttccag
     3601 tacgcggcgc gctagcgtga tgggattctt gatgcgctcg ataccggcaa tggcgccgac
     3661 ttgcaggtgg acgccatcca tgatgcctgc atccagttga atattgccgt cgatgttcag
     3721 acacgcgccc gttcccgcgt tgaactccgg attgtcttcc agcgcgcgca cagcggcctc
     3781 gaccgcgtcc agcgccgcgc cgccatcgcg caagacgcgc caccctacca gtgccgccgc
     3841 cttacagcct gcgtcggcat ccgctcttcg ctctggcgcg atgtcgccag cgccgccgtg
     3901 tacaatgatt gcgatgggca taatcagatt c
//
LOCUS       DASZAK010000425         6408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_472206, whole genome shotgun sequence.
ACCESSION   DASZAK010000425 DASZAK010000000
VERSION     DASZAK010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6408
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472206"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..2502
                     /locus_tag="VGT44_07455"
     CDS             64..2502
                     /locus_tag="VGT44_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEV2580675.1"
                     /translation="MMLRRGRSSALSLPARFNLALLARLATIVVLTGLIVLIWSLSHL
                     YSGQKSLLLTAQQIASDQQSPAFGVQFPAQSFANTFPVVFWWLCIALLGALAFPLAFT
                     ALRGLADRGYIFSKTLGILLLAYLAWLLASAQLVAFSHISMFIVTGIMLAASAVALFL
                     QRHTMLAYLRQHWRLLLIEEGLFTLAFLLFVLIRSFDPDLWNLYLGGEKPMELAFLNA
                     VLRSPYMPPLDPWYAGGYINYYYYGFIVIGALVKLTGMAPTTAFNLAIPTLFALTFSG
                     AVAIVYSVTRRLPFALLGGYFAALIGNFDGFTQLREQLQAALAHLPVPQFSYWRSSRI
                     IPFTINEFPFWSFLFADLHPHVINMPVVLFMLGIIAALFVEENVALYMLAAFVLGTLA
                     CINPWDMPVYALLLAVTLLLRRFFATSGQPMRQRLGALSITLVSTVALLLASFLLYWP
                     FYASYQQLYVNGLGLVTSGTSLGDYLTINNLWIFLALSFFLLELYRWWTRRLTYRASV
                     VDFLMLCGVVLIFAALLGLKVLLIALIALGVFLLVVQRETKTRYIILLLLGGLCISLG
                     LELVYVRDFLDGSVNERMNSVFKFSIQAWFCFAIGGALAAWRVWKGLGGAMRRAWLAG
                     FALLLVANSVFLPIGTLSRLNDHQAWALDQPPPQSATYLPTLDGVAFMRAWYPGDAQA
                     IAWINEHIAGSPVILEAASPYSFAWFNRVSVYTGLPDVLGWPDHVGEQRNSEQVLNRL
                     VDVGIIYTTQNTTLALLLLHSYHVRYIYVGALERQIYTQQTTSGLDKFDAMVGASLRV
                     IYRSGGVTIYEVIV"
     gene            2686..5352
                     /locus_tag="VGT44_07460"
     CDS             2686..5352
                     /locus_tag="VGT44_07460"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HEV2580676.1"
                     /translation="MGELFQMWALVEVLGFICLPLTLTIFKNLPDRGWAFAKIVGMAL
                     LAFCVWLPLMTLQFLPFSQMFVFGVLLIILAMNVIGYPRVWKTLVELFREHRTYIITC
                     ELLYLGMIFLLGWVRSYNPNIQSYEMFMDEGFLASILRSQHFPPQDMWLSGYIINYYY
                     YAHYTIAVLAKLIGQPASIAFNTGICTFFGLTGVNIFGVTSNIVAWASKVSNRRQRQS
                     ARPARPARTGKTLARAMPYAIVSVLMCVILGNLASTQAWWQAHDGGLPYFYWFNVSRV
                     VDKTINEFPAFSFLLSCFHAHVLTLAFTILAIGLIFNLFLERGDGDDNDAQGLHIFGR
                     GWRLLLNLGFTAIVMGGLFTMNGWDFPTYLGTAIVCIVLQQWMAYHSRFSFDFVLDVF
                     TACASLTALSFFLYAPFYLNFISPSQGVGIVDAANRSTVASEMLIYGTFMFLFVTLLF
                     VNLLRDRVSPSDIVQALRNGTGPMPIMPFLASLSKPANGASGEQGIHGTGLLTRTGGV
                     EEEMVGGELSVTGEHASAWARQQTATVPQTLPSASEEDTARDDVVIEPISPLPPPAQE
                     PARRLSWISFRLVSQVFIMVAALLIYLLVKNSLWLVIAFVITALGAEMVLYHAQDRPR
                     AFVLLLGTVAFGLVAMTEVVYLRDVFASTYPRMNTVFKFYFQAWALLSITSGAGLYFI
                     FESFRPTFDLDGLQLVIRRSGQVIWTAMFLLLLLAGAVYPIFGSYQRTNEYQQRTNSL
                     DGMAYLQQYAPGDYYAIQWLNAHVQGDPVIVEAYGKQGGDYSDYGRVSAFTGLPTVIG
                     WAGHEYQWRVNWLNNDYNAADFYRRGSDVNLIYTSTDPQTVLATLERYRVKYLYVGNL
                     EIATYSSSNLGRFSKFMPVVYSYDGVTIYQVP"
     gene            5386..>6408
                     /locus_tag="VGT44_07465"
     CDS             5386..>6408
                     /locus_tag="VGT44_07465"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HEV2580677.1"
                     /translation="MKSFDTRAREHENEGNEFSNEIEVTLPESARAQVTPDEQEGQVD
                     QPLLPPEAEDNIGSLRVLGAPYDPARDAPPERRRLRLRPPTREQIIRWAPFWGLILLG
                     AILRFWGLGDKPLHHDESLHAYYSLQLLGNLEHWSWCIQPPPQNPGYACYTYNPLLHG
                     PFQFHAIALVYWISQRLGAPDNGVNTTTVRITAATLGTLMVGLPYFLRDHLGKWGAWI
                     ASFLLAVSPTLVYFSRFAREDIYFAFFTFVMIVGVARYVRDRKMRWLVVAAAGFSFAY
                     ATKESIFLMMMTFGCFLGALAVWELGLRVSLRSRLGDENAPNPFIPQTFAPVFTLIYL
                     MIAGIAG"
BASE COUNT         1099 a         1934 c         1804 g         1571 t
ORIGIN      
        1 atagcgccag ttgttgagtc tatcgtcccg caagagcgag gaagaaagta gccgagccga
       61 actatgatgc tgcgccgggg ccggtcaagc gcactttctt tgcccgcgcg cttcaacctc
      121 gcactgctcg ccaggcttgc cacgatcgtt gttctcaccg gacttatcgt actgatctgg
      181 agcttgagcc acctgtacag cggtcagaaa tcactgctac tgactgcgca gcaaatcgcc
      241 agcgaccagc aatcgcctgc ctttggcgta cagtttccag cgcagagttt tgcgaacacc
      301 ttcccggtcg tcttctggtg gctctgcatc gccctcctgg gcgcgctggc ctttcccctg
      361 gcgttcacgg ccctgcgcgg gctggccgac cggggttata tcttcagcaa gacgctcggc
      421 atcctcctgc tggcctatct tgcctggtta ctggccagcg cgcagcttgt cgctttcagt
      481 catatatcga tgtttatcgt tacaggcatt atgctggctg ccagcgccgt agcgcttttc
      541 ctgcagcgac atacgatgct ggcctatctc cggcagcatt ggcgtctgct gctgatcgaa
      601 gagggattat tcacgctggc cttcttgctc tttgtcctca tccgctcgtt tgaccccgac
      661 ctctggaatc tttacctggg cggtgaaaag ccgatggagc tggctttcct caatgctgtg
      721 ctgcgcagcc cctacatgcc gccgctcgat ccctggtatg ccggtggcta catcaactac
      781 tattactacg gctttatcgt catcggcgcg ctggtcaaat tgaccggcat ggccccgaca
      841 acggccttta atctcgccat cccaacgctc ttcgctctga ccttcagcgg cgctgtcgct
      901 atcgtttaca gcgtgactcg tcgcctgcct ttcgccctgt tgggtggcta cttcgccgcg
      961 ctgatcggta acttcgacgg ctttacccag ctcagggagc agctccaggc ggcgctggcg
     1021 catctgcccg tcccgcaatt cagctactgg cgcagcagcc gtatcatccc attcaccatc
     1081 aacgagttcc cattctggag ctttctcttc gccgacctgc atccgcatgt catcaatatg
     1141 ccggtcgttc tcttcatgct gggcatcatc gcggctttat ttgttgagga aaatgtcgcc
     1201 ttgtatatgc tggccgcctt cgtcttgggc acgcttgcct gcatcaaccc ctgggatatg
     1261 cccgtctatg ccctgctgct ggcggttacg ctgctgctgc gccgcttctt cgcgacgagc
     1321 ggccagccca tgcggcaacg tctcggcgcg ctgtccatca cgcttgtcag cacagtcgcc
     1381 ctgttgctgg catccttcct gctctactgg ccgttctatg ccagctacca gcagttgtac
     1441 gtcaacggcc tgggtctcgt cacatcggga accagcctgg gcgattacct gaccatcaac
     1501 aatttgtgga tcttcctggc tctcagcttc ttcctgctcg aactctaccg ctggtggacg
     1561 cggcggctga cctatcgtgc atcggtggtt gactttctta tgctctgcgg cgttgtgctg
     1621 atctttgctg ccttgctcgg cctcaaggtg ctgctgatcg cgctgattgc gctgggcgtg
     1681 tttctgctgg tcgtgcagcg ggagacaaag acgcgctaca tcatcctctt gctgcttggc
     1741 ggcctctgca tcagcctggg gttggagcta gtttacgtgc gcgattttct ggatggcagt
     1801 gtgaatgagc gcatgaacag cgtgttcaag ttctcgatcc aggcgtggtt ttgctttgct
     1861 atcggcggcg cgctggcggc gtggcgtgta tggaaagggc tgggtggagc gatgcggcgc
     1921 gcctggctgg ccggttttgc cctgctgctg gtggcgaact cggtctttct gccgatcggc
     1981 acgctctctc gactgaacga tcaccaggcc tgggcgctgg accagccgcc gccacagagc
     2041 gcgacctacc tgcccacgct ggacggggtg gccttcatgc gcgcctggta tccgggcgat
     2101 gcccaggcca tcgcctggat caacgagcat atcgccggtt cgccggtcat cctcgaggct
     2161 gcttcgcctt actcgttcgc ctggtttaat cgcgtctcgg tctataccgg cctgccggat
     2221 gtgctgggct ggcccgacca cgtcggcgag caacgcaata gcgagcaggt cttgaatcgc
     2281 ctggtggatg ttggtattat ctacacgaca cagaacacga ccctggccct gctgctgctg
     2341 cactcgtatc atgtgcgcta catctatgtc ggcgcgctgg aacgccagat atatacccag
     2401 caaacgacga gcggcctgga caaattcgat gcgatggtcg gcgcttcgct gcgcgttatt
     2461 tatcgctccg gtggcgttac tatctatgag gtcattgtat aagacaaatg gcatagggac
     2521 ggaacgtatg cgataataca aacgtcataa tgttaagtag tcatacgtaa aattgtatgt
     2581 cgggctgcgt gttttttagt gtagaaatag gctgctatcg ccggagtaca gtagggtgcc
     2641 agggggccct gtacagcgac ttcctgaata gagtagagca aatcaatggg tgaactgttc
     2701 cagatgtggg cgctggtcga agtgctggga tttatctgtc taccgctcac gttgactatt
     2761 ttcaaaaatt tgcctgatcg cggctgggcg tttgcaaaaa ttgtcgggat ggcgctgctg
     2821 gctttttgcg tctggctgcc gcttatgacc ttgcaatttc tcccattcag ccagatgttt
     2881 gtctttggcg tactgcttat cattctggcg atgaacgtca tcggctatcc gcgcgtgtgg
     2941 aaaacgctcg tcgagctgtt tcgcgaacat cgcacgtaca tcatcacctg cgaactgctc
     3001 tacctcggca tgatcttctt gctgggttgg gttcgctcgt ataaccccaa catccaatcg
     3061 tacgagatgt tcatggacga gggcttcctg gcctcgatct tgcgcagcca gcatttccca
     3121 ccccaggata tgtggctctc cggctacatc atcaactact actactacgc gcactacacc
     3181 atcgcggtgc tggcgaagct aatcggccag cccgcctcga tcgccttcaa tacgggcatc
     3241 tgtaccttct ttggtctgac gggcgtcaac attttcggcg tcaccagcaa catcgtggcg
     3301 tgggccagta aggtctccaa tcgccgccag cgccaatctg ctcgtcccgc gcgtcctgcg
     3361 cgcaccggca agacgctcgc cagggccatg ccttatgcga ttgtctccgt gttgatgtgc
     3421 gtgattttgg gcaacctggc ctctacgcaa gcctggtggc aggcgcacga tggaggtctg
     3481 ccctatttct attggttcaa tgtcagtcgc gtagtagata aaactattaa cgagtttccg
     3541 gctttcagct tcctgctctc ctgcttccac gcgcatgtcc tgacgctggc cttcaccatc
     3601 ctggcgattg ggctgatctt caacctcttc ctggagcgcg gtgatggaga cgacaatgac
     3661 gcccaggggt tgcatatctt cgggcgcggc tggcggctct tgctcaacct gggcttcaca
     3721 gctatcgtga tgggcggcct cttcaccatg aacggctggg acttcccaac ctacctggga
     3781 acggccatcg tctgcatcgt cttgcagcag tggatggcct accactctcg cttcagtttc
     3841 gattttgtgc tggatgtctt caccgcctgc gcctccctga ccgcgctctc gttcttcctg
     3901 tacgctccgt tctacctcaa tttcatctcg ccatcgcagg gagtcggcat tgttgatgcc
     3961 gctaatcgtt cgaccgttgc gtccgagatg ctcatctacg gcaccttcat gttcttattc
     4021 gtcacgctgc tcttcgtcaa cctgctgcgc gaccgcgttt cgccgtctga catagtccag
     4081 gcgctgcgca atggcaccgg tccaatgcct attatgccat tcctggcgtc gctctcgaag
     4141 cccgcgaacg gggcaagcgg tgagcagggg atacatggta ctggcctctt aacccgcacg
     4201 ggtggagtcg aggaggaaat ggtcggcggc gaattgagcg tcacgggcga gcatgcatct
     4261 gcctgggcgc gccagcaaac cgccaccgtc ccacaaacgt tgccctccgc cagcgaagaa
     4321 gataccgcgc gcgacgatgt ggtcattgaa ccaatctcgc cgctgccccc gccagcgcag
     4381 gaaccggcca ggcgactatc ctggatcagc ttccgcctgg tctcccaggt gttcatcatg
     4441 gtagccgctt tgctgatcta cctgctggtc aagaatagcc tgtggctggt gatagccttc
     4501 gtgattaccg cgctgggggc cgaaatggtg ctctatcacg cacaggaccg cccgcgcgcc
     4561 tttgtgctgc tgctgggcac ggttgctttc gggctggtag cgatgacgga ggtcgtctac
     4621 ctgcgcgatg tgtttgcttc cacctaccca cgcatgaata ccgtcttcaa gttctacttc
     4681 caggcgtggg cgctgctctc gattacctcc ggcgcgggcc tctacttcat tttcgagagc
     4741 ttccggccta cgttcgatct ggatgggctg caacttgtca ttcgccgctc cggccaggtc
     4801 atctggacgg ccatgttcct gctgctgctg ctcgcggggg ctgtctaccc catctttggt
     4861 tcgtaccagc gcaccaacga gtatcagcag cgcacaaata gcctggacgg catggcctat
     4921 ttacagcagt atgcccctgg cgattactat gccatccagt ggctgaacgc gcacgtgcag
     4981 ggcgatccgg tgatcgtcga ggcgtatggc aagcagggcg gtgattatag cgattatgga
     5041 cgtgtctccg ccttcaccgg actgccgacc gtcatcggtt gggccggcca cgaataccag
     5101 tggcgcgtta actggctcaa caacgattat aacgcggctg atttctaccg caggggatcg
     5161 gatgtcaact tgatttatac ttcgaccgat ccgcagaccg tgctggcgac cctggagcgt
     5221 taccgggtga agtatcttta cgtagggaat cttgaaattg caacctatag cagctcaaat
     5281 ctgggccgtt ttagtaagtt tatgcccgtc gtctattcct atgatggtgt gaccatctat
     5341 caagtcccat aatcagggag gcgaaagcag gaggagtgtg atccgttgaa gagctttgac
     5401 acgagagcgc gcgagcatga gaatgagggg aatgaattct cgaatgaaat cgaggtgaca
     5461 ttgcctgaga gcgcacgcgc gcaagtaacg cccgacgagc aggaggggca ggttgatcaa
     5521 cccttgctgc cgcccgaagc ggaagacaac atcgggtcgc tgcgcgtcct gggcgctccg
     5581 tatgatccag cgcgtgacgc gccacctgag cgcaggcgct tgcgcttgcg cccgccaacg
     5641 cgcgagcaga tcatacgctg ggcgcctttc tggggcctga tcctgcttgg cgcgatcctg
     5701 cgcttctggg gcctgggcga taagccactg catcatgacg aaagccttca cgcctactac
     5761 tcgctgcagc tgctcggtaa tcttgaacac tggtcgtggt gcattcagcc acccccgcag
     5821 aatcccggct atgcctgcta cacctacaac ccgctgctgc acggcccgtt ccagttccat
     5881 gccatcgcgc tggtttattg gatatcccag cgcctgggcg cgcccgataa cggggtcaat
     5941 acgacgacgg tacgtattac tgccgccacg ctgggtacgc tgatggtcgg tctgccctac
     6001 ttcctgcgcg accacctggg caaatggggc gcgtggatcg ccagcttcct gctggcagtg
     6061 tcgccgacgc tggtctactt ctctcgcttc gcacgcgagg atatctactt tgccttcttc
     6121 accttcgtga tgatcgtcgg cgtggcgcgc tatgtgcgcg atcgcaagat gcgctggttg
     6181 gtagtcgccg ccgccggttt ctcatttgct tatgccacta aagagtcgat tttcctgatg
     6241 atgatgacct tcggctgctt cctgggcgcg ctggccgtct gggagcttgg cttgagggtt
     6301 tcgctgcgct cgcgcctggg cgacgaaaac gcgccgaatc cgttcatacc gcagacgttc
     6361 gcgccggtgt tcacgctgat ctacctgatg attgccggga tcgcgggc
//
LOCUS       DASZAK010000426         1703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_223728, whole genome shotgun sequence.
ACCESSION   DASZAK010000426 DASZAK010000000
VERSION     DASZAK010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223728"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VGT44_07470"
     CDS             <1..336
                     /locus_tag="VGT44_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580678.1"
                     /translation="RQVGEVEYAYIQSLKTFQQDWSYRNEEAGLESSVDRLRAWFETL
                     DEEMKATVSAFSDEDLNKSIDRGGGFTMPVDLQLDVYLQALLIFLGKATIYLKAMNKP
                     LPQPFQEYI"
     gene            complement(385..1650)
                     /locus_tag="VGT44_07475"
     CDS             complement(385..1650)
                     /locus_tag="VGT44_07475"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580679.1"
                     /translation="MSQAQYPPDSTGQGEQNRAPALPAKPVPLWRNRDYMLLWSGQIV
                     SSTGTRASQLALPLLVLALTHSPAQAGLVGAIRGIPYALFILPAGALVDRWNRKLVMI
                     LCDTGRALALGSIPLALLLGHMTIALFYIVSLVEGTLFTFFGLAETACLPHVVAKEQL
                     AGAAAQNSVIDSVSGMVGPSIGGILYGIARAIPFLTDAITYAASVLSIFFIKKDFQEE
                     RDIKPLRLWAEIGEGLSWLWRAPLIRFIALLTGGLILPVTGYSLILIVIAQGQHASSF
                     TIGLILASGGIGSIVGAMLVAPLQRRFRFGQLMIGSTWLWGLTWLLFAVAPNPFVLGV
                     AVAASFIVVPIYMSVQFGYRLSVIPDQLQGRVNSVFRLIAFGSEPVGMAITGFLLQAI
                     GPIPAVLVLFVPQGILAMVATFSKHFKGK"
BASE COUNT          404 a          469 c          511 g          319 t
ORIGIN      
        1 cgccaggtgg gtgaagtcga atatgcatat atccagtcgt tgaaaacctt tcagcaagac
       61 tggtcatatc gcaacgagga agcgggtttg gagagcagtg ttgacagact ccgagcgtgg
      121 tttgaaacgc tggatgaaga gatgaaagcg acggtttctg ctttctcgga tgaggatttg
      181 aacaagtcaa ttgatcgagg aggtggcttt acaatgcccg ttgacctcca actcgatgtc
      241 tatctccagg cgctgctgat cttcctcgga aaggccacaa tctacctgaa ggcgatgaac
      301 aaaccgttgc ctcaaccatt tcaggaatat atctgatagg gaattcgtgt atattcatca
      361 tcagcctgca tgcatcacct atcattattt acccttgaaa tgtttgctga acgtcgctac
      421 cattgcaaga atcccctgtg gcacgaacag cactagcacc gcagggattg ggccaatggc
      481 ttgcaacagg aagccggtga tagccatacc gactggttcg ctgccgaagg cgatcaggcg
      541 gaagacgctg ttgacacggc cttgcaactg gtcggggatg accgagaggc ggtagccgaa
      601 ctgcacagac atgtagattg gcacgacgat gaagctggct gcaactgcga cgcccaacac
      661 aaacggattg ggagcgacgg cgaagagcag ccaggtcagg ccccagagcc acgttgagcc
      721 aatcattagt tgaccgaagc ggaagcgccg ctggagtgga gcaacgagca ttgcgccaac
      781 gatactgcca atgccacctg acgcgaggat cagcccgatg gtgaacgacg aggcgtgctg
      841 gccctgtgcg atgacgatca ggatcaggct gtagcccgtc actggtagga tcagcccacc
      901 tgtaagcagc gcgatgaaac gaatgagcgg cgcacgccag agccacgaca gaccctcgcc
      961 gatttcggcc cacaggcgca gaggttttat atcgcgctcc tcctggaaat ccttcttgat
     1021 gaagaaaatc gagagcacgg aggcggcata ggttatggca tccgtcaaga atggaatggc
     1081 tcgagcgatt ccatagagga tgccgccaat cgacggaccg accatgccgg aaaccgagtc
     1141 gatcaccgag ttctgcgccg ccgcgcctgc gagctgctcc ttcgccacga catgcggcag
     1201 gcaggccgtc tccgccaggc cgaagaaggt gaacaaggtt ccctctacca gcgagacgat
     1261 gtagaacagc gcgatggtca tgtggccgag cagcagcgcg agcgggatgc ttcccagggc
     1321 cagcgcgcgg ccagtatcgc acaggatcat caccagcttg cgattccagc ggtccaccag
     1381 cgcccctgct ggcaggatga agagcgcgta ggggatgccg cgtattgcgc cgaccaagcc
     1441 ggcctgcgcg ggcgaatgtg tcagcgcgag gacgagcagc gggagcgcca gttgcgaggc
     1501 gcgcgtcccc gtgcttgaga cgatttgccc gctccacagc agcatataat cgcggttgcg
     1561 ccacaatggt accggtttgg cagggagggc aggtgcgcga ttctgctctc cctgccctgt
     1621 gctgtccggc ggatactgtg cctgtgacat tgttcgtact ccctgaatgc gattttgaaa
     1681 cacaaatgac caacagaacg cat
//
LOCUS       DASZAK010000427         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_615069, whole genome shotgun sequence.
ACCESSION   DASZAK010000427 DASZAK010000000
VERSION     DASZAK010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615069"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="VGT44_07480"
     CDS             complement(<1..1105)
                     /locus_tag="VGT44_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2580680.1"
                     /translation="MQHTITDDLDALLVSLPPRIHEAVNRLDNRSELLEIVMDLGRLP
                     EGRFPDGEVILSDAPVTYADLEYVVERIGEFGDDNRAGIERTLHRISALRNRKGKVIG
                     LTCRIGRAVLGSIALIRDIVEQGQSILILGRPGVGKTTLLREIARVLADEANKRVVVV
                     DTSNEIAGDGDIPHPGIGRARRMQVARTAEQHAVMIEAVENHMPQVIVIDEIGTELEA
                     AAARTIAERGVQLVATAHGNSLGNLLVNPTLSDLVGGIQTVTLGDEEARRRHTQKSIL
                     ERKAPPTFDVVVEQQSWQELVVHRDVADTVDSMLRGQLVVAEERTRDEETGRVSARRI
                     TTGGMEVPTWGIEGFGAFPRGGPDRDESDWNAMR"
     gene            complement(1117..2169)
                     /locus_tag="VGT44_07485"
     CDS             complement(1117..2169)
                     /locus_tag="VGT44_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580681.1"
                     /translation="MIRPVLYVDLPGIIYSIDRRARSRQHEFANFVCWLDPVENRVSR
                     LPILRSIFPVNPASRTWICEDHWRILGFAQVRERPSRITWDISYLASMAHRSVSSDEI
                     LTALLEYMLEIASNHAILRIFAKVEDEIPELELFQKAGFQRYARQLTYVFEPGSAQDD
                     QDRQGNFNPPTLRRWNRHFAWGLHQLYRAVTPQRVQMAEMLDSSDELAKLYVGSLRPL
                     PPALSRGDETFVCDVGVRLGAWLRLCRGHGSAPHQLSLMVHPEHADLADAILRFGTRR
                     LMEGGVRPIYCQVREYESPVINTLRSGGYEHVSTRALLVRHIALLATNPRTVPALEQR
                     VVYGVKGLGTVNTRQR"
     gene            complement(2221..>2820)
                     /gene="pdxT"
                     /locus_tag="VGT44_07490"
     CDS             complement(2221..>2820)
                     /gene="pdxT"
                     /locus_tag="VGT44_07490"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV2580682.1"
                     /translation="GVLALQGDFEAHIKALAELGVEGIPVRLPKHLTDIDGIILPGGE
                     STAIGKLMVMYGLREPLQQKIREGFPVWGTCAGLILLASESDNALAGQPLLASMHIRV
                     RRNTFGSQRESFETDLSVPALGEAERKFHAFFIRGPSIEEVGPQVEVLATLDDGTIVA
                     VREGHLLGTAFHPEIAQADGYRDTRFHRYFLRIVQSVKG"
BASE COUNT          563 a          857 c          826 g          574 t
ORIGIN      
        1 gtcgcatggc gttccagtcg ctctcgtcgc ggtccgggcc gccgcgtggg aaagcgccga
       61 agccctcgat accccaggtc ggcacttcca tgccgccggt agtgatgcgg cgggcggaga
      121 cgcgcccggt ctcctcgtcg cgggtgcgct cctcggcgac gacaagctgg ccgcgcagca
      181 tactgtccac cgtatcggcc acgtcgcggt ggacgaccag ctcctgccag ctttgctgct
      241 ccacgacgac atcgaaggtc ggcggggctt tgcgctcaag aatgctcttt tgcgtatggc
      301 ggcggcgcgc ctcttcgtcg cccagcgtga cggtctggat gccgcccacg aggtcggaga
      361 gcgtcggatt gaccagcagg ttgccaagcg agttgccatg cgcggtggcg acgagctgta
      421 cgccgcgttc cgcgatggtg cgtgcggcgg cggcctcgag ttccgtgcct atttcatcga
      481 tgacgatgac ctgcggcata tggttttcca ccgcctcgat cattaccgcg tgctgctccg
      541 ccgtgcgggc cacctgcata cggcgcgctc gcccgatgcc gggatgcgga atatctccat
      601 cgcccgcgat ttcattggag gtatccacta ccacgacccg tttattggct tcatcggcga
      661 ggacacgcgc tatttcacgc aacagggtgg ttttccccac gcctgggcga ccaagaatca
      721 agatcgattg accctgttcg acaatatctc ggatcaacgc aatgctgcca agaaccgctc
      781 gaccaatgcg gcatgtcagg ccaatcactt tgcctttgcg gttacgcagg gcgctgatac
      841 ggtgaagggt gcgctcaatg ccggcgcggt tatcgtcgcc aaattcaccg atgcgctcga
      901 ccacgtattc aaggtcagcg taggtgacag gcgcatcgct caggatcacc tccccatccg
      961 ggaagcggcc ctcgggcaag cgtccgaggt ccattacgat ctccagcagt tcgctccggt
     1021 tatccagccg gttaaccgcc tcgtggatgc gcggcggtag gcttaccagc agagcgtcca
     1081 gatcgtcagt gatcgtgtgt tgcataggct attcaatcac ctctgacgtg tgttgaccgt
     1141 ccccaggcct ttcacgccat agactactcg ctgttccagc gcggggactg ttcgcggatt
     1201 cgtcgcaagc agcgcgatat gccgcacgag caatgccctg gtcgagacat gctcgtatcc
     1261 gccggaacgg agagtgttaa tcacggggga ttcgtactcc ctcacctggc aatagatggg
     1321 gcgcacgcct ccctccatga gccgccgtgt gccgaagcgc aagattgcgt cggccaggtc
     1381 ggcatgttca ggatgaacca ttaacgagag ctggtgcggg gctgagccgt gacctcgaca
     1441 caaccgcagc caggccccca ggcgaacgcc gacatcacag acgaacgtct catcgccccg
     1501 cgagagggcc ggtggtaacg ggcgtagtga gccgacatag agttttgcta attcgtcgct
     1561 gctatcgagc atctcggcca tctgcacccg ttggggcgtg acggctcggt aaagctggtg
     1621 caatccccat gcgaaatggc gattccagcg gcgcagcgtt ggcgggttga agttgccctg
     1681 cctgtcctgg tcgtcctgag ccgatcccgg ttcaaagacg taggtcaact ggcgggcata
     1741 gcgttgaaac ccggccttct ggaagagttc caactccggt atttcatctt caaccttcgc
     1801 aaaaatacgg agaatggcgt ggttcgaggc gatctccagc atgtactcca gcaacgccgt
     1861 cagaatttcg tcgctggaga cgctgcgatg cgccatcgaa gcaagatatg aaatatccca
     1921 ggtgatgcgg gacggacgct cacggacctg ggcgaagccc aggatgcgcc agtggtcctc
     1981 gcaaatccag gtacgcgagg cggggttgac ggggaagata cttctcagga taggcagacg
     2041 agagacgcga ttctccaccg ggtcgagcca gcacacaaaa tttgcgaatt cgtgctgccg
     2101 gctacgcgca cggcgatcaa tcgagtagat gattcccggc aggtccacat atagtaccgg
     2161 tcgtatcatc cagcgctccc gcgtcgtaag gatatctgtc aggcgggaat gaaccagcct
     2221 ttatcccttg acgctttgta ctatgcgaag aaagtagcga tggaaacgag tatccctgta
     2281 tccgtcggcc tgggcgatct ccgggtgaaa ggccgtgcct agcaggtggc cttcgcggac
     2341 ggcgacgata gttccatcgt cgagcgttgc cagcacctcc acctgcggcc cgacctcttc
     2401 aatggagggg ccgcgaatga agaatgcatg gaacttcctt tccgcttctc ccagcgccgg
     2461 cacagagaga tcagtttcaa aactttctcg ctgactgcca aacgtattac gacgaacgcg
     2521 aatgtgcata gaggccagca gcggctgacc cgcgagcgca ttatcggact cgctcgccag
     2581 taagatcaat cctgcacatg ttccccagac ggggaaccct tcccgaattt tctgttgcag
     2641 cggttcgcgc aacccataca tgaccatcaa cttgccaata gcggtacttt caccacctgg
     2701 tagtattata ccatctatat cggtcaaatg tttagggagt cgtacaggaa ttccctcaac
     2761 tcccaattct gcgagcgcct tgatgtgggc ttcaaaatcg ccctgcaatg ctaagacgcc
//
LOCUS       DASZAK010000428         2608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_223745, whole genome shotgun sequence.
ACCESSION   DASZAK010000428 DASZAK010000000
VERSION     DASZAK010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2608
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223745"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1806)
                     /locus_tag="VGT44_07495"
     CDS             complement(<1..1806)
                     /locus_tag="VGT44_07495"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2580683.1"
                     /translation="MAYRFQVNQQTCINCGICMDLCPVRCLDMTRPSGEGELGLPQEL
                     LTPIPGDYAARSWMMLAPVQVAQCIGCQVCAQECPTNAIVIESSVKELAYAKPGPVSY
                     LPPEDGWQPLDAFTRAFPEEPGEQPWGEEHQWHVAERMATWQSWRTWLGERKEDLRAP
                     CQAACPVGTNAGLYVSMVAQGRYDEAFRVAYEPNPFPAICGRVCTAPCEDACRRGEFD
                     LPIAIRDLKRFASDHATQKARKITPPKKWLAERVAIVGAGPTGLSAAYYLARRGYKVT
                     VFDAMPVAGGMMSIGIPDYRLPREELNRDIDAMRELGVEIRLNTAIGLDVEFEELERE
                     YNAVLLAVGAQRSQRLGVPGEDSHGVIPATNFLKDFNLNPDTKLEGSVAVVGGGSTAM
                     DAARSALRAGASSVHILYRRTRVEMPAQAEEVRAAIAEGIELHELVAPAQILSSEGHV
                     RGVRCQRMVLSEPDEKGRRKPVPVLGTEFDIPIDSVLVAIGEAPDPSFLPEGTSVGVA
                     PWGGLLINPQTLATGAPGVFAAGDVTYGPKSIIHAAAHGRLAAKSIHSYLSHTALREI
                     TELPEDEYQTISTLPHETHITLDLRPTPREVMPLRE"
     gene            complement(2009..>2608)
                     /locus_tag="VGT44_07500"
     CDS             complement(2009..>2608)
                     /locus_tag="VGT44_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV2580684.1"
                     /translation="GQICSYIAVDHQIAGLLVLEDIPREDLSLLSSDLKAAGIKETIL
                     LTGDSEVVAHQIGEVAQVDRVLARCLPEDKLRIVQELEHQGHRVLMVGDGINDAPALA
                     TATVGMALGSQGLTAAATAADTVLLSTDILRVVKAVRLGRWVMRVALQGIWVGMGLSA
                     IAMIFAAFGFIPPAAGAILQEGIDVIVILNALRVSRIKL"
BASE COUNT          595 a          751 c          708 g          554 t
ORIGIN      
        1 ttctctcaat ggcattacct cacgtggtgt aggccgcagg tcaagcgtta tatgagtctc
       61 atgcggcagc gtagaaatcg tctggtattc atcctccggc agttcggtga tttcccgtaa
      121 agcggtatga ctgaggtagg aatgaataga tttggcggcc agcctaccgt gcgcggcggc
      181 atgaataatt gacttcgggc cgtaggtgac atcgcctgca gcaaagactc caggagcgcc
      241 tgtagccagg gtctgtggat tgatcagcag accgccccac ggggctacac cgacgcttgt
      301 tccttcgggc aagaaggatg gatcgggcgc ctcaccaatc gcgacaagca cactatcaat
      361 cgggatgtcg aattcggttc cgagcacagg gacaggcttg cggcgtccct tttcgtcagg
      421 ttcgctcaaa accatgcgct ggcagcgtac gcctctcaca tgtccttcgg atgagagtat
      481 ctgcgcgggt gcaacgagtt cgtgcaactc gataccttcg gcgattgcag cgcgtacctc
      541 ttcggcctgt gccggcatct caacgcgggt acgacgatag agaatgtgaa cgctactggc
      601 gccagcacgc agggctgaac gcgcagcatc catggctgtg ctaccaccac ctacaacagc
      661 aacactgcct tcaagtttgg tatcgggatt aagattgaag tccttgagga aattggttgc
      721 cggaatgacg ccatgcgaat cttcgccagg aacgcctaag cgctggctgc gttgcgcacc
      781 tactgccagc aaaacggcat tgtattccct ctccaattcc tcgaattcga catcgagtcc
      841 gatggcagtg ttgagccgaa tctcgacgcc gagttcgcgc atggcatcga tatcacgatt
      901 caattcctct cgcggcaggc ggtaatcggg aattccaatg gacatcatac ctcccgcgac
      961 cggcatggca tcaaagacag tgaccttgta gccccggcgc gcgagataat aggcagcgct
     1021 aaggcctgtt gggcctgcgc cgacaatcgc gacgcgctcg gcgagccatt ttttgggcgg
     1081 agtgatcttg cgcgccttct gcgtggcatg atccgaggcg aaacgcttca gatcgcggat
     1141 ggcgatgggt aaatcaaact cgccccggcg gcatgcatct tcacatggcg ctgtgcagac
     1201 acgcccgcag attgcaggga aagggttggg ttcgtaggcc actctaaacg cttcatcgta
     1261 gcgtccctgg gctaccatgc tgacatacaa gcctgcgttg gtgccgacag gacatgcagc
     1321 ctgacagggc gctcgcaagt cttctttacg ctcgccaagc caggtgcgcc agctttgcca
     1381 ggttgccatg cgttcagcaa catgccattg atgttcctcg ccccatggct gttcgcctgg
     1441 ttcttcgggg aaagcgcgtg tgaaggcatc cagtggttgc caaccatctt ctgggggtag
     1501 ataagacacg gggcctggtt tagcatatgc cagttccttg acgctgctct caatgacgat
     1561 cgcgttggtg gggcattcct gcgcgcagac ctggcaccca atgcactgcg ccacctgcac
     1621 cggcgccagc atcatccacg aacgggccgc gtagtcgcca ggaattggag taagcagttc
     1681 ctgtggcagc cccagttctc cttcgcccga cgggcgtgtc atatccagac agcgcacagg
     1741 gcaaaggtcc atacaaattc cacaattgat acacgtttgt tgattgacct gaaaacgata
     1801 ggccattccg acctcctaac aggaataatt gcatctcacc cctacgataa gcgagtattg
     1861 tgggaggagt gctggttcca tcaaacacat aagtacaatt gctcacttgt gtaagaataa
     1921 agctgtgata tcacaaatat atcacagcaa acgggaagag tatcacaaat agcacacagc
     1981 gatcagattc ttgtgatcta tggtcatatt acaatttgat cctgcttaca cgcaaagcgt
     2041 ttagtattac gattacgtca ataccttcct gcaagatagc tcccgcggcg gggggaatga
     2101 agccaaacgc ggcgaagatc attgcaatag cgctcaaccc catgcccacc caaatacctt
     2161 gcaaagccac gcgcattacc cagcgaccca gacggacggc cttgactaca cgcaggatgt
     2221 cagttgagag cagcacagta tcggcggctg tcgcggcggc ggtcaatccc tgagacccga
     2281 gggccattcc gaccgtggct gtcgccagcg cgggagcatc gttgatgcca tcgcccacca
     2341 tgagcacgcg atgaccctgg tgctccaatt cctgcacaat acgcagcttg tcctctggca
     2401 aacagcgggc gagaacccgg tcaacctgcg ccacttctcc aatctgatga gccacaacct
     2461 cactgtctcc ggtcagcaat atggtttcct tgatacccgc agccttcaag tccgatgaga
     2521 gcagcgataa atcctcgcga gggatgtcct ccaggacgag cagacccgcg atttgatgat
     2581 cgacagcgat gtagctacag atctggcc
//
LOCUS       DASZAK010000429         2673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_259890, whole genome shotgun sequence.
ACCESSION   DASZAK010000429 DASZAK010000000
VERSION     DASZAK010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2673
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259890"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2158)
                     /locus_tag="VGT44_07505"
     CDS             complement(<1..2158)
                     /locus_tag="VGT44_07505"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580685.1"
                     /translation="MPTPILATKLYVPPMRPEMVSRPRLLAQLNEGLHRKLILISAPA
                     GFGKTTLISEWLAWCERPAAWLSLDAGDSDPIRFLTYLVAALQTIAPHIGEEVPGILQ
                     SSQPAPPEAVLTALLNDLATLPDHSILVLDDYHMIDARAVDQALTFLLEHLPPQMHLV
                     IATREDPALPLARFRARDQMIEMRVTDLRFTHAETAAFLNRVMGLNLSAQDIAALEQR
                     TEGWIAGLQLAALSLQGQPDAASFIASFSGSHHFVLDYLVEEVLGRQSESIQHFLLHT
                     SILDRFCAPLCDAVVLDPSVPGQATLAYLERANLFLVPLDDERRWYRYHHLFAELLRQ
                     RLRQQPTSSPGNAESVVNELHIRASVWYEEQGLDIEAFHHATAAHDIERAARLVEGNG
                     MPLQFRGAVTPVLNWLRSLPKAVLDVRPSLWVMYASALSMTGQLTGVEEKLQAAEAAL
                     QGAELNDRTRNLIGHIAAIRALLAVAQNQVETIIAQSHRALEYLHPDNLAVRTATVWK
                     LGMAYHLQGDRPAARRAYTEAIALSEASNSIMNNVTASIGLGLIQESDTQLYAAAETY
                     RRVLHLLGESPQPGTCDAHLGLARICYEWNDLEEASQHAQQGIHLARLIENTDRVVAG
                     EVLLARLKLTGGDTAGASTLLAEAAQSAQQHNFIAPIPAVAAAQVLTLLRQGNLVAAA
                     HLAQAHDLPLGQARVYLAQGDPSEALVVLEPWRKQVE"
BASE COUNT          561 a          742 c          828 g          542 t
ORIGIN      
        1 cctccacctg ctttcgccag ggctccagca ccaccagcgc ctcggatggg tcgccctggg
       61 ccaggtagac gcgggcctgg cccaggggca ggtcatgcgc ctgcgccagg tgagcggctg
      121 ccaccaggtt gccctggcgc agcagggtca atacttgcgc ggctgccacg gcaggaatag
      181 gcgcgataaa gttgtgctgc tgcgcagact gagcggcttc ggcgagtaaa gtggacgcgc
      241 cggctgtatc tccccccgtc agtttcagac gagcgagcaa gacttcacca gcaacgaccc
      301 tatcggtgtt ttctatcagc cgcgccaggt ggatgccctg ttgtgcgtgc tgcgaagctt
      361 cttccaggtc attccattcg tagcagatgc gagccagccc caaatgcgcg tcgcaggtac
      421 ccggctgcgg cgactcacct agcaggtgca ggacgcgccg gtaggtctcg gctgctgcat
      481 aaagctgggt gtctgattcc tggatgagac cgaggccgat ggaagccgtt acgttgttca
      541 tgatactatt ggacgcctcg ctgagtgcga tggcttcggt ataggcccgc cgggccgcgg
      601 gacggtctcc ctggagatgg taagccatcc ccagcttcca gaccgtggca gtacggacgg
      661 ccaagttgtc gggatgcaga tactccagcg cgcggtgcga ctgggcaatg atggtctcca
      721 cctggttctg tgcaacggcc agcaaagctc gtatggcggc gatatgcccg atgaggttgc
      781 gggtcctgtc gtttagctcg gcgccttgca gcgctgcttc tgccgcctgg agtttctctt
      841 ccacaccggt cagttgaccg gtcatcgata aggccgaggc atacatcacc cacaacgagg
      901 gcctgacatc cagcaccgcc tttggcagcg accgcagcca gttcaggacc ggggtcaccg
      961 cgccgcgaaa ctgcaagggc atcccatttc cttccaccag gcgcgcggcg cgctctatat
     1021 catgagcggc agtcgcatgg tgaaacgctt caatgtccag gccctgctct tcataccaga
     1081 cgctggcacg aatgtgcaat tcgttcacca cgctttccgc atttcctgga gaggaggtgg
     1141 gctgctgtcg gagcctctgc cgcagcagtt ccgcgaagag atggtgatag cggtaccagc
     1201 gccgctcgtc gtccagcggg acgaggaaca ggttggcgcg ttcgagatag gccagggtgg
     1261 cctgcccggg aaccgagggg tcaagtacca cggcatcgca gagtggggcg cagaagcggt
     1321 cgaggataga ggtgtgcagc aggaaatgct gaatgctttc ggactgccgc cccaggactt
     1381 cttccaccag gtaatccagc acgaaatggt ggctgccact gaacgaggcg atgaagctgg
     1441 ccgcgtccgg ctgtccctgc agggaaagcg cggctaactg caggccggca atccagcctt
     1501 cggtgcgctg ttcaagggca gcaatgtctt gcgctgagag attgaggccc atcacccgat
     1561 tgagaaaagc ggcggtctcg gcgtgagtaa aacgtaagtc ggtgacgcgc atctcgatca
     1621 tctggtccct ggcgcggaag cgggccaatg gcagcgccgg atcttctcgc gtggcgatga
     1681 ccaggtgcat ctgcgggggc agatgctcaa gcagaaaggt gagagcctgg tctactgctc
     1741 tggcatcaat catgtggtaa tcgtcaagga cgaggatgct atggtcgggc agagtagcca
     1801 ggtcattgag caaagctgtc agaaccgctt cgggtggcgc aggctgagag gattggagta
     1861 tacccggcac ctcttctcca atgtgcggcg cgatggtctg caaggcagcc acgagatatg
     1921 tcagaaagcg gatgggatcg ctatcccctg cgtccagtga gagccaggcg gccggtcgct
     1981 cgcaccaggc aagccattcg ctgatcagcg tagtcttccc aaagccggcg ggagcagaga
     2041 tgaggatcag tttgcggtgc agtccctcgt tgagctgtgc gagcaggcgg ggacggctga
     2101 ccatttcagg ccgcattgga gggacataca gtttcgtggc gagaatggga gtaggcatgc
     2161 ctgctttgta tcctttctgc ggcctgccct gttcttcttt cctgcatgaa gcgccgcagc
     2221 tccctggctc atactcttca gggtggtttg gcaagatcat catagaggat aaaaggacat
     2281 acgtcaaggt gagattcact cgctttttgg agactcgaaa catacgaact aatccatcaa
     2341 ttgatcatga gcaacttggc tgaggtagcc caatgaagag ggttacggaa attgaggacg
     2401 aatcagtcag acatcaaaaa gggacagacg ttgagaagaa ggggtcgaga tagtagatcg
     2461 tgtgttctat ttagtttgga atattacata aataatctca gcggaaccga cgggcatcga
     2521 tccatcggtc gcgcctgtca ttcgtccctc tagagaagct tttcccaggc agaactgata
     2581 ttcttgtcta cagacatcca acagctaatg aagatgatct acttccacat tctcaacgat
     2641 acccaggttc agacccccat tggaaaatac ccg
//
LOCUS       DASZAK010000430         4103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_223780, whole genome shotgun sequence.
ACCESSION   DASZAK010000430 DASZAK010000000
VERSION     DASZAK010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223780"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VGT44_07510"
     CDS             complement(<1..1068)
                     /locus_tag="VGT44_07510"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HEV2580686.1"
                     /translation="MGNPPVRTLTLGIEQRHPLAFDTIQWADTLLRQASARYLEAGYE
                     VQTVRLSTRPVFDDLADWTDARLLGYVLELQRMLDDVGLAFCSIGPAQAARASFPLQR
                     IDVIADLLAATTAINATVQLATPEDGLRSEAALSCAQVMQRLSRETREGFGNFRFAAL
                     ACVAPGSPFFPAAYHDGRASLSIGVQGAGVITGALQSYREGNGDTTDLTSITGCVRNA
                     LFETLAPVVKIGRELATEWQIGFGGIDLSPAPLGEDSIAAALEVLGDGLIGSPGTLAL
                     AAALTSAIKSTALPTCGYCGLMLPVLEDAVLGKRWEQGTVTTHQLLLYSAVCGTGLDT
                     IPLPGDMEAREIARLLLDVAAL"
     gene            1230..>4103
                     /locus_tag="VGT44_07515"
     CDS             1230..>4103
                     /locus_tag="VGT44_07515"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEV2580687.1"
                     /translation="MNPTATPGAIKDETPSQECFLCIHDTHSCEVKQLEGLILGVRQF
                     QTGSELFPYITLDIGSSLASVRLMQSYNGLAKQLLDMGTNVRTLKLSLRILHLPQSNG
                     VTEYRGQTLTRYIANELTLAVLEPDTILNITDLNSAEYCARQYLLNRLIAMPQSAAAA
                     RGNLVHASFKELLKEYDRGELMQGHVANGEETPLATLQRHFELALEHISSDLAISNIP
                     TESMREDVAPHLESLAAWFEQQHTSLWDMPLHREASAGEGAADLPDASLVPTVRAETF
                     LLAPEIGLRGRLDLLWRQRSRQRLLELKTGGGNGDLPKSAHRRQVEGYLALLAVRRDP
                     KMKNAQATLLYSGTPGEATDFSMRFTIKQLQRVTESRNTLILSHVTGTPAPPPGPSRC
                     TKCAMRDVCTQVSSLLDWQPPQLDEDNRDQVSAARHTSAINGIPTEQDRAFFAKYYEL
                     LRLEGQEIERQQALLWTTPMQARIESGMAIGYLAPLGDVVPNGQGEWEQTFACKNTSE
                     LREGDEILLSDGNPITGEVVSGTILEISSESVKVWMRERINRPTLIDKYATDIVNVRT
                     QQNLLRWLQAETRLRLLVAGVERPRYSTDAAPALPGLNEEQRLAVERAVQMQDYLLIH
                     GPPGTGKTSVIAAIVRQLCARGQRVLLAAFTNQAVDNMLQRLEREGFRDFVRLGHDRS
                     VAEQMQPYLLKQRVRAQDTQASVQDLLRETPVIASTTATWSSDKYVPPRHAELSLQNG
                     VSTDAQADSPLRFDVAIIDEAGQLTVPAILGALRFAKRFILVGDEKQLPPLVMSEQAA
                     DAGLKDSLFSYLKQLELDYMNLHPRETSACVSLKVQYRMNSAISQFASYEYYSGQLRA
                     HPSVADRQLNYRNTGERRDGPASETRAITRALNPHPALVFLDVRDADDDQLDGPKTSS
                     AEARGVRELVAGLLARGIKEDDIGIIAPYKAQVANIRRQLFADD"
BASE COUNT          919 a         1311 c         1153 g          720 t
ORIGIN      
        1 cagcgccgcc acatccagca gcaagcgagc aatctcgcgc gcctccatat cgccgggcag
       61 gggaatggta tccaggccgg tgccacacac cgccgaatag agcaggagct ggtgcgtcgt
      121 cactgtccct tgctcccagc gcttccccag cacggcgtct tccagcaccg gcagcatcaa
      181 gccgcagtag ccacaggtcg gcaaagcggt tgatttgatc gcagacgtga gcgcagccgc
      241 cagcgccagc gtcccaggac tgccgatcag cccatctcct aatacttcca gcgccgccgc
      301 gatactgtcc tcacccagcg gcgcgggcga caggtcgatg ccaccaaagc caatctgcca
      361 ctccgtcgcc aactctctcc caatcttcac taccggcgca agcgtttcaa acaaagcgtt
      421 ccgcacacag ccggtgatgc tcgtcaaatc ggttgtgtca ccattccctt ctcgatagga
      481 ttggagcgcg ccagtgatga cacctgcgcc ctgcacgcca atcgagagac tggcccggcc
      541 gtcatggtac gctgccggaa agaacgggct gccgggcgcg acgcaggcca gcgccgcgaa
      601 acggaaattg ccaaaacctt ccctcgtttc tcgcgatagg cgttgcatca cctgtgcgca
      661 ggatagcgcc gcttcgctgc gcaggccatc ttcgggtgtg gcgagctgca ccgtcgcgtt
      721 gatggccgtc gtcgctgcca gcaaatcggc gatcacatcg atacgctgca acgggaagga
      781 cgcccgcgcc gcctgcgccg ggccaatcga gcagaaggcc agcccgacat catccagcat
      841 ccgctggagt tccaagacat accccaacag ccgggcatcg gtccaatccg ccaggtcatc
      901 gaagaccggg cgcgtcgaaa ggcgcaccgt ctgcacctca taccccgcct caagatagcg
      961 cgcgctggcc tggcgcagca atgtatcagc ccattgaata gtgtcaaacg caaggggatg
     1021 ccgctgctca atccccaacg tcaatgtgcg aactggcgga ttgcccatgg atgttacctc
     1081 gattctgtat cgtatcagga ttgtcttcac atgatacgcg cggaatctgg aagagacaag
     1141 agttcagggc aagcccacaa gagtactcct cttgacgaaa atccgttctt cacgtacact
     1201 gcctgcaaaa gagtagcgca ggagtgaacg tgaacccaac agcaacgccc ggtgcaatca
     1261 aggatgaaac accatcgcag gagtgctttc tctgcattca tgatacgcac tcctgcgagg
     1321 taaagcagct tgagggcttg attctcggtg tgcgccagtt ccaaacgggc agcgaactct
     1381 ttccctacat cacattggat atcgggtcat ccttagcatc ggtgcgcttg atgcaatctt
     1441 ataatgggct ggcaaaacaa ctcctggaca tgggcacgaa tgtgcgaacc ttgaagcttt
     1501 cgctgcgcat cctgcatctg ccacaatcca atggcgtaac cgagtataga ggccagacac
     1561 tgacacgtta tatcgccaac gagctgacgc tggctgtgct ggaacccgat acgatcctca
     1621 acatcaccga cctgaactcc gccgagtact gcgcccggca atacctgctg aaccgcctga
     1681 tcgccatgcc acagtccgca gccgcggcgc gcggcaacct tgttcacgcc agctttaagg
     1741 aactgctgaa agagtatgac cggggagaac tgatgcaggg acatgtcgcc aatggcgaag
     1801 aaacgcccct ggcaaccctg caacgccact tcgagctggc cttagagcat atcagcagcg
     1861 atctcgctat cagcaatatt ccgaccgagt ctatgcgcga agatgtcgcg ccccacctgg
     1921 aaagcctggc agcctggttt gagcaacaac acacctcgct gtgggacatg ccacttcatc
     1981 gagaagcgag cgctggagag ggcgctgccg acctaccgga tgcatccctt gttccaaccg
     2041 tgcgcgccga aaccttcctg ctcgcgcccg aaatcggcct gcgcgggcgt ctcgatttac
     2101 tatggcgaca gcgcagccgc cagcgcctgc tcgaactcaa aacgggcggc ggcaacggcg
     2161 acctgccgaa gtcggcgcat cgccgacagg tagagggcta tctcgccctg ctggccgtgc
     2221 ggcgcgaccc caaaatgaag aatgcccagg caaccttgct ctacagcggc acgccgggcg
     2281 aagcaaccga tttcagtatg cgtttcacca tcaaacagct acagcgcgtt accgagtcgc
     2341 gcaatacgct catccttagt cacgtaacgg gtacccccgc gccgccccct ggcccatcgc
     2401 gctgcacaaa atgcgccatg cgcgatgtgt gtacgcaggt ctcctcgctc ctcgactggc
     2461 agccgcccca gctcgacgaa gacaatcgcg accaggtctc cgccgccaga cacaccagcg
     2521 cgatcaatgg catcccgacc gagcaagacc gggcgttctt cgctaaatat tatgaactgc
     2581 tacgccttga gggtcaggaa atcgaacggc agcaggcgct gctgtggacg acaccgatgc
     2641 aggcgcgcat cgagtcgggc atggcgatag gctacctggc gccactcggt gacgtggttc
     2701 caaacgggca gggagaatgg gaacaaactt tcgcttgtaa aaacacatca gagctgcgcg
     2761 aaggtgacga aatcttgttg agcgatggca acccgattac cggcgaggtc gtctccggca
     2821 cgattctgga aatttcttcg gaatccgtga aagtgtggat gcgcgagcgc atcaatcgcc
     2881 ctacgctgat cgataaatat gccaccgata tcgtcaatgt gcgtacgcaa caaaacctgt
     2941 tgcgctggct ccaggcggag acacggttac ggctgctcgt ggcaggcgtc gagcgtcccc
     3001 gctactcgac ggatgcagcg cccgctctac caggtctgaa cgaagagcag cgcctggctg
     3061 tcgagcgcgc tgtgcagatg caggactacc tgctcatcca cggaccgccg gggacgggca
     3121 aaaccagcgt gatcgctgct atcgtgcggc agttgtgcgc gcggggccag cgcgtcttgc
     3181 tggcagcctt caccaaccag gccgtcgata acatgttgca acgtcttgaa cgcgaggggt
     3241 tccgcgactt cgtgcgcctc ggccacgacc gcagcgtcgc tgagcagatg caaccttacc
     3301 tgctgaaaca acgggtcagg gcgcaagata cgcaggcaag cgtacaggac ctgctgcgcg
     3361 aaacgcccgt catcgcctcg acgacggcca cgtggtcatc agacaagtat gtcccaccca
     3421 gacatgcaga actgtcgctt cagaacggcg tgtcgacgga tgcgcaggca gattcaccgc
     3481 tgcgcttcga cgtagcgatc atcgacgagg ccggccagtt gaccgttccg gccatcctcg
     3541 gtgcgctgcg cttcgcaaag cgcttcatcc tggtcggcga cgagaaacag cttccgcccc
     3601 tcgtcatgag cgaacaggcc gccgatgcgg gactcaagga ctcgctcttc agctacctga
     3661 aacagctaga acttgactat atgaatttac acccgcgaga gaccagcgcc tgtgtctcgt
     3721 tgaaggtgca ataccgcatg aacagcgcga tctcacaatt cgcctcatat gaatactaca
     3781 gcgggcagtt acgcgcgcat ccatcggtgg cggatcgcca gctgaattac aggaacacag
     3841 gagagcgaag ggacggtcca gccagcgaaa cgcgcgccat cacacgggcg cttaacccgc
     3901 atcctgccct ggtatttctc gacgtgcgag acgcagacga cgatcagctg gatggaccga
     3961 agaccagcag cgccgaggca cgcggggtgc gcgaactcgt cgccggattg ctggcgcggg
     4021 gcatcaaaga agacgatatc ggcattatcg cgccttacaa agcgcaggtc gccaatatcc
     4081 gacgccaact gtttgctgat gac
//
LOCUS       DASZAK010000431         4292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_293945, whole genome shotgun sequence.
ACCESSION   DASZAK010000431 DASZAK010000000
VERSION     DASZAK010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293945"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            437..1783
                     /locus_tag="VGT44_07520"
     CDS             437..1783
                     /locus_tag="VGT44_07520"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2580688.1"
                     /translation="MASESEQPQMGKLVGMRIREARKARNLTQSKLAGDDFSVSYISA
                     IERGQIHPSLRALEIFAQRLGLSSKDLLLASSTGGIELLESGGTNDNQELDWHLLMAH
                     VLLLQGAVTQAIQVLEQQSVKAASSWRKIRVLYLLALAHERINRWQESDYFLSEAARL
                     AKDAPAPLALRLLDLQGIVQTHIHTHSQGMALHQQCRELLETIQPADAFLKVEVYGQL
                     GYHLLQLNRIEEAREMFVTALQQTEAMTPAELAAMYYELAGQEDALERIQATLAAYKS
                     LLVSSQLSRQARFSELHHMLGRSMQHGDREQARMHLVSVLGELPSNPFVQASAHVHLA
                     AWDLDGQEVSGPGGLNGAKSHAREALTLLQATGDSIIAADACFVLARIEYAQRHYKAG
                     DLQFETGLAILERLNALEDLAERYAEFAGALELKNELHRAVHYWKKAYETRLRMKS"
     gene            complement(1786..2829)
                     /locus_tag="VGT44_07525"
     CDS             complement(1786..2829)
                     /locus_tag="VGT44_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580689.1"
                     /translation="MDKRIQNEGQPDPRQTYTPLAHWLRETLSIPTQPPSPEHTSLLE
                     TGGHPAAEYHASFYQQLPDFILALLKNDAQATLRYAPLFFHLIGCATCHTAYLELYDA
                     MRAGLAASNEPSPTWEVGTNSLALTPPRMLVYICQLLVKQADVVLTQARRNHLDHDAQ
                     ARALLQQALSMSAHIQESALRQRALQDLVRVAVLALDAGDEDAAASSHDSHTYTTLAG
                     AGSGVRQGNKTRRRAEMLEHPANDARLDLQAGPLEGMIVQEGDTLQLILRDLDEGLRG
                     RYLLIAVPLGALLEPVRWIGRNPRAIRSQSPVDEHGALKTPLGTTDLQLSNREDRNLL
                     EAMFKKLDIRSAE"
     gene            complement(2982..3884)
                     /locus_tag="VGT44_07530"
     CDS             complement(2982..3884)
                     /locus_tag="VGT44_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580690.1"
                     /translation="MPFSARDLRYHLTEHFASLYAYLQYRAKRYLGPLATDAFEVDQV
                     VGHVIEQLTRLNLLGTGDSAPKSVLDSFSDAQFYAFLNRSAQNKAIDRLRKRRAPTST
                     LTELERLGGEENELPPEDSIVQSLWGTVPFATPEEAALEAASQESLRRLLKDCIKSLH
                     DAPRQMQAVLKELADLGADDLVEEIRTESGALFDDAPLAHASQHKDHAHKKLRHCLQR
                     SSTNLAVIIALRLSEYEDVSTGSQIWTVELKTLEQDDLSEREVRMGLRHLVTEGLLDW
                     HGEESVRCSIDQRKRLARFYEEGE"
BASE COUNT          975 a         1123 c         1237 g          957 t
ORIGIN      
        1 tgggggtaga atcagccagg catttgcggc ccagggtcaa gccggggatg ttggtcactt
       61 gttgagcggc aacgacctgc cagtagacca gtccggcgct ggtggcgatg aagaatacaa
      121 taaagaggat acttaatttg cggatactgg tgcgaatttc catatttgtc ctctattctt
      181 aagatcaata aacatgtatc attgcgtggt gaagtgtatc attcttagga gcgattggca
      241 agttacctta gtaacagcta gccatgtcat catccccgat tgcacaatta cacaagaata
      301 cgataaaaaa cgcactagat aattccactc ttagatattg acaacaacca tcagcttatt
      361 ttacaatata tgcgaagagc atttttttat ttattgattc gctttcgaat atcaagacaa
      421 gtagggagta gattgtatgg cctcagaatc agagcagccc cagatgggta agttggtcgg
      481 aatgcggatc agggaggcac gtaaagccag gaatctcaca caaagcaagc tcgccgggga
      541 cgatttttcg gtcagttaca tctcggcaat cgaacgaggt cagatacatc cctcattacg
      601 cgcgcttgaa atatttgcac agcgcctggg tcttagttca aaagacttgc tgctggcttc
      661 atcaactggc ggaatagagc ttctagaatc tggtggaaca aacgacaatc aggaacttga
      721 ttggcatcta ttgatggcac atgttctact tttgcagggg gcagtcacgc aagccattca
      781 agtattagag caacagagcg taaaagcagc ctcctcgtgg cgtaaaatac gcgtcctcta
      841 cctgctcgcc ctggcgcatg aacgcatcaa tcgctggcag gaaagcgatt attttctttc
      901 ggaagctgcc cggctcgcga aagatgcgcc ggcgccgctg gccctccggc tccttgatct
      961 gcaaggcatt gtccagacgc atattcatac gcattcccag ggcatggcgc tgcaccagca
     1021 gtgccgcgag ctgctggaaa cgatacagcc ggcagatgct ttcttgaagg ttgaggtata
     1081 tggacaattg ggctatcatt tgctgcaact caatcgaatc gaagaggcgc gtgagatgtt
     1141 cgtgaccgcg ctgcaacaga cggaagcgat gacgcctgcc gagctggccg cgatgtatta
     1201 tgaacttgcc gggcaagaag acgctctcga acgcattcaa gccacgctag ctgcttacaa
     1261 aagcctgctc gtgtcgtcgc agctctcccg gcaggctcgc tttagcgaac tgcatcacat
     1321 gcttggacgc tccatgcagc atggcgatag ggagcaggcg cgcatgcacc tggtcagcgt
     1381 acttggagaa ctaccgtcca acccattcgt gcaggcaagc gcacacgtcc acctggctgc
     1441 ctgggacctg gacgggcagg aggtaagcgg gccaggcggg ctaaatgggg caaaaagcca
     1501 tgcgagggag gccctgaccc tgctccaggc gaccggggac agcattatcg ccgccgatgc
     1561 ctgcttcgtc ctggcccgaa tcgagtacgc gcagaggcat tataaggcgg gcgatcttca
     1621 atttgagacc ggcctggcaa tcctggagcg gctcaacgcg cttgaggacc tggccgaacg
     1681 atatgcagag ttcgccgggg cattggagtt gaagaacgaa ttgcaccgcg cggtacacta
     1741 ttggaaaaaa gcctacgaaa cccgcctgag aatgaagtca tagggctatt cggcagatcg
     1801 gatatccagc ttcttgaaca tagcctcgag caggttgcga tcctcgcgat ttgacaattg
     1861 caaatcggtt gtgccgaggg gtgttttgag agcgccgtgt tcatcaaccg gggactggct
     1921 gcgaatagca cgcgggtttc tgccaatcca gcgcaccggt tcaagcaggg cgccgagtgg
     1981 gacggcgatc agcagatagc gtccacgcag cccttcatca aggtctcgca agatgagttg
     2041 tagggtgtcg ccctcctgga cgatcatgcc ttcgagcggg cccgcctgca gatcgaggcg
     2101 cgcatcgttg gcgggatgtt cgagcatctc ggccctgcgc ctcgtcttgt tgccctggcg
     2161 cacaccgctg cccgcgcctg ccagcgttgt gtaggtatgg gaatcgtggg aggatgcagc
     2221 cgcgtcttcg tcgcccgcgt cgagcgcgag aacggcaacc cgcacgaggt cttgcagggc
     2281 gcgctggcgc agggcgcttt cctggatatg cgcgctcatc gagagcgcct gttgcaaaag
     2341 cgcgcgtgcc tgggcatcat ggtcgaggtg gttcctgcgc gcctgtgtca agacgacatc
     2401 ggcctgtttc accagcaact ggcagatgta gaccagcata cgaggcggtg tcagagccag
     2461 cgaattcgtc cctacctccc atgtcggcga tggctcgttg cttgcggcca gacccgcgcg
     2521 catagcgtcg tacagttcca gataggcagt atggcaggtg gcgcagccga tcaggtggaa
     2581 aaagagaggc gcgtagcgca atgtggcctg ggcgtcattt ttcagcagcg ccaaaatgaa
     2641 gtcgggtaat tgttgataaa acgatgcgtg atactcagcg gcgggatggc caccggtttc
     2701 caacagtgaa gtatgctccg gcgaaggggg ctgtgtagga atgctcagcg tctctcgtag
     2761 ccagtgcgcg agtggggtgt aggtttggcg aggatcgggt tggccctcgt tttgtatgcg
     2821 tttgtccatg tatgctcctc cagacttgct gccaaatcgc ccttattccg accggcaacg
     2881 gactgcgtcc ttcttgaaag ccgtcttgcc aacgaactac gttctagctt cgctcaggct
     2941 agacgcaaag agtgcgcttc gctcagccgg tcggaaagga attactcgcc ctcttcgtag
     3001 aagcgcgcga gtcgcttgcg ctggtcgatg gagcagcgaa cgctctcctc accgtgccag
     3061 tcaagcaagc cttccgtcac taaatgccgc agacccattc gtacctctcg ctcggaaaga
     3121 tcgtcctgtt ccagggtctt gagttccacc gtccatatct gtgagccggt agaaacatct
     3181 tcatactctg acaaacgtaa agcgatgata accgccaaat tggtactgct gcgctgtaaa
     3241 cagtgccgca gttttttgtg ggcgtgatct ttgtgctggc tggcgtgcgc gagcggagcg
     3301 tcatcgaaca acgcgcccga ttcggtgcgt atctcctcga caaggtcgtc ggcccccagg
     3361 tcggccagtt ctttcaggac cgcttgcatt tgacgagggg cgtcatgcag cgatttgatg
     3421 caatccttga gcaacctgcg taatgactcc tgggaggcag cttcgagcgc ggcttcttcg
     3481 ggtgtggcaa acgggacggt tccccataat gactgcacga tcgagtcctc cggcggaagc
     3541 tcgttctctt ctccgccaag cctttccagt tcggtcagcg tgctggtggg cgcgcggcgt
     3601 ttgcgaagtc gatcaatcgc cttattttgc gcactgcggt tcagaaaggc atagaattgc
     3661 gcgtcactaa agctatcgag gacgcttttg ggcgcgctat caccggttcc gagcagattc
     3721 agcctggtca actgctcgat tacatggccg accacctgat ccacttcaaa tgcatcggtt
     3781 gctaatggac caagatagcg tttggcgcga tattgcaaat aagcatagag ggatgcgaaa
     3841 tgttcagtta gatggtagcg tagatctctt gccgagaaag gcatgaacaa tatcctccct
     3901 gacgacagat gcgaaccagg catgcttgca ggggagccac ctgcgtattt ttaccgaccc
     3961 tgatgaatac ttttccttat tgtaacatag atttgaacgt ttgttatcga aagattctgt
     4021 aatgcagtta tctgaagttg aagtcccatt ttgcagtata ttttacatac aataactgat
     4081 gaagttccaa agggaaccag gacactgccg acgaaatcca gagggcgacc aaaatctccc
     4141 agggcgaccg caagggtcat tggctccaac taattcattt gtcgaggcga ggagaagcac
     4201 gagaggagga agcaggcgag cgccgcggct tcttgcgagg cgaccgatgg gggcgctgct
     4261 tgtaggtgag atagcacgcc agacgggtac ga
//
LOCUS       DASZAK010000432         1883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_579035, whole genome shotgun sequence.
ACCESSION   DASZAK010000432 DASZAK010000000
VERSION     DASZAK010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579035"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..1698
                     /locus_tag="VGT44_07535"
     CDS             352..1698
                     /locus_tag="VGT44_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HEV2580691.1"
                     /translation="MLFYGFTLEDHVPEDHLLRSIDRFVELSGVRRQLEPYYSAIGRP
                     SIDPELMIRMLLIGYCCGIRSERRLCQEVSLNLAYRWFCRLELTDPVPDHSTFSKHRH
                     GRFRDSDLLRKLFEASVRRCMAEGIVGGEGFAVDASMIRADAHRQHGVATSAELDPQH
                     ATRALNEYLAVLDDAAFGAATDVAPKFISPVDPAARWTAAASGPACYAYCDNYLIDLK
                     CAVIVDVEPTTAVRQAEVTAAKTMIIRTAETFGLEPERLAADAGYGSAEMLAWLVHEQ
                     GIEPHVPVFDKSERKDGTFARDDFHYDHEGDVYTCPAGKQLRKYRRPFAAPRIGVTKA
                     NTVLYRASKFDCDACSLKPKCCPNTPARKVPRSIHEGARDMARDIAKTDAYVVSRRQR
                     KKVEMLFAHLKRIIRLDRLRLRGPCGARDEFHLAATAQNLRKLATLIPMSKPIPAT"
BASE COUNT          371 a          638 c          533 g          341 t
ORIGIN      
        1 ccactgatcg tcacgcagtc cgaaccgccg catcggtcag ccccccacaa gctgaccgcc
       61 tatgaatcga agaaatcgcc gcgccggaat cggctccgta tcgccccgcg tcgattgacg
      121 gctgagtgtg gcaatcggaa ctcaccggcc cattacggtg cgcgggtgac ccccaccgac
      181 cgagatcggc taattgatga caggccctaa taccaacggc cttttccggg gaccgcttgg
      241 ggtggattcc gttgaaaaag tcttgcttga agtcgttctg gtgacgtgat tcaatcctcg
      301 gcggcagccg cggaatacag gcatgatggg cgcgcgaaca gtgagacaag gatcctgttc
      361 tacggcttca ctctggaaga tcacgtccct gaagatcacc tcctgcgctc gatcgaccgc
      421 tttgttgaac tgtcaggtgt ccgccggcaa ctggaaccct actacagcgc gatcggacgg
      481 ccatccatcg atcccgagtt gatgattcgg atgctgctca tcgggtattg ctgcggcatt
      541 cgctcagagc ggcggctttg ccaggaagtc tcgctgaacc tcgcctatcg ctggttctgc
      601 cgtctcgaac tcaccgatcc cgtgccggac cattcgactt tctctaagca tcggcatggc
      661 cggttccgcg acagcgacct gctgcgcaag ctgttcgagg ccagcgttcg gcgatgcatg
      721 gccgaaggca ttgtcggcgg ggagggcttc gcggtcgatg ccagcatgat ccgcgccgat
      781 gcccatcgtc agcatggcgt agccacatcc gccgagcttg acccgcagca cgccacccgc
      841 gccttgaacg agtatctggc ggtgctcgac gatgccgcgt tcggtgccgc gaccgatgtc
      901 gcaccgaaat tcatctctcc cgtcgatcct gccgcgcgct ggactgcggc cgctagcggt
      961 ccggcctgct acgcctattg cgacaactat ctgatcgacc ttaagtgcgc ggtcattgtc
     1021 gacgtcgagc ccaccactgc cgtgcgccaa gcggaagtca cggcggccaa gacgatgatc
     1081 attcgaaccg ccgagacgtt tggcctggag ccggagcgtc tcgcggccga cgccggctac
     1141 ggatcggctg agatgctcgc atggctcgtc cacgaacagg gcatcgagcc gcacgtcccg
     1201 gtgtttgaca agtcggaacg caaggacgga acctttgcgc gcgacgactt tcattacgac
     1261 cacgaaggtg acgtctacac ctgcccagcc ggcaagcagc tccgcaaata ccgtcgcccg
     1321 ttcgcagcac cacggatcgg cgtgacgaag gccaacaccg tgctctaccg cgccagcaag
     1381 ttcgactgtg atgcctgctc cctgaagcca aaatgctgtc ccaacacgcc agcccgcaag
     1441 gtgccgcgca gcatccatga gggtgcgcgc gacatggctc gcgacatcgc caagaccgac
     1501 gcctatgtcg tctcgcggcg ccagcgcaag aaggtcgaga tgctgttcgc gcacctcaaa
     1561 cgcatcatca gactggaccg actgcgatta cgaggaccct gcggtgctcg cgacgagttc
     1621 cacctagccg ccaccgccca gaacctccga aagttggcaa cgctgatccc gatgtccaag
     1681 ccgataccgg ccacctaacc cgggtggccg catctgcccc ctttaggtca aatatgcggc
     1741 ccctcacaca ccgaacccgc aaccgacttt atcaacggaa tccacccatc gcagacattc
     1801 caaattgagt gcggcgcttg ggcgacttcc tagtcaccga actgaccgac gaggccgcgc
     1861 ctgcatccgc ggcatcccag ccg
//
LOCUS       DASZAK010000433         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_45282, whole genome shotgun sequence.
ACCESSION   DASZAK010000433 DASZAK010000000
VERSION     DASZAK010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45282"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="VGT44_07540"
     CDS             <1..229
                     /locus_tag="VGT44_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV2580692.1"
                     /translation="LRKQPPFQAALRGINAAVVGLLLAALYTPVWTSTIKAPIDFLFA
                     LAAFGLLVVWKCPPWLVVLLAALVGAGLSLL"
     gene            complement(691..3303)
                     /locus_tag="VGT44_07545"
     CDS             complement(691..3303)
                     /locus_tag="VGT44_07545"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2580693.1"
                     /translation="MKKSSLAKPNLRLKEVRELRGWSQKYVADGIGAERYYLSRWEHG
                     AASPSPYYRQKLCILFGMNARELGLLPEDHADELESQVEQLAATPVQVAPVSAESASR
                     VFDPTLPTPLMGAARLVGRDEIVQQLRQRLCTGKGVVLTAINGLPGVGKTTLAVELAH
                     DEQIQEHFQHGVLWAGLGPTPDVFAILSHWGTLLGIPPLQMIKLSSIDEWSRALRTAI
                     GQRRLLLVIDDAWRIEAALAFKVGGPHCAYLVTTRFPNIALHFTYEAGEAIVLKELSI
                     DEGLALLARLAPEVVRSEPQSARALVQAVGGLPLALTLMGKYLRVQEHGNQPRRIRAA
                     LERLRTANERLRLTEPLSPLERPVGLDPSAPLSLQTVIAVSDQHLEDQEQHALRALSI
                     FPAKPNSFSEEAAQAVSAVPVETLDALSDAGLLENSSPGRYTLHQTITDYAQVQLTDP
                     SVYERMANFFAAFVEQHEKDFDLLDIESNNIFAALDVAYTAGNRRSYLRCINAFFLFL
                     FTRSLHNEETGTYLEKALPMARQENDTILLAMTLLARGKLIYRQGNYAQAEADLLEAH
                     ELVKPAGNAKLLSEALMMLGILARFHVSYDRAEAYFSQSLELARRVNDPELVSDVLSR
                     LGGMFSDRGRYAEAEAYNQEGLELARAVGNTNRVAKLVSNLSTLAILQGKFEQAERYG
                     LEALELARQVGSLDEICYISTNLGALALDMQEYTKAERYLKESLAIARRIADMKIISA
                     DLGSLGTLAMRLERYDEASAYLQESLALARQVGDTWLVSSILNERGELALKQQHLEEA
                     SAAFSEASSISAQGNQSEVAAALFGLARVAAERGDIVQARRLGRECLDILQPTGNRWN
                     EAVQAWYDALPGEA"
     gene            complement(3411..4169)
                     /locus_tag="VGT44_07550"
     CDS             complement(3411..4169)
                     /locus_tag="VGT44_07550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEV2580694.1"
                     /translation="MYIKFSLEEKFSETGGKRPYVRRLFGRIARVYDPMNRLMSFGQD
                     RRWRAFAARYLALGTGQLGLDLGTGTADLSIAVIRRSGPGTRMIGMDITPEMLEVGRR
                     KLERMGLQDVIDLRVGDAEHIDLPDDCVDGCCSAFTVRNLTDIRQGFREMLRVVRPGG
                     RVVCLEISHPPGAVFGALFHFYFYKLAPLFGTILGKAAEEYNYLPQSLTNFPDAPSLK
                     RIMEEVGWSDVRFYRLTRGIVAVHVGTNALAPQE"
BASE COUNT          885 a         1301 c         1189 g          914 t
ORIGIN      
        1 gctacgcaaa cagcccccgt tccaggcagc cctgcgcggc atcaacgccg ccgtcgtcgg
       61 gctgctgctg gcggcgctct acacccccgt ctggaccagc accatcaaag ctcccatcga
      121 cttcctgttc gctctggctg ccttcggcct gctcgtggtc tggaaatgcc cgccctggtt
      181 ggtcgtgctg ttggccgcgc tcgtgggagc gggattgagc ctgctgtaat ttcatgcggc
      241 cccatcgtag ggacacgatg atgagtgttc acgaccaagt tcggcaacca cgtctgtcat
      301 cctgagtgca gcgaaggatc tcggtgtcca gcgcatcaga cccttcgcta cgctcagggt
      361 gacaacccct gaccgatctt ggtgtgaaca cacgtcatcg cgtccgggtg gaccagctca
      421 accgatgttg aggacgcgat gatgaagtta cacgaccaaa gccggtaagt gcccgtgtcc
      481 gtaggggcga tcccttgtgg tcgcccgccc gtgatgttgg ccgcttctga cacccgatcg
      541 atcaaagcgg gcgggcgacc acaagggatc gcccctacgg acacgattac cgatctcgtt
      601 tgtgaaagtt caatatcgcg tccctacgac gtgaatcatt cttctgcatg atcttgatgc
      661 gcttcggata ttagcggcag aagcgttggc ctatgcctct ccgggcaagg cgtcgtacca
      721 tgcctggaca gcttcgttcc agcgattgcc tgtcggttgc aggatatcca ggcattcccg
      781 gcctaaacga cgcgcctgca cgatgtcgcc tcgttctgcg gccactcgcg ccaggccaaa
      841 gagcgcggca gccacctctg attggttgcc ctgtgcagat atcgacgacg cttctgaaaa
      901 agctgcggag gcttcttcga ggtgttgttg cttcaaggca agttcgccgc gttcgttcag
      961 gatggagctg accagccagg tatcacccac ctgccgcgcc agggcgagcg actcttgcag
     1021 gtaggctgac gcctcgtcgt agcgttccag gcgcatagcc agtgtgccga gcgagccgag
     1081 gtcagcgctg atgatcttca tatcagcgat acggcgcgca attgccagcg actccttcag
     1141 atatcgctca gctttggtat attcctgcat gtcgagggcg agcgcgccca ggttggtaga
     1201 gatgtagcag atttcatcca gcgatccgac ctgtcgcgcc agttccagcg cctccagtcc
     1261 atagcgctct gcctgctcga atttcccttg caggatcgcc agggtactca agttgctgac
     1321 cagtttcgcg actctatttg tattgccgac agcgcgcgcc agttccagcc cctcctggtt
     1381 atacgcttcg gcctcagcat agcggccccg atcgctaaac atgccaccaa gccgggagag
     1441 gacatcgctg accagttcag gatcgttgac ccgccgcgcc agctccaggc tctggctaaa
     1501 ataggcttcc gcgcgatcgt aggagacatg aaatcgagcg agaataccca gcatcatcaa
     1561 ggcctcgctc aggagctttg cgttgcccgc tggcttcacc agctcgtgcg cctccaacag
     1621 atcggcctcc gcctgcgcat aattgccctg ccggtagatc aatttccctc gagcgagcag
     1681 cgtcatggcc agcaagatcg tatcattttc ctggcgagcc atcggcaacg ctttttcaag
     1741 ataggttccg gtttcttcat tgtgcaggct gcgcgtgaaa agaaaaagga aaaaggcatt
     1801 gatgcatcgc aaataactgc gacgattgcc agcagtgtag gcgacatcca gggctgcaaa
     1861 gatattgttc gattcaatat caagcaggtc aaagtctttt tcgtgttgct ccacgaacgc
     1921 agcaaaaaaa ttcgccatgc gttcgtagac ggatggatcg gtgagttgta cctgggcata
     1981 gtcggtgatg gtctggtgca gagtgtagcg accggggctg ctattttcca gaaggccagc
     2041 gtcgctgagg gcatccagcg tttcgaccgg aactgcgctt accgcctgag ccgcctcctc
     2101 cgaaaagctg ttcggcttgg cgggaaaaat cgaaagcgcc cgcagggcgt gttgttcctg
     2161 gtcttccagg tgttgatcgc tcacagcaat cacggtctga agcgagagtg gcgctgacgg
     2221 gtcgagacca accgggcgtt cgagcggcga cagcggctcg gtgaggcgca ggcgttcgtt
     2281 ggccgtgcgc aggcgttcaa gggcggcgcg aatgcggcgc ggctgattgc catgctcctg
     2341 aacgcgcagg tatttgccca tcagggtcag tgccagcggc agccctccga cagcctgcac
     2401 cagcgcgcgc gcactctgcg gttcgcttcg caccacctcc ggcgcgagcc gcgctaacaa
     2461 cgccagccct tcatcgatgc tcagttcttt gaggacaatg gcctcgcctg cttcatacgt
     2521 aaagtgcaag gcgatattgg ggaagcgcgt cgtcaccagg tacgcgcagt gtgggccgcc
     2581 caccttaaaa gccagtgcgg cctcgattcg ccatgcgtca tcaatcacaa gcagcagccg
     2641 tcgctgtccg atagcagtgc gcagcgcgcg cgaccattca tcgatactgc tcagctttat
     2701 catctgcaaa ggagggatgc caagcagcgt tccccaatgg cttagtatgg cgaaaacatc
     2761 gggtgttggg ccaagtccgg cccagagaac gccatgctgg aaatgttcct ggatctgctc
     2821 atcatgtgcc agttcaacgg ccagcgtcgt tttaccaaca cccggcagcc cattgatggc
     2881 cgttagtaca actcccttgc ccgtgcacag ccgttgccgc agttgttgga ctatctcgtc
     2941 gcgcccaacc agccgcgcag cacccatcag cggagtcggt agcgtcgggt cgaatacccg
     3001 cgaggcactt tcggctgata ccggcgcaac ctgaacaggg gtggcggcca actgctcaac
     3061 ttgagactct agctcatcag catgatcttc cggcagcaaa cccaactcac gcgcattcat
     3121 gccaaagagt atacatagct tctggcgata gtaggggctt ggcgaggcgg cgccatgctc
     3181 ccaccggctc aaatagtagc gttccgcccc gatcccgtcg gccacgtact tctgcgacca
     3241 gccacgcagc tcgcgcactt ctttcaggcg tagattcggc tttgccagcg acgatttttt
     3301 catggtcggg cgattccaga aacaggatta tagcagcaaa ttcaaaacaa agaataccat
     3361 aaacaagttt gtgtacgttt cctttgtttg taaaggctat aataccccct ttattcctgg
     3421 ggtgcaagag catttgtacc gacatgcact gctacaatgc cgcgcgtcag acgatagaaa
     3481 cgcacgtccg accaccctac ctcctccatg atacgtttca gtgatggagc atcgggaaag
     3541 ttggtgagcg actgcggcag atagttgtat tcttcggcgg ctttgccgag gattgttcca
     3601 aacaggggag ccagtttgta gaagtagaag tggaagagtg cgccaaagac cgcgccgggg
     3661 ggatggctga tctccaggca gaccacgcgc ccgcccggcc tgacaacgcg cagcatctcg
     3721 cggaatccct gccggatatc ggtcaaattg cgcacggtga atgcggaaca acacccatcg
     3781 acgcagtcgt ccggcaggtc aatatgctct gcatcgccca cacgcagatc gatcacgtcc
     3841 tgcaaaccca ttcgttccag cttgcgccga ccgacctcca gcatctccgg cgtgatatcc
     3901 atgccaatca tgcgtgttcc tggccctgaa cggcgaatga ccgcgatcga cagatcagcc
     3961 gttcccgtac cgagatccag ccccaactgg cccgttccca gcgccaggta acgcgccgca
     4021 aaggctcgcc agcgccggtc ctggccgaag ctcatcagcc ggttcatcgg atcatacacg
     4081 cgagcgatac gcccaaagag tcgcctgaca tagggacgtt tgccgcccgt ttcgctaaat
     4141 ttttcctcca gggagaattt gatatacact ttgatcttcc ttcatgctat cttttatatt
     4201 gtattataag cgtccaattc ctcatacatc cagtgatagt aagtaacctg taggagccgg
     4261 tttaccgcgc tcaccgccga ttgatcggc
//
LOCUS       DASZAK010000434         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_685700, whole genome shotgun sequence.
ACCESSION   DASZAK010000434 DASZAK010000000
VERSION     DASZAK010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685700"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..828)
                     /locus_tag="VGT44_07555"
     CDS             complement(37..828)
                     /locus_tag="VGT44_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2580695.1"
                     /translation="MPMIHGLYYEEQGSGSETIVFAHGLLWSGRMFDDQVAALKERYR
                     CVAFDFRGQGQSEVTRDGYDMETLYEDAAALIETLQCAPCHFVGLSMGGFVGLRLAAR
                     RPELVRSLILLETSADPEPQETVPKYRQMSIVARWLGPGVVADRVMPIMFGQKFLTDP
                     ARAELRREWRQRMASNHRVGVSRATMGVITRRGIRDELAQIRVPTLIIVGDQDVAVPL
                     VHSEHLHEGIAGSRLVIIPGAGHTSTVEEPDAVNAAISEFLESQR"
     gene            1060..2094
                     /locus_tag="VGT44_07560"
     CDS             1060..2094
                     /locus_tag="VGT44_07560"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEV2580696.1"
                     /translation="MLPERGARMLHITNGESTQGSLQQSGIPGDVLAWKDVLHEGPVP
                     ANLSLAQMSQVRARFIADSYKLPYDEVVQSFTQRDRTLARFRNFEEVVFWFEHDLYDQ
                     LQLIQILDWFAEQKPGSTRLSLICINTFPGIDHFMGLGQLTPAQLASLFDTRQSVTRS
                     IFDLSSRAWNAFCSPTPTAIETLVQSDTTDLPFLAHALTRHLEEFPATDTGLSRSEKQ
                     ILEVIDSGIQHPGEIFRASLKKEDAAFMGDLTFFKRVTNLCENPHPLLQRVDSSAFSL
                     PMDGMYDKAFRDQALVLTSDGQAALLGRVDWIALRGGIDQWLGGTHLQGRRGLWRWDV
                     EQRRLRLIEE"
     gene            2097..2432
                     /locus_tag="VGT44_07565"
     CDS             2097..2432
                     /locus_tag="VGT44_07565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2580697.1"
                     /translation="MSTAFDHIEVRNNEDEQQYEVTIDGHTAILFYEREGNSITFLHT
                     EVPPALEGHGIASTLAHTALEDARTLGLNVVPLCPFVASYIRRHPDYLSLLTEAEQAR
                     LRKRGEDQA"
     gene            complement(2423..>3253)
                     /locus_tag="VGT44_07570"
     CDS             complement(2423..>3253)
                     /locus_tag="VGT44_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580698.1"
                     /translation="PNAVALNSSLATLARIVGPGIAGIIIAASSVTILFLLNAFSFLA
                     VLVGLALIDSHELHAQAHQHSDAGERQNTWQSLREGVAYVWKTPAVLLAIIVVGLVLL
                     FGSNFNVFLPLFATDVLHVGATGFGFLSASIGVGALCSALWLAWSNSAPTIRKMLIGT
                     LVFGVLEAAFAVSHIYLLSLALIASVGFAETAFAQLSLTTLQTVAPDHLRGRVMSVQV
                     LFFDGSVPLGYLLVGWLSGLYGASLAMLICAFLSLAIAAVGWMWHKKAAIHVAGPRQA
                     "
BASE COUNT          758 a          937 c          895 g          663 t
ORIGIN      
        1 ataagcacta cttgtaggca attccccgat ggaaagtcag cgctgacttt ccagaaactc
       61 actgattgcc gcattgactg cgtcaggctc ctcgaccgtc gaagtatgac ccgcgccggg
      121 aataatgacc aaccgtgagc cagcaatccc ctcgtgaaga tgctcggagt ggaccagcgg
      181 cacagccaca tcctgatctc cgacaataat cagcgtcgga actcgaatct gcgctagctc
      241 gtcgcgaatt ccacggcggg taattacccc catcgtcgcg cgcgaaacgc cgacgcggtg
      301 attgctggcc atgcgttgcc gccactcgcg ccgcaattcc gcgcgcgccg ggtcggtgag
      361 gaacttctgg ccgaacatta tcggcatgac ccggtccgcc acgacgcctg ggccgagcca
      421 acgggcgaca atgctcatct gcctgtactt cggcacagtc tcttgcggct caggatcggc
      481 ggaggtctcc aggaggatga gcgatctgac cagttccggt cgccgcgccg ccagccgcag
      541 tccaacaaag ccgcccatcg acaggcccac aaaatggcag ggcgcgcatt gcaacgtctc
      601 gatgagcgcc gccgcatcct cgtacagcgt ctccatatcg tagccgtcgc gcgtcacctc
      661 gctctgcccc tgcccgcgaa agtcgaacgc cacgcagcga tagcgctcct tcaacgcggc
      721 cacctggtca tcaaacatgc ggccactcca cagcagccca tgcgcaaata cgatggtctc
      781 ggagccgctg ccctgctcct cataatacaa cccatgaatc atcggcatcg tctctgctcc
      841 tttgcactat tctccattgc ttggttactc attgcaatag atgcattagt atatcccata
      901 ggcctgccgt tcaggatgat tcccaggtat ggatgaacaa gtggtaggta aaaacaaaaa
      961 ctcgtaggag ccgataaatc ggctcctacg gggttcgtca agacgcaagg ttgataattc
     1021 aagaacgcat gtgctattct tacaggtagg aagacgatta tgttaccgga gagaggtgcg
     1081 cgaatgctgc atattacgaa tggggaaagt acccagggaa gtctacagca atcaggtatt
     1141 ccaggtgacg tgctggcctg gaaggatgtc ctgcacgaag ggccggtgcc agcgaatctc
     1201 tcgctcgcgc agatgagcca ggttcgcgct cgcttcattg ccgactccta caaattgccc
     1261 tacgatgagg tcgttcagag ctttactcaa agagatcgta cccttgcgcg ttttcgcaac
     1321 tttgaagagg tggtcttctg gttcgagcat gatctgtacg accagttgca actcattcaa
     1381 atcctggact ggtttgcgga acaaaagccg ggatcaacac gtttgagcct catttgtatc
     1441 aacacttttc ccggtatcga ccacttcatg ggcttaggcc agctcacccc ggcccaactc
     1501 gcttccctct tcgatacacg acaatcagta acgcgctcta tctttgattt gagcagccgt
     1561 gcatggaatg cgttttgttc accaactcca actgcgattg aaacacttgt ccagagcgat
     1621 accaccgatt tgcctttcct ggcacatgcg ctgactcgac accttgagga atttccagca
     1681 accgataccg gtctttcccg cagtgagaag cagattctcg aagttatcga ttcaggcata
     1741 cagcatcctg gcgagatatt tcgggcctct ttgaagaaag aagatgccgc gtttatgggc
     1801 gacctgacgt ttttcaagcg cgtaacgaac ctctgtgaga accctcatcc tctgctccaa
     1861 cgtgttgata gtagcgcctt ctctttgcct atggatggca tgtatgataa ggcgtttcga
     1921 gaccaggcgc tggttctcac cagtgatgga caagctgcct tgcttggccg cgtcgattgg
     1981 attgcgctgc gcggtgggat tgaccagtgg ttgggcggca cacatttaca gggacgacgg
     2041 ggtttgtggc gttgggatgt ggagcagcga agactgagac tgatagagga gtgaacatga
     2101 gtacggcatt cgaccatatc gaggtcagga ataacgaaga tgagcagcaa tacgaagtaa
     2161 cgattgatgg acacaccgcg atacttttct acgagcgcga aggaaatagt attacgttcc
     2221 tgcatactga agtgccgccg gctctggaag gtcacggcat cgcgagtacg ctggcgcata
     2281 ccgccctgga agacgctcgc accctgggac tgaacgttgt tcccttgtgt ccctttgtag
     2341 catcgtacat tcgacgccat ccagactacc tgtcgctgct gaccgaggcg gaacaggcca
     2401 ggctacgcaa gcgcggtgag gatcaagcct gacgaggacc ggcaacatgt attgctgcct
     2461 ttttatgcca catccacccg actgccgcga tcgccaggct gagaaaagcg cagatcagca
     2521 tggcgaggga agctccatag agacctgaca gccagccaac cagcagatac cccagcggga
     2581 cgcttccatc gaaaaagagt acctgcacgc tcatgacgcg cccgcgcagg tggtccgggg
     2641 cgaccgtttg gagcgtcgta agagaaagct gtgcaaaggc ggtctccgca aagccaacgc
     2701 tcgcgatgag cgctaatgac aggagataga tgtgcgagac cgcaaaagct gcctctaaca
     2761 ccccgaagac cagcgtaccg atcagcattt tgcggatcgt tggagcgcta ttgctccagg
     2821 ctagccacag cgccgagcag agcgcgccaa cgccgatcga cgcagagaga aagccaaagc
     2881 ccgttgcgcc cacatgcagc acgtcggtgg cgaagagtgg cagaaagacg ttgaagttcg
     2941 agccgaagag cagcaccagg cccactacga tgatcgctaa caataccgca ggcgttttcc
     3001 agacataggc cacgccttcg cgcaggctct gccaggtatt ttgccgctcg cccgcgtcgg
     3061 agtgttgatg tgcctgggcg tgtagctcgt gactgtcaat cagcgccagt cccaccagca
     3121 ccgcgagaaa gctgaacgca ttgagcagaa agagtatagt cacggagctt gcggcaatga
     3181 tgatgcccgc tatgccaggg cccacaatgc gcgccagcgt agcgagggaa gagttcaggg
     3241 caaccgcgtt ggg
//
LOCUS       DASZAK010000435         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_685704, whole genome shotgun sequence.
ACCESSION   DASZAK010000435 DASZAK010000000
VERSION     DASZAK010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685704"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /gene="cofC"
                     /locus_tag="VGT44_07575"
     CDS             <1..535
                     /gene="cofC"
                     /locus_tag="VGT44_07575"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEV2580699.1"
                     /translation="LVLDMLAHVLSILHSCERFERISVVSSDWRALSFAEQRGAMPVL
                     EQQRGHNPALRAAAQRELDDGAQALLTISADLPLLDRCDILSMIALAEQFDVVLAPSH
                     EGTGTNALLTKPPLVIPYLFGENSSARHLEAARTRHINSTLYQSSTLAFDVDTIEDVQ
                     QLERVRPMWREQPGLAV"
     gene            609..1016
                     /locus_tag="VGT44_07580"
     CDS             609..1016
                     /locus_tag="VGT44_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV2580700.1"
                     /translation="MAAKLSDKARDFLRGKRFAVLATLNRDGTPHLTTMWYLLEEDGT
                     IMMNTKAGRVKDRHMRRDPRISVCVAEGYSYITLSGKVEMIEDQEIAQQDIFRLSSRY
                     HGLDKAKIQMQNQFSKEQRVTLRLKPDHVIEYF"
     gene            1028..1921
                     /locus_tag="VGT44_07585"
     CDS             1028..1921
                     /locus_tag="VGT44_07585"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEV2580701.1"
                     /translation="MTRTYGSQDMFSPLRTVLLKRPDDAFAVDDPAVWHYAGKPDLAA
                     AQQEHDNLAAILRQAGAEVVYHDEPLPNHADAIYAFDPALVTDAGAVILSMGKQLRRG
                     EEEAMARCFERMSVPILARLHGEARAEGGDLLWLDHDMLAVGIGFRTNAEGFRQLREV
                     LSAIGVEVLPFELPYYTGSEACLHLLSLISIVDHRTAVVYPPLMAVPFWQELERRNFK
                     LIVIPEAEFSTMGTNVLALAPRKCLVLEGNPVTKALLEEASCEVITYRGNEISLKAEG
                     GPTCLTCPVWREYLSRLFHFL"
     gene            complement(1895..>2413)
                     /locus_tag="VGT44_07590"
     CDS             complement(1895..>2413)
                     /locus_tag="VGT44_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580702.1"
                     /translation="GKVVFDGYMAALMLAKAREDRPALDIAASVLDRMLPDDADTKDF
                     LTAMRNDDLGKLNMDNVNAGVTGLILSWFSEANLAAPPRLMQISVFQNDMTTWCVNIA
                     QAILEDDNEQLAHAIDEAEAHHLVVHAARMRVVLAQRTHDRAQMERARLVLERLQDRR
                     FLNRLQEVEEAR"
BASE COUNT          517 a          684 c          692 g          520 t
ORIGIN      
        1 gttagtgctg gatatgctcg cgcatgtcct gtcgatcttg cactcttgcg aacgcttcga
       61 gcgcatcagc gtcgtcagct cggattggcg ggcgttgtcg tttgccgagc agcgtggcgc
      121 gatgcctgtg cttgaacagc agcgcggcca caaccctgct ctacgcgctg ctgcccagcg
      181 cgaactcgac gatggcgcac aggccttgct gacaatctcc gccgacctgc cgttgttgga
      241 tagatgcgat attctgtcga tgatcgcgct agctgagcaa ttcgatgtcg tgcttgcgcc
      301 atcacatgaa ggcaccggga cgaacgccct gctgacaaag ccgccgcttg ttatccctta
      361 tctcttcgga gagaacagca gcgcgcgaca tctcgaggct gccagaacgc ggcacattaa
      421 cagcacgctg tatcaaagct ccacgctggc ctttgatgtc gatacgattg aggatgtgca
      481 acagttggaa cgagtgcggc ccatgtggcg agagcagcct ggactggcgg tgtaatgccg
      541 gttaacctgg cgtttcgtat agagataaaa ttgcacagag atcaaaagga gtaacacaaa
      601 cattcatcat ggcagcaaaa ttatctgaca aagcccgtga cttcctgcga gggaaacgtt
      661 tcgcggttct cgctacgctc aacagggacg ggacaccgca tttgaccacc atgtggtatc
      721 tcctggaaga ggatggcact atcatgatga atacgaaggc cgggcgcgtc aaagatcgcc
      781 acatgcggcg tgacccgcgc atctctgtct gtgtcgcgga aggctatagc tatatcactt
      841 tgagtgggaa ggtcgagatg atcgaggatc aggagattgc ccagcaagat atctttcgtc
      901 tttcttcgcg ctatcatggc ctggataagg caaaaatcca gatgcagaac cagttctcta
      961 aagagcagcg cgtgacgctg cgcctgaagc cagatcacgt tatcgaatat ttctaggagc
     1021 tacaaccatg actcgcacat acggcagcca ggacatgttt tcgccactgc gtaccgtgct
     1081 cctaaagcgg cctgacgatg ccttcgcggt agacgatcct gccgtgtggc actatgccgg
     1141 aaagcccgac ctggcggcag cgcagcagga gcatgacaac ctggcagcga tattgcgtca
     1201 ggccggggct gaggttgttt atcacgatga acctctgccc aatcatgccg acgccatcta
     1261 tgcctttgat ccggcgctcg tgaccgacgc tggcgcggtg atattgagta tgggcaaaca
     1321 actgcgacgc ggcgaggaag aggcaatggc gcgctgcttc gagcgtatga gtgtgccgat
     1381 cctcgctcgc ttgcacggcg aggcgcgcgc cgaaggcggc gatctgctct ggctcgacca
     1441 tgacatgctt gcggtcggca tcggctttcg caccaacgct gaaggctttc gccagttgcg
     1501 cgaagtcctc tccgccatcg gcgtcgaggt tctgccgttt gagctaccct actacaccgg
     1561 ctcggaggcg tgtctgcacc tgctctcgct catcagcatc gtcgatcacc gcactgccgt
     1621 cgtctaccca cctctcatgg cggtcccatt ctggcaggag ttagagcgaa ggaatttcaa
     1681 actgattgtc attcccgagg cggaattctc gacgatgggg acgaatgtgc tggcgcttgc
     1741 cccgcgtaaa tgcctggtgc tggaaggcaa cccggtgaca aaagcattgc ttgaagaggc
     1801 aagctgcgag gttataacct atcgcggcaa cgaaatttct ctcaaagccg agggcggacc
     1861 aacgtgcctg acgtgcccgg tttggcgtga atatttatcg cgcctcttcc acttcctgta
     1921 agcgattgag gaaccggcga tcttgcaggc gctccaggac gaggcgggcg cgttccatct
     1981 gcgcgcggtc atgggtgcgc tgggcaagca ccacccgcat tcgcgcggcg tggacgacga
     2041 ggtgatgcgc ctctgcctcg tcaatggcat gcgcaagttg ttcattatcg tcttctagaa
     2101 tggcctgagc gatgttgacg caccaggtcg tcatatcgtt ctggaagacg gagatctgca
     2161 tcaaacgcgg gggagccgcc aggttagctt cactaaacca cgataagatc agccctgtca
     2221 cacccgcatt gacgttatcc atgttgagct tgcccagatc gtcgttgcgc atagctgtca
     2281 gaaaatcctt tgtatcagca tcatcaggca gcatacggtc gaggaccgag gcagcgatat
     2341 ccagtgcagg tcgatcctca cgcgcttttg ccagcatcag cgcagccatg tagccatcga
     2401 aaaccacctt tcc
//
LOCUS       DASZAK010000436         6462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_508240, whole genome shotgun sequence.
ACCESSION   DASZAK010000436 DASZAK010000000
VERSION     DASZAK010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508240"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VGT44_07595"
     CDS             <1..534
                     /locus_tag="VGT44_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580703.1"
                     /translation="LSATTTRKANYAVLGGSVSDFTKVFGKPANDGGTPTTYLDYQPY
                     RPPWNIIYKITISIDIGVDGNYFVSGIIFNPDLNNLIRWQDGINFCQSFLPADAIHLS
                     DTDFAPPNQFYQQMYLSKNMAHEFGADHFMQGAGLQQTPVAPGTVQIMYLYTSSSNHT
                     SREARIRVCYISLGQPY"
     gene            complement(723..796)
                     /locus_tag="VGT44_07600"
     tRNA            complement(723..796)
                     /locus_tag="VGT44_07600"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Val,seq:tac)
     gene            complement(841..922)
                     /locus_tag="VGT44_07605"
     tRNA            complement(841..922)
                     /locus_tag="VGT44_07605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(887..889),aa:Leu,seq:gag)
     gene            complement(995..1405)
                     /locus_tag="VGT44_07610"
     CDS             complement(995..1405)
                     /locus_tag="VGT44_07610"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEV2580704.1"
                     /translation="MAIDSSLIGATSSPVEFEVTREAVERFMRATEDPALQGDTEPEY
                     APPTFPTTFRMRVPGLELDGSKMQLIHGEQRYTYTRLLRIGETVTCQASVADIRERAG
                     KSGSMTFVVTEITGQDSQQQPVFTARSTLIVRQK"
     gene            complement(1530..2108)
                     /locus_tag="VGT44_07615"
     CDS             complement(1530..2108)
                     /locus_tag="VGT44_07615"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV2580705.1"
                     /translation="MPEYQPWTVLATYQVLDTPYLKIRREEVSVPDGPIIPDYYIIEN
                     RGWVGVVPVTEDGRLLINKQYKHGIGLEVIEFPAGGIDAHEDDPLETARRELMEETGY
                     SFTDDNVEQLAHFYANPTGARTLVWWYLARNVRQTGTPKPDPVEVIENELVTPAELLA
                     MIHNGTFCVQGQVGAAYMALERLGYLTFSGQR"
     gene            complement(2118..2903)
                     /locus_tag="VGT44_07620"
     CDS             complement(2118..2903)
                     /locus_tag="VGT44_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580706.1"
                     /translation="MQQAYSAIRVISEAQTVRIVLSTHPGESMLKELCAACASLNSPG
                     SEGIKAVVLDFSSPGQVDAADRDSHANAEQSYRAIHAVAQPVLAVARDSLSPAACDLL
                     AAADLTLVNAEAMLMLASAKQHDEGQHSEAGEAVESLLGAQAARLGYVTWAAAAGDLN
                     REMERILKLLREKSAVALRLAKAGARLGQAEQAKQATPLEVYAAGRRRTFVQQAEQAK
                     QATPLEALHLINALYLAEVMQTQDAAEGLRAFLEKRKPVWKNR"
     gene            complement(2907..4481)
                     /locus_tag="VGT44_07625"
     CDS             complement(2907..4481)
                     /locus_tag="VGT44_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEV2580707.1"
                     /translation="MTIPTVTLPDQFNVASAFLDRNIQEGRGNNIAIYYEGQSYTYAQ
                     VAELANRAGNGLLDLGVDMEQRVALLLLDSPQFAAAFFGAIKIGAVPIPINTSLRPAD
                     YVYMLNDSRARVLLVHAAIWQTMAQILPDLKYLRHVVVVGLEASGQQASATLHDFERW
                     VGKSSSSLQAAETSKDDSALWLYSSGSTGFPKGCVHLQHDMVYCTEYYAKPVLGIRED
                     DITFSAAKLYFAYGLGNNLYFPFGVGASAVHYAGRPLAEDMYKVVQQYRPSIFFGVPT
                     LYAAMLALPDAEKRFDFSSVRICVSAGESLPADILRRWQDKFHVDILDGIGSTEILHI
                     FISSRAGDIRPGSTGKLVPGYEALIADETGHAVKQGDIGNLLIRGDSTAAYYWNKHEK
                     TKDTITGHWIHTGDKYYQDEDGFFWYCGRSDDMLKVSGQWVSPVEVEAALIAHPAVLE
                     SAVVGDTDADELVKPRAYIVLNQGFAPSGELADELKAFIKQRLAPHKYPRWIDFVVEL
                     PKTATGKIQRFKLREH"
     gene            complement(4525..5271)
                     /locus_tag="VGT44_07630"
     CDS             complement(4525..5271)
                     /locus_tag="VGT44_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV2580708.1"
                     /translation="MTINKERLDVALVRRGLAPSRERARALIMAGQVYVGSALADKPG
                     TMIALDAECRLAETPDELKYASRGGLKLEKALDSFGLNPTGLVALDVGASTGGFTDCL
                     LQHGARRVYALDVGRGQLAWTLRRDPRVIVMESTNIRHVTALPEVVQCAVIDVSFISL
                     RLVLPPLAPLLAPGAWVVALVKPQFEAGKAEVDRGEGVIRDPAVHERILAELQAWIPQ
                     HTPFTIRGLTDSPIYGRDGNREFLLWLCLG"
     gene            complement(5258..6373)
                     /locus_tag="VGT44_07635"
     CDS             complement(5258..6373)
                     /locus_tag="VGT44_07635"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV2580709.1"
                     /translation="MASTPGTPTTDAAQSPLVIGVDIGGTQIRAAVLQGATLHSRANA
                     ITAGQTAPDVMLPRVFALIDQALQEAQVTLDQVASIGISTPGPVNNRTGIVYEPPNLP
                     GWIQLPLRDIFLQRYSLPIRVENDANTAALGEYLFGAGRGCQHMIYMTVSTGIGGGVI
                     IDGKIVEGSIGTAGEIGHMSIDWHGPVCNCGNRGCLEYFASGPAIARRANEAIKAGQG
                     AELLAFASAMLDHPANVPDRSALPVPQDLNTQPLEQAGQPGEALHVNARTVARAAEAG
                     IPLARSIIAEVAEALGVGLVNLLHLFNPDMIILGGGVALRLGDMLMEPALRIVQERAM
                     AAPRQSVQITLAQLGDNAGLIGAGALTQYYEQLHDHQ"
BASE COUNT         1218 a         1928 c         1960 g         1356 t
ORIGIN      
        1 ctatctgcta ctacaacaag gaaagccaat tacgccgttc tcggcggcag tgtgagtgac
       61 ttcaccaaag tattcggtaa accggcgaac gatggaggca ctcctactac atatttggat
      121 taccaaccgt atcgcccgcc atggaacatc atctataaaa ttactatcag catagacata
      181 ggagtagacg gcaactattt cgtttctggc atcatcttca acccagatct aaataatctg
      241 atacgttggc aagatggcat caatttttgc cagtcgtttc tgcctgctga tgcaatccac
      301 ctgagcgata ctgactttgc tccgccgaat caattctatc agcaaatgta cctcagcaag
      361 aacatggctc atgagtttgg tgctgatcat tttatgcaag gcgccggctt acaacaaacg
      421 ccggtagctc ctggtacagt acaaatcatg tacctgtaca caagttcctc caaccacacg
      481 agtagagaag ctcgaatcag ggtctgttat atatcactcg gtcaaccgta ttaaaaattt
      541 gagcggagcc aacctgagcg aggcttacaa ccctatcaat taaccgagaa aagcatgctg
      601 aaaacaggca ataggcttga caccgccaaa tcgggtacga agcctactgc ttagctcaac
      661 tgttggaata ccgctcgatt gttctagagc ggcttcacag gccgatgtag agacaagaga
      721 agtgggcgac tagggactcg aaccccagac ctactgcgtg taaagcagcc gctctaacca
      781 actgagctag tcgcccgcct gagtggggat tgtttgggtt gtcggttgta tttgtagggg
      841 tgccctcgag tggactcgaa ccaccacgcc tttacaggca caggccctca acctgctgcg
      901 tataccattc cgccacgagg gcaagttctt ccgtaacgga ttacgccgcc tattgtatac
      961 ctgtccttgc gctttgtcaa taagttttgc gagcttattt ttgccgcacg atcagcgtgc
     1021 tgcgggcggt gaagacgggc tgctgctggc tgtcctggcc ggtgatttcc gtcacgacga
     1081 aggtcatcga gccgcttttg cccgcgcgct cgcggatgtc ggcgacgctc gcctggcagg
     1141 tgacggtttc gccgatgcgc agcagccgcg tataggtata gcgctgctcc ccgtggatca
     1201 actgcatctt cgagccgtcc agttccaggc cggggacgcg cattcggaac gtcgtcggga
     1261 aggtcggagg cgcgtattca ggttctgtat cgccctggag cgccgggtct tcggtggctc
     1321 gcatgaaacg ttccaccgcc tcgcgtgtca cctcgaactc aacgggcgac gaggttgcgc
     1381 cgatcaggct gctatcaatt gccatgtgtg ttgctccttt ctgtcatgct tcgcttcgct
     1441 cagcatctcg cgaccatcga ctgagaccct tcgcttcgct cagggtgacg ggctataacc
     1501 taattggcac ctacggttgg gttatgatat caacgttgtc ctgaaaacgt gagatatccc
     1561 agccgttcca gcgccatgta cgcggctcct acctggccct ggacgcagaa ggtgccgttg
     1621 tggatcatgg cgagcagttc ggcgggcgtg accagttcgt tttcgatgac ctcgaccggg
     1681 tcgggtttgg gcgtgccggt ctgacgcacg ttgcgcgcca ggtaccacca taccagcgtg
     1741 cgcgcgcccg tcgggttggc gtagaagtgc gcgagttgtt cgacgttgtc gtcggtgaag
     1801 ctgtagccgg tctcctccat cagttcgcgt cgggcagtct ccagcggatc gtcctcgtgg
     1861 gcatcgatgc cgcctgccgg gaattcgatc acctcaaggc cgatgccgtg cttgtattgc
     1921 ttgttgatga gcaggcggcc atcttccgtg accggcacga cgccgaccca gccgcgattc
     1981 tcgatgatgt aatagtcagg gataatgggg ccgtcaggaa cggatacctc ttcgcggcgg
     2041 attttgagat agggcgtgtc aagcacttga tacgtggcga gaactgtcca gggctggtat
     2101 tctggcatag agtttcctta tctattcttc caaactggct tgcgtttctc caggaaggcg
     2161 cgcagaccct cggcggcgtc ctgtgtctgc atcacctccg cgagatagag cgcgttgata
     2221 agatgcagcg cttccagcgg cgtcgcctgc ttagcctgtt ctgcctgctg aacgaatgtt
     2281 cgccttcgac cggcggcgta aacttccagc ggcgtcgcct gcttagcctg ttctgcctgc
     2341 cccaggcgcg cgcccgcttt ggccaggcgc agggcgaccg cacttttttc gcgcagcagc
     2401 ttcaggatgc gctccatttc gcgattgagg tcgcccgccg ccgctgccca ggtcacatag
     2461 ccgaggcgcg ccgcctgcgc tcccagcagc gattccaccg cttctcccgc ctcgctgtgc
     2521 tggccctcgt cgtgctgctt cgcgctggcg agcatcagca ttgcctcggc gttgaccagc
     2581 gtcaagtcgg cggctgccag caggtcacac gcggcaggcg agagggaatc gcgcgcgacg
     2641 gccagcacgg gctgcgctac ggcatggata gcgcgatagg attgttcagc gttcgcgtgg
     2701 ctatcgcggt ctgccgcatc cacctggccg gggcttgaga agtcgaggac gaccgccttg
     2761 atgccctcgc tgccggggct attgagcgag gcacaggcag cgcacagctc tttcagcatc
     2821 gactcgcccg gatgcgttga gaggacgatg cgcaccgttt gcgcctctga gatgacgcgg
     2881 atggccgagt aggcttgttg catggcctaa tgctctctca gcttgaaacg ctggatttta
     2941 cccgttgcgg tcttgggcag ctcgaccacg aagtcgatcc agcgcgggta cttgtgagga
     3001 gccaggcgct gcttgatgaa ggctttcagc tcatcggcca gctcgcctga tggcgcgaag
     3061 ccctggttca ggacgatgta ggcgcgcggc ttgaccagct cgtcggcatc cgtatcgccc
     3121 acaaccgcgc tttccagcac ggcgggatgg gcgatcagcg cggcctctac ctcgaccggc
     3181 gagacccact ggccgctgac cttgagcata tcgtcggagc gaccgcagta ccagaagaaa
     3241 ccgtcctcgt cctggtagta cttgtcgccg gtatgaatcc agtggccggt tatggtgtct
     3301 ttggtcttct cgtgcttgtt ccagtagtag gcggcggtgc tgtcgccgcg aatgagcagg
     3361 ttgccgatat ctccctgctt gacggcatgg cctgtttcgt ccgcgataag agcttcgtag
     3421 cctggcacga gcttgccggt gctgcccggc ctgatgtcgc ccgcgcggct gctgatgaag
     3481 atgtgcagga tttcggtgct gccgataccg tccaggatat cgacgtggaa tttgtcctgc
     3541 cagcgccgca ggatatcggc gggcagcgat tcgcccgccg agacgcagat gcgaaccgag
     3601 gagaagtcga agcgtttctc ggcgtccggc agggccagca tcgcggcata gagcgtcggc
     3661 acgccgaaaa agatgctggg gcggtactgc tgcaccactt tgtacatatc ctccgcgagt
     3721 ggtcggccag cataatgtac cgcgctcgcg cccacgccga acgggaagta gaggttgttg
     3781 cccaggccat aggcgaagta gagcttcgcg gcggaaaaag tgatatcgtc ttcgcgaatg
     3841 cccagcaccg gtttggcata gtattcggtg cagtagacca tgtcgtgctg caaatgcaca
     3901 cagcctttcg ggaaaccggt gctgcccgaa ctatagagcc acagcgcgct atcgtctttg
     3961 ctggtctcag ccgcttgcag gctggatgat gacttgccga cccagcgctc gaagtcgtgc
     4021 aaggtcgcgc tggcctgctg accgctggcc tccagcccga cgacgactac atggcgcagg
     4081 tacttgaggt ctggcaaaat ctgcgccatc gtctgccaga tggcggcgtg gacgagcagg
     4141 acacgcgcgc ggctgtcgtt gagcatgtag acgtaatcgg cgggtcgcag cgaggtattg
     4201 atcggaatgg gtaccgcgcc gatcttgatc gcgccgaaga aggcggcagc gaattgtggc
     4261 gagtcgagta gcagcaaggc cacgcgctgc tccatgtcaa caccgagatc gagcagccca
     4321 ttgccggcgc ggttagccag ttcggcaacc tgcgcgtagg tgtagctttg cccttcgtag
     4381 taaatagcga tgttgtttcc tcgcccttcc tggatattgc gatcgagaaa ggcgctcgcg
     4441 acgttgaact ggtccggcag cgttaccgtt gggatagtca taaggcacac ctcgtttcat
     4501 aaaagcgtaa atcctgttgc tattttaccc caaacacagc caaagcagaa actcccggtt
     4561 gccgtcgcgt ccatagatcg gcgaatcggt caggccgcgt atcgtaaatg gtgtgtgttg
     4621 cggtatccat gcctgcaatt cagccaggat gcgttcatgc actgccgggt cgcgaatgac
     4681 gccctcgccc cggtctacct cggcctttcc tgcctcgaac tgcggcttga ccagggcgac
     4741 cacccaggcg ccgggcgcga gcaggggcgc gagcgggggt agcacgagcc gcaaggaaat
     4801 gaaggagaca tcgatcacag cgcactgcac cacttcgggc agcgcggtga cgtggcgtat
     4861 gttggtcgat tccatcacaa tcacgcgcgg gtcccggcgc agcgtccagg ccagttgccc
     4921 gcgcccgacg tccagagcgt agacgcgcct cgcgccatgc tgtaggaggc agtcggtaaa
     4981 gccgccggtt gacgcgccga catccagcgc gaccagaccc gtcgggttca aaccaaaact
     5041 gtccagcgct ttttcgagtt tgaggccgcc gcgactggcg tatttcaact catcgggggt
     5101 ctcggccagg cggcactccg cgtcgagcgc gatcatcgtt cccggcttat cggccagcgc
     5161 gctgcccacg tagacctggc cagccatgat gagcgcgcgg gcgcgttccc ggctgggcgc
     5221 aaggccgcgc ctcaccaacg ccacatctaa ccgctcttta ttgatggtca tgcaactgct
     5281 cgtaatactg cgtcaatgcg ccagctccga tcaggcccgc gttatcgccc aattgcgcca
     5341 gggtgatctg gaccgactgg cgcggcgcgg ccatagcgcg ttcctgtacg atgcgcaggg
     5401 ctggttccat cagcatatcg cccaggcgca gggcaacgcc gccgcccagg ataatcatgt
     5461 cggggttgaa gaggtgcagg aggttcacca gtcccacgcc gagcgcctcg gcgacttcag
     5521 cgatgatgga acgcgccagg ggaataccgg cctccgccgc gcgcgcaacc gtccgcgcat
     5581 tcacgtgcaa tgcttcacca ggctggcctg cctgttccag gggttgcgtg ttcaagtctt
     5641 gaggaacggg cagggcagag cggtccggca cgttggcggg gtggtcgagc atggcgctgg
     5701 cgaaggccag cagttcggct ccctggccag ctttgatggc ttcattcgcc ctgcgagcga
     5761 tagcgggtcc agaggcgaaa tattccaggc agccgcgatt cccgcaattg cagacgggac
     5821 cgtgccagtc gatcgacata tggccaattt ctcccgccgt gccaatgctc ccttcgacga
     5881 tcttgccatc gataatcaca ccgccgccga tgccggtgct gaccgtcatg taaatcatat
     5941 gctggcagcc gcgaccggcg ccaaacaggt actcacccag cgccgccgtg ttcgcgtcat
     6001 tctccacgcg gataggaagc gagtagcgtt gtaagaagat atctcgcagc ggcaactgta
     6061 tccagcctgg caggttgggt ggttcataga caatgccggt gcggttgttg accggcccag
     6121 gcgtggaaat gccgatgctg gcaacctggt caagtgtcac ctgggcctct tgcagcgcct
     6181 ggtcgatgag ggcgaagacg cgcggcagca tcacgtccgg cgcggtctgt cctgccgtga
     6241 tcgcattcgc gcgagaatgc agcgtcgcgc cctgcaacac ggcggcgcgt atctgcgtcc
     6301 ccccgatatc gacgccgatg accagcggcg actgcgccgc gtcggtcgtg ggtgtaccag
     6361 gtgtgctagc catagaatat ccctactaat ccttctttgc cgtggacaac tttcgtaggg
     6421 acagggaggc ggcgtggagc ggcgggggcc ttgcgcctgt cc
//
LOCUS       DASZAK010000437         1902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_82358, whole genome shotgun sequence.
ACCESSION   DASZAK010000437 DASZAK010000000
VERSION     DASZAK010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82358"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VGT44_07640"
     CDS             complement(<1..831)
                     /locus_tag="VGT44_07640"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2580710.1"
                     /translation="MQNNPAPRDALTTGDTQGRTSEQVLLVTKLARPSVGADLVARPR
                     LTDLLQPGIQRPLTLIAAPAGFGKTTLLSTWLEHAPLRSAWVSLEHDDDDLIRFWSYV
                     ITALDLVHAGSGESALALLQGSAPQQPPPIETVLTVWINGLAALSHEVVLVLDDYHLI
                     TAPPIHRSVAYLVDHQPPQLHLVIATRADPPLPLARLRTSGHLTEIRAADLRFTSHET
                     AAFLTRSVGLNLSSEDIAALEARTEGWIAGLQLAALSMRGRQDIPAFLKAFTGSQRYI
                     I"
     gene            complement(905..1531)
                     /locus_tag="VGT44_07645"
     CDS             complement(905..1531)
                     /locus_tag="VGT44_07645"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEV2580711.1"
                     /translation="MSQNAKKTILSRIRAANASAPDARPTPRDYRERDERERAAIIED
                     VIDRLLDYKAIVTRADRAVLPQAIADACRQHDIARLVVPADIPADWIPDDVTALRDDP
                     PLAVGDLDTSDGVLTGCALAIAQTGTIILDGGALQGRRALSLVPDRHLCVVFAEQVVG
                     LVPEAIAGLAERATRPITLISGPSATSDIELSRVEGVHGPRTLHVFVV"
     gene            complement(1528..>1902)
                     /locus_tag="VGT44_07650"
     CDS             complement(1528..>1902)
                     /locus_tag="VGT44_07650"
                     /inference="COORDINATES: protein motif:HMM:NF023298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HEV2580712.1"
                     /translation="EHAKSLPYASTLCGACYEVCPVSINIPEVLLHLRGKVTQQASPL
                     NPEALAMRQLARVFASPKRYEQAQRLARRGQRFFMHGDVIDHLPGMLKGWTTARDLAP
                     IPKASFRDWWRARQQQEKGGQA"
BASE COUNT          378 a          544 c          637 g          343 t
ORIGIN      
        1 gataatgtag cgctgactac ccgtgaacgc tttgagaaat gcgggaatat cttgccgccc
       61 ccgcatcgac agcgcggcta actgcaagcc cgcaatccag ccctccgtcc gcgcctccaa
      121 agcagcgatg tcctcacttg aaagattcag gccgacagaa cgagtcagga aggcggccgt
      181 ttcatgagaa gtgaagcgga gatcagccgc gcggatttcg gtcaaatgac cacttgtgcg
      241 caggcgcgcc aggggaagag gaggatcggc tcgtgtcgca atgaccaggt ggagctgggg
      301 cggctgatga tcgacgagat aggcgacgga tcgatggatg ggcggcgcgg tgatcaggtg
      361 gtagtcatcc aggacgagaa ccacctcgtg ggacagggct gcgaggccat tgatccagac
      421 ggtcaagacg gtttctatgg gtggcggctg ttgaggagcc gatccttgaa gcagtgccag
      481 ggcggactca ccactgccag catggacaag atcaagagca gtgatcacat aagaccagaa
      541 gcgtatgagg tcgtcatcgt catgctccaa agagacccag gcagatcgaa gaggagcgtg
      601 ttcaagccat gtggaaagga gggtcgtctt gccaaagccg gcaggggctg cgatgagtgt
      661 gagcgggcgc tggatgccag gttggagcag gtccgtcagg cgcgggcgag caaccaggtc
      721 ggcaccgacc gagggcctgg cgagtttcgt gaccaggagt acttgctcgc tcgtgcgacc
      781 ttgcgtgtct cccgttgtca gagcatctcg tggggcagga ttgttttgca tcggctctgc
      841 actcccacag tactatttcg aaggctggct tattctgcga ttaactatag cacacccacc
      901 ggcactacac tacaaacaca tgcagggtgc gcggaccatg cacgccctct acgcggctca
      961 actcgatatc gctggtggca gatgggccgg aaatgagggt gatagggcgt gtggcgcgtt
     1021 cggccagacc agcgatggct tcgggaacca gcccgaccac ctgttcagcg aagacgacgc
     1081 acagatgccg gtcgggaacg agcgagagcg cgcgccgtcc ttgcaatgcg ccgccatcca
     1141 ggatgatcgt acccgtctgg gcgatggcaa gcgcgcagcc cgtcagcacg ccgtcgctgg
     1201 tatccaggtc gccaacggcg agcggcgggt cgtcgcgcag ggcagtcaca tcgtcaggaa
     1261 tccagtcggc gggaatatca gccggaacga ccagccgcgc gatgtcatgc tgacggcagg
     1321 catccgcgat ggcctggggc aggacagccc gatccgcgcg cgtcacgatg gctttgtagt
     1381 cgagcaggcg atcaatcaca tcttcgatga tggcggcgcg ttcgcgttcg tctcgctcgc
     1441 ggtaatcacg cggcgtcggg cgcgcgtcgg gcgcgctggc attggcggcg cggatacgcg
     1501 acaggatggt ctttttcgcg ttctgactca tgcctggccg cctttctcct gctgctggcg
     1561 cgcccgccac cagtcgcgga agctcgcttt gggaatggga gcaaggtcgc gtgcagtcgt
     1621 ccagcctttg agcattcccg gcaggtggtc gatcacatca ccgtgcatga agaaacgctg
     1681 tccgcgccgc gccaggcgtt gggcctgctc gtagcgtttc gggctggcga agacgcgcgc
     1741 cagttgtcgc atcgccagcg cctccgggtt gagcggcgat gcctgctgcg tcactttgcc
     1801 gcgcagatgc agcaatactt cagggatgtt gatgctgacg ggacagactt catagcaagc
     1861 cccgcacaaa gtggaggcat acggcagcga tttggcatgc tc
//
LOCUS       DASZAK010000438         1610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_650441, whole genome shotgun sequence.
ACCESSION   DASZAK010000438 DASZAK010000000
VERSION     DASZAK010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650441"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VGT44_07655"
     CDS             <1..194
                     /locus_tag="VGT44_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2580713.1"
                     /translation="ELFGVWGAVLAAPLAGLIQALLVAIWQNWRETHPEQFDSEEEAV
                     AAPDAPAQIVAPEKEEILE"
     gene            complement(200..859)
                     /locus_tag="VGT44_07660"
     CDS             complement(200..859)
                     /locus_tag="VGT44_07660"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580714.1"
                     /translation="MSGLHIALREDEGLSLIFMKTNEESATALARVAQRIRQHVRQSD
                     AVLLGERECAIILPRTSLASAQAVARRVALLLVDIECEIELLYGPAAYTLIQRLQSSG
                     VIVAQREEVVDSFAYLPALTQTSDPSLPYLAFLADYPSRRLFRLFPYDLACQYQCFPV
                     GAERDMLTVGTRERLDENVVAHFKQVTKRGIFQVRCEERLIDSVLRYWQRLFEVEAMF
                     N"
     gene            1373..>1610
                     /locus_tag="VGT44_07665"
     CDS             1373..>1610
                     /locus_tag="VGT44_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2580715.1"
                     /translation="MTARQKSRLVSRRGRGELAVQRLQQLTQTLSRPAPPLVVTTLHF
                     SVIVCTYNRRRLVLNTLASLRRQTLPFSAFEVIVV"
BASE COUNT          378 a          450 c          433 g          349 t
ORIGIN      
        1 ctgaactttt cggcgtctgg ggcgctgtgc tggccgcgcc cctggcgggt ctgatccagg
       61 cgctgctcgt cgccatctgg cagaactggc gtgaaactca ccccgagcag ttcgatagcg
      121 aggaagaagc tgtcgcagcg ccagatgcgc ccgctcagat tgtggcgccc gaaaaagagg
      181 aaatcctcga ataaaaccgt cagttgaaca tggcctctac ctcaaacagg cgctgccaat
      241 agcgcagcac gctatcgatc aggcgctcct cgcagcgcac ctgaaagatg ccgcgcttcg
      301 tcacctgttt gaaatgcgcg acgacgttct cgtccagccg ctcgcgcgtc ccgactgtca
      361 gcatatctcg ctcggccccc accggaaaac actgatactg gcaggccagg tcgtagggga
      421 acaggcggaa caggcggcgc gatgggtaat cggctagaaa tgccaggtac ggcagcgaag
      481 ggtcgcttgt ttgcgtaaga gccggcagat acgcgaatga gtccaccacc tcttcacgct
      541 gagctacgat aacgcctgac gactgtaacc gttgtatcaa cgtatacgca gccgggccgt
      601 agagcaactc tatttcacac tcgatatcca cgagcagcag ggcgacgcgc cgcgcgactg
      661 cctgcgcgct cgccagcgat gtgcgcggca gaatgatggc gcattcacgc tcgccaagta
      721 aaacagcgtc actctgccgg acatgctggc gaatacgctg cgccacccgc gccagcgcgg
      781 tcgcgctctc ctcgttcgtt ttcatgaaaa ttagcgaaag gccctcatcc tctcgcaacg
      841 cgatatgtaa tccactcaac ccaacatcct ccacatactt ataaatagac ccttatcgta
      901 ggagccgatt tatcgcgctc accgccgatt atgaactttc acaatcaaga tcggcaagag
      961 ctgtcatcct gagcgcagcg aaggatctcg gtgtccggcg tgtaagatcc ttcgctgcgc
     1021 tcaggatgac attaccggac ttgatcgtta aaaatcatta tcggccagga atggactatc
     1081 ccgacggttg gacaggccga tcaatcggct cctacggttt agattacatg atagctgcgt
     1141 tcgctcggaa tgttcttgtc aagtggttga gatattgtta agactctgca atagtggttg
     1201 agaaaacgac tatattaata attttcgata cttaacatca atttgggaaa agttaccgag
     1261 ttgcaaccct gatattacgg gaattcctat ggtcggtttg catactatat agtagaaggt
     1321 ggaacagtgg ctgtgaataa ggcaacacac ctggataaaa gagtatgacc gaatgactgc
     1381 acggcaaaaa tcacggttgg tgtcgagacg gggcagggga gaacttgccg tccagcgatt
     1441 acagcaattg acgcagactt tgtcgcgtcc cgcgcctcct ctcgttgtta caacgctgca
     1501 tttcagtgtg atcgtctgca cctacaatcg tcgccggcta gtcttgaaca cgttggcgag
     1561 cctgcggcgg cagaccctgc ccttcagcgc ctttgaggtg attgtcgtgg
//
LOCUS       DASZAK010000439         2307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_615225, whole genome shotgun sequence.
ACCESSION   DASZAK010000439 DASZAK010000000
VERSION     DASZAK010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615225"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2282)
                     /locus_tag="VGT44_07670"
     CDS             complement(<1..2282)
                     /locus_tag="VGT44_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEV2580716.1"
                     /translation="MSLDCYLGPRKSMLTETALHAKLLHLETPLLNESQLETMRNLKD
                     FPMQTLDVTFKSAGGPDALEAALDRLEHEAVAAVAQGIALLVLNDQSASPAQPPVPML
                     LAVGAVHQALIRHGQRTQISLICETGSVWDVHQIALLLGYGAEAVVPTLALASVRAMA
                     GERHLEHLTREEAAERYLHVIEGGLRKVMARMGISTLRNIVGAGQFEAVGLEPQLIER
                     CFAGSVAHPGSVTCAHIAQQVIEGCQALQEEASPPVVSNGRRRKLIDFGRYRFRRSGE
                     YHAYHPLLIRALQKAAQTGNMDDYRQFTQMVYQRPPTALRDLLSFVPTTPIPLEQVES
                     MESIRSRFVISAMSLGALSPETHRTIAAAMNSIGGRNNTGEGGENPDWYHEQLNGYPL
                     GSKIKQVASARFGVTTEYLVRAEELEIKMAQGSKPGEGGQLPPKKVTPFIARLRHTAP
                     YVALISPPPHHDIYSIEDIAQLIYDLKQVNPRARVGVKLVSSIGVGTIAAGVAKAHAD
                     YVQISGHDGGTGASPLQSIKHAGIPWERGLAETQQVLVRNGLRGRVKLRVDGGLKTGR
                     DVVIGAMLGAEEFGFGTASLVALGCDMARQCHLNTCPTGIATQREDLRAKFTGTPQML
                     IHYLTLVAEEVRELLARLGVARLDDLVGRADLLQCSGEARVELEPLLVAAPVASSHHA
                     IGIKLPQSPLAGQLLVEAEQALSGERSVLTQHHIHNYDRTIGAGLAGEIARRYGNAGL
                     PGVSITSVFHGAAGQSFGAF"
BASE COUNT          489 a          667 c          662 g          489 t
ORIGIN      
        1 caaaatgcgc caaaactctg cccggccgct ccatgaaata cactggtaat gcttacacct
       61 ggcaggcctg cgttgccgta gcggcgtgcg atctcccctg ccaaaccagc cccaattgtg
      121 cgatcatagt tgtggatgtg atgctgagtc aacacactgc gttcaccaga gagagcctgt
      181 tccgcctcga ccagtaactg cccggcaagc ggcgattggg gcagttttat gcctatggca
      241 tgatgagagg aggctacggg cgcggcaacg agcaggggct caagctcaac gcgtgcctcg
      301 ccgctgcatt gcaggaggtc agcacggcca accagatcgt ccaggcgtgc cacgcctaac
      361 cgcgccagca attcgcgaac ctcctcagct accagagtga gatagtgaat aagcatttgt
      421 ggcgtacccg tgaattttgc acgcaggtct tcacgttggg tggcaatacc tgttgggcaa
      481 gtgttcaggt ggcactggcg cgccatatca cagcccagcg cgaccaggct ggcggtccca
      541 aaaccaaatt cctcggcccc caacatcgcg cctatcacga catcgcgccc cgtcttcaga
      601 ccgccatcga cacgcagctt cactcgtccg cgcaggccgt tacgcaccag gacctgctgt
      661 gtttctgcta atccacgctc ccagggaata cccgcgtgct tgatcgattg cagtggtgag
      721 gctcccgtac ctccatcatg tccactaatc tggacatagt cagcatgggc cttcgcaacg
      781 cctgctgcga tggtgccgac cccgatgctc gaaactagtt tgacccctac acgcgcccgt
      841 ggattgactt gctttaagtc gtatatcaac tgggctatat cctcgatgct atagatgtcg
      901 tgatgcggtg ggggtgagat cagcgctaca tacggcgctg tgtgacgcag acgtgcaatg
      961 aagggcgtca ctttctttgg tggtagctgc ccaccctcgc cgggttttga tccctgcgcc
     1021 atcttgattt ctaattcttc ggcgcgtact aaatactccg tggtgacgcc aaaacgtgca
     1081 gaagcgactt gcttaatctt gctgccgagc gggtagccgt tcagttgctc atggtaccag
     1141 tcagggttct cgccgccttc accggtgttg ttgcgcccgc caatgctgtt catagcggcg
     1201 gcaatagtgc gatgcgtctc aggacttagc gcgccaagag acatcgcgga gatgacaaag
     1261 cgcgagcgaa tcgactccat cgactcaacc tgctctagag gaataggcgt ggtcggcacg
     1321 aaggagagaa gatcacgtaa agcagtcggc ggacgctggt agaccatttg cgtaaattgc
     1381 cggtagtcat ccatgttgcc ggtctgggct gccttttgca aagcacgtat gagtaggggg
     1441 tgataggcat gatactcgcc gctgcggcgg aaacgatagc gtccaaagtc gatcaatttg
     1501 cgtcgtcgtc cattggatac tactggcggg gacgcttcct cctgcaaggc ctggcagcct
     1561 tcgattactt gctgagcaat gtgtgcacag gtgacactgc cggggtgagc aactgaacct
     1621 gcgaaacagc gttcgatcag ctgcggttcg aggccaaccg cttcgaattg tcccgcccca
     1681 acgatgttac ggagcgtaga gatgcccata cgagccatga ctttgcgcag accaccctca
     1741 attacatgca aatagcgctc tgccgcctcc tcccgcgtca ggtgttccag atgccgctct
     1801 cccgccatgg cacgcacgct ggcaagcgcc aaagttggga cgaccgcctc tgcaccatag
     1861 ccaaggagta aggcgatctg gtgaacgtcc cagacagagc cggtttcgca gatcaaggag
     1921 atttgcgtgc gctgtccgtg tcgaataaga gcctggtgga cagcaccgac ggcaaggagc
     1981 ataggcacag gaggctgcgc tgggctggcg ctctggtcat taagaactag cagcgcaatg
     2041 ccctgagcga cagccgctac agcttcgtgc tccagccgat caagagcagc ttccagcgcg
     2101 tctggcccgc cggcgctctt gaatgtgaca tcgagcgttt gcatggggaa atctttcagg
     2161 ttgcgcatcg tttccaattg agattcgttg agaagaggag tctccaggtg cagcaatttc
     2221 gcatggagtg ctgtctcagt aagcatgctc ttgcgggggc caaggtagca gtcaagcgac
     2281 atgacgatct gttcgcgcaa agggtcg
//
LOCUS       DASZAK010000440         1533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_116765, whole genome shotgun sequence.
ACCESSION   DASZAK010000440 DASZAK010000000
VERSION     DASZAK010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116765"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VGT44_07675"
     CDS             <1..879
                     /locus_tag="VGT44_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580717.1"
                     /translation="HIDASQALVPFGGLSYSVQPAESPSLGAEPNNGTEYFLSALQFG
                     PAPLDNRIATWALTNTASLNVATPSVVLSFVVITSETYGQPPNATQKTSGPRPLATFI
                     QQSTGTRPPVELLASNDDRMNQVVFATGLLWSGVNTIVQNGTSAPRVATAYFIVKPSW
                     TTDGKLTASIAKQGYVAIDGANVLFPSIGVTASGQGVISFSVSGKNFFPSAAYALVDA
                     VNGAGRIHIAAAGQLPDDGFSGYAAYGGAGVGRWGDYTAAVADGNNIWFAAEYIPNLP
                     RTLLANWGTFVSKLHV"
     gene            1194..>1533
                     /locus_tag="VGT44_07680"
     CDS             1194..>1533
                     /locus_tag="VGT44_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580718.1"
                     /translation="MRFTSRSTLSILFLLAALLTLVSGLTYYGVSNRNTARAASATAS
                     YSGTKGSMHVVGTVKPTNLPGAKNPAQLSRVDLLKHAADARAHSVRAGSLLKNAAQPR
                     AKSSTSGASRL"
BASE COUNT          297 a          462 c          439 g          335 t
ORIGIN      
        1 catatcgacg cctcgcaggc cctggtgcca ttcggcggcc tgtcgtactc ggtgcaaccg
       61 gcggaatcgc ccagcctggg cgcagagccg aacaacggga ctgagtactt cttgagcgcg
      121 cttcagttcg gacccgcgcc tctggacaac cgtattgcca cctgggcatt gacgaacaca
      181 gcctcgctca acgtggcgac gccaagtgtg gtgctgtcct tcgtggtaat cacgagcgaa
      241 acctatggtc agccgcccaa tgccacgcag aagactagtg gtcccaggcc gctcgctact
      301 ttcattcagc aatcgaccgg cacacgtcct ccggtggagc tgctggccag caatgacgac
      361 cgcatgaacc aggttgtttt cgccaccgga ctgctgtggt ctggcgtgaa caccatcgtg
      421 cagaatggca ccagcgctcc ccgcgtcgcc actgcctact tcattgtgaa accatcgtgg
      481 actactgatg gcaaacttac cgctagcata gccaagcagg gctatgttgc catcgatggc
      541 gcgaacgtcc tgttcccctc gatcggtgtc accgctagcg gtcagggcgt gatctcattc
      601 agcgtgtccg ggaagaactt cttcccaagc gcggcttatg ccctggttga cgcggtgaac
      661 ggcgcaggcc gtattcacat cgccgccgcg ggccagttgc cggatgacgg tttctcaggg
      721 tacgctgcct atggcggcgc cggcgttggt cgctggggcg actacacggc agcggtggcc
      781 gatggcaaca acatctggtt tgccgccgag tacattccga acctccctcg cacgttactc
      841 gctaactggg gaacgttcgt cagtaaattg catgtctaac gatctgacac gcctggtttg
      901 aatggagaaa tgtaggggcg ccattcattg cgccctcgtc ccaggccgat gcacgacgag
      961 gtgggcacca tgaatggcgc ccctacattt cgtagtctac cagacccttt tgaacgattc
     1021 tgcgatggcg tttgcgtgac aatcgcaacc agatctcaac cccatcacaa ccgtttcttc
     1081 cttctcttcg gggagattgt tgcccataat aggcggacgg ggtgcctgtc gctcgctaca
     1141 ggacagatgc tccagtaatt gggtggttta tgtgtggaaa gtgagatttc ttcgtgcgat
     1201 ttacctctcg ctcaacgttg agcattcttt ttctcctggc tgcattgctt accctggtca
     1261 gcggattgac ctattatggc gtgagcaatc ggaataccgc gcgcgcggcc agcgcgactg
     1321 cttcttatag tggtacaaaa gggtctatgc atgtcgttgg aacggtcaag ccgaccaacc
     1381 tgccgggcgc gaaaaatccg gcgcagttga gcagggttga cctgctcaaa cacgctgcgg
     1441 atgcgcgcgc gcatagcgtc agggccggtt cgcttctcaa gaacgccgcc cagcccagag
     1501 cgaagtcctc gacctcaggg gcttccaggt tgc
//
LOCUS       DASZAK010000441         1634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_82423, whole genome shotgun sequence.
ACCESSION   DASZAK010000441 DASZAK010000000
VERSION     DASZAK010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82423"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VGT44_07685"
     CDS             complement(<1..559)
                     /locus_tag="VGT44_07685"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV2580719.1"
                     /translation="MTLFHHKPRKFTMMVGSIMLCLLFVACGGASTGGTSTPAPSITN
                     GKGCMKVGVLLPETATSARWDGVDRPALQADIPQLLPGATVDYANAQGNADTQQTQAQ
                     AALTKGDCILVIAPSDSIKAAAIVAAAKAKSVPVISYDRLIYSNDLNYYVSFDGVAVG
                     NLQGTYIAQHYQSYVTANGTKNTVMI"
     gene            complement(741..>1634)
                     /locus_tag="VGT44_07690"
     CDS             complement(741..>1634)
                     /locus_tag="VGT44_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV2580720.1"
                     /translation="ILTTEVMRRFRGSRFTIRLPKSVPPNVTRAFEALVPAVLLITLI
                     WGVEWFVSMHTFTGTDGKLQQLTLPILLMRAFQPLVFVQDSYPAALLEIILMMLLWSV
                     GIHGMNVVTAIALPFWLSTLTANATAGGHGIVTEPFFHIFAHLGGSGATWPLVIFMLR
                     SRSAQLRTVGKVALGPAVFNINEPVTFGVPMALNPLMMIPFVLVPVIIVTINYIVFAL
                     GLVHVPVVLQPFTVPVGISGFVATGGDFRGSLLQFFDLAVSAIIYYPFFKAWERILVA
                     REEAAAVQPVEKNARIPAGVR"
BASE COUNT          408 a          459 c          428 g          339 t
ORIGIN      
        1 tgatcatcac ggtgttcttg gtgccattag cggtgacata gctctggtag tgctgggcaa
       61 tgtacgtgcc ctggaggttg ccaactgcca cgccgtcgaa ggagacatag tagttgaggt
      121 cgttgctata aatcaggcgg tcataggaaa tcacaggcac actcttcgcc tttgccgccg
      181 ccacgatcgc tgccgcctta atactgtcgg atggggcaat caccaggata caatcgccct
      241 tcgtcagagc tgcctgagcc tgggtctgct gtgtatcagc attcccctgg gcattggcat
      301 agtcaacagt cgcgccgggg agcagctggg gaatatccgc ctgcagggct ggcctgtcta
      361 cgccatccca gcgcgctgag gtagcggtct caggcaagag tacgcctacc ttcatacacc
      421 ccttgccatt ggtgatactg ggtgcagggg tactggtacc gcctgtactg gcgccgccac
      481 aagccacgaa tagcaggcat aacatgatcg agcctaccat catggtaaac tttctgggct
      541 tgtgatgaaa taatgtcatc ttgaatctgt cctttcacat tgcacctttc ctaccaggtt
      601 tctccactcg taaacccata aggtgcctat ttcacgaacg taactgacac atgaacaata
      661 caaaggtttg tcctgtaatt cgtactctac gcgaacctcc tttccttttc gatggcatcc
      721 cgccagtagt gcgaggagcg ctagcgaaca cctgccggta tccgcgcgtt tttctccacg
      781 ggttggacag cagcggcctc ttcacgcgct acgagaatgc gctcccaggc cttgaaaaat
      841 ggatagtaga tgatagcgga gacagcgaga tcgaagaact gcagcaggct tcctctgaag
      901 tcgcctccag ttgcgacaaa tccgctgatt ccaacgggaa ctgtaaacgg ctgcaagacc
      961 acgggtacat gtaccaggcc aagagcgaac acaatgtagt taatcgtgac aatgatgacc
     1021 ggtaccagca caaaaggaat catcatgagt gggttgagcg ccataggaac gccaaacgtc
     1081 accggttcat taatattgaa taccgcaggc cccagggcca cctttcccac agtgcgcaac
     1141 tgggccgaac gtgaccgcag cataaagatt accagcggcc atgtcgcacc cgagccgccg
     1201 agatgggcga aaatatggaa gaacggttcg gtcacgatgc catgcccacc agccgtcgca
     1261 ttagcagtca gggtactcaa ccagaatggg agtgcgatag ccgtcacgac attcataccg
     1321 tggattccga ccgaccagag caacatcatt aagatgatct cgagcaacgc cgccgggtaa
     1381 ctatcctgca caaacaccag cggctgaaaa gctcgcatga gcaagatagg cagcgtcaac
     1441 tgttgaagtt taccatccgt cccggtgaag gtatgcatgc tgacgaacca ctcaactccc
     1501 cagatcagtg ttatgagcaa aacagccggt accagtgctt caaatgcgcg cgtcacattc
     1561 ggcggcacac tcttgggcaa gcgaatagtg aagcgcgaac cacggaagcg gcgcatgacc
     1621 tcggtcgtca agat
//
LOCUS       DASZAK010000442         3378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_152949, whole genome shotgun sequence.
ACCESSION   DASZAK010000442 DASZAK010000000
VERSION     DASZAK010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152949"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1247)
                     /locus_tag="VGT44_07695"
     CDS             complement(111..1247)
                     /locus_tag="VGT44_07695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2580721.1"
                     /translation="MRWRASFKTSLGRKFLLSYLLVVLVGLATLLAVTFALAPTLFQS
                     QLLQVLQTHPASMTTAEASLHILGSFLGTLLVALAVAAFAAFATSLAISLFVARRITV
                     PLQHMTRVSERISAGHYAERIEVAPVHRADELGQLAASINALAVALEEMERRRLAVIG
                     DVAHELRTPLATLEGYLEGVLDDVIEPTPQTWAMLHTQAGRLRRLVDDLQEVSRAEAR
                     QIPLSPQAVAPQRLVQSALEPLEGQFAEKGLDLQVRLPEDLPHVLADPARAVQILTNL
                     LVNALRYTPAPGRVEVMVSREEDGVAFRISDTGVGLTAEQLAHIFERFYRVDKSRARA
                     FGGSGIGLTISQSLAQAMDGDIQVESAGLGQGSSFILTLPQVHV"
     gene            complement(1252..1962)
                     /locus_tag="VGT44_07700"
     CDS             complement(1252..1962)
                     /locus_tag="VGT44_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580722.1"
                     /translation="MTRILVVDDESALVDLVRSYLEREQYEVLTASDGRTALDLVRAV
                     QPDLVVLDVMLPELDGMEVCRQLRQFSDAYVIMLTARAEEIDKLLGLTVGADDYLTKP
                     FSPRELVARIKALLRRPRQVAGAHPTSTPDDAPTPQRWEDLVIDEAQHEVTLHGQPVE
                     LTAREFALLLELARHPGRVYTRAQLLERVWGDAYYDEHVVDVHVANLRKKLEADLAHP
                     QYIETVRGVGYRFRRKRE"
     gene            2289..2957
                     /locus_tag="VGT44_07705"
     CDS             2289..2957
                     /locus_tag="VGT44_07705"
                     /inference="COORDINATES: protein motif:HMM:NF025016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEV2580723.1"
                     /translation="MIEQADEAQLDSFKSRVEEHGLARSLLESFAYIKPFGGMFGKIV
                     PYYAESYWFAMGGLTFLMFVFLTGSGIILALLGPYWWLTNPVGGFLKSFHYWSAQGFF
                     FFALLHLFRVWATGSFRGRRIFNWWIGLMIFMLAMGENLFGLLARGDWESQFVSMHSD
                     NMLFIQPFFFNLFSPGNFTADLTIHIAVIPVVIFALILAHIVLIRIMGLSKPLPVQHQ
                     QKEQ"
     gene            2962..>3378
                     /locus_tag="VGT44_07710"
     CDS             2962..>3378
                     /locus_tag="VGT44_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580724.1"
                     /translation="MESAEQVQHVATSTSPTPSGDRPPERLVRHVFWKYPLLSLVLLV
                     VVAVVAATVGSPNWPRDSVESVSKADPGGSILAFTQELDGTSSSASNAQEFGMGAPGD
                     VFVHFPLHQAGWFLGPPVQNALATWDAVSPQQRQTWA"
BASE COUNT          648 a         1039 c         1005 g          686 t
ORIGIN      
        1 gcgacggtcg ccttcgtctt cccctgcatc tgatgtgagt ataagagatg tacttcaagg
       61 gcagatgcgc gttttcatca agattcgatc aagaacatga tggtgcgaga tcatacatga
      121 acttgaggaa gcgtgagaat aaagctgctc ccctgcccaa gtccagcgct ttccacctgg
      181 atatccccgt ccatggcctg ggccagcgac tgtgagatgg tcaggccgat gccggagccg
      241 ccaaaagcgc gcgcacgtga cttgtcgacg cggtagaagc gctcgaagat gtgcgcgagc
      301 tgctccgccg taagacccac gccggtatcg ctaatccgga aggccacccc gtcctcttcg
      361 cggctgacca tcacctccac gcggccagga gcgggtgtgt agcgcagcgc gttgaccagc
      421 aggttggtga ggatttgcac ggcgcgggca ggatcggcca gcacgtgagg aaggtcctcc
      481 ggtaacctga cctggagatc cagccctttc tcggcaaact gcccttccag cggctcaagc
      541 gcgctctgga cgaggcgttg tggggcgact gcctgcggag agaggggaat ctggcgcgcc
      601 tcggcccgcg aaacttcctg cagatcgtcc accaggcggc gcaggcgacc cgcctgggta
      661 tgcagcatcg cccaggtctg cggggttggc tcgatcacgt cgtcaagcac gccttcgaga
      721 tagccttcga gcgtggcaag cggggtgcgc agttcgtgcg ccacgtcgcc aatgaccgcc
      781 aggcgacggc gctccatctc ttcgagcgcc accgccaggg cattgatgct ggcagccagt
      841 tgtccaagct cgtcggcgcg atgcactggc gccacctcga tgcgctcggc gtagtgaccc
      901 gcgctgatgc gctcggagac gcgcgtcatg tgctggaggg ggacggtgat acgccgcgct
      961 acaaagaggc tgatggccag ggaggtcgca aaggcagcga aggcggccac cgccagggcc
     1021 acgagcaggg tgccgaggaa gctccccagg atgtgcaggc tcgcctcggc ggtcgtcatg
     1081 ctggctggat gagtctggag tacttgcaga agctggctct gaaacagggt cggcgccagg
     1141 gcaaaggtga ccgccagcag cgtcgccaaa ccgaccagca cgaccagcag gtaagatagc
     1201 aagaatttcc taccaagcga ggttttgaac gatgctctcc aacgcatagg gctactcccg
     1261 cttgcgacgg aagcgatagc cgacgccgcg caccgtctcg atgtactggg gatgtgccag
     1321 gtccgcttcc agcttcttgc gcaggttcgc gacatgcaca tccacgacgt gttcatcata
     1381 gtaggcatca ccccacaccc gctcaagcaa ctgagcacgc gtataaaccc ggccgggatg
     1441 ccgtgccagt tccagaagca gggcgaactc gcgcgcggtc agttcgactg gctgcccgtg
     1501 cagcgtcacc tcgtgctgcg cctcgtcaat cacgagatcc tcccagcgct gcggcgtggg
     1561 ggcgtcatcg ggcgtactcg ttgggtgcgc gcccgcaacc tggcgaggac gccgcaagag
     1621 cgccttgatg cgcgccacca gctcgcgcgg gctgaacggc ttggtgaggt agtcgtccgc
     1681 gccaacggtc aggccgagga gtttgtcgat ctcttcagcc cgcgccgtga gcatgatgac
     1741 ataggcatca gaaaactggc ggagctggcg gcagacttcc atgccgtcca gttcgggcag
     1801 catcacgtcg agtacgacga gatcaggttg gacagcgcgc acgagatcaa gcgccgtgcg
     1861 tccatccgaa gccgtcagca cctcgtactg ctcgcgttcg agatagctgc gcaccagatc
     1921 gacgagcgct gactcatcgt ctaccactaa aattcgcgtc acaaccgtct ggctccttta
     1981 ctatgatttc gttatctacg tcaatactaa gtcgaggaga tcacagtcct ctcacgagct
     2041 tttctcacct gctcacaatg gcatcacaac ccgtgcagga gagctgattg tttcccactt
     2101 ctacaaacac ttcgattcgt gacttcctcg tgatatgcat ggttattcat gtgatgcatt
     2161 tgtgattatt ttcatgaata cttctcatat tgaacagtaa ttgactgatt gtgattttgt
     2221 tgattcgtgt gtttgctgaa gatgcctgcc tgttgcacga ggcaggtgaa tacaaggagg
     2281 aagctcacat gatcgaacaa gccgacgagg cgcagctcga cagcttcaaa agccgtgtgg
     2341 aggaacacgg cctggcgcgg tccctcttag agtcgtttgc gtatattaaa ccctttggag
     2401 gtatgttcgg caaaatcgta ccctactatg ccgagtcgta ctggtttgcc atgggcggac
     2461 tcacgttcct gatgttcgtc ttcctgaccg gcagcggcat cattctcgcg ctgctcggcc
     2521 cctactggtg gctgaccaac cccgtcgggg gatttctcaa gtcgttccac tactggagcg
     2581 cgcaggggtt cttcttcttc gcgctgctgc acctgttccg agtctgggcc accggctcct
     2641 tccgggggcg gcgcatcttc aactggtgga tcgggctgat gatcttcatg ttagccatgg
     2701 gcgagaacct cttcggactg ctggcgcgcg gcgactggga atcgcagttc gtctccatgc
     2761 actccgacaa catgttgttc atccagccgt tctttttcaa cctgttcagt ccaggcaatt
     2821 tcaccgccga tttgacgatt cacattgctg tcattccggt cgtcatcttc gcgctaatcc
     2881 tggcgcacat cgtgctaatc cgcatcatgg ggctgagcaa gccgctcccg gttcagcatc
     2941 agcagaagga gcagtagcat catggaaagc gcagagcaag tccaacacgt ggcgacaagc
     3001 acctcgccaa ccccatccgg tgaccggcca cccgagcgcc tggtgcggca cgtcttctgg
     3061 aagtatccgc tcctctcgct ggtgttactg gtggtcgtgg ccgtggtagc agcaaccgtc
     3121 ggatcgccca actggccgcg cgattcggtt gaaagcgtct cgaaggccga tccgggcggt
     3181 tccatcctgg ccttcaccca ggaactggac ggcacctcga gttccgcctc caatgcccag
     3241 gagttcggca tgggcgctcc cggcgatgtc ttcgtccact tcccgctcca ccaggccggc
     3301 tggtttctcg gacccccggt gcagaacgcc ctggccacct gggacgcagt cagcccccag
     3361 cagcgccaga cctgggcc
//
LOCUS       DASZAK010000443         1898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_543001, whole genome shotgun sequence.
ACCESSION   DASZAK010000443 DASZAK010000000
VERSION     DASZAK010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543001"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VGT44_07715"
     CDS             <1..1396
                     /locus_tag="VGT44_07715"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2580725.1"
                     /translation="YTRLPFGKTHVNKSVYYQGGTLVLRDVDAQDRPPAPFKLIKERW
                     RCEGYHYGSIQPYLQEQAVRDSVPRWQTLNLKLQEMREPHDYQVAALSAWDEAERRGS
                     IVLPTGAGKTFIAVQAISRVNRSSVVVAPTIDLLHQWYARLVNAFASDVGVYYGGEKN
                     VLPLTVTTYHSAGDLMAEYGNAFKLVIFDEVHHLPAPNWGEAALMAPAPFRLGLTATY
                     PEAHEQTNGRWRVDDLIGPIVYTKRIEDLLGQQLAHYRTQRLRVDLTEEERACYEADY
                     AIYMGFVRSRQLPRKFGPSWLMHLMRLSASDAEARRALLARQRLVQLLGRCEGKFAAL
                     DRLLLECASEQALIFTEHNTAVYEIARRHLVPAITHETNAAERKHILDGFQSGRYRVI
                     VTSRVLNEGVDVPEAKVAIVLGGTSGSREYIQRLGRVLRKVENKQAVLFEVIARKTID
                     EGKAQRRRRRREAD"
     gene            1396..>1898
                     /locus_tag="VGT44_07720"
     CDS             1396..>1898
                     /locus_tag="VGT44_07720"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEV2580726.1"
                     /translation="MLTADLVRPKLHVRGSELSIDLLNVGDARWLQTAAELIALFQGH
                     AGQSLAVWEKALESYIGERIDYVEVRGLAKVLTDAATFTPLSTPLPPVLLRERLFAHG
                     PVFSAPQLFHQQARYEVVQNAAAEMEISPEQLESAMFADRPANYQLSDAGPPWTPDGL
                     IARYNLEL"
BASE COUNT          362 a          586 c          606 g          344 t
ORIGIN      
        1 ttatactcgg ttgccattcg gaaagacgca tgtgaacaag tcggtctact atcaaggcgg
       61 tacgcttgtc ctgcgtgacg tggacgcgca ggatcgacct cccgcgccat tcaagcttat
      121 taaggagcga tggcgttgcg aggggtatca ttatgggtcg atccagccgt acttgcagga
      181 acaggctgtg cgggatagtg tgccgcgctg gcagacgctc aacctcaagt tgcaggagat
      241 gcgggagccg cacgactacc aggtggcggc gctgtcggcc tgggacgagg cggaacggcg
      301 cggcagcatc gtcttgccga cgggcgccgg gaaaaccttc atcgcggtgc aggccatcag
      361 ccgcgtcaat cgctcgagcg tcgtcgttgc gcccacgatt gatctgctgc accagtggta
      421 cgcgcggctg gtgaatgcct ttgccagcga tgttggcgtc tattatggcg gcgaaaagaa
      481 cgtgctgccg ctgaccgtca cgacctatca ctcggcgggc gacctgatgg cggaatacgg
      541 caacgccttc aaactggtta ttttcgacga ggtgcatcac ctgcccgcgc caaactgggg
      601 agaggccgcg ctgatggccc cggctccttt tcggctgggg ctgacggcca cctatcccga
      661 agcgcacgag cagaccaacg ggcgctggcg cgtcgatgat ctgatcgggc caatcgtcta
      721 caccaagcgc atcgaagacc tgcttgggca gcagctcgca cactaccgca cgcagcgcct
      781 gcgcgttgac ctcacggagg aggagcgcgc ttgctacgag gcagactacg ccatctacat
      841 gggcttcgtg cgttcccggc agctcccgcg caagtttggt ccatcctggc tgatgcattt
      901 gatgcgcctc tccgccagcg atgcggaggc gcgccgcgcc ctgctggctc gccagcgcct
      961 cgtacaactg ctcggcaggt gtgagggaaa atttgccgcg ctcgaccggc tcttgctgga
     1021 atgcgccagt gagcaggcgc tgatcttcac cgagcataat acggcggtgt acgagatcgc
     1081 tcgccgccat ctcgttccgg ccatcacgca cgagaccaac gcagccgagc gcaagcatat
     1141 ccttgatggc tttcagagcg gacgctaccg cgtcatcgtc acgtccaggg tgctgaacga
     1201 gggcgtcgat gtgccggagg ccaaggtcgc tatcgtgcta ggcggcacgt cggggtcgcg
     1261 cgaatatatc cagcgcctgg gacgagtgct gcgcaaagtc gagaacaagc aggccgtgct
     1321 cttcgaggtg atcgcgcgca agaccatcga tgaaggcaag gcgcagcggc ggcgcaggcg
     1381 acgggaggcg gattagtgct aacggctgac ctcgtcaggc caaaactgca tgtgcgcggt
     1441 tcagaactct ccatcgacct gctgaacgtt ggcgatgcgc gctggctgca aactgccgcc
     1501 gaactgatcg ccctgttcca gggtcacgcg ggtcaatcgc tggcggtctg ggagaaggct
     1561 ctggaaagct acataggcga gcgcatcgac tatgtggagg tacgcgggct ggcaaaagta
     1621 ctgacagacg cggcaacctt cacgcctctg tcaacgccgc tgcccccggt gctgctgcgc
     1681 gagcggctct tcgcgcacgg ccccgtcttt tccgcgcccc agctttttca ccagcaggcg
     1741 cgctacgagg tggtacaaaa cgcggcagcc gaaatggaga tttcccctga gcaactcgaa
     1801 tcagccatgt tcgcggatcg acccgccaac tatcaactca gcgatgccgg gccgccgtgg
     1861 acgcccgatg gcctgatcgc gcgctacaac ctggaact
//
LOCUS       DASZAK010000444         4703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_330173, whole genome shotgun sequence.
ACCESSION   DASZAK010000444 DASZAK010000000
VERSION     DASZAK010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330173"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1131)
                     /locus_tag="VGT44_07725"
     CDS             complement(130..1131)
                     /locus_tag="VGT44_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV2580727.1"
                     /translation="MAQETQKAVSSTTLVDVKGLKVYFPIKGGILNRTVGQVKAVDGV
                     DLFVRRGETLGLVGESGCGKSTTGRAILQLIKPTAGSVVLDGVELTKLSPGQVRAKRD
                     TMQMIFQDPYGSLNPRFTIGQIVSEPLENFKRGNKKEIQDEVAHLLEVVGLNPFFMNR
                     FAHEFSGGQRQRIGIARALALHPSFIVADEPVSALDVSIQAQVLNLLEDLQSQFGLTY
                     LFVAHNLSVVKHISDRVAVMYLGRVAELADSEDLYETPLHPYTQALLSAIPVPDPEKE
                     SKRKRIILEGDVPSPVNPPPGCNFNPRCWKAQEICREVIPPLEEKQPNHFAACHFPG"
     gene            complement(1145..2122)
                     /locus_tag="VGT44_07730"
     CDS             complement(1145..2122)
                     /locus_tag="VGT44_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580728.1"
                     /translation="MTENLLDVKNLKTYFFTRGGVVKAVDDVSFGIKPGQTLGVVGES
                     GCGKSVTALSVMRLVATPPGKIVGGEINYNGENILEKNRDEMTDLRGSQISMIFQDPM
                     TSLNPVFTVGYQIQETVVRHRKGVSKDQAWKRAIEMLDLVRIPDAKRRVKNYPHEFSG
                     GMRQRVMIAIALACNPKLLIADEPTTALDVTIQAQILELMKGLSKEFDTAVMLITHDL
                     GVVAGTCEFVNVMYAGHVVESAPVNQIFETPAHPYTVGLLHSIPRLDDERGVRLNPIA
                     GQPPDLLNPPTGCPYAPRCPKAQSRCLKERPELMSVGRGEQVAACFYPY"
     gene            complement(2206..3321)
                     /locus_tag="VGT44_07735"
     CDS             complement(2206..3321)
                     /locus_tag="VGT44_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2580729.1"
                     /translation="MDTKDKDFELASPGVMMPDTTMSPLAMETASENGLLQIVEPETS
                     VVTPEGKPQKSQTPLQDSLRRLRKDKRAMVSLGVIILFLLIPIFGPPIYQHIGGIYQS
                     PINGNIGPDKYHNPFWTELTNQDLLPNSMYWLGTDGIGRDQMARLMQGMLVSISIALL
                     VEVFDILLGVSVGVLAGYFGGWIDQLLARFTDVMFAFPGLLFVIMVAGIFGSGADVAF
                     SKIPIIGANGNARLLLVGLAIAITAWPLMARYVRGQTLQLKEQQFIEAARTSGTTNAK
                     IILRHIIPNLFSIVVVAATLDISGTIIGEAGLSFLGLGVQPPGSSVGLMISDAQGSLG
                     VHPFEVLLPTVVLTIIVLAFSFLGDGLRDAFDPRSKD"
     gene            complement(3334..4293)
                     /locus_tag="VGT44_07740"
     CDS             complement(3334..4293)
                     /locus_tag="VGT44_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2580730.1"
                     /translation="MVQFLVKRFIGLIFVVIAVTFVTFVLGLNAPGDPIRDMLGTHFN
                     QQVYDSLRHAYGLDLPWYQQYWNFLTNLFRFNFGYSFHQQNYPVWYILRDGVPVSTEL
                     AFWALLIQLLVGIPIGIISALKANTWVDTANMGFMLILYALPVFVLAVFAQLVIVWFD
                     TNTGNTWPVAGWGNNWSYSLSDIQIKIIPILVFAASGTALYARIARTSMLEVLRQDYV
                     RTARAKGLRESIVVYRHAFRNAMIPLITIFGLSLGLLVTGAFFIEQIFNIPGIGQATV
                     SSISYRDYPVIQATTVLLAVAVVVGNLISDLLYSVADPRIKNE"
BASE COUNT          990 a         1265 c         1421 g         1027 t
ORIGIN      
        1 taagggcggg tgcggtgggg agtggggtgg agaccctcgc ggtcgccctc tcgtccactc
       61 tactctggtg gccaggatat tttgtcaatc gccattaccg gctcctacgg ttttagttgc
      121 tttcagctcc tacccaggga aatgacaggc ggcaaagtgg ttgggttgct tctcttcgag
      181 aggcggaatg acttcgcggc agatttcctg ggctttccag cagcgcgggt taaagttgca
      241 gccgggcggt gggttcaccg gactgggaac gtcaccctcc aggatgatgc gtttgcgctt
      301 cgactccttt tccgggtcgg gaacggggat tgccgacagc agcgcctgcg tgtagggatg
      361 caagggcgtc tcgtataaat cctcgctatc tgccagttca gccacgcgtc ccaggtacat
      421 gacggcgacg cggtcgctga tgtgtttgac caccgagagg ttgtgcgcca cgaagagata
      481 ggtcaggcca aactgcgact gcaaatcctc caacaggttg agcacctgcg cttgaattga
      541 gacgtccagg gctgataccg gttcgtccgc cacgatgaag gaagggtgca gagccagggc
      601 gcgcgcgatg ccgatacgtt ggcgctggcc accgctgaac tcgtgcgcga aacggttcat
      661 gaagaaggga ttcaggccga caacctcgag caggtgcgcg acctcatcct gaatttcttt
      721 tttattgccc cgcttaaaat tctccaacgg ttccgagacg atctgcccga tggtgaagcg
      781 gggattcaac gagccatagg ggtcctggaa gatcatctgc atcgtatcgc gttttgcgcg
      841 aacctggccg ggagagagtt tcgtcagctc gactccatcg agtaccacgg aaccagccgt
      901 cggcttgatt aattgcaaga tggcgcgacc ggtcgtggat ttcccacagc cgctttctcc
      961 caccagtccc agcgtttcgc cgcgacgaac gaagaggtca acgccgtcaa ccgcttttac
     1021 ctgcccgacg gtgcgattta agatgccccc ttttatgggg aaatagacct tcagaccttt
     1081 tacgtcaacc agcgtggtgc tgctgaccgc tttttgcgtt tcttgtgcca tgctgcttgt
     1141 ccccttagta tggatagaag caggccgcaa cttgttcgcc gcgacctacg gacatgagtt
     1201 ctggtcgctc tttcaggcag cgactctgcg cctttgggca acgtggagca taggggcaac
     1261 ccgtgggcgg gttgagcagg tcgggcggtt gaccggcaat gggattcagt ctgacgccgc
     1321 gctcatcgtc caggcgagga atagagtgga gcagaccgac tgtgtaggga tgcgcgggtg
     1381 tctcaaagat ctggttgacc ggcgcggatt ccacgacatg cccggcatac atgacattca
     1441 caaactcgca ggtaccggct acaacaccca ggtcatgcgt aatcaacata accgccgtat
     1501 caaactcctt cgatagaccc ttcatcagtt ccagaatctg tgcctgaatg gtcacatcaa
     1561 gcgcggtcgt cggctcgtcg gcgatcagca gcttgggatt acaggccagg gcaatggcga
     1621 tcatgacgcg ctggcgcatg cctccgctga attcgtgcgg atagtttttc acgcggcgct
     1681 tcgcatcggg gatgcgcacc aggtcgagca tttcgatggc gcgtttccac gcctggtctt
     1741 tgctgacgcc tttgcggtgt cgcaccaccg tctcctggat ctggtagccg acggtaaaca
     1801 cggggttgag ggaggtcatg gggtcctgga agatcattga aatctggctg ccgcgcaggt
     1861 cggtcatttc atcgcggttc ttctccagga tgttttcgcc gttgtagttg atctcgcctc
     1921 ccacgatttt tccgggaggt gtggcgacca ggcgcatgac ggagagcgcg gtcacgcttt
     1981 tgccgcagcc gctttcaccg actacgccca gtgtttgccc cggtttgatg ccgaatgaca
     2041 catcatcgac ggctttcacc accccgccgc gtgtgaaaaa gtaggtcttg aggtttttaa
     2101 catcaagcaa attctctgtc attgtcgtca ctccgctcac ttgtctctaa ccagcctgcc
     2161 aacgcactgt ctgctatggc ttagccctca ggcaggctgc aaggactaat ctttagatcg
     2221 aggatcaaag gcatcacgca gcccgtcgcc caggaaggag aaggctagca cgatgatcgt
     2281 cagaacaacg gtgggcagca gcacctcgaa cggatgcacc ccgaggctgc cctgcgcgtc
     2341 cgagatcatc aagccaacgc tggagccagg gggctgtacg cccaggccga gaaagctcag
     2401 cccggcctcg ccgatgatgg tgccggaaat gtccagcgtg gcagccacga cgacgatgct
     2461 gaacaggttc gggataatgt ggcgcaagat gatcttggca ttggtggtgc cggacgtgcg
     2521 ggctgcttcg atgaactgtt gctccttcaa ttgcagcgtt tgcccgcgca cgtagcgggc
     2581 catgagaggc caggcggtga tggcgatagc caggccaacc aggaggagac gggcgttgcc
     2641 gttcgcgccg ataatgggta ttttgctgaa cgccacgtcg gcccccgatc cgaagatgcc
     2701 ggcgaccatg atgacgaaga gcaggccggg gaaggcgaac atgacatccg tgaagcgcgc
     2761 cagcaactgg tcgatccagc cgccgaagta cccggccagc acaccgacgg agacgcccag
     2821 caggatatcg aagacctcga cgagcagagc aatcgagatc gacaccagca taccctgcat
     2881 caggcgggcc atttgatcgc ggccaatgcc gtcggtgccg agccagtaca tgctgttggg
     2941 caggagatcc tggttggtga gttccgtcca gaaggggttg tggtatttat ccgggccaat
     3001 gttgccgttg atggggcttt gatagatgcc gccgatgtgt tgatagatcg gcgggccaaa
     3061 aattgggatg agcaggaaga ggataatcac ccccaggctg accatggcgc gtttgtcttt
     3121 gcgcaggcga cggagtgaat cctgcaaggg ggtttgcgat ttttggggtt ttccttcggg
     3181 tgttacaacc gaggtctcag gctcaacgat ttgtagcaag ccattttccg atgccgtttc
     3241 catagcgaga ggcgacatgg tggtgtcggg catcattacc ccaggagagg ccagttcaaa
     3301 gtctttatct ttcgtatcca tgagacgtgc ctcctattcg ttcttgatgc gcgggtctgc
     3361 tacactatac aggagatcgg agatcagatt gcccacgacg acggcaacgg cgagcagcac
     3421 ggtggtggcc tggatcaccg gatagtcacg gtagctgatc gatgagacgg tcgcctgccc
     3481 gatgccgggt atgttgaaga tttgctcgat gaagaaggcg ccggtgacca gcaagcccag
     3541 tgagaggccg aaaatcgtga tgagcgggat catggcattg cggaaggcat ggcgatagac
     3601 cacaatgctc tcgcgcaagc ccttggcgcg ggccgtgcgc acataatcct ggcgcagcac
     3661 ttcaagcata ctggtgcgcg ctattctggc gtacagggcg gtgccgctcg cggcaaacac
     3721 cagaatcggg atgattttga tctggatatc gctgagcgag taggaccagt tattacccca
     3781 gcctgctacc ggccaggtat taccggtatt cgtgtcgaac cagacgatca ccagttgcgc
     3841 gaagacggcc agcacaaaga ccggcagggc gtagaggatc agcatgaaac ccatgttggc
     3901 ggtatcaacc caggtattcg ctttgagcgc agaaataatc ccgatcggga tgccgaccag
     3961 cagttgaatg agcagcgccc agaaggccag ttccgtcgaa acggggacgc cgtctctcaa
     4021 aatgtaccag acgggatagt tctgctggtg gaaggaatag ccgaagttga accggaacaa
     4081 gttggtaaga aaattccaat actgctgata ccagggaagg tcgaggccat aggcgtgcct
     4141 gagactgtcg tagacctgct ggttgaaatg tgtccccaac atgtcgcgaa tggggtctcc
     4201 tggggcattc aggcccagga caaaggtgac gaaggtcaca gcgatgacca cgaagatcaa
     4261 gccgatgaac ctcttgacca ggaattgaac catactgtac cctctactct cttgtgtgcg
     4321 cttgcagcga tgtaagagaa tacttcgatt gttttctctt agcttttagc tcatggtaca
     4381 ttgctcatac tctatgcctt ctcatggaga gaacgcttac gcaatggcac ttttccgtat
     4441 agtgcgttct cttctattgt gaaggtggtc ctacggggat ggaacgtaat cttattgtat
     4501 gtgagacgcc tgtgcttgtc aagcattttc gcgcctcacg aaaagcgctg taaattattg
     4561 ctgaattgct cccgcagtat tgctggattg gctcgccacg actattgccg ttatgttgta
     4621 gggacagggt gcggagcggc gggggccttg tgcctgtccg ggtggcaagc gcggatcgtg
     4681 acctccaacg cggacaggcc ccc
//
LOCUS       DASZAK010000445         6031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_579222, whole genome shotgun sequence.
ACCESSION   DASZAK010000445 DASZAK010000000
VERSION     DASZAK010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579222"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2029)
                     /locus_tag="VGT44_07745"
     CDS             complement(<1..2029)
                     /locus_tag="VGT44_07745"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HEV2580731.1"
                     /translation="MIFMGKRSTAHTDRKKEDRKPRIEDQFADKPTAQADSDRLDFKR
                     IVSFLADFISDDDKRRTITTIGIYGEWGSGKTSFLKMLDERLRNEKQIYPIWFYAWRY
                     DKQDDLWAALLQSILNQAGKLKGQNLLRKWWIKFRIWFRYKSLGEGTWEVTRKFMLGG
                     LRIFVILIILFVLFYITFGVGGPSIATFLQMIPFLKTVPSNVLILIVNAFLVFLGAAT
                     ADVGKIRDLLVGGIQVDFTKFERKSNFRDRISLLDEFSRELQDLIDLLRPPKKPLVVI
                     IDDLDRCLPESALRVLESIKVFLENSNCIFLLGLDKQVLERAVAERYKESSSTDHNQP
                     DTRSGMDAHQRKYHLEYFDKIIPLAIAVPQMLQTQNAIKDFITKLNSGIDKEVEEYAD
                     IFGFSLSTNPRKIKRTLSSFRFVRDLVNARIEAQEASNAEKNIRFSLLAKLITIQSQF
                     PDLYESAIREPSLLEDLETHFRNPDLQTSEKAAYAESYANKYQGLREILIRGKNKDKE
                     DSFVGQSIHNYAFQIGTLAEVGLSVRKQSLQNEIFWKIPGRNELFTAREVILHSLRDT
                     FEQRLESHNACIALSGSYGIGKTQIAIEYAHRYRQDYKAVFWVHAETRNMMMIDYGAI
                     AEYVELPEKNEQNQNLVNEAVKTWFERQTNWLLILDGVDDLSQVNDLLPHLS"
     gene            2382..2990
                     /locus_tag="VGT44_07750"
     CDS             2382..2990
                     /locus_tag="VGT44_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580732.1"
                     /translation="MSDQDMEMQFADPDWQPASARPGRYPGEQARAVPFEIKHAPASN
                     PQADGQPATRLWTSGPGQTPPWTIQPTSADASRGTGQRQKKHQQQPAPARLKSRQQRW
                     LLGLLARVAVVVIWVTTPLVTRAFHGGWIVPLLGVLFLPVTALTYAVVFALAGGVTGL
                     GWLWIAGAVLLDLASHAGQSNKRSSGQAQHTSKDATLSTEGQ"
     gene            3141..5498
                     /locus_tag="VGT44_07755"
     CDS             3141..5498
                     /locus_tag="VGT44_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEV2580733.1"
                     /translation="MGTIDQKRLELIHRYWSAANYLTVGQIYLQANPLLKEPLRPEHI
                     KPRLLGHWGTSPGLSFIYLHLNRLIQEHDVDMIYLAGPGHGGPALLANVYLEGTYSEI
                     YPHITQDTEGMRLLFRQFSTPGGVPSHVSVPTPGSIHEGGELGYVLNHAFGAAFDNPD
                     LVVVAVVGDGEAETGPLEGAWKSIKFLNPIHDGAVLPILHLNGYKISGPTVLGRDNDA
                     DVQRLLSGHGYDVHVVEGDDPMVMHQAFAETLEICYAKIRSFQQEARTHGFTREPTWP
                     AIILRTPKGWTGPKVVDGLPIEGTFRAHQVPVADVRENAEHLRILEQWMRSYRPEDLF
                     DEQGRLKDELAVLAPRGERRMGANPHANGGKLMVKLDLPAFTDYAIDVPKPATEQHES
                     TRQLGKMLRDIFTKNADQRNFRLFCPDETNSNRLGNVFEVENRTFVEPILASDDHLSP
                     DGRVMEVLSEHLCHGWLEGYLLTGRHGLFATYESFALVSASMTVQHTKWLEEGIKLPW
                     RAPISSLNILLTSTCWRNDHNGFSHQGPGLIDTVLSKKGTVARIYLPPDANCLLSVAD
                     HCLRSRDYVNLIVIDKQPQLQWLSMPDAVDHCTRGLSTWQWASNDERVEPDVVLACAG
                     DIPTLETVAAAWWLRHHLPELKVRVVNVVDLMKLYPPLLHPHGMDDATFVEHFTQDKP
                     VVFAFHGYARAIHEVVHGRPNTPRFHVRGFMEEGTTTTPFDMVVLNHMSRYHLCIEAM
                     RRATRLRGLTPPLIAECNDLLARHTEYVRANLEDMPEIRDWTWSD"
     gene            complement(5821..>6031)
                     /locus_tag="VGT44_07760"
     CDS             complement(5821..>6031)
                     /locus_tag="VGT44_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580734.1"
                     /translation="YFHFSFLSMLFVANIAMHLPALLGISLAMMAVSLVLHGWLLQQK
                     LKLRQSPSQPSALSSETFGMKETPP"
BASE COUNT         1361 a         1701 c         1599 g         1370 t
ORIGIN      
        1 tgcttaaatg cggaagcagg tcatttacct gactcaggtc atcgacgcca tcaaggatga
       61 ggagccagtt ggtttgccgt tcaaaccagg ttttcacggc ctcgttaacg agattctgat
      121 tttgttcgtt cttttcgggc agttccacgt actcagcaat agcgccataa tcaatcatca
      181 tcatattgcg agtttcagcg tgtacccaga acaccgcctt atagtcctgg cgatagcggt
      241 gggcatattc gatggcgatt tgtgttttgc cgattccata tgatccgcta agggcaatac
      301 aggcattgtg tgactcgagt cgctgctcaa atgtatcgcg taggctgtgc agaatgacct
      361 cgcgagcggt aaacaactca tttcgaccag gaatcttcca aaagatctcg ttctgcagag
      421 attgcttgcg caccgacagt cctacctcgg ccaatgtgcc tatctggaag gcatagttgt
      481 gaatgctctg gccgacaaag ctatcttctt tgtctttgtt ctttcctctg attagaattt
      541 cgcgcagccc ctggtattta tttgcgtaac tttcggcata cgccgctttc tcggacgttt
      601 gcaggtcagg attgcgaaag tgggtctcca ggtcttctaa caatgaaggc tctctaatcg
      661 cgctctcgta gaggtctggg aactggctct ggatggtgat gagctttgca agtagggaga
      721 agcgaatgtt cttttcagcg ttcgacgcct cttgcgcttc aatacgcgcg ttgacgaggt
      781 cgcgcacaaa acggaaggag cttagggttc gtttgatctt tcgcgggttg gttgacaggg
      841 agaaaccgaa aatgtctgcg tactcctcga cctctttgtc gatgccgctg ttcagttttg
      901 tgatgaaatc cttgatagcg ttttgtgtct ggagcatctg tggcacggca atagccaggg
      961 ggataatctt gtcaaaatat tccaggtggt atttcctctg atgcgcatcc attcctgagc
     1021 gagtatctgg ctggttgtga tcggtagagg atgattcttt atatcgctcc gcgacagctc
     1081 tttccaatac ctgcttatcc aggcccaaca ggaagatgca attgctattc tccagaaaga
     1141 ctttaatcga ttcaagcact ctcagcgcgc tttctggtaa acagcgatcc agatcatcga
     1201 taataacgac caggggtttc ttgggcggac gcagcaagtc gatcaaatct tgaagttccc
     1261 ggctaaattc atccagtaga gagatacggt cgcgaaaatt gctcttccgc tcgaattttg
     1321 tgaaatcaac ctgaataccc ccaaccagca ggtcgcgtat cttccccacg tccgcggtag
     1381 ctgctcccaa aaaaaccagg aatgcgttga cgatcagaat cagcacattt gagggaacag
     1441 ttttcaagaa agggatcatt tgcaagaaag tggcgataga tggccctcca acgccgaagg
     1501 tgatgtagaa caagacgaag agaataatca agatgacaaa aatgcgcagg ccgccgagca
     1561 tgaacttgcg ggtcacttcc caggttccct caccgaggct cttatatctg aaccagatac
     1621 gaaattttat ccaccattta cgaagcaagt tttggccttt gagtttaccc gcctggttca
     1681 atatcgactg gagtaaggca gcccatagat cgtcctgttt gtcgtatcgc caggcataaa
     1741 accagatagg gtaaatttgt ttttcatttc tgagacgctc atcgagcatc ttcaaaaagc
     1801 tcgttttgcc gctgccccat tcgccataga taccgatggt tgttatagta cgtcttttat
     1861 catcatcact gatgaagtca gccaggaagc tgacgatgcg tttaaaatcc agacgatcag
     1921 aatcagcctg ggcagtaggc ttatccgcga actggtcttc aatccggggt ttcctatctt
     1981 ccttttttct atccgtatgc gctgtgcttc gcttgcccat gaatatcata gagcacacct
     2041 cctcaatctt gatttagaag attaggacac attttttaga tcagacgcgc atgaaatacg
     2101 ccaggtaaag caatccactg caatgatgct catgtatatt aacgcatgat ggagtattgg
     2161 gcaagactac gttggtcagt tttcctgctc tacctgcccc tggccctgaa ctgctgccca
     2221 attcgatcag ccatagctgc tctcctgaaa gctctcgtgc ccagcataag cgtcgtattt
     2281 tgcaaccctt gttttcgcct gttcgtacag tagataatgc gacgtgtccg tttgcttgcc
     2341 gcctcgcggt ggtcggcatt tgagagagga gtgtgccagc tatgagcgac caggacatgg
     2401 aaatgcaatt tgctgacccg gattggcagc ccgcctcagc ccggccaggc cgctatcccg
     2461 gcgaacaagc acgcgctgtt ccgtttgaaa tcaagcatgc tccagcttcg aacccgcaag
     2521 ccgatgggca gccagctacg cgtctctgga catcaggtcc gggtcagaca ccgccctgga
     2581 cgatccagcc caccagcgct gatgccagca gggggacagg tcagagacaa aagaagcatc
     2641 agcagcagcc cgcgccagcg cggctcaaga gccggcaaca gcgctggctg ctcggacttc
     2701 tggcacgcgt cgcggtggtg gtgatctggg tcacaacccc tctggtcact cgcgcctttc
     2761 acggaggctg gatcgtgccg ttgctgggcg tactcttttt accggtgaca gcactgacgt
     2821 atgccgtcgt ctttgcgctt gccggcggcg tgaccggcct gggctggctc tggatcgccg
     2881 gggctgtgct gctcgacctg gcgagccacg ctgggcagtc gaacaagcgc agcagcggtc
     2941 aagcgcagca cacctcgaaa gacgccaccc tttccacaga ggggcagtga caatccccca
     3001 tgctagagag cgaaaccagg acgcgcttta tgacgttaat attctgataa caggtttgtg
     3061 gtatcctgat gtctggaaat gactttaagg ctactgctac ctcctacagc ataaacgctt
     3121 agcgcagaat ggagcatatc atgggaacga ttgatcagaa gcggcttgag cttatacacc
     3181 gctactggag tgccgccaac tatttgacgg tcggccagat ctatttacag gcgaacccgc
     3241 tcttaaagga gccgcttcga cctgaacaca tcaagccacg cctgctggga cattggggaa
     3301 cctctcccgg tctcagcttc atttatttac acctcaaccg cctcatccag gaacacgatg
     3361 tggatatgat ctacctggcc ggaccaggac acggcggccc tgcactgctg gccaacgtct
     3421 acctggaagg cacctactcg gagatctacc cccatattac gcaggatacc gaggggatgc
     3481 gcctgctctt tcgccagttc tcgacgccgg gaggcgtgcc aagtcacgtc agcgtgccaa
     3541 cgccaggctc catccacgaa ggaggagaac tcggctacgt cctcaaccat gccttcgggg
     3601 ccgctttcga caatcccgac ctggtcgtcg tggcggtcgt tggcgacggc gaggcagaaa
     3661 cggggccgtt ggagggggcc tggaagagca tcaagttttt aaacccgatt catgatgggg
     3721 ctgtgcttcc cattttgcac ctgaatggct acaagatctc tggccctacc gtcctgggtc
     3781 gcgacaacga tgccgatgta cagcggctac tctccgggca cggctacgat gtccacgtcg
     3841 tggaaggcga cgatccgatg gtcatgcacc aggcctttgc cgagacatta gagatctgct
     3901 acgccaagat ccgctcgttt cagcaagagg cccgaacgca tggcttcacg cgcgaaccga
     3961 cctggccagc cattatcctg cgcactccca agggctggac aggaccaaaa gtggtcgatg
     4021 ggcttcccat cgagggaaca ttccgcgcgc accaggtgcc ggtagcagac gtaagggaga
     4081 acgcggaaca cctgcgcatc cttgagcagt ggatgcgcag ctaccggccc gaagacctct
     4141 tcgacgagca aggccgcctg aaggatgaat tggccgtcct cgcgccacga ggagaacgcc
     4201 gcatgggagc caacccacac gccaatgggg gcaagctcat ggtgaaactg gatttgcctg
     4261 ctttcaccga ttatgcgatt gacgttccta agcccgcgac cgagcaacac gagtcgacgc
     4321 gccagctcgg taagatgctg cgcgacatct ttacgaagaa cgccgatcag cgcaatttcc
     4381 gcctcttctg ccccgacgaa accaactcga atcgcctggg caacgtcttc gaggtcgaga
     4441 accgcacctt tgtggaacct attctcgcca gtgacgatca tctctccccc gacgggcgtg
     4501 tgatggaggt actgagcgaa cacctctgtc atggctggct ggaaggctat ctcctcaccg
     4561 ggcggcatgg cctgttcgcc acctacgaga gctttgcgtt ggtctccgca tcgatgactg
     4621 tgcagcacac caaatggctg gaagaaggca tcaagctgcc ctggcgcgcg ccgatctcct
     4681 cgctgaacat tctgctgacc tcgacctgct ggcgcaacga tcacaacggc ttcagccacc
     4741 aggggcctgg tctgatcgac accgtcctct cgaagaaagg caccgtcgcg cgcatctatc
     4801 tgccgccgga cgcgaactgt ctgctctcgg tggccgacca ctgcctgcgc agccgcgact
     4861 acgtcaacct gatcgtgatc gataagcagc cacaactgca atggctctcc atgcccgacg
     4921 cggtcgatca ctgcacccgc ggtctttcca cctggcagtg ggccagcaac gacgagcgcg
     4981 tggaaccaga cgtggtgctg gcctgcgcgg gggatattcc caccctggag acggtagccg
     5041 cggcctggtg gctgcggcac cacctgcccg aattgaaggt gcgcgtggtc aacgtcgtcg
     5101 atttgatgaa gctctacccg ccgttattgc atccgcacgg catggatgat gctaccttcg
     5161 ttgaacattt cacgcaagac aagccggtgg tctttgcctt tcacggctat gcgcgcgcca
     5221 ttcacgaggt cgtgcatgga cgccccaaca ccccgcgctt ccacgtgcgc ggatttatgg
     5281 aggaaggcac aaccaccacg ccctttgaca tggtcgtgct gaatcacatg agtcgttacc
     5341 acctctgcat cgaggccatg cgacgcgcca cccgcctgcg cggactcacc ccgccgctca
     5401 tcgccgagtg caacgacctg ctcgcccggc atacagaata cgtccgggcc aatctcgaag
     5461 acatgccgga gatacgcgac tggacctggt cggactgaat gtaacaaagg aaaccctgca
     5521 ccggcaacta ctgtagcctg gttgtccaac cccacctcta ccgcgtcgat acctgaagta
     5581 tcagcgcagt agaggacacc atccccaatg ttgtgtaccg aggaggcttt gaagagatac
     5641 gaccgaaacg agctgtttga ccatcgtttg ggccgtgatg caaggcaaca aattcgctta
     5701 ccaaagattc cggtctccta aaggctgctc aatgatggtg ctggcaaaca agctaccagc
     5761 tttgaaggct ggtacagagg atgcttttct ttgttttgtt ttcttgtgta tcattttcca
     5821 ttatgggggt gtctccttca tcccaaatgt ttcactcgac aaagcagatg gctgggaagg
     5881 agactgccgc aacttgagct tctgctgcag cagccaccca tgtaaaacga ggcttactgc
     5941 catcatcgcc agcgagatac caagcagcgc aggcaagtgc atggcgatgt tggcaacaaa
     6001 gagcatgctg agaaaggaga aatggaagta g
//
LOCUS       DASZAK010000446         3714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_437621, whole genome shotgun sequence.
ACCESSION   DASZAK010000446 DASZAK010000000
VERSION     DASZAK010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3714
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437621"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VGT44_07765"
     CDS             <1..1226
                     /locus_tag="VGT44_07765"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV2580735.1"
                     /translation="LDTSVAIRWIKEHYDLDVITVTIDVGNDRDLPAIAARGEKIGAV
                     KALVVDARADFVRYFLWPALQAGAIYEGHYPLATALARPLIARLLVEVARAEGAVAVA
                     HGCTGKGNDQVRFDVSINTLAPDLRIIAPVREWSMTRDNEIVYAAEQGIPIPVTNASP
                     YSVDQNLWGRSVECGILEDPWAEPPADVFEWTHDPASSPAEASYVEITFEHGVPTALN
                     GEESDGVTLIEYLNEQAGKYGIGRIDSIENRLVGIKSREVYEAPAATVLLAAHKALES
                     LTLSRDQARFKEIVAGEYARLIYNGQWYSALHQDLAAYVQSTQRFVSGTVRMKLSPGQ
                     CAVVGRKSEHSLYNHSLATYDTGDQFDHSAALGFIKLWGLPLTTQAQAQLLPSIGFEG
                     NLLKGAHNDERSLHP"
     gene            1223..2206
                     /locus_tag="VGT44_07770"
     CDS             1223..2206
                     /locus_tag="VGT44_07770"
                     /inference="COORDINATES: protein motif:HMM:NF019603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV2580736.1"
                     /translation="MTARIYYDNDADLSIIQGKRLAFIGYGNQGAAQSQNLRESGVSE
                     IVIGNRDDNYKKAALDAGFLVVSLAEAAAWGDAVLLLIPDEEQPSVFSEQIAPYLRPG
                     STLVVASGYNIAFNLLAIPDAVDAVMVAPRMIGAAVRDLFMKGRPYPCFVSTERDVSG
                     NALALALSIARGIGATRGGAVASSAVEEAALDLFSEQAFWPTVLLAMQSAYEVLHAAG
                     FSDEAILYEMYLSKEPAEVFERFADLGIFGQMTLHSHTSQYGQLRALLADNGAALRER
                     FAHVLHDDILSGAFAREWSDVQAKGPERLEQLRAAALTGSLAQAEKRVMKQ"
     gene            complement(2207..2491)
                     /locus_tag="VGT44_07775"
     CDS             complement(2207..2491)
                     /locus_tag="VGT44_07775"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV2580737.1"
                     /translation="MNLHTKSARSRGETEQRLYMLVAWREAPFFTERERAALAWAEAV
                     TLIAVDHVPDDVYEETRKHFTDEELVYLTLAIVSMNGWNRLAVSFRIPPE"
     gene            complement(2492..2659)
                     /locus_tag="VGT44_07780"
     CDS             complement(2492..2659)
                     /locus_tag="VGT44_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580738.1"
                     /translation="MEMQPRLEYGKVVHGIGRAMNGLDQLVKSSALADQEPLLIELVK
                     IRTSQINGCAY"
     gene            complement(2801..>3714)
                     /locus_tag="VGT44_07785"
     CDS             complement(2801..>3714)
                     /locus_tag="VGT44_07785"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV2580739.1"
                     /translation="EEGLHLPTLLELAAAKRPLSDYPGAAHWQTALDGLRAVEADALV
                     EASPTNLRDAEPALSHMRIALSNRMHVVSANKGPGALAASELFALARQNGVQLRMESA
                     VMAGTPVLSTIREGMAGARVNAIRGILNGTTNYILTGMSSGQGYADALAQAQAKGYAE
                     SDPTADVEGYDAVAKTMILAALVFNHPLKVEEVRRVGIAQISRQQVQAAADAGQRIKL
                     IASLQLHPGVDGQSASLEASVQPTALPLSDPLARVDGAMNAITIHADTLSAVTIIGPG
                     AGGIETAQGLLADVLACVLMEINQSTS"
BASE COUNT          728 a         1205 c         1088 g          693 t
ORIGIN      
        1 gcctggatac gtccgtcgcc attcgctgga ttaaagagca ttacgacctc gacgtgatta
       61 cggtcacgat cgatgtcggc aacgatcgcg acctgcccgc tatcgccgcg cgcggagaga
      121 aaatcggcgc ggtcaaagcg ctcgtcgtgg atgcgcgcgc ggatttcgtg cgctactttc
      181 tctggccagc gctgcaggcc ggggcaatct acgaggggca ttatcccctg gcgaccgcgc
      241 tggcgcgccc actgatcgcg cgcctgctgg tcgaggtggc gcgcgccgag ggagccgtcg
      301 cggtagcgca tggctgcaca ggcaagggca acgaccaggt acgcttcgat gtctcgatca
      361 acacactcgc gcccgatctg agaatcatcg cgcccgtgcg cgaatggagc atgacgcgcg
      421 ataacgagat tgtctacgcc gccgaacagg gcatccccat ccccgtcacg aatgccagcc
      481 cctactcggt cgatcagaac ctatggggtc gcagcgtcga atgtggcatc ctcgaagacc
      541 cctgggccga gccgcccgcc gacgttttcg agtggacgca cgacccggcc tcgtcgcccg
      601 ccgaggccag ttatgtcgag atcacctttg aacatggtgt ccccaccgcg ctcaatggcg
      661 aagagagcga cggcgtaacc ctgatcgaat acctcaacga gcaggccggg aaatacggca
      721 tcgggcgcat cgactctatc gaaaaccgcc tggtgggcat caagtcgcgc gaggtctacg
      781 aagccccagc cgccaccgtg ctgctcgccg cgcacaaagc cctggaaagc ctgacgctca
      841 gccgcgacca ggcgcgcttc aaggaaatcg tagcgggcga gtacgcgcgt ctgatctaca
      901 acggccagtg gtacagcgcg ctgcaccagg acttagcggc ctacgtgcaa agcacccagc
      961 gtttcgtcag cggcaccgtg cgcatgaaac tctcccccgg ccagtgcgcc gtcgtcgggc
     1021 gcaagtcgga gcattcgctc tacaaccaca gcctcgccac ctacgacacc ggcgaccagt
     1081 tcgatcacag cgccgccctg ggcttcatca aattatgggg tctgcccctc accacgcaag
     1141 cccaggcgca gctactgcca tcgataggat ttgaaggaaa tctactgaaa ggggcacata
     1201 acgacgaaag gagccttcac ccatgacagc gcgaatctat tacgacaacg atgccgacct
     1261 gtccattatc cagggcaagc gcctcgcctt tatcggctat ggcaaccagg gcgcggcgca
     1321 atcacagaac ctgcgcgaaa gcggcgtgtc ggagattgtg attggcaacc gcgacgataa
     1381 ctataagaag gccgcgctcg acgcgggctt tctggtcgtc tccctcgcgg aggcggctgc
     1441 ctggggcgat gctgtcctcc tgcttatccc cgacgaagag cagccatcgg tcttcagcga
     1501 gcaaatcgcg ccctacttgc ggcccggttc aacgctggtt gtggcctccg gctacaacat
     1561 cgcctttaac ctgctggcca ttccagatgc ggtagatgcc gtgatggttg ccccgcgtat
     1621 gatcggcgca gccgtgcgcg acctctttat gaaaggcagg ccctatccct gcttcgtctc
     1681 gacggagcga gacgtgagcg gcaacgccct cgccctcgcg ctctccatcg cgcgtggcat
     1741 cggggcgaca cgcggcggcg cagtcgcatc ctcggcggtg gaggaggccg cgctcgatct
     1801 cttcagcgag caggcgttct ggccgaccgt cttactggct atgcaatccg cctacgaggt
     1861 cctacacgca gcaggtttca gcgacgaggc catcctgtat gaaatgtatc tatcgaaaga
     1921 gccagccgag gttttcgagc gctttgccga cctgggcatc ttcggccaga tgacgctgca
     1981 ctcgcacacc agccagtacg gacagttgcg cgccctgctc gccgataatg gcgccgcctt
     2041 gcgcgagcgt ttcgcccacg tgctacacga cgacatcctc tccggcgcgt tcgcccgcga
     2101 atggtcagac gtacaggcca aggggccgga gcgcctcgaa caacttcgcg ctgcggcgct
     2161 gaccggctca ctcgcccagg cggaaaagag ggtgatgaaa caataactat tccggcggta
     2221 tacggaaact aaccgccagc cgattccagc cattcataga gacgatagcc agtgtgagat
     2281 agacaagttc ctcatcggtg aagtgcttgc gcgtctcttc atagacatcg tcaggcacat
     2341 ggtcaacggc aatgagcgtc acagcttcgg cccaggccag cgccgcgcgt tcgcgttcgg
     2401 tgaagaaggg ggcctcgcgc catgccacca gcatatagag gcgctgttcg gtttcgccgc
     2461 gcgaccgcgc ggatttggta tgcaggttga ttcaatacgc gcacccattg atctgggatg
     2521 tgcgtatttt gacgagttca atcagcagag gctcctggtc ggccagggca gacgacttga
     2581 ccagttgatc caggccattc atcgctctgc cgatgccatg cacgaccttg ccgtattcaa
     2641 gccggggttg catctccatg ttacatctcc ttgtgcatca ctattgttct cacacaagaa
     2701 gcataacacg atcatacagc cgcaacaagg gaaaatggct ggaggccagg gccatttata
     2761 agtcgagtgg ggcatggaac ctgctcgaat aaaggtaatg ttacgacgtt gactggttaa
     2821 tttccatcaa tacgcacgcc agcacgtccg ccagcagtcc ctgcgccgtc tcgatgccac
     2881 ccgcgcccgg cccaatgatc gtcaccgccg agagcgtatc cgcgtgaatg gtgatagcgt
     2941 tcatcgcgcc atccacgcgc gcgagtgggt ctgagagcgg cagcgccgtc ggctggacgc
     3001 tggcctcaag tgatgcggat tgtccatcca cgccgggatg cagctgcagc gacgcgatca
     3061 gcttgatgcg ctggcccgcg tcggcggcgg cctgcacttg ctgcctgctg atttgagcga
     3121 tgcctacccg ccgcacttcc tctactttga gcgggtgatt gaagaccagc gcggccagaa
     3181 tcatcgtttt ggcaacggcg tcgtagccct ccacgtcggc agttgggtcg ctttcggcgt
     3241 agcccttcgc ctgggcttga gccagcgcat cggcgtagcc ctggccggaa gacatgcccg
     3301 tcagaatata gttggtcgtg ccattgagaa tgccgcgtat ggcgttaact ctggccccgg
     3361 ccatgccctc gcgaattgta ctcaacaccg gcgttccagc catcaccgcc gattccatgc
     3421 gcaactgtac cccgttctgc cgcgccaggg caaacagttc gcttgccgcc agcgcgcccg
     3481 gccccttgtt ggcgctcaca acatgcatcc ggttcgacag cgcgatgcgc atatggctca
     3541 aggccggctc ggcatcgcgc aggttcgtcg gcgatgcctc caccagcgca tccgcttcaa
     3601 ctgcccgcaa tccatccaga gccgtctgcc agtgtgccgc gcctggataa tcggaaagtg
     3661 gtcgcttcgc cgcagcgagc tctagcagcg tcggtagatg caaaccctcc tctc
//
LOCUS       DASZAK010000447         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_650558, whole genome shotgun sequence.
ACCESSION   DASZAK010000447 DASZAK010000000
VERSION     DASZAK010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650558"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VGT44_07790"
     CDS             <1..503
                     /locus_tag="VGT44_07790"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV2580740.1"
                     /translation="YAKWKQPSDRAWRQTQYNYYLVNEVLPFTRQYNPNPYLITAGAS
                     FGAYHAVNFAFRYPQLVDRVIGLSGAYDIKRFTNGYSDDNVYFNNPCDYIQNEHDPAR
                     LDALRHIDIILAIGRDDPSCANNEYFSGILWGKNIWHALRIWDGWVHDWPYWQQMIRM
                     YISGHA"
     gene            523..1557
                     /locus_tag="VGT44_07795"
     CDS             523..1557
                     /locus_tag="VGT44_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580741.1"
                     /translation="MTLKVGLLVGREWSFPPAFIEEVNRRDEGVVAEFVKLGGTRMEE
                     PCPYSVIIDRISHEVPYYRIYLKYAVLQGATVVNNPFMWTSDDKFFEASLATKLGIAS
                     PKTIVLPNKDYVPGIIHDESLRNLLFPLDWYGIVDYVGMPCVLKDALGGGWKDVFICR
                     SMEELLHYYDRFGTRTAILQEFIEWEQYIRCISLGQEEVMPIKYDPHERKYHVEHDHL
                     SPELGRRVVEDSLKLVRALGYDMNSLEWAVRDGVPYAIDFMNPAPDMDIYSLTPFYFD
                     WVVKHMADMAIRLAKHPRPQMRELRWDTLLTAERDRGEDEAATTRVAASPKKANGGRR
                     QTPPPLDITK"
     gene            1618..2970
                     /locus_tag="VGT44_07800"
     CDS             1618..2970
                     /locus_tag="VGT44_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580742.1"
                     /translation="MSLREAIESYHDLLTDELARETQGKLDDQQQLRGITFGGRPLCS
                     VLRPRFMTPEQYHFLESRVRLLLHAFDKAYYLALENEAFRKQFGLLDWEEELVTHNSG
                     FRAPSQTSRVDTFFVHGRGGLRLTEYNAETPASPAYNDALAEVFYGLPVMREYLRRYE
                     VRYMPVRHSVLHALTDAYKQWAGHSEAPRIAILDWREVPSYSEFVLFAEYFKTQGLEC
                     IIADPREVEYRNGRLVAGDFHITLIYKRVLISELVERGGLDQPVVQAVRDGAVCMVNP
                     FRCKILHKKASLAVLSDERNAELFSDAELEAIEAHIPWTCRVEERYAHYHDHAIDLVP
                     FILEHRENLVLKPNDEYGGKGIILGWETDDSRWAQAIQTALSEPYIVQERVTIPSEPF
                     PSIVDGHVTFIDRMLDTNPFVFYGDYMDGCLSRLSTEALLNVSAGSGSAVATFVVEKR
                     "
     gene            2989..>3233
                     /locus_tag="VGT44_07805"
     CDS             2989..>3233
                     /locus_tag="VGT44_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEV2580743.1"
                     /translation="MKPPSLTIGIEEEYQIIDPQTRELRSYITEILEEGRLILREQVK
                     PELHQSIVEVGSSVCQTPADLRTELYRLRRAIMELAG"
BASE COUNT          738 a          892 c          891 g          712 t
ORIGIN      
        1 ggtacgccaa atggaaacag ccctctgacc gggcatggag gcagacgcag tataattact
       61 acctggtgaa cgaagtcctg ccattcacca ggcagtataa ccccaaccct tacttgatca
      121 ccgcaggagc cagctttggc gcgtaccatg ccgtcaattt cgcattccgc tacccccagc
      181 tggttgacag ggtcattggg ctgagtggcg cctatgacat caagagattt accaatggtt
      241 actctgacga caatgtctat ttcaataatc cctgtgacta catccagaac gagcacgatc
      301 cggcgcgcct ggacgctttg cggcacatag acattatcct ggctattggt cgcgacgatc
      361 cttcgtgcgc caacaatgag tacttttcgg gtattctctg gggcaaaaac atctggcatg
      421 cactgcgcat ttgggatggc tgggttcacg actggcctta ttggcaacag atgatccgca
      481 tgtacatcag cggtcacgct tgaccgagag gggagctttc ctatgactct gaaagtcggc
      541 ctgctggtcg gacgagaatg gtcctttccc cccgccttca ttgaagaagt caaccgccgt
      601 gatgaaggtg tcgtcgctga gttcgttaag ctgggcggca cgcgcatgga agagccgtgt
      661 ccctactctg tgattattga ccgtatttcg cacgaggtac catattaccg gatctatctc
      721 aaatacgccg tcctgcaggg tgcgacggtg gtcaataacc ctttcatgtg gacttcggac
      781 gacaaattct tcgaggcgtc cctggctaca aagctgggta tcgccagtcc gaaaactatt
      841 gttctaccaa acaaagacta tgtacccggc attatccatg acgagagcct gcgtaatttg
      901 ctcttcccgc tggactggta tggaatcgtc gactatgttg gcatgccctg cgtgctgaaa
      961 gatgctcttg ggggtggctg gaaagatgtg tttatctgcc gctcgatgga agaattactt
     1021 cattactatg accgctttgg cacgcgaacg gccattttgc aggaatttat cgagtgggag
     1081 cagtatatac gctgtatcag cctcgggcag gaagaagtca tgccgatcaa gtacgatcct
     1141 cacgaacgca agtatcatgt tgaacatgat catctcagcc cagaactcgg gcgccgtgtc
     1201 gtggaggatt cgttgaagct cgtgcgcgca ttgggatacg atatgaattc gctcgagtgg
     1261 gccgttcgcg acggcgttcc ctatgccatt gacttcatga accctgctcc cgatatggat
     1321 atctactcgt tgacgccatt ttatttcgat tgggtggtca agcacatggc cgacatggca
     1381 atccggcttg cgaaacaccc acgcccgcaa atgcgcgaac tgcgctggga taccttattg
     1441 acggcagagc gtgatagggg tgaggatgag gcggcaacaa cccgcgtggc agccagtccc
     1501 aaaaaggcga atggcgggcg caggcagact cctcctcccc tggatatcac caaataaatc
     1561 taaggggcct gctccccacg cgggcgcaat tcattgcgcc tctacaggag gtgtgtcatg
     1621 tcactgcgag aagccataga aagctatcac gatctgctga ccgacgagct agcccgcgaa
     1681 acgcaaggga agcttgacga tcagcagcag cttcggggaa tcaccttcgg cgggcgaccg
     1741 ctgtgcagcg ttctgcgacc acgcttcatg accccagaac agtatcattt cctcgagtcg
     1801 cgggtgcgcc tgctgctgca cgccttcgac aaggcctatt atcttgcatt ggaaaatgaa
     1861 gcctttcgca aacaatttgg cctgctcgat tgggaagagg aactggtgac gcacaactca
     1921 ggcttccgcg cgcccagcca gacctcgcgg gtagacacgt tcttcgtaca tggtcgtgga
     1981 ggattgcgcc tgacggagta taacgcggaa acacctgcaa gccctgctta taatgatgca
     2041 ctcgcggagg tattctatgg cttacctgtg atgcgcgaat acctgcgccg ctacgaggtt
     2101 cgctacatgc ctgtgcgtca cagcgtgctg catgccctga cggacgccta caagcagtgg
     2161 gcaggccaca gcgaagcgcc gcgcatcgcc attctcgact ggcgcgaggt gccgagttac
     2221 agcgagttcg ttctgttcgc ggagtacttc aaaacgcagg gcctggaatg tatcatcgcc
     2281 gacccgcgtg aagtagagta ccgcaacggg cgcctcgttg ccggtgactt tcatattacc
     2341 ttgatctaca agcgcgtgtt gatcagcgag ctggtcgaac gcggcgggct ggatcaacct
     2401 gtcgtgcagg cggtacgcga tggggccgta tgcatggtca atccattccg ctgcaaaata
     2461 ctgcataaga aagccagcct ggccgtgctg agcgatgaac gcaatgccga gctcttcagc
     2521 gacgcggaac tggaggctat cgaagcccac atcccctgga cgtgccgggt cgaagagcgc
     2581 tatgcccact atcacgacca tgcaatcgac cttgtgcctt tcattcttga acatcgtgag
     2641 aacctggtac tcaagccgaa cgacgagtac ggcggcaagg gaatcattct gggctgggaa
     2701 accgacgaca gccgctgggc gcaggcgatt cagacagcat tgagcgaacc atatattgta
     2761 caggagcgag tgaccattcc cagcgagcct tttccgagta tagtcgatgg gcatgttaca
     2821 ttcattgacc gcatgctgga taccaatcca ttcgtcttct acggcgacta catggatggc
     2881 tgcctttcac gtctctcgac cgaggccctg ttgaacgtct cagccggttc cggttccgcc
     2941 gtcgcgacct ttgtagtgga aaagcgctaa gatgagagga gagttgctat gaaaccgcca
     3001 tcactcacca tcggcatcga agaagaatac cagatcatcg atccgcagac acgggaactg
     3061 cgttcttaca tcacagagat actggaagaa ggccgtttga tcctgcgcga acaggtcaag
     3121 cctgagctgc accaatcgat tgtcgaagtc ggttcctcgg tctgccagac gccagccgac
     3181 ctgcgcacag agctgtatcg cctgcgacgc gccatcatgg aactggcagg taa
//
LOCUS       DASZAK010000448         5276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_116902, whole genome shotgun sequence.
ACCESSION   DASZAK010000448 DASZAK010000000
VERSION     DASZAK010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116902"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /gene="argC"
                     /locus_tag="VGT44_07810"
     CDS             complement(<1..1016)
                     /gene="argC"
                     /locus_tag="VGT44_07810"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEV2580744.1"
                     /translation="MSRLRISVVGGSGYTGGELTRLLLFHPQVELAQIVSASHAGQYV
                     QSAHPNLRRLTSLRFAHPDDLTSCDLLLLCLPHGVSAGEIERYRGIAPRVIDLSSDFR
                     LRSPALYERWYGAAHSAPYLLSEAVYGLPELRREELPGASLVSGTGCMATAAILGLAP
                     LYRAGLVNQAIPLVVEAKVGSSAAGASPGISSHHPERSGVVRSFQPTGHRHTAELIQE
                     LGQVSGAGELRQHVAFSATAVELVRGVLVTAHVFVNEAIDERVLWRIYREAYQREPFM
                     RLVKERGGVYRYPEPKILAGSNFCDIGFEYDAEQRRVVVMAALDNLMKGAAGNAVQAL
                     NCMHG"
     gene            complement(1013..1894)
                     /gene="lysX"
                     /locus_tag="VGT44_07815"
     CDS             complement(1013..1894)
                     /gene="lysX"
                     /locus_tag="VGT44_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HEV2580745.1"
                     /translation="MRLAILTSRIRVEEKLLVDALNRRGIDFDIIDDGELLFDLARPD
                     ERWREYDAVLCRSVSQSRGLATLHVLEHWGVRTYNTAAVTALCNDKLLTTLALLRAGI
                     PTPRTLLAYDAQTTVRAIETLGYPAVLKPINGSWGRLLARVNDRDAAEAIIEHQETLG
                     SYQHHIHYAQEYVDKPQRDIRAFVVGDRTICAIYRDSQHWVTNTARGAVTSNCPVTPE
                     LDSLCVNAARAVGGGILAVDVLEDPQRGLLVNEINATMEFRNSIAPTGVDIPAAMLDY
                     ILNDVEAGQLLEEAITR"
     gene            complement(1899..2060)
                     /gene="lysW"
                     /locus_tag="VGT44_07820"
     CDS             complement(1899..2060)
                     /gene="lysW"
                     /locus_tag="VGT44_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HEV2580746.1"
                     /translation="MSNCPECEAEITVQGLMVGEIIFCPDCNAELEVLRLAPPEVALA
                     PQIEEDWGE"
     gene            complement(2470..2970)
                     /locus_tag="VGT44_07825"
     CDS             complement(2470..2970)
                     /locus_tag="VGT44_07825"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV2580747.1"
                     /translation="MKKHDDQAQTGQVKVRVGDSAPDFTLPDQSGAQVSLGDWLGKMD
                     VVLYYYPRDYTAICTDQACAFRDSYQAFLDVGAAVIAISSDSVESHAQFAREHHLPFT
                     LLSDAGGVIRKLYGVPTSFGLPGRVTYIIDRRGIVRHIFFSQFTSKKHVEQALAALKA
                     IREEIV"
     gene            complement(2989..3681)
                     /locus_tag="VGT44_07830"
     CDS             complement(2989..3681)
                     /locus_tag="VGT44_07830"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEV2580748.1"
                     /translation="MSINIDKPPVTPQQYEVLHGGGSEAAEISRERLSRRRFLRRSVL
                     AVFGLSATAAVAGAINMLYPNLAGQFGSVLSAGLKSAFLAATPEKFSVNQAGVFYYQP
                     AKTYIVHMANTTGFSLSGTTLSDQMDAESIVKDADGSYWIALYQRCVHLGCTVPFRND
                     CFSFKCPCHGSHYHVDGEYLDGPAPRSLDRFALSFSGDNVQIDTATLNNKVAHPDATT
                     RLIPVPTVQCTV"
     gene            complement(3688..4611)
                     /locus_tag="VGT44_07835"
     CDS             complement(3688..4611)
                     /locus_tag="VGT44_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2580749.1"
                     /translation="MLSLKEIEDSDTIEVLRELPARSAMNSAINARLAAASEGAYQYY
                     LSDISRYGLLSHDEEIDLARRAAAGSELARRRLVESNLRLVIAIARRYRHTGVPLIDL
                     IQEGNLALMHAVEKFDYRRGCHFGTYATWWIRQAVSRAAGDQSRLIHLPEHVASRLRK
                     LRRVAAQLSQESGMDPLPEQIAEVGQIPLDEVKQLLDLIEQPISLDIPLDEGARTSLA
                     DTIEDGTVPALAETASQNLLSEELYQAMDALTPREQVVISLRFGLGDGWNRTLLEIGR
                     ELGISRERVRQIEEAALNKIRQHYELRVSRK"
     gene            4744..5094
                     /locus_tag="VGT44_07840"
     CDS             4744..5094
                     /locus_tag="VGT44_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580750.1"
                     /translation="MSQQQMNFDADNRDGPPSYETGYQEATHDKDFTSHLPGQKLSWQ
                     DAPMQPTAGQRLALAMVSLLILFLFFLALIVLLTTSTLASNVAQNFAPAFGYMSLGLL
                     AAVIVVNVLFNRKH"
BASE COUNT         1066 a         1561 c         1520 g         1129 t
ORIGIN      
        1 ccatgcatac aattgagcgc ctgaaccgcg ttacctgccg cgcccttcat caagttatcc
       61 aacgcggcca tgacgacgac gcgccgctgc tccgcgtcat actcgaagcc gatatcacag
      121 aagttgctgc ccgccaggat cttcggttcg gggtagcgat acacgccgcc gcgctccttg
      181 accaggcgca tgaatggctc gcgctggtag gcttcgcgat agatgcgcca caggacgcgc
      241 tcatcgatgg cctcgttgac gaagacgtgc gccgtgacaa gcacgccgcg caccagctct
      301 acagcggtgg ctgagaaggc gacatgctgg cgcagttcgc ccgcgccgct gacctgtccc
      361 agttcctgga tcagctcggc ggtatggcga tggccggtcg gctgaaacga gcgcaccacc
      421 ccgctgcgtt cgggatgatg gctgctgatg cccggcgacg ctccggcagc cgacgagccg
      481 actttggcct cgaccacaag cggtatggcc tgattgacca gcccggcgcg gtagagcggg
      541 gcgagtccca gaatggcggc ggtcgccatg cagccagtgc cgctcaccag cgacgcgcca
      601 ggcagttcct cgcggcgcag ttcgggcagg ccgtagaccg cctcgctcag tagatatggc
      661 gcgctgtgag ccgcgccgta ccagcgttca tagagcgcgg gcgagcgcag gcggaaatcg
      721 gaagacaggt cgatcacgcg cggcgcgatg ccccggtagc gctcgatctc cccggcagat
      781 acaccgtgcg gcaggcagag cagcagcagg tcgcatgatg tcaggtcatc gggatgagcg
      841 aagcgcaaag aagtcagcct gcgcaggttt gggtgcgcgc tctgtacata ttgtccggcg
      901 tggctggcgg agacgatctg cgccaattcg acctgcggat gaaagagcag cagccgcgtc
      961 aattctccgc ccgtatagcc ggagccgccg acaacggaaa ttcgcaatcg actcatcgtg
     1021 ttatggcctc ctctaatagc tgtcccgcct ctacatcgtt caggatgtag tcgagcatgg
     1081 cagccgggat atcgacgccc gtcggcgcga tactgttgcg gaactccatg gtggcgttga
     1141 tctcgttgac cagcaggccg cgctgcgggt cttccagcac atcgacggcc aggatgccgc
     1201 cgccgacggc gcgcgccgca ttcacgcaca ggctgtccag ttcgggcgtg acgggacaat
     1261 tgctcgtcac cgcgccgcgc gccgtgttcg tgacccagtg ctggctgtcg cggtagatgg
     1321 cgcagatagt gcgatccccg acgacgaagg cgcggatgtc gcgctggggc ttgtcgacgt
     1381 actcctgcgc gtagtggata tggtgctggt aggagccgag cgtctcctgg tgttcgatga
     1441 tggcctccgc cgcgtcgcga tcgttgacgc gcgccagcag gcggccccac gagccattga
     1501 tcggcttgag gactgccggg tagccgagcg tctctatcgc tcgcacagtg gtctgggcat
     1561 cgtaggccag cagcgtgcgc ggcgtcggga tgcccgcgcg cagcagcgcc agcgtggtga
     1621 gcagcttgtc gttgcacagc gccgttaccg ccgccgtgtt ataggtgcgc acaccccaat
     1681 gctccaacac gtgcagggtt gccaggccgc gcgactggct gacgctgcgg cagagaacgg
     1741 catcgtactc gcgccagcgc tcgtcgggcc gggccaggtc gaagagcagt tcgccatcat
     1801 cgatgatgtc aaaatcgatg ccgcgacgat tgagcgcgtc aaccagcagc ttctcctcta
     1861 cgcgaatacg tgaagtcagg attgccaggc gcatcgactt attctcccca gtcttcttca
     1921 atctgagggg ccagcgccac ttccggcggt gccagtcgca gcacttccag ctcggcattg
     1981 caatcggggc aaaagatgat ttcgcctacc atcagaccct gtacagtaat ttcagcttca
     2041 cattcggggc agttcgacat tacttcgttt ctccttcaat ataaacttgc gttcgccgca
     2101 tcaatcaggg caaataaaaa cgcccttcat cctctcttga gctaaggacg aagagcgcta
     2161 aacattgctc tcgtgatacc accttgtttc gttgatgcct cacgacatca accttgtcga
     2221 gtactaacat actccagccc gcttacgggg gccaccggca gagttctact ctcgcgccag
     2281 gcgatttctt ctcgcagctt caggctttta ttcacagcgt tttatcgtat gggcttttca
     2341 ccaaccgccc actctctggc cgcaatcgca actgttactc gacctatcaa agcttttgtt
     2401 gtattcaatt gtcctgtgtt agacagtaac atgaatgcga ttacaatgtc aagtgttttt
     2461 ggcgcgaatt tagacaattt cttcgcgaat cgcttttagc gcggccagcg cctgctcgac
     2521 atgcttcttc gacgtaaact gcgagaaaaa gatgtgacgc acgatgccgc gcctgtcgat
     2581 gatgtaggtc acgcgccccg gcagaccaaa ggaagttggc acgccgtata gcttgcggat
     2641 cacgccgccc gcatcactca acagcgtgaa cggcagatgg tgttcgcggg caaattgcgc
     2701 atgagattcc accgagtctg agctaattgc gatgacggca gcgccaacat ccagaaaggc
     2761 ctggtaacta tcgcgaaagg cacatgcctg gtcggtgcag atggcggtat aatcccttgg
     2821 gtagtagtag aggacaacat ccattttgcc gagccagtcc cccaggctaa cctgcgcgcc
     2881 tgactggtca gggagcgtaa aatcgggggc agagtcgcca acgcgcacct tgacctgtcc
     2941 tgtttgcgcc tgatcgtcat gctttttcat tgttcttgtt cctatccatt agacggtgca
     3001 ttgcaccgtt ggcacgggga tcaaccgcgt tgtggcatca gggtgcgcca ctttgttgtt
     3061 cagcgttgcg gtatcaatct gcacgttatc tccgctgaag gagagcgcga aacggtccag
     3121 gctgcgcggc gctggcccat ccaggtattc gccgtcaacg tgatagtgcg aaccgtggca
     3181 ggggcatttg aaactgaaac agtcattgcg gaacggcacg gtgcagccca ggtggacgca
     3241 gcgctggtat agggcaatcc agtacgagcc gtccgcgtcc ttgacaatac tttccgcgtc
     3301 catctggtcg ctcagcgtcg tgccgctcaa tgaaaagccg gtcgtatttg ccatatggac
     3361 gatataggtt ttggcaggct gatagtaaaa cacccctgcc tggttaacac taaatttttc
     3421 tggcgtggca gccagaaacg ctgatttcaa gcccgccgac agcaccgaac caaactgacc
     3481 ggccaggttc ggatagagca tattgattgc cccggcaacg gcggcggtcg cggacaatcc
     3541 aaagaccgcc agcacggaac gccgcaggaa gcgtcgtctt gacaggcgtt ccctggaaat
     3601 ttcagccgct tccgaccctc cgccatgcag cacttcgtat tgttgcgggg ttacaggagg
     3661 tttatcgata ttgatactca taacaagtca ctttctagaa acgcgtagtt cataatgctg
     3721 gcgtatttta ttgagcgcgg cttcttcgat ctgccgcacg cgctcgcgcg agatacccag
     3781 ttctctgccg atttcgagca aggtgcggtt ccatccatcg ccgaggccga agcgcaggct
     3841 gatgaccact tgctcgcgcg gtgtgagcgc atccatggcc tggtacagtt cctcgctgag
     3901 caggttctgc gaggcagttt cggcgagcgc gggaaccgtg ccgtcttcaa tggtgtccgc
     3961 gagcgaggtg cgggcgcctt cgtccagtgg tatatcaagc gaaattggct gctcgatcag
     4021 atccaacaat tgcttgacct cgtcgagggg tatctggcca acctcggcga tttgctccgg
     4081 cagagggtcc atgccgcttt cctgcgacaa ctgcgccgcc acacggcgca gcttgcgcaa
     4141 acgtgacgcc acgtgttcgg gcaggtggat caggcgtgac tgatctccgg cggcgcggct
     4201 gacggcctgg cgtatccacc aggtggcata ggtgccgaag tgacaacccc gccgatagtc
     4261 gaatttctcg acggcgtgca tcagcgccag gttgccttcc tggatcaggt cgatgagcgg
     4321 cacgccggta tgccggtagc ggcgcgcgat ggcgatcacc agtcgcaggt tcgactcgac
     4381 caggcggcgg cgcgccagtt cgctcccggc agcggcccgg cgcgccaggt cgatctcttc
     4441 gtcgtgcgag agcagtccat atctgctgat atcgctgagg taatactggt aagcgccctc
     4501 agaagccgcc gcgaggcgcg cgttgatggc gctgttcatg gcagatcgag caggaagctc
     4561 tctcaacact tcgattgtat cgctatcttc aatttctttt agactgagca taataaccgt
     4621 tatcctcttt ttcaccgggt agctgacccg atgtgcatgt atttacaaca atgccactcc
     4681 gctattcgta agtacggtgt acagaaaccc tatgccgtcg aagacaggaa ggaagcattc
     4741 cacatgtcgc aacaacagat gaattttgat gccgataatc gagatggacc accttcctac
     4801 gagacgggat atcaagaggc aactcacgac aaggacttca cctctcactt gcctggacag
     4861 aaactttcct ggcaagatgc gccgatgcaa cctacggcgg gtcaacgctt ggccctggcc
     4921 atggtttctc tgctgatact cttcctcttc tttctcgcac taatagtact gttgactacc
     4981 agcacactag cttccaacgt tgctcagaac ttcgcgcctg cctttggcta tatgagtctc
     5041 ggactcctcg ccgccgtcat tgtggtgaac gtactcttta accggaagca ttaagtttgc
     5101 gcaagctcct ggttaaatcc aatggaagct tggcggactc gtaggagccg gtttaccgcg
     5161 ctcatcgccg attatggttt ttaacttatt aatgcagcaa tgtttacgct caagatagcc
     5221 ggattaataa gttaaaaacc attatcggcc agttcagctg gacgaaaaca ttgcac
//
LOCUS       DASZAK010000449         1623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_650601, whole genome shotgun sequence.
ACCESSION   DASZAK010000449 DASZAK010000000
VERSION     DASZAK010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650601"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VGT44_07845"
     CDS             <1..767
                     /locus_tag="VGT44_07845"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580751.1"
                     /translation="TCIICALDHGMTSPTFLEGLYETRGRTREAIAGGANVFMLGRGI
                     ARQVIEEFTPATSLALMLSASAAGRPQGAVVTPIGSVDEALRLGADAVVVYVALAGEN
                     ESEMIQYASSIGEACDAWGMPFIAEAEFPDAYQTLTESSSTFGAEYLLRNARLCAELG
                     ADIVKVNWSGDQASFEKIVRALKVPVVVAGGTLVSDEELLTRMQQAVEVGAIGCSVGR
                     NIFQHKHPTAITRALCRVIREKWSARDALAELEEQA"
     gene            764..>1623
                     /locus_tag="VGT44_07850"
     CDS             764..>1623
                     /locus_tag="VGT44_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV2580752.1"
                     /translation="MKPATNRYVIGADVGSQGMKTVLLDISGTVVASAYAAYDPRYPA
                     PNWAEEDPAHWEIALTETVRQVMQDAAAQPAEVVALALGSQVDGLVCVGEQDEVLRPA
                     IIWLDRRATAQCDALEEHISAADLFHLTGANLDSSHVAPKMLWVRDHEPAVFERSRYL
                     LLPGSYMAYRLTGETAVDYSNASSTLLLDVKQKAWSQRMLRLTGLDEQRLGHVSGATS
                     VLGPLTKTAAERLGLTTKTVVAVGSGDEHAACVGAGVTGSSTVCDINGTAEPVCAVAR
                     LPVFDEEGLL"
BASE COUNT          308 a          512 c          488 g          315 t
ORIGIN      
        1 gtacctgcat catttgtgcg ctggatcacg gcatgacctc accaaccttt ctagaagggc
       61 tttatgagac ccgcgggcgc acgcgtgaag ccattgcggg cggggctaac gtctttatgc
      121 tcgggcgcgg tatagccagg caggttatcg aggaatttac cccggccacc tcgctggctc
      181 tcatgctctc cgcttccgcc gccgggcggc cccagggagc cgtcgtcact cccatcggct
      241 ctgttgacga agccctgcgc ctcggtgccg atgccgtcgt tgtctacgtg gccctggcgg
      301 gcgaaaacga atctgagatg atccagtacg cctcttccat cggcgaagcc tgtgacgcct
      361 ggggcatgcc tttcatcgcc gaggctgaat tccccgatgc ttaccagaca cttactgaat
      421 ccagcagcac ctttggagcc gagtacctgc tgcgcaacgc gcgcctgtgc gctgagctgg
      481 gtgccgatat cgtcaaggtc aactggagcg gcgaccaggc atcttttgaa aaaatcgtcc
      541 gcgcactcaa ggtccccgtc gtggttgcag gcggcacgct ggtaagcgac gaagagctgc
      601 tgacgcgcat gcagcaggcg gtagaggttg gagctatcgg ctgctctgtt ggccgcaaca
      661 tctttcagca caagcatccc accgccatca cgcgtgcgct gtgccgggtc attcgcgaga
      721 agtggagcgc ccgcgatgcg ctggcagaac tggaggagca agcatgaaac cggcaaccaa
      781 tcgctacgtc attggcgcgg acgttggttc gcagggcatg aagaccgtgc tcctggatat
      841 ctccggtacc gtcgttgcca gcgcctatgc tgcatatgat ccacgctatc ccgcgcccaa
      901 ctgggccgaa gaggaccccg cacactggga aatcgcactc acagaaaccg tgcgacaggt
      961 aatgcaggac gcggctgcgc agcctgctga ggtcgtggcg ctcgccctgg gcagccaggt
     1021 tgatggcctg gtctgcgttg gcgagcagga cgaggtgttg cggccagcca tcatctggct
     1081 tgaccgccgc gctaccgcgc agtgcgatgc actcgaagaa cacatcagcg ccgccgacct
     1141 ctttcacctg accggcgcga acctcgactc ctcgcatgtt gcccccaaga tgctctgggt
     1201 gcgagaccac gagccggccg tttttgagcg ctcgcgctac ctcttattgc cgggcagcta
     1261 catggcctat cgtctcactg gagaaactgc cgtggattat tccaatgcct cttcaactct
     1321 gctgctcgac gttaaacaaa aagcctggtc tcaacgcatg ctcaggctga ctggcctgga
     1381 cgagcagcgc ctgggccacg ttagcggagc aacaagcgtg cttggccccc tcacaaagac
     1441 tgccgctgaa agactgggac tcaccacgaa aacagtcgta gccgttggca gcggcgatga
     1501 gcacgccgcc tgtgtcggcg ctggcgtcac cggcagcagt accgtctgtg acatcaatgg
     1561 cactgccgag ccagtctgtg ccgtagctcg cctgcccgtg tttgacgaag aggggctatt
     1621 gga
//
LOCUS       DASZAK010000450         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_11012, whole genome shotgun sequence.
ACCESSION   DASZAK010000450 DASZAK010000000
VERSION     DASZAK010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11012"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="VGT44_07855"
     CDS             <1..509
                     /locus_tag="VGT44_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV2580753.1"
                     /translation="GAVLGLKDVIFLDYPDGRLQATIELRREIVRVLRKYRPKRLICQ
                     SPERTWEPMMFVGRYHPDHLAAGEAVIQAVYPASQNPWDFPELMEEGLLPHKVNEIYI
                     MGAPHVNYFVDVSETIDKKIEALMCHKSQFIGREQDVEKFVRDRIADLGKKYWVAFAE
                     EFHRTENR"
     regulatory      553..719
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            915..1799
                     /locus_tag="VGT44_07860"
     CDS             915..1799
                     /locus_tag="VGT44_07860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2580754.1"
                     /translation="MSAKTIMPEELEEPGTQQIESGQVITGRTSLAAITRRTRKAQGI
                     AQRSFLIVPPVLLALVILLSWYLGTTIGHIPSFILPAPDAVFAALGDGLSSGLFLSNA
                     LVTIQESLVGFALAVLIGLPLGYGIAKWRLFAITMQPYLAAGQAIPAIVIAPVLYLIF
                     GYGTVPIIILCALIVLFPMVITTALGFQTIDRALIDAARVEGASGWPMLARIEFPLAL
                     PAILAALRTGLTLSVTGALVGEFVSQGSQGLGALVLVSMDQFNVPLNFATLIVLGALA
                     ALYYCASWLLVKLANAIY"
     gene            1835..>2845
                     /locus_tag="VGT44_07865"
     CDS             1835..>2845
                     /locus_tag="VGT44_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2580755.1"
                     /translation="MSSRPRFRLLQPLILLCALLGLLLAGCGTNGSTGSVSTTPTGSL
                     KDVSIALGYIQDIQFAPFYVAQSKGYYQAAGLNVTLHHGIVNDLFGSLGVGHDTFIFA
                     TGDEELVARNSKLPVVDVATIYQRYPVSLIVPASSSIHTLADIKGHTIGIPGPYGSTY
                     VGLQALLYHAHLTTSDVHLQSIGFTQVTALLTHRVDAVVGYSNNEPLQLEKQGMAVRT
                     FDVSDYQPLVSNGIITTESTLSSQPQMVKAFVQATIKGLKEVIANPAEAIQISKSFIP
                     GMNVATETAVLNATIPIWQGNSQTPPGYNDSTTWQSMVQFLVAEKIIPAVPDLTQAYT
                     NLN"
BASE COUNT          578 a          797 c          813 g          657 t
ORIGIN      
        1 ccggagcggt gctgggcttg aaagatgtca ttttcctcga ttatcccgat ggccggttgc
       61 aggccactat cgaactgcgg cgcgagattg tacgcgtcct gcgcaaatat cgtccgaagc
      121 gcctgatctg ccagtcgccg gagcgtacct gggagccaat gatgttcgtt ggtcgctacc
      181 atcccgatca cctggcggcg ggcgaagccg tcatccaggc ggtctatcca gcttcgcaga
      241 atccctggga ttttcccgaa ttgatggagg aggggctgct gccgcacaag gtcaacgaaa
      301 tctacatcat gggcgcgccc cacgtcaact acttcgtcga tgtttccgag accattgaca
      361 aaaagattga agcattgatg tgtcataaga gtcaatttat cgggcgcgag caagacgtgg
      421 agaagtttgt gcgcgatcgc attgctgatc tcggcaagaa atactgggtc gcgttcgcgg
      481 aggagtttca tcgcacagag aatcgctagg ctccccttgc atttccatca tcgtatgtga
      541 catactactt acagagacct tcggggcagg gtgcaattcc cgaccggcgg tatggtctgt
      601 ctctgatgag agaggccaag cccgcgagtc ctggcgctgt tcgctctatc gagcagtcat
      661 gggacagatt cggtgagaaa ccgaagccga cggtatagtc cggatgggag aaggttaagc
      721 gaatctgcgg aagaataagc cgtgtcgcct tttcgtgtcg acagggtgag ttcgtctatc
      781 tatccgtcga tgggtagaat ccgttttgct ctccttttgt ctccctcaca tgcctcccag
      841 ggttagtaac attgacagtc cctgttgtct tttcggttgt ctgactgtca gtttaactac
      901 gaccgaggga agttatgagc gcaaagacaa tcatgccaga ggaattggaa gagccaggca
      961 cacagcagat tgaatcagga caggtgatta ccgggagaac atctctcgcg gctattacca
     1021 ggcgcacaag gaaggcgcag ggcatcgcac agcgatcctt cttgatcgtg ccgcccgtgc
     1081 tactggcgct tgttatcctg ctgagctggt acctgggtac cacaattggg catatcccca
     1141 gcttcatttt gcccgcgccg gacgctgtct tcgcggccct gggggatggc ctgagttcgg
     1201 gtttgttcct cagcaacgcg ctagtcacaa tccaggaaag cctggtgggc ttcgctctgg
     1261 cggtgctgat tgggctgccg ttgggctatg gcatcgcgaa atggcggctg ttcgccatca
     1321 ccatgcagcc ctacctggcg gcaggccagg caatcccggc catcgtcatc gctcccgtac
     1381 tgtacctgat ctttggctat ggcaccgtgc cgatcatcat cctctgcgcg ctgatcgtgc
     1441 tgttcccgat ggtgattacg accgcgctcg gtttccagac catcgaccgg gcgctgatcg
     1501 atgcggcgcg cgtcgagggc gcatcgggct ggcccatgct ggcgcggatc gagttcccgt
     1561 tagcgctgcc cgctatcctg gctgccctgc gcaccggcct gacgctgtcg gtcactggcg
     1621 cgctggtggg cgagtttgtt agccagggat cgcagggatt gggcgcgctg gtgctggtgt
     1681 caatggacca gttcaatgtg ccgctaaact tcgccacgct gattgttctg ggcgctctgg
     1741 ctgcgttgta ctactgcgct tcctggctgc tggtgaaact ggccaatgct atctattaga
     1801 caacaggcta accgacgaat ggagagaaat atcgatgtct tcccgtcctc gttttaggct
     1861 gcttcagcca cttattctgc tgtgcgcttt gctgggactg ctcctggcag gctgtgggac
     1921 aaatggatca accggaagcg tttcaacgac gccgactggc tcgctgaaag atgtttcaat
     1981 cgccctgggc tatattcaag atatccagtt cgcgccgttc tatgtcgcgc agagcaaggg
     2041 atattaccag gcggcagggc taaatgtaac gcttcatcat ggcatcgtca atgacctgtt
     2101 tgggtcgctt ggggttggtc acgatacgtt catttttgcc accggcgacg aggaactggt
     2161 ggcgcgcaac agcaagttgc cggtggtaga tgtcgccacg atataccagc gctaccccgt
     2221 cagcctgatc gtcccggcca gctcatccat ccacacgctg gcggacatca aggggcatac
     2281 catcggcatc cccggaccct atggctcaac ctacgtcggc ttgcaggcgt tgctgtacca
     2341 tgcgcatctt acaacctcag atgttcacct gcaatccatc ggttttaccc aggtgacggc
     2401 gttgctgacg catcgcgttg acgccgtggt gggctactcg aacaacgagc cgctccaact
     2461 ggagaagcag ggcatggcgg tgcgcacctt cgatgtgtct gattatcagc cgctggtctc
     2521 caatggtatt attaccaccg agagtacgct gagcagtcag ccgcaaatgg tcaaagcttt
     2581 cgtacaggcc acgatcaaag gtctgaaaga agttatcgca aatccggcgg aagccatcca
     2641 gatcagcaaa tccttcatcc ccggcatgaa cgtcgcgacc gagacagccg tcttgaatgc
     2701 gaccatccct atctggcagg gcaacagtca gacgccgccc ggctacaacg acagtactac
     2761 ctggcagtcg atggtgcaat tcctcgtcgc ggagaaaatt attcctgccg tgccggatct
     2821 cacacaagca tatacgaatc ttaat
//
LOCUS       DASZAK010000451         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_187401, whole genome shotgun sequence.
ACCESSION   DASZAK010000451 DASZAK010000000
VERSION     DASZAK010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187401"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGT44_07870"
     CDS             complement(<1..369)
                     /locus_tag="VGT44_07870"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV2580756.1"
                     /translation="MIPSASHNNTTLVLRRTFTASRQRVFRAWITPRALEHWLRPRGM
                     SMTVRSLDVRVGGSFCFEIEDGSCIVGTYLHIVPPEKLVFTWSGGAAQSWGTVVTLDF
                     LDQGPVTEIVLTHEGLSTPER"
     gene            complement(347..715)
                     /locus_tag="VGT44_07875"
     CDS             complement(347..715)
                     /locus_tag="VGT44_07875"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2580757.1"
                     /translation="MVNDSSERLTTVFLALADPTRRAILERLAHGEASGTELARPFSI
                     SVPAISKHLRVLEHADLILHRKDGRTHRFRLAARPMRDAAAWLEHYRQFWDTQFDALD
                     TYLQVTSEEEQDPDDPFSIS"
     gene            complement(766..>1808)
                     /locus_tag="VGT44_07880"
     CDS             complement(766..>1808)
                     /locus_tag="VGT44_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2580758.1"
                     /translation="RHAVVVLAATNRVDILDKALLRPGRFDRQVTVSLPDRIGREAIL
                     KVHTRNTPLHEEVNLARLSRMTTGMSGADLANLVNEAALCAARRDQDCLTHNCFEDAL
                     ARVQLGALRPLVMNETERRIIAFHEGGHALVAHHLPLADKVNRVTILPRGQSLGVTQF
                     IAEEDRYNYSRETLMARIAVGLGGRVAEELTFGPERVTTGAENDLQVVTDLARRMVTR
                     WGMSEQVGVVFADHRSELAGAGLNMIGVDVPAQSRSLVADANGRLLLNGGSVPASHHA
                     FAMVSPTASAGSSASMAALIDAEVKRILNEGREIARTILSEHYDQLTLLAEKLMEHEQ
                     LDRAQFEELLRE"
BASE COUNT          366 a          516 c          550 g          376 t
ORIGIN      
        1 tcgctccgga gtgctcagcc cttcgtgggt cagcacaatt tccgtcaccg gaccctggtc
       61 gagaaagtcc agcgtgacga cggttcccca gctctgcgct gctccgccag accaggtaaa
      121 gaccagtttt tcgggtggaa cgatgtgcag gtaggtgccg acgatgcagc tgccgtcctc
      181 tatctcaaag cagaaggacc cgccaacccg cacgtcgagc gaacgcacgg tcatgctcat
      241 gcccctgggt ctcaaccagt gctccaacgc ccgaggcgtg atccaggcgc gaaatacccg
      301 ctggcgtgag gcggtgaacg tccgtcgcag cacgagggtg gtgttgttat gagatgctga
      361 agggatcatc agggtcttgc tcctcttctg atgtaacttg caggtaggtg tccaatgcgt
      421 cgaattgggt atcccaaaac tgccggtagt gttcaagcca ggcggcagcg tctctcatgg
      481 gacgggcagc caggcgaaac cggtgcgtgc gtccgtcctt gcggtgcaga ataagatcag
      541 cgtgttccag cacgcgtaaa tgtttggaga tcgctggcac ggagatcgag aatggccgcg
      601 ccagttctgt tccagaagcc tcaccgtgcg ccaggcgttc taaaatggcc cggcgtgtag
      661 ggtcagcgag cgccaaaaag acggtagtaa gtcgctctga tgaatcgtta accatacagt
      721 taattatacg aagattgtga atcgttgtca atggcccgca acgcttcact cgcgcagcag
      781 ttcctcgaac tgtgcccggt cgagttgctc gtgttccatg agcttctcag cgagcaaggt
      841 gagttgatcg tagtgctcgc tgagaatggt gcgggcaatt tctcgtcctt cgttgagaat
      901 acgttttacc tcggcatcta tcagggctgc catgctcgcg ctgcttccag cggacgcggt
      961 gggagagacc atggcaaacg catgatgaga agcgggcaca ctgccaccat tcagcaggag
     1021 acgaccgtta gcgtcggcta ctagtgagcg agactgcgcg gggacatcca caccaatcat
     1081 gttaaggcca gctccagcaa gctcggagcg atggtcggcg aagacgactc caacctgctc
     1141 gctcatgccc cagcgtgtga ccatgcgccg cgccaggtcg gttaccactt gcagatcgtt
     1201 ctctgctcct gtcgtcactc gttcggggcc gaaggtcagt tcctcggcga cacgtccgcc
     1261 caggccgaca gcaatacgcg ccattaacgt ctcacggctg tagttgtagc ggtcttcctc
     1321 tgcgatgaac tgcgttaccc ccaggctctg gccacgcggc aggatggtga cgcggttcac
     1381 tttgtccgcc aggggcaggt ggtgagcgac gagggcatgc ccgccttcgt ggaaggcgat
     1441 gatgcgacgt tccgtctcgt tcatcaccag gggtcgcaat gcacctagct ggacacgcgc
     1501 cagtgcatcc tcgaagcagt tgtgggtgag acaatcctgg tctcgacgtg cagcacacag
     1561 cgcagcttca ttaacgaggt ttgccaggtc agctccgctc atgccggttg tcatacgtga
     1621 gaggcgagca aggttgacct cctcatgtaa gggagtgttg cgggtatgga ctttgaggat
     1681 cgcctcacgc ccaatccgat ctggcagtga gacggtgacc tggcgatcaa agcgcccggg
     1741 gcgcagtaac gctttatcaa ggatatcgac cctattagtt gcggccagca cgacgacagc
     1801 gtggcgtg
//
LOCUS       DASZAK010000452         1601 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_437719, whole genome shotgun sequence.
ACCESSION   DASZAK010000452 DASZAK010000000
VERSION     DASZAK010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437719"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..755)
                     /locus_tag="VGT44_07885"
     CDS             complement(<1..755)
                     /locus_tag="VGT44_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580759.1"
                     /translation="MAKTTPIVRGNTLMYQQNGQEQALLVETPAWYAWLESASSFAFT
                     SQAGTFTARKERAGNLRGGWYWKAYRTRRGRLTSHYLGKPETITLARLQTVAQALADA
                     LVETASKNDTDAVVLPAQAATPGMQSDSLTPLLATKLHPPLPRASLVRRPHLAARLTQ
                     GVMGPLTLVSAPAGFGKTTLLAQWLAESGMPVAWLSLEPGDNEPVRFLSYLIAALQAL
                     DPRLGAVALTLLHMPQPAAAETVLTLLTNDVGS"
     gene            1027..>1601
                     /locus_tag="VGT44_07890"
     CDS             1027..>1601
                     /locus_tag="VGT44_07890"
                     /inference="COORDINATES: protein motif:HMM:NF018239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XylR N-terminal domain-containing protein"
                     /protein_id="HEV2580760.1"
                     /translation="MKAIDLLGSQFEHLANEGKISAHGRRVLLIDTASFDALRAELIG
                     FLGQDEARGSLYRLGYSMGHNAARRLRDRYHWNDEREWMSACPVLMNWSGLADTKLNR
                     LEFDRSRGAFLGELSWTGSFESEQHLRQSGIAQTPICWMLTGYVSGYFSYAAGQSLLC
                     LETDCVGKGDTQCVALVKPIAVWGDAARKSA"
BASE COUNT          294 a          452 c          518 g          337 t
ORIGIN      
        1 tgacttccca cgtcgttggt gagcagcgtg agtacggtct ctgctgcggc aggttgtggc
       61 atgtgcagca gggtcaatgc caccgccccg agacgtgggt caagcgcctg gagggcggca
      121 atgagatacg agaggaagcg caccggctcg ttgtcccctg gttcgagtga gagccaggcg
      181 acaggcatgc cgctttcggc gagccactgc gcgagcaagg tcgttttccc aaagcccgcg
      241 ggtgccgaga cgagcgtgag cggacccatc actccctgcg tgagtcgtgc agccagatgg
      301 gggcggcgca cgagggacgc gcgtggcagg ggtggatgga gcttggtggc gagcagcggg
      361 gtgagtgaat cgctctgcat cccaggtgtt gcggcttgcg caggaaggac aacagcgtcc
      421 gtatcgtttt tagacgcggt ctcgacgaga gcatcagcca atgcctgggc caccgtctgt
      481 aaacgcgcca gcgtgatggt ctcgggcttg ccgaggtagt gggaggtgag cctgccacgc
      541 cgtgtgcggt aggctttcca gtaccagccc ccgcgcaggt tgcccgcgcg ctctttgcga
      601 gccgtgaagg tgcctgcctg gctggtaaag gcaaagctgg aggcggactc caaccaggca
      661 taccaggcgg gcgtttccac caggagcgcc tgctcctgcc cattttgctg atacatcagc
      721 gtgtttcctc gtacaatggg tgtcgtcttc gccatggttc ctctcccgtg atatggaacg
      781 tcgaacgcgt tcctttcccg aactggaatg ccgatgttcg gtaattcata cggcataccg
      841 aacgaggagt ccagcgatct ttaagatagg tctatcttat catctttcct ttggcctgaa
      901 cattctctgg gcaaaaaagt gtggaccccc ccttgatggg acaagcttga caagcctgcc
      961 ttcctgggat acgctcaatt caacgggatg tatatatttg ttgcgcagga atgggcgttt
     1021 cgcaacgtga aagccatcga tttattaggt agccagtttg aacacttggc gaacgaaggg
     1081 aaaatcagcg cgcatggccg ccgtgtgctg ctgatcgaca cggcctcgtt cgatgccctg
     1141 cgcgccgagt tgatcggctt tctgggccag gacgaggcgc gcggctcgtt gtaccggctc
     1201 ggctactcga tgggtcataa cgccgctcgc cgcctgcgcg atcgctacca ctggaacgat
     1261 gagcgggagt ggatgtcagc ctgcccggta ctcatgaact ggtcggggtt ggccgatacg
     1321 aaattgaatc gtctcgaatt tgaccgctcg cgcggcgcgt ttctggggga gctttcctgg
     1381 acgggatcgt ttgagagcga gcagcacctg cgtcaatcgg gcatagctca gacgcctatt
     1441 tgctggatgc tcaccggcta cgtcagcggc tacttcagct acgcggcggg ccagagcctg
     1501 ctctgcctgg agaccgactg cgttggcaag ggcgatacgc aatgcgtcgc cctcgtcaaa
     1561 cctatcgccg tctggggtga cgctgcccgc aaaagcgcct t
//
LOCUS       DASZAK010000453         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_153149, whole genome shotgun sequence.
ACCESSION   DASZAK010000453 DASZAK010000000
VERSION     DASZAK010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153149"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VGT44_07895"
     CDS             complement(<1..1616)
                     /locus_tag="VGT44_07895"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2580761.1"
                     /translation="MHPHESIDWYPIVKRYTTGLVHIRSVSPGKGEIHVAREVLRLLQ
                     EDGLESSYTAIGLDPLIDDPAGRCNAYAFLRGKHPQTIVLLGHIDTVATGDFGALEPF
                     ALDPGALAAHVDQLAAVAPGLAQDLAAHPGDWMLGRGSGDMKSGDAVNIAVMRRLAVL
                     AREDRLELSVVLLATPDEENESAGVLQAVQFLLRLREQYGLEYLGAINTDYVTALYPG
                     DPHRYIYTGTVGKLLPTFLVIGQPSHVGDPFDGLDANLLASELIRDLSMNDELCDHVR
                     GQSTPPPVTLKFTDLKTSYDVQLPFASYFYLNVLTLSTTPAQLLDKLVHRAQSVMAQV
                     LRRVDETERRWKSRRPPVYQDTPPRQRTGEVFTYARLYQDLKQRLSEQRMDAEIAAAW
                     ESLPAGLDARGRCLHLVLHLWSLSGSRGPAIVVYYSPPFIPHIAATPGPLHEAVHAVA
                     AAHTELHLEVQEYFPLISDMSYLRLDPEIDTGALIANLPIWDDAPAPGAPARPGTYTL
                     PLQAIRQLNLPVVNLGPYGKGAHQAGERVL"
     gene            1915..>2101
                     /locus_tag="VGT44_07900"
     CDS             1915..>2101
                     /locus_tag="VGT44_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580762.1"
                     /translation="MPDTTASVSRSIGLMAKPPGTRLKWMRAAACTLAGGKPALARAF
                     DSAFEYQPTCVAQNGEQK"
BASE COUNT          395 a          546 c          731 g          429 t
ORIGIN      
        1 atcagcacac gctcgcctgc ctggtgcgct cctttaccgt aagggcccag gttgacgacg
       61 ggcaggttga gctgccggat ggcctgcagg gggagcgtat atgtgccggg acgcgcggga
      121 gcaccaggcg ccggggcatc gtcccagatt ggcaggttgg cgatgagcgc gcctgtgtcg
      181 atttcgggat cgaggcgcag gtagctcata tcggagatga gtgggaagta ctcctgcacc
      241 tcgaggtgca gttctgtgtg ggctgctgcg actgcatgca cggcttcgtg cagcgggccg
      301 ggtgttgcgg cgatgtgcgg gatgaagggc ggggagtagt agacgacgat ggcagggccg
      361 cgactgccgc tcaatgacca gagatgtagc acaaggtgca gacagcgccc gcgcgcatcg
      421 agtccggctg gcagggattc ccacgcggct gctatttccg catccatgcg ctgctcgctc
      481 aggcgctgct tgaggtcctg gtagagccgg gcgtaggtga acacctcgcc agtacgctgt
      541 ctgggtggtg tgtcctggta gacaggaggg cgtctgctct tccacctgcg ttctgtttcg
      601 tccacgcggc gcagtacctg tgccattacg gattgagcac gatggacgag tttatcgagc
      661 aactgcgcgg gtgtcgtcga gagggtgagc acgttcaggt agaaatagga ggcgaagggc
      721 agttgcacgt cgtagctcgt tttgaggtcg gtgaacttta aggtgactgg cggtggcgtc
      781 gattggccgc gcacatgatc gcagagttca tcgttcatgc tgaggtcgcg gatgagttcc
      841 gatgcgagca ggttggcgtc gaggccgtcg aaggggtcgc cgacgtgcga gggctggccg
      901 atgacgagga aagtgggcag gagcttgccg acggtgccgg tgtagatata gcggtgcggg
      961 tcgccgggat agagcgcggt aacgtagtcg gtgttgatag cgccgagata ctccaggcca
     1021 tattgttcgc gcaagcgcag gagaaactgg acagcttgca gcaccccagc ggattcgttt
     1081 tcttcgtcgg gcgtggcaag caagacgacc gagagttcta gcctgtcctc tcgcgccaga
     1141 actgccaggc ggcgcattac agcgatattc acggcgtcgc cgctcttcat gtcgccagag
     1201 ccgcgtccta acatccagtc gcccgggtgt gcggccaggt cttgcgctaa accgggcgct
     1261 actgccgcta attgatcgac atgggcagcc agcgcgccgg ggtcgagcgc gaatggttcg
     1321 agcgcgccga aatcgccggt agcgacggta tcgatatgac cgagcaagac aatggtctgg
     1381 ggatgcttgc cgcgcaggaa ggcataggca ttgcatcgcc cggcaggatc gtcgatgagc
     1441 gggtcgaggc cgatggcggt gtaggaagat tcgagtccgt cttcttgcag caagcgcagg
     1501 acttcccggg ccacgtgaat ttcgcccttg ccagggctga cgctgcgaat atgcacaagc
     1561 ccggttgtgt agcgtttgac gatggggtac cagtcgatcg attcgtgtgg gtgcatgagt
     1621 atagtgtgtt cgcctttccc tggatggaat agtctgattc ctgctgatgt attgtaccag
     1681 atatgaaaat gcgctgggct tgcacaagga gatgtacatc ccagcattaa ttgatcccga
     1741 agcccgactt ccccaaatcg ggctggcacg gctctcgtct atggctggaa gctgcgcgtc
     1801 ctctctgttg ccattttagg gggttgcatc atggctatcg aagagcagca gattgaggac
     1861 acttggagta tctgaacttc gcccgaaaaa gtgtgggggg gagatggttc ccctatgcct
     1921 gacaccacgg cttcggtctc caggtcgata gggttgatgg cgaaaccgcc gggcacccgg
     1981 ttgaagtgga tgcgcgcagc tgcgtgtacg ctggctggcg ggaagccagc acttgcgaga
     2041 gcgttcgaca gcgccttcga gtaccagccg acgtgcgtcg cgcaaaacgg agaacagaag
     2101 c
//
LOCUS       DASZAK010000454         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_11088, whole genome shotgun sequence.
ACCESSION   DASZAK010000454 DASZAK010000000
VERSION     DASZAK010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11088"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VGT44_07905"
     CDS             <1..379
                     /locus_tag="VGT44_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV2580763.1"
                     /translation="PGFQSLEVLGDEHWKAVVAVGIGAVKAKFTLDVTRPEMREPEEM
                     IVKGRGKAPGSAVDLSGDMRLTALDSEQTQMDWKATVVVSGTIASVGARLMQGTAERL
                     TGQFFDCLKTTLQSPDPAAKANS"
     gene            399..1265
                     /locus_tag="VGT44_07910"
     CDS             399..1265
                     /locus_tag="VGT44_07910"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2580764.1"
                     /translation="MESAIERWQAIIDARARQMDAAYARLGRTSADFWNRRARGYHRS
                     TKDTVSNDPFYRYLIKVISPATTVLDVGAGTGRFALALAPLVRQVIAVEPNAAMLDYL
                     RQDAQERGITNISYSQSTWQDTPNEVQADIVICSHVLYPIRDIAPFLAKLHHAAGQAY
                     YIYMRATPMDTLTAPLWKHFHGDDRCMPPGYIHALDVLYEMGLYANVEVVNLPPTMRF
                     PSLDRAVEELLEQLILPDDQPTREELRSLLQGWLVEQDDALVPPAQEMVCGILYSTTF
                     RTIYNGLARTSS"
     gene            complement(1211..2182)
                     /locus_tag="VGT44_07915"
     CDS             complement(1211..2182)
                     /locus_tag="VGT44_07915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2580765.1"
                     /translation="MTTTHQQAQQVETAPETTLTYSIIAPVFNEEATLPHYYQRVVAV
                     MEEVGGTFELIFVNDGSRDASYQKMLALHASDPRVRVLDFSRNFGHQIAISAGLDYAR
                     GQAVIIMDTDLQDPPEVIPRMIECWKGGAEVVYAQRATRKGETRFKLMTAAAFYRLIA
                     RLTSVSIPRETGDFRLIDRSVVNALITMREHHRFMRGLSSWVGFRQEAIVYDRQERFA
                     GSTHYPLGKMISFSLDAITSFSHIPLQLATTCGFFFAGISLVGIVIAVLLRLITGAIV
                     GQASTLIMVLFLGGIQLIFLGIIGEYLGRIYDEVRARPLYIVRKVVE"
     gene            complement(2434..2868)
                     /locus_tag="VGT44_07920"
     CDS             complement(2434..2868)
                     /locus_tag="VGT44_07920"
                     /inference="COORDINATES: protein motif:HMM:NF017999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV2580766.1"
                     /translation="MKLNGTHKFQANSKQVFNAIVNPDVLKSCIPGCNSVEILDPTRL
                     KASITTPLPGLKGPFGVVIKMTQRQDPNLLVLEVNRKGTGGSVKATSQISIQDQPDGA
                     LLTYDANADLEGPIAIANNPIGQTITKNSLKNFFDKLEKAIV"
BASE COUNT          669 a          813 c          905 g          689 t
ORIGIN      
        1 gccgggcttt cagagcctgg aagtgctggg cgatgaacac tggaaagcgg ttgtcgcagt
       61 tggcatcggc gcggttaagg ctaagtttac cctcgatgtg acgcgccctg agatgcgcga
      121 gccggaggag atgatagtga agggtcgtgg caaagcgcca ggtagcgccg tcgatctatc
      181 cggtgatatg cgcctgaccg cgctcgacag cgaacagaca cagatggatt ggaaggcgac
      241 ggtggttgtg agcggcacga ttgccagcgt cggcgcgcgt ttgatgcagg gtacggcgga
      301 acggctgacg ggtcaattct tcgattgcct caagacgaca ctccagtcgc ccgatccggc
      361 ggctaaggcg aacagctagc gctggagcat cgaatctcat ggaatccgcg attgagcgct
      421 ggcaggcaat catcgatgcg cgcgcgcgcc agatggacgc ggcctacgcg cggctgggtc
      481 gcaccagcgc tgatttctgg aatcggcgcg cgcgcggcta tcaccgttca acaaaagata
      541 cggtctcaaa cgacccgttt tatcgatacc tgataaaggt tatctctccc gccacaactg
      601 tgctggatgt tggcgcggga acgggccgtt ttgccctggc gctcgctccg ctggtcaggc
      661 aggtgatcgc cgttgagccg aatgccgcca tgctggacta cctccgccag gatgcgcagg
      721 aacggggcat caccaatatt tcctatagtc aatcgacatg gcaggatacg cctaatgagg
      781 tgcaggctga tatcgtgatt tgcagccatg tcctctatcc tattcgcgat atcgcgccgt
      841 ttctcgccaa actccatcat gccgcagggc aagcgtacta catctatatg cgcgcaacgc
      901 cgatggatac attgaccgcg ccactctgga aacattttca cggcgacgat cgctgtatgc
      961 cacccggcta cattcacgcc ctcgatgttc tctacgaaat gggtctctat gccaacgtcg
     1021 aggtggtgaa tctgccgcca accatgcgat tcccctcgct cgacagggcg gtagaggagc
     1081 tgctggaaca gctcatcctg cccgacgacc agccgacgcg cgaagaactg cgaagcctgt
     1141 tacagggctg gctggttgag caggatgacg cgcttgtgcc tccagcgcag gagatggtgt
     1201 gtggaatcct ctattcgacc acttttcgca cgatatacaa cggtctcgcg cgcacctcgt
     1261 cgtagatgcg gcccaggtat tcgccgatga tgcccaggaa gatcagctgt atgccgccca
     1321 ggaacagcac catgatgagt gtggacgcct ggccgacgat ggcgccggtg atgaggcgca
     1381 gtagcacggc gatcacgatg ccgaccaggc tgatgcctgc gaagaagaat ccacaggtgg
     1441 tggccagttg gagggggatg tggctgaagc tggtgatggc gtccagcgaa aacgagatca
     1501 ttttgcccaa cgggtaatgg gtcgaacccg cgaatcgctc ctgccgatca tagactatcg
     1561 cttcctggcg aaagccaacc cacgacgaca ggccgcgcat gaaacggtgg tgttcgcgca
     1621 tggtgatcag cgcattgacg acgctgcggt cgatcaggcg aaaatcgccg gtctcgcgcg
     1681 ggatggacac cgaagtcaga cgcgcgatca ggcgatagaa cgcggcagcc gtcatcagtt
     1741 tgaagcgcgt ttcccctttg cgtgtggcgc gttgcgcgta gaccacctcc gcgccgcctt
     1801 tccagcattc gatcatgcgc gggatgacct ccggcgggtc ttgcaggtcg gtatccatga
     1861 tgatgacagc ctgaccacgc gcgtagtcga ggccggccga gatggcgatt tgatgcccaa
     1921 aattgcgcga gaagtcgagg acgcgcacgc gcgggtcgct ggcgtgtaat gcaagcattt
     1981 tctgatacga agcatcccgg ctgccgtcat tgacgaagat caactcgaag gttccaccta
     2041 cctcttccat gacggccacg acgcgctgat aatagtgcgg aagcgtcgcc tcctcgttga
     2101 agaccggggc aataatcgaa taggtaagcg tcgtttcagg cgccgtctca acctgttgcg
     2161 cctgttgatg tgttgttgtc atcgtagaat tctccagctt tctctccgcg atgagtgttt
     2221 ccagtgtttg catagggcct gctccaattc ccttgtatag taaatcataa cgtgctatag
     2281 aaagaaatgt ttgtaagcaa tctgcttact ttgaggatag atgatgtgga ttgaagggag
     2341 attggaagag gatgtgaata ttctcacgat aggcacaagc aaagaacctg gcaaccataa
     2401 tggttgccag gttctttgct tgtgccttga atcctagacg atagcctttt ccagcttatc
     2461 gaagaagttc ttgagcgagt ttttggtgat agtctggccg atggggttat tggcgatggc
     2521 gattggcccc tccaggtcgg cgttggcgtc gtatgtgagc agcgctccat cgggctgatc
     2581 ctgaatgctg atctggctgg tggccttgac ggaaccgcct gtacctttgc ggttcacctc
     2641 caacacgagc aggttgggat cctggcgctg ggtcatctta atgacgacgc caaagggtcc
     2701 tttgaggccg ggcagcggcg tggtaatact cgccttcagg cgggtgggat cgagaatttc
     2761 aactgagttg caaccgggaa tacaggattt cagcacatca ggattgacaa tagcattaaa
     2821 aacttgcttg ctattcgcct ggaacttgtg ggtaccgttg agtttcatat agtccttcct
     2881 aaatagtcaa aaaaaacgtt ttggcaggtt cttgatctcc cctcaagatt gccagtatcg
     2941 tcagaagtat ttcagagggc attgtaacct gtatgaatca tttgtacaag ctttgagcat
     3001 cgtcttacca atttttccta gagccaccgg tacaaatgcc tatggttaag gccgatcaac
     3061 tatgctaccc aaatgt
//
LOCUS       DASZAK010000455         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_402100, whole genome shotgun sequence.
ACCESSION   DASZAK010000455 DASZAK010000000
VERSION     DASZAK010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402100"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..781)
                     /locus_tag="VGT44_07925"
     CDS             complement(128..781)
                     /locus_tag="VGT44_07925"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEV2580767.1"
                     /translation="MTQDFEANRKQLIESLRASGIEDARTLAAVASVPREYFVDEAYA
                     DLAYEDRALPITMGQSISQPLMVATMTQALQLRGQERVLEIGTGTGYQTVILSRMAAW
                     VYSVERIQQLAAVASMRLSKLGVSNVTIYVGDGSLGWPDQAPYDRILVTAAAPDIPVR
                     LYEQLVTWGIMVIPVGGSDHQELLAVHRAPWGQEKRRLGGCMFVPLVGEEGWHLRSQ"
     gene            1048..2439
                     /locus_tag="VGT44_07930"
     CDS             1048..2439
                     /locus_tag="VGT44_07930"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEV2580768.1"
                     /translation="MHHSLPDLLAQPHAAVPFFETVLVQALPADPPTPKGSPRGAPQK
                     LSWMHLWFGFVLGILQGVASYQGVQRLLAADPLGPFAKVVLTDDALVKRLRQAGIEPL
                     QQMLARLSAVLAPLLRPAFPADLAAFAREILALDETTWEAVQRHLAVLRVVPKGDERL
                     VPGKLAGRFNVRTQQWDLIQLRTNPVGNCKLDLCSLLEGLLADSLLLFDLGYFSYPFF
                     DYLTQRHFWFISRLREKVCYQIVHEFYRQAGTLDALVWLGSTGRNSPRTGCLLRLVRF
                     WDGHELRMYLTNQTDPLALPLPDIARLYARRWDIELAFLTLKAHLGLHHWWSGHPVLQ
                     QQQALVVLIAAQLLHALRVLIALDAGCDPFEVSLPLLVEYLPQILHQRLHPVSWAMQH
                     GDDLGLFRPSSRYVVQAPVVPTTALCFPPADLPRTRLACYLTYKQRPHRSPRKKPRRS
                     PASSSRASPRLDK"
BASE COUNT          432 a          815 c          685 g          530 t
ORIGIN      
        1 gtcgcccact gtccgatacc agggcgaccg caagagtcgc ccactgcccg ataccagggc
       61 gaccgcaaga gtcgccctta ccctctatta tagtatgttc gacgaatagt acaagaggga
      121 agttacctta ttggctgcgc aggtgccagc cttcttcgcc gaccagcggt acaaacatac
      181 acccgcccag gcggcgcttt tcctgccccc agggcgcgcg atgtacggcc agcaattcct
      241 ggtgatctga tccgcctacc gggatgacca tgatgcccca ggtgacaagc tgctcataca
      301 ggcgcacggg gatgtcgggc gcggcagccg tgaccaggat gcggtcatag ggggcctggt
      361 ccggccagcc cagcgagcca tcacctacat agattgtaac gttgctgacc cctaattttg
      421 acagacgcat cgaggccacc gccgcgagct gttggatgcg ttcgacgctg tagacccacg
      481 cggccatccg ggagagaata accgtctggt agccggtacc cgtcccaatt tccaagacgc
      541 gctcctgtcc ccgcagttgc agcgcctgcg tcatggtcgc taccatcaag ggctgtgaga
      601 tactctgccc catagtgatt ggcagcgcgc gatcttcgta agccaggtct gcgtacgctt
      661 cgtccacgaa gtattcgcgc ggcacactgg cgacggcggc gagcgtacga gcatcttcga
      721 tacccgacgc gcgcagtgat tctatgagct gcttgcggtt cgcctcgaaa tcctgggtca
      781 tgagtatacc atctactcgg ctaggacagc aggagtatcc ttcctccaag tgtaaggcga
      841 aaagtgggga tagtcaagag aagcatggcg accgtaaggg tgcgccacta cgataacgtg
      901 gcggtgttcg ccgctcctgg agttatcgta gtggcgcacc cttacggcat tgtatccaac
      961 taattcattt gtcgagttgc caaaggcggc caaaaggagg acaatccttt cgaaacatct
     1021 catttcctca gaaaggattg tcctccgatg catcatagct tacccgacct gctggcgcaa
     1081 ccccatgcag cggtgccgtt ctttgaaacc gtgctggtgc aagccttgcc tgccgacccg
     1141 cccacgccca agggcagccc gcggggagcg ccccagaagc tctcctggat gcacctgtgg
     1201 ttcggctttg tgttgggcat cctgcaaggg gtggcctcct accagggggt gcagcgtttg
     1261 ctggctgccg accccctggg tccatttgcc aaagtcgtac tcaccgacga tgctctggtc
     1321 aaacgccttc gccaggcggg gattgagccg ctgcagcaga tgctggcccg cctctccgct
     1381 gtgctcgctc cgctcttgcg tcccgccttt cccgccgacc tggccgcctt cgcccgcgag
     1441 atcctggctc tggacgaaac gacctgggaa gccgtgcagc gccacctagc ggtcttgcgg
     1501 gtcgtgccca aaggcgatga gcgcctggtg cctggcaaac tggccgggcg cttcaacgtg
     1561 cgcacgcagc aatgggatct catccaactg cgcaccaacc cggtgggcaa ttgtaaactc
     1621 gacctgtgct ccttgctgga aggcttgctg gccgacagcc tcctgctctt tgatctgggc
     1681 tatttcagtt accccttctt tgattatctg acccaaaggc acttctggtt catctcccgc
     1741 ttgcgcgaga aagtctgtta tcaaatcgtt catgagtttt accgccaggc tggcaccctc
     1801 gatgcgctgg tctggctagg ctccacaggc cgcaacagcc cgcgcaccgg ctgtttgctt
     1861 cgcctggtgc gcttctggga tggccacgag ctgcggatgt atctgaccaa tcagaccgat
     1921 ccgctcgcct tgcccctgcc cgatattgcc cgcctgtacg cccgacgctg ggatatcgaa
     1981 ctggccttct tgacgctcaa agcgcacctg ggcttgcatc actggtggag tgggcatccg
     2041 gtgctccagc agcagcaagc cctggtcgtc ctgatcgccg cccaactgct gcacgccctg
     2101 cgcgtgctca tcgccctgga tgcgggctgt gacccctttg aggtctcgtt gcccttgttg
     2161 gtcgagtacc tgccgcagat cctccatcag cgcctgcatc ccgtcagttg ggccatgcag
     2221 cacggggacg atctcggcct gtttcgtccc tcttctcgtt acgtcgtcca ggctcccgtt
     2281 gtccccacca ctgccctgtg cttccctccc gccgatctgc ctcgtacccg tctggcgtgc
     2341 tatctcacct acaagcagcg cccccatcgg tcgcctcgca agaagccgcg gcgctcgcct
     2401 gcttcctcct ctcgtgcttc tcctcgcctc gacaaatgaa ttagttggag ccaatgaccc
     2461 tt
//
LOCUS       DASZAK010000456         2612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_366770, whole genome shotgun sequence.
ACCESSION   DASZAK010000456 DASZAK010000000
VERSION     DASZAK010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366770"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..2538
                     /locus_tag="VGT44_07935"
     CDS             319..2538
                     /locus_tag="VGT44_07935"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2580769.1"
                     /translation="MQHTARGQQEPQGQPGQFSPSVPEQTTPPPLPVMSQTTPPALRA
                     QRAAGAARFGTTMLTPPVMDGRVILPRVDDVANVLRTLGNAQVSTIVLTGDAGVGKST
                     LAALAFRQLQAAAQAGSSPFSHFIWLGLGTNSSVPDFLAALLSSLRPDALPPDFMQLK
                     PEQQLAYTLQVLRQPQVSALVVIDQFEDLLDIETGLPLPGRGATTLFFQMLKQDLGNS
                     RVLLTCHRSPFGSQNDEESRAKACMISRISLPEGSALLLQRGVVGAPQELSLIWQRCA
                     GNVYGLTLFAALFNLSGFSLSYLLDTPDYQFMWNGDVSLNLVGMVYNFLNPIQRTLLR
                     ALCLFSEPIALAGILAAITGDGQSIDTMAFERELAALTRLALAQYVPGERQHRERREG
                     APVRYFLHARIRQYTIEHYLEGHDRRNSGVLRSAVGVTDEPNAMNANPEAREIALAAG
                     HMRVATYYARQSQAQYLPQEQRGGPLDVAPFLAVMEHLCLGWHWQHAYDLLLEEKLHE
                     RLAQWGAWNTLIRLYTAMIPPAGIVTRSDEAFICSHLGLLYGRLGDYQTGDFYFQQAR
                     ATQREIHDVRGEVTTLINQGELLRGAGQPSQARAIFEQASQLLQSLRTPGVQPDANPN
                     AHADLESALLHNIGLIAQDEKNYDQALHYYLDALKLARGLPESYNLGMILTNTGMLFF
                     EQGRLPEALSLLLQAAQIRQAAQDPTVNTLILFLQALEQKLGPAAYAQLRQEAERLQ"
BASE COUNT          521 a          825 c          774 g          492 t
ORIGIN      
        1 tttgctgtaa gggggctatg ctatacttcc atttgcaagg ggtgaaaaaa aagccaatcg
       61 taggagccga tttatcgcgc tcaccgccga ttgatcggcc agtcattgtc gtcgattaag
      121 tacgtggaca ggcgataatg aattttcacg accaggatcg gtaaccacgc ctgtcatcct
      181 gagcgcagcg aaggatctcg gtagccgacg catcagaccc ttcgctgtgc tcagggtgac
      241 aacaccttgt cgatcctggt tggggtgatt gattgccgtc gatgatatat cgtgggcgat
      301 agggaagata tctagcacat gcagcacaca gcacggggcc agcaggaacc gcaggggcaa
      361 ccggggcagt tttcgccgtc tgtgcctgag caaacgacgc caccgccgtt gcccgtcatg
      421 tcgcaaacga cgccgcctgc gttgcgagcg caacgcgcgg caggcgcagc caggtttggc
      481 accacgatgc tcacgccgcc ggtgatggat gggcgtgtta tcttgccgcg cgtggatgat
      541 gtcgccaatg tgctgcgcac gctgggaaac gcgcaggtca gcacgattgt cctgaccggt
      601 gacgcgggcg tgggcaaatc aacgctcgcg gcgctggcct tccggcaact ccaggcagcg
      661 gcgcaagcag gatcgtcgcc tttcagccat tttatctggc ttggcctcgg cactaactcc
      721 agtgtacccg attttctcgc ggccctgctg agcagcctgc gcccggatgc gttgccgccg
      781 gatttcatgc aattgaagcc agaacaacaa ctggcttaca ccttgcaggt gttgcgccag
      841 ccccaggtca gcgcgctggt agtgatcgat cagttcgagg acctgctcga cattgaaacg
      901 gggctgccat tgcctggacg tggcgcgaca acgctgttct tccagatgct caaacaggat
      961 ctgggcaaca gccgcgtgtt gctgacctgc catcgctcgc ccttcggatc gcagaatgac
     1021 gaggaatcgc gcgcgaaagc ctgcatgatc tcgcgcatca gcctgcccga aggctcggcc
     1081 ttgctgctac agcgcggcgt agtgggcgcg ccgcaggagc tatcgctgat atggcagcgc
     1141 tgcgcgggga acgtctatgg tctgacactc ttcgccgcgc tcttcaatct gagcggcttc
     1201 tccctcagct acctgctcga cacgccggat taccagttta tgtggaacgg cgatgtctcc
     1261 ctgaacctgg tcgggatggt ctacaacttc cttaatccaa ttcagcgcac cctgctgcgc
     1321 gcgctttgcc tgttcagcga acccattgca ctggccggga tactcgccgc cattaccggt
     1381 gacggccaat cgatcgatac aatggccttt gaacgcgaac tcgcggcgct gacgcgcctt
     1441 gcgctcgcgc agtacgtacc tggcgaacgc cagcaccgcg agcggcgaga gggcgcgccc
     1501 gtgcgttatt tcctgcacgc gcgcatccgc cagtacacga ttgagcatta cctggaaggt
     1561 cacgaccggc gcaatagcgg cgtactgcgt tcggctgtgg gagtcacgga cgagccgaat
     1621 gccatgaacg ccaacccgga ggcgcgtgaa atagccctgg cagccggcca catgcgtgtc
     1681 gcaacctact acgcgcgcca gtcacaggcg cagtatctac cgcaggagca gcgcggcggc
     1741 ccgctcgatg tcgcgccgtt tctggccgtc atggaacatc tctgcctggg ctggcactgg
     1801 cagcatgcgt atgatctctt gctcgaagaa aagctgcacg agcggctggc ccaatggggc
     1861 gcatggaata cgctgattcg cctctacacg gcgatgatcc cacccgccgg gatcgtgacg
     1921 cgctccgacg aagccttcat ttgtagccac ctggggctgc tgtatggccg cctgggcgac
     1981 taccagacgg gcgatttcta tttccaacaa gcgagagcca cccagcgcga gatacacgat
     2041 gtgcgcggcg aggtgacgac gctcatcaac cagggcgaac tgctgcgcgg cgcgggccag
     2101 ccctcgcagg cgcgcgcgat cttcgagcaa gccagccagc tgcttcaatc cctccgcacg
     2161 cccggcgtgc agccggatgc caacccgaac gcccacgccg acctggagag tgcgttgctg
     2221 cacaacatcg gcctgatcgc gcaagacgaa aagaactacg atcaggcgct gcactactac
     2281 ctggatgccc tcaagctggc gcgcggcctg cccgaatcct acaaccttgg catgatcctg
     2341 accaacactg gcatgttatt ctttgaacag ggccgcctcc ccgaagcgct ctccctgctc
     2401 ttgcaagccg cgcagatacg ccaggccgcg caagacccca ccgtcaacac cttgatcctc
     2461 ttcctccagg cgcttgagca gaagctagga ccagccgcct acgcgcaatt gaggcaagag
     2521 gccgagcggt tgcaataagc gaaagagctg gcttataaaa tcaatatggc atcggtgtag
     2581 tcagccccag ggcgaccgca agggtctcct tg
//
LOCUS       DASZAK010000457         4644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_437895, whole genome shotgun sequence.
ACCESSION   DASZAK010000457 DASZAK010000000
VERSION     DASZAK010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_437895"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1664)
                     /locus_tag="VGT44_07940"
     CDS             complement(219..1664)
                     /locus_tag="VGT44_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV2580770.1"
                     /translation="MKTFLKNYRWKELGLFVIPFMILLVGMMQLPLAHFYETQLNTTT
                     KAVPFSLAILPTVSALIPIIGLIVVLVIVNVVFSIFFPKADQILLPLVGLMSGIGVLM
                     ATRLQSVAAVGDPNLGTRQLVWVIVGLVVCILTLAGLRRMGWLRRYKYTWAALGILLV
                     AVTLINAFRVRNLNAPTHDQLNFGPLNLQPSELLKIFIVIFFAGFLSDNIDVIRENIF
                     LVGPFRLPLIGRTVTLRLPPLMQLGPIALMLGISLVLFLGIRELGLALLIYGIFLVMI
                     YLGSGKLSYVVVSLIVFFVLGFIGYKLFTYVQDRFTALSIDPTKCQQWGPSCPQQLAQ
                     GSAFQILQGLIALSSGGILGTGIGLGHAGAFVPVVVSDMIFTQIGEELGLAGLFAIIG
                     LYLLILYRGYRIAIEASDSFNQLLAAGLTSIFAIQSLIIILGNMKFLPLTGIPLPFLS
                     YGGSSIIANYIIIGILLRISHITAVEREAAG"
     gene            complement(1689..2135)
                     /locus_tag="VGT44_07945"
     CDS             complement(1689..2135)
                     /locus_tag="VGT44_07945"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2580771.1"
                     /translation="MTLDVFLLILRILLIVLLYLFLMQVVIAISRDLRRSAVAPESNT
                     ANAAPIPGHLVVVDSGPSHIAPGTIFPLKLKSTIGRGPSNTVQIAESFISGEHARMWY
                     HNGSWFVQDAGSTNGTYLNNQPAREALPAHFGDMVQIGFIKFLLKQ"
     gene            complement(2174..3100)
                     /locus_tag="VGT44_07950"
     CDS             complement(2174..3100)
                     /locus_tag="VGT44_07950"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HEV2580772.1"
                     /translation="MKTRQNPLNRVESFMKNLIEGPFAVFFPTKLQPAQVARKLERAM
                     EDASLNQGDGRRIAPEIYDIYLSIKDHQQMQPVEATLKEGWETQLIQFARLHNYYLRV
                     NPVFRLHPRANLRLGDVQIETIMVDRGSNADSGIAHTQQLSKEEVDRLKASLQPGQVL
                     PGITGASGMMTNPSTGLPQQGQYSYPGGRPPMSGGSGMLPMANGYAPAMPQAWLTIKL
                     PQSEVRKEYRMDKAVVGIGRQLDNDIIVEDKRVSRYHAQIKYLPDGQFIIYDLDSING
                     INVNGVPNQRQHVLQNGDRFTIGSYDFYFQRR"
     gene            complement(3097..4077)
                     /locus_tag="VGT44_07955"
     CDS             complement(3097..4077)
                     /locus_tag="VGT44_07955"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV2580773.1"
                     /translation="MELTTTLSPEQPIAITIGNFDGIHRGHQRLLYELGEAARETGSA
                     PVMVTFDPHTLLVVRPDIKLECLTTLEEKLLLARRYGGVSGTIVVHFTKELAGVSAAE
                     FLDELRARFAIRALVVGSNFSFGHNRQGNVTFLRQYGQEHGIVVRAIELEEAERARIS
                     STRIRSLVSEGQIIEANELLGHPVITNGIVVRGDQRGRQLGFPTANLVPQPQKLLPAN
                     GIYAARVYVGEMAKSDGSVNVAVVYDEARTVWKSAVSVGVRPHFDGQKRLVEAYLLDT
                     ELDLYGRLIIIEFVARLRGEQRFNSLDELIAQIASDVEQTRQLLSEEIKG"
     gene            complement(4149..>4644)
                     /locus_tag="VGT44_07960"
     CDS             complement(4149..>4644)
                     /locus_tag="VGT44_07960"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:NF027523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV2580774.1"
                     /translation="AGEVLTMEARPITITRLEIIDWQPPALILDIECSKGTYIRSLAH
                     DLGERSGYLAHLQALVRTRSGPFRLDDSITLEQLADALIRGEAEPYLRPADMALEHYP
                     ALTLDEETATRVRHGNAFEGYNAAVSIKEQKLARVYDSDGRFVAIAAWDDARQVWQPV
                     KVFE"
BASE COUNT         1057 a         1292 c         1240 g         1055 t
ORIGIN      
        1 ccagctggct ctcaagctgg gccagttctt cttcgagcgc ccggcgcgtt ttacgcaggc
       61 tccgttctac atcctgatat cgtaaccact gctggtacaa acccatgtgt cctattccta
      121 cacatcgtct tacatgtatt ctctcacgtt gcatcatttc tgatgatgga agtatagcat
      181 ctccatggta gagagacaag caagcggacg gtgttatctt atcctgccgc ttcacgttcg
      241 acggcggtga tatgcgagat gcgcagcagg atgccgataa tgatgtagtt ggcgatgatg
      301 gaactaccgc catagctgag gaagggcaac ggaatacccg tcagtggcag gaacttcata
      361 ttgcctaaga tgatgatgag cgattggatg gcgaagatgg aggtcaggcc ggcggcgagc
      421 agttgattga aggagtcgct agcctcgatt gcgatgcgat agccccggta gagtatcagc
      481 aggtagaggc cgataatagc gaacagcccg gccagcccca gttcttcgcc tatctgggtg
      541 aagatcatat cggacaccac taccggcacg aatgccccgg cgtgtcccag gccgatgccg
      601 gtgccaagaa tacccccgct cgagagggcg atcagtccct gcaaaatctg gaaggcggaa
      661 ccctgggcaa gttgttgagg gcacgagggt ccccattgct gacatttcgt tggatcgata
      721 gagagagccg taaaacgatc ttgcacatag gtaaatagct tatagccgat aaagccaagc
      781 acgaagaaga cgatcaggct gacgacgaca tacgagagtt tgccgctgcc caggtaaatc
      841 atcaccagga agataccgta gatgagcaag gccagtccaa gttctcgaat accgaggaac
      901 aagaccagcg agatgccgag catgagcgcg atcggcccaa gctgcatcag cggcggcagg
      961 cgcagcgtga ccgttctgcc gatgagcggg aggcggaaag ggccgaccag aaagatattt
     1021 tctctgatga cgtcgatatt atcactgaga aagcctgcga aaaagatcac gataaagatt
     1081 ttcaacaact cggatggctg gagattgagt gggccaaagt ttaactggtc gtgagtcgga
     1141 gcattcaggt ttcgcacacg aaaggcattg atcaatgtga ccgccaccag gaggataccg
     1201 agcgcggccc aggtgtattt gtagcgtcgc agccagccca tcctgcgcag cccggccagc
     1261 gtcagaatgc agacgaccag cccgacgatc acccatacga gctggcgcgt ccccaggttt
     1321 ggatcgccaa cggctgccac gctttgcagg cgagtcgcca tcaggacgcc aatcccgctc
     1381 atcagaccca cgaggggcag cagaatctgg tcggctttag gaaaaaatat gctgaagaca
     1441 acgttgacga tcaccagcac gacgatcagg ccgatgatgg ggattaaggc gctgacggtc
     1501 ggcagaatgg ccagcgaaaa cggcaccgct ttcgtcgttg tattgagctg cgtttcataa
     1561 aaatgggcaa ggggcagctg catcatgccc acgagcagaa tcatgaatgg gatgacgaac
     1621 agtcctaatt ctttccatcg atagtttttc agaaaagttt tcatctacat gcccaatctc
     1681 tgagaatctt attgtttcaa aagaaattta ataaacccga tctgcaccat atcgccgaag
     1741 tgagccggga gggcttcacg cgcgggctgg ttattcaggt acgtgccgtt ggtgctgcca
     1801 gcgtcctgca caaaccagga gccgttgtgg taccacatac gcgcatgttc gccggagatg
     1861 aaactttccg cgatctggac agtgttcgat ggacctcgtc caatagtact cttcaatttc
     1921 aacggaaaga tggtccccgg cgcgatgtgg ctcggtccac tgtccaccac gaccaggtgt
     1981 cccggaatag gcgctgcatt cgccgtgttc gactccggtg cgacagccga tctcctcaga
     2041 tcgcgcgaga tggcgatgac gacttgcatc aaaaagaggt agagcaaaac aatcaataga
     2101 atgcgtaaga ttaataaaaa aacatctaaa gtcatacact tttttcaggg tttcctgtgc
     2161 ggcaacccgc cccttatctt ctctgaaagt aaaaatcata actgccgatg gtgaaccggt
     2221 cgccattttg caacacatgt tgtcgttggt ttggcacacc attcacattg atgccattga
     2281 tactatcgag atcatagatg atgaattgcc cgtcgggcag gtatttgatt tgggcgtgat
     2341 agcgagagac acgcttgtct tcaacgataa tgtcattatc aagctgcctg ccgatgccga
     2401 caaccgcttt atccatacga tattctttcc tgacttcgga ctggggcagc ttgatggtga
     2461 gccatgcctg cggcatcgca ggcgcgtagc cattcgccat cgggagcatc ccggagccgc
     2521 cgctcatcgg tgggcgccct cccggatagc tgtactgtcc ctgctgaggt aatcccgttg
     2581 aaggattggt catcatacct gaagcgccgg taatgcccgg cagcacctgc cctggttgca
     2641 ggcttgcttt taagcgatcc acttcttctt tgctgagctg ctgcgtgtgc gcgatgccgc
     2701 tatcagcatt cgatccccgg tccaccatta tcgtctcaat ctgtacatcg cccaggcgca
     2761 gattggcgcg agggtgcagg cgaaagacgg ggttcacgcg caggtaataa ttgtgcaggc
     2821 gcgcgaattg gatgagctgc gtttcccatc cctctttcaa tgtggcctcg acgggctgca
     2881 tttgctggtg atcttttatg ctgagataaa tgtcgtaaat ctcaggagca atgcgcctgc
     2941 catcaccctg gttcagcgag gcatcctcca tagcgcgttc aagtttgcgg gccacctgcg
     3001 cgggctgcag tttggtgggg aaaaaaaccg caaacggccc ctcaatcagg ttcttcatga
     3061 atgattcgac tctgttgagt gggttctggc gagttttcac cctttgattt cctctgataa
     3121 aagctgccgc gtttgctcta catcgctggc aatttgcgcg attaattcat caagactgtt
     3181 aaaacgttgc tcaccgcgca agcgcgctac aaactctatt ataatcaacc tgccatacag
     3241 gtcaagttcc gtatccaaca ggtatgcctc gaccaggcgt ttctggccat caaagtgtgg
     3301 gcgtactcca acactgaccg cactcttcca gacggtgcgg gcttcatcat acacaacggc
     3361 tacattcacg ctcccgtcac ttttggccat ttcgcccaca tatacccgcg cggcatagat
     3421 accgttggcc ggaagcagtt tctgtggctg tgggaccagg ttcgctgttg gaaagcccaa
     3481 ttgcctgcca cgctggtcgc cccggacgac gatgccattg gtaatcacgg gatgtcccag
     3541 cagttcgttt gcctcgataa tctgcccctc gcttactaaa gaacgtatgc gcgtgctgct
     3601 tatacgcgct cgttccgcct cttccagttc aatcgcccgc accactatgc cgtgttcctg
     3661 cccgtactgc cgcagaaagg tgacattgcc ctggcggtta tgtccaaaac tgaaattgct
     3721 gcccaccacc agcgcccgga tagcgaagcg agcgcgcagt tcgtccagaa actctgcggc
     3781 gctcacccct gccagttcct ttgtaaaatg tacaacaatc gtgccactca ctcccccata
     3841 ccgccgggcc agcaaaagct tctcttccag cgtcgtcagg cattcaagct tgatgtccgg
     3901 cctcaccacc agcagcgtgt ggggatcgaa tgtcaccatc accggcgcgc ttcccgtctc
     3961 tcgcgccgcc tcgcccagtt cgtacagcag ccgctgatgc ccccggtgaa tcccatcaaa
     4021 attgccaatg gtaatcgcaa tcggttgctc aggcgataaa gttgttgtta attccatact
     4081 ttgtttgaat gactatcctc caggttctca ggtgtatagt atcacagcgg gggagagaag
     4141 taaacgaact attcaaatac cttcactggc tgccatacct gtcgcgcatc atcccatgcc
     4201 gcaatagcca cgaatcgtcc gtcgctatca tacaccctgg ccagcttttg ctcctttata
     4261 ctcactgctg cgttgtatcc ctcaaacgca ttcccgtgcc gcacccgcgt tgccgtctcc
     4321 tcatcgagcg tgagggcggg ataatgctcc aaagccatat ctgccggtcg aaggtatggc
     4381 tcagcctcac ctctgatgag agcatccgcg agttgttcga gggtgatgct gtcgtccagg
     4441 cgaaatgggc cgctgcgcgt gcgcaccagg gcttgcaggt gcgccaggta gccggagcgt
     4501 tcgccgaggt cgtgcgccag cgagcggatg taggtgcctt tgctgcattc gatgtcgagg
     4561 atcagcgcgg gcggttgcca atcgatgatc tccagtcggg taatcgtgat ggggcgtgcc
     4621 tccatggtta gcacttcgcc ggcc
//
LOCUS       DASZAK010000458         8143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_45779, whole genome shotgun sequence.
ACCESSION   DASZAK010000458 DASZAK010000000
VERSION     DASZAK010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8143
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45779"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VGT44_07965"
     CDS             <1..931
                     /locus_tag="VGT44_07965"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HEV2580775.1"
                     /translation="AGAEWRDVMESVRAPLRRVLQAQLERCKRKAAALQQELAISEEA
                     SAYRLWGDLLLAHQHEIGQGQSSVELQNYFSGEEGAPPVTVPLDPRFDAIGNANRLFH
                     KYHKLRRALELVPSQIEQNAAELATVEQLQADLVLAETPAEAALVKAEVQEAGYLRGK
                     KAADARKTFQKGGKGGKKGGGKDKKGVPPGGGVPLHVQSRDGFTILIGKNSRQNEEVT
                     FRQAAANDVWLHARGVPGAHVIIKAGGRDIPRSAIAQAAGLAAYYSQARGSTSVPVDY
                     TLQRHVRHMKGGGPGMVIYDRERTLYAEPSATS"
     gene            1161..2516
                     /locus_tag="VGT44_07970"
     CDS             1161..2516
                     /locus_tag="VGT44_07970"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEV2580776.1"
                     /translation="MQSAIVIADDIGSQLSHWIFSGIFLQFLVAFLFAFIFPITSAAI
                     INYLERKVMAWMQDRKGPLHVGPHGSLQLLADVGKMFTKEDIHAAQTDKAVFLLAPSV
                     FIAPMVASFAVLPFSPFIGLPGTALATGIVYLVAMSSLDVIGVVMAGWGSNNKYSLIG
                     GLRAAAQMISYELPLVLALVGVVILTSVLAGSPGYPGNAGIGTISLKEIMTFQSTWKD
                     TGIGILDFIFKGFTPWAWFILVQPLMLLIYYICGLAETNRAPFDLPEAESELVAGFLT
                     EYSGVRWAMFFLGEYGNMTIVSAITTSLFLGGWSGPGVGFLTASGMVFGWQLLGNVLG
                     VIYFVAKVYLLCVVFIWVRSTLPRLRADQLMQFAWLMLIPATLGNILLTGLIYLVISG
                     LGLSNLVFLIVTGAINWLLLIGFIRLVGRAIVVTTRRAQAPALHARARQAALAQSTID
                     V"
     gene            complement(2548..3996)
                     /gene="rpoN"
                     /locus_tag="VGT44_07975"
     CDS             complement(2548..3996)
                     /gene="rpoN"
                     /locus_tag="VGT44_07975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEV2580777.1"
                     /translation="MRLDPTPRPEQTLRVSARLITASNILHLSSDELERAVAQEQMEN
                     PALEVIEQRICLFCGSPLRGPVCASCGASAQQAQPAFNAVEHIQVDEGAAEQQWNDYA
                     AFDIYDYSAATDSDEEEELDPMARLAMDQSLAETLLQQLESLVEPDELPIAEQLVGNL
                     NERGYLEIGVSDIAAYLEVPVDRVERVLSLLQTLDPPGIGARDARECLLIQLAILHEH
                     ETPHPLAAPLIDRYLDRLGKSQFHEIARELKVPEHNVRQAAQYIRSNLYPFPAHTYQP
                     GATGGQGAIYTRPDVIIRKGEHGFEVELIEEKRYGFHIGPGYGTQPLSLEHDPAFDEM
                     QRYMRAHSDRAKFFIDCILRRWRTLKLVTELIVDYQREFLEKGVRFLRPFTRAEVAAR
                     LNLDESTVSRATAHKYAMLPNGRLMPLADFFDGSLGIKDVLRELITAEDPKHRLSDDE
                     LARLMTARGIPMARRTVTKYREEMGIASSRER"
     gene            complement(4012..4713)
                     /gene="rnc"
                     /locus_tag="VGT44_07980"
     CDS             complement(4012..4713)
                     /gene="rnc"
                     /locus_tag="VGT44_07980"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV2580778.1"
                     /translation="MFEAMEAALGVHFHDTDLLKLALTHSSYIYETPGGTDLQSNERL
                     EFLGDAILNCICADYLYRAFPALSEGDLSDVRAILVKGETLAQFASEIDIGPHLVMSK
                     GEAKGNKSQRVLAAAFEAVLGAIFLDQGLDETRRFLLPRLEPMAQHIVQIHLFKDSKS
                     LFQELAQAHDGITPSYRLVGQEGPSHNREFTVEVMLGDKVAGRGLGRNKKLAEQAAAR
                     AALQQRGWLNGKDMG"
     gene            complement(4713..5771)
                     /gene="selD"
                     /locus_tag="VGT44_07985"
     CDS             complement(4713..5771)
                     /gene="selD"
                     /locus_tag="VGT44_07985"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5718..5720),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEV2580779.1"
                     /translation="MSTGRRIRLTSLASCAGUAAKMGPEALEQVLRPLALQSAPPALL
                     VGLHSADDAAVYQVNEQQAIISTADFFPPVVDDPYAFGAIAAANALSDIYAMGGQPLM
                     AINLVAWPADLEPALLSEILRGGADIVAKAGAVIAGGHTVTDAEPKYGLSVTGMVHPQ
                     HILTKGGAKTGDSLVLSKPLGTGILTTAHKQDVVGTADLDAALQSMLRLNDKASQALR
                     YLPHAGVHAVTDITGFGLLGHAWEMASQSGVGMRFSFDTLPLLPRVRHYIEQDCITGG
                     ARRNEQYLKKHVTFHDQLGRVEREILWDPQTSGGLFAAIDPALWPQLAALTLQDDGVQ
                     FWRIGDVVEAEQATLEVM"
     gene            5914..6315
                     /gene="rnpB"
                     /locus_tag="VGT44_07990"
     ncRNA           5914..6315
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGT44_07990"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(6353..8080)
                     /locus_tag="VGT44_07995"
     CDS             complement(6353..8080)
                     /locus_tag="VGT44_07995"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV2580780.1"
                     /translation="MPVFQITSTGPQLATPPKKRARRLPYQWYILARALTGGLIGGLS
                     FTLLYNILGHPESFFILLPISVSLAWAQPLRYNLGKFLSSTPIYLLLTICWTFIYYGA
                     LAESQVLLYQDPTIPHIIPIASSISWAIIFDPVRAYVQQHIERRFNFRDQEAAKAIET
                     FTSTLREEIDLDQLRERFLTVIHRTMQPYSVSLWIRTSRAQRAQSGSTEEIGVADDDP
                     LIAYVLRHPVTLEVDRLRLDSPVLQDLKLHAADILMPLASQGELVGLLIMGPHLKGER
                     YAHEELTLLDALAPQVAPALRVALLVQEQKEQVRVRERIEQEQVRERQRIEQEQARER
                     QRIEQELQTARSIQHAFLPRDVPALPGWQFAPYYQPAREVGGDFYDFLPFADGRLGLV
                     IGDVTGKGIPAALVMATVHTMLRTAVQGIISPGEVLARVNALLATDIPPGMFVTCFFA
                     LLDPQSGQLHYANAGHEPPYRQHNGSAIELWATGMPLGMLPGTHYEEYEATLVPGESL
                     LFYSDGLVEAHNPGHEMFGFPRLQKLLAEHTDGMPLIDVLLDELKSFTGAEWEQEDDV
                     TMLTLQRTS"
BASE COUNT         1585 a         2384 c         2470 g         1704 t
ORIGIN      
        1 cgcgggcgcg gaatggcgcg acgtgatgga gagtgtacgc gcaccgctgc gccgggtctt
       61 gcaagcgcaa ctcgaacgct gcaagcggaa agccgccgcg ctgcaacagg aactggccat
      121 ctcggaggag gccagcgcct accggctgtg gggcgacctc ctgctggctc accagcacga
      181 gatcgggcag gggcaatcca gcgtcgagct gcaaaactat ttttccggcg aagagggcgc
      241 gccgccggtg acggtgccgc tcgatccgcg cttcgacgcc atcggcaacg caaaccggct
      301 cttccataaa taccacaagc tgcgccgcgc cctggaactc gtcccttcgc agatcgagca
      361 aaacgccgcc gagctggcca ccgtcgagca gttgcaggcc gacctcgtcc tggctgagac
      421 gccagccgaa gccgctttgg tcaaggccga ggtgcaggaa gccggttact tgcgcggcaa
      481 gaaagcagcc gatgcgcgca agacgtttca aaaaggcggc aaaggtggta agaagggagg
      541 ggggaaagat aaaaagggtg tgccgcccgg cggtggcgtg ccgctgcatg tgcagagtcg
      601 cgacggcttc acgatcctga ttggcaagaa cagccgtcag aacgaagagg tcacgttccg
      661 ccaggctgcc gcgaacgatg tatggctgca cgcgcgcggc gttcccggcg cgcacgtcat
      721 catcaaagcc ggtggccgcg acatcccgcg cagcgccatc gcgcaggccg ccggcctcgc
      781 cgcctattac agccaggcgc gcggcagcac cagcgtcccg gtagattaca ctctgcaacg
      841 ccacgtgcgc cacatgaagg gcggcggccc tggcatggtg atctacgacc gcgaacgaac
      901 gctgtacgcg gaaccatccg caacctcgta attgagtgtc atcctgagcg cagcgaagga
      961 tctcggcctg cggcgagtga ggcccttcgc tgcgctcagg atgacaagac cgatcttggt
     1021 cacgaaaatt catcatcgcg tccctacgac cggggagcgc atcatcgcag agcggtggta
     1081 tactactagt agctcatgtt ttcttcagac gacctcatgg agtggttcgc atgtcatgct
     1141 gcgagaatgg gagctagctc atgcagagcg caatcgtaat cgctgacgat attggcagtc
     1201 agctctctca ctggattttc tcagggatat ttttgcagtt cctcgtcgca tttctgtttg
     1261 cctttatctt tcccatcacg agtgcagcca tcatcaacta cctggagcgc aaggtcatgg
     1321 cgtggatgca ggatcgcaag gggccgttgc atgtaggccc gcatgggagc ttgcagttgc
     1381 tggccgatgt cggaaaaatg tttacgaaag aagacattca tgccgctcaa acggataaag
     1441 ccgtcttctt gcttgcgccg tcggttttta ttgcgccgat ggtcgcctcg ttcgcggtgc
     1501 tgcctttctc gcccttcatc ggcctgcctg ggacggcgct ggcaaccggc atcgtctacc
     1561 tggtggcgat gagttcgctt gacgtgatcg gtgtagtgat ggcgggctgg ggatcgaaca
     1621 acaagtactc gctgatcggt ggcctgcggg ctgccgcgca gatgatctcc tacgaattac
     1681 cactggtgct ggccctcgtg ggcgtggtga tcctgacgag cgtgctggcc ggctcgcccg
     1741 gctatcctgg gaacgcgggc atcggcacta tctcgctcaa agagattatg accttccaga
     1801 gtacctggaa ggacaccggc atcggcatcc tggacttcat cttcaaaggc tttacgccgt
     1861 gggcctggtt tatcctggtg cagccgttga tgctcctcat ctattacatc tgcggccttg
     1921 cggagacgaa ccgcgctcca ttcgatctgc cggaggccga atcggaactg gtcgccggtt
     1981 ttctgaccga atacagcggc gtgcgctggg ccatgttttt tctcggtgaa tacggcaaca
     2041 tgaccatcgt ctccgccatc actacctcgt tgttcctggg cggctggtct ggccccggcg
     2101 ttggcttcct gacggcctcg ggcatggttt tcggatggca actgcttggg aatgtgctgg
     2161 gagtaatcta ctttgtggcc aaggtctacc tgctgtgtgt cgtcttcatc tgggtgcgca
     2221 gcacacttcc ccgcctgcgc gccgatcaat tgatgcaatt tgcctggtta atgctcatcc
     2281 cggctaccct gggcaatatc ctgctcaccg gcctgattta cctcgtgata agtgggcttg
     2341 gcctgtcaaa cctcgtcttc ctgatcgtga cgggcgcgat caactggctg ctgctgatcg
     2401 gcttcattcg actggtgggg cgcgcgattg tcgtcacgac gcgccgggcg caggcacccg
     2461 ctctacacgc ccgcgcgcgt caagccgccc tcgcgcaatc gacgatagat gtgtaggggc
     2521 ggtcaataat caaaatccct ggcatcatca ccgctctcgc gaggaggcaa tgcccatctc
     2581 ctcgcggtac ttcgtgacgg tgcggcgggc catggggatg ccgcgcgcgg tcatcaggcg
     2641 cgccagttcg tcgtcgctca agcggtgttt ggggtcttcg gcggtgatca gctcgcgcag
     2701 cacatccttg atacctagcg agccgtcaaa gaaatcggcc aggggcatca ggcggccatt
     2761 gggcagcatg gcgtatttgt gcgcggtggc gcggctgacg gtgctttcgt ccaggttgag
     2821 gcgcgcggcc acctcggcgc gggtgaaggg acgcaagaag cggacgccct tctcgagaaa
     2881 ttcgcgctgg taatcgacga tcagttccgt gaccagcttg agcgtgcgcc agcggcggag
     2941 aatgcaatcg atgaagaatt tggcgcggtc gctgtgcgcg cgcatgtagc gctgcatctc
     3001 gtcgaaagcc gggtcgtgtt ccaggcttaa cggctgcgta ccgtagccgg gaccgatgtg
     3061 gaagccgtag cgtttctctt cgatcagttc gacctcgaat ccatgctccc ccttgcggat
     3121 aatcacgtca gggcgcgtat agattgctcc ctggccgcca gtggctcccg gctggtaggt
     3181 gtgcgccggg aagggataca ggttgctgcg gatatattgc gcggcctggc gcacattatg
     3241 ttcgggtacc ttcaactcgc gcgcgatctc gtggaactgg cttttgccca accggtcgag
     3301 gtagcggtcg atcaatgggg cagccagggg gtgcggcgtc tcgtgttcgt gcaggatggc
     3361 cagttggatc agcaggcact cgcgcgcgtc ccttgcccca atgcccggcg ggtccagcgt
     3421 ctgtaacagg ctcagtaccc gctcgacgcg atcgacagga acttccagat aggccgcgat
     3481 atcgcttacc ccaatttcga gatagccgcg ttcgttgaga ttgccgacga gttgttccgc
     3541 gatgggcaac tcgtctggct caaccaggct ctccaattgt tgcagcagcg tttcagccag
     3601 cgactgatcc atagccagcc gagccatcgg gtcaagctct tcctcttcgt cgctgtcggt
     3661 cgcggctgag tagtcgtaga tgtcgaacgc cgcgtaatcg ttccattgtt gttcagcggc
     3721 cccctcgtcc acctggatgt gttcaacggc gttgaacgct ggctgggctt gctgggcgct
     3781 ggctccgcat gaggcgcaga ccggcccgcg caggggcgat ccgcagaaca ggcaaatgcg
     3841 ctgctcgatc acttcgaggg cgggattctc catctgttcc tgggcgacgg ctcgctccaa
     3901 ttcatctgat gagaggtgta atatgttgct cgcggtaatc agccgcgcgc tgacccgcag
     3961 tgtctgttct ggtcttggtg ttgggtccag ccgcataact tcccccctga cttaccccat
     4021 atctttacca ttcagccatc ctcgttgttg cagtgccgcg cgcgccgccg cctgctcggc
     4081 cagctttttg ttgcggccaa gtcccctgcc tgctaccttg tcgcccagca tcacctcgac
     4141 ggtgaactcg cggttatgtg atggtccttc ctggccgacc agtcggtatg atggtgtgat
     4201 gccgtcatgc gcctgcgcca gttcctggaa aagcgatttg ctgtccttga aaagatgtat
     4261 ctggacaata tgctgcgcca tcggttccag gcgaggcagc agaaagcgcc gcgtctcgtc
     4321 aagcccctgg tcgaggaaga tggcccccag cacggcctcg aacgcggcag ccagcacgcg
     4381 ctggctctta ttgcccttcg cctcgccctt gctcatcaca aggtgcgggc cgatgtcgat
     4441 ctcgctggcg aactgtgcta gcgtttcgcc tttcaccagg atggcgcgca catcactcag
     4501 atccccctcg ctcaatgcgg gaaaggcgcg atagaggtag tccgcgcaga tacagttgag
     4561 aatagcgtcg cccaggaatt cgagccgctc gttggattgc aagtccgtgc ctccgggtgt
     4621 ctcgtagata tacgagctgt gcgtcagcgc cagtttaagc aaatccgtat cgtggaaatg
     4681 cacacccagc gccgcttcca tcgcctcgaa catcacatta cctccaaagt tgcctgctct
     4741 gcctctacga catcgccgat acgccagaac tgcaccccat catcctgcaa ggtcagcgcg
     4801 gccagttgcg gccagagcgc cgggtcaatg gctgcgaaga gaccgccgga cgtttgtggg
     4861 tcccacagga tttcacgctc gacgcgcccc aactgatcgt gaaaggtcac gtgcttcttg
     4921 agatactgct cgttgcgccg cgcgccgccc gtgatgcagt cctgttcgat gtagtggcgc
     4981 acacgcggca atagcggcag tgtgtcaaag ctgaagcgca tcccaacgcc gctttgcgag
     5041 gccatctccc aggcatgacc gagcaggccg aagccggtga tgtctgtaac agcatgaaca
     5101 ccagcatgcg gtaagtatcg tagcgcctgg ctggccttat cgttcaggcg caacatcgat
     5161 tgcagcgccg cgtccaggtc ggctgttcct acaacatcct gtttgtgcgc ggtggtgagt
     5221 atgccggtgc caagcggctt gctcaataca agggagtcgc ccgtcttcgc tccgcctttt
     5281 gtgaggatgt gttgtgggtg aaccatgccg gtcacggaca gcccatattt cggttccgcg
     5341 tcggtcacag tatgcccccc ggcgatgacc gcgccagcct tcgcgacgat atcagcgccg
     5401 ccgcgcagga tttcgctcag caaagctggt tccaggtcgg caggccaggc caccaggtta
     5461 atagccatga gcggctgacc gcccatcgcg tagatgtcgc tcaacgcatt ggcggctgca
     5521 atcgcgccga aggcatacgg atcatccacg acgggcggga aaaagtccgc cgtactgatg
     5581 atggcctgtt gctcgttcac ctggtagacc gcggcatcgt ctgccgagtg cagtccaacc
     5641 aggagagcgg gcggcgcgct ttgaagggcg agtggacgca gaacctgctc cagggcctcc
     5701 ggccccatct tggccgctca gccagcgcag cttgccagcg atgtcaggcg aatccgccga
     5761 cctgtggaca ttgggatacc ctttgctaga cggttatcgt tccagtatgc cgaaccgtga
     5821 cggcaactgc accgtcattg tagcgcgtag gtggtcggcg cgtcaatcgg ctggacaacc
     5881 taccggttct atgttaccct agttgcgcgg gtttgagtag cacaggcaat cgcgggccgc
     5941 gaggttcgag gaaagtccga actccacaga gcgggatgct ggataacgtc cagtgagagc
     6001 gatctcaagg aaagtgccac agaaacatac cgcccggcac ccagacgaat acaggccgct
     6061 tgccgggtaa gggcgaaatg gtgaggtaag agctcaccag cagccgggcg accggctggc
     6121 tgggtaaacc ccatccggag caagaccgaa cagaaggagc agcgcgccgc gaggcgcgct
     6181 cgcgggttgc tcgtccgtta accttcgggt aggtcgcacg aggcgcgtgg taacgcgcgc
     6241 cctagataga tgattgccac ctgcctgtcg cgagatgagg cagggacaga attcggctta
     6301 tgggctgctc acacccgcga cccacgccat tttcctgata atgcagaaaa gtttatgatg
     6361 ttctttggag cgtgagcatt gtcacatcat cttcttgctc ccactctgcg ccggtgaagc
     6421 tcttcagttc gtccagcaaa acgtcaatga gcggcattcc atcggtatgt tctgcgagca
     6481 gcttttgcag gcgcggaaaa ccaaacatct catgtccagg gttatgagcc tccaccaggc
     6541 catcgctata gaaaagcagg ctttctcccg gcactagcgt cgcttcgtac tcctcgtagt
     6601 gtgtgccggg cagcattcca agcggcatgc cggtcgccca gagttcaatg gcgctcccat
     6661 tgtgttgacg gtatggaggt tcgtgccccg cattcgcgta gtggagttgc ccgctctggg
     6721 gatcaagcag cgcaaagaag cacgtgacaa acattccagg tggtatatca gtggcgagca
     6781 atgcgttgac tcgcgctaag acctcgcctg gagagatgat cccctgcaca gcagtgcgca
     6841 acatcgtatg cactgtcgcc atcaccagcg cggccggtat gccctttccg gtcacatcac
     6901 cgatgacaag ccccaaacgc ccatcagcaa agggcaggaa atcgtagaaa tcgccgccta
     6961 cttcgcgagc aggttggtaa tatggggcga actgccaacc ggggagggca ggtacgtctc
     7021 tgggcaaaaa ggcgtgctgg atagagcggg cggtctgtag ttcttgctcg atgcgctggc
     7081 gctcgcgcgc ctgttcctgt tcgatgcgtt ggcgctcgcg cacctgttcc tgttcgatgc
     7141 gttcgcgcac acgtacctgt tccttttgtt cttgtaccag aagcgccacg cgcaaggccg
     7201 gcgcaacctg gggagcaagg gcatcgagta gtgtaagctc ctcgtgggca tatcgctctc
     7261 ctttcagatg cggccccatg atgagcagac cgaccagctc tccctggctg gctaagggca
     7321 tgagaatatc cgccgcatga agcttcaggt cttgtaaaac cggcgaatcc aggcgcagac
     7381 ggtctacctc tagagtaacg gggtgacgca ggacataggc aatcagagga tcatcgtcgg
     7441 cgaccccgat ctcttccgtc gagccggact gtgcccgctg agcgcgagaa gtgcgtatcc
     7501 ataaagagac agagtacggc tgcatcgttc gatggatgac ggtcaagaag cgctcgcgca
     7561 attgatcgag gtcaatctcc tcgcgcagcg tcgaagtgaa tgtctcgata gctttggctg
     7621 cctcctgatc ccgaaagttg aagcgtcgct caatatgttg ctggacatac gcgcgcacag
     7681 gatcgaatat aatcgcccag gaaattgagg aagcgatagg gatgatatga ggaatggtag
     7741 ggtcctggta gagaagcact tgcgattcgg cgagcgcacc ataatagatg aacgtccagc
     7801 aaatggtgag caagaggtaa attggcgtgc tactgagaaa cttaccaaga ttgtagcgca
     7861 ggggttgtgc ccaggcgagg ctaacggata tgggaagtaa gatgaagaaa ctttcaggat
     7921 gtcccaggat attatacaat aacgtgaatg aaaggccacc gattagtcct ccagttaaag
     7981 cgcgcgcaag gatataccac tggtagggaa ggcgtcgagc gcgcttcttc ggtggggtag
     8041 ccagttgtgg gccagtcgat gttatctgga atacgggcat cgttcgcttt tcctttctac
     8101 caccgtttca tcatatctgc tgtgttcatc attgacaggc gta
//
LOCUS       DASZAK010000459         4051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_543361, whole genome shotgun sequence.
ACCESSION   DASZAK010000459 DASZAK010000000
VERSION     DASZAK010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543361"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="VGT44_08000"
     CDS             complement(<1..1176)
                     /locus_tag="VGT44_08000"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2580781.1"
                     /translation="MTDRVGQQLGNYRLIRSLGKGGFGEVYLATHIFLKTPAAIKVLH
                     TQVVDADLDSFLQEAQTIASLDHPSIVRVLDFGIEGATPYLVMSFAANGTLRQRHPTG
                     TRLPFNTALSYARQVAAALHYAHTRKLIHRDIKPENMLLGANNEVLLSDFGIALVAQS
                     SRLQSTQEVVGTVAYMAPEQLQGKPRPASDQYSLGIVLYEWLSGERPFHGTFVELYSQ
                     HMFVPPPSLSQKLPTVPLDIEQVMMRALAKDPQQRFESVTAFVTALEEASSPTQRSDY
                     TPTRPAAFPARPQAAKTLYPPTQQATPQLEWPTITRQASEPLTPLPEAVSDPQLSSNP
                     TLEATPRGILPPQMPFPPVQVRSQTIPNGPVSTPGIFPSDPGGETPQIRGVSRRLMLA
                     "
     gene            complement(1294..2127)
                     /locus_tag="VGT44_08005"
     CDS             complement(1294..2127)
                     /locus_tag="VGT44_08005"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEV2580782.1"
                     /translation="MTCEEFEELSGAYVLDAVTPEERQAAQEHLATCDKCTRLAQELR
                     AAVSVLPYAAPQVTPSPALLERIIQAIRQKPRPVATPPPRRVRQPTRRRAGWATRILA
                     VAALVLFLITGGMTAWNVSLQHQLTTVQASNTQLTGQLSALQQSNAAQARQIGALNRQ
                     VAQVFNIAGTPAAHTASGSLYYLPQQNITVLVLHNLPALQGSHIYQGWLLHKGAPTSI
                     GVLSEQNGIASLIFPGAIGNYDTAAVSQEAGPGPTPRAPAGKVVAAGLLQHPTQAIFT
                     I"
     gene            complement(2276..2361)
                     /locus_tag="VGT44_08010"
     tRNA            complement(2276..2361)
                     /locus_tag="VGT44_08010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2325..2327),aa:Ser,seq:gga)
     gene            complement(2373..2457)
                     /locus_tag="VGT44_08015"
     tRNA            complement(2373..2457)
                     /locus_tag="VGT44_08015"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2421..2423),aa:Ser,seq:cga)
     gene            complement(2539..2829)
                     /locus_tag="VGT44_08020"
     CDS             complement(2539..2829)
                     /locus_tag="VGT44_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580783.1"
                     /translation="MKNNSPSPQSSEHSERVERPDHSVRQADLNDPIGENSEQAQMDA
                     LMQRGFVWDEAVKLFYLRDHLYSNGEMQQRIANDHHMQFMRWLYEQGELQES"
     gene            3063..3911
                     /locus_tag="VGT44_08025"
     CDS             3063..3911
                     /locus_tag="VGT44_08025"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HEV2580784.1"
                     /translation="MSAFATNEDVWAEFVTTRHPAFRDELILIYMPLVRFVVSRLGIP
                     SVGVLDQEDLVSFGVIGLINAIDRFEPARGIRFEAFATPRIRGAVIDQLRAINWFPRS
                     AIKRIRQVEGALAELEQRLGRSPTEVEVATEMDVSVERYRHMMQEANTTIFSLDAPLS
                     SLSVEDDTVSVAELLEDAQTPGPAEQLEYDEMLAALNRAADQLPPREKQLLTLYYQKE
                     MTMKEISNILHVSESRVCQLHMQAIARLRTTMDALAVTSVSRQPKQLQKERNRYQHAR
                     IAAKSR"
     gene            3883..>4051
                     /locus_tag="VGT44_08030"
     CDS             3883..>4051
                     /locus_tag="VGT44_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator"
                     /protein_id="HEV2580785.1"
                     /translation="MLVLRRKVGERIILDGVISISVLAVEGERVKIGITAPAEVSIVR
                     EELLRGVVDAPA"
BASE COUNT          798 a          994 c         1337 g          922 t
ORIGIN      
        1 cgcgagcatg agacgccgcg aaacgccacg tatttgcggc gtttctccac ctggatcgga
       61 tgggaaaatg cccggcgttg agacaggccc gttgggtatc gtctgagacc tgacctggac
      121 gggcgggaaa ggcatttgtg gaggcaagat gccgcgcggg gttgcttcca gtgttggatt
      181 agatgaaagc tgtgggtcgc tgacggcttc cggcaagggt gtcagaggtt ctgaagcctg
      241 ccgggtgatt gtgggccatt ctagctgcgg tgttgcctgc tgagttggag ggtatagcgt
      301 tttcgccgct tgcgggcgcg cgggaaaggc agcaggccgg gtgggcgtat aatcacttcg
      361 ctgcgtcggg ctgctggcct cttccagcgc agtaacgaag gctgtgaccg attcaaagcg
      421 ctgctggggg tcttttgcca gcgctctcat cattacctgc tcgatatcga gaggaacggt
      481 cgggagcttc tggctaagag aagggggtgg cacgaacata tgctggctgt atagttcgac
      541 gaaggtgccg tgaaatgggc gttccccact tagccattcg tagaggacga tgcccagcga
      601 gtattggtcg ctggcggggc gaggtttgcc ttgcagttgt tctggcgcca tgtaggcaac
      661 cgtgccgacg acctcttgtg tactttgcag gcgggagctt tgcgcgacga gggcgatgcc
      721 aaagtcgctc aagaggactt cgttattcgc gccaagcagc atgttctccg gcttgatgtc
      781 gcggtggatc agtttgcgcg tatgggcgta atgcaaagcg gcggcgacct gcctggcgta
      841 ggataacgct gtattgaatg ggaggcgcgt tccggtggga tgccgttggc ggagtgtgcc
      901 gttggcggca aagctcatga ccaggtaggg ggtagcgcct tcgatgccaa agtcaagcac
      961 gcgcacgatc gagggatgat cgagactggc aatcgtctgt gcctcttgta gaaaactgtc
     1021 caggtcggcg tcaactacct gggtgtgaag gactttaatg gctgcaggag ttttcaggaa
     1081 gatgtgcgtc gcgaggtaga cttccccaaa cccgccctta ccgagcgaac gaatgagacg
     1141 atagttacca agctgctgcc ctacgcgatc cgtcatatgg gatgcctctt acgattaatc
     1201 ttccacccaa tccacacccg gttcagtcga tctatacttt tgaacgtgct gtaaattgat
     1261 gagtaacagg atcacgcaaa ggcatagaag ttattagatg gtgaagatgg cctgtgtggg
     1321 gtgttgcagc aagccggcgg cgacgacttt gccggcgggc gcgcgagggg tgggtcccgg
     1381 gccggcttcc tggctgacgg cggcggtgtc gtagttgccg atagcgccag ggaagatgag
     1441 gctggcgatg ccattttgct cgctcagtac gccgatgctg gtgggcgcgc ctttgtgcaa
     1501 caaccagccc tggtagatgt ggctgccctg gagcgctggc aggttgtgca gcaccagcac
     1561 ggtgatgttt tgttggggca ggtagtagag cgagccgctg gcggtgtgcg cagcgggcgt
     1621 accggcgatg ttgaagacct gcgccacctg gcgattcagc gcgccgatct gacgcgcttg
     1681 cgcggcgttg ctttgttgaa gcgcgcttag ctggccggtt aattgcgtat tgctggcttg
     1741 cacggtggtc aactggtgtt gcagcgagac gttccaggcc gtcatgccgc cggtaatcag
     1801 gaagagcacg agggctgcga ctgccagtat gcgtgttgcc cacccggcgc ggcggcgtgt
     1861 gggttggcga acgcggcgcg gcggcggcgt tgcaacgggc ctgggtttct ggcgaatggc
     1921 ctggatgatg cgctctaaca acgcgggcga gggggtgact tgcggagcag cataaggcaa
     1981 gacgctcaca gccgcgcgta gctcctgggc caggcgggtg catttgtcgc aggtggcaag
     2041 atgctcctgc gctgcctggc gttcttccgg agtgacggca tccagcacat acgcgcctga
     2101 tagttcttca aattcttcgc aggtcatgca gactgctcct cggttagtct tcttgacctc
     2161 tattacgctg attgaggcaa gacagattac tataaccagg acagtatacc ttatggagcg
     2221 tctgcctgct acatggagcc gggttggagt gggaaaatgg gtgattgtga atgggcggag
     2281 gaggtgggat tcgaacccac gagggctatt caccctacac gatttccagt cgtgctcact
     2341 agaccactat gagactcctc caattggcgg ggcggagaga gtgggattcg aacccacgag
     2401 ggctttcacc ctaccgcttt tcgagagcgg cacattcgac cactctgaca tctctccgct
     2461 gcggagtata gcatatctgc cggggtgtgg caatatgttg aaaaaatgac cagggcgatg
     2521 gctggccctg gttgggggtt atgattcctg taattcgccc tgctcgtata accagcgcat
     2581 gaactgcatg tggtgatcgt tggcgatacg ctgctgcatc tcgccgttgg agtagagatg
     2641 atcgcgtaaa tagaacagtt tgacggcttc atcccagaca aacccgcgct gcatcaatgc
     2701 gtccatctgc gcttgctcgc tgttttctcc gatgggatcg ttcaggtcgg cctgccgcac
     2761 ggaatggtct ggccgctcta ctcgttcgct atgttcggat gattgtggag atggtgaatt
     2821 atttttcata tgagtacctc gcataacgcc tgcacaccag ggtaattctc tggtaatgct
     2881 ttttatcggc tttttgtctg ccgcccttga acgtcaagga gagggacttt cggcaatgca
     2941 aaccaacgac taatgccaat gtaaaaatac tgcttgcccg gctacactgg caacagtgat
     3001 gtggagagga ttgagtgtgg gaggaatgac tcaggaagga accttgtaag aaaggttgct
     3061 cgatgagtgc atttgcgacg aatgaggatg tctgggcaga gtttgtcacc acccgtcacc
     3121 cggcgtttcg cgatgagtta atcctcattt atatgccact ggtgcggttt gtggtgagca
     3181 ggctggggat tccgtcggtc ggtgtgctgg accaggaaga cctggtgagc ttcggggtga
     3241 tcggcctgat taacgccatt gatcgctttg agccggcacg gggcattcgc tttgaggcgt
     3301 tcgcgactcc gcgtattcgg ggcgctgtga tcgatcaact gcgggccatc aactggttcc
     3361 cgcgttcggc gatcaagcgc attcgacagg tggagggggc gttggcggag ttggaacagc
     3421 ggttggggcg ttctccgaca gaagtagagg ttgcgaccga aatggatgtc tcggtcgaac
     3481 gctaccgtca catgatgcag gaggccaaca cgacgatatt ttcgctcgat gcgccgttga
     3541 gttcgctctc ggtggaagac gatacagtgt cggtggccga actgctggag gacgcgcaga
     3601 cgcccggccc ggcagagcag cttgagtacg atgagatgct ggcggcgctg aaccgcgctg
     3661 ccgatcagtt gccgccgcgc gaaaaacagc tcttaaccct gtactatcag aaagaaatga
     3721 cgatgaaaga aatcagtaac atcttgcatg tttcggagtc gcgcgtctgc cagttgcata
     3781 tgcaggccat tgcgcgtttg cggacaacga tggatgcgct cgcagtcact tcggtctctc
     3841 gtcaaccgaa acaattacag aaggaaagga accggtatca acatgctcgt attgcggcga
     3901 aaagtcggtg aacgcatcat tctggatggc gttatcagca tatcggtgct ggcggttgaa
     3961 ggggagcggg tcaagattgg catcactgcc ccagctgagg tgtcgattgt gcgcgaggag
     4021 ttgctgaggg gggtggtgga tgcgcctgcg g
//
LOCUS       DASZAK010000460         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_508758, whole genome shotgun sequence.
ACCESSION   DASZAK010000460 DASZAK010000000
VERSION     DASZAK010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508758"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(187..453)
                     /locus_tag="VGT44_08035"
     CDS             complement(187..453)
                     /locus_tag="VGT44_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580786.1"
                     /translation="MDLERLEQRVLRCPLQELALRDETGCYVFGNDPVKPAWWYVGHQ
                     PIGGTWDFAQVRGLNNVVILVEQRYPALYTWLAHIAQGGEFACR"
     gene            complement(515..1090)
                     /locus_tag="VGT44_08040"
     CDS             complement(515..1090)
                     /locus_tag="VGT44_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580787.1"
                     /translation="MSSRVDRGHLFRVWDADRAHQRLAGLASWAAFSARWRAHQGLPL
                     LHPMDVQYLSPADFCNGRGQLLPPEVARRRFRDYVEAAIRCGVAMRAPLVGRRRAGLL
                     SADLGFARAAGFARWLGHHAPDYQYGRVDRPSYEPGALLPQRPYGVPVASNAFATQSA
                     YWLDDAQVGGDGDDDLGDDAWVAGFPDAPGW"
     gene            complement(1087..1293)
                     /locus_tag="VGT44_08045"
     CDS             complement(1087..1293)
                     /locus_tag="VGT44_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580788.1"
                     /translation="MEVVDQLIEAVNGALEAGDWAEVSRLTVNLYALAETDGDTQLLE
                     LVQDLHWIANDALVYPLDVQAVRA"
     gene            complement(1294..1620)
                     /locus_tag="VGT44_08050"
     CDS             complement(1294..1620)
                     /locus_tag="VGT44_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580789.1"
                     /translation="MEANGGGFLGAWPGGPGDGPFGAFGAYGKPLAEWCQRPVPSRLS
                     PGWFTCYCGCGVVGVCRHCVPHAPPWVPWRLCEQAERLVASGAFRCQGGWLEAVGESS
                     DDERRE"
     gene            complement(1624..1776)
                     /locus_tag="VGT44_08055"
     CDS             complement(1624..1776)
                     /locus_tag="VGT44_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580790.1"
                     /translation="MSKATLLGLCLFVFVCGLVWVFTWHLTGWFALLVIFLPLVLAVV
                     SVKEGV"
BASE COUNT          411 a          716 c          474 g          284 t
ORIGIN      
        1 caagaccagg caacgagacg ttcccgcccg tcgtggcgat gatctcggac ttgccactct
       61 cggacatgcg cagcgtttgc gacagatcaa ccgagatcag cagcaggttg ccattgacct
      121 ccatgcgaac attttccata catcacctca ctttcccagc gcacaaagtg gcgctgatag
      181 acgcactcat cgacaagcaa actccccgcc ttgcgcaatg tgagcaagcc aggtatacaa
      241 cgctggatac cgctgctcga cgaggatgac aacgttgttc agacctcgca cctgagcgaa
      301 gtcccacgtc ccaccaatcg gctgatgtcc aacataccac caggccggtt tcaccgggtc
      361 attcccaaac acatagcacc ctgtttcatc gcgcaacgcc agttcctgaa gggggcagcg
      421 cagcacacgc tgttctaatc gttccaggtc cattgctttc tcgttttctc tcgcaggcat
      481 gacgcgagca agaaccaacc cgctcaaccg acccctacca tccaggagcg tcagggaacc
      541 ccgcgaccca ggcatcatcc cccagatcat cgtcgccatc gcccccgacc tgcgcatcgt
      601 caagccaata ggcgctctgc gtcgcaaagg cattcgacgc caccggtacg ccatacggac
      661 gctgtggcag taacgccccc ggttcatacg acggcctatc aacacgccca tactgataat
      721 cgggcgcgtg atgtcccagc cagcgcgcaa acccggcagc gcgcgcaaag ccgagatcag
      781 cgctcagcag gccagcccgt cgtcgcccta cgagcggcgc gcgcatcgcg accccgcagc
      841 gaatcgccgc ctcgacatag tcgcggaagc gccgccgcgc cacttccggt gggagcagtt
      901 gcccgcgccc attgcagaag tcagccggcg agaggtactg cacatccatc gggtgcagga
      961 gcggcaagcc ctggtgcgcc cgccagcgtg cgctgaacgc cgcccagctt gccagccccg
     1021 ccaggcgttg atgcgcgcgg tcagcatccc acacccgaaa cagatgcccg cgatccaccc
     1081 gactgctcat gcgcgcaccg cctgcacgtc gagcggatac accagcgcat cattggcaat
     1141 ccagtgcaga tcctgcacca gttccagcag ctgcgtatca ccgtccgtct ccgccagcgc
     1201 gtacagatta accgtcagcc gggagacttc cgcccaatcg cccgcctcaa gcgcgccatt
     1261 gaccgcttcg atcagttgat ccacgacttc catctactcc ctcctctcat cgtcactgct
     1321 ctcccccacc gcttccagcc agccgccttg acagcggaac gcgccagagg ccaccaggcg
     1381 ctcggcctgc tcgcacaaac gccagggaac ccagggcggc gcatgcggca cgcaatgccg
     1441 gcacacgccc accaccccgc agccacagta gcaggtgaac cagcccggtg agaggcgcga
     1501 cggaaccgga cgctgacacc attcggctaa tggcttgcca tacgctccaa acgcgccgaa
     1561 aggaccatca ccagggccac ccggccacgc tcccaggaac ccacccccat ttgcctccat
     1621 gcgttacact ccctctttca ccgacaccac tgccagcacg agcggcaaga agatcaccag
     1681 cagggcgaac cacccggtca gatgccaggt gaagacccac accaggccgc atacaaagac
     1741 aaacagacac aatcccaaca acgttgcttt cgacatcgca tccctcctac gccgccgcgg
     1801 gcgtttcccg ccgggcaagc gccatttcca cccgcgccaa caggccaaag gcttgccgac
     1861 ccgtcatcct ctccgggacg ggcga
//
LOCUS       DASZAK010000461         1587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_11358, whole genome shotgun sequence.
ACCESSION   DASZAK010000461 DASZAK010000000
VERSION     DASZAK010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11358"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VGT44_08060"
     CDS             complement(<1..279)
                     /locus_tag="VGT44_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580791.1"
                     /translation="MSTLKVALLGPPEVEHLGRRLTFPDRKALALLAYFAAERGMHER
                     RKLSQLLWPESDAAHGRTALRIALYHLRHTLEVGTGPEHTPHLLIQHEM"
     gene            692..874
                     /locus_tag="VGT44_08065"
     CDS             692..874
                     /locus_tag="VGT44_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580792.1"
                     /translation="MSVTTTKALPRANGDFYAISSILGEEDSALLRKVRNFLEVDDGI
                     SACRWLPYIDVYTMSF"
     gene            889..>1587
                     /locus_tag="VGT44_08070"
     CDS             889..>1587
                     /locus_tag="VGT44_08070"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2580793.1"
                     /translation="MSASLIEHTPSAYEYPLLIKHLLHTPLATAPDQEIVYRDITRYT
                     YRTLRERIARLASGLASLGVKPGDTVAVMDWDSHRYLECYFAIPMMGAVLEMINIRLS
                     PEQILYTLNHAGADVILCFSDFLPILDAVRPNLVTVKTFVLLSDEGASAGKPGALVPF
                     AAEYEELLADSSPDFDFPDFDENTRATTFYTTGTTGNPKGVFYSHRQLVLHTMAALAT
                     IASTPEHQRFHRGDV"
BASE COUNT          342 a          479 c          433 g          333 t
ORIGIN      
        1 catctcatgc tgaatgagca ggtggggcgt gtgttcaggc ccggtgccca cctcgagcgt
       61 atggcgcaga tgatataaag caatgcgcag cgccgttctc ccgtgcgccg cgtcactttc
      121 aggccataag agttgcgaga gcttccggcg ttcgtgcatg cctctctcgg cggcgaagta
      181 agcgagcagg gccagggcct tgcggtcggg gaacgtcaag cgccgcccca aatgttccac
      241 ttcgggcggc ccaagcagcg caaccttcaa ggtactcatc gcttgctcct tcgcaacgct
      301 atcctgcccg agggcttcct tcaaaagaaa cgcaatgcgc gacaacaggg tactctcttc
      361 aacccgggta ttggagtggt tagagttagt atagtcaaca atagctgaga gagcaaggga
      421 ttccctttcg cgccacgaaa caaacgctcg ccttccacac ttaccgatca tataaacgct
      481 tttctcaacg ctcgccggat acactaattt caacagccga cgttctgacc aaacggcaag
      541 ggcttaagat gaaagcaaag cgggcaggcc atgctcgaag cccaggtgga gtggacgtgt
      601 cgctagccga cagggcatgc gcgctctgga gtatcatcgt cgattagctt actttacttc
      661 gtcagtgacc ttcaatgagg agggactacc tatgagcgtg actaccacga aggctcttcc
      721 gcgggccaat ggcgatttct acgccattag ttcgatattg ggcgaggaag attcggcact
      781 gctgcgcaag gtgcggaact tcctggaggt tgatgatgga attagcgcgt gtcgatggct
      841 cccgtacatt gatgtctata ccatgtcttt ctagaaagga gatttcccat gtcagcgtcg
      901 ctgatcgaac acacaccctc tgcatacgag tatccgctgc tcatcaagca cctgctccac
      961 acccctctgg cgacggctcc cgatcaggag atcgtgtacc gggatatcac tcgctatacc
     1021 tatcgcacgc tgcgcgagcg tatcgctcgg ctggcgagcg gccttgcctc actgggggtc
     1081 aagccaggcg atacggtggc ggtgatggac tgggacagcc accgctacct ggagtgttac
     1141 ttcgctatcc ccatgatggg agctgtcctg gaaatgatca acatccgcct gtcacctgag
     1201 cagatcctct acacgctcaa tcacgccggg gccgatgtca tcctgtgctt cagcgatttc
     1261 ttgcccatcc tcgacgccgt caggccgaac ctggtaacgg tgaagacctt tgtgctcttg
     1321 agcgatgaag gcgcctcagc cgggaagccg ggggccctcg tgccatttgc cgcagagtac
     1381 gaagaactgc tggccgacag ctcccccgat ttcgattttc ccgacttcga tgaaaacacg
     1441 cgcgccacga ccttctacac caccgggacc accggcaacc cgaagggcgt cttttatagc
     1501 caccggcaac tggtgctgca caccatggcc gcgcttgcaa caatagccag tacccctgaa
     1561 caccagcgct tccaccgggg cgatgtc
//
LOCUS       DASZAK010000462         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_294609, whole genome shotgun sequence.
ACCESSION   DASZAK010000462 DASZAK010000000
VERSION     DASZAK010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294609"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VGT44_08075"
     CDS             <1..202
                     /locus_tag="VGT44_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912506.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HEV2580794.1"
                     /translation="ATIHGALARTESRGAHYRLDYTSRDDANWLKHTLAYKGTKAHDV
                     RLDYKPVELIDDPIFKPKERKY"
     gene            214..921
                     /locus_tag="VGT44_08080"
     CDS             214..921
                     /locus_tag="VGT44_08080"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEV2580795.1"
                     /translation="MNVLMRIKRFNSEKDNRPYWDEFRVTMDPIDRLLDALNVVKWTM
                     DGTLTYRRSCAHGVCGSDAMRINGRNRLACKVLMRDVGERVTIEPMLGYPVIKDLVVD
                     MDSFFEKYKSVKPYLVTYDNEPGTERLQSPADRARYDEGTKCILCGACTGSCPSVWGN
                     PNWVGPATIVNAHRFIFDSRDQGAAERLAILGQRDGVWRCRTIFNCADACPRGIPVTD
                     LIEQVKRAIVYGDTRGE"
     gene            complement(1145..1354)
                     /locus_tag="VGT44_08085"
     CDS             complement(1145..1354)
                     /locus_tag="VGT44_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580796.1"
                     /translation="MTRQCAWCLRLMNQQGERISLQPVPKIYEATHGMCRVCGIHWLE
                     QALIDTGEQPALVPVLVAKEINFRC"
BASE COUNT          338 a          436 c          457 g          298 t
ORIGIN      
        1 ggccaccatc cacggggcgc tggcgcgcac cgagagtcgc ggcgcgcact atcgcctgga
       61 ttacaccagc cgcgacgatg ccaactggct caagcacacg ctggcgtaca aggggacaaa
      121 agcgcatgat gtgcgacttg actacaagcc ggtcgaattg atcgatgacc cgatcttcaa
      181 gccgaaggaa cggaagtact aggaggtaag acgatgaacg tattgatgcg tatcaaacgg
      241 ttcaattctg aaaaggataa taggccgtat tgggacgagt ttcgcgtcac gatggacccc
      301 atcgatcgcc tgcttgacgc gctgaacgtg gtcaaatgga cgatggatgg cacactgacc
      361 tatcgccgtt cgtgcgcgca tggtgtctgc ggctccgacg cgatgcggat caatgggcgc
      421 aaccgtctgg cctgcaaagt gctgatgcgc gacgtgggcg agcgcgttac gattgaaccg
      481 atgcttggtt atcccgtcat caaagacctg gtcgtcgata tggactcgtt cttcgagaag
      541 tataagtcgg tcaagcccta cctggtgacg tatgacaacg agccggggac ggagcgcctg
      601 caaagcccgg cagaccgcgc gcgctatgac gagggcacga aatgtatcct gtgcggggcc
      661 tgcaccggct cctgcccgtc ggtgtggggc aatcccaact gggtcgggcc ggccactatc
      721 gtcaacgcgc accgcttcat cttcgacagc cgcgaccagg gcgcggcgga acgcctagcg
      781 atcctgggcc agcgtgacgg cgtgtggcgc tgccgcacca ttttcaactg cgccgatgcc
      841 tgcccgcgcg gcatcccggt gaccgatctg atcgagcagg tcaaacgcgc cattgtgtat
      901 ggcgatacca gaggagaata acgttcagta cctccaaaag tagcaatccg gctctgcgca
      961 ctcggcgtct taaaagacga ccggaggggg tatgcgcgaa gccggatttg ccctacggac
     1021 ggaaggccgg gccgtttgct gcactctggg ggggagacga gcgcagcgca ctcgattcac
     1081 ttgcgtgaag agtacggccc accttccggc ggtcaaagac cgcctgctaa ggaaagggtt
     1141 tatcttaaca acgaaagttg atttcctttg ccacaagcac cgggacaagc gctggctgct
     1201 cgccagtatc gatgagcgcc tgctctaacc agtgtattcc gcacacgcgg cacatgccat
     1261 gcgtggcttc ataaattttt ggcaccggct gcagcgaaat tctctcgccc tgctgattca
     1321 tcagccgcag gcaccaggca cattgtcttg tcataaataa gtcgcgctcc cttccacacc
     1381 gataaaaata agaaccttgt tatacgcccc tacgggccgt tcgcaggcat cggattctga
     1441 ttggcacatc ctggaccggg cgcaggaagg ccgaagatgg aatttgagaa ctgcttgctg
     1501 gtgatatctt tcgcatagat cgagagtgg
//
LOCUS       DASZAK010000463         5955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_615833, whole genome shotgun sequence.
ACCESSION   DASZAK010000463 DASZAK010000000
VERSION     DASZAK010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5955
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615833"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VGT44_08090"
     CDS             <1..457
                     /locus_tag="VGT44_08090"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEV2580797.1"
                     /translation="YWYFKSKEDIIIAISQRLFSMDIEQLEGMLKSGGTVGERLLQLM
                     NSRAQGLLEMSAVITMLFEFYAAALHQDEVRRVIQEYFRGFHAVLVTLIQQGIDRGEF
                     RLINVEEAATTLASLFEGLVVRWLIDPQAVQWDTLIESSVRLFLDGLRM"
     gene            472..2859
                     /locus_tag="VGT44_08095"
     CDS             472..2859
                     /locus_tag="VGT44_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV2580798.1"
                     /translation="MQIQEREQELPPKTPARRSLDEAHGLYRVGIAYGRRIYRLRWVI
                     IAIWVVVVGASIPFTLKVGDVLTGGGYSYDNSESAKVDTIISTTVKQPPSQFLVVFQS
                     DTKLVGDAGYQQEVNAFISRARAYSHVTGIQQSGAGLDGRTMSVTVSLSISSSDATKN
                     INDFRAILPSGGAASPAHVYLSGGPAIDAAFNTITESDVEHAELAALPIALLVLLVVF
                     GTLVAATMPLLLAVVSVPVALAVIYAIAQHVTMSIFVLNIVSIIGLGISIDYSLFMLR
                     RFRDELASGGSIPDAVGNTIATSGEAILFSGLIVMIGFSALLLIGIPFMTSLGIGGAV
                     VVAIAALAALTLLPALLSVLGPRVNALRIPLIGRLTAPHAHKQQHENGNGDRVGVGSG
                     ANIDERRGFWQRWAEGVMRRPVLVILAVSAILIGLGWPIFSIELGTPSVSSLPTSDES
                     RRGIDILNAQFPETRIDPVYIVAQAPDGSSMLLGTNLTKLDTLSRWIARQAHVTGVVS
                     LTQLPATPGAPTLTEQELATLYSSGGYKQSPALAQLVASTTSGNTTIITVMTNAKLDS
                     SDGNALIDDLRSGDTTAGQGLTVLVGGSQASDLDFNRYLYGNFPRSVIFILIATYLLL
                     LLMFRSVFLPLKAILMNVLSVAACYGLLVFVFQWGNYSNILGFTPNGYLDSTNPILLF
                     CILFGLSMDYEVFLLSRIHEEWLRTHNNRYAVARGLEKTGGVITNAALLFVIVTGAFT
                     FTQLLITKEIGLGMSIAVLLDAAVIRTLLVPATMRLLGRWNWWLPGRPLPPKQAG"
     gene            3015..3308
                     /locus_tag="VGT44_08100"
     CDS             3015..3308
                     /locus_tag="VGT44_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580799.1"
                     /translation="MQPVTKSPFLQVSETQGSPITTPYGRITPVARAVKVRWRGGGFT
                     WNRAVAVEVDEGQQLRRVPIRDATSRTIGAIALTGLMIAACCGLFLRRRRRTS"
     gene            3305..3739
                     /locus_tag="VGT44_08105"
     CDS             3305..3739
                     /locus_tag="VGT44_08105"
                     /inference="COORDINATES: protein motif:HMM:NF021119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEV2580800.1"
                     /translation="MIDEKIAQSPVTNSIEQVFGQLANSARADVVFGQPVERDGATVI
                     PCAEVAVGLGMGTGSGPMDEHGRPMGGGGGGGGGSRGRPIAVIVMTRDGVRVEPVLDL
                     TKIVLGSLTTGAFMLIWLGRLARMNRGGKGPSLSQLKRAIES"
     gene            3831..4688
                     /locus_tag="VGT44_08110"
     CDS             3831..4688
                     /locus_tag="VGT44_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580801.1"
                     /translation="MSISTISWRARLLTVALTLVIVTSVVVGVAVVRNHHATSDAAAL
                     HPASVSKAMVTISRGQDRFEPFILPVTPGTSVTWKNNDNVTRTFGTTPDRRSFLNPQP
                     FSFKVGAGQHATYQFTMPGIYHYYETTKGTWNATFSRVTADNGLRHYPLSMEGIIWVQ
                     GAIVGLPLSVYNYVLAGHDMFAHEFIAIAVDGAVTWRNTDTDPHFVGPVAGWSAPVNP
                     VDMGLYRLGGTNEVPGGESVSVIFTTPGLYYYYCRNHDLVDPPTDRVQARTMASEYPI
                     PMEGFVLVV"
     gene            4937..>5955
                     /locus_tag="VGT44_08115"
     CDS             4937..>5955
                     /locus_tag="VGT44_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEV2580802.1"
                     /translation="MPIAVRLGLGFLVPALLAAIALGGVGIRSQQLLSHESKYFQILV
                     DSYTTLKSESDMIQQIHANLLGLQSDAGERQPSPTTITEDLAIIDLLTSRFDVALDSY
                     LRADTLDHSPDLVALLADAGHSDQVRQQAQDTAQFNNSWDAYAAQQREVSESLKAGKV
                     YQASFAEVALVEPAYANVMSSLLRLLQFTNTLAPSIHDALNIEESRLLFDATLAAFSI
                     LIGIGIVGWLVFYTVARRLQKARRVMKVIEGGQIETRLPVVGRDEITSVCSGVNGMLD
                     TIVGLLEETRQQRDELASAEELKQLHQELQSKHEALNEANARLEALATTDPLTGLPNH
                     RTVMGR"
BASE COUNT         1201 a         1591 c         1887 g         1276 t
ORIGIN      
        1 gtactggtat ttcaagagta aagaggacat tattatcgcc atttcgcagc ggctgttcag
       61 catggatatc gaacagttgg aggggatgtt gaagtcgggt gggacagtcg gcgagcgcct
      121 gctgcaattg atgaacagcc gggcgcaggg gctgctagaa atgtctgcgg tgataacgat
      181 gctgtttgag ttttatgcag ccgctcttca ccaggatgag gtgcggcgcg tcatccagga
      241 atattttcgg ggtttccatg cagtgctggt cacattgatt cagcagggca tcgaccgggg
      301 ggagttccgg cttatcaacg ttgaagaagc ggccaccacg ctcgcatcct tgttcgaagg
      361 tctggttgtg cgctggttaa tcgatcccca ggcggtgcag tgggacacgc tgatcgaaag
      421 ttcggtacga ttgttcctgg acggtttaag gatgtaagtg agagcaaaca aatgcagata
      481 caggagaggg agcaagaact gcccccgaag acgcccgcgc gccgttcgct ggacgaggcg
      541 catggactct accgcgtggg gattgcctac ggacgccgca tctatcgcct gcgctgggtt
      601 atcatcgcca tctgggttgt cgtggtgggc gcgagcattc ctttcacgtt gaaagttggc
      661 gatgtgctga ctggcggcgg ctacagttat gacaacagcg aatcggcaaa ggttgatacg
      721 attatttcca cgacagtgaa gcagccgccg tcgcagttcc tggtggtctt ccaatccgat
      781 accaaactgg ttggtgacgc cggttaccag caggaggtga atgccttcat cagccgcgcg
      841 cgcgcctatt cgcatgtgac cggcattcag caaagcggtg cggggctgga cggccgcacg
      901 atgtccgtga cggtctcgct gagtatatca agtagtgacg ctacgaaaaa catcaatgat
      961 ttccgcgcca tcctgccgtc gggcggcgcg gccagccccg cgcatgtcta tctgagcggc
     1021 gggcctgcca tcgacgcggc cttcaatacg attaccgagt cggatgttga acatgccgag
     1081 cttgcggctc tacctatcgc gctgcttgta ctgctagtcg tcttcggcac actggtggct
     1141 gctacgatgc cgctgctgct ggctgtggta tctgtgccgg tagcgctggc cgtcatctac
     1201 gccattgcgc agcatgtcac catgagcatc ttcgtactga atatcgtttc gatcattggc
     1261 ctgggcatct cgatagatta cagccttttc atgctgcggc gtttccgcga tgaactggcg
     1321 agcggcggca gtatccccga cgccgtcggc aataccatcg ctacgtccgg cgaggcgatt
     1381 ctgttcagcg gcctgatcgt tatgatcggc ttttctgcct tgctgctgat cggtattccc
     1441 ttcatgacct cgctcggcat cggcggcgcg gttgtggttg ccatcgccgc gctggcggcg
     1501 ttgacgctgc tgcccgcact gctgagcgtg cttggcccgc gcgtcaacgc gctgcgtatc
     1561 cccctgattg gccgcctgac cgctccccat gcgcataagc agcagcatga gaatggcaat
     1621 ggagaccggg ttggcgtggg aagcggagcg aacatcgatg aacgaagagg attctggcag
     1681 cgctgggccg agggggtgat gcgccgtccc gtgctggtga ttctggcggt ttcggccatc
     1741 ctgattggcc tgggctggcc gatcttctcg attgagcttg gcacaccatc cgtttcatcg
     1801 ctgcccacca gcgatgagtc gcggcgcggc atcgacatcc tgaacgcgca attcccggaa
     1861 acgcgcattg acccggtcta catcgtagcg caagcgcccg atggttccag tatgcttttg
     1921 ggtacgaacc tgacgaagct ggataccctg tcgaggtgga ttgcgcggca ggcgcacgtg
     1981 accggcgtcg tcagcctgac gcagctgccg gcgacaccgg gcgcgccgac tctgacagag
     2041 caggagctgg cgacgctcta tagcagcggc ggctacaagc agagtccggc cctggcgcag
     2101 ctggtcgcct cgacgacgag cggcaatacc acgattatca ccgtgatgac gaacgcgaag
     2161 ctggacagca gcgacggcaa tgcgctgatc gatgacctgc gtagtggcga tacaacggcg
     2221 ggacaggggt tgacggtgct ggtgggcggg tcgcaggcgt ccgacctgga cttcaaccgc
     2281 tacctctacg gcaatttccc acgcagcgtc atcttcatcc tgatcgctac ctacttgctg
     2341 ctcctgctga tgttccgctc ggtcttcctg cccctcaagg cgatactgat gaatgtgttg
     2401 tcggtggcag cctgttatgg actgctggtc tttgtcttcc agtggggcaa ctatagcaac
     2461 atcctgggct ttacgccgaa tggctacctg gatagcacca acccgatcct gctgttctgc
     2521 atcctgttcg gcctctcgat ggattacgag gtcttcctgt tgagccgcat tcacgaggag
     2581 tggctgcgca cgcacaataa tcgttatgcc gtggcgcgcg gcctggagaa gacagggggt
     2641 gtgattacca atgcggcgct gctcttcgtg atcgtgacgg gcgcgttcac cttcacgcaa
     2701 ctgctgataa cgaaggagat cggactgggg atgagcatcg ccgtgctgct cgacgcggcg
     2761 gtgatccgca cattgcttgt gcctgccacg atgcgcctgc tcggcaggtg gaactggtgg
     2821 ctgcccggca gaccgctgcc gccgaagcag gcaggatgag cggtcgccat gcctgttatc
     2881 gggaaaatgt gatgcatttg gtaagttcag cgtacaacat tggcatgacg tggaacagga
     2941 atgtggtaag ataacgatgg cgagattgac agcctcatag tgcgtttcaa aggagtgtag
     3001 ggagcggaga acgaatgcag ccagttacaa aatcgccttt tttgcaggtg agtgagacgc
     3061 aaggaagccc gataactact ccttacggtc ggattacccc ggttgcgaga gcagtgaagg
     3121 tgcgctggcg cggtggtgga ttcacctgga atcgagcagt ggccgtcgag gttgacgagg
     3181 ggcagcaatt gcgcagggtg cctattcgag acgctacttc gcgaacgatc ggcgctatcg
     3241 ccctgaccgg gttgatgatc gccgcatgtt gtggcttgtt tctcagaaga aggagaagga
     3301 catcatgata gacgagaaaa tcgcacaatc ccctgtgacg aactcaattg agcaggtttt
     3361 tggacaactc gctaactctg cccgtgccga tgtggtcttt gggcagcctg ttgagcgcga
     3421 tggcgcgacg gtcattcctt gcgcggaggt cgcggtcggt ctgggcatgg gaaccggcag
     3481 tggaccgatg gacgagcatg gaagaccgat gggcggtggc ggcggcggcg gcggtggcag
     3541 ccggggacgc ccaatcgcgg tgatcgtaat gacgagagac ggggtgcgcg tcgaaccggt
     3601 tctcgacctg acgaagatcg tgcttggttc gctcacgacg ggagctttca tgctcatctg
     3661 gctgggcagg cttgcgagaa tgaatcgcgg cggcaaagga ccttccctat cacagttaaa
     3721 acgagctatt gagagttagg acgcacccat agcggaacaa acgcctttgg ctctcctcgc
     3781 tatactgacg gtaatatcag ttacttgtca ggaagtgaga ccatcgagtc atgtccatat
     3841 ctactatatc ctggcgcgcg cggttgctga ctgttgcgct gacgctggtg attgtgacga
     3901 gcgtcgttgt tggcgtggca gttgtacgca accaccacgc cacctcagat gccgccgcac
     3961 tgcatcccgc ctcggtgagt aaggcgatgg tgacgatctc gcgcgggcag gatagatttg
     4021 aaccgttcat tctgccggta acgccaggaa ccagcgttac ctggaagaat aacgataacg
     4081 ttacccgcac cttcggcacc acgccagatc gacggagttt tctcaatcct caaccgttct
     4141 ccttcaaggt gggagctggc cagcatgcga cctatcagtt taccatgccg ggcatctatc
     4201 actactatga gacgacgaag gggacatgga atgccacatt ttcgcgcgtg acggcggata
     4261 atggcctgcg tcattacccg ttgtcgatgg aaggcatcat ctgggtgcag ggggcgattg
     4321 ttggtctgcc cctgtcggtc tataactatg tgttggcggg acacgatatg ttcgcgcacg
     4381 agtttatcgc tatcgcggtc gatggggcag taacatggcg taacaccgat accgacccgc
     4441 atttcgttgg ccccgttgcg ggctggtcgg caccggtcaa ccccgtcgat atgggcctct
     4501 accgcctggg tggaacaaac gaggttcccg gtggagaaag cgtcagcgtc attttcacca
     4561 cgcccggctt gtactactac tactgccgca atcacgacct ggtggacccg ccgacagacc
     4621 gggtgcaggc gcgcacgatg gcctcggaat acccgattcc gatggagggg tttgtgctgg
     4681 tagtgtgagg ggcgtgtagc gctcaagggg tgtagaacag ctcacgaatg ccgatacatt
     4741 tctgcataga gcagtaaagc tgctcagcta tgccaggaat gtgaggcaca attacgtgag
     4801 cgtatctcta caagagtcac ctgcgtttca aggtgaacgg gtacctgagc ggcaggagcg
     4861 tcaagatgat ggaaggggtg ataggagcgg cggcaaaaag cgcaatgggg gacgtagaag
     4921 aacgcctctg ttgaacatgc cgatcgccgt gcgactgggc ctggggtttc tcgtaccggc
     4981 gctgctggct gctatagctt tggggggtgt agggatacgc agtcagcagc tgctctcaca
     5041 tgagtcgaag tattttcaga ttctggtcga tagctataca acgctcaaga gcgagagcga
     5101 tatgatccag cagattcatg cgaatctgct tgggctacag agcgatgctg gcgaacggca
     5161 gccatcgccg acgacgatca ccgaggacct tgccatcatc gatttgctca cctcgcgttt
     5221 tgatgtggca ttagatagct atcttcgggc ggatacgctc gatcactcac cggacctggt
     5281 ggccctgtta gccgatgcgg gacacagcga ccaggtacga cagcaggctc aggacacagc
     5341 gcagttcaac aattcctggg atgcttacgc cgctcaacag cgagaggtaa gcgagtcgct
     5401 gaaagcgggc aaggtgtacc aggcatcatt cgcggaggtc gcgctggtag aaccggccta
     5461 cgccaatgtg atgagttcgc tgctgcggct gctccaattc actaatacgc tggcgccctc
     5521 cattcatgat gccctgaata tcgaggaaag ccgacttctg tttgatgcaa cgctggctgc
     5581 cttctcgatc ttgatcggta tcggcattgt gggctggctg gtcttctaca cggtggcgcg
     5641 acgcttgcaa aaagcgcgac gggtgatgaa ggtgatcgag ggtgggcaga tcgagacgcg
     5701 cctgccggtt gtggggcgcg acgagatcac gagcgtgtgc agcggcgtga acgggatgct
     5761 ggatacgatt gtgggattgt tggaggagac caggcagcag cgcgacgaac tggccagcgc
     5821 cgaagagctt aaacagttgc accaggagtt gcagagcaaa cacgaggcgc tcaacgaggc
     5881 caatgcgcga ctggaggcgc tggcgacgac cgatccactg acggggctgc ccaatcatcg
     5941 cacggtgatg ggccg
//
LOCUS       DASZAK010000464         1678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_11515, whole genome shotgun sequence.
ACCESSION   DASZAK010000464 DASZAK010000000
VERSION     DASZAK010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11515"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VGT44_08120"
     CDS             <1..590
                     /locus_tag="VGT44_08120"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV2580803.1"
                     /translation="HRQAWKRLAQEEGVRFSDADFWSTFGRRNDDIITLVWGAQSPER
                     LKELANRKEAYFRDLIRKTAAPLPGSIELLRGLHEAGFLQALASSAPIENIQLISDVL
                     GLGQYLTALVSGETVAHGKPAPDIFLKAAEELGVDALECLVIEDAVAGVQAAQAAGMR
                     CIAVAGDRDLPGLREAELMVKSLTEVDVERVRSLA"
     gene            630..725
                     /locus_tag="VGT44_08125"
     tRNA            630..725
                     /locus_tag="VGT44_08125"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:664..666,aa:Sec,seq:tca)
     gene            944..1600
                     /locus_tag="VGT44_08130"
     CDS             944..1600
                     /locus_tag="VGT44_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2580804.1"
                     /translation="MTKGAGKNQERIARRQVDKFAERRAQLAASALQTLSELGYARTS
                     LRDIAQHSEFSHGVLHYYFSDKVDLIIQSVKQYKTECVKRYDEIVATARTAMELKHGF
                     SAALAGTLRDDALMHRLWYDLRNQSLFEESFRADVLEIDQSLQRMIWRIVSQYAELAD
                     APLAVSSSTAYAIFDGLFQQALLRQLAGHADAGRQLQESVESIIDHLIVEAEVGERIS
                     "
BASE COUNT          354 a          469 c          517 g          338 t
ORIGIN      
        1 aacatcgcca ggcatggaaa cgtttagcgc aggaagaggg cgttcgcttc agcgatgccg
       61 acttctggtc aactttcggc aggcgcaacg atgacattat cacccttgtg tggggtgcgc
      121 agtctcctga gcgtttgaag gaactggcaa accgtaaaga agcttacttt cgcgatctga
      181 tccgcaagac tgcggctccc ttgcctggtt ctatcgagtt gctgcgtggt ttgcacgagg
      241 caggtttcct gcaagcattg gcatcctcgg ctccaatcga gaatatccag ctcattagcg
      301 atgtgttagg actgggacaa tacctcacgg cgcttgtctc tggcgaaacg gtggcacatg
      361 ggaaacctgc cccggatatc ttcctcaaag ctgctgagga acttggcgta gacgctttgg
      421 aatgcctggt tatcgaggat gcagtggcgg gagtgcaggc ggcgcaggcc gctggtatgc
      481 gctgtattgc ggtggcaggc gaccgtgatc tgcctggttt acgcgaggcg gaattgatgg
      541 tgaaatccct gacggaggtt gatgtggagc gggtgcgctc gctggcttga caagagtgtg
      601 cggtgatggc acaatacagc cacacacatg gaggtgtagg ggccccggtg ggtctcaccg
      661 tcttcaaaac ggctgggggg tcgctcggcg cggtccctgg tgggttcgac tcccatacgc
      721 ctctgccggt tagttgaaac atgtaattgt agctacaccc agaaagcgga cgaaaacgtg
      781 gctctgcaga agaaagcctc gccatcgctg gcagcccaat tcctcacaaa cgaccgggag
      841 aagaagcatc tgacgcaagt gcacctcgca gaagacctga agggagagcc acgcaccctg
      901 cgcgcctgga aggacggcgt gaggagccga cggaggtcgc aacgtgacga aaggtgccgg
      961 taagaaccag gagaggatcg cgcgtcggca ggttgataag ttcgccgagc ggcgggcgca
     1021 gttggccgcg tcggctctac agacgctctc cgagctcggg tacgcccgta cgagcctgcg
     1081 cgacatcgcg cagcactccg agttctcgca cggtgtcctg cactactact ttagcgacaa
     1141 ggtcgacctg atcatccagt ccgtcaagca gtacaagaca gagtgcgtga agcgctacga
     1201 cgagatcgtc gcgaccgcac gtacagccat ggaactcaag catggcttca gcgctgccct
     1261 ggccggcacc ctgcgcgacg acgctcttat gcaccggttg tggtacgacc tgcggaacca
     1321 aagcctgttc gaagagtcat tccgcgctga tgtgctcgag attgatcaga gcctccagcg
     1381 gatgatctgg cgcatagtca gccagtatgc tgaactcgcc gatgccccac ttgccgtgtc
     1441 ttctagtaca gcatacgcga tcttcgatgg gctattccag caggccttgc tcaggcaact
     1501 cgccggacac gctgatgctg ggcgccagct gcaggagagc gtcgaatcga taatcgacca
     1561 cctcatagtc gaggctgagg tgggtgagcg gatctcttag tagcgcccct gaccctgcca
     1621 gtgcacgtct tgcttaattt cttaccacgc ctccgtctta cctcgcagca gggaagaa
//
LOCUS       DASZAK010000465         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_153608, whole genome shotgun sequence.
ACCESSION   DASZAK010000465 DASZAK010000000
VERSION     DASZAK010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153608"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VGT44_08135"
     CDS             complement(<1..463)
                     /locus_tag="VGT44_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2580805.1"
                     /translation="MPARIIIADDETIQRMDLKDILAKQGYLVVGEAGDGVSAVNLAR
                     ELRPDLVIMDIRMPDMDGITAAEALTQEKIAPVLLLTAFGDQQLVERAKDAGVVNYIV
                     KPLRESEVAPAIEVTLARYNEFRLLEEKTRTLTEQLETRKIVERAKGVLMEK"
     gene            complement(603..1451)
                     /locus_tag="VGT44_08140"
     CDS             complement(603..1451)
                     /locus_tag="VGT44_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580806.1"
                     /translation="MNQETNSEAQDNRQERSTSSKRGSRAERNRPVLVTPDADSEVTQ
                     DQPAKEISDATQPVAEVATTTTDEAPRPRRLPKFFSSVARNEQQQAITANPEAARIAR
                     ATRSANSRPAKESKAEESSAPAKTSADQTPAKAAPATSSRPRPASAFKMRYLFGILLY
                     LILAELIGGFERSLLIANKLDKLLFQIGPLAVTTSTLAFLLTLIVILVILARLDLVPR
                     SLAALSGQPTPQRRPGQSQNTSNSTGIKTPPPAMKQGVKGEDDDLYQEYREQQRYLQR
                     RERRKR"
     gene            complement(1448..>1787)
                     /locus_tag="VGT44_08145"
     CDS             complement(1448..>1787)
                     /locus_tag="VGT44_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580807.1"
                     /translation="GGATVGGIGVSGGNAPGRDDEIARAGLEAGETMPAMQPSNVTPQ
                     PSLRSPVQPPYSLVPPAQQQPHFASSVNSPAPQSTQANTYHLGSDQDNPYGDEQLPFD
                     QFNETRGDQS"
BASE COUNT          336 a          473 c          509 g          469 t
ORIGIN      
        1 gtttctccat caatacaccc ttggcacgtt cgacaatctt gcgtgtctcg agttgttctg
       61 tgagggtacg agttttttct tcgaggaggc gaaactcgtt gtaacgagcc agggtaacct
      121 caatggcggg cgcaacctcg ctttcgcgta gtggtttgac gatgtaatta acaacgcccg
      181 catccttggc gcgttcaacc agttgctggt ctccaaaggc cgtgagcagc aagaccgggg
      241 ctattttttc ctgtgtgagc gcctcggcgg cagttatgcc gtccatgtca ggcatgcgaa
      301 tatccatgat taccaggtct ggacgtaact cacgggccag gttaacggcg ctcaccccat
      361 cacccgcctc gccaaccacc aggtaaccct gtttagccag tatgtctttc agatccatgc
      421 gctggattgt ttcgtcgtct gcgataatga tacgtgcagg catcgtgttc tctctctcgt
      481 tgcatacaat accacgcccc acatccaccc ggctttattt aaaggcgaac tggcttctat
      541 agccaggata agtacttcag cagtaccggt accgaaagta taccataaaa ctgtcgttcg
      601 cactatctct ttcgtcgttc gcggcgctgg agatagcgtt gttgttcgcg atactcctgg
      661 tacaggtcgt catcctctcc ttttacgccc tgtttcatcg ctggcggcgg ggtcttgata
      721 ccggtggaat tagaggtatt ctgagactgg cccggcctgc gttgaggtgt tggctggcct
      781 gacagcgctg caagactacg aggaacgagg tcgaggcgcg ccagtatgac cagtatgacg
      841 atcagggtca acaagaaagc gagagtcgat gtagtgacgg ctaaaggccc tatttggaag
      901 agcagcttat ccagtttgtt ggcaattaac agacttcgct caaaccctcc gattaactcg
      961 gcaaggataa gatagagcag tatgccaaag aggtagcgca tcttaaacgc gctcgccggc
     1021 cggggccggc tagaagttgc tggggcggcc tttgcaggcg tctgatctgc gctcgttttg
     1081 gccggtgcac tggattcctc cgctttgctc tctttggccg ggcgactatt cgcgctgcgc
     1141 gtcgctcgcg caatgcgtgc agcctccgga ttggctgtga tagcctgctg ttgttcgttt
     1201 ctggcaacgc tagagaagaa tttgggcagc ctgcgcgggc gaggagcctc gtctgtcgtc
     1261 gttgtggcaa cttcagcgac tggctgcgtg gcgtcgctta tttccttcgc tggctgatcc
     1321 tgtgtcactt cgctgtctgc atcaggagta acgagaacgg ggcgattgcg ctctgctcgg
     1381 ctgccgcgtt tagatgaagt ggacctctcc tggcggttgt cctgcgcttc tgagttagtt
     1441 tcttgattca tgattgatct ccacgggttt cattgaattg gtcaaaaggc aactgctcat
     1501 caccgtatgg attatcttga tcagaaccaa ggtgatatgt atttgcctgg gtgctttgcg
     1561 gcgctggcga gttaaccgaa ctagcgaagt gcggctgctg ttgcgccggt ggcaccagtg
     1621 agtatggtgg ttgtacaggt gatcgtagtg atggttgtgg tgtaacgttt gacggctgca
     1681 tagcgggcat tgtttcccct gcttccaggc cagcgcgtgc aatttcatcg tcccgtccag
     1741 gggcatttcc gccgctcacg ccgataccgc ccacggtagc accgcct
//
LOCUS       DASZAK010000466         2940 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_153619, whole genome shotgun sequence.
ACCESSION   DASZAK010000466 DASZAK010000000
VERSION     DASZAK010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153619"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            554..2908
                     /locus_tag="VGT44_08150"
     CDS             554..2908
                     /locus_tag="VGT44_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEV2580808.1"
                     /translation="MQRHLVRNVLFLVISILLITASLLAIRANARADQNAPTPLTAQV
                     VPLIQHAQMLQATDASQQLNLSVGLQLNNQAQLVSMLSAMYDPQSPQYQQYLTPDQYE
                     SLFAPTADQVQQVVTYLQSQGLSVTSVASNRMLIDATGSVAQAEQAFSTQINNYQLGA
                     QTFYANATPPVVPGSISQLITSIGGLDNSTQYQPLLTRTAQLAKTSIQGPSAGFGPKE
                     LSKAYNATPLQNAGILGDNQTIALFELDGYQSADVNQYFTNYGIATPNISNVLVDNYN
                     GAAGQGAIEVELDIEVIGGMAPHANQLVYEGPNTTQGLNDTYNQIVHDHKAQVVSISW
                     GLCETSTGAAELQTLDNIFKQGAAEGISFFAASGDSGAYDCNDSNLAVDSPADDPYVT
                     GVGGTTLQLSGGVYGSESVWSDASATQRSPKGAGSGGGLSSVFTLPAWQSGQGVMNPN
                     SSGAPCNAPSGQFCREVPDVSANADPNTGYAIYCTVNNAGCPSTGWMDVGGTSAAAPL
                     WAGSTALINQFLLSKGKAIIGSANPALYALLNAQPALSPFHDVTSGNNLYYPAGTGYD
                     MASGIGSPNVNNMAQDQVLPGNGGGSPTPTPTTTPVPSPSPTVTVTVSPSPTNTPAPP
                     PPTLIQNGGFEKGQSPWQEYSSGGYEIIQNLNAHSGQYSAYMCGYPGCDDRISQTFIV
                     PTSYTMLTVSYWWYSDTNKTVNKCLDTFNSRMQTPKGALIGKMQQSCNTNVTNNWVLE
                     TYDVSAMLANYKGSKVTLFFEGTNVANQYQPTDFFVDDVAVTAS"
BASE COUNT          679 a          796 c          873 g          592 t
ORIGIN      
        1 ggtgggcatt gtgctcgctc tgatcttcct gcctgggaga gccgcctcga aagctggagg
       61 ggccgaggcg gtgggggagt cacagcatgt cagtgagaaa ctgacggaaa tcgtagggta
      121 aaggatacaa gtcagcattc aatgggacga aattggtaca atgatgcgag taactgctcc
      181 tcaacaaaaa gcagacgact cggaaaagta tcaaacttgc acccattgaa tgctcacata
      241 tatagcttga cccaggtgtg aagagatata ttgctccatt cacctcgtcc agcgcgggat
      301 gagacgcatt tttcacatgc gagcagggcg gtgatattgc aggttgaatc cgcaaactca
      361 acggcgaacc tcgtttattc ttgcaagact tttagcgctc ctttatggag gggcaaaaag
      421 atatattacg ggtatcaagg gcaatgagat agggaagtct tgcctggaga gagctgtttt
      481 gtagcgtgtt atcgtattga cgatccctgg gggtcgtgcg ggtggcccaa aagtcaccca
      541 ggcggagtat tgtatgcaac gtcatcttgt acgaaacgtc ctgtttttag ttatttcgat
      601 actgttgatt acggccagcc tgctggcgat acgcgcgaac gcgcgcgccg atcagaacgc
      661 gcctacccca ctgaccgcgc aggttgtgcc acttatccag cacgcgcaga tgttgcaagc
      721 caccgatgca agccagcaat tgaacctgtc ggtgggtctg caattgaaca accaggcgca
      781 acttgttagt atgctcagcg cgatgtacga tccgcaatca ccgcagtatc agcagtattt
      841 gacgcccgac cagtatgagt cgctcttcgc gccaaccgcg gaccaggttc agcaggttgt
      901 gacgtatctc cagagccagg gtctgagcgt gacgagcgtc gcgtctaatc gtatgttgat
      961 cgatgcgacg ggcagcgttg cgcaggccga gcaggcattc agcacacaaa tcaataatta
     1021 tcaattgggc gcacagacat tttatgctaa tgcgacaccg ccggttgttc ccggctctat
     1081 cagccagtta atcacttcta tcggcggcct tgataacagt acgcaatacc agccgctttt
     1141 aacgcgcact gcgcaactcg cgaaaacatc gatacaggga ccgagcgccg gttttgggcc
     1201 gaaagaattg tcaaaagcat acaatgcgac gccgctccag aacgccggaa tactgggtga
     1261 taaccagacc atcgcgctgt ttgaactgga tggctaccag tctgcggatg tgaaccaata
     1321 tttcacgaat tatggtatcg cgacgcccaa catcagcaat gtgctggtcg ataactacaa
     1381 tggcgcggct ggccaggggg caattgaggt agaactggat atcgaggtga ttggcggtat
     1441 ggctccccac gcgaaccagc ttgtgtatga ggggccgaat acgacgcagg gcttgaacga
     1501 tacgtataac cagatcgtgc atgaccacaa ggcgcaggtc gtcagcatta gctgggggct
     1561 gtgcgagacc agcacgggcg cggcggagtt gcagacactc gacaacatct tcaagcaggg
     1621 cgcggcggag ggtataagct tcttcgcggc ctccggcgac tctggcgcgt atgactgcaa
     1681 cgatagcaac ctggcggtcg attcgcccgc cgatgacccg tatgtgacgg gcgtgggcgg
     1741 gacgacgctg caattgagcg gcggggtgta tggcagcgag tcggtgtggt ctgatgcgtc
     1801 agcgacgcag cgctcgccga agggtgcggg cagtggcggc ggcctcagca gcgtcttcac
     1861 gcttccggcg tggcagagcg ggcaaggtgt gatgaacccc aacagcagcg gcgctccctg
     1921 caacgcgccc agcgggcagt tttgccgcga ggtgccggat gtctcggcga atgccgatcc
     1981 caataccggt tatgccatct actgcacggt caacaatgct ggctgcccct cgacgggctg
     2041 gatggacgtg ggcgggacga gcgccgccgc gccgctgtgg gcgggcagca cggcgctgat
     2101 taatcaattc ctgctatcga agggcaaggc cataatcggc agcgccaatc ccgcgctgta
     2161 cgcgcttttg aacgcgcagc ccgcgctctc gcccttccac gacgtcacaa gcggcaacaa
     2221 cctgtactat cctgccggta ccggctacga catggccagc ggcatcggct cgccgaatgt
     2281 gaacaatatg gcgcaagacc aggtcttgcc gggcaacggt ggcggctcac cgacgccgac
     2341 gccgacgacc acgcccgttc cgtcgccatc cccaacggtg acagtcacgg tttccccttc
     2401 gccgacgaat acgcccgctc cgccgccgcc cacgctaatc cagaacggcg gattcgagaa
     2461 ggggcagtca ccctggcagg aatactcctc gggtggctac gaaatcatcc agaacctgaa
     2521 cgcgcatagc gggcaatata gcgcgtacat gtgcggctat cctggctgtg atgatcgcat
     2581 ctcacaaacg ttcatcgtgc caaccagcta tacgatgctg acggtgagct actggtggta
     2641 ttccgacacg aacaagacgg tgaataagtg cctggatacc ttcaatagcc gtatgcaaac
     2701 ccccaagggc gcgttgatcg gtaagatgca gcagagttgt aataccaatg tgactaacaa
     2761 ctgggtgctg gaaacctacg atgtctcggc catgctcgcg aactacaagg gtagtaaggt
     2821 gacgttgttc ttcgagggga cgaacgtggc gaaccagtac cagccaacgg atttctttgt
     2881 ggatgatgtg gcggtgacgg cgtcgtaggg atgcgataat gattttttaa cgagcaagtc
//
LOCUS       DASZAK010000467         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_117525, whole genome shotgun sequence.
ACCESSION   DASZAK010000467 DASZAK010000000
VERSION     DASZAK010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117525"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..736)
                     /locus_tag="VGT44_08155"
     CDS             complement(125..736)
                     /locus_tag="VGT44_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2580809.1"
                     /translation="MARAFSEEEKAHLQQRLVELGRTLFLSQGLKKTSLEDLTQPLGI
                     AKSTFYLFFESKEDLYLELLMQERARLEQQVMAASFDTTADVREAIIRFMHVVIDEME
                     RSELTRRLMTHPEELALLIRQGSSQQLAAHLQDSLGKIRPFIQRGQETGYVIDGNPET
                     IVGVLLAVIRLLTFYKEPIGQDAYPGVRDLLINLVASGLTHNA"
     gene            851..2293
                     /locus_tag="VGT44_08160"
     CDS             851..2293
                     /locus_tag="VGT44_08160"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2580810.1"
                     /translation="MIESREDPESTEEEREMEWNPTWEDPLTSNVKQFDGLMGDQRTR
                     KTFGEVVKGILAAGSLICQQIAARSAELSRGKKGSQRVIRLATGESTQRSELDAEHLT
                     QRLREVAVEQLAQAPEDELWLIGDSSDLRKPYAEVMPYLMPVRDLDDQLVPGYRTLNV
                     LGLTPGRRGLLYHRLISSQAPDFVSEPAEVQQALSTVSQAIAPLKGHKTVTWLLDSGF
                     DDVAVWRTIWEQDEHVVVRLYHTERTIAFQDRAGEWHEGDVAQATTQLRPLARVETSL
                     RVKRGKQARPKQQPVPVELSACPVRVSYWSNVRRPGDKGQLLTKEVWLVQVRVLGVDW
                     EPWLLLTDWPVTDAHSATRIFGMYRQRWSVEDAFKFLKTALGWEQVQLLDWRGIRTLL
                     ALGWVAAGFLFDLGVSFEWAEVQLIARLGGFEPHKDRVPGKLTLQRGLSRLLEMLTTQ
                     AFLSDYAAHHQGLPPRIAALLHGWQPPGEL"
     gene            complement(2363..>2597)
                     /locus_tag="VGT44_08165"
     CDS             complement(2363..>2597)
                     /locus_tag="VGT44_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580811.1"
                     /translation="APTRQMNLDYVVGADLSYSQPIMQIDKINRPPEQGRRGRPQGSP
                     WGLYTHVCILLSICMIGQSGIADRRGFAPINRL"
BASE COUNT          550 a          719 c          785 g          543 t
ORIGIN      
        1 gagtgatgtg ccctttttcg tctgaaccat ggatgacctc ctgtattgtg tggctctatg
       61 aataacctgt tttatagttt ttacaaaggt gtcctatcta caagggccga gagcaatgaa
      121 cgagttatgc gttatgcgtc aacccgctgg cgacgaggtt gatcagtaaa tccctcacac
      181 cggggtaggc atcttgacca atgggttctt tgtaaaaggt caggagcctg atgacggcga
      241 gtaggacgcc gacgatcgtc tcagggttgc catcaatcac gtacccagtc tcctgtccac
      301 gctggatgaa tggcctgatc ttgccaagtg agtcctgcag atgggcagca agctgttgag
      361 aggaaccctg gcgtatgagc aaggccaact cttcgggatg ggtcatcagg cgcctcgtca
      421 actcgcttcg ttccatctca tcgatgacca catgcataaa acggatgatc gcctcacgca
      481 catccgcggt ggtatcaaag gaagctgcca tgacctgctg ttccaagcgt gctcgctcct
      541 gcatcagcag ctctaagtaa aggtcctctt tcgactcgaa gaagaggtaa aaagtgcttt
      601 tggcaatccc caggggctgc gtcaaatctt caaggctagt tttcttcaac ccctggctga
      661 gaaacagcgt tcgtcccagc tctacgaggc gctgctgaag atgagctttc tcctcttcgc
      721 taaaagcacg agccatactc tatacctcta tgaacaatat atatttttgt ttttatctgt
      781 tgagtatacc ctcagaaacc actcctgtca agaggacagt gactgacatc cactcataaa
      841 tgggaaagag gtgatagaat caagggaaga cccagaatca acagaggagg aacgagagat
      901 ggagtggaac ccgacatggg aagacccctt gacgagcaac gtgaagcagt ttgatggatt
      961 gatgggagac cagcgcacgc gcaagacctt tggcgaagtg gtcaaaggca ttctggcggc
     1021 cggcagcctg atttgccaac agatcgcggc gcgctcggcg gagttaagcc gcgggaagaa
     1081 aggctcgcag cgtgtgatcc ggctggcgac cggagagagt acgcagcgtt ccgaactgga
     1141 tgccgagcat ctgacgcaac gcttgcggga ggtggcggtg gagcagttgg cccaggcgcc
     1201 ggaggacgaa ctgtggctga tcggcgatag ctccgacctg cgcaagccct acgcggaagt
     1261 gatgccgtat ctgatgccgg tgcgcgacct ggatgaccag ttggtgcccg gctaccgcac
     1321 gctcaatgtc ctcggcctga cccccggacg acgcggcttg ttatatcacc ggctcatcag
     1381 tagtcaggcc cccgactttg tgagtgagcc agcggaggtg cagcaggccc tgtccactgt
     1441 gagccaggcc atcgcgcccc tcaaagggca caagacggtg acctggctgc tggatagtgg
     1501 ctttgacgat gtggcggtgt ggcgcaccat ctgggagcag gacgagcatg tcgtggtgcg
     1561 cctttaccac accgagcgca cgatcgcctt ccaagatcga gcaggcgagt ggcatgaggg
     1621 cgacgtcgcg caagccacga cccagctacg tccactggcg cgcgtagaaa ccagcttgcg
     1681 ggtcaagcgc ggcaagcaag cgcggcccaa gcagcaaccg gtaccggtag agctatcggc
     1741 ctgcccggtg cgcgtctcgt actggagcaa tgtgcgccgg ccgggggaca aaggccagct
     1801 cctcaccaaa gaggtgtggc tggtgcaagt gcgggtgctg ggcgtggact gggagccgtg
     1861 gctgctgctg accgattggc cggtcaccga tgcccacagc gccacgcgca tctttggcat
     1921 gtatcgccaa cgctggagtg tggaggacgc tttcaaattt ttgaaaaccg cgctgggctg
     1981 ggaacaggtg caactgctcg attggcgcgg catccgcacc ttgctggccc taggctgggt
     2041 ggcggcaggc tttctctttg acctgggggt cagcttcgag tgggccgagg tgcaactcat
     2101 cgcccggctg ggcggctttg aaccccataa agatcgcgtg cccggcaaac tcaccttgca
     2161 gcgcggcctg agtcggctct tggagatgct gaccacccag gctttcctgt cggactatgc
     2221 cgcccatcat caggggctgc cccccaggat tgctgccttg ttacacggct ggcaacctcc
     2281 gggagagtta tgagtggatg tcagtcgctt gtaggcagga atggagcctc cccccctccc
     2341 cgtaggagcc gatttatcgt gctcacagcc gattgatcgg cgcgaatcct cgtcgatccg
     2401 caatgccgga ctggccgatc atgcagattg acaaaagaat gcagacatgg gtgtacaacc
     2461 cccagggcga cccttgcggt cgccctcgtc ttccctgctc tggtggccgg tttattttgt
     2521 caatctgcat gatcggctgt gagtacgata aatcggctcc tactacatag tcaaggttca
     2581 tttggcgtgt aggggcg
//
LOCUS       DASZAK010000468         4074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_117609, whole genome shotgun sequence.
ACCESSION   DASZAK010000468 DASZAK010000000
VERSION     DASZAK010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117609"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1622
                     /locus_tag="VGT44_08170"
     CDS             <1..1622
                     /locus_tag="VGT44_08170"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2580812.1"
                     /translation="WPYWSRSFVARAIAKGEYAMLIELRPLKRKDSGQIIAPLLSALG
                     QVIADYHQHPAYQEMLRNAHVSFDWIQYKSNFRPAIAERPALGIDAQDVHRRELQIDL
                     RQVNVETFPDELRRTLDRWKPPASPAEAPHDLPLPAVDGVGEADRAVAVAARPQTQAL
                     PDIDGDKLYLEPFQPARNSIIWSFNTLYWNALDKWEATFQKGYEAALPGGVTDACNPE
                     FVAESVKHVCEVLDELQRKGQLPDQIFVLEIGVGNGAQARTWLDAFQRYTSEARTDYY
                     QRLHYLMSDFSSCVLNAARDAVTPHLEKVSFLVMDATDPLKSLSFLRYKLMFVHISNV
                     YDNLPSSELLKWDNKYYQVEVRAYLPRAEAEKISEQYGQRNLGVTITRFLKIGPDYFD
                     DTGEGVHFWADVWRALKLEERCVLLPELSQLKLFEGFDGRDAHTLLDQVSGNVRMHLN
                     EMALKSFVNTVPLLHPRGFFQVQDIFITELEQYYSAFRGPGKYDGSVVNWVNGPLLRL
                     VGNRFGYDVHFQPFSYRQNSNIIILTTTVRD"
     gene            1624..2700
                     /locus_tag="VGT44_08175"
     CDS             1624..2700
                     /locus_tag="VGT44_08175"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580813.1"
                     /translation="MSDITTKREGQEPGERTRARERGDYMAIDKLLPTSVIGSYAVPE
                     WLERAKTDYLQRRISADSLNDMHNVVIKAAIKDQEMAGIDIVSDGELRRDNDIDYFAY
                     RIPGVEIDHRTKAYYYDFYDSVIRHKIPNASLGLLPDLEFLKQNTGRPVKFTITGPLT
                     LAKRIKNDYYQHEEEVALELARIMNGELRALAKAGADYIQIDESYLTGYPEDLAWVVR
                     AINIAVEGVESHIILHTCYGNRYGKPYWDGNYRFLFPAIFEAHIHQLALEFARKGDDD
                     LQLFSKFPSELELGLGVIDVKNQLVETPKLVAERIRRALKHLRPEKLYINPDCGLNHL
                     SGEIPFKKLCAMVQGTATVRKELS"
     gene            2697..4043
                     /locus_tag="VGT44_08180"
     CDS             2697..4043
                     /locus_tag="VGT44_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEV2580814.1"
                     /translation="MKGRRLPVLASEAGLGVGRTAGQVGKAAGNALLKSEEWSKGALF
                     DCHMCGICILQQTALICPMRCPKGMRNGPCGGPSLDGLCEVYPDKPCIWVEIYKRSER
                     FHLTKRVEQLQWPVDWSLQGTSAYGNVLNGKWFTPRWNQVLDQPKAEPRAGTNLERAL
                     DSGKFVVTAELGPPRSANAEFVRKRAELLRGKVAAVNITDNARGIARLSSLAGCLILQ
                     EMGIDAVMQMSCRDRNRVALQSDLLSAASLGIGNVLLLTGDHQRFGDDPQAMGVFDLD
                     GVSLVALARRMRDNGELLSGEKLPAPPRLLLGAAANPEGEPLDLQVLRLQKKVEAGAD
                     FIQTQAVFDIDHFQRWMAGVRARGLHKATKILVGILLLNSAERAEFLRKNVPGMRISE
                     TVVERLAKAKDPKAEGKLIGKELIQSLAEIDGVAGAHIMTIAWEDAIPEVLEGIYY"
BASE COUNT          866 a         1243 c         1245 g          720 t
ORIGIN      
        1 cctggcctta ctggagccgg tcgttcgtgg cgcgggcgat cgctaaggga gaatatgcta
       61 tgcttatcga actacggcct ctcaaacgca aagatagcgg gcagatcatc gcaccgcttt
      121 taagcgcgtt gggccaggta attgccgact accaccagca tccggcgtat caagagatgc
      181 tgcgcaacgc gcatgtctcg ttcgattgga tccaatacaa gagcaatttc cggccagcca
      241 tcgccgaacg tcccgcgctg gggatcgacg cccaggacgt gcatcgccgc gagttgcaga
      301 tcgacctgcg ccaggtcaac gtcgagacct tccccgacga gctgcgccgg acgctcgacc
      361 gctggaagcc gcccgcgtct ccggcggagg caccgcatga tctgccgctc ccggcggtcg
      421 atggtgtggg agaggccgac cgggccgtcg cggtggccgc caggccccag acgcaggcat
      481 tgcccgatat cgacggcgac aaactctatc ttgagccgtt ccaacctgcc cgcaacagta
      541 tcatctggtc gttcaacacg ctctactgga acgcgctcga taagtgggaa gctaccttcc
      601 agaagggcta cgaggcggcg ctgccgggcg gcgtgaccga tgcttgcaac cccgaattcg
      661 tggcggagtc ggtcaaacat gtgtgcgagg tgctggatga gctacagcgc aaggggcagt
      721 tgcccgacca gatcttcgtg ctggagatcg gcgtgggcaa cggcgcgcag gcgcgcacct
      781 ggctggatgc cttccagcgc tacaccagcg aggcgcgtac cgactactac cagcgcctgc
      841 attatctgat gagcgatttc tcatcctgcg tcctgaacgc cgcgcgcgat gccgtgactc
      901 cgcacctgga gaaggtcagc ttcctggtga tggatgccac cgaccccctc aaaagcctct
      961 cgttcctgcg ctataaactg atgttcgtgc atatctccaa cgtctatgat aacctgccaa
     1021 gcagcgaact gctcaagtgg gataacaagt attaccaggt cgaggtcaga gcctatctgc
     1081 cgcgtgcgga ggccgagaag atcagcgagc aatacggaca gcgcaacctg ggcgtgacca
     1141 tcacgcgctt cctcaagatc ggccccgatt acttcgacga cactggagag ggcgtacact
     1201 tctgggctga tgtctggcgc gcgctcaagc ttgaggaacg ctgtgttctg ctgccagaac
     1261 tgtcgcagct caaactgttt gaaggcttcg acggtcgcga cgcgcatacg ctgctcgacc
     1321 aggtcagcgg caatgtgcgc atgcacctca acgagatggc gctcaagagc ttcgtcaaca
     1381 cggttccgct gcttcacccg cgcggcttct ttcaggtgca ggacatcttc atcaccgaac
     1441 tggaacaata ctacagcgcc tttcgcggac ccggcaagta cgacggctcg gtggtcaatt
     1501 gggtcaacgg gccgctgctc cggctggtgg gcaatcgctt tggctatgac gtgcatttcc
     1561 agccgttctc ctatcgccag aactcgaaca ttatcatcct aacgacgact gtgagggact
     1621 gaaatgagcg atatcacgac aaagcgcgaa gggcaggaac ccggcgagcg caccagggca
     1681 agagaaaggg gcgattatat ggccatcgac aagcttctgc cgaccagcgt catcggcagc
     1741 tacgccgtac ccgaatggct ggaacgggcg aaaaccgatt acctgcagcg ccgcatcagc
     1801 gccgactccc tgaacgatat gcacaacgtg gtcatcaaag ccgccatcaa agaccaggag
     1861 atggccggga tcgatatcgt gagcgatggc gagctgcgcc gcgacaacga tatcgattac
     1921 ttcgcctatc gcatccccgg cgttgagatc gaccatcgca cgaaggcgta ttactacgat
     1981 ttttatgaca gcgtcatccg ccacaagatt ccgaacgctt cgctgggcct gctgcccgac
     2041 ctggaatttc tcaaacaaaa taccggcagg cccgtcaaat ttacgattac cgggccgctg
     2101 acgctggcca aacgcatcaa gaacgactat taccagcacg aggaggaggt tgcgcttgaa
     2161 ctcgcgcgca tcatgaacgg cgaattgcgg gcgcttgcta aagctggcgc ggactacatc
     2221 cagatcgacg agtcgtacct gaccggctac ccggaagacc tggcctgggt cgtgcgggcc
     2281 atcaacatcg cggtggaggg cgtcgagtcg cacatcatcc tgcacacctg ctacggcaac
     2341 cgctatggca aaccgtactg ggacggcaac tatcgttttc tcttcccggc catcttcgag
     2401 gcgcatatcc accagctcgc cctggaattc gcgcgcaaag gcgatgacga cctgcaattg
     2461 ttcagcaaat ttccttcgga actggagctg ggcttaggcg tgattgatgt caaaaaccag
     2521 ctggtcgaga cgccgaaact ggtcgcggag cgcatcaggc gcgcgctcaa gcacctgcga
     2581 ccggagaagc tctacatcaa cccggactgc ggcctgaacc acctgtcggg cgagattccc
     2641 ttcaagaagc tctgcgccat ggtgcagggg accgcgaccg tgcgcaagga actgtcatga
     2701 aagggcgacg tttaccggtg ctggcgagcg aggccggtct gggggtcggc aggaccgccg
     2761 gccaggtggg caaagcggcg ggaaacgcct tgctcaagag cgaggaatgg agcaagggcg
     2821 cgctcttcga ctgccacatg tgcggcattt gcattctcca gcagaccgcg ctgatctgcc
     2881 ccatgcgctg cccgaaaggg atgcgcaacg gcccctgcgg cggtccgtcg ctggatggtt
     2941 tgtgcgaggt ctaccccgac aagccgtgca tctgggtcga gatctacaaa cgctccgagc
     3001 gtttccacct gacgaagcgc gtcgaacaat tgcaatggcc ggtcgattgg tcgctccagg
     3061 gaacttcggc ctacggcaac gtcctcaacg gcaaatggtt cacccccaga tggaaccagg
     3121 tcttagacca gccgaaagcc gaaccgaggg ccgggacgaa cctggaaagg gcgctcgact
     3181 ccgggaagtt cgtggtgaca gccgaattgg gaccgccgcg ttcggccaac gccgagtttg
     3241 tgcgcaaacg cgccgaactg ctgcgcggga aagtcgcggc ggtcaacatc accgacaacg
     3301 ccaggggcat cgcccggctc tccagcctgg ccggctgcct gatcctgcaa gagatgggca
     3361 tcgacgcggt catgcagatg agctgccgcg accgcaaccg cgtggcgctg caaagcgacc
     3421 tgctcagcgc ggccagcctg ggcatcggca atgtgctgct cctgacgggc gatcaccagc
     3481 gcttcggcga cgacccgcag gccatgggcg ttttcgacct ggacggcgtc tccctggtgg
     3541 cgctggctcg caggatgcgc gataacggcg aactgctctc aggcgagaag ctgcccgcgc
     3601 cgccgcgcct gctgctgggc gcagccgcca acccggaggg cgagccgcta gacctgcaag
     3661 tcctgcgcct gcaaaagaag gtagaggcgg gcgcggactt catccagacg caggccgtct
     3721 tcgacatcga ccactttcaa cgatggatgg ctggtgtacg ggcgcgcggc ctgcacaagg
     3781 cgacgaagat cctggtcggc atcctgctct tgaactcggc ggaacgcgcc gagttcctgc
     3841 ggaaaaatgt gccgggaatg cgcatctccg agacggtcgt agagcggctg gcaaaggcaa
     3901 aagacccgaa ggcggaaggc aagctgatcg gcaaggaact gatccaatcg ctggctgaaa
     3961 tcgatggcgt ggctggcgcg catatcatga cgatcgcctg ggaagacgct attccagaag
     4021 tactcgaagg tatatactac taatgcgtca aggttcacgt gttgtgtagg ggcg
//
LOCUS       DASZAK010000469         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_83019, whole genome shotgun sequence.
ACCESSION   DASZAK010000469 DASZAK010000000
VERSION     DASZAK010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83019"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..581)
                     /locus_tag="VGT44_08185"
     CDS             complement(15..581)
                     /locus_tag="VGT44_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV2580815.1"
                     /translation="MIGPFNWIDLLFLVTVVLLVFNGLRNGAIFSLVNLLSIPVALGV
                     ALYFGQPFTAFLASNGISISPLIAYIILFFVAVILIHILGTVLRGVIKAIPLVGLGDA
                     LIGGAIGFVEAWLLWLIVLLVVGNVLHGVQNSFLAGTHLFSGINLTPQQLQTWHDAYN
                     QAITTSLFAKVNGFFVQALPALPKLQSV"
     gene            complement(840..1517)
                     /locus_tag="VGT44_08190"
     CDS             complement(840..1517)
                     /locus_tag="VGT44_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580816.1"
                     /translation="MSAMAIRILLCEDQTLMRQGLHTVLELEPGFEVVGEAANGEEAI
                     ARYAELRAQGKGPDIVLMDVQMPRKNGVQATAAITTAHPGARVIILTTFDYEDYVFDA
                     IKAGAMGYLLKDVPADQLATTIRSVHGGEPFIQPNVASKLLVEFGRKGRSLSAQGISP
                     ADEELSSREIEVLKLLAGGASNRDIADRLALAEGTVKNHVSNILSKLHAENRTQAANL
                     ARERNII"
     gene            complement(1514..2101)
                     /locus_tag="VGT44_08195"
     CDS             complement(1514..2101)
                     /locus_tag="VGT44_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2580817.1"
                     /translation="MTSNDLQQLKMAWLAAEDAGDKQAQIALLRDHPDAQDELIDFIA
                     VYHMSAPVGSQTALLPLTQRAISRAFEHVFSPQSVVAAANLRDLRAKQGLTLVQAARG
                     LRLGIDVWKKFEDGVIDLVSLSERHLERLARFFHISAEQFGSLLLQSQPLPTLDRRQT
                     AQAARSSQQSRLPKQPFAEAIEKSAMTREEKEEWR"
BASE COUNT          443 a          619 c          689 g          437 t
ORIGIN      
        1 gtcttgacag gctatcagac actctgcaat ttcggcaagg cgggcagtgc ctgcacgaag
       61 aagccgttga ccttcgcgaa gaggctggtg gtgatggcct ggttataggc gtcatgccag
      121 gtctggagct gttgaggcgt caggttgatg cccgaaaaga ggtgggtgcc ggccaggaac
      181 gaattctgca ccccgtgcag gacgttgccg accaccagca ggacaatcag ccacagcagc
      241 cacgcctcca caaagccaat cgccccgccg atcagcgcgt cgcccaggcc gactaagggg
      301 atagctttga tgacgccccg caggacggtt cccaggatat ggatcaggat cacagccacg
      361 aaaaacagga taatgtaggc gatgagcggc gaaatgctga tgccgttcga ggccaggaag
      421 gcggtgaagg gctggccgaa gtagagcgcc acgcccaacg caaccgggat actgagcaga
      481 tttacaaggc taaaaatcgc gccattacgc agaccattaa agacaagcaa tacaacagtg
      541 acgagaaaca gaaggtctat ccagttaaag ggccctatca tagcatggtt cctccaatgc
      601 gtatccacga cctatattca gcagatgtgt ttcatatctt gatagacgtt gcgcgaagga
      661 tagttgtaac aagaatagag gcaaatgtga aagggcgacc gcaagggtgc gcctttccag
      721 caaattccca aagggcgacc gcaagggtgc gcccctacca tgactacgaa gaggttaggc
      781 gcatcgccac tcgccgggtc atggtaaggg cgcacccttg cggtcgccct gaggtttggc
      841 taaataatat ttcgctcgcg cgcgagattg gcggcctggg tgcgattttc ggcgtggagc
      901 ttactcaaaa tattggagac gtggttcttg acggtgcctt cggccagcgc cagccggtcg
      961 gcgatatcgc gattgctggc gccgccggcc aggagcttga gaacttcgat ctcgcggctg
     1021 ctcagttctt catcggcggg gctgatgccc tgcgcggata aggagcgccc tttgcgccca
     1081 aattcgacga gcagtttgct ggcgacgttg ggctggatga acggctcgcc gccatgcacg
     1141 ctgcgaatgg tggtggcgag ctggtcggcg ggtacgtctt tgagcaggta gcccatcgcc
     1201 ccggctttaa tggcatcgaa gacataatct tcgtaatcga aggtggtgag gatgatgacg
     1261 cgcgcgccag gatgcgccgt ggtgatggcg gcggtggcct gcacgccgtt cttgcgcggc
     1321 atctgcacat ccatcaggac gatatccggc cctttgccct gcgcgcgcag ttcggcatag
     1381 cgcgcgatgg cctcttcgcc gttcgcggcc tcgcccacaa cctcgaagcc tggttccagt
     1441 tccagcacgg tatgcaggcc ctggcgcatg agcgtctggt cttcgcagag caggatgcgg
     1501 atggccattg cgctcatcgc cactcctctt tctcctccct ggtcatcgcg ctcttctcga
     1561 tggcctcggc gaaaggctgt tttggcagcc gcgactgctg actgctgcgc gcggcctgcg
     1621 cggtctggcg gcggtccagc gtgggcagcg gctggctctg gagcaggagg ctgccgaact
     1681 gctcggcgga gatgtggaag aaacgcgcca ggcgttccag gtggcgctca ctgaggctga
     1741 ccaggtcgat cacgccatcc tcaaactttt tccagacatc gatgcccagg cgcagcccgc
     1801 gcgccgcctg cacgagcgtc aggccctgtt tcgcgcgcaa gtcgcgcagg ttcgcggcgg
     1861 caaccacgct ctgaggagag aagacatgtt caaacgcgcg ggagatggcg cgctgtgtca
     1921 aaggcagaag agccgtttgg gagccgacag gggcgctcat gtgatagacg gcgatgaaat
     1981 cgatcaattc atcctgcgcg tcgggatggt ctcgcaggag agctatctgc gcctgtttat
     2041 ctccggcatc ttcggcggcc aaccaggcca tttttagctg ctgtaaatcg ttgctcgtca
     2101 tgatacgttt ccttcttgcc atcaaagcat gtgttttgcg tgtgaatatg ccgcctgttc
     2161 ctgtgccagg gacgcggcca ggtagctg
//
LOCUS       DASZAK010000470         5025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_83042, whole genome shotgun sequence.
ACCESSION   DASZAK010000470 DASZAK010000000
VERSION     DASZAK010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83042"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VGT44_08200"
     CDS             <1..802
                     /locus_tag="VGT44_08200"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEV2580818.1"
                     /translation="EQRDFLKALAKVGATTAIASNEVARLANRLYGTVFDSKALPGKV
                     LAPLEKAGFLTTEKSTEGRGAKPHAVQPTEQFTKEVLLPLLRASSKGLDARLREMLYR
                     PLSEIIDELDSTDKNSKGKALEALVLRITRLLDLQFVGWRLRQRRTGGAEVDVLVESD
                     RLIFSRWQIQCKNTHRVELEDIAKEVGLTFYLKSNVIMIVTTGKIGKPARDYATGIIE
                     ATNLHIILLDGKDLARLRVQPSAIVEILDREAKNAMQLKELKVPLEEI"
     gene            802..1881
                     /locus_tag="VGT44_08205"
     CDS             802..1881
                     /locus_tag="VGT44_08205"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV2580819.1"
                     /translation="MTENLFHFTDSTYLYRTPYGQAYLGDSREYLRGFPDNSVDLIMT
                     SPPFALRRKKEYGNVSADEYIEWFMPFANEFYRVLKPEGSFVVDIGGSWNPGEPTRSL
                     YHYELLIALCRRSGFHLAQEFFWYNPARLPTPAEWVTVRRVRVKDAVNTIWWLSKTPH
                     PKANNRKVLREYSDSMRELLVRGYKPKLRPSGHDISDKFQRDNGGAIPPNLIELSNTE
                     SNSFYLTACKQANIKPHPARFPVALPDFFIKFLTDQDDLVVDPFGGSNSTGEAAEALQ
                     RHWITFELVSDYLEGSKFRFPELQPTHLLWAGITQERPAEYVIDPAHELVDATEDIAE
                     ESIEGEEAALIEIRNSTIIMDEVHE"
     gene            2065..4458
                     /locus_tag="VGT44_08210"
     CDS             2065..4458
                     /locus_tag="VGT44_08210"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV2580820.1"
                     /translation="MASEIRPVVETIDQTPVEPLDVEVDRAIFALEGMTCASCAMRIE
                     KGLKKVPGVLDASVNLATELANVTYDPQQTGIEQMVQKVEAVGYKAYPHIAQLPKVEQ
                     QDCAAGVVQDETGTTGASAQVDELSERKQAEITRKRNLLLLGIVLTLPVVILSMFFMN
                     RFPGENLLLLVLASPVWAIVGWEFHRGALKTLRHGSANMDTLISVGSTAAYVMSVVAT
                     FLPQIVGGVTFFDTTALIVTLIFLGKYLEARAKGQTNDAIKKLIGLQARTAHVLRDGL
                     EVELPIEQVQVNDVLVVRPGEKVPVDGTILSGLSTVDESMITGESIPVEKAEGDTLIG
                     ATINQRGLLRMRATKVGADTVLANIIRMVEQAQGSKAPIQRLADTISSIFVPVVLVIA
                     SLTFIGWAIAGNVLAGSFTANSMPGSTNAWIVAIVAAVTVLVVACPCALGLATPTAIM
                     VGTGKGAEQGILIRGGESLERIQAVRAVLLDKTGTITKGKPSLTDVVALPGWQEDEAL
                     RLVAQAEQGSEHPLAAAIVEGAKARGITLTHTPERFEAIAGRGLVANVEGHSVLVGTR
                     RLLNERSIAYESIEEQLAALEQQGKTAMIAVIDGQVAGIVAVADTVKFGSAEAVKQLQ
                     AQGQSVWMITGDNRRAARAIAAQVGIPAEHVLAEVLPEEKASQVKRLQAQGLVVAFAG
                     DGINDAPALAQADAGIAMGTGTDIAMEASDITLVKGNLKSVATALALSRATMRTIKQN
                     LFWAFAYNVILIPTAILSPLIPFLQTNAPIFAAAAMALSSVTVVGNSLRLRRFGRQV"
     gene            4517..4786
                     /locus_tag="VGT44_08215"
     CDS             4517..4786
                     /locus_tag="VGT44_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEV2580821.1"
                     /translation="MEAERKPEVLKRLAYIEGHVTGIRKMVEEDKYCIDVLRQTYAVR
                     KSLEKLESLILEGHLRTCVPEGIKGDREEQVIQELIQLYTIAGNQ"
     gene            4834..>5025
                     /locus_tag="VGT44_08220"
     CDS             4834..>5025
                     /locus_tag="VGT44_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEV2580822.1"
                     /translation="MLESIDLVAPDISCEHCQHAIEGAVGSMEGVSQVKVDIPTKTVH
                     VAYDPQHVTLAKIEEVLDDT"
BASE COUNT         1207 a         1246 c         1455 g         1117 t
ORIGIN      
        1 ggaacagcga gatttcctca aagccctggc gaaagtaggc gctaccaccg ctatagcgtc
       61 taatgaagtt gctcgactgg cgaaccgtct ctatggaact gtcttcgatt ccaaagctct
      121 gccaggtaaa gttttggcac ctttagaaaa agcaggtttc ttaacgactg agaaaagcac
      181 ggagggccga ggtgcaaagc ctcatgcggt ccaaccaacg gagcaattta cgaaggaggt
      241 ccttctacct ctgttaagag catcctcgaa agggctggat gcgcgtctgc gagaaatgct
      301 ctatagacct ttgtcagaaa ttattgacga attagacagt acagataaga atagcaaagg
      361 gaaggcgtta gaagctcttg ttttgcgtat cactcgtctg ctcgatcttc aattcgttgg
      421 atggcgactc agacagagac gcacgggagg agcagaagta gacgttttag tagaaagcga
      481 ccgcctcatt tttagcaggt ggcaaataca gtgtaaaaac acgcatcgcg ttgagctaga
      541 ggacatcgca aaagaggttg gactcacttt ttatctgaaa tccaacgtta ttatgattgt
      601 tacgaccggc aaaatcggaa agccagcgcg cgattatgca acaggcataa ttgaggcgac
      661 gaatttgcac ataattctac tggatgggaa agatctagcg cggttgcgcg ttcaaccgtc
      721 agccattgta gaaatccttg accgggaagc aaagaatgca atgcaattga aggaactcaa
      781 ggttccatta gaggaaattt gatgactgaa aatttgtttc atttcacaga ttcaacgtat
      841 ctatatcgga caccttatgg tcaagcatat ctgggagact cacgtgaata cctgcgcgga
      901 tttccagata acagtgttga tctgattatg acttcgcccc catttgctct tcgtcgcaag
      961 aaagaatatg gcaatgtcag cgcagatgag tatatcgagt ggttcatgcc tttcgctaat
     1021 gagttctatc gtgttctcaa gcctgagggt agtttcgtgg tagatattgg cggtagctgg
     1081 aatccaggtg aaccaacgcg gtctctctat cattatgaac ttctgatagc gttatgtcga
     1141 agatcgggtt ttcaccttgc tcaggaattt ttctggtata atcctgcccg gttgcctaca
     1201 cctgccgaat gggtgacggt tcgacgtgta cgagtcaagg atgcagtaaa taccatctgg
     1261 tggctctcta agactcctca tccaaaagcg aataatcgta aggtgctaag agaatacagc
     1321 gattctatgc gggagctgct ggtacgcggg tacaaaccga aattgcgccc ctctggacat
     1381 gatatttcgg acaaatttca gcgtgacaat ggaggcgcaa ttcctccaaa cttaatcgag
     1441 ttaagtaaca cagaatcgaa tagcttttat ttaacagcct gcaaacaagc gaatattaaa
     1501 cctcaccccg ctcgtttccc ggtagcgctg ccagatttct tcatcaaatt cctgacagac
     1561 caggatgatc ttgtggtcga tccatttggt ggcagcaatt cgactggaga ggcagcagaa
     1621 gctcttcagc gccattggat cacctttgaa ctggtttcag actacctgga aggttcaaag
     1681 tttcgttttc ccgaattaca accgacacat ttattgtggg caggcatcac ccaggagcgt
     1741 ccagcagagt acgttatcga tcctgctcat gaacttgttg atgccacaga ggatatagcg
     1801 gaggagtcca tagaggggga agaagcggct cttatcgaga ttcgtaatag cactatcatc
     1861 atggatgaag ttcatgaata agggtgggaa ggaattgcac cttcgcgccg ttggtcgcta
     1921 agcagacgac agcctacctc cctgcaacat gtaggtcaaa gacctgtgac ctttttcctt
     1981 gacatccttt acctttgtct agtatacttt aaataccccc taccgggggg tatgggtcag
     2041 acggtactag ataaggcgat gacaatggct tccgaaattc ggcctgttgt agaaacaata
     2101 gatcaaacgc cggttgagcc tctggatgta gaggtagacc gggcgatttt tgcattagag
     2161 ggtatgactt gcgcctcgtg cgcgatgcgt atcgaaaaag gtctcaagaa ggttcccggt
     2221 gtgctggacg ctagcgtgaa cctggcgacc gagctggcaa acgtcacgta tgacccgcag
     2281 cagacgggca tcgagcagat ggtgcagaag gtcgaggcgg tcggctataa agcgtatccg
     2341 cacattgcgc agctgccgaa agtggaacaa caggactgcg cagccggtgt cgttcaagac
     2401 gagaccggga cgactggcgc ttctgcccag gtagatgagt tgagcgaacg caagcaggcg
     2461 gagattacac gcaagcgcaa cctgttgctg ctgggcattg tcttgacgct gccagtagtg
     2521 atcctgagta tgtttttcat gaaccgcttc cccggagaga acctgctgct gctggtgctg
     2581 gcatcacccg tatgggccat cgtcggatgg gagtttcatc gcggcgcgct caagacgctg
     2641 cgacatggca gcgccaacat ggatacgctc atctcggtcg gctcgaccgc cgcgtatgtg
     2701 atgagcgtgg ttgccacttt cttaccgcag attgttggag gtgtgacatt cttcgatacg
     2761 accgccctga tcgtgacgtt gattttcctg ggcaagtacc tggaagcgcg cgccaagggg
     2821 cagaccaacg acgccattaa gaagctgatc ggcttgcagg cgcgcacagc gcacgtgctg
     2881 cgagatggac ttgaagtaga gctgccgatt gagcaggtgc aggtcaacga tgtgctggtg
     2941 gtgcggcccg gcgaaaaagt tcccgtcgat ggcacgattc tgtccggcct ttcgacggtc
     3001 gatgagtcga tgattaccgg agagagtatt ccagttgaaa aggccgaggg agatacgttg
     3061 atcggggcga ccatcaacca gcgcgggctg ctgcggatgc gcgccacgaa ggtcggagcc
     3121 gataccgtgc tggccaacat catccgtatg gtcgagcagg cgcagggatc gaaagcgccc
     3181 atccaacgtt tagccgatac tatctccagc atcttcgtgc cggtagtgct ggtgatcgcc
     3241 tcgctgacgt tcatcggctg ggctattgca ggcaacgtcc tggccgggag tttcacagcg
     3301 aacagtatgc ctggttcgac caatgcctgg atcgtggcga ttgtcgcggc tgtgaccgtg
     3361 ctggtcgtgg cctgcccgtg cgcgttggga ctggcgacgc cgaccgccat catggtcggc
     3421 accggcaagg gcgcggaaca gggcatcctc attcgcggcg gtgaaagcct ggaacgcatc
     3481 caggccgtgc gcgccgtgtt gctcgataag accggcacca tcacgaaggg caaaccgtcg
     3541 ttgaccgatg tagttgcgct tcctggatgg caagaagacg aggcgctgcg cctggtggcc
     3601 caggccgagc aaggttcgga gcatcccctg gctgcggcga ttgtcgaggg cgcgaaggcg
     3661 cgcggcatca cactcacaca tactcccgaa cgcttcgagg ctatcgccgg gcgtgggtta
     3721 gtggctaacg tagaaggaca cagcgtgctg gtcggtacgc gccgcctgtt gaacgagcga
     3781 tccatcgcat atgagtccat cgaagagcag ttggccgctc ttgagcagca gggtaagacg
     3841 gccatgatcg cggtgatcga cggccaggta gcagggatcg tcgcggtggc cgataccgtc
     3901 aagtttggct cggcggaggc tgtcaagcag ttgcaggcgc agggccagtc ggtctggatg
     3961 ataaccggcg acaaccggcg agcagcgcgg gcgattgccg cgcaggtggg catccccgcc
     4021 gaacatgtcc ttgccgaagt gctgccagag gaaaaggcca gccaggtcaa gcgtttgcag
     4081 gcacagggat tggtggtcgc tttcgccggt gatggcatca acgatgcccc ggcgctggcg
     4141 caggcagatg ccggtatcgc gatggggacg ggtaccgata tcgcgatgga ggcatcggac
     4201 atcacgctgg tcaagggcaa tttgaaaagt gtcgcgacgg cgctcgcgct ttcgcgggcg
     4261 acaatgcgca ccatcaagca aaacctgttc tgggcctttg cctataatgt cattctcatt
     4321 ccaaccgcga tcctttcgcc tctcattccg ttcttgcaaa cgaacgcccc cattttcgcg
     4381 gctgcggcga tggcgctctc ttcggtgacg gtcgtcggca attccttgcg cctgcgccgc
     4441 tttgggcgac aggtgtagag agcggattga gtcaaaacac aagcagattg agccatgcag
     4501 tcaaaggaga cgtaagatgg aagcagagcg caaaccggag gtattaaaac gcctcgcgta
     4561 tatcgagggc catgtgacgg gtattcgtaa gatggtcgag gaggataaat actgtatcga
     4621 tgtcttacgt cagacctatg cggtacgcaa atctttggaa aaactggaat ccctgattct
     4681 cgaagggcat ttgcgtacct gcgtccctga aggaatcaag ggcgatcgcg aggaacaggt
     4741 catccaggag ttgatccagc tctacacaat tgccgggaac cagtaggttt ttaaccaggc
     4801 gctatgcgca taaatcaaga aggagaaaat ttcatgttag agtcaatcga tcttgtcgct
     4861 cccgatatct cgtgcgaaca ttgccagcac gccattgaag gggccgtagg cagtatggag
     4921 ggcgtcagcc aggtgaaggt tgatatccca accaaaaccg tgcatgtcgc ttacgacccg
     4981 cagcatgtga ccctggcgaa gattgaggaa gtcctcgacg acacc
//
LOCUS       DASZAK010000471         5175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_83054, whole genome shotgun sequence.
ACCESSION   DASZAK010000471 DASZAK010000000
VERSION     DASZAK010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83054"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VGT44_08225"
     CDS             <1..267
                     /locus_tag="VGT44_08225"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580823.1"
                     /translation="IWDAKTAATIATYNGHSSPVLAVAWSPDSTHIASIGHNDNKVQV
                     WSVAKGSCTSIYDRHGSDVFAVAWSPDGTKIASAGYGVHIWQAS"
     gene            complement(408..710)
                     /locus_tag="VGT44_08230"
     CDS             complement(408..710)
                     /locus_tag="VGT44_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580824.1"
                     /translation="MIDSPVTFIENPPGQPAAVPEKKEWPPIMRRLSLAGVVLVSIFM
                     NFYQLGQNGFANLYYAAAIRSMLDSWHTRGLPVVGTESRNSNQYTPKRPCSPDEKS"
     gene            1208..3091
                     /locus_tag="VGT44_08235"
     CDS             1208..3091
                     /locus_tag="VGT44_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580825.1"
                     /translation="MAHFGRGGFSGMGGGGMGGMATLWNQQRSLQYLSEGKPAIARTL
                     RLVWQALRVYRWQLMLGTGVTVLGVGVGLIPPLLIRELIDYAIPQRQLSLILLLGSGL
                     LLFPIGGALLGLGQNYLSITIAQGLIADLRERLYRHVQSLGLEFFTWTRAGEIHTRFL
                     NDAGGLQNVLTQSFIGTFANLITLIGSLAIMATIDWRLALVTAVALPAFAFPIVYFGK
                     QRYAAMERAQAALGELSVALEETLSLSGAIVVKSFGTEEREARRFNEVNTQVRKEQVG
                     QLVVGQWLSVVVQSLAALGPALLYTYGAYLVITHQVAIGTIVAFATYLAQLYAPASSL
                     AGANATLLGGLALFDRVFLYLDIPASVPEPAEPQTLPPQPKEGITFEQVGFTYPGKAS
                     NKEVLHDVSFIAPLGQLTALVGPSGAGKSTILSLAARFYGPTSGAVRLDGVALPEIAR
                     GDLRRRLAVVTQELFLFHASLQENICYGLPDANRADVVAVIEAAQLQDLIDRLPDGLD
                     TVVGERGYRLSGGEKQRVAIARALLCQAPYLLLDEATSSLDSQVERKIQAALEKLVEG
                     RTVIAIAHRLSTIQRADQILVVQHGSIVERGTHAGLLQQSGLYQRLYAAQFAPDQARV
                     TNL"
     gene            complement(3775..>5175)
                     /locus_tag="VGT44_08240"
     CDS             complement(3775..>5175)
                     /locus_tag="VGT44_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580826.1"
                     /translation="KLTLRQRLRELTSPAWLTRDMRLLLAARVSMSAARALAGIVVPI
                     YLALIGFNALELGFLFVVVAIMSAVLSGLSGLLADRVGRKPMIVLLPFCTALAAIIFV
                     YSHVAALLFVFAALGSFGRGAGAGAGAVGPYQPAEQALLADAVSSRYRNNLFGLVGFA
                     SSLGALLGTGPLTSLPQVLTHFSILSSQGLASYQFAFFLTAIAAAIAGLLAIPVADPS
                     LTRIRNRRAMNDTSAQTIKRKGLWRINISHQSMSILLRLCITNSFNGLAVGFFGPFIT
                     YWFYRQYGAGPAVIGLLYSIINLAAMFANLGSARFAARLGLVRAILVGRIFQAVLLIP
                     MVLAPTFWLAGAIYLARMLAQRLALPLRQSYVMGVVSPEERGTVSALSNLPAQATSAA
                     SPALAGYLFDHISLALPFEIGAALQAFNALLFFIFFRNNLPPEERDARSLRENAETLE
                     SQADEAEKISSQFNGV"
BASE COUNT         1140 a         1451 c         1524 g         1060 t
ORIGIN      
        1 atttgggatg ccaagaccgc agcgaccata gccacttaca atggacactc ttcacctgtg
       61 ttggcagtcg cttggtcgcc tgatagtaca cacattgctt ctatcggcca taatgataat
      121 aaggtgcagg tctggagtgt agctaaagga agttgcacat ctatatatga tagacatggt
      181 tcagatgtgt ttgcagttgc ttggtcgcct gatgggacaa agattgcctc tgccggctat
      241 ggagtacata tctggcaagc atcatgattt caaataacaa tatatagcct ctccgtaaat
      301 cagaggtata acaactttgc gctatatgac ctcaccctcc ccgaaagcaa tgcccaaggt
      361 catcgcagcc ccttcgattc ccttgttctg ctactcaaga gctttttcta acttttttcg
      421 tcaggagaac acggtctttt tggagtatat tgattgctat tccggctttc tgttccaacc
      481 acgggcaaac ctcgagtatg ccagctatcc agcatacttc tgatagccgc tgcatagtag
      541 agattagcaa agccattctg ccccaattgg taaaagttca taaagatcga gaccagcaca
      601 accccggcca gcgatagacg ccgcatgatg ggcggccatt cctttttttc cggcacggca
      661 gcaggttgcc ccggcggatt ctcaataaac gttacgggcg aatcaatcat tacgtttata
      721 ttcctttcaa ttacagtcga tttctgaggg cgaatagtcg ggccttctgc acagggtggt
      781 tcaccttcgt ccttcacagc gtatcagaac aagatgggaa ttgaattgga ggaggatgag
      841 aaaaatctca tggcggctct gcaattcaca tgatcgcgca cagccaacaa tgtactttca
      901 caatcaagat cggcaaatga gcctgtcatc cttcgctgcg ctcaggatcg cggtcccagg
      961 cgcgtcagat ccttcgctgc gctcaggatg acattaccga tgttgatcgt gaaacttcat
     1021 tatcggcctg tccaaaccaa tgatgagtga ttcgcgccga tcaatcggct ccttcgaaat
     1081 attgccgatg ctggtcgtga aagttcaaca ttgaccattg ttttcgtctg tgctatgctt
     1141 tgcggtagat cgttaactag gttagttaat cagatggtga ctggcattct gtctggaggt
     1201 ctgcgctatg gcacattttg gtaggggagg ctttagtggc atgggtggcg gggggatggg
     1261 aggaatggcc acgctctgga atcagcagcg atcattgcaa tatttaagcg aggggaaacc
     1321 tgctattgct cgcacattgc gcctggtctg gcaggcgctg cgcgtctatc gctggcaact
     1381 gatgctggga acaggggtga cggtgctggg cgtcggcgtc gggctgattc caccgctgct
     1441 gatacgcgaa ctgatcgact acgccattcc acagcgccag ttgtcgttga tcctgctgct
     1501 gggcagcggc ctgttgctct ttcccatcgg gggggcgctg ctcggcctgg gccagaacta
     1561 cctgagcatc accatcgcgc aggggctgat cgctgacctg cgcgagcggc tctaccggca
     1621 cgtgcagtca ctcggcctgg aatttttcac ctggacgcgc gcaggggaaa tccatacgcg
     1681 cttcttgaac gatgcgggag ggctgcaaaa cgtgctgacg cagtccttca tcggcacctt
     1741 cgcgaacctg attaccctga tcggctcgct ggcgatcatg gcaaccatcg actggcggtt
     1801 ggcgcttgtc accgccgtag cattaccggc ctttgcgttt ccgattgtgt actttggcaa
     1861 gcagcgctac gcggcgatgg aacgcgcgca agccgcgctc ggtgagcttt cggtcgcgct
     1921 ggaagaaacg ctcagcctgt cgggggcgat tgtcgtcaag agctttggga cggaagaacg
     1981 cgaggcccgg cgtttcaacg aagtcaatac acaggtgcgc aaggagcagg ttgggcaact
     2041 cgtggtagga caatggctct cggtcgtggt gcagagcctg gccgcgctcg gacccgccct
     2101 gctttacacc tacggcgcgt atctcgtgat tacgcaccag gtcgcgatcg gcacaatcgt
     2161 ggcctttgcc acctacctgg cgcagctcta cgccccggcg tcctcgctgg cgggcgcgaa
     2221 cgcgacactg ctgggcggct tagcgctgtt cgatcgcgtc ttcctctatc tcgatatccc
     2281 cgccagcgta cccgaaccgg cagaaccgca aaccctgccg ccacagccga aagagggcat
     2341 cacatttgag caggtcggct ttacctatcc cggcaaagct agcaataaag aggttctgca
     2401 tgacgtctca tttatcgcgc cgctggggca acttactgcg ctggtagggc ctagcggagc
     2461 gggcaaatcg accattctct cgctggcggc gcgcttctat ggccccacga gcggcgctgt
     2521 gcggctggat ggcgtcgcgc tgccggagat agcgagaggc gacctgcgcc gccgcctggc
     2581 ggtcgtcacc caggaactct tcctgttcca tgccagcctg caagagaata tctgctatgg
     2641 ccttcccgac gcgaacaggg ccgatgtcgt ggccgtgatc gaggccgcgc agctccagga
     2701 tttgatcgac aggctgcccg acggcctcga tacggttgtc ggagagcgcg gctaccggct
     2761 ctccggcggc gaaaaacagc gcgtggctat cgcgcgggcg ctgctgtgcc aggcgcccta
     2821 cctgctgctg gatgaggcca ccagttcgct cgactcgcaa gttgagcgga agattcaagc
     2881 cgcgctggaa aaactggtcg agggcaggac agttatcgcc atcgcccatc gcctctcgac
     2941 gattcaacgc gccgaccaga ttctggttgt gcagcatggg agcatcgtcg agcgtggtac
     3001 ccacgcagga ctgttgcaac agtcggggtt ataccagcgg ctctacgccg cgcaattcgc
     3061 accggaccag gcaagagtaa ccaacttgta ggcgaagggt ttccacgcat atcgccgaat
     3121 acactcaagg aaaatgcaca aacggcagat atcgatgtga gggaaaggga atcggcatat
     3181 gaaacgatca ggtacgctgc gatatctctt ctggctggta ggctttaccg tgctcctggc
     3241 cgggtgtagt acatcatccc tactcaaaac gccctggtct cgccatcacc atcccaacca
     3301 gcaagactac tcaagccaga tgctcctatt ccatcctcct gcccggcaac acctgtacat
     3361 atcagagggc catatggaag cgaatctccg tggctccagg ctcaaccgac ctcatcgggc
     3421 atcgttgcat accttgctta ttccggcgtg ggcccatccg ggacataccg gcttccccat
     3481 tccaacggct tcttccctgg agaaggattg tacaccaaga ccctctgggc tatcgacaac
     3541 ggccaggctt ctagcacgtt tctgatcgat ggaacatatc tttcagatgt taccaggact
     3601 tttcatgaca ctggaaacga gatgtggggt tctgggcttc caaccagtct caacatgcat
     3661 acgtatacat cctacttgaa ggcccccagc gtgggctgct ggcgcttgca gatcaccgct
     3721 ggtcaggcca gaggcgacat cactatgtgg ttcatgggat aaccagccct aacattagac
     3781 tccattgaac tgagaagaga ttttctcggc ttcatctgct tgagactcga gcgtctcggc
     3841 gttctcgcgg agactgcgcg catcgcgttc ttcagggggc aagttgttac gaaagaagat
     3901 gaagaagagc agagcgttga aggcttgcag cgcggcaccg atctcgaagg gcagggcgag
     3961 tgagatgtga tcgaagaggt agccagccag agcgggactg gccgccgagg tagcttgcgc
     4021 agggaggttg gagagcgcgc tgacggtgcc gcgttcttcg ggcgagacga cgcccatcac
     4081 gtaggattga cgcagaggca gcgccaggcg ttgagcaagc atacgcgcca ggtagatagc
     4141 gcccgctagc cagaaagtcg gcgcgagaac catcgggatg agcagcacgg cctggaatat
     4201 gcgcccgacg aggatggcgc gcaccaggcc caggcgcgcc gcgaaacggg cagaacccag
     4261 gttggcgaac atggccgcga ggttgataat cgaatagagc aggccgatca cggcaggccc
     4321 ggcaccgtat tggcgataga accagtaggt gatgaatggg ccaaaaaagc cgactgccag
     4381 gccattgaag ctattcgtaa tgcagaggcg cagcagaatc gacatcgact ggtgggagat
     4441 gttgatgcgc catagtccct tgcgtttgat cgtctgcgcg ctcgtgtcat tcatggcgcg
     4501 ccggttcctg atgcgtgtaa gcgaggggtc ggcaacgggg atggccagca gaccggcgat
     4561 ggctgcagcg atagcggtca ggaaaaaggc gaactggtag gaggccagac cctgagagct
     4621 gagtatgctg aagtgggtca acacctgtgg caggctggtg agtggtccgg ttccgagcag
     4681 cgctcccaac gaggaggcga agccgaccag cccgaagagg ttgttacgat agcgcgatga
     4741 gaccgcgtca gcgagcagcg cctgttcagc cggttgataa gggccaaccg cacccgcgcc
     4801 cgcgcccgcg ccgcgaccaa acgagccaag cgccgcgaac acgaagagca gagcagctac
     4861 atgcgaatag acgaagatga tggctgcaag cgccgtgcaa aagggcaaaa ggacgatcat
     4921 cggcttgcgc ccgacgcgat ccgcgagcaa tccgctcaag ccactcagca cagcggacat
     4981 gatggcgacg acgacaaaga ggaagccaag ctccagcgcg ttgaagccga tcagcgccag
     5041 gtaaatcggc acgacaatgc ctgccagcgc gcgagcagcg ctcatcgaga ctcgcgccgc
     5101 caacagcagg cgcatatcgc gggtcagcca ggccggggaa gtaagctccc gtagacgttg
     5161 cctcaaggta agttt
//
LOCUS       DASZAK010000472         2914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_651306, whole genome shotgun sequence.
ACCESSION   DASZAK010000472 DASZAK010000000
VERSION     DASZAK010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651306"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VGT44_08245"
     CDS             <1..390
                     /locus_tag="VGT44_08245"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEV2580827.1"
                     /translation="YEKVSDSTMIPPTHRMKSNMEDIAKGMKIAGAISGATIKAVKTI
                     QDTIQSAQGSQTTPNTPPATQQGAAQQGQPNQASQRPPASSPPQPTYTPPAASPQQDD
                     ETLFCDQCGAPVRPGKNFCSRCGARLP"
     gene            578..2116
                     /locus_tag="VGT44_08250"
     CDS             578..2116
                     /locus_tag="VGT44_08250"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2580828.1"
                     /translation="MSQTFLNGNQATEEASNGDSSIRHVRVAILGTGFSGLGMAIRLK
                     QRGEHDFVVFEKASDIGGTWRDNTYPGCACDIPSHLYSFSFALNPRWSHAYSRQPEIW
                     DYLRRCAKRYGIMPHIQWNCEMQDATWDEDEQRWHITTSQGRFSAALLILGYGPLSEP
                     RLPAIPGIEDFEGVMFHSAQWKHDYDLTGKHVAVIGTGASAIQFVPQIQSKVGHISLF
                     LRTPPWILPRLDHPIRLWKQNMYRLLPFTQRMKRARIYSRLEIAALGLVYRPQGLDAA
                     MNIARQHLEKQVPDPILRAKLIPDYTLGCKRILLSDDFYPSLSQPNVEVISDRIREVR
                     AHSVVAEDGREFEVDTIICGTGFHVTDSQLPYSVYGLGGQSLGERWRDGASAYLGATV
                     SGFPNMFLLIGPNTGLGHNSMVYMIESQITYILDCIGIMDRKHIQAIEVRADAQETFN
                     AELQQRMGNTVWKSGCTSWYLDAHGRNTTLWPGFTFEFRRRTRRFDPQNYLLSAKVQN
                     RIKL"
     gene            complement(2074..>2914)
                     /locus_tag="VGT44_08255"
     CDS             complement(2074..>2914)
                     /locus_tag="VGT44_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV2580829.1"
                     /translation="CAFVTFTPIYGTEAVDAPTHRGWGWALDLMRRVPDAPPGAMDLL
                     LVRAVERFRLAGAYRVSLGLVALADTRQEMASVARRPVNFMANHSGLFEQRQSLFDFK
                     QKFHPCWESRYVVAHRTLALPKIALAVLRLRNYFGKGVVGVMAQCLRYCLVGGANTII
                     DMTILNALLWRFPTHNVQFLVVYNSLAYASGAVSSFFLNKYWTFRREQRPTRREVRRF
                     AISLLLEVIYSCILVWFAGKVLQPLITNVILWGNASKLIAVVVGTIISYSLMRFWTFA
                     DSK"
BASE COUNT          695 a          900 c          764 g          555 t
ORIGIN      
        1 tacgaaaaag tttcagactc gaccatgatt cctcccacgc accgcatgaa aagcaacatg
       61 gaggacatcg ctaaaggcat gaagatcgcg ggcgcgatca gcggagcaac catcaaggcg
      121 gtcaagacga tccaggatac catccaatca gcgcaggggt cacagacaac tcccaatact
      181 ccacccgcta cacagcaagg cgctgctcag cagggtcagc ccaaccaggc atcgcaaagg
      241 ccgcccgcct catctccacc tcaacccacg tatacaccac ctgccgcatc accacaacag
      301 gatgatgaga cactcttctg cgaccagtgt ggcgcgcctg tgcgtcccgg caagaacttc
      361 tgcagccgct gtggcgcaag gctgccgtaa tacacggaac gaattttgta ggagccgatt
      421 gatcggccac gacaggtgca aacggcaacc aaccaggccg ttaaatcggc ggtgagcgcg
      481 atcaatcggc tcctacagtt tagttacttc atcataagat ataatgtgta cattggtcgg
      541 catcgatcaa gcatatgaat ccacgaaggg agcaggcatg tcccagacat tcttaaacgg
      601 caaccaggcg acagaagaag cgtcgaatgg tgactcgtcg atccgtcatg tgcgcgtcgc
      661 tattttgggg acgggtttct ctgggctggg catggcgata cgtctcaagc agcgcggcga
      721 gcatgatttc gtggtgttcg agaaagcctc cgacatcggc ggaacgtggc gcgacaatac
      781 ctatccgggc tgcgcctgcg acatcccatc gcacctgtac tccttctcgt tcgcgctcaa
      841 cccgcgctgg agccatgcct actcccggca accggaaatc tgggattatc tccgtcgctg
      901 cgcgaaacgc tatggcatca tgccccatat tcaatggaac tgcgaaatgc aggatgccac
      961 ctgggatgag gacgaacagc gctggcatat caccacttcg cagggccgct tcagcgcggc
     1021 gttgctgatt ctcggctatg ggccactcag cgaacccaga ctgcccgcca ttccaggcat
     1081 tgaagacttc gagggggtca tgttccactc ggcacaatgg aagcacgact atgacttgac
     1141 aggtaaacat gtcgcggtca tcggcactgg cgcatccgca atccagttcg tcccgcaaat
     1201 tcaatcgaaa gtcggccata tttccttgtt tctgcgcaca ccgccctgga ttctcccacg
     1261 cctcgatcat cccatccgcc tctggaagca aaacatgtat cgtctcctgc cctttacgca
     1321 gcgcatgaaa cgcgcgcgca tctattcgcg gctcgaaatc gccgcgctcg gactggtcta
     1381 ccggccgcaa gggctggatg ccgccatgaa catcgcgcga caacacctgg aaaagcaagt
     1441 gcctgatccg atcctgagag caaagctgat acccgactat accctgggct gcaagcgtat
     1501 cctgctctcc gacgacttct acccatcgtt gtcgcagccg aacgtcgagg ttatcagcga
     1561 ccgcattcgc gaagtgcgcg cgcacagcgt cgtcgccgag gatggcagag agttcgaggt
     1621 tgacaccatt atctgcggca ccggctttca cgtcaccgac tcgcaattgc cctacagcgt
     1681 gtatggcctg ggcgggcaat ccctcggcga gcgctggcgg gacggcgcca gcgcctatct
     1741 cggcgcgacc gtctccggct tccccaacat gtttctgctg atcggcccca ataccggcct
     1801 cggccataat tcgatggtct acatgatcga gtcccagatc acctatatcc ttgactgtat
     1861 cggcatcatg gatcggaaac atatccaggc cattgaagtg cgcgccgatg cccaggagac
     1921 cttcaacgcg gaactccagc agcgtatggg aaacactgtc tggaaatcag gttgcaccag
     1981 ctggtatctc gacgcgcacg gacgcaatac cacactctgg ccaggcttca cctttgaatt
     2041 tcgccgcaga acgcgccgct tcgacccgca gaactatttg ctgtcggcaa aggtccagaa
     2101 tcgcatcaaa ctgtaggaga tgatcgtgcc aacgactacc gcaatgagtt ttgaggcgtt
     2161 accccatagg atgacattgg taatcaaggg ctgcaagacc ttgcctgcga accacaccag
     2221 aatacagctg taaatgacct cgagcagcaa gctgatggca aagcgcctta cttccctgcg
     2281 ggtaggtctt tgctcgcgac gaaacgtcca gtacttgttc aagaaaaaac tacttactgc
     2341 gccactggca taagccagcg agttatagac caccaggaac tgcacgttgt gcgtaggaaa
     2401 gcgccagagc agggcattca gaatagtcat atcaatgatc gtgttcgccc cacccaccag
     2461 gcagtagcgc aggcattggg ccatcactcc cactactccc ttgccaaagt agttgcggag
     2521 acgaagcacc gccagggcaa ttttcggcag agccagcgta cgatgagcga caacgtaccg
     2581 gctctcccag caggggtgaa atttctgttt gaagtcgaaa agcgactggc gctgctcgaa
     2641 cagccctgag tgatttgcca taaaattaac aggccgtcgc gctacagaag ccatttcctg
     2701 cctcgtatca gccagggcca ccaggccgag actgaccctg tacgctccag caagtcgaaa
     2761 gcgctcaacc gcccgcacca gcaacaggtc catcgcgcca ggcggcgcat ccgggacccg
     2821 gcgcatcaga tcgagcgccc agccccagcc tcgatgggtg ggtgcatcga ctgcctcagt
     2881 tccgtagata ggggtaaagg tcacgaacgc acaa
//
LOCUS       DASZAK010000473         4740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_367370, whole genome shotgun sequence.
ACCESSION   DASZAK010000473 DASZAK010000000
VERSION     DASZAK010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367370"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VGT44_08260"
     CDS             complement(<1..625)
                     /locus_tag="VGT44_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEV2580830.1"
                     /translation="MRYFWKIDDGSKIKLKDFDPDAIDKQVDRTSTTAELEKLSSELS
                     ELQELMAAAQHHSMLVILQGMDTSGKDGTIRHVLSHVNPQGCQVVSFKEPTSEELAHD
                     FLWRVHRVTPRLGNMGIFNRSHYEDVLVARVHNLVPEKVWSKRYNEINHFEHLLANNR
                     TIILKFFLHISYEEQERRLLAREQDKQKAWKLSASDWTERRYWNDYQQ"
     gene            879..3266
                     /locus_tag="VGT44_08265"
     CDS             879..3266
                     /locus_tag="VGT44_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEV2580831.1"
                     /translation="MSVVPSDQPSGELSQSGIAEAPDEGAETSEATETIEAVETIQAT
                     EASEATGSIEATEAATPDDISEESKAKEEPAASASPPAPPPGRRRPRSTRRRVVMVAI
                     LLLGVLLPVGVAVGYGVNAYSTYNTLKGQAYDGVQHLLNVKTVFTGLKAHPSGFFDAG
                     KLESAQQQLAAARQDFTELSYEIDHTPIIQTITTYLPQYRAQVQAARAASHIGVDVAQ
                     AGQELVATAMLLAPSFSGQVLADASRPLITQSTLNLVGSTVAELMPQLTDIQQQARLM
                     PLASLPVSSAQRSEIGQLLQALPQAQNDLQEGLNLLGAIGWLLGVDAPRAFLVQTMDR
                     SEIRGTGGFTGQYGELTINGGRVAPFALRDISLVEYTASNKTLGQPAPAQYQSWWPFA
                     NFGLRDSNISADFPTSARLAIAQYQNEVGRSVEGVIVFTPFLIEHVLQIVGPVYIPGY
                     NETITAQNLEDRLHYYQQDNAGIAKQIALQPGNTSTSARKRFTSQLASDLLAKVRQAP
                     PDELIAIASEMLHDLKTKDLQVYFTNPQIEALLAQYGDAAQLDRSTSHDGLYVVQQNL
                     SASKASQYVQTIMKDTVTLDANGGATHLLQMRLVYNQIGPVYGYDTYRDYVRIYVPPT
                     ARLLYGDGFDSGTPLCGGPYAACPANGIYPGGELTCPTGLYQAGAHPPYITAPDGGSA
                     GPLDTIGPPNNLQSDEAGRAMFGGWVILPKNCTMTVTLSWYVPPVGNAPYTLLVQRQA
                     GLFPELDLTILPTPGDCAALHLAPYHFDGLMGEDMSFTPPASKAGADGCYPQPGI"
     gene            complement(3320..4585)
                     /locus_tag="VGT44_08270"
     CDS             complement(3320..4585)
                     /locus_tag="VGT44_08270"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEV2580832.1"
                     /translation="MSQFPRACPSCGSPVPAGQRFCSNCGTDLLSGPAGPSSQYGGQA
                     PQSQQPYAQPQYGQQPYGQYGQPPIQSYQQPQKSNPFAEALGALGLLFFLRRYRPGYR
                     PRRQSSGCCGCLVALIILGLLFGAPAYFYVKQNPKFVQQFQNQIQNSSSNNGSVPTTQ
                     PAVTTTKTGQTVNYVGVDITVVDVKQSTAFIDDNNTATNGMIRLDIKESTGQNVGFYS
                     YGDAFRLILPDGTSVSPVGEETSGSPQTATSRNNWVDFQVSTSIKPDQVQLLLGTPQE
                     AQIKFLLTGKVDPQFLPKTATLNIPISYGGVNWTLKTATSSLSSGGKQASTGMHYVVL
                     TFILDNPTSQTSNTSFTGDYMRLQSGSAKNRPTDSTLPDATANTSALSGTVTFLMPNN
                     GNAFTLILLAIPGGIDPHSNVQVNTDFKI"
BASE COUNT         1015 a         1301 c         1380 g         1044 t
ORIGIN      
        1 cttgctggta atcgttccag taccggcgtt ccgtccagtc gctggctgag agcttccagg
       61 ctttctgttt gtcctgctcg cgggctaaca ggcggcgctc ctgctcctcg tagctgatgt
      121 gcaggaagaa cttgaggata atggtgcgat tattcgccag gagatgttca aagtggttga
      181 tctcgttgta ccgcttgctc caaacttttt ccggcacgag gttgtggacg cgcgcgacca
      241 gcacatcttc gtaatgcgag cggttgaaaa tgcccatgtt tcccagccgg ggtgtgaccc
      301 tgtgaacgcg ccacagaaaa tcgtgcgcaa gttcctcgct ggttggttct ttgaatgaca
      361 cgacctggca gccctgcggg ttgacgtgcg aaagcacatg gcggatggtg ccgtctttgc
      421 cgctcgtatc cataccctgg agaatcacga gcatgctgtg atgctgcgcg gctgccatca
      481 actcttgcaa ctcgctcagt tcgctgctca gtttctctaa ttcggcagtt gtggaagttc
      541 ggtcaacctg tttatcaata gcgtcgggat cgaagtcttt caacttgatc ttgctaccat
      601 catcaatttt ccagaaatag cgcatcgtcg gtctccttgc atagctgcgt ttcggcgtgt
      661 atgctacatg ataacataca tttcgacatt ccttgataaa gggagcggga tgcaggaaat
      721 acttgtgggg caagcgagca tcagaacccg atatttatcc gacggctgtg cgggcattag
      781 ataccatttg ttgagcgtgt gaaacgaact atggccgtct cccgttgtag caagtgatat
      841 agtgaaatcg agaccatatt ttatgccggg aatgcgtcat gtccgtagta ccatccgatc
      901 aaccgagcgg ggaactttct caatcgggaa tagcagaagc gccagacgag ggtgctgaga
      961 catcagaagc gacagagaca attgaagcgg tagagacgat acaggcgaca gaggcgagtg
     1021 aagccacagg ctcaatagag gcgacagagg cggctacccc tgacgatatt agtgaggaat
     1081 ctaaggcgaa agaggagccg gctgcaagcg cgtccccacc agctccccca cctggaagac
     1141 gcagaccgcg ctcaactcgc cgtcgcgtgg tgatggtggc tattttgctg cttggcgtgc
     1201 tgctgcctgt tggtgtggca gtgggctatg gcgtcaatgc gtattcgacg tataacaccc
     1261 tcaagggcca ggcgtatgat ggggtgcagc atttgctgaa cgtcaaaacg gtctttacgg
     1321 gcctgaaagc gcatccgagc ggcttcttcg atgccggcaa gctggagagc gcgcagcagc
     1381 agcttgcagc ggcccgccag gatttcaccg aactttccta tgagatcgac catacgccga
     1441 tcatccagac catcacgacc tatctcccac agtatcgcgc gcaggtacag gctgcgcggg
     1501 cagccagtca tatcggcgtc gatgttgcgc aggcagggca ggaactggtg gcgacggcca
     1561 tgctgctcgc gccctctttt agtggacagg tactggccga cgcgagcaga cccctgataa
     1621 cgcagagtac gctcaacctg gtgggcagca cggttgccga actgatgcca caattgacgg
     1681 acatacagca gcaggcgcgc ctgatgccgc tagcgagcct gccggtcagc agcgcgcagc
     1741 gcagcgaaat tgggcagttg ttgcaggcgc ttccacaggc acagaacgat ttacaggaag
     1801 ggttgaactt gctgggagcg attggctggc tgctcggtgt ggatgcgccg cgcgcgttcc
     1861 tggtacagac gatggatcgc tccgagatac gtggaacagg tggcttcacg gggcaatatg
     1921 gggagttgac catcaacggc gggcgcgtcg cgccgtttgc cctgcgagat atctcgctgg
     1981 tggagtatac cgcttccaac aagacactgg gacaacccgc cccggcgcaa taccaatcgt
     2041 ggtggccgtt cgccaacttt ggcctgcgcg actccaacat ctccgcggat tttcccacgt
     2101 cggcgcgcct ggccatcgcg caataccaga acgaggtcgg gcgttccgtc gaaggtgtga
     2161 tcgtcttcac gcccttcctg atcgagcatg ttttgcagat cgtcggcccg gtctatatcc
     2221 ctggctataa cgagacgatt acggcgcaga atctggagga tcgccttcat tattatcagc
     2281 aggataatgc cggcatcgcg aagcaaatcg ccctgcagcc gggcaatacg tcaacctcgg
     2341 cgcgcaaacg cttcaccagc cagcttgcga gcgatttgct ggcgaaggtg cggcaggcgc
     2401 cgcccgacga gctgatcgct atagcgagcg agatgctgca tgatttgaag acgaaagatt
     2461 tacaggtcta cttcactaac ccacagatcg aggcgctgct ggcgcaatac ggcgacgcgg
     2521 cccagctcga ccgctcgacc agccacgatg ggctgtacgt ggtgcagcaa aacctgagcg
     2581 ccagcaaagc ctcgcagtat gtccagacga ttatgaagga taccgtcacg ctggatgcga
     2641 acggcggcgc gactcacctg ttgcagatgc ggctggtcta caaccagatc gggccggtct
     2701 atggctacga tacttaccgt gattatgtgc gcatctacgt gccgccgacg gcgcggctgc
     2761 tgtatggcga cggcttcgat agcggcaccc ccctttgcgg cggcccctac gcggcgtgtc
     2821 cggcaaacgg catctatccc ggcggagaac tgacctgtcc aacgggatta taccaggcgg
     2881 gcgcgcatcc accctacatc actgctcccg acggcggatc ggcggggccg ctcgatacga
     2941 ttggcccgcc caacaacctg caaagcgacg aggcgggccg cgccatgttc ggcggctggg
     3001 tgatcctccc gaagaactgc acgatgaccg tgacgctctc gtggtacgtc ccgcctgttg
     3061 gaaacgcccc ttacacgctg ctcgtgcagc gccaggccgg cctcttcccc gaactcgacc
     3121 tgaccatcct gccgacgccc ggcgactgcg ccgcgctgca tctcgccccc taccacttcg
     3181 acggcctgat gggcgaggat atgtcgttta ccccgcccgc gtcaaaggct ggggcggatg
     3241 ggtgctatcc ccagccggga atttagcccc atccgctgaa aacatatccc cagggatgtt
     3301 gatctctatc tcacattagc tatatcttaa aatccgtgtt gacctggaca tttgaatgtg
     3361 gatcaatgcc gcctggtatt gccagaagaa tcagagtaaa tgcgttacca ttattcggca
     3421 tcagaaaggt caccgtcccg ctcaaagcag aggtgttcgc cgttgcatcc ggcagtgtag
     3481 agtctgttgg ccggttcttg gcacttccag attgcaggcg catatagtca cctgtaaacg
     3541 aggtgttact ggtctgcgag gtaggattat ccagaatgaa tgtgagaacg acatagtgca
     3601 tacccgtgct agcctgtttg ccgccggaac tcaacgacga ggtcgccgtt ttcagcgtcc
     3661 agtttactcc tccatagctg atgggtatat tgagagtagc tgtcttggga agaaattgag
     3721 gatcaacttt gcccgtcaac aaaaacttga tctgcgcttc ttgcggagta cccaataaca
     3781 attgaacctg gtcgggtttg atgctggttg agacctggaa gtccacccag ttgttgcggg
     3841 aggtggcagt ttgtgggctt ccgctggttt cttccccaac gggtgacaca gaggtaccat
     3901 caggcaaaat caagcgaaaa gcatcgccat acgagtaaaa tccgacattc tgccctgtgc
     3961 tctctttgat atccagtcga atcatcccgt tcgtggccgt gttattatcg tcaatgaagg
     4021 ccgtagattg cttcacatcg acgacggtaa tatcaacacc cacgtagttc accgtctgtc
     4081 cggttttggt ggtcgtgacg gctggctgcg ttgtagggac gctgccattg ttgctgctgc
     4141 tgttctggat ctggttctgg aattgctgga cgaatttggg attttgctta acgtaaaagt
     4201 atgcaggagc gccaaacaag agacccagaa tgattaacgc cacaagacag ccgcagcagc
     4261 cgctgctctg gcggcgagga cggtagccag gccggtagcg gcgtaggaag aacaacaggc
     4321 cgagcgcgcc cagggcttcc gcgaagggat tggacttctg tggctgctgg taggactgaa
     4381 tcggcggctg tccgtattgc ccgtaaggtt gttgcccata ctgcggttgt gcatagggct
     4441 gctggctttg aggcgcttgc cctccgtact ggctggaggg gcctgctggc ccggatagca
     4501 ggtctgtgcc gcaattgcta caaaaccgct gaccggctgg caccggactg ccgcatgatg
     4561 gacaggcgcg tgggaattgt gacatacgct ttcctcctcg gtgattaagc tattcataca
     4621 tatatgacga agaagtaaag gttcaggttt atgaggttat tgtaaaccta catttccgta
     4681 aagtctattg gcaaattcac ctgtacatca tgagcaggct cacaaaagtt cgtagggaca
//
LOCUS       DASZAK010000474         2770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_224833, whole genome shotgun sequence.
ACCESSION   DASZAK010000474 DASZAK010000000
VERSION     DASZAK010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224833"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="VGT44_08275"
     CDS             complement(<1..609)
                     /locus_tag="VGT44_08275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2580833.1"
                     /translation="MNDFSGQYQNRTFGNYRLLKLIGEGGFSEVYLGEHRYLGNEAAI
                     KVLRTRLTEEEFERFRLEAQTIIGMGHPNMVRVLEFGVEDHIPFIVMSYAPHGSLRDL
                     YPDGTRVPLERIVQLVKQIAAALQYAHDHQVIHRDIKPDNLLVGPDGEVLLSDFGIAV
                     AAHNTYSQKTQEAIGTVAYMAPEQLRRKARTASDQYALAVMVY"
     gene            1047..>2770
                     /gene="uvrB"
                     /locus_tag="VGT44_08280"
     CDS             1047..>2770
                     /gene="uvrB"
                     /locus_tag="VGT44_08280"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEV2580834.1"
                     /translation="MAEFKVEAPFEPTGDQPEAITSLVKGLSNGYKKQTLLGVTGSGK
                     TYTVAKIVEQVQKPTLVLAPNKTLAAQLFSEFKEFFPNNAVEYFVSYYDYYQPEAYIA
                     RTDTYVEKESMLNEEIEKLRLSTTRSLFERRDVLIVASVSCIYGLGSPDEYGEVVLTL
                     KVGEERRRERILRHLNSIQYERNDANFIRGRYRVRGDVLEIFPAYEDVAVRIELFGDE
                     IERMTDIDPLTGEVLGKRQRLDIYPAKHWVTTQERLNHALEAIEEELEQRLLVLEAEG
                     KVLEAARLKQRTNFDLEMLRETGFCSGVENYSRHLAGRGAGEQPWTLMDYLPDDYLLV
                     VDESHVALPQVRGMFAGDRSRKQVLVDYGFRLPSALDNRPLTFDEFEKHIRQALFVSA
                     TPGPYENQNSAAIVQQVIRPTGLLDPQISVRPTKGQIDDLLAEIRKRVEKKQRVLVTT
                     LTKKMSEDLADYLQELNIKVHYLHSEIDTIERVEILRDLRLGVYDVVVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSEGSLIQTIGRAARHIDGSVIMYADNRTKSMQRAIDETN
                     RRRELQTD"
BASE COUNT          623 a          771 c          814 g          562 t
ORIGIN      
        1 gtagaccatc acagccagcg cgtactgatc gctggccgtg cgggccttcc tgcgtagttg
       61 ctcaggcgcc atatacgcaa cggtgccaat cgcctcctgc gttttctggg aataggtgtt
      121 atgcgcggcc accgcgatgc caaagtcgct gagcaggact tccccatccg ggccaaccag
      181 gagattatcc ggcttgatat cacgatggat cacctggtga tcgtgggcgt attgcaacgc
      241 tgcggctatc tgcttcacca gttgcacaat gcgctcaagc ggcacgcgcg tgccatcggg
      301 atagagatcg cgcagcgagc catgcggcgc gtaactcatc acaataaatg ggatgtgatc
      361 ctccacacca aattccagca cacgcaccat attgggatgc cccatgccga tgatggtctg
      421 cgcctccagg cggaaacgct cgaactcctc ttccgtcagg cgggtgcgca gcaccttgat
      481 agccgcttca ttgcccaggt aacgatgctc gcccaggtag acttcagaga aaccaccttc
      541 accgataagc tttaagagac gatagttccc aaatgtcctg ttttgatatt gcccagaaaa
      601 gtcattcatg tctgcgcctc gatatgtatg ggggagatgt aatgtacagt tatactacgt
      661 ctccgaggac gaggattccc acgcaggttg attgttttca cagattagct gatacttgtt
      721 tgctggcaaa tgtcgcgatc atccccccat ccctgtcatg ctgagcgcag cgaagcatct
      781 cacagatcat cgaccgagac ccttcgctgc gctcagggtg acaaactcat accgatctcg
      841 ttctgtcatg ctgagcgcag cgaagcatct cacaggccat cgaccgagac ccttcgctgc
      901 gctcagagct tgccctgaac gcagtgaagg gatgacaaca ccgatcctgg ttgggaatgt
      961 gcattgtgcg caaatttcct cataacgtga tataatacag aggcatttgg aaagtatttt
     1021 ctatacatat gtaaggaaca atgacgatgg ccgaattcaa ggtagaggcg cctttcgagc
     1081 cgacgggcga ccagccggag gcgatcactt cgctggttaa ggggctatcc aacggctata
     1141 agaagcagac gctgctgggc gtgacgggta gtggcaagac gtatacggtg gcgaagatcg
     1201 tcgagcaggt gcagaagccc acgctggtgc tggcgccgaa caagacgctg gcggcgcagt
     1261 tgttcagtga attcaaagag tttttcccca ataatgccgt tgagtacttt gtctcttact
     1321 atgactacta tcagccggaa gcgtatattg cccgcacaga tacttatgtc gaaaaagaga
     1381 gtatgctgaa cgaagagatc gagaaactgc ggctctcaac cacgcgcagc ctcttcgagc
     1441 ggcgcgacgt gctgattgtg gcctcggtct cgtgtattta tggcctgggc agcccggatg
     1501 agtatggcga ggtggttctg acgctcaagg tcggcgagga gcggcggcgc gagaggattc
     1561 tgcgacacct gaacagcatc cagtacgagc gcaacgacgc caacttcatt cgcgggcgct
     1621 accgtgtgcg tggtgatgtg ctggagattt tcccggcata cgaggatgtc gcggtacgca
     1681 tcgagttgtt cggcgacgag atcgagcgca tgaccgatat cgacccgctt acaggcgagg
     1741 tgttgggcaa acggcagcgc ctggacatct acccggccaa gcactgggtc acaacgcagg
     1801 aacgcctgaa ccacgccctc gaagccatcg aggaagagtt agagcagcgc ctgctggtac
     1861 tagaggcgga gggcaaggtc ctggaggcgg cgcgcttgaa acagcgcacg aacttcgacc
     1921 tcgagatgct gcgcgagacg ggcttttgtt ctggcgtcga gaactattcg cggcacctgg
     1981 cgggcagagg cgcgggtgag cagccctgga cgctgatgga ctacctgccg gacgactatc
     2041 tgctggtggt ggacgaatcg cacgtggcgc tgccgcaggt gcgcggcatg ttcgcaggcg
     2101 accgctcgcg caagcaggtg ctggtcgact acggcttccg cctgccctct gcgctcgaca
     2161 accgcccgct caccttcgac gaattcgaga aacatatccg acaggcgttg ttcgtatccg
     2221 ccacgcctgg cccatacgaa aaccagaaca gcgccgccat cgtgcagcag gttattcgcc
     2281 cgacgggttt gctcgatccg cagatcagcg tgcgtcccac caaaggccag atcgatgacc
     2341 tgctggcgga gattcgcaag cgcgtcgaga agaagcagcg cgtgctggtg acgacgctca
     2401 ccaagaagat gagtgaagat ctcgcggact acttgcaaga attaaatatc aaggtgcatt
     2461 atctgcattc agaaatcgat acgattgagc gcgtcgaaat cctgcgcgac ctgcggctgg
     2521 gcgtctacga tgttgtggtc ggtatcaacc tgctgcgcga agggttggac ctgccggagg
     2581 tatcgctggt ggccatcctg gacgctgata aggagggctt cctgcgctcc gaaggctcgc
     2641 tgatccagac cataggccgc gcggcgcgtc atatcgacgg cagcgtgatt atgtacgccg
     2701 ataacaggac aaaatcgatg cagcgcgcca tcgatgaaac caaccgccgc cgcgagctgc
     2761 aaacagactt
//
LOCUS       DASZAK010000475         6034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_438436, whole genome shotgun sequence.
ACCESSION   DASZAK010000475 DASZAK010000000
VERSION     DASZAK010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6034
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438436"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /gene="kdpA"
                     /locus_tag="VGT44_08285"
     CDS             complement(<1..1736)
                     /gene="kdpA"
                     /locus_tag="VGT44_08285"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEV2580835.1"
                     /translation="MPVTLNGVLQVAIFLAIVLLLTKPVGLYMYKVFNGERTWLSPVL
                     VPVERFFYRLSGINPEEEQKWTGYMIAVLIFSVVGMLLLYAFERTQQWQGTFFNPQGL
                     PNVEPQLAFNTAASLTTNTTWQNYGGETTMSYLTQMAGLTFHQFVSAAAGIALAVALV
                     RGLARRSAQHLGNFWVDVTRCTLYILLPICIVIALIYVSQGMIQNFNPYTVVHTLNGQ
                     TQTIAQGPVASMEAIKDLGTNGGGFFNTNSAHPFENPNALTAMLIILEEITLGAGLFY
                     MFGKWVGDTRQGWVLWIVSAIIFTIGLAVVIPAEQTGNPLLTAAGANQQVSATQAGGN
                     MEGKEVRFGITVSALQEVTTTATSTGTVMSFHDSYTPLGGLVLITNIALGELIFGGVG
                     SGLYSMLYFAIIAVFIAGLMVGRTPEYLGKKIERKEIMMAVLALLISPLLVLSFTALA
                     MALPAGLTAISNAGPHGLTEVLYAFTSANGNNGSAFAGLSSNTPFYNWTLGMVMLLGR
                     FAEVIPVLAFGGSLVKKRVVPAGLGTFPTTGPLFVALLIGVILIVGALTYFPAYALGP
                     IVEHLLMQAGRT"
     gene            complement(1759..1854)
                     /locus_tag="VGT44_08290"
     CDS             complement(1759..1854)
                     /locus_tag="VGT44_08290"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit F"
                     /protein_id="HEV2580836.1"
                     /translation="MNTPLEYILVGIVTLGILIYLIIALLAPEKF"
     gene            complement(1949..2422)
                     /locus_tag="VGT44_08295"
     CDS             complement(1949..2422)
                     /locus_tag="VGT44_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2580837.1"
                     /translation="MPGARILLVEDDEVLRDLMARNLQVRGHEVQVADNAQSALATLR
                     VTPVDLVLLDINLPDETGWEVLRIAKKHGWLHQQALGGPTQCFPVVVLSAVRINPRRL
                     AEFHPLAYLPKPFPIEAVLRLAAEAADRRNGSHVTEQKHEDDAYSAVVDEEDMYA"
     gene            complement(2448..3023)
                     /locus_tag="VGT44_08300"
     CDS             complement(2448..3023)
                     /locus_tag="VGT44_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2580838.1"
                     /translation="METRRWLLPFTWGMDMQAIDAAVRLAEGSGATLVAVSLISKPDG
                     SRGVRLEHIQQSKDFLAAVELKANQLRVPLNRHEVITVDAIQSITTLTHELRCDAIVL
                     VSRGEREALLRAHQLKRLLVEPPASLLVLRLTRQTGEQLVKNTWLGARIWARLRQRME
                     RGEGQHGPGPEPAPEEPLWIRSEAHRRGMSL"
     gene            3430..5079
                     /locus_tag="VGT44_08305"
     CDS             3430..5079
                     /locus_tag="VGT44_08305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2580839.1"
                     /translation="MSRLLRFLLDTLLAFVGSMLVTAIIYAFQLYPRIPNISIIYLLV
                     VMTLATTRGRYAAILASFVAFLAFDYFLVPPLYVFTINRVEEWIALFVFLVTALLTSQ
                     LAVSLRGRAEQASRRERETRLLYDLVRATNREAEPERQLLANAQAVVDVLSPWGVHDC
                     AILQPDAAGTLQIHASAYQPVEQIARSSDEQAIASWVMAHGRAMGLYDDAALALSTSA
                     RFVQRVIVWSTVAGRAVRRSLRLIPLMVGQKVVGVLRLRVLDDADNFPQEELLEEERS
                     RPGGANSFFWTFLDQVTALVERARLQQDNLRIAILQRTDALRAALLSSVSHDLRTPLT
                     SIKAAASSLLQEDVQWDEDARRGFAQSIEQEADRLNRLVENLLDMSRIEEGVLKPERE
                     EYALVSLIHDVVDRLATLLGGREVRIDLPDDLLLVDIDYLQIDQVLTNLLENAVRYTP
                     QESPIEISARIEGEEVVLSVADRGPGIPPAELERVFDKFYRVLHGQRRLDSSMGSGLG
                     LAVCKGLVEAHGGHIKAAPRDGGGLVMSVTLPRSVQAKEDL"
     gene            5076..5780
                     /locus_tag="VGT44_08310"
     CDS             5076..5780
                     /locus_tag="VGT44_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580840.1"
                     /translation="MSKLGATILVVDDEREIVRALRRSLTAHGYAVLSARSGEEAIEV
                     VSQQRPDLLLLDLLLPGIGGLEVCRQVRASSNMPIIVLSVKDAERDKVEALDLGADDY
                     VAKPFGMNEVLARVRVALRRMAQPQYGAAPRFEAGPLSVDFAQRRVRLDEQEIALTPT
                     EYDLLKVFILHRGKILTRQQLLKEVWGAQSHARTHSLHVYVAQLRQKIESVPEHPRFI
                     HTIPGVGYRFSYEFEG"
BASE COUNT         1196 a         1787 c         1767 g         1284 t
ORIGIN      
        1 aacgtcctcc ccgcctgcat cagtagatgt tcgacgatcg gccccagcgc ataggccggg
       61 aagtaggtca gcgcgcccac gatcaggatc acaccgatca agagcgcaac gaacaacggc
      121 cctgtcgtcg ggaaggttcc caggccagct ggaacgacgc gcttcttgac gagcgagccg
      181 ccgaaggcca gcacgggaat aacctcggca aagcggccga gcagcatgac catgccgagc
      241 gtccagttgt agaatggcgt gttactgcta agacccgcga acgccgatcc gttgttgccg
      301 ttggctgacg tgaaggcgta taacacctcc gtcaacccgt gcggcccggc gttgctgatc
      361 gctgtgagac ccgccgggag cgccatggca agggcggtaa agctcagcac cagcagaggc
      421 gaaatgagga gggccaacac cgccatcatg atctccttgc gttcgatctt cttgcccagg
      481 tactccggcg ttctccccac catcagcccg gcgatgaaga ccgcaatgat ggcgaagtag
      541 agcatactgt agagtcctga gccgacccct ccaaagatga gttcgcccag cgcgatgttg
      601 gtaatcagca ccagcccgcc cagcggcgtg tagctatcgt ggaagctcat gacggtcccc
      661 gtcgatgtgg cggtcgtcgt cacttcctgg agggccgaga cggtgatgcc gaagcgcacc
      721 tccttgccct ccatgttgcc gcccgcctgt gtcgcgctga cctgctggtt cgccccggct
      781 gccgtcagaa ggggattgcc ggtctgttcg gcggggatca cgacggccag tccgattgtg
      841 aaaatgatgg ccgagacgat ccacagtacc cagccctggc gcgtatcgcc cacccatttg
      901 ccgaacatgt agaacagacc cgcccccagg gtgatttctt caaggataat cagcatagcg
      961 gtgagggcgt tggggttctc gaagggatgg gcggagttgg tgttgaagaa gccaccgccg
     1021 ttggttccca ggtccttgat cgcctccatc gaggccaccg gcccctgggc gatcgtctgc
     1081 gtctgtccgt tgagggtatg cacgaccgtg taagggttga agttctggat catgccctgt
     1141 gagacgtaga tcagcgcgat tacgatgcag atcggcagca ggatatacag cgtacagcgc
     1201 gtcacatcca cccagaagtt gcccaggtgc tgcgcgctgc gacgcgccag gccgcgcacc
     1261 agcgccaccg ccagcgcgat accggcggcg gcggagacga actggtggaa ggtcaggccg
     1321 gccatctgcg tcagataact catcgttgtc tcgccgccat agttctgcca ggtggtgtta
     1381 gtggtgaggc tggcggcggt attaaaggcg agctgcggtt cgacattggg cagaccctgc
     1441 gggttgaaga acgttccctg ccactgctgg gtgcgctcga aggcatacag gagcaacatg
     1501 cccaccacgc tgaagatcag cacggcgatc atgtagcccg tccacttctg ctcctcttcc
     1561 gggttgatgc cggagaggcg gtaaaagaag cgctcaacgg gaacgagaac cggcgagagc
     1621 caggtacgct ccccgttgaa gaccttgtac atgtacaggc cgaccggttt ggtcagcagc
     1681 agcacgatgg ccagaaagat cgctacctgc aggacaccgt tgagtgttac aggcataggt
     1741 ttacctccta gaagacaatc aaaatttctc aggcgcgaga agcgcgataa tcaagtagat
     1801 gagtatcccc aatgtgacaa tgcccaccag gatatactcc aacggagtgt tcatgccgtt
     1861 ctttcccttt tgttacaggc gctcgcagcc ttcggtgaag ccgatgaacg ccacaaacgt
     1921 gacaatcgtc accacgataa tcagcaaatc aagcatacat atcctcctcg tcaactacgg
     1981 cgctataggc gtcatcctcg tgtttctgtt cggtgacgtg cgagccgttt ctgcggtcgg
     2041 ctgcctcagc cgcaaggcga agaactgcct ctatcggaaa tggcttgggc aggtaggcca
     2101 gcgggtgaaa ttctgcaagg cgtcgcgggt taatgcgcac cgcagacaag accacgacgg
     2161 ggaaacattg cgtcggcccg ccaagcgctt gctggtgcaa ccagccatgt ttctttgcga
     2221 tacgcaatac ttcccagccc gtctcatcgg gcaggttaat atccagcaac accaggtcaa
     2281 ctggagtaac tcgcagagtg gccagcgcgc tctgcgcatt gtccgcgacc tggacctcgt
     2341 gtccacgtac ctggaggttg cgcgccatca ggtcgcgcag gacctcatcg tcctcaacta
     2401 gtaagatgcg tgctccaggc atacatccca cctccttttc tactcttcta caggctcatg
     2461 cctctacggt gagcttccga ccgaatccag agtggttcct ccggtgccgg ttctggtcct
     2521 gggccatgct gcccttcccc acgttccatg cgttgccgca gcctcgccca gatgcgtgcg
     2581 cccaaccagg tattcttcac aagttgttcc cccgtctgcc gggtcaggcg cagcaccagc
     2641 agcgatgccg gtggctctac gagcaggcgc ttgagctggt gggcgcgcag caaggcttca
     2701 cgctcgcccc ggctcaccag cacaatcgca tcgcagcgca actcgtgcgt cagcgtcgtg
     2761 atgctctgta tcgcgtcaac ggtgatgacc tcgtggcgat tgagcggaac gcgcaactga
     2821 tttgccttca actcgaccgc cgcaaggaag tctttggact gttgtatatg ttccaggcgc
     2881 actccacgac ttccgtcggg tttggagatc agcgagactg ccactagcgt ggctccgctg
     2941 ccctccgcca ggcgcacagc cgcgtcaata gcctgcatgt ccatgcccca ggtaaagggc
     3001 aacagccacc ggcgtgtttc cattgtgtcc tcctgctagt acatcaaggt tcaatcgccc
     3061 ctacagatac ctttaccctg ttcggtcgtt aaaagacatc aggtcgattg aaggttggta
     3121 gacacctaac atagtgtagt ggaaaaatgg taaatgtgcg gtctggatac ctccgctatt
     3181 gttctgggtt ttctctaaat gcctgggagt atcctcaaga aaaactcaat gacgctgcct
     3241 gtatgattgt ttgtgtcttg agcttttcta gattatttcg ccgcaaatcc agtacacatc
     3301 ttgagctttt ttaccctata ctctctttgg atgagggatg ttccctggtg agtgcgagtc
     3361 gcgccgacga ggaaaggtcg taccgctatg cgtattgtag agcctgtttt gccaattaat
     3421 gagaaaaaag tgtctcgctt gctgcgattt ttgctcgata ccctgctggc attcgtcggg
     3481 tccatgctgg tcacagctat catctatgct tttcaactct atccgcgcat tcctaacatt
     3541 tcgatcatct acctgctggt ggtcatgaca ctggctacca cgcgaggtcg ttacgccgcg
     3601 attcttgcct ctttcgtggc ctttctcgcc tttgattact ttctcgtacc ccccttgtac
     3661 gtcttcacca ttaaccgcgt ggaagaatgg atcgcgctgt tcgttttcct ggtgaccgct
     3721 ctgttgacca gtcaactggc ggtctcgctg cgcgggcggg cggaacaggc aagccgccga
     3781 gaacgcgaga cgcgcctgct ctacgacctt gtgcgcgcaa ccaaccgcga ggcagagccg
     3841 gagcgccagc tcctggccaa cgcccaggcg gtggtcgatg tgctgtcacc ctggggtgtg
     3901 catgactgcg ccattctcca gccggatgcc gcgggtacgc tgcagatcca cgccagcgct
     3961 taccagccgg tcgagcaaat cgcgcgctcc tcagatgagc aagcaattgc ttcctgggtc
     4021 atggcgcacg gtcgcgcgat gggtttgtac gacgatgccg cactggcttt gtctacctcg
     4081 gcccgttttg tgcagcgcgt catcgtatgg agtactgtcg cgggccgggc cgtgcggcgc
     4141 tctctgcgcc tcatcccgtt gatggttggt cagaaggttg tgggtgtgct gcgcctgcgc
     4201 gtgcttgacg acgcggataa cttcccccag gaggaactcc tggaagaaga gcggtcgcga
     4261 cccggcgggg cgaactcgtt cttctggacg tttttagacc aggtgactgc gctcgtcgaa
     4321 cgggcgcgcc tacaacagga caacctgcgg atcgcgatcc tgcagcgcac tgacgcgctg
     4381 cgcgccgccc tgctatcttc ggtctcgcac gatctgcgca ccccgctgac ctccattaaa
     4441 gctgccgcga gttcgctttt gcaggaggat gtgcagtggg acgaggatgc ccggcgcggc
     4501 ttcgcccaat cgattgagca agaagctgac cgcctcaatc ggctggtgga gaatctgttg
     4561 gatatgtcgc gtatcgagga gggggtactc aaaccggaac gagaggaata cgcgctcgtc
     4621 tcgctgatcc atgacgtggt tgatcggctc gctacgctgc ttgggggtcg cgaagtgcgc
     4681 atcgatcttc ctgacgactt gctgctggtt gatatcgact acctgcaaat cgaccaggtg
     4741 ctgacgaacc tgctggagaa tgccgtgcgc tacactccgc aggaatcgcc aatcgagata
     4801 agcgcgcgca ttgaggggga agaggtcgtt ttgagcgtgg ctgatcgcgg tcccggcatt
     4861 ccccctgccg agttggagcg cgtctttgac aaattctatc gcgtcctgca tggccagcgc
     4921 cgtctcgatt cttcgatggg atcaggatta gggctggccg tttgcaaagg actggtcgaa
     4981 gcgcatggcg gtcacatcaa agcagcccca cgcgacggtg gcggactggt catgagcgta
     5041 acgctgcctc gcagcgtcca ggcaaaggag gacctatgag caaacttggt gcgacaattc
     5101 tggtggtaga tgacgagcgg gagattgtgc gcgctctacg acgcagcctc accgctcatg
     5161 gctatgcagt tctctcggcc agaagcggcg aggaggcaat cgaggtggtc tctcagcagc
     5221 gaccagacct gcttttgctg gatctcttgc tccccggtat tggtgggtta gaggtatgcc
     5281 gccaggtgcg agcgtcctca aacatgccga tcattgtgct ttcggtcaaa gatgccgagc
     5341 gcgacaaagt cgaggcgctc gacctggggg cagacgacta cgtcgccaag ccgttcggca
     5401 tgaacgaagt gctggcgcgc gtgcgggttg ccctgcgccg tatggctcag ccacagtacg
     5461 gcgccgcgcc gcgctttgag gccgggccgc tctcggtcga tttcgcccag aggcgcgtcc
     5521 gcctcgacga gcaggagatt gccctgactc ccaccgaata cgacctgctc aaggtcttca
     5581 tcctccatcg cggtaagatt ctcacgcgcc aacaactcct caaagaggtg tggggcgcgc
     5641 aaagccacgc ccgtacccat agcctgcatg tctatgtagc ccaactgcgt caaaagatcg
     5701 aatcggttcc tgaacatccg cgattcatcc acaccatccc cggcgtcggc taccgcttta
     5761 gctacgaatt tgaaggatag gaattgcatg tgggaatacc ggctcagacg tttggagatc
     5821 cagcctgtca tcctgagcgc agcgaaggat ctcggtagct ggcaagtcag atccttcgct
     5881 gcgctcagga tgacagacac gatttccaaa tgtcagagcc tgtattccca catgcaattc
     5941 ctatccttca agttcgtagc taaagcggta gccgacgccg gggatggtgt ggatgaatcg
     6001 tgggtgttcg ggaaccgatt cgatcttctg gcgc
//
LOCUS       DASZAK010000476         6450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_11726, whole genome shotgun sequence.
ACCESSION   DASZAK010000476 DASZAK010000000
VERSION     DASZAK010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11726"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VGT44_08315"
     CDS             <1..658
                     /locus_tag="VGT44_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEV2580841.1"
                     /translation="KPSEIRETPDGLEAVDTLLYTEREIERIVRTACEMAQGRRKHVT
                     SVDKANVLSSSRLWRKVATRVADDYPDITLDHLLVDTCSMQLVRNPRGFDVIVTENMF
                     GDILTDEASMLAGSMGMLPSASLGTRRTAHGLLGLYEPIHGSAPDIAGQGKANPVAAI
                     LTAALMLRYSFGLQAEANAIEQAVERVIEAGFRTADLREEGKQVVNTQEMGKLIVDAL
                     "
     gene            complement(660..866)
                     /locus_tag="VGT44_08320"
     CDS             complement(660..866)
                     /locus_tag="VGT44_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580842.1"
                     /translation="MGVERSVTRWHLQRQLILREIASLEERLRHPPPVQQAGQNSQAA
                     LQQQLTAAQEKLRGMGDCPRPMMG"
     gene            complement(875..5284)
                     /locus_tag="VGT44_08325"
     CDS             complement(875..5284)
                     /locus_tag="VGT44_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2580843.1"
                     /translation="MSNTFDKLSVLDSFIEEVNSYLPEIETNLKRLTQSSEDMEALEE
                     TYRRTHTIGGSASMMDFPGLSHVAHGMEDILGDVLENVATLDTPTLEFLERGLQRLYR
                     LVKGIRNGIDDNAVIAEDDADYTHYREEVEMLARGYQNGALGDEQIASTPQIVTTSPE
                     PSLEEMLASFRTPVPDGDELEWPEESLLTTSNADVEQALLPREDDTRPTPSVQVPFEQ
                     NSIDQSVVLPEDVPQSSAEDYVADNDENGDDATFVASSTTDADEVTLASAPPAPVIEA
                     PKEAEDASQLPWSSELMTGWEEVETRAVAPTQEPGSMAQLYAEIQERTRHVEDQATTL
                     KRTLTQLRLAASMIETQRVEFKGFLDGSKDALERMEDWAGRAMGLNLRNSPEQVKRYL
                     PLSVMWVANNKLKKVLDLLKQMASNVELTDEQMREGLEQLRASIEASGVLLAQGNASN
                     PFFANESGWTPWEARVAQDEESLRERVMFERRGDMASLRSEIEARLREELSREYESRP
                     VTRAARAELEQLIRDEVRQEFEAKRRLHESVSGLDGRESLLEIEARLRNEIEIEVRQQ
                     FLDQLTGEALSGGAVPTLSGQSPATQYLPTEQVGLPRQVSIPPVPLVQPAPTLPQPMS
                     DSPLSSAGDFNQEAAEIFRQEAEEHLQTISMYVAQLEHTPDDREPIQGIRRATHTLKG
                     AAGMMGFRAIADLSHISEDLLDSVVEGAIPISPTVLSLILDTAEALDMLVNGRSKAGE
                     SEAALHALRLRYAGLLGEQSIQAKAVEEELAEELEASEAALLEESAIKSVSDGRQEGV
                     TMQQLARSDLSVRVPLTKLDELVNLFGELLVNRSVLEERLQRLVRLVADVGVSSNRLR
                     DVGQKLESRFEAATLPSGRSVQVMPGQGNQNGYVNGKNGAGRVEPAHLADFDELELDR
                     YTEFHQLARGLSEGISDMTTLSNEMDAIIRDCEGVFGRESRLNTTFQDRLMKARLVPL
                     STMIPRLYRTARAVALKQHKEFEFALEGQETEVDRTVIEEIAGPLLHLIRNAVNHAIE
                     TPEMRSQQGKSPAGQIKLSAAYEGNLVVITVRDDGTGIDPMRVRNAALARGLIKPEQN
                     LSEGDIIELIFRPGFSTAEVLSEESGRGVGLDVVRDSVSRLRGTLEVDSTPGQGTAFT
                     MKFPTSLAIQNAMMVNVGDQQYAIPTVMVEAIGRMDTFKPAMFAGRPAILVQNEIYAL
                     AMLSQLLGLPTNSPDEKAPIVLVHTDRHRVALVVDSIRGRMDVVMKNLGPHLRHVHGI
                     AGGTVLGNGRVVLILEMVELLTPRQGASSAAASPAAPRRETPPPAIAATVQASLMRSD
                     GAAVPTGRSTTTVNPPAQKSSAPPGGKHILVVDDSPSVRRVVGNMLKQNGWEVQLARD
                     GVEALEMITRETPAAVLLDIEMPRMDGYELMATVRAQEQYRTLPLVMLTSRAAAKHQQ
                     RAMQLGASGYVVKPYQDDELLNILKSLVYGATV"
     gene            complement(5345..>6450)
                     /locus_tag="VGT44_08330"
     CDS             complement(5345..>6450)
                     /locus_tag="VGT44_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV2580844.1"
                     /translation="EHGRGFAVVADEIRLLAERSTESTKRIATLVKSIQGDTYEAVVA
                     MEDSTQEVVKGSQLADDAGRALNSIYTAVERQAQMIEGIARAANEQAQVSENVAIAMG
                     QISEITQQTNVGTQEASMSVSYLAELADQLRASVSTFRLPERTNDMVEIFPDMAGVPA
                     LPGGDQFYQQDMEMSNDWNAGFSQDFLSLPQPGSTGNSGSYQFAYNGQQEYASQQNYA
                     GAQPFAGQQGFDAQQYGNQSNANGQYFSTGFSPFNGGNGGLDRNSGDLGGFDMPAAFD
                     EPAQFDFGQDAGFGNNSQGYYPVPPTTARFPQQNAGSQPGQSAFPPMPGQSGQGGMGQ
                     VIGPRPRMRNSQQGQPAYSQDQMPYQNGGHLGQ"
BASE COUNT         1278 a         1967 c         1817 g         1388 t
ORIGIN      
        1 caagccgagc gagatacgcg aaacgccgga tgggctggag gcggtcgata cactgttgta
       61 cacggaacgc gagatcgaac gcatcgtgcg cacggcgtgt gagatggcgc aggggcggcg
      121 caagcacgtc acgtcggtcg ataaggccaa cgtcctcagt tcgtcgcggc tgtggcgaaa
      181 agtggccacg cgcgttgccg acgactatcc cgacatcaca ctcgatcacc tgctggtcga
      241 tacctgttcg atgcaactgg ttcgcaatcc acggggcttc gatgtgattg tgacggaaaa
      301 catgtttggg gatatcttga cggatgaagc gtcgatgctg gccggttcga tggggatgct
      361 gccctcggcc agcctgggaa cgcgccgcac tgcacacgga ctcctcggcc tgtacgagcc
      421 gatccacggt tccgcgccgg atatcgccgg gcagggcaaa gccaacccgg tcgcggccat
      481 tcttacggcg gcgctgatgc tgcgctactc ctttggcctg caagcggagg ccaacgcgat
      541 cgagcaggcc gtcgagcgcg tgatcgaagc aggattccgc accgcagacc tgcgcgaaga
      601 gggcaagcag gtggtgaaca cacaggagat gggcaaactg atcgtggatg cgctctaaat
      661 tagcccatca tcggtctggg gcaatcaccc atgccgcgca gcttttcctg cgcggcagtg
      721 agttgttgtt gtaaggccgc ctgactgttt tgaccggcct gctgcaccgg tggaggatgc
      781 cgcagtcgct cctcgagcga ggcgatttcg cggagaatca attgtcgctg taagtgccag
      841 cgggtgactg aacgttcgac gcccatagct tcctctagac agtcgcgccg tagaccaggg
      901 atttgaggat gttcagcagc tcatcatcct ggtagggctt gacgacatag ccacttgctc
      961 caagctgcat ggcgcgctgc tggtgcttgg ctgccgcgcg agaggtcagc ataacgagcg
     1021 gcagggtgcg atattgctcc tgggcgcgca cggtggccat caactcgtag ccatccatgc
     1081 gcggcatctc aatgtcgagt agcacagccg ccggtgtttc gcgtgtaatc atttccaacg
     1141 cctccacacc gtcgcgggca agctgcacct cccagccgtt ctgtttcagc atgttcccga
     1201 cgacgcggcg cacacttgga ctgtcatcca ccaccaggat atgtttccct cccggaggag
     1261 ccgacgactt ctgcgcgggc ggattgacgg tcgtcgtgct gcgcccggtc ggaacagccg
     1321 ctccatcgga gcgcatcaaa gacgcctgga ctgtggcagc aattgcggga ggcggcgtct
     1381 cgcggcgcgg cgcggcggga gatgccgcag cgctcgacgc gccctgtcgc ggcgtgagta
     1441 gctcgaccat ttcgagaatc agcacgaccc gaccgttacc caggaccgta ccgcctgcga
     1501 taccatgcac atgccgcagg tgcggtccca gattcttcat aaccacatcc atgcgacctc
     1561 tgatgctatc taccaccagc gccacgcggt gtcgatccgt atgcaccagc acaatgggcg
     1621 ctttctcgtc gggagagttc gtcggcagtc ccagcaactg cgagagcatc gccagcgcgt
     1681 agatctcatt ctgcaccagg atagcgggac gaccggcaaa catagccggt ttgaaggtat
     1741 ccatgcggcc aatggcctcg accatgacgg tcggaatagc gtattgctgg tctccgacat
     1801 tcaccatcat ggcattctga atcgccaggc tggtcgggaa cttcatggta aaggcagtcc
     1861 cctgaccggg tgtcgaatcg acctccagcg tgccgcgcaa gcgtgagacg ctatcgcgca
     1921 cgacatccag gccgacgccg cgaccgctct cttcgctcaa cacctcggca gtcgagaaac
     1981 cagggcggaa aatcaactca atgatgtcgc cttccgacag gttttgctcc ggcttgatta
     2041 gcccgcgcgc cagcgctgca ttgcgcaccc tcatcgggtc gatgcctgtg ccatcgtcgc
     2101 gcacagtaat caccaccagg ttgccttcat acgctgccga gagtttgatc tgtccggcgg
     2161 gggatttgcc ctgctgcgat cgcatctcag gcgtttcgat ggcgtgattg acggcgttac
     2221 ggatcaggtg cagcagcggc ccggcgattt cctcaatcac cgtgcggtca acctcggttt
     2281 cctggccttc cagcgcgaac tcaaactctt tatgctgctt gagcgcaacc gcacgggcgg
     2341 tacgatagag gcgcgggatc atggttgaga gaggcacaag gcgagctttc atcaggcgat
     2401 cctggaaggt cgtattcaag cggctttcac gtccgaagac accttcgcaa tcgcggataa
     2461 tcgcatccat ctcattgctc agggtcgtca tatccgagat accttcgctc aatccgcgcg
     2521 ccaactggtg gaattcggtg tagcggtcaa gttccagttc gtcaaaatcg gccaggtgag
     2581 caggctctac gcgacccgcg ccatttttgc cattgacgta gccgttttga ttgccctggc
     2641 caggcatcac ctgcacagaa cgaccgctcg gcagggtagc agcctcgaag cggctctcca
     2701 gcttctggcc aacatcgcgc agccgattgc tgctgacacc cacgtcggct accagcctga
     2761 ccaggcgctg caagcgttct tccaggacac tacgattgac tagcaattca ccgaagaggt
     2821 tgaccagctc gtcaagcttg gtgagcggaa cgcgcacgct cagatcgctg cgcgcgagct
     2881 gctgcatcgt aacgccttcc tgccgcccgt cgctgacact cttgatagct gactcttcca
     2941 ggagagctgc ctcacttgct tcaagctctt cggccagttc ttcttctacc gccttagcct
     3001 gtatcgactg ttcgcccaac agtccggcgt agcgcaaacg gagcgcatgt aaggcggctt
     3061 cactctcgcc agccttgctc ctgccgttca ccagcatatc cagcgcttcc gccgtatcca
     3121 gaatgaggct caagaccgta ggcgaaatag gaattgctcc ctctaccacg ctgtccagca
     3181 gatcttccga gatatggctc aggtcggcga tggcacggaa gcccatcata ccagcagcac
     3241 ccttgagggt atgggtagcg cgacgaatgc cctggatggg ttcgcgatca tcgggcgtat
     3301 gttcgagctg ggctacgtac atgctgatgg tctggagatg ctcttcggct tcctggcgga
     3361 agatctcggc tgcttcctgg ttaaagtcgc cagccgacga taacgggctg tcggacattg
     3421 gctgcggcag cgtaggagcc ggctgaacga gcggcacagg aggaatggaa acctgcctgg
     3481 gcaaaccaac ctgctcggtc ggcaagtatt gcgtagcggg cgactgtccg gacagggtag
     3541 gcacggcccc cccgcttaat gcttctccgg tcaattgatc gaggaattgc tgacggactt
     3601 cgatctcgat ttcattacgc agtcgggctt cgatctccag aagcgattcg cgcccgtcaa
     3661 gtccgctgac gctctcgtgc aagcgtcgct ttgcctcaaa ttcctggcga acctcgtcgc
     3721 ggatgagttg ttcaagctca gcgcgcgcag cgcgtgtgac cggtcggctt tcgtactccc
     3781 tgctcaactc ctcacgcagc cgagcctcga tctcggaacg cagcgaggcc atgtccccac
     3841 gccgctcaaa catcactcgc tcgcgcagcg attcttcatc ctgggcgacg cgcgcctccc
     3901 agggcgtcca gcccgactcg ttggcaaaga atgggttgga ggcattgccc tgcgctaata
     3961 gaacgccgct ggcctcaatc gaggcccgca actgctccag gccctcgcgc atctgttcat
     4021 cggtcaattc gacgttgctg gccatctgct tcagcaaatc cagcaccttc ttcaatttat
     4081 tgttggctac ccacatgacc gagagtggga ggtagcgttt gacctgctca gggctgttac
     4141 gcagattgag tcccatggcc ctgcccgccc agtcctccat gcgttccagc gcgtccttcg
     4201 agccatccag gaagcctttg aattccacgc gctgcgtctc aatcatcgac gcagccaggc
     4261 gcaattgcgt cagggtacgc ttgagtgtag tcgcctggtc ctcaacgtgc cgagtgcgtt
     4321 cctgtatctc ggcatagagc tgcgccatcg aaccaggttc ctgagtcggg gcgaccgcgc
     4381 gcgtttctac ctcttcccac ccggtcatca gctcgctgct ccacggcaac tggctagcat
     4441 cctctgcctc ttttggcgcc tcgataactg gcgcaggagg agcggaggcc agtgtcactt
     4501 catcggcgtc cgtggtgctg ctagctacaa acgtggcatc atcgccattc tcgtcgttgt
     4561 cagcaacgta atcttctgcg ctagattggg gaacatcctc aggcaaaacg accgactgat
     4621 cgatggagtt ctgctcaaaa ggcacctgta ccgacggggt aggtcgggta tcgtcctccc
     4681 gaggtaaaag cgcctgctca acatctgcat tggaggtggt tagcagcgat tcttcgggcc
     4741 attcaagctc atcgccatcg ggaacaggag tgcggaacga cgcaagcatt tcttccagtg
     4801 agggttcagg tgacgtggta acaatttgag gcgttgaggc aatctgctca tcacccagcg
     4861 cgccattctg atagccccgg gccagcatct cgacctcttc acgatagtgg gtataatcgg
     4921 catcgtcttc tgcaatgacg gcgttgtcat cgatgccgtt gcgaataccc ttgaccaggc
     4981 gatacaggcg ctgcaagccg cgttctagaa actccagcgt gggagtatcc agcgtggcga
     5041 cgttttccag gacatctccc agaatatcct ccatcccatg cgctacatgc gacagtcctg
     5101 gaaaatccat catggaggcg gaaccaccta tggtgtgtgt gcgccgatac gtctcctcta
     5161 gagcctccat gtcttcagat gattgcgtca atcgcttcag gttcgtttcg atttcgggca
     5221 aataagaatt gacctcttca ataaacgaat cgaggactga gagtttatca aatgtattcg
     5281 acatgcttcg cgcacccact cattctagct tcacctcgat ggtctcgaca tgaaacttcg
     5341 attgctactg gcctagatgg ccgccatttt gatacggcat ctgatcctga ctatacgctg
     5401 gctgaccctg ttgtgaattt ctcatgcgtg gacgcgggcc gatcacctgt cccatccctc
     5461 cctgccccga ttggcccggc ataggcggaa acgctgactg accgggctga gagccagcgt
     5521 tttgctgcgg gaagcgcgca gtggttggcg gaacaggata atacccctgg ctgttattgc
     5581 caaacccggc atcctgtcca aaatcaaatt gcgcaggctc atcaaacgca gcaggcatat
     5641 caaacccgcc aagatcgccc gaattccggt ctaatccgcc gttaccacca ttaaatggcg
     5701 agaatcccgt cgagaaatat tgcccattcg cattgctctg gttcccatat tgctgagcat
     5761 caaagccttg ctgcccggca aacggttgtg cgcccgcata gttttgctga gaggcatact
     5821 cttgctgtcc attataggcg aattggtatg agccggaatt gcccgtactc cccggttggg
     5881 gcaacgagag gaaatcctgg gagaaaccgg cattccagtc gttgctcatc tccatatctt
     5941 gctgataaaa ctgatcgcca cccggcaagg ctggcacacc tgccatatcg gggaagattt
     6001 cgaccatatc atttgtgcgc tccggcaggc ggaaggtcga gaccgaagcg cgcaattggt
     6061 cggccagttc ggccaggtaa ctcacgctca tcgatgcttc ctgtgtgcca acgttcgttt
     6121 gctgggtaat ctccgaaatt tgtcccattg caatcgcgac gttttctgaa acctgggcct
     6181 gctcgttcgc agcgcgcgca atgccttcga tcatttgcgc ctggcgctca accgcggtgt
     6241 agatcgagtt caaggcgcgc ccggcatcat cagcgagctg agagcctttc acaacttctt
     6301 gcgtactatc ttccatcgcg acgacggcct cataggtatc gccttgaata ctcttcacca
     6361 gcgtcgcaat gcgcttggtg gattccgtcg aacgctctgc cagcaggcgg atttcgtccg
     6421 cgacaacggc aaatccgcgt ccatgttcac
//
LOCUS       DASZAK010000477         1511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_580109, whole genome shotgun sequence.
ACCESSION   DASZAK010000477 DASZAK010000000
VERSION     DASZAK010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1511
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580109"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..906
                     /locus_tag="VGT44_08335"
     CDS             148..906
                     /locus_tag="VGT44_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580845.1"
                     /translation="MPARTPGNEIPDYPSESLHLEQQYEQTHVGVIGNERLTALVSLV
                     LLVLIIVELVTSAFLRLWLPAHTVVGVLLAGPLLVKVGSTGWRFLRYYTRAPAYVRRG
                     PPPLVLRVLGPVLLVTTLVMVGSGIGLVVTGPLQPFLLMHVFSALVWLPLMALHSLAH
                     LRQVPRNIASDWSSRLGSRSSRGRGLRLAANLGALLLGVIAAVLLFPAATSLFAWSQL
                     HVIGVGPFLVGMGGALLVTLFTRPWRWGRLAEGV"
     gene            1300..>1511
                     /locus_tag="VGT44_08340"
     CDS             1300..>1511
                     /locus_tag="VGT44_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580846.1"
                     /translation="MDHEQGDQETRLDTKKKSIGATERNEEERAAWRENASKLPTESL
                     VFLDECGSNIALTPLYARAPKAERARG"
BASE COUNT          261 a          440 c          484 g          326 t
ORIGIN      
        1 tttcccactg acgttggtcc cactcccccc cagggctacg ccgctgcctt gctggtcgcg
       61 gctttgtgga tgacggtctt tgtcgtctta agtttcgtgg cctttcgacg acaggatatc
      121 accgcgtaac agaggggagg aagcgtcatg cctgcacgca cgcctggaaa tgagattcct
      181 gattatccat ccgagtcact acatctggag caacagtatg agcaaacgca cgtgggagtg
      241 atcggcaatg agcggttgac ggctctggtc agtctcgtgc tcctggtgct gatcatcgtg
      301 gagctggtga cgtcggcgtt cttacgcctc tggctccccg cccatacagt tgtcggcgtc
      361 ctgcttgctg gtccactcct ggtgaaggtg ggttcaacgg gctggcgctt cctgcgctac
      421 tacaccagag cacctgccta cgtccgccgg ggaccgcctc ccctggtctt acgcgtgctc
      481 ggcccggtgc tgctcgtcac caccctggtg atggttggca gtgggattgg ccttgtcgtg
      541 actggcccgc ttcagccgtt cctgctgatg cacgtgttca gtgcactggt ctggctgccg
      601 ctgatggctc tccatagcct cgcgcacctc cggcaggttc ctcggaacat agcctctgac
      661 tggagcagca ggctcggttc ccgctcaagt cgcggacgtg ggctgcggct tgcggccaac
      721 ctcggagcac tgctgctcgg cgtcatcgcg gcggtgcttc tgttccccgc ggcgacctcg
      781 ctgttcgcgt ggagtcagtt gcacgtgatt ggcgtgggcc cgtttctcgt gggcatgggt
      841 ggtgctctgc tggtgaccct cttcaccaga ccgtggagat gggggaggtt ggctgagggg
      901 gtgtaggggc tctcccggcc cgcgccaagc cgacctaaag gagcgcggac tcggcgcgca
      961 atttctgtct actcgttcct gctcatccct tttcaggagc cagctgatga aagcgtactc
     1021 gcaagatctc cgtgaacgtg tgctgcgccc tcgggacctg gggcacccga gggctgagat
     1081 tgtccagctt ttcggtatct ctttggccac actcacgcgg tatgtgaagc aacgacgaga
     1141 tgaagggcat gtgcggccca aagcgagtcc tggtcgtcct ccgaaaaaac gggcacaggt
     1201 ggaggggggc gtgcttctcc aattgcaggc gcatgacgat gccaccctgg aacgacactg
     1261 tgccctgtgg gagcagacgt agggtgaacg tgtgagccga tggaccatga gcagggcgat
     1321 caagagactc ggctggacac gaaaaaaaag tcgatcggag cgacagagcg caacgaagag
     1381 gagcgagccg cttggcgaga gaatgcaagc aagcttccga cagaatcgtt ggtatttctt
     1441 gatgaatgcg gctcgaacat tgccttaacg cccctgtacg ctcgtgcgcc caaggcagag
     1501 agggctagag g
//
LOCUS       DASZAK010000478         2721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_438474, whole genome shotgun sequence.
ACCESSION   DASZAK010000478 DASZAK010000000
VERSION     DASZAK010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438474"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..1033)
                     /gene="otsB"
                     /locus_tag="VGT44_08345"
     CDS             complement(227..1033)
                     /gene="otsB"
                     /locus_tag="VGT44_08345"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEV2580847.1"
                     /translation="MRQQTKIGIESILLQRPLGLAFDIDGTLSPMATTPGEARLYPGV
                     TELLKQARDRAGVHVAIMTGRGIEDGAAIVNIEGLTYSGTHGLEWSDGLPWVHPVQIK
                     PDALAYREAGISLLNLVDKHLSELPGVVVQRKRVGGSIHFRLAPHPDETRLALFALLY
                     EQARKLGMKLREGKRIIEILSPLAIDKGTALRRFADQFDLRGIVFAGDDRTDLDAVRE
                     IALLQREGRAALSIVVRQDETLPDMLNNADILVDGVPGMVALLRKMVGML"
     gene            1527..1904
                     /locus_tag="VGT44_08350"
     CDS             1527..1904
                     /locus_tag="VGT44_08350"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEV2580848.1"
                     /translation="MTMTAGRHFRLLLRRGLRQRCPVCGRGKIFQGFFKTYERCPVCG
                     FEFEREPGYYSGAMAINLVVSELLIAIIAVPLAASQVVPISTLIILGVTLPVLLPILF
                     YRPTKSLWMSFDHFIHPVGSDSI"
     gene            complement(1919..2524)
                     /locus_tag="VGT44_08355"
     CDS             complement(1919..2524)
                     /locus_tag="VGT44_08355"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEV2580849.1"
                     /translation="MSVAHSEQLEAGEHGQGEGRSMNWWGMIFFITSEALIFANFIAA
                     YLYLEIRNGSWQLPTELTYPLINTAILLGSSIPVRIAGAGILKGNMRNLKIGLSLTIL
                     MGAAFLGGQVYEYSGLFGKNFTPQATIFGSAFFMLTGFHGLHVTVGLIFLIIVLLRTL
                     RGDFTAKQHFAVQAGEMYWHFVDGVWIFVFSLVYLLPLIRG"
BASE COUNT          639 a          777 c          702 g          603 t
ORIGIN      
        1 agcagggcca ctcccaccgc gcctataccc aggccaacca gcgagagccg gcggcggatg
       61 cgcgcatact gcttcgcttt gacctgccgc gtttcgtcaa ttgcgacagt ctgcgctggt
      121 cgcgcctcga tgtccataca gtgcgtttct ttctttgcta tctatgcgtg tcatgtacgt
      181 ataaacatat cctacatcac ctatagagca aagggcaaat gcaatcttat agcatcccca
      241 ccatcttccg cagcagcgcc accatgcctg gaacgccatc gaccaggata tccgcattgt
      301 tcaacatgtc cggcagcgtc tcgtcctggc gcacaacgat ggacagcgcc gcccggcctt
      361 cgcgttgcag gagcgcgatt tcccgcacag cgtccagatc agtacggtca tcgcctgcga
      421 agacaatgcc gcgcaagtca aattgatcgg cgaatcgccg taacgccgtg cctttgtcaa
      481 tggcaagtgg agagagaatc tcaatgatgc gcttgccctc gcgcagtttc atcccaagct
      541 tgcgcgcctg ttcgtacagc aaggcaaaaa gcgcgaggcg cgtttcatcg ggatgaggcg
      601 caaggcgaaa atggattgag ccgccgaccc gtttgcgctg gacgaccacg ccaggcagct
      661 cggacaaatg cttatcaacc aggttgagca aggaaatgcc agcctcccgg taagccagcg
      721 catcgggctt gatttgtacg ggatgtaccc acggcagacc atccgaccat tccagtccat
      781 gtgtgccgct ataggtcagg ccctcgatat tgacgatggc cgcgccatcc tcgatacccc
      841 gaccggtcat aatggcgaca tgcaccccgg cgcgatccct cgcttgcttc agtagctctg
      901 tcacgcccgg atacagccgc gcctcgcccg gcgtggtcgc cattggactg agcgtgccat
      961 caatatcgaa tgcgagaccc aggggacgct gtaaaagtat gctttctatt ccgatttttg
     1021 tctgttgtcg cattaattgg agaatcctct atggttttca tagttatatt cgccggatgc
     1081 gcatggttac tcatcccgcg tctgccagct tcatatgctg gttgttcatc tctcatggaa
     1141 gacacctgat aggctctacg gcccctgtca tcaaagcatg tgatgtttct cgaggaatgt
     1201 gagaggcatc attgtgtttg ctcactaaag tagtctccac atctagttta gcacatgaga
     1261 catacttctg tccagttgtg ctaaaaggat gagtgcagaa atagttgtct gcaaccccct
     1321 gtaggagccg atttatcgcg ctcaccgccg attgatcggc gcgaatccac gttgttccga
     1381 agtactggac aggccgatca atcggcggtg agcgcgataa atcggctcct acagcaagca
     1441 ggccatttac gtcaaaagga gaagaggaat gcgcctggta catccagttg ttatacaata
     1501 cagaggaaac actatgaggt gcaagtatga caatgacagc aggtagacat ttcaggttat
     1561 tgctgcgcag aggacttcgg cagcgttgtc ccgtctgcgg gcgaggcaag atttttcaag
     1621 gctttttcaa aacctatgag cgctgtccag tctgtggatt tgagttcgag cgcgaacctg
     1681 gctattatag cggcgctatg gcgatcaacc tggtggtgag cgagctgctt atcgccatca
     1741 tcgccgttcc cctggccgcc agccaggtcg tccccatttc aacgctcatc atcctgggtg
     1801 tgacactgcc cgttttgctg cccatcctgt tctaccgtcc cacgaagagc ctgtggatga
     1861 gcttcgatca ctttattcac ccggtaggca gcgacagcat ataatcgact cctatgcact
     1921 accctcgaat cagcggtagc aggtagacca ggctgaatac gaagatccac acaccgtcaa
     1981 cgaagtgcca gtacatctct ccggcctgca ccgcgaagtg ctgtttggcg gtgaagtcgc
     2041 cgcgcagggt gcgcagcaac acaataatca agaagatcag gccaacggtg acatgcaggc
     2101 cgtgaaagcc ggtcagcatg aagaaagccg aaccgaagat cgtcgcctgc ggcgtaaagt
     2161 tcttgccgaa caggcccgaa tactcgtaca cctggccacc caggaaagcc gcacccatca
     2221 ggatcgtcag tgacaggcca atctttagat tgcgcatgtt gcccttgagt attcccgcgc
     2281 ctgcaatacg gacagggata ctactaccca gcaagatcgc ggtattgatt aatggatagg
     2341 ttagctcggt gggcagttgc cagcttccat tgcgaatctc caggtagagg taagccgcga
     2401 taaagttggc gaagatcaag gcttcggagg tgatgaagaa gatcatgccc caccagttca
     2461 tgcttcgccc ctcgccttgc ccgtgttccc ccgcttcgag ttgttcgcta tgagcaacac
     2521 tcatgataat ttctccctat ggtcaaacga taacatcaat gacgttccag accccaaccg
     2581 atgatggagc cgatcaccag cacaattccg ataataagga gaatgttgga gaacagcaca
     2641 atccccacca gcgaaacaga gatggagacc gccagcaaga aaggccagta gctcttcttg
     2701 gcagacgcca tttgctccat c
//
LOCUS       DASZAK010000479         5594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_117717, whole genome shotgun sequence.
ACCESSION   DASZAK010000479 DASZAK010000000
VERSION     DASZAK010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117717"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VGT44_08360"
     CDS             <1..674
                     /locus_tag="VGT44_08360"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV2580850.1"
                     /translation="DICVLDPDGDLVDHLRAAGRLTHQYNWACYHTRLDTFTHESITY
                     GILGRVVGAPFAVLVAEELFASGCRLLISITSAGQIRSLGQPPYVILVDKALRDEGTS
                     YHYVPPARYSYLHPALREIVWTAWDHGQVPLHLGATWTTDAPFRETEPMIACCREDGI
                     LAVEMEAAALYALASAREFEIVCFAHVSNQMGQNEGDFEKGQAGGSETALRVITQTAR
                     AWMGL"
     gene            complement(669..1478)
                     /locus_tag="VGT44_08365"
     CDS             complement(669..1478)
                     /locus_tag="VGT44_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2580851.1"
                     /translation="MIRFDVNISILLKEVPFLERFAYAARLGFGAVEFWWPGDENLEG
                     VVEQLRQTGLKVALMNFDAGNMGGGDRGFLNDASRKAWFRDHAPRALEFAARIGCQRL
                     NALAGNTIAGERREAQLEMVRENLAYAADRAAAFGLGVVVESLNSFENTRYLLTNTRE
                     TLALLDEVNRPNLQYQYDLYHMQRMEGNLIATLQAHIGRIGHIQVADSPARHEPGTGE
                     IHYPHVLAALDALDYPGYVGLEYNPSSSSEASFGWLPADKRGNISIEDLRL"
     gene            complement(1632..2045)
                     /locus_tag="VGT44_08370"
     CDS             complement(1632..2045)
                     /locus_tag="VGT44_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HEV2580852.1"
                     /translation="MTLLRITAGPFVFTARMEEQDAPQTCAAFSKLLPFRNKIIHARW
                     SGEACWIPLGDFDLNVGYENHTSHPAPGQILIYPGGMSETEILFPYGSARFASKVGQL
                     AGNHYLTIVSGNEYLKELGRKTLWEGAQDIVFEAM"
     gene            complement(2057..3412)
                     /locus_tag="VGT44_08375"
     CDS             complement(2057..3412)
                     /locus_tag="VGT44_08375"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:NF004839.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HEV2580853.1"
                     /translation="MSLYDVLVRNGTAVTTHGTIQADIAIEGGRLVAIAPGLDRDARE
                     EIDATGLHIFPGGIDAHVHFNEPGRAEWEGIATGTAALAAGGATCFFDMPLNAHPPTL
                     DAASFDLKLQAVQASALVDFGLWGGLVPGNLDRLDELAERGVVGFKAFMSNSGIDDFQ
                     AVDDLTLYEGMERAAKLGRIVAVHAENDTLTGALARRAQDEGRTRTRDYLASRPIVAE
                     LEAIERAILFAAETGCALHIVHVSSGRGVTLIAEARAHGVDVTCETCPHYLTLTEEDV
                     VRLGAVAKCAPPIRSQQEQDALWQHIFDGTLPMVVSDHSPAPASMKQDANFFRIWGGI
                     SGCQSLLQLLLTDGYEQRQLPLSLVASLTSANIAQRFSISTRKGQFAPGADADLALVD
                     LRQHEVLEADKLFYRHHHSPYVGKTLRGRIVRTMVRGQTVYRDGKIVSEPIGQLLKPA
                     L"
     gene            complement(3431..4675)
                     /locus_tag="VGT44_08380"
     CDS             complement(3431..4675)
                     /locus_tag="VGT44_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HEV2580854.1"
                     /translation="MSSEAAMARTVMEWCEVLGAISEEPDCLSRPFATQAMRRANATV
                     SAWMRDAGMQVEQDAIGNLIGRYEGSSTLAKTLVLGSHLDSVRDAGKYDGPLGVMIAL
                     ACVQRLHSRGERLPFAIEVVGFADEEGMRFARSYLGSRAITGMFESTWLEAKDAQEMT
                     MAEAIRDFGGNPDPLALRTPRWKREALLGYAEVHIEQGPVLEEANLPLGVVTAIAGQT
                     RLRVGFTGMAGHAGTVPMPMRRDALSAAAEFALGVEAIARGLPGLVATIGQAQVQPGV
                     SNVIPGQVMLSLDVRHQHDTIREQALIEMEARGRIIGEARHITFTWQALETQKAVPCA
                     PYLANLFAQAIEASGYPAHRLPSGAGHDAAIMSALTDMAMLFVRCKGGISHNPAESVT
                     SEDVGLAIDTLERFLLLLAGTF"
     gene            5195..>5594
                     /locus_tag="VGT44_08385"
     CDS             5195..>5594
                     /locus_tag="VGT44_08385"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2580855.1"
                     /translation="MNGVQSVERALDLMECLGRASGWLGISELSSATGQPVGTIHRLL
                     KTLVAREYVVRDSRTRRYAIGPAFRMMVGVGMQGPDWAKIGTPILRELAYLSGETANL
                     AVIDRDRAVYVAQAQPARMVRMFTEIGNRVH"
BASE COUNT         1088 a         1714 c         1660 g         1132 t
ORIGIN      
        1 cagacatctg tgtgcttgat cctgatggcg acctggtcga tcacctgcgc gctgctggtc
       61 gcctcacgca tcagtacaac tgggcctgtt accatactcg gctggatacc ttcacacacg
      121 agagcattac ctatggcatt ctcggcagag tggtaggcgc tccatttgct gtgctggtgg
      181 ctgaggagtt gttcgcctcc ggctgccggc tgctcatcag catcacctct gccggccaga
      241 tacgttcgct gggccagccc ccttatgtga tactggttga caaggctcta cgcgacgagg
      301 gaaccagtta ccattatgtg ccacccgcca gatatagcta tttgcaccct gccttgcgcg
      361 agatagtctg gacggcgtgg gatcacgggc aggttccgct gcacctgggg gcgacctgga
      421 ccacggatgc accattcagg gagacggagc cgatgattgc gtgttgccgc gaggacggca
      481 tcctggcggt agaaatggaa gccgccgctc tgtatgcgct ggcatcggcg cgcgagttcg
      541 agatcgtctg ctttgcgcat gtctcaaacc agatgggcca gaacgagggc gatttcgaga
      601 aaggccaggc cgggggcagc gagacggccc tgcgcgtcat aactcagacg gcgcgcgcct
      661 ggatgggctt atagacgcag gtcttctatg gatatattcc cacgcttatc cgcaggcagc
      721 cagccaaagc tcgcctccga gctgctgctc gggttgtatt ccaggccgac atagccgggg
      781 tagtcgagtg cgtcgagcgc ggccagcacg tggggatagt ggatctcgcc cgtgccgggt
      841 tcgtggcgcg ccggggaatc tgcaacctgg atgtgtccga tgcgcccgat gtgcgcctgc
      901 aaggtcgcga tcaggttgcc ttccatgcgc tgcatatggt agagatcgta ctggtattgg
      961 aggtttgggc ggttgacctc gtcgagtagc gccagcgtct cgcgcgtgtt ggtgagcagg
     1021 tagcgcgtat tctcgaagct gttcaacgac tcaacaacca cacccaggcc gaatgctgcg
     1081 gcgcggtcgg cggcataagc caggttctcg cgcaccatct caagctgcgc ctcgcggcgt
     1141 tcgcccgcaa tggtgttgcc ggcgagggcg ttgaggcgct ggcagccgat gcgcgccgcg
     1201 aactccaggg cgcgcggcgc gtggtcgcga aaccaggctt tgcggctggc gtcgttgaga
     1261 aatccgcggt cgccgccgcc catattgccg gcatcgaagt tcatgagcgc caccttaaga
     1321 cccgtctgtc gcaactgctc cacgacaccc tcaaggtttt catcgccggg ccaccagaac
     1381 tcgaccgcgc caaaccccag tcgggcggcg taggcgaacc gttccaggaa tggcacttct
     1441 ttgagcaaaa tcgaaatatt tacgtcgaat cggatcacga tctacccctt tcgtaaatat
     1501 tgaattttca taaacaagat cggtaatacg ttgtcatcct gagcgcagcg aaggatctcg
     1561 gtctccggcg agtcagatcc ttcgctgcgc tcaggatgac aaaaccggct ttggtcgtga
     1621 aacttcatta tttacatcgc ctcaaatacg atatcttgcg cgccttccca gagcgttttg
     1681 cgccccagct ctttcaaata ctcgttgccg gagacgatgg tcaggtaatg attgcccgcc
     1741 agttgcccga ccttgctggc gaagcgcgcg ctgccgtagg ggaagagtat ctccgtttcg
     1801 ctcataccgc cggggtaaat gagaatctgg ccgggcgcgg gatggctggt atggttctcg
     1861 tagcccacgt tcaggtcgaa gtcgcccagt ggaatccagc aggcttcgcc gctccagcgc
     1921 gcgtgaatga ttttgttgcg aaagggcagc agcttgctga aggcggcgca tgtctgcggg
     1981 gcgtcctgct cctccatgcg cgcggtgaac acaaacggcc cggcggtgat gcgtagcaac
     2041 gtcataaaga tactccttac aatgcaggtt tcaacagttg tccaatcggc tcgctgacaa
     2101 tcttcccatc gcgatacacc gtttgcccac gcaccattgt gcgcacgatg cgcccacgta
     2161 gtgttttgcc gacataggga ctgtggtggt gacggtagaa gagcttatca gcctccaaca
     2221 cctcgtgttg ccgcaaatca accagggcca ggtcggcatc ggctccgggc gcgaactggc
     2281 cttttctggt ggatatgctg aaccgctgcg cgatattggc cgacgtcagc gacgcaacca
     2341 gggagagcgg caattgtcgc tgttcatacc cgtccgtcag cagcagttgc agcagcgact
     2401 ggcaaccgga gataccgccc cagatgcgga aaaagttggc gtcctgcttc atgctggcag
     2461 gcgcgggcga atgatcggag acgaccatcg gcagcgtccc atcgaaaata tgctgccaca
     2521 gagcatcctg ttcttgctgc gagcggatgg gaggcgcgca tttcgccacc gcgcccaggc
     2581 gaaccacatc ctcttcggtc agcgtcagat aatgcgggca ggtctcacag gtgacgtcca
     2641 cgccgtgcgc gcgcgcctcc gctatcaatg tgacgccgcg cccggagcta acatgcacga
     2701 tatgcagcgc gcagcccgtt tcggcggcga agaggatagc acgttcaatg gcctccagct
     2761 ctgccacgat gggacgcgag gcgagataat cgcgcgtgcg ggtgcgtcct tcgtcctgag
     2821 cgcgccgagc cagcgcgcct gtcagcgtgt cattttcggc gtggacggcc acgatgcggc
     2881 ccagtttcgc ggcgcgttcc atgccctcgt agagcgtcaa atcatcaacc gcctggaaat
     2941 catcgatgcc gctattactc atgaacgcct tgaagccgac cacgccgcgc tccgcgagtt
     3001 catccaaccg gtccaggttg ccaggtacca gcccgcccca tagcccgaaa tcgaccagcg
     3061 cgctcgcctg cactgcctgt aatttgagat caaagcttgc cgcatcgagc gtcggaggat
     3121 gggcgttgag cggcatgtcg aagaagcatg ttgcgcctcc cgccgcgagc gccgccgtgc
     3181 cggtggcaat gccctcccat tcggcgcgcc ccggctcgtt gaaatgcacg tgcgcgtcga
     3241 tgccgcccgg aaagatgtgc agcccggtgg catcaatctc ctcgcgcgca tctctatcca
     3301 gtccgggcgc gattgccacc aggcggccac cctcgatggc gatgtccgcc tggatggttc
     3361 cgtgcgttgt gacagccgtt ccattgcgca ccagcacgtc gtacaggctc atgtaggcac
     3421 accctcgtct ctagaaggtt ccggcgagca gcagcaaaaa gcgttcaagc gtgtcgatcg
     3481 caaggccaac atcctcgcta gtgaccgatt ccgccgggtt atggctgatg ccgcccttac
     3541 agcgcacgaa cagcatcgcc atatcggtca gcgcgctcat gatggcggcg tcgtgtcccg
     3601 cgccgctcgg caggcgatgc gccggatagc cggatgcctc gatggcctgc gcgaagagat
     3661 tggccagata tggcgcgcac ggtaccgcct tctgcgtttc gagcgcctgc caggtgaagg
     3721 tgatgtgtct tgcttcgccg atgatgcgcc ccctggcttc catctcgatc agcgcctgct
     3781 ctcgtattgt atcatgctgg tgtcgcacat ccaggctgag catcacctgc cccggtatga
     3841 cgttgctgac ccccggctgc acctgggcct ggcctatggt ggcgaccagt cccggcagcc
     3901 cgcgcgcgat ggcctccacc cccagcgcaa actcagccgc cgcgctgagg gcgtcgcggc
     3961 gcatgggcat cggcaccgtc ccggcgtggc ccgccatgcc tgtgaagcca acccgcaggc
     4021 gcgtctgtcc ggcgatggcc gtcactacac cgagcggcag attggcctcc tctaagaccg
     4081 gcccctgctc gatatgtacc tcggcatacc ccagcaatgc ctcgcgtttc cagcgaggtg
     4141 tgcgcagcgc caacgggtcg ggattgccgc cgaaatcgcg aattgcctca gccattgtca
     4201 tctcttgcgc gtctttcgcc tcaagccagg tgctctcaaa catgccggtg atggccctgc
     4261 tgcccagata cgaccgcgcg aacctcatgc cttcttcgtc cgcgaatcct accacttcga
     4321 ttgcaaatgg cagccgctcc ccgcgcgagt gcaggcgctg cacacacgcc agcgcgatca
     4381 tcacacccag cggcccatca tacttgcccg cgtcgcgtac cgaatcaaga tgcgaaccaa
     4441 gtaccagcgt tttcgcgagc gtagaactgc cctcatagcg cccaatcaag ttgccgatgg
     4501 catcctgctc aacctgcatt cccgcgtccc gcatccaggc cgatacagtg gcattcgcgc
     4561 gccgcatggc ctgcgtcgcg aatggccgcg acaggcaatc cggctcctcg ctgatcgccc
     4621 ccagaacttc gcaccattcc ataaccgtgc gcgccattgc cgcttcgcta ctcatatcat
     4681 ggccgccttt ccggttgaaa aaataattcg atgaaatttt gtctgatgga aagcgtaatt
     4741 ccgtaaagca agtatagtca caggggatgg gattgtcaag gtagttagac ccgggtctat
     4801 atctcacact gttgtttctc gactttacac caactcctcc caaagggcga ccgcaagggt
     4861 ctccctcgtg gttgtagcga agtggtttgc cacatcttac cggttgaaat tcggaataat
     4921 tcatgttata ttccgataag aggaaaatat gatttctttc aatggaaagt aggcgaagcg
     4981 aatgccgcga tgggacctgc tctcaatgcc gtcaaggaat tcgacccatt gccggatctg
     5041 gcgccggtga ggtagcgaga aaagaaggag agagatgcgt tatgtgctac aatgaggaag
     5101 agagtttttc acattatgga aagctagatg gtggcttacc cacggagggc gggagcagat
     5161 gcaaaaaata agtgaggaac aaaaggacac accgatgaat ggcgtacaat cggttgaacg
     5221 cgcgcttgac ttgatggaat gcctggggcg cgcttccggc tggttgggta tcagcgagtt
     5281 gagcagcgct acagggcagc cggttgggac gattcaccgc ctgctcaaga cgctggtggc
     5341 gcgcgagtat gttgtgcgcg atagccgcac gcgccgctat gccatcggcc ccgcttttcg
     5401 catgatggtc ggtgtgggta tgcagggacc agattgggct aaaatcggga cgcccatcct
     5461 gcgcgaatta gcctacctga gcggcgaaac ggccaacctg gcggtgatcg accgcgaccg
     5521 cgccgtctat gtcgcccagg ctcaaccggc acgtatggtg cgcatgttta ccgaaatcgg
     5581 caaccgcgtc cacc
//
LOCUS       DASZAK010000480         5838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_616244, whole genome shotgun sequence.
ACCESSION   DASZAK010000480 DASZAK010000000
VERSION     DASZAK010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616244"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..3186
                     /gene="polA"
                     /locus_tag="VGT44_08390"
     CDS             79..3186
                     /gene="polA"
                     /locus_tag="VGT44_08390"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV2580856.1"
                     /translation="MSDTSNQPEKQNTKRQTIMLIDGHAMIHRAFHAVPEDLTTSKGE
                     VVNATFGFTSILIKGLADIKPDYIAVTFDRPSPTFRHIAYPQYKAHRPALPDIMRPQF
                     GRIREVVEAFGIPIYEKDGFEADDVLGTLSVQATQLGLATLILTGDMDTLQLVNEHVK
                     VLVAKRGISDVVEYDVAQVEARYGLPPARVPDFKGLVGDKSDNIPGVPGVGEKTATKL
                     LAEYGDLENLLAHIDDLSPKEQRLLRGKEALALESKKLATIVLDAPVQLDLQASRIGQ
                     VNRERAVALFRELEFRTLVDKIPGSVSSEQAAQAIQAIEEPARPKRGEAAIAQDEELL
                     NAPPVGMVAIMQPPAPPSIASISLATTTRQGTPEQHVEVSVAVETEDAVMQPSLFGAL
                     EPPPETVRKLRLPSASSIAQSNPADLLFLDAKPSMQTSTMIVNSEESLRVLIQGIRSA
                     GIVALDTESTSEDPWHAELVGISCAMAPGEAYYIPVGHGNTLDGQEAGKQLPLAYVLE
                     QLKAVLEDENIRKYMHNAKYDMLLLLRAGVAVRGLAFDTMVGAYLTDPGRRGLGLKDQ
                     VFQRLGHVMTNITQLIGTGSEAISMARVPIRQAADYAGADADMTLRLVAPITADLKKH
                     SLLDVFYHIEMPLLPVLMQMEIFGVALNGDFLRELDGRLTEQIRALETAIYDSVGGMR
                     FNINSTKQLGDILFEQLKLPSGKKNKTGYSVSADVIESLRGKHAMVDYLLEYRQLAKL
                     KSTYIDGLLALMDPVTGRVHTTFSQTTASSGRLSSSNPNLQNIPVRTEVGRQLRRAFI
                     ADPSYVLLTADYSQFELRILAHITHEPRLVEAFTNDEDIHTITAASLFNIPVEQVTKD
                     QRRLAKTVVYAVLYGQSAFGLANITGMTNLEASEFIKRYHETFPHIKNYVDSTLTQAR
                     KQGYVNTLYGRKRFFPNMYELPFNERQALEREAINMPIQGGNADLIKIAMIRIQHDIE
                     RQRMKTRMILQVHDELVFEVPVEELDKARTLVKREMEGVAKLDVPIKVEMKIGRNWYE
                     AEPAE"
     gene            complement(3269..3874)
                     /locus_tag="VGT44_08395"
     CDS             complement(3269..3874)
                     /locus_tag="VGT44_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580857.1"
                     /translation="MIRVLLAEDQAMVRGALAALLALEGDIEIVAEVGRGDEVMVVAR
                     ATRPDVALLDIEMPGMDGIAAAALLHKELPACRVIILTTFGRPGYLRQAMQSGVVGFL
                     LKDAPSTQLATAIRRVVAGERVVDPKLAMAALSEGNNPLTEREREVLSAATLGASIAE
                     IASSLFLSEGTVRNYLSVAIQKLGAHNRIEAAHIAEQKGWL"
     gene            complement(3871..5187)
                     /locus_tag="VGT44_08400"
     CDS             complement(3871..5187)
                     /locus_tag="VGT44_08400"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV2580858.1"
                     /translation="MSNLPESDREKTTGRKGISAYLLWLIWIIWLPFFAPAFVEMFQA
                     HPPALNLILNLTGVSLFIGVYIGATLRNARNLVTPVPPSKYSSLATWLPVVVMVGLSL
                     VLTPGYGRDWFDVFIFTCAYVGGCLPARRAALVIAGIALTILLLLVVRFHDYSPFDIS
                     QLLIFSSVVGAVTMILVQSVVTTRELRAAREELTRLAVTTERLRIARDLHDLLGHNLS
                     LIALKSELAGRLVTLAPERAAAEIGDVEQVARTTLQEVREAVAAYRQPTITSELHAAK
                     ELLAAAAIDFDYQGDEEAISGLSPAIEAILAWAIREGVTNVIRHSHARHCIIRMTHTT
                     QEAGVEIIDDGATTAVPFLTQEAKASTTVGSGGSGLRGLAERVVALGGRCEAGLLPSG
                     GFRLAVVVPLAWKRRVVGEVSESAVPDSASMIASIDEQTQERGGQR"
     gene            complement(5233..>5838)
                     /locus_tag="VGT44_08405"
     CDS             complement(5233..>5838)
                     /locus_tag="VGT44_08405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV2580859.1"
                     /translation="SRIGRWRVSAGWYAVALLTPTALMLAVLFAFRTLVSPVFTPKLF
                     LPGIVFGLVPGILEEFGWMGFAFPKMRVKRSALSVAIVLGVLWGLWHAPVVDYLGAAA
                     PHGVYWPLFFLSFIAIVTAIRVLIVWIYSNTGSILLGMVMHSSLTASLAALDPAPISP
                     AQETLWYAVFAAVLWIVVVLVAARYGKSLVRQPAQVRPVSL"
BASE COUNT         1315 a         1831 c         1670 g         1022 t
ORIGIN      
        1 aaaccggcac ctacgattat tttagacagc agggagcata tacccgcatt ccggcatatt
       61 tgaaagaata ggtaatatat gagcgacacc agtaaccaac cggaaaaaca gaatacgaag
      121 cgccagacca tcatgctgat cgatggccac gccatgatcc atcgcgcctt tcacgccgtt
      181 cccgaagatt tgacgaccag caagggcgaa gtggtcaacg ccaccttcgg ctttacctcg
      241 attctgatca aagggctggc cgacatcaag cccgactata tcgcggtcac ctttgatcgc
      301 ccgtcgccca cgttccggca tatcgcttat ccgcagtaca aagcgcaccg gccagcgctg
      361 cccgacatca tgcgcccgca gttcgggcgt attcgcgaag tcgtcgaggc gttcggcatc
      421 cccatctacg aaaaagacgg cttcgaggcc gacgacgtgc tgggaacgct ttccgtccag
      481 gccacacagc tcgggctggc gacgctcatc ctcacgggcg acatggacac cttgcagctc
      541 gtcaatgaac acgtcaaagt gctggtcgcc aaacgcggca tcagcgacgt ggtggaatat
      601 gacgtggcgc aggtggaggc gcgctatggt ctgccgcccg cgcgcgtgcc ggatttcaaa
      661 gggctggtcg gcgacaaatc cgacaacatt cccggcgtac ccggcgtcgg cgagaagacc
      721 gctacaaagc tgctcgcgga atatggcgac ctggaaaacc tgctggccca tatcgacgat
      781 ctctcgccga aggaacagcg cctgttgcgc ggcaaggaag cgctggcgct ggagtcgaag
      841 aagctcgcca ccatcgtgct ggacgcgcca gtgcagcttg acctgcaggc cagccgcatc
      901 gggcaggtca accgcgaacg cgccgtcgcg ctcttccgcg aactggaatt tcgtaccctg
      961 gtcgataaaa tccccggctc tgtcagcagc gaacaggcag cccaggcgat tcaggcaatc
     1021 gaagaacctg ccaggcccaa acgcggcgaa gcggccatcg cgcaggatga agaactgctc
     1081 aacgctcctc ccgtcggcat ggtcgcgatc atgcagccac cggccccgcc ctccatcgcc
     1141 tcaatcagcc ttgcgacgac cacgcggcag ggaacgccgg agcagcatgt cgaggtcagc
     1201 gtggccgttg aaacggaaga tgctgtcatg cagccgagtc tcttcggagc gctagaaccg
     1261 cccccggaga cggtgcgcaa gctgagactt ccttccgcct catcgatcgc gcaaagcaac
     1321 ccggcagacc tgctgttcct cgacgcgaaa ccatctatgc agaccagcac gatgattgtc
     1381 aacagcgagg agagcctgcg cgtgctgata cagggcatac gcagcgcggg tatcgtggcg
     1441 ctggatacag agtctacctc ggaagacccc tggcacgcgg aactggtagg catctcctgc
     1501 gccatggctc ccggcgaggc gtactacata cccgtcgggc atggcaacac gctggatgga
     1561 caggaggccg ggaagcagct gcccctggcc tatgtgctgg agcagttgaa agcggtgctg
     1621 gaagacgaga acattcgcaa gtacatgcac aacgccaagt acgacatgct gctgctgctg
     1681 cgtgcgggcg tcgcggtacg cgggctggcc ttcgacacga tggtgggcgc gtatttgacc
     1741 gaccccggtc ggcgcggcct gggactcaaa gaccaggtct tccagcgcct ggggcatgtc
     1801 atgaccaaca tcacgcagtt gatcggcaca ggcagcgagg ccatcagcat ggcgcgggtt
     1861 cccattcgcc aggcggcgga ctacgccggg gccgacgccg atatgacgct gcggctggtc
     1921 gcgccgatca ccgcagacct gaaaaaacac agtctgctcg atgtgttcta ccacatcgaa
     1981 atgcccctgc tgcccgtcct gatgcaaatg gagattttcg gtgtcgcgct caacggcgat
     2041 ttcctgcgag aactcgacgg caggctcacc gagcagatcc gcgccctcga aacagccatc
     2101 tacgactcgg tcggcgggat gcgcttcaac atcaactcga ccaaacaact cggcgatatc
     2161 cttttcgagc aactcaagct gccttccggc aagaagaaca agaccggcta ttcggtcagc
     2221 gccgatgtga tcgagagcct gcgcgggaag cacgccatgg tcgattacct gctcgaatat
     2281 cgccagctgg ccaaactcaa atcgacctac atcgacggcc tgctggcgtt gatggacccg
     2341 gtgacggggc gcgtgcatac caccttcagc cagacgacgg cttcgagcgg gcggctctcg
     2401 tcgagcaatc ccaacctgca aaacatccct gtgcgcaccg aggtaggcag gcagctgcgc
     2461 cgcgccttca tcgccgaccc cagctacgta ctgctgacgg cagactattc gcaattcgag
     2521 ctgcgcatcc tggcgcatat cacgcacgag ccgcgcctgg tggaagcctt caccaacgac
     2581 gaagacatcc acaccatcac ggcggcctcg ctcttcaata tccccgtcga gcaggtcacg
     2641 aaggaccagc gccgcctggc caaaaccgtc gtctacgccg tgctctacgg ccaatcggcc
     2701 ttcggtctgg caaatatcac cggcatgacc aatctcgaag catcggaatt catcaagcgc
     2761 taccacgaga ccttcccgca catcaaaaac tacgtggaca gcacgctcac ccaggcgcgc
     2821 aagcagggct acgtcaacac gctctacggg cgcaaacgct tcttccccaa catgtacgag
     2881 ctgcccttca acgaacgcca ggcgctcgaa cgcgaagcga tcaacatgcc catccagggg
     2941 gggaatgctg acctgatcaa gattgctatg atccgcatcc agcacgacat cgaacgccag
     3001 cgcatgaaga cccgcatgat cttgcaggtc cacgacgagc tggtcttcga ggttcccgtc
     3061 gaagaactcg acaaagcccg caccctggtc aaacgcgaga tggagggcgt ggcaaagctg
     3121 gacgtaccca tcaaagtcga aatgaagata ggccgcaatt ggtacgaagc cgaaccggcg
     3181 gagtaaaata tcggatgcct ctctattaca ggaacgcgat gatgacaatt agatatcaaa
     3241 tcggcgccta cgcatgtggt gggcagagct aaagccagcc cttttgttcg gcgatgtggg
     3301 cggcctcaat acggttgtgc gcgccgagtt tctgaatggc gacggagaga tagttgcgca
     3361 cggtgccttc ggagaggaag agcgaggagg cgatttcggc aatgctggca cccagcgtgg
     3421 ctgccgacag cacctcgcgt tcgcgttcgg tcagcgggtt gttgccttcg ctgagcgcgg
     3481 ccatggccag cttgggatcg acgacgcgct cgcccgcgac gacgcgccgg atggcggtag
     3541 caagctgggt cgagggggca tccttgagga gaaagccgac aacgccgctt tgcatggcct
     3601 gccgcaggta gccgggccgc ccgaaggtgg tcaggataat gacgcgacac gcgggaagtt
     3661 ccttgtgcag gagggcagcg gcagcaatgc catccatgcc gggcatctcg atatccagca
     3721 acgccacatc ggggcgtgtc gcgcgtgcca cgaccatcac ttcatcgccg cgtcccactt
     3781 ccgcaacgat ctcgatatcg ccttcgagcg cgagcagagc tgccagcgcg ccccgcacca
     3841 tggcctgatc ttctgccagt aggacacgaa tcatcgctgc ccacccctct cctgtgtttg
     3901 ctcgtctatg ctcgctatca tcgatgccga atctggtact gccgactcac taacttcccc
     3961 tacaacgcgc ctcttccacg cgagcggcac cacgacggcc agccgaaagc ctccagaggg
     4021 aagcaggcca gcctcacagc gcccaccgag ggcaaccact cgttcggcga ggccgcgcag
     4081 accactcccc ccgcttccaa ccgttgtcga tgccttcgcc tcttgcgtaa ggaaaggaac
     4141 agccgtcgtt gcgccatcgt cgatgatttc aacgcccgct tcttgagtgg tgtgtgtcat
     4201 acgaatgata cagtgtcgcg cgtggctgtg gcgtatcaca tttgttactc cctcgcggat
     4261 agcccatgcc agaatggcct ctatcgcggg tgataggccg ctgatcgcct cttcatcgcc
     4321 ctggtaatcg aagtcaatag cggcagccgc caggagttcc ttggcagcgt gcaactcact
     4381 ggtgatcgtc ggttggcgat aggcggcgac agcctcgcgc acctcctgca atgtcgtgcg
     4441 cgccacctgc tcgacatcgc cgatttcagc ggcggcgcgt tccggcgcga gtgttaccag
     4501 tcgccccgcc agttcgcttt tgagcgcaat cagcgagagg ttatgcccca gcaggtcatg
     4561 cagatcgcgc gcgatgcgca gccgctcggt ggtgacggcc aggcgcgtaa gctcttcccg
     4621 cgcggcgcgt aattcgcgcg tggtcacgac ggactgcacc aggatcattg tgactgctcc
     4681 aacaactgat gagaagatca gcagttgcga gatgtcgaac gggctgtagt cgtgaaaccg
     4741 gactaccagc aacaagagaa tggtcagcgc gattccggca atcaccaatg ctgctctgcg
     4801 cgcaggcaga cagccaccaa cataggcgca cgtaaagatg aatacatcga accagtctct
     4861 cccatagccc ggtgtcagga caaggctcaa cccgaccata acgacgacgg gcagccaggt
     4921 tgcgagcgac gaatattttg agggaggcac gggcgtgacg agattccgcg cattgcgcag
     4981 ggtagctcct atgtagacac cgataaagag ggagactccg gtaagattga ggataaggtt
     5041 cagcgcaggt ggatgtgcct ggaacatttc tacaaaagcc ggcgcaaaga atggcagcca
     5101 gatgatccag atcagccaga gcagataggc agaaatgcct tttcgtccag ttgttttctc
     5161 gcgatcgctt tccggtaggt tactcaactt cctgcgcctc ctctcttcgc ttactctcac
     5221 ctttctcaca ttctacaatg aaaccggcct gacctgcgcc ggttgtcgta ccaggctctt
     5281 gccgtagcgc gcggcgacga ggacgacgac aatccacaaa acggcggcaa agaccgcgta
     5341 ccacagcgtt tcttgcgcgg gcgagatggg cgcaggatcg agtgcggcca gagatgccgt
     5401 caggctgcta tgcatcacca ttcccaacag aatactgccc gtattggagt atatccagac
     5461 gatcagaacc cttatagctg tcacgattgc gatgaaggag agaaaaaaga gcggccagta
     5521 cacgccgtgc ggggctgccg cccccaggta atcaactacc ggtgcgtgcc acaatcccca
     5581 cagcacccca agcacaatcg cgacagagag tgcgctgcgc ttcacgcgca ttttcgggaa
     5641 agcaaaaccc atccaaccaa attcttccag aatccccggc accagtccaa acacgatacc
     5701 tggcagaaaa agcttcggag taaaaaccgg ggagaccagt gttctgaaag cgaagagaac
     5761 agccagcatc agtgcagtag gagtgagcaa tgccaccgcg taccaacctg cgctaactcg
     5821 ccagcggcct atacgtga
//
LOCUS       DASZAK010000481         3142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_367547, whole genome shotgun sequence.
ACCESSION   DASZAK010000481 DASZAK010000000
VERSION     DASZAK010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367547"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2654
                     /locus_tag="VGT44_08410"
     CDS             <1..2654
                     /locus_tag="VGT44_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2580860.1"
                     /translation="FPQPQRPIANQTLAVPPPSIGASMPPVNLPAPTDAVQQGAPAGI
                     QSIEVSSNTHSNKQTMLLNRSEINIGRDPSNDVVINEGVVSGFHVQIVRKGNQFEIIH
                     PHPSRGHTLNGIIYQGQHIAGNQQFRKVLTRGDFFRIGDEHGTLVTITYNDGSGAMQD
                     IVQEIRPIPLGAPRITIGRQLDNDLVLAHPQVSSHHAYLEQVQGGYRIVDLNSTNHVY
                     VNGQRVSSQLLQPGNEIRIGPYNLTYTGTQLTQRDESGGVRIDAFQLHKEGNNNTILI
                     NDISLAIPPRKFVALVGGSGAGKSTLMDALNGLRPAQKGGVFYNGKDYYRHLAAFSTQ
                     LGYVPQDDIIHRDLTVERALYYTARMRLPDDFTEEQIKQRIDEVLDDVEMKFRRNLLV
                     SKLSGGQRKRVSIALELLANPSIFFLDEPTSGLDPGLDRKMMFLLRKLADKGHTIVLV
                     THATNNINTCDYICFLAQGGRLAYFGPPNEAKSYFQKTDFAEIYSALEPTEQNPDIPK
                     HAEEQFKQSPLFQQFVVAPLNQSPATQAQLNRQQQPAEVIPPKRGDPWKQFSLLSRRY
                     LELLRNDRGNLLILLLQAPLIALILAFMLLGGGHGTFDPTTIAKCNLNPPGTNVHIPR
                     SPDATNQFDCISVQQSLQNSGNPNPLQTLQQFIEPGSGGDAQKYLFLMAFTALMFGCI
                     NGSREIVKEAPIYRRERAVNLGIAPYLFSKIAVLGILCLFQSAILVLVVNVVAPIQQG
                     IVLPATVEVYITMALTSLSGLMLGLMVSALAPNNDRAVSSIPILLIPQVIFSGLLFQI
                     NSPVLQVISSVFAVRWGLAAAGSSVGIHGDKIGSDTWTYQGTLFSTNSQAAATFHLLL
                     CWFFLVAMIVIFGILTTYFLKRKDVRV"
     gene            2911..>3142
                     /locus_tag="VGT44_08415"
     CDS             2911..>3142
                     /locus_tag="VGT44_08415"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HEV2580861.1"
                     /translation="MLLNRRVIVGICGGIASYKAVELVSQLQQAGTLVDVIMSERAAE
                     FVRPLTFSALSHRPVYTDLWEPSGRAAETHIAL"
BASE COUNT          687 a         1032 c          793 g          630 t
ORIGIN      
        1 cgttcccgca accgcagcgc cctatagcaa accagacgct cgccgtcccg ccaccgtcaa
       61 taggcgcaag tatgccgccc gtcaacctgc cagctcctac cgacgcggtt caacagggcg
      121 cgcctgctgg catccagtca atcgaagtca gctcaaatac tcattccaac aagcagacga
      181 tgctgttgaa caggtcggaa atcaatatcg gacgcgatcc atcgaacgac gtggtgatta
      241 acgagggagt cgtctccggt ttccatgtcc agattgtgcg caaaggcaac cagttcgaga
      301 ttattcaccc acatccctcg cgcggacata cgctcaacgg tattatttat caaggccagc
      361 acattgcggg caaccagcaa ttccgcaaag tattgacgcg cggcgatttc ttccgcatcg
      421 gcgacgaaca cggtactctc gtcacgatta cctacaacga tggcagcggc gccatgcaag
      481 acattgtgca agaaatccgc cccatcccgc tcggtgcgcc aagaatcacc atcggacgac
      541 agctggacaa tgatcttgtg ctggcccatc cccaggtctc cagccatcac gcctacctcg
      601 agcaggttca ggggggctat cgcatcgttg acctgaacag cacgaaccat gtctacgtca
      661 atggccagcg tgtgagctct caactgcttc aaccaggcaa cgaaatccgc atcggccctt
      721 ataacctgac ctataccggc acacaactga cgcagcgcga cgaaagcgga ggcgtgcgta
      781 tcgacgcctt tcagctgcat aaagagggca acaacaacac gattcttatc aacgatatct
      841 cacttgccat tcccccgcgc aaattcgtcg cgcttgtggg cggttcaggc gcgggcaaat
      901 caacgcttat ggacgccctc aacggcctgc gccccgcgca aaaaggcgga gtcttttaca
      961 acggcaagga ctactaccgt catcttgccg ccttcagcac tcagctcggc tacgtgccgc
     1021 aggacgacat catccaccgc gacctgaccg tcgaacgcgc cctctactat accgctagaa
     1081 tgcgcctgcc cgacgacttt accgaagagc agatcaagca gcgcatcgac gaggtgctgg
     1141 atgacgtaga gatgaaattc cggcgcaacc tgctcgtcag caaactctcc ggcggccagc
     1201 gcaagcgcgt ctctatcgcc ctcgaactgc ttgccaatcc gagcatcttc ttcctggatg
     1261 agccgacctc cggcctggac cccggcctgg accgcaaaat gatgttcctg ctgcgcaaac
     1321 tggccgacaa aggccacact attgtgctgg tgacccacgc caccaacaac atcaacacct
     1381 gcgactacat ctgtttcctc gcccagggtg gtcgcctggc ctatttcggc ccgcccaacg
     1441 aggccaagtc ctatttccag aaaaccgact tcgccgaaat ctacagcgcg ctggagccaa
     1501 ccgagcagaa ccccgacatt ccaaaacatg ctgaggaaca gttcaagcaa tcgcccttgt
     1561 tccaacaatt cgtcgtcgcc ccgctcaatc agagccccgc gacccaggcg cagttgaacc
     1621 gccagcagca gccagccgag gtcattccac ccaaacgcgg cgacccctgg aagcagttct
     1681 ctctgctctc ccggcgctat ctcgagctgc tcaggaacga ccggggcaac ctgttgatcc
     1741 tgctcttaca agcgcccttg atcgccttga ttctggcctt tatgctgctc ggcggcggac
     1801 acggcacctt cgacccgact accatcgcga aatgtaacct caacccgccc ggtacaaacg
     1861 tccatatccc tcgctctccg gacgccacga accagtttga ctgcatcagc gtacaacagt
     1921 ccctccaaaa ctccggcaac ccaaacccgt tgcagacgtt gcaacaattt atcgagcctg
     1981 gcagcggcgg tgacgcgcag aagtacctgt tcctcatggc cttcacagcc ctcatgttcg
     2041 gctgcatcaa cggctcacga gaaattgtca aagaagctcc catttatcgc cgcgagcgcg
     2101 ctgtgaacct gggcattgcg ccctatttgt tctccaaaat tgccgtcctg ggcatccttt
     2161 gcctgtttca gagcgccatc ctcgtgctgg tcgtcaacgt ggtcgcgccc atccagcagg
     2221 gcatcgtcct ccctgccacc gtggaggtct acatcacaat ggcgctgacc tctctgtccg
     2281 gcttgatgct cggcctgatg gtctccgcgc tcgccccgaa caacgaccgg gccgtcagct
     2341 ccatcccgat tctcttgatc ccccaggtca ttttctccgg tttactgttc cagattaaca
     2401 gcccggtttt gcaagtcatc agctcggtct tcgccgtgcg ctggggcctg gcagccgccg
     2461 gctcaagcgt tggcatccac ggcgacaaga tcggctccga cacctggacc taccagggaa
     2521 cgctcttctc aaccaacagc caggctgccg cgaccttcca ccttctgctc tgctggttct
     2581 tcctggtcgc gatgatcgtc atcttcggca tactgacaac ctacttcctg aaacggaagg
     2641 atgtgcgcgt ttagcagatg tgaaggacga ccctgccgac cagaaatgtc accctgagcg
     2701 cagcgaagga tctctgtccc tggcagtcag accctacgct gcgctcaggg tgacaatcga
     2761 cgacaggccc cgaggcgctc gccctggcca tcccggtatg gtacttctgg gcgatgcgct
     2821 tgtgaagatg tgtcaaccta tgctattgtt gcattacctc gtgacgcaag gcggcaagag
     2881 gcgcgagtaa agggaacggg caagcaagcg gtgttactta acagacgggt tatcgtcgga
     2941 atatgtggcg gcattgcatc gtataaagcg gtcgagctgg tcagtcaatt gcaacaggcg
     3001 ggcacgctgg tggatgtgat tatgtcggag cgcgcggcag aatttgttcg cccgttgaca
     3061 ttctctgcgc tgagtcatcg accggtctat accgatttgt gggaaccttc tgggcgcgcg
     3121 gcggaaaccc acatagccct gg
//
LOCUS       DASZAK010000482         1733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_509484, whole genome shotgun sequence.
ACCESSION   DASZAK010000482 DASZAK010000000
VERSION     DASZAK010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1733
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509484"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..784
                     /locus_tag="VGT44_08420"
     CDS             128..784
                     /locus_tag="VGT44_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV2580862.1"
                     /translation="MLNLTPDELLTTTRSVRKRLDFSRPVEPELLRECLEIAVQAPTG
                     GNRQHWHFIIITDPRQIAALGAIYKKGWAIYIQEQTSVANAAQSRRMTPDRLSTLARV
                     ADSAIYLSEHMGEVPAMLIPCAYGRIDNVPSSAQAGYWGSLLPAVWSFMLAARARGLG
                     TCWTSVHLNYEQEAAQVLGIPYDKVAQGALIPVAHTLGSDFKPGSRVPLDEILHWDRW
                     "
     gene            918..>1733
                     /locus_tag="VGT44_08425"
     CDS             918..>1733
                     /locus_tag="VGT44_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580863.1"
                     /translation="MSTLKIPVNRGKTSLLTSLSLLGLLLLLVSACADSSPTTTATTP
                     TASTTTGASQPGDFGLAALPGYQVSLFARQTSAFNNPDSLVVDNGFVYIDYQNTTAKD
                     CTDKNTSTIVQYDMNGKMLKTFVVPGHSDGMRADPSTHLLWVTSCEDGNPKFVTIDPT
                     SGTITPYTFPPTPHGGGYDDLCFLNGLVFIAASNPNVNSAGVNVFPAIDKITLGNGKV
                     NLTPVLMGNATATDLIASAKTTLNEVDPDSMTVDSSGQLVLVNQAGSELVFLKN"
BASE COUNT          404 a          528 c          437 g          364 t
ORIGIN      
        1 gccaagctca cggctcacag cgtggggatg atggtcaacc agctggtggg acggcctccg
       61 ttacgcctgg cggacttggc cgtctagctc ttctgtgaac gaggaactcg cacaaagcgt
      121 ctatatcatg ctaaatctca cacccgatga attgctcacc accacgcgct ctgtgcgcaa
      181 acggctcgac ttttcgcgcc cggtcgaacc cgaactgttg cgcgaatgcc tggaaatcgc
      241 ggtacaggcg cccaccgggg gcaaccgcca gcattggcat ttcatcataa tcacagaccc
      301 ccggcaaatc gcggcgttag gagcaatcta taagaaaggc tgggccatct acatccagga
      361 gcagacctcc gtcgccaatg ccgctcagag tagacgcatg acgcccgacc gtctctccac
      421 gctcgccagg gtcgccgact ccgccatata cttgtcagaa cacatgggcg aagtcccggc
      481 catgctgatc ccctgcgctt atggccgcat cgataacgtc ccatcgagcg cgcaggcagg
      541 ctactggggg tcgctgctgc ccgccgtctg gagcttcatg ctggcggccc gcgcgcgcgg
      601 cctgggaacc tgctggacgt ccgtccatct caattacgag caagaggccg cccaggtact
      661 cggcatcccc tacgacaagg tggcgcaggg cgcgctcatt ccagtagccc ataccctcgg
      721 cagcgatttc aaacccggct cgcgtgtgcc attggacgaa atcctgcact gggaccggtg
      781 gtaaattccg tgtctacatt aaaggctatc ttaaccaggc gataacatca gcttaacatt
      841 gtggaagtat gttcttcgta aggacaggaa actttacctg ctcctgtatc tttcatggaa
      901 agaaaacaga ggaacatatg tccactctca aaatcccggt taatagaggg aagacatctc
      961 ttctcacgtc gctctctcta ttaggactgc tcttgctgct cgtgtcggcg tgtgcagact
     1021 catcgccgac taccacagca acaaccccaa cggcaagcac cactacaggc gcatcacagc
     1081 ccggcgattt tggcctggcg gcgcttcctg gctaccaggt ctcattattt gctcgccaga
     1141 cctctgcctt taacaatcct gactcacttg ttgtagacaa cggcttcgtc tatatcgact
     1201 atcaaaacac gacagcgaag gactgcaccg acaaaaatac cagcaccatt gttcaatacg
     1261 acatgaacgg gaaaatgctt aaaacgttcg tagtgcctgg acatagcgac gggatgcgcg
     1321 ccgatccctc gacacatctg ctctgggtga catcctgtga agacggcaac ccgaagtttg
     1381 tcaccatcga tccaaccagc gggaccatca cgccgtacac cttcccacct acgccacatg
     1441 gcggtggtta tgatgacctc tgcttcctca atggccttgt attcatcgcc gcttcgaatc
     1501 caaatgtgaa ctcagccggc gtgaatgtct ttccagctat tgacaagatc accctgggca
     1561 atgggaaagt gaatctgaca cccgtgttga tgggcaatgc aactgccacc gacctgatag
     1621 ccagtgcaaa gacaacgttg aacgaggtag accccgattc gatgacggtg gattcatcag
     1681 gacaactggt tctcgtgaac caggccggct ccgagctggt gtttttgaag aat
//
LOCUS       DASZAK010000483         2869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_261186, whole genome shotgun sequence.
ACCESSION   DASZAK010000483 DASZAK010000000
VERSION     DASZAK010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261186"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="VGT44_08430"
     CDS             <1..1268
                     /locus_tag="VGT44_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580864.1"
                     /translation="APIRRRRVIAPAPQPARTTLKHNVRGGRKRGRTALLVSVALLIP
                     VVLLILLALSRVFGVYLLPANIPLVGAPVATVSVTVKSQTISNSYLLMASPGISQPDV
                     TTRALPDRVLSVVVSDSKAEPVSGTQTIAGKQASGTLLFDNSIHQTVFEQQGAQFTAS
                     NGVVVALAQAVSIPPRSNGQDGAATAPAIALENGANGNIAANELFTTCCNGLTISNPQ
                     PFTGGVDASVVHIVTQADLDNARNALSPGLQQKALQQLQGQLVSGEVEAGKPTISMQV
                     TSDNPVGAKVDTINVQVHLTAKVIAYNAESARQVAEELVIRQAAQQLGNDYQLKDTLV
                     VAAPVVTSQESSGVIYLTVSARGVWLYHLTQQQVDQWRQAIRDSTLQLAQTYITTQAG
                     VAGVKIQLPFGMDHLPSSLNQIQIVLVNE"
     gene            1363..2766
                     /locus_tag="VGT44_08435"
     CDS             1363..2766
                     /locus_tag="VGT44_08435"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEV2580865.1"
                     /translation="MSTTDARVGEMQYYQSMLDGIGRTPLIKLNRVAEDVAPLMLVKM
                     EMFNPGGSVKDRIGPAMIEYCEKLGVLRPAGTIVEPTSGNTGHGLAIAAAIKGYHCIF
                     VMTDKVSEEKRSLLRAYGAEVVICPSSVTHDSPEHYKNVAHRLAREIPGACCPDQYSN
                     PANPAAHYATTGPEIWRDTAGYVTAFVAGIGTGGTISGVARYLKEQNPNIKIIGADPP
                     GSVFSGDTPGPYKVEGIGMELVPENYDASLVDEVIRVDDRSSFYWARRLAREEGILAG
                     GSAGTALAAALIYAHRLTADDVVVTLLPDTGRGYLSKQFNDTWMRENNLLVQTETDKP
                     TLRDVLTYRRSQGRTMPLVVSVTPTDSIADAVELLRRYGISQTPVMENGRMVGSVTEN
                     LLLRHLASDEPLASMVVRDLQGAPFRQLPGDALAQEAYTLFSSGQPAVAVMHGDLLEG
                     IITQSDLLEFWAHTKSA"
BASE COUNT          627 a          811 c          870 g          561 t
ORIGIN      
        1 aggctccgat caggcgaagg cgtgtcattg cgcctgcccc tcaacccgcc cgtacaacgc
       61 tcaagcacaa cgtgcgtgga gggcgcaaac gcggacgcac tgcgctgctg gtgtcggtcg
      121 cgctgctgat tccggtcgtg ctgttgatcc tgctagctct gtcgcgcgta tttggcgtgt
      181 atctccttcc cgcgaacatt cctctggtcg gcgcgcctgt cgcgaccgtc tcggtcacgg
      241 tcaaaagcca gacaatctcc aatagctatt tactgatggc gtcgcctggc atctcgcaac
      301 cggatgtcac gacgcgcgcg ttaccggatc gtgtcctttc ggtagtggtg agtgacagca
      361 aagcagagcc agtatcgggg acgcagacca tcgccgggaa gcaggcttcc ggtacgctct
      421 tgttcgataa cagcattcac cagaccgtct ttgaacagca gggggcgcaa ttcacagcaa
      481 gcaacggagt agtggtggcg cttgcgcagg cggtatcgat tcctccgagg agcaatggtc
      541 aagatggcgc cgcgaccgcc ccggcgatcg ctctggaaaa tggcgcgaac ggcaatattg
      601 ccgcaaatga attgttcact acatgctgca atggccttac aattagcaat ccgcaaccat
      661 ttaccggagg cgttgatgcg agtgttgttc atatagtcac acaggcagat ctcgataacg
      721 cgcgcaatgc cctcagccct ggcctgcagc agaaggcgct gcaacagctc cagggccaac
      781 ttgtatctgg cgaagttgag gcaggcaagc caacgatctc aatgcaggta acatcggata
      841 accctgtagg agcgaaggta gatacgatca atgtccaggt gcatctcaca gcgaaggtca
      901 tagcctataa cgccgagtcg gcgcgccagg ttgccgagga gctggtaatc cggcaggccg
      961 cgcaacaact gggcaatgat taccagctca aagatacgct ggtcgttgcc gcgccggtcg
     1021 tcacctcgca ggaatccagc ggcgtgatct atctcaccgt ctccgcgcgt ggcgtctggc
     1081 tgtatcacct cacacagcag caggtcgatc agtggcgaca ggcgatcagg gactcgacct
     1141 tgcagcttgc ccagacctat ataacaacgc aggcgggggt tgcgggcgtc aagattcaac
     1201 tcccgtttgg catggatcat ttgccttcat cgctcaacca gattcagatc gtgctggtga
     1261 atgagtagca gggtgaggtt ccagagggcg accacaaggg tgtgctatac taactccgtt
     1321 aaactctggt tatgagcgct atattccgaa aaaggaacgt caatgagtac aaccgatgct
     1381 cgtgtaggtg aaatgcaata ttatcaatcg atgctggatg ggattggccg gacgccactt
     1441 atcaagctca atcgcgtcgc ggaagatgta gcgccgctga tgctggtgaa gatggaaatg
     1501 tttaatcctg gaggaagcgt caaggaccgc atcggaccgg ctatgatcga gtattgcgag
     1561 aagttgggag tgctgcgccc cgccgggacg attgtcgagc caacgagcgg caataccggg
     1621 catgggctgg cgattgctgc cgcgatcaag ggctatcatt gtatcttcgt catgaccgac
     1681 aaagtgagcg aggagaagcg cagcctgctg cgcgcctacg gcgcggaggt ggtgatttgc
     1741 cccagcagtg ttacgcacga ttcgccggag cattacaaga acgtggcgca tcgtctcgct
     1801 agagagattc ccggcgcgtg ctgccccgat caatactcca accccgccaa tcctgccgcg
     1861 cattacgcca ccaccggacc cgaaatctgg cgcgatacag cagggtacgt gacagctttt
     1921 gtggcgggta taggcactgg cgggacgatc tcaggtgtcg cgcgctacct caaggaacag
     1981 aatccgaata tcaaaatcat cggcgcggac ccgccaggat cggtcttctc cggcgataca
     2041 ccagggccgt acaaagtcga aggcatcggt atggagcttg ttcctgagaa ttatgatgcc
     2101 tcgctagtgg atgaggtcat acgtgtggat gatcgcagct cgttttactg ggcgcgccgc
     2161 ctggcgcgcg aagaaggcat cctggcggga ggttcagccg ggaccgccct ggcagcagcg
     2221 ctgatttacg cgcaccggct gaccgccgac gacgtggtgg tgacgctgct gccagatacc
     2281 gggcgaggct atttgagcaa gcagttcaac gatacctgga tgcgcgagaa taacctgctg
     2341 gtgcagacag aaacggacaa acccaccttg cgcgatgtcc tgacctaccg ccgctcgcag
     2401 gggcgcacga tgccgctggt cgtcagtgtt acgccgaccg attcgatcgc agacgcggtg
     2461 gagctgctgc gccgctacgg catcagccag acgccggtga tggagaatgg caggatggtc
     2521 ggcagcgtca ccgaaaacct cctgctgcgt cacctcgcca gcgatgagcc gctggcatcg
     2581 atggttgtgc gtgacctgca aggcgcgccc ttcagacaat tgccgggcga tgccctggcg
     2641 caggaagcct acacgctctt tagctccggc caaccggcag tggccgtgat gcatggcgat
     2701 ctgctggaag gcatcatcac gcagagcgac ctgctagaat tctgggcgca cacgaagtcc
     2761 gcctaaagtc cgacaaggat gtagaggagc aattcattgc gccctggtgg acgcgcttga
     2821 cagggagcga gggcgcaatg aattgctcct ctacatcctt gtcggacta
//
LOCUS       DASZAK010000484         5195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_225117, whole genome shotgun sequence.
ACCESSION   DASZAK010000484 DASZAK010000000
VERSION     DASZAK010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225117"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            312..1421
                     /locus_tag="VGT44_08440"
     CDS             312..1421
                     /locus_tag="VGT44_08440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2580866.1"
                     /translation="MPSILPIDPVSYPLGVICVLLMTLQFCLHWICLSGKLQQRWYWF
                     YFLLQGVCVLIISFIWPDTILALGLYLVLVIEALSMLKQVQQTVGVGATCLLLFILTI
                     AWQLTQVAIWMTFESVLLLILFIIGWIILLIQQAHTRSQLEAAHLQLAAYAMRIEELT
                     LVNERQRLARELHDTLAQDLVGLTLQLERVDLHLEKKRVERARELVQQAMSRSRATLA
                     EARSAIDDLRATTLNPNDLLLAVQREINRFRATTSISCVADINALSTVPESLCEHVLR
                     AITEGLTNVARHSQAHQVWICARLDAAMLTIEVRDDGIGFDNKEIVTHKGHYGLLGLR
                     ERARLSGGNLDIESAVGKGTLLRLRLPINRRGGLD"
     gene            1418..2107
                     /locus_tag="VGT44_08445"
     CDS             1418..2107
                     /locus_tag="VGT44_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580867.1"
                     /translation="MTEEKTEVRIVVADDHLVVREGLRFMLEEADEGFVLVGDAGDGA
                     TALDVVGKVQPDVVLMDLRMPEMDGLEAIKRIRTAWPHISVLILTTYNEDDLMIRGLQ
                     VGACGYLLKDTDRATLFRAIRAAARKEMAVQPEMMARILSHAAHHVSTSPVSPSNNTR
                     RESDLTDRELEVLESVVRGERNKEIAKRLGVTERTVRAHLSTIYMKLNVDSRAQAVAV
                     ALKHGLILPLT"
     gene            complement(2134..2889)
                     /locus_tag="VGT44_08450"
     CDS             complement(2134..2889)
                     /locus_tag="VGT44_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEV2580868.1"
                     /translation="MLTAERRRSIMQALQRDGQVLASELSRSLHVSEDTIRRDLRELA
                     AAGKLQRVHGGAMPRSPDIASFTERQERAADAKEAIARAALRFIRQNQVIILDGGTTP
                     LQVAQRLPADLRATIITHSPPIALALAEYESIEVILIGGKLLKRTLVTVGAATVEEFR
                     HIRADLCFLGVSGLHPEVGISTRDLEEAYVKRAMIDSSAEVVALASNEKLGLASPYIV
                     GSLSKLTHLITERSVSNEILEPYREAGIAIVRA"
     gene            3106..3300
                     /locus_tag="VGT44_08455"
     CDS             3106..3300
                     /locus_tag="VGT44_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580869.1"
                     /translation="MNETFKQISFQPSLPCVYHDCHEQATTAIAYAVSEREWRIVPTC
                     EQHSVLFPKNHEQPAEAASR"
     gene            3332..3787
                     /locus_tag="VGT44_08460"
     CDS             3332..3787
                     /locus_tag="VGT44_08460"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF003559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEV2580870.1"
                     /translation="MALVAHDDKKHDLLEWARYNKVLLTHHELYSTGTTGKLLEQELG
                     LRINKLQSGPLGGDQQVGAKIADGEIDLLVFFWDPLTTQPHDSDVRALLRIAVVWNIP
                     VACNRASADFMIASPLMAGSYQPSSPDYHRYLERMDNELDEERLVSPAV"
     gene            3806..4570
                     /locus_tag="VGT44_08465"
     CDS             3806..4570
                     /locus_tag="VGT44_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEV2580871.1"
                     /translation="MEGIKNYLTDMDGVIVRGPTLIPGAVEFVQRLLAREIPFLILTN
                     NSLYTPRDLQMRLSYMGLEVPAEAIFTSALATAQFLHAQHPGGRAYAIGESGLTTALH
                     DIGYILTDQEPEYVVLGETTTYSFERITRAIRFVSAGARFIATNPDVSGPGEGGLVPA
                     TGAVAALISAATGVKPYYIGKPNPLMMRTALRTLNAHSEDSVMIGDRMDTDMISGMES
                     GLRTILVLTGVTSREQVERFPYRPTWIRESIADVEI"
     gene            complement(4633..>5195)
                     /locus_tag="VGT44_08470"
     CDS             complement(4633..>5195)
                     /locus_tag="VGT44_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEV2580872.1"
                     /translation="FVTQQNWEAWLNEHHAESGGIWLKIAKKRAGSMSVSYAQALESA
                     LCYGWIDGQKGSLDDQYWLQKFTPRRAKSIWSKVNCVKAETLIAQGRMQAAGLRQVEL
                     AKADGRWDQAYASQSNITIPDDFQSELDKNPEAKVFFSTLDRINRYAILFRIQTAKKA
                     ETRVARIQKFIDMLSKHETIYPISSR"
BASE COUNT         1123 a         1487 c         1421 g         1164 t
ORIGIN      
        1 gagcagctcc ttcgtaagac actatgggca aacggatgcg cgggttgctg aatttcgaaa
       61 catatcagaa aattatctgg gacaccgctg agaatgatct gagccactac agacaggaca
      121 tacgccctat ccgctggttt atcctgttct gggtgggcat tgttttcctg tggggaagcg
      181 cgcaattgct cctgggagtc tatttgaggc tcccgttcgg agggttcctc attagctcga
      241 aaatctttca gccttcgcag tcgccaggtc aaacatttcc agtaaaggct tctttcctac
      301 gagggaatac cttgccgagc atcttaccga tcgatccggt atcgtatccc ctgggggtga
      361 tctgcgttct gttaatgacc ttacaatttt gcctccactg gatctgcctt tcgggcaagc
      421 tgcaacagcg ctggtactgg ttctactttc tgctccaggg agtatgtgtc ctcattataa
      481 gtttcatctg gcccgatacg atcctcgcgc ttggtctcta cctggtgctg gtcattgaag
      541 cgctcagtat gctcaagcag gtgcaacaga ccgtgggagt gggggcaacc tgcctgctgt
      601 tattcatcct gaccattgca tggcaactga cgcaggtggc gatttggatg acgttcgagt
      661 cggttctgct gcttattctc ttcattatcg gatggatcat tctcttgatc caacaggccc
      721 atacacgcag ccagctggag gcagctcatc tccaactggc tgcctatgct atgcgcatcg
      781 aagaactgac cctggtcaac gagcgccaac ggctggcgcg ggaattgcat gataccctgg
      841 cgcaagacct ggtgggactc accttgcaac tcgagcgggt cgatctgcac ctcgagaaaa
      901 agcgcgttga gcgagcgcgc gagctggtgc agcaggcgat gtcacggtca cgcgcaacgc
      961 tggctgaagc ccgcagcgcg attgatgatc tgcgcgcgac gacgctcaac cccaacgacc
     1021 tgctcctggc ggtccagcgg gagatcaacc gctttagagc gacgacatcc atctcctgtg
     1081 tggctgacat caacgcgctt tcgacggttc ccgaaagtct gtgcgaacac gtgctgcggg
     1141 cgatcaccga aggactgacg aatgtcgccc gtcactccca ggcccaccag gtctggatat
     1201 gcgcgcgcct cgatgcggcc atgctgacaa ttgaagtgcg cgacgacggg attggctttg
     1261 ataataagga aattgttacc cacaaaggac actatgggct gttaggattg cgcgagcgcg
     1321 cgcgcctcag cgggggcaat ctcgacattg agagtgctgt ggggaaaggg actctgctcc
     1381 gtttgcgcct accgatcaat aggagaggag gtctggattg actgaagaaa agacagaggt
     1441 ccggatcgtg gttgccgatg atcaccttgt tgtacgtgag gggctgcgtt tcatgctgga
     1501 ggaggcggac gagggatttg tcctcgtcgg tgatgctggc gatggcgcga cagcgctcga
     1561 cgtggttggc aaagtacaac ccgatgtcgt attgatggat ctacgtatgc cggaaatgga
     1621 tgggttggaa gcgatcaaac gaattcggac agcctggcct catatctccg ttcttatttt
     1681 gacgacctac aatgaagatg acctgatgat acgcggcctg caagtcgggg cctgtgggta
     1741 cttgctcaaa gataccgatc gcgcgacact cttccgtgcc atccgggcgg cagcgcgcaa
     1801 agaaatggcg gtacaaccgg agatgatggc ccgcatcctc tcccacgcgg cgcatcacgt
     1861 ctcaacatcg cccgtctcac cgtcaaataa cacccgcaga gagtcggatc tgacggatcg
     1921 cgaactggag gtactggaaa gcgtcgtgcg gggtgagcgc aacaaagaga tcgcgaaacg
     1981 tctgggcgta accgagcgca cggtacgcgc gcatttgagc accatctata tgaagctgaa
     2041 cgtagactct cgcgcccagg ctgtggctgt cgccctcaaa catggcctga tcctccctct
     2101 aacgtagcgc acccccctct ctcacctgtt ctcttacgca cgtacaatcg caatgcccgc
     2161 ctccctgtac ggctccaata tctcgttcga cacagagcgc tcggtgatga gatgcgtcag
     2221 cttgctgagg gagccaacga tatacggtga ggccaggccg agcttttcgt tcgaggcgag
     2281 cgccaccact tcggcagaac tgtcgatcat ggcgcgttta acataggctt cctccaggtc
     2341 gcgtgtgctg atacccactt caggatgcag gccactgacg cccaggaagc acagatcggc
     2401 ccggatatgg cgaaactcct cgacagtcgc agctccgacc gtcacgaggg tgcgtttgag
     2461 aagctttcct ccgatcaata tgacctcaat gctttcatac tcggccagcg ccagagcaat
     2521 aggagggctg tgagtaatga ttgtggcgcg caggtctgcc ggtaggcgct gtgccacttg
     2581 caacggcgtc gtgccgccat ctaagataat cacctggttc tggcggataa agcgcaaggc
     2641 cgcgcgcgca atggcctcct tcgcatcggc agcccgttcc tgccgctcgg tgaagctggc
     2701 gatgtcggga gagcgtggca tcgccccgcc atgcactcgc tgtaacttgc ccgccgcagc
     2761 cagttcgcgc aggtcgcgcc tgatggtatc ttccgagacg tgaagagatc tgcttaactc
     2821 cgatgcaagc acctggccat cgcgttgcag cgcctgcata atagaacgtc gtcgttctgc
     2881 cgtcaacatc gttgcgttat tctccttgat attgcatgat actgcttgac attgcatgag
     2941 attgtgtggt atcatcctgt acgtgcagta tattgcacgt ttgtagcggt tgtcaatacc
     3001 acagtcaggt tttgcatagt atccagaagg cgacaaggaa cgccctccag gcaccaggat
     3061 taagagtaag taacagtttc accacgaaag tgagaaaata taaacatgaa cgaaacattc
     3121 aagcagatca gctttcagcc gtcgctgccg tgcgtttacc acgattgcca cgagcaggcc
     3181 acgaccgcga tcgcttatgc cgtctctgag cgcgagtggc gcatcgtccc tacttgcgag
     3241 cagcacagcg tcctgtttcc caaaaaccac gagcagcccg cagaagccgc aagccgctaa
     3301 aggagcattt cccatcgaac atatcaaaca catcgccctg gtcgcacatg atgacaaaaa
     3361 gcatgacctt ttggaatggg cgagatataa taaagtgtta ctaacccatc acgaactgta
     3421 ctcgaccggc acaaccggca aactgctcga gcaggaattg ggtttacgca tcaacaaact
     3481 acaaagcggc ccactgggcg gcgaccagca ggtaggcgcg aaaatcgccg acggcgagat
     3541 cgatttgctc gtcttcttct gggacccgct tacgacacag ccgcacgact cggatgtgcg
     3601 agcgctcttg cgcattgccg tcgtctggaa tattccggtg gcctgcaacc gcgcgagcgc
     3661 cgatttcatg atcgcgtcgc ctttgatggc cggatcgtac cagccatcct cgcccgacta
     3721 ccatcgctac ttagagcgca tggataacga gcttgatgaa gagcgactgg tttcgccagc
     3781 cgtgtaatct attggaggga aggccatgga gggcatcaag aattacctga ccgatatgga
     3841 tggcgtgatc gtgcgaggtc cgactctgat ccccggcgcg gtggaatttg tacagcgcct
     3901 gctggccaga gaaatcccgt ttctgatcct gaccaataac tcgctgtaca cgccgcgcga
     3961 cctgcagatg cgcctatcct acatgggatt ggaggtgcct gccgaggcga ttttcacctc
     4021 ggcgctggcc accgcgcagt tcctccacgc acagcatcca ggcgggcgag cgtatgccat
     4081 tggcgagtcc gggttgacaa ccgcgctgca cgacatcggc tatatcctga ccgaccagga
     4141 gcctgaatac gtcgtgttag gcgaaacgac cacgtacagt tttgaacgca tcacgcgcgc
     4201 catccgtttt gtatcggctg gcgcgcgttt cattgccacc aatcccgatg tatctggccc
     4261 cggcgagggc ggccttgtcc cggccaccgg cgctgtagcc gcgctaatca gcgccgccac
     4321 cggtgtcaag ccctactaca tcggcaagcc caacccgttg atgatgcgca ccgccctgcg
     4381 tacgctcaac gcccattccg aggactccgt gatgatcggc gaccgcatgg ataccgacat
     4441 gatctccggc atggagagcg gcctgcgcac gatcctggta ctcaccggcg tcacctcgcg
     4501 cgagcaggtg gaacgcttcc catatcgccc tacctggatt cgcgagtcca tcgcggacgt
     4561 agagatctaa aagagttcaa caccgtaggg ccggttttcc gcgctccctt ctacggcgtt
     4621 tgttcttaaa ccttatctgg acgaaatagg atagattgtc tcatgcttcg agagcatgtc
     4681 aataaacttc tggatacgag ccacacgtgt ttcggctttc ttcgccgttt ggattctaaa
     4741 caggattgca tatcgattaa tcctatccag tgtgctgaaa aaaactttcg cttcaggatt
     4801 cttgtccagt tcgctttgaa aatcatcagg aattgttatg ttactctgcg atgcgtaagc
     4861 ctgatcccaa cgcccatcgg ccttcgctag ctccacttgc cgcaatcctg ccgcttgcat
     4921 cctgccctga gcaatcagcg tctctgcttt cacgcaattg acttttgacc agatactctt
     4981 tgcgcggcga ggagtgaatt tttgtagcca gtactggtcg tccaacgagc ctttttgccc
     5041 atcgatccag ccataacata gagcgctctc cagggcttga gcatatgaga cagacatgga
     5101 gcctgcccgt tttttagcaa tcttgagcca gatgccgcct gactcggcat ggtgttcatt
     5161 gagccatgct tcccagtttt gctgggtcac aaatg
//
LOCUS       DASZAK010000485         3616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_83395, whole genome shotgun sequence.
ACCESSION   DASZAK010000485 DASZAK010000000
VERSION     DASZAK010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3616
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83395"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1508)
                     /locus_tag="VGT44_08475"
     CDS             complement(<1..1508)
                     /locus_tag="VGT44_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2580873.1"
                     /translation="MLLEGMQLGRYRLAQLLGTGGMGEVYLGEDTHIRRQVAIKVTRA
                     EATHYPNSEAARETTRLFQREVRAIAMLDHPYILPLFDYGEQDMEATSLTYMVMPYRR
                     EGSLSTWLRQRGERGGGQTLAPQEVAHFVQQAALALAYSHAHQIVHQDVKPSNFLLRE
                     NELSPGLPDLLLADFGIAKFTSASASMSQSSRGTPTYMAPEQWSGKPVPASDQYALAI
                     MTYQLLTGSVPFQGRQEQVMYQHFNAQPQPPSARNPHIPKEVDTVILRALSKKPEERF
                     PAIAAFSSALKQAMGLQEAKVAPSKASGELPKPTNPLPTGEGDIRATVAISRQEAMNG
                     TIRNLNLPGGRRMAVPIPSGVPDGQVSRLENQGETTGVGTTGALVLTIAVAPDETRMA
                     AQSGNENVTVRSDPVVAKRQVVGEQPSRARRMRFAPRGKAAIIVGLVVLIVLVSSGVL
                     YFSNSNPRRQSLLNAGIADPYMSSFSKIVMNDPLSDNSLGYSWPEGSDSSGD"
     gene            complement(2009..2569)
                     /locus_tag="VGT44_08480"
     CDS             complement(2009..2569)
                     /locus_tag="VGT44_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2580874.1"
                     /translation="MMVDNDNAQDDDGTNRRDFLTTNEAAKQLGVSYYTVYEYIREKR
                     LVAEMVSGVYIIPKKAVEEFKAKPTGRTRVKPPSWRVYRAGARVRSLDITVQARAGQL
                     ERLREKLSRLLDSQAFLFHGTMQRYIFEDETNPSKVTISLIWKDTELPDESSLDQELA
                     DFKVAFADVLDWTTARYTRKRAMIHT"
     gene            2709..3530
                     /locus_tag="VGT44_08485"
     CDS             2709..3530
                     /locus_tag="VGT44_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2580875.1"
                     /translation="MNNAPLSESEHLLLLHRHLSECMGGILPAALDIARVKRVLDVEC
                     GAGGWVYDLAWRYPSLYVVGTESRPHLAREAHALVSRAGNAEVIEQDIHSLSDQAVPP
                     ASFDLVHARFLVSKLTPMEYPAILESLTRRCRTGGMFVWEEPEYPLTNSPACEQFYRL
                     IQDALQAAGRAFSPGPSLGITAQMSNWHKASGCHIMLDTAYALDISATMKGHAAFQQQ
                     LELMSKQLRPFLLRTEVTTEADFDKLYEQAQQEIAGVGFRGVVYVRTMVGTTERA"
BASE COUNT          771 a         1080 c          974 g          791 t
ORIGIN      
        1 ctatcaccgc tagaatcaga accttcaggc caagaatagc ccagactatt atcactcaac
       61 gggtcattca tcacaatctt gctaaagcta gacatatacg gatcagcaat tcccgcattc
      121 agtaaacttt gccgacgggg gttagaattg ctaaaataca acacaccaga gctaaccagc
      181 acgatcagca ccaccaaccc tacaataata gcagccttcc cacgcggcgc aaaccgcatt
      241 cttctcgccc tcgatggctg ctccccaacc acctgcctct tcgccaccac cgggtcagac
      301 cgcaccgtca cattctcatt tccgctctgc gccgccatcc tcgtttcatc cggcgcaacc
      361 gcaatcgtca gcaccagcgc ccccgttgtg ccaacgccgg tcgtctcgcc ctggttctcc
      421 aggcgactca cctgcccatc tggaacgccg gaggggatag gcacggccat gcggcggcct
      481 cccggcaggt tcaggttgcg gattgtgcca ttcatcgctt cctgcctgct gatcgcgacg
      541 gtcgcgcgaa tatcgccctc gccggtgggc aaagggttcg ttggtttcgg caactcgcca
      601 cttgccttcg atggagcgac tttagcttcc tgcaaaccca tcgcctgctt caaagcgctg
      661 gagaaggcgg cgatggcggg gaagcgctcc tccggcttct tcgagagggc gcgcaggatc
      721 acggtgtcta cctcttttgg aatgtgtgga ttgcgcgcgc tgggcggctg cggctgcgcg
      781 ttgaagtgct ggtacatgac ctgttcctgg cggccctgga agggtacgct gccagtaagt
      841 agctggtagg tcataatcgc gagggcgtat tggtcgctgg ctggtacggg cttgccgctc
      901 cactgttcgg gagccatgta agttggcgtc ccgcgcgagg actggctcat gctggcgctg
      961 gccgaggtaa atttggcgat gccgaaatcg gccagcagca ggtcgggcag gccaggggat
     1021 agctcattct cgcgcagcag gaaattcgag ggcttgacat cctggtggac gatctggtgc
     1081 gcgtgggagt aggccagcgc cagggctgcc tgctgcacga aatgcgccac ctcctgcggt
     1141 gcgagcgtct gcccgccgcc acgctccccg cgctgccgca gccaggtcga aagcgatccc
     1201 tccctgcgat aaggcatcac catgtaggtc aggctggtcg cctccatatc ctgctcgcca
     1261 tagtcgaaga gcggcaggat atagggatgg tcgagcatgg cgatggcgcg cacctcgcgt
     1321 tgaaacagcc gtgtcgtctc cctggccgcc tcgctattgg ggtaatgagt agcctccgcg
     1381 cgcgtcacct tgatggcaac ctggcggcga atatgcgtat cttccccaag atacacctcg
     1441 cccatgccac cggttcccag caactgtgcc agccggtatc gcccaagttg catcccctcg
     1501 agaagcatag cgttctacct taaatcaaaa tatcatgtga cgattgggta tgtgatgatg
     1561 gacgtgagga tagtatatca tatggatggc tgagcgaaaa gattgtctct gcgtggcgtc
     1621 atgcaattag atgtgggaca ttctatgcag ctttgtagcg ccgtacatca acctgtacgc
     1681 cttcacttac cgccgtctct gcaatctgag acagtcgctt gcctgtggca gcatcgacat
     1741 attcctcacc acaggtagtg cagatttgag ccggtacatc tcggataaca atggttgtat
     1801 catctctagt caacgtgacc gtcgtagtac cctgttcggt ttctccaaac tggcatatga
     1861 cgcacttcat aaatataccc atgctcttac tcaacgcatt ttcatgtact ataccataga
     1921 aaaagaccgt aatatagagg tgaaacctgt ttctcagagt atgtgaatta atgctggagt
     1981 gtccgagggc gaccgcaagg gtgcgctatc acgtatgtat catcgcccgt ttccgcgtgt
     2041 agcgcgccgt cgtccagtcc agcacatcgg cgaaggcgac tttgaagtcg gccagttctt
     2101 gatccagcga ggactcgtcg ggcagctcgg tatctttcca gatcaatgag atggttactt
     2161 ttgaagggtt ggtctcgtcc tcgaagatgt agcgctgcat ggtgccatga aagaggaatg
     2221 cctgcgagtc cagcagacgc gagagctttt cccgaagccg ctccaactga ccggcgcgcg
     2281 cctgcacagt aatatccagg ctgcgcaccc gcgcgcctgc gcggtagaca cgccaggatg
     2341 gaggtttgac gcgcgtgcgc ccggtcggtt tagccttgaa ctcctcgacg gccttcttgg
     2401 ggataatata gacgccgctc accatttccg ccaccaggcg tttctcgcga atgtattcat
     2461 agacggtata gtacgagacc ccaagctgct tcgccgcctc gttggtcgtc agaaaatcgc
     2521 gccggttggt gccatcgtca tcttgagcat tatcgttatc aaccatcatg ggtatagtat
     2581 aggtatctct gtctttttat gtaaacttca catagctctg tgcttttatt gtaaaaagca
     2641 catcatacct gctataatca tgctatcggc aaatctatat aaaagcacaa tgataaggac
     2701 aatccgttat gaataatgcc ccattgtcag aatcggaaca cctgctccta cttcatcgtc
     2761 acctcagcga atgcatgggc ggcatcttgc ccgctgcgct ggacatcgcg cgggtgaagc
     2821 gtgtactgga tgtcgagtgc ggcgcgggcg gctgggtcta cgacctggca tggcgctacc
     2881 cttctctcta tgtggtagga accgaatcgc gcccgcatct tgcgagagag gcacacgcgc
     2941 tcgtcagccg ggccggaaac gcggaagtga tcgaacagga tatccactcg ctctcagatc
     3001 aagccgttcc gcctgcatcc ttcgacctgg tacacgcgcg cttcctggtg agcaagctaa
     3061 caccaatgga gtatcccgcc atccttgaat ctctgacgcg ccgttgtcgc accggcggca
     3121 tgttcgtctg ggaagagcct gaatacccgc tcaccaacag tcccgcctgc gagcagttct
     3181 accgcctcat acaagacgcg ctgcaagccg ccggacgcgc cttttcgccc ggtccatccc
     3241 ttggcatcac cgcccagatg agcaactggc acaaagccag cggctgccac atcatgctcg
     3301 ataccgccta cgccctggac atctccgcca caatgaaagg ccacgccgcc ttccaacaac
     3361 aattggagct tatgagcaaa cagctacgcc cctttctcct gcgcacagaa gtcaccaccg
     3421 aagcagattt cgataaattg tatgaacagg cccaacagga gatagcaggc gtaggatttc
     3481 gcggtgtagt gtatgtgcgc acgatggtag ggacaaccga acgcgcatga taccgttatc
     3541 aaaccgcgac gaggagccag ggcgatggca tgtcgcatgg atgccagggc gatggccagc
     3601 atcgccccta ccctcg
//
LOCUS       DASZAK010000486         4221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_509544, whole genome shotgun sequence.
ACCESSION   DASZAK010000486 DASZAK010000000
VERSION     DASZAK010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509544"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1282
                     /locus_tag="VGT44_08490"
     CDS             <1..1282
                     /locus_tag="VGT44_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2580876.1"
                     /translation="LQGMGPDGEDVANPVIANGIAYLQEGASNVIAVQDATGRVLWTH
                     EYNSIPSSGPNGVTFANGRIYGVTACGVFALDAQTGRQVWYDTHLATGTVRFDIAPQV
                     ANGKVFVSSAITFGGGIIYALDAATGTTLWSFQTVVDKVGQQLKATAGGAWDAMLIGP
                     DNSVYAGIGNPYLSLQEAQATPSRELYTDSLVKLNQTTGQLEWYYQAFPNDFHDWDLQ
                     ISPIYTTVAGRPVVLAAGKGGVVFAFDATSGQLLWKTSVGIHNGHDDDDQLALDGTLH
                     LQAPYTLEPGEVGGVETNMAAADGVVYVPVDNLPETFATATSRVGTSNFFQGKGEMVA
                     IDIATGKPLWATQLPQLPLGGATVSNDLVFTATFTGELMALSRQDGSVVWTAQLPAGS
                     NATLAIVGNTLLAGAGLPLTTTQHPAVVAYRLGT"
     gene            1335..2282
                     /locus_tag="VGT44_08495"
     CDS             1335..2282
                     /locus_tag="VGT44_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580877.1"
                     /translation="MNAIETNQLTRAFGSLVAVDDLTLAIPEGTVFGFLGPNGAGKTT
                     TVRLLAALIAPTRGSAVVAGHRLGEHNQAIRQAVGILTETPGLYDRLSAWQNLLFFAE
                     MYDLPAGRARGQVERYLRLLDLWERRDDKVGGFSKGMRQKLAIARALLHEPQIIFLDE
                     PTAGLDPEAARVVRDFIKALRTEGRTIFLTTHNLPEADELCDLIGVFRTQLLRLGTPA
                     QLRAGMFGSGTQVQVVGEAAHWLETVRTLSFVQDATASQSTLSVSLAHPDEQNPALVR
                     ALVEAGAPIRAVEPTSHSLEEVYLELVESERTAAAVATK"
     gene            2300..3064
                     /locus_tag="VGT44_08500"
     CDS             2300..3064
                     /locus_tag="VGT44_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2580878.1"
                     /translation="MHKVLVILRKEFLEIVQQRVLLLSILLPSLLFVVIPLIFLQRVG
                     NGSKASSLNIPSLQGLTLHEYTQGVVGTTFSNIFLLLSMIVPSTIAAYSIIGEKTSLT
                     LEPLLATPVRRWQLLAGKMLAALLPAVLLTWVSGSLFIAELAILTDANVVSHVVTGGW
                     LILFLAGTPLLGLIAVAVMTAISSRVNDTRTAQQLSIWAVVPIAGIILGELSGQFELT
                     VLVAVIATVVLVPLAILLTWGAAQLFQRETILTRWK"
     gene            3217..3828
                     /locus_tag="VGT44_08505"
     CDS             3217..3828
                     /locus_tag="VGT44_08505"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2580879.1"
                     /translation="MMQPMKHQRVDPVSSLDQATWQQILTAAKALFFAKGYKGVSMKE
                     IADAVQVMPSALYYHFPKGKEDLFTKMIQTLFMDEGVAGIDQAIGTTQGLRERLTLLT
                     TSLLTLPIDEHFMMLLRDAREHIKDPENQRIILSLRDRIKLQAMGLFQAAQDAGEIRA
                     DIPIEELVSLYMGMLRGSKSIQVSRLVTVLLDGIASPAQRDQH"
     gene            3915..>4221
                     /locus_tag="VGT44_08510"
     CDS             3915..>4221
                     /locus_tag="VGT44_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2580880.1"
                     /translation="MTPSPDPSQTELAADNAPGGTLAIETHSLSKYFGQRKAVDGLTI
                     SIPAGAIAGFVGPNGAGKTTTIRLLLGLVRPSAGSATILGQRLTHPERYLPHVGALVE
                     "
BASE COUNT          836 a         1245 c         1266 g          874 t
ORIGIN      
        1 actgcaaggc atgggtcctg atggtgaaga cgtggccaat ccggtgattg ccaacggtat
       61 cgcctatctt caggaggggg cttcgaatgt gatcgctgtg caggatgcga ctggccgggt
      121 gctctggaca cacgagtaca actctattcc gagttctggg ccaaacggcg tgaccttcgc
      181 caatggcagg atctacggcg tcaccgcttg tggggtgttc gcccttgacg cgcaaacagg
      241 ccgacaagtg tggtatgata cgcatctcgc gacaggcaca gtcagattcg acatcgcccc
      301 acaggtggcc aacgggaagg tctttgtctc gtcggcaatc acctttggtg gcggcatcat
      361 ctatgccctg gacgccgcca ccggcaccac gctctggagc ttccagacgg tggtggataa
      421 ggtgggtcaa cagctcaaag cgaccgcagg gggtgcctgg gatgctatgc tgatcggccc
      481 ggataactct gtctacgcgg gcatcggcaa tccctatctc tcgctccagg aggcgcaggc
      541 gacccccagc cgcgaattgt acacggatag tctcgtcaag ctcaatcaaa cgacgggtca
      601 gctagaatgg tattaccagg ccttccccaa cgattttcat gattgggatc tccagatctc
      661 gccgatctat accacggttg ccgggcgccc ggtggtactc gccgccggca aagggggggt
      721 tgtgttcgcc ttcgacgcca cgagcggcca attgctgtgg aagacatcag tcggtatcca
      781 taacggacac gacgatgatg accagttggc cctcgacggc acactccact tacaggcccc
      841 gtacacgctg gaaccaggag aagtcggcgg ggtggaaacg aacatggcgg ctgcagacgg
      901 cgtggtgtat gtgccggttg ataacctgcc agaaacgttc gcgactgcca catcccgtgt
      961 tggcacgtca aacttcttcc agggcaaagg tgagatggtt gccatcgaca tcgccacagg
     1021 aaagcccctg tgggcaaccc aactgccaca attgccgttg ggaggggcga cggtttccaa
     1081 tgatctcgtg ttcacagcca cgttcaccgg cgagctcatg gcgctctcgc gccaggatgg
     1141 gtcggtcgtc tggacggcgc agctcccggc cggctcgaat gcgacgttgg ccatcgttgg
     1201 gaatacactg ctcgctgggg caggcctccc actgacgacg acgcagcacc cagcggtggt
     1261 cgcctatcga cttggcacgt agggcgtgag gctcccttgc ggcctgcctt tttgaagtag
     1321 tgaaggatga accgatgaat gctatcgaaa ccaaccaatt gacccgtgcc ttcggcagcc
     1381 tggtggcagt ggacgacttg acgcttgcca ttccagaggg aaccgtcttt gggttcctgg
     1441 gacctaatgg cgcgggcaaa acgaccaccg tgcgcctgct cgcggccttg attgccccta
     1501 cgcgcggcag cgctgtggtg gcgggacatc gcctgggcga gcacaaccag gccattcggc
     1561 aagccgtcgg catcctcacc gagacgccgg gcttgtatga ccgcctcagc gcctggcaaa
     1621 acctgctgtt ttttgcggag atgtatgatc tgcccgccgg gcgcgccaga ggccaggtcg
     1681 aacgctatct gcgtctgctc gacctctggg agcggcgcga cgacaaggtt ggtggcttct
     1741 ccaaaggcat gcggcagaaa ctggcgattg cccgcgcttt gctgcacgaa ccacagatca
     1801 tcttcctgga tgagcccacg gctggcctcg atccggaagc cgcgcgcgtg gtgcgcgact
     1861 tcatcaaggc attgcggact gaagggcgga ccatctttct caccacgcat aatctgcccg
     1921 aggccgatga actctgcgat ctgattgggg tgtttcgcac gcagctgctg cgcctgggca
     1981 ccccagcgca gttacgtgct ggcatgtttg gatcgggtac ccaggtgcag gtcgtcggag
     2041 aggcagcgca ctggctcgag accgttcgta ccctgtcctt tgtgcaggat gcgaccgcct
     2101 cccagagtac cctctccgtc agtctggcgc atcccgatga gcagaatccg gcgttggtgc
     2161 gcgccctcgt ggaggcaggt gcccccatcc gcgcggtgga accgacctcc cattccctcg
     2221 aagaggtgta cctggaactg gtggagagtg agcgcacagc ggcggctgtc gccacgaagt
     2281 aagaaggagc catgcaagta tgcacaaagt cctggtcatc ctgcgcaaag agtttctgga
     2341 gatcgttcag cagcgtgttc tcctgctcag tattctcctg ccgtcgctgc tgttcgtcgt
     2401 gattcccctc attttcctgc aacgagtcgg caatgggtcg aaagccagtt cgctgaacat
     2461 cccgtccctc caagggttga ccctgcacga gtacacgcag ggagtggtcg gaacgacgtt
     2521 ctcgaacatc tttctcctgc tgtcgatgat cgtgcccagt acgattgcgg cttatagtat
     2581 cattggcgag aagaccagtc tcacgctgga accgctgttg gcaacgcccg tgcgccggtg
     2641 gcaattgttg gcgggcaaaa tgctggcggc gttgctgccg gcggtgctgc tcacctgggt
     2701 gagtgggagc ctgttcatcg cggaattagc gattttgacc gatgcgaatg tcgtctccca
     2761 cgtggtcacc gggggctggc tcatcctctt cctcgccggc acaccgctct tagggctgat
     2821 cgccgtggcc gtcatgaccg ccatttcgtc gcgggtcaac gatacccgca cggcgcaaca
     2881 actctcaatc tgggcggtgg tgcccatcgc ggggatcatt ctcggcgaac tctccggcca
     2941 gtttgaactc accgtgctcg tggcggtcat tgccaccgtg gtcctggtcc cgctcgccat
     3001 tctcttgacc tggggcgctg cccaactgtt tcagcgcgaa accattctga cccgctggaa
     3061 gtaggagggt aggacgcatc gtatagagag catcatgcag gaggaacaag cctcgtgcct
     3121 cttgagattc cttcttgaca ggatttccct ctctgtgtta ttcttcttac gagcaactta
     3181 atgaacgttc tgtaaattgc aaggagagga taacacatga tgcagcccat gaagcaccaa
     3241 cgagtggacc cggtttcctc cctcgaccag gccacctggc agcagattct caccgccgcg
     3301 aaggctctct tttttgcgaa gggctacaaa ggcgtctcca tgaaagagat cgccgacgcg
     3361 gtgcaggtga tgccctcggc tttgtactac catttcccca agggtaagga agatctgttc
     3421 acgaagatga tccagacctt gtttatggac gaaggggtcg cagggatcga ccaggccata
     3481 ggaaccacgc agggtctgcg agagcgtttg actctgctca ccacctcgct gctgacgctt
     3541 cccattgatg agcactttat gatgctgctg cgagatgcca gggagcatat caaggacccg
     3601 gaaaaccagc ggatcatctt gtccctacga gaccggatca agctacaggc catgggcctc
     3661 tttcaagcag cgcaagatgc cggagagatt cgcgcggata ttccgatcga ggagctggtc
     3721 agtctctata tggggatgct gaggggatcg aagagcattc aggtgtcgcg cctggtcacc
     3781 gtgctgctcg acggcattgc gtctcccgca caacgagatc agcactaaca ctttggtaag
     3841 aaaggaaaga tttcacgcca tgaacagatg gatggtagtc ctctttgata agaggcacgc
     3901 ccacggaggt gctcatgact ccctcccctg atccctcgca gacggaactg gcagcggata
     3961 acgcaccagg cggcactctg gcgatcgaaa cccatagctt gagtaagtac tttgggcagc
     4021 gcaaagccgt cgatggcctc accatcagca tccctgccgg ggccatcgcg ggctttgttg
     4081 gccccaatgg ggccgggaag acgaccacca ttcgcctgct gctgggacta gtgcgaccga
     4141 gtgctggcag cgcgacgatt ctgggtcaac ggttgacaca tccagagcgt taccttccac
     4201 atgttggggc gctcgtcgaa g
//
LOCUS       DASZAK010000487         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_367662, whole genome shotgun sequence.
ACCESSION   DASZAK010000487 DASZAK010000000
VERSION     DASZAK010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367662"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VGT44_08515"
     CDS             <1..937
                     /locus_tag="VGT44_08515"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2580881.1"
                     /translation="LREGRTWLEEMLARTASTERSAARGRALLGAGMLAWAEGDYEAA
                     SPRAEESLSIVREAGDKRLTGWAEAVLGVVRLGQGNTVAARPLLEESRTLFKELGDVW
                     NEAFTLYALGSVVYGSGDPAAARAFYEESLRLFQEQGNVHCSWYASLALSALQVVVST
                     QGDQEMARLLDQQLQPLMQQARNRGALVLFLINIGDIWLHRWGDKQQAKVLYRESLSL
                     WRNLQRVEQGIGIVKALAGLAEVAAAQGRAERAGRLFGAADSLLPSDSVYREDVNRHV
                     AEARANLDAATFEAGWSAGQTMTEEQAITEALQDA"
     gene            997..1233
                     /locus_tag="VGT44_08520"
     CDS             997..1233
                     /locus_tag="VGT44_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580882.1"
                     /translation="MTTQESTPTYARDIQPLFRASDRASMSWAFDLWNYHDVSTHAQA
                     ILERLSSGTMPCDGKWPEEQIAQFRRWVEAGMPA"
     gene            complement(1286..>2365)
                     /locus_tag="VGT44_08525"
     CDS             complement(1286..>2365)
                     /locus_tag="VGT44_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEV2580883.1"
                     /translation="ALVSNLLTVVGSHPYFSHPNFPQRAKYYPPGVQLALLPFGEHAL
                     QHFLYLERPEGMDLEDAPEFAALAIPKPSLTLDDDQIVPQPQEFATIGHLYRGIEQGF
                     RHLVEKYGEERVFIGPPRAQATEEYFGWPELIAVTDLASACQAIETIIEEGEGARGDW
                     RQAHFGKFLQIMQAYRDLQQQDPGFEPARPVVAAYVRPPSDTNEVPLISDPVTAGVSE
                     LFNASYEVVLQMLMRYFIHGTETEDELQTLSGMAVSAMFMVIKPLGQLLTTLPIGPDH
                     PGKVAGPTFEIYQTAYFLSHHDAAWIVLHERLLELAAYCGKLSDQQSAPQADLQAVGE
                     NFRRLAAVLEPHVKTSQAGTHEQPS"
BASE COUNT          466 a          656 c          782 g          461 t
ORIGIN      
        1 tctgcgcgag ggaagaacgt ggttggagga aatgctggcg cggaccgcga gcaccgagcg
       61 gagcgctgca cgaggccggg ccctccttgg tgcggggatg ctggcctggg ccgaaggcga
      121 ctacgaagcc gcctcccctc gtgcagagga gagcttgtcc attgtgcgcg aggcgggaga
      181 caagcgattg accggctggg cggaagcggt gttgggagtt gttcgattgg gtcagggaaa
      241 cacggtggcg gcgcgccctc tgctcgaaga gagccgcacc ctgttcaagg agctggggga
      301 tgtctggaac gaagcgttca cactctacgc tctgggcagt gtcgtctatg gctcaggcga
      361 tcctgctgcg gcgcgtgcat tctacgagga aagtttgcgg ctcttccaag agcaagggaa
      421 tgtgcattgc tcttggtacg cttcgttggc gttgagtgcg ttgcaggtcg tggtctcaac
      481 ccagggcgac caggagatgg cgcgcctcct ggatcagcag ctccagccac tcatgcagca
      541 ggccaggaat cggggggcgc tcgtgctgtt cttgatcaac atcggggata tctggctcca
      601 tcgttgggga gacaagcaac aggcaaaggt gctctacagg gagagcctga gcctgtggcg
      661 gaacctgcaa cgtgtggagc aagggatcgg catcgtcaag gcgcttgctg gactggccga
      721 ggtggcagcg gcccaggggc gagcagagcg agcggggcgg ctgtttggtg ccgccgacag
      781 cttgctacca tccgacagcg tgtaccgtga ggacgtgaac agacatgtcg ctgaagctcg
      841 tgcgaatctc gatgcagcga cattcgaggc cggctggagc gctgggcaga cgatgacgga
      901 ggagcaagcc attactgagg cgttacaaga cgcctagctg aggcaatggc ctcgctctca
      961 cgcagtagtt catacagtcg ggaaaggagc gcgatcatga caactcagga gagcacaccc
     1021 acttatgcac gagacatcca gccgttgttt cgtgccagcg accgagcctc aatgagttgg
     1081 gccttcgacc tctggaacta ccacgatgtc agtacgcatg ctcaggccat tcttgagcgc
     1141 ctctcaagcg ggacgatgcc gtgcgacgga aagtggcccg aggagcagat cgctcaattt
     1201 cgcaggtggg tcgaagccgg catgcctgcg tagtgccctt caggctcacc ccaattgcaa
     1261 tttgcagcac ggactcacgc gactgctagg atggctgctc gtgcgtcccg gcctgactcg
     1321 ttttgacgtg tggttccagc accgccgcca gcctgcgaaa gttttccccg acggcctgta
     1381 agtctgcttg tggtgccgac tgctggtcgc tcaacttacc acaataggct gccagctcaa
     1441 gcagtcgctc gtgcaagacg atccaggccg catcatgatg tgacaggaaa taggctgttt
     1501 gatagatctc gaaggtcggc cccgccacct tgccagggtg gtctggaccg atcggcaggg
     1561 tggtgagcag ttggccgagt ggtttgatca ccatgaacat ggcactcacc gccatgccgc
     1621 tgagcgtctg caactcgtct tccgtctctg tgccatgaat aaaatagcgc atgagcatct
     1681 gcagcacgac ttcgtaactg gcgttgaaga gttcggagac accggcagtg actggatcgg
     1741 agatgagggg aacctcgttc gtatcgcttg gcggtcgcac gtaagccgcg actaccggtc
     1801 gcgccggctc aaaccccgga tcctgctgct gcaagtcgcg gtaggcttgc atgatctgca
     1861 ggaatttgcc gaagtgcgcc tggcgccagt caccgcgtgc tccctccccc tcttcgataa
     1921 tcgtctcgat ggcctggcat gccgaagcca ggtcagtgac cgcgatcagt tcgggccagc
     1981 cgaagtactc ctcggtcgct tgtgctcggg gaggaccgat gaagacgcgt tcttcgccgt
     2041 acttctctac caggtgacgg aacccttgct caatgccacg gtagaggtgg ccgatcgtgg
     2101 caaactcctg gggctgggga acaatctggt catcgtcgag ggtgagactg ggtttgggaa
     2161 tggccagcgc tgcaaattcc ggggcatctt ccagatccat gccttcgggc cgctccaggt
     2221 aaagaaaatg ctgcagggca tgttcgccaa aaggcagcag ggccagctgg acccctggcg
     2281 gatagtactt cgcgcgttgg ggaaagttgg gatggctgaa gtagggatgg gacccaacga
     2341 cggtcagcag gttgctcacc agggc
//
LOCUS       DASZAK010000488         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_188337, whole genome shotgun sequence.
ACCESSION   DASZAK010000488 DASZAK010000000
VERSION     DASZAK010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188337"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(626..1609)
                     /locus_tag="VGT44_08530"
                     /pseudo
     CDS             complement(626..1609)
                     /locus_tag="VGT44_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003147159.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          385 a          645 c          673 g          449 t
ORIGIN      
        1 ccgctccgcc aacgagtcac tgcgaaccga taccggtagt ggcgtcattt cgtgggctca
       61 acgaaaaccg ggttatcgga aaggtccacg cgcgcacgcc tgaccatgct gtaccggcag
      121 ttcgaattca gttcgctccg ccatatatgg acccaaccga gatgcaagta cctgtctgag
      181 tcatgattgg ttgatccggt tgcgaccata tattcggctt ctatgatggg cgtcagagcg
      241 aagcgcccga gccatgatga ggagccgcgc acctggagct ttatcaacga gccggcctct
      301 tagaagcagg gccgtggcgg agcacagcgt tgccaggtgc cggttagacc ggagtgccat
      361 caaatcctct gaagtaagtg tctttcgcaa ctcgttgggg tgaagttcac agttcgagcc
      421 atcttcctta agagcatttt tccgtcccgc ccttcaccct ttgagggcgg gacggaaaaa
      481 tggggctttc tgcactggcg gcgctcacgc ggtgcagctt cagtgtcagg ccggcccggc
      541 ggcctggcgc tgaagctgcg ggtgtggcgc cgcgctcggc gaccggggcg tgggccaaat
      601 tcggtcgata ggcttggcca aagcgcagca gcgcccaggc gatgcgcgcg ttcttgttgg
      661 ccagcgccac cgccgccacg ttgtgaccgc gtttggcctg cagccgctgt aaccaaagac
      721 tgcgcgggtc ggtctttctc ctcgcgtgat agagcgtgga gcgcgcgccg tggaccagca
      781 gggtgcgcag gtagcggctg ccgcgcttgg tgatcggcag catcacagtt ttgttcccgg
      841 tattgctgtg acccggcacc aaccccagat aggcgctgag gtggcggccg tttttgaatt
      901 gccggccgtt gccggcggta gcgaccagcg cggtggcggt gagcggaccg atcccttcaa
      961 ccttgagcag cgcctggcag ctggcatcgt gtctggcgat gttcgcgatc cgccggtcgt
     1021 gaacttttac ctgctgttca tgcagcttga gcatgcgcca ttgcccggcg atcagctgct
     1081 tgagcagagg gctcagccgc tgctcgtcgg gctcgagcaa ctgggcgagc gcgcgctgta
     1141 gtgcggcagt gccacgcgcc accgcgatgc cgtattccag caaaagtccg cggatgtgat
     1201 tggccaggag cgtacattgg cggaccgtca cctgccggac tcgatgcagc gactgtagat
     1261 cctgctgcgc gatcgtcttc ggctcgaccc agcgcatact ggggcggctg ctggcttcac
     1321 agatcgccgc cgcatcgtgg gcgttgttct tgttgccgtc gaggtagggc ttgaccttgt
     1381 gggcgggtaa aagccgtacc gtgtggccca acttgctgat ctcgcggtgc cagtagtgag
     1441 cgccgccgca agcttccagc gcgacctcgc aggctgggag ctgcgagaaa tactccagca
     1501 cccgctcgcg tctgagggtc ttgcggattg ccacacgccc gtgccggtcg accccgtgca
     1561 actcgaagat ctgctttgcg atatccaggc cgattcttgt aactttcacg cttggaccct
     1621 cccacagttc tttcgatgac ctgattgcca tcgctggctc actatgaagc cgttgaacac
     1681 gggctgggtc catcccatca acgagtcact gcgaaccgat acccggtagt ggcgtcattt
     1741 cgtgtcggaa ccgcgacggc agaacgccct cacctgctgc cagggttcga ggctggacgt
     1801 tttagattgg tcagcagata cggcgtgagc gatacgcttg caagaaagtt ggtcgaccga
     1861 ttagtcaata tcaacggcta gagcttccgc ctcaaggaca agcgcaagga ccgggcacgc
     1921 gattgacgcg cctccgatcc cacgcggcac tcccgcataa accgacgcgt aagtcgtggt
     1981 gcgctcgtgc gcgcgtctga ttgaagtccg gcactcttta catggcctct gtgaactcga
     2041 atatcgcgtc aaacagcagt gtgtgggtcg ctggcgaata cgctgagact cccgatattt
     2101 tctttggctc aacgtggggc acggcgatag ctcgtgtaca accctgaacc ga
//
LOCUS       DASZAK010000489         6971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_46471, whole genome shotgun sequence.
ACCESSION   DASZAK010000489 DASZAK010000000
VERSION     DASZAK010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46471"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1620
                     /locus_tag="VGT44_08535"
     CDS             73..1620
                     /locus_tag="VGT44_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEV2580884.1"
                     /translation="MALGLFMAILDNTIVSVTLPQMQKAFHTDFETITWVASAYFLAQ
                     AAVIPIVGYLSDRIGSKLVFLTALILFTAGSALCAFAPTKETLIAFRVFQGIGGGALL
                     PTAFAIIFRIFPPTERGLANALLGIPIMMAPAFGPTVGGYLSTSFAWNAIFTINIPIG
                     IVATILAVLVLPGRDAESENQAGPAGRKGFDLPGLILSMVGFSALVYGITEAGSKGWG
                     NSTVLAFIIVGVAVLVTFIVVELRVSDPVLDLRLFANYTFTLSNIVLWVIAGVLFGSL
                     FLLPLFFENIQGNTALTAGELLISQGLAMGVGMALSGTLYNRVGPRILAATGMALLVV
                     GTYSLTRLDANTSGLSLQIWLILRGLGLGFTMPPLQTLALSAVSNRAMAKASSLVTVT
                     RMVASAIGVAGLTTYLTQQGANHGLAIGKTIQAGLATRQFSGVAATCVQAAGPTFNQA
                     ALRACVAQHAATLGLNDTFWVVVIFCAVSIVLALIVGRDPAIRALKQARARGEEMPQE
                     RVPVMSE"
     gene            complement(1654..2355)
                     /locus_tag="VGT44_08540"
     CDS             complement(1654..2355)
                     /locus_tag="VGT44_08540"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEV2580885.1"
                     /translation="MQTNYAMSQNPVTRLLLACGAIGSLLFILVFLIEGATRPGYSAW
                     HNFVSSLSLSDQGWEQIANFLVCGILMLLFAIGLRQVFHTGRSSLWGPTLMGIFGLAL
                     VVAGIFVTDGSLGYPPGAHGGTWHQTLHGTIHGLAGLVTFTSLAVACFVMARRFAGNP
                     NWKGWALYSIVTGVIVEVSFIASTTVSALDQSGILPGSPTGLLQRIGIIAGWSWIALL
                     AIRLLRREMMVETNR"
     gene            complement(2628..3857)
                     /locus_tag="VGT44_08545"
     CDS             complement(2628..3857)
                     /locus_tag="VGT44_08545"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV2580886.1"
                     /translation="MMNILVLNAGSTSQKCHLYQAAGGFADLRGDAPTPLWKAEADWS
                     GQAGATALTIATARGATREETLQTDKRGEVITLMLETMWSGETGVIDNDQAIDIVGHR
                     VVHGGDEFEESVVVTQAVKDAIARMAVFAPEQNPANLEGIEAAERLLPGVSQVAVFDT
                     AFHRTMPPAAVVYPIPYQWYSDKHVRRYGFHGISHSYCAHRAAQLLGKDLSSLRLITC
                     HLGGGCSLAAIENGRSVDTTMGFTPLEGLMMGSRSGSIDPGVLLYLIREHGYSASQLN
                     TLLNESSGLKGIAGTGDMRQVVQRMQAGDQLASLAFQMFAHRLRSAIGAMMASLGGLD
                     ALVFAGGIGEHSAEVRAAACAPFGFLGIQSDERKNAQISNDGDIAASTSAVRVLVVHT
                     QEEWSIAQECWRIARGQ"
     gene            complement(3878..4555)
                     /locus_tag="VGT44_08550"
     CDS             complement(3878..4555)
                     /locus_tag="VGT44_08550"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEV2580887.1"
                     /translation="MKEKQTSSVAQDEHALARQQREELKRELAESYANLPRRRSARSR
                     RITFVILGIIAIFGVTLSLLVFWPSASAGSVGGLPIGTDAPNFHLPVYGGGDIGQTID
                     LLALRGHPVILNFYSESCQPCLSEVPYLQRFFASHVAQHSFTLLGINQSDPQDDIRPF
                     GIQYHVTYPLLFDAGGTINRVYNVTAIPLTYFIDSQGLVRYVVLQPLTPTTMQQGLAA
                     VGLSLLA"
     gene            4732..5505
                     /locus_tag="VGT44_08555"
     CDS             4732..5505
                     /locus_tag="VGT44_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580888.1"
                     /translation="MSSTTLLMICAGVNVVVTGLFAGLVTRQFAKRRRIYQLYWMIAL
                     LMAFIATLSYVCMVLVGPTSSAGVIFFRLYYILGASLAPSWLGLGSIALVTGPKVTRI
                     TFIVLLLLSVITAILIAFNPLDMHNLAQVAGTSGTGIMVLGVWLAFTITLNTLGVLAV
                     VGVALYSGWKLLRRASSAAGFQPGNLLWANVLILVGDLLNAYAGSSARGGLLVNSFWL
                     VMALGWITFYAGVILIGRRRARATTPASQQATQNTVTSV"
     gene            5590..6672
                     /locus_tag="VGT44_08560"
     CDS             5590..6672
                     /locus_tag="VGT44_08560"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2580889.1"
                     /translation="MSDQTGPGKLTRGKPYDSQPPNSSILQDGLNESELLREQAHTYL
                     HSGQWAEAGRAFSGLLDIDLEDEDALEGLGQALEKLDDFQALLDITSEIAEANPGSSL
                     ALALKARALQKLDRLSEATIANDQALLLNTNLALAWFNRSGQQVLAGHYEEALRCAER
                     AIELDPADARVWANKSNALNYLNRFGEALEAINQSIARDPDYLFALLAKGELLRKFGR
                     LEEAEQVARRALEIEPDNGAAFSILAFALRGLGQFEQLLPVTDKLVRISPDYLFGWDS
                     RTSALRGLGRFEEALESIEHVLDTDPTIVRYHMIKADTLYRLGRYREAVNAAELAIQI
                     DDEYPPALRIYEKATRALYQHKRKKK"
     gene            6703..>6971
                     /locus_tag="VGT44_08565"
     CDS             6703..>6971
                     /locus_tag="VGT44_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580890.1"
                     /translation="MRRLQTWPRLLLTLPFAIRLAIVVVTTVLALAFYIISFSTLHNG
                     NVLVIPCVLSAWVFKKRGLLLYLAVGILVGTIYHATRLQTLLWPLP"
BASE COUNT         1483 a         2060 c         1977 g         1451 t
ORIGIN      
        1 tggcatagtc aacccatccc tggaaaggtc tgaaaagtga caaaaggata ccgatggcgg
       61 atagcggctg tcgtggcgtt gggcttgttt atggctattc tggataatac cattgtcagt
      121 gtaacgctgc cacagatgca aaaagccttc catacggatt ttgaaactat aacgtgggta
      181 gcctccgcct acttcctggc gcaggccgcc gtcatcccca ttgtcggcta tctcagcgat
      241 cggatcggct caaagctggt ctttctaacg gcgctgatcc tgtttaccgc cggctcggcg
      301 ctgtgcgcgt ttgcgccgac aaaagaaacg ctcatagcct ttcgcgtctt ccagggcatc
      361 ggcggcggcg cgctgctgcc gacagccttc gcgatcattt ttcgcatctt cccgccgacc
      421 gagcgcgggc tggctaacgc gttacttggc attccaataa tgatggcgcc ggcttttggc
      481 ccaacggtcg gcggctacct gagcaccagt ttcgcctgga acgcgatctt taccattaac
      541 ataccaatcg ggatagtggc gacgatcctg gctgtgctgg tgttgccggg acgcgacgcg
      601 gagtctgaga atcaggcggg accggcgggc aggaagggct ttgatttgcc gggccttatt
      661 ctctcgatgg tcggcttcag cgcgctggtc tacggcatta ccgaggccgg ctcgaaaggc
      721 tggggcaact ccaccgtgct ggccttcatt atcgtcggcg tagccgtgtt ggtgaccttt
      781 atcgtggtcg aactgcgcgt gagcgacccg gtgttagatc tgcgcctctt cgcgaattac
      841 acgttcacgc tctcgaatat cgtcttatgg gtcatcgcag gggtgctctt tggcagcctg
      901 ttcttgcttc cgctcttctt tgaaaacatc cagggcaata cggctctgac tgccggtgag
      961 ctgctgatca gccaggggct tgccatgggc gtaggcatgg cgctctccgg caccctgtat
     1021 aaccgggttg gcccgcgcat tctggcggcg accggcatgg ccttgctggt tgtcggcaca
     1081 tacagcctga cccggctgga tgcaaacacc tcaggtctgt cgctgcaaat ctggttgatc
     1141 ctgcgcggcc tgggactggg ctttacgatg ccgccactgc aaactctggc cctgtcggca
     1201 gtgagcaaca gagcaatggc taaggcctcc tcgctggtca cggtcacccg catggtcgct
     1261 agcgccatcg gtgtggcagg tctcaccacc tacctgaccc agcagggcgc aaatcatggc
     1321 ctggctatcg ggaaaacgat tcaggcgggc ctggcaacgc gccagttcag cggggtcgcc
     1381 gcaacctgcg ttcaggcagc cggtcccacg ttcaaccagg ccgccctgcg cgcttgcgta
     1441 gcccagcacg cggcaaccct tggcctcaac gataccttct gggtagtggt gatcttctgc
     1501 gcggtctcaa ttgttctggc cttgatcgtg ggacgcgacc cggcgattcg cgccctcaag
     1561 caggcgagag caagagggga agagatgcca caagaacgcg taccggtgat gagcgagtaa
     1621 gtaaacagag agtttcttgc ataagcccac gaattaccga ttcgtctcca ccatcatctc
     1681 cctgcgcaac aggcgtattg ccaggagcgc gatccagctc cagccagcaa taatcccgat
     1741 gcgttggagc aggccggtcg gcgaccctgg gaggatgcca ctttggtcga gcgcagagac
     1801 agtggtggag gcgatgaagg atacctctac gataacacct gttacaatgg agtagagcgc
     1861 ccagcctttc caattgggat tacccgcgaa gcgccgcgcc atcacaaaac aagctaccgc
     1921 cagggaggtg aaggtgacaa gaccggcgag gccatggatg gttccgtgca gcgtctggtg
     1981 ccaggtgccg ccatgcgcgc ccggcggata gcccaggctg ccatcggtca caaagatacc
     2041 cgcaaccacc agggccagcc cgaaaatgcc cattagcgtg ggtccccaca gcgaacttcg
     2101 acctgtatgg aagacctgcc gcagtccaat tgcgaataaa agcatgagta ttccgcagac
     2161 caggaagttg gcaatttgct cccatccctg atcgctcaag ctgagactgc tcacaaagtt
     2221 gtgccacgcg ctgtagccgg ggcgtgtagc gccctcgatc aaaaatacaa ggatgaagag
     2281 caacgacccg attgcgccac aggcaagcag caaccgggta acggggttct ggctcatcgc
     2341 atagttcgtc tgcatgatac ggtatctcct ttttcgtcgt tcgcgctcat gaactgcagt
     2401 agctgtccca agcatacgcg catcgccagt ttttcacccg cgtctccagt cgtgttgtgg
     2461 ataggactgg agaagggtct gtgtttgggg atgcgcgttg tcgctcgatt cgtcaactgc
     2521 tatgatagag gaaacagttt gcacaatcag gaactgtaac atgcgtgtat gcgctcaaag
     2581 ctctcctctg ctgtaacctc agccgaaagc tgaaaggctg aagcagccta ttgcccgcgc
     2641 gctatccgcc agcattcctg cgcgatgctc cattcctcct gcgtatgaac gaccagcacg
     2701 cgcacagccg acgtggacgc ggcgatatcc ccatcattgg agatttgcgc gtttttgcgc
     2761 tcgtccgact ggatgccgag aaatccgaac ggcgcgcagg cggcggcgcg tacctcagcc
     2821 gaatgctcgc cgatgccccc ggcaaagacc agagcgtcca gcccgcccaa cgaggccatc
     2881 atcgcgccaa tcgctgaacg caggcggtgc gcgaacatct ggaaggccag cgacgccagt
     2941 tgatcgcctg cctgcatacg ctgcacaacc tggcgcatat ctccggtgcc tgcaatgcct
     3001 ttcaagccgg aagattcatt aagcagcgtg ttgagctggc tggcgctata cccatgctcg
     3061 cgtatgaggt agagtaggac gccaggatcg atggagccgg agcgactacc catcatcaat
     3121 ccttcaaggg gagtaaagcc catcgtggta tcaacgctgc gcccattctc gatggcggcc
     3181 agcgaacagc cgccgcccag gtggcaggtg atgagccgca gggaggacag gtccttgccg
     3241 agcagctgcg cggcgcgatg cgcgcaatag ctgtggctga tgccgtgaaa cccatagcgt
     3301 cgcacgtgct tatcgctgta ccattgatac ggaatcgggt aaacaacagc ggccggcggc
     3361 atagtgcggt gaaaggcagt atcgaacact gctacctgag agacaccggg cagcagccgc
     3421 tcggcagctt cgatgccttc caggttcgcc gggttctgct caggagcaaa caccgccatg
     3481 cgtgcgatgg catccttgac cgcctgcgtg accaccacac tctcctcgaa ttcgtcgcct
     3541 ccgtgaacga cgcgatgccc tacgatgtcg atggcctggt cattgtcgat cacaccggtc
     3601 tcgccgctcc acatcgtctc aagcattaga gtaatcacct cgccgcgctt atccgtttga
     3661 agggtctctt cacgtgttgc gccacgcgca gtcgcaattg tgagcgcggt cgcccctgcc
     3721 tggcccgacc aatcagcctc ggctttccac agcggcgttg gagcatcgcc gcgtagatcg
     3781 gcgaagccac cagctgcctg gtagaggtga cacttctggc tggtagagcc agcattcagc
     3841 acaagaatgt tcatcatcaa ctcccttgcc aatttcttta cgctagcaaa gatagaccca
     3901 ccgccgccag gccctgctgc atggtcgtgg gcgtaagcgg ctgcaggaca acatagcgca
     3961 caaggccctg gctatcaatg aagtaggtca gaggaatggc agtcacgttg tagacacgat
     4021 tgatcgtgcc gcccgcgtcg aacagcaggg gataagtgac atgatactgt ataccgaaag
     4081 ggcggatatc atcttgcggg tcggactggt tgatgccaag aagtgtaaac gagtgctgcg
     4141 cgacgtggct ggcaaagaaa cgctggagat agggaacctc cgaaagacac ggctggcacg
     4201 actcggaata gaagttgaga atgacagggt gcccgcgcag cgcgagcaga tcaatggtct
     4261 gcccgatatc tcccccgcca tagacgggca aatgaaagtt gggtgcatcc gtgccgatcg
     4321 gcaacccgcc caccgatccc gcgctcgccg acggccagaa gaccagcaga ctgagcgtca
     4381 ccccaaagat cgcgatgatg ccgaggatca caaaggtaat acgtcggctg cgcgccgagc
     4441 gccggcgtgg aaggttggca tacgactccg ccagctcccg cttgagttct tcgcgctgct
     4501 ggcgcgccag cgcatgttca tcttgagcga cactcgatgt ctgtttttct ttcatgaaaa
     4561 atagtataag cgtcacccgc aacgtgtgca agcgatcaaa caagattcta aaggttttag
     4621 gattgacgaa cccaatcacg aggagtattc ttactgttgg caacatattt ttgtagtgtg
     4681 gccgtgtatc aaacatgcca catcttcata ctgcaagggt ggaattgaat catgtccagt
     4741 actactcttc tcatgatctg cgcaggggtc aatgttgtag taacgggcct gttcgcggga
     4801 ctggtcacgc gccaattcgc gaagcgacgc cgtatctacc agctctactg gatgattgcg
     4861 ctgttaatgg cctttatcgc cacgctgagc tatgtctgta tggtgctggt cggcccaacc
     4921 agcagcgccg gcgtcatctt tttccggctc tactacattc tgggcgcctc gctcgcaccg
     4981 tcctggctcg gtctgggcag catcgcgctc gtgaccgggc caaaagtcac gcgcatcacc
     5041 tttattgtcc tgctcctctt gagcgtaatc acagccatcc tgatcgcctt caatccgctc
     5101 gatatgcata acctggccca ggtcgcgggg acatccggca ccggcatcat ggtactgggc
     5161 gtctggttgg ctttcacgat taccctcaac accctgggcg tcctcgcagt agtcggagtc
     5221 gcgctctatt ccggttggaa gctgctgcgt cgcgcttcga gcgcggcagg atttcagccc
     5281 ggcaacttgc tctgggcaaa tgtactgatt ctcgtaggag acttgctgaa cgcctacgcc
     5341 ggatcatccg ccaggggagg gctactggta aacagcttct ggctggtgat ggccctcggc
     5401 tggatcacat tctacgccgg agtaattctg atcggacggc gtagagctag agcaacaaca
     5461 cccgccagtc agcaagcgac acaaaatacg gtgacatccg tatagagaca ggactatatt
     5521 acctattgct tctattgacc ggttctgcga taatagcggt gacattgtgc tggacggaga
     5581 atatttcacg tgagcgatca aacaggaccg gggaagttaa cgcggggcaa accctacgat
     5641 agccagccac caaatagcag catactccag gacggtttga acgagagcga gctactacgc
     5701 gagcaagccc atacctatct gcacagcggg caatgggctg aagcaggccg cgccttttcc
     5761 ggcttgctcg acatagacct ggaagacgag gatgcgctgg aaggacttgg gcaggcattg
     5821 gagaaacttg acgacttcca ggcattgctg gatatcacct ccgagattgc tgaagcgaat
     5881 ccaggctcgt cgctggcgct ggctctcaag gcccgcgcgc tgcaaaagtt ggaccggctc
     5941 tcagaagcca caatcgccaa cgaccaggcg ctgctgctga ataccaatct cgcgctggcc
     6001 tggttcaatc gcagcgggca gcaggtactc gccggacatt acgaggaagc cctgcgctgc
     6061 gccgaacgcg ccatcgaact cgaccctgcc gacgcgcgcg tctgggccaa caaatccaac
     6121 gcgctgaact acctcaaccg cttcggtgaa gcgctggaag ccattaatca gagcatcgcg
     6181 cgcgaccccg attacctctt cgccctgctc gccaaaggcg aattgctgcg caaatttgga
     6241 cgcctggaag aggccgagca ggttgcccgc cgcgcgctcg aaatcgagcc ggataatggc
     6301 gctgccttca gtatcctcgc ctttgccctg cgcggtctcg gccagtttga acaactactc
     6361 cccgtcacag ataaactggt tcgtatctca cccgactacc tcttcggttg ggactcccgt
     6421 accagtgcgc tgcgcggcct gggacgcttt gaagaagccc ttgagtctat cgaacacgtc
     6481 ctggataccg acccgacgat tgtgcgctac cacatgataa aagctgatac tctctatcgt
     6541 ctgggccgtt atcgggaagc ggttaacgca gctgaactgg ccatccagat cgatgatgaa
     6601 tacccacccg cgctcaggat atacgagaag gccacccgtg cgctgtacca gcacaaacgc
     6661 aaaaagaaat aatcgttagt ggactacggg ggtaatgcct gcatgcgccg actacaaacc
     6721 tggccgcgac tattgctcac tttgccattc gcaatacgcc ttgcgattgt cgtggtaaca
     6781 acggttctgg cgctggcctt ctatatcatc agtttttcta ccttgcacaa tggaaatgta
     6841 cttgtgattc cctgtgtcct gtcagcctgg gtatttaaga aacgaggact gctgctctac
     6901 ctggccgttg gcatattggt agggacaatc taccacgcca cgcggttgca gacgctgctc
     6961 tggccactac c
//
LOCUS       DASZAK010000490         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_580424, whole genome shotgun sequence.
ACCESSION   DASZAK010000490 DASZAK010000000
VERSION     DASZAK010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580424"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VGT44_08570"
     CDS             <1..813
                     /locus_tag="VGT44_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580891.1"
                     /translation="LRLASDTRSAWWIGNNTAYLARAYLLQGALTSAEEALKAVMPRE
                     QPPANTPERRISWAWGELALASDEPEMALGIADRLLASVPGAKRVQPIPWLLKLKGEA
                     LGALGRREEAIQVLEEAAQGTLARQERPLLWQLQRTLGRQYQYLKQEDLAQRSFTSAR
                     QGIASLASTIDDAYLREHFLRTALITLPKEKPVTASRVAKEAFGGLTEREREVAAFIA
                     QGKSNREIADQLVVSYRTVETHVSAILSKLAFSSRAQIAVWAVEIGIVKQAE"
     gene            1330..>1613
                     /locus_tag="VGT44_08575"
     CDS             1330..>1613
                     /locus_tag="VGT44_08575"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2580892.1"
                     /translation="MTDSISIRADRAALAAVFAEPAWLAEHLNDPGVRVVEVDVSQVA
                     YNQGHIPGAVLWNAYTDLHHADYSPVDRSEFAELLKRSGLTPNTTIVFYG"
BASE COUNT          372 a          451 c          459 g          331 t
ORIGIN      
        1 ctgcgactgg catccgacac ccgttcggcc tggtggatcg gcaataatac cgcctatctt
       61 gcgcgagcct atctgctgca aggagcattg acatcggcag aggaggcact caaagcggta
      121 atgcctcgcg aacagccgcc cgcgaacaca ccagaacggc gcataagttg ggcgtggggt
      181 gaactcgccc tggcaagcga tgaaccggag atggctcttg gcatagctga ccgattgctt
      241 gcctcagttc caggagcgaa aagggtacaa cccattccct ggctcttgaa gctcaaaggt
      301 gaggcgcttg gggcgcttgg acgccgggag gaagcgatac aagtgttgga ggaagccgcg
      361 cagggcactc ttgctcgaca ggagcgacca ctcctctggc agctccagcg tacactcgga
      421 cggcagtacc aatatctcaa gcaggaagat ctggcccagc gcagctttac ctcggcgcga
      481 caggggattg catccctggc aagcacaatt gatgatgcct acctgcgcga acacttcctg
      541 cgtaccgcac tcatcacgct tcccaaagaa aaaccggtca ctgcgagccg tgttgccaaa
      601 gaagcctttg gcgggctgac ggagcgcgaa cgcgaggtgg cggccttcat cgcgcagggg
      661 aaatcgaacc gtgagatagc cgaccagctg gttgtcagct accggacagt cgaaacccac
      721 gttagcgcca tcctctccaa actagcattt tcctcgcgtg cgcagatcgc tgtatgggcc
      781 gtcgaaattg ggatcgtgaa acaggccgaa tgagcagaac tgcgtggaaa cgcgcaggga
      841 tagataggga taaggcgcac ttcacacctc ccggtatcac attttctccg tgttcctcct
      901 gcatacatct acgggccgtt tgcgtaaaac atctgtgttt ttcccgatgg aatgttagcg
      961 ctttctgagt acactactga caacgactgg aattaaaggg attccggtcg ctcgacaatc
     1021 gaaactgttt tgcagtagtt cacacaagga ggaacactat ggcgactcag gcggacacga
     1081 agaaaaccta tgttctcgat ggttttctct tggaagcatg ttcatgtatg gcaccctgtc
     1141 cttgttggat cggggacgat cctgacggag ggaagtgcga cgcctcagtc ccgtcatagg
     1201 aatggcgctg ctactgctga cccttctgtg gctggctcat cctacctggc tactgagtat
     1261 tggagtgtaa tagcgactct cgttacgcgg ggaaaaagca gtactcgcat atataaggag
     1321 gaacttaaat tgacagacag catttctatt cgggctgacc gcgcagcgct tgcggccgtg
     1381 ttcgcggaac cagcatggct cgccgagcat ctcaacgatc caggtgttcg cgtggtcgaa
     1441 gtggatgtca gccaggtggc atacaaccag ggccacatcc ccggagccgt cctctggaac
     1501 gcgtacaccg acctgcacca tgctgattac agcccggtgg atcgcagcga attcgcggag
     1561 ctgctcaaaa ggtctggtct cacgcccaac acgacgatcg tcttctacgg cta
//
LOCUS       DASZAK010000491         2093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_616630, whole genome shotgun sequence.
ACCESSION   DASZAK010000491 DASZAK010000000
VERSION     DASZAK010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616630"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGT44_08580"
     CDS             complement(<1..213)
                     /locus_tag="VGT44_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV2580893.1"
                     /translation="MFDILQYTFIQNALLAGSCVAVVAAVAGYFMIVRGLTFAGHALP
                     NIGFAGAAGAVLLGIDPVIGLFVFTIV"
     gene            complement(258..1025)
                     /locus_tag="VGT44_08585"
     CDS             complement(258..1025)
                     /locus_tag="VGT44_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2580894.1"
                     /translation="MKDPVIDLEHVRVQYGRQIILEDISMTVGEGEFIVILGPNGAGK
                     STLLKLLLGLVKPSAGVVRVLGSPPRRGNNAIGYAPQHRVLEADLALRARDVVGFGLD
                     GNRWGPGLPSRKRDAIIDKALLEVDAADFADVPVGQLSGGEQQRLLIAQALLTNPRLL
                     LLDEPLANLDISHEQEIVELVAQVCRARNVAVMLVTHDINPLLTVAGRVLYMAGGRGT
                     IGSPDEVITSATLSQLYGTPVEVVHALGRVFVVGAET"
     gene            complement(1164..>2093)
                     /locus_tag="VGT44_08590"
     CDS             complement(1164..>2093)
                     /locus_tag="VGT44_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEV2580895.1"
                     /translation="EIHMSLLLRRAYPLLILLLFLLSACGDSSTATPGGSSSPTAISV
                     VAAENFYGDITRQLGGSHVSVTSILSDPNVDPHEYESNVQTAETVSKAQLVIENGLGY
                     DTWMDRLLAASPKPGRIVLVAGDIANHKLTDNPHVWYGFDNVTTIAQAITGALKKLDS
                     NDASTFDNNLAAFNQSLMPLEQKISAINSKYAGTPVALTETIYLYQSTPEGLDVLTPF
                     EFEKAIAEGNDPPADTVTITNDQITRKQVKVLIYNVQTITPITTNLQNEARRLNIPIV
                     PVSETMPPAKTYQTWMMDQLDTLEQALATATGK"
BASE COUNT          453 a          608 c          577 g          455 t
ORIGIN      
        1 cacgatagta aaaacaaaca acccgatcac ggggtcaatt cccagcagaa ccgcacccgc
       61 cgctccggcg aagccgatgt ttggcagcgc gtgcccggcg aaggtcaggc cgcgcacaat
      121 cataaagtaa cccgcaacgg cggcaacgac tgccacgcaa gaccccgcga ggagagcatt
      181 ctggatgaat gtatattgta agatatcgaa catagccgtt ttcctcagct tcgcatgctg
      241 gcgatactac cttggtacta cgtttctgca ccgaccacga agacacgccc cagcgcgtgc
      301 accacctcga cgggcgtgcc atacaattgc gaaagcgtgg cgctcgtgat aacctcgtcg
      361 ggtgagccaa tggtgccacg tccgccagcc atgtaaagga cgcgacccgc cactgtaagc
      421 agcggattga tatcatgtgt gaccagcatg acggccacat tgcgggcacg acagacctgg
      481 gcaaccagtt ccacgatctc ttgctcgtgg ctaatgtcga ggtttgccag tggctcatcg
      541 agcaacaaca agcgcggatt ggttaacagc gcctgggcaa tcaataagcg ctgctgctca
      601 ccacccgaca actgccccac ggggacatcg gcgaaatcag ccgcgtccac ctccagcaaa
      661 gccttgtcaa tgatggcatc ccgcttgcga ctgggcagtc ctggccccca gcggttaccg
      721 tccagcccga atccgacgac atcacgtgct cgcaaggcga ggtctgcttc gagcacgcgg
      781 tgctgcggcg cgtagccaat ggcattgttg ccacggcgtg gagggctgcc caacacccgc
      841 actacgcctg cactgggctt aaccaggccg agcaacagct tcagcagggt actctttccc
      901 gcgccattgg gaccaagaat gacgatgaac tctccctcgc ctaccgtcat gctgatatct
      961 tctaaaatga tctgacgccc atattgaacg cgaacgtgct ccaggtcaat gaccgggtcc
     1021 ttcaccaggt tattgctcct tcctttggtc ctgcctgact gacgcagtca gtagacactt
     1081 cctttggtcc tggctgacgg acggagtccg tagacgcgcc agcgtcgttg ccaggaccca
     1141 tttaaaacta cgtccttttc cagctacttg cccgttgctg tcgccaatgc ttgctccagc
     1201 gtgtccagtt gatccatcat ccaggtttga taggtcttgg ccggcggcat ggtctccgag
     1261 accggtacga tcgggatgtt gagcctcctg gcctcgttct gcaggtttgt cgtgatcggc
     1321 gtgatggtct gcacgttgta gatcaagacc ttcacctgtt tcctggtaat ctgatcgttt
     1381 gtaatcgtca ccgtatcagc aggcggatcg ttaccctcgg caatcgcttt ctcaaactcg
     1441 aacggggtta gcacatcgag gccttccggg gtgctctggt agagatagat cgtttctgtc
     1501 agagcaactg gagtgcccgc gtacttcgag ttgatcgcac tgatcttctg ttcaaggggc
     1561 atcaatgatt ggttgaatgc tgccaggtta ttgtcaaagg tcgaggcatc gttactgtcg
     1621 agcttcttca gggcaccggt gatcgcctgg gcaatggtgg tcacattgtc aaagccgtac
     1681 cagacatggg gattgtcggt cagcttgtga ttagcgatat ccccggcgac cagtacgata
     1741 cggccaggct tgggagaagc agcgagcaac cggtccatcc aggtgtcgta gccaagcccg
     1801 ttctcgatca cgagttgcgc cttactgacc gtctcggcgg tttgcacatt ggactcatac
     1861 tcgtggggat caacattggg atcggagaga atgcttgtga cggaaacatg gctgcccccc
     1921 aattgcctgg tgatatcgcc gtagaagttc tccgctgcca cgacgctaat agccgttggg
     1981 gatgaagatc cgccgggggt ggctgtagag ctatccccac aggcgctcaa caaaaagagc
     2041 aagagaatga gcagtggata agctctcctc aacagtagag acatgtggat ttc
//
LOCUS       DASZAK010000492         5183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_154247, whole genome shotgun sequence.
ACCESSION   DASZAK010000492 DASZAK010000000
VERSION     DASZAK010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154247"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VGT44_08595"
     CDS             <1..1075
                     /locus_tag="VGT44_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580896.1"
                     /translation="GVVGLALVVPVIVLSLPAGHIIDRFDRKTIVIVSQLVLALVSLG
                     LTALSLTRGPLLLVYGCLVLYGCAGAFSSPASSTLVPQVVAEDAFESAAKWDSSVGQL
                     ASVLGPALGGLLIALRGSAALVYAVNAGAILVFVCLLCFLRVVRQAEPATEATTLQSL
                     GEGLRFLRKTQVILASITLDMFAVLLGGAVTLLPIYAKDILHVGPVGLGWLRASPSIG
                     ALAFSFIIAYVPPFKRAGRTLLLTVAGFGAATVIFGVSRSFWLSLAMLALVGGFDNIS
                     VVIRSTLLMTRTPNALLGRISAVNFIFIGASNELGGFESGLVAQFFGPVIAVAGGGIG
                     TMLVVLLVALLWPEMGRLGAMTT"
     gene            1192..2697
                     /locus_tag="VGT44_08600"
     CDS             1192..2697
                     /locus_tag="VGT44_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEV2580897.1"
                     /translation="MIRPDATGRTEQPALPPPFSHYTLDAAYDEMLDAEGRPRPQYRE
                     LFHRLLEMPAETLYQRQQAANTAFLNQGITFTVYGNDEGTERIWPYDLLPRIITGEEW
                     EIIEKGLTQRITALNLFLKDIYGDGRILADGTLPRWLVYSCRHYRREMRGVRVPHDIY
                     VAVDGADLVRLPDGRFAVLEDNLRVPSGVSYMLANRQVMKRTFSSLFGSYGVRPVDHY
                     GQALLSTLLSLAPVGRGEPVIVLLTPGVYNSAYFEHAFLARQMGIELVEGRDLIAHDN
                     IVYMRTTGGLRRVDVIYRRIDDDFLDPLAFRPDSSLGVPGLLNAYRAGNVALANSIGT
                     GVADDKAIYAYVPTIIRYYLDQDPILPNVETYLLNDATQRTYVLERLDELVVKAVGES
                     GGYGMLIGPASTEAQREEFRAQIIANPRNFIAQPTLALSRAPCVVDNQIEPRHVDLRP
                     FVLYGEHVTIVPGGLTRVALKRGSLVVNSSQGGGSKDTWVLTGPAPMEGGA"
     gene            2698..3687
                     /locus_tag="VGT44_08605"
     CDS             2698..3687
                     /locus_tag="VGT44_08605"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEV2580898.1"
                     /translation="MLSRVADSLYWMSRYLERAEHTARLVDVQLNQMLDQAGGETNLR
                     WQQLPRSLRMPQPEGEVDAYSVTQMLTFDVGNSLSIVSCIASARENARQVRELISSEM
                     WEQLNRLFLKVKEASMQQIWYGEPHRYLNSVKEGIYLFQGITDATMSHSEGWHFIRVG
                     RFLERATGTAALLDEHFAAFTTPLAAPGEDESEPLDYLSWVGLLKSCASFEAYCTVYT
                     AVIRPANIAEFLLLNAEAPRSIRFSCAMMQGALQAIAKSTGARNPGRVERIIGRLRAA
                     LDYDQIEEIFGGDMHEYLENIRRQCGQIHSGLYQTYIAYPIEVALTSRGAAQE"
     gene            3690..4667
                     /locus_tag="VGT44_08610"
     CDS             3690..4667
                     /locus_tag="VGT44_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEV2580899.1"
                     /translation="MYYTIRHTTRFRYSAPINESIMEVRMQPRSEDGQFCLDFHLHTS
                     PRAQIMTYRGEFGNRVHHFDIPNSHSQLTITAESLVDLTPPAPLPGALDGFAWQELDT
                     LTAKDEYWDTLMPSHYATPSLLLDELAQELEVGRRADPLTTLRELNSALYDAFDWVPT
                     ATQVDSPIDEALRMRKGVCQDFAHIMITLVRQLRIPCRYVSGYLIQHGQLERAERAAN
                     DAMHAWVEAYLPSLGWVGFDPTNDTLADERHVRVAVGRDYADVTPTRGIFRGKATTEL
                     AVTIRYAPSQTALTEDAPPELAWVAATHDEYDREQAPSQVQSQPQQQQQ"
     gene            4802..>5183
                     /locus_tag="VGT44_08615"
     CDS             4802..>5183
                     /locus_tag="VGT44_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580900.1"
                     /translation="MIVLHALWDHRAADATGRLHIWAESSQMPLPVGAGRKARGRQPQ
                     KEEKPREHPFALHYELLRQSLGELVGRLHISGGEASTLDVRLPSTDKGPLASPELILD
                     EVEALQATAFLPYTVPTLALTPDFA"
BASE COUNT          937 a         1523 c         1612 g         1111 t
ORIGIN      
        1 gggggttgtc gggttggcgc tggtcgtgcc tgtgatcgtg ctgtcgttac cggcggggca
       61 tattattgac cgtttcgacc gcaagacgat tgtcatagtc tcccagcttg tattggcgct
      121 ggtttcgctg ggactgacgg cgctgtcgct gacacgcggg ccgctgctcc tggtctacgg
      181 ctgcctggtg ctatatggct gcgcgggggc tttcagtagc ccggccagtt cgacgctggt
      241 tccccaggtg gtcgcggagg atgcctttga gagcgcggcg aagtgggata gcagcgtggg
      301 gcaactggcc tcggtgcttg gtccagcgtt gggcggcctg ctgatcgcgc tgcgaggaag
      361 cgcggcgctg gtgtatgccg tgaacgccgg cgctattctg gtctttgtct gcctgctgtg
      421 cttcctgcga gtcgtccggc aggctgaacc tgccactgaa gcaactacct tgcaatcgct
      481 gggcgaaggc ttgcggtttt tgcgcaagac gcaggtgatc ctggcctcca tcacgctcga
      541 tatgttcgcg gtgctgctgg gcggcgcggt gacgctgctg ccgatctacg cgaaagacat
      601 cctgcatgtc ggaccggttg gattgggctg gctgcgcgct tcgccctcta tcggcgcgtt
      661 agcgttttca ttcataatcg cctatgttcc gccgttcaag agggccgggc gcacgctgct
      721 gctgacggtg gccggttttg gcgcggcgac cgtcatcttc ggcgtttcgc gctcgttctg
      781 gctctcgctg gctatgctgg cgctggtcgg cggattcgac aacatcagcg tcgtcattcg
      841 ttccacgctg ctgatgacgc gcacgcccaa tgccctgctc ggtcgcattt cggcggtgaa
      901 tttcatcttc atcggcgctt ccaacgagct tggcggtttc gagtctggcc tggtggcgca
      961 gttcttcggt ccggtgatag ccgtcgcggg aggcggcatc ggcacaatgc tggtcgtgct
     1021 gctggttgct ctgctctggc cggagatggg gcgattgggc gcaatgacaa cttgacaatt
     1081 tcattcatgg ataggtacta ttagcataga ctgatagctt gtccaacgaa gaatccccgg
     1141 cgctgctgcc ccttgttttt ggtttggaag catagtgtag ggaaattgcc tgtgattaga
     1201 ccggatgcca ctggacgtac tgagcagcca gcgctgccgc cgcctttctc gcattacacg
     1261 cttgacgcgg cttatgatga gatgcttgat gccgagggca ggccgcgtcc acagtaccgg
     1321 gaattatttc accgcctgct ggaaatgcct gccgagacgc tctaccagcg ccagcaggcg
     1381 gccaataccg cctttttgaa ccagggcatc accttcacgg tctatggcaa cgacgaggga
     1441 accgagcgta tctggccgta tgacttgctg cctcgcatta tcaccggcga ggagtgggag
     1501 attatcgaaa agggcttgac gcagcgtatt accgctctca acctgttcct caaagatatc
     1561 tacggcgatg ggcgtatcct ggccgacggc acactgccgc gctggctggt gtatagctgc
     1621 cgccattacc ggcgcgagat gcgcggcgtg cgtgtcccgc acgatatcta cgtggcggtg
     1681 gatggcgccg atctcgtgcg attgccggac gggcgcttcg ctgtgctgga ggataacctg
     1741 cgcgtcccga gcggtgtctc gtacatgctg gctaaccggc aggtcatgaa acgcaccttc
     1801 tcctcgttgt tcggcagcta cggtgtgcgg cccgtcgatc actatggaca ggccctgctc
     1861 agtacgctgc tctcgcttgc gccggtcgga cgcggcgagc cggtaattgt gctactcacg
     1921 ccgggcgtct acaactcggc ttattttgaa catgcctttc tcgcgcgcca gatgggcatc
     1981 gaactcgtgg aggggcgcga cctgatcgcg catgacaata tcgtctatat gcgcacgacg
     2041 ggcgggctgc ggcgcgtcga tgtgatatac aggcgcatcg atgacgattt tctcgacccg
     2101 ctcgccttcc ggcccgactc ctcgctgggc gttcccggct tgctgaacgc ctatcgcgcc
     2161 gggaatgtcg cgctggcaaa ctccatcggc acgggggtgg ccgacgacaa agcgatttat
     2221 gcctatgtgc cgaccatcat tcgctattat ctcgaccagg accccatcct gcccaatgtg
     2281 gagacttacc tgctcaatga tgcaacgcag cgtacatacg tgctggagcg cctggatgag
     2341 ctggtcgtca aggcggtggg cgaatctggc ggctacggca tgttgatcgg tcccgccagc
     2401 accgaggcgc agcgcgagga gttccgggcg cagatcatcg ccaacccgcg caacttcatt
     2461 gcccagccca cgctggcgct ctcgcgtgcc ccatgcgtcg ttgacaacca gatcgagccg
     2521 cgccacgtgg acctgcgtcc atttgtgctg tatggcgagc atgtcaccat tgtccccggc
     2581 gggctaacgc gcgtggcgct caaacgcggc tccctggtgg tcaattcctc gcagggcggc
     2641 ggcagcaagg acacgtgggt gctgaccgga ccagccccga tggaaggtgg tgcataaatg
     2701 ctttctcgtg ttgccgatag cctctattgg atgagccgct accttgaacg ggcggaacac
     2761 acggctcggc tggtggatgt gcaattgaac cagatgctcg accaggcggg cggggagacg
     2821 aacctgcgct ggcagcaatt gccgcgcagc ctgcgtatgc cgcagccgga gggcgaggtg
     2881 gatgcctaca gcgtgacgca gatgttgacc ttcgatgttg gcaatagcct gtcgattgtg
     2941 tcgtgcatcg cctcggcgcg cgagaacgcg cggcaggtgc gcgagctgat tagctcggag
     3001 atgtgggaac agctcaaccg cctgtttctc aaggtcaaag aggccagtat gcagcaaatc
     3061 tggtacgggg agccgcatcg ctacctgaac tccgtcaagg agggcatcta tctctttcaa
     3121 ggcatcaccg acgcgacgat gagccacagc gaaggctggc attttattcg cgtcgggcgt
     3181 tttcttgaac gcgcaaccgg gacggcggct ttgctggatg agcatttcgc cgcgtttact
     3241 acgcccctgg ccgctccagg cgaggatgag agcgagccgc tggattacct gagctgggtt
     3301 ggactgctga aatcgtgcgc gtcgttcgag gcgtattgca cggtgtatac cgccgtcatt
     3361 cggcctgcca atatcgcgga attcttgctg ctgaatgccg aagcgccgcg ttcgattcgt
     3421 ttctcctgtg ctatgatgca gggcgcgttg caagccatcg cgaaatccac cggcgcgcgc
     3481 aatccgggac gagttgagcg catcatcggc cgcctgcgcg ccgcgctcga ttatgaccag
     3541 atcgaggaaa tcttcggcgg tgacatgcac gagtaccttg aaaatatccg tcgccagtgc
     3601 gggcagatcc atagcggcct gtatcaaacg tatatcgcct atcccatcga ggtcgctttg
     3661 acatcgcgtg gcgcggcgca ggagtaggaa tgtattacac cattcgccat accacgcgct
     3721 ttcgctatag tgcgccgatc aacgaaagca tcatggaagt gcgtatgcag ccgcgcagcg
     3781 aagatggcca gttctgcctg gattttcacc tgcacaccag cccgcgcgcg caaatcatga
     3841 cctaccgggg cgaatttggc aaccgcgtcc accatttcga tatccccaac tcgcatagtc
     3901 agttgactat taccgccgaa tcgctggtcg atctcacccc gcctgcgccg ctgccggggg
     3961 cgcttgatgg attcgcctgg caagaattgg atacgttgac ggctaaagat gaatactggg
     4021 acacgctgat gccgagccac tatgccaccc ccagcctgct gctcgacgaa ctggcgcagg
     4081 agctggaggt agggagacgc gccgatcccc tgacgacgct gcgcgaattg aatagcgcgc
     4141 tctatgacgc ctttgattgg gttccaacgg cgacgcaggt cgattcgcca atcgacgaag
     4201 cgctgcggat gcgcaaaggc gtctgccagg atttcgcgca tatcatgata accctggtgc
     4261 gccagctgcg catcccctgc cgttacgtca gcggctacct aatccagcac ggtcaattag
     4321 agcgggcgga gcgcgctgcc aacgatgcta tgcacgcctg ggtcgaggcg tatttgccct
     4381 ccctcggatg ggtcggattc gatcccacta acgacacgct ggccgatgaa cgccacgtgc
     4441 gtgtggcggt tggccgcgat tacgccgatg ttactcctac gcgcggcatc ttccggggca
     4501 aggccactac cgagctggcc gtgacgatcc gctatgctcc ttcccagacc gcgctgaccg
     4561 aagatgcccc gccggagctt gcctgggtcg ccgccaccca cgacgaatat gaccgcgagc
     4621 aagcgccgtc tcaggtgcag tcccagcctc agcaacagca gcagtgaaga tcagggatgt
     4681 ggacctgaat acatacttca cgcccgtgct ataatcatgc tcataaaagg ggtaagattg
     4741 ttgggtgggg aaataggtag agtatgtttc ccgaagtctt ccgtgataaa gagttactat
     4801 tgtgatcgtc ttacatgctt tgtgggatca tcgcgcagca gacgcaaccg gcaggctgca
     4861 tatctgggcc gagtcgtctc agatgccatt gccggtgggc gctgggcgaa aagccagggg
     4921 caggcagccg caaaaggaag agaagccgcg cgagcatcct tttgcgttac actatgaact
     4981 gctgcgtcaa tcgctgggcg agcttgtagg gagattgcat atctccgggg gcgaggccag
     5041 cacgctcgat gtgcgcctgc cgtccacgga caaagggccg ctcgcttcac cagaattgat
     5101 cctggacgag gtcgaagcgc ttcaagcgac tgcctttctg ccttacaccg tcccgacgct
     5161 ggcgctaacg cccgactttg cct
//
LOCUS       DASZAK010000493         2041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_544408, whole genome shotgun sequence.
ACCESSION   DASZAK010000493 DASZAK010000000
VERSION     DASZAK010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544408"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..>2041)
                     /locus_tag="VGT44_08620"
     CDS             complement(177..>2041)
                     /locus_tag="VGT44_08620"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2580901.1"
                     /translation="ARCFLHFRHGRDDNTLDWQSQDEAAANSIGLETRGTADPAYWMR
                     TYYRHARVIYRRAALLMEHLPAPVSFYRQFRRRRTPIAGTDFILDQGRIDILPGAQFS
                     DTAAILRIFSLMATHGYLLSQAAEDRITDALPALAIHIPEGPYLWNALREVLLGPHAA
                     HALRTMHALGVLEMLIPEFHGIDALVIRDSYHRYTVDEHTFLTIDNVHSLRHPAHDYE
                     QRLAQLLPEIDRLDLFLLSLLLHDTGKGRRSGEHTGQSVELADSFMARLDFDTEERDQ
                     VLKLIRLHLEMSNALRRDIFDAENVRAFSEKIGSPALLKMLTLMTYADIKAVNPEALT
                     PWKAENLWQLYMATANFMDRSVDEVRYHAAIDPTLLNRIRSMVQPGQHEALRQFLEGL
                     PQRYLQTRLPEHIRNHFQLAADLDQNPVQLDLRPHRQLFDLTVIVRDRSRLFADVAGA
                     LAAWGMNIVKAGAFSNDAGVVVDSFQFTDVFRTIELNPSEKDRFLDYLRDVVAQKVPV
                     EQMLDARRHANNSVNAKVDVATRLQFDSESSTHSTILQVVAQDVPGLLRQIALTLSTH
                     KCNIEVALIDTEGETAIDVFYLTQGGVKLTNETQHTLAADLTAAINQLRAPVGA"
BASE COUNT          382 a          571 c          678 g          410 t
ORIGIN      
        1 gcagcaattc tcgaatggtt aattccccct cgtgaggcag cgaatggcct tagtttatcc
       61 cccgcatccg atctcggtgc ggctggttgt gatgcgagcg ggccggcggg ggcggagtcc
      121 ctggtgagtg ggcgagttgc gccgccagac tgaagctttt ggggctccag tctgccctag
      181 gctcctacgg gtgcgcgcag ttgattgatg gcggcggtaa gatcggcagc cagcgtgtgc
      241 tgcgtctcat tggtcagctt gactccgccc tgggtcagat agaaaacgtc gattgcggtc
      301 tcgccttcgg tatcgataag tgcaacctca atgttgcatt tatgcgtgct gagcgtaagc
      361 gcgatctggc gcagcaggcc cggtacatcc tgtgcgacaa cctgcagaat cgtggaatgc
      421 gttgaagact ccgaatcaaa ttgcaggcgc gtcgcgacgt caaccttggc gtttaccgag
      481 ttgtttgcgt ggcgccgggc atcgagcatc tgctccaccg gaaccttctg ggcgaccaca
      541 tcgcgcaggt aatcgaggaa gcggtctttc tcgctggggt tcaactcgat ggtgcgaaag
      601 acgtcagtaa actggaagct gtcgacaacc acgccggcat cgttcgagaa cgctccagcc
      661 ttcacaatgt tcatgcccca ggccgccagc gcgccggcga cgtcggcaaa caagcggctg
      721 cggtcgcgca caatcacggt gagatcgaag agttgccggt gagggcgaag gtcaagctga
      781 acaggattct ggtcgagatc ggcggcaagc tgaaagtgat tgcggatatg ttccggcagg
      841 cgggtctgca gatatcgctg gggaaggcct tcaagaaatt gccgcagtgc ttcgtgctgg
      901 ccgggctgca ccatcgagcg gatgcgattg agaagggttg ggtcgatcgc cgcgtggtac
      961 cgcacctcgt ccacactgcg gtccatgaag ttggctgtgg ccatgtaaag ttgccacagg
     1021 ttctcggcct tccatggcgt cagggcttcc ggattaacgg ccttgatgtc ggcatacgtc
     1081 atcagcgtca gcatcttcaa gagcgccggg ctgccgatct tctctgagaa cgcgcgcacg
     1141 ttttccgcgt caaagatgtc ccggcgcaat gcgttcgaca tctccagatg caggcggatc
     1201 agcttgagta cctggtcgcg ttcctcggtg tcaaaatcca gccgcgccat gaaactgtcg
     1261 gccagttcta cgctctggcc tgtatgctcg ccgctgcgcc ggcccttgcc ggtgtcgtgc
     1321 aggagcagcg acagcaggaa cagatcgagg cggtcgatct ccggcagcag ctgcgccaga
     1381 cgctgttcgt agtcgtgcgc cggatgacgc aggctgtgta cgttgtcgat ggtcaggaag
     1441 gtatgctcgt caaccgtgta gcggtggtag ctgtcgcgaa tcaccagggc gtcgatgccg
     1501 tgaaactcgg gaatcagcat ctccagcacg ccgagcgcgt gcatggtcct cagggcgtgt
     1561 gccgcatgcg gtccgagcag gacctcgcgc agggcgttcc agagataagg accttcaggg
     1621 atgtggatag cgagcgccgg aagcgcgtcg gtgatgcggt cttcggcggc ttgcgagagc
     1681 agatagccgt gcgtcgccat cagcgaaaag atgcgaagaa tggcggccgt atcgctgaat
     1741 tgcgcgccgg gaagaatgtc gatgcgcccc tggtcgagga tgaaatccgt gcccgcgatc
     1801 ggggtccttc ggcgtcggaa ctggcggtag aaactgaccg gcgccggcag gtgttccatg
     1861 agcagcgccg cgcgtcgata aatgacccgt gcatgccggt agtaggtgcg catccagtaa
     1921 gctgggtcgg cagtgccgcg cgtttcaagg ccaatggagt tggccgccgc ctcatcctga
     1981 gactgccaat cgagcgtgtt gtcgtcgcgc ccgtgccgga agtgcagaaa gcagcgcgcc
     2041 g
//
LOCUS       DASZAK010000494         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_295388, whole genome shotgun sequence.
ACCESSION   DASZAK010000494 DASZAK010000000
VERSION     DASZAK010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295388"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1558
                     /gene="radA"
                     /locus_tag="VGT44_08625"
     CDS             143..1558
                     /gene="radA"
                     /locus_tag="VGT44_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV2580902.1"
                     /translation="MPKLRTKFVCQQCGGEQSKWVGKCPDCGAWNTLEEMAETPQSPA
                     QQRRQVAMGGSSALAQGTVTPVVLPNIEPLAQERIQVGYAEIDRVLGGGLVAGSLILV
                     GGEPGIGKSTLLLQVAGNIARDTGSVLYVSGEESIQQVKMRAERLGIAGERLYLLASI
                     EMETIVDAVHRLKPALVIVDSIQTTLAGHVTSAPGSISQVRECTMQLMQLAKNTHTPI
                     FIIGHVTKEGTVAGPKALEHIADAVLYLEGERYHTYRLLRGVKNRFGATQEVGVFEML
                     GEGMVEVPNPSAVFLSDRATGASGSAVVVSMEGTRPILVEVQALVIPSNFGNPRCNTN
                     GIEYSRLQMLLAVLTKRVGLAVNNHDIYANVVGGFTLEEPAIDLGVAAAIASSYREKN
                     IAPDTALIGEVGLSGELRAVGRLGLRIREAAKLGFKRCIVPKAGGGTKIGAELEHAGL
                     PGDFKIFPASTLAVALEVALH"
     gene            1889..3055
                     /locus_tag="VGT44_08630"
     CDS             1889..3055
                     /locus_tag="VGT44_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV2580903.1"
                     /translation="MSIKTIARIIGSLVTLGVAIALYFFDHEITIAFWWFIGISAAAI
                     IFAFIVTPFITVVPYHWMRETSASDLIAAVLGLIVGLIISVLLFTPLSILPGSLGRIM
                     PVVAVVVFSFLGVALGVQRKNDIAHLFQTNWFSRRSRERDRDEEPVKERERGGKDRDR
                     DREKTTPAVHVEASSPQILLDTSAIIDGRIADISQTGFIFGTLVVPRFVLNELQHIAD
                     SADTMRRNRGRRGLEMLQRLQKDATVPIEIRDVDKEDGAEVDGELVKMARSLHCSIIT
                     NDFNLNRVAELQGVKVLNINELANAVKPVLLPGEDIYIKIMQEGKELGQGVGYLDDGT
                     MIVVEGGRQFMGSSIEVSVTRVLQTVAGRMIFAHPRQSGGPSAPSYPPSSTRQK"
     gene            3088..>3683
                     /locus_tag="VGT44_08635"
     CDS             3088..>3683
                     /locus_tag="VGT44_08635"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HEV2580904.1"
                     /translation="MVVSAIIAAAGTSRRMEGRDKLWTPLAGRLTLARTVNVFEASPL
                     IESIVLVVNEERLADAAALRGQEGWGKITAIVAGGARRQDSVRAGLTSLAEAAPATQW
                     VMIHDGARPLVTPALIEAGLLAAQQHHAAIAAVPVKDTIKQGVMGFVQATIDRANLWA
                     VQTPQVFSFALISQAYALIELPDDVTDDAALLERQGHRV"
BASE COUNT          778 a         1059 c         1098 g          748 t
ORIGIN      
        1 gattggaaaa ggcgaatcca ttattcccac tccctgtatt tcccctttac ggcaagctga
       61 tcatgttgta acatatgttc aagtcaaccg gcgaaagtac aaatctcatt atacccgatc
      121 aataagatag gacacttcac ctatgccgaa attacgcacg aaatttgttt gccagcagtg
      181 cggcggagag cagagcaagt gggtgggaaa atgccccgat tgtggcgcgt ggaatacgct
      241 ggaagagatg gcggagacgc cgcagagtcc ggcgcagcag cggcgacagg tggcaatggg
      301 cggttcatcg gcactggcgc aggggacggt gacgccggta gtactgccga acatcgagcc
      361 gctcgcgcag gaacgcatcc aggtaggcta tgccgagatt gaccgtgtcc tcggcggtgg
      421 gctggtggcg ggatcgctga ttctggtcgg cggggagccg ggcatcggca aatcgacact
      481 gctcttgcag gtggcgggca acatcgccag ggataccgga tcggtgctgt acgtttcggg
      541 cgaggaatcg atccagcagg tcaagatgcg cgctgaacgg ctgggcatcg cgggcgagcg
      601 gctctacctg ctggcctcca ttgagatgga gaccatcgtt gacgcggtac atcgcttgaa
      661 accggcactg gtgatcgtcg attccatcca gaccacgctg gccgggcatg tcacatccgc
      721 gccggggagc atcagccagg tgcgcgaatg cacgatgcaa ctgatgcaac tggcgaagaa
      781 cacgcacaca cccattttta ttatcgggca tgtcaccaaa gaaggcaccg tggccgggcc
      841 aaaagccctg gagcatatcg ctgacgcggt actctacctg gaaggcgaac gctaccatac
      901 ctaccggcta ttgcggggcg tgaagaaccg cttcggcgcg acgcaggagg taggagtctt
      961 cgagatgctc ggcgagggca tggtcgaggt tccgaatccc tcggcggtct ttctttccga
     1021 tcgcgccacc ggcgctagcg gctccgctgt cgtggtcagc atggagggaa cgcgccccat
     1081 cctggtcgag gtgcaggcgc tggtcatccc cagcaacttc ggcaacccgc gctgcaacac
     1141 caacggcatc gaatatagcc gcctgcagat gctgctggcc gttctgacca agcgggtcgg
     1201 gctggcggtc aataaccatg atatctacgc taatgtcgtt ggcggcttta cactcgaaga
     1261 gccggctatc gacctgggcg tcgcggcggc cattgcctcc agctatcgcg agaagaacat
     1321 cgcgcccgat accgccctga tcggcgaggt cggcttgtcg ggcgaattgc gcgcggtggg
     1381 tcgcctcggc ctgcgcattc gcgaagccgc caagcttggc tttaaacgct gcattgtccc
     1441 caaagcaggc ggaggtacaa aaatcggcgc ggaactggaa catgcaggat tgccgggcga
     1501 tttcaagatt ttccctgcca gcacacttgc ggttgcattg gaggttgcgc tacactaata
     1561 gcatggaggc acaacttccg ccagaattaa tcaggtaaaa cgattgtcca gagtacagca
     1621 agcgcaggaa gtacgttttt cctcttgatt cctggagtgg aaaggtgtat actccatact
     1681 tgttaacaag ccctcagagc atgtatgggt gcgtagtttg gtaggcgctg tcgataaagt
     1741 cgatattcga caggggagtt tgggccaggc aacatcacca ggacttatca tcatcgttat
     1801 catcaacgac ccggcctgac aatcctacca cacgcaattc cgctctggca cttttccatc
     1861 tcattgcgct gcttcggagg atgaagggat gtctatcaaa accattgccc gcattatcgg
     1921 gtcgctggtg acgcttggcg tcgccatagc attgtacttt ttcgatcacg aaatcaccat
     1981 cgctttctgg tggttcattg gtatttcagc cgctgctatt atttttgcct ttattgttac
     2041 gccgtttatc actgttgtcc cgtatcattg gatgcgcgaa acgtccgcca gcgatctcat
     2101 cgcggctgtg ctcggactga tcgttggact gatcatttcc gtgctgttgt tcacacccct
     2161 gtccattctg cctgggagcc tggggcgcat catgccggtg gtggccgtgg tggttttcag
     2221 cttcctgggg gtcgcgctgg gcgtgcaacg caaaaacgat atcgcccacc tgttccagac
     2281 caactggttc tcgcgccgca gccgcgaacg agaccgcgat gaggagcctg tgaaagagcg
     2341 cgagcgcggc ggcaaggatc gcgaccgcga ccgcgagaaa accacgcctg ccgtccatgt
     2401 cgaagcatct tcgccgcaaa tcttgctgga caccagcgcc atcatcgatg gacgcatcgc
     2461 tgacatcagc cagactggct ttatctttgg cacattagtc gttccccgtt tcgtattgaa
     2521 tgaactgcaa catatcgccg attccgctga caccatgcgc cgcaaccgcg gcagacgcgg
     2581 cctcgaaatg ctgcaacgcc tgcaaaagga cgcgaccgtt cctatcgaga ttcgcgatgt
     2641 cgataaagaa gatggcgccg aggtagacgg cgaactcgtc aagatggcgc gcagcctgca
     2701 ctgctcgatc attaccaacg actttaacct caaccgcgtg gcggaactgc aaggcgtgaa
     2761 ggtcctcaat atcaacgagc tggctaacgc ggtcaagccg gtactgttgc cgggagagga
     2821 catctatatc aagatcatgc aagaaggcaa agaattggga cagggtgtgg gctacctgga
     2881 cgatggcacg atgatcgttg tcgagggcgg acgccagttc atgggttcca gcatcgaggt
     2941 atcggtcacg cgcgtcctac agaccgtcgc cgggcgcatg atctttgccc atcccaggca
     3001 atccggcggg ccgagcgcgc catcgtatcc gccatcctcc acccgtcaga aatagtcatt
     3061 tgaccagggc gaccgccagg gaagtttatg gtagtatccg cgatcatcgc cgcagccggc
     3121 acaagccgtc gtatggaggg ccgcgacaaa ctctggacgc cactggctgg ccgtttaacc
     3181 ctggcgcgca cggtaaacgt gttcgaggca tccccgttga ttgagagcat cgtgctggta
     3241 gtgaacgagg aacgcctggc tgacgctgcc gctctgcgcg ggcaagaagg atggggcaag
     3301 atcactgcaa tcgtcgcggg cggcgcgcgc aggcaagatt cggtacgcgc cggtctgacc
     3361 agcctggccg aggccgcgcc cgccacccaa tgggtcatga tccacgacgg cgcacgtccg
     3421 ctcgtcacgc ctgcattgat cgaagccggg ctgctggcgg cgcagcagca tcacgccgct
     3481 attgccgccg tgccggtcaa ggataccatc aagcagggcg tgatgggttt tgtccaggca
     3541 acgatcgacc gcgcaaatct ctgggccgtc cagacacccc aggtcttctc cttcgcgctt
     3601 atctcacagg catatgctct gatcgagttg cccgacgacg tgaccgacga cgcggctttg
     3661 ctcgaaagac agggacatcg tgt
//
LOCUS       DASZAK010000495         4390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_118142, whole genome shotgun sequence.
ACCESSION   DASZAK010000495 DASZAK010000000
VERSION     DASZAK010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118142"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1626
                     /locus_tag="VGT44_08640"
     CDS             <1..1626
                     /locus_tag="VGT44_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV2580905.1"
                     /translation="AIVGNQDTILRKLQEIILAPQVTRVYNKQQILSMYLNTIYYGEQ
                     AYGADAAAFLYYGLQDKPGHSAASQLDLAQSAMLAGIPSAPIGRDPYLHPVAANQRFL
                     GVLHNMRLQGYITIPQENMAIEEAAQPGFLHHGIIRNNLAPNFANYALNELASTLHVK
                     LPDLARAGLQVYTTLDLPLQNKILKIAQLHIGQLAQAHHMSNAAEVLIDYHNGAIRVL
                     LGNVDPTNPQFGSFDVASQGYRQPGSSFKPYIYATAFADGMSPGDPVLDAPFTIQLCC
                     GLPSYSPQNYDLSFHGLISYRYALQNSFNVPAVKLLTKVGVQNALHTAQEMGITSYEG
                     TPNYTMVLGTLSIRLLDQTSAFGVFANGGVRVPPHAIDTVVDTQGRLVYHFAPVGTRV
                     ISKQVAFIMTNVLSDNSSRTFEFGKCSALYLYSNTQLQCYEGNPGQIRPAAVKTGTSQ
                     DFRDNWTVGYTTDYVMGVWAGNNDNSPMVNITGVDGAGPIWHESMVLAEQGRPVTNFT
                     NPGGVVKRTVHYPEGITTTDWYLAGAPVENWYG"
     gene            1636..1854
                     /locus_tag="VGT44_08645"
     CDS             1636..1854
                     /locus_tag="VGT44_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580906.1"
                     /translation="MGAYSTAVASPADAFQHGSAGRLVEAEPEPIVIGLVDDVGGAAQ
                     GAVGAVRAAQAVAAFAPADQDDFLALFV"
     gene            1855..2208
                     /locus_tag="VGT44_08650"
     CDS             1855..2208
                     /locus_tag="VGT44_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580907.1"
                     /translation="MLVELERGDIIYGRCQSDGGQINGIRIVFLAFPHAAIVRVNDDA
                     RDLIAFLREFNAERVIGKNDAGDVRGGEQPGWGDEGSRAMPAKETRDTWPVIDIDEVV
                     SFETIMPDEHLTLSQ"
     gene            2331..2681
                     /gene="ndhC"
                     /locus_tag="VGT44_08655"
     CDS             2331..2681
                     /gene="ndhC"
                     /locus_tag="VGT44_08655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEV2580908.1"
                     /translation="MGTLPIFVLVALAVLFGLLVMVITAVLGPKKPSPEKLAPYECGI
                     QEIEAPKQRFSVKYLLTGMLFIVFDIEIVSFYPLAILLHDLKLLGLIELLIFLAILAV
                     GYLYVWRKGAFTWD"
     gene            2672..3268
                     /locus_tag="VGT44_08660"
     CDS             2672..3268
                     /locus_tag="VGT44_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEV2580909.1"
                     /translation="MGLKNPWEPVQTFSNTQTPRGRELHVKNGDFGILTTQVNKLIDW
                     GRASSLWPSLFGLACCAIEMMSTSADRFDLARFGAEVFRASPRQADLMIVAGRCSIKM
                     APVLRQIYDQMPDPKWVISMGACASCGGVFNNYAIVQGVDKIVPVDVFVPGCPPTPEM
                     LLYGFNELQRKIREGIPNPPDPLKASGMKLVGPVGEEL"
     gene            3273..3902
                     /locus_tag="VGT44_08665"
     CDS             3273..3902
                     /locus_tag="VGT44_08665"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEV2580910.1"
                     /translation="MASTTITAQTTVETIRERFPQAVIETSDFRGEYTILLRPESLVA
                     VCTYLAKNLRYNFLSSVTAVDWPERTPRYDVVYHLLSLPNRSQLRLKTRIGSRGEEHP
                     AAPSVTGIWPAANWYEREVYDLFGLTFTGHPDLRRILMPMDWSTHPLRKDYPLSGFDL
                     PEPHWGGQVPYDVDPGVGSQTMRTPFSRELNESRSKISREQEPGEAKTT"
     gene            3912..>4390
                     /locus_tag="VGT44_08670"
     CDS             3912..>4390
                     /locus_tag="VGT44_08670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913078.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEV2580911.1"
                     /translation="MKQDIETPYTLEENQVNGQRADTMTINMGPQHPSTHGVLRLILT
                     IEGETVIKAVPDIGFLHTGIEKTAESKTYHQALVVTDRMDYLAPLSNNLAYSLSVERL
                     LGIENEINDKINYVRVILAELQRIASHLVWLGTHALDLGAMSVFLYCFREREMILDV"
BASE COUNT          913 a         1345 c         1245 g          887 t
ORIGIN      
        1 gccatcgtcg gcaaccagga taccatccta cgcaagctcc aggagatcat cctcgcgccc
       61 caggtgacac gcgtctataa caagcagcaa attctctcta tgtatctcaa caccatctac
      121 tacggcgagc aagcctacgg cgccgatgct gccgcatttt tgtactacgg cttgcaagat
      181 aagcctggac acagtgccgc ttcgcagctc gacctggcgc agtctgctat gctggccggc
      241 atccccagcg cgcctatcgg tcgcgatccc tacctgcatc ccgtcgcggc caaccagcgt
      301 tttcttggag tgctgcacaa catgcgcctg caaggataca ttacgatccc gcaggaaaat
      361 atggctatcg aagaagccgc gcagcccggc ttcctgcatc acggtatcat taggaacaat
      421 ctcgcgccca atttcgccaa ctatgccctg aacgagctgg caagtacgct gcatgtcaag
      481 ctgccagacc tggcgcgcgc cgggctgcaa gtctatacta cgctcgacct gcccctgcaa
      541 aacaagatac tgaagatcgc gcaattgcac atcggccagc tcgcgcaggc gcatcacatg
      601 tctaacgcag cagaggtctt gattgattac cataatggcg cgatccgcgt actgctcggc
      661 aatgttgacc cgaccaaccc gcaattcggt tcgttcgacg tagccagcca gggctatcgc
      721 cagcccggct cgtctttcaa gccctatatt tacgccaccg ccttcgccga tggcatgagt
      781 cccggcgacc ccgtattgga tgctcccttt actatccagc tctgctgtgg ccttccgtcg
      841 tattcgcctc agaactatga cctgagcttt cacggcctga tctcctaccg ttatgccctg
      901 caaaactctt tcaacgtgcc agccgtcaag ttgctaacca aagtcggcgt gcagaacgcg
      961 ctgcataccg cgcaggagat gggcattacc agctacgaag gcacccccaa ctatacgatg
     1021 gtactgggga cgctgagcat tcgcctgctt gaccagacct ccgccttcgg cgtattcgcc
     1081 aacggggggg tgcgcgtccc gccccacgcc atcgataccg tcgtcgatac gcaaggccgc
     1141 ctggtttacc acttcgcgcc ggtcggcacg cgcgtcatca gcaagcaggt cgccttcatc
     1201 atgaccaacg tcttgagcga caactcttcg cgtaccttcg agtttggcaa gtgcagcgcg
     1261 ctctacctgt atagcaacac gcaactgcaa tgctacgagg gcaaccccgg ccagattcgc
     1321 ccggcagccg tcaaaaccgg cacctcgcag gacttccgcg acaactggac ggttggctac
     1381 accaccgact atgtgatggg cgtctgggcg ggcaataacg acaactcgcc gatggtcaac
     1441 atcaccggtg tggatggcgc aggcccgatc tggcacgaaa gtatggtgct ggccgagcag
     1501 ggccgccccg tgacgaactt caccaatccc ggcggcgtgg tgaagcgcac ggtgcattac
     1561 ccggagggaa tcacgacgac ggattggtac ctggcaggag cgcccgtgga gaactggtat
     1621 gggtaggccg tacagatggg ggcatattcc accgctgtcg cctcgcccgc tgacgccttc
     1681 cagcacggtt ccgccgggcg gctcgtcgaa gcggaacctg agccaatagt tatcggtctc
     1741 gtcgatgatg ttggtggcgc ggcgcagggc gcggtcggag ccgtgcgtgc cgcgcaggcc
     1801 gttgccgctt tcgccccagc cgatcaggat gacttccttg ccctgttcgt ctgaatgctc
     1861 gtagagcttg agaggggaga tatcatctac gggcgatgcc agtccgacgg cggccagatc
     1921 aacggtattc gaattgtctt cctcgccttc ccacacgctg ctatagtcag ggtgaatgac
     1981 gacgcgcgcg atttgatagc gtttctccgc gaattcaacg ctgaaagggt gatcggtaag
     2041 aatgatgcgg gcgacgtgcg cggcggtgag cagccaggtt ggggagatga gggtagccgc
     2101 gccatgcccg cgaaagaaac tcgtgatacc tggccagtca ttgatattga cgaggttgtc
     2161 tctttcgaga cgatcatgcc tgatgagcat ttgacactct cacagtagct gtgctacgat
     2221 acgaacggac gaagtgtaca tattacacca ggtggggcgc gcttgtgcag tgtagcatac
     2281 cccctgtcga tacacaacaa ctatttgaat gaaatgaagg aatcccgcgt atgggaacac
     2341 tccccatttt tgtgcttgtg gcgctggctg tgctctttgg gctcctggtg atggtgatta
     2401 cagccgtcct ggggccgaaa aaaccatcgc cggagaagct cgcgccctat gaatgcggca
     2461 ttcaggagat cgaagctcct aaacagcgct tctccgttaa atatctcctg accggtatgc
     2521 tgtttatcgt cttcgacatt gaaattgtct cgttttaccc actcgcgatc ctgctgcacg
     2581 atctcaagct gctgggtttg atcgagctgt tgatcttcct ggccatcctg gcggttggct
     2641 atctctatgt ctggcgaaag ggggcattca catgggatta aagaacccct gggagccggt
     2701 gcagaccttc tcgaatacgc aaacgccgcg cgggcgagaa ctgcacgtca agaacggcga
     2761 ttttggtatc ctgacgacgc aggtcaacaa actgattgac tggggacgcg cctcgtcgct
     2821 gtggccatcg ctcttcggcc tggcctgctg cgccatcgag atgatgtcca ccagcgccga
     2881 ccgctttgac ctggcgcgtt tcggggccga ggtcttccgc gcctcaccgc gccaggccga
     2941 cctgatgatt gtggccgggc gctgctccat caagatggcc ccggtgctgc gacagattta
     3001 cgaccagatg cccgatccca agtgggtcat ttcgatgggc gcgtgcgcgt cgtgcggcgg
     3061 cgtcttcaac aactacgcca ttgtgcaggg ggtggataag atcgtgccgg tcgatgtctt
     3121 cgttcccggc tgcccgccca cgcccgaaat gctgctctac ggcttcaacg aactgcaacg
     3181 caagattcgc gagggcattc ccaatccgcc tgatccactg aaagccagtg gcatgaaact
     3241 ggttggcccg gttggagagg aactgtaagg ttatggcaag cacaaccatt acggcgcaaa
     3301 cgacggttga aacgatacgg gagaggttcc cgcaggccgt tatcgaaaca agcgattttc
     3361 gcggcgaata tacgattctg ctacgacctg aatcactggt cgcggtctgt acttatctgg
     3421 cgaaaaacct gcgctataac tttttatcca gcgtcaccgc cgttgattgg ccggagcgga
     3481 cgccgcgcta cgacgtggtc taccacctgc tgtcgctgcc caatcgcagc cagttgcgcc
     3541 tcaagacgcg catcggcagc cggggagaag agcatcccgc cgcgccgagc gtgacgggca
     3601 tctggcccgc cgcgaactgg tacgagcgcg aggtgtacga cctgtttggc ctcaccttca
     3661 ccggccatcc cgacctgcgc cgcatcctga tgccgatgga ctggagtacg cacccgctgc
     3721 gcaaggacta cccgctgtcc ggcttcgatc tgccggagcc gcattggggc ggccaggttc
     3781 cctacgacgt ggaccccggc gtcggcagcc agacgatgcg cacgcctttc agccgcgagt
     3841 tgaacgagag ccgcagcaag atcagccgcg aacaggagcc gggcgaagcg aaaacaacct
     3901 gaagagggcc tatgaaacaa gatatagaga caccctatac attggaagag aatcaggtga
     3961 acggccagcg cgccgacacc atgacgatca atatggggcc gcagcatccc agtacgcatg
     4021 gagtgctgcg actcattttg acgatcgagg gcgagacggt catcaaggcc gtccctgaca
     4081 ttggctttct gcataccggc attgagaaga ccgccgagtc caagacctat caccaggcgc
     4141 tcgtcgtgac cgaccgcatg gattacctgg cgcctctcag caataacctg gcctactcgc
     4201 tctccgtcga acgactgctg ggcatcgaga acgagatcaa cgacaagatc aattacgtgc
     4261 gcgtgatcct ggcggaattg cagcgcatcg ccagccacct cgtctggctc ggcacgcacg
     4321 cgctcgacct gggcgcgatg agcgttttcc tgtactgctt ccgcgaacgc gagatgatcc
     4381 tggatgtctt
//
LOCUS       DASZAK010000496         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_580597, whole genome shotgun sequence.
ACCESSION   DASZAK010000496 DASZAK010000000
VERSION     DASZAK010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580597"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VGT44_08675"
     CDS             <1..381
                     /locus_tag="VGT44_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2580912.1"
                     /translation="LRLRPAPGEWSIHEVLVHLPDSEIFAYERMRRIIAEEHPTLQAF
                     SEEVWANNLGYLHQDAPLALNLFRTMRLSNAALLRTLPASAWERTGTHTERGEMSLYD
                     IFMTFLRHGETHLNQIEQVKQSLK"
     gene            406..942
                     /locus_tag="VGT44_08680"
     CDS             406..942
                     /locus_tag="VGT44_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580913.1"
                     /translation="MSTKFSGYAVTHARNLRQSYVLLVLGLVCLLIAWLLHPPTDTFP
                     LGVLTLGAGLLIAGLLNPYRLLIGGWLGTLVGLAVFLFFSHRIPGSQVFPAYIVAMGV
                     ALIGIGFMGRRGYVKAGAISPGLIVLGIGIVEVLLVGNNTPAGFLPFVLSFWFPGIAL
                     VLCGLFYLAISGRVSGRR"
     gene            complement(948..1802)
                     /locus_tag="VGT44_08685"
     CDS             complement(948..1802)
                     /locus_tag="VGT44_08685"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2580914.1"
                     /translation="MIATASLGWQSTPTAFRQSAHSQPPPCAISSPQSRHEAASVYPQ
                     IKVSLVTRGPLAHAWDKGVADAIRRRLVGLGVEIVEQSKVRAVHANSVVLDQRRELAC
                     DLCIWAASFVVPPLAREAGLPVNERDQVLVDPYLRSISHREIYAIGDAASPVEDPGVA
                     HVRMSAFTAGIMGTHGADCVSAMLKGKTPKPLSFAYLAQAIALGQQHAIFFLLSPDDR
                     PRPPYITGRLGALTRAAVVNFVVTATMGQRRLPGLFAWPGKGRYEQAQRRSLIQEGQH
                     SRPLHHLS"
BASE COUNT          429 a          641 c          630 g          444 t
ORIGIN      
        1 ctgcgactga gaccggcccc cggcgaatgg agcattcacg aagtcctcgt gcatctgccc
       61 gatagcgaga tatttgccta cgaacgtatg cgcaggatta tcgcagaaga acacccgact
      121 cttcaggcat ttagcgagga agtctgggct aacaacctcg gctatctcca ccaggatgct
      181 ccgcttgcgc tgaatctttt ccgaacaatg cgcctctcca atgcagctct gttacggaca
      241 ctacccgcct cggcctggga gcgtaccggt acccatacgg agcgaggcga aatgagcctg
      301 tacgatatct ttatgacctt cctgaggcat ggggagacgc atctgaacca gatcgagcaa
      361 gtgaaacaat cactcaagta atgcggcgag aggagtcata cgccgatgtc aacgaagttc
      421 tccggctatg ccgtcacaca cgcgcgtaac ctgcgtcagt cgtatgtatt gctcgtgctg
      481 ggtctcgttt gcttgctgat agcgtggctg ctgcatcccc ctacggacac ctttccgcta
      541 ggcgtgttga cgctgggtgc cggactattg atcgccggat tgctcaaccc ttatcgtctg
      601 ttgatcggcg gctggcttgg cacgctggtc ggtctggcgg tctttctctt tttcagccat
      661 cgcatccccg gaagtcaggt ctttcccgct tatatcgtcg cgatgggtgt agccttgatc
      721 ggcattggct tcatgggacg ccggggctac gtcaaagccg gagcaatcag ccccggcttg
      781 atcgtgcttg gcatcggcat cgtcgaggtg ctgctcgttg gtaacaacac cccggcgggc
      841 tttctcccat ttgttctctc gttctggttc cccggcatcg ccctggttct ctgtggcctc
      901 ttctatctcg caatcagcgg aagagtaagc gggagaagat agccccatca ggagagatga
      961 tgcaagggcc tcgaatgctg cccttcttgt atgagcgacc gacgctgtgc ctgctcataa
     1021 cgtcccttgc ctggccacgc aaacaggcca ggaaggcgcc gctgccccat tgtggctgtc
     1081 acgacgaaat tcaccaccgc cgcgcgagtg agagcgccta accggcctgt gatgtagggc
     1141 ggccttggcc tatcatcggg agaaagcaga aagaagatcg cgtgttgctg ccccagcgca
     1201 atggcctgag cgaggtaggc gaagctgagc ggtttgggtg tcttaccttt gagcatggca
     1261 ctcacacaat cagcgccatg agtgcccatg atccccgcag tgaaggcgct catgcgtaca
     1321 tgcgccactc ctgggtcctc gacgggcgag gctgcgtcgc caatcgcgta gatttcccgg
     1381 tgggagatcg aacgcaggta aggatcgacg agcacctggt cgcgctcatt gacaggaagt
     1441 ccagcttccc gtgccagagg cggcacgaca aagctcgccg cccagatgca cagatcacag
     1501 gcaagctcgc gcctctggtc gaggactaca ctgttagcgt gcacagctcg caccttgctc
     1561 tgttcgacga tctcgacacc cagtcccacc agtctccgcc ggatcgcgtc ggccaccccc
     1621 ttgtcccagg cgtgagcgag cggtccccgc gtcaccaagc tgaccttgat ctgaggatag
     1681 acgctggccg cctcgtgcct ggattgcggg gagcttatcg cgcaaggcgg cggctgagaa
     1741 tgggccgatt gccgaaaggc tgtaggcgta ctctgccacc ccagggacgc tgtggcgatc
     1801 agtcatactc cccagcgcat agaccaggta gtcatacctc agatcgtgct gctggtgcag
     1861 ctgatcctgc accttgatgc ggtgctggcc aggatcaagg ctggtgactc gcgcctgaag
     1921 aaactgtacc tgcgttttgc gcaggatctg agaaaacgag cgctgaaaga cgcgctaccc
     1981 ggtggcaaac tcatggagcc tggggcgcac ggtgaaggcg tccgactcgt cgatcagggt
     2041 gatctggaca gaatcgctgg ccaccttgcc ggatagacgc aaggtaaaaa gcaagcccgc
     2101 aacccccgcg ccaagaacaa gaatacgtgt ggacgggtgt gtca
//
LOCUS       DASZAK010000497         6937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_509995, whole genome shotgun sequence.
ACCESSION   DASZAK010000497 DASZAK010000000
VERSION     DASZAK010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6937
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509995"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VGT44_08690"
     CDS             complement(<1..278)
                     /locus_tag="VGT44_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580915.1"
                     /translation="MEDIQNAGGAQPATSGHITSKLAPEDGANNTTTPQTTNTGESLE
                     IQFLVVEGFQVGRGAARLDPVDMTRLGCMAGDTILITGSRTTAAKVVP"
     gene            complement(378..728)
                     /locus_tag="VGT44_08695"
     CDS             complement(378..728)
                     /locus_tag="VGT44_08695"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HEV2580916.1"
                     /translation="MKIYVDENIPQKIATRLRAEGYHVEYVTRSVDDEDILEAAYKQS
                     ALLVTSDKDFERLVLDEHRPTAGVIVLRISKRVPMEDRAQIVVNMLRRYRDMLQGTCG
                     SLTESFVDIRRLPR"
     gene            complement(725..967)
                     /locus_tag="VGT44_08700"
     CDS             complement(725..967)
                     /locus_tag="VGT44_08700"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEV2580917.1"
                     /translation="MQDYRQYITSNPKVLFGKPAIKNTRIPVELILDELAAGTSIEEL
                     LEEYPRLTHEAVYAALAFAADTLRREVVYPLDQAAS"
     gene            complement(1034..1882)
                     /locus_tag="VGT44_08705"
     CDS             complement(1034..1882)
                     /locus_tag="VGT44_08705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2580918.1"
                     /translation="MVNLQGKVCLVTGATSGIGRATALGLAQMGATVALVARDRARGE
                     ATRNEIIAQSGNSAIELLLADLSSQQSIRQLAGSIAQNYPRLHVLVNNAGLFNLRRHT
                     TVDGLELTFAVNYLAPFLLTNLLLDTLKASAPARIVNVSSDSHEAGYIRLDRLQSDRG
                     YRPMRAYGQSKLALVLFTYELARRLEGSGVTVNALHPGFVSTNIAQSGIGPIGRAAAR
                     LIFLRGITPEEGAKTSIYLASSPEVEGVTGKYFVKSIPRRSVEVSYDEALQRRLWEES
                     VKLVGL"
     gene            complement(2507..3499)
                     /locus_tag="VGT44_08710"
     CDS             complement(2507..3499)
                     /locus_tag="VGT44_08710"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV2580919.1"
                     /translation="MKIAITGGAGFVGSHLATAYLDAGHDVFIIDTLVNSSPRAAGAL
                     DPRARFYAVDIRDARLHTIFQNERPDIVSHHAVQYAQQFYGTQALIDADVHIRGLLNV
                     LEACVSASVQKLIFASNGTTMYRLMPLSEYEQQGFPLVKEDAPLCPQRSLDISKVAGE
                     WYVRCYTLQHGLAHTILRYADIYGSETAQHPVSYFAAMLAANQRPVIRGSGLDIRDHI
                     FIDDVAQANLCVLERGKNSTLHIASGQGYSQYQFFCAVAQQLSSALLPVYLAGASGEP
                     TAVVMDNTLAWQTLGWKPRIGFTEGIQRMVERLRSEKMQARVEEPVRVEEAVLV"
     gene            3892..5418
                     /locus_tag="VGT44_08715"
     CDS             3892..5418
                     /locus_tag="VGT44_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580920.1"
                     /translation="MRALIKPKFALFLIGMALVTSIAIPMLAISGAFAHAASTTAVSF
                     PAKGGLDCNGDSKIQQSLTYHLCKDFGSGYADQRGYDNGKYVGHDEPTVNFFSNAPGS
                     GNNMQWQITLSKEHPLPATQTFENTPAFWFGLVLCDPNGFPQKPCTPDSDQNHAPRFN
                     GDPNTSGSAFLELQFYPPGFSPWITRISCDLKHWCAALNIDSLECKANFITCNNNCTE
                     PVNFAFIQSDGVPTGPPGPASQTNASFTPNGQTLLMNQGDTIKATIKDTPNGLLTRVD
                     DVTTGQSGFMVASATNGFQSLNVNTCSPTNFSFHPEYSTAKFDNFLNWGLGQGNVGYD
                     IETGHFEIGAHGDKDSDDAPCFPGPTVAGCTGTDSDFDGTSYQPDWPDGTSHTATPLK
                     LSSVAGSGVGPISSPLGQSNYQHVYSTLQFDTEVLATEASCTLKHLSKCTVPPPGASF
                     YPFFAQSGTGSSCVLTFGNDIRGATINDFGRDLQYGAPNIQWSFLDSSGGIRPNPCIP
                     "
     gene            complement(5543..6865)
                     /locus_tag="VGT44_08720"
     CDS             complement(5543..6865)
                     /locus_tag="VGT44_08720"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEV2580921.1"
                     /translation="MGRRDLLRSPGAMRRHIYALVDCVSFYVSCERAFQATLHNKPTI
                     VLSNNDGCIVALDGLAKHIGLKRGQPIFQQQELLRKHDVQVFSSNYSLYQEMSGRVMA
                     LLAEFSPAIEVYSIDEAWLDLSDLAIDDLTEFGRVIKARVLQYTRIPVRVALAPTRCL
                     TKVACELVKGDARYGDVLDLTGFTSQQLDDALSRVAIEDVWGIGPKYARFLSNYGIDT
                     ARDLRDADERWIRKHLTVVGARIQLELRGTACLPLEIERPPKQNIICSKSFGKEIASR
                     EAMEEAVSAYTARAAEKLREQDSLASRVTVFLRTNAFHEGAPQYSNSFTIDLPYPTAF
                     TPELFKYALKGLRAIYRDGYLYKKAGIELSRITPLPVVQPDLFGEVLLTEYYRQARLM
                     GIVDAINRIYGRNTLVFAVQGMTHGWKMRQSKLSGRYTTHWDELMPVF"
BASE COUNT         1557 a         2049 c         1824 g         1507 t
ORIGIN      
        1 atcggcacca ccttcgcggc agtcgtgcgc gagccggtga tcagaatagt atccccggcc
       61 atgcagccca gccgcgtcat atccaccgga tcaagccgcg ccgctccccg tcccacctgg
      121 aacccctcga caaccaggaa ctgtatctca aggctctcgc cagtatttgt cgtctgaggg
      181 gtcgtcgtat tgttcgcgcc atcctccggc gcaagcttgc tggtgatgtg accgctagtg
      241 gcaggctgcg ccccgccagc gttctgtatg tcctccatac ggtatttcct cttccctctt
      301 gcatgaaaaa gtaataatgg ctcttactag taacttacca catgtacgtg aagggcgggg
      361 gaaaagttgg agggagatta acgtggtagg cggcggatat caacaaatga ttcagtaagc
      421 gacccacaag ttccttgcag catatcccta tacctccgca gcatattcac cactatttgc
      481 gctcggtctt ccattggcac ccgcttagaa atacgcaaga caatcacacc tgccgttggt
      541 ctatgctcgt caaggaccag ccgttcaaag tccttatcgg aagtgacaag caatgcgctc
      601 tgcttataag ctgcctctaa aatatcctca tcatctacac ttcgagtcac atactcaaca
      661 tgataaccct ctgctctcaa tcgtgtagca attttctgag gaatattttc atcgacgtaa
      721 atcttcatga tgctgcttga tcaagaggat aaacaacctc cctacggaga gtatcagcag
      781 caaacgccag agcagcgtag acagcttcgt gggtgaggcg tggatattcc tccaagagtt
      841 cttcgatgga tgtgccagct gcgagttcat cgaggataag ctctacagga atgcgtgtat
      901 tctttattgc tggcttccca aacaatactt ttggatttga ggtaatgtat tgtcgataat
      961 cttgcattgt cttatcctcc tcgattatga ttgtatccca agatagataa aatgtctaca
     1021 tcattgcatt atcctacagc cccaccagct tcacactctc ctcccacaac cgccgctgca
     1081 gcgcctcatc gtaggagact tccaccgaac gcctggggat gcttttcacg aaatacttgc
     1141 cggtgacgcc ttcgacttcg ggtgacgacg ccaggtaaat cgaggttttc gcaccttctt
     1201 caggggtgat gccccggagg aagatgagtc gcgccgccgc gcgcccaatg ggacctatgc
     1261 cgctctgcgc gatgttggtg gagacgaaac cagggtgcag cgcgttgacg gtcacgccgc
     1321 tgccctccag gcggcgcgcc agttcgtagg tgaacagcac gagcgccagc tttgattgcc
     1381 cataggcgcg catggggcgg tagcccctgt cggattgcag gcgatccagc cggatgtagc
     1441 ccgcctcgtg cgagtcggaa gagacgttga cgatgcgggc gggcgcgctg gccttcagcg
     1501 tatcgagcag caggttggtc agcaggaagg gcgcgagata gttcacggcg aaggtcaatt
     1561 ccaggccgtc aacggtagta tgacgccgca ggttgaatag tccggcattg ttgaccagca
     1621 cgtgcaggcg tgggtagttc tgcgcaatgc tcccggcgag ctgccggatc gattgctgcg
     1681 aggacaggtc agccagcagc agctcaatag cgctgttgcc gctctgcgcg ataatctcgt
     1741 tgcgcgtcgc ctcgcccctg gcccggtctc gcgccaccag cgcgaccgtc gcgcccatct
     1801 gcgccagccc cagcgcggtt gccctgccga tgccggatgt cgcccccgtc acgaggcaga
     1861 cctttccttg caaattgacc atgctttttg ttccttactc ttcttgaaat accgaaagaa
     1921 cgataaaaga actcggtagc ttcacgccac cgagtccctc acctctcaca gggaaagggc
     1981 tagaaggctt actaactggt ctgccttaga gcagggacaa cgccaagctg ttgcaggaaa
     2041 gtgagagcat ctcggttgac atagtacatg ctcctttgcg tgtgatttcc ttgctcaggc
     2101 tgagtaggtc gggacgaaac tgagagagaa cgtaaagcga cagaaaaaca tttcgatgct
     2161 tgaaatgaga ggttgccaat ggctagtata tcctcaaaac atagcaagtg caagattagc
     2221 actttacaca aaacctgaaa cgagggtcga ttctgtgcgg tttgtagtct ctacttgaag
     2281 cagatgaagc gtcaagcaca cagaaagagg aaagcaacca tgactcccat gctgcatgca
     2341 gtcagtgaga tcgaagtcat gccgtggggt tcctttgtct attttagcga tcccgatggc
     2401 aatcgctggt ccctgcagca actctccagt cgcccctagc ctgcacgcag aggggactgt
     2461 caaaaacagg taggagccgg tataaccggc tcctacacgg ccccacctaa acgagtaccg
     2521 cctcttctac acgaactggt tcttccaccc gagcctgcat cttctcactg cgcaggcgct
     2581 caaccattcg ctgaataccc tcggtgaaac cgatgcgtgg tttccagccg agcgtctgcc
     2641 aggcgagcgt attgtccatg acaacggcgg tcggttcgcc ggatgcgccc gcgaggtaca
     2701 ccggcagcaa cgcgctggag agttgctggg cgacggcgca gaagaactgg tactgtgagt
     2761 agccctggcc cgaagcgatg tgcagcgtgc tgtttttgcc gcgttccagc acgcacaggt
     2821 tggcctgcgc aacgtcgtcg ataaaaatgt gatcgcgaat atcgagtccc gacccgcgaa
     2881 taactgggcg ctggttggcc gccagcattg cggcaaaata ggaaacgggg tgctgggcag
     2941 tctcgctgcc ataaatatcg gcgtagcgca ggattgtgtg cgccaggcca tgttgcagcg
     3001 tgtagcagcg cacataccat tcgccggcca ccttgctgat atccagtgat cgctgcgggc
     3061 agaggggcgc gtcttctttg acgagcggga agccttgctg ttcatactcg gaaagcggca
     3121 taagccggta catggtggtg ccgttcgagg caaagatcag cttttgcacc gaggcgctga
     3181 cgcacgcctc caacacattc aacaggccgc gtatatgcac gtcggcatcg atcagcgcct
     3241 gcgtcccgta gaactgctgg gcatactgca cggcatgatg gctgacgata tcggggcgct
     3301 cgttttgaaa tatggtgtgc aagcgcgcat cgcgaatatc gacagcatag aaacgcgcgc
     3361 gcggatcgag tgcgcctgct gcgcgcggtg agctattgac cagggtatca ataatgaaga
     3421 catcgtgacc ggcgtcgaga tacgcggtgg cgagatgcga cccgacaaag cccgcgccgc
     3481 cggttatggc aattttcatg aacagggtct cctgaatgct ctcactaaaa acaccaggag
     3541 cgcgtcattg cgctcctgaa gaatactgct ttacgaccca ccggctgctc tggctatgcc
     3601 gatggctctc tggcgtcctg ctatttctag tgtagcctcc cggcctattg ctgtttatcg
     3661 attgacctgg ctatcacggg tggatacaca aaatcctctt tctgcctgaa cccagcgttg
     3721 tcggtaaagg aatcactacc atcctgagca agcgccatac cgaataacta ccaattcatc
     3781 actaactcat caccagaaac atacacctgc gtgatacagt gcgcgtcagc cagttctccc
     3841 ttttcatttc ctgactgaag tagttcatta tcaacgagga gattcttcta tatgagagct
     3901 ttgatcaagc caaagtttgc cctctttctg attggtatgg ctcttgtcac cagtatcgcc
     3961 ataccaatgc tggcaatctc cggtgccttc gcccatgctg cctcaaccac tgccgttagc
     4021 ttccctgcga aaggcgggct ggattgcaat ggcgacagca aaatccagca gtctctcacc
     4081 tatcatctct gcaaggactt cggcagcggt tatgcagatc aacgcggcta cgacaatggc
     4141 aaatatgtcg ggcatgacga gccaacggtg aacttcttct cgaatgcgcc cggctccggc
     4201 aacaacatgc aatggcaaat cactctgtca aaggagcatc ccctgcccgc tacccagacc
     4261 tttgagaaca cccccgcgtt ctggttcggc ctggtgctct gcgacccgaa tggtttccca
     4321 caaaaaccct gcactcccga tagcgaccag aaccacgcgc cgcgcttcaa tggcgatccc
     4381 aatacatccg ggtcggcatt cctcgaattg cagttttacc cacccggctt ctcgccgtgg
     4441 attacccgta tcagttgcga tttaaagcac tggtgcgccg cgctcaacat cgatagcctg
     4501 gaatgcaaag ccaactttat tacctgcaac aacaactgca ccgagccggt caacttcgcc
     4561 ttcatccagt cggacggtgt gccaaccggt ccccctggac cagcaagcca gaccaatgcc
     4621 tcgttcactc cgaacgggca gacactcctg atgaaccagg gtgataccat caaggccacc
     4681 atcaaggaca cgcccaacgg gctgctcacc cgcgttgatg atgtcacaac aggacaatcc
     4741 ggcttcatgg ttgccagcgc cacgaacggt ttccagagcc tgaacgtcaa tacctgcagc
     4801 ccgacgaact tcagtttcca cccggaatac tcgacggcga agttcgataa cttcttgaac
     4861 tggggactgg gacagggcaa cgtcggctac gacatcgaga cgggccactt cgagatcggc
     4921 gcgcatggcg acaaagatag cgacgatgcg ccgtgcttcc ctggcccgac cgtcgccggc
     4981 tgcaccggca ccgacagcga cttcgacggc acatcctacc agcccgactg gcccgacggc
     5041 accagccata ccgctactcc cctcaaactc agttcggtcg caggcagcgg cgtcggcccg
     5101 attagctcgc ccctcggcca atcgaactac caacatgtct attccacgct ccagtttgat
     5161 acggaggtgc tggccacaga agcaagctgt accctcaaac acctgagcaa atgcaccgtc
     5221 ccgcctcccg gagcgagctt ctacccgttc tttgcgcaat caggcaccgg ttctagctgt
     5281 gtcctgacct tcggcaacga catccgagga gctaccatca acgactttgg gcgcgatctc
     5341 caatatggcg cgccaaatat ccagtggagc ttcctcgatt ccagcggcgg tatccggccc
     5401 aatccgtgca tcccataatg aagtttcaca aacaaaatcg ataagaaatt gtcatccctt
     5461 cactgcgttc agggcaagct ctgagcgcag cgaagggatg acattcccga tggcggtcat
     5521 gaaacttcat atcttgatcc catcagaata caggcattaa ctcgtcccag tgcgtcgtgt
     5581 agcgaccgga gagcttcgac tgcctcatct tccagccatg cgtcatacct tgaacagcga
     5641 acaccagggt attgcgccca tagatacgat tgatcgcgtc gacgatcccc atcaaacgtg
     5701 cctgccggta gtactcggtg agcaacactt ccccaaagag gtcgggctgc accaccggca
     5761 gcggcgtgat cctgctgagt tcgatgcctg cctttttata gagataacca tcgcgataga
     5821 tggcccgcaa gcccttcagg gcatatttga atagttcagg cgtaaaggcg gtcgggtatg
     5881 gaaggtcgat ggtaaaggag ttggagtact gcggcgcgcc ctcgtgaaag gcgttcgtgc
     5941 gaagaaagac cgtgacgcgc gaggccagcg agtcctgctc gcgcagcttc tccgcggcgc
     6001 gcgcggtata ggcgcttacc gcttcctcca ttgcttcccg gctcgcgatc tctttgccaa
     6061 aggattttga gcagatgatg ttttgttttg gcggtcgctc gatctctaac ggaaggcacg
     6121 cggttccgcg cagctccagc tggattctgg cccccacgac ggtgagatgc ttgcgtatcc
     6181 agcgctcatc cgcgtcgcgt aagtccctgg ccgtatcgat accatagttg ctcaggaagc
     6241 gcgcgtactt cgggccaatg ccccacacat cttctatcgc aacccggctg agcgcgtcgt
     6301 ctaattgctg gctcgtgaac ccggtcagat ccagcacgtc cccgtaccgc gcatctccct
     6361 tgaccagttc gcaggcaacc ttcgtgaggc acctggttgg cgcgagagcg actctcaccg
     6421 gtatgcgcgt atattgcaag acgcgcgcct tgattacgcg cccgaactcg gtcagatcat
     6481 cgattgccag gtccgagagg tccagccagg cttcatcgat ggagtagacc tcgatggccg
     6541 gtgagaactc ggccaacaat gccatcaccc tgcccgacat ttcctggtaa agagaatagt
     6601 tcgaggaaaa gacttgcaca tcgtgtttgc gcagcagctc ttgctgctgg aagatgggct
     6661 ggcctcgctt caaaccaata tgtttcgcca ggccatcgag cgccacaata cagccatcgt
     6721 tattggaaag cacgattgtt ggtttattgt gcagagttgc ttgaaaagcc cgttcgcaag
     6781 agacgtagaa gctgacgcaa tcgacgagtg catagatatg cctcctcatc gcacccggtg
     6841 aacgcaatag gtcacgacgc cccatatctc gaaatcagga tggcctgaca cctcgatagg
     6901 ttgatagttc gggttctccg gtcgcagata catcttt
//
LOCUS       DASZAK010000498         3770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_261667, whole genome shotgun sequence.
ACCESSION   DASZAK010000498 DASZAK010000000
VERSION     DASZAK010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261667"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VGT44_08725"
     CDS             complement(<1..260)
                     /locus_tag="VGT44_08725"
                     /inference="COORDINATES: protein motif:HMM:NF013260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2580922.1"
                     /translation="MTVFPLSAMVGQQQARLALLLNAIDPLIGGVLLCGRRGTGKSTM
                     ARALTALLPEQSGVADCPYFCDPDSSQNMCAACLQRFTRGDTL"
     gene            complement(257..1117)
                     /locus_tag="VGT44_08730"
     CDS             complement(257..1117)
                     /locus_tag="VGT44_08730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin-coupled SDR family oxidoreductase"
                     /protein_id="HEV2580923.1"
                     /translation="MSINQAGSQQKRFQDKVVLITGAAHGMGRAHALAFAREGARLAL
                     CDACRQYDSVPYPLARPEELAQLAGEIESMGCQVIAEQADVTRLAEMQALADRARREL
                     GPIDIVVANAGLYSFALSWEMSEQQWDETVNVDLKGVWITCKVSIPHMLERRAGKIIC
                     ISSTAGFKGMHALAHYVAAKHGVIGLVKTLAIELASYNINVNTVCPTSVDTAMCRNQA
                     IYDAFAGGPGPMATYEHMLELTNQINLFSDRDLLPPEDVSKVVLWLASDEARHLTGTA
                     IPVDAGYLTR"
     gene            complement(1107..1376)
                     /gene="mftA"
                     /locus_tag="VGT44_08735"
     CDS             complement(1107..1376)
                     /gene="mftA"
                     /locus_tag="VGT44_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03969.1"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HEV2580924.1"
                     /translation="MADTIASAPHTPDTEQAPSDLLPGTQEQAAPPIAIETVLAANPA
                     DSTDPNAPKAPVSPPAPAVQPATEEEELDELIIEDFTIDGICGVY"
     gene            complement(1492..1917)
                     /locus_tag="VGT44_08740"
     CDS             complement(1492..1917)
                     /locus_tag="VGT44_08740"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEV2580925.1"
                     /translation="MTDVEARPTAVVPATTTWQEFPVLWRELSDEVWACLHAGGIYRG
                     CRVIMLYWDDVPHVEVGVELPVPCPLTGRVIASTLPAGQVAMTVHWGPYSRLGEAHQA
                     VIDWCAAQGKRKTGTRWEVYGPHNDDPAKVWTEVYWLLA"
     gene            complement(2220..2786)
                     /locus_tag="VGT44_08745"
     CDS             complement(2220..2786)
                     /locus_tag="VGT44_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HEV2580926.1"
                     /translation="MSHRMGLDQAAVVEAAAKLVDEEGIDQLALGRLAERLGIRTPSL
                     YNHVAGLPGLKHNLAIYSARELLDRLTRATIGKSHAEAIVSLADAFRAYARETPGRYA
                     LTLQAPDPSNQKLTAIAQQILGVVQAVLAPYRLSEEEAIHAIRSLRSIVHGFVSLETA
                     GGFGMPVDLDASFHWLIDLFITGLEGIS"
     gene            complement(2776..3504)
                     /locus_tag="VGT44_08750"
     CDS             complement(2776..3504)
                     /locus_tag="VGT44_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2580927.1"
                     /translation="MNVTAYNNNLFQLTRLGAINCYLVREEDGFTLIDTGMSGQAQAI
                     MQAAQKLGLPIARIVLTHAHVDHVGSLDALHEALPDVPVAISERDARFLTGDKSLDAA
                     EPQVPLRGGYPICKTKPTLLLHEGDRIGSLEAIATPGHTPGHLSFLDTRDRAVIAGDA
                     FQTLGGVAVSGTFILLFPLPAMATWHKGLALASARKLLAAQPSVLTVGHGRMLSNPQT
                     AMERAIRVMEQSLAKAERKQSHVA"
BASE COUNT          750 a         1123 c         1109 g          788 t
ORIGIN      
        1 agggtatcac cgcgagtgaa gcgctgcaag caggcggcgc acatgttttg cgagctgtcg
       61 ggatcgcaga aatacgggca atcggcaacg ccgctttgtt ccggcagcag agcggtgagg
      121 gcgcgcgcca tcgtcgattt gcccgtgccg cgccgcccgc agagcaggac gccgccaatc
      181 aacgggtcga tcgcgttcag gagcagggcg agccgcgcct gttgttgtcc gaccatcgcg
      241 ctcaatggga aaactgtcat cgtgttaaat acccggcatc caccgggatg gctgtgccgg
      301 tcaggtggcg cgcctcgtcg gaggccagcc acaaaactac cttgctcaca tcttccggcg
      361 ggagcaggtc gcggtcggaa aagagattga tctggttggt caactccagc atgtgttcgt
      421 aagttgccat tgggccgggg ccgcccgcga aggcatcata aatggcctgg ttgcggcaca
      481 tggccgtgtc aacgctggtt gggcagacgg tattcacgtt gatgttgtag gacgcaagtt
      541 caatcgccag cgttttcact agcccgatta cgccgtgttt ggcggcgacg taatgggcga
      601 gggcgtgcat ccctttgaag cctgccgtcg aggagatgca gatgatcttg cctgcgcgcc
      661 gctcaagcat gtgcggaatg ctgactttgc aggtgatcca tacgcctttc aaatcaacgt
      721 tgacggtttc gtcccactgc tgctcgctca tctcccagct cagcgcgaag gagtagagac
      781 cggcattggc tacgacgata tcgatggggc cgagttcacg tcgcgcgcgg tccgccagcg
      841 cctgcatctc cgccagcctg gtgacatccg cctgctcagc gatgacctgg cagcccatgc
      901 tctcgatttc gccagcgagc tgcgccagtt cttccggtcg cgccagggga tagggtacgc
      961 tgtcgtactg gcggcaggcg tcgcagagcg ccaggcgcgc cccctcgcgc gcgaacgcca
     1021 gcgcatgagc gcgtcccatg ccatgcgccg cgcctgtgat gagcacaact ttatcctgga
     1081 agcgcttttg ctgtgatccg gcctggttaa tagacaccgc agattccgtc gatggtgaag
     1141 tcttcgataa tcaactcgtc cagttcttct tcctccgttg cgggctgaac agccggtgca
     1201 ggcggtgaaa ccggcgcttt tggcgcgttg ggatcagtgg aatctgccgg atttgcggct
     1261 aaaactgtct caatggcaat cgggggggct gcctgttctt gcgtacctgg caacaggtcg
     1321 gaaggcgcct gttctgtgtc cggcgtatga ggagcggaag cgattgtatc agccatcggg
     1381 tctacctcgc ttgtggtaat ggcgactacg aatacagatt gtgacaagta tatcatactt
     1441 caggcctcaa cgaactccac tcgaacgtgg cacgaggctt cgggagagcc atcacgccag
     1501 gagccagtag acctcggtcc acaccttggc gggatcgtcg ttgtggggtc cgtagacctc
     1561 ccatcgtgtg cctgtctttc gctttccctg cgcggcgcac cagtcaatca cggcctgatg
     1621 tgcctccccc aacctcgaat atggtcccca atgcacggtc atcgccacct gcccagctgg
     1681 cagagtcgac gctatcactc gtccggtgag cgggcacggt acgggcaact cgacaccaac
     1741 ctcgacatgt ggtacatcgt cccaatacag catgataacg cggcagcctc ggtagatccc
     1801 gcctgcgtgt aggcacgccc acacctcatc actcagttcc cgccacagca ccgggaactc
     1861 ctgccacgtg gtcgttgcag gcaccaccgc cgtcgggcga gcctcgacgt ccgtcatcgt
     1921 cacctgatag cccatgtttc ctcctgtatc ggcatgcggt atttctcgca aatgacgccc
     1981 cattgatatc ggtcaaagta tagcaaatca gttgaacatt tgtccagcga tcaatatgta
     2041 tcaccaaaaa tgtgtctgac ccccaaaaaa ggcccacact aacacagtta gcgccggaat
     2101 accgatgccg agtccttttc tggcgaacgt tccctgtcca gtctggaagc cccattagcc
     2161 aaatgcggcc aggactgcca gttccaggtt tgcgtttttg agtacggtca ggaatatatc
     2221 tacgaaatcc cctcaagtcc agtaataaac agatcgatca gccagtggaa acttgcatcc
     2281 aggtctactg gcatcccgaa gccgcccgct gtttccagcg agacaaagcc atgcacgatg
     2341 ctgcgcaggc tgcgaatggc gtggatggct tcttcctcgc tgagtctgta gggcgcaagc
     2401 acggcctgga caacacctaa gatctgttgc gcgatggccg tcagcttctg gttgctcgga
     2461 tcgggcgctt gcaaggtgag ggcatagcgc cctggcgttt ccctggcgta ggcgcggaag
     2521 gcatcggcca atgagacgat ggcttccgcg tgagatttcc cgatagtcgc gcgcgtgagg
     2581 cggtcaagta gctcgcgcgc gctgtagatc gccaggttgt gcttgagtcc gggcagtcca
     2641 gcgacgtgat tgtacaggga gggtgtgcga atgcccaggc gttcggctag gcgacccagc
     2701 gcaagctgat cgatgccctc ctcatcgacc agctttgcag ccgcctctac gaccgccgcc
     2761 tggtccagcc ccatcctatg cgacatgcga ctgtttcctc tcggcctttg ccagggactg
     2821 ttccatgacg cgaatggcgc gctccatcgc cgtctgggga ttgctgagca tccggccatg
     2881 tccgacggtt aatacggacg gctgcgctgc cagcagcttg cgcgcgctcg ccagcgccag
     2941 acctttgtgc caggtagcca tcgccggtaa ggggaaaagc aatatgaatg ttcccgatac
     3001 ggctacgccg cccaacgtct ggaaggcgtc cccggcgatc accgcgcggt ctcgcgtatc
     3061 gagaaatgag agatgaccgg gcgtgtgtcc cggcgtggcg atcgcttcga gcgaaccgat
     3121 gcgatctccc tcgtgtagca gaagcgtcgg cttcgttttg cagatgggat agccgccgcg
     3181 cagtggaacc tgcggctcgg cggcgtccag gctcttatcg ccagtcaaaa accgcgcgtc
     3241 gcgctcgctg atggcaaccg ggacatccgg cagcgcctcg tgcagcgcgt caagcgagcc
     3301 gacatgatcg acatgcgcgt gcgtcagcac gatgcgcgca atcggcagtc ccagcttctg
     3361 cgccgcctgc ataattgcct gcgcctgccc gctcatgcca gtgtcgatca atgtaaagcc
     3421 atcctcctcg cgcaccaggt agcagttgat cgcacccaga cgagtcagtt gaaacaggtt
     3481 gttgttgtaa gcagtcacat tcatgacgtg taatgtatcc tttccgttca gtgggctaac
     3541 atgttgacta actatattag ataataaact aatataatta gtttgtcaag agggtagggc
     3601 tgaatcatct aggctgatgt aaagtcacga gaggcatggc gaccgcgagc aatagccaga
     3661 gtgatagttt cgaacttgaa cggccaaaat cagcaagaac ctgtcatcct gagcgcagcg
     3721 aaggatctcg atggtcagcg agtccgatcc ttcgctgcgc tcaggatggc
//
LOCUS       DASZAK010000499         2930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_687377, whole genome shotgun sequence.
ACCESSION   DASZAK010000499 DASZAK010000000
VERSION     DASZAK010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687377"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1132)
                     /locus_tag="VGT44_08755"
     CDS             complement(347..1132)
                     /locus_tag="VGT44_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580928.1"
                     /translation="MKRIASVIVMQARDWRTWLVGPWIVLGATFVICLLIALCIDLLW
                     RGSIPVYSGAVACIAVVMLAVAIGTVGGTFPFAVGFGTRRSDYLLGTLAWSVAYCACW
                     AILMGLLSLIEASVIKNWGVGLHFFHLPIFSDGSPLRQFCWPSPPYDLSCAHSDPNYV
                     SGGSSLQQLWVSFVLLLFMYLLGLLLGSIYQRFGRMGEFMFFGIVFLLLNVFVLLSSY
                     WQWWGTISGWLVQQSAAALGVWLVPVMAIFALASYALLRKATV"
     gene            complement(1129..1887)
                     /locus_tag="VGT44_08760"
     CDS             complement(1129..1887)
                     /locus_tag="VGT44_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580929.1"
                     /translation="MRLITAHLFPTSGEVKVFGAHPYENSRVLSQICAVSDRQKYPNG
                     YRVLDVLDQAAFFFPQWDREYAFALVEAFRLPLSRLMKALSRGMLSTAGSIVGLASRA
                     PLTIFDEPYLGLDAVARSIFYDRLLEDYAEHPRTVILSTHLIDEVSRLLEHILVIDQG
                     RLVLDEEAEALRGRAFTVVGPAVLVDTFTAGKELLARSPLGGQVSATVIGNGDMEDRK
                     YAEALGLELAPVSLQQLLVHLTGEQSARKEVEVR"
     gene            complement(1842..2024)
                     /locus_tag="VGT44_08765"
     CDS             complement(1842..2024)
                     /locus_tag="VGT44_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580930.1"
                     /translation="MNRVVEVSNLTKSFGRVVAVDQVSFSIEAGKIYGLLGATARAKR
                     PSCGSSPPTFFPPAAR"
     gene            complement(2021..2392)
                     /locus_tag="VGT44_08770"
     CDS             complement(2021..2392)
                     /locus_tag="VGT44_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV2580931.1"
                     /translation="MEYLINENRPIFVQIAERIENDIIAGELAEETQVPSTNQFASFY
                     QINPATAAKGVNLLVDQGILYKKRGLGMYVAPGARAKLLEKRRAQFFEQYVVPMFQEA
                     EKLGITMEQVTEMVQRRVEVP"
     gene            2453..2875
                     /locus_tag="VGT44_08775"
     CDS             2453..2875
                     /locus_tag="VGT44_08775"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEV2580932.1"
                     /translation="MSTPSKIHACAQRVYIITFVSVIVISRVPETIGIYWRRSQRDPT
                     ARKADRGSLVVMSFVTGGGVIVACALAAVWTGAAIPWLRPQVTIAGIVVILLGTTLRW
                     WAIFTLGRYFTIDVAVRSTRSVVQSGPYRFVRHPSYTG"
BASE COUNT          641 a          882 c          816 g          591 t
ORIGIN      
        1 catcgacagg tctttgatcg cctgaaattg cgcaaatgac ttattgatat gttctaccgt
       61 aagactcaca gttccttact ccttccaggt tgctcggtcg aacagtgcta ccagttcccg
      121 tatatacacg tctgcgtcaa ggttcggatt ggccacaaag aggggcagca ccgcatcagt
      181 ggctcgtctc ttcattgccc gtcctttaga atattgctat ctgctatatt atatataaca
      241 gatagcaata cgttttgtca agggggtacg tattcagatc aattcaggtg gaaagcctgg
      301 gaaacgtgaa cgcgatctgg ctctctcaac tgctcttgct tgcccgtcat acggttgctt
      361 tgcgcagcag cgcataggac gccagggcaa agatggccat taccggcacc agccacacac
      421 cgagcgctgc cgcgctctgc tggacgagcc agccagagat cgttccccac cactgccagt
      481 agctactcag cagcacaaag acgttgagga gcaggaagac gatgccaaag aacataaact
      541 cccccatgcg gccaaagcgc tggtagatgc tgccgagcag cagtcccagc aggtacatga
      601 acagcaggag gacgaaggag acccacaact gctgcaagga cgagccacca ctgacatagt
      661 tggggtctga gtgtgcgcag gacagatcgt aaggggggga tggccagcaa aactgccgca
      721 agggcgagcc atcgctgaaa atggggaggt ggaagaagtg gagacccacg ccccagttct
      781 tgatcacgct ggcctcgatg agcgagagca gccccatcag gatcgcccag caggcgcagt
      841 atgcgacgct ccatgccagg gtgcccagca ggtaatctga tcggcgtgtg ccaaagccaa
      901 cggcaaacgg aaacgttccg ccgacggtgc cgatggctac agccagcatc acgacggcga
      961 tgcaggcaac tgcgccgcta tagactggga ttgaccccct ccagagcaag tcaatgcaca
     1021 gggcaatcag cagacaaatg acgaaggtag cgcccaaaac gatccaggga cccacgagcc
     1081 aggtcctcca atccctggct tgcatcacta tgacacttgc aatgcgcttc atcgaacttc
     1141 tacctccttt cttgcggatt gttcaccggt cagatggaca agcaactgct gcaacgagac
     1201 cggggcaagt tccaggccga gcgcttcggc gtactttcgg tcctccatgt ccccattgcc
     1261 tatgacggtt gccgaaacct gcccgcccaa cggcgaacgc gcaagcagtt ccttgcccgc
     1321 cgtaaaggtg tcgaccagga ccgccgggcc gacgacggtg aacgcccgcc cacgtagggc
     1381 ttcggcctcc tcgtcgagca ccagccgccc ctggtcgatg acgaggatat gctccaacaa
     1441 ccggctgacc tcgtcgatca gatgcgtcga aaggatgacc gtgcgcggat gctccgcgta
     1501 atcttccagc agccggtcgt aaaagatgct ccgggcgacc gcgtccaggc ccagataggg
     1561 ttcatcgaag atggtcagcg gcgcgcggct ggcaaggccg acgatgctgc cggccgtgga
     1621 tagcatgcct cgggaaagcg ccttcatcag ccgggagagc ggcaagcgga acgcttcgac
     1681 cagcgcgaag gcatactccc ggtcccactg ggggaagaag aaagcggctt gatccagcac
     1741 gtcgagcacg cgatagccgt tgggatactt ctggcggtcc gagactgcgc agatctggct
     1801 cagcacgcgg ctattttcat agggatgtgc tccgaagacc ttcacctcgc cgctggtggg
     1861 aaaaaggtgg gcggtgatga gccgcatgat ggtcgttttg cccgcgccgt tgcgcccagc
     1921 aggccgtaga ttttcccggc ttcgatggag aagctcacct ggtcgaccgc cacaacgcgg
     1981 ccaaacgatt tggtcagatt gctgacttcg acgaccctgt tcatggcact tcaacccttc
     2041 tttgaaccat ctctgtcacc tgctccatcg tgatgcccag cttttccgcc tcctggaaca
     2101 tcggcacgac gtactgctcg aaaaactgtg cgcggcgctt ctccagcagt ttcgcgcgag
     2161 cgcctggcgc cacatacatg ccgagtcctc gctttttgta caaaatgccc tggtccacca
     2221 gcagattcac gcctttggcc gccgtggccg ggttgatctg ataaaaagac gcgaactgat
     2281 tagtggaagg aacctgtgtt tcttcggcta attccccggc gataatgtcg ttttcgatcc
     2341 gctcggcaat ctgcacaaag atcggacgat tctcgtttat caagtactcc atcgcttcat
     2401 cacttatcca gttagtcact cttgtaacta actatagtgc aaaagatgcc cattgtcaac
     2461 accgagcaaa attcacgcct gtgcgcagcg tgtctatatc atcaccttcg tcagtgtcat
     2521 cgtcatctcg cgcgtccctg agaccatcgg catctactgg cggcgctccc aacgcgaccc
     2581 caccgctcgc aaggcagatc gcgggtcact cgttgtgatg agtttcgtga ctggcggagg
     2641 cgtgatcgtg gcttgcgccc tggcagctgt ctggacgggt gcagcgattc cctggctcag
     2701 gccccaggtg acgattgccg gcatcgtcgt cattctgctc ggcaccacgc tgcgctggtg
     2761 ggccatcttc actctcgggc gatacttcac catcgacgtg gcggtgcgct ccacgcggtc
     2821 ggtcgtgcag tccgggccgt atcgcttcgt gcgacatccc tcttacacgg ggtagcgcta
     2881 cgtaagctgt caccagccgc aaagtaactg atgaggtaaa tgacagaact
//
LOCUS       DASZAK010000500         1746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_368101, whole genome shotgun sequence.
ACCESSION   DASZAK010000500 DASZAK010000000
VERSION     DASZAK010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368101"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1663
                     /locus_tag="VGT44_08780"
     CDS             242..1663
                     /locus_tag="VGT44_08780"
                     /inference="COORDINATES: protein motif:HMM:NF025080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEV2580933.1"
                     /translation="MQGKGSAFCETATSLEVRYTGKPVSGWGGLVAVMRYLEQRGVRQ
                     VLGQALPDGRTSPNQIPVLDIGLALFATVLTGGRRFAHLERLRSDEVVQAILGVARMP
                     SAMTLTRYFGGLVRSQIEHLSEVLSQFGSRRLRAPALGAVLDLDSTVFERYGRQQGSL
                     KGYNPRKHGRPSHHPLLAMLAEAKVVLHAWLRSGNTASARGVIAFLAETLAKLPDGFR
                     LYALRADSGFFVTDFLTELERRALPYVIAVRMNPHLRRTVAGIKQWTPLARGLEAAET
                     SYQAHGWQAPRRLVVVREIIRERPEARGRKLLEVPGYTFHVLVTTLSHDPVQTWRFYN
                     SRAECENRIKELKDDFGADGFCLHSFDGTEATFRLICFLFNLIADFKREITQHEAPRL
                     LTLRARLLVIGAIRGADGRRQILRLGLRGRWRQRFAALLARIAALPLATVAQFAADPK
                     NPLPRPWKPPPPRFHPPLLPALK"
BASE COUNT          303 a          545 c          553 g          345 t
ORIGIN      
        1 agtcgatctt gtgaagcatc tcgatcactc aactgacaat caacctgggg aagccgcctg
       61 atcgtgaccc accgattcca caacattgaa caataactca tcgaactgcc gttattgccg
      121 gttcgtcatt tagcccgaaa aaatcagttt gacggcggtg agagacgcat cgtcctgaag
      181 aactcctttg gtatcgttgg gttgtgagac tcggcgataa gccaggcgga gggacaggac
      241 gatgcagggg aagggtagcg ctttttgcga gacggcgacc agcctggaag tgcgttatac
      301 gggcaaaccg gtcagcggct ggggtgggct ggtagcggta atgagatacc tggagcagcg
      361 cggagtgcgc caggtgttag ggcaggcatt gcccgacgga cgcacctcgc ccaaccagat
      421 tccggtgctc gacatcgggt tggctttgtt tgcgacggtg ctgaccggcg gccgccgctt
      481 cgcgcacctc gagcggttgc gcagcgacga agtggtacag gcgatcctgg gcgtggcgcg
      541 gatgccttca gccatgaccc tgacccgcta cttcggcggt ctggtacgca gtcagatcga
      601 acatctgtcc gaggtgctgt cgcagtttgg gtcgcggcgg ctgcgcgcgc cggcgctggg
      661 cgcggtgctg gatttggatt cgaccgtgtt cgagcgctac gggcgccagc agggcagtct
      721 caaaggctac aacccacgca aacacgggcg tccctcccat catccgctgc tggcgatgtt
      781 ggccgaagcc aaggtcgtgt tgcacgcctg gttgcgcagt ggcaataccg ccagcgcacg
      841 tggcgtcatc gcttttttgg ccgagacctt ggccaagttg cccgacggtt tccggctcta
      901 cgcgctgcgc gcggactcgg gtttttttgt cactgacttc ctgaccgagc tggagcgccg
      961 cgcgctgccc tacgtgattg cggtgcggat gaatccgcat ctgcgccgca cggtggcggg
     1021 gatcaagcag tggacgccct tggcgcgcgg tctggaagcc gcggagacca gctaccaagc
     1081 ccacggctgg caggccccgc gccggctggt ggtggtgcgc gaaatcatcc gcgaacggcc
     1141 cgaggcgcgc gggcgcaaac tgctcgaggt tcccggctat acctttcatg tcctggtcac
     1201 caccctgagc catgacccgg tgcagacctg gcgcttttac aacagccgcg ccgaatgcga
     1261 aaaccgcatc aaggagctca aggacgactt tggcgctgac ggcttctgcc tgcactcctt
     1321 cgatggcacc gaagccacct tccgcttgat ctgtttcctg ttcaacctca tcgccgactt
     1381 caaacgcgag attacgcagc atgaggcgcc gcggctgctg acgctgcgtg cccggctgct
     1441 ggtcattggc gccatccgcg gcgccgacgg ccgccggcag attctgcgcc tgggtttgcg
     1501 cgggcgctgg cgccaacgct tcgccgcgct gctggcacgc atcgcggccc ttccacttgc
     1561 aactgtggcg cagttcgcag ccgacccaaa aaatcctctg cccaggccct ggaaaccgcc
     1621 tccgccgcgc ttccatccac ccttacttcc agcgctcaaa tgaggttttt gggtttaatt
     1681 cgctaccagt ggcgaggaca gcgcggggga ttgtggcaca tattccgccg tgcgctgctt
     1741 catgca
//
LOCUS       DASZAK010000501         8320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_544662, whole genome shotgun sequence.
ACCESSION   DASZAK010000501 DASZAK010000000
VERSION     DASZAK010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544662"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VGT44_08785"
     CDS             <1..542
                     /locus_tag="VGT44_08785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV2580934.1"
                     /translation="SNRLRDEWEYCFHMTKQKKFAMYQDAVRKPIGDWAEARLRKLNG
                     KSAERHNSENDSGFGRDLRNWVDKTEVLPGNTISVPLVGRNEGHPAVFPVGLPEFFIK
                     LFTKPGDIVLDPFGGCGSTGIAAKGLGRNVMLIDRKAEYCDIMREKLGSKIDVLPDQP
                     DVLPDPPDVSAQSLVQLYF"
     gene            689..1876
                     /locus_tag="VGT44_08790"
     CDS             689..1876
                     /locus_tag="VGT44_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580935.1"
                     /translation="MANVASSLQDVAAGQPAKSVRDLARLRLITGLTLILVSLVGLFG
                     GDWDIQWHALIGRDRTFTPPHDMILIGIGLSGIVALVAILIETRWAGRQHELRQYTTD
                     FLGLLHSSLGLYLVGFGAVCSAVAFPLDTYWHSLYGVDVSLWAPFHTMIYMGGVLGTF
                     GMIYLLLSAAHLAHQDRATATISYAGVVALLGILLSKFSTFLTPAISGHTLNLSFVSV
                     NLFPFLMALTAVFVCVLAARLLRWTGAATMVVATFLLLWLLISAFVPPMMTLLVQAEH
                     QTYLARAGRIGSNIVPLLGQTPFLLLLSLSIDGVVWLGRRGQWTPAMLNKWVLVAATV
                     SMFFVAGITLILIGLGASAGRGGGGHAILPFLLALILTVPGCLIGGWLSLVISDTLQT
                     LRR"
     gene            1881..2483
                     /locus_tag="VGT44_08795"
     CDS             1881..2483
                     /locus_tag="VGT44_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580936.1"
                     /translation="MRKAIQLILCLLAIASLVVTGVAFYQIVLASTIHGAPVRFVDAT
                     AGPYAIKVALYNDPINAGDDIPFNIAVAPGTPGPLSYQVTASPGPDVPGSLAQGDVNT
                     QQSTAYGVPGSITFVTRGPWTLNIAIQGPAGQGEASIPLTAVAPPAMPGWLAWNIGLL
                     PVYGLLIFWFVQTRRKAGANPQKEMGEERPAPEEVAKGRS"
     gene            2484..3065
                     /locus_tag="VGT44_08800"
     CDS             2484..3065
                     /locus_tag="VGT44_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2580937.1"
                     /translation="MARPADPHRRETILRAAAELFTEQGFSDTRLADIAERAGVVIST
                     LYLYFDSKEEMVRAISRENQQRLLEQLRPVLEHLRGEDDIAQFVEIVCAFARDHRDSI
                     KAFNLDSGLSAVRRGIRTTHGPRIQKGIEIIGQLIDEGYLYPYDPDYVMELLITTVRW
                     VVTMCLSMKEEEEEPFKRFCVQWLSNALLKTKS"
     gene            3193..4593
                     /gene="sufB"
                     /locus_tag="VGT44_08805"
     CDS             3193..4593
                     /gene="sufB"
                     /locus_tag="VGT44_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV2580938.1"
                     /translation="MSQVFDMEQQEYSPGFHVAENYAFKSEKGLSRKTVEQISEMKGE
                     PEWMRKFRLRSLDLFEKRPMPTWGADLSGINFDDIYYYIKPVAEQGKTWDDVPSEIKD
                     TFDRLGIPQAERKFLAGVTAQYESEAVYHKVREDLEKIGVIFTDMDTALRLYPDILKE
                     HFGTVIPPSDNKFASLNSAVWSGGSFVYVPEGVRVEIPLQAYFRINAQNMGQFERTLI
                     IAAPGSYVHYVEGCTAPTYASDSLHSAVVEIKVMRGARVRYTTIQNWSKNVYNLVTKR
                     AAAYGDATMEWVDGNLGSKVTMKYPAVLLMEPGARGDVLSVAFASNGMHQDAGAKITH
                     LAPHTTSQILSKSVSKGTGRASYRGLVRINSGAHHTKSSVRCDALLLDENARTDTYPT
                     IRVEENQTEIGHEATVSKVGEDQLFYLMSRGLDESEAYSLIVNGFIEPITKELPMEYA
                     VELNRLIQLEMSGSVG"
     gene            4774..5511
                     /locus_tag="VGT44_08810"
     CDS             4774..5511
                     /locus_tag="VGT44_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580939.1"
                     /translation="MLTYEDVTAFFLKAAASLNLVTHPEYWTNGRTLEREFACVCHTG
                     PCDETETGNACTLSFTWSTLDTALSLEGPAGVCDFFHEPDEHCPHLHTSDIPPLVLDL
                     SYMSPLNGSGATISEATLLSLAQMLKLRASELSSRTIETRSGVNMGVQENRLQPEALT
                     LQQRVELPIWHPNGMRGLHDEPHSRSIRSIQRHRPAADEEEEDEVEVVADDPRPEDWL
                     PQVMIDVCQDIVRVLDALDAVRAHKNL"
     gene            complement(5542..5907)
                     /locus_tag="VGT44_08815"
     CDS             complement(5542..5907)
                     /locus_tag="VGT44_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580940.1"
                     /translation="MQNAPKRILVVDDEQDILEFLRVILEDEGYVVVTSDKGEYLEQL
                     HNGGLPHLILVDVLLSGKDGRAIVKYLKSQEETKSIPIIMISAHPSSEHTALLAGAED
                     FLAKPFDIDVLLAKIAKFF"
     gene            complement(5939..>8320)
                     /locus_tag="VGT44_08820"
     CDS             complement(5939..>8320)
                     /locus_tag="VGT44_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2580941.1"
                     /translation="QAPGAQVTVEYLARHKNDSWAWLEATGSNYLHDPLIGAIVGNFR
                     NVTESKQIEFALQQEKERQRLLNEASATLVSSLDHQITLQEIARLIVPAFADYCRIAL
                     LDEQGEIKEIMVNHTDPDKAHLVRALYDEYKDIANSAYGLQKLLQSGQPELISDVNGS
                     VLSPVEGHSAILHIIRTLGLTSYMGIPLIARNRVIGALTFSSTRPDRLYTQDDVFFAQ
                     ELARRIALTLDNAQLYQASQEEIAERRQIEQNLRFLAEASKLLASSLDYETTLTHVTY
                     LAVPHIADWCSVDMRTDEGIQQLAVAHVDPEKVKWAKELNQKNPPDMQATSGLPNILR
                     TGKPEFYPVIDDAFLVAIAHNEEELALLRLIGFRSVMVVPLLIEGKAIGAITFVSAES
                     GRYYTQADLSMAEELASRAALAIQNARLYREAQQSRHQLEIILQGVADGILVYTPDNR
                     LLYANEAAAHMTGAASLQELLAASPLEVVSKYELIDERGEPLPSTQLTHRRVFAGEPE
                     AQAVIGYREPGTGQPMRWSLVTSRPVRDENGQVALVISIVHDITERMLIERRKDEFIS
                     MASHELKTPVTSLKGFTNVLQRRLGKQGDELGLHYLARMDAQLNKLTSLISDLLDISR
                     MQSGKLALREEPIDLDALIDETVDNVQAATPTHRLRVEGRTGSQVFGDQDRLGQVFVN
                     LLTNAIKYSPHADMVRVRLFKESDQKQAIVSVQDFGIGIDKMHHEKIFDRFYQVTDPE
                     EKTYPGLGIGLHISSEIVARHQGRMWVKSYKGKGSTFFVALPLLQNKEHMDLP"
BASE COUNT         1742 a         2231 c         2451 g         1896 t
ORIGIN      
        1 ggtcaaatcg gttgagagat gaatgggaat actgtttcca tatgacgaag caaaagaaat
       61 ttgccatgta tcaggatgct gtgcgcaagc caatcgggga ttgggccgag gcgaggctaa
      121 ggaagctcaa tgggaagagc gcggaacggc ataacagcga aaatgacagt ggattcggca
      181 gagatttacg gaattgggtg gacaaaaccg aggtattacc aggaaacacg atttccgtcc
      241 ctctcgttgg tagaaacgag ggacatccgg ctgtatttcc tgtcggattg cctgaatttt
      301 tcatcaagct ctttaccaag ccgggcgaca ttgttctcga tccgtttgga ggttgtggtt
      361 ccacaggtat agctgccaaa gggcttggta ggaatgttat gctgatcgat agaaaagctg
      421 agtattgcga catcatgagg gaaaaattag gcagtaaaat agatgtctta cccgatcagc
      481 cggatgtctt acccgacccg ccagatgttt cagcacaaag ccttgttcag ctatattttt
      541 aagaatctgg tggtttttac catatgtaat attctttgag aaatgttggg agccttccga
      601 gcgctttatc cccttgactt cttcacggtt tttccatata cttgctatta atgaaaaaac
      661 tttttttatt aatagcaagg agtgttctat ggcgaatgtt gcgagttctc tccaggacgt
      721 ggctgctgga caaccagcga agagtgtgag agacctggcg cgcctgcgcc tcatcacggg
      781 gctaacgttg attctcgttt cactggtggg cttgtttggc ggtgattggg atatccagtg
      841 gcacgccttg atcgggcgtg atcgcacctt tacgccgcca cacgacatga tcctgattgg
      901 catcgggtta tcgggcatcg tcgcgctggt cgcaatactg attgagacgc gctgggcggg
      961 acgccagcac gagttgcggc aatataccac cgattttctt ggcctgttgc acagttcgct
     1021 cggcttgtac ctggttggct ttggggccgt ctgctcggcg gttgctttcc cgttggatac
     1081 ttactggcat tccctctatg gcgtagatgt ctcgctatgg gcgcctttcc acactatgat
     1141 ttacatgggc ggcgtacttg gtacgttcgg tatgatctac ctgctgctgt cggcggctca
     1201 cctggcgcat caagaccgcg cgacggcgac tatcagctac gcgggcgtag ttgcgctcct
     1261 gggtatcctg ctctcgaagt tcagtacatt cctgacaccg gccatttccg gacacacgct
     1321 taacctgtcg ttcgttagcg taaatctctt tccattcctg atggcgctga ccgccgtgtt
     1381 cgtatgtgta ctcgcggcgc gcttgctgcg ctggacggga gcagcgacaa tggtggtggc
     1441 cacgttcctc ctgctctggc tgctcatcag cgcgtttgtg cctcccatga tgacgctgct
     1501 cgtgcaggcc gagcatcaga cttacctggc gcgagcgggt cgcattggtt cgaatatcgt
     1561 tcccctgctg gggcaaaccc cgttcctgct gctgctttcg ctcagtatcg atggagtggt
     1621 ctggctgggg cggcgcggcc agtggacgcc tgccatgctg aacaaatggg tccttgtcgc
     1681 cgccacagtc agcatgttct tcgtggcggg catcacgctg atcctgatcg gtttgggcgc
     1741 gtccgcaggg cggggcgggg gtggtcatgc gatcctgcca ttcttgcttg cgctgattct
     1801 gacggttcct ggctgcctta tagggggctg gttgagccta gtgattagtg atacgctgca
     1861 aacgctcagg aggtaagacg atgcggaaag cgattcaact cattctctgc ttgctcgcga
     1921 tcgcttcgct ggtcgtgacc ggtgtggcat tctaccagat tgtgctggcc tcgactatac
     1981 atggcgcgcc agtgcgcttt gtcgatgcta ctgcgggacc gtatgctatc aaggtggccc
     2041 tgtacaatga cccgatcaac gcgggagatg acatcccctt caatatagcg gtcgcccccg
     2101 gcactcctgg gccgctcagc taccaggtga cggccagccc gggaccggac gttcctggct
     2161 cgctggcgca gggtgatgtt aacacgcagc aaagcacggc atatggtgtg ccgggcagca
     2221 tcacctttgt tacacgcggg ccgtggacgc tgaatatcgc cattcagggg cctgccgggc
     2281 agggcgaggc atcgattccg ctgacggcgg tagcgccacc ggccatgccg ggatggctgg
     2341 cgtggaatat tggcctgctg ccggtgtatg gcctgttgat cttctggttt gttcagacga
     2401 ggcgcaaagc gggtgccaac ccgcagaagg agatgggcga agagcgacct gcgccggaag
     2461 aggtcgcgaa agggagatcg tagatggcac gacctgctga cccgcaccga cgagagacta
     2521 ttctgcgcgc ggcggcggag ctgttcaccg agcagggctt tagcgacacg cgcctcgccg
     2581 atatcgccga gcgcgccggg gttgtaatca gcacgctcta cctctacttt gactcgaaag
     2641 aagagatggt acgcgctatc tcgcgcgaga accagcagcg cctgctcgaa caactgcggc
     2701 ctgtgttaga gcatctgaga ggtgaagacg acatcgcgca atttgtcgag atcgtctgcg
     2761 cctttgcaag ggatcaccgc gattcgatca aagccttcaa cctcgatagc ggcttgagcg
     2821 cggtgcgcag gggcatacgc accacgcacg gcccgcgcat tcaaaagggc atagagatca
     2881 tcgggcaact gatcgatgaa ggctatctct acccctacga tccagactac gtgatggagt
     2941 tgctcatcac tacagtgcgt tgggtcgtca cgatgtgcct ctccatgaaa gaggaagaag
     3001 aggagccgtt taaacgattc tgcgtgcagt ggctctccaa tgcgctgctg aagacgaaat
     3061 cttgatcgcc ttgacaatct ccgcgtcacg caatataata cgagtaaatc cgagtaggta
     3121 gctaaggatt aagcgcagtc ggcttttcca tataaacttt cgggcaaaag cctgcatgat
     3181 ggagtagata caatgagtca agtgttcgac atggagcaac aagagtatag tcctggattt
     3241 catgtagcgg agaactatgc gtttaagtct gaaaaagggc tgagccgcaa gaccgttgag
     3301 cagatttccg agatgaaggg cgagccagaa tggatgcgca agttccgcct gcgcagcctg
     3361 gacctgtttg agaagcggcc tatgccaacc tggggcgcgg acctgtctgg tatcaacttt
     3421 gacgacatct attactacat caagccggta gcagagcagg gcaagacctg ggatgatgta
     3481 ccgagcgaga ttaaggatac cttcgatcgc ctgggcattc cacaggccga gcgcaagttc
     3541 ctggcaggcg tgacggcaca gtacgagagc gaggccgtct atcacaaggt gcgcgaagac
     3601 ctggagaaga tcggcgtgat cttcaccgac atggatacgg cgctgcgcct gtatcccgat
     3661 atcctgaaag aacatttcgg cacggttatc ccgccctcgg acaacaagtt cgcctcgttg
     3721 aacagcgcgg tgtggagcgg cggcagcttc gtgtacgtgc cggaaggcgt gcgcgtcgag
     3781 attccgctgc aggcctactt ccgcatcaac gcgcagaata tgggtcagtt cgagcgcacg
     3841 ctgattatcg ctgctcctgg ctcgtatgtc cattacgtgg agggctgcac agcgccgacc
     3901 tacgccagcg acagcctgca cagcgccgtt gtcgagatca aggtcatgcg cggagcgcgt
     3961 gtgcgctaca cgaccatcca gaactggtca aagaacgtct acaacctggt gacgaaacgc
     4021 gccgccgcct atggcgatgc gacgatggaa tgggtcgatg gcaacctcgg ctcgaaggtg
     4081 acgatgaaat atcccgccgt gctgctgatg gagccgggcg cgcgcggtga tgtcctgtcg
     4141 gtggcgttcg cctccaatgg catgcaccag gatgcggggg cgaaaattac ccacctcgcg
     4201 ccgcatacca cctcgcaaat cctgtcgaag tcggtctcga aggggaccgg gcgcgcctcg
     4261 tatcgtgggc ttgtgcgtat caattcaggc gcgcaccata ccaagtccag tgtgcgctgt
     4321 gatgcgctgc tgctcgacga gaatgctcgc accgatacct acccgaccat tcgcgtcgaa
     4381 gagaaccaga cggagatcgg ccacgaggcg accgtctcga aggtcggtga agatcagctc
     4441 ttctacctga tgagtcgcgg cctcgacgag tcggaagcct actcgctgat cgtcaatggc
     4501 ttcatcgagc cgatcacgaa ggaactgccg atggagtacg ctgtcgaact caaccgcctg
     4561 atccagcttg agatgtcggg cagcgtcggc taaaacagca tcgacaacgc gataatgcgg
     4621 ccacgcgacc gtgacggtcg cgtggccgca ttatcgcgtt gtcgatggta tcgtattgac
     4681 acccatcgct tgcgcaagta atatagagac acgccgtaga gattggaaag ccatcgacgt
     4741 tttggagcag atgggcggcg cggagaatac agtatgttaa cctacgaaga tgtgacggcc
     4801 tttttcctca aagcggcggc aagcctcaac ctggtgacac accccgaata ttggacgaat
     4861 gggcgcacgc tcgaacgcga atttgcctgt gtctgtcata ccggcccctg tgatgagacg
     4921 gagaccggca atgcctgcac actttccttc acgtggagta cgttggatac cgctctttcg
     4981 ctggaaggtc cggcgggtgt ctgcgacttc tttcacgagc cggatgaaca ctgcccgcac
     5041 ctgcatacca gcgatatccc gcccctggta ctcgatctat cctatatgtc gccgctgaac
     5101 ggatcgggcg cgactatctc tgaggcaacc ctgctatcgc tggcgcagat gctgaaactg
     5161 cgcgccagcg aattgagcag tcgcaccatc gagacgcgct ctggcgtgaa tatgggtgtg
     5221 caggagaacc gcctgcagcc ggaggcgcta accctgcaac agcgcgtcga gctgcccatc
     5281 tggcatccca acggaatgcg tgggctacat gatgagccgc actcgcgctc catccgctct
     5341 atccagcgcc atcgtcctgc tgctgacgaa gaggaagagg acgaagtcga ggtcgtcgcc
     5401 gatgacccac gcccggagga ctggctgccg caggtgatga tcgatgtctg ccaggatatc
     5461 gtgcgcgtgc tggatgcttt ggacgcggtg agagcgcata agaatctcta gcgaagccag
     5521 ggatgccagg tcaggaagcg ctcagaagaa tttcgcgatt ttcgcgagta agacatcgat
     5581 gtcgaaaggc tttgccagga aatcctccgc tcctgccagc agcgctgtat gctccgagct
     5641 gggatgagcg gagatcatga tgatgggaat agatttcgtc tcttcctggc tcttaagata
     5701 tttcacaatc gcgcgtccgt ctttgccgga gagcaataca tcaaccagga tgaggtgggg
     5761 aagtccgccg ttgtgcaatt gttcgaggta ttcgccctta tctgaggtca cgaccacata
     5821 cccctcatcc tctaagatca ctcggaggaa ttcaagaata tcctgctcat catccacgac
     5881 aaggatgcgt ttgggcgcgt tctgcatcga caatttcctg tctttctctc ctgataggtt
     5941 atgggaggtc catgtgttct ttgttctgga ggagtggcag ggccacaaaa aaagtggacc
     6001 cttttccttt gtagcttttc acccacatgc ggccctgatg ccgcgcgaca atctcgctgg
     6061 aaatatgtaa gcctatgccg agtcccgggt aggttttttc ctctggatcg gtgacctggt
     6121 agaagcgatc aaagattttc tcatgatgca ttttgtcaat gccgatacca aaatcttgca
     6181 cgctcacgat tgcttgcttc tggtcgctct ctttgaagag acgcacgcgc accatgtctg
     6241 cgtgtggaga atacttgatg gcgttggtga gcaggttgac aaagacctgt cccagccggt
     6301 cttgatcgcc aaaaacctgt gagccggttc tgccctcgac gcgcaggcga tgggtcggtg
     6361 tcgccgcctg cacattatct acggtttcgt cgatgagagc atccaggtca atcggctcct
     6421 cgcgcagcgc cagtttgccc gactgcatac gtgagatatc gagcagatcg ctgatgaggc
     6481 tggtgagctt attgagctgg gcatccatgc gcgccaggta atgtagccct agctcatccc
     6541 cctgttttcc caggcggcgc tgcaacacat tggtaaagcc tttcagactc gtgaccggcg
     6601 ttttgagttc gtggctggcc atgctgatga actcgtcttt gcggcgttcg atgagcatac
     6661 gctccgtgat atcgtggacg atactgatga ccaacgccac ctgcccattc tcatctcgta
     6721 ccgggcgcga tgtgacgagc gaccagcgca tgggctgccc ggttcccggt tctctatagc
     6781 caatcacggc ctgggcctcc ggttctccgg caaacacacg ccggtgagtg agctgggttg
     6841 agggtaaagg ctctccccgt tcatcgatga gttcgtactt gcttacgacc tcgagtggcg
     6901 acgctgccaa caattcctga agcgaggcag cgccggtcat gtgcgccgcc gcttcatttg
     6961 cgtacaggag ccgattgtcc ggggtgtata cgagaatccc gtcggctacc ccttgcagaa
     7021 tgatttctag ctgatggcga gactgttggg cttcgcgata gagccgcgca ttctggatgg
     7081 ccagagcggc tctactcgcc agctcttcgg ccatggagag gtctgcctgc gtatagtagc
     7141 ggcccgattc ggcggagaca aaggtaatcg cgccgatggc tttgccctct atcaaaagag
     7201 gaacaaccat gacggagcga aagccgatga ggcgcagcag ggcaagctcc tcttcgttgt
     7261 gcgcgattgc gacgaggaag gcatcgtcga tgactggata aaactcgggc ttgcccgtcc
     7321 gcaggatgtt tggcagcccg cttgtggcct gcatatcggg tggatttttc tggttgagtt
     7381 ctttagccca tttgactttc tctggatcga catgcgcgac cgccagttgc tggatgccct
     7441 cgtcggttcg catatcaacc gaacaccaat cggcgatgtg aggcacggcc aggtaggtca
     7501 cgtgtgtcag ggtcgtctca taatccaggg aggaggcgag caatttgctc gcctctgcca
     7561 ggaagcggag attttgctct atttgcctgc gctccgcgat ctcttcctgc gatgcctggt
     7621 acagttgcgc attatccagg gtgagcgcga tgcggcgcgc cagttcttgc gcgaagaaga
     7681 catcatcctg cgtgtagaga cgatcaggcc gagtggagga aaacgtgagc gcgccgatga
     7741 ctcgattacg cgcaatgagt ggtatgccca tataggacgt gagaccaagc gtgcggatga
     7801 tgtgaagtat tgcggaatgt ccttcaaccg gcgagaggac gctcccattc acgtctgaga
     7861 taagttcagg ctgaccgctc tgaagaagct tctgcaaacc ataggcgctg ttggcaatat
     7921 ctttgtattc gtcataaagc gcccggacta agtgtgcctt atcggggtcg gtatgattga
     7981 ccataatctc tttaatctcg ccctgctcat cgagcagggc gatgcggcag taatccgcaa
     8041 aggcaggcac gatcagccgg gcgatttctt gtaaggtgat ctgatgatcg agcgaagaaa
     8101 ccagggtcgc gctggcttca ttgagcagac gctgccgctc tttttcttgc tggagggcaa
     8161 attctatctg cttgctttca gtcacgttgc ggaaatttcc gacaattgca ccaatcaatg
     8221 gatcatgcag gtagttagaa ccagtggcct ccaaccatgc ccaggagtcg tttttgtgac
     8281 gagcgagata ttccacggtg acttgagcgc cgggtgcctg
//
LOCUS       DASZAK010000502         7705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_261701, whole genome shotgun sequence.
ACCESSION   DASZAK010000502 DASZAK010000000
VERSION     DASZAK010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7705
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261701"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1530)
                     /locus_tag="VGT44_08825"
     CDS             complement(163..1530)
                     /locus_tag="VGT44_08825"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HEV2580942.1"
                     /translation="MTSLLLKNAALIVAMDDEDHQWSNGGIYVVDNAIAQIGQMETLP
                     QEADQVIDARHMVILPGLVNTHHHFYQTLTRNLPAAQNATLFGWLRTHYPIWARLTPE
                     AIYVSTKIAIAELMLSGCTTSSDHCYIWPNGARLDDQAQAAGEMGFRFHAARGSMSVG
                     ESQGGLPPDSVVEEEAAILRDSQRVIEAYHDASRYGMLRVVLAPCSPFSVSPELMRQS
                     AEMARAYGVHLHTHLAETQDEAAYCAASFGRSPVELAEDLGWLGPDVWHAHMVHPGKD
                     EISRLGQTHTGVAHCPSSNMRLASGIAPVRAMRQAGARVGLGVDGSASNDGSHLLAEA
                     RQALLLHRVLGDPAGMTAREALHMATRGGASVLGRDDIGALAPGMAADLVGYRLDSLW
                     LAGGAPHDPLASLVFCHPPTVDLSIINGRVRIHDGILLDVDLPALIERHNMLAVKLVR
                     GESVN"
     gene            complement(1586..3781)
                     /locus_tag="VGT44_08830"
     CDS             complement(1586..3781)
                     /locus_tag="VGT44_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV2580943.1"
                     /translation="MTSYIGASLARPDATGKVTGAARYPADLVREGMLHMQVVLAHRP
                     HARILSIDTQAAMQQPGVVAVLTAADVPNNAYGLIDADQPALCGDKVRFEGDKVAVVV
                     AESLDAAEAAAKLVAVSYEDLPAVTDTETALAAGAPLVHEQLGSNLLLHIPIRKGNIA
                     QGLAEADVILEGTFSTSWQEHAFLQPEAGIAYIDEQGRVVVETAGQWLHEDRRQIAEI
                     LGLPEEQVVVRYAAIGGAFGGREDLSIQHLLALAAWKLRRPVSLVWHREESMIVHHKR
                     HPMTVHCRWGARSDGQVTAVEARVVADGGAYASTSVEVTKVATLFASGCYEVANIAID
                     GYVAYTNNVPSGAFRGFGAPQAQFASEIMMTRLAHALGLDPAEMRRRNIYREGSIEPT
                     QNPLPPGVSALPVLERCMEEARVRLGYGSPEREASRAGSASHLRRGIGIACGIKNVGY
                     SFGFPEQATATVELFGEADLERAVLRFGAADVGQGAHLVMRQVAAETLGLPLEKVTLI
                     AEDSSEAPNAGSASASRMTLMGGRAVYEAAVEAKSKWGFTDEHHIVATRQYRPPATTP
                     LHPVSGAGTPNYCYGYAAQAVEVEVNLLTGQAQVLKIISVHDVGRAINRQQVEGQIEG
                     CLAQALGYALLEHFQVKDGRVLTPYFSTYLLPTVLDMPTEIIPVVLELADPNGPYGAR
                     GMAEMPLVPFAPAVASAIHDATGVWLNQQPMTPERVLAALMERDMPAPG"
     gene            complement(3778..5175)
                     /locus_tag="VGT44_08835"
     CDS             complement(3778..5175)
                     /locus_tag="VGT44_08835"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV2580944.1"
                     /translation="MTMWQHYFQPTSLEEALELHAQHADQARLIAGGTDVLVELSRGV
                     RPTTTLIDISRLHDLKYVREENGYILLGALATHNDVIASNACVTRALPLAQACWEVAA
                     PQLRTRATVVGNLVTASPANDTITALMALGAELVLTHKNGERVVRIDDFYPDFRRTVL
                     QPGELIREIRIPALLSNQRGLFLKLGLRRAQAISVIDIALVVTFSGETVSRASITLGS
                     LAPTIVHAHTVETYLEGKALSEPVCREAGQLALRDASPIGDVRGSATYRQATLTGLVT
                     HGLRRIANGQEAEGWMAQPILLETSYANEAAHEPYNGTISTRINGKPYRLHSEASHKT
                     LLNALREDAGLTGTKEGCAEGECGACTVWLNGQAVMSCMVPAAQAHNASITTIEGLAR
                     GDELHPLQVAFIRCGAVQCGFCIPGMLMAGAKLLDEQPHPTLEQAQIALSGNICRCTG
                     YRKILDAVLSAGGAS"
     gene            complement(5307..5669)
                     /gene="uraH"
                     /locus_tag="VGT44_08840"
     CDS             complement(5307..5669)
                     /gene="uraH"
                     /locus_tag="VGT44_08840"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEV2580945.1"
                     /translation="MGGFLTTHVLDTARGCPAASMAIELWRVAPGGMERRLLRTERTN
                     ADGRTQQTLLAGDEFAAGVYELDFEVGEYFAAQGVATPEIPFLDRVTIRFGIADPEAH
                     YHVPLLVSPWSYSTYRGS"
     gene            complement(5687..6619)
                     /gene="pucL"
                     /locus_tag="VGT44_08845"
     CDS             complement(5687..6619)
                     /gene="pucL"
                     /locus_tag="VGT44_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HEV2580946.1"
                     /translation="MTSENSYQYQIAYGKAHVPVYRVYATPLTGIPAIPESTFTGREN
                     VLFAVEIDVEVFGENFLPAYTQGDNSNVVATDSMKNFILRHALSYEGATLEGYLEELG
                     HQFLATYPVMERLRMSGRELAFTPAQVPAGGGQFGESNVLFSHSNNDYGEAHMDFATV
                     NGVTTMTMHECGRVDMQLMKVTGSAFTHFVRDNYTTLPERSDRPLYIYLDAHWKYGDA
                     ADMHTRYIPSEQVRDAIQVVFHEFVSESIQHLMHEMGKRLLARFPQMTEVSFYGQNRT
                     RDPMVLSETDQKRKVYSDPFPAFGTLKLTMSRGK"
     gene            complement(6660..7190)
                     /gene="uraD"
                     /locus_tag="VGT44_08850"
     CDS             complement(6660..7190)
                     /gene="uraD"
                     /locus_tag="VGT44_08850"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEV2580947.1"
                     /translation="MKRTFTLQEINAFDRQQFVTTLESVFEGPPWIVEQAWQARPFHS
                     LAHLHQNLCAVMEQAPVKQKIALIQAHPDLVGRAALAGTLTPASMNEQASAGLDRLTQ
                     EEIAMFTRYNQAYRDKFGFPFVICVRENKKKSILAGFETRLHHTHNQEIEIALSEIAK
                     IGWLRLQATVQGDTVE"
     gene            complement(7397..>7705)
                     /locus_tag="VGT44_08855"
     CDS             complement(7397..>7705)
                     /locus_tag="VGT44_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580948.1"
                     /translation="ARLHSWNMYHCGFSHTCPDGLPQCTRIANEGFTFTDCGENIGLA
                     GPSNPAWNNIYAVQESMMNEGPTGWHFIHLTSTTLHRIGVGIYVDPNGWVWFTEDIVS
                     "
BASE COUNT         1389 a         2380 c         2340 g         1596 t
ORIGIN      
        1 cgtagccggt gtccacgaga gaaagcgaca agcgaaagta actcaccatc agctctactc
       61 attttctgca cgaacctgta tccttgtgag ggtctctcgc tattgtacca aatcttctga
      121 gggagtgcct catttcgaag acgtatattt tcggccagtt cattagttca cgctctcccc
      181 tctcaccagc tttaccgcca gcatattgtg ccgctcgatc agcgcgggca aatcgacatc
      241 tagcaaaatg ccatcgtgga tgcgcacacg cccattgatg atgctcaaat cgacggtggg
      301 agggtggcag aagaccaggg aggcgagtgg gtcgtggggc gcgcctccgg ccagccagag
      361 cgagtcgagg cggtagccga ccaggtcagc tgccatgccg ggggcgagtg cgccgatgtc
      421 gtcgcggccc agcacggatg cgccgccgcg tgtcgccata tgcagcgctt ccctggccgt
      481 catgcccgcc gggtcgccca acacgcggtg cagcagcagc gcctggcgcg cttccgctag
      541 caagtgcgag ccgtcgttcg aggcggagcc gtccacgccc aggccgacgc gcgcgcctgc
      601 ctgccgcatg gctcgcaccg gcgcgatgcc cgatgccagg cgcatattcg aggaggggca
      661 gtgcgccacg ccggtatgcg tctgccccag gcgcgagatc tcgtccttgc cagggtggac
      721 catgtgagcg tgccatacat ctgggccgag ccagcccagg tcttccgcca gttcgacggg
      781 cgagcggcca aagcttgctg cgcagtacgc cgcctcgtcc tgcgtctccg ccaggtgggt
      841 gtgcaggtgg acgccgtagg cgcgcgccat ttccgcgctt tgtcgcatta attccggcga
      901 gaccgagaag ggcgaacagg gcgcgagaac gacgcgcagc atgccgtagc ggctcgcgtc
      961 atgatatgcc tcaatcacgc gctgcgagtc gcgcaggatg gcggcctcct cctcgaccac
     1021 gctgtcgggc ggcaggccgc cctgcgattc gccgaccgac atcgagccgc gcgcggcgtg
     1081 gaaacggaac cccatttcgc ctgccgcctg cgcctggtcg tccaaccgcg cgccgttcgg
     1141 ccagatgtag cagtggtcgc tcgatgtcgt gcagccggag agcatcagtt cggctatggc
     1201 aatctttgtg ctgacataga ttgcttcggg cgtgagccgc gcccagatcg ggtagtgtgt
     1261 tcgcagccag ccgaacagcg tggcattctg cgctgcgggc aggttgcgcg tcagtgtctg
     1321 gtaaaaatga tgatgtgtgt tgaccaggcc gggcaggatc accatgtggc gcgcgtcgat
     1381 cacctggtcg gcctcctgag gaagcgtctc catctgcccg atctgcgcaa tggcgttatc
     1441 aacgacgtag attccgccgt tgctccattg gtgatcttcg tcatccatcg cgacgatgag
     1501 cgccgcgttt ttgagtagca gcgaggtcat aacttacatc tcctattgcc cagaatactg
     1561 aagatcacgg tcaacaccag gcgctttatc caggagcggg catatcccgc tccatcagcg
     1621 cggccagcac acgttccggc gtcatgggct gctggttgag ccagacgccc gtcgcgtcgt
     1681 ggatggcgct ggcgacggcg ggcgcaaatg gcacgagcgg catctcggcc atgccgcgcg
     1741 cgccatatgg tccgttggga tcggccagtt ccagtacgac cgggatgatt tcggtcggca
     1801 tatccagcac tgtagggagg agataggtgc tgaaataggg cgtcaagacg cgcccatcct
     1861 tgacctggaa gtgttccagc agcgcgtagc cgagagcctg ggccaggcat ccctcgatct
     1921 gtccctcgac ctgctggcgg ttgatggcgc gcccgacatc atgcacgctg atgattttga
     1981 gtacctgcgc ctggccggtc agcaggttca cctcgacctc aacggcctgc gccgcgtagc
     2041 cgtagcaata gttgggcgtg cctgcgccgc tcacgggatg cagaggcgtt gtggcgggcg
     2101 ggcggtactg cctggttgcc acgatgtgat gctcgtcggt gaaaccccat ttgctcttcg
     2161 cctcgactgc tgcctcgtag acggcgcggc cacccatcag cgtcatgcgc gacgccgatg
     2221 cgctgccggc gttgggtgct tcgctgctgt cctcggcgat gagtgtcacc ttctccagtg
     2281 gcaatcccag tgtttccgcc gcgacctggc gcatcacgag atgcgccccc tggccgacat
     2341 ccgccgcgcc gaagcgcaac acagcccgtt caaggtctgc ctctccaaac agctcgaccg
     2401 ttgccgtcgc ctgttctgga aagccgaagg agtagcccac gtttttgatg ccgcaagcaa
     2461 taccgatgcc gcgtcgcagg tgtgatgcgc tcccggcacg tgatgcttca cgctccgggc
     2521 tgccatagcc cagtcgcacg cgcgcctctt ccatgcagcg ctccagcaca ggcagcgcgc
     2581 ttacccctgg aggaagtgga ttctgtgttg gctcaatgct tccctcgcgg tagatattgc
     2641 gccgccgcat ctctgccggg tcgagtccca gcgcgtgcgc cagccgcgtc atcatgatct
     2701 cgctggcgaa ctgggcctgg ggcgcgccga agccccggaa cgcgccgctg ggaacgttgt
     2761 tggtataggc aacataccca tcgatggcta tattggccac ctcgtagcac ccgctggcga
     2821 acagggtggc taccttggtg acttcgacac ttgtcgaagc gtaggcgccc ccatcggcca
     2881 cgacgcgcgc ctccaccgcc gtcacctggc catcactgcg cgcgccccag cggcaatgca
     2941 ccgtcatggg atgccgcttg tggtggacga tcatcgactc ctcgcgatgc cacaccagcg
     3001 aaaccgggcg gcgcagcttc caggccgcca gggccagcag gtgctggatc gagaggtctt
     3061 cgcgcccgcc gaacgcgccg ccgatagcgg cgtagcgcac aacgacctgc tcttccggca
     3121 accccaggat ttccgcgatt tgccgacggt cttcatgcag ccattgcccc gccgtctcga
     3181 cgaccacgcg cccctgctcg tcgatgtaag cgatgcccgc ctccggttgg agaaaggcat
     3241 gctcctgcca ggatgtgctg aacgtgcctt ccaggatcac atccgcctcg gctaagccct
     3301 gggcgatgtt gcccttgcga atagggatat gcagcagcag gttgctgccc agctgttcat
     3361 gtacgagcgg cgcgccagcc gctagggcag tctcggtgtc tgtcacggct ggcaggtcct
     3421 cgtaactgac cgctaccagc ttcgctgcgg cctcagccgc gtccaggctt tccgccacga
     3481 ccaccgccac tttgtcgcct tcaaagcgca ccttgtcgcc gcacagcgcg ggctgatccg
     3541 cgtcgatcag cccataggcg ttgttcggga catccgccgc cgtcagtacg gccacgacgc
     3601 cgggctgctg catggcggcc tgggtgtcga tcgagaggat gcgagcgtgg ggccggtgcg
     3661 ccaggacgac ctgcatatgc agcatccctt ctctcacgag gtctgccggg tagcgggctg
     3721 cgccggtcac tttgccggtt gcgtctggcc gggccagcga cgctccgata tatgaagtca
     3781 tgacgcacct cctgcgctca acaccgcgtc gagaattttc ctatagccag tgcaacggca
     3841 gatgttgccg ctcagcgcga tctgcgcctg ttcaagcgtg ggatgtggct gctcgtccag
     3901 caatttcgcc ccggccatca gcatgcctgg aatacaaaag ccgcactgca ccgccccgca
     3961 cctgatgaag gccacttgca ggggatgcag ttcatctccg cgcgccaatc cctcgatggt
     4021 cgtgatcgag gcgttgtgcg cctgcgccgc cgggaccata cacgacatca ccgcctgccc
     4081 gttgagccag accgtacacg caccgcactc gccctccgcg cagccttctt ttgtgccggt
     4141 cagaccagca tcctcgcgca gcgcgttcag caaggtcttg tgtgatgctt cgctgtgcag
     4201 ccggtagggc ttgccgttaa tgcgcgtaga gatcgtgccg ttgtatggct cgtgcgccgc
     4261 ttcgttggcg tagctggttt ccagcaagat cggctgcgcc atccatcctt cggcctcttg
     4321 tccgttggcg atacgccgca gtccgtgcgt caccagcccg gtcagcgtgg cctggcggta
     4381 ggtagccgat ccgcgcacgt cgccgatggg agaggcatcg cgcaaggcga gctgcccggc
     4441 ctcgcggcag acaggctcgc tcagcgcctt gccctcaaga taggtttcga cggtatgcgc
     4501 gtggacaatc gtgggcgcca gcgagccaag cgtgatgctt gcccggctca ccgtctcgcc
     4561 ggagaaggtg acgaccagcg cgatatcgat gaccgagatg gcctgcgcgc gccgcagccc
     4621 aagcttgagg aacaggccgc gctggttgga caacaaggcc gggatgcgaa tttcgcgaat
     4681 cagctcgccg ggttgcaaga ccgtgcgcct gaaatcggga taaaagtcgt cgatcctgac
     4741 gacgcgctcg ccgtttttgt gggtcaagac cagctctgct cccagcgcca tcagcgccgt
     4801 gatggtatcg ttggcgggcg aggccgtcac caggttgccg acgactgtgg cgcgcgtgcg
     4861 caactgcggc gcggcaactt cccagcaggc ttgcgccagg ggcagcgcgc gcgtgacgca
     4921 ggcgttagag gcgatcacgt cgttgtgggt ggccagcgcg ccgagcagga tatatccgtt
     4981 ctcctcgcgc acatatttga gatcatgcag cctggagata tcgatcaagg tagtcgtcgg
     5041 gcgcacgccg cgcgataact caaccagcac gtccgtgccg cccgcgatca acctggcctg
     5101 gtccgcgtgc tgcgcatgca gttccagcgc ctcctcgagg ctggttggct gaaaataatg
     5161 ctgccacatc gtcataatcg acctccttcg acgagcaaaa ggcgtgtagg ggcgcaattc
     5221 attgcgcccg cttgtcagac cgcttcgact gggacgaggg cgcaatgaat tgcgccccta
     5281 catagctttt gcatattgac gtttttttag cttccccggt acgtactata tgaccagggc
     5341 gagacgagca atggcacgtg ataatgcgcc tccgggtcgg caatgccaaa acggatcgtc
     5401 acgcgatcca ggaaagggat ctctggcgtt gctacgcctt gtgctgcgaa atactcgccg
     5461 acctcaaaat ccagttcata gacaccggcg gcgaactcgt cgcccgccag cagcgtctgt
     5521 tgtgtgcgcc catcggcgtt cgtgcgctct gtcctcagca ggcggcgctc catgccgcct
     5581 ggagcgacgc gccagagttc gatggccatg cttgcggcag ggcagccgcg cgctgtatcc
     5641 aggacatgtg tggtaagaaa tccacccatg aatccctcgt atgtgcttat ttgccccgcg
     5701 acatcgtcaa tttcagcgta ccgaaagccg ggaacgggtc gctgtagacc ttgcgtttct
     5761 ggtcggtctc agataagacc atcggatcgc gagtgcggtt ctgcccgtag aaagagacct
     5821 cagtcatctg tgggaagcgc gccagcagcc gcttgcccat ttcgtgcatc aggtgctgga
     5881 tcgactcgct cacaaattca tggaagacta cctgaattgc gtcccgcacc tgctcgctgg
     5941 ggatgtagcg cgtgtgcata tccgccgcgt cgccgtactt ccagtgcgca tccaggtaaa
     6001 tgtacagggg acggtcgctg cgttcgggca gcgtcgtgta gttgtcgcgc acgaaatgcg
     6061 tgaatgcgct gcccgtcacc ttcatcaact gcatatcgac gcgcccgcat tcgtgcatcg
     6121 tcatagtggt gacgccgttt actgtggcaa aatccatgtg cgcctcgcca tagtcgttgt
     6181 tcgagtggct gaagaggacg ttgctctcgc cgaattgccc acccccggcg ggaacctggg
     6241 cgggtgtgaa ggccagttct ctgccgctca tacgcaggcg ctccatcacc gggtaggtcg
     6301 ccaggaattg atgccccagc tcttcgagat agccctccaa cgtcgcgcct tcatacgaga
     6361 gcgcgtggcg caggatgaaa ttcttcatgc tatcggttgc cacgacgttc gagttgtcgc
     6421 cctgcgtata agcaggcagg aaattttctc caaatacttc gacatcgatc tccaccgcga
     6481 aaaggacgtt ctcgcgcccg gtgaaggtcg attccgggat ggctggaatg cctgtcagag
     6541 gtgtggcgta gacgcggtag acaggcacgt gcgctttgcc atacgcgatc tggtactgat
     6601 acgagttctc gcttgtcatg gttattctcc tgctatttgc gtaaaaatat gtatcagttc
     6661 tattctactg tatcaccttg aacggtagct tgcaggcgca gccagccaat cttggcgatt
     6721 tcgctcagag caatctcgat ttcctggttg tgagtgtgat ggaggcgcgt ctcaaaaccc
     6781 gccaggatgc tcttcttttt gttttcacgg acgcaaatca caaatggaaa accgaattta
     6841 tcccggtacg cctggttgta gcgcgtgaac atcgcgatct cttcctgcgt gagccggtcc
     6901 agccccgcgg aggcctgttc gttcatcgag gccggtgtca gggttcctgc cagggcagcg
     6961 cgcccgacaa gatcaggatg cgcctggatc aaggcgattt tttgcttgac cggcgcctgt
     7021 tccatcactg cgcacaaatt ctgatgcagg tgcgcgaggc tatgaaatgg cctggcttgc
     7081 catgcttgct ccacgatcca gggcggcccc tcaaagaccg attccagggt cgtcacaaat
     7141 tgctgccggt caaacgcatt tatttcttgc agggtgaatg ttctcttcat ctccttcaaa
     7201 tttctgatag acggaaaacg atttttcatt gaattgagta taatcggtgc attgtgggtt
     7261 gtcaagggtg aatgtgacca tattctggcg taggaatgta tgatctgacc aaattttact
     7321 tgagaaattc gggcatttta tcaaaatgga aaatgccatt tcttatggtg gaaagaatta
     7381 tggaaaatgt agcaggttag ctgacgatat cttcggtgaa ccagacccac ccgttcgggt
     7441 caacgtagat gccgacgccg atacgatgca gcgtggtgct ggtcagatgt ataaagtgcc
     7501 agccagtcgg cccctcgttc atcatgcttt cctgtaccgc gtagatattg ttccaggccg
     7561 gattggatgg cccggcgagg ccgatatttt ctccgcagtc ggtgaaggtg aagccttcgt
     7621 tggcgatgcg tgtgcattgc gggaggccat cggggcaggt atggctaaag ccgcagtgat
     7681 acatgttcca gctatgcagc cgcgc
//
LOCUS       DASZAK010000503         4290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_368123, whole genome shotgun sequence.
ACCESSION   DASZAK010000503 DASZAK010000000
VERSION     DASZAK010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368123"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..905
                     /locus_tag="VGT44_08860"
     CDS             84..905
                     /locus_tag="VGT44_08860"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEV2580949.1"
                     /translation="MQPVMITAAMVGAEVTRDQQPNLPISPQEIIEAAVGSYEAGAAI
                     IHLHVRDAEGNASQDAGIFREVVEGIRARCDVITQVSTGGAVWMSADERLQSIECRPD
                     MATLTTGTVNFGDGVFMNDRGLVETFARRLLDYGIVPEIEIFDAGMLEEATRLRNMGL
                     ITDPIQFDFVMGVPGGIGADPAHLIHLIRFLPPESTWSVAGIGRHQLTLGTIALAMGG
                     NVRVGFEDNIYYRKGQLAKTNAELVARIARIAQELDRPVATPAQAREMLHLDRYK"
     gene            complement(911..1699)
                     /gene="map"
                     /locus_tag="VGT44_08865"
     CDS             complement(911..1699)
                     /gene="map"
                     /locus_tag="VGT44_08865"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEV2580950.1"
                     /translation="MAVTLKSRQEIAQLRQAGRIVAETYEILRPYVKPGTSTAELDQI
                     AEDYIRSKGAKPIYKGYGARPARNGLPAIPAFPATICVAVNDVICHGIPSRKEILREG
                     DIIGVDIGVLYKGWVGDACMTFAVGKLDSESQRLMDVAKRCLELGIEQARPNKQLGDI
                     GAAIQTYAESQGFSTVREYVGHGVGHSLHEDPQVPHYGQAGTGLRLRPGMVFTIEPMI
                     NAGGYQTKALSDKWTVCTADGSRSAQFEHTIAITEGEPELLTVI"
     gene            1884..2177
                     /locus_tag="VGT44_08870"
     CDS             1884..2177
                     /locus_tag="VGT44_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2580951.1"
                     /translation="MTITCHLRVLLAKTNVERSKLGKPALSLRSLAKESGVSLSVLAS
                     LHTGRSQRVDYTTIDRLLTYFSHYLNVSVDDLFVWEKPGREGMVVDVRREVLT"
     gene            2358..3287
                     /locus_tag="VGT44_08875"
     CDS             2358..3287
                     /locus_tag="VGT44_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2580952.1"
                     /translation="MNLPIETEDIGKRYGKVTAVKHLSLRVEEGEIYAFLGLNGAGKT
                     TTIRMLLGMIKPTWGTARVLQTRVRLGSREPWAAVGYLVETPHSYQELTVLENLEVAR
                     RLHPGTPPEAVGRMVERMGLAAYADRQAGTLSQGNAQRLGLAKALLHAPRLLLLDEPA
                     NGLDPAGIVEIRELLLELTREQGVTVFLASHILAEVSRLAQRIGVLHQGRLLQELDID
                     ELEQNRHRRLLLRARDMEAARLALTTAGHPAAMLRDGTLELTDLRSLEHPDDIASLLV
                     QAGAPPTQVLVEEEDLEQYFLRLVGMNGGQNHA"
     gene            3280..4062
                     /locus_tag="VGT44_08880"
     CDS             3280..4062
                     /locus_tag="VGT44_08880"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2580953.1"
                     /translation="MHDLTNAVWIEVRKATRSRMPLFTALGFLVLAFGLAFLMFIYKD
                     PTFARSSGLISAKANLAGGAATWPYYLGILGQAIAIGGILLFSLVESWVFGREFVDGT
                     LKDLLAVPVARATILLAKFLVVVLWSLLLTVMLFLVSLLLGAAIGLSQGTTEVFWHGT
                     ATLAVAACLVIVDVFPFAFFASVGRGYLLPMGVTFLVLVVGNVVAIAGWGNYFPWAVP
                     ALYAQLTGKGNLDAASYLLVLLTGLAGMAGTYLWWRFADQSR"
BASE COUNT          928 a         1106 c         1240 g         1016 t
ORIGIN      
        1 gtcaatatcg acatagatcc tgaacgtagt gaaggatgac aacataagtg gagagactcc
       61 ttattccaga aaggatcaac ttcatgcaac cagtcatgat aacagcggca atggtcggcg
      121 cagaagtaac cagggatcaa caaccaaatc tacccatatc accgcaagag atcattgagg
      181 cggctgtggg gagttatgaa gcaggggcag cgatcattca tctccatgtg cgtgatgctg
      241 aggggaacgc ttctcaggat gcaggtattt tccgcgaggt agtcgagggc attcgcgccc
      301 gctgcgatgt cattacccag gtatcgacag gtggcgctgt atggatgagc gccgacgaac
      361 gcctgcaaag tatcgagtgc cggccggata tggcgacgct cacaaccgga acagtcaact
      421 ttggcgatgg cgtcttcatg aacgaccggg gcctggtaga aacctttgcg cgtcgattgc
      481 ttgattatgg tattgtgccg gaaattgaaa tctttgacgc ggggatgctg gaagaggcca
      541 cgcggttgcg caacatgggc ctgattacgg acccgataca gttcgatttt gtgatgggtg
      601 tccctggcgg tataggcgca gatcccgccc acctcattca tctcatacgc ttcctgccgc
      661 ctgagagtac ctggagcgta gccggtattg ggcgccatca attgaccctt ggtacgatag
      721 cgcttgcaat gggaggtaat gtgcgggtgg gcttcgaaga caatatttat tatcgtaaag
      781 gccaactggc aaaaaccaat gcagaattag tcgctcgtat cgcccgcata gcccaggagc
      841 tagaccgacc tgtagcaaca cccgcgcagg caagagaaat gttgcacctg gataggtata
      901 aatgaaggaa ttagatcacg gtaagtaatt ccggttcgcc ttccgtgata gcgatcgtat
      961 gctcgaattg ggcagaacga gaaccatcag ccgtacagac ggtccattta tctgacaagg
     1021 ctttcgtctg gtatccacct gcattgatca tgggctcaat tgtgaaaacc atgcctggtc
     1081 ggagtctcaa cccggtaccc gcttgaccat agtgaggcac ctgggggtcc tcgtgcaacg
     1141 aatggccgac accatgacca acatactctc gcaccgtcga aaatccctga gactcagcat
     1201 atgtctgaat ggcagcaccg atatcgccaa gctgcttatt tggtcgtgct tgctcgatac
     1261 ccagttccag gcaacgcttc gctacatcca taagtcgctg cgattcgctg tcaagcttcc
     1321 ccacagcgaa cgtcatgcac gcatcgccga cccacccttt atagagcacc ccaatatcaa
     1381 cgccgatgat gtctccctcg cgcagaatct ctttgcggct ggggatacca tgacaaatca
     1441 catcgttgac ggcgacgcaa attgtggcag ggaatgcagg tatagctggc agtccattcc
     1501 tggcagggcg agcgccgtac cctttgtaga ttggttttgc gcctttgcta cggatatagt
     1561 cctcggcgat ctggtcaagt tccgccgtcg aagtaccggg cttgacgtat ggacgtaata
     1621 tttcgtatgt ttcagcaacg atacgcccgg cctggcgcag ttgcgcgatc tcttgccgtg
     1681 attttaatgt aactgccatg tagtcctctc atatgctcga aagggttacc cccgagtttc
     1741 ttgatctggt ttctctttgt attctatcac ctcagttgtt ataaatcaat acaatgtatt
     1801 gtaatcccac aacatatctg gtacattacg cttaaagagc atttttgcta catactcaaa
     1861 aggcttccag aggaggaact tttatgacta tcacttgcca tctacgcgtg ctgcttgcca
     1921 agacaaatgt cgaacggagc aaattgggaa aaccggcact cagcctgcgc agccttgcaa
     1981 aggaaagcgg cgtttcttta agcgtcctcg cgtccctcca tactggacgc agccaacggg
     2041 ttgattatac cactatcgat agacttctta cttacttcag tcattacctc aatgtcagcg
     2101 tagatgattt gtttgtgtgg gaaaaaccgg ggagggaagg gatggtggta gatgtaaggc
     2161 gggaagtgct tacgtgaaag agtcatttat taacaggtgg gcagtttcga tgatatgatc
     2221 ttttgaggtg catttccccc gggtggcggt gtcgatgtgt ttctggagct ggtcgcggct
     2281 ttctgtctgg gtgaggtcga gctccggctt caaccaccac tcgatcctgc gcccgcgccg
     2341 gaagaaggag actcacgatg aacttgccga ttgagaccga ggatatcggg aaacggtatg
     2401 gaaaggtcac tgcggtcaaa cacctctcct tgcgagtgga agagggagaa atctacgcgt
     2461 tcctgggctt gaacggcgcc gggaaaacga cgaccattcg tatgctgctg gggatgatca
     2521 aacccacctg gggaacggct cgggtgcttc aaacccgggt gcgcctggga agccgggaac
     2581 cctgggcagc ggtcggatac ctggtggaga caccgcacag ttatcaggag ttaaccgtct
     2641 tagagaacct ggaggtcgcc cgccgtttac atcctggcac tccgcccgag gccgtgggtc
     2701 gcatggtcga gcgcatgggg ctcgcggcct atgcggaccg gcaggctggt accctttctc
     2761 aggggaatgc gcagcggctg ggattggcca aagcgctgct gcatgctccc aggctcctgc
     2821 ttttagatga accggctaac ggcctggacc cggctgggat cgtcgagatc cgtgaacttt
     2881 tgctcgaact gacccgcgaa cagggtgtga cggtctttct ggcgagccat attctggccg
     2941 aagtgtcgcg gctggcccag cgcatcgggg ttctccacca ggggcgtctg ctccaggaac
     3001 tggacatcga tgagttggag cagaatcgac acaggcggct gctcttacga gcgcgggaca
     3061 tggaggcagc tcgcctggcg ctcaccactg ccggacatcc cgctgcaatg cttcgggacg
     3121 ggacgctcga actcactgat ctccgttcac ttgaacatcc tgacgacatc gctagcctgc
     3181 tggtgcaagc tggagcgccg cctacgcagg tgctggtcga ggaagaagac ctggagcagt
     3241 atttcctccg gttggttgga atgaatggag gacagaacca tgcatgacct gaccaatgcc
     3301 gtgtggattg aggtgcgcaa agccactcgt tctcggatgc cgctgtttac tgctctgggg
     3361 ttcctggtgc tggcttttgg cctcgccttt ttgatgttca tctataaaga ccccaccttc
     3421 gctcggagta gtggcctgat tagcgcgaag gccaacctgg caggaggggc tgccacctgg
     3481 ccgtactatc tgggtatcct cggccaggcg atcgcgattg gcggaatcct gttgtttagt
     3541 ctcgtggaga gctgggtgtt tggccgcgaa tttgtcgacg ggaccctgaa ggatctgctt
     3601 gccgtaccgg ttgcgcgtgc gaccattctc ctggcgaaat ttctcgttgt ggtgctctgg
     3661 tctctcctcc tgaccgtgat gctcttcctc gtgagcttgc tgctcggggc agcgattgga
     3721 ttgtcacagg ggacgacgga ggtgttctgg catggaaccg cgaccctggc agtcgccgcc
     3781 tgcctggtca tcgtcgatgt ttttccgttc gctttcttcg ccagtgtggg acgaggctac
     3841 ttgctgccaa tgggggtaac gttcctggtc cttgtggttg gtaatgtggt tgctattgcc
     3901 gggtggggta actatttccc ctgggcggtg ccagcgttgt atgcccagtt gaccggaaag
     3961 gggaatctcg atgcagccag ctacctgctt gtgctcctca cggggctcgc aggcatggcc
     4021 gggacctatc tctggtggag atttgcagat caaagccgct agccatcttg agttagaaag
     4081 agcaggctac ccgagctgct caagttccgc agcgccgaca tcaaacgagg ctgaagcgat
     4141 tattgagagc gtgtttggca atgtgctgcg gtagtccttc gtggcgagaa gagcaagtat
     4201 gatatcatga agatatcaga gggtagatcc acaaaaggag gttacactat gcgttacatc
     4261 atccgtgaga agtttttcca tctcggtgaa
//
LOCUS       DASZAK010000504         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_12476, whole genome shotgun sequence.
ACCESSION   DASZAK010000504 DASZAK010000000
VERSION     DASZAK010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12476"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VGT44_08885"
     CDS             <1..599
                     /locus_tag="VGT44_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2580954.1"
                     /translation="DIMLPEMDGLEVCRQLRREGPTSTTPIMMLTAKGDEIDKVVGLE
                     VGADDYVTKPFGRRELLARVRALLRRSDYPPAHDDHAMQDGSARGGEPNQSYRPDREL
                     VAGPIRIDLAGRRVNCRGKELELQPKQFDLLVYLARNRGTVLTRDQLLHNVWGYDYAG
                     DTRTVDVHVRWLREKIEEDPANPKLIQTVRGVGYCFRW"
     gene            674..1540
                     /locus_tag="VGT44_08890"
     CDS             674..1540
                     /locus_tag="VGT44_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV2580955.1"
                     /translation="MILSTTNLILSPDAAIDLHMHTTFSDGRWPAEQLIEYLRSEQFD
                     MVAITDHDRVDTMPVIQEIALQKQLPVLVAVEMTTQWNGIMGDVLCYGFDPQSAELRE
                     VADDVVRRQLENTLAVYAELLRKGYEFPKQAEVLHESGGELRLPRDNIMLLRAHGYAS
                     DWHSGLKILQESGFVSVKADMAHTVDTVHRCGGVALIAHPGRRERGFTFYDTELLDNV
                     RADVPLDGIEVVHPSHSPEVTESLLAYAKKHNLLTSTGSDSHSIPHRMPKKHRAELSR
                     ALLERVGIRVAA"
BASE COUNT          345 a          516 c          449 g          326 t
ORIGIN      
        1 tcgatatcat gcttcccgaa atggatgggc tggaggtctg tcgccagctg cgccgcgaag
       61 ggccgacttc caccacgccg atcatgatgc tcaccgccaa aggcgatgaa atcgataaag
      121 ttgtcgggct ggaggtcggc gcggacgact atgtcaccaa accctttggc cggcgagagc
      181 tgctggcgcg tgtgcgcgct ctgctgcgcc gttccgacta tccacccgca cacgacgacc
      241 atgccatgca ggatggcagc gctcgcggcg gcgagccaaa ccagtcctac cgccccgatc
      301 gcgagctggt cgccgggcca atccgtattg acctggccgg gcggcgcgtc aattgccggg
      361 gcaaagagct tgaattgcag cccaaacaat tcgatttgct ggtctacctg gcgcgcaacc
      421 gtggaacggt cctcacgcgc gaccagctcc tccataacgt ctggggctac gactatgccg
      481 gcgatacacg caccgttgac gtgcatgtgc gctggctgcg cgagaagatc gaagaagacc
      541 ccgccaaccc caaactgatc cagaccgtgc gtggcgttgg ctattgtttc cgctggtaga
      601 ccttaaccat gtggtatgat aggtacatct atcacatagt tccatccctt gatggttaag
      661 gagcataccc gttttgattc tttcgaccac gaacctgatc ctgtcccccg atgccgccat
      721 agacctgcac atgcatacca cctttagcga tggacgctgg cccgccgaac aattgattga
      781 gtatctgcgc tccgaacaat ttgacatggt ggccatcacc gaccacgacc gcgtcgatac
      841 catgcccgtc atccaggaga ttgccctaca gaaacagttg cccgtcctgg tcgccgttga
      901 gatgaccacg cagtggaacg gcatcatggg cgatgtcctg tgctatggct ttgaccctca
      961 aagcgccgaa ctgcgcgaag tggccgacga cgtagtgcgt cggcaattag aaaacaccct
     1021 ggccgtttat gcagaactgc tgcgcaaagg ctatgaattc cccaaacagg ccgaggtatt
     1081 gcacgaaagc ggcggcgaat tgcgcctgcc gcgcgataac atcatgctgc tgcgcgcgca
     1141 cggctacgcc tccgactggc attccggcct gaaaatcctg caagaatccg gttttgtctc
     1201 cgtcaaagcg gatatggccc atacggtaga caccgtccat cgctgcggcg gcgtcgcgct
     1261 gatcgcccac cccggtcgcc gcgagcgcgg cttcaccttt tacgataccg aactgctcga
     1321 taacgtgcgc gctgacgtgc cgctggatgg catcgaggtc gttcatcctt cccacagccc
     1381 cgaagtcact gagtcgctgc tggcctacgc caaaaagcac aacctgctca cgagtaccgg
     1441 ttccgactcc catagtattc cccatcgtat gccaaagaag catcgcgccg aattgagccg
     1501 cgcgctgtta gagcgcgtgg gaattcgcgt agcggcatga ggatcgatca aataaaaaca
     1561 accgtcccgg cgcgccggga cggttgtttt tatttgatcg atcctcatgc cgctacgcga
     1621 attcccacgc gctcta
//
LOCUS       DASZAK010000505         1855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_84063, whole genome shotgun sequence.
ACCESSION   DASZAK010000505 DASZAK010000000
VERSION     DASZAK010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84063"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..915
                     /locus_tag="VGT44_08895"
     CDS             262..915
                     /locus_tag="VGT44_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2580956.1"
                     /translation="MEVSWFGHSCFALRGKNVTLITDPFSPQPDHPQGETHRLGKINA
                     PIVTISHNHPGHNYAEGVGGNPRVVRGPGEYEISDVLITGVPSFHDSKRGAEAGRNTI
                     YIIHMDDLVICHLGDLGHTLQEEQLEEVADADLLLIPIAGQHTLNAAQAAEVISQVEP
                     RLVIPMHYRPSSDETPNPLDKFCREMGVEAVYPQPKLVVNRGTLPAAMQIVVLSTRG"
     gene            924..>1855
                     /locus_tag="VGT44_08900"
     CDS             924..>1855
                     /locus_tag="VGT44_08900"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV2580957.1"
                     /translation="MSKFIFVTGGVASSVGKGITVASLGRLLKNRGVSVSLMKLDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTDEPAYQANNVTTGQVYHAVIGKE
                     RRGEYLGGTIQVIPHITNEIKERIFSVARHSDADVVIVEVGGTVGDIEGEPFLEAIRQ
                     MRKDVGRRDVLYLHVTLLPYLMATKELKTKPTQHSVKELLRVGIQPDVILCRSDYPLG
                     DDLREKIALFCNIEERAVVPLLTTETIYEVPLILEEAGLGEYLVEELGLPKHQPHMEE
                     WRELVARIKRPRPELTIGLVGKYVELHDAYLSVA"
BASE COUNT          420 a          563 c          502 g          370 t
ORIGIN      
        1 gttgaagctg cccccgtgcg cgtcggcgcg cgcaaccgcg aagtcaacgt cacggtcgcc
       61 ttcagctccc tgaccttctc cgcccacggc accggcggcc agatgaaggt gagcggcccc
      121 ctgttggaag aagccgcctt aatcatgaat tcgacggaat aaatcgcctc ttgtagatca
      181 cccatccgtg tggtataata gcattcgggc gaggacagca gtccccgccc gactttatga
      241 gaaaagcgaa tttataaccg catggaagtg tcctggttcg gccactcgtg cttcgcgctg
      301 cgtggcaaaa acgttacctt aatcaccgat cctttttctc ctcagccaga ccacccgcaa
      361 ggtgaaacgc acaggctggg caagatcaac gcccccatcg tcaccatcag ccataaccac
      421 cctgggcata actatgccga aggtgtggga ggcaatcccc gtgttgtgcg cgggccaggc
      481 gaatacgaga tcagtgatgt cttgattacc ggcgtcccat ccttccacga tagcaaacgt
      541 ggcgcggaag cggggcgcaa taccatttat atcattcaca tggatgacct ggtgatctgt
      601 cacctgggcg acctcggcca cacgctgcaa gaagagcagc tcgaagaagt ggccgatgcc
      661 gatctgctgt tgatccccat cgccggacaa cacaccctca acgcggcgca ggccgctgaa
      721 gtgataagcc aggttgaacc gcgtctcgtc attcctatgc attatcgtcc ttcgagcgac
      781 gaaacaccca atccgctcga taaattctgc cgggaaatgg gcgtcgaagc ggtctatcca
      841 caacccaaac ttgtcgtcaa tcgcggtact ttacctgcgg caatgcagat cgtggtcctt
      901 tcgacgcgcg gctaggaggt ccaatgtcga aattcatttt tgttactggc ggtgtggcat
      961 catcagtagg gaaaggcatt accgtcgcct ccctgggtcg cctgctcaag aatcgcggtg
     1021 tctccgtctc cctcatgaaa ctcgacccat atatcaacgt cgatcctggc actatgtcgc
     1081 cctatcaaca cggcgaggtg ttcgtgaccg acgatggggc agagaccgac cttgatcttg
     1141 ggcattacga gcgctttacc gatgaaccgg cctaccaggc aaataatgtc acgacgggac
     1201 aggtctacca cgctgtaatc ggcaaggaac ggcgcggcga atacctcggc ggcacgatcc
     1261 aggtcatccc gcatatcacc aacgagatca aagagcgcat tttcagtgtg gcgcgccact
     1321 ccgacgccga tgtcgtaatc gtcgaggtgg gaggcaccgt cggcgacatc gagggcgagc
     1381 cgttcctgga agccattcgc cagatgcgca aagacgtcgg acgccgcgat gttctgtatc
     1441 tgcacgtcac gctgctgccg tacttgatgg ccaccaaaga gctgaaaacc aagcccaccc
     1501 agcacagcgt caaagagttg ctgcgcgttg gcattcaacc cgatgtgatc ctgtgtcgct
     1561 cggattatcc attgggcgac gacctgcgcg agaaaatcgc cctcttctgc aatatcgaag
     1621 aacgcgctgt cgtgcctctg cttaccactg agaccatcta cgaagtgccc ttgatccttg
     1681 aagaggctgg attgggcgaa tacctggtcg aagaactggg tctgcccaaa caccagccgc
     1741 acatggaaga gtggcgcgaa ctggtcgcgc gcatcaagcg gccccgcccg gaactgacga
     1801 tcggactggt cggcaaatac gtcgaactgc acgatgccta cctctccgtc gcgga
//
LOCUS       DASZAK010000506         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_617277, whole genome shotgun sequence.
ACCESSION   DASZAK010000506 DASZAK010000000
VERSION     DASZAK010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617277"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1285
                     /locus_tag="VGT44_08905"
     CDS             23..1285
                     /locus_tag="VGT44_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580958.1"
                     /translation="MDAQWIAVQGKDALNAVGIGAFFNLMNFGQMFGFHVLILPLLLV
                     FLVGLHLLQVRIRGVVRPFASTREEERARERLWRGAKRRKAAAGAGEPRPLPSDQARY
                     YRGLRMMRYDLVREGLIALLAVFVLTTVLAAVLSSADEPPLTLQQYAQQNPVGFVTTA
                     MGGLSGTGAVADYGPPYNSGTGSVQSIGPISLQQAAGVTIPIDAARVYVLDPLRIAAQ
                     TDPQLAAALQTFTHASAQQQTAWETAYTDALGKATVSNGHVVLAQGDYGSLPAMMDHL
                     LTLGTSGALDGLLLRSGGFYQNDFTRPLLFLSEDALPTRAEQLNMLGSQWGMMNSTGN
                     YPGQAWLWLYTFWYQIPPYNTSANADVQVWLTMAVLTLLLVVFPYLPYLNRLPYYLGV
                     HRLIWREYYRDERAAQAAGGTGLAAESG"
     gene            1398..1778
                     /locus_tag="VGT44_08910"
     CDS             1398..1778
                     /locus_tag="VGT44_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580959.1"
                     /translation="MSLTLIDSRQVEVRESDYDVDTHIQVRKGDTLMFNAWGQIWAGV
                     WLTGMNGPNGWNNLDPDPKFPLPGTHPYSLLGKLDNGYFFVGGYDRIDQTADQGELFL
                     RINDDKPANGSGAFECLVQVYRNE"
     gene            complement(2197..3042)
                     /locus_tag="VGT44_08915"
     CDS             complement(2197..3042)
                     /locus_tag="VGT44_08915"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEV2580960.1"
                     /translation="MSLEEPHGSHEVTDDYEEHDAQDTHDMHHARDFHTQSRRIASIT
                     AFLIVIALVAGLFSYYSIRRTGEGGTPTPSRTVVPTPTPTLIVPTPTQALFYETFIDN
                     RNIWALSNRGGFVREMANGALLLTNTNPLSTLIESIPSETSYDNFTMTITFAFDEGDA
                     NDSIGLYIRGDSNLDHDYRIEMSGDSTFDIAKEYLDTQSLPQVLTLDGPERISELRPQ
                     GQLNTMIVTAQGPTIRVTLNNTLISSVTDNDYASGQIALFVRHGATSLEATMSVSAIE
                     IDGQS"
     gene            3155..>3535
                     /locus_tag="VGT44_08920"
     CDS             3155..>3535
                     /locus_tag="VGT44_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV2580961.1"
                     /translation="MLAAMYYAPMDIRLEERPLPRPADGEVLLQVAAATTCGTDVKSY
                     RRGHPMLFRQTPAPFGHEAAGLVAATGKGVTSFVEGDAVVVANSAPCLQCFYCQRQRY
                     SLCENLLLLNGAYAEYLLVPERIVQ"
BASE COUNT          750 a          971 c         1010 g          804 t
ORIGIN      
        1 cggctacctg tcccagacca acttggatgc acagtggatc gccgtccagg gcaaggatgc
       61 cctgaacgcc gtcggtatcg gcgcgttttt caacctgatg aactttgggc agatgtttgg
      121 cttccacgtc ctcatcctgc cgctgctgct ggtgttcctg gtgggcctgc acctgctaca
      181 ggtccggatc cggggagtcg tgcgtccctt tgcctcgact cgcgaagagg agcgtgccag
      241 ggagaggctc tggcggggcg ccaagcgcag gaaagctgct gcgggagccg gtgagccacg
      301 accgctgccc tccgaccagg cgcgctacta tcgggggctg cgcatgatgc gctacgatct
      361 cgtccgcgag ggattgattg ccctgctcgc cgtcttcgtc ctcaccaccg tgctcgcggc
      421 cgtgctgtcc tcggccgacg agccaccgct gacgctccag cagtacgccc agcagaatcc
      481 ggtgggtttc gtgaccaccg ccatgggcgg gctgagcggc acgggagccg ttgccgacta
      541 tgggccgcct tataacagtg gaactggctc ggtccaatcg atcgggccga tttcgctgca
      601 gcaagccgca ggcgtgacca tccccatcga tgcagcacgg gtctatgtgc ttgatccctt
      661 acgcatcgcc gcgcagaccg acccgcaatt ggccgcggcg ctccagacgt ttacgcacgc
      721 cagcgcccag cagcagacgg cctgggagac cgcctatacc gatgcgctgg ggaaggcgac
      781 ggtgagcaat ggtcacgtgg tcctggcgca aggcgattac ggctcgctgc cggcgatgat
      841 ggatcatctg ctgaccttgg gcaccagcgg tgcgctggac ggactgctcc tgcgcagcgg
      901 gggcttctat cagaacgact ttaccaggcc actgctcttc ttgtcggagg atgcgctgcc
      961 gaccagggcg gagcaactga acatgctcgg ctcgcagtgg ggcatgatga actcgacggg
     1021 caactatccc gggcaggcgt ggctctggct ctacacgttc tggtaccaga tcccgcccta
     1081 caacacttcg gccaatgccg atgtgcaggt ctggctcacc atggcggtgc tgacactgct
     1141 gctcgtggtg tttccctacc tgccctacct caatcgcttg ccctattatc tcggtgtcca
     1201 tcgcctgatc tggcgtgagt actatcggga tgaacgggcc gcgcaggcgg ctggcggcac
     1261 gggactcgcg gcggagagcg ggtgaacctg ttgttgaaac ttgtagagaa acgtagagaa
     1321 gtcgtaagta gttcccatag ctgacccgag tccttgcttg tgattgtcag ccaggactgt
     1381 acaaaaggag aaaacaaatg tctctcacac tgatcgactc gcgacaagta gaagtacgtg
     1441 agtccgatta tgacgtcgac acccatatcc aggttcgaaa aggtgacacc ttgatgttta
     1501 atgcatgggg tcagatctgg gcgggagtgt ggctcaccgg catgaacgga ccaaatggct
     1561 ggaacaacct cgatcccgac ccgaaatttc ccttgcctgg cacccaccct tacagcttac
     1621 tgggtaagct cgataacggg tacttcttcg taggaggata cgacaggatc gatcagactg
     1681 ctgatcaagg cgagctcttc ttgaggatca acgacgataa gcccgccaac ggcagcggtg
     1741 cttttgagtg cctagtccag gtgtacagaa atgagtagag cagttgttta gtgtacttgg
     1801 ggtccgagta tgatccgact ttgttgtttt ccagtcggat catactcgga cccccacatt
     1861 gaccgcagca gaaaagaaaa tgggtttggc actcgcgtgg ggactaagaa ggctttgcca
     1921 acttatctta caaggcatac gtatcacacg ctacggtatt ttcgatcgca tccgattcct
     1981 cggactaacg gttggtattg agaatctgtc accacatgat ctgcgtcatt actcaacaac
     2041 cgacatatcg atattaagtt ctgatatcga tatgatccag cgctatagcg gatggaatag
     2101 ccctgttttg ccactatatt atgataaata gtccggagcc gactttgaaa gccttgaatt
     2161 tcaggcaaat acgagtttgt agaaactgcc attccttcaa ctctgcccat cgatctcaat
     2221 ggcgctgaca gacatcgtcg cttctagcga tgtcgcgccg tgccgcacaa agagcgcgat
     2281 ctggccgctt gcatagtcgt tatcggtgac agagctgatg agcgtattgt tgagtgtcac
     2341 tctgatggtg gggccttgag cagtcacaat catcgtattg agctgtccct gcgggcgcaa
     2401 ctcagaaata cgttccgggc catccagcgt caatacctgt ggcaaagatt gagtgtccag
     2461 gtattctttg gcgatgtcga aggtactatc accgctcatc tcgatgcgat aatcgtgatc
     2521 gagattacta tcgccgcgaa tatacaaacc gatactgtcg tttgcatcac cctcgtcaaa
     2581 cgcaaaggtt atcgtcatgg taaaattatc gtagctggtt tcgcttggta tgctttcgat
     2641 caatgttgag agcggattgg tattggtcag cagcagtgcg ccattagcca tctcgcggac
     2701 gaatccaccc cggttcgaca gcgcccagat attgcggtta tcgataaaag tttcatagaa
     2761 aagcgcctgc gtgggcgtcg gcacgatcag ggtgggtgtg ggcgtcggaa ccacagttct
     2821 actcggtgtg ggtgtccccc cctcacccgt tctcctgata ctatagtaag aaaacaaacc
     2881 cgcgaccaga gctatgacta taagaaaagc cgtaatgctg gcgattctcc tggattgggt
     2941 atgaaagtcg cgggcatgat gcatatcatg tgtatcctgg gcatcatgct cttcgtagtc
     3001 gtccgtgact tcatggcttc catgcggttc ctcaagcgac atgactggct cctgatgtgc
     3061 gaaaataagg atagttggcc gggtctgtgc ctgtcttctg gatgagtgtc taactcttca
     3121 acgatttatg agcaaacgag gtagcaaaat tcacatgctg gcagccatgt attatgcccc
     3181 gatggatatt cgactagaag aacgcccgct tcctcgccct gccgatggcg aagtgctgct
     3241 gcaagtggcg gcggcaacaa cctgcggcac ggatgtgaaa tcgtaccggc gcgggcatcc
     3301 gatgttgttt cgacaaaccc cggctccctt tggtcacgag gcggctggcc tggtcgcagc
     3361 aaccggcaaa ggagtaacga gtttcgtcga aggagacgcg gtcgtagtgg ccaattcagc
     3421 gccatgcctg caatgctttt attgccagag gcagcgctac agcctgtgcg aaaacctgct
     3481 cttgcttaac ggcgcgtacg cggaatacct gctggttcct gagcgcatcg tgcag
//
LOCUS       DASZAK010000507         3288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_687716, whole genome shotgun sequence.
ACCESSION   DASZAK010000507 DASZAK010000000
VERSION     DASZAK010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687716"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VGT44_08925"
     CDS             complement(<1..889)
                     /locus_tag="VGT44_08925"
                     /inference="COORDINATES: protein motif:HMM:NF024412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2580962.1"
                     /translation="MPKAQRARRERTDNYHLLREWCRTPEQRLYEEIRPITLFGVPPA
                     ERAQETGLAESTLRRAAAAFDTHGMISLFRPTKAQREDHHRSLPVPMRQLIVDLKAEY
                     PDFTLGEIASICAIQFDGRRPSKHTVKEVLADGPAPSRTTRQFLRYEEISEPEERRLA
                     VIRLHAQGWSISTIARYLEVSRPTIYATLKRWVEEGVRGLPDKSHANTSNPGVDLPTR
                     NLIRKKQEENRQLYGLHPLPKDSRKPKPHPFSATFRHERWCLDIRYMEKHRIPEIKGP
                     FYVITVMDAFSRAILSSAIF"
     gene            complement(976..1860)
                     /locus_tag="VGT44_08930"
     CDS             complement(976..1860)
                     /locus_tag="VGT44_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2580963.1"
                     /translation="MNTMNPNQRLVQSGRVTTEGDDLYYEVRGQGQPLLMIPPARGDG
                     DYYAAVADRLCDEYKVITYDRRANARSTMNNPQNFEISQQSRDAVAVLRAVGETSAFV
                     FGNSSGAVIALDMAKTQPQVVRAVVVHEAPVPRVLPQARKWQRHFAHVYVTALRFGSY
                     VAALQFVLGTQLPVRKMASAQTKANEYTRSRREQSGEHRLSNKVAADVLVKLELLPVS
                     NYLPDVELIKQNGVKVFIAVGEWALSRKTWYVQAAQILAEQLGCELVTFPGHHGSYMD
                     MPEEWATTLRRVLHKAEA"
     gene            complement(1862..2266)
                     /locus_tag="VGT44_08935"
     CDS             complement(1862..2266)
                     /locus_tag="VGT44_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580964.1"
                     /translation="MLVALANPLVSAMIKGQPDLFQGLLGAFDQKARKQLVGHADTYV
                     AQLQQAGLIRADLPVPVILYLMAALKMGVITAPDLIGEAHLPSMEQVAQALSDLLRRW
                     LELEQFPSDTTVGKQALGEWLKHFSNMEGQRE"
     gene            complement(2393..2590)
                     /locus_tag="VGT44_08940"
     CDS             complement(2393..2590)
                     /locus_tag="VGT44_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2580965.1"
                     /translation="MASSKTTHRTPGGFGPPDQPDERTLRAERLLDVAAALLVRWGYR
                     KTTLDDVAREAGVGKGTIYLH"
     gene            3093..>3288
                     /locus_tag="VGT44_08945"
     CDS             3093..>3288
                     /locus_tag="VGT44_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2580966.1"
                     /translation="MKTSEVPRAVAAGMSTASALGLLVDDAVVVHDSNRIAVRLLPCD
                     VLARVHYVAHQAGAKFEVEVA"
BASE COUNT          658 a          931 c          941 g          758 t
ORIGIN      
        1 gaaagatggc actggagaga atcgctcgcg agaatgcatc catgaccgtg atcacataaa
       61 aaggtccctt gatctctgga atgcgatgtt tctccatata gcgaatatcg aggcaccatc
      121 gctcatggcg aaacgttgcg ctaaatgggt gcggcttcgg cttgcgcgaa tctttcggta
      181 ggggatggag accatataat tgacgattct cctcttgttt cttgcggatc aggttgcgag
      241 tgggcaagtc aactcctggg ttactggtgt tcgcatgcga tttgtcagga agaccgcgca
      301 caccttcttc aacccatcgc ttgagggtgg cataaatcgt cggacgggac acctccagat
      361 accgagcgat ggtcgagata ctccagcctt gcgcatgcag gcggataacc gccaaacggc
      421 gttcttctgg ctcggagatc tcctcatagc gcaggaattg ccgggtcgtg cgcgacggcg
      481 ctggaccatc agctaagacc tccttaacag tatgtttact tggtcgtcgt ccatcaaact
      541 ggatggcaca aatactcgca atctctccca acgtaaaatc cggatactcg gccttgagat
      601 ccacgatgag ttggcgcatc gggacgggca acgaccgatg atgatcctct cgttgcgctt
      661 tggtcggacg aaagaggctg atcatgccgt gtgtatcaaa ggccgctgca gcccgacgga
      721 gcgtactttc cgccaaaccg gtttcttgcg cccgttctgc cggtgggact ccaaacaagg
      781 tgattggacg aatttcttcg tacagacgct gttcaggcgt tcgacaccac tctcggagca
      841 gatgatagtt gtcggtgcgt tctctccgtg ctcgttgggc ctttggcata tccgtcctcc
      901 tggatgcgca gcaaaggaga agagaaaccg cgtatccgtt tttcttccac cttttggact
      961 gttcgattca tcggtttacg cttccgcttt gtgcaagacc ctgcgcagtg tggtagccca
     1021 ttcctccggc atatccatat aggaaccatg atggccggga aacgtcacca gttcgcagcc
     1081 gagctgctcc gccaggattt gagccgcctg gacgtaccac gtctttctcg aaagcgccca
     1141 ttctccaacc gcgatgaaga ccttgacgcc gttttgcttg atcagctcga catccggcag
     1201 gtagttcgaa acgggcagca gctccagttt caccaggaca tcggcggcaa ctttattgct
     1261 gaggcggtgt tccccactct gctcccttcg gcttctggta tactcgttgg ccttggtttg
     1321 agcgctggcc attttgcgga cagggagttg ggtgccaagc acaaactgga gcgcggccac
     1381 atacgagcca aagcggaggg ccgtcacata gacgtgggca aagtgacgct gccatttcct
     1441 ggcctgcgga agcacccgtg gaaccggcgc ttcatgcacc acgactgccc gcaccacctg
     1501 cggctgggtt ttcgccatgt ccagcgcaat gaccgcgccg ctgctgttgc caaatacgaa
     1561 ggccgaggtc tcgccgaccg cacgcagcac ggccaccgcg tcccggctct gctgactgat
     1621 ctcgaaattc tgcggattgt tcatcgtact gcgggcattg gcacggcggt cgtatgtgat
     1681 gactttgtat tcatcacaga gccgatccgc cacggctgca taataatccc catccccgcg
     1741 ggcggggggg atcatcagca gcggctgtcc ctggccacgc acttcatagt acagatcgtc
     1801 gccttccgtc gtgacgcgac ccgactgcac aagacgttgg ttggggttca tagtgttcat
     1861 gttactcacg ttgtccctcc atgttgctga aatgcttcag ccactcgccc agggcttgtt
     1921 tgcctaccgt ggtatcgctg gggaactgtt caagctccag ccagcggcgg agcaggtcgc
     1981 tgagtgcctg agcgacctgc tccatggatg gcagatgggc ctctccgatc agatcaggcg
     2041 cggtgatgac gcccattttc agggcggcca tcagatacag aatgacggga accggaagat
     2101 cagcgcgaat cagcccggcc tgttgcagct gggccacgta ggtatcggca tggcccacca
     2161 gctgcttacg tgccttttgg tcaaaagcgc ccagcagccc ctggaagagg tctggctggc
     2221 ctttgatcat ggccgagacg agcggattgg cgagggcgac aagcatgcta tgcatccaca
     2281 ggcgatgcgg taacccaccc tctggatcgg cggcaatccg ctgcttgagg tcttcgctgg
     2341 cctgttggtt ggcgcgccag atggctgcgc gaaacaggtc gtttttatcc ttctagtgca
     2401 ggtagatggt gcctttgccc acgcctgcct cgcgggccac atcgtcaagg gtcgtcttgc
     2461 gatagcccca gcgcaccagc agcgcagcgg cgacatcgag aaggcgctct gcgcgaagcg
     2521 ttcgctcgtc aggctgatcg ggtggcccaa agcctcctgg cgttcgatga gtggtcttgc
     2581 tgcttgccat ggatcccttc atttctagcg gctcttgtgt gaccagctaa cttaatgact
     2641 caatgactca attaacaatt ctagtcacat attagatcaa caaatgtcaa aagtcaaagg
     2701 ctcccccgtg cgagacgcgc ggcctatggc caccaagtcc gcgtaggcgg actcctatcc
     2761 tcgcctggtg ccacggcgtt gatggcgaag agcaaccgcc tgaagtgcct ggagttgggg
     2821 gaatcggctc ctgaagggct ctgagtggtc ctagaaagct cgaaagctct tctattgtca
     2881 agaagtcagc ggctatttgt cagtgtcact gcacggattt gtaaaggatc cgtggcgagg
     2941 tttgatcgca gctacacagt agtgtgggta ggaagcacta tctcgttcat cggtgacatt
     3001 ctttttatga ctgtcctcac tttatggatt ggaacgctcc ttcataacca gagctatgca
     3061 ccactcgcca ctagtgggaa cggagggcaa cgatgaagac atcagaagtt ccgcgtgcgg
     3121 tggcggcggg tatgtccacg gcatcagcgc ttggcctctt ggtcgatgac gcagttgttg
     3181 ttcacgactc caatcgcatc gctgtgcgcc tactgccttg tgacgttctt gctcgggtac
     3241 actatgtagc gcatcaggcg ggcgccaagt tcgaggttga ggtcgctc
//
LOCUS       DASZAK010000508         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_188932, whole genome shotgun sequence.
ACCESSION   DASZAK010000508 DASZAK010000000
VERSION     DASZAK010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_188932"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1413
                     /gene="pstS"
                     /locus_tag="VGT44_08950"
     CDS             289..1413
                     /gene="pstS"
                     /locus_tag="VGT44_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV2580967.1"
                     /translation="MLKQSRLALVLGVALAISVLISACGSPTTTGGGGTPTASCPSVS
                     GLTGAGSTFDNPLFSQMFAASNYPTTKCGVNVNYQSIGSGSGINQLLAHTVDFGATDA
                     PLTDAQEAKSTTGNIIHVPVTLGAVAMSYNLSGVSGHLNLTPAIIAGMYLGTITSWDD
                     ASIKTANPGVTLPHEAISVVHRSDGSGTTSIFTHYLAAVSSDWSTKVGAATTVNWPVG
                     VGGKGSAGVAAAIKSTAGAIGYVELAYVLQNNLAYAAVQNVNGKLVLPSLDGAKADAA
                     NFTNIPPDLRFYIVNAAGDTSYPISGFSWVVVYQHQSNATKGEAVANLLWWMTHEGQQ
                     YATALDYVPLPSTIVTLDEGQIKSMTCGTSNTACYSGIVS"
     gene            1549..2523
                     /gene="pstC"
                     /locus_tag="VGT44_08955"
     CDS             1549..2523
                     /gene="pstC"
                     /locus_tag="VGT44_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV2580968.1"
                     /translation="MQQSLSGAKNVTRLLGGNGRRLSDTLFRGFTLLLVLVVVVVIGG
                     VVWVLVSNSLPTIQTFGLSFLSNQAFDPVHNVFGVAPAIFGTLVTSAIALIFAVPIGV
                     GTAIFLVDFCPRPLQKPLAFVVELLAAVPSVIYGLFGFLVLAAWLQSTGQPWLQKYLG
                     FLPFFQGQFHGLGLLAAGLILVIMVLPLITSVSREVILTTPTAQREALLALGATRWEV
                     IRHAVLPYSRIGITGGVILALGRALGETMAVTMVIGNQASPISASLFSTGYTLSSVIA
                     NQFTEATPGLFFSAVIEAGLLVFIITLSTNILARILIYRMGRVGGLVA"
BASE COUNT          518 a          739 c          710 g          607 t
ORIGIN      
        1 tcaaacgctg tttttcacgg tttaagccct accgttgatg atatcggcgg tggggctatt
       61 ttcttttttt tggcttctag agcgcgttta aacggatttg aagatcagga gaagatgaga
      121 aaagcctatc cccagggaat atttaaccgt tatttaacat atccataaag catagttaac
      181 ctgaccatgc taccgtatca gtggagaggg cgtcaggcgc atcagacctg ttgcccgcga
      241 aggaaaggaa tctacatcat tgctcgaaag agacgagcag gaggaattat gttgaagcaa
      301 tctcgcctgg cgcttgtgct gggagttgca ttagcgatca gtgtgcttat ttcggcctgt
      361 ggttccccca ctaccaccgg cggcggcgga acgcccaccg cgagctgccc ctcagtctca
      421 gggttgaccg gggcagggtc cacctttgat aacccgctgt tctcgcagat gttcgccgcg
      481 tccaactatc caacgaccaa gtgcggcgtc aacgtcaact accaatcgat cggcagcggc
      541 tcgggcatta accagttgct cgctcacaca gtggattttg gcgcgacgga tgcccctctg
      601 accgatgcgc aagaggccaa gagtacaacc ggcaacatca tccacgttcc ggtcactctg
      661 ggcgcggtag ctatgtccta taacctctct ggcgtttccg gacatctcaa tctgacgccg
      721 gccatcattg ccgggatgta tctcgggacg atcacctcct gggatgacgc ctcgatcaaa
      781 accgccaatc ctggcgtaac gcttccgcac gaggcgattt cagtcgttca ccgttctgac
      841 ggaagcggta cgacgagtat cttcacgcat tacctggctg ctgtgagcag cgactggtca
      901 accaaagttg gcgcagccac caccgtcaac tggccggtgg gcgttggcgg caaaggcagc
      961 gcaggcgttg ccgcggcaat caagagtact gccggggcca ttggctacgt cgaactggcg
     1021 tatgtcctcc agaataacct cgcttatgcc gctgtccaga atgtcaatgg gaaactcgtc
     1081 ctgccctcac ttgatggagc caaggcggat gcggccaatt tcacaaatat ccccccggat
     1141 ctgcgcttct atatcgtgaa tgcggctggc gacacttcct accccatctc gggcttctca
     1201 tgggtggtcg tataccagca tcagagcaat gccaccaaag gagaggccgt cgcgaatctg
     1261 ctctggtgga tgacacacga aggccagcag tacgccacag cgctcgacta tgtgccactg
     1321 ccctctacca ttgttaccct ggatgaggga cagataaagt caatgacctg cggcaccagc
     1381 aacacggcct gttactccgg gattgtcagc taatatgatt gcggaagacc tcttccgcag
     1441 cgtggcttcg tgtgtctgta gttcggcgaa tgcatcagag tagacgcagg cacacgaggc
     1501 tttcgcgcat gaagcccggt cctggtgcaa atatgaaagt ggtgttgtat gcaacagagc
     1561 ctttcagggg cgaagaatgt aacgcgcttg ctcggtggta atggtcgtcg gctcagcgat
     1621 acactgttcc gcgggttcac gctgctgctc gtgctggtgg tggtggtagt gattggagga
     1681 gtcgtctggg tacttgtcag taattcgctg ccgacgatcc agacctttgg tttgagtttt
     1741 ctctccaatc aggcctttga tcctgtccac aacgtgttcg gggtcgcccc tgccattttc
     1801 gggacgctcg tgacatctgc tatcgctctc attttcgccg tcccgattgg cgtaggcacg
     1861 gctatcttcc ttgtcgattt ctgcccgcgc cctctccaga aacccctggc attcgtcgta
     1921 gaactgctgg ccgccgtgcc aagcgtcatc tatggtctct tcggatttct cgtgctggca
     1981 gcctggttgc agtcaacggg tcaaccctgg ctgcaaaaat acctgggttt cctgcccttc
     2041 ttccaggggc agtttcacgg gctgggcctg ctggcagccg gtttgatcct ggtgataatg
     2101 gtgctgcccc tgattaccag cgtctcacgc gaggtcatct tgacgacacc aacagcgcag
     2161 cgtgaggcgc tcctggcgct gggcgcaaca cggtgggaag tcatccgcca cgcggtactc
     2221 ccctactcgc gcatcggcat caccggcggg gtgatcctgg cgctgggtcg cgcgctcggc
     2281 gaaacgatgg ccgtaacgat ggtgattggc aaccaggcat cgcccatcag tgccagcctc
     2341 ttcagtaccg gttatacact ctcgagtgtg attgcaaacc agttcaccga ggccaccccc
     2401 ggcctcttct tctcagcggt gatcgaggcc ggcctgctgg tatttatcat cacgctgtca
     2461 accaatattc tcgctcgtat actgatttac aggatgggtc gtgttggagg acttgtcgca
     2521 tgagtatcat agttgaaaag ccacaggctg ccaatccctt tcaggctcct cgta
//
LOCUS       DASZAK010000509         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_687765, whole genome shotgun sequence.
ACCESSION   DASZAK010000509 DASZAK010000000
VERSION     DASZAK010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687765"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VGT44_08960"
     CDS             <1..512
                     /locus_tag="VGT44_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV2580969.1"
                     /translation="PLDQIIEQLNVIGGANGIGKIDIFEDGIMGLKSREIYEAPAATI
                     LLKVHKDLEQYCLTKEEIQLKRQMDAQWARMVYHGEWFHPLKNAIDAFIAATQPVVNG
                     EYTLELYKGNIDIVSRESKSGLFFPDVRSIKAASFNQKESAPAAHLRGIPYELIARRD
                     KTAANVYGV"
     gene            522..1904
                     /gene="argH"
                     /locus_tag="VGT44_08965"
     CDS             522..1904
                     /gene="argH"
                     /locus_tag="VGT44_08965"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV2580970.1"
                     /translation="MTKLWQKSYQLHELVERYEAAQNSKLDERLIRHDVWGSLAHVAM
                     LAKIGVLSESEHRALKDALCRVLDMEAAHEFTIALTDEDVHTGIENYLSSVAGPAGKK
                     IHMARSRNDQVLVDMRLYAKEQLHQVVEKLGNLCHALLDFASKHEHVPMPGYTHMQRA
                     MLSSVGLWASSFAESLLDDEQLLQAAYALNDQSPLGSAAGYGVPVQIDREYSARLMGF
                     ARVQGNVIYAQNSRGKIEAAIVQALAQIMLDLSKMAQDILLFTTVEYGFFHPPQELCT
                     GSSIMPQKRNLGVMELVRARTQTMLALQQQILGIVTGLPSGYNMDYQETKRPFMEALD
                     LVQESLDICALVVSSLEVDTERLVAACTFELFAADRAYELTSTAKLPFRDAYRIVAAE
                     VTAQLDRKQPFPAESQEQLIERLKARSHTGGSGNLGLDRLYDRVETIEQTWNEKTKQF
                     EEAIAALVNT"
BASE COUNT          482 a          673 c          561 g          342 t
ORIGIN      
        1 tgccgctcga ccagatcatc gaacagttga acgtgatcgg cggcgcgaac ggcatcggca
       61 aaatcgacat cttcgaagac ggcatcatgg gtctcaagtc gcgcgaaatt tacgaagcgc
      121 ccgccgcgac catcctgctg aaagtccaca aagacctgga gcaatactgc ctgacgaagg
      181 aagagatcca attgaaacgc cagatggacg cgcagtgggc caggatggtc tatcacggcg
      241 aatggttcca cccgctcaag aacgccatcg acgcctttat cgccgcgacc cagccggtcg
      301 tcaacggcga atacacgctt gaactctaca agggcaacat cgacatcgtc tcgcgcgagt
      361 cgaaaagcgg cctcttcttc cccgacgtgc gttcgatcaa ggctgccagc tttaaccaga
      421 aggaatccgc tccagccgcc cacctgcgcg gcatccctta cgaattgatc gcgcgccgcg
      481 acaaaaccgc cgccaacgtg tacggcgtat aaggaaaaac catgaccaaa ctctggcaaa
      541 aaagctatca actccacgaa ctggtggagc gctacgaggc ggcgcagaac agcaagctgg
      601 acgagcgcct gatccgccat gacgtgtggg gatcgctcgc ccacgtcgcg atgctggcga
      661 agatcggcgt cctgtcagag tccgaacaca gggcattgaa agatgccctg tgccgcgtac
      721 tcgatatgga ggccgcccac gaattcacta tcgccctgac cgacgaagac gtgcataccg
      781 gcatcgaaaa ctacctgtca tcggtggcgg gacctgcggg caaaaaaatc cacatggcgc
      841 gctcgcgtaa cgaccaggtg ctggtggata tgcgcctgta cgccaaagag cagctgcacc
      901 aggtcgtcga aaagctggga aacctctgcc acgccctgct cgattttgcc agcaaacacg
      961 agcatgtccc gatgcccggc tacacgcaca tgcagcgcgc catgctctcc tccgtcggcc
     1021 tgtgggcatc cagcttcgcc gaatcgctgt tggacgacga gcagttgtta caggcggctt
     1081 atgccctcaa cgaccagtcg ccgcttggct cggctgctgg ttacggcgtc cccgtccaga
     1141 tcgaccgcga atactccgcg cgcttgatgg gcttcgcgcg cgtgcagggc aacgtcatct
     1201 acgcacagaa cagccggggc aagatcgagg ccgccatcgt gcaggcgctc gcgcaaatca
     1261 tgctcgacct gagcaagatg gcgcaggaca tcctgctctt caccaccgtc gagtacggct
     1321 tcttccaccc gccgcaggaa ctctgcaccg gcagcagcat catgccgcag aagcgcaacc
     1381 tgggcgtcat ggaactggtg cgcgcccgca cacagaccat gctggcgctg caacagcaaa
     1441 ttctcggcat cgtgaccggc ctgccttccg gctacaacat ggactaccag gagaccaagc
     1501 gccccttcat ggaagcgctc gacctcgtgc aggagagcct ggacatctgc gccctcgtcg
     1561 tctcctcgct cgaagtcgat accgagcgcc tggtcgccgc ctgcaccttt gaactcttcg
     1621 ccgccgaccg cgcctacgag ctgacctcga ccgcgaaact gcccttccgc gacgcctacc
     1681 gcatcgtagc cgccgaagtc accgcccaac tggaccgcaa acagcccttc ccggcagaga
     1741 gccaggagca actcatcgaa cgcctcaaag cccgcagcca caccggcggc tccggcaacc
     1801 tgggtctgga taggctatat gatagggtag agacaattga acaaacctgg aacgagaaaa
     1861 ccaagcaatt cgaggaggct atagcagccc tggttaacac gtaggtgccg atttatcgcg
     1921 cacaccgccg attcatcggc cacaatggac attaacggcg atcaatcggg ccgatgaatc
     1981 ggcggtgtgc gcgataaatc ggcaccttcg gatatatcca aacccgttat acaggaagga
     2041 ttcacaccta tggcaacc
//
LOCUS       DASZAK010000510         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_544942, whole genome shotgun sequence.
ACCESSION   DASZAK010000510 DASZAK010000000
VERSION     DASZAK010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544942"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1886
                     /locus_tag="VGT44_08970"
     CDS             57..1886
                     /locus_tag="VGT44_08970"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV2580971.1"
                     /translation="MSDTSLEQPTPTPSAGGNEPTEEGSFGVIEGASPAPVTPLTAPT
                     QVDPIPPAALKRANRSATIEHLAAIQKVTPLSDEDPDVKQKMSDLRTDLTTLLTDLRW
                     GGYSVKATVERLTPLLDVGPLEDWAPVLVPTILEIDRAGNLVPAWIKLVEEEDPQDIP
                     EDANPANTTIGRARRVALLMLGYYKTPELSQLLGKLASDPRSSLYATQSLVKQGTVAG
                     LQGLMAALKTARGWAKVDVIDTFASLNQARFHEIMLASGLNDAEGLESYIAPPLYRTV
                     QLENYLRGGEGVAPRLTQQATLVFAQVIQDHNPSATGSETPPVLFERDVASLVSALLD
                     GVRLTPDWRGAVALHRLGLLVGRYWGDISRGVAYDQDITQQIYACLPLMPAVEQWMNS
                     AGRVALLDGLANDESAFSPCLKALRDLREAQAVNTLLAQLDRVSSIQNREQASRIGQI
                     CDTLAQLYDPRTSATLQRLVQRTIPAQSRAARNRRSENLAVGDPDIPASIVYGASLRA
                     LASFQDRSALDLVLTATRDFDPYVRAQALEALKGIDPTGEDPRSREAVRDALNDPRDA
                     VARTACQLAGQYRDLEAMPILTRLAESRPQLGPSVQDALRRLG"
BASE COUNT          388 a          595 c          570 g          368 t
ORIGIN      
        1 gtatacacac gtatacatat gcaaccatat ttagtagaaa agggatagtg cataccatgt
       61 cagatacaag cctggaacaa cctacaccta ctccctcggc tggcgggaat gaaccaaccg
      121 aggaaggctc atttggtgtt atagagggtg ctagtccagc ccctgtgact cccctgactg
      181 cgccgactca ggttgatccg attccaccag ccgcgctgaa acgcgcgaac cgctcagcga
      241 ccattgagca tctcgcggcg atccagaagg tgacgccact cagcgatgaa gaccccgatg
      301 tcaagcagaa aatgagcgac ctgcgcacgg acctgaccac gttgctcacc gatctgcgct
      361 ggggtggata ttctgtcaag gcaaccgtcg aacggctcac tcccctgctg gatgttgggc
      421 cgcttgaaga ctgggcgccg gtgctggttc ccacgattct tgagatagac cgcgctggca
      481 accttgttcc ggcctggata aaactggttg aagaagagga cccgcaggat atcccggaag
      541 acgccaatcc tgccaatacc accatcgggc gggcgcgccg cgtggccttg ctgatgttgg
      601 ggtattataa gacgcccgaa ctttcgcagc tcctgggcaa gttagcctct gacccgcgtt
      661 cctcgcttta cgcgacacaa tcgctggtca agcaaggcac ggtggccggt ttacagggat
      721 tgatggccgc gctcaagacg gccaggggat gggcgaaggt ggatgtcatc gacacatttg
      781 ccagcctgaa ccaggcgcgt ttccacgaga ttatgctggc aagcggcctg aatgacgccg
      841 aaggattaga gagctatatt gctccgccgt tgtatcgcac ggtccagttg gagaactacc
      901 tgcgcggtgg cgaaggtgtc gccccgcgtc tcacgcagca ggcgacgctc gtctttgccc
      961 aggttattca ggatcacaac ccgtcggcaa ccggcagtga gacaccgccg gtcctttttg
     1021 aacgcgatgt ggcgtcgttg gtatcagcgc tgctcgatgg cgtccggctg acccccgact
     1081 ggcgcggcgc ggtggcgttg caccgcctgg gattgctggt gggccgctac tggggcgaca
     1141 tctcgcgtgg agtggcctat gaccaggaca ttactcagca gatctatgcc tgcctgccac
     1201 tcatgcctgc tgtcgagcag tggatgaaca gcgcggggcg cgttgctctg ctggatgggt
     1261 tagcgaacga tgaaagcgca tttagcccct gcctgaaggc gctgcgagac ttgcgcgagg
     1321 cgcaggctgt caacacgctg ctggcgcagc ttgaccgcgt ttcctctatt cagaaccgcg
     1381 aacaggcatc tcgcattggg caaatatgcg acacgcttgc acagttgtac gatccgcgca
     1441 cgagcgcgac actccaacgc ctggtacagc gtaccatccc agcgcaatcg cgagcagccc
     1501 gcaatcgacg cagcgagaat ctcgcagttg gcgaccccga cattcccgcg agcatcgtct
     1561 atggcgcatc cctacgcgcg ctcgcctcgt tccaggaccg cagcgccctc gatcttgtcc
     1621 tgaccgccac gcgagacttc gatccttatg tgcgagcgca agcgctcgag gcgttgaaag
     1681 gtatcgaccc caccggcgaa gacccgcgca gtcgtgaggc cgtgcgcgac gcgctcaacg
     1741 atccgcgcga cgctgtggcg cgtactgcct gccaacttgc cggtcagtat cgcgatctcg
     1801 aagccatgcc catcttgacg cgcctggccg agtcccgccc ccaactcggc ccatccgtcc
     1861 aggatgcctt gcgccgattg ggataacagg tagcgttgat acatggcgac cgcaagggtg
     1921 c
//
LOCUS       DASZAK010000511         2507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_296007, whole genome shotgun sequence.
ACCESSION   DASZAK010000511 DASZAK010000000
VERSION     DASZAK010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296007"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..392)
                     /gene="secG"
                     /locus_tag="VGT44_08975"
     CDS             complement(162..392)
                     /gene="secG"
                     /locus_tag="VGT44_08975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV2580972.1"
                     /translation="MTQWLPAIRVVQIVLSVSVIFFILLQARGAGLGSAFGSTSGAVF
                     KTRRGVERLIFNITIVFIILFSAISLLGTLIH"
     gene            complement(554..1078)
                     /locus_tag="VGT44_08980"
     CDS             complement(554..1078)
                     /locus_tag="VGT44_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580973.1"
                     /translation="MRVQEHLKLSTLAAVMTLPWLKQDALIPLVASIGIDIDHYLWHA
                     VTHRTLSLRAAVRYFGQADPPQLPQQRALHHPLLLAALLVLAVRLRSRLLLLILSGLL
                     FHVSLDAIHVTQMSHLKRSLSEQAGWQCPACGRHEEALQLHTTHFARNLLDRYNPRHF
                     VVLCPACHEQAHLP"
     gene            1343..>2507
                     /locus_tag="VGT44_08985"
     CDS             1343..>2507
                     /locus_tag="VGT44_08985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2580974.1"
                     /translation="MNIVTDTQVELANETDTIEDSIIECSVGIMAYNEEANIARTLEA
                     VLDQTGPSIRLHEVVVVASGCTDRTVPIVSEMALKDARIRLCVQEKREGKASAINLFL
                     KQATSPVVMLIGADVIPETSAIEQLCSPFLDPAIGMVGGRPVPINDPNTFMGHAVHLL
                     WRLHDRVARIHPKLGEVIAFRNVISGIPTDSAVDEISIQALITQLGYKLIYRPQCVVY
                     NKGPVTVRDFLKQRRRIYAGHLKVLRQQNYEASTMKVSPIFRQLIACRHFALNTPKKA
                     VWTMLAMALEAYARVQGHYDVWRKREHYIWSAVSSTKDLEAGQLKMRRVCNEQSVIVF
                     RLLLEGAKHNDVHREREEHEANEAARKLLPILRSKLRKEDALSINGPGIMTAVI"
BASE COUNT          627 a          710 c          667 g          503 t
ORIGIN      
        1 aggaggcgat agttcccaag ctgcggcaag ctgccatcgg gtaagacaag acgacccctc
       61 gcgcttagct gattcccaac acgcaccgtt ggaagctcgg aaggggatgc ctggtccata
      121 tcttgccctc aacgccccat cgtggagaca cgatgatgca tttagtgtat cagcgtacca
      181 agcagcgaga tggcggagaa aaggatgatg aaaacgatag taatattgaa aatcagccgt
      241 tctacgcctc gccgggtctt aaataccgcc cccgacgtac tgccgaatgc gctccccaga
      301 cctgccccac gcgcctggag caggataaag aaaatcactg agacgctgag tacgatctgg
      361 acgacgcgaa tcgctggaag ccattgagtc acaattttcc tccatagtct atcttcacct
      421 ggcgcttcac cagcaaacgc gtcggcccag cccagggaaa taacgtgcct tgtaccgaca
      481 gggattatat catagaatga ttgacccgtc gagagtatca acgaacaatc atcgccgcca
      541 gtcgcgcgtg gtcctatggc aaatgcgctt gttcgtgaca ggcggggcac aacacaacga
      601 agtggcgtgg attgtaacgg tcgagcagat tgcgcgcgaa atgcgtggta tgcagttgca
      661 acgcctcctc gtgcctgcca caggcgggac actgccaccc ggcctgctcg gaaagcgatc
      721 gtttgaggtg gctcatctgc gtcacatgga tagcatccag gctgacatgg aagagcagac
      781 ccgacaggat cagcagcagc agccgcgatc gcaggcgcac agccagcacg agcagcgctg
      841 ccagcagcag agggtgatgc agcgcgcgct gctgcggcag ttggggcggg tcggcctgcc
      901 caaaatagcg cacagcagcg cgcaaactca gggtgcgatg cgtcaccgcg tgccagagat
      961 aatggtcgat atcgatgcca atactggcca ccagggggat cagcgcatcc tgtttgagcc
     1021 agggcaaagt cataaccgcc gcgagtgtag agagcttgag atgttcttgc acacgcataa
     1081 catctccttt ccagaaaaca atgaaattca acgttcattt ttggtataag cagaaatata
     1141 gtcatgccaa tttccgccag ttaagtataa atcgcggggg agagacggtc aatcagagta
     1201 agccgtacaa attcataact gtatttgcat atttgcaacc atttctcaac cggttatcta
     1261 ttggcacata acgatgtgat gatattcttt accacatagt aaaatcctat aatgcctcac
     1321 acgagggaga ttattgaagg caatgaatat cgtcaccgat acacaggttg aactagccaa
     1381 cgaaactgat actatagagg acagcattat cgagtgcagc gtaggaataa tggcctataa
     1441 cgaagaagcg aacattgcgc gcacgctgga agctgtactc gatcaaaccg gcccctcgat
     1501 ccgcctgcat gaagtcgttg tggtcgccag cggttgcacc gaccgcaccg ttcccatcgt
     1561 ttccgaaatg gcgttgaaag atgctcgcat ccgtctttgt gtccaggaga agcgcgaagg
     1621 caaggccagc gccatcaacc tctttctcaa acaggcgacc agcccggttg tgatgctgat
     1681 cggagcggat gtcattccag aaacctcggc tatcgagcag ctttgctcgc cattcctgga
     1741 ccccgcaatt ggcatggtcg gtgggcgacc ggtgccgatc aacgacccga acacctttat
     1801 gggacatgct gtccacctgc tctggcggct gcacgaccgt gtcgcgcgca tccatcccaa
     1861 gctgggcgaa gtcatcgcct tccgcaacgt tatttcgggt atacccaccg acagtgccgt
     1921 cgacgaaata tccattcaag ccctgattac acaacttggc tataagctca tctaccggcc
     1981 tcaatgcgtt gtctacaaca aagggccggt cactgtgcgt gacttcctca agcagcgccg
     2041 ccgcatctat gccgggcatc tcaaggtatt gaggcagcag aactacgaag cctcgaccat
     2101 gaaggtcagc ccgattttcc ggcaattgat cgcctgccgt cacttcgccc tcaacacccc
     2161 gaagaaagcc gtgtggacca tgcttgcaat ggccctggaa gcctatgcgc gcgtgcaggg
     2221 gcattatgat gtctggcgca agcgcgagca ttatatctgg tctgcggtct cctctacgaa
     2281 agacctggag gccggtcagc tcaaaatgcg ccgcgtctgc aacgagcaaa gcgtgatcgt
     2341 cttccggctc ttacttgaag gcgcgaaaca taatgacgtg catcgcgaac gcgaagaaca
     2401 cgaagccaac gaggcggcac gcaagctgct gcccatcctg cgttccaaac tgcgcaaaga
     2461 ggacgcgctt tcgatcaacg gccccggcat tatgaccgcc gttattc
//
LOCUS       DASZAK010000512         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_403977, whole genome shotgun sequence.
ACCESSION   DASZAK010000512 DASZAK010000000
VERSION     DASZAK010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403977"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="VGT44_08990"
     CDS             complement(<1..779)
                     /locus_tag="VGT44_08990"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2580975.1"
                     /translation="MKTSRDKPHWQDRRRHVARGRMAWYGEPGDERYWLAYWKERLTP
                     DYYVRAEHIGLHDDELGSILLRYLPSRGLHLEAGCGVGYWVAALREKGMWIEGIEYAR
                     ELVELARSAHPGLPVRWGDALAIDRPDCTYDTYLSIGVVEHREAGPEPFLREAYRVLK
                     PGGRIIVAVPFYGPLRRIKQRLRLYEPRPSALPFFQYGFQQADMRRLLRQAGFYVEFA
                     RPLYAHRLLQEELRGYDRLSSRFDFIKGPAERLLNRRDGHML"
     gene            867..1643
                     /locus_tag="VGT44_08995"
     CDS             867..1643
                     /locus_tag="VGT44_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HEV2580976.1"
                     /translation="MQIRNSGVLITGGASGLGGACAELLAHDGAKVAIIDLNGEMGEA
                     LAAKLNAAGHTAIFVKTNVTDEESMQAAVRAAVDAFGSIHILINCAGISVAERVIGKN
                     GPSSLASFTRVIQVNLIGTFNAIRLAAAAMAGNEPNAGGERGVIINTASVAAFDGQIG
                     QAAYSASKGGIVGMTLPIARDLARHGIRVMTIAPGIFETPLLGNLPEPARISLGQQVP
                     FPPRLGQPPEYAALAKHIIENEMLNGEVIRLDGGIRMAPK"
     gene            2061..>2357
                     /locus_tag="VGT44_09000"
     CDS             2061..>2357
                     /locus_tag="VGT44_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEV2580977.1"
                     /translation="MAGSAFETNPVYLKKLLDNCQSGILQLPDFQRSWVWDEDRIKSL
                     IASISRAFPIGALMALAAGGSVNFKPRPVEGVPIEAKQVTPNSLLLDGQQRLTSL"
BASE COUNT          552 a          684 c          639 g          482 t
ORIGIN      
        1 agcatatgtc catcccttct attaaggaga cgttctgccg gcccttttat aaaatcaaag
       61 cgcgaggaaa gccgatcata gccccgcaac tcctcttgca acaggcgatg cgcgtacaat
      121 ggccgggcaa actccacgta aaatccggcc tggcgcagca ggcggcgcat atccgcttgc
      181 tggaaaccat actggaagaa gggcagcgcg gatggtcttg gttcgtacag gcgcaggcgc
      241 tgctttatcc ggcgtagcgg gccatagaac ggcacggcca cgataatcct gccgcccggc
      301 ttcaacacgc gataggcttc cctcagaaac ggctcaggcc ccgcttcgcg atgttctacc
      361 acgccgatag acaggtatgt atcataggta caatcaggcc ggtcgatagc cagggcgtca
      421 ccccatcgca ccggcaagcc cggatgtgcc gatcgcgcca gttcgaccag ttcgcgcgcg
      481 tattcgatgc cttcgatcca catccccttc tcgcgcaggg ccgcaaccca gtaacctacg
      541 ccgcagcccg cctcaaggtg cagcccgcgc gacggcagat agcgcagtag gatgctcccc
      601 aactcatcgt catgcagccc gatatgctcc gcccggacgt aataatcagg cgtgaggcgc
      661 tccttccagt acgccaacca gtaacgctcg tcgcccggct cgccatacca cgccatgcgc
      721 ccgcgcgcca cgtgcctgcg ccgatcttgc cagtggggtt tatcacgcga agttttcatg
      781 ctacgacgct cacggtgttg tagaataaat atcatcagta atgtgaataa accattctat
      841 catgctaact tcggaaagga acccacatgc agatcagaaa cagcggtgtt ctcataacgg
      901 gtggcgcgtc gggcctgggg ggcgcgtgcg ctgaactgct ggcgcacgac ggggcgaagg
      961 tcgccattat cgacctgaac ggcgagatgg gcgaggcgct ggccgcgaaa ctgaacgccg
     1021 cagggcatac cgccatattt gtcaagacca acgtcacgga tgaagagtcg atgcaggccg
     1081 cagtgcgcgc ggcggtcgat gccttcggct cgatccatat tttaatcaac tgcgcgggca
     1141 tcagtgtggc tgaacgggtt atcggaaaga acggtccgag cagcctggcc tccttcaccc
     1201 gcgtgatcca ggtcaacctg atcggcacct tcaacgccat ccgcctcgcg gctgctgcca
     1261 tggccggaaa cgagccgaac gcgggcggcg agcgcggcgt catcatcaac accgcatccg
     1321 tcgccgcctt cgatggacag atcggccagg cggcctattc cgcctcgaag ggcggcatcg
     1381 tgggcatgac gctgcccatt gcccgcgacc ttgcccgcca cggcattcgc gtcatgacca
     1441 tcgctcccgg catcttcgag acgcccctgc tgggcaacct gcccgaaccc gcgcgcattt
     1501 cgctcggcca gcaggtgcca ttcccgccgc gcctgggcca accgcccgaa tacgcagccc
     1561 tggcaaagca catcatcgaa aacgagatgc tgaacggcga ggttatcaga ctggatggcg
     1621 ggattcgcat ggcccctaaa taatctcttc catatagccg atgagaggga accgtcagta
     1681 tgctaatgga ttcctctcat tgtcgaggct atgaagtggc ttctaagttg cgtgataaat
     1741 gtgctgatta cctctagtat tgtctaagga agtggggtag cttaaaagga gcatagtatc
     1801 acaaaggcta ttcagaagac ggttttactg ataaaattct atagaaggcg gcagcaaagc
     1861 taatagtcgg tacgataatc tgtgactttt ggtccaaccc atattacgaa aatacaaata
     1921 tccgcccgat ttagagcaac gcgccattga tttagtgctt cgacaggcgg aaaccttatc
     1981 cgaggcctgg atatagcgaa acagcaaaac atatatgatt gagagactgt acagcacatg
     2041 ataaaggaga acagaaaata atggccggat cggcatttga gacgaatcct gtttatctga
     2101 aaaaacttct cgacaactgt cagagcggta ttcttcagtt gccggatttc cagcgcagtt
     2161 gggtttggga tgaagaccgc atcaaaagct tgattgcttc aatctcgcgt gcttttccta
     2221 ttggcgcatt gatggcgctt gcagcgggcg gatcggtaaa ttttaagccc agacctgttg
     2281 aaggtgtgcc aattgaggca aagcaagtta ctcctaactc tctactactt gatggccagc
     2341 aacgattgac ctcgctt
//
LOCUS       DASZAK010000513         3001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_404015, whole genome shotgun sequence.
ACCESSION   DASZAK010000513 DASZAK010000000
VERSION     DASZAK010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404015"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /locus_tag="VGT44_09005"
     CDS             complement(<1..1117)
                     /locus_tag="VGT44_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV2580978.1"
                     /translation="MTTTSTVELIPRDILFGNPEKSSPQVSPDGTRMAYIAPVNTVLN
                     VWVGTIGQDDYRPVTQDTERGVRFYAWAADNRHILYIQDIKGDENWRLYATDLQSGET
                     RDLTPFERVQARVVARDKHFPNELLVGLNKDNPQSHDVYHLDLATGELALVTQNPGNI
                     LAWVVDAHLKVRGAMAAAPDGSGAMELLVRANEDEQWSTLLTWDADDNQSSGPAGFTL
                     DGQALYLQDSRNVNAGRLVKLEIATGNTTVIAQDPDYDASSVMMHPDTREVQAVAFVK
                     DRLEWVVLDESIRADFDYIRTLHRGDFAVSSRDHADKTWIIAFTTDDGPVSYYAYDRA
                     TRSATYLFDNQPALKNYTLSTMQPFTFAARDGLTIHGY"
     gene            complement(1114..1293)
                     /locus_tag="VGT44_09010"
     CDS             complement(1114..1293)
                     /locus_tag="VGT44_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580979.1"
                     /translation="MDNQQQSQLLKGFGSVTPLHRPEDLETLRVAFEQAVADEVMEEM
                     KAIDTLHKTDTKEQA"
     gene            1786..>3001
                     /locus_tag="VGT44_09015"
     CDS             1786..>3001
                     /locus_tag="VGT44_09015"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2580980.1"
                     /translation="MNPEPGTDGRPAPAPNGHYESMVASDGIVYVGSDNGSLYALNGS
                     DGRVLWQYMANAPVSVVAVVDGAVYAYADNPNSAVAFALNAGSGNVVWRYQVSDFVSK
                     AMVVDKMVYLGTAATANQPVLYALQTSRGALAWRYAAHAITPGLLAVSDGVVYYAEIS
                     GIGTAFTENITALGAGDGRALWRLRTQSADGYAYAVSAVVNGAVYISTNTGAVSAARA
                     SDGTLLWHSARASIFPGVPSAATPLVADGMVYVTGKQNFASRSQGLDALRASDGKQLW
                     TQAIDGGPGPQVGQPQLVDGVLYYSGSSGVIALRASDGARLWVYSGDTPAGPFFVSDG
                     RVYANSAGGLFVLNASTGALLWKQTIPNHGDLSSFSTPLLVDGGIVYESSDDGVVQAY
                     NAGGGQLLWRYAI"
BASE COUNT          557 a          812 c          937 g          695 t
ORIGIN      
        1 ggtagccgtg gatggtcagg ccatcgcgcg cggcgaaggt gaaaggctgc atggtggaaa
       61 gggtatagtt cttgagcgcg ggctggttgt cgaagaggta ggtggcgctg cgcgtggcgc
      121 ggtcataggc gtagtacgaa accgggccgt cgtcggtggt gaaggcgata atccaggttt
      181 tgtcggcgtg gtcgcgactg ctcacggcga agtcgccccg gtgcaacgtg cggatgtaat
      241 caaaatcggc gcgaatagat tcatcgagta cgacccattc gaggcgatcc ttcacaaacg
      301 cgacggcctg cacctcgcgt gtatcgggat gcatcatgac actgctcgcg tcgtaatcgg
      361 ggtcttgcgc gatcaccgtc gtgttgccag tggcgatctc cagcttcacc agcctgccag
      421 cgttgacgtt gcgcgagtcc tgcaagtaga gcgcctgccc atccagcgta aagccggcag
      481 gaccgctgga ctggttgtcg tcggcgtccc aggtgagcag cgtactccat tgctcatctt
      541 cgttggcgcg cacgagcaac tccattgcgc cgctgccgtc cggcgcggcg gccatcgcgc
      601 cgcgtacttt cagatgcgca tcgaccaccc atgccaggat attgccgggg ttttgcgtta
      661 ccagtgccag ttcgcccgtc gccaggtcaa gatggtagac atcatgcgat tgcgggttgt
      721 ctttgttcag tcccacgagc aactcgttgg ggaagtgctt gtcgcgcgcc acgactcgcg
      781 cctgcacccg ctcgaatggc gtcaggtcgc gcgtctcgcc gctttgcagg tcagtggcgt
      841 agagccgcca gttctcgtcg cctttgatat cctggatgta caggatatgc cggttatcgg
      901 cggcccaggc atagaagcgc acgccgcgct cggtgtcctg cgtcacaggc cggtaatcat
      961 cctgcccaat cgtcccgacc cacacgttca gcacagtatt gacgggcgcg atataggcca
     1021 tgcgcgtacc atccggcgac acctgcgggc tggacttctc cgggttgccg aacaatatat
     1081 cgcggggaat gagttcgacg gtggatgtgg tggtcatgct tgttccttcg tatcagtctt
     1141 atgtaaagta tctatggcct tcatttcttc catgacttcg tctgcgacgg cctgttcaaa
     1201 ggctacgcga agtgtctcaa ggtcttcggg gcgatgtaat ggcgtcactg aaccaaagcc
     1261 tttgagcaat tgcgattgtt gctgattgtc catattcgac tcctaatact gttgtgagat
     1321 acgggcggct gccttccgat tctaccataa cgtacaaaga attgtagaca tacaagtgta
     1381 ggtgtgttgg tttaccgcgc tcatcgacga ttatgaagtt tctcaacctg aaccggaatt
     1441 gtcaccctga gcgcagcgaa gggtctcgtt cgatgggcag tgagatgctt cgctgcgctc
     1501 agcatgacag caccggctca cgcgattgac tgattacttg cgcccagggt tcgccgggtg
     1561 tgctgaaatg gctcagggaa caaaaccccg gcctctttcg tcaactcctg tagttgatat
     1621 agcacagcac acgttatccg aaagagggct gccatgcgat atgcttctat tctagctcat
     1681 atgaaaagcc gtgtcttcct gtatttactt tttctctgtc tcttcttgat tgtctctgcc
     1741 tgtggggtac acagcggttc cagcaataaa ccgttggggt cgggaatgaa tccagaaccg
     1801 gggacggatg ggaggcctgc gcccgcgccg aatgggcatt acgagtcgat ggttgcgagc
     1861 gatgggattg tttatgtcgg ctccgataat ggcagcctgt atgcgctgaa cggaagcgat
     1921 ggccgcgtgt tgtggcagta tatggcaaat gcgcccgtca gcgttgtggc tgtggttgat
     1981 ggcgcggtct atgcctatgc cgacaaccca aatagtgcgg ttgcgttcgc gttgaatgct
     2041 ggcagtggca atgtagtgtg gcgttaccag gtcagcgatt ttgtctcaaa agcaatggtg
     2101 gtcgataaga tggtctatct cggcactgca gcgacagcga atcagccagt attgtacgcg
     2161 ctgcaaacct ccaggggcgc gctggcctgg cgttatgcag ctcatgccat cacgccgggg
     2221 ctgctcgccg tcagcgatgg agtggtttac tacgcggaga tatcggggat tggcacggcc
     2281 ttcacggaaa acattaccgc gctgggagca ggagatggcc gcgctctctg gcgattgcgc
     2341 acgcagagcg ctgatggcta tgcctatgcc gtcagcgctg tggtgaatgg ggcagtctac
     2401 atcagcacga ataccggggc agtctctgcc gcgcgcgcca gcgacgggac gctgctctgg
     2461 catagtgcgc gcgcttcgat cttcccaggt gtgccgtcgg ctgcaacgcc gctagtcgcg
     2521 gatgggatgg tgtatgtcac cggcaaacag aattttgcca gtcggtcgca gggtctcgat
     2581 gctctgcgcg ccagcgatgg gaagcagctc tggacgcagg cgattgatgg agggcctggc
     2641 ccgcaagtag gtcagccgca actggtggat ggagtgctgt actacagcgg ctcttccggc
     2701 gtaatcgcgc tgcgcgcttc tgacggggcg cgactctggg tatacagcgg cgatacccct
     2761 gccgggccgt tctttgtcag cgatgggcgt gtttatgcca acagcgctgg tggcctgttc
     2821 gttttgaatg ccagcactgg cgcgctgctc tggaaacaga ccatccccaa tcatggcgat
     2881 ctatcaagtt tctcgacgcc cctgctggtg gatggcggca tcgtctacga gtcctcggac
     2941 gatggcgtgg tgcaggcata caatgctggc ggtggtcaac tgctatggcg ttatgctatt
     3001 c
//
LOCUS       DASZAK010000514         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_617489, whole genome shotgun sequence.
ACCESSION   DASZAK010000514 DASZAK010000000
VERSION     DASZAK010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617489"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..700
                     /locus_tag="VGT44_09020"
     CDS             191..700
                     /locus_tag="VGT44_09020"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2580981.1"
                     /translation="MPALETKRLQLIPFSLELKKAALNDRSRLIAMLGVDVPEHWPGP
                     DLAEALPFFIEHMERAPSEPAWDWIAIHRSEQAVIGDIGFMGGPDQQGVVEVGYSIVP
                     EYRNQGYATEMARGLIAWAFQEKGIQVVTAACLDDNFGSIKVLEKIGMRRLEPDGNML
                     KWEIRNEDD"
     gene            complement(831..2111)
                     /locus_tag="VGT44_09025"
     CDS             complement(831..2111)
                     /locus_tag="VGT44_09025"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV2580982.1"
                     /translation="MVSTNVKKNVNNGEITGYCDPRFERVAEEFEQNFRERGEVGASV
                     CVKVAGETLVDLWGGSADPASNTPWVEDTITHVWSCTKGATALCAHMLVSRGLLNLEA
                     PVARYWPEFGQAGKEFIPVKMLLNHQSGVAAISEPIPPGGVYDWGYMVKALERQEPFW
                     KPGSMHGYHGFTFGWLVGEVVRRVSGKSLGTFFREEVAEPLGLDFWMGLPEEHENRVA
                     PIIPADPPDPSGPVSPMMMAIAIDQASMQALLMFNTGGYMLPGPDGVFGFNTRAAHIA
                     EFGAVGSLTNARALAGLYAPLANGGSLNGVSLVSRDTITRMGAVSSASSLDASILLPT
                     RFTLGYAKTVDNRHVPAATAEDSLIMSEEAFGHPGFGGAIGFAEPVANMSFGYAMNRM
                     GPGIGLNARGQSLVDATYLSLGYTSNAGGSWIKG"
BASE COUNT          528 a          660 c          591 g          500 t
ORIGIN      
        1 ttactgctct ctgtttgctg gctcatatag acgctccttg ctcgattcgt ttctgtacaa
       61 gcgatgttat cttgatttag caaattgtaa taccatctga ttatacgccg gatatatcca
      121 aaagttgggc atggattcta tcgaaaacat tcggcatgat gtatgatact ccgcgaagga
      181 agtaatcatc gtgccagcac ttgaaacaaa acgcttacag ctcatcccat tctcgcttga
      241 gctcaaaaag gcagccctca atgacagatc caggctcatc gcaatgcttg gggttgatgt
      301 gcctgaacac tggccaggac ctgaccttgc tgaagctctc ccctttttta tagagcacat
      361 ggaacgagcg ccatctgagc ctgcctggga ctggatcgct atccatagat cggaacaggc
      421 agtgattggc gatatagggt tcatgggagg gccagaccag caaggggttg tcgaagtcgg
      481 gtatagcatc gtccccgaat atcgcaatca aggttatgca accgaaatgg cacgcggcct
      541 catcgcctgg gcctttcaag aaaagggcat ccaggttgtc accgccgctt gtcttgacga
      601 taacttcggc tccataaaag tactcgaaaa gataggcatg cgccgattag agccagacgg
      661 gaatatgctc aaatgggaga tcaggaacga ggacgactaa ccgggcgacc cttgcggtcg
      721 cccggtcctg gggtggtctg cggtggggtg gccgcccggt ggtggggtgg tcgccctgcc
      781 ccggtcgccc ggtcctgggg tggtctgcgg tggggtggtc gcccaatcgc ctacccctta
      841 atccagctcc caccagcatt ggaggtatag ccaagggaaa ggtatgttgc atctaccagg
      901 ctctgacctc gtgcattgag accgatacca ggacccatcc tgttcatagc gtacccaaag
      961 gacatgttgg caaccggctc ggcaaagcct attgctcctc caaagccggg atgtccgaag
     1021 gcctcttctg acataatcag gctatcctct gcggtagccg ctggcacatg ccggttatcc
     1081 acggttttgg catagccgag tgtgaagcgc gtgggaagca atatcgacgc gtccaggctg
     1141 ctcgccgagg aaaccgcacc catgcgtgta attgtgtcac ggcttaccag gctgacgccg
     1201 ttcagactgc cgccattagc gagaggcgcg tacagtcccg ccagggctcg cgcattggtc
     1261 aaactaccga ccgcaccaaa ctcggcgata tgcgcggctc gcgtgttgaa tccaaacaca
     1321 ccatctggac ccggcagcat atatccaccg gtattgaaca tcaaaagcgc ttgcatcgaa
     1381 gcctggtcta tagcgattgc catcatcatg ggagaaacag gcccgcttgg gtcaggagga
     1441 tctgccggaa tgattggagc cacgcggttt tcatgttctt caggcaaacc catccagaaa
     1501 tcgaggccca gcggctccgc cacttcctcg cggaagaagg ttcccagtga cttgccggat
     1561 accctgcgca cgacctctcc aaccagccag ccgaacgtaa atccatgata accatgcatg
     1621 ctgcccggtt tccagaaagg ctcctgcctt tccagtgcct tgaccatata cccccagtcg
     1681 tacacacctc cgggaggaat gggttcgcta atagccgcga ctccagattg gtgattgagc
     1741 aacattttta ccgggatgaa ttctttgccg gcctggccaa actctggcca gtagcgcgct
     1801 accggcgctt cgaggttaag caaacctcgc gaaacaagca tatgggcgca gagggcggtc
     1861 gctcccttgg tacacgacca gacatgggtg atcgtatcct caacccaggg tgtattggat
     1921 gcaggatccg cgctccctcc ccatagatca actaacgtct cgcctgccac tttaacgcag
     1981 accgatgccc cgacctcgcc cctctcacgg aaattctgct caaactcctc tgccacccgc
     2041 tcaaacctcg gatcgcagta tcctgtgatc tcgccattgt tgacattctt cttgacgttg
     2101 gtgctcacca tgaactggtt ctccttttgc ttgctcaaaa taaactggga aagagagagg
     2161 gcggggacaa gccccaccac tactatacac cgtcagatgg cttcttccgc cctgcctaca
     2221 cacgagacga aatgaggttt aacacaacac atcataccac aaatggttga ggtgatacc
//
LOCUS       DASZAK010000515         5401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_368669, whole genome shotgun sequence.
ACCESSION   DASZAK010000515 DASZAK010000000
VERSION     DASZAK010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5401
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368669"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..1407
                     /gene="purB"
                     /locus_tag="VGT44_09030"
     CDS             97..1407
                     /gene="purB"
                     /locus_tag="VGT44_09030"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV2580983.1"
                     /translation="MIERYSRPEMAAIWSAAHKTDTWLKVELLVCEGWAREGVIPPDA
                     MQYIRSASYNAQRMQEIEQETHHDIISFLRSIQEQLGPEGRFIHLGLTSSDVLDTGLA
                     YQIKEAGALLAASLAAAEQVVAQAARRYKYTLMAGRTHGIHAEPMTFGLKLAMWVDEL
                     RRGQQRLAAALEQATVGKISGAVGTHATVPPQIEEYVCEQLGLGIEPISNQIVQRDRH
                     AHFLTTLALLGSSLEKMAQEIRHLQRTELSEVFEPFGSGQQGSSSMPHKRNPELCERI
                     CGLSRVLRGYAVTAMENVALWHERDISHSSAERIIIPDACILLHYMLHIFTHVIGGLE
                     VDEQRMLVNLSMTGGLVYSQRILLALINKGVGRQEAYKIVQRNAKKVWKMASKGAIEG
                     PALLDALSSDAEVARLLSREELIELTNADYYVRYVDTAFAKVGL"
     gene            1404..2948
                     /locus_tag="VGT44_09035"
     CDS             1404..2948
                     /locus_tag="VGT44_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580984.1"
                     /translation="MTLNYSGGEYPGLSPAWLALAFVGISCLYLIAVSRGFHLSRSMS
                     PSQSTSWLLLPLLGALLMGGALLFLPIVFSQDIQSYLDASQAGARYYAYPPAGFRPAS
                     SPALYGPPWLAITSLLVNLSNHNPFLLTLLMRAIELLAHLANTVLIWTILTSIAPLQR
                     LASTLLYAWNPLVLLELVGNAHYDGLVICLLLFSICLYLRRPPSLSAKQPDHVNDTHL
                     ATQSGRDNECSQPESVTNCHPERSEGSHSPGAEILRCAQDDKAGFDSEHSLSRPYDII
                     ALVLVGLAISLNWLALVVAPLLLWFMARSQRGTGSIIKGFSWRVLLVLVVVVITYLPI
                     WQGGTTFVAIVSTIHLQIALNSMLALFVTPLNALYTLLFTRSHITTPSFAPVDPTSAA
                     NLSIVTSALFTFLLLYLREMGKARKVEALFTGLCIVMLAYVALASTIFWPWLLIWIVW
                     LVALRPYDTLSKTLLLFSCTALLYYPLKAAPFALLAPLCIFGIPFVYFIGTRIWTTKT
                     KYEATQ"
     gene            2945..4069
                     /gene="fni"
                     /locus_tag="VGT44_09040"
     CDS             2945..4069
                     /gene="fni"
                     /locus_tag="VGT44_09040"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV2580985.1"
                     /translation="MTDEVKQRKIEHVSVALGQDVSAPQRANWGDIQFVHQALPEVNL
                     DEIDTAVNFLGRTLRYPIFISSLTGGHPDVTTINANLGRAAQRYGLALGVGSQRAAIV
                     NPDVRDSYAVTREQAPDAFLIANIGAPQLIQQERHAAFTLEQVQRAIQMIGADALAVH
                     MNSLQEAAQPEGDRRAGGEAAALKQLTGQITLPVIAKETGAGVCREQALLLRSCGVAA
                     IDVGGAGGSSMSAMEAARSAARGDERTRNIGQLYRDWGIATPIAVVEAGEAQLPLIAT
                     GGVRNGLDMARALALGATLVGMGFPFLKAASQGYDAVCELLETLIAELKVAMQLSGAA
                     TVPALRDVDIVVTGETREWLMMRGFKETLRAMAQRRWRVR"
     gene            complement(4090..4692)
                     /locus_tag="VGT44_09045"
     CDS             complement(4090..4692)
                     /locus_tag="VGT44_09045"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEV2580986.1"
                     /translation="MRFIESPSGIILAGGSSRRMGQDKALLPFPGADPPRTFLEELIR
                     TLDYCCSEVFIVARDQEQASQYAFTDTEIVLDSIPGGGPLVGLASALQAVSTDYAVLV
                     AVDMPFVTPQLLAFMILHYEDGKALVPLVNGHPQVTLAIYPRSILHIIDDLIRQGRRD
                     LRSLLDAAPVRYIDGEELRMVDPALRSFVGVNTPEDLARL"
     gene            complement(4696..5196)
                     /locus_tag="VGT44_09050"
     CDS             complement(4696..5196)
                     /locus_tag="VGT44_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580987.1"
                     /translation="MHFDFAHFNLINWLFTDPCSATNCVDATSTTPATPPETFHFYAV
                     WLIFCSLGLLVAFYYSVEGRKRFVKNNPVAKYMLDRYLGWFAIICILGYPLIFSRAYL
                     YQYFFAWRVWRYAWLVGLLVWGILWILYRVRKYPQERDNYLAYQNRKQYIPRGNRRKA
                     KTASSR"
BASE COUNT         1114 a         1698 c         1534 g         1055 t
ORIGIN      
        1 gtatgcttcc cgtcgccatg taaattcgcg tgtgtgatac actgattagt acagtcacga
       61 gttgcagtgt aacacatcga aggacttttt caaaccatga tcgaacgcta cagccgacca
      121 gagatggcag ccatctggtc agcagcccat aaaaccgaca cctggctgaa agtagaactg
      181 ctggtgtgcg agggttgggc gcgcgaaggt gtcattcccc cggatgccat gcagtatatt
      241 cgctccgcca gctacaatgc gcagcgtatg caggagatcg agcaggaaac ccatcacgac
      301 attatttcat tcctgcgctc tatccaggaa caactcggcc ccgaagggcg ctttattcac
      361 ctgggcctga cctcctccga cgtgctggat accggcctgg cttatcagat aaaagaggcg
      421 ggcgcgctgc tggcggcgtc actggcggca gcggagcagg tcgtagcaca ggcggccagg
      481 cgctacaaat acaccctgat ggccggtcgc acgcatggca tccacgcgga gccaatgacc
      541 ttcgggctga agctggccat gtgggtcgat gaactgcggc gcggacagca gcgcctggcc
      601 gcagcgctgg agcaggccac cgtcggcaaa atctccggcg cggtcggcac ccacgccacc
      661 gtgccgccgc agatcgaaga gtacgtctgc gagcagctcg gcctgggcat cgagcctatc
      721 tcgaaccaga tcgtgcagcg cgaccgtcac gctcatttcc tgacgacgct ggcactgctc
      781 ggcagctcgc tggagaagat ggcgcaggag atccgccatt tgcagcgcac cgaactgagc
      841 gaagtcttcg agccatttgg cagcggccag cagggttcat cgtccatgcc gcataaacgc
      901 aatcccgaac tgtgcgagcg catctgtggc ctgtcgcgtg tactgcgcgg ctacgcggtc
      961 acggccatgg agaacgtagc gttgtggcac gaacgcgata tcagccattc gtccgccgaa
     1021 cgcatcatca tccccgatgc ctgcatcctg ctgcactaca tgctgcacat cttcacgcac
     1081 gtcatcggcg gattggaggt tgacgagcag cgtatgctgg tcaacctgag catgaccggc
     1141 ggcctggtct actcacagcg catcttgctt gcgctcatca acaaaggcgt gggtcgccag
     1201 gaagcctata agatcgtgca gcgtaacgcc aaaaaggttt ggaagatggc cagcaagggc
     1261 gctatcgagg gtcctgccct gctcgacgcg ctcagcagcg atgccgaggt cgcgcggctg
     1321 ctctcgcgcg aagaactgat cgagctgacc aacgccgatt attacgtcag atacgttgac
     1381 accgcgttcg ccaaggtcgg cttgtgacgc taaactactc cggtggcgag taccccggcc
     1441 tctcacccgc ctggctcgcc ctggcattcg tgggcatatc ctgcctgtat ctcattgccg
     1501 tcagccgggg ttttcacctc agccgcagca tgtctccatc gcaatcaaca tcctggctgc
     1561 tgctacctct gctaggcgcg ctgctcatgg gcggcgcgct gctcttcctg cccatcgtct
     1621 tctcgcagga tatccaatcc tatcttgacg cgagtcaagc gggtgcgcgc tactacgcct
     1681 acccacccgc cggtttccgg cccgcctcat cgcccgcact ctacggcccg ccctggctag
     1741 ccatcacctc cctgctggtc aacctcagca accacaaccc attcctgctc accttgctca
     1801 tgcgagccat cgaactgcta gctcacctcg ccaacaccgt cttgatctgg accatactca
     1861 ccagcatcgc cccactgcaa cgccttgcca gcaccctcct ctacgcctgg aaccccctcg
     1921 tcctgctcga actcgtcggc aacgcccact acgacggcct ggtcatctgc ctgctcctat
     1981 tctcaatatg cctctacctc cgccgcccac cgtccttgtc tgcgaaacaa ccggatcacg
     2041 tgaatgacac gcatctcgcg acgcaatcag gacgcgataa tgaatgttca caacccgaat
     2101 ccgtaacaaa ttgtcatcct gagcgcagtg aaggatctca ctcgccgggg gccgagatcc
     2161 ttcgctgcgc tcaggatgac aaggccggtt ttgatagtga acattcatta tcgcgtccct
     2221 acgacattat cgcactcgtg cttgtcgggc tggcgatcag cctgaactgg ttggcgctcg
     2281 tcgtcgcgcc attgttgctg tggtttatgg cgcgcagcca gcgcgggaca ggttcaatta
     2341 tcaaaggctt cagttggcgg gtattgctcg tactcgtagt cgtagtcatc acttacctcc
     2401 ctatctggca agggggcact accttcgtag caatcgtctc aacaatacac ctgcaaatcg
     2461 ctctgaattc catgctggca ttgttcgtga cgccgctgaa cgcgctctac actctgctgt
     2521 ttacccggag ccacatcacc acaccctcct tcgcgccggt cgatcccact tccgccgcga
     2581 acctgtcgat tgtcacctca gcgcttttca cctttctcct gctctacctc cgggaaatgg
     2641 gaaaggcgcg caaggtggaa gcgctgttta ccggcctgtg cattgtaatg ctcgcatatg
     2701 tagcgcttgc ctccacgatc ttctggccgt ggttgctcat ctggatagtc tggctggtag
     2761 cgctgcgccc ctacgatacc ctctcgaaaa ccctgctgct cttctcatgc accgcgctcc
     2821 tctactatcc cttgaaggca gcgcctttcg ccctcctggc acccttatgc atctttggca
     2881 taccgttcgt ctatttcata ggaacgcgaa tttggactac aaaaacgaaa tatgaggcta
     2941 cccaatgacc gatgaagtca aacaacgcaa gattgaacac gtcagcgtcg ccctggggca
     3001 ggatgtctcc gcgccgcagc gcgccaactg gggcgacatc cagttcgttc accaggcgct
     3061 gccggaggtc aacctggatg agatcgatac cgccgtcaac tttctcggtc gcaccctacg
     3121 ctatcccatc ttcatctcct cgctgacggg cggccacccc gatgtcacaa ccatcaatgc
     3181 gaacctgggg cgcgcggctc agcgctatgg actggccctc ggtgtaggca gccagcgcgc
     3241 cgccatcgtc aaccccgacg tgcgcgacag ttatgccgtg acgcgcgaac aggccccgga
     3301 cgcctttctg atcgccaata tcggcgcgcc gcagttgatc cagcaggagc gccacgcggc
     3361 tttcaccctc gagcaggtgc agcgcgccat ccagatgatc ggggctgacg cgctggccgt
     3421 gcatatgaac agcctgcaag aggccgcgca gccggagggc gaccggcgcg ccggaggcga
     3481 ggcggcggcg ctgaaacagt tgaccggaca aataaccctg cccgtcatcg caaaggagac
     3541 gggcgcaggc gtctgccgcg aacaggcgct gctcttgcgc tcgtgcggcg tggcggccat
     3601 cgatgtcggc ggcgcgggcg gcagcagcat gtccgcgatg gaggcggcgc gttccgccgc
     3661 gcgcggcgac gaacgcacgc gcaacatcgg ccagctttac cgcgattggg gcatcgccac
     3721 gcccatcgcc gtggtcgagg caggcgaggc gcagctcccg ctcatcgcca ccggcggtgt
     3781 gcgcaacgga ctggacatgg cgcgcgcgct ggcgttgggc gcgacgctgg tcggcatggg
     3841 cttccccttc ctcaaagccg ccagccaggg ctacgacgcc gtctgcgaac tactggaaac
     3901 gctcatcgcc gaactgaaag tagccatgca gctcagcggc gcggcaaccg tccccgccct
     3961 tcgcgatgtc gatatcgtcg tcaccggcga aacgcgcgag tggctcatga tgcgcggctt
     4021 caaggagacg ctgcgcgcga tggcgcagcg gcgctggcgg gtacgctaga aaactatcac
     4081 attgaaaccc taaaggcgag cgaggtcctc cggcgtattc acgccaacaa acgagcgcaa
     4141 cgccgggtca accatgcgca actcctcgcc atcgatataa cggacgggag cggcatcgag
     4201 caggctgcgc aggtcgcggc ggccctgccg gatcaggtcg tcgatgatgt ggagaatcga
     4261 gcgcgggtag atcgccagcg tgacctgggg atggccgttg acgagcggta ccagcgcctt
     4321 tccatcctcg taatgcaaaa tcataaaggc gagcaattgg ggtgtgacga agggcatatc
     4381 tacagcgacg agtactgcat aatctgttga gacagcttgc agcgcgctgg cgaggccgac
     4441 cagcgggcct cctccgggga tgctgtcgag gacaatttcc gtgtcagtaa aggcatactg
     4501 tgacgcttgc tcctggtcgc gggcaacaat gaatacctct gaacagcaat aatctagcgt
     4561 gcgtatgagt tcctcaagaa acgtcctggg cggatcagcg ccgggaaaag gcagcagcgc
     4621 tttgtcctgg cccatgcgtc ggctgctgcc ccctgccaaa atgataccag agggactctc
     4681 tatgaacctc atagtctagc ggctggaagc agttttcgct ttgcgcctgt tgcccctggg
     4741 aatgtactgc ttgcggttct ggtacgccag gtagttatcg cgttcctgcg ggtatttgcg
     4801 cacgcggtac aagatccaga gaatgcccca gaccagcagg ccaaccagcc aggcgtaacg
     4861 ccagacacgc cacgcgaaga agtattggta caggtaggcg cgcgagaaga tcaggggata
     4921 ccccaggatg cagataatcg cgaaccagcc aagatagcga tccagcatgt acttcgcaac
     4981 cgggttattt ttcacgaagc gcttgcggcc ttcgaccgaa tagtaaaatg cgaccagcag
     5041 gcccaggctg caaaaaatga gccagaccgc gtagaaatgg aacgtctccg gcggcgtggc
     5101 cggggtggtt gaggttgcat ccacacagtt tgtggccgaa cacgggtccg taaaaagcca
     5161 gttaattaag ttgaagtgcg caaaatcgaa atgcatagcg ttccgcccct tctgcttatc
     5221 aatcaagttc cggtatcgtt gcatatgata acatggttga agtgaagcgt ctagagggcg
     5281 aattgactca ccaaaaatct tactttggcc gaaacgttgc ctcatgagag aatcttacca
     5341 gccgctgttg tggaagagcc aggcggctgg aaggaagaga ggccatgatg agtgtcacga
     5401 g
//
LOCUS       DASZAK010000516         2740 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_617547, whole genome shotgun sequence.
ACCESSION   DASZAK010000516 DASZAK010000000
VERSION     DASZAK010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617547"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            407..2011
                     /locus_tag="VGT44_09055"
     CDS             407..2011
                     /locus_tag="VGT44_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV2580988.1"
                     /translation="MAETNISQEQQDEALLQKFGYKQELRRTLGFISNFAVAFSYISV
                     STGTFSLFALGIVAGGPAFFWTWPIVAIGQFIVALNFAELASHFPIAGSIYQWSKRLS
                     GYSLGWFTGWFYFFAGVLTITGVAITIPIPLIAIFPGIPPTILGMPNNVFIALVAIIV
                     GTLINVAGVRLVSFINNIGVAAEIIGMIGFALVLLIFFNHQSLGFLFTQSTVTSQSLG
                     ADWTPGFGSTYFGAFAAAMFMSLFVIYGFDTAGTLGEETKDPQKNAPRGVLWAIGLSA
                     IAGVLFLGGTILSIKDPGFIENIAAGGNKNIAPTGALPTIIQQALGTVWGNIYLTVVL
                     VAVCVCTLAIQSATIRLMFSMGRDGRLPVGKVWGTVNPTFRTPLYAGIGVAILSAIPL
                     LALINLPNSIGIIVVAATGLIYFSYFLNNIASLIARFHGWPRVKSPFSLGSWGVLVNV
                     LALIYGGVMLVNFLWFGGLRDTFATNPSLNTVFGGNIGSNFFGTFPLFELSLIVLFVV
                     GSIYWFGYKRRQVIASGERRAEALAD"
     gene            2218..>2740
                     /locus_tag="VGT44_09060"
     CDS             2218..>2740
                     /locus_tag="VGT44_09060"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580989.1"
                     /translation="MNVLTMPGKRLFLTGDEAVARAALEAGVEVATGYPGNPSTKTIT
                     ALIPIAAQYGMTVEWSVNEKVALEVATGAAWAGKRSLVAMKMSGINVMADSLLSIAHS
                     GVLGALVIYAVDDVGVYYGMVEQDSRYYARLTSLPMLMPSSPEEAYHMTRLAFAMSEH
                     TGAPVFVLGTTAVA"
BASE COUNT          552 a          767 c          744 g          677 t
ORIGIN      
        1 catctcacat gttggctcta gatgcttcgc tgcgctcagc atgacagtgc tcattgtatg
       61 ccttgaaatg tgcctatagc aatgtgcacg cgagatgctt cgctgcgctc agcatgacaa
      121 tgctcattgt atgacctggc attggcatct ggcaacgcac tccctcacag acccgtctcg
      181 atttctgtaa aacatccaca ttttgtagga agaatgtgga tagattgttt attggcaagt
      241 ggttatggta aactctacta tgtaccataa cagtagcaaa attgggctta ctgtttcctc
      301 ttttcatttc acgcttccgg cagctcatca aaccggacgt gtgaaggctt ttttcgtcaa
      361 gcgtgtctta tcgttggtgt tatcaccaca aaggaggcgt gtccacatgg cggaaaccaa
      421 tatctcccag gagcaacagg acgaggcatt actgcaaaag tttgggtaca agcaggaact
      481 gcggcgtacg ctcggtttca tctccaactt cgcggtagca ttctcctata tctccgtctc
      541 gaccggaacg ttttcgcttt tcgcactcgg catcgtggct ggcggcccgg cgttcttctg
      601 gacgtggccg atcgtcgcca ttgggcagtt catcgtcgcg ctcaacttcg ccgagctggc
      661 ttcccatttc ccaattgcag gctcgattta tcaatggagc aagcggctct ccggctacag
      721 cctgggctgg tttacgggct ggttctactt tttcgccggg gtgctgacca tcaccggcgt
      781 cgcgatcacc atacctatcc ccttgatcgc cattttccca ggtattccgc cgacgattct
      841 gggaatgccg aataacgtct ttatcgcgct ggtggcgatt atcgttggca ccctcatcaa
      901 cgtggccggt gtgcggctgg tgtccttcat taacaatata ggcgtcgcag ccgagatcat
      961 cggcatgatc ggcttcgcgc tggtgctgct gatcttcttc aaccatcaat cacttggctt
     1021 cctgttcacg caatccaccg taacgagcca gtcgctcggc gcggattgga cgcccggctt
     1081 cggcagtacc tactttgggg cattcgctgc agctatgttc atgtcgctgt tcgtaattta
     1141 cggcttcgac acagctggca cacttggtga ggaaacgaag gacccgcaga agaacgcgcc
     1201 gcgcggcgtt ctctgggcaa ttgggctgtc cgctatcgcc ggcgtcctct tcctgggcgg
     1261 gacaattcta tcgataaaag atcccggttt tatcgagaat atcgcggcag ggggaaataa
     1321 aaacattgct ccaaccggcg cgcttccgac catcattcag caagcgctgg gaaccgtctg
     1381 gggcaacata tacctgacgg tcgtactcgt cgcggtttgc gtttgcaccc tggcgatcca
     1441 gtcggctacc attcgcctga tgttctcaat ggggcgcgat ggccgtctgc cagttggcaa
     1501 agtctgggga acggttaatc cgaccttccg cacgccgctc tacgcaggta ttggagtagc
     1561 cattctctct gccatccctc tgctagcgct gattaacctg ccgaattcga ttggtatcat
     1621 cgtggtcgcg gcaacagggc taatctactt ctcgtacttc ctgaacaaca tcgcctcgct
     1681 gatcgctcgg tttcacggct ggccacgtgt gaaaagcccc ttcagtctgg ggagctgggg
     1741 cgtactggtg aacgtgctgg cgctcatcta cggcggcgtg atgctcgtca atttcctctg
     1801 gttcggtggt ctgcgcgata cctttgctac caaccctagc ctgaatacag tcttcggagg
     1861 aaatattggg agcaacttct tcggcacctt cccgttgttt gaactctcgc tgattgtgct
     1921 cttcgtcgtc ggcagcatct actggttcgg ctacaagcgc cgccaggtaa tcgccagcgg
     1981 cgaaagacgc gccgaggcgc tggcggactg aagcagttgt aacaaagtga taacaaaaca
     2041 gcactagcaa cccgccgaac atggcgaccc tcgcggtcgc catgttcgca gttttgttat
     2101 cactttgtta cataccttcc ttgacaaacc gcaaacctat gccatacact agctacatta
     2161 gagaaaatct atctgctgtt cccattggag tgtcgtcgcg ggaaaaggaa cccagtcgtg
     2221 aatgttctga ccatgccggg caaacggctt tttttgacag gcgatgaagc tgtagcgcgt
     2281 gccgcattag aagcgggagt ggaggtcgcc accgggtatc cgggcaaccc cagcacgaag
     2341 acaattaccg cgttgatccc cattgctgca cagtatggca tgaccgtcga gtggagcgtc
     2401 aacgagaaag tcgcgcttga agtggcgacc ggcgcggcct gggcgggcaa acgcagcctg
     2461 gtggcgatga agatgtcggg catcaacgta atggcggatt cgctgctctc tattgcccac
     2521 agtggtgtgc tgggcgcgct ggtgatttat gccgtcgatg atgttggcgt gtactacggc
     2581 atggttgagc aggatagccg ttactatgcc cggctgacct cgctgccaat gctgatgcca
     2641 tccagcccgg aagaagccta ccacatgacg cggctggctt tcgccatgtc ggaacacacg
     2701 ggcgcgcccg tctttgtgct gggaacaacg gcggtagcca
//
LOCUS       DASZAK010000517         7825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_581306, whole genome shotgun sequence.
ACCESSION   DASZAK010000517 DASZAK010000000
VERSION     DASZAK010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7825
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581306"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..613)
                     /gene="efp"
                     /locus_tag="VGT44_09065"
     CDS             complement(41..613)
                     /gene="efp"
                     /locus_tag="VGT44_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV2580990.1"
                     /translation="MGATTTADFYNGMVIRYNGELYTVTEFHHVSPGNWRAFVRVNLK
                     NLRTGRMIEQRFRAGEEVDAVRVEHQNWQFLYVDGDDYIFMNTETFDQQPIPKAMLGD
                     SVRFLKEQDIVELLVDSETDTIVAVEMPNFLELTVESAEPGVRGDTANNVTKPATLET
                     GTVINVPLFVNPGDKIRIDTRTGQYVSRVK"
     gene            complement(721..1170)
                     /locus_tag="VGT44_09070"
     CDS             complement(721..1170)
                     /locus_tag="VGT44_09070"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEV2580991.1"
                     /translation="MMTSTSEIRRVFIDTSAWISLMNKNEFNHEQAVAFHKSLPTTIY
                     RITTWGIVSETFTWTRYHIGSRAGLQWLSLKEALERQGLLQIVFPDPQMEIGVKKIIE
                     RFHDQKLSYVDAYSIALIKSRPDIDVIFAFDRHMALAGIPVLPGTLK"
     gene            complement(1151..1372)
                     /locus_tag="VGT44_09075"
     CDS             complement(1151..1372)
                     /locus_tag="VGT44_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEV2580992.1"
                     /translation="MELVRKQTYITPAQDKAVKQLAHQQGITEAEILRKALDQFLAKA
                     GIVEGEDPFAVLIGMFEGPLEVDHDDIYL"
     gene            complement(1431..2225)
                     /locus_tag="VGT44_09080"
     CDS             complement(1431..2225)
                     /locus_tag="VGT44_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580993.1"
                     /translation="MHDTLLPLIERALAENTRPLEFYLREQSHLPGTRANLGLVGEVS
                     DELASMVARHSQQVWELLRTLSQDALVVESNTPAEFVALCGAVAFGACAAAHPDWQPD
                     VRVLLSRMSSSASWRVREGVAMAYQRLLTVAPEATLVYLRDLAADGDCLQQRACVAAV
                     AEPILLQTEQMKEGALGIQGVIVHRFHDIPLSERKREDVRILRQGLSFTLSVAVAALP
                     EAGFTLLRECASWGDPDINWIIRENLKKKRLLKFTEYSAHLASMLA"
     gene            2415..2909
                     /locus_tag="VGT44_09085"
     CDS             2415..2909
                     /locus_tag="VGT44_09085"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2580994.1"
                     /translation="MLATNEQEFLSGSDAACAYSIEQVAAQTGLTKRTLRYYEEVGLL
                     PPTDRTEGNYRRYSDEDIRRLERIKMLRDLLGFALADIREILETEDERGVLREAYKHA
                     SEAAAKISQLDQSDELIRKQLKLIEQKIEGLGQMRASLLQQIERHALKRRTLQESDTR
                     QSME"
     gene            2943..4262
                     /locus_tag="VGT44_09090"
     CDS             2943..4262
                     /locus_tag="VGT44_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2580995.1"
                     /translation="MQTLTSDDREQNVPGPIIEHEVNGLLRTFIALRHRNFRLFWFGQ
                     LISLVGTWMQTTAQAWLVLVLTHSAWLLGLVGALQFLPVMVFSLFGGVLADRLPKRTA
                     LLFTQSSALIQATVMWILVATGTVQIWHIMLLAALLGLTNSLDMPTRQSFVVEMVGRE
                     DLPNAVALNSSIFNTARVLGPGLGGLLIAWLGVAPLFLLNAISFVAVLIGLAMINQKQ
                     LFALPTSGGKRSSTLKSLREGLAYVRHMPSVLLVINVIGVISLFGINFNVMLPLFATQ
                     VLHSGPQGFGFISSAFGLGSLFSALWIAWGNHRPSIRYMLVAALAFCLLEIVFALSHI
                     FVLSLVLIASVGFAQIAFSATANTTLQTVTPDRLRGRVMSVYMMVFAGSVPPGNLFAG
                     GLALLWGAPVSLLIGAGLSLVAAVVAWIMRKPAEQSLAESSPAHVVE"
     gene            4279..5055
                     /locus_tag="VGT44_09095"
     CDS             4279..5055
                     /locus_tag="VGT44_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580996.1"
                     /translation="MVIANQQQHIDQVERLLSAIQDEPSSQAPEARFFWSQADSEGLV
                     LGFSQKPDVLNPVEMERRPLPVYHRRAGGTAVLVGPHLLGLDVLLPAGHPLILPDLVE
                     SYRWLGETWVAALRLLGVETRAVPPDEAHTQRGLLKQPASREREAILRRACYGSLSPY
                     EVVAGQRKVVGLDMIRRRAGTLLQAGLLLRWDTERLALLLGHSAAEQAILREGLRERA
                     VGLDELAGRVVPAQEAIEAFEQAIAERKDLLDIIFSSDLY"
     gene            5097..5897
                     /locus_tag="VGT44_09100"
     CDS             5097..5897
                     /locus_tag="VGT44_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEV2580997.1"
                     /translation="MADRNAPGALQVIQTPDYEAMVIGAHPDDDDFGAAGTSALWAKQ
                     GKKIVWVVMTDGAEGSEVPSLVDTDLMLAREQEQRMACEVLGVQDVEFLRFPDGHLTN
                     SEAARKAIVRLIRKYRPRVVFTHDPTTHVFAPDPDEKPEETAYLNHPDHRATGNIVLD
                     AIFPAVGNPRSYRELLAEGFPPYQVHELYLFFSDHENTYIDISETIEVKAKGLQCHVS
                     QFGQEANMLEMLKKWGEGTAKEAKEKKGLEMQYAESFRRVKLHIPKVE"
     gene            complement(5906..6862)
                     /locus_tag="VGT44_09105"
     CDS             complement(5906..6862)
                     /locus_tag="VGT44_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2580998.1"
                     /translation="MLLILVGLLAFALLVTMTGYLLSNKPREDGASYPYARASRHDRD
                     TEPVRNPYARRTNVMPAQRYKRSATTQTLPRGARVMSVPTWGAGLSLGRGGTGERVPW
                     SIVVIGLVSVCILGIFAFNAIFPHSAILIPTWFANSSAQPKSTQPASQPVSFFGASKA
                     LQRIGQLDPAQYNSVQDYNVWAYSACSTAAMTEVFNAYGKHFRIADVLKVEAGINEIT
                     PSLGLVSDVGIQRTAALFGFNTSWGYSLSLSKIIGIANSGEPVIAGWPPSKYPGGHLV
                     VVIGGNATTVFLADSSRYDRTSLTYAQFMAWWGGFSAVVTPI"
     gene            7119..>7825
                     /locus_tag="VGT44_09110"
     CDS             7119..>7825
                     /locus_tag="VGT44_09110"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV2580999.1"
                     /translation="MMSPELQTLPIRAIDCLKLCYKLHEQDEAITTSVMRERLQPLEP
                     TGQLSDATVTQLFKWLGERGYVRYIPYHGVELTETGAATAAELVRRHRLLELFLVQIM
                     GFSLDEVDTEAEQLEHAISDTFEERMDTLLGHPTEDPHGDPIPDRQGVVVVSSSQPLA
                     TVLPGQHVIVQRVSDENPDLLRYLTSLGLIPGALVCVEDVAPYGGVYTLRIGEQTHAV
                     GEVVTQRVLVRPANMART"
BASE COUNT         1689 a         2119 c         2233 g         1784 t
ORIGIN      
        1 gttcgcggag cgaacagtgg ctgctatttg tgtatcaata ttattttacg cgcgagacgt
       61 actggcccgt gcgggtatcg atgcgaattt tatcgccagg attgacgaag aggggaacgt
      121 tgatgaccgt gccggtttcc agcgtagcgg gcttggtgac gttgttggcg gtatcgccgc
      181 gcacaccggg ttcggcgctt tcgacggtca attcgaggaa gttcggcatt tctacggcga
      241 cgatggtgtc ggtttcgcta tccaccagca gttcaacgat atcctgctct ttgaggaagc
      301 gcacggagtc gcccagcatt gctttcggta taggctgctg atcgaacgtt tcggtgttca
      361 tgaagatata atcatcacca tcgacataca aaaattgcca gttctggtgt tcgacgcgca
      421 cggcgtccac ttcttcgcca gcgcggaagc gctgctcgat catgcgaccc gtgcgcaaat
      481 tcttgaggtt tacgcgtaca aatgcgcgcc agttcccagg actgacatgg tgaaactctg
      541 tgactgtgta caactctccg ttgtacctga taaccatgcc gttataaaaa tctgctgtag
      601 ttgttgcgcc caatggagtg aactccttcc tttccttcat agcaccatag catcatacgt
      661 ttttagcgtc tcctattgta gtggaatgta gcctattctg tcaaagttgg tttagagagc
      721 ttacttgaga gttcccggca atactggaat acctgccagg gccatgtgac gatcaaaagc
      781 gaaaattaca tcaatatctg gacgtgattt gataagtgct attgagtaag catcgacata
      841 agacaacttt tgatcgtgga aacgctcaat gattttcttc actccgattt ccatttgtgg
      901 gtcaggaaag acgatctgta gtaatccttg acgctcgaga gcctctttca gtgaaagcca
      961 ctgaagccct gctctgcttc cgatgtggta acgagtccag gtaaaagttt ctgatacgat
     1021 gccccacgta gtaatacggt aaatcgttgt gggaagactt ttatgaaaag ctaccgcctg
     1081 ttcgtgattg aactcgtttt tattcatgag gctgatccag gcggaagtat ctataaatac
     1141 tcgcctgatt tcagaggtag atgtcatcat gatccacctc taacggccct tcgaacatcc
     1201 cgatcaatac agcaaatggg tcttcgcctt caacaattcc tgcctttgca agaaactggt
     1261 caagagcttt gcgaagtatt tctgcctctg taataccttg ttgatgtgca agttgcttta
     1321 cagctttatc ctgagcagga gtgatataag tttgttttct aacgagttcc atagtagctt
     1381 gcctccttga tgtcattcta caacatcata gagattataa caaggggaat tcatgccagc
     1441 atacttgcca ggtgggcgct atactcggta aacttcaaga ggcgtttctt tttcaaattc
     1501 tcgcgaatga tccagttgat gtcggggtcg ccccaggagg cgcattcgcg cagcagggta
     1561 aagcctgctt cgggcagcgc ggcgacggcg acgctgaggg taaagctcag gccctggcgc
     1621 aggatgcgta catcttcgcg cttgcgttcc gagaggggaa tgtcgtggaa acgatgcacg
     1681 atcacccctt gaatgcccaa cgcgccttcc ttcatttgct cggtctgcag aagaatgggt
     1741 tccgcgaccg ccgcgacgca ggcacgttgt tgcaagcaat cgccatcggc agccaggtcg
     1801 cgcaggtaga ccagcgtcgc ctccggcgct acggtgagca ggcgttgata ggccatcgct
     1861 acgccctcgc gcacgcgcca cgacgcgctg ctcgacatac gcgagagcag cacacgcacg
     1921 tcgggttgcc aatcaggatg ggcagcagca cacgcgccaa aggcaactgc tccgcagagc
     1981 gccacaaatt cggcgggcgt gttgctctcc acgaccagcg catcctggct cagcgtgcgc
     2041 aacagctccc atacctgctg cgaatgcctg gcgaccatcg aggccagttc atcgctgact
     2101 tcccctacca atcccaggtt cgcgcgcgta ccgggcaggt ggctttgttc gcgtaagtaa
     2161 aactctaacg gtcgtgtgtt ctccgccaat gctcgttcga tcagtgggag gagcgtatca
     2221 tgcattatcc tacttccttc atgcttctgt gagctatgtt ttcaagcgtc gtatgtttac
     2281 ccttcctatt ttatcatact agcgcttttg ttaccgcaaa tcataacatt gacgttaacg
     2341 gcaacgttat ggtataatgg tatcatccaa ccggatgagt atgatactga taaactctgg
     2401 ggaagagcat accaatgctt gcaacgaatg aacaggaatt tctttccggc agtgatgctg
     2461 cctgcgccta ttccattgaa caggttgccg cgcaaactgg cctgaccaag cgtacactgc
     2521 gctattacga agaggttggc ctgttgccgc cgacggatcg cacagagggc aattaccggc
     2581 gctacagcga cgaggatatc aggcggctag aacgtattaa aatgctgcgc gatctgctgg
     2641 gttttgctct ggcggatatt cgcgagattt tagaaactga agatgagcgc ggtgtgctgc
     2701 gcgaagcgta caaacatgca agcgaggcgg cggcgaaaat ctcgcaactc gatcaatctg
     2761 atgagttgat ccgcaagcag ctcaagttaa tcgagcagaa aattgaggga ctggggcaaa
     2821 tgcgagcatc actcctgcaa cagatcgaac gccatgcgct caaacgcagg acattgcagg
     2881 aatcagatac ccggcagtca atggaataat ctaacgcttt ttgaaacact ggatcggaca
     2941 ctatgcaaac acttacttct gatgacaggg aacagaacgt tcccggacct attattgaac
     3001 acgaggtcaa tggactgctg cgaacattca tagctctgcg gcatcgtaat tttcggctct
     3061 tctggtttgg ccaacttatc tcgctggttg gcacctggat gcaaaccacc gcgcaagcat
     3121 ggctggtgct ggtattgacg cacagcgcgt ggctgctggg gctggtaggg gcgctgcaat
     3181 tcttgccggt gatggtcttc tcgctgttcg gcggtgtgct ggccgaccgg ctgcccaaac
     3241 gcacggccct gctttttacg caatcgtcgg ctttgatcca ggccaccgtc atgtggatac
     3301 tggtcgcgac aggaaccgtg cagatctggc atatcatgct gctggctgcg ctgctgggac
     3361 tgactaactc gcttgacatg cccacgcgcc aatcatttgt cgttgaaatg gtcggacgcg
     3421 aagacttgcc caacgccgtc gcgctcaatt cttcgatctt caacacggcg cgcgtgctgg
     3481 gaccgggact gggcggcctg ttgatcgcct ggctgggcgt cgcgccgctc ttcttgttga
     3541 atgcgatcag ttttgtcgcc gtccttatcg gattggcgat gattaatcag aagcaactct
     3601 tcgcgcttcc aacgagtggc ggcaaaaggt caagcactct caagagcctg cgcgagggtc
     3661 tggcgtatgt ccgccatatg ccttccgtct tgctggtgat caacgtgatc ggtgtgatct
     3721 cgctcttcgg catcaacttc aacgtgatgc tgcccctgtt tgccactcag gtgctgcatt
     3781 ctgggccgca gggttttggc ttcatctcgt cggcttttgg gctggggtcg cttttctcgg
     3841 cgttgtggat cgcctggggc aaccacagac cgagcatccg ctatatgctg gtcgcggcgt
     3901 tggcattctg cttgcttgaa atcgtcttcg cgctctctca catctttgtg ctttcgctgg
     3961 tcttgatcgc cagcgtgggg tttgcgcaga tcgcgttctc ggcaaccgcc aacacgacgc
     4021 tgcaaaccgt cacgcccgat cgcctgcgcg gtagggtcat gagtgtctac atgatggtct
     4081 tcgccggcag tgtgccgccg ggcaacctct ttgcgggcgg cctggcgctg ctgtggggcg
     4141 cgcccgtctc actgctgatt ggggccggcc tcagcctggt tgcggcagtg gtggcatgga
     4201 ttatgcgcaa accagcggag caaagccttg cagaatcttc ccccgcccat gtcgtcgaat
     4261 agccggagca tagcgccaat ggtcattgct aaccagcagc agcatatcga ccaggtcgaa
     4321 cgcctgctct cagctattca ggatgagcct tcgtcgcaag cgcctgaagc gaggttcttc
     4381 tggtcacagg cggatagtga aggtctcgtg ctgggcttct cgcaaaagcc agatgtgctg
     4441 aacccggtgg agatggagcg caggccgctg ccggtctacc accggcgcgc gggcggcact
     4501 gccgtgctgg tcggcccaca tctgcttggc ctggatgtgc tgctgcccgc cggtcatcca
     4561 ctgatcctgc ctgacctcgt ggagagctat cgctggcttg gcgagacctg ggtagcggcg
     4621 ctgcgactgc taggcgtcga aacacgcgcg gtcccgcccg acgaagccca cacccaacgc
     4681 ggtctcctga aacagccagc atcgcgcgaa cgtgaggcga tcctgcgccg cgcctgctac
     4741 ggctcgctct ccccctacga agtggtcgca ggccagcgca aggtcgtcgg gctggacatg
     4801 atccgccgtc gcgcgggcac gctgctccag gccggtctac tgctgcgctg ggataccgaa
     4861 cggctcgccc tgctgctcgg ccacagtgcc gcggagcagg ccatcctgcg cgaaggactg
     4921 cgcgagcgag cggtcgggct tgacgaactg gcaggcaggg tggtaccggc gcaggaggca
     4981 attgaagcgt ttgagcaggc catagctgag agaaaggatt tgcttgacat catcttttcc
     5041 tcagacctat actgaacaca tcgtacaatt ggcatactca ggaagggcta aactacatgg
     5101 cagatagaaa cgctccgggg gcgttgcagg taatacagac accggattac gaggcgatgg
     5161 tgatcggggc gcatcccgat gacgatgatt ttggcgcggc tggcacgagc gcgctgtggg
     5221 cgaagcaggg caagaagatc gtctgggtcg tcatgaccga tggcgctgag ggcagcgagg
     5281 ttcccagcct ggtcgatacc gatttgatgc tggcgcgcga gcaggagcag cgcatggcct
     5341 gcgaggtgct gggtgtacag gatgtcgagt tcctgcgctt cccggacggg catctcacca
     5401 actccgaagc ggcgcgcaag gccatcgtgc gcctgattcg caagtatcgc ccgcgcgtcg
     5461 tcttcacgca tgatccgacg acccacgtgt ttgcgcccga ccccgacgaa aagccggagg
     5521 agaccgctta cctcaatcat cccgaccatc gcgccaccgg caatatcgtg ctggacgcca
     5581 tcttccccgc cgtaggcaac ccgcgttcgt accgcgaact gctggccgag ggatttccgc
     5641 cctaccaggt tcacgaactc tacctattct tttccgatca cgagaacacc tacatcgaca
     5701 tcagcgaaac catcgaggtc aaagcgaagg gtctacaatg ccacgtctca cagtttggcc
     5761 aggaggccaa tatgctcgaa atgctgaaaa agtggggcga aggcacggcc aaagaggcga
     5821 aagagaagaa gggactggag atgcagtacg ccgaaagctt ccgccgggtg aaactgcata
     5881 taccaaaagt agaataatct ctcgatcaga tgggcgttac cacagccgaa aatccgcccc
     5941 accaggccat gaattgcgcg taggtcagcg aggtgcggtc gtagcgcgac gagtcggcga
     6001 ggaagacggt ggtggcattg ccgccaatca cgacgaccag gtggccgccg ggatatttgc
     6061 tgggcggcca ccccgcgatg accggctcgc cgctattggc tattcctatg atcttgctga
     6121 gcgagaggct gtagccccag gaggtattga agccgaagag ggcagccgtg cgctggatgc
     6181 ctacatcaga aaccagtccc aggctgggcg tgatctcgtt gatccccgcc tcaaccttaa
     6241 gaacgtccgc gatacgaaaa tgtttgccat aggcattgaa gacttcggtc atagcggcgg
     6301 ttgagcaggc agaatacgcc cagacattgt agtcctggac agaattgtac tgcgcgggat
     6361 cgagctggcc aatgcgctgc aaggcttttg atgcgccaaa gaaactcact ggctggctgg
     6421 ctggttgagt tgatttcggc tgcgcgctgc tgttggcaaa ccaggttggg atgaggatgg
     6481 cgctgtgcgg gaagatggca ttgaaggcaa atatccccag aatgcagacc gaaacgagtc
     6541 caatcaccac aatcgaccag ggtacgcgct cacccgtacc gcccctcccc aggcttaaac
     6601 cggctcccca tgttggaact gacataactc gcgcaccacg cggcagcgtt tgggtcgtcg
     6661 ctgaacgctt gtaacgttgc gcgggcatga cgtttgtccg cctggcatat gggttcctga
     6721 ccggctcggt atcgcggtcg tgcctggaag cgcgcgcata aggatacgac gcgccgtctt
     6781 cgcgaggctt gttcgacaat aaataaccgg tcatcgtgac aagcagcgcg aaggcgagca
     6841 gtccaaccag tatcaagagc atgaattgcg gctcctggca ggctatgtgg caaatagcga
     6901 tgagtgacga aatgaattag gacaaatttt caaacttctg tccttactgg taactatacc
     6961 acaagcattt gtaatattca acggtaattg aagaggaatt ttcgtctcta cggttgcctt
     7021 tttcttttaa gagaccttga caatggttta ctctatgtgg tattaatatt ttagagtata
     7081 aaaatttcct attctgtggg ataaactatg aggttttgat gatgtcacca gaattacaga
     7141 cgctgccgat tcgcgctatc gattgtctaa aactttgcta taagctgcac gaacaggatg
     7201 aagccataac gacatccgtg atgcgagagc gcttgcagcc gttggagccg acgggacaat
     7261 tgagtgatgc cactgtcaca cagcttttta agtggctggg tgagcgcggg tatgtacgtt
     7321 atattccgta tcatggcgtt gagctgacag aaacgggcgc ggccaccgca gcagaacttg
     7381 tgcgccgcca ccgcctgctc gaactcttcc tggtacagat catgggattc tcattggacg
     7441 aggtggacac ggaggcagag caacttgaac atgctatctc tgataccttt gaagagcgca
     7501 tggatacgct gctggggcat ccaacggaag acccgcatgg cgaccccatc cctgacaggc
     7561 agggggtagt ggtggtttct tccagtcagc cgctcgcgac ggtgctgccg gggcagcatg
     7621 tcatcgtgca gcgcgtgagc gacgagaatc ccgatctgct gcgctacctt acctcacttg
     7681 gtttgatacc cggcgcgctg gtctgcgttg aggatgttgc gccgtatggc ggcgtgtaca
     7741 ccctgcgcat cggggaacaa acgcacgcgg taggagaggt ggtcacacag cgtgttcttg
     7801 ttcgtcccgc caatatggcc aggac
//
LOCUS       DASZAK010000518         3127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_84408, whole genome shotgun sequence.
ACCESSION   DASZAK010000518 DASZAK010000000
VERSION     DASZAK010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3127
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84408"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1019
                     /locus_tag="VGT44_09115"
     CDS             129..1019
                     /locus_tag="VGT44_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2581000.1"
                     /translation="MPAIEFGLMLQPFPLDFPGAELFAYNRRLIRTLSPGFTTLWVED
                     HLQLGETATHECLTTLSYFAGEFPQFRVGALVLCQSYRNPALTAKMAANLQWLSGGRF
                     ILGLGAGWKEDEYHAYGYDFPDTKTRLEELEEAAIIIKAMWSSRPATFAGKHYSIRDA
                     YCEPQPDPAIPLLIGGGGEEKTLAIVARRADWWNFNSCTVEEYARKLAILKSHCARIG
                     RNPVEIKLTYLGTASVSEDPGQVVRSPQKHFVAGNSAEVIRELERFHAIGVAQFMFRF
                     LDPASLERFVQTVVPHFYRS"
     gene            complement(949..2274)
                     /locus_tag="VGT44_09120"
     CDS             complement(949..2274)
                     /locus_tag="VGT44_09120"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2581001.1"
                     /translation="MARIVVTTFGSIGDLNPFVALGLALRSNGHDVIFAVEDNFQQQV
                     SLLGFPVCKLTGDQSQALDSYSHQIFNSERPLTSLKLLVDRYILPTLPAKVEELREVC
                     RQADLLISVAPQFAASIVAEMLQIPWMAVILTPSTLPSASIEAQPMSVQLPAPLQRLS
                     NRFSWFLGGMVLRQIVDKPINRVRRAFHVPPRSDLMWTGNLSPRFTAMAVSSALVPRP
                     HDWPEYVRMTGFCFWDGAEDWQFSETLKTFLRGDRPVVAVTAGTVAPGERALFASYYQ
                     TSRESILACGARALLINAPENSDSPEQRENVLQIPFAPFSAIFPMCAAVIHHGGIGTI
                     AQCLRAGVPSLVVPGGIDQPFNAAQVVQRKAGLWIPRKRYTARRAEQALRALLFTPTY
                     KDHTRKIQAQLMQEEGVAILCAEVERIMSDKSAGQPSVRSAPTTQGPET"
     gene            2453..>3127
                     /locus_tag="VGT44_09125"
     CDS             2453..>3127
                     /locus_tag="VGT44_09125"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2581002.1"
                     /translation="MPYSPAVRSETVDLPAGQFHFLSWGAERSELPDMLLLHGITGSV
                     QSWVRVGPELAVHYRVYALDMRGHGESIKPPPGAYSLRHAADDAAAFIEALGLERPIM
                     IGHSWGGATAMVLASGAGSDRPVPALSRLVLEDPAHTFGSGDVEKRAAPYTRDIGRPV
                     EELRAEITASSPGWTEADIEGKIDALHKVSREAVISVFTQAGEAGELLPRLASISVPT
                     LLVRADT"
BASE COUNT          691 a          877 c          905 g          654 t
ORIGIN      
        1 cgcaaaacga ttggatgggc ttcactggct tttggagcat ttaccgccat ttcgatcatt
       61 ggcccgatag ttcttgaacg gctctggtcg cgtcgtcggt aggctttcta tatcgggaga
      121 gttcgtccat gcctgccatc gaatttggtc tcatgcttca gccgtttccc cttgattttc
      181 ctggggccga gctattcgcg tacaatcgcc gcttgattcg cacgttgtcg cctggcttca
      241 ccaccctctg ggtcgaggat cacctgcaat tgggagaaac cgccacgcac gagtgcctga
      301 ccaccctgag ctatttcgcc ggtgaatttc ctcaattccg tgtgggggcg ctcgtgttgt
      361 gccagtcgta ccgcaatccg gccctgacgg cgaaaatggc cgccaatctc cagtggcttt
      421 cgggaggaag gttcattctg gggttgggcg ctggttggaa ggaagacgag tatcatgctt
      481 acggctatga ctttccagac acgaagacgc gcctggaaga attagaagaa gccgccatca
      541 ttataaaggc catgtggtcc tcgcgcccag ctaccttcgc cggaaaacac tacagcattc
      601 gcgacgccta ctgcgagccg caacccgatc cagccattcc gctcctcatc ggcgggggtg
      661 gcgaggagaa aacgctggcg atcgtcgcgc gccgggcgga ctggtggaat ttcaactcct
      721 gcacagttga agagtacgcc cgtaagcttg ccatcctgaa aagccactgc gctcgcatcg
      781 gcagaaaccc ggtggaaatc aaactgacct acctgggtac agccagcgtc tcagaagacc
      841 ccggccaggt agtgcgcagc ccacaaaagc actttgtggc cggcaacagc gccgaagtga
      901 ttcgcgagct ggaacgcttt catgccatag gggtggcgca attcatgttc aggtttctgg
      961 accctgcgtc gttggagcgc ttcgtacaga cggttgtccc gcacttttat cgctcataat
     1021 tcgctcaacc tcggcgcata atatagcaac cccctcttcc tgcataagct gggcctgtat
     1081 tttgcgggtg tgatctttat aagttggtgt aaacaagagc gcgcgcaagg cttgctcggc
     1141 tcgcctcgcc gtgtagcgct tgcggggtat ccacaatccg gctttgcgct gtaccacttg
     1201 tgccgcgttg aagggctgat ctatccctcc aggcaccacc agggacggca ccccggcccg
     1261 caaacactgt gcaatcgtcc cgatccctcc gtgatggatc actgcggcgc acataggaaa
     1321 gatcgcggaa aagggcgcga aggggatttg caacacattt tcacgctgct caggagagtc
     1381 gctgttttca ggggcgttga ttaacagcgc tcgcgcgcca cacgcgagaa tgctttctct
     1441 acttgtctga tagtaggaag caaataacgc ccgttcgccg ggcgcaacag ttccggcagt
     1501 gacagcgaca acgggccggt ctccacgcag aaaggtcttg agcgtttcag agaactgcca
     1561 gtcttcagct ccatcccaga aacaaaaacc cgtcatgcga acatattccg gccaatcatg
     1621 cggtcgaggc acgagcgcag aggagacagc catcgctgtg aaccggggag aaagattgcc
     1681 cgtccacatg agatcggaac gaggcggtac gtgaaacgcg cgtcggacgc ggttaatcgg
     1741 tttgtctaca atctggcgca gaaccatccc tcccagaaac caggagaagc gattgctcag
     1801 gcgttgcaga ggcgcaggta attgcacaga catgggctgt gcctcgattg aagcagaagg
     1861 gagcgttgag ggagtcagaa tcaccgccat ccagggaatt tgcagcatct cggcaacgat
     1921 agacgcggca aactgcggcg cgaccgagat gagcagatcc gcttgtcggc agacttcgcg
     1981 cagttcctct actttggcag ggagggtcgg aaggatatag cggtctacca gcagtttgag
     2041 agaggtcagg ggccgttcgc tattaaaaat ctggtgacta taggaatcca atgcctgcga
     2101 ttggtctcct gttaatttgc aaacaggaaa tcccagcagg ctgacctgct gctgaaaatt
     2161 atcttcaacc gcgaaaatca catcgtgtcc attggagcgc aaggctaatc caagcgcgac
     2221 gaaggggtta agatcaccga ttgaaccaaa ggttgtcacg acgatacgag ccatattgtc
     2281 agtatctcac ctatctcttt atactcgccg gatcaagttg actgcactca gcaataatta
     2341 taggacatca gcgtagctat aggccacctg ttgctgtggc atgggtgtaa gaaatacttg
     2401 tggcataatg aaggtaaaag aatgcctgaa aagcgctcga gaggagagat acatgcccta
     2461 tagccctgct gttcgttctg agactgttga cctgcctgct ggccaatttc attttctgag
     2521 ctggggagcc gagcgcagcg aactgcccga tatgctcttg ctgcacggca tcacgggttc
     2581 cgtgcagagc tgggtgcgcg ttgggccgga gctggcagtc cattaccgcg tctacgccct
     2641 tgacatgcgc gggcatggcg agagcatcaa accgccgccg ggcgcttata gcctgcggca
     2701 cgccgccgat gatgccgccg cgtttatcga agcgctgggg ttggaacgcc ccatcatgat
     2761 cgggcattcc tggggcggag cgacagcgat ggtactggca agcggagccg ggtctgatag
     2821 acccgtaccg gctctctcgc gcctggtcct ggaagacccg gctcacacct ttggatccgg
     2881 cgatgttgag aaacgagccg ccccctatac cagggatatc ggtcgtccgg tcgaagagct
     2941 gcgcgccgaa attaccgcca gcagtccagg ctggaccgag gcagatatcg agggcaagat
     3001 cgacgcgctg cacaaagtca gtcgtgaggc agtgattagc gtcttcaccc aggcaggcga
     3061 ggcgggcgag ctgctgcccc ggctcgccag cattagcgta ccaacgctcc tggtacgagc
     3121 cgacacg
//
LOCUS       DASZAK010000519         2984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_13074, whole genome shotgun sequence.
ACCESSION   DASZAK010000519 DASZAK010000000
VERSION     DASZAK010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2984
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13074"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..1135
                     /locus_tag="VGT44_09130"
     CDS             125..1135
                     /locus_tag="VGT44_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581003.1"
                     /translation="MESNENVQQAAPAAPTRPSDRPPERLVRHVFWKYPLLSVVLLVV
                     VVVLAATIGSPNYPRDTIAGVSNADPGGAILAFTQELDGTASSSGNAQEFGMGDPGQV
                     FVLNPLRQAAPLLGSNTLQQAIATYDGASADQQQTWASNYDKALGTITAMSGGAMSSG
                     IPSPDFSKIDTLKGDFGPIPVLVKTDLQLAQNGYLEQYLQAIDPGHSYHLTNIWLYDH
                     PNMLNTAVALGLTDDQWGMVKERGFSVGPWYLFIPAVFHVYFPGGALGQWFVFWNLLF
                     AAILLFVVPLVPGVRDIPRYLKLYRFIYRYPAPGELERPAFRERHGATHGGEVEAAGG
                     GE"
     gene            1132..1422
                     /locus_tag="VGT44_09135"
     CDS             1132..1422
                     /locus_tag="VGT44_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581004.1"
                     /translation="MNQPQRERQRRGTEFVLFLVASVIGMYGLGQGLTGLFEPGQGIS
                     LPWLAVTGVAVIVLFAQMGRVHDTWPRKPEQPSHPHTNEQAARQAPMQAEER"
     gene            1844..2434
                     /locus_tag="VGT44_09140"
     CDS             1844..2434
                     /locus_tag="VGT44_09140"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV2581005.1"
                     /translation="MLLQANIYKMLVNGDAWGEWQGYQLPVSDEYVTIWSPRGTKRHW
                     KPHKWKPDFFLSERHSLSFFWPGAWHTIHIGYHPDGSFSDGYCDIILPTPAYTSESRE
                     LTYIDLYIDVVIRQDGSVFTKDQEVFDRAALRYPIVEESRQQAYDTLDWVEAHARRWS
                     GPFSVMPRRLPRTDWETLSIKAARAEMRAALQQQQP"
     gene            2431..2673
                     /locus_tag="VGT44_09145"
     CDS             2431..2673
                     /locus_tag="VGT44_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581006.1"
                     /translation="MTLPADIQARLTVHGITSSDEVALREALEARAETYTFIKLAPWP
                     ARRWKCRYRLLLGDTIFDAQTAAEAYALGLLSLLPS"
BASE COUNT          626 a          945 c          827 g          586 t
ORIGIN      
        1 ctggcgatcc acatcgccgt cctgccggtg gtcatcttcg ggctgatcct ggcgcatatc
       61 gtgctgatcc gcatcatggg actcagcaag ccgccgtcga accagaagaa ggagcagtag
      121 catcatggaa agcaacgaga atgtacaaca agccgcgcct gctgcgccca ccaggccgag
      181 cgatcggcca cccgagcgcc ttgtgcggca cgttttctgg aaatatcccc ttctttcggt
      241 cgtactcctg gtagtcgtag tagtgttggc agcgaccata gggtcgccca actatccgcg
      301 agatacgatc gcgggcgtct ccaatgccga ccctggcgga gcgatcctgg ccttcaccca
      361 ggaactggac ggcaccgcct cctcttctgg caacgcacaa gagttcggca tgggcgatcc
      421 cggccaggtc tttgtgctga acccgctgcg ccaggcggct ccgctgctag gatcgaacac
      481 cctccagcaa gcgatcgcaa cctacgatgg agcgagcgct gaccaacagc agacctgggc
      541 ctccaattac gacaaagcgc tcggcacgat cactgcgatg agcggtggcg cgatgagttc
      601 aggcataccc agcccggatt tctcgaagat cgacacactg aaaggggatt ttggccccat
      661 ccccgttctg gtcaagacag atctccaact ggcccagaac ggctacctgg agcagtatct
      721 gcaggcgatt gaccctggac actcctacca cctcaccaac atctggctct acgaccaccc
      781 gaacatgctc aacacggcgg tggcgctggg tctgactgat gaccagtggg gtatggtcaa
      841 ggaacgcgga ttctccgtcg gaccatggta cctgttcatc ccggcagtgt tccacgtcta
      901 cttcccaggc ggcgccctgg gtcagtggtt cgtgttttgg aacctcctgt tcgcggccat
      961 cctactgttc gtcgttcccc tggtgccggg tgtgcgcgac attcccaggt acctcaagct
     1021 ctatcgcttc atctaccgct accccgcccc gggggagtta gagaggcccg ccttcagaga
     1081 acggcacggc gcgacgcacg ggggagaggt agaagcagcg ggaggtggcg aatgaaccag
     1141 ccacagcgag aaagacagcg caggggaacc gagttcgtgc tcttcctggt tgcgtccgtg
     1201 atcgggatgt atggactcgg gcagggtctg accggcctgt ttgagcctgg tcagggtatc
     1261 agtctcccct ggctggcggt cacaggcgtg gcggtcatcg tgctgttcgc ccagatgggg
     1321 cgcgtccacg acacctggcc gcgcaaaccc gagcagccga gccacccaca cacgaacgaa
     1381 caggcggcgc gccaagcgcc gatgcaagct gaagaaaggt gagtgagagg aacatgttcc
     1441 agcgcatcca tcgtgatgtc tactcggtaa tcatcggcct catagtgctg gccgggttga
     1501 ttacactatt gctgaccatc gtcaatccga tacggtaatc gctgctgttt gataagcagc
     1561 agccacaggc atcaaagaaa gcggttcctg cgctgcccct tttcccgact gtcgggcggc
     1621 gcgggaagtc gccttccctg gctcgcgcca ccttccgctt tggcagtttg aacggcaatg
     1681 cttcaaagga gacatatggt gggagccgta tcgcgaagac aagccctgga aagctcgtct
     1741 cctacaactt gtgctatagc ttgacagagc gcttgcctct acccacactc ctcggctata
     1801 atacgttagc aggctatctg ctcaaagaga ggaaaccggg agtttgttac tacaggcgaa
     1861 tatctataaa atgctggtca acggggacgc ctggggcgag tggcagggct atcaactgcc
     1921 cgtcagcgac gagtacgtca ctatctggtc gccgcgcggc acaaagcggc attggaagcc
     1981 gcacaaatgg aaaccagact tctttctctc ggaacgtcac tccctgtcgt ttttctggcc
     2041 cggcgcctgg catactatcc atatcggtta tcaccccgac ggttcattct ccgatggcta
     2101 ctgcgacatc atcctcccaa cgcccgccta taccagcgag tcgcgggagt tgacctatat
     2161 cgacctctac atcgatgtcg tcattcgcca ggatggcagc gtcttcacca aagaccagga
     2221 agtcttcgac cgcgccgccc tgcgctaccc catcgtggaa gagtcgcgcc agcaagcata
     2281 cgatacgctc gactgggtag aggcccatgc caggcgctgg agcggaccgt ttagcgtcat
     2341 gccgcgccgc ctgccgcgta ccgattggga aaccttgagc atcaaagccg cccgcgcgga
     2401 aatgcgcgca gccctgcaac aacagcagcc atgacgctcc cagcagacat ccaggcccgg
     2461 ctgaccgtgc acggcattac ctccagcgac gaagttgccc tgcgcgaagc attggaggcg
     2521 cgcgcagaaa cctacacctt catcaagctc gccccctggc ccgctcgccg ctggaagtgc
     2581 cgctatcgcc tccttctcgg cgacaccatc tttgacgccc agaccgccgc cgaagcctac
     2641 gccctgggac tgctatccct tctcccatcg taggaatata gtacgatcaa ccttcagccc
     2701 tgcaatatcc gtaggcgccg ttttatcgcg cacatcgccg attgatcggc ctggtggcta
     2761 gcgacgcacc tccattgtgg ccaataatga actttcccaa ccatcatcgg ttctgtcatc
     2821 ctgagcgcag cgaaggatct cggtctccag cgcgtgagat cctgagcgca gcgaaggatg
     2881 acagggggaa agtcttgttc ccaaaacgcc attcgacccg ttattataaa taagaggaac
     2941 ttttaccgaa gtatccttta cgggccctac atcactgtag accc
//
LOCUS       DASZAK010000520         1707 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_47278, whole genome shotgun sequence.
ACCESSION   DASZAK010000520 DASZAK010000000
VERSION     DASZAK010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47278"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /gene="hemC"
                     /locus_tag="VGT44_09150"
     CDS             complement(<1..1685)
                     /gene="hemC"
                     /locus_tag="VGT44_09150"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEV2581007.1"
                     /translation="MKKSITIGTRASKLAMIQTRSVIERLQQSWPGLEVKIEQIHTRG
                     DLNTEAPLTQIGSDGVFVTEIERALQDGRIDLAVHSLKDLPTAQPVGLTIAVVGPRED
                     VRDAMVSRAGGQSSAQDFQEQLARISEPLRIGTCSLRRTAQIRRLCPDAQILPLRGNV
                     DTRLRKLDAGEYDAIVLAAAGLHRLDLPSRLAGRVSYFPIETLMPAPGQGALAVEIRD
                     EPDMRELIQSIVNRDAQAATSAERMFMRRLGAGCYLPVAAHGEINGETLTLRGLVISL
                     DGQRHVRVQQRIRWTAQTSIECAEQLGVTLAEEALAQGADEIIRELGIRGNTGDGING
                     KRILVTRSGEQASALSDRLRALGAIPIELPLIRIDPPEDWQPLDEALRRLCEHGEQPE
                     PPYYAWLVFTSANGVTHFFGRLRAQGYDVQTIRGTRVAAIGSATQAALARQGISVDLV
                     PGEYAAEELVAALVEDARKQHETLQGKRVLLARAAEARNVLPLGLQQEGALVDDVAVY
                     RTSPTARDDETGREALSLLQTGRLDMLTFTSSSTVRNFVQWLGDLIRQNPHMKV"
BASE COUNT          301 a          561 c          503 g          342 t
ORIGIN      
        1 accttcatgt gtgggttttg acgaatcaag tcgccgagcc attgcacaaa attgcgcacc
       61 gtcgaagagc tggtgaaggt caacatatcc agccgaccgg tttgcaacag gctcagcgct
      121 tcccggccag tttcgtcatc cctggcagta ggtgacgtgc ggtagaccgc tacgtcatcg
      181 acaagcgcgc cttcctgctg caagccgagt ggcaggacgt tgcgcgcctc ggcagcgcgg
      241 gcaagcagca cacgttttcc ttgcagggtt tcatgctgct tcctggcgtc ctcgaccagc
      301 gcggcaacca gctcctccgc tgcatattcg cccggcacga gatcgacgct gatgccctgg
      361 cgcgccaggg ccgcctgtgt tgcagacccg atggccgcca cacgcgtacc gcgtatagtt
      421 tgaacatcat acccctgcgc gcgcaatcgc ccaaaaaagt gggtgacgcc attggcgctg
      481 gtaaacacca gccaggcata atacggcggt tccggctgct ctccatgctc gcagagacgc
      541 cgaagcgcct catcgagcgg ctgccaatct tccggtgggt cgatgcgaat cagtggcaac
      601 tcgatgggga tcgcgcccaa tgccctcagt ctgtcgctca gcgcgcttgc ctgctcgccg
      661 gagcgtgtca ccaggatgcg cttaccgttg atgccatctc ccgtgttccc tcggatgccc
      721 aattccctga tgatctcgtc agcgccttgt gccagcgcct cttccgccag ggttacgccc
      781 agttgttcgg cgcattcgat gctggtctgg gcggtccaac ggatgcgctg ctgcacgcgc
      841 acgtggcgct gcccatccag gctgatgacc agcccgcgca gcgtgagcgt ttcgccgttg
      901 atctctccgt gcgccgccac gggcaggtag cacccggccc ccagacggcg cataaacatc
      961 cgctcggcgc tggtggctgc ctgcgcatct cggttgacga tggattggat cagctcgcgc
     1021 atgtcgggtt cgtcgcgtat ctcaacggcg agcgcgccct ggcccggcgc gggcatgagg
     1081 gtctcgattg gaaaatagct gacgcgccct gctaacctgg atggcagatc gagccggtgc
     1141 agtccggctg ccgccagcac aatcgcatcg tattcgcccg cgtcaagctt gcgcaggcgt
     1201 gtatcaacat tgccccgtaa ggggagaatt tgcgcgtcgg gacagaggcg gcgaatttgc
     1261 gcagtgcggc gcaggctgca cgtgccgatg cgcagcggct cggaaatgcg cgcaagttgc
     1321 tcctgaaaat cttgcgcgct gctctgccca cctgcgcggg acaccatcgc atcgcgcaca
     1381 tcctcgcgcg ggccgacgac agcgatggtc aggccaacag gctgcgcggt gggtaagtct
     1441 ttcagactat gcaccgccag atcgatacgc ccatcctgca gcgcgcgctc gatctcggtc
     1501 acaaacacgc cgtcgctgcc gatctgcgtt aacggcgctt cggtattcaa atcgcccctg
     1561 gtgtgaatct gctcaatctt cacctccagt ccaggccatg actgttggag acgctcgatg
     1621 accgagcgcg tctgaatcat cgccagcttg ctcgcccgcg tcccaatcgt aatactcttc
     1681 ttcatgttgt acttccagat caaagat
//
LOCUS       DASZAK010000521         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_118835, whole genome shotgun sequence.
ACCESSION   DASZAK010000521 DASZAK010000000
VERSION     DASZAK010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118835"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VGT44_09155"
     CDS             complement(<1..1314)
                     /locus_tag="VGT44_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV2581008.1"
                     /translation="MELELRINGLIKSLEVAPNESLMAVLRKEGYFSVKHGCETGECG
                     ACTVQVDGVPRPSCVTLAAQVGGCTLTTVESLGTARKLHPIQEAFVDTGAIQCGFCTP
                     GMVLSAHALLKRNPNPTEDEVRDALSGNLCRCTGYVKPVQAILRAAAILRGEEMAPIS
                     RPNDDENFQPWDPGRSPLEHAEHGASGEKGHPSHEEDSPGGGTATLVDTRVSTDLSVV
                     GKAERKVDAVKLVTGKPAFVDDIDLRGLLHGRLLTSPHAHAIIREIDVSRARALPGVH
                     AVLTYKDVPRIPYTTAGQSWPEPGPHDQYCLDYRVRFVGDRVAAVAAETPEIAEQALK
                     LIEVDYEILPALLDPRKAMDPDAPRLHPEPESYRIHDASRNIAAHLHAEVGDVERGFA
                     ASDLVVEGEYIVPQVQQTPIENHIVITYWDEDDRLVVRTSTQVPYH"
     gene            complement(1317..2303)
                     /locus_tag="VGT44_09160"
     CDS             complement(1317..2303)
                     /locus_tag="VGT44_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV2581009.1"
                     /translation="MLLNLLEYHWVEDIDDALLLLARADAKTVPLAGGTYLLGLEDEN
                     IRAVVDLRDLELAYITEDAKGIRVGAMTTLQTMGDSPLLKELVTGIVARASIASSFSR
                     LIRESATLGGTLAAGVASQADLLTALAVMDAEVVLRSGSKSQINLSGGTAERPGLALS
                     GVTFKGKQERRVACSALNRERKPNELIIEVIIPRPASGCAASFTRIGRTPTDVALLNA
                     AALVEVNGDVYQRVRLALGGVHMEPMRMEAVEKQLEGQPVADPGSPNNALNTQRVVDA
                     VQAGMANFSPPGDFRASSGYRRVSGMSLAFHVLEEAANISRWRNMVSSEGRQ"
     gene            complement(2389..>3264)
                     /locus_tag="VGT44_09165"
     CDS             complement(2389..>3264)
                     /locus_tag="VGT44_09165"
                     /inference="COORDINATES: protein motif:HMM:NF024774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2581010.1"
                     /translation="IDSMVRRAIELGLDPVEAIRLATYNTAQYFRLYDRGTVAPNFVA
                     DFVVLDDLSTFKVESVYKDGKLVAQDRKLLVEVPTVTFSGITGTVHMGNISENDLRIP
                     GKAGLIEVVGIEAGQITTTHLREEAPLRDGEIVADPARDLLKLVVIERHHASGNVGLG
                     MVKGFGLRKGALASSVAHDAHNLVIAGVNDGDILHAAKVLQEIGGGFACVVDGEVRAK
                     VPLPYGGLVSPLSANELVQQLHVLDTAAAELGCTLEHPCMTLSFLSLSVIPSLKLTDQ
                     GLIDVETFKLVPLQS"
BASE COUNT          761 a          960 c          819 g          724 t
ORIGIN      
        1 atggtagggt acttgcgtac tggtccttac cacgagacga tcatcttcat cccagtaggt
       61 aatcacaata tggttttcaa tgggagtctg ctgcacctgt gggacaatat actcgccttc
      121 aacgacaagg tcagaagcgg caaagccgcg ttccacgtca ccaacctccg cgtgcaagtg
      181 cgctgcgatg ttgcgcgagg catcgtggat gcggtatgac tctggctctg gatgcaaccg
      241 tggggcatca gggtccatgg ccttgcgtgg gtcaagcagc gcaggcaaga tttcgtaatc
      301 gacctcgatc agctttaacg cttgctcggc gatttcaggc gtctcggctg caacggctgc
      361 gacacgatcg ccaacaaaac gcacccggta atcaaggcag tactggtcat gcgggcctgg
      421 ctcgggccag gattgcccgg cagtcgtgta tgggatgcgc ggcacatctt tataggtcaa
      481 cactgcatgc acaccgggta gggctcgcgc ccgggaaaca tcgatctcgc gaatgatggc
      541 atgcgcgtgg ggactggtga gcaaacgccc gtgcaacaag ccacgtaagt caatgtcatc
      601 gacaaaagca ggcttacccg tgaccagttt gacggcgtct accttgcgct cagctttgcc
      661 aacaacgctc aaatcggtgg aaacacgcgt atcgaccagc gttgcagtcc caccgcccgg
      721 cgaatcttct tcgtggcttg gatggccttt ttctccacta gcgccatgct ccgcgtgttc
      781 taaaggagag cgcccaggat cccatggttg gaaattctca tcatcattgg ggcgactaat
      841 cggcgccatc tcctccccgc gcagaatggc agcggctcgc agaatagcct gcacaggttt
      901 gacgtagccg gtacagcggc acagattgcc tgaaagcgca tcgcgcacct catcttccgt
      961 tggattggga ttgcgtttga gtagcgcatg agcagacagt accatacccg gagtgcagaa
     1021 cccacactga atagcgcctg tatcgacaaa ggcctcttgt atgggatgca gtttacgtgc
     1081 agtacccagg ctctcgacgg tggtcaatgt acagccgcca acctgcgctg ccagggtaac
     1141 gcagcttgga cgcggcacgc catcaacctg cacggtgcag gcgccgcatt cgccagtctc
     1201 acaaccatgc ttgacgctga aatagccctc tttgcgcagt actgccatca gtgattcgtt
     1261 aggcgcaact tccaggctct tgataagccc gttgattcgc aattcgagtt ccatagctat
     1321 tgcctcccct ccgatgaaac catattgcgc caacgtgaga tgttagcggc ttcttcaagc
     1381 acatgaaacg ccaggctcat accactcacg cggcgatagc cactgctcgc gcgaaaatct
     1441 cccggtggcg aaaaatttgc catacctgct tgtaccgcgt caacaacacg ttgcgtattt
     1501 agcgcattgt ttggactccc tggatcagcc acgggctgac cttcaagctg tttttcaacg
     1561 gcctccatgc gcattggctc catatgtact ccacctaaag ccagccgcac tcgctggtag
     1621 acatctccgt ttacttcaac gagcgcggcg gcgtttaaca gagcgacatc ggtgggagtg
     1681 cgcccaatgc gcgtgaatga tgcagcacaa cctgaagccg gacgcggaat gatcacttca
     1741 ataatcagtt cgtttggctt gcgctcccta tttaatgcgc tacaggccac acgccgctcc
     1801 tgtttgcctt tgaaggtaac acctgacagc gcaagcccgg gtcgctcggc tgtgcccccg
     1861 cttagattga tttgcgattt ggagccggag cgcaacacga cctcagcatc cattaccgcc
     1921 aatgctgtaa gcaggtcagc ctgtgacgcg acacccgcag ccagtgtgcc accaagagtc
     1981 gcgctctcgc gaatcaagcg cgaaaacgat gaggctattg aggcgcgtgc cactattccc
     2041 gtaaccagtt ctttgagcag tggagaatcc cccatcgttt gcagggtggt catcgcgcca
     2101 acacgaatgc ccttcgcatc ttcggttata tatgcaagtt cgagatcgcg caaatcaacc
     2161 acggcccgga tattctcatc ttccagacca agcaggtagg ttccacccgc caacggaaca
     2221 gttttcgcat ctgcgcgtgc caataacaat agcgcgtcgt caatatcttc cacccagtga
     2281 tattcaagta gattcaataa catgatattc accttcagtt cttagcatct tatcatcaag
     2341 ccgcctgaaa tgtaggggca tccgtcgaag atgcttactc taccagatct atgattgtaa
     2401 tggtaccagc ttgaacgttt caacatcgat gagtccctga tcggtcagtt tcagcgatgg
     2461 aataaccgat agcgagagga aactgagcgt catgcaggga tgttccaatg tgcagccaag
     2521 ctcggcagcg gctgtatcca gaacatgcag ttgctgcacc aattcattgg cagataacgg
     2581 cgagacaagg ccgccatatg gcaacggcac cttggcgcgc acttctccat ccactacaca
     2641 cgcaaaacca ccacctatct cttgcagaac ttttgccgcg tgcaaaatat cgccatcgtt
     2701 cactcccgcg atcaccaggt tgtgcgcatc atgagcaacg ctggaagcca gcgcgccttt
     2761 gcgcaaacca aaccccttga ccattcccag accaacatta ccgctggcat gatgccgttc
     2821 gatcaccacc agtttgagca gatcacgagc agggtccgcc acaatttctc cgtctctcaa
     2881 gggagcctct tcccgcaagt gcgtagtggt aatctgtccc gcttcgatcc caaccacctc
     2941 gatcaatcca gctttcccag gtatgcgcaa atcattttca ctgatgtttc ccatgtgcac
     3001 ggtgccggtg atgccgctaa aagtaacagt aggaacctcg accagcaact ttctatcctg
     3061 ggcaaccagt ttgccatcct tataaactga ctcgacctta aatgtgctga ggtcatctag
     3121 taccacgaaa tcggcaacaa aattgggtgc aactgtgcca cgatcataca agcgaaaata
     3181 ctgcgcggta ttgtaggtgg cgagacgtat cgcctctacg gggtcaagtc ccaattcgat
     3241 ggcgcggcgc accatcgagt caat
//
LOCUS       DASZAK010000522         4090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_545155, whole genome shotgun sequence.
ACCESSION   DASZAK010000522 DASZAK010000000
VERSION     DASZAK010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545155"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VGT44_09170"
     CDS             complement(<1..959)
                     /locus_tag="VGT44_09170"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2581011.1"
                     /translation="MYTYPFSAIVGQEDMKLALILNAIQPAIGGVLIRGEKGTAKSTA
                     VRALAAVLPELAVVSDCPFSCPPDQPAAMCASCRTRLEQGESLPISHRRMRVVDLPIN
                     ASEDRVVGTLDLEHALREGQRRFEPGVLADANRGILYVDEVNLLDDYVVDVLLDAAAM
                     GVNIVEREGVSFSHPARFMLVGTMNPEEGELRPQLTDRFGLCVDITSIHNIQQRVEIV
                     ELREGFEATPQKFLSGYAGAEQELRSRITRAAQLEKLIIVPREVAELIALINIELDVD
                     GHRGDIVMRRAAQTYAAYLGVEEVRPEHVYTIAPMALAHRLKRL"
     gene            complement(1054..2163)
                     /locus_tag="VGT44_09175"
     CDS             complement(1054..2163)
                     /locus_tag="VGT44_09175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEV2581012.1"
                     /translation="MKTRTAVLYAPGEAIRVEEVELDPPKEHEVQVKIVAAGVCHSDY
                     HMVTGELPGYMPMALGHEGAGIVEEVGSAVTNCKPGDHVVLSFIPSCGTCYYCTRGHT
                     NLCNMGASILMGPQLDGTFRMHNAQGQDVGQLCVVSTFSERTVVSDMSVVKVADYYPL
                     NKAVLVGCGVPTGIGAVIHRAKVEPGSTVMVIGCGGIGMNAVQGAHIAGARMVIAVDV
                     ADFKLEKAKDFGATHTINPKNQDVVQTVKDLTWGMGVDYAFEAIATPATIGQAYACLG
                     KNGTVVVIGLTPATAESIPIPPLDLVLFQKSIMGTLYGDSQPRNDIPNLLAMYHAGKV
                     KLDELVTRTYTLDQINEAYADMLAGKNIRGVIEFA"
     gene            complement(2270..>4090)
                     /locus_tag="VGT44_09180"
     CDS             complement(2270..>4090)
                     /locus_tag="VGT44_09180"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2581013.1"
                     /translation="PPLTGVADCPYFCDPTAPQWMCHDCQARYEREEAFPIAQRPVHL
                     ITLPLNASEDRVAGSFDLATALASGLSHFSPGLLAAANRGFLYVDEINLLNDHIVDLL
                     LDAAALGVNRVERDTISLAHPARFVLIGTMNPEEGLLRPQLLDRIGLYVEAEDQRDPA
                     NRALVVERRLAYDASPYEYITLWSEAEQALLERIKHAQQLLPSVQVSRYFRQVVATLV
                     DRQLHAEGHRGDLVVLRTARALAAWEDRTEVQLSDIEQAVGLALPHRARAGAAIGQHL
                     TQLFDQLPTATTDVDIPEEIATIDDDIDVDKRGDEREGAAGLSRRLRGDQDVEQVHTL
                     ISVTRDRLARQEAGRRFLSLTTRRSGRFTRSRQANPVTDVAFSATIRAAAPYQQVRGR
                     IEGSKLELRPDDLRQKVRERKTRSLLVFVIDGSDSVMARHFMPKTKRILLALLQNAYE
                     KRDRVAMLVFRFAQGRVVLPPTRNFARARRSVEELYIGGCTPLATGLLEGLRLAETER
                     LRDPTTRPILIVLTDGLANIDLSGNMRHDTPQRDALNVAQEIATRQIPAVMIDTNPYP
                     TVTNEAGEKVPGPGRELAQALAADYYQYCLPHETLLLRKG"
BASE COUNT          914 a         1087 c         1153 g          936 t
ORIGIN      
        1 aggcgtttga gcctgtgggc cagggccata ggcgctatgg tgtagacatg ttcgggtcgg
       61 acttcttcca cgccaagata agctgcgtag gtttgtgctg ccctgcgcat cacgatatct
      121 ccacgatggc cgtctacgtc aagctcaatg ttgatgagcg caataagttc agcgacttca
      181 cggggaacga taattagctt ttccaattgg gcggctcgtg tgatgcggga acgcagttcc
      241 tgttcagcac cagcataccc ggagagaaac ttctggggag tggcctcaaa gccttcgcgc
      301 aactcgacga tctcaacacg ctgctgaatg ttgtgaatgg aggtgatatc gacgcagagt
      361 ccaaagcgat cggtcagttg cggacgcaac tcgccttctt cgggattcat tgtgcctacc
      421 agcatgaagc gtgccgggtg actgaacgag accccttcgc gttcaacaat gttgactccc
      481 atcgcggcgg catccagcag cacgtcgaca acataatcat ccagcaagtt tacttcatcg
      541 acgtagagaa taccccggtt tgcatcggcg aggactcccg gttcgaagcg tctctgtccc
      601 tcgcgcagcg cgtgttcaag atcgagtgtg ccgacaacgc gatcttccga agcgttgatg
      661 ggcaaatcga caacacgcat gcgacgatgg cttatgggta gcgattcgcc ctgttccaag
      721 cgagtccggc aggaagcgca cattgccgct ggttggtcag gaggacagga gaaaggacaa
      781 tcactgacta ccgccagttc cggcaacacg gcggcaagcg ctcgtactgc tgtcgattta
      841 gctgtacctt tctctccacg tatgagtacg ccgccgatgg ccggttgaat ggcattgagg
      901 atgagggcca gtttcatgtc ttcctggcct acgatcgcgc tgaatgggta tgtatacatg
      961 atagcctatc tattaattgc cacaccttcc tatgctaagc tgcatacata ggaaggtgtg
     1021 gcgctcaaga cgcaatactg tgcctcgctt tggttacgcg aactcgatca cgcctcggat
     1081 gttcttgcct gcgagcatgt cggcataggc ctcgttaatc tgatcgagcg tgtacgtcct
     1141 ggttactaac tcgtccagtt ttacttttcc tgcatggtac atcgcaagca ggtttggaat
     1201 gtcgttgcgc ggctgagaat cgccataaag cgttcccata atcgacttct ggaaaagcac
     1261 gagatcaagc ggtggtatcg gaatagattc ggcggtggca ggggtcaggc cgatcacgac
     1321 tactgtacca tttttgccta gacatgcata ggcttgacca atagtggcag gtgtggcaat
     1381 agcctcaaaa gcgtagtcca cacccatacc ccacgtcaga tccttgacag tctgaacaac
     1441 atcctggttt ttcgggttga tcgtgtgagt tgccccaaaa tctttcgcct tctccagctt
     1501 gaagtcagct acatcgacag cgataaccat gcgcgcgcca gcaatgtgag ctccctggac
     1561 agcgttcatg ccgatgccgc cgcagccaat gaccatgaca gtacttcctg gttcgacctt
     1621 cgcgcggtgg ataaccgcac caatgccagt tggcacgccg caaccaacga gtaccgcttt
     1681 gttgagcgga taatagtctg caaccttgac caccgacatg tcagatacaa cagtgcgttc
     1741 gctgaaggta gagaccacgc acaactggcc cacgtcctga ccctgcgcgt tgtgcatgcg
     1801 gaaggttccg tcaagctggg gacccatcaa gatagaagct cccatattgc agagattggt
     1861 atgacccctt gtgcaatagt agcatgtacc acacgagggg atgaagctga ggacgacgtg
     1921 atcacctggc ttgcagttgg tgaccgcgga accaacttcc tcaacaatgc ctgctccttc
     1981 gtgaccgagc gccattggca tgtagccagg tagttcgccc gtgaccatgt gatagtcaga
     2041 gtggcagacg ccggctgcga ctatctttac ctgtacttca tgctcttttg gtggatctag
     2101 ctcgacttct tcgacgcgga tggcttcgcc tggtgcgtac aaaacagcgg ttcgtgtctt
     2161 cattgaatac gacctttctt ctggtgagcg tttgctctaa atagctacct tgctgtaaat
     2221 tgataggaga cggcccgttt cagacaaaga aaatacggtg tgtagtttct cacccctttc
     2281 tcagtagtag agtttcatgt ggcaggcaat actggtaata gtcggcggcc agggcctgag
     2341 ccagttcgcg tcctggacct ggtaccttct cgccggcctc gtttgtcaca gttgggtatg
     2401 ggtttgtatc gatcataacc gcgggtatct ggcgagttgc gatctcctgg gcgacgttga
     2461 gggcgtcacg ttgtggagta tcgtggcgca tgttgccaga gagatcgata tttgccagac
     2521 catcggtcag gacgatcaag atgggcctgg tggttggatc acgaaggcgt tccgtttcag
     2581 cgagacgcaa gccctctaac aagcctgttg ccagcggcgt gcagccaccg atatagagtt
     2641 cctcgacact gcggcgagcg cgtgcgaagt tgcgtgtggg tggcaggacg acgcgaccct
     2701 gggcaaagcg aaagacaagc atggcaacgc ggtcgcgctt ctcataggcg ttttgcagga
     2761 gtgccagcag gatacgttta gttttgggca tgaagtgtcg ggccatcacc gaatcgctgc
     2821 catcaatcac gaagacgagc agcgaacgag ttttgcgctc gcgaactttc tggcgaaggt
     2881 catctgggcg cagttccaat ttgctccctt cgattcgccc acgcacctgt tgatagggag
     2941 ctgctgcccg gatggtagca gagaaagcaa catcagtgac cgggttggcc tggcgactgc
     3001 gagtgaagcg tccgctccgc cgcgttgtca gcgagagaaa acgtcgccct gcttcctggc
     3061 gtgccaggcg atctcgagtg acagagatga gtgtatgtac ctgctcaaca tcctgatcgc
     3121 ctcgcagccg cctgctgaga ccagcagcac cttcacgctc atcgccacgt ttgtctacat
     3181 cgatatcatc atcaatagta gctatctctt ctgggatatc tacgtccgtg gtcgctgtag
     3241 gcaattgatc gaaaagctgt gtaaggtgtt ggccgatggc agccccggcg cgtgctctat
     3301 gaggaagagc gagacctaca gcctgttcta tatcggagag ttgtacttct gtgcgatcct
     3361 cccaggcagc taaagcacga gcagtccgca gcacaacaag atcgccgcga tgcccctcgg
     3421 cgtgcaattg gcgatcaacg agcgtggcga caacctggcg aaaatatctg gaaacttgta
     3481 cggatggcag cagttgctgg gcgtgcttaa tgcgctcgag taatgcctgc tcagcttctg
     3541 accaaagcgt aatatactca taaggagatg catcgtaagc aagacggcgt tcaaccacca
     3601 gtgcccgatt ggctggatcg cgctggtctt ccgcctcaac atagagtccg attctatcga
     3661 gcaactgagg gcgtaacagt ccctcttctg gattcatggt gccgatcaac acgaaacgcg
     3721 cgggatgagc aagcgagatg gtatcccgct cgacacgatt gacccccagc gcggcggcgt
     3781 caagcaatag atcaactata tggtcattga gcaggttgat ctcatcgaca tagagaaagc
     3841 ctcgattggc tgctgctaac aaccctgggg agaagtgact gaggccgctt gccagggcag
     3901 ttgcaagatc gaagctccca gcgacacgat cttcgctggc attgagtgga agggttatca
     3961 gatgtacagg tcgttgggca atggggaacg cttcttcgcg ttcataacga gcctggcagt
     4021 cgtgacacat ccattgcggg gcagtcggat cgcagaaata aggacaatca gctacgccag
     4081 ttaggggggg
//
LOCUS       DASZAK010000523         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_545170, whole genome shotgun sequence.
ACCESSION   DASZAK010000523 DASZAK010000000
VERSION     DASZAK010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545170"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VGT44_09185"
     CDS             complement(<1..799)
                     /locus_tag="VGT44_09185"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEV2581014.1"
                     /translation="MRLGRHMPTHSNAVKAAELARSIGCNAIQIFASNPTGWRPPSDN
                     GASSIAFAQAARAHDLDPVVIHAPYLINLASPDDLIWEKSIALLRWTLQRGALLGACY
                     VVFHTGSHRGAGVEVGIARIAEAIRRILPETPPEVILLLENDVGAGYSVGHSFEQLRA
                     ILALLPEYRERLGVCIDTAHLWGAGHDISTAASTLQVLAHIDATFGLDCLKVIHLNDT
                     EMALGSHRDVHARLGEGIIGEEGLRALLQDSRLAHAAVILETPIQTNE"
     gene            complement(804..1418)
                     /gene="scpB"
                     /locus_tag="VGT44_09190"
     CDS             complement(804..1418)
                     /gene="scpB"
                     /locus_tag="VGT44_09190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEV2581015.1"
                     /translation="MLQELQERHSVTKVAAAIESILFVSGRPLEYGELRRLLDIDDVA
                     LNEGLEALAEQLERQERGIRLQRLGMQVQLVTAPENARYIAALLGLPMTAKLTNAALE
                     TLSVIGYRQPITRAQIEAVRGVNSDRALLSLIQHGLVAEIGRAQTVGRPALFATTPEF
                     LQQFGLTNLDQLPRSALVAEPSGAPDAQKAQETRDAIATPEERN"
     gene            1975..>2115
                     /locus_tag="VGT44_09195"
     rRNA            1975..>2115
                     /locus_tag="VGT44_09195"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          450 a          582 c          651 g          432 t
ORIGIN      
        1 gctcgttggt ctggatgggc gtctccagaa ttacggcggc gtgtgccagg cgcgagtctt
       61 gcagcagcgc gcgcaggccc tcttcgccga tgatgccttc tccgaggcgc gcgtgaacat
      121 cgcggtggct gcccagggcc atctcggtat cattgaggtg gatcaccttc aagcaatcta
      181 agccaaaggt agcgtcgatg tgcgcgagta cctgaagcgt ggacgcggcg gtggagatgt
      241 cgtgtcccgc tccccatagg tgagctgtat cgatgcagac ccccagccgc tcgcggtatt
      301 ccggcaagag ggccagaatg gcgcgcagct gctcaaagct gtgtccgacc gaatagcctg
      361 ctcccacgtc gttttcgagc agcagtatga cttccggcgg cgtttccggc aagatgcgcc
      421 gaatagcttc ggcgatgcgc gcgatgccga cctctacgcc cgcgccgcga tggctgccgg
      481 tatggaagac gacatagcac gctcccaaca acgcgccgcg ttgcaatgtc cagcgcaaga
      541 gcgcgatgga tttttcccag attaggtcgt cgggcgaagc caggttaatc aagtagggtg
      601 cgtggatgac gaccgggtcc agatcatggg cgcgcgctgc ctgggcgaag gcgatgctgc
      661 ttgcgccgtt atcggatggt ggtcgccagc cggtggggtt gctggcgaag atctggatgg
      721 cgttgcagcc gatggagcgc gcaagctcag ctgctttgac ggcattggag tgtgtgggca
      781 tgtgtcggcc gaggcgcata gcgtcagttc ctctcttctg gcgtcgctat cgcgtcgcga
      841 gtctcttgtg ccttttgcgc gtccggcgct cctgatggtt cagcaactag ggctgatcgg
      901 ggtaactgat cgaggttagt caaaccaaac tgttggagaa attcaggtgt ggtggcaaaa
      961 agcgcgggcc tgccgaccgt ctgcgcgcgt ccgatctcgg ccaccagccc atgctggatc
     1021 aaactgagaa gggcgcggtc gctgttcacg ccgcgcacgg cttcgatctg ggcgcgcgtg
     1081 atgggctggc gatagccgat caccgagagc gtttccagcg ccgcgttggt cagcttcgcg
     1141 gtcatgggca gccccagcaa tgcggcgata tagcgcgcat tctccggcgc agtcacgagc
     1201 tgcacctgca tccccaggcg ctgcaagcgg ataccgcgtt cttgccgttc aagctgctcg
     1261 gccagcgcct ctaacccttc gttcaacgcg acatcatcga tatcgagcaa cctgcgcaac
     1321 tcgccatact ccaacgggcg accagagaca aaaaggatgc tctcgatagc tgccgcaacc
     1381 ttcgtaacgc tgtgcctttc ctgtaattcc tgaagcattt gttcctacct tgctattcta
     1441 aacaggagtg tagcatatcg gtaaaatgct gtcgagtccc ttttgtacga cgcgggatca
     1501 gataaaaact tgtccggtca tatcgagaaa atcaagacaa aaactattga caaagcgtta
     1561 aggctatggc ataatacgaa agtcgccttg agcgacaggg aacaacgtct ggtatgcaaa
     1621 cccccaaaaa ggtttctccc aagaattaac gaacccaggg ttcatctgtt ccttttgggg
     1681 gaacagatag tctggttagt ttctctgtct ctaaacggcg atgcagaaat gggttagcgc
     1741 ctatttcata gggtaccttg acaactgaag agtggaatgc agaacgaacg actggagagg
     1801 accgcgtcgg aacgcccgac gagggaagcg cggggtcggc tcctgtcaat tcacgaacca
     1861 attgagaatc tgcgaacaag ccatgcgaac atcacgaatg gacaagtggc ctggatcgcg
     1921 cgcggacatc agcgcttgag ccgcctcccg caggggaagc ggccacagat atgatggatg
     1981 gcgagtttga tcctggctca ggataaacgc tggcggcgtg cctaacacat gcaagtcgaa
     2041 cgctctttct tcgcaaggag agagggagtg gcggacgggt gagtaagaca tgggaaccta
     2101 ccccatcagt gaggg
//
LOCUS       DASZAK010000524         4745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_404296, whole genome shotgun sequence.
ACCESSION   DASZAK010000524 DASZAK010000000
VERSION     DASZAK010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404296"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /locus_tag="VGT44_09200"
     CDS             complement(<1..1186)
                     /locus_tag="VGT44_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581016.1"
                     /translation="MVRSLDSALTNALNGATRVPVIRLVAEDRVAHYAQYQTPGSADA
                     WHDACIANDYSIVRVQLPRGGSGFANAFQVQRITDPSQASQWSAWTNLPGSSGSMFQD
                     GGCAISNNAGTLRAFAQRGTGGNTLWAWTSSNNGVTWSGPVGVLSPPGGALTKGIGSG
                     GNNDVFFLYDVSGGEAMGCSFYNGSSWSALNTWSLPTIAYGAGVAVAWVNSQYTIVYS
                     DSYSLFSCIYNPSGGTWSSSAVIAPSTSTAIGRFSPRLSYDGYLYTLTCVEADTGALT
                     GSVYSYPRLRQSLDLLHWSNGFILHDLSCSYGATSFKLTMPYSGSSGARYYVVGMPTV
                     FSAPVFSTLNANQYLDLSASVLSYKRQEEPGKPARLDILVDNARGLYDTLLTSGSTYQ
                     PVG"
     gene            1506..2645
                     /locus_tag="VGT44_09205"
     CDS             1506..2645
                     /locus_tag="VGT44_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581017.1"
                     /translation="MLSLARVARFPVKRLVLSSAFLLLAFASMFANTQVQARGKTSPP
                     GKEINPATVMGVQVNGPSSLPGISWVRLGYATCGAGDPSGAALQGIIDGYHRHGVHVL
                     LTVCQPKPAHLLDTGYLNDAAQANPDAVQCGNEEMKGGAYNIYISPQLFAAFYDLCQK
                     AVHAVNPSAVIVLGSLDPHVARYDTQLLLGEVNYLNAMQEAMNARVYKHGHWTWRSQI
                     VGLINSWHDGYPSLYINNLRDLYTFWAWAFHVDMNQLGNHLWVVEDTGCFKGCGVPDN
                     QRQIAIAHILALIVDVRVTMEYRVPFFFFSARDFFASGAYWPIGILNNKGQPKPLRQD
                     LPMGSRSLLMSCGKLKTRVITQEQLLATMYYGCQLPENSYNTLIY"
     gene            2786..3973
                     /locus_tag="VGT44_09210"
     CDS             2786..3973
                     /locus_tag="VGT44_09210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2581018.1"
                     /translation="MAEVEQHRRLQIAYLTAEDPQSKYAWSGTTYYMAQALQKHCGDV
                     HYLGPIMSFERRVIGRLMHEASRKALGKNIAYDRLPFVAKKQAKIASQRLASGAFNLI
                     FAPIGPPEVALLETDIPIILALDITFALQHNYHERYSALLPWSVKMSNKIEEMAYQRA
                     SALIYPGRWSARSAIEDYHVDSQKVHIVPFGANLDVPPNGKIVRNRRRSHRCRLLFMG
                     LGWERKGGDIAFETLLKLEEMGIEAELIVCGNTPPAGLSHERMKVIPFLNKHDEKQYE
                     ELEQLYTTSDFLLLPTRCDCFPIVFCEASAFGLPVVTAHTGGVSDAVLNGINGYVLPY
                     EARGADYARVIAELFRDETRYAKLVESSRAMFENKYNWDVWGVTMNTILQHMAAPVPA
                     YIP"
     gene            complement(3932..>4745)
                     /locus_tag="VGT44_09215"
     CDS             complement(3932..>4745)
                     /locus_tag="VGT44_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581019.1"
                     /translation="NSSTCQLYELWDAFPQPGGSWTAGSGATWNLNSDALRPLYWTSA
                     DAAGLPILPGLVRYDEIAAGSINHALRVVANATQMAFLWPARHYASSSTDPNLPPMGL
                     RLRLKASFDISSFPRDVQVILTALKRYGMFVADNQGVTSWVLTGAPDSRWNNFDLNSL
                     GKVPGSAFEAVNESMLQTAPDSARVIARALPHTPVTFVSVQKTFSSYQPVAFDNIIDI
                     RKVKLEPRQPDNVYIITDDVCGLAKQRMESSKDSLLLEILRNICWYRGSHML"
BASE COUNT         1127 a         1257 c         1307 g         1054 t
ORIGIN      
        1 gcccaactgg ctgataggtg ctgcccgaag ttagcaaagt atcatacagg ccgcgcgcgt
       61 tatcgaccag gatgtccagg cgcgcaggct tgccaggctc ctcctggcgc ttgtacgaaa
      121 gcactgaagc ggagagatcg aggtattgat tggcattcag ggtcgaaaaa acgggcgcgc
      181 taaagacggt gggcatgccg acaacgtagt aacgtgcgcc ggagctacca ctgtatggca
      241 tggtcaattt gaaggaggtc gcgccataac tgcacgagag atcgtgcagg ataaagccat
      301 tcgaccagtg aagcaggtcg agcgattggc gcagacgtgg atagctgtac accgaacccg
      361 tcagcgcgcc ggtatctgct tcgacgcatg tcagcgtgta caggtagcca tcatacgata
      421 ggcgtgggga gaaacgaccg atggctgtgc tggtagaagg agcgataacc gcgctgctgc
      481 tccaggttcc tccggagggg ttgtaaatgc aggaaaacag gctgtagcta tcggaataca
      541 cgattgtgta ttgactatta acccaggcaa cggccacacc cgcgccatac gcaatcgtcg
      601 gcaacgacca ggtattcagg gcagaccacg acgaaccgtt atagaaagag cagcccatcg
      661 cttcgccgcc cgaaacgtcg tagagaaaaa agacatcgtt gttgcccccg ctgccaatgc
      721 ccttcgtcaa agcgccgccg ggcggcgata gcacaccgac cggcccgctc catgtcacgc
      781 cattattgct cgatgtccag gcccagagcg tgttgccgcc cgtgccgcgc tgcgcaaagg
      841 cacgaagcgt tccggcgtta tttgagatgg cgcaccctcc atcctgaaac atcgatcccg
      901 atgatcccgg caggttggtc cacgcgctcc actgcgatgc ctgcgatggg tcggtaatac
      961 gctgcacctg gaaggcattg gcgaaacctg agccgcctcg cggcaactgc acacgcacga
     1021 tgctgtaatc attagcaata cacgcatcgt gccacgcatc agctgaccca ggcgtctgat
     1081 attgggcata atgtgcgacg cgatcttcag cgaccaggcg tatcaccgga acgcgcgttg
     1141 cgccgttgag cgcgttcgta agggcggaat caagcgagcg aaccataatg gggcgttata
     1201 cctttctgcg aggatgggca gaggagagaa tcttaggctc gcccaggaga agcaagcgag
     1261 cagcttatag gtgaagtata cagcatgaaa ccaggaaatg caaggagatt ttcatgcctg
     1321 gtacatcctt taaaatccgg caatagccat attttcctat agaggaccgc gcttctggtg
     1381 acgaaatctt aaccagggta ggcaatttga gatcaagcca ggctgtttgt tgttcgcttg
     1441 tctggcctca tatgtctctg gacatgcggc caggttagta cggtatatcg gagaaaggat
     1501 cgtctatgtt gagtttagcg agagtagcgc gtttccctgt aaaacgtctt gtgttgtcga
     1561 gcgccttttt actactggca tttgcatcga tgtttgccaa tacgcaggtg caggcgcgtg
     1621 ggaagacttc cccgccagga aaggagatca acccggcaac agtgatgggg gtgcaggtga
     1681 acggcccctc gtcgctgcca ggcatctcat gggtgcgact agggtatgca acctgcggtg
     1741 cgggcgaccc atcgggggct gcattgcaag gtatcattga cggatatcat cgtcacggtg
     1801 tccacgtgct attgaccgtg tgccaaccca aaccggctca cctgctcgat actgggtatc
     1861 tgaacgatgc ggcgcaggcc aaccccgatg cggtgcagtg cggcaatgaa gagatgaagg
     1921 gcggcgcata caacatttac atttcaccgc aactattcgc cgcgttctac gacctgtgcc
     1981 agaaggccgt tcacgccgtc aacccctcgg cggtgattgt gctggggtcc ctcgacccgc
     2041 atgttgcccg ctacgatacc cagcttttgt tgggcgaggt caactacctg aacgcgatgc
     2101 aggaagccat gaacgcgcgc gtgtataagc atggacactg gacgtggcgg tcacagatcg
     2161 tgggactgat caatagctgg catgacggct atccctcgct ctacatcaac aacctgcgcg
     2221 acctttatac cttctgggcc tgggccttcc acgttgacat gaatcaattg gggaatcacc
     2281 tgtgggtcgt tgaggatacc ggttgtttca aaggctgcgg cgtacctgac aatcagcgcc
     2341 agatcgccat tgcgcacatt ctcgcgctga tcgtcgatgt gcgggtcacg atggaatacc
     2401 gcgttccctt cttcttcttc agcgccagag atttcttcgc cagcggcgcc tactggccaa
     2461 tcggcatcct gaacaataag ggtcaaccca agcccttacg ccaggattta ccaatgggca
     2521 gtcgctccct tctcatgagc tgcggtaaat tgaagacaag ggtcatcaca caggaacagc
     2581 tgcttgcaac catgtactat ggatgccagt taccggaaaa ttcctacaac acgctgattt
     2641 actaaaattg cgccgatagg cagcaggagc gggaagtatt ccctttgcgg gatacttccc
     2701 gctccaactt tagagttttt gctgcttgct tgtggccgac agtatactga aagaggtagg
     2761 cacatcacgt gggaggagag aacaggtggc agaggtggaa cagcacaggc gtcttcaaat
     2821 agcctatttg acggctgagg atccccaaag taagtatgcc tggtctggga ccacctatta
     2881 catggcccag gcgttgcaaa aacattgcgg agatgtgcat tatcttggcc cgattatgtc
     2941 tttcgagcgc cgtgtcatcg gccgactcat gcacgaagct tcgaggaaag cgctggggaa
     3001 aaatatcgca tacgaccgcc ttccctttgt ggcgaaaaag caggcgaaaa ttgcatccca
     3061 acggctcgca tctggtgcct ttaatctcat attcgcgcct atcggcccac cagaagtggc
     3121 cttgctcgaa accgacattc ctattatcct ggccctggat attacctttg ccctacagca
     3181 taattatcat gaacggtact cagcgttact cccgtggtcg gtgaagatga gcaataagat
     3241 cgaagagatg gcctaccagc gagcaagcgc gctaatttat cctgggcgat ggtctgcccg
     3301 atcagccatc gaagattatc acgttgattc tcaaaaagtc cacatcgtcc cattcggggc
     3361 taacctggat gtgcctccaa atgggaagat agttcggaac aggcgtcgct cacatcggtg
     3421 caggctactg tttatggggc ttggttggga gagaaaagga ggggatattg cttttgaaac
     3481 gctcctaaaa ctggaagaga tggggattga ggccgagttg attgtatgcg ggaacactcc
     3541 tcctgccggt ttatcccatg agcgaatgaa ggtaataccc ttcttgaata aacatgatga
     3601 aaagcagtac gaagagttag agcaacttta cacaacctcc gactttcttc tgctccccac
     3661 caggtgcgac tgcttcccca tcgtcttctg cgaggcaagc gcctttggcc tacccgtcgt
     3721 taccgcgcat actggtggag tatcggatgc ggttcttaat ggtatcaatg gctatgtcct
     3781 gccctacgag gcgagaggag ccgactatgc cagagtcatt gcagaacttt tccgggatga
     3841 aacacgttat gctaaattgg tagagtccag cagagcgatg tttgagaaca aatacaactg
     3901 ggatgtatgg ggtgtcacta tgaataccat tctacagcat atggctgccc ctgtaccagc
     3961 atatattcct taagatttcg agaaggaggg agtcctttga ggattccatc ctttgcttcg
     4021 caaggccgca aacatcgtcg gtaataatgt agacattgtc tggttggcgg ggttctaatt
     4081 tcactttgcg tatatcgatt atgttatcaa acgccacagg ctgataagag gagaacgttt
     4141 tttgtacgct cacaaacgtt actggcgtat gaggcaacgc tcgcgcaatc actcttgcgg
     4201 aatcgggcgc agtttgcaac atcgactcat taaccgcctc gaaggcagag cctggcactt
     4261 ttcccaggct attcagatca aaattattcc agcgggaatc tggcgcgccg gtcagtaccc
     4321 agctagtgac gccctggtta tctgccacga acatgccata cctcttcaat gccgtcagga
     4381 taacctgcac atctcgtggg aaggaagaga tgtcgaaact agccttgagg cgcaacctca
     4441 agcccattgg cggcaagttg ggatcggtgc tggaagaggc atagtgcctc gccggccata
     4501 ggaatgccat ttgcgtcgca ttagcgacga cgcgcagcgc atgattgatc gatcccgccg
     4561 cgatttcgtc atagcgcacc agcccaggca gaataggcag ccctgctgca tcagccgatg
     4621 tccagtataa cggcctgagc gcgtctgaat tgagattcca cgtcgcgccg ctgcctgcag
     4681 tccaacttcc acctggctgc ggaaatgcat cccaaagttc gtagagctga cacgtgctgc
     4741 tattc
//
LOCUS       DASZAK010000525         1558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_510631, whole genome shotgun sequence.
ACCESSION   DASZAK010000525 DASZAK010000000
VERSION     DASZAK010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510631"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VGT44_09220"
     CDS             <1..197
                     /locus_tag="VGT44_09220"
                     /inference="COORDINATES: protein motif:HMM:NF015909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581020.1"
                     /translation="CNTKEAPWHIVPSDHKWYRNLAIAHAITAAMRDYRDEWESDLVA
                     RGQQELELIRKMRGEQKLQQ"
     gene            complement(261..>1558)
                     /locus_tag="VGT44_09225"
     CDS             complement(261..>1558)
                     /locus_tag="VGT44_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581021.1"
                     /translation="SVMHVRIRHFYATLEEMFDPSLRGTPFVVGTGTGRPNEPGRVID
                     VSSNALRLGVSPGMPLRRAYRIAPRIRFLPASYDRYQPVLQRLRERYRAYSRIIETIP
                     MADAFIDLRGCEMQFDSPVTLAERLCAEIAEMRLTAMVGVANGKAIAELAALLSRKDG
                     RQGVLYIPPGREASFVQTLPLSLLLQVRTAGAGIPGALPDYAEHESRESHASRPPELE
                     GKGEQADSVAVAEVVAHLRDFGITTFAQVATLTEDGLRRRLGMLGSWLFSLSKGLDIS
                     LVVPDAPPMSQNARVRFQHLAEAEETCVAIRKLADYLGERLVDQRLKGRAITLMMWPN
                     RYASRETRKLAIADDSEEIAVAATEEAIVGQTTLQQYTDDADRIAYQLLALFSAYHRP
                     GTRYSQVQVRVGDIISGLPTFSGYYPALSRARGRQTRKL"
BASE COUNT          310 a          509 c          466 g          273 t
ORIGIN      
        1 actgcaacac aaaagaagcg ccctggcata tcgtcccctc tgatcacaag tggtaccgca
       61 acctggccat cgcccacgct attaccgccg cgatgcgtga ctaccgcgat gaatgggaaa
      121 gcgacctcgt cgcacgcgga caacaggaat tagagttgat tcgcaagatg cgaggcgaac
      181 agaagctaca acaatgagtc cgcagggcga ccgcgagggt cgccctgcgg actgcatgcg
      241 tactcgcccg gaccgtaatc ctacaatttc ctcgtctgtc tgccgcgcgc gcgcgacagc
      301 gccggatagt agccggagaa ggtaggcagg ccagagatga tatcgccgac gcgcacctgc
      361 acctgggaat agcgcgtacc tgggcggtga tacgctgaaa acagcgccag gagttgataa
      421 gcgatacggt ccgcgtcatc tgtatactgc tgcaacgtcg tctgcccaac gatggcttcc
      481 tctgtggcag caactgcaat ctcctcgcta tcgtctgcta tcgccagctt gcgcgtttcg
      541 cggctggcgt agcggttggg ccacatcatc agcgtgatag cgcggccctt caagcgttga
      601 tcgaccaatc gctcgcccag gtagtcggcc agtttgcgga tcgccacgca ggtctcttcg
      661 gcttcggcga ggtgctggaa gcgcacgcga gcattctggc tcatgggagg cgcgtccggg
      721 acgaccaggc tgatatccaa tcccttcgac agggaaaaca gccagctccc cagcattccc
      781 aggcggcggc gcagtccatc ctcggtaagc gtggctacct gggcaaaggt cgtaatgccg
      841 aagtctcgca ggtgcgccac tacttcagcg accgccaccg aatcggcctg ctcgcctttg
      901 ccctccaact ccggcgggcg tgaagcatga ctttcgcggc tttcatgctc ggcatagtct
      961 ggcagcgcgc ctggaatgcc cgcgcccgcc gttcgcacct gcaggagcag cgacaggggc
     1021 agcgtctgta caaacgatgc ctcgcgaccc ggcgggatgt acagcactcc ctggcggcca
     1081 tccttacggc tcagcagcgc ggccagttcc gcgatcgcct tgccattcgc cacgcccacc
     1141 atcgccgtca gcctcatctc cgcgatctcc gcgcagaggc gttcggccag cgtcaccggc
     1201 gaatcgaact gcatttcgca gccgcgcagg tcgatgaagg catcggccat cgggatcgtt
     1261 tcgatgatgc gcgaataggc gcggtagcgc tcgcgcaacc gctgtagcac cggctgatat
     1321 cgatcatacg acgcaggcag gaatcgtatg cgcggcgcga tgcgataggc gcggcgcagc
     1381 ggcataccgg gactgacccc caggcgcagc gcattcgatg aaacatcgat cacgcgcccc
     1441 ggttcgttgg gccgaccggt cccggtgccg accacgaagg gcgtgcctcg cagcgatgga
     1501 tcaaacatct cctcaagcgt cgcgtaaaaa tgacggatgc gcacgtgcat cacactct
//
LOCUS       DASZAK010000526         4530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_617751, whole genome shotgun sequence.
ACCESSION   DASZAK010000526 DASZAK010000000
VERSION     DASZAK010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617751"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VGT44_09230"
     CDS             <1..714
                     /locus_tag="VGT44_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV2581022.1"
                     /translation="ARGDLGVEMAPEKVPLIQKRIISRCNDLGLPVITATQMLESMIT
                     NPRPTRAEASDVANAILDGTDAVMLSAETAAGAYPIEAAQMMVRIALETEAGNRTARQ
                     PQCKQLTQAHAVSHAARALTEEASVQAIVVFTRSGLSAHLISKDRPRTPILAYTPTEQ
                     VYRQLALWWGVWPHRLSMQGSTEELIAVAEKRLQEDNLVQRGQHVVIMGGMPVASQAR
                     TNFVKLHRVGDASPGQAKE"
     gene            890..2425
                     /gene="guaB"
                     /locus_tag="VGT44_09235"
     CDS             890..2425
                     /gene="guaB"
                     /locus_tag="VGT44_09235"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV2581023.1"
                     /translation="MSSTYAPDPDALFSSREDTFDSKFSQEGLTFDDVLIVPAASSVL
                     PREVSTQTRLTRSITINIPIVSAAMDTVTEARLAIALAREGGLGVIHRNLSIDDQAQE
                     VDKVKRSESGMITDPITLEPTALLSEALAAMKRFHISGIPVTENGKLVGILTNRDIRF
                     ETDTQQPIADLMTRERLITVPVGTTLEGARDSLHRYKIEKLPVVDEQGMLKGLITMKD
                     IQKKILYPNAAKDEFGRLRVGAAVGVGPDVEARCAALIEEGVDVLVVDTSHAHSHMVL
                     DAVARVKQRFGQRAQLMAGNVVTAEATEALIQAGVDAIKVGIGAGAICTTRIIAGVGV
                     PQITAIYDCARVARSYSVPVVGDGGIQYSGDISKAIAAGADTVMLGSLLAGVDESPGE
                     LIISRGERFKDYRGMGSIAAMKQRSYSKDRYFQGDVIEENGLIAEGIEARVPYKGMLG
                     PLVFQLVGGLRQSMGYAGCPTIADMQEKTRFVRITNAGLRESHPHDVMVTKEAPNYGL
                     KNR"
     gene            complement(2492..3919)
                     /locus_tag="VGT44_09240"
     CDS             complement(2492..3919)
                     /locus_tag="VGT44_09240"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEV2581024.1"
                     /translation="MSITHDTASEPGFGTQPAPTTASFPPDFLWGAATSAYQVEGAVH
                     EDGRAPSVWDRFAATPGATYRGETGEVAADHYHHMPEDVALMSDLNLSAYRFSLSWPR
                     ILPQGTGAINDKGLDFYDRLVDTLLARGIQPLATLYHWDLPLALHERGGWLNRDTAYA
                     FADYAEVVTRRLGDRVQWWLTHNEPWCSAYLGFALGIHAPGLHDKQLAVNAGHHILLS
                     HGLAVQRMRAHLAPGARAGIAIDFYPVYAADERPETLRAVKQADTFRNRWFLDPVFRA
                     HYPDDLFTDLGVAPPPVLADDLSIISAPIDFLGVNYYSRMLVQDRKDGTPDHVAAAGS
                     FEAIQHIPGASYTEMGWEIFPDGLANILTRIHHEYSPKAMVVTESGAAFPDQWDGNGT
                     IHDQQRIDYLRAHIQTVARTIRHGVPIKGYVVWSFLDNFEWAEGYRKRFGLVYVDYPT
                     QRRVIKDSGRWFASFVASQREARSS"
     gene            complement(3972..>4530)
                     /locus_tag="VGT44_09245"
     CDS             complement(3972..>4530)
                     /locus_tag="VGT44_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2581025.1"
                     /translation="FLVLPGLPLTIVLASLASANAAANKNEFVIALVLLFTSWSYGAR
                     VLRAQTLSLKEREFVTAARASGETSLRIIFAEILPNELTLVASTFIGTFVYAIGAEVA
                     LEFLGLGDISKASWGVILFWAQNDGALIIGKWWQFVPAGLCVATLCAGLAFINFGIDE
                     LANPRLRLERPPRKRLLPGKQKARA"
BASE COUNT          983 a         1326 c         1386 g          835 t
ORIGIN      
        1 gcacgcggcg acctgggcgt cgagatggca ccggagaaag tgccgttgat ccagaaacgc
       61 atcattagca ggtgtaacga cctggggctg cccgtgatta ccgcgaccca gatgctcgaa
      121 tcgatgataa ccaatccgcg ccccacgcgc gccgaagcca gcgacgtagc caacgccatc
      181 ctcgacggca ccgacgcggt catgctgagc gccgagaccg ctgcgggcgc atatcccatt
      241 gaggccgcgc agatgatggt gcgtatcgcg ctggagaccg aggcgggcaa tcgcacagcc
      301 aggcagccgc agtgcaagca attgacccag gcccacgccg tcagtcacgc cgcccgagcg
      361 ctgaccgagg aggccagtgt gcaggccatc gtcgtattca cgcgctccgg cctctcggcg
      421 cacctcatct cgaaagaccg cccgcgcaca cccatcctgg cctatacgcc caccgagcag
      481 gtctatcgcc aactcgcgct ctggtggggc gtctggccgc atcgcctttc catgcagggc
      541 agcacggaag aactgattgc cgttgccgaa aagcgtttgc aagaagataa cctggtgcag
      601 cgtggccagc acgtcgtgat tatgggcggg atgccggtgg caagccaggc gcgcacgaat
      661 ttcgtgaagc tgcaccgcgt cggagacgcc tcgccaggtc aggccaaaga gtgagctgtc
      721 aatttgacat aggatgtttc aggatgatac cataccagga ataggatgaa acaccgccag
      781 ataccatcaa cgatgtgcga gcaatcatta catcaccccg gtgggaggtc cagcttacga
      841 atgtacgtgc gctggacgtt tttgtaagta aggaggatca accgccatta tgtcttcgac
      901 ctacgcgcca gacccggatg cgctcttttc gtctcgtgag gatacgttcg atagtaagtt
      961 ctcccaggaa ggtctgacct ttgatgatgt cctgatcgtt cccgccgctt cgtccgtgct
     1021 gccccgcgag gtctcgacgc aaacccgcct gacgcgctcg atcaccatca atatcccgat
     1081 cgtcagcgcg gcgatggata ccgtgacgga ggcgcgactc gctatagccc tggcgcgcga
     1141 aggcggcttg ggcgtgatcc accgcaatct ttccatcgat gaccaggcgc aggaagtcga
     1201 caaggtcaaa cgctccgagt cgggcatgat aaccgacccc atcacgctcg aacccactgc
     1261 gctgctgagc gaggcgctag ccgccatgaa gcgcttccac atctccggta tccccgtcac
     1321 cgaaaacggc aaactggtcg gcatcctgac caatcgtgac attcgtttcg agaccgacac
     1381 gcagcaaccc atcgccgacc tgatgacgcg cgaacgcttg attaccgtgc cggtcggcac
     1441 tacgctggaa ggggcgcgcg acagcctgca tcgctacaaa atcgagaaat tgccggtggt
     1501 ggatgaacag gggatgctca aaggtctgat taccatgaag gatatccaga agaaaatcct
     1561 gtatcccaac gcggccaaag atgaattcgg acgcctgcgc gtcggcgctg ccgtgggagt
     1621 cggccccgat gtcgaagcac gctgcgccgc gctgatcgag gaaggggtcg atgtgctggt
     1681 ggtcgatacc tcccatgcgc actcgcacat ggtgctggac gcggtggcgc gggtgaagca
     1741 gcgcttcggc cagcgcgcgc agttgatggc gggcaacgtc gtcacagcag aggcgaccga
     1801 ggcgttgatc caggcgggcg tagacgcgat caaggtgggc atcggcgccg gcgcaatttg
     1861 cacgacgcgc atcatcgcgg gtgttggcgt gccgcagatc accgccatct acgactgcgc
     1921 gcgcgtggct cgcagctaca gtgtgccggt ggtgggagac ggcggcatcc agtattccgg
     1981 cgatatctcg aaggccatcg cggcgggcgc ggataccgtc atgctgggca gcctgctggc
     2041 gggcgtcgat gaaagccccg gcgagctgat catctctcgc ggcgagcgct tcaaggatta
     2101 ccggggcatg ggttcgattg ccgccatgaa gcagcgcagc tatagcaaag accgctactt
     2161 ccagggcgat gtgatcgaag aaaatggcct gatcgccgag ggtatcgagg cgcgcgtccc
     2221 ctacaagggc atgctggggc cgctcgtctt ccagcttgtc ggcggtctgc gccaatcgat
     2281 gggctacgcc ggctgcccca ccatcgccga catgcaggag aaaacgcgct tcgtgcgcat
     2341 caccaacgcg gggttgcgcg agtcgcaccc acacgacgta atggtgacga aagaagcgcc
     2401 caactacgga ttgaagaata gataaaaaaa gcacacgagc aatgatgcgc cgcattccag
     2461 acgcatcatt gctcgtgtgc tttcatcctg attacgacga gcgcgcctca cgttgtgagg
     2521 cgacgaagga ggcgaaccag cgaccgctgt ccttgatgac gcggcgctgg gtgggataat
     2581 cgacgtagac caggccgaag cgtttgcgat agccctcggc ccactcgaag ttatccagaa
     2641 aagaccagac gacgtagcct ttgatgggaa cgccgtgacg gatggttcgc gcgacagttt
     2701 gaatgtgcgc tcgcaggtag tcgatgcgct gctggtcgtg tatggtgccg ttgccatccc
     2761 actgatcggg gaaagcagcg ccgctctcgg tgacaaccat cgctttcggg gagtactcgt
     2821 gatgaatgcg cgtcaagatg ttggcgagac catcagggaa tatctcccag cccatttctg
     2881 tatatgatgc gccaggaatg tgctggatgg cttcaaagct tccggcagca gctacgtggt
     2941 cgggtgtgcc atctttacgg tcctgcacga gcattcgcga atagtagttg acgccgagga
     3001 agtcgatggg ggcggatatg atggataaat cgtccgcaag aacaggcgga ggagctactc
     3061 ccaggtcggt aaaaagatcg tcggggtaat gcgcgcgaaa gaccggatcg agaaaccagc
     3121 gattgcgaaa cgtatctgcc tgtttaaccg cgcgtaacgt ttcggggcgt tcatcggcgg
     3181 cgtagactgg gtagaagtcg atggcgatac ccgcccgcgc accgggcgca agatgcgcgc
     3241 gcattcgctg caccgccagg ccgtgcgaga gtaagatatg gtgaccggcg ttgactgcca
     3301 gttgcttatc atgcagaccg ggtgcatgga tgcccagtgc gaagccgagg taggcgctgc
     3361 accagggttc attatgcgtg agccaccact gtacgcgatc acccaggcgg cgcgtcacca
     3421 cctcggcgta gtcggcgaat gcataagcgg tatcccgatt gagccaaccg ccgcgttcat
     3481 gcaaggccaa tgggagatcc cagtgataga gcgttgcgag tggttgaatg cccctggcga
     3541 gcaaggtatc gaccaggcga tcataaaaat ccaaaccttt atcgtttatc gcgcccgtac
     3601 cctgcggcag gatacgcggc caggataacg agaagcgata ggcgctcagg ttaaggtcgg
     3661 acatcagcgc aacgtcttct ggcatatgat gataatggtc tgccgcgact tcgcctgttt
     3721 caccccggta ggtcgcgcca ggtgtggcgg cgaaacggtc ccacaccgat ggcgcgcgcc
     3781 cgtcttcatg caccgcgcct tcgacctggt atgccgaggt tgcggccccc cagagaaaat
     3841 ccggtggaaa ggatgcagtt gtcggagcgg gctgcgtgcc gaatcctggt tctgacgcgg
     3901 tgtcgtgtgt aatgctcatc gagtctcctc tacaaggcgc ctgttacggc gcttgccgat
     3961 acaacgttgt attacgctct tgccttttgt ttgcccggca acaacctctt gcgtggcgga
     4021 cgttcgagac gcaggcgcgg gttcgccagt tcatcgatgc cgaagttgat aaacgccagg
     4081 cccgcgcaga gtgttgccac acataacccc gccggaacga actgccacca ctttccaatt
     4141 atcagcgccc cgtcattctg cgcccagaac aatatcactc cccagcttgc cttggatatg
     4201 tcgccaagcc cgagaaattc cagagccacc tctgcgccga tcgcatagac aaaggtgccg
     4261 ataaaggttg aagcaaccag cgttaattcg ttgggcagga tttcggcaaa gatgatgcgt
     4321 agagacgtct caccggatgc gcgtgcggct gtgacgaatt cgcgctcctt caacgaaagc
     4381 gtctgcgcgc gtaagacgcg cgctccatag ctccaggacg taaagagcaa gaccagcgcg
     4441 atcacaaact catttttatt cgcagcagcg ttggcactgg ctaatgaagc gagcacaatg
     4501 gtaagaggaa gacccggcaa gaccaggaaa
//
LOCUS       DASZAK010000527         2386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_510697, whole genome shotgun sequence.
ACCESSION   DASZAK010000527 DASZAK010000000
VERSION     DASZAK010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510697"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..304)
                     /locus_tag="VGT44_09250"
     CDS             complement(62..304)
                     /locus_tag="VGT44_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEV2581026.1"
                     /translation="MTDTELLHHITELVNEEHSLLQQSEGVGLDEDQHERMKQLEVSL
                     DQCWDLLRQRRARREFHLNPDEAQVRPETTVEHYQQ"
     gene            complement(390..2255)
                     /locus_tag="VGT44_09255"
     CDS             complement(390..2255)
                     /locus_tag="VGT44_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581027.1"
                     /translation="MITFIDKEVRLQAIERQLARLRRRLNALESRSDRFGWSRVAIFF
                     GGVLLSVLAYFRFGWWLSLAGTLAMLAVYSVVAYLHRRITRSMTRYRILAHIKSTQLA
                     RMRLDWEHIPAVQSDTIGLEHPFEIDLDISGKRSLHQLLNTAVSQDGSQRLRDWLLST
                     NPNVETIAYRQALIRELAPLTIFRDKLLMKTLLATHNQGSQWNGKRLLLWLDEHQTSY
                     RFLPFILLALAFSLTTITLFVLNLTINIPQYWVIALLLAIVYLFLTKDRRGDLFDDAH
                     DLGDAFSQLGIIFDYLETYRYGKNQQLKALCQPFLGQPELRPSTFAKNIARIASAATL
                     QKNIMLWIIVNVLMPWDFYLAYRFQYYKAQIAALLPCWLDAWSELEALNSLANFAYLN
                     PEYTLPEVIASADITTPGDQPIFEAKGLGHPLIPREQRVVNDFSMIAPGEIDIITGSN
                     MSGKSTFLRTLGINLCLAYAGGPVNASQLRTSQFRLFSCIRISDSVTDGYSYFYAEVK
                     RLKALLNDLDVTSAIPLFFLIDEIFKGTNNRERLIGSEAYIAALVGRNCVGVVSTHDL
                     ELVKLADRLPHIKNYHFREDVLDGHMVFDYVLHEGPCPTTNALKIMRLEGLPIDL"
BASE COUNT          542 a          621 c          660 g          563 t
ORIGIN      
        1 agcgttatcg tagtggcgat cccttgcggt cgccatgccc tcgcgctcgc cacgccaact
       61 actattgctg gtaatgctcc acagtcgtct ccggtcgaac ctgagcctca tccgggttca
      121 gatggaattc gcggcgcgct cgacgctggc gcagcaaatc ccagcattga tccaggctca
      181 cctcgagctg cttcatacgc tcgtgctggt cttcgtccag cccaacacct tccgattgtt
      241 gcagcaacga atgttcttcg ttgacaagct cggtgatatg gtggaggagt tctgtatcgg
      301 tcatatggtg ctccttgtca tcctgaacat tgagaatatg cttcctctgc tattctaaac
      361 aaattgtgcc gaatgcgcaa ttccctctat tataggtcta tcggaagccc ctccaggcgc
      421 ataatcttga gcgcattggt tgttggacat ggaccctcgt gcagtacata atcaaagacc
      481 atatgcccat ccaggacgtc ctcccggaaa tggtagttct ttatgtgtgg caatctgtct
      541 gccagcttga ccagttctaa atcgtgtgtc gagacaactc ccacacagtt ccggcctacc
      601 agcgcagcaa tgtacgcttc gctgccaatc aaccgttcgc ggttgttggt ccctttgaaa
      661 atctcatcga tcaggaagaa gagcggtata gcgctggtta catccagatc gttcaacagg
      721 gccttgaggc gtttcacctc ggcgtagaag taggaatagc catcggtcac ggagtcgctg
      781 atgcgaatgc aggaaaagag ccggaactgg gaggtgcgca gctggctggc attcaccggt
      841 ccaccggcat aggccaggca gaggttaata ccgagcgtgc gtagaaacgt gcttttgccg
      901 gacatgtttg agccggtgat gatatcgatc tcgcccggcg cgatcatgga aaaatcgttg
      961 accacccgtt gctcccttgg aatgagtgga tggccgagac ccttcgcttc aaagatcggt
     1021 tgatcgccgg gagtagtaat gtccgcgctc gcgataactt ctggcagggt gtattcggga
     1081 ttgaggtaag caaaattcgc cagcgagttc aatgcctcaa gctcagacca tgcatccagc
     1141 cagcatggta ggagtgccgc tatttgcgct ttataatact ggaaacgata ggccaggtag
     1201 aaatcccagg gcatcagtac attgacgatg atccagagca tgatgttctt ctgtaacgtc
     1261 gcggctgagg cgatgcgagc gatgtttttc gcgaacgtcg aggggcgcag ctccggctgg
     1321 cccaaaaatg gttggcataa cgctttgagc tgctggtttt tgccgtagcg ataggtttcc
     1381 agatagtcga agatgatacc gagctgtgag aaggcgtctc ctagatcatg cgcatcgtca
     1441 aaaaggtcgc cgcgccggtc ttttgtaagg aaaagataga cgatggcgag cagcagcgcg
     1501 atcacccaat actggggaat atttatcgtg aggttcagta caaacaaggt gatggttgta
     1561 agagagaagg ccagagcgag taagataaac ggcagaaacc ggtaggacgt ttgatgttcg
     1621 tcgagccaga gcagcagcct tttcccattc cattgcgacc cctggttatg cgtggcaagc
     1681 agggttttca tcagcagctt atcccggaag atcgtcagtg gtgccagttc gcgtatcaac
     1741 gcctgcctat aagcgatcgt ctcgacgttg gggttggtag ataagagcca atcacgtaaa
     1801 cgctggctgc catcctggga gacagccgtg ttgagcaatt gatgcagcga gcgttttccc
     1861 gaaatatcga ggtcgatttc aaaaggatgc tccaaaccta tcgtatccga ttgcactgcc
     1921 gggatgtgtt cccagtcaag ccgcattcgc gcgagttgcg tagatttgat gtgcgccaga
     1981 attctgtaac gggtcatgct gcgagtaatg cgccggtgta aatacgcgac aacgctatag
     2041 accgccagca tcgccagtgt tccagccagc gacagccacc agccgaaccg gaagtaagcc
     2101 aggacactga gaaggacgcc cccgaagaag atagcgacgc gcgaccagcc aaagcggtcg
     2161 ctccggcttt ccagggcatt caagcgccgc cgcaggcgag caagctgtcg ttcgattgcc
     2221 tgtagtcgca cctctttatc gatgaatgta atcaactgtt ttgcttgttt tctataatgt
     2281 tggaggcgag ccagggcgat cctcgcgatc tccctgctcg cttacgccgg tttgctcttg
     2341 ttggccagtt cctcaaggta cgtctgcata accgcctggt gttccg
//
LOCUS       DASZAK010000528         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_119092, whole genome shotgun sequence.
ACCESSION   DASZAK010000528 DASZAK010000000
VERSION     DASZAK010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119092"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1936)
                     /locus_tag="VGT44_09260"
     CDS             complement(<1..1936)
                     /locus_tag="VGT44_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581028.1"
                     /translation="MARDLQQVTQGTPSSIRRTGPRDPGGRSYRRGGGSPVARAVLIG
                     LGIALALVVLEGLAWVYDPLNIFGSTHGVSRTLTDLFALLAHTPLLLAIPLLEFAAAV
                     VLALLLTRPLVLSRYCKAAIQAGERYRVAHTSLPNWPSLYETTVSYYQYTPDPAVPVV
                     TRERSVLDLARGSPFSTEPRSHLVLFGEAGSGKTSALYCCQFYALQERRRLLYGREKV
                     PVYVPLRQYALYLRARASENEQDVASPPVEPDAARLLDFLYYSDLPGMHHLRPYLHRL
                     AAQGRLLLLCDGLHDVEESEQPGVIAELADLMSQERNRVLLTCRAIDARRQPSLAEAI
                     EANLVPRAFLQPLTATQMRAFVEQYIESDSAGKKWKHTAGQVMEIITRTRLQICCETP
                     LMLLSLLSVVDGMGMERGKRLDTRGRLLRAYVGQRITQTQRLPSWSGRAPTEKDILLF
                     LGELACAARWSNVASAVQLHEKPHLFGEWLPSLEHHAGALLAWLEARAGEMVLSDTLH
                     AAYSQEEIAHLLQFALNASLIEISPHGFVSFRYELIASYAIAEYFAALQVAADTTLQK
                     TRFAGLLAQALKREDSSDAFTRWSVPVALWAGLVDEPATYAARFVEYAQDHPDAAQES
                     LSLSLLSLGVAAYPPRLSAAP"
BASE COUNT          404 a          600 c          642 g          354 t
ORIGIN      
        1 ccggcgcagc cgatagacgc ggaggatagg cggcgacccc aagactgagc aggctgaggg
       61 ataggctctc ttgagcagca tcgggatgat cctgagcgta ttctacgaag cgagcggcgt
      121 aggtggccgg ttcgtcgacg agacccgccc acagtgcgac gggtacgctc cagcgtgtaa
      181 aggcatcgga gctatcctcg cgtttcagcg cctgggcgag caatccggcg aagcgcgtct
      241 tttgcagcgt ggtatcagca gcaacctgca aggcagcgaa atattcggcg atggcgtagg
      301 aagcgatcaa ttcataccgg aagctgacga agccgtgcgg gctgatctcg atcagcgagg
      361 cattcagcgc gaattgcagc aggtgagcaa tctcctcctg gctgtaggca gcgtgcaggg
      421 tatccgagag caccatctcc cccgccctgg cctccaacca ggcgagcagc gcacccgcgt
      481 gatgctccag cgatggcagc cattcgccga acaggtgtgg cttttcgtgc aactgaacgg
      541 cggaggcgac attcgaccag cgcgcggcgc aggcaagttc gcccaggaac agcaggatgt
      601 ctttttcggt gggagcgcgt ccgctccagg atggcagtcg ctgcgtttgc gtgatgcgct
      661 ggccaacgta ggctcgcagc aggcgaccgc gcgtatcgag gcgtttgccg cgctccatac
      721 ccatgccatc gacgacgctt aagagggaaa gtagcatcaa gggcgtctcg cagcagattt
      781 gcaggcgtgt gcgcgtgatg atttccatga cctgcccggc ggtgtgcttc cactttttgc
      841 ccgcgctatc gctttcgatg tattgctcga cgaaggcgcg catttgcgtc gctgtcaacg
      901 gctgaaggaa ggcgcgcggg acaaggttcg cctcgattgc ttccgcgagg gatggctggc
      961 ggcgcgcatc gattgcccgg caggtgagca ggacacggtt gcgctcctgg ctcatcaggt
     1021 ccgccagttc agcgataacg ccgggctgct cgctttcctc gacatcgtgc agcccatcac
     1081 agagcagcag caggcgaccc tgggcggcaa gccggtgaag ataaggccgg agatggtgca
     1141 ttccgggcag gtcgctgtaa tacaggaaat ccagcagccg ggcggcgtcc ggctcgaccg
     1201 gcggggaggc gacatcctgc tcattttccg aggcgcgcgc gcgcagatag agcgcgtact
     1261 ggcgcagagg cacgtacact ggcacttttt cgcgcccata gagcagacgc ctgcgttctt
     1321 gcaaggcata gaactggcag caatacagcg cagaggtttt gccgcttcca gcctcgccaa
     1381 acagcaccag gtgcgaacgt ggctccgttg aaaacgggct gccgcgcgcg agatcgagca
     1441 ccgaacgttc gcgcgtcacc accggcacag caggatcggg tgtatactgg tagtagctca
     1501 ccgtcgtttc atagagcgat ggccagttcg gcagtgaagt atgtgcaaca cggtagcgtt
     1561 cacccgcctg tatagccgcc ttgcaatagc ggctgaggac gagcgggcgc gtcaagagca
     1621 gcgccaggac aacggcggcg gcaaattcca acagggggat cgccagcaac agcggcgtgt
     1681 gcgcaagcag tgcgaacagg tcagtgagcg tgcgcgatac gccatgcgtc gaaccgaaga
     1741 tgttcaacgg gtcgtatacc caggcgagac cttcaagcac caccagcgct agcgcgatgc
     1801 ccaggccgat caggacagcc cgcgcgaccg gcgaaccgcc gcctcgcctg tacgagcggc
     1861 cccccggatc acgcggccct gttcgtctta tcgatgaggg tgttccctgc gttacctgct
     1921 gcaaatctct tgccatacct caaacaccgg tccttcaaca tatgtagttc agcgtaggaa
     1981 cagtatagcc tacctgtctc
//
LOCUS       DASZAK010000529         3781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_581668, whole genome shotgun sequence.
ACCESSION   DASZAK010000529 DASZAK010000000
VERSION     DASZAK010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3781
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581668"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VGT44_09265"
     CDS             <1..523
                     /locus_tag="VGT44_09265"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2581029.1"
                     /translation="LYAELERRGAVLYIHPAGVAACSPLIADYGLTWPIGAPIEDTIA
                     ATHLIFKSIPVRYPHIKIINSHLGGALPMLLQRLDNQYRWAAPQMTEPPSAQARRMWY
                     DTVGHFHVPALRCACESFGADRLVLGTDFPYLAGDLYRRSVFYIQNAGLSTSDEHRIL
                     EVNAASLLQLERK"
     gene            724..1068
                     /locus_tag="VGT44_09270"
     CDS             724..1068
                     /locus_tag="VGT44_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581030.1"
                     /translation="MLPSKWLFLPIWVMYLLALVAFEVVADVLAKQFAINGKIVFGVL
                     SLGGYILANIAWLISLRSGGTLSQGSVIFSALNGVVAVVLGLFVFHEKASPYQLIGMM
                     LGIAAIVFLSLN"
     gene            complement(1117..2328)
                     /locus_tag="VGT44_09275"
     CDS             complement(1117..2328)
                     /locus_tag="VGT44_09275"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV2581031.1"
                     /translation="MAQFDSVYIPYGGYWSTPFVRWQGNFSHLHPIRFAADVAQRAIR
                     ERDIALESFDTLFLGMTVIAEHSFYGAPWLAGLIGAAGISGPTINQACATSARVLASA
                     AYEVESDAGRSILAITCDKTSNGPHIYYPNPLAPGGKGESEEWVWDNFNCDPYAQNAM
                     LETAENVAAESGISWEEQNAVALLRYQQYQDALQGDAAFHRRYMVTPLDVKDLSGRKV
                     LATVTDDEGVYPTTAAGLASLRPVKQGGTVSFGTQTHPADGNSGVVMTSRERARELSR
                     DRAIEIQVLGFGQARAKKGFMAQATVPAARSALKAAGVSLSNIKALKTHNPFAVNDIY
                     LARELGIPLESFNNYGCSLIFGHPQGPTGMRLVIELIEELALLGGGYGLFTGCAAGDT
                     GAAIVLRVDVQ"
     gene            complement(2343..>3781)
                     /locus_tag="VGT44_09280"
     CDS             complement(2343..>3781)
                     /locus_tag="VGT44_09280"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2581032.1"
                     /translation="TSWRSRNWNSAWLPCGHYAKADPTIAVKEEKYMTYPRYFNARVE
                     TMPIKQIQELQERKLRKQLAYNYQNSVFYRQKFDEVGARPEDIRAIEDLAKLPFTTKE
                     ELRDSQLEHPPLGRHAAVPMSRVVRIHSSSGTTGRPSYVGITRHDRDTWIESVARVYW
                     SEGVRPDSVVVMGFGMSFFVGGLPLHNSIEEIGATFIPVGTGASDRLVTSIQNLGATQ
                     LTCTPSYAIYLAEYVRNKFHVEPSTLGIRRIQVGAEPGGGVPAVRQRIEEDWGAFVTE
                     GIGNADVIPDYAADCDERNGMHFLAPDFAILEIVDPETGQSLPLDRPEVQGEMVFTHI
                     DRECVPLVRFRTRDRVVLWTDPCVCGRTGMRLRCIGRTDDLLILRGVNVWPSAIKDVL
                     MAMRPRTTGEVQIVLSQPGPRVDPPLHLKVEYGSDVEDLAALKRDIEDVMRDKLIFTA
                     NVELLPPGTLPRYEMKSQLIRKAYEQVR"
BASE COUNT          776 a         1109 c         1056 g          840 t
ORIGIN      
        1 actctacgca gaactcgagc ggcgaggagc cgtgctctac atccatcccg caggcgtagc
       61 cgcctgctcg ccactgatcg cggactacgg gctgacctgg cccatcggtg ctcccattga
      121 ggacacgatc gccgccacgc acctcatttt caagagcatt ccagtgcgtt acccccacat
      181 caagatcatc aactcgcacc tgggaggagc gctgccgatg ctcctgcaac gcctggataa
      241 tcaataccgc tgggcggccc cgcagatgac cgaacctccc agcgcgcagg ccaggcgtat
      301 gtggtacgat acagtcgggc actttcacgt cccggcgcta cgctgcgcct gtgagtcatt
      361 tggagctgat cgtctcgtgc ttggaaccga ctttccttat ctcgcgggcg atctataccg
      421 gcgctcggta ttctatatcc aaaatgccgg gctatccacg tctgatgagc accggattct
      481 cgaagtcaac gcagcatcgc tgttgcaact ggagaggaaa taagcaggag tcccatcatg
      541 gacgaacata attcaacgac gcctcctctg atatatttga tttcctccaa tcgctgagtt
      601 ttcccttttt tgggtaagac agcagaacgg catcagcccc gcgggctaga tgccagtaat
      661 gctttgttgt cttgcccgcc cctcatattc tcccccacag atcgataaag aaaggatcat
      721 cgtatgttgc cttcaaaatg gctgtttctt cccatctggg tgatgtacct gttagccctc
      781 gtggcttttg aagtggtggc tgatgtgctc gccaaacaat tcgcaattaa tggcaagatt
      841 gtgtttggtg tactgtctct tggcggctac atcttagcca acatcgcgtg gttgattagc
      901 ctgcgcagcg gcgggacgct gagccagggt tcggtgatat tctccgcgct gaatggagtg
      961 gttgcggtcg tgttggggct tttcgttttt cacgagaagg ccagtcccta ccagcttatc
     1021 ggcatgatgc taggtatcgc agcaattgtg ttcttgtcgt tgaattaacc cgatactatt
     1081 cttcatggag catgaccggc ttcgcaaccc tgacctttac tgcacgtcca cgcgcaagac
     1141 gatggccgca cctgtatcgc cagcagcgca gccggtgaag agaccgtagc cgcctccaag
     1201 caaggcgagt tcttcgatta actcaatcac caggcgcatg ccggttggtc cctggggatg
     1261 gccgaaaatg agcgagcagc cgtagttgtt gaagctttca agagggatac ctaactcgcg
     1321 cgccaggtag atatcgttga cagcgaaggg gttatgcgtt ttcagcgctt tgatattgga
     1381 cagactgact cccgccgctt tgagcgcgct gcgcgccgct ggaacggtcg cctgcgccat
     1441 gaagcccttc ttagcgcgcg cctggccaaa ccccaatacc tgtatttcaa tggcccggtc
     1501 acgactgagt tccctggcgc gctcgcgcga ggtcatcact acaccgctgt tcccatcggc
     1561 aggatgcgtc tgcgtaccga agctgacggt gccaccctgt ttcacagggc gcaaacttgc
     1621 cagccctgcc gcagtggtgg gatagacacc ttcatcatcc gtcaccgttg ccagcacttt
     1681 gcgcccggaa agatccttca catcaagggg tgtcaccatg tagcggcgat gaaaggcagc
     1741 gtcaccctgt agcgcatcct ggtactgctg gtagcgcaat agtgcgacag cgttttgctc
     1801 ttcccaggag atgcctgatt ccgccgcgac gttttcggcc gtctcgagca ttgcattctg
     1861 ggcatagggg tcgcagttga agttgtccca cacccactcc tcgctctcgc ccttgccacc
     1921 gggagcgagc ggatttgggt aatagatatg cggcccattg ctcgttttgt cgcaggtgat
     1981 cgccaggata ctgcgcccgg catcagactc aacctcgtag gcggcgcttg ccagcacccg
     2041 cgcggaggtt gcacacgcct ggttgatggt cgggccggaa attccggcag ctccaataag
     2101 tccggccaac cagggcgcac cataaaagct atgctcggca atcaccgtca tgccgaggaa
     2161 tagcgtgtcg aagctctcta gagcgatatc gcgctcgcgt atggcacgtt gagcaacatc
     2221 agcagcgaag cggatcgggt gcaagtgcga aaaattgccc tgccatcgca caaatggggt
     2281 agaccagtac ccaccataag gaatatagac cgagtcgaat tgtgccataa ctgacctccc
     2341 tgttatcgta cctgttcata cgccttgcgg atcaactgcg atttcatctc gtagcgtggc
     2401 agcgtccctg gtggcagcag ttcgacattt gctgtaaaga tgagcttgtc acgcatcaca
     2461 tcttcgatat cgcgtttcag cgccgccagg tcttcaacat ctgacccata ttctaccttc
     2521 agatgtaatg gtgggtcaac acgcgggccg ggctgcgaaa gcacaatctg cacctcgcct
     2581 gtcgtgcggg ggcgcattgc catcagcacg tccttgatgg cagatggcca tacgtttaca
     2641 ccgcgtaaga taagcagatc gtcggtacga ccgatgcagc gcaggcgcat acccgtgcga
     2701 ccacagacgc aggggtcagt ccagagcacc acgcggtcgc gcgtgcggaa acgcacgagc
     2761 gggacgcact cacgatcaat atgtgtgaag accatttcgc cctgcacttc cgggcggtcg
     2821 agcggcaagg attgacccgt ctccgggtcg acgatttcga gtatggcgaa gtcgggagcc
     2881 agaaaatgca taccgttgcg ctcatcgcaa tcggctgcat agtcggggat cacgtcggcg
     2941 ttgccgatgc cttcggtcac gaacgctccc caatcctctt cgatgcgctg gcgaacggca
     3001 ggcacaccgc cacctggttc cgcgccaacc tggattcgcc ttatgccgag cgtactcggc
     3061 tcgacatgaa atttattgcg cacatactcc gccaggtaga tggcatacga gggggtgcaa
     3121 gtcagctggg tcgcccccag gttctggata gaggtaacca gacggtcgga tgcgcctgtc
     3181 ccaacgggga taaaagtcgc gccgatctcc tcgatggagt tgtgcagggg cagaccgccg
     3241 acgaagaagc tcataccgaa tcccataacc accaccgagt cggggcgcac cccctctgac
     3301 caatagacgc gtgccacgga ctcgatccag gtatcgcggt cgtgcctggt gatgccaacg
     3361 tagctggggc gacccgttgt gccggacgag gagtggatgc gaacgacccg cgacatcggg
     3421 acggcggcat gtcgcccaag gggaggatgt tctagctggc tgtcacgcag ctcctcctta
     3481 gttgtaaatg ggagttttgc caggtcctcg atcgcgcgga tatcttctgg gcgcgctccc
     3541 acctcgtcga atttctggcg ataaaagaca gagttctgat agttgtatgc cagttgcttg
     3601 cgcagcttcc gttcctgcag ctcctggatc tgcttgatcg gcatagtctc gacgcgggcg
     3661 ttaaagtagc gcggataggt catgtatttc tcctctttca cagcaattgt tggatcagct
     3721 ttcgcataat gaccgcaagg cagccacgcg ctgttccagt ttctggatct ccaactggta
     3781 g
//
LOCUS       DASZAK010000530         5475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_189469, whole genome shotgun sequence.
ACCESSION   DASZAK010000530 DASZAK010000000
VERSION     DASZAK010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5475
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189469"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1909
                     /locus_tag="VGT44_09285"
     CDS             <1..1909
                     /locus_tag="VGT44_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581033.1"
                     /translation="AEQAYLNSGVLIMRITAPSLCWMRAQLVTEQAEREALEASAYNL
                     VYELLSGTVLEEKPLDRVIGMTTLMLLPARWKIEKGNFEGALENIAISRSLSFALSKV
                     IQDEMGGTVEELDKEIGDPDTPPEKQKQLKGLRDFYLPLVRTGDYWTFTCDLLTYTIN
                     AKLAESQSKFQEAYRLYDEAARLEEDMRKIILVWMSLYANISTLDGIVGLDAPQHSIR
                     STYFQGMASLSRGDSELMKGDYAEARKHYQNANTTFDTAARSWTLQMERLNSPSQIDG
                     RANADRERRLCLDRMRYCDAKDEMAQAQRYSAYDEFADASEHFDNAAIILNALWKDSL
                     DRETPRNLDLFKASEMFCKGCALLEEARRVSREDIMLKAKEPIDRAVSLFEDASEPLW
                     SKYINALRLEYEADIYKKLSNASASQKNQLQAAVYAQFAQKQASEASDIFKQLGVEKR
                     VSGMDRLAHSMAHSVVESQALSLFSPPNPADAPMASSNNAVQSMKDQLRFGGIQPGQI
                     ISENRLETFLAEMSAIDSNLDDLKQQFGDGDLDQARYLKMEAKRIQQRSAILQKVRKE
                     LEGRDKDLDTVMQDATTGGQEERVLMDRLAEVAQRRGLTSSLIDSLTRHQGTFLPSLI
                     EVGKQLIKLSD"
     gene            2051..3625
                     /locus_tag="VGT44_09290"
     CDS             2051..3625
                     /locus_tag="VGT44_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2581034.1"
                     /translation="MVENSSVPNGPDAYRSYIGATLGEYRLDQVLEESEVGPIFLART
                     SQASFRVQLLTVPPDLASQDRMVWLGRFQREANLVASLRHPGLLPLLDYGIYQSFPYL
                     VMPRLKMTSLSAYLSKQGALDTLLASRYLDQIADALEYAHAQAALHLSLSTDCIFVKE
                     DNSLVVANLGIVRMVETGGTTASLAAQEQERNHFILRDRQGRALYGLGPGSSPAPEQL
                     LVNPVDTYTDVYALGAVLYRMLTGHRVFRGTNAAELAQQHLTAPVPPLSKWRAGIAPQ
                     VDALIAKAMSKEPSQRFRQPGLVANAYHDSVAPNDTQRRAFAVGAPSLVSTQASRDAA
                     MAAAPLAPKQVGPRPSSARPSGTPRGTAGRANNAGISRRRLLVGGGAAVAIVAVTVFA
                     SHYLIGTTTPGGTTTTNGGGPGSTGGSGGHAGTVIAHTSDVQVNSAKTFPIANQQNPG
                     VLVHLQNNSFVAFDSTCTHAGCAVAYNPQDHLLECPCHGASFDPAKGASVVQGPATTP
                     LTSIPIKVNADNTITT"
     gene            3662..>5475
                     /locus_tag="VGT44_09295"
     CDS             3662..>5475
                     /locus_tag="VGT44_09295"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2581035.1"
                     /translation="MPEQYVIVGNGIAGMTAAEILRDENDAADITVIADDPFPVYYRP
                     ALKDYLAGRVREEKLWARPNSFYQDRRIRFVAERVERIQVGQHVAQLANGRQVGYSRL
                     LLASGARASRLSCPGINLAGVTTLRTVADYQEVQQQLNQARHIVVSGSGTLALETIET
                     LRHRGFGVTHLLRKRTLWSEVRDATASDLVLQQERRDGVDVHVEEEIAEITGKDGRVT
                     GITTSSGARIPCEIVIIAIGVDPIVDFVKSSGIPCGRGMKVDAAMRSNAPDIFAAGDI
                     LETTDASTGRTRVIGQWFPAIQQARAAAYSMLDMLDTRRPFQSSNFYNATFLYGLEFA
                     SVGLTQAPKGGQGYQELVADPLPRTYRKALLKNGVPVGMLMLGNRKDGLALKRAIDHG
                     VNLTPVASQLFVEGFKLDAWLDKQGVPPPILSANKEGYVAKEAPVSDGATVVAAGVDS
                     AAVPPAPRREASLVPVDVGAGGEVKLSEEQAMTVGRQAGVFLLIDHASVSRHHAEITY
                     VNGQYILRDLGSSNGTFVNETRLEMGSTRALEAGQRIRFGKVSYQVQMRSSQAEQAAP
                     RTVSAETSYIRELTTGFFDPMAAGAIPATSYQPVLNADG"
BASE COUNT         1204 a         1471 c         1656 g         1144 t
ORIGIN      
        1 ggcggagcag gcttacctga attccggcgt attgataatg cgtataacgg ccccgtcgtt
       61 atgctggatg cgcgcgcaat tggtgacaga acaggccgag cgcgaggcat tagaagcaag
      121 tgcctacaac cttgtctatg agcttttaag tggaactgtg ctggaggaga agccgcttga
      181 tcgggtaata ggtatgacaa cacttatgct tcttccggca agatggaaga ttgaaaaagg
      241 gaactttgag ggggcgctcg aaaatatcgc gatttctcgc tctctttcgt ttgctctctc
      301 gaaggtgata caggatgaaa tgggtggtac cgttgaggaa ctcgataaag aaatcggcga
      361 tcccgatact cccccagaga agcaaaaaca gctcaaggga ctgcgcgatt tctacctgcc
      421 gctcgttcgg acaggcgact actggacatt tacgtgtgac ctcctgacgt atacgattaa
      481 tgccaagctg gccgagtctc agagcaaatt tcaggaagca taccgcctct atgatgaagc
      541 tgcgcgtctt gaagaggaca tgcgaaagat aatacttgtc tggatgtcgc tttatgccaa
      601 catcagtaca ctggatggaa ttgttggttt agatgcccca caacactcga tacgatctac
      661 ctactttcaa ggaatggcgt cgctcagtcg tggagactcc gaacttatga agggggatta
      721 tgctgaagct cgcaaacatt atcagaacgc gaacactacc tttgatactg ctgcgagatc
      781 gtggacgctt caaatggagc gcctcaactc tccatcgcag atcgacggac gcgcaaacgc
      841 ggatagagag agaagactct gcctggatcg tatgcgttac tgcgatgcga aagatgagat
      901 ggcacaggct cagcggtact ctgcctacga cgaatttgct gatgcgagtg aacacttcga
      961 caacgctgcg ataatcctca atgccctttg gaaggactcc ttagacaggg aaaccccgcg
     1021 taatctcgac cttttcaaag catccgagat gttctgcaaa ggctgtgcgc tcctggagga
     1081 agcgcgtagg gtgagccgag aggatatcat gctcaaggcg aaagagccga ttgaccgcgc
     1141 cgtttcactc ttcgaggatg caagcgagcc gctctggtcg aagtacatca acgctctgcg
     1201 tctagaatac gaagcggata tttataagaa gttgtcaaac gcgagcgcat cgcagaaaaa
     1261 tcaactacag gcagccgttt atgcccaatt tgcccaaaag caggcgagcg aggcatctga
     1321 tatttttaag cagcttggtg tggagaagcg cgtatctggt atggatcgac ttgctcatag
     1381 catggctcac tcggtggttg agtcccaggc gctgtccctc ttctctcctc ccaatcctgc
     1441 ggatgctcca atggcttctt caaataatgc tgtgcagagc atgaaggatc aactgcgatt
     1501 tggcgggata caaccaggcc agattatctc agaaaatcga ctcgaaacat tcctcgctga
     1561 gatgtccgca atcgatagca atttagacga tttaaaacaa caattcggcg atggcgacct
     1621 ggatcaagct cggtacttga agatggaagc gaagcggata cagcagcgca gcgcaatttt
     1681 gcaaaaggtg cgcaaggagt tggaaggtcg cgataaagac cttgacacag tcatgcagga
     1741 tgccacgacc ggggggcagg aggagcgcgt gttgatggat cgattagcgg aagtggctca
     1801 gcggcgaggg cttacttcct cgctgatcga ctcgctcacc aggcatcagg gaacattcct
     1861 tccctcgctg attgaggttg gcaagcagct tattaagctg tcagattagc gctatttttt
     1921 ccaggtcaac cctgcgagca catcgacaaa aatgggtaat cttacgcacg taatcggcgg
     1981 gtaaatcgcc tatactctgg tcaaaggaaa ctacttttct atgtaatata gttttataag
     2041 gagtgcgaat atggtggaaa attcgtctgt gcctaacgga ccggatgcct accggtcata
     2101 tattggcgcg acgctggggg agtatcgcct ggaccaggtg cttgaagaga gcgaggttgg
     2161 tcctatcttc ctggcgcgaa cgagccaggc gtcgttccgg gttcaactgc tgactgttcc
     2221 gcccgacctg gcgtcgcagg atcgcatggt gtggctgggg cgattccagc gcgaggcgaa
     2281 tcttgtcgct tcgttgcggc atcccggact gctgcctctg ctcgattatg gcatctatca
     2341 aagtttcccc tacctggtga tgccgcgcct gaagatgaca tcgctttccg cctatctgtc
     2401 gaaacagggg gcgctggata ccctgctcgc cagtcgttac ctggatcaaa tcgcagacgc
     2461 gctggaatac gcgcacgcgc aggcggcgct tcatcttagc ctttccaccg attgcatttt
     2521 cgtcaaagag gataacagcc tggtcgtggc gaacctcggc attgtgcgaa tggtcgaaac
     2581 tgggggaaca acggcttcgc tagccgcaca agaacaggag cgcaatcact ttatcttgcg
     2641 tgacaggcag ggcagggcgc tctatggact cggcccaggt agctctcctg cgccggagca
     2701 attgctcgtc aaccccgtcg atacctacac cgatgtctat gcattgggcg ctgtgctgta
     2761 ccgtatgctg actggccacc gcgtcttccg gggcaccaac gcggcggagc tggcgcagca
     2821 gcatctcacc gcgcctgtgc cgccgctcag caagtggcgc gcgggcattg cgccgcaagt
     2881 tgatgcgctg atcgcgaagg cgatgtcaaa agagccgtcg cagcgtttcc gccagcccgg
     2941 gctggtggcg aatgcctacc atgacagtgt cgcgcccaac gatacacagc gcagggcatt
     3001 cgccgtaggc gcgccgtcgc tggtgtctac gcaggcgagt agggatgcag cgatggcggc
     3061 agctcctctt gcgccaaagc aggtcgggcc tcgcccgtcc agcgcgcgac catcagggac
     3121 gccacgcggg acagcaggtc gggcgaataa cgcgggcatc tctcgccggc gcttgctggt
     3181 cggcggcggc gctgccgttg ctattgtggc tgtgacggtc tttgccagcc attacctgat
     3241 cggcactacg actccgggtg gaacaacgac gacgaatggt ggcgggcctg gctctaccgg
     3301 tgggtcaggc gggcatgcag ggactgtgat cgcgcatacc tcggatgtgc aggtcaacag
     3361 cgcgaagact tttcctatcg cgaaccagca gaaccccggc gtcctggtgc atttacagaa
     3421 caacagtttc gtggcctttg actccacctg cacccatgct ggctgcgctg tggcatacaa
     3481 cccgcaggat cacctgctgg aatgcccctg tcatggcgca tcctttgacc cggcaaaggg
     3541 cgcaagtgtg gtgcagggtc ctgcgacgac cccgcttaca tcgatcccaa ttaaggtgaa
     3601 tgcggacaat accataacga catagggata aagacaacta tgcagaggga ggtgagcgtg
     3661 tgtgcctgag caatatgtga tcgttgggaa cggtatcgcc gggatgaccg ccgcggaaat
     3721 cttgcgcgat gaaaacgacg cggcggatat caccgtgatc gccgatgacc cgtttccggt
     3781 ctattatcgc ccggccttga aggattacct ggccgggcgt gtgcgcgagg agaagctgtg
     3841 ggcgcgcccg aatagtttct accaggatcg ccgcattcgc tttgtcgcgg agcgcgtcga
     3901 gcgcatccag gttgggcagc atgtcgcgca gcttgcgaac gggcgccagg tcggctattc
     3961 gcgcctcctg ctggccagtg gcgcgcgcgc cagccgtttg agttgtccag gcattaatct
     4021 cgcgggcgtg accacgctgc gcaccgtcgc ggactaccag gaggtgcagc agcagcttaa
     4081 ccaggcgcgc catatcgttg tgagtggcag cggtacactg gcgctggaaa cgatcgaaac
     4141 cttgcggcat aggggttttg gcgtcacgca tttactgcga aaacgcacac tatggtcgga
     4201 ggtgcgggac gcgacggcct ccgacctggt gctgcaacag gagcggcgcg atggtgtgga
     4261 tgtgcacgtc gaggaagaga tcgccgagat tacgggtaaa gatgggcggg tgacgggtat
     4321 cacgaccagc agcggcgcgc gcattccctg tgagatcgtg atcattgcaa ttggcgtcga
     4381 tcctatcgtc gattttgtga agagcagcgg cattccctgc gggcgaggca tgaaggtgga
     4441 cgcggccatg cgcagtaacg cgccggatat ctttgcggca ggcgatattc tggagacaac
     4501 cgacgcttcg actggccgca cgcgcgtgat cggccagtgg tttcccgcca ttcagcaggc
     4561 gcgcgcggcg gcctacagta tgcttgatat gctcgatacc cgcagaccct ttcaatccag
     4621 caacttctac aatgctacct tcctgtacgg gctggaattt gcctcggttg gcctcaccca
     4681 ggctccgaaa ggcggccagg gctatcaaga attggtagca gacccgctgc cgcgcacgta
     4741 tcgcaaggca ctgctgaaaa acggcgtgcc tgtcgggatg ctcatgctcg gcaaccgcaa
     4801 ggatggactg gccctcaaac gcgctatcga tcatggcgtc aacctcacac cggtggcatc
     4861 gcagctcttc gtcgagggtt tcaagcttga tgcctggctc gataagcagg gagtgcctcc
     4921 acccatattg agcgctaata aagagggcta cgttgcgaaa gaagcgccgg tcagcgatgg
     4981 cgcaacggtg gtggcagcag gggtggatag tgccgccgtc ccgcctgctc ctcggcgaga
     5041 ggcttccctt gtgccggtcg atgtgggtgc ggggggcgag gtgaagctga gcgaggagca
     5101 ggctatgacg gttgggaggc aagcaggagt cttcctgctg atcgatcacg cctcggtttc
     5161 gcgccatcat gccgaaatta cctacgtgaa tgggcagtat atcctgcgcg acctgggcag
     5221 ctcgaacggc acgtttgtca atgagacacg cctggaaatg ggcagcacac gcgcgcttga
     5281 agcagggcag cgcattcgtt tcggcaaagt ttcgtaccag gtccagatgc ggagcagcca
     5341 ggctgagcaa gcagcgccgc gcacggtatc ggcggagaca tcttacatcc gcgagttgac
     5401 aaccggtttc ttcgatccga tggcggctgg ggccattccg gcgacatcgt atcagccggt
     5461 attgaatgcc gatgg
//
LOCUS       DASZAK010000531         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_119144, whole genome shotgun sequence.
ACCESSION   DASZAK010000531 DASZAK010000000
VERSION     DASZAK010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119144"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..564
                     /locus_tag="VGT44_09300"
     CDS             187..564
                     /locus_tag="VGT44_09300"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEV2581036.1"
                     /translation="MFPSLQLLIPGVAVLLLACAILFPMFRSMFATINVRRHGTSIAA
                     TVTKIDAELVVRIAREYNTSYFIYADWEDPRTHKVYHFKSKAGSARISFLHPPGSLIE
                     VLIDPKNPRRYEVVLKFDEQGFI"
     gene            616..2337
                     /locus_tag="VGT44_09305"
     CDS             616..2337
                     /locus_tag="VGT44_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV2581037.1"
                     /translation="MPSDATREPRIRFDVRVPMRDGLTLSADIYFPPGKADEPGSYPV
                     ILTRTPYQKLSEGMLGVARYFAERGYVFVAMDVRGRGDSDGVFQPLFHEDVDGYDAIE
                     WCAAQPWSDGNVGTIGSSYPGVIQWLAALEQPPHLKAMVVRVPPSDPFVETPTGLPSP
                     MSLCWMHFVSGRYNQLMEAVDWMKVYEHLPLLTMDEQAGRFMPQWRKQIEHAQLDEFW
                     EPLRYQQQFQRVNVPVLHISGWYDDEQIGTPLNYIGMTRHAATPEARSSQRLLMGPWG
                     HVVNKDAKLGDIDFGPQALIDLRSEELRWFNRWLKGYTFHADLEESPVRIFVMGANRW
                     RDEREWPLARTQWTPYYLRSAGRANSRFGDGELSTTLPAEAEPSDTYRYDPAYPVPFI
                     TEPTSSQIGGPDDYSAIQRRDDVLVYVTGPLQEELEVTGPITLDLYASSSAPDTDFMA
                     MLLDIWPNGVSQRLCDGMVRARFRDGLDKPSLIEPGKIYRYHIDCWNTSQVFKAGHRI
                     GLQISSSAFPKYDRNLNSGALLGFTTEVAVAEQRVYHDEGHPSAIMLPIIPPAAEPDE
                     AYEVFTR"
     gene            complement(2384..>2833)
                     /locus_tag="VGT44_09310"
     CDS             complement(2384..>2833)
                     /locus_tag="VGT44_09310"
                     /inference="COORDINATES: protein motif:HMM:NF044761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2581038.1"
                     /translation="LRRFFPTLSRWKTARKDFILALMRILYADNNAIFRQKARALAVK
                     RVTPLLATVIRQGIQEDVLTTSYPEQVSEVVISLVLDFSDALAGLLLSQEPGRDEYPR
                     VERTVAAYTETIERALGAPAGSLPLADDQALGEWFVSPGDREAETKS"
BASE COUNT          584 a          835 c          818 g          596 t
ORIGIN      
        1 gcaacctccg ggagagttat gagtggatgt cagctacgtc acctttctgc gtgtcactcc
       61 ccaaaaaaga catccgcaat atctgccggg ttattgcgtc tattctagtg agataattcc
      121 gtctcaagac gtagtataga gaagcaggtt gtttgagcat tgatagaatc gcctggagca
      181 ggagctatgt ttccctcgtt gcagctactc attcccggcg tcgctgtgct gctgctggcc
      241 tgtgctatcc tgttcccgat gttccgctcg atgtttgcaa ccatcaatgt gagaaggcat
      301 gggacaagca tcgctgccac cgtcaccaaa attgacgcgg aactggtggt gcgtattgcg
      361 cgtgagtata ataccagcta tttcatatat gctgactggg aagatccccg cacccacaaa
      421 gtctatcatt tcaaaagcaa ggccggaagc gcgcgtattt cgttccttca cccccccggc
      481 agtctcatcg aggtgctgat cgacccgaaa aacccccgca ggtacgaggt tgtgttgaaa
      541 ttcgatgagc aaggcttcat atgatatgat agcgggcaaa caggcatatt tacgatagca
      601 agaaagaata gcttcatgcc ttccgatgct actcgcgaac ctcgcattcg cttcgatgtg
      661 cgcgtcccca tgcgcgatgg gcttacgctc tctgccgata tctattttcc gcctggcaaa
      721 gctgacgaac cgggcagcta cccggtcatc ctgacgcgca caccgtatca gaagcttagc
      781 gaaggtatgc tcggcgtggc gcgttacttt gccgaacgcg gttatgtctt tgtggcgatg
      841 gacgtgcgcg ggcgcggcga ttctgacggc gtcttccagc cgcttttcca cgaagatgtg
      901 gatggctacg atgccatcga gtggtgtgct gctcaaccct ggtcggatgg caacgtcggc
      961 acgatcggca gctcgtatcc aggcgtgatc cagtggctcg cggcgctgga gcagccgccg
     1021 catctgaaag cgatggtggt gcgcgtcccg ccatctgatc ccttcgtcga gacgccgacc
     1081 gggctgcctt cccccatgtc tctctgctgg atgcactttg tgagcgggcg ctacaatcag
     1141 ctcatggagg cggtagactg gatgaaggtc tacgaacacc tcccgctgct gacgatggat
     1201 gagcaggcag ggcggtttat gccgcagtgg cgcaagcaga tcgaacacgc gcagcttgat
     1261 gaattctggg aaccgctgcg ctaccagcag cagtttcagc gcgtcaacgt gccagtgctg
     1321 cacatctctg gctggtacga cgacgagcaa atcggcacgc cgctcaacta catcggcatg
     1381 acgcggcacg ccgccacacc ggaggcgcgc agcagtcagc gtctcctcat gggtccctgg
     1441 gggcatgtcg tcaacaagga tgcgaagctc ggcgacatcg actttggccc gcaggcgttg
     1501 atcgatctgc gctccgaaga actgcgctgg ttcaatcgct ggctcaaggg ctacaccttc
     1561 cacgccgacc tggaagaatc gccggtgcgc atcttcgtca tgggcgcgaa ccgctggcgc
     1621 gacgagcgcg aatggccgct ggcgcgcaca caatggacgc cctactatct gcgcagcgct
     1681 ggccgcgcca acagtcgctt tggcgatggc gagctctcga ccacgttgcc tgccgaagct
     1741 gagccgtccg acacgtatcg ctacgatccg gcctatcctg tcccgttcat caccgaaccc
     1801 acgtcgtcgc agattggcgg ccccgatgac tactcggcca tccagcgccg cgacgacgtt
     1861 ctggtctacg tgaccgggcc gctgcaagaa gagcttgaag tcaccggccc catcacgctt
     1921 gatctctacg cctcgtcttc cgcgccagac accgatttca tggccatgct gctcgatatc
     1981 tggccgaacg gcgtcagcca gcgcctgtgc gacggcatgg tgcgcgcccg tttccgcgac
     2041 ggcctggata agccatcgct gatcgagcca ggtaagatct atcgctacca catcgactgc
     2101 tggaacacct cgcaggtctt caaggctgga catcgaattg gattgcagat aagttcaagc
     2161 gcgttcccaa aatatgacag gaatttgaat tctggagcgc tacttggatt tacgacggag
     2221 gtggcggtgg cggagcagcg ggtttatcat gatgaggggc atccgtcggc gatcatgctg
     2281 cccatcatcc cgcccgccgc tgagccggac gaagcatacg aggtcttcac gcgctgatga
     2341 tattggctct ctaaacatag ggtacaggcg ttctattgtg tcattatgac ttcgtttcag
     2401 cttctctatc tcccggcgag acaaaccact ctccgagcgc ctggtcgtca gcgagaggca
     2461 aggaaccagc gggagcgccc agagcgcgct caatcgtctc agtataggcg gcgacggtgc
     2521 gctcgacgcg gggatactcg tcccgccctg gttcctgcga gaggagcagg ccagccaggg
     2581 catcactaaa gtccagcacc agagagatga ccacttcgct tacctgctca ggataggaag
     2641 tggtcaagac gtcttcctga attccctggc gaatgacagt tgcaagcaga ggcgtgaccc
     2701 gtttgaccgc cagcgcgcgc gccttctgac ggaagatggc gttgttatcg gcgtacaaga
     2761 tgcgcatcag agccagaata aagtccttcc gagcggtttt ccaccggcta agcgtgggaa
     2821 agaagcgccg gag
//
LOCUS       DASZAK010000532         3727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_226305, whole genome shotgun sequence.
ACCESSION   DASZAK010000532 DASZAK010000000
VERSION     DASZAK010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226305"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..978)
                     /locus_tag="VGT44_09315"
     CDS             complement(106..978)
                     /locus_tag="VGT44_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581039.1"
                     /translation="MSNGSDVPTHVSALYPPARRRLPPQQQRNRHAPDEPPPSRTFIP
                     GGVQPEDAPYTTGERLHWSAQRSFPAQTTAQRGSPFTPEMDAAYEEAVRHGDLFPDDW
                     AGKDGRGRRRDTTSAVRSDTTTSRPSSSPPDSAPMRAPRRPSPSPWKRALLVGMLAMG
                     ALTVSANAGYSWWQGVQADWSYGYPRVYQTDAVVGHHHDSAAHPSHFLVLNLHGQVDI
                     IELPAGDPSQALIYTGPALTGAEADTEVVTVSFQDVNHDGRPDLVLHVGATEMVLLNG
                     TDGKFHQPSQQQGQ"
     gene            complement(980..1492)
                     /locus_tag="VGT44_09320"
     CDS             complement(980..1492)
                     /locus_tag="VGT44_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581040.1"
                     /translation="MTTRSDLLPLSCEVPDAAAAAQLSTYWMPLDEPRHTWASFGDEA
                     TLNQSLIATGHAFFALAQETKALRHFTFATEQQQHPRFAKDADWWYTQAERALYEATH
                     HWSRAACALDTLRPTDLTPDALLEVRALSRQVRQQQERLWSLTDEVRAAIAAWRRQQQ
                     QQTTHPQEVV"
     gene            complement(1489..1773)
                     /locus_tag="VGT44_09325"
     CDS             complement(1489..1773)
                     /locus_tag="VGT44_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581041.1"
                     /translation="MHDLWGELSDLLLPQRSTPLVPPTQSQPIGLKQAMCAYYGHTWQ
                     RIGLSNEKQCTVCFLKGYCPGCTLVAPPDAHLFFCTKHTPASPEQSEVQP"
     gene            2196..2408
                     /locus_tag="VGT44_09330"
     CDS             2196..2408
                     /locus_tag="VGT44_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2581042.1"
                     /translation="MATLNDLITEAGLRKRELSDQSGVDVATIIRIGNGRATTRVTVV
                     KLVRVLEKYLDRSIDIDRIEGLNINK"
     gene            2627..2977
                     /locus_tag="VGT44_09335"
     CDS             2627..2977
                     /locus_tag="VGT44_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581043.1"
                     /translation="MSGKYTEFEKQTDGNLRIVLLPEARYDVQEISSQEIDADRKLSE
                     VIEWQLANGWSLVRPEDVGALTDAPLLSEDVDIDDQGTIRSVGIVYWYPQYDVSDPVE
                     QLLQQGYVDFVRGD"
     gene            3078..3422
                     /locus_tag="VGT44_09340"
     CDS             3078..3422
                     /locus_tag="VGT44_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581044.1"
                     /translation="MPADPDIPPEDMAYAAHLLRLASEERDGLSENDHESVAYVVLSA
                     LIEMFEDAANGYVTLTGLPVAIREGLEREGLITPPQKRLVGLDEQKEQERRAIYHRYL
                     KLMNEYLEARSA"
BASE COUNT          760 a         1006 c         1169 g          792 t
ORIGIN      
        1 ggccaggtat tcctcctccg tcagcgccgg gagcaggtcc agcgtctcac cggttccgtc
       61 ttgtgccatt ggtcgatcat cgcacatggt cgcttgctcc tttttctact gcccctgctg
      121 ctgggagggt tggtggaact tgccatccgt gccgttgagc agcaccatct cggtcgcgcc
      181 cacgtgcagc acgaggtcgg gtcgcccatc atggttcaca tcctggaagc tgaccgtcac
      241 gacctcggtg tccgcctccg cgccggtgag cgccgggccg gtgtagatca gcgcctggct
      301 gggatcgccc gccgggagtt cgatgatatc gacctggcca tgcaggttga ggaccaggaa
      361 atggctgggg tgcgccgcgc tgtcgtggtg atggccgacc accgcgtcgg tctgatagac
      421 gcgcgggtag ccatacgacc agtcggcctg caccccctgc caccagctgt acccggcgtt
      481 ggcgctcacc gtcagcgcgc ccatggccag cattccgacg aggagcgcgc gtttccaggg
      541 ggagggcgat ggacgcctgg gcgcgcgcat cggcgcggag tctggtggcg acgacgacgg
      601 gcgcgacgtg gtggtgtcag agcgcacggc gctggtggtg tcgcgccgcc tcccacgccc
      661 gtctttcccc gcccaatcat ccgggaagag gtcgccgtgc cgcacagcct cctcgtaagc
      721 agcgtccatc tccggcgtga agggcgagcc gcgctgcgcc gtcgtctgcg cgggaaacga
      781 gcgctgcgcc gaccagtgga ggcgttcccc ggtggtgtag ggcgcatcct ctggctgcac
      841 tccgcctgga atgaaggtgc gcgagggcgg cggctcatcg ggcgcgtgcc ggttgcgctg
      901 ctgctgcggt gggagccgcc gacgagccgg cgggtagagc gcggacacgt gggtcgggac
      961 atcggagccg ttgctcatgc tatactacct cctgcgggtg agtcgtctgc tgttgttgct
     1021 gcctgcgcca ggcggcgatg gcggcgcgca cctcgtcggt aagactccac aggcgctcct
     1081 gctgctgccg cacctgccgt gacagggcgc gcacctccag cagcgcgtcc ggggtcaggt
     1141 cggtcggtcg cagcgtatcc agcgcgcagg cggcgcggct ccagtgatgg gtggcctcgt
     1201 agagcgcacg ttcggcctgg gtgtaccacc agtcggcatc tttggcgaag cggggatgct
     1261 gctgttgttc ggtggcgaag gtgaagtgcc gcagggcttt ggtctcctgg gccagcgcga
     1321 agaaggcgtg tccagtcgcg ataagcgact ggttcagcgt ggcctcgtcg ccaaagctcg
     1381 cccaggtgtg gcgcggctca tccagtggca tccagtaggt gctgagctga gcagccgcag
     1441 ccgcatctgg gacctcgcag gagagtggga ggagatcgga gcgggtggtc atggctgcac
     1501 ctcgctttgc tcaggcgatg ccggggtatg tttggtacag aaaaagaggt gtgcgtccgg
     1561 tggtgcaacc agcgtacagc cagggcagta ccctttgaga aagcagacgg tacactgttt
     1621 ttcgttagag aggccgatgc gctgccaggt atggccatag taggcacaca ttgcttgctt
     1681 caatccaatt ggttgcgatt gtgttggagg aacgagcggt gtcgaacgtt gtgggagcag
     1741 caggtcagag agttcccccc aaagatcgtg catctgagat tcctttcttg acaccagggt
     1801 tctctcatgc gagactatgc gtatgagaac ccgactacct atcctgtgtg tgtatggttt
     1861 ctcgccttgc ccggcctaac gtgttccaga cggtaggccg gatgattgtc tcaccccccc
     1921 ctctttgctt ctgatagcgg tccgttagaa cgatgccatg aaactcttca atttggctac
     1981 tgctccgcgt ttgtggcaac actcatgcta gctgtctttc tccgaatttc acggcacgcc
     2041 tataataata ccttgtaagt catgaaaagt caatgcttgc catgatatgc tgatatatcc
     2101 attagcaatc aaggctcttt attgacaata ctgcgtccct ttggtatagt agcaacaaca
     2161 ccggtgcatt gattgacctt gtaaggaggg gaattatggc aacactcaac gatctcatta
     2221 cggaggcagg gcttagaaag agagaactat ctgaccagag cggggttgat gtggcgacga
     2281 tcatccgaat tggcaatggc agggcgacaa cgagagtgac ggtcgtcaag ctagtgcgag
     2341 tcctggaaaa gtatctagac cgaagtatcg atatcgatcg tattgaaggg ttaaatatca
     2401 acaagtaacg tgtcaaagtt tattaaggga agtaatcgac tcaccgcttg atcgtctttg
     2461 ggaaggtcgc ggtgttcgct tcgtttaccc cacgttgcgg atgcaagaag aacgtaatgc
     2521 ccgatcacct caccagggac tcgattcgat tgctggcgaa tacgctgatg ttgactgtgt
     2581 gagggatagt tcgcgattca aacagccagg gagggagata accatcatgt cagggaaata
     2641 taccgagttc gaaaaacaga ccgacgggaa cctgcgtatt gtactgctgc cggaagctcg
     2701 gtatgacgtg caggagatct cgtcacaaga gatcgatgcg gataggaagt tgtccgaggt
     2761 catcgaatgg cagctcgcca acggctggtc gctggtgcgt cccgaggatg tgggcgcgtt
     2821 aaccgacgca ccgctgctct ccgaagacgt tgacattgac gaccagggta ctatccgcag
     2881 cgtgggtatc gtctactggt atccgcagta tgacgtatcc gacccggtcg agcaattgct
     2941 acaacagggc tacgtcgatt ttgtgcgcgg tgattgagaa aaaggcggtg acaacggagg
     3001 atggccctgg gaacagcctc gatgtatgga ttacaggtga gtgcagcggc gcaacagaac
     3061 gaaaaggagc aaagaaaatg ccagcagacc ccgatattcc gccagaagac atggcgtatg
     3121 cagcacacct cctccggctc gcgagcgagg agagggatgg actttcagag aacgaccacg
     3181 agagtgtggc gtatgtcgtc ttgtcggcct tgatcgaaat gttcgaggac gccgccaacg
     3241 gctacgtgac cctcaccggc cttcccgtcg cgatccgaga gggactggag cgggaggggc
     3301 tgattacgcc gccgcagaaa cggcttgtcg ggcttgacga gcagaaggaa caagagcgca
     3361 gagcgatcta ccatcgctat ctcaaactga tgaacgaata ccttgaggct cgctctgcgt
     3421 aggtaagaac taagcagact gcctcttgct catgtatgcc ttgccgtact cgaagtagtt
     3481 cagatcttta cgaagaccag ccccgaatcc gatccaggga gtatgtactc cgtgtttcaa
     3541 gccaagtaag tgaagctagc ccaaataatc gattaaaagg ggtatgaaaa attgaacagt
     3601 acgaggtatg agtaagccct cactttttca cgattgcgct cttttgcctg ctgccatcca
     3661 aaactgctct gcttgttggg tcataggtgt ctgtcggtca agttcacgga tatcatattc
     3721 gatattt
//
LOCUS       DASZAK010000533         4950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_653176, whole genome shotgun sequence.
ACCESSION   DASZAK010000533 DASZAK010000000
VERSION     DASZAK010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4950
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653176"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..241
                     /locus_tag="VGT44_09345"
                     /pseudo
     CDS             1..241
                     /locus_tag="VGT44_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921764.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(429..1820)
                     /locus_tag="VGT44_09350"
     CDS             complement(429..1820)
                     /locus_tag="VGT44_09350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HEV2581045.1"
                     /translation="MATATNERKGYFTGWTLKESGVIAPDERLPWGQTIFVGLQHVLA
                     MFGSTVLAPLIMGFDPNTAIFFSGIGTLIFFVLVGGRVPSYLGSSFSFIAVVLAATAY
                     KGIGLNPHLGLALGGLIACGVVYGIIALIVIFTGSRWIEVLMPPVVTGAVVMVIGLNL
                     APVAINDASGTSFDTVMAIVTVLATAAVAVYAPGTLRRLPILLGGVIGYIIYVIFSNG
                     FGIGTAISFSNVSKAAWIGLPNFTTPVFNGNAMGLIAPVAIILVAENTGHVKAVAAMT
                     GRNLDKYLGRAFLGDAVATIVSGFGGGTGVTTYAENIGVMAVTRIYSSLNFIIAAGVA
                     ILLGFCPKFGALIATIPGGVLGGLAVVLFGLIAVTGGRIWVENKVDFSKSRNLVTAAV
                     ALTVGAGMISVRDVNFGLTIGGFTIGGIGVATFGSIIIYQLLRERTPQPEEAATADSA
                     IGPFPEPETGAGE"
     gene            complement(2002..2148)
                     /locus_tag="VGT44_09355"
     CDS             complement(2002..2148)
                     /locus_tag="VGT44_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581046.1"
                     /translation="MIITTDDRGDGQHRPYPRITASHADWPRVGAMLAIAWFPRAIAW
                     SSSN"
     gene            complement(2156..2449)
                     /locus_tag="VGT44_09360"
     CDS             complement(2156..2449)
                     /locus_tag="VGT44_09360"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEV2581047.1"
                     /translation="MMWGFGLGLWFLLPLLFLGRFFWIILLVLLIGSLFRRMSYRRWQ
                     TPVYAYHYGTPMSPAQASALDILRQRYARGEIDATTFDQMRERLEGSSQPRQQ"
     gene            complement(2710..3864)
                     /locus_tag="VGT44_09365"
     CDS             complement(2710..3864)
                     /locus_tag="VGT44_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV2581048.1"
                     /translation="MYASDLYPRFSSAEYARRNAAVRAAMQEADLAALIIYGTPGAYN
                     EVLYLSNFLTTRESMLVFPIEGEPTLFVQMYNHVPNARRVASIADVRWGGPDTSLAAA
                     GNLQERALAASRIGLLGALPYKSYESLKRALPHATFIDFSRQVSQLRLVKSDEEIAFL
                     RKGAEFSNLAVAALAREARPGITEYQLAAIVEGAYLGLGGSTHIHYMATTPMSNPAAC
                     VPAQSQSNRVLEKGDILITEISAHYHGYPGQILRPFAIAAPPTPPYQRMYDVAAEAFQ
                     RIAHVIRPGATSDDVLDAAEYIHTSGYTICDDLVHGFGGGYLPPILRTRRTSATPQAP
                     FTFRENMTIVIQPNIITPDERMGIQVGELMRVTRDGIESLHHYPMTFIQC"
     gene            4189..4935
                     /locus_tag="VGT44_09370"
     CDS             4189..4935
                     /locus_tag="VGT44_09370"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2581049.1"
                     /translation="MHDMDQAVPSETLESMKAYYRERAGEYDEWFYRRGRYDRGAEAN
                     VRWFVEADEVIGALDGFVMDGDVLELAPGTGIWTERLLHTAGTITAVDASPEMIAINR
                     ARIGDKHGARVRYVLADLFDWQSGRVYDGVFFGFWISHVPLERLDSFMQMLSAALRPG
                     GKLFFVDGRREPFSTAVDHRLPDEGQQRMMRKLNDGRAFDIVKNYYDPAKLSSHFAAH
                     GLDVMVRETATYFLYGYGVHQGDREGAPLP"
BASE COUNT          995 a         1236 c         1662 g         1057 t
ORIGIN      
        1 atgaggtcta tgccaacttg cagacattcg gctttacggc tcatgtgcgc cctcgaggag
       61 aagaggccaa ggcaatcaag cggaaagctg gcttcaaagc gcgtcggtgg gtggtggaac
      121 gagtacattc ctggatgaat cgtttcgccg cctgcttatt cggtgggaga aaaagcctgc
      181 gaactatctg gcgtttctcc atttcgccta tggcttgatt gcttttcggg ctgcagggtt
      241 attcggatcg gagcaagtga tcacaactga atgtggcaag gcgactgcaa gagatggccg
      301 ccaggataac gctgggatga gcgtataacg gcgttatcct ggcggccatc tcttgcagtc
      361 gccttgccac acttgtttcc acaaccctcg cacacctgct gaccaggctc aacgcgaaac
      421 gagcattctt actcccctgc tccggtctcg ggttcgggga acgggccgat ggcggagtcg
      481 gcggtagcgg cttcttcggg ttggggggtg cgctcgcgaa ggagctggta gatgatgatg
      541 gaaccgaagg ttgctacgcc gatgccaccg atggtgaagc cgccgatggt caggccgaaa
      601 ttgacatcgc gcacgctaat catgccagca ccgacagtca gggcgacggc ggcggtgacg
      661 aggttgcggc tcttggagaa gtcgaccttg ttctcgaccc agatgcgccc gccggtgacg
      721 gcgatcaggc cgaagaggac gacggcgagg ccgcccagga cgccaccggg gatggttgcg
      781 atcaacgcgc cgaacttcgg gcagaagcca agcaggatgg caacgccggc ggcgatgatg
      841 aagttgaggc tggagtagat gcgcgtgacg gccatgacgc cgatattttc ggcgtaggtt
      901 gtcacgccgg tgccaccgcc gaaccctgag acgattgtgg cgacggcatc gccgaggaag
      961 gcgcgtccca ggtacttatc caggttgcga cccgtcatgg ctgccactgc tttgacatgg
     1021 ccggtgtttt cggcgacgag aatgatggcg acgggagcga tcaggcccat ggcattgccg
     1081 ttgaagacgg gcgtggtgaa attgggcaga ccgatccagg cggctttgga tacattgctg
     1141 aatgaaatgg ctgtgcctat accaaagccg ttgctgaaaa tgacgtagat gatgtagccg
     1201 atcacgccgc ctagcaggat aggcaggcgg cgcagagtgc ctggcgcata gaccgctacg
     1261 gctgccgttg ccagcaccgt gacaatcgcc atgacggtgt caaatgaggt gccgctggcg
     1321 tcgttgatcg cgaccggagc caggtttagc ccgatgacca tgacgacagc gccggtgacg
     1381 acgggcggca tcaggacttc gatccagcgg gagccggtga agatgacgat aagggcaatg
     1441 atgccgtaca cgactccgca ggctatcaga ccgccgagcg ccagccccag gtgggggttt
     1501 agcccgatgc ctttataggc tgtggcggcc aggacgacgg cgatgaagga aaaactggag
     1561 ccgaggtagc tgggtactcg tccaccgacg agtacgaaga agataagtgt gccgatgcca
     1621 gagaagaaga tggcggtgtt ggggtcgaag cccatgatga gcggcgcgag tactgttgaa
     1681 ccaaacatgg ccagcacgtg ttgcaggccg acgaaaatgg tttgtcccca gggaagacgc
     1741 tcgtccggcg ctatgacgcc actctctttg agagtccagc cggtgaagta gcccttccgc
     1801 tcgtttgttg cagttgccat gcattatcct ccttgaaaat tggagatgag tcattcaaac
     1861 gtacagggag taaaggcatg agggtaacat ttgtgcggta cacacctcct ttctacaacc
     1921 tattgtgatg ccagaaatga ggaatgggag gaatttattg gaagtatacc atgaggatcg
     1981 gcggttgtcg atgtttacgg gttagttgct agatgaccag gcgatggcgc gcggaaacca
     2041 ggcgatggcc agcatcgccc ctaccctcgg ccaatccgcg tgggaggccg ttatgcgagg
     2101 gtaggggcga tgctggccat cgccccggtc atcggttgtt attatcacct atgcactatt
     2161 gctgccttgg ctggcttgaa ccctccaggc gctcacgcat ctggtcgaag gtggtggcgt
     2221 cgatttcgcc tcgggcgtag cgctggcgga gtatgtccag ggcggacgcc tgggcgggcg
     2281 acataggcgt accgtagtgg taggcgtaga cgggcgtctg ccagcggcgg tagctcatgc
     2341 ggcggaacag gctgccgatg agaaggacca gcaggataat ccagaagaaa cggcccagga
     2401 acagcaacgg gagcaagaac cagagaccaa gaccaaatcc ccacatcatg ggaatcctcg
     2461 ctttcttttc gtatactttc aggatcgcgg cgctttgcat tgccctggac aatgtttttt
     2521 ggcattgtcc ggcgggctgt gcaagagctt cacgattcga gttttttgtg aacgaaacgt
     2581 ttgattgttt caccagttat aacaacgtgt tataactttc tattcctata tacgatatat
     2641 cgcgtggtgg gttgcaagaa accttgcggt tgccatgccg gatacgcgcg tgcattttag
     2701 ttggagctat tagcactgga taaacgtcat tgggtagtgg tgcagggatt ctatgccgtc
     2761 gcgggtgacg cgcattagtt cgccgacctg gatgcccatg cgttcgtccg gggtgatgat
     2821 gttgggctgg atgacgatgg tcatgttttc gcggaaggtg aagggtgctt ggggcgtggc
     2881 gctggtgcgg cgtgtgcgca ggatgggagg gagatagccg ccaccgaagc catgcaccag
     2941 gtcgtcgcag atggtgtagc cggaggtgtg gatatattcc gccgcgtcga ggacatcatc
     3001 gctggtggct ccgggacgaa tgacgtgggc gatgcgctgg aaggcttcgg cggcaacgtc
     3061 gtacatgcgc tggtatggag gcgtgggagg cgcggcgatg gcgaaggggc gtagaatctg
     3121 gccggggtag ccgtgatagt gggcgctgat ctcggtgatg aggatatcgc ctttttcgag
     3181 gacgcggttg gattggctct gagccggaac gcaggcggcg ggattactca tgggcgtagt
     3241 ggccatgtag tgaatgtgcg tgcttccacc caggccgaga tacgcgccct cgacgatggc
     3301 ggcaagctgg tattcggtga tgccgggccg ggcttcgcgc gccagggccg cgacggcaag
     3361 attgctgaac tctgcgccct tgcgcaggaa ggcgatttcc tcatcgcttt tgacgagacg
     3421 caactggctc acctggcgcg aaaagtctat gaaggtggcg tgaggaaggg cgcgtttgag
     3481 cgactcgtag gatttgtagg ggagcgcgcc cagcagaccg attctgctcg cggccagcgc
     3541 tcgctcctgt agattccctg cggcagccag gctagtgtca gggccacccc agcgtacatc
     3601 ggcgatgctg gcgacacgcc gggcatttgg gacgtgattg tacatttgca cgaagagggt
     3661 tggttcaccc tcgatgggaa agacgagcat agactcgcgc gtcgtcagga agttcgagag
     3721 gtacagcacc tcgttatacg cgccgggcgt accgtagatg atgagcgcgg ccaggtctgc
     3781 ctcctgcatg gcggcgcgca cggcggcgtt gcggcgagcg tattcagcgc tggagaaacg
     3841 tgggtagaga tcgctggcgt acatggttca tccttttgag gcgtgtgcgt actatcttgc
     3901 atgtattgta cctgatagag atggcgatca ttctcggtgc tgtcatgctg agcgaagcga
     3961 agcatctctg cgcctctcga ccgagaccct tcgctgcgct cagggtgaca ttcctgaaca
     4021 tggttgtgaa aattcattat cggccagttc tatcgaccgg atcgacgagg attcgcgccg
     4081 atcaatcggc tgcgagcacg gtaatccggc tcctacgggt ctgtcgtggt tcgctgtagc
     4141 gagatggcgt atggtatagt acggggaaat ctgtcaggga gtattatcat gcacgatatg
     4201 gatcaggctg tgccttctga gacgttggag tcgatgaagg cttattaccg ggagcgcgcc
     4261 ggggagtatg acgagtggtt ttaccggcgc gggcgctatg accggggcgc ggaggcgaat
     4321 gtgcgctggt ttgttgaggc ggacgaggtg attggtgcgc ttgatggctt tgtgatggat
     4381 ggcgatgtgc tagaactggc gcctggaacg ggtatctgga cagagcggct gctgcatacg
     4441 gcggggacga taactgccgt cgatgcctcg cctgaaatga tcgcgatcaa ccgcgccagg
     4501 attggcgata aacatggggc gcgtgtgagg tatgtgctgg ccgatttgtt cgactggcag
     4561 tctgggcggg tgtatgacgg tgttttcttc ggcttctgga tttcgcacgt gccgcttgaa
     4621 cggcttgact cgtttatgca gatgctgtca gccgcgctgc gccctggcgg caagctcttc
     4681 tttgtggatg gcaggcgcga accattcagc acggcagtcg atcaccgctt gcccgacgaa
     4741 gggcagcagc gaatgatgcg caaactcaac gatgggcgcg cgtttgatat cgtgaagaat
     4801 tattatgatc ccgcgaaact ctcctctcac ttcgccgcgc atggcctgga tgtgatggta
     4861 cgggagacgg cgacgtattt tttgtatggg tatggtgttc accagggcga ccgcgagggt
     4921 gcgcccctac cctgacacca ataggaatca
//
LOCUS       DASZAK010000534         5064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_653204, whole genome shotgun sequence.
ACCESSION   DASZAK010000534 DASZAK010000000
VERSION     DASZAK010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653204"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1593
                     /gene="purF"
                     /locus_tag="VGT44_09375"
     CDS             82..1593
                     /gene="purF"
                     /locus_tag="VGT44_09375"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV2581050.1"
                     /translation="MERLHDACGIVGILAKENGEHIDVRRYLTLALTALQHRGQESAG
                     MAVYNDDGKIVNRVSMGKVREVFSDTGTGLPETRCGIGHVRYSTTGSSCVENAGPFVV
                     GEWRGKRETIAVAHNGNLVNGPDLREKLPSTYLQSTTDSEAIALLLLHAEGPTLRERM
                     IATLPMLRGAYSLVMLAEGKLYAMRDPWGMRPLCIGRIGDNWIVASESCAIDRIGATF
                     VRKVEPGEFITIDEQGMHSEMLVSLPRKSLCVFEYIYFSDATSQLNGRFTYEVREALG
                     RELAKEHPIEADLVTPVPDSSIPAALGYAEQSGIPYGQAIIKNRYSDRTFIKPDQRLR
                     ELEVDLKFNMVKPKIAGKRLIIVDDSIVRGNTMKRLVAALRHQGVKEIHLRSSSPPLR
                     HPCYFGIDIPQENELIAAGRTIQEVADYIGVDSLGYLSVNGLGRAIASASNANDTSNT
                     RNADDISDSEAHSLLHREFCYGCMEQRGWPFDPAEAHAIAPIVIERQPTRARG"
     gene            1616..2947
                     /gene="purD"
                     /locus_tag="VGT44_09380"
     CDS             1616..2947
                     /gene="purD"
                     /locus_tag="VGT44_09380"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV2581051.1"
                     /translation="MRVMVIGAGAREHALAWKLAQSRQVEKVYAAPGNPGMAEVAECV
                     AIRGSDGERLAGFAERERIDLTVIGPEVPLIDGVADVLRARGLRAFGPGAAGAALEGS
                     KAFAKQLMVDYGIPTAQHRTFYSSDRLSAARTQSVAGRNEITYSETKAALDYLEEHGA
                     PVVVKADGNAAGKGAIVALDMPTAREAVRSMMVERVFGEAGDVVVIEDYVQGDEIGST
                     AICQGTHYLPMTLSQDHKRALDHDEGLNTGGMGVFAPLPFVDKPTEQEIYRTIVAATL
                     QALKDRGIDFSGVLYSNIMLTARGPVVLEHNTRFGDPETQAFMLLLREDLVNVLEFAR
                     TLPVKLDTLWQPGYAVSLSLASGGYPGAYRSGQPIRGIEEANRLEHVHVFHAGTALRD
                     GQLVTAGGRVLSVAAYGLTLQEAVARAYDAAARISFEGMHYRRDIAHRGLR"
     gene            3150..3629
                     /locus_tag="VGT44_09385"
     CDS             3150..3629
                     /locus_tag="VGT44_09385"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEV2581052.1"
                     /translation="MSPQVVIVMGSKGDLAHAQAVVKTLKAVDIAYEMRVCSAHKATQ
                     RLLELLQEYESAGPLVYITIAGRSNALSAVVDANTRHPVIACPPYSDRFGGMDILSSL
                     RLPSGIASPTILEPDGAALLAAKILALSDKALEQRVLEYKQRLSAELAQADNEVRSL"
     gene            3626..4348
                     /locus_tag="VGT44_09390"
     CDS             3626..4348
                     /locus_tag="VGT44_09390"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV2581053.1"
                     /translation="MNSNPHDPHETFEWKIAQRPRPRQEDGPGRAGKPLRLGVLISGS
                     GTNLQALIDAIENNELPGVEIALVVSNNIDAYGLERAHQHNLQTLYLHWKNREESETT
                     LITWLNLFQVNLVVLAGWMRILSAPFLAHFPNRVINIHPAPLPDDGTGSSYVTQDGSV
                     IPVFRGLHAVKQALDAGVHVTGSSVHYVTPEVDAGPVICREEVAIQDGDNEESLHERL
                     KAVEHRLIVEAVRRLAQYTVGG"
     gene            complement(4345..4809)
                     /locus_tag="VGT44_09395"
     CDS             complement(4345..4809)
                     /locus_tag="VGT44_09395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581054.1"
                     /translation="MKLNGQSRSFVKQVQVDLLALGDADLFSTVHQWIGGSLASGGPL
                     DVPEETRLALGYTRALPDVTPLAPYLVDVPRAELVESAQWYSPSPHHLRILITAMDVS
                     GFAQHVITLAYQSLHPTHPEWYDGVTFNAHLANYLRQMRHETPGHYTGPRKL"
     gene            complement(4821..>5064)
                     /locus_tag="VGT44_09400"
     CDS             complement(4821..>5064)
                     /locus_tag="VGT44_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581055.1"
                     /translation="SSLLVESGFSGIEIVQEQVMLSLDGLRDLGNYWLFVQGALPGVP
                     PALGAEALGTNVYEVCQELGLSEVPRMWLQIIARKE"
BASE COUNT         1044 a         1483 c         1588 g          949 t
ORIGIN      
        1 aagcgtcccc gccccacatc cacaccaccc ccgcccctac ggaagataac cgcattttga
       61 tagtttgaca ggaggatgag gatggagcga ttgcatgatg cctgtggaat tgtcggcatc
      121 ctggcaaagg aaaatggcga acatattgat gtgcgccgct acctgacact ggcgctgacg
      181 gccctccagc atcgcgggca ggagagcgcg ggtatggcag tctacaatga tgatggcaag
      241 attgtcaacc gggtgagcat gggcaaggtg cgcgaggtct tctcggacac cggcacaggc
      301 ttgccggaga cgcgctgcgg catcgggcat gtgcgctact ctacgaccgg ctccagctgc
      361 gttgaaaacg cgggaccgtt cgtggtgggc gaatggaggg gcaagcgcga aaccatcgcc
      421 gtcgctcata atggcaacct cgtcaacggc ccggatttgc gcgagaagct gccctcaacc
      481 tacctgcaat ccactaccga tagcgaggcc atcgcgctgc tgctattaca cgccgaggga
      541 ccgacgctgc gcgagaggat gatcgccacg ctgccgatgc tgcgcggcgc gtacagcctg
      601 gtgatgctag cggagggcaa gctctacgcc atgcgcgatc cctggggcat gcgtcccctg
      661 tgcatcggac gcatcggcga caactggatc gtggcctccg agtcatgcgc gattgatcgc
      721 atcggcgcga ccttcgtgcg caaagtcgag ccaggcgagt tcattaccat cgatgaacag
      781 gggatgcact ccgaaatgct ggtctcgctg ccgcgcaagt cgctgtgcgt ctttgaatac
      841 atctacttct ccgacgccac cagccagttg aacggcagat tcacctacga ggtgcgcgag
      901 gcgttggggc gcgaactggc gaaggagcat cccatcgagg cggacctggt cacgcccgta
      961 ccggattcgt ccattcctgc cgcgctgggc tatgccgaac aatcgggcat tccctatggg
     1021 caggccatca tcaagaaccg ctatagcgac cgcacgttta tcaaacccga ccagcgcctg
     1081 cgcgagttag aggtagacct gaaattcaat atggtcaaac ccaagattgc cgggaaacgg
     1141 ttgattatcg ttgacgactc aattgtgcgc ggcaacacca tgaagcggct ggtggcggct
     1201 ttacggcatc aaggcgtcaa agagattcac ctgcgctcca gttcgccgcc gctgcgccat
     1261 ccgtgctatt tcggcatcga tatcccccag gagaacgaac tgatcgcggc tggccgcacc
     1321 atccaggaag ttgcggatta cattggagtg gatagcctgg gctacctgag cgtcaacggc
     1381 ctggggcgcg ccatagcctc cgccagcaac gccaacgaca ccagcaatac ccgcaacgcg
     1441 gacgacattt cagacagcga ggcccatagc ctgctgcatc gcgaattctg ctatggctgc
     1501 atggagcagc gcggctggcc cttcgacccg gcggaggcgc acgcgattgc gccgattgtg
     1561 attgaacgac aaccgacgcg ggcgcgagga tgaagcaaag aagggaggcg agcttatgcg
     1621 tgtcatggta atcggtgcgg gcgcgcgcga acacgcgctg gcctggaagc tggcgcaaag
     1681 ccggcaggtc gagaaggtct acgccgcgcc gggcaatcct ggcatggcgg aagtcgcgga
     1741 atgtgtggcc atacgcggga gcgacgggga gcgactggcc gggttcgctg agcgcgagcg
     1801 catcgacctg actgtgatcg ggccggaagt gccgctgatc gatggcgtgg ccgacgtctt
     1861 gcgcgcgcgg ggcctgcgcg cctttgggcc cggcgcggca ggcgcggcgc tcgaaggcag
     1921 caaggcgttc gccaagcagt tgatggtcga ttacggcatc ccgaccgcgc agcatcgcac
     1981 attctacagc tccgaccggc tgagcgcagc taggacacag tccgttgccg gtcggaatga
     2041 gatcacctac agcgagacga aagcggcgct ggattacctg gaagagcatg gtgcgccggt
     2101 cgtcgtgaag gccgacggca acgcggcggg taagggcgcg atcgtcgcgc tggatatgcc
     2161 cacggcgcgc gaggccgtgc gttcgatgat ggtcgagcgc gtcttcggcg aggccgggga
     2221 tgtggtcgtg atcgaggatt atgtgcaggg cgatgagatc ggctcgaccg ccatttgcca
     2281 ggggacacac tacctgccga tgacgctcag ccaggatcat aaacgcgccc tcgatcatga
     2341 cgagggtctg aacaccggcg gcatgggcgt cttcgcgcca ctgccgtttg tggataagcc
     2401 gaccgagcag gagatttatc gcacgatcgt ggccgcgacg ctgcaggcgt tgaaagaccg
     2461 gggcatcgac ttttccggtg tgctgtactc caatatcatg ctgacggcgc gcgggccggt
     2521 ggtgctggaa cacaatacgc gctttggcga tcccgaaacg caagccttta tgctgctgct
     2581 gcgcgaagac ctggtcaacg tgctggagtt cgcgcggacg ctgcccgtca agctcgatac
     2641 gctgtggcag ccgggttatg ccgtcagcct ctcgctggcg tcgggcggct acccaggcgc
     2701 gtaccgcagc ggccagccga ttcgcggcat tgaagaggcc aaccgtcttg aacacgtgca
     2761 tgtttttcat gctggaacag cgctgcgcga tgggcaactg gtcacggcgg gcgggcgcgt
     2821 cctcagcgtc gccgcctatg gcctcacttt gcaagaagcc gtcgctcgcg cgtatgatgc
     2881 ggcagcgcgc atctccttcg aggggatgca ctatcgtagg gatatcgcgc atcgcggctt
     2941 acgatgagcg gtacaataaa tgtaggtgcc gatttatcgc gcacaccgcc gatgatgaac
     3001 ttttacgacc aagatcagcc atgtcatcct gagcgaagcg aaggatctcg gtcctggcga
     3061 atgagatcct tcgcttcgct caggatgaca tggccgttgg tggttatgaa atctcatgat
     3121 cggcctcgat tatcaggaga ggagagagca tgtccccaca agtcgtgatc gtcatgggca
     3181 gcaaaggcga cctggcgcac gcccaggccg ttgtgaaaac gctcaaggcg gttgatatag
     3241 catatgaaat gcgtgtctgt tccgcgcaca aggccacgca gcgcctgctg gagctgttgc
     3301 aggaatacga aagcgccggt cctctcgttt atattaccat cgcgggccgc tccaacgcgc
     3361 tctccgccgt tgtggacgcc aacacgcgcc acccggtgat cgcctgcccg ccctacagcg
     3421 atcgcttcgg cggcatggat atcctctcct cgctgcgcct gccgtcgggc atcgcctcgc
     3481 cgacgattct ggagcctgat ggggccgcgc tgctggcggc gaagatattg gcactctccg
     3541 ataaggcatt ggagcagcgc gtactggagt ataagcagcg cctgtctgct gaactggcgc
     3601 aggcggacaa cgaggtgcga tcattgtgaa cagtaatccg cacgatccac atgaaacgtt
     3661 cgagtggaag attgcacagc ggccacgtcc ccggcaggaa gacgggccgg ggagggccgg
     3721 caagccgctg cgcctgggtg tgctgatctc cggctccggc acgaacctgc aggcgttgat
     3781 cgatgctatc gagaacaacg agttgcccgg cgtggagatc gccctggtgg taagcaacaa
     3841 tatcgatgcg tatggcctcg aacgcgccca ccaacataac ctgcaaacgc tctacctgca
     3901 ctggaagaac cgcgaggaga gcgaaacaac gctcatcacc tggctcaacc tcttccaggt
     3961 caacctggtg gtgctggccg gctggatgcg tatcctcagt gcgcccttcc tggcgcattt
     4021 cccaaatcgt gtcatcaaca ttcatcccgc gccgctgccc gacgacggca ccggaagttc
     4081 ttacgtcacc caggatggct ccgtcattcc cgtctttcgt ggcctgcacg ccgtcaagca
     4141 agcccttgat gccggtgtac acgtgaccgg cagcagcgta cattacgtca cgccggaggt
     4201 agacgccggg ccggtgatct gccgcgaaga agtggcgatc caggatggcg acaacgagga
     4261 aagcctgcac gagcggctga aagcggtgga acaccggctg atcgtcgagg ccgtgaggcg
     4321 gttggctcaa tacactgttg gtggttaaag tttgcgtggg cccgtgtagt ggcctggtgt
     4381 ttcatgccgc atttgacgca gatagttggc aagatgcgca ttgaaggtca ctccatcgta
     4441 ccattcagga tgggttggat gaagcgattg gtaggcaagg gtgatgacat gttgagcaaa
     4501 accagacaca tccatagccg tgataagaat gcgaaggtga tgtggggaag gcgaatacca
     4561 ttgtgctgac tccacaagct ctgcgcgagg cacatcgacg aggtagggag cgagcggtgt
     4621 gacatctgga agggcgcgag tatagcctaa tgccaggcgt gtctcttctg gtacatcaag
     4681 cggtccacca gatgcgaggc tcccacctat ccactgatga acggtgctaa acaggtcagc
     4741 gtcacccagc gctaataagt ccacctggac ctgtttcaca aaggatcgag attgaccgtt
     4801 taacttcatc ggatttcgcc ttattccttc ctggcaatga tctgcaacca catgcggggt
     4861 acctcgctca gaccgagttc ctggcagacc tcgtagacat ttgtgccgag cgcttcggct
     4921 cctaatgccg gcggcactcc aggcaatgcc ccctggacga agagccagta gtttccgagg
     4981 tctcgtaagc cgtccagtga caacatcacc tgttcctgga cgatttctat accgctaaat
     5041 ccactctcca cgagcaggct gctg
//
LOCUS       DASZAK010000535         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_618098, whole genome shotgun sequence.
ACCESSION   DASZAK010000535 DASZAK010000000
VERSION     DASZAK010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618098"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VGT44_09405"
     CDS             complement(<1..352)
                     /locus_tag="VGT44_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581056.1"
                     /translation="MTTNIPGYLADELKPFGLVNSNHQLGELFAGVVGGALFPGDVQR
                     AWNYFGANTLQRLLAFVGSSTTLGVTEYGIFAASAFLYQRVLELCVGERFLDAACNGG
                     FLSLLLAERVPFVRE"
     gene            371..520
                     /locus_tag="VGT44_09410"
     CDS             371..520
                     /locus_tag="VGT44_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581057.1"
                     /translation="MHQDRLGLFAAGEEPEVLCLLELVAPFGETGFKSGAVGPGAVHH
                     DHLSD"
     gene            complement(626..1990)
                     /locus_tag="VGT44_09415"
     CDS             complement(626..1990)
                     /locus_tag="VGT44_09415"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV2581058.1"
                     /translation="MRDIHDPPAQLEVHDPDILATARAVLDACRSPREMQEVVLSAVA
                     RNEEWRGRQCINLLAPEAPTSPTVRALLSSEVGIRAAEGHIGPVNRWFAGTQHIDEIE
                     ALCVELLKKVFRARYADHRLVASMVGNLAVYTAMTEPGDVIMSLSQPFGGHSSNRSDG
                     PAGVRGLKIVDISFDPVELVVDLDLFRKVAPLVRPKVVALGASMTLFPFPLQAMQEVI
                     AEWGGRLFFDGAHQLGLVVGGQFQDPLREGAAVMTGSAGKTFSGPQSGIIVWDDPALT
                     GPLTHAIFPVLAATHQVNRVAALAASAAELLAYGRVYMAQIVRNAQALGAALDRRGIP
                     MLGAHKGYTTTHQVIADVRQFGGGLEVAQWLAQANIITNKNLIPSDRPEDWDRPGGLR
                     LGTIEITRLGMGENEMETIADFIARILVERAEPEAVRGEVIAFRQPYQTLYYCFEHGL
                     PGQA"
     gene            complement(2183..2290)
                     /locus_tag="VGT44_09420"
     CDS             complement(2183..2290)
                     /locus_tag="VGT44_09420"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEV2581059.1"
                     /translation="MEAGRVMDIELVDNLIIGQQRFVSLKEILKREYGE"
     gene            complement(2294..2518)
                     /locus_tag="VGT44_09425"
     CDS             complement(2294..2518)
                     /locus_tag="VGT44_09425"
                     /inference="COORDINATES: protein motif:HMM:NF042609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HEV2581060.1"
                     /translation="MQHFGPQELSTAELLAIILHTGTLGGSVLKLANRLLAKSGGLAG
                     LVRADFRELCAEHRIGEAKSAQVKAALEIG"
BASE COUNT          531 a          880 c          868 g          555 t
ORIGIN      
        1 cctcgcgcac aaacggtacg cgctccgcca acagcagcga caagaacccg ccgttgcagg
       61 cggcgtcgag gaagcgctcc cccacgcaga gttctaagac gcgctggtag agaaacgccg
      121 aggcggcaaa gataccgtac tcggtcacgc cgagcgtcgt ggacgacccg acgaaggcca
      181 gcagccgctg cagggtattg gcgccgaaat aattccaggc gcgctgcaca tcgccgggga
      241 acagcgctcc ccccacgacc ccggcgaaga gttcgcccag ttgatggttg ctgttgacca
      301 ggccaaacgg cttcagttcg tccgcgagat agcccgggat gttggtcgtc atttcctcgg
      361 gcgcgaacca atgcaccagg atcgtcttgg gctcttcgct gcgggtgaag agccagaagt
      421 gctttgtctc ctggagttgg tagccccctt cggcgaaacg ggctttaaga gcggtgcggt
      481 tggcccggga gccgttcacc acgatcacct ctccgactag gacgctccgt tcatgaatgg
      541 gaatcctgct ggtttgccct ccacgccttg ctgtgccgga atgctgctcg atcatcctgc
      601 ctgctttcct tccgcaaaga gagtgctatg cttggccggg caggccgtgc tcgaaacagt
      661 agtagagcgt ctggtagggc tgccggaagg cgatcacctc accgcgcacg gcctctggct
      721 cggctcgctc gaccaagatg cgtgcaatga agtcggcgat agtctccatc tcgttctcgc
      781 ccatgcccag acgcgtgatt tcgatcgtgc ccaggcgtag cccaccgggg cggtcccagt
      841 cctccggtct atcagagggg atcaggttct tgttggtgat gatgttggcc tgcgccagcc
      901 actgtgccac ctctagtccg ccgccgaatt ggcgcacatc ggcgatgacc tggtgggtgg
      961 tggtgtagcc cttgtgcgcg ccaagcatgg gaatgccgcg ccggtcaagg gccgcgccca
     1021 gcgcctgggc gttgcgcacg atctgggcca tgtagacccg tccataggcg agcagttcgg
     1081 cagcggaagc cgccagagcc gccacgcggt tgacctgatg ggtagcggcc agcaccggga
     1141 agatggcatg cgtgaggggc ccggtgagcg ccgggtcatc ccacacgatg atgccgctct
     1201 gcgggccgct gaaggtcttg cccgccgaac ccgtcatcac cgccgccccc tcgcgcagcg
     1261 ggtcctggaa ctggccgccg actaccagcc ccaactggtg cgcgccatca aagaagaggc
     1321 gcccgcccca ctcggcgatc acctcctgca tggcctgcag tggaaagggg aagagcgtca
     1381 tggaggcgcc cagagcgacg actttgggcc gcaccagcgg ggccactttg cggaagaggt
     1441 cgagatcgac cacgagttcg actggatcga aggagatgtc cacgatcttc aggccgcgca
     1501 cccctgccgg gccatcgctg cggttgctcg agtggccgcc gaagggctgg gagaggctca
     1561 tgatgacatc gcccggttcg gtcattgccg tgtagaccgc aaggttgccc accatgctcg
     1621 ccaccagtcg atggtcggca taccgtgcgc ggaagacctt cttcagcagc tcgacgcaca
     1681 gcgcctcgat ctcgtcgata tgctgcgtgc cagcgaacca gcgattgacc ggcccgatgt
     1741 gcccctcggc agcgcggata cctacctcgg aggagagcag cgcgcgtacc gtcgggctgg
     1801 tgggtgcctc gggcgcaagc aggttgatgc actgccgccc gcgccactcc tcgttgcgcg
     1861 ccactgctga gagcaccacc tcctgcatct ccctgggaga gcggcaggca tccaggaccg
     1921 ccctggcagt ggccaggatg tcagggtcat gcacttcaag ctgcgcaggt gggtcatgaa
     1981 tgtcacgcat gagacgggct cctctctcat caagttgctc aatagcactc atcggtaaca
     2041 ctgttttctc tcaagatacc acatggcgca gcactttact gtgaatccgt ctcccaatat
     2101 gggttctttc cccacagagc gctcattggg tgaacgtggc agcttgtgtg ggggaccgag
     2161 gagcaagcag gcgtatccca ccttactcac cgtactcccg cttgagaatc tctttgaggc
     2221 tgacgaagcg ctgctggccg atgataaggt tatcaaccag ctcgatatcc atcacgcggc
     2281 ccgcttccac cagctacccg atctctagcg ctgccttgac ctgggccgac ttggcctcgc
     2341 cgatgcgatg ttcggcacac agctcgcgga aatcagcacg cacaaggcca gccaggccac
     2401 cggacttcgc taacagccgg ttggccagtt tcagcacgct gccgccaagt gtgccggtgt
     2461 gcagaatgat tgccagtagt tcggcggtcg agagctcctg ggggccgaag tgctgcagcc
     2521 gttagcgcag gcgctcatgg ctcggcagtt ctcgtaccgt ggcatggtac ttgatccggt
     2581 cttgcttata tgagccagcg gtttattcca gcgggagcat ggcgcagtgt tcctccttgt
     2641 ttgtgtgagg tacttctccc ttcattattc tgctagaggt cgttaaaaaa ggaagcagga
     2701 gagatgtgca tttctcctgc ttgccacaat ttctggtacc tgatagaaag aagccctccc
     2761 atgaaggcac aagtaacttg attatgaatt aaggggtgtt aaagagcagt agcgctatct
     2821 gtaatcttca tttc
//
LOCUS       DASZAK010000536         6831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_155601, whole genome shotgun sequence.
ACCESSION   DASZAK010000536 DASZAK010000000
VERSION     DASZAK010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155601"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1144
                     /locus_tag="VGT44_09430"
     CDS             <1..1144
                     /locus_tag="VGT44_09430"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2581061.1"
                     /translation="TQGSRNKPVHSGSPAASNSPVNPSPVVSPTNVHTPRPTVTKTPI
                     NTTPTPHPTPKPTQPPTPRPTPQPTQPTPTPTQPPTPTPGTTLYTFTGHSKGIRVLQW
                     SPDGSRIATGSNDYTAKVWGSTNGSNVVTYTGHTWYVEGIAWSPNGSQIASGSADTTV
                     QIWNPNSAAHSYTYTGHSLWVNRVSWSPNGQYIVSCEQASKTGYVVQARVWDAATGNT
                     PVVYTGHTNGIFSVAWSPDGTRIASCGYDGTLQIWEAMTGNHIATFNAGVFLYGLSWS
                     PDSTHVVVGGGSTYFWVIDAGTGNSSSRYNVNANGVSDAGWSPDGTRIVAGTGGQGAQ
                     IFDYATGAKTYTYNQSGSLDGIGWSPNNQMVASGDGATGVVQVWQA"
     gene            complement(1149..1658)
                     /locus_tag="VGT44_09435"
     CDS             complement(1149..1658)
                     /locus_tag="VGT44_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2581062.1"
                     /translation="MSQANLKNPKVEAIRRDLRQSYEALKQLVAGSLAQLSEERLYVS
                     PGGEEWTIMENLAHIVELMYYWADEIKVLVAQPGKNFGRTQQHEGRLSAIREHRRDSL
                     GQVSAALPGSYAYLDGVLADLNDSDLGVTGVHVRYGEQTLAWFIEEFVTGHMQAHVGQ
                     VQAALAALG"
     gene            1798..2757
                     /locus_tag="VGT44_09440"
     CDS             1798..2757
                     /locus_tag="VGT44_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEV2581063.1"
                     /translation="MHQHIPTFLDVLEAQKRIRPYLARTPLHRYPALDALLGTEVYVK
                     HENYQPVGAFKVRGGINLISQLRPYERARGVIAASTGNHGQSVAYAARLFGVHAIIVV
                     PENANPGKVAAMKGMGAEVIEHGVHYDESKLHCETLAHEHGYRYIHSGNEPMLIAGVA
                     TEALEMLEDEPGLEVIFVPIGGGSGAAGVSIAAHAINPAIQIIGVQSEAAPAAYETWR
                     QHRLVTAPNRTFAEGLATGVAFELPQEIMREHLSNFLLVSEDEIKQAMVWMIERAHTL
                     AEGAGAASLAGAYRERESLQGKKIGLVCSGGNTSLEHLRLALA"
     gene            3182..3868
                     /locus_tag="VGT44_09445"
     CDS             3182..3868
                     /locus_tag="VGT44_09445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2581064.1"
                     /translation="MVRQSWSDEDLAKRLQERDLDAFETLIARYSREIFYFIRLVLDG
                     IGVVQDAEECVNDLFITVWQEIDSFDSERGTLHTWLTMRAKYIALDRRRQLCRRQSLH
                     IQSADEYRQWSASDTHGSGRNVPGMGASDENRVTLPPHPEASMETLLEQSERREELRL
                     ALATLPELDRFLIYQRYYKFASTEELAAKTGLTRHAVDTRLWRARKSLREALREYAHE
                     YERVQQPKKS"
     gene            3831..5045
                     /locus_tag="VGT44_09450"
     CDS             3831..5045
                     /locus_tag="VGT44_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581065.1"
                     /translation="MNMSESNNRKNHEEEDSSSLHVMHTSLPADFSQDDLEFAQELNT
                     LLSPQDEELPPYYVQTLMAAEDPRYQPVDQNFTLMTSARVFRSLHLRRRLFPTRRSLP
                     VAFSDAFRDAFSRKAALVWIATFMLIMLCTVAFTAPSFERGMVYLLQGTRSGTMGVHK
                     FPDHVKHSPYFADADFSSAPPQISLLATQQQLHFKMYWPQFIPAKYQLSSINIYEDPG
                     NTWADGPILELVYSLIGTTAPKGTGEIVIREFLPVDEVLQVVQDGSAHPIGIDRNGNA
                     KAIYVEGQWLSRGKMSPSIWSSIGRRELISQQNGVVFWIAGDQLDGINLQTLMSISQS
                     MGPFTFSSPSFIKENSVTVLQADDSSVGPFANDVLAIFSNDGVTGPYYIDVSSYVAAI
                     NASAGKITTHGH"
     gene            5035..6747
                     /locus_tag="VGT44_09455"
     CDS             5035..6747
                     /locus_tag="VGT44_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2581066.1"
                     /translation="MATEDSRGILLLPLLFFLLLACNAPPAPQVQSSSSPLLGPYTST
                     PPTAQGPRGTLVFADRQFPDTVNPLFAGSSVDLEVETALWGAPVVFDDHFHAQPDELS
                     EVPLPENGDVRDGGLTIIMHLRHDLHWSDGQPILARDFQYWWQLDQNPTTGALSTTGY
                     DQIASIETPDDYTVILHMKHPYGPYLSYLPLAAPYHAWSNLRPIDLQNDRQVLLTPTV
                     TSGPYKLAGFVDGQSYSFTPNTLYRSTTFHGPWLAHLTFRAYSSASTLLAAIQAHQVD
                     IIEGLMEDELPTFAHLPTGYRLLTAPAASYEHLDFNMSNPLFQDVRVRRAIQLAINTC
                     AILRDVLRMSDCSRAASQVEPPPSLYYDPALQPVAFDPGLATRLLAQAGWNPGTDGLL
                     TRHGQIFTIRLVTTAGNPLRAATARLIQQALRAIGIQATISSHDLQSFFAIYTRGGIL
                     ATGNFDLAMFGYQNSPEPDDEYGVFHSSQIPTANLPTLGNYGRISDPIIDSSLSAGRA
                     TVTFIQRLQDYHTFLERLAAQVYIIPLYTDLNAVILSNHAQNFLPNPNVLANNWNISD
                     WWTG"
BASE COUNT         1480 a         2269 c         1698 g         1384 t
ORIGIN      
        1 cacacaggga tcgcgcaaca agccggttca tagcggttcg cctgctgcct ccaactcgcc
       61 cgttaatcca tctccggtag tctcaccgac caacgtacat acgccgaggc ccaccgtgac
      121 aaaaacgccc atcaatacta cacccacccc acacccaacg ccgaagccca cccagccgcc
      181 tacaccacgc ccaacaccgc agcccaccca gccgaccccc acgcccacgc aaccgcctac
      241 acccacaccg ggtacgacac tctacacctt caccggccat tcaaagggca tacgagtgtt
      301 acaatggtcg cccgacggca gccgcattgc aacggggagc aatgactaca ccgcgaaggt
      361 ctggggctcg accaacggca gcaacgttgt tacgtatacc ggccacacct ggtatgtcga
      421 aggcattgcc tggtcgccga atgggtcgca gatcgcttca ggtagcgcgg atacaaccgt
      481 acaaatctgg aacccgaact ccgccgccca ctcctacacc tacaccggtc atagcctgtg
      541 ggtcaaccgc gtctcctggt ctccgaatgg gcaatatatc gtctcttgcg agcaggccag
      601 caaaacgggt tacgtagttc aggccagggt atgggacgcc gcgacaggca atacaccagt
      661 ggtttacacc ggtcatacca acggcatctt ctcggtcgcc tggtcgcccg atggcacgcg
      721 cattgcttcc tgcggatacg atggcacgct ccagatctgg gaagctatga cgggaaatca
      781 tattgcaacc ttcaatgcag gcgtctttct ctacggcctt tcctggtctc ccgatagtac
      841 gcacgtcgtt gttggagggg gatcgactta cttctgggta atagatgccg gaacaggcaa
      901 tagtagctct cggtataatg tcaatgcaaa cggagtttca gacgcaggtt ggtctcccga
      961 tggcacaaga attgtcgctg gaactggcgg tcagggcgca cagattttcg actacgccac
     1021 cggcgccaaa acgtatacgt ataaccaatc ggggagtctt gacggtattg gttggtcgcc
     1081 caataaccag atggtcgcat cgggcgatgg ggcaaccggc gttgtccagg tatggcaggc
     1141 ataggaccct acccaagcgc cgccagcgcc gcctgcacct gccccacgtg cgcctgcata
     1201 tgtcccgtca cgaactcctc gataaaccag gccagcgtct gctcaccgta acgcacatgt
     1261 acgccagtga cccccaggtc gctatcattc aggtcggcca gcaccccgtc caggtaagca
     1321 tagctgccgg gcagggccgc gctgacttgt cccagcgaat cgcgcctatg ctcgcggatc
     1381 gcgctcaacc ggccctcgtg ctgctgagta cgtccaaagt tcttccccgg ctgagccacc
     1441 agcaccttga tttcatcggc ccagtagtac atcaactcga cgatatgcgc caggttttcc
     1501 ataatcgtcc actcttctcc gccgggcgag acatacagac gctcctcgct caactgcgcc
     1561 agcgacccag caaccagctg cttcaacgcc tcgtaagatt ggcgaagatc gcgccgaata
     1621 gcctccacct ttggattctt gagattggcc tgtgacatat ctacgctatt gcccctttac
     1681 ttttccacaa ttttccttca ttatgcatta tgggggttca aacgacaacc ctcccttaat
     1741 tatcttgtgg ttgattgctt acatccgttt cgcattggga aaggcagaca cacgaccatg
     1801 catcagcaca tacccacctt ccttgatgta ctggaagcgc aaaagcgcat tcgtccctac
     1861 ctggcgcgca cgccactgca tcgctatccg gcgctggacg cactgcttgg cacagaagtc
     1921 tatgtcaaac acgaaaatta ccagccggta ggcgcattca aggtgcgtgg cggcatcaac
     1981 cttatttcgc aactgcgtcc ttacgaacgc gcgcgcggcg tcatcgcagc ctcgaccggc
     2041 aatcacgggc aatccgtcgc ctatgccgcg cgtctcttcg gcgttcacgc catcatcgtc
     2101 gtgccggaaa acgccaatcc gggcaaggta gcagccatga agggcatggg tgcggaggtc
     2161 atcgaacacg gcgtacacta cgacgagtcc aagctgcatt gcgaaacgct ggcgcacgag
     2221 catggctatc gctacatcca ttcgggcaac gagccaatgt tgatcgcggg cgtcgccacc
     2281 gaagccctcg aaatgttaga ggatgaaccg ggactggaag tcatcttcgt ccccattggc
     2341 ggcggcagcg gcgctgccgg cgtctccatc gccgcccacg ccatcaatcc ggccattcag
     2401 atcatcggcg tgcaatccga ggccgcgcca gccgcctacg aaacctggcg acagcatcgc
     2461 ctggtgactg ctcccaatcg cacctttgcg gagggactgg caaccggcgt cgccttcgag
     2521 ctgccgcagg agatcatgcg cgaacacctg agcaacttcc tgctggtcag cgaagacgag
     2581 atcaagcaag ccatggtatg gatgatcgaa cgcgcccaca ccctcgccga aggcgcaggc
     2641 gcggcttccc tggcgggcgc ataccgcgag cgcgaatccc tgcaaggcaa aaagatcggc
     2701 ctggtctgct ccggtggcaa cacctccctc gaacacctgc gcctcgcgct cgcctaacgg
     2761 aatggctcag agggcgacca caagggtacg cccttaccat gacatcgaag tcagtgcgct
     2821 catctgtatt cgtcgcgtca tggtaagggc gtacccttgt ggtcgccctc ctttacgtga
     2881 ttaccctgcg cttcgcgctc gcctgatcct cgcgcttgac ttttttgaca taatcgcgca
     2941 aaattgaaac aagccttcgc aacttcgtga gtttatcggg cgttggttcc taatgtagta
     3001 cttttgagag attagcgccc acacatgcgt atatcttgca tacgaaggcg agtgcaccac
     3061 cagggtagca tagctcttat cgacaattat ttaaccagaa cttaactgaa tcgcaacgtc
     3121 gggtaggtgg ccgacgtgtt acgatatgag cgtgatgccc aggtctctgc aaggagtcgt
     3181 catggtacga cagagttggt cggatgaaga cttggctaaa agacttcagg aacgcgacct
     3241 ggatgctttt gagacactca tcgcgcgcta ctctcgcgaa atcttttact ttatacggct
     3301 ggtgctggat ggcatcgggg ttgtacagga tgctgaagag tgtgtcaacg acctcttcat
     3361 caccgtctgg caggagatcg atagtttcga ttcagagcgc ggcaccctgc acacctggtt
     3421 gacgatgcgc gccaagtaca tcgccctcga ccgccgccgc cagctttgtc gtagacaatc
     3481 attgcacatt caatctgccg atgagtaccg ccaatggtcg gcctctgata ctcatgggag
     3541 tgggcgaaat gttcctggca tgggggcgag cgacgaaaat cgcgtcactc tgcctcctca
     3601 cccagaggcc agtatggaaa cactactaga gcaaagtgag aggcgcgaag aactgcgcct
     3661 tgccctggcg acgctgcctg agcttgaccg ctttttgatc taccaacgct attacaaatt
     3721 cgccagcacc gaagaactgg cagcaaaaac gggcctgacg cgccatgctg tcgatacccg
     3781 gctctggcga gcgcggaaga gcttgcgcga ggcgctcagg gagtatgcac atgaatatga
     3841 gcgagtccaa caaccgaaaa aatcatgagg aagaagactc ttcatctctt cacgtcatgc
     3901 atacatcgct tccggctgat ttcagtcaag atgacctgga gtttgcgcaa gaactgaata
     3961 cgctcctctc tccccaggac gaagaactgc cgccctatta tgtgcaaacg ctgatggccg
     4021 ccgaggaccc tcgctaccag ccggttgacc aaaactttac cctgatgacc agcgcgcgtg
     4081 tcttccgttc gcttcacttg cgccgccgtc tcttcccgac ccgccgctcg ctgccagtcg
     4141 cattctccga tgccttccgc gatgctttct cgcgcaaagc ggcactggtc tggattgcca
     4201 cctttatgct catcatgcta tgtaccgtcg ccttcacagc gccatccttc gagcgcggca
     4261 tggtatatct cctgcaagga actcgcagcg gcacgatggg ggtacataaa ttccccgacc
     4321 atgtcaagca ttcaccatac ttcgccgacg ccgattttag ctccgcaccg ccgcagatca
     4381 gcctgcttgc cacacagcaa caactccatt tcaaaatgta ctggccacag tttattcccg
     4441 caaaatacca gctgagcagc atcaatatct atgaagaccc cggtaatacc tgggctgatg
     4501 ggccaatcct cgaacttgtc tacagtctca tcggcacaac agcgccaaag gggaccggcg
     4561 aaatcgtcat tcgcgaattt ttgccggtgg acgaggtgct gcaggtcgta caggacggtt
     4621 cggcgcaccc tatcggcatc gaccgaaacg gcaacgctaa agctatctac gtcgaaggcc
     4681 agtggctatc acgcggcaaa atgtccccgt ctatctggag cagcattgga cgccgagaac
     4741 tcatctctca gcagaacggt gtcgttttct ggatcgcggg cgatcagctt gacggtatca
     4801 atctgcaaac cttgatgtcc atttcccaat cgatgggtcc cttcacattc tcaagcccat
     4861 cattcatcaa agaaaactcg gtcacagtcc tgcaggctga cgactcctcc gtcggtccat
     4921 tcgccaatga tgtgctggca attttctcca acgacggcgt cacaggcccc tattatatcg
     4981 atgtgagttc ctatgtcgcg gcaatcaatg cctcggcagg gaaaatcact acgcatggcc
     5041 actgaggata gccggggcat cctgttgctc cctttgctgt tcttcttact gctcgcctgc
     5101 aatgccccac ctgcgcccca ggtgcaatcg tcatcctcgc cgttgctcgg accttatact
     5161 tcaacgccgc caacagcgca agggccacgc ggcacactcg tctttgcgga tcgccagttc
     5221 cccgatacag tcaacccctt attcgccggt tcgtccgttg acctcgaagt cgaaacagcg
     5281 ctgtggggcg cgccggtcgt atttgacgat cactttcacg cccagcccga cgagttgagc
     5341 gaagttccct tgcccgaaaa tggcgacgtg cgcgacggcg gcctgacaat catcatgcac
     5401 ctgcgtcacg acctgcactg gtcggacggc cagcccatcc tggcgcgcga cttccaatac
     5461 tggtggcagc tggatcagaa ccccactacc ggcgcgctct ccaccaccgg ctatgaccag
     5521 atcgccagca tcgagacccc cgatgactat accgtcatac tgcacatgaa acatccctat
     5581 ggtccctacc tgtcctatct cccactggcc gcgccctacc acgcctggag caatctgcgc
     5641 ccgattgacc tgcaaaacga ccgccaggtc ctcctcaccc ccaccgtaac cagcggcccc
     5701 tacaagctcg caggcttcgt cgatggccag agctacagct tcactcccaa cactctctat
     5761 cgctccacga cctttcacgg cccatggctc gcccacctca catttcgtgc ctatagcagc
     5821 gcctccacct tgctcgcagc aatacaggca caccaggtcg atatcatcga aggcttgatg
     5881 gaagacgaat tgcccacctt cgcgcatctc ccgaccggct accgcctgct gaccgcgccc
     5941 gccgcttcct acgaacatct cgacttcaac atgtcaaacc cattattcca ggatgtgcgt
     6001 gtgcggcgcg ccatccagct cgccatcaat acctgcgcca tcctgcgtga cgttctgcgc
     6061 atgtccgatt gctcgcgcgc cgcctcgcag gtcgagccgc cgccatcgct ctactacgat
     6121 cccgccctcc agcccgtcgc gtttgaccct ggcctggcaa cacgcctgct ggctcaggcg
     6181 ggctggaacc ccggcacgga cggactgctg acccggcacg gccagatctt cacaattcgc
     6241 ctggtcacaa ccgccggcaa tcccctgcgc gccgcgaccg ctcgcctcat ccagcaagca
     6301 ttgcgagcca tcggcatcca ggccaccatc tcctcccacg acctgcaatc tttcttcgcc
     6361 atctacacca ggggcggcat cctggcaacc gggaattttg acctggccat gttcggctac
     6421 cagaacagcc ccgaacccga cgacgagtac ggcgtcttcc actcgtccca gattcccacc
     6481 gcgaacctcc ccaccctggg caactatggc cgcatctccg accccatcat cgactcatcc
     6541 ctctcagctg gccgcgctac cgtcactttc atccagcgcc tccaggatta ccataccttc
     6601 ctcgaacgcc tggccgcgca ggtctacatc atccccctct ataccgatct caacgccgtc
     6661 atcctcagca accacgcgca gaacttcctg cccaacccca acgtcctcgc caacaactgg
     6721 aatatcagcg actggtggac gggatagcca gggttcaatc gaccggtgta gggacgcaat
     6781 tcattgcgcc cagggaagac acatcacccg gaacgagggc gcaatgaatt g
//
LOCUS       DASZAK010000537         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_296663, whole genome shotgun sequence.
ACCESSION   DASZAK010000537 DASZAK010000000
VERSION     DASZAK010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296663"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VGT44_09460"
     CDS             complement(<1..464)
                     /locus_tag="VGT44_09460"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2581067.1"
                     /translation="MPGSLRLGKIAGIDISIHVSWLLIVVLLTWSLATGWFPALYPGW
                     STLTYWFVSLLATLLLFASVLLHELAHSLVARARGLPVKNITLLIFGGVSNIEQEPKS
                     PGVEFQMSFVGPLTSLLLGGLSYLLLLVVRGGTSPLSAILGYLAVANVLLGI"
     gene            complement(478..1413)
                     /locus_tag="VGT44_09465"
     CDS             complement(478..1413)
                     /locus_tag="VGT44_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2581068.1"
                     /translation="MFQNILVPLDGSTRAEQALPVAERLARAAKGRLLLARVVNPPID
                     YSGGLAPVPLMTDQILESEMAGATDYLTAVAARPALSGIEIMTEVLYGFPGQQLIESI
                     EAHGTDLVVLCSHGRTGLSRWALGSVAHTLVHQSAAPVLILRQEGTPARVEAARSIRT
                     LVPLDGSPLAEAALAPAAALTAALAAPHRGALRLVQVVKAFQSKAEEGLVSELNEEAR
                     RLAHSYLATAAQRMESTVAGRALAVSFSVELASDVASTLLGVAEQEGPDRCDVIAIST
                     HGRGGLERWVMGSVTQRLLDTTRLPMLIVRPQREE"
     gene            1660..2550
                     /locus_tag="VGT44_09470"
     CDS             1660..2550
                     /locus_tag="VGT44_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV2581069.1"
                     /translation="MNLHQLRIFCSVVEQGSYTKAAETLYMTQPAISLQVRALERSLQ
                     VKLFERVNQQLAVTEAGQALYQCALPMLNAESEAERVLAELKGATRGKLVVAANTTGG
                     MYIIPPLLASYKERHPESELLLQIDATDRLIERARQGVIDLGFACGTISQPELTVETI
                     GTDILTLILSSRHPLASQPSLPLEQVVTLPFIVPESISRTRQFIEQAVRERGYSLKIV
                     MHFQGTEPVKKAVESNLGAAIVSRAAVAREVAAGVLCAIPIADLELTRPFVMFYRQGK
                     YFGPMAREFMVYMRQYALHK"
     gene            2708..4351
                     /locus_tag="VGT44_09475"
     CDS             2708..4351
                     /locus_tag="VGT44_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEV2581070.1"
                     /translation="MKYAFVIDQRKCIGCHACTVACKAEHDVPIGVYRTWVKYIEKGE
                     FPHARRYFLVNRCNHCDDAPCVAICPTKALFKRKDGIVDFDSSRCIGCKSCMQACPYD
                     ALYIDPNTHTAAKCNYCAHRTELGLEPACVVVCPERAIIAGDLHNSASEIARIIAREP
                     VRVRKPEQGTGPNVYYLGADEAAINPEAAVEELPHMTMWSTLLKPHHEDESAIDLGKS
                     LRPAHESNLSGQPRASNGSTPSQPASSHQGEARVVYDTPKNHAPWGWKVSTYLATKSI
                     GAGAMIVAALVLALNGWSNALASPLYALLGIGAPVIGGLFVAITLALLTADLKRPDRA
                     LYLVLKSNPTSWLVWGAYILGIFGLIQAAWFLFAVLGFTIGLYWLLVPAALFGIGAAG
                     YTALLFGQAEGRDFWQSPLLLPVLIVQAVLAGSSALGLLAWALNVGSAVTSMLTTVLF
                     VAIALHGLLALIEVFGPHTNSHVAAAAHYMTRGPLQQTFWGLFLAIGTVIPLLLLVIG
                     LFVPVAELALLGITGVLALVGLFAYEHCFVVAGQVVPLS"
BASE COUNT          881 a         1364 c         1261 g          882 t
ORIGIN      
        1 aatatcccga gcaacacgtt tgccaccgca agatagccca gaatggctga gagcggagag
       61 gtacctcctc gcacgacgag caggagcagg tatgacagtc cgccaagcaa caggctcgtc
      121 agcggcccga cgaaggacat ctggaactcc acgcccgggc tctttggctc ctgctcgatg
      181 ttggagaccc ctccgaagat gagcagggtg atgtttttca ccggcaagcc acgcgcgcgt
      241 gcgaccagcg agtgcgccag ttcatgcaga agcaccgagg cgaacagcag caaggtagcg
      301 agcaagctga cgaaccagta ggtgagcgtc gaccaacctg gatagagggc ggggaaccag
      361 ccggtcgcca gtgaccaggt gagcagcacc acaatcagga gccagctcac atggatagag
      421 atgtcaatgc cggcgatttt gccgagacgc aatgacccgg gcatggcgcg cctcctctca
      481 ttcttcgcgc tgcggacgca cgatcaacat gggcagcctg gtcgtatcga gcagacgctg
      541 cgtcacgctg cccatcaccc agcgctccag gcccccgcga ccgtgcgtgg agatggcgat
      601 cacgtcacac cggtcggggc cttcctgttc tgccacgccc agcagggtac ttgctacatc
      661 ggacgcgagt tcgaccgaga agctgacggc gagcgcgcgg cctgctaccg tcgactccat
      721 gcgctgcgct gccgttgcca ggtaggagtg cgccagccgg cgcgcctctt cgttcaactc
      781 gctgaccaag ccctcctccg cttttgattg aaaggctttg acgacctgca ccaggcgcaa
      841 ggcgccccgg tgcggagctg ccagggcggc ggtgagggcc gccgccgggg cgagcgcagc
      901 ttcagccagc ggcgagccat ccagcggaac gagggtgcgg atcgatcgcg ctgcctcgac
      961 gcgagctggc gtgccctcct ggcgcaggat gagtaccggc gcggcgctct ggtggacgag
     1021 cgtgtgagcc acgctgccca gcgcccaacg gctcaagccg gttctcccgt ggctgcagag
     1081 gacgacgagg tcggtcccgt gagcctcgat gctctcgatc aactgctggc ctggaaagcc
     1141 atacagcacc tcggtcatga tttcgattcc cgaaagggct ggccgcgcgg ccaccgcagt
     1201 gaggtagtcg gtggctccag ccatctccga ctccaggatc tgatcggtca tcagtggcac
     1261 aggggcaagc ccgccgctgt agtcgatggg cgggttgacc acccgtgcca gcagcagcct
     1321 accctttgcg gcgcgtgcga ggcgctcggc caccggcagc gcctgctccg cgcgagtaga
     1381 tccatcgagc ggaaccagta tattttgaaa catagcaacg ctcctttcca attcatattt
     1441 ttcctactga aagcgtaggc tttgcctgtc acaggagcct cacatatggg taggtcgcgc
     1501 tcacaaagca ctcacaagac gacttcttgc tcctgcttcc caaatgagaa ctataaatgg
     1561 gcgatgatcg tgtcatctcg gctataatga ggaggataat gagatagcct gccaggaggc
     1621 gcctatctgg cacatatgcc acttttgaaa gtaagagcaa tgaacctcca tcaactgcgt
     1681 attttttgta gtgtggtaga gcagggcagc tacaccaaag cagccgaaac gctctacatg
     1741 acccaaccgg cgatcagttt gcaggtacgc gccctggaac gcagcctgca ggtgaagttg
     1801 tttgagcgcg tgaaccagca attggcggtg accgaagcag ggcaggcgct ctatcagtgc
     1861 gcgcttccca tgctcaacgc ggaatcagag gcggagcgtg ttctggcgga actcaaggga
     1921 gcaacacgag ggaaactggt agtagcagcg aataccacgg gcggcatgta tatcattccg
     1981 ccactgcttg cttcgtataa agagcggcat cccgaaagtg agttgctctt gcaaatcgat
     2041 gcgaccgatc gcctgatcga gcgcgcgcgc cagggtgtga tcgaccttgg ctttgcgtgt
     2101 ggcacaatct ctcaacctga actaaccgtc gagaccattg gcaccgacat tctgacgctc
     2161 atcctctcct cgcgccatcc cctggcttcc cagccctcgc ttcccctgga acaggtcgtt
     2221 actttaccct ttattgttcc cgaaagcatc tcacgcaccc ggcaattcat cgaacaagca
     2281 gtacgcgagc gcggctatag cttgaaaatc gtgatgcact ttcaaggaac cgagccggtc
     2341 aaaaaggcgg tcgagtccaa cctgggggcc gctattgtct cgcgcgccgc ggtagcgcgg
     2401 gaggtggcag cgggcgtctt atgtgccatt cccatcgctg acctggaact cacacgcccc
     2461 ttcgtgatgt tctatcgcca ggggaaatat tttggtccga tggctcgcga atttatggtc
     2521 tacatgcgcc agtatgctct ccataaatga atctttatgg ttagaagtaa aaaaataaac
     2581 tagacaaacg atggtttctt ccctacacta gataacagag acgtaagcca gtccgagccg
     2641 cgtaagtttg cgtccagata acatatcgcg ccaggtgttc ctacagacga aagtgaggga
     2701 acctcccgtg aagtacgcct tcgtgatcga tcagcgcaag tgcattggct gtcatgcctg
     2761 tactgtcgcc tgtaaagctg aacacgatgt ccctatcgga gtctacagga catgggtgaa
     2821 gtatatcgaa aaaggcgagt tcccccatgc ccgtcgctat tttctggtga accgctgcaa
     2881 tcactgtgat gatgctccct gcgtggctat ttgccccaca aaagccctct ttaagcgcaa
     2941 ggatggcatc gtcgacttcg attccagccg ctgcatcggc tgtaaatcgt gcatgcaggc
     3001 ctgcccctat gacgcgctct acatcgaccc caacacgcac accgccgcca agtgcaacta
     3061 ctgcgcccac cgtaccgagc ttggtctgga acctgcctgc gtggtcgtct gccccgaacg
     3121 cgccatcatc gcgggcgacc tgcacaattc agccagcgaa atcgcccgca tcatcgcccg
     3181 cgaaccggtg cgcgtgcgca aaccagaaca gggcaccggc cccaacgtct actacctcgg
     3241 cgcggacgag gccgccatca atccggaggc cgccgtcgag gaacttccgc acatgacgat
     3301 gtggagtacg ctgctcaagc cacatcacga agacgaaagc gccatcgacc tgggcaaatc
     3361 gctgcgcccc gctcatgaaa gcaacctgtc ggggcagcct cgcgcctcca acggctccac
     3421 tccgtcgcaa ccggccagca gccaccaggg cgaagctcgc gtggtctatg acaccccgaa
     3481 gaatcacgcc ccctggggct ggaaagtctc gacctacctc gccacaaaat cgattggcgc
     3541 gggcgcgatg atcgtcgcgg cgctcgttct cgccctgaac ggctggagta acgcactcgc
     3601 ttcccctttg tatgcgctgc tgggcatcgg cgcgccggtg atcggcggcc tcttcgtcgc
     3661 catcaccctg gcgctgctca ccgccgacct caagcggcct gaccgcgcgc tgtatctcgt
     3721 gctgaagtcc aacccgacct cgtggctggt ctggggcgcc tacatcctgg gcatcttcgg
     3781 gttgatccag gcggcctggt ttctcttcgc tgtactgggc tttaccatcg gattgtactg
     3841 gctgctcgtt cctgccgcgc tcttcggcat aggcgcagca ggctataccg cgttgctctt
     3901 cggccaggcc gagggccgcg atttctggca gagtcccctc ctgctgccgg tgttgatcgt
     3961 ccaggcagta ctggccggtt cctcagcgct gggtctgctg gcctgggcgc tcaatgtggg
     4021 aagcgcagtc acttccatgc tcacgaccgt gctgttcgtc gcaatcgcgc tgcatggttt
     4081 gctcgctttg atagaagtat ttggcccgca cacgaacagc catgtcgcgg cagccgccca
     4141 ttacatgacc agaggcccgt tgcaacaaac cttctggggg ttgttccttg ccattggcac
     4201 agtaattcca ctgctcttgc tggtgattgg tttgttcgtg cctgtcgccg aactcgcact
     4261 cctcggcatc acgggtgttc ttgcgctggt tggtctcttt gcgtatgagc attgctttgt
     4321 cgtagctggt caggttgttc cgttgagttg atacaccaac gtccgtaggg gccattgtaa
     4381 gtcttcga
//
LOCUS       DASZAK010000538         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_189738, whole genome shotgun sequence.
ACCESSION   DASZAK010000538 DASZAK010000000
VERSION     DASZAK010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189738"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VGT44_09480"
     CDS             complement(<1..1520)
                     /locus_tag="VGT44_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581071.1"
                     /translation="MAAYVLAIIWLNAYIVRQVFFIEFMGKMNSMHGFWIAMARLAGA
                     HWYKPTWWPYWYIGMPFDYTYAPLVPALTAAIARMSGVSAAHGFQIVSGGVYCLGPLA
                     LFLMAWQVTRRAGWSFIAAVVYSLSAASELLLPDAKFDLVHLGDARRLYLIFVWDEVP
                     HQLGLAMVCLAVLFLARALRDDRFRSYVWAGLFVALALLASAFGATGLLLFAGCLLVT
                     CETSAWKRNFICVFLCGALGYLAMCPFLPPSLISVIRTNAGLFPNMAFTAASLGTLAA
                     VVSVGVLLWFVSRKWRPWYVRFFLLLTYVTLVIPALEEKLGLHFFPQAGRYKVELELT
                     LVLLAVFGAALVIDRRPKAARIVLAILLLWPAYTQVISHRRFSKNTIQAADVTETIEY
                     QVAKWVEPNLPGWRVLAPGSIAQWLNTFSKTPQFTGGSFPTAPNLVQLRAASDLVGMS
                     EAAIPAIWYKAYGVDAVVVAGQDSPEFWRPHPRGHQFDGVFPQLWEERDTRIYAVPR"
BASE COUNT          482 a          645 c          600 g          291 t
ORIGIN      
        1 cgcggcaccg cgtagatcct ggtgtcgcgc tcctcccaca gttgcggaaa cacgccgtca
       61 aactggtgcc cgcgcggatg cggtcgccag aactcgggac tgtcctggcc ggctacgact
      121 accgcgtcca ccccgtacgc cttgtaccag atcgctggaa tcgcagcttc ggacatcccc
      181 accaggtccg aagccgcccg cagttgcaca agattcggtg cggtcggaaa tgagccgccc
      241 gtgaattgcg gcgtcttgct gaacgtgttg agccactgcg caatcgatcc cggcgccagc
      301 acgcgccatc ccggcagatt cggctccacc cacttcgcca cctggtattc gatcgtctcc
      361 gtgacatcgg ccgcctggat cgtgttcttg ctgaagcgcc gatgcgaaat cacctgcgtg
      421 tacgccggcc acagcagcag gatcgccagc acgatccgcg cggctttcgg ccgccggtcg
      481 atcacgagcg cggccccgaa caccgccagt agcaccagcg tcagttccag ttccactttg
      541 tagcgcccgg cctgcggaaa aaaatgtagc cccagcttct cctccagcgc gggaatgaca
      601 agggtcacat aggtcagtaa caagaagaat cgcacatacc atggacgcca cttccgcgac
      661 acaaaccaga ggagcactcc taccgagacc accgcagcca gcgtccccaa tgacgccgcc
      721 gtgaatgcca tattcggaaa caggccggca ttcgtccgaa tcacggatat gagcgacggc
      781 ggcagaaacg ggcacatcgc caggtatccc agagcgccgc aaagaaacac acagatgaag
      841 ttgcgcttcc acgcgctcgt ttcgcaggtc acgagcaggc atcccgcaaa cagcagaagt
      901 ccggtcgcgc cgaaggcgct cgccagcagg gccagcgcga cgaacagtcc cgcccacaca
      961 tagctgcgga atcggtcgtc gcggagcgca cgcgccagaa acagaacggc caggcagacc
     1021 atcgccagcc cgagttggtg tggcacctcg tcccaaacga agatgaggta caaacgccgc
     1081 gcatccccta agtgaacaag gtcgaacttg gcgtccggca gcagcagctc cgaagcggcc
     1141 gacagcgagt agaccaccgc cgcgatgaaa ctccatcccg cccgccgggt cacctgccac
     1201 gccatcagga acaacgccag cgggccgaga caataaacgc ccccggacac gatctgaaac
     1261 ccgtgcgccg cagaaacacc ggacatccgc gcgatggcgg cggtgagcgc cggcaccagc
     1321 ggcgcatagg tgtagtcgaa aggcatgccg atgtaccagt aaggccacca tgtgggctta
     1381 taccagtgcg cgccggcaag ccgcgccatc gcgatccaga aaccgtgcat cgagttcatc
     1441 ttccccatga actcgatgaa aaacacctgc cgcacaatat aagcgttcag ccagatgatc
     1501 gccaggacat atgcggccac gagaagtttc tgctgaagtg agcgcgacaa gcaagcagaa
     1561 taacaaattg tggggcagcc cttctgggct ggatagcgtc gggataaacc ggcgtgaagt
     1621 agtgaatgaa agagtcacac aggaaaggag tagcgaacca tcctgacctc gacccatgcg
     1681 agggcgtcgc gaagccaagg gcgcacttga agcgttggga agaggcacat gcaggctggg
     1741 tatcccgaag ttgtgagctc cgaaatcggc aaattcaggg aagtcgacgg tgtcagcctg
     1801 accggaagac aacagtgcgg cgcgcggaaa cgcgagtgcg acgcagccct gcggagtcgt
     1861 agaccccagg catgcatgga aactgggtgc cctctgggca cgcgagaacc gagaggccct
     1921 gctgtcatcc gcagccgcaa gggggcggag cggtgagtga aagcgatgag cgataagact
     1981 cacatgaacg acagtgggga gtcgtacagc gccatagt
//
LOCUS       DASZAK010000539         1766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_511171, whole genome shotgun sequence.
ACCESSION   DASZAK010000539 DASZAK010000000
VERSION     DASZAK010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511171"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1438
                     /locus_tag="VGT44_09485"
     CDS             137..1438
                     /locus_tag="VGT44_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581072.1"
                     /translation="MHAMYEPSEPEYFLGSFPRDNFPVDAWTERPATLPVEAWTTETT
                     HRDGQQGGLPLSTETSLAIYDIHCRFTSDSGAIRQAEFFVYRPSDRAALQGALERYRG
                     GAPIEPTTWIRAAAKDVALIGSLGVRETGMLASASDYHTFHKFKPGGRNQAAQTYLDA
                     VKMTLDAGIRPRLHLEDATRAPMDFMRPFIENVQEMCATYGPDLRPKFRVCDTMGLGL
                     LYDDVALPRSIPRIFGEMRKLGLQPADLEFHPHNDTWLIVANCLAAIRSGCGVINGTS
                     LGKGERSGNAPLEAVLLHMIGMGYFHEKQPDFTALNELADLYANMGEAVPARYPLYGR
                     DAHRTRAGIHADGLNKFWWMYAPFNVPQLLGRPLEVSLTKESGLAGLIFVIRQHTGLD
                     LPKDDPRLQQTYAWLMEEFDNGRQTSVEWEELAPIFEGQPS"
     gene            complement(1516..>1766)
                     /locus_tag="VGT44_09490"
     CDS             complement(1516..>1766)
                     /locus_tag="VGT44_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV2581073.1"
                     /translation="LAVSAGYTRIEHRPSVQGAMYGVSLAVVGLLLTVVWTILHQPGV
                     DWRGLLIAAAAFGLALTRKVNLLLILALAGVVGFLLYR"
BASE COUNT          366 a          520 c          571 g          309 t
ORIGIN      
        1 tttatcaagc tgtcgggtac aactattagg agtagtagca aagtgaatag atggcgagcg
       61 tagcatggcg accgcgaggg tcgccatgct acaaacgatg aaccgcaccg taaaacatat
      121 tacaaggaga tcacctatgc acgcgatgta tgaaccgtcg gagccggaat actttcttgg
      181 gagttttcca cgcgataact ttccggtgga tgcgtggacg gagcgacctg ctacgctgcc
      241 ggtcgaggcg tggacgacgg agacgacgca tcgtgatggg cagcagggcg ggttgccgct
      301 ttcgaccgag acgagcctgg cgatttatga cattcactgc cgtttcacgt ccgattcggg
      361 ggcgatcagg caggccgagt tctttgtcta tcgcccatcc gaccgcgccg cgctgcaagg
      421 ggcgctggaa cggtatcggg gcggcgcgcc gattgagccg accacctgga ttcgcgcggc
      481 ggcgaaggat gtggcgctga ttggcagcct gggtgtgcgc gagacgggga tgctggcttc
      541 ggcttcggac tatcacacct tccacaaatt caagccgggc ggcagaaacc aggcggcgca
      601 gacctacctg gacgccgtga agatgacgct ggacgcgggt attcgtcccc gcctgcatct
      661 cgaagacgcc acgcgcgcgc cgatggactt catgcgccca tttatcgaaa acgtgcagga
      721 gatgtgcgcg acctatggcc ccgacttgcg cccgaaattt cgcgtttgcg atacgatggg
      781 actcggcctg ctctatgacg acgtggccct gccgcgcagc atcccgcgca tctttgggga
      841 gatgcgcaag ctggggctgc aacccgccga cctggagttc cacccgcaca acgacacgtg
      901 gctgatcgtg gcgaattgcc tggcggcgat ccgttccggc tgtggcgtca tcaacggcac
      961 ctcgctcggc aagggggaac gcagcggcaa cgcgccgctc gaagctgtgc tgctgcatat
     1021 gatcggcatg ggctacttcc acgaaaagca gcccgatttc accgctctga acgagctggc
     1081 cgatctctac gccaacatgg gcgaagccgt gccggccagg tatccgctgt atggacgcga
     1141 cgcgcatcgc acacgggcgg gtatccacgc cgacggcctg aacaaattct ggtggatgta
     1201 tgcgccgttc aatgtgccac aactgctcgg caggccgctg gaggtctcgc tgaccaaaga
     1261 gagtggtctg gccggcctga tcttcgtcat tcgccagcat accggcctcg atcttcccaa
     1321 agacgaccct cgcctgcaac agacctatgc ctggctcatg gaggaattcg acaacggtcg
     1381 ccagacaagc gtcgaatggg aagaactcgc ccccatcttt gaaggccaac cgtcgtaggg
     1441 acagggggag tggatcgtga cctccgacgc ggacaggcac aaggcccccc tgtgtcccta
     1501 cggaatgtta gcaggttagc gataaaggag aaatcctaca acccctgcca gggcgagaat
     1561 gagcaggagg ttgaccttgc gggtcagggc caggccgaag gcggcagcag cgatcagtaa
     1621 acccctccaa tcgacgccgg gttgatgcag gatcgtccag acgacggtga gcagcaggcc
     1681 gacgacggcg agcgagacgc cgtacatcgc gccctgcacg gaggggcggt gttcgatgcg
     1741 cgtgtagccg gcggagacgg cgagga
//
LOCUS       DASZAK010000540         5142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_688713, whole genome shotgun sequence.
ACCESSION   DASZAK010000540 DASZAK010000000
VERSION     DASZAK010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_688713"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..637)
                     /locus_tag="VGT44_09495"
     CDS             complement(101..637)
                     /locus_tag="VGT44_09495"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEV2581074.1"
                     /translation="MIDTTLISFIVLAVASVASALGVILFRNAVHSALSLILTLLFLA
                     LFYLQLGAMFIAIVQILIYAGAIMVLFLFVVTMLASEARDTEMPDRIPWQRGIAAGLG
                     LILVGALSYLLFTGASIDSSAKASGANSLLQAVATRGGDAQAFGYALFHGFAFPFEVT
                     SLLIVVAILGAMVLGRKS"
     gene            complement(634..1269)
                     /gene="nuoI"
                     /locus_tag="VGT44_09500"
     CDS             complement(634..1269)
                     /gene="nuoI"
                     /locus_tag="VGT44_09500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEV2581075.1"
                     /translation="MSFDIIKGMATTLKNIGKKPTTISYPEQERELPARFRGRHVLHR
                     YENGLERCVGCFLCAGACPADAIYIEADENSEEQRVSPGERYAKVFDVNLLRCIFCGY
                     CEAACPTGAITLEKQYKLAAYFPEDLVYHKEQLLEPVGQATVGSAADWFEQPPKEAAQ
                     PVPQDSHLFDGQQASAAAVGLGDLPTQQNVNITNEEQDEDRRKFEQEGEQR"
     gene            complement(1290..1631)
                     /locus_tag="VGT44_09505"
     CDS             complement(1290..1631)
                     /locus_tag="VGT44_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581076.1"
                     /translation="MTAYTYKKVLTDIVNQIQQLSPEEQERLLDDLAVIIRSTKIDQP
                     EMHSIKELRGLGKELWRGIDVEKYIEEERNSWTEESAHSLLDLEGLGAELWEGIDPQK
                     YVEEERKAWRK"
     gene            complement(1721..1945)
                     /locus_tag="VGT44_09510"
     CDS             complement(1721..1945)
                     /locus_tag="VGT44_09510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581077.1"
                     /translation="MPQLTYSEILGQIRYLTLEEQARLLEELAGLVHARIKALPKHSV
                     LEFKGIAGDFWQDVDIKQFIEEERNSWDRD"
     gene            complement(1983..3182)
                     /gene="nuoH"
                     /locus_tag="VGT44_09515"
     CDS             complement(1983..3182)
                     /gene="nuoH"
                     /locus_tag="VGT44_09515"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEV2581078.1"
                     /translation="MLEFLNTPIVAYIIKSAIVIFALLTAFAYMTLIERRVVAKLQGR
                     LGPNRAGPSGMFQPVADALKMAFKEQIVPTQAKKAIYLVAPIISVVVALCAFAVVPIG
                     RNWNPAQPSVWDPIIGDINVGLLWILSISSLAVYGIVLGGWASGNRYSLLGSLRSAAQ
                     MVSYETSLGLALSGVLMWAGTLSLVGIVQAQRNMGIWFIAAQPLGFFLYLIAGVAEVN
                     RAPFDLPEAEQELTAGYLTEYSGLRWSLYQMAEYINMITVSAVAATLFLGGWSFFGLE
                     NIPVLSIVIFLVKVAIFLFMFIWLRATLPRIRYDRLMRLGWQLLLPLAVLNAIITATV
                     VALGWPWWVSGLAGLVVIVAVLLFERSKNISVQQPMTAINDDGTTREVMLPTSVRLAK
                     FEKAGVE"
     gene            complement(3175..>5142)
                     /locus_tag="VGT44_09520"
     CDS             complement(3175..>5142)
                     /locus_tag="VGT44_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2581079.1"
                     /translation="KPPQTACTTPVSEGMIVYTKTERVLKARRGTLEFLLINHPLDCP
                     ICDKGGECDLQDFTLRHGPGGTRFDLYKRHYPKPVPVSDRVMLDRERCILCQRCTRFS
                     SEISMDNGLVMIARGYRMEVGTAPDHAFDSIFSGNTVEMCPVGALTASAYRFKSRPWE
                     LKRIPSVCNNCSVGCNARVDVRVDKVMRLMSRTNDAIDDGWLCDRGRWGFEFVNSPQR
                     LRAPLIRQKGTLQPATWDEAIYMIASRLRLIADKHGAQAIGGIGSTRTTNEEAYLFQK
                     LLREAIGTPNVDHYHGSFPGPRDTLTGQPWRMTNSIKDIEKAADIVLVASDPYERQPI
                     LNLRIKKAMKGGARIHIVNEGATELDRLAASRISIPQDGTGMAAKLLLRYALNDEAAQ
                     ADRHEELRAGLAQYDEALSAAEQSFGVEVVAQLQTLAHTIATSKGAILLYDEMATLAP
                     GSADLAADMQALAFATNNIERPSSGVGPLFEDANSLGARDMGLLPDALPGYQPAPQQG
                     LSYDEMLSSTEIRALYVMGANPLRHVAQLPPSLELLIAQDIAPTETVQRADIVLPAVS
                     FAEKDGTLTNVDAHVQRNVHALRPLPGAKADWEILTLLARQLGQKWNYASPQDVLLEI
                     AQANPLYAGLTWDELGAQGVRLQERGVVEHA"
BASE COUNT         1027 a         1580 c         1462 g         1073 t
ORIGIN      
        1 aagcgaaggg tctggctcaa tgggcggcga gatgcttcgc ttcgctcagc atgacatttc
       61 tgactctagt tgttaacatt catcaacggt gaaaggaagt tcaggatttt ctccccagca
      121 ccatcgcgcc caggatggcc accacgatca acaggctcgt gacctcgaag ggaaaggcga
      181 aaccgtgaaa gagcgcgtag ccaaacgcct gtgcgtcgcc cccgcgcgtg gcaacagcct
      241 gcaacaggct gttcgcgccg ctcgctttgg cgctgctatc gatggatgct ccggtgaaga
      301 gcaggtagct caacgcgccg accaggatca ggccaagccc ggcggcgatc ccgcgctgcc
      361 agggaatgcg gtcgggcatt tcagtatccc gcgcctcact tgccagcatg gtgacgacga
      421 agaggaatag caccataatc gccccggcgt agatcaggat ttgcacgatg gcgatgaaca
      481 tggcccccag ttgcaaataa aatagcgcca ggaacaacag cgtcaaaatc aggctcaacg
      541 cgctatgtac ggcgttgcga aaaagaatga cgcccagcgc cgaggccacg ctggcgacgg
      601 ccagcacaat gaaggatata agcgtggtgt cgatcatcgt tgctctccct cctgctcaaa
      661 cttgcgccgg tcctcgtcct gctcctcgtt ggtgatgttg acgttctgct gcgtcggcag
      721 atcgcccagc ccgacagcgg cggcgctggc ctgctgccca tcaaagaggt ggctatcctg
      781 cggcacaggc tgcgcggcct ctttcggcgg ctgctcaaac cagtccgctg cggagccaac
      841 ggtggcctgc ccgaccggct cgagcagttg ttccttgtga tacaccaggt cttcggggaa
      901 ataggccgcc agcttgtact gcttttcgag cgtgatcgcc cccgtcgggc aggccgcctc
      961 gcaatagccg cagaagatgc agcgcagcaa attcacgtcg aaaaccttcg cgtagcgttc
     1021 acccggcgag acgcgctgtt cctcgctatt ctcgtcggcc tcgatataga tagcatccgc
     1081 cgggcaagcc cccgcgcaca aaaagcatcc cacgcaccgc tccaacccgt tctcatagcg
     1141 atgcagcacg tgccgcccgc gaaaccgcgc cggcagctcc cgctcctgct ctggatagga
     1201 gatcgtagtg ggcttcttgc caatattctt cagggtggtg gccatccctt tgataatgtc
     1261 gaatgacata ttacttcgta ctccttagcc tatttgcgcc atgcttttct ttcttcttca
     1321 acatatttct gtggatctat gccttcccaa agttctgccc ccaacccttc cagatctaaa
     1381 agactatgcg cagactcttc tgtccatgag ttacgttctt cttcaatata tttctcaacg
     1441 tcaataccac gccatagctc tttacctaat cctctcaact ccttaatgct gtgcatctcg
     1501 ggttgatcta ttttagtgct acggataatt acagcaaggt catccaacaa gcgttcctgt
     1561 tcttcgggag aaagttgctg aatctgattg acaatatcag tcagcacctt tttgtaggtg
     1621 taggccgtca tgctttctat ccttagaact gatacgttta attgcaatca gatcatacca
     1681 gagctactgc gacttgtcat catcgttaca aactcaaatc tcaatctcga tcccaggagt
     1741 tccgctcctc ctcaataaat tgcttgatat ctacgtcttg ccagaaatca ccagcaatgc
     1801 ctttaaattc caaaacacta tgtttaggca atgcttttat gcgggcatga accaatcctg
     1861 ccaactcttc tagaagtcgt gcctgttctt caagcgtaag ataccggatt tggccgagta
     1921 tctcgctgta agttagttgt ggcatcggta tactccagat caatgagcca ggacgggcgc
     1981 aatcattcca ctcccgcctt ctcgaacttc gccaggcgca ccgacgtggg cagcatgacc
     2041 tcccttgtcg ttccatcatc atttatggct gtcatgggct gctgtacgct gatgttcttg
     2101 ctgcgctcaa agagcagcac ggcaacgatc accaccagcc ctgccagccc gctgacccac
     2161 cagggccagc ccagcgccac aacagtagcg gtgatgatag cgttcagcac agccaggggc
     2221 agcagtagtt gccagcccag gcgcatcagg cggtcgtagc ggatgcgcgg cagtgtggcg
     2281 cgcagccaga tgaacatgaa caggaagatg gccactttga ccaggaagat cacgatggaa
     2341 agcaccggaa tattttccag gccgaaaaag ctccaaccgc ccaaaaagag cgtcgccgcc
     2401 accgcgctga ctgttatcat gttgatgtat tccgccatct ggtagaggct ccagcgcaga
     2461 ccgctgtact cggtcaggta gccggcggtc agttcctgct cggcttccgg cagatcgaaa
     2521 ggcgcgcgat taacctcggc cacgccggcg atcaggtaca ggaagaagcc caggggctgc
     2581 gcggcgatga accagatgcc catgttgcgc tgcgcctgca caatgccgac caggctgagc
     2641 gtaccggccc acatcagcac gccgctcaac gccagtccca ggctcgtttc gtaggagacc
     2701 atctgcgccg cgctgcgcag cgaacccagc agggagtagc gattacccga tgcccagccg
     2761 cccagtacta tgccgtacac cgccagcgac gagatcgaga gaatccatag caggccaaca
     2821 ttgatgtcgc cgatgatggg gtcccagacg ctcggctgtg ccggattcca gttcctgccg
     2881 attggcacga cggcgaaggc gcacagcgcc acgacgaccg aaatgatcgg cgcaaccagg
     2941 tagatggctt ttttcgcctg cgtcggcacg atttgttcct tgaaggccat cttgagcgcg
     3001 tcggcgacgg gttggaacat gccgctcggc ccggcgcggt tcggccccag gcggccttgc
     3061 agcttcgcca ccacgcgccg ttcgatcagt gtcatgtagg caaaggcggt cagcagcgcg
     3121 aatatcacga tggcgctttt gatgatgtac gccacgatgg gcgtattcag aaactcaagc
     3181 atgttccacg accccccttt cctgcaagcg cacaccctgc gcgcccagtt cgtcccacgt
     3241 cagcccggcg tagagcgggt tcgcctgcgc gatttcgagc agcacatcct gcggactagc
     3301 gtagttccac ttctggccaa gctggcgcgc cagcagcgtc aggatttccc agtccgcctt
     3361 cgcgccggga agcgggcgca gcgcgtggac gttgcgctgc acgtgcgcgt caacgttggt
     3421 cagtgtgcca tccttctcgg cgaagctgac tgccggcagc acgatatcgg cgcgctggac
     3481 ggtctccgtc ggggcgatat cctgcgcgat cagcaattcc aggctgggcg gcaactgcgc
     3541 aacatggcgc aggggattcg ctcccataac gtagagcgcg cgtatctccg tgctgctcaa
     3601 catttcgtca taagagagtc cctgctgcgg agcgggttga tagcccggca acgcatccgg
     3661 cagcagaccc atgtcgcgcg cgccgagcga gttggcgtcc tcgaaaagcg gcccgacgcc
     3721 cgaactgggt cgctcgatgt tgttggtcgc gaatgccagc gcctgcatat ccgccgccag
     3781 gtcggcgctg ccgggtgcca gcgtcgccat ctcgtcgtag agcaggatcg cgcccttcga
     3841 cgtggcgatg gtatgcgcaa gtgtttgcag ttgtgcaacg acctcgaccc caaaggactg
     3901 ttctgcagcg ctcaacgcct cgtcgtactg tgctagcccg gcgcgaagct cttcatgccg
     3961 atcagcctgc gctgcctcgt cgttcagcgc gtagcgcagc agcagctttg ccgccatgcc
     4021 cgtaccgtcc tgcgggatgc tgattctgct ggcggccagc cggtcaagct cggtcgcgcc
     4081 ctcgttgacg atgtggatac gcgcgccgcc cttcatagcc tttttgatgc gcaggttgag
     4141 aataggctgg cgttcgtagg gatcagaggc caccagcaca atgtcggcgg ccttctcgat
     4201 atccttgatg ctgttcgtca tgcgccaggg ctgaccggtc agtgtatcgc gcgggccggg
     4261 gaaggaaccg tgatagtgat cgacgttggg cgtaccgatg gcctcgcgca gcagtttctg
     4321 gaagagatag gcttcctcgt tggttgtgcg cgttgagccg atgccgccga tggcctgcgc
     4381 gccgtgcttg tcggcgatca ggcgcaggcg cgaggcgatc atatagattg cctcgtccca
     4441 ggtagcaggt tgcaatgttc ccttctggcg gatgagcggt gcgcgcagtc gctgcgggct
     4501 gttgacgaac tcgaagcccc agcgcccccg gtcgcacagc cagccatcgt cgatcgcgtc
     4561 gttcgtgcgc gacatcaggc gcatcacttt gtccacgcgc acatccacgc gcgcgttgca
     4621 gccgacgctg caattgttgc acacgctcgg aatgcgtttg agttcccagg gcctcgattt
     4681 gaaacgataa gccgaggccg tcagcgcgcc gacgggacac atttcaaccg tattgccgga
     4741 gaagatcgag tcaaaggcat gatcgggtgc ggtgccgact tccatacgat agccgcgcgc
     4801 gatcatcacc aacccgttat ccattgagat ttccgagctg aagcgcgtgc agcgctggca
     4861 caggatgcag cgctcgcgat ccagcatgac gcggtcgctg acaggcaccg gcttgggata
     4921 gtgacgcttg taaagatcaa atcgcgtccc gcctggccca tgccgcagcg tgaagtcctg
     4981 caggtcgcac tcgccgccct tgtcgcagat aggacaatcg agcgggtggt tgatgagcag
     5041 gaattcgagt gtgccgcgcc tggctttgag taccctttcg gttttcgtat agacaatcat
     5101 tccctcgctg acgggcgtgg tgcaggccgt ctgcggcggc tt
//
LOCUS       DASZAK010000541         3476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_618263, whole genome shotgun sequence.
ACCESSION   DASZAK010000541 DASZAK010000000
VERSION     DASZAK010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3476
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_618263"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="VGT44_09525"
     CDS             <1..1784
                     /locus_tag="VGT44_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV2581080.1"
                     /translation="RVIAPNVGGGFGGKSDPFSHEICAAELSRRTGRPVKITCTREEV
                     FLIHRGRHPVKMWIKTGVKRDGTLTAMHFRSFLDGGAYGSYGIATTYYTGALQTVTYK
                     LPCYKFEGMRLFTNKPPCGPKRGHGTTQPRYAVEVHLDKLACDLGIDPVELRRHNLIE
                     PYSHTVNGLRITSCALGECLDAVIARSGWNEFHTQHPVGADLSRPSPIYRPSEIANTP
                     ESSIKRGMGIAASSYICGAGKPIYWNDLPHSAVQIRLDRGGGVTIYCGATDIGQGSTS
                     VLAYIVAEELGVPPEHIHLETADTTLTPVDLGSYSSRVTFMAGNAAIAVARKLKGQLF
                     ELASEQLHVPIENLEAADCLIYDENDPGMALPFVKVVQLTEARYGALVAAGSYAPPEG
                     LHGDYKGAGVGPSPAYSYSTCVAQVAVDVETGDLVVEKLWLAHDVGQSINPLLVAGQV
                     EGGAYMGYGEAVMEQQVFRKGLHKIPSLLDYKLPTTLDTPEIETILIEIPDREGPFGG
                     KEAGQGPLNPVIPAIANAVFDAVGVRIDETPITPDKILKALRAEAPGARPALRPHVEP
                     TAAPSPWPTRLIAWEPVEASLAAKDGV"
     gene            1784..2764
                     /locus_tag="VGT44_09530"
     CDS             1784..2764
                     /locus_tag="VGT44_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV2581081.1"
                     /translation="MLRLPPFRYLAPRTMEQATRLLAQEGEQAMLVAGGTDLYPNMKR
                     RQFTPPVLIGLRGLEALKRISGSPEQGMRLGAGVTLTTLAEHPAVLQHYPALATAAGS
                     VSTPQLRNMGTIGGNLLLDTRCNYYNQTEFWRHSINYCMKKDGDVCLVAPGSPRCWAI
                     SSADTAPVLVSLDAQVRLVSVRGERVLPVRELFRDDGINPYTREADEILSEIILPPAD
                     GWRSVYLKLRRRGSFDFPILGVAVALRFAADQTIAGARITLGAVASHPVEATDAATLL
                     TGQRLTPEIITEVATLAAKRSKPLDNADLTINYRKQVTPVYIRRALTSFL"
     gene            2781..>3476
                     /locus_tag="VGT44_09535"
     CDS             2781..>3476
                     /locus_tag="VGT44_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase family protein"
                     /protein_id="HEV2581082.1"
                     /translation="MSQLENASRSLIEEHQLARLQLGLARILPHNSFYEEKLLSGRAS
                     LPLWSLGDFAHFPFTTKQELAVDQELHPLYGSNLTYPLGDHVRLHQTSGTTGRPMKIL
                     DTQESWDWWADCWTSVYRAAGVTRDDIVFLAFGFGPFIGFWSAYEGAKRLGALTVPGG
                     GMDSVQRLRAMQEIGATVLVCTPSYARHLAEVAQQHGLDTRFSSVRVTIHAGEPGASI
                     PATRERIQSAWNAL"
BASE COUNT          742 a          987 c          955 g          792 t
ORIGIN      
        1 tacgagtcat tgcgcccaat gtcggtggag gcttcggtgg caagagcgat cccttttcac
       61 acgaaatatg cgccgctgaa ttgtcgcgtc gtaccggcag acctgtcaaa atcacctgta
      121 cccgcgaaga agtgtttttg attcacaggg gacgtcaccc tgtcaaaatg tggatcaaga
      181 ccggcgtaaa acgagacggc acgctgacgg ctatgcactt ccgttctttc ctcgatggtg
      241 gtgcgtatgg ctcgtatggc atcgccacga cctattacac gggcgcgttg cagaccgtga
      301 catataagct tccctgctac aaattcgagg gcatgcgcct ctttaccaac aagccgcctt
      361 gtgggcctaa gcgcgggcat ggcacgacgc agcctcgcta tgctgtagag gtgcatctcg
      421 acaaattagc ctgtgatctc gggatcgatc ccgttgagct gcgtcgccat aacctgattg
      481 aaccatactc ccatacggtc aatggtttgc gtatcaccag ttgcgctctg ggtgagtgcc
      541 tcgacgccgt aatcgcacgc tccggctgga acgaatttca tacccaacac cctgtagggg
      601 ccgatttatc gcgcccatcg ccgatttatc ggccctcgga aatagcaaat actcccgaat
      661 catccatcaa acgtggtatg ggcatagccg cgtcctcgta catctgtggc gccggtaaac
      721 ccatttattg gaacgatctg ccgcactccg ccgtacagat tcgcctcgac cgtgggggag
      781 gcgtcactat ctattgtggc gcgaccgaca ttggccaggg atcgacttcc gtgcttgcct
      841 acatagtcgc ggaagaactg ggagtacccc ctgaacacat tcacctggag accgccgata
      901 ccacgttgac gccggtcgat ctaggctcat attccagtcg agtcaccttc atggccggga
      961 atgctgccat cgccgtggca cgtaagttga aaggacaact cttcgagctc gcttccgaac
     1021 aattacatgt acccattgag aatctcgagg ctgcggactg cctgatctat gacgaaaacg
     1081 acccgggcat ggcgctgccc tttgtaaaag tagtgcagtt gaccgaggct cgctacggag
     1141 cgctggtggc tgccggttcg tacgctccac ctgaaggcct ccacggtgat tataaaggag
     1201 caggtgtcgg accttccccc gcctactctt actctacatg tgtagcacag gtggcagtcg
     1261 acgtggaaac gggcgatctg gttgtagaaa aactgtggct ggctcacgat gtaggtcagt
     1321 ctatcaaccc gctgttagtc gcgggccagg tcgagggggg cgcgtacatg ggctatggtg
     1381 aagcggtgat ggagcaacag gttttccgca aaggactcca taaaataccg tcgctgctgg
     1441 attataagct ccccacaacc ctggatacac ccgaaatcga gaccatcctg atcgagattc
     1501 ctgatcgtga gggtcccttt ggcggtaaag aggcaggtca aggcccgctt aatccggtta
     1561 ttcctgcgat tgccaatgct gtttttgatg cagtgggtgt gcgtattgat gagacgccga
     1621 ttacacctga caaaatactc aaggctttgc gagccgaagc gccaggcgct cgacctgcac
     1681 tgcgtccgca tgttgaacca accgcggcgc cgtctccctg gcccacgcgc ctgattgcgt
     1741 gggaaccggt ggaagcgtct cttgctgcta aggatggtgt ttgatgctgc gactcccacc
     1801 gtttcgctac ctggctcctc gcacgatgga gcaagccacg cggttgctgg ctcaggaggg
     1861 agagcaggct atgctggtcg cgggcggcac cgatctgtac cccaatatga aacgccgcca
     1921 gttcacaccg ccagttttga tcggtctgcg cggccttgaa gcattgaagc gcataagcgg
     1981 ctcgcccgaa cagggtatgc gactgggagc aggtgtaaca ctcactacac tggctgagca
     2041 ccctgccgtt cttcagcact atcccgcgct tgccactgcc gctggctctg tttcaacgcc
     2101 tcaactgcgc aacatgggca cgattggcgg taatctgttg ttggatacac gctgtaatta
     2161 ttataatcag accgaatttt ggcgtcactc gattaactat tgcatgaaga aggacggcga
     2221 tgtgtgcctg gtcgcgccgg gtagcccacg ctgctgggct atctcttcag ccgatacggc
     2281 tcctgtgctg gtcagcctgg atgcccaggt gcgcctggta agcgtgcgtg gcgaacgtgt
     2341 gttaccagta cgcgaactgt tccgcgatga tggcatcaat ccctacacca gagaggcgga
     2401 tgagatactc agtgaaatta tactgccacc cgcggatggc tggcgcagcg tctacctgaa
     2461 acttcgccgt cgtggttcat tcgattttcc tatacttggc gtcgcagtgg cgctccgctt
     2521 tgctgctgat cagacaattg ccggcgcgcg tattacgctg ggcgcagttg cctcccatcc
     2581 ggttgaagcc acagatgcag caacattatt gactggccag cgtctcacgc ctgaaattat
     2641 taccgaagta gctacactcg ctgccaaacg ctccaaaccc ttagataatg cagacctgac
     2701 aattaactac cgcaaacagg tcacacccgt ctatatccgc cgcgcgctaa cttcatttct
     2761 ttaggaagga acgtcactcc atgtctcaat tggaaaacgc ttcccgctcg ctcatagagg
     2821 agcatcaact ggcacggtta caactagggc tggcacgcat actccctcat aatagctttt
     2881 acgaagaaaa attgctctcc gggagagcat ctctcccatt gtggagctta ggtgattttg
     2941 cgcatttccc tttcacaacc aagcaagagc tggctgttga ccaggagttg catcccctct
     3001 atggcagcaa tctaacctac cctctaggcg accatgtacg cctgcaccag acttctggaa
     3061 ccaccggacg gcccatgaag attctggata cgcaggaaag ttgggactgg tgggccgatt
     3121 gctggacaag tgtctaccgg gcagctggtg tcactcgtga cgatatcgtc tttctggctt
     3181 tcggtttcgg cccatttatc ggcttctggt cggcttatga aggagccaaa cgccttggcg
     3241 cgctcacggt tccaggtggt ggcatggata gcgtgcagcg tctgcgggca atgcaggaga
     3301 ttggcgcaac cgtcctggta tgtacgccaa gctacgcgcg gcatttagcg gaggtagccc
     3361 agcagcatgg gctggatacg cgattttcga gtgtgcgtgt aaccattcat gccggtgagc
     3421 ccggcgcgtc gattcccgcc acgagagaac gcattcaaag cgcctggaat gcgctt
//
LOCUS       DASZAK010000542         1540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_511209, whole genome shotgun sequence.
ACCESSION   DASZAK010000542 DASZAK010000000
VERSION     DASZAK010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511209"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..>1540)
                     /locus_tag="VGT44_09540"
     CDS             complement(265..>1540)
                     /locus_tag="VGT44_09540"
                     /inference="COORDINATES: protein motif:HMM:NF020672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEV2581083.1"
                     /translation="LQTHRHSVPVAHPGSRVALNLANVARTDLQRGDVVALPGQFQQT
                     MLIDARLSVVRDATIALAHNALVDFYTGSQEVPASVRLLDANEVQPGQSAWAQLRLAR
                     PAVMSRRDRFILRIPSPSMTIGGGEVIDAHPRFHRRYQAPVMQSLETLERGSPDELVL
                     AALDRRKGGDVKRAQHGLAGYELADISKQSNLARDVTQNTLETLLLEGRVRRLGTLWF
                     AQAVWDVLRDETRRLLAEQHRQYPLRSGLSKEEWRSRLGLSPKMAAEVLAALQEEGQI
                     AEALGVTGGAGGYIRLPDFQPTFTPAQQQQVERLLAQFREHPFLPPGKAEAEAQAGSE
                     TLGALIEQGVLVKVGGASDAVLFLRENYDQALAALLAYIHEHGSITAAEARDILGTTR
                     KYILPLLEHMDERRLTRRVGDERVLGPTVPGS"
BASE COUNT          291 a          489 c          425 g          335 t
ORIGIN      
        1 cgatagatcg tcgccattcc gccgcgcccg atatgatcct gtaactggta gcgatctccc
       61 agaatttcag cctgcatagc tctccctggg tctgaaggga acgtccccat tcacaaacaa
      121 tgcgcctgtt gcatgtgtca gacgcgcgat aataccgtta acgtatgcct gtacgcgctc
      181 actattgtaa cgcattctgc tacttaatgg aacaccctgt actactaaaa taccaatatc
      241 ttgagtaatt catgagattt ttatttatga tccaggcacg gtgggcccca ggacgcgctc
      301 atctccaact ctccgtgtaa gacggcgctc atccatgtgt tcgagcaaag gcaagatgta
      361 cttgcgcgtc gttcccagga tatcgcgtgc ctcggcagcc gttatcgagc catgttcgtg
      421 tatgtaggct agcagcgctg ccagggcctg atcgtaattc tcgcgcagga aaaggactgc
      481 atcagacgcg ccgcctactt ttaccagtac tccctgctca atcagcgctc ccagtgtctc
      541 gctgccagcc tgcgcctctg cctccgcttt accgggtggg agaaagggat gctcgcgaaa
      601 ttgcgcaagc agccgttcga cttgctgttg ctgtgcggga gtgaaggttg gctggaaatc
      661 gggaaggcga atgtagccac cggcgccgcc tgtgacccca agcgcttctg ctatctggcc
      721 ctcttcctgc aacgccgcca acacctccgc tgccatcttt ggagatagcc ccaggcgcga
      781 acgccattct tctttggaga gtccactgcg cagtggatac tggcgatgtt gctcagcgag
      841 caaacgtcgc gtctcgtcgc gcaatacgtc ccataccgcc tgcgcgaacc agagcgtacc
      901 cagacgccga actcttcctt caagtaacaa cgtctccaac gtgttttgcg tcacatctcg
      961 cgccagattg ctttgtttcg agatgtccgc caattcatat cccgccagcc cgtgctgcgc
     1021 tctcttcaca tcgcctcctt tgcgccggtc gagcgcggcc agcacgagtt cgtccggcga
     1081 gccgcgttcc agcgtctcca ggctttgcat caccggcgct tgatagcgcc ggtggaagcg
     1141 tggatgcgcg tcgatgactt cgccgccgcc gattgtcata ctaggcgagg ggatacgcag
     1201 gataaaacgg tcgcgtctcg acatgactgc cggtcgcgcc aggcgcaact gcgcccacgc
     1261 gctctgccct ggctgcactt cattggcatc cagcaggcgc acgctggcgg gaacttcctg
     1321 cgagccggtg tagaaatcga ccagggcatt gtgggcgagc gctatggtcg cgtcgcgcac
     1381 gaccgaaagg cgcgcgtcga tcaacatggt ttgctggaac tgccccggca gcgccaccac
     1441 gtctccgcgc tgcaggtcgg tgcgcgccac gtttgccagg ttcaaagcca cccggctgcc
     1501 aggatgcgcc accggcacgc tgtgccggtg ggtttgcagc
//
LOCUS       DASZAK010000543         4857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_296780, whole genome shotgun sequence.
ACCESSION   DASZAK010000543 DASZAK010000000
VERSION     DASZAK010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296780"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VGT44_09545"
     CDS             complement(<1..1145)
                     /locus_tag="VGT44_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEV2581084.1"
                     /translation="MPNITILGAGSAMFARQLITDILQIEGLDEGNFALVDIDANRLE
                     LAHQLTERLISHSGKRWNVRASTERRDLLAESDFIINSIEVAGLANVRHDFDIPMKYG
                     VNQCIGDTIGPGGIFKALRTGPAWLDILHDAEQLCPRAWVLNYTNPMSILTLAALAGT
                     GMRTVGLCHSVQGTSRQLAGYLNIPYDELEWRCAGINHNAWFTKLRYQGQDMYPRLRE
                     LAQVSDIYERDPIRFEIMRYFGAFVTESSGHFSEYVPYFRKRPDLIERYCRSGYLGET
                     GFYANNWPTWRAENDASIREMLAGTRALPFTRSHEYGSSIIEAVTLRHPTVIYGNVRN
                     TGLIDNLPDGCVEVACLVDQNGVQPCHFGSLPEQLAGLNRAHMTVHT"
     gene            1339..2406
                     /locus_tag="VGT44_09550"
     CDS             1339..2406
                     /locus_tag="VGT44_09550"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV2581085.1"
                     /translation="MRLTRAVEDRTRTLFLQGKVVGGVYTAQGHEATTVGAAWTLGDG
                     DVIVPQHRDLGMHLTRGGSPRAVMCQWLARGNSPSLGRDGQLHIGDIHHGIVPSISML
                     GESLPVACGVALTMKMRRHSTIVMAPCGDGATSTGPFHEALNFAAVQQLPIVFVIENN
                     GYAYSTPTYKEFAIENLSERANAYGMRGESVDGNDVLKVIETVGRAVERVRSGKGPVL
                     VECKTFRVRGHSEADRADYVPPELREEWLAKDPIPRFEDYLTREKMLTPAKKAEIEVQ
                     VKAVVEDAVSFAEQGSAADPATVADYVFAPDGPIAIIGEPGAEDPRYINALDHRTGKP
                     FTTVTGTGTPEFVKTEEVGRR"
     gene            2403..3386
                     /locus_tag="VGT44_09555"
     CDS             2403..3386
                     /locus_tag="VGT44_09555"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV2581086.1"
                     /translation="MSAVTYLEAIGQAMREEMQRDEAVFLLGEDVGVYGGAFKVSAGL
                     LEEFGPERVIDTPMAESAIIGSAVGAALMGMRPIAEMQFIDFITCGFDQIINMANKMY
                     WRSGMPVPMVIRGPSGGGVKGGPFHSASPEAWFFHAPGIKVVVPSTTYDAKGLLKAAI
                     RDNNPVLYLEHKLLYRMPELREDLPSGDYIVPIGKAITRREGEDMTILTYGAMVHQCL
                     KAAVTLEQEDDLQVEVLDLRSLAPLDRDAILESVKRTNKALIVHEDTLTGGIGAELAA
                     ILAEDLFEYLDGPITRVAAPDVPFPYVPQLEAAFLPNAEKIVAAARKLAAY"
     gene            3420..4790
                     /locus_tag="VGT44_09560"
     CDS             3420..4790
                     /locus_tag="VGT44_09560"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEV2581087.1"
                     /translation="MPTPVKMPRLGESVAEGTLGSWLKNEGDWVERDESLAEIITDKI
                     NAELPSPVAGRLTKILVKVDETVPVGADIALIEENADVSASPPAEPAPGPDAAPIESP
                     DRANVAVMENREASDGNGSRAAAQASRVGAEERQRISPLARRLAREHDLDLNAIAGTG
                     TGGRVRKEDILAYVAQRQVAPAAAVAQPVVPAPAAQPASIPPAPVPVGTQFTATPAPV
                     MVGEEEVELITPSRMRLAIAEHMVRSKRTSPPATTVVEVDMTNIAKWLEKNKEDFKKR
                     EGYSISYVPFVMKAVCEGIRKFPLMNSSWTEDNKIIVKKRINLGMAVATDEGLVVPTL
                     YDTDQYTIAGLARQISAVAQRARANKLTIQDLQGSTFVVNNPGTFGTIISVPIINQPN
                     AGILSMDAVVKRPVVIEDDAIAVRSMMYLSLSFDHRILDGAGAGGFLQAVRTKLQSYG
                     REIDVY"
BASE COUNT         1104 a         1336 c         1430 g          987 t
ORIGIN      
        1 aaggtatgaa cggtcatgtg ggcgcgattc aatccggcca gttgttcagg cagggaacca
       61 aagtggcagg gctgcacgcc gttctgatcg acgaggcaag cgacctcaac gcagccatcg
      121 ggcaggttat cgatcagtcc ggtgttgcgc acgttgccgt agatcaccgt tggatggcgc
      181 agtgttactg cttcgatgat ggaggagccg tattcatggc tgcgggtgaa agggagcgct
      241 cgagtacccg ccagcatttc gcgaatggag gcatcgttct ctgcacgcca ggtgggccag
      301 ttattcgcgt aaaatcccgt ctcgcccagg tagcccgacc ggcagtaacg ctcgatcaag
      361 tcaggccgtt tgcggaagta ggggacatac tctgaaaagt gaccgctgct ctccgtgaca
      421 aaggccccga agtagcgcat gatctcaaag cgaatggggt cgcgttcata gatatcggat
      481 acctgggcca gttcgcgcag acgagggtac atgtcctgac cctggtagcg caacttcgta
      541 aaccaggcat tgtggttaat acccgcacag cgccactcca gctcatcata ggggatgttg
      601 aggtagcctg ccaactgcct cgaggtcccc tgcacgctat ggcacaggcc aaccgtgcgc
      661 atgccagtac cggcaagtgc cgcgagggta agtatcgaca tgggattggt gtagttgaga
      721 acccaggcgc gcgggcagag ctgttcagcg tcatgcagga tgtcgagcca ggcgggtccg
      781 gtgcgcagcg ccttgaagat gcctcccggg ccgatggtgt caccaataca ttgattgact
      841 ccatacttca ttggaatatc gaagtcgtgg cgcacattgg cgaggccggc gacctcaatg
      901 gagttgatga tgaaatcgct ctccgccaat aggtcgcgtc gctcagtgga ggcccgcaca
      961 ttccaacgtt tgccactatg gctaatcagt cgttcagtga gctggtgcgc tagctccagc
     1021 cgattcgcat cgatgtccac gagcgcaaaa ttaccctcgt caagtccctc gatctgcaag
     1081 atatcagtga tgagctgacg ggcgaacatg gcgcttccgg cacctaaaat ggtgatattt
     1141 ggcatggtca atccaactcc ctgggcgcag cctccaaagc acctttctta atgcagtata
     1201 tcatgtctgg gacgatttcc ttcttgacac cttttctccc cgcgtgctag catgaacgag
     1261 tgatacctct ctatttgagc aaaggaagtg atacttatgc gactggagcg ggctgacctg
     1321 ctgcagatat actatttaat gagactcacc agggccgtgg aagatcgtac ccgcacgctt
     1381 tttttacagg gtaaggttgt gggcggtgtc tacacggcgc agggacacga ggccacgact
     1441 gtcggggctg catggacgct gggcgatggc gacgtgatcg tgccgcagca ccgcgacctt
     1501 ggcatgcatc tcacgcgcgg cggctcgcca cgcgcagtga tgtgtcaatg gttggctcgc
     1561 ggtaattcgc cttccctcgg acgtgatggc caattgcata tcggcgatat acatcatggc
     1621 attgttccca gtatcagtat gcttggcgaa tcgctgccgg tggcgtgtgg cgtagctctc
     1681 accatgaaga tgcgcaggca ttcgaccatc gtcatggctc cctgtggcga tggcgcaacc
     1741 agcacaggcc cattccacga agctctaaac tttgccgctg tacagcaatt gcccatcgtc
     1801 ttcgtcatcg agaataacgg ttacgcctat tcaacgccaa catacaaaga gtttgcgatc
     1861 gaaaacctct ctgaacgtgc caacgcttat gggatgcggg gagagagcgt tgatggcaac
     1921 gatgtgctga aagtgatcga aacggttggt cgagcagttg agcgcgtgcg cagcggtaaa
     1981 gggcctgtac tggtagagtg caaaaccttc cgcgtgcgcg gtcactcgga ggcggacagg
     2041 gccgattatg tgcctcccga gctgcgagaa gaatggttgg cgaaggaccc gattccgcgc
     2101 tttgaagact acctgaccag ggaaaagatg ctaacaccgg caaaaaaagc tgaaatagaa
     2161 gtacaagtta aggcggtggt agaagacgcg gtgagtttcg ctgaacaagg gtccgccgca
     2221 gaccctgcta cagtggccga ctatgtgttt gccccggatg gcccgattgc aattattggc
     2281 gagcctggag cggaagaccc gcgctacatc aacgcgctgg accatcgtac tggcaagcct
     2341 ttcacgaccg tgaccgggac tggaacaccc gaattcgtta agactgagga ggttggtagg
     2401 cgatgagcgc ggtgacctat ttagaagcga ttggccaggc catgcgcgaa gaaatgcagc
     2461 gtgacgaggc cgttttcctg cttggtgaag atgtaggcgt ctacggcggc gcgttcaaag
     2521 tcagcgcggg tctgctggaa gagtttggac cagagcgagt gatcgataca ccgatggctg
     2581 aatccgctat catcggctct gccgtgggcg ccgcgctaat gggcatgcgt cccatcgccg
     2641 agatgcagtt catcgacttc atcacctgcg gcttcgatca gatcatcaac atggcgaata
     2701 agatgtactg gcgctccggc atgcccgtgc cgatggtcat tcgcggccct tccggcggtg
     2761 gtgtcaaggg tggcccgttc cattccgcca gcccggaagc ctggttcttc cacgcaccgg
     2821 gcatcaaggt tgtcgttcct tcgaccacgt atgatgcaaa agggctgctc aaggctgcca
     2881 tccgcgacaa caatcccgtg ctctacctcg aacataaatt gctctatcgt atgccggagc
     2941 tgcgcgagga tcttccaagt ggggactata ttgtgcccat aggcaaggct atcactcgtc
     3001 gtgaagggga ggatatgact atcctgacct acggggcgat ggtacaccag tgcttaaaag
     3061 ccgcagttac gctggagcaa gaggatgacc tgcaagtcga ggtgctcgac ctgcgcagcc
     3121 tcgcgccact tgaccgcgat gcgatcctcg agtcggtcaa gcgcacaaac aaggcgctga
     3181 ttgtacacga ggacacgctc accggaggca tcggcgccga actggcggca atactggccg
     3241 aagacctgtt cgagtatctc gatgggccga tcacgcgtgt ggcggcgccc gacgtgccat
     3301 ttccctatgt gccgcagttg gaggccgcct ttttgcccaa cgcggagaag atagtagctg
     3361 ctgcacgcaa gcttgcagcg tattaaacga attgcaactg acaaaggagt cacaaacata
     3421 tgcctacacc ggttaagatg ccacgcttag gtgagtccgt agcggaggga acgctaggaa
     3481 gttggctaaa aaatgaaggc gactgggtgg aaagagatga atccctggcc gaaatcatca
     3541 ccgacaagat aaatgccgaa cttccctcgc ctgttgccgg tcgcttgaca aaaattctcg
     3601 tcaaagtgga tgagacagtg cccgtaggcg cggatatcgc actcattgaa gagaacgcag
     3661 atgtgtcggc ttctccaccg gccgagcccg cgccaggtcc tgatgcagct cctattgaaa
     3721 gtcccgatcg cgcaaatgta gcggtcatgg agaacaggga agccagcgac ggcaatggtt
     3781 caagagcggc cgcgcaggcc tcgcgtgtag gagcggaaga gcgccagcgt atctctccac
     3841 tggcacgcag gctggcacgc gagcacgatc tcgacctgaa cgcgattgct ggcaccggca
     3901 ccggcggtcg ggtgcgcaaa gaagacattc tcgcctacgt ggcacagcgc caggtcgcgc
     3961 ccgctgccgc cgttgctcaa cctgtagtac ccgcgcctgc tgcccaaccg gcctcaatcc
     4021 cacctgcgcc agtacctgta gggactcaat tcactgcgac ccccgctcct gtcatggtgg
     4081 gagaagaaga ggtcgagctg atcaccccca gccgcatgcg cctggcgatt gctgaacata
     4141 tggtgcgcag caaacgcacc tccccacctg ccaccaccgt ggtcgaggtc gatatgacca
     4201 acatcgccaa atggctggag aagaacaagg aagacttcaa gaagcgcgag ggttacagta
     4261 tcagctacgt gccgttcgtt atgaaggccg tgtgcgaagg catacgcaag ttccctttga
     4321 tgaattcaag ctggacggaa gacaacaaga tcattgtgaa gaagcgcatc aaccttggca
     4381 tggcggtggc tactgatgaa gggcttgtgg tcccgacact ctacgacaca gatcaatata
     4441 cgattgccgg cctggcgaga caaatttccg ccgtcgccca gcgcgcccgc gccaataagc
     4501 tgacgataca ggatttgcag gggagcacct tcgtagtgaa taaccctggc acatttggta
     4561 caattatatc agtgccgatt atcaatcagc caaatgccgg aattctcagc atggacgcgg
     4621 tagtcaaacg gccggtggta atcgaggatg atgcgatcgc tgtgcgctcc atgatgtacc
     4681 tcagcctctc gttcgaccat cgtatcctgg atggagccgg ggccggtggt ttcttgcagg
     4741 ccgtgcgcac caaattgcaa tcctacggcc gcgagatcga tgtttattaa aaggaaaaca
     4801 acatggcgac catgattcca gaacgccgtc ctgaatggct cagggtgcgt cccccca
//
LOCUS       DASZAK010000544         6060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_85088, whole genome shotgun sequence.
ACCESSION   DASZAK010000544 DASZAK010000000
VERSION     DASZAK010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85088"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..2271
                     /locus_tag="VGT44_09565"
     CDS             325..2271
                     /locus_tag="VGT44_09565"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2581088.1"
                     /translation="MPRCPNPRCQADYPAGTYQCGNPFCLCLLPEAVVAGRYRIETLV
                     GMGGMGAVYRASDTFEMQQVAVKVFLMTQRNLDETTAVERFRREARYAHQLQHHNIVP
                     VLNFGQDGRLLYLVMPLITGGTLKGLLKAEQPLPIALTLRYLGDLADALDTIHAHPQR
                     IVHRDIKPSNLLISQDDGRLMVTDFGIARAMQKERALTQDGFALGTEHYTAPEQGMGH
                     PEPASDIYAIGVIAYQMLTGLLPFQAIVRSHGAVLPMPSEINLLVNPAADQAVMRATD
                     PDPVRRFSSAREFVDALSVALRGAPGVNTPTMLVSSGNVNVIVRTIIPENPCSECGQE
                     NRSSSRFCRRCGHRLDDTSPLVTDVCQVGYASDRGKRYVAEENEDMLLVVQGLCANLA
                     PPPRPFGLFAVADGLRGPKGKSVGGHEASRLGIETVADVLLPQLATPLPSSTTLSPGP
                     GASIQRIGLPFGDKSMLPDDTVIDQWLHEAVRRANQVIYHCNADYDTNRASTLTVALV
                     YKRRLYVASVGDSRAYFYSPGKGLKLITTDHTLGAGMVDADLLKPEEIYTSPKGKQLY
                     RYLGQEHLARIDTFHLPVAMNDAVLLCTDGLWHMLRDERIETLMAQWQADNSDPQKLA
                     RALVDEANEAGGDGNISAIVVGIM"
     gene            complement(2276..3991)
                     /locus_tag="VGT44_09570"
     CDS             complement(2276..3991)
                     /locus_tag="VGT44_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581089.1"
                     /translation="MKNKRKLLLSLIVMMSLLVNLAIVFGIFDKLLPSFAQSPGVTVT
                     DFYIASGQDPWGTTFDSKGNVWVAVPGCDPSPMCNTNTPPGKIEVYDPSTKNWTATYQ
                     LPSGYGQPLFIQFDASGNLWFAMPMSNSIGMFDPQSDTFQQYAVPTAGSGPWGLAIDH
                     NGNIWFTEHYTDQIGEFIPSTQTMKEFVTPTANSLPYGITVDASDNIWFAENNSAVAQ
                     IGEYTAGGKMEEYKIRSNPPSGLTPHMITVDPNGNIWWTEGWVGMIGELDVTKATPGT
                     NNGVTEYAYPISCNTCGEHTSGIKVDGYDQVWFDDSMQGIFGSFPDSGTGSFSTYPAP
                     TQQHPHDGLNVDAQNRIWFTEEFANKLAEAIQNGPPPTPTPSPSPSPSPTKTIARDTF
                     KRANQQFWGKASDGLTWGGDANSSSVFSINNKKGQLSNGATAYNAVLGPVVTDSEVIF
                     RGSLSSFKSANIGAVLRWTDTDDWYDVYIDGSSLVLQKDVSGVTSTLKTVAFAAAAGK
                     SYLIDFKIVGTTLSANVWKAGTTEPSGWMVTTNDSSLSSGYCGLRIQLQGSVVATIAS
                     FQAWS"
     gene            4243..>6060
                     /locus_tag="VGT44_09575"
     CDS             4243..>6060
                     /locus_tag="VGT44_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEV2581090.1"
                     /translation="MSTPASQSKSATVKKVMISSTARDLPEHRDIVKEACLRQGMFPI
                     MMEHLPASDPGALPESMRMVDEADLYLGIYAFRYGYIPKGYDISVTEMEYKRASDRGI
                     PRLIFIMDKAHPIRFEDVEMGEGAAKLDAFKTRLKTDHVVNFFHSPQELQAQVINTLS
                     QHRQPDLTAFHYVSDIPEPPEPYIAHPYTLLQTPNLIGRQGALNLLTDWVAKPGSVLY
                     QARILSVVAIGGMGKSALTWKWFQDISPNEMHPLAGRLWWSFYESDASFDNFVMRALA
                     YASHRIQEEIQKLTPPEREAQLLSILDREPFLLVLDGLERLLIAYARMDAARLLDDDL
                     DERTANFVTGALGLPKSAAQSFIGQHRLRKTADLRTGAFLRKLATVRASRILVSTRLY
                     PADLQTDTGTERAGSQAIFLQGLPDDDALNLWRTLKITGSRETLVPLFRTFENHPLLI
                     QVLAGEIAHDRRAPGDFDTWRKTHPNFDPFRLSLVQVKSHVLEFALRGLDETAQKVLT
                     TIAAFRMPAAYDTLVDLFVGEGKPCSDETDLIGILADLEDRGLLGWDRRANRYDLHPI
                     VRGVTWEGLGKEAQLGIYETLGAHFESLPTIDDEEVERLE"
BASE COUNT         1258 a         1850 c         1687 g         1265 t
ORIGIN      
        1 tccctccctc ccagccgacc agctcacacc tctgcctcct gatctgctcc tgagccgcaa
       61 ggcgaattat cgcctctaca agccgcgtcc gccccgtccc gcctccaatc gcaagaagaa
      121 gaaagcctcc gctcccacgt ctccctcttc ccgaaaggct cgataaatga ttttcttaag
      181 accaatgtcc cttgcggttg ccatgcttct ctcgactgtg cttcagcgct gattgatgaa
      241 tgatgttata ttgacatccc tcgctcatca ccctacaata aaaacgtaat taaacgcttt
      301 gacgaatgtc cttaaggaaa gagtatgcct cgttgtccga atccacgctg ccaggccgat
      361 tacccggctg ggacgtacca gtgcggcaat ccgttttgcc tgtgtttgct gccggaagcc
      421 gttgtcgcgg gccgttaccg cattgagacg ctggtcggca tgggtggaat gggggcggtg
      481 taccgcgcca gcgatacgtt tgagatgcag caggtcgccg tcaaggtctt tttgatgacg
      541 cagcgaaacc tggatgagac gacggcagtt gagcggttcc ggcgcgaggc gcgctacgcg
      601 caccagctcc agcaccacaa tatcgtgccg gtcctcaatt tcgggcagga tgggcgcttg
      661 ttatacctcg tcatgcctct gattaccggc ggaacgctga aagggctgtt gaaggccgag
      721 cagccgctgc ctatcgcact gacgctgcgc taccttggcg acctggccga cgcgctcgac
      781 accattcacg cgcacccgca gcgcatcgtg catcgcgata tcaagccctc caacctgctc
      841 atctcgcagg acgatgggcg gctgatggtg acggattttg gcatcgcgcg cgccatgcag
      901 aaggaacgcg ccctgacgca ggatggcttc gcgctcggca ccgagcatta taccgcgccc
      961 gaacagggca tggggcatcc cgaaccggcc agcgatatct acgccatagg ggtgatcgcc
     1021 tatcagatgc tgaccggtct gctgcccttt caggccatcg tgcgcagtca cggggcggtg
     1081 ctgcccatgc ccagcgagat caatctgctc gtcaacccgg cggcagacca ggcggtcatg
     1141 cgcgctaccg accccgaccc ggtcagacgt ttcagctccg cgcgcgaatt tgtcgatgcg
     1201 ctcagcgtcg cgctcagggg ggcaccgggt gtcaacaccc cgaccatgct ggtatcgtcg
     1261 gggaatgtga atgtgatcgt gcggaccatt atccccgaaa atccctgtag cgagtgcggg
     1321 caggagaacc gcagcagctc gcgcttctgc cgccgctgcg ggcatcgcct ggacgacacc
     1381 tcgccgctcg tgacggatgt ctgccaggtg ggctacgcca gcgatagagg caagcgctat
     1441 gtcgccgaag agaacgagga tatgctgctg gtggtgcagg gcctttgcgc caatctcgcg
     1501 ccgccgccgc gtcctttcgg cctcttcgcc gtcgccgatg gcctgcgcgg gccaaagggg
     1561 aaatccgtcg gcggtcacga agcgagccgc ctgggcatcg agaccgtcgc ggacgtgctg
     1621 ctgccccaac tcgcgacccc gctgccgtct tctactacgc tctcgcccgg cccaggcgcg
     1681 tccattcagc gcatcggtct gccattcggc gataaatcca tgctgcccga tgataccgtc
     1741 atcgaccagt ggctgcacga ggctgtgcga cgcgccaacc aggtgattta ccactgcaac
     1801 gccgactacg atacgaaccg cgccagcacg ctcaccgtcg cgctcgtcta caagcgccgt
     1861 ctctacgttg ccagcgtcgg cgatagtcgc gcgtatttct acagtcctgg caaagggttg
     1921 aaactcatca ccaccgacca cacgcttgga gcagggatgg tggatgccga tctgctcaag
     1981 ccggaggaga tctataccag cccgaagggt aaacaacttt atcgttacct gggacaagaa
     2041 cacctggcgc gcatcgatac cttccacctg cccgtcgcga tgaacgacgc ggttttactc
     2101 tgcaccgacg gcctgtggca catgctgcgt gacgagcgca tcgaaacgct gatggcgcag
     2161 tggcaggccg acaacagtga tccgcaaaag ctggcgcgcg cgctcgtcga cgaggccaac
     2221 gaagcgggcg gcgacggcaa tatcagcgcg attgtggtcg gcattatgta acaggttatg
     2281 accacgcctg gaacgaagca atggttgcca cgacgctgcc ctgcaactgg atacgcaagc
     2341 cgcaatagcc cgatgagagc gagctatcgt tcgttgttac catccagccg gacggctcgg
     2401 ttgtgccggc tttccacacg ttggccgaca gagttgtccc aacgatcttg aagtcaatca
     2461 agtaggattt accggcggcg gcggcaaacg caacggtttt cagcgttgaa gtgacgccgc
     2521 tcacatcttt ctgcaagacc aggctgctgc catcgatgta gacatcgtac cagtcgtcgg
     2581 tatccgtcca gcgcaatacc gctcctatgt tggcgctttt gaagctgctc aacgatcctc
     2641 tgaagatcac ttcggagtcg gtcacgacgg gtccaagcac agcgttatag gcggttgctc
     2701 cgtttgacaa ctgccccttt ttgttgttga tcgagaagac gctggacgag ttggcgtcgc
     2761 cgccccaggt aagtccatcg ctggctttcc cccagaactg ctggttggcg cgcttgaacg
     2821 tatcacgagc aatcgtcttt gtgggtgacg gactgggtga agggctgggt gtcggcgttg
     2881 gcggcgggcc attctggatg gcttcggcca gtttattggc gaactcttcc gtaaaccaga
     2941 tgcggttttg cgcatcgacg ttcagcccat catgcgggtg ctgctgcgtg ggcgcgggat
     3001 aggtggaaaa tgagcctgtc ccagaatcag ggaacgagcc gaagatgccc tgcatcgaat
     3061 cgtcgaacca gacctggtca tatccatcta ctttgatgcc ggatgtatgt tccccacagg
     3121 tgttgcagga tatgggatag gcgtactcgg tgacgccgtt attcgtgccg ggcgttgctt
     3181 tcgttacatc aagctcgccg atcatgccca cccatccttc ggtccaccag atgttgccat
     3241 tcgggtcaac agtaatcatg tgcggcgtca ggccgctggg cgggttgctg cggattttgt
     3301 actcctccat cttcccgcca gcagtgtatt cgccgatttg cgccaccgcc gagttgtttt
     3361 cggcaaacca gatattatcc gaggcatcaa ctgtgatgcc atacggcagg ctattggcgg
     3421 tcggcgtcac aaattctttc atggtctggg tcgagggaat aaattcgccg atctggtcgg
     3481 tatagtgttc ggtaaaccag atgttgccat tatgatcgat ggcaagaccc cagggtccag
     3541 acccggcggt tggcacagca tactgctgga atgtatctga ctgcggatcg aacatcccga
     3601 tggaattcga catgggcatg gcgaaccaga ggttgccgct ggcatcaaac tggatgaaga
     3661 gcggttgccc gtaacccgaa ggaagctggt aagtcgcggt ccaatttttc gtagagggat
     3721 catagacctc aattttgccg ggtggagtat tagtgttgca catcggcgat ggatcgcacc
     3781 ccggaacagc tacccacaca ttgcccttgc tatcgaacgt cgtcccccag ggatcctgcc
     3841 cgctggcgat atagaaatcg gtgacagtga cgccgggcga ttgggcaaag ctgggcaaca
     3901 acttgtcgaa aatgccgaaa acaatggcga gattgaccag cagcgacatc ataactataa
     3961 gactaagcag taattttcgt ttatttttca tacgcgctta tgttccagtc cagtgaaatg
     4021 aaaaaccttc actgaaacag tatgatacag ccgcttgatt gaaacaagtt attcggtatc
     4081 aactctgtga gcctatcggt tcagcatagt gagggttggc gttaatcgaa gaggacgagg
     4141 gcgaccatat atccataatg cttgtgtaat cgcatgttgc acagtataat catgcagagc
     4201 ttcaattcgc gtttcgtcgc atatccaggt gggagggcat ctatgtcaac gcccgcatcg
     4261 cagtcaaaat cagcaaccgt caaaaaagtc atgattagca gcaccgcccg cgatctgccc
     4321 gaacatcgcg acatagtgaa ggaggcatgc ctgcgccagg gcatgttccc gatcatgatg
     4381 gaacacctgc cagccagcga ccccggcgca ctgccagaat cgatgcgcat ggtcgatgaa
     4441 gccgacctct acctcggcat ctacgccttc cgctacggct acatccccaa aggctacgac
     4501 atctccgtca ccgaaatgga gtacaaacgc gccagcgatc gcggcatccc gcgcttgatc
     4561 ttcatcatgg ataaggcgca tcctattcgc tttgaagatg tggagatggg cgagggcgca
     4621 gcgaagctcg atgccttcaa aacccgcttg aagaccgatc atgtcgtcaa cttcttccat
     4681 tcgccccagg aactgcaagc ccaggtcatc aacaccctct cacagcatcg ccagcctgac
     4741 ctgaccgcct ttcactacgt cagcgacatc cctgagccgc ctgaacccta tatcgcccat
     4801 ccctacacac tgctacaaac tcccaacctg atcgggcgac aaggcgcatt gaacctgctc
     4861 accgactggg tcgccaaacc cggctcggta ctctaccagg cgcgcattct ctcggtcgtg
     4921 gccatcggcg gcatgggcaa aagcgctctc acctggaagt ggttccagga catctccccc
     4981 aacgaaatgc atccgctcgc aggccgcctg tggtggagct tctacgagag cgatgccagc
     5041 ttcgacaatt tcgtcatgcg cgccctggcc tacgctagcc accgcatcca ggaggaaatc
     5101 caaaaactca caccaccgga acgtgaagcg caactgctct ccatcctcga ccgtgaaccg
     5161 ttcctgctcg tgctggatgg cctggaacgc ctgttgatcg cctacgcgcg tatggacgcc
     5221 gcgcgcctcc tcgacgacga cctcgacgag cgcacagcca acttcgtcac gggggcgctt
     5281 ggcttaccaa agagcgcggc gcaatccttt attggccagc accgcctgcg caagaccgcc
     5341 gatctgcgca cgggtgcctt tctgcgcaag ctggccaccg tccgcgcttc gcgcatcctc
     5401 gtcagcaccc gcctctatcc cgcggacttg caaaccgaca caggtacgga acgcgccggg
     5461 tctcaggcga tcttcctcca gggtctccct gacgatgacg cactcaattt gtggcgaact
     5521 ctcaagatca ccggctcgcg tgagacactt gtgccgctct tccgcacatt tgagaaccat
     5581 cccctgctca tccaggtgct ggcgggcgag attgcgcacg accgccgcgc acctggtgac
     5641 tttgatacct ggcgcaagac ccaccccaac ttcgatccgt ttcgcctgtc gcttgtgcag
     5701 gtgaagtcac atgtgcttga attcgcttta cgaggtttag atgaaaccgc tcaaaaagtc
     5761 ctcacgacca tcgcggcctt tcgaatgccc gctgcctacg atacgcttgt cgatctattc
     5821 gtcggggaag gcaagccttg ctccgatgaa accgatttga tcgggattct ggctgacctg
     5881 gaagatcgcg gtttgctcgg ttgggaccgg cgcgccaacc gctacgacct gcaccccatt
     5941 gtgcgcggtg taacctggga gggcttgggt aaggaggctc agcttggtat atatgagacg
     6001 cttggcgcac atttcgagtc cttgcccacg atcgacgatg aggaggtcga gcgcctggaa
//
LOCUS       DASZAK010000545         2155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_189794, whole genome shotgun sequence.
ACCESSION   DASZAK010000545 DASZAK010000000
VERSION     DASZAK010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189794"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..444
                     /locus_tag="VGT44_09580"
     CDS             34..444
                     /locus_tag="VGT44_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581091.1"
                     /translation="MKANDYQTYQVAISPDLDISAEEFAIAWNADPDARDLATAHFAE
                     EKAAQFIDPTLLVAALLSVPANVASAALYDLMKAVIERLRNEKEQAPALQASPAQPAS
                     PAPQSPQTPRKHLHIEQTRKADGSTIIIVDYEEA"
     gene            471..>2155
                     /locus_tag="VGT44_09585"
     CDS             471..>2155
                     /locus_tag="VGT44_09585"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEV2581092.1"
                     /translation="MPQLISIQQRGEQPDEAGNWQAVVRINNGPWNPITISNPFSGAE
                     EIELAWYFEEHLAFPFTGKVRASNAAISIKTYGEQLFNQVFAQNARVEFAYHKAVEAG
                     LSDVQIEIEGWPAFHALHWEALRDSELPEPLVLQATMVRKNIVPSTVQSQVRPSPTIN
                     LLIVTARTHGKRDVGYRTISRPLVEGLRKTGVPIQIDILRPGTYRTLVNHLNDVTAKH
                     GEGYYHVVHFDVHGSILTYEQLQRGHQANRFVFNQRFARADIQPYQDVKAFLSFESEQ
                     EDNQSDLVEASELAALLLKHHVPITILNACQSGMQIGASETSLGSQLMQAGVQLVLAM
                     GYSVTVSAAELLMRTLYQKLFDHDDLKVAIRHARTELYNNKARQAYSDQQIDLEDWLL
                     PVVYQNQPVQLKPREFMPEERSAYNKRKLAEKQYTPPEPQYGFVGRDLDILQIEKRLL
                     AKRNLLLIRGMGGAGKTTLLKHLRAWWHTTGFAQQTFYFGYDEQAYTLQRILTAIAQQ
                     LYGAKYYTDIQPYLLDEQHVMIAERLRAEPHLLILDNLESITGAHLAIQHTLP"
BASE COUNT          518 a          717 c          550 g          370 t
ORIGIN      
        1 atagatatat cacaagggag ttacagcgac tatatgaaag caaacgatta ccagacctac
       61 caggtcgcca tctcgcccga tctcgacatc agcgccgaag aattcgctat cgcctggaac
      121 gctgaccccg atgcccgcga ccttgctacc gcacatttcg ccgaggaaaa agccgcgcaa
      181 ttcattgacc caacgctgct ggtggctgcc ctgctcagcg ttcctgccaa cgtcgccagc
      241 gccgcgctct acgaccttat gaaagccgtc atcgagcggc tgcgcaacga aaaagaacag
      301 gcaccggcgc tacaggcatc accggcgcag ccagcctcgc cagcacctca atctccgcaa
      361 acgccgcgca aacatttgca tatcgagcaa acgcgcaagg ccgatggatc gaccatcatc
      421 atcgttgatt acgaggaagc ataaaatacc cgtcgatagg agacgtggac atgccacaac
      481 tcatcagcat tcaacagcgt ggcgagcagc ccgacgaagc aggcaactgg caggcggtcg
      541 tgcgcatcaa caacgggccg tggaatccca tcactatcag caatccgttt tcgggcgcag
      601 aagaaataga gcttgcatgg tacttcgagg aacacctggc attcccgttc accggcaaag
      661 tgcgcgccag caatgccgct atcagcatca agacctatgg ggagcagctg ttcaaccagg
      721 tcttcgcaca aaatgcgagg gtggagttcg cttaccacaa ggcagtggag gccggattga
      781 gcgatgtgca gatcgaaatc gaaggctggc cagcctttca cgccctgcac tgggaggcgc
      841 tgcgggactc ggaactgccc gaaccgctgg tcttgcaggc caccatggta cgcaagaata
      901 tcgtcccctc aacagtccag tcgcaagtac gtccatctcc taccatcaac ttgctgatcg
      961 tcacggctcg cacgcatggc aagcgcgacg tgggctatcg taccatctcg cgccccttag
     1021 tagaaggact gcgcaagaca ggtgtgccaa tccagatcga tatcctgcgt cccggcacct
     1081 atcgtacgct ggtgaatcac ctgaacgatg tcacggccaa acacggcgag ggctactatc
     1141 acgtcgtcca cttcgatgtg cacggttcca tcctcaccta cgagcagctc caacggggac
     1201 accaggccaa ccgcttcgtc ttcaaccagc ggttcgctcg cgcggatatc cagccatacc
     1261 aggacgtgaa agccttcctt tccttcgaga gcgaacagga agacaaccaa tccgacctgg
     1321 tcgaagcctc ggaacttgca gctctgctgc tcaaacatca cgtccccatc accatcctga
     1381 acgcctgcca atccggtatg cagatcggcg cgagcgaaac cagcctgggc agccagttga
     1441 tgcaggccgg tgtccagctt gtgctggcga tgggctattc cgtcaccgtc agcgccgctg
     1501 aactgctgat gcgcactctc taccagaaac tcttcgacca cgacgatctg aaagtcgcca
     1561 tccgccatgc gcgcaccgaa ctctacaaca ataaagcgcg ccaggcctac tccgaccagc
     1621 agattgatct ggaagactgg ctgctgcccg ttgtctacca gaaccagccc gtgcaactca
     1681 aaccgcgcga gttcatgccg gaggaacgca gcgcctacaa caaacgtaag ctggccgaaa
     1741 agcagtacac gccgcccgaa ccgcagtatg gcttcgtagg ccgcgacctc gacatcttgc
     1801 agatagagaa gcgcctgctg gcgaagcgca atctcctgct cattcgcggc atgggcggcg
     1861 cgggcaagac cacgctgctc aagcacctgc gcgcctggtg gcacacgaca ggcttcgcgc
     1921 agcagacctt ttacttcggc tacgacgagc aagcctacac gctccagcgg atcctcaccg
     1981 caatcgccca gcagttgtac ggcgcgaaat actacaccga catccagccc tacctgctcg
     2041 acgagcagca tgtcatgatt gccgaacgcc tgcgcgccga gccgcacctg ctcatcctcg
     2101 acaacctcga atcgatcacc ggcgcgcacc tcgccatcca gcacaccctg cccaa
//
LOCUS       DASZAK010000546         1756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_296817, whole genome shotgun sequence.
ACCESSION   DASZAK010000546 DASZAK010000000
VERSION     DASZAK010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296817"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /gene="rpsM"
                     /locus_tag="VGT44_09590"
     CDS             <1..131
                     /gene="rpsM"
                     /locus_tag="VGT44_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV2581093.1"
                     /translation="CYRGMRHRRNLPVRGQRTRTNARTRRGPKRTVAGKKKATAKK"
     gene            155..559
                     /gene="rpsK"
                     /locus_tag="VGT44_09595"
     CDS             155..559
                     /gene="rpsK"
                     /locus_tag="VGT44_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020449874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV2581094.1"
                     /translation="MAAEKKKTTGGRPRRRERKSVPRGQAHIQATFNNTIVTLTDPNG
                     NVISWGSAGGHGFKGSRKSTPYAAQVTAESAARKAMDNGMKQIEVYVKGPGSGREAAI
                     RSLQATGLNITSITDITPIPHNGCRPPKRRRV"
     gene            578..1204
                     /gene="rpsD"
                     /locus_tag="VGT44_09600"
     CDS             578..1204
                     /gene="rpsD"
                     /locus_tag="VGT44_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV2581095.1"
                     /translation="MARYTGPVCKLCRREGVKLFLKGDKCLMKCTLERRNTRPGQHGT
                     GRPRKPSGYALQLREKQKVRRTYGLLERQFRKNFETAARRTGRTSENLMQILEMRLDN
                     LVYRLGFADSRAQARQLVNHGHFNVNNRKVDIPSYIVKPNDVIAVRDRSKGLEYFKTR
                     ALMLSSKGISPWLSLNIEGMSGRVMSMPSRTELELPFDEQMVVEYYQR"
     gene            1384..>1756
                     /locus_tag="VGT44_09605"
     CDS             1384..>1756
                     /locus_tag="VGT44_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV2581096.1"
                     /translation="MTLGNALRRVLLSSLPGAAVTSIRIDGVQHEFQDVPNVTEDVTE
                     IVLNVKKLRLRSFSDHPVSMRLEVSGERVVTAADILAPSTVEIVNPDLYIATLDNDDA
                     RLEMELVVETGKGYVPADSKED"
BASE COUNT          425 a          456 c          518 g          357 t
ORIGIN      
        1 gctgttatcg tggaatgcgc catcgccgca atcttcccgt gcgggggcag cgtacacgca
       61 caaacgcgcg gacgcgccga gggccgaaac gaacggtggc aggcaagaag aaggctactg
      121 ctaagaagta actccgagaa ggtaaagaaa gcaaatggca gcagagaaaa agaaaacaac
      181 cggcggcagg cccaggcggc gtgagcggaa atcggtgccg cgtggccagg cgcatatcca
      241 ggcaaccttt aataatacaa ttgtgaccct gaccgatccc aatggaaacg tgatttcatg
      301 gggcagcgcg ggtgggcatg gcttcaaagg ctctcgcaag agtaccccct acgcggcgca
      361 ggtcacagcc gaatcggcgg cgcgcaaagc gatggataat ggcatgaaac agatcgaggt
      421 ctatgtcaaa ggaccgggtt ctggccgtga agccgccatc cggtcgctac aggctacggg
      481 gctgaatatt acatcgatta ccgatattac acccattccg cacaatggct gccgcccgcc
      541 caagcggcgt cgcgtgtagg tcaggaggat ataatacatg gcgcgttata ccggccccgt
      601 ctgcaagctg tgcaggcgcg agggggtaaa actgtttctc aagggcgaca agtgcttgat
      661 gaaatgcact ctggaacgcc gtaatactcg tcccggccag cacggcacgg gccgcccgcg
      721 caagccgtcg ggttatgcgc tgcaattgcg cgaaaagcag aaggtacgcc gcacctacgg
      781 attgctggaa cgccagttcc gcaagaattt tgagacggct gccaggcgca caggccgcac
      841 cagcgaaaac ctcatgcaaa tccttgaaat gcgcctggat aacctggtgt atcgtctcgg
      901 ttttgccgat tcgcgtgcgc aggctcgcca gctcgtcaat cacggtcact tcaatgtaaa
      961 taatcgtaaa gtcgatattc cttcgtatat tgtcaaaccg aacgatgtga ttgctgttcg
     1021 cgaccgcagc aagggtctgg aatactttaa aacccgcgcc ctgatgctat cctcaaaagg
     1081 catttcgccc tggttgagcc ttaatatcga gggcatgagt ggtcgggtga tgtctatgcc
     1141 gtcgcgcaca gaacttgagt tgccctttga tgaacaaatg gtggttgagt attaccagcg
     1201 gtaagcgccg cttctcgctg gtcgatgcat atcgatgaac aggtgtgcag gctttgaagc
     1261 agcgatagga aagaaaggca ggtaggaacc ttgctagaaa ttactctgcc tcgcattaaa
     1321 aatacaaaaa cacagggaaa ctatgcgagt tatgatatcg agcctttaga ggcggggtac
     1381 ggcatgacgc tgggcaatgc gctgcggcgc gtcttgctct cctcgctgcc tggtgcggcg
     1441 gtgacctcta ttcgcatcga tggtgtgcag catgagtttc aagatgtccc caatgtcacg
     1501 gaggacgtga ccgagatcgt cctgaacgtc aagaagctgc gcctgcgctc cttttcggac
     1561 catcccgtat cgatgcgtct ggaggtgagc ggtgagcgtg tggtgacggc agcggatatc
     1621 ctggctccga gtacggtgga gatcgtcaat cctgatctct atatcgctac tctggataat
     1681 gacgatgctc gcctcgagat ggaactggtc gttgagaccg gtaaaggcta tgtccctgcc
     1741 gactcgaaag aagatc
//
LOCUS       DASZAK010000547         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_440645, whole genome shotgun sequence.
ACCESSION   DASZAK010000547 DASZAK010000000
VERSION     DASZAK010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440645"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /gene="rplC"
                     /locus_tag="VGT44_09610"
     CDS             <1..712
                     /gene="rplC"
                     /locus_tag="VGT44_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV2581097.1"
                     /translation="TQIKKKTASDKYDAIQIGFEQKLARRATRPELGHLGHSLPLLKG
                     QGKRLRAYQQSQRGKKAAEGETESEEAGAKKDKQIEKLLRVSQNRQRRPGPEVGPFKV
                     LREVKLQEGNSPDDIELGEKFDVSLFRVGEEVDIVGTSKGKGFQGGVKRWGFAGGPKT
                     HGQSDRTRAPGSIGSGTTPGRVLKGTHMAGRMGNAQITMKSLQVIQADPERNLLVVKG
                     SVPGANGGLLMIKKHVMR"
     gene            719..1510
                     /gene="rplD"
                     /locus_tag="VGT44_09615"
     CDS             719..1510
                     /gene="rplD"
                     /locus_tag="VGT44_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV2581098.1"
                     /translation="MPSTTVYNMAGEVIEQLELSEQVFGVTPNVAVLHQVVTAQLVNR
                     RQGNASTKTRGEVSGGNKKPYKQKGTGHARQGSTRAPQFRHGGVVFGPRPHKYHHDVP
                     KKMRRLAIRSALSDKVANEQLIVLDELSMESPRTKDMLALLGRLPLEGKKVLMMLPSR
                     DENIVLSTRNIPRAKVQHVSSINVIELLKHDFVIMPRQTVRWIELVFGEGVSAEEASQ
                     QIASQLEAEDVSEGSEEPAVESNEPASEETADLESGSSETNGEEE"
     gene            1514..1816
                     /gene="rplW"
                     /locus_tag="VGT44_09620"
     CDS             1514..1816
                     /gene="rplW"
                     /locus_tag="VGT44_09620"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV2581099.1"
                     /translation="MEVTEILRHGIVTEKATRLQNENNQYTFKVALQANKIEIRKAVE
                     QMFKVHVVKVNTMRMPGKTRMLRRRGGRAPQVMEAREWKKAIVTLAPGDSIDILKA"
     gene            1898..2734
                     /gene="rplB"
                     /locus_tag="VGT44_09625"
     CDS             1898..2734
                     /gene="rplB"
                     /locus_tag="VGT44_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV2581100.1"
                     /translation="MPIKQYRPTSPGRRGMSVSTFEEITKTKPEKSLTVRLKKHAGRN
                     NQGKITVRHRGGGAKRAYRIIDFKRNKLGVPAKVAAIEYDPNRSARIALLHYVDGEKR
                     YIIAPVGLKVGDRVVSGPEADIRPGNALPLKNIPAGTTVHNIELQRGRGGQMVRGAGA
                     GAQLMAKDGDYALLRLPSGEQRKVHILCMATIGQVGNTDHENINLGKAGRSRHMGRRP
                     TVRGSVMNPRDHPHGGGEGRAPIGGQPQTPWGKPALGYKTRHNKRTDKYIVRRRNAGR
                     RK"
     gene            2738..3025
                     /gene="rpsS"
                     /locus_tag="VGT44_09630"
     CDS             2738..3025
                     /gene="rpsS"
                     /locus_tag="VGT44_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV2581101.1"
                     /translation="MSRSRKKGPYIHESLRKKVLTMLKSGDRKVIKTWSRASTIFPQM
                     VGMTIGVHNGKTHIPIYITENMVGHKLGEFAPTRHFRGHAGGKSEKTTSVR"
     gene            3034..>3349
                     /gene="rplV"
                     /locus_tag="VGT44_09635"
     CDS             3034..>3349
                     /gene="rplV"
                     /locus_tag="VGT44_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV2581102.1"
                     /translation="MQVRAIAKDIGISPRKMRLVTNAVKGLRVNEALAYLQFIPNAGA
                     KPVAKVVASAAANAENNYSLDPDSLYILNIVADDSFRIKRVKARPHGRAGRILRRFCH
                     ITV"
BASE COUNT          832 a          883 c         1034 g          600 t
ORIGIN      
        1 gacgcagatc aagaagaaaa ctgccagtga taaatacgat gccatccaga ttggcttcga
       61 gcagaagctc gcccggcgag caactcggcc agagctgggg catcttggac atagtctgcc
      121 cttgctcaag gggcagggca agcgtttgcg agcataccag cagtcgcagc gcggtaagaa
      181 ggctgccgag ggcgagacag aatctgaaga agcgggagcg aagaaggaca agcagatcga
      241 aaagctgctg agggtttcgc agaaccgcca gcgtcgtccg gggccggaag tcggtccgtt
      301 caaagtgctg cgcgaagtga agttgcaaga gggaaacagc ccggacgata tcgagctggg
      361 cgaaaaattc gatgtaagtc ttttccgcgt cggcgaagag gtcgatatcg ttggcacgtc
      421 gaaaggtaaa ggcttccagg gtggcgtcaa gcgctgggga ttcgccggcg gcccgaaaac
      481 gcatggccag tcggaccgca cgcgcgcccc tggctcgatt ggttctggca ccacgcctgg
      541 ccgcgtactg aaaggtacgc atatggccgg tcgtatggga aatgcccaga tcaccatgaa
      601 gagtctgcaa gtaattcaag ctgatcctga gcgcaatctg ctcgttgtca aaggctcggt
      661 gcctggcgcg aacggtggtc tgctcatgat taagaagcat gtgatgaggt aaaccgcaat
      721 gccctcgact acagtgtata acatggccgg cgaagtcata gaacagctcg aactgagcga
      781 gcaggtgttc ggcgtgaccc ccaacgtcgc ggtactccac caggttgtca cagcgcaact
      841 ggtcaaccgc cgccagggga atgcctcaac caagactcgc ggtgaggttt cgggcggcaa
      901 caagaagccg tataagcaga aaggcacggg ccacgctcgc cagggcagta cgcgcgcccc
      961 gcagttccgt cacggcggcg tggtgttcgg cccaaggccg cataaatatc atcatgatgt
     1021 gccaaagaag atgcgccgcc tggccatcag gtccgcgctt tccgataaag tcgcgaacga
     1081 gcagttgatc gtacttgatg aactgtcgat ggaatcgccc aggacaaaag acatgctggc
     1141 gctgctgggc cgtctgccac ttgaaggcaa aaaggtgctg atgatgctgc ccagccgcga
     1201 tgagaatatt gtgctatcga cgcgcaatat tcccagggcg aaggtgcagc atgtctcgtc
     1261 gatcaacgtc atagaattgc tcaagcatga tttcgtgatt atgcccaggc aaacagtgcg
     1321 ctggatcgaa ctggtcttcg gcgaaggcgt gagcgcagag gaagcaagcc agcaaatcgc
     1381 ttcacagctg gaggccgagg atgtctccga gggatcggaa gaaccggcgg tggagtcaaa
     1441 cgagccagcc agtgaagaaa cggcagacct ggagagcggg tcgagcgaga cgaacggcga
     1501 ggaggaataa gcaatggaag ttaccgaaat cctgcgtcac ggaatcgtca ccgaaaaagc
     1561 gacccggctg cagaacgaga ataatcagta taccttcaaa gtcgcgctgc aagcgaataa
     1621 aatcgaaatt cgcaaagcgg ttgagcagat gtttaaggtg cacgtggtca aggtcaatac
     1681 aatgcgtatg cctggcaaga cccgtatgct gcgacgaaga ggcggtcgcg ccccccaggt
     1741 tatggaggcg cgcgagtgga aaaaggcgat tgtcacgctt gcgccgggtg actcaatcga
     1801 cattttgaag gcgtaaggag cctccttgca cacctcctga catgaagtgt agctgcgcag
     1861 cagcgttgga ggtgtggata gaaaaggatt tatcgagatg ccaatcaaac agtatcgccc
     1921 aacgtctcct ggccgtcgcg gtatgtcggt ttcgacattt gaagagatca ccaagacgaa
     1981 gccggagaaa tccctgaccg tcaggctgaa aaagcacgcc gggcgcaata accagggcaa
     2041 gatcaccgtg cgccatcgcg gtggtggcgc caagcgcgcc tatcgcataa tcgatttcaa
     2101 gcgcaacaag ctcggcgttc cggcgaaggt ggccgcgatc gaatacgacc cgaatcgctc
     2161 ggcgcgtatc gcgctgctgc actatgtaga tggtgagaag cgctatatca tcgctccggt
     2221 cgggctgaag gtcggagaca gggtcgtctc cggtccagag gccgatatca ggccgggtaa
     2281 cgcgctgcct ttgaagaata ttccggcagg caccaccgtc cataacattg aattgcaacg
     2341 cgggcgcggt ggccagatgg tgcgcggcgc gggcgcgggc gcgcagttga tggcgaaaga
     2401 cggcgattat gccttacttc gcctaccttc gggcgagcag cgcaaggtgc atatcctatg
     2461 catggcaacc atcggacagg ttggcaatac cgaccatgag aacatcaatc tgggtaaggc
     2521 cgggcgttct cgccatatgg gtcgtcgtcc gacggtgcgc ggctccgtga tgaacccccg
     2581 cgaccatccg cacggcggtg gcgaaggtcg cgctcctatc ggtgggcagc cgcagacgcc
     2641 ctggggcaaa ccggccctgg gctataaaac gcgccacaat aagcgcaccg acaagtacat
     2701 tgtgcgccgt cgcaatgctg gcaggaggaa gtagcacatg tcgcgctcaa ggaagaaggg
     2761 gccgtacatt cacgagtccc ttcgcaaaaa ggtcttgacg atgttgaaaa gcggcgaccg
     2821 caaggtcatc aagacctggt cgcgcgcctc gaccatcttc ccacaaatgg tgggaatgac
     2881 aattggcgtc cacaatggca agacgcatat tcccatctat ataacagaaa atatggtggg
     2941 gcataagcta ggcgaatttg cccccacgcg gcacttccga ggtcacgcgg gcggtaaaag
     3001 tgaaaagacc acatcggtcc gctgaggtaa aagatgcaag tacgagctat tgcgaaagac
     3061 attggtatat ctccgcgtaa gatgcgcctg gtaacgaatg cggtaaaggg actgcgcgtc
     3121 aatgaggcgc tggcctatct ccagttcata cccaacgcgg gcgcgaagcc ggtcgcgaaa
     3181 gtcgtggcct cggcggcagc caatgcggaa aacaactata gtttagatcc tgattcattg
     3241 tatatcctga atattgtggc ggatgactcg tttcgcatca agcgcgtcaa ggcgcgaccg
     3301 catggtcggg ccggtcgcat tttgcgccgg ttctgccata tcacagtca
//
LOCUS       DASZAK010000548         2401 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_296833, whole genome shotgun sequence.
ACCESSION   DASZAK010000548 DASZAK010000000
VERSION     DASZAK010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296833"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(233..592)
                     /locus_tag="VGT44_09640"
     CDS             complement(233..592)
                     /locus_tag="VGT44_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV2581103.1"
                     /translation="MFTVLGEVPILTINMGNLHLALYLSQVMYLLVAAIIGFVAEFIV
                     GWRLPFGIIGAIVAALVGIWIVTNIITLIIPGDPVLFGVPLFKTLIGATLFAALWYLL
                     TYRLWHHRRPYYRRAVA"
     gene            complement(765..2144)
                     /locus_tag="VGT44_09645"
     CDS             complement(765..2144)
                     /locus_tag="VGT44_09645"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2581104.1"
                     /translation="MVALPGENSPDNQDGDIFALEPLKPFELERGERLLYRLTEFGRI
                     LWEVGIDVGPRKMLDLAETLAFVDLTYREDFYNALKCSLLAKHEQEPLFDQMYMYYWH
                     MRDQLGKKKPEAPGTARRDEKRLRLPPSELKRLAEHMNAPQEQRKDLRAEMRESERRR
                     RDDERRSEESDDDTATPQGLAYSAIEALRKKDFESFSWDEVQEAKKLMAEMRWHLGLR
                     PTRRKRPSRRGSYPDMRRIVRRNLKYGAELLELTWRETKRKPRPLVIICDISGSMSLY
                     SRLLLHFIHTISNGLLNVEAFVFGTRLTRVTRQLKKRDVDDAVRDVSKSVQDWSGGTR
                     IGDALHFFNHKWARRVLGRGAVVLIISDGWDRGEPGMLEVEMDRLQHSCHRLIWLNPL
                     LGSPDYRPLTIGMKTALPYIDNFLPAHNLDSLIQLGNLLATIDDSRPIRDAATRRKAV
                     EEWSKRPPM"
     gene            complement(2144..>2401)
                     /locus_tag="VGT44_09650"
     CDS             complement(2144..>2401)
                     /locus_tag="VGT44_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEV2581105.1"
                     /translation="PGVPERLAKQVCAFVRELRSADLYKAPGVAETLDWISSLLALDQ
                     IDLVDGPVRDTLGALLKYQDDVLKIGGSEFTRYLKKAQGVS"
BASE COUNT          496 a          701 c          704 g          500 t
ORIGIN      
        1 attgtagcgg gcgtagtcgt cagtgtgcga ctctacaatc aaggtctttt cgaaacgggc
       61 cgtttcaggc gtattcgacg ggtacgctca tcccccgacg gtcccgtcat tgaagagatc
      121 atcgaggaag cagtacccgt cgaagaagag gtttaaacac ccgatctcac cagcaatgga
      181 ggtttcccct gttggaacac aacaggggaa acctccattt ccgtcccctc gcttatgcga
      241 cagcgcggcg atagtagggg cggcggtggt gccagagtcg gtaggtcagc aggtaccaga
      301 gcgccgcgaa caaggtcgct ccaatgagcg tcttgaagag tggcaccccg aagagtacgg
      361 gatcaccagg aataatcagg gtgattatat tggtgacgat ccagatgccc accagagcag
      421 ccacgattgc cccgatgatg ccaaagggaa gccgccagcc gacgatgaat tctgccacaa
      481 agccgataat cgccgcaacg agtaagtaca tcacctggct cagatagagc gccagatgaa
      541 gatttcccat attgattgtg aggattggaa cctcacccag gaccgtaaac atattttcct
      601 ccttcactaa acgcttttcg catatctgac cagctctcgc gacaacagtt ccgttacttg
      661 aaacatggtc atgcgctcgt tctttcggta gacaccaaat gtcactactg ataaacacga
      721 gcgataaaag gaaagggttt cggatggaag gagggctaat gaggttacat cggtgggcgt
      781 ttgctccatt cctcaacggc tttacgccgg gtggcagcgt cccttatggg gcgggaatca
      841 tcgatagtgg ccagcaggtt gcccaactgg atcaggctat ccaggttatg cgctggtaaa
      901 aagttgtcga tatacggtag ggcggtcttc atgccgatgg tcagggggcg gtagtcggga
      961 gagccgagca gcgggttgag ccagatcagg cggtgacaac tgtgctgcag gcgatccatc
     1021 tcaacctcca acatgcccgg ttcgccgcga tcccagccgt cgctgataat cagcacgacc
     1081 gcgccccggc caagcacgcg ccttgcccat ttgtggttga agaaatgcag ggcatcgccg
     1141 atgcgcgtgc cgcccgacca gtcctgcacg gatttcgaga catcgcgcac ggcgtcatcc
     1201 acgtcgcgct tcttgagctg gcgcgtgacg cgcgtcaggc gcgtgccgaa gacgaaggct
     1261 tccacattga gcaggccgtt cgagatggta tggatgaaat gcagcagcag gcgcgaatag
     1321 agactcatcg agccgctgat gtcgcagatg atgacgagcg ggcgcggctt gcgcttcgtc
     1381 tcgcgccagg tcagttcgag cagttccgcg ccgtatttca gattgcggcg cacgatgcgg
     1441 cgcatgtcgg gatacgaacc cctccgcgaa gggcgtttgc ggcgcgttgg acgcaggccc
     1501 agatgccagc gcatctcggc catcagcttc ttggcctcct gcacttcatc ccagctgaac
     1561 gattcaaaat ccttcttgcg cagggcttcg atagcgctgt aggccagtcc ctgcggcgtg
     1621 gcagtatcgt cgtcgctctc ctcgctgcgg cgctcgtcgt cgcggcggcg gcgttcgctc
     1681 tcgcgcatct cggcgcgcag atccttgcgc tgctcctgtg gcgcgttcat atgttcggcc
     1741 aggcgtttga gttcagatgg aggcagacgc aggcgctttt cgtcgcgcct ggccgttccc
     1801 ggcgcttcgg gctttttctt gccgagctgg tcgcgcatat gccagtagta catgtacatc
     1861 tggtcgaaga gcggctcctg ctcgtgtttc gccagcaggc tgcacttgag ggcgttgtaa
     1921 aaatcctcgc ggtacgtcag atcgacaaag gcaagcgtct cggccaggtc gagcattttg
     1981 cgcggcccga catcgatgcc cacttcccac agaatgcgcc caaactccgt aaggcgatac
     2041 agcagccgct cgccgcgctc caattcaaac ggcttgagcg gctccagcgc gaagatatcg
     2101 ccatcctggt tatcgggtga gttttcgcct gggagagcca ccattaagag actccctgcg
     2161 cttttttcag gtagcgagta aattcgctgc cacctatttt cagcacgtca tcctggtatt
     2221 tcagcagcgc ccccagcgtg tcacgcacag gaccatcgac caggtcgatc tggtcaagcg
     2281 ccagcagcga actgatccag tccagcgttt cggcgacgcc tggagctttg tacaggtcag
     2341 cgctgcgcag ctcgcgcaca aaggcgcaga cctgtttcgc cagtcgctca ggcacacccg
     2401 g
//
LOCUS       DASZAK010000549         4073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_405034, whole genome shotgun sequence.
ACCESSION   DASZAK010000549 DASZAK010000000
VERSION     DASZAK010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405034"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..697
                     /locus_tag="VGT44_09655"
     CDS             323..697
                     /locus_tag="VGT44_09655"
                     /inference="COORDINATES: protein motif:HMM:NF020839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2581106.1"
                     /translation="MRISEVSRRTGVSIRSLRYYEQKRLLCARRMENGYRDLDEEAVE
                     RVQTIQMYLGLGLSTEQIEEILQCAGSAPLPQPLPDCEEALLELYQDKLQEVEHQVRV
                     LTALQTRLTERITSLQRQLQSE"
     gene            775..1134
                     /locus_tag="VGT44_09660"
     CDS             775..1134
                     /locus_tag="VGT44_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV2581107.1"
                     /translation="MFVQMQAFGSVEREPELHYTKEGLPHTDFSLAVNTRRCVQDTTI
                     WLYCYAWGDQAETITRSVNKGAMLFVQGDFTSHQYTTEEGKQETRLEVNVRKFSFADE
                     SKPIEVTRSAVDLPRKG"
     gene            complement(1105..3066)
                     /locus_tag="VGT44_09665"
     CDS             complement(1105..3066)
                     /locus_tag="VGT44_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 9 protein"
                     /protein_id="HEV2581108.1"
                     /translation="MNDRPVARFQGRLSVPSLSSVAMSFIVIVALALMFAPRPVKAAG
                     SAFVRVNQVGYATTATKRAYLMASGVETGATFSVKNDDGTIVYSAAIGANLGSWSSSY
                     PDVYALDFGSVTRTGTYTISVSGPIVGFSPNFPIGPAAQLYALALSNSLSFYQNERDG
                     ANYIPSALRTAPGHLNDAHALTYLTPQFDSNDNIIGDLTPLGINIDASGAWWDAGDYL
                     KFVETTGYTVDMMELGVRDFTDQLGSGSSNANFTAEAKFGLDWLQHMWNDTTSTLYYQ
                     VGIGSGNNNIISDHDIWRLPQVDDNYGGKDPLYRYIRNRPVFRAAPAGSLISPNLAGR
                     LAGDFALCYQIFQESKPDYANRCLLSAEHIFDLANTHPKGNLLTSAPYDAYPEVEWRD
                     DLELGATELYYALAHGDLPAGLPHTDAMFYLKSAAHWAHNYITGPNDAADTLNLYDVS
                     GVAHFELYRAITRAGNPTGLEVTRQNLLNDLKKQLDKAIAQAGTDPFGFGYPWATFDT
                     TSHGAGLAVMASEYDELTDSNAYATYGVRWLANILGANAWGTSLIVGDGSTFPDCMQH
                     QVTNLVGSLDGSPPVLAGAAVEGPNSFAATGVVDGMKTCPVNGVDQFAQFNNGSAVYQ
                     DNVQSWSTVEPALDLTATSPLAFAWQING"
     gene            complement(3102..>4073)
                     /locus_tag="VGT44_09670"
     CDS             complement(3102..>4073)
                     /locus_tag="VGT44_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581109.1"
                     /translation="DGTILGAGRGGCGDIYNAPMEGTPWPDSASAAIANIEAAVEAAL
                     RSAQIEASDIETAVFSMAGADWPEDFALLQAAMRVRGYGRKLIVQNDALGVLHAGVAD
                     NVGVSVVCGTGGATGARGPDGRTWHSSAWQDQVQGSSMLSRKAVDAVVRSELGIDPPT
                     SLTRLFLEYFGLESVEEMLHLQTGRGLHLPKRLDGLTRFLLDEAEAGDEVAKNVVLEH
                     GQGLGNYAVVGARYVSLEGTPFTLVLAGGVLRHPSTLLADTIIARVRSTSPDVRPIRC
                     RFEPVIGVLFSALEAASVTVDDALLHRLNPTIPAPDLFATAHDFTLR"
BASE COUNT          946 a         1151 c         1146 g          830 t
ORIGIN      
        1 gggacgattt gcatagtctg ggtcagttgt atgatgctca tagtggttgc cttatctctt
       61 cacaatgacc tgtgaaggta cctctgtttt ccttctatct attctttttt ttgccgcctg
      121 atacggcaaa actccgcaat agtgtacaat gtacattgaa atcttgttct aattatacca
      181 tattgcacaa gccgcgtcaa atttttttca tgagtaaacg acgatgtttg ttgggtccga
      241 atgtggtgcc gcaaactctt gactctgaca ctgatgacat agtttatact gatggtaaac
      301 atgcaggaaa ggagatggac ccatgcgtat tagcgaagta tcccgccgca caggagtgag
      361 catacgctct ctgcgctact acgaacagaa acggctgctg tgcgctcgtc gaatggagaa
      421 cggctatcgc gatcttgatg aggaagccgt cgaacgtgtg caaacgatcc agatgtacct
      481 gggtcttggc ctctccaccg agcagatcga agagattctg caatgcgccg ggagtgctcc
      541 tctaccacaa ccgctgccgg actgcgaaga ggcgctgctg gaactctatc aagacaaact
      601 ccaggaagtc gaacaccaag taagggtgct aacagcgctc caaactcgtc tcaccgaacg
      661 catcaccagt ctccagagac aactacagtc agaataaggt tgaaaggcac tcatgttgcc
      721 tgacatgatt cgatgaaagg ccagcgcggc cttatgcgcc aaaggagcca ttccatgttt
      781 gttcagatgc aagctttcgg cagcgtggaa cgagaaccag agttacacta cacaaaagag
      841 ggcctgccgc atactgattt ctcgcttgca gtcaacaccc ggcgttgcgt gcaagacacc
      901 accatctggc tctactgtta cgcctggggc gaccaggccg aaaccattac gcgctctgtc
      961 aacaaaggcg cgatgttgtt cgtgcaaggc gatttcacct cacaccagta caccaccgaa
     1021 gagggcaagc aggaaacgag acttgaagtc aatgtcagaa agttctcctt tgccgatgag
     1081 agcaaaccaa tcgaagtaac gcgctcagcc gttgatctgc cacgcaaagg ctaatggcga
     1141 agtggcggtg agatcgaggg caggctcaac ggttgaccag gactggacat tatcctgata
     1201 cactgccgaa ccattgttga attgcgcgaa ttgatcaacg ccgttcaccg gacaggtttt
     1261 catgccatcg actaccccgg ttgcggcaaa gctgttgggt ccctctacgg cagcgcccgc
     1321 aagcaccggc ggcgagccat ccagcgagcc aacgagattc gtgacctggt gctgcatgca
     1381 gtccgggaag gtcgagccat cgccaacgat gagcgaggtc ccccaggcgt tggcccccag
     1441 gatgttggcc agccagcgca caccgtaggt cgcataggcg ttgctgtcgg tgagttcatc
     1501 gtactcgctt gccatgacgg ccagaccggc cccgtgggag gttgtatcga aggttgccca
     1561 ggggtaacca aaaccgaaag gatcggtccc agcctgggcg atcgccttat ccaactgctt
     1621 cttcaaatca ttgaggagat tctgccgggt cacttcgagt cccgttggat tccctgctcg
     1681 cgtgattgca cggtagagct cgaaatgagc aactccgctc acatcgtaaa ggttcagggt
     1741 gtcggctgcg tcgttgggtc cggttatata attgtgggcc cagtgggcag cagatttcag
     1801 atagaacata gcatcggtgt gcggcaaacc ggcgggcaga tcgccatgcg ccagggcata
     1861 atacagctcc gtcgcgccca actccaggtc atcgcgccac tcgacctccg gataagcatc
     1921 gtaaggagcg gatgtgagca gattaccttt ggggtgagtg ttcgccaggt caaagatatg
     1981 ctccgccgaa aggaggcagc ggttcgcgta gtccggtttg ctctcctgga aaatctggta
     2041 gcacaacgcg aagtcgccag ccagtcgccc ggcaagattg ggactgatca gcgatccggc
     2101 gggggcagcg cgaaagactg gacgattacg aatgtagcgg tagagtgggt ctttcccacc
     2161 gtagttgtcg tccacctgcg gcagacgcca gatatcgtga tcgcttatga tgttgttgtt
     2221 tcccgaaccg atgccaacct gatagtaaag cgtcgaagta gtgtcattcc acatgtgctg
     2281 gagccaatcc aggccgaact tcgcctcggc ggtaaaattc gcattggatg aaccagagcc
     2341 aagctgatcg gtgaagtcac gcacaccgag ttccatcata tcgaccgtgt agccggtagt
     2401 ctcaacgaat ttcaagtaat cgccagcatc ccaccaggcc cctgatgcat cgatattgat
     2461 gcccagcggg gtgagatcgc caatgatgtt gtcgttgcta tcaaattgag gagtgagata
     2521 ggtcaaggcg tgcgcgtcat tgagatgtcc tggagcggtg cgcaaggccg atggaatata
     2581 gttggctcca tcgcgttcat tctgatagaa agagagcgag ttagataacg ccagggcgta
     2641 cagctgcgcg gcgggaccga ttggaaagtt cggcgagaag cccacaatcg gcccgctcac
     2701 tgagatcgtg taggtaccgg tcctggttac tgagccgaag tccagggcat agacatcggg
     2761 atagctgcta ctccacgaac caagatttgc cccgatcgca gccgaataaa cgatagttcc
     2821 atcgtcattc ttgacgctaa atgtcgcgcc ggtctccacg ccggaggcca tgagatacgc
     2881 tcgtttcgtc gcggtggtcg cataccccac ctgattgacg cggacgaagg ccgaaccggc
     2941 ggccttgacc ggtcgcggcg caaacatcag ggccagagct acaataacga tgaaactcat
     3001 ggcgacactt gagagagatg gaacggagag acgaccttga aacctggcca ctggacggtc
     3061 attcatggtt ttcctccttg aaaaaaatat gaacaggcag tctatctcag ggtaaaatca
     3121 tgcgcggtag cgaacaggtc cggcgcgggg atggtcggat tgagccggtg cagcagcgcg
     3181 tcatcgacgg taacacttgc ggcctccaat gccgagaaaa ggacgccgat caccggctcg
     3241 aagcggcagc gtatcgggcg tacatcgggc gaagtagagc gcactcgtgc gatgatcgta
     3301 tcggccagca gtgtcgaggg atggcgcagg acgcccccag ccagcacaag cgtgaagggc
     3361 gtgccttcca aactgacata gcgcgcaccc acaaccgcgt agtttcccag gccctgacca
     3421 tgttccagaa caacgttctt tgctacctcg tcgcccgcct ctgcttcatc aagcaggaag
     3481 cgcgtcagac catccaggcg ttttgggaga tgcaggccgc gcccggtttg caggtgcagc
     3541 atctcctcga cgctctccag gccgaaatac tcaagaaaga gccgggtgag cgatgtcggc
     3601 gggtcaatgc caagctctga gcgcaccaca gcatccacgg ctttgcggct caacatgcta
     3661 ctaccctgca cctgatcctg ccaggcgctg gaatgccagg tgcgtccatc cggcccgcgc
     3721 gcgccggtcg ccccgcccgt gccgcacaca acggagacac ctacattatc ggctacgccc
     3781 gcgtgcaaca cccccagcgc gtcgttttgc acgatcagtt tgcggccata gccgcgcact
     3841 cgcatggccg cttgcagcag ggcaaaatcc tccggccaat ccgcgcccgc catgctgaat
     3901 acggcggttt cgatgtcgct tgcctcgatt tgcgcggaac gcagggcagc ttcaaccgca
     3961 gcctcgatgt tggcaatcgc cgcactggca gaatcaggcc agggagttcc ctccatagga
     4021 gcattgtaga tatcgccaca gccgccgcga cccgccccca ggatcgtccc gtc
//
LOCUS       DASZAK010000550         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_582140, whole genome shotgun sequence.
ACCESSION   DASZAK010000550 DASZAK010000000
VERSION     DASZAK010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582140"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /gene="hrcA"
                     /locus_tag="VGT44_09675"
     CDS             <1..892
                     /gene="hrcA"
                     /locus_tag="VGT44_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV2581110.1"
                     /translation="LYQPHTSAGRVPTDLGYRYFVEHLMQESALSLDEQRLIRHQFYQ
                     VQDQLDQWVRLTASVMARLLHSAAVMSSPRANVGYLKHFELLSVTDLSAHLVLVLRDG
                     TLKQQRLLLETSIDQEELSALSVRLNRLFQERSASEVEALLSRYDFSPTERLVATTIM
                     HILAQHGDPLGDVFYREGVVNILDQPEYSSMGPEEERNERVRKVMEVLEQNRFLPVLA
                     SQLRESDGVQIIIGGENEWDDMRDVSLVVARYGQEGKIGGLLGVIGPTRMQYGRAIAV
                     VRYMAQVMNELLAEMYGFDE"
     gene            938..>1729
                     /locus_tag="VGT44_09680"
     CDS             938..>1729
                     /locus_tag="VGT44_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEV2581111.1"
                     /translation="MPKVIGIDLGTTNSCVAVMEGGEPVVIPNSEGARTTPSIVAVNK
                     SGERLVGEVAKRQAVTNPENTVYSIKRFMGRKYDDVEVARDRGLVPYKVVKAPNGDAW
                     VEIQGKQYSPPEVSAMILQKMKTDAEAYLGERVLQAVITVPAYFNDAQRQATRDAGKI
                     AGLDVLRIINEPTAASLAYGLDKRKDEKIAVYDLGGGTFDISLLELGDGVFEVKSTNG
                     DTHLGGDDFDQRIINWLADEFRRESGIDLRGDRMALQRLKEAAEKA"
BASE COUNT          374 a          451 c          563 g          341 t
ORIGIN      
        1 tctctaccag ccgcatacat cggcggggcg cgtgccgacc gacctgggtt atcgctactt
       61 tgtagaacat ctcatgcagg aatcggcgct ctcgcttgat gaacagcgcc tgatccggca
      121 ccagttctac caggtgcagg accagctcga tcaatgggtg cgcctgaccg cgtcggtcat
      181 ggcgcggctg ttgcatagcg ccgccgtgat gagctctcca cgcgccaatg tgggttatct
      241 caagcacttc gagttgctat cggtgaccga tttgtcggcg catctcgtgc tggtgctgcg
      301 tgatggcacg ctcaaacagc agcgtctgct gctcgaaacg tctatcgatc aggaggagct
      361 gagcgcgctt tcggtgcggc tcaacagact tttccaggaa agaagcgcga gcgaggtaga
      421 ggcgctgttg agccgctacg atttttcgcc gaccgagcgg ttggtcgcga ctaccatcat
      481 gcatattctg gcgcaacatg gcgaccccct gggcgatgtg ttctatcgcg aaggcgtggt
      541 caacatcctg gatcagccgg agtatagcag catggggccg gaggaggaac gcaacgagcg
      601 tgtgcgcaag gtcatggagg tgctggagca gaatcgtttc ctgcccgtcc tggcgtcgca
      661 gttgcgcgag tcggatggcg tacaaatcat catcggaggc gagaacgagt gggacgatat
      721 gcgtgatgtc agcctggtgg tggcgcgcta cggacaggaa ggcaagatcg gcggtctgct
      781 aggggtgatt ggcccgaccc gtatgcaata tgggcgagcg attgccgttg tgcgttacat
      841 ggcgcaagtc atgaacgagc tactggcgga aatgtacggt ttcgatgaat aacaccattt
      901 tgggaacaca tatacaacaa caaataggag gatacctgtg ccaaaggtta ttggaattga
      961 tttaggaaca accaattcgt gtgtcgcagt gatggaaggc ggagagccgg tcgtcattcc
     1021 gaactcggaa ggcgcgcgca ctacgccctc aatcgtggcg gtcaacaaga gcggtgagcg
     1081 cctggtgggt gaagtcgcca agcgccaggc cgttactaat ccagaaaata cggtttactc
     1141 gatcaagcgg tttatgggac gcaagtacga cgatgtcgag gtggcgcggg atcgcggcct
     1201 ggtgccatac aaagtggtca aggcccccaa cggcgatgcc tgggtggaga ttcagggtaa
     1261 gcagtacagc ccgccagagg tgtcggccat gatcttgcag aagatgaaga cggatgccga
     1321 agcgtacctg ggcgagcgcg ttcttcaggc cgtgattacg gtgccggcct acttcaacga
     1381 tgcccagcgc caggccacac gcgacgctgg caagatcgcc gggctggatg tgctgcgcat
     1441 catcaacgag ccaaccgccg cgtcgctggc gtatgggttg gataaaagga aagatgaaaa
     1501 gatcgctgtc tacgacctgg gaggcggcac cttcgacatt tcgctgctcg aactgggcga
     1561 tggcgtcttt gaggtcaaga gtaccaacgg cgatacgcac ctgggcggcg acgattttga
     1621 ccagcgcatc atcaactggc ttgccgacga gttccgccgc gagagtggca tcgacctgcg
     1681 tggcgaccgc atggccttac agcgtttgaa ggaggcggcg gagaaggcc
//
LOCUS       DASZAK010000551         1941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_369522, whole genome shotgun sequence.
ACCESSION   DASZAK010000551 DASZAK010000000
VERSION     DASZAK010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369522"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VGT44_09685"
     CDS             complement(<1..321)
                     /locus_tag="VGT44_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2581112.1"
                     /translation="MNVMDIFETMEYGPAPEAAAPGLAWIAERSPFGLFINNRWVKPA
                     SGKYIDSINPGNDKLLAKVAAANAKDVDTAVDAARAAFETWGRTPGHARARYLYAIAR
                     HIQKH"
     gene            complement(436..1464)
                     /gene="deoC"
                     /locus_tag="VGT44_09690"
     CDS             complement(436..1464)
                     /gene="deoC"
                     /locus_tag="VGT44_09690"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV2581113.1"
                     /translation="MTTTRKRTEPIDERLAAPTIAPVHAPALARNPGIPLDMDWVRQA
                     RVNRSAVERRAITIPTRRTVKKEWQAAWLLRAITCIDLTTLQGDDTPGNVLRLCAKAR
                     RPVRQEVLEALGAGKMDLKVGAVCVYHALVEVAVEALRGSDIPVAAVSTGFPAGQISF
                     EQKLQEIAASIKAGAAEIDIVISRAHVLTGDWQALYDEVRAFREACGQAHMKTILATH
                     ELATLRNVDMASLVCMMAGADFIKTSTGKEPVNATLPVGVVMTRAIRDYRERTGYMVG
                     FKPAGGIRTAKQSLDWLILMKEELGNAWLSNTLFRIGASSLLSDIERQLSYYVTGHYA
                     AAHHNPMV"
BASE COUNT          383 a          580 c          567 g          411 t
ORIGIN      
        1 gtgcttctgg atgtggcgcg cgatggcgta cagatagcgc gcgcgcgcgt ggccgggcgt
       61 cctgccccac gtctcgaagg cggcgcgggc agcatcgacc gccgtgtcta cgtctttggc
      121 attggcggcg gcaactttcg ccagcagctt gtcgttgccg ggattgatgc tgtcgatata
      181 cttgccgctg gcaggcttga cccatcgatt gttgatgaac aggccgaaag gcgagcgttc
      241 cgcaatccag gccaaccctg gcgcggctgc ttccggcgcg ggcccgtact ccatcgtttc
      301 aaagatatcc ataacgttca tctctcacct cccttactgc aattacgctt gaccttttag
      361 gacttgacgt gtatactttc ccttcggagc ggcaggaaca atgtcctttt acgacccgct
      421 tgagggtact gcaacttaaa ccatcggatt gtgatgcgcc gccgcgtaat gaccggtgac
      481 gtagtacgaa agctgtcgtt cgatatcgga gagcaggctg ctggccccga tgcggaaaag
      541 cgtgttggag agccaggcgt tgccgagttc ttccttcatc aagatcagcc agtcaagcga
      601 ttgtttggcc gtgcggatac cccccgccgg tttgaagccg accatgtagc cggtgcgctc
      661 cctgtaatca cgaatggcgc gggtcataac gacgccaacg ggcagcgtgg cgttgacggg
      721 ctctttgccg gtagaggtct tgatgaagtc cgcgccggcc atcatgcaga ccaggctggc
      781 catatcgacg ttgcgcagtg tcgccagttc atgggtcgcc aggatggtct tcatatgcgc
      841 ctggccgcaa gcctcgcgga aggcgcgcac ctcgtcatag agcgcctgcc agtcgcccgt
      901 cagcacgtgc gcgcgcgaaa taacgatatc gatctcagca gccccggctt tgatcgatgc
      961 cgcgatctcc tgcaacttct gctcgaagga aatctgtccg gcggggaagc cagtcgagac
     1021 ggcagccacg ggaatgtccg acccgcgcag cgcttcgaca gccacctcta ccagcgcatg
     1081 atagacgcag accgctccca ccttgaggtc catcttgccc gcgcccagcg cctccagcac
     1141 ctcctggcgc accggcctgc gcgccttggc gcacagccgc agcacgttgc caggcgtgtc
     1201 gtctccctgc aaagtcgtca ggtcgatgca agtgatggcg cgcagcagcc acgccgcctg
     1261 ccactccttc ttaacggtgc gccgcgtcgg gatggtgatt gcgcgccgct caaccgcgct
     1321 gcgattgacg cgcgcctgcc gcacccaatc catgtcgagc gggatgccag ggtttcgcgc
     1381 cagcgcgggc gcgtgaacgg gcgcaatggt gggagcagcg agacgttcat caataggttc
     1441 agtgcgtttt cgggtggtgg tcatatggtt cgtcctttgg gtctgcctgg tagcgaagca
     1501 atttgctgta aatatgcctt ctacataaat cttggctcat aagatggata atgtcaagct
     1561 ggatgaagcg tcagtccctc tatcaaacat cgtctcaccg gctcgaaatt tacggaactc
     1621 gtcaggcatt ccttctggca tcgcgcgaca aggacgttcc acccatgaca tttgtcatgg
     1681 tcaaatcagg agagatttca cttccgacga gttcttccct gggctatact ctcatgcgaa
     1741 agcacacatt gccaaaagga taggtaccat cccatggctg ctgcgggcct ctgcaaggga
     1801 attccccgag ttgagctccg gcctgcttct ggaacaggcg caggtacctg agctatgaga
     1861 agcgcattca tacaaggaga atacctcatg aaacgttttc gacaggaacg agagttgaag
     1921 ccggatgcag aagaagatgc c
//
LOCUS       DASZAK010000552         1531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_297022, whole genome shotgun sequence.
ACCESSION   DASZAK010000552 DASZAK010000000
VERSION     DASZAK010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297022"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..>1531)
                     /locus_tag="VGT44_09695"
     CDS             complement(192..>1531)
                     /locus_tag="VGT44_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2581114.1"
                     /translation="LLVGLPGTGKTLLAKAVAGEAGVACFNVSASAFVEMYVGVGAKR
                     VRDLFHLARENAPCVIIIDELDAVGRKRTMSLGGSDERDQTLNQLLVELDGFAARQTI
                     VVLAATNRADILDPALLRPGRFDRHINVSLPDRAGRQAILRVHTRHTPLHDEVSLEHL
                     ARLTTGMSGADLANLVNEAALCAARRDLERITSACFEEALARVLLGAQRPIVLSDTER
                     RIIAFHEAGHALVAHHLPEADCVSRVTILPRGQSLGVTQFVAEEDRYNYSRESLMARL
                     TMGLGGRAAEELTFGPDQVTTGAENDFQVVTDLARKMVTRWGMSERMGMMFVEGWTAA
                     SGPALRYHNGEALSTHSRTLVADATGRLLLNGGDLPARQQRFGTLEAAASEASSASMA
                     TLIDQEVQNLLSEGYQTAKTLLSEHSDQLNKLAQALMEHEQLDRAAFEQLLSA"
BASE COUNT          289 a          484 c          435 g          323 t
ORIGIN      
        1 gttgctttta tctcgcccct tgaaagggga gcggttacat acgtaacttc atgaatagcg
       61 tacgtgatga gtgtgaagaa aagcgttggc aggggcatta aaaaatgtaa agcctctcta
      121 tgcacctgat actggtttcc ctgatcgcca tattgagacc tgtagagcat cagcacccat
      181 accctggtct ctcacgcgct tagcagttgc tcaaaagcgg cacgatccag ttgctcgtgt
      241 tccatgagcg cctgagccag cttgttgagc tgatcggagt gctcgctcaa cagagtcttc
      301 gctgtctgat agccctcgct gagcaggttc tgtacttcct ggtcgattaa tgttgccatg
      361 ctcgcgctgc tcgcttcact ggctgcagcc tccagcgtcc caaatcgctg ttgacgtgca
      421 ggcaggtcac caccattcag cagcagtcgc cccgttgcat cagcgacgag cgtccgactg
      481 tgcgtggaaa gcgcttcgcc attgtggtaa cgcaaggccg ggccgcttgc ggctgtccag
      541 ccctcgacga acatcatgcc catgcgctcg ctcatccccc agcgtgtgac catctttctg
      601 gcgaggtcgg tcaccacctg gaaatcgttc tccgcgccgg tggttacttg atctggccca
      661 aaagtcagtt cttctgccgc ccggcctccc aggcccatgg tcaggcgagc cataagactt
      721 tcccgactat agttataacg gtcctcctcg gccacgaact gcgtcactcc caggctctgc
      781 ccacgcggca ggatggttac tctactgacg cagtcagcct ccggcaggtg gtgggctacc
      841 aatgcatgcc ccgcctcgtg aaaggcgatg atacgccgtt ccgtgtcgct cagcacgatg
      901 gggcgttgcg ccccaaggag cacacgcgcc agggcctcct cgaagcacgc gctggtgatg
      961 cgctccaggt cgcgacgggc agcacatagc gcggcttcat tgacgagatt ggccaggtcc
     1021 gcgccactca tccccgtggt gagccgtgcc aggtgttcca ggctcacctc atcgtgcagt
     1081 ggagtgtgcc gtgtatgcac ccgtaggatg gcctggcggc ccgcccggtc tggcagcgag
     1141 acgttgatgt gacggtcaaa gcggcccgga cgcaacagag ccggatccag gatatcagca
     1201 cggttggtcg ccgccagcac gacaatagtt tgccgtgctg cgaagccgtc gagttcgacc
     1261 aggagctggt tgagcgtctg gtctcgttcg tcactgccgc cgaggctcat tgttcgtttg
     1321 cgacccaccg catccagttc atctatgatg atgacgcagg gagcgttctc ccgcgccagg
     1381 tgaaacaggt cacgcacgcg ctttgccccc accccgacgt acatttcgac aaacgcagaa
     1441 gcgctcacat tgaagcacgc cacccccgct tcaccggcga cggctttggc gagcagggtt
     1501 ttgcccgtgc caggaagacc caccagcaat g
//
LOCUS       DASZAK010000553         3069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_226946, whole genome shotgun sequence.
ACCESSION   DASZAK010000553 DASZAK010000000
VERSION     DASZAK010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226946"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VGT44_09700"
     CDS             complement(<1..273)
                     /locus_tag="VGT44_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEV2581115.1"
                     /translation="MTNQSNEPQQLTHLDESGNIHMVDVTDRAETSREAVAKARVRLL
                     PETVRLIESNQVSKGSVLEVAKIAGIMAAKQTPQIIPLCHPLPMTHI"
     gene            complement(284..745)
                     /gene="ndk"
                     /locus_tag="VGT44_09705"
     CDS             complement(284..745)
                     /gene="ndk"
                     /locus_tag="VGT44_09705"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV2581116.1"
                     /translation="MERTLVLIKPDAIQRDLVGEIIQRLERKGLNLVGLKMIALSDAL
                     LDEHYSHLAGRDFFGEIKTFMRSTPVIACCWEGVDCIDTVRALCGITKAREAAPGTIR
                     GDLAMSVQANLVHASDSLETASVEVERFFAPEELFGYEDVLNPFIYNSREK"
     gene            878..1702
                     /locus_tag="VGT44_09710"
     CDS             878..1702
                     /locus_tag="VGT44_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581117.1"
                     /translation="MSKPIPQHQLNTDSGVRSGAAQPQYYAMQAIKKVPEITALFWII
                     KLLSTAMGESTSDYLVYQINPYVAVVLGCLGLAVALILQLQARRYVAWIYWLAVVMVA
                     VFGTMAADVAHVVLGIPYLVSTAFFASALAIIFVIWYSSEKTLSIHTVYRGRRELFYW
                     ATVMATFALGTAAGDLTASTLRFGYFPSVVLFAVLFALPALAYWLFGLNEIVAFWFAY
                     VVTRPLGASVADWLGKPYLGGLGLGDAKVALVLTVLIIGFVGYLTITHKEMKGEER"
     gene            1743..3005
                     /locus_tag="VGT44_09715"
     CDS             1743..3005
                     /locus_tag="VGT44_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV2581118.1"
                     /translation="MHTQNQQKRVLLLMSPATYRAGAFLSAAKKLNLAVVVGVDMPET
                     LTEYWHVPLGLDFAEAQRSIETIAEYAKEHPFTAILSVDDSATELAARASAALGLAHN
                     SPQAAEAARNKLLMRTLMSTGGAPCPVFRPFFLGDDPAWIAGQIAYPCVLKPLRLSGS
                     RGVMRADNPSEFVTVFQRLKRMLLSEGNSEHETAFLVEDFIPGYEVALEGLLTGGELK
                     VLALFDKPDPLDGPFFEETIYVTPSRLPQETQDAVARCVSIAAASLGLREGPVHAELR
                     VNERGPWMLEMAGRSIGGLCSTVLEFGSGMCLEELILRHATGEVIASVERESGAAGVM
                     MIPIPSAGILKAVHGVEEALQVPLVTGVEITAKLNYALVPLPEGASYLGFIFARGQTP
                     AEVEAAIRQAHGQLRFEIRREIPVLRAG"
BASE COUNT          591 a          859 c          906 g          713 t
ORIGIN      
        1 gatgtgggtc atgggcagcg ggtggcagag cgggatgatt tgcggcgtct gcttggcggc
       61 cataatgccg gcgatcttgg ccacttccag cacactgccc ttcgagacct gattagattc
      121 gatcaaccgc accgtctccg gcagcagacg cacacgcgcc tttgcgacgg cttcgcgaga
      181 cgtctccgct ctatcggtca catccaccat atgaatgttg ccgctttcat caaggtgcgt
      241 caactgctgc ggttcattgg attggttggt catagaattg gctttacttt tctcgcgagt
      301 tgtaaataaa tgggttcaac acgtcttcgt agccaaatag ttcttcgggt gcgaagaaac
      361 gctccacttc gacgctggcc gtctcaagcg agtcggaggc atgtacgagg ttggcctgta
      421 cgctcatcgc caggtcgccg cgaatggtgc cgggcgcggc ctcgcgagcc ttcgtgatgc
      481 cgcacaacgc gcgcaccgta tcgatgcagt cgacgccttc ccagcagcag gcgatcaccg
      541 gtgtactgcg catgaacgtc ttgatctcgc caaagaaatc gcgcccggcc aggtggctgt
      601 aatgttcatc caggagtgca tcgcttagcg cgatcatctt caaaccgacc aggttgagtc
      661 ccttgcgctc caaccgctga ataatttccc cgacgagatc gcgctggatc gcgtcaggct
      721 ttattagcac cagagttcgt tccatgtgta gaaatatccc tttcgtatag tcaatgacgt
      781 gactggtgat gtgttgtctg cattattgta acgtgaatct ggggaaatgc aagaccttgc
      841 cgcctatgtt atactgctaa gaaatatgag ggagaagatg agtaaaccca taccacagca
      901 ccaactcaac actgattccg gcgtccgtag cggagccgct caacctcagt attacgccat
      961 gcaggctata aaaaaggttc cggaaattac cgccttattc tggatcataa aattgctgag
     1021 taccgcgatg ggcgagtcta cgtcggacta cctggtgtac cagattaacc cctacgttgc
     1081 ggtcgttctc ggatgtctgg gcctggctgt tgcattgatc ttgcaactcc aggcgcgtcg
     1141 ctatgtcgcg tggatttact ggttagcggt cgtcatggtg gccgtcttcg gcaccatggc
     1201 cgccgatgtc gcacacgtgg tgctaggtat tccatatctg gtatcaacgg ccttctttgc
     1261 ctccgcactc gccattatct tcgtgatctg gtatagcagc gagaaaacgc tctccatcca
     1321 tacggtctac cgtgggcgcc gcgaactctt ttattgggcg acggtcatgg cgacgtttgc
     1381 gcttggcacc gctgcgggcg acctgaccgc ctcaaccctg cgcttcggtt actttccatc
     1441 ggtagtcctc tttgcggttt tgttcgctct cccggctctg gcgtactggt tgtttggtct
     1501 gaacgagatc gtggccttct ggtttgccta cgttgtgacc cggccgctcg gcgcttcggt
     1561 tgccgactgg ctggggaaac cgtatctggg tggtctcgga ctgggtgacg caaaggtggc
     1621 cctggttttg acggtgttga ttatcggctt cgtcggctac ctgacaatca cccataagga
     1681 aatgaagggc gaagaacggt agctgatgcc atttttgtag agtgataacg atgtcgcaac
     1741 ccatgcacac acaaaaccaa cagaagcgcg tcttactgct gatgtcgccc gcaacctatc
     1801 gcgcgggggc atttttgagc gcggcgaaaa agctgaatct ggcggtcgtg gtcggggtgg
     1861 atatgccaga gacgctcacg gaatactggc acgtgccgct ggggctggat tttgccgagg
     1921 cacaacgatc gattgagacg atagcggaat atgccaaaga gcatcctttt accgcaattc
     1981 tttcggttga tgatagcgcg acggaactgg ccgcgcgcgc cagcgccgcg cttggcctgg
     2041 cgcataactc accgcaagca gcagaggccg cgcgcaacaa gctgctgatg cgcacgctca
     2101 tgtccacggg cggcgcgcca tgccccgtct ttcgcccgtt cttcctgggc gacgatccgg
     2161 cctggattgc ggggcagata gcgtatccat gcgtgctgaa gcctctgcgg ctctccggca
     2221 gccggggcgt catgcgcgct gataatccaa gcgaatttgt gacggtattc cagcgcctca
     2281 aacgaatgct tctgtcggaa ggcaactcgg agcatgaaac cgccttcctg gttgaggatt
     2341 ttatccctgg ctacgaggtg gccctggagg gcttgctgac aggaggcgaa ttaaaagtac
     2401 tggccctgtt cgataagccg gacccgctgg atgggccgtt ttttgaggag accatctatg
     2461 tgacgccctc gcgcctgcca caagagaccc aggatgctgt tgcgcgctgc gtctccatcg
     2521 cagcagcctc gcttggcctg cgagagggtc ccgttcacgc cgaactgcgt gtcaatgagc
     2581 gaggaccctg gatgctggag atggcgggtc gttcgattgg cggtctgtgt tcgaccgtac
     2641 tggaatttgg ctccggcatg tgcctggaag agttgattct gcgccacgct accggcgagg
     2701 tgattgcctc ggtcgaacgc gagtcgggcg cagccggggt catgatgatc ccgattccat
     2761 ctgccggtat cctcaaagcg gtgcatggcg tcgaagaagc cttgcaagtt ccactggtga
     2821 cgggcgttga aattaccgcg aagctgaatt atgcgcttgt ccccctgccg gagggcgcga
     2881 gctacctggg cttcatcttc gcgcgcggcc aaacgccagc cgaggtggaa gccgccatac
     2941 ggcaagcgca tgggcagctg cgcttcgaga tccggcggga gataccggtg ctgcgggcgg
     3001 gataattagt caatgcctgg tcatcaatag gaaaccctca ctctccgccg ccttcttgta
     3061 cctggtcgg
//
LOCUS       DASZAK010000554         3656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_119797, whole genome shotgun sequence.
ACCESSION   DASZAK010000554 DASZAK010000000
VERSION     DASZAK010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119797"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VGT44_09720"
     CDS             <1..796
                     /locus_tag="VGT44_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581119.1"
                     /translation="REFIASDERERARLIREGKPLQQQRTWPRAYSVFEDAERGRIYL
                     LMDYIPGLSLDNLRRTLPSPATSQFTPRPPSAGSRIEAMTPIPLPVRRRVAGTKKREK
                     ILFFALALLIGAVFSVSLFMFFIKSPLTHTATPRATSTPRAIPASTLIHRTSFYLVLA
                     TAYAGTVVDLLNDEKTAMYLMGLQQNQGRLSGLFQGLGLAGPIAGTVTSSGHVRFKLS
                     VISNDITLVFDGDIKIGGDIAGSFVALNQLGNNTGESGIWNIAAIP"
     gene            898..3216
                     /locus_tag="VGT44_09725"
     CDS             898..3216
                     /locus_tag="VGT44_09725"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV2581120.1"
                     /translation="MSLIPRFLRPSRSLALPSEPMRFGVATADHQCEAYDPQHEDIRD
                     VWERRRGLTKRGIATDFWNRYPEDVKLARDLGCKVFRFSLAWSRLEPSPGQFDDAAFE
                     HYRQVIETIRAAGMEPLMTLHHFTWPVHVEQRGGLIGKDFPEIYTSYVSEVVKRLGPL
                     VHYWLTFNEPTQLIYGYIKPWWEQYYFCPPGLPEHATFADELDAVGKLMRNLFMAHTA
                     ARKIIKESNPDAQVGVNPMLLGLPGWLQGFIDWNVTRLRTWDDWVRKGVRFGRRRAYE
                     QGKVDIMLATLSITRDRSEQVDFSESYFVAHQALLIPAESQVRSVMDLDGRPVAVIKY
                     STSQKMQGRLLPQTGTLSVGSLVEALQALDTGQAAAFLSDDVILHGLMQEYPERYRLL
                     ESLPHREHYAAAVAKGHRGLMSVINRAIQNFVEKGHWEASVEKHLTGLSTQPPTEVRM
                     SETLSDINNWQSMRPEDAAQTQQLARRTIIGPRSLTRHIQRRGHIIVAVKEDVPGLGY
                     RDPKTGELHGLEIDLARSIAQDLLGDPNKIIFRPIHTGDHLPLVRSTLRRFDTFFKLY
                     SVLSTTLCSNWWHLGMAGRLPTFLCPKECVDQQDFVGFDYYWGIPSIGLHRFQQLLSA
                     VVGHYSDAPVWPGALYSMLKYYARMFPHKELIIIENGSVVKASGIDRATYLERHVSQV
                     ERARRRGIKIPIYICWSITSNREWGLPFGDGNDFGLYHIDLDTDPTLERKCTDSAGIY
                     KRLIAQHSGTVAIPSPTETRNGGTVETPGQHP"
     gene            complement(3259..>3656)
                     /locus_tag="VGT44_09730"
     CDS             complement(3259..>3656)
                     /locus_tag="VGT44_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581121.1"
                     /translation="RSTSLVSLSGGPLSNHFPFEYQNQPLHTPWDLAYISLEATPQEL
                     SRETGQYRFVPDRKSELARPGALMPGAQEHISDLREMEHAMQREQFYRYMIRYWRRFH
                     MLLVLVTIGLTAWHLIYAAQLLIPVLLHR"
BASE COUNT          780 a         1141 c          986 g          749 t
ORIGIN      
        1 tcgagaattc atcgcgtcag atgagcgtga gcgcgctcgc ttgatccgtg aaggcaagcc
       61 acttcagcag cagagaacct ggcctcgtgc ttatagcgtc ttcgaagatg cagaacgtgg
      121 tcggatctat ctgctcatgg actacattcc aggattgagc ctggataacc tacgtaggac
      181 acttccatca cctgctacct cccaattcac acctcggcct ccatccgccg ggtcaagaat
      241 tgaagcgatg acacctatcc ctctaccggt tcgccggcgc gttgctggca cgaaaaaacg
      301 cgaaaaaatt ctcttcttcg cgctcgcgct cctgataggc gcggtcttta gcgtgagtct
      361 cttcatgttc ttcataaaat cgccactcac tcatacagct acgcccaggg ctacatctac
      421 ccctcgggct atccctgctt ccacgctaat ccaccgaaca tccttctatc tcgtgcttgc
      481 cacagcgtac gctggcacgg tggttgatct gcttaacgat gaaaaaacgg caatgtacct
      541 gatgggccta caacaaaacc agggcaggct gagcggtctg ttccagggcc tcggattagc
      601 cggtccaatc gccggaactg tcacctcttc cgggcatgtt cgcttcaaat tgagcgttat
      661 atcgaatgat atcactctgg tttttgacgg agatatcaaa ataggaggag atatagcggg
      721 aagctttgtc gccctgaacc agctaggaaa caacaccggg gaatctggca tctggaatat
      781 tgcggcaatc ccctgatcgc cccatcccct cactgcttgc gcagaaccac tcagcatggt
      841 agagtatgtc caattataca gtgcaaaccc gtattatatt gtcgaaggag gttgccaatg
      901 tcgcttatac ctcgctttct tcgtccctcg cgttcactcg cactgccgtc tgaaccgatg
      961 cgtttcggcg tggctaccgc cgatcatcag tgtgaagcct atgatccgca gcatgaggat
     1021 atccgcgatg tatgggaacg gcggcgcggc ttgacgaagc gcggcatagc caccgacttc
     1081 tggaatcgct accccgaaga tgtgaaactg gcgcgcgacc tcggctgcaa ggtctttcgc
     1141 ttctcgctgg cctggtcgcg gctggaaccc tcgccgggtc agttcgatga cgcggccttc
     1201 gagcattacc ggcaggttat cgaaaccatc cgcgcggctg gcatggagcc gctcatgacg
     1261 ctgcaccact tcacgtggcc ggtgcatgtc gagcagcgcg gcggcctgat cggcaaggac
     1321 ttccccgaaa tctataccag ctacgtctcc gaggtcgtca aacgcctggg gcctttagtg
     1381 cattactggc tcacatttaa cgagccgacg cagctgatct acggctacat caaaccctgg
     1441 tgggagcaat attacttctg tcctcccgga ctaccggaac atgccacctt tgccgacgaa
     1501 ctggatgccg tcggcaaatt gatgcgcaac cttttcatgg cgcataccgc cgcgcgcaag
     1561 atcatcaaag agagcaaccc ggatgcgcag gttggcgtca atcccatgct gctcggcctg
     1621 cccggctggc tgcaaggctt catcgactgg aacgtcacgc gcctgcgcac ctgggacgac
     1681 tgggttcgca aaggcgtccg ctttggccgc cgcagggcct atgagcaagg caaggttgat
     1741 atcatgcttg cgacactttc tatcacccgt gatcgctccg agcaggtcga tttctccgag
     1801 agctactttg tcgcgcacca ggcgctgctt attccggcgg agagccaggt gcgatccgtc
     1861 atggacctgg atggccgccc tgtcgctgtc ataaaatatt ccacgtccca gaaaatgcag
     1921 ggaagactcc taccgcagac cggtacgctg tctgtgggca gtctcgttga ggcattgcag
     1981 gcgctcgata ccgggcaggc tgcggcattt ctatcggatg atgtcatcct gcatggcctg
     2041 atgcaggagt atcctgaacg ctatcgcctg ctcgagtcgc tgccccaccg tgaacattac
     2101 gccgccgccg tcgccaaagg ccacaggggg ctgatgtcgg tcatcaatcg cgccatccaa
     2161 aacttcgttg agaagggcca ctgggaggcc agcgttgaga agcatttaac tggcttatcg
     2221 acgcagcccc caaccgaggt gcgcatgtcc gaaacgctgt ctgacatcaa caactggcag
     2281 tcaatgcgcc cggaagatgc ggcgcagaca cagcagcttg ctcgcaggac aatcatcggg
     2341 ccgcgctcgt tgacacggca tatccagcgg cgcggccaca tcatcgtagc tgtcaaggaa
     2401 gacgttcccg gcctgggcta tcgcgacccc aaaaccggag aactacacgg ccttgagatt
     2461 gacctggcgc gctccatcgc gcaggatctg ctcggcgatc cgaacaagat catctttcgc
     2521 cccatccata ccggcgatca ccttcccctc gtgcgttcga ccttgcgccg tttcgatacc
     2581 ttcttcaagc tctacagcgt cctctctacc accctttgca gcaactggtg gcacctgggc
     2641 atggcaggca ggctgccaac ctttttatgc cccaaagagt gcgtagacca gcaggacttc
     2701 gtcggcttcg actactactg gggcatccca tccataggac tgcatcgctt ccagcaactc
     2761 ctctcggcag tggtcggcca ctacagcgac gcccccgtct ggcccggcgc gttatacagt
     2821 atgctcaagt actacgcccg catgttcccg cacaaggaac tcatcatcat cgaaaatggc
     2881 agcgtcgtca aagccagcgg catcgatcgc gccacctacc tcgaacgcca cgtgagccag
     2941 gtagagcgcg cccgccgccg gggcatcaaa attcccatct acatctgctg gagcatcacc
     3001 tccaaccgtg aatggggtct acctttcggc gacggcaacg acttcggcct ctaccacatc
     3061 gacctggaca ccgaccccac cctggaacgc aaatgcaccg actccgccgg catctacaaa
     3121 cgcctcatcg cgcaacacag cggaactgtg gcaataccat cccccacgga aacacgtaat
     3181 ggcgggactg tggaaacacc cggccaacat ccgtagggac agggggtggt cggaacggtt
     3241 tccagccgaa caggtgcatc acctatgcag cagcaccgga atcaaaagct gcgccgcgta
     3301 gatcaaatgc cacgccgtca ggccgatcgt caccaggacg agcagcatgt gaaatctgcg
     3361 ccagtagcgg atcatgtagc ggtagaactg ctcacgctgc atggcgtgtt ccatctcgcg
     3421 tagatcggag atgtgttctt gcgcgcccgg catcagcgct cctgggcgtg ctaattcgct
     3481 cttccggtca gggacgaagc ggtactgccc cgtctcgcgg ctcaactcct gcggcgtcgc
     3541 ttccagcgaa atgtaggcca ggtcccaggg cgtgtgcagc ggttggttct ggtattcaaa
     3601 ggggaagtgg ttcgagagcg ggccgccgga gagcgagacc agcgaggtcg agcggg
//
LOCUS       DASZAK010000555         1721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_189948, whole genome shotgun sequence.
ACCESSION   DASZAK010000555 DASZAK010000000
VERSION     DASZAK010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189948"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VGT44_09735"
     CDS             complement(<1..251)
                     /locus_tag="VGT44_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581122.1"
                     /translation="MSAIISHLLRASLFLRRFPIPDMRSCMLLLGIAPAGPGCGDSTG
                     ITLMKPVRPGGIWQMVYRRDGPYEAIRWGGISAVLVSTWP"
     gene            238..1632
                     /locus_tag="VGT44_09740"
     CDS             238..1632
                     /locus_tag="VGT44_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581123.1"
                     /translation="MIALIAALPCLNLAVFSWLAAGTMVSRDQWYFIPMVRDYLTGNF
                     HVFSLWETHSQHRTPGYKLLFLLDAGFFRLNMRTEVMLGLAALTASVLLLIRRFHDTL
                     PMDSKPTNLWLGLASFAIVGFNLNQWYDTTYALTAFGGYAGILCFVTLWLMLDTRLRH
                     VERGQGITSLCLMLAFTLMVFAGGIGPALIASMLGVVAIVMLLERKPSKATVILLAWV
                     ALCASACELVYWGTGGIHIATPHSQPFSQVLIQNPSSVLQYLLLSFASSIIPADAMEK
                     HFQGLCHTLNLTVGAGVICLYLGCTWIYLRLRMWKASYLPAFLIAFSTLFILSTLAVR
                     LPATGLEAATAPRYVLYSQLGNLGCLWILFHWFASVGRPRPPSLLSPVSCFAAAFLLY
                     ALGVGALWNYYPSAARNVEEGTQEILTGNFDRPDWLCPDIQLCRTGRATLAQYQLQPF
                     ADRPLAAARGAKQP"
BASE COUNT          315 a          542 c          466 g          398 t
ORIGIN      
        1 ggccatgtcg aaaccaggac cgcagaaatc cctccccacc tgattgcctc ataggggccg
       61 tcacgccggt acaccatttg ccaaatccca cccggtcgca caggcttcat gagggtgatt
      121 cccgtagaat ctccacatcc aggcccggca ggcgcaatgc ctagcagcag catgcacgac
      181 ctcatatccg gtatgggaaa tcgcctgagg aaaagcgatg cgcgcagcag atggctgatg
      241 atcgcactca tcgcggccct tccttgcctc aatcttgcag tcttcagctg gctcgcggcg
      301 ggcactatgg tcagccgcga tcaatggtat ttcatcccca tggtccggga ctatttgacc
      361 ggtaacttcc acgtattctc gctctgggag acccactctc agcatcgcac tccgggttac
      421 aaactgctgt tcctgctgga cgcgggtttc ttcaggttga atatgcggac cgaggtgatg
      481 ctgggcctgg cggcgttgac ggccagcgtg ctgctgctca tacggcgctt ccacgacacg
      541 ctgccgatgg actccaagcc tacaaacctt tggctggggc tcgcgagctt cgccatcgtc
      601 ggcttcaatc tgaatcaatg gtatgacacc acttatgccc tgaccgcgtt tggaggttat
      661 gccggcatcc tgtgcttcgt gaccctttgg ctcatgcttg acacccggct tcgacacgta
      721 gagcgtggcc aggggatcac aagcctgtgc ctgatgttgg cattcactct catggttttc
      781 gcaggtggga tagggcccgc actgatcgcg agtatgctgg gcgtggtggc gatagtcatg
      841 ctattggagc gcaagcctag caaagccact gttattctcc tcgcttgggt ggcactgtgc
      901 gcatcggctt gtgaactcgt ctattgggga acgggtggta tccatatcgc caccccacat
      961 tcgcaaccct tctctcaggt attgatccaa aatccgtcct cggtactgca atacctattg
     1021 ctatctttcg ccagcagcat catccccgct gacgccatgg agaaacactt ccaaggattg
     1081 tgccataccc tcaatctgac ggtgggtgcc ggggtgatct gtctttacct gggttgcacc
     1141 tggatctatc tgcggctgcg tatgtggaaa gcaagctatc tcccggcttt cctgatcgcg
     1201 ttctcgacgc tcttcatcct gtcgaccctc gccgtgcgcc tgcccgcgac cgggctagaa
     1261 gcagccacgg caccccgtta cgtgctctat tcgcagctcg gcaacctggg ttgcctatgg
     1321 atattgttcc actggttcgc aagtgtcgga cgccctcgcc cgccatctct cctcagcccc
     1381 gtatcctgct tcgctgcggc tttcctgctc tatgctctgg gcgtaggggc attgtggaat
     1441 tactacccat cggcggcacg caacgtcgag gaaggcacac aagaaatctt gacggggaat
     1501 ttcgatcgcc cggactggct ttgtcccgac atccagctct gccgcacggg cagagccaca
     1561 ttggcccagt accaattaca gccttttgct gacaggcctc tcgctgcggc gcgcggagcg
     1621 aaacaaccgt gaccgcggtc agggtattgg cccaacacag ggtggatgcc gagatcggcc
     1681 tccttgccac cgcttggtcc gaatccccca cgttcgcgct c
//
LOCUS       DASZAK010000556         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_653854, whole genome shotgun sequence.
ACCESSION   DASZAK010000556 DASZAK010000000
VERSION     DASZAK010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653854"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1520
                     /locus_tag="VGT44_09745"
     CDS             33..1520
                     /locus_tag="VGT44_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEV2581124.1"
                     /translation="MDTIVNTRLGAVRGSIIDGIATFKGIPYAANPFGTDRFRPPQPA
                     QAWDGVRDALAYGPTSPKPGYAAPFDALLPEPAIAGLDCLNLNVWSPDVGRVGLPVMV
                     WIHGGSFANGSGAVPTYNGRRFARDGAVCVTINYRLGPHGFLYLGEGTANLGLLDQVA
                     ALKWVQENIAAFGGDPSNVTIFGESAGAFSVSTLLAMPRAAGLFRRAILQSGAGHHAL
                     QTETSRLIGQYLAEKLGVEPTPDGLASVPFERIIAAQVKLANEVNTRPDPARWREVLF
                     NYMLFEPVIDGEILPSLPMTAIAGGASSDVDVMVGTNTEEWRFFTVPNGVIDMITGDI
                     LAFAVTAYGLSPAETLAFYREALPGASAGDLQAALVTDWFFRVPAIRLAEARTAGKGA
                     TYMYEMAWRSPQFGGRMGACHGLELSFVFDNLDQEENRAMVGDNPPQQLADAMHSAWV
                     AFAISGDPGWSRYDLNRRATMRFDLQSQVVDDPHPAQRMLWEGKR"
     gene            1542..>2073
                     /locus_tag="VGT44_09750"
     CDS             1542..>2073
                     /locus_tag="VGT44_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581125.1"
                     /translation="MIPRDDFTPHGYLDNPHHSWKINPSGVLRSRVPLGMGWHVPNLG
                     SYVRNQFQYTAHLSIGLKIGKTILITPDDFKRHGTTIISTLHTKSRFEYTCTVPKHHL
                     TLTARYFPIDEDALGCVLTVSTTAEYPFPVTCYIMHEHTHNPHTSRLWEHGLYAINGP
                     EEGVGMLGIASEGDVFV"
BASE COUNT          408 a          615 c          591 g          459 t
ORIGIN      
        1 cgtattcgca tacgtatgtt aaggaggtaa acatggacac tatcgtcaat acccggctgg
       61 gtgcggtgcg cggcagcatt atcgatggaa ttgcgacatt caaaggcatt ccgtacgcgg
      121 caaatccgtt tggcaccgat cgttttcggc ctccacagcc cgcacaggcg tgggatggtg
      181 tgcgggatgc gctcgcctat ggcccgactt ctcccaagcc tggctatgcc gcgccctttg
      241 atgcgctgct cccggagcct gccatcgctg gcctggattg tctgaacctc aatgtctggt
      301 cgccggatgt cggtagagtc gggctgccgg ttatggtctg gattcatggc ggctcattcg
      361 caaacggatc gggagcagtg cctacgtaca atggccgccg ctttgctcgc gatggcgcgg
      421 tatgcgtcac catcaattac cgcctcggac cacatggctt tctctacctg ggcgagggaa
      481 cagccaacct cggcttgctc gaccaggtcg ccgccctcaa atgggtgcag gaaaacattg
      541 ctgccttcgg tggcgatccg tccaacgtca ctattttcgg tgaatcggct ggagctttca
      601 gtgtctcaac gctgcttgcc atgccgcgcg cggctggcct gtttcgccgc gctatcttgc
      661 agagcggggc gggacaccac gcgctccaga ccgaaacctc tcggctcatc ggacagtatc
      721 ttgccgagaa gctgggtgtt gagccaactc ctgatggcct cgcatcggtt ccatttgagc
      781 gcatcatcgc tgcccaggtg aagctggcca acgaggtgaa tacacggcct gacccggcgc
      841 gctggcgaga ggtcttattc aactatatgc tgttcgagcc ggtcatcgat ggtgaaatcc
      901 tgccatcgct tcccatgaca gctatcgccg ggggcgcgag ttcggatgtg gatgtgatgg
      961 ttgggacaaa tactgaggag tggcgtttct tcaccgtccc gaatggcgtg atcgatatga
     1021 ttaccggcga tatcctggca tttgccgtca ccgcctacgg actttcgccc gccgagacgc
     1081 tggccttcta ccgggaagcg ctccccggcg cgagcgcggg cgatttgcag gccgctctcg
     1141 tcaccgactg gttcttccgc gtacccgcca ttcgcctggc tgaggctcgc acagcgggca
     1201 aaggggcgac ctacatgtac gagatggcct ggcgttcgcc gcagtttggc ggcagaatgg
     1261 gcgcctgtca tggactcgaa ctgtctttcg tctttgataa cctcgaccag gaagaaaacc
     1321 gggcgatggt gggcgacaac cctccgcagc aactggccga tgccatgcac agcgcctggg
     1381 ttgcctttgc cataagcggc gatccgggct ggtcgcgcta tgatctcaac cgccgcgcga
     1441 ccatgcgctt cgatctgcaa tcgcaggtgg tcgatgatcc gcatcccgca cagcgcatgt
     1501 tatgggaagg caaacgctaa cgaaggaaag aggtatgggt aatgatcccc cgcgacgatt
     1561 tcaccccgca cggctacctg gacaatcccc atcatagctg gaagatcaac cccagtggag
     1621 tgctgcgttc acgtgtcccc ctgggcatgg gctggcatgt acccaatctg ggaagctacg
     1681 tccgcaacca gttccaatac accgcgcacc tatctattgg cctcaagata ggcaagacga
     1741 tcttgatcac tcccgatgac ttcaaacgtc acggaaccac gattatcagc actttgcata
     1801 cgaaaagtcg ctttgaatat acctgcacgg tccccaaaca tcatttgacg cttaccgcgc
     1861 gttattttcc cattgatgaa gatgccctgg gctgtgtcct cactgtttcg acgaccgctg
     1921 agtacccctt tcctgtgacc tgttacatta tgcatgaaca cacacacaat ccgcatacga
     1981 gtcggctctg ggaacatggc ctgtatgcca taaacggacc ggaagaaggg gtaggtatgc
     2041 ttggcattgc tagcgagggt gatgtcttcg ttc
//
LOCUS       DASZAK010000557         4514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_48256, whole genome shotgun sequence.
ACCESSION   DASZAK010000557 DASZAK010000000
VERSION     DASZAK010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4514
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48256"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VGT44_09755"
     CDS             <1..768
                     /locus_tag="VGT44_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581126.1"
                     /translation="IISILAVQFGYNRRQGISRVMAQVGMAMVALGTVVMMVMYVGSA
                     LANWSLPTMFVSGPAGANGIASDDVVTGTLVMGGGLLVAFSLVLVRSCWRRPVRLAAA
                     WAWLLSFATVVVAGFAIEMNEAYFGAGDPAAQGAAKDAVFTWLHQDIGLFLFPTIALV
                     MLAVERLINPYHPARHRWIGWTAIIGTTITFAGGLIFVFVDPSLHGVGYIISTAGLLI
                     VGAMLLATIWWSAIGGYKPGQPATMPPVSKEAHMTSE"
     gene            976..1260
                     /locus_tag="VGT44_09760"
     CDS             976..1260
                     /locus_tag="VGT44_09760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581127.1"
                     /translation="MITRQRKLIRLPIVGIILLGVFAGVYLFAGKRSSKPAAPSRIVK
                     HTVETPPDEALAYWTAEKMRNAKPAPMPNVKAPDRKKRPPRRPSSRPPQA"
     gene            1278..2261
                     /locus_tag="VGT44_09765"
     CDS             1278..2261
                     /locus_tag="VGT44_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581128.1"
                     /translation="MPQLHRFILHPLTSKVFVGLLAGAYIVYLGAFVHNLTASSQPAS
                     QMVIVEHTVDTNLNAAVSYWTVTNMLNATDVDQQLSQTSDVTQMSSDTSAGKAAQQTG
                     QPPLDSSVSYPLSTVGKLFFSSSSGQNLVCSGTAVTSNNKSVVDTAGHCLYWQGGWVQ
                     NVIFCPLFDHGTSPYGCWAARDLAVPAEWINGGSTAFARDFGMAVVAPNDQGALTDVV
                     GGAGWAYNQPIKQLYYPYGYPAAPPFDGQSRQFCEGALGTSWLFGGGTVISVPCNMTG
                     GSSGGPWFIKIGGVLYLSGHNDFTSTIKQGHMFSPYYDDTWYALYSKAQQS"
     gene            complement(2387..2599)
                     /locus_tag="VGT44_09770"
     CDS             complement(2387..2599)
                     /locus_tag="VGT44_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581129.1"
                     /translation="MGLWPFKHETCPIDEEDAILLVDVETESIPLTDEDGNPLYYCME
                     GEHTFALESESVSSSDEGRRGWFWNR"
     gene            3102..3275
                     /locus_tag="VGT44_09775"
     CDS             3102..3275
                     /locus_tag="VGT44_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581130.1"
                     /translation="MVKRTIRWLLPLIALLMFAAYLVVSPIMATHAAPVGAPAAPSIS
                     APHGMTPDYFHRP"
     gene            complement(3319..>4514)
                     /locus_tag="VGT44_09780"
     CDS             complement(3319..>4514)
                     /locus_tag="VGT44_09780"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2581131.1"
                     /translation="YDLLDRESSTILVALEAAHTLDKGAALMRTIYAYIPYQLARSMY
                     NQAEMHLRRAYAAADRKGDTYGKAGALLYLGEIAQKQSNYELATSNLQQGLILARQVG
                     EKERITALLADLGWVTWKRGKYAEAEHYLQEGLALARKINDKEHISDILETLGSVAGS
                     RGEYSKSTKYVEEALEMARGIEDNEKICTLLINLGVTEGERGKYIEAEQYFQEGLQIA
                     HQLGHREWMSLLLSNLGEAESELGNYVQADTYFQDGLRVAREIDHREWISILLNSLGQ
                     TSRKQGYHEQAAAYLQESLLIARQINRPQILSETLDAYGNLCLDRGQKKSADEIYKEM
                     LDVVPEGSQDLIAMAQYGLARAIAAQGRIEEAQYFGGASLTILESMGHRAAKDVRQWL
                     YFITD"
BASE COUNT          961 a         1261 c         1169 g         1123 t
ORIGIN      
        1 atcatctcca tcctggcggt ccagttcggt tacaaccgcc gtcaaggaat tagtcgtgtc
       61 atggcccagg taggaatggc catggtggcg ctcggcacgg tggtgatgat ggtgatgtac
      121 gtggggagcg cgcttgcgaa ctggtcgctg ccgaccatgt ttgtctctgg accggcaggc
      181 gcgaacggca ttgccagcga cgatgtcgtg accggcaccc tggtcatggg tggcgggctt
      241 ctcgtggcgt tttcgctcgt gctggtccgc tcctgttggc gccggcccgt tcggctagcg
      301 gcggcgtggg cctggctctt gagtttcgcc actgtcgtcg tggcgggctt tgcgattgag
      361 atgaacgaag cgtacttcgg cgcaggagat cctgcagcgc aaggcgcggc gaaagacgcc
      421 gtgtttacct ggctgcacca ggatatcggc ctgttcctgt ttcccaccat cgccctggtg
      481 atgctggccg tcgagcggct catcaatcct taccacccgg cgcgccatcg gtggattggc
      541 tggacggcga taatcgggac caccatcacc ttcgcaggcg gcctgatatt cgtgttcgtc
      601 gatccatcgc tccacggtgt ggggtacatc atctccacag cagggttatt aatcgttgga
      661 gcgatgctgc tggcgaccat ctggtggtca gcgattggtg gatataagcc tggccagcct
      721 gcaacaatgc caccggtcag caaagaggcg catatgacca gcgagtaagt cgctcgcact
      781 ccgtcgcctg ccagaggaca ggcgctaaca tctacctctc tggtaagagc ataagggtac
      841 cagagaggta gatgtttcgg tatagggtca aaccctcgtg cctgttcaag cgtatgatat
      901 aattgtaggg atacagcagg caggaatagc gtaacctact cctacgaggt tcccacttat
      961 tgaaagggaa gagctatgat aacacgacaa cgaaaactaa ttcgacttcc catcgttggc
     1021 atcatcctgc ttggtgtgtt tgctggcgtg tacctctttg cagggaagcg ttcttccaaa
     1081 ccggctgctc caagcaggat cgtcaagcat acggtagaga ctccccccga tgaggctctc
     1141 gcgtactgga cggcggagaa gatgcgtaac gccaaacctg ctcccatgcc caacgtcaaa
     1201 gcgcctgatc gaaagaaacg ccctccgcga cggccttcat ctcgtccgcc ccaggcttga
     1261 tgaaagagag tagcataatg ccgcaactgc atcgattcat tctccatccg ctgaccagca
     1321 aggtctttgt tggcttgctg gccggcgcgt atatcgtgta tctgggggca tttgtgcata
     1381 atctgacggc atcgtcgcaa ccagcgtctc aaatggtgat cgttgaacac acggtagaca
     1441 ccaatctcaa cgccgccgtt tcctattgga cggtgacaaa tatgctgaat gccaccgatg
     1501 tcgatcagca gttgagccag acttccgacg taacgcagat gagcagcgac acaagcgcgg
     1561 gcaaggcggc acagcaaacg gggcagcccc cgctcgacag cagcgtttcg tatcccctct
     1621 caaccgtcgg gaaactcttt tttagcagca gcagtggtca aaacctggtc tgctccggga
     1681 ccgccgtgac gagcaacaac aaaagcgtgg ttgatacggc tggccattgt ctttactggc
     1741 agggcggctg ggtgcagaac gtgatcttct gcccgctttt cgatcatggc acgtcgccgt
     1801 atggctgctg ggctgctcgc gatttagcgg ttcctgccga atggatcaac ggcgggtcaa
     1861 ctgcctttgc tcgtgacttc ggtatggccg tcgtcgcccc aaacgaccag ggcgctttga
     1921 ccgacgtggt tggcggggca ggctgggcgt ataatcaacc aatcaaacaa ctttactacc
     1981 cttatggcta tcctgctgcc ccacctttcg atggccagtc aagacagttc tgcgaaggcg
     2041 ctctcggcac ctcctggctg tttggaggcg gcacggtgat ctccgttccc tgcaatatga
     2101 ccggcggctc cagcggcggt ccctggttca ttaagatcgg cggtgtcctc tacctcagcg
     2161 gccacaacga cttcaccagc acgatcaaac agggacatat gttctcacca tactacgacg
     2221 atacctggta cgcgctgtat agcaaggctc aacagtcgta gtgtaacgga gagatgtagg
     2281 ggagcaattc attgcgccct cgtacccggt caagcgctac catcagggcg caatgaattg
     2341 ctcccctaca tctctccgtt gaaaaataga acctctatta ggggtactac ctgttccaaa
     2401 accagccgcg ccttccctcg tcgctgctag agactgactc gctttcgagc gcgaaggtat
     2461 gctcgccttc catgcagtag tatagtgggt tgccatcctc gtccgtcaag ggaatgctct
     2521 ccgtctccac atccaccagc aaaatggcat cctcttcgtc aatcgggcaa gtctcgtgct
     2581 taaacggcca tagacccata gctgtttctc cctctttctg gaacctgcca accacaaagg
     2641 tacccaggtc ccatacgatt tgtttgctct ctcgttaaga catcaacgtc ttctgctggt
     2701 gaaatatact cacttttcgc ggttcttcaa cattccataa atgagacgca agaaagtact
     2761 aacgcataaa aaatttgcgg tggctctgga aaaggttggg tgaggacata gtaccatctg
     2821 gcaggtataa gacagaaacc tgaaaaaacc caaaacttct tataccatct tttcctggaa
     2881 cgatacgtat cgatacggtg tgtgtgatct gcaacttttg cgtctggggg aaaagagggc
     2941 gaccacaaga gtacgctctt accatgacgc gagcaagaca agtgtgtatc tacgccggag
     3001 tcatggtaag ggcgtaccct tgtggtcgcc ctcttttccc agccctggct gaacacatgg
     3061 ttttcgtagt aacccgaaag gaaaagaaag agaggattcc tatggtaaag cgcacgattc
     3121 gttggctcct gccactgatc gctctgctca tgttcgcggc ctacctggtc gtctcgccca
     3181 tcatggccac ccacgctgcg cctgtgggcg ctcccgctgc gccgagcatc tcggctccgc
     3241 atgggatgac gccagattac ttccaccgtc cgtgagatca gactggagga gtaagataat
     3301 actcctcctt tctttcgatt aatctgtgat gaagtataac cactgtcgta catcttttgc
     3361 agctctatga cccatcgatt ccaagatagt taggctcgct cctccaaagt attgtgcttc
     3421 ttcaattctt ccctgagccg caatagcacg agccaatccg tactgagcca tagcaatcaa
     3481 atcctggctt ccttctggta ccacatctag catttccttg tatatctcgt ctgcgctttt
     3541 tttctgtcct ctatcaagac atagattgcc atatgcatct aaagtctcag agagaatttg
     3601 gggcctgttg atttgtctag ctattaataa gctttcttgc aagtatgccg ctgcctgttc
     3661 atgataccct tgtttacgag atgtctgtcc taaactattt aacaatatac tgatccattc
     3721 gcggtggtcg atttctctcg ctactctcag gccatcctga aaataagtgt cagcttgaac
     3781 atagttaccc aattcgcttt cagcttcccc aagattacta agtaataggc tcatccattc
     3841 tcgatgtcca agctggtgtg cgatttgtaa tccttcttgg aaatactgtt ctgcttctat
     3901 atatttccct cgttctcctt ctgtcacgcc taaattaatc aatagagtgc atatcttctc
     3961 gttatcctca atcccgcgtg ccatttcaag tgcctcttcc acatacttcg tcgacttgct
     4021 gtattccccg cgactaccag cgacagagcc gagcgtttcc aaaatgtcgc taatatgctc
     4081 tttgtcattg atcttcctgg cgagagcgag accttcttgc aagtaatgtt cagcctcagc
     4141 atatttgccc cgcttccatg tgacccagcc aagatcggcc agcagggcgg tgatgcgttc
     4201 cttttcgccg acttggcggg caaggatcag cccttgctgc aggttggagg ttgccagttc
     4261 atagttactc tgcttctgtg ctatctcacc tagataaagc agcgcgcccg ccttgccgta
     4321 ggtgtcgcct ttgcgatctg ctgcggcata agcgcggcgc agatgcattt cggcctggtt
     4381 gtacatactg cgggcgagct ggtaggggat gtaggcgtag atggtgcgca tcagagccgc
     4441 gcctttgtcc agggtgtggg ctgcttcgag cgcgaccagg atggtgctgc tctcccggtc
     4501 gagcaggtcg tagt
//
LOCUS       DASZAK010000558         3626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_14214, whole genome shotgun sequence.
ACCESSION   DASZAK010000558 DASZAK010000000
VERSION     DASZAK010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14214"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1554)
                     /gene="hisS"
                     /locus_tag="VGT44_09785"
     CDS             complement(256..1554)
                     /gene="hisS"
                     /locus_tag="VGT44_09785"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV2581132.1"
                     /translation="MPEQFRALQGFKDILPDEQRYWKIIKATASEIAELYGYRLIETP
                     TVEDTSLFRRTSGEGTDIVEKEMYSFDDRPDKEGRSTNISLRPEGTAGVVRAYLEHGM
                     FQQAQPIKLYYLGEPMFRHDKPQAGRVREHHQFGCEALGESDPAIDAEIIGLLFQVYT
                     TLGLRGLNVSLNSIGDQNCRPQYIERLKDYYRPLLETSCDDCKVRFYKNPLRLLDCKE
                     PQDQPKIAAAPKIADYLCDPCREHFAAVRRYLDFYAVPYQRDPLLVRGLDYYTRTVFE
                     VISTASGQALNGGGRYDGLAEILGGKPTPGIGFGAGIERISLEMKQQKIEPPVEAALR
                     AFIVSFGKTPEYKDAVVQLIADLRHAGIRAEMSYGERSAKSQLKRADASGAAYAILIG
                     ENELAGGFVTVKDLQAAGMGQEGKQIEVKRDGLVAFLRSE"
     gene            1897..3000
                     /locus_tag="VGT44_09790"
     CDS             1897..3000
                     /locus_tag="VGT44_09790"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2581133.1"
                     /translation="MKRYGGVAAGGNDSQHSTVLSVIIPLKNEEENIPLLHAALSRVL
                     QEQGYGYEILYVDDGSTDHSYAQLQHVAAFDAHARVIRLSRNFGQTAAISAGVEQSCG
                     DILIFMDGDLQNDPIDIPRLLAKLDEGYDVVSGWRKHRQDAKLSRKLPSWLANRLISK
                     VSGTYLHDYGCTLKAYRREVFEHVRLYGEMHRFIPAYAAIAGASIAEIEVTHHPRRFG
                     VSKYGISRTFRVMLDLLTLKFLGSFGTKPLYPFGVPGIASLGMGSVLATSVLLRRAFP
                     PHVRANRNPLLLLAVFFSGFGMQSIMMGLLAEMLMRTYHESQGKATYVIRDILPGIIA
                     DEQYSGAQRLPAEQTTDEIGVTQKQPAMVRGNR"
     gene            3001..>3626
                     /locus_tag="VGT44_09795"
     CDS             3001..>3626
                     /locus_tag="VGT44_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2581134.1"
                     /translation="MPNLPDTPGASVLMIAPQPFFESRGAPFCVYQHVKALTTLGYKV
                     DLITYPIGKSVRLPGLRIFRAPSIPFIHSVKPGPSLAKIPLDIAVFLTALLRLCQRKY
                     AFIHTHEEAGLMGVVLAPLFGYKHLYYMHSDLSQVVASSEFTRSAWLMRCVGAAQRFM
                     IRKADVVIAFYPEIVKAAQALASGKAIYQILPPAVDEDLPPAKESDVER"
BASE COUNT          693 a         1012 c         1084 g          837 t
ORIGIN      
        1 gaacgtgaag ctgcggcaga ctggtattac ctcattaaag atgaggaagg tctggtaatt
       61 gcttcaacga gcatatctaa aggcgctaaa gagaccttgc gtgataaccg ggtaagttaa
      121 atggcaaggc aacttcgttt cgataatggt agacagcttg ttgagttcgt tagctgtacc
      181 atcagtcgcg cagatgcgct taagattata aagcgcaact atttggaacg ccgacgcagc
      241 taaaagcttc cttcgttact cgcttcgcag aaacgcaacc aatccgtccc tcttcacctc
      301 gatctgtttg ccctcctgtc ccatcccggc ggcttgcagg tctttgacgg ttacgaaacc
      361 gcctgccagt tcgttttctc cgatcaggat ggcgtaggcc gcgccggatg cgtcggcgcg
      421 ttttagctgt gacttcgcgc tgcgctcgcc gtagctcatt tcggcgcgaa ttcctgcatg
      481 gcgcaggtcg gcaatgagct gcacgacggc gtctttgtat tcgggtgttt tgccaaagga
      541 gacgatgaag gcgcgcaggg ctgcttccac cggtggttcg atcttctgct gtttcatctc
      601 cagcgagatg cgctcgatgc cagcgccgaa gccgatgccc ggcgttggtt tgccgccaag
      661 gatctcggcc aggccatcgt agcgtcctcc accgttgagg gcttgcccgg aggctgttga
      721 gatcacttcg aagacggtgc gcgtgtagta gtccaggccg cgcaccagca gcgggtcacg
      781 ctggtaggga acggcataga aatcaaggta gcggcgcacg gcggcgaaat gctcgcggca
      841 cgggtcgcac aggtaatcgg cgattttggg cgcggcggcg attttgggct gatcctgcgg
      901 ctctttgcaa tccagcaggc gcaagggatt cttgtagaaa cgcaccttgc agtcgtcgca
      961 gctcgtttcc aggagaggac gatagtagtc tttcagccgc tcgatatact gcgggcggca
     1021 gttctggtcg ccgatgctgt tcagcgagac gttcagcccg cgcagcccca gcgttgtata
     1081 gacctggaag agtagaccga tgatctctgc gtcgatggcc gggtcgctct cgcccaacgc
     1141 ttcgcagccg aactggtggt gttcgcgcac gcggcctgcc tgcggcttgt catggcgaaa
     1201 catcggctcg cccaggtagt agagcttgat gggctgcgct tgctgaaaca tgccatgctc
     1261 caggtaggcg cgcacgacgc cagccgtgcc ttcgggccgc agggagatgt tcgtgctgcg
     1321 cccctcttta tcggggcggt cgtcgaagct gtacatttct ttctcgacga tatcggtgcc
     1381 ttcgccgctg gtgcgccgga agaggctggt gtcctccacc gtcggcgtct cgatcagccg
     1441 gtagccatac aactcggcta tctcgctcgc ggtggccttg ataatcttcc agtagcgctg
     1501 ttcgtcgggc aggatatctt tgaagccttg caaggctctg aattgttcgg gcataggtac
     1561 gtctctcttt ctcctccacg ctgtttgccc cgctattata gcagattgcg ccctatgagg
     1621 gaaagtcgta ggagattcca gggaaccgaa gggcgaccgt tggggtacgc ccttcggttc
     1681 cctgcgttat ttggacatac ccgtacaatg ttattttgag ggggtaaaca actgcttgat
     1741 gccccgcgtc ctgttcagcg cgacgctgat agggattttt cgtctctgat gttggtaggg
     1801 aacggtgtgc ctttcgctac aatgagctgg ttcccggcaa cggggacgcc ttgttgagta
     1861 gtgttggaca tcgtaaggaa ggataggatt gacctcatga aacgctatgg tggtgtggct
     1921 gctggtggga atgatagtca acatagcaca gtactttctg tcatcattcc gttgaagaac
     1981 gaagaggaaa atatacccct gctgcacgcg gcattgagcc gtgttttgca ggagcaggga
     2041 tatgggtatg agatcctcta tgtcgatgat ggcagtaccg atcattcata tgcacagttg
     2101 cagcatgtgg cggcatttga tgcgcacgcg cgcgttatcc gcctcagccg caactttggg
     2161 cagaccgccg cgatctccgc gggtgtggaa cagagctgcg gcgatattct gatctttatg
     2221 gatggtgatc tccagaacga tcccattgat ataccgcgac tgctggcaaa actcgacgag
     2281 ggatacgatg tcgtcagcgg ctggcgcaaa catcgccagg acgcgaagct gagtcgcaag
     2341 ctgccctcgt ggctggccaa tcgcctcatt tctaaggtga gcggcacgta tctgcacgac
     2401 tatggttgta cgctcaaagc ctaccgccgc gaggtgttcg agcatgtgcg gctctacggc
     2461 gagatgcatc gctttatccc ggcatacgcg gccatcgcgg gcgcgtctat cgccgagata
     2521 gaagtaacgc atcatccccg gcgttttggc gtatcgaagt atggaatttc gcgcaccttc
     2581 cgcgtcatgc tcgatctgct gaccttgaag ttcctgggca gctttggcac aaagccgctc
     2641 tatccctttg gggtgcccgg tattgcctcg cttggcatgg gcagcgtact ggccacctcg
     2701 gtcttgctgc ggcgcgcgtt cccgccgcat gtacgcgcca accgcaaccc gctgcttttg
     2761 ctggccgtgt ttttctccgg ctttgggatg caatccatca tgatgggcct gctggccgaa
     2821 atgctgatgc gcacgtatca cgaatcacag ggcaaagcaa cctacgtcat tcgcgatatc
     2881 ctgcctggca tcattgctga cgagcagtat agcggcgccc agcggctccc tgccgagcaa
     2941 acgaccgacg agatcggtgt gacgcagaag caaccggcga tggtgagggg gaatcgatag
     3001 atgcccaacc tgccggatac gccgggggcg tcggtgctga tgatcgcccc gcagcccttt
     3061 ttcgagtcgc gcggcgcgcc gttttgtgtg taccagcacg tgaaggcgct gaccacgctg
     3121 ggttacaaag ttgacctgat tacttatcca atcggtaagt cggtcaggct gccgggcttg
     3181 cgcatcttcc gcgcgccctc gatcccattt atacacagcg tcaagccggg gccttcgcta
     3241 gccaagatcc ccctggacat cgccgtcttt ctcactgcgc tgctgcggct ctgccagaga
     3301 aaatatgcgt tcattcacac gcacgaagag gccggcctga tgggggtggt gctggcaccg
     3361 ctcttcggtt acaagcacct gtactatatg cattccgacc tgtcgcaggt agtagccagc
     3421 tctgaattta ccaggagcgc gtggctgatg cgctgtgtcg gggccgcgca gcgcttcatg
     3481 atacgcaaag cggacgtagt gatcgccttc tatccagaaa ttgtgaaggc ggcgcaggcg
     3541 cttgcgtccg gcaaagccat ctaccagatt ctgccgccag ccgttgatga ggacctgccc
     3601 cccgcgaaag agagcgatgt tgaacg
//
LOCUS       DASZAK010000559         2506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_119996, whole genome shotgun sequence.
ACCESSION   DASZAK010000559 DASZAK010000000
VERSION     DASZAK010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119996"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..718)
                     /locus_tag="VGT44_09800"
     CDS             complement(8..718)
                     /locus_tag="VGT44_09800"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HEV2581135.1"
                     /translation="MSVFLTDLLTFFQTYGYPALWVSVFIAAVGAPLPIGLVLLAAGA
                     FAALGDFNVFLLALITVTASVAGDNVGYLVGKKWGSHALDWLDRSRFGSRMLPPRAIE
                     RSRAYFKHRGGWAVFLSRFLVSALGGIINLLAGSELFPYRAFLACDLAGETVGAIMSL
                     ALGFAFGASWEAVGDIVGSLSLFFVAMLVAALLCVRLLRYLSNSKQASQKIEIQSIPG
                     LTPIIDPPAPSSGPLTLS"
     gene            complement(715..1164)
                     /locus_tag="VGT44_09805"
     CDS             complement(715..1164)
                     /locus_tag="VGT44_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581136.1"
                     /translation="MITRINPRLKLFLTGFLLCIALGVAIFCAIQTYQAFQRFQQSRN
                     LTLSGDVSTIRPWMTIPYIARVYHVPASYLDEQLRISNPQDQSRASLHTLATRYNRPL
                     DRVIHDVQQAILNYRKQHPTHTLHPPGLPHPAITPKTTPTISRRMPG"
     gene            1250..1687
                     /gene="dut"
                     /locus_tag="VGT44_09810"
     CDS             1250..1687
                     /gene="dut"
                     /locus_tag="VGT44_09810"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV2581137.1"
                     /translation="MQPLEVTIKRIDKDLPLPTYATQGSVGFDLYCREETEVAPRSIE
                     LIPANVIVRIPAGYMLMLTMRSSTPRRKGLLIPNGIGIIDQDYCGEGDELRVQVFNFR
                     EEAVTVKRGERIAQGIFVPIARAIWIEADEMGQGRGGFGSTGV"
     gene            1701..2036
                     /locus_tag="VGT44_09815"
     CDS             1701..2036
                     /locus_tag="VGT44_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV2581138.1"
                     /translation="MQDFQDYYALLGVKPDATVQEIKRAYHRLARLHHPDLNQTALDD
                     HIKRLNEAYEVLRNPQKRSDFDAQLAQRATKITAQKDRKQARREPEMSWVEGAFGFVK
                     ELKKGLKDE"
     gene            2175..>2506
                     /locus_tag="VGT44_09820"
     CDS             2175..>2506
                     /locus_tag="VGT44_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581139.1"
                     /translation="MAGTWKTFQAPDTPSGVFSADTMILLTDGSVLIHNGNDTNAVTP
                     CKEWLRLTPDAQGNYATGAWSGVIAMTNAREFFSSGVLRDGRVYVIGGEYSDDLADDT
                     FNNPPTGQ"
BASE COUNT          591 a          637 c          791 g          487 t
ORIGIN      
        1 aaaaaagtta cgaaagcgtt agaggaccgg aagatggcgc gggtggatcg atgattggcg
       61 ttaagccggg gatactctgt atctcgattt tctggcttgc ctgctttgag ttgctgagat
      121 agcgcagcag gcgcacgcaa agtagcgcgg caaccagcat ggcaacgaag aagagggaga
      181 gcgagccgac gatatcgcct acggcttccc agctcgcgcc gaaagcgaat cccagggcaa
      241 gggacataat tgcgcctacg gtttctcctg cgagatcgca ggccagaaat gcgcgatagg
      301 ggaagagttc cgagccggcg agcaagttga taatgccgcc gagggcggag accaggaaac
      361 ggctgaggaa gactgcccag ccccctcgat gcttgaagta ggcgcgcgac cgctcgatag
      421 cgcgtggcgg caacatgcgc gaaccaaaac gcgaccgatc cagccagtcc agggcgtggc
      481 tgccccactt tttgccaacc aggtagccga cgttatcccc cgcgacggag gcagtaacgg
      541 tgataagggc cagcaggaag acattgaagt cgccgagcgc ggcgaacgcg ccagccgcga
      601 gcaaaaccag cccgatcggc agcggcgcgc ccaccgcggc gatgaacacg gaaacccaca
      661 gcgcaggata accgtaggtc tgaaaaaacg tcagcaaatc ggtgaggaat acactcatcc
      721 aggcatcctc cggctgattg tgggtgtagt tttaggcgta atggccgggt gaggtaaacc
      781 aggagggtga agtgtgtgtg tcgggtgctg cttgcgatag ttcaggatgg cctgctgtac
      841 atcatggatt actctgtcga gcgggcggtt atagcgcgtg gccagggtat gcagggatgc
      901 gcggctctgg tcttgcgggt tagagatacg cagttgctcg tccaggtagc ttgcgggtac
      961 gtgatatacg cgcgcgatgt agggtattgt catccagggg cgaatggtac tgacatcgcc
     1021 tgagagcgtc aggttgcgac tttgttgaaa acgttgaaat gcctggtagg tctgtatggc
     1081 gcagaaaatg gcgacgccca gcgcgatgca tagcagaaag cctgtcagaa acagtttcag
     1141 acgagggttt attcgcgtaa tcaccgtact gctctccata attgctcggt tcacctggct
     1201 cttgacgttg caacaactct cctctagaat agcagatatg gagatagcag tgcaaccatt
     1261 ggaagtgacg attaaacgca tcgataaaga cctgcctttg cccacctatg ccacgcaggg
     1321 atcggtgggc ttcgatctat attgccgtga agagacggag gttgcgccgc gcagcatcga
     1381 actgattccc gccaacgtga tcgtgcgcat cccagccggg tatatgctga tgctgaccat
     1441 gcgaagcagc acaccacgcc gtaaggggtt gctaattccc aacggcatcg gcattatcga
     1501 ccaggattac tgtggggaag gtgatgaact gcgggtgcag gtgttcaatt tccgcgaaga
     1561 ggcggtcacg gtcaaacgtg gggagcgcat tgcgcagggc attttcgtac ctattgcgcg
     1621 cgcgatatgg atcgaggcgg acgaaatggg acagggacgc ggcggcttcg gcagcacggg
     1681 agtatagggg catagaagat atgcaggact ttcaggatta ttacgctctg ctgggggtga
     1741 agcccgatgc caccgtgcag gagatcaaac gcgcctacca ccgtcttgcg cgcctgcatc
     1801 accccgacct gaatcaaacg gccctggacg atcacatcaa acgcctcaat gaagcctatg
     1861 aagtgctgcg caatcctcag aaacgttccg atttcgatgc gcaacttgct cagcgcgcta
     1921 cgaagattac cgcgcaaaaa gaccgtaagc aggccaggcg cgagccggag atgagctggg
     1981 tggaaggagc gttcggtttc gttaaggagc tgaaaaaggg tttgaaggac gagtaaatga
     2041 aagggcgtga atgttgtgta ggggcgcaat tcattgcgcc cgcttgccag gcggcttcga
     2101 ccggtgacgt gggcgcgatg aattgcgccc ctacacggct tttgcaccgt gatgaaccag
     2161 gagagagaaa ttgtatggcc ggtacatgga agacttttca agccccggac acgccttccg
     2221 gagtgtttag cgctgatacc atgattcttc tgaccgacgg gtcggtcttg atccacaatg
     2281 gcaacgatac caacgccgtg acgccctgca aagaatggtt gcgccttacg cccgatgcgc
     2341 agggcaatta cgctaccggc gcgtggtcgg gcgtgattgc gatgaccaac gcgcgggaat
     2401 tcttctcgtc cggcgtgctg cgagatggca gagtttacgt gattggcggt gagtactctg
     2461 atgacctggc cgatgataca ttcaacaatc cgccaacggg gcaaga
//
LOCUS       DASZAK010000560         4440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_689231, whole genome shotgun sequence.
ACCESSION   DASZAK010000560 DASZAK010000000
VERSION     DASZAK010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689231"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..820
                     /locus_tag="VGT44_09825"
     CDS             146..820
                     /locus_tag="VGT44_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2581140.1"
                     /translation="MDSRRRHWSQQQQLFREALFSKTRHDEAMRLLLSQHAMLHSAQM
                     AGSGEWSFEDEVLAGLSDEQLRTRPLATGNSIAWLIWHVARIEDVTMNLLVAGRPQVF
                     HEMNWPERLEIQRRDVGTSMSDSEVADFTRQVSLAALRAYRMAVGRGTRAIIAALQPA
                     EVWERVDPERVRQLVAEDALAQGALDLARFWGGRRKAGILTMPATRHSYTHLSEARAV
                     RGKLLR"
     gene            complement(824..3301)
                     /locus_tag="VGT44_09830"
     CDS             complement(824..3301)
                     /locus_tag="VGT44_09830"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEV2581141.1"
                     /translation="MASWWKKAAAATAGMAGLAGSAYFVLTRRPLPRKKGQVSLQGLH
                     EPVEIITDRYGVPHIYAQNEDDLFFGQGYVHASERLWQMELNRRIGAGRLSEIFGDLA
                     LEADRFARRLGMHRSAAAAVEQLSAHSHRILAAYTRGVNAFIEGHRRNLPLEFLLLRF
                     QPDPWKMADSIQWSKMMGWNLGGNWETEIIRARIVAKLGAERAAKLEAGYDPKHPLIV
                     PPGVEYQGINLGMLEQYEQLKQLSGFGLLGGSNNWVVDGTMTTTGAPILCNDPHLGQA
                     APSIWFECHLVAGDIDVEGVSFPGTPGIVIGHNQYVAWGLTNAVSDVEDLYIEKFNPR
                     NPRQYEYMGKWEEAQVIREEISVKGSAPVIEEVRITRHGPIIASYTPPTAPAAEADKS
                     GSNGHLSPAGEARELPLALRWAGLEKHDILSAVSKMNRATSWQELREGLRDWDSPPQN
                     VVFADTQGNIGYVMAGAIPIRKQGQSLLPSPGWTGEYEWTGYIPFDELPQTINPEQHF
                     IATANNRVVDDAYPYYITNEWLNGYRAQRIRDLLTSKGRLSLSDMASIQADQYALPAV
                     EIVPHLLKLRPTTPTGQAALDVMRAWNKVLSSDSAGAAIYSAFLWRLERMVFAAVLGD
                     EETLLHGFLGVGSTILAPQNGYASRSKPLLIRLLNERDDGWFAGSVIANGPRTWDAAL
                     NSAFEAAIEDLGDKLGDNVARWRYGAIHKMTYNHPLGMIKPLDRIFNRGPFPVGGDID
                     TVNMGASLVNQPETVITVPSYRQIVNLADLKASLSIHHPGQSGHPASKRYDDFIKPWL
                     NVEHHPMLFDRAMIEENAAGTLHLTPR"
     gene            complement(3387..4007)
                     /locus_tag="VGT44_09835"
     CDS             complement(3387..4007)
                     /locus_tag="VGT44_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581142.1"
                     /translation="MRMTSIWYEMLPVTLEAEQVVALYRREDDEAQAWLVPHQAGMHP
                     NDAIIAHLERFFGDAFEPGASIVHSTSWRYCHETERLILTYLVVIPQRAWSRATSGKF
                     VARRLGGRIEQARGDNLHPPENIELNNILAHALDHLAMLNSYDERINEALESDWMGVL
                     ETRIPKPAGYVARLFQSPQSEGLDDMATARDRHYDTAGGRRFYRVS"
     gene            complement(4008..>4440)
                     /locus_tag="VGT44_09840"
     CDS             complement(4008..>4440)
                     /locus_tag="VGT44_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581143.1"
                     /translation="VAAAQNGLNNLRGDLAALPVEDAPANGWAEQSARALKAFHDAIN
                     DDLDLPVALSIAREMARDGKVAPGERRRLLLDFDRVLGLRLDTVQPAQPKAISEEALA
                     LVRERDAARVARDWKRSDQLRDRLVALGYEVRDTPKGTELV"
BASE COUNT          847 a         1374 c         1348 g          871 t
ORIGIN      
        1 atcctgcgcg ccttccgcga actgttcgtc ttcgctatga agtggcatcg cgaggagagg
       61 caggcggaac gcgagcgcga aaagctggag aagtcgcaga aacagcattc cgcacaaaaa
      121 agacccgcga gtgtgcagaa ataagatgga cagccgccgg cgacactggt cgcaacagca
      181 acaactcttt cgcgaggcgc ttttctcaaa aacgcgccat gacgaggcca tgcgcctgct
      241 gcttagccag catgccatgc tccactccgc gcagatggcc ggaagcggcg agtggtcgtt
      301 tgaagatgaa gtactggcag gattaagtga cgagcagctc cgcacacgtc ccctcgcaac
      361 cggtaactcg attgcctggc tgatctggca cgttgcccgc attgaagatg tcaccatgaa
      421 tctgctcgtg gcaggccgcc cacaggtgtt tcatgaaatg aactggccgg agcggctcga
      481 gattcagcgc cgcgatgtcg gaacctcgat gagcgacagc gaggtagccg attttacccg
      541 ccaggtgagc ctggccgcgt tgcgcgccta tcgcatggcg gtcgggcgcg ggacgcgagc
      601 catcatcgct gcgctccaac ctgccgaggt ctgggagcgt gtcgacccgg agcgcgtccg
      661 ccagctcgtg gccgaggatg cgcttgcgca gggcgcgctt gacctcgcac ggttctgggg
      721 cggtcggcgc aaagccggta tcctcaccat gcccgccacg cgccacagct acacccatct
      781 gagcgaggcg agagccgtgc gagggaaact gctcagatga tcgctaccgg ggcgtcaaat
      841 gcagcgttcc cgccgcattc tcctcgatca tggcgcggtc gaacagcatg ggatgatgct
      901 ccacatttag ccagggtttg atgaaatcgt cgtagcgctt gctggcggga tggccggatt
      961 gcccaggatg atgaatcgac agcgacgcct tgagatcggc caggttgacg atctggcgat
     1021 aggacggcac ggtaatcacc gtttccggct ggttgaccag cgacgcgccc atgttgaccg
     1081 tatcgatatc gccgccgacg gggaagggac cgcgattgaa gatgcggtcg agcggcttga
     1141 tcatccccag gggatggttg taggtcatct tgtggatcgc cccgtagcgc cagcgcgcca
     1201 cgttgtcgcc cagcttgtca cccaggtctt cgatagcagc ctcgaatgca ctgttcagcg
     1261 ctgcatccca tgtcctgggg ccgttggcga tgaccgagcc ggcgaaccag ccatcgtcac
     1321 gctcgttgag caggcgaatg agcagcggct tgctgcggct ggcgtagccg ttttgcgggg
     1381 ccaggatggt cgatccaact cccaggaagc catgtagcaa tgtctcctcg tcgcccagca
     1441 cagccgcgaa aaccatgcgc tccaacctcc acaggaacgc gctatagata gccgcgcccg
     1501 cgctgtcgga agacaagacc ttgttccagg cacgcataac atccagggca gcctgcccgg
     1561 tgggcgtagt tggtcgtagt ttgagcagat gtggcacgat ttcgacggcg ggcagcgcgt
     1621 actggtcagc ctggatgctg gccatatccg acagcgagag cctgcccttg ctggtcagca
     1681 ggtcgcgaat gcgctgcgcg cggtagccat tcagccactc gttggtgatg tagtaaggat
     1741 aggcgtcatc cacgacgcga ttattggcgg tggcgatgaa atgctgctca ggattgatcg
     1801 tctgcggtag ttcatcgaag gggatataac ccgtccattc atactcgccc gtccagcccg
     1861 gcgaaggcaa cagcgactgc ccttgcttgc ggatagggat cgcgccggcc atgacgtaac
     1921 cgatgttgcc ctgcgtatcg gcgaagacga cgttctgcgg tggcgagtcc caatcgcgca
     1981 ggccctcgcg caattcctgc cagctcgtgg cgcggttcat cttcgagacg gcggagagaa
     2041 tatcgtgttt ttccaggcca gcccagcgca gcgccagcgg taattcgcgc gcctcgcccg
     2101 cgggggacag gtggccgttg cttcccgatt tatccgcttc cgctgctgga gctgttggcg
     2161 gcgtatagct ggcgatgatg ggaccgtgcc gggtgatgcg cacctcttcg ataaccggcg
     2221 cggaaccctt gacgctgatc tcctcgcgaa tcacctgcgc ctcttcccac ttgcccatgt
     2281 attcgtactg gcgtggattg cgcgggttga atttctcgat gtacaggtct tcaacatcgg
     2341 agacagcgtt ggtcaggccc caggcgacgt actggttgtg gccgatcacg atgccaggcg
     2401 tgccgggaaa actgacgccc tcgacatcga tgtcccccgc gacaaggtgg cactcaaacc
     2461 agatagatgg cgcggcctgt ccaagatggg ggtcgttgca gaggatgggc gcgccggtcg
     2521 tggtcatcgt gccatccacc acccagttgt tgctgccgcc cagcagcccg aagccgctca
     2581 actgcttcaa ctgctcgtac tgttccagca tccccaggtt gatgccctga tattcaacgc
     2641 ccggcgggac gatcaacgga tgcttcgggt cataccccgc ttccagcttc gcggcgcgtt
     2701 cggctcctag tttggcgacg atgcgcgcgc gaatgatctc agtctcccag ttgccgccca
     2761 ggttccagcc catcatcttc gaccactgga tgctgtcggc catcttccag ggatcgggtt
     2821 ggaaacgcag cagcagaaat tcaagcggga gattgcggcg gtggccttcg ataaaagcat
     2881 tgacgccgcg cgtgtaggcc gctagaatgc ggtggctgtg cgcggacaac tgctcaacgg
     2941 cagcggcggc ggagcggtgc atccccaggc ggcgggcaaa gcggtcggcc tctagcgcaa
     3001 gatcgccgaa gatttcggag agccggccgg ccccgatgcg ccggttcagt tccatctgcc
     3061 acaaccgctc cgacgcatgt acatagccct gcccgaaaaa cagatcatct tcgttctgcg
     3121 cgtagatatg cggtacgccg tagcgatcag tgataatctc aaccggttcg tgcagccctt
     3181 gcagggagac ctgtcccttc ttcctgggca atgggcggcg cgtcaggacg aaatatgcgc
     3241 tacccgccag ccccgccatc ccagcggtcg cagccgcagc ctttttccac catgatgcca
     3301 tgccgtttgc tccttcgtag tcttccaggt acgtatcagt agtatagcat tgaaaaaccc
     3361 aacatggcga ccgcaaggga tcgccactac gatacccgat agaatcgacg accgcctgcg
     3421 gtatcgtagt ggcgatccct tgcggtcgcc atgtcatcca atccctccga ttgcggactt
     3481 tgaaacagcc ttgcaacata tcccgctggc ttcggtatcc tcgtctccag cactcccatc
     3541 caatcggatt cgagtgcctc gttgatacgc tcatcatagc tgttcaacat ggcaagatga
     3601 tcgagcgcgt gtgcaaggat gttgttcagc tcgatgttct caggtggatg taaattatcg
     3661 ccgcgcgcct gctctatcct gccacctaat cgtcgcgcga caaatttgcc gctcgtagcc
     3721 cggctccacg cgcgttgcgg aatcaccacc aggtaggtga gaataaggcg ttcagtctcg
     3781 tggcaatagc gccacgaggt ggagtgcaca atcgatgcgc ccggctcgaa cgcatctccg
     3841 aagaaacgtt caagatgtgc aatgatagca tcgtttggat gcataccggc ctggtgaggc
     3901 acaagccagg cttgcgcctc atcatcctct cggcggtaca gcgccactac ttgctccgct
     3961 tccagtgtca cgggcagcat ttcataccag atcgatgtca tacgcatcta caccagttcc
     4021 gttccctttg gcgtgtcgcg cacttcgtag ccaagtgcga cgaggcggtc gcgcagctgg
     4081 tcggagcgtt tccagtcgcg ggcgacgcga gcggcgtcac gttcgcgcac cagcgccagc
     4141 gcctcctcgc tgatggcttt aggctgcgcg ggctggacgg tatccaggcg cagtcccaga
     4201 acacggtcga agtcgagcag caagcggcgg cgctcgccag gagcgacctt gccgtcgcgc
     4261 gccatttcgc gcgcgatgct gagcgccacc ggcaaatcca ggtcgtcgtt gatggcatca
     4321 tggaacgcct tcaatgcgcg cgcggactgc tccgcccaac cgttcgcagg cgcatcctcg
     4381 acgggcagcg cggccaggtc gccgcgcaga ttgttcagcc cgttctgcgc cgccgcaacg
//
LOCUS       DASZAK010000561         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_190164, whole genome shotgun sequence.
ACCESSION   DASZAK010000561 DASZAK010000000
VERSION     DASZAK010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190164"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..965)
                     /gene="rsmI"
                     /locus_tag="VGT44_09845"
     CDS             complement(261..965)
                     /gene="rsmI"
                     /locus_tag="VGT44_09845"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV2581144.1"
                     /translation="MLYLVATPIGNLEDITLRALTTLREVDYVASEDTRKTGLLLKHF
                     DIARPQISFHEHNEERAGQRIEELLKQGKSVALVTDAGTPGIADPGYTLVRRAVANDI
                     PVTMIPGATAFATALVLSGLPVHSFTFRGFPPRKPGQRRRFLAIDKDAPHTLIYYESP
                     YRLQAFLADAKEVLGEREAALANDLTKLFEHVERGTISTLMAWVEGQAKLRGEFVVVI
                     GGMEKRGNAREEEEEG"
     gene            complement(980..>2498)
                     /locus_tag="VGT44_09850"
     CDS             complement(980..>2498)
                     /locus_tag="VGT44_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2581145.1"
                     /translation="QTSVALLEPDEKGEVVSLTYGELAERVHRFAGYLQEQDAVKGQR
                     FLIWAASSMNWMVAFLAVLHVGGVAVPLDVSSREDFIARIIETTGATHLITTAKQYSS
                     LKKSLVPLIDIDALPAGAYNAVKLPSLGAHDLAELVFTSGTTGVPKGVMLSHGNIVSD
                     IDAALKVVPIQSSDRALSILPLSHMFEMTIELAIFRSEGSILYARSLNPETLLKLLGT
                     QGITCMVLIPQALQLFMNGIDRQVRAQKREKQFEGLLRLARWLPFGLRPLLFRPVHKR
                     FGGHFRFFISGGAYLPPRLAERWELLGIRILQGYGATECAPVVSVTPPSDHNFESVGK
                     PLPGIDVRIAEDGELLVRGPIVALGYWHNPEATAAAFQDGWYHTGDLGYQDSDGSLYL
                     KGRKKNMIVLSNGLNVYPEDIENVLLTNPAIKDAVIVGLMEDDGGPVVHGVLLMDDPG
                     QAKAVVQQINKQLASHQQIRSYTIWPDGDFPRTHTLKVKRPELLEKLQALRGQHS"
BASE COUNT          524 a          732 c          717 g          525 t
ORIGIN      
        1 cggcctcgaa gaagtcatcg agatcgaact gaccagcgaa gagcaggcac tgctacagaa
       61 gtcggccagc gctgtgaagg agttggtcga ggtaatgggg atttgattaa gtaatcaatg
      121 aggtatatct gtaggtgccg atttatcgcg cacaccgccg ataaatcggc ggtgtgcgcg
      181 ataaatcggc acctacagtg gacccgcttt aattttgaaa atttatcatc gagcaaggaa
      241 accacaacta ttgaagtaat ttaaccttcc tcttcctcct ccctcgcatt cccgcgtttc
      301 tccatccctc ctatcaccac cacaaactcc cccctcaact tcgcctgtcc ctctacccac
      361 gccatcagcg ttgaaatcgt cccacgctcg acatgttcaa acagtttcgt caggtcgttg
      421 gcgagcgcgg cttccctctc tcctagcacc tccttagcat ccgcgagaaa ggcttgcagg
      481 cggtacgggc tttcataata gatgagagtg tggggtgcat ccttgtcgat ggccaggaag
      541 cggcggcgct ggccgggctt acgcggaggg aagccgcgaa aggtgaagct gtggacgggc
      601 aggccggaga gtaccagcgc ggtcgcgaag gccgtcgcgc cgggaatcat ggtgacggga
      661 atatcattgg cgacagcgcg gcgcaccagc gtgtagccgg ggtcggcgat gccgggcgtc
      721 ccggcgtccg tcaccagcgc gaccgatttg ccctgtttca acaattcttc gatgcgctgg
      781 cccgcgcgct cctcgttatg ctcgtggaac gagatttgcg ggcgcgcgat gtcgaaatgc
      841 ttgagcagca ggccggtctt gcgcgtatct tcgctggcga cgtaatcaac ttcgcgcagc
      901 gtggtcaaag cgcgcagtgt gatatcctcc aggtttccga tgggcgtggc gactaaatac
      961 agcatcgcat ctcgttccat tatgaatgtt gtccgcgcag cgcctgcaac ttctccagta
     1021 gctcaggccg tttgactttg agggtgtggg tgcgtgggaa atcgccatcc ggccagatgg
     1081 tgtaggagcg tatttgctgg tgcgaggcaa gctgtttgtt aatttgctgg acgaccgcct
     1141 tcgcctggcc ggggtcgtcc atcaatagaa cgccatgcac gaccgggccg ccatcgtctt
     1201 ccatcagacc cacaatcacg gcatctttga tagccggatt ggtcagcagg acattctcga
     1261 tgtcctctgg atagacatta agtccattcg agaggacgat catgtttttc ttgcgtccct
     1321 ttaagtagag acttccatca ctgtcctggt agcccaggtc gccggtatga taccaaccat
     1381 cctggaaggc ggcagcagtg gcttcgggat tgtgccagta gcccagtgcg acaattggcc
     1441 cgcgcaccag cagctcgccg tcttcggcaa ttctcacgtc gatacccggc aggggcttgc
     1501 caacactctc gaagttgtga tcggaaggcg gcgtgacgga gacgacggga gcgcattcag
     1561 tcgcgccata gccctgcaag atgcgtatgc cgagcagctc ccagcgctcg gccagcctgg
     1621 gcggcagata cgcgccgccg ctgatgaaga aacgaaagtg gcccccgaaa cgcttgtgga
     1681 ccgggcgaaa cagcagaggg cgcaggccaa atggcagcca ccgcgccagg cgcaggagac
     1741 cctcgaactg cttctcacgc ttttgcgcgc gcacctggcg gtcaatgccg ttcatgaaca
     1801 attgcagcgc ctggggaata agtaccatac aggtgatgcc ctgtgtgccg agcagcttga
     1861 gcagcgtttc gggattgagc gaccgcgcgt agaggatact gccctcgctg cggaagatcg
     1921 ccaattcgat tgtcatttca aacatatgac ttagaggcag gatcgagagg gcacgatcgc
     1981 tggactggat tggtaccacc ttgagcgcgg catcgatgtc gctgacgatg ttgccatgac
     2041 tgagcatcac gccctttggt acgccggtcg tacccgaagt aaacaccagc tcggccaggt
     2101 cgtgcgctcc cagcgagggc agcttgacgg cattgtatgc gccagcgggc aaggcatcga
     2161 tatcaatcaa tggcacgagc gactttttta aggaactata ttgtttggcc gtcgtgatga
     2221 gatgcgttgc gccggtggtc tctataatcc ttgcgatgaa atcttcacgc gaactgacat
     2281 cgagaggcac agccaccccc ccaacatgca acaccgccag gaacgccacc atccagttca
     2341 tgctgctcgc cgcccatatc aagaaacgct gccccttcac cgcgtcctgc tcctgcaagt
     2401 agcccgcaaa gcggtggaca cgttctgcca gctcgccgta ggtcaggctg acgacctccc
     2461 ccttctcatc cggctcaagc agcgcaacac tcgtttgt
//
LOCUS       DASZAK010000562         6700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_405676, whole genome shotgun sequence.
ACCESSION   DASZAK010000562 DASZAK010000000
VERSION     DASZAK010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6700
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405676"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VGT44_09855"
     CDS             <1..134
                     /locus_tag="VGT44_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEV2581146.1"
                     /translation="RQHNRCQVCGRPRAYIRKFGLCRICFRERALRGELPGVTKSSW"
     gene            219..614
                     /gene="rpsH"
                     /locus_tag="VGT44_09860"
     CDS             219..614
                     /gene="rpsH"
                     /locus_tag="VGT44_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV2581147.1"
                     /translation="MNMTDPVADMLTRIRNAVAARHTKVSIPASKMKLAIARVLKDEG
                     YIKDIEFLKDTPQGTIRLTLRYVEKQPVLTQLKRVSKPGLRVYTKRASIPRVRGGQGI
                     AILSTPQGLMTGHRAYMQGLGGEVVCYVW"
     gene            640..1215
                     /gene="rplF"
                     /locus_tag="VGT44_09865"
     CDS             640..1215
                     /gene="rplF"
                     /locus_tag="VGT44_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV2581148.1"
                     /translation="MSRIGRAPIAVPPKVQVTWTDENFVTVTGPKGKLSYQIDPEITL
                     KLEEGSLAVSRPSDSKVHKAKHGLYRTLVNNMVEGVTKGYTKQLEIHGVGYRAAKVGD
                     NLVVQVGYSHPVEVQPPQGITLSVDGVDPATKATKISVSGIDKQLVGEVSAQIRRIRK
                     PEPYKGKGIRYAGEAIRRKAGKAGKVGGKKK"
     gene            1212..1427
                     /locus_tag="VGT44_09870"
                     /pseudo
     CDS             1212..1427
                     /locus_tag="VGT44_09870"
                     /inference="COORDINATES: protein motif:HMM:NF013057.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
     gene            1689..1823
                     /locus_tag="VGT44_09875"
                     /pseudo
     CDS             1689..1823
                     /locus_tag="VGT44_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017794725.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
     gene            1835..2332
                     /gene="rpsE"
                     /locus_tag="VGT44_09880"
                     /pseudo
     CDS             1835..2332
                     /gene="rpsE"
                     /locus_tag="VGT44_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
     gene            2563..2748
                     /gene="rpmD"
                     /locus_tag="VGT44_09885"
     CDS             2563..2748
                     /gene="rpmD"
                     /locus_tag="VGT44_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV2581149.1"
                     /translation="MATLRVKYVKSTIGRPKDEKATIRALGFRKLQQTVEHEDHPAIR
                     GMIRKVTHLVRVEEVEQ"
     gene            2745..3200
                     /gene="rplO"
                     /locus_tag="VGT44_09890"
                     /pseudo
     CDS             2745..3200
                     /gene="rplO"
                     /locus_tag="VGT44_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            3357..4694
                     /gene="secY"
                     /locus_tag="VGT44_09895"
     CDS             3357..4694
                     /gene="secY"
                     /locus_tag="VGT44_09895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV2581150.1"
                     /translation="MWERLRSIWTIPDLRKKILFTMAILLIFRVLAHITVPLTNTELK
                     ALNSLFSSTNPNQNLSQLFGLLDVFSGGSLQQFSIVALGVYPYITATIVMQLLMPIIP
                     ALENMNREGEAGRLRFSQITRIVAVPLAFLQAIGSAAIFVKFGVLTNFNLFNGSTWLE
                     SLSILLALTTGTMILVWLGELITENGIGNGISIIIFGGIVSRIPSFIQQGYTASTTGG
                     SGAGIVSIGIFLLIGLITIVGIVYVYQGQRRVPIEYPTKRMVGRGMLVGTAQRTYIPM
                     QVNSAGMIPLIFAQSMLIFPAVLAQYLANSNTSWLAQASQWVATYLINTTLWWYWLIF
                     FLLVVAFTYFYAYVVWQQQNIPENLQKQGAHIPGYRPGETTRRHLDEILRRITLGGAL
                     FLGVVAVLPYFTQVGGNQLLSSAALLIVVGVVLDTVRQIEAQMVMRNYSGFLS"
     gene            4744..5394
                     /locus_tag="VGT44_09900"
     CDS             4744..5394
                     /locus_tag="VGT44_09900"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV2581151.1"
                     /translation="MNIVLIGAQGSGKGTQADLLAEHLGLAHVSSGDLFRKAIEERTE
                     SGLQAKKYIDRGELVPDALTVAMILSRLERPDCARGVILDGFPRTIAQAQALDGGLVE
                     VGKQIDLAVYLEVPREELLARLSGRYICKANQHVYNVNTRPPRVSGVCDIDGSPLYQR
                     SDDTGEAVQRRLDIFFNETIQLLGYYDGQGKLVTVNGNQGIDQVQTDLLAVLRDGR"
     gene            5678..5899
                     /gene="infA"
                     /locus_tag="VGT44_09905"
     CDS             5678..5899
                     /gene="infA"
                     /locus_tag="VGT44_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV2581152.1"
                     /translation="MPKKDEIEVEGKVLEALPNAMFKVEIDEQHQVLAVLSGRIRMNF
                     VRIVPGDKVKVVLSPYDLTRGRITWRVRS"
     gene            6057..6173
                     /gene="rpmJ"
                     /locus_tag="VGT44_09910"
     CDS             6057..6173
                     /gene="rpmJ"
                     /locus_tag="VGT44_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV2581153.1"
                     /translation="MKVRASVKPRCDHCKVIRRHGVVMIICSKDPKHKQRQG"
     gene            6200..6580
                     /gene="rpsM"
                     /locus_tag="VGT44_09915"
     CDS             6200..6580
                     /gene="rpsM"
                     /locus_tag="VGT44_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV2581154.1"
                     /translation="MARIAGVDIPRDKRVEISLTYIFGIGLTSSKKILERTRVNPDTR
                     VKDLTEEEVNRLREVIDREYKVEGDLRREVDMNIKRLIEIGCYRGMRHRRNLPVRGQR
                     TRTNARTRRGPKRTVAGKKKAAKK"
BASE COUNT         1503 a         1751 c         2097 g         1349 t
ORIGIN      
        1 ttcggcagca caaccgctgc caggtctgcg ggcggcctcg cgcctatatt cgcaaattcg
       61 gcctctgtcg catctgcttc cgcgaacgag cattgcgcgg tgagttgccg ggcgttacta
      121 agtcgagctg gtaatcggag gatacctttc acccctcctg acacgaagtg tagccgcgca
      181 gcggcgttgg aggggtgagt agaagaggat aatgtttcat gaacatgact gatcctgtcg
      241 cggatatgtt gactcgtatt cgcaatgcgg ttgcggcgcg gcataccaaa gtctcgatcc
      301 cggcctcaaa aatgaagctg gcgattgcgc gggttctgaa ggatgagggg tatatcaaag
      361 atatcgaatt cctgaaggat actccccagg ggacgatccg cctgacgctg cgctatgtgg
      421 aaaagcagcc cgtcttgacg cagttgaagc gcgtcagcaa gcccggtctg cgggtctata
      481 ccaagcgggc gagcatccct cgcgtgcgcg gcggccaggg catcgccatt ttgtcaacgc
      541 cgcagggatt gatgacaggc caccgcgctt acatgcaagg tctgggcggc gaagtcgtct
      601 gttacgtctg gtaggcagac agtagaagga ggaaagaaga tgtcacgtat tggacgcgct
      661 cctatcgctg tgccgccaaa ggtgcaggtg acctggacgg atgaaaattt tgtaacggtc
      721 acaggcccga aaggaaagct ctcctatcag attgatcctg aaatcacgct caagctggag
      781 gaaggctccc tggctgtgtc gcggccatcg gatagcaagg tgcataaagc aaagcacggc
      841 ctgtatcgca ccctggtcaa taacatggtt gagggtgtaa cgaagggcta cacaaaacaa
      901 ttggagattc acggggtagg ttatcgcgcc gcgaaggtgg gtgataacct ggtggtccag
      961 gtgggctatt cgcacccggt tgaagtgcag ccaccgcaag ggatcacctt gagtgtagac
     1021 ggcgtagacc cggccacgaa agcgacgaag atcagcgtga gcgggatcga caaacaactg
     1081 gtcggcgagg tctcggcgca gattcggcga attcgcaagc ccgagccgta caaaggcaag
     1141 ggtatccgct acgcgggcga ggctatccgt cgcaaggccg gaaaagccgg aaaagtcggt
     1201 ggtaaaaaga aatgattaag aaatttcatg caaataaggc gcgtctagtg cgccatcgcc
     1261 gctcgagaaa tcgcctggcc ggctcggtta cgcgaccacg cttgagtgtc ttccgcagtg
     1321 gcggacacat ctacgcgcag gtcattgacg atacgacggg ccgcaccctg gtggcagcct
     1381 cgtcactgga cgagtcgctg cgcagtttcc aatctgagaa gaaggaaacc tcgtctgcac
     1441 agtccaggaa gaagcaggaa gaagagacgc aagtcgagat ggcggcttcg gctgctccgg
     1501 cgaggggcaa gggcgcaaag gcggagaagg ccccggccag gggcggagcg cagcagcagc
     1561 agaagggcaa gggcaagccg gcccagacca agacggtttt gcctacggcg caaaagggcg
     1621 aaaggaagac acctgttgag gcgctcgcgg ccattgcgga taaccgcaag gtggcgcttg
     1681 cgcgcgaagt tggcaagctg gttgctcaac gcgccaaaga acagggtgtg agccgtgttg
     1741 tctttgatcg cggtggctat gcctatcacg ggcgcgtagc cgcgcttgca gaaggcgctc
     1801 gtgaagtggg cctggatttc taagaggagt aacgatgcca aaattagatg cgtcaaaact
     1861 acaactggaa gagaccgttg tccaggtgcg ccgcgtggcg aaggtggtca agggcggtcg
     1921 ccgtttcagc atccgggcac tggtcgtcgt cggtgatcgc aacggtcatg taggctttgg
     1981 cctgggcaag gccgccgagt ataccgaagc cattcgcaag ggcgtggaag atgccaagaa
     2041 gaacctgatc gaggttcccc tggtgggcgc cactattcca ttcatgataa atcaccgctt
     2101 cggcgcgtcc gaggtgatgc tcaagccagc cgccccaggc accggcgtga tcgccggtgg
     2161 cgcggtgcgc gcagtgatgg aatatgctgg tgtgcgcgat atcttgacaa aggcgctggg
     2221 cagcaccaac cctgccaata cgctcgcggc ctgcatcggc gggctgcgcg agctacgctc
     2281 tgcctctgac gaggcggcgc ggcgcgggaa gacgatagag gaactcgtcg gcaagaaaca
     2341 ggcagagcgc ctggctctcg ctgccgcatc tgctgctgaa caggctgccg ccaatcgtgc
     2401 ggcccgcgaa gaggaagcgc gtgaaactcg ctctacgtcg cgccggggtc cgcgcgatcg
     2461 tagtgagggc ggcgaccgtc gcggaggcgg tgatcgtcgc gatcgccgca ggtagctcgt
     2521 cttagtcaac caggccagta atgccctgca atggagaata caatggcaac actacgtgtg
     2581 aaatatgtga aaagcacgat tgggcgtccc aaagatgaga aggcgaccat tcgcgccctg
     2641 gggttccgca agctccagca gacggttgag catgaggatc atcccgccat tcgcggcatg
     2701 attcggaagg tcacccacct ggtacgggta gaggaagtag agcaatgaaa ctctctgatt
     2761 tgcgaccagc tcctggttcg cacaaaactt caaagcgcgt tggtcgcggt cacggctccg
     2821 gtcgtggaaa aaccgccggt cgcggcacga aaggccagaa agctcgcacc ggcgggtcga
     2881 ttcaccgcgc cttcaatggt ggccagacgc gcctatcgaa gcgtctccca ttcttgcgtg
     2941 gtctgggcaa cagcaattat gccttccgca aagattatac gatcgtcaac ctggatgacc
     3001 tcgaacgttt tgaagcaggt tcgcgagtga cgcccgctga atttgccgct gccgggctga
     3061 tgtcgaggag ggagagcgag ggcctggtga aaatcctggg cgatggcgaa cttgatcacg
     3121 cgctcactgt gcgagcgcac aagttctcgg ccagcgcgcg cgagaagatc gaggcggctg
     3181 gcggcaccgt cgaggtgatc cccggtaagg tgtacgagaa gacgaagaga cgcaaggacg
     3241 aggcgaagaa gacgccggct cagcagaacg cgaatgagtg atttcgcgtt attatattcg
     3301 tatgggcgcg gctgatcgcg tctctacgaa cgttctactc gcgaaaggag actcccatgt
     3361 gggaacgact gcgcagtatc tggaccattc ccgatctacg caaaaagatt ctctttacaa
     3421 tggcgatact gctgatattc cgtgtcctcg cccatatcac cgttccgctg acgaatacgg
     3481 aactcaaagc gctgaatagc ctgttttcgt cgaccaatcc aaatcagaac ctgagtcagt
     3541 tgtttggtct gctggacgtg ttttcaggcg gttcgctgca acagttctcg attgtggcgc
     3601 tgggtgttta tccatatatt acggcgacca tcgtgatgca gttgttgatg ccgatcattc
     3661 cggctctgga aaatatgaac cgcgaaggtg aggccgggcg tctgcgtttc agccagatta
     3721 cgcgcatcgt ggccgttccg ctggccttcc tgcaagcgat cggcagcgcg gcgatcttcg
     3781 tgaaattcgg cgtcctgacc aacttcaacc tgttcaatgg cagtacctgg ctggagtcgc
     3841 tctccatcct gctcgcgctg acgaccggta cgatgattct ggtctggctg ggcgaactga
     3901 ttacggaaaa cggtatcggc aacggtatct ccatcatcat ctttgggggc atcgtgagcc
     3961 gcatcccctc tttcatccag caggggtata cggccagcac gaccggcggt tccggcgctg
     4021 gtatcgtcag tatcggcatt ttcctgttga tcggtctgat tacgattgtg ggcattgtct
     4081 atgtctacca gggacagcgg cgcgtaccaa ttgaataccc gacgaagcgt atggttggac
     4141 gcgggatgct ggtgggaacc gcgcagcgca cctacattcc catgcaggtg aacagcgcag
     4201 gaatgattcc gctgatcttc gcccaatcga tgttgatctt cccggcagtg ctggcgcagt
     4261 acctggcgaa cagcaatacg agctggctgg cgcaggcatc gcagtgggtg gcaacgtacc
     4321 tgatcaatac cacgctctgg tggtactggt tgatcttctt tctgctggtt gtggcgttca
     4381 catactttta cgcctacgtg gtctggcagc aacagaatat accggagaac ctgcaaaagc
     4441 agggtgcgca tataccagga tatcggcctg gtgagacgac gcgcaggcat ctggatgaga
     4501 ttctcaggcg catcacgctg ggaggcgcgc tgttcctggg agtcgtggcc gtcctgccgt
     4561 acttcacaca ggtgggcggc aaccagttgc tcagcagcgc agcgctgctg atcgtggtcg
     4621 gcgtcgtgct ggatacggtc aggcagatcg aagcgcagat ggtgatgcgt aactattccg
     4681 gttttctcag ctagcaatgt agctcaatgg cgagtgacat atgcctaacc acggaggtta
     4741 cacgtgaaca ttgtcctgat tggcgctcag ggttcgggca aggggacgca ggctgacttg
     4801 ctcgctgagc atcttggcct ggcccacgtt tcaagcggcg acctgtttcg gaaagctatt
     4861 gaagagcgga ccgagtctgg tttgcaagcc aagaaataca tcgatcgcgg cgagctggtt
     4921 cctgatgccc tgaccgtcgc catgattctg agccgcctgg agcggcctga ctgcgcgcgg
     4981 ggcgttatcc tggatggctt tccgcgcacc atcgcccagg ctcaggccct tgatggtggc
     5041 ttagtcgagg tcgggaaaca gatcgacctg gcggtgtatc tggaagttcc tcgcgaggaa
     5101 ctgctagcgc ggctctccgg tcgctacatt tgtaaagcca atcagcacgt gtataacgtg
     5161 aatacgcgcc cgcccagggt atccggcgtg tgcgatatcg atggaagtcc gctctaccag
     5221 cgcagtgacg atacagggga ggctgttcag cgacggctgg acatcttctt caacgagact
     5281 attcaactgc tcggctacta tgatggccag ggtaaacttg tgacggtcaa cggcaaccag
     5341 ggcatcgacc aggtgcaaac agacctcctg gcggtattgc gcgatggtcg ataaggcacg
     5401 ttgagatgac tggcgtaaaa atagctattc gggctagaga acgggcatta tcgttactac
     5461 gtgtagcgaa ttccgttcat tatcggtaag cggcgttgca tggcgaccct ggcggttgtc
     5521 atgttgcaag ataccctcgt tgtagagtgg gagaaaatac cctctcgaca agtgagggga
     5581 agtgtgttat aattcacagt tggctgcagc agtgaccaaa ctgctgcacc attttatctc
     5641 ggaattatcc aaatgccgtc gttagagaaa ggatgctatg ccaaagaagg atgaaatcga
     5701 agtagagggt aaagtacttg aagccctccc aaacgccatg ttcaaggttg aaatcgacga
     5761 acaacaccag gtgctggctg ttctctcagg gcgaattcgc atgaattttg tgcgtattgt
     5821 accgggagac aaagttaagg tggtcctctc accttatgac ctgacgcgag gccgcatcac
     5881 atggcgcgtg agatcgtgag gttggtggtt tcctcccttt cactcttcag atccttcgaa
     5941 tgagcatcag ttttcggcaa ccaggccggg tagaaaaaca gtagtgtcat gttgcctgct
     6001 gtggaccctt ccctttctgg ctttttgagt tttacgcgca cctgaggagg gcaatcgtga
     6061 aagttcgtgc atcggtcaag ccgcgctgcg atcactgcaa ggtgatccgt cgtcacggcg
     6121 tcgtcatgat aatctgcagt aaagacccca agcacaagca gaggcagggc taagagcggg
     6181 ttttcagtag gaggaaaaga tggctagaat tgccggcgtt gacattccac gcgataagcg
     6241 agtggaaatt tcgctgacat atatcttcgg gatcggtctg acgagcagca aaaaaatcct
     6301 ggagcgaacc cgagtgaacc cggatacgcg ggtgaaggat ctcacggaag aagaggttaa
     6361 ccgccttcgt gaagtgattg atcgtgaata caaagtcgag ggtgatctgc gccgcgaagt
     6421 ggacatgaac atcaagcggc tgatcgagat cggctgttat cgtggaatgc gccatcgccg
     6481 caatctaccg gtgcgagggc agcgcacacg cacgaacgcg cgaacgcgcc gggggccgaa
     6541 acgcacggtg gccggcaaga agaaggcggc gaagaagtaa gccgcgctcg cgtggcatag
     6601 cctgcctacg ccggctgtcg gcgtgggccg catgaggagg acgcagcaag catggcggag
     6661 aaagctccca agcgaccggt ggggcgacag cgccggcgcg
//
LOCUS       DASZAK010000563         1878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_48533, whole genome shotgun sequence.
ACCESSION   DASZAK010000563 DASZAK010000000
VERSION     DASZAK010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48533"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..135
                     /locus_tag="VGT44_09920"
     CDS             1..135
                     /locus_tag="VGT44_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581155.1"
                     /translation="MEASGSIGTNAEKLVAVAATGVDFISLGALTHSAPNFDVSLEFA
                     "
     gene            325..1518
                     /gene="nadA"
                     /locus_tag="VGT44_09925"
     CDS             325..1518
                     /gene="nadA"
                     /locus_tag="VGT44_09925"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEV2581156.1"
                     /translation="MTIQSACASSKEERILPLLTPTGRARHEQSRRVDSIPLRYAEMD
                     GDELQRRIAAARAELGERLVILGHHYQRDEIIQFADFRGDSFKLAQQAAARPQADYII
                     FCGVHFMAESADILSAPHQQVILPNPAAGCSMADMANIAEVEECWEMLHDILGEVIGE
                     AGIIPVTYMNSAANLKAFCGRNGGVVCTSSNAPAVMRWAFTQGKRVLFFPDEHLGRNT
                     ALKYGIPEAQMFVWDPRDPMASNSADDEIAGARVILWKGYCTTHMRFNVKQIEQARAD
                     YPGIKVLVHPECRREVVEAADLYGSTEYIIQQIEQAPAGCQWAVGTEINLVHRLAKEH
                     PDQLIFCLDPVVCPCSTMYRIHPAYLAWVLESLLEGEVINQVKVDEETARWARVALER
                     MLALK"
     gene            1684..>1878
                     /locus_tag="VGT44_09930"
     CDS             1684..>1878
                     /locus_tag="VGT44_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2581157.1"
                     /translation="MLIVDRYRDSTGQSGEEGMQRPNRKEHLKRVLTLERSRRALSQE
                     EVAEAIGVSTRNYQRWEQGLT"
BASE COUNT          423 a          560 c          532 g          363 t
ORIGIN      
        1 atcgaggcgt ccggcagcat cggcacgaat gccgaaaagc tcgtcgctgt cgccgcgacg
       61 ggcgttgact tcatctcgct tggcgcgctg acgcattccg cgcctaattt cgatgtctcg
      121 ttagaatttg cctgatatga aactcatgct gtacggcgcg ctgatggatt ttcaccaaca
      181 ggatcggtac caatttctca tcctgagcgc agcgaaggat ctcactggcc agaaacccag
      241 atccttcgct gcgctcagga tgacagcact ggtattaatc gtaaaaattc attattgcgt
      301 ctgatttgac aaggagactt ggccatgacc atccaatccg cctgcgcatc gagcaaggaa
      361 gagcgcatcc tgccgctgct tacgccaacc ggtcgcgcgc gccacgaaca gagcaggcgc
      421 gtcgatagca ttcccctgcg atacgccgaa atggacggcg acgagctgca gcggcgcatc
      481 gcggctgcca gagctgaact gggcgagcgg ctcgtgatcc tggggcatca ctaccagcgc
      541 gacgaaatca tccagttcgc cgattttcgc ggcgactcgt tcaaactggc gcagcaggca
      601 gccgcgcgtc cccaggccga ttatatcatc ttctgcggcg tgcatttcat ggccgaaagc
      661 gccgatatcc tgagcgcgcc gcaccagcag gtcatcctgc ccaacccggc ggcaggctgc
      721 tcgatggccg atatggccaa tattgccgag gtggaggaat gctgggagat gctgcacgac
      781 attctgggcg aagtgatcgg tgaagcgggc atcattcccg tcacctacat gaactccgcc
      841 gccaacctca aagccttttg cgggcgcaac ggtggcgtcg tttgcacctc gtccaacgcg
      901 ccagccgtga tgcgctgggc cttcacgcag ggcaagcgcg tcctgttctt ccccgatgaa
      961 cacctgggcc gcaataccgc tctgaaatac ggcatcccgg aggcgcagat gttcgtctgg
     1021 gacccgcgcg acccgatggc ctccaacagc gccgacgatg aaatcgcggg agcgcgcgtg
     1081 atcctctgga agggctactg cacgacacac atgcgcttca acgtcaagca gatcgagcag
     1141 gcgcgcgccg actatcccgg cataaaggtg ctggtgcatc ccgaatgccg ccgcgaagtc
     1201 gtcgaggccg ccgatcttta cggctcgacc gagtacatca tccagcaaat tgagcaggcc
     1261 ccggcgggct gccagtgggc ggtcggcact gagatcaacc tggtgcatcg cctcgccaaa
     1321 gaacacccag accagttgat cttttgcctc gacccggtgg tctgcccctg ctcaaccatg
     1381 taccgcattc accccgccta cctcgcctgg gtgctggaga gcctgctcga aggtgaagtc
     1441 atcaaccagg tgaaggtgga tgaagagacg gcgcggtggg cgcgcgtagc gttggagcgc
     1501 atgttggcct taaagtaggc tattcatcgc catcatcgtc ataaggaccg gccataacat
     1561 accttcctcc tttctgcaaa atgtcatggc ctgattaaag ctgaaaattg aaacaagatg
     1621 aacgacattt ccgcgacaca gttcacgaca gatcgagatg tccactaaaa atggagtcga
     1681 atcatgttga ttgtagacag atatcgagat tcgacgggac aatctggaga ggaaggtatg
     1741 cagcgtccca atcgaaagga acatctcaag cgtgttctta cactagagcg cagccgtcgc
     1801 gcgttatctc aggaggaagt ggctgaagcc ataggagtct ccacgcgaaa ctaccaacgc
     1861 tgggaacagg gcttaacc
//
LOCUS       DASZAK010000564         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_227330, whole genome shotgun sequence.
ACCESSION   DASZAK010000564 DASZAK010000000
VERSION     DASZAK010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227330"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1560
                     /locus_tag="VGT44_09935"
     CDS             <1..>1560
                     /locus_tag="VGT44_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581158.1"
                     /translation="PGVLTPGASVNLSVNVQNKGAITWPSTGSSQVILGYHWLDSSGK
                     PINLALVSSLGQGILPADVPSGGTSSIPIVLNTPILAGTYKLVYDLEQQGTWFSSQGA
                     APLTMTVSITPNLPQTYYFAEGYTGTGTSEYLSLTNPSATAAHISITYVFANASEKTH
                     TYTVSAQTHAVLNINNEVGANQSVGMIVQSNQPFVAERSMYTQKGALAAATDTIGSTQ
                     LSSSWYFAEGNTTAGWNTLLAVLNPSSSVVQLHVTFLWSLRSGNGFLPRERTYIIPAH
                     TRSTIVLNYAVPGLQFGMAISASAAVVVERAEYLTQGPMRGGSAVLGALSPQARWYFG
                     AGSTVAGMSEQLILANPTPDWVTAHISYLTVDGHIIPQTVGIPGYGRVVVSVNAVVKQ
                     ALHSTMIVANGQIIAERQDFFNNVNAAQGSTTVMGVSDDSTSWYFAQDTTSAGTTELL
                     AIANPGPLPTTVQVVYYQAKGAPIIKSYTLQGDTRLTINMMSDAGVNNAVGVAIYATT
                     PIVAEQTTFFH"
BASE COUNT          319 a          483 c          451 g          307 t
ORIGIN      
        1 tgccaggggt attgactccg ggcgcgagcg tcaatctgtc ggtcaatgtg cagaacaagg
       61 gcgcgattac gtggccttcg accggcagct cgcaggtcat actcggctat cactggctcg
      121 atagctccgg caagcccatc aatcttgcgc ttgtcagttc gctcggccag ggcattttgc
      181 ctgccgatgt gccatcgggc gggacatcct cgattccgat tgtgctgaat acgcccatcc
      241 tggcgggaac gtacaagctg gtctatgact tggagcagca ggggacgtgg ttctcatcgc
      301 aaggcgcggc tcctctgacc atgacggtat cgatcacgcc caacctgccg cagacctact
      361 acttcgccga gggctatacc ggcacaggca caagcgagta cctgtcactc accaatcctt
      421 ccgcgacggc ggcgcacatc tcgatcacgt atgtcttcgc caatgcgagc gagaagacgc
      481 atacctatac cgtctctgct cagacacacg ccgtcttgaa catcaataac gaggtgggtg
      541 ccaaccaatc ggttggcatg attgtacaga gcaatcagcc cttcgtagcc gaacgcagca
      601 tgtatacaca gaagggcgcg ctggcggcgg ctacagatac catcggctcg acgcagttga
      661 gtagctcctg gtactttgcc gagggcaaca ccaccgccgg gtggaataca ctgctggctg
      721 ttctcaatcc atcatccagc gttgtccagt tgcatgtcac cttcctatgg agtctgcgca
      781 gtgggaatgg ctttttgccg cgcgaacgca cctatatcat cccggctcac acgcgcagca
      841 ccatcgtgct gaattatgcc gtgccgggcc ttcagttcgg gatggcgatc agcgccagcg
      901 ccgccgtcgt ggtcgagcgc gctgaatacc tgacgcaggg gccgatgcgc ggcggcagcg
      961 ctgtgctggg agcgctctcg ccacaggcgc gctggtactt cggcgcgggc agcaccgtcg
     1021 cgggtatgtc ggagcagttg atcctggcaa atcccacgcc ggactgggta acggctcaca
     1081 tcagctacct gaccgttgac ggacacatta tcccgcagac ggtcggtatc cctggctatg
     1141 gccgcgtggt ggtcagcgtc aacgccgtcg tgaagcaggc tttgcactcg acaatgatcg
     1201 tcgctaacgg ccagatcatc gccgaacgcc aggatttctt caataacgtc aacgccgcgc
     1261 agggcagcac gaccgtgatg ggtgtgagcg atgattcgac aagctggtac ttcgcgcagg
     1321 acaccacgag cgccggtacg actgaattgc ttgccatcgc caatcctggc ccgttgccga
     1381 caacggtgca ggtggtctac taccaggcca aaggcgcgcc catcatcaaa agctataccc
     1441 tgcaaggtga tacgcgcctg accattaaca tgatgagcga tgccggtgtc aacaacgcgg
     1501 tgggcgtcgc catctacgcg accacaccca tcgtggccga gcagacgacg ttcttccatg
//
LOCUS       DASZAK010000565         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_546380, whole genome shotgun sequence.
ACCESSION   DASZAK010000565 DASZAK010000000
VERSION     DASZAK010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546380"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1266
                     /locus_tag="VGT44_09940"
                     /pseudo
     CDS             433..1266
                     /locus_tag="VGT44_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            1324..2031
                     /locus_tag="VGT44_09945"
     CDS             1324..2031
                     /locus_tag="VGT44_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581159.1"
                     /translation="MSESEKRIIAFHEGGHALVAYHLPEADTVNRVTILPRGQSLGVT
                     QFTAEEDRYNYSRETLMARIAVGLGGRVAEELTFGPDRVTTGAENDLQVVTNLARRMV
                     TRWGMSEQVGVVFADYNADGGAGLNMRRVEIDDLPLAPRSLIADADGQLFLNGGDLPL
                     SRRYMFAMSAPDANRATSTSMATLIDSEVHRILHQGYEMARTLLCEHNDQLNKLAAEL
                     MTKEQLDRKQFEALLSQ"
BASE COUNT          470 a          608 c          614 g          515 t
ORIGIN      
        1 ggaaatgtgc tggcggtttc cattcaaaaa aatgacgtaa atggattgct ggcgaaaccg
       61 ttacaacaag ggcaggccgg aacgacccct caggcgactg ttactgccaa tcaacgcgct
      121 gcggattttg ctgcatttag ccgctatgtt ggtgcaggtt ctacctgggc aaatagtcca
      181 ataaccaact cgatcgaccc ctctcgactg gtgtacaccc gtatccctga cagtggtgac
      241 gagacactga tgtccttact cgtgagcaac aatgtcgtgg tgagtacact gccggctcca
      301 cagtctcctc tatgggtctc cctcctctgg agaattgctc ctctcgttat catggctgtg
      361 gtgcttgcta tgattcttat ccctcggagt aatcgacgtt caactcgctc cttagatgag
      421 cgcttctctc agatggggaa aagcaagcct caccgttatg gggtgaaaga ggtacgcgaa
      481 ccacgtcaca cccctgaaaa accaaccggc attagcagat ctgttacgcc ttctaaggca
      541 actgtgcaga aacctgttgg gcctcctgtt accttcaacg acgtggcagg gattgatgag
      601 gtgcgtcaag agctggaaga aattgtgcaa ttcttgcgca gtccggaacg ctataacagc
      661 ctgggtgcgc gcattccacg cggcgcattg ctggttgggc ctcctggaac cggtaagaca
      721 ctcttagcta aagccgttgc gggtgaagct ggtgtgcctt tctttagcat gagtgcctag
      781 gaatttgttg aaatgttcgt gggagtcgga gccagccggg tgcgtgatct ttttaaccag
      841 gcgcgtcaat cggctccatg tgtcatcttc ctggatgaaa ttgatgccgt gggccgcaag
      901 cgcaccgtgc gcgttagtgg caacgacgaa cgggatcaga cgctcaacca gttacttgta
      961 gagcttgatg ggttcaattc acgccaggct gtcgttgtgc tggcggccac caacagagtc
     1021 gacatcctgg acaaggccct actgcgtcct ggccgttttg accgccatat tactgtctcg
     1081 ttgcctgatc gcgccggtcg tcaggccatc ttagcagtac atactcgtcg tacccccttg
     1141 catgaagagg tcagccttga gcgcctggct cgcctgacca ccggtatgag cggagcggat
     1201 ctcgctaatc ttgtcaatga agcggcattg tgcgcggccc gccgtgatat ggagcatgtc
     1261 gatcacgatt gctttgaaga tgcgctggct cgcgtgcaac ttggcgcatt gcgccctatc
     1321 gtgatgagcg agagcgaaaa gcgcatcatc gctttccatg aagggggtca cgcgcttgtc
     1381 gcatatcatt tacccgaggc cgatactgtc aatcgtgtga ccattctacc gcgcgggcag
     1441 agtctcggcg ttacccaatt caccgccgag gaagaccgtt ataactatag ccgtgagact
     1501 ctgatggcac gtatcgccgt aggattggga ggccgtgtcg cagaagaact gacgtttggc
     1561 cctgatcgtg tgacaacagg cgcggagaac gatctgcaag tagtgacgaa tctcgcccgg
     1621 cgtatggtga cgcgctgggg catgagcgag caggtgggtg tggtcttcgc cgattacaat
     1681 gccgatggtg gtgccggcct gaatatgcgt cgtgtcgaga tcgatgatct gcctttagcg
     1741 cctcgttcgc ttattgctga tgccgatggc caactattcc tgaacggtgg tgatctgcca
     1801 ctgtctaggc gatatatgtt cgccatgtcc gcccctgatg caaaccgtgc taccagtaca
     1861 tccatggcta cgctgatcga tagcgaggta catcgcatcc ttcaccaggg atatgaaatg
     1921 gcccgaaccc tcttgtgcga gcacaatgat caactcaaca agcttgccgc tgaactcatg
     1981 acaaaggagc aactcgaccg caaacagttc gaggcactgc tctcgcagtg atgatccaga
     2041 caaattgtgg ccagcttcac gatacccgta ggggcgacct tcatggccgc ccgctgtggg
     2101 tcgcctgacg acgctggtcc atcgctgaac cgtccccatc cctcccgggc tatacccgta
     2161 ggggcgacct tcatggtcgc ccaataggca tcaaccgaag ttgcctc
//
LOCUS       DASZAK010000566         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_654215, whole genome shotgun sequence.
ACCESSION   DASZAK010000566 DASZAK010000000
VERSION     DASZAK010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654215"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VGT44_09950"
     CDS             <1..811
                     /locus_tag="VGT44_09950"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2581160.1"
                     /translation="GGADATVQVWDARTGALFLTYRGHHSAVLDIAWSPDGRRIASVS
                     KDNTTQVWDARTGKLLFLYRNQNPSGAVYSVAWSPDSSRIVIGERNSTGGIAQIWNVQ
                     TGKLLLIYQTNVSEAVLHVAWSPNSERIASSSGSMVEVWNAITGKLIFIYEGHNDLVS
                     DIAWSPDGATIATSSYDRTAQVWNARTGQLLYTYPDHTDFVYGLAWSPDGTTIASGSD
                     DGTIQVWNAFLGQHVMTLLDQWRINSLAWFPHGPLVATANNDGLVTVWVMA"
     gene            complement(924..2387)
                     /locus_tag="VGT44_09955"
     CDS             complement(924..2387)
                     /locus_tag="VGT44_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEV2581161.1"
                     /translation="MTAPGEVGIIGIDLGTSSAKVLIIDGRGKTLGAGRADYAVLTPQ
                     PGWAESDPAAWRQAVVSAVRAAMAQAGQPTIAGIGLSGQMHGVVLADDQGRALRPALL
                     WPDSRAGAELEGYRALPEDALQRLANPLAPGMAGPLLCWLARHEAQIYHAARRALQPK
                     DWLRMCLTGAAAGAATDPSDASATLLYDLPADGWADDVIAALGLRRDLFPPILPSGAS
                     AGTLSTTAAGALGLPVGLPIATGAADTAAAALGAGLLSPGPIQLTLGTGAQLIQICAE
                     PLIDATLRTHLYRAADGVNWFRMAAVQNAGLALDWVRRVLAATWDELYASAGNEMLAP
                     GADGLLFLPYLTPERFVHDQHGGAFLGLRIDHGREHLLRAALEGVAFGIRTALEMLPG
                     ASAVNTLRLVGGGSAHPAWRQLLADVLGRELLVVDASDASARGAVLLGGIAAGVWADT
                     AATAALAPGVSAVVTPDAGRARLYDEVYGLWGHDISL"
     gene            complement(2387..>3523)
                     /gene="hisD"
                     /locus_tag="VGT44_09960"
     CDS             complement(2387..>3523)
                     /gene="hisD"
                     /locus_tag="VGT44_09960"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEV2581162.1"
                     /translation="LTPDSLRVSREEIERAHQALDPAVRAAIEHAIRNVRTFHEQQMP
                     HEQWMTEVEPGVMAGEKITPISSVGLYVPRGKGAFPSVMYMLATPASIAGVPRIVVCT
                     PPGPGGSIDDASLVAADLCGVHEIYRVGGAQAIAALAYGTSTIARIRKITGPGSGFVA
                     AAKRLLYGVVDVGLPAGPSESITLADNSADPELLARDLLIESEHGPDSSGLLVTDSRA
                     IVDAVLELLPAKISALPEPRQSFVRTVLEASQGTGGIVLAASMQEAVDFVNEYAPEHL
                     EVQVREPFALLSDLKNAGEILIGPYTPFCTGNYCVGTNACLPTGGFAHTYSCTTVFDF
                     LKRTGIAYVTAQGYASLSGTTRTLADFEGFPAHANAVTDRRVRS"
BASE COUNT          672 a         1217 c         1069 g          565 t
ORIGIN      
        1 tggcggcgcg gacgcgacgg tgcaggtctg ggatgcgcgc accggcgcgc tcttcctgac
       61 ctatcgcggc catcacagcg ccgtgctaga catcgcctgg tcgcccgacg gcaggcgcat
      121 cgcatctgtg agcaaggaca acaccacgca ggtctgggac gcgcgcaccg gaaaactgct
      181 cttcctctat cgcaatcaga acccctctgg cgcggtctac agtgtggcat ggtcgcccga
      241 tagctcgcgc atcgtcatcg gcgaaaggaa ctccacgggt ggtatagccc agatatggaa
      301 tgtgcagact ggcaagctct tgctcatcta tcaaacaaat gtgagcgaag ctgtgttgca
      361 cgtggcatgg tcgcccaaca gcgagcgcat cgcatcaagc agcggcagta tggtagaagt
      421 ctggaacgcc ataacgggga agctcatctt catctacgaa ggccacaacg acctcgtcag
      481 cgacatcgcc tggtcgcccg acggcgccac catcgccaca tcgagctacg acagaacggc
      541 gcaggtctgg aatgctcgca ccggtcagct gctttacacc taccccgacc ataccgattt
      601 cgtctatggc ctggcctggt cgcccgacgg caccaccatc gcctccggca gcgacgacgg
      661 caccatccag gtctggaacg ccttcctggg ccagcacgtc atgaccctgc tggaccagtg
      721 gcgaatcaac agcctcgcct ggttcccaca cggccctctc gtcgccaccg ccaacaacga
      781 cggcctggtg acagtttggg tgatggcata aacgcccaaa gcatccttgt accctaaacg
      841 accgtgtgca aaccccttgc ggtcgccctc gtcctacccg ctccggtggc cgtattaatt
      901 ttgccaatct gcataaccgg ctcctataga gatatatcat gaccccataa tccatacacc
      961 tcatcatata acctcgccct gccggcatca ggcgtaacaa cagccgagac gcctggcgca
     1021 agcgctgcgg tcgccgccgt gtcagcccag acgccagcag caatccctcc aagcagcacc
     1081 gcgccgcgcg ccgatgcatc cgacgcatcg acaaccagca gttcgcggcc cagcacatcg
     1141 gccagcagtt gccgccaggc aggatgcgcg cttccaccac caaccagcct gagcgtgttc
     1201 acagcacttg ccccaggcag catctcaagc gcggttcgaa tgccgaacgc cacaccctca
     1261 agcgcggcgc gcagcaaatg ctcgcgccca tgatcgatgc gcagaccgag aaatgcgccg
     1321 ccgtgttgat cgtgtacaaa cctctccggc gtcagatagg gcagaaagag caacccatca
     1381 gcgccggggg cgagcatttc attcccggcg ctggcgtaca actcgtccca cgtggcagcc
     1441 aggacgcggc gcacccagtc gagggcgagt ccagcgtttt gcacggcagc catgcggaac
     1501 cagttgactc catcggcagc acggtagaga tgcgtgcgca gggtggcatc gataagtggt
     1561 tcggcgcaaa tctggatgag ctgcgcgccc gttcccagcg tcaactggat ggggccgggc
     1621 gacagcaggc cagctccgag agccgccgcc gccgtatccg ccgcgcccgt cgctataggc
     1681 aatcccaccg gcagtcccag cgccccggca gccgtcgtcg agagcgttcc agcagacgcg
     1741 ccggatggca agatcggcgg gaagagatcg cgcctcagcc ccagcgcggc aatcacgtcg
     1801 tccgcccagc catccgccgg gaggtcgtag agcaaggtag cggaggcgtc gctggggtcg
     1861 gttgccgcgc cagcagcagc gccggtgaga cacatgcgca gccagtcctt cggctgcagc
     1921 gcccggcgcg ccgcgtggta gatctgcgcc tcgtgacgcg ccagccagca cagcagtgga
     1981 ccagccatgc ctggcgcgag cgggtttgcc aggcgctgta gggcatcttc gggtagagcg
     2041 cggtagcctt ccaactcagc cccggcgcgc gagtcaggcc agagcagcgc gggacgcagc
     2101 gcccgcccct gatcgtcagc cagcacgacg ccgtgcatct gaccggagag gccaatgccg
     2161 gcgattgtcg gctgccccgc ttgcgccatc gctgcgcgca cggcggagac aaccgcctgc
     2221 cgccacgcgg cgggatcgct ctccgcccag cccggctgcg gtgtcaagac ggcatagtcc
     2281 gctctgcccg cgccgagcgt cttcccacgt ccatcgatga tgaggacttt ggccgagctg
     2341 gtacccaggt cgatgccgat aatgccgact tctcccggcg cggtcattac gacctgaccc
     2401 ggcgatccgt caccgcgttg gcgtgggcgg ggaagccctc gaagtcggcc agcgtgcgcg
     2461 ttgtcccgct caggctggcg tagccctgcg ccgttacgta agcaatcccg gtgcgtttga
     2521 gaaaatcgaa gacggtcgtg cacgagtacg tgtgcgcgaa gccgccggtg ggcaggcagg
     2581 cgtttgtgcc aacgcaatag ttgccggtac agaagggcgt atacgggccg atcagaattt
     2641 ccccggcgtt cttcaagtcg gacagcagcg cgaacggctc gcgcacctgc acttcaagat
     2701 gctccggcgc gtactcgttg acgaaatcga ccgcctcctg catactggcc gccagcacga
     2761 tgccgcccgt tccctgcgac gcttcaagaa ccgtgcgcac gaaggactgg cgcggctccg
     2821 gcagcgcgga aatcttcgcc gggagcaatt ccaacactgc atcgacgatg gcgcggctat
     2881 ccgtgaccag cagcccggaa gaatccggcc catgctcgga ctcgatcagc agatcgcgcg
     2941 ccagcaattc ggggtcggcg ctgttatcgg ccagcgtaat cgactcgctt ggtccggcag
     3001 gcagtcccac atcgacgacg ccatatagca ggcgtttagc tgccgcgacg aagccgctgc
     3061 ccggcccggt aatcttgcgt atgcgcgcaa tcgttgaagt gccataggcc agcgccgcga
     3121 tagcctgcgc cccaccgacg cggtagattt catgcacgcc gcacagatct gccgctacca
     3181 gcgaggcatc gtcgatcgag ccacccggcc ccggcggggt gcagacgacg atgcgcggca
     3241 cgcccgcgat gctggcgggc gtggcaagca tgtacatcac cgacgggaac gcacctttgc
     3301 cgcgcggcac gtagaggccg accgaactga tcggcgtaat cttctcgcct gccatcacac
     3361 ccggctcaac ctcagtcatc cactgctcgt gcggcatctg ctgttcgtgg aaggtgcgca
     3421 cgttgcgaat agcgtgttcg atggcggccc gcaccgccgg gtcaagcgct tgatgcgccc
     3481 gctcgatctc ctcccggctc acgcgcaggc tatccggcgt aag
//
LOCUS       DASZAK010000567         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_85634, whole genome shotgun sequence.
ACCESSION   DASZAK010000567 DASZAK010000000
VERSION     DASZAK010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85634"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..587
                     /locus_tag="VGT44_09965"
     CDS             <1..587
                     /locus_tag="VGT44_09965"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2581163.1"
                     /translation="EHKLTPDGLEYTFATNYLAPFLLTELLLDVLKASAPARIVNVSS
                     VAHTKGKIDFADLQGTQRYSFTKAYGQSKLAQVYFTYELADQLKGTGVTVNALHPGAV
                     ASNFNDGMKGIAHIIGAVIYKFIGISVEQGAQTTLYLATSPEVEGVSGKYFSDCKQTP
                     SSRLSYDMAVRQRLWQVSEELIRQNELSRLMSEK"
     gene            724..975
                     /locus_tag="VGT44_09970"
     CDS             724..975
                     /locus_tag="VGT44_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581164.1"
                     /translation="MPTQEERLTIVEYDLKQFKTETIKAYSDMAMELVMVKGLTEDAV
                     KRLASIKTQIDQQFEQVNTRLDRMETVLTQILARLPEKP"
     gene            1206..4649
                     /gene="secA"
                     /locus_tag="VGT44_09975"
     CDS             1206..4649
                     /gene="secA"
                     /locus_tag="VGT44_09975"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV2581165.1"
                     /translation="MQLIGKMLGDPNKRDIRAIQPTIDQINALGPAMGKLSDEELAAK
                     TEEFREQLALYLKGGKVLERELVKMFRGVLDQVEPLAAKVTGARLHEAIGEPRQVIER
                     RRNPEKMLRDNLQDTLSECLESAYENLNTTFNTLRVTAAMDLAEESGKWPDEAKDPQK
                     ATLALLQKIEPLIGEIDEELQEEAFKKAWPKFEEARDSAQKTADPDEGADERLEKLLG
                     DVLRGLQDELVALKAEAMDELLPSIVKRYRTGKTLDDILPEAFAVVREAGWRTIKMRH
                     YDVQLIGGVVLHQGKIAEMRTGEGKTLVATLPAYLNALTGKGVHVITVNDYLAQRDGE
                     WMGQVYKFLGLSVGIIINAVDPLSPDRRAAYNADITYGTNNEFGFDYLRDNMVQSLDQ
                     MAQRDLNYAIVDEVDNILIDEARTPLIISGQDQESTDQYVRFARWAPRLKAETDYTVE
                     EKTRTVILTESGIEKIEQLAGVKNIYDPENVELTRYMENAIKAQVIFHRDRDYIVRDG
                     EVIIVDEFTGRQMIGRRYSEGLHQAIEAKENVKVQHENRTLATITFQNYFRLYNKLAG
                     MTGTALTEAEELYKIYKLEVIVIPTHKPMIRLDQPDLIYRTEEGKFRAVASEIKELNE
                     KGQPVLVGTTSVEISERLSELLDREGIPHNVLNAKQHQREAQIVAQAGRSGAVTIATN
                     MAGRGTDILLGGNPAGYFDAILRKHAEQVDYIRDMPMLDDYDRQEKEEAIREYIENMS
                     EDEKEELFQAKIRECEEDHKYVVELGGLRIIGTERHESRRIDNQLRGRSGRQGDPGSS
                     RFYLSLEDDLMRRFAADRVSKVMEFAGMDEDMPLESGLVSRFIEQAQTRVEGANFDTR
                     KHVVEYDDVIAKQREVIYADRRAVLEHADLHERVLSMIRTEVQRIVDMHIPGTVVNEE
                     VELEHLFTTVEAWVHIPDEVLPENIHAVRKNDLLKKLTELVVDHYEERGRQLDMLIEQ
                     NPGLGIPNIRDLERSYTMQIIDRLWMDHIHAVDTLRNSIGFRAIAQRDPLVEFKNEAF
                     RMFEDLKLTIQQYIVDSLLKLLRDNITIRVQQPAPKRKVAAPRTLRTNADDIARTSGQ
                     AKSDGAADGASRRGGTATASRSNGRAPVAPPRPVPVNKIGRNDLCPCGSGKKYKKCHG
                     A"
     gene            4810..>5210
                     /locus_tag="VGT44_09980"
     CDS             4810..>5210
                     /locus_tag="VGT44_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581166.1"
                     /translation="MSGQEMLNGNGKYAMNGHNNGHSKGKYSMSGPSAAELEANWEGN
                     LRWRGITRPYSGEDVVRLRGSIQIEYTLARLGAERLWNLLSSEPSVAALGALTGNQAV
                     QQVKAGLQAIYLSGWQVAADANLAGQMYPDQ"
BASE COUNT         1340 a         1527 c         1454 g          889 t
ORIGIN      
        1 gcgagcacaa gctcacccct gatgggctag agtacacctt cgcgacgaat tacctggctc
       61 cgttcctact caccgaactg ctgctggatg tgctgaaggc gagcgccccg gcgcgcatcg
      121 tgaatgtgag ttcagtcgcg catactaaag ggaagatcga ctttgctgat ctgcaaggga
      181 cacaaaggta cagcttcacg aaagcctatg gccagtccaa actggcgcag gtctacttta
      241 cctacgaact ggctgaccaa ctcaagggta caggcgtcac ggtcaatgcg ctccatcctg
      301 gtgcggttgc ctccaacttt aacgacggca tgaaaggtat tgcgcacatc atcggggcag
      361 tgatttacaa attcatagga ataagtgtcg agcagggagc gcagacgacg ctctacctgg
      421 caacctcgcc cgaggtcgag ggagtgagcg ggaagtactt ctccgactgc aaacagacac
      481 cgtcttccag gctctcctat gatatggcgg tacgccaacg tctctggcaa gtttccgagg
      541 agttgatccg gcaaaacgag ctttcccgcc tcatgtcaga aaaataagca agagatcgga
      601 tggcgcatca ataaaccagg cgttcagaga acagccctga tacagagaag ctttaaatgg
      661 gcaaatcgcc agtaacatgc tataatagca ccagtcattt agacagcgaa aggcaagctt
      721 caaatgccga cacaagaaga acgattgact attgtcgaat acgatctcaa acaatttaaa
      781 actgagacca tcaaagctta cagtgatatg gcaatggagc ttgtcatggt caaaggattg
      841 accgaagacg cagtgaagag gctcgcctca atcaaaacgc agatagatca gcaattcgag
      901 caagtaaaca cacgcctgga ccgaatggag acggtactca ctcaaattct tgcgcgccta
      961 ccagaaaagc cctaaaaagc ataaaaatag tatcccctca aagagaaaac cgagcaagat
     1021 tagccattta aaaaagcgag aacagttagt gtggagcagc ttcagaagcg cctgcaacca
     1081 cattatccgc tagcttacat acgctttaat attcgtggta taatgaatga gatattttcc
     1141 aacctacaaa ccttacatct catagattgc aacatagatg ctgactgcca ggagacgatt
     1201 ttcacatgca actgattgga aagatgctgg gcgacccgaa caagcgagac atacgggcga
     1261 tacagccgac tatagatcaa attaacgcgc ttgggccggc gatggggaag ttgagcgacg
     1321 aagagttggc cgcgaagacg gaggagtttc gcgagcaact ggcgctctac ctcaagggtg
     1381 gcaaggtgct ggaacgcgaa ctggtcaaga tgtttcgcgg ggtgttggac caggttgagc
     1441 cgctggccgc caaagtcacc ggcgcgcgac tgcacgaggc catcggcgag ccgcgccagg
     1501 tcatcgagcg gcggcgcaac ccggagaaga tgctgcgcga caacctgcaa gacacgctct
     1561 cagaatgcct ggaaagcgcg tatgagaacc tgaacaccac cttcaacacg ctgcgcgtca
     1621 cggcggccat ggacctggcc gaagagagcg gaaagtggcc cgacgaggct aaagacccgc
     1681 agaaggccac cctggccctg ctgcaaaaga tcgagccgct tattggagag atcgatgagg
     1741 aactccagga agaagccttc aagaaggcat ggccaaaatt tgaagaggcg cgcgacagcg
     1801 cacagaaaac cgccgacccc gacgagggcg cggacgaacg gctggagaag ttgctcggcg
     1861 atgtcctgcg cgggttgcaa gatgaactgg tggcactgaa ggccgaggcc atggacgagc
     1921 tgctcccctc catcgtcaaa cgctacagga cgggcaagac gctcgacgat atcctaccgg
     1981 aagcctttgc cgtcgtgcgc gaggccgggt ggcgcaccat caagatgcgc cactacgatg
     2041 tgcagttgat cggcggcgtg gtgctgcacc agggcaagat cgccgagatg cgcaccggcg
     2101 aaggcaaaac gctcgtcgcc acgctgcccg cctacttgaa tgcgctcacc ggcaagggtg
     2161 tgcatgtcat caccgtcaac gattacctgg cgcagcgtga cggcgaatgg atgggacaag
     2221 tctacaagtt cctgggactc tccgtgggca tcatcatcaa cgccgttgac ccgctttcgc
     2281 ctgaccggcg cgccgcctac aacgccgata tcacttatgg caccaacaac gaattcggct
     2341 tcgactacct gcgcgacaac atggtgcaat cgctcgatca gatggcgcag cgcgacctga
     2401 actacgccat cgtagacgag gtggacaaca tcctgatcga tgaagcgcgc acaccgctca
     2461 tcatctccgg ccaggatcag gaatcgacag accagtatgt gcgtttcgcg cgctgggccc
     2521 cgcgcctcaa agccgaaacc gattataccg tcgaggaaaa gacgcgcacg gtcatactga
     2581 ctgagagcgg catcgagaaa atcgagcagt tggcgggcgt caagaacatc tacgaccccg
     2641 aaaacgttga actgacacgc tatatggaga acgccatcaa agcccaggtg atcttccacc
     2701 gcgaccgcga ctacatcgtg cgcgacggcg aagtcatcat cgtagacgag ttcacgggcc
     2761 gccagatgat cggtcgccgc tacagcgaag gtctgcacca ggcaatcgag gccaaagaaa
     2821 acgtcaaggt gcagcacgag aatcgcacac tggcaaccat caccttccag aactacttcc
     2881 gtttgtacaa caagctggca ggtatgaccg gtaccgcgct gaccgaggca gaagagcttt
     2941 acaaaattta taagctggaa gttatcgtca ttccgaccca caaacccatg atccgcctcg
     3001 atcaacccga cctgatttat cgtaccgagg agggcaagtt ccgcgccgtc gccagcgaaa
     3061 ttaaagaact gaacgagaaa ggccagccgg tgctggtcgg caccacctcg gtcgaaatct
     3121 ccgagcggct ctccgaatta ctggatcgcg aaggcatccc ccacaacgtc ctgaacgcca
     3181 agcagcacca gcgtgaggcg cagatcgtcg ctcaggccgg gcgcagcgga gccgtcacca
     3241 tcgcgaccaa catggccggt cgcggcaccg acatcctgct gggcggcaac cccgccggct
     3301 acttcgacgc cattctgcgc aagcacgccg aacaggtcga ttacattcgc gacatgccca
     3361 tgctggacga ctatgatcgc caggagaaag aagaggccat ccgcgaatac atcgagaata
     3421 tgagcgagga cgaaaaggaa gaactcttcc aggccaaaat tcgcgagtgc gaagaagatc
     3481 ataagtacgt tgtcgagctg ggcggtctgc gcatcatcgg caccgaacgc cacgagtcgc
     3541 gccgtatcga caaccagttg cgcggtcgct ccggacgcca gggggacccc ggctcatcgc
     3601 gcttctacct ctcgctcgaa gacgatctga tgcgccgttt cgcggcagat cgcgtctcga
     3661 aggtcatgga attcgcgggc atggacgaag acatgccgct cgaaagcggg ctggtgtcgc
     3721 gcttcatcga acaggcccag acgcgcgtcg aaggcgcgaa cttcgacacc cgcaaacacg
     3781 tcgttgagta cgacgatgtc atcgccaaac agcgcgaggt gatctacgcc gaccgccgcg
     3841 ccgtgctgga acacgccgac ctgcacgagc gcgtgttgag tatgatccgc acggaggtgc
     3901 agcgcatcgt cgatatgcac atccccggca ccgtcgtcaa cgaggaagta gaactcgagc
     3961 atctcttcac caccgtggaa gcctgggtgc acatccctga cgaggtattg cccgaaaaca
     4021 tccatgccgt gcgcaagaac gatctgctga aaaaactgac cgagctggtc gtcgatcact
     4081 acgaggagcg cggcaggcag ctcgacatgc tgatcgaaca gaaccccggc ctgggcatcc
     4141 ccaacatccg cgacctggaa cgctcgtaca ctatgcagat catcgaccgc ctgtggatgg
     4201 atcacattca cgccgtcgat acgctgcgca acagcattgg cttccgcgcc atcgcccagc
     4261 gcgaccccct ggttgagttc aagaacgaag ccttccgcat gttcgaggac ctcaagctta
     4321 ccatccagca atacatcgtg gacagcctgc tcaagctgct gcgcgacaac atcaccatcc
     4381 gcgttcagca gcccgccccc aaacgcaagg tcgccgctcc tcgcactctg cgcacgaacg
     4441 ctgacgacat cgccagaacc agcggccaag ccaaaagtga cggcgcggcg gatggcgcaa
     4501 gcaggcgcgg cggcaccgcg accgcctctc gctcaaatgg gcgcgcccct gttgccccgc
     4561 ctcgtcctgt cccggtcaac aaaatcgggc gcaacgacct gtgtccctgt ggcagcggaa
     4621 agaagtacaa gaagtgtcac ggggcataag ctccaactta tacatatcct cacaacctta
     4681 aaagccattt cctggtattt ccccttgttc catgttaccc ttgtataaag agcggtggcg
     4741 cgcggaaaca acggcgtttc ccaggataga accaccgatt tgccctggtt agggagaaag
     4801 gtcatcagta tgagcggaca agagatgcta aacgggaacg ggaaatacgc catgaatggt
     4861 cacaacaacg gacatagcaa aggcaaatat tcgatgagcg gcccctcggc ggcggaactg
     4921 gaagcgaact gggaaggcaa cctgcgctgg cgcggcatca cccgccccta cagtggcgaa
     4981 gatgtcgtgc ggctgcgcgg ctcgatccag attgaataca cccttgcgcg gctgggagcc
     5041 gagcgattat ggaatctgct ttcctccgaa ccatccgtgg ccgcgttggg agcgttgacg
     5101 ggcaaccagg ctgtacagca agtcaaggcc ggcttgcagg ccatctatct aagcggctgg
     5161 caggtcgcgg cggacgccaa ccttgccggg cagatgtacc ccgaccagag
//
LOCUS       DASZAK010000568         5442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_297531, whole genome shotgun sequence.
ACCESSION   DASZAK010000568 DASZAK010000000
VERSION     DASZAK010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297531"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            609..2120
                     /locus_tag="VGT44_09985"
     CDS             609..2120
                     /locus_tag="VGT44_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2581167.1"
                     /translation="MQAPPRVLTLDQLRSPTQPANSAEIAGTVDAARQPLALLNQMLR
                     SLAPTDAPDIIARRLVALARQAIGMDLCVVTLVDAEDGVTGGSGMNASARGGRRLSIR
                     ASAPDVNARILDEPPKVIDPVLWQKLRQPTLAGQLPALDALEREALNPLRNVTYSSLL
                     IVPLVAGTECVGLLCGYNSSARDLTEQELLVLQTISSYAAMSIASSALLDYARTGTPV
                     QAFFDEVLADNQPHEDVLRGRANALGYDLTQPSAMLMLEIGASSVDGKEPGEGGAGGE
                     RQLKSRRVLASVHARVKEQYPRSLIHAREHRLYCIVALERDDNAARLDSWLTALMQEI
                     ESDYDTRLCAGIGCVCTDLRDYARGFAEALEALRIGECLRGHVASARFNQLGPYRYLS
                     PFARDRHLSDTHLNHIETIARYDREHKRGNLLLSLETYLTLGSNIKDASELLKVHRNT
                     LTQRLERIQSLCGVNLDDHRECFALQMAIMIYRLRSHLTPGGRRHQDVARLQD"
     gene            complement(2058..2555)
                     /locus_tag="VGT44_09990"
     CDS             complement(2058..2555)
                     /locus_tag="VGT44_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581168.1"
                     /translation="MQRVSLTKIISSFHDAQQPYEEIRHYSLDELLPPGQTLALNVLL
                     GTLSLIANDSPYPRMVAEQQFTSSELSLLVPLLNSHPHYCPYEVLLASFNHRVITEVT
                     VDRCRKRLHEAQLEGIWDQEMRPVRNVLSRTRLKMRSFSIEIASILETGYVLMTPPAR
                     RQMGA"
     gene            complement(3150..3680)
                     /locus_tag="VGT44_09995"
     CDS             complement(3150..3680)
                     /locus_tag="VGT44_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581169.1"
                     /translation="MQMVCQPRGTTAASVLDKSRDDEVMRYFTLDGALPDGRALVLNA
                     QFATLSLISNGDERPRLVAQQQFTASELSLLLPLLELYPHYCPYEAMFASFYNGAVTE
                     ETVERCRRQLQDALDAGVWDQQMRPVRNVLSRVRFKMRSFGIDIVSILETGYILMMKT
                     PIEQVEPTLPLRASAG"
     gene            3826..>5442
                     /locus_tag="VGT44_10000"
     CDS             3826..>5442
                     /locus_tag="VGT44_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEV2581170.1"
                     /translation="MDPPDGKHSPQPEPEPRTRLTQARVRMKLSQKQVAERIGTTHVN
                     VSRWERGITKPGSYFRRRLVQLFGRTEEELDLSRTSDSLPSLSPSPSSTSSSSVPADS
                     IIDPTIPLLPTNPLVGRGEMLAKLKQRLRSGGNVTLTALNGLPGVGKTTLAITIAHDA
                     DIQAHFRDGILWAGLGPQPNLSGIAGHWAGLLGISQSEIEAASDAASWESWAIAMRRA
                     IGSRRMLLVIDDAWKDVDPLLLKVGGPNCAHLVTTRYPSIANQLGIKSAFAIRELGEE
                     DSVQLLRILAPQLMEYDSQRAHELAHAVGGLPLALTLAGNYLRRHTYSGQPRRVEAAL
                     TRLSSAVERLSISEPHSATERHPSLPLDTPISLQSVIAVTDAQLKVEQQAALRALSVF
                     PPKPDSFSEEAALYVADCSTDTLDYLVDTGLLEWSEHGRYTMHQTIADYARSRLDNDS
                     IPSARLADYMSDFVPVHRKDYDLLDRESSAILVALEAAHNLGKGEALMRTIYAYIPYL
                     LARSQYYQAETHLRRAYDAADRKGDTYGKAG"
BASE COUNT         1181 a         1620 c         1511 g         1130 t
ORIGIN      
        1 agcgcgtctc tcaatatgga cacctggtag catgtaaata ccctgatcgt aggagccggt
       61 ttaccgcgct catcgccgat ttatcggcca gtcctggttg atagtggcat tatataggtc
      121 gataatgaac ttgacgacca ggattgaaca tctccgtagg agccgattta tcgcgctcgc
      181 agccgattta tcggcgcgaa tccccctgtc actggattgg actggccgat aaatcggctg
      241 cgagcgcgat aaatcggctc ctacggagat attaccgatg ttgtttctga agcttcataa
      301 tcggcgttga gcgcggcaaa ccggctccaa caggggttgt acctacttgt cgtataagat
      361 ggcttttcct gataccggtc ttatacctgt cttctaattc tgggtgtaga attacttatt
      421 agcaaacaat caggcaggat tggctgattg gtctgatttt actggatttt cgggcagaaa
      481 tatggtacag ttggttttgg ctctgtaaaa gcgccaggac gcaccagcgc gtatttcgca
      541 catcccgtag cacatatgag acatgttaga caggccgatt ttttgcctgt ttacgggagt
      601 ttacacacat gcaagcgcct ccacgagtat tgacactaga tcagttacgc tctccaacgc
      661 agcctgcaaa ctcggctgag attgctggta ctgttgatgc cgccaggcaa ccactggcat
      721 tgctaaatca gatgttgcgc tcgctcgcac cgacagatgc gccagacatc atcgcgcgca
      781 ggctggtggc gctggcaaga caggcgatcg gcatggattt gtgcgtggtg acgctcgtcg
      841 atgcagagga tggggtgaca ggcggttctg gcatgaacgc tagcgctcgc ggcggcagac
      901 gcctgtcgat cagggcatca gctcccgacg tgaacgcgcg cattctcgac gagccgccga
      961 aagttatcga cccggttctg tggcagaagc tgcgccagcc aacgttagca ggccagttgc
     1021 ccgcgctgga tgcgctcgaa cgtgaggcat taaacccgct gcgcaacgtc acatatagct
     1081 cactcttgat tgtgccgcta gtggcaggaa cggagtgcgt ggggctattg tgcggctata
     1141 acagcagcgc gcgcgacctg acagagcagg aattgctcgt cttgcagacc atcagcagtt
     1201 acgcggccat gtccatcgcc agcagcgcgc tgctcgacta cgcgcgtacc ggcacgccag
     1261 tgcaggcatt cttcgatgaa gttctcgcag acaatcagcc gcatgaagat gtgctgcgcg
     1321 ggcgagccaa cgcactgggc tacgacttga cgcaaccctc cgctatgctc atgctggaaa
     1381 taggggcatc ctcagtggat gggaaggaac ctggtgaggg tggggcaggg ggcgaacgac
     1441 agctcaaatc ccgacgtgta ctcgccagcg ttcacgcgcg ggtgaaggag caatacccac
     1501 gttcgctgat tcacgcgcgc gaacatcgcc tgtactgtat cgttgcgctc gaacgtgacg
     1561 acaacgcggc gcgtctcgat agctggctaa ccgcgctgat gcaggagatc gagagcgact
     1621 acgacactcg cctctgcgcc gggatcggct gcgtctgtac agatcttcgc gattatgcgc
     1681 gcggcttcgc cgaggcgctc gaagccctgc gaatcgggga atgcctgcgc ggacacgtcg
     1741 ccagcgcccg cttcaaccag ctcggcccgt accgctatct ctcgcctttc gcgcgcgacc
     1801 ggcatctaag cgatacgcac ctcaatcata tcgaaaccat cgcgcgctac gaccgggaac
     1861 acaagcgcgg caatctccta ctcagcctgg aaacctacct cacgctgggc agcaacatca
     1921 aagatgcctc agaactgctc aaagtgcatc gcaacaccct gacgcagcgc ctggaacgca
     1981 tccaatccct ctgtggcgtc aacctggacg atcacaggga atgcttcgca ttgcagatgg
     2041 cgatcatgat ttatcgatta cgctcccatt tgacgccggg cgggcggcgt catcaggacg
     2101 tagcccgtct ccaggattga cgcaatctcg atgctgaaac tgcgcatctt caggcgggtg
     2161 cgcgataaca cgttgcgcac cgggcgcatc tcctggtccc agataccctc cagctgcgcc
     2221 tcgtgcaaac gcttgcggca tcgatctact gtcacctccg tgatgacacg atgattaaaa
     2281 ctggctaata agacctcgta tggacagtag tgaggatgcg aattgagcaa agggacgagt
     2341 aagctgagtt cgctggaggt gaattgctgc tcagccacca tgcgcggata cggcgaatcg
     2401 ttggcgatca atgacaacgt acccagcagg acgttgaggg ctaaggtctg acccggcggt
     2461 aacaactcat ccagggagta atgtcgtatt tcctcgtatg gctgctgcgc gtcatggaac
     2521 gaagatatga tcttcgtcaa ggatactcgt tgcatagcgc gactccttct acgtttccga
     2581 ccggctgagc gcagctagaa cgcagttcgt tgccggtcgg aataagagag cctagaactt
     2641 gcccgaaacg tgattttcca ctacaaaatt gtgtatactt cacgtttatt gttgctaaag
     2701 gtgtgtttat tgttcctggc aacccgtcct tccgctaatc acttatcggt caatgctaca
     2761 aaactgtgta tcatagacac atttctcgca gaaaatatat ctatggttaa cagtacacaa
     2821 gaaatgtaaa aaattcaaga gttttcagga ggtcttttct caaaaccggt ataagacaca
     2881 gaattttcac aactaatgca aaaccgcgtt ttgcgaagag tagcgttgtg ccccgtaggg
     2941 gcgggggaag atgcgggtca gggtggggac gcttgcgtcg ccctcgtacc cagttatgta
     3001 accatattgt gttctgttag agtgaggacg cttgcgtcgc ctgcgtccct ggtggggcaa
     3061 ccgtatctta tgccaacgaa tcgcccgcgt ccccgatgac gcaacatcat cacgatgcca
     3121 ccagggacgc gggcgacgca agcatcgttt catccggcgg atgctcgtag aggtagggta
     3181 ggttcaactt gctctatcgg cgtcttcatc atcaatatat agccggtttc gaggatggag
     3241 acgatgtcga tgccgaagct acgcatcttg aagcgcacac gggagaggac gttgcgcacc
     3301 gggcgcatct gctgatccca cacacccgca tccagcgcgt cttgcagctg acggcggcag
     3361 cgttccaccg tctcttcggt gacagcgccg ttgtagaagc tggcgaacat ggcttcgtag
     3421 gggcaatagt gggggtatag ttccagcaag ggcaacaaca ggctcagctc gctggccgtg
     3481 aattgctgtt gcgccaccag gcgtggccgc tcatcgccat tggagatcag cgatagtgtc
     3541 gcgaattgcg cgttcaggac gagcgcccgg ccatcaggaa gcgcgccatc cagcgtaaag
     3601 tagcgcatca cttcgtcgtc ccgcgacttg tcgagaacgg atgccgccgt tgttcctcgt
     3661 ggctggcaaa ccatttgcat atcgttgctc ccttatatta ttgctatgca cgatataccc
     3721 gtatcaatat tatgtgcaat acctctgtac ttatgtaaga ggttatgtta tcatttcttt
     3781 taacagattt atcattttct gatagtgatc ggaggaatga tgaagatgga cccgccggat
     3841 ggcaagcatt cgccacagcc agagccggaa cccaggacgc ggctcacaca ggcgcgtgtg
     3901 cgcatgaagc tatcacagaa gcaggtcgct gagcgcatcg gcacaaccca cgtcaatgtc
     3961 agtcgctggg agcgcggcat caccaagccg ggttcctatt tccggcgcag gctcgtgcaa
     4021 ctcttcggca ggaccgaaga agaactcgac ctgtcgcgaa cctctgactc acttccatcc
     4081 ctttcgccct ctccgtcttc cacatcttcc tcgtccgtgc ctgctgactc gatcatcgat
     4141 ccgactatcc cgctcctgcc gaccaacccg ctcgttgggc gcggcgagat gctggcaaag
     4201 ctcaaacaac gcctgcgctc cggcggcaac gtcacactga cggcgctgaa cgggctgccc
     4261 ggcgtgggca agaccacgct ggccatcacc atcgcgcatg atgcagacat ccaggcgcat
     4321 tttcgcgatg gcatcctgtg ggccgggttg gggccgcagc cgaatttgtc cggcatcgcg
     4381 ggtcactggg ccgggctgct cggcatctcg cagtcggaga tcgaagcggc cagcgacgcg
     4441 gcatcgtggg aaagctgggc catcgcgatg cggcgggcta tcggctcgcg acgtatgctg
     4501 ctggtcatcg atgatgcatg gaaagatgtg gacccgctgc tcctcaaggt aggtggccct
     4561 aactgcgcgc acctcgtaac gacacgctac ccctccattg ccaaccagct cggcatcaaa
     4621 agcgccttcg ccatccgcga acttggcgaa gaagatagcg tacaattgct gcgcatccta
     4681 gcgccacaac ttatggaata cgattcgcag cgcgcgcacg aattagcgca cgcggtcggt
     4741 gggctgccgc tggctctcac cctggccggc aactaccttc gccgccacac ttacagcggc
     4801 cagccacgcc gcgtcgaagc cgccctgacg cgcctgagca gcgccgttga acgcctgagc
     4861 atcagcgagc cgcacagcgc gacggaacgt caccccagcc tgccgctcga cacgcctatc
     4921 tcgctgcaat ccgtcatcgc cgtcaccgac gcgcagctca aggtcgagca gcaggccgcc
     4981 ctgcgcgcgc tctccgtctt cccgcccaag cccgacagct tctcggaaga agccgccctc
     5041 tacgttgccg actgctcgac cgatacgctc gactacctcg tagacaccgg cctgctggaa
     5101 tggagcgaac atggccgcta taccatgcat cagaccatcg ccgactacgc ccgctcccgc
     5161 ctggacaacg acagcattcc ttccgcgcgc ctggccgact acatgtccga cttcgtaccc
     5221 gtccaccgca aagactacga cctgctcgat cgggagagca gcgccatcct ggtcgcgctc
     5281 gaagccgccc ataacctggg caaaggcgaa gcattgatgc gcaccatcta tgcatacatc
     5341 ccctacctgc tcgcgcgcag ccagtattac caggccgaaa cgcacctgcg ccgcgcttat
     5401 gacgcagcag atcgcaaagg cgacacctac ggcaaggcgg gc
//
LOCUS       DASZAK010000569         1505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_263630, whole genome shotgun sequence.
ACCESSION   DASZAK010000569 DASZAK010000000
VERSION     DASZAK010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263630"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1083)
                     /locus_tag="VGT44_10005"
     CDS             complement(43..1083)
                     /locus_tag="VGT44_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581171.1"
                     /translation="MIRTHRARRERESRAGEGAAQVVVGVAVAAGKLRAGQTENLPHL
                     GGRPTPPQQIARDPAVHDAPVGLGEALADVPSLHTGLIEFGGNGGGDRIVKRRGRWRR
                     RYQGAGRAQQGFSLQRHNGLRADDLDPGGVAAPCAPRGLLVGEAGESAQVTPVGAGRI
                     ATISRRQELAGGAGQRRFQRRGAETNPGLQMSGAGLQDHAGVMSVGVHGGDGRRPCVI
                     QIDKDVASILVASVGLHIDVTAFAVAHAQEADGSRRGQLFGSPEPFAGERPPSLVVNQ
                     ANQIEVVGHGCQLSANGLQGEKETVVFHDRNIAVETNRRTMNFQRTANCVLTVCLSRG
                     GRRNAEFRHQWR"
     gene            complement(1080..>1505)
                     /locus_tag="VGT44_10010"
     CDS             complement(1080..>1505)
                     /locus_tag="VGT44_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEV2581172.1"
                     /translation="SRRVIGWALDRRVEDDLTLAALRMALELRRPPAGCVHHSDRGSQ
                     YASGDYTDLLKSQGCEISMSHKSSPWENAGCESWMKTLKYEEVYRQEYRDLAEARASI
                     ARFIDEVYNQKRLHSALGYRPPVEFEQALGASNRMEARL"
BASE COUNT          249 a          549 c          419 g          288 t
ORIGIN      
        1 acgaagacgg tcaccggccc tggcgagaag acaagagatg aatcacctcc actggtgccg
       61 aaactcagcg ttgcgcctgc cccctcggct gagacaaaca gttaagacac agtttgccgt
      121 ccgctgaaag ttcattgtac ggcggttggt ttcgaccgca atattgcggt catggaagac
      181 cacggtttct ttctcacctt gcaagccatt cgcagacagc tggcagccat gccccacgac
      241 ctctatctgg ttcgcctgat tcaccaccag actcggcggg cgttccccgg cgaacggctc
      301 tggactgccg aacagttgtc ctcggcggct accgtccgct tcctgcgcgt gcgcaactgc
      361 gaaggctgtg acgtctatgt gcagccctac gctggcaacc agaatgctgg ctacatcctt
      421 gtcgatctgg atcacgcacg gccgacgacc atcgccacca tgcacgccca cggacatgac
      481 ccctgcgtgg tcttgcagac cagccccgct catctgcaag cctggattcg tctcagcgcc
      541 tcgccgctgg aaccggcgct ggccagcgcc gccggcaagc tcttggcggc ggcttatggt
      601 ggcgatccgg ccagcaccga ctggcgtcac ctgggcagac tcgccggctt caccaaccag
      661 aagcccgcgc ggcgcacatg gggcggctac gccccctggg tcaaggtcgt cagcgcgcaa
      721 gccgttgtgg cgctgcaagc tgaagccctg ctgcgctcgg cccgcgcctt ggtatcgccg
      781 tctccagcgc ccacgacgtt tcacgattct gtccccgccg ccgttgccgc cgaactctat
      841 caggcctgta tggagcgatg gcacatccgc caacgcttcc cccaacccga ctggagcatc
      901 gtggactgct gggtcgcgcg ctatttgctg cggcggcgtt ggccgcccgc ccaagtgcgg
      961 gagattctcc gtctggccag cccgcagttt ccccgccgcc acagcgaccc cgacgactac
     1021 ctgcgccgca ccctcgcccg cgcggctttc cctttccctt ctcgccctgt gtgtgcggat
     1081 cacaagcgcg cctccatccg gttcgacgcg cccagtgcct gttcgaactc caccggcggc
     1141 cgatacccca acgccgagtg cagccgcttc tggttataaa cttcgtcgat gaatcgtgcg
     1201 atggaagccc gcgcctccgc cagatcccgg tattcctgcc ggtacacctc ttcgtatttc
     1261 agggttttca tccacgactc gcagcccgca ttttcccaag ggctcgattt gtggctcatg
     1321 ctgatttcgc acccctggct cttcaatagg tcggtgtaat ctcccgaggc atactggctg
     1381 ccacgatcgg aatgatgcac gcagcccgcc ggcgggcgcc gcaactccaa ggccatgcgc
     1441 agcgctgcca gggtgaggtc gtcctccacc ctccggtcca gcgcccagcc gatcacacgc
     1501 cgcga
//
LOCUS       DASZAK010000570         4275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_190304, whole genome shotgun sequence.
ACCESSION   DASZAK010000570 DASZAK010000000
VERSION     DASZAK010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190304"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VGT44_10015"
     CDS             <1..289
                     /locus_tag="VGT44_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2581173.1"
                     /translation="SEEMAAIGQWDRKDQPTRGAAELVEGLQKTFAFVQECLARYTPA
                     DLDYTFSETYQGEDYTFSRQWVIWHVIEHDLHHGGEVSLTLGIHQLAAPDL"
     gene            400..1653
                     /locus_tag="VGT44_10020"
     CDS             400..1653
                     /locus_tag="VGT44_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581174.1"
                     /translation="MSSWTIPQPGPNIADADKPVVVFQSDADFNAVFAAMQAQQLARQ
                     VHTAVALGWSLSELLGRAFLLKDDAVPNDPAWNGAQLIALPEADSAHEKVRALMEHIL
                     FLADVLDVRAIPIDSDAAPTEGATYADVLRELVKKLSTGRFDVGNGEMQQSVLGSINE
                     RLFYWDYKIHYVLQNNPMVVHRAYLVGQSLGALRWYYRSERSALDQETLDKVCHEYIP
                     MLGPYLPPFATGALSNSVEVWGKAALTGQVMQDNSPYSPVELHNQAHIWYDILTGSRR
                     PLSYVDNATHGARYMLRVARVAWPLFVFPLVALLLILVVALVVILANFDQVVKAVAAA
                     ATLVALVSTSHLATSNITVFVERALSHSSDVFKGSPIESLWQSTQQKAVNDATCILPL
                     TPPATLVTPASANSLPQQQQVVKSA"
     gene            complement(1815..2021)
                     /locus_tag="VGT44_10025"
     CDS             complement(1815..2021)
                     /locus_tag="VGT44_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581175.1"
                     /translation="MSSANGTPETKLNRALELVGGSIDPEIAETYPSLAERILAQALE
                     NVELAEQRLREIQKLVGDRDAVLV"
     gene            complement(2452..2874)
                     /locus_tag="VGT44_10030"
     CDS             complement(2452..2874)
                     /locus_tag="VGT44_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581176.1"
                     /translation="MAGLILAVFAIGTVLLAACVRPGTVTTSTGGATPTSGGGGSSCA
                     NGTVHTLATSFQEPCVNVAKGSSLTVMPVVTTTIHILFNGSYVNGTPQPAKEPGAPIV
                     NALQETSKPVTIGPFTTAGTFHIYCSVHPNMDLVINVK"
     gene            complement(3601..>4275)
                     /locus_tag="VGT44_10035"
     CDS             complement(3601..>4275)
                     /locus_tag="VGT44_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910953.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV2581177.1"
                     /translation="VMKTNGEVLEVDNPSAVPDPDEIEKIEEPWMDISIFTPSRYIGT
                     IMDLVTTRRGTFKEMKYIETERVLLTYSIPLAELITDFYDQLKSRTQGYASLDYTFGG
                     YHEADLVKLDILVNGVPVDALSLILHRDKAYAQGRTLVERLRSLIPRQLFEVPIQAAI
                     GSRIIARETVRAMRKDVLAKCYGGDVTRKRKLLEKQKEGKKRLKMVGNVEIPQEAFMA
                     ILSLGE"
BASE COUNT          932 a         1172 c         1159 g         1012 t
ORIGIN      
        1 tagcgaggag atggcagcca tcggacagtg ggaccgtaag gatcaaccca cgcgcggcgc
       61 tgccgaactg gttgagggat tgcagaaaac tttcgcattc gtgcaggaat gccttgcgcg
      121 ttatacgccc gccgacctgg attacacctt tagcgagaca tatcaaggtg aggattacac
      181 attttcgcgc caatgggtta tctggcatgt gatcgagcat gacctgcatc atggcggcga
      241 agtctcgctg acgcttggca tccaccaatt ggccgcgcct gatctgtaat gttatgaatg
      301 tatgaacccg ttgtaggggc attccattct agcgcccttc gtagtacaat agacccaatt
      361 cgttcccgtg acgcggccat actagaaggg agcaagacta tgtcgagttg gactatccca
      421 cagccggggc caaacatcgc tgatgcagac aagccggtcg tggtttttca atcggacgct
      481 gatttcaacg cggtctttgc cgcgatgcag gcgcagcagc tggcgcggca ggttcacacc
      541 gctgttgcgc tgggctggtc gttatccgag ttgctggggc gcgccttcct gttgaaagac
      601 gatgcagtgc cgaatgaccc ggcgtggaat ggcgcgcaat tgattgcgct ccctgaagct
      661 gactccgcgc acgagaaagt ccgcgcgttg atggaacaca ttctctttct ggccgatgtg
      721 ctggatgttc gcgctatccc aatcgacagt gacgcggctc ccacagaagg cgcgacctat
      781 gctgatgtgt tgcgagagct ggtcaaaaag cttagtaccg gtcgtttcga tgtcggcaat
      841 ggggaaatgc aacaatcggt gctgggcagc atcaatgaac gcctcttcta ctgggattat
      901 aaaattcact atgtccttca aaacaatccg atggttgtgc atagagcata cctggtgggg
      961 cagagcctgg gcgcgctacg ctggtattat cgcagtgaac gcagcgccct cgatcaagag
     1021 acgctcgata aggtctgcca cgagtatatc cccatgcttg gcccttattt gccacccttt
     1081 gccacgggcg cgttgtcgaa tagcgtggaa gtatggggga aggccgccct gactggccag
     1141 gtcatgcagg ataattcgcc ctacagtcct gttgaactcc ataaccaggc gcatatctgg
     1201 tacgatatcc tcaccggatc gcgcaggccg ctctcatatg ttgataatgc gacgcatgga
     1261 gcgcgctata tgttgcgcgt ggcgcgcgta gcatggccgc tctttgtctt cccactggta
     1321 gcgctcttgc tcattctcgt cgtcgcgctg gtggtaattc tcgccaactt cgaccaggtg
     1381 gtcaaagctg tggcggcggc cgctacgctg gtggccttag tcagcacctc tcacctcgcc
     1441 acgagcaata ttaccgtctt tgtcgagcgg gcgctctcgc actccagtga tgtatttaag
     1501 gggtctccca tcgaatcgct ctggcagtcc acgcagcaaa aagccgtcaa tgatgcgacc
     1561 tgcatcctgc cactgacacc gccagcgacg cttgtgactc ctgcgtcggc aaacagcctg
     1621 ccgcagcaac aacaagtggt aaagagcgct tgaatggtgg tgaaaaacgg acaacaaaaa
     1681 gccgcctgaa aaggcgactc tgcatagagg aaatacgcca gtctgcacat gtgaaacaat
     1741 tacctgtaga aagtccctat ctgccatata gataggtaga acggcgggtt gttttcacta
     1801 gggatgtcgc gtgactagac caggacggcg tcgcgatcgc cgaccagctt ttgaatctcg
     1861 cgcaggcgct gctcggccag ttccacattt tccaatgcct gggccaggat gcgttctgca
     1921 agcgagggat acgtttcagc gatttcggga tcaatcgagc cgcctaccag ctccagcgcg
     1981 cgattcagtt tcgtttccgg tgtgccgttt gcgcttgaca tgctttttcc tcttctgtca
     2041 tgcaaaggta aaatccacta tacaatcgac gagactctgt atacttatta gtacctcctg
     2101 gtactctgtc gtatcgcgca tttccacaac tgctcatctg tccatcacat cattgtatac
     2161 gctgtgtaag ggctattggt tacaccagtt tcgtcagcgt cagcggcaac agcggtgaca
     2221 atgagataaa acgtgctaac agttcagctt gacgtatcta tctctataat acagcaaccc
     2281 ttcaaaaatc taaaacatct agaccatgat tcctattccc aaccgcagac attttactta
     2341 tcacaccctt tccggcgcaa acgatgccac tgatattgta agtcgcaggg cgaccgcaag
     2401 ggtctccacc ccactccctt gcggtcgccc tcgtccttct gtcggcaagc gttatttcac
     2461 gttgatgacc agatccatgt tggggtggac tgaacaatag atgtgaaagg tgccagccgt
     2521 tgtaaatggg ccgatggtga cgggtttgct cgtctcttgc aaggcgttga cgatgggagc
     2581 gcccggttcc ttggcgggct gcggggtgcc attcacatag gagccgttaa agagaatgtg
     2641 gatcgtcgtc gtgacgacgg gcataactgt caggctcgac cctttggcca cattgacgca
     2701 cggctcctgg aatgaggttg ccagggtatg cactgtgcca ttcgcgcagc ttgacccgcc
     2761 gccgccgctc gtcggggtag ctcccccggt gctggttgtg acagtccctg gacgaacaca
     2821 ggcggcgagc aatactgtgc cgatagcgaa cacggccagg atcaatcccg ccatacgaag
     2881 attcttccta tacatcgcgt tacttcctct catacaatca gtgcttcgtg tatctcgctt
     2941 ccaggctggc gcacctcatt cggatacgaa atagtagtga cagggcgcgc ctcaaagaat
     3001 atgagacgta tacattctac tgcatactat aggcacaggt gcaagcttct gggaatcggg
     3061 taccagtcgt cttttgcgtc aggcgcgctc ctgatttcga tggatgcaaa atttcaactc
     3121 tatgtaatgg aacgcgcgtt tcttgacttt gctgatacag gatggtatag tttctgtatc
     3181 ccaagcaggg atactcccag ggaacctacc ctggcattaa ggcgaatgcg gcctcgcatt
     3241 ggtcatccga aaggagcttc tatgaaacca atgaagaaac caatacgcct cttcggagta
     3301 gtatttcaaa tcgactagta gggcccgtgc aggcgggctg accgagcctc tgacctgttt
     3361 gaccagtacc tgacactacc gtgactatat cttgattgca tcagaatagc cggtcttacg
     3421 acggcttacg ccgcaggttg gcagcagcat ccagaggggg attacacatg ggcatacctc
     3481 acattctgca atgcccctta aatgatgtaa taaatctcgg ttgagcgtga cacgggagat
     3541 tgtgcagaaa cacacacagg gggcctggat atttcattga tgagtccagg ctcttctgca
     3601 ttactccccc agcgataaga tcgccatgaa cgcctcttgc ggaatctcca cattgcccac
     3661 catcttgagg cgctttttgc cttccttctg cttctccaac agcttacgtt tgcgcgttac
     3721 gtcgccgccg tagcactttg ccagtacatc cttgcgcatg gcgcgcaccg tttcgcgggc
     3781 aatgatgcgc gagccgatag cggcctggat cggcacctcg aagagctggc gcgggatcag
     3841 gctgcgcagt cgttcaacca gtgtgcgtcc ctgcgcgtag gctttgtcgc ggtgcaggat
     3901 cagcgagagc gcatctaccg gcacaccgtt gacgaggatg tcgagcttga ccaggtcggc
     3961 ctcgtgatag ccaccgaagg tgtagtcaag ggaggcatag ccctgtgtgc gcgatttgag
     4021 ctggtcgtag aagtcggtaa tcagttcggc cagcgggatg ctgtacgtga gcagcacgcg
     4081 ctcggtttcg atgtacttca tctccttgaa cgtcccgcgt cgcgtcgtca ccaggtccat
     4141 aatcgtgcca atgtagcgtg aaggggtgaa gatcgaaata tccatccagg gctcttcgat
     4201 cttctcgatc tcgtccgggt cgggaaccgc ggatggatta tcgacctcca gcacctcgcc
     4261 attggtcttc atgac
//
LOCUS       DASZAK010000571         1929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_156688, whole genome shotgun sequence.
ACCESSION   DASZAK010000571 DASZAK010000000
VERSION     DASZAK010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156688"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VGT44_10040"
     CDS             <1..1214
                     /locus_tag="VGT44_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2581178.1"
                     /translation="PTDAVNPAFQALLALANTAIWLSILPIGQILLPTQIAALNAANK
                     FSNLAIATSVGVIAALLTNPIAGALSDRTASRLGRRRPWLIAGTVFSAATLVLMANAS
                     SFFTLVIWWAIFHVAANALLAALSAVVPDQVPVRQRATVSAFVSLSLPLGAVVGALLV
                     TKVVKSTALSYYTFSGILVIVMLIFVLVLRDNPLPKGAAPRFNPIAFLASFWVNPVKY
                     PDFGWAWLTRFLVYLSYFTALGYLLYFLQDAVHYQKAAQGVTTFQIILTGSLLISSII
                     AGMLSDKFGRRKIFVTGAGLVIAVSFLMLAFFQTWTGVQLAAAVLGIGFGAYLGVDIA
                     LITQVLPSANARGKDLGVINIANALPQVVGSATAALVITAFHSYTILFALAALLASLG
                     AVLIRQIKSVR"
     gene            1234..>1929
                     /locus_tag="VGT44_10045"
     CDS             1234..>1929
                     /locus_tag="VGT44_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2581179.1"
                     /translation="MYKKRLTIGLTLLLVVFIFGSTLVVGMLKTRKPANAAGPSDWSM
                     YLHDVARSGFNSAETAINPGSAGSLKPLWSFNEGSVIPTQPMVVNGKIYWGSWDGIEH
                     ATNVNGSQAWTANLGTVTSSTCQTTQGIVSAATVATVTINGTPTSVLFVGGGNTQFYA
                     LNATTGAVIWHTPIGVAPNNVIWSSPVLYNGSIYISTSSLCDNPLMPGQIFRLDAVTG
                     TIQNTFTDVPNGCV"
BASE COUNT          387 a          555 c          494 g          493 t
ORIGIN      
        1 ttcctaccga cgcggtgaat ccggcttttc aagcgttact ggcactggcg aacaccgcca
       61 tctggctcag cattctgccc attggacaaa tcctgctgcc aacccagatc gctgcgctca
      121 atgctgccaa taagttcagc aacctcgcta tagccacttc tgtgggagta atcgcggccc
      181 ttctcacaaa tccaattgct ggtgctttga gcgatcgcac ggcatcccgc ctcgggcgcc
      241 gccgtccctg gctgatcgca ggaacagtat tctccgcggc taccctggtg ctgatggcga
      301 acgcctccag ttttttcacg ctggtgatct ggtgggccat tttccatgtc gctgctaatg
      361 ctctcctggc agcgctctca gcagttgtcc ccgaccaggt accagtgcgc cagcgcgcta
      421 cggtgtcggc gttcgtcagc ctctcattac cgctaggcgc ggtggttgga gcgctgctcg
      481 ttacaaaggt cgtcaaatcg acagcattgt cctactacac tttcagcggc atcctggtaa
      541 ttgtgatgct catcttcgtg ctggtgctgc gtgacaatcc acttccaaag ggcgccgctc
      601 cgcgcttcaa tccaatagct tttcttgcat ccttctgggt gaatcctgta aagtatcctg
      661 attttggctg ggcatggctt acccgctttc tggtatacct gagttacttt acggcgctcg
      721 gttatctctt atatttccta caagacgcgg tgcattacca gaaggcagct caaggagtta
      781 caacctttca aatcattcta actggctcgc tgctgatctc ctccatcata gctggcatgc
      841 tttccgacaa gttcgggcgc cgcaagattt ttgtcaccgg agcaggcctt gttattgccg
      901 tttcgttctt gatgctggcg ttctttcaaa catggacggg cgttcagttg gccgctgccg
      961 tactcggtat cggcttcggg gcttacctcg gcgtcgatat cgcgctgatt acgcaggtgt
     1021 tgccttccgc caatgcgcgc ggaaaggatc tcggggtcat caatatcgcc aacgcattac
     1081 cacaggtggt aggctctgca acagcagccc tggtgattac agcatttcac agctatacga
     1141 tcctctttgc tcttgcagcc cttctggcct cgcttggcgc cgtactgatc cgtcaaatta
     1201 agagcgtgcg atgatagaaa ggttgaaagt gtaatgtata agaaacgtct caccatcggt
     1261 ctgactctcc tgttagtagt ctttatattt ggttctaccc ttgtcgtggg catgttgaaa
     1321 acacgcaagc ccgcaaatgc tgctgggcca agcgactggt cgatgtattt gcacgacgtt
     1381 gcgcgcagtg gatttaatag tgccgaaact gccattaacc cgggttcggc cggcagcctg
     1441 aaaccgctat ggtcattcaa tgaagggagt gttattccca ctcaaccaat ggtcgtgaat
     1501 gggaaaatct actggggctc ctgggatggg atcgaacacg cgaccaatgt caatggttcg
     1561 caagcctgga cagcgaacct ggggacggtc acgtcttcta cgtgccagac cacgcaaggg
     1621 atcgtgagtg cggcaacggt cgccaccgtc acgatcaatg ggaccccaac ctcggtcctc
     1681 ttcgtcggcg gagggaatac ccaattctac gccttgaacg cgaccaccgg ggccgtgatc
     1741 tggcatacac ccatcggggt agcacccaac aatgtgatct ggagttcacc cgtcctctac
     1801 aatggcagta tctacatatc cacctcgtca ctctgtgata atcccttgat gccagggcag
     1861 attttccgcc tggatgcggt caccggtacc attcagaaca cctttaccga tgtgccgaat
     1921 ggctgtgtt
//
LOCUS       DASZAK010000572         3764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_227494, whole genome shotgun sequence.
ACCESSION   DASZAK010000572 DASZAK010000000
VERSION     DASZAK010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227494"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1036
                     /locus_tag="VGT44_10050"
     CDS             170..1036
                     /locus_tag="VGT44_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEV2581180.1"
                     /translation="MAEQAEEKRKCSGKERLIQAAQKLAEERPFDDITIDDIIKVAEL
                     SRPAFYYHFIGGKEELRHLLVQRGLLVEAPVTDVRQAILEAALRVFARAGTSAATLED
                     IASEAGVTRGALTWHFHSKEDLLGGIIKQYGPHFFVRPVVDEIDRELELGIHIDEKEL
                     FCQLAGAFYDGFASQGDLARLAILVTHTHPETARLLAAKIVSGRKRIIEFIQKRQQEG
                     YFCKKIDASLFVQLLAMAFAMRAISQGLNDFLPFGHFSREEFIDQLVSLLLYGIVERN
                     PPQEQTETPVQI"
     gene            1093..2742
                     /locus_tag="VGT44_10055"
     CDS             1093..2742
                     /locus_tag="VGT44_10055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEV2581181.1"
                     /translation="MQVNAPPNRRSGGLGYKWVLAMVVILGVFMSILDQTIVNIAIPR
                     LQTAFGADIHSVQWVLTAYILAQGVATPTAAFFADTIGIKRFYIISLSAFTIGSALCG
                     IAWSLPILITFRVLQGLGGAALFPLSITLLFREFPPQERGMAMGLFGVPALLAPALGP
                     TLGGYLVTYAGWQLIFYINVPIGIIAIILSIILIREYRQDAERRFDFIGFVFVALGLS
                     TLLYAISDASTDGWGSTTVLGFLTVGVVSLAIFVTTEILIGNRGGQPLLDMRLFGNGA
                     FRAGVIANLFVVFSLFGGLFLLPIYLQNIRQLSPFQAGLVLLPQALASMVSVIIGGRL
                     VDRFGVRAIMIPGLLILAVATWQLTYITIYSPYSWIQLMLILRGLALGLTVQPLTVAT
                     LSETKPRDLAQASSLSTVARAVSSSLGIAILATLVQTQSLVHFGHLAEQVTPTSPLGQ
                     LVPLIQAYYVAHGASVPAAYQAGIQLISLFVKEQSFVLALQDAFRVTIFVIGIALIAT
                     FFVRSTRRQARPASIPPDRTQIPATKQEEAEESEQFVAIEA"
     gene            2779..3423
                     /locus_tag="VGT44_10060"
     CDS             2779..3423
                     /locus_tag="VGT44_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV2581182.1"
                     /translation="MRRIIFIPILIFLALAAIGGSIAYLIYDNYTYYNTDDAQVTGQI
                     VNISSPGSGILSSLSIKQGDTVTQGETIGQVTLATTQAAVNLTSPMNGTVLQVPAVLN
                     QAVGPGLEIASVTNLSNLTITAYVDENSINNVSVGQSVDIHIDAYGDTSFTGHVQLIV
                     QAAAGQFSLLPNQDPTSGNFTKVGQRIPVVIVLDGNGGKDVVPGMSAEVTIHLH"
     gene            3471..>3764
                     /locus_tag="VGT44_10065"
     CDS             3471..>3764
                     /locus_tag="VGT44_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581183.1"
                     /translation="MNLLITGGLLAFAIVAILVAVLLGLSDQRSQQANTNGTTTSLPA
                     EAVPTARTSMPAATPFPTPEQPEQVSRPLHRTVPLSQELTLTREKPGQVSQPLA"
BASE COUNT          814 a         1119 c         1002 g          829 t
ORIGIN      
        1 atcggcacct acgatagggc tgcttcataa tcagacttcg tcaaacttca agaaattcct
       61 cttccattgc ccttgacaga acaaaattac ctgactatac tttattcatc atctgtatag
      121 tacaggatga tctactgtat catactagac tggcatacta agacgtaata tggcggaaca
      181 ggctgaagaa aaaagaaaat gctccggcaa agagcgactg atccaggcgg cgcagaagtt
      241 agccgaggaa cggccattcg acgatatcac cattgatgat atcatcaaag tggcagagtt
      301 gtcgcgcccg gcattctact accattttat tggcgggaaa gaggaactgc gccacttact
      361 ggtgcagcgt ggtttgcttg tagaagctcc cgttacggat gtgcgccagg ccattcttga
      421 ggcagcgctg cgcgtcttcg cgcgcgcagg cacatcagcc gcgacattgg aggatatcgc
      481 aagtgaggca ggggtcacgc gcggcgcact gacatggcac tttcacagca aggaagacct
      541 gctcggcggt attatcaagc agtatggccc gcattttttc gtgcgtcccg tcgtcgatga
      601 gatcgaccgt gaactcgaac ttggcataca tatcgacgag aaagaattgt tttgccagct
      661 ggcaggggcc ttctacgatg ggttcgcaag ccagggcgat ctcgcacggc tggcgattct
      721 cgtgacgcat acgcatccag agacggcgcg tttgctcgcg gctaagatcg tttcgggacg
      781 caagcgcatc atcgaattca tccagaaacg ccagcaagag gggtatttct gtaaaaaaat
      841 cgatgccagc cttttcgtgc agttactcgc aatggccttt gcgatgcgcg ctatcagcca
      901 ggggttgaac gattttctgc catttggcca cttctcgcga gaagagttca tcgaccagct
      961 cgtctcgctg ctgctctacg gcatcgttga acgcaacccg ccgcaagaac aaactgaaac
     1021 ccctgtacaa atttgagcta tcgtttactt gatagtattt cctactgaaa ggagtctaac
     1081 cgcgtgcaag tcatgcaagt aaatgcgcct cccaacaggc gcagcggtgg actgggctac
     1141 aagtgggtat tggcgatggt ggttattctc ggcgtgttca tgagtatcct ggatcaaacg
     1201 atcgtcaaca tcgccattcc gcgcctgcaa accgcctttg gcgcggacat ccacagcgtg
     1261 cagtgggtgt tgacggccta tattctggcc cagggtgtcg ccacgccaac ggcggctttt
     1321 tttgccgata caatcggcat taaacgcttc tacatcatct cgctctcggc ttttacaatt
     1381 ggctcggccc tgtgcgggat agcgtggagc ctgcccatcc tcatcacctt ccgcgtgttg
     1441 caggggttgg gcggcgcagc gctcttccca ctctcgatca ccctgctctt ccgcgaattc
     1501 ccgccacagg aacgcggcat ggcgatggga ctcttcggcg tacccgctct gctcgcgccc
     1561 gcgctcggtc cgacgctggg cggctacctg gtcacgtatg ccggttggca gctgatcttc
     1621 tatatcaatg tgccgatcgg gatcatcgcc atcatcctct cgattatcct gattcgagag
     1681 taccgccagg acgcggagcg aaggttcgac ttcattggct tcgtctttgt ggcgcttgga
     1741 ctttcaaccc tgctctacgc catctccgat gccagcaccg acgggtgggg atcgacaacc
     1801 gtactgggct ttctcaccgt cggagtggtt tccctggcaa tctttgtcac gaccgaaatc
     1861 ttgatcggca atcgcggagg ccagccgctg ttggatatgc gactcttcgg caacggcgct
     1921 ttccgggcgg gggttatcgc caacttattc gtggtcttca gccttttcgg cggcctgttc
     1981 ctgctaccga tctacctgca aaacatcagg caactcagcc ccttccaggc cggccttgtc
     2041 ctgctgccgc aagcgctggc gtcgatggtc agcgtcatca ttggcgggcg gctggttgat
     2101 cgtttcggcg ttcgagcaat catgatacca gggctgttga tcctggccgt tgcgacctgg
     2161 cagctgacct acatcaccat ctactcgccc tatagctgga ttcagttgat gctgatcttg
     2221 cgtgggctgg cgctcggcct gacagtccaa cccctgacgg tggcgacgct ctcggagacg
     2281 aagccgcgcg acctggccca ggcttcctcg ctcagcacag tcgctcgcgc ggtctccagc
     2341 tcgctaggca ttgcgatcct cgccacgctg gtgcagacgc agagcctggt gcattttgga
     2401 cacctggctg agcaggtcac gcccacttcg ccgcttggcc agctcgtccc gctcatccag
     2461 gcatactatg tcgcgcatgg agcttcagtg ccagcggcct accaggcagg catccaactc
     2521 atcagcctgt tcgtcaaaga acagagcttc gtgctggccc tgcaagacgc tttccgcgtg
     2581 accattttcg tcatcgggat cgccttgatc gccacattct ttgtgcgcag cacacgtcgg
     2641 caggccagac cggccagcat cccgcccgac cggacacaga taccagcgac aaaacaagaa
     2701 gaggccgagg aatccgaaca attcgttgct atcgaagcgt aaaacgcaca cgaatcagaa
     2761 caaaaggaga gttacaacat gcgtcgcatc attttcatcc cgatccttat cttcctggcg
     2821 ctggccgcca tcggaggcag catcgcctac ttgatctacg acaactatac gtactacaat
     2881 accgatgacg cccaggtcac cggccagatc gtcaatatca gctcgcccgg cagcggcatt
     2941 ctcagcagcc tgagcatcaa gcagggcgat accgtcaccc agggtgagac aatcggccag
     3001 gtgacgctgg ccaccacgca agcggcggtc aacctgacca gtccgatgaa tggcacggtc
     3061 ttgcaggttc cagccgtcct caatcaggca gtgggtccag gactggagat tgcctccgtg
     3121 acgaatctta gcaacttgac aattacggct tacgtcgatg aaaattcaat taacaacgtc
     3181 agcgttggtc aatcggtcga tattcacatc gatgcctatg gcgataccag ctttaccgga
     3241 catgtgcaac tgatcgtgca ggcggcggca ggccagttct cactactgcc gaaccaggac
     3301 ccgaccagcg gcaatttcac caaagttggg cagcgcattc ccgttgtgat tgtacttgac
     3361 ggcaatggcg gcaaagatgt cgtcccaggt atgagcgctg aagtcacgat ccacctgcac
     3421 taaacgagcc gcttgatcgt atcacgcgca attaaggagt acacatctct atgaatctct
     3481 tgattaccgg cggcttactg gccttcgcca tcgtcgccat cctggtcgct gtgctgctgg
     3541 gtctaagcga tcagcggtca cagcaagcca atacaaacgg gacgacgact tccctgccgg
     3601 cggaagctgt acccacagcc agaacatcca tgcccgctgc tactccattt ccaacgccgg
     3661 agcagccaga acaggtgagc aggccgctcc acagaacggt tccacttagc caggaactca
     3721 cgctaacgcg cgaaaagcct ggacaggtca gccagcccct ggcg
//
LOCUS       DASZAK010000573         2638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_370310, whole genome shotgun sequence.
ACCESSION   DASZAK010000573 DASZAK010000000
VERSION     DASZAK010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370310"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1321)
                     /locus_tag="VGT44_10070"
     CDS             complement(59..1321)
                     /locus_tag="VGT44_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner-membrane translocator"
                     /protein_id="HEV2581184.1"
                     /translation="MSDTLENTTGGTPTIEATQSVEAASVEVPSYRQRQTVGQLFRTD
                     LGFIPVLATFIVIVIFFQVISGGLFLSSVNITNLFLQTATYGLLGLGIVLVLLLGEID
                     LSIAAVATFSSVIMADLSERAGVPAFVAILAALLTGALIGAINGFFVAVLRIPSFIVT
                     LAANIAFSGLVITFLFGQATLEIANSFIVAIAGSFASYLPDILGVGLPTLAILLYAGY
                     LVLNYIRRRNAGLRNPSLPLFLALVIGPIVVVEGVVIVFENLHGVPYSTALLFALIAL
                     FWIILTKTSFGRHIYAVGGNKEAARRAGINVVGIQIAVFSLCSLLAAVGGIVEASRGN
                     SVASQIPAQLLLDPIAAAVIGGVSLFGGKGSVWSIVLGILIIGSLENGLALQSIPADR
                     VLWIEGGVLLLAVTVDAILRRLQARTGR"
     gene            complement(1325..2164)
                     /locus_tag="VGT44_10075"
     CDS             complement(1325..2164)
                     /locus_tag="VGT44_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2581185.1"
                     /translation="MAENMTPAVGDTSPPPLEPSPRQPRLLMRNISKSFGVVRARSGV
                     DFEVYDNEVVGLVGDNGAGKSTLIKTISGVGPADEGEIFVGGQPVRITNPQIATRLGI
                     QTVYQDLALCDNLDVVANLFLGREERFPWRTLNEVDMEKQGLAVLRSLDVKLPSARAI
                     VSTLSGGQRQSIAVAKSLLRKAQVVLLDEPTAALGVAQTRQVLNMISRLREQGLAVVV
                     ISHNLADVFEVVDRVIVLRLGKRVGTFEVKNTTAEQVVAAITGAEFGKLTEAESNGDT
                     GGK"
     gene            complement(2320..>2638)
                     /locus_tag="VGT44_10080"
     CDS             complement(2320..>2638)
                     /locus_tag="VGT44_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2581186.1"
                     /translation="VTGQDATLTGLQNILLGDQSMTVLKPYKLEAGATASLVAAISNG
                     TDTTSLINGQTANANKVNIPSILETPEAIDKTNVMDVVNTGEVTLAQVCQGLPSKAGG
                     ICP"
BASE COUNT          635 a          715 c          733 g          555 t
ORIGIN      
        1 ccagggcgat ggccagcatc gccctggcgg taatctgatg cattgttaat gtgtaatgtt
       61 agcgaccggt gcgggcttgc agtcggcgca ggatcgcgtc gacggtcacc gcgagcagca
      121 gcacaccacc ttcaatccat agcaccctat cagccgggat actttggagc gccaggccgt
      181 tttccaggct gccaatgatc aaaatgccca gcacgatcga ccagacagag ccttttccgc
      241 cgaagaggct gacgccgccg atcaccgctg ctgcaatggg gtcaagcagc agttgagccg
      301 gaatctggct agcaacggag ttgccgcgcg acgcttcgac aatgccaccg accgctgcca
      361 gcaacgagca gaggctgaat accgcaattt gaatgccaac gacgttgatt ccggcgcggc
      421 gtgctgcctc cttgttgccg cccacagcgt agatatggcg gccaaacgag gtcttggtca
      481 ggatgatcca gaagagcgcg atcaaagcaa agagcagggc cgtcgaatag gggacaccgt
      541 gcaggttttc gaaaacaatc acaactcctt caacaactac gattggtccg atcaccagag
      601 ccaggaataa tggtaaagag ggattgcgca gcccggcatt tctccgtcgt atatagttca
      661 gaacgaggta accggcgtaa agtaaaatag ccagcgttgg caatccaacg ccgagaatat
      721 cgggcaggta gcttgcaaaa gagccggcga ttgcaacaat aaatgaattg gcgatttcta
      781 gcgtcgcctg tccaaagaga aacgtgatga ccagtcccga aaaggcgatg ttggcggcca
      841 gggtcacaat gaacgaaggg atgcgcaaca ccgccacgaa aaagccgttg atcgcgccga
      901 taagcgcgcc ggtcagcagc gccgccagaa tagcgacaaa agcaggaact cctgcccgtt
      961 ccgaaagatc tgccataatg accgaactga aagtggcaac cgcagcgatt gaaaggtcaa
     1021 tttctcccag gagcagcacc agcacaatgc ccaatccgag caggccatag gtggcagttt
     1081 gcaggaaaag gttcgtaata ttaactgaac taaggaaaag gccgccacta atgacttgaa
     1141 agaatatgac gattacaata aaggtggcaa ggacggggat aaatcccagg tcggtgcgga
     1201 acaactggcc tacggtctgg cgctgcctgt acgaaggcac ctctacagat gctgcttcaa
     1261 ccgattgcgt cgcttctatt gtaggggtcc ctcccgtggt attctcaagc gtgtcactca
     1321 tcgattactt tcctcctgta tcgccattgc tttcggcttc cgtaagtttg ccgaattccg
     1381 cgccggtgat agcggcgacg acctgctctg cagtagtgtt tttgacttca aaggtcccaa
     1441 ccctttttcc cagacgtagt acgatcacac gatcaactac ttcaaaaacg tctgccaggt
     1501 tgtgtgatat tacgacgaca gctaatccct gctcgcgcag cctgctgatc atattgagta
     1561 cctggcgcgt ttgcgcaacg cctagcgcgg cggttggctc gtcgagcagc acaacctggg
     1621 ctttgcgcag aagcgacttt gcgaccgcga tagattggcg ttggcctccc gagagggtag
     1681 agacgatggc gcgtgccgag gggagtttga catccagcga gcgcagaacg gccagcccct
     1741 gtttctccat atccacttcg ttcagcgtgc gccacgggaa gcgctcctca cgtccgagaa
     1801 agaggttcgc gacgacatcc aggttgtcgc aaagagcgag gtcctggtac actgtctgaa
     1861 tacccagccg ggtagcgatt tgcggattgg tgattctgac cggctggccc ccgacaaaga
     1921 tttcgccctc gtcagccggt ccaacgccag aaatagtttt aatcagggtt gattttccgg
     1981 ccccgttatc gccaaccagg ccgacaactt cattgtcata aacctcgaaa tcgacgccgg
     2041 accgcgcgcg caccacgccg aaggacttac taatgttgcg catcaataag cgtggttgcc
     2101 ttggggatgg ctccagtggg ggaggcgagg tgtcaccaac cgcaggagtc atgttttcag
     2161 ccataaattc ctccaaatat ccaatgtagg tcacttttca ttgggaatcg cggatgctgg
     2221 attagtatac cacttgcacg gtgaatagtg tgaatttacc tggcaccggt atggtgccag
     2281 gtaaattcat caaaagaaat gcacgccaga tctcaagcgt taggggcaga tacccccggc
     2341 ttttgaaggt agaccctgac aaacctgggc gagggtgact tctccggtat tcaccacgtc
     2401 catgacgttc gtcttatcaa tggcttccgg cgtctccagg atggagggaa tgttcacctt
     2461 gttggcattg gcggtctggc cattgatcag ggaggtggtg tctgtgccgt tgctgatggc
     2521 tgccaccagc gaggcagtcg cgccagcctc aagtttatat ggctttaaga ccgtcattga
     2581 ctgatcgccc aataggatgt tctgaagtcc cgtaagggtt gcatcctggc cggtgacc
//
LOCUS       DASZAK010000574         4859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_297687, whole genome shotgun sequence.
ACCESSION   DASZAK010000574 DASZAK010000000
VERSION     DASZAK010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4859
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297687"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1628
                     /locus_tag="VGT44_10085"
     CDS             117..1628
                     /locus_tag="VGT44_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEV2581187.1"
                     /translation="MAGIEQVRALAEPFAPEQVAAICGIDTPAIRRIARELAAAPRAA
                     VYGRVGTCTQSFGTLVSWLVDVLNALTGNLDREGGAMFPMAAAGGRNTGGTPGRGRGV
                     RFGRWKSRVRGLPEAYSELPASCMAEEIETPGAGQIKALITLAGNPALSTPNSGRLQQ
                     ALDRLSFMVSVDMYLNETTRHANVILPPSPTLTRSHYDLAFYQLSVRNIAHYSPPVFE
                     REPGVMDEWEIYARLAAILAGQEADTDASVIDDLAIMTLIQREIATPGSLVEGRDPGE
                     LIEALRPRVGPDRLLDFLLRSGPYGDGFGKRDGLSLAKLEAHPHGIDLGPLQPRIPEV
                     LRTPSGKIELAPQPLVEDTARLRAAFEQHLSEQHGTNGHMQLIGRRDLRSNNSWMHNL
                     HVLTKGKERCTLHMHPQDAARLGLEEGALAHVASRAGEVQIPVEITDAIMPGVVSIPH
                     GWGHNQPGTHMQIAAAHAGVNTNILAAEDEVDPLSGNAVLNGVEVTVERVASY"
     gene            complement(1661..1954)
                     /locus_tag="VGT44_10090"
     CDS             complement(1661..1954)
                     /locus_tag="VGT44_10090"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV2581188.1"
                     /translation="MNIVLWVAQGLLALAFLLAGFMKATQPVANLKKNMAWVAAVPAA
                     FVRFIGVAELLGAIGLILPALLNIQSWLTVAAAAGLVVVMASASLPSPTPILM"
     gene            2139..2741
                     /locus_tag="VGT44_10095"
     CDS             2139..2741
                     /locus_tag="VGT44_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2581189.1"
                     /translation="MVAPSKGVDPRVKRTRQLLQHAFIELIQEKGIASISIQDITERA
                     TVNRATFYAHFPDKYALMDSILREQFQQRASSMLPSKPQWSLGCLRILIRAVFDFLKE
                     FHHDCKPGDTQFDPMFERAVQQELHTILAGWLMQASLSEKKRRIPVDTVADVLSWAMF
                     GTAVTWSRDGQTPSAEEMTRHVLLILTEGVAHLAPDLLPD"
     gene            complement(2695..3678)
                     /locus_tag="VGT44_10100"
     CDS             complement(2695..3678)
                     /locus_tag="VGT44_10100"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEV2581190.1"
                     /translation="MGRKRYYDDDDEYDDDDEDDDDYDGWNYYRPSKPIKVEGGLKPK
                     SERGQIGSTWWSKRWISVLESFGMGARLTRGRTYARQGQVVSIDVEPGIVNAKVQGSQ
                     IRPYKVKIQLKSLSDQEWEQVLDAMASQAIFAAKLLAGEMPGDIEDAFKAVHLSLFPT
                     KVSDLVTDCTCPDWANPCKHIAAVYYLLADRFDEDPFLIFKLRGRSKEQIIQALRQKR
                     LASLPADDVVPAASDDTPLTDDGEPLEDDLNTFWQVSAALDDFPVNPYASRIDKAILK
                     RLGSAPFTIEGQPLTAILAKAYDAVDDVVRKTNNTTINQAEDQERDAPLPR"
     gene            complement(3694..>4859)
                     /locus_tag="VGT44_10105"
     CDS             complement(3694..>4859)
                     /locus_tag="VGT44_10105"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2581191.1"
                     /translation="AFEAEARRNDVVITTYSLALRDREHLASIEWEYVVVDEAQNIKN
                     EAAKQSQAIKAIKARHKIALTGTPVENRLSELWSIMEFLNPGYLGSPTDFRQQFAIPV
                     ERYHDAERAETLKNVIQPFLLRRLKTDKSIIADLPDKMEMRVYCNLTREQATLYEAVV
                     KEMMEKIEDSEGIERRGLVLATLMKLKQICNHPAQFVGDGSALPGRSGKLARLEEMLE
                     EALAAGDKALVFSQFAEMGGLLRHQLQEVLDCEALFLHGGTPKKMRDSMVQRFQEQGR
                     GAPPIFILSLKAGGVGLNLTAANHVFHFDRWWNPAVENQATDRAFRIGQLKNVQVHKF
                     VCVGTLEERIDAMIEQKKELAESIVGSGESWLTEMSNTELKELFALSREAVGE"
BASE COUNT          962 a         1535 c         1379 g          983 t
ORIGIN      
        1 aggaggccga cgaacaccct ttcatgcggc cagggcgcga cgcctagttc cttttcgccc
       61 tcgttcatac cctcttcgag gaagggctgg tcgcgccggg caggctcgcc gaacatatgg
      121 cgggcatcga acaggtgcgc gccctggccg aaccgttcgc gccggagcag gtcgccgcca
      181 tctgcggcat cgacacgccc gctatccgcc gcatcgcgcg cgaactggcc gccgcgccgc
      241 gcgccgccgt ctatgggcgt gtcggcacct gcacgcaatc cttcggcacg ctggttagct
      301 ggctggtaga cgtgctgaac gccctgaccg gcaacctcga ccgcgaaggc ggcgcgatgt
      361 tcccgatggc cgccgccggt ggccgcaaca cgggcggcac gccgggacgc gggcgcggcg
      421 tgcgcttcgg gcgctggaag agtcgcgtgc gcggactgcc cgaggcctac agcgaactgc
      481 ccgcctcctg catggccgag gagatcgaaa cacccggagc gggccagatc aaagcgctga
      541 ttacgctggc cggtaatccc gccctcagca cgcccaacag cggccgcttg cagcaggccc
      601 tcgataggct cagcttcatg gtcagtgtgg atatgtacct taacgagacg acccgccacg
      661 ccaacgtgat cctgccgcca tcgcccacgc tgacccgctc gcactacgac ctggcctttt
      721 accagctctc cgtccgcaat atcgcgcact attcaccgcc cgtctttgaa cgcgagccgg
      781 gcgtaatgga cgaatgggag atttacgcgc gcctggccgc tatcctcgcc ggacaggagg
      841 cagatactga tgcgagcgtc atcgatgatc tggccatcat gacgctgatc cagcgcgaga
      901 tcgccacccc cggttcgctc gtcgaaggac gtgatccagg tgaactgata gaggcattgc
      961 gccctcgcgt cgggccggac cgcctgctcg acttcctgct gcgcagcggc ccctacggcg
     1021 acggcttcgg caagcgcgac gggctgagcc tggcaaagct ggaagcgcac ccacacggca
     1081 tcgacctggg tccactacag ccgcgcatcc cggaggtact gcgcacgcca tccggcaaaa
     1141 tcgagctggc ccctcaaccg ctggtggaag atacggcccg cctgcgcgcc gcgttcgagc
     1201 agcacctcag cgagcagcac gggacgaacg gacatatgca gttgatcggg cggcgcgacc
     1261 tacgctccaa caactcgtgg atgcacaacc tgcacgtcct gacgaagggc aaggagcgct
     1321 gcaccctgca tatgcacccg caagatgccg cgcgcctggg cctggaggag ggcgcgctgg
     1381 cccacgtcgc ctcgcgcgcc ggtgaggtgc agatccccgt cgagatcacg gacgccatca
     1441 tgcccggcgt ggtcagcatc ccacacggct ggggtcacaa ccagcccggc acccacatgc
     1501 agattgccgc agcccatgcc ggcgtgaata caaacattct ggccgccgag gatgaagttg
     1561 acccgctttc ggggaatgcc gtgctaaacg gcgtcgaggt gacggtggag agagtagcca
     1621 gctactagcg cttaatcttc tccggttgat tcatcaaatt ctacatcagt ataggcgtcg
     1681 gcgagggtaa agaggcgctc gccatgacca cgaccagccc ggcggcggca gccaccgtca
     1741 accatgactg gatattcaag agcgcgggaa gaatgagtcc gatcgcccca agcagctcgg
     1801 ccaccccgat aaagcgcacg aaggcggcgg gtacggcggc cacccaggcc atatttttct
     1861 tcaagttggc aaccggttga gtggccttca taaaaccggc gagtaaaaac gccagcgcca
     1921 gcaagccctg ggctacccat aacacaatgt tcatcgtata gtttcctttc gttctttgcg
     1981 ttgcgacata tgttgtgtaa gcaatggcta ttgcttaatc actaaccgtt gtttataata
     2041 ctactgtagg attgttggat atgcaatgag caaaatcgcg tttttgtagc ttagacaaca
     2101 caatcagaaa agtgttggga aacatgaagg acatggctat ggtcgctcca tcaaaaggcg
     2161 tggacccgcg cgtcaagcgc acccgccagc ttttgcagca cgcttttatt gaactcatcc
     2221 aggagaaggg gattgcatcc atcagcatcc aggatattac ggaacgagca acggtgaatc
     2281 gtgccacctt ttatgcccac ttcccagaca aatatgcctt aatggattcg attctccgcg
     2341 agcaatttca gcaaagggcg agtagcatgc ttccctcgaa acctcaatgg agcctggggt
     2401 gcctgcgcat acttatcagg gcagtcttcg actttctgaa agagtttcat cacgactgca
     2461 agcctggcga cacacagttt gaccccatgt tcgaacgggc tgtgcagcag gaactccata
     2521 ccatactcgc aggctggctc atgcaggcgt cgctatcgga gaagaaacgg cgaattcctg
     2581 tggatacggt ggcagatgtc ttgagttggg ctatgtttgg cactgccgtt acatggagcc
     2641 gcgacggaca aacgccatcg gcggaagaga tgaccaggca cgtactactc atcctcaccg
     2701 agggagtggc gcatctcgct cctgatcttc tgcctgattg atggttgtat tgttggtttt
     2761 cctcactaca tcatccaccg cgtcgtacgc tttcgccagg attgccgtca ggggttggcc
     2821 ttcaatcgtg aaaggggcag agccaagccg cttgaggatg gctttatcga tgcgggaagc
     2881 atacgggttg acgggaaaat catctaaggc ggcggagacc tgccagaacg tgttgaggtc
     2941 gtcttcaagc ggctcgccat cgtcggtcag gggtgtgtca tcacttgcag caggcacgac
     3001 atcatctgct gggagcgagg cgaggcgctt ctggcgtagc gcctggatga tctgctcctt
     3061 ggagcgaccg cgcagtttga agatcaggaa cgggtcttcg tcgaagcggt cggccagcag
     3121 gtagtagacg gcggcgatat gcttgcacgg attggcccag tcggggcagg tacagtcggt
     3181 cacgaggtcg ctgactttcg tcgggaagag tgagagatgc acggctttga aggcatcctc
     3241 gatgtcgcca ggcatttcgc ccgcgagcag cttcgctgcg aagatggcct gcgacgccat
     3301 cgcgtccagc acctgttccc attcctggtc tgaaagcgac ttcaactgga ttttcacttt
     3361 gtatggccgg atttgcgatc cctgcacctt cgcgttgacg atgcccggtt caacatcgat
     3421 cgagaccacc tgcccctgcc gcgcataggt acgacctcgc gtcagccgcg cgcccatgcc
     3481 gaacgactcc agcacgctga tccagcgttt cgaccaccac gtcgagccga tctgaccgcg
     3541 ctcgcttttc ggcttgagac caccctcgac cttgatcggc ttagaaggtc ggtagtaatt
     3601 ccagccatcg taatcatcat catcttcgtc atcatcatcg tactcgtcgt catcgtcgta
     3661 gtatctcttg cgtcccatgt cccatgctcc tctttattcc ccgactgctt cgcggctcaa
     3721 ggcgaacagt tccttgagtt ccgtgttgga catctcggtc agccagcttt cgccgctgcc
     3781 cacgatgctt tcggccagtt ctttcttctg ctcaatcatc gcatcgatgc gttcctccag
     3841 cgtgccgacg cagacgaatt tatgcacctg cacattcttc aactgcccga tgcgaaaagc
     3901 ccgatcggtc gcctggttct ccaccgccgg attccaccac cgatcaaagt gaaagacgtg
     3961 gttggccgcc gtcaggttga gtccgacgcc gcccgccttc aacgacagga tgaagatggg
     4021 cggcgcgccc cgcccctgtt cctggaagcg ctgcaccatg ctgtcgcgca tcttcttggg
     4081 cgtcccaccg tgcagaaaca gcgcctcgca atccaatacc tcttgcaact gatggcgcag
     4141 caggccgccc atctccgcga actgactgaa gaccagcgcc ttgtccccgg cggccagcgc
     4201 ctcctcgagc atctcttcca ggcgcgccag cttgcccgac cgtcccggca gggcgctgcc
     4261 gtcccccaca aattgggccg ggtgattgca aatctgcttg agcttcatga gcgtcgccag
     4321 caccagtcct ctgcgctcta taccctcgga gtcctcgatc ttctccatca tctctttcac
     4381 gaccgcctcg tagagtgtgg cctgctcgcg cgtcaagttg caatacacgc gcatctccat
     4441 cttatctggc aggtcggcga taatgctttt atcggttttc aagcggcgca gcaagaacgg
     4501 ctgaatgaca tttttgagcg tctccgcgcg ctccgcgtcg tgatagcgtt cgacaggaat
     4561 ggcaaactgc tggcggaaat cggtgggcga acccaggtag cccggattga ggaactccat
     4621 aatcgaccac agttcagaaa gccggttctc caccggagtg ccagtcagcg cgatcttatg
     4681 cctggccttg attgctttga tggcttgcga ctgcttggcc gcctcgttct tgatattctg
     4741 cgcctcgtct accaccacgt attcccactc gatggacgcg agatgctccc ggtcgcgcag
     4801 cgccagcgaa tacgtggtaa tcacgacatc atttcgcctc gcctccgcct caaaagcct
//
LOCUS       DASZAK010000575         1730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_190420, whole genome shotgun sequence.
ACCESSION   DASZAK010000575 DASZAK010000000
VERSION     DASZAK010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190420"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1645
                     /gene="recG"
                     /locus_tag="VGT44_10110"
     CDS             <1..1645
                     /gene="recG"
                     /locus_tag="VGT44_10110"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV2581192.1"
                     /translation="AMVPDHLPASIRSAGQLMPLPEAVSHIHYPENEAALKAARRRLG
                     FDELFLIQLGMQERRSRWQYEAHQGNAFKVDLAKVLIDAPNSAETARASGESEDSLGP
                     LAATLWSPVSINKPFEATLPFRFTGAQRRVIGEILDDLAQNRPMCRLLQGDVGAGKTA
                     VAAVTLFIAALNGYQSAIMAPTELLAEQHAQSISTMLEPFGIRTVLLTGSLKQRERTM
                     GRTAIENGEAMVAIGTHALIQEDVSFQRLGLVIIDEQHRFGVEQRDALRQKGYHPHML
                     VMTATPIPRTLALTLYGDLDVSVIDELPPGRQKVITRWRTGARRPEANTLIAHQVAEG
                     HQAFIICPLIEESETLAVKAATVEYERLSREVFPNLRLGLLHGSMKPAEKDATMRRFR
                     DHELDILVATSVIEVGIDIPNATVMVIEDAVRFGLSQLHQFRGRVGRGNHQAYCYVLS
                     ADASIQAQERLEVFQSTDDGFRLAEEDLRLRGPGDFIGVRQSGMPELHMADLSDTRLI
                     ELTRSLAAKLWQSDPYLRKPEHAALRERMHMFWQSVMAH"
BASE COUNT          375 a          504 c          479 g          372 t
ORIGIN      
        1 agccatggtt cccgatcact tgccggcatc gatacgttcc gcgggccagc ttatgcctct
       61 gcccgaagcg gtatcacata tacactaccc ggagaatgag gcggcattga aagccgcacg
      121 ccgccgcctt ggctttgacg aactcttttt gatccaactg ggcatgcagg aacggcgcag
      181 tcgctggcaa tatgaagcac accaggggaa tgctttcaaa gtcgacctgg caaaagtttt
      241 gatcgatgct cccaattcag cagagacggc gcgagcatca ggcgagagcg aagactcgct
      301 cggtcccctt gccgctaccc tttggtcacc ggttagcatt aataagccat ttgaggccac
      361 gctgccattt cgtttcaccg gagcccaacg gcgcgttatc ggcgaaattc tggacgatct
      421 cgcacagaac aggcccatgt gccgtctctt acagggcgat gtcggcgctg gtaaaactgc
      481 cgtggcagcc gtgaccctct ttatcgccgc cctcaacggc tatcagagcg ccattatggc
      541 ccctacagag ctcctggcag agcaacatgc ccagagtatc agcactatgc tggaaccttt
      601 cggtattcgc accgtactct taaccggcag cctgaaacaa cgcgagcgga cgatgggacg
      661 gacggctatc gagaatggag aggccatggt cgctataggc actcatgcac tcattcaaga
      721 agatgtgagt tttcagcgac ttggactggt tattatcgat gagcagcacc gttttggcgt
      781 agagcagcgc gatgccctgc gacaaaaagg ctaccatcca cacatgctgg tcatgactgc
      841 cacgcccatt ccgcgcaccc tggcgctcac actgtatgga gacctggatg tctcggttat
      901 tgacgagctt ccacccggtc gccagaaggt tattacgcgc tggcggacag gcgcccgtcg
      961 ccctgaagcc aataccctca tagctcacca ggttgcggaa ggtcaccagg cctttattat
     1021 ctgtccattg atcgaggaat ccgagacgct tgcagttaaa gcggctaccg tcgaatatga
     1081 gcgtttgagt cgcgaggtct tccccaattt gcgcctgggc ctgctgcatg ggtcgatgaa
     1141 gcccgccgag aaagacgcga ccatgcggcg tttccgcgat catgaactgg acatcctggt
     1201 ggcaacctcg gtcattgaag tcggtatcga tatacccaat gccacggtga tggtcatcga
     1261 ggatgcagtt cgttttggtc tctcacagct ccaccagttc cgcgggcgtg ttggccgagg
     1321 caatcatcaa gcgtactgct acgtgctcag cgccgatgcc agcatacagg cccaggaacg
     1381 ccttgaagta ttccagtcaa ccgacgatgg gtttcgcctg gcggaggaag acctgcgcct
     1441 gcgaggtcca ggtgacttta ttggcgtgcg ccagagcggt atgccagagc tgcacatggc
     1501 cgatctgagc gataccaggc tgatcgaatt aacccgttcc ctggccgcga aactctggca
     1561 aagcgaccct tacctgcgta agccagaaca tgctgctctg cgcgagcgca tgcacatgtt
     1621 ctggcagagc gttatggcac attgactgtt cacaccgcct cacgccgccc gagatgttgc
     1681 gggtcgtaac caatcatggt agcgtttgtc tggatttaga tgcttgcctg
//
LOCUS       DASZAK010000576         4597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_441645, whole genome shotgun sequence.
ACCESSION   DASZAK010000576 DASZAK010000000
VERSION     DASZAK010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441645"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VGT44_10115"
     CDS             complement(<1..651)
                     /locus_tag="VGT44_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581193.1"
                     /translation="MKLSRRAMIKDGMLVVSAGMVMPSIFSRGVASAMAQSLDGSHLA
                     QASTNNILIVVQMAGGNDGLNTVVPYTDPMYLKMRPTIGVQQSKVLALDNRLGLHPNL
                     APLKKLWDSGHLAIVEGVGYPNQSLSHFQAMDIWQTLDLTGNGSDGWLGKLVSGLVDQ
                     QGHPFKSLDIGVQTAQALQSISTQVPTVASVKSYAVAPDPADGDGGNARLQALLKLY"
     gene            complement(711..2360)
                     /locus_tag="VGT44_10120"
     CDS             complement(711..2360)
                     /locus_tag="VGT44_10120"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HEV2581194.1"
                     /translation="MDTNLFDDPTEPRPKNAAASGPTVPMTPGKFAATTPVANTGLAS
                     PSRRTFMGKSLSKVAIIGGASVAGVAAATVGGFALDQWVQKGGLTNLFHSSGPMASET
                     QIGHLLRKAGFGATQADLNTYRSLGFSGAVDQLLNYQQVSESDTENRLNALNLNLNSP
                     IDQQRWWLLRMAWTQRPLLEKMTLFWHGVLTSSFRKVGGKNAYVRMINQNNFLRAHAF
                     DTFDTILLGITADPAMLFYLDLTKSTKAHPNENYARELMELFTLGLGNYTQQDVYMGA
                     AALTGWHARGLTSQYYPNDHSNLTKTYLGHTGNLDYKDVVNILANHPATPWFISRKLF
                     TFFVYENPSVDDLKPLVASYTQSGHNMGAVMRTLLLSPQFSSSQAYRSRVKSPVEFTV
                     GAYRQLGLTDNGMSLPGIATLMGQTIFDPPNVAGWPGDKVSSLWLNSGTWMTRLNYVD
                     LLLVRGSNARNTTPAVNLQAIVDANKIDSPSAFVDYFASFLLDGNFEADHRTQLIDYF
                     TTSDTSRGGQKITLANGQSYPFNRVRGTLYLMMTLPEYQLN"
     gene            2545..4530
                     /locus_tag="VGT44_10125"
     CDS             2545..4530
                     /locus_tag="VGT44_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581195.1"
                     /translation="MKTITEQIDREAKADSATSLLPGDIKLAPRRLVWENHIPYFFLG
                     AMVVAILLMDAVLPFRDLWFHEATLTQLGDWSVWPSSLLFRNWALMPPVPFQHVNGTP
                     GILQSWIEFPILLGAFAVVFIAYFAALRRLPEYISWRIIYCSTILLGILYVLIPIVTS
                     PDIYSYIAYARMGLVYNLNPLTTLPTAINADAVYNYVLWVDQPSAYGPTWTIISSLVQ
                     WAVARLGLDGVLPMVLALRLLGLAAHLISVRLVWSISGKLQQLLGHSAPYPSRARVRA
                     TLAFAWNPLLLFEACVNAHNDAILLVFILLVIWILAQSKLAASLVGKESPRTVGFVST
                     RRWGWGGIQPWVSAIWGWQQTWGVRLLARIPTDLRTPIAASSMMALATCLKINIVLLY
                     PALLCYLWGQASEGRRLKCAAASIISFSGVIVCSYAPFWQGGAIFNVFSVNPSTYRAI
                     NTVASVLAYLYNSVAVWAGYPLGLSSGSPAERFLHTFSMGIFVMIYLVMLWRMVRASH
                     KMRTLNGLIYWMAVAWLLYCAIGSPWFWPWYLVTFFGLYALLEASSEVGEQDSQQDEK
                     KPQFPWSFPLFRTPWTARLLSFTMLSLYCFITWGPAHTFVPGLPGFLWGYLSGAWAWL
                     LPLVGVALLAWRRKGNRKDATISNWRQEGDRKGAPLQ"
BASE COUNT          900 a         1355 c         1267 g         1075 t
ORIGIN      
        1 gtacagcttc aacagcgcct gcaaacgcgc gttgccgcca tcgccatcgg cgggatcggg
       61 agcgacagca taggatttga cgctggcaac cgtcggcacc tgcgttgaga tcgattgcag
      121 cgcctgcgcg gtctgcacgc cgatatcgag cgacttgaag gggtggcctt gctggtcaac
      181 caggccggag accagcttgc ccagccagcc gtcactgccg ttgccggtca ggtcgagcgt
      241 ctgccagatg tccatagcct ggaaatgcga caggctctga ttggggtagc cgacgccttc
      301 gacgatagca aggtggccgc tatcccacag cttctttaag ggcgccaggt tggggtgcag
      361 acccaggcga ttatcgagcg ccagcacctt cgactgctgg acgccgatag tcgggcgcat
      421 cttcaggtac atggggtcgg tatacggcac gacggtgttg aggccgtcgt tgccacccgc
      481 catctgcacg acgatcagga tattgttggt ggacgcctgc gccaggtgcg agccgtccag
      541 cgactgcgcc atcgcggatg cgacgccgcg actgaatatg gagggcatga ccatgcctgc
      601 cgagaccacc agcattccgt cttttatcat cgctctgcgc gagagtttca tagcctttat
      661 ccttattttg aaccgggcga cctctaaggt cgccccaacg tttcaaagag ttaattcaat
      721 tgatattcgg gcaatgtcat cataaggtag agcgttccgc gcacacgatt gaacggatag
      781 ctctggccgt ttgccagggt gattttctgt cctccacgac tggtatcgct cgtcgtgaag
      841 tagtcgatta actgtgtgcg gtggtcggcc tcaaaattgc cgtccagcag gaacgaggca
      901 aagtaatcga cgaaggccga gggcgagtcg atcttattcg cgtcaacgat agcctgcaaa
      961 ttgaccgccg gtgttgtgtt gcgcgcattg gagccgcgca ccagcagcag atcgacgtag
     1021 ttcaggcgcg tcatccaggt cccgctgttc agccagagcg aactgacctt gtcgcccggc
     1081 cagcccgcca cattgggcgg atcgaaaatc gtctgcccca ttagcgtcgc gattcccggt
     1141 aagctcatgc cgttatccgt caagcccagc tggcgatacg ccccgaccgt gaactccaca
     1201 ggcgacttga cacggctgcg atacgcctgc gacgaagaaa actgcggcga gagcagcagg
     1261 gtgcgcatga ccgcgcccat gttatgcccg ctctgtgtgt aggatgcgac aagcggcttc
     1321 aagtcatcta ccgagggatt ttcgtagacg aagaaggtga acagcttacg cgagatgaac
     1381 cagggcgtgg ccggatggtt cgccaggatg ttgacgacat ccttgtagtc caggttgccg
     1441 gtgtgaccca ggtaggtttt cgtcagattg ctgtgatcat ttggataata ctgcgaggtt
     1501 aacccacgcg cgtgccaccc ggtcagcgcg gcagcgccca tgtagacatc ctgctgcgta
     1561 tagttgccca atcccagtgt gaacagctcc atcagctcgc gcgcatagtt ctcgttcgga
     1621 tgcgccttcg tactcttggt caggtcaagg taaaagagca tggccggatc ggctgtaatg
     1681 ccgagcagga tagtatcgaa ggtgtcaaag gcatgagcgc gcaggaagtt attctggtta
     1741 atcatgcgca cgtaggcatt cttgccgccg accttgcgga agctgctggt cagcactcca
     1801 tgccagaaca gcgtcatctt ctcaagcaag gggcgctgcg tccaggccat gcgcagcagc
     1861 caccagcgct gctgatcgat gggcgagttg agattgaggt tcagggcgtt aaggcgattc
     1921 tctgtatcgc tctccgagac ctgctggtaa ttcagcagct gatctaccgc gccgctaaac
     1981 cccaggctgc ggtaggtatt cagatcggcc tgggtcgcgc cgaaaccggc tttgcgcagc
     2041 agatgtccga tctgcgtctc gctggccatc gggccggaac tatgaaacag gttggtcaac
     2101 ccgccttttt gcacccactg atccagggca aatcccccaa ccgtcgcggc agccacgcct
     2161 gccacgctgg cccccccaat gattgctacc tttgaaagag acttgcccat gaatgtgcgc
     2221 cgcgacggcg acgccaggcc agtattggcg accggcgtgg tggcggcgaa cttgccgggt
     2281 gtcatcggaa ctgttggtcc agaagctgcg gcgttcttcg ggcgcggttc ggttggatcg
     2341 tcaaacaaat tggtatccat atatatttac tccactgaag agagctagcc agagcaacta
     2401 gaataagtga cctctgtcca tatcatttcg tcttagagct ttgtataccc taaggtatga
     2461 gttattcgct attgctagtg taacagcgaa acctcaagaa actctcaaga cgggttacat
     2521 attttctcaa gtagttcatc gcgcatgaaa acgataaccg aacagataga ccgcgaggcg
     2581 aaagcagact cagcgacctc tttgctacct ggtgatatca agcttgctcc gcgtcgcctt
     2641 gtgtgggaaa atcatatccc atatttcttc ctgggcgcaa tggtcgtggc cattttgctg
     2701 atggatgcag tcctaccctt tcgtgatttg tggtttcacg aagcaactct aactcagctt
     2761 ggcgattggt ctgtctggcc ctcgtcactg ctttttcgca attgggcatt gatgccgccc
     2821 gtcccttttc aacatgtcaa cggcacaccc ggtatcctac agagttggat tgaatttcca
     2881 atcttgctgg gtgcatttgc agttgtattc atcgcttact ttgccgccct gcgtcgcctt
     2941 ccagaatata tctcctggcg cattatttac tgctcaacga tcctgctcgg tattctttac
     3001 gtgctcattc ctatcgttac gtcgcctgat atctactctt acattgccta cgcgcgcatg
     3061 ggccttgtct ataacctcaa cccgctcaca acgctgccca cagccatcaa tgccgatgcg
     3121 gtctacaatt acgtactatg ggtcgatcaa ccatcagcgt atggcccgac ctggaccatc
     3181 atctcaagtc tcgtccagtg ggcggtagct cgcctcggac ttgatggcgt actgcccatg
     3241 gttcttgcgc tgcgcctgct cgggttggcg gcgcacctga tctcagtccg cctggtctgg
     3301 tctatttctg gcaagctcca acaattgctt ggacacagcg caccttatcc atcgcgcgcg
     3361 cgcgttcgcg caacactggc cttcgcctgg aatcctctcc tgctctttga agcctgcgtc
     3421 aatgcgcaca acgacgctat cttgctcgtc tttatcctgc tcgtcatctg gatactcgcg
     3481 caaagcaaac tcgctgcctc tctcgtaggc aaggagtcgc ctcgaactgt aggctttgtg
     3541 agcacccggc ggtggggatg gggtggcatc cagccctggg tatcagcaat ctggggatgg
     3601 caacaaacgt ggggagtgcg ccttctcgcg cgcattccaa ccgatctgcg tacacccatc
     3661 gctgcttcga gcatgatggc cctggccacc tgcctgaaaa tcaacatcgt cctcctctat
     3721 cccgctttgc tgtgctacct gtggggacag gcctccgaag gcaggcgctt gaagtgcgcg
     3781 gctgcctcaa tcatcagctt tagcggagtg attgtctgct cctatgcgcc cttctggcag
     3841 ggaggcgcaa ttttcaacgt cttcagcgtc aaccccagca cctaccgcgc cattaatacc
     3901 gttgcttcag ttctggcgta tctctataat agcgtagccg tctgggctgg ctacccgctt
     3961 ggtctgtcca gtggctcacc tgccgagcgc tttttgcaca cgttcagcat gggcattttc
     4021 gtcatgatct accttgtcat gctgtggcgc atggttcgcg ccagccacaa gatgcgcacg
     4081 ctcaacggcc tcatttactg gatggcggtc gcctggctgc tctactgcgc cattggttcg
     4141 ccctggttct ggccctggta cctggtcacc ttcttcggcc tgtatgcctt actcgaagcc
     4201 agcagtgagg taggtgagca ggacagtcag caagatgaga aaaaaccaca attcccctgg
     4261 tcgtttccgc tcttccgcac accctggaca gcacgcctgc tctcctttac catgctgagc
     4321 ctctattgct tcatcacctg gggtcctgcc cacaccttcg tacccggtct gcccggcttc
     4381 ctgtggggct atttgagcgg ggcctgggcg tggctgttac ccctggtcgg cgtggcactg
     4441 ctcgcctggc gtcggaaagg caaccgcaaa gatgcgacca tcagcaactg gcgtcaagag
     4501 ggcgaccgca agggtgcgcc cttacaatga cccgacgaat ggcgacgtgt agaatcactc
     4561 cgtagtcatt gtaagggcgc acccttgcgg tcgccct
//
LOCUS       DASZAK010000577         1957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_190448, whole genome shotgun sequence.
ACCESSION   DASZAK010000577 DASZAK010000000
VERSION     DASZAK010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190448"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..750)
                     /locus_tag="VGT44_10130"
     CDS             complement(124..750)
                     /locus_tag="VGT44_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581196.1"
                     /translation="MHVKLDILQDKGFVVLKDYDGPQVPPSEWESLEYMDWKSGGDTN
                     FAPIASALGEMECAGFWDHGKPDKGGIWTKNAAICPTLVEWTKRVGADYGRVRIIKLN
                     PNTEAEGVHNLHLDDNNRLNPDGEGWVVRAWLQLTDDPDSYMILREDKNDPSTESRIS
                     MPRNRQMLIDSQRMWHAAWHPGPNPRYALIVSFESGEALQRWIASQLP"
     gene            complement(837..>1957)
                     /gene="priA"
                     /locus_tag="VGT44_10135"
     CDS             complement(837..>1957)
                     /gene="priA"
                     /locus_tag="VGT44_10135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV2581197.1"
                     /translation="VLGSATPSVVSFYRAQRGDYRLVEMPNRVGGKLPPVEVIDLRSE
                     LHAGNTSIISRRLQDELEQVLGRRQQAILYLNRRGAASCVLCRECGFVAMCDRCDVPL
                     TYHSTERILLCHYCNAQSKPLRNCPQCASASIRYFGLGTEKVVDTIQRAFPTVRLLRW
                     DRDTAKNRRAHEQLLDRFANREADILVGTQMIAKGLDLPGVTLVGVISADIALMLPDF
                     SASERAFTLLTQVAGRAGRGEEAGQVIVQTFNPQHFCIDAASRHDYHEFYAAEIEARS
                     RYGYPPFRQFVKFTYSHENRRRSQNEALLLREKLDDWIARLGLPHTDIVGPAPALMER
                     LHGKYRWQMIARGPDLHPLLRVIDTPGWDIDIDPVSTM"
BASE COUNT          331 a          553 c          664 g          409 t
ORIGIN      
        1 aagcgctacc acctgggcgc aatgcattgc gtccctacat gccaaatgaa ccttgactat
       61 gtactacggg tttgtgacgc tttgcctgcc tggtcgcatg ccgatggttg atggaggtag
      121 cagttacggc agctggcttg cgatccagcg ttgcaaggct tcgccgctct cgaaggagac
      181 gatcaaggcg tagcgggggt ttgggcctgg gtgccaggcg gcgtgccaca tacgctgcga
      241 gtcgatgagc atctggcggt tgcgcggcat tgaaatacgc gactcggtgg aggggtcgtt
      301 tttatcttcg cgcaggatca tataggagtc ggggtcgtcg gtcagttgca gccaggcgcg
      361 cacgacccag ccttcgccgt cgggattcag gcggttgttg tcgtcgaggt gcaggttatg
      421 cacgccttcc gcctcggtgt tggggttcag tttgatgatg cgcacacgcc catagtcggc
      481 tccgacgcgc tttgtccatt ccaccagagt tgggcagata gcggcgtttt tggtccagat
      541 gccgcccttg tccggcttgc cgtgatccca gaaaccggcg cattccatct cgcccagggc
      601 tgaggctatc ggggcgaaat tggtgtcgcc gccgctcttc cagtccatat attccaggct
      661 ctcccactct gacggtggga cctgcgggcc gtcgtaatct ttcaggacga caaatccctt
      721 atcctgcaaa atatccagtt tgacgtgcat gtgtacttac tcctcaagtt gcatacaagt
      781 ttcttacgat tggttgagta cggggtaccc aacccattgt atcataacct ttgcgctcac
      841 atggtgctga ccgggtcgat atcgatgtcc cagccggggg tgtcgatgac gcgcagcagg
      901 ggatggaggt cggggccgcg cgcgatcatt tgccagcgat atttgccgtg caggcgttcc
      961 atcagggcgg gggcgggacc gacgatgtcg gtgtgtggca ggcccaggcg cgcgatccag
     1021 tcgtcgagct tctcgcgcag cagcagggct tcgttttgcg agcggcggcg attttcgtgg
     1081 ctgtaggtga acttgacgaa ctggcggaag ggtggatagc cgtagcggct gcgcgcctcg
     1141 atttcggcgg cgtagaattc gtggtagtcg tgacgcgagg cggcgtcgat gcagaagtgc
     1201 tgcgggttga aggtctggac gatgacctgc cccgcctctt cgccgcgacc cgcgcggccc
     1261 gcgacctggg tcagcagcgt aaaggcgcgt tccgaggcgg agaagtcggg cagcatcagg
     1321 gcgatatcgg cggagatgac gcccaccagt gtgacgccgg gcaggtcaag ccccttggcg
     1381 atcatctgcg ttcccaccag gatatccgct tcgcgattgg cgaagcgatc cagcagttgc
     1441 tcgtgggcgc ggcggttctt cgcggtgtcc ctgtcccagc gcagcagacg cacggtaggg
     1501 aaggcgcgct ggatggtatc gacgaccttt tccgtcccca gcccgaagta gcgtatgctc
     1561 gcgctggcgc actgcgggca attgcgcagg ggtttgcttt gagcattgca atagtggcag
     1621 agcaggatac gctcggtgga gtgataggtg agcggcacgt cgcagcggtc gcacatggcg
     1681 acgaagccgc attcccggca cagcacgcag ctggccgcgc cgcgccggtt gaggtagagg
     1741 atggcctgct ggcgcctgcc caacacctgc tcaagctcgt cctgcaagcg gcggctgata
     1801 atgctggtgt tgccggcgtg gagttcgctg cgcaggtcga tgacttccac cgggggcagc
     1861 ttgccgccga cacggttggg catctctacc aggcgatagt cgccgcgctg cgcgcgatag
     1921 aaggatacga cggacggggt ggcgctgccc agcacga
//
LOCUS       DASZAK010000578         3356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_263818, whole genome shotgun sequence.
ACCESSION   DASZAK010000578 DASZAK010000000
VERSION     DASZAK010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263818"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1322
                     /locus_tag="VGT44_10140"
     CDS             <1..1322
                     /locus_tag="VGT44_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation-efflux pump"
                     /protein_id="HEV2581198.1"
                     /translation="LLTYFAVRVADRPPDATHNYGHAKVENLSAFFEAGLLMLTALWI
                     IYEATRRLIFHEGHVDASIWAFLVMLVAIAVDFTRSRALMRVARRLGSQALEADALHF
                     KTDIWSSAVVIGGLLVVRLTQSITLPAWLGQADAIAALGVSAIVILVSLRLVRETIDA
                     LLDRAPGALTQDIEAAIKQVDGVSEVRRVRARRAGNKLFADVVIAAPRTYTFEQTHSL
                     TERIERAALLASRHSSPQLEVDVLAHVEPAAAPSETVAEQIYYLAELQGVHAHDIHVR
                     EVSGQLEADFDLEVQANMDLQEAHNEATRLEQAIRQSNPLVQRVTTHLEAPDAEIVRR
                     QDVTQSYPDMAKRILSLADEVAGKGSAHDVHIYRPSTGPLNLDNGTCENQEELDLVLH
                     TIFAPHIPLSQVHIQAEEIKRTLRSAYPCLGSVVIHEEPPEAEEIQR"
     gene            1362..3152
                     /locus_tag="VGT44_10145"
     CDS             1362..3152
                     /locus_tag="VGT44_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581199.1"
                     /translation="MLLNRQLRLGRLTISLATLIAAIIIVADAALRLMLIVNNYPTTN
                     SDEATVGLMALHINQGHDFPAFLYGQASLGALEAYVGAALFFILGPSVFALRLGVLLF
                     FIAFLICMYLLTRLLYSKGLALVTLVLLSIGTPEILYRSIPAFAGHAETPLFGALIIL
                     ISAQLGLASQVYDPEAGLRPSRRLAVLYLLWGIVAGVAFWNDALAGAFIVMGAFFILV
                     CCRRTLKPSTLTALFAGLVIGILPVILNDLLVQGGHKSLEVLGFILGGASLANHSIFE
                     HLAAAFLVSLPVATGGTAICAITNWSTDSWPVTSQSRASVIQCTALHGIWATVMLAAW
                     IAAVVIEVSILSRRWLLARAKQRAKPDTSDGSSTDSAPLQSPSLLEQRAVVLRYARLM
                     LLGSAALTWLVFAMSQIAIDYPWGDHRYLIGLGVATPAVIWPLWLAITDRNSKPTLLS
                     RAQLTKVLSMVILLAFAGALVIGTADTFEQMVSSTKATQQQQELADTLVRLHLRHIYS
                     EYWTCDLVSFLTKEQVICSVLDEHLNPGVNRYLPYASMVAQDPNAAYIFPISSPQAQA
                     FAARAAQSKQPYNVLQFDNYILYQPVTKGL"
BASE COUNT          670 a         1041 c          936 g          709 t
ORIGIN      
        1 ccctgctgac ctacttcgcc gtgcgcgtcg ccgaccgacc gcccgacgcg acgcacaact
       61 atggccatgc caaagtcgaa aatctttcgg ccttcttcga ggctggcctg ctgatgctta
      121 ccgcattgtg gatcatctat gaggcaacgc ggcggctcat cttccacgag gggcatgtcg
      181 atgccagcat ctgggccttc ctcgtcatgc tcgtggctat cgccgtcgat ttcactcgct
      241 cgcgcgccct gatgcgcgtc gctcgccggc tgggcagcca ggcgctagag gccgacgcgc
      301 tgcacttcaa aaccgacatc tggagctccg ccgtcgtgat cggcgggctg ctcgtcgtgc
      361 gactcaccca gagcatcacg ttgcccgcct ggttgggcca ggccgatgcc atcgccgcgc
      421 tgggcgtttc cgccatcgtg atcctggtca gcttgcggct ggttagagag accatcgacg
      481 cactgctcga ccgtgcgccg ggcgcgctga cccaggacat cgaagcagct atcaagcagg
      541 tggatggcgt cagcgaggtg cgccgggtac gcgcccgacg cgcgggcaac aagctcttcg
      601 ccgacgtggt tatcgcagct ccgcgcacct acacctttga gcagacgcac tccctgaccg
      661 agcgcatcga acgcgccgcc ttgctggcct cgcgtcattc ctctccgcag ctcgaagtcg
      721 atgtactggc gcatgtcgaa ccggcagccg cgccgagcga aacggtcgcc gagcagatct
      781 attacctcgc ggaactacaa ggtgttcacg cgcacgatat tcacgtgcga gaggtgagcg
      841 ggcagctcga agcagatttt gacctggaag tgcaagcgaa catggattta caagaggcgc
      901 ataatgaggc aactcgcctg gaacaggcca tacggcagag caatccactc gtacaacggg
      961 tcacgaccca ccttgaagcg cccgatgctg aaatcgtgcg ccgccaggat gtgacgcaga
     1021 gctaccccga tatggcaaag cgcattctct cgctggccga tgaggttgcg ggcaaaggga
     1081 gcgcgcacga cgtgcatatc tatcgcccat ccaccggccc actcaacctg gacaatggta
     1141 cgtgcgaaaa ccaggaagag ttagacctgg ttctccatac catcttcgcg ccgcacatcc
     1201 ctctcagcca ggttcacatc caggccgagg agatcaagcg caccctgcgc agcgcctatc
     1261 cctgcctcgg ctcggtcgtc atccacgaag aaccaccaga agccgaagaa atccaacgct
     1321 gaacaagaac aaagatgaga tgaagggacg tggttttcga tatgcttttg aatagacagt
     1381 tacgcctggg aaggctgacc atcagcctgg ccacgctgat tgccgctatc atcattgtgg
     1441 cagacgctgc gctgcgcttg atgctcattg tgaataatta ccccaccacc aatagcgacg
     1501 aggccaccgt tggcttgatg gcattgcata ttaatcaagg ccacgatttc ccagcgtttt
     1561 tatatgggca ggcgtcgttg ggcgcgctgg aagcctacgt tggcgcggcg ctgttcttta
     1621 ttttgggccc gtcagtattt gcgctgcggc tgggcgtact gctcttcttc atcgcctttc
     1681 tcatctgcat gtacttgctc actcgcctgc tctacagcaa agggctggcg ctcgtgacac
     1741 ttgtgctgct cagcatcggc accccggaga ttttgtatcg ctccattccg gccttcgccg
     1801 gacatgccga aacgccgctt ttcggcgcgc tcattatcct gatcagcgcg caactggggc
     1861 tggcttcaca agtctacgac ccggaagcgg ggctgcgtcc ttcccggcga ctggcggtgc
     1921 tctatctcct ctggggcatc gtcgcgggag ttgcgttctg gaatgatgcc ctggcagggg
     1981 cattcatcgt catgggcgcg tttttcatcc tggtctgttg ccgccgaacg ctcaagccat
     2041 ccacccttac tgcccttttc gcaggtctcg taattggtat tttgccagtg atccttaatg
     2101 acctgctcgt gcaaggaggt cacaagagtc tggaagtcct cggatttatc ctgggtgggg
     2161 caagccttgc caatcattcc atcttcgaac acctggcagc agcctttcta gtcagcctgc
     2221 cggttgcaac gggtggaacc gctatttgcg ctatcacgaa ctggagcacc gactcctggc
     2281 cggtcacgtc gcaatccagg gcctccgtca ttcaatgcac cgcgctgcat ggcatatggg
     2341 cgacagtgat gctggcagcc tggattgcgg cggtcgtcat agaggtgagc atcctcagcc
     2401 ggagatggct gctcgccagg gcaaagcaac gcgctaaacc tgatacgagt gatggatcgt
     2461 ctactgactc agccccgctc cagtcgccct ccctacttga acagcgcgct gttgtgttgc
     2521 gctatgcgcg cctgatgctg cttggtagcg ccgcactcac ctggctcgtc ttcgccatga
     2581 gtcaaatcgc catagactat ccctggggcg atcatcgcta cttgattggt ctgggagtcg
     2641 ctacaccggc ggtgatctgg cctttgtggc tggcaatcac cgacaggaac agcaaaccga
     2701 cgctgctctc ccgcgcacag ctcacgaaag ttttgagcat ggttattctg ctggcctttg
     2761 caggagcgct ggtcataggc acggcggaca cgtttgagca gatggtgtcc agcaccaagg
     2821 ccactcaaca gcagcaagag ttagccgata cgcttgttcg cctgcacctg cgccacatct
     2881 acagcgaata ctggacatgc gacctcgtca gcttcctgac aaaggagcag gtcatttgca
     2941 gcgttctgga tgaacatctg aacccaggcg tcaatcgcta tcttccctat gcgtctatgg
     3001 tggctcagga cccgaacgca gcctacatct ttcccatctc gtcgccccag gcgcaggcgt
     3061 ttgccgcgcg agcagcacaa tcgaagcagc cgtataacgt cttgcagttc gacaattaca
     3121 tcctgtacca gcctgtcaca aagggcttgt aaaagcagcc tgcgcgaagt gactcgccaa
     3181 cagggacatg tccctgttgg cgagtcacag ctacacaaaa tgcccgatct gtgcatcgtt
     3241 ggtcaaccag agcgtaccgt cagcgccaga aacgataccc cgcaggttgc ccagatcaaa
     3301 cgagctgcct ccgttgtcga agtatgtccc gggcgggaac gggtaaaact tgaaga
//
LOCUS       DASZAK010000579         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_227584, whole genome shotgun sequence.
ACCESSION   DASZAK010000579 DASZAK010000000
VERSION     DASZAK010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227584"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1277)
                     /locus_tag="VGT44_10150"
     CDS             complement(<1..1277)
                     /locus_tag="VGT44_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2581200.1"
                     /translation="MESSTTRTDVAQADSEEQVTAIPAAGRDLALVSAAHAVIHAIAV
                     LMPLIYPLIQVEYHLSYTQIGLIVAVPNAVGGLIQVVFGLLSRFILRKVMLGVGNIFV
                     GISTFFTGTIVGFWSLMTWSMLAKLAGAPQHPVGSSYLTDRYGRRRHGYALAWHVAGG
                     NIGTLAVPLIAGPLLGVWGWRPVLYLAALPGILIGIAILILVDEHGLLDKRDVRRASS
                     SDVPSTGSDDRLPQARERISTRLYKTLVEPLRNRTLAIIMLVSIIAAGGRGLGNVTTY
                     VPLYLQNVLHLNAGLISVFFTLLLLGSVLGPLLGGRLSDRLGRRRLLFFFYGASSLAL
                     LLFLVLAAPGRPLWLVPLSLVLMGFVIYAESPLLQAYLADTASPAMRDAAFGLYFSLA
                     FGIGSIWSAVLGWMIDHFGFGSAFWTMALSFLVA"
     gene            1454..>1636
                     /locus_tag="VGT44_10155"
     CDS             1454..>1636
                     /locus_tag="VGT44_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HEV2581201.1"
                     /translation="MLEQKIPETAPVQGTLFDTTELERLVHDRQRWEDTTLRQSLKRL
                     PERDNLITTSSVPVNRL"
BASE COUNT          426 a          514 c          451 g          245 t
ORIGIN      
        1 gcgaccagga acgacagcgc catcgtccag aaggccgagc cgaagccgaa atggtcgatc
       61 atccagccca gaaccgcgct ccagatcgag ccgatgccga aggccagtga aaagtacagg
      121 ccaaaagcgg cgtcacgcat ggcaggactg gcagtatccg ccaggtatgc ctggagcagt
      181 ggactttcag cgtagatgac gaagcccatc aggacgagcg agagcggcac gagccagagc
      241 ggtcggccag gcgcggccag cacaaggaaa agcagcagcg ccaggctgga cgcgccatag
      301 aagaagaaca gcagcctgcg ccgccccagg cggtccgaga gtcgcccacc cagcagcggc
      361 ccaagcacac tgccgagcaa cagcagcgta aagaaaacgc tgataagccc ggcattgaga
      421 tgcaggacat tttgcaggta aagcggcacg taggtagtaa cgttgcccag gccgcgcccg
      481 cccgccgcaa tgatcgagac cagcatgatg atcgccagcg tccggttgcg cagcggctcg
      541 acaagcgtct tatagagccg tgtcgaaatg cgttcgcgcg cttgcggcag gcgatcatca
      601 ctccccgtgg acggaacgtc tgaggatgat gccctgcgaa cgtcgcgttt atccagcaaa
      661 ccgtgttcat ccaccaggat caaaatggca atgccgatca agatgccggg cagcgcagcc
      721 aggtacagga caggacgcca gccccacacg ccaagcagcg gtccggcgat gagcgggacg
      781 gccagcgtac cgatgttgcc cccggccacg tgccacgcca gggcatagcc atgccgccgg
      841 cgcccgtagc ggtcggtcag atacgaactg ccgacgggat gctgcggcgc gcccgccagt
      901 ttcgcgagca tactccacgt catcaacgac cagaatccca caatcgtccc ggtgaagaat
      961 gtgctgattc ctacaaaaat attgccgacg cccagcatca ccttgcgcag gataaagcgg
     1021 ctgagcaggc caaaaacgac ctgaatcagc cccccaaccg cattaggcac ggcaacaatc
     1081 agcccgattt gcgtatagct aagatggtat tctacctgga taagcgggta aatcaacggc
     1141 atcaaaaccg cgatagcatg aataacagcg tgagccgcgc tgaccagcgc caggtcgcgc
     1201 ccggcagcag gaatagccgt gacctgctcc tcgctgtctg cctgagcaac atccgtgcgc
     1261 gtcgtcgatg attccatgaa actcccttct aatcaagtct aacatagccc ttaactaccg
     1321 tcgagggtaa gagtgaccat gaatccgggg aaatcttacc gattttgagc agccctcaaa
     1381 cctgaatatg atacaatacc catacagtaa caccattcca gtaaaagccc tcaacgagga
     1441 ggaatcatca atcatgctcg aacaaaaaat accggaaacc gcccccgtac aggggacgtt
     1501 gtttgataca accgaactcg aacgcctggt gcatgaccgc caacgctggg aagataccac
     1561 cttgcgccag tcgctgaagc gcctgcccga acgcgacaac ctcatcacta cttccagcgt
     1621 gccggtcaac cgcctc
//
LOCUS       DASZAK010000580         3109 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_654506, whole genome shotgun sequence.
ACCESSION   DASZAK010000580 DASZAK010000000
VERSION     DASZAK010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654506"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..765
                     /locus_tag="VGT44_10160"
     CDS             316..765
                     /locus_tag="VGT44_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581202.1"
                     /translation="MTKPLRDTLYQQVQVHRYQCLKCKRTFRVYPEGTNRAQSSQRVK
                     GLAVMLYLLGLSYGATSLALEARGVYLCKSRVYDAVQETASRVPGLKREQVFAGVKTP
                     ALGGDLTSVKCKGEWLPLGITVDPISGLALTIDALPAQDRKRASRLD"
     gene            800..991
                     /locus_tag="VGT44_10165"
     CDS             800..991
                     /locus_tag="VGT44_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581203.1"
                     /translation="MTDDADGFKTVADAVGVQHQVWKAHVLRNTETLIERYQPLVAKD
                     ADGSLKAIGVSRSRRQQPT"
     gene            1052..1381
                     /locus_tag="VGT44_10170"
     CDS             1052..1381
                     /locus_tag="VGT44_10170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581204.1"
                     /translation="MHRRYLEAAPPQEGEHQSLAYRLRLLFLDRWNLWHRLTRYRKWK
                     GPKGETLDGTTNACERAIGWWIKERYRSMRGYKVPENAVRVSRFLAWCGNFLNAEDGA
                     ILVGLLH"
     gene            complement(1726..1908)
                     /locus_tag="VGT44_10175"
     CDS             complement(1726..1908)
                     /locus_tag="VGT44_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2581205.1"
                     /translation="MLQGAGAPPHHHTGSSELFYVVSGAAQMLADESVMTADEGDLSG
                     LIRCSDHARLNANGAN"
     gene            2093..2452
                     /locus_tag="VGT44_10180"
     CDS             2093..2452
                     /locus_tag="VGT44_10180"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2581206.1"
                     /translation="MSYNQTVETSKIQAIPAPFPNTKGTATLKILNSDQSLGPALVLL
                     RMEPGSEIARHLQEQTTETLYVLDGDFINEGISYPVGTELNVKPMIAHGPHTTKNGCS
                     VLVMFSYPSVLDDFKWA"
     gene            2672..3016
                     /locus_tag="VGT44_10185"
     CDS             2672..3016
                     /locus_tag="VGT44_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581207.1"
                     /translation="MLFGKGAELFLAHVITQPPDFDQVLSVQVGTHQFTDEDLLQGVR
                     VVFPGRVNTISNKIKEGEQLLGKVQVVGQHAPGIVDLTVAVGIELYVETDELADPNTM
                     SMESSGLRQVRE"
BASE COUNT          735 a          796 c          937 g          641 t
ORIGIN      
        1 tgtagcatag tggtctcgtg ataggcgatg gcgtgctcct tgcagaaggc tttgacgatg
       61 ggctgggctt ttcttaattt gttcctgggc atgattggga acaggtgatc agggtctcac
      121 agtaaaagga agttctgtga ctgttcagaa agcggtaaaa ctggggtaaa atagaaggga
      181 aagagaaccc aaaggaggca gacgcatgcg tttacggatc ctgttacgcc atccgtgaac
      241 cctgaagcaa tcactgtgcc cacccgatgt gcctatgctg gctgtggagg gaggaaggtg
      301 tacctccgcc aggaagtgac gaaacctttg cgcgatacgc tgtatcagca ggtccaggtg
      361 catcgctatc agtgtttgaa gtgcaaacgc acctttcggg tctatcccga gggaaccaac
      421 cgcgcacaga gctcgcaacg ggtgaaaggg ctagcggtga tgctgtatct gctggggtta
      481 agttatgggg cgacctcctt agcgttagaa gcccgtgggg tctacctctg taagagtcgg
      541 gtctatgatg cggtgcagga gacggctagc cgcgtacctg ggctcaagcg agagcaggtg
      601 tttgcagggg tgaaaacacc cgccttagga ggcgatctga ccagcgtcaa atgcaagggc
      661 gagtggttac ccttaggcat caccgttgat cccatcagcg gcctcgcctt aacgattgat
      721 gcgctcccag cgcaagatag gaagcgcgct tcaagactgg attgagccaa tcgccaagag
      781 cgtggaagcg acggtcttgg tgaccgatga tgctgatggg ttcaagacgg tggctgatgc
      841 ggtgggcgtg cagcaccagg tctggaaagc acatgtgctg cgcaataccg agaccttgat
      901 cgaacgctac cagccgttgg tggccaaaga tgctgatggc tcactcaaag ccattggtgt
      961 gagtcgaagc cgcaggcagc agccgactta acgcgagtag cgcgaactgg tcaagagccg
     1021 acagcgggag caggctcctg aactggaagc gatgcaccgg cgctatctgg aagccgcgcc
     1081 gccgcaagaa ggggagcacc aaagtctggc ctatcgccta cgactcttgt ttcttgatcg
     1141 ctggaatctc tggcatcgct tgacgcgcta tcgcaaatgg aaaggaccca aaggggaaac
     1201 gctggatggc accaccaatg cctgtgagcg tgccatcggc tggtggatca aagagcgcta
     1261 tcgcagcatg cgtggctaca aggtgcccga gaatgcggtg cgtgtcagtc gcttcctggc
     1321 ctggtgtggc aacttcctca acgcggagga cggagccatt ctggtagggc ttttgcacta
     1381 agtaagagct tccagggatc ctcacggctt tttcttatct gccctctcgg taccaactat
     1441 cgaacgatca cacggtgacg taccgctcaa ggatgccggg tctcaagtgg ggaagaacct
     1501 tgtggcttgc ctcgttggcg ggggggccat tcgagtacac cggcagagag ctgcgcgatg
     1561 cacatttcga cctgaagatc tcacccgccg tgttcgacga agtcgcggcg gaacttgcca
     1621 acacccttga tgacttcaac gtgcccgagc gcgagaaggg agaggtcctg gccgccttcg
     1681 ctggagagaa gggcgaagtt tcggctggtt ctcagcctgg ggctgtcaat tggcgccgtt
     1741 ggcgttgagc cgggcgtgat ccgaacagcg aatgagaccg gagagatcac cctcgtctgc
     1801 ggtcatgacg ctctcgtcgg cgagcatctg ggccgcgccc gacacgacgt agaacagctc
     1861 actggagccg gtgtggtggt gcggcggtgc cccggcgccc tgcagcaaag ggatccgctg
     1921 atttggagag acagctactc cgatgaaagt gagagtgttt gttgctctcc aacacaggcc
     1981 ttttggccca tgacttctcg tgcatttcgc cctacacgag aagtaggtca agcacacctg
     2041 ttttggctga gacgaagaaa gaaaaaaaga gacagaaaga aggtacagtt ctatgagtta
     2101 taaccaaacg gtcgaaacct cgaagatcca ggctataccg gcaccatttc ccaacaccaa
     2161 agggactgcc acgctcaaga ttttaaacag tgatcagtcc ttgggtccag cactggtcct
     2221 actgagaatg gagccgggga gcgagattgc aagacacctg caagagcaaa caacggaaac
     2281 gctgtacgtc cttgatggtg atttcatcaa cgagggcatc tcctatccgg tgggcacgga
     2341 actcaatgtc aagccgatga ttgctcacgg tccacatacg acgaagaatg gctgctccgt
     2401 cctggtcatg ttcagctatc cgtctgttct cgatgatttc aaatgggcct agccgtttga
     2461 ggacgaaaac tggtccaaac gggatatgac agagaaccct ggtgaaaacg gcatctttcc
     2521 gagcaagaga tggacgacct caccaggagg tcaatttggg gttagatggc tctcccgaac
     2581 tctctcatca aaagttcggg agagccctca tagccgttca ttcagtcaag agcttcatcc
     2641 ccgggggccg gggcgacacg atctcgaata cttgctcttt ggcaaggggg cagagctgtt
     2701 tctggctcac gtgatcacgc aaccccctga ttttgatcag gtgctctcgg tgcaggttgg
     2761 cacccatcaa tttacggatg aagacctgct ccaaggagtg cgggtggtct ttcccggacg
     2821 ggtgaatacc atctcaaaca aaatcaagga aggagagcag cttttgggca aggtccaggt
     2881 agtcggacag catgctcctg ggatcgtaga cctaacggtt gcagtgggca tcgagctcta
     2941 cgttgaaaca gacgagcttg cagatccaaa caccatgtcg atggagagtt caggtttgag
     3001 gcaagtacga gagtaacagg cccataaatg taccaatcat catccgcgat ggcgccgctg
     3061 cattcagcag tgtcggcacc agcacaacaa ggggtaccaa agtcctggc
//
LOCUS       DASZAK010000581         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_333799, whole genome shotgun sequence.
ACCESSION   DASZAK010000581 DASZAK010000000
VERSION     DASZAK010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333799"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VGT44_10190"
     CDS             <1..376
                     /locus_tag="VGT44_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEV2581208.1"
                     /translation="EQSLYERIGGVNAISMVVDLFSDEVVKNPKLNVNPALKDWNQSG
                     KLPGLKFMRTLWICQAAGGPFQYTGKDMHEAHKDLHITSAEFDEVGVEIARALDHFNV
                     PEREKQEVLAAIVARKTEVVNG"
     gene            584..886
                     /locus_tag="VGT44_10195"
     CDS             584..886
                     /locus_tag="VGT44_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV2581209.1"
                     /translation="MTSRSPAESMIGIAIGPSGLGWVKSDLPVQVLVIMGLAFLLFLA
                     SLDVELGRLRGRLLGLARLGFVLSLGLAISVGYALDAAGQVRSPLFVGIVLSATTL"
     gene            complement(1057..1552)
                     /locus_tag="VGT44_10200"
                     /pseudo
     CDS             complement(1057..1552)
                     /locus_tag="VGT44_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917362.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(1528..2136)
                     /locus_tag="VGT44_10205"
     CDS             complement(1528..2136)
                     /locus_tag="VGT44_10205"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEV2581210.1"
                     /translation="MAARVATMLLLTLRGTPTWYYGDELGMQDVPIPPDLAHDPQGKR
                     QPGYGRDSVRTPMQWDAGPNASFCPANVDPWLPLAVDYRQINVAEEREDAHSQLSLTR
                     ALLTLRRKKPALSGGSYRALEDGSEHCLVYLREQGKQRLVVALNFSAEAQTVRLPEFG
                     RGRILISTALDREEQISLAVLALRGHEGCIVELADGSSTRPM"
BASE COUNT          465 a          606 c          624 g          447 t
ORIGIN      
        1 agagcagagt ctttacgaac ggatcggggg cgtcaacgcc atcagcatgg tcgttgacct
       61 cttcagcgac gaagtcgtca agaatccgaa actcaatgtg aacccggccc tgaaggactg
      121 gaaccaatcg ggcaagctgc caggtctgaa attcatgcga acgctctgga tctgccaggc
      181 ggccggcgga ccctttcagt acaccggcaa ggacatgcac gaagcgcata aagatctcca
      241 catcacgtcc gcagagttcg acgaggttgg agtagagatc gccagagctc tggaccactt
      301 caacgtgccg gagcgcgaaa agcaggaagt cttggccgcc atagtcgcaa ggaagaccga
      361 ggtggtcaac ggctaaaacc ggagactcgc gttgttgcag ccatgttgca cggaacgcgg
      421 ccgcatctca aggtagaggt gctcgactct cagcgggaac ctatgcggag atcgacccag
      481 gcgtcatagc aattgagtgg cggccttcgc cggcgtcgag tgccatcggg tacgctcggg
      541 aggcacctgg aatgccacga caccttcgtg tcggcggaag gccgtgacgt cgaggagccc
      601 ggcggagtcc atgatcggga tcgccatcgg cccgtcgggc ttggggtggg tcaaatccga
      661 ccttcccgtc caggtcctgg tgatcatggg gttggcgttc ctgctgttct tggccagcct
      721 ggacgtcgaa ctcgggcggc tacggggtcg gctgctcgga ctggccagac tggggttcgt
      781 actgtcgctc ggcctcgcca tatcggtagg ctacgctctc gatgcagcgg ggcaggttcg
      841 ctctcccctt tttgtcggga tcgtgctttc ggccacgacc ctttgaccat ctgatcatat
      901 tgctggcgga tcagttccca gttgatggca cgcgagagaa tcaggcgaag tttgggatac
      961 tgctcggcat ctgctgcatt caccaaatag agcttctgta catcgatgtt cttgaggctc
     1021 gaaaagtaaa cttcatgcgg aaacccctaa tggcatctat ctgatgagta gatagcaaag
     1081 caaaagaacc ggtagagcca acattagcgc cctttttaga acagatatgc taagcagccg
     1141 gcacaccctt ctgatcatgt tcatggcttc atatccctgc aaagtctgcc atgtggtctc
     1201 tgacgaaaag aaacccatcc ccagcttgac gagctgtttg gtgaagcgat gatcttgctc
     1261 gacaacattg ttgagatatt tactttgacg cagttcacaa ggatctggca tcgtcccctc
     1321 cgcttgcagc tccttgaagg cctttggata ggccgcattt ttatcaacgg tgatgacgcg
     1381 gggagtgctg atatggggtg cagcaagcgc tttcgtgaag aagcatctgg ccgagtgacg
     1441 catctctagt gggctttaac agaaattcca gggtgtttcc ctgcgaatct acagtacgat
     1501 agaggtacat ccacaccttc ttcactttca cataggtctc gttgacgagc catcagccag
     1561 ttcaacgata cacccctcat ggcctcgcag agcaagcaca gccaggctta tctgctcctc
     1621 ccgatcaaga gcggtcgaaa tgaggatgcg gcctctgcca aattcgggca acctcacagt
     1681 ttgtgcctct gctgaaaagt tcagcgcaac caccaggcgc tgcttcccct gttctcgcag
     1741 ataaaccaaa cagtgctcgg agccgtcttc gagggcccga tagctcccac cggagagggc
     1801 cggtttcttg cggcggagag tcagcagggc ccgagtgagc gaaagctgcg agtgcgcatc
     1861 ctcgcgctcc tcagccacat tgatctgccg gtaatcgacg gccagaggca gccacggatc
     1921 gacgttggcc ggacagaagc tcgcgttggg tcctgcgtcc cactgcatcg gcgtgcgcac
     1981 ggaatcgcgt ccatagccag gctggcgctt gccctgtggg tcatgggcca ggtcgggtgg
     2041 gatagggacg tcctgcatgc caagctcatc accataatac caggtaggag ttccccgcag
     2101 cgtcagaagg agcatcgtag cgacgcgagc agccatacgc cc
//
LOCUS       DASZAK010000582         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_689865, whole genome shotgun sequence.
ACCESSION   DASZAK010000582 DASZAK010000000
VERSION     DASZAK010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689865"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGT44_10210"
     CDS             complement(<1..281)
                     /locus_tag="VGT44_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581211.1"
                     /translation="MDGTIITSSPSNSINFPNSITFKITASDSSSNIVSADLVVDLQN
                     NNGPETHVVAFGAPQHTIRPVFQENTTGNNFVPPGATITYYWRFDDAAGT"
     gene            complement(728..979)
                     /locus_tag="VGT44_10215"
     CDS             complement(728..979)
                     /locus_tag="VGT44_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581212.1"
                     /translation="MAGHPVAFLAAVAGVNSVPAAEASAITMMKALPAETTSPGALVA
                     LEMAVALEALEKIAAVEENEQTGYTGSAGHRIWVRRSRC"
     gene            991..3654
                     /locus_tag="VGT44_10220"
     CDS             991..3654
                     /locus_tag="VGT44_10220"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEV2581213.1"
                     /translation="MLLSNAMQTMRHWSGKFAAARAGNVDASPPLVLYRPGVPQPPQP
                     PKPAQKVRRTSWKRSHVLRVSMQMRRRRERWQRSGPNGKKALRISLTIVAVVLVALVS
                     SGVASGYAYYQSQYPLVQGVANNQIEQSTHIYDRNGNLIYTAYDGKYGRSTPITYNEV
                     PGVMQDAMIAAEDHTFWDNVGVDPQGILRAASEYASSGSVQSGGSTITQQVIKNLTHD
                     TQVTIQRKLSEGTLAIGLTQQYPKWKILEMYFNVSPFGAQELGVEAAVEDYFGLHAQC
                     DANFKCIPGIAFLDRDLTKCKNPKDLNTCQSNPLLELARASLLAGMPQNPPGYDPTVN
                     QSTKAAALARQDYVLNEMMGLNMGINLGLGDQTKDSGLGPITTDVIQKVEALTQKMNF
                     VGFKYTKNDPHFVDWIIPQIENALGNGDPQLGAHLFLTGGFNIRTTIDSNLENFIEND
                     VRHHLDDPEYQIFLGDYGPLNTVHNVNDSAVVVEEAKTGEILAMDGSADYNNNSDPRV
                     AGQVNVATSLRSPGSAFKPIVYATAFQMGWYPGIVLPDKETYFYTGSQSQDVNAVVNG
                     VDATYHPYDYGQTWHNLNSNIVLDIANSFNVPAVKTLEFAGTENVLQMARRFGITDID
                     QDAVNYSNAYDAQYHSKKRFTAEQILGPSFALGAYDVSLLQMVSAYQVFADQGKRIPP
                     QYVLDIWDNYGHHIYQYNPSNPPAVQVISPQIAYLMSSILTNNAARALEFAPDMVLTT
                     SDWDNRPVAAKTGTSEGFQDNLTLGYTPDIVVGTWSGNANGDLMKHTVGISGAAPIWH
                     DALEYASGKCFTKGIFPGGWTSCNDMSFPADGFTQPAGVLQQYVNTVNGLMGNGYDTY
                     MLTGDVPVQTGFTSTNGTTTPTGSPTASPTP"
BASE COUNT          917 a         1040 c         1038 g          808 t
ORIGIN      
        1 gtcccggcgg catcatcgaa acgccagtag tacgtgatag tagcgccggg agggacaaag
       61 ttattaccgg ttgtgttttc ttgaaagact ggtctgatcg tatgttgagg tgcgccaaat
      121 gcaacgacgt gtgtttcggg accgttattg ttttgtagat cgaccaccag gtctgccgat
      181 acaatattac tactcgaatc gctggcagtg atcttaaagg tgatcgagtt gggaaaattg
      241 atcgagttgg atggagaaga agtgataatg gtgccgtcca atgcaagcgc cagggcgtga
      301 tgtatactgg ttagtggggt aagagatatc gacagtacag ataaaagagc gatgagacaa
      361 caggcggata ctgcaatgcg gccataatga cgaaaagaca tcaactggtt cctctccaat
      421 acaagcctgg caattgacga acaggcttac gtagattgag ctggcaggat atggtataga
      481 aaactttcgt tcctattgta cctgattcca ggcaaagtgt acacagatgt atcccccaaa
      541 atggtagatg cgttaaagcg gcttctcctg ttcgttgcct tagcatagat tggtattttc
      601 gctgaaaccc gcaagggaca cgttctatga tagagacgat ttgactgcgc tgcttttgcg
      661 tactattaag gaaatagaat gaataacgat aacaatccat attggcaagg ggagacccac
      721 taccctccta acaacgggaa cggcgtaccc agatccggtg gcctgctgag ccagtatagc
      781 ccgtctgctc attctcctca actgccgcaa tcttctcaag cgcctcaagc gccacagcca
      841 tttccagagc aaccagggcc ccggggctgg tcgtctccgc cggtagcgcc ttcatcatcg
      901 ttatcgccga tgcctcagca gcagggacac tatttacccc agcaacagca gccaggaatg
      961 ccacagggtg gcccgccatg gcaggcgccc atgttactct ctaatgcgat gcagacgatg
     1021 cggcactggt cgggcaaatt cgcggctgca cgcgccggta atgtggatgc gagtccgccg
     1081 ttagtattgt atcgcccagg cgtaccgcag ccgccgcagc cgccgaaacc agcgcagaaa
     1141 gtgcgacgca catcctggaa acgatcgcat gtcctgcgtg tctcgatgca aatgcgccgc
     1201 cgccgggaac gctggcagcg atctggaccc aatgggaaga aagctctgag gatcagcctt
     1261 acaattgttg cggttgtgct ggtggcgctg gtctcctcgg gcgtcgcttc gggctatgcg
     1321 tattatcaga gccagtatcc actcgtacaa ggagtggcga ataaccagat tgagcaatcg
     1381 acgcacatct atgatcgcaa tggcaacctg atctatactg cttacgatgg caaatatgga
     1441 cgcagcacgc caatcactta taacgaggtt cccggcgtga tgcaggatgc catgatcgcc
     1501 gccgaagacc ataccttctg ggataacgtg ggcgtcgatc cccagggtat cctgcgcgct
     1561 gccagcgagt atgcctcttc gggcagtgtg cagagcggcg gcagcacaat cacccagcag
     1621 gtcattaaga atctgacgca cgatacccag gtgacgattc agcgcaaact gagcgaaggc
     1681 acgctcgcca tcggcctgac ccagcagtat cccaagtgga aaattctgga aatgtacttt
     1741 aatgtttcgc cctttggagc gcaggaactt ggcgtggagg ctgccgtcga agattacttt
     1801 ggcctgcacg cccagtgcga tgccaacttc aaatgcattc caggcatcgc gtttctggac
     1861 cgcgatctca ccaaatgcaa gaatccgaaa gatctcaaca cctgccaatc caatccattg
     1921 ctggagctgg cgcgcgcttc gctgctggca ggcatgccac agaacccgcc cggctacgat
     1981 cccaccgtca accaatcgac caaagcggct gccttggccc gccaggatta tgtattaaat
     2041 gagatgatgg ggctgaacat gggcatcaac ctgggcctgg gcgaccagac gaaggattct
     2101 ggcctggggc caatcacgac tgatgtaatc cagaaggttg aggcgttgac gcagaagatg
     2161 aacttcgtcg gctttaaata taccaagaac gacccccatt ttgtggattg gatcatcccg
     2221 cagatcgaga acgcactggg caacggcgac ccccaacttg gcgcgcatct cttcctgacg
     2281 ggcggcttta atattcgcac gaccatcgac tcgaacctgg aaaacttcat cgaaaatgat
     2341 gtgcgccacc acctggacga tcctgagtac cagattttcc tcggcgacta tgggccgctg
     2401 aataccgttc acaacgtcaa tgactcggcg gtggttgtgg aggaggccaa aaccggcgag
     2461 attctggcga tggatggcag cgcggactac aacaataaca gcgacccgcg ggtcgccgga
     2521 caggtcaacg tggccacgtc gctgcgctcg cctggttccg cgtttaagcc gattgtctac
     2581 gcgacggcct tccagatggg ctggtatccc ggcattgtct tgcccgataa ggagacgtat
     2641 ttctacaccg gttcgcagtc ccaggatgtg aacgctgtag tcaacggggt ggatgcgaca
     2701 tatcatccgt atgactatgg ccagacatgg cataacctga actcgaacat agtgctggat
     2761 attgccaact cgttcaatgt cccggcggtc aagacgctgg agttcgccgg taccgagaat
     2821 gtgttgcaga tggcgcgcag gtttgggatt accgacattg accaggacgc ggtaaactac
     2881 agcaatgcct acgatgcgca atatcacagc aaaaagcgtt tcactgccga gcaaattcta
     2941 ggaccatctt tcgcgctggg tgcgtatgat gtctcgctgc tgcaaatggt cagcgcctac
     3001 caggttttcg ccgaccaggg caagcgcatt cctccgcagt acgtgctgga tatctgggat
     3061 aactacggac accacatcta tcaatataat cccagcaacc cgccagccgt gcaggtcatc
     3121 agcccgcaaa tcgcctacct gatgtcctcg atcctgacga ataacgcggc gcgcgccctg
     3181 gaatttgcgc ccgacatggt gctgacgacc agcgactggg acaatcgccc ggtagcggcc
     3241 aagaccggca cgtctgaagg gttccaggat aacctgacgc ttggctacac gccggatatc
     3301 gtggttggca cgtggtcggg caacgccaac ggagatctga tgaagcatac cgttggtatc
     3361 agcggcgctg ctcccatctg gcacgatgcc cttgaatatg ccagcggcaa atgcttcacc
     3421 aaagggatct tccccggcgg ttggacgagc tgtaacgata tgagcttccc ggcagatgga
     3481 ttcacgcaac ccgccggggt gctacagcaa tatgtcaata ccgtcaatgg cctgatgggg
     3541 aacggctacg atacgtacat gctcaccggt gatgtaccgg tgcagactgg ctttacctca
     3601 acgaatggga cgactactcc cactggctcg ccaacggcct cgcctacacc atgatgcggt
     3661 gttatggcct ggatgtgatg ggtgaataag cccgccacat ccaggaaaaa tcctcttgac
     3721 aatcacccct tgcgagtgct attctgtatt tgcactctca aggtgagagt gctagcaatc
     3781 accattgaca ggcatcgtat cat
//
LOCUS       DASZAK010000583         6243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_120585, whole genome shotgun sequence.
ACCESSION   DASZAK010000583 DASZAK010000000
VERSION     DASZAK010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6243
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120585"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VGT44_10225"
     CDS             <1..895
                     /locus_tag="VGT44_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2581214.1"
                     /translation="GKAIFRRPAMPLHNNIFHVPYGDADAVEQQLRIAREVGNDIAAV
                     IMEPVQGEAGAIVPPDDFWPRLRQICDEYEVLLIADEVQTGMGRTGRLWGVDHWEVEP
                     DIIASAKALGGGVMPIGAFMSTPKIWSVMNSNPFIHTSTTGGNPLACAAAIAAINVTL
                     EEHIPEQAAEKGEYFIQQLRDIASRHQDVYTNITGKGLLIGQHFVNDDIGYAVASGLF
                     KRGVLISGTLNNSRVVRIEPPVVITHEEIDTVLNRLEDTLLELHGSVTLPAMPDEWDE
                     QSGAAGVASAILVNGTDTAVA"
     gene            910..2073
                     /locus_tag="VGT44_10230"
     CDS             910..2073
                     /locus_tag="VGT44_10230"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HEV2581215.1"
                     /translation="MHIIVLGGAGAMGRVTVRALTEYADIDHITIADYREGRARELAT
                     SLNSSKVEPVQIDVNDEARLRSLLRGADVALSAVDYVYNLPILKACIAEKTHYADLGG
                     LFHMTRTLMALHDEAEAAGITAILGMGGTPGITNLLARVAADQLDRVESIKVELGCSD
                     STPSMAPLVAPYSIRTILDEFTKEPQVFQDGAWHPQQPLSGQEEMLFPPPVGRATAVF
                     SLHSECATFPISFRDKGINYVSFKIAFPSDFITKLKFLVDLGFGSNEPLDIHGSKVSP
                     REVLARLLERFPTGEVEPQDCDVLRVVTTGQASGDAATIIEQVRVLPYRRWNVSAGAL
                     DTGVPLAIAGHMLAGGEITRRGAHGPELCVPVELFFRELSRYNMYVEEIQEWP"
     gene            2089..2601
                     /locus_tag="VGT44_10235"
     CDS             2089..2601
                     /locus_tag="VGT44_10235"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2581216.1"
                     /translation="MDEQATTKTQLLDWIKSARAPLDRLIAPLSEEQMLQPGVENKSS
                     VKDLLAHISTWEDHLARRLAAAARDRVADVYVIDPAEPADPGGIDTLNEFIFTRNAQL
                     PLQQVLSNFHRSFHEVLQAVEALSDYDLFDSQGLAQLFGYSAERIIGSDTFYHYPEHI
                     QSISAWMNGT"
     gene            2776..4272
                     /locus_tag="VGT44_10240"
     CDS             2776..4272
                     /locus_tag="VGT44_10240"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HEV2581217.1"
                     /translation="MADYKLLINGELVDSVSGKKVEDIGPASGEPIAAVPVTTTEDVE
                     RAVTAARQAFDDGRWSKLGHGIRAAMLEKLAVAIEAHASELAELESQDAGKPIKLARD
                     GDIPFSIDNLQFFAGAARHLSGIAASDYTGGHTSIIRREPVGVVASLAPWNYPFMMAA
                     WKIGPALAAGNTVVLKPSIETPLTTLKLGEIALEAGLPPGVLNIVTSGPGVAQALSSH
                     KQVNMVSITGSTESGKHVMRAGADTIKHVHLELGGKAPFIVFADANIEAAANGAVAGG
                     YVNCGQDCTAATRIYVQDSIYDQFLASFLAKVRSIRVGNPASESTDMGPLISASQRGT
                     VEGFVERALRDGANIVTGGRRAHVAGLEKGYFFEPTVIAQDHNDAEIIQSEVFGPVVV
                     VSRFSTEEEVLQKANDVVYGLASSVWTSDVFKAMRAASSLQFGTVWVNDHLPLTSEMP
                     HGGYKESGIGKDLSMYAFEDYTQIKHVMIELTNDDRKGWHYTIFGDAE"
     gene            4562..5698
                     /locus_tag="VGT44_10245"
     CDS             4562..5698
                     /locus_tag="VGT44_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HEV2581218.1"
                     /translation="MEERELRDLETIFAQVEDPRIERTKRHRLRDIIIIAICGMICGA
                     DGWVGIEEFGKAKEAWLAERLDLPNGIPSHDTFGRVFALIDPKQFEESFRQWVQGISP
                     TIKGVVAIDGKTSRRTYDQQADKKALHLVSAWATENRLVLAQVATEEKSNEITAIPVL
                     LRQLALAGCIVTIDAMGTQTKIAEQIIEQEGDDALALKDNQGNLYDEVLATFALAEKE
                     AFATVECESDRTVEKAHGRLEIREYWTISDPAILAYLNAEKRWKGLKGIGMVRAERRI
                     DQEITKETRYFLLSFSSVKTFAYAVRSHWGIENCVHWVLDLAFREDESRVRLGHADEN
                     LAVLRHISLNLLRQEPSSRVGIHTKRLKAGWDNTYLPRVLAGVN"
     gene            5940..>6243
                     /locus_tag="VGT44_10250"
     CDS             5940..>6243
                     /locus_tag="VGT44_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581219.1"
                     /translation="MDHLKGLWRHRFARLGMLVTLASMLIGMVAISPSSANVAQAWEN
                     GSCHLNSANGNIKHVIYVQFDNTHFSRDNPNVPSDLEQMPNLLNFITNNGTLLDNHH"
BASE COUNT         1385 a         1831 c         1761 g         1266 t
ORIGIN      
        1 tggtaaggcc atcttccgcc gcccggctat gccgttgcac aataacatat tccatgtccc
       61 ctatggtgac gccgacgccg tcgagcaaca gctgcgcatc gcgcgcgagg tcggcaacga
      121 tatcgccgcc gtcatcatgg agccggtgca gggcgaggcc ggcgcaatcg tgccgcccga
      181 cgacttctgg ccgcgcctgc gccagatttg cgacgaatac gaagtcctgc tgattgccga
      241 cgaagttcag accggcatgg gccgcacggg caggctgtgg ggtgttgacc attgggaggt
      301 cgagcctgat atcatcgcct ccgcgaaggc gctgggcggc ggcgtcatgc ccatcggcgc
      361 gttcatgtcc acgcccaaaa tctggagcgt catgaacagc aacccgttca tccatacttc
      421 caccaccggc ggcaacccgc tggcctgcgc cgccgccatc gccgccatca acgtcaccct
      481 ggaagagcat atcccggagc aggccgccga gaagggcgag tacttcatcc agcagctgcg
      541 cgacatcgcc tcgcgccacc aggatgtcta taccaacatc accggcaagg gcctgctcat
      601 cggccagcat ttcgtcaacg acgatatcgg ttatgccgtc gcgtcgggcc tcttcaagcg
      661 cggtgtgctg atttccggca cgctcaacaa ctcgcgtgtc gtgcgtattg agccgcccgt
      721 cgttatcact cacgaggaga tcgatacggt cctcaaccgc ctcgaagata ccctgcttga
      781 actgcatggc agcgtcaccc tccccgccat gcccgacgaa tgggacgagc aaagcggtgc
      841 ggcaggcgtg gccagcgcca ttcttgtgaa cggcacagat accgccgtcg cctgaaaagg
      901 aaccttctta tgcacatcat tgtcctgggc ggcgcgggcg cgatggggcg cgtgactgtg
      961 cgcgccctga ctgaatacgc tgatatcgac cacattacca tcgccgatta tcgcgagggg
     1021 cgcgcgcgcg agctggcgac ttctctcaat agcagcaagg tcgagccggt gcaaatcgat
     1081 gtcaatgacg aggcgcggct gcgtagcttg ctgcgcggcg cggatgtggc gttgagcgcg
     1141 gtggattacg tgtataacct gcccattctc aaggcgtgta tcgccgagaa aacgcattat
     1201 gccgatctcg gcggtctctt ccacatgacg cgcacgttga tggcgttgca cgacgaagcc
     1261 gaagcggcgg gcattaccgc tattctcggc atgggcggta cgcccggcat caccaacctg
     1321 cttgcccgcg tcgccgccga ccagcttgac cgcgtagaga gcatcaaggt cgaactgggt
     1381 tgcagcgact ccacgccctc gatggctccc ctcgtcgcgc cttattccat tcgcaccatc
     1441 ctcgacgaat tcaccaaaga gccgcaggtc ttccaggacg gcgcgtggca tccgcagcag
     1501 cccctgagcg ggcaggaaga gatgctcttt cccccgcccg ttggccgcgc taccgccgta
     1561 ttctccctgc actcggaatg cgctaccttc ccgatctcct tccgcgacaa gggcatcaac
     1621 tacgtttcgt tcaagatcgc cttcccatcc gatttcataa cgaagctcaa gttcctggta
     1681 gacctgggtt ttggcagcaa cgaaccgctt gacatccacg gcagcaaggt ttccccgcgt
     1741 gaagtgctgg cgcgcctgct cgaaagattc ccgaccgggg aggtcgagcc gcaggactgc
     1801 gacgtgctgc gcgtcgtcac aaccggccag gccagcggcg atgccgctac catcatcgaa
     1861 caggtaagag tgttgcccta tcgccgctgg aatgtcagcg cgggcgcgct cgataccggc
     1921 gtcccactcg ccattgcagg tcatatgctc gccggtggcg agatcacccg tcgcggcgca
     1981 cacggcccgg aactctgcgt ccccgtagaa cttttctttc gcgaactgtc caggtacaat
     2041 atgtatgtcg aagagataca agaatggccg taaggtaagg agtacatcat ggacgaacaa
     2101 gccacaacaa aaacgcagct cctcgattgg atcaagagtg cgcgcgcgcc gcttgaccgg
     2161 ctgatcgcgc cattgagcga agagcaaatg ctccaacccg gtgttgaaaa caagagttca
     2221 gtcaaagacc tgctcgccca tatctctacg tgggaagacc atctcgctcg gcggctcgcg
     2281 gcagccgcgc gcgatagagt agccgatgtc tacgtcattg accccgccga gccagcggac
     2341 cccggcggga tagacaccct caacgaattc atcttcactc gaaacgcgca gttaccattg
     2401 cagcaggtac tgtcaaactt ccatcgttcc tttcatgaag tcttacaagc cgtcgaagcg
     2461 ctctccgact atgacctgtt cgattcccag ggactcgcgc aactcttcgg ctattcagca
     2521 gagcgtatca tcggcagcga tacgttctat cattatccag aacatatcca gtcgatcagc
     2581 gcgtggatga acggtacgta atcgcgcttt gctgtgatac aatgatagaa tgcaagcgca
     2641 ggtcattcga caaaatcgta gatgatttgc tgaaaaacca cgataatcgt acactaattg
     2701 gcaattgagc gtgttttcga gctgaatggg gcaatgtagc cccgctattt cgcacatccg
     2761 aaaggagcaa cagaggtggc cgattacaaa ttattgatta acggcgaact cgtcgatagt
     2821 gtttcgggca aaaaggtaga agatattggc ccggccagcg gcgagccgat tgccgcggta
     2881 cccgtcacga cgacggaaga tgtcgagcgc gcggtgactg cggcgcgcca ggcgttcgat
     2941 gacggcagat ggtcgaagct ggggcatgga atacgcgctg ctatgctgga gaagctggcg
     3001 gtggctatcg aagcacacgc ctccgaactg gccgagctgg agtcgcagga cgccggaaaa
     3061 ccaatcaagc tggcgcgcga tggcgatatc ccgttctcga ttgataacct gcaattcttc
     3121 gcgggcgcgg ctcgccacct ctccggcatc gcagccagcg actacaccgg cggccatacc
     3181 agcatcatcc gtcgcgagcc ggtcggcgtc gtggcgtccc tggccccctg gaactatccc
     3241 tttatgatgg cggcctggaa gatcggcccc gccctggcgg ctggcaacac cgtcgtcctc
     3301 aagccctcaa tcgaaacgcc gctcaccacg ctcaagctgg gcgagattgc cctggaagcc
     3361 gggttgcctc ccggcgtttt gaacatcgtc accagcggcc ccggcgtagc gcaggcgctc
     3421 tccagccata agcaggtcaa tatggtctcc atcaccggca gtacggagag cggcaagcat
     3481 gttatgcgcg cgggcgcgga taccatcaag cacgtgcatc tcgaactcgg cggtaaagcg
     3541 cccttcatcg tcttcgcgga tgccaacatc gaggccgccg cgaatggcgc ggtcgccggt
     3601 ggctacgtca actgcggcca ggactgcacc gccgcgacac gcatctacgt gcaggacagc
     3661 atttacgatc aattcctggc ctccttcctg gccaaagtca ggtcgattcg cgtcggcaac
     3721 cccgctagcg aaagcaccga catggggccg ttgatctccg ccagtcagcg cggcacggtc
     3781 gagggtttcg tcgagcgcgc cctgcgcgat ggcgcgaaca tagtcacagg cggcaggcgc
     3841 gcccacgtgg cgggcctcga aaaaggctac ttcttcgaac cgaccgtcat cgctcaggac
     3901 cataacgacg ccgaaatcat acagagtgag gtctttggcc ccgtcgttgt tgtctcccgc
     3961 tttagcactg aagaggaagt cctccagaag gccaatgatg tcgtctacgg cctggcctcc
     4021 tccgtctgga caagcgacgt gttcaaggcc atgcgcgccg ccagttcgct gcaattcggc
     4081 accgtctggg tcaacgacca cctgccgctc acctccgaga tgccccacgg cggctacaaa
     4141 gagagcggca tcggcaagga cctgtcgatg tatgcttttg aggactacac gcagatcaag
     4201 catgtcatga tcgagctgac caatgacgac cgcaaaggct ggcactacac catcttcggc
     4261 gacgcggaat agctttactg ggagttgccc ccccttgtca tgctgagcgc agcgaagcat
     4321 ctggcctaat gctccgagat gcttcgctgc gctcagcatg acaggtttac ctgtacgaac
     4381 cacgcgacgc tactggctca tttcttaacc ctcacttaat ccgccttaac ttccccacaa
     4441 agctctcatg ctacaatttt catggcatat atgcctgaat tcgtcaattg atcagggcaa
     4501 tcgcatctaa atttggagga ggtgggtgat agaataggag ctaaaaacgg gagaagatgg
     4561 gatggaagaa agagaactac gagacctgga aacgattttt gctcaagtag aagatccgcg
     4621 tatcgagcga acgaagcggc atcgattgcg cgacatcatc atcatcgcga tttgcggaat
     4681 gatttgtgga gcagacggat gggtagggat tgaagagttt gggaaagcaa aggaagcctg
     4741 gttggcagaa cggctagatt tgcccaacgg gataccttct cacgatacct ttgggcgagt
     4801 ctttgccctc attgatccca aacaatttga ggagagtttt cgtcaatggg tccaggggat
     4861 cagcccgacg atcaaaggag tcgtggcgat cgatgggaag acgagtcggc gcacgtatga
     4921 ccagcaagca gacaagaagg cgcttcatct ggtaagtgcc tgggctacag aaaaccgcct
     4981 ggtgttggct caagtcgcga ccgaggagaa atcgaacgag atcacggcta ttccggttct
     5041 cttgcgacag ctcgcgctgg cgggctgtat tgtcacgatc gatgcgatgg ggacgcaaac
     5101 caaaattgcc gaacagatta ttgagcaaga aggcgatgac gccctggccc tcaaggacaa
     5161 tcaaggaaat ctctatgatg aggtactggc aacctttgct ctggcggaga aagaggcatt
     5221 cgcgacggtt gagtgtgagt cagatcgaac cgttgaaaaa gcgcatgggc gtttggaaat
     5281 cagagaatat tggacgatta gtgatccggc gattttggcc tatctcaatg cagagaagcg
     5341 atggaaaggc ttgaagggga ttggaatggt tcgagcggaa cggcgcattg atcaagagat
     5401 cacgaaggaa acccgctact ttctgctgag cttctcttcg gtgaaaacgt ttgcttacgc
     5461 agtgcgaagt cactggggca ttgaaaactg tgtgcattgg gttctggatc ttgcctttcg
     5521 tgaagacgaa tcacgggtga ggctcggaca tgcagacgaa aatctcgcgg tgttacgtca
     5581 catcagcctc aatttgttac gccaagaacc atcgtctcgg gtcggcatcc acaccaaacg
     5641 tctcaaggct ggctgggaca acacctatct cccgcgcgtt ctggctgggg tgaattagat
     5701 gcgattgccc tggtcaattg atgcgctcgt ttgacgattt atttgttttg tgcattactg
     5761 acggtagtat tgcatgtcct caaagattgg cgccaaccat cgcttgagga gtggcttcag
     5821 cggggggctg aacaataccg tcgtttcttg ccaggttatg atcctcttac ccggttttgt
     5881 ttgtcgtctt ttagcgcggc atcttagctt cacaaacggt tactaacaga ggaggaacta
     5941 tggaccactt gaaaggactc tggcggcatc gtttcgcgcg cctcggtatg ctggtgacgc
     6001 tcgcatccat gctgataggc atggtggcga tttcaccatc atccgcgaac gttgcccagg
     6061 catgggaaaa tggcagctgc catctcaact ccgccaatgg caacatcaag catgtcatct
     6121 acgtccaatt cgacaacacc cacttctccc gcgacaatcc caatgtccct tccgatctcg
     6181 aacagatgcc aaacctgctc aactttatca ccaataacgg gacgctgctc gacaatcacc
     6241 aca
//
LOCUS       DASZAK010000584         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_227828, whole genome shotgun sequence.
ACCESSION   DASZAK010000584 DASZAK010000000
VERSION     DASZAK010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227828"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..1665
                     /locus_tag="VGT44_10255"
     CDS             283..1665
                     /locus_tag="VGT44_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581220.1"
                     /translation="MMRLRYHRPLWFFLACSLVVVLAACGSPSSPGGGSGNTGGAIPG
                     ATQISSQPGAQPTTPKPPSQPPTTACPAAGSGRAAVMNALALGRGQALVYVYKDSAAT
                     WHLRRYSVGSGQKTDIYTTAAGQIEDAQVSANGQWVIFLLDFYPAMRTDASAKIQMIR
                     VDGQDLQTLYCFPTNENYSRLISGNVGTGTLPVTLQLSPNQQALLFSVDTGEQQSTVY
                     DQDMTSGQTQVVYQVAADANYTTSVVTWLDNNNAYLITQGRSQPAPPSTVRLLNIPAA
                     LSSANNATTTVLQGGGRMREFSLDTSFDGAKLYSGNCLLAGSPFQTTIAVEPAHGGTA
                     QTIYQPPASICIDAIRVISPTTLLMLVTDDNTSTLVIQHEVWTMNVNGTAQHGLTTLA
                     PNDSRFQMNPNSQYVWSNVSRDGSLYALQQETNQNIENLVYGPVNGGNVTTLANGSSN
                     TLILVGWTTM"
     gene            complement(1676..2095)
                     /locus_tag="VGT44_10260"
     CDS             complement(1676..2095)
                     /locus_tag="VGT44_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEV2581221.1"
                     /translation="MAERSNWNSKIIDEFHATEGKPGGYWAGKPLLLLTTTGAKSGQR
                     HTTPTMYLPDGERLIVFASKGGAPTNPDWYTNLVAHPEVTVEVGNETYEGIATVLNGD
                     ERDRLYAKHAGLYPAFAEYQAKTTRKIPVIALSRREG"
     gene            complement(2168..>2381)
                     /locus_tag="VGT44_10265"
     CDS             complement(2168..>2381)
                     /locus_tag="VGT44_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581222.1"
                     /translation="FQNAPPRRVGDRFIGFLHGHGSASFHIFSISGYVEMLARIWGIV
                     KMARAIWSLLWLSLTIYLTVTEKVIE"
BASE COUNT          530 a          700 c          644 g          507 t
ORIGIN      
        1 catcgtaggg acgcgattca tcgcgtcctg gtggctccac tcaatcaagg gcaagcacat
       61 aatgacacat gacaacataa ggcggccact atagcaggga agacgagggc gaccgcaagg
      121 gtttagttat cgcgccattg atgaggaaca ttcgatcata gtaaagttgc gtaactcact
      181 ctgctcaggt gaacatcttc atcgataagc tcaccagttg ccgtttacac tgatgatgag
      241 attttgttga ttgtagacaa aaaccaatga aggaaataga aaatgatgcg tttacgttat
      301 catcgaccgt tatggttctt cctcgcgtgt tcgcttgttg tggtgctggc ggcatgtggt
      361 tcgccgtcgt cgccgggcgg cggttctggc aataccggcg gcgctattcc cggcgcaacg
      421 caaatatcat ctcagcctgg cgcgcagcca acaacgccaa agccgccctc gcagccgcct
      481 actactgcct gtcccgcggc gggcagcggg cgcgcggcgg taatgaatgc cctggcgctc
      541 ggtcgcggtc aggcgcttgt ttacgtctac aaggacagcg ccgctacctg gcatctgcgc
      601 cgttatagcg tgggctctgg ccagaaaacc gacatctata ctactgccgc aggacagata
      661 gaggacgcgc aggtctccgc gaatgggcaa tgggtgatct tcctgctcga tttctacccg
      721 gccatgcgca cggatgccag cgcgaaaatc cagatgatcc gcgtagatgg ccaggatttg
      781 cagacgctct actgcttccc taccaatgag aactactccc gtttaatcag tggaaatgta
      841 ggcaccggaa cgctgccggt tacgctgcaa ctatcgccca atcaacaggc gctgcttttc
      901 tcggtcgata ccggcgaaca gcagagtacg gtgtacgatc aggatatgac cagtgggcaa
      961 acgcaagtag tgtaccaggt cgctgctgac gccaactaca ctaccagcgt cgtgacctgg
     1021 ctggacaaca acaatgccta ccttatcacc cagggccgct cgcagcccgc gccgccatcc
     1081 accgtccgcc tgctgaacat ccccgcggcg ttgagcagtg cgaacaatgc cacgacgacg
     1141 gtcttgcaag gcggcgggcg aatgcgcgaa ttctcgctgg acaccagctt cgatggcgcg
     1201 aagctctaca gcggcaactg cctgctggcc ggctcgccgt tccagaccac catcgccgtc
     1261 gaaccggcgc acggcgggac agcgcagacc atctaccagc ctcccgcttc gatctgtatc
     1321 gatgcgatcc gcgtcatttc gcccacaacc ctgctcatgc tcgtcactga tgacaacacg
     1381 agtacgctcg tgatccagca cgaggtctgg acgatgaacg tcaacggcac agcccagcat
     1441 ggcctgacga cgctggctcc caacgacagc aggttccaga tgaacccgaa ctcacaatac
     1501 gtctggtcga acgtctcccg cgacggcagc ctgtacgcac tgcagcagga aaccaaccag
     1561 aatatcgaga acctggtcta cggcccggtc aatggcggaa acgtcaccac gctcgcgaac
     1621 ggttcgagca ataccctgat cctggtcggc tggacgacga tgtagcgcca gaaagttacc
     1681 cttctctacg gctcagtgcg attacgggaa tcttgcgggt cgtcttcgcc tggtattcgg
     1741 cgaacgcggg atacaatcct gcatgtttcg catagagccg atcccgctcg tcaccgttga
     1801 ggacggtagc gatgccctcg taggtctcgt taccaacttc gacggtgact tccgggtgag
     1861 cgaccaggtt agtgtaccaa tcgggattgg tcggcgcgcc gcctttggat gcgaaaacaa
     1921 tcagacgttc gccatcgggc aggtacatcg ttggcgtggt gtggcgttgg ccgctcttcg
     1981 cgccggtggt cgtgagtaat agcaaaggtt tgcctgccca gtaaccaccg ggcttgccct
     2041 ccgtcgcgtg aaattcgtca attatcttgc tgttccagtt gcttcgttca gccatagttc
     2101 ctcctgatta tgcttgtgcc ggttgcccgg cagggctttt gcgtgcgatg tatgtgcgga
     2161 tatgactcta ttctatcact ttctctgtta ctgtcaaata tatagttaat gacagccaga
     2221 gcaatgacca gatcgccctg gccatcttga caattcccca aattcgtgct aacatttcaa
     2281 catatcctga aatactgaaa atatggaaag aagctgaccc gtgaccatgc aagaagccta
     2341 taaagcgatc gccgacccga cgaggcggcg cattttgaaa t
//
LOCUS       DASZAK010000585         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_86148, whole genome shotgun sequence.
ACCESSION   DASZAK010000585 DASZAK010000000
VERSION     DASZAK010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86148"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="VGT44_10270"
     CDS             complement(<1..853)
                     /locus_tag="VGT44_10270"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2581223.1"
                     /translation="MFVHSELAAPTKKLSLPTLTLTSTYALGVNLVWLAYNIFILPVQ
                     IQSVTSEATKGIAVGVLAGVAIGIAVIVNIIAGIISDHSNSRFGRRRPIMFWGMMLTL
                     PFILMPVFLPLSLFTVSVAYLGMQIFTNVASGAWQPTLADFVPTEQRGLSAGIKGVFT
                     LIGSAIGVGLITALFAANLRNAAFMVIAGFFLVSTVVNVVAIWKLDKPLPDMKTLKMK
                     EVLVDMFRVQKRAGISFFWWFVLASFMIYMGVSGFQFFGVYYLEGVLHITAAADVAHA
                     IQVSGLIN"
     gene            complement(973..1299)
                     /locus_tag="VGT44_10275"
     CDS             complement(973..1299)
                     /locus_tag="VGT44_10275"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEV2581224.1"
                     /translation="MVTILPYLERILDRFSDPSLQETFKNFTKTLQFEFPDTQQRFLL
                     TIVNGVATLEEKAIPDPDMKTIANTDVLAGIMDKKINPMTAFMTRKIKAQGAQEDLLK
                     LQKLML"
     gene            complement(1342..2361)
                     /locus_tag="VGT44_10280"
     CDS             complement(1342..2361)
                     /locus_tag="VGT44_10280"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2581225.1"
                     /translation="MSKKKVRVGIVGTGNIAYVHEAGYTETSDLAQIVAVCDVNEAVA
                     RDRAAPYGARVYNDYRALVADAEIDLVDVTVPHRWHYEIALAALQQGKHVLVEKPMAM
                     TAEQALHLIQTAQAKGVKFTVAENTRFVAAYVEVERLLAAGAVGDIRFVRTYIAGSEV
                     ARIKSESSWVGSIENKGVLLDSGVHSFYLMRWLFGGVRDIQAATFKILSESRVDDTTI
                     ALGHLANGAVFETTQSCIVEAPWTERLEVHGSKGSLIVDHLANPTLVHFKGPDDVDGT
                     PVEAVPYEPLAWKYLSMIEEVKDFVQAIVDDRTPLIDPMYGYHAIQAVEAAYASIASN
                     SVVCF"
     gene            complement(2386..>3147)
                     /locus_tag="VGT44_10285"
     CDS             complement(2386..>3147)
                     /locus_tag="VGT44_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV2581226.1"
                     /translation="SGCPFYVLSKLQALVEPAAITSIDDLLLRQRLVKSEAELALMRK
                     ATKAVEMAVSALRGQITTGISRLALINTAKKLLLEHGATGIGHTTIAFGSSNPEIAFD
                     ESLAPGQVVTLDLGAVVDGYTSDNRRLLYSGPIPDDLRQLHRTMCGIVDQLGQALRPG
                     TPFADLYNLAVEIYATHELPPYFINIGHSIGLQTEEAWITFDSPYSVQAGMVLNIELY
                     TNYQDGSHIGDEETFLVTEHGAIKLTQTDPAIQSI"
BASE COUNT          735 a          848 c          857 g          707 t
ORIGIN      
        1 gattgatcag gccagagacc tgaatggcgt gggcgacatc agcggcggca gtgatatgca
       61 aaacgccctc taaataatag accccaaaga actggaagcc cgacactccc atatagatca
      121 tgaaactggc aagcacaaac caccagaaga aggagatgcc cgcccgcttc tggacgcgaa
      181 acatatctac aagcacctct ttcatcttta gtgtcttcat atcgggcagg ggcttgtcca
      241 gcttccatat cgccacaacg ttgacgacgg tcgagaccag gaagaagccc gcaatcacca
      301 taaaggcggc gtttcgtaga ttcgctgcga agagtgctgt gatcaggccc acaccgatag
      361 ccgagccgat taaggtgaaa acgcctttga tgcccgcgct cagaccccgc tgctctgtcg
      421 gcacaaagtc ggcgagcgtt ggctgccacg cgcctgaggc gacgttggtg aagatctgca
      481 tccccaggta ggccactgaa acagtgaaga gcgagagcgg gagaaagacc ggcatgagga
      541 tgaacggcag cgtcagcatc ataccccaga acatgatggg cctgcgtcgc ccaaagcgac
      601 tgtttgagtg atcgctgata atgcccgcga taatattgac gatgactgcg atgccaatcg
      661 ccacacctgc aagcacacca acggcaatgc ctttcgtcgc ctcagaggtc acggattgga
      721 tctgcaccgg caggatgaat atattgtagg cgagccagac caggtttacg cccagggcgt
      781 aggtgctggt taaggtgagc gtcggcaaag agagtttttt tgtgggagcc gccagttctg
      841 aatgaacgaa catcaccatc tccttagcgc tgtgcccgta tagaatcaca ccgcttgctt
      901 ttatctccag catgggtacg ttatccctga agggcaggga caaacatcgt agactgctag
      961 cggcggaggt gtttagagca ttaacttttg cagtttcaac aggtcttcct gcgctccctg
     1021 agccttgatc ttacgagtca tgaaggccgt catcgggttg atcttcttat ccataatgcc
     1081 agcaaggaca tctgtattgg caatcgtctt catatcagga tcggggatgg ctttttcttc
     1141 gagggttgct acaccattga caatagtaag cagaaacctc tgctgcgtgt cgggaaattc
     1201 aaattgcagg gttttggtga agtttttgaa tgtttcctgg agtgaaggat cgctaaaacg
     1261 gtcaaggata cgctccagat agggaaggat agtaaccatc aagtacaact cctttcaaaa
     1321 gagagcaata aggaaaagcg gtcaaaagca aacaacggag ttgctggcta tggatgcgta
     1381 ggctgcttca acggcctgga tggcatggta gccatacatc gggtcgatga gcggtgttcg
     1441 atcatcgacg atcgcctgca caaaatcctt tacttcctcg atcatcgaga gatatttcca
     1501 ggccagtggc tcatagggaa cagcctctac cggcgttcca tcgacgtcat ctgggccttt
     1561 gaagtgcaca agtgtcggat tggccaggtg atcgacaatc aacgatccct tacttccatg
     1621 aacctccaga cgctcggtcc acggcgcctc aacgatgcaa ctctgcgttg tttcaaagac
     1681 tgcaccgtta gccagatgac ccagagcgat ggtcgtatca tcaacccggc tttcggacag
     1741 aattttgaag gtggcggctt gaatatcgcg cacgccgcca aagagccagc gcatcaagta
     1801 gaaggaatgc actcctgagt cgaggagcac gcctttattt tcaatactgc cgacccagct
     1861 tgactcgctt ttgatgcgcg ccacctcact accggcaata taggtgcgca caaaacggat
     1921 atcacctacc gctccggcag ccagcaggcg ttccacttcc acgtatgctg cgacgaagcg
     1981 cgtattctcc gcgactgtga actttacgcc ctttgcctgc gcagtctgaa tcaaatgcag
     2041 ggcctgttca gcggtcattg ccatcggctt ttcgacgagt acatgcttcc cctgctgcag
     2101 cgcggccagg gcgatttcat agtgccagcg atgagggacg gttacatcaa ccaggtcaat
     2161 ttcggcatcg gcgacgagcg cacgatagtc gttatagacc ctcgcgccat agggggctgc
     2221 acggtcccgc gccactgctt cgttgacatc gcagacggcg acgatctggg cgaggtcgct
     2281 ggtctcagta tagccggcct cgtggacata ggcaatgttg ccagtgccca cgatcccaac
     2341 ccgcaccttc tttttgctca tgatgcttct ccttcgggag tattttcaga tgctttgtat
     2401 tgcggggtcc gtttgcgtta acttgatcgc tccatgctcc gttaccagaa aggtttcctc
     2461 gtcccctata tgagagccat cctgatagtt tgtataaagt tcgatgttga gcaccatgcc
     2521 cgcctgcacg ctataggggc tatcgaaggt gatccacgcc tcttccgtct gtaaaccaat
     2581 gctatggccg atgttgatga aatatggagg cagttcgtgg gtggcgtaga tctcgactgc
     2641 caggttgtaa agatcggcga aaggcgtacc tggccggagc gcctggccta actggtcgac
     2701 gataccgcac atggtacggt gaagctggcg cagatcgtct gggatcggcc cgctgtagag
     2761 tagacgtcgg ttgtcagagg tatacccgtc aaccactgca cccaggtcga gggtcacaac
     2821 ctggcctggg gccagcgact cgtcgaaggc gatttcggga tttgagcttc caaaagcaat
     2881 cgtcgtgtgc ccaatgccgg ttgccccgtg ttccagcagc aacttcttgg ctgtgttgat
     2941 cagggctaaa cgcgagatgc ccgttgtaat ctggccacgt aatgcgctga ctgccatttc
     3001 cacggccttc gtcgccttgc gcataagagc cagctctgcc tctgacttga cgagccgctg
     3061 ccgcaacaaa aggtcatcta tggatgtgat agcggctggc tcgacaagcg cctggagctt
     3121 tgacagcacg tagaaagggc agccgct
//
LOCUS       DASZAK010000586         2591 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_190785, whole genome shotgun sequence.
ACCESSION   DASZAK010000586 DASZAK010000000
VERSION     DASZAK010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190785"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="VGT44_10290"
     CDS             complement(<1..841)
                     /locus_tag="VGT44_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2581227.1"
                     /translation="MLKKPKPRFTIVFSFMTVLVLLLSACGAAGTPAPSPTTGQPVHG
                     GTWIDDIVAGPGSLLPQGSDTTYSVIIDQALYAPYFYGDDAGIVHPGIVSEIPTVANG
                     GATADLKTWTFKLRPNLKWSDGQPLTADDLAYSFKTFNDPTFGAKFTTGFADIMSTDV
                     SADKLSITMHLDKPIGNFVSNFVDANPGAPLPEHVFASMKPSEILKSPASQIPTVTNG
                     PFMIKTDETTAQQQYTVVRNPNYYRASEGLPYLDKIVFRIVNDPATILKDVQAGNITS
                     NWFL"
     gene            complement(1438..1509)
                     /locus_tag="VGT44_10295"
     tRNA            complement(1438..1509)
                     /locus_tag="VGT44_10295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1474..1476),aa:Arg,seq:ccg)
     gene            1961..2578
                     /locus_tag="VGT44_10300"
     CDS             1961..2578
                     /locus_tag="VGT44_10300"
                     /inference="COORDINATES: protein motif:HMM:NF022007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEV2581228.1"
                     /translation="MSENNRFQTHPFVIPSSPQYNSTPSFPTFPASQASPTSPLQQMS
                     QPYAAMTPPPLPVEPDDQTMMPSLPGAKITAPRPLWKRLVRYIAPAIALLLLGAIAFV
                     WFAPAAFTSSTPSSVTQQSFGNSPNTGGSGGTATGGDLHVYVVGAVRHPGVYDLPAGA
                     RVFQLIQAAGGALPGANLVALNMAAPLTDGQEVYQVKHNVISSVS"
BASE COUNT          607 a          719 c          694 g          571 t
ORIGIN      
        1 cgaggaacca gttggacgtg atattcccgg cctgcacgtc tttgaggatc gtagcaggat
       61 cgttcacaat gcggaacaca atcttatcca ggtatggcaa tccttcgctg gcgcggtagt
      121 agtttggatt gcgcaccacc gtgtactgct gctgagcggt cgtctcgtct gtcttgatca
      181 tgaacgggcc attcgtgacc gtcgggatct ggctggcagg agacttgagg atctctgaag
      241 gcttcatact cgcaaagaca tgctcaggca gcggagcgcc agggttggca tccacaaagt
      301 tggagacgaa gtttccaatg ggcttatcca ggtgcatggt gatcgagagt ttatcagcag
      361 agacatccgt agacataatg tcagcgaaac ccgtcgtgaa cttcgcacca aacgtcgggt
      421 cgttaaatgt cttaaagcta taggccaggt cgtccgcggt caggggctgg ccgtccgacc
      481 acttcaggtt cgggcgcagc ttgaacgtcc aggtcttcag gtccgccgtg gcgccgccat
      541 tggcgaccgt gggaatttcc gatacgatgc cagggtgaac gatacctgca tcgtccccat
      601 agaagtacgg agcatagagc gcctggtcaa ttatcaccga ataggtggta tctgatccct
      661 gtggaagcaa ggagcctggg ccggctacga tatcatcaat ccaggttccg ccgtgtacgg
      721 gctgccccgt cgttggagag ggagccggcg tacccgctgc tccacaagcg ctgaggagca
      781 ataccagcac cgtcatgaaa gaaaagacaa ttgtaaaacg cggcttgggt ttcttgagca
      841 tcgtgtcctc ctaaagcaag acaaacaaac tttggggaat cgtggtatct ctccgttgag
      901 ttccaactgt aaacgcacga tggcagcctg tcgtgctgcc agggattgaa cggcgctgaa
      961 caccatcccg gctacaagat atgatacagc gttagtaata ttacgctcat tttccaccac
     1021 atctcacacg tcttccagat ttgatcagtc aatcgagggg gaggttatgg cgtttcacgg
     1081 cttcggcttt tcccccacac ggtgagcaaa taccaaagcg cgcaaaactc gttggacatc
     1141 atctcaatct ccgcctcgcg atataaatgg tctactatga taatagcgtt tacagataag
     1201 acatgtttgc aatttaatac ctttaatttg ggtggtgagg gtaaacgaca aagacggagc
     1261 tatattgggg atgccgggta atttggcgtc tttagccctg agttgaggaa aatctacctg
     1321 atctctatta atgaatgccc tgtcggaaac ataagcctgc gccttataga tacgaacaat
     1381 caatggaaga atcagtatac ctcacaggtg cgatgggcaa aagattacga gatttaacgt
     1441 ccccgacagg aatcgaacct gcgcacgtgg cttcggaggc caatgctcta tccactgagc
     1501 tacggggact acatcacgaa tgataccaca gccgtcaacg catctcaagc ttgattcgca
     1561 aggcatccga cgatcgaaaa gtcacaaaga agaaacaaaa aggtccatcc tccagaggag
     1621 aatggatccg aactggctgc cagtgtctga ctagcgtgcc acgggctggc tgacgcgctg
     1681 caccagaagc cgcatagctt acgattactc gtatcaaatc gttaccgcct ctccaccttc
     1741 ctgctccaca aaatgcaggt aggcttcctc caggctcgga gaagcaggaa tggcctgggg
     1801 atgcggctgc gtgtgcgcaa gcaggcgcag ctcgacttgc tctccccggc gaatggcggt
     1861 actgatgggg taatcagaat tgatatgcgt tttctattga catgcatcga tctttcctgt
     1921 aatctagcgc ggaagagctt acgtcggagg gaaccgggac atgtcagaaa acaaccgctt
     1981 tcaaacccat ccattcgtca ttccatcctc gcctcaatac aacagcacgc catcatttcc
     2041 cacatttcca gcgtcgcagg catcgcccac gtcgccgttg cagcagatgt cccaacccta
     2101 tgcggccatg acaccgccgc ctctgcccgt cgagccggat gaccagacta tgatgccaag
     2161 cttgcccggc gcaaagatca ccgcgccgcg tcccttgtgg aagcggcttg tacgctacat
     2221 cgccccggcc atcgctctgc tgctgctggg agcgattgct tttgtctggt ttgcgcccgc
     2281 tgcattcacg tcctccacgc catcgagcgt tacgcagcaa tcgttcggca attcacccaa
     2341 cacgggcggt agcggcggca ctgccactgg cggcgatctg cacgtttacg tcgtgggcgc
     2401 ggtcaggcat cctggcgtct acgatctacc ggcgggcgcg cgggtcttcc agttgatcca
     2461 ggctgccgga ggcgcgctgc ccggcgccaa tctcgtcgcg ctcaacatgg ccgcgccctt
     2521 gaccgacggg caagaggtct atcaagtgaa acacaatgta atctcatcag tttcataatc
     2581 tcaggcggag c
//
LOCUS       DASZAK010000587         7204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_441992, whole genome shotgun sequence.
ACCESSION   DASZAK010000587 DASZAK010000000
VERSION     DASZAK010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7204
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441992"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1754
                     /locus_tag="VGT44_10305"
     CDS             54..1754
                     /locus_tag="VGT44_10305"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEV2581229.1"
                     /translation="MNRKTPRVNRFSQMLRFMRVSRLLLWTLWIIYRERRRVIRARER
                     GNYEVQPNIDVLINVLVAFRKTAIKLGVLMIKLGQFLSSRADLLPEEALSVISALQDE
                     VPAASFSHVVSVIESELGKPVEEVFSVLERKATAAASLGQVHKATLAATGETVAVKVQ
                     RPNIDQLVRSDLSTLKFVIWVITRFVDTSQFIDLKAVYREFKRTVYEEIDYVTEAANA
                     KRFKEMFKDDPTIYIPRVYEQYTTRRVLVLEWIEGIKINDYASLEAQGISRLEVAKRT
                     VQTYFYQFFDAGFFHADPHPGNIFVKKGAPGEGPIIEFVDFGMVGTLTKSMKKALKDL
                     FLGFLMRDSRALVSALSRLGFIGEGANMVAIERGMALMMEQYFGMTLGEARDLDIPEV
                     TQDAGRLLYGQPFQIPAQFAFTGRAIGTLVGVSTGLAPEFNFVDVATPYARKFLGLDA
                     EGASQTIQSILNQLLETGRILLKLPADLERVITRLETGQIEVKTESSAQAGQSRRRGR
                     NGNHRNGNGGGSGALASVVTFMASLGGGILLTNAHQMLPGWIFMGLAGLMGLRLLFRR
                     "
     gene            1797..2021
                     /locus_tag="VGT44_10310"
     CDS             1797..2021
                     /locus_tag="VGT44_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581230.1"
                     /translation="MPQSPFIRLLPTGEEMVRFRYRSIELHKMTASELKLTGLVGLAA
                     LLPLTSDGARREVIEDMVAQLVAAEKMDSL"
     gene            complement(2234..3043)
                     /locus_tag="VGT44_10315"
     CDS             complement(2234..3043)
                     /locus_tag="VGT44_10315"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2581231.1"
                     /translation="MASPYTIILAPNPSIMTGPGTNSIILGGRPGGAIVIDPADDNND
                     HLNALIKEGELRGGIRRILITHGHPDHVGGAAALRERLHIPILAYNRRGVPIADEELA
                     DGATLPVGDDTLRSIHTPGHRFDHLSFYLERQRILFAGDLISGITTNVIAPPDGDMFD
                     YLASLQRLQPLDIAEIVPGHGPTIADAQARIAEYIAHRRLREQQILTTLETLPRGTSI
                     PDMVPAIYTDVDPKLHPIAAWSVEAHLLKLEKEGLVERIGEKGWALVEPTA"
     gene            complement(3061..3882)
                     /locus_tag="VGT44_10320"
     CDS             complement(3061..3882)
                     /locus_tag="VGT44_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581232.1"
                     /translation="MLVQPRLASAVMLLRDRPAEQGIEVFMVRRVIQSDFMPDVYVFP
                     GGSISKDDNAAELTRNICASVDPLPSDPQGHTALGRGMRAAAIRELFEEAGVLLAYHN
                     DAIISIPAEDVARFAPYRQAFQQRGGSLVAMARAEGLTLATDRLGYFAHWITPEGMPK
                     RFDTHFFITTAPSEQETAHDQLETSEGIWIAPIDALAGYKQGTFPLVFATIYQLRELA
                     AFNSVEAALAHSATQPVPTRIPILEMQDGGPARVHLAGEDDDQWDVPEHMRRPKQ"
     gene            complement(4004..4495)
                     /locus_tag="VGT44_10325"
     CDS             complement(4004..4495)
                     /locus_tag="VGT44_10325"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2581233.1"
                     /translation="MASVENPFDKVRRKMVTARIEFMGQLAKFSANELTQPPVDSNEW
                     TPLQIANHLYIADALALEQIRLVQEEDNPLIVETEEETPRLTRAAPPPTSLDSVLAGM
                     AARREAVFEYLSQLPDDAWERPFRHPTWGERKFYQLVNVLPLHDQIHARQLADIKAAN
                     GNS"
     gene            complement(4513..5511)
                     /locus_tag="VGT44_10330"
     CDS             complement(4513..5511)
                     /locus_tag="VGT44_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV2581234.1"
                     /translation="MAKAKPGLGRGLDALLSGASDVMSAQRATRVSEESIAQIPIESV
                     IPNPRQPRRAFREDDPKLLELSASIKQHGLLQPLVVARLESSESLASDWFSDSSGGQS
                     NAQLSSPVIRYQIIAGERRWRAARLAGLTSVPVVVKEATPQKMLELALIENIQRADLN
                     PIEEALAYQSLIEEYGLTQEMVARQVSKDRSTITNALRLLQLPPRVREALINQLEHFT
                     EGHARALVGIAREEDQLAAMGKIITMKLNVRQSEELAARIKAADRADAASGQKPLAPN
                     RRSPDIEDLETQFRNSLMVKVDLKRNTKGKGTLVLHFNNEEELDRLYTRLIHHSDP"
     gene            complement(5504..6274)
                     /locus_tag="VGT44_10335"
     CDS             complement(5504..6274)
                     /locus_tag="VGT44_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEV2581235.1"
                     /translation="MSKVYAITNQKGGVGKTTTAINLATYLAVAGRRVLLVDMDPQSN
                     TTSGIGVARHTRPHTIYDLLVQEDDELNIFDVIVPTKRQELVVAPCTVDLAAAEIELV
                     GTLARENRLRQVLEPIRTRCDYVIIDCPPSLGLLTVNALVAADGLIIPIQCEYLALEG
                     LTHLLNTIQIVKTKLNPDLYIAGVVLNMFDPRTRLAGEIVREVRTHFPKEVFQTIINR
                     NVRLSEAPSFGQSIVDYDPGSPGATAYRSLAEEVMARG"
     gene            complement(6385..7098)
                     /locus_tag="VGT44_10340"
     CDS             complement(6385..7098)
                     /locus_tag="VGT44_10340"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEV2581236.1"
                     /translation="MSTTQAAAALYRLQQLDLELERLAAEQQALVRSLQEDTTLNKAR
                     EEQQAAQYQLQVSLRLQEEAERALNELNTRLRALDQRLYSGMITNPKELNAVQQEAQH
                     LRIQQNRQEELTLELMDATDTLNANVKQKKEARQQVEQARQQSNVALVARRDQLQRKQ
                     QEAQEKREQFAATINSELVKRYDMLRRSRQGRAVSRVEQQSCQWCRVILTPSELQHAR
                     ASQELQTCSNCGRILYFDR"
BASE COUNT         1344 a         1998 c         2278 g         1584 t
ORIGIN      
        1 gcctggaacg tacatgttat gcttatagag agagatttga aaggcgtaca ctgatgaatc
       61 gcaaaactcc tcgtgtcaat agattttcgc aaatgctgcg tttcatgcgc gtctcacgct
      121 tgctgttgtg gacgctgtgg atcatctacc gcgagcgccg ccgcgtcatc cgcgcccgtg
      181 agcgcggcaa ctacgaagtg cagcccaata tcgatgtgct gatcaatgtg ctggtggcat
      241 ttcgcaagac ggccatcaag ctcggtgtgc tgatgattaa gctggggcag ttcctcagct
      301 cgcgcgccga cctgctgccg gaagaagcgc tttccgttat cagcgcgctg caggacgagg
      361 ttccggcggc gtcgttcagc cacgtcgtct cggtgatcga gtccgaactg ggcaaaccgg
      421 tcgaggaggt attcagcgtg ctggagcgca aggcgacggc ggcggcctcg ctcggccagg
      481 tgcataaggc caccctggcc gcgacgggcg aaacggtggc cgtcaaggtg cagcgtccca
      541 atatcgatca gcttgtgcgc agcgacctca gtacgctcaa gtttgtgatc tgggtcatca
      601 cccgcttcgt cgatacgagc cagttcatcg atttgaaggc ggtttatcgc gaattcaagc
      661 gcactgttta cgaagagatc gattatgtga ccgaggcagc caacgccaaa cgcttcaagg
      721 agatgttcaa ggacgacccg accatctata tccccagggt gtacgagcaa tatacgacac
      781 gccgagtgct ggtgctggaa tggatcgagg gcatcaagat caacgactac gccagcctgg
      841 aagcccaggg catcagccgc ctggaagtcg ccaagcgcac cgtgcagacc tacttctacc
      901 agtttttcga tgccggtttc ttccacgccg acccgcatcc aggcaacatc ttcgtcaaga
      961 agggcgcgcc cggcgaaggg ccgatcatag aattcgtcga tttcggcatg gtcgggacgc
     1021 tcacgaagag catgaagaag gcgctgaaag acctcttcct gggtttcctg atgcgcgact
     1081 cgcgcgccct ggtcagcgcg ctctcgcgac tgggttttat tggcgagggc gcgaacatgg
     1141 tggccatcga acgcggcatg gcgctgatga tggaacaata ctttggtatg acgctgggcg
     1201 aggcgcgcga cctcgacatc ccagaagtga cgcaggacgc cggtaggtta ctctacggcc
     1261 agcccttcca gattcccgcg cagttcgcct tcactggccg cgccatcggt acgctggtgg
     1321 gcgtctcaac cggcctcgcg ccggagttca acttcgtgga tgtcgccacg ccctacgcgc
     1381 gcaagttcct gggtctcgac gccgaaggcg caagccagac cattcagagc atcctgaacc
     1441 agcttttaga gacaggccgt atcctgctga agctgcccgc tgacctcgaa cgagtcatca
     1501 ccaggctgga aaccggccag atcgaggtca agaccgaatc cagcgcgcag gcgggccaga
     1561 gccgccgtcg cggtcgcaac ggcaatcacc gcaatggcaa cggcggcggt tccggcgcgc
     1621 tggcctccgt cgtaaccttc atggcctctc tcggcggcgg catcctgctg accaacgccc
     1681 accagatgct accagggtgg atctttatgg ggttggcggg gttgatgggg ttgcggttgc
     1741 tattcaggag atagctaatt cattcatgcg taatttatct gaaagattgt ggcaatgtgc
     1801 cgcaatctcc ttttatccgg cttttgccaa caggtgagga gatggtgcgt tttcgctatc
     1861 gcagcatcga actgcataaa atgactgcga gcgaactcaa gctaaccggg ctggtagggt
     1921 tagctgcgct tctaccgttg acgagcgatg gtgcgcggcg tgaagtaatc gaggatatgg
     1981 ttgcccaact ggttgcagca gaaaaaatgg attcgttgta ggttgggtat gcgttagctg
     2041 cgaaagtaat ggcagacgat ttacaatggc taagaaggag gttcgccgtg ttcagtgatt
     2101 ttctacgaga ttcgcctgta taccaggaag tcctggaaga gggcttggag aaaggtctgc
     2161 agactggaga tctgagagcg cagcgacaga cccttcgtgt tgaccagcgc acagccattc
     2221 caatccaggc atcttacgcc gtcggttcaa ccagcgccca ccccttttca cctatcctct
     2281 ccaccaatcc ctctttctcc agcttcagca ggtgtgcttc tacggaccag gcggcgatgg
     2341 gatgcagctt cgggtctacg tcggtgtaga tggctggcac catgtcgggg atggatgtgc
     2401 cgcgtgggag ggtttccagg gtggtaagga tctgctgctc gcgcaggcgg cggtgggcga
     2461 tgtattcggc gattctggcc tgggcgtctg ctatggtcgg gccgtgaccg gggacgattt
     2521 cggcgatatc gaggggttgc aggcgttgca gggaagccag gtagtcgaac atgtctccgt
     2581 ctgggggcgc gatgacgttg gtggtaatgc ctgagatgag gtcgccggcg aagaggatgc
     2641 gctgccgctc caggtagaag ctgaggtggt cgaagcgatg ccccggcgtg tggatagagc
     2701 gcagggtatc atcgcccacc ggcagcgtcg cgccgtccgc cagttcttcg tcggcgatgg
     2761 ggacgccccg gcgattgtag gccaggatgg ggatatgcag ccgctcgcgt agggctgccg
     2821 cgccgccgac gtggtcggga tggccgtgcg tgataagaat gcgacggatg ccgccgcgca
     2881 gctcgccctc ctttataagt gcattgagat gatcgttatt gtcgtctgcc gggtcaatca
     2941 cgatggcccc gcccggtcta ccgcccagga tgatcgagtt ggtgcctggc ccggtcatga
     3001 tggagggatt gggagcaagg ataatggtgt acggtgatgc cacgaagtgc ctttctatct
     3061 ctactgtttc ggacggcgca tatgttccgg cacgtcccat tggtcatcat cctcacctgc
     3121 gaggtggacg cgcgccgggc cgccgtcctg catttcgaga atggggatgc gcgtcggcac
     3181 gggctgtgtc gcggaatgcg ccagggctgc ctcgacgctg ttgaaggcgg cgagttcgcg
     3241 caactggtag atggtggcga agacgagcgg gaaggtgcct tgcttgtagc ccgcaagcgc
     3301 gtcgattggc gcgatccaga tgccctcgct ggtctccagt tgatcgtggg ccgtctcctg
     3361 ctcagatggc gcggtagtga taaagaaatg agtgtcgaag cgtttgggca tcccctccgg
     3421 cgtgatccag tgtgcgaagt agcccaggcg gtcggtcgcc agtgtcagcc cttcggcgcg
     3481 cgccatcgcc acgagcgatc ctccgcgctg ctggaaggct tgccggtaag gtgcgaagcg
     3541 cgctacatct tcggcaggaa tagagatgat tgcgtcgttg tgataggcca gcagcacgcc
     3601 tgcctcctcg aagagttcgc gaatggcagc ggcgcgcatc cccctgccca gcgcggtgtg
     3661 cccctgcggg tcggagggta ggggatcgac ggaggcgcaa atatttctgg tcagctcggc
     3721 ggcgttatcg tctttcgaga tggaaccgcc ggggaagacg tacacgtccg gcatgaaatc
     3781 gctctgaatg acgcgccgca ccatgaatac ttcgatcccc tgttcggcgg gccggtcccg
     3841 caacaacatc acggctgagg ccagccgggg ctgtacaagc ataggatggg tctccaatct
     3901 gtcttctcac gaactccgta ggtgccgatt gatcgcgcac accgccgatt gatcggcctg
     3961 ctgcattgcc gtcatcgccc aggactggcc gatcaatcgg cacctacgag tttccatttg
     4021 ctgctttgat gtcggcgagt tgccgggcgt gaatctggtc gtgcagggga aggacgttga
     4081 cgagctggta gaacttgcgc tcgccccagg tggggtggcg gaaggggcgc tcccaggcat
     4141 cgtcgggaag ctgcgagagg tattcaaaga ccgcttcgcg gcgcgcggcc atgccggcca
     4201 gcacggagtc gagcgaggtg ggtggcgggg ccgcacgggt cagacgaggc gtttcttctt
     4261 ccgtctcgac gatcaagggg ttgtcttctt cctggacaag cctgatctgt tcgagcgcca
     4321 gagcgtcggc aatgtagagg tggtttgcga tctgcaaagg cgtccactcg ttgctatcga
     4381 cgggcggctg tgtcaattcg ttggcgctga acttcgcaag ctggcccata aattcgatgc
     4441 gcgctgtgac cattttgcgg cgtactttgt caaaggggtt ctccactgat gccatgtctg
     4501 ttctccctct cgctacgggt ctgaatggtg tatcaatctt gtgtataaac ggtcaagctc
     4561 ttcctcgtta ttgaagtgta gtaccagggt acctttgcct ttggtgttgc gtttgagatc
     4621 gaccttgacc atcagcgagt tgcggaactg cgtctcaagg tcttcgatat caggcgagcg
     4681 gcgattgggc gcgagcggct tctgaccgga tgcggcgtcg gcgcggtcgg cggctttgat
     4741 gcgtgccgct agttcttcag actggcgcac gttcagtttc atggtgatga tcttgcccat
     4801 ggcggccagc tggtcttctt cgcgcgcgat gccgaccagg gcgcgggcgt gaccctcggt
     4861 aaaatgctcc agttgattga tgagggcttc gcgcactctg ggcgggagtt gcagcaggcg
     4921 tagggcgttg gtgatggtcg agcggtcctt gctgacctgg cgcgccacca tctcctgtgt
     4981 caggccgtat tcttcgatga gcgactggta ggccagcgcc tcttcaatgg ggttgaggtc
     5041 ggcgcgctga atattctcga tcagggctag ttcgagcatt ttctgcggtg tagcctcttt
     5101 gaccactacc ggtacgctgg ttaaacccgc cagccgagcg gcgcgccagc gacgttcgcc
     5161 cgcaatgatc tgatagcgga tcacaggcga agacagctgg gcgttggatt gccccccgga
     5221 ggaatcagaa aaccagtcgg aggcgaggct ctcactgctt tccaggcggg ctacgacgag
     5281 aggttgaagc agaccgtgtt gtttgatcga cgcgctcaac tctaacagtt tcgggtcgtc
     5341 ttcacgaaag gcgcggcgcg gctggcgcgg gttgggtatg acgctctcaa taggtatctg
     5401 cgcgatgctt tcttcgctga cgcgggtggc acgctgggcc gacatgacat ccgatgcgcc
     5461 ggagagcagt gcatccagcc cgcgccccag tcctggtttt gctttagcca cgagccatca
     5521 cctcctctgc caatgagcgg tacgcagtcg cgccgggaga gcctggatca taatcgacga
     5581 tggactggcc gaagctggga gcctcgctca ggcgcacatt gcggttgata atagtttgaa
     5641 aaacttcctt tgggaagtgc gtgcgcacct cgcgcacgat ctcgcccgcc aggcgtgtgc
     5701 gcgggtcgaa catattcagt acgacgccgg cgatgtacag gtcggggttg agcttggttt
     5761 tgacaatctg gatagtattc aaaagatgcg tcaagccttc cagcgccagg tattcgcatt
     5821 ggataggaat gatcaagcca tcggcggcga ccagggcgtt gacggtgagc agccccagcg
     5881 atggcgggca gtcgatgatg acgtaatcgc agcgtgtgcg aatcggctcc agcacctggc
     5941 gcaggcgatt ttcacgcgcc agcgtcccca ccagttcgat ctctgccgcc gccaggtcaa
     6001 ccgtgcaggg agcgacgacc agttcctggc gtttggtagg aacaattacg tcgaagatgt
     6061 tcagttcatc atcttcctgc accagcagat cgtagatcgt atgcgggcgg gtatgcctgg
     6121 ccaccccgat gccacttgtg gtattcgatt gcgggtccat atcgaccagc aacacccggc
     6181 gaccggcgac cgcgagatac gtagccaggt tgatagcggt ggtcgttttc cctacgccgc
     6241 ctttctgatt ggtgattgca tagactttgg acacccgttg accttatacc tttcaaggct
     6301 tattctggcg actatcgcca ggcgaaacat acccgcgcag caggtacacc cattgtaaca
     6361 aaagcaacca gcagaagcaa cctgttagcg gtcgaaatag aggatacgcc cgcagttgct
     6421 gcacgtctgc aattcctggc tggcgcgcgc gtgttgtagc tcgctcggcg tcaggataac
     6481 gcggcaccat tggcaggatt gctgctctac gcgcgaaaca gcgcgcccct gcctggaacg
     6541 tctcagcatg tcgtagcgtt tgaccagttc gctgttgatg gtggcggcga attgctcgcg
     6601 cttctcctgc gcttcctgct gcttcctttg cagttgatcg cggcgcgcaa ccagcgcaac
     6661 attactctgc tgccgcgctt gctcaacttg ctgccgcgcc tcctttttct gctttacgtt
     6721 cgcgttcagc gtatcggtcg cgtccatcaa ctccagtgtc agttcttcct ggcgattttg
     6781 ctggatgcgc aagtgctgcg cttcctgctg cactgcattg agttctttgg gattggtaat
     6841 catgccgcta taaagccgtt ggtcgagggc gcgcaagcgg gtgttcagtt cattgagcgc
     6901 tcgctcagcc tcttcttgca gccgcaagct gacctggagt tgatactgtg cagcctgttg
     6961 ttcctcgcgc gctttgttga gtgtggtatc ttcctggaga gagcggacca acgcctgctg
     7021 ctcggcggcg aggcgttcga gttcgaggtc aagctgctga agtcgataga gcgcagcggc
     7081 ggcctgggtt gtactcatgg atgtcccttc catttccagg gatgaaaaca atctgcacgg
     7141 gcggttgggg ttagagtacc attgtacaac cctgctggaa ctggttgcaa ggtgaaaaag
     7201 aggc
//
LOCUS       DASZAK010000588         2177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_654798, whole genome shotgun sequence.
ACCESSION   DASZAK010000588 DASZAK010000000
VERSION     DASZAK010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654798"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(239..751)
                     /locus_tag="VGT44_10345"
     CDS             complement(239..751)
                     /locus_tag="VGT44_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV2581237.1"
                     /translation="MISPLLYFLLFLKASLFSTGGFSNLPSLHQDLIANHWATEADFG
                     QSIAIGQISPGPNGLWVISMGYLTYGITGALLALVAITLPALLVLAVSAGYTRIEHRP
                     SVQGAMYGVSLAVVGLLLTVVWTILHQPGVDWRGLLIAAAAFGLALTRKVNLLLILAL
                     AGVVGFLLYR"
     gene            complement(748..1365)
                     /locus_tag="VGT44_10350"
     CDS             complement(748..1365)
                     /locus_tag="VGT44_10350"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEV2581238.1"
                     /translation="MALQDPRPGKWQLFRIWAIIGLQSFGGGASTQFLVQHTFIDKHR
                     WLSMEEYTHLWSLCVLTPGINLVALTVLIGKKLGGFWGIVVSLTGMLVPSAAITCALA
                     ATFQLFEHNVSVQAVVQGIIPATGGIMLLVGFNFARPLITRGIKEGVIFLLGSAAITA
                     ACALVIILLKVSVIVVVLVAALLGIIFFSSRAPAPLLNEGEDRRP"
     gene            1571..2110
                     /locus_tag="VGT44_10355"
     CDS             1571..2110
                     /locus_tag="VGT44_10355"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581239.1"
                     /translation="MLLRRSFYVTIGLVIAMFVAACGSSTTGTTPSAGSGGSTGNTTI
                     VVKTASVSVKGKMEAVLTNTQGKTLYYFTADTATTTACSGSCASMWPPLLNSGTATPA
                     SASALSGTLTALTDANGSQVTYNGHLLYAFSGDTAAGQANGEGLFGSWFVATPNLAVL
                     GGSTQPSSGGSSGGGYGYR"
BASE COUNT          485 a          626 c          673 g          393 t
ORIGIN      
        1 ataccggcct cgatcttccc aaagacgacc ctcgcctgca acagacctat gcctggctca
       61 tgcaggaatt cgacaacggt cgccagacaa gcgtcgaatg ggaagaactc gcccccatct
      121 ttgaaggcaa accgtcgtag ggacaggggg agtggatcgt gacctccgac gcggacaggc
      181 acaaggcccc cgccgctccg ccccaccccc ccctgtgtcc ctacggaatg ttagcaggtt
      241 agcgataaag gagaaatcct acaacccctg ccagggcgag aataagcagg aggttgacct
      301 tgcgggtcag ggccaggccg aaggcggcag cagcgatcag cagacctctc caatcgacgc
      361 cgggttgatg caggatcgtc cagacgacgg taagcagcag gccgacgacg gcgagcgaga
      421 cgccgtacat cgcgccctgc acggaggggc ggtgttcgat gcgcgtgtag ccggcggaga
      481 cggccaggac gagcagcgcg ggcagcgtga tggcgaccag ggcgagcagc gcgccggtga
      541 tgccgtaggt caggtagccc atactgatga cccacaggcc attgggtccg gggctgatct
      601 ggccgatggc gatggattgc ccgaagtcgg cctcggtggc ccagtggttg gcgatcaagt
      661 cctggtgcag gctgggcagg ttgctgaagc cgccggttga gaagagcgag gctttcagga
      721 agagcaggaa gtagaggagc ggtgagatca tgggcgcctg tcctcgcctt cgttcagcag
      781 gggagcggga gcgcgcgacg aaaagaagat gatgcccagc agcgcggcga caagcacgac
      841 cacgatgacc gagaccttca gcagaatgat gaccagggcg caggctgctg tgatcgcggc
      901 gcttcccagc aaaaagatca cgccttcctt aatgcctctt gtgatgagcg ggcgcgcaaa
      961 attgaacccg accagcagca tgatgccgcc cgtcgcgggt atgatgccct gcaccactgc
     1021 ctgaaccgat acgttatgct cgaaaagctg gaaggtggcg gccagcgcgc aggtgatcgc
     1081 cgcgctcggc accagcattc cggtcagcga gacgacgatg ccccaaaagc cgcccagctt
     1141 cttgccgatc aggaccgtca gcgcgacgag gttgatgccc ggcgtcagca cgcataagct
     1201 ccagaggtgg gtgtattctt ccatcgacag ccagcgatgt ttgtcgatga aggtatgctg
     1261 taccaaaaac tgggttgatg ctccgccccc aaaggattgc agcccgatga tggcccagat
     1321 acggaagagt tgccatttgc cgggtcgggg gtcttgtaat gccatagaac ttgctccaat
     1381 gctcaatatc ctctacagtg taacccacct ctgtatttct cttcagagaa aagagtgcat
     1441 cccatgctga aaaacactat cgcatgttct atatgcagat gagatgctgt ctggtgagtt
     1501 gttcgtagcg cgtaaactca ataaaactat catacacttt taaaagttgt gtagacaagg
     1561 agaatccagt atgcttctca ggcgttcttt ttacgtcacg attggccttg tgattgccat
     1621 gttcgtcgcg gcctgtggca gttcgacgac cggcacgacc ccttcggcgg gttcgggtgg
     1681 ctcgacaggc aacactacta tcgtcgtcaa aacggcgtcg gtcagcgtga agggtaagat
     1741 ggaggcagtt cttaccaata cacagggcaa aacactgtac tactttacgg cggatacagc
     1801 gacaacgacg gcctgctccg gcagttgcgc cagcatgtgg ccgccgctcc tcaatagcgg
     1861 cacggctaca cctgccagcg cgagcgccct ttcggggacg ctgaccgcgc tgacggatgc
     1921 caacggcagc caggtcacct ataacggcca cctgctgtac gccttctccg gcgacacggc
     1981 tgccggccag gccaacggcg aggggctgtt cggttcgtgg ttcgtcgcta ctcccaatct
     2041 cgccgtcctg ggcggcagca cgcagcccag cagcggcgga tcatcgggtg gcggatacgg
     2101 gtacaggtag cagcacatga tagcatggcc gaatcaggat catgtaaacg ttcaggcatg
     2161 acgcaggatc atgtaaa
//
LOCUS       DASZAK010000589         3779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_264222, whole genome shotgun sequence.
ACCESSION   DASZAK010000589 DASZAK010000000
VERSION     DASZAK010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264222"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1495)
                     /locus_tag="VGT44_10360"
     CDS             complement(131..1495)
                     /locus_tag="VGT44_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2581240.1"
                     /translation="MPTSSQIREIEWDVVGDEVITYVRNLLRLDTRNPPGNETRAAEY
                     LRGILEREGIACEIVGPGPDRGTIVGRLKGDGSAPPLLLMSHTDVVAVEPEKWTHDPF
                     AAEIDDGFIYGRGAMDMKHMVTMELMTILLLKRAGVPLKRDVIFMAAADEEVGGHQGA
                     GWVAHHRPELIQAEYALNEGGGNAIEINGRRYYTVQTAEKGAARFRLRAKGKPGHGSV
                     PHEDNAITKLVEVLSRLGDRQPPVHFSASFRGLITGIASAQPPEIAQLILAILANEQT
                     ADVAIDALPMEDLLKQEMRAMIRNTIAPTVLRAGSQINVIPSEAEALLDGRIVPGCTT
                     EMFLGEIRAIFGEDADIEFIDPNEALEADPASPLFEVIKDVMREHDAEATVVPTLLVG
                     GTDAQRVAGLGTKVYGFAPEMYISGLNDWDRIHGHDERINIRSLQWGTRVLYDVVARF
                     ACSD"
     gene            1722..2720
                     /locus_tag="VGT44_10365"
     CDS             1722..2720
                     /locus_tag="VGT44_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2581241.1"
                     /translation="MHTVATIVYDDVNPFELAVATEVFGFERPELGVPWYRFLICAEE
                     PRPIRTSIGILLTTPYSLEQLPLADTIIIPSSRPGIVPVSKELLETLRQSYQRGARLI
                     AFCTGAFILAASGLLDGRRVTTHWAWATELAERYPRVQVDPQKLYIDEGQVLTSAGTA
                     AAIDLSLHVVRQDYGAEIAAAVARRMVVSPHRDGGQAQYIETPLLSVPEEGETFGETL
                     TWMVAHLHEELTVEQMAARAVMSPRTFARRFRTTLGTTPYQWLLKQRIVLAQRLLETT
                     NETVERVAMSCGFSSAATLRLHFQRLLYISPQMYRNAFHRENRGETARRSERRVGI"
     gene            2744..3673
                     /locus_tag="VGT44_10370"
     CDS             2744..3673
                     /locus_tag="VGT44_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2581242.1"
                     /translation="MLQHENQGNLRFLVPLDGSRLAESVLPVVQQVAGRFHAQVTLLH
                     IVEEHPPTAIHGEPHLTGVAQAQAYLEEIATRLRSSGIVVEIHVHQEKEDNVARSIVQ
                     HSQEMNVDLVIMCTHGHGGLRELLFGSKAQQALQQGTQSILLLFPREDGSAYPFNLQR
                     ILVPLDGTAAHEPALPIAITVARTFGAELHLVLVIPTLSTLSGDRAASGLLLPTTMRA
                     LLDLSQQDAADYLEQAIARCRAEGVVAHAELLRGDIVPVVLGLAERLNVDLIVLASHG
                     RTGLDALLTGSVASRIAGRRIRPLLLVRARQPA"
BASE COUNT          812 a         1074 c         1028 g          865 t
ORIGIN      
        1 taattacgcc cgcctcttgt gggagactga acgtggagat gaagcgctac aacacgcggc
       61 tcgcgccagg gccatttggg ataagcgagc ggggtaaaga gttcatcgat agtctgctat
      121 ttaacgttct ctagtcggaa caggcgaacc gcgctacaac atcgtagagc actcgcgtgc
      181 cccattgcag cgaacggata ttgatccgct catcgtggcc gtgaattcta tcccaatcat
      241 ttaatccact gatatacatt tctggcgcga agccgtacac cttcgttccc agccctgcaa
      301 cgcgttgggc atccgttccg ccgacgagca gcgttggaac cacagtggct tcagcatcgt
      361 gctcgcgcat cacatcctta attacctcga aaagcggcga ggctggatca gcctccagcg
      421 cctcattagg atcgataaac tcgatgtctg catcttcacc gaaaattgcg cgtatttccc
      481 cgagaaacat ctccgtcgta caacccggaa cgatacgacc atccagtaat gcctctgctt
      541 ctgagggaat cacattgatc tgggagccgg cccgcagcac ggtgggtgcg atagtgttcc
      601 gtatcattgc tcgcatttcc tgcttgagga ggtcttccat cggcagcgcg tcaatggcga
      661 catcggcagt ctgctcgttc gcgagaatgg ccaggatgag ctgggcgatc tccggcggtt
      721 gcgcgctggc gatgcctgtt attaatcctc gaaaggaagc gctgaaatgg actggcggtt
      781 ggcgatcccc taatctgctg agcacttcca cgagcttggt gatggcgtta tcctcgtgcg
      841 gcaccgagcc atgccctggc ttgccttttg cacgcagccg gaaacgcgca gcgccctttt
      901 ctgctgtctg tacggtatag tagcggcgcc cgttgatttc aatagcgttc ccgccccctt
      961 cgttgagggc atattcagcc tgaattaact ctggacggtg gtgagctacc cagcctgccc
     1021 cctggtgtcc tccaacctcc tcgtcagcag ccgccataaa gatcacatca cgtttcaggg
     1081 gcacacctgc tcgtttcagc agcaatatag tcatcagttc catcgtaacc atatgcttca
     1141 tatccatcgc gccgcgccca taaataaacc catcatcaat ctcggctgca aatggatcgt
     1201 gcgtccactt ctcaggctcg actgccacga catccgtatg tgacatcaac agtagcgggg
     1261 gcgcagaacc atcgcccttg agccgcccta caatcgtccc gcgatcaggg ccgggtccca
     1321 ctatctcgca ggctatcccc tctcgttcga gtatcccgcg caggtattca gccgcccggg
     1381 tttcgttgcc tggagggttg cgcgtgtcaa ggcgcaggag attgcgtaca taggtaatca
     1441 cctcatcccc taccacgtcc cattcgattt ctctgatctg gcttgaagtt ggcatcaatg
     1501 tctcctcaat agtgttcggc tgccgtccgt gtttaggaac tgagcctctt cgatgatgaa
     1561 cgatgggggt accattgagc acattatagt gcttgagaga tatttctggc aaatgccata
     1621 ttcacacaag catcgcttgg cagcaaatga tctatatgct acaattctgc cataaatgga
     1681 atgctactgg ctgaaaagat aaggagcggc aaataaccgc tatgcacacc gttgcaacta
     1741 tcgtgtacga tgacgtcaat ccttttgaat tggctgtggc cacggaagtc tttgggtttg
     1801 aacgtcccga acttggcgtg ccctggtatc gcttcttgat ctgtgctgag gagccaaggc
     1861 cgattcgaac ctcgatcggt atcttgctca ccaccccgta ttcccttgag caacttcccc
     1921 tggcggatac gatcattata cccagttcgc gccctgggat tgtcccggtt tccaaggaat
     1981 tgctggagac cttacgccag tcctatcaac gaggagctcg attgatcgcg ttctgtacag
     2041 gggcatttat cctggctgcc tcggggctgc tggatggtcg ccgggtcact acgcactggg
     2101 catgggcaac agaactagcg gagcgctatc ccagggtgca ggtcgatcct cagaagctgt
     2161 acattgatga gggtcaggta ttgacttctg caggaacggc tgctgcgatt gacctctcac
     2221 tgcacgtggt gcgccaggac tatggagccg agatcgcagc agcggtagcc aggcggatgg
     2281 tggtttctcc gcatcgcgat ggtggtcagg cacagtatat cgagacgcct ttgctctctg
     2341 taccggagga gggtgaaaca ttcggcgaaa cactgacatg gatggttgcc catctgcacg
     2401 aagaacttac ggtagagcag atggcggctc gggcagtgat gagcccacgg acgttcgcgc
     2461 gtcgcttccg tacaaccctt ggcacgaccc cttatcaatg gctgctcaaa caacgcattg
     2521 tgctggctca gcgcctgtta gagacgacaa acgagacggt tgagcgagtg gcgatgtcct
     2581 gtggcttcag ttcggcagcc acgctgcgcc tgcactttca acgtctcctc tacatatcac
     2641 cacagatgta tcgcaacgcc tttcaccgcg agaacagagg cgaaacagca aggcgatcgg
     2701 agcgaagagt tggcatctag acattctttg gaggttgatg acaatgctac aacatgaaaa
     2761 tcaaggcaat ctccgttttc ttgtcccgct cgatggctcg cgcctcgcgg agtcagtgct
     2821 acctgtcgtc cagcaggtgg caggccgttt ccacgcgcag gtgaccttgc tgcatatcgt
     2881 ggaagagcat cccccaacag ccattcatgg ggagccgcac ctgaccggtg ttgcccaggc
     2941 acaagcctac ctcgaagaaa tcgcgacaag attgcgctcc tctggcattg tggttgaaat
     3001 acacgtgcat caagaaaagg aagacaatgt tgcgcgcagc atcgtccagc attctcaaga
     3061 gatgaacgtg gacctggtca tcatgtgtac gcatggtcac ggtggactgc gggagcttct
     3121 ttttggcagc aaggcgcagc aggcgcttca gcaaggcact caatcaatcc tgctcttgtt
     3181 tccgagagag gatggctctg cctacccttt caatctccag cgcatcctcg tcccgctgga
     3241 tggtacagcc gctcacgaac ctgcgcttcc tatagccatt actgtagctc gcaccttcgg
     3301 cgcggaacta cacctggtgc tggtgattcc aaccctgtca acgctctcag gggatcgagc
     3361 ggcgtctggt ttactactcc ccactaccat gcgggctctg cttgacctgt cacaacagga
     3421 cgccgcggac tacctggaac aggccatagc tcgctgccgc gcggaaggcg ttgtggccca
     3481 tgctgagctc ctcaggggcg atatcgtacc tgtggtgctt ggccttgccg agcgattgaa
     3541 cgttgacctg attgtgctgg caagccatgg acgaacaggc cttgatgctc ttttgacagg
     3601 gagcgtcgcg tcgcgtattg cgggacgaag aattcgcccg ctgttgctgg tccgggcgag
     3661 gcagcctgct tgagcgtaaa atagaagaag aaaggcattg aaaggaaata gcacaactgg
     3721 aaataaaagc gacccgttgg aggtggaaag ggagcatcgt cccgcgactc gcagcgctt
//
LOCUS       DASZAK010000590         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_120757, whole genome shotgun sequence.
ACCESSION   DASZAK010000590 DASZAK010000000
VERSION     DASZAK010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120757"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..995
                     /locus_tag="VGT44_10375"
     CDS             252..995
                     /locus_tag="VGT44_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581243.1"
                     /translation="MQFVQRTMMGGSGKTVWVRRLEHTVLRIVSLAFSLASAHAIRWF
                     FAPLDGIDGLQPIITWVVALGFGLLGYFVSRGLAHRLMNHERIRAYAPICLIVEVVEI
                     FCNYALAAAVIQRASWLSAVPGAQRSFLTDATYVVLSIIPLVSLLLAVVDMDMERSKN
                     GTLSGGGFPSLTGNRFGKQAASPKVPTYTPSASAGTMSVPYPTPGTSSNGYAAGLPPM
                     NSSRGSASPFAPVGAAARPTSLLDSSGIP"
     gene            complement(881..1174)
                     /locus_tag="VGT44_10380"
     CDS             complement(881..1174)
                     /locus_tag="VGT44_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581244.1"
                     /translation="MPVGTPPDCALHQRAHLEMPPLVTISKLNGNTLIKRRPNMRGFL
                     FGESLTLAGGDDERAAYGIPLESRSEVGRAAAPTGAKGDALPLLEFIGGRPAA"
     gene            1271..2590
                     /locus_tag="VGT44_10385"
     CDS             1271..2590
                     /locus_tag="VGT44_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581245.1"
                     /translation="MSINYEYIKQLARETGARVTDLIPLAPQNDPFYTGTPNDWALGE
                     WFAHLWQAFGYTTKVHIRRVHYQIISQRPAVLLPNGKPYENTEECWGVLNLASKAARY
                     LRLVDPAAFDDRRNPDPLTYADYQVYEPGVSTSLRLWAGDLRLPAFPDLPDYYVRDFK
                     GTQRYHLEVWCEKSTMNDVLGPLCQAFRANLQTGLGELSITATLALVHRLQQAGKPAR
                     IFYVSDFDPAGQSMPVAVSRKIEYFVRTFGLDLDVRLFPVVLTQEQVASYQLPRTPIK
                     DTERRRVGFEDRYGQGAVELDALEALYPGELQQVLSAYMNPYYDYALDARVGEVAQQL
                     EEDLEALRQQVIARYTGNIQAVQAEYAQLKADFSGRMERYSQQLKGLWQAMRQELDLS
                     IPDLSSYAVPEPVIAEEIGEGLYNSERDYLEQIESYKQFQGKLVVAE"
     gene            2675..>3282
                     /locus_tag="VGT44_10390"
     CDS             2675..>3282
                     /locus_tag="VGT44_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581246.1"
                     /translation="MRRWLLACSTLLLGFFLLCWWWLQPVTPHVEAVLTLGRQVDTSY
                     SVVGSPTISAAFINQVLAASHSPAAGKGQALSDLGVKYGIDPVYALAFFWHESGFGTT
                     GEARVTRSLGNERCIADRPCIDQNRGGYALMHSWEDGFDHWYALIRYGYVEGQVTLPL
                     VGHVCTTLDQIVPVYAPSNDGNDVAAYIAAVKQAVETWRSGQV"
BASE COUNT          609 a          916 c         1084 g          673 t
ORIGIN      
        1 caggtcgccg atcaactgcg ccaggaattg ccccccaaga tgacgccgct tgccctcgaa
       61 gtgctgcgca tcaagggcaa aaacagcttt ttgacgctgg tgtgtcgcct ggcgcaagcg
      121 gcgtagcgct gtcctggttg aaattgggat gggtgagtga gtgaacaagc gcgtgtgtgg
      181 tcggtctggt ctggtcacgc acgcgcgtgg ctggcaggat ggaaccacct gcgcgcaaaa
      241 gggaggtggt tatgcaattt gtgcaacgca cgatgatggg tggctctggc aagacggtct
      301 gggtgcggcg tttggaacac acggtgctgc gcattgtctc gctggcgttc tcgctggcct
      361 cggcgcacgc tattcgctgg ttcttcgccc cgctggatgg catcgatggc ctgcaaccca
      421 tcatcacctg ggtagtggcg ctgggctttg gactgctcgg ctactttgtc agtcgcggtc
      481 tggcgcaccg gctgatgaac cacgagcgca tccgcgccta cgcgccgatc tgcctgattg
      541 tcgaggtcgt ggaaatcttt tgtaactatg cgctggcggc ggcggtcatt cagcgcgcct
      601 cgtggctcag cgccgtgccg ggggcgcagc gctcgttctt gacggatgcg acctacgtcg
      661 tgctgtcgat cattccgctc gtttcgctgc tgctggcggt tgtggatatg gatatggaac
      721 gctcgaaaaa cggcacgctc tccggtggcg ggttcccctc tttgaccggg aatcgcttcg
      781 ggaagcaggc cgcctcgccc aaggtgccga cgtatacgcc gagtgcgagc gcggggacga
      841 tgagcgttcc ctatccgacg ccaggcacga gcagcaacgg ttatgcggcg ggtctgccgc
      901 cgatgaattc aagcagaggg agcgcgtccc ctttcgcgcc ggtgggagcg gcggcgcgtc
      961 cgacctcgct cctggactcc agtgggatac cgtaagcggc gcgctcgtcg tcaccaccgg
     1021 caagggtcag cgactcacca aacaagaagc cgcgcatatt cgggcggcgc ttgatcaggg
     1081 tattcccgtt cagtttgctg atcgtcacca atggcggcat ttccaggtgc gcgcgctggt
     1141 gtaacgcaca atcaggcggc gtacctacag gcatggttga tctcctctcg atggtttcaa
     1201 ccatgccttt ttgctgtgga agttgtggtg cgtgagagaa gtgagtggca tgggaggatg
     1261 aaagaacagc atgtcgatta actatgagta tatcaaacaa ctggccaggg agaccggggc
     1321 gcgtgtgacc gacctcattc ccctggctcc gcaaaatgac cccttttata ccggcacgcc
     1381 gaatgattgg gccttgggcg aatggtttgc gcatctctgg caggcgttcg gctataccac
     1441 caaagtgcat atccggcgcg tgcattacca gattatcagc cagcgtccag cggtgctgtt
     1501 gcccaatggc aagccgtatg agaacacgga agagtgctgg ggggtgctga acctggcgtc
     1561 caaagcggcg cgctacttgc ggctggtgga cccggcagcc ttcgatgacc ggcgcaaccc
     1621 cgaccccttg acctatgccg attaccaggt ctacgaaccg ggagtctcga cctcgctgcg
     1681 cttgtgggcg ggggatttgc gtctgcctgc gtttcctgat ctgccggact actacgtgcg
     1741 cgacttcaaa gggacacagc gctaccacct ggaagtgtgg tgcgagaagt ccacgatgaa
     1801 cgatgtgctg gggccgctct gccaggcgtt tcgcgccaat ctgcaaaccg gcctgggcga
     1861 actgtccatc acggcaacgc tggcgctcgt gcatcgcttg cagcaggcag gcaaaccggc
     1921 gcgcatcttc tacgtctcgg acttcgaccc ggcggggcaa tcgatgccgg tggcggtctc
     1981 gcgcaagatc gagtacttcg tgcgcacgtt tggccttgac ctggatgtgc ggctctttcc
     2041 agtggtgctg acgcaggaac aggtggcgtc ctaccagttg ccgcgcacgc ccatcaagga
     2101 caccgaacgg cggcgggttg gctttgaaga tcgctacggt cagggggcgg tggagctaga
     2161 cgcattggaa gcgctctatc cgggggaact gcaacaggtc ttgagcgcct acatgaaccc
     2221 gtattatgac tatgcgcttg atgcgcgggt gggggaagtc gcgcagcaac tggaagagga
     2281 cctggaagcg cttcggcagc aggtgattgc gcgctacacc ggaaatatcc aggccgtgca
     2341 agcggagtat gcgcaactca aggccgattt ctccgggcgc atggaacgct atagccagca
     2401 actcaaaggc ttatggcaag ccatgcggca ggaacttgac ctgtccattc cagacctgtc
     2461 ctcttacgcc gtgccagaac cggtgatcgc cgaagagatt ggggagggtc tctacaacag
     2521 cgagcgcgac tacctggaac aaatcgaatc ctacaagcag ttccaaggca agctggtggt
     2581 cgcggaatag cgcagaaatg aggaacctgg ggcgtcgcgt tgacacagaa ccggcgcggc
     2641 gtccctggca ggtggatgga agggaaggtg tggtatgaga cggtggttgc tcgcgtgttc
     2701 aacgctgttg ttagggttct ttctgctgtg ctggtggtgg ctgcaaccgg taacgcctca
     2761 tgtcgaggcc gtgctgacgc tgggcaggca agtggatacg tcgtactcgg tggttgggtc
     2821 tccaacgatt tcggcggctt tcatcaacca ggtactggcg gcgtctcact cgcctgcggc
     2881 gggcaaggga caggcgctct ctgacctggg ggtcaagtac ggcatcgacc cggtgtacgc
     2941 actggccttc ttctggcatg aatcgggctt cggcacaacg ggcgaggcgc gggtgacgcg
     3001 ctcgctgggc aatgaacgct gcatcgcaga tcgtccctgc atcgaccaga accggggcgg
     3061 ctatgcgctg atgcacagtt gggaagatgg ctttgaccac tggtatgcgc tcatccggta
     3121 cggctatgtc gagggacagg tgacgcttcc cctggttggg catgtctgta caacgcttga
     3181 ccagatcgtg ccggtctatg ctcccagcaa tgacgggaac gatgtcgcgg cctatatcgc
     3241 ggcggtcaaa caggcggttg agacgtggcg tagtgggcaa gt
//
LOCUS       DASZAK010000591         5712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_49276, whole genome shotgun sequence.
ACCESSION   DASZAK010000591 DASZAK010000000
VERSION     DASZAK010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49276"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..1545)
                     /locus_tag="VGT44_10395"
     CDS             complement(109..1545)
                     /locus_tag="VGT44_10395"
                     /inference="COORDINATES: protein motif:HMM:NF039478.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6519 domain-containing protein"
                     /protein_id="HEV2581247.1"
                     /translation="MDGNFKTDFSNDKFDRAKRFSRVLMQQGRVQLDADWNEQIDILL
                     YYVRTLVIDLAGPFWGPGDGFKIAPPPGGDMSLFSVTQGHYYVDGILSENDADQPYYL
                     PATSDTSSTASGTTSATKGSRRQRRSTKASTSAFLVYLDVWERQVTTLEDVSILEVAL
                     KGPDTTTRAKVMWQVRTWDKLPEGTPTTGTDVKPYVLKYWPQWLQQWFPPNRGYLKAG
                     INQDNGVSRDPCITAPTSQYRGIENQLYRVEIHDGGSVSSGSRGGSTTGGPTFKWSRE
                     NGSVVLPISDLKGNIVTVKNLGRDDRFGLRKNDWVEIVDDSTVLDSPNPLLQVETVDY
                     TNSQVILKTAPSSNIGDNPLLRRWDQHAGDSDDPHAITLNNDGVAILQEGAWLTLEDG
                     VQIWFQSGASTTDTPTYRSGDYWLIPARTALGNIIWPQDDKGPVALPPHGVQHHYAPL
                     AVIVPDANGNVTESSITDVRDIINKLTI"
     gene            complement(1565..3937)
                     /locus_tag="VGT44_10400"
     CDS             complement(1565..3937)
                     /locus_tag="VGT44_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581248.1"
                     /translation="MSDRLDRLYTLLPRYYRQRDADERWPLQALLHVIAEQVNVVEDD
                     IAQLYENWFIETCQDWVVPYIGDLIGYEIVHDAGEPGDITTLEGKQRNKIMIPRREVA
                     NTIRYRRRRGSLALLDQLAHDVAGWYARAVEFYLFLAQTQSLNQLRLDRGRLVSVRDD
                     NNLHRVNSPFGSLAHMVDVRRIDSSHTPGRYNIPEIGLFVWRLKVYPVTRAPAYCVER
                     VAPECYTFSVLGNDSPLYTNPEPQVDPTRIPEALNLPMPIGRRAFKNHKTSYYGESKS
                     MQIWIGVRQHKDDGQGGTGQSEHAFQHQASEGSHRHQHHGEHGSDHSDEWVVVKQPVP
                     AEHIVPANLSDWHYRPHHGEVAVDPELGRIVFPEDELPVGVWVSYYYGFSADISGGEY
                     NRPLSQPAEYSLYRVGEGEQYETLQAALDAWDAAKEALEQSEIEEPGETASQERHEER
                     KERAAHPELLHAIIEIADNRVYTEELDIELEAYESLQIRAANYARPVIHLLDRETNLP
                     DSWYIAGDTGSRFSLDGLLLTGRGVKAEGKLASLCIRHCTLVPGWEIHDDCQPGHPAA
                     PGLELSDMSTLVTIEHSILGPIHVRQNEARMDPLTMRISDSILDATGVKQEALTGPGC
                     PVAPAVLTIERSTVFGQVEVHAIDLAENTIFNGLVKVARRQEGCMRFCYAPYGSRTPH
                     RYNCQPDLVVQLVDEDEAGETLPDTDKAQMEQSERERVRPLFNSTRYGSPTYCQLSNN
                     CALEISHGADDEAELGVYHDLFQPQREANLRTRLNEYIPAGMDASIVFAS"
     gene            complement(3934..>5712)
                     /locus_tag="VGT44_10405"
     CDS             complement(3934..>5712)
                     /locus_tag="VGT44_10405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEV2581249.1"
                     /translation="LDQQWLFDQDVSLATLRQVTVYAQSDEQGLTDEPIDPVLEGVCG
                     DTVELDDVYAGLKTGRWAIVTGERTDIFGVIGVTGTELVMLAGVTQGVHQVSVPTPTR
                     SGSGGGGTSQGNTQLVNLPGDKTHTFLQFATPLAYSYKRDTVTIYGNVAKATHGETRN
                     EVLGSGDGSKALQSFSLKQSPLTYLTAPTPAGAASTLMVYVNDIQWHETESLVEEGPN
                     DRIYTTKTDDNDKTGTIFGNGVHGARLPTGAENVKATYRSGIGKPGNVKANQISMLAT
                     RPLGVRAVINPLAASGGADRDSRDQARRNVPVAMLALDRLVSVQDYADFARTYAGIGK
                     ASALLLSDGQRQLVHLTIVGADNIPIDKTSDLYLNLFAALRQFGDPQVPLQVDLCEVK
                     LLVISASIEILPNSLWEVVALNIRTALLDAFSFDNRALGQPVFQSEVISIMQNIAGVV
                     YVHIEIMDAVDQDILRNALNKIGNERADSGTGNTQGGGTGSGGGGTDTTADELANDLG
                     LTLKPRIDVSLAGPFYYTVKQGDNLSSIAKFFGISVVELQKLNPQLKDPLLVGTLVLI
                     QRIRPAQLAFLTPDVPDTLILREKTA"
BASE COUNT         1185 a         1657 c         1550 g         1320 t
ORIGIN      
        1 gctttccagt ataggtaccc atactattcg tcaccggatg cgccatagca gcccatgtgt
       61 cgacctgcga cgaaacagtg acgatctgcc agtgatgctt tctgatcatt atattgtcag
      121 cttattgatg atgtcacgta catcggtgat gctactttcc gtcacatttc cgttcgcatc
      181 aggcacgatc acagcgagtg gcgcgtagtg atgttgcact ccatgtggcg gcaacgctac
      241 gggtcccttg tcgtcctggg gccagataat attgccgaga gcggtacgag cggggatcag
      301 ccagtagtcg ccgctgcgat aggttggggt gtccgtcgtg gatgcaccgg actgaaacca
      361 gatttgcacc ccgtcctcta atgtgagcca agcaccttct tgcagaatcg ccacaccatc
      421 gttgttcagt gtgatggcat gggggtcatc cgaatcgccc gcatgttgat cccagcgccg
      481 caataatggg ttatctccta tgttagagga aggtgccgtt tttaggatga cctgactatt
      541 ggtatagtca acggtctcga cctgcagcag tggattaggg ctgtccagca cagtgctgtc
      601 atcgacaatt tctacccaat cattcttcct gaggccgaaa cggtcgtcac gccccagatt
      661 cttcaccgtg actatattcc ccttcagatc gctaatgggt aagacaacgg agccattctc
      721 ccgtgaccat ttgaatgtcg gaccgccagt agtgctgccc cctctgctcc ctgaggacac
      781 tgagccgcca tcgtgaatct ccactctgta gagctgattt tcgataccac gatattgtga
      841 ggtaggcgcg gtaatgcaag gatccctact cacaccattg tcctggttaa tcccggcttt
      901 gaggtaccca cggttcggcg ggaaccactg ttgcaaccat tgaggccaat acttcagcac
      961 ataaggtttg acgtctgtac cagtagtagg cgtgccctct ggaagcttgt cccaggttct
     1021 cacctgccac attactttgg cccgcgtcgt tgtatccggt cccttcaaag caacctcaag
     1081 aatgctcaca tcttcgagcg tcgtcacctg gcgctcccag acatcgagat agacgaggaa
     1141 agctgaggta cttgcctttg tacttctgcg ttgtcgtcta ctcccttttg tagcggatgt
     1201 agtgccggac gcggttgagg aggtgtcgga tgttgccgga aggtaatagg gctggtccgc
     1261 gtcgttctca gagaggatac catcaacgta ataatggccc tgtgtaacag agaagagaga
     1321 catatcacca ccagggggcg gcgcaatttt gaaaccatct cccggccccc agaacggccc
     1381 ggcgaggtct atcaccagcg tgcgcacata ataaagcaga atgtcaatct gttcgttcca
     1441 gtcggcatca agctgtaccc gtccttgctg catcagcacg cgggagaagc gtttggcacg
     1501 atcaaatttg tcattagaaa aatcggtctt aaagttgccg tccatgtgtt ctttatcctc
     1561 caagctaact ggcaaagacg atgctggcat ccatccctgc aggtatatat tcgttcaggc
     1621 gagtacgtag attcgcttct cgctgcggtt gaaacaggtc gtggtagact cccagctctg
     1681 cttcgtcatc cgcgccatgg gagatctcca gggcacagtt gttggagagc tggcagtagg
     1741 taggagagcc atagcgtgta ctgttaaaca aggggcgcac acgctcgcgc tcgctctgct
     1801 ccatttgcgc cttatcggta tcgggaagtg tctcgcccgc ttcgtcttca tccacgagct
     1861 gcaccaccag gtccggctga cagttgtagc ggtgcggtgt gcgcgatcca tatggggcat
     1921 agcaaaagcg catacagccc tcctgtcgac gtgcaacctt gacgaggccg ttaaagatgg
     1981 tgttctctgc taggtcgata gcatgtacct ctacctgccc aaacaccgta ctgcgttcga
     2041 tggtcaacac ggcgggagcc acggggcaac ccggccccgt cagcgcctct tgtttgacac
     2101 cggtcgcatc gagaatactg tcgctgatac gcatggtgag cgggtccatg cgtgcctcgt
     2161 tctgcctcac atgaatcgga cccagaatgc tgtgttcaat agtgaccagt gttgacatat
     2221 cggacaattc caggcctggt gcggcaggat ggcccggctg acaatcatca tgaatctccc
     2281 agccgggcac cagcgtgcaa tgacggatgc agagcgaggc cagtttccct tccgccttca
     2341 cgcctcttcc ggtcaggagc agaccatcca gggagaaacg gctaccggta tcgcctgcta
     2401 tataccagga gtctggcaga ttcgtctccc ggtcgagcag gtgtatgaca gggcgggcat
     2461 agttggcggc ccgaatttgc aggctctcat atgcttcgag ttcgatatcg agctcttcgg
     2521 tatacaccct gttgtcggca atctcgatga ttgcatgtag gagttcgggg tgcgcggccc
     2581 gttccttgcg ctcttcgtgg cgctcctgcg aagcagtctc acccggctct tctatttcgc
     2641 tctgctccag cgcctccttt gcggcatccc aggcatccaa tgcagcctga agcgtctcgt
     2701 actgctctcc ctcacctacg cggtagaggc tatactcggc aggctgcgag agcggtcggt
     2761 tatattcgcc gccgctaatg tcggcactga agccatagta ataggagacc cagacgccaa
     2821 ctggcaattc atcctctgga aagacgatgc gccccaattc tggatcgact gccacctccc
     2881 catgatgggg ccggtaatgc caatcgctca aattggctgg cacgatgtgc tccgctggca
     2941 caggctgctt caccacaacc cactcgtcgg agtgatctga gccatgctca ccgtgatgct
     3001 ggtgacgatg cgacccctcc gaagcttggt gctgaaaggc atgttctgat tgacccgtac
     3061 ccccctgtcc atcatctttg tgctggcgga cgccaatcca gatttgcata ctcttgctct
     3121 cgccgtagta actggtctta tggtttttga aggcacgtcg cccaatcggc ataggcaagt
     3181 tcaacgcctc tggtatccgc gtgggatcca cctgcggctc cgggtttgta tagagtgggc
     3241 tatcgttacc gagcacgctg aaggtataac attcaggagc aacgcgctcc acacagtagg
     3301 cgggggcacg cgtcaccggg tagactttca gacgccagac gaagaggcca atctcgggga
     3361 tattgtagcg tccgggggta tgcgacgagt cgatgcgcct cacatcgacc atatgagcca
     3421 gcgagccaaa cggactattc acgcgatgga ggttgttatc atcacgcaca ctgaccagcc
     3481 tgccccgatc aagtcgcaat tggtttaacg attgggtttg cgcaaggaat aggtaaaact
     3541 ccacggcgcg tgcgtaccaa ccagcgacat cgtgggcgag ctgatcgagc agggccagcg
     3601 agcccctgcg ccgtcgatag cgaatcgtgt tggcgacctc gcggcgtggg atcatgattt
     3661 tattgcgctg cttgccctca agcgtcgtga tatcgcctgg ctcacccgcg tcatgcacaa
     3721 tttcgtagcc gatcaagtcg ccaatatagg gcaccaccca atcctggcag gtctcgataa
     3781 accagttctc gtacagctgg gcgatatcat cctcaacgac attcacctgc tcagcgatca
     3841 catgcaggag cgcctgcagc ggccagcgct cgtcggcgtc ccgctgacga tagtaacgtg
     3901 gcaataaggt atataatcga tcaagtcggt cactcatgcc gttttctccc tcagaataag
     3961 cgtatcgggg acatcgggtg ttagaaatgc aagctgagcc gggcgaatcc gctggatgag
     4021 cacgagcgtt cccaccagaa gtggatcctt tagttgcggg tttaacttct gcagttcgac
     4081 cacactaata ccaaagaatt tggcaatact actcaaatta tcgccctgct ttacagtgta
     4141 atagaacggc cctgccaagc tcacatcgat gcgtggtttc aacgttaagc cgagatcatt
     4201 ggcaagttca tccgccgtgg tatccgtgcc accaccaccc gaaccggttc cgccgccctg
     4261 agtatttcct gtgcctgagt cagcccgctc attgccaatc ttattgagcg cattacgcaa
     4321 tatatcctgg tctacggcgt ccataatttc gatgtgaaca tagaccacac ccgcaatatt
     4381 ttgcatgatg ctgatcacct cgctttgaaa gactggttgc cccagtgctc ggttatcaaa
     4441 actgaaggcg tcgagcaagg cggtgcggat gttcagtgcc accacttccc atagggaatt
     4501 cggcagtatc tcgatgcttg cactgatgac caggagcttc acctcgcaaa gatcgacctg
     4561 caacggcacc tgggggtcgc cgaactggcg cagcgctgcg aacaggttca agtacaggtc
     4621 tgaggtctta tcaatcggga tgttgtccgc gccaacgatg gtcagatgca caagctggcg
     4681 ctgcccatcc gagagcagca aggcactggc tttgccaatc ccggcatagg tgcgggcgaa
     4741 gtcggcgtag tcctgcaccg agaccaagcg atccagggcc agcatggcaa ccggcacatt
     4801 gcgccgcgcc tggtcgcgag agtcgcggtc cgcgccgccg gatgcggcca gcgggttgat
     4861 gacagccctc acccctaatg ggcgcgtggc gagcatactg atctgattcg ccttcacatt
     4921 gcccggcttg ccgatgccac tgcgataggt tgctttgaca ttctcagccc ccgttggcag
     4981 gcgagcgcca tgcacgccgt tgccgaaaat ggtccctgtt ttgtcattat cgtcggtctt
     5041 ggtggtgtag atgcggtcgt tcggcccctc ctcgaccagg ctctccgttt cgtgccactg
     5101 gatatcgttg acatacacca tgagcgtgct cgccgctcca gcaggtgtag gcgcggtcag
     5161 ataggtcaag ggcgactgtt tcagggagaa actctgcagc gccttgctgc catcaccact
     5221 gcccagcact tcgttgcgcg tctcgccgtg tgtcgccttc gctacgttgc cgtagatggt
     5281 gacggtatcc cgcttatagc tatatgcgag cggcgtggca aactgcagga aagtgtgcgt
     5341 cttatcgcca ggcaggttta ccaactgtgt attgccctgg gatgttccgc cgccaccact
     5401 tcctgacctc gttggtgtgg gaacacttac ctggtgtacc ccctgtgtca cgcccgcgag
     5461 catgaccagt tccgtgccag taacgccgat cacgccgaag atgtcggtac gctcgcccgt
     5521 gacgatggcc caacgcccgg tcttcaagcc tgcatagaca tcgtccaact caacggtatc
     5581 accgcatacc ccctccagca ctggatcgat cggctcatcg gtcagaccct gctcatcgct
     5641 ctgggcatag acagtgacct ggcgcagcgt tgccagggag acatcttgat cgaatagcca
     5701 ttgttggtcc ag
//
LOCUS       DASZAK010000592         8726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_547080, whole genome shotgun sequence.
ACCESSION   DASZAK010000592 DASZAK010000000
VERSION     DASZAK010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547080"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="VGT44_10410"
     CDS             <1..832
                     /locus_tag="VGT44_10410"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2581250.1"
                     /translation="QQQQPAGWGAPATQPPQGGWNNQGSGQQPWDQQQVATPPPQDPW
                     SQQQQQPIPAWGGQNNQQQAPNAFGASGSNQGWGAGSAGQVGQADPWGQQAQQPQQAQ
                     PGSWPGMAGAGQQQPVSPPPGSNQAQQPPSWQQGGFNQGQMGQPAQQGYGGFDTPDGD
                     RTMLGPVGARGPAAQGRTGIVRIEEGKEPGRIYEMRKETLSIGRSRESDIFLEDLAVS
                     RLHASIISMGNSNYALRDEGSANGTKVNGQTVNKYQPYTLQEGDKIQLGQTVLVFARR
                     "
     gene            1099..2226
                     /locus_tag="VGT44_10415"
     CDS             1099..2226
                     /locus_tag="VGT44_10415"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2581251.1"
                     /translation="MLQDPMIGTALGNYRILAPLGQGGMAHVYRARQENLDREVAVKV
                     LPPWYASDRSFVERFKQEARLVARLSHPNIVTIHDYSESNGHLYIVMELVDGGTLKQV
                     LDRQRTPNNAGSNGTMQVIEANRIFQQLSSALTYAHEKGIIHRDIKPVNVLMDLSRRP
                     ILSDFGIAKVLASGNGLTRPGAGVGTPEYMSPEQCRGEEVDGRADIYALGVMLFEALA
                     GRTPFVGDNYPALAYNHLHTQPPDPRAFNPQLSPQICRVILTALEKKPERRFQRASDM
                     ALALNEAVHPSSIGPISPMPMTPPNAIDQAPTVKPNSIDRVPTTPVYACPKCQRPNKP
                     DTFFCVGCGALLNQCPRCRNQNRAKDRFCTRCGQPLPYRLQ"
     gene            complement(2232..2729)
                     /locus_tag="VGT44_10420"
     CDS             complement(2232..2729)
                     /locus_tag="VGT44_10420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2581252.1"
                     /translation="MNMARSTTTSLSPNRILVVEDDFDIRQVLSVYLKHSGFNVSSAS
                     DGQEAIALIVSFKPELVVLDLMMRPVNGWEVLQWLRAQRMTPPLPVLVLTALSRLDLQ
                     VQGYEEGAIDYIIKPTQPGVIVDRIHALLRLSAEQRTLVQRKRIDEQRSMLERVQAQP
                     DEFVL"
     gene            complement(2779..4878)
                     /locus_tag="VGT44_10425"
     CDS             complement(2779..4878)
                     /locus_tag="VGT44_10425"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2581253.1"
                     /translation="MLESSATQQVEYFLRQHEDRLRSALQYATQANSGPEKGSDAWLL
                     LTALISDDRSELDASFEQSGRALAQHGGKLEARILGLQTYVDALAREFRGLFDDHEQG
                     QMYLLEANTRLFQLHSLSLMALTRGYQSVVDQVFLARQRMERLLEKRLQVLQRINSIS
                     NSAIDLDQALESITRQVADELRTELCSVFFYDEMQRVLTLRATNEPRPMGGMHYTLRL
                     GEGYSGWVAEKGSPVLTRDALGNPAFASEARAYGQEYSGLMSLPIIFFGNVERLIGVI
                     NVQTRDPRDFSGEEMNFVEVVAGMIAINVENGRLYEQTDEQLRRKVHELATLHRVSSI
                     IASTLDLDRVLQIITTQAVQLSGADRSCIFELDEDRRCLGVIAHYGLNSADVQGIQVP
                     VGHCCAGRAVSTGRPSMAVDCFHSDEQCFMRHDPLASADMHSVLCVPLQVKQKVLGCI
                     CIYSSHRHMLSQEQMQLVITFANEAAIAIDNARLFEEARRGLEIKTTLLRELHHRVKN
                     NLATVAGILSLQRRRTRSPEVRYILAESVNRVQGLAATHDLLSHEDVSEARVDDIARK
                     IVGVANANLRPPETHIVFEVTPCPVVIPSRAVEILALVLNEMVSNAIKHGIDGQPRGA
                     VTIRGREEHGEVVVEVLDTGKGPASLPEEESSEGLGLSLIKNLIGDLGGSYVLRRTEE
                     MPPRTCAEVRFPLKRRL"
     gene            5151..5921
                     /locus_tag="VGT44_10430"
     CDS             5151..5921
                     /locus_tag="VGT44_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581254.1"
                     /translation="MKCASCGMPLSPNRPQANCPKCGAPVNAHAAFSPSVQSPSSPQP
                     MPGSGWQTGMRERAGFAPAAPGQPRWQPSGPSQPQDQNRSTRLGFLIAGLCILAGAFL
                     LILVYFLAVGGPGGQTAGTQTTPQATVIPSPSPTSAPSPTATPFPGQQYIDNAQLSSS
                     PPPTVQTTSTFTSNQKIYVTFDLHPNGQKGVVCFVWYLNGQQAATYNFPVNGSARSSY
                     AYATLGGTGTGYVDLFWANDTTCSNELLAQQVPFTLTK"
     gene            complement(5987..8611)
                     /locus_tag="VGT44_10435"
     CDS             complement(5987..8611)
                     /locus_tag="VGT44_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2581255.1"
                     /translation="MSLDIELYRRTLMMPFGRQGQQLRRISVIDLQPEDAELTLVMVH
                     GYGGSATQWQYQLQSFGQTMRVVAYDLRGHGFSDDPADLPCTMESLVDDLEAVIERLN
                     VPQPFYLLAHSFGGAIATEYALRHPEHVRGLVLIGVPSRFIVRDIVRRLMLVPDPIFS
                     GVAKLIKVALYAPQRTLKRMLNNALSTWKGPASLERLRVPALVMMGHRDTVFLREHYE
                     DVARSIPGAQQVVIPVSAHLVQLERPDAVNRAIRRFIDTRQKTIAAPGSSTVSAASAH
                     ALRHVEMPWLQHYDNDVPETIPRSKMLVTDLLNNAAIAYASRSALIFFGQHISFRELD
                     RSSNRFAHALRELGVNKGDRIAILLPNVPQFVVGVYGTLKAGGVVVLGSPLSNEEEIL
                     FQLQHSGAKMLLALSSYAPMVERACQQADIRQVIYTDVREYLPLRQRVALANMIDGAG
                     ASKAALAPPASPATPGEDGTAAAHVEQDSVPVERVAPGGHTWTQYAFQRLLHGQPARL
                     IDGGATSDDLAVLLYTSGTTDTPKGVMLSHGNLVINVAQQRHWLSDSKRGREVTLCVL
                     PLTHSYGFTSCMNLSIALAATQVLLPTQRLDQVMESIKQSHPTILPGVPALYLSIANY
                     PKVRSYGVAAIRTCVSGSAPLPVEVQEAFEKLTRGRLVEGYGLTEASPSTHCNLLKGE
                     RRVGSIGLPLPGTSARIVDVKTGEALPPGQVGELLVRGPQVMQGYWNMPDETRATLQG
                     GWLHTGDLASMDEDGFFTIVDRKKDLILAGSYNVYPRDVEEVLYEHPKVLEVAVVNMR
                     HALASSENNTAGGEDTSPFIKAVVVLKRGEKATAEELLALCRERLDAYKVPGQIEFRT
                     ELPKNFVGKVLRRLLVEA"
BASE COUNT         1834 a         2721 c         2516 g         1655 t
ORIGIN      
        1 tcagcaacag cagcccgccg gctggggcgc gcctgctaca cagcctccgc agggtgggtg
       61 gaataaccag gggagcggcc agcagccctg ggatcagcag caggtagcga cgcctcctcc
      121 tcaggaccca tggagccagc agcagcaaca gcccatcccg gcctggggcg ggcaaaacaa
      181 ccagcagcag gctccgaacg ccttcggcgc gagcggatca aaccagggct ggggcgcagg
      241 atcggcgggc caggtgggac aggccgaccc ctgggggcaa caggcacagc agccacagca
      301 ggcacagccc ggcagttggc ccggtatggc gggagcaggc cagcagcagc cagtatcacc
      361 gcctcccggc agcaatcagg cgcagcagcc cccatcctgg caacagggag gcttcaacca
      421 gggacaaatg ggacaaccgg ctcaacaggg ctacggcggt ttcgatacgc cggacggtga
      481 ccgcaccatg cttggtccgg tcggagcgcg tgggcctgcc gcgcaaggcc gcaccgggat
      541 tgtgcgtatc gaggagggta aggaaccagg acgtatctac gagatgcgta aagaaacgct
      601 gagcattggg cgcagccgcg aaagcgacat cttccttgag gacctggccg tctcacgcct
      661 gcatgcttcc atcatcagta tgggcaacag caactacgcg ctacgcgatg agggaagcgc
      721 caacggtaca aaggtcaacg gacagacggt caacaagtac cagccctata ccttgcaaga
      781 aggtgataaa attcaattgg ggcagacggt actggtcttc gcccgccgct aaacacggta
      841 cactgtaggg ttgcaatgat gcgctttacg gaatgattcg gtctctccga agtagttcgt
      901 caaagttgat ttggccagta ggggcgagcc ttgatggtcg cccgcccgcg cctgtcccga
      961 tgcgtgttac tactacgagc gggcgaccat caaggctcgc ccctactggc caaatgaaga
     1021 atgacagaca aatcggcaac cacggagaga ttcgtaccgt cccagggatg agagacgcga
     1081 ggagcaacaa atcggcttat gctgcaagat ccgatgatcg gcacggcgct ggggaattac
     1141 cgcatccttg ctccacttgg tcaaggggga atggcgcatg tctaccgggc gcgccaggaa
     1201 aatcttgatc gcgaggtcgc ggtcaaggtg ctgccgccct ggtatgcaag cgaccgcagc
     1261 tttgtggaac gtttcaagca agaggcgcgc ctggtcgcgc ggctctctca tcctaatatt
     1321 gtcactatac acgattacag cgagagcaat ggacacctgt acattgtcat ggagctggtc
     1381 gatggtggca cactcaaaca ggttctcgac cggcagcgca ccccaaacaa cgcgggatcg
     1441 aacggcacaa tgcaagtgat agaggccaat cgtattttcc agcagctcag cagcgccctg
     1501 acctacgcac acgaaaaagg catcattcat cgtgatatca agccggtcaa cgtcctgatg
     1561 gatttgtcca ggcggccaat cctatccgac ttcggcatcg cgaaggtgct ggcttctggt
     1621 aatggcctta cccgtcccgg agcaggggtt ggcaccccag aatatatgtc gcccgaacaa
     1681 tgccggggag aagaggtcga tgggcgggca gatatctatg cgctcggcgt tatgctcttc
     1741 gaggcgctgg ccgggcgcac acccttcgtc ggggataatt accctgccct ggcttacaac
     1801 cacctgcaca cacagccacc tgacccgcgc gcgttcaatc cgcaactcag tccacagatc
     1861 tgccgcgtga tcctgacggc gctcgaaaag aagccggagc gacgattcca gcgcgccagc
     1921 gatatggcgc tggcgctcaa cgaagctgtc catccctcca gcatcggccc aatctcaccg
     1981 atgccaatga caccgcccaa cgcgattgac caggcgccta ctgtcaagcc aaacagcatc
     2041 gaccgtgtcc ccacaacgcc ggtctacgcc tgcccgaaat gccagcgacc caacaagccg
     2101 gatacctttt tctgcgtagg ctgcggtgcg ctgctcaatc agtgccctcg ctgccgcaat
     2161 caaaaccggg ccaaagatcg cttttgcacg cgctgtggcc agcctctgcc ttatcgcttg
     2221 cagtaaccct cctacaagac gaactcgtcg ggctgcgcct gcacgcgctc tagcatagat
     2281 cgctgttcgt caatgcgttt gcgctggacc agggtgcgtt gttccgcact caagcgtagc
     2341 aaggcatgaa tgcggtcaac aatcacacca ggttgggtcg gcttgataat atagtcgatc
     2401 gcgccctctt catagccttg cacctgtagg tccaggcgcg aaagcgcagt cagcaccagc
     2461 accggcaggg ggggtgtcat gcgctgcgcg cgtagccatt gcagcacttc ccatccattc
     2521 accggtcgca tcatcagatc gagtacgacc agttcgggct tgaagctgac gatcagcgca
     2581 atcgcttcct ggccatcgct tgcgctgcta acattaaaac cggaatgctt caaatacact
     2641 gagaggacct gccggatgtc aaaatcatcc tctacgacca ggatgcggtt cggggatagc
     2701 gaagttgtag tacttcgagc catgttcatg ccgatcacct ccactcccac atagggacct
     2761 atacgatagt aaaccaggct agagccgccg tttcagcggg aaccgcactt cggcgcacgt
     2821 gcgcggaggc atttcctcag tgcggcgcaa gacatacgat ccaccgagat cgccgatcag
     2881 attcttgatg agtgaaagac ccaggccctc gctgctctcc tcctccggca gactggccgg
     2941 gcctttgccg gtatccagca cctcgacaac gacctcgcca tgctcctcgc gaccgcgaat
     3001 agtcaccgcg ccgcgcggct ggccgtcgat gccatgcttg atcgcgttcg agaccatctc
     3061 attcaacacc aacgccagga tttccaccgc gcgggaggga atgaccaccg ggcaaggcgt
     3121 cacctcaaag acgatgtgcg tctctggcgg gcgaaggttg gcgttggcca cgcccacaat
     3181 tttccgcgcg atatcgtcca cacgcgcctc gctgacatct tcgtgcgaga gcaggtcgtg
     3241 cgtggccgcc agcccctgca cgcggttgac gctttcggcc aggatgtagc gcacctccgg
     3301 cgagcgggtg cgccgccgtt gcagtgaaag gataccggcc actgttgcaa ggttgttttt
     3361 gacgcgatgg tgcaattcgc gcagcagggt tgttttgatc tccaggccac ggcgcgcctc
     3421 ttcaaacaga cgcgcgttat cgatggcaat cgctgcttcg ttggcgaacg taatcacgag
     3481 ctgcatctgc tcctggctga gcatatggcg atggctgcta tagatgcaga tgcatcccag
     3541 caccttctgc ttgacttgca agggaacaca caataccgaa tgcatgtccg cggatgccag
     3601 tggatcgtgg cgcatgaagc actgctcatc gctgtggaaa caatcgacgg ccatgctcgg
     3661 ccgcccggtt gaaacggcgc gcccggcgca gcagtggcca accggcacct ggatgccctg
     3721 cacatctgcg ctgttgaggc cgtagtgcgc gatgacaccc aggcagcgcc ggtcttcgtc
     3781 caattcaaag atgcaggaac gatccgctcc gctcaactgc accgcctggg tcgtgatgat
     3841 ttgcaagacg cggtccagat ccagcgtcga ggcaataatc gaagaaacac ggtgcagcgt
     3901 ggccagttca tgcactttgc ggcgaagttg ctcgtcggtt tgctcgtaca accgcccatt
     3961 ttcgacattg atggcgatca tgccggccac cacctcgaca aagttcatct cttccccgct
     4021 aaaatctcgc ggatcgcggg tctgcacatt gatgaccccg atcaatcgct cgacattgcc
     4081 gaagaagatg atgggtaagg acatcagccc gctgtattcc tgtccatacg cgcgcgcctc
     4141 gctcgcaaag gcaggattgc ccagcgcgtc gcgcgtgagt accggtgagc ctttttcggc
     4201 cacccaccca ctatatcctt cgcccagtct caaggtatag tgcatcccgc ccatcggacg
     4261 cggctcgttg gtcgcgcgca gcgtcaaaac acgctgcatt tcatcgtaaa agaaaaccga
     4321 acagagttct gtgcgcagtt catccgcgac ttgcctggtg atcgactcta acgcctggtc
     4381 gaggtcgatg gcagaattgc tgatgctgtt gatgcgctgc aagacctgca agcgtttctc
     4441 gagcagccgc tccatgcgct gccgcgccag gaacacctgg tcgaccaccg actggtagcc
     4501 gcgcgtcaag gccattaatg aaagcgaatg tagttggaac aggcgagtgt tggcttcgag
     4561 caaatacatc tggccctgct cgtgatcgtc gaataggcca cgaaattcgc gggccagggc
     4621 atcgacgtag gtttgcaaac cgagaatgcg cgcctcaagc ttgcccccat gctgggccag
     4681 ggcgcgacct gattgctcaa agctcgcatc gagttcgctg cgatcatcgc ttatcagcgc
     4741 agtgagcaat aaccaggcat cgctgccctt ttctggcccg gagttcgcct gcgtcgcgta
     4801 ttgcaatgca gagcgcagcc tgtcttcgtg ttgtctgagg aaatattcaa cctgctgtgt
     4861 ggcgctgctc tctagcacat cttttccctt ttcgagatgg caggggaata ctcgtgtggt
     4921 tgcccttcca ccacctcagc ttacaagcat attacacttg cttcgcttgt gcatattgta
     4981 aaggtggaat gctttcctgt caagaagcgc tcaggcatgg cgaccgcaag ggtgcgccat
     5041 gatacattct gatgaagaat agcccattta gcagcagttc gcttactcga ctccactcag
     5101 tacgttaaat gagtaataac agcgaagtta agcgcaggaa ggatatcgac atgaaatgtg
     5161 catcgtgcgg catgccgctt tcgccaaatc gcccacaggc gaactgtccg aagtgtggcg
     5221 cgcctgtaaa cgcacatgct gcgttcagtc cttccgtcca gtcgccatct tctccccagc
     5281 cgatgcccgg atcggggtgg caaacgggga tgcgcgagcg agcgggcttc gcccccgccg
     5341 cgccaggaca gccgcgctgg cagccatcag ggccgtctca gccgcaggat caaaatcgca
     5401 gcactcgtct cggttttctc attgccgggc tgtgcatcct ggcgggcgcg ttcctgctga
     5461 tcctggtcta tttcctggcc gtcggcggac cgggcggtca gactgccggt acacagacaa
     5521 caccccaggc gactgtcatc ccatcgccgt cgcccaccag cgccccctcg cccaccgcga
     5581 ctcccttccc aggacagcag tatatcgaca acgcgcagtt gagcagcagc ccgccgccaa
     5641 cggttcagac tacgagtacc tttacctcga accagaaaat ctacgtgacc ttcgacctgc
     5701 accccaacgg gcaaaaaggc gtggtatgct ttgtctggta tttgaatgga caacaggccg
     5761 caacttataa cttcccggtg aacggcagcg ccagatcaag ctacgcttac gccaccttag
     5821 gcggcactgg caccggctat gtagacctct tctgggccaa cgacaccacg tgcagcaatg
     5881 aactgctcgc ccaacaagtc cccttcaccc tgacaaagta accccttgac ctccataccc
     5941 aagcgtcccc acacctcccc acgtcctccc ccgcccctac ggacgattac gcttctacga
     6001 gcagtcgcct caacaccttc ccgacaaaat tcttggggag ttcggtacgg aattcgatct
     6061 ggccgggaac cttataagca tccaggcgtt cgcggcagag cgccagaagt tcctctgccg
     6121 tggctttttc gccgcgcttc aacacgacga ccgctttgat gaagggcgag gtatcttctc
     6181 cccccgcagt gttgttttcg ctggaggcga gggcatgacg catgttgacg acggccacct
     6241 ccagtacttt gggatgttcg tacagaacct cctccacgtc gcgcgggtag acgttatagg
     6301 aacccgccag gatcaggtct tttttgcgat caacgatggt gaagaagccg tcctcatcca
     6361 tgctggccag gtcccccgtg tgtaaccagc cgccttgcag cgtagcgcgc gtctcgtccg
     6421 gcatattcca gtagccctgc ataacctgtg ggccgcgcac gagcagctcg cccacctggc
     6481 cgggcggcag cgcttcgccg gtcttgacat ccacgatgcg cgccgaggtg ccgggcaggg
     6541 gcaggccgat actgccgacg cgccgctcgc ctttgagcag gttgcagtgc gtcgaaggcg
     6601 acgcctccgt caatccatag ccctcgacca gccgcccgcg cgtcagcttc tcgaaggctt
     6661 cctgcacctc gaccggcagc ggtgcggagc cgctcacgca ggtgcgaatg gccgccacgc
     6721 catacgagcg caccttcgga tagttggcaa ttgagagata gagcgccgga acgcccggta
     6781 aaatcgtggg atgcgactgc ttgatcgact ccatcacctg gtcgaggcgc tgggtcggca
     6841 acagcacctg tgtagcggca agcgcgatgg agagattcat acaactggtg aagccgtagg
     6901 aatgcgtcag gggcagtacg cacaacgtca cctcgcgccc gcgcttcgag tcgctgagcc
     6961 agtgacgctg ctgcgccacg ttaatcacca ggttgccgtg cgagagcatc acgcctttgg
     7021 gtgtatcggt cgtgccagag gtgtacaaca acaccgccag gtcgtcagat gtcgcgccgc
     7081 cgtcgatgag cctggccggt tggccgtgta gcaggcgctg gaacgcatac tgcgtccagg
     7141 tatgtccccc gggagccacg cgctctaccg gcacactatc ctgctctaca tgtgccgctg
     7201 ccgtaccatc ctccccaggc gttgcaggtg acgcgggcgg agcaagagcc gctttggacg
     7261 cgcccgcgcc atcgatcata ttcgccaggg ccacgcgctg gcgcaggggc aggtattcgc
     7321 gcacatcggt gtagattacc tggcggatgt cggcctgctg gcaggcgcgc tcgaccatcg
     7381 gcgcatacga ggagagcgcg agcagcattt ttgcgccaga atgctgaagc tggaaaagaa
     7441 tctcctcttc attggagagc gggctgccca gcaccaccac acctcccgcc ttgagtgtgc
     7501 catacacgcc gaccacgaac tgcggcacat tcggcaaaag aatggcgatg cggtcgccct
     7561 tgttcactcc caattcccgt aaagcgtggg cgaaacgatt gctcgagcgg tccagttcgc
     7621 gaaagctaat atgctgccca aagaagatca acgcgctgcg gctggcatac gctatcgcgg
     7681 cattgttgag caaatcggtc accagcattt tggagcgtgg gatcgtctca ggcacatcgt
     7741 tatcatagtg ttgcagccag ggcatctcca catgacgcag cgcgtgagca gacgcggcgg
     7801 agacggtaga ggagccgggc gctgcgatgg ttttttgtcg cgtatcaatg aaacggcgga
     7861 tggcgcggtt gacggcgtcg ggccgctcga gctggacaag atgcgcggag acgggtatga
     7921 cgacctgctg cgcccccgga atgctgcggg cgacatcctc gtaatgctcg cgcaggaaga
     7981 cggtatcgcg atggcccatc atgaccagcg ccgggacacg caatcgctcc aggctggccg
     8041 gtcccttcca ggtggataag gcgttgttca acatacgctt gagggtgcgc tgaggagcat
     8101 agagcgcgac cttgatgagc ttcgccacgc cgctgaagat aggatcggga acaagcatca
     8161 atcgtcgcac gatgtcacgc acaatgaacc gggaaggcac accgatcagc accaggcccc
     8221 tgacatgctc tggatggcgc agcgcgtact cggtggcgat cgccccgccg aaggagtgcg
     8281 ccagcaggta gaatggttga ggcacgttca gccgctcaat cactgcttcg agatcgtcta
     8341 ccagcgactc catcgtgcag ggaaggtcgg cgggatcatc gctgaagcca tgcccacgca
     8401 gatcatacgc gaccacgcgc atcgtttgtc cgaaactctg gagctgatac tgccactgcg
     8461 tcgcgctgcc gccatagcca tgcaccatca cgagcgtcag ctccgcgtct tccggctgca
     8521 gatcgatcac gctgatgcgc cgcagttgct ggccctgccg cccaaagggc atcatcagtg
     8581 tgcgccggta cagttcgatg tctagtgaca tgccatcctc cctcgaacgg tccagaaggg
     8641 cgaccgtaag ggtacgcccc tacaatgacc acgatggatg ctgacgggtt cagagtcatt
     8701 gtaggggcgt acccttgcgg tcgccc
//
LOCUS       DASZAK010000593         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_298262, whole genome shotgun sequence.
ACCESSION   DASZAK010000593 DASZAK010000000
VERSION     DASZAK010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298262"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="VGT44_10440"
     CDS             complement(<1..1706)
                     /locus_tag="VGT44_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581256.1"
                     /translation="MRKHFQRHIIVLLALVIALVGGIGANFVFAKGQGQSAASTVKPP
                     LVSSGGLSAASVVDVHSVDMRNVSSMTVNSSGNHAQAMDGSTGVDPAVYLQRKAAAAH
                     NARLPKLLHPYLNSTNTPFTPTPTTKFQGISDSPQTCSLGACQPSDMALATSSSWVVQ
                     GVNDAFAVYSTAGTLQAGWPILGRTFFNIPSPGSCDPTGPNLFDPRAFYDINDGRFWV
                     MMIQAEGTWAGNSCPFASIYWIAVSQTKDPRGSWFVYGVNMTLGTQNNADYPGFGFDK
                     KAVYFTANMFSNAHSFQYAEIVFGSKSLMEKGSTVSFRAFSDMMANGAAVDTVQPVET
                     ELTKSAQTEPELLINSFNINFGGGQCSSLCSGIVVWTLANPTGTKPTLTGVVISTMTY
                     GLPPFADQPACTQCLETFDVRITGTPVYANGLISFALETGLNNGTQLVPGIFWGQVQP
                     TFTSGKISGGSIAQSGYLGFAGDQAASFAAMMPRKSGNLLVVFDVMSHSLYPSIDYAT
                     RTTTDPAGTFESPLFLVTGQNSTPDTRWGDYSATSYDGFSTDNAWFAAQYTVANGDWS
                     TY"
     gene            complement(2098..3585)
                     /gene="gcvPB"
                     /locus_tag="VGT44_10445"
     CDS             complement(2098..3585)
                     /gene="gcvPB"
                     /locus_tag="VGT44_10445"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEV2581257.1"
                     /translation="MSSVEPLIFEIGAAGRRAATMTAMDVPTGSLESMIPAEMIRQQP
                     APLPEVSEIEIVRHYTHLSQRNFGVDTGFYPLGSCTMKYNPKINEDMAGLPGFARIHP
                     LQPDSMAQGAIQLIYELEQYLAEIAGMARVTLQPSAGAHGELTGLMLIKAYHERNGQG
                     HRNLVLIPDNAHGTNPASATLADYKSVEVKTDPATGGILVEHLKALLDANAANVAAIM
                     MTNPNTLGLFDANVAEIATMVHEAGGQLYYDGANANAVLGITRPGDMGFDVVHFNLHK
                     TCSTPHGGGGPGAGPIGVKEHLVPFLPGPLPAKDDTGYYWQDAGPLSIGKVRANKGNF
                     GVLVRAYTYIRTYGPDGLLHVSQSAILNANYLKHALESDYEIAYPQTCQHEFVATAER
                     QKEESDVTALDIAKRILDFGMYAPTIYFPLIVHEAMMIEPTETETRETLDYFVSVMRQ
                     IAEESRTNPDIVKSAPHTTPVGRLDQALAARKPNLRWMPGQEATH"
     gene            complement(3582..>4681)
                     /gene="gcvPA"
                     /locus_tag="VGT44_10450"
     CDS             complement(3582..>4681)
                     /gene="gcvPA"
                     /locus_tag="VGT44_10450"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEV2581258.1"
                     /translation="PHLQRRSEFVTSYTPYQPEVSQGMLQTIYEFQTMVCQITGLDIA
                     NASLYDGATALVEAVLLAVGPGGRGEVVISETVDPQYRMVLRTYAHARGFSVVEVPSR
                     DGVTSIEDLDAAVSATTAAVVIQQPNFFGCIEDVRAIEPIAHKGKAVFVTTITEPASL
                     GILAAPADYGADIAVGELMSFGNTMSYGGPALGFMAARQKYMRMLPGRLVGQTVEEGG
                     EHRTGYVLTLQTREQHIRRERATSNICTNQSLLAVGATIYMAALGKQGFRELGELCLQ
                     KAHYAARQIAALPGYTAKFSSPFFDEFVITAPVSTQALEKHLGQAGIIGGYALNRGYP
                     GMDNDMLFCVTETRTKKDIDTLVSVLKEVQP"
BASE COUNT          980 a         1317 c         1372 g         1012 t
ORIGIN      
        1 atatacgtag accagtcccc attggcaacc gtgtattgcg cagcaaacca ggcgttgtca
       61 gtggaaaagc catcatagga tgtagcgctg taatctcccc aacgagtatc cggcgttgag
      121 ttttgacctg ttaccaggaa taagggtgac tcgaaggttc ctgccgggtc ggtggtggta
      181 cgggtagcat aatcaatact cggataaagc gagtggctca tcacatcaaa cacaacaagc
      241 aagttcccgc tcttcctggg catcatcgcc gcaaaagagg cggcttgatc gccagcaaac
      301 cccaggtaac cgctctgtgc gatagaccca ccggagattt ttccactcgt gaacgttggc
      361 tgcacctgtc cccaaaaaat ccctggcaca agttgggtgc cattgttcaa ccccgtttca
      421 agcgcgaatg aaatcagtcc attcgcgtaa acaggtgttc ccgtaatcct gacatcaaac
      481 gtttccaggc attgtgtaca cgctggttgg tcggcaaatg gaggaagtcc atatgtcatc
      541 gttgagataa ccactcctgt aagagtcggt ttggtgccgg tcggattcgc cagcgtccac
      601 acgacgatgc cgctgcacag cgatgaacat tgaccgccgc cgaagttaat gttgaacgag
      661 tttatcagta gctcgggttc ggtctgagca gatttggtta gctcggtttc aactggttgt
      721 acagtatcta ccgcagcgcc attggccatc atatccgaga aggcgcggaa ggagactgta
      781 cttcctttct ccatcagtga tttgcttcca aaaacgatct ctgcatactg gaagctatga
      841 gcattggaaa acatgttggc cgtgaaatag accgctttct tgtcaaagcc aaatccggga
      901 tagtccgcat tattttgcgt ccccagcgtc atattaaccc cgtagacgaa ccagctgcct
      961 cgcggatcct tcgtctgact taccgcgatc cagtagatcg atgcgaacgg acagctattc
     1021 ccggcccatg ttccttccgc ctggatcatc attacccaaa agcggccatc attgatatca
     1081 taaaacgcgc gcgggtcgaa caaatttggc ccggtggggt cacaagaacc cggactgggt
     1141 atattaaaga aggttcgtcc caggataggc catccggctt gaagggttcc agcagtgctg
     1201 tacacggcga aggcatcatt tactccctgc accacccaac tggaagaggt cgccagcgcc
     1261 atatcggaag gctggcaggc gcccagcgag caggtctgtg gtgaatcaga tatcccctgg
     1321 aatttagtcg tcggagtggg ggtgaatgga gtatttgtcg aattcagata gggatgtaat
     1381 aatttgggaa gtcttgcatt gtgcgccgcg gctgctttac gctgcaagta aactgcgggg
     1441 tctacccctg tagacccatc catcgcttgc gcatgattgc ctgacgaatt gacggtcata
     1501 cttgatacat tgcgcatatc gaccgaatgt acatcaacaa cactagcagc ggacagacca
     1561 ccagagctaa caagcggtgg tttgacagtg ctggccgcgc tctgaccctg ccctttagca
     1621 aatacaaagt tcgcgccaat tcctcccacc agcgcgatca caagcgccag taatacgatt
     1681 atatggcgct gaaagtgctt tcgcatgtac gatcattctc ctcattacta ttcctcatgc
     1741 aaaagtaatt caagctcttg cataaggaat agtaatgagg atatatctct tttaatagag
     1801 caaaacggtt aagatatggt tgagatttaa tgagaaacgt acaagattct ttgattactt
     1861 acgctactcg tcttgtgtaa agggagccat cgcggttgtg aggggaaatg tgtgtcccga
     1921 ttgtgacatt cgcaggccaa aatgagagca ggatgtgagg aagatgcttt atagtaggag
     1981 cataggtgac aagcgaatca cccatgcgga agaaaacgat gtacttctgc aattgagcga
     2041 gggtcagaga tagggccagt gacgttcctg gtcgctgacc ctcgctcact agtatgccta
     2101 atgcgtcgcc tcctgccccg gcatccagcg caggttcggt ttgcgcgcgg ccagcgcctg
     2161 gtcgagcctg ccgacaggcg tggtgtgcgg cgcgctcttg acgatatcgg ggttcgtgcg
     2221 cgactcctcg gcgatctggc gcatcacgct gacgaagtag tccagcgtct cgcgcgtctc
     2281 cgtctcggtc ggctcgatca tcatcgcctc gtggacgatc agggggaagt agatcgtcgg
     2341 cgcgtacatc ccgaagtcca ggatgcgctt ggcgatgtcg agcgccgtca catccgattc
     2401 ctccttttgc cgttccgctg ttgccacgaa ctcgtgctgg caggtctgcg ggtaggcgat
     2461 ctcgtaatcg ctctccagcg cgtgcttgag gtagttggcg ttgaggatcg cgctctgcga
     2521 gacgtgcagc agaccatcag ggccgtaggt gcgaatgtag gtataggcgc gcaccagcac
     2581 gccgaaattg cccttattgg cgcgcacctt gccaatggag agcggcccgg cgtcctgcca
     2641 gtaatagccg gtatcgtcct tcgcgggcag cggccccggc aggaacggca cgagatgctc
     2701 cttgacgccg atgggacccg cgccggggcc gccgccgccg tgcggagtgc tgcatgtctt
     2761 gtgcaggttg aaatgcacca cgtcgaagcc catatcgccg gggcgcgtga tgcccagcac
     2821 cgcgttggcg ttcgccccgt cgtagtagag ctggccgccc gcctcgtgta ccatcgtggc
     2881 aatctcagcg acattggcat cgaaaaggcc cagcgtgttc gggttggtca tcataatggc
     2941 ggcgacattg gcggcgttgg cgtcgagcag cgccttgaga tgctcgacca ggatgccgcc
     3001 ggtggcgggg tcggtcttga cctcgaccga tttgtaatcg gccagggtcg cgcttgccgg
     3061 gttggtgcca tgcgcgttgt ctggaatcag caccaggttg cgatgcccct gcccgttgcg
     3121 ttcgtgataa gctttgatca gcatcagacc cgtcaattcg ccgtgcgcgc ccgccgaggg
     3181 ctgcaaggtg acacgcgcca tccccgcgat ttccgccagg tattgctcga gttcgtagat
     3241 caactggatc gcgccctgcg ccatgctgtc gggctgcaag gggtgaatgc gcgcgaagcc
     3301 gggcagcccc gccatatcct cgttgatctt gggattatac ttcatggtgc aggagccgag
     3361 cgggtagaag cccgtgtcca ccccaaagtt gcgctgcgag aggtgcgtat agtggcgcac
     3421 aatctcgatc tcgctgacct ccggcagggg cgcaggctgc tggcgaatca tctcggcggg
     3481 aatcatgctt tccagcgacc cggtcggcac atccatggcc gtcatggtcg cagcgcggcg
     3541 gcccgccgcg ccaatttcaa aaatgagcgg ttcgacgcta ctcatggctg cacctccttc
     3601 aaaacggaaa ccagcgtatc gatatctttc ttcgtgcgtg tctccgtaac gcaaaagagc
     3661 atgtcgttgt ccatgccagg atagccacgg ttgagcgcgt agccgccgat gatgcccgcc
     3721 tgccctaaat gcttctcaag cgcttgcgtg gagaccggag cggtgataac aaactcgtcg
     3781 aagaacgggc tgctgaattt cgcggtatag ccggggaggg cggcaatctg acgggcggcg
     3841 tagtgcgctt tttgcaggca gagttcgccg agttcgcgaa agccctgctt gcccagcgcc
     3901 gccatataga tcgttgcacc gaccgccagc agcgactggt tggtgcagat gttggaggta
     3961 gcgcgctcgc ggcggatgtg ctgctcgcgc gtttggaggg tcagcacata gccagtgcga
     4021 tgctcgccgc cctcttcgac cgtctgccca acgaggcggc caggcagcat gcgcatgtat
     4081 ttctggcgcg ccgccatgaa gcccagcgcc ggtccgccgt agctcatcgt attgccgaag
     4141 ctcatcagct cgccgacggc aatatccgcg ccatagtctg ccggcgcggc caggatgccc
     4201 agcgaggcgg gttcggtgat agtggtgacg aagacggcct tgcctttgtg cgcgatcggt
     4261 tcgatggcgc gcacgtcctc gatgcagccg aagaaattgg gctgctggat cacgacggca
     4321 gcggtagtcg cgctcacggc ggcatccagg tcttcgattg aagtgacgcc gtcgcgcgat
     4381 ggcacctcga cgacgctgaa gccgcgcgcg tgggcatagg tgcgcagcac catgcgatac
     4441 tgcggatcaa ccgtctccga gatgacgacc tcgccgcgac ctcccggtcc gaccgccagt
     4501 agcaccgcct cgaccagcgc ggtcgcgcca tcgtagagcg aggcattggc tatatccaat
     4561 ccggtgatct ggcagaccat cgtctgaaat tcgtagatgg tctgcaacat accctgactc
     4621 acttctggct ggtagggcgt gtacgaggtg acaaactcgg aacgacgctg caaatgtggc
     4681 a
//
LOCUS       DASZAK010000594         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_370824, whole genome shotgun sequence.
ACCESSION   DASZAK010000594 DASZAK010000000
VERSION     DASZAK010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370824"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..3148)
                     /locus_tag="VGT44_10455"
     CDS             complement(92..3148)
                     /locus_tag="VGT44_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2581259.1"
                     /translation="MQVEQSFASEVAIKFTGGPLTGRTVTFQKPVISFGRDGQCDVVV
                     MDPKVSRIHARIYWMNGAWMIENLSQSSYISIDRQRIQQGMIQHNSLVGLGADTSFVF
                     LVQQQAQVAPDGRLSPQQPFPPQPQSTVASEPTPVRALNSRSNPSPSETIVAPMMELS
                     VPSLVVSSNIHSGAQMHVLDKSLFNIGRDAENEISIPESIVSAQHAQIVREGSDFYIV
                     HPHPARQRTLNGLLYQGQHIRGDQQFRKQLVSGDIFRIGDEHGTLITLAYNDGKGVPA
                     EVLPKMQPIQLTAERFTIGRTGDNAIILNHPQVSAHHAVLEKIEGGYHIVDLSSTNHV
                     FVNGQRVAQQALRSGDEMRIGPYRLTYTGTELRQYDESGLIRIDALRLKKEGNNHVIL
                     LNGISLVIPPRKFVALVGGSGTGKTTLMDALNGLRPAQEGSVFYNGVDYYHNLAAFNT
                     LLGYVPQDDIVHRDLTVERALYYAAKLRLPSDFTKEQIKWRIDEVLEDVEMTERRSLL
                     VSKLSGGQRKRVSIALELLANPGIFFLDEPTSGLDPGLDRKMMALLRRLADRGRTIIL
                     VTHATNNINVCDYVCFLAQGGRLAYFGPPNEAKTYFDKTDFAEIYSTLEVTKEQPDIP
                     ARAEARFLASQDYQRYVAEPLQEGQARIAQQQSQSRPAPAGASERVKRGNAWSQFLLL
                     STRYLELLKNDKGNLFILLLQAPVIAVMLVLMVRFEIGPGIFDPGNLVQCRTQILTSS
                     GPLALPQAQQSETIDCQQVLIFLQNDPNGQQYAQSNGGVTRVLQDFILPGPGSNAQKV
                     LFILAFATVLFGCLNGAREIVKESAIYQRERAVNLGIMPYMFSKIVVLGVLCFFQSAV
                     LTLVIQLGEPLQQGIIFSPILETYITLTLTSLAGLLIGLAISAIAPNNDRAISLVPII
                     LIPQVIFSGAIIPLKDWLTQILATIFPTRWAMAALGSSIGLHADKIGGDRLFGSDYTY
                     HGTLFSIYTQADATQRLLLSWASLGVIIVVLVVIVGLFLKRKDVRV"
BASE COUNT          752 a          931 c         1033 g          735 t
ORIGIN      
        1 acagggcgac cgcatgggcc gccactacga taccggggat acgtcaaccg tccttgcgtt
       61 aacgtagtgg cgacccttgc ggtcgccatg cctacaccct gacatccttc cgtttcagga
      121 agaggccgac gatcaccacc agcacgacga ttatcacgcc cagggacgcc caggagagca
      181 gcaaacgctg ggtagcgtcg gcctgggtgt agatcgaaaa aagggtgccg tgataggtat
      241 aatcgctgcc gaagagccgg tcgccgccga tcttgtcggc atgcaggcca atcgaagatc
      301 ctagcgccgc catagcccag cgcgtcggaa aaatcgtcgc gagaatctgc gtcagccagt
      361 ctttgagcgg gatgatggcc ccagagaaga tcacctgggg gatcaggata atcggcacga
      421 gactgatggc gcgatcattg ttgggcgcga tggcggaaat ggccaggcca atcagtaagc
      481 cagccagcga ggtcagcgtg agcgtgatat aggtttccag tatcggtgag aagatgatgc
      541 cctgttgcag tggctcgccg agttgtatca caagggtcag caccgcgctc tggaagaagc
      601 ataacacacc cagtacgacg atcttcgaga acatataggg catgattccc aggttgaccg
      661 cgcgctcgcg ctggtagata gccgattctt tgacgatttc acgcgcgcca ttgaggcagc
      721 caaagagcac cgttgcgaag gccaggatga acagcacctt ctgggcatta gaacctggcc
      781 caggcaggat gaagtcttgc aacacccgcg tcactccacc attgctctgg gcgtattgct
      841 gcccgttggg gtcgttttgc aagaagatga gcacttgctg gcaatcgatc gtttcgctct
      901 gctgcgcctg tggcaaagcc agggggccgc ttgaggtgag aatctgcgtg cggcactgca
      961 ccaggttacc ggggtcaaag atacccggcc cgatctcgaa acgcaccatc aacacgagca
     1021 tcacagcgat tacgggcgct tgcaggagca ggatgaacag attgcccttg tcgtttttga
     1081 ggagttccag gtagcgcgtc gagagcagca ggaattggga ccaggcattg ccgcgcttga
     1141 cgcgctcgga agctcctgcc ggggccggtc gggactggct ttgctgttgg gctatacgcg
     1201 cctgcccttc ctgcaagggt tctgccacgt agcgctggta atcttgcgag gccagaaaac
     1261 gcgcctcggc ccgcgccgga atatccggtt gttctttcgt cacttcgagg gtgctgtaga
     1321 tttcggcgaa gtcggtctta tcgaaatagg ttttggcctc gttgggtggg ccaaagtagg
     1381 ctaggcggcc accctgcgcc aggaaacaca catagtcgca gacgttgata ttattcgtgg
     1441 cgtgcgtcac gagaatgatg gtgcgccccc ggtcggcgag ccgccgcagc agggccatca
     1501 tcttgcggtc cagaccggga tcgaggccgg aggttggctc atccaggaag aagatgcccg
     1561 gatttgccag cagttcgagc gctatcgaaa cccgtttgcg ctggccaccg gagagtttgc
     1621 tgaccagcag gctgcgccgc tcggtcatct ccacgtcttc gaggacttca tcgatgcgcc
     1681 acttgatctg ctctttcgta aagtcgctgg gcaggcgtag tttagcggca tagtacaggg
     1741 cgcgctccac ggtcagatcg cggtggacga tgtcgtcctg gggaacatag ccgagcagcg
     1801 tgttaaaggc ggcgagattg tggtagtagt caacgccgtt gtagaagaca ctgccctctt
     1861 gcgccgggcg caggccgttg agggcatcca tcaaggtcgt tttccctgta cccgaaccgc
     1921 ccacaagcgc gacgaatttg cggggcggga tgaccagcga gatgccgttg agcaggatga
     1981 cgtggttatt gccctctttt ttgaggcgca gcgcgtcgat gcgtataaga ccgctttcgt
     2041 cgtactggcg cagttcggtg ccggtgtagg tcagtcgata tgggccgata cgcatttcgt
     2101 cgccagagcg cagcgcctgt tgggcgacgc gctggccgtt gacgaagacg tggttcgtgc
     2161 tggacaaatc gacaatgtga tagccgccct caattttttc aagcacagcg tgatgcgccg
     2221 acacctgcgg atggttgagg atgatcgcat tatcgccggt acgtccaatg gtgaaacgct
     2281 cagccgtcag ctgaatgggc tgcatcttcg ggagcacttc cgctgggacg cccttgccgt
     2341 cgttataggc gagcgtgatc agcgtgccat gttcgtcgcc gatacggaag atatcaccgc
     2401 tgaccagttg cttgcggaac tgctgatcgc cgcgaatatg ctgcccctgg tacaacaggc
     2461 cgttcaaggt tcgctggcgc gccggatggg gatgcacgat atagaaatcg ctgccctcgc
     2521 gcacaatttg cgcatgttgg gcggagacaa tagactccgg gatggagatt tcgttctcgg
     2581 catcgcgccc aatgttgaaa agtgatttat ccagcacatg catctgcgcc cctgaatgaa
     2641 tgttgctgct cactaccagt gaaggcacac tcagctccat cataggcgca acgatcgttt
     2701 cggagggcga tggattggac cggctgttga gagcgcgtac tggtgtcggt tccgacgcaa
     2761 ccgttgattg aggctgtggt gggaaaggct gctgaggtga cagtctaccg tctggggcta
     2821 cttgcgcttg ctgctggaca aggaaaacaa agctcgtatc agcgcccagg ccaacaaggc
     2881 tattatgctg aatcatgccc tgctgaatgc gctgccgatc aatcgagata taactgctct
     2941 gcgacaggtt ctctatcatc cacgcgccat tcatccaata aatacgcgcg tgaattcgcg
     3001 acaccttcgg gtccatcacc accacgtcgc actgtccatc ccggccaaag gaaataaccg
     3061 gcttttgaaa agtaactgtt ctgcccgtga gtgggccacc agtaaacttg atcgctactt
     3121 cactggcaaa gctctgttcg acttgcatca aataactcca caaagacgga ttaagaagga
     3181 ttgcaatttc gttgctgacc tacgagatta tagcatgttt cctggcaatt acgagagagc
     3241 gaaaaaatga actttcgcga ccaggatcgg atatctcgta ggagccgatt atgaacatta
     3301 acaaattaag atggagataa gccaacatag tggagcagat ccgatgaggg ctgatcgtac
     3361 tgatcgagcc agtcctggac gcgctcttgc aagtccttcc agcgactgct ataacgatgc
     3421 aagtgcagca gttcctgttt gagcttgcgc c
//
LOCUS       DASZAK010000595         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_370842, whole genome shotgun sequence.
ACCESSION   DASZAK010000595 DASZAK010000000
VERSION     DASZAK010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370842"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VGT44_10460"
     CDS             complement(<1..519)
                     /locus_tag="VGT44_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581260.1"
                     /translation="MSLIRHQPLFENPFQLEDLSVFDDATLRNMLGTGGFGMTAECLG
                     RGLHGAPPLLVERFVHTMPSHLRSSFIEELRRPVPPAEVEEARQRLLDNLFWELTYWR
                     TPEWYDELTAGERLHPGIFRQLEPALCGKVVLDAGAGSGRASFECLRHGARLVYAVEP
                     SPGLSRILEQKLS"
     gene            662..1021
                     /locus_tag="VGT44_10465"
     CDS             662..1021
                     /locus_tag="VGT44_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HEV2581261.1"
                     /translation="MPDRLVYSTDGGRIATCPTCGKPYKQCRCDDPAQMPAKKSDGIV
                     RIMRDRKHRGGKTVTVVTGIPANDDAILALAQQLKKLCGTGGTVKDGVIEIQGDHCDK
                     VQARLTELGYRVKRAGG"
     gene            1014..2243
                     /locus_tag="VGT44_10470"
     CDS             1014..2243
                     /locus_tag="VGT44_10470"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2581262.1"
                     /translation="MDKQDRRDFIKLLVGQTISNVGSGITGIALPLTAVLVLAASPLQ
                     MGILSALDGLAALAFGLLAGVWVDRLRRRPIMIAADLGRMALLGSIPLAALLGSLHLA
                     QIYLVAASAAILTIFFRVANESFLPTLVPPEQLVEANSKIGTSDALAEIVGPASAGPL
                     VQLIGAPFALLFDAGSFLCSALSIWLIRKPEPQPIREEQRASAWRESIAGLRIVRENE
                     LLRALAISAATFNFFGNFVGALYILYVVRELHAPPFITGLLVAAGGVSALIGTLLARR
                     VIRRLGTGFSIGAMLFLYGLTGLLTPLASGPLALAIAMLFSAQLLGDASVAIYFIAEV
                     SLRQAIIPNAYRGRVNATMQFLSQGVAPVAALMAGILGTAIGLRLTILMGVLGVMLAG
                     ALLLLSPVRGVHEHTLQ"
     gene            complement(2328..>3835)
                     /locus_tag="VGT44_10475"
     CDS             complement(2328..>3835)
                     /locus_tag="VGT44_10475"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEV2581263.1"
                     /translation="SRSRKRVLMLELCEPEEAVGPRFATHQGQLRPSLLECAQDHSLL
                     AAHRAPIVAGDHEYFAAFATLSGPAAHAAGASSATGPDLLTHLADFAALYDYLLLVTT
                     QTTPGALVKAIAEQSSRVIVLIPAGTRAVEQQPIPLKKDQVRTVFVTHIPERPTIGAQ
                     DRYTAQLGQRVTRLVAADDALLARCWQQQQTLREMASDSDIARGVDFVARFIARQTVG
                     IAFGGGGARGFAHLGVLESLIAHHVPVDYIAACSSGIITPGMYLLGKSFAESEEIFLQ
                     IQKNIVRWRIPRTSIFSNTGLKRMLRDLCGETRFEDLATPFAMVAVDLSSRSGVVLDR
                     GLIWQAGLASTALPGVFPPVLVGDHILMDAGMHDPVPIRLVRQMGADILIASELGGQE
                     PPALTSATSWLEQAMDGASEQKGQRKQQREKRLRAPTIGDLLMRTYDLAMATIGMHSI
                     READIVIRPALHRVSLRQFSEGRKFVAAGREAVEQALPQLQARLPWLEIEE"
BASE COUNT          749 a         1163 c         1182 g          741 t
ORIGIN      
        1 ggagagtttt tgctctaaga tgcgcgacaa ccccggcgaa ggctctacgg cgtagaccag
       61 ccgggcgcca tgacgcaggc actcaaacga ggcgcgtcca ctgcctgctc ctgcatcaag
      121 caccaccttg ccacataaag ccggctctaa ctgccgaaaa ataccgggat gcagtcgctc
      181 accggcagtc agttcgtcat accattcggg cgtcctccag taggtcagtt cccaaaacag
      241 gttatccagg aggcgctggc gagcttcttc tacctcggcc gggggcacag gacggcgtaa
      301 ctcttcgatg aaggacgagc gcagatgaga cggcatagta tgcacaaaac gctctacgag
      361 aagcggtggc gcgccgtgca gaccgcgccc aagacattcc gcagtcatgc cgaaaccacc
      421 ggtgccaagc atgttccgca gggtggcatc gtcgaaaaca gagaggtctt ctagctgaaa
      481 tggattctcg aagagcggtt gatgccgaat aagactcatc ttcgcaccct cctcaattga
      541 ttatacccca ttcttctctc acgtgaagaa tatggaaatg tttcaaccgt tgagatcgcc
      601 ctccgggaac tcctgccatt tgtgctatac tattcaaaaa ttctttctga ggaactactc
      661 tatgccagat cgtctcgtct actccaccga tggcggcagg atcgctacct gccccacgtg
      721 tggcaaacct tacaagcagt gtcgttgtga cgatccggcg caaatgcccg cgaagaagtc
      781 cgatggcatt gtgcgcatca tgcgcgaccg caagcatcgc ggcggcaaga ccgtcacggt
      841 ggtaacagga atccccgcca acgatgatgc tatcctggcc ctggcacagc agctcaaaaa
      901 gctgtgcggc accggcggca cggttaagga tggcgtcatc gaaatacagg gggatcactg
      961 cgacaaggtg caggcaaggt tgacggaatt ggggtaccgg gtaaagcgcg caggtggata
     1021 aacaggacag acgcgacttc atcaaactcc tggttggtca aaccatatcg aatgtgggga
     1081 gcggcattac aggcatcgct ctgccactga ccgccgtctt ggtgcttgcg gccagtccgc
     1141 tgcaaatggg catcttgagc gccctggatg gcctggccgc gctggccttc gggctgctcg
     1201 cgggcgtctg ggtagatcgc ctgcgccgcc gtccgatcat gattgccgcc gatctcggtc
     1261 gcatggcact gctaggctcg atcccgcttg ccgcgctgct gggcagtctg cacctcgcac
     1321 agatctacct tgttgccgcg agcgcggcga tcctcaccat ctttttccgc gtggccaacg
     1381 aatcatttct gccgacgctt gtgccgccag agcagctggt cgaggccaat agcaaaattg
     1441 gcaccagcga cgcgctggcg gagattgtcg gccctgccag cgccggaccg ctcgtgcaac
     1501 tgatcggcgc gccctttgcc ctgctcttcg atgcaggctc attcctctgc tccgcgctca
     1561 gcatctggct gattcgcaaa cccgaacccc agccaatccg ggaagagcag cgggcaagcg
     1621 cctggaggga gagtatcgcc gggctgcgca tagtcagaga gaacgagctg ctgcgcgcgc
     1681 tcgccatcag cgcggccacc ttcaactttt ttggaaactt cgttggcgcg ctttacatcc
     1741 tgtatgtcgt gcgcgaattg cacgcgccgc cgtttatcac cggcctgctc gtcgcggcag
     1801 gcggagtcag cgcgctgatc gggacactgc ttgcccgcag ggtgatccga cgcctgggaa
     1861 ccggcttctc aattggcgcg atgctcttcc tgtacggctt gaccggcctg ctgacacccc
     1921 tggcaagcgg cccactcgcg cttgccattg ccatgctctt cagcgcccaa ctgcttggag
     1981 atgcatcggt ggcgatctac ttcatcgccg aggtgagcct gcgccaggcc atcatcccga
     2041 atgcctaccg gggtcgcgtc aacgccacca tgcaattctt atcgcaaggg gtcgcgccgg
     2101 ttgccgcgct gatggccggc attcttggca cggccatcgg attgagattg acgatcctga
     2161 tgggcgtgct gggggttatg ctggcgggcg cactgctgct gctttcgccg gtgcgagggg
     2221 tgcacgaaca tacattgcaa taatgcacat aaatgaaaga ccctattcac tgtcgtagga
     2281 acgcgagatg cttcgctgcg ctcagcatga catgaccgac ttggaggtca ttcttcaatt
     2341 tccagccagg gcaggcgggc ttgcagttgt ggcagcgcct gctcgacggc ttcgcggcca
     2401 gcggcgacga atttgcggcc ttcggagaac tggcgcagtg agacgcggtg caatgcgggg
     2461 cggatgacga tatccgcctc gcggatgctg tgcataccga tggttgccat ggccaggtca
     2521 taggtgcgca tcaacaaatc gccgatggtc ggcgcgcgca ggcgcttctc acgctgctgt
     2581 ttgcgctgtc ccttttgctc tgacgcgccg tccatggctt gctccagcca ggacgtagcg
     2641 ctggttagcg cgggcggctc ctggccgccg agttctgagg cgatcaggat atcggcaccc
     2701 atctggcgca ccaggcggat cggcaccggg tcatgcatac cggcgtccat caagatatgg
     2761 tcgccgacca gcacgggtgg aaagacgccc ggcagagcgg tgctggccag gcccgcctgc
     2821 cagatcaggc cgcgatccag cacgacaccg gagcgcgaac tgaggtcaac ggcgaccatg
     2881 gcgaagggag ttgccaggtc ttcgaagcgc gtctcgccgc acaggtcgcg caacatgcgt
     2941 ttcaggccgg tattggagaa aatcgaggtg cgcgggatgc gccagcgcac gatgttcttc
     3001 tggatttgca ggaaaatctc ctcgctttcg gcgaacgatt tgccgagcag gtacatgccg
     3061 ggcgtaatga tgccactgct acatgccgcg atataatcga ccggcacgtg atgcgcgata
     3121 agcgattcca gcacacccag gtgcgcgaag ccgcgcgcgc ctccgccgcc aaaggctatc
     3181 ccgaccgtct gacgcgcgat gaagcgcgcc acaaaatcga cgccccgggc tatgtctgag
     3241 tcggacgcca tttcgcgcag cgtctgttgt tgttgccagc aacgcgccag cagagcatcg
     3301 tcggcagcta ccaggcgcgt cacacgctgg ccaagctgcg ccgtgtagcg atcttgcgcg
     3361 ccgatggtgg gacgttcggg aatgtgcgtg acgaacacgg tgcgcacctg gtccttcttc
     3421 aatggaatgg gctgctgctc tactgcgcgc gtaccggcgg ggatcagcac aatgacacgc
     3481 gacgactgtt ccgcgatggc tttgacgagc gcgcctggcg tagtctgcgt ggtgacaagc
     3541 agtaggtaat catagagcgc ggcgaaatcg gcaaggtgtg tcaacaagtc aggcccggta
     3601 gccgatgatg caccggcggc gtgggcagct ggtccgctta gcgtcgcgaa cgcggcgaaa
     3661 tattcatgat ctccagcgac gatgggcgcg cgatgagcgg caagcaggct atgatcctgg
     3721 gcgcattcca gcagcgatgg gcgaagctgc ccctggtgtg tggcgaatcg agggccaacg
     3781 gcctcctccg gctcgcatag ttccagcatg aggacgcgct tgcggctgcg cgagg
//
LOCUS       DASZAK010000596         6010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_15298, whole genome shotgun sequence.
ACCESSION   DASZAK010000596 DASZAK010000000
VERSION     DASZAK010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15298"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGT44_10480"
     CDS             <1..344
                     /locus_tag="VGT44_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2581264.1"
                     /translation="ERTAFGQKIGSFQNSRFKLAEMKTEIEIGRVFVDRCIEELNAGT
                     LTPEVAAMAKWWTSDLQKRVVDQCVQLFGGYGYMLEYPIARAYLDARVQSIYAGTNEI
                     MKEIIGRSLGV"
     gene            complement(395..2599)
                     /locus_tag="VGT44_10485"
     CDS             complement(395..2599)
                     /locus_tag="VGT44_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEV2581265.1"
                     /translation="MTQVEESLRFPQNVIMQQSDPHLRDETTNERAEAPTALRKLASS
                     YEVLRAVITHAPIILFVLDSNGIVTFSEGQALQGLGRKPGSAVGASIFELYHDIPEVL
                     SYICRALDGETFTAIAHMKDRIFETRYIPQFDVCDEVCEVIGVATDITERIKTEEALT
                     ASERKFRALIEEGTDLVFIISPSGVFRYASPSHQRLLGYEPESLIGRNVFELIHPKDK
                     KNVHICWEAGFEGNGALARTQCRLLHVDGSWITVEAVGRNCFDDPEIQGFVVSARDIT
                     ERIMMEEQLRYLALHDTITDLPNRAYLIEQMEQTLRATPPQAVTLLILNLNRFKDIND
                     TFGHQQGDRLLRAVGERVKQACPDASVVARIGGDEFTIVLPEVGQEAVHKAVAALHSV
                     MEEPFVVEEHPVQLDISIGGVFSPEHGREPITLLRKADIAMQKSKQAHLQFALYDASY
                     EQNTARRLDLIRELRHAISGDEFRLYYQPKVEMKSGSVYGVEALIRWQHPTLGFIPPD
                     QFISLAEQTGLIVPLTRWVLQAALRQCHDWIHDGIDLQIAVNLSMWDLRDPSLVSAIG
                     DLLRHYHVPADHLRVELTESVAMTDPDHTLAVLKQLANAGVDSSIDDFGTGYSSLAYL
                     KRLVANELKIDRSFVRHIAEETADATIVGSTVHMAHCLGLKVVAEGIEDEASMQLLNE
                     LDCDIAQGYFFARPLPPLELTQWLKERQKSMATASIATTITPESFTREPALS"
     gene            complement(2689..3828)
                     /locus_tag="VGT44_10490"
     CDS             complement(2689..3828)
                     /locus_tag="VGT44_10490"
                     /inference="COORDINATES: protein motif:HMM:NF021498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetD
                     domain-containing protein"
                     /protein_id="HEV2581266.1"
                     /translation="MITPAEIRQKAARLYPVFLRAWLQTEAFFPLTFPVGKLPTDYLE
                     LRKWARDLQAQSKEQRDSGYTLEYEVQQKRFSGEQTLPARVVIETEQDYLRLVEKQAE
                     FVAYRQDVTLIRELLPQLEAWMQRYPQRVVEHHVDWVGLLAMCRYFLEHPRPRVYIRE
                     LPINVHTKFIEQHRGIARELLEQILPPEAMTDAAETFEQRFGLREKEPVVRARFLDGQ
                     LDRRYGLPITELSLPTSQFAALDLLRGQRCIVTENEMTFLTLPPRQESFALFGGGFMV
                     RNLARVPWLADCPISYWGDLDAQGFQILSSLRSIFPHVTSLMMDWETLSTFAEFTVTS
                     IPCPVQQLPHLTPDEHDLFTHLAEHCLRLEQERISHSYALERISR"
     gene            complement(3812..>6010)
                     /locus_tag="VGT44_10495"
     CDS             complement(3812..>6010)
                     /locus_tag="VGT44_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEV2581267.1"
                     /translation="DEPTFLANAQQATALLSQAAQRIAALTNQRDEQKQRETALAATL
                     RGLAEELASLQQRKSQIPGEDIRLRAMLAETLDIPENELPFIGELLRVRDSARQWEPV
                     IERLLHSFGRQLLVPEQHYHQLSRYVDETNLRGRLIYHRIRGARTPRNNGTPDRALLF
                     YKLEVKPETEFTNWLTAELIDSYDYACCDTLEAFQQARRALTSNGQIKHSVFRHEKDD
                     RATLGDRKRYVLGWSNAEKIEAIKTELTALQASLDRVKADITRIEQLQKQEQKREQAL
                     HRLLEFTSFASIDWRSDQQRRSDLQMQQRELEASADHLAELRRQLDEVTRQHRDRQQE
                     RDRLNANITTLENAATSYMQQRAACRVTLQAASHIDLSAFVAQIEKDHKNSTLAELTI
                     ENADDAREKMAQFYRARATSLRGMLTPLISRIIGLMNDFKQISPTLALDLDAAIEAVE
                     AYRHHYEQIRREDLPRYRKRFKELLDEKVITNISVFKAALERQIEDIKKSIGSLNDSL
                     RLIDYTPSTYIQLCFDPALDSEVRDFRTQLRACLPDVTQPRTAEANEASFQRIQSLIR
                     RFEQEERWSAKVTDVRNWLDFSAEERYREDDTTKNYYNDSSGKSGGQKTKLAYTILAS
                     AIAYQYGLDEQDERAQAFRFVVIDEAFVRSDEMNARYAMELFKQLDLQLLVVTPLDKI
                     HVIEPYITACHYVTNNQEENDSRVYNFTIEEYLEHKQALQAGMAMYDYAR"
BASE COUNT         1081 a         1629 c         1939 g         1361 t
ORIGIN      
        1 aagagcgcac cgcctttggc cagaagatcg gcagcttcca gaattcgcgt ttcaagctgg
       61 ccgagatgaa gacggagatc gagatcgggc gcgtctttgt tgatcgctgc atcgaagagt
      121 tgaacgccgg gacgctcacg ccagaggtgg cggccatggc gaaatggtgg acatccgatt
      181 tgcagaagcg cgtcgtcgat caatgcgtgc agttgttcgg cggctatggc tacatgctcg
      241 agtatcccat cgcgcgcgcc tacctcgacg cgcgcgtgca gagcatctac gctggcacca
      301 acgaaatcat gaaagagatc attgggcgtt cgctaggcgt gtaagggcat ggggtacgca
      361 gggcgcggga gcatggcgac ggccagcatc gccactacga taacgcgggt tctctcgtga
      421 atgattctgg tgttatcgta gtggcgatgc tggccgtcgc catacttttc tgccgctcct
      481 ttaaccattg tgtcaattcc aggggcggca gcggacgcgc gaaaaagtag ccctgcgcga
      541 tgtcgcagtc caactcgttt agaagttgca tagatgcctc gtcctcgatg ccttccgcga
      601 cgactttgag gccgaggcag tgcgccatgt ggacggtcga gccgacgatg gtggcatctg
      661 ctgtctcctc tgcgatgtgg cgcacgaagg aacgatcgat ctttaactcg ttggcgacca
      721 ggcgtttcag gtaggcgagc gaggagtagc cggtgccgaa atcatcgata gagctgtcga
      781 cgcccgcgtt cgccagttgt ttgaggaccg ccagcgtatg atccgggtcc gtcatggcga
      841 cgctttccgt caattcgacg cgcaggtggt cggcaggaac gtgatagtgc ctgagcaggt
      901 cgccgatggc gcttaccagc gacgggtcgc gcaggtccca catggagagg ttgacggcga
      961 tttgcagatc gatgccgtcg tgtatccagt cgtgacattg ccgcagggcg gcttggagga
     1021 cccagcgcgt caatgggacg atcagacccg tttgttcggc cagcgagatg aactggtcgg
     1081 gcgggataaa gccgagcgtt ggatgctgcc agcgaatgag cgcctcaacg ccgtagacgc
     1141 taccgctttt catctccact ttgggttggt agtagagcct gaattcgtcg ccactaatgg
     1201 catgacgcag ttctctgatg agatcaagac gccgggcggt gttttgttcg tagctggcat
     1261 catacagtgc gaattgcaga tgtgcctgct tcgacttctg catggcgatg tcggccttgc
     1321 gcagcagcgt gatcggttcg cggccatgct ccgggctgaa gacgccgcca atgctaatat
     1381 cgagctgaac cgggtgttcc tcaacaacga acggttcttc cataacgctg tgtaaggccg
     1441 caacggcctt gtggactgct tcctgaccga cttcgggcaa cacgattgta aattcgtctc
     1501 ctccaatgcg tgcgacgaca ctggcatcag gacaggcctg cttgactcgt tctcccactg
     1561 cccgtaggag cctatcaccc tgctggtgtc caaaggtatc gttgatatcc ttgaagcgat
     1621 tcagattgag aatcaggagg gttactgctt gcggcggagt tgctctcaac gtctgctcca
     1681 tttgttcgat caggtaggcg cgattcggca aatcggtgat agtatcgtgc agcgccaggt
     1741 agcgtaattg ctcttccatc ataatgcgct cggtgatatc gcgtgcgctg acgacgaagc
     1801 cttggatctc aggatcgtca aagcaattgc gtccgaccgc ttcaacagtg atccacgaac
     1861 catcgacatg aagcaatcgg cactgggtgc gagccagcgc gccgttcccc tcgaagccgg
     1921 cctcccagca gatatggacg ttctttttgt cttttgggtg aataagctcg aagacgttcc
     1981 ggcctatcag ggattctggc tcgtagccca gcagccgttg gtgtgaaggg ctggcatagc
     2041 gaaagacacc tgatgggctg atgataaaaa cgaggtcggt tccctcttcg atcaatgcgc
     2101 ggaatttccg ttcgctggcc gtgagcgctt cttctgtttt gatgcgttct gtgatgtcgg
     2161 ttgcgacgcc gatgacttca catacctcgt cgcatacatc aaattgtggt atatagcggg
     2221 tctcgaatat tctgtctttc atgtgcgcga tcgctgtgaa ggtttcgcca tccagcgcgc
     2281 ggcagatgta ggagagtact tctggtatat cgtgatagag ttcaaagata gaggcgccga
     2341 ctgcgctgcc cggttttctg cccagccctt gtagcgcctg cccttctgaa aatgtgacaa
     2401 tcccgttgga atcgagcacg aaaaggatga taggcgcgtg ggtgataacg gcgcgcaaaa
     2461 cttcgtatga agatgccagt ttccgcaaag ccgtcggcgc ttccgctcgt tcattagtcg
     2521 tttcgtcgcg cagatgggga tcgctttgct gcataataac gttttgtgga aaacgaaggg
     2581 actcttctac ttgtgtcata agcgctttca ccagtgagat agccatagac aacacgggtg
     2641 gaacacctcc actcattagt gttgttatcg gcagggttgg cggaaaggtt aacgggagat
     2701 gcgctccagc gcgtagctat ggctgatgcg ctcctgctcg aggcgcaggc aatgttcggc
     2761 gaggtgggtg aagaggtcgt gttcgtcggg tgtcaggtgg gggagttgct gtaccgggca
     2821 ggggatgctt gtgacggtga attcggcgaa ggtggagagc gtttcccaat ccatcatgag
     2881 cgaggtgacg tgcgggaaga tggagcgcag gctcgatagg atctggaagc cctgcgcgtc
     2941 caggtcgccc cagtaggaga tggggcaatc ggccagccag gggacgcggg ccaggttgcg
     3001 caccatgaag ccgccgccga agagtgcgaa gctctcttgc cggggcggca gtgtgaggaa
     3061 ggtcatttcg ttttcggtaa cgatgcagcg ctgcccgcgt agcagatcga gcgcggcaaa
     3121 ctgcgaggtc gggaggctca attcggtgat cggcagccca tagcgccggt cgagctgacc
     3181 gtcgaggaag cgcgcccgca ccaccggctc tttttcgcgc aggccgaagc gctgctcaaa
     3241 cgtctctgcc gcatctgtca tggcctctgg cgggagaatc tgttccagca gttcgcgcgc
     3301 gatgccgcga tgctgctcga taaacttggt atggacgttg atcggcagtt cgcggatata
     3361 tacgcgtggc ctggggtgtt ccaggaagta gcggcacatg gcgagcagtc cgacccaatc
     3421 gacatgatgc tcaaccacgc gctgcgggta gcgctgcatc catgcttcca actgaggcag
     3481 tagctcgcga atcaacgtga catcctgccg gtaggcgacg aactcggctt gtttctcaac
     3541 caggcgaagg taatcctgct ccgtctcgat gacgacgcgc gccggcagcg tttgttcgcc
     3601 ggagaagcgt ttctgctgca cttcgtattc cagtgtgtag ccggagtcgc gctgctcctt
     3661 cgactgcgcc tgcaggtcgc gcgcccactt gcgcagctcc aggtagtccg ttggcagctt
     3721 gccgaccgga aatgtgagcg ggaagaatgc ctccgtttgc agccatgcgc gaaggaatac
     3781 cgggtagagc cgcgccgctt tctgccgtat ctcagcgggc gtaatcatac atcgccatcc
     3841 ctgcttgcag cgcctgtttg tgttcaaggt actcttcaat ggtgaagttg tagacccgcg
     3901 agtcgttctc ttcctggttg ttggtgacgt agtggcaggc cgtgatgtat ggctcgatca
     3961 cgtgaatttt gtccagcggt gtgacgacca gcagttgcag gtcaagctgc ttgaagagtt
     4021 ccatagcgta gcgcgcgttc atctcgtccg accgcacgaa cgcctcgtcg atcacgacga
     4081 agcggaaggc ttgcgcgcgc tcgtcctgct cgtcgagacc gtactggtag gcgatggcgg
     4141 aagccaggat ggtgtaggcc agcttggtct tctgcccgcc ggatttgccg gaggagtcgt
     4201 tgtagtagtt cttggtggtg tcgtcttcgc gatagcgttc ctcggcggaa aagtcgagcc
     4261 agttgcgcac gtccgtcacc ttcgctgacc agcgttcttc ctgctcgaag cgccggatga
     4321 gggactggat gcgctggaag ctggcctcgt tcgcctcggc ggtgcgcggc tgcgtcacgt
     4381 cgggcaggca ggcgcgcagc tgcgtcctga agtcgcgcac ttcgctatcg agcgccggat
     4441 caaagcagag ctggatgtag gtcgatggcg tgtaatcgat caggcgcagc gagtcgttga
     4501 ggctgccgat gctcttcttg atgtcctcga tttgccgctc cagcgccgcc ttgaagacgc
     4561 tgatgttggt aatcaccttc tcatcgagca attccttgaa gcgcttgcga tagcgcggca
     4621 ggtcctcgcg gcgaatctgt tcgtagtgat ggcggtacgc ctcaaccgcc tctatcgctg
     4681 catcgagatc aagggcaagc gtggggctga tctgcttgaa atcgttcatc aggccgatga
     4741 ttctgctgat gagcggcgtg agcatgcccc gcagcgaggt agcgcgggcg cggtagaact
     4801 gcgccatttt ctcgcgcgca tcatcggcgt tttcgatggt cagctcggcc agcgtggagt
     4861 ttttgtggtc tttctcgatc tgcgcgacaa aggccgagag gtcgatatgc gacgcggctt
     4921 gcagcgtcac gcgacaggcg gcgcgctgct gcatatagct ggtcgcggcg ttctcaagcg
     4981 tggtgatgtt ggcgttgagg cggtcgcgct cttgctggcg gtcgcggtgc tgccgtgtga
     5041 cctcgtcgag ctggcggcgc agttcggcca ggtgatcggc gctggcttcg agttcgcgct
     5101 gctgcatctg tagatcgctg cggcgctgct ggtcgctgcg ccagtcgatc gaggcgaacg
     5161 aggtgaactc aagcagccgg tgcagagcct gctcgcgttt ttgctcctgc ttctgcaatt
     5221 gctcgatgcg cgtaatgtcg gccttgacgc gatcaagcga cgcttgcagg gcggtgagtt
     5281 cggtcttgat cgcctcgatt ttctcggcgt tgctccagcc cagcacgtag cgcttgcggt
     5341 cgcccagggt ggcgcggtcg tctttctcgt ggcggaagac gctgtgcttg atctggccgt
     5401 tgctggtgag ggcgcggcgc gcctgctgga aagcttccag cgtgtcgcag caggcataat
     5461 catacgagtc gatcaattcg gcggtcagcc agttggtgaa ctccgtctcc ggtttgactt
     5521 ctaatttgta gaagagcagc gcgcggtccg gcgtaccgtt gttgcgtgga gtgcgcgcgc
     5581 cgcgaatgcg atgatagatc aaccgcccgc gcaggttggt ttcgtctacg taacggctga
     5641 gctggtggta gtgctgttcc ggcacgagca gctgccgccc gaagctgtgc agcaggcgtt
     5701 cgatgaccgg ctcccactga cgcgcgctgt cgcgcacgcg cagcaattcg ccgatgaagg
     5761 gcagttcgtt ttcaggaatg tcgagcgttt ctgccagcat cgcgcgcagg cggatatcct
     5821 cgccaggaat ctggctcttg cgctgttgca gcgaggccag ttcttcagcc agcccgcgca
     5881 gcgtggcggc gagcgctgtt tcgcgctgct tctgctcgtc gcgctggttg gtgagcgcgg
     5941 cgatgcgctg cgcggcctgg gatagcagcg cggtggcctg ctgtgcgttg gcgaggaagg
     6001 ttggttcgtc
//
LOCUS       DASZAK010000597         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_298314, whole genome shotgun sequence.
ACCESSION   DASZAK010000597 DASZAK010000000
VERSION     DASZAK010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298314"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGT44_10500"
     CDS             <1..285
                     /locus_tag="VGT44_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581268.1"
                     /translation="SLRTDPPPDYYLVLAGPKAGANALHSLLNPWVIESVYLFDAREL
                     LTALHERGVQIGNRTSVIGELWERAEIFPVQHNTRLVLTGDQRKLLTLFG"
     gene            480..>2500
                     /gene="lon"
                     /locus_tag="VGT44_10505"
     CDS             480..>2500
                     /gene="lon"
                     /locus_tag="VGT44_10505"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEV2581269.1"
                     /translation="MPIRNTVLLPNVVTPLIVGREQSVKAIEDAISKDRTLFVVSQVN
                     DELEDPGPADLYTVGVEGMIDRVLKLPDGSTSVLLHGQQRLRMLGYTRKVPFMRVRAE
                     IVHEETERTLAIEALVRAALVLFEKCVKLSSTLPEEAYITAMNIDQPGWLADFIVSAL
                     ESPVAVRQSILEAFDVEERLQKTCILLNKELNILELENKIHSQVQQEVDKSQREYFLR
                     EQLKAIQRELGETYPTMRENAELREKILSLGMPEEVQARAFKELERLNATPSMAPDGG
                     VIRTYLDWLVSLPWDKATVDRLDIKEAARILEEKHYGLQKVKERILEYIAVRKLSDNM
                     RSPILCFVGPPGVGKTSLGRSIAEALNRKFVRLSLGGIHDEAEIRGHRRTYVGALPGR
                     IIQTMKNAGTVNPLFVLDEIDKIGADFRGDPSAALLEVLDPEQNHAFSDHYLEVPYNL
                     SKVIFLTTANVLHPVPAALRDRLEVIELPGYTEEEKMHIARQFLIPKQMTEHGLKPSR
                     VEIDDEALQRIIREYTFEAGVRNLEREIASVMRKVARKVAEGRHTKTKVMAEKISDYL
                     GPQKHYYGLAEEQDEPGIATGVAWTSAGGDLMTVEATLLEGRGNLTLTGQLGDVMKES
                     AQAALSYARSRAGQLGVESRFYEKYDIHLHLPAGSIPKDGPSAGITMATA"
BASE COUNT          670 a          646 c          666 g          518 t
ORIGIN      
        1 agcttaagga cggacccacc accagattac tatctcgtgc ttgccgggcc aaaggcgggt
       61 gcgaatgcac tgcacagcct cctcaatccc tgggtgattg agtcggtcta cctttttgat
      121 gcaagagaac tgctaacagc attgcacgag cgcggcgttc agataggtaa cagaacaagc
      181 gtcatcgggg aactctggga gcgagccgaa atatttcctg tacaacataa tactcgatta
      241 gttcttaccg gggatcaacg aaaactgctc actctcttcg gataatagga cttttctgaa
      301 tcgtcgtata aaactaaacc tttagggctt gtaatgcgtt ataattcaag aacacagaat
      361 aaacaatttc cgttaagagg ggatgtatgg tcacaaagcc gaccactcgc acagctacga
      421 ggaaacgcac aaaagacaaa agcaacttac cagacaagaa cgtactggag ctgcccgttt
      481 tgccaatccg caacacggtc ctgttaccca atgtagtcac gcctttaatt gtggggcgcg
      541 aacaatcggt aaaggccatc gaagatgcaa tcagcaagga tcgcaccttg tttgttgtgt
      601 cacaggtgaa tgacgagttg gaggatccag gcccagccga tctttacacc gtcggagtcg
      661 aaggcatgat tgaccgggta ctgaagttgc cggatggttc aaccagcgtc cttctccacg
      721 gtcagcaacg tttgcgcatg ctcgggtaca cccgaaaagt gccctttatg cgtgtgcgcg
      781 cagaaattgt gcacgaagaa acagagcgta ccctggctat agaagcattg gtgcgggcag
      841 ccctggtgct cttcgaaaaa tgcgtgaaac tgagcagcac gctacccgaa gaagcttaca
      901 tcactgcgat gaatatcgat caacctggat ggctggcaga ttttattgtc tccgcgctcg
      961 aatcacccgt cgcggtgcgt cagagcatcc tggaagcctt cgatgtcgag gagcgattac
     1021 aaaagacatg tatcctgctc aacaaagagc tgaatattct ggagctagaa aataaaattc
     1081 atagccaggt gcagcaggaa gtcgataaga gccagcggga atattttctg cgcgaacagc
     1141 tcaaggctat acagcgagaa ttaggcgaga cctatccaac tatgcgcgaa aatgcggaat
     1201 tgcgcgagaa gatcctctct ttgggcatgc ctgaagaagt ccaggccagg gcatttaaag
     1261 agctggagcg ccttaacgca actccctcta tggctcccga tgggggagtg atacgtacct
     1321 acctggattg gctcgtcagc cttccctggg ataaagccac cgtagaccgg cttgatatca
     1381 aggaagcagc gcgcattctc gaagagaaac actacggcct gcagaaagtg aaggagcgca
     1441 ttcttgagta tatcgcggtg cgcaagctct ccgataatat gcgcagtcct attctttgtt
     1501 ttgtagggcc tccgggcgtt ggcaagacat cgcttggccg ttcaattgct gaggcactca
     1561 atcgaaagtt cgtgcgccta tctctgggag gcatacacga tgaagccgag attcgcgggc
     1621 atcgccgtac ctacgttggc gctctgccag gccgcatcat ccaaaccatg aaaaatgcag
     1681 gcaccgttaa cccgctgttc gtactggatg agatagataa gatcggggcc gatttccgag
     1741 gtgatccctc agcagccttg ttagaagtgc tcgaccctga gcaaaaccac gccttctccg
     1801 atcactatct tgaggtgccc tacaacctct caaaggtcat cttcctgaca acggccaatg
     1861 tgctgcatcc cgtccccgca gccctgcgcg accgcctgga ggtcattgaa ctgccagggt
     1921 atacggaaga agaaaaaatg cacatcgccc gccagttcct cattcccaaa cagatgacgg
     1981 aacacggcct gaagccatcg cgcgtggaaa tcgacgacga ggcattacag cgtatcattc
     2041 gcgagtatac attcgaagcg ggtgtacgta accttgaacg cgaaatagcc tcggtgatgc
     2101 gcaaagtagc ccgcaaagta gcagaaggac gacacaccaa aacgaaagtg atggcggaga
     2161 agatcagcga ttacctgggt ccacaaaaac actactatgg gctggcagag gagcaagacg
     2221 aaccgggcat agcaacagga gtagcgtgga cgagcgcagg cggcgacttg atgaccgtcg
     2281 aagctacgct gctggagggg cgcggcaatt taacactcac cgggcaactc ggcgatgtaa
     2341 tgaaagaatc ggcccaggcg gcgctttctt atgcccgctc gcgtgcaggg caactgggcg
     2401 tcgagagccg tttctatgaa aagtacgata ttcacctgca tttacctgca ggttcgatac
     2461 ccaaggatgg gccatcggca ggtatcacga tggcaactgc
//
LOCUS       DASZAK010000598         4078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_370969, whole genome shotgun sequence.
ACCESSION   DASZAK010000598 DASZAK010000000
VERSION     DASZAK010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370969"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VGT44_10510"
     CDS             complement(<1..715)
                     /locus_tag="VGT44_10510"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV2581270.1"
                     /translation="MRAAVFYGPNLPLRVEDMPVPAVEAGEVLVKVAACGLCHTDLHY
                     IDHGVPTVKPPPMILGHETSGTVVLTDPAAGAIKEGDRVLLPAILTCGQCAACRYGRE
                     NICQHMRMFGNDVDGAYAEYVKAPAKDIFVLPPEIPLVEGCIIADAVTTPYHAVKNRA
                     EVRPGDSVVVFGCGGVGLNVVQFAHLAGGIVIAVDIVEERLAWARRLGASLTVNPRQV
                     EGGAARKIRQLTGGGADVAL"
     gene            complement(748..1707)
                     /locus_tag="VGT44_10515"
     CDS             complement(748..1707)
                     /locus_tag="VGT44_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV2581271.1"
                     /translation="MPNFSGKDPGSFGFEEIVYRKRGWVATVTINRSESYNAYTWRTL
                     QEMTQAFRDASWDDHIAVVVLTGAGDKAFGTGGDVKEYASEYTQRPHDYWKWMGLFTD
                     CIDILRNIGKPTIARLNGMVVGGGNEFNMACDLAIAADHVRIRQVGTEVGSVAAGGAT
                     QWLPLMVGDRRAREILWLCEWIDAPTALAWGLVNQVVPYAELDDAVATMAAKLVDKFP
                     ECMRYTKQQTNFWKDFSWHQTIGHARDWLALHYTSLEPYEGMHAFVEKRRPDHLGVRQ
                     RAMTEESSEFLWGPYTQTCLACGAKGIPAQFAYCGRCGAELQE"
     gene            complement(1732..1995)
                     /locus_tag="VGT44_10520"
     CDS             complement(1732..1995)
                     /locus_tag="VGT44_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581272.1"
                     /translation="MSTGIPQARSIQLQEQGWMRRFTAMGRRLNEAVELYGQLGFETL
                     LEPADLDEEDLSRAEGCEHCIVTTLARTIYTRSRRRVDADEYS"
     gene            complement(2024..2509)
                     /locus_tag="VGT44_10525"
     CDS             complement(2024..2509)
                     /locus_tag="VGT44_10525"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2581273.1"
                     /translation="MGDQYPTTAHFNRYDVSIRAMEHADLAVVVELDALVFGVMRSAY
                     FERRLAALNEHDADARMAFLVADYQGAVIGFVMGTLAYGEFGLTQVTAILDSIAVHPG
                     YQQQGIGRQLIESFIKQGTLQGAAAVYTLVNWDNWTLLKVFHALGFVLASTIPLERRI
                     D"
     gene            2840..3979
                     /locus_tag="VGT44_10530"
     CDS             2840..3979
                     /locus_tag="VGT44_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV2581274.1"
                     /translation="MSTRVDVSLPLVLVAHHTRETVELLKALMEGEGYAVLCAYHGRS
                     ALQYALQHHPALMLFDQALPLIDGLELCRTLRQEENNAVIFLLNDHPDELGNLLAFAA
                     GADECLSLPFHPRELLARIKAVLRRTERQVASPHRLLRCGSLELDSEQREVRVAGQVI
                     GVTSLEYELLYVLLRTPGRTFSRAQLLELIPGFQHRSPFDRAVDIHISNLRRKLSQVM
                     GTDALIETVRGVGYRLSVPTTETVAEPASPGPVPTQLALAAFERAPVPLLVLAADRTV
                     VLYNEAARHLCGWSADQVVGQAKCYSLLSCHLANGTMLCREQCVMNAITLNRLSDQTA
                     CYSITLKDGREVPVTAHYSSLDEPGMDGGYTMLALAPDLAGTYLP"
BASE COUNT          835 a         1229 c         1120 g          894 t
ORIGIN      
        1 ccagggcgac atctgcgccg ccgccggtca attgccgtat cttgcgggca gcgccgccct
       61 ctacctggcg gggattcacc gtcaggctgg cgccaaggcg acgcgcccat gccagtctct
      121 cctctacaat gtctacagcg atgacgatgc cgccggccag gtgagcaaac tggacaacat
      181 tgaggcctac cccaccgcag ccaaagacga ctacgctatc tccaggccgc acctcggcgc
      241 gattcttgac ggcgtgatag ggcgtggtca ccgcgtcggc gatgatgcag ccctccacca
      301 gtgggatttc aggcggcagg acgaagatat ccttagcggg cgccttcacg tactcggcat
      361 aggccccatc cacgtcgttg ccgaacatgc gcatgtgctg gcagatattc tcccgcccgt
      421 accgacaggc ggcgcattgg ccgcaggtga ggatggccgg cagcagcacg cggtcccctt
      481 ctttgatagc gcccgcggca gggtcggtca atacgacggt gccgctggtc tcgtgcccaa
      541 gaatcatggg tggcggtttg acggttggca caccgtgatc gatatagtgc aggtcggtgt
      601 ggcagaggcc gcacgctgcc actttcacca gcacctcgcc cgcctctact gctggcacag
      661 gcatatcctc aacgcgcaat ggcaaattcg gtccatagaa aacggcagca cgcatctctc
      721 tctccctcca ttgctcgatt cgtctcacta ctcctgaagt tctgcgccgc agcggccgca
      781 gtaagcgaac tgtgctggaa tgcctttagc gccgcatgcc aggcaggtct gcgtatacgg
      841 accccagaga aactcgcttg actcttccgt catcgctcgc tggcgcacgc ccaggtgatc
      901 gggccgccgc ttctcgacga aggcatgcat gccctcgtac ggttccaggg aggtatagtg
      961 cagggcaagc caatcgcgag cgtggccgat ggtctggtgc caggagaagt ctttccagaa
     1021 atttgtttgc tgtttggtgt agcgcatgca ctcggggaac ttgtctacca gtttggcggc
     1081 catcgtagcg acggcgtcgt caagctcggc gtaggggaca acctggttga ccaggcccca
     1141 ggcgagggcg gttggggcat cgatccattc acacagccac agaatctcgc gcgcgcgccg
     1201 gtctccaacc atcagcggca gccactgcgt ggcgccaccc gcggcgacgc tgcccacctc
     1261 ggtgccgacc tggcgaatgc gcacgtgatc ggccgcgatg gccaggtcac aggccatgtt
     1321 gaactcattg ccaccaccga caaccatgcc gttgagccgg gcaatcgtgg gcttgccaat
     1381 gttgcgcagg atatcaatgc agtccgtgaa aagtcccatc cacttccagt aatcgtgtgg
     1441 tcgctgcgtg tactcggagg catactcctt gacatcgcct ccagtgccga atgccttgtc
     1501 acccgcgccg gtcagcacca cgacggcgat gtggtcatcc caggaagcgt cgcggaaggc
     1561 ctgcgtcatc tcttgcagcg tgcgccaggt gtaggcgtta tagctctccg agcggttgat
     1621 ggtcacagtt gccacccagc cacgtttgcg atacacgatc tcctcaaagc caaaactccc
     1681 agggtctttc ccgctaaaat tcggcatttt cttcctccct taaggcacct atcatgaata
     1741 ttcatcagca tcgactctcc tacgagaccg ggtgtaaatg gtgcgtgcca gggtagtgac
     1801 gatacagtgc tcgcatccct ccgcacgaga tagatcttcc tcatccaggt ccgccggctc
     1861 cagcaaggtc tcgaatccaa gttgaccata cagctccacg gcttcgttta accgtcgtcc
     1921 catggcggtg aagcgccgca tccagccctg ctcctgtagt tggatgctac gcgcctgtgg
     1981 tatgccggta ctcatcgcta atgcactccg tgaaggtggc tgtttagtca atccgccgct
     2041 ccagtggaat ggtcgaagct aacacaaaac caagcgcatg aaacactttg agcagggtcc
     2101 agttatccca attcaccaac gtgtagacag ccgccgctcc ctgaagtgta ccctgcttga
     2161 taaagctttc gatcaattgc cgaccaatgc cttgctgttg atagcctgga tgaactgcaa
     2221 tggaatcaag aatagcagtc acctgcgtaa gaccgaactc accatacgcc agcgtaccca
     2281 tcacaaagcc aatgaccgca ccctgatagt cggctactaa aaaagccatg cgcgcgtcag
     2341 cgtcatgctc attgagggcg gcaagccgcc gctcaaaata ggctgatcgc atcaccccaa
     2401 agaccagggc atccagttcg acgacaactg ccaggtccgc gtgctccatt gcacgaatgg
     2461 acacatcgta acggttaaag tgtgcagtcg ttgggtactg atcgcccata ctccatttcc
     2521 ttccctgata gtagaccggc accagttcca aagcctgacc atacggccgt gtcggcatcg
     2581 gctgccatgg ctatggccct tgccttacgc aggttctaat gtcgatgata cggtgaatgt
     2641 gtgtagattg ttcgtgaaga attgtatggt ttttgtaaag ctgaaattag tgtgctcgct
     2701 tgcttatcct tgacgcgcga gcgcgcatac gatatagtgt tcatcatagg aattgttttg
     2761 tagcgttgaa gcgcgcggtt gtctgcttgt ttttcgggct agtcaggttc gctgtcaggc
     2821 gaacagagag gctcccgtca tgtcaacgcg agtcgatgtt tcgctccccc ttgtcctggt
     2881 ggcccaccat acgcgggaga cagtcgagtt actgaaagcc ttgatggaag gtgaaggcta
     2941 cgcagtcctg tgcgcctatc atggtcgttc tgctttacaa tacgctctcc agcatcaccc
     3001 ggccctgatg ctcttcgacc aggccctgcc cctgatcgat ggtttggaac tctgccgcac
     3061 attacgacaa gaagaaaata acgctgtcat ctttctgctc aatgaccacc ccgatgaact
     3121 cgggaatctg ctggcttttg ctgccggtgc agatgagtgc ctttcgctgc cttttcaccc
     3181 tcgcgaattg cttgcgcgta tcaaggcggt cttacgaaga acagagcggc aggtggccag
     3241 tccgcatcgt ctcttacgat gcggctctct cgaacttgat tctgaacaga gggaagtacg
     3301 cgtcgcaggg caagtgattg gggtgacgag cttggagtac gagttactgt atgtcctgct
     3361 gcgaactcct ggccgcacct tctcacgcgc gcaattgctc gaactcatcc ctggtttcca
     3421 gcatcgctcc ccttttgatc gcgccgtgga tattcatatc agcaatctgc ggcgcaagct
     3481 gagccaggta atgggaacgg acgcgctcat tgaaaccgtg cgtggggtag gctatcgctt
     3541 gagtgtgccg acaactgaaa ctgttgccga accagcgtca cctggtccag tacctacgca
     3601 gctagcgctg gcagcttttg aacgcgcccc cgtccctctt ttagtgctcg cggctgatcg
     3661 cacggtggtc ctttataatg aggcggccag gcatctctgt ggctggtctg ctgaccaggt
     3721 ggtcggtcag gcgaaatgct acagcctgct ctcctgccac ctggcaaacg gcaccatgct
     3781 ctgtcgcgaa cagtgtgtga tgaacgccat cacgctcaat cggctgagcg atcaaacggc
     3841 ctgctactcc atcacgctca aagacgggcg cgaagtgccg gtgacggcgc attatagcag
     3901 tctcgacgag cctggaatgg atggaggcta caccatgctg gcgctcgcgc ctgacctcgc
     3961 cggaacatac cttccttaaa atgcgtaaac aggaagaagg ttatcacgct ttctgcttgt
     4021 agatactcag acggcaatac gaaagcgacc aggggctatt tcatgcttat cctcgcct
//
LOCUS       DASZAK010000599         1783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_49558, whole genome shotgun sequence.
ACCESSION   DASZAK010000599 DASZAK010000000
VERSION     DASZAK010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49558"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /locus_tag="VGT44_10535"
     CDS             complement(<1..1500)
                     /locus_tag="VGT44_10535"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HEV2581275.1"
                     /translation="MTSANTTIATTTIPDTIASIPHGKYHIWTVGCQMNIADSQRIQT
                     MLDEMGWEQSSMEQANLVILNTCSVRKAPEEKAHNQLAMLEHAKARRDDLLVAMMGCM
                     IGNQKTIDELSKRYPHIDLFMKVEQADILPRFLEERWTPISGAGCLDIEYAPPESGEP
                     FPVEPLERLLPTFATSTERPGKRTVLPMAITPKPGERIAHYPTKIEPAQVSPTAWLPV
                     VLGCNKVCTYCIVPYRRGRERSRPIDELVTEAVSLVGKGAKELTLLGQTVEAYGLDLP
                     GKPDLAELMRQLSEIDGLQRIRFMTSYPRHMNDSMIERMAALPKVCEHVNIPVQSGDD
                     EVLRRMKRGYTLEEYRDRIARLRTLWPGVSLSTDIIVGFCGESEQEFQHTLDLLEEIR
                     FDVVHVAAYSVRPGTVAARWEDDIPLAEKKRRLHALEETQARIALELNQPLIGDIEEV
                     LVEEANFTHGRQQWKGRNRINKWVFFPQPVEEHDEPRGDALRRGIQPGDL"
BASE COUNT          330 a          529 c          526 g          398 t
ORIGIN      
        1 caggtcgcca ggttgaatgc cgcgccgcag agcatccccg cgcggctcat catgttcttc
       61 caccggctgc ggaaaaaaca cccacttgtt gatgcggttg cgccccttcc actgctgtcg
      121 tccatgcgta aaattagcct cttccaccag cacctcttcg atatcgccga tgagcggctg
      181 gttcagttcc agcgcgatgc gtgcctgcgt ctcttccagg gcatgcaggc ggcgtttctt
      241 ctcagccagc ggaatatcat cctcccaacg cgccgcgact gtgccgggcc gcacagagta
      301 ggccgcgaca tgcacgacat cgaagcggat ttcttcgagc aggtcgagcg tgtgctggaa
      361 ttcctgctcg ctttcgccgc agaagcctac gatgatatcg gtcgagagcg agacgcccgg
      421 ccacagggtg cgcagccgcg cgatgcggtc gcgatactct tccagcgtat aaccgcgctt
      481 catacgccgc aggacttcgt catcgcccga ctgcaccgga atattgacat gctcgcagac
      541 ctttggcaga gcggccatgc gctcgatcat gctgtcgttc atatggcgcg gatacgaggt
      601 catgaagcga atgcgctgca agccatcgat ctcgctcaac tggcgcatca actcggccag
      661 gtccggcttg cccggcaggt ccagcccata cgcctcgacg gtctggccga gcagggtcag
      721 ttccttcgcg cccttgccga ccagcgagac ggcctctgtc accagttcgt cgatgggacg
      781 gctgcgctcg cggccccggc gatacggcac gatgcagtag gtgcagacct tgttgcagcc
      841 cagcacaacg ggcagccacg ctgtcgggct gacctgcgcc ggttcaattt tcgtggggta
      901 gtgggcgatg cgctcgccgg gtttgggcgt aatggccatc ggcagtacgg tgcgcttgcc
      961 aggccgctcg gttgacgtag cgaaggttgg caggaggcgc tcgagcggct caactggaaa
     1021 gggttcgccg gactcaggcg gggcatattc gatatccaga cagcccgcgc cggagatggg
     1081 cgtccagcgc tcttcaagga agcgcggcaa aatatcggcc tgctcgacct tcatgaacag
     1141 gtcgatatgc gggtagcgtt tgctcagttc atcgatggtc ttctggttgc cgatcatgca
     1201 acccatcatg gcgaccagca gatcatcgcg cctggctttg gcatgttcga gcatggcaag
     1261 ctggttgtgc gccttctctt ccggcgcttt acgcacgctg catgtattca ggatcaccag
     1321 gttggcctgc tccatactcg actgctccca ccccatctcg tccagcatgg tctggatgcg
     1381 ctgtgaatcc gcgatgttca tttgacaccc gacggtccag atatggtatt ttccatgcgg
     1441 aatggaagca atagtatccg gtatagtcgt tgtggcaatt gtcgtatttg ccgaagtcat
     1501 gccgttgccc ctcgagatat ctatggtcaa tgctctctta atgctgatat ggtgggagtc
     1561 acctgttcta agttggacat tatacactat gcggggcgct gtgtcacgtt tttttaaaga
     1621 gcagggtgcg gttccttttt tgcacagaag agtactcggc catcgccctg gctacacact
     1681 ggattagcca gcgatatgca gtacgatacg tccgcgtcca tgaccgctct ggctcaattc
     1741 gtgggcgaga ctcgcctcgt gcaaggggaa gatgctggaa agg
//
LOCUS       DASZAK010000600         2779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_298447, whole genome shotgun sequence.
ACCESSION   DASZAK010000600 DASZAK010000000
VERSION     DASZAK010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298447"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VGT44_10540"
     CDS             <1..638
                     /locus_tag="VGT44_10540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2581276.1"
                     /translation="ALTGIGGDLNDQHREFVQRARSGSEHLYALVEDLLLVSRADAGQ
                     LRLSRAALQLDEVVANSVEELELTAKDSGVTIEVDIPDDLPQLYADSVRLQQVLRNLL
                     SNAIHYTPSGGRVTISARSIDHEHNEQAGEQSAISIEVADTGSGISADFHERIFERFF
                     QVPIDISGRAGGQGLGLAIVKMIVELHGGHVSIRSVPGEGSAFTFTIPATL"
     gene            780..1025
                     /locus_tag="VGT44_10545"
     CDS             780..1025
                     /locus_tag="VGT44_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581277.1"
                     /translation="MGMLRIMSRKGDERVAWDLKKVETNDPEATAAIREAERIFNQEQ
                     AHGATAFKVESGKPVERIETFDRTAEQIVMVPRVVGG"
     gene            1057..1509
                     /locus_tag="VGT44_10550"
     CDS             1057..1509
                     /locus_tag="VGT44_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581278.1"
                     /translation="MWETLSLFLGIWLLFLLLAIYYLSQAPDGRVGRFRESVSEHLSA
                     ESRAKVLLREMLTENQFQQLIKFGYLEVQSPTIDNRVYRIPGSGGLVKVYERGCAVME
                     LCLQPAEPLPDGDVVVMHKLMIEANEQEYLQKANHFAPGIISLRGQHL"
     gene            1744..>2779
                     /locus_tag="VGT44_10555"
     CDS             1744..>2779
                     /locus_tag="VGT44_10555"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2581279.1"
                     /translation="MKCPYCNKDNADGVSYCSNCGKFIGSGPAPNTPATNGGSSVHSR
                     ALTAGSTLQGGRYVVKKVLGEGGMGAALLAIDKRLDDKQVVIKELISDSSDPEKFKED
                     VRNFKLEMRTLARLEHPLIPAVTDNFEENAHYYMVQEYVAGENLEERLDHLQAPMKER
                     DVLTYASQVLDVLEYLAQETPPILHRDIKPANIIISDKDKRAHLVDFGIARQNIARKQ
                     TSALGTPGYAPPEQYQGKADPRSDLYALGATLHHLLSNRDPRNFPPFVYPPLRSINPQ
                     VTAETERVITKSVNNDVTLRYQSATAMKHDIDDILLKRFGMSGTISSYTLGATGSTAI
                     VPPATANYNAV"
BASE COUNT          666 a          801 c          760 g          552 t
ORIGIN      
        1 tcgcgctgac cggcatcggc ggtgatctca acgatcagca tcgcgaattc gtgcagcgcg
       61 cccgcagtgg cagcgagcat ctctacgcgc tggtcgaaga cctgctgctc gtctcgcgcg
      121 cggacgccgg tcaactgcgc ctgagccgcg ccgcgctgca actcgacgag gttgtcgcta
      181 actccgttga agaattggaa ctgaccgcga aggatagcgg cgtcaccatc gaggtcgata
      241 ttcccgatga cctgccgcag ctctacgccg atagtgtgcg cctgcaacag gtattgcgca
      301 acctgctgag caacgccatt cactataccc cctccggtgg ccgcgtcact atcagcgcgc
      361 gttccatcga tcacgaacac aacgaacagg caggcgaaca atccgccata tccatcgagg
      421 ttgcggacac cggcagcggc atcagcgccg attttcacga gcgcattttt gagcgcttct
      481 tccaggtgcc gatagacatt tcaggccggg ctggcggcca ggggctggga ctggcgatcg
      541 tcaaaatgat cgtcgaattg cacggcggac acgtgtctat acgtagtgta ccaggcgagg
      601 gaagcgcctt cacattcacc atcccggcga cattgtgatg agttgccctt gacggaacgg
      661 taaaaaccat gctatgctga ttttatgttt gaagggaagg gtgtgcctgc gcacacgcat
      721 gaaagtacga gacacagggt cgaagcgggc gcaagctcgc acagcaaagg agacagacca
      781 tgggaatgtt gcggattatg tcccgtaagg gcgacgagcg agttgcatgg gatctcaaaa
      841 aggtagagac caatgatccc gaagcgactg ctgcaatccg cgaagccgag cgcatcttca
      901 accaggaaca ggcgcacggc gcaacggcat ttaaagtaga gtctggcaaa cctgtagagc
      961 gtattgagac ctttgaccgc acagccgagc agattgtgat ggttcctcgc gtcgtcggtg
     1021 gctgatctga tgagcggcaa aggagcatga gaggcgatgt gggaaaccct tagcctgttt
     1081 cttggtatct ggctgctgtt cttgcttcta gcaatctatt acctgtcgca ggcccccgat
     1141 gggcgcgttg gacgattccg cgaatcggtc agcgaacatc tctcggctga aagccgcgcg
     1201 aaagtcctgc tgcgggagat gctaacagag aaccagtttc aacaactgat aaagtttgga
     1261 tatctcgaag tccaaagccc tactattgat aatcgcgtat accgcatacc cggctctggc
     1321 ggcctggtga aggtctatga gcgcggctgc gcagtcatgg aactctgctt gcagccagcg
     1381 gaaccactgc ccgacggcga tgtcgtggtg atgcataaac tgatgatcga ggcgaatgaa
     1441 caggaatacc tgcaaaaggc caaccatttc gctcctggta tcatctctct gcgcggccag
     1501 catctgtaaa acttccacga ttgtcaggat cgccgtcggc ttgacggcga tcttgatttt
     1561 ttgctttcat ctcattcctc gctgaacttt cctcaccata ttacgtatat atttcttgtg
     1621 atacctgttc catgttacca ctcgactgaa tactcgaatc caggtataat cactgctaag
     1681 tagtactcat gtccgataga gaccagctat tatcaaaaca atcatagaga aggtaggcac
     1741 gctatgaaat gtccctactg taacaaagac aacgcggatg gcgttagcta ttgtagcaac
     1801 tgtggcaagt ttatcggttc gggtcccgcc cccaacactc cagccacgaa tggcggcagc
     1861 agcgtccatt cgcgtgcgct gacggcaggc tctaccttgc aagggggccg ctacgtcgtg
     1921 aaaaaggtgc tgggcgaggg cggcatgggc gcggcgctgc tggccatcga taaacgcctg
     1981 gacgataagc aggtcgtcat taaagagttg atctccgata gcagcgaccc ggagaaattt
     2041 aaagaggacg tgcgcaactt caagctcgaa atgcgcacgc tggcacgtct cgaacatcca
     2101 ctcattccag ccgtcaccga caacttcgag gaaaatgccc actattacat ggtgcaggag
     2161 tatgtcgcgg gagaaaacct ggaagagcgc ctggaccacc tgcaagcgcc gatgaaagag
     2221 cgcgacgtgc tgacgtatgc gtcccaggta ctcgacgtgc tggagtatct ggcacaggaa
     2281 acgccgccga tcctgcatcg cgatatcaaa ccggccaaca tcattatcag cgacaaggac
     2341 aaacgcgccc acctcgtcga tttcggcatc gcgcgccaga atatagcgcg caaacagacc
     2401 tcagcgctcg gcacacccgg ctacgcgccc cccgaacagt accagggcaa agccgacccg
     2461 cgctccgacc tgtacgcgct gggcgcgacg ctgcaccacc tgctcagcaa ccgcgacccg
     2521 cgcaacttcc caccgtttgt gtatccgccg ctgcgcagca tcaacccgca agtcacagcg
     2581 gagaccgagc gagtgattac aaagtcggtc aataacgatg tcaccctgcg ctaccagagc
     2641 gcgacggcca tgaaacacga cattgatgat atcctgctca aacgctttgg catgtcggga
     2701 acgatcagca gttatacact cggcgcaacc ggctcaacag ctatcgttcc gcctgccaca
     2761 gctaattaca acgctgtca
//
LOCUS       DASZAK010000601         1834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_298451, whole genome shotgun sequence.
ACCESSION   DASZAK010000601 DASZAK010000000
VERSION     DASZAK010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298451"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VGT44_10560"
     CDS             <1..604
                     /locus_tag="VGT44_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEV2581280.1"
                     /translation="VQPLRSILLDLTPDEDALLASMKEKWRYNVRLAGRKGVMVHMAQ
                     TTQDIQGWYQILRVTGQRDGFGIHTLDYYLRAWQLFAPRNLAQLFLAEHDGRLLAGIF
                     VGCMARQAIYLYGASSNEQRQLMPNYLLQWEAMRWAKSQGAQSYDFWGIPDSDDPGEA
                     LAGVYRFKSGWGGQVVRFAGNYEYVCRPVMMRLARRFVRM"
     gene            772..>1834
                     /locus_tag="VGT44_10565"
     CDS             772..>1834
                     /locus_tag="VGT44_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581281.1"
                     /translation="MYRDRQKFVYRSRGLARPRLVVMLGLVFLAGAAAMFVRQGMLDA
                     RSAPQQGQSASAQTQATGVCQQQGAFRYFVLKQASGFALARAAVGGIGQPLGAPQPVA
                     NFGNDFGQSESDAVFSMQLSPDGCYLAIDGASDHIEQVWVYDIRHAALALLPANVTGA
                     FLNWLPGKGSGHAFLYRPMLPLGPGAPLVNGAWNPGLWIVDAATGQYRNIDIHMSSAF
                     LVDAAASPDGTHIVYSISFGLGLGSDIWLMNGDGSHQTHLFSLAGGGQSIAGMFTWSP
                     DGSQIAYEQLSDSSVPFQPAGLWIMNSSGAASRQVARTDGGHGYRPTWSPDSRYIAYI
                     ARTNDGDRMADYNAQSLQCG"
BASE COUNT          335 a          511 c          595 g          393 t
ORIGIN      
        1 tgtacagcca ttacgcagca ttctgctcga tctcacccct gacgaagatg cgctgctggc
       61 cagcatgaag gagaaatggc gctacaatgt gcggctggcc gggcgcaagg gcgtcatggt
      121 gcatatggcg cagacgaccc aggatattca gggctggtat caaatcctgc gggtgacggg
      181 ccagcgtgac ggcttcggta ttcatacact cgactattac ctgcgcgcct ggcagctctt
      241 tgcgccgcgc aacctggcgc aactgttcct ggcggaacac gatggccgtt tgctcgcggg
      301 catttttgtg ggctgcatgg ctcgacaggc gatctatctt tacggcgcat cctccaacga
      361 gcagcgccag ctcatgccca actacctgtt gcaatgggag gcgatgcgct gggcgaaaag
      421 ccagggcgcg caaagctatg atttctgggg tattcccgat agcgacgatc ccggcgaggc
      481 cctggccggt gtgtatcgtt tcaaaagtgg ttggggcggt caagtcgtgc gctttgccgg
      541 caattacgaa tatgtctgtc gcccggttat gatgcggtta gcgcgccggt ttgtgcgcat
      601 gtgaggttga cagggacgcg ggcgacgcca gggtgtctgt gcctcaaccg ggaacgcggg
      661 cgacgcaagc gtccacaccc cgcccgtcgt ccacacccgc ccctacagca acgactccat
      721 gtaccgatag ataggaatga aacgatctat ctatctggaa tggagatgtg tatgtaccgg
      781 gatcggcaga aatttgtgta tcgttcgcgt ggcctggcgc gtccgcgact ggtagttatg
      841 ctggggctgg tgtttctggc gggcgcagcg gcaatgtttg tgcggcaggg gatgttggat
      901 gcgcgcagcg cgccacagca ggggcagtcc gcgagcgcgc aaactcaggc gacgggcgta
      961 tgccagcagc agggagcatt tcgctatttt gtcttgaagc aggcgagcgg ctttgcgttg
     1021 gcacgcgccg cagttggagg gatcggccag ccacttggcg cgccgcagcc ggtggcgaat
     1081 tttgggaatg attttgggca gtcggaatct gatgctgtat tttcaatgca actctcgccc
     1141 gatggatgtt atcttgccat tgacggagcg agcgaccata tcgaacaggt gtgggtctac
     1201 gatatccggc atgcggcgct ggcgctgctt ccggcgaatg tcacaggcgc ctttctgaac
     1261 tggctgcccg gcaaaggcag cggccatgcc tttttgtatc gccccatgct gccgcttggc
     1321 cctggcgcgc cgctggtgaa tggagcctgg aatccaggtt tgtggatcgt ggacgcggcg
     1381 acgggacagt atcgaaacat cgatatccat atgtcatcgg ccttcctggt tgacgcggcc
     1441 gcgtcgcctg atggtacgca tatcgtgtat tcaatctcgt ttgggctggg tctgggcagc
     1501 gatatttggc tgatgaatgg tgatggcagt caccagaccc acctgttttc cctggcgggc
     1561 ggcgggcagt cgattgccgg catgttcacc tggtcgcccg atggctcaca gatcgcctac
     1621 gagcagttga gcgatagctc ggttcccttc caaccagccg ggttgtggat catgaacagc
     1681 agcggagcgg cctcgcgcca ggtggccaga accgacggtg ggcatggcta ccggccgacc
     1741 tggtcgcccg atagccgcta cattgcctac atcgcgcgca ccaacgacgg cgaccgcatg
     1801 gccgattaca acgctcagtc cttgcaatgt ggca
//
LOCUS       DASZAK010000602         2202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_547432, whole genome shotgun sequence.
ACCESSION   DASZAK010000602 DASZAK010000000
VERSION     DASZAK010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547432"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VGT44_10570"
     CDS             complement(<1..1112)
                     /locus_tag="VGT44_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581282.1"
                     /translation="MHPARHSKRSLILSLLGLVALVGTATFSIGLERHSSQAHAAASS
                     SRLRLASIPTNIDVSQRLTNESEETVAVNPTNPKNIVIVTNVDHPAAGMFEGVSFDGG
                     ATWTTKLIGNNDNLGDACCDPSLSFDNYGNLFMTYLYNVELEVPIALSTDGGLNFKLI
                     ANIAKPPKQSLATSSERRGLFRFVDQPTITAAEGEVWLVFNGGGPMVATGAPVTGVGQ
                     VGNFIPLEVIPGTNNCTYGDVSIGPTGQVMQVCTLTESGQGGGKLFVNVDPDGLGPAG
                     FGTRVFVAETHVGGFDFIPAQPDRSVDAEPGLAWDRTGGPHNGRVYLVSTREHPNESN
                     NMDIYVRFSDDNGATWSGAVRVNDDNTKNSQFLPKIS"
     gene            1546..1833
                     /locus_tag="VGT44_10575"
     CDS             1546..1833
                     /locus_tag="VGT44_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581283.1"
                     /translation="MKRFIVVSAILVILVAGLAVSSFTRVFAANPGTQGQPNQSCQSF
                     FGPGTPLVPNGFNTNGFATAGANYAGSQPQNSNNPNSVSQYDVACFQSTQH"
     gene            complement(1946..>2202)
                     /locus_tag="VGT44_10580"
     CDS             complement(1946..>2202)
                     /locus_tag="VGT44_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581284.1"
                     /translation="RDQNLLYVAGLHGIWVINTNTLRVQGEYLKQQSFTGLAMSGDGY
                     TLYGVDPATGITLLNASSGEMRSVIKGVIQAPWGVEWLAQ"
BASE COUNT          476 a          630 c          596 g          500 t
ORIGIN      
        1 gagatcttcg gcaggaactg gctgttcttc gtgttgtcgt cgttcacccg caccgcgccg
       61 ctccacgtgg cgccgttgtc atccgagaag cggacgtaga tatccatatt gttgctctcg
      121 ttgggatgct cgcgcgtgga taccaggtac acgcgcccat tgtgtgggcc accggtccga
      181 tcccaggcaa ggccaggctc cgcgtcgacc gaacggtcgg gctgcgccgg aatgaagtca
      241 aatccaccga cgtgtgtttc agccacgaac acccgcgtgc caaagccagc cgggcccagc
      301 ccatcgggat caacgttcac aaagagcttg ccaccgccct gcccgctctc ggtgagcgtg
      361 cagacctgca tgacttgacc agtcggacct atcgatacgt caccgtacgt acagttattt
      421 gtgccaggga tgacttcgag gggaatgaag ttgccgacct gcccgacccc cgttacgggc
      481 gcgcccgtgg ccaccatcgg accgccgcca ttgaatacca gccagacctc gccctcggca
      541 gcggtgatcg tgggctggtc gacaaacctg aacaggccgc gtcgctcgct gctcgtcgcc
      601 aggctttgct ttggtggctt cgcgatattc gcaatgagtt tgaagtttag gccgccgtcg
      661 gtgctgaggg cgatggggac ctcgagctcg acattataaa ggtaggtcat gaaaaggttg
      721 ccgtagttgt cgaatgacag gctggggtcg caacacgcgt ctcccaggtt gtcgttgttc
      781 ccgatcaact tcgtggtcca ggtcgcgccg ccatcgaagc tcacgccctc gaacattccg
      841 gctgccggat ggtccacgtt cgtgacgatc acgatattct tcgggttggt cggattgacc
      901 gccaccgtct cctcggactc gttggtcaac cgctggctga catcgatgtt cgtaggtatg
      961 gaggcgagcc gcaatcgact gctggatgca gccgcgtggg cctggctcga atgacgttct
     1021 aagcctatgc tgaatgtcgc tgtccccacc agggcaacca ggccgagcag ggagagaatg
     1081 agactacgtt tggagtgtcg agcgggatgc atagaaaacc ctcactttgc tattctcttc
     1141 aatagagcgc acgtctctcg cacgtgcagg gcagagcgtt gagcaggcct gctgccgcta
     1201 aaacaagagg caacaagaat cacgtctatg gtaggtgggt gggtaaccag cgtcaagggc
     1261 aggagggtag tgattgagtt aaagacgggt taagattgta gttggtgggt gcaggtttct
     1321 acattcagcg ttgttttcgg aacgaatgca ttcaaagaac atcactaaaa cttgaaatca
     1381 taaccggttc ttaaccagac cacaacccct ttgctcttgc ctctacacac agatgctcct
     1441 atgattcctc cggttaattg cattgatctt atcaaagtac ttcgctctca aaaatgtaat
     1501 agacaaggag ccgttgacaa actgagcctg gaaaggagct taattatgaa gcgttttatc
     1561 gtcgttagcg ctattctcgt catcctggtg gctggcctgg ccgtctcttc atttacaagg
     1621 gtgtttgccg cgaaccccgg aacacaggga caaccgaatc agagttgcca gtcgtttttt
     1681 ggccctggga cacctcttgt cccaaacgga ttcaatacca acggtttcgc caccgcgggc
     1741 gccaactatg caggcagcca gccgcaaaac tcgaacaatc ccaacagcgt atcccagtac
     1801 gacgtagcgt gcttccagag tacccagcac tagcatgagt ttgcttgtct accttccctg
     1861 atctcaactt cagtaaaggt gtgcacagca atcatggaag tcatcaaaat gcctggaaag
     1921 agcgatcgct ctttccaggc attttctact gagccaacca ctcgacgccc cagggcgcct
     1981 ggattactcc cttgattacc gagcgcattt ctccactgct agcattcagc agggtaatgc
     2041 cggtcgcggg gtcaacacca tagagcgtgt atccatcgcc actcatggcc agcccggtaa
     2101 agctttgctg ttttagatac tcgccttgca cccgaagcgt attcgtgttg atcacccaga
     2161 tgccgtgtag tcccgccaca tacagcaagt tctgatctcg cg
//
LOCUS       DASZAK010000603         5387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_583802, whole genome shotgun sequence.
ACCESSION   DASZAK010000603 DASZAK010000000
VERSION     DASZAK010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583802"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /locus_tag="VGT44_10585"
     CDS             <1..676
                     /locus_tag="VGT44_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2581285.1"
                     /translation="DLFVSLRSDDTVRVVILTGAGDNFCSGGDVHEIIGALLQRDMQG
                     HLEFTRMTGAVVQNMRQLDKPIIAALNGMTAGAGAVLALACDLRIASERARFAFLFTK
                     VGLTGADMGAGFLLPRVIGLGRASELLLLGDTIDVVTAERYGLVNRVVAHNELLPAAR
                     QWAKRLADGPTLAISMTKHMLNNELNIDLASAIEAEAQAQALMLMGDAHRKFYESFQT
                     RQKRSG"
     gene            717..1118
                     /locus_tag="VGT44_10590"
     CDS             717..1118
                     /locus_tag="VGT44_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEV2581286.1"
                     /translation="MGKDIINPSSLAKPVGFSHGIMTTGGHLLFLAGQTGSDAEGRIT
                     EPSNVVAQYKQIMRNLQTVVLSAGGTMEDITKLTIFVRDRDDYMAHLKALGEVHRAFF
                     GSYYPAMALFEISRFFQDDALIEIEGMAVIE"
     gene            1131..2288
                     /locus_tag="VGT44_10595"
     CDS             1131..2288
                     /locus_tag="VGT44_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2581287.1"
                     /translation="MDFELTPAQQRIQAEARRFAEHEVAPLAREADEQGIFPLHLVKR
                     MGELGFLGGPIDPAYGGSGMDSVSYSLLCEELGRADSSVRGFLTVHASLVSLCIQAWG
                     SEGQKRSYLPRLARGEWIGCYALTEPNAGSDAASMETTATEQGDAYLLNGEKIWITNG
                     TSARIAIVFASMDLTLRHKGICAFLVETDTPGFQRTPMPGKELGHRASEHAHITLRDC
                     RVPKSALLGKPGEGFKVAMSALDRGRLGVAAGAVGVAQACLDACVAFTTQRRQFGQRI
                     ADFEMVQATLADMAADVEAARLLVYRASWNIDQGLPATKATSIAKLFATEAAMRAASQ
                     AVLLHGNRGYSNEYPVERYYRDIKGLQIYEGTSHIQRVIIARELVGRDRQW"
     gene            2387..3997
                     /locus_tag="VGT44_10600"
     CDS             2387..3997
                     /locus_tag="VGT44_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2581288.1"
                     /translation="MNAYSAHIDTFARDNLPPRDEWPDLIFTIPEVQYPDRLNCMVEL
                     LDKKVRQGDGDRPAIFGADGVLTYAQMQVQVDKVAHVLSHDMGLVPGNRVLLRGGNNA
                     MMAVCWCAVAKAGLIVVATMPLLREKELVDIIEKAQVSAAICDQRLDAELLAAQKRCP
                     VLQQIVYFNNASPEGLEARMAAWSEPFSAADTAAEDTVLIAFTSGSTGQPKATMHFHR
                     DILAVCDCFPKSIVGMTQDDICIGTPPLAFTFGLGALLIFSMREGAATVLLEQLTPEK
                     LLAAVGNYRATIIWSSPVFYRQMAEIAGRFDLSTLQQCVSAGEALPVSTRAMWLDATG
                     IDIIDGIGSTEMLHIFIASAGTDIRPGATGKVVPGYQACILDEDGQQLPPDTVGRLAV
                     KGPTGCRYLADERQRTYVQHGWNLTGDAYLMDADNYFWFQARTDDMIITAGYNVAGPE
                     VEGALLMHPAVAECAVVGTPDPERGTIIKAFVVLRPGYVGDEALVKTLQDFVKQHIAP
                     YKYPRAIAFSEALPRAGTGKIQRFRLRQ"
     gene            complement(4063..4680)
                     /locus_tag="VGT44_10605"
     CDS             complement(4063..4680)
                     /locus_tag="VGT44_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2581289.1"
                     /translation="MHDLLDSVAETEVVGEATTGAEAITQAKALQPDVILMDIKMPGI
                     NGIEATREIVAASPHISILMVTMLEDDESVFAAMRAGARGYVLKGANQAEILRAIRAV
                     ASGEAIFSPGIARRVLGFFAAARPSVPPRVFPELSERETELLGLMAQGHSNQVIADQL
                     GLTIKTVRNHVSNIFSKLQVADRAQAVLRAREAGFGYGQTEDGSN"
     gene            complement(4578..5240)
                     /locus_tag="VGT44_10610"
     CDS             complement(4578..5240)
                     /locus_tag="VGT44_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581290.1"
                     /translation="MPKERKAAVWESNWDNWPFPLSAVVLFGLLGVLGGSQTYRYRRL
                     SNPVQRQQTKWVVFALIVTFMGIAGDVVVFDLIPQLSPALSPPDLLSHVIGPFAWNIV
                     PVVVPLAIGIAILRYRLWDIDIIINRTLVYGVLTVSIVTLYVLVVVGLGALFQAQANL
                     AIALLATGLIAVLFHPLRRIIRSFARACTTCWTRSRRPRWSARPPPEQRPSHRRRRCN
                     RT"
BASE COUNT         1099 a         1598 c         1624 g         1066 t
ORIGIN      
        1 cgacctgttc gtctccctgc gcagtgacga cacagtgcga gttgtgattt tgacgggggc
       61 gggcgacaac ttttgttcgg gcggagacgt gcatgagatc atcggcgcat tattgcagcg
      121 cgatatgcaa ggccacctgg agtttacgcg catgacgggg gctgtcgtgc aaaacatgcg
      181 ccagctcgac aagcccatca tcgcggcgct caacggcatg acggcaggcg cgggcgcggt
      241 gctggcgctg gcttgcgacc tgcgcatcgc ctcggaacgc gcgcgtttcg ccttcctctt
      301 cacgaaggtg ggcttgaccg gcgcggacat gggcgcgggc tttctgctgc cgcgcgtgat
      361 cggcctgggg cgcgcctcag aactgctgct gctcggtgac actatcgatg tcgtgacggc
      421 ggaacgctat ggcctggtca accgcgtggt tgcccacaat gaactactgc cagccgcgcg
      481 gcagtgggca aaacgcctcg cagatggtcc gaccctggcc atctctatga ccaagcacat
      541 gttgaacaac gaactcaaca tagacctggc ttccgctatt gaggccgaag cacaggcgca
      601 ggcgctcatg ctgatgggcg acgcacaccg taagttctac gaatcgtttc aaaccagaca
      661 gaaaagaagc gggtaatact tgagatagaa tgatctgaaa aagaggaaga agagctatgg
      721 gaaaagacat catcaaccca tcctcgctgg ctaaaccggt cggtttcagt cacggcatca
      781 tgaccacggg tggacacctg ctcttcctgg ccgggcagac tggcagcgac gccgaaggac
      841 gcatcaccga gccgagcaac gtggtagcac agtataagca aatcatgcgc aatctccaga
      901 cggtcgtcct ctccgctggt ggtacgatgg aggacatcac caaactcact atttttgtgc
      961 gcgaccgtga cgactacatg gcgcatctca aggcgcttgg ggaggtgcat cgcgccttct
     1021 tcggctcata ctacccggcg atggccctgt tcgagattag ccgtttcttc caggatgatg
     1081 cgttgatcga aatagaaggc atggccgtga tcgagtaaaa ggacagagca atggactttg
     1141 aactgacacc agcgcagcag cgcatccagg ccgaggcgcg ccgcttcgcc gagcatgagg
     1201 tcgcgccgct tgcgcgagaa gccgatgagc agggcatctt cccgctgcac ctggttaaac
     1261 gtatgggcga gctgggattc ctgggagggc cgattgatcc tgcctatggc ggctccggca
     1321 tggattccgt cagttactcg ctgctttgtg aagaacttgg tcgcgccgat tcgtccgtgc
     1381 gtggtttcct gaccgtccac gccagccttg tctcgttgtg tatccaggcc tggggcagcg
     1441 aggggcagaa gcgctcctac ctgccgcgcc tggcaagggg cgaatggatc ggctgctacg
     1501 ctctcactga gccaaatgcc ggttccgacg ccgccagcat ggagacaacc gcaactgaac
     1561 agggcgatgc ctacttactc aacggcgaga aaatctggat caccaacggc acgagcgctc
     1621 ggatcgccat cgtcttcgcc agcatggatc tcacgttgcg acacaagggt atctgcgctt
     1681 tcctggtcga gacggatacg cccggctttc agcgcacacc catgccgggc aaagaactcg
     1741 gccatcgcgc ctcggaacac gcgcatatca ccctccgcga ttgtcgcgtg cctaagagcg
     1801 cgctgctggg caaaccgggc gaaggcttca aggtcgccat gtccgcgctg gatcgcggtc
     1861 ggctgggcgt tgcggcaggc gcggtcggtg tggcgcaggc gtgcctggat gcttgcgtcg
     1921 ccttcacgac gcagcgccgc cagttcggcc agcgtatcgc cgatttcgag atggtccagg
     1981 caaccctggc cgacatggcc gccgatgtcg aagcggcgcg cctgctcgtt tatcgcgcgt
     2041 catggaacat cgaccaggga ctgcctgcca cgaaagctac ttcgatagcg aagctcttcg
     2101 cgaccgaggc tgctatgcgc gcggcatccc aggcggtgct gctgcacggt aatcgcggct
     2161 attccaacga atacccggtg gaacgctact accgtgacat taaaggcttg cagatttacg
     2221 aggggaccag ccacatccag cgcgtcatca tagcccgcga actggttgga cgggataggc
     2281 aatggtaaat gacctgtagg ggtgcaattc atagcgccct cgtccccacc gaagcggcct
     2341 ggcaagcggg tgcaatgaat tgtgccccta caaaggagta gaaaaaatga atgcatattc
     2401 agcccacatc gatacctttg cgcgcgacaa tttgcccccg cgcgacgagt ggcccgacct
     2461 gatctttacc atccccgaag tacagtatcc tgaccgtctg aattgcatgg tcgaactgct
     2521 ggacaaaaaa gtacgccagg gggacggaga ccgtccggcg attttcggcg ctgacggcgt
     2581 cctgacctac gcacagatgc aggtgcaggt agataaagtg gcccacgtgt tgtcccatga
     2641 tatgggactc gttcccggca accgggtgct gctgcgcggg ggcaataatg ccatgatggc
     2701 cgtttgctgg tgcgccgtcg cgaaggccgg gttgatcgtg gtggcaacca tgccgctgct
     2761 gcgcgaaaaa gaactggtgg atatcatcga aaaggcgcag gtgagcgccg ccatctgcga
     2821 tcagcggctc gatgccgaac tgctcgccgc ccaaaagcgc tgcccggtat tacagcagat
     2881 cgtctacttc aacaatgcct cacccgaagg gcttgaggcg cgcatggcag cctggagcga
     2941 gccgttcagc gccgcggaca ccgccgccga agatacggtt ctgatcgcct tcacctccgg
     3001 ctcgaccggc cagccgaagg caaccatgca ttttcatcgc gatatcctgg cggtctgcga
     3061 ttgttttccg aaatcgatcg tgggcatgac gcaagatgac atctgcattg gcacaccgcc
     3121 tctggccttc accttcggcc tgggtgcgct gctgatcttt tcgatgcgcg agggcgcggc
     3181 gacggtcctg ctagagcaat tgacccccga aaagctgctt gcggcagttg gcaattaccg
     3241 cgcaaccatt atctggtcat ccccggtctt ctatcgtcag atggcggaga ttgccggcag
     3301 attcgacctt tcgacgctgc aacagtgcgt ttctgcgggc gaggcgctgc cggtctcgac
     3361 gcgcgccatg tggcttgatg cgacgggcat tgatatcatc gatggcatcg gctcaaccga
     3421 gatgctgcat atcttcatcg cctcggctgg cacggatatc cgccccggcg ctacgggcaa
     3481 agtcgtgcca ggctaccagg catgtattct tgacgaggat ggtcaacagt tgccaccaga
     3541 taccgttggg cggctggcgg tgaagggacc caccggctgt cgctacctgg ccgacgagcg
     3601 ccagcgcacc tatgtacagc acggctggaa tctcaccggc gatgcctatt tgatggatgc
     3661 cgataactac ttctggttcc aggcgcgcac ggacgacatg attatcaccg caggctataa
     3721 cgttgctggg ccggaggtcg aaggcgcctt gctcatgcat cccgccgtag ccgagtgcgc
     3781 ggtggtaggc acacctgatc cagaacgcgg cacgatcatc aaggcctttg tggtcctcag
     3841 acccggatac gttggagatg aagccctggt caaaacgttg caggattttg tcaagcagca
     3901 catcgcgccc tacaagtatc cccgcgccat tgcattcagc gaggcgctgc cccgcgcagg
     3961 cacggggaag attcaacgtt tcaggctgcg ccagtagatt gctcctggac agaatgttag
     4021 tgcaggtcca agaaagctta tgtgtcaggc gggagaaatc tgttagttgg atccatcttc
     4081 tgtctgtcca tacccaaatc ctgcttcccg cgcccgcaga accgcttgcg cccgatcggc
     4141 tacttgcagc ttgctgaaaa tgttggagac atggttgcgc accgttttga tggtcagccc
     4201 caactgatct gcgattactt gattgctgtg cccctgcgcc atcagcccca gcagttccgt
     4261 ctcccgctcg ctcagttctg gaaagacccg tggtgggacg ctcggcctgg ccgccgcgaa
     4321 gaagccgagc acacgcctgg caatgccggg gctgaagatg gcctccccgc tggccaccgc
     4381 ccggatcgcc cgcaggattt ccgcctggtt tgcgcctttg agcacatagc cacgtgctcc
     4441 cgcacgcatg gcggcaaaga ccgactcgtc gtcttcgagc atggtcacca tcaggatgct
     4501 gatgtggggg ctggccgcca caatctcgcg ggtggcctcg atgccattga tgcccggcat
     4561 cttgatgtcc atcaggatca cgtccggttg cagcgccttc gcctgtgtga tggcctctgc
     4621 tccggtggtg gcctcgccga ccacctcggt ctccgcgacc gagtccagca ggtcgtgcag
     4681 gccctcgcga aagagcggat gatcctccgc aaggggtgaa acagcacggc gatcaagccc
     4741 gtcgccagca gcgcaatagc gaggttggcc tgcgcctgga acagtgcgcc aaggccgacc
     4801 acgaccagga cgtagagggt gacgatgctg actgtgagca caccgtacac cagggtgcga
     4861 ttgatgatga tgtcaatgtc ccacagccgg tagcgcagga tggcgatgcc aatggcaagg
     4921 gggacaacga cagggacgat gttccaggca aatgggccta ttacatgaga gagaaggtca
     4981 ggtgggctga gtgcggggga aagctgagga atcaagtcaa agacaacaac atcccctgct
     5041 attcccatga aggtaacgat cagggcgaag acgacccact tcgtctgctg ccgctgcacc
     5101 ggattggaca ggcgacggta gcggtaggtc tgcgatcctc caaggacccc cagcaacccg
     5161 aagagtacca ctgcactcag cggaaagggc cagttatccc agttgctctc ccacaccgct
     5221 gctttccgtt cctttggcat ccggttaccg ctgccttcct ttacgtcgtt gatagtgctg
     5281 caaatcctgt ttaatggtgg cttgatcgac ttcggcgtag agcaaggtgc tttgaatgtt
     5341 gcggttcatt tctcgcgagt gtaccatata acagaagtta tatggta
//
LOCUS       DASZAK010000604         2418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_86654, whole genome shotgun sequence.
ACCESSION   DASZAK010000604 DASZAK010000000
VERSION     DASZAK010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86654"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1102
                     /locus_tag="VGT44_10615"
     CDS             311..1102
                     /locus_tag="VGT44_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2581291.1"
                     /translation="MLVLQDISVTYHNIVPAVREVSIEVPDKTVVALLGANGAGKTTI
                     MRAISGLLSVHGASLARGSILFNGERIDHLNATQIVQRGIAQVLEGRRLFADLTIEEN
                     LRAGAIASRNPAATRKNFERVMDLFPVLRARLKSHAGYLSGGEQQMLAIGRGLMTSPK
                     LLLMDEPSLGLAPTIVQQIRGIIADINASGTAVLLVEQNAQMALSLAQHGYVLETGKV
                     VLDQSAEQLLKDEVVREFYLGLHEGSDRVNMATIRQRRPARRWDI"
     gene            1099..1902
                     /locus_tag="VGT44_10620"
     CDS             1099..1902
                     /locus_tag="VGT44_10620"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2581292.1"
                     /translation="MNATAVPPSSQPLLTVENISVRFAGVKALSDVSFDVKPGELFAI
                     IGPNGAGKTSLFNVLSRVYQPVEGKVTFDGHDLLRIKAHRIARSGIARTFQNLGQFPT
                     MTVLDYLLLGRHTRMRSGILSGGLYIGMTYREEQKNRAYCLRVLDLLGMKRLHNYPLG
                     SLPYGLQKRADVARALALQPKLLLLDEPVAGMGGEESADIAAAILDIKEQLGITQVLV
                     EHDMAIVMGIADRVLVLDFGRIIALDTPAGVQANPNVIKAYLGEEFEGK"
     gene            1904..>2418
                     /locus_tag="VGT44_10625"
     CDS             1904..>2418
                     /locus_tag="VGT44_10625"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2581293.1"
                     /translation="MSYFTQLAFAGVALGCIYALIGLGFSIIFKASEVINFAQGELLL
                     VGAYVVSSGMFEWHLPFLLALLLGIVVTVLIGVLFERFVLRRMIGRPVFSILMITIGL
                     DIVLRTVVEVRWGSNPIPAASPFNITSGIDRFGIHFGANDIWTIIVTIILCAALFVFF
                     RYTKYGLSMRAT"
BASE COUNT          486 a          730 c          660 g          542 t
ORIGIN      
        1 tcaactcatc acgttgctgt tgaagcaagg cggctgattt caaatagttt gtggctgaag
       61 tccaggcaac aggacgcatt tcttccatac gcgctgtcct tgcattgttc gagcaaacaa
      121 attcacgctt ccaatttgta gatgaaaaca ttgtatccca tgctgccacc tatttacaat
      181 taggttacac aggttctggc cgacctttca tagcatgtgt gtgtacttgc acacaaacga
      241 cgtatatact atagtatctt tcaatactcg tgcttccacg caaatatttt ttatcgggag
      301 aggacatttt atgctcgttc tccaggatat ctcggtaacg tatcacaaca tcgtccctgc
      361 cgtgcgggag gtgtcgatag aggtaccgga taaaacggtg gttgccctgc tcggcgcgaa
      421 cggcgcgggc aaaacgacca tcatgcgcgc catctccggc ctgctatccg tccacggggc
      481 cagcctcgcg cgcggcagta ttctctttaa cggcgagcgt atcgaccact tgaacgccac
      541 gcaaattgtg cagcgcggta tcgcccaggt gctggaaggc cgccgcctct tcgccgacct
      601 gaccatcgaa gaaaacctgc gcgcgggcgc gattgcctcg cgcaatcccg cagcaacacg
      661 caagaacttc gagcgcgtca tggacctgtt cccggtactg cgcgctcgtt tgaagagcca
      721 cgctggctat ctttcgggcg gcgaacagca aatgctggcg attgggcgcg ggctgatgac
      781 cagccctaaa ttgctgctca tggatgagcc atcgctgggc ctcgccccca cgattgtgca
      841 gcagattcgc ggcatcatcg ccgatatcaa cgccagcggc actgccgtgc tgctggtcga
      901 acaaaatgcg caaatggcgc tttcgctcgc ccaacacggc tacgtcctgg agaccggcaa
      961 ggtcgtgctg gatcagtccg cagaacagct tttaaaagac gaggttgtgc gcgaatttta
     1021 tttagggctg cacgaaggca gcgaccgcgt caacatggcg accattcgcc agcgccgacc
     1081 ggccaggagg tgggatatat gaacgccaca gccgtaccgc cttcgtcaca gccgctgctc
     1141 accgtcgaaa atatctccgt gcgtttcgcc ggtgtgaagg cactcagcga tgtctcgttc
     1201 gatgtaaagc cgggcgaatt gttcgccatc atcggaccca atggcgcggg caaaacctcg
     1261 ctcttcaacg tcctctcgcg cgtgtaccag ccggtggaag gcaaagtcac atttgatgga
     1321 cacgacttgc tgcgcatcaa ggcgcaccgc atcgcacgct ctggcatcgc acgcaccttt
     1381 cagaacctcg gacaattccc aaccatgacc gtgctggatt acctgctgct gggccgccat
     1441 acacgtatgc gcagcggcat cctttccggc ggcctctaca tcggtatgac ctatcgcgag
     1501 gagcagaaga accgcgccta ctgcctgcgc gtactcgatc tgctcggcat gaagcgcctt
     1561 cacaactacc cgctgggcag cctgccttat ggtctgcaaa aacgcgccga tgtcgcccgc
     1621 gcgctcgccc tacaaccgaa attgctgctg ctggacgagc cggtggccgg tatgggaggc
     1681 gaggaaagcg cggatatcgc ggcagccatc ctggatatca aagaacagct cggcatcacc
     1741 caggtgctgg tcgagcatga tatggcgatc gtgatgggca ttgccgaccg cgtcctggtc
     1801 ctcgacttcg ggcgcatcat tgccctggac acgcccgccg gggtgcaggc caatcccaac
     1861 gtgatcaaag cttatctggg cgaagaattt gaggggaaat agtatgtcct attttacaca
     1921 actcgccttc gccggtgtcg cgctgggctg catctacgcg ctgatcgggc tgggcttctc
     1981 gattattttt aaggccagcg aggtcatcaa ttttgcccag ggcgagctgc tgctggtcgg
     2041 ggcatatgtc gtctcatcgg gcatgttcga gtggcacctg ccgtttctgc tggcgctgct
     2101 gctcggcatc gtcgtcaccg tgctgatcgg cgtcctcttc gagcgcttcg tcctgcgccg
     2161 catgattggc cgcccggtct tctcgatcct gatgattacc atcgggctgg acatcgtcct
     2221 gcgcacggtg gtagaggtgc gctggggatc aaatcccatt cccgccgcct cgcccttcaa
     2281 tatcacaagc ggcattgatc gcttcggcat ccatttcggt gcgaacgata tctggaccat
     2341 tattgtcact atcatccttt gcgccgcgct ctttgttttc ttccgctaca cgaaatacgg
     2401 cctttcgatg cgagccac
//
LOCUS       DASZAK010000605         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_86655, whole genome shotgun sequence.
ACCESSION   DASZAK010000605 DASZAK010000000
VERSION     DASZAK010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86655"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            715..957
                     /locus_tag="VGT44_10630"
     CDS             715..957
                     /locus_tag="VGT44_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581294.1"
                     /translation="MDIKPGDPELNEHIMYAKCVKGHVLKDAEDWILCENLPDPYAMC
                     WQEGGDTIFSLAYKKRASAAENEAREASAAAQKTAS"
     gene            964..1980
                     /locus_tag="VGT44_10635"
     CDS             964..1980
                     /locus_tag="VGT44_10635"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV2581295.1"
                     /translation="MAKQFLITPPLPQPALLERVHSIILEWYARHQRDLPWRSTSDPY
                     AVLISEIMLQQTQVERVLPKYRQFLAAYPTLADLAAAPTSQVISNWVPLGYNSRAVRL
                     QAIARQVLAEHNGRIPDTVDALLRLKGIGRYTAGAIACFAYRQQVATVDTNIYRVLHR
                     IFFGLEQPEPAIKTEQMFDIAEQLMPPGEAYNWNQALMDLGATICTSGNPQCLRCPLQ
                     DICAAQVEMGRYSLFPGGTALGSLQQVRRKVAEKKAGYQAHPERPSQQSLPFTSSNRY
                     FRGRIVDYLRSLPPGQRIKPPELGTIIKPDFSHADLPWLQKLIVALQKDGLVDASEDG
                     VCLP"
BASE COUNT          478 a          559 c          532 g          448 t
ORIGIN      
        1 ctcttcttcc tggcgatggc ggccagttat ggggtaatct tgcagccgct cggctggctt
       61 tacctgctgg ttgcctttaa agtggggaca tggtggttcc ggcgttcggt cagccagaga
      121 gcttaagccc tagacaacgg aagataaaaa aatagggcag ctgagccgcc ctatttttgt
      181 ttgctgtgca tatcaatgct tacgcacgat ggctgcaacg ctggcacacc catgcgccat
      241 caacgatgga ccgtctgaaa ggcatctctt tattgcaatg cgagcaatag agaatcctgt
      301 cattcgacca gtgggtcaac cagtgccatg acctggcaat cataaaaggc tcctttgaag
      361 cttatcagtt ttcttatagt ttccagccca ccccaacaac tgcgaccttc agttagtcag
      421 aagtcagcga acttatacaa atattatagc acatctattc aattaatgca aattcaatga
      481 tgctatttaa cgcacaatca cataattgtg ataatggtat gttagcaaaa ttattaaaaa
      541 agtaacaagt ataccaccat ttgctccaat gtgccatttg ggttataata cgggcaggaa
      601 gccagaaatg ggcgcgaagt tatgcttccg cactcatcca gggttctaaa cttggagtaa
      661 ggaaggtaat agtctgtggc atggtgtaga ttttttgtac cgaagattgc ctcgttggac
      721 atcaagcccg gtgatccgga attaaacgag catatcatgt atgccaaatg cgttaaaggt
      781 cacgtgttga aggatgccga agactggatt ctctgcgaaa atctgcctga tccctacgcg
      841 atgtgctggc aggaaggcgg agatactatt ttttcgctag cgtataagaa acgagcatct
      901 gccgctgaaa atgaagcgcg cgaggcaagt gcagcagcgc agaagacggc ctcctgaggt
      961 tgcatggcaa agcaatttct tatcactcct cctttaccac agcctgcgct gctggagcgc
     1021 gtccatagca tcatcctgga gtggtacgcg cggcatcaac gcgatctccc ctggcgttcg
     1081 accagcgatc catacgccgt tctgatctcc gagattatgc tgcaacagac gcaggtcgag
     1141 cgcgtcctgc ccaaataccg gcagtttctg gccgcctatc cgacgctggc cgacctcgcg
     1201 gcagcgccga cctcgcaggt tatctctaat tgggtgccgc tcggctacaa ctcgcgcgcc
     1261 gtgcgcctgc aagccatcgc acggcaggtt ctcgccgagc ataacggtcg catccccgat
     1321 actgtggatg cgcttttgcg tttgaagggc attggccgct acacggctgg ggccatcgcc
     1381 tgtttcgcct atcgccagca ggtcgcgacc gtcgatacca acatttatcg cgtgctgcac
     1441 cgtatctttt tcggcctcga acagccggaa cccgccatca agaccgagca gatgtttgac
     1501 atcgcggaac aactcatgcc gccaggcgag gcgtacaact ggaaccaggc gctgatggac
     1561 ctgggagcga cgatctgcac gagtggcaat ccgcaatgcc tgcgctgtcc cttacaagat
     1621 atctgcgccg cgcaagtgga gatgggtcgt tacagcctct tccccggcgg tacagcgctc
     1681 ggctcgctgc agcaggtacg ccgcaaggtc gccgaaaaga aggcaggcta ccaggcgcat
     1741 ccagagcggc catcgcagca gtcgttgccc ttcaccagca gcaaccgcta ttttcgcggg
     1801 cgcatcgtcg attacctgcg ctcacttcca cccggccagc gcatcaagcc gccagagctt
     1861 ggcaccatca tcaaacccga tttcagccat gccgatctgc cctggctgca aaagctgata
     1921 gtggcgctac aaaaagacgg cctcgtggat gcaagcgagg atggcgtttg tctgccgtga
     1981 agcgagctga ggcaaagtcg agcaacaggc gagcgag
//
LOCUS       DASZAK010000606         2969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_406742, whole genome shotgun sequence.
ACCESSION   DASZAK010000606 DASZAK010000000
VERSION     DASZAK010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406742"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2877
                     /gene="carB"
                     /locus_tag="VGT44_10640"
     CDS             <1..2877
                     /gene="carB"
                     /locus_tag="VGT44_10640"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HEV2581296.1"
                     /translation="EKYNVRILGTPISAIMLTEDRQAFARHLRAIDIPTPQSRSAETL
                     EDALRIGEEIGFPVMVRAGFALGGQGSGIAHDRDELEHIVTGALAFAPQVLIEQYLHH
                     FKEVEYEVVRDRYDNCITVCNMENMDPLGIHTGESIVIAPSQTLTNGEYHLLRAASLA
                     IVRSLDIVGECNVQFALDPRTSAFYVIEVNARLSRSSALASKATGYPLAYVAARLALG
                     YGLTELTNRVTGVTRACFEPSLDYVVVKIPRWDLEKFKGAEETIGTGMKSVGEVMGIG
                     RTFEEAYQKAIRMLDLDFEGVTSEKTFGGNDDTAEIIRRYLHTPTPKRMFAIALAMQH
                     GFSVEEIGEITGIDAWFLDRIRFIVDTELSLRGDRQLSPSRLLALKQVGISDKRIGEL
                     VGKTGLEVRALRQSFGIAPSILQIDTLAAEFPSDTNYLYMTYNAQHSDVSPLGDDGVV
                     VLGSGPYRIGSSVEFDWTSVSTVEALKKHHKRAVIINCNPETVSTDYDTSDRLFFEEL
                     TFERIADICEFESPHGLIVSVGGQTPNNRAKALQRYGFRLLGTDAGYIDQAEDRNKFS
                     KLLDTLHIRQPEWNSLTDQAELRAFAERVGYPVLVRPSYVLSGNAMRVCYTPRELEQY
                     TREAAAVSPEYPVTVSRFFEKVKEIEFDAVAQRGQVKAFVISEHIENAGVHSGDATIV
                     LPTQTLNAETLAKIEAAGRAIAQALHITGPVNIQFLAQENQVYVIEVNLRASRTFPFI
                     SKVTGVNFIDLFVDALFYDDIPAVHIPAPSFVAVKAPQFSFSRLTGADPILRVEMSST
                     GEVACFGEDLEEAYLKAMLATGVHIPERGIFISLGGDEKKQKFLESARLLGASGIPLY
                     ATEKTCAFLRANGIEATMLYKIHEEKYPNVLTYFREHRIDLAINIVDGFVNKELDDDY
                     AMRRYAVDHNIPLFTKIKQARLFTRAIGIKDPAMLPVKAWNEYV"
BASE COUNT          600 a          964 c          832 g          573 t
ORIGIN      
        1 gaaaaataca atgtgcgtat cctgggaact cccatatccg cgatcatgct caccgaggac
       61 aggcaggcct tcgcgcggca cctgcgcgcc atcgacatac cgacgccgca gagccgcagc
      121 gcggagacgc tcgaagacgc cctgcgcatc ggcgaggaga tcggctttcc ggtgatggtg
      181 cgcgccggtt tcgccctggg cggccaggga tcgggtatcg cgcacgaccg cgacgaactt
      241 gagcatattg tgacgggcgc gctggccttc gccccgcagg tgttgatcga gcaatacctg
      301 caccacttca aagaggtcga atacgaggtg gtgcgcgatc gttacgataa ctgcatcacc
      361 gtctgcaaca tggaaaatat ggacccactc ggcatccata ccggcgaatc catcgtgatc
      421 gcgcccagcc agacgctgac caacggcgag tatcacctgc tgcgcgccgc ttccctcgcc
      481 atcgtgcgca gcctggatat cgtcggcgag tgcaatgtcc agttcgcgct cgacccgcgc
      541 acatcggctt tctacgtgat cgaagtcaat gcccgcctct cgcgcagttc cgccctggct
      601 agtaaagcca ccggctatcc cctggcctac gttgccgcca ggctggccct cggctacggc
      661 ctgaccgagc tgactaaccg cgtcacgggc gtcacgcgcg cctgcttcga gccgagcctc
      721 gactacgtcg tggtcaagat acctcgctgg gacctggaga aattcaaggg cgcggaggag
      781 accatcggca ccggtatgaa atcggtgggc gaggtgatgg gtattggccg caccttcgag
      841 gaagcctatc aaaaggccat ccgcatgtta gacctggact tcgagggcgt cacctccgag
      901 aaaactttcg gaggcaacga cgacaccgct gagatcattc gcaggtacct gcacacgccc
      961 acgccgaaac gcatgtttgc catcgccctt gccatgcagc acggcttcag cgtcgaagaa
     1021 attggcgaga tcaccggcat cgatgcctgg ttcctggacc gcatccgctt catcgtcgat
     1081 accgagctga gcttgcgcgg cgaccgccag ttgtcgccct cgcgcctgct ggccttgaag
     1141 caggtgggca tttcggataa acgcatcggc gagctggtcg gcaagacggg cctggaggtt
     1201 cgcgcgctgc gtcaatcgtt cggtatcgct ccctcgatcc tccagatcga cacgctggcg
     1261 gctgagttcc cctcggacac caactatctc tacatgacct acaacgcgca gcattccgat
     1321 gtctccccgc ttggggacga tggcgtggtc gtgctgggtt ccggccccta tcgtatcggc
     1381 tcctcggtcg agttcgactg gacgagcgtc agcacagtgg aggcgctcaa gaagcaccac
     1441 aagcgcgccg tcatcatcaa ctgcaaccct gagaccgtct cgaccgatta tgatacctcc
     1501 gaccggctct tcttcgagga gttgaccttc gagcgcattg ccgacatctg cgagttcgag
     1561 tcgccgcacg gcctgatcgt ctccgttggc ggccagacgc ccaataatcg cgccaaagcg
     1621 ctgcagcgct acggcttccg gctgctcggc accgatgccg ggtatatcga ccaggccgag
     1681 gatcgcaata agttctcgaa gctgctcgat acgctgcaca ttcgccagcc ggaatggaac
     1741 agcctcaccg accaggcgga actgcgcgcc ttcgccgagc gcgtcggcta ccccgtgctg
     1801 gtgcgtccat cctacgtgtt gtcgggcaat gccatgcgcg tctgctacac gccgcgagaa
     1861 ttggaacagt atacccgcga agcggcggcg gtttcgcctg aatatcccgt cactgtctct
     1921 cgcttcttcg agaaggtcaa ggagatcgag ttcgacgcgg tggcgcagcg cgggcaggtg
     1981 aaagccttcg tcatctctga acatatcgag aatgccggtg tgcattcggg cgatgccaca
     2041 attgtgctgc ccacgcagac gctcaacgcc gagacgcttg caaaaatcga agcggctggc
     2101 cgagccattg cccaggcgct gcatatcacc ggcccggtca atatccagtt cctggcgcag
     2161 gaaaaccagg tctatgtgat cgaggtcaat ctgcgcgcct cgcgcacctt ccctttcatc
     2221 tcgaaagtga ccggcgtcaa cttcatcgac ctgttcgtgg atgcgctgtt ctacgacgac
     2281 attcccgccg tccacatacc cgcgccctct ttcgtcgccg tcaaagcgcc ccagttctcc
     2341 ttctcgcgac tgaccggcgc cgaccccatc ctgcgcgtgg agatgtcctc caccggggaa
     2401 gtcgcctgct tcggcgaaga cctggaggaa gcctatctca aagcgatgct ggcgaccggc
     2461 gtccacattc ccgaacgagg tatcttcatc agcctgggcg gtgatgaaaa gaagcagaaa
     2521 tttctggaga gcgcgcgcct gctcggcgcg tccggcattc ccctctacgc taccgagaag
     2581 acttgcgcct tcttgcgcgc taatggcatc gaggccacca tgctctacaa gattcacgag
     2641 gaaaagtacc ccaacgtgct gacctatttc cgcgagcatc gcatcgatct cgccatcaat
     2701 atcgtggatg gcttcgtcaa caaagaattg gacgacgatt acgccatgcg ccgctacgcc
     2761 gttgaccaca acattcccct cttcaccaaa atcaaacagg cccgcctctt caccagggct
     2821 atcggcataa aagacccggc catgctgccg gtgaaagcgt ggaatgagta tgtataattc
     2881 tcacatagat ccaatgccag ggcgaccgca agggtcgccc ctaccctata cgaatgccca
     2941 tttccccatt tccaggtata gggtagggg
//
LOCUS       DASZAK010000607         5560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_121266, whole genome shotgun sequence.
ACCESSION   DASZAK010000607 DASZAK010000000
VERSION     DASZAK010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5560
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121266"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VGT44_10645"
     CDS             <1..411
                     /locus_tag="VGT44_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2581297.1"
                     /translation="DQGADDYLTKPFGVGELLARVKAVLRRNHWADQPVSAGTQRFGD
                     LEIDFEQRRVWKEGALVKLSPTEFELLQELALHPGKIFTHEALLRRVWGMEYHSESEY
                     LRVYIGRLRRKLENDPARPVYLLTEPGVGYYMVK"
     gene            complement(421..1722)
                     /locus_tag="VGT44_10650"
     CDS             complement(421..1722)
                     /locus_tag="VGT44_10650"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2581298.1"
                     /translation="MLATLRNRNFALLWSGGLISLMGDWVLNVGLPIYVFLLTRSVLI
                     LSITVLLNALPNILLGSVAGVFVDRWDRKRTVIITNLLLGLSLLPLLLVRAADRVWIV
                     YIVAFVGASIEQFFMPAQSALLPTLVAEAHLVQANSLNSLSSNFARLVGPAIGGIVAA
                     IFGLTGIVLADMASFLIAGLLITFVKIAATSNVQAETSPPDNEIITKASFWRELVDGL
                     RVIRSERTLAVILAMYSILMVGEGVFAVLYPVFVNRILHGGAAQIGQLMSAQAIGGLL
                     GGLLIGWLGRRVMSRWTIGLFGMAFGLTDLAIFNTPAFFPFFWLTAGLFVTAGLVGIA
                     PMTGAQSLLQAKSPEAYRGRVFGVLGMLMGLCALIGTIAAGFLTDHLGLITVLNVQAA
                     GYVCAGLLALSLLPRERKTTSGRASKFDHVTQQSIAEESAG"
     gene            1986..3113
                     /locus_tag="VGT44_10655"
     CDS             1986..3113
                     /locus_tag="VGT44_10655"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF005758.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HEV2581299.1"
                     /translation="MHDATRVIRAGLHEAVQGAPFLPGPTFASAYHFAGEVETSPYTY
                     GRYHNPTWTAFEQALSELEGGHALVFASGMAAVTAVLGVALHTSGGTEKSKVLVMPSD
                     SYYTARMLADGFFASTGIEVRKAPTAGNALGAFLDGARLLWLETPANPGMDVCDLAEL
                     IAEAHQRGVLVAVDNTTATVLGQRPLELGADFSVASDTKSLTGHSDIMLGHVAVRDPV
                     WTERLLAFRSQQGAIPGPMEVWLAHRSLATLDMRLERQCRNAQAIAQMLAERPEVRLV
                     RYPGLPQDPAHAIASRQMKHYGPVMSFVLADKALADRFLAACRLVLTATSFGGICTTA
                     ERRARWGGDAIPEGFIRLSAGCEDTQDLLEDISQALDMCIF"
     gene            complement(3116..4405)
                     /locus_tag="VGT44_10660"
     CDS             complement(3116..4405)
                     /locus_tag="VGT44_10660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2581300.1"
                     /translation="MSSRLTSSPDFVIRSTETVDEIDTYFRLNAETFRPDEDPLVVAA
                     RRRRMVMRDPNFQQIQLRSAFYGNNYIGSCRIQERWLSLEASRLHVGCVGGVVTHPDY
                     RHQGVATALMQDAFEVAKEKRYDLLLLHGIADYYKQHGYIDVFEDMPRHTIERALIPA
                     QPVEDRIVRLAEAQDAPALLALYQEHYGNSMCTFAPTRTLERQVHYLENWFQENIPLL
                     AVNTQGNAEGYLLLSRRRGQLYAYEAAANTWPATLALLQHHHHLLEAEAESQATLFWP
                     LPPGGLTYYLLADQLPIQSEMDSYPDGGWMALLVSFSTFVQALLETWQARWRQHRLAW
                     TGVLALVVDGQICLLDITPEHISLVDSSSTETRQVTLSAQVFTQLALGFRPVSWAIIQ
                     EAQSIPVDLVPVLDVLFPCKQTGIARSDDWIAGSDYF"
     gene            4514..>5560
                     /locus_tag="VGT44_10665"
     CDS             4514..>5560
                     /locus_tag="VGT44_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV2581301.1"
                     /translation="MQDIRIDRWAHTLVNYSLSLEAGETVAIRSTPLAAPLIEAVYRE
                     ALRVGAHPMPIIELEGLEELLLREGSDEQLAVVHPYITATAEHVDAQLTIGAKSNTRA
                     MTNIEPSRLAKRRQASYGFRQTQRNREMAGQYRWASTLYPTPGYAQDAEMSLSEFEEF
                     VFDVCFLNDADPIARWKEVSAKQQRLVDWLVGRRRVHILGKQTDLTLAIDGRIFINSD
                     GKRNFPSGEFFTGPVESSANGVIQFDIPSSFDGHAFEGVRIVFREGKVVEASARQGQA
                     FLDEMLALDEGARYLGEFAFGNNPRVTRGIKNILFDEKMGGTVHLALGNSYPETGGLN
                     HSALHWDMVCDLRVD"
BASE COUNT         1255 a         1622 c         1597 g         1086 t
ORIGIN      
        1 gaccagggcg ctgacgacta cctgaccaag ccgttcggcg tcggcgagct gctggcgcgc
       61 gtcaaggccg tgctgcgccg caaccactgg gcggaccagc cggtttcagc cggtacgcag
      121 cggttcggcg acctggaaat cgacttcgag cagcggcgcg tctggaaaga gggcgcactg
      181 gtcaaacttt cgcccacaga atttgaatta ctccaggaac ttgccctgca tcccggcaag
      241 atttttaccc acgaagcgct gctcaggcgc gtctggggca tggaatacca tagcgaatcg
      301 gagtacctgc gcgtctacat cggtcgcctg cgccgcaagc tcgaaaacga tcccgcccgg
      361 cctgtgtacc tgttgaccga accgggagta ggctactaca tggtcaaatg aggcatggca
      421 ttacccggca gactcctctg ctattgactg ctgggtgaca tggtcaaact tcgatgctcg
      481 ccctgacgtt gttttcctct cgcgcggcag cagcgaaagc gccagcagcc cggcgcacac
      541 ataacccgcc gcctggacgt taaggacggt gatcagtccc aggtgatcgg tgaggaaacc
      601 ggcggcaatc gtgccgatca gggcgcacag ccccatcaac atacccagca cgccgaagac
      661 gcgcccgcga taggcttcgg gcgacttcgc ttgcaacagc gattgcgccc ccgtcatagg
      721 agctataccg accagtccgg cagtcacaaa gaggccagcg gtgagccaga agaatgggaa
      781 gaaggcaggg gtattgaaga tggcgagatc ggtcagccca aatgccatgc cgaatagccc
      841 gatggtccag cgcgacatga cccgcctgcc caaccatccg atcagcaacc cgccgagcaa
      901 gccgcctatg gcctgcgcgc tcatcaactg gccgatctgc gccgctccac catgcaggat
      961 gcgattgacg aagaccggat aaagcacggc aaacacaccc tcgcctacca tcaagattga
     1021 gtacatcgcc agtatcacgg caagtgtgcg ttcagaacgg atcacgcgca atccatccac
     1081 cagctcgcgc cagaaactgg ctttggtgat tatttcgtta tcaggagggg atgtctcggc
     1141 ctgcacattg gatgtagctg ctatcttgac gaaggtaatg agcagacccg cgataagaaa
     1201 tgacgccata tccgccagga cgatgccggt aagcccaaaa attgccgcga cgataccacc
     1261 gattgccggc ccgacgagcc gcgcaaaatt actgctcaac gagttgagcg aattggcctg
     1321 caccaggtgt gcttcagcga ccagggtcgg caacagcgcg ctctgcgcag gcataaagaa
     1381 ttgttcgatg ctggccccca cgaatgccac aatatacacg atccagacgc gatcagccgc
     1441 gcgtacgagc aagagcggca agagcgacaa ccccaataag aggttcgtga tgattacagt
     1501 gcgttttcga tcccagcgat ccacaaaaac gccagcgacc gaaccgagga ggatgtttgg
     1561 gagggcattt aacagcaccg taatactcag aataagcacg gagcgagtga gcaggaagac
     1621 ataaatcggc aagccgacgt tgaggaccca atcgcccatc agtgagatca atccccccga
     1681 ccagagcagc gcgaaattgc ggttgcgcaa tgtagctagc atggtgccat ccttccggtg
     1741 aatttaagac aagaaaagtc attgacttgg taatattcac ttattcatta acacaatcat
     1801 acatttacca gatatatttg tcaaatttaa tggtaatatt catcatttca ttgcgattta
     1861 ttgaccacat tttttctttt tgctggtcgt cttctctatc gctaattggc tcttttacga
     1921 tagaccctga cagggtacga tatcttcaaa ctcagcacac cgaccaacga gaggagcaat
     1981 ctaccatgca tgacgcaaca agagttattc gcgccgggct gcatgaagcc gtgcaaggcg
     2041 cgcccttttt gccggggcca accttcgcca gcgcctatca tttcgcggga gaggtcgaaa
     2101 cgtcgcccta tacctacgga cggtaccaca atccgacctg gacggctttc gagcaggcgt
     2161 tgagcgagct tgagggcggt cacgcgctgg tcttcgcctc cggtatggcg gcggtcacgg
     2221 cggttctggg agtggcactg catacgagcg gagggactga gaagagcaag gttctggtga
     2281 tgccttcgga tagttactat acggcacgta tgctggccga tggctttttc gcttcgacag
     2341 gcatcgaggt gcgcaaggcg ccaaccgcgg ggaacgcact gggcgcgttc ctcgacgggg
     2401 caagactgct ctggctagaa acgcccgcta atcccggtat ggacgtgtgc gacctggccg
     2461 aactgatcgc ggaggcacac cagcgcgggg tgctcgttgc tgtagacaac acaaccgcca
     2521 ccgtgcttgg gcagcgaccc ctggaactcg gcgcggattt ttcggttgcc agcgatacaa
     2581 agtccctgac gggtcacagc gatatcatgc ttggccatgt cgcggtgcgc gatccggtgt
     2641 ggacagaacg cctgctcgcc tttcgcagcc agcagggcgc aatacccggc ccgatggagg
     2701 tctggctggc tcatcgctcg ctcgccacgc tggatatgcg cctggaacgg caatgtcgca
     2761 atgcccaggc gattgcccag atgctcgcag aacggccaga ggtacgcctg gtacgctatc
     2821 ccggattgcc gcaggacccc gcgcacgcca tcgcctcccg ccagatgaaa cactacggcc
     2881 cggtgatgag cttcgtgctg gcggacaaag cgctggcgga ccgtttcctg gcggcctgcc
     2941 gactggttct gaccgccacc agcttcggcg gcatctgcac gaccgccgaa cgccgcgcgc
     3001 gctggggagg cgacgccatt cccgaaggct ttatccgcct cagcgccggc tgcgaggaca
     3061 cacaggactt gctcgaggat atcagccagg cgctggatat gtgtattttc tagaactaaa
     3121 aatagtccga acccgcaatc cagtcgtccg atctagcaat cccggtctgt ttgcatggaa
     3181 aaagcacatc aaggacagga acaagatcaa ctggaataga ctgcgcttcc tggatgattg
     3241 cccaggagac gggacggaag ccaagcgcaa gctgtgtgaa gacctgcgcg ctgagcgtta
     3301 cctgccgcgt ttcagtagat gagctatcca ccaggcttat atgttcagga gttatatcaa
     3361 gcaagcatat ttgcccatca acgaccagtg cgagcactcc tgtccacgcg agacggtgtt
     3421 gtcgccagcg agcttgccag gtttccagga gcgcttgaac gaaggtcgag aatgagacaa
     3481 gcagagccat ccaaccaccg tcagggtagg agtccatctc gctctggata ggcaactgat
     3541 cggccagcag ataatacgtt agtccgccag gaggaagagg ccagaacagc gttgcctggg
     3601 attccgcttc tgcttcgagc aaatgatggt gatgttgcag caatgccagc gtcgcgggcc
     3661 atgtattggc cgccgcctcg taagcgtaaa gctgccccct ccttctcgat aggagcaggt
     3721 atccctcggc attgccctgt gtgttgactg ccagcaatgg aatattttcc tggaaccagt
     3781 tctccaggta atgaacctgg cgttccagcg tgcgggttgg cgcgaacgta cacattgaat
     3841 ttccatagtg ttcctggtat agagccaaca gggcgggtgc atcctgcgct tcagctaaac
     3901 gcacaatgcg atcctctacc ggttgagcgg gaatgagcgc tcgctcgatg gtatgacgcg
     3961 gcatgtcctc gaacacatcg atgtagccat gttgtttgta gtaatcggcg atgccgtgca
     4021 agagcagcag atcgtatcgc ttttcctttg caacctcgaa agcgtcttgc atgagcgccg
     4081 ttgctactcc ctggtggcga tagtcagggt gcgtcaccac gccgccaacg cagcctacgt
     4141 gcagcctgga tgcttccagg gataaccagc gttcctgaat gcggcagctt ccaatgtagt
     4201 tgtttccgta aaaggcgctc cgcaactgga tttgttgaaa attcgggtcg cgcataacca
     4261 tcctgcgccg ccgtgccgcc actaccagcg gatcttcatc ggggcgaaag gtctcggcgt
     4321 tcaagcgaaa ataggtatcg atttcatcga cagtttcggt tgaccgaata acgaaatcgg
     4381 gcgacgatgt cagtctgctg ctcattctgt cccttcacgg ataggcttga cgctatcgct
     4441 atgataataa catcacgaag aaaaaccaac tcaacccacg acattatact atctcacatg
     4501 gaaaggctct catatgcaag acatccgtat cgatcgctgg gcgcacacgc tcgttaacta
     4561 cagcctctcc ctcgaagcgg gcgagacggt cgccattcgc tctacgcccc tggcagcgcc
     4621 gctcatcgag gcggtgtatc gcgaagctct gcgcgtgggg gcgcacccaa tgccgatcat
     4681 tgaactggaa gggctggaag aactgctgct gcgcgaggga agtgatgagc agcttgcggt
     4741 ggtgcatccc tacatcacgg caacggctga acacgtggat gcgcagttga ccatcggcgc
     4801 gaaaagcaac accagggcga tgaccaacat cgaaccttca cgactggcga agcggcggca
     4861 ggcatcttat ggctttcgcc agacgcagcg caatcgcgaa atggcagggc aataccgctg
     4921 ggcatccacg ctctatccca cgcctggcta tgcccaggac gcggagatgt cgctgagcga
     4981 attcgaggaa tttgtcttcg atgtgtgttt cctcaacgat gccgacccca ttgcgcgctg
     5041 gaaagaggtt tccgctaagc agcagcgcct ggtggactgg ctggttgggc gcaggcgcgt
     5101 ccatattctg ggcaagcaaa ccgacctgac gctcgctatc gatggccgga tcttcatcaa
     5161 tagcgacggc aaacgcaact tccccagcgg cgagtttttc accgggccgg tcgagagcag
     5221 cgccaacggc gtgatccagt tcgatattcc ctcgtcgttc gatggccatg ccttcgaggg
     5281 tgtgcggatc gtcttccgcg agggcaaagt cgtggaagcc agcgcgcgcc aggggcaggc
     5341 gttcctggac gagatgctgg cgctggacga gggcgcgcgc tacctgggcg aattcgcctt
     5401 cggcaacaac ccgcgggtga cgcgcggcat caagaacatc ctcttcgacg aaaagatggg
     5461 cggcactgtc cacctggcgc tcggcaacag ctacccggaa accggcggac tcaaccattc
     5521 cgcgctgcac tgggacatgg tctgcgatct gcgcgtggac
//
LOCUS       DASZAK010000608         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_406786, whole genome shotgun sequence.
ACCESSION   DASZAK010000608 DASZAK010000000
VERSION     DASZAK010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406786"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VGT44_10670"
     CDS             complement(<1..817)
                     /locus_tag="VGT44_10670"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEV2581302.1"
                     /translation="MAIKLTAASKTDVGKQREQNEDSPFTHISEDGDRGLFIVADGMG
                     GYQAGEVASKLAVQKISESLRSFLVPVYEQPTIKLTPFSEQETIKLDPANEPAAKDHK
                     TRKLPETPVTKNVEEQLKAAIRQANKAILSYGEEQSSARGLGCTVTTALVQDDHAYIG
                     NIGDSRTYLFRGGELTPLTKDHSLVARLVEAHQIDPEDVYSHPQRNLIYRSLGAGHKS
                     VDPDVFHVTLRSGDSLLLCSDGLWEMVRHQELLKVLKENRDAQKICDILIDLAN"
     gene            complement(1085..1453)
                     /locus_tag="VGT44_10675"
     CDS             complement(1085..1453)
                     /locus_tag="VGT44_10675"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2581303.1"
                     /translation="MAEKGDGAWYVISVAAFKAEVHEQTLRHYERLGLISPARKGNSP
                     HSPRLYSDRDIERVVHIRGLMRDLGVNLAGVEAILHMKDRMERMQQELELEMERMQEQ
                     HTAETRRLKEIIERLQRGSP"
     gene            complement(1466..>2218)
                     /locus_tag="VGT44_10680"
     CDS             complement(1466..>2218)
                     /locus_tag="VGT44_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEV2581304.1"
                     /translation="RTYSYRGSPMDFDSATGFSDFFEALFGRSSAAGSRTNRNDFRRR
                     VGDNIEQPVEVTLQEAYLGGTRTFNIQSTEVCPLCRGTGEVAGKVCTNCSGQGMLARN
                     KRIQVKIPSGVDNGSRIRVAGEGQPGIGGGPRGDLFLVISVKLDSIYERKGDDLYADI
                     DVDLVTAMLGGEVPVPTPDGRRLILTIPAETQNGRMFRLANKGMPRLRGDGNGNLFAR
                     VKVVLPMGLTGEERKLFEQLARSRGAEVHSTG"
BASE COUNT          472 a          653 c          543 g          550 t
ORIGIN      
        1 cattagccag gtcgatcaag atatcacaga tcttttgggc atccctgttt tctttgagca
       61 ctttcagcaa ttcctggtgc cgcaccatct cccacaaacc atcggagcag agcaacaggc
      121 tatcgccgga acgtagagtt acatgaaaca catccgggtc aacgcttttg tgccccgctc
      181 ccagcgagcg atagattaaa ttgcgctgcg gatgcgagta aacatcttcg ggatcaatct
      241 ggtgagcttc caccagcctg gctaccagcg agtgatcttt cgtcagaggt gtaagctcac
      301 caccgcgaaa gagatatgtg cggctgtcac caatattccc aatgtaggca tgatcatctt
      361 gaactagtgc cgtagtcacg gtacagccca gcccgcgcgc agaagattgc tcttcgccat
      421 aactgagaat agccttgttg gcctgccgga tggcagcttt gagctgctcc tccacgttct
      481 ttgtcaccgg tgtctccggt agtttgcgcg tcttatggtc ctttgcggct ggttcattgg
      541 ccgggtccaa tttgatcgtc tcttgctcag aaaacggcgt cagcttgatg gtcggctgct
      601 cgtatacggg caccaggaag cttctgagcg actcgctgat cttttgtaca gcaagtttac
      661 tggcaacttc acccgcctgg tagccaccca tgccatcagc gacaataaag agccctcgat
      721 ccccatcctc tgagatatgt gtaaatggac tatcttcgtt ttgttcgcgt tgcttcccca
      781 cgtcagtttt agaggcggca gtaagtttta tcgccatcgg tatttccctc gcagtaaaga
      841 acttttatcg tcttttaggt caatataaca tatagcagta ttcatgacaa tcggtatttt
      901 ctggcttcag ggcttttctt cttcattcgc tgctccaggc aactggcagg agttaagaat
      961 ctgggatacc cccaggccca acttcatcca tatttattgc tgattcttca tttaatataa
     1021 cttacaacat atacgaggct tgggtgaaaa ggttgtgact gcgcgaggac gagacacccc
     1081 gctgctacgg ggagccgcgc tgcaaacgct cgattatctc cttgagccgg cgcgtttcgg
     1141 cggtgtgttg ctcctgcatg cgctccattt ctagctccag ctcctgctgc atgcgctcca
     1201 tgcggtcttt catgtgcagg atagcctcaa cgcctgccag gttaacaccc aggtcccgca
     1261 tcaagccacg gatatgcact acacgttcga tgtcgcgatc tgaatagaga cgcgggctat
     1321 gcgggctatt gcccttgcgc gcaggcgaga tcaatccaag tcgctcataa tggcgcagtg
     1381 tttgctcatg cacctcggct ttgaacgctg ccacacttat tacataccat gcgccatcgc
     1441 ctttctcggc caccttcgat cctccttatc ctgtcgagtg tacttcggct ccccggctgc
     1501 gcgccagttg ctcaaacaac ttgcgctcct caccggtcag gcccatgggc agcacgactt
     1561 tgacacgagc aaagaggtta ccattgccgt caccgcgcag gcgcggcatg cccttgtttg
     1621 ccaggcggaa catgcgcccg ttctgcgtct ccgctgggat ggtcagaatg agtctgcgcc
     1681 cgtccggggt gggtacaggc acttcgcctc ccagcatcgc cgtaaccagg tccacatcta
     1741 tatcagcgta cagatcatcc cccttgcgct cgtaaatcga gtcgagtttg acgctaatca
     1801 cgaggaagag atcgccgcgc ggtccgccgc caatgcccgg ctgtccctca cccgccacac
     1861 gaatcctgga accattatca actcccgacg gaatcttcac ctggatgcgt ttattccgcg
     1921 caagcatacc ctggccgcta caattcgtac aaactttgcc cgcgacttca cctgtgccac
     1981 ggcagagcgg gcatacttcg gttgattgaa tattgaacgt gcgcgtccct cccaggtatg
     2041 cttcctgtaa cgtcacctcg acaggctgtt caatattgtc gccgacgcgc ctgcgaaaat
     2101 cattgcgatt ggtgcgtgac cccgccgctg aggatcgacc gaaaagagct tcaaagaaat
     2161 cggagaatcc cgtagccgaa tcaaaatcca taggtgagcc acgataacta taggtgcg
//
LOCUS       DASZAK010000609         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_121442, whole genome shotgun sequence.
ACCESSION   DASZAK010000609 DASZAK010000000
VERSION     DASZAK010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121442"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(347..1702)
                     /locus_tag="VGT44_10685"
     CDS             complement(347..1702)
                     /locus_tag="VGT44_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012812390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEV2581305.1"
                     /translation="MTECTQNSFEFQGHFSRAVRAQFDGGAMTSDAGGLLLRETDRRM
                     NLLPRLAQCFLDGRNPARVEHPVGQMVAQRVYGLALGYEDLNDHEQLRQDPLLKLLAG
                     QPDLDQPLAGKSTLNRLELSTGSPDRYKKITFWKQAIDELLVEVFVEAHTSTPQEIVL
                     DIDTTDVALHGDQEGRFFHGYYDHYCYLPLYVFCGEHLLCARLRPANIDASAGSLSEI
                     RRIVEQIRKHWPQVRITLRGDSGFCRDELMSWCENNGVDFVFGFARNPRLRALVADAL
                     IQAQQQWEQTRKPARVFVEFSYETTSGSWSQARRVVAKAEHIEGKENPRFVVTSRSTQ
                     DWPAQKLYEELYCARGDMENRIKEQFVLFADRVSAETMRANQLRLYFSSMAYVLLSGL
                     RRIGLKATELATAQAATIRLRVLKIGALVRITVRKVWISLPRSYPWPDLFAQLHRALS
                     G"
BASE COUNT          379 a          560 c          604 g          393 t
ORIGIN      
        1 tccgccgctt cgtcgatgcg gattcagtgg cgtatctctc gctctccggc ctgcgtgaag
       61 ccgtcgcgga ttacaaccac gactactgct acgcctgtta caccggcacc tacccgaccg
      121 agctcatcaa catcgacgag ctgatggccg ccaaggaccg aagaggatag aagcaagctt
      181 agcccgcatt tctcaccagt gacgaggtag gtgtttggag cgaggagggg aggggcgctg
      241 gttgaaggag tgccgatatc gagccgctag gggttgtgtg gagacgcagt ggtgccacaa
      301 gaagtttttt gggaaccggt ttgtcaaaga gcgcgatcag acgagttcat ccactcagag
      361 ctcgatgcaa ctgagcgaac agatcgggcc aagggtagct gcgcggcagc gagatccata
      421 ctttgcgtac ggtgatgcgc accagggctc cgatttttag cacccgcaga cggatggtgg
      481 cggcctgagc ggtggccagt tcggtggctt tcagtccgat gcgccgcagc ccgctcagca
      541 gcacataggc catggaggaa aaatacagac gcagctgatt ggcgcgcatg gtctcggcgc
      601 tgacgcgatc ggcaaacaga acgaactgtt ccttgatccg gttttccatg tcgccgcggg
      661 cgcagtaaag ttcctcgtac agcttctggg cgggccagtc ctgggtcgat cgcgaggtca
      721 ccacgaagcg ggggttttcc ttgccctcga tatgttcggc cttggcgacc acgcgccgcg
      781 cctgggacca ggaaccggag gtggtttcat aggagaactc gacaaacacg cgtgccggtt
      841 tgcgggtctg ttcccattgc tgttgggctt ggattaaggc atcggccacc agggcgcgca
      901 agcgcgggtt gcgggcgaag ccgaaaacaa aatccacgcc gttgttttcg caccagctca
      961 tcaactcgtc gcggcagaag ccggagtcgc cgcgcagcgt gatgcgcacc tggggccagt
     1021 gtttccggat ctgttcgacg atgcggcgaa tctcggaaag acttccggcg gaagcgtcga
     1081 tgttggccgg gcgcaggcgc gcgcacagca gatgctcgcc gcagaacacg tacagcggca
     1141 gataacagta gtgatcgtaa tagccgtgaa agaagcggcc ttcctggtcc ccgtgcagcg
     1201 ccacgtcggt ggtgtcgatg tccaatacga tctcctgagg tgtgctggtg tgcgcttcca
     1261 caaatacctc caccagcagt tcgtcgatgg cctgcttcca gaaggtgatt ttcttgtatc
     1321 gatctggtga gcccgtgctc aactccagcc ggttgagcgt gctcttaccg gccagcggct
     1381 gatccagatc gggctggccg gccagcagct tcagcaacgg atcttggcgc agttgttcgt
     1441 ggtcgttgag atcttcgtag cccagcgcca gcccgtacac gcgttgcgcc accatctggc
     1501 ccacggggtg ctcgactcgc gccggattgc ggccatccag aaagcactgc gccagccgcg
     1561 gcagcaggtt catccgccgg tccgtctctc gcaacagcaa gccgcctgca tcggaggtca
     1621 tcgcgccacc gtcaaactgc gcccgcaccg cgcgcgaaaa atggccttgg aactcgaaac
     1681 tgttttgggt acactctgtc atagcgaaag ccgctttctc cttggcgcta actgctgtct
     1741 ggaaactcag ttatgccaga gtcggcggct ttttgctata gcgttcgtga gaaatgcggg
     1801 ctagcctgcg agatgacgcc ctcggcccga gtgcaggccc atcgaaccag ggcggctacc
     1861 taaaaattag gcggcgtatg caaactgcat gttattggca gttttggttt tccgatcgtt
     1921 taacgggagc acggct
//
LOCUS       DASZAK010000610         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_655459, whole genome shotgun sequence.
ACCESSION   DASZAK010000610 DASZAK010000000
VERSION     DASZAK010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655459"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VGT44_10690"
     CDS             <1..400
                     /locus_tag="VGT44_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2581306.1"
                     /translation="HLFDLFPDLRTAARLKAGNLSGGQRNMLAMARGLMLSPEVLLLD
                     EPTAGLSPLYTTVVWEKVRDIAQSGTTVVVVEQNVDRALANSDWVYVMVAGRNRVDGT
                     PEHMRSIDLPAIFLGKKGESTAEELPVTDA"
     gene            551..1804
                     /locus_tag="VGT44_10695"
     CDS             551..1804
                     /locus_tag="VGT44_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2581307.1"
                     /translation="MKSKLFTLMTIVILSTFALSACGGTNTPGTGGNTASPITVGELF
                     PMTGREPFVGQWFLHGAKVGIADINANGGCGGHQINAQLADTGGDPVDAVTALRQLQL
                     SNPAFILGPSSLEIEGVINQFDPQHLPDFVEGGTTQLDHMNNQYVYRTTPSDSTLAIG
                     MAYYAIQKNLTKAALFFESTSNAAGVVSPLVKAYTGHGGTLTAQLLVTPHQSSYRTEV
                     EKLFAGNPQAIFMQTDPQTASTFFSDVRQLGHMNVPFIATDAGADINLAKAMGLADAS
                     KWLTGMNGSPPAGPAWQYFKTQYQKVWNSPNPVTLSQNTYDSVVIACLAITAAGTTDP
                     TVWINDVTKVANPPGQVVTSYAEGVAALKAGKKINYDGASGKDDYNQFHNVAGGWDIV
                     QFDATGANLNTVASVSEDQIAAWNV"
     gene            1986..3026
                     /locus_tag="VGT44_10700"
     CDS             1986..3026
                     /locus_tag="VGT44_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2581308.1"
                     /translation="MKTRSRKPGLVACIILALILGACGSPGVGGEGGNSNLPISLRIM
                     VGGLDKQIYLPNMLAQRLGFFAQEGLDVTLIDEASGQSAEDEVMASHVEAGSGSYNHT
                     IELQASGKHMECVVQLIDAPGEAEVVSTKAANTIRSVKDLVGKDLGVTELGAGTQTLT
                     ILLLHNAGIAANQVDFVPVGAGDTFIAAMQQGVIDAGMTTEPTISQLVSGGVGRVLVD
                     LRTPATTQAALGGPYPFICVFMQNSYVNAHKAIVQRLVNAYVMTLKWIHTHTAQQITD
                     MMPADYYADGKAQYVQALQNQLQMYSPDGLMPAGGPQFVLQTENETNFAVKGQSIDLS
                     ATYTNEFVNNAP"
     gene            complement(3030..3734)
                     /locus_tag="VGT44_10705"
     CDS             complement(3030..3734)
                     /locus_tag="VGT44_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2581309.1"
                     /translation="MTTRTKRILLAEDELALRDFVSRNLRARGFDVLEASNGLEAMAT
                     WEQEHPHLLILDIMMPRMDGLEVCRRVREHSAVPIIVLTALDAESDKVTALALGADDY
                     LTKPFGVEELLARVRAVLRRTQWDIPQPSGDIKQFGELEIDLSGHIVRLRGAEVRLSP
                     TEFAVLKQLILHAGKVLTHRMLLQSIWGAEYGGEAEYLRVYVNRLRQKLEPDPANPRY
                     LLTEPGVGYRFAAPRP"
BASE COUNT          805 a         1082 c         1152 g          752 t
ORIGIN      
        1 tcacctgttt gatctctttc ctgacctgcg caccgcagcg cggctgaaag cagggaattt
       61 gagcggcggc cagcgcaata tgctggcgat ggcgcgcgga ctgatgctca gcccggaggt
      121 attactgctg gatgagccga ccgccggcct ctcccctctt tatacaaccg ttgtgtggga
      181 aaaggtgcgc gatatcgcgc aatcgggtac gacggtcgtg gtggtggaac aaaatgtgga
      241 tcgtgccctg gccaactctg attgggtcta tgtcatggta gctggtcgca atcgggtgga
      301 tggaacacct gaacatatgc gcagtatcga tctgcccgcc attttcctgg gcaagaaagg
      361 agaaagcacg gctgaagaat tgcccgtcac cgatgcctga cccggcaatt tgccgattga
      421 ggcataaagc ggcctcgcgc ctcgttcgag cgaaatggtg agcaacatcg gagatgtgga
      481 agcatcacac ccgcgacatg acgaacatgt cgccagtccc ataggtattc tcacgacgat
      541 gagggagaac atgaaaagta agctatttac cttgatgaca atcgtcatct tgagcacatt
      601 cgctttaagc gcgtgcggag ggaccaacac acctgggaca ggagggaata ccgcttcacc
      661 gatcacggtg ggcgaactct tcccgatgac ggggcgcgag ccattcgtcg gccagtggtt
      721 cctgcacggg gcgaaagtcg gtatcgccga catcaacgcg aatggcggat gcggtggaca
      781 ccagatcaat gcccaactgg ccgacaccgg cggtgacccg gttgatgccg taaccgccct
      841 gcggcagtta cagttgtcca acccggcctt cattctcggc ccatcatccc tggagatcga
      901 gggcgtcatc aaccagtttg atccacagca cctccccgac tttgttgagg gcggcacgac
      961 gcaactcgac cacatgaaca atcaatacgt ctaccgcacg accccttccg actcgacgct
     1021 ggctatcggg atggcctact acgcgatcca aaagaatctg acgaaggcgg cgctcttctt
     1081 cgagtccacc tccaatgcgg cgggcgttgt atccccactg gtcaaggcgt ataccggtca
     1141 tggcgggacg ttgactgccc aactactggt cacgccgcac cagagttcgt atcgaaccga
     1201 ggtagaaaag ctgtttgccg gcaatccgca ggcaatcttc atgcagaccg acccgcagac
     1261 tgccagcacc ttcttcagcg atgtgcggca gctggggcat atgaatgtcc cgttcatcgc
     1321 gaccgatgcc ggggctgaca tcaacctcgc gaaggcgatg ggtctcgcag atgcttccaa
     1381 atggctcacg ggcatgaacg gctcgccgcc ggccggcccc gcctggcagt acttcaagac
     1441 ccagtatcag aaggtgtgga acagccctaa cccggtgacg ctctctcaga acacctacga
     1501 ctcggtcgtc atcgcctgcc tggcgatcac tgccgccgga acaaccgatc caacggtgtg
     1561 gatcaacgat gtgaccaaag tggcgaaccc gcctggccag gtcgtgacga gctatgccga
     1621 gggagtagcc gctctgaagg ccgggaagaa gatcaactac gacggtgcat ccggcaagga
     1681 cgattacaac cagttccaca acgtggcagg tggctgggat atcgtgcagt ttgatgcgac
     1741 cggcgcaaat ctgaacacgg ttgcgtctgt ttccgaagat cagattgctg cctggaacgt
     1801 ctaggcgcga ttgatgttgt cagcaacgag cgcattcacc ccgctggttg ggtgaatgcg
     1861 ctcgttgcgt gtgaccgggc gtggcgtggc tgcattcatc attgcgcccc tactagacaa
     1921 tccaggacga ttcgggtata cctatagagg ccaactggtc atcggagtca aagaaggagc
     1981 gcgcgatgaa aacgcggagt cgcaagcctg gcctggttgc ctgcattatc ctggcactca
     2041 ttttgggcgc gtgtggcagc cccggcgtcg gcggtgaggg tgggaatagt aacctgccaa
     2101 tctcgctcag gatcatggtt ggcggtctgg acaagcagat ctatttgccg aatatgcttg
     2161 cccagcggct cggtttcttc gcgcaagagg ggctggatgt gaccctgatc gatgaagctt
     2221 ccgggcagtc tgctgaagat gaggtgatgg ccagccacgt cgaggcagga tcgggatcgt
     2281 acaatcatac catcgaactc caggcgtctg gcaagcatat ggagtgcgtg gtgcagctca
     2341 tcgacgcgcc cggtgaggcc gaagtcgtat cgacgaaggc ggcgaatacc atccggtcgg
     2401 tgaaagacct ggttggcaaa gacctgggcg tcaccgaact aggggcaggg acgcaaaccc
     2461 tgacgatcct gctgctgcac aacgcgggga tcgcagcgaa ccaggtggat ttcgtgccgg
     2521 tcggcgcggg tgataccttc atcgcggcta tgcagcaggg cgtcatcgac gcgggtatga
     2581 cgaccgagcc gacgatttca caactcgttt cgggcggcgt tggccgcgtg ctggttgacc
     2641 tgcgcactcc cgccactacg caagctgcgc tgggcgggcc atatcccttc atctgcgtct
     2701 tcatgcagaa ttcctatgtc aatgcgcaca aagcgattgt ccagcggctg gtcaatgcct
     2761 atgtcatgac cttgaagtgg attcatacgc atactgccca gcagatcacg gatatgatgc
     2821 ccgccgacta ctacgcggac ggcaaggcgc agtatgtgca ggcgctgcaa aatcagttgc
     2881 aaatgtatag ccccgatggt ctcatgccgg cgggcggccc gcagtttgtg ctccagaccg
     2941 agaacgaaac caacttcgcc gtgaaggggc agagcatcga tctgagcgca acctatacca
     3001 acgagttcgt caacaatgcg ccgtaaatat tacggacggg gggcggcgaa acggtatcct
     3061 acgccaggtt cggtgagcag gtagcgcggg ttggcgggat caggttcaag cttctggcgc
     3121 aggcggttga cgtacacgcg caggtactcg gcctcgccac cgtattccgc gccccagata
     3181 ctttgcagca gcatgcgatg agtgagtact ttgccggcgt gcaagatgag ctgtttgagg
     3241 acggcaaact ccgtgggcga taatcgcact tccgccccgc gcaggcgcac gatgtggccg
     3301 gataggtcaa tctcaagctc gccgaactgt ttgatgtcgc cgctgggctg cggtatgtcc
     3361 cattgggtgc ggcgcaggac ggcgcgcaca cgggccagca gttcttccac cccgaaaggc
     3421 ttggtcaggt agtcgtccgc gccgagagcg agggctgtca ccttgtcgct ctccgcgtcg
     3481 agcgctgtca agacgatgat gggaacagcg gagtgttcgc gcacacgccg gcagacttcc
     3541 aggccatcca tgcggggcat catgatatcg aggatcagca ggtggggatg ttcctgttcc
     3601 cacgtggcca tcgcctctaa gccgttgctg gcttccaaca catcgaagcc gcgcgcgcgc
     3661 aggttgcgac tgacgaaatc gcgcagggcg agttcatctt ccgcgagcaa tatgcgtttg
     3721 gtcctggtcg tcatagtatc cctctttctg cgtcgaaagc tccgcgcgtg tgatgatgaa
     3781 gtttaaataa t
//
LOCUS       DASZAK010000611         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_86988, whole genome shotgun sequence.
ACCESSION   DASZAK010000611 DASZAK010000000
VERSION     DASZAK010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86988"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VGT44_10710"
     CDS             <1..1110
                     /locus_tag="VGT44_10710"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEV2581310.1"
                     /translation="NNNTFLYNTGPITSLDSANWNRRQFYTVTKVWDGGSTVLASGLA
                     SPPCNIGPFSTPNYASLAQAAIHQLSSGETVFAGQRAEGFYVDLGSIFDLGDLRPFQN
                     LHIEPRAAANGVNGTNNLNVHSIAIQVPIKALTEDGNVPTKVSDDDAVLGIWTSAYRQ
                     KVEMNDGSGDSSSVGPWMQVSRLGNPLFNEVIVPMAKKNLWNSLPPSSDSEFAQYVEH
                     PELAGLLPILYPGVFPHLAALTAARADLAAILLTGIPTGIISGFQNFTGTTQADMLRL
                     NVAVPPSSKANIYGILGGDLAGFPNGRRVQDDTVAIELRAIAGVTYPLVAPSYKADGA
                     AALVTDGLTPASVNAPFLSSFPYLGTPYDGYDVPG"
     gene            1320..2201
                     /locus_tag="VGT44_10715"
     CDS             1320..2201
                     /locus_tag="VGT44_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581311.1"
                     /translation="MERTRAFYEQAHTEQAVLDIGQDIGALVVYTGGELRGREIEVSP
                     KGNETKRTHTAILERRVNGRVIFAGLFAALPAGDYTIWMDPTSDVTISGGQVAEIDCR
                     HCSNIYAPYAFHSRTLEEDTSRQSINALLPPRYRDGRPVSSAPMGSAPMRYAGDGQVA
                     WNKMWTDYCDLALAGGPPHRDTLLEPVPPQDVKADLDSYRRVVAEVERGLRLVTGLPV
                     VPSAQLGWVGLQCASEEMALWLLRAIIVENVCVRREGTILYLPVGPSFLLEKEIKNVI
                     TVVAKTNHYWQEHLSAS"
     gene            2317..>2861
                     /locus_tag="VGT44_10720"
     CDS             2317..>2861
                     /locus_tag="VGT44_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2581312.1"
                     /translation="MSHTNIKVYGTNWCSDCKRTKKFLGEQRVHYEFIDIEEDMEGQA
                     FVQKAQNGGLTIPTVVFEDDSILIEPSNAEVAAKLGLETKAKCEFYDLIIVGGGPTAL
                     TTAIYAARDGYDVLVIERSGLGGQAGITERLDNYPGFPQGVTGAELAERVIEQAKRYG
                     VELLSAQNVIGVANDLEDHVVS"
BASE COUNT          612 a          895 c          812 g          542 t
ORIGIN      
        1 aacaataata cctttctcta taacaccggt ccgatcacct cgctggatag cgccaactgg
       61 aaccgccgcc agttctatac cgtgacgaag gtctgggatg gcggctcaac ggtgctggcg
      121 agcggcctgg ccagcccgcc ctgcaacatt ggcccgttct ccacgcccaa ctacgcgtcg
      181 cttgcccagg ctgccattca ccagctttcc agcggcgaaa cggtcttcgc cggccagcgc
      241 gccgagggct tctatgtaga cctgggttcg atcttcgatc tcggcgacct gcgcccattc
      301 cagaacctgc atatcgaacc acgcgcagcc gccaatggcg tgaacggcac caacaatctg
      361 aatgttcact ccatcgccat tcaagtaccc atcaaagccc tcacggaaga tggcaatgtc
      421 cccacgaaag tcagtgatga tgacgcggtg cttggcatct ggacttccgc ctatcgccag
      481 aaggtcgaga tgaacgacgg atcgggcgat tcgtcgagcg tcggcccctg gatgcaggta
      541 tcgcgacttg gcaacccgtt gttcaacgaa gtcattgtgc caatggcaaa gaagaacctc
      601 tggaactcct tgccgcccag cagcgacagc gagtttgccc agtacgtgga gcatcccgaa
      661 ctggcagggc tgctgccgat cctctatccg ggcgtcttcc cgcacctggc ggcgctcacc
      721 gccgcgcgcg ctgacctggc agccatcctg ctcaccggca ttccaacggg gatcatttcc
      781 ggcttccaga atttcaccgg gactacccag gcggatatgc tgcgcctgaa tgtagccgtg
      841 ccgccgtcat cgaaggcgaa catctacggc atcctgggcg gggacctggc cggtttcccg
      901 aatggccgtc gtgtgcaaga cgacacggtt gccatcgaac ttcgcgccat cgccggtgtc
      961 acctatcccc tggtcgctcc cagctacaag gccgacggtg ccgccgccct cgtcacagat
     1021 ggcctgacgc ccgcaagcgt caacgccccc ttcctatcga gtttccccta cctgggaact
     1081 ccctacgacg gctatgacgt gccaggctag tcgatacgtg ccggttgacc gcgcccaacg
     1141 ccgatgatga acgttcacaa tcaagatcgg taagaagttg tcatcctgag cgcagcgaag
     1201 gatctcactc gccggacacc gagatccttc gctgcgctca ggatgacaga accgctatag
     1261 gcgcaacatg cctttgccgg agtcacgcat atatatatat cagaaaggcg atcgttctga
     1321 tggagaggac cagagcattt tacgaacagg cgcataccga gcaggcggtg ctggatatcg
     1381 ggcaggatat cggggcgctg gtcgtctata cgggcggaga gctgcgcggc agggaaatcg
     1441 aagtcagccc caaaggcaac gagacgaaac gcacgcatac cgctattctg gagcgcaggg
     1501 tcaacgggcg cgtgattttc gcgggtcttt tcgccgcgct tcctgccgga gactacacca
     1561 tctggatgga ccctaccagc gacgtgacca tctctggcgg ccaggtcgcc gaaatcgatt
     1621 gccggcactg ctccaacatc tacgcgccct atgcttttca ctcccgcacc ctggaagagg
     1681 atacgtctcg ccagtccatc aatgctctgc tcccaccgcg ctatcgcgat gggcgacccg
     1741 tcagctccgc cccgatgggg tcggctccca tgcgctacgc cggcgatggg caggttgcct
     1801 ggaacaaaat gtggacggac tactgcgacc tggcgctggc aggcggccca ccccatcgcg
     1861 atacgctgct ggagccagtt cccccgcagg atgtcaaagc cgacctggac tcttaccggc
     1921 gcgttgtcgc cgaagtagaa cgcggcctgc gcctcgtcac cggcctgccc gtcgtgccca
     1981 gcgcgcaatt gggctgggtc ggcctgcaat gcgcaagcga ggagatggcg ctctggctgc
     2041 tgcgcgccat cattgtcgaa aatgtctgtg tgcggcgtga agggactatc ctctatcttc
     2101 ccgtcgggcc atccttcctg ctcgaaaaag agatcaaaaa cgtcatcacc gtcgtggcga
     2161 aaaccaatca ctactggcaa gaacatctct ccgcttcata aacggcaaag taacgttaca
     2221 aagaccccgg cgttgcagcc atcggggtct ttgtaacgtt actttgcgtg actatactat
     2281 accagaaaaa gcgaaacagt ttgaaaggaa gggtctatgt cacatacaaa tatcaaagtc
     2341 tatggcacca actggtgcag cgattgcaag cgcaccaaga aattcctggg ggagcagcgc
     2401 gttcattacg agttcatcga tatcgaagag gatatggagg ggcaggcgtt tgtgcagaag
     2461 gcgcaaaatg gcggactgac gattccgacc gtcgtcttcg aggatgactc aatcctgatc
     2521 gagccatcaa acgctgaagt ggccgcgaaa ctcggcctgg aaacgaaggc aaagtgcgaa
     2581 ttctacgacc tcatcatcgt cggcggcggc ccgaccgcgc tgaccaccgc tatttatgcc
     2641 gcgcgcgatg gctatgatgt gctggtgatc gagcgcagcg gcctgggcgg gcaggcaggc
     2701 atcaccgaac ggctcgataa ctaccccggc tttccccagg gcgtcaccgg cgcggaactc
     2761 gccgagcgcg tgatcgagca ggccaaacgc tacggcgtgg agttgctttc agcgcaaaac
     2821 gtgatcggcg tggcgaatga tctggaagac cacgtcgtct c
//
LOCUS       DASZAK010000612         2046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_690886, whole genome shotgun sequence.
ACCESSION   DASZAK010000612 DASZAK010000000
VERSION     DASZAK010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2046
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690886"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1399
                     /locus_tag="VGT44_10725"
     CDS             <1..1399
                     /locus_tag="VGT44_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV2581313.1"
                     /translation="NRHLRITYTRILICGETRKRMKSSGIIDPDELVEAQPSTRRRFL
                     LTRGSAVIGILLLLAIVFNATSAGYAQFLGGQGWGIVLGGPGASSEQALLNTIRNKLR
                     QQATPGATATAQQQETAQQYINLILQGMTLDQKLGQMMIVQFLGPTYTLAINTMISQY
                     DVGAALLYSANGNIQSKTQLKGLIAQMQQASGLSMLISIDQEGGYVDRLVNLDGPRPS
                     AASIGATNDPAQALVEGERDAADLSSYGFNLNLAPVVDVTTVPNPQLFGRTFGDTPDS
                     VTRMAAAYLQGLQQSGKVFGTLKHFPGLGDVATDPHNGVPDVYRSLAALQAIDWAPYR
                     ALIAQGNVHAVMVTHEVIHAVDPNLPSSLSYKLVTGILRDQLGFNGVIMTDSLTMQGL
                     TAFYSEAQAAALAIEAGDDLLMGASTPQDVAAMIEGIKQVIASNGISMARIDDSVRRI
                     LTLKFELGLLPLPNN"
     gene            1611..>2046
                     /locus_tag="VGT44_10730"
     CDS             1611..>2046
                     /locus_tag="VGT44_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581314.1"
                     /translation="MIETDRQDTPDAPETQAAPDAPTAQSAQTIPVVQTTQSAQNDAQ
                     QKRMSYIMLGLVALQTLLVTLALVPPQFWTRFLPGSPTASLDGPFPVALAPIISALLY
                     VIPSTIGFLNKSWQRALLYATIPAWIGLGLFVIAASLKIGAFN"
BASE COUNT          434 a          633 c          580 g          399 t
ORIGIN      
        1 aaaccggcac ctacggatta cttatacccg tattttgatc tgtggcgaaa cgaggaagcg
       61 catgaaatct tccggcatta ttgatcctga tgagcttgtg gaagcgcagc catcgacgcg
      121 gcggcgcttc ctattgaccc gcggctcagc cgtgatcggc atactattgc tgctcgcgat
      181 tgtatttaac gctaccagcg caggctacgc gcagtttctg ggcggacagg gctggggtat
      241 cgtgcttggc ggccctggcg cgtcgagcga gcaggcgctg ctgaacacca ttcgcaacaa
      301 attgcgccag caagcgacgc ccggagccac ggctaccgcg cagcagcagg agacggcgca
      361 gcaatacatc aacctgattc tccagggcat gacgctcgat cagaagctgg gacagatgat
      421 gattgtacag tttcttgggc cgacctacac gcttgccatc aacaccatga ttagccagta
      481 cgacgttggc gcagcactgc tctatagcgc aaacggtaac attcagagca agacgcagct
      541 caaggggctg atcgcccaga tgcagcaggc cagcggccta tccatgctga tctccatcga
      601 tcaagagggc ggctatgttg accgcctcgt caacctggac gggccgcgtc catcggctgc
      661 ctccattggc gcgacgaatg acccggcgca ggcgctggtt gagggtgagc gcgacgccgc
      721 cgacctgtcc tcctacggtt tcaacctgaa cctcgcgccg gtcgtagatg tcacaactgt
      781 gccgaacccg cagttattcg gacgcacctt tggcgacacg cccgacagcg tgacgcgcat
      841 ggcggcggcc tacctgcaag gcttgcagca gagcggcaag gtcttcggta cgctcaaaca
      901 ttttccaggg ctaggcgatg tcgccaccga cccgcacaac ggcgtccccg acgtgtaccg
      961 ctcgcttgct gcgctgcaag ccatcgattg ggccccctac cgcgcgctga tcgcgcaggg
     1021 caacgttcat gccgtgatgg tcacacacga ggtgattcac gccgtcgatc ccaatctgcc
     1081 ctcgtcgctc tcctataaac tcgtgacggg catcctgcgc gaccaactcg gcttcaacgg
     1141 cgttattatg accgacagcc tgaccatgca aggcttgacc gccttctatt ccgaagcgca
     1201 ggcggcggcg ctggcaatcg aggccgggga tgaccttctg atgggcgcat cgacgccgca
     1261 ggatgtcgcc gccatgatcg aaggcatcaa gcaggtgatc gcgtccaatg gcatcagcat
     1321 ggcgcgcatc gatgactccg tgcggcgcat cctgaccctg aaattcgagc tagggctgct
     1381 gcccctgccg aataattaac gctcttgtcg gagggacgcg atgatgaata tttatgaacg
     1441 aagtcgggaa tgtcaccctg agcgaagcga agggtctcgg tcgaggggcc acgagatgct
     1501 tcgcttcgct cagcatgaca gagccgactt tggttgagaa agttcatcat tgcatcacta
     1561 caaccgaggc catttttgtt cgtcaaaaaa attgagggaa agacacggct atgatcgaga
     1621 cagataggca agacacgcca gatgcgcctg aaacacaggc agcgccggat gcgccgaccg
     1681 cacaaagcgc gcaaactata ccggtagtac agactacaca aagcgcacaa aacgacgcgc
     1741 aacaaaagcg catgagctac atcatgctgg gcctggttgc cttgcagacc ctgctggtga
     1801 cgctggcgct cgtgccgccg cagttctgga cgcgcttcct gcctggttcg cccacagcct
     1861 cgctagatgg gccatttccg gtagcgcttg ctcccatcat ttccgcgctg ctttatgtga
     1921 tcccctccac gattggcttc ctcaataaaa gctggcagcg cgcgctgctc tacgccacca
     1981 tcccggcctg gatcggtctt ggcctgttcg tgattgccgc ctccctcaaa atcggcgcgt
     2041 tcaacc
//
LOCUS       DASZAK010000613         5855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_513571, whole genome shotgun sequence.
ACCESSION   DASZAK010000613 DASZAK010000000
VERSION     DASZAK010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513571"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..370
                     /locus_tag="VGT44_10735"
     CDS             <1..370
                     /locus_tag="VGT44_10735"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2581315.1"
                     /translation="GESGYFMQPAVLADVNNHMRVAREEIFGPVVCIIPFDGEEDALR
                     IANDTPYGLSGSVWTQNLGRALRVAKGMRAGVLSVNSSHSVHTEAPFGGYKQSGFGRE
                     MGMHAAQLYTEVKSVFFSQE"
     gene            484..1440
                     /locus_tag="VGT44_10740"
     CDS             484..1440
                     /locus_tag="VGT44_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581316.1"
                     /translation="MAKQASNQLSPDQQQPYDTLLKSILEGQEREILPYFFPRVRYLE
                     TLNVEAVRTPLRVDRVYKVKYKERVCILHIEFESGPNNNMADRLLDYHAYLHRKYKQP
                     VLSIIVYPFPSKMAEPPLREVNGDGEILIFHFWVLPLWKLRAEQYLSEHAVALYALLP
                     TMEGANAQLLHKAVDEMVEYYQGDDTRLVQELRWMGIVLRRAKMPRREKRAIEERLTM
                     WDDLFERDPKMRKIRKESEQKGLVEGEALGLAKGEALGLAKGLQKAIITAIKLRYPPL
                     IEIAQERIPQIDKPETLNVLLEQISTVPDEATVRMLLDLIAA"
     gene            complement(1626..4058)
                     /locus_tag="VGT44_10745"
     CDS             complement(1626..4058)
                     /locus_tag="VGT44_10745"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEV2581317.1"
                     /translation="MQRIPLRKLITIGVTLVLLSVSILSVVVIARARQNAPYLMTGQV
                     APLVSRAHLVGPAGAQQQLNLSVGLQLRNQQELASLLSDLYNPRSPSYHHFLTAQEFA
                     AEFDPTADQVQQVETYLQQQGLAITHVASNGLLIDANATVAQAESAFQVTINNYVLGS
                     NAFFANANPPTIPGALSSLINSIGGLDNSVKMHPLYQLSSHPHGKTKPAVIGAKQKPH
                     ATQSGYGPTDLSGAYDAKSLQTTSLSGSNQTVAVFELDGYTSSDITAYLQNYSLGTPN
                     ISNVLVDGFNGAAGAGAIEVDLDIEVVAAMAPKANQIVYEGPNTTQGVNDTYNKIVTD
                     NKAQVMTTSWGECEAQSGNSELQTLDSIYAQGSAEGIAMYAAAGDSGAYDCNDTNLAV
                     DNPADDPNVTGVGGTNLQISSGAYGSESVWSNASDTSRSPQGAGGGGGLSSYFKLPTW
                     QTGPGVQNQYSNGNRQVPDVTADADPASGYAVYCTSTAAGCSSSGWIVVGGTSAAAPM
                     WAGSTALINGYLQSQNKSRLGFANPTLYALASSTQPFAPFHDITSGTNLFYPATAGYD
                     EASGLGSPDVYNIARDVAAGVVSPPPTPTPSPTGTTTPTPSPSPTGTPPPSPTSTTTP
                     FPSPTVTTTPVPPPTFTATPPPSPTPTSGNLIQNGDFEQGPAVWQESSSGGYELITST
                     NPHSGNYSAYLCGYSSCSDSISQDFTVPSGASSLTIDYWWFGDTNHTTSKCVDSFTVS
                     VVDSSGNTIAQVQTACNTDANSTWQEQQFDASSLLANYAGQTVTLVFAAQSSSSKATS
                     EFFVDDVVVSGA"
     gene            4401..4898
                     /locus_tag="VGT44_10750"
     CDS             4401..4898
                     /locus_tag="VGT44_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581318.1"
                     /translation="MMADYSYENSLEWTQREYPSNDHYTEFTAYIDYPMPPPPVGQLS
                     VQPRPQRASQAQQRPQAQRQPSQDKARLKRMSKGEALDLLESLKKTILAGALIGFFVL
                     SALVATHMVGSAANNTPSPDPSFQQVPSDNGNGFFNQQGSGSSGGGFQFGGGGGQPSS
                     GTRVS"
     gene            4919..>5855
                     /locus_tag="VGT44_10755"
     CDS             4919..>5855
                     /locus_tag="VGT44_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV2581319.1"
                     /translation="MNTLWQSVTWDVARAGGFTAYILLALAVAIGLALTMQLQSPSIW
                     PRIVNSELHNFVTLLALVFTTIHVLAVTIDPFTNFGLNEVLIPFVSHYRPIWMALGIV
                     ALYLGLAIGLSTWVRPLIGYSWWRRLHVLTLVVFALVTVHGIATGSDTQSWWGFGIYA
                     ACIALIAGLLIRRLLVPANQRGRKHPVFASATGLATLATLAFMIIGPMRPGWNALAGG
                     TSSASAASATQGATSGDPFASSFAASLQGTMTQSGPDASGITTLNVHTTLSNGAQGVL
                     TIVLQGAHLTGGDDGLSISSTSVTLGTSSASQLYQG"
BASE COUNT         1228 a         1584 c         1741 g         1302 t
ORIGIN      
        1 cggcgagagc ggctacttca tgcagcccgc ggtgctggcc gatgtcaata accacatgcg
       61 cgtggcgcgc gaggagattt tcggcccggt ggtctgcatc atccccttcg acggcgaaga
      121 agacgcgctg cgcatcgcca acgatactcc ctacggcctg tccgggtcgg tctggacgca
      181 gaacctgggg cgcgctttgc gtgtggcaaa gggcatgcgc gccggcgtcc tatccgtcaa
      241 ctccagccac agtgtccata cggaagcgcc ctttggcggt tataagcaaa gcggctttgg
      301 ccgcgaaatg gggatgcacg cggcgcagtt gtacactgaa gtgaaaagcg tgtttttctc
      361 gcaagagtag gccgcaaaag ggatttggcg caacctctcg catcagtcaa taatttacta
      421 ttgcttgctc agcttagcta tgcaataatc cagcaaatca agtggaaagg gtgttcaatg
      481 agcatggcta agcaagcatc aaatcagctt tcaccggatc agcagcaacc ctatgatacc
      541 ctcttaaaat cgattctgga aggccaggag cgtgagatac taccctattt tttcccaagg
      601 gtgaggtatc tggaaacgct aaacgttgaa gcggttagaa ccccgctacg tgttgatcgc
      661 gtctataaag tcaagtacaa agagcgagtt tgtatcctgc acatcgagtt tgagtcaggc
      721 cccaacaaca atatggcaga ccgtttgttg gattatcacg cttatctgca tcgtaaatat
      781 aagcagcccg ttttatcgat catcgtatat cctttcccgt caaaaatggc tgaacctcca
      841 ctgcgcgaag taaatggcga tggagagatt ctaattttcc atttctgggt cctgcctctt
      901 tggaagttgc gagccgaaca atatctcagc gaacacgcag tcgctctgta cgccttgttg
      961 cccacaatgg agggcgcaaa cgcgcaactg cttcacaaag ccgtggatga gatggtagaa
     1021 tactatcagg gtgatgatac taggttagta caggaacttc gctggatggg tatcgtatta
     1081 cggagggcaa aaatgccgcg tagggagaaa cgagcaatag aggagcgact gactatgtgg
     1141 gacgatcttt tcgaacgaga cccgaagatg aggaagattc gcaaagaaag tgaacagaaa
     1201 ggacttgtcg aaggcgaagc tctggggtta gccaaaggcg aagctctggg gttagccaaa
     1261 ggtttacaga aagcaatcat aacggctatc aaattgcgtt atccgccttt gattgagata
     1321 gcccaggagc ggattcctca gattgataaa ccggagacgc tcaatgttct actcgaacag
     1381 atcagcacgg tgccggatga ggcgacagtg cggatgttgc tcgatttgat cgcggcctga
     1441 cctgggtgga gagacaatcg ccgttatcag ctcacattga caatcgcctt tatatctact
     1501 atattatctc cagccttcgc tactctgtac acctttatta ggccattacg agcctgcacg
     1561 tctagagagc gaaggcgaaa ggcgagaccg gacaacctgc ggttgtccgg ctcgtccgtt
     1621 ttgggctatg ccccgcttac cacgacatca tcgacgaaga attcgctggt agccttcgac
     1681 gaggaggact gggcagcgaa gacgagcgtc accgtttgcc cggcgtagtt cgcgagcagg
     1741 ctgctggcat cgaactgctg ctcctgccag gtcgagttag cgtcggtatt gcaggcggtc
     1801 tgcacctgcg cgatggtgtt gccactgctg tcaaccaccg atacggtaaa gctgtccaca
     1861 cacttcgagg tagtatggtt ggtgtcaccg aaccaccagt agtcaatggt cagcgaagaa
     1921 gcgccactgg ggacggtgaa gtcctggctg atgctatcgc tgcaactgct atagccacac
     1981 aggtatgcgc tgtagttacc gctgtgcgga ttggtcgagg tgattaactc gtatccgcct
     2041 gaggaagact cctgccagac cgcaggtccc tgctcgaagt cgccattctg gatcaggttg
     2101 ccggatgtcg gcgttggcga cgggggcggc gttgccgtga aggtcggcgg cggcaccggc
     2161 gtagttgtga ccgtcgggct ggggaacggt gtcgtcgtac tcgtcgggct tggtggcggc
     2221 gttcccgttg ggcttggcga cggcgtgggt gttgtcgtac ccgtcgggct tggtgtgggg
     2281 gttggcggtg gcgagaccac accggctgct acgtcgcgcg cgatattgta gacatcgggc
     2341 gagccgaggc cgctggcctc gtcataccct gccgtcgcgg ggtagaacag gttcgtgcca
     2401 ctggtgatat cgtggaacgg cgcaaacggc tgcgtggaac tcgccagtgc atataacgta
     2461 ggattggcga agcccaggcg cgatttattc tggctttgca ggtagccgtt gatgagcgcg
     2521 gtgctgcctg cccacatggg cgcggctgcg cttgtgccgc caacgacgat ccaaccagat
     2581 gatgagcaac ccgccgccgt ggaggtgcag tacacagcat agccagaggc gggatcggca
     2641 tccgccgtga catcaggcac ctggcggttg ccgttgctgt actggttctg cacgccgggg
     2701 ccggtctgcc aggtcggcag tttgaagtag ctgctgaggc cgccaccacc gcccgcgcct
     2761 tgaggactgc gcgaggtatc ggaggcgttg ctccagaccg actcgctgcc gtaagcgcca
     2821 ctgctgattt gcagattggt gccgccgacg ccggtcacgt tgggatcatc ggcggggtta
     2881 tcgacagcga ggttagtgtc gttgcagtca tacgcgccgg agtcgccagc cgccgcgtac
     2941 atggcgatgc cttcggcgga accttgagcg taaatactgt cgagcgtctg tagctcggaa
     3001 ttgccgctct gcgcctcgca ctcgccccaa cttgtcgtca tgacctgcgc tttgttgtcc
     3061 gtcacgattt tgttgtacgt atcgttgacg ccctgcgtcg tattggggcc ttcatagaca
     3121 atctggttcg ccttgggggc catggccgcg acgacttcga tatccaggtc gacttcgatc
     3181 gcgcccgcgc ccgccgcgcc gttgaagcca tctaccagca cgtttgagat gttgggcgtc
     3241 cccagactgt agttttgcag gtaggcggtg atatcgctgc tcgtgtagcc atccagttcg
     3301 aagacggcca ccgtctggtt gctccccgac agactggtgg tctgcaagga tttggcgtca
     3361 tacgcgccgc tcagatcggt cgggccatag ccgctctgtg tcgcgtgtgg cttctgtttg
     3421 gcgccgatca cggcgggctt ggttttgcca tgcggatggc tactcaactg gtagaggggg
     3481 tgcatcttga cactgttatc cagaccgcca atcgagttga tgagcgatga aagcgcgcct
     3541 ggaatggtgg gaggattggc gttggcgaag aaggcattcg accccaggac gtagttgttg
     3601 atcgtcacct ggaaagccga ttctgcctgg gccactgtgg cgttggcatc gatcagcagg
     3661 ccgttcgacg caacatgcgt gattgccagg ccctgctgct gtaaataggt ttcgacctgc
     3721 tgtacctggt cggcggtcgg atcaaactcg gcagcaaatt cctgcgcggt gagaaagtga
     3781 tgataacttg gagatcgggg gttatacaag tcgctcaaca ggcttgccag ttcttgctga
     3841 tttcgcaact gcaacccgac tgagagattg agttgctgtt gagcacctgc gggtccaacc
     3901 aggtgcgcgc ggctgacaag cggcgcaacc tggcctgtca tgagatatgg cgcattctgt
     3961 cttgcccgcg caatcacaac gacgctgaga atactgacgc tgagcaggac gagtgtgacg
     4021 ccaatagtta tcagcttgcg taaggggata cgctgcatat aacactccac tctactatga
     4081 aaatactaag ggtacgacgt tttgacatac cttgataaac ggggaaacag gtgagcttgg
     4141 gggcatttgc atcaacatta aagctctctc atgtgatctt tacacttttt ttacacattc
     4201 ctgttccttt tctcatgact acgctctatg ctttatttga actgttattg tgtcgcaacg
     4261 accgtagggg cgagccttta tggtcgcccg cccgtggaca cacagaatca tctacaccat
     4321 gagaaacggc aaaataatga cgggcgacca taaaggctcg cccctacggt cggaatatag
     4381 aagaagcata tccggagcaa atgatggcag actattcgta tgagaattcg cttgaatgga
     4441 cacaacgaga atatccgtcg aacgatcact atacagaatt caccgcttat atcgactatc
     4501 ctatgccgcc tcccccagta ggtcagctat cagtacaacc caggccgcaa cgcgcatcac
     4561 aggcacagca acgaccacag gcgcagcgac aaccttcgca ggataaggca cgcttgaagc
     4621 gcatgtcgaa gggtgaggct ttagacctgc ttgaatcctt gaaaaaaact attctggccg
     4681 gcgcgctgat aggcttcttt gtcctcagtg cgctcgtagc aacacacatg gtgggcagcg
     4741 cggccaataa cacgccgtcc cccgacccct cgttccagca ggtgccatca gataacggca
     4801 atggcttctt caaccagcaa gggagtggca gcagcggcgg tgggtttcaa tttggtggcg
     4861 gcggcggaca gccatcgagt gggactcggg tatcctgaag catgatagtg gagggaacat
     4921 gaatacactc tggcagtctg tgacctggga tgtcgcacgg gcgggcgggt ttaccgccta
     4981 tatcctgctg gcgcttgcgg tggcgatcgg gctggcgctg acgatgcagt tgcaatcacc
     5041 cagcatctgg ccgcgcattg tgaatagcga attacataat tttgtgacac tgcttgcgct
     5101 cgtcttcacg acaatccacg tgctggcggt gacgattgat cccttcacga acttcggttt
     5161 gaacgaagtc ttgatcccct tcgtgagtca ctatcgacca atatggatgg cgctgggcat
     5221 tgtcgcgctc tacctgggtc tggcgattgg tctcagcacc tgggtgcgtc cgttgatcgg
     5281 ctatagctgg tggcggcgtt tgcacgtgtt gacgctggtc gtgttcgcgc tcgtcaccgt
     5341 gcatggcatc gccaccggca gcgatacgca gtcctggtgg ggatttggca tatacgcagc
     5401 ctgcatcgcg ctgattgcgg gcttgctcat caggcgtttg ctcgttccgg ccaaccagcg
     5461 cggacgcaag catcccgtgt tcgcctcggc gacgggcctg gcgacgctcg cgacgctggc
     5521 cttcatgatt atcgggccga tgcgccccgg ctggaacgcg ctcgccggag gcacgagttc
     5581 tgcctcggct gccagcgcga cgcaaggcgc gacctccggc gatccattcg cctcgtcctt
     5641 tgccgcctca ttgcaaggaa cgatgacgca gagcggtccc gatgccagcg gcataacgac
     5701 cctgaacgtg catacgaccc tgagcaatgg cgcacagggg gtcttgacga ttgtcctcca
     5761 gggggcacac ctcaccggtg gtgatgacgg cctttcaatc agttccacca gcgtgacgct
     5821 cggcaccagc agcgcctccc agctctacca ggggc
//
LOCUS       DASZAK010000614         3550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_121512, whole genome shotgun sequence.
ACCESSION   DASZAK010000614 DASZAK010000000
VERSION     DASZAK010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121512"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..492)
                     /locus_tag="VGT44_10760"
     CDS             complement(43..492)
                     /locus_tag="VGT44_10760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581320.1"
                     /translation="MQTTRFTLSKYICGMVASLIIFLAGGWLILAPFALGYQPYGADW
                     ASQTTNDFATGIPVAVVALIAFAFFFLSLLGGLRAAGVLAARQRPQAGVPVMTQAPNS
                     AQQATAQQSDLDKTLTTLAAALAADLSARRQPGGNQQTDRSIEQMTK"
     gene            complement(543..2489)
                     /locus_tag="VGT44_10765"
     CDS             complement(543..2489)
                     /locus_tag="VGT44_10765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEV2581321.1"
                     /translation="MAQTQAGSTTTIIQEPQALSAIVSRQRLISILIGVLLGMLLAAL
                     DQTIVGTALPRIVSNLGGLDHYAWVVTAYLLASTVSVPIYGKLSDIYGRRFFFITGMV
                     IFLAGSALAGTSQDMTQLIIYRGIQGLGAGAMMPIALAIIGDLFPPSERGKWQGLFVA
                     VFGLASIIGPTLGGWITDNWGWRWVFYVNMPVGAIAILTAGFVLPKAVRRIQHTIDYL
                     GAITLVAGAVPLLLAFSWAGTQYAWGSWQVILMFVIAAVMLAVFLLLELRNTEPIISP
                     RLFKNSIFLISVVSTFLVSAGMFGAILYLPLFVQGALGNSATNSGYVLTPLMIGFMIS
                     SILGGQLLSRTGRYKVLALVGFAVAALGMFLLSRMEATTGEGEVVRNMIVTGLGIGVM
                     MSLFTIVVQNAFPYRQLGEVTATLTFFRSIGSTIGVAVMGSIMTNNLQSSLQSNLPAA
                     LTRLGPASGLSQLNPQALLDPSQLGQLQHSFARFGAQGIILFHQFIEAVRVSLASAIT
                     QVFFLGFIIMLLGLVVCLFLREIPLRKSHNAQAAQQATTGPNRSRALLGLTLALMARE
                     AQKPEADPQILATLSNAVNGRYPHTWSDEERGRAVAQDIIEPLSITLLASSVSNGRAT
                     GLAQTTEGTPPEASNTPSSSAILG"
     gene            complement(2520..3008)
                     /locus_tag="VGT44_10770"
     CDS             complement(2520..3008)
                     /locus_tag="VGT44_10770"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2581322.1"
                     /translation="MDQTREALEREVLEQYQALGRAFKATAEPVWMEIDLTIAQLRTL
                     LVLAEEGPLVIGQIARRLGIGLSTGGHLVDRLVQAGLAERAEDAGDRRRTMARLTPAG
                     EDLYARLLNRTQHLQSLLQRMREGELTALLQGLKAMRRAVEEENEEKCAARTEFDQKS
                     SN"
     gene            complement(3254..>3550)
                     /locus_tag="VGT44_10775"
     CDS             complement(3254..>3550)
                     /locus_tag="VGT44_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581323.1"
                     /translation="QTEPAQHVHTFAAELTASAPNRSKALLGLTLALLAQEAQLPEAD
                     PQILATLSSAASGRYPQTWSEEQVGRAIARDLLEPLSIELLASSTSNGRGHARE"
BASE COUNT          704 a         1066 c         1047 g          733 t
ORIGIN      
        1 cagtcgcctc attgcatttc ttgcactcat cacgcatctc tcctatttcg tcatttgttc
       61 gattgaccgg tcggtttgtt gattaccgcc cggctggcga cgcgcgctca aatcggcggc
      121 gagcgccgcc gcgagtgttg tcagggtctt atccaggtcg ctttgctgcg ccgttgcctg
      181 ttgtgctgaa ttcggcgcct gggtcatgac tggtacccct gcctgtggtc gctgacgggc
      241 cgcgagtacc ccggcagccc gtaagccacc cagcagcgag aggaaaaaga aggcgaaggc
      301 gatcagtgcc accactgcga cgggtattcc cgtcgcgaag tcgttggtgg tctggcttgc
      361 ccagtccgcc ccgtagggct ggtagccaag cgcaaatggt gccaggatca accatcctcc
      421 ggccaggaag atgatgagac ttgccaccat accacaaatg tacttgctca aggtaaatcg
      481 tgttgtttgc atgtacgaaa cctccgtgtt atgtctattg gtttggaacg ggtgcctatg
      541 tattatccta atatggcact cgatgaaggg gtgttgctcg cctcaggtgg tgtcccctcc
      601 gttgtttgcg ccaacccagt cgcgcgccca ttgctaaccg aggaagccag cagcgtaatc
      661 gacaatggct cgatgatatc ctgcgcgact gccctgccgc gttcttcgtc actccaggta
      721 tgcgggtagc gtccattgac agcattcgag agcgttgcca ggatttgcgg atcggcctcg
      781 ggcttttgcg cctcgcgcgc catcaatgcc agtgtcaggc ccagcaaggc ccggctgcga
      841 ttcggaccgg tcgtcgcctg ctgggctgct tgcgcgttgt ggctcttgcg cagcggaatt
      901 tcgcgcaaga agagacacac gaccaggccc agcagcatga tgatgaagcc caggaagaat
      961 acctgcgtga tggcgctagc gaggctcacc cgcacggcct cgatgaactg gtggaagagg
     1021 ataatcccct gcgcgccaaa ccgcgcgaag ctgtgttgca gctgcccgag ctggctcgga
     1081 tccagcagcg cctgtggatt gagttgggac agaccgctgg cggggccgag tcgtgtcagc
     1141 gccgccggca aattgctctg cagcgaactc tgcaagttgt tggtcatgat ggatcccatg
     1201 accgccacac caatcgttga gccgatggaa cggaagaacg tgagcgtggc tgtaacctcg
     1261 cccagctgcc ggtacgggaa agcgttctgg acgacgatgg taaagaggct catcatcaca
     1321 ccaatgccca gtccggtcac aatcatgttg cgcacaacct cgccctcgcc cgtcgtcgcc
     1381 tccatgcgcg agagcaagaa catgcccagc gcagcgacgg cgaacccaac gagcgccaga
     1441 accttgtagc gcccggtgcg cgagaggagc tgacctccca ggatgctgct aatcataaag
     1501 ccgatcatga gcggggtcag cacgtagcca gaattggtgg cgctattgcc aagcgcgccc
     1561 tgcacgaaga gcggcaggta caggatggct ccgaacatgc ccgcgctgac caggaaggtc
     1621 gagacgaccg agatcaggaa gatgctgttt ttaaacaaac gcgggctgat gatcggctcg
     1681 gtattgcgca actcgagcag gagaaagacg gccagcatca ccgcggcgat gacgaacatg
     1741 agaatgactt gccacgagcc ccaggcatac tgcgtcccgg cccacgagaa tgccagcagc
     1801 aggggtaccg cgcccgccac cagtgtgatg gcgcccaggt agtcgatcgt atgctggatg
     1861 cggcgcacgg ccttcggcaa cacgaatccg gccgtcagga tcgcgatggc gcccaccggc
     1921 atattcacgt agaacaccca gcgccagccc cagttgtcgg tgatccagcc gcccagcgtc
     1981 ggcccgataa tcgaggcgag accgaagacc gctacaaaaa gtccctgcca tttgccgcgc
     2041 tcagacggcg gaaagaggtc gccgataatg gccagggcga tgggcatcat tgcgccagcg
     2101 cccaacccct ggatgccacg atagatgatc aactgcgtca tgtcctggct ggtgccggcc
     2161 agcgctgagc cggccaggaa gatgaccatg cctgtaatga agaagaatcg ccgcccatag
     2221 atgtcggaga gcttgccgta gatcggcacc gagaccgttg aagcgagcag gtaggccgtc
     2281 acgacccagg catagtgatc gaggccgccc agattggaga caatgcgtgg cagggctgtg
     2341 ccgacaatcg tctgatcgag cgcggccagc aacatgccca gcaacacgcc gatcagaatc
     2401 gagatgagcc gttgccggct gacgattgcg ctcagcgctt gaggctcctg gatgatggtc
     2461 gtcgtggacc cggcttgtgt ttgtgccatc agattacata cctcctctgt atcactctat
     2521 caattgcttg acttctgatc aaattccgtg cgcgccgcgc atttctcttc attctcttct
     2581 tctacggccc ggcgcatcgc ctttaagccc tgtaataagg cggtcagttc accctcgcgc
     2641 atcctctgta gtaaggactg caagtgctgt gtgcgattga gcagccgcgc gtagagatct
     2701 tcgcctgctg gggtcaatcg ggccatggta cggcggcgat ccccagcgtc ctcggcgcgt
     2761 tctgccaggc cggcctgcac caggcgatcg acaagatgcc cgcccgtcga tagcccgatc
     2821 cccaatcgcc gggcgatctg tccgatgacc aggggtcctt cctcggcaag cacgagcagc
     2881 gtgcggagct gagcgatcgt gagatcgatt tccatccaga ctggttctgc ggttgctttg
     2941 aacgcgcgcc cgagcgcctg gtactgctcc aacacctcgc gctcgagtgc ttcgcgcgtc
     3001 tgatccatgt tgttcctcct ttatctgctc agccgatact tcctttctgc agaagagtgt
     3061 accatattac tacgacacta tcaatctatt tgacgacgta ctttaaccgc acgctgtcgg
     3121 agtataaact gcttgtatcc cttataattg ctccacctct cgcatcaatt ccgctttttc
     3181 gacagaagcc tgggggttgg tgacctggtg gatgtagcgc actctgtcgt atcgtctatc
     3241 atggttttct ctcttactct ctagcgtgtc ctcgtccatt gctggtggag gaagccagca
     3301 gctcgatgga cagtggttca aggagatcgc gagctatggc tctcccgacc tgctcctcgc
     3361 tccacgtctg gggatagcgc ccactggcgg cggacgacag ggtcgccaga atctgtggat
     3421 cggcctccgg tagttgtgcc tcctgggcga ggagcgccag ggtcagacca agcagcgctt
     3481 tgctccgatt tggagccgac gcagtcagtt cggcagcgaa cgtatgaaca tgttgcgccg
     3541 gttctgtctg
//
LOCUS       DASZAK010000615         4762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_620637, whole genome shotgun sequence.
ACCESSION   DASZAK010000615 DASZAK010000000
VERSION     DASZAK010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620637"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1851
                     /locus_tag="VGT44_10780"
     CDS             247..1851
                     /locus_tag="VGT44_10780"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEV2581324.1"
                     /translation="MAQISQRLCPKCGTSLLANQRFCSNCGTTLDVIPGVAPDSAPGI
                     GSFPPSPPISMGNGQAPQLQMNQQVPSVQGTPSVPPHAPPQSLPGQPHAGQPGQFNQF
                     GQGNMPAQSAPPHGLAAHSQQPFPQQLPPHTPMPGQPMQPPSLPPQQPFHASAPAHMP
                     QSMPSSQAIQGQAPSAPSGAFHSPSAPSQAAQGQAAFHASAPPHMPPSQVPGGPAPHF
                     PAPQSIPAGSAARGAGSLAKRTARAGLHLAMKKPLISALIAVVIVGGGTGFAYYSYQS
                     TAPSSFVIATDRGNNQIDRIDIATRAVTVLLSNKVLPTTDSPDSIIFLNSTQMLIDFI
                     NSGEIGVGDIQQHTYKSLGKGHGSALRDMALRPDGSAVLISDAGGNILEYHTDTQKIT
                     PFAQNLGGVQGLAFDSNGNLFAAVGGQVIQLDPATGKQLKTYTLPGGSDGMAYDGRHK
                     VLDIAVGNTIISLDPATSKTATVIDGISIPDGLAIDRQGNLFIADDIGVLELNTNNQL
                     LIVGTNTNGITWDDVAPLSGNGAASY"
     gene            complement(1848..2387)
                     /locus_tag="VGT44_10785"
     CDS             complement(1848..2387)
                     /locus_tag="VGT44_10785"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2581325.1"
                     /translation="MLTVFYSPHATSIDNEAGRASGHADVPLSELGRQRARELGKHYE
                     TLAVEAVFCSDLQRAATTAEIAFSARGLPIVPDARLREYDYGTMTQYPVAQVEQEFAR
                     LVTEPFPGGESMVMVANRVEAFLRDVLQTYDGKSIVVIGHRATRYALEYWCEHTSLEE
                     LVRARWEWRDIPIWRYEFS"
     gene            3037..>4762
                     /locus_tag="VGT44_10790"
     CDS             3037..>4762
                     /locus_tag="VGT44_10790"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581326.1"
                     /translation="MPHHHAEPTHQLHISRHPTAPLHHQGTPQQDHPAQHPRHEQEPG
                     HEHEQAHHRAEHTGRPHHRLRLRSHGTHTAHAATASKVAVGTATKWLIVALTAVVVIA
                     TSGVVLVLASAPGLSLSGSSSSISSGGTLHIHGKGFLPGGSVTLTLDNGQHIAFQRNG
                     LAKSAGQGSVQEADVLFALDRAAASTNASIPVSVTGTFDADVTAPSSWSPGQHIIHAS
                     EGFGSRSADLQFTLLASPAKLVVTPTAIAFTAQAGTKESRSLQISNAGQATLTWTAAT
                     DGAKWLSLQGNGGQIGFNGQAAVMDVTADANHLKVGTYPATITVSSNGGNQQVAVALQ
                     VIQATGKKQAQLDVSPRTLDFGLLSPGQQLTQPLTVGNLGTAALLWHASAVNASWLSL
                     STSSGSLQPGAIPQAVQVTVNTNGLAAGSYAATITVASNAGTVQIPVAFDVLGTTPTP
                     LPPSPTAPPPPSPTTPPPPSPTAPPPPSPTTPPPPSPTAPPPPSPTVPPSPTAPPPTT
                     WSVSPTSLDLTNCTSGSGPTYMCFVTLTEGSSSPGNINWSVSDSLGTAGFSQSSGTLT
                     PGGSVQITI"
BASE COUNT         1042 a         1564 c         1227 g          929 t
ORIGIN      
        1 attcctgagt gcagaaactc cataatcgtc tatttatgtc gtatccatcc aggcatgggc
       61 gcggtattga ccatagtact gtaggactgg caaaatgaac atataaataa caaagtgttg
      121 tatatttata gtacaggtta ggtttcgttt ttcctctctt cagcgagcgg ttgtactcaa
      181 aaagctgggc agtccatcgt tctgatagtg aacgggcaat tatgcatcat cgaaaggtac
      241 ctggctatgg cgcagatatc tcaacgtctc tgtcctaaat gcgggacatc gcttttagca
      301 aaccagcgct tctgctcaaa ctgtggcaca acactcgatg ttattcccgg tgttgcaccg
      361 gattccgccc ccggcatagg aagcttcccg ccgtcaccac caatctcgat gggcaatggt
      421 caggcgcctc agcttcagat gaaccagcag gttccatcag tgcaggggac gccttcggtt
      481 ccaccgcacg cgccgccaca atcgctgccc ggtcaacccc atgctggaca gccgggccag
      541 ttcaaccagt tcggccaggg caacatgcct gctcaatcgg ccccgccgca tggattagcc
      601 gcgcatagcc agcagccgtt tccgcaacag ttgccgccgc atactcctat gcccggtcag
      661 ccaatgcaac cgccctcgtt acccccccag caaccgttcc atgcttccgc cccggcacac
      721 atgcctcaat ctatgccatc ttcacaggct atccagggtc aggcaccatc cgcgccatcg
      781 ggtgcgttcc acagcccatc tgctccatcg caggcggcac agggccaggc cgccttccat
      841 gcctcggctc cgccgcacat gccgccatca caggttcctg gggggccagc tccgcatttc
      901 cctgccccac agtccattcc tgcgggctca gccgcgcgag gggcaggttc cctggcgaag
      961 cgcactgcgc gtgcgggcct gcatctcgcc atgaaaaagc cgctgatctc ggcgctgata
     1021 gctgtcgtta tcgttggagg cgggacagga tttgcctatt actcgtacca gtcaactgcc
     1081 ccgagcagct tcgtgattgc caccgatcgc gggaataacc agattgaccg gatcgatatc
     1141 gcgacccgcg ctgtcacggt tctcttgagc aataaagttc tcccaaccac cgactcgcca
     1201 gattcgatca tcttcttgaa cagcacccag atgctgatcg atttcatcaa cagtggagag
     1261 attggcgttg gcgatatcca gcagcacacc tacaagtcgc tcggcaaggg acacggctcg
     1321 gctctgcgtg atatggcgtt gaggccggat ggcagcgccg tcctcatctc ggacgcgggc
     1381 ggcaatatcc tggaatatca cacggatacc caaaaaatca ctccctttgc gcagaacctc
     1441 ggcggtgtgc agggattggc cttcgactca aatggcaacc tcttcgcggc agtcggcggc
     1501 caggtgatcc agcttgatcc ggccaccggc aagcagctca agacctacac gctaccgggg
     1561 ggcagcgatg gcatggccta cgatggacgc cataaagtcc tcgacatcgc tgttggcaac
     1621 acgatcatct cgcttgatcc tgccacgagt aaaacagcga cagtcattga cggcatttcg
     1681 atccctgacg gcctggctat cgaccggcaa ggcaatctct tcatagccga cgacatcggc
     1741 gtgttggagc ttaacacaaa caaccagttg ctgatcgtcg gcacgaacac gaatggcatc
     1801 acatgggacg atgtcgcgcc gctctccggc aacggcgccg cgagctatta gctgaattca
     1861 tagcgccaga tggggatgtc ccgccattcc cacctggcgc gcacaagctc ctccagggag
     1921 gtatgttcgc accagtattc gagcgcgtac ctggtcgcgc ggtggccgat caccacgatt
     1981 gacttgccgt catatgtttg aaggacatcg cgcaagaatg cctcgacccg attcgcgacc
     2041 atcaccatgc tctcgccgcc tggaaatggc tcggtgacga ggcgcgcgaa ctcctgctca
     2101 acctgcgcca ccggatactg tgtcattgtg ccataatcgt actcgcgcaa gcgggcatcc
     2161 ggcacaatgg gcagcccgcg cgccgagaac gcaatctcgg cagtcgtcgc ggcgcgctgt
     2221 aaatcggagc aaaagactgc ctccactgct aacgtttcgt aatgtttccc cagttcacgc
     2281 gcccgctgcc gaccgagttc cgaaagcggc acgtcggcat ggccggaagc gcgccccgcc
     2341 tcgttatcga tactggttgc atgtggcgaa tagaagaccg tgagcatcac ttctaccttt
     2401 aagacctcaa gaatttgcta gtgcaatttc tcaaaaatta tcgtatacta tttaaatgga
     2461 atagacgatt gtccctacgt tctaatcaaa tctcgtacag caatcacaca catagcaacg
     2521 ttgtatcaga gtctggggga taccatgcta cgctgcgaat tttgtggtgc cgaattacct
     2581 ccgaattacc atttttgcgg caattgcggc catgtgctga acgatccatc gtttccaacg
     2641 gtgatcactg cgcagactgt atcacaagat gccactttga ggcaacctgt ctccgatgtt
     2701 ttatccatac ctgctgactt ctccatctct aatgagcgcc gaacagaaga aaatcaggct
     2761 gttctgcctg aaatacaaat accgtcggat cttctcctac ccttcgcgac gctggcaggc
     2821 caggcgccag caggtcaggc accggtagct cagggaacac cccaaatcgg tgaagttccg
     2881 catgtccagg gaacgccatc ggcgcctcct ggccatgctc cagcacatgc ccctatgcat
     2941 catatcactc cacagatggc ccctcatgcc gagtctgcgg cagaactatc acgagcgacc
     3001 gcgcccatac accatcatgc agaaccaacg cctcagatgc cacaccatca tgcagagcca
     3061 acgcatcaac tgcatatctc acgacatcct accgcgcccc tccatcacca gggaacgcct
     3121 caacaagatc atcctgccca acacccgcgc cacgagcaag agccgggaca cgaacacgag
     3181 caggcgcacc accgcgctga acacaccggg cggccacatc accggttgcg tctgcgcagc
     3241 catggaacgc ataccgcaca cgccgctacc gcctctaaag tggcagtcgg cacggcgaca
     3301 aagtggctga tcgtcgccct gacggcggtg gtcgtcatcg ccaccagtgg agtcgtgctc
     3361 gtgttggcca gcgcccccgg actttccctg agcggcagca gcagttcgat ctctagtggt
     3421 ggcacgctgc atattcatgg caagggattc ttgcccggag gcagcgtgac gctgactctc
     3481 gataatggcc agcacatcgc ctttcagcgc aacgggctgg caaagagcgc tggtcaaggc
     3541 tctgtacagg aagccgatgt cttattcgcg ctggatcgcg cagccgccag cacgaatgct
     3601 tccatccctg tgagcgtgac cggtacattt gacgcggatg tcacggcccc atcatcgtgg
     3661 tcgccaggac agcatatcat ccacgccagc gagggcttcg gttcgcgcag cgcagatttg
     3721 cagtttacac tgctcgcctc ccctgcaaag ctggtcgtca cccccactgc aatagccttt
     3781 accgcccagg cgggcacaaa ggagagccga tcactgcaaa tcagtaacgc agggcaggca
     3841 accctgacct ggaccgccgc aaccgatggc gcaaaatggc tcagcctgca aggaaatggc
     3901 gggcagatcg gcttcaacgg ccaggcagcg gtcatggatg tgaccgccga cgccaaccat
     3961 ctcaaagtag gaacctatcc agccaccatc acggtgagtt ctaatggagg caatcagcag
     4021 gttgcggtcg cgttgcaggt gattcaagcg acgggcaaaa agcaagcgca acttgacgtg
     4081 agtcccagga cgctcgattt cggcctgctc tcgcccggtc aacaactcac ccagccgctc
     4141 accgtcggca acctcggcac ggctgctctg ctctggcacg ccagcgcggt caatgcgagc
     4201 tggctatcgc tcagcacaag cagcggctcg ctccagcccg gggccatccc acaggcggta
     4261 caggtcactg tcaacacgaa cgggctggca gcgggtagct acgccgctac tatcactgtc
     4321 gcctcaaacg ccggaactgt acagataccg gttgcctttg acgtcctcgg cacaacaccg
     4381 acaccacttc cgccgtcgcc gaccgcgccg cctccgccgt caccaaccac gccgcctccg
     4441 ccatcgccga ccgcgccgcc tccgccgtca ccaaccacgc cacctccgcc atcgccgacc
     4501 gcgccacctc caccgtcgcc aacggtgcct ccatctccga ctgcgccgcc acctaccacg
     4561 tggtctgtaa gcccgacttc gctagatctg accaactgta ccagcggaag tggaccaacc
     4621 tatatgtgtt tcgttaccct gacagagggt tccagttctc ctggcaatat caactggagc
     4681 gtgagtgaca gtctgggaac cgcgggcttc tcccaatcca gcggaacgct cacgccgggt
     4741 ggatcggtgc aaattaccat ct
//
LOCUS       DASZAK010000616         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_334895, whole genome shotgun sequence.
ACCESSION   DASZAK010000616 DASZAK010000000
VERSION     DASZAK010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334895"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VGT44_10795"
     CDS             complement(<1..253)
                     /locus_tag="VGT44_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581327.1"
                     /translation="MYNLLSDARLYRRLIAMQLRSQLQYKTSMLIDIGTYFLVTGIEF
                     LTMLLYFIPFPTMLGWHVGEVALLTAITSFSFGLAELFGS"
     gene            complement(276..1070)
                     /locus_tag="VGT44_10800"
     CDS             complement(276..1070)
                     /locus_tag="VGT44_10800"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV2581328.1"
                     /translation="MLRFYYEVARSAFRRQLIYRWANIAGLLTNIVFGIIFSYVMLAL
                     YQVRSSAGGYNLNDALRYVWLVQSMVMTVLTFGWYELMLTIRGGEVVSDLSKPCDFYW
                     YWFSREIGRSCYYLLYRGLPTYLAGMLLFRFGAPLIWASWPMFLLSLAIGTMTGIAYR
                     VLFNLAAFWIIEARAVGTLATVFALVFTGNYVPLPFFPGWLRAIADWLPFSGLMSVPA
                     QIFLGKLVGLDLLLVSLRQLGWLIVLTLVVRYVAAQATRRVVIQGG"
     gene            1291..2331
                     /locus_tag="VGT44_10805"
     CDS             1291..2331
                     /locus_tag="VGT44_10805"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2581329.1"
                     /translation="MEAALVGASGRTTLGTATLSIGRRADNILVLEDAKSSSHHAEIR
                     PMGEGYCIVDLGSTNGTFVNEQRLGSNTPQTLNANDIIRIGDTRFTYEAQTYIPTVFD
                     APQNNQAYLPTVAAAPPAFPGAPQNPAPGFYSSSPAYPPAEQIPPYGATVAAPSPYTA
                     YQGGQGQVPPPPAYDIPSPYQQPPQYYPPYGGQPPAPAAPPNQPSGASLTTRFKTNQR
                     FRLYVIGAGAVILLLIIGFVVYAAGSTPTKTLNQYCNDFKAGDFSSAYDLLSSTLQTN
                     ISRAQYISIEQKLFTPVGGVTTCSVSNVNESDPSANGNIDYTLGNGQAATGVYTLVDQ
                     NGGWKVSNEVFR"
     gene            complement(2333..>3672)
                     /locus_tag="VGT44_10810"
     CDS             complement(2333..>3672)
                     /locus_tag="VGT44_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2581330.1"
                     /translation="ALARVLERYGSEPLQRRFLPGLIALTTGAVQQGAMFLTEKQGGS
                     DVGQTATIAKRRVFDEDDHEFQPEWELWGDKWFCSNVSADIILTLARPEGAPAGTRGL
                     GLFLVPRTLEDGSRNTYSINRLKDKLGTRSMATGEVTLHGALAYQVGALDRGFVQMTE
                     MINLTRIWAAIGSLAAMRRSFLEALVHTRGRVAFGRPLADHALMRRQLVDLLVEIEGC
                     AALVFETAAVLERVDRHGAEEDRRLLRILTPLCKYYVPKRGEYVALEALEMRGGNGYV
                     EDWPNARLLRDCIVNVIWEGSSNVIVLDVARAMQREGAGVALSKMLHHRLQGLQHPLI
                     APAAAQAQFFARDLVARVQQLNDLDFDHAQLPLRGLVERLTQLTIYTLLLEQAEAELS
                     RGHAGTGQRKWLVAQLFLRRHIQPHPDGWAADDDATAFKHFDALVDWKPVAVD"
BASE COUNT          836 a         1121 c         1044 g          671 t
ORIGIN      
        1 cggagccaaa gagttcggcc aatccaaagc tgaacgaggt gatggcggtg agcagagcaa
       61 cctccccgac gtgccagccg agcatcgtcg gaaaggggat aaagtagagc agcatcgtca
      121 ggaattcaat accggtcacg agaaagtagg tgccgatatc gatcaacatg ctggttttgt
      181 actggagctg agagcgaagc tgcatggcga tcaggcggcg atagagccgc gcgtcgctca
      241 agagattgta cacgatgcat ttccctttct cacagttagc cgccctggat cactacgcgg
      301 cgcgtagcct gagccgcgac gtaacgcacg acgagcgtca aaacgatcaa ccatcccaac
      361 tggcgcaggg ataccagcag caggtcaagg ccgaccagct tgcccaggaa gatttgcgcc
      421 ggaacgctca tcaggccgct gaagggcagc cagtcggcaa tcgcgcgcag ccatccgggg
      481 aaaaatggca gcggcacgta gttgccggtg aagaccagcg cgaacacggt agcaagcgtt
      541 cctaccgcgc gagcctcgat gatccagaag gcagccaggt tgaacaagac gcgataggcg
      601 atgccggtca tcgtgccgat ggcgaggctg agcaggaaca tcggccagga tgcccagatc
      661 agtggcgcgc cgaaacggaa cagcagcatc ccggccaggt aggttggcag gccgcgatag
      721 agcaggtagt agcagcttcg cccgatttcg cggctgaacc agtaccagta gaagtcgcag
      781 ggcttgctca ggtctgaaac gacttcgccg ccacgaatgg tcagcatgag ttcgtaccac
      841 ccaaaagtca gaacggtcat gaccatcgat tgcacgagcc agacatagcg cagggcgtcg
      901 ttcagattgt agccaccggc gctgctgcgc acctggtaga gggcgagcat gacgtagcta
      961 aagatgatcc cgaaaacgat gttcgtgagc agcccggcaa tgttggccca gcgatagatc
     1021 agttgtcgcc ggaaggccga gcgagccact tcatagtaga aacgcagcat cacttcctct
     1081 cctgtatttt tccagcacaa aaacccagca acccacccga caggggggtt ctggtttctc
     1141 gtctttgcgc gccgcacaca cagaaatgcg ggatagccgg aagaccaccc cgtgtctgct
     1201 ttgcattaca aagacattgc acagtctacc acgtcatggt aaaattggtc aacagttttg
     1261 tgcaagttat ttttgacagg agcaaccgcc gtggaagcag cgctcgtagg tgcatcagga
     1321 cgcacgaccc tcggaaccgc tacactctct atcggtcgaa gggcagataa tatactcgta
     1381 ctggaggatg caaagtcatc ttcgcatcac gcggagatca ggccaatggg ggaaggatat
     1441 tgtatcgttg acctgggcag cactaatggc acatttgtca acgaacaacg acttgggtca
     1501 aatacccccc agacactcaa tgccaacgat atcatccgta tcggcgatac gcgctttacc
     1561 tatgaagctc agacgtatat tccaacggtc ttcgatgcgc ctcagaataa ccaggcgtac
     1621 ttgccgacag tagcggcagc accgcccgct tttcccggcg cgccacaaaa tcccgcgcca
     1681 ggcttttata gctcttctcc ggcctatcca cccgctgagc aaattccgcc atatggggca
     1741 acggtagccg cgccatcgcc ctacaccgcc tatcagggcg gccagggtca ggtgccgcca
     1801 ccaccggctt acgatatccc atcgccctat caacagccgc ctcaatacta cccaccgtat
     1861 gggggacagc cgcccgcgcc cgctgcgccg ccgaaccagc catccggcgc atcgctcacc
     1921 accaggttca agaccaatca acgcttcaga ctctatgtca tcggcgctgg cgcggtgata
     1981 ctcctgctca tcatcggctt tgtcgtctac gcagcaggtt caactcccac aaagacactg
     2041 aaccagtatt gcaatgattt caaagcggga gattttagct cggcctatga tctgctgtca
     2101 tcaaccctgc aaacaaatat ttcaagagcg caatatattt cgatcgaaca aaaacttttc
     2161 accccggtcg gcggtgtgac aacctgctcc gtatcgaatg tcaacgaaag cgacccgtca
     2221 gccaacggta atatcgacta tacccttgga aatggtcaag cggctacagg tgtctacaca
     2281 ctggtcgatc aaaatggcgg atggaaggtc agcaacgaag ttttccgata ggtcaatcga
     2341 ccgctacagg cttccaatcc acgagcgcgt cgaaatgctt gaaggcggtc gcatcatcgt
     2401 cggcggccca gccgtcgggg tgcggctgga tgtggcggcg caggaagagt tgggcgacca
     2461 gccacttgcg ctgtccggtt ccagcatggc cacgcgatag ttccgcctcg gcctgctcga
     2521 gcagcagcgt gtagatggta agctgcgtca gccgctcaac cagtccacgc aggggtagct
     2581 gagcatggtc gaaatcaaga tcattcaact gctgcacacg cgccacgaga tcgcgcgcga
     2641 agaactgcgc ctgcgccgct gccggggcga ttagcgggtg ttgcagccct tgaaggcgat
     2701 gatgtaacat ctttgaaagc gcaactcccg ctccctcgcg ctgcatggcg cgagcaacgt
     2761 ccagcacaat cacgttgctg gaaccctccc agatcacatt cacgatgcag tcacgcagca
     2821 ggcgcgcatt cggccaatcc tcgacgtagc cgttgccgcc gcgcatttcc aacgcttcta
     2881 gcgcgacgta ctcgccgcgc ttggggacgt aatatttgca gagcggcgtc aggatgcgca
     2941 ataaacgacg atcttcttcc gcgccgtgtc gatcgacgcg ctccaggacg gcggcggttt
     3001 caaagaccag ggcggcgcaa ccttcgattt cgaccagcaa atcgacaagc tggcggcgca
     3061 tcagtgcatg gtccgccagg ggacgcccga aagcgacccg cccgcgtgta tgtacaagcg
     3121 cttcgaggaa gctgcggcgc atggcagcca gcgagccgat ggcggcccag atgcgcgtca
     3181 ggttaatcat ctcggtcatc tgcacgaagc cgcgatccag cgcgcccacc tggtaggcca
     3241 gcgcgccatg cagcgttacc tcgcctgtcg ccatcgagcg cgtccccagc ttgtccttga
     3301 gccggttgat gctgtaggtg ttgcggctgc catcctccag cgttctggga acgaggaaga
     3361 gacccagccc gcgcgtcccg gctggcgcgc cctctggccg ggcgagcgtg aggatgatat
     3421 cggcggagac gttggagcag aaccatttgt cgccccacag ttcccactcg ggctggaact
     3481 catggtcatc ctcatcgaat acgcgccgtt tcgcaatcgt ggcggtctgg ccaacatccg
     3541 acccgccctg cttctccgtc aggaacatgg ccccttgctg cactgcgccg gtggtcagcg
     3601 cgatcaggcc cggcaggaag cggcgctgca acggctcgct gccgtagcgt tccaatacgc
     3661 gggcgagcgc at
//
LOCUS       DASZAK010000617         6151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_690987, whole genome shotgun sequence.
ACCESSION   DASZAK010000617 DASZAK010000000
VERSION     DASZAK010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690987"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VGT44_10815"
     CDS             complement(<1..439)
                     /locus_tag="VGT44_10815"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2581331.1"
                     /translation="MVEHAEHALADHHTPLLEIQGISFGYGRQALLYEVSLTVRSGEM
                     VGLLGPNGSGKTTLLRLIGGILQPQQGAIKLDGRDVRSWGRREIARRVAVVPQELPVP
                     FAFSVEQLVRLGRTPYLNLLGNLTRQDLAIVQDAMRAADIEQQA"
     gene            complement(433..1500)
                     /locus_tag="VGT44_10820"
     CDS             complement(433..1500)
                     /locus_tag="VGT44_10820"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV2581332.1"
                     /translation="MKSIRVTIQGLPAPSILAALGVALLAAIFLGVSFGTIAIPVSTV
                     ARIVLNATGLFHFARQWDASDELIILQLRLPVVIGAGLVGMALAVAGVLFQGMLRNPL
                     ADPYLIGTSSGAALGAAIAFILPYSTIYGSYFPLTPVLAFAGAMLTVLFVYAIARVGG
                     QTPVVTLLLAGVVINAVLVAIQTLLLTLSPRIQFGLQGLFSWLSGGIAVTTWAPVLVV
                     GAIILGGFAGALLFAGVLDVFGLGEESAEYLGLRVERYKLLIIVVGALLTAAAVSISG
                     LIGFVGLVVPHTMRLLFGPRHRLLLPASALAGAIFLILADLLARVVIAPAVLPVGVFT
                     ALVGAPFFLFLLRNSKRTYRW"
     gene            complement(1542..2552)
                     /locus_tag="VGT44_10825"
     CDS             complement(1542..2552)
                     /locus_tag="VGT44_10825"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2581333.1"
                     /translation="MRSYVISSRFLFSLVALFVLLLSGCGQIAGGSASTPTPGATPTA
                     ALPVLTQDAYGKPIVFPSTAPQHIVSLVPNISEILGALHLEGRVVGVDYYTNYPTNLT
                     SLPKVSDANGKYNVEQIAALQPDLVLSYGQDTKQYDAQLQSVGENVVDLPAGNLTLTL
                     QEILTVGRLTFTSATATTLVNQLQQQINQVKTAVAGATAPKVMIELDDSTPGKPYVFG
                     GGSFGDELIQDANGVDIFHDNTTNGGFPQVTDEAVISANPQFIILTEDPAYGGDVNAV
                     YKRPNWGGIDALKLRQVYRINPNLIGRAGPRLVEGLQCLAQIIHPAQFSGSLPAYCSG
                     TV"
     regulatory      complement(2682..2894)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3017..3649
                     /locus_tag="VGT44_10830"
     CDS             3017..3649
                     /locus_tag="VGT44_10830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV2581334.1"
                     /translation="MSNERDFFRQVMGQFPTGVTVVTTRSSEGLSGITVNSFTSVSLE
                     PALVLICIDLKSHTLPFLRASGSFAVNFLTSEQEDLSRCFAKTAAERNAYFCNATYHE
                     AATGSPILDGTLAFIDARVVAEYPGGDHAIFLGKVEAMGTHGRVMYLNDEGKAHSSLA
                     AGNDGVSSNGHAPSTGEEPTPLAYHRGKYRHLATDYLAPSLVASYEKEHR"
     gene            3721..4371
                     /locus_tag="VGT44_10835"
     CDS             3721..4371
                     /locus_tag="VGT44_10835"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV2581335.1"
                     /translation="MPYDTSSPAEYGDRITAIAALQQRILACRLCEQQGYINAARPLT
                     SGRASDRIMVIGQAPGHRSVANGRSFSGPGGGVLQRWLEQAGFPPGYLHTHAYLSSLT
                     HCDPGPNPRGNGDRRPSPQELALCRPFLDVELRLLQPKVVLLVGAMAIEAFLGKVKLE
                     DVIGTYTERDGMLLLPLPHPSGVSRWLLDAAHQALLHKALAILAQWRVTYALSEEA"
     gene            complement(4347..4766)
                     /locus_tag="VGT44_10840"
     CDS             complement(4347..4766)
                     /locus_tag="VGT44_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEV2581336.1"
                     /translation="MDRQEAIEFLLDHYENPRNYGPLEDADVSLSGGNPGCADIITMH
                     ARFDDKGKIESIHWEGEGCTISRAAASYVTELTQGMTAEEVENLSFEDLMEQLGRELV
                     MTRPTCATLALGTLKQGVHEYHMHQLAGNDHASSDKA"
     gene            complement(5032..5916)
                     /locus_tag="VGT44_10845"
     CDS             complement(5032..5916)
                     /locus_tag="VGT44_10845"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV2581337.1"
                     /translation="MSGTETATSTTQAGTGYAHPEVLVETSWVAEHLNDPSVRLIEAD
                     EDVLLYEVGHLPGAVKLDWHVDVQDPLDRDFIDQEGFEKLMSHWGVGKDTTIVLYGDR
                     NNWYACYSFWLFNMFGHSNMKIMNGGRAKWEAEGRELTRDIPHFASTSYHAQARDESN
                     RAYRDDVMSGLKEANRRLIDVRSPQEYTGELLHMVNYPQEGAQRGGHIPTAKSIPWAT
                     AANADGTFKSVDELRQIYGGKDIQPGNDVVTYCRIGERSAHTWFVLTQLLGYPQVRNY
                     DGSWTEWGNLVRAPIEKP"
BASE COUNT         1280 a         1863 c         1801 g         1207 t
ORIGIN      
        1 cggcctgctg ctcgatatca gcggcgcgca tggcatcctg cacgatagcc aggtcctggc
       61 gggtcaggtt gccgagcagg ttgagatagg gcgtgcggcc taatcgcacc agttgttcca
      121 cactaaaggc gaagggaaca ggcaattcct gggggacgac ggcgacacga cgggcgatct
      181 cgcgcctgcc ccacgagcgc acgtctcgcc catccagctt gatcgcgccc tgctggggct
      241 gcaagatacc gccgatgagg cgcagcagcg tcgttttgcc ggaaccattc ggtcccagca
      301 agcctaccat ttcgcccgaa cgcacggtaa gcgacacctc atagagcagc gcctggcgtc
      361 catagccgaa gctgatgccc tgtatttcca gcagcggtgt gtggtggtct gcgagcgcat
      421 gctccgcgtg ttctaccatc gataggtccg tttgctgttt ctgagcagga acaaaaagaa
      481 gggcgcaccc accagggcag tgaagacgcc aacgggcagc acggcgggcg cgatgacgac
      541 gcgggcgagc aggtcggcca ggatcagaaa gatggccccg gccagcgccg aggcgggcag
      601 cagcaggcgg tgacgcggcc cgaatagcag gcgcatggta tgcggcacca ccaggccgac
      661 gaagccgatc aacccgctga tcgagacagc cgccgccgtt aagagcgcgc caacgacgat
      721 gatgagcagc ttatagcgtt ccacgcgcag acccaggtac tcagcgctct cctcccccaa
      781 tccaaagaca tccaggacgc ccgcgaacag cagcgcaccg gcgaagcctc ccaggataat
      841 cgcgccgacg acgagaacgg gcgcccaggt tgtgacggcg atgccgcccg acagccagct
      901 aaacaggccc tgcaagccga actggatacg cggactcagc gtgagcagca gcgtctggat
      961 agcgaccagc acagcgttga tgaccacccc ggccagcagc agtgtcacga cgggcgtctg
     1021 gccgcccacg cgcgcgatgg cgtagacgaa gagtacggtg agcatcgcgc ccgcgaaggc
     1081 cagcacggga gtcaggggga aatacgagcc atagatcgtg ctgtagggca ggatgaaggc
     1141 gatggccgcg cccagagccg cgccggacga cgtgccgatc aggtacgggt cggccagggg
     1201 attgcgcagc atcccctgga acagcacccc cgcgacggcg agcgccatcc ctaccagtcc
     1261 cgcgccgatc acgaccggca gccgcagctg caggatgatt aactcatcgc tagcatccca
     1321 ttgccgcgca aagtgaaaca gcccggtggc gttcagcacg atgcgcgcaa cggtcgaaac
     1381 aggaatcgcg atcgtgccga agctgacgcc cagaaagatt gccgcaagca gcgctacgcc
     1441 cagcgccgcc agaatcgacg gcgcgggcag gccctggatg gttacgcgaa tgcttttcaa
     1501 cggtactgat gctttcctat cccgcgcaca ctgtacatcg attataccgt tccactgcaa
     1561 tacgcgggca gcgaaccgct gaattgcgcc gggtgaatga tttgcgccag gcattgcagg
     1621 ccctccacca ggcgcggacc ggcgcgaccg atcaggttag gattgatgcg atagacctgg
     1681 cgcagtttca gcgcgtcgat gccgccccag ttgggacgct tgtagacagc gttgacatcg
     1741 ccgccataag ccgggtcctc ggtcaggatg atgaactgcg ggttggcgct gatgaccgct
     1801 tcatccgtga cctgggggaa accgccattg gtggtattgt cgtgaaaaat gtccaccccg
     1861 tttgcatcct ggatcagctc atcgccgaac gagccgccgc caaagacgta gggtttgccc
     1921 ggcgtgctgt cgtcaagttc aatcatcacc ttcggggcgg tcgcgccggc taccgccgtt
     1981 ttgacctggt tgatctgctg ttgcaactga ttgacaagcg tcgtcgcggt cgccgacgta
     2041 aaggtcaagc ggcctaccgt caggatttcc tggagcgtca acgtcagatt tccggcgggc
     2101 aggtcgacga cattttcccc gacgctctgc aattgagcgt cgtattgttt ggtatcctgg
     2161 ccatagctca gcaccaggtc gggctggagc gcagcaattt gctcgacgtt gtacttgccg
     2221 ttggcgtccg agaccttcgg cagcgaagtc agattggtgg gatagttcgt gtagtaatca
     2281 acgccgacca cgcgcccttc caggtgcagc gcgcccagta tttcgctgat gttgggaacc
     2341 agcgacacga tgtgctgggg cgcggttgag ggaaagacaa tcggcttgcc gtaggcatcc
     2401 tgggtcagga cgggcagcgc ggcggtcggg gtcgcaccag gcgtgggcgt tgacgcgctt
     2461 ccaccggcga tttgaccgca gcccgacagg agcagcacaa acagagctac gagggagaac
     2521 aggaaacgag atgagatgac ataggagcgc attttgcaaa acctccgaga cataccaacg
     2581 ctcgctgtca ccccgcgtgg gtgtggagat caaccagggc aaaatgcagg tagcgtgaat
     2641 acgattgtcc cggcggctcg ctctcctcat tacgcgaagg atcatgagca acatcaccgg
     2701 gcaggtctcc tggctcctgg ctacaatcgc caatcacacg gccttcccat ccctcgcggg
     2761 gacagtggtg aaacgctcga tgcgagcggg tgattggctc accagttaca gtagcgcgac
     2821 tgcgtcggat tctcaccgac ttccctattc tgcctgagta tccaacccag gccacccggc
     2881 gtagatagaa acgcagcttc attatactga acttctgtac agttgacaag cgcagctaag
     2941 aggatgatac tagtggtatc acatagcggt gtagcatggc gaccgcaata cttttcatat
     3001 aaaggattcc tggtacatgt ccaatgagcg agattttttc cggcaggtga tggggcaatt
     3061 cccaacgggc gtcaccgtcg tcacgacacg cagcagtgaa ggactctccg gtattacggt
     3121 caattccttc acgtccgtat cgcttgaacc ggcgcttgtc ttaatctgca ttgatctgaa
     3181 aagccatacc ctgccattcc tacgcgcaag cggctcgttc gcggtgaatt tcttgacgag
     3241 cgagcaggag gacctgtcgc gctgcttcgc gaaaactgct gccgagcgca atgcctactt
     3301 ttgcaacgcc acctaccatg aggccgcgac cggctcgccc atcctggatg gcaccctggc
     3361 ctttatcgat gcccgcgttg tcgcggaata ccccggcggc gaccacgcca tcttcctcgg
     3421 caaagtggaa gcgatgggaa cgcatggccg ggtcatgtac ctgaatgacg agggcaaggc
     3481 acacagctcg cttgctgctg gaaatgatgg cgtatcaagc aacggacatg ctccaagtac
     3541 cggtgaggag ccaacgccgc ttgcatatca ccggggtaaa tatcgccacc ttgccacaga
     3601 ttatctcgct cccagtctgg ttgcaagcta tgagaaagaa caccgctaac gcgccaaacc
     3661 ctcgcatttt cggcggaagc aaacagttca tcgaaaattt aaccgctcac aaagatcaag
     3721 atgccctacg acacttcttc acctgctgaa tatggagacc gaatcaccgc tatcgccgcc
     3781 ttgcagcagc gcatcctcgc ctgccgcctc tgcgaacagc agggctacat caatgccgct
     3841 cgtccgctca caagtggccg ggccagcgat cgcatcatgg tcatcgggca ggcgccggga
     3901 catcgctccg tggcaaatgg acgttcgttc agcgggcctg gcggcggcgt attgcaacgc
     3961 tggttggaac aggcggggtt tcctcctggc tacctgcaca cccacgccta tcttagctcg
     4021 ctgacgcact gcgatcccgg acctaacccg cgcggcaacg gcgaccggcg gccttcgccc
     4081 caggaacttg cgctgtgcag gccatttctc gacgtggaac tgcgcttgct gcaaccgaaa
     4141 gttgtcttgt tggttggggc gatggcaatt gaggcgttcc tgggcaaggt gaaactggag
     4201 gatgtgatcg ggacatacac cgaacgtgac gggatgctgc tcctgccatt gccgcatcca
     4261 tcgggggtca gtcgctggct gctcgatgcg gcgcaccagg cgctgctgca caaagcgctg
     4321 gcgatactgg cgcagtggcg cgtgacttac gccttatcgg aagaggcgtg atcgtttccg
     4381 gccagttgat gcatatgata ttcatgcacg ccctgcttga gtgtgccgag cgccagggtt
     4441 gcgcaggtgg ggcgcgtcat gaccagctcg cggccaagtt gctccatcag gtcttcaaag
     4501 ctcagatttt ccacttcttc cgccgtcatg ccctgggtta attcggtgac atacgaggcg
     4561 gcggcgcggc tgatcgtgca gccctcgccc tcccagtgaa tcgactcgat tttgcccttg
     4621 tcgtcgaaac gcgcgtgcat ggtaataatg tccgcgcagc cgggattgcc ccctgacagg
     4681 ctcacatcgg catcttccaa cggcccgtag ttgcgcgggt tttcgtaatg atccagtaaa
     4741 aattcaatcg cttcctgacg atccatattg atttcctttg tatagttgag gacgagggcg
     4801 accgcaaggg tctccacccc acatccaccc tacccccgcc cttacaatga ctacgatatt
     4861 acctcatccc gtatctttgt aagggcgggg gtagggtgga tgtggggtgg agacccttgc
     4921 ggtcgcccta tcgtttcata tcgcgccatc ctcgccagcc tcaaacgctc actgggcggt
     4981 ggagaccctt gcggtcgccc tgtcgtttca tatcgagacc agcctcactt cttacggttt
     5041 ttcgatgggc gcgcgcacca ggttgcccca ttcggtccag gagccgtcgt agttgcgcac
     5101 ctgagggtag ccgagcaact gcgttagcac gaaccaggtg tgcgcggaac gctcgccgat
     5161 gcggcagtag gtcacgacat cgttgccggg ctggatatct ttgccgccgt aaatctggcg
     5221 caattcatcc accgacttga acgtaccatc ggcgttggcc gctgtcgccc aggggatgct
     5281 cttggcggtc gggatatggc cgccgcgctg cgcgccctcc tggggatagt tgaccatgtg
     5341 cagcagctcg ccggtgtact cctgtggaga gcgaacatcg atcaggcggc gattggcttc
     5401 cttcagcccg ctcatgacat cgtcacgata ggcgcggttc gactcgtctc tggcctgcgc
     5461 atggtagctg gtcgaggcga aatgggggat gtctctggtc aactcgcgac cttcggcttc
     5521 ccattttgcg cgcccgccgt tcatgatctt catattgcta tgcccaaaca tgttaaagag
     5581 ccaaaaagag taacacgcgt accagttatt ccgatcccca tacagcacga tggtcgtatc
     5641 cttgccaacg ccccagtggc tcatgagttt ctcgaaaccc tcctggtcga tgaagtcgcg
     5701 atccagcggg tcctgcacat cgacatgcca gtcgagcttg accgcgccgg gcaaatgccc
     5761 aacttcgtag agcaacacat cctcatccgc ttcgatcagg cgaaccgagg gatcgttcag
     5821 gtgttccgcg acccagctcg tctccaccag cacttctgga tgcgcatagc ctgtccccgc
     5881 ctgcgtcgtt gatgttgcgg tttccgtacc agacatacta aaatcctccc agttaaacac
     5941 attgttttgc caaacctgcg tagagtgcag attcgagata acacgtcatt ccgttatgac
     6001 aacaaaagtg acctgtccta tattctacta gccaactcgg aattaagcaa gaggaaattt
     6061 tgcaaaatgt catgctgagc gcagcgaagc atctcggacc atcggagtag atgcttcgct
     6121 acgctcagca tgacagaagg aggtgccttc g
//
LOCUS       DASZAK010000618         5296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_87154, whole genome shotgun sequence.
ACCESSION   DASZAK010000618 DASZAK010000000
VERSION     DASZAK010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87154"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1435)
                     /locus_tag="VGT44_10850"
     CDS             complement(143..1435)
                     /locus_tag="VGT44_10850"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV2581338.1"
                     /translation="MTVDISDIESIIARQNTWRQTQTINLIASENTPSEAVRRVQLSD
                     FMGRYAEGHPNEGDKVNRYYQGTRFIDEIERMATKEIIELFGAKQADVRPYSGNNANT
                     AIAMGWLRGGDTVVANSTDAGGHISHGPVGVFGRRIQNRGQSLKLGGPKSINLHILPL
                     TEDHYHVDAQKTIELIDQVSPQMVIMGKSLFLFPEPVEEVAAYCKTRDIPLLYDGAHV
                     LGLIAGGEFQQPLHEGATWMTASTHKTFPGPQRGVILGNLDEEGAAKFWPAADRGVFP
                     GTSSNHHLYSLPALVVATREMQRYGRAYAAQIVRNAQALGRSLDELGTPVEARDFGYT
                     RSHMIAVNVAQWGGGVEVAKRLEASDIIVNYNMVPGDTDPRNPSGLRIGVPEMTRFGM
                     DERAMGELAQLIHDAVRAKDVKAGVHALRGRFVEMRYV"
     gene            1609..3441
                     /locus_tag="VGT44_10855"
     CDS             1609..3441
                     /locus_tag="VGT44_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2581339.1"
                     /translation="MGDFNSPGNQLQGRTLGTCTLQELLGRGGMGAVYLAQQSRPRRT
                     VAVKVLLPELFSNPTGFSEFLARFRREADAIAALDHINIMPIYEYGEQDRLAFLVMPY
                     VSGGTLRDVVTKRGKLPLREATTIVEQAAAALDYAHQHGIIHRDLKPGNILFHADGRL
                     LLTDFGIAKVLNEPAGPNVVAMQTLTSTGMLIGTPEYLSPEQATGAPLDNRSDIYSLG
                     IVLFQLLSGRVPFSGTTPVAVAIKHATEEPPSLSQLNPEVSPGVEQVIMKALAKKPEQ
                     RYATAGGFARALRAEAGGDRVSEAPTFMTDPRPDATVSTLSSAATIVDATPPVQGKQH
                     SEPTVAEVRHSTKRRSRLPLTFALVSGLLVAVLILGGLAFYLHWLPIGNQQATGSNST
                     PTSVTKTSVPVRTSPTTAVAPPQTALPSPSVPVGKLLYGASLPVCGAQNSLWSTNPAV
                     QVTCDPTDTRLTNTNAGQVEGVLLNNLTGNAPIPNDYVIEVQVKPASSGTFGILFREQ
                     TGAGHKGGYSFIIQPSGSWNGNSIDDSTGQASSLFGRQGTAFSSTGFTTIDVVIQGDT
                     FRLYFNGASQGGITSTTYSSGDFGLAVDGGADVMFKNLAIYALP"
     gene            complement(3467..3799)
                     /locus_tag="VGT44_10860"
     CDS             complement(3467..3799)
                     /locus_tag="VGT44_10860"
                     /inference="COORDINATES: protein motif:HMM:NF007495.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HEV2581340.1"
                     /translation="MITYGEFENVDIRVGRIVKVEDFPQARKPAYKLDIDFGPEIGQK
                     RSSAQITVHYTKDELLGRLVVAVVNFSPRQIGPFMSEVLTLGLPDGEGNVVLLTPTKE
                     VPLGGKMF"
     gene            complement(3933..4286)
                     /locus_tag="VGT44_10865"
     CDS             complement(3933..4286)
                     /locus_tag="VGT44_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV2581341.1"
                     /translation="MRIYVGSLPYQTTDQDLVDLFGQVGEVVSATVIMDRGTGRSKGF
                     GFVEMSNDQDAQKAIEQLNGSLLGNRNIVVNEARERQESFGSNRSGGYQNRGGSGGGG
                     RRSSDRNGGYRDRGY"
     gene            complement(4406..5101)
                     /locus_tag="VGT44_10870"
     CDS             complement(4406..5101)
                     /locus_tag="VGT44_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HEV2581342.1"
                     /translation="MLTLPKAITFDCYGTLIDWESGIQRFFAERLAAHNMEDIDPRAL
                     QHRWEEIQFVSIQQRYRPYREVLRDTMRMTLDTTQIAYAEQDIDAFAASMGKWQPFPD
                     TKQAILDLQRYLKVCLITNTDDDIIAESERTIGVKFDEIITAQQAGSYKPSHKGFLLA
                     RERLGLAVSDIWHAGFGFKYDIVPATELGYTTVWVNRQGEARPVDVKETFLVGDMRTL
                     ALLVKGIAEKNAE"
BASE COUNT         1164 a         1488 c         1376 g         1268 t
ORIGIN      
        1 ttcttcttca ctaatgcctc tctgcgacat cctttcaacc gcatgacgtt ggtagataag
       61 ttcgaattgt tcctccatat caggactcta ctttcagaac aacgtcgaac actgtacaat
      121 tacccacgag taccgtacca aattacacat acctcatctc cacgaatctg ccgcgcagcg
      181 cgtgaactcc cgccttcaca tccttcgcac gcaccgcgtc gtggatcagc tgcgcaagtt
      241 cgcccatggc gcgctcgtcc atgccgaagc gcgtcatttc gggaacgccg atacgcaggc
      301 cggaggggtt gcgcgggtcg gtatcgccgg gaaccatgtt gtagttgacg atgatgtcgc
      361 tggcctctaa gcgtttggcg acttcgacac caccgcccca ctgcgcgacg ttgacggcga
      421 tcatatggct cctggtatag ccgaaatcgc gcgcctcgac gggcgtgccg agttcatcga
      481 ggctgcggcc cagcgcctgg gcgttgcgca cgatctgcgc ggcgtaggcg cggccgtagc
      541 gctgcatctc gcgcgtggcg acgacgaggg caggcaagct gtacaggtga tggttgctgg
      601 atgtgccggg gaagacgccg cgatctgccg caggccagaa tttcgccgcg ccctcttcat
      661 ccaggttgcc caggatcacg ccgcgctgag ggccggggaa ggttttgtgc gtgctggccg
      721 tcatccacgt cgcgccctcg tgcagcggct gctggaactc gccgcccgcg atcaggccaa
      781 gtacgtgcgc gccatcgtag agcagaggga tatctctggt tttacaataa gcggcgacct
      841 cttcgacggg ttcggggaag aggaagagac tcttgcccat aatgaccatc tgcggcgaaa
      901 cctggtcgat cagttcgatg gttttctgtg catcaacgtg gtagtggtct tccgtcaggg
      961 gcaaaatatg caggttgata gatttggggc cgcccagctt gagcgattgc ccgcgattct
     1021 ggatgcgcct gccaaagacg cccaccggcc cgtgactgat atgcccgccc gcgtcggtcg
     1081 agttcgccac aacggtatcg ccgccgcgca gccagcccat cgcaatcgcg gtattggcat
     1141 tgttgccgct gtaaggccgc acatccgcct gctttgctcc gaacagctcg atgatctcct
     1201 tcgtcgccat gcgctcgatc tcgtcgatga agcgcgttcc ctggtaatag cgattgacct
     1261 tgtcgccctc gttggggtgc ccttcggcgt aacgccccat gaaatcggag agctgcacgc
     1321 ggcgcacggc ctcgctgggt gtattttcgc tggcgatcag gttgatggtc tgcgtctgcc
     1381 gccatgtatt ctgccgggca atgatacttt cgatgtcgct gatatctaca gtcatgtgtt
     1441 tgctccctcg tagcgtatgt tattgcggaa atatgtacac agtatatcat cttcgcaaaa
     1501 gggggccatt ctcgttgaca tttgaccggt ttatagcgtc ttgcttcttg ctaatcaagc
     1561 gagcttgata tagaatcgtg gtagagttta aggtaggagg ctaaaactat gggggacttc
     1621 aacagtcctg gcaatcaatt acaaggaaga acgcttggaa cctgtacact ccaggagctt
     1681 ctcggacgtg gtggtatggg tgcggtctat ctagcgcaac agtcaagacc gcgacgcact
     1741 gttgctgtca aagtgttgct gccagaactc ttctcgaacc cgacaggatt cagcgaattt
     1801 ctggccagat ttcgacgcga agctgatgcg attgccgcac tcgatcacat caacattatg
     1861 cctatttatg agtatggcga gcaggatcga cttgccttcc tggtgatgcc ctacgtctcc
     1921 ggcggcacac tacgcgatgt agtaacgaaa cgcggtaaac tgcctcttcg agaggctaca
     1981 accatcgtcg agcaggccgc cgctgcctta gactacgctc accagcatgg cattatacac
     2041 cgggatctca aacccggcaa tattttattc catgctgacg ggcgactact tttaaccgac
     2101 tttggtatcg cgaaagtgct gaacgagcct gccggcccga atgtcgtggc tatgcagaca
     2161 ctgacttcga caggtatgct gatcggcaca ccagaatatc tttcaccaga gcaggctacc
     2221 ggcgctcctc tcgacaatcg ctcagatatt tactcacttg gtatcgtctt attccagtta
     2281 ctgagcggtc gagttccatt ttctggcaca acaccggtgg cagttgccat caaacacgcg
     2341 accgaagagc caccttcgct ttcacagctc aatcctgagg tttcacccgg tgttgagcag
     2401 gtgattatga aggcgctggc caaaaaaccc gaacaacgct acgctacggc tggtggtttc
     2461 gcgcgagcgc ttcgcgctga ggccggcggg gatcgagttt ccgaagcgcc tacctttatg
     2521 accgatccca gacccgatgc taccgtttct actttatctt ccgccgcaac tatcgttgat
     2581 gcgactcctc cagtacaggg gaaacagcat agtgaaccca ctgttgccga agttcgacac
     2641 tcgacgaaac ggcgcagtcg tctgcccttg acgtttgcat tagtcagcgg acttttagtt
     2701 gctgtcttaa tccttggtgg gttagctttt tatctgcatt ggttgccaat tgggaatcag
     2761 caagcaacag ggtcaaatag tacaccgaca tctgttacaa aaacgtcggt gcctgtgcgc
     2821 acatctccaa ctactgccgt cgcccccccg caaaccgcct tgccctcgcc ttcagtgcca
     2881 gttgggaaac tcctctatgg cgcgtctctc ccagtttgcg gtgcgcagaa cagcttgtgg
     2941 agtacaaatc cagctgtaca ggtcacctgc gatcctacag atactcgctt gaccaacaca
     3001 aatgccgggc aggtggaagg agtgttgttg aacaatctca ccggaaacgc tcccatcccc
     3061 aacgattatg tgatagaagt ccaggtgaaa ccagcctcaa gtggcacatt cggtattctc
     3121 tttcgcgagc aaactggagc cggtcataag ggtggttatt cgtttatcat tcagccgtca
     3181 gggtcctgga atggcaatag tatcgatgat tctacaggac aggcttcttc gctctttgga
     3241 cgacagggga cagcgttcag ctccacaggt tttacgacca ttgatgtcgt cattcagggt
     3301 gatacctttc gactttattt caatggagcg agccagggag gcattaccag caccacttac
     3361 tcgagtggcg attttggatt ggcggtagat ggaggagcag atgtgatgtt caagaatctg
     3421 gcaatttacg ctctaccata aggcattgta ttgaaaaact gagaaattag aacatcttgc
     3481 cccccagcgg cacttccttc gtcggggtaa gtaacacaac gttgccctcg ccatcgggca
     3541 ggccaagtgt caggacctcg gacatgaagg ggccgatttg cctgggcgaa aaattgacga
     3601 cggccaccac cagcctgccg agcaattcat ctttggtata gtggacggta atctgcgccg
     3661 atgagcgttt ctgcccaatt tcggggccaa agtctatgtc cagcttgtag gcgggtttgc
     3721 gcgcctgggg gaaatcctcc actttgacga tcctgcccac gcgaatatca acattctcaa
     3781 attcaccata ggttatcata cggtaaacac ctccgttacg gctattatag caagaaaatg
     3841 cctataacaa aagaaagcca gcgcattgct ggctttcttc tgatggtttg ttcgcatcat
     3901 ctcactattg gagacgatgc gcatattctg gcctagtagc cgcgatcgcg ataaccgcca
     3961 ttgcgatctg atgagcgacg accgccgccg ccgctgcctc cgcgattctg gtacccgcca
     4021 ctacgattgc tgccaaacga ctcctggcgc tcacgagcct cgttcacaac gatgttccgg
     4081 ttgccaagca gcgaaccgtt cagttgctcg atggctttct gtgcatcctg gtcgttgctc
     4141 atttcgacga aaccgaagcc cttactgcgc ccggttccgc gatccatgat aacggtggct
     4201 gatacaactt ctccaacctg cccgaagaga tcaacaaggt cttgatctgt cgtttggtac
     4261 ggcaggctac caacataaat tctcactatg gttagctccc acatcgacgt ctactaacat
     4321 attgccgtcg gtggaaacaa ctccacaaaa acgaaccaac aacatctgac gtcatacaca
     4381 cattataaca cacctgtcac gcactttatt ccgcgttttt ttctgcaatt ccttttacca
     4441 acaacgccaa cgtccgcata tcccccacca gaaacgtctc cttcacatca accggcctgg
     4501 cctcaccctg tcgattgacc cataccgttg tataccccaa ttcggttgct ggcacgatgt
     4561 cgtatttgaa gccaaatccg gcgtgccaga tatccgagac agccaatccc aagcgttctc
     4621 tggctaagag gaagcctttg tgcgaaggct tgtatgaacc cgcctgctgc gccgtgatga
     4681 tctcatcgaa tttcacgccg atggtgcgtt cgctttcggc gatgatgtca tcatcggtgt
     4741 tggtgataag gcagaccttg aggtagcgct gcaaatcgag gatggcctgc ttcgtatcgg
     4801 gaaagggctg ccacttgccc attgaggcgg caaaggcatc gatatcctgc tcagcgtagg
     4861 caatctgcgt agtatcgagg gtcatgcgca tggtatcgcg cagcacctcg cgataggggc
     4921 ggtagcgctg ctggatggag acaaactgga tttcttccca gcgatgttgc agcgcgcggg
     4981 gatcgatgtc ttccatattg tgcgcggcca gtcgttccgc gaaaaatcgc tggatgccgc
     5041 tttcccagtc gatgagcgta ccataacagt cgaaggtgat ggcttttggc aaggtcaaca
     5101 taaaaaattc ctccaacctc ctgatttttc tggtgtacga tccgcttgat actgctatac
     5161 tacaacatac taaccgaaaa agatgggtac actgtctttc tgctggcata gaaactcagg
     5221 aaggataaca gtctcatgaa ggtctatact gcggaacaca ttcgcaatgt agcgttgatt
     5281 tcccatgtgg gcgcag
//
LOCUS       DASZAK010000619         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_265056, whole genome shotgun sequence.
ACCESSION   DASZAK010000619 DASZAK010000000
VERSION     DASZAK010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265056"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VGT44_10875"
     CDS             complement(<1..241)
                     /locus_tag="VGT44_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581343.1"
                     /translation="MATSLNQPVPGNLDDFGYPTRPDARGRLRHSGVRASKRILNMGI
                     FVCLSIAGGLLKMSRAGRRYPALPMQFTPVRRILVI"
     gene            complement(514..1740)
                     /locus_tag="VGT44_10880"
     CDS             complement(514..1740)
                     /locus_tag="VGT44_10880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV2581344.1"
                     /translation="MKRLLRGLILLLVRLYGIPGARAAARRARSAAVPASPRILLIRP
                     DHLGDLVLTTPVLHALRQQLPDAHITMAVGPWSSEIVARHPDLDRVVVLPFPGFSRAP
                     QKPLAPYTLLYNAARQLRRGHYDLAVNLRPDFWWGAALLYLAAIPCRAGYALPPATPF
                     LTHALPFTTPELASVSNLRLLSLDLRALGGKPLLEPWSPSVYPLLFRPTTEEQRQVNE
                     RLQQAGIDETIPIIVIHPGSGAAVKLWRSDGWARIAEQLPARLTFSPPARIILTGSKS
                     EQALLDETAQAMRPEQGQEPLILTSLSIGQLAALLGRAMLVLGVDNGPLHIAVAQGTP
                     TLQIYGPTDPRIFGPWGNPERHRVLASTERCASCPAIPCGRLDFTPEELPAHPCVRNL
                     AELTVMDAIQPLLVND"
     gene            complement(1865..2017)
                     /locus_tag="VGT44_10885"
     CDS             complement(1865..2017)
                     /locus_tag="VGT44_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEV2581345.1"
                     /translation="MSEYYCVKCKEKREMKDAERVTMKNGKPALKGKCAVCGTTINTI
                     LSADKK"
     gene            complement(2019..>2277)
                     /locus_tag="VGT44_10890"
     CDS             complement(2019..>2277)
                     /locus_tag="VGT44_10890"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581346.1"
                     /translation="ATLELHPEVVQARADLLAAGCELVRLSGSGPTLYAPFSDLSHAM
                     QAWDRLQAQAYEVYLSRAVYPDKGNIDVYSSNTYTARKEEP"
BASE COUNT          416 a          681 c          726 g          454 t
ORIGIN      
        1 gaattaccag gatgcgccgc accggcgtga attgcatcgg caacgcagga tagcgcctgc
       61 ccgctctgct cattttaagc aatccgccag caatgctcaa gcacacgaag atgcccatgt
      121 tcagaatgcg tttgctcgcc ctgacgccgc tgtgccgcag tctgccccgc gcatccggtc
      181 gagtcggata gccgaaatcg tccaggtttc ccggcactgg ttgattgagt gacgttgcca
      241 tcgatcctct gcctcaacta atacttgaat tattaacgcg gacatccgta tggaagctca
      301 gggcgaccgc aagggtctcc accccgctcc actccacccc cgcccttaca aagactacga
      361 ggccgctgga cccgcctctc gccgttcttt gtaagggcgg gggtggagtg gagcggggtg
      421 gagacccttg cggtcgccct cgtcctccct gatccagtgg ctgcatgatt ttgtcaatgt
      481 gcataatcgg cggtgtgcgc gatcaatcgg cgcctaatca ttcacaagca gcggctgtat
      541 ggcgtccatc acggttagct ccgccaggtt gcgcacacag ggatgggcgg gcagttcttc
      601 cggtgtgaag tccaggcgac cgcacgggat agccgggcag ctggcgcagc gctccgttga
      661 cgccagcacg cgatggcgtt ccgggttgcc ccaggggccg aaaatgcgcg ggtcggtcgg
      721 cccatagatc tggagggtcg gcgttccctg cgcgactgcg atgtgcagcg ggccgttatc
      781 gacgcccagc accagcatgg cgcgccccag gagcgcggcc agttgcccga tgctgagcga
      841 tgtcagaatc aacggctcct gcccctgctc tggccgcatc gcctgcgcgg tctcgtcgag
      901 cagcgcctgc tcgcttttgc ttccagtcag gataatacgg gctggcggcg agaatgtcaa
      961 gcgcgcgggg agctgttctg ctatgcgcgc ccaaccatca ctgcgccaga gtttgacggc
     1021 ggctccgctc cctgggtgaa tcacgataat ggggattgtt tcgtcgatgc cggcctgttg
     1081 caggcgttcg tttacctggc gttgttcttc cgtagtcgga cgaaaaagga gtggataaac
     1141 tgagggcgac catggttcta gtagtggttt gccaccgagc gcgcgcagat ccaggctgag
     1201 gagtcgcaaa ttcgagacgc tcgccagttc cggcgtggtg aagggcagcg catgcgtgag
     1261 gaagggcgtg gcgggtggca gcgcataacc ggcgcggcat gggatggcgg ccaggtagag
     1321 cagcgccgcg ccccaccaga aatcgggtcg caggttgacg gcgaggtcgt aatggccccg
     1381 ccgcagctgc cgcgccgcgt tatagagcag tgtataaggc gcgaggggtt tttgtggggc
     1441 gcgcgaaaag cccggaaagg gcagaaccac cacgcggtcc aggtcgggat gcctcgccac
     1501 gatttcgctc gaccagggac cgaccgccat tgtaatatgc gcgtcgggga gttgttgccg
     1561 cagcgcatgt agcacgggcg tggtcagtac caggtcgccc aggtgatcgg ggcgaatgag
     1621 cagaatgcgc ggtgatgcag gaacggcggc agagcgcgct ctgcgcgccg ccgctcgtgc
     1681 gcctggtatc ccatacaggc gcaccagtag caagatcagg ccgcgcagca gacgcttcaa
     1741 gtaattgcct cgaaaatcgg aatacagctg cccagggcga ccgcaagggt cgcccctacc
     1801 ctgcgacgca gcgcaatgcc tatccgtcag ggtaggggcg acccttgcgg tcgccctggg
     1861 aaccctactt tttatcggcg ctcaggatgg tgttaatggt ggtgccgcag acggcgcact
     1921 tgcctttgag ggcaggcttg ccattcttca tggtgacgcg ctcggcatcc ttcatttcac
     1981 gtttctcttt acatttcaca caatagtact cggacatgtt atggttcctc cttacgtgct
     2041 gtgtacgtgt tgcttgaata aacgtcgatg tttcccttgt ctggatagac tgcccgcgac
     2101 aggtatacct cgtatgcctg tgcctgcaat cggtcccacg cttgcattgc gtgggagaga
     2161 tcggaaaatg gcgcatagag cgtcggcccg ctgccggaca gccgtaccag ctcgcacccc
     2221 gccgccaaca agtcggctct tgcctggaca acctctggat gtagctccag ggtagcg
//
LOCUS       DASZAK010000620         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_620714, whole genome shotgun sequence.
ACCESSION   DASZAK010000620 DASZAK010000000
VERSION     DASZAK010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_620714"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VGT44_10895"
     CDS             complement(<1..784)
                     /locus_tag="VGT44_10895"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV2581347.1"
                     /translation="MNQHALTALRAWMAAQGVDAFLVTQEQNRSYLSGWLCDDNEGAG
                     MLLVGQQQQVLLTSTLYREIAEQEAVGWQIVVPEGREFAPALVELAKEHGWKRIGFES
                     SAMTVAAFERLLAAGQDVFTLQSFEESYVSAMRQVKQPQEIDRLRRAIEITDQTFTHL
                     CRWIEPGMTEKEIAWEISRYMVELGADGISFDPIVASGPHGSMPHAEPGQRRIERGEL
                     ITIDMGARFQGYCADMTRTICLGEPKEPRMTEVFDAVLNAMRV"
     gene            944..2104
                     /locus_tag="VGT44_10900"
     CDS             944..2104
                     /locus_tag="VGT44_10900"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEV2581348.1"
                     /translation="MQLSNLINSLPPYHFADAKKKIAERIAAGVDVINLSMGDPDLPA
                     PRPVIERLCREMEHPENHRYPEYFGMRALHAAFASWFARRFDVRLVPERDILPLLGSK
                     EGLVYAAAAVLNAGDVALIPDPYYPVYITATTSVGAAPYMLPLLEENGYLPDLESIPA
                     QTLAKARLLWLNYPNNPTAACASRDFFERAVALARRHNLVILHDMAYAEVYFDDSQPL
                     SILQIPGASEVAVELHSMSKTYNMAGFRVGMLVGNPELVNAVGRLKSNIDSGMFRPIQ
                     YAAIEALQLPETWIAERNAIYRRRRDLLVAGCNAIGMRTHNTQAGLYIWAAVPQGRSS
                     RDFANWLFDTTGVYLTPGSIFGRAGEGYVRISLTAPEERIETALKRMKEAFV"
BASE COUNT          437 a          672 c          591 g          411 t
ORIGIN      
        1 agacccgcat tgcgttcaga accgcgtcga acacctccgt catgcgcggc tctttcggct
       61 cgcccaggca gatcgtgcgc gtcatatcag cgcaatagcc ctgaaaacgc gcgcccatgt
      121 caatcgtaat cagctcgcct cgttcgatac gccgctggcc aggttctgcg tgaggcatgg
      181 aaccatgcgg gccggaggcg acgatcgggt cgaatgagat gccatcagcg ccaagctcaa
      241 ccatgtagcg cgagatttcc cacgcgatct ctttctcggt catgccaggt tctatccagc
      301 ggcagagatg cgtaaaggtc tgatcggtaa tctcgatggc gcgtctgagc ctgtcgattt
      361 cctgcggctg cttgacctgg cgcatcgcgc tgacatagct ctcctcgaac gactgcagtg
      421 taaagacatc ctgccccgca gccaatagac gctcaaacgc ggcgaccgtc atggcgcttg
      481 actcaaagcc gatgcgcttc cagccatgct ctttcgccag ttccacaaga gccggggcaa
      541 attctcgccc ttcgggaacc actatctgcc agccgaccgc ttcttgctcg gcaatttcac
      601 gatacagcgt gctggtaagc aacacctgct gttgttgccc aacgagcagc atccccgctc
      661 cttcattatc atcgcagagc caaccgctca aatacgagcg gttctgctcc tgcgtcacca
      721 gaaaagcatc aaccccctgc gcggccatcc acgcgcgcag agcggtgaga gcgtgttgat
      781 tcattggata ccttctttag cagcgtgatt ttctgcaggt atcttatcat actctggaag
      841 ctaaggaaac ccgtaagagc cgatcctggt cgtaaaaatg cagaatcgcg tccctacgac
      901 gtgatatcat atcatcgtga cacaactaca aaggagccgt accatgcaac tctcgaatct
      961 catcaatagc ctgccgccct atcattttgc cgatgcgaag aagaaaattg ccgaacgtat
     1021 cgccgccggg gtggatgtca tcaacctgtc gatgggcgac cccgacctgc ccgcgccgcg
     1081 gccggtgatc gagcgcctgt gcagggagat ggaacacccg gagaaccatc gctacccgga
     1141 atattttggg atgcgcgccc tgcacgcggc cttcgcaagc tggttcgcgc gccgcttcga
     1201 tgtgcgactc gtgcccgaac gcgatatctt gcctctgctc ggctcgaaag aggggttagt
     1261 ctacgcggcg gcggcggtgc tgaatgccgg ggatgtggcg ctgatccccg acccgtatta
     1321 cccggtctac attaccgcga ccacctcggt cggagccgcg ccatacatgc tgcccctgct
     1381 ggaagagaac ggctacttgc ccgacttgga gagcatcccg gcgcagacgc tggcgaaggc
     1441 gcgcctgctc tggctgaact accccaacaa cccgacagcg gcctgcgcca gccgcgactt
     1501 cttcgagcgc gccgtggcct tagcgcgcag gcacaacctc gtcatcctgc acgacatggc
     1561 ctacgccgag gtctattttg acgactcaca gcccttgagc atcctacaaa ttcctggcgc
     1621 aagcgaggtc gccgttgaac tgcattcgat gagcaagacc tacaacatgg ccggcttccg
     1681 cgtggggatg ctggtgggca accccgaact ggtcaatgca gtggggcgct tgaagagcaa
     1741 catcgacagc ggcatgttcc ggcccatcca gtacgccgcg atcgaggcgc tgcaactgcc
     1801 ggagacctgg atcgcggaac gcaacgccat ctaccgccgc cgccgcgatc tgctggtggc
     1861 gggctgtaac gccatcggga tgcgcacgca caatacccag gcaggactct acatctgggc
     1921 agccgttccg cagggccgca gctcgcgcga cttcgccaac tggctgttcg atacgaccgg
     1981 agtctatctg acgcccggct cgatctttgg cagagctggc gagggatacg tgcgcatctc
     2041 gttgaccgcg ccggaagagc gcattgagac ggcattgaaa cgcatgaaag aagcgttcgt
     2101 atgaatagtt c
//
LOCUS       DASZAK010000621         2356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_50296, whole genome shotgun sequence.
ACCESSION   DASZAK010000621 DASZAK010000000
VERSION     DASZAK010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50296"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VGT44_10905"
     CDS             complement(<1..538)
                     /locus_tag="VGT44_10905"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2581349.1"
                     /translation="MAEMLPIVDAHVHLWNPELFSMPWLADIPMLNRAYDVRDYRAQT
                     QGLPITGMVYVEVGVEPQEALLEARHVVELAGEEPRLQAVVAAAPVELGEAVREHLES
                     LVAISPLIKGVRRNIQDEVEPDFCLRPDFVAGVRLLTEYHLSFDLCIKHWQLPAVVEL
                     VRRCPQTDFVLDHVGKPDI"
     gene            complement(558..>2356)
                     /locus_tag="VGT44_10910"
     CDS             complement(558..>2356)
                     /locus_tag="VGT44_10910"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEV2581350.1"
                     /translation="GGGGPTEEQMERMQIMADLPDFPQVRAGRAGDYFTDLYQRVWEN
                     PRLPTWVGELYLEYHRGTYTSQAHIKQANRRAELFYREVELLNAWASLYGMPSRQAEL
                     NQGWRLILLNQFHDVLPGSSIREVYDDAERIYAEAHAIGKRIYDEALTILLQSIRAGE
                     QDLLILNTLAWERTDPLQAPAALAPALPHAQQTTDWEGNTVLLLDGVRLPPCGAQAVS
                     ATSDAGIGEAQISSTPRAVYADEETIVLQNEYYDLWIDAQGEISRLYDRHAERDILAP
                     GKTGNQLIAYEDRPLNFDAWDIDLFYEEKPYPVRAVISMRIIEEGPVRATVEIVRPYM
                     SSLITQRISLWRNSPRIDIATEIDWHEHQTLLKAAFPLAINSTRATYEIQFGNVERPT
                     HRNTSWDMARFETCAHRWIDVSEGGYGISLLNDSKYGHDVHDNVVRLTLLKSGVFPDV
                     QADEGLHRFTYSLLPHGGDWREAQSVRRAYELNVPVVCVAGQKRATSLPSVSATGVTS
                     SSFVQVDCAHTVLETVKPAEDGDGIIVRLYEAHNQRGRGTLTFATSLLSAQECNLLEE
                     PINDVSYQGTALSFQVRPFEIKTFRIHLSPQG"
BASE COUNT          524 a          681 c          648 g          503 t
ORIGIN      
        1 tgatgtccgg cttcccaaca tgatccagga cgaagtcagt ttgcgggcag cgccgcacca
       61 gttcaacgac agcgggcagt tgccagtgtt tgatacagag gtcaaacgac agatgatact
      121 ctgtcagcag gcgcaccccc gccacaaaat cgggccgcaa gcagaaatcc ggctcaactt
      181 cgtcctggat gttgcgacga acccccttta tgagcggcga gatggcgacc agggattcca
      241 ggtgttcgcg aacggcttca ccaagctcga ccggcgctgc ggcaacaacc gcttgcaagc
      301 gcggctcctc accggcaagc tccacgacat ggcgcgcctc aagtaacgcc tcctgcggtt
      361 cgacccccac ctctacatag accatacccg tgatagggag tccttgcgtt tgtgcgcggt
      421 agtctcgcac gtcataggct cgattgagca tgggaatatc cgccagccag ggcatagaga
      481 atagttcggg attccagaga tgcacgtgcg catccacaat aggaagcatc tcggccatag
      541 acaggtcctc ccctgagtta tccctgcggg gatagatgga tgcgaaatgt ttttatctca
      601 aaaggtctta cttgaaatga tagcgcagtc ccttgatagg agacatcgtt gatcggttcc
      661 tctagaaggt tgcattcctg cgcggaaaga agcgatgtag caaacgtgag tgtgccacgc
      721 ccgcgctgat tatgcgcttc gtacagccgt acaatgatac catccccatc ctcggcaggt
      781 ttcaccgtct ccagtacagt atgcgcgcaa tcaacctgta caaatgaaga gcttgtgacg
      841 cctgttgcag agacgctagg aagagacgta gccctcttct gcccggctac gcacaccacc
      901 ggaacattga gttcgtaggc gcgccgcacg ctctgcgcct ccctccaatc tcctccatgc
      961 ggcaacaggc tataggtgaa gcgatgcaag ccttcgtccg cttgaacgtc agggaagact
     1021 ccggatttga ggagcgtcag acgaacaaca ttatcatgaa catcgtggcc atacttgcta
     1081 tcgttaagca ggctgatacc atatcccccc tcgctcacat cgatccaacg atgcgcgcag
     1141 gtctcaaaac gggccatatc ccaggatgtg ttacggtgcg tggggcgctc aacgttgcca
     1201 aattggattt cataggttgc gcgcgtgctg ttgatcgcta gcggaaacgc cgctttcagc
     1261 aaggtctggt gttcgtgcca gtcaatctcg gtagcaatgt cgatacgcgg cgagttgcgc
     1321 cagagcgaaa tacgctgcgt gatgagcgag gacatatagg gacggacaat ttcgacggtg
     1381 gcacgcaccg gtccctcttc gataatgcgc atcgatataa cggctcgtac cgggtacggc
     1441 ttctcctcat aaaagagatc tatatcccag gcgtcgaaat tcagggggcg atcctcatag
     1501 gcgataagct ggttgcctgt tttgccaggg gcaagaatgt cacgctctgc atgcctgtcg
     1561 tagagccgac tgatctcgcc ctgagcatcg atccagaggt cgtaatactc attctgcaag
     1621 acgattgtct cttcatcagc gtagacagcg cgaggagtag acgatatctg tgcctcaccg
     1681 atccccgcgt cgctggttgc cgatacggcc tgcgctccac atgggggaag gcgcacgcca
     1741 tccagcaaga gcaccgtgtt cccctcccag tcggtcgttt gctgggcatg cggaagagcc
     1801 ggcgccagcg cagccggcgc ctgtaagggg tccgtgcgtt cccatgccag ggtatttaag
     1861 atgaggagat cctgctctcc cgcccggata gattgcagca aaatagtaag cgcttcatcg
     1921 tatatgcgct ttcctatggc gtgtgcctcg gcgtagatgc gttcggcatc gtcatagact
     1981 tcgcggatcg acgagccggg caagacatca tgaaattgat tcaggagaat gagccgccac
     2041 ccttgattca gttccgcctg gcgggagggc atgccgtaga gactggccca ggcattcagc
     2101 aattccacct cgcggtaaaa taactcagct cgccggttag cctgtttgat gtgcgcctgc
     2161 gaagtatagg tcccccggtg atactctaag tacaactcac ctacccatgt gggcaaacgc
     2221 gggttctccc acacgcgctg gtagaggtcc gtgaagtaat ctccagctcg gccggcacga
     2281 acctggggaa agtccggcag gtcagccatg atctgcatcc gttccatctg ttcttccgtt
     2341 ggtccaccac caccat
//
LOCUS       DASZAK010000622         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_548044, whole genome shotgun sequence.
ACCESSION   DASZAK010000622 DASZAK010000000
VERSION     DASZAK010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548044"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VGT44_10915"
     CDS             complement(<1..1141)
                     /locus_tag="VGT44_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2581351.1"
                     /translation="MYYKFDVVIVGAGGAGLMAAMQLPHASVAVLSKVYPTRSHTGAA
                     QGGVAAALGNTEEDHWKWHMYDTVKGGDYLVDQPAAEILAREAIDAVYELEHRGLPFN
                     RTEDGRIDQRRFGGHTRNYGEAPVRRACYAADRTGHMILQTMYQNAIKNQVRFFNEFL
                     VLDLLLNDGQACGVVALEIRTGEIHTFHASAILFATGGYGRAWRVTSNAFACMGDGMA
                     IAYRRGIPLQDMEMYQFHPTGIYKVGVLLSEAARGEGGRLLNGKGEYFMERYMPTLKD
                     LAPRDIVSRCIIQEIKEGRGVDGKDYVYLDVRHLGAKVISEKLPDITDFARNYLGVEP
                     ITEPVPIQPTAHYAMGGIPTDVDARVVIDPQWTPLPGFYAAGEVAC"
     gene            complement(1169..1579)
                     /gene="sdhD"
                     /locus_tag="VGT44_10920"
     CDS             complement(1169..1579)
                     /gene="sdhD"
                     /locus_tag="VGT44_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEV2581352.1"
                     /translation="MRVSAGYGSRPASGGFETFSWYFFRITGVALIFLAIIHVFLNHV
                     TTDVACTSYQLIAARYHNPFWRLYDWLLLTLALLHGMNGLRVVIDDYVRARGWRLTLQ
                     STVGVLTLTFFLLGTITLVTFQPVVGSLGPNCIK"
     gene            complement(1604..1984)
                     /gene="sdhC"
                     /locus_tag="VGT44_10925"
     CDS             complement(1604..1984)
                     /gene="sdhC"
                     /locus_tag="VGT44_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEV2581353.1"
                     /translation="MYKGQSGMWSWLLHRVAGLGILLFLLVHIVDISLLGFGPNVYNE
                     GIALFGTAIVRVLSLGLIAGLLYHSFNGVRIMLIDFWPKGARYQRAMFATVMVLTIVC
                     FLPMAYFVIAPVFGINAGNTASGM"
     gene            2193..>2384
                     /locus_tag="VGT44_10930"
     CDS             2193..>2384
                     /locus_tag="VGT44_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581354.1"
                     /translation="MVDEPGRIMRMLTTPSINVQTWKQAPCRACMVLLTLYVYYTPGW
                     GKAQTWKAGLAQYHLKTWSL"
BASE COUNT          578 a          668 c          640 g          498 t
ORIGIN      
        1 cgcaagctac ctcaccggcg gcatagaagc ccgggagcgg agtccactgg ggatcgatca
       61 cgacgcgagc gtcgacgtcg gtcgggatgc cacccatcgc atagtgtgcc gtaggttgta
      121 tcggcacagg ctccgttatc ggctcgacgc cgaggtagtt acgcgcgaag tcggtaatat
      181 ccggcagctt ttcgctgatt acctttgctc ccaggtgccg cacatccagg tacacatagt
      241 cttttccatc cacaccgcgc ccctctttga tttcctgaat gatacagcgc gagacgatat
      301 cgcgcggcgc caggtctttc agggtgggca tgtagcgctc catgaagtat tcacccttgc
      361 cattgagcag tctgcctccc tctccacgag ctgcttcgct caagagcaca cctaccttgt
      421 agatgccggt gggatggaac tggtacatct ccatgtcttg cagggggatg cccctgcgat
      481 aggcgatggc catgccgtca cccatacagg caaaggcatt gctggtgacg cgccaggcac
      541 gcccatagcc accggtagca aagaggatgg ctgaggcatg gaacgtatgg atctctccgg
      601 tgcgaatttc gagcgcgaca acgccacatg cctgcccgtc attcaacaac aggtccaata
      661 ccaggaattc attgaagaag cgcacctggt tcttgatggc gttctggtac atggtttgaa
      721 ggatcatgtg cccggtacgg tcagcggcat agcaagcacg acgtaccggc gcctctccat
      781 agttgcgtgt gtgcccacca aagcggcgct ggtctatgcg cccatcctcg gtgcgattga
      841 agggtaggcc gcgatgctcc agttcgtaca cggcatcgat agcttcccgc gccagtatct
      901 ccgccgcggg ctgatctacc aggtaatcgc cgcccttgac ggtatcgtac atgtgccatt
      961 tccagtgatc ttcctctgta ttgcccaacg ccgcggccac accgccctgg gccgcgcccg
     1021 tgtgcgagcg cgtgggatac actttgctga gcaccgcaac gctggcatgc ggaagctgca
     1081 ttgcggccat cagcccggcg ccacctgcgc cgacgatcac cacatcgaat ttataataca
     1141 tgtctctatc cttacctatc ctggtccatt acttgatgca attggggccg agactcccga
     1201 ctacaggttg gaacgttacc agtgtaatcg tgcctaacag aaaaaaggtc aatgttaaga
     1261 cgccgacagt agattgtaat gtaagacgcc atccacgcgc gcgcacgtaa tcatcaatga
     1321 ctacacgcag gccattcatg ccgtgcagca gagcaagcgt gagcagcagc cagtcgtaca
     1381 gccgccagaa aggattgtgg taacgtgccg cgatcaattg ataactggtg caggcaacat
     1441 ctgtcgtcac atgattaagg aacacgtgaa taatggctag aaatatgagc gcgacgcctg
     1501 taatacggaa gaaataccag gaaaacgttt cgaaaccgcc acttgccggg cgtgaaccgt
     1561 aacccgccga aacgcgcata actgttctcc tatctacgag tttctacatg ccagaagctg
     1621 tattaccagc attgatacca aagaccggcg caattacaaa gtaggccatg ggaaggaagc
     1681 agacgatagt gagtaccatc accgtggcaa acatggctct ctggtagcga gccccctttg
     1741 gccagaagtc aatcagcatg atgcgcacgc cgttaaagga atggtacagc agaccggcaa
     1801 tcaatcctaa cgataagacg cgcacgattg cggtaccgaa gagcgcaatg ccttcgttat
     1861 aaacattggg gccgaagccg agcagcgaaa tatccacgat atggacaagt aggaacaaaa
     1921 ggatgcccag ccctgcaacg cggtgcagca gccaggacca catacctgat tgacctttat
     1981 acataacgct cctcaattct cgaggcggga ggcgggggct tcaggggtat gccttgcggg
     2041 cgcccaaata aaaactcggg ccataggagc atcccttgct tagtggtacc cgaccctctc
     2101 gccttatcgt tccgggcatt cacaaatcgg gaatgccaca ctaaatattt gacaggcgaa
     2161 aagctcgtta gtcccgcaag aagtggatgc acatggttga tgaacctggt aggatcatgc
     2221 gtatgctcac aactcctagt atcaacgtac aaacctggaa acaggcacct tgtcgggcct
     2281 gcatggtttt gttgacgttg tatgtttatt atactccagg gtgggggaag gcgcaaacgt
     2341 ggaaggctgg actcgctcaa tatcatttga aaacctggag cttg
//
LOCUS       DASZAK010000623         2266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_443107, whole genome shotgun sequence.
ACCESSION   DASZAK010000623 DASZAK010000000
VERSION     DASZAK010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443107"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2042)
                     /locus_tag="VGT44_10935"
     CDS             complement(<1..2042)
                     /locus_tag="VGT44_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEV2581355.1"
                     /translation="MRQRLPMLHKSDATRLSPPTADTQALAHDLRASMRGEVRFDEGS
                     RALYATDGSNYRQVPIGVVLPRSIEDVIQTVALCKRHAVPLLPRGGGTGLAGQTCNTA
                     VIIDMSRHMRAILDLDPGRKIARVQPGVVLDVLRNAAERSRLTFGPDPSTHAQCTLGG
                     MIGNNSCGTHSVMAGKTSENVESLDILTYDGLRLRVGATSDDELAQIIREGGRRGDIY
                     ARLKTLRDTYADLVRARFPNIARRVSGYNLDQLLPENGFHVARALVGSEGTCVTVLEA
                     TVRLVSSPPARALLVLGFSDAFIAADYVPEIMAHRPIALEGFDSALIENMRKKNLHPA
                     EAALLPEGGGWLLVEFGADTPQACLEQAQPLIEQMRHKAHPPPIKLFDQPREAAKIWM
                     VRESGLGATAIVPGEHHHWEGWEDAAVPPEKLGDYLRDYSRLVKRFGYTTAFYGHFGQ
                     GCVHNRIDFDLASHEGIVKFRAFMEEATEMVVGYGGSISGEHGDGQARGELLPRMFGP
                     ELMQAFREFKAIWDPEGKMNPGKLIDAYPLDEHLRLGASYNPAQPATHFSFADDEGSF
                     AAAALRCVGVGKCRRVDGGTMCPSFMVTHDEEHSTRGRAHLLFEMLQGQVLTDGWRNE
                     HVKQALDLCLACKGCKSDCPVNVDMATYKAEFLSHYYQGRLRPMSAYTMGLIFRWAR"
BASE COUNT          397 a          673 c          760 g          436 t
ORIGIN      
        1 cgcgcccagc ggaagatgag gcccatcgtg taggcgctca tgggacgcag cctgccctgg
       61 taatagtgag aaagaaactc ggccttgtag gttgccatgt caacgtttac ggggcagtcg
      121 ctcttacagc ccttacaggc caggcacagg tcaagcgcct gcttgacgtg ttcgttacgc
      181 cagccgtcgg tgaggacctg gccttgcagc atctcgaaga gcaggtgtgc gcgcccgcgt
      241 gtgctgtgtt cttcgtcgtg cgtgaccatg aagctcggac acattgtgcc tccatcaacg
      301 cggcggcact tgcccacgcc gacacagcgc agagcagcag cggcgaaact gccctcgtcg
      361 tcggcgaagc tgaaatgcgt cgcgggctgg gccgggttgt acgaagctcc gaggcgcagg
      421 tgttcgtcga gggggtaggc gtcgatcagt ttgcccggat tcatcttgcc ctccgggtcc
      481 cagatggcct tgaattcgcg aaacgcctgc atgagttccg ggccgaacat cctgggcagc
      541 agttccccac gcgcctgccc gtctccatgc tctcctgaaa tggagccgcc atagccgacc
      601 accatctcgg tcgcctcctc catgaaggcc cggaacttga cgatgccctc gtggctggcc
      661 aggtcgaagt cgatacggtt gtggacgcag ccctggccga agtggccgta gaaggcggtg
      721 gtgtacccaa agcgtttgac cagccgggag taatcgcgca ggtagtcgcc cagcttttcg
      781 ggcggcacag ccgcgtcctc ccagccctcc cagtggtgat gctcgccggg gacgatggcg
      841 gtcgcgccca gaccagattc tcgtaccatc cagattttag ccgcctcccg cggttgatcg
      901 aaaagtttga tgggtggagg gtgcgccttg tgccgcatct gctcgatcag gggctgcgcc
      961 tgctccaaac atgcctgcgg cgtgtccgcg ccgaactcga ccagcagcca gccacctccc
     1021 tccggcagga gggctgcctc ggcgggatgc aggtttttct tgcgcatatt ctcaatcagg
     1081 gctgagtcga agccttcgag cgcgatgggc cggtgagcca taatttcggg gacataatcg
     1141 gcggcgatga aggcgtcgga gaagccgagc accagcagcg cgcgagcggg agggctggag
     1201 acgaggcgca cggtggcttc cagcacggtg acgcaggtcc cctcgctgcc gaccagcgcc
     1261 cgtgcgacgt gaaagccgtt ttctggcagc aactggtcga ggttgtagcc ggagacgcgg
     1321 cgggctatgt tggggaagcg cgcgcgaacg aggtcggcgt aggtgtcgcg cagggtcttg
     1381 agacgcgcat agatgtcgcc gcgccgcccg ccttcgcgaa tgatctgcgc gagttcgtcg
     1441 tcactggtcg cgcccacgcg caggcgcagg ccgtcgtagg tgaggatatc cagtgactcg
     1501 acgttctcac tcgtcttgcc tgccatgacg gaatgggtgc cgcaggagtt gttgccgatc
     1561 atgccgccca gcgtgcattg cgcgtgggtt gaaggatcgg ggccgaaggt gaggcgcgag
     1621 cgctcggcgg cgttgcgcaa cacatccagg acgacgccag gctggacgcg agcgattttg
     1681 cgcccaggat cgagatcgag gatggcgcgc atatgcctgg acatatcgat gatgacggcg
     1741 gtattgcagg tctggccggc caggccggtg cctccgccgc gcggcaggag cgggacagca
     1801 tgacgcttgc agagcgcgac ggtctgaatg acatcctcta tgctgcgggg cagcacgacg
     1861 ccgatgggta cctgccggta gttggagcca tcggtggcgt agagcgcccg gctgccttca
     1921 tcaaaacgca cctcgccgcg catgctggcg cgcaggtcgt gcgccagggc ctgcgtatcg
     1981 gcagttgggg gtgatagtct tgtggcgtct gatttgtgga gcatgggaag tctctgtcgc
     2041 attgctcttg tctccttgtg agtcgtggtt ggcaaaaata gcgtcaacta gcgcgtctac
     2101 ctctgtaggt gccgatttat cgtgcacacc gccgatttat cggcctgaat gcccgccgtt
     2161 gacgctcatc ggggccgata aatcggcggt gtgcacgata aatcggcacc tacagaggta
     2221 gacgcgctag ttgacgctat ttttgccaac cacgactcac aaggag
//
LOCUS       DASZAK010000624         5936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_584624, whole genome shotgun sequence.
ACCESSION   DASZAK010000624 DASZAK010000000
VERSION     DASZAK010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584624"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..979
                     /locus_tag="VGT44_10940"
     CDS             260..979
                     /locus_tag="VGT44_10940"
                     /inference="COORDINATES: protein motif:HMM:NF044575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581356.1"
                     /translation="MSKSELPSKQAEQQVRQGEFDRSPNIQWHAKHQLPNNPTLEQRV
                     QWHMAHARRCPCASQDEDILEDLKKSYVGTHQDFWIWYTTNDHRALGLWAADCAEHLL
                     PYFEETYPEDPRPRDAIRTLRAWVQTGEFRMAIIRKASLDAHAAAKAVKQEEQVARFA
                     AHAAGQAVGTAHVPTHALGPVLYALKLVAAVHSTDVKAAVAQERAWQTQRLPEHLRPW
                     VDAWVEKTYPLLPKQLRMQLD"
     gene            complement(1114..2151)
                     /locus_tag="VGT44_10945"
     CDS             complement(1114..2151)
                     /locus_tag="VGT44_10945"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2581357.1"
                     /translation="MSKSAVERPVEEPGQTISGTLRRQGRQIVASWIRELALLPVIVL
                     LVIVGAFVNSSFLTANNFINVAQESAALGAVVVAETLILLTGKFDLSLQSTYGLAPMV
                     GAWLIVSKEGQGLGINWNPLVGIAVVLLIGLVVGAFNGFMVIKLGFNAFIFTLAMLIL
                     LHGIQVGIVNGRTIYFLPDTFTYLGSQYLLGIPVSVWITGAIFLVAALFLRYHRVGRA
                     IYAIGGNTEAARAAGIQVDRIRIGVFIVAGLLAALAGLMTAGEVVAVTASQGDNLIFS
                     VFAAAVIGGISLDGGRGRMVGALTGVILLALVQNILILSQIQTFWIDAANGLVILVAL
                     ALARLIGSERA"
     gene            complement(2168..3736)
                     /locus_tag="VGT44_10950"
     CDS             complement(2168..3736)
                     /locus_tag="VGT44_10950"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEV2581358.1"
                     /translation="MIEHENGGPAALRTSVGVAQGVWKAFGETQALRDVSLEVEAGEC
                     HALVGRNGAGKSTLVAILTGLIRPDRGFVRLGAEAAPTLADRAAWQRLVACVYQKSTV
                     VPSLTVAENVFLNRATGNDHGFINWRAMRSRARQVMLDWGFDLDVNRRASDLTVEQRQ
                     VVEIARALSVGARFLILDEPTASLEKAAIERLFDSIRRLKGSGVGILYISHHLEEIYD
                     VCDRVTVLRDGERVLSTPVSEIKQDRLIEAMVGVAPQRATEIAAPTRDNTSAQPLLEI
                     SHLHVPATLGPVQDVSFSVSLGECVGLVGLRGSGTATVADAVAGLVKPASGDIKLDGK
                     AIQAGRVDATLRQGVAYVPEDRHARGFIPYLGVGANLTLSVLDRLSSWGIIARSRSNR
                     VAASLVEQLDIVTSGLDQRVGQLSGGNQQKVVVGRALARHPRVLVAVTPTVGVDVAAK
                     ESLLNVIDAAREGGTAVLLVSDDLEDLRICTRLLVMVKGKLVKEYRQPPWDRHDLIAA
                     VEGLEPASEQGESA"
     gene            complement(3792..4859)
                     /locus_tag="VGT44_10955"
     CDS             complement(3792..4859)
                     /locus_tag="VGT44_10955"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2581359.1"
                     /translation="MQSMFASRLRALYVLIVVAIVLAACGSTTGGTSTPTSGGVLAPG
                     ANFKLGVSLTFNNTDFWTNYISYETKFAAQYNATIIGPLVANNDAAQQITDIHTLIQE
                     GAQALIVNPVDSAAIAPALQFAASKNIPVVSVDVAPSSGKVFMIVRADNVAYGQNSCK
                     FIATHATAKTGHIAILEGDLASLNGQDRSNGCVQEIKTNYPNFQTVEYATKWDTPTAV
                     NDAKTALSTYSDLRGIYVEWSAPEDGIIAAEQAIGRFTKVGSPSHIVLIGNDGVPHEH
                     AQIRAGMLDATISQPANSYAQYAVFYAQQALEGKTYSANQTTDHNSTIVSLDGNLEDA
                     LPAPVVDVTNVNDPSLWGNNT"
     gene            complement(4910..5680)
                     /locus_tag="VGT44_10960"
     CDS             complement(4910..5680)
                     /locus_tag="VGT44_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV2581360.1"
                     /translation="MASLSTGTNDRKKGTVAEVFDEDLSAELFNLLWGQERTAMPTVV
                     DYAYEEIWKRVIMIGGSEEQRLSDVTLAEQLGMSRTPVRQALERLVQEGLVRSDPRRG
                     FWTSTFTAQDINEIYDLRGALETLAVRLAAPHLNQEDLKAHLEALLAVRAKLDTHPVL
                     PFLQVDIRFHMLITRASNNGRLIHSLSLLRSQLSMFQMQDTLYPKRMEIALNDHEQIL
                     LALLAGNVDEAADCLARHIRHAKEGVLADIFLEGKEGV"
BASE COUNT         1261 a         1759 c         1705 g         1211 t
ORIGIN      
        1 ccggcggcag ctcgttcggt gatgtccagc cactctggga aagtggtcgt ctgtccgcgc
       61 cgtagcatag agcctccaat cgtactgcct ccagcttgat ccggtgaacg ttcgagccgg
      121 agtaactgag ccgctaccgg ttgcggctcc cagggacgat tgtatgaggc tttggacaaa
      181 tacgctcggc acgacagacc gagacgcaac cgagtccagc aagttcgcag catttccctt
      241 ctcggggtat actacctcta tgtccaagag tgagctacct tcaaaacaag cagaacagca
      301 ggttcggcag ggagaatttg atcgttctcc caatatccag tggcatgcga aacatcaact
      361 gcccaacaat ccgaccctgg agcagcgagt gcaatggcac atggcacatg ccagaagatg
      421 cccctgtgcg tctcaggacg aggatatact ggaagacctg aagaagagct atgtggggac
      481 ccatcaggac ttttggattt ggtatactac caacgaccat cgggcgttag gtctctgggc
      541 agcagactgt gcggaacatc ttttgcccta cttcgaggaa acatacccag aagaccctcg
      601 tcccagagac gcgatcagaa cactgcgagc ctgggtccag accggcgagt tccgtatggc
      661 aatcattcgc aaagcatcgc ttgacgctca tgcggcggcc aaagccgtca agcaagaaga
      721 gcaagtcgca cgctttgccg cgcatgccgc cggtcaagct gtgggcaccg cgcatgttcc
      781 cacccatgcg ctcggtccag tgctttacgc tctgaaactg gttgctgctg tacattcgac
      841 cgatgtcaaa gcggccgtcg cacaggaacg agcgtggcaa acgcaacgcc ttcctgaaca
      901 tcttcggccc tgggtcgatg cttgggtcga aaaaacgtac ccccttcttc ccaaacagct
      961 tcgtatgcaa ttagactgaa agggcaggct gacaaagttt tattgagtct cagtacgacc
     1021 tggccggtac gactttgtga gactttcact aagaagggcg gtacgacatt gatgaatcgg
     1081 aaaaacgaag ttttattcag cgatcaacag ttgttatgct cgctcggagc cgattaagcg
     1141 ggccagcgcc agggcgacca ggatgacaag tccgttggcg gcgtcgatcc agaacgtctg
     1201 aatctgggaa aggatcagga tgttttggac gagcgccagc aggatgacgc cggtgagcgc
     1261 gcctaccatg cgcccgcggc ctccatcgag agagatgccc ccgatcacgg cggccgcaaa
     1321 gacagagaag ataaggttat cgccctggct ggcggtaacg gccaccacct cgcccgccgt
     1381 catcagtccg gcaagtgcgg ccaggaggcc ggcgacgatg aagacgccga tgcggatgcg
     1441 gtctacctgg atgcccgctg cccgcgctgc ttcggtgttg ccgccgatgg cgtagatggc
     1501 gcgtccaacg cggtgatacc gcaggaagag cgccgcgacc aggaagatcg cgccggtgat
     1561 ccagacggag acgggtatgc ccagcagata ctgcgagcct aaataggtga acgtatcggg
     1621 caggaagtag atggtgcgcc cattcacgat tcccacctga atgccgtgca ggaggatcag
     1681 catggccagc gtgaagataa aggcattgaa gcccagcttg attaccatga agccgttaaa
     1741 ggcaccgacg accagtccga tcagcaggac gaccgcgatc ccaactaatg ggttccagtt
     1801 gatacccagg ccctgacctt ctttcgagac gatcagccag gcccccacca tcggcgccag
     1861 gccatacgtc gattgaagcg acaggtcaaa cttgccggta agtaggatca gagtctcggc
     1921 caccacaaca gcgcccaacg cggcagattc ttgcgcgacg ttgatgaagt tgttcgcagt
     1981 cagaaaggac gaattcacaa aagcgccgac gatcacgagc aggacgatca ccggaagcag
     2041 ggcgagttct cgtatccagc tggctactat ttgccgtcct tgacgccgca gcgtaccgga
     2101 gatcgtctgc cccggctctt caaccgggcg ctctactgcc gatttactca ttccaattcc
     2161 cctttactca tgccgattct ccttgctcgg aggccggttc gaggccttcg actgcggcga
     2221 tcagatcgtg ccggtcccag ggcggctgac gatactcctt gaccaatttc cctttgacca
     2281 tcacgagcag ccgtgtgcag atgcgcaggt cctccaggtc gtcggagacg agcagaaccg
     2341 ccgtgcctcc ttcgcgcgcc gcgtcgatca cattgagcag cgattctttg gcggccacgt
     2401 caacaccaac cgtcggtgta accgcgacaa gtacccgcgg atgccgggcc aacgctcgcc
     2461 cgacaacgac cttctgctgg tttcccccac tcaattgtcc gacgcgctgg tcgaggccgc
     2521 tggtgacaat gtccagctgt tcgacaaggc tggcggcgac ccggttactg cgggagcgcg
     2581 caatgatccc ccaactcgat agacggtcga gaacagataa cgtcagattg gctcccactc
     2641 cgaggtacgg aatgaagccg cgcgcatgac gatcctcagg tacgtaggca acgccttgtc
     2701 ggagggtggc atccacgcgg cccgcctgga tcgctttgcc atccagcttg atgtcgcccg
     2761 atgctggctt caccaggccg gctacggcgt ccgcaacggt tgcggtgccg gagcctcgca
     2821 atccgaccag gccgacgcac tcgcccaggc tcactgaaaa gctcacatcc tgcaccgggc
     2881 ctagcgtggc cggaacatgt agatgtgaaa tttcgagcag cggctgtgcc gatgtattgt
     2941 ctcgcgtcgg agcggcgatc tccgtagccc gctgtggggc gaccccgacc attgcctcga
     3001 tcaagcgatc ctgcttgatc tcgctgaccg gtgtgctcag cacccgctcg ccgtcacgca
     3061 gcacggtcac gcggtcgcac acatcataaa tctcctcgag atggtgggag atgtagagga
     3121 tgccgacccc gctccctttg agccggcgaa tgctgtcaaa cagacgttcg atggcagcct
     3181 tttccagcga tgccgtcggc tcgtcgagga tcaggaagcg cgcgccgacc gaaagggcgc
     3241 gcgcgatctc gaccacctgg cgctgctcga cggtgaggtc gcttgcgcgg cggttgacat
     3301 ccaggtcaaa gccccaatcg agcatgacct ggcgcgcgcg ggaacgcatg gcgcgccagt
     3361 tgatgaagcc atgatcgttc ccggtagcgc ggttgagaaa gacgttctcg gcgaccgtca
     3421 ggctgggtac caccgtcgat ttctggtaga cgcacgcgac caggcgctgc caggcggcgc
     3481 gatccgcaag ggttggcgcc gcttcggctc cgagcctgac aaagccgcga tcaggccgga
     3541 tcaggccggt caggatggct accagcgtgg atttccccgc gccgttgcgc ccaaccagcg
     3601 catggcattc tccggcctcg acttcgagag acacgtcgcg aagcgcctgg gtctcgccga
     3661 aggctttcca gacgccctgc gccacgccaa cggaggtccg gagcgccgcc ggccctccgt
     3721 tttcatgttc tatcacaaat cctccgaata caaattctat cacattccag gtgtgatgcg
     3781 cgggtgctga actacgtgtt gttgccccac aggctcggat cgttgacgtt ggtgacgtcc
     3841 acgacaggcg ccgggagcgc gtcctcaaga ttgccgtcca gactgacgat tgtgctgttg
     3901 tggtcggtgg tctggttggc cgaataggtc ttcccttcga gcgcttgctg ggcgtagaag
     3961 actgcgtatt gcgcatagga gttggccggc tgcgaaatgg tcgcgtcgag cattccagcg
     4021 cggatctgcg catgttcatg cggcacaccg tcattgccaa tcaggacaat gtgcgatggg
     4081 ctgcccactt tcgtaaacct gccgatagcc tgttcagccg cgataatgcc gtcttcgggt
     4141 gcgctccact cgacgtagat gccccgcaga tccgagtagg tactcagcgc tgtcttggca
     4201 tcgttgacgg cggtcggagt gtcccacttc gtcgcgtatt cgacggtctg gaagttcgga
     4261 tagttcgttt tgatttcctg gacgcagccg ttcgaccggt cctggccgtt caaagaagcg
     4321 agatcccctt ccaggatcgc gatgtggccg gtcttggccg ttgcatgggt ggcgataaac
     4381 ttacaggaat tctggccata cgccacgtta tcggcgcgca cgatcatgaa taccttgccg
     4441 ctgctcggcg ctacgtctac gctgaccacc ggaatgttct tgctcgcggc gaattgaagc
     4501 gcgggcgcga tggcggcgct gtccaccggg ttgacgatca gcgcctgcgc gccttcctgg
     4561 atcagcgtat ggatgtccgt gatctgctgg gcggcgtcat tgttggcgac cagcggcccg
     4621 atgatggtag cgttgtactg agctgcgaac ttggtctcat acgagatata attggtccag
     4681 aagtcggtgt tgttgaaggt cagtgaaaca ccgagcttaa agtttgcgcc cggcgcgagt
     4741 acacctcctg acgtcggcgt tgacgtaccg cctgtcgtac tgccacaggc cgccagaact
     4801 atcgcgacga cgatgagtac atacagcgct cgaagacgac ttgcgaacat cgattgcatg
     4861 gttctctcct tctgggtatt gaattccgac acgcgaactc tatccttctt taaacgccct
     4921 cctttccttc taaaaagata tctgccaaaa caccttcctt cgcatggcgg atatgcctgg
     4981 ccaggcaatc cgccgcttca tcaacatttc ccgcgagcag agccagcagg atctgctcat
     5041 gatcgttcaa ggctatctcc atacgctttg ggtataaggt gtcttgcatt tgaaacatag
     5101 aaagctggct gcgcagcagt gaaaggctgt ggatcaggcg tccattgttc gatgcgcgcg
     5161 tgatgagcat gtgaaaacgg atgtccacct ggaggaaagg tagcaccggg tgtgtatcca
     5221 gtttcgcgcg aacggccagc agcgcctcga ggtgcgcctt gagatcctcc tggttcaaat
     5281 gaggagccgc cagccgtaca gccagcgtct ccagtgcgcc gcgcaaatca tagatttcgt
     5341 tgatatcctg ggcagtgaag gtactcgtcc agaaaccacg ccggggatca gaacgcacca
     5401 gaccttcctg aacgagacgc tcaagtgcct ggcgcaccgg cgtgcgactc attcccaact
     5461 gctcagccag cgtgacatct gaaagacgct gctcctcgct cccaccgatc atgatgacgc
     5521 gcttccagat ctcttcatag gcgtaatcga caacggtagg catagcggtg cgctcctggc
     5581 cccagagcaa attaaataat tctgcgctca gatcctcatc aaatacctct gccacggtac
     5641 ccttcttgcg gtcattcgtt cctgtagata gagaagccat caacgattac tcctggaggt
     5701 tgttcaaaac tcttgatttt ccgtgtcata tcctgtattc tgtatacaga atacaggata
     5761 cagagtaaag tatacttact caagtctgaa tagtcaaggg gaatagaaat acttgcagga
     5821 cgccgcccgc cgtaggagcc ggtttaccgc gctcaccgcc gatttatcgg cctggaacgc
     5881 tcgtcgatca agtacatgga caggctaata atgagttttc acaaacggga tcggca
//
LOCUS       DASZAK010000625         1957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_514002, whole genome shotgun sequence.
ACCESSION   DASZAK010000625 DASZAK010000000
VERSION     DASZAK010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514002"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VGT44_10965"
     CDS             <1..1279
                     /locus_tag="VGT44_10965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEV2581361.1"
                     /translation="LMCGLAPSLSMLILFRILQGAGGGGLQPTSQAILVESFEQRQQG
                     MAMAVYGMGVVVAPIIGPTLGGWITDNFSWRWIFFINVPIGILSMVMTSTMIDDPPYL
                     VRKTLKQGLKIDFIGLGLLSIGLGFGQVVLDKGQRDDWFGSNFIVWASVVSVVGLIGA
                     VFWELRQDQPMINLRLFKNLNFAMATFMMFCLGLVLYGTTVLLPVMVQSLMGYTAEWS
                     GLVLSPGAILTLFTMPLVGRLLTKVQPRWLVVTGLAILSVGMYRLSTLSLQTGFWTFV
                     FTWTISRGGLAFLFVPINVTAFSHIEREKTSSATGLINLTRNLGGSIGISLVTALQDR
                     LSQSHQSVLAGHLMPLDPSYVSRLSNLEAGLRAGGSNPVLAAQQAQAIIYRELTRQAS
                     MLAYVDVYRLLSYLCAVLIPLMFLMRRPKPRTT"
     gene            complement(1388..1663)
                     /locus_tag="VGT44_10970"
     CDS             complement(1388..1663)
                     /locus_tag="VGT44_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581362.1"
                     /translation="MFTLWIVWSALTLFVISLALIRKFTARNEDDLVHLSGSVESTIT
                     QQFAVANKLDKIDHWGKLLTVVDVAFGVVLFIVTLYITWQNSLVLEK"
     gene            complement(1770..>1957)
                     /locus_tag="VGT44_10975"
     CDS             complement(1770..>1957)
                     /locus_tag="VGT44_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581363.1"
                     /translation="SAIIIGGLVMAPSFSFGKAEYTKKEGKGCTYCHTAAGKKDLNDV
                     GKCYAEHGHSLESCGAK"
BASE COUNT          368 a          586 c          568 g          435 t
ORIGIN      
        1 gctgatgtgc ggtttggcgc ccagcttatc catgctgatc ctgtttcgca tcctgcaagg
       61 cgccggcggc ggcgggttgc agccgacctc gcaggccatt ctggtggaaa gcttcgagca
      121 aagacagcaa ggcatggcca tggccgtcta tggcatggga gttgtcgtcg cgcctatcat
      181 cggcccgact ttgggaggct ggatcactga caatttctcc tggcgttgga tcttcttcat
      241 caacgttccc atcggcattc tctccatggt gatgacgtct accatgatcg acgaccctcc
      301 ctacctggtg cgcaagacgc tgaagcaagg cctgaagatc gatttcatcg gcctcggcct
      361 gctcagcatc gggctgggct tcgggcaggt ggttctcgac aaagggcagc gtgacgactg
      421 gttcggatcg aacttcatcg tatgggccag cgtggtcagc gtagtgggat tgatcggcgc
      481 ggtgttctgg gagctgcgcc aggatcagcc catgatcaat ctgcggctgt tcaagaacct
      541 gaactttgcc atggcgacgt tcatgatgtt ctgcctcggc ttggtgctgt acggcaccac
      601 cgtgctgctt ccggtgatgg tgcaaagcct gatgggctac acggcggagt ggtccggtct
      661 ggtgctttcg ccgggcgcga ttctgacgct gttcaccatg ccgctggtgg ggcggctgct
      721 caccaaagtc cagccgcgct ggctggtggt aacgggcctg gcgattttgt cggtggggat
      781 gtaccggcta tccactttga gtctgcaaac cggtttctgg accttcgtgt ttacgtggac
      841 catctcgcgc ggcggcctgg cgttcctgtt cgttccgatt aacgtcaccg cgttctcgca
      901 catcgagcgc gagaaaacca gcagcgccac tggactgatc aatctgacgc ggaacctggg
      961 gggcagcatc gggatttcgc tggtcacggc attgcaggac cgcctcagcc agtcgcacca
     1021 aagtgtcttg gccgggcatc ttatgccact cgatccgagc tatgtatcca ggctgagcaa
     1081 tctggaggcg ggactccgtg cgggcggctc caacccggtg ctggccgccc agcaggccca
     1141 agcgatcata tatcgcgagc taacccggca ggcctccatg ctggcttacg tcgacgtgta
     1201 ccgcctgttg agctatctgt gcgcggtttt gatcccgctc atgtttctga tgcggcgccc
     1261 caagccgaga accacttaaa gaccggcagg tcgcgcaccc aacaaaaaac gcccggcgcg
     1321 cggggcacga gtttgacctc gataccggtg cgccgggcgg gcttagcaac tttgaagaac
     1381 cgagccgcta tttttcgagc accaggctgt tctgccaggt gatgtacagc gtcacgatga
     1441 acagcacgac gccgaacgca acatccacca ccgtcagcag cttgccccaa tggtcgattt
     1501 tgtcaagttt gttggcgacc gcgaactgct gcgttatcgt actctccacg gagcctgaca
     1561 gatgcactaa gtcatcctcg ttccgcgccg taaacttgcg gatcaaggcc agagagatta
     1621 caaacaacgt gagagcggac cacacaatcc aaagcgtaaa catgaattta gcctccttgc
     1681 gtacctttgt ctccagcggc gcttgcccgg ccggtcaacc acccggccgt accgcttgcc
     1741 actacctgaa ctcatcggaa aggctgggac tacttcgccc cgcagctttc cagcgaatga
     1801 ccgtgctcgg catagcactt gccgacgtca ttcaaatctt tcttgcccgc ggccgtgtgg
     1861 caatacgtgc agccctttcc ctctttcttg gtgtattctg cttttccgaa agagaaggac
     1921 ggtgccatca ccaggccacc aataatgatt gcggatg
//
LOCUS       DASZAK010000626         3535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_229034, whole genome shotgun sequence.
ACCESSION   DASZAK010000626 DASZAK010000000
VERSION     DASZAK010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229034"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..2517
                     /locus_tag="VGT44_10980"
     CDS             262..2517
                     /locus_tag="VGT44_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2581364.1"
                     /translation="MQQLIGQGGMGAVYLAQQSRPRRQVAVKVLLPTTPLKPHHLAAF
                     LERFRRETDAAASLEHPNIMPVYEYGEQDGLAYLVMPYIGGGTLRDVMEVEGQMLLPK
                     VVNYLEQLAAALDFAHARGVVHRDIKPANILITQEGRLLLTDFGLVKIVTEEGQAQVR
                     LTGAGAPVGTPDYMSPEQVMGDEVDGRADLYSLGIILFQMLTATTPFQGETPMQIAAQ
                     QLHAQPPMPRRFRAELPEAAQQVILRSLSKRPADRYASGLEMATAFRLALNTLDAHSM
                     ASRTNVFETDDRGFDSSDLLPRKGLFDPKWQTGALLPAVNAQGAAIGDFGRLPSGSVP
                     AVQPGMMGTPGMPARSTGLLSRTGMFPSIGNGGSGAMPTANMQMPGPTTPTGSLSTST
                     GAQRAAPGFIPATPQDAPTAFGQSGISQLSPSLNVSTGAYAVPGYQGASPTVKLTGPV
                     KVVQVPIAPGQYVTGLLPVPALGQPPTPVSPRDARVALLKKIALSVVILLVLVGGSIG
                     FLEVRSHLLSERNSTLTGTVVSGTPNVQATNAARATATAMANIILFDPLSHNVRGLPE
                     GTSATRAFAFKNGAYHVTNLGKQGVAVVVPYALQNVPMVYQLTMNEVRGDDTSPVNSF
                     GMIFRYNQQTKGGVTVSTFYSFEVVNTTGTSGKYWFYKYDSSAANPWTPLWSQNFGAE
                     FKEGQGPNVLNTFKVLMNGGNFSFVVNGKLVNSKPVVDTSFPAGIVGMLVNLNGTEVA
                     FSQLEIIALPH"
     gene            2668..>3535
                     /locus_tag="VGT44_10985"
     CDS             2668..>3535
                     /locus_tag="VGT44_10985"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2581365.1"
                     /translation="MDAETLVGKVFGDCLLQGLIGAGTMGVTYRAMQSHPLRQVAVKV
                     MTRTTTLEVQQQAAFQEMFRGVMARVESLRHRHIVPVYASGFSDDGLAYVVMADIQGE
                     TLEQALAARGALALPQALRYLEQIAEALDYAHTRGIVHRDVKPATIFLSADEQALVAD
                     FLLTSALADEAIAPMRLSCAGMLDYMSPELVVGKEVDGRADIYSLGALLYHMVTGQAP
                     FQGQTLMKVAAKHLKATPPSPSALRPDLPVAAEQVILKALEKRPAHRYDSAHDLARSF
                     RRAVLGEHVQVAQ"
BASE COUNT          746 a          926 c         1092 g          771 t
ORIGIN      
        1 gtttattgcc atagatgtga agaagatgtc atgatggcgg gcgagcaggc gggtctggta
       61 gcggaagatg aataaaggtt aagaattgtt tacctgagta cattactttg gtcatgttgt
      121 ccggcttcac gcattgtaag atggcgcgaa attggctaac atgtacaaac gtaggggcaa
      181 aaaaggaaca tcaggacaga agaagaggta gtttgaatga atgctgaagc cttagtaggt
      241 actgtactgg gaacttgcac gttgcaacaa ctgatcgggc agggaggaat gggggcggtg
      301 tacctggctc aacaatcgcg tccacggcgg caggttgcag tcaaggtatt gctgccgact
      361 acgccgctga aaccgcatca cctggcagcg tttctggaac gtttccggcg cgagactgac
      421 gcggcagctt cattggaaca tccgaatatc atgccggtgt atgaatacgg agagcaggat
      481 ggtctggcct accttgtgat gccctacatc ggtggtggga cgctgcgcga tgtgatggaa
      541 gtcgaagggc aaatgctgct gccgaaggtg gttaattatt tggaacaact ggccgcagcg
      601 ctcgatttcg cccacgcgcg cggcgtggtt caccgcgata ttaagcctgc caatattttg
      661 attacgcaag aggggcgttt gctgctgacc gatttcggtc tggtgaaaat agtgaccgag
      721 gaggggcagg cgcaggtacg tttgacgggc gctggcgcgc cggtcggcac accggattat
      781 atgtcgccgg agcaggtgat gggtgacgag gtagatggac gcgccgacct gtactcgctc
      841 ggcattattc tcttccagat gttgacggcg acaacgccgt ttcaaggtga aactccaatg
      901 caaatcgctg cgcagcaact tcacgcgcag ccgcccatgc cgcgccgttt ccgcgccgaa
      961 ctgccagaag cggcccagca ggtgattttg agatcgctgt cgaaacgccc ggcagatcga
     1021 tatgcgagcg gtctcgaaat ggctactgcg ttccgactcg cgctaaacac cctcgatgcg
     1081 cacagtatgg cgtcccggac caatgttttt gaaaccgatg atcgcgggtt tgacagttca
     1141 gatctattgc cgcgcaaagg attgtttgat ccaaaatggc aaacgggcgc gttgttgccc
     1201 gcggtgaacg cgcagggggc ggcgataggc gatttcggca gattgccgtc gggcagtgta
     1261 ccggccgtgc agccgggaat gatgggaacg ccaggtatgc ctgctcgttc gacaggtctg
     1321 ctttcgcgta caggcatgtt tcccagcata gggaacgggg gaagcggcgc gatgccgacg
     1381 gccaatatgc agatgccagg tcccacaact cccaccggct ctctttcaac ttcgaccggg
     1441 gcgcaacgcg cagctccggg ctttattccg gctacacctc aggacgcgcc gaccgccttt
     1501 gggcagtcgg gtatcagcca gctatcacct tcgctgaatg tatcaaccgg ggcatatgcg
     1561 gttccaggct atcagggggc ttctcccacc gtaaaattaa ccggcccggt caaggtggtg
     1621 caggtcccta ttgctcctgg tcaatatgta acaggcttgc tgcctgtccc ggcgctcggg
     1681 cagccgccca cgccagtgtc gcctcgcgat gcgcgcgtcg cgttgttgaa aaaaatcgcg
     1741 ttgagcgtgg tgatcctgct ggtcttagtc ggtggctcga tcggattcct ggaggtgcgc
     1801 tcgcacctat tgagcgagcg taacagcacg ctgaccggta cggtggtgtc cggcactccg
     1861 aatgttcagg cgacgaatgc agcgcgggcg acggcgacgg cgatggcgaa tattatcctc
     1921 ttcgatccct taagccacaa tgtgcgtggt ctgcccgaag gcactagcgc gacgcgcgcc
     1981 ttcgccttca aaaatggggc gtatcacgtc actaacctcg gcaaacaggg cgtggcagtc
     2041 gtggttccct acgccttaca aaatgtcccg atggtctacc agttgacgat gaacgaggtg
     2101 cgcggcgacg atacctcgcc cgtcaattcg ttcggtatga tcttccgcta caaccagcag
     2161 acgaaaggtg gcgtgaccgt tagcaccttc tatagcttcg aggtcgtcaa tacgaccggt
     2221 acgagcggga agtactggtt ctacaagtac gacagcagcg ccgcgaaccc ctggacgccg
     2281 ctctggagcc agaatttcgg tgcggaattc aaagagggac aggggccaaa tgtgttgaat
     2341 accttcaagg tgctgatgaa tggcggcaat ttctccttcg tcgtcaatgg caaactggtc
     2401 aactcgaagc cagtggttga tacatcgttc ccagccggta ttgtggggat gctggtgaac
     2461 ctgaatggga cggaagtggc cttctcccaa ttggagatta tcgcgttgcc gcattagtac
     2521 agcgtatact atgaccggta ctttggtaat agtagcaagg ctagtgcatt gttatctctg
     2581 ccccttgttt gtaagattgt agtatgggga tttcttcttc aagaagctaa acgagtaccc
     2641 gaaagctaca ataaggggta gattctgatg gatgcagaaa cattggtagg gaaggtgttt
     2701 ggggattgcc tgctacaggg gctgattggc gccggtacga tgggtgtgac atatcgcgcg
     2761 atgcagtctc acccgctgcg ccaggtcgcg gtgaaggtta tgacgcgcac caccacgctt
     2821 gaggttcaac agcaggccgc cttccaggaa atgtttcgcg gcgttatggc gcgcgttgag
     2881 tcattgcgac accggcatat tgtacctgtc tacgcttctg gctttagcga tgatggcctg
     2941 gcctatgttg taatggctga tatacagggg gagacgctgg agcaggcgct ggcagcgcgc
     3001 ggcgcgctcg cacttccgca ggctctgcgt tacctggagc agatcgcgga ggcgctggat
     3061 tacgcgcaca cgcgcgggat tgttcaccgg gatgtgaagc ctgctaccat atttcttagc
     3121 gccgatgagc aggcgctggt agcggatttc ctcctgacct ccgcgctggc cgatgaagct
     3181 atcgctccga tgcgcctgtc atgtgccggg atgctggatt acatgtcgcc cgaactggtc
     3241 gtcggaaaag aagtagatgg gcgcgcggat atctactcgc tcggcgctct gctgtatcat
     3301 atggtaacag ggcaggcgcc cttccagggg cagacgctga tgaaggttgc cgccaaacac
     3361 ttgaaagcca ctccgccctc gccgagcgcg ctgcgtcccg atcttcctgt cgcggcagag
     3421 caagtgattt tgaaagcact ggaaaagcga cccgcgcatc gttatgacag cgcacatgac
     3481 ctcgcgcgct ccttccgccg ggccgtgctg ggagagcatg tacaggtggc gcaac
//
LOCUS       DASZAK010000627         3756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_621160, whole genome shotgun sequence.
ACCESSION   DASZAK010000627 DASZAK010000000
VERSION     DASZAK010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621160"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..414)
                     /locus_tag="VGT44_10990"
     CDS             complement(124..414)
                     /locus_tag="VGT44_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581366.1"
                     /translation="MMDLAELTWGRYNSNQVAPIGSYLNLRTLCVFQQTSDGPVPADG
                     TFHRVDPSGTQTFANIATTVNGVLGTAYTSGSFHACAGGDAATVPGAASDDA"
     gene            complement(566..1201)
                     /locus_tag="VGT44_10995"
     CDS             complement(566..1201)
                     /locus_tag="VGT44_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581367.1"
                     /translation="MGYIGNDGSALAGGLNPSSVVQGLAVDASGRLITTNYIGGAAVG
                     VGNPYPSQDQIRQWCINGNVFSASLEATTGQTNAGISLYIPANTPKNIMIVSARGSAA
                     GVGSLLQITTQATDFALGSTGLVGPVNQNFASAAASALTVSGSKSATVAGTQKDSAFC
                     SSASTEYDLLMAATKVIYYPPNQAGALAIGFWVVPATTNNNLSLVVTWVEW"
     gene            complement(1205..3307)
                     /locus_tag="VGT44_11000"
     CDS             complement(1205..3307)
                     /locus_tag="VGT44_11000"
                     /inference="COORDINATES: protein motif:HMM:NF021628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HEV2581368.1"
                     /translation="MQQLAITGMQQERMRVPGFIREGQALDRQGHEALVTIIRGGKSI
                     NGYYYAEAALQQIAQMIEGARAYVDHGRSEADQQTRSVRDMVGFYQDARYVPPGDGQA
                     HGRVDATLHILEAASWLWSMLCEAVALGKPNLIGLSIDIFGAWEQADSARNVTRIVSL
                     NSCDVVTRPSAGGAIKRVLHSDALAPVDQDNPPSSEPPQSTPSSQSLLQGESSFMDIQ
                     ETQLPQTPTASPPETPRPASQATTPASHISEAQQAVINAALADIQRERCALLLERRLL
                     ETQLPQAAQQQVRAQFTGRIFEETELESGLGAMRTLLTELHTAGLIKGNGYEKPQAGQ
                     MISEAEKIQAAFDRMFDLEIDTARLGNIRGFSSIREAYARVTGDGAVAGFSDRTTLGA
                     IQISEHAPVVRITEADTTTASFSYLLGTSMNKRLLRDYQAWPAEWQKFCTIAPIKDFK
                     QQVRVRLGAFGSLSTVAEDTAYSTISLSDTAATYVPSKRGNLVTVSRETIVNDDLFAI
                     KQIPTKLAVAAAYTLAEFVYSFLSANPTIYDGNALFTSGAPHSNLGSSALSTAAMQTG
                     VTAMREQTNFAGKRLGLRPTFLIVPPELEFTAMVVTKSAGVPGSNNNDINPMLGYVTP
                     IVSPQLSNTSQWFFASDPKVVDTIEIGFVGGQVNPALFIQDQPLFGLNFTQDVISYKV
                     RQEYGGAVMDWRGLYRGI"
     gene            complement(3314..>3756)
                     /locus_tag="VGT44_11005"
     CDS             complement(3314..>3756)
                     /locus_tag="VGT44_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581369.1"
                     /translation="PLLRPYDLAGPGLDAIAPLRPDPQVGDLLRYGQPLGLRVYPAGD
                     AAPDPALPDLDDYDRPAGLAMPGALAVDPPDLQSPMLAPQVTMPDRPADLSPGALDIM
                     HDSASYRQLGDTPYREVYMDASGMNASRRRHLDLLMRGLDEEEG"
BASE COUNT          654 a         1165 c         1211 g          726 t
ORIGIN      
        1 gccggttcac cgtgcgcatc gccgatttat cggcctgtgg agcgcgtcga cggattaccc
       61 tggccgataa atcggcggtg cgcacggtga accggcgcct acgggattcg caacgttttt
      121 cgatcaggcg tcgtcggacg cggctccggg aaccgtcgcg gcatcacccc cggcgcaggc
      181 gtggaagctg cccgacgtgt aggccgtccc aagcacgcca ttgacggtcg tggcgatgtt
      241 ggcgaaggtc tgcgtaccag acgggtcaac gcgatgaaac gtgccatccg ccggtaccgg
      301 cccatcactg gtctgctgga acacgcacag ggtacgcagg ttgaggtaac tgccgatagg
      361 ggcgacctgg ttgctgttgt agcggcccca ggtcagttct gctaaatcca tcatgtgaag
      421 gggtatcctt tctgtgttat gtctaactac caaaatgcaa tggggtgtag gggcgcaatt
      481 cattgcgccc tcgttcccgg tcatgcgctt tcaccagggc gcaatgaatt gcgcccctac
      541 accccattgc aaccttgacg gtccactacc actcgaccca ggtgacgacc aggctcaagt
      601 tgttgttggt ggtggccgga accacccaga agccgatagc gagcgcgcct gcctggttcg
      661 gcgggtaata aatcaccttg gtcgccgcca tgagcagatc gtactcggtc gaggccgagc
      721 tacaaaaggc cgaatctttc tgcgtccctg ccaccgttgc gcttttcgag ccggagacgg
      781 tcagcgcgct cgcggcggcg ctggcgaaat tctgattgac cggcccgacc aggccggttg
      841 aaccgagcgc gaagtcggtg gcctgcgtgg tgatctggag caggctgccg acacccgcgg
      901 cagagccgcg cgccgagacg atcatgatgt ttttgggcgt gttggccggg atgtagagcg
      961 agatgccagc gttcgtctgg cccgtcgtcg cttcgagcga agcactaaag acgttgccgt
     1021 tgatgcacca ctgccggatc tgatcctggc tcggataggg gttgccgacg cccactgccg
     1081 cgccgccgat gtagttggtg gtgataagcc gcccactcgc atcgaccgca agtccctgca
     1141 caacgctcga cgggttgaga ccgcccgcca gggcggagcc gtcgttgccg atataaccca
     1201 tgacttaaat ccctcgatag aggccgcgcc aatccatcac cgctcctccg tactcttgac
     1261 gcaccttgta ggagatgaca tcttgtgtaa aattcagccc aaacagcggc tgatcctgga
     1321 tgaagagcgc cgggttgacc tggccgccca cgaagccgat ctcaatggta tcaaccacct
     1381 tcgggtcgga agcgaagaac cactggctcg tattgctgag ctgcggcgag acaatcggcg
     1441 tgacatagcc cagcatcgga tttatgtcat tattattgct gccaggcacg cccgccgatt
     1501 tggtcacgac catggcggtg aactccagtt ccggcggcac aatcaggaag gtcgggcgca
     1561 gtcccaggcg tttgcccgcg aaattggtct gctcgcgcat ggccgtcacg cccgtttgca
     1621 tcgccgccgt cgagagcgcg gaggaaccga ggttgctgtg cggcgcgccg ctcgtgaaaa
     1681 gcgcgttgcc gtcgtagatg gtgggattgg cagagaggaa gctatagacg aattcggcca
     1741 gcgtatacgc ggcggcaacg gccagcttgg tcggaatttg cttgatggcg aacaggtcat
     1801 cattcacgat ggtctcgcgc gagactgtca ccaggttgcc gcgtttgctc ggcacgtagg
     1861 tcgcagccgt gtcggaaagc gagatggtgc tgtaagccgt atcttccgcc accgtcgaga
     1921 ggctgccgaa cgcgcccagg cgcacacgca cctgctgctt gaaatccttg atgggcgcga
     1981 ttgtgcagaa tttctgccac tccgccggcc acgcctggta gtctctcagc aggcgcttgt
     2041 tcatcgacgt gccaagcagg taggagaaac tggcagtggt cgtatcggcc tcggtgatac
     2101 gcacgactgg cgcgtgttcg ctgatctgga tcgctcccag cgttgtgcgg tcgctgaagc
     2161 ccgccaccgc accgtcgccc gtcacacgcg cgtaggcttc gcgaatgctg ctgaagccgc
     2221 gaatgttgcc gagccgggca gtatcgatct ccaggtcaaa catgcgatca aaggccgcct
     2281 ggattttctc cgcctcgcta atcatctgcc cggcctgggg cttttcgtag ccgttgccct
     2341 tgatgaggcc cgccgtgtgc agttccgtca gcagcgtgcg catcgcgccc agaccgctct
     2401 ccagctcggt ctcctcgaag atgcgcccgg tgaactgcgc gcgcacctgc tgctgcgcgg
     2461 cctgcggcaa ctgcgtttcc agcagccgcc gctccaacag cagcgcgcat cgctcgcgct
     2521 ggatgtcggc cagcgccgcg ttgatgacgg cctgctgcgc ctcgctgata tggctcgcgg
     2581 gggttgtcgc ctgggaagcg gggcgaggtg tctccggcgg ggatgcggtc ggagtttgag
     2641 gaagttgtgt ttcctggatg tccatgaatg aggattctcc ttgtaatagg gattgactgg
     2701 atggtgtgga ttgaggaggt tctgaggatg gtggattgtc ttgatcgacc ggcgcgagcg
     2761 cgtcggagtg caggacgcgc ttgatggccc ctccggcgct ggggcgcgtc accacgtcgc
     2821 acgaattgag ggagacgatg cgcgtgacat tcctggcgct gtcggcctgt tcccacgcgc
     2881 cgaagatgtc gatggagagg ccgatcaggt tcggcttacc cagcgcgacg gcctcgcaca
     2941 gcatcgacca gagccaggag gccgcttcca ggatatgcag cgtcgcgtca acccgcccat
     3001 gcgcctgccc gtcgcccggc ggcacatagc gcgcgtcctg gtagaagccc accatatcgc
     3061 gcaccgagcg cgtctgttga tcggcctcgc tgcgcccgtg atcgacatag gctctcgcgc
     3121 cctcgatcat ctgcgcgatt tgctgcaaag cagcctcggc gtaataatag ccgttgatgc
     3181 ttttgccgcc cctgatgatc gtcaccagcg cctcgtgtcc ctgccggtcg agcgcctgcc
     3241 cctcgcgaat gaatccgggg acgcgcatgc gctcctgctg catcccggtg atcgccaact
     3301 gctgcatggc cgcttatcct tcttcctcgt cgaggccgcg catcagcagg tcgagatgcc
     3361 gtctgcgcga ggcgttcatg ccagatgcgt ccatgtagac ctcgcgatac ggcgtatctc
     3421 ccaactgccg atagctggca ctgtcatgca tgatatccag cgcgccaggc gacaggtcag
     3481 cgggccggtc aggcatcgtg acctgcggcg caagcatggg agattgtagg tcgggcggat
     3541 cgaccgccag cgcgccaggc atcgccaggc ccgccgggcg gtcgtagtcg tccaggtcgg
     3601 gcagcgccgg gtcaggcgcg gcgtcgcctg ccgggtagac gcgcaggccg agcggctgcc
     3661 cataacgcag caggtcgccc acctgcggat cgggccgcag cggcgcaatc gcgtcgaggc
     3721 caggaccggc caggtcgtag ggacgaagca gaggca
//
LOCUS       DASZAK010000628         2823 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_335413, whole genome shotgun sequence.
ACCESSION   DASZAK010000628 DASZAK010000000
VERSION     DASZAK010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2823
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335413"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VGT44_11010"
     CDS             <1..858
                     /locus_tag="VGT44_11010"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV2581370.1"
                     /translation="GALAIAIADLPGFALLERGQRFSVQSRFAQQDEPDNARKRAYST
                     GMLNQQLAQAFAEETGAVEHIKKPQVVVSVLCTFEQAYTGISLARENLSDWPGGMRHF
                     AHTADEISRAEFKLLEALEVFGLALPSSGSALDLGAAPGGWTRLLLEAGLRVVAVDPA
                     QLDPRLYRHPRLEHYRGYAEGYLEEALRRRVKFDVIANDMRMDAREAARALVQAAPCL
                     QPGGIVISTLKLPHSTYEIDPLKNLNEALHVLNQRYDVTQARQLFHNRQEVTVVAARP
                     RSKPVYTGR"
     gene            861..2486
                     /locus_tag="VGT44_11015"
     CDS             861..2486
                     /locus_tag="VGT44_11015"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEV2581371.1"
                     /translation="MSSEARQSPGTVQPVLPPVIGPLRRHTHTYDLMRQCIHCGFCLP
                     TCPTYAVLGVEMDSPRGRIYQMQAVAEGRMEISPEFVEHMYCCLGCRACETACPSGVQ
                     FGKLIEAAREQIQIEVAHTPLFDHRGDGQSQSDVPALPEPEGNAINPLHLDEGRSPAL
                     ESLSERLLRRFFFDIMLPSRLVTSLVFGGLKVYQRSGLQALTHRSGLLDIVNELPTPF
                     QGQLKTPESLMPSARGDVLPKPIAEYTPARGKQRYRVGFVSGCIMDQVFRDINEATIR
                     VLAANDCEVVTPLQQQCCGALHVHAGEAERGRLLAQRNIDVFEPYACDYIIINSAGCG
                     SNLKEYGHLLRDDALYARRAEAFSAKVLDISEFLARVDLNREMGEVKRTVAYHDACHL
                     FHGQKVKQQPRDLLKAIPGLTIVPLKEAEWCCGSAGIYNITNQEMASQLLERKMNNIA
                     ATGVQVIATGNPGCMMQIALGARERGLDLQVMHPVELLDEAYRAADLYDLSIPEKISD
                     QGRQRSMLVWLGIGLGIGLLLAFRRNRKRKGTE"
     gene            complement(2679..>2823)
                     /locus_tag="VGT44_11020"
     rRNA            complement(2679..>2823)
                     /locus_tag="VGT44_11020"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          570 a          848 c          836 g          569 t
ORIGIN      
        1 ggcgcgctgg ccattgccat cgccgatctt cccggcttcg cgctgctcga acgcggccag
       61 cgcttctcgg tgcagtcgcg tttcgcgcag caggacgaac cggacaacgc gcgcaaacgc
      121 gcctactcca ccggaatgct caaccagcaa ttggcgcagg cattcgcgga agagactgga
      181 gccgtggaac atatcaaaaa gccgcaggtc gtcgtctcgg tgctttgtac cttcgagcaa
      241 gcctataccg gtatctcgct tgccagggag aatttgagcg attggccggg cggtatgcgc
      301 cacttcgcgc atactgcgga cgagatcagc cgggcggaat tcaagttgct ggaagcgttg
      361 gaggtgtttg gcctggcgct gccatccagc ggcagcgcgc ttgacctggg ggccgcgccc
      421 ggtggctgga cgcgcctgct tttagaagcc ggtctgcgcg tggtggctgt tgacccggcg
      481 cagcttgacc cgcgtctcta tcgccatccc aggctggagc attatcgcgg ctatgccgag
      541 ggatacctgg aagaggcgct gcgacggcgc gtcaagttcg atgtgatcgc caacgacatg
      601 cgcatggatg cgcgcgaggc ggcgcgggcg ctggtccagg cagcgccatg cctgcaaccc
      661 ggtgggatcg tcatcagcac gctcaagctg ccacattcga cttatgagat cgatcccttg
      721 aagaatctga atgaggcgct tcatgtgctc aaccagcgtt acgatgtgac gcaggccagg
      781 cagttgtttc ataatcgcca ggaagttacc gttgtcgccg cgcgaccgcg cagcaagcca
      841 gtctacaccg ggaggtagcc atgtcatctg aagctcgtca atcaccgggc accgtacagc
      901 cggttctgcc gcccgtcatc gggccgctgc gccgccatac acatacctac gacctgatgc
      961 gccagtgcat tcactgcgga ttctgcctgc ctacctgccc aacctatgcc gtgctgggcg
     1021 tcgagatgga ttctccacgc gggcgtatct accagatgca agccgttgct gagggtcgca
     1081 tggaaatctc gcccgaattt gtcgaacaca tgtattgctg cctgggctgc cgggcctgcg
     1141 aaacggcctg cccttccggc gtacagttcg gtaaattgat cgaagccgcc cgcgagcaaa
     1201 tccagataga ggtggcgcat accccacttt tcgatcatcg cggagatggt cagagccaat
     1261 ccgatgtacc cgcgttaccg gagccggagg gcaacgccat taacccgctt cacctggacg
     1321 agggtcgctc gccggcgctc gaatcgctca gcgagcggct actgcgacgt ttctttttcg
     1381 atatcatgct gccctctcgc ctggtgacat cgttggtttt cggcggcttg aaagtatacc
     1441 agcgcagcgg cctgcaggcg ttgacacatc gcagcggcct gctggatatc gtcaatgaac
     1501 tgcccacgcc gtttcagggg caactgaaaa cgccagagtc gctgatgccc tccgcgcgcg
     1561 gcgatgtgct gcccaaacct atcgccgagt atacaccggc caggggcaag cagcgctacc
     1621 gcgtcggctt cgtcagcggc tgtatcatgg accaggtgtt tcgcgacatc aacgaggcga
     1681 ctattcgcgt gttggcagcc aatgattgcg aggtggtgac accgctacaa cagcaatgct
     1741 gcggcgcgct gcatgtccac gccggtgaag ccgagcgcgg gcggttgctt gctcagcgca
     1801 acatcgacgt gttcgagccg tatgcctgcg attacatcat catcaattca gccggttgtg
     1861 gctccaacct gaaagagtac ggccacctgc tgcgcgacga tgccttatat gccagacgcg
     1921 ccgaagcatt tagcgcgaag gtactcgaca tcagcgagtt tttggcgcgc gtcgatttga
     1981 accgcgagat gggtgaggtc aaacgcacgg tcgcctatca cgatgcttgc catctcttcc
     2041 acgggcaaaa ggtcaaacag cagccccgcg accttctgaa agctattcct ggcctgacga
     2101 tcgtacccct caaagaggcg gagtggtgct gcggcagcgc gggtatttac aacatcacga
     2161 accaggagat ggcatctcaa ttgctcgaac gtaaaatgaa caatatcgcc gcgacgggcg
     2221 tgcaggtgat cgcgaccggc aaccccggct gtatgatgca gattgcgctg ggcgcgcgcg
     2281 aacgcggcct ggatctccag gtcatgcacc ctgtagaatt gctcgatgag gcatatcgcg
     2341 ccgccgatct ctacgacctt tccatccctg aaaagatcag cgatcaaggc cgccagcgct
     2401 cgatgctggt ctggctgggt attggcctgg gtatcggcct gctactggcg ttccgccgga
     2461 ataggaaacg caaaggaaca gaataggaca caagcaaagc agcccgcttc cattgacgga
     2521 agcgggctgc tttgcttgtg tcctataatt cttacttctg cgcagctatt acaattacct
     2581 caattttgcc gagaccagcc gctcatcaaa caattgagag caacagagcc agcgaatgag
     2641 caaccatgtg aagaatatcc aggttatgaa tgtgagttgt aaacaagccg ttgggatatg
     2701 agccccgctt ggctgcgccg gttacccggc gtccacccgc ggtctcgcga ccagctgttc
     2761 tggctggatc cttcatcgtt gctgccaacg atgggaaacc tcctcttggg ggcggcttcg
     2821 cgc
//
LOCUS       DASZAK010000629         6249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_656057, whole genome shotgun sequence.
ACCESSION   DASZAK010000629 DASZAK010000000
VERSION     DASZAK010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6249
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656057"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VGT44_11025"
     CDS             <1..263
                     /locus_tag="VGT44_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEV2581372.1"
                     /translation="YRGPIQEITQQGDTYILRLPLPHVEMDKVLMTKKGDEMIVEIGN
                     FKRDITLPNVLANQEAKLARFVNKALEIHFTAPAEEEVEKSA"
     gene            complement(268..1257)
                     /gene="add"
                     /locus_tag="VGT44_11030"
     CDS             complement(268..1257)
                     /gene="add"
                     /locus_tag="VGT44_11030"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEV2581373.1"
                     /translation="MDYTALPKVELHLHLDCSLSYEAVARIDPSVTLREYQQSYNGPE
                     KYSDLSEFFGRAPKGTALMQTEEQLRLVTGDVFKQLELDHVIYAEIVLVPTMHTGQGL
                     TPDEVVEIVDDAVARESEASGIEARLILSTLRHYSPQQSMATVKQVERFMGRHVGGID
                     LGAYEAGYPIYAHIAAYQYAAQQGIPRTVHAGEARGPDSVWETLKHLRPSRIGHGVRS
                     IEDPALVAHLRQERIHLEICPSSNIQIDLFEDYAHHPINVFYEEGLSIGVNTDTRMIA
                     NVTLAQEYEKLNRAFGWDERHFLRCNLDALAAAFAPDALKQPLEHRLRAGYEG"
     gene            complement(1394..1858)
                     /locus_tag="VGT44_11035"
     CDS             complement(1394..1858)
                     /locus_tag="VGT44_11035"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEV2581374.1"
                     /translation="MKWITREHVKVDRVACPWLIKKFVDKDAEFLFVPTEQVAEAASR
                     EGAIPYDVKNVELGHHGKECSFEAILKKYNLTSDAALVLLGRIVNGADTDNTLWNQPE
                     GPGLEAIAEGFRHLGFKDDHEQNAAEWIVYDALYAYCQEMVRQGKPNGDFVS"
     gene            1984..3399
                     /locus_tag="VGT44_11040"
     CDS             1984..3399
                     /locus_tag="VGT44_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2581375.1"
                     /translation="MISSHMFFCPECGAANSNDAGICFVCHEPLTHPDEQELSPLATS
                     SSMAQASGQAYQVQLISAALPVLTGPLLPGSLLKERYRIIGEIGQGGYGIVYKATDTQ
                     KRNRLVAVKQIDLSALTTRQVIEVTDSFNREVQILSKLRHKNLPRIYAHFTDPQHWYV
                     VMDYIEGQTLEDYLARKQNGRLSAQEAARIGIQLTNVLDYLHSQQPPIIFRDVKPANI
                     MRTPRGRLYLIDFGIARLFNPQKTRDTGPLGSPGFAAPEQYGRAQSTAQTDIYGLGAT
                     LQSLLQGSETTEDESANQAASETPGEMAHTTPGSTATTAIPPSLRKLLNQMLEYDATR
                     RPKHMQEVRRRLELLPVNIPLMLLNIFRRSFWGLVIGSIPYSFAFLLLLVSSVTIIDQ
                     AISWFVFVITLLSFSLWYIVLGLQLILAFTALHSKRHRLMGIGILVMLAVIFLAALFG
                     WLPWPWNPSGRPFPFGYGEYL"
     gene            3474..>6249
                     /gene="recG"
                     /locus_tag="VGT44_11045"
     CDS             3474..>6249
                     /gene="recG"
                     /locus_tag="VGT44_11045"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV2581376.1"
                     /translation="MSNTTQSLLAPYIERARKVLQYEQRGNHQDKVVQGGLELFVVRW
                     ADEVSAACKSASLDVKPLYHFTEHLEGYRRQDPMQRAASLRAALAILNELEDDTPVAI
                     TGKAHINVVSGKPGRSEAPGRPRGSSLPYPNDDPVQGRGNPRGRPGITADIGSPAGRP
                     GGITADIDTPPNAVSNTNVGTPNGQGVQPKQTGDASPGKIDQRERVHLDAGMTANHAS
                     LALLSADVTAVPGVGPSVAARLRGLGIRTVRDLLFYFPRQHRDYSKLEKIVNIPFEQV
                     TTTLGLIWEVETTHTSGGRTRTIATISDDSGKLRVSWFNQPYMQKQLQAAKGEYLVVT
                     GTKRRFGNKVEFNVQSYELPERGDLLSTGRLVPIYSLTEGLHAKTLRRFTKWAVDRYA
                     SMIPEHLPASLRSAAKLLPLPEAVSQIHYPENETMLQAARRRLGFDELFLIHLGMQER
                     RTRWQREAPRGNAFHIDYKKIFADTSNLLALPASQDETVPAQTETTDTMGATLWSSMA
                     TDQPFETTLPFRFTEAQRRVIVEICGDLAKSQPMGRLLQGDVGAGKTAVAAAALLLAA
                     LNGYQGAIMAPTELLAEQHARSISTMLEPFGIRTVLMTGSLRSKERAMGRAAIENGQA
                     MVAIGTHALIQEDVAFQKLGLVIVDEQHRFGVEQRDILRQKGYHPHMLVMTATPIPRT
                     LALTLYGDLDVSVIDQLPPGRQKIISRWRTGARRDEANALIEQEAQEGRQAFIICPLI
                     EDSETLAVKSATAEYERLSRDVFPHLRLGLLHGSLKAAEKDATMRRFRDGELDILVAT
                     SVIEVGIDIPNATVMVIEDADRFGLSQLHQFRGRVGRGQHQSYCYVLSADASITAQER
                     LDVFQRTDDGFRLAEADMKLRGPGDFFGVRQSGTPELRIADLTDTKLIEQSRELATRL
                     WEQDPYLKKPE"
BASE COUNT         1339 a         2035 c         1667 g         1208 t
ORIGIN      
        1 tttatcgcgg ccctatccag gaaatcacac agcagggtga cacgtatatc ctgcgcctgc
       61 ccctgccgca cgtcgagatg gacaaagtgc tgatgaccaa aaaaggcgat gagatgatcg
      121 tcgagatcgg caacttcaaa cgcgacatca ccctgccgaa cgtgctggca aaccaggaag
      181 ccaaactggc ccgcttcgtc aataaagccc tcgaaatcca ctttaccgcc cccgccgaag
      241 aagaggtgga gaagtcagcc tgattcgcta tccctcgtat cccgcgcgca gccgatgctc
      301 aagcggctgc ttgagggcat ccggcgcgaa ggcagcggcc agcgcatcca ggttgcaccg
      361 caggaagtgg cgttcatccc aaccgaaggc gcggttcagc ttttcgtact cctgcgccag
      421 cgtcacgttg gcaatcatgc gcgtatccgt attcacgccg atcgagagac cttcttcgta
      481 gaacacattg atgggatgat gcgcgtagtc ctcgaacaga tcgatctgga tgttggacga
      541 agggcaaatt tcgagatgga tgcgctcctg cctgaggtgc gccaccagcg ccgggtcttc
      601 gatactgcgc acaccatgcc cgatacgcga cgggcgcagg tgcttgagcg tctcccacac
      661 actgtccggg ccacgcgcct cgcccgcgtg aacggtgcgc ggaatgccct gctgcgcggc
      721 atactggtag gcggcaatgt gcgcgtagat gggatagccc gcctcgtatg cgcccaggtc
      781 gatgccgccg acgtgcctgc ccatgaagcg ttcgacttgc ttcaccgttg ccatgctttg
      841 ctgcggcgag tagtggcgca acgtgctaag gatcagccgc gcctcaatac cgctcgcctc
      901 gctctccctc gccaccgcgt catcgacaat ctccaccacc tcgtccggcg tcagcccctg
      961 gccagtatgc atagtaggaa ccagcacaat ctccgcgtag atgacatgat ccagctcaag
     1021 ctgcttgaag acatcgccgg taacgaggcg caactgctct tccgtctgca tcaaagccgt
     1081 gcctttcggg gcgcgcccaa agaactccga caaatcgcta tatttttccg gcccattata
     1141 cgattgttga tactcgcgca acgtaacgct cggatcgatg cgcgctacag cttcatagct
     1201 cagcgagcaa tcgaggtgca ggtgcagttc caccttcggc agcgctgtat aatccatccg
     1261 tatgtattcc ccctactcga ttttcaccgg ctatcgtatc attcatgtca accttgacag
     1321 acctgtccga tcacatcggt gccaaccacg tcgccgattc atccccctcg ttccgcgtca
     1381 ggtgtgcctc gattcacgag acaaagtcgc cgttgggctt gccctggcgc accatttcct
     1441 ggcaataggc atagagggcg tcgtacacga tccactcagc ggcgttctgc tcgtggtcgt
     1501 ccttgaaacc gagatggcgg aagccctcag cgatggcctc caatccaggt ccctcgggct
     1561 ggttccagag cgtattgtcg gtatccgcac cgttgacgat cctgcccagc aggaccagag
     1621 ccgcatcgct cgtcaggtta tacttcttga ggatggcctc aaacgaacac tccttgccgt
     1681 gatggcccag ttccacattt ttcacgtcat agggaatcgc cccttctcgg gaagccgcct
     1741 ccgccacctg ctcggttggg acgaacagga actcagcgtc cttgtccaca aactttttga
     1801 tgagccaggg acaggccacc cggtctacct tgacgtgttc acgagtgatc cacttcattg
     1861 catgtttctc cttcctggtg agttgaattg catcgaatgc cattactatt ctatcagaga
     1921 ggaaaccatc cttttcggcg taatagtata ttgtaagcct gatgaaagaa agcatacgcc
     1981 aatatgatct catcacatat gttcttttgc ccggaatgcg gcgcggccaa ctcgaatgat
     2041 gcaggcatat gtttcgtttg ccacgagccg ctgacccacc cggacgagca agaattatcc
     2101 ccgctagcta cttcatcatc gatggcacag gcatccggtc aagcctacca ggtgcaactt
     2161 atcagcgctg ccctgccggt gctgaccggg ccgctgctgc ccggctcgct gctcaaggaa
     2221 cgctatcgca tcatcggcga aataggccag ggaggctatg gcatcgtcta caaggccacc
     2281 gacacacaga aacgtaacag gctggtggcc gtcaagcaga tcgacctgag cgccttgacg
     2341 acccgccagg tcatcgaggt cacggattcg ttcaaccgcg aggtgcagat actctcgaaa
     2401 ctgcgtcata agaatctgcc gcgcatctac gcgcatttca ccgacccgca gcactggtac
     2461 gtcgtgatgg attatatcga agggcagacg ctcgaagatt acctcgcccg caagcaaaac
     2521 gggcgtctca gcgctcagga agcagcacgc atcggcatcc agctcacaaa cgtgctggac
     2581 tacctccatt cgcagcagcc gcccattatc ttccgcgatg tcaaaccggc caacatcatg
     2641 cgcacaccgc gcgggcggct ctacctgatc gacttcggca ttgcgcgcct cttcaacccg
     2701 cagaaaacgc gcgataccgg cccgctcggc tcgcccggct tcgccgcgcc cgaacaatac
     2761 ggcagggcgc aatcgaccgc ccagaccgac atctacggcc tgggagccac attgcagtcg
     2821 ctattacagg gttccgaaac cacagaagat gagtcggcaa accaggcggc cagtgagaca
     2881 ccgggtgaga tggcccatac tacgcctggc tcgacagcca caacagcaat ccctccatcg
     2941 ttgcgaaaac tcttaaacca gatgcttgag tacgatgcga ccaggcgccc aaagcacatg
     3001 caggaggtgc ggcgtcgcct ggaattactg cccgtcaaca taccgctcat gctcctcaac
     3061 atctttcgcc gttcattctg gggactggtg atcggctcca ttccctattc cttcgcattc
     3121 ctgctcttgc tggtctcgtc tgtcaccatc atcgaccagg caattagctg gtttgtcttc
     3181 gttattacct tactctcctt cagtctgtgg tatatcgtgc tggggctgca acttatcctc
     3241 gccttcacag cgctgcattc gaagcgtcac cgtctcatgg gcataggtat tctggtcatg
     3301 ctggcagtaa ttttcctggc ggctttgttc ggatggctgc cctggccctg gaacccttct
     3361 ggccggccat ttcctttcgg ctacggcgaa tatctctgat cttgacacat ctgcaaaaca
     3421 cccactatag tatgggggct gaatgaattc catcttgcag tatgaaataa cctatgtcaa
     3481 acacaacaca gagcctgctg gcgccctata tcgaacgcgc ccgcaaagtc ctgcaatacg
     3541 agcagcgcgg caatcaccag gataaagtcg tccagggagg actggaatta tttgtggtgc
     3601 gctgggccga cgaggtcagc gcggcctgca aaagcgccag tctcgacgta aagcccctct
     3661 accacttcac cgaacacctg gaaggctacc gccgccagga ccccatgcag cgcgccgcca
     3721 gcctgcgcgc cgcactcgcc atcctcaacg aactcgaaga cgacacacct gtcgcaatca
     3781 caggaaaagc gcatatcaac gtcgtgtcag gaaaaccagg gcggtcggaa gcaccaggac
     3841 gaccgcgagg gtcgtcccta ccctatccga acgacgatcc cgtacagggt aggggcaacc
     3901 ctcgcggtcg ccctggtatc accgccgaca tcggttctcc cgccggtcgc cctggtggta
     3961 tcaccgccga catcgatacc ccacccaacg ccgtctctaa caccaacgtc ggcactccca
     4021 acggacaggg tgtccaacca aaacaaaccg gcgatgcctc gccgggtaaa attgaccagc
     4081 gtgagcgtgt ccacctcgat gcgggcatga cggccaacca tgcatcgctg gcgctgctca
     4141 gcgcggatgt cacagctgta ccgggcgtag gaccgtcggt cgcggcgagg ctgcgcggac
     4201 tgggcatccg cacggtgcgc gacctgctct tctatttccc gcgccagcat cgcgattaca
     4261 gcaagctcga aaaaattgtc aatatcccct tcgagcaagt caccaccacg ctgggcctca
     4321 tctgggaggt cgagacgacg cacacgagcg gcgggcgcac acgcacgatt gctaccatct
     4381 ccgacgatag cggcaagttg cgcgtctcct ggttcaacca gccctatatg cagaagcagt
     4441 tgcaggccgc caaaggcgaa tatctcgtcg tcacgggtac aaagcggcgc ttcggcaata
     4501 aggtggaatt taacgtgcag agctacgaat tgcccgaacg cggcgatctg ctcagtaccg
     4561 gtcggctcgt cccgatttac tccctgacgg aagggctgca cgccaaaacg ctgcgtcgct
     4621 ttaccaagtg ggccgttgat cgctacgcct ccatgattcc tgaacatctc cccgcctcgc
     4681 tgcgatccgc cgctaaattg ctgcccctgc ccgaagcggt ttcgcagata cactatcccg
     4741 aaaacgagac gatgttgcag gccgcgcgcc gccgcctggg ttttgacgaa ctcttcttga
     4801 tccacctcgg tatgcaggaa cgccgcacac gctggcagcg cgaagcgccg cgcggcaacg
     4861 ccttccacat cgactataag aaaatttttg ccgatacgtc caatcttctc gccctccccg
     4921 cttcgcagga cgaaacggtc ccagctcaga cagaaacaac ggatactatg ggcgcaaccc
     4981 tttggtcaag catggctacc gatcagccat tcgagacgac actgccgttt cgcttcaccg
     5041 aggcgcagcg ccgcgtgatt gtcgagatat gtggcgacct ggccaaaagc cagccgatgg
     5101 gccgcctgct acagggtgat gtcggcgcgg gcaaaaccgc tgtcgcggca gcagcgctct
     5161 tgctggccgc gctcaacggc taccagggcg cgatcatggc ccctaccgaa ctgctggccg
     5221 agcagcacgc gcgcagcatc agcaccatgc tcgaaccgtt cggcatccgc accgtcctca
     5281 tgaccggcag cctgcgctcg aaagaacgcg ccatgggccg cgccgccatc gaaaatggac
     5341 aggccatggt tgccatcggc acacacgccc tgatccagga agatgtcgcc tttcagaaat
     5401 tggggctggt catcgtggac gagcagcacc gcttcggcgt cgaacagcgc gacatcctgc
     5461 gacagaaagg ctaccacccg cacatgctcg tcatgacggc cacgcccatc ccgcgcacac
     5521 tggccctcac gctctatggc gacctggatg tctcagtaat cgaccagctc ccgcccggtc
     5581 gtcagaagat catctcgcgc tggcgcaccg gcgcgcgccg cgacgaggcc aacgcgctga
     5641 tcgaacaaga agcgcaagag ggcaggcaag ccttcattat ctgtcccttg atcgaggatt
     5701 cagaaacact ggccgtcaag tccgcaacgg cggaatacga gcgactcagt cgcgacgtgt
     5761 tcccgcacct gcgcctggga ctgctgcacg gcagcttaaa agcagccgaa aaggatgcga
     5821 caatgcgccg ttttcgcgat ggcgagctgg atatcctggt cgccacctcc gtgatcgagg
     5881 tcggcatcga tatccccaac gcgacggtca tggtgatcga ggacgccgac cgtttcggcc
     5941 tctcgcaact gcatcagttt cgcgggcgtg tcgggcgcgg ccagcaccag tcatattgct
     6001 acgtcctcag cgccgacgcc agcatcaccg cccaggaacg cctggatgtc ttccagcgca
     6061 ccgatgacgg cttccgcctg gctgaggccg acatgaagct gcgcggccct ggcgacttct
     6121 tcggtgtgcg ccagagcgga acgcccgaat tgcgcatcgc cgacctgacc gacacaaaac
     6181 tgatcgagca atcacgcgag cttgccaccc gcctgtggga gcaagacccc tatttgaaaa
     6241 agccggagc
//
LOCUS       DASZAK010000630         3602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_122083, whole genome shotgun sequence.
ACCESSION   DASZAK010000630 DASZAK010000000
VERSION     DASZAK010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122083"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1189)
                     /locus_tag="VGT44_11050"
     CDS             complement(56..1189)
                     /locus_tag="VGT44_11050"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV2581377.1"
                     /translation="MRLLAIIFGLALIVLVALDAFETIVLPRRVTRRIRLAVIFYRTT
                     RFGWTSLAHLIRSSARRDAFLGYIGPLSLLALLLFWAVLFVFGFGFVMWGLALPLNVA
                     DRPISFLTDLYLSGTTFFTLGLGDVTPVPGVGRFLVVSEVAFGFIFLALVISYVPVLY
                     QAFSRRELRISLLDARAGSPPTAVELLRRNGAGKETEELRTLLHEWEVWCADILESHL
                     SYPILAFYRSQHEQQSWVETLTVILDTCALILTGIDGTPIKAARFTFAAARHAVVDLA
                     QVLNAPPRAGVNRLSAAELTQVRYRLAAADIHLQEGPASEQKLAELRATYEPFVSGLA
                     RRIQVSLPPWLPPADALDDWQTSAWDEQFPSMHQTLDKVMHPE"
     gene            complement(1252..1839)
                     /locus_tag="VGT44_11055"
     CDS             complement(1252..1839)
                     /locus_tag="VGT44_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV2581378.1"
                     /translation="MTRADIDRLLAIEGRPGKLDGRGLNLKGIDLAYLDLEGALLNGA
                     DLSGADLREADLTRADLAGANLSGATLDGTIFRQANLHQAILSGATLREADLSEANVS
                     DAILCGANLSEATLNQADLSHTDLSGADLSGADLGIVRLFGAMLRGANLKGANLSRAH
                     LSRANFFGANLSQVELADTDLSEADFSGALLGENG"
     gene            complement(1887..2069)
                     /locus_tag="VGT44_11060"
     CDS             complement(1887..2069)
                     /locus_tag="VGT44_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581379.1"
                     /translation="MESLAFSFPLKADKTEEWRAWIGEILGPRRSQYEVFVRSVHDAH
                     ASHNQGEAFDSRKPGA"
     gene            complement(2576..>3602)
                     /locus_tag="VGT44_11065"
     CDS             complement(2576..>3602)
                     /locus_tag="VGT44_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581380.1"
                     /translation="RWGRYKAFPIAGTAVMTLGVFLLSRLNEHTSVLVESLSMAILGL
                     GVGLVTQVLVMAVQNAVEYRNLGAATSGVIFFRSIGGAFGTAIFGAIFSNQLAAHLAS
                     LAASLPPGVNLSAAENVAAIHHLPTTVRVEVVHAYAQSLSAVFLVTVPSVAVAFMLSW
                     LLPEVRLRTTTQATDVGHTFAMPTTRTSQQEIERALHVLTSRESQDQLYRRLAARVGV
                     DLDPLPTWLLFRLQEQAPISEEHLEKRLRVPADRLTPALEALRENGLVTTTAQVKGQA
                     GGELQLTPAGQGTLRTLTTACHDSLGELLDGWSPEQEAELTALLRRVTTTLLSEENKK
                     ELISPPA"
BASE COUNT          743 a         1115 c         1051 g          693 t
ORIGIN      
        1 atcctctcgt gtcctctgct ggcaatcgcc cgccatgctc acgaacctgt ctcgttcact
       61 cggggtgcat caccttgtcg agcgtctgat gcatagaggg aaactgctca tcccaggcac
      121 tggtctgcca atcatcgagc gcgtctgctg gcgggagcca gggcggcaga gagacctgga
      181 tgcgcctggc cagcccgctc acaaacggct catacgtcgc tcgtagctca gccagcttct
      241 gctcgctggc cggtccttcc tgcaagtgga tgtccgctgc cgccagccga tagcgcacct
      301 gggtcaattc ggcggctgac agccggttca cgccagccct gggaggcgca ttgagcacct
      361 gggccaggtc gacgaccgca tgccgggccg ccgcaaaggt aaatctggct gcttttatgg
      421 gcgtgccgtc gatgcccgtc agaatgagcg cgcaggtatc caggatgaca gtcagggttt
      481 ccacccagga ttgttgctcg tgctgcgaac ggtagaaggc caggatggga taggagaggt
      541 ggctctccag gatatcagca caccacactt cccactcatg cagcagtgtg cgcagttcct
      601 cagtctcctt gccagctcca ttgcgccgca gcagttcgac tgccgtcggt ggggaacccg
      661 cccgtgcgtc cagcaaggag atgcgcagct cccgccgcga gaaggcctgg tagagcacgg
      721 gcacatagct gatcaccagg gccagaaaga tgaagccgaa ggccacctcg ctgaccacca
      781 ggaagcgccc tacgcccggg accggggtga catcgcccag gccgagcgtg aaaaacgtgg
      841 tgccgctcaa gtagagatcg gtcaggaagc tgatcggccg gtcagcgacg ttcaggggaa
      901 gcgccaggcc ccacatgacg aacccaaagc cgaagacgaa gagcaccgcc cagaacaaaa
      961 gcagagcgag caaggacagt ggaccgatat agccgaggaa ggcatctcgc ctcgcgctcg
     1021 atcgtatcag gtgagcaagg gaggtccacc cgaatcgagt cgttcgatag aagatgacgg
     1081 ccaggcggat gcgccgggtc acgcgccgcg gtaagacaat cgtctcaaaa gcgtccaggg
     1141 cgaccagcac aatcagtgcc agtccaaaga tgatggcaag aagtctcatc tcaatcagct
     1201 cctatgtcag gtcttgtcta tcgcgcttct ccttccgttc ggggccgggc atcaaccatt
     1261 ctcacccagg agagccccac tgaaatctgc ctcgctcaga tcagtgtctg ccagttcgac
     1321 ctggctgaga ttggctccaa agaaattggc ccggctcaga tgagcccgac tgagattggc
     1381 ccctttgaga ttggctcctc tcagcatcgc accgaagagg cgaacgatgc ccagatcggc
     1441 gccactcaga tcagcgccac tcagatcagt gtggcttagg tcggcctggt tgagggtggc
     1501 ctcactgagg tttgctccac agagaatggc atcactcaca ttggcctcgc tcaagtcggc
     1561 ctcacgcagg gtcgctccgc tcaggatagc ttgatgcaga ttcgcctgtc tgaagatggt
     1621 cccatccagg gtcgccccgc tcagattcgc cccggccagg tccgctctgg tcaggtcggc
     1681 ttccctcaaa tcagccccgc tcagatcagc cccgttgagc agagcccctt ccagatcgag
     1741 atatgccaga tcgatgcctt tgagattcag tccacgtcca tctagctttc ccggccttcc
     1801 ttctatcgca aggagtcgat cgatatctgc ccttgtcaaa ggaggaaggg atcctccagt
     1861 tgcatttgat ctttcagttt ccatgctcat gctcccggct tgcgactatc gaatgcctcc
     1921 ccctgattgt gcgaggcgtg ggcatcatgg actgatctca cgaatacctc gtactggctg
     1981 cggcgcggtc ccaggatctc tccgatccag gctctccatt cctccgtctt atcggccttc
     2041 aggggaaagc taaaagctag tgattccatg ctgtttactt ccttgtctcg cttgctccct
     2101 gacccactcg ccctccaggg gataacccgc ccgctcctac tcctgtagac ccctgcaagg
     2161 tgcagcacgt gggcatagcc gttgcgctcc agcagttggc cgagggcctg gctagcaata
     2221 cagttctcat cggaacagta tacgacaatc gcgtcctctt tgcgaagtga ctgcagcgcc
     2281 gtccggaagg tagcaaaaaa agatggagcc aggaagatgc tgtcaatagc gacctaaaat
     2341 tccccagaag tgccgacgaa aaattcctta gatcatctgt tcaagagggc caactgcctt
     2401 agcatcgccc gcttttctcc tgggaatcca ggagaaaagc ggatctaacc ttgaaggggg
     2461 cagcgtagga cgaacgcgac gagcgcttca tcgctaccca gtcggccgcc ttacgctggt
     2521 gctgatgggc tcgctgatcg gattaaggag tatcagccag tgaaagctca acagatcaag
     2581 cgggagggct gatcaattct ttcttgttct cttcactgag cagggttgtc gtcacccgac
     2641 gaagcagagc ggtcagctcc gcttcctgtt cgggggacca gccatccagc agttcgccca
     2701 ggctatcatg gcaggcggtc gttagggtcc tgagcgtccc ctgtccggca ggtgtcagtt
     2761 ggagttctcc acctgcttga cccttcactt gcgctgtcgt tgtgaccagt ccgttctccc
     2821 gcagcgcttc aagggcaggt gtgagcctgt cggctggcac acgcaagcgc ttctccaggt
     2881 gttcctccga aatgggagcc tgctcctgga gacggaacag gagccaggta gggagaggat
     2941 cgaggtcaac gccaacgcgg gctgccagcc gacggtagag ttggtcctgg ctctcccggc
     3001 tggtcagcac gtgaagtgcc cgctcgatct cctgctggga agtgcgagtg gttggcatag
     3061 cgaaggtgtg gcccacgtcg gtcgcctgag tggtggtgcg cagccgcact tccggcagca
     3121 gccaggacaa catgaaagcc actgcaacac ttggcaccgt caccagaaag accgctgaaa
     3181 gcgattgggc gtaggcatgc accacctcca cacgcacggt cgttgggaga tggtggatcg
     3241 cggccacatt ctccgccgca ctcagattga ccccaggtgg cagcgaggca gccagggaag
     3301 cgagatgggc tgccagttgg ttggagaaga tggccccaaa gatagccgtg ccgaacgcgc
     3361 cgccaatcga gcggaagaag atcaccccag aggtcgccgc gcccaggttg cggtactcca
     3421 cggcgttctg cacggccatc accagcacct gcgtcaccag ccccactccc agtcccagga
     3481 tcgccatcga cagcgattcc accagcacgc tcgtgtgctc gttcaggcgt gagagcagaa
     3541 acacgcccag cgtcatcacg gctgtgccgg caattgggaa ggctttgtag cgcccccagc
     3601 ga
//
LOCUS       DASZAK010000631         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_265487, whole genome shotgun sequence.
ACCESSION   DASZAK010000631 DASZAK010000000
VERSION     DASZAK010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265487"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..1377)
                     /gene="chrA"
                     /locus_tag="VGT44_11070"
     CDS             complement(127..1377)
                     /gene="chrA"
                     /locus_tag="VGT44_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEV2581381.1"
                     /translation="MQTQENTLDSVQATQRGSVLEVLGVFTRLGLTSFGGPVAHLGYF
                     REEIVMRRKWVDERAYGDLVGLCQFLPGPASSQTGISLGIMRAGLPGGLAAWIGFTLP
                     SAILMTIFAYTTSVLHVSTSTGWLHGLMVVAVAVVAQAVWGMARNLAPDRPRATMAIL
                     ATIATLLWASGIGQVVIIVVAGLIGWLFLRKTGETTITSSSSLATPAQHIPIPRALAI
                     ISLALFFVLLLALPLLSLVVPGHTLALFASFYRSGALVFGGGHVVLPLLQSAVVAPSW
                     VTNGQFLSGYGAAQAVPGPLFSFSAYLGAVENPTPNGWLGAIIALLGIYLPSFLLLIG
                     TLPFWNALRKQPPFQAALRGINAAVVGLLLAALYTPVWTSTIKAPIDFLFALAAFGLL
                     VVWKCPPWLVVLLAALVGAGLSLL"
     gene            complement(1429..2829)
                     /locus_tag="VGT44_11075"
     CDS             complement(1429..2829)
                     /locus_tag="VGT44_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEV2581382.1"
                     /translation="MRSPGALLLLSCYELGHQPLHLATLTALLRRAGYAPTVVDTAVE
                     PLADDTIIQARFVGIAVPMHTALRLGELLARRIRLLNPSAHICFYGLYATLNEDYLLR
                     ELIDSAIGGEYEQPLLELVAALENSPDGTTSAIPGVSTRQQLSNPRIVRAPLVMPERA
                     GLPPLDRYARLEWNGETRIVGYAETTRGCKHTCQHCPITPVYQGRFFATPADRVLADI
                     RAQIQQGAQHITFGDPDFLNGPTHALRTTHALHQEFPEITFDATIKIEHLLQHRHLLP
                     EFKQNGCIFVVSAVESLNDEVLARLEKGHTSADVADAFELMEQVGIALRPSLMPFSPW
                     ETLASYIRMLNFIEERHLIEHIDPVHLSIRLLIPPGSALLNAPDRREWLRELAAAAYT
                     YRWQHPDPHMDMLHSQICALVEEAQRKGAGPIETFFHIKALALAMQGQDMCVSCGIGG
                     YGVRKVIPHLTETWFC"
     gene            complement(2826..3155)
                     /locus_tag="VGT44_11080"
     CDS             complement(2826..3155)
                     /locus_tag="VGT44_11080"
                     /inference="COORDINATES: protein motif:HMM:NF003817.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEV2581383.1"
                     /translation="MARIVQCVKLGRSLPGLDKPPFPGELGQRIYENVSKQAWDMWPA
                     QSTLIINHYGLNMADPEARKILREQMEEFFFGEQPKMPEGWTPEEDEGVGVGPSTKGG
                     GAPSRKK"
     gene            3268..>3661
                     /locus_tag="VGT44_11085"
     CDS             3268..>3661
                     /locus_tag="VGT44_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581384.1"
                     /translation="MSNITSTSHQPRVLFMGMSGNFSHPSLLALLESGIDVCAVVLPA
                     RVNDTSQPAIRRLEPPAKPRAMLPVLSSSLHIGIARIAWERAVPLWEAQRMSDPETSA
                     VYASYAPDAFCVACFSLYIPRSILAIPPL"
BASE COUNT          746 a         1065 c         1146 g          704 t
ORIGIN      
        1 cagtccagtg tgagaactcc ttcatctgct gatgcgcttg attgtgagag taactctacc
       61 agagcttcag gggaaatagg atagcatcct ggtatcattc aggtagcaac attggagagg
      121 taggaattac aacaggctca atcccgctcc gaccagggca gctaatagca cgaccagcca
      181 gggtgggcat ttccagacaa cgagcaggcc gaaggcggcc agggcgaaca ggaagtcgat
      241 gggcgctttg atcgtgctgg tccagacggg ggtgtagagc gccgccagca gcagcccgac
      301 gacggcggcg ttgatgccgc gcagggctgc ctggaacggg ggctgtttgc gtagcgcgtt
      361 ccagaatggc agcgtgccaa tcaacagcaa gaaggatggt aggtagatgc ccagcaaggc
      421 gattatagcg cccaaccaac cgttgggggt aggattctca acggcgccca ggtaggctga
      481 gaaactgaaa agcggtcctg gaacggcctg ggccgcgcca tagccggaga ggaactggcc
      541 gttggtgacc cagctgggcg caaccaccgc cgattgtagg agaggcagca cgacatggcc
      601 gccgccaaag acgagcgcgc cgctgcggta gaagctggca aatagcgcga gggtatgtcc
      661 cggcactacc aggctaagca ggggcagcgc gagcagcagc acgaagaaga gcgccaggct
      721 gattattgcc agcgcgcggg ggattggtat atgctgtgca ggcgttgcca gggatgaaga
      781 cgaagttatg gtggtttcac cggtcttgcg caagaagagc cagccaatca aacctgcgac
      841 gacgatgatg actacctggc caatacccga tgcccagagc agcgtggcga tggtggccag
      901 gatggccata gtggcgcgtg ggcgatccgg cgcgaggttg cgcgccatgc cccagaccgc
      961 ctgggcgacg actgcgacag ccaccaccat caatccgtgc agccaaccgg tcgaggtcga
     1021 gacatgcagg acgctggtgg tataggcaaa gatggtcatg aggatggcgg acggcagcgt
     1081 aaagccgatc cacgcggcca gcccgccagg cagaccggcg cgcataatgc ccagcgagat
     1141 gccggtctgg ctgctggcag gacctggcag aaactggcac agacccacca ggtcgccata
     1201 ggcgcgctca tccacccact tacggcgcat gacgatctcc tcgcgaaaat agcccaggtg
     1261 cgcgaccggc ccgccaaaag atgtcagtcc gaggcgcgtg aagacgccga ggacttcgag
     1321 gacgctgccg cgctgcgtag cctggacgct atccagcgta ttttcctgag tttgcattcc
     1381 cgctcctcat catcgaaccg ggtcaaactg acccgcgacc ggctctgctc agcagaacca
     1441 cgtttcggtc aggtggggaa tgacttttcg cacaccgtag ccgccgatgc cgcaggaaac
     1501 gcacatgtcc tgtccctgca tcgcaagcgc cagggctttg atgtggaaga acgtttcgat
     1561 ggggccggct cccttgcgct gcgcctcttc gaccagcgcg cagatctggc tatgcagcat
     1621 gtccatgtgt ggatcgggat gttgccagcg atacgtatag gcggcggcgg ccaattctcg
     1681 cagccactcg cgcctgtcgg gtgcattgag cagcgcggaa ccgggcggga tcagcaagcg
     1741 aatggaaaga tgcaccgggt cgatgtgttc gatgaggtgg cgctcttcga tgaaattgag
     1801 catgcgtatg tagctcgcca gcgtttccca cggcgagaat ggcatcagcg aagggcgtag
     1861 agcgatgccg acctgctcca tcaactcgaa tgcatcggcg acatcggcgc tggtatgtcc
     1921 cttttccagg cgcgccagca cctcgtcgtt caatgattcg acggcggata cgacgaagat
     1981 gcagccgttt tgcttgaatt cgggcaacag gtgacggtgt tgcagcaggt gttcgatctt
     2041 aatggtcgcg tcgaacgtta tctctggaaa ttcctggtgc agcgcgtgcg tggtgcgcag
     2101 cgcgtgggtt ggtccgttca ggaaatcggg gtcgccaaag gtgatgtgct gcgcgccctg
     2161 ctgtatttgc gcgcgaatgt cggccagcac acgatcggca ggcgtggcga agaaacgccc
     2221 ctggtagacc ggtgtgatcg ggcaatgctg gcaggtatgc ttgcagccgc gcgtggtctc
     2281 cgcgtatccg acaatgcgcg tttcgccgtt ccactctagc cgcgcgtagc ggtcgagtgg
     2341 cggcaagcct gcgcgctccg gcatcaccag tggcgcgcgg acaatgcggg gattgctcaa
     2401 ctgctggcgt gtactgactc ctgggatagc cgaggttgtg ccgtcagggc tattttctag
     2461 cgcggcgacc agttctagca aaggctgctc gtattcgccg ccgatggccg agtcgatcaa
     2521 ctcgcgcagc aggtaatctt cgttcaacgt ggcatacagg ccatagaagc agatatgcgc
     2581 cgaaggattg agcagacgaa tacgccgcgc gagtagctct cccaggcgca gggccgtatg
     2641 catcggcacg gctatgccga cgaagcgtgc ctggatgatg gtatcgtccg ccaatggctc
     2701 aacggcggtg tcgacgacgg taggggcgta cccggcgcgc cgcaggagag cggtaagcgt
     2761 ggcgaggtgc agcggctgat gtcccaattc ataacaggaa agcagcagga gagcgccggg
     2821 tgatctcatt tcttgcgcga cggcgcgcct ccaccttttg tgctgggacc cacacccaca
     2881 ccttcatctt cctctggtgt ccagccttca ggcatcttgg gctgctcgcc aaagaaaaac
     2941 tcttccattt gctcgcgcag gattttacgc gcctccgggt cggccatatt caggccatag
     3001 tggttgataa tcagggtact ctgcgcgggc cacatatccc acgcctgttt cgagacgttc
     3061 tcataaatgc gctgccccaa ctcgccagga aacggcggct tatccagccc cggtagcgag
     3121 cgcccaagtt tgacacactg aacgatacgt gccatgataa aaactcctga tgctttctct
     3181 atggttgcgt cctcgcggat atcgattaac gcacaggaca gaatactact tcttcctaca
     3241 gccaaatctt atatgattga gtgacgtatg tcaaatataa catcgacatc ccatcagccg
     3301 cgcgtcctct tcatgggcat gtccggcaac ttctcgcatc cttctctgct tgctttgctg
     3361 gaaagtggga tagatgtgtg tgccgttgtg ctgcctgcgc gcgtcaatga tacctcgcag
     3421 cccgctatac gccgcctgga accgcccgcg aagccgcgcg ccatgctgcc agtgctgtcg
     3481 tcctcgctgc atatcgggat cgcgcgcatt gcctgggaac gcgcggtccc gctctgggag
     3541 gcgcagcgta tgtccgaccc cgaaaccagc gcggtctatg cctcctatgc gccggatgcc
     3601 ttctgcgtgg cctgcttctc gctctatatc ccgcgtagca ttcttgctat cccgcccctg
     3661 g
//
LOCUS       DASZAK010000632         1956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_265538, whole genome shotgun sequence.
ACCESSION   DASZAK010000632 DASZAK010000000
VERSION     DASZAK010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265538"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VGT44_11090"
     CDS             complement(<1..455)
                     /locus_tag="VGT44_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV2581385.1"
                     /translation="MSKTIWVLVELDNDRPARSSLEVLGKAASLGRAEAIVLGSQAAA
                     VAPTLGEYGAEKVYVHAEAAYDTYLTLPAVDTLSDLLKQRQPDLLMLATTYDLRDIAA
                     RLNVRNNMGLITDATDLQFEGDTLNVTVPWGGENIVTATHPQQGPGIVLT"
     gene            complement(568..1353)
                     /locus_tag="VGT44_11095"
     CDS             complement(568..1353)
                     /locus_tag="VGT44_11095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEV2581386.1"
                     /translation="MKMYVCIKQIPDPNTVVSKLDPNTKRLVRSGVSMVLDPGDESTI
                     SAAIKLRDTIGNSELVAVSMGPSSAQEAMRRALAMGADSAILVTDPSLAGSDALSTAR
                     VLAAVLKREHADLIFCSTESTDGYTGMVPGGIAEFLGIPQLTFAREIQIEGQKAIIKR
                     VTQTGYQTVECALPAVVTIASGSYEAIYPTMKGIMGAKKKPFVQLTLSDLGIDAAQVG
                     EAGARERVLAVGKAEARAAGQVLKDDGNAAQTIADFLQRNQLL"
BASE COUNT          370 a          544 c          602 g          440 t
ORIGIN      
        1 gtcaggacga taccgggtcc ttgctgcggg tgggtcgcgg tcacgatgtt ttcgccgccc
       61 caggggacgg tcacgttgag cgtgtcgccc tcgaattgca ggtcggtggc gtccgtaatc
      121 aggcccatat tgttgcgcac gttcaaccgc gcggcgatgt cgcgcaggtc gtaggtcgtg
      181 gccagcatca gcaggtcagg ctggcgctgc ttgagcaggt cgctgagcgt atcgacggct
      241 ggcagggtca ggtaggtatc ataggcggct tcggcgtgga cgtagacctt ttccgcgcca
      301 tactcgccca gcgtcggggc aacggcggcg gcctgcgagc caagcacgat ggcctcggcg
      361 cggccaagcg aggcagcttt gcccagcacc tcaagtgaag agcgcgcggg gcggtcattg
      421 tctagttcga ccagcaccca gatggttttc gacatatttc tattctcctc tatcgaacga
      481 tgtaggggcg caattcattg cgcccaagcc gattcgcgtg aagatgggcg caatgtattg
      541 cgcccctaca tcgcgtttgt agattggcta gagcaactgg ttgcgctgta aaaagtcggc
      601 gatggtctgg gcggcgttgc cgtcgtcctt caagacctgg cctgccgcgc gcgcctccgc
      661 tttgccgacg gccagcacac gctcgcgcgc gcccgcttcg ccgacctgcg cggcgtcgat
      721 tcccaggtcg ctcagggtaa gttgcacgaa gggctttttc ttcgcgccca tgatgccttt
      781 catcgtcggg tagatggctt cgtaggagcc gctggcgatc gtcacaacgg cgggcagcgc
      841 gcattcgacg gtctgatagc cggtctgtgt gacgcgcttg atgatcgctt tctggccctc
      901 gatctgaatt tctcgggcga aggtcaattg agggatgccc aggaattccg cgatgccgcc
      961 gggtaccatg ccggtatagc cgtccgtgct ttcggtggag cagaagatca ggtcggcatg
     1021 ttctctcttc agcactgccg cgaggacgcg cgctgtgctg agcgcgtcgg agcctgccag
     1081 cgaggggtct gtcaccagga tggcgctgtc cgcgcccatc gccagggcgc ggcgcatggc
     1141 ttcctgtgcg gaagaaggcc ccatactgac ggctaccagt tcgctattgc cgatggtgtc
     1201 gcgcagcttg atcgcggctg aaatggtgct ttcgtcaccc ggatcgagaa ccatcgatac
     1261 cccactgcgc acaagtcgtt tcgtattcgg gtcgagcttg ctgacaaccg tgttggggtc
     1321 cgggatttgc ttgatacaaa catacatctt catatgctag tgcctcaacc ttctactttt
     1381 ccgactggct gggcggagcc aggacgcagt ccgttgccag tcggaataag agaaccctat
     1441 ttgaacaaat atggaagcgt agccagggtg cgcccctgcg attgtgcggt cgcccaggtt
     1501 ttgatcggta gtttcctcgg ctatcttatt gtacctgtcg agaaaagagc atgtcaacgt
     1561 tgctggctaa cgagaacgcg ataatgaacc ttcccgacca ccatcgggaa tgtcatcctg
     1621 agcgcagcga aggatctcgt ccctggctag tgagatcctt cgctgcgctc aggatgacaa
     1681 gcctggtcgt cgatcttgtt tgtaaaagtt cattatcgcg ttcctacgac gaggcgttcc
     1741 tgtattcaga ttccattcag caaacgaggt ataatatgca ttgtatggtc tgattcgtga
     1801 gtgtgagcag ccagccgaac gaagatgggt atgaaagaca gcgaacctcc agacggcaac
     1861 caggatatgg acgaaagaca agacgatcag gaagtcgaga ttacgaatct tgatgatcca
     1921 ggggcgattt cgcgctcatc gtggctcgcc tcaatt
//
LOCUS       DASZAK010000633         1503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_584872, whole genome shotgun sequence.
ACCESSION   DASZAK010000633 DASZAK010000000
VERSION     DASZAK010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584872"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1180)
                     /locus_tag="VGT44_11100"
     CDS             complement(68..1180)
                     /locus_tag="VGT44_11100"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2581387.1"
                     /translation="MRRASGYTRLSVFLIMISMLFAACGTTTTGGTTPTTTAAAPITV
                     GLVTDIGGLNDGGFNQFSHQGYETARTKYNFPEVVIQSTVVSDAEYKKNLTAAAGQAD
                     LVIGIGFNMETAITAVAKQFPAKEFAIVDGCAIDANFNCINLPNVAPLFFKEQQAGCL
                     VGAIAGQMEVDGKSAAPKLLGKNTISAVGGQAIPPVNRYIAGYKYCAKLVDPTVNVVI
                     GYSNNFADPTPCAAIANKQISINQADILFQVAGGCGNGVLDAADQKGVYSIGVDADQS
                     KTSTGTVRPSVITSAIKRVDTAVDTIINLAEQQGKASSGAYSSFVTNPYKFDLTNSGV
                     GFATPSSQVPADAVAKANAFEAEIKAGTLTPPENIP"
BASE COUNT          300 a          417 c          415 g          371 t
ORIGIN      
        1 gtgcgaaaag ggcgtaccct tgcggtcgcc ctctggtact cctaccatcc gcccacctcg
       61 ggtttgctta cgggatgttc tccggtgggg tgagcgtccc ggccttgatc tccgcttcga
      121 atgcgttcgc tttcgctacc gcgtccgcgg gaacctggct gctcggtgtg gcgaaaccga
      181 cgccgctatt ggtcagatca aacttgtacg gattagtgac gaagctgctg taggcaccac
      241 tcgaggcttt gccctgttgc tccgccaggt tgatgatcgt gtcgacagcc gtgtcaactc
      301 ttttgatggc gctggtaatc acacttggcc tgacggtgcc ggttgaggtt ttgctctgat
      361 cggcatcaac gccgatgctg tagacaccct tctggtcagc cgcatccaga acgccattgc
      421 cgcagccacc agcaacctgg aagaggatgt cggcctggtt gatactgatc tgcttattcg
      481 caatagcagc gcacggagtg ggatcggcga agttgtttga gtaaccgatc accacgttta
      541 ccgtcggatc aacaagcttc gcgcagtatt tgtacccggc gatgtagcgg ttcaccggcg
      601 ggatggcctg gccacccacc gcgctgatgg tatttttgcc gagcagcttg ggcgcggcgg
      661 acttcccatc tacttccatc tgaccggcaa tcgcgcccac caggcagccc gcctgttgtt
      721 ctttaaagaa gagtggcgcg acattgggaa gattgatgca gttgaagttg gcatcaatgg
      781 cgcatccatc gacgatggca aattccttgg ccgggaactg cttggcaacg gcggtgatag
      841 ctgtctccat attgaagcct ataccgatca cgaggtcggc ctggcctgct gccgccgtca
      901 ggttcttctt gtattcggcg tcacttacga cggtcgattg aatgacgact tctgggaagt
      961 tgtacttcgt cctggcagtc tcgtacccct ggtgagaaaa ctggttgaag ccaccgtcgt
     1021 tcagtccacc aatgtcagtg accagtccca cggtgatagg cgcagcagcc gtcgttgtgg
     1081 gcgtcgttcc tcctgtggtg gtagtcccgc atgccgcaaa taacatggag atcatgatga
     1141 ggaataccga gaggcgcgtg tagccgcttg ctcttcgcat gtccgcttcc tttcttctct
     1201 tgtaaaaaat acaggtgtcg agttgttctc cataaatgaa caaggagtgt ctgataatcg
     1261 cagcccggtc gtaacggctc attgggcgat catctccact ccatctctcg taagaacaac
     1321 atgtcggaaa atgccctttc gcgtggctga gcctcacgcg gaaaaactct tcctgatatg
     1381 cttactacgt ctcaaaccgg cggattctca cagaagctga gaatgttcaa ccacataaaa
     1441 cggcgtatga tggcaacccg cgggttgcca tcatacgccg ttttatgtgg ttgaacattg
     1501 atc
//
LOCUS       DASZAK010000634         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_691452, whole genome shotgun sequence.
ACCESSION   DASZAK010000634 DASZAK010000000
VERSION     DASZAK010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691452"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VGT44_11105"
     CDS             <1..306
                     /locus_tag="VGT44_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581388.1"
                     /translation="RRIMELFEVCQEQGLTTRSSSSHTVSSRRVPFQKRAFSLGDVIG
                     VNAYRVNRDAPKQRLAEGGYQVHTTPHFLVCQQPGAPTIVVHWFPPAAIDGDLQRSA"
     gene            353..1642
                     /locus_tag="VGT44_11110"
     CDS             353..1642
                     /locus_tag="VGT44_11110"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV2581389.1"
                     /translation="MLTIDIKTARRFILGKQGLWPGRRWRGSEGTEAAIRAMEYLQLD
                     PLQILARSQDITLHSRVLDYTPGLWEELAYQQRKFFDWGGWLAVRPMEELPHWRVVMR
                     RERDGDYGDSRLRRMGREHVEAIEEMRAILRERGTVSNRDFAMASRKRTQSYRGRKDS
                     ALALYYLWCTGEVMTHHRENFERVYALTETVAPASLIRESDEAEADRFLIKKDVSFSG
                     LSLPSRVAESFRGYGESGHATKQLLGAMGADGDLIEVQVEGWKQVHYALGSDAAVLCE
                     LSAGRVPATWTPLETTTTQEVVFLAPLDHVSARGRAKVLFGFDYVWEVYKPEHRRKFG
                     YYTLPVLWGDRLVARFDSKLDRTTGTFVVLGLWLEDEAHGLDEAFAAALARGLARFMR
                     FLGASKLDATAMREPLLRQRACASEQSRSIPKQSDDV"
BASE COUNT          365 a          557 c          620 g          390 t
ORIGIN      
        1 agaaggatca tggaattgtt cgaagtgtgt caggagcagg gattgactac tcgttcgtct
       61 tcctctcata cggtttcttc caggcgggta ccttttcaga aacgtgcctt ttctctgggc
      121 gacgtgattg gggtcaatgc ctatcgcgtc aatcgtgacg cacccaagca acgcttagct
      181 gaaggtgggt atcaggttca tacaacgcct cattttctgg tgtgtcagca gccaggcgct
      241 ccaaccatcg tcgtacactg gtttcccccg gcagcaatcg atggcgattt acagcggagt
      301 gcttaaagat tgagctctct ttaactctcc cttttttgac caggaggtcc tcatgctcac
      361 catcgatatc aagaccgccc gtcgcttcat ccttggcaag cagggcttgt ggcccgggcg
      421 gcgctggcga ggcagcgagg gcaccgaggc tgctatacgc gccatggagt acctgcaact
      481 cgacccgttg cagatcctcg cgcgcagcca ggacattacg ctgcacagcc gcgtcctcga
      541 ttatacacct ggcctgtggg aagagctggc ctatcagcag cgcaagttct tcgactgggg
      601 tggctggctg gccgtccgtc caatggagga actgccgcac tggcgcgtgg tcatgcgccg
      661 cgagcgcgat ggcgactatg gcgactcgcg ccttcgccgc atggggcgcg aacacgtcga
      721 ggcgatcgag gaaatgcggg ccatcctgcg cgagcgcggc accgtgagca accgcgactt
      781 cgcgatggcg agccggaagc gcacccagag ttatcgcggt cgcaaggaca gcgcgctggc
      841 cctgtattat ttatggtgca ctggcgaagt gatgacgcac caccgcgaga acttcgagcg
      901 cgtgtacgcc ctcaccgaaa cggtcgctcc cgcatcactc attcgtgaaa gcgacgaggc
      961 cgaggccgac cgcttcttga tcaagaagga tgtcagcttc tccggcctgt cgctgccgag
     1021 ccgtgtggcc gagtcgtttc gtgggtacgg cgagtcgggt catgcaacta agcagttgct
     1081 cggggcgatg ggggccgacg gcgacctcat cgaggtgcag gtggagggct ggaaacaggt
     1141 gcattacgcg ctcggcagcg acgccgcggt gctgtgtgag ttgagcgcgg gacgcgtgcc
     1201 ggctacttgg acgccgttgg agacgaccac gacccaagag gtcgtcttcc tcgccccgct
     1261 cgatcatgtc agcgcacgcg ggcgagccaa agtgttgttc ggcttcgact acgtgtggga
     1321 ggtctacaag ccggagcacc ggcgcaagtt cggctactac acgctgccag tattgtgggg
     1381 cgaccggctc gtcgcgcggt tcgacagcaa actcgaccgg acgaccggca ccttcgttgt
     1441 gctgggcctg tggctggagg atgaggctca cggccttgat gaggcctttg cggcggcatt
     1501 ggctcgcggg ttggcacgct tcatgaggtt tctcggcgcg agcaagctgg atgccactgc
     1561 gatgcgcgag ccgctgctgc gccaacgcgc ctgcgcatct gaacagtcac ggtctatccc
     1621 caagcagtct gatgatgtat gaccacgcct aagctacatg aaagctcgac cctttggcac
     1681 tccctgtagg aagagccgca tttccaccgg ccttgacagc tccttgcctc tgaaggataa
     1741 ggagagtcga gagtggtgtc aaacgaggca gatcagatgc ctaggtctgg tgcggacacc
     1801 tggactgcca ggatgaacgt gcaacgagca tgcgccagag gaaggcttct tgcggctgct
     1861 acggtctggg ttgactggga aagagggcgg gcagatggcg ttctcgatgc agcaattgcc
     1921 gccattgata gc
//
LOCUS       DASZAK010000635         5260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_691467, whole genome shotgun sequence.
ACCESSION   DASZAK010000635 DASZAK010000000
VERSION     DASZAK010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691467"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..157)
                     /locus_tag="VGT44_11115"
     tRNA            complement(87..157)
                     /locus_tag="VGT44_11115"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Cys,seq:gca)
     gene            complement(171..242)
                     /locus_tag="VGT44_11120"
     tRNA            complement(171..242)
                     /locus_tag="VGT44_11120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(208..210),aa:Gly,seq:gcc)
     gene            complement(317..2728)
                     /locus_tag="VGT44_11125"
     CDS             complement(317..2728)
                     /locus_tag="VGT44_11125"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEV2581390.1"
                     /translation="MNSNAGARLRYCMGTNSMEQSDLYLLQHVPSYHLQAVSKVRQLP
                     LAVSAQSAKSTGDSVQIESDKLRELGEQLFDPTACREVIHSLEASDLLILQELTSCGG
                     RANSRDLALYMSSASPASPIAHSSSTPHAPQYPTPHPHGAFEQAVHRLLTLGLLFWGK
                     QTSFSGRDYANGVYDGILIVPQAVMDAVQSIESEQSARVEQESLEMLLSDELHELQRS
                     LYLYWSLAAAQRDGLPLVSSKLLSRPALRQVVEQLTTLNVEQIRIEADVPRLLFIRLL
                     MTRLGLLYERNSSIYPAPVETVEGYFALTLAERARRCYRLWLDSPFWNELNYLPGVLL
                     RPTPGPTDPTHAEVARARAKVMEQVLQAKAGSPQALVTFITRAKLHMPYLLFPRDYGA
                     RTERYSIGSNPYGLDFRLKHGWLTPREGWHLVEGGFIRAVLSGPLHWLGLVDVDEDTD
                     TFTPLPALAPIAGDSDEQARDATTEVWGRLVVQPNFEMVALAPVSEALLLRLDRFAER
                     IRLEQIAQYRLTRTSVTRAVQTGVSAEEILRLLEQAAGGAIPQNVYYSLKEWERYARR
                     VELWTHMTLIEVESEATLDAFYEDDALRPLFGRRLSPTLAQVAADQLQTLQERLWERD
                     LLPALAAAPTYTNVLESGGRMPPHEAQWRLLEEGLMQPCYAVLNLYLAAELDRFTDLD
                     ETTDWRRITPASIQQARDNGLSLDAIARFLQRYCEGGLPASFLIRLKLWGGGYQQQEI
                     IQVETTPMLRLSASALRDIQADEELGVLLEAEVPSESRLVRVSPDSLARVLALLRERG
                     FTVDD"
     gene            complement(2679..5237)
                     /locus_tag="VGT44_11130"
     CDS             complement(2679..5237)
                     /locus_tag="VGT44_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HEV2581391.1"
                     /translation="MYDIVPGALQHLREADIISMAGLAVAAQGQEYSRTSAVHSTHRQ
                     GSRLSGIVDVGGLPSKADAIGTNAAKAVEFPGMTLQSFPVTVEINDANTWHFTCACTP
                     VNSSSLRLCVHAAALLYYWLARPFAFAASPTAATPSIPSAPSSSLMQPMLPTKPALST
                     PTVAPVQPTKTADSLALLAPPDLRTIAKEYELASNGLNKQEMIEAILGVFRQPEVVRR
                     MVGTLEKMQRQFLATFTLAGGYMSDQELRGLFERFALGKQEQYQQAMAKLQSKGLILR
                     ASFNSTQQLRAGLGGSSNLSNLNPLEVTWHVPPEVCSALTITLPVAPFRAEEQSAPPV
                     VYHAEPYRLLADLLLVARVLEGQLTPREEKSAGRRGITGSLMRAVQASPSPSSASPSP
                     EGSVAIPPPAGLLPSALLETLQGAVTLSPAFLRFAYRILRLGEILYTETDAPQAASTT
                     NGASVTLRMLPNAAELLLGPTRSDVTLELFSHWLRQATYIELFELSELGLRLRCRTIA
                     PNQPALRQGELEAENREARQWLISLLAQAPLRQWISFPAFARFVFRLYPTFLQRRQHL
                     FPTPHWWIEQDEGRPLHPEQWNDWQRADARYLAQLLQGPLYWWGTCDIALTPGGQLQA
                     FRLTPLAEHFLHDPGSAHLFLGFSADQRSDASTDPVALDSLGAGPEEQEAHPTSHTEP
                     VEPAMYDMSGIELSEQGDVLVISSAGNWPLIELVERFTRVTGVQAGRLRYQITPAALS
                     EALRRGEDLAPFVELLRCARDAGQGNGRVEAMLSRLEQRLASYGRIRLYTGATLLAAA
                     DTLTLRELAATTSIEQQTIQAVHPTLLVLKKQGSEQLIDELKRRGQAPLLHGDE"
BASE COUNT         1043 a         1496 c         1722 g          999 t
ORIGIN      
        1 cgtaactatg ttggtgtctc attagtgcct catgggcgtg catctcaaaa agggggacat
       61 gaactacttg atcacgtagt cttgggaggc gacgagcgga ttcgaaccgc tggtgcagct
      121 tttgcagagc cgtgccttac cacttggcga cgtcgcccag aacaaaaggg agcgggagac
      181 ggggatcgaa cccgcgactt tctccttggc aaggagatgc tctaccactg agccactccc
      241 gcatataact tacgtttgca caagtaagat atcatacccg gtgggttttg tcaactggtt
      301 tttcgactcg acttgttcag tcatctacgg tgaagccgcg ctcgcgcagc aaggccagga
      361 cgcgcgcaag ggagtccggg gagacgcgca ccaggcggct ttcggatggg acttccgctt
      421 cgagcagtac gcctagctct tcgtcggcct ggatgtcgcg caaggcgctg gccgagaggc
      481 gcagcatggg ggtggtttca acctggatga tttcttgctg ctgatagccg ccgccccaca
      541 gtttcaggcg aatgaggaac gaggcaggca gaccgccctc gcagtagcgc tggaggaagc
      601 gcgctatggc gtcgagcgag aggccgttgt cgcgcgcctg ctggatggag gcgggcgtta
      661 tgcgccgcca gtcggtcgtt tcgtcgaggt cggtaaagcg gtcgagttcg gcggccaggt
      721 agaggttgag gacggcgtaa cagggctgca tcaacccctc ttccagcaag cgccactgcg
      781 cctcgtgggg tggcatgcgc ccgccgcttt ccagcacatt ggtgtaggta ggagcagcgg
      841 cgagcgcggg cagcaggtcg cgctcccaga gtcgctcctg tagcgtttgc agttgatcag
      901 ctgccacctg ggccagcgtg ggggagaggc gtcgcccgaa gagaggacgc agcgcgtcgt
      961 cttcgtagaa tgcatcaagc gtcgcctcgc tttcgacttc aatgagcgtc atatgtgtcc
     1021 aaagctcgac gcggcgggcg tagcgctccc actctttgag cgaataatac acgttctgcg
     1081 ggatcgcacc gccggcggcc tgttccagca ggcgcagtat ctcttccgct gagacgcctg
     1141 tctgcaccgc gcgtgtcacg ctggtgcgcg tcaggcgata ttgcgcgatc tgctcgagtc
     1201 gtatgcgttc ggcaaagcga tccaggcgta agagcaaggc ttcggagacg ggcgctaacg
     1261 cgaccatctc gaaatttggc tgcacgacca gccgacccca gacttcagtt gtggcgtcgc
     1321 gcgcctgctc gtcgctatcc cctgcgatgg gtgcgagcgc gggcagcggc gtgaaggtgt
     1381 ccgtgtcttc atccacgtcc accagcccca accagtgcag ggggccggag aggacagcgc
     1441 ggatgaagcc gccctcaaca agatgccatc cctcgcgtgg cgtcaaccag ccgtgtttga
     1501 ggcggaaatc caggccgtag ggattgctgc cgatcgaata gcgttcggtg cgcgcgccat
     1561 agtcgcgggg gaacaggaga tacggcatat gcagcttggc gcgcgtaata aacgtgacaa
     1621 gcgcctgtgg agagccagct ttcgcctgca agacttgctc catcaccttc gcgcgcgcac
     1681 gcgccacctc agcgtgggta ggatcggtcg ggccaggcgt gggacgcagg agaacgccgg
     1741 gcaggtaatt cagttcgttc cagaaggggg aatccagcca gaggcggtag caacggcgcg
     1801 cgcgctccgc cagggtcagg gcaaaatagc cctcgaccgt ctcgactggc gcgggataga
     1861 tggagctatt acgttcgtag agcaatccga ggcgcgtcat aagcaggcga atgaagagca
     1921 ggcgcggcac atcggcctcg atgcgaatct gctccacatt cagcgtcgtc aactgctcga
     1981 caacctggcg cagggcgggc cgcgagagca gtttgctgct caccaggggc agaccatcgc
     2041 gctgcgcggc ggccagcgac cagtagagat agagcgagcg ctgcaactcg tgcagttcgt
     2101 cactcagcag catctccagg ctttcctgtt ccacgcgcgc actttgctcc gactcgatgg
     2161 attgcacggc atccataact gcctgcggga cgatgagtat gccgtcgtag acaccgttcg
     2221 cgtagtcgcg ccctgagaaa ctggtttgtt tgccccagaa gagcaagccc agagtcagca
     2281 ggcgatgcac agcctgctca aatgcgccgt ggggatgtgg tgtgggatac tgcggcgcat
     2341 gaggagtaga ggacgagtga gcaataggag atgcgggtga agcgctgctc atatacaggg
     2401 ccagatcgcg gctattggcg cgcccaccgc aggaagtcaa ttcctggaga atcagcaggt
     2461 cggacgcttc caggctgtgt atgacctcgc gacaggcggt gggatcaaat aattgctcgc
     2521 ccaactcgcg cagtttgtcc gactcgatct gcacagagtc gccggtcgat ttagcactct
     2581 gcgcggagac ggctagaggg agttggcgta ccttgctcac cgcctgtaag tgataagatg
     2641 gcacatgctg caacagatac aggtcgcttt gctccatgct attcgtcccc atgcaatagc
     2701 ggagcctggc cccggcgttt gagttcatcg atcaactgtt cgctgccctg tttttttagc
     2761 accagcagcg tgggatgcac ggcctggatg gtctgttgtt cgatgctggt ggtggcggcc
     2821 agttcgcgta gggtgagggt atcggcagcc gcgagcagcg tcgcgcctgt gtagagccgg
     2881 atgcgcccat agctggcgag gcgctgctcc aggcggctca gcattgcctc aacgcggccg
     2941 ttgccctgac cggcgtcgcg cgcgcagcgc agtaattcca cgaaaggcgc cagatcttcg
     3001 ccgcgccgca gcgcctcgct caaggcggcg ggcgtgatct ggtagcgcag ccgaccggcc
     3061 tgaacgccgg tcacgcgcgt aaagcgctcg accagttcga tgagtggcca gttcccggct
     3121 gaggatatga ccagtacgtc gccctgctcg gatagttcga tgccgctcat gtcgtacatg
     3181 gccggttcga cgggttcggt atgactagtc ggatgtgcct cctgttcttc cggcccggct
     3241 ccgagggagt cgagggcgac cggatcggtg gatgcatcgc tgcgttgatc cgcggagaag
     3301 cccaggaaga gatgggcgct gccggggtca tgtaagaaat gttcggccaa cggtgtcagg
     3361 cgaaacgcct gcaactggcc gcccggcgta agcgcgatgt cgcatgtacc ccaccagtag
     3421 agcgggcctt gcagcaattg cgcgagataa cgcgcatcgg cgcgctgcca gtcgttccac
     3481 tgttcgggat gcaacggacg cccctcgtcc tgttcgatcc accagtgagg tgtgggaaag
     3541 aggtgctggc ggcgctgcaa aaacgtggga tagagacgaa aaacgaagcg ggcaaaggct
     3601 gggaagctga tccattggcg cagcggggcc tgggccagca gggagataag ccactgccgt
     3661 gcctctcgat tctcggcttc caattcaccc tggcgcaggg cgggctggtt gggtgcaatg
     3721 gtgcgacagc gcaggcgtaa gcccaactct gacagttcaa agagttcgat ataggttgcc
     3781 tggcgtagcc agtgcgaaaa aagctcaagt gtcacgtcgc tgcgcgtcgg gccaagcaac
     3841 aattcggcgg cattgggcaa catgcgcagc gtcacgcttg cgccattggt tgtgctggcg
     3901 gcctgtggcg catcagtctc ggtatagagg atttcaccca ggcgcaggat gcgataggcg
     3961 aagcgcagga aggcgggcga aagcgtcacg gcgccctgca acgtttccag cagagcggac
     4021 ggcagcagac ccgccggcgg cggaatagct acagaacctt ctggagatgg cgaggctgac
     4081 gaaggtgatg gagaagcctg tacggcgcgc atcaggctgc cggtgatgcc cctgcgcccg
     4141 gcgcttttct cctcacgcgg cgtcaactgg ccttccagga cgcgcgcaac gagcagcaga
     4201 tcggccagca agcgataggg ttcggcatga tacacaaccg gcggagcgga ttgttcctcg
     4261 gcgcgaaaag gcgcgaccgg cagggtgata gttagagcgg agcagacctc cggcggcacg
     4321 tgccaggtaa cttcgagcgg attcaagttg ctcagattgc ttgatccacc cagccccgcg
     4381 cgtaactgct gcgtgctgtt gaaactggcg cgcaatatca gtcccttgct ctggagcttt
     4441 gccatcgcct gctgatactg ctcctgtttg cctaatgcga aacgctcgaa caggccgcgc
     4501 agctcctggt cgctcatgta cccaccggcc agggtgaagg tcgcgagaaa ctggcgctgc
     4561 attttttcca gcgttcctac cattctgcgc acgacttccg gctgtctgaa cacgcctaga
     4621 atggcctcga tcatctcctg cttattcaac ccgttgctgg caagctcgta ctctttggcg
     4681 atggtgcgca aatcaggcgg agccaggagc gcaagcgaat cggcagtttt ggtaggctga
     4741 acgggggcga cagtaggagt tgacagagcc ggtttggtcg ggagcattgg ctgcattaaa
     4801 gaggaggatg gagcagaggg gatcgaagga gttgcagcgg tgggggacgc ggcaaacgcg
     4861 aaggggcgcg cgagccagta gtagagcagc gcggcggcat ggacacataa gcgaagcgaa
     4921 gaggagttga caggagtaca ggcgcaggtg aaatgccagg tattcgcatc gttgatttca
     4981 accgtcaccg ggaaactctg aagcgtcatt ccaggaaatt ctaccgcttt cgccgcattt
     5041 gtgcctatcg catctgcttt tgagggcaaa ccacccacat cgacgatgcc agaaagtcgc
     5101 gagccctgac gatgggtgga atgcacggcg ctcgtgcgac tatattcttg accctgcgcc
     5161 gctacagcca ggcccgccat actgatgata tcagcttctc gcaggtgttg taacgcgcca
     5221 ggcacgatat catacatgtt tcctgcctgc tacaatgctc
//
LOCUS       DASZAK010000636         1767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_122169, whole genome shotgun sequence.
ACCESSION   DASZAK010000636 DASZAK010000000
VERSION     DASZAK010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122169"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..>1767
                     /locus_tag="VGT44_11135"
     CDS             37..>1767
                     /locus_tag="VGT44_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV2581392.1"
                     /translation="MENESYEPGSGNIADAAHQQARQIARTRVVGQKERRVDARPLVT
                     GAPVYAAEFEQPNMLHARILHSPHAHATIRSIDKSKALALPGVYAVLTYEDLPRIAHS
                     TAGQPYPETSPYDAYLLDSRVRYVGDWVAFAAAETPAMAEEALGLIEVDYELLPAVFD
                     PLEAMREGAPQLHEPDATHPNAGKHKGDIYDAAHNIAAHEVIEHGDFEAALREADIIV
                     EGEYRVPFISHAVLEPHVCVAYLDGYERMIVVSSTQVPYHTRRQLAAVLQLPISRVRI
                     VKPRVGGGFGSKQEMLLEPVAAALALKTRLPVRIEYSRQEEFTAGRFRHPVIMRMRSG
                     VKRDGTLVAISMHSIGNAGAYGTHSFTVTRSTGHKTLCLYRAKAYRFRADAVYTNLPI
                     TGAMRGYGAPQGFFALESHIDEIAHRLNMDPLEFRRKNHVQKGDWDPIEGEEIDGVWH
                     SKRQFRSCGLPQCLEKGTAAIGWYEPFDRGDGKPIRRGRGMATAMQGSGVASFELGGA
                     SIKLNEDGSFNVMTGATDIGQGSDTILAQIAAEALGVGMDKIIMHSVDTDSSVFDYGS
                     YASSTTYISGG"
BASE COUNT          387 a          501 c          519 g          360 t
ORIGIN      
        1 cgatccagca gcctgacaag tttcagagaa tatttaatgg aaaacgaatc ttacgaacct
       61 ggctccggta acattgccga tgctgcacat caacaggcac gtcagatagc gcgtacgcgc
      121 gtggttggac agaaggaacg gcgcgtggat gcgcggccgc tggttacagg cgctcccgtc
      181 tatgcggctg aatttgagca gcccaatatg ctccatgctc gtatcctgca ctcgccgcat
      241 gcgcatgcaa ctattcgcag catagacaaa agcaaggccc tcgctctacc aggcgtatat
      301 gctgtgctca cctacgaaga tctgccccgg atagcgcaca gcaccgcggg ccaaccatat
      361 cccgaaacgt caccctatga tgcctatttg ctcgacagcc gcgtgcgtta cgtgggggac
      421 tgggtcgcct ttgctgccgc tgaaacgcca gctatggccg aagaagcact cggtctgatt
      481 gaggttgatt acgagctgtt accagcagta tttgatccgc tcgaagccat gcgcgaaggc
      541 gcgccccagt tgcatgagcc agatgcgaca catcccaatg cgggcaaaca caagggcgat
      601 atttacgatg cggcacataa catcgcagcg cacgaggtca tcgaacatgg cgattttgag
      661 gccgctctgc gcgaggccga tatcatcgtc gaaggagaat accgcgtccc tttcatctcg
      721 catgccgtgc tggaaccaca tgtctgtgtt gcctacctgg atggctacga gcggatgatt
      781 gtcgtcagca gcacgcaggt accctatcac acgcgccgtc aactggcagc tgtgctgcaa
      841 ctgcccatct cacgcgtgcg cattgtcaag ccgcgcgtgg gcggtggttt cggcagcaag
      901 caggagatgc tgctggaacc tgttgccgca gccctggcgc tcaagacccg gctgccggtg
      961 cgtattgagt acagccgcca ggaggagttt acggcagggc gtttcaggca tccggtgatt
     1021 atgcgtatgc gcagtggtgt caagcgcgat ggcacattag tggctatttc aatgcattcg
     1081 ataggcaatg ccggtgcgta tggtacacat agcttcacag ttacccgcag taccggccac
     1141 aaaacgttgt gcctgtaccg cgcaaaagcc tatcgcttcc gcgctgacgc tgtctacacc
     1201 aatctaccca tcaccggggc catgcgcggc tacggcgccc cacagggctt cttcgcgctg
     1261 gagtcgcata tcgatgagat tgcccaccgt cttaatatgg acccgctgga gttccggcgc
     1321 aagaatcatg tgcaaaaagg ggattgggat cctatcgaag gcgaggaaat tgatggagta
     1381 tggcactcga agcgccaatt ccgttcctgc ggcctgccgc agtgcctgga aaaggggacg
     1441 gcggcgattg gttggtatga accgtttgac cggggcgatg gcaaacccat ccggcgcggg
     1501 cggggcatgg caacagcaat gcaggggagc ggggtcgcca gctttgaact cggaggcgct
     1561 tcgatcaagc tcaacgaaga tggctcgttc aatgtaatga ccggcgcaac tgatatcggg
     1621 caggggagtg ataccatcct cgctcagatt gccgctgagg cgcttggcgt gggcatggac
     1681 aagatcatca tgcactcggt cgataccgac tccagcgtat ttgactacgg ttcgtatgcc
     1741 tccagcacta cctatatcag cggcggt
//
LOCUS       DASZAK010000637         4333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_514154, whole genome shotgun sequence.
ACCESSION   DASZAK010000637 DASZAK010000000
VERSION     DASZAK010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514154"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VGT44_11140"
     CDS             <1..467
                     /locus_tag="VGT44_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV2581393.1"
                     /translation="EIETLNVAIKSESQLIKIIERVEVESEGPSLEDASVIVSGGRGL
                     GKAENYHFVEELAAALGGAAGATRAIVDAGWLPYSYQIGQTGKTVKPTLYIACGISGA
                     IQHLAGMKGSKYIVAINKDEHAPIFSAADLGIIGDTLTILPQLTAEVKRRKQ"
     gene            489..2624
                     /locus_tag="VGT44_11145"
     CDS             489..2624
                     /locus_tag="VGT44_11145"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEV2581394.1"
                     /translation="MTPPAVSPTGGLIGTIIFVLVFLAALILFGVRAGRLIALLVKAR
                     RENRTDHIDDRIGEFFLIVLGQGGVLRDPIPGIAHFFTFWGFIIVQFGLLNLMLGAFN
                     ASLPLIGGNHGFAIALDAFVIFVLVALALFAIRRGIIRPRQLQSKLHGPWDGFIILGL
                     IFAVILTLALVESFEYAASNGAAWTPIGSWIGPAFTGFGVAANTAAFRIFWWAHILTV
                     LFFLVYLPRSKHLHLLATPFNVFFRNHGAKGALPLMENIEERDDYGVSKPEQFTWKQL
                     LDGYACTECGRCNTVCPASNTGKPLFPKEIILGVKEALLVQSDAILGEHSLWNRLGGK
                     SGEVGENISEKEASHLPMVGGIISTEALWSCTSCRACMEICPVSIEHVPTIVDMRRHL
                     VMEESEFPQEVTPVFNNIERNANPWEISNDKRAEWAAGLNVPLMSENPDADVLYWVGC
                     MGSFDERNKKVSTAVTKILQAAKVNFAILGPEESCTGDPARRIGNEYLWQMMAQQNIE
                     TLNGYGLNVNNQRDAMNGHAHGNGVAGAAPKHRTIITACPHCFNTIKNEYPQLGGNYD
                     VVHHTVFIDKLLSDEKITLPEGFDQRKLTYHDPCYIGRYNDIYDEPRRVLTVLNSNGV
                     TEMRRHRNKSFCCGGGGGRAWMEERIGKRVNQTRVNEALETGAEVLAAACPFCITMFE
                     DGVKGVEAEEKIKIEDISEIIARAIEFQQ"
     gene            complement(2753..3190)
                     /locus_tag="VGT44_11150"
     CDS             complement(2753..3190)
                     /locus_tag="VGT44_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581395.1"
                     /translation="MRLYTQLKVGFVGVFSCLALLLGMFSFTGIALAHSANDTQNPEI
                     SVTPKVVEPQQGCVEVTINGEDFRPSHKDDANHAQFFASDEDGRTFDVNPSSVRVNED
                     GQFSVDVNICEHLHKAEHHTVFVSAEDDATGRASNTVSFKLDF"
     gene            3500..>4333
                     /locus_tag="VGT44_11155"
     CDS             3500..>4333
                     /locus_tag="VGT44_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2581396.1"
                     /translation="MQSSVRDYWSVWKQFVLHGAMQGDVLPPAVLQSWRRCAAAGLDA
                     YSTRATYDAPELSLLSASSPSTLLALVRPAIEDLYQFVEGSECVVVFANTDARIIDLV
                     GDSTTQEHLGHLGVSVGATWHEERQGSNALALALRESFPIQLDGAMHYRAALHHIYTS
                     AAPVHDLMGQTVGVIGVIGPHEHCHPHTLGMITAAAQAISNQLQMQVWLSNANDLLSE
                     LRTILQSLPEGILLLRRDGMISQMNTPAGTMLGLMPAKVTGRRIHDILPLPTALAQAF
                     TT"
BASE COUNT          991 a         1251 c         1146 g          945 t
ORIGIN      
        1 cggaaatcga gacgctcaac gtcgccatca agtccgaatc acagctcatc aagattatcg
       61 aacgagttga ggtagaaagc gaaggaccat cgctcgaaga cgccagtgtg atcgtgagcg
      121 gcgggcgtgg cctgggtaaa gccgagaact atcacttcgt ggaggaactg gctgccgcgc
      181 tcggcggcgc ggcgggcgcg acccgcgcca tcgtcgatgc cggctggctg ccctacagct
      241 accagatcgg tcagaccggc aagaccgtca aaccgacgct ctacatcgcc tgcggcatca
      301 gcggcgccat ccagcacctg gcgggcatga aaggctcaaa gtacatcgtc gccatcaaca
      361 aagacgaaca cgcgcccatc ttctccgccg ccgaccttgg catcatcggc gacaccctga
      421 cgatcttgcc gcagctcacc gcggaagtga agaggcgaaa acagtaatca aaaacggagg
      481 ttcatcgtat gaccccccca gcagtctcac ccaccggcgg gttgatcgga accattattt
      541 tcgtgctggt cttcctggcc gcgctcatcc tcttcggagt gcgcgcgggc aggcttattg
      601 ccctgcttgt caaagccagg cgcgagaacc gcaccgacca catcgatgat cgtatcggcg
      661 aattcttcct gatcgtgctt ggtcagggcg gcgtactgcg cgaccctatt cctggaatcg
      721 cgcacttctt caccttctgg ggctttatca tcgtccagtt cggactgctc aatctgatgc
      781 tgggcgcgtt caacgcctcg cttcctttga tcggcggcaa tcatggcttc gccatcgcgc
      841 ttgacgcctt cgttattttt gtactggtcg ccctggcgct gttcgctata cggcgtggca
      901 tcattcgtcc caggcagttg caaagtaagc tgcatggccc ctgggacggc ttcatcatcc
      961 tgggactgat cttcgccgtc atcctcacgc tggcgctggt cgagagcttc gagtacgccg
     1021 ccagcaatgg agccgcctgg acgcccatcg gcagctggat aggcccggcc tttaccggct
     1081 tcggtgtcgc ggccaatacc gccgcgttcc gcatcttctg gtgggcgcat atcctgaccg
     1141 tcctcttctt cctggtctat ttgccgcgtt ccaagcacct gcacctgctg gcaacgccct
     1201 tcaacgtctt cttccgcaat cacggcgcaa agggcgcgct gcctttgatg gagaatatcg
     1261 aagagcgcga cgactacggc gtttccaaac ccgagcagtt cacctggaag cagttactcg
     1321 acggctatgc ctgtaccgaa tgcgggcgct gcaataccgt ctgcccggcc tccaataccg
     1381 gcaaaccgct cttcccgaaa gaaatcatcc tgggcgtcaa ggaagccttg ctggtgcaga
     1441 gtgacgccat cctcggcgaa cattcgttgt ggaaccggct gggcgggaag agtggagagg
     1501 tcggcgagaa catcagcgaa aaggaagcat ctcacctgcc aatggtgggc ggcatcatct
     1561 ccaccgaggc gctgtggtcc tgcacgagct gccgcgcttg catggaaatc tgcccggtct
     1621 ctatcgagca cgtgccgacg atcgtcgata tgcgccgcca cctcgtcatg gaagaaagcg
     1681 agttcccgca ggaagtcacg ccggtcttca acaatatcga acgcaatgcc aacccctggg
     1741 agatcagcaa tgacaaacgc gccgagtggg ccgccggcct gaacgtcccg ctcatgtctg
     1801 agaatcccga tgccgatgtc ctctactggg tcggctgtat ggggtccttc gacgagcgca
     1861 acaagaaggt ctcgaccgcc gttacgaaga ttctgcaagc ggcaaaggtc aacttcgcca
     1921 tcctcggccc ggaggagtcg tgtacgggcg acccggcgcg gcgcatcggc aacgaatacc
     1981 tgtggcagat gatggcgcag cagaatatcg agacgctcaa cggctacggc ttgaatgtca
     2041 acaaccagcg cgacgctatg aatggtcacg cgcatggcaa cggcgtcgcg ggtgccgcgc
     2101 cgaagcaccg caccatcatc accgcctgcc cgcactgctt caacaccatc aagaacgagt
     2161 acccacagct gggcggcaac tacgatgttg tccatcacac tgtcttcatc gacaaactgt
     2221 tgagcgatga gaaaatcacg ctacctgaag gcttcgacca gcgcaaactg acctatcacg
     2281 acccctgcta cattggccgc tacaacgaca tctacgacga gccgcgccgc gtgctgaccg
     2341 tcctcaatag caacggcgtg acggagatgc gccgccatcg caacaagagt ttctgctgcg
     2401 gcggcggcgg tggccgcgcc tggatggagg agcggatcgg caagcgcgtc aaccagaccc
     2461 gcgtcaacga ggcgctggag accggcgcgg aggtgctggc cgccgcctgc cccttctgca
     2521 tcaccatgtt cgaggatgga gtgaagggcg tagaggccga ggagaagatc aagatcgaag
     2581 atatctcaga gattattgct cgcgcaatag aattccaaca ataaagatgt agttcttttc
     2641 tcgaaaagaa tttgatgaaa agtcgcctac caaaaacatg ccagtgaagg agtatccttc
     2701 actggcaccg ctctttcact agacatcaaa agcagccgag gaagtcaggc tactagaagt
     2761 cgagtttgaa cgaaacagtg ttagaagcgc gtcctgtcgc atcatcttct gcactgacga
     2821 agaccgtgtg gtgctccgct ttgtgcaggt gttcacagat gttgacatcg acggaaaatt
     2881 gcccatcttc gttgacccga acactactgg gattgacatc gaaagtacga ccatcttcat
     2941 cagaggcgaa gaactgagcg tgattagcat cgtccttatg agaggggcga aaatcctctc
     3001 cgttgatggt gacttccaca catccctgtt gcggttcaac tactttgggc gtgacactga
     3061 tttctgggtt ttgagtatcg ttcgcactgt gcgcgagtgc gatgcccgta aaagagaaca
     3121 tacctaagag gagtgcgaga cacgagaaaa cgcctacaaa gcctacttta agttgcgtat
     3181 acagtcgcat gtgtccttct cactttctga tagaagagac aggggtattg aaccactatc
     3241 actccaaaag aataccctga tagctccaca gtgacgttct ataagaagac ttcctacatt
     3301 actctatgct ctagacagtt tctgccaaat ttagcacctt ttagtatacg actgatagct
     3361 atcaaggtta catatcggca tatattctct ggtgaggaaa ctccttctat tttgatgagc
     3421 atagcatgtg ctatactatg cacgagtttg ttagctcctt catctcgtca ttatagctct
     3481 tgcttgagga actgagaaca tgcaaagtag cgtgcgtgac tactggtcag tatggaaaca
     3541 attcgtccta catggtgcta tgcagggtga tgttttgccg ccagccgtat tgcaatcatg
     3601 gcgtcgttgt gcagccgctg gtctagacgc ctacagcacc agagcaacct atgacgcccc
     3661 tgaactatct ctcctttctg cctcttctcc gtctacgttg cttgctctgg tgcgacccgc
     3721 aatagaggac ctataccaat tcgttgaagg ctcggaatgt gtggtcgttt ttgccaacac
     3781 tgatgcgcgt atcattgatc tagtaggcga ctccacaact caggagcatc tcggacacct
     3841 cggcgtctct gtcggtgcta catggcatga agagcgccag ggatcaaatg ccttagcttt
     3901 ggctctacgc gaatcgtttc ccatacagct cgatggagcc atgcactatc gggcagcact
     3961 tcaccatatt tatacttctg ccgctcctgt gcatgatttg atggggcaaa cggtaggggt
     4021 cataggtgtg attgggccgc atgaacattg tcacccacac acactaggca tgattactgc
     4081 cgcggcacaa gctattagca atcagctaca aatgcaggtc tggctaagca atgccaatga
     4141 tcttctctca gagttacgca ctattttgca aagccttccg gagggtattt tgctgctccg
     4201 tagagatgga atgatttcac agatgaacac tcctgctggc accatgctag gactcatgcc
     4261 agccaaagta acaggcagac gcattcacga tatcctacca ttacctacag cgctagccca
     4321 ggcgttcaca aca
//
LOCUS       DASZAK010000638         8784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_265630, whole genome shotgun sequence.
ACCESSION   DASZAK010000638 DASZAK010000000
VERSION     DASZAK010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8784
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265630"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VGT44_11160"
     CDS             complement(<1..305)
                     /locus_tag="VGT44_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581397.1"
                     /translation="MGFTIQRALPTQGIFTQRLRRRWSRDVTIIIFLLPSMLLFFLFV
                     VFPVIQAAHYSLYRWSGLGDLTDYIGFKNYQALFEDPIFLQSLRNNVLIAVLSLVLQL
                     "
     gene            complement(536..1819)
                     /locus_tag="VGT44_11165"
     CDS             complement(536..1819)
                     /locus_tag="VGT44_11165"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2581398.1"
                     /translation="MKRNRVLLSLLAIMAILMVACGSGGNGSSGSGKVTINWWHINTA
                     DPLLSGWKNLAYEYMQSHPNVQINITVIGNDPFKTKLNTAMQAGTPPDIFQSWGGGVL
                     QQYAAAGLLKDITPDLQTGGWIDTFSPAALSLYSYQNKYYGVPWDIGAVGFWYNKDLF
                     AKAGITQLPVTWADFVNVIKRLKAAGIAPIALGEKDEWPGAFYWEYFAVRLGGKTAFD
                     NAYNHSGGSFTDPPFVEAGNYLQQLVALHPFQNGFLGASYNDHQAIMGNGQAAMELMG
                     QWAPANDASAATDKKGPNFGFFPFPTLPNEAGDPTDVLGGGNGFAIGKNAPPEAVDFV
                     RFLTSATNQRALAQKGALLPPVKAALDGVTNSNLQAVVQLVSNAPYYQLYYDQFMSPA
                     VGTTVNDQTARLFASTSTPTVVAQAINTSAAAGGG"
     gene            complement(2134..3177)
                     /locus_tag="VGT44_11170"
     CDS             complement(2134..3177)
                     /locus_tag="VGT44_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2581399.1"
                     /translation="MAQKSPISTQDITIYDVAREADVSYSTVSRVINNKGVSADKRER
                     VLRAMAELGYVANLQARSLAGGKSHIIGLLVHSLTVEYFDEIARGVDDELAALQYDLM
                     LYTTHRRKGRESAYVARVTRNLVDGLLLVLPRNAEAYLEALRHRHFPHVLVDHQGLGF
                     DVPSVGATNQKGGYDGTSYLIKLGHRRIGFITGDMALGCARERLSGYQQALSDAGLPA
                     DPGLIREGNFFQPRGFTCANELLDLAEPPTAIFASNDVSAFGVMEAVRSRGLRIPEDI
                     SVLGFDDISQAGQVNPSLTTVRQPLAEMGRAAARLLFTYISQPDAAIKQIVLPTELIV
                     RQSCQSPSVSSLT"
     gene            3256..3528
                     /locus_tag="VGT44_11175"
     CDS             3256..3528
                     /locus_tag="VGT44_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581400.1"
                     /translation="MDKQQAMLQNGQGDGKSARAMASIAPTINSREKRTNEGRVDACH
                     RPGLKALARHHTFLLPYVLAVQRSCGVWATRLIWYTDRSTTLLALL"
     gene            complement(3502..4707)
                     /locus_tag="VGT44_11180"
     CDS             complement(3502..4707)
                     /locus_tag="VGT44_11180"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEV2581401.1"
                     /translation="MSIDEPKMQFPAPSAVENPELIAYRDEFPILGRKTYLNSCSLGA
                     LSKRSMENMAQFMEMWNEWGAHAWYEIWMGEIARVREQFATIIGAQVHEVAIAPNVST
                     ALSSIASAFDYTKRNKVLLADRDFPTLAYQWLVKERLGVECQFVESPDQIYTPPELFG
                     EKIDDRTALIATSRVFFTSGYIQDIRKVADIAHKNGAYLLIDDYQGTGQIPLNVVTQD
                     IDMLVTGTLKWLMGGPGLALVYIREGLIPHLQPTITGWFGHAEQFKFKTREFAFRPDA
                     ARIELGTPAMAPIYAANGGLDIVREISVERICERTRFLTNDLIARAREHGWTVRAPIE
                     PERRSSIVMLEFDQPEDIVNELVARNIITDSRPGLLRISPYFYNTIEENAIVVDAIEE
                     ILQQRKKGS"
     gene            complement(4768..5943)
                     /locus_tag="VGT44_11185"
     CDS             complement(4768..5943)
                     /locus_tag="VGT44_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEV2581402.1"
                     /translation="MNNHPALQHGPIYLDYNATTPVDPAVVEAMLPYLASHFGNPSSS
                     HAYGHIAHDAVDIARAQVARLLGCSSSEITFTGGGSEGDNLAIRGAALALREQGNHII
                     TQATEHPAVLNTCRALERLHGFRVTYVPVDSAGRVNPADIEAAIDASTVLVTIMHANN
                     ETGTLQPIRDIAKIAHEHGVLMHSDAAQSVGKITTHVDELGVDLLSIAGHKLYAPKGI
                     GALYARRGLQLEPVIYGGGQEAGRRAGTENVPYMVALGTACELAREQLEASQLRLRSL
                     RDRLHNRLEELLPGRVHLNGHLTERLPNTLNVSIDGIAGEDVLAATPELAASTGSACH
                     EGNTDPSPVLIAMGYSHERALGALRLTLGRWTTEDEIERAAILLAKTVDSLLEKRYN"
     gene            complement(5940..6752)
                     /locus_tag="VGT44_11190"
     CDS             complement(5940..6752)
                     /locus_tag="VGT44_11190"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2581403.1"
                     /translation="MNAQPRKPDFLGEQFAARFQDQSVVDRYHLRATYPPETFRLLAD
                     LVVDEPRAVLDAGCGTGDVARHLLEYAERIDAVDISLPMLEKGKTLPGGDSPKIRWLH
                     GKIEEVELEPPYALITAGQSLHWMNWDLVLPRFQHILTPGGSLAILDVETMPTTWDDA
                     LIQIVKRYSSNKAYQPYALFDELERRQLFQKIGDQFTAPVTFEQSIQDRIEAMHAQSS
                     LSRDGMTADKIEGFHQEIETLLSPYTHHGKITLQVMGHLVWGRPLNPAKDIV"
     gene            complement(6749..8248)
                     /locus_tag="VGT44_11195"
     CDS             complement(6749..8248)
                     /locus_tag="VGT44_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV2581404.1"
                     /translation="MHDHERLDEAQQPSATGDMTPGDFRAYAHQAVDWMADYLENIEA
                     YPVLAQTAPGDIQDWLPSSPPRQPESMETIFADIDRVIMPGITHWNSPGFLAYFGITG
                     SGPGILGEMISGALNVNAMLWRTSPAATELEQVTLDWLRQMLGLPQPMFGVINDTASS
                     GILYALAAAREALPLHIRQHGMSGRPDVARLRFYASQEAHSSVEKAGIVLGIGQEGLR
                     KIGVDDAFRMDTIELEQAIQEDITQGWRPFAVVATVGTTSTTSIDPVPQIADICERYG
                     LWLHVDGAYGGSAAVDPTMRWVLAGCDRAESLIVNPHKWLFTPIDCSVFYTSRPGIVK
                     NAFSLVPEYLRNTQGGDDEVVNLMDYGTSLGRRFRSLKLWMIMRYFGQEGLAARIHEH
                     VRLGQLFASWVDESPTFERMAPAPFSTVCFRARPQGMHDEKQLETLNERILTRLNAGG
                     RFFLSHTKLRGQFTIRAAIGNLRTSERHIHELWDALQATLSTELREVEA"
BASE COUNT         1954 a         2379 c         2431 g         2020 t
ORIGIN      
        1 agttggagga ctagcgagag aacagcgatc aggacattgt tccttaatga ctgtagaaag
       61 atggggtcct cgaacagcgc ctggtagttt ttgaatccga tgtaatcggt gagatctccg
      121 agaccgctcc atcgatacag actatagtga gcagcttgta tgacaggaaa cacaacaaaa
      181 agaaaaaata gcaacatgct ggggagcagg aagatgataa tggttacatc tctgctccat
      241 cgacgacgga ggcgctgagt aaagattcct tgagttggta aagcccgttg aatagtaaac
      301 cccactgata ctgcgctccc ttctctccca tccaatggat gagatgatgt ctttgcagta
      361 atctgctaac cttcaagaga ccggtagggg cgaaccttta tggtcgcccg ccgtacatgt
      421 tccgatgtgt gcggcctcca cgggcgggcg actataaagg ttcgccccga ccggtctctt
      481 gcatctagtc tagagggaaa cccgcgacag atttccctcc cgactacctg ctgaatcaac
      541 ccccgccggc agccgccgat gtgttgatag cctgggctac taccgtaggc gtcgaggttg
      601 acgcgaagag tctcgcagtc tgatcgttga cagtggtacc aaccgcaggc gacatgaact
      661 ggtcatagta gagttgataa tagggcgcat tcgagacaag ctgcacgacc gcctgtaagt
      721 ttgaattggt gacaccatca agcgcagcct tgaccggagg caagagagcg cctttttgtg
      781 caagggcgcg ctggttagtg gcgcttgtca ggaatctaac gaaatcgacc gcttctggag
      841 gagcattttt gccgatggcg aatccgttgc cgcctcctaa gacgtcagtc gggtcgcccg
      901 cttcgttcgg cagcgtcggg aacgggaaga agccgaaatt tggccccttc ttatccgttg
      961 cagcgctggc atcgttggct ggcgcccatt ggcccatcaa ttccatggct gcctgcccat
     1021 tgcccataat ggcctggtgg tcattatagc tggcccccag aaagccattt tggaaggggt
     1081 ggagggcgac gagctgctgg agatagtttc ctgcctcaac aaatggcggg tcagtgaacg
     1141 accccccgct atggttataa gcgttatcga acgccgtttt gccaccgaga cgtacagcga
     1201 aatactccca gtagaaagcg cccggccatt cgtctttctc gccaagcgca attggcgcga
     1261 tacctgccgc tttgagacgc ttgatgacgt tcacgaagtc agcccaggtc acgggaagct
     1321 gtgtgatacc tgctttcgcg aagaggtctt tattgtacca gaagccgacc gctccgatat
     1381 cccaggggac accgtagtat ttgttctggt agctatacag ggaaagggca gcaggactga
     1441 aggtgtcaat ccacccgccg gtctgtaaat ccggggtgat atctttcagt aacccggcag
     1501 ctgcatactg ctgtaagacg ccacctcccc aactctggaa gatatcgggc ggggtgccgg
     1561 cctgcattgc cgtattcaac ttggttttaa agggatcatt cccgatgacg gtaatgttga
     1621 tctgcacgtt gggatgcgac tgcatgtact cgtaggcaag gttcttccac ccggataaaa
     1681 gtggatcagc cgtgttgata tgccaccagt taatggtgac cttgccggac ccactcgaac
     1741 cattgccgcc gctcccacag gcgaccatga gaatggccat gatcgccagc aaagacaaaa
     1801 gcacgcgatt acgtttcatg gttgactgtg ctccttgaaa ggtgaactgc aacgttgcac
     1861 acgacgggtc gatggatcac aggaaatcgc tctggcgtcc agtcagtcag aatgggtata
     1921 attactatcg ggttctggcg actttgcacc tcgatatgtt tgacagcgca tcaggtcgga
     1981 gtcgccgttt acttttgctc ctttcccccg atatcagtct ctgggaaaca ccgcttcgca
     2041 ccgtgtatcc atcttttctg gggaaaccag ctccgtttgt ctggtcacta cggaacactt
     2101 ttacatggga ctgtccacta tgtaacgctt acgctaggta agcgatgaca ctgacggtga
     2161 ctgacacgac tgccggacga tcaactcggt gggtaagacg atctgcttga tggcggcatc
     2221 aggctggctg atgtaggtaa agagcagcct ggcggcagct cgtcccattt cggccagggg
     2281 ttggcgcacc gttgtgagcg atgggttcac ctgtcccgcc tgggagatgt catcgaagcc
     2341 cagaacagaa atatcttctg ggatgcgcag gccgcgactg cgaactgcct ccatcacgcc
     2401 gaaggcagaa acatcattgg aagcgaagat cgctgtgggc ggttcggcaa gatcgagcag
     2461 ctcatttgcg caagtaaagc ctcgtggttg gaagaaattc ccctcgcgaa tcaggccagg
     2521 atcggcaggg aggccagcat cgctgagtgc ctgctggtag ccggacagtc gctcacgcgc
     2581 gcaccccagt gccatgtctc cggtaatgaa gccaattcgg cgatgcccaa gcttgatgag
     2641 atagctggtg ccatcgtatc cccccttctg attcgtggct ccgaccgatg gcacatcaaa
     2701 gcctaaaccc tggtgatcga ccagaacgtg gggaaaatgg cggtggcgca acgcctcaag
     2761 gtacgcctcg gcattgcggg gtaagacgag caagaggcca tccaccaggt tgcgtgttac
     2821 cctggcgaca tacgccgact cgcgcccttt gcgccggtgg gtggtataga gcatcagatc
     2881 gtactggagc gccgctaatt cgtcatcaac gccgcgcgcg atctcatcga agtactcaac
     2941 tgtgagacta tgcacaagca ggccgatgat atgcgacttc ccccctgcca ggctgcgagc
     3001 ttgcaagttt gccacatatc ccagctcagc catcgcgcgc agcactcgct cacgtttatc
     3061 agcgctcaca cctttattat tgatcactcg cgagacggtg gaataggaca catcggcttc
     3121 acgtgccacg tcgtaaatag tgatgtcttg cgtcgaaatt ggagattttt gcgccatcag
     3181 ttgctcctcg tcttgcaagc gcttgcaaaa tactgatacg agcatacctg atgtttggcg
     3241 caattgtcaa ggattttgga taaacagcag gccatgttgc aaaatggcca gggcgatggc
     3301 aagtcagcca gggcgatggc aagcatcgct cctactatca attcgcgtga gaagcgaacg
     3361 aacgagggta gggtcgatgc ttgccatcgc cctggcttga aagcgcttgc acgacaccac
     3421 acgtttctac taccttatgt cctcgctgtg cagcgttcct gtggagtgtg ggctacccgg
     3481 ttgatctggt acacagaccg gtcaactacc cttcttgcgc tgctgtaata tctcttcaat
     3541 ggcatcaacc actattgcat tctcttcgat agtgttgtaa aagtaaggcg agatgcgcag
     3601 caggccaggg cgtgagtcgg taatgatatt gcgggcgacg agttcgttta caatgtcctc
     3661 cggctggtca aattctagca tcacgattga gctgcggcgt tccggttcga taggcgcgcg
     3721 tactgtccag ccgtgttcgc gggcgcgggc gatcaagtca ttggtcagga acctcgtgcg
     3781 ctcgcatatg cgttccacgc tgatctcgcg cacgatgtcc agccctccgt tggctgcata
     3841 aataggagcc attgccggtg tgcctagctc gatgcgagcg gcgtcaggcc ggaacgcaaa
     3901 ctcgcgcgtc ttaaacttga attgttctgc atgtccgaac cagccggtga tagtcggctg
     3961 taagtgggga atgagtccct cgcgaatata gacgagcgcc agtcctggtc cgcccatgag
     4021 ccatttgagt gtgccggtga ccagcatatc gatatcctgg gtcacgacat tcaagggtat
     4081 ttgccctgtc ccctggtagt cgtcgatgag tagatacgcg ccgtttttgt gggcgatatc
     4141 ggcgaccttg cgaatatcct gaatgtagcc gcttgtaaag aacacccgcg aggtagcgat
     4201 taaggctgtc cggtcgtcga tcttctcccc aaacagttct ggtggagtgt aaatctggtc
     4261 ggggctttcg acaaactggc actcaacccc caggcgttct ttgaccagcc actgatacgc
     4321 cagcgtggga aaatccctat cggccagcag tactttatta cgcttggtat aatcaaaggc
     4381 agaagctatg ctactgagcg ccgtagacac attcggcgca atcgccactt catgcacctg
     4441 tgcgccgatg atcgtagcaa attgctcgcg cactctggcg atttccccca tccagatttc
     4501 gtaccaggcg tgcgcgcccc actcgttcca catctccatg aactgagcca tgttctccat
     4561 agagcgttta gagagcgccc caagcgagca gctattcagg taggtcttac gccccagaat
     4621 gggaaactcg tcccggtagg caatcagttc agggttctca acggctgagg gcgcaggaaa
     4681 ctgcatcttt ggctcatcga tagacatgtg cgctcactcc tttacaacga tggttagcgt
     4741 aagtctttca gaatggcatt gtattcttta attgtatcgc ttttccaaaa gagaatctac
     4801 ggtttttgcc agcaaaattg ccgctcgctc gatttcatcc tctgttgtcc agcgccccaa
     4861 agtcagccgc agcgccccca gcgctcgttc gtggctgtag cccatagcta tcaacaccgg
     4921 cgaggggtcg gtgttgccct cgtggcaggc cgatccggtc gatgcggcca gttccggcgt
     4981 cgcagccagc acatcttcgc cagcgatgcc gtcgatgctt acgttgagcg tattgggcaa
     5041 tcgttcggtc agatgaccat tgagatgtac ccgcccagga agcaattcct caagccggtt
     5101 atgcagcctg tctcgcaaag aacgcagacg gagctggctg gcctctaact gctcgcgcgc
     5161 caattcacag gctgtcccca gcgcaaccat atagggtacg ttctctgttc ccgcccggcg
     5221 gccggcctcc tgcccgcctc catagataac cggctcaagt tgcaagccgc gccgcgcata
     5281 caaagccccg atacccttgg gtgcgtagag cttatgcccg gcaatgctca gcaggtcaac
     5341 acccagttca tccacgtgag tcgtgatttt gccgaccgac tgcgctgcgt cgctatgcat
     5401 cagtacgcca tgttcatgcg cgatcttcgc tatatcgcga atgggctgta gcgtgccggt
     5461 ctcattattc gcgtgcatga tcgtgaccag cacagttgaa gcatcgatgg ctgcctcaat
     5521 atcagccgga ttgacgcgtc cagcgctatc gacaggcaca taagtaacac gaaatccgtg
     5581 caggcgttcg agcgcgcgac aggtgttgag aacggcagga tgttccgtcg cctgcgtgat
     5641 gatatggttg ccctgctctc tgagcgccag cgccgcccca cgaatcgcga ggttatctcc
     5701 ctcggaaccg ccgccggtga aggtgatctc cgacgacgaa catcccaaca gtcgtgccac
     5761 ctgggcgcgg gcgatatcga cggcatcatg cgcgatatgc ccgtaggcgt gcgagctgga
     5821 tgggttgccg aagtgggaag cgagataggg cagcatggct tctacgaccg ccgggtcaac
     5881 tggcgttgtg gcattataat caaggtagat tgggccgtgc tgtaaggccg ggtgattgtt
     5941 catacaatat cctttgctgg gttcaatggt ctgccccaca cgagatgtcc catcacctgc
     6001 aaggtaatct taccatgatg cgtatacggt gagagcagcg tttcgatttc ctggtgaaag
     6061 ccttctatct tgtcggcggt cataccatct cgtgaaagac tgctctgtgc atgcatcgcc
     6121 tcgatccggt cttgtatgga ttgttcaaac gtcaccgggg ccgtgaattg gtcgccgatt
     6181 ttctggaaaa gttggcgcct ttcaagctca tcgaataaag cgtatggttg atacgcttta
     6241 ttcgatgagt agcgtttgac gatctggatg agcgcatcat cccatgtcgt aggcatagtt
     6301 tctacatcca ggatggccag gcttccgcca ggtgtgagta tgtgctgaaa gcgcggtaaa
     6361 accaagtccc aattcatcca gtgcaggctc tgcccggcag ttatcaaggc gtagggtggc
     6421 tcaagttcaa cttcctcaat cttaccgtgc agccagcgaa tttttggaga atcgccgcct
     6481 ggcaacgtct ttcctttttc tagcattggc agggatatgt ctacagcatc gatgcgctcc
     6541 gcatattcaa gcaggtgccg cgcaacgtca cctgtgccgc agcctgcatc caggactgcg
     6601 cgcggctcgt caacgaccag atcggcaagc aggcggaaag tctcaggtgg ataggtcgcg
     6661 cgcaggtgat agcggtcaac gacgctctga tcttgaaagc gagcggcgaa ttgttcgccg
     6721 agaaagtcgg gtttcctggg ttgagcgttc acgcttctac ctcgcgtaac tcagttgaca
     6781 gggtggcctg taatgcgtcc cacaactcgt gtatatgacg ttcggatgtg cgcaggttgc
     6841 cgattgcggc gcgtatggta aattgaccac gcagctttgt atgcgaaagg aaaaagcggc
     6901 ctcccgcgtt gagacgggtc aagatgcgtt cgttcaacgt ttctagctgc ttttcgtcat
     6961 gcattccctg ggggcgggcg cggaagcaga ccgtgctgaa cggcgcgggt gccatacgct
     7021 cgaaagtggg tgattcatct acccacgagg cgaaaagctg gccgaggcgc acgtgttcgt
     7081 gaatgcgcgc cgccagtcct tcctggccga aatagcgcat aatcatccag agcttgagcg
     7141 agcgaaagcg gcggcccagc gaggtgccat aatccatcag gttgaccact tcatcatcgc
     7201 ctccctgcgt gttgcgcaga tattcgggaa ccaggctaaa cgcatttttg acgatgccgg
     7261 gtctgctcgt atagaagacg ctgcaatcga tgggagtaaa gagccatttg tgcgggttga
     7321 caatcagact ttcggcgcga tcgcagcctg ccagcaccca ccgcatcgtg gggtcaacgg
     7381 cggcagaacc gccgtatgcg ccatcgacat gcagccacag gccgtaacgc tcgcaaatgt
     7441 cggcgatctg tggtaccggg tctatgctgg tcgtcgaagt agtgccgacg gtcgcgacga
     7501 cggcgaaggg acgccagccc tgtgtgatgt cttcctggat ggcttgctct agctctatcg
     7561 tatccatgcg aaaggcgtca tccacaccga tcttacgcag cccttcctgg ccaatgccca
     7621 gcacaatgcc cgccttttcc accgatgagt gcgcttcctg cgaggcataa aaacgcaatc
     7681 gagctacatc cgggcggcct gacatgccat gctgcctgat atgcaacggc agcgcctcgc
     7741 gagccgccgc cagggcatac agtatacctg acgaggccgt atcattgatc acgccaaaca
     7801 tgggttgagg cagaccgagc atctggcgca gccaatcaag tgtgacctgt tccagctccg
     7861 tcgccgctgg agaagtgcgc cagagcatgg cattgacgtt gagcgcgccg gagatcatct
     7921 ctcccagaat tcctgggccg gagccggtga ttccgaaata ggctagaaag cccggcgaat
     7981 tccagtgcgt gatgcccggc ataatcaccc ggtcgatatc tgcaaagatc gtttccatcg
     8041 actcaggttg gcgcggcggc gagcttggaa gccagtcctg gatatcgccc ggcgcggtct
     8101 gcgccagcac gggataggct tcgatatttt caagataatc cgccatccaa tccaccgcct
     8161 ggtgggcata cgcgcggaaa tcccctgggg tcatatcgcc ggttgcagat ggctgctgcg
     8221 cctcgtcaag acgctcgtga tcgtgcatga aggtttcctc agctaaacta aatcatgtac
     8281 tactattgaa gtgatcgggc aattgtatta tgccggtgat gtagtcagta tctcacagtg
     8341 taagattgca aacaagcata ttcgattgat gaattgctct caacagacgt gataactcag
     8401 gcaagtggag tgaatatcaa tgtaatgctg agaattgtat tgggcagcca tttgttcatc
     8461 gcaaaaaccc tatccctttt ctgtgcttgt aaatactgtg tgcttctatg tagagaaaaa
     8521 gccatgttcc gatatagttt cctttggcgg cctgctctca ttttactccc tctgattgtt
     8581 gcctcgatgt agacagtgga taatatgccg aacataaaat accaataata tagcaaaaat
     8641 caccaaaata tcttgacaaa ataaaaataa tatggtaatt actatcacag tgatatatca
     8701 ttatgatagt aaaaaggagc aatggtcaca gcgatgtgaa agggaggacg atgtacgagt
     8761 tcattgtgct ggggcagttg atgt
//
LOCUS       DASZAK010000639         3267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_50846, whole genome shotgun sequence.
ACCESSION   DASZAK010000639 DASZAK010000000
VERSION     DASZAK010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50846"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..1688)
                     /locus_tag="VGT44_11200"
     CDS             complement(387..1688)
                     /locus_tag="VGT44_11200"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEV2581405.1"
                     /translation="MNTQDQKRVMVLNRILDGQVSTAEAAALLKLSKRQVQRILAAYR
                     KEGAATLVHGNRGRSPIHRIKEETRQQILRLAQTTYVGANQQHLRDLLEEREGIVVSR
                     ATVRRIVQPAGSPQRKPPQHRRRRKRYAQEGMLVQIDGSPHAWLEERGPRLHLLAAID
                     DATGKVLAAIFREQEDRVGYFQLVEQLVTRYGRPLAFYHDRHEIFPKQRAVSEADSLE
                     EQLAGRKTPSQFGRLLEELEITSIAAHSPQAKGRVERLFGTLQDRLVIELRLAGATTR
                     EQANAHLADYLPRFNAQFAVPAAQEGSAYRAVAATFKPEEVYCFKYERVVAADNTVQF
                     GQQRLQVLAGQVRRSYAHVTVEVHERFDGSLAIFYAGVCLASTPASLEAPQLRARGKR
                     LRLSLMPASTSTPAEPPAPAEVQTHKPPASSKPRADHPWRK"
     gene            1903..2730
                     /locus_tag="VGT44_11205"
     CDS             1903..2730
                     /locus_tag="VGT44_11205"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEV2581406.1"
                     /translation="MNTPLQSTGMMSDELALSDLDAPVEEHGEAEHIHLPGPSYWPIF
                     ISVALLITFVGLLFINSTPVLVIIGAVLALIGIVGWGVENPMAPRAAAVKPGEFSTSF
                     AEAAISGKPTPLAQAALRDAEDVVEQTVTIASTAWSAHPVKVFVEREGVVLSLYGKVE
                     LEAQKQEVEKALRKMPGVIDVMNFLVAEDELLNAVNKTIDELKASGKLEGATNIHALV
                     ENYIVSLYGEVPKPEMKYLLEREITTIPGARVVINHIGLNKDIPGEMGKTSNRIARI"
     gene            complement(2791..3045)
                     /locus_tag="VGT44_11210"
     CDS             complement(2791..3045)
                     /locus_tag="VGT44_11210"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HEV2581407.1"
                     /translation="MSGNRGCIFNSLLFLIIVFVPVLGHIIATVMVFEDNHSAAGTVL
                     WLALIWLVPFLGSFLYLLFGQRRRRVMFGQPNYAYQYQQR"
BASE COUNT          742 a          899 c          909 g          717 t
ORIGIN      
        1 ccattcatgt tgtggcatag tcacaatagc ttcccagcta tcagttatac gagcctgata
       61 tggctgggtt gcgcttctaa tgatttacgg gcatcaaaca taacttttat atccatgaaa
      121 atatagtagc acaggtttta aattttgcaa ggctgagtcg cgcatgatgt caacgtccag
      181 tgaaaatgtc acctgaactg ttcagtgagt ttgtcatcgt caatcaggaa gcccaagcgt
      241 accgcgtcag ctttcccacg tcaagccaag ttgcgcgagt acacgcaatg cgcttgacgt
      301 gggaaagctg acgcggtaag ggcttcgtac ctgattgaca gccaaaaaaa tgtgcctgtt
      361 tttttgctga agcaaagcca ctcccgtcac ttgcgccacg gatgatcggc ccgtggtttg
      421 gaagaggcag gcggcttgtg cgtctggacc tctgccggag ccggtggttc tgccggggtc
      481 gaggtggaag caggcataag tgacaggcgt aagcgcttgc cccgcgcacg cagttgaggc
      541 gcttccagcg aagccggcgt cgacgccaga cagacccccg cgtagaaaat ggcgagcgag
      601 ccatcgaagc gctcatgcac ctctaccgtg acatgtgcat agctgcgccg cacctggccc
      661 gccagcacct gaagacgctg ctggccgaac tgcacggtat tgtctgccgc caccacgcgc
      721 tcatacttga aacaatacac ctcctctggc ttgaacgtgg ccgccaccgc acgatacgcg
      781 ctcccttctt gcgccgcagg caccgcaaac tgcgcattga agcgcggcag gtagtccgcc
      841 agatgcgcat tggcctgctc gcgcgtggtg gctcccgcca ggcgcaactc gatcaccagc
      901 cgatcttgca atgtgccaaa gagtcgctcc acgcgcccct tggcctgggg cgaatgggcc
      961 gcaatcgaag tgatctccaa ctcctcgagt aagcgcccga actgactggg ggtcttccga
     1021 cccgccaact gctcttccag cgagtcggcc tcgctgaccg cacgctgttt ggggaagatc
     1081 tcatgccgat catgatagaa ggccagcggt cgcccatagc gtgtgaccag ctgctccact
     1141 aactggaagt accccacacg atcttcctgt tcccgaaaga tggccgccag cactttgccg
     1201 gtggcatcat caatggctgc caacaggtgc aagcgcggcc cgcgctcttc cagccaggca
     1261 tgcggactgc catcgatctg caccagcatg ccttcttgcg cgtaccgctt tcggcgacga
     1321 cgatgctggg gcggcttgcg ctgcggcgag cctgctggtt gcacaatacg ccgcacggtc
     1381 gctcgtgaga ccacgatccc ttcacgctct tccaacaagt cgcgcaggtg ctgctggttg
     1441 gctcccacat acgtcgtctg ggccagccgc agaatctgct ggcgcgtctc ttccttgatc
     1501 cgatggatgg gggaccgtcc tcgattgcca tgaacaaggg tggcggctcc ttcctttcga
     1561 taggccgcca ggatgcgctg cacctgccgc ttcgagagct tgagcagggc agccgcttcg
     1621 gctgtactga cttgtccatc gagaatccga ttcaagacca tcactcgttt ctggtcttgc
     1681 gtgttcaata tcattgtctc ctgcatagtg acattttcac tgaacactta ccctatgaca
     1741 atatcactga tcgctaacaa atgcgagtac aggtcttgca agaacctggc attcctgcta
     1801 tgcttagtaa aagggtactg ccggaatgct ggcggtggca gtcggcatta gtggcagtat
     1861 acgaagcggt taaaaggcat tctcgaacga aagcgagaac ttatgaatac tcctcttcaa
     1921 agcacaggga tgatgagcga cgaacttgcc ttgtcggatc tcgacgcacc ggtagaagag
     1981 catggtgagg ccgagcatat ccacctgccg gggccgagct actggcctat cttcatcagt
     2041 gtggctctgc tgattacatt tgttggcttg ctgtttatta attccacacc cgtcttagtc
     2101 ataattggcg cagtgctggc gctgattggc attgtgggtt ggggggtcga aaacccgatg
     2161 gcgcccaggg cggcagcggt caagcccggc gaattttcca cctcgtttgc ggaagccgcg
     2221 atatcgggca agccgacgcc cctggcacag gctgcgctgc gggatgccga ggacgtggtt
     2281 gaacagacgg ttacgattgc ctcgacagcg tggagcgcgc acccggtcaa agtatttgtg
     2341 gagcgagagg gcgttgtcct ctcactgtat gggaaagtag aactggaagc gcagaagcaa
     2401 gaggttgaga aggcgctgcg caagatgcca ggggtgattg atgtgatgaa tttcctggtt
     2461 gctgaggacg aactgctaaa tgccgtcaat aagacgatcg acgagctaaa ggcatcgggg
     2521 aaactggaag gcgcgaccaa tattcacgcc ctggtagaga actacatcgt cagcctgtac
     2581 ggtgaggttc ccaagccgga gatgaagtac ctgctcgaac gcgaaatcac tactattcct
     2641 ggtgcgcgcg tagttatcaa ccacattggc ctgaataaag atattcctgg cgaaatgggt
     2701 aagacctcga accgtatcgc tcgcatttag cgctactgac agaaacaaca aagacgcccg
     2761 gcaccgcgtg atgccaggcg tctttttgtt ttatctctgc tggtattgat aggcgtagtt
     2821 cggctggccg aacatgacgc ggcggcggcg ttggccgaac agcaggtaca ggaacgatcc
     2881 caggaatggc acgagccaga tcaacgccag ccagagaacg gtgccagccg cagaatggtt
     2941 gtcctcaaag accatgactg ttgcgatgat atgccccagc acaggcacga acacgatgat
     3001 caggaacagc agcgaattga atatacatcc tcggttgccc gacatttttt tctcctcaaa
     3061 ctaacagcgc cggcccgatt tctgttcaaa acgagagctt gattgaccag cgccgtatta
     3121 cggctctcac tggtatatac gaagacgcac tggtgagcgt tgtaaggcat tagtaatatt
     3181 agaacaaatt tacttgacac atgttctaat taatggtaga ctttatgaaa atagaacaaa
     3241 tttactatat agggcgatat ttgcgct
//
LOCUS       DASZAK010000640         4604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_621395, whole genome shotgun sequence.
ACCESSION   DASZAK010000640 DASZAK010000000
VERSION     DASZAK010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4604
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621395"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="VGT44_11215"
     CDS             complement(<1..457)
                     /locus_tag="VGT44_11215"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEV2581408.1"
                     /translation="MLLNLLEYHWAEDIDEALILLSRTDTRTVPLAGGTYLLGHDDER
                     IQAVVDLRDLDLAYVREDARGVHIGAMTTLQAMADAPVLRDLAAGLLARAAQVSASSR
                     LIRNGATLGGTVALGAASQAADLLTALAAMEAEALARSGTKSQVDLGAGS"
     gene            complement(463..2190)
                     /gene="ade"
                     /locus_tag="VGT44_11220"
     CDS             complement(463..2190)
                     /gene="ade"
                     /locus_tag="VGT44_11220"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEV2581409.1"
                     /translation="MNDVQRRISVARGNSPADLVLRNSRLVNVCSGECYPADVAIADG
                     LVVGVSEPGAGYHGREERDLQGRWLAPGLIDGHMHIESTMLVLPEFARLVVPRGVTAV
                     MLDPHEFANVMGVAGIRFVLESARNLPLSAYIMLSSCVPASSFESPYRVLVADDLLPL
                     LGEEQVLGLAEMMNMPGVLQGDEQVLAKISATLAHGLVVDGHAPGLNERDLCAYASAG
                     VMSDHECTTFEEARLRLRLGMWLMIREGSAARNLEALLPLVQELHPPRVFFVTDDRDP
                     QDLTTRGHIDSMVRRAIELGLDPVEAIRLASYNTAQYFRLYQRGAIAPNFVADLVVLD
                     DLNTFKVESVYKDGRMVARQGALLADVPAVSYPGVTGTVHIGDVSEEMLRIPGKPGMV
                     EVVGIQPGQITTLHLREEAPVLDGAIVADPARDLLKLVVIERHHASGRVGLGLVKGFG
                     LRRGAIASSVAHDAHNLVIAGANDSDILQAARVLETMGGGFACVVDGEVRASVALPYG
                     GLVSPLPAAELVRQLRALDAAAAELGCTLEHPAMTLSFLSLSVIPSLKLTDQGLVDVE
                     TFTLLTLQG"
     gene            2572..>4604
                     /locus_tag="VGT44_11225"
     CDS             2572..>4604
                     /locus_tag="VGT44_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2581410.1"
                     /translation="MKTSEKARPNELLSHAREQRHWTQEEVATKIGAPDAKMVGRWER
                     GIILPTAHYRQQLMTLFGKSAQELGLVTPDQVPFWQIPYRRNSFFTGRAYLLDQLSTT
                     LTFDKSSALKLPLALSGLGGVGKTQLALEYAYRYSHMYHTVAWLQADSPEVLATSFAG
                     LAELLRLPERTNDDLEQNIRAVKNWLAKMSRWLLILDNVESLFTLESFLPSPLRGHIL
                     LTTRTQISGTAAQVLEVETMPIDEAALFLLRRAKIVAIDASPDDSSGGEFRQARAIAV
                     ELGGLPLALDQAGAYIEETACGLAPYLELLQIKRKELLQRRGPDLTNHPEPVATTWSL
                     SFDRLRQENPAAIDLLTLLAFLSPDAIAEEIILDGVNEAGSEPDLATHLTKLSSDPLL
                     FNQAIKDLRRFSLVRRQSKTRIITIHRLVQAMLLENLQPDEQRLWAGYTVRAICKVFP
                     DGNLETLPQGDRLLAQISVCERLIGQWQMRFPEAAQGLRRMAMYYLNRSLFEQSERIS
                     QQAIALQESLYGPEHLELAGTLSGLAMLYLYSYRYTEAEPLYKRIIAIRTALLGSEHH
                     ETGSSLADLAWLYTILERYDEAERLFHEAVYILQRHFGEDHLEVGSVLNNWAGLYMRQ
                     GNFAQAETMLERAIAISEQHLSDDHPDLAINRHNLAKLYSAQGKYQQAESLLLR"
BASE COUNT          975 a         1442 c         1317 g          870 t
ORIGIN      
        1 agctgcccgc gccgagatcg acctgggatt tcgtgccaga acgggccagc gcttcggcct
       61 ccattgccgc cagcgcggtc agcaaatcgg cagcctggga ggctgcgccg agcgctacgg
      121 tgccacccag cgtagcgccg ttgcggatca gccgcgagga cgcggacacc tgcgccgcgc
      181 gagccagcag gcccgccgcc aggtcgcgca gcacgggcgc atcggccatc gcctgcaagg
      241 tggtcatcgc gccaatatgc acgcccctgg cgtcctcgcg aacgtaagca agatcgagat
      301 cgcgcaggtc tacgacagcc tggatgcgtt cgtcgtcatg ccccagcaag taggtgccgc
      361 ccgccagggg aactgtcctt gtatccgtgc gtgagagcag gatcagcgct tcgtcgatat
      421 cttcagccca gtgatattcg agcaagttga gtaacacggt ttttaccctt gtaatgtcaa
      481 caacgtaaac gtctccacat cgaccaggcc ctggtcggtc agcttgagcg agggaatgac
      541 cgagagcgag aggaagctga gcgtcatggc gggatgttcg agcgtacagc ccagttcggc
      601 ggcggcggca tccagcgcac gcagttgccg caccagttcg gcggcgggca gcggcgagac
      661 cagcccgcca tacggcagcg ccacactggc gcgcacctcg ccatcgacaa cgcaggcaaa
      721 gccaccaccc atcgtctcca acacgcgcgc agcctggaga atatcgctat cattggcccc
      781 ggcaatcacc aggttatggg catcgtgcgc gacgctgctc gcgatagccc cccggcgcag
      841 accaaagccc ttgaccagcc ccaggccaac gcgcccgctg gcgtgatgcc gttcgatcac
      901 caccagcttg agcaagtcgc gcgcggggtc tgccacaatc gcgccgtcca gcacaggcgc
      961 ctcttcccgc aggtggagtg tggtaatctg acccggctgg atgcccacaa cctcgaccat
     1021 gcccggcttg cccggaatgc gcagcatctc ctctgataca tcgccgatat gcacggtgcc
     1081 ggtcacacct ggatatgaaa cagcggggac atcggccagc aacgcgcctt gtcgagcaac
     1141 catgcggcca tccttataga ccgattcgac cttgaaggtg tttagatcat ccagcacgac
     1201 caggtctgcg acaaaattgg gcgcgatagc gccgcgctgg tagaggcgga aatactgagc
     1261 ggtattgtag ctcgccaggc ggattgcctc gacggggtca aggccaagct caatggcgcg
     1321 gcgcaccatc gagtcgatat ggccgcgcgt ggtcaaatcc tgcgggtcgc gatcatcggt
     1381 gacgaagaag acgcgcgggg gatgcagttc ctgcacgagc ggcagcaatg cttcgaggtt
     1441 gcgcgcggct gacccctcgc ggatcatcag ccacatgccc aggcgcagac gcagacgggc
     1501 ctcttcgaac gtggtgcatt cgtggtcgga catcacgccc gcgctggcat aagcgcacag
     1561 atcgcgctcg ttcaggccgg gagcgtgtcc atccacaacg aggccatgcg caagcgttgc
     1621 agagatcttg gccaacacct gctcgtctcc ctgcaatacg cccggcatat tcatcatttc
     1681 ggccaggccc aacacctgct cctcccccag cagcggcagc agatcatccg ccaccaggac
     1741 gcggtagggg ctttcgaatg acgaggccgg tacgcaggac gagagcatga tgtaggcgct
     1801 caggggcagg ttgcgcgcgg attcgagaac gaagcgtatc ccggcgacgc ccatcacatt
     1861 cgcgaactcg tgtgggtcga gcatcacggc ggtcacgccg cgcggcacga cgagacgggc
     1921 gaattcgggc agcaccagca tggtgctttc gatatgcata tgcccatcga tcaaccctgg
     1981 ggcaagccag cgtccctgca agtcgcgctc ctcgcgaccg tgatagcctg cgcccggctc
     2041 gctgacaccg accaccaggc catcggctat agccacatcg gcgggatagc actcgccgct
     2101 gcacacgttc accagtcggc tattgcgcag cacaagatca gcaggcgaat tccctcgcgc
     2161 gaccgagatg cgtcgttgca catcgttcat tgagtcaatc tcctcccagt tagacaggta
     2221 gtttctgctc gattgcattc atttttcttg tcaaacatgg taacagaaac tcaggcaatc
     2281 gtcgagggga ttacctacca atatcctatc gcaattgcgt tttgtcacga gaggggatat
     2341 actctgcatt ggccatcgat cgtggaaact tctcagacac gccaaatagc ataaaggcgg
     2401 cttttgccct tatgctgcgc aatcaaatac ttacggtcta ccggcgctcc tgcacttttt
     2461 ccccccgtgc aggagcgccg gcaacccgat agctttaacc aggaatattt atagcatgta
     2521 tccaggttga ttgtacacgg cattctgggg gcaagctgtg gaaggaactc tatgaagaca
     2581 tctgaaaagg cgcgcccgaa tgaattattg agtcatgcgc gcgagcagcg ccactggact
     2641 caggaagaag tggcgactaa aatcggcgcg cccgatgcca aaatggtggg acgctgggag
     2701 cgcggcatca tcctccctac agcgcattat cgccagcagt tgatgacgct tttcggtaaa
     2761 agcgcgcagg aactgggact ggtaacgccg gaccaggttc cattctggca gattccctac
     2821 cggcgcaatt ccttcttcac aggacgcgcc tatcttctcg accagctctc taccactctc
     2881 accttcgaca aatcttctgc cctgaagctt cccttagcgt tgagcgggtt gggaggcgtg
     2941 ggcaaaaccc agctcgcgct tgagtacgcc tatcgttaca gccacatgta tcataccgtt
     3001 gcctggctac aggccgattc gccggaagta ctcgcaacct ccttcgcggg gttggccgag
     3061 ctgctcaggc tacccgaacg aacgaacgac gacctggagc agaacatccg ggcggtgaaa
     3121 aactggttgg caaagatgtc gcgctggctg ctcattctcg acaacgtcga atcgctcttc
     3181 acgctcgaat catttctgcc atctcctttg cggggtcata tcctcctgac tacgcgcacg
     3241 cagattagcg gtacagcagc ccaggtactc gaagtcgaga cgatgccgat cgatgaggcc
     3301 gcgctattcc tcttaaggcg cgcgaagatc gtggcgatcg atgccagtcc cgacgattcg
     3361 agcggcggcg agttccgaca ggcaagggcc atcgcggttg agcttggcgg gctgccactg
     3421 gcgctcgacc aggccggagc ctatatcgag gagaccgcct gcggcctggc tccatatctg
     3481 gaattgttgc aaatcaagcg aaaggagtta cttcaacgac gcggacctga tctaaccaat
     3541 catcctgaac cggtagcgac cacctggtcg ctctcgtttg atcgactccg ccaggagaac
     3601 ccggcggcga ttgacctgct gacgctgctt gctttcctct cgccagacgc catcgccgag
     3661 gaaatcattc tcgacggcgt aaacgaggcc ggttcagagc ctgatctggc aactcacctg
     3721 acgaaactgt ccagcgatcc actgctgttc aaccaggcca tcaaagacct gcgacgcttc
     3781 tcgctggtac gcaggcaatc gaaaacgcgc atcatcacga tccaccgtct tgtgcaggca
     3841 atgctgttag aaaacttgca gcccgatgag cagcgcctct gggctggata tactgtgcgg
     3901 gcaatttgca aggtttttcc tgacggcaat ctcgagacgc tgccgcaggg cgaccgcctg
     3961 ctggcacaga tcagcgtatg cgagcgcctg atagggcaat ggcagatgcg ctttccagag
     4021 gctgcccagg gattgcgccg catggcgatg tactatctca atcgctccct gttcgagcag
     4081 tccgagcgta tctctcaaca ggccatcgcg ctgcaagaat cgctctacgg ccccgaacac
     4141 cttgagcttg ccgggacgct ctccggcctg gcgatgctct atttatactc ctatcgctat
     4201 accgaggccg agccgctcta caaacgcatc atcgccatac gcaccgctct cctgggatcg
     4261 gaacatcacg aaacagggtc cagcctggcc gacctggcct ggctctatac catcctggag
     4321 cgatacgacg aggcagagcg tcttttccat gaagcggtat atatcctcca gcgtcatttt
     4381 ggcgaggatc atctggaagt cggatcggtg ttaaacaact gggccggcct ctacatgcga
     4441 cagggtaact ttgcccaggc ggaaaccatg ctggaacgcg ctatcgccat cagcgagcag
     4501 catctgagcg acgatcaccc cgacctggcg attaaccgtc acaacctggc caagctctac
     4561 tcggcgcagg ggaaatacca gcaggcggag tcgttgttgt tgcg
//
LOCUS       DASZAK010000641         3730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_122287, whole genome shotgun sequence.
ACCESSION   DASZAK010000641 DASZAK010000000
VERSION     DASZAK010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122287"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VGT44_11230"
     CDS             <1..483
                     /locus_tag="VGT44_11230"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2581411.1"
                     /translation="IFNELGDRVDAARVMNAQGALLLADGDYAGARELYERARALAHE
                     QEARQVEGRALRGLGDAACATRHFSEAERQYAAAAELAQMLDTPAEHSAVLYRQGHLL
                     AAQAHYQQALETWAEALFQDRRADHPERLEHEARIADFVAQHHLEEDYSALRSQYKLV
                     "
     gene            603..2066
                     /locus_tag="VGT44_11235"
     CDS             603..2066
                     /locus_tag="VGT44_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581412.1"
                     /translation="MATTVQPPAPPKTSGAGKAFTWIAGILCFGLLGYLLLQIVHQVV
                     VPPPPQRLILIQDVPLPSGLPTPAQEQSLIPDAAQVGTTLNPGVAIDFDAFDFQALDV
                     PTHLLFVAHSGPSPDFLAADKNVDFNATRDAPFDGNVIVFDTQQQKVVARINIPQVAG
                     VVDAPDLGKVFVGSDAATNNDQSIVYAIDVHTLKATPIPLANLESPDALSYDAMNKRI
                     YASDPGAPADPAKTMNVDRNNENIAVIDAVNDKLLKYINLGNLPLLPGEKVPAALLSP
                     TNSAIPRYGHDVGHNVFDEGLQRLFVTSTVLPNGDDPNPFVLPPTGTGEFFEIDPANS
                     ANPIVKEIDLPATCSTPHGMNIDEAQQVAFIACTDVDSASNLFPNLVLVNLNTMTVIP
                     TLPNATRLEDGPDIVRLDQNSTLKIDILFVACKAGITIFDITPGHFQKLGDVILGKET
                     HTIAIDEASQYVYLPLTIGGRPVLRVARYNPNGQSSF"
     gene            complement(2108..>3730)
                     /locus_tag="VGT44_11240"
     CDS             complement(2108..>3730)
                     /locus_tag="VGT44_11240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2581413.1"
                     /translation="GSLQAQFAPIVGFLVTIGTAAVIGVGGYVAAGARFNLGFFTVQA
                     ESVDIGTLILFLIFLNLLYQPMRDLSKLTALYGTAASGAERIQEVLDQAPEVLDYQSL
                     YNGPTRLKGDIRFENAVFGYTPEQAVLKGINLHIPAGKKVALVGLSGGGKTTLVKLIP
                     RFYEIQQGSVKVDGVDNRAYPLTVLRQNVSMVLQDSVLFEGTVAENIAIGKPGAPLYE
                     VIEAAKKANIHETIIRDLGGYDGAIREQGKNLSGGQRQRMAIARAILRDAPILILDEP
                     TAALDVESEAEVMHALEKLVVGRTVIMISHRLSTLGNVEEIIVLKNGQIAEQGNFQEL
                     KRRGGVFAALLAEQNRYNLEKAAEKSMIRSAFVPLDVYDPRQGQQVPFPPPRTPIFPP
                     TPVPPAGQNWPLPPTPMGQPSRLQSPIYASASPDSIGWQQQQQRVAMPVAPQRVRVMI
                     EVDGKVVGERVLNKPSLTVGRLSGNDVQVPSQRVSRLHAKIHEENGTWVIEDAESLNG
                     LIFRGQRIDRLTLSNGDRVYVAPTVVLQFVTP"
BASE COUNT          810 a         1104 c         1029 g          787 t
ORIGIN      
        1 atcttcaatg agctaggaga ccgcgtcgat gccgcgcgcg tcatgaatgc tcagggcgcg
       61 ctcctgctgg ccgatgggga ctacgcaggg gcgagggaac tgtacgaacg ggcgcgcgcg
      121 ctggcacacg agcaggaagc ccgccaggtg gagggtcgcg ccctgcgcgg tctgggcgac
      181 gccgcctgcg ccacacgcca tttcagcgag gccgaacgcc agtatgccgc agcagcagaa
      241 cttgcccaga tgcttgatac gcccgccgaa cactcagccg tgctgtatcg ccagggtcat
      301 ttgctcgccg cgcaagcgca ctaccaacaa gccctggaga cctgggccga agccctcttc
      361 caggaccgcc gcgccgatca tcccgaacgc ctggaacacg aggccagaat cgccgacttc
      421 gtcgcgcagc atcacctgga agaggactat tccgctttgc gcagccagta caagctggtc
      481 tgatatcttt cctcaggtaa gcaagtctgc tataatgctc acattaactt cagttcgctt
      541 gaaccttgat ggactactac actgcacgga gggagagaga tcatgattgg gaggttttac
      601 ctatggctac aaccgttcag ccaccagcgc cgcctaagac ttcaggcgct ggaaaagcat
      661 ttacctggat agcaggcatc ctctgttttg gtttactggg atacctgctg ctccagatcg
      721 ttcatcaagt cgtggtaccc cctccgcctc aacggctcat actcatccag gatgttccac
      781 ttcccagcgg tcttcctaca cccgcccagg aacaatcgtt gattccagac gctgcccagg
      841 ttggaaccac gctgaatccg ggcgtggcga tagacttcga tgcgtttgac tttcaggcgc
      901 tcgacgtacc cacacacctg ctgtttgtcg cgcacagcgg gccaagccct gacttcctgg
      961 cggcggacaa aaacgtggac tttaatgcaa ctcgtgatgc gccttttgat ggaaatgtga
     1021 ttgtctttga cacccagcag caaaaagtcg ttgcgcgtat caatatccca caggttgccg
     1081 gagttgtgga tgcccccgat ctgggtaaag ttttcgtcgg cagtgatgct gctaccaata
     1141 acgatcaaag catcgtgtac gctatcgatg tacacacctt gaaagccacg cctatcccgc
     1201 ttgccaacct tgaaagcccc gacgcccttt catacgacgc catgaataaa aggatttacg
     1261 cttccgaccc tggcgctccg gcagacccgg ccaaaacgat gaacgtggac aggaataatg
     1321 agaatatcgc agtcattgat gccgtcaatg acaaactcct caaatatatc aatctgggca
     1381 atctgccgct tctgccggga gaaaaagtcc ctgctgccct gcttagcccc acaaatagcg
     1441 ccattccgcg ctacggccat gatgtcgggc ataatgtctt cgacgaaggc ttgcagcgtc
     1501 tctttgtcac ctctacggtc ctgccgaacg gcgatgaccc gaatcctttc gttctgccgc
     1561 caacggggac aggcgagttc tttgaaatcg atcccgcgaa ttccgctaac ccgattgtca
     1621 aggaaatcga cctgccggca acgtgcagca caccccacgg catgaatatc gatgaagcgc
     1681 agcaggtggc ctttatcgcg tgcaccgatg tcgattcggc cagcaacctt ttccccaatc
     1741 tcgtgctggt gaatctcaac accatgacgg tcattcccac gcttcccaat gccactcgct
     1801 tagaggacgg ccccgatatc gtcaggttag atcagaacag cactttgaag atcgatatcc
     1861 tgtttgtggc ctgtaaggct ggcattacca tctttgacat cacgccgggt cattttcaga
     1921 aactcggtga cgtaatcctg ggcaaagaga cccataccat cgccatcgat gaggcgtcgc
     1981 aatacgtgta cctgcctctt acgattgggg gaagacccgt tttaagggtc gccaggtaca
     2041 atcccaacgg acagtcaagc ttttagacat gccatcagcc gtagggacag ggtgttgggg
     2101 taacgcatta cggtgtgacg aattgcagca ccacggtggg ggcgacgtaa acgcgatcgc
     2161 cgttgctcag cgtgagacgg tcaatgcgct ggccgcggaa gatcaggccg ttgaggcttt
     2221 ccgcatcctc aatgacccac gtgccgtttt cctcgtgtat cttagcgtgc aatcgcgaga
     2281 cgcgctgcga cggcacctgc acgtcattcc ccgatagacg cccaactgtg agggagggct
     2341 tgttcaaaac acgttcgccg acgactttgc catcaacttc gatcattacg cgcactcttt
     2401 gaggagcaac gggcatagct actcgctgct gttgctgctg ccaaccaata ctatccgggc
     2461 tagcagaagc ataaatgggt gactgtagcc gtgaaggctg acccataggt gttggaggca
     2521 aaggccagtt ttgcccagcc ggaggcacgg gcgtaggcgg gaaaatgggt gtgcgcggtg
     2581 gcgggaacgg cacctgctgc ccctgccgcg ggtcgtagac atccagaggc acgaaggccg
     2641 aacgaatcat ggatttttcg gcggctttct ccaggttgta gcgattctgc tccgccagca
     2701 gggccgcgaa taccccaccc ctgcgtttca actcctggaa gtttccctgt tcggcgattt
     2761 gtccattctt gaggacgatg atctcttcca cgttgccaag cgtgctgagg cggtgagaga
     2821 tcatgataac ggtccgtccg acaaccagtt tttcgagcgc gtgcatcacc tcggcctcgg
     2881 attcgacatc cagggcggcg gtgggttcgt ccaggatcag gatgggcgca tcgcgcagga
     2941 tggcgcgggc aatggccatg cgttggcgct gccctccaga cagattcttg ccctgttcgc
     3001 gtatcgcgcc gtcataaccg ccgaggtctc tgatgatggt ttcgtgaata ttggcctttt
     3061 tcgccgcctc gatcacctcg tagagtggcg cgcccggctt gccaatcgcg atattctcag
     3121 caaccgttcc ctcaaacagc accgagtcct gtaagaccat gctcacattc tggcgcagga
     3181 cggtgagcgg gtacgcgcgg ttatcaacgc cgtcaacctt caccgaaccc tgctgaattt
     3241 cgtagaagcg cgggatgagc ttgaccagcg ttgttttacc gccgcctgac aggccaacga
     3301 gcgcgacctt cttcccggca ggaatatgca gattgatgcc tttcagtaca gcttgctccg
     3361 gggtgtagcc aaagacagcg ttctcaaagc gaatatcgcc cttcaacctg gtcggaccat
     3421 tgtagagaga ctggtagtcc agcacttcgg gggcctggtc caggacttcc tgaatgcgtt
     3481 ccgcgccaga cgccgccgtg ccgtaaaggg ccgtcagctt agagaggtcg cgcataggct
     3541 ggtagagcag attgaggaag atgaggaaca agatcagcgt tccgatatcc acactctcgg
     3601 cttgaacagt gaagaagccc aggttgaagc gggcaccggc ggcgacgtag ccgcctacgc
     3661 caattaccgc cgcagtgccg atagtcacca gaaagccgac gataggggcg aattgcgcct
     3721 gcaagctgcc
//
LOCUS       DASZAK010000642         6287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_122340, whole genome shotgun sequence.
ACCESSION   DASZAK010000642 DASZAK010000000
VERSION     DASZAK010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122340"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..860
                     /locus_tag="VGT44_11245"
     CDS             171..860
                     /locus_tag="VGT44_11245"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV2581414.1"
                     /translation="MDVEKESAYLKQVTFFADLADEDIAQLMSAAKRRTFRAGEVIFH
                     RDDPGQVLYMIKEGKVKICIISPDGQEISLAMFGKGEYFGEFSLLDGQPRSADAIALE
                     KVECYTLQRSDFHNAIMKSPKIAIKVMEALIKRLRSTDEMVEDLIFLDVYGRVAKKLL
                     ELADAHGIQTEGGVRIDVRLTQQELASMVGASRESVNKVMGYFSEKDFISTDKHRITL
                     HRIADLKRRIY"
     gene            976..1992
                     /locus_tag="VGT44_11250"
     CDS             976..1992
                     /locus_tag="VGT44_11250"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: protein motif:HMM:NF008978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HEV2581415.1"
                     /translation="MEKTVVAQQQQSGQQTIDVTPRQPAPRPNAAGRVRALLLALRPK
                     QWTKNLALFVGIVFAQQLFSPALFERTVLAFIAFCLVSSSIYLLNDLLDLENDRQHPT
                     KSKRPLASGALPVSWAITTIGILLALCVLATSLIFFIPIPPKTDIFAPYGGASLAFAA
                     SITAYFVLMVLYSTRLKHVVLLDVFVIAAGFVLRILGGVVVIPVVISPWLYLVTIMLS
                     LFLALNKRRNELILLEQRASSHRQILKEYSLPLLDQMITIVTAATLMAYSLYTFQGPT
                     GNHHLMITIPFVLYGMFRYLYLVHMRGEGGSPEEILLRDRHMLATVTLCAALVIIVLY
                     VLPQ"
     gene            2006..3082
                     /locus_tag="VGT44_11255"
     CDS             2006..3082
                     /locus_tag="VGT44_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV2581416.1"
                     /translation="MQKKFRTGILLSLALAIVVMTAVALYGDLPKMIVVLGQFKWTLL
                     PLILGLSLYNYGWRFIKWQYYLKRLNIHIRWRPSLLIFLSGLSMAITPGKVGELLKSY
                     LLKRSVGEPISRTSPIIVSERLTDGIAMIVLASAGLALYRFGLELMLGLLACVLVGVC
                     IIQNRRLSLALLGFGERLPLISRITAPLRAFYESAYILLRWRPFLLGIGIGVISWTGE
                     CAALYIVYAGLGLGFGLDIFVKSMFILAVSSLIGSASGLPGGLGTADGSMLGLTRLLL
                     TTSATIGSAAVLLIRLCTLWFGLAVGALALLLYRITQHTDILIEQAEKGLSAPTSDDS
                     LDRPLAQEHRPVLHTAATSTSLEK"
     gene            3087..3611
                     /locus_tag="VGT44_11260"
     CDS             3087..3611
                     /locus_tag="VGT44_11260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2581417.1"
                     /translation="MRSLIIIPTYNEYENLGPLLEQIFSFVPGIDLLIVDDNSPDGTG
                     RLADEMADANRLIHVMHRAGKLGLGTAYIAGFKYAVEHGYDVAFEMDADFSHDPRYLP
                     DFLEAIKEADLVIGSRYVPKGGNTELVAGPALHQRRWQYIRPFHAASSHSRLHRRLSL
                     LPPTRVGKHRSGYH"
     gene            complement(3803..4339)
                     /locus_tag="VGT44_11265"
     CDS             complement(3803..4339)
                     /locus_tag="VGT44_11265"
                     /inference="COORDINATES: protein motif:HMM:NF017774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEV2581418.1"
                     /translation="MSVGHEILVDGYNVIKRDASLRALEKKNFTAARDALVTQLAQRY
                     RHTPHRVTVVFDGASSAEQVTHERRVRIIYSRSGEMADQVIARLAAEARTAGLAVEMY
                     SDDREVQTSVTLEGASARSVEQLTSQLNAPPAYHQRLVRHRQAVRARYGLNPSMGKDD
                     DCESPGPPRHSRHSKRKK"
     gene            4601..5605
                     /locus_tag="VGT44_11270"
     CDS             4601..5605
                     /locus_tag="VGT44_11270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2581419.1"
                     /translation="MPNRPAILAEGLKKSYGRTQALVGLDLAAEEGTVLGLLGPNGAG
                     KTTAVRILTTLLQPDSGYAEVTGLDVVKDANALRARIGLAGQYAAVDENLTGFENLKM
                     FGRLYHLSGTVAKRRADELLERFELVDAAGRTVKTYSGGMRRRLDLAASLIMAPPVLF
                     LDEPTTGLDPRGRLSMWDIISDLVADGTTVLLTTQYLEEADQLANQIAVVDHGRVIAR
                     GTSDELKAQMGGERIELSLAQGGDLAAAVQALQPYSAGEIQIDAPRRHLNVPVTNGAQ
                     ILTAVVRDLDAAQIPLDDLALRKPTLDDVFLTLTGRAVTQGVQSESEPETRSMARSSR
                     "
     gene            5602..>6287
                     /locus_tag="VGT44_11275"
     CDS             5602..>6287
                     /locus_tag="VGT44_11275"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2581420.1"
                     /translation="MSEIRITGALKPQTRFPLYWTIADALVLAKRHLVQIPRIPEELV
                     FATIQPVMFVLLFRYVFGGAIAVSGTSYVNYLMAGIFAQTVIFGSTTTGIGLATDLQK
                     GLVDRFRSLPMAKSAVLTGRTISDLVRNVFVVIVMWTVGLLVGFRPQGDVISWFGGAG
                     ILILTSFAFSWISATIGLLVSSVEAAHSAGFIWLFPLTFASSAFVPTSSMPNWLRAFA
                     EHQPVSLIVNA"
BASE COUNT         1299 a         1823 c         1732 g         1433 t
ORIGIN      
        1 tactgttcag ggtagaatgg agagaagacg cctgaaatat ggcaatagta tatcacaacc
       61 ataaaaagcc acttgcgcaa aagcgcgaag tacgatacta ttagacagct agacagccag
      121 ataagacttc atacatgctg agacttgatc aattaaagga agcaatagtt atggatgtag
      181 aaaaggaaag cgcgtatctc aagcaggtta ccttcttcgc tgacctggcc gacgaagata
      241 ttgcgcagct catgtcggcg gcaaaacggc gcacgttccg cgccggtgag gtgattttcc
      301 accgggatga ccctgggcag gtactctata tgattaaaga gggcaaagtc aaaatctgca
      361 tcatcagccc ggatggccag gaaatctcgc tggcgatgtt tggcaaaggc gaatatttcg
      421 gcgaattctc cctgctggat ggccagccgc gctccgctga cgcaattgcc ctggaaaaag
      481 tcgagtgcta caccttgcag cgcagcgatt tccacaatgc tatcatgaaa agccccaaaa
      541 tcgctatcaa ggtcatggaa gcgctcatca agcggctgcg ctcgaccgat gaaatggtcg
      601 aagacctgat cttcctggat gtctatgggc gcgtcgcgaa gaaactgctg gaactggccg
      661 acgcgcacgg catacagacc gaggggggcg tgcgcatcga cgtgcgtctc acgcagcagg
      721 aactggcctc gatggttggc gcctcgcgcg aaagcgtcaa caaagttatg ggctacttca
      781 gcgagaaaga cttcatcagc accgataagc accgcatcac gttgcatcgc atcgccgacc
      841 tgaaacgacg catctattaa cggcgggcag gcgcaataca ggtactagct tctgttactc
      901 cctttcatcg ttgaacgtct ctatgggata ggaagcgtac tgtcttttgt cttgcaaatg
      961 aggaattgtc tttcgttgga aaaaaccgtc gtcgcgcagc aacaacaatc cgggcagcag
     1021 accatcgatg tcactccgcg gcagccagcg ccccgtccga atgcggcggg ccgggtacgc
     1081 gctctgctgc tggccttgcg tcccaaacaa tggacgaaaa atctggcgct gtttgttggc
     1141 atcgtcttcg cccagcaatt gttctcgccc gcgctctttg agcgcaccgt actggccttt
     1201 atcgcctttt gcctggtctc gagcagtatt tatctcttga acgacctgct tgatcttgaa
     1261 aatgatcgcc agcacccgac taaatcgaag cggccgctcg catccggagc gctgcccgtc
     1321 tcctgggcca tcacgactat cggcatcttg ctcgcgttgt gcgttcttgc cacatcgctg
     1381 atctttttca tcccgatccc gccgaaaacc gacattttcg cgccctacgg cggcgccagc
     1441 ctggcctttg ccgcgtcaat cactgcctac ttcgtgctga tggtgctgta tagtacgcgc
     1501 ctgaaacatg tcgttttgct cgatgtcttt gtgattgccg ccggttttgt gctgcgcatc
     1561 ctgggtggcg tggtcgtgat ccccgtcgtc atctcgccct ggctttatct cgtcacgatc
     1621 atgctctcgc ttttcctggc cttaaacaaa cgccgcaacg agttgatcct gctggaacaa
     1681 cgcgccagtt cgcaccggca gatactcaaa gagtacagcc tgcctctgct ggaccagatg
     1741 ataacgatcg tgaccgccgc gacgctaatg gcgtacagcc tctatacctt tcaagggccg
     1801 accggcaatc atcacctcat gattactatt ccattcgtgt tatatggtat gttccgctat
     1861 ctctatcttg tgcatatgcg aggggagggt ggcagccccg aagaaatact gttgcgtgac
     1921 cggcatatgc tggcaacagt cacgttatgc gccgcgctcg ttatcatcgt tctctatgta
     1981 ttaccgcagt aagggaaggt tgacagtgca aaagaaattt cggacgggta tattgctatc
     2041 attggctctt gctatcgtcg tgatgaccgc cgtcgccctg tacggggatc tccccaaaat
     2101 gattgtggtg cttgggcagt tcaagtggac gctgttgccg ctgatcctgg gcctgtcgct
     2161 ctacaattac ggctggcgtt tcatcaagtg gcagtattat ttgaaacgtc tcaatatcca
     2221 cattcgctgg cgaccaagtt tgctgatctt tctgtcgggg ctgtcgatgg cgatcacgcc
     2281 cggcaaggtt ggcgagctgc tcaaatcgta cctgctgaaa cgctcggtgg gcgagccgat
     2341 cagtcgcacg tcgcccatca tcgtctcgga acgcctgacc gatggcatcg ccatgatcgt
     2401 cctggccagc gcggggttgg cgctgtatcg tttcggtcta gagctgatgc tgggcttgct
     2461 ggcgtgcgtg ctggtgggcg tctgcatcat ccagaatcgc cgtctctcac tggcgctgct
     2521 gggcttcggc gagcgcctgc cgctgatctc gcgcatcacg gctccgctgc gcgcgtttta
     2581 cgaaagcgcc tatattctgt tgcgctggcg gccatttctg ctcggcatcg gcatcggcgt
     2641 catctcgtgg acgggcgaat gcgccgcttt gtacattgtc tacgcgggac tggggctggg
     2701 atttggactg gacatcttcg tcaagtccat gttcatcctg gccgtctcct cgttgattgg
     2761 gtcggcttcc ggcctacctg ggggactcgg aacagccgac ggtagtatgt tagggctgac
     2821 tcgtctcctt ctgacgactt cagccacgat cggtagcgcg gcggtgctgc tcattcgcct
     2881 ctgcaccctc tggttcggcc tggcagtggg cgcgctggcc ttactcctgt accgaataac
     2941 ccaacatacc gatatcttaa ttgagcaggc agaaaagggc ctatctgcgc ctacgtcaga
     3001 tgatagcctc gatcgcccac tcgcccagga acatcggcca gtattacata ctgcggctac
     3061 atcaacaagc ttggagaagt aactttatga ggtcgctcat catcattccc acgtacaacg
     3121 aatacgaaaa tttaggccca ttgctcgagc agatattttc atttgttccc ggtatcgatc
     3181 tgctcatcgt tgacgataac tcacctgatg gcacggggag actggctgat gagatggctg
     3241 atgcgaatcg actgattcat gtcatgcacc gggcgggtaa gctcggtctt ggcacagcct
     3301 atattgcagg atttaaatac gcagtcgagc atggctacga tgtcgctttt gaaatggatg
     3361 ccgatttttc gcatgacccc cgctatctcc cagactttct ggaagcaatc aaggaagctg
     3421 acctggtgat tggttcgcgc tatgtcccga agggggggaa caccgaactg gtcgctggtc
     3481 cggcgcttca tcagcggcgg tggcaataca ttcgcccgtt tcatgctgcg tcttcccatt
     3541 cacgattgca ccgccggctt tcgctgttac cgccgacacg tgttggaaag catcgatctg
     3601 gataccattg aatcgcaggg ctatgccttc caggttgaac tggcgtatcg cgtctataag
     3661 cacggtttca agatcgtcga gacacccatc atcttcatgg atcgacgcgt tgggaaatcc
     3721 aagatgtcgc gtaaaatctt catcgaagga tttacctggg tagttcgcgc gcgcttcagc
     3781 gggcagaaac aaaagatatc tgctacttct tccgtttcga gtggcgcgaa tgtcgtggag
     3841 gacccggcga ctcacaatca tcatccttcc ccatcgacgg attcagccca tagcgagcgc
     3901 gaactgcctg gcgatgacgc accagccgtt ggtgataagc cggaggagca ttcagctgtg
     3961 aggtaagctg ctcgacggaa cgcgcgcttg ccccttctag agtgaccgag gtctggactt
     4021 cgcggtcatc gctatacatt tccaccgcga gtccagcggt gcgcgcctct gccgcgaggc
     4081 gcgcgatcac ctggtcggcc atttcgccgc tgcgggagta gatgatccgc acccgtcgct
     4141 catgcgtcac ctgctctgct gaagatgcgc catcgaagac gaccgtcaca cggtgtggcg
     4201 tatggcgata acgctgcgca agttgcgtca ccagtgcgtc gcgcgccgcc gtaaaattct
     4261 tcttctccaa cgcacgcagc gacgcatccc gtttgatcac attatagcca tcaaccagaa
     4321 tttcatgacc cacgctcact ttgtacctcc cttaaatgca gctcgatatt cattacacga
     4381 tagatgatcg cgcctggaat gtcaaccatg cgcgactcag ctttgcaaaa tttcaaacct
     4441 gtgctattat attttcatgg atataaaagt tatgtttgat gcccgtaaat cattagaagc
     4501 gcaacccagc catatcaggc tcgtataact gatagctggg aagctattgt gactatgcca
     4561 caacatgaat ggcaaggcat tgacatggga ggaagaaatc atgccgaata gaccggctat
     4621 tttagccgag ggactcaaaa aaagctatgg gagaacccag gccctggtgg gactcgacct
     4681 ggctgccgag gaaggaaccg tgctgggctt gctcgggcca aatggcgcgg gcaagacgac
     4741 tgccgtgcgc atcctgacga cgctgctgca acccgatagc ggatacgcag aagtcacggg
     4801 actcgacgtg gtgaaagacg ccaacgccct gcgcgcccgc atcggcctgg cgggtcaata
     4861 tgcagccgtc gatgaaaacc tgaccgggtt tgaaaatctg aaaatgttcg ggcgactcta
     4921 ccatttatcc ggcacagtcg ctaagcggcg ggcggacgag ttgctcgaac gttttgagct
     4981 ggtcgatgcc gccgggcgca ccgtcaagac ctactccggt gggatgcgca ggcgactcga
     5041 tctcgcggcc agccttatca tggcgccgcc ggtgcttttc ctggacgagc caacgaccgg
     5101 ccttgacccg cgcggtcgcc tgtccatgtg ggacatcatc agcgatctcg tcgccgacgg
     5161 caccactgta ctgctcacca cgcagtacct tgaggaggcc gatcagctcg ctaaccagat
     5221 cgccgtcgtc gatcacgggc gtgtgatcgc cagaggcacg tccgacgaac tgaaggctca
     5281 gatgggcggt gaacgcatcg aactctcgct ggcccaggga ggcgacctcg ctgcggctgt
     5341 acaggcgctg caaccatata gcgcgggcga gattcagatt gacgcgccac gcaggcatct
     5401 caatgtgccg gtcacgaatg gcgcgcagat cctcaccgcc gtcgtgcgcg acctcgacgc
     5461 cgcgcagatt cccctggacg acctggcctt gcgcaagcca accctggacg atgttttcct
     5521 cacgctgacg ggccgtgccg tgacgcaggg ggtgcaatct gaatccgagc ctgagacgcg
     5581 ctcgatggca aggagttctc gatgagcgaa attcgcataa ccggagcatt gaagccgcaa
     5641 acgcgcttcc cattgtattg gacgattgct gacgcgctcg tcctggcgaa acgccacctg
     5701 gtgcaaatcc cacgcatccc ggaagaactg gtcttcgcca cgatccagcc tgtgatgttc
     5761 gtcctgctct tccgctacgt gttcggcggg gccatcgcgg taagcggcac cagctacgtc
     5821 aactacctga tggccggcat tttcgcgcag accgtcatct tcggctcgac gacgaccggc
     5881 atcggactcg ccaccgacct gcaaaagggc ctggtcgacc gtttccgctc gctgccgatg
     5941 gcgaaatcag ccgttctgac cgggcgcacc atttcagacc tggtgcgcaa cgtcttcgtc
     6001 gtcatcgtga tgtggacggt cggcctgctg gtcggctttc gaccgcaggg cgatgtcatc
     6061 tcctggttcg gcggcgcggg catcctcatt cttaccagtt tcgctttttc gtggatttcc
     6121 gccacgattg gcctgctggt cagcagcgtg gaggcggcgc attcggctgg cttcatctgg
     6181 ctcttcccgc tcaccttcgc cagcagcgcc ttcgtgccaa ccagcagtat gcctaactgg
     6241 ctgcgcgctt tcgcggaaca ccagcccgtc tcactgatcg tcaacgc
//
LOCUS       DASZAK010000643         8318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_656375, whole genome shotgun sequence.
ACCESSION   DASZAK010000643 DASZAK010000000
VERSION     DASZAK010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8318
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656375"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..813
                     /locus_tag="VGT44_11280"
     CDS             <1..813
                     /locus_tag="VGT44_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2581421.1"
                     /translation="DPQKIRELLTFVLVGGGPTGVETAGAIAELAHRALAYDFRHIDT
                     KLTRIILIEAAPRILMAFPESLASKTRRKLNSMGVEVIEGRAVTQVDECGVVVDGERI
                     NALTILWNAGVTASPAGQWLGAEVDRTGRVKVEGDLSVPNHPNVFVIGDTACVMQDGK
                     PLPGVAAVAMQAGHYIASVIARCATGKRQHAPFRYRNRGSLATAGRSYAILEMGKLRM
                     TGLAAWVAWLVVHIYYLIGFRNRLVKMVEWAWTYMTYSRGARLITFEKDREQ"
     gene            complement(828..2483)
                     /gene="cydC"
                     /locus_tag="VGT44_11285"
     CDS             complement(828..2483)
                     /gene="cydC"
                     /locus_tag="VGT44_11285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HEV2581422.1"
                     /translation="MREMLPYVTLIRGKLLRKFLLGLSLACLAGLAAVGLVGLSGWFI
                     ATSALVGLLGITTFSFLFPSGGVRAFALLRSAARYGERVVNHKATFVFLARLRVLAFA
                     RASRLPLRNITRYRSGDLLTRLMADIDTLDQLLVRILIPTLSTAMVVLVSLIFLAGYS
                     AILAACTGIIVIAAGVVLPLLMARLGRRSGVQFVEARAEARTRCVEALQGRREIASYQ
                     AEPIVRQQVGQALTRADEVQSGQLHAAAISSALMSALASTALLATLLISVSLVTKATL
                     SGPIIVMLYLVVMAVFECIEGLPQAYQFLGQTRHAARRLNALLPAMEPAPAPSPAQPA
                     GVWQQAIRVEQVSFHYDEQRAPVFEDFTCLLPLRSLVAVTGPSGAGKSTLLKLLAREL
                     EPGRGTIALGETPLACIQAQELYRRQALISQDSHIFNASLRENLLMAKPDATEQEMRD
                     VLEAVCLTSLLDKLADGLDTSLGEQGEMLSGGERRRLSLAQALLKLPSLLLLDEPTTG
                     VDRETAQRILATLRARLPQSTIVVATHEAWLASLADHHVVLPA"
     gene            complement(2476..4320)
                     /gene="cydD"
                     /locus_tag="VGT44_11290"
     CDS             complement(2476..4320)
                     /gene="cydD"
                     /locus_tag="VGT44_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HEV2581423.1"
                     /translation="MSAASSDQAASKRFLRAYLAHGRRFVTATSLATSGTTLAIILQA
                     FAFARSIQLVVIEHAALASVVPWLFVFGAGSILRCACAYFAQRWPAEGALRIQQRLRR
                     AMLETLFVSQGPVMASADAQTAETAHTLLEQVDKLEPYYTRYIPRLVGTALCPLLFLV
                     VIFPTDWVSGVILLLTTPVIPFYMALIGKGAESVSRKQMAITRGLSATLLDYLQGMQT
                     IKALGATARAEQTIADASHELSERTMAVQRIALLSSAILEFFSTFAIAILALYVGLSL
                     LHYIQLGNSPTGMSLQTGLFVLLLAPSYFQPLRAFAAAYHDRVDALTAAAHIDALLQA
                     APPPETAQSQQRAQEVMQQIELRHVTLRYPGRTSPALSDISLRIDQGEKVALVGPSGA
                     GKSSLLSILAGFTGISEGEIYMNGRPPAETAPVHVAWIGQRPYLFPGTLAENIALGRP
                     QASRQEIEAAASEARVMDFAAALPAGLNTLIDERGLGISGGEAQRVALARAFLANAPL
                     LVLDEPTAHLDADTEAALVETIAALAQGKTLVLATHSPALLVLCERVLSLDEGRLLPQ
                     PAPTLPLALPAMSPEEDPMRRDLLFRSPTRLVPLPLRGQETGEEVHHA"
     gene            complement(4317..4538)
                     /locus_tag="VGT44_11295"
     CDS             complement(4317..4538)
                     /locus_tag="VGT44_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581424.1"
                     /translation="MNQLNGSRTRPRSALYGVITVAWMVLGMALSMMCIKIFGNNTAA
                     IGVPALVVASALIWYFTGRRDRHGSEENL"
     gene            complement(4535..5650)
                     /locus_tag="VGT44_11300"
     CDS             complement(4535..5650)
                     /locus_tag="VGT44_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEV2581425.1"
                     /translation="MATPPTPFPIGAFIWLLVAVMSLTMYMALDGYDLGVGILMLTES
                     DTNRHKEMMEIIATAWDGNESWFVLLGISLFAGFPLAYSIILPAFYLPFILMLLSLVF
                     RGMSLEFQSHSPNYERKYSLAFSLGSLVATFCQGVMVGAFVTGIPVQHGHFVGTPFAF
                     LNGYSLLAGLFFVCSFCLTGASWLNDKMEGEPAANAIRKGRILTPVVGVLLVALAILA
                     PFVSPVVGNALSTIQLLTTIGTTLLALIFLVLTWYTLKHLGNVLPFLFAVFALALPLL
                     NLLILSYPYVVPASVTFWQAAAPQSSVNFLLVGVGSCIPIVLAYNTYAYYVFRGKFTP
                     YFIHRQSAGIAASALAAVSHADRNDVTNVEPERSEGI"
     gene            complement(5681..7051)
                     /locus_tag="VGT44_11305"
     CDS             complement(5681..7051)
                     /locus_tag="VGT44_11305"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEV2581426.1"
                     /translation="MNTQLLDLSRLQFALTVGFHMIFPSITVGLSLFLVLLYGSYLKT
                     NKPIYLTMYRFWRNIFAVGFGLGVVAGIVITFEFGLNWAGYANAVGPIVGVIISMEVI
                     TAFFLEAGFLGIMLYGEGRVSQRVMFIANCMVALGTLLSTTWILSANSWMQTPAGYTE
                     VNGQFRPTNWAAAIFNPSFFYRYPHMLLAVLLSSAFLVTGVAAWYLLKRQHLEFARRT
                     FSIGLGVIALLIPLQLFMGDTVAFAMISYEPPKMLAAEGNWTSTNTGDYLFIITDQQD
                     QRNIVQIGIPWIGSIIWYHDFSLTRAVPGLDLVPPALQPPMAFVFYGFHLMVYLALLM
                     FFVGFFSLLLRLRGRLYTSRWFHWLALAMTPAGVIAIIGGWVTAETGRQPWVVYGKLL
                     TSSAVSPLSPGLVIASLIGFIATYVTLLTIYIVYMVRAVRQGPEDVPALAPLPGSPGE
                     MQVPVS"
     gene            complement(7208..7894)
                     /locus_tag="VGT44_11310"
     CDS             complement(7208..7894)
                     /locus_tag="VGT44_11310"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEV2581427.1"
                     /translation="MDTIKDEKETGRLEGFSDGVIAVAITLLVLALQPPSKTSNVDLL
                     SYLWQQWPFFLAFVTSFATVGVMWINHHRMFSYIKRADSTLMSLNLLLLLFIVFIPFP
                     TALVAQYLTSPKNERVAALLFNGTYFLLAICFNLLMRYATHENRLLGKDVNQREIEAM
                     LKQFRFGPLAYLVTFALTWVSVPVSLALNLLLALFWALPVLKRPSFIPESLPFKARTK
                     RTGTTGEVEE"
     gene            complement(7999..>8318)
                     /locus_tag="VGT44_11315"
     CDS             complement(7999..>8318)
                     /locus_tag="VGT44_11315"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV2581428.1"
                     /translation="LFLTGDQPLITAQIIDSVIEAFEAGRREGKRIVAPLYGGQRGNP
                     VLFDAALFDELMEVTGDEGGRGLLKRHPDEVATIETGNPAASSDVDTWEAYQQVLRKW
                     DEQ"
BASE COUNT         1924 a         2337 c         2577 g         1480 t
ORIGIN      
        1 gacccgcaga agattcgaga actgctgacg tttgtgctgg ttggcggagg gccgacgggc
       61 gtcgagacag cgggggcgat agcggaactg gcgcatagag cgctggccta cgattttcga
      121 catatcgata cgaagttaac acgcatcatc ctgatcgaag cggctcccag gatactgatg
      181 gcctttccag aatcgctggc gagcaaaacg cgacgaaaat tgaacagtat gggagtggaa
      241 gtaatcgaag gcagggcagt gacacaggta gatgagtgtg gagtagtcgt tgatggcgag
      301 cgcatcaatg cgctcaccat cctctggaac gccggcgtga cggcatcccc ggcgggtcaa
      361 tggctggggg ccgaagtcga tcggactggc cgcgtgaagg tggagggcga cctgtcggtt
      421 cccaaccacc ccaatgtgtt tgtgattggc gacacggcct gcgtgatgca ggatggcaag
      481 ccgcttccag gagtcgcggc agttgcgatg caggcgggcc actacatcgc ttcagtgatt
      541 gcccggtgcg ccacggggaa gcgccagcac gcgccatttc gctaccgcaa cagaggcagt
      601 cttgccaccg cggggcgttc ctacgccatc ctcgagatgg ggaaactgcg catgacaggt
      661 ctggctgcct gggtggcgtg gctggttgtg catatctact acctgatcgg gttccgcaat
      721 cgcctggtca agatggttga gtgggcatgg acgtatatga cctattcacg tggggcgcgg
      781 ctcatcacgt tcgagaaaga ccgcgaacag taggcaactt ctcctcatca tgccggtaag
      841 acaacatggt ggtctgccag cgatgcaagc cacgcttcgt gtgtggccac gacaatcgta
      901 ctctggggga gacgcgcgcg aagcgtcgcc agtatgcgct gagccgtctc cctgtccact
      961 cccgtcgtcg gttcgtcgag gagcaagagg gaggggagct tgagcaatgc ctgggccagg
     1021 ctcaggcggc gacgttcccc gcctgaaagc atctctccct gttctcccag actcgtatca
     1081 agtccatccg cgagtttgtc aagcaggctg gtcaaacaga ccgcctccag aacatctcgc
     1141 atctcctgct ctgtcgcatc aggctttgcc atcagcaaat tttcccgcag gctcgcattg
     1201 aaaatgtgac tgtcctgcga gatcagcgcc tgacgccggt acaattcctg cgcctgaata
     1261 catgccaggg gcgtctcgcc aagcgcaatc gtgccacgac ccggctcaag ttcgcgcgcc
     1321 aggagcttca ggagggtcga tttgcctgcg ccgctcggcc ccgtgacggc caccagcgac
     1381 ctcaacggga gaaggcaggt gaagtcctcg aacacagggg ctcgctgctc gtcgtagtga
     1441 aaagacacct gctctacacg aatggcctgc tgccatacac ccgctggctg agctggagac
     1501 ggtgcgggcg caggttccat cgctggcagc agggcattga gccgccgggc ggcatgcctt
     1561 gtctgaccca ggaattgata ggcttgaggt agaccctcga tacactcaaa gacagccatt
     1621 accaccaggt aaagcatgac gatgattgga ccgctcagcg tggcttttgt tacgagactc
     1681 acgctgatca agagggtggc aagcagggcg gtagaagcaa gagcggacat caacgccgag
     1741 ctgatcgcag cagcgtggag ctgtccgctc tgcacttcgt cggcgcgtgt cagcgcctgg
     1801 ccgacctgtt gcctgacgat aggctccgcc tggtaggacg cgatttctcg tcggccctgt
     1861 aacgcctcaa cacagcgggt gcgcgcctct gctcgcgcct ccacaaactg gacgccggac
     1921 ctgcgtccga gacgcgccat caggagaggg agcactacgc cggcggcgat cacgataatt
     1981 ccggtacacg ccgccaggat agcggagtag ccagcgagaa agatgagcga aacgagaacc
     2041 accatcgcgg tcgagagcgt tggaatgagg atgcgcacga gcaactggtc gagcgtatcg
     2101 atatcggcca tgaggcgagt gagcaggtcg ccgctgcgat agcgcgtgat gttccgcaag
     2161 ggcaagcgtg atgccctggc aaaggccaat acgcgcagtc gcgctagaaa gacgaaggtc
     2221 gccttgtggt tcaccacacg ctccccataa cgggcagcgg aacgcagcaa ggcaaaggcg
     2281 cgcaccccgc cgctcggaaa caggaacgag aaggtggtga tccccagcag gccaacgagc
     2341 gccgaggtgg cgataaacca accggagagc cctaccaacc cgacggcggc cagacccgca
     2401 aggcaagcaa gcgagagccc aagcagaaac ttgcgcagga gttttcctcg gatgagcgta
     2461 acgtaaggta acatttcacg catggtgcac ctcctctccg gtctcctgac cgcgcagcgg
     2521 tagaggtact agcctcgttg gagaccggaa taagagatcc cgccgcatcg ggtcctcctc
     2581 tggcgacatg gccggcagcg cgagtggtag cgtaggagca ggctggggca gcagtctccc
     2641 ttcatccagg gagaggacgc gctcgcagag cacgagcaga gccgggctgt gcgtcgccag
     2701 gaccagagtt ttgccttgtg cgagcgccgc aattgtctcg acaagcgccg cctcggtgtc
     2761 cgcgtcaaga tgggctgtag gttcatcaag cacgagcaag ggggcattgg cgagaaaggc
     2821 gcgcgccagc gcgacgcgct gcgcctcgcc gccagatatc cccagaccgc gttcgtcaat
     2881 cagcgtattc aggcccgcag gcagcgcggc ggcaaaatcc atgacacgcg cctccgacgc
     2941 tgcggcttcg atctcctggc gcgaagcctg cggacgcccc agggcgatgt tttccgcgag
     3001 cgtccccgga aagagatagg gacgctgacc aatccaggcg acatgcaccg gagcggtttc
     3061 tgcaggcggg cggccattca tgtaaatctc tccttcggat atgcccgtga aaccggcgag
     3121 aatcgagagc agagaagatt ttcctgcccc ggaagggccg acgagcgcga ctttttctcc
     3181 ctggtcgatg cgcagcgaga tgtcggagag cgcgggactt gtgcggccag gatagcgcag
     3241 cgtgacgtga cgcaactcga tctgctgcat cacctcctgc gcccgttgct ggctttgcgc
     3301 tgtttccggc ggcggcgctg cttgcagtaa agcgtcgata tgagccgccg ccgtgagagc
     3361 atcgacgcga tcatggtagg cggcagcaaa ggcgcgcagg ggttgaaagt aggagggagc
     3421 caggagcaag acgaacaggc ctgtttgcag tgacatgccg gtcgggctgt tgccaagctg
     3481 aatgtagtgg agcaggctaa gaccgacgta gagggcgagg atcgcgatgg cgaacgtact
     3541 gaaaaattcc aggatggcag aggagagtaa ggcgatacgc tgtacggcca tggtgcgctc
     3601 gctcagttca tgggaggcgt cggcaatcgt ctgctcagcg cgcgcggtcg cccccagcgc
     3661 cttgatcgtc tgcatccctt gcagataatc cagcagcgtt gcagagaggc cgcgcgtgat
     3721 ggccatctgc ttgcggctga ccgactcagc ccccttgccg atcaacgcca tataaaatgg
     3781 aataaccggc gtcgtcagca acaggatgac gcctgacacc caatcggtcg ggaaaatcac
     3841 gacgaggaac agcaaggggc agagcgctgt tccaaccagt cgagggatat agcgtgtgta
     3901 atacggttcg agcttgtcca cctgttccag cagcgtatgc gcggtttccg ccgtctgtgc
     3961 gtcagcagaa gccatgacgg gtccctggga gacgaagagc gtctcaagca tggcgcggcg
     4021 cagccgttgc tggatgcgca gcgcgccttc ggcaggccag cgttgcgcga agtaggcgca
     4081 ggcacagcgc aatatactcc cagcgccgaa gacaaagagc cagggaacaa cagacgcaag
     4141 cgcagcgtgt tcaatcacca ccagctggat cgaacgggca aaggcaaagg cctggagaat
     4201 aatcgccagg gtggtgccgc tcgtcgccag cgaggttgcc gtgacgaacc gccgaccatg
     4261 cgcgaggtag gctctgagaa agcgcttgct ggcagcctga tcgcttgaag cggcgctcat
     4321 aggttctcct ccgaaccgtg acgatcccgc cgcccggtaa agtaccagat gagcgccgag
     4381 gcgacgacca gcgcgggaac cccaattgcg gcggtgttat tgccgaagat cttgatacac
     4441 atcatggaaa gagccattcc tagcaccatc caggcgacgg tgatgacccc gtataaggcg
     4501 ctgcgtgggc gggtgcgact gccattcagc tggttcatat gccctcactc ctttccggtt
     4561 cgacgttggt gacatcattc cgatcggcat gactgacagc agccagcgcg ctggccgcga
     4621 tgccagcgct ctgccggtgg atgaagtagg gggtgaactt gccgcgaaac acgtagtagg
     4681 catacgtgtt ataggccagc acaatcggga tgcagctgcc aactccgacg agcagaaagt
     4741 tcacggaact ttgcggcgcg gcggcctgcc agaacgtgac ggacgcggga acgacatagg
     4801 gatagctgag gatcagcagg ttgagcagcg gcagtgcgag cgcgaaaacg gcgaagagaa
     4861 aggggaggac attccccaga tgcttgaggg tgtaccaggt gagaacgaga aaaataagcg
     4921 ctaagagcgt cgttccgatg gtcgtgagca actgtatcgt cgaaagggcg tttccgacca
     4981 ccgggctgac gaaaggcgcc aggatcgcca gagccacgag cagaacgccc acgaccggcg
     5041 tgaggattct ccctttgcga atggcattgg ccgccggttc cccttccatc ttgtcgttaa
     5101 gccagcttgc gcctgtcaga cagaagctac agacgaagaa gagtcccgcg agaaggctat
     5161 agccgttcag gaaagcgaac ggcgttccga caaaatggcc atgctgcaca gggataccgg
     5221 tgacgaacgc tccgaccatg accccctggc agaaggttgc tacaagcgac cccagggaaa
     5281 aggccaggct gtattttcgc tcgtagttcg ggctgtgcga ctggaactcg agggacatgc
     5341 cccggaagac gagcgacagc agcatgagga taaaggggag atagaatgcc gggagaatga
     5401 tgctataggc cagcggaaat ccagcgaaaa gagagattcc gagcaggacg aaccaggact
     5461 cgttaccgtc ccaggccgtt gcgataatct ccatcatctc cttgtgccgg ttcgtatcgc
     5521 tctcggtgag catgagaatg cccacgccca ggtcgtagcc atccagggcc atatacattg
     5581 tcaggctcat cacagccacc aggagccaga taaaagctcc aattggaaat ggggttggag
     5641 gtgtagccat tgaagttctc ctctttatat gacctattag ctagctgacc gggacttgca
     5701 tttcgcctgg actgcctgga agaggcgcaa gagcgggtac atcctcaggc ccctggcgga
     5761 ccgcgcgaac catgtaaaca atatagatag tgaggagcgt cacataggtg gcgatgaagc
     5821 cgatcaagga ggcgatcacc aggcccggag agaggggcga aaccgctgaa ctggtcaaga
     5881 gcttgccgta gacgacccag ggttgccgac ccgtctccgc cgttacccag ccgccgatga
     5941 tggcgatcac cccggcaggc gtcatcgcca gggccagcca gtgaaaccac ctggaggtat
     6001 agagccgccc tctcaggcgc agcagcagac tgaagaaacc cacaaagaac ataagaaggg
     6061 ccaggtagac catcaggtga aagccgtaga acacaaacgc catcggcggc tgcaaggcgg
     6121 gcggtaccag gtcaagacct ggaacggccc tggtcaagct aaaatcgtgg taccagatga
     6181 tactgccaat ccatggaatg ccaatttgca caatgttgcg ctggtcctgc tgatccgtaa
     6241 taataaacag gtaatcgccg gtgttggtcg aggtccagtt gccctcggcg gcaagcatct
     6301 tcggcggctc gtaggagatc atggcgaagg caacggtgtc tcccataaat aactggagcg
     6361 ggatgagaag agcgatgacg cccaggccaa tcgagaacgt gcggcgcgca aactccaggt
     6421 gctgccgttt gagcagatac caggcggcga ctccggtgac cagaaaggct gaggagagca
     6481 ggacggccag cagcatatgt ggatagcggt agaaaaacga tggattgaaa atagcggcag
     6541 cccagttggt gggacgaaac tgcccattga cctcggtata ccccgcgggc gtttgcatcc
     6601 aggagttcgc tgagagaatc caggttgtag agagcagagt ccccagagcg accatacaat
     6661 tcgcaatgaa catgacacgc tggctgacac gtccctcgcc gtagagcatg atccccagaa
     6721 aaccggcttc gagaaagaag gccgtaatga cttccatact gatgatgacg cctacgatgg
     6781 gaccgacggc gttggcataa cccgcccagt tgaggccgaa ctcgaacgta atcacaatgc
     6841 ccgccacgac gcccagcccg aagcccacag cgaaaatgtt ccgccagaag cggtacatgg
     6901 tgaggtagat gggcttgttg gtcttgagat aggacccgta caggaggacg aggaagagac
     6961 tcagcccgac ggtgatagag ggaaagatca tatggaaacc taccgtcagt gcgaattgaa
     7021 gtcgcgacaa atcgagcaat tgcgtgttca tcgagaaatc ctttccgttt tgatcgacag
     7081 atcgttactt gcccggtctt ctcgctcatc tcgaaaccta ccgcgtatta gacggaaggg
     7141 agttaacaat actagtatac tctgtgcagc gtttcctgag cgtttcctat gctggccgtc
     7201 gacctggcta ctcctcaacc tcgccggttg tgccggtgcg cttggttctg gctttgaagg
     7261 ggagcgattc gggtatgaac gaggggcgtt taagcaccgg cagcgcccag aagagggcca
     7321 gcagcaagtt gagtgcaagg ctgaccggca cgctgaccca ggtcagcgcg aaggtaacga
     7381 ggtaggccag cggcccaaaa cggaattgct tgagcatagc ttcgatttcg cgctggttca
     7441 catctttccc cagcagccgg ttctcatgcg ttgcatagcg cataagcagg ttgaagcaga
     7501 ttgccaacaa gaaataggtg ccgttgaaaa ggagtgcagc gacgcgctcg tttttgggcg
     7561 aagtgagata ctgggccacc agtgctgtgg ggaagggaat gaaaacgatg aagagcaaga
     7621 gcagcaggtt aagcgacatt aatgtagagt ctgctcgctt gatgtacgaa aacatacgat
     7681 ggtgatttat ccacatcacg ccaactgttg cgaagctcgt tacgaaagcc aggaagaacg
     7741 gccactgctg ccataggtaa ctgagtaaat cgacatttga tgtttttgag ggtggctgca
     7801 aggcaagcac cagcagcgtg atggcaacgg caatcactcc atcgctgaag ccttcgagcc
     7861 gcccggtctc tttttcatct ttgatagtat ccatagcagg tagtatacat gctcccacgc
     7921 ctgctgacaa cgacgaaatt gccaggagat aacctggaaa gatcatcagg aaaacactat
     7981 gcgcacgaca tcccggcgct actgctcatc ccatttccgc aaaacctgct ggtacgcctc
     8041 ccacgtatca acatcggagg atgccgccgg gttcccggtc tctatagttg ctacctcgtc
     8101 tgggtgcctc ttgagcaggc cgcgcccgcc ctcgtcaccc gtcacctcca tcaattcatc
     8161 gaagagagcg gcatcaaaca acaccgggtt gccgcgctgc ccgccgtaga gcggcgcgac
     8221 gatacgcttt ccctcgcgcc tgcctgcctc gaatgcctcg atgacgctgt cgatgatctg
     8281 cgctgtaatc agcggctgat cgcccgtgag aaagagcg
//
LOCUS       DASZAK010000644         3287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_299813, whole genome shotgun sequence.
ACCESSION   DASZAK010000644 DASZAK010000000
VERSION     DASZAK010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299813"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..524
                     /locus_tag="VGT44_11320"
     CDS             297..524
                     /locus_tag="VGT44_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581429.1"
                     /translation="MSSSDHHTTTTWPGHFSAVLEPRFFGGQQPNRQNRHPGADFAAD
                     WGGFCERPAVHGTMMHNLRRSGQEESEATSP"
     gene            complement(586..1104)
                     /locus_tag="VGT44_11325"
     CDS             complement(586..1104)
                     /locus_tag="VGT44_11325"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2581430.1"
                     /translation="MEENVYRTELTPVSFLQRSAFVFPDKTAVIHGDRQYTYREFAER
                     VNHLASSLRAFGLQKHDRVAFLCPNTPALLEAHFAVPAAGGILVAVNSRLNAQEIDYI
                     LQHAGAKFLFAGRLEGGGGVLQGPAIVRRRVPAALGFLWDPCENRSQESLAIAMADAL
                     TLDRCAPRRSVR"
     gene            complement(1167..1520)
                     /locus_tag="VGT44_11330"
     CDS             complement(1167..1520)
                     /locus_tag="VGT44_11330"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581431.1"
                     /translation="MKGDMISVVGYEVSPMEVEEGLLLHPAIAEAAVIGVPDAYLGQA
                     IKAFVSLKPGASATPKEIIAFCKERMPRHQYPRQVEIVDDIPKSETGEILRGELRAQE
                     SAKLRGQRQPHPREP"
     gene            1628..1861
                     /locus_tag="VGT44_11335"
     CDS             1628..1861
                     /locus_tag="VGT44_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2581432.1"
                     /translation="MLVSRAKLGAAVAEARAKAGLTQAELAQRAGLDEATLAALEQGK
                     YKPESHELSRLAEALGVDELELLRRPAPGYLII"
     gene            complement(1854..3146)
                     /locus_tag="VGT44_11340"
     CDS             complement(1854..3146)
                     /locus_tag="VGT44_11340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2581433.1"
                     /translation="MQQQFVIVGANLAGGTAAITLREEGFDGEIVLIGAEPHLPYERP
                     VLSKEYLQGKAPFEEALLKPLTFYAEHTIQTRLGVRATRVDPREQVVELEDGDRVHYD
                     KVLVATGVRNRRFRLPGMELERIYDLRTVADADLIRAESVKGRRAVLVGMGFIGCEVA
                     ASLRHYDVEVTVVESFKTPLCRVLGEQVGRAFEAFHREHGVQMFFEETVTAFEGRRHV
                     QRVVTSGGRRLDCDFVVVGIGVEPVTDILAGTDVKIENGIVVDEYCQTNVEGIYAAGD
                     VANHAHPVFGRLVRVEHWQNAIHQGRAAARSMLGKREPYSDVHWFWSDQYDYRVEYAG
                     FHSGWDELVVRGSLEERNAVVFYVQEQRIAAAVAINRGKDVQRVMPLIKARIEVDPDK
                     LRDENIDLRSLMPGAKPVRTHQHRVAPHPVARPGEEAR"
BASE COUNT          668 a         1050 c          910 g          659 t
ORIGIN      
        1 tcagttctct ttccagttgt gctccattgc gtgtaaattt tggaattaaa tgggcggaat
       61 ctagctaatt atgctggcgg taaaacgagc aaaaacagag atgacatgta tggcttcacc
      121 ctttcgctcg tcccatttcg gctcggtagg ctgtttcgca cattgccggc tcaacagcgg
      181 aattcgatag aatctgccca tttaattcca aaatttacaa ccagagcacc ccgagagaaa
      241 gagccaacgc cgaaatgtga gcagcaaccc tcaccaacgc gtctgagacg cgatttttga
      301 gctctagcga ccatcacacc accaccacct ggccaggcca cttctcggcc gtcctggagc
      361 ctcgtttttt cggcggccag caaccaaatc gacagaatcg tcatcccggg gctgatttcg
      421 ctgccgattg gggtggtttt tgtgaacgcc cggcggtgca tggcacgatg atgcacaact
      481 tgcgccgatc tggtcaggag gaatccgagg ctaccagccc ttgacgaatt cacgcaggtg
      541 atggtaccat gatgccacca accgcctggt gacggaacca gggtctcagc gaacggatcg
      601 tcgcggcgcg cagcgatcca gtgtcagcgc atccgccatg gcgattgcca aagattcctg
      661 tgatcgattt tcgcaaggat cccagaggaa cccgagagcc gcaggcacac gacgacgaac
      721 aattgccggg ccctggagta cccctccccc accttctaag cgtccggcga agaggaactt
      781 cgcgcccgca tgctgcaaga tgtagtcaat ctcctgcgca ttcagccgac tgttcacggc
      841 aaccaggatg cccccggcgg cgggtacagc aaagtgagcc tcgagcaagg caggcgtatt
      901 cgggcacaag aaggcaactc gatcatgctt ctgtagaccg aaggcacgca gactggatgc
      961 caaatggttc acccgctcgg caaactcgcg gtaagtatac tgccgatcac cgtggatcac
     1021 tgccgtttta tctggaaata cgaaagcact acgctggagg aaactcaccg gcgtcagttc
     1081 ggttcgatag acgttctctt ccatttgagg ctccttgtcc aaacaactgt caacccgccc
     1141 tccaagcaac accttcctgt accgggtcat ggctcgcgtg ggtgcggttg ccgctggccg
     1201 cgcagcttcg ccgattcctg cgcacgcagt tcacctcgca agatctcacc ggtctcactc
     1261 ttcggaatgt cgtcgacgat ctccacttgc cgtggatact ggtgcctggg catccgctcc
     1321 ttgcagaagg cgatgatctc cttcggtgtg gcagaagctc cgggcttgag cgagacaaag
     1381 gccttaatcg cctggccgag gtacgcatcg ggcacaccga tcacggccgc ttccgcaata
     1441 gctggatgga gcagcaggcc ttcctcgacc tccatcggcg agacctcgta gccaaccacg
     1501 ctgatcatat cacctttcat cgctttcctc ctggactcta gaccgttggt aatctgtagc
     1561 aaactgtggt aatcttgctc tatggttatc tgttgtagac cctcatcaag gagagaaggg
     1621 caatacgatg ctcgtatcac gcgccaaact cggagcagcc gtggcggagg ctcgggcgaa
     1681 ggcaggactc acgcaagcag aactcgcgca gcgtgctggc ctggatgagg ccacgctcgc
     1741 ggcactagag caggggaaat ataagcccga atcgcacgaa ctgagtcggc tggccgaggc
     1801 gctcggcgtg gacgagttag agctcctgcg cagaccggct cccggatacc tcatcatcta
     1861 gcctcctcgc ccggacgcgc caccgggtgc ggcgcgacgc gatgctggtg cgtccgcacc
     1921 ggcttggcac cgggcatgag cgagcgcagg tcgatgttct catcccgcag cttgtcggga
     1981 tcaacctcaa tgcgcgcttt gatcagaggc atcacgcgct gcacatcctt gccccgattg
     2041 atggcgacgg cagcggcaat gcgctgctcc tgcacataga acacaacggc attgcgctcc
     2101 tccaggctgc cacgcacgac cagttcgtcc cagccgctgt ggaagcccgc gtactccact
     2161 ctgtagtcgt actggtcaga ccagaaccag tgcacgtcgc tgtagggctc gcgcttgccg
     2221 agcatgctgc gagcagctgc ccgtccctgg tggatcgcgt tctgccagtg ttcgacacgg
     2281 accaggcggc caaagacggg gtgcgcgtga ttggccacat ccccggcggc gtagatgccc
     2341 tccacgttgg tctggcaata ctcgtcgacc acgatgccgt tctcgatctt cacatcagta
     2401 cctgccagga tgtcggtaac gggctcgacg ccgatgccga ccacgacgaa atcacagtcg
     2461 agccgtcgtc cgccgctcgt cactacacgt tgcacgtgac gcctgccttc aaaggccgtc
     2521 accgtctcct caaagaacat ctgcaccccg tgttcgcgat ggaaggcctc gaaggctcgg
     2581 cctacttgct cgcccagcac gcggcacagc ggcgttttga acgactcgac caccgtgacc
     2641 tccacgtcgt aatggcgcag cgaggctgcc acctcgcagc caatgaagcc catgccgacg
     2701 agtaccgccc gccgaccttt cacgctttcc gcacggatga ggtcagcgtc agccacagtg
     2761 cgcaggtcgt aaatacgctc caattccata ccggggagac ggaacctgcg gttgcgcaca
     2821 cctgtcgcga ctaacacctt atcatagtgt acacgatctc cgtcctccag ctcgaccacc
     2881 tgctcgcgcg ggtccacccg cgttgcccgt acgcccaggc gtgtctggat cgtgtgttct
     2941 gcgtaaaagg tgagcggctt gagcaaggcc tcctcgaacg gcgccttgcc ctggagatat
     3001 tccttggaga gcacaggccg ttcgtagggc aggtgcggct ccgcgccaat gagcacgatc
     3061 tcgccatcga agccctcctc tcggagggta atggcggcag tgcctccggc caggttggcc
     3121 ccgacaatca caaattgctg ctgcatagga cctttctcac tttcattcga ttggctgatc
     3181 cgcgcgatca gtctcgtcgt ccgtcagtcc tcgccttcat cgacgtgttc gggaatggcg
     3241 cctgcttcag ggacctccac gcagatcgtc ccgttctcga tgcgcac
//
LOCUS       DASZAK010000645         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_265858, whole genome shotgun sequence.
ACCESSION   DASZAK010000645 DASZAK010000000
VERSION     DASZAK010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265858"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VGT44_11345"
     CDS             <1..445
                     /locus_tag="VGT44_11345"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEV2581434.1"
                     /translation="SWRQDIAGVDEATRGLAGLGPGLTPSGDDTLAGFISIMALLSPQ
                     LSADGAFREHMARTIAAIAGPRTTVLSAVLLAHAAFGEVAEQLSTLLLALSLSAEASE
                     IVLNAADRALAFGASSGGDTLLGVLLGLRVLEGEIDQSGGYQWPF"
     gene            433..2052
                     /gene="fdrA"
                     /locus_tag="VGT44_11350"
     CDS             433..2052
                     /gene="fdrA"
                     /locus_tag="VGT44_11350"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HEV2581435.1"
                     /translation="MAILALLKRNTYYDSITLMSVAQAAKDLPGVEDTGAIMATEVNL
                     ELLFNANLMPTTFLAEQQTPPGPEDLLIVVRATDDVHAAAALAAAEERLTSRGDRGAP
                     SSAETLPSRSLELALKRDEAANLAVISVAGEHAWLEAEQALRHGLHVFLFSDNVPVEQ
                     EQRLKALAINKGLLMMGPDCGTAIINGIGLGFSNVVSRGSIGIVGASGTGMQQLICLI
                     AAAGMGVSQAIGTGGRDLSEAIGGVMMRSGLELLANDEQTEVIALVSKPPAERVAREI
                     LAAAQITKPVVVVFLGAAPERFAAVSGKAHLARTLTEGAELAVSLAGGDRARVPGKYT
                     LDTYDLEAERAKLEIARVASKAPQSYIRALYSGGTLCDEAMLLLSEQVGAVASNIPLR
                     AEWALAPGETFRGHTALDLGSDEFTRGRPHPMIDPTLRLQYLDRAAEDLETAVILLDV
                     VLGFSAQQNPAAVYAPAIIQAREKAKDAERSLPVIISLCGTEGDPQRLSMQRTALEAA
                     GALVYESNEMAARAAAALVAKETTQETKRRE"
BASE COUNT          491 a          584 c          665 g          430 t
ORIGIN      
        1 aagctggcgg caagatatcg caggagtcga tgaagcgaca cgtggactgg ccggtctcgg
       61 accgggactg acaccatccg gtgacgacac gctggctggt ttcatctcca tcatggcgct
      121 gctcagccca caactcagcg cagacggtgc atttcgcgaa cacatggcaa ggactatagc
      181 cgcaatcgca gggccacgta ctacagtact gagcgccgtt ctgctggcgc atgccgcttt
      241 tggcgaagta gccgaacaat tgagcacgtt attactggcc ctctcgctat ctgctgaggc
      301 atccgagatt gtactgaatg cagcggatcg cgccctggcc tttggcgcaa gctccggtgg
      361 cgataccttg ctgggtgtac tgctgggctt gcgagtactg gaaggtgaaa tcgatcaaag
      421 tggaggttat caatggccat tctagcgcta ctcaagcgaa atacgtacta tgattcgatt
      481 actctgatgt ctgtggcgca ggcagctaag gacctacccg gcgtagaaga taccggcgcg
      541 attatggcga cagaagtgaa cctcgagcta cttttcaacg ccaatttaat gccgactaca
      601 ttccttgctg agcagcagac gccgcctggc ccagaggatc tgctgatcgt ggtgcgcgct
      661 acagatgacg tacatgctgc cgcagcattg gccgctgcag aagaacggct aacttcacga
      721 ggcgatcgtg gcgctcctag cagcgcagaa acgctgccat cacgctcatt ggagttggcg
      781 ctaaagcgcg atgaagctgc taacctggcg gtgatctccg tggcaggcga gcatgcctgg
      841 ctcgaagcag agcaggcatt gcgacacgga ctgcatgtct tcctcttcag tgacaatgtg
      901 ccagttgagc aggagcagcg gctcaaggct ctggccatca acaaaggact tctaatgatg
      961 ggccctgact gtggaacggc aatcataaat ggcatagggc ttggctttag caatgtggta
     1021 tcacgcggct ccataggtat tgtcggagca tcgggcacag gtatgcagca acttatctgc
     1081 ttgattgccg cagcgggtat gggggtttcc caggctatcg ggaccggtgg gcgcgatctg
     1141 tctgaagcca tcggcggcgt catgatgcgc agcggacttg aattactggc aaatgacgaa
     1201 caaacagagg tgatcgccct ggtctctaag ccaccggctg agcgcgttgc tcgcgagatt
     1261 ctggcagcag cgcagattac caaaccggta gtggtcgtct tcctgggagc agcgccggag
     1321 cgattcgcgg ccgtctccgg aaaagctcac ctggcaagga cgcttaccga gggtgctgaa
     1381 ctggcagtct cgctggcggg tggtgatcgc gctcgagtgc cgggcaagta cacacttgat
     1441 acgtatgatc ttgaggcaga acgcgcgaag ctggagattg caagggtcgc ctctaaagcg
     1501 ccacagagtt atatccgcgc actctattcc gggggcaccc tgtgtgacga ggcgatgctg
     1561 ctgctcagcg aacaggtggg cgccgtcgca tcaaatatac ctctacgcgc ggagtgggcg
     1621 ctggcgcctg gcgagacgtt tcgcggccac acagcgctgg acctgggttc cgacgaattc
     1681 acacgaggca gaccacatcc aatgattgac ccaacattgc gcttgcaata cctggaccgc
     1741 gctgcggaag acctggagac ggcggtaatc ttgttggatg tcgtcctggg gttctccgcg
     1801 cagcagaatc cagcggcggt gtatgccccg gccattatcc aggctcgcga aaaagcaaaa
     1861 gacgccgaac gatcgctgcc cgtgattatc tcactgtgtg gaaccgaggg cgatccacaa
     1921 cgactttcga tgcagcgtac ggcgctcgag gctgcaggtg cgctggtgta tgaaagcaat
     1981 gagatggcgg caagagctgc agcagcatta gtggcaaagg agaccacgca agagacgaaa
     2041 aggagagagt aatgccgcaa aaagtacaga gactgagtta tttttccgtc atgatgtacc
     2101 acttgacggg agagcgggcc tacagaggca gcgcagatag cccggcgggc ttcctcaagc
     2161 tcaccacaac
//
LOCUS       DASZAK010000646         6065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_443686, whole genome shotgun sequence.
ACCESSION   DASZAK010000646 DASZAK010000000
VERSION     DASZAK010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443686"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2170)
                     /locus_tag="VGT44_11355"
     CDS             complement(<1..2170)
                     /locus_tag="VGT44_11355"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2581436.1"
                     /translation="MDYQLFLQRLRELPIEEGRDYIRERLAELTDHQTTGELMAEEAL
                     RILYTPFPSLKIAELLIFYGEITGHLSSHALGLKAKGNALVQIGHHQAAIDALDAGGD
                     EFLRMGDEKSWARTRISWVLACGWLGRVEEALKVADAARKVFLRLGEPYWTCVIDNNV
                     AVILGNVGRNEEAVKLYEKMLTIYPTVTSQSKAFIQRSIAIARKNQGNRLFWLGEFEQ
                     AYNLHQQALASFNILGEKDLAVRAEINMADMDYTQGYYGSALRFYYHARELLMQRDEV
                     NALLLAELKTSMANCLVKLNRLQEACLVSEEAIEVYRGIGMSQNAGNALHQHARTLVA
                     SGKLKEALTILDEAWELFEHGGFEPYAYLARLQQAEILLEMGSAAEGHDLAQTIRSYF
                     DAQNLMAYTVRSSLVMVGSMMEEARKALIERKEQPESDLEEAICLCKETLSQARQHNL
                     QEEVYKVHYLMGKIYALQNNLARTSRHYQIAIAQIERILDNLVHDLSPSFLHTTWSVY
                     EEMIALCLEQGQNEKAFSYLERVRSLTLRQHLHNVKSSFNKASRQEENLSLEALRTQM
                     ELKDWQERYHNYSTLLAEFDASVSPTLDKTVVEKQLKHAEEKVSELFEHLHLHQIDID
                     RDGQRSQKRVKTKERSLNTQEIDPGQLRQHLSPEQLLLTYYLHNDRLVIFALTTDSMI
                     TYVNPEGSQEVEHLLRLLLHAHLETDGWPDPQRPPQQAIRR"
     gene            complement(2195..2974)
                     /locus_tag="VGT44_11360"
     CDS             complement(2195..2974)
                     /locus_tag="VGT44_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581437.1"
                     /translation="MQDIAPSDEDLIAYALDGEALSQEAQAHLDQCDICQQRLKRYQH
                     THNALVSQLYRVLCPDAADLNYYSAGGLSEERRQTIAAHLLDCPLCQRDVEEARAYLR
                     EQPVELFAPFSESVLSSPRALARRIYATLVARPQMQFVLRSDAQPSAWPRQYKADAVD
                     LSLHLSRTSGGEHMLIGILTSSDPFEDVEALEGIEAELFVAPLPAMANGHTPTTTPLL
                     RTRVDDLGNVVFKPVPTGNYVMILRLPGREVVIEGLSIEET"
     gene            complement(3096..3800)
                     /locus_tag="VGT44_11365"
     CDS             complement(3096..3800)
                     /locus_tag="VGT44_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581438.1"
                     /translation="MSIEMEDIPDQMTLAALGERCQREIANYRKGLPYDDRYCLEIFH
                     RAILKGDEQAWAWLVDSYSRMVISWLHKHPRREEALRYEPDEDYYVSYTFSRFWQATR
                     NQSLKFDTLAAALHYLKLTLGAAVSDTLRSHARPVERLPEPGMGFPEEPAAEDDTDDG
                     RGLWEVINSLLPTKRQKQVAYLIIHCGLKPREIMQLFPGHFSNVKEIYKTYRNVLELL
                     ERNKDQLRWRLGDEEE"
     gene            4299..5030
                     /locus_tag="VGT44_11370"
     CDS             4299..5030
                     /locus_tag="VGT44_11370"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2581439.1"
                     /translation="MKKRILLGVDAPLSPATRQALRAISAFVVESTSQISLVLLHVIP
                     IPTITSPTFGVYGGHSQSLEFTNEQRERGELALRMARALLQDEGIPSHQIELMLRQGT
                     PTEEVVKVAKELPADMIVIGSRGNSPRQRLRRIFAGSVSRRILTLASCPVTIVVPPAP
                     TPHQHPHDLVKWYETSITHYLAEHTGDLTVFTPQEVTRTFAPPYKKEPGRKERAAAIL
                     ALEQLASSGILCRHDVKGELRYVND"
     gene            complement(5027..>6065)
                     /locus_tag="VGT44_11375"
     CDS             complement(5027..>6065)
                     /locus_tag="VGT44_11375"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2581440.1"
                     /translation="VSGLAPAWVARVNGYLGFTPTVVNNVLYITGGGPTLYAIDTTTG
                     TVIWKTSPQPMRYASYVYSPTVVNGIVYLASDFGALGAFDARTGKLLWSFSSESAASS
                     SGEDSPTFVNGMIYFGSHSGSVYALDARTGRVVWSTVTGGYTGVAVANGVVYAASNTQ
                     LYALSASTGATLWTGPVGAKRTVEVAPVVGSGMVIVTTDDAVVLAFAANGCGQPVCAP
                     RWENDTQQKVWASPAIAYGVVYLSTLYRDFNGGNLVALDAGTGKFLWLAKTDSLYAAP
                     VVANGVVYAINFYGAAYAFNAAGCGNGTCFSLWQSNFFDSNDGIDYSITIVNGQVYIC
                     TPNGSIHALRV"
BASE COUNT         1379 a         1746 c         1529 g         1411 t
ORIGIN      
        1 aacggcgtat ggcttgttga ggaggacgct gtggatcggg ccatccgtcc gtttctaaat
       61 gtgcatgtaa taataaacgt aaaagatgct ctacttcctg actcccttca ggatttacat
      121 acgttatcat gctatcagtg gttagagcaa aaataacaag cctgtcatta tgcaggtaat
      181 atgtaagaag cagttgttca ggcgagagat gctggcgcaa ttgtcccgga tcaatttcct
      241 gcgtgttgag cgatcgttct tttgtcttca cccgtttttg agagcgttgg ccatctctat
      301 ctatatctat ctgatgaaga tggagatgtt caaatagttc gcttactttc tcttcagcat
      361 gcttcagttg tttctctacg actgtcttat cgagtgtagg cgataccgag gcatcaaatt
      421 ccgcaagcaa ggtactataa ttatgatatc tctcctgcca gtcctttagc tccatctgcg
      481 ttcgcagcgc ttccagagaa agattttctt cctgcctgct cgctttgtta aaagaggact
      541 tcacattgtg aagatgctgg cgcagcgtaa gcgaacgcac ccgttcgaga taactgaatg
      601 ccttttcatt ctgcccctgc tccaggcaca acgctatcat ctcttcgtag acagaccagg
      661 tcgtatgtag aaaagaaggt gaaagatcat gtacgagatt gtcgaggata cgttcgatct
      721 gagcaatagc aatctggtaa tgcctggaag tcctggcaag attattttga agagcgtata
      781 tttttcccat taggtaatga actttataga cctcttcttg caaattgtgc tgacgcgcct
      841 gcgacagggt ttccttacac aggcaaatag cttcctccag atcactctcc ggctgctctt
      901 ttcgctctat taacgccttc cgcgcctctt ccatcatcga gcctaccatc accaggcttg
      961 agcgaacggt atacgccatc aaattctgcg catcaaaata gctcctaata gtttgagcca
     1021 gatcatgccc ctcagcagca gaacccatct ctaacaaaat ttcggcctgt tgtaatctgg
     1081 caagataagc atatggctca aagccaccat gttcaaaaag ctcccatgct tcatcgagga
     1141 tagttagcgc ttccttcaat ttaccagaag ctactaatgt tctggcatgt tgatgtaacg
     1201 cattgcctgc attctgagac atcccaatcc ctcgatagac ctcaatcgcc tcttcagata
     1261 caagacatgc ttcttgcaat cgattaagtt tcaccaggca atttgccata gaggttttga
     1321 gctcagctaa taatagagca ttaacttcat cacgctgcat cagcaattcg cgagcatggt
     1381 agtagaacct tagagcagag ccatagtagc cctgtgtata atccatgtcc gccatattaa
     1441 tttccgctcg aacagccaga tctttttcac caagtatatt aaagctggcg agggcctgct
     1501 gatgtaggtt ataagcttgt tcaaactcac caagccagaa cagacgattt ccctggtttt
     1561 tccgggcaat agctatagag cgctgaataa atgccttgct ttggcttgtc acagtaggat
     1621 agatagtcaa cattttttca tacagcttga ccgcttcttc atttctcccc acattaccca
     1681 gtatcacagc aacattatta tcgatgacgc aggtccaata tggttcacca agtcgcaaga
     1741 acactttgcg agcagcatca gcaactttca gcgcttcttc gacacgtccc agccagccgc
     1801 aggccagtac ccagctaatg cgggtgcgcg cccagctttt ctcgtcaccc attcgaagga
     1861 attcatctcc cccggcatcc agcgcatcta ttgcggcttg atgatggccg atctgtacca
     1921 gggcattccc cttcgccttc aaccccagcg cgtgcgaaga gagatgcccc gtaatctccc
     1981 catagaaaat cagcagttcg gcaatcttga gcgaggggaa aggggtatag aggatgcgca
     2041 gcgcctcttc cgccatcaat tccccggtgg tctggtggtc ggtgagttct gcgaggcgtt
     2101 cccggatgta gtcgcggccc tcttcgatgg gcaattcacg caggcgttgt aaaaatagct
     2161 ggtaatccat tgccgacgat acggctcctt gctcttacgt ttcttcgata gataacccct
     2221 cgatcacgac ttcgcgtcca ggcaggcgca gaatcatgac atagttgccg gtgggaacag
     2281 gtttgaagac gacgttgccc aggtcgtcta cccgcgtgcg cagcaggggt gtggtagtcg
     2341 gtgtatgccc attggccatg gctggcaaag gggcgacaaa tagctcggcc tcgatgcctt
     2401 cgagcgcttc tacatcctca aatggatcgg aactggtcag aatgccgatc agcatatgct
     2461 caccgccgct cgtgcgagag agatgcaggg aaagatcgac cgcatcggcc ttgtactggc
     2521 gcggccaggc gctcggctgc gcgtcgctgc gcagcacgaa ttgcatctgc gggcgcgcga
     2581 ccagcgtggc gtaaatgcgg cgcgccagcg cgcgcggcga ggacagcaca gactcagaga
     2641 acggagcaaa caattcgacg ggctgttccc gcaaataagc gcgggcttct tcgacatccc
     2701 tttgacacag tgggcaatcc agcagatgcg cagcaatcgt ctggcgtctt tcctccgaaa
     2761 gaccacctgc gctgtaatag ttcaaatccg ccgcgtctgg acacaggacg cgatagagct
     2821 gcgacacaag cgcgttatgc gtatgctggt agcgcttcag acgctgttgg cagatatcac
     2881 actgatccag gtgagcctgg gcctcttgcg ataatgcctc gccgtcgagc gcataagcaa
     2941 tcagatcctc gtcgctgggt gcaatgtctt gcaaacagtc cgtcattcgt tcaacctcat
     3001 ttctatgctg gcaccggcta taaatcccat cccctattca tcacattcgc tctataaacg
     3061 atgcacccgg cgttttttgg ggattggata tcgcgttact cttcctcgtc accgagccgc
     3121 cagcgtaact ggtccttatt gcgctccaac agctcgagga cgttgcgata cgttttataa
     3181 atttccttga cgttgctgaa atggccaggg aacaactgca tgatctcgcg cggcttcagc
     3241 ccgcagtgaa tgatcaaata ggccacctgc ttttggcgct tcgtcggcag caggctgttg
     3301 atgacctccc acaggccgcg cccatcatcc gtatcatcct ccgccgccgg ttcttccggg
     3361 aagcccatgc ccggctccgg cagccgctcg accgggcgcg cgtgcgaccg cagcgtatcc
     3421 gacaccgccg ctcccagggt cagcttcaga tagtgcagcg cggcagccag tgtatcgaac
     3481 ttgagcgact ggtttctggt cgcctgccag aaacgcgaga aggtgtacga tacataataa
     3541 tcttcgtccg gctcgtaacg caaggcttct tcgcggcgtg gatgcttgtg cagccagctg
     3601 atgaccatgc gcgaatagct gtccaccagc caggcccagg cttgctcatc gcctttcagg
     3661 atggctcgat gaaaaatttc caggcagtag cggtcgtcgt agggcagtcc cttccggtag
     3721 ttggcaatct cgcgctggca gcgctctcct agcgcggcca gggtcatttg gtcgggtatg
     3781 tcttccatct cgatactcat cacattcgac ctgctttctt tagagaaccc ttgcaggccc
     3841 cctcaaccgt aacataccag ccagcgggcg ctccccggta tgttactacg cgcgctctca
     3901 tgctcatgcc acaaaaactg ttctgttact acagttagac gtaatacatg gacgatgttc
     3961 aaatcgcggg ctatctctac agttgggtcg ctttaattct tcagtatcaa caatagctta
     4021 acgcaatacc atatactcgt caatgcggtt tcaagcacga atcgtgtgaa atacttcaca
     4081 gttgcctgtg cgatgtcgtc tgctcacaac atacccgata tgcagagaat gcgcatgtgt
     4141 catatcaccc atttccagag cttgtcgcga cgcccaaaac tatgtatact attggcgaac
     4201 aggtgtgttt tcattgcccg cgaaccggca aacaattggc gtgtaggaaa cgatagggaa
     4261 cagacgcaag actcaacgtt cacgcggcgg gaactgccat gaagaagcgc atcttactgg
     4321 gggttgacgc ccctctctca cctgccacgc ggcaggcatt gcgcgccatc agcgcatttg
     4381 tcgttgagag tacttcccag atatctctcg tcctgctgca tgtcatcccc attcccacca
     4441 tcacctcacc aaccttcggc gtttacggcg gccattctca atccctcgaa ttcaccaacg
     4501 agcagcgcga acgcggcgaa ttggccctgc gtatggcgcg cgccctcttg caggatgaag
     4561 gcattccctc gcatcaaatc gaattgatgc tgcgccaggg aacgcccaca gaagaagtcg
     4621 tcaaagtcgc gaaggaactg cccgccgaca tgatcgtgat cggcagtcgc ggcaacagcc
     4681 cgcgccagcg actgcgccgt atcttcgcag gcagcgtttc tcgtcgcatt ttgaccctgg
     4741 caagttgccc agtgaccatc gtcgttccgc ccgctcccac accgcatcaa cacccccacg
     4801 acctcgtgaa atggtacgaa acatccatca cacattacct cgcagaacat accggcgacc
     4861 tgaccgtctt cacgccccag gaagtcacgc gcaccttcgc tccaccgtac aagaaagagc
     4921 cgggccgcaa agaacgcgcc gccgccatcc tggcgctcga acagcttgcc agcagcggca
     4981 tcctctgccg ccacgatgtc aaaggcgaac tgcgctatgt caacgactaa acgcgcaagg
     5041 cgtgaattga gccgtttggc gtgcagatat agacctgccc attgacgatt gtgatcgagt
     5101 aatcgatgcc gtcgttgcta tcgaagaagt tgctctgcca gagcgaaaag caggtgccgt
     5161 tgccgcaccc ggcagcattg aaggcgtagg ccgcaccgta gaaattgatg gcatacacaa
     5221 cgccatttgc tactacagga gccgcgtaaa ggctgtcggt tttcgcgagc cacaagaact
     5281 tgcccgtgcc tgcatcgagc gcgacaagat tgccgccgtt gaaatccctg taaagcgtgg
     5341 agaggtacac gacaccataa gcaatcgctg gtgaggccca tactttttgt tgggtgtcat
     5401 tttcccagcg cggcgcgcaa accggctgcc cgcagccgtt ggctgcgaag gcgagcacca
     5461 ccgcgtcgtc cgtggtgacg atgaccatgc cgctgcccac gacgggggcg acctcaacag
     5521 ttctcttcgc cccgaccggg cctgtccaca gggtggctcc cgtgctggcg ctcagcgcgt
     5581 agagctgcgt gttggaggcg gcgtagacca cgccgttcgc gacggctact cctgtatatc
     5641 ctcctgtgac agtactccat accaccctcc ctgtcctggc atccagggcg tagacgcttc
     5701 cactgtgcga gccaaagtag atcatgccgt tgacaaaggt aggcgaatcc tcgccggatg
     5761 agcttgcggc gctttcgctg gagaaagacc agaggagctt gcccgtgcgc gcatcgaatg
     5821 ctccaagagc gccaaaatca gaggcaagat acacaatacc gttcaccacg gtcggcgagt
     5881 agacgtagct ggcataccgc atcggttggg gcgaggtctt ccagatcact gttcccgtcg
     5941 tggtgtcgat agcgtagagc gtcggacctc ctccggtgat gtaaaggacg ttgttgacga
     6001 ctgtgggggt gaacccgaga tacccattga cacgcgctac ccaggcagga gccaggccgg
     6061 agacg
//
LOCUS       DASZAK010000647         3016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_372520, whole genome shotgun sequence.
ACCESSION   DASZAK010000647 DASZAK010000000
VERSION     DASZAK010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372520"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2098)
                     /locus_tag="VGT44_11380"
     CDS             complement(<1..2098)
                     /locus_tag="VGT44_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEV2581441.1"
                     /translation="MHWHPVTPFHLDVAEFEQALTLADAATRRNDQRAQQDALEQADS
                     LYRGELLPGCYDEWILPERDRLRQGHLQVLEQLLRLFEGRGDHVTAIRYAQRLIGLDP
                     LSEDLYRRLMRLFALNNDRASALRVYHTCVTTLQRELGVEPGQATREAYERLMQQETP
                     TIQAIVHQSLLAASPTLIGRKREWEDLQDAWHHATTAEPHFVLVTGEAGVGKSRLAEE
                     FLLWASQQGAVTAKARSYAAEGQLSLAPVTDWLRSDGLRSKLRQLDEVWLTEVVRLLP
                     ELLAEQPDLPHYEPVTEYGQRQRFFEALARAILVVPQPLLLLIDDLQWCDQETLEWLH
                     FLLRFDPMARLLVVGCAREEELPPQHPLRTFLLHLRSTMRVTEIALQPLDAAETVKLA
                     DAVAKRELDLDEGMHLFHETGGYPLFVVEMVRANLSIPADNAADAATLRVSQSERKYL
                     GRVSASPPEADRSHGQPPLDDARTLPPRVHAVLVGRLLQLSASAREFVELAATIGREF
                     TLDLLSTAGNADADSAVRALDELWHKRIVREYGANSYDFTHDKLREVAYGEISAPQRR
                     MLHRRVAQALEALHAGDLDPVSGQIASHYERAGMIEQALPYYQRAAAVAQGVYANEDA
                     ISLLSRSLELLELLPAGSKRDKQELGLQLALAPLYRVTRGWTAPELERVLDRALALCD
                     TVGNDAQRAQTLYGLQS"
     gene            1994..2443
                     /locus_tag="VGT44_11385"
     CDS             1994..2443
                     /locus_tag="VGT44_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581442.1"
                     /translation="MGTLIIASCSRVCESERLFELSDIKVEWRDWVPMQQVSVGREEV
                     FHIRKCLAQLVQQLAQVIARLSLGGIRPEEKSKMLALLGNIAMQHQVSEQGAQAHGIE
                     IGHLLVVVEQAEIAKQSEVKGWYRYDWLPFSSQAQAMAEWPALGTCT"
BASE COUNT          644 a          862 c          864 g          646 t
ORIGIN      
        1 cggactgcaa tccatagagc gtctgcgccc gctgtgcgtc gttacctacc gtgtcgcaca
       61 acgccagtgc gcgatcgaga acacgctcca gttccggcgc tgtccagcct ctggtcaccc
      121 ggtagagtgg cgccagtgca agttgtaggc cgagctcctg tttgtcgcgt ttcgaaccgg
      181 caggcagcag ctccagcaac tccagactcc tcgagagcag gctgatggca tcctcattcg
      241 cgtacacgcc ttgcgccact gctgccgcac gctgataata ggggagcgcc tgctcgatca
      301 tacccgcgcg ctcgtaatgc gacgcaatct ggccgctgac agggtcgagg tccccggcat
      361 gcaatgcttc caacgcttgc gcgacgcgac ggtgcagcat gcgccgctgt ggcgcactga
      421 tctctccata agcaacctcg cgtagcttat cgtgggtgaa gtcataacta ttcgcgccat
      481 attcgcgcac gatccgtttg tgccagagtt cgtccagcgc gcgtacggcg ctatcggcgt
      541 cggcgttgcc ggcggtgctc aacaggtcga gcgtgaattc gcggccaatg gtcgcggcaa
      601 gttcaacgaa ctcgcgagca gaggccgata gttgaagtag gcgaccgacc agcaccgcgt
      661 gtacgcgtgg cggaagcgtt cgagcatcat caagcggcgg ttgcccgtgg gagcgatcgg
      721 cctctggcgg gctggctgaa actcgtccca ggtacttcct ttccgactga ctgacgcgaa
      781 gcgtagccgc gtcagcggcg ttgtcagccg gaatagacag gttggcccgc accatttcaa
      841 ccacgaacag gggatatccc cctgtctcat gaaacaagtg catcccctcg tccagatcca
      901 actcacgctt tgccacagcg tcggccagtt tcactgtttc cgctgcatct aacggctgga
      961 gcgcgatttc tgtcacacgc atggtgctgc gcagatgcag caggaaggtg cgcagcggat
     1021 gctgtggcgg cagttcttcc tcgcgggcgc acccgacgac gagcagtcgc gccatagggt
     1081 cgaagcgcaa cagaaaatgc agccattcca gcgtttcctg gtcgcaccat tgcaagtcgt
     1141 cgatcaacaa gagtaatggc tgtggtacga caaggatggc gcgtgccagt gcctcaaaga
     1201 agcgctggcg ctgaccatac tcggtcactg gctcgtagtg cggtaaatct ggctgctcag
     1261 ctaacagctc cggcaggagg cgcacgactt cggtgagcca gacctcgtcg agttgcctga
     1321 gcttcgagcg cagaccgtcg cttctgagcc aatccgtcac tggtgccagc gagagttggc
     1381 cctcagcggc ataggagcgc gccttcgcgg tgacggcacc ctgctggctc gcccacagca
     1441 ggaattcctc ggccagccgt gatttgccca cgcccgcttc cccggtcacg aggacgaagt
     1501 gtggctcagc agtggtggca tgatgccagg catcttgcag atcctcccac tcccgctttc
     1561 gtccgatgag cgttggcgaa gcggcaagca gcgattgatg tacgatggct tgtatcgtcg
     1621 gcgtctcttg ctgcatgagt cgctcgtaag cttctcgagt cgcctgacca ggttcgacgc
     1681 ccaattcgcg ctgtaaggtg gtgacacagg tgtgatacac ccgcaacgcg ctggcgcggt
     1741 cgttattgag cgcgaacaag cgcatcagac ggcgatagag gtcttcggag agagggtcga
     1801 gtccgatcaa gcgctgggca taccggatgg cggtgacgtg gtcgccccgc ccctcaaaga
     1861 ggcgtagtag ttgttcaagg acctgcaaat ggccctgacg gagtcggtcc cgctcaggca
     1921 agatccattc atcatagcag ccaggcagta attctccgcg atacaggcta tctgcttgct
     1981 caagagcatc ttgttgggca cgctgatcat tgcgtcgtgt agccgcgtct gcgagagtga
     2041 gcgcctgttc gaactcagcg acatcaaggt ggaatggcgt gactgggtgc caatgcaaca
     2101 ggtgagcgtc ggcagagagg aagtgttcca catccggaaa tgcctggcgc aactggtgca
     2161 gcagttggcg caagttattg cgcgcctgag cctcggtggc atcaggccag aagagaaaag
     2221 caagatgctg gcgctgttgg ggaacatcgc gatgcaacac caggtaagtg agcaaggagc
     2281 gcaggcgcat ggtattgaga ttggtcacct gctggtcgtt gtagagcagg ctgaaatcgc
     2341 caagcaatcg gaggtgaagg gttggtatcg ttatgattgg ctacctttct cctctcaggc
     2401 tcaagcgatg gcagagtggc cagctcttgg aacatgtaca taacactacg aaaatcatcg
     2461 aaaccatcta ctggcgatgc gtccgatcaa cttcagcttg caacaattgt tcctgatgtt
     2521 cttactctat catatcatgc tctttttacc aataccccct ttctctgtgc atgttcgcat
     2581 ggaatgccgc ttaggcgacc gcatcatttc accatatcca ctgaatgcgg ggaggggaac
     2641 tgaatacgtt catgttcgga agtacagcta ctctcgcaag ctttccctgc tctcattttg
     2701 tcatggcaag ttgacaaaat gacaagcaca cttgacaaag caacaattat ctcttatact
     2761 aagacaagga aacttgacaa aaagatatgc atgctttaca tattaccgga tgcttagcat
     2821 ccagaaagga tactttctgc tatgaaccag tcagcatcaa aaacatggac acgagaattt
     2881 ctcacggcca tggcagccta tgtgattgtt ctgccgatct gcattgtgtt gatcaatgcc
     2941 agcccgctct cagcatggtg gcgtatccca ctggcactga tcccagtgat tcccatcctc
     3001 ttcgccatga gagcat
//
LOCUS       DASZAK010000648         6185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_299918, whole genome shotgun sequence.
ACCESSION   DASZAK010000648 DASZAK010000000
VERSION     DASZAK010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299918"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2608
                     /locus_tag="VGT44_11390"
     CDS             <1..2608
                     /locus_tag="VGT44_11390"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2581443.1"
                     /translation="TALQEILPADLYKKIESPLREATIDGAIRHFDDLPYEGFLEMRG
                     RTYWHIAIVPTVRERIDADLLSKLPASNDAPALSGSLLLVTIEDVTDKVRAQMHIDAI
                     HYISSAIARGDVLPQVMDHVLQAVQELVGSTRCAILLLETPVDEGNARQAVSPLQAGE
                     LHVLPRISIASQKGLHLSSRGWNPQLGEHVLLGKVVQTRHSLVIPNTNLHPEIEFPLL
                     DDEGVARRPGSVLCVPIFEPAPASDAREGVSRGAILGTIEVYHRRVRGLPAEEVELLE
                     QFAQQVGLAIQNARLFSHNAMLTQDARREVRQRENIMQAIPDGIIIYDARWRVSEMNS
                     AIRWLLGWTDEIIGLSIHEALARSSAIYLDRSPSIEEMVADLERSPQEPSVDEFKMIG
                     ANGKLYAMRRSKAPISDDEGRIFAYIVVYHDVTDQVTARESIEAQVIARTAELAQRNK
                     ALEDAREALAMEHAQLELLLARLPSGVVLVSSGDRRIINCNPQATRLLGLMRASGLDT
                     PYRSPITGDISASDNMLGADIEELLRGVEAYGPNGALLPYEEQPIYLALRQGRAGEAE
                     IHITRDDNQPLYLLVSAAPLRANDGSITNVVLVLQDITIVKALERAREDFFTTMAHEL
                     KTPLANIRAHLSALQVSDYPLSNDEQMEYLKTADEQVDRLVGMINHFLDASRVEAGAL
                     RLEMEPVLIPELFEDLEERLEALIASSHRSLEVFISPDIPAAHADYEMIMSVLINLLS
                     NAFRYAPEGDAVRLEAETIYTPGDSQPTGVKLRVIDRGIGITPERQAELFTRFSTFAA
                     SRRPDPGRPGQPALRRSGSGRWSQATGLGLYISRGIIEAHGSTLRLHSAPGEGTTFAF
                     QLGVASLVKHEV"
     gene            2611..3363
                     /locus_tag="VGT44_11395"
     CDS             2611..3363
                     /locus_tag="VGT44_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2581444.1"
                     /translation="MATTNRKIRILCVEDDPQMRTFLMNQLRVRGHQVQAVQDGAQAI
                     DQAAIFQPELILLDLALPSLDGLAFLRRLREWSQSYVIVLSAHDEESVKIKALDLGAD
                     DYLTKPFGMEELLAHIRAAMRRADELERLRGGGSGSDSSNPVYHSGGSNGLIVDFAHR
                     QVRSGGREVHLTPIEYALLSELTRNPGKVLTHRELLRRVWGAEYVAENTYLRTFIRHL
                     RRKLEPDPTKPRFLLNEPGVGYFVPQPDAVEA"
     gene            complement(3368..4345)
                     /gene="hemB"
                     /locus_tag="VGT44_11400"
     CDS             complement(3368..4345)
                     /gene="hemB"
                     /locus_tag="VGT44_11400"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEV2581445.1"
                     /translation="MSTFPTVRMRRIRQNEQLRGLVRETHLNVEQLIYPLFIAEGLSE
                     PHPIAAMPGIMQWPLEHLGREMERVASLGIPAVLLFGIPTEKDEVGSQAYAEQGIIQQ
                     AIRRIKAETPGVLVITDVCLCEYTSHGHCGVIRNGEVQNDESLKLLANMALSHVEAGA
                     DMVAPSDMMDGRVGAIRHALDEQGFSGLPIMAYSAKYASGFYGPFREAAGSAPQFGDR
                     RSYQMDPANAREALREIDLDIAEGADIVMVKPALAYLDVIRRAREHCDLPIAAYNVSG
                     EYAMIKATAMQGWIDERRIVMEVLTGIRRAGADMIITYFAPDVAQWLRE"
     gene            complement(4350..4655)
                     /locus_tag="VGT44_11405"
     CDS             complement(4350..4655)
                     /locus_tag="VGT44_11405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581446.1"
                     /translation="MKKTFVTIFIPGTNWVAGKTSREQPYWTEHATFMDALFEDGMVI
                     MGGPFADYSSILVIIEASDEGSVRDLFKHDPFIVHEILHLSSVHEWLVFLDTRKKGQ"
     gene            complement(4681..5970)
                     /gene="hemL"
                     /locus_tag="VGT44_11410"
     CDS             complement(4681..5970)
                     /gene="hemL"
                     /locus_tag="VGT44_11410"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEV2581447.1"
                     /translation="MNLSRSHTLFAEAQELLPGGVNSPVRAFRGVGGEPVFIDHAHGP
                     YLFDVDGNRYLDYIQSWGPMILGHVYPAVVEAVTQASQRGLSFGAPTAAESELAKLVI
                     ECVPSIEMVRFVNSGTEATMSALRLARAFTGCSKIVKFSGCYHGHADMLLVQAGSGVA
                     TMGLPDSPGVPVEATQHTLLAPYNNSAAVEALFARHPGEIAAIIVEPVAANMGLVLPM
                     PGFLERLRELADLNGALLIFDEVITGFRVALGGMQGRAGVLPDLTCLGKIVGGGLPVG
                     AYGGRREIMQQVAPLGPMYQAGTLSGNPLAMAAGIATLRELRKPDRYEELERKGQLLE
                     EGIQRAIKQAGINVQFARLGSLFGLFFTSQPVIDYASAKTSDTARYARYFWKMLERGV
                     YLPPSQFETCFISLALEDAMIEETIEAVKVALHESAP"
     gene            complement(5967..>6185)
                     /locus_tag="VGT44_11415"
     CDS             complement(5967..>6185)
                     /locus_tag="VGT44_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEV2581448.1"
                     /translation="IAYPSVKLNTTYSFGLDDQEFVVAFETDEIGDFLDLVQELRETE
                     ASMFTLRDTPMFTCVAQPFDEILEAIGA"
BASE COUNT         1274 a         1950 c         1773 g         1188 t
ORIGIN      
        1 cactgcgctg caagagatcc ttcccgccga cctttacaag aaaattgagt cgcctctgcg
       61 cgaggctaca atcgatggcg caatacgcca tttcgacgac ctgccctacg agggtttcct
      121 tgagatgcgc ggacgcactt actggcatat tgccatcgtg ccgaccgtgc gggaacgcat
      181 cgatgccgac ctgctgagca agctgcccgc atcaaacgac gcaccggcgc tctccggatc
      241 gcttttgctc gtgaccatcg aggatgtcac ggacaaagtg cgcgcgcaga tgcatatcga
      301 cgctattcac tacatctcct cggccattgc caggggagac gtgctgcccc aggttatgga
      361 tcacgtcctc caggcggtgc aagaactcgt cgggtcgacg cgctgcgcca tcctcctgct
      421 cgaaactcct gttgacgaag gaaacgcccg ccaggcagta tctcccctac aagccgggga
      481 attgcatgtg ttaccacgta tctcaatcgc ttcgcaaaag ggactgcacc ttagctcgcg
      541 cggctggaat ccgcagcttg gggaacatgt cttgcttggc aaggtcgtac aaacgcgcca
      601 ctcgctggtg attccgaaca caaacctgca tcctgaaatc gaatttcctc tgctcgacga
      661 cgagggcgtc gctcgccgac ccggctcggt gctatgtgtg ccaatttttg agcctgcgcc
      721 ggcgagcgac gcgcgcgagg gcgtatcgcg cggggccatc ctgggaacca tcgaggtcta
      781 tcaccgccgt gtgcgcggtc tgcccgccga ggaagtggag ctattggagc agttcgcgca
      841 gcaggtcggc ctggccattc agaacgcgcg actcttctct cacaatgcta tgctgaccca
      901 ggatgcccgg cgtgaggtgc gccaacgcga gaacatcatg caggccatcc ccgatggcat
      961 catcatctac gacgcacgct ggcgtgtctc agaaatgaac agcgccattc gctggctcct
     1021 cggctggacg gacgagatca tcggcctctc aattcacgaa gccctggcgc gcagcagcgc
     1081 gatctacctg gatcgttcgc cctctatcga agagatggtc gccgacctcg aacgctctcc
     1141 ccaggaaccg agcgttgacg agttcaagat gatcggcgcg aatggcaaac tctacgccat
     1201 gcgccgctcg aaagcgccca ttagcgatga cgagggtcgc attttcgcct atattgtggt
     1261 ctaccatgac gtgaccgacc aggtcacggc gcgcgaatcg attgaggcgc aggtcatcgc
     1321 gcgcaccgcc gaactggccc agcgcaacaa agcgctggag gatgcgcggg aagcgttggc
     1381 gatggaacat gcccagctcg agctgctgct ggcgcgcctg ccttccggcg tcgtgctggt
     1441 ctcgtcaggc gaccgacgta tcatcaactg caatccccag gccacacgac tgctcggcct
     1501 gatgcgcgct tccggcctgg atacgccata ccgttccccc atcaccggcg acatatctgc
     1561 cagtgacaac atgcttggag cagatatcga agaactcttg cgcggggtag aggcgtatgg
     1621 tccaaacggc gcgctgctgc cctacgagga acaacccatc tacctggcct tgcgccaggg
     1681 cagagccggc gaagcggaaa ttcatattac acgggacgat aaccagccgc tctacctgct
     1741 ggtcagcgcc gcgcccctgc gagcaaacga cgggtcgatt accaatgttg tgctggtcct
     1801 gcaagatatt acaatcgtga aggcgttgga gcgcgcgcgc gaggatttct tcaccaccat
     1861 ggcgcacgaa ctcaagacgc cgctggccaa catccgggcg cacctgagcg ccttacaggt
     1921 cagcgactat cccctctcca acgatgagca gatggagtat ctcaagaccg ccgatgaaca
     1981 ggttgatcgc ctggtgggca tgattaacca cttcctggat gcctcgcgcg tcgaggcggg
     2041 cgcgctgcgg cttgagatgg aacccgtgct gattcccgaa ctctttgaag acctggaaga
     2101 gcgtctcgaa gccctgattg cttcctccca ccgcagcctg gaggtgttca tctcgccaga
     2161 tattccggcg gcgcatgccg attatgaaat gattatgagc gtgctgatca atctgctgag
     2221 caatgccttc cgctatgcgc cagagggaga tgcagtgcga ctcgaagccg aaacgatcta
     2281 tacacctggt gatagtcagc cgacaggcgt caaactgcgc gtcatcgacc ggggaatcgg
     2341 catcacgccc gaacgccagg cggaactctt cacacgcttc agcacctttg ccgcgtcgcg
     2401 ccgtcccgac cctggacgac ctggccagcc cgcgctccgt cgaagcggca gcgggcgctg
     2461 gagccaggca accggtttgg gattgtacat cagtcgtggt atcattgaag cgcatggcag
     2521 cacgcttaga ttgcacagcg cgcctggcga gggaacgacc ttcgccttcc aacttggcgt
     2581 tgccagcctg gtcaagcacg aggtgtaacg atggctacca ccaatcgcaa gattcgcata
     2641 ctctgtgttg aggacgaccc gcagatgcgt acgttcttga tgaaccagct gcgagtcagg
     2701 ggacaccagg tgcaggccgt gcaggacggc gcgcaggcca tcgatcaagc ggccattttt
     2761 cagcccgaac tgatcttgct cgacctggcg ctgccctcgc ttgatggcct ggccttcctg
     2821 cgacgcctgc gcgaatggag ccagtcctac gtgatcgtcc tctcggccca cgatgaagag
     2881 tcggtcaaaa tcaaggcgct cgacctgggc gcggacgact acctgacaaa gccctttggc
     2941 atggaagaat tgctggcgca tattcgcgcc gccatgcgcc gcgccgacga actggaacgg
     3001 ctgcgcggcg gcgggagtgg cagcgatagc tccaaccctg tttatcacag cggcggttcc
     3061 aacggcctga tcgtcgattt cgcgcatcgc caggtgcgtt ccggcgggcg agaagtccac
     3121 ctgacgccca tcgaatacgc cctgctcagc gagctcaccc gcaaccccgg caaagtcctg
     3181 acgcaccgcg aactgctcag gcgcgtctgg ggagccgaat acgtcgccga aaacacctac
     3241 ctgcgcacct tcattcgtca cctgcgccgc aagctcgaac ccgacccgac aaaacctcgc
     3301 ttcctgctca acgaacccgg cgtcggctac ttcgtgcctc agcccgacgc ggtggaggcg
     3361 tgaagaatta ttcccgcaac cactgcgcta catccggcgc aaaataggta atgatcatat
     3421 ccgcgcccgc gcgccgtatg cccgtcagca cctccatcac gatacggcgt tcgtcgatcc
     3481 agccctgcat ggcggtggct tttatcatgg cgtattcgcc gctgacgttg taggccgcga
     3541 tgggcaggtc gcaatgctcg cgcgcgcggc ggattacgtc gaggtaggcc agcgcgggct
     3601 tgaccatcac gatgtcggcc ccttctgcga tatccaggtc gatttcgcgc agcgcttcgc
     3661 gcgcgttggc cgggtccatc tggtacgagc ggcggtcgcc aaactgcgga gcggacccgg
     3721 cggcctcgcg aaacgggccg tagaagcctg atgcatattt ggcggaatag gccatgatcg
     3781 gcaagccgct gaagccctgc tcatccagcg cgtgacggat cgcgcccacg cggccatcca
     3841 tcatatcgga cggcgcaacc atgtccgcgc ccgcctcaac gtgtgacagc gccatattcg
     3901 ccagtaattt cagcgactcg tcgttttgca cctcgccatt gcgtatcacc ccgcagtgtc
     3961 catggctggt atactcacac aggcacacgt cggttatgac cagcacgcct ggcgtttcgg
     4021 ctttgatgcg gcgaatggct tgctgaataa tgccctgctc agcatacgcc tgcgaaccaa
     4081 cttcgtcttt ttcggtggga atgccaaaga gcagcaccgc aggaatgccc aggctggcca
     4141 cgcgctccat ttcgcgccca agatgctcca gcggccactg catgatgccc ggcatggccg
     4201 cgatgggatg cggttcagac agaccttctg cgatgaacag cggatagatg agttgttcga
     4261 cgttcagatg cgtttcgcgc accaggccgc gcagttgctc attttgacgt atgcgccgca
     4321 tacgtacggt gggaaatgta ctcatgctct tattgcccct tctttcgcgt gtccagaaaa
     4381 acgagccatt catggacgct actgaggtgt aatatttcat gcacaatgaa agggtcatgc
     4441 ttgaacaggt cacgcacaga tccctcgtcg gaagcttcaa tgataaccag gatactactg
     4501 taatcagcaa agggaccacc cataatcacc atgccatcct cgaacagcgc atccatgaaa
     4561 gtcgcgtgtt ctgtccagta cggctgttcc cggctggttt tgcctgctac ccagttagtg
     4621 ccaggtataa aaatggtgac aaacgttttt ttcaagcgac tcgtgcctct caatgaatga
     4681 ctaaggcgca ctttcgtgca gtgctacctt gactgcctct attgtctctt caatcatagc
     4741 atcttccagc gcgagcgaga tgaaacaggt ctcgaactgc gagggcggca ggtagacgcc
     4801 gcgttcgagc atcttccaga aatagcgcgc ataacgggcg gtgtccgacg ttttcgcact
     4861 ggcatagtcg atcacaggct gcgaggtgaa aaacaggccg aagagcgaac ccaaccgggc
     4921 gaactgtacg ttgatgcctg cctgctttat ggctcgctgg atgccctctt cgagcaactg
     4981 tcccttgcgc tcaagctctt catagcggtc cggcttgcgc aactcgcgca gtgtagcgat
     5041 gccggcggcc atcgccaggg gattaccgga gagcgtaccg gcctggtaca ttgggccaag
     5101 cggcgccact tgctgcatga tctcgcgacg cccgccatag gctccgaccg gcaggccgcc
     5161 gccgacaatc ttgcccaggc aagtcaggtc gggcagcact cccgcacgcc cctgcatgcc
     5221 tccgagggcg acgcgaaagc cggtgatgac ctcgtcgaag atcagcaatg cgccgttgag
     5281 atcggccagc tcgcgcagtc gctcgagaaa gccgggcata ggcagcacca atcccatatt
     5341 ggccgctacc ggctcaacga tgatggctgc gatctcgcca ggatgtcgag caaagagcgc
     5401 ctcgactgcc gcgctattat tgtagggcgc gagcaaggta tgctgtgttg cctcgaccgg
     5461 cacgccgggg ctgtcgggca atcccatcgt ggcgacgcct gaacccgcct gcaccaacag
     5521 catatcagcg tgaccatgat agcagccact gaacttcacg atcttcgagc agcccgtaaa
     5581 cgcgcgcgcc agccgcagcg cgctcatggt cgcctcggtg ccggaattga cgaaacgcac
     5641 catctctatc gacggcacgc attcgatgac cagcttcgcc agctcgcttt ccgccgccgt
     5701 tggcgcgcca aagctaagcc cgcgctggct ggcctgggtc acggcctcaa ccaccgcagg
     5761 ataaacgtgt cccaggatca tcggccccca ggactggata tagtcgaggt agcggttgcc
     5821 atccacgtca aataaatagg ggccatgggc gtgatcgatg aagaccggct cgcccccaac
     5881 gccgcgaaaa gcgcgcacgg ggctattgac tccccccggc aaaagctcct gcgcctccgc
     5941 gaagagcgtg tgtgatcgcg acaaattcat gcgccgatag cctccaaaat ctcatcgaaa
     6001 ggttgagcca cgcacgtgaa catgggcgta tcgcgcaggg tgaacatact ggcctcggtt
     6061 tctcgtaact cctgcaccag gtcgagaaaa tcaccgattt cgtccgtctc gaaggccacg
     6121 acgaattcct ggtcgtcaag gccgaaggag taggttgtgt tgagcttgac cgaagggtag
     6181 gcgat
//
LOCUS       DASZAK010000649         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_514551, whole genome shotgun sequence.
ACCESSION   DASZAK010000649 DASZAK010000000
VERSION     DASZAK010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514551"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VGT44_11420"
     CDS             <1..871
                     /locus_tag="VGT44_11420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEV2581449.1"
                     /translation="SAIPGTGTSGRVRKDDILAYLDQRQAAPAAPQAPSPAPARVAIA
                     PAAPAAPTFAGQDEELIEPSRMRLAIAEHMVRSKHTSPHATTVVEVDMTNIAKWLERN
                     KEDFKRREGYSISFVPFVMKAVCEGLRKFPYMNASWTEDNKIVLKKRINLGMAVATDA
                     GLVVPTIYDADQFTLAGLAKQVNAVAQRARANKLTIQDMTGSTFVVNNPGTFGTIISV
                     PIINQPHAGILSMDAVVKRPVVIEDDAIAVRSMMYLCLSFDHRINDGAGAGGFLQAAR
                     QKLQSYGREIDVY"
     gene            883..1758
                     /gene="lipA"
                     /locus_tag="VGT44_11425"
     CDS             883..1758
                     /gene="lipA"
                     /locus_tag="VGT44_11425"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEV2581450.1"
                     /translation="MATMIPERRPEWLRVRAPRGDNYDNLKQLMRSKGLHTVCEEARC
                     PNIGECWGYKTATFMILGRVCTRSCGFCAVETGRPIGLDWEEPKRVAEAVQDMGLRHA
                     VVTSVNRDELKDGGAAIFASTIRWIRRLNPDCKVEVLTPDFKGVYEALSVVIDAKPDV
                     FNHNVETIPRLYKRVRPQAIYTRSLQVLKWAKEMNPAAPTKSGFMLGLGETWDEVIQV
                     MRDLREHEVDILTIGQYLRPSFQHLPIQRYIPLEEFAALKAEGKQMGFRHVESGPFVR
                     SSYHAHEQADGATAQ"
     gene            1755..>2471
                     /locus_tag="VGT44_11430"
     CDS             1755..>2471
                     /locus_tag="VGT44_11430"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2581451.1"
                     /translation="MKAFINNTNLAYTDHGVGLPVIFLHAFPLNRGMWDGELAALLSE
                     RRYRLVSLDWRGFGESEIPNEISTMELFADDVAGLMDQLGIEKAVLCGLSMGGYAAFA
                     FLRSYPERLAGLILADTKPAADTPEAQANRENVAQLAETQGPSAIADLQLPRLLSDYT
                     REHHPEVELRARQMIDAATAQGIAAASRGMARRADASDLLTSVACPTLVIVGEYDAII
                     PPAIAQDYASSIPGAQVVVIP"
BASE COUNT          489 a          778 c          755 g          449 t
ORIGIN      
        1 gagcgccatt cctggcacgg gtaccagcgg gcgcgtgcgc aaggatgaca tcctcgccta
       61 cctcgatcag cgccaggctg ccccggcagc gccgcaagcg ccgtctcccg ctcccgctcg
      121 cgtggcgata gcgcccgccg cgcccgctgc tcccacattc gccggccagg atgaagaact
      181 gatcgagccg agccgcatgc gcctggcgat tgccgaacat atggtgcgca gcaagcacac
      241 gtcgccccat gctacgaccg tcgtcgaggt tgatatgacc aacatcgcga agtggctgga
      301 gcgcaataag gaagacttta agcggcgcga gggctacagc atcagctttg tgccgttcgt
      361 catgaaggcc gtctgtgagg ggctgcgcaa attcccatac atgaacgcga gctggaccga
      421 ggataacaag atcgtgctaa agaagcgcat caacctgggc atggccgtag caacggatgc
      481 cgggctggtg gtgccgacca tctacgacgc cgaccagttc acgctggcgg gcctggcgaa
      541 gcaggtcaac gccgttgcgc agcgcgctcg cgccaacaag ctgaccatcc aggatatgac
      601 gggcagcacc tttgtcgtga ataatcctgg cacatttggt acaattatat cggtaccgat
      661 catcaaccag ccccatgccg gtattctgag catggatgcc gtcgtcaaac gcccggtggt
      721 gatcgaagat gacgccatcg ccgtgcgctc gatgatgtac ctgtgcctct cgttcgatca
      781 tcgcatcaac gatggcgcgg gcgcgggcgg cttcttgcag gctgcgcgcc agaaattgca
      841 gtcctacggg cgcgaaatcg acgtatacta aaggatacta ttatggcgac catgatacca
      901 gagcgccgcc ccgaatggct cagggtgcgc gcccccaggg gtgataacta cgataatctg
      961 aagcagctta tgcgcagcaa gggcctgcac accgtctgcg aagaggcgcg ctgtcccaat
     1021 atcggggagt gctggggcta taaaacggcc accttcatga tcctggggcg cgtctgcacg
     1081 cgcagttgcg ggttttgcgc cgtggagaca ggccgcccga tcgggctgga ctgggaggag
     1141 ccgaaacggg tcgccgaggc ggtgcaggat atgggtctgc gccacgccgt cgtcacctcg
     1201 gtcaatcgcg acgagctgaa ggatggaggc gcggccattt tcgcctcgac cattcgctgg
     1261 attcgccgcc tcaatcccga ttgcaaggtc gaggtgctga cgcccgattt caagggcgtc
     1321 tacgaggcgc tcagcgtcgt catcgacgcg aagccggatg tattcaacca taacgtcgag
     1381 accattccgc gcctctacaa gcgcgtgcgg ccgcaggcca tctacacgcg ctcgctgcaa
     1441 gtcttgaagt gggcgaaaga gatgaacccg gctgcgccga ccaagtcggg cttcatgctg
     1501 ggtctgggcg agacctggga cgaggtgatc caggtcatgc gcgacctgcg cgaacacgag
     1561 gtcgatatcc ttaccattgg ccagtacctg cgtccgtcgt tccagcacct gcccatccag
     1621 cgctacattc cgttggagga gttcgccgcg ctcaaggccg agggcaagca gatgggcttc
     1681 cgccacgtcg aatccggccc gtttgtgcgc agttcgtatc atgcccacga acaggctgat
     1741 ggggcaaccg cgcaatgaaa gccttcatca acaataccaa tctcgcctat accgatcatg
     1801 gtgtggggct gccagtcatc ttcctgcacg ccttcccgct caatcgcggc atgtgggatg
     1861 gcgaactggc cgcgctgctc tcggaacgcc gctatcgcct ggtctcgctg gactggcgcg
     1921 gctttggcga aagtgagatt cccaatgaaa tttcgacgat ggaactcttc gcggatgatg
     1981 tcgctggcct gatggatcag ctcggcatcg aaaaagctgt gttgtgcggc ctctcgatgg
     2041 gtggttacgc agccttcgcc ttcctgcgca gctaccctga gcggctcgcc ggtctaatcc
     2101 tggccgatac aaaacccgct gccgatacgc ccgaagcgca ggctaatcgc gagaacgtgg
     2161 cgcagttggc tgaaacgcaa gggccgagcg ccatcgcgga cctgcaactc ccccgtttgc
     2221 tttcggatta cacccgcgaa catcaccccg aagtagaact gcgagctcgc cagatgatcg
     2281 atgccgccac cgcgcaaggc atcgcggctg cctcacgcgg catggcgcgc cgggccgatg
     2341 cgagtgacct gctgaccagc gtcgcctgcc ccacgctggt aatcgttggc gaatacgacg
     2401 ccatcatccc gcccgccatc gcgcaggact acgcctcgag catcccaggc gcgcaggtgg
     2461 tggtgatccc g
//
LOCUS       DASZAK010000650         2468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_299969, whole genome shotgun sequence.
ACCESSION   DASZAK010000650 DASZAK010000000
VERSION     DASZAK010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299969"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VGT44_11435"
     CDS             <1..986
                     /locus_tag="VGT44_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581452.1"
                     /translation="DILEDIHTLAKHAAAYYKLAVPPQKIESCHDAYLVMRLGESQNL
                     RDAALAYARARLREVNGQVCVPILEQDARRGLYGMQVAQRQETAFAAATLLAGRETTD
                     VPKAIAATNYLTSQLNEEGRLYSTVDTAACLSLLLGLREVGIVSTADGGRVELNRQQM
                     SLADALAFNETVKTLRCIEGVIAAQITSEVIEDWDAFKSAIPVEARLERDGRAQKHFR
                     VGDALELVISVPRYEPGLVAHICLPDALARIAGGGQVKRFSLDFCGNTTLRVPLAAIS
                     ATALPSVASARRWFATAGHLAPTQHWAVIVRNMYKEEQVGNPGRLTVEVNE"
     gene            1282..2148
                     /locus_tag="VGT44_11440"
     CDS             1282..2148
                     /locus_tag="VGT44_11440"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV2581453.1"
                     /translation="MATPDPDASPATQPLDEQHTAPHLTSVLAFNDSPRVTRLLADLR
                     TQTGTLGQFNNSTTSLRQPIIIPGSGKRSQGLTRPPVGRKWVISSAIIAILAVLTVLT
                     GLAVAPLATGNGQIFGGIFSAPGSIHSTSNSLNNQYLAAQAATQTALNHTDGYGGSSS
                     NSGLPVYNSAGVAPTRFAYGNCTYWADQEYYNRTGHLVQWIGNADQWAAGAQAAGWIV
                     SSSPHVYSIIVLQPGVEGAGYYGHVAVVEKINTDGSVVTSNMNWYYGGGWDIISTVVF
                     KPGPGVLFVWHP"
     gene            2201..>2468
                     /locus_tag="VGT44_11445"
     CDS             2201..>2468
                     /locus_tag="VGT44_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581454.1"
                     /translation="MSLRQLQEAREKRFDRLSSMPSPVLNEPSLEEQVYPCTIDDIHY
                     SENAILLFCHNSVIKKLLPYKDKRYDLEDILNRHACLLEGLKWNR"
BASE COUNT          541 a          742 c          693 g          492 t
ORIGIN      
        1 tggacatcct ggaagatatc cacacgttgg cgaaacatgc cgccgcttac tacaagctgg
       61 ccgttccccc acaaaagatc gaaagttgtc acgatgccta cctggtgatg cgcttgggcg
      121 agtcgcagaa cctgcgcgac gcggcgctgg cctatgcgcg cgcgcgactc cgcgaagtca
      181 acggccaggt ctgtgtcccg atattggagc aagatgcccg gcgcggcctg tatgggatgc
      241 aggttgcgca gcgccaggag acagccttcg ctgccgcgac gctgctggct ggccgcgaga
      301 cgaccgatgt gccaaaagcc attgccgcca cgaactacct gacgagccag ttaaacgagg
      361 aagggcggct ctatagcacg gtggacacgg cggcatgtct ctcgctgctg ctgggtctgc
      421 gcgaggtggg catcgtctca actgccgacg gcgggcgagt ggagttgaac agacagcaga
      481 tgtcgctggc tgatgcgctg gccttcaacg aaacagtgaa aacgctgcgt tgtatagaag
      541 gagttattgc agcgcaaata acgtcggagg tcatcgaaga ttgggacgct ttcaagagcg
      601 ccatcccggt cgaggcgcga ctggaacgcg atggacgcgc gcaaaaacat ttccgcgtgg
      661 gtgacgcgct cgaactggta atcagcgtac cacgctacga acctggcctg gtcgcgcata
      721 tctgcctgcc ggacgctctg gcgcgtatcg cgggcggcgg gcaggtcaag cgttttagcc
      781 tcgatttctg cgggaataca acgctgcgcg tccccttggc cgccatcagc gcaaccgctc
      841 tgccgtcagt cgcatcggcg cggcgctggt ttgccacagc cggccacctc gctcccacgc
      901 agcactgggc ggtgattgtg cgcaatatgt acaaagagga acaggttggc aatccagggc
      961 gattgacggt ggaagtcaat gagtaagcaa gtaccatttt cctatggttg accttgcgtt
     1021 gattgactta caaggaaaaa aagctacact ggttaataag ccgcagtatg cgcggtgtgg
     1081 ggcgattcgt cgccccgcgc aaatctgatg gatcggggtt atcgtatgtc gtcgtttgag
     1141 aacggtcagc ccacaaatgc gggcctacag ataccaggat tggttaatca gctttcaccc
     1201 gtagagcagg tgccttttcc tgataccaac acttattttt ttgcctctaa gcggcgtttc
     1261 gactctaccg ggaagcccgc aatggcgacg ccagacccgg atgcctcgcc cgccacgcag
     1321 ccgctcgacg aacagcacac tgcaccgcat ctcacatccg tgctggcttt caatgactcg
     1381 cctcgcgtga cccgtctgct cgccgacctg cgcacccaga ccggcactct gggccagttc
     1441 aataactcaa cgacctcact gcgccaaccg atcatcatac ccggcagtgg caaacgctct
     1501 cagggactca cgcgcccccc tgtaggccgc aaatgggtaa tcagcagcgc cattatcgcc
     1561 atcctggcgg tgctgaccgt gctgaccggc ctggcagtcg cgcccctggc caccggcaac
     1621 ggccagattt tcggcggcat tttctccgcc cccggcagca tccacagcac ttccaactcg
     1681 ctcaacaacc agtacctggc ggcccaggcc gccacgcaaa cggccctcaa ccatacagac
     1741 gggtacggtg gtagttcctc caatagcggg ctgccggtct ataacagcgc tggcgtggct
     1801 cccacccgtt ttgcctatgg caactgcacc tactgggccg accaggaata ctataaccgt
     1861 accggccacc tggtgcaatg gatcggcaac gccgaccaat gggcagcagg agcgcaggcc
     1921 gccggatgga tcgtctcctc cagcccccac gtctactcga tcatcgtcct ccaacctggc
     1981 gtagaaggtg cgggctacta cggccacgtc gccgtcgtcg agaaaatcaa caccgatggc
     2041 agcgtcgtca ccagcaacat gaactggtac tacggcggcg gctgggacat catctcgaca
     2101 gtcgtcttca aaccaggccc cggcgtgttg ttcgtgtggc acccgtaaga gaaaatatta
     2161 gagttaacag ctcgcttgct gttagattgg gaggttcgaa gtgtcgttga ggcagttgca
     2221 agaagcaagg gagaagcgat ttgacagatt atcctcgatg ccatctcccg ttctcaacga
     2281 gccttcactt gaggaacaag tctatccttg tactatcgat gatatccact attctgaaaa
     2341 tgcaatcttg ctcttctgcc acaattctgt cattaaaaaa ctcctgcctt acaaggacaa
     2401 gcgctatgat ctcgaagata tactaaatcg ccatgcgtgt ttgctagagg ggctaaaatg
     2461 gaatagag
//
LOCUS       DASZAK010000651         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_299990, whole genome shotgun sequence.
ACCESSION   DASZAK010000651 DASZAK010000000
VERSION     DASZAK010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299990"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..1626
                     /locus_tag="VGT44_11450"
     CDS             52..1626
                     /locus_tag="VGT44_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2581455.1"
                     /translation="MPMLKRPGTWRRFVAVLVLAALLAACGGSPSVQGTPTPLPCDTS
                     ATTGATSVPPGTPVEDVSSATEWPSPNENLYNTRVAHSTIDSTNVSKLGMAWTIPLQG
                     KGATGADVANPVIANGIAYLQDGASNVIAVQDATGQVLWTRKYNSPDYGPNGVTFANG
                     RIYGVTACGVFALDAQTGQQVWYNSQLATGQVNFDIAPQVANGKVFVSSAITFGGGII
                     YALDAASGTTLWSFQTVVDKVGHQLKATAGGAWDAMLIGPDNSVYAGIGNPYLSLQEA
                     QATPSRELYTDSIVKLNQTTGQLEWYYQAFPNDFHDWDLQISPIYTTVAGRPVVLAAG
                     KGGFVFAFDAKSGQLLWKTAVGIHNGHDDDDQLALDGTLHLQTPYTLEPGEAGGVETN
                     MAAADGMVYVPVDNMPETFATATARVGTSNFFQGKGEMVAIDIATGKPQWATQLPQLL
                     FGGATVSNDLVLTTTFTGELLALSRQDGSVVWTAQLPAGSNATLAIVGNTLLAGAGIP
                     LTAKQHPVVVAYRLGA"
     gene            1679..>1995
                     /locus_tag="VGT44_11455"
     CDS             1679..>1995
                     /locus_tag="VGT44_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2581456.1"
                     /translation="MNAIETNQLTRAFGSLVAVDDLTLAIPEGTVFGFLGPNGAGKTT
                     TVRLLSALIAPTRGSAAVAGHRLGEHNEAIRQAVGILTETPGLYDRLSAWQNLLFFAE
                     MYDL"
BASE COUNT          406 a          592 c          604 g          393 t
ORIGIN      
        1 attgtaatcg ttgtaaaaaa cgatgcgtac tcgtcgctaa aggagttgaa gatgcctatg
       61 ttgaaacgac ccggcacatg gagacgtttt gtggccgtcc tggtactcgc tgccttgctc
      121 gcagcgtgcg gcggctcgcc atcagtgcaa ggcaccccga cgccgttgcc atgcgacacg
      181 tcggctacaa ccggtgctac gtctgtccca cccggcaccc ccgtggagga tgtttcctcc
      241 gcgacggagt ggccctcccc gaatgagaac ctgtacaaca cgcgggtcgc gcactcgacc
      301 attgattcga caaacgtgtc gaagctgggg atggcctgga cgatcccact acaaggcaaa
      361 ggtgcgactg gtgcggacgt tgccaatccg gtgattgcca acggcatcgc ctatcttcag
      421 gatggggctt cgaatgttat cgcggtgcag gatgcgactg gccaggtgct ctggacacgc
      481 aagtacaact ctccggatta tgggccaaac ggcgtgacct tcgccaatgg caggatctac
      541 ggcgtcacag cttgtggggt gttcgccctt gatgcgcaaa caggtcaaca agtgtggtat
      601 aattcgcaac tcgcgacagg ccaggtcaac ttcgacatcg ccccacaggt ggccaacggg
      661 aaggtctttg tctcgtcggc aatcaccttt ggtggcggta tcatctatgc cctcgacgcc
      721 gccagcggca ccacactctg gagcttccag accgtggtgg ataaggtggg tcatcagctc
      781 aaagcgaccg cagggggtgc ctgggatgcc atgctgatcg gcccggataa ctccgtctac
      841 gcgggcatcg gcaatcccta tctctcgctc caggaggcgc aggcgacccc cagtcgcgaa
      901 ttgtacacgg atagtatcgt caagctcaat caaacgacgg gtcaattgga atggtattac
      961 caggcattcc ccaacgactt tcatgattgg gatctacaga tctcgcccat ctataccacg
     1021 gttgccgggc gcccggtggt actcgccgcc ggcaaagggg ggtttgtgtt cgccttcgac
     1081 gccaagagcg gccagttgct gtggaaaaca gcagtcggta tccataacgg acacgacgat
     1141 gatgaccagt tggccctcga cggcacactc catctacaga ccccgtacac gctggaacca
     1201 ggagaagccg gcggggtgga gacgaacatg gcggctgcgg acggcatggt gtatgtgccg
     1261 gttgataaca tgccagaaac gttcgcgact gccacagccc gcgtgggcac gtcaaacttc
     1321 ttccagggca aaggtgagat ggttgccatc gacatagcca cagggaagcc acagtgggca
     1381 acccaactgc cacaattgct gtttggaggg gcgacggtct ccaatgatct cgtgttgacg
     1441 accacgttca ccggcgaact cctggcgctc tcgcgccagg atggatcggt cgtctggacg
     1501 gcgcagctcc cggctggctc gaatgcgacg ttggccatcg ttgggaatac gctgctcgcc
     1561 ggggcaggca tccccctgac ggcgaagcag cacccggtgg tggtcgccta tcgacttggc
     1621 gcgtagggcg tgaggctccc ttgcggcctg cctttttgaa gtagtgaagg atgaaccgat
     1681 gaacgctatc gaaaccaacc aattgacccg tgccttcggc agcctggtgg ctgtggacga
     1741 cttgacgctt gccattccag agggaaccgt ctttgggttc ctgggaccca atggcgcggg
     1801 caaaacgacc accgtgcgcc tgctctcggc cttgattgcc cccacgcgcg gcagcgctgc
     1861 ggtggcggga catcgcctgg gcgagcacaa cgaagccatt cggcaagccg tcggcatcct
     1921 caccgagacg ccgggcttgt atgaccgcct cagcgcctgg caaaacctgc tgttttttgc
     1981 ggagatgtat gatct
//
LOCUS       DASZAK010000652         2453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_16649, whole genome shotgun sequence.
ACCESSION   DASZAK010000652 DASZAK010000000
VERSION     DASZAK010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16649"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1932
                     /locus_tag="VGT44_11460"
     CDS             223..1932
                     /locus_tag="VGT44_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEV2581457.1"
                     /translation="MSTTRSRQQDQASQAEAAAIVPTAGRSTQGWLLVLLVFVAGACS
                     LAVELSASRLLAPYFGTSLFVWANLIGLILLYLTLGYYLGGRLADRFPRPGVLYTLTT
                     IAAFAISLIPFLSRPILSWSQSSFATYSIGVFYGSMASVILLFAAPLILLGCVSPFAI
                     RLRIDQLGRSGRTAGQLYAISTAGSILGTFLPVLWLIPSIGTYRTFFTIAVALLLVSV
                     AGLVVSRPANTASGPPDPSDLSNPSNPPNATKRKRFNKNTLSILLLIPMALSVFSIRG
                     PIKPAFGSNGGGILLAERESAYNYIQIVQVGDETQLILNEGVGIHSVYNPHAILTGGY
                     WDYFLVAPYFNNPPYTPEQFHKVAIIGLGAGTAARELTAAYGSIPIDGVEIDGTIIDL
                     ARQYFDMNEHNLNVIVQDGRYWLDTSSQKYDEIAIDTYQQPYVPFQFTTREFYQAVRD
                     HLTPTGVVAVNAGRTATDFRLVEALAQTMHSVFSNVYIIDTQRFYNSVIIGTNAPTSL
                     SNFAVNTASLTNPYLAQVANASITYGNMREERNSTVWFTDDRAPVEQLIDQIIFDDVV
                     NQR"
     gene            1966..>2453
                     /locus_tag="VGT44_11465"
     CDS             1966..>2453
                     /locus_tag="VGT44_11465"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV2581458.1"
                     /translation="MLDLSNMRVIDLSQNWDVNTPSFATYEGPTVKWIKRPAFEKVGG
                     QFISSTLHVGTHLDAPLHFVTNGQDIGSIPLSKLVGPGVVVDLEAMGLGDYDIYTSQH
                     FEEWERKTGIRIEPGDIVVVHTGYHKYYQSNWYGVCEPNEIRYFNMHPGPTREFVDWT
                     LKR"
BASE COUNT          477 a          827 c          627 g          522 t
ORIGIN      
        1 atgtacccgc tgaggtctgc tcgatctgcg gcgatgtgct gttcacgcca gagactgtac
       61 gccggatcga ggctatccgg cgcactaccg ctccaccggc acgtactgta ccgctttatg
      121 agttctcctc ggcaaaatca gcataaccgc gctgcatttt cgtctttttt tgtgcttccc
      181 accatctctc aaacttctca ggcgtagaaa gcgagtttcc gtatgtccac cactcgttct
      241 cgtcaacagg atcaggcgag ccaggcagaa gctgcggcca ttgtcccgac ggcggggcgc
      301 agcacgcagg gctggctgct cgtcctgtta gtcttcgtgg cgggggcgtg ttcgctggcc
      361 gtcgagctat cggcctcgcg gctgctggcc ccctatttcg gcacctcgct cttcgtctgg
      421 gcaaatctga tcggactgat cttgctctat ctcacgctcg gctactacct gggcggacgc
      481 ctggccgacc gcttcccgcg tcccggcgta ctctataccc tgaccaccat cgccgcgttt
      541 gccatcagcc tgatcccttt cctctcccgg cccatcttga gctggtcgca gagttcgttt
      601 gccacctatt ccatcggcgt cttctacggc tcgatggcct cggtgatcct gctcttcgcc
      661 gcgcccttga tcctgctggg ctgcgtctcg cccttcgcca tccgcttgcg catcgaccag
      721 ctcggcaggt cggggcgcac cgccggtcag ctctacgcca tttcgacggc gggcagcatc
      781 ctgggaacct tcctgcccgt cctctggctg atcccctcga taggcaccta ccgtactttc
      841 ttcaccatcg ccgtcgcgct gctgctcgtc tcggtcgccg gcctggtcgt atcacgaccc
      901 gccaacactg catccggccc gccagaccca tccgacctat cgaacccatc aaatccgccg
      961 aacgcgacaa agcgaaagcg tttcaacaaa aataccctat caatcctcct gctcatcccg
     1021 atggcgctct ccgtcttctc cattcgcggc cccatcaaac cggcctttgg cagcaatggc
     1081 ggcggcatcc tgctcgccga acgcgaatcg gcctataact acatccagat cgtgcaggtc
     1141 ggcgatgaga cgcagttgat cttgaacgaa ggggtgggca tccattccgt ctataacccc
     1201 cacgccatcc tcaccggcgg ctactgggat tattttctgg tcgcgcccta tttcaacaat
     1261 cctccctaca cgccggagca gtttcacaaa gtcgccatca tcggcctggg cgcgggaacg
     1321 gctgcgcgcg agttgaccgc cgcctacggc tccattccca tcgacggcgt cgagatcgac
     1381 ggcactatca tcgacctggc gcgccagtac tttgacatga acgagcacaa cctgaacgtc
     1441 atcgtccagg atggacgcta ctggctggat acgtccagcc agaagtatga cgaaatcgcc
     1501 atcgacacct accagcaacc ctacgtgcca ttccagttca ccacgcgcga gttttaccag
     1561 gcggtgcgtg accacctgac gccgacaggc gtggttgccg tcaatgccgg ccgcaccgcg
     1621 accgatttcc gcctcgtcga agcgctggcg cagaccatgc actccgtctt ctccaacgtc
     1681 tacatcatcg atacacagcg cttctacaac agcgtcatca tcggcaccaa cgcgcctaca
     1741 tcgctcagca actttgccgt caataccgcc tcgctgacaa acccatacct ggcgcaggtc
     1801 gcgaacgcca gcatcacgta tggcaacatg cgcgaggaac gcaacagcac cgtctggttc
     1861 accgatgatc gcgcccccgt cgaacaactc atcgaccaga tcatcttcga tgacgtggtc
     1921 aatcaacgat aatgtccgtg taatttgcca tgaaggagtg agccaatgtt agacctgagc
     1981 aacatgcgcg tcattgatct gtcgcagaac tgggatgtca acactccctc gttcgcgacc
     2041 tacgaggggc caacggtcaa gtggatcaag cgacccgctt ttgaaaaagt aggcggccag
     2101 tttatttcgt ccacgctgca cgtcggcacc cacctggatg cccccctgca cttcgtcacc
     2161 aacggccagg atatcggcag cattccactc agcaagctgg tcggtcctgg tgtggtggtg
     2221 gacctggagg cgatgggact cggtgattat gatatttata cctcgcagca tttcgaggag
     2281 tgggagcgca agaccggcat ccgcatcgaa ccaggcgata tcgtcgtggt gcataccggc
     2341 taccacaagt attaccagag caactggtat ggcgtgtgcg aaccaaacga gattcgctac
     2401 ttcaacatgc atcccggccc gacgcgcgaa ttcgtggatt ggacgctgaa acg
//
LOCUS       DASZAK010000653         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_548505, whole genome shotgun sequence.
ACCESSION   DASZAK010000653 DASZAK010000000
VERSION     DASZAK010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548505"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..600
                     /locus_tag="VGT44_11470"
     CDS             <1..600
                     /locus_tag="VGT44_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2581459.1"
                     /translation="GFYRDPSGKEFTMSVDVVNGWSDWDQDVQFIVNDLKAAGINATL
                     NSESGFTPYYTAITTGAYDAAISWTDSGPTPYYPFHDMLLSANAGNPSANKAAVGSNF
                     EGWKTSTSNGLAAQTDRLLAQYKASPDGSPAQMQAIQGIEDIMVNDLPAIPLTVNVTW
                     MEYRTANWVGWPDATNPYDIGPPYQMPDAANVILHLKPA"
     gene            686..1678
                     /locus_tag="VGT44_11475"
     CDS             686..1678
                     /locus_tag="VGT44_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2581460.1"
                     /translation="MRYFLRRIGFYLIALWAAVTFNFIIPRLMPGDPAEAYIAKIQAQ
                     NVTRSQLAAIRAEFGVNPNVPIWKQYFDYLNALLHGQLGIATSRFPSQVSDILGQSLP
                     WTIGLVGTAVFLSFFIGTLVGMFIAWRRGSLFDSTIPPLLTFFSAVPYFWMALALIYI
                     FAVNLGWFPTGNGYDVFGATPSWTPDFISSVVQHAFLPVITLVIGSLAQWVLTMRNTM
                     VTTLSEDYILMAEAKGLPQRRVMFNYAARNAILPSITSFSISLGLVVSGSLLTEIVFN
                     YPGIGFALYNGVQGDDYALVEGCFLVIALAVLIANFFADLVYVFLDPRVRQGRG"
BASE COUNT          354 a          479 c          464 g          428 t
ORIGIN      
        1 ggattctatc gcgaccccag tggaaaagag ttcacgatgt cggtagacgt tgtcaatggc
       61 tggagcgact gggatcagga tgtccagttt attgtgaatg acctcaaggc cgccggtatc
      121 aatgcgacgc tcaattcgga gagcggcttc acaccgtact acaccgccat tacaaccggc
      181 gcgtatgatg ctgcgatcag ctggacggat agcggcccta ctccgtacta tccgttccac
      241 gatatgctgc tcagcgcaaa cgcgggtaat ccttccgcga acaaggcagc tgtcggctcg
      301 aacttcgagg gctggaagac ttcaaccagc aacgggcttg ctgctcagac cgataggctg
      361 ctcgcgcagt acaaggcgag tcctgatggc tcccctgcgc agatgcaggc tattcaaggc
      421 attgaggata tcatggtgaa tgatttgccg gctattccct taaccgtcaa cgtcacctgg
      481 atggaatacc ggacggccaa ctgggttggt tggcctgacg caaccaatcc ctacgatatt
      541 ggccctccgt accagatgcc tgacgcggcg aacgtcatcc tccacctgaa gccagcctag
      601 tcgtagcgcg tccttctcat gtgtccttcc atctcccaat catggggagg gggatggaag
      661 gacaggaccc cctaaggaga gagtaatgcg ctactttttg cggcgtatag gcttctacct
      721 gatagcgctc tgggctgcgg tcacgttcaa tttcatcatc ccgcgcttaa tgccgggtga
      781 tccagccgag gcatatattg caaaaattca ggcacaaaat gtcacccggt cgcagcttgc
      841 cgccatccgg gccgaatttg gggtgaaccc gaatgtacct atctggaagc aatacttcga
      901 ttatctaaac gctttactac atggtcagct gggcatcgcg acctcgcgtt ttccctcaca
      961 ggtctcagat attctggggc agagtttacc ctggacaatc ggcctggtcg gaacagctgt
     1021 cttcctgagc tttttcatcg gtacgctggt aggtatgttt atcgcctgga ggcgcggctc
     1081 cttgttcgat agcactatac ccccgctcct gacctttttc tccgccgttc cttatttctg
     1141 gatggcgctg gcgctgattt acatttttgc cgtgaacctg ggctggttcc ccaccggtaa
     1201 tggctatgat gtctttggcg ccactcccag ctggacgccg gattttatca gcagcgtcgt
     1261 tcagcatgcc ttcctgccgg ttattacgct tgtgattggc tcgctggcac aatgggtgct
     1321 cacgatgcgc aatacgatgg tcacgacgct ttcggaggac tatattctga tggcggaggc
     1381 caaaggtctg ccacagagac gggtgatgtt taattacgcg gcgcgcaacg ctattttgcc
     1441 cagcatcacc agcttctcga tctcactggg cctggttgtc agcggctcgt tgctcacaga
     1501 gatcgtcttc aactaccctg gcatcggctt cgctctgtac aatggcgttc aaggcgatga
     1561 ttacgccctg gtggaggggt gtttcctggt aatcgcgctc gccgtactca tcgcgaattt
     1621 cttcgcggac ctggtctatg tgttccttga tccacgtgtg cgacagggaa ggggctaagc
     1681 gatgcaagtc acgaagatag atcaacatat tgaaacctca actat
//
LOCUS       DASZAK010000654         2284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_621803, whole genome shotgun sequence.
ACCESSION   DASZAK010000654 DASZAK010000000
VERSION     DASZAK010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621803"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..563)
                     /locus_tag="VGT44_11480"
     CDS             complement(3..563)
                     /locus_tag="VGT44_11480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEV2581461.1"
                     /translation="MTTTPDDARPAAPPLIRLATPQDLPAIEDLDRLSASPTRDIHRD
                     VEKYFGSVDPSTHERTLIFLVQVGEFIAGKAELMTPPPQPEGAPTNQDILTRASVGYV
                     KRVVVDPRFRKLGLARAMMLHIIAYAQSDLRLSAIDLHVWEDNQPAIRLYESLGFHLQ
                     HRELYFRLPLYEIKFSKGDHKGTPLP"
     gene            complement(576..770)
                     /locus_tag="VGT44_11485"
     CDS             complement(576..770)
                     /locus_tag="VGT44_11485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581462.1"
                     /translation="MNKKVMSTLMLFLVFIGFLWWGLAQILHPDPTDTGESIVFGVLL
                     LGCAGYAFVSILMLPRRRQR"
     gene            complement(767..>2284)
                     /gene="recN"
                     /locus_tag="VGT44_11490"
     CDS             complement(767..>2284)
                     /gene="recN"
                     /locus_tag="VGT44_11490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV2581463.1"
                     /translation="LILSRDIFRSGRTVARINTRTVSQQVLQQIASWLVDIHGQSEHM
                     SLLRPEQHINFLDRYADLLPLRSQLADKVGAWRGDRKTLAELQKSEKELQRRADYLRF
                     EIEEIDKAELRPGEVEEMEAERKILNNAERLRELCVSIYGAISGGEGAGDDFQPALDL
                     LRPAMKAMNELARLDPDLQDQESAFSEAIYQLEDVASSLSRYESDIEDDPQRLAVIEE
                     RLDLISRLKRKYGATIEEILKRADDARAELDTIVHRDELMAELQQRDQVHRREIGQLA
                     AELSARRRRAAGRLSSAMEEQLNDLNMQRARFSVEILQTSDEHGVPASLDGQAEQYYA
                     CDTTGIDRVQFLIAPNPGEPFKPLTKIASGGETSRLMLALKTILSAADATPTLIFDEI
                     DAGISGKSGQIVGQKLWQLTRNHQVVCVTHLPQIASFADTHYNVNKQIFDERTVTLVR
                     ELNPEQRAREISHIMGGFDSNHTLKSAEEMLAYAYRWKEDWTRAAHGEGKEAHNL"
BASE COUNT          419 a          647 c          730 g          488 t
ORIGIN      
        1 agtcatggta agggcgtacc cttgtggtcg cccttcgaga acttaatctc atagagtggg
       61 aggcgaaaat acaattcgcg gtgttgcagg tggaagccga gcgactcgta gaggcgaatg
      121 gcgggctggt tgtcttccca gacatgcagg tcgatggcgc tcaggcgcag gtcggattgg
      181 gcataggcta tgatatgcag catcatggcg cgcgccaggc ccaacttgcg gaagcgcggg
      241 tcgaccacga cgcgcttgac atagccaacg ctcgctctgg ttagtatatc ctggttggtc
      301 ggcgcgcctt cgggttgcgg cgggggtgtc atcaattctg ctttgcccgc gataaattct
      361 cccacctgga caaggaaaat cagcgtgcgc tcgtgtgtgg aagggtcaac cgagccgaaa
      421 tatttctcca cgtcgcgatg aatgtcgcgc gtgggagaag cgctgagccg gtcgaggtcc
      481 tcgatagccg ggaggtcctg cggcgtggca agacgaatga gcggcggcgc agccgggcgg
      541 gcatcatcgg gtgtggtggt catggctgcg ccgcatcagc gctgccgcct gcgaggcagc
      601 atcaggatcg agacgaaggc ataaccggcg cagccgagca gcagcacgcc gaaaacaatg
      661 ctctcgcctg tatcggtcgg atcggggtga agaatctgcg ccagccccca ccagagaaag
      721 ccaatgaaaa cgagaaagag catcagggtg ctcatgactt ttttattcat agattgtgcg
      781 cttcctttcc ctcgccatgc gcggcgcgcg tccagtcctc tttccagcga tacgcataag
      841 caagcatttc ctccgcgctt ttcagcgtgt ggttgctatc gaagccgccc ataatatgcg
      901 agatttcgcg cgcgcgctgt tcaggattga gttcgcgaac gagcgttacg gtccgttcgt
      961 caaagatctg tttattcaca ttgtaatgcg tatcggcaaa tgaggcaatt tgcggcaggt
     1021 gcgttacgca gacgacctgg tgattgcgcg tgagctgcca gagcttttgg ccgacaatct
     1081 gcccgctttt gccgctgatg cccgcatcga tttcatcgaa gatgagcgtc ggggtggcat
     1141 cggctgccga cagaatcgtc ttgagcgcca gcatcaggcg cgaggtctcg ccgcccgatg
     1201 cgatcttcgt caggggcttg aacggctcgc cggggttggg cgcgatcaag aattgtacgc
     1261 ggtcgatgcc ggtggtgtcg caggcgtaat actgctccgc ttgcccatcc aggctggccg
     1321 gaacgccatg ttcgtcagat gtctggagta tttcgacgga gaagcgcgcg cgctgcatat
     1381 tcaggtcgtt caactgttct tccatagcag aggaaaggcg gccagccgct cggcggcggc
     1441 gcgcggagag ttcggcggct agctggccaa tctcgcgccg gtgaacctgg tcgcgctgct
     1501 gcaactcggc catcagttca tcgcgatgca cgatggtgtc gagttccgcg cgggcatcgt
     1561 cggcgcgttt gagaatctcc tcgatggtcg cgccatattt acgtttcagg cgcgagatca
     1621 ggtccaggcg ttcttcgata accgccaggc gctgcgggtc gtcctcgatg tcgctttcgt
     1681 agcgactgag agacgaagca acatcctcaa gctggtaaat ggcctccgag aaagcgcttt
     1741 cctgatcttg taagtctgga tcgaggcgcg ccagctcgtt catggctttc atggcggggc
     1801 gcagcaggtc cagggcaggc tgaaaatcat cgcccgcgcc ctctccgccg ctgatcgctc
     1861 catagatgga cacgcatagc tcgcgcaggc gttcggcatt gttgagtatc ttacgctcgg
     1921 cttccatctc ctcgacttcg ccgggccgca actcggcctt gtcgatctcc tcgatctcga
     1981 agcgcaggta gtcggcgcgg cgctgcagct ccttctcgct tttttgcaac tcggccagcg
     2041 ttttgcggtc cccgcgccag gcgcctacct tgtcggctag ctggctgcgc aggggcagca
     2101 ggtcggcgta acggtccagg aagttgatgt gctgttcggg acgcagcagg ctcatatgct
     2161 cgctctggcc gtgaatatcg accagccagc tcgcgatttg ctggaggacc tgctgcgaga
     2221 cggtgcgcgt gttgatgcgc gccaccgtgc ggcccgaacg gaagatgtcg cgcgagagga
     2281 tcaa
//
LOCUS       DASZAK010000655         6090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_51270, whole genome shotgun sequence.
ACCESSION   DASZAK010000655 DASZAK010000000
VERSION     DASZAK010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51270"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..772
                     /locus_tag="VGT44_11495"
     CDS             95..772
                     /locus_tag="VGT44_11495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEV2581464.1"
                     /translation="MSTRQAQDTFLPRRGKRVSSVKSPHVARSTVADTWRWLWPDTLM
                     RILPLGAVPFIYTVIFHLPLAFLGLTLNDWPHQVAIGLLVGIPMTAFAALYRMLIVGP
                     WFRWPTLSDHALQGFFYLFVNGPVEELFFRGFVLAAVTQWTGWIFWGWLVSTTAYTLY
                     HRLGKWSWRSVGGVGLAGIVFSIVYLAQPTPRSLLSVVIVHGLTTWGFLSLGDEVMYR
                     VWKRRQS"
     gene            complement(997..1146)
                     /locus_tag="VGT44_11500"
     CDS             complement(997..1146)
                     /locus_tag="VGT44_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581465.1"
                     /translation="MALILTRADIQRCLSMREAIDAMRVAFQALSAGHAQAPQRGNVT
                     LAEQG"
     gene            1331..2281
                     /locus_tag="VGT44_11505"
     CDS             1331..2281
                     /locus_tag="VGT44_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEV2581466.1"
                     /translation="MKILVTGGAGFIGSHLCARLLEEGHYVQCVDNLLTGSERNIEAI
                     RNHPRFSFLQHDVTIPFQFEADAIYHMASPASPVGYMEHPIETILVNSHATYLMLEQA
                     RKQKAMFLYASTSEAYGDPLVHPQREDYWGNVNPIGPRACYDESKRLGETLTMEYYRQ
                     YQVNARIVRIFNTYGPNSQTDDGRMIPNFITQALQNKPLTIYGDGSQTRSICYVSDLV
                     DGLMRALFTPHTAGEVFNLGNTDERTVLEYAQLIIALCNASSQLIFEPGRIDDPERRR
                     PDISKAQRALGWQLHVSKEEGLRRTIDWFSKQLPHLTTIS"
     gene            2309..3988
                     /locus_tag="VGT44_11510"
     CDS             2309..3988
                     /locus_tag="VGT44_11510"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV2581467.1"
                     /translation="MQQAVASGIKPARRDFWPNLRTVQDFFTIPNVIVALGCFALVFM
                     LIVLPLPWNLRLPLYLAALVWTILRPRAALYLMAFAVPWGSLDYINVSTLRLDSADIL
                     VVFLVIGWLLQWCLPARVRQQQRDEARVPGYLALAMLALLFTMLLSTVVAINIKDSLK
                     EISKWLEVLALLMLGSQYLQTRRQIWTMIVLICLAGITQAIFGYFQAGLNLGPQSFIR
                     GASLRVYGTFDQPNPYAAYINMPLSIALALTLLSRDWLTRLLAGITVVSLGLAELLSQ
                     SRGGEIALAAALTFILLAGMPRVRTLMRIGIFAILAGLAAAMLGLLPSSLTSSALKFL
                     GLEQISFSAPSAQDYSTAERLAHWIAGLNMFFDHPILGVGIGNYPDAYAPYHITIFLD
                     SLGHAHNYYINIAAEMGTIGLVVYLGFVAALFVLGGHAIRSLSRRYHQERSRVQAQPG
                     GKRPIIQKPLELKDKVALLLQPQHMMRVYRSASPLARGRLLANDRALAIGITAALVTV
                     AVHNLVDDVYVHSLTNLIALLLIALIRLERVTPGTETQAAYGGQVDFTESR"
     gene            3966..5108
                     /locus_tag="VGT44_11515"
     CDS             3966..5108
                     /locus_tag="VGT44_11515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV2581468.1"
                     /translation="MISQNPDSVAQETDLNETDSSEIENKKVVHLQPEPEISQSQLSI
                     GKRLLSWRTIIPLVLVIGLLVYFARQANINPQETWAALSSANVLFLLAAFLTYYLSFP
                     IRTLRWRMLLHNIGYTKSNGVKLPRFWKLLEILYISWFANVIVPAKLGDLYRAYLLRK
                     ETDLPTSRTFGTVLAERLLDLIVLLLLFIPAVIISLHENLPVQLQTGLKVTLAAVVLG
                     IVGLFMLRLFPQQIARLIPARFRGHYNHFQEGTLGSFRHVPTLSVLTVFVWSCESFRF
                     LFVALAFNLIGGDPLHIITAALFIALGEALLTVVPFTSGGIGLVEGGMLAMIALFTAK
                     TNVAAAAILADRSISLFSILLIGFIVFMFAFGRDARKAAKQAKNLS"
     gene            5122..5721
                     /gene="ybeY"
                     /locus_tag="VGT44_11520"
     CDS             5122..5721
                     /gene="ybeY"
                     /locus_tag="VGT44_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV2581469.1"
                     /translation="MAIFIDCSINLDEQDIALQRMFDADLPELVSDRTLRVAGIDATA
                     RSEQSYSFSLVITGDDEMQALNRQYRNQDKPTDVLSFPLLDDPLVNAPADQLWMLPED
                     AVADDEGEDQAGTQSPPHPAFITPAELAINLGDIVISWSTVSRQAAQAGHQPVYELLY
                     LLAHGILHLVGYDDQTEAGYAAMVGIQEAVMREIDLGTA"
     gene            5729..>6090
                     /locus_tag="VGT44_11525"
     CDS             5729..>6090
                     /locus_tag="VGT44_11525"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV2581470.1"
                     /translation="MSTQPPPPAQRSAGAKFIAGFGYAFSGLWYALRTQINMWVHLTL
                     ALLAVALGIVLHISAVEFAMIFVAIAGVLIAELFNTVIELCVDLASPGFHPLAKRAKD
                     VAAGAVLLSAMLAIVIAL"
BASE COUNT         1205 a         1773 c         1712 g         1400 t
ORIGIN      
        1 tcgttttgcc tggacaatca gactctaata atttacactt acttaacagt ggcttcaccg
       61 gccgttaaca tactggaacg taagtgagaa gcgcatgtca acacgccagg cccaggatac
      121 gtttttaccg agaagaggaa agcgagtgtc ttccgtcaaa tccccgcatg ttgcccggtc
      181 taccgttgct gatacctggc gctggctttg gccggacacg ctgatgcgta ttctgcccct
      241 gggagctgtt ccgttcatct acactgtgat ctttcacctg cccctggcct tcctcggatt
      301 gacgctgaat gactggccac atcaggttgc gattggtcta ctggtgggca tcccaatgac
      361 cgcgtttgcc gcgctctatc gcatgttgat cgtcggcccc tggtttcgct ggcccacgtt
      421 gagcgaccac gcgctgcaag gattcttcta cctgtttgtc aacggaccgg tcgaggaact
      481 cttctttcgc ggattcgtgc tggcggcggt gacgcagtgg acgggctgga tattctgggg
      541 ctggctggtc agcacgacgg cgtacacgct ctatcaccgc cttggcaagt ggagctggcg
      601 cagcgtgggc ggcgtcgggc tggccggcat cgtcttcagt atcgtctacc tggcgcagcc
      661 cacgccgcga tcgttgctct cggtcgtgat cgtgcatggt ctgacgacgt ggggcttcct
      721 gagcctgggc gacgaggtga tgtaccgggt gtggaaacgg cggcaaagtt agctcacacc
      781 tcgacctcga tgccgatgcc acgctcacac gcccggccat agacatgcag cgcggcgggc
      841 aggacgcgcc gagcagcgtc tccacgaggc gctggtaatg ctcgtcactg cgattgaaga
      901 cgcgcacctc gcgcaggggg cgcggtatga ccgtgacaac ttttaggcca aaggcggccg
      961 gtgtcgtcgt ttgtaagagt gacggtatca gcaatgctac ccctgctcgg cgagtgtgac
     1021 attgccgcgc tgcggggcct gggcatgacc agcgctcagc gcctggaatg cgacgcgcat
     1081 agcatcgatg gcttcgcgca tggacaggca gcgctggata tcggcgcgcg taaggatcag
     1141 tgccatatcg agacaaacct ttcatgatag tcgtaatata gtaatgaggt acaaaatgct
     1201 gtttgggtga cgtttcctcc catattcgtt accttcatag aggcgaatcg tctggcatta
     1261 aagtatgata ctgcatctgg caagatgata tcactacatc tatttctgca tgagttggag
     1321 gagtaatttt ttgaagattc ttgtgaccgg tggggccggg tttattggct ctcatctctg
     1381 cgctcgcttg ttggaggagg ggcattacgt ccagtgtgtc gataatcttc tgaccggttc
     1441 ggaacgcaat atcgaagcga tacgtaatca tccgcgcttt tcttttctac agcatgatgt
     1501 caccatccca tttcaatttg aggccgatgc tatctatcac atggccagtc cggccagccc
     1561 tgttggttat atggagcatc ctatcgagac catcctggtc aatagccacg cgacatacct
     1621 gatgctggaa caggcgcgca aacaaaaggc catgttcctg tatgcctcca catccgaggc
     1681 atacggcgat ccgctggtgc atccgcagcg cgaggactac tggggtaacg ttaaccccat
     1741 cgggccgcgc gcctgctacg atgaaagcaa gcgcctggga gagactctga cgatggaata
     1801 ctatcgtcaa taccaggtca acgcgcgcat cgtgcgtatc ttcaacacct atgggccgaa
     1861 cagccagacc gatgacgggc gtatgattcc gaacttcatc acgcaggcgc tgcaaaataa
     1921 accgctgacc atctacggcg acggcagtca gacgcgcagt atttgctatg tgagcgacct
     1981 ggtggatggg ctgatgcgcg ctctgtttac gccgcatacg gcgggcgagg tattcaatct
     2041 tggcaatact gatgagcgta ccgtgcttga atacgcgcaa ttgattatcg cgttatgtaa
     2101 cgcctcgtcg caactgattt ttgagccggg ccgcatcgat gacccggagc gacgacgccc
     2161 cgatatcagc aaagcgcagc gcgcgctggg ctggcagtta cacgtaagca aggaggaagg
     2221 gctgcgccgc accatcgact ggttcagcaa gcagcttcct cacctgacga ccatttccta
     2281 actagagcat gagaagtagc caatgcgtat gcaacaagca gtcgcgagtg ggataaagcc
     2341 tgcgcgccgc gatttctggc cgaacctacg aacggttcag gatttcttca ccatcccaaa
     2401 tgtgatcgtc gcgcttggct gcttcgcgct ggtgtttatg ctgattgtgc tgccgctgcc
     2461 ctggaatctg cgcctgccac tgtacctggc ggcgctggtc tggacgattc tgaggccgcg
     2521 cgctgcgctc tatctcatgg ccttcgccgt tccctgggga tcgctggact acatcaatgt
     2581 gagtacgttg cgcctggata gcgcggatat cctggtcgta tttctcgtga tcggctggct
     2641 gctccagtgg tgcctgcccg cgcgggtgag acaacagcag cgcgatgaag ctcgcgtccc
     2701 cggttacctg gcactggcaa tgttggcgct gcttttcacg atgctgctct caacggtggt
     2761 cgcgatcaac atcaaagata gcctgaagga gatcagcaaa tggcttgagg tgctggctct
     2821 gctgatgctg ggcagccagt acctgcaaac gcgccgccag atatggacga tgatcgtctt
     2881 gatctgcctg gcgggtatca cccaggccat ctttggctat ttccaggcgg gactcaatct
     2941 tggcccgcaa tcgtttattc gcggcgcttc actgcgcgtc tacggcacct tcgatcagcc
     3001 aaatccctac gctgcctaca ttaacatgcc gctgagcatc gctcttgcgc tgacgctgct
     3061 ctcgcgcgac tggctgacgc gccttctggc gggcatcacg gtcgtgtcgc tcggactggc
     3121 agagctgctc tcgcaatcgc gcggcggtga aattgcccta gcggcagcgc tgacgttcat
     3181 cctacttgca gggatgccgc gcgtgcgcac actgatgcgc ataggcattt tcgccatact
     3241 tgccggactc gcggcggcga tgctcggttt actgccatcg tcactgacga gttccgcgct
     3301 caaattcctg ggattggagc aaatttcctt cagcgcgccg agcgcacagg attattcgac
     3361 tgccgagcgc ctcgcccact ggatcgcggg gctgaatatg ttcttcgatc atcccattct
     3421 gggcgtgggc atcggcaact acccggatgc ctacgcgccc taccatatca ccatattcct
     3481 cgattcactg ggacacgcgc acaattatta catcaatatc gccgccgaga tgggtacgat
     3541 tggcctcgtc gtttacctgg gctttgtagc ggcgctcttc gtcctcggcg ggcacgccat
     3601 tcgctcactc agcaggcgat atcaccagga acgctcgcgg gtacaggcgc agccgggtgg
     3661 gaaacgcccg atcattcaaa agccccttga actcaaggat aaagtggcgc tgctgctgca
     3721 accacagcac atgatgcgcg tctatcgcag cgcatcgccc ctggcgcgcg gacgcttgct
     3781 cgccaatgac cgggcgctgg ccatcggcat caccgcagcg ctcgtcaccg tcgcggttca
     3841 caatctcgtt gatgatgtct atgtccacag tttgaccaac ctgatcgcgt tactcttgat
     3901 tgcgctaatc cgtctagaga gagtaacgcc tggtacagaa acacaggcag cgtatggagg
     3961 acaagttgat ttcacagaat ccagatagtg tggctcagga aactgacctt aacgaaacag
     4021 atagttcaga gatagaaaac aagaaagtcg tacatctcca accggagccg gaaatctccc
     4081 agagccagct ctccatcggg aagcgactgc taagctggcg aaccatcatc ccccttgtgc
     4141 tcgtaatagg gctgctggtg tatttcgcaa gacaggccaa tatcaatccc caggagacct
     4201 gggctgctct tagcagcgcg aatgtactct tcttgctggc ggcatttctg acctactacc
     4261 tttcattccc tattcgcaca ttgcgctggc gaatgcttct tcataatata ggctatacga
     4321 aatccaatgg tgtaaaactg cctagattct ggaaattgct agaaattctc tatatctcct
     4381 ggttcgccaa tgtgatcgtg cctgccaaac tgggtgatct ctatcgcgcc tacttactgc
     4441 gcaaagagac cgacttgccg acttcacgca cgttcggaac cgtgctggct gaacgcttgc
     4501 tcgacctgat cgtattgctg ttgctcttca ttccggcggt catcatcagc ctgcatgaaa
     4561 acctgcccgt tcaactgcaa accggcctga aggtgacgct ggctgccgtc gtactaggca
     4621 tcgttggtct gttcatgctg cgtctcttcc cacagcagat cgcgcggctc atcccggccc
     4681 gtttccgtgg tcactacaac catttccagg aagggacgct cggctcgttc cggcatgtgc
     4741 cgacactcag cgtgctgacc gtttttgtgt ggtcatgcga atcgttccgc ttcttgttcg
     4801 tggcgctggc cttcaatctg atcggcggtg acccgctgca catcattacc gccgcgctgt
     4861 tcatcgcgct cggcgaggcg cttctgacgg tcgtgccttt caccagtggc gggattggcc
     4921 tggtggaggg ggggatgctg gcgatgatcg cgcttttcac tgcgaagact aacgtcgcgg
     4981 cggcggccat cctggcagac cgctcgatca gcctgttcag tattctgctt atcggtttca
     5041 tcgtctttat gtttgcattc gggcgggacg ctcgcaaggc ggcaaaacag gccaaaaacc
     5101 tctcatagcg ctatgcaaga aatggctatc ttcatcgatt gctctatcaa cctggacgag
     5161 caggatatcg cgctccagcg catgtttgac gctgatttgc cagagctggt gtctgatcgc
     5221 acgctgcgcg ttgccgggat cgatgcgact gcacggtctg aacagtcgta cagtttttcg
     5281 ctggtcatca ccggtgatga tgagatgcag gcgctgaacc ggcagtatcg caaccaggat
     5341 aaaccgaccg atgtgctttc ctttcccttg ctcgacgatc ccctggtgaa cgcgccagcc
     5401 gaccaactct ggatgctgcc cgaagacgcg gttgcggatg acgagggaga ggatcaagcc
     5461 ggtacgcaat ctccccccca cccggcgttc atcacgcctg ctgaactggc cattaacctg
     5521 ggcgatatcg tgatttcgtg gtccaccgtc tcgcggcagg cagcacaggc aggccatcaa
     5581 ccggtctacg aactgctcta cctgctggcg catggcatcc tgcacctggt gggttacgac
     5641 gatcagacag aagcgggata cgcggcaatg gttggcattc aggaagcagt catgcgcgag
     5701 atcgacctgg gaacagcttg agaacgccat gagtacacaa ccacctccac cggcgcagcg
     5761 cagcgccggg gcaaagttca tcgcgggctt cggctatgcc ttcagcggtc tgtggtacgc
     5821 gctgcgcacg caaatcaaca tgtgggtaca cctcacgctg gcactgctgg ctgttgcgct
     5881 gggcatcgtc ctccacatca gcgccgttga gttcgccatg attttcgtcg ccatcgcggg
     5941 ggtactcatc gcggaactct tcaacacggt catcgagctg tgcgttgacc tggcctcgcc
     6001 cggctttcat cccctggcga aacgcgctaa ggatgtggcc gcaggcgcag tactgctcag
     6061 cgccatgctc gccatcgtga ttgccctgtt
//
LOCUS       DASZAK010000656         2605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_372749, whole genome shotgun sequence.
ACCESSION   DASZAK010000656 DASZAK010000000
VERSION     DASZAK010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372749"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VGT44_11530"
     CDS             complement(<1..948)
                     /locus_tag="VGT44_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEV2581471.1"
                     /translation="MPEFDVYRVVRRRGEPDVRSLDAYRRAGGYAAVEKALRQQTPEQ
                     LIQQALDAKLRGRGGAGRLTGEKWRIARRQEDHPKYVICNAYDADPRSWAARSILERN
                     PHQVIEGIVLTAYATGASEAYLFTRSLYVEGIASVQKALQEALDANLVGRDILGTDFS
                     CTITVLGVDLGFMGGEETVQIAVIKGRRGMPEQRPPYPAVYGLWDKPTVINSIETLAN
                     VPLIVRDGAAAFASIGTASTGGTKVLTVYDYVPDSEPRLVEVPFGATLREILRHAGYS
                     RAEGELRAVVVGGAEGGALPTSLLDTPYEYDALESVGAIV"
     gene            complement(941..1681)
                     /locus_tag="VGT44_11535"
     CDS             complement(941..1681)
                     /locus_tag="VGT44_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581472.1"
                     /translation="MRGILQGMGLTFSHLARPKVTRMYPYEKRLLPPRSRGLIQLLAE
                     GGLQTEYNLKCESCLLCEKACPPRAITIEYEPMNRWKTRPWFVSYVREGAEKFGTRKM
                     PPASGASKAGYYTQRISEYATEYPNKPVAPCVAMPVKDYLEPEIDLTKVDELLETWPE
                     EAGALSSLLDAVMDVYGYLPQQAAKRIVQVRGVDMSDIFGIATMSPKFKPAPAARGIR
                     RDDQPQRGQSAATRGVYGNINHVRQPNA"
     gene            complement(1685..2287)
                     /locus_tag="VGT44_11540"
     CDS             complement(1685..2287)
                     /locus_tag="VGT44_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEV2581473.1"
                     /translation="MSSNNKEIGNVQGGGKRRPVPIVMAKGPHEYIPGDQELPVQLTS
                     LADMLQWAQNWARSKSVWPLGYGLACCAIEMIAAASAPQYDLSRFGSEVFRSSPRQAD
                     LMIVAGTVSIKMGPRLRLLFEQMPEPKWVISMGQCANSGGEFYDSYYTVQGVDTIVPV
                     DVYVPGCPPRPEALIEGILKLREKIAKQGLKIRGEKEIEV"
BASE COUNT          524 a          777 c          744 g          560 t
ORIGIN      
        1 gactatcgcg ccgactgatt ccagcgcatc atactcgtag ggtgtatcga gcagcgaggt
       61 gggcagcgct ccgccttctg cgccgccgac gacgactgcg cgcaattcgc cttccgcgcg
      121 gctgtagccc gcatgacgca ggatttcgcg cagtgtcgcg ccaaacggca cctctaccag
      181 gcgtggctcg ctgtccggca catagtcata gaccgtcagg actttagtgc cgcctgtcga
      241 agcagtgccg atactggcga atgccgccgc gccgtcacgc acgatcaagg ggacgtttgc
      301 cagcgtttca atcgagttga tgacggtcgg cttgtcccac aggccataga ccgccgggta
      361 gggcgggcgc tgttcgggca taccccggcg acctttaatg acggcaatct ggacggtttc
      421 ttcgccgccc ataaaaccta gatcgacgcc gagaacagta atcgtgcagc taaagtcggt
      481 gcctaaaata tcacgcccga ccaggttggc atccagagcc tcttgcagcg ctttttgcac
      541 ggaggcgatg ccttcaacgt acaggctgcg cgtgaagagg tatgcctcgg acgcgccggt
      601 ggcgtaggcg gtcagcacaa tgccttcgat cacctggtgc gggttgcgct ccagtatgga
      661 gcgcgccgcc cacgaacgcg gatcggcatc gtaagcgttg caaataacat atttggggtg
      721 gtcttcctgg cggcgcgcga tgcgccattt ttcgccggtc agacgacccg cgccgccgcg
      781 cccgcgcaac ttcgcatcca gcgcctgctg gatcagctgc tcgggtgttt gctggcgaag
      841 agctttttcc acggcagcat acccaccagc gcggcgatag gcgtctagcg agcgcacatc
      901 tggctcgccg cgccgccgga caacacgata tacgtcaaat tcaggcattg ggctgcctca
      961 catgattaat attgccgtac acgcctcgtg tcgcggctga ctgtccacgt tggggctgat
     1021 cgtcgcgacg aatgcctctg gcggctggag cgggcttgaa tttcggactc atcgttgcga
     1081 tgccgaagat atcgctcata tcgacgccgc gtacctgcac aatgcgcttt gcggcctgtt
     1141 gcggcagata gccatagaca tccatcacgg catccagcaa cgagctgagc gcgccagctt
     1201 cctccggcca ggtttcgagc aattcatcga ctttggtgag gtcgatttcc ggctctaaat
     1261 agtctttgac cggcatggcc acgcagggcg caaccggttt attgggatat tcggtcgcat
     1321 attcagagat acgctgggta tagtagcccg ctttcgacgc accgctcgcg gggggcatct
     1381 tgcgcgtgcc aaatttctcc gcgccttcgc gcacgtatga aacgaaccag ggacgggttt
     1441 tccagcggtt catcggctcg tattcaatcg tgatagcgcg aggcgggcaa gctttttcac
     1501 agagcagaca cgattcgcat ttcagattat actcggtctg caagccgcct tccgccagca
     1561 actggatcag gccgcgacta cgcggcggga gcaggcgttt ctcataggga tacatgcgag
     1621 taactttggg cctggccagg tggctgaagg tcaggcccat accttgtaaa attccacgca
     1681 ttcgctacac ctcgatctct ttttcgccgc gtattttcaa gccctgcttg gcgatctttt
     1741 cgcgaagctt gaggatgcct tcgatcagcg cctccgggcg aggcgggcag cccggcacgt
     1801 agacatcgac cggcacaatc gtatcaacgc cctgcacggt gtagtagcta tcgtaaaatt
     1861 caccgccgga gttggcgcac tggcccatac tgatgaccca cttgggttcc ggcatctgtt
     1921 caaacagcag gcgcaggcgc ggccccatct tgatcgagac ggtgccggcc acaatcatca
     1981 ggtcggcctg gcgcgggctg gagcggaaga cttccgaccc gaatcgcgac aggtcatatt
     2041 gtggggcaga agctgcggcg atcatttcga tagcgcagca ggccaggcca tagcccagtg
     2101 gccatacgga tttggacctc gcccagtttt gcgcccattg cagcatatca gccaggctgg
     2161 tcaactgcac cggcaattcc tggtcgcctg gaatgtactc gtgggggcct tttgccataa
     2221 caattggcac cggcctgcgt ttcccacccc cctgtacgtt cccaatctct ttgttattgc
     2281 tgctcatcct gtaacactcc cctgcaaagg tttagcagca cctttgcccg gttactgaag
     2341 aatatcattg catttatcac gaatgcaggc aggggaacgg ctccattgtt gtgtggcgta
     2401 aacgtctccc aacctgccct aattatagca cgatgcgcca tagcttgcca taggccgatg
     2461 gctcatatat aatgctggca agatgcaagc ttatgtgagg taatcccaga tgagagaccc
     2521 ggttgaacaa ctccgccgca tccagaacgc cctggtgaaa atagccggtt acgttaacaa
     2581 agggcggcat aaatttgacg aggaa
//
LOCUS       DASZAK010000657         2896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_372763, whole genome shotgun sequence.
ACCESSION   DASZAK010000657 DASZAK010000000
VERSION     DASZAK010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372763"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGT44_11545"
     CDS             <1..415
                     /locus_tag="VGT44_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581474.1"
                     /translation="GLIVALQMLAGFLGLIVLDRLLGRVAPVRLLTIAAWLALVGVVG
                     LLTVHILWITAVSLCVMSLGDSCFYPIVAAESYKRQPGRSGTVRAITSLGQPFEVVLP
                     GIVGLIASQFGLLASLGFLGIAPVLFLLIAPRKAS"
     gene            481..1032
                     /locus_tag="VGT44_11550"
     CDS             481..1032
                     /locus_tag="VGT44_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2581475.1"
                     /translation="MQQHEPATDINADDATLFDRYGQAVFAYLRLHSITREDAEDLLV
                     EVFTAALEQDQLSALPEENRLAWLRRVAHNKLVDSYRRMQRRPIVALGQGEEMLSNVY
                     DDNPERVAIQQESNAQLHQAVGTLPLVQQQVLRLRYGDGLRLVDIAALLNKREEAVRK
                     LHSRALAQLRGIFIPHLRGGDRA"
     gene            1029..2108
                     /locus_tag="VGT44_11555"
     CDS             1029..2108
                     /locus_tag="VGT44_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581476.1"
                     /translation="MIDRDAFTPEEGDEQNQALIGQLRRMYGTRAEDELSLARARVRL
                     LQNSREALPRSPDVKPVQGVHSLPASPQRRREGKRKMQFIDTAIYERRSWQHRLAMIA
                     AVFFIVVLVGSLALILIHRQQSNFANPPQLRPGWTQAAYLSGSGNQTFTHLHIALSTL
                     WGESDSCTGNGNLDIRMQLRDASYEGGGSCPRLTPPSPLIGPDGINLESSSQIVDTIT
                     VKAASSINWYLQLSNADVTPAPVLNQFTAPHSGWTSVGGIGGGYAYSSNGLIKDEGTF
                     DLAETNPSLKALAFVSICVGNGGMTIQVTPPAPGVTFATVVCDGQPHLKMTASHIQNV
                     TVIGDRGVIFQLYVYACTNARGCQS"
     gene            complement(2099..>2896)
                     /locus_tag="VGT44_11560"
     CDS             complement(2099..>2896)
                     /locus_tag="VGT44_11560"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV2581477.1"
                     /translation="LAEGRTTISNAAIEPEIFELITMLRAMGAVIFTSSGRDIRIEGV
                     KQLNGTRMKALGDRIEAASWACLACASDGDITVHGIRPETLGNFLSYYRQVGGGIELV
                     DAGALRFFRQETLRPAFIETDVYPGFSTDWQQPFAILLSQADGISIIHETVHENRFEY
                     LKDLERLGAKTQLAIHCLGGVPCRYSESNFEHSAIIIGPTPFTAVDQMEAPDLRAGLA
                     YVIAAAIARGTTIVTGIEQLERGYGNIVPRLAAMNLNIQRAMIDVQD"
BASE COUNT          618 a          769 c          883 g          626 t
ORIGIN      
        1 tgggctgatt gtagcactcc agatgctggc gggctttctc ggcctgattg tcctggatcg
       61 attgcttgga cgagtcgcgc ccgttcgatt gctgacgatt gctgcatggc tggcactagt
      121 cggggtagtc ggtttgctga cggtgcatat actctggatt acggcggtat cgctttgcgt
      181 tatgagcctg ggagattcgt gcttctatcc cattgtcgcg gctgagtcat ataaacgaca
      241 gccggggcga tcgggcacgg tgcgcgccat caccagcctg ggccagcctt ttgaggtcgt
      301 gctgcccggc atcgtcgggc tgatcgcgag ccaattcggg ctgctggcca gcctggggtt
      361 tctgggaatc gcgcccgtgt tgtttttgtt aatcgcgccg aggaaagcct cctgatgcat
      421 gtcacgtttt tgggggattg agcgatagat agggtgaagt ccgaggacga gaggaattgt
      481 atgcagcaac acgaaccggc caccgacatc aatgccgatg atgccacgct ctttgacagg
      541 tatgggcagg cggtgtttgc ctacctgcgc ctgcacagca ttactcgcga ggatgctgaa
      601 gatttgctgg tggaggtgtt tacagcggcg ctcgagcaag atcagctttc ggcgctgccg
      661 gaggagaatc gcctagcatg gctgcggcgt gttgcgcata acaaactggt tgatagctac
      721 cggcgcatgc agcgtcgtcc catcgttgcc ctggggcaag gcgaagagat gctgagcaat
      781 gtctacgatg ataacccaga acgtgtagcc atacaacaag aatcgaatgc gcagctccac
      841 caggcggtgg gtacgctgcc gcttgtacaa cagcaggtct tacgtctgcg ctatggcgat
      901 ggcctgcgcc ttgtagatat tgctgcgttg ctcaataagc gcgaggaggc ggtgaggaaa
      961 ctgcactcac gcgcgctggc ccaactgcgc ggcatattta taccacactt gaggggaggc
     1021 gatagagcat gattgatcgc gacgcattca caccggaaga gggagacgag cagaatcaag
     1081 cgcttattgg gcaattgcgg cgcatgtacg gcacacgcgc ggaggatgag ttgtccctgg
     1141 ctcgcgcacg tgtacgcctg ctgcaaaaca gtcgggaagc tctaccgcgc tcgcctgatg
     1201 taaagcccgt tcaaggcgta cattcgctac ccgcttcgcc acagaggcga cgagaaggaa
     1261 aaagaaaaat gcagtttata gatacagcaa tatatgagcg aagatcgtgg caacatcgcc
     1321 tggcgatgat tgcagcagtg ttcttcattg tggtgcttgt cggctcgctt gccctgatct
     1381 tgatacacag gcaacagagc aatttcgcca acccgccgca gttgcggcca ggctggacac
     1441 aggcagccta cttgagtggc agtggcaatc aaaccttcac gcacttgcat attgctctct
     1501 caacactctg gggcgaatct gatagctgta caggcaatgg gaatctggat atccgtatgc
     1561 agttaaggga cgccagttac gagggagggg gatcatgtcc aagacttaca ccgccatcac
     1621 cactcattgg cccagatggg atcaacctgg agtcctcatc gcagatagtg gatacgatca
     1681 ctgtcaaagc agccagttcg attaactggt atctgcagct atccaatgcg gacgtgacac
     1741 cggcgccggt tctcaaccag ttcaccgcgc cacattctgg atggacaagc gttggtggca
     1801 taggcggggg ttacgcctat agctcaaatg gcttgattaa agacgaagga acattcgatc
     1861 tagccgaaac aaacccctca ttgaaagcat tggcctttgt ctcaatctgt gtcggcaatg
     1921 gcggcatgac cattcaggtt acgccaccgg caccaggagt gacgttcgcg acagtcgtct
     1981 gtgatgggca gccccatctg aaaatgaccg cctcccacat tcagaatgtc acagtaatag
     2041 gtgaccgagg cgtgatcttt cagctctacg tctatgcctg caccaacgcg aggggctgtc
     2101 aatcctgaac atctatcatg gcgcgctgga tgttcaggtt catggcggcc agcctgggaa
     2161 cgatgttgcc gtagccgcgt tcgagctgct cgatgccggt cacgatggtt gtgccgcgcg
     2221 cgatggctgc tgcgatgacg taggccaggc cggcgcgcag atcgggcgct tccatctggt
     2281 caaccgctgt gaagggtgtc gggccgatga tgatggcgct atgctcgaag ttcgactcgc
     2341 tatagcggca agggacaccg cccaggcaat ggatcgccag ttgtgtcttt gcgcctagcc
     2401 tttcgaggtc tttcaggtat tcaaaacggt tctcgtgtac cgtctcgtga ataatcgaga
     2461 tgccatcggc ctgcgaaagc agtatggcga agggctgttg ccaatctgtc gagaagccgg
     2521 ggtatacgtc ggtctcgatg aaggcgggcc gcagcgtctc ctggcggaag aaacggaggg
     2581 cgccggcatc aaccagttcg atgccaccgc ccacctggcg gtagtaggag aggaagttgc
     2641 cgagcgtctc cgggcgaatg ccatgcacgg tgatgtcgcc atcagaggcg caggccagac
     2701 aggcccacga ggcggcttcg atgcgatcgc ccagcgcctt catgcgcgta ccgttcaatt
     2761 gtttgacgcc ctcgatacga atatcgcgcc ccgacgaagt gaagatgacc gcgcccatgg
     2821 cgcgcagcat ggtaatcagt tcgaagattt cgggttcgat ggctgcattc gagatggtgg
     2881 tgcgcccttc cgcaag
//
LOCUS       DASZAK010000658         3608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_266174, whole genome shotgun sequence.
ACCESSION   DASZAK010000658 DASZAK010000000
VERSION     DASZAK010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266174"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1622)
                     /gene="rny"
                     /locus_tag="VGT44_11565"
     CDS             complement(69..1622)
                     /gene="rny"
                     /locus_tag="VGT44_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV2581478.1"
                     /translation="MQPVIIVLALVILVVGAGVALYAGILLGTARNKQHFAEQIRAEK
                     ETSEQRLLELQAQQREALHEAREESAQFRVSIERDFAERRTELQRQERRLQQKEESFD
                     RKIETFEQRERKLMGRERSLDQARDEIEAIKQEHLKELERIAQLSEEQAQEMLLERIE
                     HRVRTEAARRIRAIEEEAREEADARAREIITLAIQRCASDQVAEAVVSVVPLPNDEMK
                     GRIIGREGRNIRALEAATGVDLIIDDTPEAVILSGFDPVRREVARIALTRLILDGRIH
                     PARIEDVVAKARQEVDIVVREEGERAAMEANVHGLQPELLKLVGRLHFRTSYGQNVLK
                     HSVEVAILATTMAHELGADVNVCKTAALLHDLGKAIDQEVEGPHAIISGEIARRFGKS
                     PKIIHAMVAHHATEAEPQSLEAAIVQAADAISAARPGARRETIDLYIKRLEALESIAN
                     SFTGVEKSFAIQAGREVRIIVKPEEIDEYAANQLASDIAHKIEESLDYPGQIKVCVVR
                     ETRAVDYAR"
     gene            1791..2249
                     /locus_tag="VGT44_11570"
     CDS             1791..2249
                     /locus_tag="VGT44_11570"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV2581479.1"
                     /translation="MSEQQPLRPTHVVTCFLRRVDQDEPRILLVRRSQRVGSYNARWA
                     GVSGFVEEGVPPDEQAYTEIREETGLEREQAHMRRRGAVVEHEDASIGRHWFIHPYLF
                     DVAAPDAIHLDWEATEMRWIAPAELGQFETVPKLQEAYESAMNGDVTTAQ"
     gene            complement(2233..3429)
                     /locus_tag="VGT44_11575"
     CDS             complement(2233..3429)
                     /locus_tag="VGT44_11575"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEV2581480.1"
                     /translation="MDDAVILSAVRTPIGRYAGSLKDVRPDDMAAQIIAEAVRRANID
                     TASIEDVIFGCANQAGEDNRNVARMALLLAGLPVEVAGQTVNRLCGSGLQAVNSAAQA
                     IQTGAGDTFIAGGVESMTRAPFVLGKADGAFSRSMNLQDTTLGWRFINSRLSAMYHPY
                     SMGETAENVAERTKIQREEQDAFALRSHQRAVAAQVSGRFSEEIMPVSIPQKKGDPLM
                     VSSDEQPRADTSLEKLAALKPAFRKDGSVTAGNSSSINDGAAALVLMAGSRAANLGLR
                     PRARIVASAVAGVDPAYMGLGPIPATRKVLQRAGLRIQDIDLVELNEAFAVQSLQCAR
                     ELDIDQEKLNVNGGAIALGHPLGCSGARLVVTLLHELERRGARYGLATMCIGVGQGIA
                     TIIERL"
BASE COUNT          642 a         1116 c         1083 g          767 t
ORIGIN      
        1 ggcgaccctt gcggtcgccc tggcctctac tttgcgcatc cttcctactt tactattaca
       61 atgacagact agcgcgcgta atctaccgcg cgcgtttccc gaaccacgca gactttgatc
      121 tggccgggat aatcaagact ctcttcgatt ttatgagcga tgtcgctggc gagctggttc
      181 gcggcgtatt catcgatctc ttcgggtttg acgatgatgc gcacttcacg gccagcctgg
      241 atcgcgaacg acttttcgac gccggtgaac gaattggcga tcgactcaag cgcttccagg
      301 cgcttgatgt acaggtcaat cgtttcgcgc cgcgcgccgg ggcgcgcggc agagatggcg
      361 tcggcggcct gcacaatcgc cgcttcaaga ctctgcggtt ccgcttccgt agcgtggtgc
      421 gcgaccatgg cgtggatgat cttgggcgat ttcccgaagc ggcgcgcgat ctcgccgctg
      481 ataatggcgt gtggtccttc gacctcctgg tcgatggcct tgccgagatc gtgcagcagc
      541 gcggcagtct tacatacatt tacatcggcg ccgagttcgt gcgccatagt cgtcgcgaga
      601 atggcgacct cgacggaatg cttcaataca ttttgcccgt agctggtgcg gaaatgcagc
      661 cgtcctacca gcttgagcaa ttcgggctgc aagccatgca cgttggcctc cattgccgcg
      721 cgctcgccct cttcgcgcac aacgatgtcc acttcctggc gcgcctttgc cacgacgtct
      781 tcaatacggg ccgggtggat gcgtccatcc aggatcaggc gcgtcagggc gatgcgcgcg
      841 acttcgcggc gcaccgggtc aaagccggag aggatgaccg cctcaggcgt gtcatcgata
      901 atcaaatcga cgccggtcgc ggcctccagc gcgcggatgt tgcgcccttc gcgtccgatg
      961 atgcgcccct tcatctcgtc attaggcagg ggcacaaccg agacgaccgc ctcggccacc
     1021 tggtcggagg cgcagcgctg gatggcaagc gtgatgatct cgcgcgcgcg ggcgtcggcc
     1081 tcttcgcggg cctcttcttc gatagcgcga atgcgccgcg ctgcttcggt gcgaacgcga
     1141 tgttcgatgc gctccagcag catctcttga gcctgctctt cgctgagctg ggcaattcgt
     1201 tcaagctcct tcagatgttc ctgtttgatc gcttcaatct catcgcgcgc ctggtccagg
     1261 gaacgctcgc gtcccatcaa tttgcgttcg cgctgctcga aggtctcgat tttgcgatca
     1321 aacgactctt ccttttgttg caatcgccgt tcctggcgct gcaattcggt gcgtcgctca
     1381 gcgaaatcgc gttcgataga aacgcggaat tgcgccgatt cttcacgcgc ctcgtggagc
     1441 gcctcgcgct gctgtgcctg gagttccagc aagcgctgct cgctggtctc cttctcagcg
     1501 cgaatctgtt cggcaaagtg ttgtttattg cgcgccgtcc ccagcaatat cccggcgtag
     1561 agcgcaaccc ctgctcccac gaccagaatc accagggcga gtacgattat aactggttgc
     1621 acgtcggcct acctccaagt gtaaaaggcg aacaggccct tgttgtcgtt aaaaagatat
     1681 aataaagtgc tggcaataat ggccagcaag ctgttaaccg agcatacgat atgcaagcat
     1741 actatatagg actagtgcta taactgtcaa gcattgagcg aggcgatgac atgagtgaac
     1801 aacaaccctt gcgcccgacg cacgtcgtga cctgctttct tcgccgcgtc gatcaagacg
     1861 agccgcgtat tctgcttgtg cgccgcagcc agcgcgtggg gagctacaac gcgcgctggg
     1921 cgggtgtgag cggcttcgtt gaagagggtg ttccgccaga cgaacaggca tacaccgaga
     1981 ttcgcgagga gacaggttta gagcgcgagc aggcacacat gcgcaggcga ggcgcagtgg
     2041 tggaacacga ggatgcctcc atcgggcggc actggtttat tcacccatac ctgttcgacg
     2101 tagctgcgcc ggacgccatt cacctggatt gggaggccac cgagatgcgc tggatagccc
     2161 ccgcggagct aggccagttc gagacggtgc caaagctgca agaggcgtac gaatcggcga
     2221 tgaacggaga tgtcacaacc gctcaataat cgtggcgatg ccctggccga cgccgatgca
     2281 catagttgcc aggccgtagc gcgcgccgcg ccgctccagt tcgtgcagca gcgtgacgac
     2341 cagccgcgcg ccgctgcagc cgagtggatg gcccagggcg attgcgccgc cgttcacgtt
     2401 gagcttctcc tggtcgatat ccagttcgcg cgcgcactgt aatgactgca cggcgaaggc
     2461 ttcgttcagt tctacaaggt cgatatcctg gatacgcagc cccgcgcgct gcaggacttt
     2521 gcgtgtggcg gggatcggcc ccagtcccat gtaggcggga tcgacgccgg cgacggcgct
     2581 ggcaacgatg cgcgcgcgcg ggcgcagtcc caggttcgcg gcgcggctgc ccgccatcag
     2641 caccagggcg gctgcgccat cgttgatact ggatgagttc ccggcggtga cggagccatc
     2701 tttgcggaaa gccggtttca gggctgccag cttttcgagg gatgtatcgg cgcgcggctg
     2761 ctcatcgctg gagaccatca gggggtcgcc cttcttctgc gggatgctga ccggcatgat
     2821 ctcttcggag aagcgtcccg acacctgggc ggcaacggcg cgctggtggc tgcgcagagc
     2881 gaaggcatcc tgttcttctc gctgaatctt tgtgcgctcg gccacgtttt cggcggtctc
     2941 acccatactg tatgggtggt acatggcgga cagacgagag ttgatgaagc gccagcccag
     3001 cgttgtatct tgcaggttca tactgcggct aaaagccccg tctgccttgc caagcacaaa
     3061 tggggcgcgc gtcatgctct ccacacctcc cgcgataaag gtatcgcccg cgccagtctg
     3121 gatggcctgg gccgcgctgt tgaccgcctg caagccggag ccacacagcc ggttgaccgt
     3181 ctggccagcg acctccaccg gcagccccgc cagcaacagc gccatacgcg ccacattgcg
     3241 attatcctcg ccggcctgat tggcgcagcc gaaaatcacg tcttcgatgc ttgccgtgtc
     3301 gatgttcgcg cgccgcacgg cctccgcgat gatttgtgcg gccatatcat ccgggcgcac
     3361 gtccttgagg ctgcccgcgt agcgcccgat gggggtgcga actgctgata gaataaccgc
     3421 gtcatccatg cccgcttttc cctccttttg gggtctctct ccccataacc tcagcaccct
     3481 cgttcggctt ctttcctgaa acgaggcatt tcgagtgttc agatacgttc gggcgtcttc
     3541 agattggttc atctctgaag ctgtcaatta agctgtcaga aagcgacgct tctcttggcc
     3601 ggtaccct
//
LOCUS       DASZAK010000659         9790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_585405, whole genome shotgun sequence.
ACCESSION   DASZAK010000659 DASZAK010000000
VERSION     DASZAK010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9790
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585405"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VGT44_11580"
     CDS             complement(<1..1124)
                     /locus_tag="VGT44_11580"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2581481.1"
                     /translation="MIETLPAGTELRGRYRIERILGSGGFGHVYLALDTQSKQQFAVK
                     EYLVTGSSGKAQLEHEARVLSKLHHPSLPAFQDAFDELGRYYVVLGYIEGSDLTDCLR
                     MVRQKNETLSYDRILTWLISICDAVTFLHNHQPPVIHRDIKPDNIRITPNGTATLVDL
                     GNAKATEDGARTLFFIRHQGTPGYAPLEQYPGGTGTDERSDVYALGGTLYFALTAHEP
                     PSVSTRNSSISQGLPDLPTMQEILAQNPPETHADPNAGKQFRLGISKPTRPAPRHSRH
                     IAQLASLPPDQLNRLNSIIQRAMAMRPKDRYQTVADMSRDLREVQKALSIARTPPTFQ
                     GRVYDPHSTQPDLQFLVDAWQDNQGTSTPPAQDTPAQPAQP"
     gene            complement(1193..1720)
                     /locus_tag="VGT44_11585"
     CDS             complement(1193..1720)
                     /locus_tag="VGT44_11585"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEV2581482.1"
                     /translation="MAYMEHATTKNSIDEIDQRIIEALQLDGRRPFTRLASELGISEA
                     SVRQRVANLINTQVMQIVAITNPFKMGFSLGGMIGIRVSGDRLLEVAQEIATFDEVIY
                     LIICAGSFDLLAEVVCQDNDHLLNFLTEKLYKVPGVQQTETFMYLRVVKQNNWAFLSQ
                     ARVLPSQFQPPHAER"
     gene            1781..2920
                     /locus_tag="VGT44_11590"
     CDS             1781..2920
                     /locus_tag="VGT44_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II histone deacetylase"
                     /protein_id="HEV2581483.1"
                     /translation="MPAQGTGLVFDERFLQHDTGEQTTVQMRDASFTLAPEPHPSSTT
                     ITRRIKQFLDGSGLTAMMQPIPARAATEEELAAYHTRAYIAGVRAHIAGRPASGAWGE
                     IESDTPLSPGSFEAAMYAAGGAMNAAQAVMEGRARNAYALLRPPGHHAMSNRGMGFCI
                     FNNAVVAAHYLRKTYGLERIMIVDWDVHHGNGTQDAFYADPAVLFVSLHQHNWYPKLA
                     GELEQVGSGAGTGYTVNIPLPAGTGDRGYVAAFEQLVLPVGLQYQPQLIIISAGQDAN
                     WLDPLAQMMLTMAGFRAISRLMRDLAETVCAGRLVMLQEGGYSAAYVPYCTIAAVEPL
                     LGVDLGIVDLYAGSSEVERCETILTRETLRALAQARKWHQQWWKM"
     gene            complement(2931..4040)
                     /locus_tag="VGT44_11595"
     CDS             complement(2931..4040)
                     /locus_tag="VGT44_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV2581484.1"
                     /translation="MTLDSNRLTRLAQLLIRYSLDVQPGHLVAVMADIEALPLVREVY
                     REILRAGAYPELQFEMDDARELYLKEASDEQLTAISPIQQLITEKFDRLLYIDAETNT
                     RRLAHIDPQRAAQQRKAYDALDTRWSQRAAEGSMRWCYTQFPTQAYAQNATMSLAEYW
                     DFLSNACLLNEPDPVTAWQRQSAYQQRLISWLKGRVQVHIIGRDTDLTLSIAGRSFLP
                     CDGHINLPDGEIYSGPVEDSAEGYIRYALPGSYDGHDVDDIRLRFEHGRVVEASAATS
                     QDFLLSMLDMDEGARRIGEFSFGLNYGIREATRNTLYDEKIGGTIHIALGRSYPESGG
                     LNLSALHWDMVCDLRQEGEVWVDGELFLKDGKYIL"
     gene            4209..4529
                     /locus_tag="VGT44_11600"
     CDS             4209..4529
                     /locus_tag="VGT44_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581485.1"
                     /translation="MEPTPRARYDEFADQFTTVLHEHWTDVLQIINNQSPRIAALLKV
                     AVPSGLKRVNGNWHIQLMTTRVVQRDKLREPRDNEVVAQAIRAWAHRAAQLKLPRVTV
                     NFEN"
     gene            4613..5656
                     /locus_tag="VGT44_11605"
     CDS             4613..5656
                     /locus_tag="VGT44_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEV2581486.1"
                     /translation="MQLDWHNYTPGKDATVIRTLDVHAGGEPLRIVTGGMPELPGATI
                     LARRRYMQEHYDHIRRALMWEPRGHYDMYGCLLTSPVTPEADLGVLFMHNEGYSTMCG
                     HGIIGLVTALLETGAIPSKGQHTAVNLDTPAGLVRATAHLDDGHISSVSFLNVPSFLY
                     TRDVALDVADYGRLTIDIAFGGAFYGFLAVEQVGLQVKPLYTAELVKANEAITSAINA
                     QLPVVHPLEADLSFVYGTIFTDLPEDPAHHSRNCCVFANGEVDRSPTGTGVSARVALH
                     AAKGEIADDQVIHIESILGAKSVFSGCIAEQTHVGKYDAVVPEISGRAFITGQHEFII
                     DPSDELGRGFLVK"
     gene            5706..6665
                     /locus_tag="VGT44_11610"
     CDS             5706..6665
                     /locus_tag="VGT44_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HEV2581487.1"
                     /translation="MRIVIPDDYQNAVKSLDCFGKLAGHDVTIYNDTMTDIALLAERF
                     RDAEALVLIRERTAITEPLLELLPNLRFISQTGRGMPHIDIEACTRHSVAIASGGGSP
                     YATAELTWGLVLAAMRYIPQEVENMKQGGWQSALGLGLHGRTLSIYGYGNIGSQVAGF
                     GRAFGMNVLIWGREGSLTRARTDGYTTAGSKAELFSQADVLCLHIKLSEETRGMITGT
                     DLAMMKPSARIINTSRAELLEHGALEAALRAGRPGFAAVDVYEKEPVVDDPLLHMPNV
                     ICTPHIGYVEKDSYELFFSAAFDLLLAYAVGNPKNIVNPQALR"
     gene            6734..7426
                     /locus_tag="VGT44_11615"
     CDS             6734..7426
                     /locus_tag="VGT44_11615"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEV2581488.1"
                     /translation="MEMKTQQGPQSAQEQPVAIGIRFIVVGLVVPLVYQATFTTFAIL
                     APETIFPPQVWLGFHLLTGVFFTCGPLFATGLPLRKAWRALGGRKVAPIDLALVVLIG
                     TAATTALSAAYNAVMNTVFHLSASFGFTQSSQDVPLFVLFVVLVGPLGEEVLFRGYLG
                     VLIRKRWVFILVSAAAWSAAHVDPYNALPLFWTGLMLAWVRQRTGSFYPTFALHFTIN
                     ALALLLHYATPM"
     gene            complement(7433..8329)
                     /locus_tag="VGT44_11620"
     CDS             complement(7433..8329)
                     /locus_tag="VGT44_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEV2581489.1"
                     /translation="MSFDKRQLNGNNGDGRRNGKNMRYRHHKGQRPSTEDERLLNRPT
                     QPLVSPTEATPQEQVKLEEHALERALHFDFTHTDPWRVFRIMSEFVDGFDELAQVPPS
                     IAIFGSARTKPGDPSYEAAVQTAKLLAKAGFGIITGGGPGIMEAGNKGAQEGGNVSIG
                     CNIELPFEQGSNQYLDIALDFRYFFVRKTMFIKYSEAFVIFPGGFGTMDELFEALTLI
                     QTRKVSHFPVILYDSKYWAGLINWIRDTMLPTGKVNPDDLGLLMLSDDPEEICKIVIE
                     AYQESYRQERSNKVSNKEQTIR"
     gene            complement(8371..>9790)
                     /gene="glgB"
                     /locus_tag="VGT44_11625"
     CDS             complement(8371..>9790)
                     /gene="glgB"
                     /locus_tag="VGT44_11625"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV2581490.1"
                     /translation="YCIDMGFTHVELLPITEYPFDGSWGYQVTGYYAPTSRFGTPEDF
                     MFFVDYLHRQGIGVILDWVPSHFPKDGHALNYFDGTHLYEYADTRKGEHKEWGTLVFD
                     YGRSEVCNFLIASALFWLKEYHIDGLRVDAVASMLYLDYLRKSGTWVANEYGGREHLE
                     AVKFLRLLNEAVYGEQPGTVTIAEESTSWPLVSRPTYVGGLGFSMKWNMGWMHDMLHY
                     MKLDPIHRRFHHNDITFSLMYAYSENFVLPLSHDEVVHIKGSMLNKMSGDLWQKFANL
                     RAFYGYMWGHPGKKLLFMGAEFGQWSEWNYKESLDWHLLHYPSDQRHTQLRDYVRELN
                     HLYVLEPALSELDTDPEGFAWVDVHDSDNSVISLMRRSKKREETLVIISNFTPVVRHD
                     YRMGVPYAGDYRELLNSDDIRFGGGGVVNTNLMRSIPGQWQACPHSVILTLPPLATVI
                     VKGYQLLPEIEESLVVGEIEGK"
BASE COUNT         2073 a         2774 c         2777 g         2166 t
ORIGIN      
        1 ggctgtgcag gttgggccgg cgtatcctgc gcgggcggcg tactcgtacc ctggttgtct
       61 tgccatgcat cgaccaggaa ctgtagatca ggctgggtgc tgtgtgggtc gtacacacgc
      121 ccctggaaag tgggcggcgt ccgtgcgatc gatagagctt tttgcacctc ccgcaggtct
      181 ctgctcatat cggcgacggt ttgatagcgg tctttgggtc gcatcgccat agcgcgctgg
      241 ataattgagt tcaggcggtt cagctggtct ggtggcagcg aggcgagctg ggcaatgtgc
      301 cgggaatgac gcggcgctgg cctggttggt ttcgagatgc ccaggcggaa ttgcttgccc
      361 gcgttcgggt ctgcgtgggt ttcgggcggg ttctgcgcca gaatttcctg cattgtcggc
      421 agatcgggca agccctgcga gatcgatgaa ttgcgggtcg agacgctcgg cggctcgtgc
      481 gcggtcagcg cgaagtagag cgtgccgccc agggcataga catccgaacg ctcatctgtg
      541 ccagtacctc ccggatactg ctccaggggt gcgtagccgg gcgttccctg gtggcgaatg
      601 aagaagagcg tgcgcgcgcc atcctcggtg gccttggcgt tgcccaggtc taccagggtg
      661 gctgtgccat tcggcgtaat gcgaatatta tctggcttga tgtcgcggtg aatgacgggc
      721 ggctggtggt tatgcagaaa tgtcaccgcg tcgcaaatgg agatcagcca cgtcaggata
      781 cggtcatagg agagcgtctc gttcttctgg cgcaccatgc gcaggcaatc ggtgagatcg
      841 ctgccttcga tgtatcccaa cacaacatag taacgcccca attcatcgaa ggcgtcctgg
      901 aaggctggca ggctgggatg gtgcaacttg ctgagtacgc gcgcttcatg ttcgagctgc
      961 gctttgccac tcgagcccgt cacgagatat tctttgacgg cgaattgctg cttgctctgc
     1021 gtatctaacg ccaggtagac atgtccaaag ccgccgctgc ccagaatgcg ttcgatgcga
     1081 tagcgcccac gcagttccgt tccggctggc agcgtttcta tcatctgggt tttcctctca
     1141 ttgtgccgct tgttactttt tatagcatta tacaataggc gcagtcaaag cactagcgtt
     1201 ctgcgtgggg tggctgaaat tgggagggca agacgcgcgc ctgggagagg aatgcccagt
     1261 tgttctgttt cacgacgcgc aggtacataa aggtctcggt ctgctgtacc cctggcactt
     1321 tgtagagctt ttccgtgaga aagttgagca ggtggtcatt atcctggcaa accacttcgg
     1381 ccagtaaatc gaaactgccc gcgcagataa tcaaatagat tacttcatcg aaggtagcaa
     1441 tttcctgtgc cacctccagc agccggtcgc cgctcacgcg aataccgatc atgcctccca
     1501 gggaaaagcc cattttgaac gggttggtga tggcgacaat ctgcatcacc tgcgtattga
     1561 tcaggttggc gacgcgctgg cgcacactgg cttccgaaat gcccagttca gacgccagcc
     1621 gggtaaatgg gcggcgaccg tccagttgca gcgcctcgat gatgcgctgg tcgatttcat
     1681 ctatcgaatt tttggtagta gcatgttcca tgtaggccat atactatcat ctaaccaccc
     1741 gcgatgcaat aggatacaat ggtaccaaaa aggagtcagt atgccagctc aaggcacggg
     1801 cctcgttttc gacgagcgat ttttacagca cgataccggg gaacaaacga ccgttcagat
     1861 gcgtgacgct tcgtttacgc tcgcgccgga accgcatcca tcctccacga cgattactcg
     1921 tcgcatcaaa caattcctgg atggctctgg tctaacggcg atgatgcagc ccatcccggc
     1981 gcgcgccgcg accgaagagg aactggctgc ctaccataca cgcgcgtata tcgcgggtgt
     2041 gcgtgcgcat atcgcaggcc gtccagcgag cggtgcgtgg ggagagatcg aaagcgatac
     2101 gcccctcagc ccagggtcgt tcgaggcggc tatgtacgct gccggtggcg cgatgaacgc
     2161 ggcccaggca gttatggagg gccgggcgcg taacgcctac gcgttgctgc gcccacccgg
     2221 acatcacgcc atgagcaatc gcggcatggg cttctgcatc ttcaacaacg ctgtcgttgc
     2281 cgcgcactat ctacgcaaga cctatggcct ggagcgcatc atgatcgtgg attgggacgt
     2341 gcatcatggc aacggcacgc aggatgcctt ctatgccgat cccgccgtgc tgttcgtctc
     2401 gctgcaccag cacaactggt atccgaagct ggcgggcgag cttgaacagg taggtagcgg
     2461 cgcgggcaca gggtacaccg tcaatatccc gctcccggct ggtacaggag accggggcta
     2521 cgtggcagct ttcgagcaac tggtgctgcc tgtcggacta caataccagc cgcagctcat
     2581 cattatttcg gcgggccagg acgccaactg gctcgatccg ctcgcgcaga tgatgctcac
     2641 gatggccggt ttccgcgcga tatcccgact gatgcgcgac ctggccgaga cggtctgcgc
     2701 cggtcgcctg gtgatgctgc aagaaggcgg ctacagcgcg gcctacgtcc cctattgcac
     2761 gattgcagcc gtcgaaccgc tactcggcgt tgacctgggt attgtcgacc tgtatgctgg
     2821 atcatccgag gtcgaacgct gcgagacgat cttgacgagg gaaacactga gggcgctagc
     2881 acaggcgaga aagtggcatc aacaatggtg gaagatgtga tttcctatag tcataggata
     2941 tacttcccat ctttcaaaaa taactcaccg tctacccaca cctcaccctc ttgacgcagg
     3001 tcgcagacca tatcccagtg cagcgcggag agattgaggc cgcccgattc gggatagctg
     3061 cggccaagcg cgatgtggat cgtgccaccg atcttctcgt cgtagagcgt gttccgtgtg
     3121 gcctcgcgaa tgccgtagtt caggccaaag gagaattctc cgatgcgcct ggcaccttcg
     3181 tccatgtcca gcatactcag caggaagtcc tggctcgtgg cggcgctggc ctcgacaaca
     3241 cgcccatgct cgaagcgcag gcggatatca tcgacatcgt gtccgtcgta actgccgggt
     3301 agggcgtaac ggatgtagcc ttccgcgctg tcctcgactg gcccggaata aatttcgcca
     3361 tcgggaagat tgatgtggcc gtcgcagggt aggaaagagc ggcctgctat cgagagcgtc
     3421 aggtcagtat ctctgccaat gatatgcacc tgcacgcgtc cctttagcca ggagatcagt
     3481 cgttgttgat aggctgactg gcgctgccag gcggtgacgg ggtccggctc gtttagcagg
     3541 caggcattac tcaagaagtc ccagtattcc gccagcgaca tagtggcgtt ttgcgcatag
     3601 gcctgcgtcg gaaactgggt gtagcaccag cgcatcgatc cctccgccgc gcgctgtgac
     3661 cagcgtgtat cgagcgcgtc gtaagctttg cgctgctgcg cggcgcgttg tgggtcgata
     3721 tgcgcgaggc ggcgagtatt ggtctccgcg tcgatataga gcaggcggtc gaacttttcg
     3781 gtaatcaact gctggatagg cgagatggcg gtgagttgct cgtcgctggc ctctttcagg
     3841 tagagttcgc gcgcgtcgtc catctcaaat tgcagttcgg gataggcccc ggcgcgcaaa
     3901 atttcgcgat agacttcgcg cacgagcggt aatgcctcga tgtctgccat aactgccacc
     3961 aggtggccag gctgcacatc gagcgaataa cggataagca gctgtgccag tcgtgttaac
     4021 ctgttactgt cgagcgtcat agaactgtac tcccttcgtc tacgtggctt accacaagag
     4081 tagcgcatat tgtgaagaat gtacagaatc atcgaatcaa gacatcaatt gttgagaacg
     4141 aaaaccccgg caaaatatgc tatactttat tcaaccagtc ttactctagt cagacgagga
     4201 ggcgtttcat ggagccaacc ccgcgtgcca ggtatgatga attcgctgac cagtttacta
     4261 cggtgctaca tgaacattgg actgatgtcc tccagatcat caacaatcaa tctccccgta
     4321 ttgcggccct gctgaaagtt gctgtgccat caggcttaaa gcgtgtgaat ggcaactggc
     4381 acatccagct catgacgaca agagtggtgc agcgcgataa actgcgcgaa ccccgcgata
     4441 acgaggtggt ggcccaggcc attcgcgcgt gggcgcaccg cgccgcccaa ctgaagctgc
     4501 cgcgtgtgac ggtaaacttc gagaactgat ccatcctacc gcgattagta caaaaaaaac
     4561 caggtaattc ctatagccac aagctacaca tatttgttac tcggagtgtt tcatgcaact
     4621 cgattggcat aattacacgc ccggcaaaga tgccacggtc attcggaccc tggatgtcca
     4681 cgctggggga gaaccgctgc gcatcgtcac gggtggaatg cctgaactac caggcgcaac
     4741 catcctggcg cggcgacgat atatgcagga acattacgac catatccgca gggcgctgat
     4801 gtgggagccg cgcggccatt acgacatgta tggatgtctt ctcacgtcgc ctgtcacgcc
     4861 agaggccgac ctcggcgtgc tattcatgca caacgagggg tacagtacga tgtgcgggca
     4921 tggtatcatc gggcttgtga cagccttgct tgaaacaggc gcgataccat cgaaagggca
     4981 gcatacggcc gtcaacctcg acacgccagc ggggctggta cgcgctaccg cgcatctcga
     5041 cgacggccat atctcatccg tttccttcct gaatgttcca tcgtttctct acacgcgcga
     5101 cgttgcgctt gacgtagcgg actatggccg attgaccatc gatatcgcgt ttggcggcgc
     5161 gttctatggt tttctcgctg tcgagcaggt cggattgcag gtcaaaccac tatacaccgc
     5221 cgaactggtg aaagcgaatg aagccatcac gtcagccatc aatgcccaac ttccggttgt
     5281 tcacccactc gaagcagacc tcagctttgt ctatggcacc atctttacag acctgcccga
     5341 agacccggcg caccatagcc gcaattgctg tgtcttcgcc aacggtgagg tggatcgctc
     5401 gccgaccggc accggtgttt ctgcccgtgt cgcactccac gccgccaaag gtgagatcgc
     5461 ggacgaccag gtcattcaca tcgaaagcat cctgggcgcg aagagcgtat tcagtggctg
     5521 tatcgctgag cagacccacg tcggcaaata tgatgcagtc gtgcctgaga ttagtgggcg
     5581 agccttcatc accgggcagc acgagtttat aattgacccg agcgatgagt tagggcgagg
     5641 atttttggtg aaataaagta cttcttatag ccataaacta tttttttcct cacggagcac
     5701 cacccatgcg tatcgtgata cccgacgact accagaacgc agtaaaaagc ctggattgct
     5761 tcggcaaact tgccggtcac gatgtaacga tctacaacga caccatgacg gacatcgcac
     5821 tccttgcgga gcgattccgc gatgccgagg cgctggttct catccgcgaa cgcacagcga
     5881 tcaccgagcc actgctcgag ttactgccta acctgagatt catcagccaa acaggcaggg
     5941 gtatgcccca tatcgacatc gaagcttgca cgcggcatag cgtggcaata gcatcgggcg
     6001 gcggctcacc ctacgcgacg gcggaactga catgggggct ggtactggct gccatgcggt
     6061 atatacccca agaagtggag aatatgaagc agggcggctg gcaaagcgca ctggggctag
     6121 gtctgcatgg tcgcacattg agcatctatg gctatgggaa catcggcagc caggtcgcgg
     6181 gctttggtcg cgcattcggc atgaacgtgc tgatctgggg tcgtgaaggt tcgctgaccc
     6241 gcgcacggac agatggctat acgactgccg gcagtaaagc ggagttattt tctcaagctg
     6301 atgtactctg cctccacatc aagctaagcg aggagactcg gggcatgatt accgggaccg
     6361 atttggcgat gatgaagccc tccgcgcgca tcatcaacac tagccgcgca gaactgcttg
     6421 aacatggagc gctagaagca gcgttacgag ccggtcgccc cggttttgca gctgtagatg
     6481 tctatgagaa agagccagta gttgacgatc ccctgttgca catgcccaat gtgatttgca
     6541 ccccgcacat tggctatgtc gagaaggaca gctacgaact ctttttctcg gcagctttcg
     6601 acctgttgct cgcctacgcc gttggaaacc cgaagaatat cgtcaatcca caagctctca
     6661 gataatcttt cgcgaagtat gtcgtaacga acgtgttaca ctttcagcga atacatccca
     6721 gaggaacaca gcgatggaga tgaaaacgca gcagggaccc cagtccgcgc aagagcagcc
     6781 ggttgccatc ggcatacgct tcattgtagt gggcctggtg gtgccgctgg tgtaccaggc
     6841 aactttcacg accttcgcca tcctggctcc ggagacgatt tttccgccac aggtctggct
     6901 aggctttcac ctcctgaccg gcgtgttctt cacctgcggg ccactatttg ccaccgggct
     6961 gccactgcgg aaagcctggc gagcgctcgg tgggcgaaag gtcgcaccca tagatctcgc
     7021 actggtcgtg cttatcggca cagcggcaac cacggcactc agcgccgcgt acaacgcagt
     7081 catgaatacg gtcttccacc tgagcgcaag cttcggtttt acacagtctt cccaggacgt
     7141 accgttattc gtgctattcg tagtgctggt cggtccactc ggtgaggagg tgctctttcg
     7201 cgggtatctc ggcgtactga tccggaaacg ctgggtgttc atcctggtct ccgctgcagc
     7261 gtggagcgcg gcacatgttg acccttacaa cgcgctgccc ctcttctgga cgggcctgat
     7321 gcttgcgtgg gtgcgccagc gcaccgggtc gttttaccca acctttgcgc tgcacttcac
     7381 tatcaacgct ctcgccctgc tgctgcacta cgcaacgccg atgtagtggg cattaacgaa
     7441 tcgtttgctc cttgttactc actttatttg accgctcttg cctatatgat tcctggtagg
     7501 cttcaatcac gatcttgcag atttcttcgg ggtcgtcgga gagcatcagc agacccagat
     7561 cgtccgggtt gactttgccg gtcgggagca tcgtatcccg aatccagtta atcagcccgg
     7621 cccagtattt agagtcgtag agaatgaccg ggaagtggct gacttttctc gtctggatca
     7681 gggtcagcgc ctcgaacagt tcatccatgg tgccaaagcc gccggggaag atcacgaacg
     7741 cctcagaata cttgataaac atggttttcc gcacgaagaa gtacctgaaa tcgagcgcga
     7801 tatctaagta ctggttggac ccctgctcga aaggcagctc aatattgcat ccgatagaga
     7861 cgttgccgcc ctcttgcgcg cccttgttgc cagcctccat gatgcccggt ccgccgccgg
     7921 tgatgatgcc aaatcccgcc ttcgccagca gctttgcagt ctgaactgct gcctcgtagg
     7981 aaggatcgcc gggcttagtg cgcgccgagc caaagatggc gattgagggc ggaacttgcg
     8041 ccagttcgtc aaatccatcc acgaactcgc tcataatgcg gaagacgcgc cagggatcgg
     8101 tatgcgtaaa gtcgaagtgc agggcacgtt ccagcgcatg ttcctccaac ttcacctgct
     8161 cttgtggagt cgcttcagtt gggctgacaa gcggctgcgt cgggcgattc aggaggcgct
     8221 catcctcggt ggagggtcgc tgccccttat gatgtcggta gcgcatattt ttgccatttc
     8281 tgcgtccatc gccattatta ccatttaatt gtctcttgtc aaagctcata cgattactcg
     8341 ctttccgcgc ctgctattcc gcatcttccg ctacttgcct tcgatttcac ccactaccag
     8401 actctcctcg atttccggca ggagttgata gcccttcact atgaccgtcg ccagtggcgg
     8461 caacgtcagg atcaccgaat gtgggcaagc ttgccattgc ccagggatac tgcgcataag
     8521 attggtattc actacccccc cacctccaaa acgaatatca tcactattca gcaactcacg
     8581 gtaatcaccg gcataaggaa cgcccatacg ataatcatga cgcaccaccg gagtgaagtt
     8641 acttataatg acgagcgtct cctccctttt tttactacga cgcatgagcg agatgacgct
     8701 gttatccgaa tcgtgtacat caacccacgc aaagccttcc ggatcggtat ccagttcact
     8761 caatgcaggt tccaggacat aaagatggtt caactctcgc acgtaatcgc gtaactgggt
     8821 gtgacgctgg tcgctagggt agtgcagcag atgccaatcc aggctctctt tgtagttcca
     8881 ctcgctccat tgtccgaatt cagcgcccat gaagagcagc tttttgccgg gatggcccca
     8941 catgtagccg tagaaggcgc gcaggttggc gaacttttgc cagagatcgc ccgacatctt
     9001 attcagcatc gagcctttga tatgcaccac ctcgtcatgc gaaagcggca gcacaaaatt
     9061 ctccgaatag gcgtacatga gcgagaacgt gatatcgttg tggtggaagc ggcggtgaat
     9121 ggggtcgagc ttcatataat gcagcatatc gtgcatccag cccatgttcc atttcatcga
     9181 gaagcccagg ccgccgacgt aggttggtcg gctcaccaga ggccaggagg tcgattcttc
     9241 agcgatggtc acagtccccg gctgctcacc gtagaccgct tcattcaaca ggcgcaggaa
     9301 cttgaccgcc tccaggtgtt cgcgcccgcc gtactcgttg gcgacccatg tgcctgactt
     9361 acgcaggtaa tcgaggtaaa gcattgaggc aacagcgtca acgcgcagcc cgtctatatg
     9421 atattccttg agccagaaca aggcgctggc tatcagaaaa ttgcagacct cgctgcggcc
     9481 atagtcgaag accagcgttc cccattcctt gtgttcgcct ttgcgcgtgt cggcgtattc
     9541 gtacaggtgc gtgccatcga agtagttcag cgcatggcca tctttgggga aatgcgacgg
     9601 cacccagtcg aggatcacgc cgataccctg ccggtgcaga taatcgacga aaaacatgaa
     9661 gtcttctggc gttccgaagc ggctggtcgg ggcgtagtag ccggtgacct ggtagcccca
     9721 ggagccgtcg aaggggtact ctgtgatcgg cagcaactcg acatgcgtaa agcccatatc
     9781 gatgcagtag
//
LOCUS       DASZAK010000660         1949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_372877, whole genome shotgun sequence.
ACCESSION   DASZAK010000660 DASZAK010000000
VERSION     DASZAK010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372877"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1329)
                     /locus_tag="VGT44_11630"
     CDS             complement(<1..1329)
                     /locus_tag="VGT44_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV2581491.1"
                     /translation="MARSSKEELIAQDIKRLHGMGYAQELFRAMGGFSNFAISFTIIS
                     ILSGCVTLFYLVPTTVGFSAASIGWPLVTIFVVIVALGMAELASAYPTAGGLYYWSSK
                     LGGPGWGWFTGWFNVVGQVGITAGIDYGAAVTIDVLLHAWFPSIPATAPAFTNGAYIL
                     DIATQGSTLLIYGLILILHAMLNIFGVRIVAFLNDISVGWHIVFVVVIALGVIIATLA
                     SFQFNPGTAGVCCASQINAHTVGFNNPGSVFAINSNFNFTGFPIWYAFLLSLLLAQYT
                     YTGYDASAHMTEETVGAATRAPWGVVMSVVVSAIAGYILLMSLVAATPDLGAAGSFVN
                     PVLYILESRLGYILGTLLFAAIAIAQLFCGMSSVTSNSRMIYAFSRDGAIPFHNVWHH
                     LDRGRTPRNAIILAAVAAFVLAIPTIFNYTAYFAVTSIATIGLYIAYGLPI"
     gene            1742..>1949
                     /locus_tag="VGT44_11635"
     CDS             1742..>1949
                     /locus_tag="VGT44_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581492.1"
                     /translation="MNDHQWNQPILWDEPDQAIDEWDSSTDMLGVLATTRRVVEVSEL
                     AWVNHDCLAAVASQLRDFKQQEGSQ"
BASE COUNT          502 a          489 c          590 g          368 t
ORIGIN      
        1 gatgggcagg ccatacgcga tatagaggcc gatggtggcg atagaagtca ccgcgaaata
       61 cgcggtgtag ttgaagatcg tcggaatggc cagcacgaaa gcggccaccg cggccaggat
      121 gatggcattg cgtggtgtgc gtcctctgtc caggtggtgc cagacgttgt ggaatggtat
      181 tgccccgtca cgcgagaagg cgtagatcat gcgcgagttc gaggtgacgg aggacatccc
      241 gcagaacaac tgggcaatcg caatagcggc gaagagcagc gtgccgagga tgtagccgag
      301 gcgactctcc aggatgtaaa gcaccgggtt gacgaagctg ccggcagcgc ccaggtcggg
      361 tgttgcggca accaggctca tcaacagaat atagccggcg atggccgaaa ccaccacgga
      421 catcacgacg ccccagggtg cgcgggtcgc cgcgccgacc gtctcctcgg tcatgtgagc
      481 cgatgcatcg tagccggtat acgtatactg ggcgagtaac aggctcaaga ggaaggcata
      541 ccagatcggg aaacctgtga agttgaaatt gctgttgatc gcgaagacgc tgcccgggtt
      601 gttgaaaccg accgtgtgcg cattgatttg cgaggcgcag cagacgccag cagtaccggg
      661 gttgaactgg aaggaagcga gcgttgcgat gatcacgcca agcgcaatta cgacgacaaa
      721 cacgatatgc cagccgacgc tgatatcgtt caagaaagcc acaatacgca caccgaagat
      781 attcagcatg gcatgcagga tcaggatgag gccgtaaatc agcagcgtag acccttgcgt
      841 ggctatgtct aatatgtagg ccccattcgt aaacgcgggg gcggtagctg ggatggacgg
      901 aaaccaggca tgcagcagta catcaatcgt gacagcagcg ccgtagtcga tgccggcggt
      961 aatcccaacc tggccgacca cgttgaacca gccggtgaac cagccccaac ctgggccgcc
     1021 gagcttggat gaccagtagt agaggccgcc tgcggtggga taggcggagg ccagttcggc
     1081 catgcccagc gcgacgatga ccacgaagat ggttacaagc ggccagccga tggaagcggc
     1141 tgaaaagccg accgtcgtgg gaaccaggta aaagagcgtg acacagccac tgaggatgga
     1201 aatgatggta aatgaaatag cgaagttaga gaaaccaccc atcgcgcgaa acagttcctg
     1261 ggcgtagccc ataccgtgca ggcgcttaat atcctgggcg ataagttcct ctttggatga
     1321 acgagccaag agacacctcc cgtttcaaaa tgttcgacgt taaagtgtgg gaagataaaa
     1381 cgacgatggc cagggaaagg cttcattgca tatggacaga cgaagagtga gcgcctcctt
     1441 tcactccatg aaaccatatc gatgttaata ttacacgaaa aatgaatcct gtgtaatggt
     1501 tgtattgtcc acaaacatga gctttccgtc aagtcttttt gaaccaattt gctattcaag
     1561 cggtaaaaat ggtctggaag cgcatgattg agttcccaga ggacgtaccc ttgtggtcgc
     1621 cctctgggac ttcagcgctc attcttaagc cctgtccgca ccggcgtgaa ccattgacga
     1681 atatgcgtat acccgctaca atcgtggcga ggcaatgcaa tcatcgcgag gagcaagctg
     1741 tatgaacgat catcaatgga atcagcccat tttgtgggat gagccggacc aagccattga
     1801 tgagtgggat tctagtacag atatgctcgg cgttctcgct acgactcggc gggtagtaga
     1861 agtgagcgag cttgcctggg taaatcacga ttgccttgcg gccgttgcct cgcagttgcg
     1921 tgacttcaaa cagcaagaag ggagccagc
//
LOCUS       DASZAK010000661         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_336163, whole genome shotgun sequence.
ACCESSION   DASZAK010000661 DASZAK010000000
VERSION     DASZAK010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336163"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VGT44_11640"
     CDS             <1..203
                     /locus_tag="VGT44_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581493.1"
                     /translation="EGGGGCACGGTTHPRASAIKKTAAQIVGMPRGRGRPQGSPPHGV
                     ETLAVALELAQQCYPHRPFIAV"
     gene            complement(209..1363)
                     /locus_tag="VGT44_11645"
     CDS             complement(209..1363)
                     /locus_tag="VGT44_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEV2581494.1"
                     /translation="MANKKVRVAIVGVGNCASSLVQGVQYYRNAQPDDFVPGLMHVNL
                     GGYHISDIEFSAAFDIDQNKVGKDVSEAIFAAPNNTYKFSDVPRLGTKVYRGMTHDGL
                     GKYLSRVITKSKASTDDIVKILEDTGTDVVINYLPVGSETATKWYVEQVLRAKVGLVN
                     CIPVFIAREAYWQERFREVGVPMIGDDIKSQVGATIVHRKLARLFRERGVRLERTMQL
                     NVGGNTDFYNMLERERLESKKISKTNAVTSQLDYDLGEGNVHIGPSDYVEWLSDRKWA
                     YIRLEGRTFGDVPLNVELKLEVWDSPNSAGVVIDAVRMVKLAMDRGISGTLEGPSSYL
                     MKSPPVQHHDDLARELTEAFIKDEGFHAPRQAETLEPTNGYKAEAEIPAE"
     gene            1867..>2641
                     /locus_tag="VGT44_11650"
     CDS             1867..>2641
                     /locus_tag="VGT44_11650"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV2581495.1"
                     /translation="MNRRKRVLVIGLDCAPPELVFEQWRDELPNFKRVMDHGAWGKLE
                     SCIPAITVPAWSSMMSSKDPGTLGFYGFRNRGDYSYEKNTLANSNSVKTDRVWDILSR
                     AGKKVITVGVPQTFPPKPVNGIQVGCFLSPSTKNPERPYTYPASVMQEIEDTVGDYLV
                     DVPNFRTDDKDYLLRQIYTMTEKRFKLVKKWIAQKDWDFFMFVEMGTDRIHHGMWKFH
                     DPTHHKYEVHPVYNESIHNYYRYLDTEIGQILDLIDEDNT"
BASE COUNT          591 a          708 c          717 g          625 t
ORIGIN      
        1 ctgagggggg tggtggatgc gcctgcggcg gcacaaccca cccccgcgcc tcagcgataa
       61 agaagacggc ggctcaaatc gtagggatgc ccagaggacg agggcgaccg caagggtctc
      121 caccccacgg ggtggagacc cttgcggtcg ccctggagct tgcgcaacag tgttatccgc
      181 accgcccgtt tatcgccgtc tagaggggct attcagcggg gatttcagcc tcggctttat
      241 agccattggt tggctctaac gtctcggcct gcctgggggc gtggaagcct tcgtctttga
      301 tgaaggcttc cgtcaattcg cgtgccaggt cgtcgtggtg ttgtaccggc ggcgacttca
      361 tcaggtagga gcttggccct tcgagcgtgc cgctgatgcc gcgatccatc gccagtttga
      421 ccatgcgcac cgcgtcgata actacgcccg ccgagttagg cgaatcccac acttccagtt
      481 tgagttcgac gtttaacggt acgtcaccaa aggtgcgtcc ttccaggcgg atgtaggccc
      541 acttgcgatc cgacagccac tcgacgtagt cggatgggcc gatatggacg ttgccctcgc
      601 cgaggtcgta gtcaagctgg ctcgttacgg cattcgtctt gctgatcttc ttcgactcca
      661 ggcgctcgcg ttccaacata ttgtagaagt cggtgttgcc gccgacgttc aactgcatgg
      721 tgcgctccag gcgtactccg cgctcgcgga agagacgtgc cagcttgcga tggacgatgg
      781 tcgcgccgac ctggctcttg atgtcgtcac cgatcatcgg cactccgacc tctctgaagc
      841 gctcctgcca gtaagcctcg cgggcgatga agacggggat gcagttgacc agcccgacct
      901 tcgcgcgcag cacctgctcc acgtaccact tggtcgcggt ctcggagccg accggcagat
      961 agttaatcac cacatcggtg ccggtgtctt cgagaatctt gacgatgtcg tcggttgaag
     1021 ccttcgactt ggtgatgacg cgcgagaggt atttcccaag gccatcgtgc gtcatgccgc
     1081 gatagacttt tgtacccagg cgcggcacat ccgaaaactt gtaggtgttg ttgggggccg
     1141 cgaaaatggc ctcgctgacg tctttgccta ccttattttg atcgatatcg aatgcagcag
     1201 agaattcgat atcgctgata tggtagccac ctagattcac atgcatcagg ccggggacga
     1261 aatcgtccgg ctgtgcgtta cgataatact gcacgccctg tacgagcgag ctggcgcagt
     1321 tgccgacgcc gacgattgct acacgcactt tcttgttcgc catgtccgtt gttcctttcc
     1381 gggataagcg ctcatcgtgt caggtgcgcc tatccttcta cataggccag ttaaatctgt
     1441 tcatagtata tcgcgtgatc tggctgcctg gctataacaa gcctaacatt gtagctgtga
     1501 agcagcgcac acctcaactt ttgcctattc caggccgatt aatctcaata gaagatgcga
     1561 tgatatagat ttcacggcat tctatgtgac aaatgttgca ggtaacagat ctatcaccca
     1621 taattcctac tcaaacatca agaaaatgca acaagtgtta caatatgacg ggattttgag
     1681 aagtggaaga ccctgcccgg ttaagtaaat gtaacgaggc tgatattttt gcaaccgcat
     1741 ctacacttct tgacaacagt attcgtctac cggcggtgct atgctcaaaa gcacttatga
     1801 ggtggcgaac tgaaaaaaat acgctatagt taagtagaca gacaagtttc tgaaaggcag
     1861 atgtgtatga atcgacgaaa gagagtcctg gtgattggcc tggactgcgc tccgcccgaa
     1921 ctggtattcg agcagtggcg cgatgaactg ccgaatttca agcgagtgat ggatcacggc
     1981 gcgtggggca agctcgaaag ttgtatcccc gccatcactg ttcctgcctg gagttcgatg
     2041 atgtccagca aggaccctgg tacgctgggc ttttatggct tccgcaatcg cggcgattat
     2101 tcttatgaaa agaatacgtt ggccaattca aattctgtga aaactgaccg cgtctgggat
     2161 attctgtcgc gcgcgggtaa aaaagtaatc acggtaggcg tgccgcagac cttcccgccc
     2221 aagccggtca acggcatcca ggtcgggtgt tttctatcac cttcgacgaa gaaccccgaa
     2281 cgtccctata cgtacccggc ttcagtcatg caggagatcg aagacacggt gggcgactac
     2341 ctggtcgatg ttcccaattt ccgtaccgat gacaaagact atctgctgcg ccagatttac
     2401 accatgaccg agaagcgttt caagctggtc aagaagtgga tcgcgcagaa agactgggat
     2461 ttcttcatgt ttgtggagat gggaaccgat cgcattcatc atggtatgtg gaaatttcac
     2521 gatcccacgc atcataagta tgaagtgcat cccgtttata acgagagcat ccacaattat
     2581 tatcgctatc tcgataccga aatcggccag attcttgatc tcatcgacga ggacaatacg
     2641 a
//
LOCUS       DASZAK010000662         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_51529, whole genome shotgun sequence.
ACCESSION   DASZAK010000662 DASZAK010000000
VERSION     DASZAK010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51529"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1385
                     /locus_tag="VGT44_11655"
     CDS             <1..1385
                     /locus_tag="VGT44_11655"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2581496.1"
                     /translation="IQYYHSGSIHDMLAVLIDRPDYRESSMQNMRLNIEQSNQKFHRT
                     FRIFNLIWVFIGFFSITLSSYGVFTNNPGFIHDWHGAVIILLDALTLSIYCMGVFSRI
                     VFHQQGVWPPSFLRAFPFWASLYLSVTLLALIDNSFSWSFFIVLGLSYALFSSRRLIV
                     MVVLAFIGFCVFQGILTLPFTSGNLAALLGIGLSVFSMTVFSMWMQHIITERFERNQL
                     FEQLAQANAELEEAHRRLEESAAQEQELAVLRERTRLAREMHDTLGHALVLISIKLEA
                     AQRLRERDPERCDRELESTKEIARSTMNDLRASIANLRSPALEREPACRAISRYAREM
                     AARTGLRIEYDLHPDIDELPQEVEETLWKVGQEALTNIEKHAHAQHVLLHISRQDRHV
                     ALRITDDGIGLPAHLLEQHTGGDYTCVSPQGHFGLSGMAERVDHAGGHIAIRPGKLGG
                     TSVEVELPLA"
     gene            1812..>2557
                     /locus_tag="VGT44_11660"
     CDS             1812..>2557
                     /locus_tag="VGT44_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein prkA"
                     /protein_id="HEV2581497.1"
                     /translation="MDLVKRLEEYRDRERGLQWEGTFAQYFEIATKQPHIAHLSHERI
                     YHMIMDAGLETTRSGDPNYNFFSQEIFGIEKPLQQIVEYFHSAAQRLEVRKRILLLMG
                     PVGGGKSTIVYLMKRGLEAYSRSDEGAIYALKDCPMHEEPLHLIPNELRADVEREFGL
                     YIEGDLCPHCRYMIDTQYKGHLEDVPVKRIAFSEKYRVGVGTFTPSDPKSQDISELVG
                     GIDLSTIGDVGVESDPRAYRFDGELNIANRG"
BASE COUNT          573 a          729 c          674 g          581 t
ORIGIN      
        1 tcattcagta ctatcattcc gggtctattc acgatatgct agcagtgttg attgatagac
       61 cagattatcg ggaatcgtcc atgcagaaca tgcgcctcaa tatcgagcaa tccaaccaga
      121 agttccatcg tacattcaga atattcaacc tgatatgggt ctttatcggc tttttctcca
      181 taactctctc gagttacggc gtctttacca ataatcctgg tttcatacac gactggcacg
      241 gagcagtcat catcctgctt gacgcgctga cgctcagtat ttattgtatg ggagtattca
      301 gcagaatcgt cttccaccag caaggcgtct ggccgccatc atttttacga gcctttccct
      361 tctgggcatc gctctacctc agcgtcacac tacttgcgct catcgataac tccttttcct
      421 ggtcattctt tattgtgtta ggtctgagct acgccctttt ttcttcgcgc cgactgattg
      481 tgatggttgt tctcgccttt attggcttct gtgtattcca ggggattctc accctcccct
      541 tcacttccgg caacctggcg gccctgctgg gtataggttt aagtgtattc agtatgacgg
      601 tcttcagtat gtggatgcag catattatca ccgaacgatt cgagcgcaac cagctttttg
      661 aacagctcgc ccaggccaat gcagaacttg aggaagcgca ccgccgcctg gaagagtctg
      721 ccgcgcagga acaagagctg gcggtgttgc gcgagcgcac ccgcctggcg cgcgagatgc
      781 acgatacgct cggccacgcg ctggtactga tctcgattaa gttggaagct gcccagcggc
      841 tgcgcgagcg cgatcctgaa cgatgcgacc gcgaactgga gtcgacgaag gagattgcgc
      901 gcagcaccat gaatgacctg cgcgcttcga tcgccaacct gcgctcgcca gcgctggaac
      961 gcgagccggc ctgccgcgcc atcagccgct acgcgcgcga gatggccgcg cgtactggtc
     1021 tgcgcatcga gtatgatctg caccccgata ttgacgagct gcctcaagag gtggaagaga
     1081 cactctggaa agttgggcaa gaggcgctca ccaacatcga aaaacatgca cacgcgcagc
     1141 atgtcctgct ccatatcagt cgccaggatc gtcatgtagc gctgcgcatc actgatgatg
     1201 gcatcggcct gcccgctcat ctgctcgagc agcacaccgg cggagactac acttgcgtca
     1261 gccctcaagg tcatttcggg ctgagcggca tggccgagcg cgttgaccat gccggcggtc
     1321 acatcgctat acgaccgggc aagcttggcg gtacgagcgt cgaagttgag ctgcccctgg
     1381 cgtgaaagct ttgtgaaagc ccctcgattc cccttgacac agacctatct gtacggtatg
     1441 ctacgattac gaacagtgtt tgtatggtgt tttttaggtt tcatctccca cacagtatgg
     1501 aacatgggac cggcattgag aagaccatat catgggagtt cacctcccaa agggtatatg
     1561 aaggaaaggg ttgactctct aactcgcaat gtcgagcggt tcaccttcca tagcgcattc
     1621 gtaaagaaag ggctgggttc tcctactcga tatgtcgagc aagaagccag cccttttgaa
     1681 cacattaagt attccatttc aggaactgtg catcgtcatt atcctgcggg tcagctttcc
     1741 cggcgcgcag gctcagtcgg aagcaatagc gctcacacgc cgctatttgc atagaacaga
     1801 gagggtgact tatggatctg gtgaaacggt tggaagaata ccgagaccgt gaacgaggtt
     1861 tacagtggga aggaacgttt gcccagtact tcgagattgc aacgaagcag ccccatatcg
     1921 cgcatctatc gcacgagcgc atttatcata tgattatgga tgcgggccta gaaaccaccc
     1981 gcagcggcga tcccaactat aatttcttca gccaggaaat cttcggcatt gaaaagcccc
     2041 tgcaacaaat cgtggagtac tttcactccg ccgcgcagcg cctggaagtg cgcaagcgta
     2101 tcctgctgct gatggggccg gtcggcggcg gcaaatctac catcgtctac ttgatgaagc
     2161 gcggcctgga agcctacagc cgctccgacg aaggggccat ctacgccctt aaagattgtc
     2221 ccatgcatga ggagccgctc cacctgatcc ccaacgaact gcgcgccgat gtcgagcgcg
     2281 aattcggcct ctacatcgag ggcgacctct gccctcactg ccgctacatg atcgatacgc
     2341 agtacaaagg ccatttagaa gacgtgccgg tcaagcgcat cgccttcagc gaaaagtacc
     2401 gcgtcggcgt gggaaccttc acgccgtcag acccgaaatc tcaggatatc agtgagttag
     2461 tcggcggcat cgacctctcc accatcggcg acgtaggcgt tgagagcgat ccccgcgcgt
     2521 acagattcga tggcgagctg aacatcgcca accgggg
//
LOCUS       DASZAK010000663         4412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_266379, whole genome shotgun sequence.
ACCESSION   DASZAK010000663 DASZAK010000000
VERSION     DASZAK010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266379"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VGT44_11665"
     CDS             <1..768
                     /locus_tag="VGT44_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV2581498.1"
                     /translation="GADIVFVFAWITLLLAGPLRSGMFTLDGLLVSRLLERFSPGAQV
                     WMGRLTSFFLGVGNARPAPYYAPEVEQVNPAYQQRQPQQVSYKARIAAQRRSLENRRN
                     FLWGIVAGGLGVLGITLVGRVVHFGDDASTPTITNPGSTTSGSGTATGGSSNVIAQTS
                     AVAANSAVTFTIPSNGDPGVLIHLNNGRFVCYDATCTHAGCPVSFDSGSSLLICPCHG
                     AEFDPANNANVVQGPAQTPLTSVPINVNSSTGAITLQ"
     gene            1450..2778
                     /locus_tag="VGT44_11670"
     CDS             1450..2778
                     /locus_tag="VGT44_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV2581499.1"
                     /translation="MPGGRVSPGKRAESGTAMLNAASVQAVNSAKFSQQFSRPLYDSY
                     CFSNLPATVLYLLTGAGRCALPPGTLEGLPTRFDAVMLLFVDGFGWRFFERYAEKYAF
                     LRTFMQQGIVSKMTSQFPSTTAAHVTCIHTGLPVGQSGIYEWNYYEPLVDEMITPLLF
                     AYAGDNTRDTLKRAALPAEMFFPRQTFYQELRALGVASYVFQNAAFTPSTPSNVLFRG
                     ATVAPFKRIDDALQLIARMMATPRASPTYYFLYQNAIDTAGHNYGPNSREFEQEVHVF
                     FTAVEQGLADLLKRNSGKTLLLMTADHGQMEVDPRSTCYLNQQFPSLIPMLRANRQGK
                     ALVPAGSARDMFLHVKDERLDEALALLQRLLKGRAEVYLTRELIAQGLFGAPPLSAEF
                     LARVGNVVILPYRGETVWWYEHGRFDMHFRGHHGGLSPEEMEIPLLAMPL"
     gene            complement(2779..>4412)
                     /locus_tag="VGT44_11675"
     CDS             complement(2779..>4412)
                     /locus_tag="VGT44_11675"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2581500.1"
                     /translation="HTLPDSHVEAFQREASVLASLSHPYILPIFGFGLLYRQEEQLDI
                     TTQLDQDEERGEAKQARCLPYLVLAYAERGSLADVFSREGNRPWSLNRVVTIAREIAD
                     ALDYAHSQGVLHRDVKPANILHMGTHVLLSDFSVSTLIDAEVSHLSTPWAGSPAYMAP
                     EVWQLHPGRYSDQYALAITCFHLLTGDLPLHKDESMRARSWSHVHNFVQPRSLLELRP
                     ELPMAVDFVLQRAMSKNPHDRYPTVTAFATDLLIASQDNTQDLVPMVDTREIGKGARG
                     RVSHAGNNRNTGQEEKLPEKPQPQVEKPFVAASVQRSIPALMSVPSLREPNIALVDAP
                     QTLPHAEPHYQPLQPAQPIRPVQPVQSIQARKPRPTPVKPAPLMEVSTESLAPGRKRD
                     RWTWSAFSLNLFISIGLLAFALVQMQTLQEILTLALALWPALIVGPLLATFFRDIRYR
                     SLSWGLFWGSFFGLANGLFSSLLCLAWFALALFASGLSCSSGCNASGGTNTLLSAIPF
                     VESHALIPVVLGQWVSIFGGALIGIFHIRYDAKQA"
BASE COUNT          912 a         1253 c         1332 g          915 t
ORIGIN      
        1 ggcgctgata ttgtcttcgt ctttgcctgg atcacgctcc tgctggccgg tccattgcgc
       61 agcggcatgt tcacgcttga tggcttgctt gtgtcgcgtt tgctggaacg cttctcgccg
      121 ggcgcacagg tctggatggg acgcctgacc agcttttttc tgggcgtagg gaatgcgcgt
      181 cccgcgccat actatgcacc ggaagtggaa caggtgaacc cggcctacca gcagcgtcaa
      241 ccgcagcaag tcagctacaa ggcgcgtatt gctgctcaaa ggcgctcgtt ggaaaaccgg
      301 cgcaactttt tgtggggcat cgtcgccggc ggtctggggg tactcggtat caccctcgtt
      361 ggccgggtag tgcatttcgg cgacgatgcc agcaccccga ccatcacaaa cccaggtagc
      421 acaacaagcg gatcgggcac ggcaactggc ggctcatcca acgtcattgc gcagacgagc
      481 gcggttgccg ccaacagcgc cgtcaccttc accattccat cgaacggcga tcccggcgtg
      541 ctgatccacc tcaacaacgg cagatttgtc tgctacgatg cgacctgcac ccatgccggt
      601 tgtccagtct cattcgactc cggcagttcg ctgctgattt gcccctgtca cggcgcagaa
      661 ttcgatcccg cgaacaacgc caacgtggtg caggggccgg cgcaaacccc cctgaccagt
      721 gtgccgatca acgtcaattc ctcgaccggg gcgattacgt tgcaataatc gccgaagtcg
      781 atgtgaatac gttgtagcga acgaccgaga ggaccaggta tgcacctggt cctcaatttt
      841 ttcatgtgac ccatctccca tgcacacgac tgaagtcctg atccacatcg atcagaaatt
      901 acgtctagtg ttgggtgtat cctctgcgta cctctgccaa ttcggggcgg atgtcgcctt
      961 cagacattgc agtatccgtg atcggacgtt tctcgagtcg agatgcagcc taatatcgaa
     1021 tatggtcaga cgctcagagg gtcggcctgc ccgcatttac gggcactctt catcaacctg
     1081 aaagactcct ccaagcaagc gaatgagttg cttcatgaac cacatatggc ctccttaaga
     1141 gatgtaagta aggggtgtgt gcggtttctg acggatcaga tcaccgttgt cgcatctctt
     1201 gctccttgtc aaatcgacag aggggatgtg aagtggcctt acacgcgacg acagaagcag
     1261 ggaagaaccg aagctggtcc tgtggggaaa gaagagcatg atgcatcttt ctcgtaatca
     1321 cagtatacca cactaaatgc gtaaatgtca agtatatttt aaattatagt ggaattaata
     1381 ccggcggacg agcaagaggt gttgaagcgc atatgatata ctgaactgca cagcaacgaa
     1441 caccgcagtt tgccgggcgg cagagtgtcg cccggcaaac gggcagaaag cgggacggcc
     1501 atgctcaatg cggcctcagt ccaggcggtc aacagcgcga agttttcaca gcagttcagc
     1561 aggccactgt atgactccta ctgcttttcc aacttgcctg ccaccgtgct ctatctgctg
     1621 accggcgcgg ggcggtgcgc gctgccgcct ggcacgctgg aaggtctgcc cacgcgcttt
     1681 gacgcggtga tgctgttgtt cgtggatggc tttggctggc gtttcttcga gcgctacgca
     1741 gagaaatatg cgttcctcag gacctttatg cagcagggca tcgtttcgaa gatgacctcg
     1801 caattcccct cgacaaccgc cgcgcacgtg acctgcattc ataccggcct gcctgtcggg
     1861 cagagcggca tctacgagtg gaactattat gaaccgctgg tggatgagat gattactccg
     1921 ttgctattcg cctatgccgg agataatacg cgcgatacgt tgaagcgcgc cgcgctcccg
     1981 gctgaaatgt ttttccccag gcagaccttt taccaggagt tgcgcgcgct tggagtggcc
     2041 tcctacgtct ttcagaacgc cgctttcacg ccatctactc cctccaatgt cttgtttcgc
     2101 ggcgcgaccg tggccccatt caaacgcatc gatgacgcgc tgcaattgat cgcgcggatg
     2161 atggcgacgc cgcgtgcgtc gcccacctac tacttcctgt accagaatgc gattgacacc
     2221 gcaggccaca actatggacc gaattccaga gaatttgagc aggaggttca tgtctttttc
     2281 acggcggttg aacagggcct cgcggatttg ctgaagcgca acagcggcaa aacgctcctg
     2341 ttgatgaccg ccgatcatgg gcagatggag gttgacccgc gcagcacctg ttacctgaac
     2401 cagcagtttc cgagtctcat tcccatgctg cgcgcgaacc ggcagggaaa agcgcttgtc
     2461 ccggctggct cggcgcgcga catgttcttg catgtaaagg atgagcgcct ggacgaggcg
     2521 ctggcactgc tccaacggct tctgaaagga cgcgccgagg tctacctgac cagggagctg
     2581 atcgcgcagg gtctgtttgg cgcgccgccg ctttcggcgg aatttctggc gagggtcggc
     2641 aacgtggtga tcctgcccta tcgcggcgag acggtctggt ggtacgaaca cggtcgtttc
     2701 gacatgcatt tccgtggtca tcacggcggc ctgtcgcctg aagaaatgga gatcccgttg
     2761 ttggcgatgc cgctttaatc atgcttgctt tgcatcatag cggatatgga aaatgccgat
     2821 cagcgcgccg ccgaagatcg atacccactg gcctaacacg acggggatga gcgcgtgtga
     2881 ctcaacaaat ggaatagcgc tcaagagtgt gttcgtaccg ccgctggcgt tgcagccgct
     2941 ggagcaggac aggccggaag caaagagcgc cagcgcgaac caggccaggc agagcaggga
     3001 cgaaaacagc ccattcgcca gaccgaagaa gcttccccag aacagccccc acgacagcga
     3061 gcgatagcga atgtcgcgaa agaaggttgc cagcagagga ccaacgatga gcgcgggcca
     3121 taaggccagg gccagggtga ggatctcctg aagcgtttgc atctgtacca gcgcaaatgc
     3181 gagcagcccg atgctgataa aaagattgag cgaaaaggca gaccaggtcc atcggtcgcg
     3241 cttcctacca ggcgcaagcg attcggtgct tacctccata agcggcgctg gtttgaccgg
     3301 cgtcggtcgc ggtttgcgcg cttgtatcga ttgtacaggt tgtactggtc ttattggctg
     3361 cgcaggttgg agtggctggt aatgcggctc tgcatggggc agcgtttgag gcgcgtcgac
     3421 aagcgcgata ttgggctcac gcagcgaggg aacggacatg agcgcgggaa tagagcgctg
     3481 cacgctggct gcgacgaatg gcttttccac ctgcggctga ggcttttccg gcagtttttc
     3541 ctcttgaccg gtattcctat tgtttccggc gtggctcacg cgcccccgcg cgcctttgcc
     3601 gatttcgcgg gtatcgacca tcggaaccag gtcctgcgtg ttgtcttgcg acgcgatcag
     3661 caggtccgtg gcgaaggccg tgacggttgg gtaacggtca tgcggatttt tcgacatggc
     3721 gcgctgtagg acgaagtcaa cggccatggg cagttcggga cgaagttcca gaagcgagcg
     3781 cggctgaacg aagttatgca cgtgcgacca actgcgcgcc cgcatggatt catccttgtg
     3841 caaaggcagg tcgccggtga gcaggtgaaa acacgtgatg gccagcgcat attgatcaga
     3901 atagcgaccg gggtgcagct gccagacctc tggcgccatg tacgcgggcg agccggccca
     3961 gggcgtgctg aggtgtgaca cttccgcgtc gatcagggtg gagacgctga aatccgagag
     4021 caggacgtgc gtccccatat ggaggatgtt ggccggtttg acatcgcgat gcaggacgcc
     4081 ctggctgtgt gcgtagtcca gcgcgtccgc gatctcgcgc gcgatggtca cgacgcggtt
     4141 gagcgaccag ggccgattgc cttcacgcga gaagacgtcc gccagtgagc cgcgctcggc
     4201 atacgccagc accaggtatg gcaggcagcg cgcctgtttt gcctcgccgc gctcctcatc
     4261 ctggtcgagc tgtgtcgtga tatcgagctg ttcctcctgg cgatacaaca ggccaaagcc
     4321 aaaaatgggc aggatgtacg gatgcgacag cgaggcaagc accgaggcct cgcgctggaa
     4381 ggcctccacg tgcgaatccg gcaaggtatg ca
//
LOCUS       DASZAK010000664         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_17053, whole genome shotgun sequence.
ACCESSION   DASZAK010000664 DASZAK010000000
VERSION     DASZAK010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17053"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="VGT44_11680"
     CDS             <1..857
                     /locus_tag="VGT44_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2581501.1"
                     /translation="EQNIAFGMSIQHKPKDYIKRRCAELLELVGLSEKGHSYPHQLSG
                     GQQQRIALARALAVEPKVLLLDEPLSALDAKVRVSLRNEIRRIQQQLKMTAIYVTHDQ
                     EEALAISDRIAVMAKGRIEQLDHPEEIYSNPRTVFAASFVGSSNQFHGVLTSVAEGIF
                     QADHHTLHVPPQSNLKDGERVLVLVRPEEMSIQPATQDGVENVTDTDNQVPGVIELRT
                     FLGPFTRFHVRINESTTLTADVPSQQARDYFVAQNVVLTFPPDACQVLPLDAKEEELE
                     KLAEAETV"
     gene            complement(972..>2845)
                     /locus_tag="VGT44_11685"
     CDS             complement(972..>2845)
                     /locus_tag="VGT44_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEV2581502.1"
                     /translation="TRLLARCGVKTDGANVYEYPTRRATSSYPAAQDGIPRVEVRLEL
                     PDGLSFVERVGFRYCMDKALRASAAAVYWRLVNQIHRQRLWMSACLEELHQADYELSF
                     SHARKIAAVELMEREAVVFPHYALLEGHDRFSRLPQSTARKFQPLHRDSCDFPSPIEL
                     FSKMGVREWFAPLRSRADTETSKRYCIEKESRTLPTLALQVVERRPAGRRAVFDLAVN
                     EQHAFLAGTVAVHNCIGNSGPLPEPVAEAVQENDLVVAAVLSGNRNFEGRIHPQVRAS
                     FLASPPLVVAYALAGTVDIDLTKEPVGTDVNGEAVYLRDLWPSPDEVRDVVAQSITPE
                     LFEHNYAHVFDGDEHWRSLPNTEGELFKWDSNSTYIQEPPFFQNMPLEPEPVKDIRGA
                     RVLAMLDDSITTDHISPAGSFAAASPAGKYLIERGVEKRDFNTYGARRGNHEVMVRGT
                     FGNIRLRNRLTPDTEGYYTVHLPDGEQTTIYEASERYQQEGVPLLVIAGKEYGSGSSR
                     DWAAKGPLLLGVRAAIAESFERIHRSNLVGMGILPLQFKAGENKESLGLTGREVYDIG
                     GIEQGLKPHQEVTVKVTREDGTTFLFQTIARLDSPIDVTYYENGGILLTVLRRLMKE"
BASE COUNT          623 a          871 c          749 g          602 t
ORIGIN      
        1 ctgaacaaaa tatcgctttt ggcatgagca ttcaacacaa gcccaaagac tacatcaaac
       61 gtcgctgcgc tgaattgctc gaattggtcg gcctcagcga gaaggggcac agctatcccc
      121 atcaactctc gggcggccag cagcagcgca tagccctggc acgcgccctg gctgtcgaac
      181 caaaggtact cttgctcgac gagcctctct cggccctcga tgccaaggta cgtgtctcct
      241 tgcgcaatga gatccgccgc atccaacagc agctcaaaat gaccgcgatc tatgtcactc
      301 atgatcagga agaggctctg gccatttctg accgcattgc cgtcatggcc aagggccgga
      361 tcgaacaact cgaccatccc gaagaaattt acagcaatcc acgcacggtc ttcgccgcca
      421 gctttgtagg aagcagcaat caattccacg gtgtactgac atctgtggca gagggtattt
      481 tccaggcaga ccaccataca ctgcacgttc ccccccaatc aaacttaaag gatggcgagc
      541 gcgtattagt gctggtacga cccgaagaga tgtcgataca gccagccacg caggatggtg
      601 tagagaacgt cactgacacg gacaaccagg tccctggggt gatcgagttg cgcaccttcc
      661 ttggtccttt cacacgtttc catgtccgca tcaacgagtc taccactttg acagccgatg
      721 taccaagcca gcaggctcgc gactactttg tcgcccagaa tgttgtgctc acatttccac
      781 cggatgcctg ccaggtgttg ccgctcgatg caaaggaaga agagctggag aaattggctg
      841 aggcggagac ggtatagcta ataagagcaa tattcttaaa caaagaagcg ggcacgattc
      901 ctcgcgcccg ctccctctat aacaacgtcg catcaggagc gtattgaccg caccctcagc
      961 aacgtgtacc cctactcttt catcagcctc ctcaatactg tcaacaagat gccaccattc
     1021 tcgtagtagg tcacatcgat ggggctatcc aacctggcaa tcgtttggaa taagaacgtc
     1081 gtaccatcct cccgggtaac cttgaccgtc acttcctggt gtggcttcag gccttgctcg
     1141 atacctccga tgtcatagac ttctcgtccg gtcaggccga gcgattcttt gttttcgccc
     1201 gctttgaact gcaacggcag gatgcccata cctaccaggt tactgcggtg aatgcgttca
     1261 aagctctcgg cgatggcggc acgcacgcca agcagcaatg gccctttggc ggcccagtca
     1321 cgcgagcttc cagagccgta ctcttttccg gcaatcacga gcagcggaac gccttcctgc
     1381 tggtaacgtt cagatgcctc gtaaatggtt gtctgctctc catcgggcag gtggaccgta
     1441 taatacccct ctgtatctgg agtcaggcgg ttgcgcaggc gaatgttgcc gaacgtgcca
     1501 cgcaccatca cctcgtggtt gcctctacgc gctccgtagg tgttgaaatc acgcttctcg
     1561 acgccgcgct cgatcaggta cttgcctgcc gggcttgccg cggcaaagct gccagcagga
     1621 gaaatgtggt ccgtcgtgat tgaatcgtcc agcattgcta gcactcgcgc accgcgaata
     1681 tcctttacgg gttctggctc gagaggcata ttctggaaga acggtggctc ctgtatatag
     1741 gttgagttgg aatcccactt gaaaagctcg ccttctgtat ttggcaatga gcgccagtgc
     1801 tcgtcgccat cgaacacatg agcatagttg tgctcaaaca attcgggcgt gatcgactga
     1861 gccacaacgt cacgtacttc atccggcgaa ggccataggt cgcgcaggta gaccgcttcc
     1921 ccattgacat ctgtgcccac cggctccttt gtcagatcga tatctactgt gccagccagc
     1981 gcgtaagcca cgacaagcgg tggagaggcc aggaaactcg ctcgcacctg tggatggata
     2041 cgcccctcaa agttgcggtt accactcagc accgccgcca ccacaagatc gttctcctgc
     2101 actgcctcag caactggctc aggcagagga ccactgttgc cgatgcaatt gtggaccgct
     2161 actgtacctg ccaagaaggc atgctgctca ttcacggcca ggtcgaacac cgcccttctg
     2221 cctgctggcc gtcgttccac tacctggagc gccagtgtcg gcagcgtgcg cgattccttc
     2281 tcgatgcagt agcgcttcga agtctctgta tccgcacgcg agcgcaatgg cgcaaaccac
     2341 tcgcgcacgc ccatcttgct gaacagctcg ataggtgacg ggaagtcgca ggaatctcta
     2401 tgcagtggct ggaacttgcg tgccgtcgat tgcggcagtc gcgaaaagcg gtcgtggccc
     2461 tccaatagtg cgtagtgagg gaagactact gcttcgcgct ccatcagctc caccgccgct
     2521 atcttgcgtg catgggagaa cgatagctcg tagtctgcct gatgcagctc ctccagacag
     2581 gccgacatcc agaggcgttg ccgatgaatc tggttcacca aacgccagta caccgccgcc
     2641 gcgctggctc gcagggcctt gtccatacaa tagcggaatc ccacgcgctc cacgaacgag
     2701 aggccgtctg gaagctccaa gcgaacctct actcgaggga taccgtcctg tgctgccggg
     2761 tacgaggaag tcgcgcggcg cgtgggatac tcgtagacgt tggctccatc ggtcttcacc
     2821 ccacagcgcg ccagcagacg ggtca
//
LOCUS       DASZAK010000665         6353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_51635, whole genome shotgun sequence.
ACCESSION   DASZAK010000665 DASZAK010000000
VERSION     DASZAK010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51635"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..804)
                     /locus_tag="VGT44_11690"
     CDS             complement(163..804)
                     /locus_tag="VGT44_11690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2581503.1"
                     /translation="MQGKESMDMGSDLGLPLARTHLSLWPLPVRQEQQQEQARGKQAQ
                     AGKQLIAFDDLYGEYYARVLAYLRFRVGSSEVAEDLVSQVFERVLTHLDGLQAPGAVG
                     AWLFRIARNCATDYFRRQHAAASLDTLVDSSHPRDCSPEETLLAQEERQRLLALLERL
                     PEREREVIGLKFVACLQNREIARVLNMPEGTVGSILYRTLARLRTGLREDEGR"
     gene            953..2176
                     /locus_tag="VGT44_11695"
     CDS             953..2176
                     /locus_tag="VGT44_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEV2581504.1"
                     /translation="MSTNQRDEPTIDNGAASPQTSPALDSDTKRELIAALQQNWQREI
                     EGARTYGDLAQRERDTARRGVLLKMAEAEQRHAQKWERKLAELGAEPPQEQRSLGTRF
                     KGWLRRQLGTEAALRQMEGEEDKDITRYEAQARSINDVEAQAMLREVRRDEESHGRVI
                     REIISPIGPQGMLDVMLRRERWHKRGGGWLGDAIYGANDGLGAVFGIVSGVAGYTGGS
                     TSNLVLISGLAGMLASALSMGSGAFLATKSEREVYQAEINREKHEIEADPEEEKEELA
                     LFYQLKGFTEDESQLLANRLAEQPEQFLRTLAHEELGLSEDSFPKPWVAAVSATISTA
                     LGAFIPIIPFFFLSGFTAIIAAAVISLLAHFTVGAAKTLVTGRSWFNSGMEMTVVGAV
                     EAVITFVLGATFKAV"
     gene            complement(2216..3802)
                     /locus_tag="VGT44_11700"
     CDS             complement(2216..3802)
                     /locus_tag="VGT44_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2581505.1"
                     /translation="MQEYTALATTEEAAIPLAVEMRAITKSWPGVVANDHVNLKVRKG
                     EIHALVGENGAGKTTLMNILYGLIHPDSGEIYIDGQLADIGGPRDAIRLRIGMVHQHF
                     MLIPPLTVAENIVLGHEPGGNNAVYNVRQARELIQNLSKQYGLPIDPDARIEKLSVGL
                     QQRVEILKILYRAADILIMDEPTGVLTPQETDELFRVLRELVDKGKTIIFITHKLREV
                     LELTDNITVLRRGKNAGELVSRDTNQAEIARLMVGREVLLRVNKTPAHPGPVVLHVED
                     IHAQSNRGLEALHGISFDVRAGEILGIAGVEGNGQSELIEALTGMRKITGGHISITQV
                     NEGMAGKTQDLTTLNAREERLVGLAHIPEDRRGSGLVLTDSIEDNLILGRQRWPQFSW
                     NTIVLLLHNIVNWARRLVKEFDVRTSSVEAPARSLSGGNQQKIIIAREFSANPMALIA
                     AQPTRGVDIGAIEFIHRRIIEQRDAGKAILLISAELDEIRSLSDRIAVIYEGKIVDIV
                     SPDATEEELGIMMTGGHIKK"
     gene            complement(3841..4824)
                     /locus_tag="VGT44_11705"
     CDS             complement(3841..4824)
                     /locus_tag="VGT44_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2581506.1"
                     /translation="MFWQIILRSFTSSNTWQTTLEFGSLLLLPALGGVISERSGVVNI
                     AMEGMMLAGAFASVIVTLAMHALGVPNFLCVLIGTLVAMLAGGLMALIHALVSINFKA
                     NQIVSGIAINIIALGLTSYLLYVITPAGQGVPSLPGNLRLPTLPTGPLASIPWLGVVL
                     FQQNVIFYVAILILVATQFILFRTNLGLRIRAVGEHPQAADTAGVNVRLLRYICVLTS
                     GLLSGLAGAYLALGVAGIFNAGMTNGRGFIALAAVIFGKYTPYGAAIGCLVFGLGNGL
                     SPALQDLSSSTNLPNLLSTLPYVLTLIALIGLVGRTTPPAADGVPYDPGSE"
     gene            complement(4827..6173)
                     /locus_tag="VGT44_11710"
     CDS             complement(4827..6173)
                     /locus_tag="VGT44_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2581507.1"
                     /translation="MAVPLMQPETSEQRPPAASRLRRWASALLLSLARPIVAIIFAFI
                     AGAIVILITWPDKTVDPFTNIYNAYTTLLHGSYGNPVNISNTLVQVIPLTLASLSVAI
                     AFRAGLFNIGAAGQLAAGGTVADMIGLTHQSWPSWLLVPTMILAGMAAGAALGGIVGF
                     LKAWRGAHEVVTTIMLNWIVFFSSDYIINSKPFVNPFGTPETTALPTNAQLPSLAALS
                     NQWFHTNFSTFLYSLDVSIFLVIIAVVIYWFIISRTTFGYELRVVGQNPQAARYAGIP
                     IKRTILLVMIIAGAFSGLAGTLNLMGQPGYNLSATAYSGLPTGFDAIAVALLGNTTAL
                     GVVISSLFLGGLRSGTTEMQAVAHVDPNLVLIIEALVLFFIAAEFIPVIRRHLPSWMR
                     SKRHQVLVSSPIGQPALVLPEIEPEDNNASSIDNGVNGMIGEMQSDDGNFTPGKEQ"
BASE COUNT         1346 a         1770 c         1966 g         1271 t
ORIGIN      
        1 gggcgcgtcc agttcgcgca gtggagcgaa cgccgcgcta agctgcccgg caagagggag
       61 gagctcatca tcgtcgggcg acggttcttc catccctgcc gcaatctcgt caaggcgatc
      121 taaccagcga tcatcgacat attcctgttc ctcgcgcatc attcacctcc cttcgtcttc
      181 gcgtagcccg gttcgcaggc gagcaagcgt acgatagaga atagagccaa ccgttccttc
      241 gggcatattg aggacgcgcg cgatctcacg attctggaga cacgctacga acttcaagcc
      301 gattacctca cgctcgcgtt ccggcagacg ctcaagcagg gccagcaggc gctggcgttc
      361 ttcttgcgcc agcagggtct cttccggcga acaatccctg ggatggctgc tatccacgag
      421 ggtgtcgagc gaggctgccg cgtgctgccg ccggaaatag tcggtggcgc agttgcgggc
      481 aatacggaaa agccacgcgc ccaccgcgcc cggcgcttgc aggccatcca ggtgcgtcaa
      541 gacgcgctcg aagacctgcg ataccaggtc ttccgccacc tccgaggacc cgacgcgaaa
      601 gcgtaaatag gccagaacgc gcgcatagta ttccccgtac aggtcatcaa aggcaattag
      661 ctgtttgcca gcctgcgcct gttttccccg cgcctgctcc tgttgttgct cttgcctgac
      721 cggcagcggc cacagagaga gatgcgttct tgccaggggt agccccaggt cgctccccat
      781 atccatagat tctttaccct gcaccggctt ttattctttc gctactggca tagacgtatg
      841 agggaggtat gtattgcatg gttattatga attacgtggg gaagccggtc aagtctcaca
      901 acttgacggt catacttgct cgaacctata ctttgataag gagcgacaat acatgagtac
      961 caatcaaaga gatgaaccga ccatagacaa cggagccgca tccccgcaaa cgtcaccggc
     1021 gctggatagc gacacgaagc gcgaattgat cgccgcgttg cagcagaact ggcagcgcga
     1081 aatagagggt gcgcgcacct atggcgacct ggcacagcgc gaacgtgaca cggcgcggcg
     1141 cggcgtcctg ctcaagatgg ccgaggccga gcagcgtcac gctcagaaat gggagcggaa
     1201 actggccgaa cttggcgcgg agccgccgca ggagcagcgc tcgttgggga cgcgcttcaa
     1261 gggctggctg cgccgccagt tggggacgga ggcagccctg cgccagatgg aaggcgagga
     1321 agacaaggac atcacgcgct acgaggcgca ggcacgcagc atcaatgatg tcgaggccca
     1381 ggctatgctg cgcgaggtgc ggcgcgatga ggagagtcat gggcgtgtca ttcgcgagat
     1441 catcagcccg attgggccgc agggaatgct ggacgtgatg ttgcgccgcg aacgctggca
     1501 taagcgcggc ggcggctggc taggcgacgc catctacggc gcgaacgatg ggctgggcgc
     1561 ggtcttcggc atcgtcagcg gcgtggcagg ttacacgggc ggatcgacca gcaacctggt
     1621 gcttatcagc ggcctggccg gaatgctagc cagcgcgctt tcaatgggtt ccggcgcgtt
     1681 tctggccacc aagagcgagc gcgaggtcta ccaggcggag atcaaccgcg agaaacacga
     1741 gatcgaagca gacccggagg aagaaaagga agaactggcg cttttctatc aactgaaagg
     1801 cttcaccgag gatgaatcgc aactgctggc taaccgtctt gcagagcagc cggagcagtt
     1861 cctgcgcact ctggcgcacg aggaacttgg tctgtcggag gatagcttcc ccaaaccgtg
     1921 ggttgccgcc gtctccgcga cgatatcgac cgcgctgggt gccttcatcc cgatcatccc
     1981 cttcttcttc ctgtcgggct tcacagccat catcgcagcc gccgtcatca gcctcctggc
     2041 gcacttcacg gtcggcgcgg ccaagacgct cgtaacgggc cgctcatggt tcaacagcgg
     2101 catggagatg acggtggtcg gcgcggtcga ggcggtgatc acgtttgtgc tgggtgcaac
     2161 gtttaaggcg gtgtagaggt gcaattgctc ccctacatcc catcattttt attatttatt
     2221 ttttgatgtg gccgccggtc atcataatac cgagttcttc ttcggtggcg tctggtgaga
     2281 cgatatcgac gatcttgccc tcgtagatta cggcgatgcg gtcggagagg gagcggatct
     2341 cgtccaattc ggcggagatg agcaggatgg ctttgcccgc gtcgcgctgc tcgatgatgc
     2401 ggcggtgaat gaactcgata gcgccgatat cgacgccgcg cgtgggctgc gcggcgatca
     2461 gcgccatcgg gttcgccgaa aactcgcgcg caatgatgat cttctgctga ttgccgccag
     2521 agagcgagcg cgccggagcc tcgaccgagg aggtgcgtac gtcaaactct ttgacgagcc
     2581 gcctggccca gttgacgata ttatgcagca gcagcacgat ggtgttccac gaaaactgcg
     2641 gccagcgctg acgccccagg atcaggttat cttcgatgga gtcagtcagc acgaggccgc
     2701 tgccccggcg gtcctccggg atgtgcgcca ggccgacgag acgttcttcg cgcgcattga
     2761 gggtggtcag atcctgggtt ttgcccgcca tgccctcatt gacctgcgtg atcgagatat
     2821 gcccgccggt gatcttgcgc atccctgtta gcgcttcgat caattcagac tggccgttgc
     2881 cttcgacgcc agcgatgccg aggatttccc cggctcgcac atcgaagcta atgccgtgca
     2941 gggcttcgag gccgcgattg gattgggcgt gtatgtcttc gacatgcaag acgacgggtc
     3001 cgggatgggc aggggttttg ttgacgcgca acagtacttc gcggcctacc atgaggcgag
     3061 cgatttcggc ctgattggtg tcgcgggaga caagttcgcc cgcatttttg ccgcgacgaa
     3121 ggacggtaat gttgtcggtg agttcgagga cttcgcgcag cttgtgggtg atgaagatga
     3181 tggttttgcc tttatcgacc agttcgcgca gaacgcggaa gagttcgtcg gtttcctggg
     3241 gggtaagcac gcccgtaggc tcatccatga taagaatatc ggcggcgcga tagaggattt
     3301 tgaggatttc gactcgctgc tgcaggccga ctgagagctt ttcgatacgc gcatcggggt
     3361 cgataggcaa gccgtattgc ttactgaggt tctggatgag ttcgcgcgct tgccgcacgt
     3421 tgtagaccgc gttatttcct cccggttcgt gacccagtac gatgttttcg gccactgtca
     3481 agggtggaat cagcatgaaa tgctggtgaa ccataccgat acgcaggcga atcgcatcac
     3541 gcgggccgcc gatatctgcg agctgcccgt cgatatagat ctcgccgcta tcgggatgaa
     3601 tcaggccgta gaggatattc atgagagtcg ttttgcccgc gccattctcg cccacgagcg
     3661 cgtgaatctc gcccttgcga actttcaaat tgacgtggtc gttggctacg acgcctggcc
     3721 aggatttcgt gatcgcgcgc atttcgactg ccagggggat cgcagcttcc tctgtggtcg
     3781 ccagcgctgt gtattcctgc atacaagctc tcctctattt cgtctaaacc tccagggata
     3841 ttattccgac cctggatcgt agggaacgcc gtcggcggct ggcggcgtgg tacgaccgac
     3901 caggccgatc agcgcgatca gggtaaggac atagggcagc gtcgagagca gattcgggag
     3961 gtttgtcgag ctgcttaaat cctgcaaggc cgggctaagc ccgttgccaa gtccgaagac
     4021 gaggcagccg atggccgcgc catagggtgt atacttgccg aagatcacag cggccagagc
     4081 gatgaaaccc cggccattgg tcatacccgc attgaagatg cccgcgacac ccaacgccag
     4141 gtacgctccg gccaatcccg acagcagtcc gctggtcagc acacatatat aacgcagcag
     4201 tcgcacattg acgcccgccg tgtccgctgc ctgtggatgt tcgccgacag ccctgatgcg
     4261 cagtcccagg ttggtgcgaa acaggatgaa ctgtgttgca accagaatca ggatggcgac
     4321 atagaagata acattttgct ggaacagcac cactccgagc caggggatcg acgcgagcgg
     4381 cccggtggga agggtcggca gacgaagatt gcccggcagc gaaggcacac cctggccggc
     4441 aggggtaatg acgtagagca ggtagctggt caggccgagc gcgataatat tgatggcgat
     4501 gccgcttacg atctggttgg ccttgaagtt gatcgagacc agcgcgtgta tcagcgccat
     4561 cagtccgcca gccagcattg cgacaagtgt gccgatcagc acgcacagaa agttggggac
     4621 accgagcgcg tgcatcgcca acgtgacgat cacactggcg aaggcacccg ccagcatcat
     4681 gccttccata gcgatattga cgacgccgct gcgttccgaa atcacgccgc cgagggccgg
     4741 gagcaggagc agcgagccga attccagcgt ggtctgccac gtattgctgg aagtaaatga
     4801 gcgtagaata atctgccaga acatggttat tgctcctttc caggggtgaa attcccatcg
     4861 tcgctctgca tttcgccaat cattccatta accccattat ctatactact tgcgttattg
     4921 tcctcgggtt caatttcggg taagacgagg gcaggctgtc ctatcggact ggaaacaagc
     4981 acctggtgac gctttgagcg catccagctg ggcaggtgac ggcggatgac cggaatgaat
     5041 tcggcagcga tgaagaacag caccagtgct tcgataatca gcaccaggtt cgggtcaacg
     5101 tgcgcgactg cctgcatttc cgtggtgccg gagcgcagtc cgcccaggaa gagcgagcta
     5161 atcactacgc ccagggcggt tgtattgcca agcagtgcga cggcgatggc gtcaaagccg
     5221 gttggaagtc cgctgtaagc ggtggcagac aggttgtaac ccggctgccc catcaggttg
     5281 agcgttcccg ccaggccaga gaaggctccg gcaatgatca ttaccagcag gattgtgcgt
     5341 ttgataggaa tgccggcgta tctcgcggcc tgcgggttct ggccaacaac ccgtagctca
     5401 tagccgaatg tggtgcgtga gatgatgaac cagtagatta caacggcaat gattaccagg
     5461 aaaatgctga catccaggga gtagagaaag gtgctgaaat tcgtatgaaa ccactgattt
     5521 gagagcgccg ctagcgaggg taactgtgca ttggtgggca gcgccgttgt ttctggagta
     5581 ccgaacggat tgacgaatgg cttgctgttg atgatgtaat cgctgctgaa gaatacgatc
     5641 cagttgagca tgatggtcgt cacgacctcg tgcgcgccgc gccaggcttt gagaaagccg
     5701 acgatgccgc ccagcgccgc gccggcagcc atacccgcca ggatcatcgt agggaccagc
     5761 agccagctcg gccaggactg gtgcgtcagg ccaatcatat cggcgaccgt tcctcctgcg
     5821 gccaattggc cggcggcccc gatattgaag aggccagcgc ggaaggcaat tgccaccgat
     5881 aggcttgcca gcgtcagcgg aatgacctgc accagcgtgt ttgaaatgtt gaccgggttt
     5941 ccgtatgagc cgtgcaaaag cgtcgtgtag gcattgtaaa tattggtaaa aggatcgacc
     6001 gttttgtccg gccaggttat cagaatgacg attgcaccgg cgataaacgc gaagataata
     6061 gcgacgatag ggcgcgctaa tgatagcagg agcgcgcttg cccatctcct gagcctggat
     6121 gcagcaggag gacgctgctc agaggtctca ggttgcatga gtggcacagc catacatctt
     6181 cctccagggc agcaggcggc gataccgcct gaaatagagg gtagcagtgt accctgaaaa
     6241 actgtgcttt aatgataaca ttagatggca taattcacaa gcatggatgc tgctgcggcg
     6301 agtgcgaaaa gggcgaccgc aagggtacgc ccttacaatg actacgaagt ctc
//
LOCUS       DASZAK010000666         8534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_478670, whole genome shotgun sequence.
ACCESSION   DASZAK010000666 DASZAK010000000
VERSION     DASZAK010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478670"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2309)
                     /locus_tag="VGT44_11715"
     CDS             complement(<1..2309)
                     /locus_tag="VGT44_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581508.1"
                     /translation="MSKLSLNVQIAFRQRHTFLALALVFLVLLIAGIVLFHHNIVLLL
                     LLSVLFALIASSAAVLLARPLAIFFYIRSVQAEQRQNSRLYVPLSAMKNMYQTPVHYR
                     DVAKDQRQDVTTLIRQSLTNILFLGLPGAGKTVTLRAYQFAALRDRWRLLFGNKPIPL
                     YVSMREYNVALHEKGILAPSSSAQRSFSPSTPTEMLTTIRTLLTYLLQRSDQAGITYL
                     QPYLEQLIEEGRLLFLCDGLNEVDTDWLGFVSWELMFLMRHTQNKVVMTCRELDYRDR
                     DELRMLVEKNFAAEALILPLTLQQMKLFVTQYIAYSKDEDKDWLYTPEQINDAIEHSN
                     LSYNCNNPMMLLTVMHTMNELGPAQTISLNTRGKLLNSFVLQLIHREQARPQWTMPIQ
                     DEQIMRFLSYVACIGRRAGFRISIPLDKINAPGTGNLRRALPVGEQAALLQNWLDTTI
                     HQLQAQSRQPGNQVEYQPQLSLGDIEYLLDFAQSAALITISHSNILSFRHELIEEYFV
                     AEYLRYLDTLPQGLPFVGELVQSIGLWSEPAAIWAGMLADPMDLARKFAAIGASYSQS
                     NPGYAYNALALSFICAGTTWTSPKSPVETGRQQPQLPGAVRTLLESFVPDQDKCRDLA
                     GIIKRSAKEGGGEVYRSLLPLLIDGNMIDLMLLLDREEVLAFLFNNLHDTVAMGMVDQ
                     TANLVKTLGSFGDDTVNYAAWLLSEPGLTSDLHATILDILGRTASQRAVRLLCNRLEI
                     EEERIQKAVVNALVVLGPALVLEAIVDEL"
     gene            2422..3180
                     /locus_tag="VGT44_11720"
     CDS             2422..3180
                     /locus_tag="VGT44_11720"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEV2581509.1"
                     /translation="MKQLPGTGAFDIQAKSWQYLRPAEADFCPRSMAMPFELPLFPLN
                     VVLFPGADLPLHIFEPRYRQMINECYQQQKPFGIVLARPETQSLLEEPHHIGTLAEIE
                     TLARLEDGRMNLIARGGQRFRIVGQHRKKPYLTGIVEVYEDRSEPEQTSREQGERARD
                     LFTSYLNILLEVVGKEEVQFDLPELPGELSHFIAYFLDLENDQKQALLELDSTVERLK
                     SEIDILRREVPFMRQMLSLSSLYRAGSPDRSKLN"
     gene            3197..3502
                     /locus_tag="VGT44_11725"
     CDS             3197..3502
                     /locus_tag="VGT44_11725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581510.1"
                     /translation="MTDVVRFRQQLEAVLRTRDIQQVSEFLIAEKQWIPGTAMPDDPD
                     FAMWMMIAGNAHLRDLHEQARQWLVNHGHAEEATMLVARGKGEKAKRSRAPIVKKGR"
     gene            3557..5641
                     /locus_tag="VGT44_11730"
     CDS             3557..5641
                     /locus_tag="VGT44_11730"
                     /inference="COORDINATES: protein motif:HMM:NF042625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HEV2581511.1"
                     /translation="MPAIGQLAALTVVACLLLWPAPVTQNQLFAAWLDSDLMLSHWPT
                     ALLIQRTFAQSHSLPLWNPYFGGGQPLGADPLAALFYPPTHLVHFFSLRDYYLVLILG
                     HLVWAGVGMLLLARRALGLPRLPALVAAISYMATPALLGHLGAGHITIVQTVAWYPWL
                     ALACWATVRDPFRWGALLGLCIALTLLAGHPQMAYYGLLMTVILAAWLLAKRWRQDGW
                     RALLASIGGLGLAGIIGVLLAAIHLLPLMEFTALSTRRAQVIIYDTYPIQNFLHALVY
                     QPSPNGLRWEDTISPGLVVLVLAFVGLVTRLRTVWPLILAIVLVAALAIGYASPIYPF
                     AVRFLPDLNRFRDIDRVWFVALILISLLTGFGADALMRGARRILNRGGKHISRFGQMA
                     AGLFAVLLVAFSLVVSDFGYARVGDINAITTPTALANAATRLAGSGRVYGAQENIMQL
                     YAAELQVPFADGLNPLMIESYVTYMLRADGLSGGGYYLHIPIPSPSARPDARLLGLMN
                     VSVVVSRTRLSDPHFVQVGEADGTLIYRNTADAGPGYLVRPGPGGNAPTLTGVQRLNE
                     SVRVVTLAPGQETFTFTTNSVSYFVIASPSFPGWIANVDGHDATIQQFAGALPAIKVG
                     PGTHTLSYTYAPSSVRTGAILSLVGLLAALLWLVFAWLRKAGQPGWPWKHESEITALQ
                     GKDNDKKYAQVS"
     gene            complement(5844..7895)
                     /gene="fusA"
                     /locus_tag="VGT44_11735"
     CDS             complement(5844..7895)
                     /gene="fusA"
                     /locus_tag="VGT44_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV2581512.1"
                     /translation="MKVYTAEHIRNVALISHVGAGKTSLIDAALYDSGAVTRQGKVDD
                     GSSVADYDPDELKRRMTLHAKALPVEWKNTKINFIDTPGYADFVGEVKAGLRVADAAL
                     VVVTAEKGVEVGTELTWQYADERKLPRIVLVNKLDRENTSFDTALESLRKQFGLKVVP
                     LQLPIGEQAGFKGVIDLVTNKAYTFEGGNKVQEVPIPADLQDSIGSYREQLIESAVES
                     DDAIMEKFLEGEELSDAEILSVVKQGTRTGQLIPVLCGAGSKNIGVQTLLDAIVDYLP
                     SAEDAASEDAKAFGNATSLFVFKTMAAQVGTISNFRVYSGTLKSDSHLTNLQTRADER
                     IGQISIQRGKSQENAAEIAAGDFGAVAKLSNTHTGDTLSSNKDATTTLEPINFPEPCY
                     TVAVFPRSKADLDKMSSALARVAEEDRTLRVSRDPQTAEVLLSGIGESHLQIVLEGIK
                     RKYGVDLEARDPRVSYRETIRKKVRANGRHKRQSGGHGQFGDVWLEIEPLPMGGEENF
                     IFEDKIVGGVVPGQYIPGVEKGVRDSLKRGFVSGNPLVYIKVALVDGKYHPVDSSAQS
                     FEIAASLGMQEAVPQASPTILEPVVTATITVPESYMGDIMSDINTKRGRVLGMSSLGN
                     GQQQITATVPQAEMLHYATDLRSITQGRGSFKTEFYQYEEVPANIQQEIIAQHKKAQE
                     A"
     gene            complement(7898..>8534)
                     /locus_tag="VGT44_11740"
     CDS             complement(7898..>8534)
                     /locus_tag="VGT44_11740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV2581513.1"
                     /translation="TGGDSPDNLITLCETCHDHIHRTRQEHKIERKSRGWRDATHMGM
                     IRWRIYEQAKAMFPNVHLTYGYITKQTRITNNLEKSHLIDARCISQHPLVGSDGTWYL
                     IKYVRRNNRQLHKATIRKGGKRQRNTAPKYVHGFRLFDCVQYQGTFCFVFGRRSSGYF
                     DLRTLDGTKIHAGASYKHLKSVQRASAHLIERRSGISSPPINGDGSSCRDI"
BASE COUNT         1712 a         2437 c         2415 g         1970 t
ORIGIN      
        1 tttagttcat ccacgatggc ttcgaggacc agcgccggtc ccagcacaac aagggcattg
       61 acgactgctt tctgaattcg ctcttcctcg atttccaggc gattgcagag cagtcggaca
      121 gccctttgag aggctgtccg accaagtata tcaagtatcg tcgcgtgcag gtcggaagtc
      181 aggccaggct cagagagcag ccacgcggca taattgaccg tgtcgtcacc aaaacttccc
      241 agggttttca ccaggtttgc ggtctgatcg accatcccca ttgcgaccgt atcgtgcagg
      301 ttgttgaata aaaacgccag cacctcttcc cggtcaagca aaagcatcag gtcgatcata
      361 ttgccatcga tcagcagcgg cagcagcgag cgatacacct cacccccgcc ctctttggca
      421 ctgcgcttga taatgcccgc gaggtctcgg catttgtcct gatccggcac aaaggactcg
      481 agcaatgtgc gcactgcccc cggaagttgt ggctgttgtc ggcccgtctc cacaggcgat
      541 ttgggcgaag tccaggtggt tcctgcgcag ataaagctga gcgcgagcgc gttatacgca
      601 tagccgggat tgctctggct gtagctcgcg ccgatagctg cgaacttgcg agccaggtcc
      661 atcgggtccg ccaacatgcc tgcccagatt gcggcaggtt cgctccagag accgatggac
      721 tgcacaagct cgcctacgaa tggcagtccc tgtggcaggg tatccaggta gcgcaggtat
      781 tccgcgacga agtattcttc gatcagttcg tgacgaaaac tcaggatatt agagtgactg
      841 atggtgatga gtgcggcgct ttgcgcgaaa tccagcaagt actcgatgtc gccgagtgaa
      901 agctggggct gatactcgac ctggttgccg ggctgacgtg attgtgcctg gagctggtgg
      961 atggtcgtat caagccagtt ctggagcagc gcggcctgtt ctccgactgg gagcgctctg
     1021 cgcaggtttc cagtccctgg cgcgttgatt ttgtcgagcg ggatggagat acggaagccg
     1081 gcgcgccgcc caatgcaggc aacatagctc agaaagcgca tgatctgctc atcctgaata
     1141 ggcatcgtcc attgtggccg agcctgttcc cggtggatca attgtaacac gaagctgttg
     1201 agcagcttgc ccctggtatt caggctgata gtctgagccg gacccaactc gttcatggtg
     1261 tgcatcaccg tgagcaacat catgggattg ttgcagttgt aactcagatt gctgtgttcg
     1321 atggcatcgt tgatctgctc gggagtgtag agccaatcct tgtcttcatc tttcgagtag
     1381 gcaatgtatt gtgtgacgaa gagcttcatc tgctgcagcg tgagcggcag aatcagcgct
     1441 tcggcggcga agtttttctc gaccagcatg cgcaattcat cccggtcgcg atagtccagt
     1501 tcgcgacagg tcatcacgac cttattctgc gtgtggcgca tcaaaaacat gagttcccag
     1561 ctcacaaacc ccagccaatc ggtatcaact tcgtttaatc cgtcacataa gaagagcaag
     1621 cgcccctctt cgatgagttg ttccagatat ggctgcaaat aggtgatacc tgcctgatcg
     1681 ctcctctgga gcagatatgt gagcaaggtc ctgatcgtag tcagcatttc agtgggggtc
     1741 gatggcgaga atgagcgctg ggcgctgctc gagggagcga gaataccctt ctcgtgcagt
     1801 gcaacgttgt attctcgcat cgaaacatac aaaggaatgg gcttgttgcc aaagagcaac
     1861 ctccacctgt ctcgcagtgc agcgaattga tacgctcgca gcgtgacggt cttgccagct
     1921 cctgggaggc caagaaacag aatgttggtg agagattgcc ggatgagcgt cgtcacatcc
     1981 tgtcgctgat cctttgccac atcacgataa tgaaccggcg tctggtacat gttcttcatc
     2041 gcgctcaatg gcacgtagag cctgctgttc tggcgttgct ctgcttgcac gctgcgaata
     2101 tagaagaata tcgctagcgg tcgagccaga aggacggcgg cactgcttgc tatcagcgca
     2161 aagagcaccg aaagcagcag caggagaacg atgttgtgat gaaacagcac aatcccggcg
     2221 attaacaaca ccagaaaaac cagggcgagc gctagaaacg tgtgtcgttg ccggaacgca
     2281 atttgaacat taagggataa tttcgacata actcactcac cacaataatt tgaatagtga
     2341 tacagtgagt atatcgcacg gtatctatca tgacaagcat gagtgcagat aggtttcact
     2401 cttatcgtac aacgtggtat catgaaacaa ctaccaggaa caggagcgtt cgacatacag
     2461 gcaaagtcct ggcaatattt acgcccggcg gaggcggatt tctgcccgag gagcatggcg
     2521 atgccgtttg aactaccctt atttccgtta aatgttgtgc tttttccagg ggcggacctg
     2581 ccgctgcata ttttcgagcc gcgctatcgt cagatgatta acgagtgcta ccagcagcaa
     2641 aagccatttg gtatcgtgct ggcgcgtccg gagacccagt cgctgctcga agagccgcat
     2701 cacataggca cattggccga aatcgagacg ctggcgcgcc tggaagatgg ccgcatgaac
     2761 ctgattgcca ggggtggcca gcgctttcgc atcgttggcc agcatcgcaa gaagccctac
     2821 ctcaccggta tcgtagaagt gtacgaggac cgcagtgagc cggagcaaac ctcgcgcgag
     2881 cagggggaaa gggcgcgcga tctctttacc tcgtacctca acatcctgct cgaagtcgtc
     2941 ggcaaggagg aggtccagtt cgatttgccg gaactccctg gcgagctttc acactttatc
     3001 gcctatttcc tcgacctgga aaatgaccag aaacaggcat tactggaact tgattcgacg
     3061 gtcgaacgtc ttaaaagtga gatcgatata ctacggcgcg aagtaccgtt catgcgtcag
     3121 atgttgtcgc tgagcagtct ctaccgcgcc ggcagccccg atcgcagcaa gctgaattaa
     3181 tgtggagcga aatacgatga ccgatgtagt ccgctttcgc cagcagcttg aagccgtcct
     3241 gcgcacacgc gatatccagc aggtcagcga atttcttata gccgaaaagc aatggattcc
     3301 cggaacggcg atgcccgatg atcccgactt cgctatgtgg atgatgattg ctggcaacgc
     3361 gcatttgcgt gatctccacg agcaggcgcg gcagtggctg gtgaatcatg gccatgcgga
     3421 agaggcaact atgctggtgg ccagggggaa aggagaaaag gcaaagcggt cacgtgcgcc
     3481 gatcgtcaaa aaagggagat gaatgagtaa gctcacagcc atgcgccaat atttgaatag
     3541 acgtaaggcg tcatggttgc cagcgatagg tcaactggcc gcccttaccg ttgtcgcctg
     3601 tttgctgctc tggcccgcgc ctgtgacgca gaatcagtta tttgccgcct ggctcgacag
     3661 tgacctgatg ctcagccact ggcccacggc gcttttgatc cagcgcacgt tcgcccaatc
     3721 gcattcgctg ccactctgga atccatactt tggaggcggt cagccacttg gagccgatcc
     3781 cctcgcggcg cttttctacc caccaaccca ccttgttcac tttttctcgc tgcgtgatta
     3841 ctacctggtc ctcatcctcg gacacctggt gtgggcgggc gtgggaatgc tactgctggc
     3901 gcgccgcgcg cttggcctgc cgcgcttgcc cgcgctcgtg gcggccatca gctacatggc
     3961 aacgcctgct ctactgggtc atctcggcgc gggccatatc accatcgtcc agacggtcgc
     4021 ctggtatccc tggctcgcgc tggcctgctg ggcgacggtg cgagacccct tccgttgggg
     4081 cgcgctgctg ggattgtgta ttgcgttgac gctgctggcc ggccacccgc aaatggcgta
     4141 ctacggcttg ctgatgaccg tgatcctggc cgcctggctg ctcgcaaagc ggtggcgaca
     4201 ggatggctgg cgcgcgctgc tcgcgtcgat tggcgggtta gggctggcgg gcatcatcgg
     4261 cgtattgctg gcggcgatcc acttattgcc actcatggaa tttacggcgc tttccacccg
     4321 ccgggcgcag gtgattatct atgataccta tcccatccag aacttccttc acgcgctggt
     4381 ctaccagcca tcgccgaacg gactgcgctg ggaagacacg atctcccccg gcctggtcgt
     4441 gctggtcctc gcattcgtcg ggctggtgac acgcctgcgc acggtctggc cgctaatact
     4501 cgcaatcgtg cttgtcgcgg cgctggcaat cggatacgca tctccaatct acccattcgc
     4561 ggtgcgtttc ttacccgatc tcaatcggtt tcgcgatatt gatcgcgtct ggtttgtcgc
     4621 gctcatactg atctccttgc tcaccggctt tggcgcggac gctctcatgc gcggcgctcg
     4681 acgtattctc aatagaggtg gtaagcacat ttctcggttt ggtcaaatgg cagccggttt
     4741 gttcgctgtt ctgctcgtgg ccttctcact ggtcgtctca gatttcggtt atgcccgcgt
     4801 cggcgatatc aacgccatca ccactcccac tgcgctcgcg aacgctgcga cacggttggc
     4861 gggtagtggt cgtgtctacg gtgcgcaaga aaatatcatg caattatacg cggcagaact
     4921 gcaggtgccg tttgccgatg ggcttaatcc tcttatgatc gaatcctatg tcacctacat
     4981 gctgcgagcc gatggtttgt ccggcggtgg ctattatctc catatcccca taccatctcc
     5041 ctccgccagg ccggatgccc ggctgctcgg attaatgaat gtgagcgtcg tggtctcgcg
     5101 cacacgcctg agcgacccac actttgtgca ggtgggtgag gctgacggca cgttgatcta
     5161 ccggaacacc gccgatgctg gccccggcta cctcgttcga cctgggccgg gcggcaatgc
     5221 gcccacgctc accggagtgc agcgactcaa cgagagtgtg cgcgtcgtca ccctggcgcc
     5281 tgggcaagaa accttcacct tcaccaccaa ctcggtctcg tactttgtga tcgcttcccc
     5341 ctcgtttcct ggctggatcg ccaatgtaga tggtcacgac gcgaccatcc agcagttcgc
     5401 gggagcgctg cccgccatca aggtcggtcc aggcacacac acgctgagct atacctatgc
     5461 gccttcatca gtccgcacag gcgcgatact atcgctagtt ggtctgctgg cagcgcttct
     5521 gtggctcgtt ttcgcatggc tcaggaaggc gggccagccg ggctggccct ggaagcacga
     5581 gagcgaaata acggcgctcc aaggcaagga caatgataag aagtacgccc aggtcagttg
     5641 acctgggcgt attgcggatc aaagtcagtt ggcccgaccc gcgagtggag ttcgcttcct
     5701 cggcgcataa cgcggtctga ttcagccgga acgaactgga accggctgcc tctttccgca
     5761 ggtgcgcctg gtcgcatact tcaccattgc gttcgatagt cgcagagact atcgaacata
     5821 tgtattaacg aacaagacga gcttcaggct tcttgcgcct tcttgtgttg cgcaatgatc
     5881 tcttgctgga tgttcgcggg tacttcttcg tactggtaga actccgtttt gaaggagccg
     5941 cgcccctggg taatcgagcg gaggtcagtg gcgtaatgca gcatttcggc ctgtggcacg
     6001 gtggcagtga tctgctgctg cccgttgccc aacgatgaca tgccgagtac gcgcccgcgc
     6061 ttggtattga tatcgctcat gatatcgccc atgtaggatt ctggcaccgt gatggtagca
     6121 gtcacgaccg gctccaggat ggtcgggctg gcctgtggca cggcttcctg cattccaagc
     6181 gaggcagcga tttcaaacga ttgcgcggat gagtcaacgg ggtggtactt gccgtccacc
     6241 agggcgacct tgatgtacac cagcggattg ccggagacga aaccgcgttt gagcgaatct
     6301 cgcacacctt tctcgacgcc tgggatgtac tggccgggaa ccacaccgcc gacgatttta
     6361 tcttcgaaga tgaagttctc ctcgccgccc atcggcagag gctcgatctc caaccagaca
     6421 tcgccgaatt gcccatgccc accgctctga cgtttgtggc ggccattggc gcgcaccttc
     6481 ttacggatgg tttcgcgata ggagacgcgt gggtcgcggg cctccaggtc aacgccgtat
     6541 ttgcgtttaa tgccttcgag gacaatctgc aaatgcgatt cgccgatgcc ggagagcagc
     6601 acttcggcgg tctgggggtc gcgcgagaca cgcagcgtgc ggtcctcttc ggcaacgcgc
     6661 gccagggcgc tgctcatctt gtccaggtcg gccttgctcc tggggaaaac ggcgaccgtg
     6721 tagcacggtt cggggaagtt gatcggctca agcgtagttg tcgcgtcctt gttgctgctt
     6781 aaggtatcgc cggtatgcgt attggagagc ttggcaaccg cgccgaagtc tcctgccgcg
     6841 atttcggcgg cgttttcctg gcttttgccg cgctggatac tgatctgtcc gatacgctca
     6901 tcggcccggg tttgcaggtt cgtgagatgt gaatccgatt tcagtgtgcc actatacacg
     6961 cggaaattgc taatcgtgcc gacctgggcc gccatcgtct tgaatacgaa cagtgacgta
     7021 gcattaccga atgcctttgc atcctcagac gccgcgtcct ccgcgctggg aagataatca
     7081 acgatagcgt cgagcagcgt ttgcacgccg atattcttgc tgcccgcgcc gcacaaaacc
     7141 ggaatgagct ggccggtgcg cgtcccctgc ttgacgaccg agaggatttc tgcatcgctc
     7201 agctcttcgc cttccaggaa tttctccatg atcgcgtcgt cgctctcaac ggcggactcg
     7261 atcagttgct cgcggtagga accgatgcta tcctggagat cagccgggat ggggacttcc
     7321 tgcaccttgt tgccgccctc gaaggtatag gccttattcg tcaccaggtc gatgacgccc
     7381 ttgaagcctg cctgctcgcc gatggggagt tgcagcggca cgaccttcag accgaactgc
     7441 ttgcgcagcg actccagggc agtgtcgaag gaggtatttt cacggtccag cttgtttacc
     7501 agcacgatac gcggcagctt gcgctcatcg gcgtattgcc aggtgagttc tgtaccgact
     7561 tcgacgcctt tctcggctgt gacgacgacc agcgcggcat cggccacgcg cagacccgcc
     7621 ttgacctcgc ctacgaagtc ggcgtaaccg ggggtatcga tgaagttgat cttggtgttt
     7681 ttccattcga cgggcagcgc cttcgcgtgg agtgtcatgc gccgtttcaa ttcatcggga
     7741 tcataatcgg caaccgagct gccgtcgtca accttgcctt gtcgcgtgac cgccccgcta
     7801 tcgtataacg ccgcgtctat cagcgatgtt ttacctgcgc ccacatggga aatcaacgct
     7861 acattgcgaa tgtgttccgc agtatagacc ttcatcttca aatgtcacgg catgaagacc
     7921 catctccgtt tatgggaggg gaggaaatgc cgcttctcct ttctatcaaa tgagccgaag
     7981 ccctctgcac actcttgagg tgtttgtaac tcgcacctgc atgaatcttc gttccatcga
     8041 gggtgcgtag atcaaagtag ccgctgcttc ttctcccaaa cacaaagcag aacgtcccct
     8101 gatactgcac gcaatcaaac aagcgaaagc catggacata cttcggcgcg gtgttccgct
     8161 gcctcttgcc tccttttctg atcgtggctt tgtgcagttg gcggttgttt ctcctcacat
     8221 atttgatcaa ataccaggtg ccatctgatc ctaccagagg atgctggcta atacaacgag
     8281 catcgatcag atgtgacttc tcaagattgt tcgtgatgcg cgtctgtttc gtgatatacc
     8341 catacgtcag atgcacgttt gggaacatcg ctttggcttg ttcatagatg cgccagcgga
     8401 tcatgcccat gtgtgtcgca tcacgccacc ccctggactt ccgctctatc ttgtgctcct
     8461 ggcgcgttcg atgaatgtga tcatggcacg tctcacagag cgtgatgaga ttgtcagggc
     8521 tgtcaccccc cgtc
//
LOCUS       DASZAK010000667         3311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_51696, whole genome shotgun sequence.
ACCESSION   DASZAK010000667 DASZAK010000000
VERSION     DASZAK010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51696"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1387)
                     /locus_tag="VGT44_11745"
     CDS             complement(<1..1387)
                     /locus_tag="VGT44_11745"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV2581514.1"
                     /translation="MSQKPKIRLGAIPKSTMRLLRNTPTYFFLIWRWTWWVYALAWIE
                     FNNKHPWFLQVILGITLIQTLIVTLYSPIFKLLLPGKPTTEARPAGATGRSRSWRRWL
                     RRRARVLGAGDELEALPPLVRSTNVYKNIAIYGLDVVICGLTMYFTAIFSVPPFGDGS
                     PFYRYGFSTIFAAAFVFRYRGGLAAALGYEAFILLGVFIHPPLSVPHYLLQVQDLLGS
                     IFDAPLIAIFVAYMSTLLNSYTSNKRLLQDSGRRQKAMTRLSDRLLQGTSDRQQFLRQ
                     YLEEIRKGGHFARLVVALINQDEIPTSPPSIETFLESGITGATTSDDSKLIALQVARD
                     DKKYSSFEPISGDAYEEGEHLARLYLPLKRMQGDQVSMVIGGERVSAAPFEERQERFL
                     KIVGVQLVIALENIRLTELAAELAAVAERGRIAREMHDGVAQLIYMLSLNTETCVALV
                     QRLSDTLDEEEV"
     gene            1652..2989
                     /locus_tag="VGT44_11750"
     CDS             1652..2989
                     /locus_tag="VGT44_11750"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2581515.1"
                     /translation="MTSTSSNTPASNDDIPASEMPAAPKARVQTFRALRYRNFRFLWI
                     SLIVSSVGTWMQIIAQSLLVLLITHGSALALGIVSLAQASSFFLFALVGGSVADRVDK
                     RRFLLLTQSLSMLLAFVLGTLTITGVIQVWMIVLLAFASGTVLSFDQPTRSSFIPMLV
                     PRQDLMNAISLQSIVFNGSAVLGPTLAGVTIGLLAYAGQAVGLTSKLVGYAGNFYLNG
                     VSYMGVLVVLSLIQVPKDAAEQATEKRGPMLQSIRMALGAVRRDAVLPWVLSGYGALL
                     FFGPSSALILPIFATQILHLSPFQLGLLFSASGLGVITGSLIVASLGDFQYKGWLLLI
                     SLLFWTGALVIFALSRVLWLSLLALLIFGVAQNGVGATVITLAQTRVPPQMRGRVMSL
                     NTLLIMGVRPLGDFPASGLIALAGGPLTVIICAAIVGSYSLYLLLGRSIIRSL"
     gene            complement(3070..>3311)
                     /locus_tag="VGT44_11755"
     CDS             complement(3070..>3311)
                     /locus_tag="VGT44_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581516.1"
                     /translation="PGFWFRCAGVVAILAWIAIRIIGTVPINSATLTWQPGAPPKNWK
                     ALVNHAERFHIVGVWAAVLAFAFFLTAMALKLAVH"
BASE COUNT          607 a          961 c         1005 g          738 t
ORIGIN      
        1 ggacctcctc ctcgtcgagc gtgtcggaga gacgttggac gagcgctacg caggtttcgg
       61 tattcaggct gagcatgtag atcagttgcg caacgccgtc gtgcatttcg cgcgcgatgc
      121 gcccgcgttc ggcgacggcg gccagttcgg cggccagctc ggtcaggcgg atgttttcca
      181 gtgcgatgac cagttgcacg ccgacgatct tcaaaaagcg ttcctgccgc tcttcaaagg
      241 gcgcggcgct cacccgctcg ccgccaatca ccatagagac ctggtcgccc tgcattcttt
      301 taagcggcag gtagaggcgc gccaggtgtt ccccctcttc atatgcatcc ccactgatgg
      361 gttcaaacga gctatatttt ttgtcgtcgc gggcaacttg tagcgcgatg agtttgctgt
      421 catcagaggt ggttgcgccg gtgatgccgc tttcgaggaa tgtctcgatg gatggcggtg
      481 atgtggggat ttcgtcctgg ttgatgagcg cgacgaccag gcgagcaaaa tggccgccct
      541 tgcgaatctc ttcgaggtat tgccgcagaa attgctggcg gtcgctcgtg ccttgcagca
      601 ggcggtcgct caggcgcgtc atggctttct gcctgcgccc actatcttgc agcagtcgtt
      661 tgttgctcgt gtagctgttg agcagcgtgg acatgtaggc aacaaagatg gcaatgagcg
      721 gcgcgtcgaa gatcgatccg agcaggtcct gcacttgcag caaataatgc gggacagaaa
      781 gcggcgggtg aatgaagaca cccagtagga tgaacgcttc gtagccgaga gcggcggcca
      841 atcccccgcg atagcgaaag acgaaagcag ccgcgaaaat agttgagaag ccgtagcgat
      901 agaaaggcga accatcaccg aatggcggca cagaaaaaat cgcggtgaaa tacatagtca
      961 gtccgcagat gaccacgtcc agtccgtaga tcgcgatatt cttatagaca ttggtcgagc
     1021 gcaccagggg aggcaatgcc tccagttcat ctccagctcc taatacgcgc gcccgcctgc
     1081 gcagccagcg ccgccacgag cgggatcgtc ctgtcgctcc cgctggtctc gcttcagtcg
     1141 ttggctttcc cggcagcagc agcttgaaga tgggcgaata gagtgtgacg atcaaggtct
     1201 ggatgagcgt aatgcccaga atgacctgca ggaaccaggg atgtttgttg ttgaactcga
     1261 tccaggccag cgcgtagacc caccatgtcc agcgccagat caggaaaaag taggttgggg
     1321 tgttgcgcag caggcgcatg gtcgatttag gaatagctcc cagccgtatt ttcggtttct
     1381 ggctcatggt gtcacgcgcg ctttctgcat gcctttctaa tggatgcgcc tggcttcatt
     1441 gatgccagta tgactggttt tggctctgct gtcaattgca tcgcagtggc gatagatcat
     1501 ataatgagcc gggacacaaa tttcttatat tcagcagtat gtaccagccg ctggaaaaag
     1561 tgcttcgagt cggccaggcg tttacctacg agtatgattt cggctctacg acggatctta
     1621 aacttaaaat ggtggcgaag aaataggata gatgactagc acttcttcaa acacccctgc
     1681 gtcgaacgac gatattccgg catccgagat gcccgccgcg ccgaaagcgc gcgtccagac
     1741 cttccgcgcg ctgcgctatc gcaacttccg cttcttgtgg atcagcctga tcgtttcgtc
     1801 ggtcggcacg tggatgcaga ttatcgcgca gagcctgctg gtgctgctta tcacgcacgg
     1861 ctcggcgctg gcgctcggaa tcgtttcgtt ggcgcaggcg tcgtcgttct tcctgtttgc
     1921 tctcgtcggc ggcagcgtgg ccgaccgcgt ggataaacgc cgcttcttat tgctcacgca
     1981 gagcctgtcg atgttgctgg ccttcgttct cggcacgctg actattaccg gcgtgatcca
     2041 ggtctggatg atcgtgctgc tggccttcgc ctctggcacg gtgctgagct tcgatcagcc
     2101 gacgcgctca tcgtttatac cgatgctggt tccccggcag gacctgatga acgccatttc
     2161 gctgcaatcg atcgtcttca acggctccgc tgtgctaggt cccacgctgg cgggcgtcac
     2221 catcggcttg ctggcatacg cgggccaggc ggtcggcctg acgagcaagc tggtcggtta
     2281 cgcgggcaac ttctacctga acggcgtcag ctacatgggc gtgctggtgg tgctttccct
     2341 gatacaggtg ccgaaagatg ccgcagagca ggcgacggag aagcgcggcc ccatgctgca
     2401 atcgatccgc atggcgctgg gcgcggtgcg gcgtgacgcg gtcttgccct gggtgctttc
     2461 cggctatggc gcgctgctct tcttcggccc gtcctcggcg ctcattctcc ccatcttcgc
     2521 cacgcagata ctgcatctct cgccattcca gcttggcctg ctcttctccg cctccggtct
     2581 gggcgtcatc accggttcgt tgatcgtcgc ctcgctgggg gatttccagt acaagggctg
     2641 gctgctgttg atctcgctgc tgttctggac cggcgcgctg gtaatcttcg cgctcagccg
     2701 cgtcctctgg ctctcgctgc tggcactcct gatcttcggc gtagcgcaga acggggtggg
     2761 ggcgaccgtc atcacactgg cgcagacgcg cgtcccgccc cagatgcgcg ggcgcgtcat
     2821 gagcctcaat accctgttga ttatgggggt gcgcccgctc ggcgatttcc cggcgagcgg
     2881 gctgattgcc ctggcaggcg gtccattgac agtcatcatc tgcgctgcta tcgtcggcag
     2941 ctactcgttg tacctgctgc tcggcaggtc gattatccgg tcgctatgac ggaactcctc
     3001 ttgtgctgcc tccatccgtg gtggggacac gatgtcgcat ctgacgagac gggtgagctg
     3061 tctcacaggt caatgaacgg ccagcttgag tgccatagcc gtcaagaaga aggcaaatgc
     3121 caggaccgcc gcccagacac caacaatatg aaagcgctcc gcgtggttga ccaacgcctt
     3181 ccaattcttt ggtggcgcgc cgggctgcca ggtgagtgtg gcactgttga tagggacggt
     3241 accgatgatc ctgatcgcga tccaggcgag tatggcaacc acgcccgcgc accggaacca
     3301 aaagccgggc g
//
LOCUS       DASZAK010000668         3750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_478728, whole genome shotgun sequence.
ACCESSION   DASZAK010000668 DASZAK010000000
VERSION     DASZAK010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478728"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..804
                     /locus_tag="VGT44_11760"
     CDS             196..804
                     /locus_tag="VGT44_11760"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084140945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV2581517.1"
                     /translation="MVAPALPATNLHWSSASSPRGTCVVMATERGVCWAGTPGTPMED
                     GLAWVRRKLNFGQVIEGENIAPLQEAMSYMRRYLAGEPVQFACQLDLHGTPFQLAVWK
                     ALYRIPYGETRTYAEIAEEIGHPTAVRAVGAANGANPIAIIVPCHRVIGSNGALTGYG
                     GGLPTKAWLLALERNASRDTTANITGDNYYPPRGTSLPARAR"
     gene            complement(746..1120)
                     /locus_tag="VGT44_11765"
     CDS             complement(746..1120)
                     /locus_tag="VGT44_11765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581518.1"
                     /translation="MDDTTSTSNTSVTATQPAANTVDDSAHRQAYGTAKTENLRDSGL
                     WRILLPGFVLVASVILLAFPLIILTPLFLHSLDPLAAANQLNRPETWIWIVMFLVEVG
                     VIAVIVRGLAKTFLVGDSNYRQ"
     gene            1203..1769
                     /locus_tag="VGT44_11770"
     CDS             1203..1769
                     /locus_tag="VGT44_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581519.1"
                     /translation="MAERIPPASAPTNTISWWSWRTGMSPIRAVLTGLLLLLLAVFLI
                     LVGGETLVSGFIDSSSLHHTSNAPGSLILFVGGLLLLPYTLYLTFGGWHDLRGQRRMV
                     VGKVIALRTTARNVLRQGRPVRPGLASGIARAWYGMALQPDEPHEPVMIFRLSEDHYR
                     DLREGEFVQVSYTLHLHHVLSLRPTDAN"
     gene            1759..2493
                     /locus_tag="VGT44_11775"
     CDS             1759..2493
                     /locus_tag="VGT44_11775"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV2581520.1"
                     /translation="MPTDARNGAIQRVKRGLSPLMDALYPPHCVGCQRTGAIICADCL
                     AQMAPLALPVCPCCGIPVPQAALCQSCQRDPPRITALRSASKYQEPLRGCIRALKYEG
                     NTRLAEPLGLVLAQAFRRYGLSADALLPVPLHPERHAKRGYNHATLLAQVCARHLALP
                     CYEQMLLRHRDTLSQVGLTPRERQQNVQGAFVCSPAFTRGALTGRTLLLIDDVCTSGA
                     TLEACAAPLFAAGAAAVIGLALARPV"
     gene            2792..>3750
                     /locus_tag="VGT44_11780"
     CDS             2792..>3750
                     /locus_tag="VGT44_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2581521.1"
                     /translation="MASSGKWIRSSFIWLVLAAVIVVIIILFFRTSTNSNQVDVSSLL
                     NDLQTDISHNQLDTLSVGIDTLTLTRGQSTTNEVANINSTFDITQVLKDKSIDYTNSK
                     VVDLVYEPPSALGAWLAVLGGLLPFIFIGGLLIFMMRQAQGSNNQALSFGKSRARMFM
                     GNKQTITFADVAGVEEAKQELQEIVEFLKFPDKFAALGARIPKGLLLVGPPGTGKTLI
                     SRAVAGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRNSPCIVFVDEIDAVGRQ
                     RGAGLGGSHDEREQTLNQILVEMDGFDTNTNVIVIAATNRPDVL"
BASE COUNT          757 a         1134 c         1099 g          760 t
ORIGIN      
        1 gcgggggcag ggcggtgaag ggaggggacg cttgcgtcgc ccgcgtacct ggtggcgcag
       61 ccgaatggca ttgacaacgg actacgcggg cgacgcaagc gtcccctccc ttcaccgccc
      121 tgcccccgcc cctacagaac cgattgtccg ttagagttgg aagattctat caggaccttt
      181 ttgcaggaga ttgacatggt tgcgccagcg ttaccggcaa caaacctcca ttggagcagc
      241 gcgtccagcc cgcgtgggac atgcgtggtg atggctaccg aacgcggcgt atgctgggcg
      301 ggaacacccg gcacaccgat ggaagacggg ttggcgtggg tacgtcgcaa gctgaacttc
      361 gggcaggtga tcgagggaga gaacatagcg cctttgcagg aggccatgag ttatatgcgg
      421 cgctacctgg caggcgaacc tgttcagttt gcctgccagc ttgacctgca tggcacaccc
      481 ttccagctag ccgtgtggaa ggcgctctat cgcattccct acggggaaac acgaacatac
      541 gcggagattg cggaggaaat cggacatcct actgctgtgc gcgccgtcgg cgcagccaat
      601 ggagctaatc ccatcgccat tatcgttccc tgccaccgcg taatcggcag caacggcgcg
      661 ctcaccggct acggcggtgg gttgccgacg aaagcgtggc tattggcatt agagcgaaac
      721 gccagccggg atacaaccgc aaatattact ggcgataatt actatccccc acgaggaacg
      781 tctttgccag cccgcgcacg ataacggcga tcacgccgac ttccaccagg aacataacga
      841 tccagatcca cgtctccggc ctgttcaact ggttggcggc agccagcggg tcgagggaat
      901 gcagaaagag cggggtcaaa ataatgagtg ggaatgccag caagatgacg ctggcaacca
      961 gcacaaaacc cggcaacagg atgcgccaga ggccagagtc gcgcaggttc tcggttttcg
     1021 cggtgccgta agcttgccgg tgggccgaat cgtcaactgt gttcgctgct ggttgggtcg
     1081 cggtaacact tgtattcgag gttgatgtgg tatcatccat agaaagctct cctctgattg
     1141 agtcctgggc tattgatggt tccagtatat cacagtttct cgtggaaagg agactgcgac
     1201 cggtggcaga gaggattccg cccgcttctg ctccaaccaa caccatttcc tggtggagct
     1261 ggcgaactgg catgtcgccc attcgtgctg tgctgaccgg cctgctcttg ctgctgcttg
     1321 ccgtcttcct catactggtg gggggtgaaa cactggtctc cggcttcatc gatagttcat
     1381 ccctgcatca caccagcaat gctccaggct cactgatcct gtttgtaggc ggtctgctcc
     1441 tgctgcccta tacgctctac ctgaccttcg gcgggtggca tgacctgcgc ggccagaggc
     1501 gcatggtggt gggcaaggtc atcgcgctgc gcaccaccgc cagaaacgta ctccggcagg
     1561 ggcgtcccgt ccggcccggc ctggctagcg gcatcgcgcg cgcctggtac ggcatggcct
     1621 tgcaaccaga cgagccacat gaaccggtca tgatttttcg cctctccgag gatcactatc
     1681 gtgatctgcg tgagggcgaa ttcgtgcagg tcagctacac ccttcacctg catcatgtgc
     1741 tatcgctgag gccaaccgat gccaactgac gctcgaaacg gcgccataca acgcgtcaaa
     1801 cgcggcctct cgccacttat ggatgccctc tatcccccac attgcgtggg ttgccagcgc
     1861 accggcgcta tcatctgcgc tgactgtctc gcgcaaatgg ctccactcgc cttaccggtc
     1921 tgtccgtgct gcggcattcc ggttcctcag gcagcgctgt gccagtcttg ccagcgcgat
     1981 ccaccgcgca ttactgcgct ccgctccgct agcaagtacc aggaaccact gcgcggctgc
     2041 attcgcgcgc tgaaatacga gggcaatacc cggctggccg agccattggg actggtgctg
     2101 gcccaggcgt tccgccgcta cggactctca gccgatgcgc tgctgccggt gccgctgcac
     2161 cctgaacgcc atgcaaaacg tggctacaat catgcgacgc tgctggcaca ggtctgcgcg
     2221 cggcatctcg ccctcccctg ctacgaacag atgctgctgc ggcatcgcga cacgctctcc
     2281 caggtggggt taacacccag ggaacgccaa caaaacgtgc agggcgcgtt tgtctgttcg
     2341 cctgccttca cacggggcgc gctaaccggc agaacgctgt tgcttatcga cgatgtctgc
     2401 acatcaggcg caacgctgga ggcatgcgcc gcgccgctct tcgccgccgg cgcagcggca
     2461 gtcattggat tggcgctggc cagaccggtt tagttgccag ggcgatgctg gccatcgcca
     2521 catagctaca gcacgatacc cctatcccct acgaatgtgc gcaagtttat gctataatac
     2581 ccctcgaaag tgtaacaaaa ctggcacatc tatgctgttg agccgtaata tagatatgat
     2641 tatgcgtcgt gtcatggtaa gggcgcaccc tggcggtcgc cctgcgaggt tcgccaactg
     2701 cgtcgtgtca tggtaagggc gcaccctggc ggtcgccctg cggtaattcg ccaacagtaa
     2761 cattgcttga tgataaaaga gaggtctcct catggcgagt agtgggaaat ggattcgcag
     2821 tagttttata tggctggtac tggcagccgt cattgttgtt atcatcattt tgtttttccg
     2881 cacgtcaacg aactcgaatc aggtggatgt ctcctcgctg ctcaacgatc tgcagaccga
     2941 catcagccat aaccagctag atacgctctc cgttggcatc gatacattaa cgctcacacg
     3001 cgggcaaagt acgacgaatg aggttgccaa catcaacagc accttcgata tcacccaggt
     3061 gctaaaagac aaaagcattg actatacgaa cagcaaggta gttgacctgg tgtacgaacc
     3121 gccaagcgcg ttgggtgcgt ggctcgctgt gcttggcggc ctgctgccgt tcatcttcat
     3181 cggcggtctg ctgatcttta tgatgcgaca ggcgcagggc agcaacaacc aggcgctctc
     3241 attcggcaag agccgcgcgc gtatgttcat gggcaacaaa cagaccatca ccttcgcgga
     3301 cgtagcgggc gttgaagagg ccaaacagga actgcaagag atcgttgagt ttctcaaatt
     3361 ccccgataaa ttcgccgcac taggcgcgcg tattcccaaa ggtctgctac tcgtcggccc
     3421 gccgggtacg ggcaagacac tcatttcgcg cgccgtcgcg ggcgaggccg gcgtgccatt
     3481 cttcagcatc agcggctccg agttcgtcga aatgttcgtg ggtgtgggcg cgagtcgcgt
     3541 acgcgacctc ttcgaccagg ccaagcgcaa cagcccctgc atcgtgtttg tggatgagat
     3601 cgacgcggtg ggccgccagc gcggggccgg tctgggtggc tcgcacgacg aacgcgagca
     3661 gacgctcaac cagatcctgg tcgagatgga tggcttcgac accaacacca acgtgatcgt
     3721 gatcgccgcg accaaccgcc ccgacgtgct
//
LOCUS       DASZAK010000669         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_585755, whole genome shotgun sequence.
ACCESSION   DASZAK010000669 DASZAK010000000
VERSION     DASZAK010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585755"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VGT44_11785"
     CDS             complement(<1..1433)
                     /locus_tag="VGT44_11785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2581522.1"
                     /translation="MALTSFLYLPPLARSVDYRAYLAPVAGVTFLRRFLERLHISKNE
                     LRVVVLCGDEPKIDVVTEIGRVAGTEVVPTFRSRPIEAFAEAAEKTCSEQAAFFSMEL
                     SLAPDDLLVRVLRHHLACGNDYTAVTGLPEECTPAVYAKSLLLSLLALLQSLPFFPPY
                     PRLAMDMLLRLPEKPFPVASTPFNGAREFGPDTEGLPNSARIATPEDMEVVQDILSGR
                     VSGGLARPLEKLYQWKQIREERREQARRVLRSRIGYLAPPGKLTRPEKRPRARVLYIS
                     NPSAFSGGEASLCQLAAHVDRRRFDLFALLALRGHFARRLEQLGVEVVCPDRDFVDSD
                     IDNFLWALATLGDIAPDVVHLNAIVGMHMVYAAIVLGIPIVQHLRVVDPDPVLVKQAS
                     AADAVITVSDFVRKEVLKCEIAPQRVRVIHSGVDPEYFRPDAFDKLEMRQKYGLPLTA
                     KIMLMVARFAPNKRHDLILRAASLLRSS"
     gene            complement(1494..>2277)
                     /locus_tag="VGT44_11790"
     CDS             complement(1494..>2277)
                     /locus_tag="VGT44_11790"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2581523.1"
                     /translation="IIAGRSFSETDTDVVIISQRTAREYWKGGNPVGDAFWLEGEERP
                     RQVIGIVGDTTATGLGEAPHLQIYLPYGHAYRGKIPALSTFLLARCGTGTTDCPSVLM
                     QQITNAGGGSSVDRFAGLEDLVQASIAPSKAQAVLLGFYALLGLALAWLGVLALVSYL
                     AAVRTYEMGVRMALGAQALDVVFALSSEGLWCAGVGLVVGLGASFAAVRVLASLLFGV
                     KPLDPASFALAAFLLSSGVLLASVIPAFRVARREPRRLLQSC"
BASE COUNT          546 a          700 c          618 g          413 t
ORIGIN      
        1 gaggaccgga gaaggcttgc tgcgcgaaga atcaagtcgt ggcgcttgtt cggcgcaaag
       61 cgagccacca tgagcatgat cttcgctgtg agaggaagtc cgtatttttg gcgcatctcc
      121 agcttatcga acgcatccgg acgaaagtac tcgggatcca ccccgctgtg gattacgcgg
      181 accctctgag gggcgatctc gcatttcagc acctccttcc ggacgaaatc ggagacagta
      241 ataactgcgt cggcggcact tgcttgcttc acgagaaccg gatccgggtc cacgacccgc
      301 aaatgctgta cgatgggaat tccaagaacg attgcggcat ataccatatg catgccgacg
      361 atggcgttta ggtgaaccac gtccggagct atgtcaccca gtgtcgccag cgcccacaga
      421 aaattgtcta tatcactgtc cacaaaatcg cggtcgggac acacaacctc cacgcccaac
      481 tgctccaaac ggcgggcgaa atgtcctcgg agcgccaata aagcaaacag atcgaagcga
      541 cgccgatcta catgggccgc caactggcac aggcttgcct cgccgcccga gaatgccgat
      601 gggtttgaaa tatataggac gcgcgcccga ggccgtttct ccgggcgagt cagttttcca
      661 ggaggggcaa gatagcctat ccgtgaccga agcacgcgac gcgcctgctc tcttcgttcc
      721 tccctgattt gcttccactg atacaacttt tccaagggtc gcgccaaccc acctgaaacc
      781 ctgccgctca ggatatcttg caccacttcc atatcttcag gtgtggcgat ccgcgcggag
      841 ttgggcagac cttccgtatc tggtccgaac tctctggctc cattaaaggg cgtggaagcc
      901 acaggaaagg gcttctcggg caagcgcaag agcatatcca ttgccaatct cggatatggt
      961 ggaaagaacg gaaggctctg caaaagggcc aagagggaaa gcaggagaga ttttgcgtat
     1021 acggcggggg tgcattcttc aggtaatccg gtgaccgcgg tgtagtcgtt accgcacgcg
     1081 agatgatggc gcaacacccg cacgaggagg tcgtcaggcg caagggatag ttccatcgag
     1141 aagaaagccg cctgctcact gcaggttttc tccgccgcct cagcaaaggc ttctattggt
     1201 ctgctccgaa aggtcggaac gacctcggtc ccagcaaccc ggccaatttc cgtgacgacg
     1261 tcgatttttg gctcgtcgcc acacaacact acaaccctca actcattttt gcttatatgt
     1321 aacctttcaa ggaacctccg taggaaggtt actcccgcga ctggggcaag gtaggcgcgg
     1381 tagtccacag aacgagcaag cggcggcagg tagagaaatg atgtcagcgc catagaccac
     1441 aagtgccgaa gagtccttcg tctggccctt cccaaacagc gccacctctg ctgttagcag
     1501 gattgaagca aacgccgagg ttcgcgccgc gccacccgga aggccgggat aacagatgcg
     1561 aggagaacgc cggagctcaa cagaaaagca gctagcgcaa aactggcggg atccagtggc
     1621 tttacgccga aaaggaggct ggccaagact cgcaccgccg cgaatgacgc gcccaatcca
     1681 actaccagcc ccaccccagc gcaccagagg ccctcgctcg aaagggcgaa caccacatca
     1741 agcgcctgcg cgcccaaagc catgcgaact cccatttcat aagtgcgcac cgcagcaaga
     1801 taagacacca gcgctaaaac gccgagccag gcaagggcaa ggcccagaag agcgtaaaaa
     1861 cccagcagca ctgcctgcgc cttcgagggc gcaatcgagg cctgcaccaa gtcttccaga
     1921 ccagcaaaac ggtcaaccga gctgccaccg cctgcgttcg taatctgttg catcagcacc
     1981 gaagggcaat cggtcgtccc cgttccgcac cgcgccagga gaaaagtgga taaagcgggg
     2041 atcttgcccc gatacgcatg gccgtatggc aggtagatct ggaggtgggg cgcttcaccc
     2101 agccctgtcg ccgtcgtgtc ccctacaatg ccgatcacct gtcgcggccg ctcttcgccc
     2161 tcaagccaaa acgcatctcc aacaggattc cctcctttcc aatactctct ggctgtcctt
     2221 tggctgatga ttacaacatc cgtgtccgtc tccgaaaacg accggcccgc aatgatc
//
LOCUS       DASZAK010000670         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_515224, whole genome shotgun sequence.
ACCESSION   DASZAK010000670 DASZAK010000000
VERSION     DASZAK010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515224"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VGT44_11795"
     CDS             complement(<1..281)
                     /locus_tag="VGT44_11795"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2581524.1"
                     /translation="MQLEQALQIYYLMRLTRAIEDRTRTLFLQGRIVGGVYTAQGHEA
                     TTVGAAMLLGDGDFIVPQHRDLGMHLTRGVSPHAVMCQWLARGNSPTLGR"
     gene            612..>2255
                     /locus_tag="VGT44_11800"
     CDS             612..>2255
                     /locus_tag="VGT44_11800"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2581525.1"
                     /translation="MGMTKDGLSYWQAETEGIELLDTTFGDLLDARAEEIPAQEAVVY
                     SCYPEFGGALDIRWSYAEFRERVNAVARGLMALGLQKGEHVGVWAANVPEWVLLEFAA
                     AKAGLVLVTINPVYRAAELEYVLKQGDVAALFFMARVRDHDCLATIRSMVTPSVYNGE
                     VSSERLPALRYACLVGAPPADLLQQEGWRPALFREMVAGGVAISAEALKERQASVKPG
                     DPALILYTSGTTGFPKGAILSHSAVINDLMLAFHRSGDAFQPGDRYCLPMPLFHIAGS
                     GTAVGVVVKRMTLHPLIAFDVLKTLQIIKQEGCHTIFAVPTMLIAMLQHPEFETYKPA
                     TLKVVTSGGAPVPVALMEQVKARMGSDVAIVFGQTESTGGVTITHLDDSFERKSATVG
                     TPYAHVDIKITDPATGQVVPVGERGELCCRGFLVMSGYYDMPEKTAETIDSDGWLHTG
                     DLATIDAQGYANIVGRLKEMVIRGGENIFPREIEDLLIRHPKVSDAAVLGVPDSFFGE
                     ELLAVVLPREGAELTEQELRDYLKERVNHQKIPRYIQFTQ"
BASE COUNT          461 a          685 c          689 g          420 t
ORIGIN      
        1 cgtcccagcg tgggggagtt gccgcgcgcc agccactggc acatcaccgc gtgtggagag
       61 acgccgcgtg tgagatgcat ccccaggtcg cggtgctggg gaacgatgaa atcgccgtcg
      121 cccagcaaca ttgccgctcc gacggtggtg gcctcgtgtc cctgcgctgt atagacgccg
      181 ccgacaatgc gcccctgcaa gaagagggtg cgtgtgcggt cttcgatggc acgggtaaga
      241 cgcatgaggt agtagatctg aagcgcctgc tccagttgca tacgtagcct tcctttactg
      301 atgcgcgaga atttcaagca tcattgctca gcaattcgac acattcaggg tgaggcgttc
      361 tcagaggaaa gggatattcg atccgtcacg gtcacgtcag gcctttgcat tccagcctgc
      421 ccaccctgct ctccccagca atggtagaga ggttatctgc gttcatgcta gcacctggaa
      481 agtcggggtg tcaagttgaa tgtgatatca tgatgatgca cattgacaag ataaagcggc
      541 caccagagca gggtagataa gggcgaccgc aagggtcgct caatattttt catcatacag
      601 gagagttata tatgggcatg accaaggacg gcctgtccta ctggcaggcg gaaactgaag
      661 gcatcgagct gcttgatacg acgttcggcg acctgttgga cgcgcgcgcg gaggaaattc
      721 cagcacagga agcggtggtg tactcgtgct acccggagtt cggcggcgcg ctcgatatcc
      781 gctggagcta cgcagagttt cgcgagcggg tcaacgcggt ggcgcgcggg ctgatggcgc
      841 tgggcctgca gaaaggcgag catgtcggcg tgtgggccgc caacgtgcct gagtgggtgc
      901 tgctggaatt cgccgccgcg aaagcggggc tggtgctggt cacaatcaac cccgtctacc
      961 gcgcggcgga actggaatac gtcctcaagc agggcgacgt ggcggcgctc ttcttcatgg
     1021 cacgggtacg cgaccatgat tgcctggcaa ccatccgctc gatggtcacg cccagcgtgt
     1081 ataacggtga ggtcagcagc gagcgcctgc ccgcgctgcg ctacgcctgc ctggtgggcg
     1141 cgccacccgc cgacctgtta cagcaggaag gatggcgacc tgcgcttttc cgcgaaatgg
     1201 tagcgggcgg cgtcgccatc agcgcggaag cattgaagga acggcaggct tcggtgaaac
     1261 cgggcgatcc agccttgatc ctgtacacat caggcacgac cggcttcccg aaaggcgcca
     1321 tcctctcgca cagcgccgtc attaatgacc tgatgcttgc ttttcatcgt tccggcgacg
     1381 cctttcagcc aggcgaccgc tactgcctgc cgatgcccct gttccacata gcaggttctg
     1441 gcacagccgt tggcgtcgtc gtcaagcgca tgacgctgca cccactgatc gccttcgatg
     1501 tcctcaagac actccagatc atcaagcagg aaggctgcca tactatcttc gccgtcccga
     1561 ccatgctgat cgctatgctc cagcatccag agttcgagac ctacaagcct gcaacgctga
     1621 aagtcgtcac gagcggcggc gcgccggttc ccgtcgcctt gatggaacag gtcaaggcgc
     1681 gtatggggtc ggatgtcgcc atcgtcttcg gccagaccga atcgaccggc ggcgtgacca
     1741 tcactcacct cgacgactcc ttcgagcgca aatccgcgac ggttggaacc ccctacgcgc
     1801 acgtcgatat caagataacc gatcccgcga cgggccaggt tgtcccggtc ggcgagcgcg
     1861 gcgagttgtg ctgccgtggc ttcctggtca tgtccggcta ctacgacatg ccggagaaga
     1921 ccgccgagac catcgatagc gacggctggc tgcataccgg cgacctggcc accatagacg
     1981 cgcagggcta cgccaacatc gtcgggcgcc tgaaggagat ggtcattcgc ggcggcgaaa
     2041 acatcttccc acgcgagatc gaagacctgc tcattcgcca tcccaaagta tccgacgcag
     2101 ccgtgctggg cgtgcctgac agcttcttcg gcgaagaact actggcagta gtgctgccca
     2161 gagaaggcgc tgagctgacc gagcaagaac tgcgcgacta cctgaaagag cgcgtcaacc
     2221 accagaaaat cccgcgctac atccagttca cgcaa
//
LOCUS       DASZAK010000671         3349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_515230, whole genome shotgun sequence.
ACCESSION   DASZAK010000671 DASZAK010000000
VERSION     DASZAK010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515230"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VGT44_11805"
     CDS             complement(<1..736)
                     /locus_tag="VGT44_11805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2581526.1"
                     /translation="MTSTSIFRPELFNGQVFLITGGGTGIGRAVARELSSLGASVAIC
                     SRSMAHLEDTYAEIEATGGQVFARTCDIRSEEQVNAQIQAVLEYFGRLDGLVNNAGGQ
                     FFSPAENITSRGWQAVIETNLTGTFYMSQAAMRHWMSRHGGVIVSVVFDMWRGFPHLA
                     HSSAARAGVVNLTQTLALEWAQYGIRINVVAPGLVASSGLSTYPDEVREGLQASMQVH
                     PARRMATESEVAAAILFLLSPAAAYIS"
     gene            complement(740..1051)
                     /locus_tag="VGT44_11810"
     CDS             complement(740..1051)
                     /locus_tag="VGT44_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581527.1"
                     /translation="MKKQLVHLAHARSGDKGDKANIAIFAPDRATYDILLREVTGERV
                     RAHFVGIITGNVERFEVPNVLALNFVLHGALNGGASRSLRSDSLGKSLSSALLRMEIE
                     V"
     gene            complement(1048..2412)
                     /locus_tag="VGT44_11815"
     CDS             complement(1048..2412)
                     /locus_tag="VGT44_11815"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEV2581528.1"
                     /translation="MKSIRIAAGLGFYGDSWRPVRASIEHGNVQYVCSDHLAELTLAI
                     LHKNRQRDPNLGYTQDLVPMLSDLLPLAVQYGVKFVLNAGGLNPLAAREALLAVLRKL
                     GLKLKVSVVLGDAVLERLDELQAAGASLAHMDTGADIAEVSERLLFASAYLGARPLVE
                     ALDAGADIVLTGRVADAALFLAPLVHELGWRWDGWDKLAQGIVIGHLLECSAQATGGN
                     FGGDWRSIPDLAHIGYPIAEVWENGEAVISKAPGTGGRVSFDTLREQLLYEVHDPAHY
                     LTPDVDVNMTTLHLEEIAPDQVRVTGASGRPAPKTLKVVAGYEDGVMGQAGIGYAWPD
                     ALAKAQLAAQIITQQMAEMGLKSEETHVEYQGFNSIHGPLADPSHADDLNEVYLRIAV
                     RCADRKEAAKLGRLVPPLGLSGPPFIGGLGGMMEPRGLLGIWPTLVPRAMIEEHIRVS
                     VEEA"
     gene            complement(2417..>3349)
                     /locus_tag="VGT44_11820"
     CDS             complement(2417..>3349)
                     /locus_tag="VGT44_11820"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2581529.1"
                     /translation="ASAIARASAAIATSYFAHLNLCAHNILRNGSERQRQKYLPGLAS
                     TSTIGALALTEPDAGSDAMGIRATARPVDGGYLINGTKVFITNGPIADVMVLYAKTRP
                     EAGSRGITAFIFETTTPGFHVARKLTKVGLHGSPTGELVFEDAFVPEENVLGTVNEGY
                     KVVMGGLDIERAFYSVLGVGTIEEALALSLKYAQEREQFGQPIARFQLIQAKLADMYT
                     DLEAARQVAHRAIWLAQQEGRVSKEAAAAVLFVSRAASRAANEALQIHGGSGYLTDFD
                     VERLWRDARLGEIGAGTNEIRQLLIARELLGLRG"
BASE COUNT          643 a         1065 c         1005 g          636 t
ORIGIN      
        1 cggaaatata agcggcggcg ggtgagagca ggaagagaat ggcggcagcg acttcacttt
       61 cggtagccat gcgccgcgct gggtgcacct gcatggaggc ttgtagccct tccctgacct
      121 cgtcgggata ggttgagagt cctgaagagg caacgagtcc tggcgccaca acgttgatgc
      181 gaatgccata ctgcgcccat tccaatgcca gtgtctgagt gagattgacc acgcccgcac
      241 gcgccgcgct gctgtgtgcc agatggggaa agccgcgcca catgtcgaaa acgacgctga
      301 cgatcactcc accgtgcctg ctcatccaat gacgcatggc cgcctgggac atgtaaaagg
      361 tgccggtcag gttggtttcg atgaccgcct gccatcccct ggaggtgatg ttctccgccg
      421 gagagaagaa ttgccctccg gcgttattga caagcccatc taatcgcccg aagtactcaa
      481 gcactgcctg gatttgagca ttgacctgct cctcgctgcg aatatcgcat gtccgagcaa
      541 acacttgccc accagtggcc tcgatctccg cgtaggtgtc ctccaggtgc gccattgagc
      601 gcgaacagat tgccacactt gctccaagtg aggagagctc acgagcaact gcccgcccaa
      661 tacctgtccc tccaccagta atgaggaaca cctgcccatt gaacaattcc gggcgaaaga
      721 tgctggttga tgtcattatt tatacctcga tctccatccg cagcaaggca gatgaaagcg
      781 atttccccag agaatcgctg cgcagcgagc gcgatgcgcc accgttgagt gcgccgtgta
      841 agacaaagtt caacgcaagc acattgggaa cctcgaaccg ttcgacattg ccggtgatga
      901 tgccgacgaa gtgtgcgcgc acgcgctccc cggtcacttc gcgcagcaat atatcatagg
      961 tcgctctgtc aggggcgaag atggcgatgt ttgctttgtc tcccttatcg cctgagcgag
     1021 cgtgagccag atggaccagt tgcttcttca tgcttcctcc acactgacac ggatgtgctc
     1081 ttcgatcatg gcacgtggaa cgagtgtagg ccatatgccc agcagaccac gcggctccat
     1141 catcccacca agccctccga tgaaaggggg gccgctcaat ccaagaggag ggacgagccg
     1201 accgagtttg gcggcctcct tcctgtcggc acagcggaca gcgatgcgca gatagacctc
     1261 gttcaggtca tcagcatgcg aaggatcggc gagcgggccg tgaatcgagt tgaagccctg
     1321 gtattcgacg tgggtctcct cggacttcag ccccatttcg gccatctgct gcgtgatgat
     1381 ctgcgccgca agctgcgcct tcgccagcgc gtcaggccag gcgtagccga tgccggcctg
     1441 tcccatcacg ccgtcttcgt agcctgcaac gaccttgagc gttttgggtg ctggccgccc
     1501 gctcgcgcct gtgacgcgca cctggtcggg agcgatttcc tctagatgca gtgtcgtcat
     1561 attgacatcg acatcgggcg tcaggtagtg cgcgggatca tgcacctcgt agagcaactg
     1621 ctcgcgcagc gtatcgaagc taacgcgccc acccgtgccg ggcgctttgc tgataaccgc
     1681 ctcgccgttt tcccacacct cggcgatggg gtagccgata tgcgccaggt cgggaataga
     1741 acgccagtcg ccgccgaaat tgccgccagt ggcctgcgcg ctgcactcta gtaggtggcc
     1801 gatgacgatg ccctgcgcca gtttgtccca gccatcccag cgccagccca gttcatgcac
     1861 gagaggcgcc aggaagagcg cggcgtcggc cacacgcccc gtcaggacga tatccgcacc
     1921 cgcgtccagc gcctcgacca ggggacgcgc ccccagatag gcgctggcga agagcagtcg
     1981 ctcactcacc tcggcgatat ccgctccggt atccatgtga gcgagcgacg cgcccgccgc
     2041 ttgcagctca tcgagacgct ccagaacggc atcgcccagc acaacgctga ccttgagctt
     2101 gaggccgagc ttgcgcaaga ccgccagcag cgcctcgcga gccgccagcg ggttcaagcc
     2161 gcccgcgttc agcacaaact tgacgccata ctgcacggcc aggggaagca ggtcggacag
     2221 catcggcacg aggtcctggg tatagcccag gttggggtcg cgctggcggt tcttgtgcag
     2281 aatcgccagc gtcaattcgg ccaggtgatc tgagcagacg tattgcacgt tgccgtgttc
     2341 gatgctggcc ctgacgggtc gccaggaatc cccgtagaaa ccgaggccag cggcgatgcg
     2401 aatagatttc ataactctac cctctcaggc ccagcagttc gcgggcgata agcaactggc
     2461 gaatttcgtt tgtccccgcg ccgatctcgc ccaggcgggc atcgcgccac aggcgctcca
     2521 cgtcgaagtc ggtcaggtag cccgatccgc cgtgaatttg tagtgcctcg ttcgcagccc
     2581 gtgacgctgc gcggctcacg aacagcaccg ccgccgctgc ctctttgctg acgcgccctt
     2641 cctgctgggc cagccagatg gcgcggtgcg cgacctgccg tgccgcctcc aggtcggtgt
     2701 acatatcggc cagcttggcc tggatcaact ggaaacgcgc gatgggctgg ccaaattgct
     2761 ctcgctcctg cgcgtacttg agcgagagag ccaacgcctc ttcgattgtg ccgacgccta
     2821 gcaccgagta gaaggcacgc tcgatatcca gcccgcccat gacaaccttg tacccctcgt
     2881 taaccgtgcc cagcacattc tcctctggca caaaggcgtc ctcgaaaacc agctctccgg
     2941 tgggtgagcc gtgcagacca actttcgtca gcttgcgcgc cacgtggaag ccgggcgtcg
     3001 tcgtctcaaa gatgaaagcg gtaatgcccc ggctgccagc ctctgggcgc gtcttggcat
     3061 acagcaccat cacatcggca atggggccgt tggtgatgaa gactttggtg ccgttgatga
     3121 ggtagcctcc atccaccggg cgtgccgttg cgcgaatgcc catcgcgtcg gaaccggcat
     3181 ctggctcggt cagggccagt gcgccaatcg tgctggtgct ggcaagaccc ggcaggtatt
     3241 tctggcgctg acgctcgctg ccgttgcgca ggatattgtg cgcgcacagg ttcaggtgag
     3301 cgaaatagga ggtggcgatg gcggcactgg cgcgtgcgat ggcgctggc
//
LOCUS       DASZAK010000672         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_123226, whole genome shotgun sequence.
ACCESSION   DASZAK010000672 DASZAK010000000
VERSION     DASZAK010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123226"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..>2703)
                     /locus_tag="VGT44_11825"
     CDS             complement(109..>2703)
                     /locus_tag="VGT44_11825"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2581530.1"
                     /translation="SGAAHFSVQLPALTVQSITSGGTTSGASNTKRLLSIYSYAMDQI
                     AGELSIVQGRLPQQAANNVIEIALIQSTAQALGVHVGSVIGANLPQSIASGGTWTLRV
                     VGIFALKANWEASNTFQAQGGSSGNLYPALASNAAILPAIAPLQVQLVSGGKRAFKAG
                     GDVPFFQLNWSYPFNSSQVTASNAGTLLSLADTAQSGIDNSLSNLPGTFFIPRGSGAL
                     FDILPDYYIRIYVEEIVVSSSLILILGLVLFLVSMMTSALIEQQAATIATLRSRGATL
                     RHVYGAFAAQGIGLGLTALLAGPLLAIVLIGLLVQWLFPASEQSAVGILTGNPLQAVL
                     SVGWYALGAVLIAILVVIVAIRRASAMDMLAFRRESARSMRPSLWRRLRLDLMLSLLV
                     CVGYAAYAYLAQSALSDQPDVKIILGLAALIAPLLLLAAGITLFLRIFPLFLRLGTRL
                     TMRRRGASAALAFAQMERAPRPAARMILLLALGTAFGLFMLTSVSTQQQRVADAAVFH
                     VGADFSGSISPPPQGQSLAQAEAVYQQTAGVTSATLGYQAISVNNNGDFVTNHIVAVD
                     TQTFAQTALWSPLYSDQTLSSLMSLLAAHGADAAAHDVVYAIVDDTTAKSMNLSVGSS
                     FILPTFDNFNTHMIVAAIVHAIPDVYSDSGNYGLLVDYQSYATAYMKDSGGGTLAPNF
                     AWLQTRGDAASLASVRKAYPDLQDLRATLDKYQSDPLYTDLVSLLGAGIAVGLLLALA
                     GTLFSAWLNASGRLTSFAVLRALGMVPRKIAAVLLWELGSICAAALALGIGLYLLLKQ
                     LILPTLTITDFVEGIDSRMPLQVVSPTLLIALALGGVICVCMIALALMARLVSRPSLS
                     QTLRLNED"
BASE COUNT          572 a          811 c          849 g          471 t
ORIGIN      
        1 ccgttgacat cgcgattcgg ttgctccctc aggacggcgg gcgacgcaag cgtcccctcc
       61 ccacaccgcg tcctcccccg cccctacgga tgattaatac atttatgttt agtcctcgtt
      121 cagccgtagc gtctggctca gcgatgggcg cgagacgagc cgcgccataa gcgccagggc
      181 gatcatgcag acgcagatga ccccacccag cgcgagggcg atcaagagcg tgggcgacac
      241 gacctgcaat ggcattcggc tgtcgatgcc ttcaacgaaa tcggtaatgg tgagagtggg
      301 caggattaac tgcttcagca gcaagtataa tccgatgccc agggcaagcg cggcggcgca
      361 gatgctgccc agttcccaca gcaggacggc ggcgatcttg cgcggaacca tgcccaacgc
      421 gcgtagcacg gcgaagctgg tcagccgacc ggaggcgttg agccaggcag aaaagagcgt
      481 cccagcgagc gccagcagca ggccgacggc aatgcccgcg cccagcaggc tcaccaggtc
      541 ggtatacagc gggtcgctct gatatttgtc gagcgtggcg cggaggtctt gcagatctgg
      601 ataggctttg cgcacactgg cgagcgaggc cgcgtcgccg cgcgtttgca gccaggcaaa
      661 attgggcgcg agtgttccgc cgccgctatc cttcatatag gcggtcgcgt agctctggta
      721 atcgaccagt aagccgtaat tcccggagtc gctgtacacg tcagggatgg cgtggacgat
      781 ggcagccaca atcatatgcg tattgaagtt gtcaaaagtg ggcaggatga acgatgagcc
      841 aaccgagagg ttcatgctct ttgccgtcgt gtcatcgacg atagcataaa ccacgtcatg
      901 cgcggcagca tcggcaccgt gcgccgccag cagcgacatc aggctcgata acgtttgatc
      961 cgagtagagt ggcgaccata acgccgtttg cgcgaaggtc tgtgtatcga ccgcgacgat
     1021 gtggttggtt acaaagtcgc cgttgttatt gaccgagatg gcctggtagc ccagcgttgc
     1081 agaggtcacg cccgccgttt gctggtagac agcctccgcc tgcgcgagac tctgcccctg
     1141 cggtggcggc gagatagagc cgctgaaatc cgcacccaca tggaagacgg ccgcgtcggc
     1201 gacgcgctgc tgctgggttg acacggaggt cagcatgaac agcccgaagg ccgttcccag
     1261 cgccagcagc aggatcatgc gcgcggcagg acgcggagcg cgttccatct gagcgaaggc
     1321 cagcgccgcg cttgctccac gcctcctcat ggtcagccgc gtccccaaac gcaagaaaag
     1381 tgggaagatg cgcaggaaga gcgtgatgcc ggcggctagc aggagtagcg gggcaatcag
     1441 cgccgccagt cccaggatga tcttcacatc aggctggtct gacaacgctg actgcgcaag
     1501 gtaggcatag gctgcatagc caacgcagac gagcagggag agcatgagat cgaggcgcag
     1561 gcgacgccag agcgatggtc gcatcgagcg cgcggattcg cggcggaagg ccagcatatc
     1621 catggccgat gcccgccgga tcgccacaat caccacgagt atcgcgatca acaccgcgcc
     1681 cagggcatac cagcccacac tcaacactgc ctgcaagggg ttgccggtca gaatgccgac
     1741 ggcggattgc tcgctggccg ggaaaagcca ttgtacgagc agcccgatca ggacgatggc
     1801 aagcaatggc ccggccagta gcgccgtcaa tcccaggccg atgccctgcg cagcgaacgc
     1861 gccgtagacg tggcgcagcg tcgcgccgcg actgcgcagc gtcgcgatgg tggctgcctg
     1921 ctgctcgatc aaggccgagg tcatcatgct gaccagaaag agtaccaggc cgagtatcag
     1981 gatcagcgag gacgagacaa cgatttcctc tacatagatg cgaatataat agtctggaag
     2041 aatatcgaac agagcgcccg aacccctggg aatgaagaat gtccctggta agttgctgag
     2101 actgttgtcg atgccggatt gtgccgtatc cgcgagcgac aacagcgttc cggcgttgct
     2161 ggccgtcacc tgcgaagaat tgaacgggta gctccaattc agttggaaga atggcacgtc
     2221 tcctccggct ttgaatgcgc gctttccacc agacacaagt tgtacctgca gcggcgctat
     2281 tgccggtaga atggccgcgt tggatgccag agccgggtag agattgccac tactgccgcc
     2341 ctgcgcctgg aaggtattgg aagcttccca gttcgctttc agcgcaaaga tgccgaccac
     2401 gcgcagcgtc cacgttcccc cgcttgctat gctctgcggc aggttcgcgc cgatgaccga
     2461 gccgacatgc acaccaagag cctgagcggt actctggatc aacgcaattt caatcacgtt
     2521 gttggctgcc tgttgtggca gtcgcccctg cacaatcgac aactcaccgg cgatctgatc
     2581 catcgcatag ctgtagatgc tgagcagtcg tttcgtgttc gacgcgccgc ttgtcgtccc
     2641 gccggaggtg atggactgga ccgtcagcgc gggcagttgc accgaaaagt gcgccgcgcc
     2701 gct
//
LOCUS       DASZAK010000673         4134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_159877, whole genome shotgun sequence.
ACCESSION   DASZAK010000673 DASZAK010000000
VERSION     DASZAK010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159877"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VGT44_11830"
     CDS             complement(<1..225)
                     /locus_tag="VGT44_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581531.1"
                     /translation="MIFIPRQKEKRRVSFGVMMTCACVVLVILLAACGTATGGGGTTP
                     GATANPQTPTVTPPNDLLTSGTLTVGSDTTY"
     gene            complement(460..912)
                     /locus_tag="VGT44_11835"
     CDS             complement(460..912)
                     /locus_tag="VGT44_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HEV2581532.1"
                     /translation="MTSSPVKTILDNRVLIAAFMAWAVAQISKTIYELIRFRKLRFNR
                     LVSAGGMPSSHSALVTGLATATGRLAGIDSIAFAIAFVLAGIVMYDAAGVRRAVSIQA
                     RILNQMIDEAYQGKPFAEKRLRELIGHTPIQVIVGGLLGVAVGLLLTA"
     gene            complement(928..1146)
                     /gene="xseB"
                     /locus_tag="VGT44_11840"
     CDS             complement(928..1146)
                     /gene="xseB"
                     /locus_tag="VGT44_11840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV2581533.1"
                     /translation="METISFEDAFAQLEAAVTALQDGRLPLERALNYYEQGMKLAKQC
                     QDLLQKAELRVQQLRLDASGEPATIPFE"
     gene            complement(1175..2410)
                     /gene="xseA"
                     /locus_tag="VGT44_11845"
     CDS             complement(1175..2410)
                     /gene="xseA"
                     /locus_tag="VGT44_11845"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV2581534.1"
                     /translation="MLFLDAPSYSVSQLSRYLKEALDSDPILQNVWVRGEVSGCKLHT
                     ATGHCYFTLKDIDAQLPCVFFKWARLRSGGPELRDGMAIAANGHISMYERDGKLQLYV
                     ERIEAVGDGALFLRFAQLKARLEAEGLFDEARKRPLPERPSVIGIVTSPQAAALQDML
                     RTLKTRYPLARVILSPAAVQGAEAPLSIAAALDLLNQHGEAEVIIVGRGGGSIEDLWA
                     FNEEVVARAIARSRIPVISGVGHETDITIADFVADYRASTPTAAAVAVVPNIEAWYEQ
                     IAVYQDDLGSLMEDYLIEKREAIEEASRDLARFSPQSQLESLSQRLDNAAALLQERMD
                     NLLSRKRDRLRSVQQLLNSLSPDRTVARGYAIVRRSADQRVVASAGQVQPGDALTIQV
                     TDGDIQTVVQSIHMQPDNA"
     gene            complement(2420..2956)
                     /locus_tag="VGT44_11850"
     CDS             complement(2420..2956)
                     /locus_tag="VGT44_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV2581535.1"
                     /translation="MDGNHFDYPLDMEVEETTASVISIEQLQRLVRLLDQSDVTELEL
                     ERAAEGVHLALRKFQAGESSQANMGQAAVSAVPAAAKGANADAAEAEKTTHNVVAPLV
                     GTYHVWAKPRGGALVAVGDKVKVGQLVGTIQSLNVLNEVETSVAGRVLELLVQDGQPV
                     EYGQVLMTIDSANGAKGA"
     gene            complement(2980..3537)
                     /gene="efp"
                     /locus_tag="VGT44_11855"
     CDS             complement(2980..3537)
                     /gene="efp"
                     /locus_tag="VGT44_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV2581536.1"
                     /translation="MISTGDLKKGVTFEFDGQPCALIEWQHVKIGRGGAIVRMKLRNL
                     RTGALFDRTCDAGDKFRRVDLERSTVTYQYQDGDQYHFMDTTTYDDIILTSEQLGSTI
                     NYLIDNLELDLILLDGAPISVEPPEKIVLRVEYTEPGFKGDTATGGSKPATTETGLIV
                     HVPLFISTGDMIRVNTTSGAYVERV"
     gene            complement(3551..>4134)
                     /locus_tag="VGT44_11860"
     CDS             complement(3551..>4134)
                     /locus_tag="VGT44_11860"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV2581537.1"
                     /translation="WEISRYMVELGADGISFDPIVASGPHGSMPHAEPGQRRIERGEL
                     ITIDMGARFQGYCADMTRTICLGEPKETRMTEVFDAVLNAMRVCEQHIRAGMTGIEAD
                     TLARDALNAAGLGEYYVHSTGHGVGLQVHEGPVLSQRAPKDKALPVGAVVTIEPGVYI
                     PGWSGARMEDCVLLTENGCEILTQSPTRLVIQE"
BASE COUNT          854 a         1243 c         1176 g          861 t
ORIGIN      
        1 atacgtggta tcacttccta cggtcagcgt acccgaagtc aacagatcgt tgggaggagt
       61 aacagtcggc gtctggggat tcgctgttgc accaggcgta gttccgccgc cgccggtagc
      121 ggttccacat gctgcgagca atatgactaa cacgacacag gcacaagtca tcatcacacc
      181 aaacgaaact cgtcgtttct ccttttgtcg gggaataaat atcataacgc tccctccttg
      241 ccgtaaacca ggatattctg aaaactacaa gttgtgtaca caatccgtta caccggtaga
      301 agcaatgctg ctcttccttg cctttacctg cttgtactac gttgctcaat agaaagtgta
      361 tcacaaaagc gaacaatcca tgtccacatt gcctcacatg tttgtgacat cgtaacaaag
      421 catgaccgta aaattgtcga gattatctac ctatcaccct tacgcggtca aaagcaggcc
      481 aaccgcgacg ccgagcaagc cgccgacgat gacctgaatg ggggtgtggc cgatcagttc
      541 gcgcaggcgt ttttcggcga agggtttgcc ctggtatgct tcatcgatca tctggttgag
      601 gatacgcgcc tggatgctga cggcgcggcg caccccggca gcgtcgtaca tgacaatccc
      661 ggccagcacg aaggcgatgg cgaatgcgat agaatcgatg ccggccagac ggcccgtggc
      721 ggttgccagc ccggtgacga gcgcggaatg cgaactcggc atcccgcccg cggagacaag
      781 gcgattgaaa cgcagcttgc gaaagcggat cagctcgtag atcgtcttcg agatctgcgc
      841 aaccgcccag gccatgaacg ccgcgatcag gacgcgatta tccaggatgg tttttaccgg
      901 gctgctggtc attgctcctc gcggtagtta ttcaaagggg atcgttgcag gttcgcccga
      961 tgcatcaagg cgcaactgct gcacgcgcag ttccgccttc tgcaacaagt cctggcattg
     1021 cttcgccagt ttcatcccct gctcataata attcagcgcg cgctcgaggg gcaggcgtcc
     1081 atcctgcaag gcagtaacgg ctgcctcaag ctgcgcaaag gcgtcctcaa acgatatggt
     1141 ttccataaat acctcctgag aatgaatcac atccctacgc attatccggt tgcatatgta
     1201 tcgactgcac gacggtttgt atatctccat ccgtcacctg gatggtaagg gcatcgccgg
     1261 gctgaacctg tccggcgctg gcgacgacgc gctgatcggc gctgcgccgc acgatggcgt
     1321 agccgcgcgc cactgttcgg tccgggctga gcgagttcag taattgttgc acgctacgca
     1381 gacggtcgcg cttccggctc agcagattgt ccatgcgttc ctgtaagagc gcggcggcgt
     1441 tatcgaggcg ctgggacagg ctttcaagct gtgattgcgg actgaaacgc gccaggtcgc
     1501 ggctggcctc ttcgatggct tcgcgcttct cgatgagata gtcttccatc aacgaaccca
     1561 ggtcatcctg gtagaccgct atttgctcat accatgcctc gatattgggt acgacggcga
     1621 cggcagcggc ggttggtgta gaggcgcggt agtcggccac gaaatcggcg atggtgatat
     1681 cggtttcatg cccgacgccg ctaatgaccg gaatacgcga gcgagcaatg gcgcgcgcta
     1741 cgacctcttc gttgaatgcc cacaaatctt cgatggaacc gccgccgcgc ccgacaataa
     1801 tcacctcggc ttcgccatgc tggttgagca ggtcgagcgc ggcggcgatg gagagcggag
     1861 cctccgctcc ctgcacggct gccggggaaa gtatgacgcg cgccagggga tagcgtgttt
     1921 tcaatgtgcg cagcatatcc tgcagggcgg cggcctgtgg cgaggtgacg atgccgatga
     1981 cggaaggacg ctctggcaaa gggcgtttgc gcgcttcatc gaatagtcct tcggcctcaa
     2041 ggcgcgcttt aagctgcgcg aaacgcagaa acagcgcgcc atcgccgaca gcctcgatac
     2101 gctcgacgta aagctgtagc ttgccgtcgc gttcatacat cgagatatgg ccatttgccg
     2161 caatcgccat gccatcgcgc agctccggcc cgccagaacg caagcgcgcc catttaaaga
     2221 aaacgcacgg caactgcgcg tcgatatcct tgagcgtaaa atagcagtga ccggtggccg
     2281 tgtgtagttt gcagccggag acctcgccgc gcacccagac attctgcaaa atagggtcgg
     2341 aatccagcgc ttccttcaaa taacgcgaga gctgcgaaac gctgtaggat ggagcatcga
     2401 gaaacaacat accgccccct tacgcgccct tcgccccgtt ggcgctatcg atggtcatca
     2461 acacctggcc atactccacc ggctggccgt cctggacaag caattcgagg acgcgcccgg
     2521 ctacgctagt ttcgacttca ttgaggacat tcaacgactg aatggtaccg accagctgac
     2581 ccactttgac cttatcccca acggccacca gcgcgccacc cctgggtttg gcccagacgt
     2641 ggtaggtgcc gacgagcggg gcgacgacgt tatgggttgt tttctccgcc tctgctgcat
     2701 cggcatttgc gccctttgct gcagcaggga cggcagagac ggcggcctgc cccatattcg
     2761 cctggctcga ctcgccagcc tggaacttcc gcaaggcgag atgcacgcct tccgcggcgc
     2821 gttccagttc gagttcggtg acatcgctct gatcgagtaa acgcaccagg cgctgcaact
     2881 gctcgatact gatgacgctg gcggtggtct cctcgacctc catatcgagc gggtaatcga
     2941 agtggtttcc gtccatacgc tccccctatc tcaacttact cagacacgct cgacgtacgc
     3001 gccgctcgtg gtattgacgc gaatcatatc gccggtcgag atgaacaacg gcacatgcac
     3061 gatcaggcca gtctcggttg tggcgggctt gctgccgccc gtcgcggtgt cacccttaaa
     3121 gcccggctcg gtatactcga cgcgcagtac gattttctcg ggcggctcaa ccgagatggg
     3181 cgcgccatcc agcaggatca ggtcgagttc caggttatcg atgagatagt tgatggtgct
     3241 gccaagctgc tcgctcgtca agatgatatc gtcgtaggtg gtcgtatcca taaagtgata
     3301 ctggtcgcca tcctggtact ggtaggtgac ggtgctgcgc tccaggtcga cgcgcctgaa
     3361 tttatcgccg gcatcgcagg tgcgatcaaa cagcgccccg gtgcgcaggt tgcgcagctt
     3421 catacgcaca atcgcgccgc cccgcccgat cttgacatgc tgccattcga tcagggcgca
     3481 gggctggccg tcaaattcaa acgtgactcc ttttttcaag tcaccggtag aaatcattca
     3541 cttgctcctt ttattcctgg atcaccagtc gcgttggtga ttgagtgagt atctcgcagc
     3601 cgttctcggt cagcaaaacg caatcttcca tacgcgctcc cgaccagccg gggatataga
     3661 cgcccggttc gatggtgacg acagcgccca ccggcaatgc tttatctttt ggcgcgcgct
     3721 gggaaagaac gggcccttcg tggacttgca gaccaacgcc atgaccggtg ctatgcacat
     3781 agtattcgcc gaggccggct gcgttgagag cgtcacgcgc cagcgtatcg gcctcgatgc
     3841 ccgtcatgcc cgcgcgaata tgctgctcgc agacccgcat ggcgttcaga accgcgtcga
     3901 acacctccgt catgcgtgtc tctttcggct cgcccaggca gatcgtgcgc gtcatatcgg
     3961 cgcaatagcc ctgaaagcgc gcgcccatgt caatcgtaat cagctcgccg cgttcgatgc
     4021 gccgctggcc tggttcagca tgaggcatgg aaccatgagg gccggaggcg acgatcgggt
     4081 cgaatgagat gccatcagcg ccaagctcaa ccatatagcg cgagatttcc cacg
//
LOCUS       DASZAK010000674         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_549241, whole genome shotgun sequence.
ACCESSION   DASZAK010000674 DASZAK010000000
VERSION     DASZAK010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549241"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1380
                     /locus_tag="VGT44_11865"
     CDS             187..1380
                     /locus_tag="VGT44_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2581538.1"
                     /translation="MSGTQTDHKVKMPSATDLYNIDHLLSEEERMVRDTVRKFVSERV
                     LPIIGEHFEEGTFPRELIPEIAGLGLLGMHLEGYGCAGLSAVCYGLACQELEAGDSGL
                     RSFVSVQGSLAMFPIYKYGSEEQKQRWLPPMARGEVIGCFGLTEPDSGSDPGSMRTNA
                     RRDGDTYILNGTKMWITNGGIADVAVVWAKTDDGIRGLLVERGTPGFTTSDVHHKLSL
                     RASITSELHFEECRVPAANMLPNVRGLRGPLSCLDEARYGIAWGATGAARACYEVALD
                     YARTRVQFGKPIGAFQLVQQKLAIIATELVKAQLLSLQLGRLKDEGLLHPVQISVAKR
                     NNVREALKTAREARSILGANGITLEYPISRHSNNLESVYTYEGTDDIHTLVIGQAITG
                     LNAFS"
     gene            1625..>2757
                     /locus_tag="VGT44_11870"
     CDS             1625..>2757
                     /locus_tag="VGT44_11870"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV2581539.1"
                     /translation="MISISKEVQRRFILGKQGLYPGRRWQGKDGVVQALRAGCVVQVD
                     PLNVVARSQDIVLYGRVTAYQPAHLDAALYTDRLLFDYGGAVMIHPMEELPYWRVVME
                     RKRHEPRWATFAREYGATIDEVLAAIRERGPLGTRAFTDETVLRKSSFRSGKVTSQAL
                     YYLWLAGELMTHSRQGFDRVYDIRDRVAPAAFNFAASPEEAENFFALKIFHGLGMATA
                     REFRGWFAGRIERSVDTAEAAAWLDAMQKAGQIVPGSLEGDAKATHFLLADDLRLLET
                     LRAGAIPDEWKSVDTTTSDDMILLAPLEIVNARGRGQLLFDFDYLWEVYKPAEKRRWG
                     YYTLPILYQDRLVARLDPRFERASGTLLILGFWLESDVTVDSR"
BASE COUNT          549 a          781 c          864 g          563 t
ORIGIN      
        1 gaggattcgc gccgataaat cggcggtgag cacggtaaac cggctcctac gggggggtgg
       61 ctctgtcgct gcctggttgt ttgcctaaaa gcgtttccta cgcccaaatg cataccgtga
      121 tttgacacgt acagcgcagt atgctgttct gctgcctgcc gcacagacga aagggtcgcc
      181 ttatttatgt caggaacgca aacagaccac aaagtgaaga tgccgtcggc caccgatttg
      241 tataatatcg accacctgct gagcgaggaa gagcgcatgg tgcgcgatac cgtgcgcaag
      301 tttgtcagcg agcgcgtcct gccgatcatc ggcgaacatt tcgaggaggg gacctttccg
      361 cgcgaactga tccccgaaat cgccgggctt ggcctgcttg gtatgcatct cgaaggctat
      421 ggctgcgcag ggctgagcgc ggtgtgctat gggctggcgt gtcaagagtt ggaggcgggg
      481 gatagcggcc tgcgatcgtt cgtctcggtg caggggtcgc tcgctatgtt ccccatctac
      541 aagtatggct ccgaggagca gaagcagcgc tggctgccgc cgatggcgcg cggcgaggtg
      601 atcggctgct tcggtctgac cgagccggat tccggcagcg accccggcag tatgcgcacc
      661 aacgcgcggc gcgacggcga cacatacatt ctgaacggca caaaaatgtg gattaccaac
      721 ggcggcattg ccgacgtggc ggttgtctgg gcgaaaacgg acgacggcat tcgcggcctg
      781 ctggtcgagc gcggtacgcc tggattcacc accagcgacg tgcaccacaa gctctccttg
      841 cgcgcctcca tcacctccga gctgcacttt gaggagtgcc gcgtgcctgc cgcaaatatg
      901 ttgcccaacg tgcgcgggct gcgcgggccg ctctcctgcc tggacgaggc gcgctatggc
      961 atcgcctggg gcgcgaccgg cgcggcgcgc gcctgctacg aggtcgcgct ggactacgcc
     1021 cgcacgcgcg tccagttcgg caagcccatc ggcgctttcc agctcgtgca gcaaaagcta
     1081 gccatcatcg cgactgaact ggtcaaggcg caactgctct ccttgcaact cggtcggctc
     1141 aaggacgagg ggctgctgca cccggtgcag atttccgtcg cgaaacgcaa caatgtgcgc
     1201 gaggcgctca agacggcgcg cgaagctcgc tccatcctcg gcgcgaacgg catcacgctg
     1261 gaatatccca tcagccgcca ctcgaacaac ctggagtcgg tctacaccta cgagggaacg
     1321 gacgatatcc atacgctggt gattgggcag gcgatcaccg gtttgaacgc cttctcctga
     1381 tgtgtcgcag ttgaacactc gtaggtgccg gtttaccgcg cacaccgccg atttatcggc
     1441 ctcgatgggc gtgaacgaca ggcactccgg gccgattatg gtttttaact tattaatgca
     1501 gcaatgttta cgctcaagat agccggatta ataagttaaa aaccataatc ggcggtgtgc
     1561 gcggtaaacc ggcacctacg agtctgtcaa ggttcactac gagcattttc gctagaggga
     1621 cacaatgatt agcatttcaa aagaggtaca gcgccgcttt atcctgggca agcagggtct
     1681 ctatcctgga aggcgttggc agggcaagga cggcgttgtg caggctcttc gcgcgggctg
     1741 cgttgtgcag gttgatccgc tgaatgtcgt ggctcgcagc caggacatcg tcctctatgg
     1801 acgagtcacg gcataccagc ctgcgcacct ggatgcggcg ctatatacag atcggctgct
     1861 gttcgattat ggcggcgcgg tcatgattca cccgatggaa gaacttcctt actggcgcgt
     1921 ggtgatggag cgcaagaggc atgaaccgcg ctgggcaacc tttgcccgcg aatatggcgc
     1981 caccatcgat gaagtgcttg cagccatccg ggaacgcggc ccgctcggta cccgcgcgtt
     2041 tacggacgaa actgttttgc gtaagagcag cttcagatcc gggaaggtga cgagccaggc
     2101 gttgtattat ctctggttag cgggcgagct gatgacgcac agccggcagg gatttgaccg
     2161 cgtctacgat attcgcgacc gcgtggctcc cgccgccttt aatttcgctg catctccaga
     2221 agaagctgaa aactttttcg cgctcaaaat atttcacgga ctcggcatgg ccacggcgcg
     2281 cgagtttcgc ggctggtttg caggcaggat tgagcgaagt gttgatacag cagaggcggc
     2341 ggcatggctg gatgcgatgc agaaagccgg tcagattgtc ccgggcagcc tggaaggtga
     2401 tgcgaaagcc actcactttc tactggccga tgatctgcgg ctgttggaaa cgctgcgggc
     2461 gggcgctatt cctgacgaat ggaagagcgt tgatacaacc acctcggacg atatgatctt
     2521 gcttgcgccg cttgaaatag tcaacgcgcg cggacgtgga cagcttctct tcgactttga
     2581 ctatttgtgg gaagtgtaca aaccggcgga aaagcggcgc tggggctact ataccctgcc
     2641 gatcttgtac caggacaggc tggtggcgcg gctcgatccc cggttcgagc gcgcttcagg
     2701 gacgctgctc atcctggggt tctggctgga aagcgatgtc accgttgaca gccgctt
//
LOCUS       DASZAK010000675         8167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_51921, whole genome shotgun sequence.
ACCESSION   DASZAK010000675 DASZAK010000000
VERSION     DASZAK010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51921"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            493..1239
                     /locus_tag="VGT44_11875"
     CDS             493..1239
                     /locus_tag="VGT44_11875"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2581540.1"
                     /translation="MQDEKDKRTDAIVDEDERVTDTIASQPVCTVEEPVQRVEAVDVA
                     DFETIFLRFQGPISNYIYHLIGNREQAYDLAQDVFVKAYKALLNGTVVQPGALSAWLY
                     RIAANTTTDSLRRRRLIAWLPLSLFNEDRGIGAGVLSETSGSSSENFSANGDDGEGRG
                     SSVSSMYERNGYDGGLFENRVADREIVERIFQQLPPKYASCLWLYEHDGHSCAEIADV
                     LNISASAVKMRLMRARERFITLYRQEVGDK"
     gene            1236..3047
                     /locus_tag="VGT44_11880"
     CDS             1236..3047
                     /locus_tag="VGT44_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581541.1"
                     /translation="MNCEQARSLLAAHRELKNGIVDTTELDVHLERCPSCRQVLARST
                     FIGKSLRALPEIETPPEMYTQLMHKLANEHLQFMQRATPGSITTPEFLKPYLQEHAQS
                     RLASHPMSALSTAATGPLPIIHAKRRGRPLHTHRAPMSQFAILGLAAAFLVLLMMGGV
                     TSLVFLAQHNAQQLARVTARNVDVIQNVDIHEANYATATLYPHIASAVANSDAIYYTA
                     YTDSASPQWMLLQMDRNKQQTSSPLLATLSSEPMVVLGSSSDWLVWLQYGTPKAKIYR
                     NVSNGYSHAIVTPWNLYALSLTQLRNSQQANGNFPSPQLLLKGIYDPTTAPSWVHTPV
                     QGIWFMQDTLLIAALDASGTSHLLSSQLNLAGKSTLTTLATAASGHIYTSPTADSSAT
                     EIFWADEWISNAGALSSNILQLLQVNAPGSVHPTHGHWNKTEPQVLQQALFRGDSVSF
                     HPQITDDMLFWINSPQPPSSDIATSTPTQTSASIVPRLDAAYYAPPLDATVRGQVMMQ
                     PIAGDILSPPTTLASTGQAYSLQSGTDFILWQTDKGYVMYDVPSQSNVTVGTVLNEAG
                     FLAVNNNTAVWIKYASSTTTPASVLPAVHIYAFNWPK"
     gene            3156..4016
                     /locus_tag="VGT44_11885"
     CDS             3156..4016
                     /locus_tag="VGT44_11885"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2581542.1"
                     /translation="MTRDENSENTYIIDSESGAEMARLIDQDHSMNEAMGSWFPEPID
                     LTEIHRVLDVACGPGGWAQEVAWRYPHLQVVGIDISQAMIAYAGQRALVQRLDNASFQ
                     VMDARAPLSFPDGAFDLVNARFISAFLPRGEWPGVIRDFARLTRPGGSIVLTEVDIFS
                     PDNTNSPALEEWNALCREAVYRAGLYTSTGIRVTSILDQLLQEAGCQDIRQQWHTLDF
                     SAGTPAHATVCLNLEFALKLVQPFLLKMVQTTQARLNELYTQGLHEMNRDDFHGLTTF
                     LTAWGTRSAI"
     gene            4074..5249
                     /locus_tag="VGT44_11890"
     CDS             4074..5249
                     /locus_tag="VGT44_11890"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEV2581543.1"
                     /translation="MMGSNAGMPALSWVDITARRMARHGLSAPFKDARPADIVRAMCG
                     AHAQVVSAAFWSIGLRSASLTEPEVRDALWTERSLVKTFGPRGTVHLLTAHDLPMWAG
                     ALSAIPPSHNGHSKEVRLTREQTDEVVNAIAVALFDAELTIDELSEAVIAAAGQWAGE
                     RVMPAFNGMWPRWRMALALAGFRGALCFGPNRGPKVTYTNPQRFLPGFAPVEGRRALA
                     WLLKSYLYAYGPATPQHFAQWLNAPRSWAKKLFDSLSGELEPVEVNGSLAWVVAGDTA
                     FPSSRLQSVRLLPYFDAYTVGSQPRELLFPGRAAQRALTPSGQAGNYPVLLIDGTVAG
                     VWHQRRSGRCLDITVEPFEQLTAKERRDLDGQVELMGTFLKAQPRLTIGTVSAGAHA"
     gene            complement(5382..6029)
                     /locus_tag="VGT44_11895"
     CDS             complement(5382..6029)
                     /locus_tag="VGT44_11895"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEV2581544.1"
                     /translation="MDITPASQVPSGSKAQAHHEYKNLLEGIHFKFPTFKHEHPPVIN
                     VNEVADEQLTVGQKVADTVAGTVGSWRFIIIQSIVLAIWLALNITGIVFRWDPYPFIL
                     LNLALSFQAAYAAPFVMMSQNRQSEKDRLMAESDYHCNTKGEEEVRNIMEHLDHQDVV
                     IMQIIKRMEAQHEEVMQHLARLDPEMARRLGMDIQEVSKELVEEATEGGTGGTHK"
     gene            complement(6064..7212)
                     /locus_tag="VGT44_11900"
     CDS             complement(6064..7212)
                     /locus_tag="VGT44_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2581545.1"
                     /translation="MDFTLNDEQQAIRDTCRDFAEQEIKPLAEEMDRTGEFPYELVRK
                     MGELGLLGLPFPEEYGGAGADFLSYCLAMEEISRGDVSVGITMEAHTSLGASPFYLFG
                     SEAQKQQHLVPLASGKQLWAFGLTEPGAGSDSGGTQTRAVLRKGSWHINGSKSFITNA
                     GTEMTGGVTITAVTDDRPGGRNEITNIIVPKGTPGYIIGNPYKKMGWKASDTRPLTFE
                     DCIVPEESILGRRGDGFKQFMQILDAGRVAIAALSVGLAQACLDAALAYAKERKQFGK
                     SISKFQAIQFKLADMDTEIELARLMYYKAAWLHMEGRPYSREASMAKLFASETAKRAA
                     DQAVQIHGGYGFMDEYPVSRYWRSVKINEIGEGTSEVQRMVIARLLGC"
     gene            7318..>8167
                     /locus_tag="VGT44_11905"
     CDS             7318..>8167
                     /locus_tag="VGT44_11905"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2581546.1"
                     /translation="MSLTVEILTEAELDAANEVIKAAYNNPHGRAETLRRYLALQPDG
                     TCVVKYDGQVVGFGGAIHYGAFAYIGMMSVHPAMQRRGIGQVIMDYLLDWLDRRACPT
                     ILLDATPAGRPLYERCGFIEKDTTVVLRYTQVEMPAQTASEQVYKANASDLPELIAFD
                     APAFGAERRALLAAYYADDPRRVLIARDANGRLNGYLIAQQSVIGPWVARGAADAERL
                     LLRALALPFDDHPRVFVSQHNTAALQLLARHGFTQQRTLSHMRRGEHIQRSRHTMIYG
                     QTSLGFG"
BASE COUNT         1782 a         2480 c         2186 g         1719 t
ORIGIN      
        1 tggtcgccct ggttaccctc cacctccttc gcaggccgct tcgtccatgg taggggcgac
       61 cctcgcgctc gccctggaaa atggtctcgc gctcgccctg gtaccctttg ctctagcccc
      121 tgcttcgtcc agaatgctat agttatattg acaggcattt gcgtgaattg acacacgcta
      181 tgcaacaagg ggcacgggac atccgtatct tataggggga gattgtggtc ggcaggcaac
      241 gcgaatagca ggccgacccg gctcggcgca tcataggaaa acaggcttat ttgtctactg
      301 attcacagta aatgctgacg aaatatggta tgctaatcac gtctcaaaca atattatgta
      361 cgaacttgaa ctgctttgca gcatatgata aggcttatgt aaccaatgga tacctcaatc
      421 cgtttctaca attcagagag agggttgctc tcaacagtgg agtcagagac ccgaagttat
      481 gcgtccagct ccttgcagga cgaaaaagac aagcgaaccg acgccattgt ggatgaggat
      541 gagcgtgtca ccgacacgat tgcctcacag cccgtttgca cagtcgaaga gcctgttcag
      601 cgggttgaag cggtagacgt agcggacttt gaaacgatct ttcttcgctt tcaaggaccg
      661 atatccaact acatctacca cctgatcggc aatcgcgagc aggcttacga ccttgcgcag
      721 gatgttttcg tcaaggcgta taaggcgctc ttgaacggga cggtcgtcca accgggcgcg
      781 ttatccgcct ggctctaccg catcgcagcg aacacaacga cggattccct gcgaaggcga
      841 cggttgatcg cctggctgcc tctttcgttg ttcaacgagg atcgcggcat cggcgccggt
      901 gtcctctccg aaacgagcgg ctcttcctcc gagaatttta gcgcgaatgg tgacgatggc
      961 gaagggcgcg gcagctccgt gagcagcatg tatgagcgca acggttacga tggcgggcta
     1021 tttgagaatc gcgtggccga ccgcgaaatt gtagaacgca tcttccaaca gttgccgccc
     1081 aaatatgcct cctgcttatg gctctacgag catgatggtc attcctgcgc agaaatcgcc
     1141 gacgtcctga atatcagcgc ttcagcagta aaaatgcgcc tgatgcgcgc gcgcgaacgg
     1201 ttcattacct tataccggca agaagtgggt gacaagtgaa ttgcgagcaa gcgcggtcat
     1261 tactggccgc acatcgagag ttgaaaaacg gcatagtcga tacgactgaa ctggacgttc
     1321 acctggaacg ctgcccatcc tgccggcagg tcctggcgcg ttcgaccttt attggcaaaa
     1381 gcctgcgcgc tttgccagag atcgagacgc ctcccgagat gtacacgcaa ctcatgcaca
     1441 aactcgccaa tgaacatctg caatttatgc aacgggcaac ccccggcagc atcacaactc
     1501 ccgaattttt gaaaccttat cttcaagaac acgctcaatc caggctcgca tcccatccga
     1561 tgagcgctct ctcaaccgct gcaaccgggc cattaccgat cattcatgcc aaacgcaggg
     1621 gacgccccct tcatacccat cgtgcgccga tgagccagtt cgccattctg ggactggccg
     1681 ccgcattcct ggtactcttg atgatgggcg gcgtcacgtc gcttgtcttc ctggcccagc
     1741 ataacgcgca gcaactcgcc cgcgtcaccg cccgcaatgt tgatgttatt cagaatgtcg
     1801 atatccacga ggcgaactac gccaccgcga ccctctatcc ccatatcgcc agcgccgtcg
     1861 ctaatagcga tgccatctac tacaccgcgt acactgatag cgcttcccca caatggatgc
     1921 tgcttcagat ggatcgcaat aagcagcaga ccagctctcc tctgctcgca actctcagca
     1981 gtgagccgat ggtagtgctt ggcagctcgt ctgactggct ggtctggtta caatacggca
     2041 cacccaaggc gaaaatctat cgtaatgtct cgaatggata tagccacgct atcgtgacgc
     2101 cctggaacct gtacgcgctt tctctcacgc aactccgcaa tagccagcag gccaatggaa
     2161 actttcccag ccctcaactg ttgttgaaag gcatctatga cccgacaacc gcgccatcct
     2221 gggtccatac gccggttcag ggtatctggt tcatgcaaga cacgctgtta attgccgcac
     2281 tcgatgcgag tggcacttca catctgctct cgtctcaatt gaatctggcc ggcaaatcca
     2341 ccctgaccac gctggcaaca gcagcatccg ggcatatcta cacctcgcct acagctgata
     2401 gcagcgctac cgaaatattc tgggccgacg agtggatctc caacgccggt gccctgagta
     2461 gcaatatctt gcaactgctc caggtcaacg cgcctggctc tgtgcatcct actcatggac
     2521 actggaacaa gactgagccg caggtcctac agcaggcgct tttccgcggc gatagtgtat
     2581 ccttccaccc ccagattacc gatgatatgt tgttctggat caacagccca caacccccct
     2641 cgtctgacat agcaacctcg acgccaacgc aaacgagcgc ctctattgtt ccgcgcctcg
     2701 acgccgctta ctacgccccg ccgctcgatg caacagtgcg cggccaggtc atgatgcagc
     2761 ccatcgcggg cgacatcctt tcaccgccca ctaccctggc ctcgaccggc caggcgtact
     2821 cgctccagag cggcacagac ttcattctct ggcaaaccga caaaggctat gtgatgtacg
     2881 atgtgccgtc gcaaagcaat gtgacggtcg gcaccgttct gaacgaagct ggtttcctgg
     2941 ccgtcaataa caacacagcg gtctggatca agtatgcgtc gagtacaacg acccccgcca
     3001 gtgttctccc cgccgtacat atctacgcct tcaactggcc aaagtaagca tctcaccaat
     3061 agccgaagag catattgctg aataacgcga gccatgttat catgccagaa gaggcggaac
     3121 acgcaaaacc agcagagaac agcaggtgat gaacgatgac cagggatgaa aacagcgaga
     3181 atacctacat catcgactcc gaaagtgggg ccgaaatggc gcggttgatc gatcaggatc
     3241 atagcatgaa cgaggctatg ggaagctggt tccctgagcc tatcgatctt accgagatac
     3301 atcgtgtgct ggatgtcgcg tgtgggccgg gaggatgggc gcaagaagta gcctggcggt
     3361 accctcacct tcaggtggtc ggcatcgata tcagccaggc catgatcgcc tatgccggcc
     3421 agcgcgctct ggtccaacgg ctggacaacg ccagttttca ggtgatggat gcgcgcgcgc
     3481 cactcagctt ccctgacggt gctttcgacc tcgtgaatgc tcgcttcatc tcagcatttc
     3541 ttcctcgtgg tgaatggcct ggcgtcatcc gcgacttcgc gcgactcact agaccaggcg
     3601 gtagcattgt cctcaccgaa gttgatatat tcagcccgga caataccaat agtcccgccc
     3661 tggaagaatg gaacgcgctg tgccgcgagg cggtctaccg cgctggcctc tataccagca
     3721 cgggaatacg ggtgacatcc atcctcgacc agttgttgca ggaggcgggg tgccaggata
     3781 tccgccagca gtggcacacg ctggatttct cggcgggaac accggcgcat gcaaccgtct
     3841 gtctcaatct cgaattcgcc ctgaagctcg tccaaccttt cctgctcaaa atggtgcaga
     3901 ccacgcaagc acgcctcaac gaactgtata cacagggtct gcatgagatg aatcgtgacg
     3961 acttccacgg cctgacgact tttttgacgg catggggaac ccgaagcgca atataagtca
     4021 tacggagaga tgaagcgtcg agccgaagca gcacagagca ggggtggcac actatgatgg
     4081 gatcaaacgc tgggatgccg gccctgtcat gggtcgatat taccgcccgc cggatggctc
     4141 gccatggcct cagcgctccc ttcaaggacg cccggccggc ggatatcgtg agagccatgt
     4201 gcggcgcgca cgcccaggtg gtgtccgccg ccttctggtc catcgggctg cgaagtgcct
     4261 ccctcaccga gccagaggtc agagatgctt tgtggaccga acgcagtctc gtcaaaacgt
     4321 tcgggccgcg tggcacggtt caccttctca ccgcgcacga tctgcccatg tgggctggcg
     4381 ccctttcggc gatcccgccc tcccacaacg gtcattcgaa agaggtacgg ttgactcgtg
     4441 agcaaaccga tgaagtcgtc aacgccatcg ccgtcgcact tttcgatgct gagttgacca
     4501 tcgacgaact gagtgaagcg gtgattgccg ccgccggaca atgggccggc gagcgggtca
     4561 tgcctgcctt caacggcatg tggccccgct ggcgcatggc tctggctctg gctggctttc
     4621 gcggtgcgct gtgcttcggc cccaatcgag gccccaaagt cacctatacc aacccgcaga
     4681 ggtttctgcc tggatttgct cccgttgaag gacgaagagc cctcgcctgg ctcctcaaga
     4741 gctatctgta tgcctacggg ccagccacgc ctcagcactt cgcgcagtgg ctcaatgcac
     4801 ctcggagttg ggcgaagaaa ctgttcgact ctctctctgg tgaacttgag ccagtggagg
     4861 tcaacggcag cctggcatgg gtggtggctg gagatacggc tttcccgtcc agtaggcttc
     4921 aaagcgtccg gctgcttccc tacttcgacg cctacaccgt cggcagccag ccgcgtgaac
     4981 tgctctttcc cggacgagcc gcacaacgcg cgctgacccc gagtgggcag gcaggaaact
     5041 acccggtgct actcatcgat ggaaccgtcg ccggggtctg gcatcagcgg cgctctggcc
     5101 gctgtcttga tatcacagtg gagccgtttg agcaactcac tgccaaagag cgccgcgacc
     5161 tcgatgggca ggtggagctt atgggaacct tcctcaaggc tcaaccacga ttgacgatcg
     5221 gtacggtcag tgcaggcgca catgcatgag cttgcagaca tcccattgcc aggtgaacct
     5281 tccgcagctc gtatcgataa ctggctgcac gcgagcggac taggtttgaa gagaaaacgt
     5341 ctctcataga aaatgctaac agtaattgtt cataataggg cttatttatg agttccgcca
     5401 gtaccgccct ctgtcgcctc ctcgacaagc tccttgctaa cctcctggat gtccattccc
     5461 aagcgacgag ccatttctgg atcgagccgg gcgagatgct gcatgacttc ctcgtgctgg
     5521 gcctccatgc gtttgataat ctgcatgatg accacgtcct ggtggtcaag gtgttccatg
     5581 atgttgcgga cttcttcttc gcctttggtg ttgcagtggt aatcgctttc ggccatcaac
     5641 cggtcctttt cggattggcg attctgggac atcatcacaa acggcgcggc gtaggccgct
     5701 tgaaaactca acgctaaatt gagcagtatg aacgggtaag ggtcccagcg aaaaacgatg
     5761 ccagtaatgt tcaaagccaa ccagatggcg agaactatgc tctggatgat gatgaaccgc
     5821 caacttccta cagtccccgc caccgtatct gccaccttct gccccaccgt caactgttca
     5881 tccgcgacct cgttgacatt aataacagga ggatgctcat gcttaaacgt aggaaacttg
     5941 aagtgaattc cctctaaaag attcttatac tcatggtggg cctgcgcttt actgcccgaa
     6001 ggaacttgag aagcgggtgt aatgtccata agattactcc ttaatagtag ccaacaatag
     6061 tagtcaacat cccaacagcc gggcgattac catgcgctgc acctcgctgg tgccttcgcc
     6121 gatctcgttg atcttgacgc tgcgccagta gcgcgagacc ggatactcgt ccatgaagcc
     6181 ataaccgccg tggatctgca cggcctggtc ggcggcgcgt ttggcggtct ccgaggcgaa
     6241 cagcttggcc atcgatgcct cgcggctata gggtcggccc tccatgtgca gccaggcggc
     6301 cttatagtac attaaacgcg ccagctcgat ttcagtatcc atatcggcca gcttgaactg
     6361 gatcgcttga aacttgctga tcgacttgcc gaactgtttg cgttccttcg cgtaggcaag
     6421 cgccgcatcc aggcaagcct gcgccaggcc gaccgagagc gcggcaatag cgacacgccc
     6481 ggcatccaga atctgcataa actgcttgaa accatcgccg cgccgtccca ggatactctc
     6541 ctccggcacg atacaatcct cgaacgtcag cgggcgtgta tcggaggctt tccagcccat
     6601 tttcttgtaa ggattgccga tgatgtagcc cggcgtcccc ttcggcacga tgatgttggt
     6661 gatctcgttg cgcccgccag gtcgatcgtc ggtcacagcc gtaatcgtca ccccacccgt
     6721 catctccgtc ccggcattgg taatgaagct cttggagccg ttgatatgcc aggagccttt
     6781 gcgcagcacg gcgcgcgtct gcgtcccacc agaatcggaa ccggcgccag gttcggtcag
     6841 gccgaacgcc cagagttgct tgccgctcgc aagcgggaca aggtgctgtt gtttctgcgc
     6901 ctcgctgcca aagaggtaaa acgggctggc gcccaacgaa gtatgcgcct ccatcgtaat
     6961 ccccaccgaa acgtcgccgc gcgagatctc ttccatcgcg agacaatagg agaggaagtc
     7021 cgcgcccgcg ccgccatact cctccgggaa gggcaacccc aataaaccta gctcgcccat
     7081 ttttcgcacc agctcgtagg gaaactcgcc cgtgcgatcc atctcctcgg ccaggggttt
     7141 tatttcttgt tcggcaaagt cacgacacgt atcacgaatc gcctgttgct cgtcattgag
     7201 cgtaaagtcc atagtatctt cgtcactttc tctggctggt aatctaccct caaagaccat
     7261 tttcgtctca ttattatacg agatggagac aagttattct caaagaggtt atcattgatg
     7321 agtttgaccg tagagatact aaccgaagca gagcttgatg cagcaaacga ggtgatcaaa
     7381 gcggcctaca acaacccgca tggcagggca gaaacgctgc gccgttacct ggcgctacaa
     7441 ccggacggca catgcgtcgt caagtatgac ggccaggtcg tcggctttgg cggagcgatc
     7501 cactacggcg cattcgccta tatcggcatg atgtcggtcc atccagccat gcagcggcgc
     7561 ggcatcggac aggtcatcat ggactacctg ctcgattggc tcgacaggcg cgcgtgtccc
     7621 accatcttgc tcgatgccac acccgccggg aggccgctgt acgaacgctg cggctttatc
     7681 gagaaagata ccaccgttgt cctgcgctac acgcaggttg agatgccggc gcaaaccgcg
     7741 tccgaacagg tttataaagc gaatgcaagc gacctgcccg aactgatcgc gttcgacgcc
     7801 cccgctttcg gggcagaacg cagagcgttg ctggccgcgt attatgccga tgatccccgg
     7861 cgtgtcctga tcgcgcgcga tgccaacgga cgattgaacg gctacttgat tgcccagcaa
     7921 agcgtcatcg gcccctgggt tgcgcgcggg gcggcagacg cggaacggct gctgctgcgc
     7981 gcgcttgccc taccgttcga cgaccatccc agggtgtttg tctcgcagca caacaccgcc
     8041 gctttacaac tcctggcgcg ccacggcttt acgcagcaac gcaccctgag ccacatgcgc
     8101 cggggagagc atattcaacg cagccgccac acgatgatct acgggcaaac cagcctgggc
     8161 ttcggct
//
LOCUS       DASZAK010000676         4518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_230051, whole genome shotgun sequence.
ACCESSION   DASZAK010000676 DASZAK010000000
VERSION     DASZAK010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4518
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230051"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VGT44_11910"
     CDS             complement(<1..465)
                     /locus_tag="VGT44_11910"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HEV2581547.1"
                     /translation="MMDELKPARDAERSPRALDRETGASIAKADAPIGVFDSGAGGLT
                     ILAALRQELPRERYVYVADTAHCPYGTRTEAEITALSIEISRFLIEQGAKLIVVACNT
                     ASQAALAALRATFPFISFVGVVPAVKPAVSKTRNGRIAIAATNQAARSIYLSQ"
     gene            complement(480..2147)
                     /locus_tag="VGT44_11915"
     CDS             complement(480..2147)
                     /locus_tag="VGT44_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV2581548.1"
                     /translation="MEPHTSSGAQQWRETTARQAKQRAPERQSAFMTSSNIEIPDLTT
                     EEDLRDFDARAQLGYPGEFPFTRGVQAAMYRSKLWTMRQYAGFATAEESNKRYHYLLA
                     QGTTGLSVAFDLPTQMGYDADHVLSQGEVGKVGVSISSLEDMETLLGGLPLERISTSM
                     TINATASILLALYIAVAKKQGADVRKLSGTVQNDVLKEYIARGTYIYPPQYSMRIVTD
                     IFSYCTENLPRWNTISISGYHIREAGSTAVQEVAFTLSNAIAYVQAALDAGLNVDAFA
                     GQISFFFNSHNNLLEEVAKFRASRRMWARIMRERFHAQDARSMMLRFHTQTAGSTLTA
                     QQPDNNIVRTAYQALAAVLGGTQSLHTNSKDEALSLPSEESVRVALRTQQILAYESGV
                     ADVVDPLAGSYYIEYLTDAIERQAWDYIERIDALGGSVHAIERGFMQAEIEQAAYDYQ
                     RALEEQKAIVVGVNRFTVDEGERPALMRVDPEVGRRQAAKLAALRERRDSARVQQALR
                     DLEAGAEGSENLMPLIIAAVEAYATLGEISDTMRRVFGEQREFRSME"
     gene            complement(2871..3212)
                     /locus_tag="VGT44_11920"
     CDS             complement(2871..3212)
                     /locus_tag="VGT44_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581549.1"
                     /translation="MELQVRQVPDMRERANRISSFLTVQLIALIGWIIVQSLLFVSPV
                     SNSLPFIVKMIIETVWWIVTLVIMFYLFRRIYDRFVKAVLDLEDANRRLRKATNDLLT
                     EMANPSEQEQQ"
     gene            3924..4454
                     /locus_tag="VGT44_11925"
     CDS             3924..4454
                     /locus_tag="VGT44_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581550.1"
                     /translation="MKKQNAFPVSRPVSILLLAALAVLIIACSSAAKQGTSSSSNSSS
                     SQTYTSIPIVSTPEPEQPALPGAVSVYVTLQEFTITSSVRTFHAGIHYYFIVTNRGHA
                     VHEFMMMRDTANGTPISFDEDSSTMFIHIEQVFPGTTLHMNFTFTPSLIGKSEIACLM
                     RGHYAAGMHLPIVVAV"
BASE COUNT          967 a         1314 c         1165 g         1072 t
ORIGIN      
        1 ctggctcagg tagatcgacc tggcggcctg gttcgtggcg gcaatcgcga tgcgcccatt
       61 cctggttttg ctcacggcag gcttgacggc aggcacgacc ccgacaaacg agatgaaggg
      121 aaacgtggcg cgcagcgcgg ccagcgccgc ctgcgaagca gtattgcagg ccaccacaat
      181 cagcttcgcg ccctgctcga ttaaaaaccg gctgatctct atcgacaacg cagtgatttc
      241 tgcttctgtg cgggtgccat acgggcagtg cgctgtatct gccacataga cgtaacgctc
      301 gcgcggcagt tcctgtcgta atgcagccag gatcgtcaat ccaccggcgc ccgaatcaaa
      361 taccccgatg ggagcatccg cctttgcgat tgatgcccct gtttcccggt caagcgcgcg
      421 cgggctgcgt tccgcgtcgc gcgcgggctt taactcatcc atcatagaaa cgttatactc
      481 tattccatgc tacgaaattc tcgctgttcc ccaaagaccc gccgcatcgt atcgctgatc
      541 tctcccagcg tcgcgtaggc ttcgaccgcc gcgataatca gcggcatcag gttttccgag
      601 ccctccgcgc ctgcttccag gtcgcgcagc gcctgctgga cacgcgcgct atcgcgccgc
      661 tcgcgcagcg ctgccaattt tgccgcctgt cgcctgccaa cctccggatc gacacgcatg
      721 agcgcaggtc gctctccctc gtccacggtg aagcggttga ccccgaccac gatggccttt
      781 tgttcttcca acgcgcgctg gtaatcgtag gccgcctgct cgatctcggc ctgcatgaag
      841 ccgcgctcaa tagcatgaac gctgccaccc agcgcatcga tgcgttcgat gtaatcccat
      901 gcctgccgtt cgatggcatc ggtcagatac tcaatataat acgaaccggc cagcggatca
      961 accacatcag cgactccgct ctcgtaggcc agaatttgct gtgtgcgcag cgccacccgc
     1021 acggattctt cgctgggcag cgataacgcc tcatccttcg agttggtgtg cagcgattgt
     1081 gtgccgccca gcacggctgc cagcgcctgg taggctgtgc gcacaatatt attgtcgggt
     1141 tgttgcgccg tcagcgtaga ccctgccgtc tgcgtgtgaa agcgcagcat catcgagcgc
     1201 gcgtcctggg cgtggaagcg ctcgcgcatg atgcgcgccc acatgcgccg cgacgcgcgg
     1261 aacttggcca cctcctccaa caggttatta tgactgttga agaaaaagga gatctgcccg
     1321 gcgaaggcat cgacgttcag gcccgcgtca agcgccgcct gcacataggc aatcgcgttg
     1381 gaaagtgtaa aggccacttc ctgcacagcc gtcgagccgg cctcgcggat atgatagccg
     1441 ctaatgctga tagtgttcca gcgcggcagg ttctccgtgc agtagctgaa aatatccgta
     1501 acgatgcgca tggaatactg tggcgggtag atatatgtgc cgcgcgcgat atactccttc
     1561 agcacatcgt tttgcaccgt tcctgaaagc ttgcgaacat ccgcgccctg cttcttcgcc
     1621 accgcgatat ataacgccag cagaatactc gccgtcgcat tgatcgtcat cgaagtgcta
     1681 atccgctcga gcggcagccc gcccagcagt gtctccatgt cttccaggct ggagatagag
     1741 acgcccactt tccctacctc gccctgcgaa agaacatgat ccgcgtcgta tcccatctgc
     1801 gtcggaaggt cgaacgcgac cgaaaggccg gttgtcccct gcgccagcag gtaatgatag
     1861 cgcttattcg actcctccgc cgtcgcgaac cctgcatact ggcgcatcgt ccacagcttc
     1921 gaacgataca tcgcagcctg gacgccgcgc gtaaacggga actcgccagg atagccgagt
     1981 tgggcgcggg catcaaagtc ccgcaggtct tcttccgtcg taagatcggg tatctcgata
     2041 ttggaactgg tcataaacgc cgattgtcgc tccggcgcgc gctgcttcgc ctgccgcgcc
     2101 gtcgtctcgc gccactgctg cgcgccactg ctggtatgcg gctccatgcg tcacctccct
     2161 gttatgcccc tgtattatag cattttcagg ggagggtttg ttgcgcattc gttgaacggc
     2221 tctctgagcg actcggcgat ttttggtgta tgccattttg tctactgatg tctaccgttt
     2281 tcgctggttt tctctcggct ttgctgtcaa ctttgctgta cgatttttct tcgtgatcgg
     2341 cttactggct agctttatcg ttaaatggct agtcatgtgt ggagaaagtg aggcgatgcg
     2401 atgacaggac agaagaacgg gatgatgtca cattccttat tgttgactat tccagaggcg
     2461 gtggagactg aaccaaagac gagggcgagt aagcgaaata taatgctccc tgatgaggtc
     2521 ttatcgatgc tagccaaaca tcgggaaagg caagggcaag agctaaagct gctacaaagt
     2581 ggtttgagcg agggctggtt ttctgtaatc aatctggcgg ttttctcgtt ccctggtacg
     2641 tgaatacttt gttcgacaag ttgttggctc aagcgggctt gccaaagatg cgctttcatg
     2701 atcttcgtca tagtaaggcg acgatcttgt tagcggcggg agtgcatcca aatgaattat
     2761 ttgcgccagt tatacaacga gtcggaaacg atcaagacta tgggcgaaaa tcggctttgt
     2821 agttataaat tacttgcacc cctcgcagct ccgcctgtcc tgtcatacca ttattgttgt
     2881 tcctgctcgc tcggattcgc catttctgtc aggagatcgt tcgtcgcctt cctcagcctc
     2941 ctgttggcgt cctccagatc gagtaccgcc ttcacaaagc ggtcatatat ccgcctgaac
     3001 aggtaaaaca taataaccag ggtaacaatc caccagacgg tctcaatgat catcttaacg
     3061 ataaaaggca ggctgttgct cacgggggaa acgaacagga gtgattgcac gataatccaa
     3121 ccaatcagtg caatgagttg gaccgtgagg aatgatgaga tgcgatttgc gcgctcgcgc
     3181 atgtctggca cttgccgcac ttgcagttcc atcgaagaat accctcctct caaacgaaga
     3241 acacacttta cacattttgc cagggtactg tagacctctt gcataaggtc taagacacgg
     3301 atgcgattgg aaagaacctc actagtatat cacacgctct tctactttgc cgggtttcgc
     3361 caccattctc agagcaacta tgtcattgcg ccgcgccttg gtgtgcttga tcgtattatg
     3421 accgcatttg ccttccatca gcctctagag acatccgagt atctacgctc aggaatgccg
     3481 tcactgagac gctcgctcaa ttgatgagct aagcccattc atgaaagcta gccgatttga
     3541 acaaccatcc taagaatact gctactcgaa gaacaaacgg caatacggtt cgatttgctg
     3601 tcaaggttgc tgtattttgg gttatttcag tgtataagtg tgtgcgaggc tagttgtggg
     3661 gcggtttgat tgaatagagg gtttgaggta ggttatgccc ttatagcata tgacgcttga
     3721 ggattatgct ataatgtccc ctattagacg tatggaatac ttgtaggtaa gcgttactcg
     3781 catccgataa cgaactttac cgaccagagt caagagctgt catcctgagc gcagcgaagg
     3841 atctgtcggc tcatccaccg agatccttcg ctgcgctcag gatgacgttc tcttcctgat
     3901 attttttaga cgaaagccat tccatgaaaa aacaaaacgc cttccctgtc tctcgaccag
     3961 tgagcatcct gttactcgcc gcgctcgcgg ttctcatcat cgcctgctcc agcgccgcga
     4021 aacagggaac cagcagttcg agcaattcgt ccagctcgca gacctacaca tcaatcccca
     4081 tcgtcagtac tcctgagccg gagcagcccg cgcttccagg cgcggtcagc gtttacgtca
     4141 ccctccagga gtttacgatc acctcttcag tgcgcacctt ccacgccggg atacattact
     4201 acttcatcgt caccaatcgt ggccatgccg tgcatgagtt catgatgatg cgcgacaccg
     4261 ccaatggcac accaatttcc ttcgatgaag actccagcac aatgttcatt catatcgaac
     4321 aggtcttccc aggcacaaca ctgcacatga atttcacttt caccccctcc ctgatcggca
     4381 aatccgagat tgcctgcctg atgcgcggcc attacgccgc cggaatgcac ctgccgattg
     4441 tggttgcagt ctgaacccaa ctcggaatgt catcctgagc gcagcgtcgg aatgtcatcc
     4501 tgagcgcagc gtcggaat
//
LOCUS       DASZAK010000677         7742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_657321, whole genome shotgun sequence.
ACCESSION   DASZAK010000677 DASZAK010000000
VERSION     DASZAK010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657321"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..479
                     /locus_tag="VGT44_11930"
     CDS             39..479
                     /locus_tag="VGT44_11930"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEV2581551.1"
                     /translation="MPAIVRELTIAAAPERVWDALTQPGEIGHWWTNDLNVTPEVGSF
                     AEFRFGEWGDFVLRFEVAELDQDKKVHWIHSLSPVAQWAGTSVTWQLTPVHNGTTLVF
                     THEGFVKKDEVYEQTRGNWNYFLASLKSYLEAGKGTPGAPPSFN"
     gene            complement(508..1422)
                     /locus_tag="VGT44_11935"
     CDS             complement(508..1422)
                     /locus_tag="VGT44_11935"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEV2581552.1"
                     /translation="MSPLYHSLAVDSTPDFLTIGHVTRDLLADGSFELGGTVSFAALT
                     TYRLGLVSAIATCADTAWFEFVPTRLPSIGLAGHVCSHTTTFVNRYSGEGFRTQYLRD
                     RADDLLVEDIPPAWKDAPIVLFGPLAQEFTPELVRSFPRGQGRILAATPQGWLRRWDD
                     DGRVWPTPWTESEEVLPLLDVLILSHDDLLPFADGSRTEADAILARWSMQVPLLVATD
                     GRHGATLFRNGVTERFPAYAASEVDPTGAGDVFAAAFLTYLSQHGDPRQAVDFANCVA
                     SLSIEKQGAAGIPTREEVDKRLELSGKP"
     gene            complement(1476..2417)
                     /gene="jag"
                     /locus_tag="VGT44_11940"
     CDS             complement(1476..2417)
                     /gene="jag"
                     /locus_tag="VGT44_11940"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEV2581553.1"
                     /translation="MESIEASGKSVEDAILQGLARLKRRRDEVEITVLQEPARGAFGI
                     GNKDARVRVAVRLNSAGAIITPEMADALLGPDEEEEYADEELYDEEEFEDEEDEEDEE
                     FEDEEFEEGEEFDEDEFAPVEGGAASLAPGFLPTPGELNDLGDISVAEGEMQDVENPS
                     NEDVNITVDVLRHILQYMNIRAIVQVRSSNPLMLNIQGINENLGLLIGRRGETLAALQ
                     LLVSLIVGHRTRHRMRIIIDAENYRERREQNLRSLALRVAQQVRNYRRSIALEAMPPH
                     ERRIVHIALADSKDISTESIGEGEERRVVISLKRPGR"
     gene            complement(2477..3508)
                     /locus_tag="VGT44_11945"
     CDS             complement(2477..3508)
                     /locus_tag="VGT44_11945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEV2581554.1"
                     /translation="MSQIGYFFNIIFTFPIFNLLMLLHYVIGDFALSIIVLTVIMRMA
                     LFPLTLKQLKSTKAMQQIQPLIADVKKQYPKDQRAQLEATQAIYKEYGINPAAGCLPL
                     LVQLPVLYGLFYALNTVLRGGTIATINNTIYPFLPKLPLTTNITALTDFHWFAFLNPA
                     WHMPLSASDPTHILPVLAGLATFVQLRMSQPRNVSTSNNAMANQMKIMQYIMPFFTFF
                     IALNFPAGLALYWTTSSLFSMVQQYFVTGWGSLLERPKFFDSLGLGGTSKASSSNGKS
                     NNGDSRKETRLLSETADSNAAAVEGVGGRNGSNGAYNGNSSRRRPPRPGSASARRRGN
                     TPKRNPSRS"
     gene            complement(3505..3759)
                     /gene="yidD"
                     /locus_tag="VGT44_11950"
     CDS             complement(3505..3759)
                     /gene="yidD"
                     /locus_tag="VGT44_11950"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV2581555.1"
                     /translation="MKYVALFLIRLYQHTLSVILPPSCRFTPSCSHYGYEAIERFGFF
                     RGGWLTLKRIGRCHPFYHGNLYDPVPEAWLTRSRKTGDIS"
     gene            complement(3793..4161)
                     /gene="rnpA"
                     /locus_tag="VGT44_11955"
     CDS             complement(3793..4161)
                     /gene="rnpA"
                     /locus_tag="VGT44_11955"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV2581556.1"
                     /translation="MALSRVWRLRNNSDFQRVRQQGQNLSSRLLILAWNPNEVAQLRV
                     GFVVSKRISKRAVQRNYIKRLLGEAVRGVLPALPANIDIVFTARGQVAATDLSALRQD
                     MLALLRRAKLLSPGITLQEA"
     gene            complement(4201..4338)
                     /gene="rpmH"
                     /locus_tag="VGT44_11960"
     CDS             complement(4201..4338)
                     /gene="rpmH"
                     /locus_tag="VGT44_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV2581557.1"
                     /translation="MKRTWQPKRIPRKREHGFMKRMSTRNGRRVLKARRAKGRWKLTV
                     V"
     gene            complement(4420..5046)
                     /locus_tag="VGT44_11965"
     CDS             complement(4420..5046)
                     /locus_tag="VGT44_11965"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2581558.1"
                     /translation="MAVYCSQGHENSDGSAFCDTCGEELTSAAPVAAAPADAGTQVCP
                     KCGTVSSASETFCSNCGTLLGSAAAPVDSASSSVGSAPVVAAAPVAAAAPAVAAAPAL
                     TARLLVEADSQEFDLSGKDIVVIGREDAVSNIYPDVDLTPHGGEEGGVSRMHARIFIE
                     NGQYMLEDENSTNFTFLNRQKLTPKTPTALHDDDEIRLGRVLLRFKEA"
     gene            complement(5084..6322)
                     /locus_tag="VGT44_11970"
     CDS             complement(5084..6322)
                     /locus_tag="VGT44_11970"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2581559.1"
                     /translation="MPGEVTLTHEVGKDFMPVTGGSQLAYVLIEAKPTEMMAQVRMPL
                     NFALVLDHSGSMRGAKLRNVREAVKMVIDRLEPTDYVSVVIFDDSSQVIIPSMPANDK
                     IGMKAAIDQIRDAGGTTMSLGMINGLNELRRWSIPHAVTRMILLTDGVTYGDSDRCIR
                     LAGDAAAAGIAIYPLGIGSDWDEDLLDKIGRLSGGMPAEFIRDPADALAVFEQQIQSA
                     VDVAVRNASLIMRLPVGVSPRKVVKVLPIISEYDQSVMSDRQIVLPLGDLEKDKPQSV
                     LVELMIDPRPEGLFRIGQTELSYDVPMVGATSERTRDDIKVTFSHDANQTAAVNATVM
                     NFAEKANAHRLVTRVLDEYKRTGKATTRLAPNVTRVLDEETQAALEQINQGQQISQEQ
                     VKSIGNKTRKLTQRLDDLLP"
     gene            complement(6400..>7742)
                     /locus_tag="VGT44_11975"
     CDS             complement(6400..>7742)
                     /locus_tag="VGT44_11975"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEV2581560.1"
                     /translation="NGGYPEVEANDMTSNTATPGDGAPTGQAEQVETPEADTTAEADL
                     AGETGEGGATTQTDADGQQEQDFPVLAVGTIVMGRYEVTELISDEAHEHVYHVVDHQG
                     YQRCWSCGFEQNGEGDEFCGECGASLLNAPYIMHEYSAQESAGDAPVMQGNIVNTFVD
                     QGRTYAIEQPQAEQSAFPNGVCLESATASDAGNVRRSDPNEDSTLVMHFQRIHESISS
                     PVGAFIVADGMGGHDNGQGASRITINVIAERLARELLLAPLTAEKDGQPAAPFDEDAL
                     VLLLHGAVEDANNALCQKNAQEKSDMGSTLTGCMVVGEHAYIVNVGDSRTYMLRNKQL
                     YQVTTDHSLVAQLVASGLIEPDDVYTHPQRSQIFRSLGDKTNLQIDIFKQQLLPGDIL
                     LSCCDGLWEMVRNPQIESILNNAPDPQTACAQLIEAANTGGGEDNISAVVVFVR"
BASE COUNT         1615 a         2294 c         2143 g         1690 t
ORIGIN      
        1 tgctcttctc atgactgcct taaaaggaag gaacaacaat gccagccatt gtcagagaac
       61 tgaccattgc cgcagccccg gagcgcgtct gggacgccct gacgcagcct ggcgaaatag
      121 gtcactggtg gaccaacgat ctgaatgtca caccagaggt gggttctttt gctgagtttc
      181 gctttgggga gtggggggat tttgtcctcc ggtttgaggt ggccgaactg gaccaggaca
      241 aaaaagtaca ctggattcac agcctcagcc ccgtggcgca atgggccggg accagcgtca
      301 cctggcaact cacgccggtc cacaacggga caacgttggt cttcacgcat gaggggttcg
      361 taaagaaaga tgaggtttat gaacagacac gcgggaattg gaactacttc ctggccagcc
      421 tgaagtccta cctcgaggca ggcaaaggta ctcccggggc ccctccttcc ttcaactaag
      481 gttgttttcc ctctgcgagg tgtctcctta aggcttgccg ctcaactcca accgcttatc
      541 gacttcctct ctcgtcggaa tcccggcagc gccctgtttt tcgatagaga gcgaagcgac
      601 gcaattggcg aagtctaccg cctggcgtgg atcgccgtgc tgggagagat atgtaagaaa
      661 agccgcggcg aagacatcgc ccgcgccggt gggatcaact tcgctggcag cataggcagg
      721 gaagcgttcg gtgacgccgt tgcgaaagag cgtcgcgcca tgccgcccat cggtagcgac
      781 cagcagggga acttgcatac tccagcgtgc caggatagca tcggcctcgg tgcggctgcc
      841 atcggcgaag gggagcaggt catcgtggct gagaatcagc acgtccagca ggggcaggac
      901 ctcttctgac tccgtccagg gtgttggcca gacgcggcca tcgtcgtccc agcggcgcag
      961 ccagccctgg ggggtggccg ccaggatgcg cccctggccg cgcgggaaac tccgcaccag
     1021 ttcgggcgta aattcctggg caagcggacc aaacaggaca attggcgcgt ctttccaggc
     1081 cggcgggata tcctccacga gcagatcatc ggcccggtcg cgcaggtatt gggtgcgaaa
     1141 tccctcgccg gaatagcggt tgacgaaggt ggtagtgtgc gagcagacat gcccggccag
     1201 gccaatagaa ggcaggcgtg taggcacgaa ttcgaaccat gcggtatcgg cgcaggtggc
     1261 aatcgccgac accaatccta aacgataggt tgtgagggcg gcaaaagata cggtgccgcc
     1321 cagctcaaat gacccatccg caagcaaatc gcgggtaaca tgaccaatcg ttaggaaatc
     1381 cggggttgaa tcaacggcaa gcgagtggta gagcggtgac atgccacctc catattcata
     1441 aaccatcgat gcaccccggt ggggatgcgc tctatttagc gcccaggtcg tttcagggaa
     1501 atcacgacac ggcgctcttc gccctcaccg atgctttcgg tgctgatatc cttgctatct
     1561 gccagggcga tatgcacgat gcggcgctcg tgcggtggca tagcctcgag cgcgatcgag
     1621 cgtcgataat tgcgcacttg ctgcgcgacg cgcaaggcca atgagcgcag gttctgttcg
     1681 cggcgctcgc gataattttc cgcgtcgatg ataatacgca tgcggtgccg cgtgcgatgc
     1741 cccacgatca ggctgaccag caactgcagc gctgccaggg tctcaccgcg ccgcccgatc
     1801 agcagaccca ggttctcatt gatgccctga atgttgagca tcagaggatt ggatgagcgg
     1861 acctggacaa tggcccggat gttcatatat tgcaaaatat ggcgcaggac atcaacggtg
     1921 atattgacat cttcattact cggattctcg acatcctgca tctcgccttc cgccacgctt
     1981 atatcgccaa gatcgttcaa ttctccggga gtgggcagga accccggagc cagggacgca
     2041 gcgcctccct ctaccggagc gaactcatcc tcatcgaatt cttcgccttc ctcaaattcc
     2101 tcatcctcga actcttcatc ctcttcatcc tcttcgtcct caaattcttc ctcgtcgtat
     2161 aattcctcgt cggcgtactc ttcttcttcg tctgggccga gcagcgcgtc tgccatctct
     2221 ggcgtaatga tcgcgccagc gctgttcaaa cgcaccgcga cacgcacacg cgcatccttg
     2281 ttgccaatgc cgaaagcgcc gcgcgccggc tcctgcaaca cagtaatctc cacctcgtca
     2341 cggcggcgtt tcaaacgcgc gagcccctgt aaaatcgcat cctcaacact tttgccactg
     2401 gcctctatac tttccacagt ttgtagcctc cctttatttc tatgacggcg cgcgaacgtg
     2461 gagccgtctg actatgctag ctgcggctgg gattgcgctt aggtgtattg cctcgccgac
     2521 gggctgaagc gcttccgggg cgcggcgggc gacggcgcga gctattccca ttataggctc
     2581 cgtttgaacc attgcggccg ccgacccctt cgaccgccgc tgcgtttgaa tcggctgttt
     2641 cagacaaaag tctggtttct ttgcgcgaat caccgttgtt ggatttgcca ttactgctgc
     2701 tcgccttgct cgtccctccc aaccccaggc tatcaaaaaa ctttggcctt tccagcagcg
     2761 acccccaacc ggtcacaaag tactgctgca ccatactgaa gagtgaagag gttgtccagt
     2821 acaacgccag tccagccggg aaattcaggg caatgaagaa ggtgaagaac ggcataatat
     2881 actgcatgat cttcatctgg ttggccatgg cgttattcga ggtgctgacg ttgcgcggct
     2941 gcgacatgcg cagttgcaca aaggtagcca ggcccgccag gaccggtagg atgtgagtcg
     3001 ggtcagacgc gctcagcggc atatgccagg cgggattgag aaaggcgaac cagtgaaaat
     3061 cggtcagagc ggtgatgttt gtcgttaacg gcaacttggg cagaaaggga tagatcgtat
     3121 tgttgattgt ggcaatcgtt ccgcctctca acaccgtatt caaggcataa aacaggccgt
     3181 acaacacagg caactgcacc agcagaggca aacatccggc ggcgggattg atgccgtatt
     3241 ctttgtagat ggcctgcgtt gcctcgagct gcgcgcgctg atctttgggg tactgtttct
     3301 tcacgtcggc gatcaacggc tgaatctgct gcatagcctt ggtggacttc aattgcttca
     3361 aggtgagcgg gaacagcgcc atgcgcatga taacggtcag cacaatgatc gacagggcga
     3421 aatcgccgat cacatagtgc agcagcatca acaggttaaa gatggggaac gtgaagatga
     3481 tattaaaaaa gtagcctatc tgactcacga gatatctcct gtcttcctcg atctagtcag
     3541 ccaggcttct gggactggat catagagatt gccatgataa aagggatggc accggcctat
     3601 gcgtttcagg gtcaaccacc ctccccggaa aaagccaaaa cgctcaatgg cctcgtatcc
     3661 atagtgcgag cacgagggcg taaagcgaca tgacggaggg agaatcaccg acagggtatg
     3721 ctgatataga cgaatgagaa aaagcgcgac gtatttcact gtcgatgtcc tttggtttcc
     3781 tggttgggtg tgctacgctt cctggagagt tattccaggc gagagcaact ttgcccgtcg
     3841 tagcagagcc agcatatctt gcctcaacgc gctcagatca gtggcggcaa cctgccccct
     3901 cgccgtaaac acaatatcta tgttcgcggg taacgcgggc agaacacctc gaaccgcttc
     3961 accgagcagc cgttttatat aattacgctg caccgcacgc tttgagatgc gtttgctcac
     4021 cacaaatcca acacgcagtt gcgccacctc attcggattc caggcgagga taagcagccg
     4081 ggacgagaga ttctgcccct gctgcctgac tcgctgaaaa tcgctgttgt tgcgcagccg
     4141 ccatacacgt gagagcgcca ctgttgttta acagtattct aacgattgac acaatacaaa
     4201 ctaaacgacg gtcaatttcc agcgcccttt ggcgcggcgg gccttgagga cacgtcgccc
     4261 attgcgggtg gacatgcgct tcatgaaccc atgttcacgt ttgcgcggaa tgcgcttggg
     4321 ttgccaggta cgtttcatga taccctttgc tcctccacgt taagatttcc tgtagattat
     4381 agcggtttcc cctataaact gtcaattgtg catgaccctc tatgcttcct taaagcgcaa
     4441 gaggacgcgg cccagcctga tttcatcgtc atcatgcaag gcagtcggcg ttttgggtgt
     4501 aagcttctgc cggttgagga acgtgaaatt ggtgctgttc tcgtcctcaa gcatatactg
     4561 gccattctcg ataaagatgc gcgcgtgcat acgtgagacg ccgccctctt cgccgccgtg
     4621 cggcgtcagg tcaacatccg ggtaaatgtt gctcacggca tcttcgcgcc cgatcaccac
     4681 gatatctttg ccactcaggt caaattcctg gctgtcggcc tccaccagca gccgagccgt
     4741 cagggcgggc gctgcggcca cagccggggc cgctgcggca accggcgctg cggccacaac
     4801 tggcgcagag ccgacgctcg acgaggcgct atctaccgga gcagcagcgc tgccaagcaa
     4861 tgtaccgcag ttggagcaaa acgtctcgct cgccgaactg accgtgccac actttgggca
     4921 aacctgcgta cccgcatccg cgggagcagc ggcgaccgga gctgcactcg tcaactcctc
     4981 gccacaggta tcacaaaaag cagaaccatc cgagttttca tgtccttgag aacaatagac
     5041 tgccatgatg ctctatcctc ctaaaaaact aagttccctg aatttatggc aacaggtcat
     5101 ccaggcgctg cgtcaacttg cgcgtcttat tgccaattga cttcacctgt tcctgtgaaa
     5161 tttgctgccc ctggttgatt tgctcaaggg cggcctgggt ctcctcgtcc aacacgcgcg
     5221 tcacgttggg cgccaggcgc gtcgtcgcct tgccagtgcg cttatattcg tccaacacgc
     5281 gcgtcacgag acgatgcgca ttcgcttttt ccgcgaagtt catcacggtg gcgttcaccg
     5341 ctgccgtctg gttggcatcg tgcgagaacg tgaccttgat gtcgtcgcgt gtgcgctcgc
     5401 tggttgcgcc caccatcggc acgtcgtagc tcagctccgt ttgtccgatg cggaagagtc
     5461 cctctgggcg cgggtcaatc atcaactcca ccagcaccga ctgcggcttg tctttttcca
     5521 ggtctcctaa aggtagaacg atctggcggt cagacatgac ggattggtcg tactcgctga
     5581 tgatggggag gactttgacg accttcctgg gtgagactcc taccggcagg cgcataatca
     5641 gggacgcatt gcgcaccgcg acatcgaccg cgctctggat ttgctgttca aagaccgcca
     5701 gggcatcggc ggggtcgcga atgaattcgg caggcatacc gccgctcaag cgtccgatct
     5761 tatccagcag gtcttcatcc caatcggagc cgatgccgag cgggtaaatc gcgattccgg
     5821 cagcagcagc atccccggcc aaccggatac aacgatccga gtcgccgtag gtgacgccat
     5881 cggtgagcag gatcatgcgc gtgacggcat gaggaatgct ccaacggcgc agttcgttga
     5941 ggccatttat catgccgagc gacatggtgg tgccacccgc gtcacggatc tggtcaatag
     6001 cggctttcat gccgatttta tcattcgcgg gcatcgaggg gataatcacc tgagacgagt
     6061 catcaaagat gacgaccgaa acatagtcgg tcggctcaag gcggtcaatc accatcttga
     6121 cggcctcgcg gacatttctt agtttggctc cccgcatgga gccagaatgg tcaagaacca
     6181 gggcgaagtt gagcggcatc ctgacctggg ccatcatctc cgtcggtttc gcctctatga
     6241 gtacatatgc aagctgactg ccaccggtca caggcataaa atcctttcca acctcgtgtg
     6301 tcagcgttac ctcgccgggc atagcataaa gctcctttcc ttatagcatg acgtagtata
     6361 tcatatcagt tagtatattc atacgtgcat cgtatcgctt tatcgcacaa agaccacgac
     6421 cgcgctgatg ttgtcctcgc ccccgccggt atttgcagcc tcaatcaact gcgcgcaggc
     6481 cgtctgagga tcgggtgcat tgttgagaat gctctcgatc tgcgggttgc gcaccatctc
     6541 ccacaggcca tcgcagcagc tgagcaggat atcccctggc agcaattgct gcttgaagat
     6601 gtcgatttgc aggttcgtct tgtctcccag gctgcggaaa atctggctgc gctgcggatg
     6661 cgtgtacacg tcatccggct cgatcaagcc gctcgccacc agctgcgcga ccagggagtg
     6721 gtcggtcgtg acttgataga gctgcttgtt gcgcagcata taggtccgac tatcgccgac
     6781 attgacgata taagcatgtt cgcccaccac catgcacccc gtcaacgtag accccatgtc
     6841 ggatttctcc tgggcgtttt tctgacagag agcgttgttc gcatcctcta ccgcgccatg
     6901 cagcaggaga accagcgcgt cctcatcgaa gggcgctgcg ggctggccat ctttctcggc
     6961 ggtaaggggc gcgagcagca attctcgcgc caggcgctca gcaatcacat tgatcgtaat
     7021 gcgactcgct ccctggccgt tatcatgccc gcccattcca tcggccacga taaaagcccc
     7081 caccggtgaa gagatcgact cgtgaatgcg ctggaagtgc atcaccagcg tgctatcctc
     7141 gttcgggtcg ctgcgccgca cattacccgc gtcggacgcg gtagcagact ccaggcaaac
     7201 gccgttgggg aacgcgctct gctcggcctg cggctgctcg atggcgtagg tgcgtccctg
     7261 gtcaacgaag gtgttgacga tgttgccttg catcaccggc gcatctcctg cactctcctg
     7321 cgcggaatat tcgtgcatga tatacggcgc gttgagcaag ctcgcgccgc actcgccgca
     7381 aaactcgtcg ccctcgccat tctgctcgaa gccacagctc cagcagcgtt gatagccctg
     7441 gtggtccaca acgtgataga catgttcgtg ggcttcatcg ctgataagct ctgtcacctc
     7501 gtagcgcccc atcacgatcg ttcctaccgc caggacagga aagtcctgct cttgctgccc
     7561 atccgcatcg gtttgtgtgg tcgccccgcc ttcgccagtt tcgcctgcca agtcagcttc
     7621 agccgtcgta tctgcctctg gcgtctctac ctgctcggcc tggccggtcg gcgcaccatc
     7681 acccggcgtc gctgtgttgg atgtcatatc gttcgcctcc acttcgggat agccaccatt
     7741 tt
//
LOCUS       DASZAK010000678         2275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_622578, whole genome shotgun sequence.
ACCESSION   DASZAK010000678 DASZAK010000000
VERSION     DASZAK010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622578"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2275)
                     /locus_tag="VGT44_11980"
     CDS             complement(<1..>2275)
                     /locus_tag="VGT44_11980"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV2581561.1"
                     /translation="AFNDTHRVVEYARITTDPASGALYLTFNALGASPQSPNSALAAT
                     WTSQLYFASSLNGGQAWSAPVAIGTGQQDYWGIPVVDWNGAILVFYSGALGLELVQSS
                     NGGQAWSSPRSLDPVNAVGEQRAYNEVYLNPGPGVAVDLSNKMLYVVYNGGQIILSSD
                     DGTTWSQPIDVLGNRYSPAFLASISVDPVSHIVSVGGYSSAGDPSQNTFAYYYAQSED
                     GGNHFSSPALVSDNASLPPPPAFGSIGRASSVVSGGQFARLFWTDTNGVNGNEQILTA
                     SVDLSQPLMGALQNSWDSAQLLPPWGVVFGGAGSQAIGVGDYGQGSIGAVSATPCAAC
                     SWLSASVSAGTAVGSWVVTVSTSSAATSQVGSVTIQATGLNSSITIPVQLQIDLATPA
                     FSLGTNALTFTTLQGIDPPTQAVQVDTLRSINEQLQVYTNGDPVLSASFANGSPNIVL
                     SPGVSNGLIVLVSVAGLPVGSSTHTLVVSDGTTSLDVTLTLTIDAAPAQLNAPASSLN
                     FAYHQQSALRPASQSIILRNSGGSALHWQGSTDVSWLSLGTTNGTIAPGALQQVMVNV
                     TTAELSQGTSIGHIVLGGDAQAVELPQIIPVYLVIDPPANQISKTWYFAEGYVSANFA
                     EYLTLENPNAQPASVAVTYLTQPINQAPKAPFTLTYQVNPGSRFTVSINSQPGIVQND
                     QVSLVVNASVPIVAERPMYFKYTVLNPNPTGGTDIVGATHLNSVFFFPFVQIGSDAQS
                     GSTTFGTTYSTYLTVLN"
BASE COUNT          463 a          675 c          711 g          426 t
ORIGIN      
        1 tggttcagca ccgtcagata cgtcgagtag gtcgtgccaa atgttgtaga gccgctctgt
       61 gcgtcgctgc caatctgcac gaagggaaag aaaaagaccg aattgagatg cgtcgcgccc
      121 acgatatccg tgccaccggt gggattcgga ttcagcacgg tatatttaaa gtacatcggg
      181 cgctccgcta caatcggtac gctggcattg accaccagcg aaacctggtc attttgtacg
      241 atgccaggct ggctgttgat cgacacggta aagcgactgc cagggttgac ctggtacgtc
      301 agcgtaaacg gcgctttggg agcctggttg atgggctgcg tcaggtaggt gacggccacg
      361 ctggccggct gcgcgttggg attttccagc gtgagatact cggcaaaatt ggccgaaacg
      421 tacccctcgg caaagtacca ggtctttgaa atctggttgg cgggtggatc gatgaccagg
      481 tagacaggaa tgatttgcgg cagctcaacg gcttgcgcat caccgcccag cacgatatgg
      541 ccgatgctcg tcccctgcga cagctcggcg gtcgtgacgt tcaccatgac ctgctgcaac
      601 gcgccgggag caatagtgcc gttcgtcgtt cccaggctca gccaggacac atccgtgctg
      661 ccctgccagt gcagcgcgct gccgccgcta ttgcgcagta tgatgctctg cgacgccggc
      721 cgcagcgcgc tctgctggtg ataggcgaag ttcagcgaag aagctggtgc attgagttgc
      781 gcgggcgcgg catcgatggt cagcgtaagc gtgacatcca gcgaagttgt gccatcgctg
      841 acgaccagtg tgtgcgtgct tgagccgaca ggcagaccag ccacgctgac aagcacgatc
      901 aggccatttg agacccccgg actcaacaca atattgggcg agccattggc aaaggacgcg
      961 ctcaggaccg ggtcgccgtt ggtgtaaacc tgcaattgct cgttgatcga gcgcagtgta
     1021 tcgacctgta cggcctgcgt cggcggatcg atgccttgca gcgtcgtaaa tgtgagcgcg
     1081 ttcgtgccaa gcgagaaggc aggcgtggca aggtcgatct gcaactgcac aggtatggta
     1141 atcgagctgt tcaggccggt cgcctggatg gtgacacttc cgacctggct ggtggccgcc
     1201 gagctggtcg agacagtcac aacccaactg cccaccgctg ttccggcgct gaccgaggcc
     1261 gaaagccagg aacaggctgc gcacggtgtc gcgctgacag cgccgatgct gccttgccca
     1321 tagtcgccca cgccgattgc ctggctgccc gcgccgccga agacgactcc ccagggtggc
     1381 agcaactgcg cagaatccca actgttctgc agcgcgccca tcaacggctg gctgagatcg
     1441 accgatgccg tcagaatctg ctcgttgccg ttgacgccgt tggtatccgt ccagaacagg
     1501 cgcgcgaact ggccgccgct cacgacactg ctcgcgcggc caatcgagcc gaaggctggc
     1561 gggggcggca gcgaggcatt atcgctcacc agtgccgggc tggagaaatg gttgccgcca
     1621 tcctcggatt gcgcgtagta ataggcaaag gtattctggg aggggtctcc ggcgctggaa
     1681 taaccaccca cgctgacgat atgcgagacg ggatcgacgc tgatcgaggc cagaaacgcc
     1741 ggtgaataac ggttgcccag cacatcgata ggctggctcc aggtcgtgcc atcatcgctt
     1801 gaaaggatga tctgaccgcc attgtacacc acgtagagca tcttattgct caaatcaacg
     1861 gcaacgcccg gtccagggtt gagatacact tcattataag cgcgctgctc cccgacagca
     1921 ttgaccggat cgagactgcg cgggctgctc cacgcctgcc cgccattgct ggattgcacc
     1981 agctccaatc caagcgcgcc gctgtaaaac accaaaatcg cgccgttcca atccacgacg
     2041 ggtatgcccc agtaatcctg ctggcccgtg ccgatggcga cgggcgcgct ccacgcctgt
     2101 ccgccattga gcgagctggc gaaatacaac tgcgatgtcc aggtcgccgc gagcgcggaa
     2161 ttgggggact gcggcgacgc gcccagggca ttgaaggtga gatagagcgc gccacttgcc
     2221 gggtccgttg tgatgcgcgc atactcgacg acgcggtgag tatcattgaa cgccg
//
LOCUS       DASZAK010000679         2936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_17383, whole genome shotgun sequence.
ACCESSION   DASZAK010000679 DASZAK010000000
VERSION     DASZAK010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2936
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17383"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1885)
                     /locus_tag="VGT44_11985"
     CDS             complement(<1..1885)
                     /locus_tag="VGT44_11985"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEV2581562.1"
                     /translation="MGETLNVQFHRREDSAFEVQVKESWSGHSVRGDFVPPYSSRQLG
                     SLLKKLNTQESSERELVDVGHRLFLALCGADAVIPGGKPGSRSGLATNGALVRGSAEH
                     SVQAMLRSVIQRTLRRRGTVALTLIFEEGCEEFVSYPWELLHNGEHFLLVAGVFTLTR
                     ALLLPDQPAQTELPIYPPLRLLYIGAAPVDLPPLELETSFESLQRGLLPLMEANQLKL
                     DRLEPPTFDELVRYLNSYGGAGAFNDRDTALPCYAVHFDGHGAFGRLCPADDCDEMNE
                     INARVCKACKRSLGKAKPQTYLCFCDDRGRNRYISTESLRELFVSTDVRLAVFSACDT
                     AHYIEEKDRQQRRAAVDASLATALVMAQVPAVVAMPFSIQDDLSPTFMYHFYDTIAAG
                     RTLEEALARSRQAMLPVKVPSWFVPVLYRHVVEGQIGPVALLAGRDDPQTYDHPLLHL
                     RPTGVFVGREREIDELSLFLEQAAQNDNRKSRPATHHLALTGPTGIGKSELAFEVARR
                     NTEKFSGGITAISLQDGKTLGDALIEMAHTLHIHSKSMNTADVPQCERTVLNAYRGLA
                     NRDMASLLLLDSFEEVEGSADAGHWCRFLCALPERVIVLITSRSNPATFAALEGATCR
                     WYEY"
     gene            complement(1894..>2936)
                     /gene="obgE"
                     /locus_tag="VGT44_11990"
     CDS             complement(1894..>2936)
                     /gene="obgE"
                     /locus_tag="VGT44_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV2581563.1"
                     /translation="KAPAGTIARDAETGELLADLTESGQRVLVARGGRGGLGNVHFAT
                     ATHQAPHEAQNGEPGEECWVLLELQLIADVGLIGYPNAGKSTLLSVVTAAKPKIADYP
                     FTTLEPNLGVVAVGQPGSGDEFGFVLADIPGLIEGAAQGVGLGHEFLRHVQRTRLLLH
                     ILDGASPERHPWQDFLNINKELREYDERLATRPQIIVFNKMDLPEAREAWPALKAKAE
                     EQGYTVFAISAAAHQGTGELMAFAANRLQEIQQEEAERSTQLAQQEEGVVLRPQPDDA
                     FTVAREKDYFIVRGRRVERTVSMTNLGNDESMDRLQITLAKMGVTKALEEAGVKVGDR
                     VRFGKVELYWGE"
BASE COUNT          543 a          914 c          890 g          589 t
ORIGIN      
        1 aatactcata ccagcggcag gtcgctccct ccagtgcggc aaacgtcgcg ggattggagc
       61 gcgaggtaat cagcacgatc acccgttctg gcagcgcgca caggaatcga caccagtgtc
      121 ccgcgtcagc gctgccctcg acctcctcaa agctgtcgag cagcagcagg gaggccatat
      181 cgcggttcgc cagcccccgg taggcattca gcacggtgcg ctcgcactgt ggcacgtcgg
      241 cggtgttcat cgatttggag tgaatatgca gcgtatgtgc catttcgatc agggcatcgc
      301 cgagcgtttt cccatcctgc aaagaaatag cggtgatgcc gccggaaaat ttctccgtgt
      361 tgcgccgcgc cacctcgaag gccagctcgc ttttgccgat tcctgtcggg ccggtcagcg
      421 ccaggtggtg cgttgctggc cgtgatttcc ggttatcgtt ctgagccgcc tgttcaagaa
      481 agagcgagag ttcatcgatc tcgcgctcgc gtccaacaaa gacgcccgtt gggcgcaaat
      541 gtaagagtgg atgatcgtat gtttgcggat catccctgcc ggccagcagc gccaccggcc
      601 cgatctggcc ctcgacgacg tgacgataga ggacaggcac aaaccagctc ggcaccttga
      661 ccggcagcat ggcctggcgc gaacgcgcca gagcctcttc cagtgtacgg ccagcggcga
      721 tggtgtcgta aaagtgatac atgaaggtcg ggctgagatc gtcctggatg gagaacggca
      781 tcgccacgac tgccggaacc tgtgccatca ccagcgccgt tgccaggctc gcatcgacgg
      841 cggcgcggcg ctgctggcga tccttttcct cgatatagtg cgcggtatcg caggcggaga
      901 aaacggccag tcgcacatcg gtgctgacaa acagctcgcg cagtgactcc gtgctgatat
      961 agcgattgcg cccccggtcg tcgcagaaac acaggtaggt ctgcggtttg gccttgccca
     1021 gcgaccgctt gcaggcttta cagacgcgcg cgttgatctc gttcatttcg tcgcagtcgt
     1081 ccgccgggca gaggcggcca aacgcgccat gtccgtcgaa atggaccgcg tagcatggca
     1141 gggcggtatc ccggtcattg aacgcgcccg cgccgccata agaattgagg tagcgcacca
     1201 gttcatcgaa ggtgggcggc tccaatctgt ccagcttgag ctggttcgcc tccattagtg
     1261 ggagcaggcc gcgctgcagg ctctcgaaac tggtttccaa ctccagtgga ggtaaatcaa
     1321 cgggcgcagc gccaatgtag agcaggcgca gcggtgggta gatgggcaac tcggtctgcg
     1381 ccggctgatc gggcaggagc agggcgcgtg tcagggtgaa gacgcccgca accagcagaa
     1441 aatgctcgcc gttgtgcagc aattcccagg gataactgac aaattcctcg cacccttcct
     1501 caaagataag tgtcaaggcg accgtgccgc gcctgcgcag ggtgcgctgt atgacgctgc
     1561 gcagcatggc ctgcacggaa tgttccgccg aaccgcgcac aagcgcccca tttgtggcaa
     1621 gccctgacct gctgcctggc ttgccaccgg gtatgacggc atccgcgccg cagagcgcca
     1681 gaaacaggcg atgtccgaca tcgacgagtt cgcgttcgct gctctcctga gtattgagct
     1741 tcttcagcag cgagcccagt tgcctgctac tatagggcgg gacaaagtcg ccacgcacac
     1801 tatgcccgga ccagctttct ttgacctgga cttcgaaggc gctatcttcc ctgcggtgaa
     1861 attgaacgtt gagtgtctct cccatgaata cggttactca ccccagtaca gttcgacctt
     1921 tccaaaacgg actcgatctc cgaccttgac gcctgcttct tccagcgcct tcgtgacgcc
     1981 cattttcgcc agggtaatct gcaggcgatc catactctca tcgttaccca ggttagtcat
     2041 actgacggtg cgctcgaccc gcctgccgcg cacgatgaag taatcttttt cgcgcgcaac
     2101 cgtaaaggcg tcatccggct gtgggcgcag tacgacgcct tcttcctgct gtgcgagttg
     2161 cgtagagcgt tccgcctcct cctgttggat ttcttgcagg cggttagcgg caaacgccat
     2221 cagttcgccc gttccctggt gcgcggcagc agagatggcg aagaccgtgt agccctgctc
     2281 ttctgccttt gctttaaggg caggccaggc ttcccgcgct tccggcaggt ccattttgtt
     2341 gaagacgatg atctgggggc gagtcgcaag gcgttcgtca tattcgcgca gttctttgtt
     2401 gatgttcagg aaatcctgcc agggatggcg ttccggggat gcgccatcca ggatatgcag
     2461 cagcaggcgg gtgcgctgca cgtgccgcag gaattcgtga cccaggccga cgccctgtgc
     2521 tgcgccttca atcaggccgg ggatgtcagc cagcacaaag ccaaattcgt cgccgctgcc
     2581 gggttgtcca acggccacaa cgcccaggtt cggttccagt gtcgtaaatg gatagtcggc
     2641 gatcttgggt ttcgccgccg tgacgacgga gagcaaggtc gatttgcccg cgttggggta
     2701 gccgatcagc cccacatcgg cgatgagttg caattcaagc agcacccagc attcctcgcc
     2761 cggctcgccg ttctgcgcct cgtgcggtgc ctggtgcgtc gcggtggcga aatgcacatt
     2821 gcccaggccg ccgcgcccgc cgcgcgccac cagcacccgc tggccagact cggtcaggtc
     2881 ggccaacaac tcgccggtct ccgcgtcgcg cgcgatcgtg cccgctggcg ccttga
//
LOCUS       DASZAK010000680         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_444539, whole genome shotgun sequence.
ACCESSION   DASZAK010000680 DASZAK010000000
VERSION     DASZAK010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444539"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VGT44_11995"
     CDS             <1..670
                     /locus_tag="VGT44_11995"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HEV2581564.1"
                     /translation="DEQLLFLARLNGLSKQAAEKELTEWLERFQISANRKKKIEELSK
                     GNQQKVQFLATILHDPTILVMDEPFSGLDPVNANVLKEAFLEMHRRGKTILFSTHQLE
                     QAEELCQDIVIINQGQSVVAGSVREVKRRQGRHVARLKLDNDPEASWLEQLQGVQVTR
                     RREDYIEMQLPASLNPNVIVEAALQHGGIISRFELTEPSLTDIFIELVGTSSLPDVPS
                     AASA"
     gene            687..2105
                     /locus_tag="VGT44_12000"
     CDS             687..2105
                     /locus_tag="VGT44_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2581565.1"
                     /translation="MATNTVGTPEKEVAEQEQGAVAVASGGAAAQHTLRNIRLIIGRE
                     YKNRVTQRSFVITSVILLVIVFLAPFIPTIVQLVQHGTAQTASQIQVVVVNNAGTVAG
                     LSEAGLLSSIGSQLNGTNPPSPAPYAISGQPPTALDGLRNQVKQGTLDILLVLDRSPQ
                     GNLQLLYDANTDPNNDPNQSTIQTLALDLTFFDTAHRLGLTSQETQRLATPPDLIVMH
                     TRQSQTTQPTSVSPADYVLAVAGVILIFISIQIYASIVANGVAEEKSSRIMEILVNAA
                     TPNQLLAGKIVGIGAACLTQMGCMVAVGIAALLLQAPLQAALFGASTAGFSQYLTAAS
                     IPFYLLFLLYFLLAFFLYATLFAGLASLVKRQEEVQSVIILPLLLLSGGYVLFFFTVA
                     TPDATLTKVLSYIPFWTPIFMLMRLAVGTVAWWEIVITVALMLVTILACTWFAARLYR
                     YGILMYGQRPSIGQIVKLARMN"
     gene            complement(2215..>2545)
                     /locus_tag="VGT44_12005"
     CDS             complement(2215..>2545)
                     /locus_tag="VGT44_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV2581566.1"
                     /translation="QLARTGPYIPQAPLAIVVAMDHNRSIAVSDGSRAIQDMILAAWS
                     QGVGSNWVGFNSLPEVNPLLGIPEEVSVLAIVPFGYPAEAVGKGQKKRKPLGEVAYNE
                     RWGEPFK"
BASE COUNT          538 a          758 c          710 g          539 t
ORIGIN      
        1 ggatgagcag ctcctgttcc tggcgcggct gaacggttta tcaaagcaag ccgccgagaa
       61 agagttgact gagtggctcg aacgttttca gatcagcgcc aatcgcaaaa agaagatcga
      121 agaactctct aaaggtaatc agcagaaggt gcagtttctg gcgaccatcc tgcatgaccc
      181 aacgattctg gttatggatg aaccgtttag tggcctggac ccggtcaacg cgaacgtgct
      241 gaaagaggcg tttttagaga tgcatcgccg gggtaaaacc attctcttct ccacgcacca
      301 gttggagcaa gcagaggaat tatgccagga tatcgtgatt atcaatcaag ggcagagcgt
      361 cgtcgccggc agcgtgcgcg aggtcaaacg acggcaaggg cgccatgtgg cacggctcaa
      421 actcgacaac gatcccgagg cgagctggct tgagcagctt caaggcgtgc aggtcaccag
      481 gcgtcgggag gattacatcg aaatgcagct cccggccagt ctcaacccca acgtgattgt
      541 cgaagccgcg ctacaacacg gcggcatcat cagccgtttc gagctgaccg agccatcact
      601 gaccgacatc ttcattgaac tggtggggac gagttccctg ccagatgttc catctgccgc
      661 ctcagcatag aaaaggaaaa gaaaccatgg caacgaacac tgtgggaacg ccagaaaagg
      721 aagtcgcgga acaagagcaa ggggccgtcg cggtcgcttc gggcggcgct gctgcccaac
      781 acaccctgcg caatatccgt ttgatcatcg ggcgcgagta caaaaaccgg gtgacgcagc
      841 gcagctttgt catcaccagc gtgatcctcc tggtgatcgt gttcctggcc ccttttatcc
      901 cgacgatcgt gcagctggtg cagcatggta ctgcgcagac agcttcgcaa atccaggtcg
      961 tggtggtgaa taacgcgggg acggttgcgg gcctctctga ggcggggctg ctctcgtcca
     1021 tcggttcgca attgaacggg acgaatcccc ccagcccggc tccgtatgcg attagcggcc
     1081 aaccaccaac tgcccttgac ggcctgcgaa accaggtcaa gcagggcaca ttggatatcc
     1141 tgctcgtgct cgatcgttcc ccgcaaggga acctccagct tctctatgac gccaataccg
     1201 atcccaacaa cgaccccaac cagtccacga tccagacgct ggctctggat ctcacctttt
     1261 tcgataccgc tcatcgcctg ggcctcacct cgcaagagac ccagcgcctc gccactcccc
     1321 ctgacttgat cgtcatgcat acccggcaga gccagacgac tcagccgacg agcgtaagtc
     1381 ctgcagacta tgtgctcgcc gttgccgggg ttatcctaat cttcatctct atccagatct
     1441 atgcctcgat cgtggcgaat ggagtggcgg aagagaaaag cagtcgtata atggaaatcc
     1501 tggtgaatgc cgcgacccca aaccagctgc tggccggcaa gatcgtgggc atcggggcgg
     1561 cgtgcctgac gcagatgggg tgcatggtgg cggtcggcat cgctgcgctc ctgctgcaag
     1621 cccctctgca agcagcgctg ttcggggctt ccacggcagg cttcagccag tacctcactg
     1681 ctgcgtccat cccgttctac ctcctgttcc tgctctactt tctgctggcg ttcttcctgt
     1741 atgccaccct gtttgccgga ctggcctcac tggttaaacg gcaggaggag gtacaaagcg
     1801 tgatcatact tcccctgctg ctgctcagcg gtggctatgt gctgttcttt ttcacagtcg
     1861 ctactcccga tgcgacattg acgaaggtgc tgtcgtatat ccctttctgg accccgatat
     1921 tcatgttgat gcgtcttgct gtgggtacgg tcgcctggtg ggagatcgtc ataacggtcg
     1981 ccttgatgct ggtgaccatt ctggcctgca cctggtttgc cgcgcgcctg tatcgctacg
     2041 ggatcctgat gtacgggcag cgccccagca taggtcagat agtgaaactg gcacggatga
     2101 actaaccgcc ggtgaacgac gggatctctc gaaagaggaa cttcgttgct tcagtgtctt
     2161 agcagacatg ccttatccga agttcctcat ctcctcttgg tgctcatgca cctcttattt
     2221 gaacggttct ccccatcgtt cattgtaggc aacctcgccc agtggcttgc gtttcttctg
     2281 acctttcccg acggcctcag ccgggtagcc gaacggaacg atcgcgagca ccgaaacctc
     2341 ctcaggaatg cccaatagcg ggttgacctc cggcaagctg ttgaagccaa cccagtttga
     2401 gccgacgcct tgcgaccatg cggccaggat catgtcctgg atagcgcgac tgccatcgga
     2461 gacagcaata gagcggttgt gatccatagc caccacaatc gccagcggcg cctgcgggat
     2521 atagggaccc gtgcgtgcga gctgc
//
LOCUS       DASZAK010000681         3891 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_17424, whole genome shotgun sequence.
ACCESSION   DASZAK010000681 DASZAK010000000
VERSION     DASZAK010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17424"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..438)
                     /locus_tag="VGT44_12010"
     CDS             complement(16..438)
                     /locus_tag="VGT44_12010"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEV2581567.1"
                     /translation="MFKTERNLDKAMTSEAIARLENTAFAMQAMQEGYPQIALLFMEA
                     AGAETIHGINHMRAAERIGSTFENLDKAANGEDFEIEQMYPTFLREAVVEGHEDAATS
                     FHLALEREKQHRAMFKEAFDEFKAGLIEKEYMPSQTPS"
     gene            complement(627..1622)
                     /locus_tag="VGT44_12015"
     CDS             complement(627..1622)
                     /locus_tag="VGT44_12015"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEV2581568.1"
                     /translation="MPTKYHFCNSLSWESGQDVSTRFGPQQTGGSIIQHVRPVDLQAS
                     MAQGAEILARWAGILAETGIEIAGITFADEIATWQQVANDYTARTRLMWRNGWFRDYD
                     SVAKEWSAEQDAMHLAPVFCGVADWEQIERLRPALAEPPSNNPYWRPLSWPPIIMTVA
                     EAASVAGMPAEAAELAYRFIDASYRSIDSRTPDEYGNIPGITRENRRLVTSGKWGATD
                     YVNSGIEGYGWGAVSVFLLLRHLLGLHEEKADVIKVAPVFPQALRRVGATYHAGPVPW
                     GKYLLHFQCIVRDSSSYSMQMRCAVQTAPEAPQGVVEHQWAWEGAWGDERVVHLP"
     gene            complement(1672..>3891)
                     /locus_tag="VGT44_12020"
     CDS             complement(1672..>3891)
                     /locus_tag="VGT44_12020"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HEV2581569.1"
                     /translation="SYVNNQFQYSAHLTIGLNINDLVLVTQEDFLRHRCTINSQLHTK
                     NRFEYTCIVSQYELTLTIRYFLVQEHVLGCTLTLATTFPAPVPITCYLIHRHVHNPHT
                     SRLWEHGLYALQDRQTGCGMLGLASEGDVFVHGARLSDNQDIANGPALTSDSMGFATS
                     LDELSAWARGVRIPWPRITQRQPDTGWQHRALAMPYRLILGGSSPSSITLHAALARGV
                     SQDKALFYWLEGMDSINEAESMHRENDESFWQQAPKLTGDWPAHWQRGFVYDFETLRM
                     VTRPPASAIPHVWDGMQIQAPRTVLAEAAMDTLFLSYADPELAAEVILGHFESAPHPN
                     LPCMREDGSFNMVADDGQICGTAPEWGYPLWCCQQLWLRSGDLSWLKRLYPGAAAYLR
                     WWLDHRRDSEGWEIYGCSWESGQDVSIRFGPQQTGGTIIQHLRPVDLQASIAQGAAIL
                     ASWAEILSSTHADETLPNPYTADIAFWQEVANEFTVKTRLMWQNGWFRDYDSVRGIWS
                     SEQDAMHLAPVFCGVAEREHIEQLRPALAQPPSNNPHWLPLSWPPIIMTVAEAARLAG
                     MSAEAVELAYRFVDASYRSADNRTLDEYGNIPGVTRENRRAIKVGKWGEIDYVNAGIE
                     GYGWGAVSIHLLIRHLLGMYATGLNSITLAPTLPLALRRPGATYTIAPIPWGKYLLSL
                     TCRVKSAHSYEATFHVRPRAQAAQPAGETEARSETAGEQEHHWEGAWGEECTFLLDH"
BASE COUNT          779 a         1137 c         1156 g          819 t
ORIGIN      
        1 tacccacgcg ctggctcatg agggggtctg cgagggcatg tattccttct cgattaagcc
       61 tgccttaaac tcatcgaagg cttctttgaa catagcgcgg tgttgtttct ctcgttctaa
      121 ggcgaggtgg aagcttgttg cagcatcctc gtgaccctcc accaccgcct cgcgaagaaa
      181 ggttggatac atctgttcga tctcgaagtc ttccccattc gcggctttgt ccaggttctc
      241 aaaggtcgat ccgatccgtt cggcggcacg catgtggttg atgccgtgga tcgtctcggc
      301 gccggctgcc tccatgaaca acagggcaat ttgcgggtag ccctcttgca tggcctgcat
      361 tgcaaaggcc gtattctcca gacgggcgat cgcttcgctg gtcatcgcct tatccaggtt
      421 acgttcagtc ttgaacatga catctctcct tgcgcttgtg acatcggaac gggaacgcca
      481 tcgcagccga aagcgcccgc aatgactctc catctgccac cataagcata ggccagagca
      541 ttctcacgaa ggtcacagtc atgagtctca atgacacgaa ctggtcacaa gtgcgaggag
      601 ggctgtctcg aacctatagg agatttctac gggagatgaa caactctctc atctccccat
      661 gctccttccc acgcccattg gtgctctacc actccttgag gcgcttcagg agcagtttgc
      721 acagcgcagc gcatctgcat gctgtaactg ctggaatctc gcacaataca ctggaaatgc
      781 aacagatact ttccccatgg caccggcccc gcgtgatagg tcgcaccaac ccgccgcagc
      841 gcttgcggga atacaggagc aaccttgatc acgtccgctt tttcctcgtg tagtcccagg
      901 agatggcgca agagcaggaa gacgctgacg gctccccagc catagccttc gatgcccgag
      961 ttgacgtagt cggtcgcacc ccacttgccg gatgtgacca ggcggcggtt ttcgcgggtg
     1021 atgcccggaa tgttgccata ttcgtcgggg gtgcggctgt cgatgctgcg gtaagaggcg
     1081 tcgatgaagc ggtaggccag ttcggcggcc tcggcgggca tcccggcgac actggccgct
     1141 tcggcgacgg tcatgataat aggcggccag ctcagaggtc gccaatatgg gttgttgctt
     1201 ggtggctccg ccagggccgg gcgaagccgt tcgatctgct cccagtcggc cacgccgcag
     1261 aagacagggg caaggtgcat ggcatcctgc tcggctgacc actccttcgc cacagagtcg
     1321 taatcgcgga accagccatt ccgccacatc aggcgggtcc tggcggtgta atcattggcg
     1381 acctgctgcc aggtcgctat ttcgtccgcg aaagtgatgc ctgctatctc gatgccagtc
     1441 tccgccagta tcccggccca gcgcgccagg atttccgcgc cctgcgccat gctggcctgc
     1501 aagtccaccg gtcgcacgtg ctggatgatt gagccgccgg tctgttgcgg gccgaagcga
     1561 gtggagacgt cttggccgct ttcccaggag agtgagttgc aaaagtggta ctttgtcggc
     1621 atcacgcatg caggctatta cattaatccc aggctgttgg aaggcgcacc gtcagtgatc
     1681 cagtagaaac gtgcactctt cgccccaggc tccttcccag tggtgttcct gctcgccggc
     1741 tgtctctgac cgggcctcgg tctcccccgc tggctgagct gcttgtgcgc gtgggcgcac
     1801 atggaaggtt gcttcatagc tatgcgcgct tttcacccgg caggtgagac tcaacaggta
     1861 cttcccccag ggaatgggag caatggtata cgttgcgcca gggcggcgca gcgccagggg
     1921 gagggtcgga gccagggtga tgctattgag gccggtggca tacatgccca gcagatggcg
     1981 tatgagcaaa tgaatgctga ctgcgcccca gccgtagccc tcgatccccg cattcacgta
     2041 gtcgatctcg ccccatttgc ccaccttgat agcgcggcgg ttctcgcgcg tgacccccgg
     2101 tatattgcca tactcgtcca gcgtgcggtt atcggcgctg cggtaggagg catcgacgaa
     2161 acggtaggcc agttccacgg cttcggcgga cataccagcg aggcgggccg cttcagcgac
     2221 ggtcatgatg atgggcggcc agctcagcgg cagccaatgc ggattattac ttggcggctg
     2281 tgctaacgct gggcgcaatt gttcgatatg ctcccgttcg gccacgccgc agaacaccgg
     2341 agcaaggtgc atggcatcct gctctgagga ccagatacct cttacagaat cataatcgcg
     2401 gaaccagcca ttttgccaca tcagccgcgt tttgaccgta aactcgttgg ccacctcctg
     2461 ccagaaagca atgtcggcag tgtagggatt gggaagcgtt tcatcggcgt gcgttgatga
     2521 aagtatttcg gcccaggaag ccaggattgc cgcgccctga gcgatgctgg cctgaagatc
     2581 aaccgggcgc aggtgttgga tgatggtgcc acccgtctgc tgcgggccaa agcgaataga
     2641 tacatcctgg ccgctctccc atgagcaccc gtatatttcc catccttcac tgtcgcgacg
     2701 atgatcgagc caccagcgta ggtacgcggc cgcgccagga taaagccgct tcaaccagga
     2761 aagatcgccc gacctcagcc atagttgctg gcagcaccac agtgggtatc cccattccgg
     2821 tgcggtgccg cagatttgcc cgtcatcggc caccatatta aagctgccat cttcacgcat
     2881 gcagggcagg ttcggatgag gggcagactc gaaatgtccc agtataacct cagccgccag
     2941 ttcggggtcg gcatagctga gaaacagggt atccatagcc gcctccgcga gaacggtacg
     3001 tggtgcctgt atctgcatgc catcccagac gtgcgggatg gcgcttgcgg gaggacgcgt
     3061 caccatacgg agcgtctcaa aatcatagac gaagccccgt tgccagtgcg caggccagtc
     3121 gccggtcagt ttgggggcct gttgccagaa ggactcgtcg ttctcacggt gcatactctc
     3181 ggcctcgttg atcgaatcca tgccctctaa ccaatagaag agcgctttat cttgcgaaac
     3241 accgcgtgcc agtgcagcat gcagggtaat ggacgacggg gacgatccgc caagtatgag
     3301 tctgtatggc atcgccagcg cgcgatgctg ccaaccggta tcgggctgcc gctgggtgat
     3361 cctaggccag gggatgcgca cacccctcgc ccaggccgat agctcgtcaa gtgaggtggc
     3421 aaagcccatc gaatccgagg taagcgccgg tccgttagcg atgtcctgat tgtcagaaag
     3481 gcgtgcgcca tgcacaaaga catcgccctc actggctagc ccgagcatac cgcatcccgt
     3541 ttgacggtcc tgcaaagcat acaagccgtg ttcccacagc cggctggtat gagggttgtg
     3601 gacatgccgg tggatgaggt agcaggtgat cgggacgggt gcgggaaatg tcgtggccag
     3661 cgtgagggtg cagccaagca catgctcctg caccagaaaa tagcgtatgg tgagggtgag
     3721 ttcatactgg gaaacaatgc aggtatattc aaaccgattc ttcgtatgga gctggctgtt
     3781 gattgtacac cggtggcgca ggaaatcttc ctgagtgaca agcaccaggt cattgatgtt
     3841 gagcccgatg gtgagatgcg cgctatattg gaactgatta ttgacataac t
//
LOCUS       DASZAK010000682         5526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_51982, whole genome shotgun sequence.
ACCESSION   DASZAK010000682 DASZAK010000000
VERSION     DASZAK010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51982"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VGT44_12025"
     CDS             <1..401
                     /locus_tag="VGT44_12025"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEV2581570.1"
                     /translation="AWAALFSSGLLPDYMVTLEVTGRKSGRTISLPVAVAVVDGQRYL
                     VSMLGEQVNWVQNVRAAGGRAVLHSGGREEVHLEAVPAEQRAPILKAYLQRAPGARPH
                     VPVNKDAPLAEFEKVASAFPVFRVASHKRV"
     gene            867..1034
                     /locus_tag="VGT44_12030"
     CDS             867..1034
                     /locus_tag="VGT44_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581571.1"
                     /translation="MAEAMLVAALILAVVSVLSFAILPVKVRHAEGDATTLRMTGDRP
                     EQDVKSDAVTP"
     gene            1123..2337
                     /locus_tag="VGT44_12035"
     CDS             1123..2337
                     /locus_tag="VGT44_12035"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2581572.1"
                     /translation="MKARSIIISLTVSCALMMMGFGIATPIFARRLGELGSGVEVLSL
                     MAMASALAQFLLAPLMGALADRFGRRPIELLALLGLTITNLAFLVARSAEDYIVLRFL
                     QGAFSVGMLPAAMGMIADLAPEDHRTRWVAVIMSGYAVGFTFGPVIGGFLFERWGFIA
                     SFGVSALLDLLALCVACVIVPETREAAIRHLQPLVVAGSGQYELAASIPRPRSYFVAL
                     LLLDFIATFGMAFIEPQMVFYLYNALSLTTAQYGFIMGGYGMAMLVGQVALGQPGDRF
                     GRKPVIALGFLLSSALSLGLIIFDQFGSLVLAALLAGVGNALIAPRLGASYLDIAVPQ
                     HRSVVQGIRESAVSLGAVAGPLLAACISRWLAPQGIFTIAAVTTLAAVILALVMLKPQ
                     DHSVRPMAVTAP"
     gene            2459..3151
                     /locus_tag="VGT44_12040"
     CDS             2459..3151
                     /locus_tag="VGT44_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2581573.1"
                     /translation="MNELILVVDDEPKIVKLARDYLEQSGFRVVTAADGMSALAVAHR
                     ERPDLIVLDLKLPGKDGLDVCRTLRRESDVPIIMLTALAEETDRLIGLELGADDYITK
                     PFSPRELVARVRAVLRRVQGSVRQPGLILVADLEIDLNGRQVTRQGEAVTLTRSEFDL
                     LVMLAQHPGQAFTREQLLTRLYDVAYEGFARSIDAHIKNLRQKLEIDPGQLRYILTVF
                     GVGYKFSNEAPS"
     gene            3148..4299
                     /locus_tag="VGT44_12045"
     CDS             3148..4299
                     /locus_tag="VGT44_12045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2581574.1"
                     /translation="MKPGVSLPPHRRRFGHKPYDRHLRRRFLFLRFARIFGFLALLIL
                     GGLATLAWLVTRWLTGDGQMPVLVWVGGLLLGVGLPLVGWGIAGHAFQDIARPLADLM
                     DAADAVAVGDLRVHVAEESPGDFGRLAQTFNRMTRELERAEVQRRNFTADVAHELRTP
                     LHIIQGNLEGLLDGVYQPTAEHITALLDETRWLARLIDDLRLLSLAESGQLVLHKEAI
                     AVDELLTDISTSFCGQAESKGIQLYVAGKDKLAGMTVNADVGRLYQILGNLVVNALRY
                     TPSGGTITLKGERVPGGVHLIVRDTGQGIALEDIPSIFDRFWRGDTARSRMEGAGGGL
                     GLSIARQLALAHGGRIEVESQPGQGATFTVHLPADDGEASIGVEHLLLP"
     gene            complement(4232..5014)
                     /locus_tag="VGT44_12050"
     CDS             complement(4232..5014)
                     /locus_tag="VGT44_12050"
                     /inference="COORDINATES: protein motif:HMM:NF005868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2581575.1"
                     /translation="MQHQSQRVALITGGSRGIGAATALALADRGYDVALTYRNKATRA
                     REVVAAVTQYNVRGLALACDMTHSGEIACLFQRLVQWTDHLDVVVLNASGGLERALVG
                     ADPLYPMHINRDAQLQFTCAALPLMLQGSVVVFVTSHWAHLYGQMEQLPAYEPVAESK
                     HAGEQALRARQDEFAARGVRLIVVTGSLIEGTIAPKLLQRTAPGLIDQQRSITGTLLT
                     AEEMGKEIALAAMKTDLPNGSTVVAGAPLQSMLLHHLPGDGP"
     gene            complement(5045..>5526)
                     /locus_tag="VGT44_12055"
     CDS             complement(5045..>5526)
                     /locus_tag="VGT44_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581576.1"
                     /translation="DFRRLAREAMQRRITTLWSRLPQPLSPISAGEVQARLAEAYALA
                     LDRPVVPGSLTEAEQAKAAEMEEQLTSEAWLQLHAEDQDEPMRILKISLDAFVHAEEA
                     RVNGTAVGGTFFTRRDMIEQAILTGDLPEKKNHLLENRLVGVPLKQWREVVFEYAFQ"
BASE COUNT         1009 a         1600 c         1695 g         1222 t
ORIGIN      
        1 gagcgtgggc tgctctcttc tcctcgggtc ttctgcccga ctatatggtg actctggaag
       61 tgaccggacg caagtctggg cggacgatct cgctgccggt ggcggtggct gtcgtcgatg
      121 gacagcggta cctggtgtcg atgctcggag agcaggtgaa ttgggtgcag aacgtccgtg
      181 ctgccggggg aagagcggtg ctccacagcg gcggtcgtga ggaggtccac ctcgaagcgg
      241 ttcccgccga gcagcgcgcc cccatcttaa aagcatacct gcagcgagca ccgggagcgc
      301 gcccccatgt acccgtgaac aaggatgcac cacttgcgga attcgaaaag gttgcctcgg
      361 cctttccggt cttccgtgtg gcttctcaca agagggtgta gccagggcag ccatactcca
      421 gtccatcttt gcagcttcgc caatcaacac gccctttgtg gctctcgtct tcgtgattgg
      481 cgcgatcagt ttcatcttcc tcgcgttctt tgtgcgaggt gcagcgagag gtcagttgcc
      541 agcatcgctc ggcacgaagg agagctgagt gaaggcagag agcgcaagga tgctcgtgat
      601 tgaagcaagc atgcctgcaa aggagtttct tcaattcaca acatctcccc aacttcctca
      661 ctagttcctc acacctttgc catatgcttc tcttgaagag ggatccaggg tagctctgga
      721 cgctgctctg atgagtccaa tgcagtgact tcaccagaat cgcagatgcc ctcttcagaa
      781 agagaagcat atgaagcacc tcaccgcagg acccgtgcat tgctcagacc atagcggatg
      841 ccgccaatca tgccttcgtc tccggtatgg cggaggctat gctggttgcc gcgttgatcc
      901 tggctgtggt gtcggtgctg tcgttcgcca tcctcccggt caaggtgagg catgccgagg
      961 gcgacgcaac aacactgcgg atgaccggcg accgcccgga gcaagatgtg aagagcgacg
     1021 ccgtgacacc ctgacgtttc gggcgtacgc cacgtctgca ccaaacgtgg cgtacctcat
     1081 gcatcgcctc caggagaggg ggaagggaaa ggagagaagg gtatgaaggc acgttcgatc
     1141 atcatatctc tcaccgtaag ctgcgctttg atgatgatgg gctttggcat tgctacgccc
     1201 attttcgcca ggcgcctggg cgaactcggc tccggagtgg aggtcctgag cttgatggcc
     1261 atggcctcgg ccctcgcgca attcctgctg gcaccgctta tgggagcgct cgccgaccgc
     1321 ttcgggcggc gtcctatcga gctccttgcc ttgctgggtc tcaccatcac aaatctcgct
     1381 ttcctggtag cccggtctgc tgaggactat attgtgctac gcttcttgca gggtgcgttc
     1441 agtgttggaa tgctcccggc ggccatgggc atgatcgctg acctggcgcc agaagaccat
     1501 cgaacccgct gggtagcagt aatcatgagt ggctatgctg tggggttcac ttttggcccg
     1561 gtcatcggtg gtttcctctt tgagcgatgg ggctttatcg cgtccttcgg cgtctctgcc
     1621 ctgctggatc tcctcgctct atgcgttgcc tgtgtgattg ttcccgagac gcgggaagct
     1681 gccatccgtc atctacagcc gctcgttgtt gctgggtccg ggcaatatga acttgccgca
     1741 tcaattccac gtccccgctc ctactttgtt gccttgctcc tgctcgactt catcgccacc
     1801 tttggcatgg cgttcatcga accgcagatg gtcttctacc tctacaacgc gctttccctg
     1861 accacggcac aatatggttt tatcatgggt gggtacggga tggccatgct ggttgggcag
     1921 gtggcgctcg gccagccagg tgaccgcttt ggcaggaagc cggtaattgc gctcggtttt
     1981 ctgctcagtt ccgcgctctc tcttggcctg atcatctttg accagttcgg ctcgctcgtg
     2041 ctggcggcgc tgctagccgg ggtggggaac gcgctcatcg ctcccaggct gggcgcctcc
     2101 tatctagaca tcgcggtacc acagcaccgg tcggtcgtcc agggcatacg cgaatcggct
     2161 gtctcgctcg gcgcggttgc cggtccgctc ctggctgcgt gcatcagccg ctggctggct
     2221 ccacagggca tctttaccat tgccgctgtg accacgctgg cggcagtcat cctggcgctc
     2281 gtcatgctga agccacaaga ccacagtgtc cggccaatgg ctgtgactgc tccgtgagaa
     2341 aaacagacca gcttgtgagc atctcgtgag agaggagcag tacgcttctt caaaggaggt
     2401 tctgcccggt cgtgaagacg gtggtttgga tgtttcaatt agcgaagagg aaaggccgat
     2461 gaacgaactc atacttgtcg tggacgatga acccaaaatc gtcaaactgg cgcgagacta
     2521 cctggagcag tctgggttcc gtgtagtgac agccgctgat ggaatgtcag ccctggccgt
     2581 cgcacaccgt gagcgacccg atctgatcgt gcttgacctg aagctgccag gcaaggatgg
     2641 gctagatgtg tgccggacac tgcggcggga gtcagatgtg cccattatca tgctcaccgc
     2701 cctcgctgag gaaacagatc gcctgatcgg gttggagctg ggagcggacg actatatcac
     2761 gaaacccttc tccccccgcg agttggtggc gcgggtgcgt gcggtcctgc gtcgtgtcca
     2821 gggcagtgtc cgtcaaccag gcctgatcct ggttgctgat cttgagattg atctgaatgg
     2881 gcgccaggtg acacgccagg gcgaggcggt cacactgacg cgcagcgaat tcgatctgct
     2941 ggtcatgttg gcgcagcacc caggacaggc cttcacccgc gagcagttgc tcacgcgact
     3001 ctacgacgtg gcctatgagg gctttgcccg cagcatcgat gcgcatatca agaacctgcg
     3061 ccagaagctg gagatcgacc ctggccagct gcgctatatc ctcacggtct ttggcgtcgg
     3121 ctacaagttc agcaacgagg cgccatcgtg aagcctggag tgtccttgcc gccacatcgc
     3181 cggaggttcg ggcacaagcc ctatgatcga catctcaggc ggcggtttct cttcttgcgc
     3241 tttgccagga ttttcggctt cctggcactg ctgatcctgg gaggactggc aacgctggcc
     3301 tggctcgtga cgcgatggct caccggcgat ggtcagatgc cggttctcgt ctgggtcggc
     3361 ggcttgcttc tgggagtggg gcttccactg gtggggtggg gaatagcggg ccatgcgttt
     3421 caggacatcg ccaggccgct ggccgacttg atggacgcgg cagatgcagt ggcggtgggc
     3481 gacctgcgcg tccatgtcgc cgaagaaagt cccggcgatt tcggcaggtt ggcgcagacg
     3541 tttaatcgca tgacgcggga actggaacgc gccgaggtgc agcgccgcaa tttcaccgct
     3601 gacgtggcgc acgagctgcg cacgccgctg catatcatac agggcaacct ggaagggctg
     3661 ctcgacgggg tctaccaacc aacggcagag catatcactg ccctgctcga cgaaacgcgc
     3721 tggctggcgc gtctcattga cgacctgaga ctcctctccc tggcagagtc tgggcaatta
     3781 gtgttgcaca aagaggccat cgcggtagac gaattgctca ccgatatctc gaccagcttc
     3841 tgcgggcagg ccgagtccaa aggcatccag ttgtacgtcg ccggaaaaga taagctcgca
     3901 gggatgaccg tgaatgctga cgtaggacgg ctctaccaga tcctcggaaa cctcgtcgtg
     3961 aatgcactgc gttacacgcc ttccggcggg actattaccc tcaagggaga acgagttccg
     4021 ggcggcgttc acctcatcgt gcgtgatacc ggccagggca ttgcacttga ggatatcccc
     4081 tccatctttg accgtttctg gcgtggtgat acggctcgct cccgcatgga gggtgctggt
     4141 ggaggattgg ggttgtctat tgcgcgccaa ctcgcgctgg cgcatggtgg acgcattgag
     4201 gtggagagcc agccaggtca aggagcaacg tttacggtcc atctcccggc agatgatgga
     4261 gaagcatcga ttggagtgga gcacctgcta ctaccgtaga accatttggt aggtcggtct
     4321 tcatggctgc cagcgcaatt tcctttccca tctcctctgc cgtcaggagc gttccggtga
     4381 tgctgcgctg ctgatcgatc agaccaggag ctgtccgttg gaggagtttg ggggcgatcg
     4441 ttccttcaat cagacttcct gtcacgacaa tgagtcggac gcctctggct gcgaactcgt
     4501 cctggcgggc gcgtagagcc tgctcaccgg catgtttact ctcggcaacc ggttcgtagg
     4561 cgggcaattg ctccatttgc ccgtagagat gcgcccaatg gctggtgaca aagacaacca
     4621 cgcttccctg gagcatgagt ggaagggctg cgcaggtaaa ttgcagttgt gcgtccctgt
     4681 tgatgtgcat ggggtacagc ggatcagccc cgaccagcgc tcgctccaat cctcccgagg
     4741 cattgagcac gacgacatcc agatgatctg tccattgcac caggcgctgg aaaaggcacg
     4801 caatctcacc ggaatgggtc atatcacagg cgagcgcaag accacgcaca ttgtactgcg
     4861 tcacggctgc gaccacttcc ctggctcgtg tcgctttatt gcgataggtg agcgcgacat
     4921 catacccgcg atcggcgagc gcgagcgctg tagcggctcc aatgcctcgc gagccgccgg
     4981 tgattagagc aaccctctgg ctctgatgct gcatacctct cctgttctct ccctgtgaaa
     5041 gagatcactg gaaagcatac tcgaaaacga cttcgcgcca ctgtttcagg gggacgccga
     5101 ccagcctgtt ttctaaaaga tggttttttt tctcagggag gtcgcctgtc aagatcgctt
     5161 gctcgatcat gtcgcggcgg gtgaagaagg tgccgccgac cgctgttccg ttcacgcggg
     5221 cttcttcggc atggacaaag gcatcaagcg aaattttcaa tatccgcatc ggttcgtcct
     5281 gatcctctgc atggagttga agccatgctt cggacgtaag ctgttcttcc atttcggctg
     5341 cttttgcctg ttccgcctcc gtcagtgatc cgggtacgac cgggcgatcc agcgccaggg
     5401 cgtatgcttc ggcaaggcgc gcctggactt caccggcgga tatgggggat agcggctgcg
     5461 ggagcctgct ccacaaggtg gtgatgcggc gctgcatggc ctcgcgggcg aggcggcgga
     5521 agtctt
//
LOCUS       DASZAK010000683         2909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_444586, whole genome shotgun sequence.
ACCESSION   DASZAK010000683 DASZAK010000000
VERSION     DASZAK010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444586"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VGT44_12060"
     CDS             complement(<1..670)
                     /locus_tag="VGT44_12060"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2581577.1"
                     /translation="MTQDFYTSGEYLQENPTWHADESPWKVAGVLGILKKRRLQPKTI
                     GEIGCGAGEVLRLLQQHLAADCELWGYDISPKAIELCSSKANERLHFVLADRAQAADR
                     RFDLILILDVLEHLEDYFSLLREIHPTSDYKIFQLPMDISLRSVFLKRLTSFHKTYGH
                     LHYFTKETALQMVRDAGYEVVDFCYNGPTGMVPLPWEQVKSRPLLFPRLLLRLVKRIV
                     WKLSF"
     gene            868..1620
                     /locus_tag="VGT44_12065"
     CDS             868..1620
                     /locus_tag="VGT44_12065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV2581578.1"
                     /translation="MEETNVQQDAAAGQSTETSPSPGRDEANEHHYDASKYDRPSVTV
                     DVVMMSLRQRDLQALLVKRRSWPYEGMWAIPGGFVNIDESLEDAAKRELKEETSVEDV
                     YLEQLYTFGDPGRDPRTRVITVVYFALLDSDRLQVKAADDAVDVGWFSVYQLPPLAFD
                     HAEILQYTLNRLRGKLDYTTIAFNLLPEQFTLRELQRVYEIILHRKVDKRNFRKKILS
                     TGILEDTGAKKMEGTHRPARLYRFNPAAEAEL"
     gene            complement(1633..2772)
                     /locus_tag="VGT44_12070"
     CDS             complement(1633..2772)
                     /locus_tag="VGT44_12070"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV2581579.1"
                     /translation="METLRIGLDLTAAWRRPTGIFRYATEIGRNLLLLEETQPSIRYV
                     LFFAKEIHPDFVPLKDRFDAVICPTTNELLIKQFWFPATVPGLHLDVMHYPAFPPPYL
                     HPFGASTVMTLHDAGPWRFGKALTWHGRIYFRSLLTRGLHSCAHVITVSKHAKAEIGY
                     FLGERYFSKITVVPEAARAEFAIPCSDAFIQEVRARYQLPGDFFLTVATVEPRKNLVS
                     LLDAYKRLKQQLGEACPPLVIVGRKGWNCDDILGYMAELEEKVLFPGHVSDEELVALY
                     QSATSLVFPSLYEGFGLPVVEAMTAGCPVITSTASSLPEVAGDAGLLVDPLNVDEIAT
                     AMQRVLQEKELRASMISDGRAWAAHFSWEETARLTRDVYLRAAAH"
BASE COUNT          628 a          809 c          849 g          623 t
ORIGIN      
        1 cgaaggagag cttccacacg atgcgtttca ccagccgaag tagcagtctg gggaacagca
       61 agggtctgct tttaacctgc tcccagggca ggggaaccat gccggtcgga ccgttgtagc
      121 agaagtctac cacctcgtag cccgcgtcgc gtaccatctg gagcgcggtt tctttggtga
      181 agtagtgcag atgcccgtag gttttatgaa agctggtcag gcgcttcagg aagaccgagc
      241 gcagcgagat gtccatgggc aactggaaga ttttgtagtc gctcgtgggg tggatctcgc
      301 gtaagaggct gaaatagtct tcaagatgct caagcacgtc gaggatcagg atcaggtcga
      361 agcgtctatc cgccgcctgc gcccggtcgg ctaagacgaa gtggagtcgc tcattcgcct
      421 ttgaactgca taactcgata gcctttggag aaatgtcata cccccataac tcacagtctg
      481 ctgccaggtg ctgctggagc agtcgcagca cctcgccagc gccgcagccg atctcaccga
      541 tcgttttggg ctggagtcgc cgcttcttga ggatgcccag cacaccggcg actttccagg
      601 gagattcatc cgcgtgccag gttggatttt cctgaaggta ttccccgctc gtgtagaagt
      661 cttgcgtcat atgcttgctc tacgtttctc tttataacag tgcgtcatcc aatggtgttc
      721 ccgtgttctc gcctcgtgta ttgtaccttg cctgccacga cgggacaagt caccattacc
      781 cgcgctcttt acgccgcgtg ttattatgta gctgctagag attctctgca tcaaaacgac
      841 ttcattgaaa gcgatatcca acgaggaatg gaagaaacga acgtgcagca ggacgcggct
      901 gctggccagt caacggaaac gtcgccatcg ccaggccggg acgaggcgaa cgagcatcac
      961 tacgatgcaa gcaaatatga tcggccctcg gtaacggttg acgtggtgat gatgagcctg
     1021 cgccagcgcg acctgcaggc cctgctggtc aaacgacgct cctggccgta tgagggtatg
     1081 tgggctattc ctggcgggtt cgtcaatatc gatgaatcgt tggaagatgc cgccaagcgc
     1141 gaattgaagg aagagaccag cgtcgaggac gtctacctgg aacaactata caccttcggc
     1201 gatcctggtc gcgacccgcg cacccgcgtc atcaccgtgg tctattttgc cctgctcgac
     1261 tccgaccggt tacaggtcaa ggcggctgat gacgcggtcg atgtgggctg gttttcggtg
     1321 tatcagctgc cgccgctggc cttcgatcac gctgagattc tccagtatac gttgaaccgg
     1381 ctgcgcggca agctggacta caccactatt gccttcaacc tgctgccgga gcagttcacg
     1441 ctgcgcgaat tgcagcgcgt ctacgagatc atcctgcatc gcaaagtgga caagcgcaat
     1501 tttcgcaaga agattttatc gacgggtatc ctggaagata ccggcgcaaa gaagatggag
     1561 gggacgcacc gcccggcgcg cctctatcgc tttaacccgg cggctgaagc ggagttgtag
     1621 ttgtccctcg cgtcaatgtg ctgcggctcg caaatacaca tctcgcgtca gcctggcggt
     1681 ttcttcccag gaaaaatgcg cggcccacgc tcgcccatcg gaaatcatgc ttgccctcag
     1741 ttccttttcc tgtaggacgc gctgcatagc ggtcgcaatc tcatcgacat tgagcgggtc
     1801 taccagcaat cctgcatctc cggccacttc cggcaaggag gaggccgtcg aggtaatcac
     1861 gggacacccg gcggtcatgg cctcgaccac cggcaaaccg aatccttcat acagcgaggg
     1921 gaagacaagg ctggtagccg attggtagag cgccaccagt tcttcatccg aaacgtggcc
     1981 ggggaacagc actttttcct ctagctcggc catgtagccg aggatatcgt cgcagttcca
     2041 ccctttgcgc ccgacgatca cgagtggggg acacgcctcg ccaagctgct gtttgaggcg
     2101 tttgtaggca tcgagcaggg aaacgaggtt cttgcgcggc tctacggttg cgaccgtcaa
     2161 gaaaaagtct cctggcaact gatagcgcgc gcgaacctct tgtatgaagg cgtcgctgca
     2221 tgggatggcg aactctgccc tggcggcttc aggcacgacc gtgattttcg agaaatagcg
     2281 ctcacccaga aaatagccga tctcggcctt agcatgtttc gagacggtaa ttacgtgcgc
     2341 gcaggagtgg aggccgcgcg tcaacaggct gcggaagtag atgcgcccgt gccaggtcag
     2401 agcttttccg aagcgccagg gaccggcgtc atgcagcgtc atgacggtcg aggccccgaa
     2461 tgggtgtaga tagggaggcg gaaaggcggg ataatgcatg acatccaggt gaagccccgg
     2521 cacggtggcc gggaaccaga actgcttgat cagcagttca ttggtggtcg gacagatcac
     2581 cgcgtcaaag cgatctttca gtggaacaaa atcgggatgg atctctttgg caaaaaacag
     2641 cacatatcga atagatggct gcgtttcttc caggagcagc aggtttctgc cgatttctgt
     2701 ggcgtatcga aaaattccag tcggacgccg ccaggcggca gtcaggtcca ggccaatccg
     2761 taatgtttcc atgagtagcg aacactcctt gtcagagtct ctgctttata gcggcgcatg
     2821 tattatctac acgccagtgt tagctgatag gacctcccta ggtgtgtaga cagcacctcg
     2881 agccggttcg ccgcgaccct gcgaagcaa
//
LOCUS       DASZAK010000684         9152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_408929, whole genome shotgun sequence.
ACCESSION   DASZAK010000684 DASZAK010000000
VERSION     DASZAK010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9152
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408929"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2373)
                     /locus_tag="VGT44_12075"
     CDS             complement(<1..2373)
                     /locus_tag="VGT44_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEV2581580.1"
                     /translation="MLRKDRILETLRSMCTQRPAQSGVLRRYSGFSAEEVAEQALVDR
                     TNASRDLNLLVQEGAVERIPGRPVLFTIKTPSIPRDGNPQANNVSEDAHQKLYGHSSS
                     IARTDHGTATEQAPGKPQAIVATIQYGAVVTSFDTLIGSNGGLKVAIQQAKAAMLYPP
                     RGLHTLLCGPSGVGKTTLARLMHNYALELEALPLDAPFISFNCADYANNPQLLMAHLF
                     GVVRGAFTGADRDREGLVEQANRGILFLDEVHRLPPEGQEMLFYLMDRERFRRLGDVH
                     ERHSSLLLLAATTEDPAAALLPTFRRRIPMTITLPGLSERTIMERYELVRAFFTTECS
                     SIGSNIHVEPQVLHAFLVYDCPGNVGQMRTDVQLTCARAYLDYRTHNSSDLYVHLGVL
                     PDHVRQGLLRTAELHRYLEPVKHLLETTHIFTPTGLNLDNVPESAHDLYDTISADLNR
                     LRTSGLPDGEINRLLHLDIQDYFIRFADQVSAKQAGTPTNLIDERIAEVSESIVQFAE
                     EQLGRRLPEKLALVLAMHLTSTLEHLAQGRQIAGLAIQSVRRTYPIEYEVARAALAHF
                     RSTLGVSLPESEMDVLAVLLANAEALLSSELTTVGIVVAAHGRGIAAGLAELANTLVG
                     GNSVRWVELTLDETPEQLLSKVVHWVQVADQGSGVLLLVDFASLLSLGELVVSRTGIQ
                     VRAVANVSAPMLVEAVRRAQRTTHITLDQLAASLANVQSASQIKPTAPGSFLTSFGQE
                     QWIASGEHDAPAYRYASQQAQRVILSICLTGFGSASKIAELIEEHIPGLRN"
     gene            2745..4730
                     /locus_tag="VGT44_12080"
     CDS             2745..4730
                     /locus_tag="VGT44_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV2581581.1"
                     /translation="MNTITRSYQPIHSYGVIGDCHSVVLVAPDGSVDWGCLPDFDSPA
                     IFCRLLDAERGGYFQIAPTDASISGTQRYLRGSNVLQTRFEGANGEAILTDFMPVETL
                     SAWQFRELNNNTWTREDGACHCLVRIIECTSGELPITMTLKATPDYAAAPGEATLIPG
                     GLGAVISSGQQHIGLGVVGAYRLPSFTMQIVQNEGESHPAVELKATLQEGERLLFAVG
                     MGRNARAARRLAEEELHQRNFDWELAHTLHCWRTWLKSRTFEGAYDDWVQRSALVLKM
                     MTYAPTGAIIAAPTTSLPEEIGGVRNWDYRYTWLRDATFTLYALNVLGFTEEAHAFTH
                     WLRRLSYSRGEDLQIMYGIRGERELTERELPHLSGYCNSRPVRIGNEAANQKQLDVFG
                     EVLDCIHLFRRQGGFERYGEQLEGPLWSMMRLLVEHVCEHWQETDQGIWDVRGGPRHC
                     VYSKVMCWGALDRGIRAAEQLNLEADLQRWRMVRDQIRADILMRGYNTRIGAFTQSYD
                     SDALDASNLLLSLVRFIAPDDPRMRSTVDRIMQQLTDERGFVYRYLAEDGLAGGEGTF
                     TICTFWLVDNLALQGRVGEARSLFERLLSYAGHLGLFSEEIDQHGTMALGNYPQAFTH
                     IALINSAYNLKMAQMRVGEHHTDPIIAAIRLQGQGNK"
     gene            4739..5929
                     /locus_tag="VGT44_12085"
     CDS             4739..5929
                     /locus_tag="VGT44_12085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV2581582.1"
                     /translation="MQYPTASLKPHKEEILQSGHLWIFSGALQQPPSWIEPGGLVDVK
                     SATGQFVARGYYNPRTDIAIRVLTRDSDEAIDFAFLRRRIRSALELRHVFDPDSTNAY
                     RLINAEGDGLPGLVVDRYAEILVAQVHTLGMERLSEQVIEALAEESGARGILLRNDGQ
                     ARRREGLEVEEPVVAVGGVPTRVAIRENGVLFQVDPWEGQKTGFFLDQRDKRFALRKY
                     AQAKRVLNCFSYSGGFSVNAALANSATRVTSVDISAPAIEAAREHFLMNGLDPNLHEF
                     RSADVFDYLEEAHRGGELFDVVVLDPPAFAKSQATRTPALKAYRRLNTLGMQVLRPGG
                     ILLTCSCSGVIGMDDLLGAVAHSAQRLQRTVQVLETYTHGVDHPVNLAMPETDYLKAV
                     FCRL"
     gene            complement(6111..6902)
                     /locus_tag="VGT44_12090"
     CDS             complement(6111..6902)
                     /locus_tag="VGT44_12090"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2581583.1"
                     /translation="MSILYEKRGRVAYITINRPEARNAIDPETSQELSQAWKDFRDDD
                     DLWIGVLTGAGDKAFSAGADLKTFIPRLTGALGDSQARDATADDSFGGITRGFECWKP
                     MIAAVNGYCFAGGFEMMLACDLRIAAENATFGLTEVRWGIIPGGGGTQRLPRAIPLAK
                     AMEIILMAEAISAEDAYQMGLINKVVPQGELMQEVERWVAILLERGPLALRAAKQAIL
                     QGLSMPISEGLQLEQRLFGSLLRTEDAREGPTAFAQKRKPDFKAR"
     gene            7119..8477
                     /locus_tag="VGT44_12095"
     CDS             7119..8477
                     /locus_tag="VGT44_12095"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2581584.1"
                     /translation="MAQTIDESRAQLIFSLQQQAEELTQLNRIAIALTSEFDLQRLLQ
                     MITDAARRVTSAEYAAFFLIPELAGEDSRQSPNKVIFNLAAISGATPAIEKHFLRLGP
                     VEGIGLLEPVFWEGDSVVVDDVHSDPRYVGVPRGHIPVRSFLGVQLRTREGANLGAFL
                     IGDTHPSRFNTRHVELIEALSAQAAVALHNAQLIARERRAMEEHADRLEREVRERTAE
                     LEQRNLELSQYATDLQTLHHELTEAQKRQMLVDERSRIAQELHDRVQQTLFTIGLKAN
                     WIMEQLPPASTQVHALRTIRQLASLGTAQVRDAIFALSSDELQGGSLVGMLYTLIQNL
                     RESSSIEVDLVVDEWAVPLPAHLENMLYTIAQEALSNARRHAQATTAIVTVQVSNDQA
                     MLVVQDNGIGLPAHMFQNYRNNSMHLGLRGMHHHVEELKGQFSLTNGEEGGLIVKAVI
                     PL"
     gene            8474..9112
                     /locus_tag="VGT44_12100"
     CDS             8474..9112
                     /locus_tag="VGT44_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2581585.1"
                     /translation="MIRLLIVDDHEMVREGLKAMLVTEPDFMIVGEASNAEQALVCVE
                     RAQPDVILLDIRLPGVSGIEVCRAVTERYPDISVIFLTTFTDEPLIAQCIQAGARGFI
                     IKDIERFDLKRSIRAVARGEAAIDSKAAVAVLAQLRKSPALGSEPGPEPLSSQQIVIL
                     RLVAQGLSSREIATRLYLSENTVKGYVQEILHRLSVKNRTEAVMVAVKQGWL"
BASE COUNT         1860 a         2604 c         2743 g         1945 t
ORIGIN      
        1 gttacgcagt cctggtatat gctcttcgat cagttccgca atcttgcttg cgctgccaaa
       61 accggtcagg cagatggaga gaatgacacg ctgagcttgc tgtgaggcat aacggtaggc
      121 tggcgcatca tgttcgccag aagcaatcca ttgttcctgg ccaaaagatg taagaaagct
      181 tccaggcgca gttggcttga tctgcgaggc agattggaca ttggcgaggc ttgcggctaa
      241 ttggtcgagg gtgatatgcg tcgtccgctg cgcgcgcctg actgcttcaa ccagcatcgg
      301 tgccgagacg ttcgcgacag cgcgcacctg aatgcccgtg cgactgacaa ccagttcgcc
      361 gagcgagagc agcgaggcaa aatcgaccag cagcagcacc ccgctgccct ggtctgccac
      421 ttgcacccag tggaccacct tgctgaggag ctgctccggc gtttcgtcaa gcgttagctc
      481 gacccagcgc accgagttcc cgccgaccag ggtattcgcc agttccgcca gtcctgctgc
      541 gatgccccgg ccatgcgcgg caacgacaat accaaccgtg gtcagctcgc tgctcaacag
      601 cgcctcggcg ttggccagca gtaccgccag cacgtccatt tcgctctctg gcagggacac
      661 acccagcgtc gaacggaaat gtgccagggc tgcgcgcgcc acctcatatt cgatggggta
      721 cgtgcgccgc accgactgaa tcgccaatcc cgcgatctgt cgtccctgcg cgaggtgttc
      781 gagcgtactg gtcagatgca tggccagcac cagagccaat ttctccggca gccgccgacc
      841 gagttgctcc tcggcgaact gcacgatgct ctcgcttacc tctgcgatac gctcatcgat
      901 caggtttgtc ggcgtccccg cctgcttcgc gctgacttga tcggcgaagc gtatgaagta
      961 atcctgtata tccaggtgga gcaggcgatt gatttcgccg tcgggcaacc cgcttgtgcg
     1021 cagccggttc agatcggcgc tgatcgtatc gtagagatcg tgcgccgatt ctggaacgtt
     1081 gtcgaggttg agacctgtcg gtgtgaagat gtgtgtggtc tcgagcaggt gcttgacggg
     1141 ttccaggtag cggtgcagct ctgccgtgcg cagcagtccc tgacgcacgt ggtctggcag
     1201 cacgcccagg tggacataca gatcgctcga gttatgtgtg cgatagtcca ggtacgcgcg
     1261 ggcgcaggtc aattgcacat ccgtgcgcat ctgcccaaca ttgccgggac aatcgtagac
     1321 caggaaggcg tgcagcactt gcggttcgac atggatgtta gagccaatgc tggaacattc
     1381 cgtcgtaaaa aaggcgcgca ccagctcgta gcgctccatg atggtgcgct cgctcagacc
     1441 cggcagggta atcgtcattg gaatgcggcg gcggaaggtc ggcagcagcg ctgctgcggg
     1501 gtcttccgta gtggccgcca gcagcaagag ggaagagtgc cgctcatgca catcgcccag
     1561 gcggcggaat cgctcgcggt ccatcaggta aaaaagcatc tcctggccct cgggcgggag
     1621 gcgatgcacc tcgtccagga aaaggatgcc gcgattggcc tgttcgacca gcccctcgcg
     1681 gtcacggtca gcgccggtaa atgcgccgcg cacgacgcca aacaggtgcg ccatgaggag
     1741 ctgtgggtta ttggcgtaat cggcgcagtt gaagctgatg aacggcgcgt cgagcggtaa
     1801 cgcctccaat tcaagggcat agttgtgcat cagccgtgcc agggttgttt tcccgacgcc
     1861 gcttggaccg cagagcagcg tgtgcaagcc gcgcggcgga tagagcatgg ccgctttggc
     1921 ctgttgaata gcgaccttga ggccgccgtt gctgccgatc aacgtatcaa agctggtcac
     1981 aaccgcccca tactgaatag tagctacgat ggcctgtggc ttgccgggcg cctgttctgt
     2041 tgctgttcca tgatctgttc gagcgatgct tgagctatgt ccatacagtt tctgatgcgc
     2101 atcctcggat acgttgttag cttgtggatt gccgtcgcgg ggaattgaag gcgtcttgat
     2161 agtaaacagg acggggcgac cggggatgcg ctcaactgca ccttcctgaa ccagtaaatt
     2221 gagatctctg ctggcgttgg ttcgatctac cagcgcctgc tctgctactt cttcggcgct
     2281 gaagccactg taacgacgta aaacaccgct ttgtgctggt cgttgtgtac acattgatcg
     2341 tagagtttcc agaatgcgat ctttgcgcag cacgcaacga ctcctttcag gtcaaccatc
     2401 atgggtggat acatgaaaat gtaacattcg acgtaatacg gggcaaaagg actcgacgtt
     2461 gaatcaaatt gtccagtttt tcactgaaag tgtgtctgta tatgtaatac gtcatgtgtg
     2521 ccttgcgaat tcacagaaag gggcaaattg tccagtttat gagtttatct catcttgctc
     2581 catacgggca tacaaatgta ttaggctctt gttatcttgg aggaaaaata tgttacctat
     2641 gtaatgcctt ttccgttcag attcgatata ggaaccttag aaatcgctta gaaaatcttc
     2701 acacgatttt cactgtaaat tttcactaaa aggacggggt agggatgaat accatcacac
     2761 ggtcttatca acctattcat tcgtatggcg tcatcggaga ctgccattct gttgtactgg
     2821 tagcgcccga cgggtcggtt gactggggct gcctgccaga cttcgacagc ccggcaatct
     2881 tttgccgcct actggacgcc gagcgcgggg gatacttcca gatcgcgccg accgatgcat
     2941 ctatcagtgg gacgcagcgc tacctgcgcg gcagcaacgt gctgcaaacg cgctttgaag
     3001 gtgcgaatgg ggaagcgatt ctaaccgact tcatgccggt agagacgttg agcgcttggc
     3061 aattccgcga actgaacaac aatacctgga cgcgcgagga tggcgcatgc cattgcctgg
     3121 tgcgcatcat cgagtgtact tctggcgagc ttcccattac gatgaccttg aaggccacgc
     3181 ctgattacgc tgcagctcct ggagaggcga cgctgatacc gggtggccta ggcgcggtga
     3241 tctccagcgg gcagcagcat atcgggctgg gcgtggtcgg cgcgtatcgg ctgccatcct
     3301 tcaccatgca aatcgtgcag aacgagggcg agtcgcatcc ggctgtcgaa ctcaaggcga
     3361 cgctgcaaga gggcgagcga ctgctcttcg cggtaggcat gggccgcaat gccagggccg
     3421 ctcgccgctt agccgaagag gaactgcacc agcgcaactt tgattgggag ctggcgcata
     3481 cgctgcactg ctggcgcacc tggctgaaaa gccgcacctt tgaaggagcc tacgacgact
     3541 gggtgcagcg cagtgcgctg gtgctcaaaa tgatgaccta cgcgccgact ggggcgatta
     3601 tcgcagcgcc taccacatcg ctgccggagg aaatcggcgg ggtgcgcaac tgggactacc
     3661 gttacacctg gctgcgcgat gctaccttca cgctctacgc gctgaatgtc ctcggcttca
     3721 cggaagaggc acacgccttc acacactggc tgcgccgcct ctcctattcc aggggcgaag
     3781 acctgcagat catgtacggc attcgtggcg agcgcgaatt gaccgaacgc gaactcccgc
     3841 acttgagcgg ctattgcaac tcgcgcccgg tgcgcatcgg caatgaggcc gcgaaccaga
     3901 aacaactgga tgtcttcggc gaagtgctgg attgcatcca tctcttccgg cgacagggcg
     3961 gctttgagcg ttacggcgag caactcgaag ggccgctctg gtcgatgatg cgcctgttgg
     4021 tggagcatgt ctgcgagcac tggcaagaaa ccgaccaggg gatctgggac gtgcgcggcg
     4081 gcccacgcca ctgtgtgtat tccaaagtaa tgtgctgggg tgcgctcgat cgcggcattc
     4141 gcgcggcgga gcagttaaac ctggaagccg acctccaacg ctggcgtatg gtgcgcgatc
     4201 agattcgcgc ggatatcctg atgcgcgggt ataatacgcg cattggcgct tttacgcagt
     4261 cctacgatag cgacgcgctc gatgcctcga acctcttgct gtcgctcgtg cgtttcattg
     4321 cgcccgacga tccgcgtatg cgctcgacgg tggatcgcat catgcagcag cttaccgatg
     4381 aacgcggctt tgtgtaccgc tatcttgccg aggatggtct agccggtggc gagggaacat
     4441 tcaccatttg cactttctgg ctggtagata acctggcgct gcaggggcgt gttggtgagg
     4501 cgcgctcgct tttcgagcgg ctgttgagtt atgccggcca cctgggcctc ttctccgaag
     4561 aaatcgacca gcatggtaca atggcactgg gcaactaccc gcaggcattc acccatatcg
     4621 ctctgattaa cagcgcctat aacctgaaaa tggcgcagat gcgcgtgggc gaacaccata
     4681 ccgacccgat tatcgcggcg attcgcctgc aagggcaggg caacaagtaa actagcctat
     4741 gcagtatccg acggcaagcc tcaagccgca taaagaggaa atcctacaaa gcgggcatct
     4801 gtggattttt tcaggcgcgt tgcagcagcc gccttcctgg atcgagccag gcgggctggt
     4861 cgatgttaaa tcggcaacgg gccagtttgt ggcgcgcggg tactacaatc cgcggaccga
     4921 tatcgctatt cgcgtgctga cgcgcgatag cgatgaggcc atcgactttg ctttcttgcg
     4981 ccgccgcata cgcagcgcgc ttgaactgcg ccacgtgttt gaccctgaca gcacaaacgc
     5041 ctaccggctc atcaatgccg agggcgatgg gttgcccggc ctggtggtgg atcgctacgc
     5101 cgaaattctg gttgcgcagg tccatacgct gggaatggaa cggctgagcg aacaggtcat
     5161 cgaagcgctg gcggaggaat cgggcgcgcg cggtatcctg ctgcgcaacg atggccaggc
     5221 gcgccgccgc gaggggttgg aggtcgagga gccggtcgtg gccgtcggcg gcgtcccaac
     5281 acgggtagcc atccgagaaa atggcgtcct tttccaggtc gatccctggg agggtcaaaa
     5341 aaccggcttc tttctcgacc agcgcgacaa acgctttgcg ctgcgcaaat atgctcaggc
     5401 aaagcgcgtt ctcaactgct tcagctatag cggcggtttc tccgtcaacg cggcgctggc
     5461 aaacagtgca acccgcgtaa ccagtgtaga catctccgcg cccgccatag aagcggctcg
     5521 cgagcatttt ctgatgaatg gtctcgaccc gaacctgcac gaatttcgta gtgccgatgt
     5581 atttgattac ctggaagaag cgcatcgcgg gggtgagcta ttcgatgtcg tcgtactcga
     5641 tccgcccgcc tttgctaaat cccaggcaac ccgcacaccg gcgctcaaag cctatcgtcg
     5701 cctcaatacg ctggggatgc aggtcttgcg tccaggtggc attctgctga cctgctcgtg
     5761 ttcgggggta atcggcatgg acgacctgct cggcgcggtg gcccactcgg cgcaacgtct
     5821 ccagcgcacg gtgcaggtgc tggagacgta tacacatggc gtggatcacc cggttaacct
     5881 ggccatgccg gagaccgatt atttgaaggc ggtgttttgc cgtctataaa agtcatcgta
     5941 gggattcgat gatggatgtt cacaaacaaa atcggcataa tggtgtcatc ctgagcgcag
     6001 cgaaggatct cggtacccaa ctcatcagat ccttcgctgc gctcaggatg acattatcgg
     6061 tattggtcgt gaccattcat catcgcgtcc ctacgacgag cggggcatta tcaacgcgct
     6121 ttgaaatcgg gtttgcgttt ctgcgcaaag gcggttgggc cttcgcgcgc atcctctgtg
     6181 cgcagcaggc tgccgaacaa acgctgctca agttgcaatc cttcgctgat cggcatactc
     6241 agtccctgga gaatggcctg ttttgccgcg cgcaaggcca gggggccgcg ttcgagcagg
     6301 atggcgaccc aacgttcaac ctcctgcatc agctcgccct gcgggacgac tttattaatc
     6361 agacccatct ggtaagcatc ttcggcgctg atcgcttcgg ccatcaagat aatctccatc
     6421 gccttcgcaa gaggtatggc gcggggcaga cgttgtgtgc cgccgccgcc ggggatgatg
     6481 ccccagcgta cctctgtcaa gccaaaggtg gcgttttcgg cggcgatgcg caaatcacag
     6541 gctagcatca tctcgaagcc gcccgcgaag cagtagccgt tgacagcagc gatcatgggt
     6601 ttccagcact caaagccgcg tgtgataccg ccaaagctgt cgtctgcggt tgcatcgcgc
     6661 gcctggctgt ctcccagcgc gccggtcagc cgagggatga aggtcttcag gtcggccccg
     6721 gcggaaaatg ctttgtcgcc cgcgccagtt agtacgccaa tccacaggtc atcgtcatcg
     6781 cggaaatcct tccatgcctg gctgagttcc tgcgaggtct ccgggtcaat ggcattgcgc
     6841 gcctctggcc ggttgatggt gatgtaggcc acgcgcccgc gcttttcgta gagaatgctc
     6901 attcactgct cctcactatg ttagttggcc gacagacgtg taggggagca attcattgcg
     6961 ccctcgtgaa agcgcttagc cagggacgag ggcgcaatga attgctcccc tacagggccg
     7021 ccatgggtgc agtgtagcac attcccgtcc gtcatgtgtt atactggtta cattcccctt
     7081 ctcttctaac gaatggtttt tgatggccgg ggtaagcaat ggcgcaaaca atcgatgaga
     7141 gccgggcgca actgattttc agcttgcagc agcaggcgga agaactgacg cagctcaacc
     7201 gtatcgcgat cgcgttgacc tctgaatttg atctgcagcg cctgttgcag atgattaccg
     7261 atgccgcgcg cagggtaaca tcggcggaat acgccgcctt cttccttatt ccagagctgg
     7321 caggagagga tagccgccag tcgccgaaca aggtcatctt caacctggct gccatatcag
     7381 gcgcgacacc ggcgattgag aagcatttct tgcggttagg gccagtagaa ggcattggcc
     7441 tgttggagcc ggtcttctgg gagggcgatt cggtagtagt ggatgatgtg catagcgacc
     7501 cgcgctacgt cggtgtgccg cgcggccata ttcctgtgcg cagctttctc ggcgtgcagt
     7561 tgcgcacccg cgaaggcgca aatcttggcg cgtttctcat cggcgatacc cacccatcgc
     7621 gcttcaatac ccgccatgtc gagttgatcg aggcgctcag cgcgcaggca gctgtggcgc
     7681 tgcacaatgc gcaattgatc gcgcgcgagc ggcgcgccat ggaagaacac gcggatcggt
     7741 tggaacgcga agtgcgcgag cgcacggcag agctggagca gcgcaacctg gaattgagcc
     7801 agtacgctac cgatctccag acgctgcatc acgaattgac cgaggcgcag aagcgccaga
     7861 tgctagtcga tgaacgcagt cgtatcgcgc aagaattgca tgaccgcgtg cagcaaacgc
     7921 tctttacgat tgggctgaaa gccaactgga ttatggagca actgccgcct gcttcaacgc
     7981 aggtacatgc cctgcgcact atcaggcagc ttgcgtcgct tggcaccgca caagttcggg
     8041 atgccatctt cgcgctctct tccgatgaat tgcagggggg cagcctggtg ggaatgctct
     8101 ataccctcat ccagaatctg cgcgaatcgt cctcgattga ggtcgacctg gtagtcgatg
     8161 aatgggcagt cccgttgcct gcccatctcg agaacatgct ctataccatt gcgcaagaag
     8221 cgctctcgaa tgcgcgccgc catgcccagg cgacgacggc catagtgacg gttcaggtga
     8281 gcaacgacca ggcgatgctc gtcgtgcagg ataatggcat tggtttgccc gctcatatgt
     8341 ttcaaaacta tcgcaataat tcgatgcacc tgggcctgcg cggaatgcat catcatgtcg
     8401 aggaactcaa agggcagttt tctctgacaa acggcgagga aggcggcttg attgtgaagg
     8461 cggtgattcc gctatgattc gcctgttgat tgtcgatgac cacgaaatgg tgcgcgaggg
     8521 gctgaaggct atgctggtga cggagccgga tttcatgatc gtcggcgagg cgtccaatgc
     8581 cgagcaggcg cttgtctgtg tcgaacgcgc acagcccgat gtgatcctgc tcgatattcg
     8641 tctacctggc gtgagtggta tcgaagtctg tcgcgccgtc accgagcgct atccagatat
     8701 ctccgtgatc tttttgacca ccttcaccga tgagcctttg atcgcgcaat gtatccaggc
     8761 gggtgcgcgc ggcttcatca tcaaagatat cgaacgcttt gacctcaaac gctcaatacg
     8821 cgcggtggcg cgcggcgaag ctgccatcga ctcgaaagcg gcggtcgcgg tgctggccca
     8881 attgcgcaaa agtccggcgc tcggaagcga acctggacca gaaccgctca gctcgcagca
     8941 gatcgtcatc ctgcgcctgg tggcgcaggg cctgtcgagc cgcgaaatcg caacccgcct
     9001 ttatttgagc gagaataccg tcaagggcta tgtccaggaa atcttgcatc gcctgagcgt
     9061 caagaaccgc acggaagccg tgatggttgc ggtcaagcag ggctggttgt aaagcagggc
     9121 tttcggcgct ccttcttgtc gaaccctacg aa
//
LOCUS       DASZAK010000685         1798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_444637, whole genome shotgun sequence.
ACCESSION   DASZAK010000685 DASZAK010000000
VERSION     DASZAK010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444637"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VGT44_12105"
     CDS             complement(<1..542)
                     /locus_tag="VGT44_12105"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEV2581586.1"
                     /translation="MEVFDTPIPLTTSDMSSDALSPGELAFWVAINRVSGIGPATFKA
                     LLDYFSDDAQAAWQADRKTLAQAGLNSRVVDNLVKLRSTLSLAGEMEKLARQRVRLLT
                     WRDAAYPPLLREIADAPPILYTYGKLTDADRFALAVVGTRNSSTYGKQVTERMVTDLA
                     KGQVTIVSGLALGIDTIAHTA"
     gene            complement(572..>1798)
                     /locus_tag="VGT44_12110"
     CDS             complement(572..>1798)
                     /locus_tag="VGT44_12110"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HEV2581587.1"
                     /translation="DAAGTTYIQPQQQLTTIDTRFTWQHLNRGLLQVNWYDRPQDFGQ
                     VILNSASSSIDRISGNLGGGLQSSINLWVYATDQDFQGSLPPGSYEWVGGIAFPELSE
                     ASIVVASIYDTTLSRDMPHELTHLVFHQLIGLQTSVPTWFDEGMAVFNQQFHEQAMID
                     RFNRALDTHSLLRLDNISFGFPADSDQAYLAYAQSWNLVQYMYTTFGQPKMIQFIKDL
                     SSLSLDFNQAMLAALGIDPIHLENRWRLHLNQPGIALPPGEATPTPQITARPTSHPNG
                     TGSASGDDRSWALILLGGALVLVALVGLIVLLVSLAARRKSAGTSPATNARPYSNGIP
                     VPPFDPSIYARDSMYMRPSGASPQSMLSSASPPAQPVQPAPGEWSPFAAGQEYSGHRP
                     GPSRPVTPPYPQAPQE"
BASE COUNT          370 a          476 c          576 g          376 t
ORIGIN      
        1 gcggtatggg caattgtatc gatgccgagc gccaggccgc tgacaattgt gacctgcccc
       61 tttgccaggt ccgtcaccat gcgttccgtg acctgcttgc cgtaggtgga ggagttgcgc
      121 gtcccgacca cggccagcgc gaaacggtcg gcatcggtga gcttgccgta ggtatagagg
      181 atcggcgggg cgtcggcgat ttcgcgcagc agcggtggat aggctgcgtc ccgccaggtc
      241 aagaggcgca cacgctggcg cgccagcttt tccatctcgc ctgctagaga aagagttgag
      301 cgcagcttga cgagattatc cacgacgcgc gagtttaacc cggcttgcgc cagcgtcttg
      361 cggtcggcct gccacgcggc ttgcgcgtcg tctgaaaaat agtcgagcag cgctttgaag
      421 gtggccggac cgatgccgct cacgcggtta atagccaccc agaaggccag ttcaccgggc
      481 gagagggcgt cggaggacat gtcggacgtg gtaagcggta tgggagtatc aaacacttcc
      541 atcagatgtt tcctttacac tatgttgtgc atcattcctg gggagcctgt ggatatggcg
      601 gagtaactgg gcgtgatggc cctgggcgat ggcctgaata ctcctggccc gccgcgaaag
      661 gcgaccattc ccctggcgct ggttgaacgg gctgtgctgg aggcgatgca gacgataaca
      721 tcgattgcgg tgatgccccg cttggtcgca tatacatgga atcccgcgcg tagatcgatg
      781 ggtcaaaggg aggcactgga atgccgttgc tgtatggacg cgcatttgtg gcaggcgatg
      841 taccggctga tttacgacgc gccgccaggg agacaaggag tacaatcaat ccgaccaggg
      901 caacgagtac cagtgcgccg cccagtagga tcagcgccca ggagcgatca tcgccgcttg
      961 cgctgccagt gccgttcggg tggctggttg gtcgagcagt aatctgcggt gttggtgtcg
     1021 cctcgccggg aggaagagcg atgcctggct gattcaggtg caggcgccac cggttctcca
     1081 ggtgaatagg atcgataccc agcgcggcca gcatagcctg gttaaagtcc agcgagagac
     1141 tgctcagatc cttgataaac tggatcatct tcggctggcc gaaagtagtg tacatgtatt
     1201 gcaccaggtt ccaactctgc gcataggcca ggtatgcctg gtcggagtcg gcagggaagc
     1261 cgaatgaaat gttgtcgagt cgcaggagcg agtgggtatc gagcgcgcgg ttgaacctgt
     1321 ctatcatagc ctgttcatgg aattgctggt tgaagacggc catgccttca tcgaaccagg
     1381 tcggcacgga agtctgaagg ccgatcaact ggtggaaaac cagatgggtc aattcgtgcg
     1441 gcatatcgcg agacagcgtt gtatcataga tgctcgcgac aacaatcgaa gcttcactta
     1501 gttcgggaaa ggcaataccg ccaacccact cgtaggagcc tggaggaaga gagccttgaa
     1561 aatcctggtc ggttgcgtat acccatagat tgatggaact ctgtaggccg cctcccaggt
     1621 ttccgctgat ccgatcaatg ctcgaactgg cgctgttcag gatgacctga ccgaaatcct
     1681 gcggacgatc gtaccagttt acctgcaaga gtcccctgtt cagatgctgc cacgtgaagc
     1741 gcgtatcgat cgtcgtcagc tgttgctgcg gttggatata ggttgtcccg gcggcatc
//
LOCUS       DASZAK010000686         3551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_336814, whole genome shotgun sequence.
ACCESSION   DASZAK010000686 DASZAK010000000
VERSION     DASZAK010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336814"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1948)
                     /locus_tag="VGT44_12115"
     CDS             complement(<1..1948)
                     /locus_tag="VGT44_12115"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2581588.1"
                     /translation="MSHLSLELLGTPEVRHDGRIVKFRTRKEQALLIYLGVEGGLHSR
                     EKITALFWPDSDEPQGRTSLRRTLADLRSTFYEAGGPAHLIIERDMLGFDFSSDMNLD
                     LRTLENTYSLVRESSTVLEARGTVHHLLLHQLQRAASLYRGPFLEGFSLSEAPNFDNW
                     IGLQREVWHQRMSRILDKLSQVQLDAGELLGAVETLTRWIALDLLNESAQRRLIAVHL
                     AAGDRNAALRAYEVFRLRLAEELNAKPSAETEALLASAYAGSQGKHDQKVGSSSPVHF
                     SAQVEVEEELASPLIGRASEYTRLIEAYRAAEHGQAQVVMLQGEAGIGKTRLAAEFLA
                     WAGAKGADVLPGRAFEAGGRLPYQPLVEALRSRIEQENAPDDLLSDPWLAELSRLLPE
                     LRDRYPDLAVPTGDETAARIRLFEAVVRLGQALAERAPLVLFLDDIQWADAASLDVLH
                     YAGRRWVESKTPVLLLLSLRTEPLTPTSVLSSWLSGMERDVRLTKLTLEALTYDETLQ
                     LIRALGSTNIAALGQWLFAETGGQPFYMMETLKALLERGMLRPRHQMDGSRVIEFVAT
                     PSSVNTLHHFLAPGVHEVIRSRLDQLTPTAFGLLVAGAVLGRTFTFERACQVAALREN
                     VGLPALDEVLRKRLLQEATGEPGEQ"
     gene            2071..2850
                     /gene="ubiG"
                     /locus_tag="VGT44_12120"
     CDS             2071..2850
                     /gene="ubiG"
                     /locus_tag="VGT44_12120"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: protein motif:HMM:TIGR01983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEV2581589.1"
                     /translation="MPVDNELYNRLSSTWWDENEPLNLLRTSLNPGRFGYFRDVLIKQ
                     LKIDTRGKKVLDVGCGGGILAEEFARLGCQVMGIDPSSSSLATARAHAQQASLNIDYR
                     VGVGEDLPFADESFDIVYCSDVLEHVTDLEKVIAEMSRVLKQDGIFLYDTINRTFPSK
                     LVMIKLVQEWKSTSFMPPNLHDWNAFIKPHELLTLMKRHGLDNREKRGLKPGTNPITT
                     TILMRKRKRGKISLYEMGSRMSFQQSKDTSILYMGYAIKAG"
     gene            complement(2880..>3551)
                     /locus_tag="VGT44_12125"
     CDS             complement(2880..>3551)
                     /locus_tag="VGT44_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581590.1"
                     /translation="ITRDRSIRQQRYRCLAVTLAASLVSGFFINFGAGNLAHSLYVPL
                     FAYLVLLASNVYALRQLGSVDRAPEQVRAAPLPWRAALIGALTGLLPLTMILILTLTS
                     STPVPANEYLLLRLFGAILIVLIGAPTPGAVLAVRLLQPMTSPSFLRSSALAGIVMFA
                     GAFVLASLWSIATSSHLLFIDQLKFSGLAFAVGLIALSLVGALRGMLDVRLYWRLTRN
                     KNARP"
BASE COUNT          809 a         1018 c         1005 g          719 t
ORIGIN      
        1 attgctcccc tggctctcca gttgcctcct gtagcaggcg ttttctcaag acttcatcca
       61 gagcgggcaa ccccacattt tctcgtaggg cagccacctg gcatgctcgt tcgaatgtga
      121 aggttcggcc cagcacagcg cctgctacta acagcccgaa ggcggtagga gtcagctggt
      181 cgagtcgtga gcggatcacc tcatgtacgc cgggtgcgag gaagtgatgg agggtgttca
      241 cgctggaagg agtagctaca aactcgatga cccggcttcc atccatctga tggcggggtc
      301 ggagcatccc tcgctcaagc agtgctttga gcgtttccat catgtaaaag ggctgtccgc
      361 ctgtttctgc aaataaccac tgtcccaaag ccgcaatatt ggtactgccc agagcgcgaa
      421 tcagttggag cgtttcatca tacgtgagtg cttccaacgt gagcttcgtt agtctcacat
      481 cgcgctccat gccagagagc cagctggata ggacactggt cggggtaagg ggttcggttc
      541 gcaagctcaa gaggagcaaa acgggtgtct tgctttctac ccagcgtcgt cccgcgtaat
      601 gcagcacgtc gagcgatgca gcgtcggccc attggatgtc gtcaagaaag agcacgagcg
      661 gcgcgcgctc tgcaagcgcc tgacccaggc gcacaaccgc ttcaaacagg cgaatacgcg
      721 cggcggtctc atccccggtc ggcacggcca ggtcagggta acggtctcgc agttcaggca
      781 acaggcgact cagctcggcc agccagggat cgcttagcag gtcgtcgggc gcgttttctt
      841 gttcgatgcg gctgcgtaac gcttcgacaa gcggctgata gggcaggcga cctcctgctt
      901 cgaaagcacg tcctggcagc acgtccgctc ccttcgcccc tgcccaggcc agaaactccg
      961 ctgccaggcg cgtcttaccg atgcccgctt cgccttgaag catgacaacc tgcgcctgcc
     1021 catgctcggc agcacggtag gcttcgatca acctggtata ctcgctcgct cgtccaatga
     1081 ggggactggc taattcctcc tcaacctcaa cctgcgctga gaagtgaacc ggcgaagatg
     1141 accccacctt ctgatcgtgc tttccctgtg atccggcgta tgcactcgcc agcagagctt
     1201 ctgtttccgc cgaaggcttc gcattcagtt cttcggcaag tctcaatcgg aaaacttcat
     1261 aggcccgcag cgcggcattg cggtcacctg ccgccagatg taccgcaatc aggcgccgct
     1321 gggcggactc attcaaaaga tcaagggcta tccagcgagt gagggtctcc acagcgccga
     1381 gaagttcccc cgcatccaac tgcacctgag aaagcttgtc gaggatccgg ctcatacgtt
     1441 gatgccagac ttccctctgc aatccaatcc aattgtcgaa gttgggagcc tcgctgagag
     1501 aaaacccctc caggaaagga ccacgataga gggaagccgc gcgctgaagc tggtgaagca
     1561 agagatggtg aactgttcct cttgcctcca gtactgtgga ggattcgcgg acaagtgagt
     1621 aggtgttttc cagtgtacgc aggtcgaggt tcatatccga ggagaagtcg aagccaagca
     1681 tatcgcgctc gataatgagg tgcgcagggc cgccagcctc atagaaagta ctacgcagat
     1741 cagccagtgt tcttcgtagc gaggtgcggc cttgtggctc atcgctgtca ggccagaaaa
     1801 gcgccgtgat tttctcgcga gaatgcaacc caccttccac tcccagatag atgaggaggg
     1861 cttgctcttt acgagtgcga aactttacta ttcgcccgtc gtgtcgaact tccggggtgc
     1921 caagcaactc caggctcaga tgactcattt cttacccttg ttcacatttc tctcacatat
     1981 ttatcacaac gtgatgatac atttcccaca ggtaactgaa gaacattcta gcgcatagct
     2041 cttggagtga caagaaggag gaatacacct atgccagttg acaacgagct ttacaaccgt
     2101 cttagttcta cctggtggga cgagaacgaa ccgcttaatc tgcttcgcac ctccctcaac
     2161 ccgggccgtt tcggatactt ccgggatgtc ctcatcaaac aattaaaaat cgatactcgc
     2221 ggaaagaagg tattggatgt cggttgtggt ggcggcatcc tggctgaaga gttcgcgcgg
     2281 ctcggctgcc aggttatggg cattgatccg tcatcctcgt cccttgcaac ggcaagggcc
     2341 catgctcaac aggctagcct caatatcgat taccgggtag gagtcggcga ggacctccca
     2401 tttgccgacg agtctttcga catcgtgtac tgcagcgacg ttttggagca cgtgaccgat
     2461 ctggaaaaag tcatcgccga gatgtcccgt gtcctaaaac aggatgggat cttcctctac
     2521 gacacgatta accgcacatt cccaagtaag ctggtgatga tcaagcttgt tcaggaatgg
     2581 aagtcgacca gcttcatgcc tcccaacttg catgattgga atgcgtttat caagccgcat
     2641 gaactgttga cgctcatgaa gcgccatgga ctcgacaacc gggagaaaag ggggttgaag
     2701 cccggaacca accccatcac aacgaccatt ttgatgcgca agcgcaaacg cggtaaaatc
     2761 tccctctatg aaatggggtc acgcatgagt tttcaacaga gcaaggatac ctcgatcctg
     2821 tatatgggct acgcaatcaa ggccgggtaa gaagattttt caagcatcaa ctggcgagat
     2881 cagggacgag cattcttgtt gcgtgtcagc cgccaataca ggcgcacatc cagcatcccg
     2941 cgcagcgcgc caaccaggct gagcgcgatc aggccgaccg caaaggccag cccggagaac
     3001 ttcaactggt cgatgaagag cagatgactg gatgttgcga tgctccacag cgaggccagc
     3061 acgaaggctc ccgcgaacat cactatgccg gctagcgcgc tgctgcgtag aaacgatggc
     3121 gaggtcatcg gttgcaagag tcgcacagcc agtaccgcgc ccggcgtcgg cgcgccgatc
     3181 aagacaatca gtatcgctcc aaacaggcgc aggagcagat actcgttggc cgggacggga
     3241 gttgagcttg tcagcgtcag gatcaggatc atggtcaggg ggagcagccc cgtcagcgcg
     3301 ccgatgaggg cagcgcgcca gggcagcggc gcggcgcgca cctgctccgg cgcgcgatca
     3361 acgctgccaa gctggcgcag ggcatagacg ttgcttgcca gcagcacaag ataggcgaac
     3421 agcggtacat agagactgtg agcgagattc cctgcgccaa agttgatgaa gaagccgctg
     3481 actagagaag cggccagcgt aacggccagg cagcgatagc gctgttgccg gatagacctg
     3541 tcgcgcgtga t
//
LOCUS       DASZAK010000687         2677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_692671, whole genome shotgun sequence.
ACCESSION   DASZAK010000687 DASZAK010000000
VERSION     DASZAK010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692671"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(275..589)
                     /locus_tag="VGT44_12130"
     CDS             complement(275..589)
                     /locus_tag="VGT44_12130"
                     /inference="COORDINATES: protein motif:HMM:NF023178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HEV2581591.1"
                     /translation="MLFTINVTTLLIWLACHFVGDFAFQSTWMSIEKGKSWEVNFYHC
                     ATYTAVFVLFAHSSILATIIIFATHFVIDPLKARYKLIGPIWVDQLLHLLTIVLILGL
                     HL"
     gene            complement(691..2424)
                     /locus_tag="VGT44_12135"
     CDS             complement(691..2424)
                     /locus_tag="VGT44_12135"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV2581592.1"
                     /translation="MSSKELASASSDAPVSQPVDQPANQGEQDIQSSQPPDLPVKESP
                     SDSPPADRRQPVWKRSPFWRDRLVEAGMVCSMAAYYVVGNNYLGASKLFHLNPFVSLP
                     FLLIFAVLSWYRLSFAVALLPMAVPYYFLEKPVYSHYEFELAEIALGVCVGIALIQVF
                     LRGKHWTYWLSWREMRARVGLFVLPIAIFVVAAGVSVLISFAHKDALRAFREEVFDPL
                     VFLLLALYCLRERQDVTRLLLALFGAAFLIALEGVAQYTLFKHTLALDSDGRVHAIFG
                     SANNIGLFFDYSLPIGLALVIIGRRNLSNSLSFLRRWGARLLVLAMLLPMLAVLYLSQ
                     SRGSWVAIALAAILIVLLALRSRKLFIISGLALLAVCVAVAAFFHQQIIDFFVLGHVD
                     TAGISTVTKRIYLWESALRIIRQYPVFGVGLDNWLCYYSRNTICVIPSLHQHYWITLV
                     PGTNIPTGLNEEPNLSHPHNIFLHVWVSIGIFGLLAFIALIALFYRLFARVLKTVRAL
                     GSAAHPDLEWMVIGVGAAMLAGLIQGQVDSAFLAQDMAFCFWMLVTALLLLRVLTNTP
                     WRGRIQSVQQE"
BASE COUNT          664 a          696 c          808 g          509 t
ORIGIN      
        1 ttaaatattt tagggcatat gcctgtgtag gtgccggttt accgcgcaca ccgccgattt
       61 atcggccccg ttgggcatat acaacaagca tcctggccga taatggtttt taacttatta
      121 atacagcaat gtttacgctc aagatagccg gattaatagg ttaaaaacca taatcggcgg
      181 tgtgcgcggt aaaccggcac ctacgagtac atcctgttaa ttatttaatg ttcattctcg
      241 cattcacacg accgctatgc gcttatatcg tacatcataa atgcaggccg agaatcagga
      301 cgatcgtcag caagtgcagg agttgatcga cccagattgg tccgatgagc ttgtagcggg
      361 ccttcaaggg atcgatgacg aagtgtgtgg cgaagatgat aatggtcgcg aggatggacg
      421 aatgcgcgaa gaggacgaag acggcggtat aagtggcgca gtgatagaag ttgacctccc
      481 agcttttgcc cttttcaatg ctcatccacg tgctttgaaa ggcaaagtcc cctacaaaat
      541 ggcaggccaa ccagatgagc agcgtcgtga cattgatggt aaacagcatc ggcgcctcct
      601 tctcatgtat gttgtctcac tttgagtata gggtatgcga agcaaacgaa ctatgaagta
      661 gtgcgttgtc agggtgtgag gaaggtcaca ttattcctgc tgcaccgact ggatacgtcc
      721 gcgccagggc gtattcgtca acacgcgcaa taacagcagg gccgtgacca gcatccagaa
      781 gcagaaggcc atatcctggg ccagaaatgc gctatcgacc tgcccctgga tcagcccggc
      841 cagcatggcc gcgccgacgc cgatcaccat ccattcgagg tctggatggg cggcgctgcc
      901 cagggcgcgc accgttttca ggacgcgggc gaagaggcgg tagaagaggg caatgagggc
      961 gatgaaggcc agcagtccaa agataccgat actgacccac acgtgcagga agatgttgtg
     1021 tgggtgcgag aggttcggtt cctcgttcag gccggttgga atgttcgtgc cgggtacgag
     1081 ggtgatccag taatgctggt gcagggatgg gatgacgcaa atggtgttgc ggctgtagta
     1141 gcagagccag ttgtccaggc caacgccaaa gacggggtac tggcgaatga tgcgcagcgc
     1201 gctctcccac aggtagatgc gcttggtgac ggtgctgatg cccgccgtgt caacgtgtcc
     1261 caggacgaag aagtcgatga tctgctggtg aaagaacgcg gcgacggcga cgcacactgc
     1321 cagcagcgcg aggccggaga tgatgaacag cttacgactg cgcagggcca gcagcacgat
     1381 gaggatggct gcaagggcga tggcaaccca cgagccgcgc gattggctga ggtagagtac
     1441 cgccagcatg ggcagcagca tggccagcac gagcaggcgc gcgccccagc gccgcaggaa
     1501 actgaggcta ttcgagagat tgcggcgacc gataatgacg agcgcgagac caatcggcag
     1561 cgaatagtcg aagaagaggc cgatgttatt cgcgctgccg aaaatggcgt ggacgcgccc
     1621 gtcggaatcg agcgcgagcg tatgcttgaa aagcgtatat tgcgccactc cctccagcgc
     1681 aatcaagaat gccgccccga acagggccag cagcagccgc gtgacatcct ggcgttcgcg
     1741 caggcaatac agggccagca gcagaaagac cagaggatcg aagacctcct cgcgaaatgc
     1801 gcgcagcgca tctttatgcg cgaaagatat caggaccgag acgcccgccg ccacgacaaa
     1861 gatggctata ggaagtacaa atagtccaac gcgcgcgcgc atttcgcgcc aggagagcca
     1921 ataggtccag tgcttcccgc gcaagaagac ctggatcaac gcgatgccca cgcacacacc
     1981 cagcgcgatt tcggccagtt caaattcata atggctgtag accggctttt ccagaaaata
     2041 atacggcacg gccatcggca gcagcgccac cgcgaaagac aggcgatacc agctcagcac
     2101 ggcaaagatc aacagaaagg gcagtgaaac gaatggattg agatgaaaga gtttgctcgc
     2161 gcccagatag ttgttgccca cgacgtaata ggcggccatt gaacagacca tacctgcttc
     2221 gaccaggcgg tctcgccaga acggtgagcg cttccatacc ggctgcctcc ggtcggcagg
     2281 gggcgagtcc gatggagact cttttaccgg caagtccggc ggctggctcg attgaatatc
     2341 ctgttcaccc tggttggcgg gttgatcgac tggttgactg accggcgcat cgctcgatgc
     2401 tgaagcaagc tccttgctac tcatctcaaa acttcacatt tccgggctat cgccaaaaag
     2461 cccataaaaa ttcccacgcc atagagagac gtgtacttat atgacgggag aaacgagcgt
     2521 aaggttgcga tgatagtact tcttgaggtt agcgcaccag ttccctgacg aattgctgga
     2581 agacgtggac gtggtggtca atatcttcct gggtgtgctg gatggagacg gtccactggt
     2641 cgtcgaagcc gggcgggaag aggatgccgc gattggc
//
LOCUS       DASZAK010000688         8535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_52150, whole genome shotgun sequence.
ACCESSION   DASZAK010000688 DASZAK010000000
VERSION     DASZAK010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8535
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52150"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="VGT44_12140"
     CDS             <1..752
                     /locus_tag="VGT44_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581593.1"
                     /translation="VALAVLALMFMALKATATMSPDRRELFTRRWKPALFTGIIASLS
                     SLLVILAFYGPFFIGHSLPAIAQSFTSPPSSQTAHKSILDGIVQTFILNPNPPHTWFY
                     TLLKQLDSAKLWDLINVVTLLASIIIGAIWLWRKPTTRTFILASLLTLSAFLLVAPWF
                     LPWYATWLVALAVVCLPASNQPVARGLIAFALTFSLSVFILYLYNGIPPANVWNLQSC
                     LLTFGPPLLAFIIALLPWPYRRNAVIHHLSP"
     gene            975..2153
                     /gene="tgt"
                     /locus_tag="VGT44_12145"
     CDS             975..2153
                     /gene="tgt"
                     /locus_tag="VGT44_12145"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV2581594.1"
                     /translation="MSPAFSFRIEAECPQSWARAGTIQTPHGAIATPIFMPVGTQATV
                     KSVSPEDLRAAGAQIILANTYHLMLRPGSQLIQEFGGLHSFMHWDGPILTDSGGFQVF
                     SLGHLRKLSEEGAAFKSHLDGSAHMLTPENVMQIEAELGADIILPLDICAAYPCAPSD
                     AREAMLRTHRWAERALIAKELHRPEQTLFGIVQGAFEPELRQESARYIGGLPFPGLSI
                     GGLSVGEPKAMMWEMLRCVTPALPREKPRHLLGVGSPEDLVIGVGMGMDMFDCALPTR
                     VARHGGLFTHSGRVNVKGARFRAEHGPIEEGCDCHTCLNYSAAYVHHLLRAEEQLYYR
                     LATIHNIRFMLRLAGDLRAAILAGSYPAFRDDFLARYIPASEKVRDEQREKWRAARLH
                     "
     gene            2163..2987
                     /locus_tag="VGT44_12150"
     CDS             2163..2987
                     /locus_tag="VGT44_12150"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HEV2581595.1"
                     /translation="MPEPYRFLNTHFLDAATNMAIDEAVLLHHLRGEAPPTLRVFRWS
                     TPSISLGRFQSIEREILADACQREGIALVRRPTGGRAVYHRDEFTYSIVTGKRHGVPS
                     GIVPAYAYLAQGLLAALDQLGIHAELSDERVSKHPSAACFASSTQADLTSGGFKLIGS
                     AQVWKDDALLQQGSLPLDDRSAEFFNLLRYPDVTSRAESLALYREKTTPLHTFAPHVT
                     WDDVAHAFREGFSAALNAEFIPGELSASEWDLARQLVEEKYSKLEWRKERVKLIEG"
     gene            3234..3926
                     /locus_tag="VGT44_12155"
     CDS             3234..3926
                     /locus_tag="VGT44_12155"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2581596.1"
                     /translation="MARKPALPGVDRRQQLLEAALEVFAEQGFEAATNKAIAERAGVN
                     QGLIYFYFASKADVYFATFAHFTDQVMAQVDAVFAQEDDANAADDLARRLRQLLRILS
                     SPPAIHLLRFIYQAISGRAPTGELSSNEGRKVVITLMRHLSQRLRQYLETRMARGEFA
                     TLEAGMVANLMTRTLIITIAGGRGQGGNLVPDLDRLADTMAALYCYGLIPREGNASVL
                     TSLDDRTGSSSP"
     gene            4402..5352
                     /locus_tag="VGT44_12160"
     CDS             4402..5352
                     /locus_tag="VGT44_12160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2581597.1"
                     /translation="MIESPMNVVSSMETGSQVPAIELQTVSKTFGSVQALSEVSFTIP
                     QGQVVALLGPNGAGKTTAISLMLGLRRPSHGQVLLLGRDPRDRRVRSRCGIMLQESGV
                     PLTLTVRETITLFQSYYPRPLPLAQTLEVAGLKKKATAQVGTLSGGQRQRIYFALAIC
                     GDPHVLFLDEPTAGLDVEARHSFWEQIRSFVQQGKTILLTTHYLEEADALAERIIIID
                     HGHVRADGSPAALKAQVANKRVSFDISAPLHPDLFAQLPVQQLEVTEQHVTLLTTQLE
                     SVLRAIFTQPVEITNLEISGAGLEEAFFQLIQDQSEQKGA"
     gene            5359..6174
                     /locus_tag="VGT44_12165"
     CDS             5359..6174
                     /locus_tag="VGT44_12165"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2581598.1"
                     /translation="MPAQTTTARPLAPIGPMLWRQLQAELLRLWRTPSFLVPSLILPL
                     VLYTLLGGLGKGGNRLEDGVSAHIYALASVGTYSIIGVMLFSFGVSIANERAQRVNVL
                     MRATPLPASVYLLAKVLSALLSALMMLLVLCGFAIVVGGVQLSVTAWITLIASLVLGV
                     LPFILFGFAIGNVVNPTGAAPIINLSFFVLAFASGVFVPLSQLPDVLQNIAPIRHSID
                     WPSLPGTPSAYKPVQLPKPYGCSPSMALSSSAWQSTPTAQSNGTLSDKDYCLS"
     gene            complement(6178..6801)
                     /locus_tag="VGT44_12170"
     CDS             complement(6178..6801)
                     /locus_tag="VGT44_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEV2581599.1"
                     /translation="MTQTFDKLPVVAPANNVPGPKQGFWTYDDYAAIPEDGKRYEIVN
                     GVLYMSPAPSWSHQEIVGEIFAHLRAYIRTTGSGGVFIAPIDVELAPNTVFQPDVVVL
                     LKASHKKLKERHIVGAPDVVVEVVSPGSETHDRHRKIDAYARAGVPEYWIVDPDARTV
                     EVLILDKGEYHSQGVYRGQATLPSQIVSGLLVHVEQFFASVWEKRNE"
     gene            6991..7653
                     /locus_tag="VGT44_12175"
     CDS             6991..7653
                     /locus_tag="VGT44_12175"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEV2581600.1"
                     /translation="MSISIEVARRVMEACKRRAHELRSPVSIAIVDAGGHLVLFERMM
                     APYGWATGNISIAKASTAVMFNQSTDAVAQWGSGIPGFAASMASMSQGKFIMAAGGWP
                     IRQGGTTVGGIGVSGGNAPGRDDDIARAGLAALEAAMAPHSSYAPPAQPGQAQQPLQP
                     APAYPTISQAQAIPLQQGASFSAPKQPVGSTYLDNNEDYVSGDEQILFDDEPDLSRGG
                     QP"
     gene            7650..8504
                     /locus_tag="VGT44_12180"
     CDS             7650..8504
                     /locus_tag="VGT44_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581601.1"
                     /translation="MNQQTNNGATPDEQQSGKSATHRRGNRKAGNRPVLITSTEGEQS
                     APDQMDASEMSTAAVATATTTTSSISDAADAPVKKSRPKFFSTIGKKAETSETITVDP
                     AAARLSRATRSAAKPAEKKSSQEANKPAAKAIAPAKSSPSRASAPPARRPSGFKPKHL
                     IGILLYLLVADVAGLWEKSLLGANDKLLFVIGPVQVTLSTVLFLLTLVVLLIVLARFD
                     LVPRSLAPASQSRTTGKGSSQSSSSSNSGDDTTQPGLKQGVKGADDSLYQEYRQNQRY
                     WQRKDRKR"
BASE COUNT         1846 a         2694 c         2261 g         1734 t
ORIGIN      
        1 tggtcgccct ggctgttctc gccttgatgt tcatggcact gaaagcaaca gccaccatgt
       61 cacccgaccg gcgcgaactg ttcacccgac gctggaagcc cgcgcttttc accggcatca
      121 tcgccagcct cagcagcctg ctcgttatcc tggcattcta tggcccattc ttcatcggtc
      181 acagccttcc ggccatcgcg cagagcttta catcgccccc gtcatcgcaa acggcacaca
      241 aatccatcct cgacggcatc gtccagacct tcatcctcaa tcccaatccg cctcacacct
      301 ggttctatac cctgctgaaa caactggata gcgcaaagtt gtgggatctc atcaatgtag
      361 tgacgctgct ggccagtatc atcatcggcg caatctggct ctggcgcaag ccaaccacgc
      421 gcaccttcat cctggcctcg ctcctgacgc tcagcgcgtt tctgctggtc gcgccctggt
      481 tcctgccctg gtatgcgacc tggctggtcg ccctggccgt tgtctgcctt ccggcatcaa
      541 accaacccgt cgcgcgtggt ctgatcgcct tcgccctgac tttctcgctc tccgtcttca
      601 tcctctatct ttacaacggc ataccgccag ccaacgtctg gaacctgcaa agctgcctgc
      661 tcaccttcgg ccccccgctg ctagccttca taatcgccct gctcccctgg ccctatcgta
      721 ggaacgcggt gatacatcat ttgtcaccct gagcgcagcg aagggtctcg ctcgatggca
      781 gtgagatact tcgcttcgct cagcatgaca tgaccggtgt tggttggtaa aattcatcat
      841 cggcgcctgc tgatgtactt tcctgtttgc caacttgacg catatgcgaa aatacaaaca
      901 cagaaaccag gggcaggaag ggcataccct tgcggtcgcc ctctatgtat gaagacccag
      961 atcaacagga atccatgtca ccagcctttt catttcgcat cgaggccgaa tgccctcaga
     1021 gttgggcgcg cgcgggaacc atccagacgc cgcatggcgc gatagccacg cccatcttca
     1081 tgccggtcgg cacacaggcc accgtcaaga gcgtctcgcc cgaagacttg cgcgcggcag
     1141 gcgcgcagat catcctcgcc aacacctatc atctcatgct gcgacccggt tcgcaattga
     1201 tccaggagtt cggcggcctg cattcgttca tgcattggga tgggccgatc ctcaccgata
     1261 gtggcggctt ccaggtcttc agcctgggcc acctgcgcaa gttgagcgag gaaggcgccg
     1321 ccttcaaatc gcacctggac ggctcggcgc acatgctcac acccgaaaac gtcatgcaga
     1381 tcgaggccga gctgggcgcg gatatcatcc tgccgctcga tatttgcgcc gcctatccct
     1441 gcgcgccctc agacgcgcgc gaggcgatgc tgcgcacgca ccgatgggcc gaacgcgccc
     1501 tgatcgcgaa ggagctacac cggccagagc aaacgctctt cggcattgtg cagggcgcgt
     1561 tcgagcctga actacgtcag gaaagcgcgc gctacatcgg cgggctgccg ttccccggcc
     1621 tctccatcgg cggcctatcg gtcggtgaac caaaggccat gatgtgggag atgctgcgct
     1681 gcgtcacgcc cgcgctgcca cgcgagaagc cgcgccacct gctcggcgtc ggctcgcccg
     1741 aagacctggt gatcggcgtc gggatgggca tggatatgtt cgattgcgcg ctgccgaccc
     1801 gcgtggcgcg acacggcggc ctgttcactc actccggtcg cgtcaacgtc aagggcgcgc
     1861 gtttccgcgc tgaacacggc cccatcgagg agggttgcga ctgtcacacg tgcctcaact
     1921 acagtgcggc ctacgtgcat cacctgctgc gcgccgagga gcaattatac tatcgtttag
     1981 ccactatcca caacattcgt tttatgctgc gcctggccgg agacctgcgc gccgccattc
     2041 tcgcgggcag ctacccggcc ttccgcgacg acttcctggc gcgctacatc cctgcgtcag
     2101 aaaaagtgcg cgacgaacaa cgcgaaaaat ggcgcgccgc gcggctgcat tgaggataaa
     2161 ttatgcccga accctaccgt ttcctgaaca cacacttcct ggacgccgcc acaaatatgg
     2221 caattgacga ggccgtcctg ctgcatcacc tgcgcggcga agccccgccc accctgcgtg
     2281 tctttcgctg gtcaacaccc tccatttcgt tggggcgctt ccagagcatc gaacgcgaaa
     2341 ttctcgccga cgcctgccag cgcgagggca tcgcgctggt gcggcggcca accggcgggc
     2401 gcgccgttta tcaccgcgac gagttcacct acagcatcgt caccggcaaa cgccacggtg
     2461 taccatccgg catcgtccca gcctatgcct atctcgcgca gggactgctt gccgcgctcg
     2521 accagctggg catccacgcc gaattgagcg acgagcgcgt cagcaaacac ccatccgctg
     2581 cttgcttcgc ctcctccaca caggcagacc tgacgagcgg cggattcaaa ctgatcggca
     2641 gcgcgcaggt ctggaaggac gacgccctgc tgcaacaagg ttcgcttccc ctggatgatc
     2701 gctcagcaga attcttcaac ctgctgcgct accccgatgt gacctctcgc gccgagagcc
     2761 ttgccctcta ccgcgaaaag accacgccct tgcacacctt tgccccccac gtcacctggg
     2821 acgatgtcgc ccacgccttc cgcgaaggct tcagcgcagc gctaaacgct gaattcatcc
     2881 ccggcgaact aagcgcctcc gaatgggacc tggcgcgaca actggtggaa gagaagtaca
     2941 gcaagctgga gtggcgcaaa gagcgggtga agttgatcga aggataatct agtgattacc
     3001 cataaatccg caagagatgc ttcgctacgc tcagcatgac agaacctctg gttgtcaccc
     3061 tgagcgcagc gaagggtctc gcttgatggg ctgagagatg cttcgctgcg ctcagcatga
     3121 catgactggt acttacgctt tgatactgcc tgtagatctg atcttgacaa atcacccaac
     3181 accaattatt atttattcga gtaactaatt aatctcatac ggagtaacat acgatggcac
     3241 gtaagccagc cctacctggc gttgatcggc ggcagcaatt actggaggcg gcgctggagg
     3301 ttttcgccga acagggcttc gaggcggcaa ccaacaaagc cattgccgag cgcgcgggcg
     3361 ttaatcaagg gctgatctac ttctactttg cgagcaaggc agatgtctat tttgcaacct
     3421 tcgctcattt taccgaccag gtaatggcgc aggttgacgc cgttttcgcg caggaagacg
     3481 atgccaatgc tgctgatgac ctggcgcgca ggctccggca actcctgcgg atattatcct
     3541 cgcctcccgc tatccactta ctgcgcttta tctaccaggc catcagcgga cgcgccccta
     3601 ccggagaatt gagcagcaac gaaggaagaa aggtggtcat aacgctaatg agacacctgt
     3661 cacagcgact gagacaatac ctggagaccc gtatggcgcg cggagagttc gctacccttg
     3721 aagccggcat ggtagcaaac ttgatgacgc gtactttgat tatcaccatc gccggtggac
     3781 gcgggcaggg cggtaatttg gtacccgacc tcgaccggtt agcggatacg atggccgctc
     3841 tctattgcta cggcctgata ccccgcgaag gaaacgcctc tgtcctgacg tctttggacg
     3901 accgaacagg atcatcgagt ccgtaggggc gcaagtgaac gcccacgagg tcgtcccgcc
     3961 cggcccgctg tcatcctgag cgcagcgaag gatctctcgc gggtctaagc taggtgcaga
     4021 tccttcgctg cgctcaggat gacagctatg cttatgcgat cgaccatggg agccgattct
     4081 gttaccgggc aaataaacga ataatgaagc cattttatac aggcaattag ttactcaagt
     4141 aaataattag taaacgccca tttctgggct gaaaggaaag acaatgcttg gtttactggg
     4201 cttgccgcct cttgtcgtac tcgtgattgt gcttggtatt cttgcgtatg tggtctatca
     4261 tatcattgcc accggaacag tccaacctct gacaatcatt gcgctcatct tgcttggatt
     4321 cgccctcgtg cgaattttgt acagaatgtg gcacaaatca caacagagcg aataagccgc
     4381 gctcacaagg agatcttcac aatgatagaa tcgcctatga atgtcgtcag ttcaatggaa
     4441 actgggtcgc aggtaccagc catcgaactg caaaccgtct caaagacgtt tgggtccgta
     4501 caggctctaa gcgaagtcag tttcaccatt ccccagggcc aggtcgtggc gctgcttgga
     4561 cccaacggag cgggaaagac aacggccatc agtctgatgc tgggtctgcg ccgcccgtcg
     4621 catggacagg tgcttctgct tgggcgcgat ccccgcgacc gccgcgtgcg cagtcgctgc
     4681 ggtatcatgc tgcaagaatc cggcgtgccg ctgaccctca cggtacgcga gaccattacg
     4741 ctctttcaga gttactatcc acgtcccctt ccactcgccc agacgcttga ggtcgccggt
     4801 ctgaagaaaa aagcaacagc ccaggtcgga acgttgtcgg gcgggcagcg ccagcgcatc
     4861 tacttcgcgc tagcaatatg cggcgatccc catgtcctgt ttctggatga gccgactgcc
     4921 ggactcgatg ttgaagcccg tcacagcttc tgggagcaga tacgctcgtt tgtccagcag
     4981 ggcaagacca tcctgttaac cacccactac ctcgaagaag cagacgccct ggccgaacgc
     5041 atcatcatca tcgaccatgg tcatgtgcgc gccgacggct ctccagcagc cctcaaagcc
     5101 caggtagcca acaagcgcgt cagtttcgac ataagcgcac ccttgcaccc tgatcttttc
     5161 gcacaattgc cggtgcagca gttggaagtg acagaacagc atgtcaccct gctgacgacc
     5221 caactggaaa gcgtactgcg cgccatcttc acccagccgg tagaaatcac aaacctggag
     5281 atcagcggag caggtctgga agaagccttt ttccagctca tccaggacca atcagagcaa
     5341 aaaggagctt gagaagccat gccagcacaa accacaacag ccagaccact cgcgcccatc
     5401 gggccgatgc tctggcgtca attgcaggca gaattgctgc gcctgtggcg cacccctagc
     5461 ttcctggtcc catccctgat ccttcctctc gtcctctaca cccttctggg agggttgggc
     5521 aaaggcggaa atagattgga ggatggcgtg agcgcgcata tctacgcgct cgcctccgtt
     5581 ggcacctaca gcatcatcgg tgtgatgctc ttttcctttg gagtgagcat tgccaatgag
     5641 cgagcgcagc gcgtgaacgt cctgatgcga gccacaccct tgcccgcctc ggtctacctc
     5701 ctggccaaag tgctgtctgc gctgctgtcc gccctgatga tgctgctcgt cctctgcggt
     5761 ttcgccatag tggtcggcgg tgtgcaactc agcgtcaccg cctggatcac cctgatcgcc
     5821 tcgctggtgc tgggtgtctt gcccttcatc ctgttcggat tcgccatcgg caatgtggtc
     5881 aatcctaccg gcgcagcccc catcatcaac ctgtcctttt tcgtgcttgc atttgcctcg
     5941 ggggttttcg ttccgctctc gcagcttccc gatgtcctgc aaaacatcgc ccctattcgc
     6001 cattctatcg actggcccag cttgcctgga acgccgtcgg cgtacaaacc ggttcaattg
     6061 ccgaagccat atggctgctc gccctctatg gcgttgtctt cttcggcctg gcagtctacg
     6121 cctaccgcac agagcaacgg cacactttcg gataaggact actgcctatc gtaagcctca
     6181 ttcatttctc ttttcccata cggaagcaaa aaactgctcc acatgtacca acaacccgga
     6241 tacaatctgg gagggaagcg tcgcctggcc gcgatagaca ccctgtgaat ggtactcacc
     6301 tttatccaat atcagtactt caacggtgcg cgcatcagga tcaacaatcc agtattctgg
     6361 tacacctgcg cgagcataag catctatctt tctatgtcta tcatgagttt cgctgccggg
     6421 tgagacgact tcgacaacca cgtcgggagc gccaacaata tgacgctcct tcaatttctt
     6481 atgacttgcc ttcagtaaga caaccacatc gggctgaaat accgtattgg gagcaagctc
     6541 aacatcgatg ggcgctatga atactccacc cgaccctgtt gttcgtatgt aagcgcgaag
     6601 atgcgcgaag atttcaccaa cgatttcttg atgcgaccag ctcggtgcag gcgacatata
     6661 cagcactcca ttcacaattt catagcgctt cccatcttca ggaattgccg catagtcgtc
     6721 gtaggtccag aagccctgct tagggcctgg aacgttatta gcaggggcaa ccactggaag
     6781 cttatcaaaa gtctgagtca tatcggcctg gcctttcgtc taaggtaacg ctacggtcat
     6841 tgtaccatga ggcggcgaaa tagaacagca atcaactgcc tatagccaag cagcccagaa
     6901 tacggtacaa tagcatcaat gtaactctgt ggaattctgg gcggcagagc agatgaagat
     6961 ttcgtctccg tcgtcgtgaa ggagtgagtt gtgagcatat cgatagaggt tgcccgccgc
     7021 gtgatggagg cgtgcaagcg ccgcgcgcat gaactacgca gccccgtcag catcgccatt
     7081 gtggacgctg gcggccacct ggtgttattt gagcgcatga tggcccccta cggctgggcg
     7141 accggcaata tcagcatcgc gaaggccagc accgccgtca tgttcaacca gtctaccgac
     7201 gcggtagcgc agtggggttc cggcatccca ggctttgccg cgagcatggc gagcatgtcg
     7261 cagggcaagt tcatcatggc tgccggggga tggccgattc gccagggcgg aacgaccgtc
     7321 ggcggtatcg gcgtgagcgg cggcaacgcc ccaggccgcg acgacgacat cgcgcgcgcg
     7381 ggtctggctg cgcttgaggc cgccatggct ccgcattcca gctacgcgcc gcccgcgcaa
     7441 ccaggacagg cccagcaacc attgcagcca gccccggcat acccaacaat ctcacaggcc
     7501 caggccattc cattacagca gggagccagt ttttctgctc ccaagcaacc tgtcggcagc
     7561 acttatctcg ataataacga agactacgta agtggcgacg aacagatact gttcgatgac
     7621 gaaccagatt tgtcgcgtgg aggtcaacca tgaaccagca aacaaataac ggcgcgacgc
     7681 ccgacgaaca acagtcgggc aagtcggcga cgcatagacg cggaaaccgc aaggcgggca
     7741 accgccccgt cctcatcacc tcgaccgagg gcgaacaatc agcgccagat caaatggacg
     7801 cgagcgaaat gtccacggcg gcagtcgcga cagcaacaac cacgacatcg tcaatctcag
     7861 acgccgcaga cgctcctgtc aaaaagagtc gtccaaaatt tttctcgact atcggcaaaa
     7921 aagcagaaac aagcgaaacc atcacggtcg acccggcagc cgcacgcctc tcgcgtgcca
     7981 cacgcagcgc ggccaaaccc gccgagaaaa aatcgtcgca agaggcaaat aaaccggcag
     8041 caaaggccat cgcacccgcg aaaagttcgc cttctcgcgc atctgcgcca cccgcgcgcc
     8101 gaccgagcgg cttcaaaccg aagcacctga tcggcatcct gctctacctg ctcgtcgcgg
     8161 atgtggcggg cctgtgggaa aagtcgctgc ttggggcaaa cgacaaactg cttttcgtta
     8221 ttggccctgt gcaggtcacg ctctcaacag tcctcttcct gctcacgctc gtcgtgctgc
     8281 tcatcgtgct ggcgcgcttc gatctggtac cgcgcagcct ggcgcccgca tctcagtcgc
     8341 gcacaaccgg caagggatcg tcgcagtcgt catcctcatc caacagcggc gacgacacga
     8401 cacagccggg tctgaagcag ggagtcaaag gcgctgatga cagcctctac caggaatatc
     8461 gccagaacca gcgctactgg cagcgaaaag ataggaagag atagtctatg tatggtatac
     8521 tcttaataat acgta
//
LOCUS       DASZAK010000689         4580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_622823, whole genome shotgun sequence.
ACCESSION   DASZAK010000689 DASZAK010000000
VERSION     DASZAK010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622823"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VGT44_12185"
     CDS             complement(<1..807)
                     /locus_tag="VGT44_12185"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV2581602.1"
                     /translation="MQAPVTLRRVLTFRTIVSTSTGLAYAAISLLGCVQLASLVGGDS
                     GWIAIVAAGLLAVLAALCFSELNALYPTAAAIRQYMKEAFSENFSLIITFGYLLTIIT
                     VVAADSYIVGSAITYALNLPNWASLVWILALLALATGANLRGIKIAGLLQDITTYSLL
                     AFVIVISLIALAQHHFQLRDPFAALSNPGGLLNAIAVGVFVFSAFEWVTPLSEEISNI
                     RLIPRGMFVALALLGLSYALFTLAASSFLNVHNAAIFNSPVPQMLLGNAVL"
     gene            1031..2056
                     /locus_tag="VGT44_12190"
     CDS             1031..2056
                     /locus_tag="VGT44_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581603.1"
                     /translation="MFSTNDEQHQNRSARLTRLQCAAGVLAITLAAFSLLCIHSFATP
                     VLASAGSGQTAAATTSSQGPLDDDTQTVIPSQTPTGNPSPTVTNTPSATATKTSTPSP
                     TATHPATPVPTSTRPPVATARPSSTVVGKQTPSPVASSISIGAQGAGANVTPVVSVSS
                     AQSNQSNQPTNQPDSISPLIYFAVALACLGLLGMLLKIWWSAMRSWLWYSRPSPKLPS
                     QAKMARNARPTHHQQNPLIAQRTVNLAVPYVPTIPNTPYVLPNPNTPYALQGSAGPNN
                     ISPATPYGIAIANAPTTEHAIATPSGTLFRDYFAAAQRQSQSNQTGNTSVSIDPNAQA
                     LIEPYVY"
     gene            2253..3758
                     /locus_tag="VGT44_12195"
     CDS             2253..3758
                     /locus_tag="VGT44_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2581604.1"
                     /translation="MKKYTHAIFILIACLIFIVPVSFALAAGAHGSHSASPSPFASTS
                     GGQWPSYGYDVAGTRYNSKEKTLKPANVGTMALDWSYKTGGYVQSSPAVVNGVVYIGS
                     NDGYLYAFEASTGALLWSYYSGYYGGFIGSPAVANGVVYAGSNSKEMYALNASTGALI
                     WSYTLGTATTASPTLANGIVYIMDENGVVYAFKARNGNLLWTYTAGQGTYTQHSQVVA
                     NGVLYFGSNDYKLYALNASTGALLWTFATGSYVAVAPAVANGVVYIGSADFKLYALNA
                     TTGALLWSFTTGSYIQPAPAVANGVVYIGSNDDKLYALNASTGALLWTHTAGSYVLNS
                     PLVANGVVYDSSYSDDTVFALNAKTGNLLWSYAVGGPYNDDSPSVANGMLFIGANDYK
                     VYAFALPQPMKVSPGILNFTSPAGGPSPLQQTVTLSNSGTSTLTWSAGAASQLWLTVS
                     PTSGTIAAGFGVSQTFTVDITGLAAGSYTASVVITPSIGSPFTIVVRLTLT"
     gene            complement(3817..>4580)
                     /locus_tag="VGT44_12200"
     CDS             complement(3817..>4580)
                     /locus_tag="VGT44_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581605.1"
                     /translation="TVTIVPTTVTSSVIRTHDLEVVLQVSPGPYFLGEEIEVKLTLTN
                     YSSTAAYNVKSGYDAKDCAPDLANNLAVVMTGSPTLADTTLQHKLAGFDCSDVAQEYG
                     TVDPANPPSYRYTILPVYTTTVLTYSGTVTLATPLRFIDMYTEAYDQPLGGRTPTVQI
                     HVQAKVPPGRLITGQQRDTQVTISAPPPARNQLTYTYKTSCPGNPSWQWEVIQRPPLT
                     LQKPKCISNPSGKVTQWVYVIGAPGYAVFTGSLNG"
BASE COUNT         1053 a         1288 c         1244 g          995 t
ORIGIN      
        1 taagaccgcg ttgcccagca gcatctgggg taccggtgaa ttgaagatag ccgcgttgtg
       61 aacattcagg aagctgctgg cggccagcgt gaagagggca taggagaggc cgagcagcgc
      121 cagggcgaca aacatgccgc gcgggatcag gcgaatattc gagatttcct cggaaagcgg
      181 cgtgacccac tcgaaggccg aaaagacaaa gacgccaacc gcgatagcgt tgagcagccc
      241 gccggggttg ctcagcgcgg cgaaggggtc gcgcagttgg aagtgatgct gcgccagcgc
      301 gatcagcgaa ataacgatga cgaaggccag caggctgtac gtggtgatgt cctgcagcag
      361 gcccgctatc ttgatgccgc gcaggttggc gcccgtcgcc agcgccagca gggccagaat
      421 ccacaccagc gatgcccagt tcggcaggtt taaggcgtaa gtgatggcgc tgccaacgat
      481 gtagctatcg gcggccacga ccgtgatgat cgtcagcaga tagccaaagg taatgatcag
      541 cgagaagttt tcactgaacg cttccttcat atactgccgg atggccgccg cagtggggta
      601 cagagcgttc agttccgaga aacacagcgc cgccagcacc gccagcagac cggcggcgac
      661 aatggcgatc cagccagaat ccccgccgac cagcgaagcc agttgcacac agcccagcaa
      721 gctgatggca gcataagcca ggccggtact ggtggaaaca attgtgcgaa aagtcaggac
      781 gcgacgaagg gttacgggag cctgcataca ttcctcctgg ggcaaattac ccggcgcatg
      841 gtgggtaaga gaagcgtacc ggaaaacaaa tagcttgtca acctcgctgc gttgctagtt
      901 ccggttgagg tcccacagct cgaagcccca aaagcatagt acgaaccgca ttgatgcttg
      961 acggtgcttg attgctcccg ctacactttc atgtaagggg gaatattgca gaaacacgag
     1021 gtaatcagct atgttttcga cgaacgatga gcagcatcaa aacaggtctg cgcggctaac
     1081 tcgcttacaa tgcgccgccg gtgttctggc aatcacctta gccgcattct ccttgctttg
     1141 catccattcc tttgcaaccc cggtactcgc cagcgcgggg tctgggcaga ctgctgcggc
     1201 aacgacgagc agccaggggc cgttagacga cgacacacaa acggtaatcc cctcgcagac
     1261 tcccacaggt aatccatctc caaccgtcac gaacacaccg tctgcgactg cgactaaaac
     1321 gtccacgcct tcgccgaccg cgacccaccc ggcgacgccg gttccgacat ccacccgccc
     1381 gcccgtagca accgcccggc catcgtctac cgtcgttggc aagcaaacgc catcgcccgt
     1441 cgcatcatcg atcagcatcg gcgctcaggg tgcaggcgcg aatgtgacgc ccgtcgtctc
     1501 ggtttcgtcg gcgcaaagca atcaatccaa tcagccgaca aaccagcccg atagcatttc
     1561 tcccctgatc tactttgccg ttgccctcgc ctgcctcggc ctgctcggca tgttgctgaa
     1621 aatctggtgg agcgccatgc gctcgtggct ctggtacagc aggccgtcgc caaaattacc
     1681 ttcccaggca aagatggctc gcaatgcacg ccccactcac catcagcaga atcccctgat
     1741 tgcccaacgc accgttaacc tggctgttcc ctatgttcct actattccca atacacctta
     1801 tgtgctgccc aatcccaata ctccctatgc cctacaaggc agtgcagggc caaacaacat
     1861 atcgccagcc acgccttatg gcatagctat cgccaatgct cccaccaccg agcacgccat
     1921 tgccaccccg tccggcacat tgttcagaga ctattttgcc gccgcgcaaa ggcaatccca
     1981 gagcaatcag acgggcaata catccgtgtc gattgacccg aacgcgcaag cgctgatcga
     2041 gccatacgtg tattgagtcc ggccctacga cgagggctgg atttatttgt taaaactcat
     2101 tatcgacccg gtggcttgat atggataatg caagataagt atatcgaaat gatgcagtcc
     2161 ataggaatat ctcaaccatt tgacaacctg gtcttaacct ctgcctggta aaataggctg
     2221 gttccactta ttttacgaga aagaggtacc aattgaaaaa gtatactcac gctatcttca
     2281 tccttatcgc ctgcctgatc ttcatagttc ctgtttcctt tgctctggcg gcaggcgctc
     2341 atggctctca tagcgcaagt ccgagtcctt tcgcatctac atctggcggt caatggccta
     2401 gttatggata tgacgttgcc gggacgcgct acaactccaa agaaaagacc ctgaagcctg
     2461 ccaatgttgg cacgatggct ctggattggt cgtataagac gggtggctat gtgcagagtt
     2521 ccccggctgt agtgaacggg gtcgtctata tcggctctaa tgatggctat ctgtacgctt
     2581 tcgaggcaag taccggcgca ctgctctggt cgtattattc cgggtactac ggaggtttta
     2641 tcggttcgcc agcagtagca aatggcgtgg tttatgccgg gtctaatagt aaagagatgt
     2701 acgcgctgaa cgcgagtacc ggggcattga tctggtccta tacgcttggg acagcgacta
     2761 ccgcttcgcc aacgctggcg aatgggatag tgtatatcat ggatgagaat ggtgtggtat
     2821 acgcgttcaa agccaggaac gggaacctgc tctggaccta taccgccggc cagggtacgt
     2881 acacgcagca ctcgcaagtc gtggcgaatg gagtactcta tttcgggtcg aatgattaca
     2941 aactgtacgc gctgaatgcg agtaccgggg cgctgctctg gacctttgcc accggcagtt
     3001 atgtagcggt tgcgccagca gtggcgaacg gggtggtgta tatcgggtct gctgatttta
     3061 agctatacgc gctgaatgca accaccggcg cgctgctctg gtcctttacg acgggcagtt
     3121 atattcagcc agcgccagca gtggcgaacg gcgtggtcta catcgggtct aatgatgata
     3181 agctgtatgc ccttaatgca agcaccggtg cgctcctctg gacccatacc gccgggtcat
     3241 atgtgctcaa ttccccactc gtggcgaatg gggtggtcta tgactcctct tacagtgatg
     3301 atacggtctt cgcgctcaat gcaaagaccg ggaatctgct ctggtcgtat gctgttggcg
     3361 gaccatacaa tgatgattct ccatcggtag cgaacggaat gctctttatc ggggctaacg
     3421 attataaggt ctatgccttc gccctgcccc agccaatgaa agtcagccct ggcatcctga
     3481 atttcacctc gccagcaggc ggccccagcc cgcttcagca aactgtcaca ctctcgaatt
     3541 caggaacaag tacgttaacc tggtcggcag gagccgcgtc gcagctctgg ctcaccgtct
     3601 cgccaacatc gggaacgatt gctgccgggt ttggcgtgtc tcagacattc accgtcgata
     3661 tcactggatt agcggcgggg tcctataccg ccagcgtcgt aataacccct tctatcgggt
     3721 cgccattcac catagtggtc agactaacct tgacgtaaat gcacattgaa tcaataacgc
     3781 ggacaccgat gtagtaataa gctctggaac gcctgctcaa ccgttgagac ttccggtaaa
     3841 aacagcgtag ccgggtgccc caattacgta gacccactgc gtaactttgc cggaaggatt
     3901 gcttatgcac tttggttttt gtagtgtcaa aggtggccgc tgaatcactt cccattgcca
     3961 actgggatta cctgggcaac ttgttttgta agtgtaggtc aactggtttc gcgcaggggg
     4021 cggagcactg atggtcacct gggtatcgcg ttgttgaccg gtgatcaaac gacctggggg
     4081 aaccttcgcc tggacatgaa tctgaacagt aggagtccga ccgcctaatg gctggtcata
     4141 agcctccgtg tacatgtcta tgaatcgcag cggcgtggca agcgtgacgg tgccgctgta
     4201 tgtgagaacg gtggtggtat agaccgggag gatcgtatag cgatacgaag gggggttagc
     4261 aggatctacc gtcccatatt cctgtgccac gtcactacaa tcaaaccctg ccagtttgtg
     4321 ttggagcgtc gtatcagcaa gagtggggct gccagtcatt acaacagcaa ggttatttgc
     4381 taggtctggc gcacaatctt tcgcatcata tccactcttc acgttatagg cggcagtgga
     4441 cgaatagttg gtgagcgtga gcttcacctc tatctcttcc ccgagaaagt acgggccagg
     4501 agaaacttgc agaactacct ctagatcatg cgtccgaatt accgaagaag tcaccgttgt
     4561 tggcacaatc gtcacggttg
//
LOCUS       DASZAK010000690         4505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_657638, whole genome shotgun sequence.
ACCESSION   DASZAK010000690 DASZAK010000000
VERSION     DASZAK010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657638"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..434
                     /locus_tag="VGT44_12205"
     CDS             78..434
                     /locus_tag="VGT44_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2581606.1"
                     /translation="MLTHVPQATALKAKLFRGFSDMSRLAILEALRDGPLTVSALVEK
                     TSLSQSNVSNHLRCLSDCDLVVSTPQGRYTLYQLSDTRVAALLELADELLSDVARGVY
                     ECTRYEPLETSGKEAD"
     gene            436..2547
                     /locus_tag="VGT44_12210"
     CDS             436..2547
                     /locus_tag="VGT44_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV2581607.1"
                     /translation="MARTKLEIPVHGMDCAECTMHVQHALAALPGVEDVHVLLSSEKA
                     VLQIDPSQVDLPTIRKAVEGVGYTVPEQAASLKEQTASKPLATFTRPIFTLFGLVFGA
                     VLLVVIVGEWLGLFKRVTDWVPWYIGWGLVLLAGAPIFWNVIRATLRRQVISHTLMSI
                     GVIAALVVGQWPTAMVIVFFMRVGDYAEHFTTERSRQAVRRLTALAPQTARVERDGVE
                     QVVPIEKVQVGETVIVRPGESIPVDGEVTSGQATVNQATLTGESMPVEVSSGGRVYAA
                     TIATLGSIRLRVTHVGADTTFGRVITMVEEAEAHRAPVQRIADRFSAYFLPVVAAIAA
                     ATFLASRNPLAVAAVLVVACSCSFALATPIAMLASIGAAASRGILVKGGKYLESLSHA
                     DVVLIDKTGTITTGQPQISDVLALAGHTQDEVLALAASVERDSEHPLAEAVRQAASKR
                     ALSLYEVEHFESVPGLGVRAEVNGDPIVVGNARMLASTAPAHVTQALERQGKTLLLVE
                     QAGTLIGVLAAADTIRPEVPAAIERLRTPAISTIELLTGDNEQTAEAQATALRITYRA
                     ELLPEDKIRIVKEYQAEGHTVVMIGDGVNDAPALAQADVGIAMGVMGSDVALEAAHVA
                     LMREDWNLVPDLFVIAKRTMRVVKMNLTFTIVYNLLGLSLAAFGILPPILAAAAQSLP
                     DLGILANSSRLLRQKMPTQRS"
     gene            complement(2544..2990)
                     /locus_tag="VGT44_12215"
     CDS             complement(2544..2990)
                     /locus_tag="VGT44_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581608.1"
                     /translation="MTQENSTSESTSDEAPKWLTPGVQGIGVASFLSDLGHEVPTSLF
                     PSLLINMLGASAAALGLVEGLADGLAGLARLLGGSLADDPDRRRRTAIGGYTSTAILS
                     ALIGAAGAVWQVAVLRMAAWASRRLRVLARNALLADMVPTSVYGRA"
     gene            complement(3031..>4505)
                     /locus_tag="VGT44_12220"
     CDS             complement(3031..>4505)
                     /locus_tag="VGT44_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581609.1"
                     /translation="ADRLIPRGLIGCSGKILGCRALVFLHDEQGHPRLAMTHRGDQHL
                     TMGLPPILARYQDCEEEAPSVRVIVDREGMAAPFLRDLKALGHTVLTVLRTDQYDGLE
                     SFTEVGMFVPLERDRHGKLVREVAPACFALPLPDQKGQFLPLRVALIRDLRRHVPCTR
                     QEEETDEDVRKPAWWQAEWKAEPTPATRTTAKLIPIVTTADTVDAVELAQTYTRRWPV
                     QENIIRDFLVPLGLDTNHGYGKRPIVNSEVAKKRAALEKRFSNVERWMASARVRSHKA
                     SLLYDRLWKQANAYGDELYCQLNAHQDALAAQGVNYYERRAQIKAEKAAIDAELEQRW
                     QRVWRAYNKSSKEGEKQERYARQQCDLLRALEDLAATERTMYELDNRKDQVMTICKLA
                     LANVVMWTRDHYFPTTYAHATWARLAPFFQLRGVVVSTQHTVSVELCPFNDRQYNRDL
                     LALCQRVNERHVRLPDGRLLLFSVKEICRPILHGQQRPIA"
BASE COUNT          945 a         1289 c         1287 g          984 t
ORIGIN      
        1 tgcgtaagca taagatggtc cctttcatat tatcatatca ttaattaatg atatgataat
       61 atgaaaggga ccatcttatg cttacgcacg taccccaggc aacagcgctc aaagccaaat
      121 tgttccgtgg cttttcggat atgtctcgac tcgcgatctt agaagctttg cgcgatgggc
      181 cactgaccgt cagcgctctc gtggagaaaa ccagcctcag tcaatcgaac gtctcgaacc
      241 acctgcgctg tctgtcggat tgtgacctgg ttgtctccac cccccaggga cgctacacgc
      301 tgtaccaatt gagcgatacg cgggtcgctg ctttgcttga acttgctgat gaattactga
      361 gtgacgtggc acgaggtgtt tacgaatgca cgcgctacga gccgcttgaa acttctggaa
      421 aggaagcaga ctgagatggc tcggacaaag cttgaaatcc ctgtgcatgg gatggattgc
      481 gcagaatgca cgatgcatgt gcagcatgcc cttgcagctc ttcccggcgt tgaagatgtc
      541 catgtgctgc tttcctctga aaaggctgtg ctccagatcg atccatcaca ggtcgatctc
      601 cctaccattc gcaaggcagt cgagggagtg ggctacacgg ttcctgaaca agctgcctct
      661 ctaaaagagc agactgccag caaaccactc gcgacgttca ctcgtcctat ttttaccctt
      721 ttcggccttg tctttggggc agtactgctc gtagtgatcg tgggggaatg gcttggctta
      781 ttcaagcgag tgacagattg ggttccctgg tatatcggct ggggtctcgt gctgctggca
      841 ggcgctccga tcttctggaa tgtcatccgc gccacactcc gccgccaggt catttcgcac
      901 acccttatga gcatcggggt catagcggcg ctggttgttg gacagtggcc gacggccatg
      961 gtgatcgtat tcttcatgcg ggtcggcgat tatgctgaac actttacgac cgagcgttcg
     1021 cgccaggcgg tcagacgttt aacggcgctt gctccccaaa ccgcgcgggt agagcgcgat
     1081 ggggtagagc aggtcgtacc tatcgaaaag gtgcaagttg gtgagacggt gatcgtccgc
     1141 ccaggcgagt cgatcccggt cgatggagag gtcaccagtg ggcaggcgac cgtcaaccag
     1201 gcaacgctta ccggggagtc catgccggtc gaagtcagca gcggaggtcg cgtctatgcg
     1261 gcaaccattg ccacacttgg cagtatccgg ctcagggtca cgcatgtagg agcggatacc
     1321 accttcggtc gagtcatcac gatggtagag gaggccgagg cacatcgagc gccggtgcag
     1381 cgcatcgcgg accgcttctc ggcctacttt ctgccagtcg tggcagccat tgcagcggcc
     1441 actttcctcg cgagccggaa tccactggct gttgcagcgg tgctcgttgt cgcgtgttcc
     1501 tgctcgttcg cgcttgcaac gccgattgcc atgctcgcct ctattggggc ggctgcctct
     1561 cgtggcatcc tggtcaaagg aggaaaatac ctggagtcgc tctcgcatgc cgacgtggtg
     1621 ctcatcgaca agaccggcac aatcaccacc ggccagcccc agatttctga cgtgctcgcg
     1681 cttgccggtc acacccagga cgaggtgctc gcgctggcgg caagtgtcga gcgagactca
     1741 gaacatccgc tggctgaagc cgtgcgacag gccgcttcta aacgcgcgct ttcactttat
     1801 gaagtggagc attttgagtc tgtcccaggt ctcggcgtgc gagccgaagt gaacggggac
     1861 cctattgttg ttgggaatgc acgtatgttg gcatcaaccg ccccagcgca cgtcacgcag
     1921 gcgttagagc gacaaggaaa gacgctgctc ctggtcgagc aagctggaac gttgatcggg
     1981 gtgctggctg ccgccgatac catccgccct gaagtgcctg ccgcaattga gcgtctgcga
     2041 acgcctgcta tcagcacaat tgaactgctc accggcgaca acgaacagac ggcggaggcg
     2101 caggccacag cactacgcat cacctatcgc gcggagctac tgccggaaga caaaatccgt
     2161 atcgtcaaag agtaccaggc agagggccat accgtcgtga tgatcggtga tggggttaat
     2221 gatgctccag cgctcgcaca ggctgacgtg ggtatcgcga tgggcgtgat gggatcagac
     2281 gtagcgctag aggccgccca cgtggcactc atgcgcgagg actggaacct tgtgccagac
     2341 ctgttcgtga ttgcgaagcg taccatgcgg gtagtcaaga tgaaccttac ctttactatc
     2401 gtctacaacc tgcttggtct ttcgctcgcg gcctttggca tactcccacc gattctagcg
     2461 gcggcagcgc aatccctgcc ggatcttggc attcttgcca actcgtcgcg gctgcttcgg
     2521 cagaagatgc caactcagag aagttaggcg cgtccgtaga ccgacgtggg caccatatca
     2581 gccagcaatg cgttgcgtgc aagtacgcgt aatctgcgtg aggcccatgc cgccatgcgc
     2641 aagacggcga cttgccacac ggctcctgct gcaccaatga gagcagacaa aattgccgtt
     2701 gaggtgtaac cgccaatcgc tgttcggcgt cttctgtcag ggtcgtccgc cagcgaacct
     2761 cccagcagac gtgccagccc tgccaggcca tcggcaagac cttctaccag tccaagtgca
     2821 gcagcagacg cccccagcat attgatgagc aaactgggga acagagatgt ggggacttcg
     2881 tggccaaggt ccgacagaaa actggccacg ccgatgcctt gtacaccagg cgtcaaccac
     2941 tttggtgcct cgtcgctcgt cgactcagaa gtcgagtttt cttgggtcat acctcctcta
     3001 atacgacttt tggaatattc aatcagggtg ttaggcaatt ggtctttgct gcccatgtaa
     3061 aatgggacgg cagatctcct tcaccgagaa cagcagaaga cgtccatcag gtaggcgtac
     3121 gtgtcgctcg ttgacgcgtt gacagagggc aagtagatcg cggttgtact gccggtcgtt
     3181 aaaggggcat agctcaacag agaccgtatg ctgcgttgag acaaccactc ctctgagttg
     3241 gaagaaggga gccagccgtg cccaggtggc atgggcatag gtagtgggaa agtagtgatc
     3301 gcgggtccac atcaccacat tggccaaagc gagcttacag atggtcatga cttgatcttt
     3361 gcgattgtct agttcgtaca tagtccgctc cgtggctgcc aaatcctcca aggcgcgcaa
     3421 aagatcacac tgttggcgcg catagcgctc ctgcttctca ccttccttac tgctcttgtt
     3481 gtacgctcgc cagacgcgtt gccagcgttg ctctagttct gcatcaatag ccgccttctc
     3541 tgctttgatc tgtgcccgcc gttcgtaata gttcacccct tgcgcagcga gggcgtcctg
     3601 atgggcattg agctggcaat acaactcatc gccataggca ttggcttgct tccagaggcg
     3661 atcatagagc agagaagctt tgtgggagcg aactcgtgca gaagccatcc atcgctcaac
     3721 attgctaaag cgcttctcta acgcggcccg cttcttggca acctcagagt tcacaatggg
     3781 cctcttgcca tagccgtgat tcgtatccag gccaagcggc acaagaaagt cgcgaatgat
     3841 attttcttgc accggccagc gccgtgtgta ggtctgggca agttccaccg catcgacggt
     3901 gtcagcggtg gtgacaatgg gaatcagctt ggcagtggtc cgagtcgcag gcgtcggctc
     3961 ggctttccac tccgcttgcc accaggcggg tttgcgaacg tcttcgtccg tttcttcttc
     4021 ttgccgcgta cagggaacgt gacggcgcag atcacgaatg agggccacgc gtaaaggaag
     4081 gaactggcct ttctggtcag gcagtgggag agcgaaacag gccggagcga cttcacgcac
     4141 cagtttcccg tgccgatccc gctcaagtgg cacaaacatc ccaacctcgg tgaaggattc
     4201 cagcccatca tattgatcag tgcgtagtac ggtcaacaca gtgtggccca gcgccttgag
     4261 atcgcgcagg aaaggagccg ccatgccctc ccgatccaca atcacgcgga cggatggtgc
     4321 ttcctcttcg cagtcttgat agcgcgccag aatcggtggc agccccatgg tcaagtgctg
     4381 gtctcctcga tgcgtcattg ccaatcgagg atgcccctgc tcatcgtgca agaacaccaa
     4441 cgctcgacac cccagaattt tgcccgagca tccaattaat ccgcgaggaa tcagtctgtc
     4501 tgcat
//
LOCUS       DASZAK010000691         1658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_160261, whole genome shotgun sequence.
ACCESSION   DASZAK010000691 DASZAK010000000
VERSION     DASZAK010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160261"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VGT44_12225"
     CDS             <1..561
                     /locus_tag="VGT44_12225"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2581610.1"
                     /translation="LGTEFDLPVDVVLVAIGEAPDPSFLPEGTSVGVAPWGGLLIHPE
                     SLSTGAPGVFAAGDVTYGPKSIIHAAAHGRKAARAIHAYLGHVALRSVSEMPEGEFET
                     ASTLPPDETITLDLRPTPREVMPLEQAEASHSRTIEFAKGFSEAQARREASRCLRCDL
                     AYLCPSIKVIGAALDESRLPVPVHPM"
     gene            637..1293
                     /locus_tag="VGT44_12230"
     CDS             637..1293
                     /locus_tag="VGT44_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581611.1"
                     /translation="MSVDQLTGAIVSGINSGAEQFLEGTLAAILPVLWLALLGLHLGR
                     PYILSMIDRFTLRLGADLLWLIYIALRDFLILSGVVMSFMFFFPDVVSTDQLPITGGL
                     AASALFAVLLIKLMGDPDNNLRDFRLVTYLLGLGALLYFIPYLFGVQFNNIGTTGTLA
                     NISTFLVSNTNPQWAVGIGYVSTALFAIMGAIAAGYTIITGGRAEVSEAAEIGETTTK
                     "
     gene            1311..>1658
                     /locus_tag="VGT44_12235"
     CDS             1311..>1658
                     /locus_tag="VGT44_12235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581612.1"
                     /translation="MKPNDVATAITQSLVLGGSSWLISTIVAFLPVLWTMALMLHLGR
                     PYILRTLRRCGLRLGADIWWMSYLLIRDAVLLITLGLSWFYFEPNIVYNAALPITGPL
                     ATLCLLLALAVKLT"
BASE COUNT          283 a          531 c          488 g          356 t
ORIGIN      
        1 ctggggaccg agttcgacct gcccgtcgat gtcgtactgg ttgccattgg cgaagcgccc
       61 gacccctcct tcttgccgga aggcacgagc gtgggagtgg cgccctgggg cggcctactc
      121 attcacccgg agtcgctttc gaccggcgcg ccgggcgtct tcgccgccgg tgatgtgacg
      181 tacggtccga aatccattat tcatgccgcc gcgcatggcc gcaaggcagc gcgcgccatc
      241 cacgcctatc ttggtcacgt cgcgctgcgc agcgtgagcg agatgcccga aggcgagttc
      301 gagacggcct cgacgctgcc gcccgatgag accatcacgc ttgacctgcg cccgacgccg
      361 cgcgaagtca tgccgctcga gcaggctgag gcgtcgcata gccgcacgat tgaattcgcc
      421 aaaggcttca gcgaggcgca ggcgcgccgt gaagccagtc gctgcctgcg ctgcgacctg
      481 gcttacctgt gtccatccat caaagtgatc ggagccgcgc ttgacgaatc gcggctacct
      541 gtaccggttc atcctatgta aaagaaatgc gtcgggacgc gcttgatcgc gccctgacgt
      601 attagagcca agaactctac taaaggaggt ttcctcatgt ccgttgatca acttaccggt
      661 gccatcgttt caggcatcaa ttccggggcg gaacaattcc tggaaggcac gctggccgct
      721 atcctgccgg tcctctggct ggcgctgctg ggcttgcacc tgggccgccc ctacatcctc
      781 tcaatgattg atcggttcac gctgcggctc ggcgcagact tgctgtggct catctatatc
      841 gccctgcgcg acttcctgat cttgtcgggc gtggtcatga gcttcatgtt cttcttcccc
      901 gacgtcgtct cgaccgacca gctgcccatc acgggagggc tggcagcatc cgcgttgttt
      961 gcggtactgc tgatcaaatt gatgggtgat cccgataata acctgcgcga tttccgcctg
     1021 gtgacctatc tgctgggcct gggcgcgctg ctctacttca tcccttacct gttcggggtg
     1081 cagttcaaca acatcgggac cactggcaca ctggccaata tcagcacctt cctggtgtcg
     1141 aataccaatc cgcagtgggc ggtcggtatc ggctatgtct caacggcgtt gttcgccatc
     1201 atgggcgcga ttgccgccgg ctatacgatc atcacgggcg ggcgcgcgga agtgtccgaa
     1261 gcagccgaaa tcggcgagac gaccacgaag tagaagaaag gaacaagact atgaaaccca
     1321 acgatgtcgc aaccgcgatc acccagtctc ttgtactggg cggttcgtcc tggctgatca
     1381 gcaccattgt ggccttcctg cccgttcttt ggacgatggc gctcatgctg cacctgggca
     1441 ggccgtatat cctgcgcacc ctgcgccgct gtgggctgcg cctgggcgcg gacatctggt
     1501 ggatgtccta cctgctcatt cgcgacgccg tgttgctcat cacgctcggc ctgagctggt
     1561 tctactttga gccaaacatc gtctacaatg ccgcgctgcc gatcaccggc ccgttggcca
     1621 cgctctgcct gctgctggcg ctggcagtca agctgacg
//
LOCUS       DASZAK010000692         1908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_444804, whole genome shotgun sequence.
ACCESSION   DASZAK010000692 DASZAK010000000
VERSION     DASZAK010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444804"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..1659
                     /locus_tag="VGT44_12240"
     CDS             376..1659
                     /locus_tag="VGT44_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581613.1"
                     /translation="MLKAKIDRKWFWRIVDFINPLRDPSLTVAAVLTFIEEAGQHDAW
                     YWIIVVALVNWVAVRGVVWLIVNFTFAGTFIMRRVWWAIRHPRQAWRLLDAVGHEEII
                     IGASALTEGMLLPDGSGMVSLSPVLTASGIQVIGELPAGTEIPLLSMPNGQMAPNLAA
                     LGSSGIFTGSASGALLGTNGFPGIQGIPGQAMPMGFPQLGPNGLISQQMTGGFPQQFP
                     QNFPQRPSEEEERLRQQLIAMSRLIDDWIQYTEEKWRTVSDSQLGWKPLRGRWGSWDD
                     IFQDAYAAKTSDPMAQAAHVAGEATTRRFEASREKGSAASAVACFMRDMLTLRQGMEI
                     AQPSLSGEPETPTFQPAVQPQYQPYQQYAQPSQQPQYPQYAQQPPSQQYTSQPAQQAQ
                     TGFGENTPPHHFPGVMPRNSLIRRPSPMYVDADNK"
     gene            1755..>1908
                     /gene="eno"
                     /locus_tag="VGT44_12245"
     CDS             1755..>1908
                     /gene="eno"
                     /locus_tag="VGT44_12245"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103948.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV2581614.1"
                     /translation="MTTIEDIVAREILDSRGNPTLEVEVMLTGGEHGLAAVPSGASTG
                     AHEAIEL"
BASE COUNT          417 a          531 c          561 g          399 t
ORIGIN      
        1 ggatgtcgac ttcaatgtgg taatgaccgc cgaacatgag tttattgaag tacagggaac
       61 cggcgaaggc ggcgtgttca gccgtcagac gatggaggcg ctggtctcac ttgcacaggc
      121 gggcatcgat gaactgctgg cgatgcagcg acgggtacta tagtaccgat ctgcaggaga
      181 atgcccttct acttcgtttt accattctag aggctttggt ctttgtaggg gcataccctt
      241 gcggtcgccc tggggatttt gccggtacag caggttcaga aatttgccga tacgcaaggt
      301 tcatggtaga atctcggcta tgactgccga aacaagatcg accggtcgga ccagcgacag
      361 ggtcgagagg agcaaatgtt gaaagcgaag atagaccgca aatggttctg gcgcatcgtt
      421 gattttatca atccgttgcg cgaccccagc ctgacggtcg ctgccgtgtt gacctttatc
      481 gaagaggcgg ggcagcatga tgcctggtac tggattatcg ttgtagcgct ggtgaactgg
      541 gtagccgtgc gcggtgtggt ctggttgatc gtgaacttca ccttcgccgg cacatttatc
      601 atgcgacgcg tctggtgggc tatacggcat ccccgccagg catggcgatt gctcgacgcg
      661 gttggtcacg aagagatcat tatcggcgca tcggcgctta ccgaagggat gctccttccg
      721 gacggttccg gtatggtcag cttgagtccg gtgctgaccg cgagcggcat tcaggtcatc
      781 ggcgaactgc ccgccggaac cgagattccc ctgttgagta tgccaaatgg gcagatggcg
      841 ccaaacctcg ccgcgctcgg cagttctggc atatttaccg gctctgcctc tggtgcgctc
      901 ctcggcacga atggttttcc tggcattcag ggtatacctg gacaggcaat gccgatgggt
      961 ttcccgcagc ttggtcccaa cggccttatc agccagcaaa tgaccggtgg cttcccgcag
     1021 caatttccgc aaaacttccc gcagcgtccc tctgaagaag aagaacgcct gcgccagcaa
     1081 ctgatcgcca tgtcacgctt gatagacgat tggattcaat ataccgagga gaaatggcgc
     1141 accgtcagtg acagccagct tggctggaaa cccctgcgcg gcagatgggg cagttgggac
     1201 gatatcttcc aggatgccta tgccgcgaag acttccgacc caatggcaca ggcggcccac
     1261 gtggccggag aggccaccac gcgacgcttc gaggcatcac gcgagaaggg atcggcagcc
     1321 tcggcggtgg cttgcttcat gcgcgacatg ctgacgctgc gccagggtat ggagatcgcg
     1381 cagccgtcgc tgagcggcga gccggaaacg ccaacatttc agccagccgt tcagccgcag
     1441 taccagccat atcaacaata tgcacagccg tcgcaacagc cacaatatcc gcaatatgca
     1501 cagcagcctc cgtcccagca gtacacgtca cagcctgcac agcaagcaca gaccggtttc
     1561 ggtgaaaata ctccacccca ccacttccca ggagtgatgc cgcgcaattc cctgatccgc
     1621 cgtccatcac ccatgtacgt cgacgcggat aacaaataaa aggtaaactt gctcgtaatc
     1681 aatctccggt gtacaatacg tcgcgagagg tattgtaaca acggaggttt tttatgcctt
     1741 tttcgatgag cgatatgaca accatcgagg atattgtggc gcgcgagata cttgactcgc
     1801 gcggcaatcc gacgctggaa gtggaagtga tgttgaccgg aggcgaacat ggcctggctg
     1861 ctgtcccttc aggagcttcg acgggtgcgc acgaggcgat cgagctgc
//
LOCUS       DASZAK010000693         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_692837, whole genome shotgun sequence.
ACCESSION   DASZAK010000693 DASZAK010000000
VERSION     DASZAK010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692837"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..493)
                     /locus_tag="VGT44_12250"
     CDS             complement(14..493)
                     /locus_tag="VGT44_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2581615.1"
                     /translation="MGFLVAFLVIFWISLIIWTFRDIRSRTQDWLAQILATLLVFVFF
                     IGGLFIYMILRPRQTLAEIYERQLEEESLLAEMTERQTCSNCHARIEGDFQICPSCGQ
                     KLKRPCPKCERLLELRWTFCPYCATNLAPIPVQSIPGMPIMQGGPGVPMSGQLGPRY"
     gene            complement(898..>2337)
                     /locus_tag="VGT44_12255"
     CDS             complement(898..>2337)
                     /locus_tag="VGT44_12255"
                     /inference="COORDINATES: protein motif:HMM:NF019368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEV2581616.1"
                     /translation="VLVDLALTPPGQVKLHHASLYAAERHLLALLSREDIHTFLITVT
                     DLARATIEFVAADLPAYGLKTFWLYPHGLPTEDVAEQQFIASSPPSASSTPNAIENQY
                     YCVEANPSDGTLTVTEKQSGLVYPGLNRFIDGGDIGDLYNYCPPARDLLISAPAEPPV
                     IEAHHSPARSQLRIHAILSLPAACSSDRASRGDERVACPITSEVTLAPGMRRIDIHTS
                     VDNCARDHILRVAFPVSYVVETASAEGVFEVRERPAAQPLPPDVAGWIEQPVNRFPQK
                     RFVEVSDGTHALAVLNRGLPEYEIIADEHGQSAIAVTLLRCIEWLSRGDLTTRRGHAG
                     PALHTPEAQCQGQSVFDYALVLHGGDWEAEDALALREAQAFNTPVLVVISDQHTGELP
                     SQASLVEVEPRSLVVSAIKRPHDGGLVVRVYNPLRRAVEAAIRPGFAATRVFTTNLVE
                     EPQEQLLWSGDEPLYVGLRAGEIATLHFS"
BASE COUNT          455 a          631 c          787 g          464 t
ORIGIN      
        1 taccgccctg tggtcaatag cgggggccta gttggccgct catggggacg ccagggccac
       61 cctgcatgat gggcatccct gggatgctct ggacggggat gggcgcgagg ttggtcgcgc
      121 agtaggggca gaaggtccag cgcagttcga gcaggcgttc gcatttgggg catggacgtt
      181 tgagcttctg gccacaggat gggcagatct ggaagtcgcc ttcgatgcgg gcgtggcaat
      241 tgctgcaagt ctgccgctcg gtcatttcgg ccagcaggga ctcttcttca agctggcgtt
      301 cgtagatttc ggccagcgtc tggcgcggtc gaaggatcat atagatgaag aggccgccaa
      361 tgaaaaaaac gaatacgagc agggttgcta atatctgtgc aagccagtcc tgggtgcgcg
      421 aacgaatgtc gcggaatgtc cagatgatca ggctgatcca gaaaataacc agaaacgcta
      481 caagaaaacc cacggctact cctaacactt tgttcaaggc atctaataca ctgtttcccg
      541 tggtaggggt tgcagcaatc acgaatatat cattcacact cacaggtcta cgcccctttc
      601 agcatacgca aggctataca tgtaatacaa cgtaaccgat gcgcaaaaag gttacaatcc
      661 aggacgatgt agtaaatatt ccctacctac tttatccaag tgcgctgagg cgcgattcgt
      721 cgcgcccgtc ggatgtaggt gccggttcac cgcgcacacc gccgattgat cggccaccac
      781 cggcgtatcg agcaagccat caggccgatc aatcggcggt gtgcgcggtg aaccggcacc
      841 tacggtttat atgcgcattt ttacccgttt attttgttaa tcagcatcat cgcgtcgtta
      901 cgagaagtgc aaggtggcga tctccccagc gcgcaggccg acgtagagcg gttcatcgcc
      961 actccacaag agttgctcct gcggctcttc gaccaggttg gtcgtgaaga cgcgagtggc
     1021 ggcgaagcct ggccggatgg ctgcttcgac ggcgcggcgc aatggattat agacgcgcac
     1081 aacaaggccg ccgtcgtgtg ggcgtttgat ggcgctcaca acgaggctgc gcggctcaac
     1141 ctcgaccagg ctggcctgcg atggcagctc gccggtgtgc tggtcgctga tgacgacgag
     1201 tacgggcgta ttgaatgcct gcgcttcacg cagcgccagg gcgtcttcgg cctcccaatc
     1261 gccgccgtgc aggacgaggg cgtagtcgaa gacgctctgc ccctggcatt gcgcctccgg
     1321 cgtgtgtagg gccggtccgg cgtggcctcg acgggtggtg aggtcgccgc gcgagagcca
     1381 ttcgatgcag cgcagcagcg tgacggcgat ggcgctttgc ccatgttcat ccgcgatgat
     1441 ttcgtattct ggcaggcccc ggttcagcac agccagggcg tgtgtaccgt cgctcacctc
     1501 cacaaaacgc ttttgcggga agcgattgac cggctgctct atccacccgg ccacgtctgg
     1561 cgggagaggt tgagctgccg ggcgctcgcg cacctcgaag accccctcgg ctgaagcggt
     1621 ctctactaca tatgatactg gaaacgcaac acgcaggata tgatctctgg cgcaattatc
     1681 cacgctggta tgaatgtcga tgcggcgcat gcctggggcg agcgtgacct cgctggtaat
     1741 ggggcaagct acgcgctcgt cgccccggct ggcgcggtcg gacgagcagg cggctggcag
     1801 cgacaggatg gcgtggatgc gcagttgcga gcgcgcgggg gagtgatgcg cctctatcac
     1861 gggcggctcg gcgggcgcgc tgatgagcag gtcgcgggcg ggcgggcaat agttgtaaag
     1921 gtcgccgata tcgccgccat cgatgaaacg gttcaggcca gggtagacca ggccggattg
     1981 tttctcggtg acggtcaggg tgccatcgga tgggttggct tcgacgcagt agtattgatt
     2041 ctcgatagcg ttgggcgtac ttgacgcgga aggcggcgag gaggcgatga attgttgctc
     2101 cgcgacatcc tctgtgggca ggccgtgtgg gtagagccag aaggtcttga ggccgtaggc
     2161 gggcaggtcg gcggcgacaa attcgattgt ggcacgcgcc aggtcggtga cggtgatgag
     2221 gaaggtgtgg atgtcttcac gcgataggag ggcgagcagg tgacgttcgg cggcgtagag
     2281 cgatgcgtga tggagcttca cctggcctgg aggggtcagg gcgagatcga cgaggac
//
LOCUS       DASZAK010000694         3237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_160311, whole genome shotgun sequence.
ACCESSION   DASZAK010000694 DASZAK010000000
VERSION     DASZAK010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160311"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1646)
                     /locus_tag="VGT44_12260"
     CDS             complement(<1..1646)
                     /locus_tag="VGT44_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEV2581617.1"
                     /translation="MTELNQKKQDEYLFGWDDMPGIVSVWASRDGRALVWRRDGEQVT
                     FAHERFRPWLFAASLDDLAHTNATPVDATTVQPGITYQELEGPLDSYHFLLAANDGRA
                     LEREIVRGASRRLNRQVSSINELQNEYYRVGPVEQYLMQSGRVYFRSLSYDEVYRLQF
                     DLETTALDPHRGRIFMVAVRDSQGLVLTLDAPRPEDEARLIADLCALIRERDPDIIEN
                     HNLFGFDLPFLEQRAAVNRIPLRIGREGGPVLLERRDETLAIGPEARRRVRYSLAGRE
                     LIDTLDAVRRYDFVVRDMPSYGLKDVARYFGIASSERVYIEGAAIFETYRRDPQRVRQ
                     YALDDVAEVDGLSRRLLGAPFALASMAPRRYERLASAGPAMGILEPILVRAYLRAGAA
                     LPRRSTLQGAESIMHEGGAVHLFAEGIAEHVVKADVASLYPSLMRTFQIGPTSDRLGV
                     LLAILGRLTDLRFAHKAAAKAAAPGSIEANQHEATQAAMKILVNSAYGYMGAGSMALF
                     ADSRAADEVTRRGRETLDQVLDALRQRKIALIEADTDGVY"
     gene            1913..2836
                     /locus_tag="VGT44_12265"
     CDS             1913..2836
                     /locus_tag="VGT44_12265"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV2581618.1"
                     /translation="MKPPRFQYCAPRMLDEALYLLDAYAGDVKVLAGGQSLVPLLNMR
                     LAGPAYLVDINHIAELHYIETEQDGLTIGALARQRQVERSILVQDRHPLLARTIEHIG
                     HMQIRNRGTIVGSIAHADPAAELPALLTCLDGEVLAQSIRSERLIKAQDFFTGYLSTA
                     LEPNEMLVEARFPWISEQAGWSFMEFARRSGDYALVGVAAVLTPAADDTCQSASIAYL
                     GVAGAPVRGHAIEALLTGSRLDEQTLDAAAEAARDVVSDDMNDVHATVEYRRSLAAEM
                     TRRALTDAWGRRGRQAQPDQSNIWNEQNGEA"
     gene            2836..>3237
                     /locus_tag="VGT44_12270"
     CDS             2836..>3237
                     /locus_tag="VGT44_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2581619.1"
                     /translation="MRERDDDVSQFAEQCEIAVTVNGHRHERSVPVRLLLSDFLRHDL
                     GLTGTHVGCEHGVCGCCTILFNGEAVRSCLMFAVQADGIAITTIEGMAGSQSDLHPIQ
                     QAFMEKHGLQCGFCTPGIIMSVHALLHEHPNP"
BASE COUNT          669 a          974 c          952 g          642 t
ORIGIN      
        1 aagtaaacgc catcggtatc cgcttcgatc aatgctattt ttcgttgtcg cagagcatcc
       61 agcacctgat ctagcgtttc acgccctcgc cgtgtcacct cgtctgcggc gcggctatcg
      121 gcgaagagcg ccattgaacc ggcgcccatg tagccatagg ccgagttgac caggattttc
      181 atagccgcct gtgtcgcctc atgctggttt gcctcgatgg agccaggcgc agcggctttc
      241 gcagcggcct tgtgcgcaaa gcgtagatcg gtcaagcgtc ccaggattgc gagcagcacg
      301 cccaggcggt cgctggtcgg gccgatctga aacgtgcgca tcagcgacgg atacagcgaa
      361 gcaacatcag ccttcaccac atgctcggcg atgccctctg cgaaaagatg cactgcgccg
      421 ccctcgtgca tgatgctttc agcgccctgt agggtagatc ggcgtgggag cgccgcgccc
      481 gcgcgcaggt aggcgcgtac cagaattggc tccagtatgc ccatagccgg gccagccgag
      541 gccagccgct cgtagcgacg aggcgccatg cttgccaggg cgaagggcgc gccgagcagc
      601 cgacgcgaca acccatcgac ttccgctaca tcatccaggg catactggcg cacgcgctgc
      661 gggtctcgtc ggtaagtctc gaagatggcg gcgccctcga tatagacccg ctccgagctc
      721 gcgatgccga agtagcgcgc gacatccttc aggccataac tcggcatatc acgtaccacg
      781 aaatcatagc ggcgcaccgc gtccagcgtg tcgatcaact cgcgtccggc aaggctgtag
      841 cgcacacggc ggcgcgcctc tggcccgatg gccagcgtct cgtcgcgtcg ctccagcagc
      901 accggtccgc cttctctacc aatgcgcaag gggatgcgat tgactgccgc gcgctgctcc
      961 aggaacggga ggtcaaatcc gaacaggtta tgattttcga tgatatccgg gtcgcgttct
     1021 cgaatgagcg cgcagaggtc cgcgatgaga cgcgcttcat cttccggacg cggcgcatca
     1081 agtgtgagga ctaatccctg actgtcgcgc actgcgacca tgaagatacg cccgcgatgc
     1141 ggatcgagcg cggtcgtctc aagatcgaat tgcaggcgat acacctcgtc atacgacagg
     1201 ctacgaaagt agacacgccc gctctgcata aggtattgct cgaccggacc aacgcgatag
     1261 tactcgttct gcaattcatt gatgctggat acctgccgat tgagtcgccg agaggctccg
     1321 cgtactattt cgcgctcgag cgcgcgtcca tcgttggcgg caagcagaaa gtggtaggaa
     1381 tcaagagggc cttcaagttc ctggtaggtg atgccgggtt ggacagtcgt tgcatcaact
     1441 ggcgtcgcat ttgtatgggc gagatcatcc aggctggcgg caaacagcca ggggcgaaag
     1501 cgctcatgtg cgaaggtgac ctgttcacca tcgcgccgcc acacaagcgc gcgaccatcg
     1561 cggctggccc acacagacac gatccccggc atatcgtccc agccgaagag atattcgtct
     1621 tgctttttct ggttcaattc agtcatgttc aatcaatcat atcatacgaa agaggtcact
     1681 ttcattccgc tttaaattcc gatttgctgc cggagtttgg tacacaccgt gctggtcagt
     1741 acggctcata gactctgacg gaaaaaagct gttaaatagg cttgacagga gcgcgagaca
     1801 ggagtacttt aatttctatt ctcgcgcgaa caagcgcagc gacagttaaa gatgattccg
     1861 cctgtatttc cgcagctgtt cgcataacac atactatttg agggggctgg caatgaaacc
     1921 tccacgtttc cagtattgcg cgcctcgcat gttggacgag gcgctctatc tgctcgatgc
     1981 gtatgccggt gacgtaaagg tcctggcggg cgggcagagt ctggtgccgc tgctcaatat
     2041 gcggctggcc ggacccgcct atcttgtcga tatcaaccat atcgccgaac tgcattacat
     2101 cgaaaccgaa caggatggcc tgaccatcgg cgcgctggcc cgccagcgcc aggtagaacg
     2161 ctccattctc gtgcaggata gacatccgtt gctggccagg accatcgagc atatcggcca
     2221 catgcaaatc cgcaatcgcg gcaccatcgt cggcagcatc gcccacgccg accccgccgc
     2281 cgaactcccc gcgctgctga cctgcctgga tggtgaggtg ctggcgcaga gcattcgcag
     2341 cgaacgcctc atcaaagcgc aagatttctt taccggctac ctctcaacag ccctcgaacc
     2401 gaacgagatg ctggtcgaag cgcgctttcc ctggatatca gagcaggccg ggtggtcatt
     2461 catggagttc gcgcgccgct ctggagacta tgccctggtc ggtgtagctg cggtgttgac
     2521 tcctgcagcc gatgatacct gtcagtcagc cagcattgcc tatctcggcg tggcaggcgc
     2581 tcctgtgcgc ggacatgcca tcgaagcgct gctcaccggc tccaggctgg atgagcagac
     2641 cctggacgcg gcagctgagg cggcgcgcga cgttgtaagc gacgatatga acgatgtcca
     2701 tgccactgtc gaataccggc gctcgctggc ggcggaaatg acccggagag ccctgacgga
     2761 tgcctgggga agacgcggtc ggcaggcgca gccagatcag tcgaatatat ggaacgagca
     2821 gaatggagag gcataatgcg agagcgcgat gatgatgttt cgcaatttgc agagcaatgt
     2881 gagatcgcgg tcacggtcaa tggacatcgc cacgaacgca gcgtccctgt acgcctgctg
     2941 ctctccgatt tcttgcgcca tgacctgggc ctgacgggaa cgcatgtggg ctgcgagcat
     3001 ggcgtctgcg gctgctgcac cattcttttc aacggcgagg cggtgcgttc ctgcttgatg
     3061 ttcgctgtgc aggccgatgg catagccatc accaccatag aaggtatggc gggaagtcag
     3121 agcgacctgc accccatcca gcaagccttt atggagaagc atggcctgca atgtggattc
     3181 tgcactcccg gcatcatcat gtcggttcac gcgctgctgc acgaacaccc aaatccg
//
LOCUS       DASZAK010000695         4423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_123693, whole genome shotgun sequence.
ACCESSION   DASZAK010000695 DASZAK010000000
VERSION     DASZAK010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123693"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..2036)
                     /gene="ftsH"
                     /locus_tag="VGT44_12275"
     CDS             complement(39..2036)
                     /gene="ftsH"
                     /locus_tag="VGT44_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV2581620.1"
                     /translation="MDAQAHFLYQRNPNERPNPQQRPDLNRGQNSNGNNGGPPRNSSS
                     SLVVRILILVGIVLVGWYLIQLFIPSGSSSNSNAVEIPYSTFYQEVQAGNVKDVTFSG
                     QDANGDFTSTQTITDVTGVSKQGTTFHFTQLPNGDPNLTTLLQKYHVNYSAKPASDNS
                     FLFTILTTVVPWIFIIGFFFFIARRATQGQQNIFSFGKSRAKVVLEDRPSTTFADVAG
                     VDEAKNDLVEVVEFLKTPGKFQKLGGRIPRGVLLVGPPGTGKTLLARAVAGEAGVPFF
                     SMSGSEFVEVLVGVGASRVRDLFDQAKKASPSIIFIDEVDAVGRQRGSSINSNDEREQ
                     TLNQLLVEMDGFDTRQAVVVIAATNRPDGLDKALLRPGRFDRRVSVERPDWNGRLAIL
                     KIHSRDVPMGPDVDLTAVARSTPGMVGADLANLVNEAALLAARRNLDAVNQRCFEEAL
                     DKIVIGAERPLVLSEDDLNVVAYHEGGHALTGLLTENVDPVTKVTIVPRGQALGVTQY
                     TPLDDRYNYSKEYLESQLVTALGGRAAEEVAIGHITTGAENDLQRVTAIARQMVTRWG
                     MSERLGTISYSEREDPFAGTALATGSREYSEKTATVIDEEVNGIVKWAYNRSLELLTR
                     HRATLDRIARDLRLYETLDARQLRSILEETGSIDAAPASYR"
     gene            2319..4019
                     /locus_tag="VGT44_12280"
     CDS             2319..4019
                     /locus_tag="VGT44_12280"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581621.1"
                     /translation="MEHRYALLVGIDHYNDAQHFIPHSLAQADAQSLYELLIDPERGG
                     WLPENVVYLAGATASRDEIESQLREVCLVRAQPGDLVLLYFAGYALLDPATQDGYLAL
                     SATQAERPATGLHVPTLVDHYLYDSKASHILTVLDIVHAGVIWKHDKPVDNAFMLFGQ
                     SLADLPRDRGRVALVSHRPAEVSRSQMESGRGAFMARLIDGLEGEAANPHTGRITLGT
                     LYDYLDETIGRDEIQYPQKFGHEYGSMNLIEWAEWRTASAPSTPVKDRRVAAVEITPL
                     HILTGHRGHVDDVVFSPDGAKVASCGEDMTVRLWSTGAGELLHTITGHAGAVMGVDYA
                     PDGKVIASCSEDKTVRLWDAQTGEALQVLQGHNSAVWTVAFSIDNQMLASCSNDETVR
                     IWNPVTGELLHTLEGHHNVVVGVDFSYDSTLLASCSFDKTICLWDARSGELQRRLRYS
                     DIVYGVAWSPDGNLLASCSADGTICLWDTSNGQLTQVLTGHDGAVWTVDFSADGRLLV
                     SGSEDGSLKLWDVSQGRELANINHRIEVYGVVFGANGLLANSAEDGAVRIWQTEVIEG
                     "
     gene            complement(4073..>4423)
                     /locus_tag="VGT44_12285"
     CDS             complement(4073..>4423)
                     /locus_tag="VGT44_12285"
                     /inference="COORDINATES: protein motif:HMM:NF028447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5110 domain-containing protein"
                     /protein_id="HEV2581622.1"
                     /translation="PDPLTFVLYPCEGSGIATFYEDAGDGFEYSNDVYARRTITCAVE
                     AGHIRIVIGEQEGTFVSTRQDIRLELREVASEPEAVQIGETSATWHYHSEQRRLTIDL
                     DTIAFSQLVELSIE"
BASE COUNT          863 a         1249 c         1368 g          943 t
ORIGIN      
        1 cgccccggcg accaggccgc tcgtttgtct ccatctactt acctgtacga ggcgggcgcg
       61 gcgtcgattg accccgtctc ttcgaggatg gagcgcagtt gacgagcgtc gagcgtctcg
      121 tacaggcgca ggtccctggc gatccggtcg agcgtggcgc gatgcctagt caggagttca
      181 agcgagcgat tataagccca cttgacgatg ccgttgacct cttcgtcgat aacggtggcc
      241 gtcttctcgc tatattcgcg agagccggtc gccagggcag tgccggcaaa ggggtcttcg
      301 cgttcgctgt aggagatggt tcccaggcgc tcgctcatgc cccagcgcgt gaccatctgg
      361 cgcgcgatgg cggtgacgcg ctgcagatcg ttctccgcgc cggtggtgat gtggccgatg
      421 gccacctcct cggcggcgcg cccacccagg gccgtgacca gttgcgactc caggtattct
      481 ttcgagtagt tgtagcggtc gtcaagcggt gtgtactgcg tgacgccaag cgcctggccg
      541 cgcgggacaa ttgtcacttt ggtgacggga tcgacgtttt cggtcagcag gccggtcaga
      601 gcgtgaccgc cttcgtgata ggcaaccacg ttgaggtcat cctcgctcaa caccagcggg
      661 cgttccgcgc cgattacaat cttgtcgagg gcttcctcga agcagcgctg gttgacggca
      721 tccaggttgc ggcgcgccgc cagcagcgcc gcctcgttga ccaggttggc cagatccgcg
      781 cccaccatgc cgggggtact gcgcgcaaca gccgtgagat cgacatctgg ccccatgggc
      841 acgtcgcggg agtgaatctt gaggatggcc agccgcccgt tccagtcggg tcgttcgacg
      901 ctgacacggc gatcgaagcg gccaggacgc agcagggctt tgtccagtcc gtcgggtcta
      961 ttcgtagcgg cgatgaccac caccgcctgg cgtgtatcaa agccgtccat ctcgaccagc
     1021 aactggttga gggtttgctc gcgttcgtcg ttgctgttga tgctggaacc tcgctgacgg
     1081 cctaccgcat cgacttcatc gatgaagatg atgctgggcg aggctttctt cgcttgatcg
     1141 aagaggtcgc gcacccggct cgcgccgacg ccgaccagca cttcgacgaa ctcggaacca
     1201 ctcatggaga agaacgggac gccggcttct cctgccacgg cgcgcgccag cagagtcttg
     1261 ccggttcccg gagggccgac cagcagcaca ccgcgcggaa ttctcccgcc caacttctgg
     1321 aacttgccgg gcgttttgag gaactccaca acctcgacaa gatcattttt ggcctcgtcc
     1381 acgccggcca catccgcgaa ggtggtgctt gggcgatctt ccaataccac ctttgcgcgg
     1441 ctcttgccaa agctgaaaat gttctgctgg ccctgcgtgg cgcggcgcgc gatgaagaaa
     1501 aagaagccaa tgataaaaat ccagggaacc acggtggtga gaatggtaaa caaaaagcta
     1561 ttgtccgacg cgggtttcgc cgaatagttg acgtggtact tttgcagcag agtggtcagg
     1621 ttcggatcac cattcggcaa ctgcgtgaag tggaaagtcg ttccctgctt ggaaacgccg
     1681 gtcacatcag tgatggtctg tgttgaagta aaatcgccat tggcatcctg ccctgagaac
     1741 gtgacatcct ttacgtttcc cgcttgaact tcttgataga aggtgctgta tggtatctca
     1801 acggcgttgg agttgctgct actaccgctg ggtatgaaga gctggatgag ataccagccc
     1861 accaggacta tgccgaccag aatcagtatg cgcaccacaa gcgagctgct agagttacga
     1921 ggcgggccgc cgttattgcc gttgctgttc tgccctcgat tgaggtcagg acgctgctga
     1981 ggatttggcc tctcgtttgg attgcgctgg tataaaaaat gcgcctgagc gtccatgtct
     2041 tccgacctgc tttctgaatg tgatcggcgc gtcaaccgcg ccgatcacag gaattgacaa
     2101 ttgtattata cgatatatcc catcgtccct acgtaccatt tctgatgatt acgtaatcta
     2161 tacgtaatag attaccaaaa gttgagcaat ttagcaatct ttgaaagcga gtgccagggt
     2221 aagccagggc gaccgcgagg gtcgccctgg cttactggcc aatattgtat aatctctatc
     2281 gtgtgccaac taagagtaat acgaaaatga ggtacgacgt ggaacaccgg tatgcactac
     2341 ttgtaggtat agaccattac aacgatgcgc agcattttat tccccattcg ctggcgcagg
     2401 cggacgcgca gagcctgtac gaactgctga tcgacccgga acgcggcggc tggctgccgg
     2461 aaaacgtggt gtacctggct ggggcgactg cgtcgcgcga tgagatcgag agccagttgc
     2521 gtgaggtgtg cctggtgcgc gcgcagccgg gcgacctggt cttgctgtat tttgccgggt
     2581 acgccctgct tgaccctgcc acccaggatg gctacctggc attgagcgcg acgcaggcgg
     2641 agcggcccgc gacggggctg cacgtgccaa cgctggtcga tcactatctc tacgactcaa
     2701 aagcttcaca tatcttgacg gtactggata tcgtacacgc cggagttatc tggaaacatg
     2761 acaagccggt ggataacgcc ttcatgcttt tcggccagtc gcttgccgat ttgccgcgtg
     2821 accgggggcg cgtggcgctg gtgtcacacc ggcctgcgga agtatcgcgt tcgcagatgg
     2881 aatccggtcg aggcgcgttt atggcgcgcc tgattgatgg gttggagggc gaggctgcca
     2941 acccgcacac cgggcgcatt accctgggga cgctttatga ctatctcgat gaaacgattg
     3001 gtcgggatga aatacagtat ccacaaaagt ttggccatga atacggctca atgaacctga
     3061 tcgaatgggc ggagtggaga actgcttccg caccatcgac gccggtgaag gaccgccgcg
     3121 tggctgctgt ggagattacg ccgctgcaca tcctgaccgg gcatcgcggc catgtcgatg
     3181 acgtggtgtt ctcgcccgac ggcgcgaaag tggcgtcgtg tggtgaagac atgaccgtgc
     3241 gcctgtggtc taccggggcg ggtgagctgc tgcataccat caccggccac gccggcgcgg
     3301 taatgggcgt ggactatgcc ccggatggca aggtaatagc atcctgctcc gaggataaga
     3361 ccgtgcgcct gtgggatgcg cagacgggcg aggcgctaca ggttttacag gggcataaca
     3421 gcgcggtctg gacggtagct ttctcgattg ataaccagat gctggcctct tgctcgaacg
     3481 acgagacggt gcgtatctgg aatcccgtca caggcgagct attgcatacg ctggaagggc
     3541 atcataacgt ggtggtcggc gttgatttct cctacgatag tacgctgctg gcctcgtgtt
     3601 cgttcgacaa aaccatctgc ctgtgggacg cgcgcagcgg ggagttacag cgccgcctgc
     3661 gctacagcga tatcgtctac ggtgtagcgt ggtcgcccga cggcaacctg ctggcctcgt
     3721 gttcagccga tggcacgatt tgcctgtggg atacttccaa cggccagctc acgcaggtgc
     3781 tgaccggaca cgatggcgcg gtctggacgg tcgatttctc cgccgatggc cgcctgctgg
     3841 tttccggctc cgaggacggc tcgctgaagc tctgggatgt ttcgcagggc cgcgaactgg
     3901 ctaacatcaa ccatcgcatc gaggtctacg gcgtcgtctt cggcgcaaac ggtctgctgg
     3961 caaatagcgc ggaggacggg gcggtacgca tctggcagac cgaggtaatc gaaggatgag
     4021 gaggggtgtc cttgcggtcg ccctggtaat gagaattcca gcgtatctcc aattactcta
     4081 tcgacagctc aaccagctgc gaaaaggcta ttgtatccag gtcgatggta aggcgcctct
     4141 gctcggaatg atagtgccag gtagcagaag tttccccgat ctgaacggcc tctggctcgg
     4201 aagcaacttc gcgcagttcc agccgtatat cctggcgcgt ggagacaaac gtgccttcct
     4261 gctcgcctat tactatgcga atatgtccgg cctcgaccgc gcaggtgata gtgcgccgcg
     4321 catacacgtc gttggagtac tcaaagccat cgccggcgtc ctcgtaaaac gtggctatgc
     4381 cacttccttc gcagggatag agtacgaatg tcagcgggtc agg
//
LOCUS       DASZAK010000696         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_267188, whole genome shotgun sequence.
ACCESSION   DASZAK010000696 DASZAK010000000
VERSION     DASZAK010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267188"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VGT44_12290"
     CDS             <1..1306
                     /locus_tag="VGT44_12290"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2581623.1"
                     /translation="ERMQEVLDQAPEITESATPYGGPERLRGEITFQNVVFGYTPERP
                     ILKGINLQITAGKKVALVGLSGGGKTTLIKLIPRFYEVNQGSVKIDSIDNRNIPLAIL
                     RRNVSQVLQESVLFEGTILENIKLGRPEATDEEIVAAAKQAQIHDTIMGLPEGYETKV
                     RERGKNFSGGQRQRLAIARAILTNSPILVLDEPTASLDVEAEVEVLRAIDKLVVGRTV
                     LMISHRLSTLGNVDEIIVLKDGRIAEQGTYQDLKRSNGVFAGLLKEQNRYNVDYNGGS
                     MIVPKAELERLINQQGRGAAPYQQPSPIAPPPVRPGQVPVPPGRDRYMMGGSGNGNQQ
                     LLNARVLIEVDGRIVGQRQLDKPVLTVGRLSSNDVPVPSQRVSRLHAKIRWVKEYGAW
                     VIEDAESLNGLILNGHRIDQHTLRKGDRIYVSPSAVLQFEPL"
     gene            complement(1322..2968)
                     /locus_tag="VGT44_12295"
     CDS             complement(1322..2968)
                     /locus_tag="VGT44_12295"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HEV2581624.1"
                     /translation="MGMIHGGWLVAKFLKREGVEVVFTLSGGHIAAIYDGCKREGIRV
                     VDTRHEQAAVHAAEGWAKCTRTPGVALLTAGPGVTDGVTGVANAYLAGSPILVIGGAA
                     PLSLWDRGALQEMNQIDLLRPITKWARTVHETARLGEYTASAFRQMLNGKPGPTFLEM
                     PMDVLNNLADTDTLFDPGEPASYRYGGRTAPDPAMVVKAAALLEKAQRPVIMAGTAVW
                     WCDAAEPLRQLAERINAPVFLNGAGRGCLPPTHPLFFTSSRRKALEGADTILAIGTRM
                     DFRLNHGQPPLIPKEANVIWFDLAGEDVGVNRGAAAGLVGDVGLSMQQLSEATRQLDE
                     GNWLAYIRREERNALGRDGALMSSDDVPIHPMRFCREIRDFIDEDTTVVGDGGDIVSY
                     GGRVINVDKPGYWLDPGPMGCLGTGTGFAMAAQIARPGKKVLILHGDGAFGLNGMEFE
                     SMVRQQLPVVSIIGNDGAWGQIKHPQKAILGHATAAELSPGIRYDKMVEALGGYGELV
                     QRPEDIRPALERAFASGLPACINVLIDPNKPYSRSTNVAV"
     gene            3202..>3943
                     /locus_tag="VGT44_12300"
     CDS             3202..>3943
                     /locus_tag="VGT44_12300"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEV2581625.1"
                     /translation="MPFRITHLHSADKLCRTLTWPILEHLYPRQRIEHLVETFCGQTT
                     RVRKLSGVLTVWVLICWHLYLRHSLGAVFLKLSSAERWLAEDEPESPPTRAAWTYRRK
                     QLGVRLLRALFEESCVPLATQDTPGAFAFGLRLMAIDGTLEDVNDTPANAQYFGRISD
                     GQTSSPYPQLRCIYLLEAGTHAIVKVIAAPCKASEQCLARGLLPAIKPGMLVLVDRGF
                     VSGALLEAICARGAQVLGRLPQGIFMHKE"
BASE COUNT          855 a         1136 c         1123 g          829 t
ORIGIN      
        1 cgagcgcatg caggaagtac tggaccaggc tcctgaaatc actgagagcg caacgcctta
       61 cggcggccca gagcggctga gaggcgagat cacctttcaa aacgtggtct ttggctacac
      121 gccggaacgc ccgatactta agggcatcaa cctgcaaata accgcaggca aaaaagtcgc
      181 gctcgtcggc ctgtccggag gcggcaaaac aacgctgatt aagttgatcc ctcgctttta
      241 cgaagtcaac cagggaagcg ttaaaatcga cagcatcgat aaccgtaata ttccactcgc
      301 tatattacgc cgcaatgtca gccaggtatt gcaggaatcg gtgctgttcg agggaaccat
      361 cctggagaat atcaagttag gacgaccaga agctacggat gaggagattg tagcggcagc
      421 aaaacaagcg caaatccacg atacaatcat gggtttgccc gaaggctacg aaacaaaggt
      481 acgcgaacga gggaagaatt tctcaggcgg acagaggcaa cgcttagcaa tcgcacgtgc
      541 tattctgacg aattcgccca tcctggtgct ggacgagcct accgccagcc ttgatgtaga
      601 agcagaggtc gaggtgctgc gagcaatcga caagcttgta gttggacgca ccgtcttgat
      661 gatttctcac cgcctgagca cgctgggcaa tgtggatgaa atcattgtgc ttaaagacgg
      721 acgtattgcc gagcaaggta cttaccagga tttgaagcgc agcaacggcg tgttcgcggg
      781 tctgctcaaa gaacagaatc gctacaacgt tgactacaat ggtggatcga tgatcgtgcc
      841 aaaggctgag cttgagcgct taataaacca gcagggccgg ggtgcagcgc cataccaaca
      901 accctctcca atcgcaccac ctcccgttcg cccaggacag gtgcctgtac caccgggtag
      961 agaccgctat atgatggggg gaagtggcaa tggaaaccag caactgctca acgctcgcgt
     1021 attgatcgaa gtggatggaa ggattgtggg acaacgacag cttgataagc ccgtgcttac
     1081 cgtagggcgc ctctcaagca acgatgtgcc ggtgccatct caacgtgtat cgcgcctgca
     1141 cgcaaagata cgctgggtaa aggaatacgg ggcctgggtg attgaggatg ccgagagcct
     1201 gaacggattg attttaaatg gacatcgtat tgatcaacac acattgcgta aaggagaccg
     1261 tatctacgtt tctccgtcag cggtgcttca atttgagcca ctgtaacttg tcaggttctt
     1321 ctcacacggc cacgttggtt gaccggctat agggcttatt ggggtcgatg agcacattga
     1381 tacatgctgg caacccggat gcgaaagctc tctcaagggc cggacgaata tcttccggcc
     1441 tttgcaccag ctcgccgtaa cctcccaggg cttcgaccat tttgtcgtag cgaatccctg
     1501 gtgagagttc cgctgcggtg gcatggccga ggattgcctt ttgtgggtgc ttaatctgac
     1561 cccatgctcc atcgttgcca atgatcgata caacgggcaa ctgctggcga accatgctct
     1621 cgaactccat gccgttgagc ccaaaagcgc catcaccatg caggatcagc acttttttgc
     1681 ctggccgggc gatctgcgcg gccatcgcga acccggtgcc cgttcccagg caccccatgg
     1741 gtcctggatc gagccagtag cctggtttat cgacattgat gactctgcca ccgtagctga
     1801 cgatatcgcc gccatcgcct acaacggtgg tgtcttcgtc gataaagtcg cgtatctcgc
     1861 ggcaaaaacg catgggatga atgggtacat catctgaact catcagcgct ccatcacgcc
     1921 ccagcgcatt cctttcttct ctgcgaatat aggcaagcca attgccttca tcgagctgcc
     1981 tggttgcctc gctcaattgc tgcatactca accctacatc tcctaccagc ccggcggccg
     2041 cgccgcggtt gacaccgaca tcttcgcccg cgaggtcgaa ccagatcaca ttggcttctt
     2101 tgggaatgag cggtggctgc ccgtgattga gccgaaaatc catgcgcgtc ccgattgcca
     2161 ggatcgtgtc ggccccttcg agggctttgc ggcgggacga cgtaaagaac agcgggtgcg
     2221 taggcggcag gcagccacgt ccagcgccgt tgaggaagac aggggcgttg atgcgctccg
     2281 caagctggcg cagtggttcg gcggcatcac accaccagac ggcggtacca gccatgatga
     2341 ctggacgctg cgctttttcc aacagggcag ccgccttgac gaccatggcg ggatcgggcg
     2401 cggtacgacc gccgtaacga taggatgctg gttcgcctgg atcgaagagg gtatcggtat
     2461 ccgcgaggtt gttcaggaca tccatgggca tttctagaaa ggtggggcca ggtttgccgt
     2521 tcaacatctg gcgaaaggcg gaggcggtgt actcgcccag gcgtgctgtc tcatgcacgg
     2581 tgcgcgccca cttggtgatt gggcgcagca ggtcaatctg gttcatttcc tgtagcgcgc
     2641 ctcgatccca caggctcaaa ggggcggctc cgcctatgac gaggatgggg ctgcccgcga
     2701 ggtaggcatt tgctacgccg gttacgccgt cggttacgcc cgggcctgct gttaagagcg
     2761 caactcccgg agtgcgcgtg catttggccc agccttcggc ggcgtgaacc gctgcctgtt
     2821 cgtggcgagt atcgacaacc ctgattcctt cgcgtttgca cccatcatag atggcagcga
     2881 tatgcccacc gctgagggtg aagacgactt cgactccttc tcttttgaga aatttggcga
     2941 cgagccagcc gccgtggatc atgcccatat cgtactcaac tcctttttat ggctaccgct
     3001 tcagaacgac aaccgcatcc cagagcctcc agggcgacca caagggtgcg cccctactat
     3061 ggacgaccct tgtcaggcgc tccgcaggca tagtaggggc gcacccttga ggtcaatacc
     3121 gcttaaataa gtggaagagg agcttcgaat ctgacatact aagaaaaaac cacgatctgg
     3181 tatgtcgaaa ggagacccct catgcccttt agaataacgc acctgcacag cgctgacaaa
     3241 ctgtgtcgca ccctgacctg gccgatcttg gaacacctct atccccgcca gcgcattgag
     3301 cacctcgtcg agaccttctg cggccagacc actcgtgtgc gcaagctgag tggagtgctg
     3361 accgtttggg tgctcatctg ttggcacctg tatttgcgcc acagcttggg tgccgtcttc
     3421 ctcaagctca gtagtgccga gcgttggctg gccgaggacg agccggagtc gccgcccacg
     3481 cgcgctgcct ggacctatcg gcgcaaacaa ctcggcgtac ggctcctgcg tgccctcttt
     3541 gaagagtcgt gtgtgccgct ggccacccaa gacacaccgg gtgccttcgc ctttggcttg
     3601 cggctgatgg ccattgatgg caccctggaa gacgtcaacg atactcccgc taacgctcag
     3661 tattttgggc gcatctctga cggccagacc agcagtccct atccgcaact gcggtgcatc
     3721 tacctgctcg aagctggcac ccatgccatc gtcaaggtca tcgccgctcc ctgcaaggcc
     3781 tccgaacagt gcttggcacg tggtttgctg cctgcgatca aaccaggcat gctcgtgctg
     3841 gtcgatcgcg gctttgtctc gggagccttg ctggaggcca tttgtgcgcg tggcgctcag
     3901 gtgctgggtc gcctgcctca aggtatcttt atgcacaaag aac
//
LOCUS       DASZAK010000697         6234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_409210, whole genome shotgun sequence.
ACCESSION   DASZAK010000697 DASZAK010000000
VERSION     DASZAK010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6234
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409210"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VGT44_12305"
     CDS             complement(<1..697)
                     /locus_tag="VGT44_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEV2581626.1"
                     /translation="MTDQAHIRNFCIIAHIDHGKSTLADRLLEYTGTVSKRELMEQTL
                     DQMDLEREKGITIKAQAVRMLYTALNGEQYELNLIDTPGHVDFTYEVSRSLAACEGAL
                     LVIDATQGIEAQTLANLYLALEADLAIIPVINKIDLPSADPDRVRQEVEDVIGLPAED
                     CILASAKEGIGTQDILEAIIAQIPPPKGNLEHPLRALVFDSHYDSYKGVIAYVRIVDG
                     MLREGEKIVMMATG"
     gene            complement(694..1866)
                     /gene="mnmA"
                     /locus_tag="VGT44_12310"
     CDS             complement(694..1866)
                     /gene="mnmA"
                     /locus_tag="VGT44_12310"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF001138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV2581627.1"
                     /translation="MARIVVAMSGGVDSSVAAALLKEQGHEVIGMMLRLWSEPALIED
                     DGVERVVQNKCCSLESVDDARRVARMLDIPFYLVNVEQEFKEHVVDFFYAEYMAGRTP
                     NPCLTCNRHIRFTLLLNRALALDADYLATGHYVRVDQNPATGKFRLRRGIDAGKDQSY
                     VLHVLNQQQLAHACFPLGEYTKPQVRAMAAERGMSVASKAESQEICFVAQNDYRGFID
                     RYAKAQNEEQQELVGASVATLTRPAGQTIQVPRSGPIIDTSGKTLGRHRGLAYYTIGQ
                     RKGLGLTSTEPLHVLKIDAEQNTLVVGPAEGLLQSACTVGKMHYLNGETPTAPFTASI
                     RIRYKSPEQEAIVTPLDGNRARVTFERPQRAVTPGQAAVFYGGEEGDEVLCGGIIE"
     gene            complement(2032..4923)
                     /locus_tag="VGT44_12315"
     CDS             complement(2032..4923)
                     /locus_tag="VGT44_12315"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV2581628.1"
                     /translation="MRKLPTNTITLLFTDIEGSTRLLQLLGERYGDLLMECRRLLRDV
                     FQHWHGHEVDTQGDAFFVVFARAADAVSAAVAAQRALAAHAWPDGVEVRIRMGLHTGE
                     PSLVAEGYVGEDVHYAARIMSSAHGGQVLLSLTTRELVEHALPDGVGLRDLGEHRLKD
                     LGHPIHLFQLVISDLPADFPLLKTLGTHPNNLPTQPTPFIGREKEVAVVGQLLCREDV
                     HLVTLTGPGGTGKTRLALQVATALSDLFADGIYFVDLAPVSDPALVIGAIAQSLSIRE
                     VSSQPLLERLEDELQRKQLLLLLDNFEQVVGAAVQVADLLTTCPQLKVIVTSRVVLHV
                     QAEQEFFVPSLSLPDHNHLPELTAFSQYESVALFIRRAQNVRSDFQVSNANAPAVAEI
                     CVRLDGLPLAIELVAARMKLLTPEALLARLGQPLHVLTSGPRDVAARQQTLRNTIEWS
                     YQLLDAQEQQLFRRLSVFVGGCTLEAVEALCASLGDETESGLDTAASLADKSLLRRME
                     QQAVQEPRFATLETIREYALERLAASGETEAARRAHAAYFLRLAEEAEQGMVGPQQAV
                     LLERLEQEHDNLREALEWALEKETDEQAAERREIGLRLSAALKEFWMMHGHYREARTF
                     LERALALSEGTSAALRARVLRAIASVADCQGDIDRIEAVAQQSLTLSRELADTCGIAE
                     SLGSLAAAAWLRGKIVEAVSLHEEQVRLLRQVGEPGEVADALFPLAEHVSTHGEYDRG
                     QALFEEALQLFRQARNELWIGGTLVHSASWLWFTLGDRATMRQRFQEGQALITRVGDR
                     AWSAECLWVAALLALSEAEPVRASSLAQESLSIYREMGDPWYSAWLLHLLGRIETQRG
                     ELPAARTYYQQSLALNQQVGEKWMTPFNLEGLAGVVATQGALRWAAQLWGAAEALREA
                     IDVPRLPVDRRSYELAVAAARAQLGEEAFTIAWQEGRTMSPEQALAASA"
     gene            5134..>6234
                     /locus_tag="VGT44_12320"
     CDS             5134..>6234
                     /locus_tag="VGT44_12320"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2581629.1"
                     /translation="MSEQTRQQPLAPVPGRRTFLIRRRLILLVGLAFLLVAASVGLLT
                     PLIPSLFRTTGASPSLASITNIAVPAMFGIDAQNTRVVPDEQRLTYANVSHLVADWTS
                     LPTGGSIFSSPVVANGVVYVGSFDGRLYAFDAAGCGQARCAPLWMSAPTGDRIFATPA
                     VANGVVYIGSYDRKLYTFRADGCGAFTCSPLWVSTPLGDAIDSSPTIANGTVYLGADD
                     GKLYAFQAAGCGRTTCQPLWTTIASRPAVVSPPAVADGLVYVGTYDPVSGAGSLFAFN
                     AGGCGKPACSPLWSATPGIYDDGYNAAPAISNGFVYAASLDGTLKAFNAKGCGRATCA
                     PLWSASAGDFIESSPAVTGWVVYIGSWDHRVYA"
BASE COUNT         1144 a         1929 c         1793 g         1368 t
ORIGIN      
        1 taccggtggc catcatgacg attttctcgc cttcgcgcag cattccatcg acgatacgca
       61 cgtaggcgat cacgcctttg taggaatcgt agtgtgaatc gaagaccagc gcgcgcaatg
      121 gatgctccag attgcctttc ggcggcggga tctgtgcgat aatggcctcc aggatgtcct
      181 gtgtgccgat gccctctttg gccgaggcca gaatgcaatc ttcggcgggc aggccaatga
      241 catcctcgac ctcctgccgc acccggtcgg ggtcggcgct gggcaggtcg atcttgttga
      301 tgacagggat aatggcgagg tcggcttcga gcgccaggta gaggttcgcc agcgtctgcg
      361 cctcgatgcc ctgcgtcgcg tcgattacca gcagcgcgcc ctcgcacgcg gccaggctgc
      421 gcgagacctc gtaggtgaaa tcgacatgcc cgggcgtatc gatcaggttg agttcgtact
      481 gctcgccgtt cagcgcggta tagagcatac gtaccgcctg cgccttgatg gtgatgcctt
      541 tttcgcgttc caggtccatt tgatcgagcg tttgctccat caattcacgc ttggagacgg
      601 tgccggtgta ttcaagcaat cgatcggcca gcgtggattt accatgatcg atgtgcgcga
      661 tgatgcagaa atttcggata tgagcctggt ctgtcattca ataattcctc cacacaacac
      721 ctcatcaccc tcctcgccgc catagaagac ggctgcctgt ccaggcgtga cggcgcgctg
      781 tgggcgttca aaagttacac gcgcgcgatt gccgtccagg ggcgttacga tcgcctcctg
      841 ttccggcgat ttgtagcgta tgcgtatgct ggccgtgaag ggagcggttg gcgtctcgcc
      901 gttcaggtag tgcatcttgc cgaccgtgca ggcgctttgc aaaagtccct ctgccgggcc
      961 gaccaccagt gtattctgct ccgcgtcgat tttcaggacg tgcagcggct cggtcgaggt
     1021 caggccgagt cccttgcgtt gcccaatggt gtagtaggcc agcccccggt gcctgcccag
     1081 tgtcttccca ctcgtatcga taatcgggcc ggagcgcggt acctggattg tctgaccggc
     1141 ggggcgtgtc agcgtcgcga cgctcgcgcc taccagctct tgctgttcct cgttctgcgc
     1201 tttggcgtag cgatcgataa agccgcgata gtcgttttgc gccacgaagc agatttcctg
     1261 gctctcggcc ttgctcgcca cactcatgcc gcgctcagcc gccatcgcgc gcacctgcgg
     1321 cttcgtatac tcgcccaggg ggaagcaggc atgggccagc tgctgctggt tcagcacgtg
     1381 caggacatac gattgatctt tcccggcatc gatgccgcgc cgcaagcgga acttgcccgt
     1441 cgccgggttc tgatcgacac gcacgtagtg acccgtcgcc aggtagtcgg cgtcgagcgc
     1501 cagggcgcgg ttcagcagca aggtgaatcg aatgtggcga ttgcaggtca ggcagggatt
     1561 gggtgtgcgc ccggccatat attccgcgta gaagaaatcg acgacatgct ccttaaactc
     1621 ctgttctacg ttgaccaggt agaaaggaat gtcgagcatg cgcgccacgc gccgcgcatc
     1681 gtccaccgac tcaagcgagc agcacttatt ctggacaacg cgctcaacac cgtcgtcctc
     1741 gatcagcgca ggctccgacc acaggcgcag catcatgccg atcacctcgt gtccctgctc
     1801 tttgagcagc gccgctgcta ctgaactgtc tacgccgccg ctcatcgcga cgacaatcct
     1861 tgccattgtc gaattgctcc cttgcgcaag aaaatgacta gccgcgcggg ctagtctgca
     1921 tgtgttgcgt ctacgttgtt gtgtgctgct gtgctggccg gtatcgcccc ctttgtgcca
     1981 cgttcagagt aacatagatg ggccatcttg tcaaattaac cattgaggcg atcacgccga
     2041 ggcggcgagg gcttgctctg gcgacatcgt gcgtccctct tgccacgcaa tggtaaaggc
     2101 ttcctctccc agttgcgccc gtgcagcggc caccgccagt tcatagctgc gtcgatcaac
     2161 gggcagccgg ggaacatcta tggcttcgcg cagggcttct gctgctcccc acaactgtgc
     2221 tgcccatcga agtgctccct gggtggcgac cacaccggcc agtccctcca gattgaaggg
     2281 ggtcatccac ttctctccta cttgctggtt gagcgccaga ctctgctgat aatatgtgcg
     2341 agctgcgggc agctcacccc gctgtgtctc aatcctacct aacaggtgca gcaaccaggc
     2401 actataccag gggtctccca tctcccgata gatactcagg ctttcttgag ccaggctgga
     2461 tgctctgacc ggctctgcct cagagagggc cagcagtgcg gcaacccata agcattcagc
     2521 actccaggct cggtcgccca ccctggtgat gagcgcctgt ccctcctgga accgctggcg
     2581 catggtcgcc cggtcgccca gcgtaaacca gagccaggag gccgagtgga cgagcgtgcc
     2641 accgatccag agctcgttgc gtgcttgtcg aaagagctgg agcgcctcct cgaagagcgc
     2701 ctgtccccgg tcgtactccc catgcgtgct gacgtgctct gccaatggga agagggcatc
     2761 ggcaacttct cctggctcgc ccacttgtcg caggagtctc acctgctctt catgaagcga
     2821 gacggcctcg acgatctttc ctctcaacca ggcggcagct gccagtgagc cgagggactc
     2881 agcaatgccg caggtgtccg ccagttctcg ggagagggtc aggctttgct gggccaccgc
     2941 ctcgatccga tcaatgtcgc cctgacaatc tgcaacagat gcaatagccc gtaaaaccct
     3001 ggcacgcaag gcagcgctgg tcccttcgct caaagcgagg gcgcgctcta gaaacgtacg
     3061 agcctcacga tagtgtccat gcatcatcca gaattccttg agcgccgcgc tcaagcgcaa
     3121 gccgatctcc ctgcgctctg ccgcctgttc gtctgtctcc ttctccagcg cccactccag
     3181 ggcttcgcgc agattgtcgt gttcctgttc caggcgctcc agcaagaccg cttgctgtgg
     3241 tcctaccatt ccctgctctg cttcctctgc aagtcgcagg aagtaagcag catgagccct
     3301 tcgcgctgcc tctgtttccc cgctagcagc caggcgctcc agcgcatact cacgaatcgt
     3361 ctccagcgtg gcgaagcgtg gctcctggac cgcttgctgc tccatgcgtc gcaacaggct
     3421 tttgtcggcg agggaagctg ctgtgtccag acccgattct gtttcgtcac caagtgaagc
     3481 gcaaagtgcc tctacggcct ccagggtgca gccaccgaca aaaacggaga gccgtcggaa
     3541 gagctgttgc tcctgggcat caagcaactg gtagctccac tcgatcgtgt tacgcagggt
     3601 ctgctgccgc gctgccacat cgcgcggccc acttgtcaac acgtgtaggg gttgacccag
     3661 tcgtgcgagc agcgcttcgg gcgtgagcag tttcatgcgc gccgccacca gttcgatcgc
     3721 cagtggcagt ccatccaggc gcacacagat ctccgccaca gcaggagcgt tggcattgct
     3781 cacttgaaag tcagacctga cattttgcgc tcgccgaatg aacagcgcta cagattcgta
     3841 ctgggagaac gccgtcagct cgggcaaatg gttgtgatca gggagtgaga gtgatggcac
     3901 gaagaactct tgctcggcct gtacgtgcaa tacgaccctg ctggttacga tcactttgag
     3961 ttgtgggcag gtggtaagga ggtctgccac ctgcactgcc gcgccgacca cttgctcgaa
     4021 attgtccagg agcagcagta gttgcttgcg ctgcaattcg tcctccaggc gttctaagag
     4081 aggttgacta ctcacctcgc ggatgctgag gctctgtgcg atagctccga tgaccagcgc
     4141 aggatcgctt actggagcca gatcgacgaa atagatgcca tcggcgaaga gatcgctcaa
     4201 tgctgttgcc acttgcaagg ccaggcgcgt cttccctgtt ccgccggggc ctgtcagcgt
     4261 caccaggtgc acatcctcac ggcacaagag ctgtccgacg acagccactt ccttctcccg
     4321 tccaatgaag ggcgtgggct gagtaggcaa attgttgggg tgggtaccga gcgttttgag
     4381 caatgggaag tcggcaggca ggtcggagat cacaagctgg aagaggtgaa tgggatgtcc
     4441 caggtccttg aggcgatgct cacccagatc ccgcaagccc acgccgtccg gtaaggcgtg
     4501 ctctacaagc tcccgcgttg tcagggacag cagcacctgc ccaccgtgag cggaactcat
     4561 gatgcgcgcc gcgtaatgca catcctcacc cacatagcct tcagctacca gagagggttc
     4621 acccgtgtgc aaacccatcc ggatacgcac ctctacgcca tccggccagg cgtgagcagc
     4681 aagagcgcgt tgtgccgcca ctgctgccga gacagcatcg gcggcacgag caaacaccac
     4741 gaagaaggca tccccttgcg tatccacctc gtggccatgc cagtgctgga acacgtcgcg
     4801 cagcaggcgc cggcactcca tcagcaggtc gccatagcgc tcacccagga gctgaagcag
     4861 gcgcgtcgac ccctcaatat cagtgaaaag caacgtgatt gtattcgtcg gcagcttccg
     4921 catgaccacc ttctctgctt gacgatgatg atacagactc aggtgatttt attatatcgt
     4981 atctctctgt cgtgtgatgt ggcctgggtc tgtcaaaggc atagacatat ttctcccttt
     5041 tgagaggaga aatatgttgc gcaatacatc ctcttctcag gcgtctctac aatagcggtc
     5101 cgttcactcc aaccacatcg gaggatagct gagatgtctg agcaaacccg gcaacaaccg
     5161 cttgctcctg tccctggtcg tcgcacattc ctgatacggc gcaggcttat cttgctcgtg
     5221 ggtctcgcgt ttctacttgt tgctgccagc gttggccttt taacaccgct catcccttca
     5281 ttgtttcgta caactggcgc ctcaccatct ctagccagca tcacgaacat cgctgtccca
     5341 gcgatgtttg gtatcgatgc ccagaatacc cgtgttgttc ctgatgaaca acgcctgacc
     5401 tatgcgaacg tttctcacct cgttgctgac tggacctcgt tgccgacggg cggaagcatc
     5461 ttttcttcgc cggtcgtcgc caatggcgtt gtgtatgttg gctccttcga tggcaggctt
     5521 tatgcctttg atgcagccgg gtgcgggcag gctcgctgcg cgccgctgtg gatgtctgcg
     5581 cctacgggcg accgcatctt cgccacaccg gccgtcgcga acggcgtcgt ctatatcggc
     5641 tcgtatgatc gcaaactcta tacctttcgc gccgatggat gcggcgcttt cacttgctcg
     5701 cccctctggg tatcaacccc gcttggagat gcgatagatt cctctccgac catcgcgaac
     5761 ggcacggtgt atcttggcgc ggacgacggg aaactctatg cattccaggc tgctggctgt
     5821 ggccgcacaa cgtgtcagcc gctctggaca accatcgcaa gtcggcctgc tgttgtctcg
     5881 ccgcctgccg tcgcggatgg tctggtctat gtagggactt acgacccggt cagcggagcc
     5941 ggttcgctct ttgctttcaa cgctggtgga tgcgggaaac ccgcctgttc tcctctgtgg
     6001 agcgctacac cggggatcta tgacgacggt tataatgccg cgccggcgat atccaacggt
     6061 ttcgtctatg cggcttcact ggacggtacc ttgaaggcgt ttaacgcgaa gggatgcggc
     6121 agagccacct gcgcgccgtt gtggagcgcg tcggcgggtg attttatcga atcgtctccg
     6181 gctgtcaccg gttgggtggt ctatatcggt tcgtgggatc accgggtgta tgcg
//
LOCUS       DASZAK010000698         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_479366, whole genome shotgun sequence.
ACCESSION   DASZAK010000698 DASZAK010000000
VERSION     DASZAK010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479366"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..393
                     /locus_tag="VGT44_12325"
     CDS             100..393
                     /locus_tag="VGT44_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581630.1"
                     /translation="MPKSIEGVSREEMLQHAAEEIRAVWELYAQGICREFYTRINEPG
                     RVVMMLESASEEAAREALAMLPFAKLHLIDFDIIPLGPFMGLTRLFQSPVEAQ"
     gene            404..811
                     /locus_tag="VGT44_12330"
     CDS             404..811
                     /locus_tag="VGT44_12330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV2581631.1"
                     /translation="MMSKFTDIELEYLRSQRLGRLATINKNGEPQIAPVTFLYNAELD
                     AIDIGGLYNGESQKFRNVARNGLASFVIDDVLPPFQPRGVEIRGRAEALPTGGQSISP
                     NFSPALIRLTPGRILSWNLISGDAAQSHSARDV"
     gene            834..1709
                     /locus_tag="VGT44_12335"
     CDS             834..1709
                     /locus_tag="VGT44_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEV2581632.1"
                     /translation="MVVSFESYRSYLFAIAYRMLGSAMDAEDMVQETYLRYQTASPET
                     IISLKAYLTTIISRLCVDQLQLAHRKREQYLGPWLPEPIITAEASELARVEERVDREE
                     SISLAFLVLLEQLQPVERAVFLLREVFDYDYAEIATFLGKSEAACRQWFSRAKKHLGD
                     HRPRFPASPETQKQLLTGFVRAVQAGEMSSLMNLLAEDVAFYGDGGGKVKGAATHPIA
                     GRMAVARFFLETSSLFRSALPDGSHIELAEVNGQPALITRAGNRAFAVLTIEVEAEHI
                     QTIRIVANPEKLAHV"
BASE COUNT          413 a          470 c          499 g          396 t
ORIGIN      
        1 cttgaccctc ttgctttatg atgatcttcc tgatgaggat cacgacaacg tttctaccaa
       61 ccagaaattt ggaggttctg tcctatgaag attttagtca tgccaaagtc aatcgaaggt
      121 gtctcacgcg aagagatgct acagcatgcg gcggaagaaa ttcgagccgt gtgggaactc
      181 tatgcccagg gcatctgccg cgagttttac acccgcatca acgaaccggg gcgggtcgta
      241 atgatgctcg aaagtgccag cgaggaagct gccagagagg cgcttgccat gctgccattt
      301 gcgaagttac acctgatcga ctttgacatc atcccactgg gtcctttcat gggcctgacg
      361 cgcctgtttc agtccccggt cgaggcacag taagggaggt aaaatgatga gtaagtttac
      421 ggacatcgaa ctggaatact tgaggagcca acggctcggt cggttggcaa ctatcaataa
      481 aaatggcgaa ccgcagatcg cccccgtgac cttcctatac aacgccgaat tggacgctat
      541 cgacatcggc ggattgtata atggtgagtc gcagaagttt cgcaatgtgg cgcgaaatgg
      601 cctggcgtct ttcgtcatag atgatgtatt gccgccgttt caaccacgcg gcgttgagat
      661 tcgcgggagg gcggaagccc tccccacagg tgggcaaagc atttcaccaa acttcagtcc
      721 ggccttgata cgcttgacac cagggcgtat tctctcctgg aatctgattt ctggcgatgc
      781 cgctcaaagt cattcggcgc gcgatgtcta atagacagtt aaggaggatg aacatggtgg
      841 tatcgtttga aagctaccgg tcgtacctgt tcgctatcgc ctaccgcatg cttggcagcg
      901 cgatggatgc cgaggatatg gtgcaggaaa cctacttgcg ctaccagacc gcttcgcccg
      961 aaaccatcat ctcgctcaaa gcctatctca ctaccatcat cagccgcttg tgtgtagatc
     1021 aacttcaatt agcgcatagg aagcgagaac agtacctggg tccctggctg ccagagccga
     1081 tcatcacggc agaggcttcc gaactggcca gggtggagga gcgcgtcgat agggaggagt
     1141 cgatctcgct ggcgttcctg gtgctgctgg aacaactgca acccgtcgag cgagccgtct
     1201 tcctgctgcg cgaggtcttc gactacgact acgccgagat cgctaccttt ctcggcaaaa
     1261 gtgaagcagc ctgtcgccaa tggttcagtc gggcaaagaa gcatcttggc gaccaccggc
     1321 cacgcttccc agcctctcca gaaactcaga agcagctgct cacgggtttt gtgcgggcag
     1381 tgcaagccgg tgagatgagc agtttgatga acctgctggc cgaagatgtc gccttctatg
     1441 gtgatggtgg cggcaaggtc aaaggcgcgg cgacgcaccc cattgctgga cgtatggcgg
     1501 tggcgcgctt ctttctggaa accagtagcc ttttcaggag cgcgttaccg gatggttctc
     1561 acattgagct ggccgaagtc aatggtcaac cggcgctcat tacacgcgcc ggtaatcgcg
     1621 cctttgccgt actgacgatt gaagtggagg ccgagcatat tcagactatt cgtattgttg
     1681 ccaacccgga gaaattggca cacgtctaac ggacaacttc caaggataaa gcatcgacat
     1741 aaccgatggt taggaggcaa ttacaacggt acactgga
//
LOCUS       DASZAK010000699         3046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_657845, whole genome shotgun sequence.
ACCESSION   DASZAK010000699 DASZAK010000000
VERSION     DASZAK010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657845"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGT44_12340"
     CDS             complement(<1..384)
                     /locus_tag="VGT44_12340"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2581633.1"
                     /translation="MQASLCLFPAGFRDDAMTPRLRKRLFETLYKNKYLYRFASTVPF
                     AGQWRAWQRLVLPRLVGHDVLELGCGLGDLLADMLAAGYTCHAVEASPQMVAAARETL
                     ARRKVGDQGLVIQGSARGLPFSDGSF"
     gene            complement(457..1122)
                     /locus_tag="VGT44_12345"
     CDS             complement(457..1122)
                     /locus_tag="VGT44_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEV2581634.1"
                     /translation="MGSIFNPNDPRWVKDTREVMEPRGIIVPNVIESTPRGERGMDIY
                     SRLLKERIIFIGTPIDDNVANLTVAQLLFLQSEDATKDIWLYINSPGGVIYAGLAIYD
                     TMQWLKPDVATVCVGMAMSMGAVLLASGEKGKRYCLPNSTVLIHQPLGGAEGQAADIE
                     ITAREMLRLRTSIYDILSERTGQPRERIMQDADRNYYLTAPQAVEYGLVDEILSNEDA
                     LTR"
     gene            complement(1353..2882)
                     /locus_tag="VGT44_12350"
     CDS             complement(1353..2882)
                     /locus_tag="VGT44_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2581635.1"
                     /translation="MERLNDIFKRDAHQLPRRRPEGNERQVAPARPTTPAASPASQQM
                     RPGSGPAGTPTRRPLPEQTARIGGYSRAYQSSAFTPSPPSPALPVPSVPATPANRQPP
                     GHQDAYGITHSQTRTQASRPDYQGDAYQPAAGADVQREWREEWDDEEAVSIRYGDWES
                     DIYDMPALPTIAERQREQEAHPPVSRGANRIAQTLPQGSRSLVTRELRRPVDEEELPA
                     HYAPQKRQPMQSSPAPLSPPRAPETALARIQRATQPLNRSPRMDRAERIEPEPIPAPY
                     TSPKATCPLCKGKGYLRADVPYGHPNFGRPIACECKEKERRERRRLQLQEMSNLSELR
                     DKQFDNFKSNVSRVVYEAYQIAREYAEEPEGWLVLIGRVGCGKTHLAAAIANQALANG
                     SRVLFSTVSDLLDHLRATFMPNATEFYDHLFQEMREAELLILDDLGAQQSSPWANEKL
                     FQLLNYRYNSRFPTIITTNNTGLQGIEERIRSRIMDVGLVTTVAFDQTGDYRMRNPRR
                     D"
BASE COUNT          581 a          889 c          902 g          674 t
ORIGIN      
        1 gaaagagcca tcgctgaacg gcaacccccg cgccgaaccc tggatcacca ggccctgatc
       61 gcccactttg cgacgagcca gcgtttcgcg cgccgccgcg accatctgag gcgaggcttc
      121 gacggcatgg caggtgtacc cggctgcaag catgtccgcc agcaaatcgc ccagcccgca
      181 gccgagttct agcacatcat gccccaccag gcgcggcaaa accagccgct gccaggcgcg
      241 ccattgcccg gcaaacggca ccgtgctggc aaaacggtaa agatatttgt ttttatacag
      301 cgtctcgaag agccgttttc gtagtcgcgg cgtcatagcg tcatctctaa aacccgccgg
      361 aaacaggcat agggacgctt gcatcgccgt catacctgtt ggaatggtgg tatgattcaa
      421 caggtatgac ggcggcgcaa gcgtccctat gcctacttag cgcgtcagcg catcctcgtt
      481 cgagagaatc tcatcgacca gtccatactc aacagcctgc ggggcggtca ggtagtagtt
      541 gcggtcggcg tcttgcatga tacgctcgcg cggctgtccc gtccgctcgg aaaggatgtc
      601 gtagatgctg gtgcgcaggc gcagcatttc gcgcgcggtg atctcgatat cagccgcctg
      661 gccttccgcg ccgccgaggg gctggtggat cagcacggtc gagttgggca agcagtagcg
      721 tttgcccttc tcaccggaag ccagcagcac cgcgcccatg ctcatcgcca taccgacgca
      781 gacggtcgct acatcgggct tgagccactg catggtatcg tagatggcaa gaccggcgta
      841 aatcacgccg ccaggcgagt tgatgtacag ccagatgtct ttggttgcat cttcgctctg
      901 gaggaaaagc aactgcgcga cggtcaggtt agcaacatta tcgtcaatgg gcgtgccgat
      961 gaagatgatg cgctccttga gcaggcgcga atagatgtcc atgccgcgct cgccgcgagg
     1021 cgttgactcg attacattcg ggacaatgat gcccctgggt tccataactt cgcgggtgtc
     1081 tttgacccac ctgggatcgt tcgggttgaa tatgctaccc atctcagctc cttatgctta
     1141 taagaaacga ttggccttcc tgctcacttt gcctgcctcg cggcctgcaa gtctcgcgta
     1201 gaaggaatga tcggctaaca agaaatgtgc agaagtacgc tgtactttta tagtacagag
     1261 ccacgtaccg ttatgatagc acctgcaact gataaacgca agcaaaatga ggggcaattt
     1321 ggcactctca caccgggtct gctaattctg gcctaatccc ttctcgggtt acgcatgcgg
     1381 tagtcgccgg tctggtcgaa tgcgacggtc gtcaccaggc cgacatccat gatgcgcgag
     1441 cgaatgcgct cttcgatacc ctgcaagccg gtgttgttcg ttgtgataat cgttggaaag
     1501 cgcgagttat agcggtaatt gagcagttgg aagagctttt cgttggccca gggcgaactt
     1561 tgctgcgccc ccaggtcgtc gaggatcagc agttccgcct cgcgcatctc ctggaaaagg
     1621 tgatcgtaaa actcggttgc attaggcata aaggtggcgc gcaggtgatc gagcaggtcg
     1681 ctgacggtgc tgaacagtac acgcgatccg ttcgcgaggg cctggttggc gatggcagcc
     1741 gccaggtgcg tcttgccgca gccgaccctg ccgatcaaga cgagccagcc ctccggttcc
     1801 tctgcatact cgcgcgcgat ttgatacgct tcatagacga ccctggacac gttggatttg
     1861 aaattatcga actgtttgtc tcgcaattcg ctgagatttg acatttcttg cagttgcaag
     1921 cgtctgcgtt cgcgccgctc tttctcttta cattcgcatg caatcggcct gccgaaattg
     1981 ggatggccat atggcacatc ggcgcgcaag tagcctttgc ctttgcagag cgggcaggta
     2041 gccttcggac tggtataagg cgcgggtatc ggctccggct ctattctttc cgccctatcc
     2101 attctcggcg agcgattaag cggttgggtg gcgcgctgga tacgcgccag cgccgtctct
     2161 ggcgctctgg gtggactcaa tggcgctggc gatgattgca tcggttggcg cttctggggc
     2221 gcgtagtgcg ctggcaactc ctcttcatcg accggcctgc gcagttctcg cgtgacgagc
     2281 gaccggcttc cctggggaag ggtctgcgca atcctgttag ccccacgact caccggtgga
     2341 tgcgcctctt gctcgcgctg gcgctccgct attgttggta gcgcgggcat atcgtagata
     2401 tcgctttccc aatcgccata gcggatggag actgcttcct catcatccca ttcttcccgc
     2461 cattcacgct gaacgtcagc gccagcggcg ggctggtagg catctccctg gtaatcagga
     2521 cgactagcct gcgttcttgt ttgcgaatga gtgattccgt aggcatcctg atgaccgggc
     2581 ggctgcctat tcgccggggt agccggaacg gacggcacag gtaacgcagg tgacggagga
     2641 gatggagtga atgccgacga ctggtacgcg cgtgaatatc ccccaatgcg cgccgtctgc
     2701 tctggcaagg gacggcgtgt tggagttcca gcagggccag aacccggacg catctgctgc
     2761 gacgcggggg atgccgccgg tgttgttggc ctcgcaggtg ctacctggcg ctcattgccc
     2821 tccgggcggc ggcgcggtaa ctggtgcgca tctcgcttaa aaatatcgtt caggcgttcc
     2881 atgtctgcct gccttccgtt tcccagcgcc gtaaaatagc gcgaatataa ctccacttcc
     2941 gtttattgtg caagacggcc tcgcgaaacg cggccttgat ccactcaact ggatactgct
     3001 cggctgcgtc cttcagctcg tcggcgatca ggggtgagag taatcc
//
LOCUS       DASZAK010000700         4221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_373997, whole genome shotgun sequence.
ACCESSION   DASZAK010000700 DASZAK010000000
VERSION     DASZAK010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373997"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..1465
                     /locus_tag="VGT44_12355"
     CDS             131..1465
                     /locus_tag="VGT44_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2581636.1"
                     /translation="MQQQRRGQAIGHTRSPTLTKAVTHIRLGAANAGKLAALDALAEV
                     YLALCQQYVTRFCTEEQPDKFRPTCFATPLSERWHRVAIQQAAGIAQSWRANRAQAYQ
                     DYLDDLLDAHEQEAEGLLDAETKAPVWREWSIPVLRQTCIQANANVVALEPSQDSTFD
                     YWLKISTLERSKTTRLPVKLAEYHREALVGKTIHTSVTLNRRDAVWWVTLSYDEVVPL
                     QTPPSAPVVGIDVGVANFLTTSDGKRYGTFHGKLRERQKRDREKRRRKAKLRACLEKK
                     GVQKLPSTTSRGAQRLARHVRREINRAVNQCFAAHEGARVAYEHLSVATMKHKARAMN
                     AYLRASNLAHLPKQIEWTAAKRGILATRVKSAYSSQACSVCYYVDRANRPNQQTFCCR
                     VCGYRAHADVNAATNIERRVGDTELRACKDRKAVKALLMHRHQAWKKQNGWP"
     gene            1688..3769
                     /locus_tag="VGT44_12360"
     CDS             1688..3769
                     /locus_tag="VGT44_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV2581637.1"
                     /translation="MEHQSVTKQAAKVSGSDEQSHDRNLLGPLLCWAVVFADIGTSVY
                     YTPGILYQTVGKLAGFFVLLTMTVFVLLTLKYAEVTHRFPQGGGVVTVAAQAINHWVG
                     ALGGMFILVDYFLTAAISCLSGMIYLSVVVPAIGPIALWITIIVLVLLGLLNWVGISE
                     SAKVSLVGAVIAFLSDLVILYTVFTHISLGQFFALFGEMFRGNPLTPTSILVGFAGSF
                     LAFSGLESISQLSPVMRTPRKRVAGIALLLVVLTIGLTSPLLTMLSTLLVTNSAGHPL
                     ATDPVLSGQLISLLGGKWGGVILGTEVAISASALLVFASNTAIIGAYHVFMALSRMEF
                     FPAVVLKRNKLRGTPHYSIALATGIPIIILIIVRGNINILGDMYAFGLLGAFTLTCLG
                     LDIVRHRERRLARRLSKRIRHDRLNGRTDGTAEKLVESALHEVNSSDHQPYSSNNHGH
                     DRNGVEEPIVGGIELSKVASLAAPRKSLRAWVRDAWYNLDYWLGVMTTALVFIAWSTN
                     LVSKPLATAFGGTVATLGMAVAYSNYARQRRRGRLPVISIGVEGRLPDSILAVLTAGN
                     GQNEMVIRSAIKSADGKPVIFLYVGEPQTNRRPQMFEVFDPYLEDAHAKEYFGKAEQL
                     ASKYNVNRRFLYRQQEPGVVSEVWQVMHPHDTIIAAQNVGDVKEVNPDRIRYELVPGG
                     GGKVAHLLKRW"
     gene            3840..>4221
                     /locus_tag="VGT44_12365"
     CDS             3840..>4221
                     /locus_tag="VGT44_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV2581638.1"
                     /translation="MQDRIREILSWYTSENPGVRTNLARMLNHGRLGGTGKMVILPVD
                     QGFEHGPARSFAPNPAGYDPRYHFQLAIEAGCNAYAAPLGFIEAAVSDYCGEFPLILK
                     CNNHDILNDERDPISAVTAGVNDAL"
BASE COUNT          859 a         1250 c         1249 g          863 t
ORIGIN      
        1 gtccgcgctg ttgttgcgcc agccaggggt gggtgaagtg gccctaagtg ccagcgcctt
       61 acccgccaat ccaggccaga ttcgcctggt ggtcttgtac cgtcctctct gagccgaaca
      121 ggaggcagtc atgcagcagc agcgccgggg ccaagcaatc ggccacacca ggtcaccaac
      181 gctcaccaaa gccgtgaccc acatccggct gggggcggcg aatgccggca agctggcggc
      241 gctcgatgcc ctggccgaag tctatctcgc gctctgccag cagtacgtga cccgcttctg
      301 caccgaggag cagccggaca agttccgccc tacctgcttc gccacgccgc tttctgagcg
      361 ctggcaccgc gtcgcgatcc agcaggcggc tggcattgcg cagtcgtggc gcgccaaccg
      421 cgcccaggcg taccaggact atctcgacga tctgctggac gctcacgagc aagaggcgga
      481 gggcctcctg gatgccgaga ccaaagcacc cgtctggcgt gagtggagca tcccggtcct
      541 gcggcagacc tgcatccagg ccaatgccaa cgtcgtcgcg ctcgaaccat cccaggactc
      601 cacctttgac tattggctca agatcagcac cctcgagcgc tccaagacca cgcgcctgcc
      661 ggtcaagctg gcggagtacc atcgtgaggc gctggtgggg aaaaccatcc atacgagcgt
      721 caccctcaac aggcgggatg ctgtctggtg ggtaacgctc tcctacgacg aggtggttcc
      781 gttgcagacc cctccatcgg ctccggtcgt gggcatcgat gtgggcgttg ccaacttcct
      841 cacgacgagc gacggcaagc gctacggcac ctttcatggc aagctgcgcg agcgacagaa
      901 acgcgaccgc gagaaacggc ggcgcaaggc caaactgcgg gcatgtctcg aaaagaaagg
      961 cgtccaaaag ctgccctcga ccacgagccg tggcgcgcag cggctcgccc gccacgtccg
     1021 tcgggagatc aaccgggcgg tcaaccagtg cttcgccgca cacgagggcg cgcgggtcgc
     1081 ctatgaacac ctctcggtcg cgacgatgaa gcacaaggcg agagcgatga atgcctatct
     1141 gcgcgcgtcc aatctggcgc acctccccaa gcaaatcgaa tggactgcgg ccaaacgagg
     1201 gattctggca acccgtgtca agagcgccta ttcttctcaa gcatgcagtg tgtgctatta
     1261 tgtggacaga gccaaccgcc cgaaccagca aacgttttgc tgtcgggtct gcggctaccg
     1321 ggcgcatgcc gatgtgaatg cggccaccaa tattgagcgg cgtgtaggag acacagaact
     1381 acgcgcctgc aaggatcgga aagcggtcaa agccttactg atgcacaggc atcaggcgtg
     1441 gaagaagcaa aacggttggc cgtagtccaa ccgcccgccc agttcgtgcg tcaacgggcg
     1501 taggtcaagg ctagggtttg ggcagatgtc ctatttgtcc aggttttagt aactataaga
     1561 acgtttcacc gtgtttcaag taacgttagg ctttgcaaaa aaagcgcgca ctctcgcctt
     1621 gtttcccggt atacgcctat ggtacactat cgaaaagaag tacaaatgca atgcgataag
     1681 gtttctcatg gagcatcaat cggtcacgaa gcaagctgca aaggtgtccg gcagcgatga
     1741 acagagtcac gatagaaatc tgcttgggcc tctgctgtgc tgggcggttg tcttcgctga
     1801 cattggcact tcggtgtact atacgcccgg tattctctat cagacggtgg ggaagctggc
     1861 cggctttttc gtgctgctga ccatgaccgt ctttgttttg ctgacactga aatacgcaga
     1921 ggtgacgcac cgtttcccgc aaggcggcgg cgtcgtcacg gtagcagccc aggccatcaa
     1981 tcactgggtg ggcgcgctcg gcggtatgtt catcctcgtg gactatttcc tgacggcggc
     2041 catcagttgc ctgtcgggca tgatctatct cagcgtcgtc gtaccggcta ttggaccaat
     2101 cgctttgtgg atcacgatca tcgtgctggt gttgttgggc ctgctgaact gggtgggcat
     2161 cagtgagagc gcgaaggtga gcctggtggg cgccgttatc gccttcctca gcgatcttgt
     2221 catcctctac acggttttta cgcatatttc gctcggtcag tttttcgcgt tgttcggcga
     2281 gatgttccgg ggcaacccgc tgacgcccac ctcaatcctg gtcggcttcg caggctcctt
     2341 cctggcgttc tccggcctgg agagcatctc gcagctttcg ccggtgatgc gcacaccgcg
     2401 caagagggtc gccggcatcg cgctgttgct ggtcgtcctg accatcgggc taaccagtcc
     2461 actgctcacg atgctctcga ccctgctggt cacaaacagc gccggccatc cactcgcgac
     2521 cgacccggtg ctgtcggggc aactcatttc gttgttaggt ggcaaatggg gcggcgttat
     2581 tttggggacg gaagtggcga tcagcgccag cgctctgctc gtcttcgcca gcaataccgc
     2641 catcattggc gcgtaccacg tcttcatggc gctctcacgt atggagttct tcccggcggt
     2701 ggtgctaaaa cgtaataagc tgcgtggaac gccgcattat tcgatcgcgc tggcgacggg
     2761 cattccgatt atcattctca tcatcgtgcg gggcaatatt aatatcctgg gcgatatgta
     2821 cgcctttggc ctgctcggag catttacgct gacctgcctg ggattggaca tcgtgcgtca
     2881 ccgcgagcgc aggctggcgc gccggttgtc gaagcgcatt cgtcacgatc gactcaacgg
     2941 caggacggac ggtacagccg aaaagctggt ggagtcggct ctccatgaag tgaattcgtc
     3001 ggaccatcaa ccgtattcct cgaataatca cggccacgat cgcaacggcg tggaagagcc
     3061 aatcgtcggc gggattgagc taagcaaggt agcttcgctg gccgcgccgc gcaagtcatt
     3121 gcgcgcgtgg gtgcgtgatg cctggtacaa cctcgattac tggttaggtg tcatgacaac
     3181 ggcgctggtg ttcatcgcct ggtctacgaa cctggtgtcc aagccactcg caacggcctt
     3241 tggaggcaca gttgccacgc ttggcatggc tgtcgcctac tccaattatg cgcggcagag
     3301 acgcagggga cgcctgccgg tcatttcgat tggcgtcgag ggacgcctgc ccgattccat
     3361 cctggcggtg ctgactgctg gcaatggcca gaatgagatg gtgattcgtt cggccattaa
     3421 aagcgctgat ggcaagcccg tcatttttct gtacgtaggc gagccacaaa ccaatcgcag
     3481 accccagatg tttgaggtct ttgaccccta tcttgaggat gctcacgcca aagaatactt
     3541 cggcaaagcc gagcaactgg cgagtaaata taacgtcaac cggcgtttcc tctatcgcca
     3601 gcaggagcca ggcgttgtca gcgaggtatg gcaggtgatg cacccccacg acaccatcat
     3661 tgcggcgcag aacgtcggcg atgtcaagga agtcaacccc gaccgcattc gttatgagct
     3721 ggtacccggc ggcggcggca aggtggcgca cctgctgaaa cgctggtgaa gctagtgata
     3781 caataaagag accaacacgg acgtagtgag ctgttagccc gataaggaga aatcaatcaa
     3841 tgcaagaccg cattcgcgaa attttaagct ggtacaccag cgagaatcca ggcgtgcgta
     3901 ccaatctcgc tcgaatgctc aatcatggca ggctgggcgg cacaggaaag atggtgatat
     3961 tgccggtaga ccagggcttt gaacatgggc cggcgcgtag cttcgcccct aatcccgccg
     4021 ggtacgatcc gcgctatcac ttccagctcg ccatcgaagc tggctgcaat gcctacgccg
     4081 cgccgctggg tttcatcgag gcggctgttt ccgactactg cggcgaattt cccttgattt
     4141 tgaaatgcaa caaccacgat atcctgaacg atgagcgcga ccccatttct gccgtcactg
     4201 ccggcgtcaa tgatgccctg c
//
LOCUS       DASZAK010000701         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_123899, whole genome shotgun sequence.
ACCESSION   DASZAK010000701 DASZAK010000000
VERSION     DASZAK010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123899"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..1507
                     /locus_tag="VGT44_12370"
     CDS             347..1507
                     /locus_tag="VGT44_12370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2581639.1"
                     /translation="MSHLRVALTTTDDVYDTGGVENSIMRIARGLAASGVEVDVVMLR
                     LFERTVFQTGGSNGVTRLPSSVDGLRLYRITPWVGSEQAEQRWAEMHYSLLALARERG
                     YDLLQAFYASVAGFPTVYAAELLGIPSVVSIRGNDLIADVFHPQWFAQLVWALQHTTQ
                     LTAVSREGLERARVLCSSPAKGRVILNSVRPQDFLDGVQELALSRPVIGSLAVFRAKK
                     GMEVLLSAFRLLLERIPSAHLLLVGYVVPTEQRHFDELVAKYGLEGKFTLTGGIPHNE
                     SLRYLRAMDVFAFTSLHDGCPNAVLEAMLAGLPIVAASSGALPDMIEHGREGLLVQPG
                     SAMELCEAIERIVGDGEMGKAFGERARERVLRQFTLQRELREYVEVYRACGG"
     gene            complement(1658..2650)
                     /locus_tag="VGT44_12375"
     CDS             complement(1658..2650)
                     /locus_tag="VGT44_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEV2581640.1"
                     /translation="MNETSGETSSINRRVIQLRLRFNITRQNGSHAQLAPSEPVPLPP
                     PVALLIDGENILSSHLIPQMLEVASRLGNVTIRRVYGNWRNNPFDGHWRQAAIKYGLQ
                     PMHSIKGKETEKKESGKNGNDWEKHKNGSDISLVIGALDLLHYSSIRHFCIVTGDSDY
                     LPLITRLREAGCTIVGIGNPQTQQSLRLAYDSFLTTDDLIRSNTALQEPKPPANPILN
                     GNAEAAIDLPKPALAEPVTNHAEETLDQLLISAYQDCLENKKKYKDLPENEKWVYFPD
                     VGDSLQKLNKDYKKIYEIEFGWKNAYKYIKTRTDLFEWRVSKSPPQIDIKLRKR"
     gene            complement(2830..>3128)
                     /locus_tag="VGT44_12380"
     CDS             complement(2830..>3128)
                     /locus_tag="VGT44_12380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581641.1"
                     /translation="TLFLIALIVFIAILLMGIRHHWRWLFWLLLIAFGAMILDIPVTI
                     LQLMGIVPNLFPVWYSLCQMGVSLLAVLIATWMFSIYRRYGVWALGKQKISLHG"
BASE COUNT          654 a          776 c          931 g          767 t
ORIGIN      
        1 tacacgcagt ctggaggagg tcgtgcctgt cgtgcagcga ctcgcccgcg cggcgctcgc
       61 cgccgagaat gattattaac cactaagatt ggtaagtggt ctgaaagcga gcaagcctgt
      121 catcctgagc gcagcgaagg atctgaattc gtcaggcacc gagatcctga gcgcagcgaa
      181 ggatgacatt cccgatcttg gtggagaaag ttcattatcg gcctgtgaca ccgctatcga
      241 tgtgcaattc aggccgatca atcggcggtg agcgcgatac atcggctcct acagagaggc
      301 gagcatatcc cattgatgag ttaaagaatt tgagagaacc atagcaatga gccatttacg
      361 tgtcgccttg accaccaccg atgatgttta tgataccggc ggcgtcgaga actcaatcat
      421 gcgcatcgcg cgaggactgg ctgcctccgg cgtagaggtg gatgttgtga tgctgcgttt
      481 gttcgagcgg acggtgtttc agacaggggg cagcaatggc gttacgcggc tgccgtcgag
      541 cgtcgatggt ctgcgcctct accggataac gccctgggtg ggcagcgagc aggcggagca
      601 gcgctgggcg gagatgcatt atagcctgct tgcgctggcg cgcgaacgcg gctatgatct
      661 cttgcaggcg ttctatgctt ctgtggcggg tttcccaacg gtctatgccg ccgaactgct
      721 gggaattcca tcggttgtca gtattcgcgg caacgatctc atcgccgatg tctttcaccc
      781 acagtggttc gcgcagctcg tgtgggcttt gcaacatacc acgcagttga cggcggtcag
      841 ccgggaggga ctggaacgcg cgcgcgtcct atgcagcagc cctgcgaagg ggcgtgtgat
      901 cctcaacagt gttcgcccgc aggattttct ggatggcgta caggagctgg cgctgtcgcg
      961 tcccgtgatc ggctctctgg cggtcttccg cgccaaaaag ggcatggaag tgctgctctc
     1021 cgccttccgc ctgctgttgg agcgcatccc gtcggcgcac ctgctgctgg tcggctatgt
     1081 cgttcccacc gaacaacgcc atttcgatga actcgtggcg aagtatggtt tggagggcaa
     1141 attcacgctg accggaggca tcccgcacaa cgagtcgctg cgctacctgc gcgccatgga
     1201 tgtcttcgcc ttcacctctc tgcacgacgg ctgccccaac gccgtgcttg aagccatgct
     1261 agctggtctc cccatcgtcg ccgccagttc cggcgcgctg cccgatatga tcgagcatgg
     1321 cagggagggc ctgctggtgc agccgggatc ggcgatggaa ttgtgcgagg caatcgagcg
     1381 aatagtgggc gatggagaga tgggaaaggc gtttggcgag cgcgccaggg agcgtgtgtt
     1441 gcggcagttc acgctgcagc gggagctaag ggagtatgtg gaggtgtata gggcatgtgg
     1501 gggataattc gtggcggagt tatcgagtca ggagttgtca tcctgagcgc agcgaaggat
     1561 ctcggtcctc agacagtgag atccttcgct gcgctcagga tgacagactt gatcgtccct
     1621 ccagaccctc ttcctgatct tggtggtgaa ccttcattta gcgcttgcgc agcttgatat
     1681 cgatttgggg aggcgatttg gagactcgcc actcgaagag gtcggtgcga gttttgatat
     1741 atttgtacgc attcttccag ccaaattcga tctcgtaaat tttcttatag tccttgttca
     1801 gcttttggag gctgtcgccg acatccggga aatagaccca tttttcattt tctggcaggt
     1861 ctttgtactt ttttttgttt tcgagacagt cttgataagc gcttataagc agttggtcca
     1921 gagtttcttc ggcatgattg gtcactggtt cggccagtgc gggttttggc aggtcgatcg
     1981 cggcctcggc gttgccgttt aagatgggat ttgccggggg tttgggttct tgcagggcgg
     2041 tattgctcct gataaggtca tcagtggtga gaaaactgtc ataagcgaga cgcagcgatt
     2101 gctgggtttg gggattgccg attcctacta tggtacaccc ggcctcgcga aggcgcgtta
     2161 tcaggggaag gtaatcgctg tctcctgtga caatgcagaa atgtctgatg ctagagtagt
     2221 gcagcaaatc cagtgcgcct attaccaggg agatatccga gccattcttg tgtttctccc
     2281 agtcattacc attcttgcca ctttccttct tctcggtctc tttccctttg atgctatgca
     2341 taggctgtag accatattta atagcagcct gccgccaatg gccgtcgaag gggttattgc
     2401 gccagttgcc gtagaccctg cggatggtga cattgccaag tcgggatgca acctcgagca
     2461 tttgagggat caggtgcgag gagagaatgt tttccccgtc gattaatagc gcgacgggag
     2521 gtggcaaagg cactggctct gatggagcga gttgtgcatg agacccgttt tgacgggtaa
     2581 tgttgaagcg caaccgaagt tgaatgacac gtcgattgat gcttgaggtt tcgcccgatg
     2641 tttcgttcat tgccgtattt tccattcatt atatacgata tctgatcgat ggttagtgag
     2701 atatgcggaa attactacaa gtataaacgg atgtatcaat ggaaacaagt ggttgtgact
     2761 ttcttctcac gagtaacgct cgctatcgcc ctggttcatt tatgtgcatg tgtggttgct
     2821 tcgcacgtct tatccgtgca gagatatttt ctgcttgccc agcgcccaca ctccatagcg
     2881 ccgatagatc gagaacatcc atgtagcaat gagtaccgca agcaaggaaa cgcccatctg
     2941 acaaagactg taccagaccg gaaaaagatt aggaacaata cccatgagtt gtaagatcgt
     3001 cacaggaatg tccaggatca tcgctccaaa ggcgatcaac agcaaccaga agagccatcg
     3061 ccaatggtgt cggataccca tgagcaagat ggcgataaac actatcaggg ctatcagaaa
     3121 gagggtcg
//
LOCUS       DASZAK010000702         1737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_623128, whole genome shotgun sequence.
ACCESSION   DASZAK010000702 DASZAK010000000
VERSION     DASZAK010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623128"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..802
                     /gene="dhaL"
                     /locus_tag="VGT44_12385"
     CDS             173..802
                     /gene="dhaL"
                     /locus_tag="VGT44_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEV2581642.1"
                     /translation="MPIATEDVVRWIQQVASVLHENREYLTQLDSPIGDADHGINMDR
                     GFKAVLEKLPTVASTDIGSILKTVGTTLVSTVGGASGPLYGTAFLRMGMSTAGKNELY
                     EADVVAMLEAALEGIKARGKAQSGEKTMVDALTPALAAAKAAEAHNLGLSAMLRGAAD
                     AAEQGMKATIPMLATKGRASYLGERSIGHQDPGSTSSWLMLKALADTCG"
     gene            817..1227
                     /gene="dhaM"
                     /locus_tag="VGT44_12390"
     CDS             817..1227
                     /gene="dhaM"
                     /locus_tag="VGT44_12390"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HEV2581643.1"
                     /translation="MSVGLVIVSHSARLAEGVAELAGQMAQGRVSIAAAGGTAEGELG
                     TSVDKIVDALRQVDSPDGILVLLDLGSAVMATEMAVEAFAAISPHPVTISSASLVEGA
                     VIAAVEASIGNNLSEVAEAAVSAATLPKVTARHT"
     gene            1256..1531
                     /locus_tag="VGT44_12395"
     CDS             1256..1531
                     /locus_tag="VGT44_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEV2581644.1"
                     /translation="MPVAETRIVITNKVGLHARPARLLVQTAAQFHAQIQVSCGEKAA
                     NAKSIVGILKLAASLGDTLQLRAEGDDAEQAIQTLTDLIERKFDEEE"
     gene            1540..>1737
                     /locus_tag="VGT44_12400"
     CDS             1540..>1737
                     /locus_tag="VGT44_12400"
                     /inference="COORDINATES: protein motif:HMM:NF017346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HEV2581645.1"
                     /translation="MIDERAQAELSSLLSMPVMPISPGIAVGPARIIKAAQAASTLSE
                     ALIASSQVEAEQQRLYAAIAQA"
BASE COUNT          373 a          529 c          524 g          311 t
ORIGIN      
        1 ataaaggtgc gcccctacag acggatggta acattcaggg gcgattcatc gtacaatact
       61 gtcgagccgg gaaggggacg ccagcccctg gcgaaacatg ccggtacaat gaatggcccg
      121 cgaggaaagg cgaccaacac gcggtgtaca tctaccattt gaggagggaa ttatgccaat
      181 tgcaacagag gacgtggtgc gctggattca gcaggtggcc tcggtgctgc acgaaaatcg
      241 cgagtatctg actcaactcg actctcccat cggcgacgcc gatcacggca tcaacatgga
      301 tcgcggcttc aaggccgtgc ttgagaagct gccgaccgtg gcgtctaccg atattggttc
      361 gatcctcaag acagtcggca cgacgctggt ctccaccgtt ggcggcgcga gcggcccgct
      421 gtatggtacg gcctttctgc gcatgggcat gtccaccgcc ggcaagaatg aattgtacga
      481 agccgacgtg gtcgccatgt tggaggctgc cctggaaggc atcaaggcgc gcggtaaagc
      541 ccagtccggc gaaaagacga tggtggacgc gctgacgccc gcgctggccg ccgcgaaagc
      601 agctgaggca cacaacctcg gcctctccgc catgttgcgc ggcgccgccg atgctgccga
      661 acagggcatg aaggccacca ttcccatgct cgccaccaaa gggcgcgcca gctatctcgg
      721 tgagcgcagc atcggacacc aggaccccgg ttcgacctcc agctggctga tgctcaaagc
      781 cttagctgat acctgtggat aaggagatag agactcatgt ctgttggctt agtgatcgtg
      841 tcgcacagcg cgcgcctggc cgaaggcgtc gccgaactgg cgggccagat ggcacaggga
      901 agggttagca tcgcggcggc aggtggcact gcggaggggg aacttggcac ttcggtcgat
      961 aagatcgtcg atgcgctgcg ccaggtcgat agccccgatg gcatcctggt gctgctcgac
     1021 ctgggaagcg cggtgatggc caccgaaatg gccgtcgaag cctttgccgc gatttcgccg
     1081 caccccgtca cgatttcctc agcctcactg gtcgaaggcg cggtcatcgc cgcagtcgag
     1141 gcgtccatcg gcaacaacct gtcggaagtg gctgaagccg ccgtcagcgc cgctacgctg
     1201 cccaaagtaa ccgctcgcca cacgtgatac cctcatacac aaaaggacaa ctattgtgcc
     1261 agtagctgaa accaggatcg tcatcactaa taaagttggc ttacatgctc gtcccgcgcg
     1321 cttgctcgtc cagaccgccg cacaattcca cgcgcagatt caagtatcct gcggcgagaa
     1381 ggccgccaat gccaaaagta tcgttggtat tctcaagctc gctgcctccc tgggcgatac
     1441 attgcaattg cgcgccgaag gcgacgatgc cgagcaggcc attcagacgc taaccgacct
     1501 gatcgagcgc aagtttgacg aagaggagta agccagccga tgattgacga gcgcgcgcaa
     1561 gcagaactgt cctcattgct ttctatgccg gttatgccga tttcacctgg catcgctgtc
     1621 ggcccggcgc gcatcatcaa agccgctcaa gcggcctcaa cgctctctga ggcgctgatt
     1681 gcgtccagcc aggtagaagc tgagcaacag cggctttacg cggccatcgc gcaggcc
//
LOCUS       DASZAK010000703         2824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_267406, whole genome shotgun sequence.
ACCESSION   DASZAK010000703 DASZAK010000000
VERSION     DASZAK010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267406"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VGT44_12405"
     CDS             complement(<1..566)
                     /locus_tag="VGT44_12405"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV2581646.1"
                     /translation="MKKRYYWFTGVGLILIIAVAGLMYTAHFASAVSQTEAHVAAGAA
                     TATGTPLPTKGTSTFTIVSTGTTASYSVYENLVFQNKPNNDAIGTTHSVTGSFSIHSG
                     TSPLVGNMTIKVDLRTLQSDSSRRDQFIQQNTLQTDSYPYATFVSVSTSGLPASYTDG
                     QTVKFQITGNLTMHGVTNKEVFTVQGKVV"
     gene            834..2018
                     /locus_tag="VGT44_12410"
     CDS             834..2018
                     /locus_tag="VGT44_12410"
                     /inference="COORDINATES: protein motif:HMM:NF006091.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HEV2581647.1"
                     /translation="MDIRTPVGIIGAGPAGLFLSLLLSLEGIDSIVLESRSREDIEST
                     IRAGVLEQGTVDLMVEMGIGERMKREGFVHHGIELRFDGEGHRIPLSELANGRAITLY
                     PQHEVIKDCVAARLDQGGRILFGVKDVQLSDVAGDQPVIRFIHEDDLVELQCDFIAGC
                     DGFHGVSRASIPADIQRVFSRTYPFGWLGILMQAPPSAEELIYAYHEQGFALVSTRSP
                     DLQRMYIQCDPRDDIANWSDAMILDELQARLETGDNWTLRLGPIIQKSILAMRSFVIE
                     PMQYGRLFLAGDAAHIVPPTGAKGLNLAVADVRHLARAITLFYQQRQAHLLEAYSATC
                     LRRVWRTEHFSWWMTSMLHRFPDDDAFQQRMQRAQLDYTVRSRAASLSLAENYVGLPF
                     DW"
     gene            complement(2042..2245)
                     /locus_tag="VGT44_12415"
     CDS             complement(2042..2245)
                     /locus_tag="VGT44_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581648.1"
                     /translation="MIINHTDEQQWVLEALDKSQLVEEKQNKRTGLRHFGRGMVALMW
                     LLRLYVVFMLLLVAHQAWTIVVR"
     gene            complement(2316..>2824)
                     /locus_tag="VGT44_12420"
     CDS             complement(2316..>2824)
                     /locus_tag="VGT44_12420"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV2581649.1"
                     /translation="LFIHHISAANFATADFAQALAPLIGHVGASLFALGIVEAGLLAT
                     ITITASSAYAFGEVTATPHSLNRPLREAWPFYVTMLLAAGMATALVLWPQAPLELIVL
                     IVNVIATLSMPPALLFLFLLVNDREIMGAHINSRLTNIVVIAIVVFLCLAGLTFGVVS
                     VFPSILPS"
BASE COUNT          633 a          803 c          750 g          638 t
ORIGIN      
        1 accaccttgc cctgtaccgt aaacacctct ttgttggtga cgccgtgcat ggtcaaatta
       61 ccggtgatct gaaatttgac cgtttgacca tcagtataac tcgcgggtag acccgacgtg
      121 ctgacggaga cgaatgtggc atagggatag gagtctgttt gcaacgtgtt ttgctgaata
      181 aattgatcgc gcctcgaaga atccgattgg agcgttctca gatcgacctt gattgtcata
      241 ttgccgacca ggggcgatgt cccgctatga atactgaaac tgcccgtgac cgagtgcgtt
      301 gtgccaatgg catcgttgtt cggtttgttc tggaagacca ggttttcgta gacgctgtaa
      361 gaagcggtcg tcccggtaga tacgatggta aaagtggacg ttcctttcgt tggcagaggc
      421 gtccctgtag cagttgcggc tccagccgcg acgtgcgctt ccgtctgact gacagcgctg
      481 gcgaaatgcg ccgtgtacat cagaccggca acagcaataa tgagaattaa cccgactccc
      541 gtaaaccagt aatagcgttt tttcatggtt cctcctgtaa tatccaatga atctgtagtt
      601 ttcaattagt gaagcaggag gcatatcgcc atcgacaagt gattcttgaa cctttagaaa
      661 tcctctcctc cccgtttcgt tcacttctcc tttagtctag cagtatacca ttgaaatctt
      721 attggagttt aatgagaaat ttctcatctt gcaacagtca gtataaacac gatagaattg
      781 gtacaactat agagccatct aaagcaagca cgtccgtaag gagaactacg caaatggaca
      841 tcagaacgcc cgtcggaatc atcggggccg gaccggcagg gttgttcctg tcgctgctcc
      901 tctccctgga aggcattgat tcaatcgtgc tggaaagccg cagcagggaa gacatcgaat
      961 cgacgatccg cgctggtgtg ctggaacagg gaacggtcga tctaatggtg gaaatgggca
     1021 tcggcgaacg catgaagcgc gagggctttg ttcatcatgg catcgaactt cgttttgacg
     1081 gtgaggggca tcgcatcccc ctctcggaac tcgccaacgg gcgtgccatc acgctctatc
     1141 cccagcacga agtcatcaag gattgtgtgg cggcgcggct cgaccagggt gggcgtatcc
     1201 tgttcggcgt caaggacgtt cagctctccg atgttgctgg cgaccagccg gtgatacgct
     1261 ttatccacga ggatgacctg gtcgaattac agtgcgattt cattgcgggc tgcgacggtt
     1321 tccacggcgt gtcgcgcgcg tcaattccag cagatatcca gcgtgtcttc agccgcacct
     1381 acccctttgg ctggcttggt atcctgatgc aggcgcctcc gtccgccgag gagttgattt
     1441 atgcctacca cgaacagggt tttgccctgg tcagcacgcg ctcgccggac ctgcagcgca
     1501 tgtatatcca gtgcgacccg cgcgacgata tcgccaactg gtctgacgcg atgatcctgg
     1561 acgaactgca ggcacgcctg gagaccggcg acaactggac cctgcgcctg gggccgatca
     1621 ttcagaagag catcctggca atgcgcagct tcgtgatcga gccgatgcaa tatggacggc
     1681 tctttctggc gggagatgcc gcgcatatcg tgcctcccac cggggcgaaa ggactgaacc
     1741 tggccgtcgc cgatgtccgg catctcgccc gcgctatcac gctcttctac caacagcgcc
     1801 aggctcatct cctcgaagcc tattctgcca cctgcctgcg gcgcgtctgg cgcacagaac
     1861 acttctcctg gtggatgacc tccatgctgc accgtttccc agacgacgat gcctttcaac
     1921 agcgcatgca gcgcgcccag ctagactaca ccgtccgctc gcgtgcggcc tccctcagcc
     1981 tcgccgaaaa ctacgtcggc ctcccgttcg actggtaaag cgcgctgcca ttactactgt
     2041 ttcacctgac aacgattgtc caggcctggt gagccacaag cagcagcatg aagacaacgt
     2101 aaagacgtag aagccacatc agcgccacca tgccacgccc aaagtgacgc agacctgtgc
     2161 gtttgttctg tttttcttcg acgagctggc ttttatccag cgcctctagc acccattgtt
     2221 gttcgtctgt gtgattgata atcattatta tttcctccct catttttctt aatttggcct
     2281 tctgatacta cacatgtatg aatgagtgcc tccctttacg acggcaaaat cgaagggaac
     2341 acactgacaa ctccaaaggt cagccctgcc aggcagagaa acactacgat ggcaatcacg
     2401 acaatatttg tcaatcggct gttgatatgc gctcccataa tctcccggtc attgaccagc
     2461 aggaagagaa acaacagcgc aggtggcatc gacagtgtgg caatcacgtt cacgattaag
     2521 acaatcaact caagcggcgc ctggggccac agcacaagag cggttgccat gccagccgcc
     2581 agcagcatcg taacatagaa cggccaggct tcgcgcagcg gtcgattcaa gctgtgcggc
     2641 gtggctgtca cctcaccgaa agcataggcc gacgaagcgg taatcgtgat cgttgcgagc
     2701 aggcccgcct ccacgatgcc tagcgcgaac agcgacgccc cgacgtgccc aatcagtggc
     2761 gcgagggcct gcgcgaaatc agccgtcgcg aaattcgctg cgctgatgtg atggataaac
     2821 agtg
//
LOCUS       DASZAK010000704         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_549898, whole genome shotgun sequence.
ACCESSION   DASZAK010000704 DASZAK010000000
VERSION     DASZAK010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549898"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1977
                     /locus_tag="VGT44_12425"
     CDS             <1..1977
                     /locus_tag="VGT44_12425"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEV2581650.1"
                     /translation="GEALYGLGSHEEGFLNLRGTRQDLYQENLKAAVPMLVSTNGFGI
                     LLDCYSLMTFHDDEQGSFLWCDIQDELDFYFIYGPEFDQVVQGFRFLTGKVPMLPRWV
                     FGYLQSKERYTSQEELLAVVKEYRRRQIPLDGIVLDWHSWPGDLWGQKSLDPERFPQP
                     QAMIEELHRLQVRLMVSIWPTMQNHGPNHLEMLEHGFLLGDSVTYNAFDARARALYWK
                     QANEGLFSLGVDAWWCDCTEPFEADWTGETKPAPQERRRINTEEAKKYLDPQYINAYS
                     LQHSRGMYEGQRLTTSSKRVVNLTRSAYAGQQRYGTITWSGDISAKWQTLKQQIAAGI
                     NFCITGLPYWTADIGAFFVDKKELWFWDGDFPEGCEDLGYREFYVRFFQYGTFLPLFR
                     SHGTDTPREVWRFGEPGSRFYETLVTFIRLRYRLLPYIYSLAGRVTQDDYTMLRALAF
                     DFRQDSQVYNIADQYLFGPAFLVNPVTEPMYYLPGSQPLPPHSQTRQVYLPAGSDWYD
                     FWTGRRFSGAQTVKADAPLEILPLFVRAGSIVPFGPDIQSSAEARRNSALLLRIYQGQ
                     DATFTLYEDDGDTYNYENGTFSTIALHWDDAKKRLYIGRRQGAYPGMPLTRTFSLLVA
                     SEQQGTALDSSSEGAREITYDGNPLVVEMNGEPE"
     gene            complement(2157..>3062)
                     /locus_tag="VGT44_12430"
     CDS             complement(2157..>3062)
                     /locus_tag="VGT44_12430"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEV2581651.1"
                     /translation="TQTLTVSVSNLSAQVCDESIALDAPEFGVSPDTLNPTLTIPPQQ
                     ARSFVWILIPQKLGTFALAISFSSPEIAQTIGITVTNVLGLTIWQAQLLSDGGTLLGV
                     FFGPLLAIVWMSRVSWRRKKARGKWTQHMLPKKRFLPANGGNPLSLIYCFTHEDKALR
                     DRLEQHLSPLLRSGHISTWYDDEITPGKEWEDEIMAHLNAADIILLLISPDFVACDHC
                     MSQQMTRAMERHHAGEATVVPILLRHVDWESLPFAKLRVLPLDRRPVSASDWTTEDEA
                     FYDVVQGIRQVVGSLLAVKARSSQA"
BASE COUNT          726 a          797 c          839 g          700 t
ORIGIN      
        1 ggggaagcgc tgtacggcct ggggtcacat gaagaaggct tcttgaatct gcggggaaca
       61 cgccaggatc tctaccagga aaatttgaaa gccgcggttc cgatgctcgt ctcgaccaac
      121 ggctttggta tcttgctgga ctgttattcc ctgatgactt ttcatgacga cgagcagggg
      181 tctttcctgt ggtgcgatat ccaggacgaa ctcgattttt attttattta cggtcctgaa
      241 tttgatcagg ttgtccaggg cttccgcttc ctgaccggca aggtccccat gttgccgcga
      301 tgggtctttg gctatctcca atcgaaagaa cgctatactt cgcaggaaga actcctcgcg
      361 gtggtcaaag agtaccgacg acgccagatc cctcttgacg ggattgtact cgactggcat
      421 tcctggccgg gagacctctg ggggcaaaaa tccctcgatc cagaacgttt tccccagccg
      481 caagccatga tagaagaact gcaccggcta caggtcaggc tgatggtttc catctggccc
      541 acgatgcaga atcatggccc caaccatctg gagatgctcg aacacggctt tctgctagga
      601 gattcggtga cgtataacgc ctttgatgcg cgggccaggg cgctctactg gaagcaggcg
      661 aacgaaggac tcttctccct gggagtggat gcctggtggt gcgattgtac cgaacccttt
      721 gaagcagatt ggacgggaga gaccaagcct gcaccacagg aacgacgaag aattaataca
      781 gaagaggcaa agaagtacct cgacccccag tatatcaacg cctactcgct gcagcacagc
      841 cgaggaatgt atgagggtca acgcctgacc accagcagca aacgggtcgt caatctgacg
      901 cgttccgcat acgcggggca gcaacgctat gggaccatca cctggtcagg tgatatttcg
      961 gcgaagtggc agacgctcaa acagcaaatc gcggcaggca ttaatttttg cataaccgga
     1021 ttgccctact ggacagcaga tattggagcc ttttttgtcg acaaaaaaga gctgtggttc
     1081 tgggacgggg actttccaga gggatgcgag gacctcggct accgcgaatt ttatgtgcgc
     1141 tttttccagt atggaacctt tcttccgctc ttccggtctc atggaactga cactccgcgt
     1201 gaagtgtggc gctttgggga gccgggttcg cgtttttatg agacgctggt gacgttcatc
     1261 cgcttacgct accggctctt gccctatatt tactcactgg ccggtcgcgt aacgcaggac
     1321 gattatacca tgctgcgcgc cctggctttc gatttccggc aggattccca ggtctataac
     1381 attgccgatc aatacctctt cgggccggcg tttctcgtga atccggtcac ggagccgatg
     1441 tactaccttc ctgggtcgca accactccct ccacattccc agacacgcca ggtctatctg
     1501 ccagcaggca gtgattggta tgatttctgg acagggagac gcttttcagg agcgcaaacg
     1561 gtcaaagcgg atgctccatt ggaaattctt cccctgttcg tccgggcggg atcaattgta
     1621 ccctttggcc cggatatcca atcgagcgcg gaagccagga ggaactccgc tctcctgctg
     1681 cgtatctatc aggggcaaga tgccaccttt acgctctatg aggatgacgg ggatacatat
     1741 aactacgaaa acggaacctt ttcaacgata gcattgcact gggatgatgc aaagaaacgc
     1801 ctgtatatcg gaagaaggca gggagcctat ccaggcatgc ccctgacgag aacgttttct
     1861 cttctcgtgg cctctgagca acaggggaca gccctggata gctccagtga gggggccaga
     1921 gaaatcacct acgatggcaa cccactggtc gttgagatga acggggaacc agaataagga
     1981 actggctaag tttttcagca tcgcttggct cactatcgcg agtctctata aaagcgggtg
     2041 aagctcatcc gtattgagaa gaattaatca gatggctaaa ggcagccaag ccaatagaag
     2101 aaaaggggag gaattatcca ccaccgagag gggctatacc cgccggtctc tatcactcag
     2161 gcctggcttg acctggcctt gacggcaagg aggcttccaa caacctggcg aatgccctgg
     2221 accacatcat agaaggcttc atcttccgtt gtccaatcag aggcgctcac aggtcttcta
     2281 tctaagggaa gcactcgcag ttttgcaaag ggcaggctct cccagtctac gtgacggaga
     2341 agaatgggaa cgacagtagc ctcaccggca tggtgtcgct ccattgcccg tgtcatctgc
     2401 tggctcatgc aatggtcgca tgcgacaaag tccgggctaa tcagtagcaa gatgatatcg
     2461 gcagcgttga ggtgggccat gatttcatct tcccactcct ttccgggagt gatttcgtca
     2521 tcataccagg tgctaatgtg gccagaacgc aggagagggc taaggtgttg ctctaatcgg
     2581 tcgcgcaagg ctttatcttc atgggtaaag caatagatga ggctcaaggg gttgcctcca
     2641 tttgctggta gaaatctctt ctttggtagc atgtgctgag tccattttcc tctggccttc
     2701 tttctgcgcc acgaaaccct actcatccac acaatggcca gtagtggccc aaagaacacc
     2761 ccaagcaggg ttccgccatc agaaagcaac tgtgcttgcc agatcgtcag gccgaggacg
     2821 ttggtcacgg tgatgccaat cgtttgggca atctcgggag atgagaaaga aatggcgagc
     2881 gcgaatgtcc ccagcttttg cgggatcaaa atccatacga aactgcgtgc ttgttgggga
     2941 ggaatggtta gcgtaggatt caaagtatct ggactgaccc caaattcagg agcatccaga
     3001 gcaatggact cgtcacaaac ctgcgcactg agattggaga cagagacggt taaagtctgt
     3061 gt
//
LOCUS       DASZAK010000705         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_89581, whole genome shotgun sequence.
ACCESSION   DASZAK010000705 DASZAK010000000
VERSION     DASZAK010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89581"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..2664
                     /locus_tag="VGT44_12435"
     CDS             340..2664
                     /locus_tag="VGT44_12435"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV2581652.1"
                     /translation="MAQTVEQTSSHTSIGHSTRRQDGFDKVAGNTRYAGDLPLVGLLH
                     ARLVLSPYAHARILRIDTDAALRIPGVKAVYTSKTLGIVHPDNNDRSRSPLAEEEVLW
                     CGHPVAIVLAESEEAARDGVDVVDVEYEELPVVLDPLAAMQPGAPLTRLYVKETASEI
                     AGGGAHAAVSKEEVAEVDTETLSENVSDKAHMHEGDMEAGWREAEVVVERTYYTSSVH
                     QSYIEPQSITVAPGISKQHLLVWPSSQGMFGVRSSVAQTLDLPERQVRVESTPIGGAF
                     GGKFGLLEPLAAAAAYTSGKPVRLVLSRSDDLFAGNPAPQSMITLKLGAKRDGTLVAM
                     QAKVVFNTGAYGGSAVFLGGLLLGSSYRCPNFDFRCYEVMTNTVGVGAYRAPAAPQAT
                     FALESAVDELCHALGMDPVEFRLKNGVKEGDPSLMSKTWPRIGLLECLEKAQAHPLWA
                     QRAAHSAAGPAELNGWKIGVGIAAGGWPGGTEPTAAACRLESDGTVSIVVGTVDLTGS
                     DTSLALIASEALGVSADAVRTAHDNTDTMPYSGGTGGSKTTYSMGAAVLAAARDARSQ
                     ILSIASEMLEAATDDLDIEGDKVIVRGAPGKSVELKQIAGAAMQFAGRYEPVYGRGRA
                     ALKVSSPMFAVHVARVAVDPETGIVRVLDYVAAQDVGFAINPAEVEGQISGGVTQGLG
                     WALYEGYVYDENGQLLTSNFMDYALPHTEDVPHIATLLVEIPSALGPYGAKGVGEPPV
                     VPVSAAIANAIYDAVGARVTRLPITPERLFMAMQ"
BASE COUNT          544 a          856 c          899 g          554 t
ORIGIN      
        1 gtcttctcgg aaagtcgctg attgggcagc atcatgtgat aggccagtcc aaaaatactg
       61 gccacgctca ggacgcacaa taaaagtatg atggtcttcg atggtcgtga atgcgcaact
      121 cgccgcatct tttcccccag attggtgatt gctccatggt gtgtgacgca tagaccgatc
      181 actatgtatc gcgtgtggta cttctttcgc ccgcgcgggc atcacaattt tgtgctacac
      241 tttacggtga aaagcgggct tgtctcgcgt acattctctg gatcatcgcg tcccatgacc
      301 agggtggtcg ccgtcaaata gacaggagca atatctttca tggcacaaac agttgagcaa
      361 acatcgtctc atacatccat cggccactcg acgaggcggc aggatggttt cgataaggtg
      421 gctggcaata cacgctacgc gggtgacttg ccgctggtag ggctgctcca tgcccgcctg
      481 gttctcagcc cgtatgctca cgcgcgcatt cttcgcatcg atacggatgc cgcgctgcgc
      541 attccaggcg tcaaggccgt ttacaccagc aagacgctgg gcatcgttca ccccgataac
      601 aatgaccgct cgcgttctcc cctggcggag gaggaggtct tgtggtgcgg gcatcccgtc
      661 gcgattgtgc tggccgagag cgaggaggcc gccagggatg gcgttgatgt tgtcgatgta
      721 gagtacgaag aactgccggt cgtgcttgat cccctggcag ctatgcagcc gggcgcgcca
      781 ctgacgcgcc tctacgtcaa agagacggcc tcggaaattg cgggaggcgg cgctcacgcg
      841 gcggtctcca aagaagaagt ggcggaagtc gataccgaga cgctatccga gaatgtcagc
      901 gacaaggcgc atatgcacga gggtgatatg gaagctggct ggcgcgaagc cgaggtcgtg
      961 gttgagcgca cttattatac cagcagcgtg catcaaagct acattgagcc gcagtccatc
     1021 acggttgcac caggcatctc gaagcagcac ctgctggtct ggcccagctc gcagggcatg
     1081 ttcggtgtgc gttccagcgt tgcgcagacg ctcgacttgc cggagcgaca ggtgcgcgtg
     1141 gaatcgaccc ccattggcgg ggcattcggc ggtaagtttg gcctgctcga accgctggcc
     1201 gccgccgccg cgtatacctc tggcaaaccg gtgcgcctgg tgctgtcgcg ctcggatgat
     1261 cttttcgcgg gcaatcccgc gccgcagtcg atgattaccc tgaaattggg ggcgaagcgc
     1321 gacggcacac tggtggcgat gcaggcgaaa gtcgtcttca atactggagc gtatggcggc
     1381 tcagccgtct tcctgggcgg cctgctgctt ggcagctcct atcgctgccc caatttcgac
     1441 tttcgctgct acgaggtaat gacgaatacc gtcggcgtag gagcctatcg cgcgcctgcc
     1501 gcgccccagg ccaccttcgc gctcgaatca gccgtcgatg aactctgcca tgcgctgggc
     1561 atggacccgg tcgagtttcg cctcaaaaat ggcgtcaagg agggcgatcc ctcgctcatg
     1621 agcaagacct ggccgcgcat cgggctactg gaatgcctgg aaaaagcgca ggcgcacccg
     1681 ctctgggcgc agcgagcggc gcacagtgcc gccgggccag ccgagttgaa cggctggaag
     1741 attggtgtgg gcatcgcggc tggcggctgg cccggcggca cggagcctac cgctgctgcc
     1801 tgtcgcctgg aaagcgacgg caccgtctcg atcgtcgtcg gcactgtcga tctgaccggc
     1861 tctgatacgt cgctggcgct catcgcttcc gaggcgctgg gggtcagcgc cgatgccgtg
     1921 cgcaccgcgc atgacaacac cgatacgatg ccctacagcg gcggcaccgg cggcagcaag
     1981 acgacttatt cgatgggcgc ggcggtcctg gcggcagccc gcgacgcgcg cagccagatc
     2041 ctctccattg cctccgaaat gctggaggcg gcgactgatg acctggatat cgagggggat
     2101 aaagttatcg tgcgcggcgc gccgggcaag agcgttgaac tcaagcagat cgctggagcc
     2161 gccatgcagt tcgcaggccg ctacgagccg gtctatggac gcgggcgcgc cgctctcaaa
     2221 gtcagctcgc ccatgttcgc cgttcacgtg gcgcgcgtgg ccgtcgatcc agaaacgggc
     2281 atagtgcgcg tactggacta cgtggccgcg caggatgtcg gcttcgccat caacccggcg
     2341 gaggtagaag ggcagatcag cggcggcgtc acacagggcc ttggctgggc gctctacgag
     2401 ggctatgtct acgacgaaaa cggccagttg ctcacctcga acttcatgga ctacgccctg
     2461 ccgcatactg aagatgtgcc gcacatcgct accctgctag tggagatccc gtccgcgctc
     2521 ggaccctatg gcgcgaaggg cgtgggcgag ccacccgtcg tcccggtcag cgcggccatt
     2581 gccaacgcca tctacgacgc cgtcggcgcg cgcgtgacgc gcctgccgat tacgccggaa
     2641 cggttgttta tggcaatgca ataggggaaa cgggcatgtg ctatactgta tcagcgctac
     2701 cctcacatca cgcatagctc ttgtgtgacg tgtccgtaat gtgttttttc aatgtgtaga
     2761 gaacaccgac ggccaccggt tacgctatct ggcggttaca cctcataacg tagcccctga
     2821 ccagtctagg gagcaagcga atgatgaaac ctg
//
LOCUS       DASZAK010000706         5445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_89599, whole genome shotgun sequence.
ACCESSION   DASZAK010000706 DASZAK010000000
VERSION     DASZAK010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5445
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89599"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VGT44_12440"
     CDS             complement(<1..276)
                     /locus_tag="VGT44_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581653.1"
                     /translation="MIDPRPDAQPSAQQGRTIPIQERSVRVGDVIVVTGYRVKRSSLT
                     ARFAEGGYQVHETRHFLLFTRTEEPKLILVHWFAPEDLHTNLSHYLAE"
     gene            complement(300..1664)
                     /locus_tag="VGT44_12445"
     CDS             complement(300..1664)
                     /locus_tag="VGT44_12445"
                     /inference="COORDINATES: protein motif:HMM:NF012677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV2581654.1"
                     /translation="MRDIHDPPTQLEVHDPDILANARAVLDGCRSPREMQEAVLAAVA
                     RNEEWRGRQCINLLAPEAPTSPTVRALLSSEVGIRAAEGHIGPVNRWFAGTQHIDEIE
                     ALCVELLKQVFHARYADHRLVASMIGNMAVYTALTEPGDVIMSLSQPFGGHSSNRSDG
                     PAGVRGLKIVDIPFDPIELEVDLDLFRRMAPLVRPKVVALGASMTLFPFPLQAMQEVI
                     AEWGGRLFFDGAHQLGLVAGGQFQDPLREGAAVMTGSAGKTFSGPQSGIIVWDDSELT
                     EPIMHAIFPVLAATHQVNRVAALAASAAELLAFGQVYMAQIVRNAQALGAALDRHGIP
                     MLGAYKGFTTTHQVIADVRRFGGGLHVAQQLARANIITNKNLIPADKPQNWDRPGGLR
                     LGTIEVTRLGMGEAEMETIADFIARILVERVEPEAVLDDVIAFRQPFQTLYYCFEHGL
                     PVQA"
     gene            complement(1821..3056)
                     /locus_tag="VGT44_12450"
     CDS             complement(1821..3056)
                     /locus_tag="VGT44_12450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2581655.1"
                     /translation="MDTIIRQAHLRGRESTWDIGITGDRIAAVAEHLPDEATTEVDAA
                     GNLALPTYVNGHIHLDKCFLQETMRPNKDYTFGECLELTWEHKARYTTEDILERAGRA
                     IREGLLNGTTVFRAFADVDSIGQLRGLEGLLALREQRRDQAHIEVVTFPQEGILRDPG
                     TAELLEEAMRLGADVVGGLPWFELLDEDARTHIDFCFALARRYNKDIHMLIDDTDNPL
                     SRSLEYLAVKTLREGYQGRVSASHCGALAAYNEVHAHKVMELLKQAEVSISSNPHISL
                     VVQGRYDQEPVRRGITRVKQLWQHGVNVFSSQDDVADPFYPFGRNDQQEVAAYLCHTA
                     HLAAPSEIEAAFDFVTYNAARALRLPDYGLEPGCKADLNVLAAPTLREVLRLQQPPRW
                     VLRGGKVLASTQVVRTLAP"
     gene            complement(3147..>5445)
                     /locus_tag="VGT44_12455"
     CDS             complement(3147..>5445)
                     /locus_tag="VGT44_12455"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEV2581656.1"
                     /translation="LNRSLRLLSVLGVLTFPAEERGEVVMRVNFLQEAASSDEIKIDL
                     TSLRQVERCEERKLALMQEYALSPAERRGQLLRSYFLGEQPLIEPFVPRQDLTDEQRA
                     IVRLSEGYHLIQGPAGSGKTTLLQEHFRYLVEYHLVPAERILVTAHFHSAVDRVGNQL
                     DYLQSGGRPIPTKTLQSISTGIFRQHVQLLKQVSGQPYFPEGKAIQLLTGTWQEVDER
                     ELALVSQALDRLYRANGMLEYLPAHMEPLRLLGIYRPNASTERDCLKAISLFRTLGIY
                     PASISDNEELLTTLKGIGNRNAWLLRKLAFYYATFIAYQFVQGEQGVYTYDDQVLFAL
                     VILRANPDIAKRWQGKYEHIIIDEFQDFTPAEAEMIGILSHTHRNVMAVGDMRQRILD
                     NPKEQVPLSATFELVGNDMPVQEHRLTTNFRSVQDILDFACAVLSSFQWEHQQSALGS
                     KGIKPTVIRIDPNTLFASEHERSTGSLIVAMVDAALYHMGSLPPEVAGSVAFLVARSD
                     LSYPVQQCLKRRGCLFAVLESQHRYQSYHARRVLTYFRLIQDSNQTNEMERLLRHCVS
                     PYFSTSQIARLNEIARRLGMKLLDAALEPGVCEQIGATEEHLAVLQQHVAIIRKHSPD
                     STCKAVWQSIQAMRDELANQSDAREQTDEYIQEKNEIEEELSKLEEKSVQEALEHIDS
                     YITFIEENQAHRKLVVATLNNAKSQDFDTVFLLGADALKARHRWYVSVTRARQRFYCL
                     VDASKDNEVLASVPKGLFEEVSWPE"
BASE COUNT         1061 a         1574 c         1580 g         1230 t
ORIGIN      
        1 ttctgccagg tagtgagaga ggttggtgtg gaggtcctcg ggcgcaaacc aatgcaccag
       61 gatcagcttc ggctcttctg tgcgagtgaa gagcaggaag tgtctggtct cgtgaacctg
      121 gtagccgcct tcggcgaaac gcgctgtgag cgagctgcgc ttgacgcggt agccagtcac
      181 cacaatcacg tcgccaacgc ggacgctccg ctcctggatc gggatcgttc tgccctgctg
      241 cgcagacggt tgagcgtctg gtcgcgggtc gatcatacag tcttccttcc cagagagtgt
      301 taggcctgga cgggtaatcc atgctcgaaa caatagtaga gtgtctggaa aggctgccgg
      361 aaagcgatca cgtcatcgag caccgcttcc ggctcgactc gctcaaccag gatgcgcgcg
      421 atgaagtcgg cgatggtctc catctcggcc tcgcccatgc ccaggcgcgt gacctcgatg
      481 gtgcccaggc gcaacccacc gggacgatcc cagttctgcg gtttgtcagc ggggatcagg
      541 ttcttgttgg tgatgatgtt ggcgcgcgcc agctgctgcg ccacgtgcag cccgccgccg
      601 aatcgccgca catcggcgat cacctggtgg gtggtggtga agcccttgta cgcaccgagc
      661 atgggaatgc cgtgccggtc aagcgccgcg ccgagcgcct gggcattgcg cacgatctgg
      721 gccatgtaga cctgcccgaa agcgagcagt tcagcagcag aagccgccag ggccgccacg
      781 cggttgacct ggtgggtggc tgctaagaca gggaagatgg catgcatgat gggttctgtt
      841 agctcggagt cgtcccagac gatgatgccg ctctgcggac cgctgaaggt cttgccggcc
      901 gatccggtca tgacggctgc accctcgcgc agcgggtcct ggaactggcc gcctgccacc
      961 aaccccaact ggtgcgcgcc atcgaagaag aggcgacctc cccactcggc gatcacctcc
     1021 tgcatggcct gcagcgggaa ggggaagagc gtcatggagg cgcccagcgc gacgactttg
     1081 gggcgcacga gcggggccat cctgcggaag aggtcgagat ccacttccag ttcgatggga
     1141 tcgaagggaa tatccacgat cttcaggccg cgcacccctg ccgggccgtc gctgcggttg
     1201 ctcgaatggc cgccgaaagg ctgggacagg ctcatgatca catcgcccgg ttcggtgagt
     1261 gcggtgtaga cggccatgtt gccgatcatg ctggccacca agcgatggtc cgcgtacctg
     1321 gcatgaaaga cttgcttcag caattcgacg cacagggctt cgatctcgtc gatgtgttgg
     1381 gtgccggcga accagcggtt gaccgggccg atgtgtcctt cggctgcgcg aatgcccacc
     1441 tctgaagaaa gcagcgcacg caccgtcggg ctagtgggtg cctcaggggc gagcaggtta
     1501 atgcactgcc tgcctcgcca ttcctcattg cgcgccaccg ccgccagcac tgcctcctgc
     1561 atctcccgtg gagagcggca gccatccagg acggccctgg cgttggccag gatgtcgggg
     1621 tcgtgcactt ctagctgtgt aggtgggtca tgaatatcgc gcatgggatg ctctcctctc
     1681 atcaggtagc tggacagaaa tctagatgtt gttgctctct ctcaagatac cacatgggac
     1741 ggtcgttctc tgtgcatcgt cgcctttgac cggtgattct gagtggaaag gcccctgcct
     1801 caatcttctc aaggacggca tcacggtgcg agggtacgca cgacctgggt gctcgccagc
     1861 accttgcctc cgcgcaggac ccacctgggc ggctgttgca ggcgcagcac ttctcgcagg
     1921 gtgggagcgg ccagcacatt cagatctgct ttgcagcctg gttccagtcc gtagtccggc
     1981 aggcgcaggg cgcgagcggc gttgtaggtg acgaagtcga aggcggcctc gatctcgctg
     2041 ggcgcggcca gatgagcggt gtggcacagg taggctgcca cctcctgctg gtcgttgcgc
     2101 ccaaaggggt agaaggggtc agctacgtcg tcctgggagg agaagacgtt gacaccgtgc
     2161 tgccagagct gcttgacacg cgtgatgccc cggcggacgg gttcctgatc gtagcggcct
     2221 tgcacgacca ggctgatgtg cgggttgctg ctgatgctga cctctgcctg cttgagcagc
     2281 tccatgacct tgtgggcgtg cacctcgttg taggccgcca gcgctccaca gtggctggcc
     2341 gaaacccgcc cctggtagcc ctcgcgcagc gtcttgaccg ccaggtactc cagcgagcgg
     2401 ctgagtgggt tgtcggtatc gtcgatcagc atgtggatgt ccttattgta gcgcctggcc
     2461 agcgcgaagc agaagtcgat gtgcgtccgc gcgtcctcgt cgagcagttc gaaccagggt
     2521 agcccgccca ccacatcagc gcccagacgc atggcttctt ccagcagttc ggcggttcca
     2581 ggatcgcgca ggatgccctc ctgcgggaag gtcaccacct cgatatgcgc ctgatcccgc
     2641 ctttgctcgc gcaacgccag caggccttcc agcccgcgta gctggccgat actgtccacg
     2701 tcggcgaagg cgcggaagac ggtcgtccca ttgaggagac cctctcgaat ggcccggccc
     2761 gcgcgttcga ggatgtcctc ggtggtgtag cgcgccttgt gctcccaggt tagctccagg
     2821 cactcgccga aggtgtagtc tttgttgggg cgcatcgtct cttgcaggaa gcatttgtcg
     2881 aggtggatgt gcccgttgac ataggtcggc agcgccaggt tcccggcggc gtccacctcg
     2941 gttgtcgcct cgtcgggcag gtgttcggcg actgccgcga tgcgatcacc agtaatcccg
     3001 atgtcccagg tactctcgcg gcctcgcagg tgcgcctgcc gaatgattgt atccatcggt
     3061 cactccttcc ctaatatcgc ttccggtatt cccgccgtgt gaacgtcgca acgtagttga
     3121 tctcaatata gcattacaaa caacccttat tcaggccatg atacttcttc aaacagcccc
     3181 tttggcacag acgcaagcac ttcattgtct ttcgacgcat ctaccagaca gtagaagcgc
     3241 tgtctcgccc gtgtgacact tacataccaa cgatgtctcg ctttcaatgc gtccgcaccc
     3301 aatagaaata cagtatcgaa gtcctggctc ttcgcattat tgagagtggc aactaccagc
     3361 tttctgtgag cttgattttc ctcgatgaag gtgatgtagc tatcgatatg ctcaagcgct
     3421 tcctgaactg atttctcctc tagtttgctc aactcctcct cgatctcgtt cttttcctga
     3481 atatactcat cggtctgttc tcgtgcatcg gactggttgg ccaactcatc ccgcatagct
     3541 tgaatggact gccagacagc cttacatgtg ctgtctggag aatgtttccg aatgatggca
     3601 acatgctgct gtagcaccgc cagatgttct tctgtagctc ctatttgttc acatacaccc
     3661 ggctcaagtg cggcgtccag cagtttcatt ccaagccttc gggcgatttc gttcagcctg
     3721 gcaatctggc tggtactgaa atatggactg acgcaatgcc gtagcagtcg ctccatctca
     3781 ttggtttgat tgctgtcctg gatcaggcga aaataggtga gtacacgcct ggcgtgatag
     3841 ctctggtagc ggtgctggct ttctagcacc gcaaataggc atcctctcct cttcaaacac
     3901 tgttgtaccg gataggacag gtcactcctg gcaacgagga acgcgacact tccagcgacc
     3961 tctggcggca atgatcccat atgatacagt gcggcatcta ccatagccac tatgaggctg
     4021 cctgtacttc tttcatgctc tgaggcaaaa agagtgtttg gatctatacg gataactgtg
     4081 ggctttattc ccttcgagcc gagcgctgat tgttgatgtt cccactgaaa agatgatagt
     4141 acggcacacg caaaatccag aatatcttgt accgagcgga aattagttgt taagcgatgc
     4201 tcttgtacag gcatatcatt gcctaccagc tcgaacgtag cggaaagtgg gacctgttct
     4261 ttcgggttat cgagtattcg ttgtctcatg tccccgacag ccattacgtt tcgatgcgta
     4321 tgactcagga ttccgatcat ctcagcttcg gcaggggtga agtcctggaa ctcgtcgatg
     4381 atgatatgct cgtacttgcc ctgccaccgc tttgctatat ccgggttggc tctcaggata
     4441 acaagagcga aaagaacctg gtcatcgtag gtgtacactc cctgctcgcc ctggacgaac
     4501 tgataagcaa taaatgtagc atagtaaaac gccaacttac gcagcagcca ggcatttcta
     4561 ttcccaatgc ccttcagtgt ggtaagaagc tcctcattgt cagaaatact ggcaggataa
     4621 atacccagag tccggaacag cgatatggcc ttgaggcaat cacgctctgt agaagcgttt
     4681 ggcctgtaga tgccgagaag tcgaagaggc tccatatgtg caggaagata ctctagcatc
     4741 ccgtttgctc tataaagcct atctaacgcc tgcgaaacga gcgccagctc tctttcatcg
     4801 acttcttgcc aggtacccgt caataactga atggcttttc cctctggaaa gtaaggttgc
     4861 ccactcacct gcttgagtag ctgcacatgt tgtctgaaaa tgcctgtact tatcgattgt
     4921 agtgtttttg ttggtatcgg tctccctcca ctttgcaggt agtcgagctg atttcccaca
     4981 cgatcaacgg cgctatgaaa gtgagcagtg acaaggatac gctcggcggg aacaaggtga
     5041 tactcaacga ggtaacggaa atgctcctga agaagcgtcg tttttccgct cccggccggc
     5101 ccttgaatca ggtggtaccc ttcagagagc cttacgatcg cacgctgctc gtctgtcagg
     5161 tcctggcgtg ggacgaatgg ttcgatgagc ggctgttctc ccaaaaaata ggaccgtagc
     5221 agttgcccgc gtcgttctgc cggtgagagc gcgtattcct gcatgagcgc gagctttctc
     5281 tcttcgcagc gttcaacctg gcgcagcgag gtgaggtcga tcttgatctc atccgaagag
     5341 gcggcctctt gcaggaagtt cacgcgcatg acgacctctc cccgctcttc tgcagggaaa
     5401 gtgagtacgc ccagtacact gagcaatcgt agcgacctgt ttagc
//
LOCUS       DASZAK010000707         5306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_193899, whole genome shotgun sequence.
ACCESSION   DASZAK010000707 DASZAK010000000
VERSION     DASZAK010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5306
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193899"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..1129
                     /gene="rpsB"
                     /locus_tag="VGT44_12460"
     CDS             287..1129
                     /gene="rpsB"
                     /locus_tag="VGT44_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV2581657.1"
                     /translation="MANIISMKALLEAGVHFGHQTRRWNPKMRPFIFTDRNGIHIIDL
                     QQTVSRLNDAYKFIEQLAAGGGTVLFVGTKKQAQEAVAEEAKRCNMYYVNQRWLGGML
                     TNFQTMQSRIRYLRDLEARRDRGDFERLPKKEAQRLTDDITRLERTLGGIKDMRRLPN
                     ALFIVDTRKERTAVLEARRLEIPIVALADTNCDPDEMDYPIPANDDAIRAVRLLCARI
                     ADAVVEGRRSLEAQQKDSEPTESEEPVESAETSAPAEPAADESAQFDLATPNAGDLEL
                     VADK"
     gene            1416..2012
                     /locus_tag="VGT44_12465"
     CDS             1416..2012
                     /locus_tag="VGT44_12465"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581658.1"
                     /translation="MDYSAADVKKLRDETGATFADCKNALTEANSWGEAVKLLNARSD
                     HKAEKMMAAGRETKQGGVFSYVHHNHSVGVLLELDCSTDFVARSEAFRHLASELSLQI
                     AGVAPKYINFEDVPADVIAQARNAILEDESMARVPEARREDVVAGKLKKQLGEQVLMM
                     QPWVKDEAIIVGDLVKKVIAETGENIVLRRFSRFAVGE"
     gene            2083..2835
                     /gene="pyrH"
                     /locus_tag="VGT44_12470"
     CDS             2083..2835
                     /gene="pyrH"
                     /locus_tag="VGT44_12470"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV2581659.1"
                     /translation="MSQETSLAYSQVLLKLSGEALCPRKGFGIDAEMVRSVAGEIKQV
                     YELGVKIAIVIGGGNIWRGADAAATGIDRATADYIGMLATVMNALAMQDAMEKMGMVT
                     RVQTAIEMPPVAEPFIRRRAERHLEKGRVVILAAGSGNPFFTTDTAAALRAVELNAQV
                     LLKATKVDGVYDDDPMRNPNARKFDELSYMHALTMDLKVMDSTALSLCKDNHLPIVVF
                     DLNDKGALEAIIRGQKRGTLVHDIEEPEWSAR"
     gene            2900..3457
                     /gene="frr"
                     /locus_tag="VGT44_12475"
     CDS             2900..3457
                     /gene="frr"
                     /locus_tag="VGT44_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV2581660.1"
                     /translation="MADDLIGDAERRMQKAVEALKQDISAIRTGRASSALIERITVDY
                     YGAPTPINQVASISIPEARLLVIQPWDRKLLTDIEKAIQKSDLGINPNNDGQVIRLAI
                     PPLNEERRREMVKTLHKKLDEHKVAVRNVRRDVHDKFRDREKKKEITEDELKRSVDRL
                     QKLTDRYIDEMDKVGKTKEQEILEV"
     gene            3460..4245
                     /gene="uppS"
                     /locus_tag="VGT44_12480"
     CDS             3460..4245
                     /gene="uppS"
                     /locus_tag="VGT44_12480"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEV2581661.1"
                     /translation="MARLQNYFSSKGNGTATSRAATVEHSHAEQEPLVRHLAIIMDGN
                     GRWATRRHLPRLAGHKAGVNALRRVLELAVDEHIDMLSVYAFSTENWGRPRVEVEGLM
                     RLFWETIRSDIEKLHREGVRLRHLGRLQDLSPDIQRAVSDSVELTRNNTRIGLNVCFN
                     YGGRAELVDAVRQIIADGRDPDTITEELISSYLYTRDLPDPDMVIRTAGEMRVSNFLL
                     WQSAYSEYYATPTLWPDFGKDDLRAALESYRQRQRRFGRLASS"
     gene            4482..>5306
                     /locus_tag="VGT44_12485"
     CDS             4482..>5306
                     /locus_tag="VGT44_12485"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV2581662.1"
                     /translation="MQPASQTELQANVTEKAERQWYRSVGLRTITSFIGIPIVLACVW
                     FGGWVAFGAVTLIAVLAAYEVHAMMLHEGFHPLIGISLILSLLFLIAAMFPQQRGLLL
                     QIGLALALFVTFPLLFFRKKLEGAMVDWSLTLAIAVYLGWPLSLFPLIRGYQPGISNG
                     FWWLVTLLGGVWGFDTGAFFVGHFLGRHKLAPRISPAKTWEGAFGGLIFSIAAALLFA
                     MWPLGVPWYLALLLGLLIAVAATLGDLAESLIKRQTHVKDSGQFIPGHGGMLDRIDS"
BASE COUNT         1171 a         1524 c         1540 g         1071 t
ORIGIN      
        1 acttcgcagt catggtaagg gcgtaccctt gtggtcgccc ttcgtcccag tttccgcaaa
       61 ttttgcttga atcttcccct ctactatgtt atactgccca ttcgaacggg cgaccatgcc
      121 cgctcgcaga ggcagattaa ataaatacac acgctcttga atcggcaaaa accctggtac
      181 acctcatgaa gtaactgtgg taggggttga gtgaagttca cctcaatcaa ggggaacctg
      241 tttgccgggt gaaaagagcg gaggaccaaa agggaggttc cggtttttgg caaacattat
      301 ctcaatgaaa gcgcttctcg aagcgggggt tcacttcggg catcagaccc gtcgttggaa
      361 ccccaaaatg cggccattca ttttcacaga ccgcaatggc atccacatta tcgacctgca
      421 gcagaccgtc tcgcgcctga acgatgccta taagttcatt gaacagcttg cggcaggcgg
      481 cggcacggta ctcttcgtcg gcaccaagaa gcaggcgcag gaagcggtgg ctgaagaagc
      541 caaacgctgc aacatgtatt acgtcaacca gcgctggctg ggtggaatgc tcaccaactt
      601 ccagacgatg caatctcgca ttcgctacct gcgcgacctg gaagcccggc gcgatcgcgg
      661 cgactttgag cgcctgccca agaaagaagc ccagcgcctg accgacgaca tcacccgcct
      721 ggagcgtacg ctcggcggca tcaaggatat gcgccggttg cccaacgcgc tcttcatcgt
      781 cgatacacgc aaagagcgca ccgccgtgct ggaagcgcgc cgcctggaaa ttcccattgt
      841 cgccctggcc gacaccaatt gcgatcccga tgagatggat tatcctatcc cggccaacga
      901 cgatgccatt cgcgccgtgc gcctgctctg cgcccgcata gcagacgccg ttgtcgaggg
      961 acgccgctcg cttgaagcgc agcagaagga tagcgaaccc accgaaagcg aagagccggt
     1021 cgagtcagcc gagacgagcg ccccggcaga acccgctgcc gacgaatctg cccagtttga
     1081 tctggcaacc ccaaacgccg gtgatctgga acttgtcgct gataagtagg cggctagaac
     1141 gatggtagcc agggcgaccg ccagggtcgc ccctaccctg tgacgattac ccattttgcc
     1201 cgcatagggt aggggcgacc ctggcggtcg ccctggaaaa taaacatggc gggagaccgg
     1261 gaaagtgttc ccatctcccg cttcatcttg ttgagaagaa ctatagtagg cacatttgtc
     1321 aagcactcag gtgcaaggtt gcgtggtcag gagcgattgt gctttcccca cttgcctgag
     1381 tatcatcata agtgagattg aggagtatac caatcatgga ctattctgcc gcagatgtca
     1441 aaaagttacg tgacgagacc ggcgcgacct tcgccgactg taagaatgcc ttaaccgagg
     1501 ctaacagctg gggtgaagca gtcaaactgc tcaatgccag gagcgatcac aaggccgaga
     1561 agatgatggc tgccggtcgc gagaccaagc aggggggagt cttctcctac gtgcatcaca
     1621 accacagcgt cggtgtgctg ctggaactcg actgcagcac cgacttcgtg gcgcgcagcg
     1681 aggcgttccg ccaccttgcc agcgaactgt cgctgcaaat cgcgggcgtc gccccgaagt
     1741 acatcaactt tgaggatgta cccgccgatg tgattgcgca ggcgcgcaat gctatcctgg
     1801 aggacgagtc tatggcgcgt gttccagagg cacggcgcga ggatgtggtc gcgggcaagc
     1861 tcaagaagca gttgggcgag caggtcttga tgatgcagcc ctgggtgaag gacgaggcca
     1921 tcattgtcgg cgacctggtg aaaaaggtta tcgccgaaac aggtgagaac atcgtactgc
     1981 gtcgcttcag ccgattcgcc gttggcgaat aactgctgga aaggggagca ttcgttgcga
     2041 atgttcctcc cgtgatgagc acgtatggga gtatcacacg tcatgtcgca agagacatcc
     2101 ctggcctatt cgcaagtgct gctcaagctg agtggcgagg cgctttgccc gcgcaagggg
     2161 ttcggcatcg atgctgaaat ggtgcggagc gtcgccggcg agatcaaaca agtctatgaa
     2221 ttgggtgtga agattgctat cgtgatcggc ggaggtaata tctggcgcgg cgccgatgcc
     2281 gccgccaccg gcattgaccg ggccaccgcc gactacatcg gaatgctggc gaccgtcatg
     2341 aacgcgctcg ccatgcagga cgcgatggag aaaatgggca tggtgacacg ggtgcagacc
     2401 gctatcgaga tgccacccgt cgccgagccg ttcattcgcc gccgggctga gcgccacctc
     2461 gaaaaggggc gcgtggtcat tctcgcggcg ggcagcggca atcccttctt cacaaccgac
     2521 acagcggcag cgctgcgtgc ggtcgaactc aatgctcaag tgttgttgaa ggcaactaaa
     2581 gtggatggag tctatgacga tgatcccatg cgcaatccca acgcccgtaa gttcgatgag
     2641 ttgagctaca tgcacgcact cacgatggac ctgaaagtta tggatagcac ggcgctgtcg
     2701 ctctgcaaag ataatcacct tccaatcgtc gtctttgatc tcaacgacaa aggggcgctg
     2761 gaagcgatta ttcgtggcca gaaacgcggt acgctcgttc atgacatcga ggaaccggag
     2821 tggagtgcca ggtaacagaa gttacctcaa cactgataag cgcgcaggtc attgcgctgg
     2881 tacgaaaggg aacattgtta tggcagacga tctcatagga gatgccgaac gacgtatgca
     2941 gaaagcggtt gaggcgctca agcaggatat cagtgcgatt cgcaccggaa gggccagctc
     3001 ggccttgatc gagcggatta ccgtcgatta ttatggggcg ccgacgccaa tcaaccaggt
     3061 cgcctcgata tcgataccgg aggcgcgcct gctggtgatc cagccctggg accgcaaatt
     3121 gctgaccgac atcgaaaagg ctatccagaa atcggacctg ggcatcaacc ccaacaatga
     3181 tgggcaggtg attcgcctgg ctattccccc gctcaacgaa gagcgccgtc gtgaaatggt
     3241 caagaccctc cacaagaaac tggacgaaca caaggttgcc gttcgcaacg tgcgtcgtga
     3301 tgttcacgat aagtttcgcg atcgtgaaaa gaaaaaagaa attaccgaag atgagctgaa
     3361 acgcagtgtc gatcgcctgc agaagctcac tgaccgctac atcgatgaga tggacaaggt
     3421 cggtaaaacg aaggaacagg agatactgga ggtctaacct tggccaggct gcaaaactat
     3481 ttctcctcaa agggaaatgg gacggcgact tcgcgagccg cgacagtcga gcattcgcac
     3541 gctgagcaag aacccttagt gcgtcatctt gcaatcatta tggacggcaa tggccgctgg
     3601 gctacccgtc gccatctgcc gcgtctggca ggacacaagg cgggcgtaaa tgctctgcgg
     3661 cgcgttctcg aactcgcggt agatgaacat atcgacatgt tgagcgtcta cgctttttcc
     3721 accgagaatt gggggcggcc acgtgtggag gtcgaagggc tgatgcgcct cttctgggag
     3781 acgatccgca gcgatatcga gaagctgcat cgcgagggtg tgcgcctgcg ccaccttggc
     3841 cgcttgcagg acctctcgcc cgacatccag cgcgccgtca gcgactcggt tgaactgacg
     3901 cgcaacaata cccgcatcgg cctgaacgtc tgtttcaact atggcggacg cgccgagctg
     3961 gttgatgccg tgcgccagat catcgccgat ggccgcgacc ccgacacgat taccgaggaa
     4021 ctgatcagtt cctatctcta tacccgcgat ctacccgatc ccgatatggt tattcgcacg
     4081 gcgggcgaga tgcgcgtcag taatttcctg ctctggcaat cggcctacag cgaatattac
     4141 gcgaccccga ccctctggcc cgatttcggg aaggatgacc tgcgcgccgc gctcgaaagt
     4201 taccgccagc gtcagcgccg ttttggacga ctcgcctcat catgagctat aatagagcga
     4261 gaagtccaaa tacgaatgct gggttgttct atgtatcacg atgtcgtggg gacgcgataa
     4321 taaactttta caatcaaaac ctgtcatgtc attctgagcg cagcgaagaa tctcggtcct
     4381 gacgagtgag attcttcgct gggctcagaa tgacattgcc gactttggtt gtggaaattc
     4441 atcattgcgt accaacaaga taacccgcgc tgaggttctt cgtgcagcca gccagtcaga
     4501 cagagctaca agcgaatgtg accgaaaagg ccgaacggca gtggtatcgc tccgtcggcc
     4561 tgcgtaccat tacgtccttc attggcatcc cgatcgtgct ggcctgcgtc tggttcggcg
     4621 gttgggtggc atttggcgct gtgacgctga tcgctgtcct ggctgcctac gaggtccacg
     4681 ccatgatgct gcacgagggc ttccatcccc tgatcggcat cagcctgatt ttgagcctgc
     4741 tcttcctgat tgctgccatg ttcccgcagc agcgcgggct attactccaa attggcctgg
     4801 ccctcgctct gttcgtcacg ttcccgctgc tcttcttccg caaaaagctg gagggagcga
     4861 tggtggactg gtcgctgaca ctggctatcg ccgtctacct gggctggccg ctcagcctgt
     4921 ttccgctcat tcgcggctac cagcctggta tcagcaacgg cttctggtgg ctcgtgacgc
     4981 tgctgggagg cgtatggggc ttcgacacag gtgccttctt cgtcggccat ttcctgggcc
     5041 gccacaaact cgcgccgcgt atcagccccg ccaaaacctg ggagggtgcg ttcggcggcc
     5101 tgatcttctc tatcgcagcc gccctgctct tcgccatgtg gcccctgggc gttccctggt
     5161 acctcgccct gctgctgggc ctgctgatcg ccgtcgccgc cacgctcggc gacctggccg
     5221 agtcgctcat caaacgccag acccacgtca aagacagtgg ccagttcata cccggccacg
     5281 gcggtatgct cgaccgcatc gatagc
//
LOCUS       DASZAK010000708         3111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_658114, whole genome shotgun sequence.
ACCESSION   DASZAK010000708 DASZAK010000000
VERSION     DASZAK010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_658114"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..702
                     /locus_tag="VGT44_12490"
     CDS             <1..702
                     /locus_tag="VGT44_12490"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEV2581663.1"
                     /translation="RSLPADSSPPSDPSSPAPPRLEEPVWLEPFPDALLVDPQSDPED
                     QAVRQEQISLAFLVALQHLTPAQRAVLLLREVLEWKASEVAEWLDLSVPAVNSALQRA
                     RQTLQQRSKENGSETPVAQPRPQLQNLLDRYMTLWQQADVPGLVALLREDAWLTMPPL
                     PAWFRDERILLLCSGSGSSLPANNGGCCQRAPMAARLSGSIGGKVGKTADSRLPCGSW
                     GWKANRSAASLCSWM"
     gene            complement(789..2225)
                     /locus_tag="VGT44_12495"
     CDS             complement(789..2225)
                     /locus_tag="VGT44_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEV2581664.1"
                     /translation="MQNSHEKARQTWILVLASLASFMVSLDALVVVTALTTIHRDLGV
                     SIEALEWTLNAYTLSFAVLLIPAAALGDRFGRRRMFVAGLLLFVVASAACALAHNIGW
                     LIAARTIQGCGAALVLPLALTLLSAAFPPEQRARALGIFGSISGLALIAGPVVGGFIA
                     QGLAWQWIFWLNVPIGLLIVALVLSRVQESFGPRTSLDLGGLLSVSGASLGLVWGLVR
                     GNSAGWGSFEVKASLVAGGLLAIVFVAWELRARAPMVPMHFFRSRAFSAGNAAIFLQT
                     TSLVGVVFFLAQFFQVAQGNGPLGAGLRLLPLTATLFVVAPLAGVLVNRIGERVLVAG
                     GLFLQAIGIAWIGLVAAPGLPYAELIAPLIIAGCGVSLATPAMQNAVINAVRAGDIGK
                     ASGAFSMFRQLGGAFGVAILAAVFAATGSLGSAQAFSSGFASALSVAAALSLLGAIAG
                     LVLPGRQVKAIEPEESKTAALHEEALSP"
     gene            complement(2664..>3111)
                     /locus_tag="VGT44_12500"
     CDS             complement(2664..>3111)
                     /locus_tag="VGT44_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581665.1"
                     /translation="FMATIQTILQPGVGEEIILNQNAFIEQVLPAQITRSLTEAEMNA
                     YRVPFPTPESRRPILDNGPRQFPAAFELVGSYSEWLTTTDVPMLHLYVTPGLINPEAS
                     VQWSRTHIHTIEQQYMGVGGHFFQEDHAVEIGAAIRDWLGRVVSAH"
BASE COUNT          644 a          955 c          960 g          552 t
ORIGIN      
        1 cgctccctgc cagcagacag ctctcccccg agcgatccca gcagccctgc gccgccacgg
       61 ctcgaggagc cggtctggct ggagccgttc ccggatgcac tgctggttga tccgcagagt
      121 gatccggaag atcaggcggt gcggcaggag cagatcagcc tggcgtttct ggtcgcgtta
      181 cagcacttaa ctccagccca gcgggccgtt ctgctcttgc gcgaggtgct ggagtggaag
      241 gcctctgaag tggccgagtg gctagacctc tccgttcccg ccgtgaactc cgctttgcag
      301 cgcgcccgac agacgttgca gcagcgctct aaagaaaacg gaagcgagac tccggttgcg
      361 cagcctcgcc cccaattaca aaacctgctt gatcgctaca tgaccctgtg gcagcaggcg
      421 gatgttcctg gcctggtggc gctgctgcga gaggatgcat ggttgaccat gccgccgctt
      481 cccgcctggt tcagggacga acggatattg ctactctgct ccggctccgg gtcttcactc
      541 ccagcaaaca atggcggctg ctgccaacgt gcgccaatgg ctgcccggct ttcgggctct
      601 atcggaggga aggtggggaa gacggctgat agccgtcttc cctgtgggtc ctgggggtgg
      661 aaggcgaaca gatcggcagc atcgttgtgt tcctggatgt gaccagtttc acagcttttg
      721 gcctcccgtc caccattgaa ggggaagcgg gaggccaagc agcatgaaga tcggttgtat
      781 ggaggatttt acgggctcag agcttcttcg tgcagagcgg ctgttttact ctcctctggc
      841 tcaattgctt tgacctgcct gccgggcagc accaggccgg cgatcgctcc caggagcgag
      901 agggcagcgg cgacgctcag cgcgctcgcg aagccgctgc tgaaagcctg ggcggagccg
      961 aggctgccgg tcgcggcaaa caccgccgcc aggatcgcca cgccgaacgc accgccaagc
     1021 tgacggaaca tgctgaaagc gccggaagcc ttgccgatgt cgcctgctct caccgcgttg
     1081 attacggcat tctgcatggc gggtgtggcc agcgagacgc cacagccggc gatgatcagc
     1141 ggtgcaatca gctccgcgta gggcaggccg ggcgcggcga ccagcccgat ccaggcgatc
     1201 ccgatcgctt gcaggaacag cccgccggcc accaggaccc gttcaccgat ccggttgacg
     1261 agaacaccgg ccagcggggc aaccacgaac agggtcgcgg tcaagggcag caggcgtagc
     1321 ccggcgccca gcggtccatt gccctgcgcg acttgaaaaa actgggccag aaagaagacg
     1381 acaccgacca acgacgtggt ctgcaagaag atcgccgcgt tgcctgcaga gaaggcgcga
     1441 gagcggaaga agtgcattgg caccattggc gcgcgagcac gcagttccca ggcaacgaag
     1501 acgatggcga gcagcccacc ggccacaagc gaggctttga cctcaaagct gccccagccg
     1561 gcgctgttcc cgcgcaccag cccccacacc agcccgaggg aggcgcccga aacgctcagc
     1621 aggccgccga gatcgagcga agtgcgtggc ccgaagcttt cctgtacgcg actgagcacg
     1681 agcgcgacaa tcagcaggcc gataggcacg ttgagccaga agatccactg ccaggccagg
     1741 ccctgagcga taaaccctcc cacgaccgga ccggcgatca gggccagccc gctgatgcta
     1801 ccaaagattc ccagcgcccg cgcccgctgc tccggtggga aggccgcgct cagcagcgtc
     1861 aacgccaacg ggagcaccag cgctgcgccg catccctgga tggtgcgcgc ggcgatcagc
     1921 caaccaatgt tgtgagcgag cgcgcaggca gccgatgcta cgacaaacag caggaggcca
     1981 gcgacgaaca tgcgccgccg cccgaagcgg tcacccaggg cagcagccgg aatgagcagc
     2041 accgcgaagc ttagggtata ggcattcagg gtccattcta acgcctcgat agaaacaccc
     2101 aggtcccgat gaatcgtggt aagcgcggtc acgacgacca gggcatcgag cgagaccata
     2161 aatgatgcca gcgaggccag caccagaatc caggtttgtc tggctttttc gtgagagttt
     2221 tgcatgataa aatggtgtcc tttcattggt ttgcgagagg tgtcaatcac ctttaagaga
     2281 aagagtctgc gatggccttc agcattccct catgagagag gacctgcgca aaggcgacca
     2341 cttctcccat ccaggatggg gttgcaggct tcgattccgg cgtaatctgg attcttcccg
     2401 aagaacgcgt catactgcga gggtgtcctc cttgtttcag aagcgattgt ccggtctgtg
     2461 gtatcctaga cagaggaaag tgagacatcg aacattcagc tttccagggg ggaatgctca
     2521 tgcttcctgc gcagacgcaa aagctggctc aactccctgt ctctcctttt ggattgtcag
     2581 gacgaggcgc agcttcagtc gctctctgaa acgagccggg agagcagttc atcccggctc
     2641 gacatctccc ggtatcgcct gatttagtgt gcgctgacga ctcgccctaa ccaatctcga
     2701 attgcagcac caatttctac agcatggtct tcctggaaga agtgcccgcc tacgcccata
     2761 tactgctgct caatggtgtg aatgtgcgtt cggctccatt gcacactggc ttcagggtta
     2821 atcagacccg gcgtgacgta caggtgaagc atcgggacat cggtcgttgt cagccactca
     2881 gaatagctgc cgaccagctc aaacgcggcg gggaactgac gtggaccatt gtccagaatc
     2941 gggcggcggc tctccggcgt cggaaacggc acgcggtagg cattcatctc tgcctcggtc
     3001 agcgaacggg tgatctgcgc tggcagaacc tgctcaatga aggcattctg attgaggatg
     3061 atctcttcac cgacgccggg ctgaaggatc gtttggatag tcgccatgaa c
//
LOCUS       DASZAK010000709         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_374177, whole genome shotgun sequence.
ACCESSION   DASZAK010000709 DASZAK010000000
VERSION     DASZAK010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374177"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VGT44_12505"
     CDS             complement(<1..989)
                     /locus_tag="VGT44_12505"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2581666.1"
                     /translation="MPGNQGAKGTYGGAQRGTHGAQGGQKKQVAVVQESFIGKSLHQG
                     EYVIEASIGRGGMGQVFLASHRTLDVPVAIKQARADQPLPESVQKELDTLLAQEPENA
                     PGRSGHTAALDFPLSGGAHTDRFLREALLLARLRHPAIPTLYDYFLEDGYWYLVMEYV
                     PGPTLASYIRQNAPLPPLEALNYTMQLCDVLDYLQQQTPPVVFRDLKPSNVILSPEGR
                     LALVDFGIARYFKEGQINDTTDFGSPGYASPEQYEGSGQTDGRSDVYSLGVILHEMLS
                     GKRPARGGSRGSQPEALRRINPALSPALSGLVAVATRPEPMYRFQTARAFYLAL"
     gene            complement(1116..>1538)
                     /locus_tag="VGT44_12510"
     CDS             complement(1116..>1538)
                     /locus_tag="VGT44_12510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581667.1"
                     /translation="TVVSTFPSEYIYDPDVIDEVARVLRPGGRLVVVEGANLLPIGLL
                     QPFLMLIQFLVYGPSAVYGPRPRHNLDEEVERSRRSDSSDPTIMHVNRDTGSDTTTEV
                     VQDAWFGQQIPLEQHGLRRRSERVRSRRWEVYVTIGEK"
BASE COUNT          319 a          462 c          446 g          311 t
ORIGIN      
        1 agcgcgagat aaaaggcacg agcggtctga aagcggtaca tgggttccgg gcgcgtagca
       61 actgccacca gcccgctcaa ggccggcgaa agtgccgggt tgatccggcg cagcgcctcg
      121 ggctggctgc cccgcgaacc gcccctggcc gggcgtttgc cgctgagcat ttcgtgcaag
      181 atcacgccca ggctatagac atcagagcgg ccatccgtct gcccgctccc ttcatactgc
      241 tctggcgagg cataaccggg cgagccgaaa tcggtggtat cgttaatctg cccctctttg
      301 aaatagcgcg cgatgccgaa atcgaccagc gccaggcggc cttcaggcga aagaatcacg
      361 ttggatggct tgaggtcacg aaagacgacc ggcggcgtct gttgttgcag ataatccagc
      421 acatcgcaca actgcatggt gtaattgagc gcttcgagcg gcggcagtgg cgcattttgg
      481 cgaatatagc tggcaagcgt tggccctggc acatattcca tcaccaggta ccagtagcca
      541 tcttcgagga aatagtcgta gagcgtcggg attgccgggt ggcgcaggcg cgccagcagc
      601 agggcttcgc gcaggaaacg gtcggtgtgc gccccgccac ttaaaggaaa gtcgagcgct
      661 gccgtatggc cggaacgccc aggcgcattc tcaggctcct gtgcaagcag tgtatcgagt
      721 tccttttgca cactttcggg cagcggctga tccgcgcgcg cctgtttaat ggcgaccggg
      781 acatccagtg tgcggtgcga ggcaagaaag acctgcccca tacctccgcg acctatcgag
      841 gcttcaatca catactcgcc ctggtgcagt gacttgccga tgaagctctc ctgaacgacg
      901 gcaacctgtt tcttctgccc cccttgcgct ccatgagtcc cgcgttgcgc cccaccatat
      961 gttccctttg caccttgatt ccctggcatc acgcaccctc cctttcaaca ataccccttt
     1021 atcattcatg gtaatcaaat caaggcattc cgtcaacaag ggagcagtgt tatttcttcc
     1081 ccaaatagcc agggcgacca cgagggtcgc cctggctatt tctccccaat cgtgacatac
     1141 acctcccaac ggcggcttcg cacacgctcg gagcggcggc gcaggccgtg ttgttcgagg
     1201 ggaatctgct ggccaaacca cgcatcttgc acaacttcgg ttgtcgtatc cgagccggta
     1261 tcgcgattta cgtgcatgat cgtaggatcg gacgaatcgc ttctccggct gcgctcgacc
     1321 tcttcatcca ggttatggcg agggcgaggg ccgtaaacgg cggaggggcc atagaccagg
     1381 aattggatta gcataagaaa tggttgtaag aggccgatgg gaagcaggtt tgctccttcc
     1441 acaacaacca ggcgtccacc gggacgtagg acgcgcgcga cctcgtcgat cacatcgggg
     1501 tcatagatat attctgaggg gaaggtactg accaccgt
//
LOCUS       DASZAK010000710         2452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_445332, whole genome shotgun sequence.
ACCESSION   DASZAK010000710 DASZAK010000000
VERSION     DASZAK010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445332"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="dnaA"
                     /locus_tag="VGT44_12515"
     CDS             <1..957
                     /gene="dnaA"
                     /locus_tag="VGT44_12515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV2581668.1"
                     /translation="VASAPGDTYNPLFLYGGVGLGKTHLLHAIGHYARAAGRTVAYVT
                     AERFTNEIIEAIRLRTTDAFRLRFRAVDVLLVDDVQFIAGKESTEEEFFHTFNALHSA
                     NKQIVVTSDRPPKAIHSLQDRLRSRFEWGLLADIQAPEYEHRLAILRSKAESLRFAIP
                     SAVIEYIARPECSSVRELEGALNRVIAYATLHDAALSVSLAAQALGDIYDGGNKHTTN
                     LTLLDVLEGVCAYYNLSKEQMCGKQRDREIAWPRQVSMYLMRQETSASLLQIGGELGG
                     RDHTTIMHGWEKVQSEIAANDRVRREVAAVLESVQRGKQGKS"
     gene            935..2371
                     /locus_tag="VGT44_12520"
     CDS             935..2371
                     /locus_tag="VGT44_12520"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2581669.1"
                     /translation="MANKASRRGVAHSVITIIHWKRGPMPDALTSYLDSHIHDAQTLL
                     ERMARQPSIAAQNVGMAEMAEMVEGLLAETGFTTQRLYAEGAPVAIYGELKGSSDATV
                     LLYNHYDVQPAEPYELWQTPPFEPTMRDGKLYARGVSDNKGEIAARLTAIRSLHTVYG
                     TLPVTLHWIIEGEEEVGSPHFGAIAEQYSSLLQADGCLWEGDSFDASGRPEATLGAKG
                     LLYVQLDVEGTKTDAHSGNAPILPSAAWRLVQALATLRGPDGRVLIPGFYDSVRPPSP
                     EQLAAMADQTDSEAELREAYGISAFVDNLTGIQLRERYSFSPTCNIAGLLSGYTGEGS
                     KTVLPSKAMAKIDFRLVPDQTPEEILTKLRSHLDAQGYNDIHVTVFGRATPTVSSMSD
                     PFVRRILAIAESFNWKKPSITPLSGGSLPLLEPLSRFVGLPGLSAPGNATYWANGAHA
                     PNEHIRLEDLERAVRFNCHLFQSLGSQG"
BASE COUNT          463 a          758 c          758 g          473 t
ORIGIN      
        1 gtcgcctccg ccccgggcga tacttacaat ccgctctttc tctatggcgg cgtcggcctg
       61 ggcaagacgc atctgctcca cgccatcggg cactatgcgc gcgctgctgg gcggacggtg
      121 gcctatgtca ccgcggagcg cttcaccaac gagatcatcg aggccattcg cctgcgcacc
      181 accgatgcct tccgcctgcg ctttcgcgct gtggatgtgc tgctggtgga cgatgtgcag
      241 ttcatcgcgg gcaaagagtc taccgaggaa gagtttttcc ataccttcaa tgccctgcac
      301 agcgccaata agcagattgt tgtcaccagc gatcgcccgc ccaaggctat tcacagcctg
      361 caagaccgcc tgcgctcgcg tttcgagtgg gggctgctag cggatattca ggcgccggaa
      421 tacgaacatc gcctggcgat cctgcgctcg aaggcggagt cgctgcgctt cgccatcccg
      481 tctgcggtca tcgaatatat cgcgcgcccg gaatgcagca gcgtgcgcga actcgaaggc
      541 gcgttgaacc gcgtgatcgc ctacgccacg ctgcacgacg ccgccctctc cgtcagcctg
      601 gcggcgcagg cgcttggaga tatctacgat ggtggcaaca agcacacgac taatctgacg
      661 ctgctcgatg tgctggaagg cgtctgcgcc tactataatc tcagtaaaga gcagatgtgt
      721 ggtaagcagc gcgaccgtga gatcgcctgg ccgcgccagg tttctatgta tttgatgcgc
      781 caggagacga gcgcttcttt gctgcaaatc ggcggcgagc tgggcgggcg cgaccatacc
      841 accattatgc atggctggga aaaggtgcaa tccgaaattg ccgcgaacga ccgcgtgcgc
      901 cgcgaggtgg ctgccgtgct ggaatcggtg cagcgtggca aacaaggcaa gtcgtagggg
      961 cgttgcccat agcgtaatca ccatcatcca ctggaagcga ggtcctatgc ccgatgcgtt
     1021 gacatcctac cttgatagcc atatccacga cgctcagacc ctgctcgagc ggatggcgcg
     1081 ccagccgagt atcgccgctc agaacgtcgg catggcggag atggccgaga tggtcgaggg
     1141 gctgctggct gagacgggct ttacaacgca gcgcctgtac gcggaaggcg cgccggtcgc
     1201 gatctatggc gagctgaagg gaagtagcga cgcgacggtg ctgctctata accactacga
     1261 cgtgcagccc gccgagccgt atgaactgtg gcagactccg ccttttgagc cgaccatgcg
     1321 cgacggcaaa ctctacgcgc gcggcgtctc cgacaataag ggcgagattg ccgcgcgctt
     1381 gaccgccatt cgctccttgc ataccgtgta cggcacactg cccgtgacgc tgcactggat
     1441 catcgagggt gaagaagagg tcggcagccc gcatttcggc gcgattgccg aacagtacag
     1501 ctcgttgttg caagcggacg gctgtttgtg ggaaggcgat agtttcgatg cgagtggtcg
     1561 gccagaggct acgcttggcg cgaagggtct gctctatgtg cagcttgatg tcgaaggcac
     1621 aaagaccgac gcgcactcag gcaacgctcc tattctcccc agcgcggcct ggcggctggt
     1681 gcaggcgctt gcgacgctgc gcgggccgga tgggcgcgtc ctgatccccg gcttctacga
     1741 ctcggtgcgg ccaccttcgc cggagcagct ggcggcgatg gcggatcaga ccgatagcga
     1801 ggcggaactg cgcgaggctt atggcatctc ggcctttgtc gataacctga cgggcataca
     1861 actgcgcgag cgctactcct tctcgcccac ttgcaatatc gcgggcctgc tcagcggcta
     1921 taccggcgaa gggtcgaaga ccgtcctgcc gtcaaaggca atggcgaaaa tcgatttccg
     1981 gctcgtcccc gaccagactc ccgaagagat tctcaccaaa ctgcgctcgc acctggacgc
     2041 gcagggctac aacgatattc acgtcacggt ctttggccgg gccacgccga cggtgtcttc
     2101 tatgagcgac ccctttgtgc ggcgaatcct ggctatcgca gagtcattta attggaagaa
     2161 gccgtccatt acgccgctga gcggcgggtc gctgcccctg cttgagcctt tgagccgctt
     2221 tgtcggcctg cccggcctca gcgcgcccgg caatgccacc tactgggcca acggcgcgca
     2281 tgcccctaac gagcatattc gacttgagga cctggagcga gcggtgcgtt tcaattgcca
     2341 tctttttcaa agcttggggt ctcagggttg agatatggaa ctttcaaccc acgcccaacc
     2401 tcacggctgg gtgcaacccg gaggtgcctt gcacctagac gcctgatacc gg
//
LOCUS       DASZAK010000711         5807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_586838, whole genome shotgun sequence.
ACCESSION   DASZAK010000711 DASZAK010000000
VERSION     DASZAK010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586838"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VGT44_12525"
     CDS             <1..236
                     /locus_tag="VGT44_12525"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEV2581670.1"
                     /translation="PLYGMPILNADQAHTVLILKRSLSPGFAGEDNELFYDKKTMMVF
                     GDAKVTLTSLIQLLKKTAAAPSGSTKASAGIHS"
     gene            complement(227..1042)
                     /locus_tag="VGT44_12530"
     CDS             complement(227..1042)
                     /locus_tag="VGT44_12530"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEV2581671.1"
                     /translation="MNERQRAYQMITYPKLRRALALTLRSARRKPMTHGLIEVDVTKA
                     HEFLRNHKATTGESLSFTAFIITCLAHAVDEHKSVQTYRKGGKRLVLFDDVDVATLIE
                     REMAGQKQPIVYIIRAANTKTFREIHQEIRAAQMKAVEQAWEGLKDFGWLPLAVFKVF
                     WPVFWWMKGRSPSVQKKYGGTVGVSAVGMFGNGGGWGIPINDHTLDLTLGGIVEKPGV
                     IDGHIAIRDYLCITMSFDHSIIDGAPAARFSTRLRELIENGYGLINEPAMSYE"
     gene            complement(1102..1668)
                     /locus_tag="VGT44_12535"
     CDS             complement(1102..1668)
                     /locus_tag="VGT44_12535"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HEV2581672.1"
                     /translation="MQNISNQPQAPIEYEQGYAPEQQPEDNVGESPQSGYQPQNYPPG
                     QPYPPYYQGQQQQYWNQQPYIMAAPGDVSPFERTSLGMKARTAGLLCYLFGWVGGLVF
                     LLLERDNRFVRFHAIQSILFFGAVSILGWVFSYFPFALFGLGGIVGLIGFIGWIVLMV
                     AAHRGRYYKLPLFGDYAEQLANRVRINL"
     gene            2001..2282
                     /locus_tag="VGT44_12540"
     CDS             2001..2282
                     /locus_tag="VGT44_12540"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEV2581673.1"
                     /translation="MITQVEPKTFREELRVTGETLLTAVKKLAHEGNVRRIIIKQGEH
                     VVVELPLTVGVISVVLTPMLAAIGAISALLADCTIEVERIEPPVEVKVP"
     gene            complement(2401..2760)
                     /locus_tag="VGT44_12545"
     CDS             complement(2401..2760)
                     /locus_tag="VGT44_12545"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581674.1"
                     /translation="MDNPFAQEPKATTGWLWQVITGVALVFLLGLHIIANHFIAKGGL
                     RNFADVLAYLRNPVFLVLETLFLVVVASHAMLGIRAILMDFGLSDRVEKLLSRALTVV
                     GALTVGYGLWLTWAIAH"
     gene            complement(2765..3154)
                     /gene="sdhC"
                     /locus_tag="VGT44_12550"
     CDS             complement(2765..3154)
                     /gene="sdhC"
                     /locus_tag="VGT44_12550"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEV2581675.1"
                     /translation="MASLESPKVRRAGRWFDVRRRRLGMWAYALNRVTGIGLVVYLYL
                     HLVILSQLLGGAKSWDAFVSLARSPFFLTLDVVLIAGALIHGLNGLRVGLTGFGIGLK
                     AHKALFGALMVVAAIALFVAALLIYGG"
     gene            complement(3157..3927)
                     /locus_tag="VGT44_12555"
     CDS             complement(3157..3927)
                     /locus_tag="VGT44_12555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV2581676.1"
                     /translation="MIDLSLQIFRYKADQPPHYDTFTVQVAETAYVLDALDAVWATHD
                     HSLTYRRACHHSACGVCAMRINGVEKLPCVTRISEVWDGHSPLRLDPLRNFPVVSDLV
                     VDVKGLFQRMSASDLEITRDSEPTLPLSVDNLDELDVHVEPEPVEPPENLARFNRFES
                     CIECGICMSACPTVAADDKFLGPAGLAAIYYERQKTDDPREVAHLLALADGEHGVWRC
                     HSAYECTEACPQAVDPAGKIMALRRELVANRLKKGWRR"
     gene            complement(3924..5093)
                     /locus_tag="VGT44_12560"
     CDS             complement(3924..5093)
                     /locus_tag="VGT44_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2581677.1"
                     /translation="MSAPHIIIIGAGSTGSATAHDLALRGMRVTVIERGDIASGTTGR
                     THCLLHSGGRYCVNDPEAAAECIVENKAMRKIIPDLLELNDGLFVALTEADLAFKARF
                     LEGCAVSGIPARELPIKHALEIEPFLNPGALAAVQVPDAVFEPVRFCLAFLATAQQND
                     AVVRRFTEVTDFIQSGGCVTGVKVRDYRTGRTEMVGADLVVNAAGPWVGDIAAMAGVS
                     VPVTPTAGVMVTLDGRLNNMVLNRLNKPSDGDIIVPQRATSIIGTTSWVVADPDLIPV
                     PPDHVAKMIAQGEQLMPAVRKIPIRARVAAARPLIVKGGKDAREVSRTFECFDHKEDG
                     VDGFVTIAGGKMTTARGMAERVSDLVCHKLGIAADCRTRDVPLVSYRLFYQGGAQ"
     gene            5402..5590
                     /locus_tag="VGT44_12565"
     CDS             5402..5590
                     /locus_tag="VGT44_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581678.1"
                     /translation="MDYKTELDRLHAAGFTTREIARLYQFRRMHVANELDRAPVDLAR
                     LRFVRWLVENGRLTDQLV"
BASE COUNT         1223 a         1735 c         1698 g         1151 t
ORIGIN      
        1 gcccactcta cgggatgccc attctcaacg ccgaccaggc tcacactgtc ttgatcctga
       61 agcgcagttt atcgccaggc tttgctggcg aggacaacga actcttctac gataagaaaa
      121 ctatgatggt cttcggcgac gcgaaagtga ccctgacgag cctgatccag cttttaaaga
      181 aaactgcggc agcaccgtct ggctcaacaa aggcaagtgc gggaattcat tcataagaca
      241 tggccggttc attgatgagt ccatagccgt tttcgatcag ctctctcaag cgcgtactga
      301 agcgggctgc tggcgcaccg tcgattatgc tatgatcgaa gctcatcgtt atgcaaaggt
      361 agtcgcggat ggcgatgtgt ccatcgatga cgcctggctt ctcgacgatg ccgcccagcg
      421 taagatcaag ggtatggtcg ttgatcggga ttccccatcc gccgccattg ccaaacatgc
      481 cgactgccga gacaccgaca gttccaccgt acttcttctg cacagaaggg gacctgccct
      541 tcatccacca gaacaccggc cagaagactt tgaagaccgc cagcggcagc cagccaaaat
      601 ccttgaggcc ctcccaggcc tgctccactg ccttcatctg tgccgccctg atctcctgat
      661 gaatctcgcg aaaggtcttt gtattcgcgg ctcggatgat gtagacgatg ggttgttttt
      721 gccctgccat ctcacgctcg atcagagtcg ccacgtccac gtcatcaaac aggacgaggc
      781 gcttgccgcc cttgcgatac gtctgcacgg acttatgctc atcgactgca tgcgccaggc
      841 aggtgataat gaaggcggtg aacgacaggg actcccccgt ggtcgctttg tgattccgca
      901 agaactcgtg ggctttggtc acatcgactt cgatcaagcc atgcgtcatg ggcttgcgcc
      961 gggctgaacg aagcgtgagc gccaacgcac gccgcagctt tggatacgtg atcatctggt
     1021 atgcgcgctg gcgctcgttc atttccagct tctcctctct cagcctcaca aagctcctct
     1081 taggctagag gctcggtcgc tttagagatt gattctgacc cgattcgcca attgctcggc
     1141 atagtcaccg aacaggggga gcttatagta ccggccccgg tgcgccgcta ccatcagcac
     1201 aatccagccg atgaagccga tcagccccac gatcccgccc agcccaaaga gcgcaaaggg
     1261 gaagtagctg aaaacccacc ccaggatgct gacggcgcca aagaacagga tggattggat
     1321 cgcgtggaag cgcacgaagc gattgtctcg ctccagcagc aaaaagacca ggcctccaac
     1381 ccagccaaag aggtagcaga gcaatcctgc ggttcgagcc ttcatgccca acgatgttcg
     1441 ctcgaacgga ctcacatccc ctggtgccgc catgatatac ggctgctggt tccagtactg
     1501 ttgctgctga ccctgatagt acggtggata gggttgacct ggaggatagt tctgcggttg
     1561 atagccgctt tgcggacttt cgcctacgtt atcctccggt tgctgctccg gggcataccc
     1621 ttgttcgtac tcaatcggag cttgcggttg gttagaaatg ttttgcatgg ttcaccaaat
     1681 cttcccccgg tgaggtgtgt gcgagcaatc ccctcaccgg ctgattacat acctttcctc
     1741 aataagcata tgcgctcaac ctcacagaag cgctacaaat gctcatgtgt gcatcacgaa
     1801 cggctaacaa tatcccgcag acgccgaccg cgcatcgaga agagaggtag tgatgggccg
     1861 caaatggtga catacctgtg tcttttcagc gccaatttga gagcgatcag tgacggcaag
     1921 ggagtacgat agaaggtgga caccgtgggt gttcttgaaa ctgagtgtga aagactggtt
     1981 gatttcgaag gagaacgata atgataaccc aagtagaacc aaagacgttc cgcgaggaac
     2041 tgcgcgtgac gggtgagacg ctgctcaccg cagtaaagaa actcgcccac gagggcaatg
     2101 tccgccgtat tatcatcaaa caaggcgaac atgtcgttgt ggagctaccg ctgacggtcg
     2161 gagtgatcag cgttgtgctg acccccatgc tggccgcgat cggcgcgata agcgcattgc
     2221 tggcggattg caccatcgag gttgagcgga tcgagccgcc ggtagaggta aaagtaccat
     2281 agtgcctggg atagagagtt gagggtgcgc aaccggggat gtttcaatgt ctacgcgccg
     2341 ggaggcgtga gcattccttc ccggttgccg ccacccccaa tctgaaattg tccggatgct
     2401 tcaatgcgcg atggcccagg tcagccacag gccgtagcca accgtcagcg ctcccacgac
     2461 ggtgagcgcc cggctcaaca gcttctcgac gcggtcggac agcccaaaat ccatcagaat
     2521 ggcgcgaatg ccgagcatgg cgtggctggc gacgacgacc aggaacagcg tttcgaggac
     2581 gagaaacacc ggatttcgca agtacgcaag cacgtccgcg aaattgcgca gccctccttt
     2641 ggcgataaag tggttcgcga taatgtggag tccgagcagg aacaccaggg ccacgccggt
     2701 gatgacctgc cagagccagc cagtcgtggc ttttggctcc tgtgcgaatg gattgtccat
     2761 cgttctaccc tccgtagatc agcagcgccg cgacgaacag cgcaatagca gccacgacca
     2821 tcagagcgcc gaagagggct ttgtgcgcct tcaggccgat gccaaagccc gtcagcccca
     2881 cgcgcaggcc gttcagccca tgaatgagtg cgccggcgat caacacaaca tcgagcgtca
     2941 ggaaaaaggg cgagcgcgcc agcgagacga aggcgtccca cgatttagcc ccgcccagca
     3001 actggctcaa gatgacgagg tggagataca ggtagaccac caacccgatg ccggtgacgc
     3061 ggttgagggc atacgcccac atgcccagcc tgcgccgccg cacatcgaac cagcggcccg
     3121 cgcggcgcac tttgggagac tcaagacttg ccatcgttat cgcctccatc ctttcttgag
     3181 ccggttggcg accagctcgc ggcgcagcgc catgatcttg cctgcggggt cgacggcttg
     3241 cgggcaggcc tctgtgcatt cgtaggcgct gtgacagcgc cagacgccgt gttcgccgtc
     3301 cgccagagcc agcaggtgag ctacttcacg cgggtcatcg gttttctggc gctcgtagta
     3361 aatggccgcc agccctgctg gtcccaaaaa cttgtcgtcg gctgctacag tcgggcatgc
     3421 gctcatgcag atgccgcact cgatgcagct ctcaaaacga ttgaaacgcg ccaggttctc
     3481 cggcggctcg accggttcgg gttccacatg gacatccagc tcgtcgagat tgtcaacgga
     3541 gagtggcagc gtcggctccg agtcgcgcgt gatctccagg tcggaggccg acatgcgctg
     3601 gaacagaccc ttcacatcca cgaccaggtc gctgaccacc gggaagttgc gcagcgggtc
     3661 gaggcgcagc ggactatgcc cgtcccacac ctcgctgatg cgcgtgacgc agggcagctt
     3721 ctcaacgccg ttaatgcgca tcgcgcacac gccgcaggcg gaatgatggc aggcgcggcg
     3781 gtaggtcagg ctgtggtcgt gcgtcgccca caccgcgtcg agcgcatcga gaacgtaggc
     3841 tgtctccgcc acctgcaccg tgaatgtatc gtagtgaggc ggctggtccg ccttgtagcg
     3901 aaaaatttgc agcgataaat cgatcattgc gcacctccct ggtagaacaa gcgatacgag
     3961 acgagcggca cgtcgcgcgt gcggcaatcc gccgcaatac cgagcttgtg gcagactaaa
     4021 tcggagaccc gctcagccat gccgcgcgcg gtcgtcatct tccccccggc gatggtcacg
     4081 aagccatcca cgccgtcctc cttatgatcg aagcattcga aggtgcggct cacctcgcgc
     4141 gcatccttcc cgcctttgac gatcagcggg cgcgcggcag ccactctcgc gcggatcggt
     4201 atcttccgca cggctggcat caactgctcg ccctgggcga tcatcttcgc gacgtggtcg
     4261 ggcggaacgg ggatcaggtc cgggtctgcc acgacccacg aggttgtgcc gatgatcgag
     4321 gtggcgcgct gcggcacgat gatatcgcca tcggatggct tgttgaggcg gttcaagacc
     4381 atattgttca gccgcccgtc gagcgtcacc atgacacccg cagtcggcgt caccggcact
     4441 gaaacgccgg ccatcgcagc aatgtcgcca acccaggggc ctgcggcgtt gacgaccagg
     4501 tcggctccta ccatctcggt cctgcctgtc cggtaatccc tgaccttcac acccgtcacg
     4561 cagccgcccg attggatgaa gtcggtcact tcggtgaaac ggcgcacaac cgcgtcgttc
     4621 tgttgcgccg tcgcgaggaa ggccaggcag aagcgcaccg gctcaaacac ggcatcaggc
     4681 acctgcacgg cggcgagcgc gccagggttg aggaacggct ctatttccag ggcatgcttg
     4741 atgggtagtt cgcgcgccgg gatgccgctg actgcgcagc cctccagaaa acgcgctttg
     4801 aaggccagat cggcctccgt cagagcaacg aacagaccat cgttcaactc cagcagatca
     4861 gggatgatct tgcgcatggc cttgttctcg acgatacatt cagccgccgc ctccgggtcg
     4921 ttgacgcagt agcgcccgcc ggagtggagc aggcagtggg tgcgcccggt cgtcccggag
     4981 gcgatatccc cgcgctcgat cacagtgacg cgcatgccgc gcagcgccag gtcgtgcgcc
     5041 gtcgccgagc cggtagagcc ggccccaatg atgataatgt gaggtgctga catcgagtaa
     5101 acccgcttac tagagcgcat ccattgcgta gtgtacatat agcaccccgc tatcacatcg
     5161 gagtcacagt tcgtcatttc gacatctcgt accggtcaca atcttgagct tttgcaaaga
     5221 gaagttaaaa catcagccag ggctgcgcgt gtaatagccc cctatcgttc cgtgacgagc
     5281 ctgtgatgtt tagatcattc ctggtgaggt gattgtgata tggcggcggt atgcttcttt
     5341 caaaagcgag tgcatctcat gcaggatttg ctacagacag gagggccata tggaacattc
     5401 tatggactat aaaacagagc tggatcgctt gcacgcggct ggcttcacga cgcgggaaat
     5461 tgctcgtttg tatcagtttc gccggatgca tgtcgcgaac gaactggacc gggcgcctgt
     5521 cgacctggct cgcctgcgct tcgtgcgctg gctcgtcgag aacggcaggt tgacggatca
     5581 gcttgtctga tagtcgggtc gcttcacaag aggctcggga aggaatcgtt gtcgggagcg
     5641 tccgactgaa cgatgcaaag gagaacaatg atgtttacac ggattctcgt tccgctggat
     5701 gggtctgcca gagcggaaca cgctattccc gttgccgctc gtctcgcgcg aaacgctgat
     5761 gggaccgtta tgctgctggc ggtagaagcc ttccctgttg agtttca
//
LOCUS       DASZAK010000712         3313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_267646, whole genome shotgun sequence.
ACCESSION   DASZAK010000712 DASZAK010000000
VERSION     DASZAK010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3313
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267646"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..709
                     /locus_tag="VGT44_12570"
     CDS             77..709
                     /locus_tag="VGT44_12570"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2581679.1"
                     /translation="MVIGDSGHSTTTESRRQAILEAALACFASKGFTETTMEDIRKIS
                     GASTGSIYHHFSNKEMLARALYLEGRSSLNAALYASMTAHSLREGIEALVHAYLGWFE
                     QHADLGQYLLQAADSEYLSAYIKVLRQKTRTTLPTENLTEQLLQWLAPYISDGSVVRL
                     PRSLYLPLIIGPSREFVRIWLRTRQPAEIQEARDPLAKAAWLVIAAPVSQ"
     gene            899..3247
                     /locus_tag="VGT44_12575"
     CDS             899..3247
                     /locus_tag="VGT44_12575"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2581680.1"
                     /translation="MDAQQQGQQPEKRHKGLYWKSARRIIYILIAISLIFFILGAIIW
                     ILTELGFIHGDWADKLFTILTGLGAGATAFLALFGIFQFILSLIPDESKAATAPTNQS
                     PPTSIQTPSSPPASPPTQEPLYISAIVHPPVSRGIAGFPPPTNASAILQREQTVQDIY
                     NKLTQSGVSAVVLTGIGGQGKSTLAALVYRHAETQQRAGRGYFTAAPLWLRVDPAVTL
                     ADILANVCDALGVRAPDLAALSPQNQAYALFNILNSVDQPRLLVLDQFENLLDDEGRA
                     RDDRPGVGEWLDALNSDPCACRVLLTSRPDPQGTRDYPSACLLIHPLEGLSEAEGTEL
                     LRKWNVNATEAEMRAAVRRCNGHALSLTLLASLLQKRRLSLAALLNDPQLWKGNLAKK
                     LLDDIYQELSDPQRKLLAAFSIYREPVPIEAAQAIASDAALARTQSESALDVLLGQHL
                     LDAKGEGRYQPHAIVAEYARDHLVMEDQQANHQTLLAAHARAARYYQERAKTTCPPRK
                     ERRKIEDVQDLIEAVWHYCQAEQWRDAYELMEAEYIFEDVNRWGGNIILLELYQLMPL
                     NKSLLNRSQEANIYNNMGEAYYALSNYREAFSYYEQALRAYKEARDSKGEGVALINIG
                     VIYDELGQKQEALDYYERGLAISREVGNRDGEGTILNNLGRVYTALGQKQEALDYYEQ
                     ALAIGREVGDRGVEGYTLWNLGVLFYYTNVNAHCDVALACFLLARSIFEEVQSPNRDE
                     VQEWIDKLLSKVGDEQYNALLARVEPRAGEIVEQALREGGML"
BASE COUNT          789 a          970 c          912 g          642 t
ORIGIN      
        1 ttcaggaaca gggacagaga gggcagagag tggtagcgca gccgcacgta cagagagaga
       61 agaggctcgt tgcatcatgg taattggaga ttccgggcat agcacgacaa cagaatcccg
      121 gaggcaagcg atactagaag cagcgcttgc ctgttttgcg agcaagggtt tcaccgagac
      181 aaccatggaa gatatcagga agatatcggg ggccagcaca ggaagtattt atcatcactt
      241 ctcaaataaa gagatgctcg ctcgcgcgct ctacctcgaa gggcggagta gtctcaacgc
      301 ggcactttac gcctccatga ccgcgcattc cctgcgtgaa ggcattgaag cgcttgtcca
      361 tgcctatctg ggttggtttg aacaacatgc cgatctcgga caatatctcc tgcaagcagc
      421 cgattcagaa tatttgagcg cgtatatcaa agtactccgt cagaagacca gaaccacgct
      481 cccaaccgag aaccttacag aacagttgtt gcagtggctc gccccgtata tcagcgacgg
      541 gagcgtggta cgcctgccgc gctcgctcta cctcccactc attatcggac ccagccggga
      601 atttgtgcgc atctggctga ggacccggca acccgcggaa atacaagaag cacgagaccc
      661 cctcgcgaaa gcggcatggc tggtaatcgc tgcgcctgtt tcccaataaa gccagcaggg
      721 cgagccaggg cgatggccag catcgcccct accctctacc aacccgcgtg agaggctgtg
      781 gcgcgagggt aggggcgatg ctggccatcg ccctggctaa tctctataca ctgccctccc
      841 atcatgctat aatccatgcg aacacgccaa tacacaacgg cacagaggga cacgttgcat
      901 ggatgcacaa cagcagggac aacaacctga aaagaggcat aaggggcttt attggaaatc
      961 ggcgcgtcgt attatttaca tcctcattgc catatccctc atcttcttta tcctgggtgc
     1021 tattatctgg atactgacag aactgggatt cattcacggc gattgggccg ataaactgtt
     1081 tactattctc acaggacttg gtgcgggcgc tactgccttt ctcgcacttt ttggcatctt
     1141 tcagtttatt ctttcactca ttccagatga atctaaagca gcaacagctc ctactaatca
     1201 atcaccacca accagcattc agacaccttc atcgcctccc gcatcaccgc caacacagga
     1261 gcctttgtat atctctgcga ttgtacaccc gcccgtctcg cgcggcatcg ccggtttccc
     1321 accgccgacc aacgcgagcg ccatcttaca gcgtgagcaa accgtccagg atatctacaa
     1381 caaactcacc caatccggcg tcagcgccgt tgtgctgacc ggcatcggcg gccagggcaa
     1441 atctacgctg gccgccctgg tgtatcgcca tgccgaaacg cagcagcgcg ccggtcgcgg
     1501 ctacttcacc gccgcaccgc tctggctgcg cgtcgatccc gccgtcacgc tggccgacat
     1561 cctcgcgaac gtctgcgacg cgctcggcgt ccgcgcgccc gatctcgctg cgctctcgcc
     1621 acagaaccag gcgtatgctc tgttcaacat cctcaacagc gtcgatcagc cgcgcctgct
     1681 cgtcctcgac cagtttgaaa acctcctgga cgacgaaggc cgcgccaggg acgaccgccc
     1741 cggcgtaggc gaatggctcg acgcgctcaa cagcgacccc tgcgcctgcc gtgtgctgct
     1801 caccagccgt cccgaccccc aggggacgcg cgattatccc tccgcctgcc tgctcatcca
     1861 cccgctcgag ggcctcagcg aagccgaggg caccgagctg ctgcgcaaat ggaacgtgaa
     1921 tgctacagaa gcagagatgc gcgccgccgt gagacgctgt aacggtcacg cgctctccct
     1981 gacgctgctg gcctcgctcc tgcaaaagcg ccgcctctcg ctcgccgcgc tgctcaacga
     2041 cccgcagctc tggaagggca acctcgcgaa aaaactcctg gatgatatct accaggaact
     2101 cagcgacccg cagcgcaagc tgctggcggc cttctccatc taccgcgagc cagtacccat
     2161 cgaagcagca caagctattg cctccgatgc cgcgctcgca aggacccagt cggaatccgc
     2221 ccttgatgtc ttgctgggac agcatctgct cgatgcaaaa ggcgaaggcc gctaccagcc
     2281 ccacgccatc gtcgccgaat acgccagaga ccacctcgtc atggaagacc agcaggccaa
     2341 ccaccagacg ctgctggcag cccatgccag ggctgcgcgc tattaccagg aacgggcgaa
     2401 aacgacctgc cccccacgca aagagcggcg caagattgaa gatgtgcaag acttgatcga
     2461 ggcggtatgg cactattgtc aggcggagca gtggcgggat gcgtatgagt tgatggaggc
     2521 agagtatatt ttcgaggatg tcaaccgttg gggcggcaat attatcctgc tggaactcta
     2581 tcagctcatg cccttaaaca aatcactcct gaatcgttca caggaggcga atatctataa
     2641 taatatgggt gaggcatatt atgcgctgag caattataga gaagcttttt catattacga
     2701 acaagctcta cgggcataca aagaagctag agatagcaaa ggagaggggg tggcgctcat
     2761 taatattggt gtaatatatg atgagctggg gcaaaagcag gaggcgctgg actactacga
     2821 acgagggcta gccatcagcc gcgaggtggg caaccgggac ggcgaaggca caatcctcaa
     2881 caacctgggc agggtctata ctgctctggg gcagaagcag gaggcgttgg actactacga
     2941 gcaagcgctg gccatcggcc gcgaggtggg cgaccggggc gtcgaaggtt atacgttatg
     3001 gaatcttggc gtcctctttt attatacgaa tgtgaatgcg cattgtgatg tggctcttgc
     3061 ctgtttcctg ctcgccagga gtatctttga ggaggtacaa agtccgaatc gcgacgaagt
     3121 gcaggaatgg atagacaaac tgctcagcaa ggttggagac gagcaataca acgcattgct
     3181 ggcccgggtt gagccacgag caggggagat agtggagcag gcgttgaggg aaggcgggat
     3241 gttgtaaaat gggtagtgga tcgtcaattg tctaatgaca cgaaatggaa ccagggcata
     3301 tgaatgatat atg
//
LOCUS       DASZAK010000713         3015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_586881, whole genome shotgun sequence.
ACCESSION   DASZAK010000713 DASZAK010000000
VERSION     DASZAK010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586881"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /gene="ccsA"
                     /locus_tag="VGT44_12580"
     CDS             complement(<1..1052)
                     /gene="ccsA"
                     /locus_tag="VGT44_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEV2581681.1"
                     /translation="MYFSDLGYISLLLATGFAVFTMIVAVVGAVRGNLALVASAKRAV
                     LTVTFFMLLASASLIISFLVHDFGVSYVADQSSRSMPWYFTLSAFYGGQEGSLLYWAL
                     MLSVFSAIFIFSSKRAPAALVPYIMATLMAVELFFLLMLTTVSNPFVRLPVAPADGNG
                     LNPLLMDPGMLIHPPMLLMGYMSFSVPFAFAVAAMITGKLGSDWLRSIRRWTLAAWAI
                     QTAGLMLGMWWAYHVLGWGGYWGWDPVENAALLPWLTATAFLHSTMVQERRGMLKVWN
                     LGLVIASFALSIFGTFEVRSGIISSVHSFAYSSIGAYFLVFLAIIIVFSIGLFVYRLP
                     KLRPEQEFDSAISREG"
     gene            complement(1134..1583)
                     /locus_tag="VGT44_12585"
     CDS             complement(1134..1583)
                     /locus_tag="VGT44_12585"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEV2581682.1"
                     /translation="MMQTALDTSLPQEQPRRKRRLPLSFILGGLAILGAVIYLVFANT
                     QANSVYYMTVSELSNCTICKTQSVRVAGNVQTGTIVYDNAHGQVRFVINDGSRSLSVI
                     YSGVVPDIFRAGVTVVVEGRYTGQGPFQAQSLLTKCPSKFQAATPGT"
     gene            complement(1592..2050)
                     /locus_tag="VGT44_12590"
     CDS             complement(1592..2050)
                     /locus_tag="VGT44_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581683.1"
                     /translation="MAEIRLIIRAAHILAATAWVGGSLFYMLVALPALRGGAPAPGVA
                     AQMAALFKRMVNFCMGVLLLTGAYLALDRLTTTTLGWPYLVTLGIKIIAALCMFTLSI
                     YLGQSTIRRLAKRTTRLSKVAPQLTLALGIFVFALGALLNALYESAIAPN"
     gene            complement(2068..2691)
                     /locus_tag="VGT44_12595"
     CDS             complement(2068..2691)
                     /locus_tag="VGT44_12595"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEV2581684.1"
                     /translation="MDNEPITPGTGDAPVIAQRKHNAKKRNIIIFVVVSILNVGLLAL
                     LFTQLLTPASNNGATSSDPLIGQPAPNFTLSALSVSGAPAASTISLSNYKGQPVVLNV
                     WNSTCVPCIAEAPMLQAQWQKAHTQGVVFLGVDLQDVQGDGQRFLQHYGITYPNVFDA
                     DGWVAINYGVTGTPETLFINRQGVIVKRAIGQLTAQSLQSDLQLITL"
     gene            complement(2807..>3015)
                     /locus_tag="VGT44_12600"
     CDS             complement(2807..>3015)
                     /locus_tag="VGT44_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581685.1"
                     /translation="ISHVTYAPPTSLATPSLCPMLTAAGLMLALIGLMLQLAITAVGL
                     LIVIAALLWWTLGAKPDSLAIARS"
BASE COUNT          694 a          835 c          948 g          538 t
ORIGIN      
        1 ctgccttcgc gcgagatggc cgagtcgaat tcctgctccg ggcgcagctt cggcagccgg
       61 tagacgaaca ggccgatgct gaagacgatg atgatggcca ggaagacaag gaagtacgcg
      121 ccgatagagg aataggcgaa ggaatgcacc gaactgatga tgccgctgcg cacctcgaag
      181 gtgccgaaaa tggagagggc gaaggaggcg atcaccagcc ccaggttcca gaccttgagc
      241 attccgcgcc gctcctgcac catcgtggag tgcaggaagg ctgttgccgt cagccagggc
      301 agcagcgcgg cgttctccac cgggtcccag ccccaatagc cgccccagcc aagcacgtga
      361 taggcccacc acatgccgag catcagcccc gccgtctgaa tagcccaggc cgccagcgtc
      421 cagcgccgga tggagcgcag ccaatcgctg cccagtttgc cggtaatcat agcggcgacg
      481 gcaaaggcaa agggaacgga gaagctcata tagcccatta ggagcatggg cgggtggatc
      541 aacatgccgg ggtccatcag gagcgggttg aggccgttgc cgtcggcagg cgcgaccggc
      601 aggcgcacga agggattgga gacggtggtc agcatcagta aaaagaagag ttcgaccgcc
      661 atcagcgttg ccatgatata tggtacaagc gcggcgggag cgcgtttcga gctaaaaatg
      721 aagatggcgg agaagaccga gagcatcaag gcccagtaga gcaacgaacc ctcctgccca
      781 ccatagaagg ctgagagggt gaagtaccag ggcatggagc ggctggactg gtcggccaca
      841 tagctgacgc cgaaatcgtg taccaggaac gagatgatga gcgaggctga ggccagcagc
      901 atgaagaagg tgacggtcag cacggcgcgt ttcgcgctgg caaccagggc gagattgccg
      961 cgcacggctc ccacaaccgc gacgatcatg gtgaagacgg cgaagccggt cgcgagtaat
     1021 aatgaaatgt agccaagatc agaaaaatac acggaatgtc cttactgagc ttccctttgc
     1081 atggcgaccc tcgcggtcgc catgcaacaa cacgatggtt gctcttatgc ttgctaagtg
     1141 ccgggcgtcg ccgcctggaa cttcgatggg catttggtca gcaggctttg tgcctggaat
     1201 ggcccctggc cggtgtaacg cccctcgacc acgacggtca cacccgcgcg gaaaatatcg
     1261 ggtacaaccc cgctgtagat caccgatagg gagcggctgc catcgttgat gacgaagcgc
     1321 acctgtccat gcgcattgtc gtagacgatg gttccggtct gcacattgcc tgcgacgcgc
     1381 accgattgcg tcttgcagat ggtgcagttc gacaattcgg aaacggtcat gtagtagacc
     1441 gagttggcct gggtgttggc aaacacaagg tagatcaccg cgccaagaat ggccagaccg
     1501 cccaggatga agctaagcgg caggcggcgc ttgcggcgcg gttgttcctg cggcagcgat
     1561 gtatcgagag cggtttgcat catgcatcct ttcaattggg cgcgatggcg gattcgtata
     1621 gagcgttgag caaagcgccg agcgcaaata caaatatgcc cagggccagc gtcagttgtg
     1681 gcgcgacctt cgagaggcgc gtcgtgcgct tcgccagccg tcgtatagtg ctttgaccaa
     1741 gatagatcga tagggtaaac atgcagagcg cggcgatgat cttaatgccc agcgtcacca
     1801 ggtagggcca gcccagcgtc gtcgtggtca ggcggtcaag cgccaggtac gcgcccgtca
     1861 gcagcaagac tcccatgcag aaattgacca tgcgcttgaa gagcgctgcc atctgcgccg
     1921 ctacgccggg agcgggcgcg ccgccgcgca gcgcgggcaa ggctaccagc atgtagaaca
     1981 ggctgccgcc tacccaggca gtcgcggcca gaatatgtgc cgctcgaata atcaaacgta
     2041 tctctgccat gatcggctgt gccgcttcta gagcgtaatc aactgcaggt cgctctggag
     2101 tgattgcgcg gtcaactgtc cgatggcgcg cttgacaatc acgccctgcc ggttaataaa
     2161 gagtgtttcc ggtgtgccag tcacgccata attgatagcg acccagccgt cggcatcaaa
     2221 gacattcgga taagtaatgc cgtaatgctg caaaaaacgc tgcccgtcac cctggacatc
     2281 ctgcaaatca acaccgagaa agaccacgcc ctgcgtgtgt gccttctgcc attgcgcttg
     2341 caacatgggc gcttcggcga tgcatggcac acaggttgaa ttccacacgt tcagcactac
     2401 cggctggcct ttgtagttcg atagagaaat ggtggacgcc gctggcgcgc cggatacgct
     2461 cagcgcggaa agcgtaaaat tgggcgcggg ctgtccgatc aaagggtcag acgatgttgc
     2521 cccattattt gacgcgggcg tcagcaattg cgtgaacaac agggcgagca gccccacgtt
     2581 caagatgctg accaccacga aaataatgat gttgcgcttt ttggcgttat gcttccgctg
     2641 cgctatgaca ggcgcatcgc cagtgccagg cgtaatcggt tcgttgtcca ttgtcgcgtc
     2701 catacaaaaa taaatagaag acaccagtgt tgattattgg atgtgggact gatagagggc
     2761 ttgcaacctg tggagtagac cttacaggca cctgaatgtt cgccgcttag cttctggcga
     2821 tggcgaggct gtcaggcttt gcgccgagcg tccaccacag cagggcggca atcacgatta
     2881 gcagaccaac ggcggtaatg gcgagttgca gcatcaggcc aatcagggcc agcatcagcc
     2941 cggcggcggt cagcatggga cagaggcttg gcgtggcgag cgaggtcggc ggcgcgtagg
     3001 tgacatgaga gatcg
//
LOCUS       DASZAK010000714         3116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_160802, whole genome shotgun sequence.
ACCESSION   DASZAK010000714 DASZAK010000000
VERSION     DASZAK010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160802"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="VGT44_12605"
     CDS             <1..1279
                     /locus_tag="VGT44_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase S53"
                     /protein_id="HEV2581686.1"
                     /translation="NPDPIQTAQVQVRYLFQGSTGPAITHDVAPQSRYTINPNGDLHI
                     GQNSNPGKAISMIVTSVNNVGIVAERPMYFTWRGISSGTDTLGTTKLGQDFYFADVEA
                     QRNYSSFITILNPPGGKTANVSLSYVAGGAQVGTQTLVVPSGQRATTWPKIAGINQQS
                     ALHVHSDQPVIVERPMYFTTSRSNISGAVTGAATIVGAPAPNADWLFAEGYTGTNFHE
                     YLVLANFDATVTANVTVKLEYSNGAVNPTVVQVPPLSQYVFDVNAASAAFAQSTPELS
                     AEVTSDAPIVAQRQEYFRFNGAIPGGTDVIGEPGPAQTIYSFAEGYTASGFNEFLTLQ
                     NPNTTSESVAVTLYLAHSIIAQQVVTVGPQTRVTVNINSLVTPIAQSNPAAGYEVSLA
                     VWTSSGTIVAERPMYFDYHNAAWGGTDVVGFTG"
     gene            complement(1286..2911)
                     /locus_tag="VGT44_12610"
     CDS             complement(1286..2911)
                     /locus_tag="VGT44_12610"
                     /inference="COORDINATES: protein motif:HMM:NF045017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581687.1"
                     /translation="MAKQPGNLSKFGRLGSFKGLVTLGVSIMLVVVMAWGVVTGRFGG
                     VLAGNFSQSTNGAVQLAPPVSPYLFGTNLGLFNPNDQVLTSTTTRSILQQMHVRIIRM
                     PLRTGLAESVEVQAAQTIKSMGAVPLVVLHGAVDATVLADDSLVISDMNIVFGHSLVY
                     YEYGNEEDLLGVDANGYTASWNAIVPQLKHLALNGQFIGPVNFQYDGPYLTTFLQNAN
                     PRPDEVSWHEYTCDDSWANSICISHIANWTNHINAARAAMTGAIGTALPIMITEWNYA
                     PNAVPNDGKNNNSAFMATWTASALQTLAANGIFASMQYSVTNTAIPMVDGNNTATIQG
                     TTFASQYQAMINGGQRPPVPTPGQPQPTSPAGGYGNGSGGVTPQPTTQPIVTNQYSSF
                     TFEDGGIDGWSGRGEVSQVLNSSSVSMSGGKHSLQVTLTNMGQGDYPFATCGGSYLSS
                     YPKAGQTITAYLYLPANSSNIMAKLFVMDSQFHWFDPGDMVTLIPGTWNRLTFSLPAN
                     VSTGQMRQIGVQFNTGDTNPVSGTVYIDNIGWS"
BASE COUNT          636 a          889 c          942 g          649 t
ORIGIN      
        1 gaatcctgac cctattcaga cggcgcaggt gcaggtgcgc tatctcttcc agggcagcac
       61 cggcccggcc attacgcacg acgtagcgcc acagagccgc tataccatca accccaatgg
      121 cgacctgcat atcgggcaga acagcaatcc gggcaaggcc atctcgatga tcgtcacctc
      181 ggtcaacaac gtgggtatcg tcgcagagcg gccgatgtac ttcacgtggc gcggcatcag
      241 cagcggcacg gatactctgg gaacaacgaa gctggggcag gacttctatt tcgccgatgt
      301 cgaggctcag cgcaactatt ccagcttcat caccatcctc aacccgcccg gcggcaagac
      361 cgccaatgtg tcgctgagct acgttgccgg cggggcgcag gttggcacgc agacgctcgt
      421 cgttccatcc ggccagcgcg ctaccacctg gccgaagatt gccggtatca accagcagag
      481 cgcgctgcat gtgcattccg atcagccggt catcgtggag cgaccgatgt acttcacgac
      541 ctcgcgctca aatattagcg gagcggtgac gggcgcggcc accatcgtcg gcgcgccggc
      601 gcccaacgcc gactggctgt ttgccgaagg ctataccggc acgaatttcc atgaatatct
      661 tgtgctggcg aattttgacg cgacggtcac ggccaatgtc accgtcaagc tggaatatag
      721 caatggcgcg gtgaacccga cagtcgtgca ggtgccgccc ctttcgcaat atgtcttcga
      781 tgtgaacgcg gcctcggctg cttttgcgca gagtacgccc gaactgtcgg ctgaagtcac
      841 ttcggatgcg ccgattgtgg cgcagcgtca ggagtacttc cgcttcaacg gcgctatccc
      901 cggcggcacc gacgtgatcg gcgaaccagg cccggcgcag accatctaca gcttcgccga
      961 gggctatacc gccagcggct tcaacgagtt cctgacgctc cagaatccca atacgacgag
     1021 tgaaagtgtg gccgtgacgc tctacctggc gcacagtatc atcgcccagc aggttgtgac
     1081 cgtcgggccg cagacgcgcg tcacggtcaa catcaatagc cttgttacgc ctatcgcgca
     1141 atccaatccc gctgcaggtt atgaagtctc gctggcggtc tggacctcca gcggcaccat
     1201 cgtcgctgaa cgcccgatgt acttcgatta ccacaatgcc gcctggggcg ggacggatgt
     1261 cgtcgggttc acgggatagg cagcgttaac tccacccgat attatcgatg tagaccgtcc
     1321 cgcttaccgg gttggtgtca ccggtgttga actgtacgcc gatctggcgc atctggccgg
     1381 tgctgacatt ggctgggagc gagaacgtca ggcgattcca cgtgccgggg atcaatgtga
     1441 ccatgtcgcc gggatcaaac cagtgaaact ggctgtccat aacgaataac ttggccataa
     1501 tgttggacga gttggcgggc aggtagaggt aggccgttat ggtctgcccg gctttcgggt
     1561 aactggacag gtaggagccg ccgcaggtcg cgaaggggta gtcaccctgg cccatgttgg
     1621 taagcgttac ctgaagcgag tgtttcccgc cgctcatgct gacgctgctg ctgttcagca
     1681 cctgtgagac ctcgccgcgt cccgaccatc catcgatgcc gccgtcctcg aaggtgaatg
     1741 aggagtactg gttggtcact atgggttgag tggtaggctg aggcgtcact ccgccactgc
     1801 cattgccgta tccgccggcg ggcgacgtgg gctgtggctg accaggagtt gggacaggtg
     1861 gccgctggcc gccgttgatc atagcctggt actggctcgc gaaggtggtt ccctgaatag
     1921 ttgcggtgtt gttgccgtcg accatcggga tagcagtatt ggtgacggaa tactgcatcg
     1981 aggcgaagat gccgttggcg gcaagtgttt gcaacgcgga ggccgtccag gtggccatga
     2041 aggcgctatt gttgtttttg ccatcgttgg gaacggcgtt cggagcgtag ttccactcgg
     2101 taatcataat cggcagcgcg gtgccaatcg cgccggtcat ggcggcgcgc gcggcgttga
     2161 tatggttggt ccagttggcg atatgcgaga tgcagatgga gtttgcccac gaatcgtcgc
     2221 aggtatattc atgccagctc acttcgtctg gccgggggtt agcgttttgc aggaaggttg
     2281 tgaggtaggg tccatcgtac tggaaattga ccgggcctat aaattgcccg ttcagcgcga
     2341 gatgcttgag ttgtggcaca atcgcgttcc acgacgcggt gtagccgttg gcatctactc
     2401 ccagcaggtc ctcttcgttg ccgtactcgt agtaaaccag gctgtggccg aaaacgatgt
     2461 tcatgtcgga gataaccagc gagtcgtcgg ccagcacggt cgcatcgacg gctccgtgca
     2521 atactaccag tggcactgcg cccatcgact tgatggtctg cgccgcctgc acttcaaccg
     2581 attcggcgag gccggtgcgc agcggcatac gaataatgcg cacgtgcatc tgctgcaaga
     2641 tcgagcgcgt cgtagtagaa gtcagtacct ggtcgttggg attgaataat cccaggttgg
     2701 tgccaaatag atagggcgaa acaggtggag cgagttgtac cgcgccgttc gtcgattgcg
     2761 agaaattgcc ggcgagtacc ccgccgaatc tgccggtgac gacgccccac gccatcacga
     2821 cgaccagcat aatcgagacg cccagcgtca caagcccttt gaaactgccc agtcttccaa
     2881 acttactcaa gttccctggc tgttttgcca tgccctactc tccttgtggt tacaagtgac
     2941 agtacacaat gaagatcaat ggtacatgtt catatacgaa ggtaccccgt gctgaatata
     3001 gcatcataga taaacacgac aagaacagtt tcgcggaaaa tggtaccttt gcgctatgca
     3061 ggggttaagg tagaatggac aagagggcga ccgcaagggt gcgcccttac aatgac
//
LOCUS       DASZAK010000715         2579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_374318, whole genome shotgun sequence.
ACCESSION   DASZAK010000715 DASZAK010000000
VERSION     DASZAK010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374318"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..1470
                     /locus_tag="VGT44_12615"
     CDS             289..1470
                     /locus_tag="VGT44_12615"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV2581688.1"
                     /translation="MRILIAGGDGYLGWPQALYLSGQGHEITLFDNLMRRHFDLQQGY
                     DSLTPISSIHERIAAWKEISGQQIQFVVGDTVDYEALSGVFRDFQPQAVVHFAEQRSA
                     PYSMIDRKHAVFTQTNNVIGTLNLLYVIKEFAPDCHLVKLGTMGEYGTPNIDIEEGFI
                     EIHHKGRSDVLPFPKQPSSFYHLSKVHDSHNIMFCCKIWGLRATDLNQGIVYGVETDE
                     TAQDVRLATRFDYDQIYGTALNRFCVQAAAGYPLTVYGNGQQIRGYINLRDTIRCIEL
                     AINTPPAEGEYRVFNQITEQFSLSELADMVVKQSRYMGLEARIGHLANPRVEAEQHYY
                     NATHTRLIDLGLEPHFLNEAVLQSLIGLAIQHRARIEHSLILPTVSWRMTRNEVTPTD
                     S"
     gene            complement(1465..2559)
                     /locus_tag="VGT44_12620"
     CDS             complement(1465..2559)
                     /locus_tag="VGT44_12620"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2581689.1"
                     /translation="MARPTILILTTHTGGGHLNLARSLRDMLEPDYDVVIANPQPGIF
                     GPAYTAQSRHAPKLIELQHTLTDSARAAFLYHRLLALFSSGHILALLERYRPHLVITT
                     HALLSYATARVIERSRRHTLLVFQLTDLERLHMTWFTEKQADAYFAPTKEIFVQAQQE
                     GIDEDRLHLTGRPVRRQFGEVSPEQTQQIRTGLGFDATTFTIFLQGGATGSSGIDGLI
                     NGLLSINMPVQVILAAGNNASMASRYAGIEQVRSLPFTERLAPYMAIADMIAGKAGAS
                     FISEAFMLEKPFLATTFISGQETPNLHFIEQHNLGWVCLKTQNQLELLSSLARHPEML
                     AAKLPSIRDYKAWNSEANRRFRPVIDRLLS"
BASE COUNT          577 a          689 c          706 g          607 t
ORIGIN      
        1 agtgacttga gtcaaataaa tgttgatcga ggttcatgca ggtcgagtat tttacaaata
       61 ttttacaatt ttctgccttt ttccagagag ttctactata cgctgagttg aacgctcttg
      121 cggaaaaacc ggggaatgat aacccgttgt aggggcgcaa ttcattgcgc cctcataccc
      181 gaccaggcgc atccgcaggg cgcaatgaat tgcgccccta caacgggttc attcacccaa
      241 aaccagggcg agattggcag gtcaatcaaa tattatgagg caaagattat gcgcattctt
      301 attgccggcg gcgacggcta tcttggctgg ccacaggctc tctacctgtc cggccaggga
      361 catgaaatta ctctattcga taatctgatg cgaaggcact ttgatctcca acagggatat
      421 gatagcctga cgccaatctc ttctatccac gagcggatcg ccgcgtggaa ggagatctcc
      481 gggcagcaaa tacagtttgt tgttggcgat acagtggatt atgaagcgct ttccggagtc
      541 tttcgcgact ttcagccgca agcggtggtg cattttgccg agcagcgatc cgcgccctat
      601 tctatgattg accgcaagca tgccgttttt acgcagacga acaacgtgat tggcacactc
      661 aacctgttat acgtcatcaa ggaattcgcg ccggattgcc atctcgtgaa gctgggaacg
      721 atgggtgaat atggcactcc caacattgat atcgaagagg gttttatcga aattcatcac
      781 aaaggccgca gcgatgtgct gcccttcccg aaacagcctt cctcttttta tcaccttagt
      841 aaggtccacg acagtcacaa tatcatgttt tgctgcaaaa tctgggggct gcgggcgacc
      901 gatttgaatc aaggcatcgt ctacggcgtt gaaaccgatg aaacggccca ggatgttcgc
      961 ctggcaaccc ggttcgatta cgatcagatt tacggcacgg ccctgaatcg tttctgcgtc
     1021 caggctgccg ccgggtatcc tctcactgtc tatggcaatg ggcagcagat tcgcggttat
     1081 atcaatttgc gggataccat tcgatgcatt gaactggcga tcaatacgcc gcccgccgaa
     1141 ggtgaatacc gcgtcttcaa tcagatcacc gagcaatttt cgttgtccga actggccgac
     1201 atggtcgtca aacaaagccg atacatgggg ttggaggcgc gcatcgggca tcttgccaac
     1261 ccgagagtgg aagctgagca gcactattac aacgctaccc atacccggct gatcgacctg
     1321 ggactcgaac cgcatttcct gaatgaagct gttctccaat cactgatcgg cctggctatc
     1381 cagcaccgcg ctcgcatcga acatagcctg attttgccga ctgtgagctg gcgcatgact
     1441 agaaacgagg tcacgccgac cgattcatga gagcagccgg tcaataaccg ggcgaaagcg
     1501 ccgatttgcc tcgctattcc aggctttgta gtcgcgaatg ctgggcagct ttgcggcgag
     1561 catctcagga tggcgcgcca ggctcgatag cagctcgagc tggttctgcg ttttcaggca
     1621 tacccagccg aggttgtgct gctcaatgaa atgtagattg ggggtctcct ggccggagat
     1681 aaaggttgtt gcgagaaacg gtttttcgag catgaatgcc tcgctgataa agcttgctcc
     1741 ggccttgccc gcaatcatat ccgcgatagc catgtaggga gcgagccgtt cggtgaacgg
     1801 caggcttcgc acctgttcaa tgcccgcgta acgcgatgcc atgctcgcat tgttaccggc
     1861 ggccaggatg acctggaccg gcatatttat gcttagcagc ccattgatga ggccgtcgat
     1921 tccggatgag ccggtcgcgc ctccctggag aaaaatggtg aaggttgtcg cgtcgaaccc
     1981 caacccggtg cgaatctgtt gcgtttgctc cggcgagacc tccccaaact ggcgtctcac
     2041 tggcctgcct gtcaggtgta aacggtcttc atcgatgccc tcttgctgtg cctggacgaa
     2101 gatttccttt gtcggcgcaa aataggcatc ggcctgcttt tcggtgaacc aggtcatgtg
     2161 cagccgttcc aggtcggtca gttggaagac cagcagcgta tgcctccggc tgcgctcaat
     2221 cacccgcgca gtcgcgtaag aaagcagcgc gtgtgtcgta atcaccaggt ggggccggta
     2281 gcgctcaagc aaggcgagga tatgtccgct actgaacagc gcaagtagcc ggtgatagag
     2341 aaatgcggct cttgcgctat cggtcaatgt atgttgtagc tcgatcagtt tcggagcatg
     2401 gcggctttgg gcggtgtagg ctggaccaaa tatgcccggc tgagggttcg ctatgacgac
     2461 atcgtagtca ggctccagca tatctctcaa cgagcgcgcc aggttgagat gcccaccacc
     2521 ggtatgagtc gtcagtataa gaatggtcgg ccttgccatg cccgtcaata tcccctact
//
LOCUS       DASZAK010000716         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_52890, whole genome shotgun sequence.
ACCESSION   DASZAK010000716 DASZAK010000000
VERSION     DASZAK010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52890"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VGT44_12625"
     CDS             <1..219
                     /locus_tag="VGT44_12625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581690.1"
                     /translation="DTITSDNFTFTILATRGRRITKVRVERVRQQEQPQPIRLLPSPS
                     SNASNEKNKSDEQYTSDGERSHIESRGA"
     gene            223..1149
                     /locus_tag="VGT44_12630"
     CDS             223..1149
                     /locus_tag="VGT44_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEV2581691.1"
                     /translation="MATPHQFAHLPTTVRVVEVGPRDGLQNEKALVPTPQKVQFIEML
                     ALAGLPVVEATSFVSPRAIPQLSDAGEVMSRLTRLPSTVYPVLVPNVRGMERALAAGV
                     RSIAVFTAASESFTRHNINATIAESLDNFRPVVAMARQVGIPVRGYISTVFGCPYEGK
                     VDPRSVLHVAQELLDMGINELSLGDTIGVATPNQVIDVLGLFLEEGAIPVEQIAVHFH
                     DTRGTALANVLAALQLGVSIVDASAGGLGGCPYAPGAAGNLATEDLLYMLHGMGIQTG
                     VDLAKVVEATGFIAPYLGHAPMSKYYQAATGG"
     gene            complement(1309..>2385)
                     /locus_tag="VGT44_12635"
     CDS             complement(1309..>2385)
                     /locus_tag="VGT44_12635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEV2581692.1"
                     /translation="QNYVDRKAINYQEMSYDAIRAMLADLHDKGHTYFLSPDELKAQQ
                     QELSGSSSGIGIYIQQDPKTNNIIIEATVPGAPADKAGFKSGDIIKAVDGTSTAGKSM
                     DAVHALIDGKVGTTVAITVLRPSTGQTLTIKVQRANFTIPNVILHYIAETHIAHIQVL
                     GFDNGVSDQLKTALIQAKKDGATGIILDLRDNPGGYLTEAINTASLFLSGGKTVLLEQ
                     DSTGNRTADKTTGSPVDTQIPLVVLVNGNTASAAEIVSGALQDNKRATIIGTTTLGTG
                     TVLNEFDLPDGSAIFLGVMEWLTPDGHFIRDKGITPNVVVTLPANNNALTPNVENQQN
                     LTLQQILQSGDTQLINAIKNLQGH"
BASE COUNT          439 a          708 c          727 g          511 t
ORIGIN      
        1 gatacgatca cgtccgacaa cttcaccttc accatcctgg ccacgcgcgg caggcgcatc
       61 acgaaggtgc gcgtcgagcg tgtccgccag caggagcaac cccagccgat ccgtctgctc
      121 ccttccccat catcgaatgc atccaacgag aagaacaaga gcgatgagca gtacacctcc
      181 gatggagagc gctcccatat cgaatcgcgg ggggcataat cgatggccac gccgcaccag
      241 tttgcccatc tgccaacaac tgtgcgcgta gtagaggtgg ggccgcgtga tggattgcag
      301 aacgaaaaag cgctcgtgcc gactccgcag aaggtacagt tcatcgaaat gctggcactg
      361 gccgggctgc cggtcgtcga agccacgtcc ttcgtcagcc cgcgcgctat tccgcagttg
      421 agcgatgcag gcgaggtgat gagcaggctg acacgcctgc cgtccaccgt ctatcctgta
      481 ctggtgccga atgtcagggg catggaacgc gccctggccg ccggggtgcg ttccatcgcc
      541 gtcttcaccg ccgccagcga gagtttcacg cgccacaata tcaacgccac catcgcagaa
      601 agcctcgaca actttcgccc ggtcgtggcc atggcgcggc aggtcggcat tcctgtgcgc
      661 ggctacatct ccaccgtgtt tggctgcccc tacgagggca aggtcgatcc tcgcagtgtc
      721 ctgcatgtcg cccaggaact gctcgacatg ggcatcaacg aactctcgct gggcgatacc
      781 atcggcgtcg ccacgcccaa ccaggtcatc gatgtcctcg gcctttttct tgaagagggc
      841 gccatccccg tcgagcaaat cgccgtccac tttcacgata cgcgcggcac ggcgctggcg
      901 aatgtgctgg ccgctctgca actcggcgtc tccatcgtcg atgcctcggc tggcggcctg
      961 ggcggctgcc cctatgcccc tggcgcggcg ggcaatctcg ctaccgaaga cctgctctac
     1021 atgctgcacg gcatgggcat ccagaccggc gttgacctgg caaaagtggt cgaggcgaca
     1081 ggctttattg cgccatacct cggacatgcg ccgatgagca agtattacca ggcggctact
     1141 ggagggtaac gcccctggga tgatgcattg tcgagaacca atcgcgagag gcatggcgac
     1201 cgcaagggtg cgccactccg ataccgcctg gagggcgaac gtcgcctcgt tatcgtagtg
     1261 gcgcaccctt gcggtcgcca tgcctctcgc gactttgcat aggccctgct aatgcccctg
     1321 caagttcttg atcgcgttaa tcaactgcgt atccccgctc tggagaatct gctggagggt
     1381 cagattctgc tggttctcca cgttcggggt tagcgcatta ttgtttgcgg gcagggtgac
     1441 gacgacgttg ggtgtgatgc ctttatcgcg gatgaaatgg ccgtcgggcg tcaaccattc
     1501 catcacgccc aggaagatgg cggagccgtc gggcaagtcg aattcgttga ggacggtgcc
     1561 tgtgccgagt gtggtggtgc ctatgatggt ggcgcgcttg ttgtcctgca acgcgccgga
     1621 gacgatttcc gcggcgctgg cggtgttgcc gttcaccagc acgaccagcg ggatctgtgt
     1681 atcgacgggc gagccggtcg tcttatcagc cgtgcgattg ccggtgctat cctgttcgag
     1741 cagcaccgtt ttaccgccgc tcaggaacag gctggcggtg ttgatggctt ccgtgagata
     1801 gccgccgggg ttgtcgcgca ggtcgagaat gatacccgtt gcgccgtcct tcttggcctg
     1861 gatcaaggcg gtcttgagtt gatccgagac gccgttgtcg aaaccgagga cctgaatgtg
     1921 cgcaatatgc gtctcggcaa tgtagtgcag gatgacgtta gggatggtaa aattggcgcg
     1981 ctgcactttg atcgtgaggg tttgtccggt agaaggacgc agcacggtaa tagcgacggt
     2041 cgtgccaacc ttcccatcga tcagcgcgtg tacggcgtcc atgctcttac ctgccgtact
     2101 cgtgccatcg actgctttga tgatatcacc actcttgaag ccggctttat ccgccggcgc
     2161 gcccggcacc gttgcctcaa tgataatgtt attcgttttg gggtcttgct ggatgtagat
     2221 gccgatgcct gaagaagaac cactcagttc ttgctgctgc gccttcaatt cgtcgggaga
     2281 caggaagtag gtgtggcctt tgtcgtgtag atcggccagc atagcgcgga tagcatcata
     2341 cgacatctcc tggtagttga tggctttgcg atcgacatag ttttg
//
LOCUS       DASZAK010000717         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_479889, whole genome shotgun sequence.
ACCESSION   DASZAK010000717 DASZAK010000000
VERSION     DASZAK010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479889"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..994)
                     /gene="pstB"
                     /locus_tag="VGT44_12640"
     CDS             complement(125..994)
                     /gene="pstB"
                     /locus_tag="VGT44_12640"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEV2581693.1"
                     /translation="MDTHLNKLKQEQAVEESVAKPVPSIPRVVTEAAPQANIDASIKI
                     DVRNTNFWYSAKQALYDVTIPIRDKQVTALIGPSGCGKSTFLRTLNRMNDLIPGTRIE
                     GEALLDGENIYDRTVDVVSLRQRVGMVFQRANPFPKSIYENVAYGLRAQGKSGRFVQE
                     RVERSLRGAALWDEVKDRLKASALGLSGGQQQRLCIARAIAVNPDVLLMDEPASALDP
                     IATLKIEELIVELVKDYTIVIVTHNMQQAARVSQFTGFFLSGHLIEYAPTVDLFEHPK
                     QKETEDYISGRFG"
     gene            complement(1125..1358)
                     /locus_tag="VGT44_12645"
     CDS             complement(1125..1358)
                     /locus_tag="VGT44_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581694.1"
                     /translation="MNLSADIRTPDGGGNSSLPEGANIFSRKVARSGHISYEGRPYFI
                     SKALAGRYIRLVVIADRMIVDAAIPLHKEYSLS"
     gene            complement(1413..2165)
                     /gene="pstB"
                     /locus_tag="VGT44_12650"
     CDS             complement(1413..2165)
                     /gene="pstB"
                     /locus_tag="VGT44_12650"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEV2581695.1"
                     /translation="MSVQIRNLNVWYGQAQALHDVNLTIESQGATAIIGPSGCGKSTF
                     VRCLNRMHEEVHGGRVKGEVLIDDINIYARSIKPMYVRRHIGMVFQRPNPLPALSIFD
                     NVAIGLRLNGLASGSALKEIVERSLKQAALWDEVKDRLGESASQLSGGQQQRLCIART
                     LVIEPKVLLMDEPCSALDPISTFQVEELIRELKQNYTVVIVTHNMQQAGRVADRTVFF
                     LQGKLIEEGETAGFFAHPQKKETEDYISGRFG"
     gene            complement(2221..3129)
                     /gene="pstA"
                     /locus_tag="VGT44_12655"
     CDS             complement(2221..3129)
                     /gene="pstA"
                     /locus_tag="VGT44_12655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV2581696.1"
                     /translation="MNTTTVSEKQSVENPFQPPRTAHNARRRISNIIIITFSLLATAF
                     AVCVLLFLLLYVLRAGLPYINLDLFTKIPSPNGEPGGGLGPAIQGSLILVGLAALFGV
                     PLGLFTGIYLSEFGGRGFISTGVRFLVDVLTGIPTIIFGLFAWVFVVVPQQSFSGLAG
                     GVALGIIMIPIVARTSEEVLRLVPKELREASLALGATESRTILRVVIPAASSGIITGI
                     ILAMARVAGETAPLLMTAFGNPFFNSDITQPMDALPLRIFNFAESPYQGQINQAYAGS
                     LVLIVLILLTSFAIRWATGGFRRNGR"
BASE COUNT          687 a          901 c          865 g          703 t
ORIGIN      
        1 gacctggaac ccggcctgtt ccaggttgta ggcgatggtc tccaccagca ccggctcatc
       61 atcgacaacc aggatttttc ttcccacgcc ttgcctccct atgtgaccag ggcgacctgg
      121 catcttaacc aaatcgccca ctgatataat cttcggtttc tttctgtttg gggtgttcaa
      181 agagatcgac tgtcggcgcg tactcgatca ggtgtccgga gaggaagaag ccggtaaact
      241 gcgagacgcg cgccgcctgc tgcatattat gtgtgacgat cacgatggtg tagtccttca
      301 ccaactcgac gatcagttct tctatcttca gtgtagcaat ggggtcaagc gcggaggccg
      361 gctcgtccat cagcagcaca tcggggttga ccgcgatggc gcgcgcgatg cacaggcgct
      421 gctgctgccc gccggagaga cccagggcag aggctttgag gcgatccttc acctcatccc
      481 acagcgcagc cccgcgcagg ctgcgttcga cgcgctcctg aacgaacctg cccgatttcc
      541 cctgcgcgcg caggccatac gccacattct cgtaaatcga tttagggaac ggatttgctc
      601 gctgaaagac catgccgaca cgctggcgca gcgaaaccac atcgaccgtc ctatcgtaga
      661 tattctcacc atccaaaagc gcctcgccct cgatgcgcgt tccaggaatc agatcgttca
      721 tacggttcag tgtgcgtaga aacgtcgatt tgccgcatcc cgatgggccg atcagcgccg
      781 tcacctgttt atcgcggatg gggatcgtca catcataaag cgcctgcttc gcgctgtacc
      841 agaagttcgt gttgcgcaca tcgatcttga tcgaggcatc aatattggcc tgcggcgcgg
      901 cttccgtcac caccctgggt atgctgggaa ccggttttgc gacgctttct tccaccgctt
      961 gctcctgttt caatttattg agatgtgtat ccatcaatac tcctcgcccc tgtcgtgggg
     1021 ttgtgatgat gaagtttaaa tagtaaaaag cggggacaca tctccgtagg tgccccatta
     1081 attatttaat gttcatcatc gcgtccccac gactctatta tatgttatga cagagagtat
     1141 tctttgtgca aagggatggc ggcatcgacg atcatgcggt cggcgatcac taccaggcgg
     1201 atgtagcggc ccgccagggc tttgctaatg aaataggggc gtccttcata cgaaatatgt
     1261 ccactacgcg cgactttacg agagaagatg tttgcccctt cgggaagcga gctatttcct
     1321 cccccatccg gggttctgat atctgctgat aaattcatcg tggtagtatc ctcgtttctt
     1381 ccgttaagtg accttgatat actatgacgc tctcaaccga agcgtcctga gatgtagtct
     1441 tcggtttctt tcttctgggg atgcgcgaag aatcctgcgg tctctccctc ttcgatgagt
     1501 ttcccctgga gaaagaagac ggttctatcc gccacgcgcc cggcctgctg catgttatgc
     1561 gtcacgatca caacggtgta gttctgcttg agttctctaa tcaattcttc gacctgaaag
     1621 gttgatatgg gatcgagcgc tgaacagggt tcgtccatga gcagcacttt tggttcaatg
     1681 accagtgtgc gggcaatgca caatcgctgt tgctggccgc ccgacaactg cgaggcggac
     1741 tcacccaacc ggtctttcac ctcatcccag agcgcggcct gcttgagact gcgctcaacg
     1801 atctctttga gcgctgagcc acttgccagt ccattcaggc gcaggccgat tgccacattg
     1861 tcaaagatgc taagtgccgg cagtgggttc gggcgctgaa acaccatgcc gatatgacgc
     1921 cgcacgtaca tgggcttgat tgaacgcgcg tagatgttga tatcatcgat cagcacctca
     1981 cctttcacgc ggccaccatg cacttcctcg tgcatgcgat tcaggcagcg cacgaaggtc
     2041 gatttgccgc accccgacgg gccaatgata gcggtagcac cctgcgattc gatggtgagg
     2101 ttcacatcgt ggagcgcctg tgcttgcccg taccacacgt tgagattacg tatctgtacc
     2161 gacacgcctt cctccttcta tacctggggc atactatgcg ccttgatcga acgtccatgt
     2221 ttagcgacca tttcggcgaa accctcctgt agcccagcgt atcgcaaacg aagtcaggag
     2281 gatgagaacg atgagaacga gcgatcccgc gtatgcctga ttgatctgtc cctgataggg
     2341 gctttccgcg aagttgaaga tgcgcagggg cagggcatcc ataggctggg tgatatccga
     2401 gttaaagaat gggttgccga aggcggtcat caaaagcgga gcagtttcac cggccacgcg
     2461 cgccatggcc aggatgatgc cggtgataat gccgctgctc gccgccggaa tgacgacgcg
     2521 caggatggtg cgggactcgg ttgcgccgag ggcaagcgag gcttcgcgca gttctttcgg
     2581 caccaggcgt aagacctctt cagaggtgcg agccacaatc gggatcatga taattcctag
     2641 cgccacgcca cccgccaggc ccgaaaacga ttgctgcggc accacaacaa aaacccaggc
     2701 aaacaggcca aagatgatgg tcggtatgcc ggtcagcaca tcgaccagaa agcgaacccc
     2761 ggtggagatg aacccacgcc ctccgaattc agagaggtag atgccggtga acaggccaag
     2821 tgggacgccg aagagcgctg ccaggccgac aaggatcagc gaaccctgga tggcggggcc
     2881 gagaccgcca cccggttcgc cattaggcga gggaatcttc gtaaacaggt ccaggttgat
     2941 atagggtagc cctgcccgta ggacatacaa cagcaagaac agcaatacgc acacggcaaa
     3001 cgctgttgcc agcaacgaga aggtgataat gatgatatta ctgattctgc gccgggcatt
     3061 gtgtgcagtg cgcggcggct ggaacggatt ctccaccgac tgcttctctg aaacggtcgt
     3121 tgtgttcata cgacaagtcc tcccacccgg cccatt
//
LOCUS       DASZAK010000718         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_231077, whole genome shotgun sequence.
ACCESSION   DASZAK010000718 DASZAK010000000
VERSION     DASZAK010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231077"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1004
                     /locus_tag="VGT44_12660"
     CDS             126..1004
                     /locus_tag="VGT44_12660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2581697.1"
                     /translation="MQYGVYVPNFGPYGDARVLADLAFEAEEAGWDGFFLWDQVSKTT
                     LTPGVDAMVSPWVALAAIALRTRTIRLGPLVTPLPRRRPWMVARETVSLDHLSAGRLV
                     LGVGSGGGYYDFEALGEASDPKTLAALLDEGLEVLTGLWSGDLYQHTGPAYQIKEAQF
                     LPCPLQSPRIPIWVAGMWPAKAPLRRAAGFDGVIPIGRDLPLTAMLAPAQMQAVASYI
                     ASQPMYTTPFEVVHMGITAGTDVAYDREVVANYQRVGVTWWVEKILPDLWGSWTNWPL
                     EEMRARIRQGPPIAAW"
     gene            complement(1061..1771)
                     /locus_tag="VGT44_12665"
     CDS             complement(1061..1771)
                     /locus_tag="VGT44_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEV2581698.1"
                     /translation="MSVFSAESLQLRRELAYACRILAANGHNDTIYGHVTYREAGTQT
                     FWMKPAALGLDEITPETLIRVDLEGRVVEGELPRHLEWPIHAEILRARPDTTCVVHTH
                     PIYSIAFATTGEPLHALSHEGAQFTPPDVPRFTQTSDLITTRELGEEVARTMNGHLAC
                     FLVNHGIVVAGNTIEQAVVSAINLERASQVQLLAAASGQPFRWTSDEEIAGKREKIFG
                     AGMLRNVWNYYCRRIGPL"
BASE COUNT          451 a          602 c          670 g          459 t
ORIGIN      
        1 ggtagcgcga cagggaaggc ggcgagttcg gcgaactgaa gcacggacat gatacaatac
       61 aaaaactgtg ttccgcgccc gcatgtgtag agcaggcaat cactgtcgca gtcgaggaga
      121 gacctatgca gtatggcgtc tacgttccca attttggacc ttatggcgat gccagggtac
      181 ttgctgacct ggcgtttgag gcggaagaag ccggttggga cggctttttt ctctgggacc
      241 aggtctcgaa gacgaccctg acgccgggcg tcgatgccat ggtgagtccg tgggtggcgc
      301 tggccgcgat cgcgctgcgc acacgcacga tccgcctggg cccgctcgtc acacccttgc
      361 cgcgtcgccg cccgtggatg gtggcgcggg agaccgtctc gctagatcac ctatccgcgg
      421 gtcgattggt cttaggagtg ggatcggggg gtggctatta cgatttcgaa gcgcttggcg
      481 aagcctccga ccccaaaacg ctggcggcgc tgctggacga gggtctggag gtgctcaccg
      541 ggctatggag tggcgatctg tatcagcata cagggcctgc atatcagatc aaggaagcgc
      601 agttcctgcc gtgtccgctc cagtctccac gcatccccat ctgggtggct ggcatgtggc
      661 cggcgaaagc gcctctgcga cgcgcggcag gcttcgatgg cgtcatccct atcgggcgcg
      721 atctgcccct gaccgccatg ctcgcgccgg cgcagatgca ggccgtcgcc agctacatcg
      781 cctcccaacc catgtacaca acgccattcg aggttgtcca catgggtatc accgcgggca
      841 cggatgtcgc atacgatcgc gaggtcgttg caaactacca gcgggtgggc gttacctggt
      901 gggtggaaaa gatcctgccc gatctttggg ggagttggac taactggccc cttgaggaga
      961 tgcgcgcgcg gatacggcag ggaccgccga ttgccgcctg gtaattccca ctcatcgtca
     1021 ggacgttctt ttctccagga acagaagcat gctcctacgc ttacagcggc ccaatacgac
     1081 ggcagtaata gttccacacg tttctgagca tgcctgcgcc aaagattttt tcgcgttttc
     1141 ctgctatctc ctcatcagag gtccagcgaa agggctgacc ggaggcagcg gccagtagtt
     1201 gcacctggct agctcgctct agattgatag ctgagacgac ggcctgctcg atcgtgttgc
     1261 cagcgacgac aatgccatgg ttgaccagaa aacacgcaag atgtccattc attgtacgag
     1321 caacttcctc tcccagttcg cgagtggtga tgagatcgga ggtctgggtg aagcgcggga
     1381 catcgggcgg ggtaaactgc gctccttcgt gcgagagggc atgcaatggc tctcctgtag
     1441 tggcaaaggc aatggaatag atggggtggg tatgcacgac acaggtggtg tcgggcctgg
     1501 ctcttaaaat ctcagcatgg attggccact ccaggtggcg gggcagttca ccttcgacaa
     1561 ctctcccctc cagatcaaca cgaatcagcg tctctggcgt gatctcatcc aggcccagcg
     1621 ccgccggttt catccaaaag gtctgagtgc ctgcctctcg gtacgtgacg tggccgtaga
     1681 tggtatcgtt gtggccgttg gctgccagga tgcgacaggc gtacgccaac tcacgacgca
     1741 gctgcagact ctctgctgag aagaccgaca tgatgctctc cttgccaatg ctcattcagt
     1801 gtattctgcc acaatgctcg gtttatccgc aacgcacgtg aaaagcagga aggaatgttc
     1861 tctcagctat caccgataga acagcctacc aggacttgga aaggttggtc gaacggggca
     1921 ggttgaaagg cagtagacag agggccgcat actggtatgt gcgggtatag cttcacattt
     1981 ggagccacaa gagcaaaaaa cgggcgctgc tacctgtagc aacgcccgtg cttttcgcag
     2041 aaaagcgacg aatttcaaca aacaggatgc tgtattttga gcgtgagatg aacgttagag
     2101 gaccctcgag gcgagttcat gccggtggag ggatgcggca tggcttgtct atagaccttc
     2161 gttatggttc tcaatacgct ca
//
LOCUS       DASZAK010000719         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_267792, whole genome shotgun sequence.
ACCESSION   DASZAK010000719 DASZAK010000000
VERSION     DASZAK010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267792"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VGT44_12670"
     CDS             complement(<1..431)
                     /locus_tag="VGT44_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEV2581699.1"
                     /translation="MTKLYIVLEQAMAIVSQNGQGWKGEIRLPGNNTQCVAVDPLRPE
                     YVYCGTFGQGLWRSRNAGLSWEQVGEGITYNQITSVAVSETERIGDIGVVYAGTEPSA
                     LFRSEDGGNSWRELAALHQLPSAPTWSFPPRPYTSHIRWITP"
     gene            674..2545
                     /locus_tag="VGT44_12675"
     CDS             674..2545
                     /locus_tag="VGT44_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HEV2581700.1"
                     /translation="MQENTSTLHPRPQLTRARWIDLGGEWGFTYDDEACGINEHWQEK
                     PEEFTRTIKVPYPPESKASGIGDNGFHAIVWYRRTFEVDDQDAGKRLLLHCGAVDYSA
                     HVWVNGHLVATHEGGQTPFSADITSALHPSREQVVVIRAEDEPKDLTQPRGKQDWQEP
                     PHEIWYHRTTGIWQPVWLEPISSTYISQIRWTPDLDRGLLSFAITLEREEDAPLNLRV
                     KFNLHGVDLADDTYMMRGTELKRQIAFDPAAMVDGGDRLMWSPRNPNLIDVTLTLLEG
                     KKIIDEVQSYIGIRSVSATNGRFLLNGLPYYLRLVLEQGYWPETHLAAPDDDALRREV
                     ELIKEMGFNGVRIHQKVEDPRFLYWCDRLGLLVWGEMANAYVFSPQAVGRLTREWIEA
                     VNRDYNHPCIVAWVPFNESWGVPNVAHDEAQQHYVQALYHLSKTLDPTRPTVGNDGWD
                     NFVSDIFGVHDYSFDGSVLRDRYGSFEAVERTLREVQPNYHSLILPGYRREGEPIMLT
                     EFGGISFHPDGGGPGYGYSTVNDDESFLARYGELLDAILNSPAIAGFCYTQLTDVEQE
                     TNGLLTENREPKLNPADVRAITSQVSAAVPGDVITKMLQAHSVTSFTSAALKSKPGS"
     gene            complement(2559..>2772)
                     /locus_tag="VGT44_12680"
     CDS             complement(2559..>2772)
                     /locus_tag="VGT44_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581701.1"
                     /translation="DNTGSVVATQSLARALDIGIFYQNAEQLIAQLHDQERMARLRRN
                     IRRERERFTFDYHADTLIDFFRRMIG"
BASE COUNT          613 a          757 c          788 g          614 t
ORIGIN      
        1 ggggtaatcc agcggatatg gctggtatag ggccggggag gaaaactcca ggtgggcgcg
       61 gaaggcaatt gatgcagcgc tgccagctcg cgccaggagt tgccgccatc ttcagagcgg
      121 aagagcgcgc ttggctccgt cccggcatag actacgccga tatctcctat gcgttcagtc
      181 tcgctgactg ccaccgatgt aatttgattg tatgtaattc cttcgccgac ctgctcccag
      241 gaaagaccgg cattccggct gcgccacaag ccttgtccaa aggtgccgca gtagacgtac
      301 tctggtcgca gcgggtctac cgcaacgcat tgggtgttgt tacccggcag gcggatttcc
      361 cctttccagc cctggccatt ctggctaacg attgccatcg cctgctccag gacaatgtag
      421 agctttgtca ttgtctgctc ctttctacga aaagcggatc tgtaccctcg ctcgtatgga
      481 cataaaagaa cctctctgga gagtgtagtg tgcgagaaga tgccctgcat cgtccgaaag
      541 gtcagtcatg cacctgtacc ttgagctatt cagggatatg acctgctcga caggttcgcc
      601 atgctcaggt ataatgggaa cactaaatcc acactttttc ggttgtgttt gctttgtaga
      661 aatgaagaag ggtatgcaag agaatacaag tacgctccat cctcgtcctc aactcacgcg
      721 cgctcgatgg atcgacctgg gcggcgagtg ggggttcacc tatgacgatg aagcttgtgg
      781 catcaatgaa cactggcagg aaaagccaga ggaatttacc cgaaccatca aggttcccta
      841 cccgccggag tcgaaagcaa gtggtatcgg cgataatggc tttcacgcca tcgtgtggta
      901 ccgccgcaca tttgaagtag acgatcaaga tgccgggaag cgtttgcttt tacattgtgg
      961 cgcagttgat tactcggcac atgtctgggt gaatggccat ctcgtcgcca cgcatgaagg
     1021 aggacaaact cccttttctg ccgacatcac gtctgcgctg catccctcca gggaacaggt
     1081 cgtggtcatt cgcgccgaag acgagccgaa agacctgacg cagccgcgcg gcaagcagga
     1141 ttggcaggaa ccgccccatg aaatctggta ccatcgcaca accggcatct ggcaacccgt
     1201 ctggcttgag ccgatttcct cgacgtacat ctcgcagatt cgctggacgc ctgatctaga
     1261 tcggggcttg ctctctttcg cgattacgct cgaacgcgag gaagacgcgc ccttgaacct
     1321 gcgcgtgaag ttcaacctgc acggggtaga cctggccgat gatacctata tgatgcgtgg
     1381 taccgagttg aaacggcaga ttgcctttga cccggcagct atggtcgatg gcggtgatcg
     1441 acttatgtgg tcgcctcgca acccgaatct catcgatgta accctgacat tgcttgaagg
     1501 taaaaagatc atcgatgaag tgcagagtta catcggcata cgcagcgtca gcgcgaccaa
     1561 cgggcgcttc ctgctgaacg gcctgccata ttatctgcga cttgtgctgg agcagggcta
     1621 ctggccggaa acccacctgg cagcaccaga cgacgatgcg cttcgccgtg aggtggaatt
     1681 gatcaaagaa atgggattca atggcgtgcg aattcaccag aaggtcgagg acccgcgctt
     1741 cctctactgg tgcgaccgtc tgggcctgct ggtatggggt gagatggcga atgcctacgt
     1801 attttcgccg caggcggttg gtcggctaac gcgcgaatgg attgaggccg tcaaccgcga
     1861 ctacaaccat ccctgcatcg tggcctgggt gcctttcaat gaaagctggg gtgtgccaaa
     1921 tgtggcgcac gacgaggcgc agcagcacta tgtccaggcg ctttatcatc tgagcaaaac
     1981 cctcgatccg acacgtccga ccgtcggtaa cgatggttgg gacaatttcg tcagcgacat
     2041 cttcggcgtc cacgattatt ccttcgatgg ttctgtactg cgcgatcgct atggcagctt
     2101 cgaagcagtc gaacggacat tgcgcgaggt gcagccgaac taccactcat tgatcctgcc
     2161 tggctatcgt agagaaggcg agccgatcat gcttaccgag tttggcggta tcagctttca
     2221 cccggatggt ggcgggcctg gctacgggta tagcactgtg aacgatgacg agagctttct
     2281 tgccaggtac ggggaactgc ttgatgccat tttgaacagc ccggctatcg caggtttttg
     2341 ctatacacag ttgaccgatg tagagcagga gaccaatggg ctgctcactg aaaaccggga
     2401 gccaaagctg aatccggccg atgtgcgcgc catcaccagc caggtttcag cagccgtccc
     2461 aggtgatgtc atcaccaaaa tgctgcaggc acatagcgtc acctccttta ccagcgctgc
     2521 gttgaaatca aaaccaggct cataggtcgt catcgcacct acccaatcat acgcctgaaa
     2581 aaatcgatca aagtgtcggc gtggtaatcg aaggtgaagc gttcgcgctc acggcgtatg
     2641 ttgcgccgca gcctggccat gcgctcctgg tcgtgtagtt gcgcgattaa ttgctccgcg
     2701 ttctggtaga atatcccgat atcaagggcg cgggcgagtg actgcgtagc aacgaccgag
     2761 cctgtattgt cg
//
LOCUS       DASZAK010000720         5450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_623494, whole genome shotgun sequence.
ACCESSION   DASZAK010000720 DASZAK010000000
VERSION     DASZAK010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623494"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /locus_tag="VGT44_12685"
     CDS             complement(<1..837)
                     /locus_tag="VGT44_12685"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2581702.1"
                     /translation="MANLGYIGLGAMGGRVARRLLAAGHTVTGYNRTRPKAQWLLDAG
                     MRWGESPRVVAESADIVFTMVTNTQALHEVFNGPDGILAGLGPGKVYIDMSTVSPAAS
                     RHLAGQVAAKGAHMLDSPVSGSVITLEQGKLSLMVGGDRATFEQVKPILLDIGPKVTH
                     VGENGQAVLMKIAVNLNLQVQFLAFCEGLFLAIKGGVPLETALEVMLNSVIASPSLQY
                     RTQFIREMPDEAWFDVNMMQKDMLLALELGRELDVPLPTVAISNEFLTAARAMGLAKH
                     DFA"
     gene            complement(849..1550)
                     /locus_tag="VGT44_12690"
     CDS             complement(849..1550)
                     /locus_tag="VGT44_12690"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEV2581703.1"
                     /translation="MYTPVRSSKIFERIAEQIERRILSGELHSGDRLPTERELAEQFG
                     ASRTAVREAMKSLAQRGLVDMKPGRGTRVIDGTSKAMQHSLGLMLKVGQAGLSDNLVE
                     VRGFLEPEIAALAAQRASEEHIAAMQEAVDIMDASLGNVDAFIDADNSFHQALAKGTQ
                     NVLILALVDSIVNLLSEQRKQIFSVPGGPERGQMHHKQILDAVRLGDAQAAREAMRAH
                     LEQVRTDVTSAKQPK"
     gene            1846..3171
                     /locus_tag="VGT44_12695"
     CDS             1846..3171
                     /locus_tag="VGT44_12695"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2581704.1"
                     /translation="MLQRYFGNNKTILSVVTSFFMILAGLSACAGQATTPTFTNPNPI
                     VIGASLSLTGNKNFDFSGDGPAMRQGYQLWADMVNNNGGLLGRPVKLIILDDNSDPNL
                     VVKNYETLIKQDHVDLLFGPFSTLLTKAALDVPGISKYAFIEGAGGAYSVFQKAQQKG
                     LNNLFDVSLPITNNLTTFVYYLLSLPAADRPTTAAYLTSDDPFTVPQVQLAETLLEQA
                     GIKTVYTNAENSYINVNKNVPYPEGGNVKVATADAQQMIKQNPQVVILGSLLPDIQIV
                     VPLFKQYHFNPKALIATAGPDLGQQFLDAIKGVKYAEGVFVPNGWYPQANTYQNAEMV
                     SAYTGQYKVQAGQINGDVAEAFSVGQVLQQAASKINSIDNAALIKELQSSGDVFNTVQ
                     GTAQFDVRDGGQNTQAISYLFQWQGTQFIPVYPYSVAAENPTYPKPATY"
     gene            3384..>5450
                     /locus_tag="VGT44_12700"
     CDS             3384..>5450
                     /locus_tag="VGT44_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV2581705.1"
                     /translation="MFRYLSNVPIFRRLFIAFAVSTIIPGIIIILLGNFYVNSSNTRG
                     QAVRTSFDAQNTASLEETNLQRMNALLNAQFYQVFASQSHVINDPSLYASGGLDSGEI
                     KELEADFDRTLPIYISQYGLNSSNMSTVRSILLTDDPAAGNAIINAQQAALQNVATFD
                     WNGKINGKQTEYKYYQDQLLGALQGLQQQLLSSSTPLTPAQVNADYNTDYPLLFNANQ
                     QFTNLKNDWEQVVSEAVTMGKAVTAVGPSQIQPIFISTALALILIIMVVTLTGWIVNV
                     TITQPLRRLASLTGRIAKGNTQARSTVVGHDEIAVVSNAMNKMLDNIVHLIQEVQSQR
                     DALQAQVEKLVSEVSGVGEGDLRIQAEVTADALGVLADSFNYMVEELSSLIVRVKMVS
                     QEVENSTTMTFERMTHLVENADIQIQQIGEAAVEVEQVADYSRQVAERAQSLHVIARE
                     ARHTAEVGRESVQQTVQGMGRIHTYVQDTASKVQALGDSSRQIDDIVGAIANIAHQTN
                     RLALDAAIQAAMAGENGKGFGAVAADIRRLAERAKDQASLIARIVRSVREDIGAAAVS
                     MQDTERETSSGAKLAEEAGSSLFSIFSVVDRQGQEIEVINQVAARQLQSSNTVVQIMQ
                     GVSEATQQSSSSTREAAQNMERLARLAEQLLSSVEAFKLRDNVDYYPPSNVTVMPEDD
                     ADNYMTV"
BASE COUNT         1260 a         1574 c         1484 g         1132 t
ORIGIN      
        1 ggcaaagtca tgcttcgcca atcccatagc gcgtgcagcc gtgagaaact cgttgctaat
       61 tgccacggtt ggtagcggta catcaagttc tcgccctagc tctagcgcca gtagcatatc
      121 tttctgcatc atattcacgt caaaccaggc ttcgtcaggc atttcgcgaa tgaattgggt
      181 acgatattgc agcgaagggg aggcaatcac gctgttcagc atgacctcca gcgccgtttc
      241 gagcggaaca ccgcccttga tggccagaaa tagcccttca cagaatgcca ggaattgcac
      301 ctgcaaattg aggttgacgg caattttcat caagacggcc tggccgttct cgccgacatg
      361 cgtcaccttt ggtccaatat cgagcaagat aggcttcacc tgctcaaacg tggcgcggtc
      421 gccgccgacc atcagcgaga gcttgccctg ctcaagcgtg ataacgctgc ccgatacggg
      481 cgaatccagc atgtgcgcgc ccttggcagc cacctgtccg gcgagatgac gcgaggcagc
      541 cggactgacg gtactcatgt cgatgtagac tttgcccggc ccaagccctg ccaggatgcc
      601 gtcggggcca ttgaacacct cgtgcagcgc ttgagtattt gtgaccatcg tgaaaacaat
      661 atcagcgctc tcggccacga cgcgcggcga ctcgccccaa cgcatcccgg catccagcag
      721 ccactgcgcc ttgggcctcg tgcggttgta gcccgtgacg gtgtggcccg ccgccaggag
      781 ccgacgggcg acgcggccgc ccatcgcgcc caggccaatg tatcccaggt ttgccatctc
      841 gacctccttt attttggttg ttttgccgac gtgacatccg tgcgtacctg ttccaggtgc
      901 gcgcgcatcg cctcgcgagc cgcctgagca tcgcccaacc gcacggcatc gagaatctgt
      961 ttgtgatgca tttgcccgcg ttcagggccg ccgggaaccg aaaatatttg cttgcgctgc
     1021 tcgctcagca ggttcacgat agaatcgacc agcgccagga tcaggacatt ctgggtgcct
     1081 ttggcgagcg cctggtgaaa gctgttgtcc gcatcgatga atgcatcaac gttgcctaag
     1141 ctggcgtcca tgatgtccac tgcctcctgc atggccgcaa tgtgttcttc gctggcgcgc
     1201 tgggcagcca gggccgcaat ttctggttca agaaagccgc gcacctccac caggttgtcc
     1261 gataacccgg cctggccgac tttcaacatc agccccagcg aatgctgcat agcttttgag
     1321 gtgccatcga tcacccgcgt ccctcgcccc ggcttcatat ccaccagccc gcgctgcgcc
     1381 agcgacttca tcgcctcgcg aacagccgtg cggctcgctc caaactgctc agccagctcg
     1441 cgttcggtcg gcaggcgatc gccactgtgt aattccccgc tcagaatgcg ccgttcgatc
     1501 tgctcggcga tgcgttcaaa tattttgctg gagcgtaccg gagtatacat gctatccaat
     1561 gggatatgcc tccgtgttcc caaaagttga tatgattatc ataccaactt cctacacacg
     1621 aaaactatac ctgccctatt tactcctgtc aagtctttca ggtaggaaat tcaggtgagt
     1681 ctcgccccaa aacgtttcat tttagcttca agtaggatac agtaaagtgg caacgattta
     1741 aggggtgtat tcagttacgg catggcgacc gcaagatatt taaacatcga tacccataca
     1801 tgctcatttc gcgtcggagc ttgatcagtc aaaaggagga gagacgtgtt acagcggtat
     1861 ttcggcaaca ataaaaccat cctctcggtg gtgacgagtt tctttatgat cctggccggg
     1921 ttgagcgcgt gcgcggggca ggctaccaca cccactttca ccaacccgaa cccgattgtg
     1981 attggggcct cgctttccct gacagggaac aagaactttg acttctccgg tgatggacct
     2041 gctatgaggc agggctacca gctgtgggcg gatatggtca acaacaacgg cggtttgctt
     2101 ggtcgcccgg tcaagctgat catcctggac gataacagcg accccaacct ggtcgtgaag
     2161 aactacgaga ccctcataaa acaagatcat gtagacctgc tctttgggcc attctctacc
     2221 ctgctgacaa aggccgcgtt agatgtacct ggtatctcga agtatgcctt tatcgagggc
     2281 gcaggtggcg cttactccgt cttccagaag gctcaacaga agggactgaa caacctcttc
     2341 gacgtgtcgc tgccgatcac caataacctg actaccttcg tctactatct cctttcgctc
     2401 ccggctgcgg atcggcccac caccgccgcg tatttgacct cggacgaccc attcaccgtc
     2461 ccgcaagttc aactggcgga aacgctgctg gagcaggctg gaatcaagac agtctataca
     2521 aatgcggaga acagctatat caatgtcaat aagaacgttc cgtaccctga aggtggcaac
     2581 gtcaaagtag cgactgctga tgcacagcag atgatcaagc aaaacccaca ggtggtcatt
     2641 cttggttcgc tgctcccgga tatccagata gtggtaccgc tgttcaagca atatcacttc
     2701 aatccgaaag ctctcattgc gaccgcaggc ccggacctgg ggcagcagtt tcttgatgcg
     2761 attaaaggcg tgaaatacgc tgaaggtgtg ttcgtgccga acggctggta cccgcaggcc
     2821 aatacatacc agaatgctga gatggtgagc gcatacaccg gccaatacaa ggtgcaggcc
     2881 ggccagatca acggcgatgt cgcggaggca ttttcggtcg ggcaggtgct gcaacaggcc
     2941 gccagtaaaa tcaacagcat cgacaacgcc gcgctgatta aagaattgca aagtagcggc
     3001 gatgtcttca acaccgtgca gggaacagca cagttcgacg ttcgcgacgg cggccagaat
     3061 acgcaagcta tctcctacct gttccagtgg caggggactc agtttattcc ggtctaccca
     3121 tactccgtag cagcggagaa tccgacctac ccgaaacccg ccacctatta gaaacaaaag
     3181 ggaagagata gcctgccggg gcgctaggag aatcaggtca gttgacttgc aataatagct
     3241 atttcttccc attgtgatgt acactgtttg ttgtcctgga aatatctaag gaaactggct
     3301 cagttagtgt tgatgccgcg cagcagcgtg gatgtaccgg agaggcgagt ggtgtttgct
     3361 gcaagagaag ggatgaattt gcgatgtttc gatatttaag taacgtgcct attttccggc
     3421 gcctcttcat tgcgttcgcc gtatcgacga tcatacccgg catcatcatt attttgctgg
     3481 gcaatttcta cgtcaactct tcgaatacac gcggtcaggc ggtcaggaca agttttgacg
     3541 cacagaacac tgcatccctg gaagagacaa acctccagcg catgaacgcg ctgttgaacg
     3601 cgcagtttta ccaggtattc gccagccaga gccatgtcat caacgatcca tcgctctatg
     3661 cctccggcgg gctggactca ggcgaaatca aagagctgga ggcggacttc gaccgcaccc
     3721 tcccgattta tattagccag tacgggctga attcctctaa tatgagtaca gtgcgcagca
     3781 tcctgctcac ggacgacccg gcggcaggca atgcaatcat caatgcccag caggcggcgc
     3841 tgcaaaatgt agcaacattt gactggaacg ggaaaataaa cggcaagcag acagagtaca
     3901 agtactacca ggatcaactg ttgggcgcgc tccagggctt gcagcagcaa ctactcagct
     3961 caagcacgcc tctgacgcct gctcaggtca acgcggatta caacaccgat tatcctctac
     4021 tgttcaatgc gaaccagcaa ttcaccaacc tgaagaacga ctgggagcag gtcgttagcg
     4081 aagcggtgac aatgggtaaa gcggtgacag cagtcggccc ctcgcagata caaccgatct
     4141 ttatctcgac cgctctggcc ctgatcttga ttatcatggt cgtgaccttg accggctgga
     4201 ttgtgaacgt cacgattaca cagccgctgc gtcgacttgc ctcgctgacc gggcgcatcg
     4261 ccaagggaaa cacgcaggcc cgctccacag tcgttggtca tgacgaaatc gcggttgtct
     4321 cgaatgctat gaacaaaatg ctcgacaaca ttgtccacct gattcaggaa gtacaatccc
     4381 agcgtgacgc gctgcaagcg caggtggaga agctggtgag cgaggtaagt ggcgtgggtg
     4441 aaggcgacct gcgcatccag gccgaagtaa cggcggacgc tctgggcgtg ctggccgact
     4501 cgttcaacta catggtggag gagctgagca gcctgatcgt gcgcgtgaaa atggtctcgc
     4561 aggaggtcga gaattcgacg acgatgacct ttgagcgcat gacccacctg gtcgaaaacg
     4621 cggatatcca gattcagcag atcggcgagg ccgccgtcga agtcgagcaa gtggctgatt
     4681 atagcagaca ggtggccgaa cgcgcccagt cgctccacgt tatcgcgcgt gaagcccgcc
     4741 acaccgccga ggtcgggcgc gaatcggtgc agcagacagt acagggcatg ggacgcatcc
     4801 acacctatgt gcaggacacc gcctcgaagg tgcaggcgct gggcgatagc tcgcgccaga
     4861 tcgatgatat cgtcggcgct atagccaaca ttgcgcacca gaccaaccgc ctcgccctgg
     4921 acgccgctat ccaggcggcc atggccggcg aaaacggcaa aggcttcggc gcggtggctg
     4981 ccgacatccg ccgcctggca gaacgcgcca aggaccaggc cagcctgatt gctcgcatcg
     5041 tccgcagcgt ccgcgaggat atcggcgcag cagcggtttc gatgcaagat accgagcgcg
     5101 agacatccag cggcgcgaag ctggctgaag aagcgggatc gtcgcttttc tcaatcttct
     5161 ccgtcgttga tcgacaagga caagagatcg aggtcatcaa ccaggtagcg gcgcggcagt
     5221 tgcaatcttc aaatacggtc gtgcagatca tgcagggcgt ttctgaagcg acacagcaaa
     5281 gcagctccag tacgcgcgaa gctgcccaga acatggaacg cctggcgcgc ctggcagaac
     5341 aactgctttc ctcggtcgag gcattcaagc tgcgcgataa cgtcgattac tatccaccct
     5401 ctaatgtaac ggtcatgccc gaagacgatg ccgataatta tatgacggtg
//
LOCUS       DASZAK010000721         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_445596, whole genome shotgun sequence.
ACCESSION   DASZAK010000721 DASZAK010000000
VERSION     DASZAK010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445596"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VGT44_12705"
     CDS             <1..933
                     /locus_tag="VGT44_12705"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV2581706.1"
                     /translation="HRLVKATAQFAQGDYSQRVAVTRQDEIGQLERQFNSMAEQLVES
                     IAQQQALTEQHARLEERARIEQELRTAQYIQRALLPKEVPTLPGWQLVPVYRPAREVG
                     GDFYNFLPAEDGRLGIVIGDATDKGVSAALLMATTCTMLRTASQRAASPGEVLARVND
                     LLATTIPAGVFVTCFYAMLDPGSGRLCYANAGHDLPYLRHEGEVSELYATGMPLGLMP
                     GTQYEEKEATLVDGDSLLLYSDGLVEAHNPQRDMFSFSYLMALLQEDIAGQELIDFLM
                     SKLALFTGPDWEQEDDVTLLTLQRFKVIDQERRD"
     gene            complement(956..1777)
                     /locus_tag="VGT44_12710"
     CDS             complement(956..1777)
                     /locus_tag="VGT44_12710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2581707.1"
                     /translation="MMDNYAPPGRNELVLHELEQQLRDVEQRCREMQREIQHLREMNQ
                     LKDQFLMMASHELRTPLTSVQGYLELMAEYQDELSPERCRAYLQKAQRSCEELVMLFG
                     NVMDVSRLDEEMHPAQMETVSVQKMIESVMYLIEPRVLKEQREIQLHIPPRLQVRANP
                     GRLRQALLNISINALKYSPPQSPIIFAARVDPDSNNAVDISITDMGRGIAPPDQARVF
                     QLYERLERDVQSAVQGSGLGLYISNLFIEAMGGKIWIESEGIAGQGATFHMQLPA"
     gene            complement(1929..2408)
                     /locus_tag="VGT44_12715"
     CDS             complement(1929..2408)
                     /locus_tag="VGT44_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581708.1"
                     /translation="MLRGVVRDAVRSPAMAVLVEIGCSVSQAESAQTAIEGLCKETGS
                     LLVGRRNNKWTRKVLYIVQFPHLESARKLMAALRQSRQHGVIESFRYMPGDLVHISFH
                     PPVNLQEQMRDFPLRPEESWFPAIHHPHQVYVSLSMPVMLAKQAAWLVAHEVEWSFV"
BASE COUNT          543 a          690 c          708 g          581 t
ORIGIN      
        1 catcgcctgg tgaaagctac cgcgcaattc gcccaggggg attattcaca gcgcgttgcg
       61 gtcacgcggc aggatgaaat agggcagctg gaacggcaat tcaattcgat ggcggaacaa
      121 ctggtcgaga gtatcgcgca gcagcaggcc ctcaccgagc agcacgcgcg cctggaagag
      181 cgcgcccgca tcgagcagga gctgcgcaca gcccagtaca ttcagcgcgc tttgctgccg
      241 aaggaggtgc cgaccctccc cggctggcag ctcgtgccgg tctaccgccc ggcgcgcgag
      301 gttggcggcg acttctacaa tttcctcccg gctgaagatg gccggttggg gattgtgatt
      361 ggcgatgcaa cggataaagg tgtctcggcg gccctgctga tggcgaccac ctgtaccatg
      421 ctccgtaccg cctctcaacg ggcggcttcc cctggcgagg tgctggcgcg ggtgaatgat
      481 ttgctcgcta ccaccattcc agccggggtg tttgtcacat gcttctatgc catgcttgat
      541 cctggcagcg ggcgtttatg ctacgcaaac gcgggccatg acctgcccta cctgagacac
      601 gagggagagg tttccgaact gtacgctacc ggtatgcccc tgggtttgat gccgggtacg
      661 cagtacgaag agaaggaagc caccctggtc gatggcgata gcctgctcct ctatagtgat
      721 ggcctggtgg aggctcacaa cccgcagcgt gatatgttta gcttctccta tttgatggcg
      781 ctccttcaag aagatatcgc ggggcaggaa ctgatcgatt ttctcatgag caagctggca
      841 ctgttcaccg gccccgattg ggagcaggag gatgatgtca cgctgttgac attacagcgc
      901 tttaaggtga tcgatcagga gcggcgcgat tgatctagat gtggaagggc gaccatcatg
      961 caggtaactg catatgaaac gtcgctcctt gccctgcaat accctcgctt tcgatccaga
     1021 tttttccgcc catcgcctcg atgaagaggt tcgagatgta taaacccaga ccggaacctt
     1081 gtacagcgct ctgtacatcg cgctcaagac gttcgtaaag ttggaagacg cgcgcctggt
     1141 ccggtggcgc gattccccgg cccatgtcgg tgatgctgat atcgacagcg ttgttggagt
     1201 cgggatcgac tcgtgcagca aatatgatgg gcgactgggg aggcgagtat ttaagggcgt
     1261 tgatgctaat attgagaagc gcctggcgca ggcggcctgg attggctcgc acctgtagcc
     1321 gaggcggtat gtggagttga atctcccgct gttctttcag aacacgcggc tcaatcagat
     1381 acatgacact ttctatcatc ttctggactg agacggtttc catctgtgcg ggatgcatct
     1441 cttcgtccag gcgactcaca tccatgacat tgccgaagag cattaccagt tcttcgcagc
     1501 tgcgttgcgc cttttggaga taagcccggc accgttcagg agaaagctca tcctgatatt
     1561 ctgccatcaa ttcgagatag ccctgcacag aggtgagtgg tgtgcgtagt tcgtgtgagg
     1621 ccatcatgag aaactggtct ttaagctggt tcatctctcg tagatgttga atctccctct
     1681 gcatttcccg gcaacgttgt tcaacatcgc gaagttgctg ttctaactca tgcaagacga
     1741 gttcatttcg gccaggcggc gcgtagttat ccatcatacc tacctcatag gctccaccct
     1801 tgccacttcc cgcgctggca gtaggtatta actgagccta cgtctcaaga gagttagtat
     1861 acccggatgc attcataatt gtcacttcat acatccagta tagcagagga tagtcaatct
     1921 gcaaagtttc acacgaacga ccactctact tcgtgcgcga ccagccatgc agcctgtttc
     1981 gctagcatga cgggcatact caggctgaca tagacctggt gtggatgatg gatggcgggg
     2041 aaccacgatt cttcgggacg taaggggaaa tctcgcatct gttcctggag gtttaccggg
     2101 ggatggaagg agatatgcac caggtcaccg ggcatatacc gaaatgactc gatcacgcca
     2161 tgttggcgcg attgtctcag cgctgccatg agtttgcgcg cgctctccaa atgcggaaac
     2221 tggacaatat acaggacttt gcgggtccac ttattgttgc gacgaccaac cagcaggctt
     2281 cccgtctctt tacaaagccc ttctatggcc gtctgcgctg actctgcctg agaaacggaa
     2341 caaccaattt caaccaggac ggccattgcc ggactcctaa ccgcgtcgcg aacaacaccg
     2401 cgcagcatta tgatactcct ttagacagcg ctacgacttg ggacggtatg ggcttccagc
     2461 aaatccttta aggttagcag atcatgctcg atctggcgtc tgaccacatt ggcaactacc
     2521 ga
//
LOCUS       DASZAK010000722         9508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_194236, whole genome shotgun sequence.
ACCESSION   DASZAK010000722 DASZAK010000000
VERSION     DASZAK010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9508
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194236"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VGT44_12720"
     CDS             <1..318
                     /locus_tag="VGT44_12720"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2581709.1"
                     /translation="AQHHLQGTRITLSGSMLCQPFERTAASARLVQLAREAGRDLGIN
                     IQDAGSGGASDANTTAAMGIPTLDGLGAGGGLAHNPGEYIELEYLPKRIALITGLIRR
                     IGN"
     gene            319..1524
                     /locus_tag="VGT44_12725"
     CDS             319..1524
                     /locus_tag="VGT44_12725"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV2581710.1"
                     /translation="MNLGKPSIHNLPVNDITSLGIVARQMQQEAEARGESLPPPARLH
                     VGEPSFRTPEHIRQAAIESIQHEALTYGPPAGWPWLRELLAEKIARINGYAVTPQHVA
                     IGMGGTGALQAALLATVSAGDEVLVPDPHWPHYTIQLASCGATRVPYPLDPQAGWLPD
                     IAQLERLVTSRTRLLIINSPGNPTGAVFPPQLVADLLDFARRHDLVLLSDECYDEIVF
                     EGRHTSPASLLSFDEFNEGRVMCVYSFSKSYAMTGWRIGYLTCGAELVKSITSVLDGC
                     NTNISTAIQRAAAAALSGSQACVGEMRDAYRQRRDRAVQLLQEHGRYRYTPHGAFYLL
                     IDITSPDQPQRSSRQFALDLLRERTITAASGTSFGSVADHYVRISLAASEEEIERGVR
                     EICLFAQNG"
     gene            1677..2321
                     /locus_tag="VGT44_12730"
     CDS             1677..2321
                     /locus_tag="VGT44_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2581711.1"
                     /translation="MTTPRTEDEWLSLREAADMLGMHPATVRLWADRNELPSRRTNGG
                     HRRFRRADIEARLHHETERKPRPSAQLLVQSVLGRVRFAFTDGTLSAFPWYTHFNDAA
                     RDAYRSLGRRVLELLLRALTDATSDGDLRAEALQIGKAYGSITRESHVPVADAVRAFL
                     YFRSLVDESVLQLTQVQGGRDRAHLDIPWAESLYQIQTLTNEILPALIEAALSE"
     gene            complement(2348..2713)
                     /locus_tag="VGT44_12735"
     CDS             complement(2348..2713)
                     /locus_tag="VGT44_12735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2581712.1"
                     /translation="MIRLVLVDDQPSVRQGLRMRLTVEPDMTVVGEANNGREAMILVQ
                     QLAPDIVLMDVEMPVMDGIEVSAVMHASNPQTAVVMLSIYDDVSTRARARAAGAAAFV
                     HKSGAIEVLLATIRQVAEQ"
     gene            3051..3701
                     /locus_tag="VGT44_12740"
     CDS             3051..3701
                     /locus_tag="VGT44_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2581713.1"
                     /translation="MTIRIVIADDHHVVRRGLRTFLELDAEMVVVGEAGDGAEAVRLA
                     HQLQPDIVLMDLLMPVMDGIAATATIRRELPDTEVLALTSVLEDASVVGAVRAGAIGY
                     LLKDTEADALCRAIKAAVEGQVQLTPQAAARLMQVVSAPESPQPLTERETEVLRLLAQ
                     GQANKHIARSLLITEKTVKAHVSNILGKLGVQSRTQATLYAIRIGLVSANAPGTEW"
     gene            3718..6408
                     /locus_tag="VGT44_12745"
     CDS             3718..6408
                     /locus_tag="VGT44_12745"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2581714.1"
                     /translation="MNHSPASTPLGETGLPRSETDMRLSGSWLVLAQVVCLTLCVLSV
                     GLFVGSILSYIVNNYLFCTSTAAACFAHGQLTPGDVQRLQELGLSRGFIALYPIVLDS
                     LVSLGYLVVAAFLFWRKSDDRVALMAALSLALFPMVFNYGLTSALPSPWWFLANVLSF
                     VGSLGFILFYYVFPSGHFVPGFTRWLLVMALVYWGLNEFFPFPSFNPFYRFHLLNGLT
                     YLGLLSSAVVAQLYRYRRVSSPTERLQTKWVVYGEVMGQGVYVVLFTISLFVPSILST
                     GSLVSLIRLTVVYVLSLLTPLSIGLAILRSRLWEIDAIINRTLVYGILTVCVVGVYVL
                     VVGSLGALIGASGNLLIELLATGLVAVLFQPLRAWVQRGVNRLLFGQRDEPYSVITQL
                     SQRLEAQLSPDAVLSTIVETVAQALKLPYVAILLKQEETFVLAASAGELVGDSLDLPL
                     VYQAETIGHMRLAPRTPGEPFTPADRRLLDELARQAGLAAHAIQLTADLQRSHEQLEQ
                     RVAERTRELSSLLDISHTVASTLQLKPLLGLILDQLKLVIDYTGSSILIIEGEDFVFL
                     DHRGPVPQDQLVRLRFPLEGMGSIWQTIASGESILIEDVHEQTGLAQALRAAMGDLLT
                     TTFQYVCSWMAVPLILRDQVIGMLVLTSSEEQAFTQRQATLALAIANQAAIAIENARL
                     YEQAQELAALEERQKLARELHDSVSQALYGIALGAHTARTLLNRDPALVAQPLEYILS
                     LARAGLVEMRALIFELRPESLETEGLVSALLKQAAALQARHEVPVETDLCEEPSLPLK
                     VKQELYRVTQEALHNTMKHAEASQVKVRLGQTGETIILEIRDNGVGFDPAGFFPGHLG
                     LLSMQERIKNLGGVLSIESIPGQGTTIHARVPAREVIRRE"
     gene            complement(6416..6679)
                     /locus_tag="VGT44_12750"
     CDS             complement(6416..6679)
                     /locus_tag="VGT44_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581715.1"
                     /translation="MTAPSPEPEKQPRDTAYWAEEVSVLKVERVPTSALNLNVEGHQV
                     LSPLQGFGQLWQNAAVRTTLYTAITPLRRIRWRGRGRKERDQA"
     gene            complement(6755..9118)
                     /locus_tag="VGT44_12755"
     CDS             complement(6755..9118)
                     /locus_tag="VGT44_12755"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEV2581716.1"
                     /translation="MADTPLHRRWLGLARVGWVVVVVLALFLFVESILSVNALLHQIC
                     TGAAATCITHGLLAAGDVRRLHELGLSRDFYATYTIGIMSLSALGYWVVATFLFWRTS
                     DDRVALLAAFSLALFPMVINDGLINRLPSPWWSLAQVLSVLGFFCLVLFGCVFPSGHF
                     VPRFTRWLLVVAPVYWGFDAFFPSSSLNPLAPSQVLGGLIFLGIIGGIVVAQLYRYQW
                     VSSPEERQQTKWVVYGVSMGVGGHLVLFTLALFIPSLFQTGSLGDLIKLAASYGFVLL
                     VPLSLGFATVSSRLWEIDVLINRTLIYGSLTGTVALVYVGLVLSLQTLLRGIISQDNS
                     VVIVLSTLAIAALFQPLRHRLQRVIDRRFYRRTYDAAQTLTAFGTRLQQRDEVDLATL
                     SADLLAVVQITMQPTRGSLWLRPPAPGIEPSPTQVATPGDSSPVILVPTDPLVGLLLS
                     AHGTVETVRLHLDSPALRALQADGVKLAVPLVSQGELVGLLQLGPRKSEQDYSSDDRG
                     LLNQLAIQAAPAIQVAQLVREQQAQALERERLEQELCIAQQIQRAFLPRDLPELVGWQ
                     VATYYQPARAVGGDFYDFLPFADGRLGIVIGDVSGKGIPAALLMTTTRTILRSVARHE
                     ASPGQVLEQTNALLCPEMLPGMFVTCLYALLDPASGCLRYANAGHDQPYQRQHDRVSE
                     LWATGMPLGLMAGMTYEEKETMIADGDSVLWYSDGLVEAHNRERAMFGFPQLTKLLEE
                     QRDAPSLIDVLVQQLTVFTGPNWEQEDDVTLVTVQRSSGFGGSVGAK"
BASE COUNT         1898 a         2909 c         2832 g         1869 t
ORIGIN      
        1 gcccagcatc acctgcaagg cacgcgcatc acgctgagcg gcagcatgct gtgccagccg
       61 ttcgagcgca cagcggcgag cgcgcgcctg gtacagctcg ccagagaagc aggacgcgac
      121 ctgggcatca acatccagga cgcgggcagt ggcggagcat ccgatgccaa cactaccgcc
      181 gcgatgggca taccgaccct ggacggcctc ggcgcgggcg gcggcctggc gcataatccg
      241 ggagagtata tcgaactgga gtacctgccg aagcggatcg cgttgattac gggattgatc
      301 cggcgcattg ggaattaaat gaatctcggt aaaccatcca tacacaatct gccggtgaat
      361 gacattacga gcctgggcat cgtcgcgcgg cagatgcagc aggaagcgga ggcgcgcggc
      421 gaaagcctgc cgccgcctgc gcgcctgcac gtgggcgagc caagctttcg cacacctgag
      481 catatccgcc aggcggctat tgagtcgatt cagcacgagg cgctgacata tggcccgccg
      541 gctggctggc cctggctgag agagctgctg gccgagaaga tcgcgcggat caacggctat
      601 gctgtcacgc cacagcatgt cgctatcggc atgggcggca cgggcgcgct ccaggccgcg
      661 ctgctggcga cagtaagcgc gggcgatgaa gtattagttc ccgacccaca ctggccgcac
      721 tacacgatac agcttgcctc ctgtggcgcg acacgagtac catatccact cgatccgcag
      781 gcaggctggc tgcccgacat cgcccagctt gagcggctgg tcacgtctcg cacacgcctg
      841 ctcatcatca acagccctgg taatccgacc ggcgcggtct tcccaccgca actggtcgcc
      901 gatctgcttg actttgcccg gcggcatgac ctcgtactgc tctccgacga atgctacgac
      961 gaaattgtct tcgagggacg gcataccagc ccggcctcgc tgctcagctt cgatgaattc
     1021 aatgaggggc gtgtgatgtg cgtctactct ttctcgaaaa gttacgctat gaccggttgg
     1081 cgcattggct atctcacgtg cggggcagaa ctggtgaaaa gcatcacctc tgtcctcgac
     1141 ggctgcaaca ccaatatcag caccgccatc cagcgcgcgg cggcggcagc cctaagcggg
     1201 tcgcaggcat gtgttggcga gatgcgcgac gcctatcgcc aacggcgcga ccgggcagtg
     1261 cagctcctgc aggaacatgg ccgctatcgc tatacgccac atggcgcgtt ctacctgctc
     1321 atcgacatca ccagccccga ccagccccag cgttcatcac gccagttcgc ccttgatctg
     1381 ctgcgcgaac gcaccatcac cgccgcgtca ggcaccagct ttggctcggt ggcggatcac
     1441 tatgtgcgca tctcgctggc cgcgtcggaa gaggagatcg agcgcggggt gagggagatc
     1501 tgtttgttcg cacagaacgg ataaggactt ttccacaatt tagaacactt gttcacatac
     1561 ttatccacat tactcgccac aattgtgccg gttataatta gcgctacttg acgctatacc
     1621 aatcatccca tagaatgcgt gcaggaagca gaacgcgaag agtgccagga ggggatatga
     1681 caacgccacg gaccgaggat gagtggctga gcctgcgcga ggcagccgat atgctgggga
     1741 tgcatccggc aacggtacgg ctgtgggcgg atcgcaacga acttccatcg cgccgcacaa
     1801 atggggggca tcgccgcttt cgccgagccg atatcgaggc gcgcctgcac cacgaaacgg
     1861 aacgcaagcc gcgcccttcg gcgcagctgc tggtgcagag cgtgcttggg cgcgtgcgct
     1921 tcgccttcac cgatggcaca ctcagcgcat tcccctggta tacgcatttc aacgatgcgg
     1981 cgcgcgacgc ttaccgcagc ctgggacggc gcgtgttgga gctgctgctg cgcgcgctga
     2041 ctgacgcaac ttccgatgga gacttgcggg ccgaagcgct gcaaatcggg aaagcctacg
     2101 gctcaattac tcgcgaatcg catgttcccg tcgcggacgc cgtgcgcgcc ttcctctatt
     2161 ttcgcagcct ggtcgacgaa agtgtactgc aactgaccca ggtgcagggc gggcgcgacc
     2221 gcgctcacct ggatatcccc tgggccgaaa gcctatacca gattcaaacg ctcacgaacg
     2281 agattctccc tgcgctgatc gaggcggcgc tgagtgaata gtagtattcg ttcaggatgc
     2341 gatatctcta ttgttccgcc acctggcgga tcgtcgctaa cagcacctcg attgctccac
     2401 tcttgtggac aaacgccgca gcccctgctg cacgcgctcg tgctcgcgtg gaaacatcgt
     2461 catagatact cagcatgacg acagcggtct gtggattgct ggcatgcatc accgctgaga
     2521 cttcgatgcc atccatcacc ggcatctcga catccatcaa cacaatgtcg ggagcgagct
     2581 gttgcaccag gatcatggcc tctctgccat tgttcgcctc tccgaccacc gtcatatccg
     2641 gctccacagt gagtcgcatg cgtaacccct gccggacgct cggctggtcg tccacgagca
     2701 ctagtcgaat catgccaact tcatctctct catctcgcct tgcgtaccca ggccagagac
     2761 gccgacagat atgatataat gagaagcaca gcaactggtt gctctcatgc ggggtaccgc
     2821 gcattcattc attcaagcat ctcgtaggta ctcgctggag ggtctttccc caggtttacg
     2881 tcaagtatag gccgtgatga ggccccgcgt atcaggcatc aggccgattt ttcgctaggt
     2941 ctttaggcgt agaggcacgc tccgtcccgc catccaacca gacaccaatc gatgcgttgg
     3001 tggtgaacac cttggaatca gacgggatga gcagagaggg aggcgaggcc atgacgattc
     3061 gcattgtaat cgccgatgat caccacgtag tgcgtcgcgg actgcgcacg ttcctggaac
     3121 tggacgctga gatggtcgtc gtgggcgagg ccggtgatgg agccgaggcg gtgcgcctgg
     3181 cacaccagct gcagccagac atcgtcttga tggacctgtt gatgccggtg atggatggca
     3241 ttgcagccac ggccacgatc cggcgagagc tgcccgatac cgaggtactg gcgctcacca
     3301 gcgtgctgga ggacgcctcc gttgttgggg cagtacgtgc gggggcgatc gggtacttgc
     3361 tcaaggacac cgaggccgat gcgttatgcc gggccatcaa agcggcggtg gagggacagg
     3421 tgcagttgac gccacaggcg gcagcgcgct tgatgcaggt cgtgagcgcg ccagagagtc
     3481 cacagccact taccgaacgc gagaccgaag tcctgcggct gctggcgcag ggccaggcca
     3541 acaagcacat cgcgcgcagc ttactcatta ccgagaagac ggtcaaggcg catgtcagca
     3601 atattctcgg caagctcggt gtgcagagcc gtacccaggc cacgctctac gcgatccgca
     3661 tcggcctggt ctccgcgaat gcccctggaa ccgaatggtg agaggaggag agcagcgatg
     3721 aaccattccc ctgcatcgac tccccttggc gaaaccggcc tgccacgaag cgagacggat
     3781 atgcgcttat ctggctcctg gctggtgctt gcgcaggtcg tatgtctcac cctgtgtgtc
     3841 ttgtcggtgg ggctctttgt tgggagcatc ctgtcgtaca tcgtgaataa ctacttgttc
     3901 tgcacgagca ccgccgctgc ttgctttgcc catgggcagc tgacacccgg cgacgtgcaa
     3961 cggcttcaag aactcggcct ttccaggggt ttcattgccc tctaccccat cgtgctcgat
     4021 agtcttgttt cccttggata cctggtggtc gcggccttcc tcttctggcg caagtcggat
     4081 gaccgggtgg cgctcatggc agccctctcc ctagcgctct ttcccatggt gttcaattat
     4141 ggcttgacga gcgcactgcc atcgccctgg tggttcctgg ccaatgtgct cagctttgtc
     4201 ggctccctcg gcttcattct cttctattac gtgtttccca gtggacactt tgtccccggc
     4261 ttcacgcgct ggctcctggt gatggcgctc gtgtattggg ggctcaatga attctttccg
     4321 ttcccttcct ttaacccgtt ctatcgcttc cacctgctca atggcctgac ctatctcggc
     4381 ctgcttagta gtgccgtcgt tgcgcagctc taccgctatc ggcgggtgtc cagtcctaca
     4441 gagcgcctgc agaccaagtg ggtggtttat ggagaggtaa tgggccaggg agtgtacgtg
     4501 gtgctgttta ccatctcgct gttcgttccg tcgatcttgt cgacgggctc gctggtcagc
     4561 ttgatcaggt tgaccgtcgt ctatgttctc tcgcttctta ccccgctttc catcggcctg
     4621 gcgatcttgc gttccagatt gtgggagatc gacgccatca tcaatcgcac gctggtctac
     4681 ggcatcctca ccgtctgcgt tgtcggggtg tacgtgctgg tggtgggatc gctgggcgcg
     4741 ctcattggcg ccagtggcaa tctgctgatc gagctgctgg caaccggcct ggtggccgtg
     4801 ctgttccaac ccttgcgcgc gtgggtgcaa cggggggtaa accgcctgct cttcggtcag
     4861 cgcgacgaac cctatagcgt catcacgcag ttgagccagc gcctggaggc ccagctttct
     4921 cccgatgctg tattatcgac cattgtggag accgtggccc aggcgctcaa gttgccgtat
     4981 gtggccattc tcttgaagca agaagaaact ttcgttctgg cggcgagcgc tggagaactg
     5041 gttggagatt ctctggacct gccgttggtc taccaggcag agaccattgg tcacatgcgt
     5101 cttgcccccc gcacgcccgg tgaaccgttc actcctgctg atcggcgctt gctggacgaa
     5161 ctggcccgtc aggccggcct ggccgcccat gcgattcagt tgacggcgga cttgcagcgc
     5221 tcccacgaac aattggagca gcgagtagca gagcgcaccc gcgagctctc ctcgctattg
     5281 gacatctccc ataccgtggc ctccacctta caattgaagc ccctgttggg actgatcctc
     5341 gatcaactca agctggtcat cgattacact ggttcctcga tcttaatcat cgagggagaa
     5401 gactttgtgt ttcttgacca ccggggaccg gtcccgcaag atcaactggt acgactccgt
     5461 tttccacttg aagggatggg atcaatctgg caaactattg cctctggtga atcgatcctc
     5521 attgaagacg tgcatgagca gaccggactc gcgcaggcct tgcgtgcggc catgggtgat
     5581 ctcctgacga ccacctttca gtatgtttgc agctggatgg ccgttcctct catcttgcga
     5641 gaccaggtga tcggcatgct ggtgctcact tcaagcgaag agcaggcctt tacgcagcga
     5701 caggcgacgc tcgcgctagc tattgccaat caggcagcca ttgcgattga aaatgcgcgg
     5761 ctctatgaac aggcgcagga actggcggct ctggaggagc ggcagaagct ggcacgcgaa
     5821 ttgcacgatt cggtctcgca ggcgctctac ggcatcgcgc tgggggccca taccgcccgt
     5881 acactcctca accgcgaccc ggccctggtg gcccaaccgc tggagtacat tctctcgctg
     5941 gccagggcag ggctggtcga gatgcgggcc ttgatctttg agctgcgtcc agagtcgttg
     6001 gagaccgagg ggctggtctc ggcgctcctc aaacaggccg cggccttgca ggcgcgccac
     6061 gaggtaccgg tcgagaccga tctgtgcgag gaaccgagcc tgccgctcaa ggtcaagcag
     6121 gaactctacc gggtcacaca ggaggccttg cacaacacca tgaagcatgc cgaggccagc
     6181 caggtaaagg tgaggctagg ccagacgggt gagactatca tcctcgagat ccgagataat
     6241 ggcgtcggct ttgacccggc aggcttcttt cctgggcatt taggtttgct ctcgatgcag
     6301 gagcggatta agaatctagg gggagtgctc tcgattgaga gcattcctgg ccaggggacg
     6361 accatccatg ctcgggtccc ggcccgcgag gtcatccgaa gggaataggc ctcaactacg
     6421 cctggtcccg ctctttacgt ccccgtcccc tccagcgtat ccggcgcagt ggagttatgg
     6481 ctgtatacaa cgtcgttcgt accgcggcat tctgccagag ttggccgaat ccctgcagcg
     6541 gactcagcac ctgatggcct tcgacattga gattgagggc gctggtcggt accctttcca
     6601 ccttgagaac cgagacctcc tccgcccaat aggcagtatc gcgtggctgt ttttctggtt
     6661 caggtgatgg agctgtcatg agcctattcc tttctgcctg cttctttctc ctcgtttcaa
     6721 actttcgcga tgccacccga ttcctgcggg ctgttcattt ggcacccaca cttcctccaa
     6781 agccggacga gcgctgcacg gtcaccagcg tgacatcgtc ctcctgctcc caattggggc
     6841 cggtgaagac ggtcagctgc tgcaccagga cgtcgatcag cgagggggca tcgcgctgct
     6901 cttccaggag tttggtcaac tgaggaaagc caaacatcgc acgctctcgg ttatgggcct
     6961 ccaccaggcc atcgctgtac cacaagacgc tatctccatc cgcgatcatc gtctccttct
     7021 cctcatacgt catgccggcc atcaagccca atggcatccc agtcgcccat agttcgctga
     7081 cccggtcgtg ctgccgctgg tagggttgat cgtgtccagc attagcatag cgaagacacc
     7141 cactcgcggg atcgaggaga gcatacaaac aggtcacaaa catgccgggc agcatttcgg
     7201 ggcaaagcag ggcgttggtc tgctctaaca cctgacccgg ggaggcttcg tgccgcgcca
     7261 ctgaacgcag gatggtacgc gtcgtggtca tcagcagcgc ggctgggatg cctttgccgg
     7321 acacgtcccc aatgacgatg cccagacgcc catcggcaaa cgggaggaaa tcatagaagt
     7381 cgccgcccac cgcccgcgct ggttgatagt aggtggccac ctgccagccg accaactctg
     7441 gcaggtccct gggcaggaag gcccgttgaa tctgctgggc gatgcacagt tcctgttcaa
     7501 ggcgctcgcg ttcaagcgcc tgggcttgtt gctcccgcac caactgcgcg acctggatgg
     7561 ctggagcagc ctggatggcc agctggttga gcagcccacg atcgtcgctg gagtagtcct
     7621 gctcgctctt gcgcgggccc aactggagca ggcccaccag ttccccctgg ctcacgagtg
     7681 gcacggccag cttcacccca tcggcctgca gcgcacgcag cgcaggcgag tccaggtgga
     7741 gccgcacggt ctccactgtg ccatgtgcgg agaggagcaa gcccaccagc gggtctgtag
     7801 gtacaaggat cactggtgac gaatctcccg gcgtggccac ctgggtaggg gacggctcaa
     7861 tccctggggc aggtggccga agccagagcg agccgcgcgt cggctgcatc gtgatctgga
     7921 cgactgccag cagatcagcc gagagtgtgg ccaggtccac ctcgtcacgt tgctgcaagc
     7981 gggttccgaa agccgtcagg gtctgggctg catcataagt gcggcggtag aagcgccgat
     8041 caatgacccg ctggagacga tggcgtaacg gctggaaaag ggccgcgatg gccagggtgg
     8101 agaggacgat aacgacgctg ttgtcctggc tgatgatgcc ccgcaggagt gtttgcaggc
     8161 taagcaccag accaacgtac accagtgcca cggtgcctgt cagcgagccg tagatcaggg
     8221 tgcggttgat aaggacatcg atctcccaca gcctggaact cacggttgcg aagccgagag
     8281 aaagtgggac aagaagcacg aagccgtagg aggcagccaa cttgatcagg tctccaagcg
     8341 agccagtctg gaagagggag ggaatgaaca gagcaagggt aaacagcacc aggtgccctc
     8401 ccacgcccat cgacacgcca tagaccaccc acttggtctg ctggcgctct tcgggactgg
     8461 agacccactg gtagcggtag agctgcgcga cgacgatccc accaatgatc ccgagaaaga
     8521 tcaagccacc gagcacctgg gagggagcca acgggttcaa agaggaagaa ggaaagaatg
     8581 cgtcgaaccc ccaatacacc ggcgccacca ccaggagcca gcgtgtgaag cgggggacaa
     8641 agtgcccgct cggaaacacg caaccgaaga ggacgaggca gaagaagccg aggacgctga
     8701 gaacctgggc caggctccac cagggcgagg gcagtctgtt aatcaagcca tcattgatca
     8761 ccataggaaa gagcgccagg gagaaggcag ccaggagcgc cacccggtca tccgacgtgc
     8821 gccagaagag gaaggtggcg accacccagt aacccagggc agaaagactc ataatcccga
     8881 tagtgtaggt cgcatagaag tccctggaaa ggccaagttc gtgaagccgt cgcacatcgc
     8941 ctgctgccag cagcccatga gtaatacaag tagcagcagc gcccgtgcag atctggtgga
     9001 gaagcgcgtt gaccgacagg atgctctcaa cgaagaggaa gagcgccagc acaacgacga
     9061 cgacccatcc aaccctcgcg agcccgagcc aacgcctgtg caagggtgtg tcagccatat
     9121 gaagtggggt tgtcctcacc gccttgttca atgatgcatt cgacctgttc atctgggtgc
     9181 ctccaaacca gtctgatcac acggaccagc agcctccggc ctcgggaagt tcccttcgcc
     9241 ttccgtacac ggtgcgaatc cgctggtgga tgacttacgc gggatcagcc tggaccgact
     9301 ctttccccaa ccaggcgacg atggacagcc agagcatcca ggcactgagc aagacagagg
     9361 agatgatggt caggtcagcc accatgcacc tcctgtatgt tttgtgtgcc acctgacctg
     9421 tgctctcaaa ctactttccg gcagccccga ccacctcttg ctgctccatg cgccgcagac
     9481 tgatatactg ccagatcaaa aatcccac
//
LOCUS       DASZAK010000723         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_550378, whole genome shotgun sequence.
ACCESSION   DASZAK010000723 DASZAK010000000
VERSION     DASZAK010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550378"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VGT44_12760"
     CDS             <1..477
                     /locus_tag="VGT44_12760"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2581717.1"
                     /translation="EIFEQARPVLEAMGKKENIFHVGPSGAGEVVKIVNNLLVGAIAA
                     STAEALTLGVKAGVDVETMTKIIGVSTGASWQLANQFPLRAFNGSFQPGFMTDLLHKD
                     LGLALELAAENALPAPMAALTRQMYEMARAEGYGRNDYTAVLKVIERMAGVEVRTP"
     gene            495..1433
                     /locus_tag="VGT44_12765"
     CDS             495..1433
                     /locus_tag="VGT44_12765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2581718.1"
                     /translation="MMLLSAYLAAHILFEERYEIIDELGSGGFGLVYKARDILRANNL
                     VAIKEVCLRGLTPRETLDAVSSYQREAELLSQLTHPSLPHLYERILMPEQWCLVMDYI
                     EGETLDEYQKKVPNKRLMLSEVFAIGQQLCEVLEYLHTHQPPIVFRDLKPANIIRAPD
                     GKLYLIDFGIARFFKPGQVKDTIALGSLGYAAPEQYGKIQTTPRADIYALGAVLHQML
                     TGNDPSEAPLRLTPLRGKHATAKTDPGSLTSSMVNVLMESLGKLIDSMLEMDINKRPI
                     SVTLVKVELDDLAAIWSEIVGGFVRPRIPRTSQRHR"
     gene            complement(1499..1867)
                     /locus_tag="VGT44_12770"
     CDS             complement(1499..1867)
                     /locus_tag="VGT44_12770"
                     /inference="COORDINATES: protein motif:HMM:NF023276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3368 domain-containing protein"
                     /protein_id="HEV2581719.1"
                     /translation="MVLNEYQAKAPSTEPDLAQSSWLTVVDDVIIDPTLPLLDVGEAA
                     ALTLAQTIGARLVLLDERKARRIAARMGLQVAGTLAVLLRAKQQGHITTIRPIISQMQ
                     SQGRRFHPDLIAHLLEEANE"
     gene            complement(1968..2204)
                     /locus_tag="VGT44_12775"
     CDS             complement(1968..2204)
                     /locus_tag="VGT44_12775"
                     /inference="COORDINATES: protein motif:HMM:NF015630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0175 family protein"
                     /protein_id="HEV2581720.1"
                     /translation="MQLRLQVPERLTGKNQQQLEALAFEALVVRLYALGELSSGEGAK
                     LLGLTRREFLDLLGQYNVSLFDNQVDLKREANYE"
BASE COUNT          568 a          663 c          669 g          506 t
ORIGIN      
        1 gagatatttg agcaggcgcg ccccgtactg gaagcgatgg gcaaaaagga gaatattttt
       61 cacgtcgggc cgagtggcgc gggcgaggtc gtcaaaatcg tcaacaacct gctggtcggg
      121 gcgattgccg cctccaccgc cgaggcgctg acgctgggcg tcaaagcggg ggtcgatgtt
      181 gagacgatga caaaaatcat cggcgtcagc acgggcgcga gctggcagct tgccaaccag
      241 ttccccctgc gcgccttcaa cggcagtttc cagcccggct tcatgaccga cttgctgcac
      301 aaagacctgg gactcgcgct tgaactggcc gccgaaaacg cgctgcccgc gccgatggcc
      361 gccctgacgc gccagatgta cgagatggcg cgcgcggaag gctatggccg caacgactac
      421 acagccgtat tgaaggtgat cgaacgcatg gcaggagtcg aggtgagaac gccatgagat
      481 gaggagcaag ctgcatgatg cttctttcgg cctaccttgc tgctcatata ctctttgagg
      541 agcgctacga aatcatagac gagctgggtt ctggcggttt tggtctcgtt tacaaggcca
      601 gggacatcct gcgcgcaaat aacctggtag cgatcaagga agtctgtttg cgcggactca
      661 cgccgcgaga gacgctggat gccgtcagct cataccagcg cgaggcagaa ttgttgtccc
      721 aattgaccca tcccagcctg ccgcacctgt acgagcggat tctcatgcct gagcagtggt
      781 gcctggtgat ggattatatc gaaggcgaga cactcgacga gtatcagaag aaggttccga
      841 acaagcgcct gatgctgagc gaggttttcg cgattggaca gcagctttgc gaagtcctcg
      901 aatacctgca tacccaccag ccaccgattg tctttcgcga tttgaaaccg gccaatatta
      961 ttcgcgctcc cgatggcaag ctgtatctta tcgacttcgg tattgcgcgc ttcttcaagc
     1021 cagggcaggt caaggatacc atcgcgcttg gttcgctcgg ctacgccgcc cctgagcaat
     1081 acggaaaaat tcaaacaacg cctcgcgccg atatttacgc cctgggagcc gtgctgcacc
     1141 agatgctcac cggcaatgac ccttcagaag ccccattacg ccttactccc ctacgtggga
     1201 agcacgccac cgccaaaacc gaccccggca gtttgacgag cagcatggtc aatgtcttga
     1261 tggagagcct cggcaagctg attgacagta tgttggaaat ggatatcaac aagaggccaa
     1321 tcagcgttac actcgtcaaa gtggagctag acgacctggc tgccatttgg tcagaaattg
     1381 ttggaggctt tgtccgaccg cgcattccgc gaacatccca acggcacaga taagtagaat
     1441 cttcgccata aagaacgcaa cctgggaagg tcaatcatag ggatgaccag ggctggtatt
     1501 attcgttagc ctcctcgagg agatgggcaa tgagatcggg atgaaatcgc cttccctgac
     1561 tctgcatttg agagataatt ggccggatag tagtgatatg tccctgctgc ttggctcgca
     1621 gcagcacagc aagcgttcca gcgacctgta aacccattcg ggcggcgatc cgacgcgcct
     1681 tgcgttcatc cagcaaaacc agccgtgcgc ctatcgtttg tgccaatgtg agcgctgctg
     1741 cttcgccgac atccaataag ggaagggtag gatcaataat gacatcatct accaccgtca
     1801 accacgatga ctgagccaga tccggttcag ttgatggggc ctttgcctgg tattcgttga
     1861 gtaccatacg aggagcccag accttgccat acaagagtgg aagtaggtca agaagcccaa
     1921 cccccgcgag agtaatgagc ggggtcgtgt tggtcacgac cgggcgttta ttcgtagttg
     1981 gcctccctct tgaggtcaac ctggttatcg aaaagggaga cattgtactg gcccaacaga
     2041 tcgagaaact cacggcgagt caagcccaat agtttagctc cctcccctga agagagttcg
     2101 cccagcgcat agagccgcac cacaagggct tcgaaggcga gtgcctctaa ctgttgctga
     2161 tttttcccgg tgagtcgctc aggaacctgc aagcgcaatt gcattgtaac tgtctccttt
     2221 gttggcatca tcactacgta ccaacaccct cattatacta cacatggccg gggtaagtaa
     2281 tcctgcattc gagtgcggcg ctgctaattt gagcgaaatt caggtaattg ttcatacaat
     2341 ggtgtgaatc atgccatgcg agatggtcat gcccaggacc agcacagctg ccaggccgaa
     2401 ataggc
//
LOCUS       DASZAK010000724         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_161026, whole genome shotgun sequence.
ACCESSION   DASZAK010000724 DASZAK010000000
VERSION     DASZAK010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161026"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VGT44_12780"
     CDS             <1..406
                     /locus_tag="VGT44_12780"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV2581721.1"
                     /translation="VAVVTCMDARLHPARFLGLELGDAHVIRNAGGRVSDDALRSLVI
                     SQQLLGTNEVVVIHHTDCGMLTFSNDDLRAKVKRDLHANADHIDFLPFKDLEQSVRDD
                     VATIKASPLIPDSVQVSGFIYDVKSGKLLPVT"
     gene            complement(417..>2015)
                     /locus_tag="VGT44_12785"
     CDS             complement(417..>2015)
                     /locus_tag="VGT44_12785"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV2581722.1"
                     /translation="WFPFPSNEAIDRAMLANPTYLYVSHLHHDHFDPQFLQDNVSKDA
                     TVILPDYPLNLLERRLRDLGFTKFLQTKNAEAMEVDGLRIMVMAMTAPIDGPLGDSSL
                     VIDDGETRLLNQNDSRPIDIDLLNSFGPFDAHFVQFSGAIWYPMVYQFPEKMMQALGR
                     KKRDNEMARALRYIREIGASYVVPSAGPPCFLDDHLFNFNDFDRNPANTFPDQTVFID
                     YMKVNDRDNGRLMIPGSVGILSKGGFTIEHPLLESQVQAIFTEKRAYLQQYRTRQQPL
                     IDAQKASWPRGQVDILAALREWFEPLMEMADLTAVGINGRVLIDCDVQQIVLDFHTRR
                     VYAWSDEEWDYRFHIDHALLEYCIIHHEEDWINQIFLSCRFEAQRKGAYNEYVYNFFK
                     CLSLERLQYAEGYYAEKTAPHQLWESNGYRIQRRCPHLKADLPRFARIENGVLTCTMH
                     GWQFELATGRCLTSEGHPLYAQPIEQPAVDHSDAAQSNGLEQSYEGATPAAIAEQAGQ
                     FVRTKCDHCWYKETDVKSSKAEAQ"
BASE COUNT          376 a          534 c          657 g          448 t
ORIGIN      
        1 cgtggctgtg gtgacgtgta tggatgcgcg cctgcatccg gcgcgtttcc tcggcctgga
       61 actgggcgat gcccacgtga tacgcaacgc gggcgggcgg gtcagtgacg atgcgttacg
      121 ctcgctggtt atttcgcagc agttgcttgg caccaacgag gtcgtggtta ttcaccatac
      181 tgactgcggc atgttgacct tcagcaacga cgatttgcgc gcgaaggtga agcgcgacct
      241 gcacgccaac gctgaccata tcgactttct gccgttcaaa gacctggagc aatcggtgcg
      301 cgatgatgtg gcgacgatca aggcctcccc gctgatcccg gatagcgtgc aggtgagcgg
      361 ctttatctac gatgtgaaaa gcgggaagct gctgccggta acataaaaaa aagtcgttac
      421 tgcgcctcgg cttttgaaga tttcacgtcc gtttctttat accagcagtg atcgcatttt
      481 gtccgtacaa actgcccggc ttgctctgct atcgctgctg gtgtggctcc ctcgtagctc
      541 tgctcaagcc cgttcgattg tgccgcatca ctgtggtcaa cggcgggctg ttcgatgggc
      601 tgcgcgtaga gggggtggcc ttctgaggtc aggcagcgac cggtggccag ctcgaattgc
      661 cagccgtgca tggtgcaggt caggacgccg ttttcgatgc gagcgaaacg aggcaggtcg
      721 gctttcaggt gcgggcagcg gcgttggatg cggtagccgt tgctctccca gagctggtgc
      781 ggcgccgtct tttcagcgta gtagccctca gcgtattgca agcgttcgag tgagaggcat
      841 ttgaagaagt tgtagacgta ctcgttgtac gcgcccttgc gctgcgcctc gaagcggcac
      901 gagagaaaga tctggttgat ccagtcctct tcatgatgga tgatacagta ctcgagcagg
      961 gcgtggtcga tgtggaagcg gtagtcccac tcttcgtcgc tccaggcgta gaccctgcgc
     1021 gtatggaaat ccagcacgat ttgctgcacg tcgcagtcga tcagtacgcg accgtttatg
     1081 ccgaccgcgg tgaggtcggc catttccatg agcggctcga accattcgcg cagcgcggcc
     1141 agaatatcga cctgcccgcg cggccaggat gccttctgcg cgtcgatcag cggctgctgg
     1201 cgggttctat attgctggag ataggcgcgt ttctcggtaa agatggcttg tacctgtgac
     1261 tccagcaggg gatgttcgat ggtgaaaccg cctttggata ggatgccaac gctgccgggg
     1321 atcatcaagc ggccattatc cctatcattc accttcatat agtcaatgaa gacggtctga
     1381 tcggggaaag tattggcggg gttgcggtcg aagtcgttga agttgaagag atggtcgtcc
     1441 aggaagcagg gcggcccggc ggatgggacg acgtagctcg cgccgatctc gcggatgtag
     1501 cgcagggcgc gcgccatctc gttgtcgcgc tttttgcgcc ccaatgcctg catcatcttc
     1561 tccgggaact ggtagaccat ggggtaccag atcgcgccgg agaattgcac aaaatgggcg
     1621 tcgaaggggc cgaagctgtt gagcaggtca atgtcgatgg ggcgcgagtc gttctggttg
     1681 agcaggcgcg tctcaccgtc gtcgatgacc aggctggagt cgccgagcgg gccatcgatg
     1741 ggcgcggtca tggccatcac catgatgcgc agcccgtcca cctccatcgc ctctgcgttc
     1801 ttcgtctgga ggaatttcgt gaagcccagg tcgcgcaggc ggcgttccag caggttcaac
     1861 gggtaatcgg gcaggatgac ggtggcatcc ttcgagacgt tatcttgcaa gaactgcggg
     1921 tcaaagtgat cgtgatgtag gtgcgaaaca tacaggtagg tcgggttcgc cagcatcgcg
     1981 cggtcgatgg cttcgttgct ggggaaaggg aacca
//
LOCUS       DASZAK010000725         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_301945, whole genome shotgun sequence.
ACCESSION   DASZAK010000725 DASZAK010000000
VERSION     DASZAK010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301945"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..621
                     /locus_tag="VGT44_12790"
     CDS             43..621
                     /locus_tag="VGT44_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581723.1"
                     /translation="MAFTINPEQVASYEAAGWRAYYDRKWLKMLRLILQLVQEQFHIP
                     FPMSLFASYYTARASQLWAPVNHDEQKVLAYLTKFYGVARRYSGLRFDVEEVARLELQ
                     YFEVHRRLSGQPEKEEFLQTLVALHSAIFALSPEAVRESAEWRLKAANIVDLITSRTS
                     SNIEEDWKALEEYLRRCYAAIQKALDAAASAR"
     gene            complement(608..1348)
                     /locus_tag="VGT44_12795"
     CDS             complement(608..1348)
                     /locus_tag="VGT44_12795"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEV2581724.1"
                     /translation="MSTDTPEISAGSVAHHFRYTRLLATSLDVYTDSVPAASALSVRP
                     PLAVDPVANFSRAVRRAMHERFQHIFLQLTPRMKAKDPQLYTHSQRVLSLTLRLLRIL
                     DLSQDEIVSIALAAFFHDIGKLYLDDALLAKTTPLTPDEYGMIQQHSAFGVDLLAPYL
                     TSQLSTLSFVYHHHERWDGQGYPCGLEGEAIPLGARIIAVADAFDAMTSERVYQAPRT
                     PYEALLELVRCAGTQFDPRLVRLFLIVR"
     gene            1606..2232
                     /gene="rdgB"
                     /locus_tag="VGT44_12800"
     CDS             1606..2232
                     /gene="rdgB"
                     /locus_tag="VGT44_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEV2581725.1"
                     /translation="MRTLLIATTNRHKLEEIQTILCDVPFQLCSLRDLGVEMDVEETG
                     QTFAENATIKALAYAQVTGLLCLADDSGLEVDALGGAPGIYSARFLGQGTPYEERFRF
                     ILEKMQGLTGPQRSARFRCSMALAEPTGECRVVEGTVEGEISDSPRGAFGFGYDPIFL
                     VPGLEKTTAELRPEQKNWISHRGRAAQLARILLQDWPDPIVHRGANDA"
BASE COUNT          512 a          586 c          709 g          467 t
ORIGIN      
        1 gctcatgagg agtgaatcgt aacaaggaga gataagaggt acatggcatt cacgatcaat
       61 ccagagcaag tagcctccta cgaggcggcg ggatggcgcg cctattatga tcgaaaatgg
      121 ctcaagatgc tgcgtttaat cctgcaactc gttcaggagc agtttcatat tccttttcca
      181 atgtcgctct tcgcctccta ttatacggcg cgcgcctcac agctttgggc gccggtgaac
      241 catgatgaac aaaaggtgct ggcctacctg acaaagttct atggggtggc gcgtcgctat
      301 tcgggtctgc gcttcgacgt agaggaagtc gcgcggctgg aattgcagta ttttgaggtg
      361 catcggcgac tatcaggcca accggagaag gaggaatttt tacagacgct cgtcgcgctg
      421 catagcgcca tcttcgcgct ttcaccggag gcggtgcgcg agtctgcgga atggcgcttg
      481 aaggcggcaa acattgtcga tctcatcacc agccggacat ccagcaacat cgaggaagac
      541 tggaaagctt tggaagagta tttacgccgc tgttacgcgg cgattcagaa ggcgctggac
      601 gcggctgcta gcgcacgatg aggaagagcc gtaccaggcg cgggtcaaac tgcgttccgg
      661 cgcagcggac aagctctaga agcgcctcgt agggggtgcg cggcgcctgg tagacacgct
      721 cggaggtcat ggcgtcaaag gcatcggcga cggcgatgat acgcgcgccc aggggtatcg
      781 cctcgccttc cagaccgcag gggtagccct ggccatccca ccgctcgtgg tggtggtaga
      841 cgaaagacag cgtggagagc tgcgacgtca gatagggagc aagcaggtca acgccaaagg
      901 cggaatgctg ctgaatcatg ccgtattcgt cgggagtgag cggcgttgtt ttcgcgagga
      961 gcgcgtcgtc aaggtagagt ttcccgatat cgtgaaagaa cgcggctagc gcaatcgaca
     1021 caatctcatc ctgtgagaga tccaggatac gcagcaggcg cagcgttagc gacaagaccc
     1081 gttgagagtg cgtgtacaac tgtgggtctt tggctttcat acgcggcgtc aattgcagga
     1141 agatgtgttg aaaacgctcg tgcatcgcgc ggcgcacagc gcgtgaaaaa ttggcgacgg
     1201 gatcaaccgc gagtggcgga cgaaccgaga gcgcagaggc agcaggcacc gagtcagtat
     1261 acacatcgag agaagtggca agcaggcgcg tgtaccggaa gtggtgagcc acacttccgg
     1321 cgcttatttc gggcgtatcg gtactcatag cccagctctc tttcgtatag ccaaacggtc
     1381 acttagatga tagagccttc tatgtagata gacgttagcg ggtggcattt ctcgcggttt
     1441 catgcatcgc aaccgtctac taaatataat acgtcatcgc gtgacaatat gagtcgtttc
     1501 cctgccaata gtcctatctg cgggcgcgat aataactcga caacaggcac tcgatatgat
     1561 aagatagggt tcattgcaga tttttgcgta attgaaagaa agtgtatgcg gacgttgttg
     1621 atcgcgacca ccaaccgcca caagctggaa gagattcaaa cgatactttg tgatgtgccg
     1681 tttcaactct gttcgctacg tgatctaggg gtagagatgg atgtcgaaga aacggggcag
     1741 accttcgcgg aaaatgccac catcaaggcg ctggcctatg cgcaagtgac tggcctgttg
     1801 tgcctggccg atgactctgg cctggaggta gatgctctgg gaggtgcgcc gggtatctac
     1861 tcggcgcgtt ttttgggcca gggcacaccc tatgaagagc gttttcgttt catcctggaa
     1921 aaaatgcagg gattgaccgg gccgcagcgc agcgcccgct tccgctgctc aatggcgctg
     1981 gccgaaccaa ccggagagtg ccgcgtcgtc gaaggaacgg tcgagggaga gatctcggac
     2041 agtccgcgtg gcgcgtttgg tttcggctac gacccgatct ttctcgtgcc tggcctggag
     2101 aagacgaccg ccgagttgcg gcccgaacag aagaactgga tcagccaccg ggggcgcgcg
     2161 gctcaactgg cgcgcatcct gctgcaagat tggcccgacc cgatagtcca tcggggcgca
     2221 aatgatgcgt aggggcgcaa ttcattgcgc cttcgtcccc ggcaaggcgc attc
//
LOCUS       DASZAK010000726         1979 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_516632, whole genome shotgun sequence.
ACCESSION   DASZAK010000726 DASZAK010000000
VERSION     DASZAK010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516632"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VGT44_12805"
     CDS             complement(<1..455)
                     /locus_tag="VGT44_12805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2581726.1"
                     /translation="MAEQAGYVGQRLGNYVLVRLLGQGGFAEVYLGEHIHLKTQAAVK
                     LLHTRLAHDDIDGFRREAQTIARLVHPHIVRVLDFGVEGSAPYLAMDYAPYGTLRNRH
                     PKGARVPLPAVTQYVRQAAEALDFAHAQRLIHRDVKPENMLVGRNGEILL"
     gene            complement(628..1791)
                     /locus_tag="VGT44_12810"
     CDS             complement(628..1791)
                     /locus_tag="VGT44_12810"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV2581727.1"
                     /translation="MRRVFHMIGAITLLLSIMLVGGAGTTLAASRWDKDNGQFQHIFV
                     IMMENHSANEIFGNTADAPFINQLTSYAAVSTDYFGVTHPSSPNYLAAISGDFQHIWD
                     DCAAGPTVTCAPEEFGPTSGYTNGQEWLTPQEIAQATNTPHWFSDQTIVDQLEAHGLS
                     WKAYMQSMPYTGFTGATYPNPVVNGKVVPVSLYVQKHNPFDYFPAIVNNPDRMQKVVP
                     FDQFDGDLDSGNVPNFVWISPDTCNDMHGISSTGAALINQPNCAYPASGLDHSIIKLG
                     DNYLRNTVNEITESSVWQNTRSAIVIMWDENDYSSANGCCHSPTGVNGVVLGGSSAPA
                     LVLTSRDQEHFTDSTDNYNHYSLLGTIEKLWDLGCLENSCGFSGSQLMTKFFE"
BASE COUNT          435 a          541 c          580 g          423 t
ORIGIN      
        1 agcagaatct caccgttgcg cccaaccagc atgttctccg gcttgacatc gcggtggatc
       61 aaacgctgcg cgtgcgcgaa gtccagcgcc tccgccgcct gccggacata ttgcgtgacc
      121 gccggcagcg gcacgcgcgc gccctttgga tggcggttgc gcagcgtgcc atagggggcg
      181 taatccatcg ccaggtaggg cgcgctgccc tccacgccga agtccagcac gcgcacgata
      241 tgggggtgaa cgagccgcgc aatcgtctgc gcctcgcgcc gaaagccatc gatatcatcg
      301 tgcgccagcc gcgtatgcag cagcttcacg gcggcctgcg tcttgaggtg aatatgctcg
      361 cccaggtaca cctccgcgaa acctccctgt cccagcagtc gcacaagcac atagttcccc
      421 aggcgttgcc cgacgtagcc ggcctgttct gccatatctg cttttactac ccctcataga
      481 aaaaagtcgc tgtattgcac aagctgagtg taggggaaag caagggagca tgtcaaaaga
      541 ataggcagga tgtcttatca agacgaacga acgaggcgga ttccacaacg tgaaatccgc
      601 ctcgttcggt gatgtcggtg agcgacttta ctcaaaaaac ttcgtcatca actgcgaacc
      661 gctgaaaccg caggagtttt ccaggcagcc caggtcccac aacttctcga ttgtgccaag
      721 cagcgagtag tggttgtagt tgtcagtgct gtcagtgaaa tgctcctggt cgcgcgaggt
      781 gagcaccagg gccggggcgc tcgatccgcc caggacgacg ccgttgacgc ccgtcgggct
      841 gtggcagcag ccgttggcgc tggagtagtc gttttcgtcc cacataatca cgatggccga
      901 gcgagtgttt tgccacacgg aagattcggt gatctcatta accgtgttcc gcaagtagtt
      961 gtcaccgagc ttaatgatgc tgtgatccag gccggacgcg ggatacgcgc agttcggctg
     1021 gttgatgaga gccgcgccgg tcgacgaaat gccatgcatg tcgttacagg tgtcggggct
     1081 gatccagacg aagttgggga cattgcctga gtcgagatcg ccgtcaaact gatcgaatgg
     1141 cacgaccttt tgcatgcggt ccgggttatt gacaatggcg gggaagtaat cgaacgggtt
     1201 atgcttctgt acgtacaggc tgacgggtac aaccttgccg ttgacgacag gattgggata
     1261 ggtcgccccg gtgaagccgg tatacggcat cgattgcatg tacgccttcc agctcaaccc
     1321 atgcgcctca agctgatcga cgattgtctg atcgctgaac cagtgcggcg tattggtcgc
     1381 ctgtgcgatt tcttgcggcg tcaaccattc ctggccgttg gtatagcccg atgtcgggcc
     1441 aaattcctcc ggcgcgcatg tcaccgtagg ccctgctgcg cagtcgtccc agatatgctg
     1501 gaaatcgccc gaaatggcgg cgaggtaatt cggcgaactg ggatgcgtca cgccaaagta
     1561 atcggtcgag accgccgcgt agctggtcaa ctggttgata aatggcgcgt cggcggtgtt
     1621 cccgaaaatc tcattggccg agtggttctc catcataatc acaaagatat gctggaattg
     1681 accgttgtcc ttgtcccatc gtgacgccgc cagggtagtg ccagcgcctc ctacgagcat
     1741 gattgacagc agcagtgtga ttgccccaat catgtgaaat acacgtcgca taaagcctcc
     1801 tgcatgtgta gaatgaaaag agacacgaaa agaggaggaa tccttttccc cttatatcag
     1861 ggtaatcgat gaaggttatt gaactgtaaa ggggatgtta aatttggcca ggtgatttac
     1921 ggcgaagaga agaagaaaag gcatattaca accatccctt ttgttcggcc aggtaggcc
//
LOCUS       DASZAK010000727         1984 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_161162, whole genome shotgun sequence.
ACCESSION   DASZAK010000727 DASZAK010000000
VERSION     DASZAK010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161162"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            328..786
                     /gene="ruvX"
                     /locus_tag="VGT44_12815"
     CDS             328..786
                     /gene="ruvX"
                     /locus_tag="VGT44_12815"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV2581728.1"
                     /translation="MSRIPGEPSAGRVLAIDYGRKRLGLALSDALGVTARPLATWSRS
                     KRRQDMARLRSLCREQEIKQIVVGWPLQLSGSHGEMAAEAARFAERVHKELSIPVELI
                     DERLSSWEARQALEESAGKARSRNRGQHELDEVAAAVILRDYLRRTSGTT"
     gene            795..1874
                     /gene="mltG"
                     /locus_tag="VGT44_12820"
     CDS             795..1874
                     /gene="mltG"
                     /locus_tag="VGT44_12820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV2581729.1"
                     /translation="MRKAFTLLVLAAMVVAGWVGMSLYLPYQNFPSQGVFVDVPHGAS
                     RRTVARLLASQGVVRSRLVFEGLSRWRSRSTLQAGEYFFDHPITAVQAFDTIANGRVY
                     VRELTIPEGFSMFDIADLVAREDFTTRESFLAAARNPALIRDLIPGAPSLEGFLFPAT
                     YEFPRHPTGEQMVSAMVTHFRDEWTKISAEQTNVSGLPLQQVVTLASLVERETPRPEE
                     RPVVAGVFLNRLHHRIPLQCDPTVVYALELAGSYGGALDAKSLPFNSPYNTYRHIGLP
                     PGPIANPGSASLKAALNPPDTDYLYFVANTEGGHFFGKTLAEHNHNVALYRQRLAEQR
                     SAIPGNGSTVPPRPHTVSAAPQGSR"
BASE COUNT          417 a          544 c          616 g          407 t
ORIGIN      
        1 atggggaacg gccgcagcag taaccacggc gattttgaat ggcgcgcata ttgttcgcgt
       61 tcacgacgtg aacgagatgg cgcaggtggc gcggatcgcg gatacggtgc tttcggcagc
      121 caaaccgcgc ggtgagcgca aaagatcggg tgggctataa tccgatagca gcgaaattcg
      181 aattctgcgc agctgagggc gcggcacatc tggccaaatt gggagagcgg ccgacggggc
      241 cgtaaccccc agaaaccaca tgggattgaa aaaggattag tccctacgta agggcttata
      301 atcaaagtta tgggtttgcg tgtggtcatg tcccgaattc cgggtgaacc aagcgctggc
      361 cgtgtgctgg caattgacta cggtcgaaaa cggttgggac tggccctgtc cgatgcgcta
      421 ggcgtgactg cccgccctct ggcgacctgg agccgctcaa agcggcggca agatatggct
      481 cggttgcgca gcttgtgccg ggaacaagaa atcaaacaaa tcgtggtggg gtggcctttg
      541 cagcttagcg gttctcatgg ggagatggct gcggaagctg ctcgctttgc ggaacgcgtc
      601 cacaaggagt tgagcattcc cgtcgaactg attgacgaac gactgagtag ctgggaagcg
      661 cgacaggcac tcgaggagag cgccggcaag gctcgatctc gcaacagagg gcagcatgaa
      721 ctggatgagg tggctgctgc cgtaatcctg cgcgattatc tgcggcgcac gagcgggaca
      781 acttaggatt acgcatgcgc aaagctttca cactactggt attggccgcc atggttgttg
      841 ccgggtgggt gggaatgtca ctttaccttc cttaccagaa cttcccgtca cagggtgttt
      901 tcgtggacgt gcctcacggc gcatcgcgac gcactgtcgc ccgcctgctc gccagtcaag
      961 gcgtagtgcg cagccgtttg gttttcgaag ggctgtccag atggcgctcg cggagtactt
     1021 tgcaggccgg cgaatatttt ttcgatcatc ccattacagc tgtccaggcc tttgacacga
     1081 ttgccaacgg gcgcgtgtac gtccgcgagt tgaccatacc ggaaggcttt tcgatgtttg
     1141 acatcgcgga tctggtggcg cgggaggact ttaccaccag agagagcttc ctggcggccg
     1201 cacgcaatcc ggcactgata cgagatctca ttcccggagc gcccagcttg gaaggatttc
     1261 tgtttcctgc gacttacgaa tttccacgtc atccgaccgg cgaacagatg gtctccgcca
     1321 tggtgactca cttccgcgac gaatggacaa agatcagcgc tgagcaaaca aatgtgtcgg
     1381 ggctgccgtt acagcaggtg gttacgcttg cgtctttagt cgagcgcgag acgcccaggc
     1441 cggaagagcg gccggtggtc gccggggttt ttctcaatcg cctgcaccac cgcatacctt
     1501 tgcagtgtga tccgactgtg gtgtacgcgc tggaattggc tgggagttat ggcggcgcgc
     1561 tggatgcgaa gagcttgcct ttcaactcgc cctacaacac ataccggcat atcgggctgc
     1621 ctcccggtcc gatcgcgaac ccgggcagcg catcgttgaa agccgctctg aatcctccgg
     1681 acaccgatta cctctatttt gtggccaaca cagaaggagg ccatttcttc ggcaagacac
     1741 ttgcggagca taatcataat gtggctctct accggcaaag gctggccgag cagcgtagtg
     1801 ccatccctgg aaacggttct acggtcccgc cgcggccgca taccgtgtcc gcggcccctc
     1861 agggctcgcg atgagcacac cgggcgcgga atccaaaaag caagccatcc tggaaatcgc
     1921 tgaaatactg gggcttgaaa agattacccc ggcggaaata gagcagatca ggcggcagct
     1981 cgtg
//
LOCUS       DASZAK010000728         3855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_302020, whole genome shotgun sequence.
ACCESSION   DASZAK010000728 DASZAK010000000
VERSION     DASZAK010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302020"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..2571
                     /gene="topA"
                     /locus_tag="VGT44_12825"
     CDS             136..2571
                     /gene="topA"
                     /locus_tag="VGT44_12825"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV2581730.1"
                     /translation="MTKKLVIVESPAKAKTIEKFLGRDYEVRASMGHIIDLPKKGLGV
                     NTRKDFAPQYEVIEKKGKLIDELKAASRRADEVYLAPDPDREGEFIAWSLKETLGLQH
                     PRRAFFNEITREAVQQAIRKPREIDEDLFNAQQARRVLDRLVGYKISPLLWRRIQSGT
                     SAGRVQSVALKLVCDREAEIQAFVSEEYWSITALLSKQGQAELFEATLITRLKDMANL
                     SSEDRVGNGDGENGADSAADAAQMNAKNGAKAARVEKGRIKIASKDEADAILADLNGA
                     IYTVLKLEEREQRRQPYLPYTTSTMQQDASIRLFIKPKKTMSLAQQLYEGIELGERGH
                     QGLITYMRTDSTRISEEAQQSVKAYIAHEFGQPYVGTGRTGKAKATTQDAHEAIRPTD
                     VSLTPQAVKSHLTPDQFKLYNLIWRRFVASFMSPAIFDTVRVDIGAAQYVFRATGSHL
                     KFQGFYAVWPREEDEKMLPALAVDERLDLRELKPEQHFTQPPPRYNEASLIKELEEQG
                     IGRPSTYVPIISTIQDRGYVDQEQRRFIPTWLGGTVNEVMNKHFPDIVDVGFTADMER
                     KLDDVEEGRQSWTEFLRDFYGGFKTSLEKAEAEMNRVQKPIEELDELCPECGRNLVIR
                     TGRFGRFISCSGFPECRYGRTIVTKTGAFCPQCGGDLVERKTRQKKRIFYGCNNYPTC
                     NFAIWERPVPELCPKDGGLMVVARAGADPVCYLEVIVPQRAAEEKPQQDGTTARRTTR
                     RSTSAATTATKKTTGVRKTAATKKTATAKRASGTVAGTKKATGTKRAAKTTTRRASSS
                     ARKTTTTARKKTS"
     gene            complement(2728..3516)
                     /locus_tag="VGT44_12830"
     CDS             complement(2728..3516)
                     /locus_tag="VGT44_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581731.1"
                     /translation="MTTSNTSSNISSKAQKEIIKIVPLYTPEITRASMSAPQLVYNNG
                     PLLTAVQVVLVFWGSAWQQSPLSDTAQSMSDFFDAMLTSSLIDQLAEYNTPQQQIGHG
                     SRIGNYTITSPDPGTSVADSAIQQIISDELANNNTFPQPTANTLYFVLLPTGTAVQQG
                     GSSSGQTFCGYHDSFSDQSGQIYYAVIPQPDCSGCAASLATLDALTEISSHELCEAIT
                     DPIPGQGWYDQNNGEIGDICAWQTKQIGTYTVQKEWSNKANACV"
BASE COUNT          908 a         1010 c         1153 g          784 t
ORIGIN      
        1 gatatgttat cctatgctgt gaggtggctt ggagataaat ggagagacct ggggcgcttg
       61 aagtgtgtaa tgaagctgct tctgaccgga agcgcgggca gcagccaacc aaatgaaaat
      121 atagggtaaa agggaatgac gaagaaactt gtcattgtag aatcgccggc aaaagcgaaa
      181 accattgaga aattcctggg gcgtgattac gaggtgcggg cctcgatggg acatatcatc
      241 gacctgccca aaaagggtct tggcgtcaat acgcgcaagg atttcgcgcc gcaatacgaa
      301 gtgattgaga aaaaaggcaa actgattgat gaattgaagg ctgcgtcgcg tcgtgccgat
      361 gaagtctacc tggcgccgga ccccgatcgt gagggggaat ttatcgcctg gtcgctcaaa
      421 gagacgctag gattgcagca tcctcgtcgt gcctttttca atgaaataac cagggaagct
      481 gtacagcagg cgatccgtaa gccgcgcgag atcgatgaag acctgttcaa cgcgcagcag
      541 gctcgccgtg tcctggacag gctggtcggg tacaagatca gccccttgct ctggcggcgc
      601 atccaatcgg gaaccagcgc cgggcgcgtc caatcggtcg cgctcaagct ggtctgcgac
      661 cgcgaagcgg agatccaggc atttgtctcc gaggagtact ggagtatcac cgcgctgctg
      721 agtaagcagg ggcaggccga gctattcgag gctacgctca tcacgcgcct gaaagacatg
      781 gcgaacttga gcagcgagga ccgggtcggc aatggtgatg gcgagaacgg cgcggattct
      841 gccgccgatg cggcgcaaat gaatgcaaag aatggcgcga aagctgcgcg cgtggaaaag
      901 ggccgcatca agattgcctc gaaggacgag gccgacgcga ttctggccga cctgaacggc
      961 gcgatttata cggtattgaa actggaggag cgcgagcagc gccgccagcc ctatctacct
     1021 tatactacca gcacgatgca acaggatgcc tcgatacgac ttttcatcaa gccgaagaaa
     1081 accatgagcc ttgcgcagca actttacgaa ggtatcgaac tgggcgaacg cggccaccag
     1141 ggcttaataa cctatatgcg tactgattcg acgcgcatct cggaggaggc gcagcagagc
     1201 gtcaaagcct atattgcgca cgagttcggg caaccgtatg ttggcacggg gcgcacgggc
     1261 aaggcgaaag caaccacgca ggacgcgcac gaagctattc gcccgaccga tgtgtctttg
     1321 acgccgcagg cggtcaaatc gcacctgacc cccgatcagt ttaagctgta taacctgatc
     1381 tggcggcgat ttgttgccag ctttatgtcc ccagccatct tcgacacggt acgtgtggat
     1441 atcggcgcgg cgcagtatgt ctttcgcgct accggctcgc atctaaaatt ccagggattc
     1501 tatgccgtct ggccgcgcga ggaagatgaa aaaatgttgc ccgcgctggc agtcgatgag
     1561 cggctggact tgcgcgaatt gaagccggag cagcacttca cgcagccgcc tccgcgctac
     1621 aacgaggcca gtctcatcaa ggaactggaa gagcagggca tcgggcgacc cagcacctat
     1681 gttccgatca tcagcactat ccaggatcgc ggctatgtcg atcaagagca gcgccgcttt
     1741 atcccgacct ggctgggcgg aacggtcaac gaggtcatga ataaacactt ccctgatatc
     1801 gtcgacgtcg gcttcacagc agatatggag cgcaagctgg acgatgtcga ggaagggcgg
     1861 caatcgtgga cggaattcct gcgcgatttc tatggcggtt tcaagacctc tttggagaag
     1921 gccgaggccg agatgaaccg cgtacagaag cccattgagg aattagacga actatgtccc
     1981 gaatgtggcc gcaatctcgt tatacgtaca ggccgtttcg ggcgcttcat ctcctgctct
     2041 ggcttcccgg aatgccgcta tggacgcacc atcgtgacaa aaaccggcgc attctgcccg
     2101 cagtgcggtg gtgatctcgt ggagcgcaag accaggcaga aaaagcgcat cttctacggc
     2161 tgtaacaact atcccacctg caactttgcc atctgggagc ggcctgttcc tgagctttgt
     2221 ccaaaagacg gcggcctgat ggttgttgct cgcgcaggcg ctgaccctgt ctgttacctg
     2281 gaggtaattg ttccgcagcg cgccgcagaa gaaaagccgc agcaagatgg cacaactgct
     2341 cgccgcacga cccgcagaag caccagcgct gcgacgacgg ccacgaaaaa gacgaccggc
     2401 gtgaggaaaa cggcggccac gaaaaagacg gcaaccgcaa aaagagcgag cggaacggtc
     2461 gccgggacaa agaaggcgac gggtacaaaa cgcgcagcga aaactacgac gcgccgtgca
     2521 tcgagttccg cccgcaaaac cacgaccacg gcgcgcaaaa aaacgtcgta gggacgcgct
     2581 gatgaacact cccaaccaaa gtcggttctg tcatgctgag cgcagcgaag catctgtcgg
     2641 cccctcgacc gagacccttc gctgcgctca gggtgacaat cccaaacttg gtcggtaaac
     2701 ttcatcatcg cgtgacaacg acattcctca cacgcaggcg ttcgccttgt ttgaccattc
     2761 cttctgaacg gtataggtcc ctatctgttt ggtttgccat gcgcagatat cgccgatttc
     2821 accattgttc tggtcgtacc atccctgtcc gggtattgga tcggtgatgg cttcgcagag
     2881 ttcgtgcgag ctgatctcgg tcaaggcgtc gagcgttgcc aggctcgcgg cgcaaccgga
     2941 gcaatcgggc tgggggatga cggcatagta aatctgcccg ctctggtcgc tgaaggagtc
     3001 gtgatagccg cagaaggtct ggccggaact cgatccgccc tgctgcacgg ctgtcccggt
     3061 tggcaggagg acgaagtaga gcgtgttcgc ggtgggctgt ggaaacgtat tgttgttggc
     3121 gagttcgtca ctgataattt gctgtatagc gctatccgct acacttgtgc ctgggtccgg
     3181 tgaggtgatc gtatagttgc cgatgcggct gccatggcct atttgctgct ggggcgtgtt
     3241 atactctgcc agttggtcga taagcgagct ggtcagcatg gcgtcgaaaa agtcgctcat
     3301 gctttgcgcg gtgtcgctca ggggcgattg ctgccaggct gaaccccaga agaccagcac
     3361 aacctgcaca gcggtgagca gtgggccgtt gttgtagacc agttgtggcg cggacatgga
     3421 cgccctggtt atctccggtg tatacagtgg cacaatcttg atgatttctt tctgtgcctt
     3481 tgaacttatg ttggacgatg tattgcttgt cgtcacgaat agttcctttc caggtaacga
     3541 attacgcatt tctgcctata acacaaattc ggagccaata tgcagcatag gacgggaata
     3601 tcaaagaagc gacaggatac tgtaaaatat ctgtaaattc ctgcgggtgc agaggtattt
     3661 gttaattctg agtaaaaggc atacaataga tgcattacta ctgtcgcaga agtgccgaca
     3721 gacatgttga aagactctgc tggttttgga ggattgcatg ttacatgcag tgacgctcat
     3781 tcctggcgat ggcacggggc cggagattac cgaggcgacc cggcgggtgc ttgaagcaac
     3841 gggagtgaag ttcga
//
LOCUS       DASZAK010000729         9305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_693814, whole genome shotgun sequence.
ACCESSION   DASZAK010000729 DASZAK010000000
VERSION     DASZAK010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9305
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_693814"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..1857)
                     /locus_tag="VGT44_12835"
     CDS             complement(250..1857)
                     /locus_tag="VGT44_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEV2581732.1"
                     /translation="MGIIESRVERTSPEFQENERYFQGLLAQLNERVRQAQQGGGENA
                     IAKHRKRNKLLARERIQLLCDPETPFLEFSPLAAWDMYENEAPSAGIVTGIGVVEGQE
                     CVIVANDATVKGGTYYPITVKKHLRAQEIAEQNHLPCIYLVDSGGAFLPLQAEVFPDR
                     EHFGRVFYNEARMSSQGIPQISAVMGSCTAGGAYIPAMSDETVIVRGNGTIFLAGPPL
                     VKAATGEEVSAEELGGADVHSRISGVTDHYALNDEHALAICRSIVSNLNRRKYIPWDI
                     HPPEPPLYDPADLYGIVPSDNRKSYDVREIIARLVDGSRFHEFKALYGTTIVCGFARL
                     MGYPVGIIANNGILFSESALKATHFMELCGQRKTPLIFLQNITGFMVGKQYENGGITK
                     DGAKMVMAVANVPVPRFTVITGGSFGAGNYGMSGRAYSPRQLWMWPNARISVMGGEQA
                     ASVLATVRKDGLAARGLRMSEEEEAEFKQPILDKYEQEGNPYYSTARLWDDGIIDPRD
                     TRTVLALGIAASLNAPMPETPFGVFRM"
     gene            2557..4302
                     /locus_tag="VGT44_12840"
     CDS             2557..4302
                     /locus_tag="VGT44_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2581733.1"
                     /translation="MKLPLKQYLKLLAHYLRPQRTRALLMSALLLVSIGLQLANPQVL
                     RYFIDTATGGGNPSLLLLAAALFIGIAVINQAVSIYSRYLSENVAWTATNGMRADLLA
                     HCLSLDMAFHKEHTPGELIERIDGDVNILSNFFSAFVVYLLGNAILLVGILLFLLQID
                     WRVGLALAIFVLLALFILISIRGRIIPLWTADRQMDAEFYGFLGEQLAGTEDIRANGA
                     TAYIMRRFHLLLNRWYPIHRKAGMGYFAMGMTTLTLFAIGNALAFALGAYLWSINKIS
                     LGTVYIIFYYTNLLTQPIDQIRFQLQDLQKAGASIQRVEALLLLQPDITDGKGTPLPE
                     GALSVVFEHVSFGYTKDTTVLEDITFSLQANRILGVLGRTGSGKTTLARLLLRMYDPQ
                     GGQIQLGDVPIAEPRLADLRRHVGMVTQDVQLFHASVRDNLTFFDHAIPDERILAVMD
                     DLGLMPWYRTLPDGLDSELGANGEGLSAGEAQLLAFTRVFLANPGLVILDEASSRLDP
                     ATEHLIEQAVSKLLRGRTGIVIAHRLGTIKRADEILVLENGRILEYGGRAALASDHAS
                     RFASLLQTGLEEVTI"
     gene            4299..6137
                     /locus_tag="VGT44_12845"
     CDS             4299..6137
                     /locus_tag="VGT44_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2581734.1"
                     /translation="MTTFQFLWRMIRYRPWLYLLNCILWTLIHLSPLIPGLIALEFFN
                     TLAASAHLSLALWGLIALLVMTALARVAIFLCGGLADILHRFTMSALLRRNLLERILE
                     RPGARAVPYSVGEAISRFRDDAELAEDAISWSLDTIGEACFASVAVSILVMISAKITL
                     LVFLPLVSVIAIVQALSNRLERYRAASRKATGQVTGMIGETFGMAQAIKVAGAERHVV
                     KHFEGLNEQRRVAMLKDAVLTQMLDSTFENTVGLGTGLILILAAQSLGSANFSVGDLA
                     LFIYYLAFVTDFSHLFGMMLAHYRQTKVSFERMVTLLQGAPPASLVKYEPLYLTRPVP
                     EDGHTLDGHTPDAHKGHPYIWDDPPSQPQIVGVDILVETDGLMYRYPDTGRGIEDVSL
                     RLKRGALTVVTGRIGSGKTTLLRALLGLLPKDVGEIRWNGAPVDDPASFFVPPRSAYT
                     SQIPQLFSETLQENILLGLPEARVDLQAAVEMAVMEQDIAELEQGLATMIGTKGVKLS
                     GGQAQRAAAARMFVREPELLVCDDLSSALDVETERTLWERLFARRANASTCLVVSHRR
                     PVLQRADHIIVLKDGRVEAEGTLDELLQTCEEMRRLWSGEVNANAR"
     gene            complement(6277..6948)
                     /locus_tag="VGT44_12850"
     CDS             complement(6277..6948)
                     /locus_tag="VGT44_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581735.1"
                     /translation="MQKFPIALEIGPKRRVFAQALEWIGWCRSGKDETVALGNLVTTA
                     QRYALVAGAARLAFIPPSSIEDFEVVERVPGTATTDFGAPSVLLASDQEPLEVGDIER
                     LAGLLTASWTTFDDIFICIPPEAHGVKPERGRSPDAMRLHMLEADLMHLSAFGPAFRH
                     PDPARVAEQETSVREQILAQLRAVPCGQTVAPLRRYGFAWMPRFAVRRSAWHALDHAW
                     ELQDR"
     gene            complement(6961..>9305)
                     /locus_tag="VGT44_12855"
     CDS             complement(6961..>9305)
                     /locus_tag="VGT44_12855"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2581736.1"
                     /translation="TLDGRFVSITVAAEFREDGVFRGPQIIIAQSTLDNTPGPGGAKE
                     PAQYGVVYMTMPASYVNAVKAQLSQQYPSARVITANDLLQQRQAQVDQIRLFLRIVGL
                     LALFIGGIGIINTVQVLLRRRQVEIAMLKTTGYRQRDLYALFGLETALLGIAGGVAGS
                     LAGLGISYLVRGVVEQAFFIHLPIIVDPLTLVSGLLIGLSTALIFGLLPIVQAGQVRP
                     LAVLREVIEQRRFSSRLLTLGLLLLLSLLFVALASGILGDVPTAIIAVYGGAGVIFSL
                     ALGFGLLVVAISRLPVYEKPRPRILLSILLALGILLLAALIFGGLALLGEGANMLATR
                     LGNSLVGIYALVVLAGVGIILVGGSLVYLLATIVNGLVAFAPRSWKTAVMLAYRNLGR
                     QRLRTTTTLTALFVGVFAIGLILLLGQGIKDAINSELSTLFTHNLFVVVSPAQRQAIS
                     DRLAHSSAVDASQTQENPVVPQLYPILVNGRDIDTILLGVTSKSKLDRQDIVGDLTSL
                     QGFDLAGGKQNIPPLTISNGRNLRVTDADTNAVILNAELEQAPVNLRAGDAIVVQSED
                     GKVTRILKVVGFYDSSPTNNINFANMLADNKIVEQLGGSLTLEVFSLKVDPDKLPNLR
                     QSLLRAVPSAIILSVVDIDTFVNQVLNNVIIMLTTLASLAMVAGLIIIANAVALAMLE
                     RRREIGILKSVGHTSRSVLATVLIENGLVGLLGSLVAMLLVAGAITALSQFVFHTTLA
                     IGPGLIALLIGATSLITMAVALLVAWRAVRVRPLDVLRYE"
BASE COUNT         1823 a         2765 c         2886 g         1831 t
ORIGIN      
        1 ggctaccttg ccctcgtggc tgtgttcaac ggtgatgtgc gtgtaacgtg tcatgcctgc
       61 tcctcaatta tcctacctat cgtcgtaggt acgcaatgat caatgttccc ggccaaagcc
      121 ggttttgtca tcctgagcgc agcgaaggat ctcggtgtcc ggcgagtcag atccttcgct
      181 gcgctcagga tgacaaaaaa tgaccgatct tgtttagtaa tgttcatcat cgcgtcccta
      241 cgacgatcat cacatcctga agacgccgaa cggtgtttcc ggcatgggcg cgttgagcga
      301 ggcggcgatg cccagcgcca gcactgtgcg cgtgtcgcgc gggtcgatga tgccatcgtc
      361 ccagagccgc gccgtgctgt agtagggatt gccctcctgc tcgtatttgt ccaggatggg
      421 ctgtttgaac tcggcctctt cctcttcgct catacgcagg ccgcgcgcgg ccagcccatc
      481 cttgcgcaca gtcgccagca cgctcgcggc ctgctcgccg cccatcacgg agatgcgcgc
      541 attaggccac atccacaact ggcgcggcga gtaggcgcgc ccgctcatgc cgtagttgcc
      601 cgcgccgaac gagccgccgg tgatgacggt gaagcgcggc accggcacgt tggcgaccgc
      661 catgaccatc ttggccccgt ctttggtgat accgccgttt tcgtattgtt tgccgaccat
      721 gaagccggtg atgttttgca ggaagataag cggcgttttg cgctgcccgc agagttccat
      781 aaagtgcgtg gccttcagcg cgctttccga aaagaggata ccgttgttgg cgatgatgcc
      841 gaccgggtag cccatcagcc gcgcgaagcc gcacacaatg gtcgtgccgt agagggcttt
      901 gaactcgtgg aagcggctgc catccaccag ccgcgcgatg atctcgcgca cgtcgtagct
      961 tttgcgattg tcgctcggca cgatgccgta tagatcggcg ggatcgtaga gcggcggctc
     1021 aggcgggtga atatcccagg ggatgtactt gcgccggttg aggttggaga cgatactgcg
     1081 gcagatagcc agggcgtgtt cgtcgttcag cgcgtagtgg tcggtcacgc cagaaatgcg
     1141 actgtgtacg tccgcgcctc ccaactcctc ggcgctgacc tcctcgccgg tggcggcctt
     1201 gaccagcggc ggacccgcca ggaagatggt gccgttgccg cgcacgatga ccgtctcgtc
     1261 gctcatggcc gggatatacg cgccgcccgc cgtgcaggag cccatcacgg cggaaatctg
     1321 cggaatgccc tggctcgaca tgcgcgcctc gttataaaag acgcgcccga aatgctcgcg
     1381 gtcggggaag acctccgcct gcaagggcag gaacgcgccg cccgaatcga ccaggtagat
     1441 gcagggcagg tggttctgct cggcgatctc ctgcgcgcgc agatgtttct tgacggttat
     1501 cgggtaatag gtgccgccct tgacggtggc atcgttagcg acaatcacgc actcctgtcc
     1561 ctcgaccacg ccgatgccgg tcacgatgcc cgccgaaggc gcttcattct cgtacatgtc
     1621 ccacgccgcc aacggactga attccaggaa cggcgtttca gggtcgcaca gcagttggat
     1681 gcgctccctc gccagcagct tgttgcgctt gcggtgcttt gcaatggcat tctcgccgcc
     1741 gccctgctgc gcctgtcgca cgcgctcgtt caactgcgcg agcagcccct ggaagtagcg
     1801 ctcgttctcc tgaaattcgg ggctggtgcg ctccacgcga ctttcaatga tgcccatgtg
     1861 cttgcctatt cctctgtttt gtatggcgcg ttttcttctt tgacgcgctc cgcaagaaaa
     1921 atcttcatca aagattggta gggaacgtcc cttttgtttg ccagagactt gaattccgcg
     1981 atcaatgact ccggcaagcg gatagaaacg gtcctggtcg atggcttgag attcggaaag
     2041 acagggtctc gtacagcttt actccaatcc acgtaatcga ttgaataatg atacaataca
     2101 accaactgag cgtcaattct gtcttttccc tcacagttcg cggtcccctg tcaccccgga
     2161 acggctctat gccacgatag tacacctcaa tttcgatttc cacttgattt actactgagc
     2221 ttatggtact ctttgttcgg gacttcaatt attaggaggc gcacagctat gcgtatgtgg
     2281 ataaccacgc tcatgcattt cacaaatttc atcggaccat gtatgagtgc gccctgcgat
     2341 acccgctgat ttcggctgaa tggagatagc ggccacgggc gacactctcg ctcgtggctt
     2401 ttttgtttgt tctgaacggc cacgagcgta atgcgcctcg tggccttctt ctcgtcccct
     2461 tcatgcagga tgcgttggcg aggcattgtt ccctaaccca aatatgtatg gatgaatcgt
     2521 acagatcata cgccttgaaa acgaaaggat gttctcgtga agcttccctt gaaacaatac
     2581 ctcaaactgc tggcgcatta tctgcggcct caaaggacac gggcactttt gatgagtgcg
     2641 ctgctgctgg tcagcatcgg actgcaactg gcgaacccgc aggtgctgcg ctacttcatc
     2701 gatactgcca ccggcggagg gaacccgtcg ctgctgctgc tcgcggcggc gctgttcatc
     2761 ggcatcgctg tcatcaatca agcggtctca atctactcac gctacctgag cgaaaatgtg
     2821 gcctggactg ctacgaacgg gatgcgcgcc gatctgctgg cgcattgcct ttcgctcgat
     2881 atggcttttc ataaagagca tacacccggc gaattgatcg agcgtatcga tggagatgtg
     2941 aatatcctct ccaatttctt ctcggctttc gtcgtctacc tgctgggcaa cgccattttg
     3001 ctggtcggca tcttgctctt tctcttgcaa atcgactggc gtgtcgggct ggccctcgcc
     3061 atcttcgtcc tgctggctct gttcatcttg ataagcattc gcgggcgcat catcccactc
     3121 tggacggcgg accggcagat ggatgccgaa ttttacggct tcctcggaga gcaactggcc
     3181 gggacggagg atatccgcgc gaacggggcg acagcctaca ttatgcgtcg ctttcacctg
     3241 ctcttgaacc ggtggtaccc cattcaccgc aaggccggaa tgggctactt cgcgatgggc
     3301 atgacgacgc tgacgctgtt cgccatcggc aacgcgctgg cattcgccct cggcgcgtac
     3361 ctgtggagca tcaacaaaat ctcgctcggt actgtctaca tcatcttcta ttacaccaac
     3421 ctgttgaccc agccaatcga tcaaattcgc ttccagttgc aagatttgca aaaagcgggc
     3481 gccagcatcc aacgagtcga agcgctgcta cttctccaac cagacatcac cgacgggaaa
     3541 ggtacgccgc tgccggaggg tgcgctttcc gtcgtattcg agcatgtatc cttcggctat
     3601 acgaaagaca cgactgtgtt ggaggatatc accttctcct tgcaggccaa ccgcatactt
     3661 ggtgtactgg gcagaacggg cagcggcaaa acgacgctgg cgcgcctgct gctgcgcatg
     3721 tacgacccgc aaggcgggca gattcaactt ggagacgttc ccattgcaga gccacgcctg
     3781 gcggatctgc gacggcatgt cggcatggtg acgcaagacg tgcaactatt ccatgccagt
     3841 gtgcgcgaca acctgacatt cttcgaccac gccattcccg acgaacgcat cctcgccgtg
     3901 atggacgacc tcggcctgat gccctggtat cgcacactgc cagacggcct ggacagcgag
     3961 cttggcgcga acggcgaggg attgtcggct ggcgaagcgc agttgctggc ctttacgcgc
     4021 gtcttcctgg cgaatccggg gctggtcatt ctcgacgagg catcatcgcg gcttgatcct
     4081 gccaccgaac acctgatcga gcaggcggtg agcaagctgc tgcgcggacg caccggcatc
     4141 gtcatcgcgc accgcctggg aacgatcaaa cgcgccgacg aaattctggt gctggaaaat
     4201 ggacgcattc tcgaatacgg cggcagggcg gcgctggcaa gcgaccacgc ttcacgcttc
     4261 gcttcgctgc tgcaaactgg cctggaggag gtgacaatat gaccactttt caattcctat
     4321 ggcgcatgat acgctaccgt ccctggctct acctgctcaa ctgcatactt tggacgctga
     4381 tccacctgtc gccgctgata ccggggttga tcgcactgga attcttcaac acgctggcgg
     4441 ccagcgccca cctcagcctc gcgctgtggg ggctgatcgc gctgctggtg atgacggccc
     4501 tggcgcgcgt ggcgatattc ctgtgcggcg ggctggcgga tatcctgcac cgcttcacca
     4561 tgagcgcgct gctgcggcgc aacctgctgg aacgcatcct ggagcgtccg ggcgcgcgag
     4621 ccgtgccgta ttcggtgggc gaggccatca gccgttttcg cgatgacgcc gagctggccg
     4681 aggacgccat cagctggtcg ctggatacca tcggcgaggc gtgtttcgcg tcggtggccg
     4741 tctccatcct ggtgatgatc agcgctaaaa tcaccctgct ggtcttcctg ccgctcgtca
     4801 gcgtcatcgc catcgtgcaa gctctcagca accgcctgga acgctaccgc gcagccagcc
     4861 gcaaggccac cggacaggtg acgggcatga tcggcgaaac attcggcatg gcgcaggcca
     4921 tcaaagtcgc gggcgctgaa aggcatgtcg tcaaacactt cgagggcttg aatgagcagc
     4981 gccgcgtggc catgctgaaa gacgctgtgc tgacccagat gctcgactca accttcgaga
     5041 acacggtcgg cctgggaacg ggtctgatct tgatcctggc ggcgcaatcg ctgggcagcg
     5101 ccaacttcag cgtcggcgac ctcgcgctct tcatctacta cctggccttc gtgactgatt
     5161 tctcgcacct cttcggcatg atgctggccc actaccgcca gacaaaggtc tcgttcgagc
     5221 gcatggtcac gctgctgcaa ggcgcgccgc ccgcctcgct ggtcaaatac gagccgctct
     5281 acctgacgag gccggtgccg gaggatggcc acacattgga tggccacaca ccggatgccc
     5341 acaaggggca tccctacata tgggatgatc ccccatccca accccagatt gtcggagtcg
     5401 atatcctggt cgagaccgat ggattgatgt accgctatcc cgatacaggg cgcggcatcg
     5461 aagatgtgag cctgcgtcta aagcgaggtg cgctgacggt ggtgactggg cgcatcgggt
     5521 cggggaagac gacgctcttg cgggcgctgc tgggactgtt gccgaaggat gtcggggaaa
     5581 tcaggtggaa tggcgcgccg gtcgatgatc ccgcctcgtt tttcgtgccg ccgcgcagcg
     5641 cgtacacctc gcagataccg cagttattca gcgagacgtt gcaggagaat atcctgctcg
     5701 gcctgccgga ggcgcgggtc gatctacagg ccgccgttga gatggcggtc atggagcagg
     5761 atattgcgga actggagcag gggctggcga cgatgatcgg tacgaagggc gtcaagctgt
     5821 cgggcgggca ggcgcagcga gccgccgcag cgcgcatgtt cgtgcgcgag cctgagctgc
     5881 tggtgtgcga cgacctctcc agcgcgctcg atgtggagac ggaacgcaca ctgtgggagc
     5941 gcctcttcgc ccgccgcgcc aacgccagca catgcctggt cgtctcgcac cgccgcccgg
     6001 tcttgcagcg cgccgaccac atcatagtcc tcaaagatgg ccgagtcgag gctgagggaa
     6061 cgcttgacga gttgctgcaa acgtgcgaag agatgcggcg cttgtggagc ggggaagtga
     6121 acgcgaatgc caggtaattc attaaaaatg ttaccgacta tcctcgatgt tgtcatgctg
     6181 agcgcagcga agcatctctc acccaacaac gagacccttc gctgcgctca gggtgacaat
     6241 cgatgaccga aactggatgt gaaaaatcac taagaatcag cgatcttgca gttcccaggc
     6301 atgatcgagg gcgtgccagg ccgacctgcg aacagcaaag cgcggcatcc acgcaaagcc
     6361 atacctgcgg agaggcgcga ccgtctgccc gcacggcact gcacgtagct gcgccagaat
     6421 ctgttctcgc accgacgttt cctgttcggc cacgcgagcc gggtccggat gccggaaagc
     6481 cgggccaaag gctgagaggt gcatcaaatc tgcctccaac atgtgcaggc gcatggcatc
     6541 cggcgaacgc ccgcgctcag gtttcacccc atgagcctct ggtgggatgc agatgaaaat
     6601 atcatcgaac gtggtccaac tggcagtgag caggccggcg agccgttcga tatcccctac
     6661 ttcgagcggc tcctggtcgg atgcgagtag gacagatgga gcgccgaagt cggttgtagc
     6721 cgtcccaggc acacgctcaa ccacctcgaa atcttcgatg gaggaagggg gaatgaaggc
     6781 aagtctggca gccccggcta ccagtgcata gcgttgggcg gtggtgacga gattgcccag
     6841 cgccactgtt tcgtctttgc ctgaccggca ccagccgatc cattcaagcg cctgggcaaa
     6901 gacgcggcgc ttcgggccga tttctagagc aattgggaat ttttgcatag tgacctcatt
     6961 ctattcatac cgcagcacat ccagcggcct caccctgacc gcgcgccacg ccaccagcag
     7021 agcgacggcc atcgtgatga gcgaggttgc gccgatgagc agggcgatga ggccggggcc
     7081 gatggccagg gtggtgtgga agacgaactg gctgagggcg gtgatggcgc cggcgacgag
     7141 cagcatggcg accagggagc cgagcaggcc gaccaggccg ttttcgatca ggacggtggc
     7201 cagcacggag cggctggtgt ggccgaccga tttgaggatg ccgatctcgc ggcggcgttc
     7261 cagcatggcc agcgcgacgg cgttggcaat gatgatgaga ccggcaacca tggccagcga
     7321 tgccagcgta gtgagcatga tgatgacgtt gttgagaacc tggttgacga acgtatcgat
     7381 atccaccacg ctgagaatga tggccgacgg cacggcgcgc aataggctct ggcgcaggtt
     7441 gggcagcttg tcgggatcga ctttgagcga gaagacttcg agtgtgagcg agccgcccag
     7501 ttgctcaacg atcttgttat cggccagcat attggcgaag ttgatattat tggtcggcga
     7561 ggagtcatag aaaccgacga ctttcaggat gcgcgtgacc ttgccgtctt cgctctgcac
     7621 cacaatagca tcaccggcgc gcaagttgac aggtgcttgc tccagttcgg cgttgagaat
     7681 caccgcgttt gtatcagcat cggtaactcg caggttgcgc ccgttgctga tcgtgagcgg
     7741 gggaatgttc tgtttgccgc ccgccaggtc aaagccctgc aaggaggtga gatcgccgac
     7801 gatatcctgc ctgtcgagct tgctcttact ggtgacgccg agcaggatgg tgtcgatgtc
     7861 gcggccattc accaggatgg ggtagagctg cggcacgacg ggattctcct gggtctggct
     7921 ggcatcgacg gcgctggaat gcgccaggcg gtcgctgatg gcctggcgct gggccgggga
     7981 gaccaccacg aacaggttgt gggtaaaaag cgtactgagt tcgctgttga tggcgtcctt
     8041 gatgccctgg cccagcagca ggatcaggcc gatggcgaag acgccgacga agagggcggt
     8101 cagcgttgtg gtggtgcgca ggcgctggcg gccaaggttg cgataggcca gcatcacggc
     8161 ggttttccag gagcgcgggg cgaaggcgac caggccgttg acgatggtcg ccagcaggta
     8221 caccagcgag ccgccgacca ggataatgcc gacgccggcc agcacaacca gcgcatagat
     8281 gccgaccagg ctgttgccga gccgcgtcgc cagcatattc gcgccctcgc cgagcagcgc
     8341 caggccacca aagatcagcg ccgccagcag caggatgccg agcgccagca ggatcgaaag
     8401 caggatgcgc gggcgcggct tttcgtagac aggcaggcgc gagatggcga caaccagcag
     8461 gccgaaaccc agcgcgagcg agaagatcac ccccgcgccg ccgtaaacgg cgatgatggc
     8521 ggtcggcacg tcgcccagga taccagaggc cagcgccacg aagagcagcg aaagcagcag
     8581 cagcagccca agcgtgagca ggcgcgagga gaagcggcgc tgctcgatga cctcgcgcaa
     8641 gacagcgagc gggcgcacct ggccggcctg cacgatgggc agcaggccga agatcaaggc
     8701 ggttgacagg ccgatcaaca ggccggagac gagcgtgagc ggatcgacga tgatgggcag
     8761 gtgaatgaag aaggcttgct cgaccacgcc gcgcacgagg tagctgatgc ccagcccggc
     8821 gagcgagcct gccacgccgc ccgcgatgcc gagcagggcg gtttccaggc cgaagagggc
     8881 gtaaaggtcg cgctggcgat agccggtggt cttgagcata gcgatctcca cctgcctgcg
     8941 gcgcagcaag acctgtacgg tgttgatgat gccgatgccg ccgatgaaga gcgccagcag
     9001 gccgacgatg cgcagaaaga gacggatctg gtcaacctgg gcctggcgtt gttgcagcag
     9061 atcgttggcc gtgatgacac gcgcggaggg gtattgctgg ctcaactgcg ccttgacagc
     9121 gttgacataa gaggcaggca ttgtcatata gacgacgccg tactgcgcgg gttcttttgc
     9181 cccgccggga ccgggcgtgt tgtccagcgt ggactgagca atgatgattt gcgggccgcg
     9241 aaaaacgcca tcctcgcgaa actccgccgc gacggtgatg gagacaaagc ggccatcaag
     9301 ggttt
//
LOCUS       DASZAK010000730         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_124401, whole genome shotgun sequence.
ACCESSION   DASZAK010000730 DASZAK010000000
VERSION     DASZAK010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124401"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..2481
                     /locus_tag="VGT44_12860"
     CDS             43..2481
                     /locus_tag="VGT44_12860"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2581737.1"
                     /translation="MSDLLEGVQLSDFLLMQCINRGGVADVYRGRHVGLAGGSNYDVA
                     VKVFRPGYAQRQSFHDYFMAEADKIGQFEHPNILPFLEFGEGEGLLYSVTPFIESGTL
                     GDLLNRVGGKFPAMQALPIMQQLCSALQYAHERDVIHGNIKLSNVFVGTDGRMLLADF
                     GIVRGFDDSQQSLTRVGWGSAEYAAPEQSLGVLRRASDIYALGALLFRMLTGQPPFTG
                     QTPVEVLLKHVRLQTPSARSIEPSISDTVDAVLQRAMQKRSDDRFASASEFFDALQMA
                     VTVAPVASPVARAVPAITRQLIHGQTATGGTQDNQVHSASLFQQMSPVGEPAYKEDIG
                     ASSLHRGSPAEADQQQFLLDKDDAGTHIFWSVDPLEWSPIGKEQVGSVPLTAEDYLQS
                     MPVAPHKPLFDSAAPISPPRAIEPVGVEAPVLPAVMPAQPHTTIPLSEPDQLVKETKD
                     KQAKRAGRKKMLPVLVVILLLIGLLGALLSSFLYPSPSGNNSTTRSGATSTPAPGATS
                     TPAGATNPTGASVSPVAGSPTATVATTTTPLPSPSPTSIPIPANPAVPQFTCASGTLA
                     MDGSASFIPAIYQLTTDYNNQCSSSVNFSFNTDDSQTGLSNLVNGSVGLAYSDLPSSG
                     RPGLVDYQVAALIFAVVVNSDTQVTNLTTVQLQDIYTGRYTNWSQVGGSNEPIVILTQ
                     PPGSTLRTIFETYVLGGTAQTVPGLSSAASITNLSGGITYMPLADAPATGAQVITING
                     TSATASTVKSGAYSFWSIEHLYTDHAAQGVALSFIAFCLHSLSANDFATSGSVPYAAM
                     SDAALRTHLPSPTI"
     gene            2512..>3106
                     /locus_tag="VGT44_12865"
     CDS             2512..>3106
                     /locus_tag="VGT44_12865"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2581738.1"
                     /translation="MADLAGVMVGNYFLLECLGHEGIVETYRARPTTRGGCDVLMRIF
                     RPSFPDLTGFQDHFMAEVEKVWRCRHDHILPLIEYGAGDGLLYCVSELVEAQTLERYL
                     EQHERTGATLPVPLVVRWTTQLCEALAYAHERGIAHGNLQPSGILLRDDDTVLLTNFS
                     MRRVAQDTDPAVAQVEEGNAAYIAPEQAVGMLSPASDI"
BASE COUNT          593 a          962 c          901 g          650 t
ORIGIN      
        1 tcgttgaact gaaatcatat cacgactatt gtggggtgtc gcatgtctga tcttcttgaa
       61 ggtgtacaac tcagtgattt tctcttaatg cagtgtatca atcgtggagg cgtggcggat
      121 gtctatcgcg ggcggcatgt gggcctggcg gggggatcga attatgatgt tgcggtcaag
      181 gtcttccggc caggatatgc tcaacggcaa tcgtttcatg attactttat ggctgaagca
      241 gataaaatcg gccagtttga gcatccgaat atcctgcctt ttctggaatt tggcgagggc
      301 gagggcctgc tatattcggt cacacccttc atcgaaagcg gaacgctggg cgacctgctg
      361 aaccgcgtgg gtggcaagtt tccggcgatg caggcgctgc cgattatgca gcagttgtgc
      421 agcgcgctgc aatacgcgca cgagcgcgat gtcattcacg gcaatatcaa gctctctaac
      481 gtcttcgtag gcaccgatgg gcgcatgctg ctggccgact tcggcattgt gcgcggcttt
      541 gacgatagcc agcaatctct cacgcgcgtc ggctggggtt cggcggaata tgccgcgccg
      601 gagcaatcgc tgggcgtgct gcgccgcgcc agcgatatct acgccctggg cgcgctgctc
      661 ttccgcatgc tcaccggcca gccgcccttc accggccaga caccggtcga agtgctgctc
      721 aagcacgtgc gtttgcagac cccctcggcc cgctcgattg agccgtctat ctctgatacc
      781 gtcgatgctg tgctgcagcg cgccatgcag aaacgctccg acgaccgatt cgcctcggca
      841 agtgagtttt tcgacgcgct gcaaatggct gtgacggtcg cgccggttgc ctcaccggtc
      901 gcaagggcag tcccggccat tacgcgccag ttgatacacg gtcagaccgc aacgggcggt
      961 acgcaggaca accaggttca ctccgcgtcg ctgttccagc agatgtcacc ggtcggcgaa
     1021 cccgcctata aagaggacat cggcgcgagc agtttacatc gtggaagccc tgctgaagcc
     1081 gatcagcagc aatttttgct ggataaagac gatgccggga cgcatatctt ctggtcggtc
     1141 gatccgctcg aatggtctcc aatcggcaaa gagcaggtag gatcggtacc gttgaccgcc
     1201 gaggactacc tgcagagtat gccggtggcg ccgcacaaac cgctgttcga ttctgccgcg
     1261 ccgatctcgc ctcccagggc tatcgagccg gtcggcgtag aagcgcctgt cctgcccgca
     1321 gtcatgccgg cgcaacccca cacaaccatt cctttgagcg aacctgacca gcttgtcaaa
     1381 gagacgaaag acaaacaggc aaagcgcgcc gggcgcaaaa aaatgctgcc ggtgctggtc
     1441 gtcatcctgc tgctaatcgg cttgctgggt gcgctgctct cctcgtttct ctatcctagc
     1501 ccatccggca ataactcgac cactcgctct ggcgcgacct cgaccccggc gcccggcgcg
     1561 acctcgactc cggcaggcgc gacaaatcct acgggcgcga gcgtctcgcc tgtggcaggt
     1621 tcgccgaccg cgaccgtggc gacgacgacc acgcctttgc ccagcccaag cccgacctcc
     1681 attcccatcc cggccaaccc ggcggtgccg cagttcacct gcgcctccgg tacgctggcc
     1741 atggatggct cggccagctt catccccgcc atctatcaat taacgaccga ttacaacaac
     1801 cagtgttcca gcagcgtcaa tttcagtttc aacaccgatg atagccagac cggtttgagc
     1861 aacctggtga acggcagcgt tggcctggcc tattccgatc tgccctcgtc aggtcgccct
     1921 ggactggtgg attaccaggt ggccgcgctc atcttcgccg tcgtcgtcaa tagcgatacc
     1981 caggtcacca acctgacgac cgtgcagttg caggacattt atactggccg ttacaccaac
     2041 tggtcgcagg tgggcgggtc gaatgaaccc atcgtcattc tcacgcagcc gccagggtca
     2101 acgctgcgca cgatctttga aacctacgtg ctaggcggca cggcgcagac ggtacccggc
     2161 ctctcctcgg cggcgagcat cacgaatctt tccggcggca tcacctatat gccactggcg
     2221 gatgccccgg ctaccggcgc gcaggtcatt accatcaacg gcacaagcgc aacggcatcg
     2281 acggtcaaga gcggcgcgta ctctttttgg agcatcgagc atctctacac cgatcacgcc
     2341 gcccagggag tagctctgtc tttcatcgct ttttgtctcc actcactcag cgcgaatgac
     2401 ttcgccacta gcgggtctgt cccatacgcg gctatgtcgg atgccgcgct caggacgcat
     2461 ttaccgagtc cgaccattta atggattttg atagggaggg aaagacgcac gatggctgat
     2521 cttgcagggg tcatggtagg gaactacttt ttgttggaat gcctcggtca cgagggcatt
     2581 gtcgagacgt atcgcgcccg cccgacgacg cgcggcggat gcgatgtcct catgcgcatc
     2641 tttcgccctt cgtttcctga tctgaccggt ttccaggatc atttcatggc cgaagtcgag
     2701 aaagtctggc gctgtcgcca tgaccatatc ctgcccctga tcgaatacgg cgcgggcgat
     2761 gggctgctgt actgtgtttc ggagctggtg gaggctcaaa cgcttgagcg ctacctggag
     2821 cagcatgaac gcacgggtgc gaccctgcct gtgccgctcg ttgtgcgctg gacaacgcag
     2881 ttgtgcgagg cattggccta cgcccatgaa cgcggcatcg cgcacggcaa tctccagcca
     2941 tcgggtatcc tgctgcgcga tgatgatacc gtgctgttga ccaacttcag catgaggcgc
     3001 gtcgcccagg acaccgaccc ggctgtggcc caggttgaag aaggcaatgc agcgtatatc
     3061 gcgccggagc aggccgttgg catgttgtcg cccgccagcg atattt
//
LOCUS       DASZAK010000731         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_623887, whole genome shotgun sequence.
ACCESSION   DASZAK010000731 DASZAK010000000
VERSION     DASZAK010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623887"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VGT44_12870"
     CDS             complement(<1..579)
                     /locus_tag="VGT44_12870"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2581739.1"
                     /translation="MQWFKHLWNQRYPLMISVLVCSCIGLAACGGPPNNPDVCSIVPP
                     SGYPGLVMANGNVYLQGGYSTSIYALRMSDGALQWKYHTGALIGIDSGIVYTQGLGDI
                     LYAVRATDGKQLWHYDMGPDVSTVDGVVDGLVYLSSSNSLTMYTLRGSDGARLWRHKI
                     DVDHFPTRFAASNGIVYVASDYSRILALRESDG"
     gene            883..1296
                     /locus_tag="VGT44_12875"
     CDS             883..1296
                     /locus_tag="VGT44_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2581740.1"
                     /translation="MAENMTAVPNMKLELVPVPVSDVDRARAFYVEKAGFKLEVDVQP
                     TDTMRVVQLTPPGSACSIVFGTGMGEITDMQPGSVKGLHLVVADIHEAREALVKRGLE
                     VAEAWDIQGVKYAGFSDPDGNMWLLQEFPAHLRRA"
     gene            1300..>2163
                     /locus_tag="VGT44_12880"
     CDS             1300..>2163
                     /locus_tag="VGT44_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV2581741.1"
                     /translation="MRALIYANGSLHLDRDFPHPSPVPGEALIRVTMAGICNTDIEIT
                     RGYLDFQGIPGHEFVGTVEELYGAIAQKSHKRLIGKRVVGEINAACHRTDCYYCSRAM
                     PTHCPNRTTLGIVRRNGAFADYLMLPVENLHVVPDNVSDEEAVFVEPLAANFEILERA
                     HVRPTDLVIVLGDGKMGQLAARVMALSGCDVRMVGKHREKMALVEPLGITTHLLAEAE
                     HILADMGRRVDMVIECTGSASGLELALRLARPRGTVILKSTVADKSTLALAPIVIDEI
                     RVQGSRCGPFQP"
BASE COUNT          506 a          635 c          567 g          455 t
ORIGIN      
        1 tccatcgctt tctcgcagcg ccagaatacg agaataatcc gaggcgacat acacaatgcc
       61 attgctggct gcgaagcgcg tgggaaaatg gtcaacgtcg atcttatgcc gccagaggcg
      121 agcgccatcg ctgcccctca aggtatacat agtcaatgaa ttactcgaac tcaggtagac
      181 aagcccatcc acaaccccat caaccgttga aacatcggga cccatgtcat aatgccagag
      241 ctgtttgcca tctgtggcac gcaccgcgta gagaatatca cctaacccct gtgtatagac
      301 aataccgcta tctatgccga tcaaggcgcc agtatgatat ttccattgca aagcgccatc
      361 gctcatacgc aacgcgtaga ttgaagtgga atagcctccc tggagataaa cgtttccatt
      421 tgccatcaca agccctggat aaccagatgg aggaacgatt gaacagacat cggggttatt
      481 gggagggcca ccacaggctg caagaccaat gcaggaacac accagcacac ttatcataag
      541 agggtaacgt tgattccaaa gatgcttgaa ccactgcatg ccttttctct ttcttatcac
      601 accccattcc gcgatgcgta gattcttaac aagagatgct actcgttgaa cgctctggat
      661 gagatgtttt agagtgtttt attcgcagcc tggcttgaca tgtaatcgat tctccaatag
      721 aatacatgcg tccatcaatc cattagaatt tgtccttcct ggcgtggtgt tgctgcccct
      781 tgtaatgtgg gcttatagtg cgattctcaa acgcatctct gcaagccgcc agcctgtggc
      841 cgtttaggaa gtacgactac tctagaacaa ggagcaacca cgatggcaga gaacatgacc
      901 gcagtaccca atatgaaact tgagctggtg ccggtcccgg tgtctgatgt tgaccgcgcc
      961 agggctttct acgttgaaaa ggccggtttt aagttggagg ttgatgtgca gcctaccgac
     1021 acgatgagag ttgtccagct tacgcctccc ggctcagcct gttcgatcgt cttcggcacc
     1081 ggcatgggag aaattactga catgcagcct ggatcggtca aaggtctaca cctggttgtc
     1141 gcggatatac acgaggctcg cgaagcgctc gtgaaacggg ggcttgaagt tgccgaagcg
     1201 tgggacatac agggagtaaa gtatgccggt ttcagcgatc ccgatggcaa catgtggctc
     1261 ctgcaagaat tcccggctca ccttagaaga gcatagcaca tgcgcgccct catctacgcc
     1321 aacggcagtc tccatcttga ccgcgacttc ccccaccctt cgcccgtacc cggcgaagcc
     1381 ctcatccgcg tcacgatggc cggtatctgc aacaccgaca tcgaaatcac gcgcggctac
     1441 ctggatttcc agggcattcc tggtcacgag ttcgtcggca ccgtagagga actctacggc
     1501 gcgatagccc agaagagcca taagcgcttg atcggcaagc gtgtagtcgg tgaaatcaac
     1561 gcggcctgcc atcgcaccga ctgctactac tgctcgcgcg caatgccgac gcattgcccg
     1621 aaccgcacca cgctcggtat cgtgcggcgc aatggcgcgt tcgccgatta cctcatgctc
     1681 cccgtcgaga acctgcacgt cgtgcccgac aacgtcagcg atgaagaggc cgtctttgtc
     1741 gagccgctgg ccgccaactt tgagatactg gaacgggcgc acgtcaggcc gaccgacctg
     1801 gtgatcgtgc tgggcgatgg caagatgggc cagcttgccg cgcgggtcat ggcgctatca
     1861 ggctgcgacg tgcgtatggt aggcaagcat cgcgagaaaa tggcgctcgt cgagccgctc
     1921 ggcatcacca ctcatcttct cgctgaagca gaacatatcc tggccgacat gggacgccgc
     1981 gtagatatgg taatagaatg taccggctcg gcgagtggcc tggaactggc cttgcgcctc
     2041 gccagaccgc gcggcacagt catcctcaaa tccaccgtcg ccgacaaaag cacactcgcc
     2101 ctcgccccca tcgtcatcga tgagattcgc gtgcagggat cgcgctgcgg ccccttccaa
     2161 cca
//
LOCUS       DASZAK010000732         1912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_231506, whole genome shotgun sequence.
ACCESSION   DASZAK010000732 DASZAK010000000
VERSION     DASZAK010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231506"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1077
                     /locus_tag="VGT44_12885"
     CDS             295..1077
                     /locus_tag="VGT44_12885"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV2581742.1"
                     /translation="MRPLIGIPCHADFRDGSRRPVYCNNRTYIHAVERAGGVPVLIPL
                     YEDLSLMDTLLPRLDGLLLSGGADVEPTRYGEARNPLTDEPDKMLDEIELNLASWAVQ
                     EDIPTLGVCRGMQVLNVALGGTLYQDIHDCCPGSLPHSRRELPRDFLAHSVEALPGSR
                     MEAVLGTRTIMVNSLHHQAVHEPGKGVVISGRAQDGIAELLEVPANRFALAVQFHPEE
                     IYMKEAPSARLFKAFVKACSNVTGEEADELVVVGAVERSLAM"
     gene            complement(1097..1633)
                     /locus_tag="VGT44_12890"
     CDS             complement(1097..1633)
                     /locus_tag="VGT44_12890"
                     /inference="COORDINATES: protein motif:HMM:NF018749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HEV2581743.1"
                     /translation="MQWYNEPPRWSVEGDIVMIQSGAKTDFWRLTHYGFTRDNGHFYY
                     QPQAGDFLAEVKFSGDYAATYDQAGLMVRVDESTWVKCGIEYIDGVQQVSAVVTRDYS
                     DWSVVPLAHNPAALWLRLKRMGSALEIQYALDGVSYQLLRLAYFSPAETMDVGLLCAS
                     PEGEGFPARFEDFTIQSL"
BASE COUNT          426 a          524 c          539 g          423 t
ORIGIN      
        1 ttggctagct caggcatggc gataccccac atacttgaca gcacggcgta tattacaaga
       61 agaaacagtt taacgactgc cacatcacta aggaacaaaa cgtgagagaa cgtagagcgc
      121 ctgttgtacc ggctagcggg attcttctta cacagctcca gttcgtgctg gagcttcttg
      181 tgattgagcc tggacagagg gcggtgcgtt tctaacggta aatgaaatga ctttttcagt
      241 ggaacgcatt gacggcgttc cactttttta ttgaatgttg aaggagaatt tgtaatgcgc
      301 ccattgatcg gcattccgtg ccacgcggat tttcgcgatg gttcacgacg gccagtgtat
      361 tgtaacaatc gcacctatat tcacgcggtg gaacgggctg gcggtgtacc tgtcctgatc
      421 ccattatatg aagacctctc gctgatggat acgctgttac ctcgcctgga tgggctgctg
      481 ctctcaggcg gcgccgatgt cgagccaacg cgctacggcg aagcgcgaaa tcccttgacg
      541 gatgagccgg ataagatgct ggatgaaatc gaactgaacc tggcgagctg ggctgtgcag
      601 gaggatattc ccacgctggg cgtctgtcgc gggatgcaag tgttgaacgt ggcgctgggc
      661 ggcacgctct accaggatat tcacgattgc tgccccggca gtctgccgca ttcgcgccgc
      721 gagctgcccc gcgacttcct cgcgcacagc gtcgaggcgc tgccgggcag tcgcatggag
      781 gccgtgctgg gaacacgcac gatcatggtc aacagcctgc accatcaggc cgtacacgaa
      841 ccgggcaagg gggttgtgat cagcgggcgc gcccaggacg gcatcgcgga actgctcgaa
      901 gtacccgcca accgcttcgc gctggccgtc cagttccacc cggaagaaat ctatatgaag
      961 gaagcgccct ctgcccgtct cttcaaagcc ttcgtcaagg cgtgcagcaa cgtgacaggc
     1021 gaggaggccg acgagctggt tgtggtaggc gctgtggaac gctcgttggc gatgtagtca
     1081 gcgtcaacgt gcgcatctat aggctctgga tcgtaaagtc ttcaaacctg gccggaaatc
     1141 cctctccttc gggtgaggcg cacaacaggc cgacatccat cgtttcggct ggcgagaaat
     1201 acgccagccg gagcaactgg tatgatacgc cgtcgagcgc atactgaatc tctagcgctg
     1261 atcccatgcg cttcaaacgc agccagagcg cggcaggatt gtgcgcgaga ggaaccaccg
     1321 accagtccga atagtcccgc gtcacgacgg cgctgacttg ctgtacgcca tcgatatact
     1381 cgatgccgca tttgacccag gtagactcgt ccacgcgcac catcagcccg gcctggtcat
     1441 aggtggcagc atagtctccg ctgaatttga cctcggccag gaaatcgcct gcctggggtt
     1501 gataatagaa atgcccatta tccctggtga aaccataatg agtgagtctc cagaaatcgg
     1561 ttttggctcc tgattgtatc atcacgatat cgccttcaac tgaccacctg ggaggttcgt
     1621 tgtaccattg cattacgtcg ttcctttcct tgtctggcta cttccacatt atataccgtg
     1681 atacgtcctt gtcctcagtt gagtagagtc acccggattc gatgacgaac agtaaagcgg
     1741 gtatacgcct ccgtaggtgc cgattgatcg cgcacatcgc cgattatgaa cattaacaaa
     1801 ttaagttgga tatggtagac tatcggaaac gaccaatatc acaggaggtt tccgatggca
     1861 ttgcctttct ccctcgcgct gagcgaggag caagaacacg aactggtgca tg
//
LOCUS       DASZAK010000733         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_374877, whole genome shotgun sequence.
ACCESSION   DASZAK010000733 DASZAK010000000
VERSION     DASZAK010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374877"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VGT44_12895"
     CDS             complement(<1..359)
                     /locus_tag="VGT44_12895"
                     /inference="COORDINATES: protein motif:HMM:NF015592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581744.1"
                     /translation="MNLWQISEQTFDPAPKKLHSQESVYTIGNGYFCTRGTFEEGYPQ
                     ANPATLLYGVFDEIPIAKEELANAPDWTPIQLFVNGERFRLDKGHLLDYQRTLDMEHG
                     VLHRTVRWESTTGARLL"
     gene            complement(428..1072)
                     /gene="pgmB"
                     /locus_tag="VGT44_12900"
     CDS             complement(428..1072)
                     /gene="pgmB"
                     /locus_tag="VGT44_12900"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEV2581745.1"
                     /translation="MNKPKAIIFDLDGVLTDTSEYHYQAWKHLADDEGIPFTHEENDA
                     HLRGVGRRDSLLYLLKGRTVSEAQMQEMVDRKNRYYTELIKNMSPKEVVPGGVDLLKE
                     IRQAGIKTAIASASRNTMTVLQRLDLVSYFDGIADGNSVVNGKPAPDVFVFAAGLVNT
                     PTPDCLGVEDATAGIEAIKAAGMTALGIGPKERFPTADKVMASLENKTLQDLLA"
     gene            complement(1131..>2498)
                     /locus_tag="VGT44_12905"
     CDS             complement(1131..>2498)
                     /locus_tag="VGT44_12905"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEV2581746.1"
                     /translation="IVYADDALIEQITQDNALHQVANVATLPGIVGHSLAMPDIHWGY
                     GFPVGGVAATHADYGVVSPGGIGFDINCGVRLLATDLLHNQIRGKVDKLADELFNNLP
                     SGVGGAGMHHLSMDELRAVMVRGSAWAIEEGYGFAEDLEVTEEHGCLAGADPDAVSNT
                     AIQRGMKQLGSLGSGNHFCEVQTIDHIYDAEAAQALGIGQVGQIVVTIHCGSRGFGHQ
                     IAEDYIRLAESKQQDFGFHLVDRQLACLPLQSNLGKSYLAAMACGANFAWANRQLLMH
                     GVRQAFATVFGRQARPKNMPLVYDVCHNIAKLEQYEIDGQLQRVCVHRKGATRAFPAN
                     HPAVPEKYRAVGQPVLIPGDMGRYSFVLVGAQGSMEQTFGTTCHGAGRRQSRTAAKKS
                     ITSKELLEQLAAKGVTVRVHSKNLLPEEAPQAYKDAQQVVNVVHNAGLAKLVVRLKPV
                     IVVKG"
BASE COUNT          527 a          694 c          683 g          594 t
ORIGIN      
        1 atgagaaggc gtgcacccgt tgtgctttcc cagcggaccg tgcggtgcag cacgccatgt
       61 tccatgtcca gggtgcgttg atagtcaaga agatgaccct tgtcgagacg gaaccgttct
      121 ccattcacaa acagttgaat aggtgtccaa tcgggagcgt ttgccagttc ttctttcgcg
      181 atgggaattt cgtcaaaaac accatagagc aaggtcgcgg gattcgcctg agggtaaccc
      241 tcttcaaatg tgccgcgtgt gcagaagtag ccgttgccaa tagtatagac gctctcttgg
      301 ctgtggagct tcttcggtgc agggtcaaag gtctgttcgc tgatctgcca aaggttcaca
      361 aaaaccccct tcttcaaaaa tgcactggta tgttttgaat gaactctgac gttcattgcg
      421 tttattgtta cgcgagcaag tcctggagtg ttttattttc aaggctggcc atgaccttgt
      481 ctgccgtggg gaaacgctct tttgggccga tcccaagagc cgtcatccct gctgctttta
      541 tcgcctcgat accagcagtg gcgtcctcaa cccccaggca gtccggcgtt ggcgtgttga
      601 cgagaccggc cgcaaagacg aagacatccg gtgccggctt cccattaaca acactattgc
      661 catcagcgat gccatcgaaa taggaaacga ggtccaaccg ctgtaataca gtcatcgtgt
      721 ttctgctggc agaggcaatg gccgttttga tgcctgcctg tcgtatctct ttcaagaggt
      781 ctacaccgcc cggtacgacc tctttgggcg acatattctt gatcagctct gtgtaatatc
      841 gatttttgcg atccaccatt tcctgcatct gcgcttcaga caccgtgcgt cccttgagca
      901 ggtacagaag agagtcgcgt ctaccaacac cgcgcaggtg cgcgtcgttc tcctcgtgtg
      961 tgaaaggaat accctcatca tcagccaggt gtttccaggc ttgataatgg tactctgaag
     1021 tgtcggtgag gacaccatcc aggtcgaaga tgattgcctt tggtttgttc ataagaatcc
     1081 tccaaaattg agaatcgata gtatctcgac tttcgcagaa cgtacagagt ttaccctttg
     1141 accacaatga ccggcttcag ccggactacc agtttggcca gcccggcgtt atgcaccaca
     1201 ttcaccacct gctgcgcatc cttgtaggcc tggggagcct cttcgggcag caggttcttg
     1261 ctgtgaacac gcaccgttac acctttggca gcgagttgct cgagcagctc tttgctggtg
     1321 atcgactttt tggcggcggt acggctctgg cgacgcccgg cgccgtgaca ggtggtgccg
     1381 aatgtctgct ccattgagcc ttgcgcaccc accaggacga aggagtagcg acccatgtcg
     1441 cctggtatga gcacgggctg tcccacggca cgatacttct ctggcacggc aggatgattc
     1501 gcgggaaatg ctcgcgttgc tcccttgcgg tggacacaga cgcgctgcag ctggccgtcg
     1561 atttcgtact gctccaattt ggcgatgttg tggcagacat cgtagacgag aggcatattc
     1621 ttgggacgtg cctgtcgtcc aaagaccgtt gcgaatgctt gacgcacgcc atgcatcaac
     1681 agttgacggt tggcccaggc gaagtttgca ccgcaggcca tggctgcaag gtacgacttc
     1741 cccaggttcg actgcaatgg taaacaggcc aactgccgat ctaccaggtg gaagccgaaa
     1801 tcctgctgtt ttgactcggc caacctgata taatcctcgg cgatttgatg accgaaaccg
     1861 cgtgagccac agtgaatcgt cacgacgatc tgaccgacct ggccgatacc cagggcctgg
     1921 gcagcttcgg catcgtagat gtgatcaatg gtttgcactt cacagaagtg attacccgat
     1981 cccaggctgc cgagttgctt cataccacgc tgtatggcag tattgctgac ggcgtcggga
     2041 tcagcgccag caaggcagcc atgctcttcg gtcacctcga gatcctcggc aaagccatag
     2101 ccctcttcga tagcccaggc agacccacgt accatgacag cacgcaattc gtccatgctc
     2161 aggtggtgca tacctgcacc acctacgccc gatggcaggt tgttgaagag ttcgtcggcg
     2221 agcttatcga ccttgccgcg aatctggtta tgtaacaggt cggtggcgag taagcgcacg
     2281 ccacagttga tgtcgaagcc gatgccaccg ggggatacga ctccgtagtc agcatgggtt
     2341 gctgctacac cgccgacagg aaagccgtag ccccagtgaa tgtcgggcat ggccagcgaa
     2401 tgcccgacga tgcctggcaa tgttgctacg ttggcgactt gatgcagcgc gttatcctgc
     2461 gtaatctgtt ctatcaatgc gtcgtcggcg tagacgat
//
LOCUS       DASZAK010000734         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_90374, whole genome shotgun sequence.
ACCESSION   DASZAK010000734 DASZAK010000000
VERSION     DASZAK010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90374"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VGT44_12910"
     CDS             complement(<1..465)
                     /locus_tag="VGT44_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2581747.1"
                     /translation="MSSTAPLPGITQHIFQTDRLKVAYLEAGTGDVPVVLIHGNCSSS
                     AFFQDFMLALVATGRYRIFAPDMRGYGDSETLPVDATRGVRDFSDDLDAFARATNLTS
                     FHLLGWSLGGNVVMQYAIDYPGMLRSLMLESPGSPFGFGGTKDATGSPTWDDF"
     gene            complement(591..1268)
                     /locus_tag="VGT44_12915"
     CDS             complement(591..1268)
                     /locus_tag="VGT44_12915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581748.1"
                     /translation="MQQARLGKPALQNGLLFGLTLGVAEIVLSYLLGTPGLLISVLLY
                     LFMVGFAGYRASTRTGKVSTGLVAGLFAGLFSSIIATIPLVVYYLSNIDAFRVQLQQQ
                     MTASNLNQGITITNSLVIASVILFLVVVVAGATLLGLGIGSIGGAIGKGQAGSPPMSQ
                     YPSYTRPSYPPQGIATPPPQGYTPPPPPQTYQEFVPSGDYTSPEAYMLPQEKKPPTPP
                     GPYGSQD"
     gene            complement(1356..2927)
                     /gene="zwf"
                     /locus_tag="VGT44_12920"
     CDS             complement(1356..2927)
                     /gene="zwf"
                     /locus_tag="VGT44_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV2581749.1"
                     /translation="MTQQTQANIPLKQHSIWQDGQTPEPCAVVIFGATGDLTQRKLLP
                     TLAHLAHDHPLPQGFSVVAFARRPMTDEQWRAMALDSINQFMPDDDKLDEKAQKAFAQ
                     KLFYCQADFNDREGYAKLADLLEKLDAEEGTQGNRLYYLATPPTLDSEIIHQLGGAGL
                     ARPRQSNDNNGEESWTRIIIEKPFGRDLLTAQRLNRELARVFRENQTYRIDHYMGKET
                     VQNLLAFRFANGIFEPLWNQKYIDHVQIVVAESLGIGSRAEYYEESGAIRDMVQNHIM
                     QVLCLTTMEPPVSFDADSIRDEKVKVMHAIPPLTLEQVAQDTVRGQYTAGVAGGVQIP
                     DYKKEQGVAPNSTTETYVALKLFVENWRWADVPFYIRTGKALPARSTEVTIQFKRVPH
                     QLYKPSETKGLVPNRLTVRIQPDEGITLKFAAKVPGAARHLASVDMDFTYSTAFGIQS
                     PEAYERLLADAMIGDSTLFIRRDEVEASWRIVDSIINGWKQMPAHVFPYAAGTWGPKE
                     AEDLIERDGRQWDNP"
BASE COUNT          643 a          874 c          868 g          698 t
ORIGIN      
        1 gaaatcatcc caggtgggcg aaccggtggc atcctttgtc ccaccgaagc cgaacggaga
       61 accgggtgat tccagcataa gtgagcgcag catgccagga tagtcgatag cgtactgcat
      121 cacgacattt ccgccaagcg accagcctag caggtgaaac gacgtaaggt tggtcgcgcg
      181 cgcaaaggcg tccaggtcgt cggagaaatc gcgcacccct cgcgttgcgt ctaccggcag
      241 cgtttcgctg tcgccatagc cccgcatatc gggcgcgaag atgcgataac ggccagttgc
      301 cacgagcgcg agcatgaaat cctggaaaaa tgccgaagaa gagcaatttc catgtatcaa
      361 cacaaccggc acgtctccgg tgccagcttc aagataggca acctttagcc tatcggtctg
      421 gaagatatgc tgcgtaattc cgggaagtgg cgctgtcgaa ctcataggta aataacctcc
      481 tcagtatatg cttacgagga tattgtaaca cgtatgggat acgtgtggca tggcgaccct
      541 tgcggtctcc atgccgcatc gaccttacac ctgttccgct tattctgtcc tcagtcctgg
      601 ctgccgtatg gtccaggtgg cgtgggcggc tttttttctt gcggcagcat atacgcttct
      661 ggcgaagtgt agtctcccga agggacaaac tcctggtacg tttgcggcgg aggaggtgga
      721 gtatatccct gtggtggagg tgtggctatg ccctgcggcg ggtaagatgg cctggtatag
      781 gaggggtact gtgacatcgg cggtgagcct gcctgtccct tgccaatagc tccaccgatc
      841 gagccgattc ccagtccaag caaggttgcg cctgccacca ccaccaccag gaacaaaatc
      901 acgcttgcaa tcaccaggct gtttgtgatc gtgatgccct gattcaagtt gctcgcagtc
      961 atctgctgtt gcagttgcac gcgaaacgca tcaatatttg aaaggtagta gacaaccagc
     1021 ggaatggtcg cgatgatgga actgaacagg ccggcgaaca aacctgccac taaaccggtg
     1081 ctgaccttgc cggtgcgggt tgaggcgcga taacctgcga acccaaccat gaacaagtac
     1141 aggaggacgc tgatgagcaa gcccggcgtc cccaacaaat aggagaggac gatttcggca
     1201 actccgagtg tcaagccgaa aagcagtcca ttttgtaagg ctggtttgcc caggcgagcc
     1261 tgctgcatat gcttctcccg gcgtctttca agtcgatacg atcaacttgc atcatgatac
     1321 agcaatttct atgaagaaac aaccctggta atgacttacg gattgtccca ctggcgacca
     1381 tcgcgctcga tcaggtcctc ggcctctttc ggcccccacg tgcctgcagc gtaggggaag
     1441 acatgagcgg gcatctgctt ccagccgttg atgatgctgt ccacgatacg ccaggaggct
     1501 tccacctcgt cacgccggat gaagagcgtg gagtcgccga tcatagcgtc ggctagcagg
     1561 cgttcatagg cttcgggcga ctggatgccg aaggcagtgc tgtaagtgaa atccatgtca
     1621 accgaggcaa ggtgacgggc cgctccggga accttcgcgg cgaatttgag cgtaatacct
     1681 tcatcgggtt ggatgcgcac cgtcaggcgg ttcggcacca gtccctttgt ctcggagggc
     1741 ttgtagagct ggtgtggaac gcgcttgaac tggatcgtta cctcggtgct gcgcgcgggc
     1801 agggctttgc cggtgcggat gtagaacggc acatcggccc agcgccagtt ctcgacaaaa
     1861 agcttgagcg ccacatatgt ttccgttgtc gaattgggcg cgacgccttg ctccttcttg
     1921 tagtcaggta tctgcacacc accggccact cccgccgtat attgcccgcg caccgtatcc
     1981 tgcgccactt gctccagtgt cagtggcggg atggcgtgca tgactttgac cttctcatcc
     2041 cgaatagaat cggcatcgaa ggagaccggc ggctccatcg tcgtcagaca cagcacctgc
     2101 atgatgtgat tctgcaccat gtcgcggatc gctcccgact cttcgtagta ttccgcacgc
     2161 gagccgatgc ccaggctttc cgccacaaca atctgcacgt gatcgatata tttctggttc
     2221 cagagtggct caaaaatgcc gttggcgaag cgaaaagcca gcagattctg caccgtctct
     2281 ttgcccatgt agtggtcgat gcgataggtc tgattctcgc ggaagacgcg cgccagttcg
     2341 cggttcaggc gctgcgccgt gagcaggtcg cgtccaaagg gtttttcgat gatgatgcgc
     2401 gtccaacttt cctcaccgtt gttgtcgttc gattgacgtg ggcgagcgag tcctgcgcca
     2461 ccgagctggt ggatgatctc cgagtccagc gtgggtggcg tcgccaggta gtagaggcga
     2521 ttgccctgcg ttccctcttc tgcgtccagt ttttcgagca aatccgccag cttcgcatat
     2581 ccctcgcggt cattgaagtc tgcctggcaa taaaatagct tttgcgcgaa ggctttctgc
     2641 gccttctcgt ccaacttatc gtcatccggc ataaactggt tgatcgaatc aagcgccatt
     2701 gcccgccatt gctcgtcggt catcgggcgt cgcgcgaagg ccaccacgct gaagccctgt
     2761 ggcagtgggt gatcgtgcgc caggtgcgcc agcgtcggca gcaacttgcg ctgcgtcaaa
     2821 tcgccggtcg ctccaaaaat cacgacggcg catggctccg gcgtctgccc atcctgccat
     2881 atcgaatgtt gtttcaacgg aatgttcgct tgcgtttgtt gtgtcataaa agccactcct
     2941 cacaaatctg gtttattatg cttttgattg agtataccgc acgttacaat acacgtgcat
     3001 cccctccgtt ttgtctccca aatttgggaa atgttaccga ccaaccccaa ttctgtcatg
     3061 ctgagcgcag cgaagcatct cgg
//
LOCUS       DASZAK010000735         1840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_550694, whole genome shotgun sequence.
ACCESSION   DASZAK010000735 DASZAK010000000
VERSION     DASZAK010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550694"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..>1840
                     /locus_tag="VGT44_12925"
     CDS             102..>1840
                     /locus_tag="VGT44_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_271413143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:666..668,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2581750.1"
                     /translation="MQERTLLPEPMQSMVSDVMEIGSRVQPFSDESRDKTPRIREAKV
                     VHSVCPYCAVGCSLNVYVKDGKVLDIEGDPNSPINHGTLCPKGAATFQYTINPNRLTQ
                     ILYRAPFSDHWEVRSLAWAMEQIAQRIKKTRDETFVERLPDGREVNHTLGIASLGGAT
                     LDVEENYLMKKLLTGGLGIVSIENQARIUHSSTVPGLGTSFGRGGATTFPQDLVNSDA
                     ILIMGSNMAEAHPIAFANVVKAKELGAKVIHVDPHYSRTSALANLYVSTRAGSDIVFL
                     GAIIRHILETGGYFHEYVANYTNAATLVRQDFRDAEDLDGLFSGYDPASETYTDQGSW
                     DYERDSDGRPLTDPTMQDPRCVLQVMRRHFARYTPEMVEEVCGVSREQWLLVAQTLIE
                     NSGRERTAAICYAVGWTQQSKGVQIIRAAAIIQLLLGNMGRPGGGIMALRGHASIQGS
                     TDVPTLYDLLAGYMPQPSALLTDRPPPQQTLQEYIDTTGQKYGWWSNTPAYIRSLLQA
                     WYGDAADEEDGNCAYRLVPKITGDHSHLVTSYAMLDGKVKGYLLFGQNPAAGSTNSIM
                     QRKALEQLDWMVVR"
BASE COUNT          393 a          588 c          537 g          322 t
ORIGIN      
        1 cgtcccgacg acgggaggaa cagccatctc cgtgatacaa tagagagagc aggacagccc
       61 tgcgcggata accccatctg cctggaaaag gatgatgttc tatgcaagag cgcacacttc
      121 ttcctgagcc aatgcaatcg atggtctccg atgtgatgga gatcggctcg cgcgtccaac
      181 cattcagcga tgagagccgc gacaaaacgc cgcgcattcg cgaagcgaag gtggtacaca
      241 gcgtctgccc ctactgcgcg gtgggatgca gcctgaacgt ctatgtcaag gatggcaagg
      301 tccttgatat cgagggcgac cccaacagcc ctatcaatca tggcacgctc tgtcccaagg
      361 gggcggccac cttccagtat actatcaacc ccaatcgcct gacacagatc ctgtatcgcg
      421 ccccgttcag cgatcactgg gaggtcaggt cgcttgcctg ggccatggag cagatagcac
      481 agcgcatcaa gaagacacgc gatgagacct ttgtggagcg actgcccgat gggcgcgagg
      541 tgaaccatac gctgggcatt gcttcgctgg gcggcgcaac gctggatgtg gaagaaaact
      601 atttgatgaa gaagctcctc accggcggat tgggcatcgt ctccatcgag aaccaggccc
      661 gtatatgaca cagttccacc gtacccggtc tgggtacatc gttcggacgg ggaggcgcga
      721 ccaccttccc gcaggacctg gtcaatagcg atgccatcct gattatgggc agcaacatgg
      781 ccgaggcgca ccctatcgcc ttcgccaacg tcgtcaaggc caaagaactg ggcgcgaagg
      841 tcatccacgt tgacccgcac tattcgcgca cttcggcgct ggcgaacctg tacgtatcga
      901 ctcgcgctgg aagcgatatc gtctttctgg gcgcgatcat tcgccatatc ctggaaaccg
      961 gcggctattt tcacgagtat gtggccaact acaccaacgc cgctacgctg gtgcggcagg
     1021 attttcgcga tgcggaagac ctggacggcc tcttctccgg ctatgatccc gcctcggaga
     1081 cctataccga ccagggcagt tgggactacg agcgcgacag cgacggcagg ccgctcaccg
     1141 acccgaccat gcaggaccca cgctgcgtct tgcaggtcat gcgccgccac ttcgcgcgct
     1201 acacgcccga gatggtggag gaggtctgcg gcgtatcgcg cgagcaatgg ctgctcgtcg
     1261 cgcaaacgct gatcgagaac tccgggcgcg agcgcaccgc agcaatctgc tacgccgtgg
     1321 gctggacgca gcaatcgaag ggtgtgcaga ttatccgcgc cgccgccatc atccagctcc
     1381 tgctcggcaa tatgggccgg cccggcggcg gcatcatggc gctgcgtggt cacgcctcca
     1441 tccagggttc caccgacgtg ccaaccctgt acgatctgct cgcgggatat atgccccagc
     1501 cgagcgccct gctcaccgac agacctcctc cgcagcagac gctgcaagaa tacatcgaca
     1561 ccaccgggca gaaatacggc tggtggtcga acacgcccgc gtatatccgc agcctgctgc
     1621 aagcctggta tggcgacgcc gccgacgagg aagacggcaa ctgcgcctat cgtctggttc
     1681 ccaagatcac cggcgaccac tcccatctgg tcacgagcta cgccatgctg gacggcaagg
     1741 tcaaaggcta cctgctcttc gggcagaacc ctgccgccgg cagcaccaac tccattatgc
     1801 agcgcaaagc attggaacag ctcgactgga tggtcgtgcg
//
LOCUS       DASZAK010000736         2175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_18804, whole genome shotgun sequence.
ACCESSION   DASZAK010000736 DASZAK010000000
VERSION     DASZAK010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18804"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..>2175)
                     /locus_tag="VGT44_12930"
     CDS             complement(71..>2175)
                     /locus_tag="VGT44_12930"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEV2581751.1"
                     /translation="LLGVTANEATRTHHWRHDVPHAAYLISLVVGDFAVIEDHYKNIP
                     VTYYVRPDRKDDAPLYMGKTPEMIRFFSEYTGVEYPYDKYAQTVVEIYTGAMEHTTAT
                     THSFSLLLDKRASLDVNLVPVVAHELAHQWFGDLLTCRDWSNGWLNEGFATYFEELWG
                     EHDQGADYFKQSMLDLKQGYLEEDAHYRRPIVYYVYHDDGFELFDAHLYNKGAWVLHM
                     LRHQLGEAAFKRALHAYISRYREREVITADLERTFEDVTGRSLAQFFQQWVYKGGYPA
                     FEVNYAWDSEHNMAKVKIKQTQRIDDLTSCFVTPVDLAFTVPTSDEAAKDDNTTETRI
                     VELRVTVGEDEQVEQTFYVPLERQPVMVRFDPNGWLLKTLKFERPASMMRYQLARDPD
                     VLGRIEAAKALGEVGDDESIEALKQALLADAFWGVRNAAASALGVIGTRKAQDVLLQA
                     LQELDPAQFSRVRAAIARTLGQFQAPAQAELAERSAQALGALLEKGDVSYLVESASAQ
                     ALGRTRTAGSVDQLVKLIERPSWMNIVQRGIFNGLAVTGEDRAVDYIAAYLGETHVVD
                     GHREANHPTMRSAAARGMLALGEHRYLYSEEARQRAVTALSQAVEHDSWEPVRAISAL
                     ALMSLGEKRAINVLERIARHELDSTTQRDMRLAAYTLRTGDKTDEQLQQLRKDLDQVR
                     EENRKQKEQLEALESRVK"
BASE COUNT          444 a          638 c          586 g          507 t
ORIGIN      
        1 cgagtctacc gggcgaggct tgccctcgtc cggttaccat atctactatc cttgttcctg
       61 gttaccaaat ctactttacc cttgactcga gagcttcaag ctgctctttc tgcttgcggt
      121 tctcttcacg gacctgatcg aggtctttgc gcaactgttg taattgctcg tcggttttgt
      181 cgccggtgcg gagcgtgtag gcagccaggc gcatgtctcg ctgcgtggtg gaatccagtt
      241 cgtgacgggc gattcgctcc agcacgttga tagcacgttt ctctcctagc gacataagcg
      301 ccagggcgga aatagcacgt accggctccc agctatcatg ctcgacggct tgactgagcg
      361 cggtgactgc gcgttggcgt gcttcctcgc tataaaggta ccggtgttct cccagcgcca
      421 acataccccg ggccgcggct gagcgcatag tcggatggtt cgcttcccta tgcccatcga
      481 caacgtgcgt ctctcctaga tacgctgcaa tataatcaac cgcgcgatcc tcccctgtta
      541 cagctaatcc attgaagatg ccgcgctgaa cgatgttcat ccacgatggg cgctcaatca
      601 gcttcaccag ttgatcgaca cttccagcgg ttcgtgtcct gcccaatgct tgcgcggacg
      661 cggattcaac aagatagctg acatcaccct tctcaagcaa ggcgccgagg gcctgcgcgg
      721 aacgttcggc aagttctgcc tgcgctggcg cctggaactg tcctaatgtc ctggcaatcg
      781 ccgcgcgcac acgcgagaac tgtgccggat ctagctcctg gagggcttgt aagaggacat
      841 cctgtgcttt cctggtacca atgacgccca atgctgaggc ggcggcattg cgcactcccc
      901 agaatgcatc ggcgagcaat gcctgcttca gcgcttcgat actttcatcg tcacccacct
      961 cacccaatgc ttttgccgcc tcgatgcgtc caagcacatc tggatcgcgc gcaagctggt
     1021 agcgcatcat gctggcgggt cgctcaaact tgagcgtttt gagcaaccag ccgttggggt
     1081 cgaagcgcac cattacaggc tggcgctcga ggggcacgta gaaggtctgc tccacttgct
     1141 catcttcacc taccgtaact cgcaactcga ctatgcgggt ctctgtcgtg ttgtcgtctt
     1201 tagctgcttc atccgaggtt gggacggtga aggccagatc aacgggggta acaaaacacg
     1261 atgtcagatc atcgatccgt tgtgtttgct tgatcttgac cttcgccatg ttgtgctcgc
     1321 tatcccacgc atagttcact tcaaacgccg gatagccacc cttgtaaacc cactgttgga
     1381 agaactgcgc caggctgcga cctgttacat cctcgaaagt ccgctccagg tcggctgtga
     1441 ttacctcccg ctcgcgatag cgcgagatat aggcgtggag agcgcgtttg aaggccgctt
     1501 ctccaagttg atggcgcagc atatgcagga cccaggcgcc tttgttgtac agatgagcgt
     1561 cgaacaactc aaaaccatca tcatgataga catagtagac gatgggacgg cggtaatgag
     1621 catcctcttc caggtaacct tgcttgagat cgagcatcga ctgcttgaaa tagtcggcac
     1681 cctgatcgtg ttctccccac agttcctcaa agtaggtcgc aaagccctcg ttcaaccagc
     1741 cattggacca gtcgcgacag gtcagcaggt caccgaacca ctggtgcgcg agctcgtgcg
     1801 ctaccacggg cactaaatta acatccaacg aggcccgctt gtcaagcagc agggagaaac
     1861 tatgcgtggt cgcggtggtg tgttccatcg cgccagtata aatctcgaca accgtttgcg
     1921 cgtacttatc atagggatac tcaacgccgg tatactccga gaagaaccgg atcatttcgg
     1981 gcgtcttgcc catatacagg ggcgcatcgt ccttgcgatc aggccgcacg tagtacgtga
     2041 cagggatatt cttgtagtgg tcctcgatga ccgcgaagtc gccgacaacc agtgaaatca
     2101 ggtatgccgc gtggggcaca tcatgtctcc aatggtgtgt cctggtagct tcgttagcgg
     2161 tgacgccgag caggt
//
LOCUS       DASZAK010000737        10143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_516809, whole genome shotgun sequence.
ACCESSION   DASZAK010000737 DASZAK010000000
VERSION     DASZAK010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 10143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10143
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516809"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VGT44_12935"
     CDS             complement(<1..1194)
                     /locus_tag="VGT44_12935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581752.1"
                     /translation="MPEQWFRDYIQLQFRMDWHIRKFTESRFVDFYYGPPEWKAAVES
                     EAETPAPDLVRASMALLDTLPAQGFEARRANYLSKQLVALETVCRKLSGETFTLEDEV
                     QRCFDIRPEWTPETRFEEAHALFEVALPGEGSLYEHMEALRTRFEVARDKAALLPGLM
                     RLAMDEARRRTRAFLDMPPQEEVEMETVSDKLYGGENWYLGNYRSHVDVNTDLPLTIN
                     SLIDLVCHEGYPGHHTEFVLKERLLYRELGYIEESIAPIIGPRSVVSEGIATSAFETI
                     FTHAEAEQWAEEHLYPAAGITPVSYDMEKLARARELMSGIFGNARFMLSEGRPENEVI
                     RYLVKYSLYPEEYEQKEVDFMKDPFREGYIFTYAYGKQLLKPWLAGPDRQQVFKRLLT
                     EQLCPS"
     gene            complement(1203..2795)
                     /locus_tag="VGT44_12940"
     CDS             complement(1203..2795)
                     /locus_tag="VGT44_12940"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEV2581753.1"
                     /translation="MTTSERTPQETSAPKGTQEKLEELHRLKEAAMLGGGVKRIEAQH
                     KKGKLTARERVDLLLDPGTFNELDMFVTHRSTDFGLDEQRIPGDGVVTGYGQIDGRLV
                     YVFSQDFTVFGGSLSEAHSEKICKIMDHAMKNGAPVIGLNDSGGARIQEGVVSLGAYA
                     DIFLRNTLASGVVPQISAIMGPCAGGAVYSPAITDFIFMVEQTSYMFVTGPNVVKTVT
                     HEDVTFEDLGGSMVHNSKSGVAHFSCPTEPDCIRMIRQLMSYLPSNNLEDAPRITPKD
                     SPTRADEALNSIIPDNPNKPYDIKSVIKLIVDDSDFFEVQEYFATNIVVGFARLNGRS
                     VGIVAQQPGALAGVLDINSSDKGARFVRFCDCFNIPIITFVDVPGFLPGVGQEHGGII
                     RHGAKLLFAYCEATVPKITVITRKAYGGAYDVMNSKHVRGDLNYAWPTAEIAVMGAEG
                     AVNILFKDEIDKAANPEQRRKELIAEYTEKFNNPYIAAARGYIDEVIEPRLTRTKLIT
                     GLEMLKNKRDSNPPKKHGNLPL"
     gene            4938..6029
                     /locus_tag="VGT44_12945"
     CDS             4938..6029
                     /locus_tag="VGT44_12945"
                     /inference="COORDINATES: protein motif:HMM:NF023225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HEV2581754.1"
                     /translation="MILLMWMALVAPFGFSIAHAAGGTAQFGLQPVVYDPSNSLTKSY
                     FIFDSKSGVIVKSSVRVTNAGTAAGSVALYPVDATTAQTSGAVYLNQNDPRRDVGAWI
                     TLGTQQLTLNPGQTQVVPFQLTIPGTVRPGQHLGGIVAENLTQQQSSTQAQNGKNTST
                     FQIVVKNLTIIAVQVNLPGTPVEQLAGTGVQAGGENGYQRLLLGLSNTGNVMLKPYGT
                     LQVANSQGQVVKNISLKLDTFLPQTAINYPVAITGQGLAAGDYQATLTLTYGHGQVLH
                     YTTKFTITQQQITQAFGGSSSKTQAPPGLGGDVFAGMPLWQLVLVAGGVLAILWVVGN
                     MVYRRLSVSKAKQAKSQGGSSQFKTPTFR"
     gene            6042..6632
                     /locus_tag="VGT44_12950"
     CDS             6042..6632
                     /locus_tag="VGT44_12950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581755.1"
                     /translation="MRVLKSRYKGYYFIVVMLLAMTTMGSVIAYADSGTATATVRPGG
                     LTVANASNLVSLSLANKKKVRMVNYSLPITVIDARGSGAGWNLMITSTTFQLVEHDKH
                     ASTNQLPANASSVVGVSASCGTNSTCTGPTNGVIYPLLVPAGTTPPPPVKFFDATTQS
                     GLGKFNIVMMVNVTVPASTERGIYTSTLTITIASGP"
     gene            6712..8760
                     /locus_tag="VGT44_12955"
     CDS             6712..8760
                     /locus_tag="VGT44_12955"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2581756.1"
                     /translation="MAITSQTAVSTENRVAGWLTPDEFSILEAVCDTLLPSLEPPMGS
                     SESVAAYYRRSARDLNVARLIAETLAQENEDEQKKLHRLFALLAGPLGGLLLIGSPRP
                     FKSLSQPRREKYLVAMANSPVGQLRQGFQAIKRLAGFVYFAAPDATGVNPNWAALDYD
                     APPPPPLDAPQPISPLRITADTTLEADVVVIGSGAGGGLVAGELAMAGKSVIVLEKGG
                     YNNESNFTYQEAQATPELYLKRGTLTSKDLGVIVLAGSTLGGGTIVNWMTSFRTPPEI
                     LDEWARSSGISDLTGSALQASFAAVEQRIGVAPVADHNRQNQLLYDACVKSGYHADVL
                     RNNAIGCEQRCGSCGFGCRYGCKQSTMKTYLQDAYDHGARIIVRCSAERVLIENGKAA
                     GVEATVTDTETGSSYKLTARAKVVIVAAGSIHSPALLLRSGLKNPHIGRHLHLHPVLT
                     VTGIYPEKVYSWKGVMQSAYSNEFGALDGNYGYKLEVPPAHPGLIGMSTPWFSPRDYR
                     EQMLQTANVATFIILARDRGEGVITVDRFGEPVINYVISVYDRQHLKHGLRHAARLHF
                     AAGASSVITLHNKKVRLDRPASGEVSEQMYHTYARQIERSGMNPNRVLAFSAHQMGTC
                     RMGADPKDAVVDANHEVYGVKGLFVCDGSVFPSASGVNPMLSIMGLTHRAAQYIKTVI
                     "
     gene            complement(8804..9370)
                     /locus_tag="VGT44_12960"
     CDS             complement(8804..9370)
                     /locus_tag="VGT44_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581757.1"
                     /translation="MGILVVLIKLISILAGAAFIIGLVGGWLTAGGTNVSHGIRPSRT
                     EPGSYVFTRIETTDGPWDYNHVFWIICGVGLVIALAMLGLAVLTGANTYMVANSELSA
                     FCEEINVNAPDYQTAYGRLSDNIQNSYSEADFVKYLHDHELLSCTASDNTISFPLSGS
                     VFIACDNSTSWGILGEVRINTFPFFGGQ"
     gene            9716..>10143
                     /locus_tag="VGT44_12965"
     CDS             9716..>10143
                     /locus_tag="VGT44_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581758.1"
                     /translation="MKNRSSTNHASQNGQNGSVGKTGQADKAEKTDKTSKATGDKKQE
                     EEEQFKIRHIFAAFASLPRVMKLVWSTNARLTAAMLAISILRGFLPAISVYITKQVID
                     SVFKAILSVTHDTRLVWLFVGLQLAANLLDRLLSTLSNIV"
BASE COUNT         2271 a         2827 c         2704 g         2341 t
ORIGIN      
        1 agacggacac agctgctctg tcaagagtcg cttgaagacc tgctgccggt ctgggccagc
       61 gagccagggc ttcaacaact gcttaccata cgcataggtg aagatgtagc cttcgcggaa
      121 gggatctttc ataaaatcca cctccttctg ctcgtactcc tctggataca gcgagtactt
      181 caccaggtag cgtatgactt cgttttccgg acgaccctcg ctcagcataa agcgcgcgtt
      241 gccaaagatg ccgctcatca gctcccttgc cctggccagc ttttccatgt catacgaaac
      301 aggcgtgatg ccggctgccg ggtaaagatg ctcttccgcc cactgctctg cttcagcatg
      361 ggtgaagatc gtctcaaagg ccgaggtcgc aatcccttcg ctgacgacgc tgcgcgggcc
      421 gatgatgggc gcgatggatt cctcgatata gcccagttct cggtaaagta gccgctcttt
      481 cagcacgaat tcggtgtgat ggccggggta gccttcgtgg caaaccaggt ctatcaggct
      541 atttatggta agaggtagat cggtgttcac atcaacgtgc gagcgatagt tacccaggta
      601 ccagttctcg ccgccataga gcttgtccga gacggtctcc atctccactt cttcttgcgg
      661 cggcatatcc agaaaagcgc gcgtgcggcg gcgcgcctcg tccattgcca gccgcatcaa
      721 gccgggcaac agcgccgcct tatcccgcgc cacctcaaag cgcgttctca aagcctccat
      781 gtgctcgtac aggctgcctt cgccgggcag cgccacctcg aagagcgcgt gagcctcttc
      841 aaagcgcgtc tccggcgtcc actccggtcg aatgtcgaag cagcgctgta cctcgtcttc
      901 cagcgtgaag gtttcgccgc tcagcttgcg gcagaccgtt tccagcgcga cgagctgttt
      961 cgacaggtag tttgcacggc gcgcctcgaa gccctgcgcg ggcagcgtgt caagcagcgc
     1021 catcgatgcg cgcacgagat caggcgcggg cgtctccgcc tcgctctcga cggcggcctt
     1081 ccattccggc gggccataat aaaagtccac aaagcggctc tcggtgaatt tgcgaatgtg
     1141 ccagtccatg cggaattgca actggatgta atcgcgaaac cattgttcgg gcatatttac
     1201 tcctacagcg gtaaattccc gtgcttcttc ggcggattgc tatcccgctt attcttgagc
     1261 atctccaacc ccgttatgag ctttgtgcgg gtgaggcgcg gctcgatgac ttcgtcgatg
     1321 tagccacgcg cggcggcgat gtaaggattg ttgaatttct cggtatattc ggcgatcagt
     1381 tctttgcggc gctgttccgg gttggcagct ttgtcgatct cgtccttaaa gaggatgttg
     1441 accgcgccct ccgcgcccat cacagcgatt tccgccgttg gccaggcgta gttgaggtcg
     1501 ccgcgcacat gcttgctgtt catcacgtcg taggccccgc cgtaggcttt gcgcgtaatc
     1561 acggtaatct tcggcacggt cgcctcgcaa taggcaaaga gcagcttcgc gccgtgccgg
     1621 atgatgccgc cgtgttcctg tccgacgccc ggcagaaagc ccggcacatc gacgaaggtg
     1681 atgatgggaa tgttgaagca gtcgcagaaa cgcacgaaac gcgcgccctt gtcggacgag
     1741 ttgatgtcca gtacgcccgc cagcgcgccg ggctgctgag ccacgatgcc gaccgaacga
     1801 ccgttcaggc gcgcgaagcc gacgacgata ttggtcgcga aatattcctg cacctcgaag
     1861 aaatcgctat catccacgat cagcttgata acggacttga tgtcatacgg cttgttcggg
     1921 ttgtccggga tgatgctgtt gagggcttca tcggcgcgcg tgggcgagtc cttgggcgtg
     1981 atgcgcggcg cgtcttccag gttgttggac ggcagatagc tcatcaactg gcggatcata
     2041 cggatacagt ccggttcggt cgggcaggag aaatgcgcca cgccgctttt gctgttatgc
     2101 accatcgagc cgcccaggtc ctcaaaggtg acatcctcgt gtgtgacggt tttgaccacg
     2161 ttcggccccg tcacgaacat gtaactcgtc tgctcaacca tgaaaataaa gtcggtgatg
     2221 gccggcgagt agaccgcgcc gcccgcgcac ggtcccatga tggcggagat ctgcggcacg
     2281 acgcccgacg ccagcgtgtt gcgcaagaaa atatcggcat acgcgcccag gctgacaacc
     2341 ccttcctgga tgcgcgcccc accggagtcg ttcaggccga taaccggcgc gccgttcttc
     2401 atggcatgat ccataatctt gcagattttc tcggaatgcg cctccgacag cgagccgcca
     2461 aagacggtga agtcctgcga gaagacgtac accagccgcc catcaatctg cccatagccc
     2521 gtcaccacgc cgtcgcccgg tatcctctgc tcgtccagcc caaaatccgt cgagcgatgc
     2581 gtcacgaaca tatccagttc gttgaacgtg ccggggtcta acaacaggtc aacacgctcg
     2641 cgcgccgtca atttgccctt tttatgctgc gcctcgatac gtttgacgcc gccgcctagc
     2701 atagcagctt ccttcaaccg gtgcagttct tccagcttct cctgcgtgcc tttcggggcc
     2761 gatgtctcct gcggggtgcg ttcgcttgtg gtcatctctg ttctcgctcc atacctacgt
     2821 ctttgtggtt cctatgcata actgatatgg cagtatagca cctgggcgag aggcgtgtct
     2881 aacggggctg cgcgcacaag cgcgtctcac ttcaagatgc gcaatatcta tggtggggat
     2941 ctgatgctag accaggttta gataaatgaa acgagatgaa acagcttgat atagagtgca
     3001 tttattacac tacaataaaa attacattgt tgtaatatta tcgataaaac atctctaaaa
     3061 gtattgacta ttttagattt atcattataa tctcaggtat aggcaagtta ctggatatat
     3121 gggggtatat ccaggtacga ttttcagagc aaagtagttt atcacgtggc atgtgggtga
     3181 tccctccaat tttgcaagga agctccaaac tgtgttgatt taaggaatga ggcggcaatc
     3241 ccacggcttc ttctttagga agctctgacg tgagacatca catatatgtg tatcctatcg
     3301 agtaatgtct actttgcctg aaagatcagt atcctgagct agaagcttac agtgcgagag
     3361 ttctaccccg aagccccagc cttgccaggt ttaaattttg gctgtgacct gacaaccagg
     3421 tatgcggcca ctacaacctc aaaaaggggg ataatcttat gggcttactc aaaaagcgat
     3481 ctacacgttt ctacctcggg tttatcctga tcctcgcgat ggcgctgggc agttcggcca
     3541 tcgccgcctt cgctagtggg ggcggcaccg ctactgctac cctcagcgct ggacagttga
     3601 cggagagcga tacctttgct ccaacgtttc ctactacaca attgaccggt gcggatcaga
     3661 ctttaaccaa agctggcggt ctgtctatgc aggtaactga cgcgacaggc tctggagctg
     3721 gttggaggac gacaattaca gctgcgtcgt tcgtgtgcca aagcaatact ccccaggatc
     3781 catgctttac cgctggccct agcaatactc cggctactct ggatcccagt ggcacacctt
     3841 tgtctctcac taatatgtct gcggcatgcg tcgcgggcaa tggttgcact gcaccagtct
     3901 ctagcggtac tgttccaact caagtatatg caagtggcac tgcagcgcct ttttacagtg
     3961 ataaagccaa caatggcctg ggcattatca acgtcacagc ggatctcaat ttgttcgtcc
     4021 ctggcaatac atatgcaggc acctataaga gtaccgtcac cctggccgtc gttagcggac
     4081 cgtagttact ttcacgtttt gagccacaag ctctctgaga gggggagagg ggaaagtaac
     4141 tttcctcgtt tccctcctct ccttgtggta acaaaccgat agttggtagg gagcgcttta
     4201 gaccaaacct taaggaaagg agaacgtatg tttcgacatc gaagcaagtt gctgcaagca
     4261 acggttgctc tggtcttttt gactacgaat gccgtgctgc tgtggtcaac aatgggcgct
     4321 tcggctacct acgctgcgtg tactcctgta agtaacgatg tgtgtatcag caccactcca
     4381 gctccatctg gtaccttgat cctgaatggt agagatctga ttctatacta caagctggat
     4441 tttagcctga atagctccag cgccgctacc tggcatgtga ccatcaactc aactcaattc
     4501 gtgactacca gtatagcagg tactccgaca cgtactttgt ctataaatgc ttcaaatgtt
     4561 acagctgtaa cgacggtttc ggcatgcccg gggagtggcg gatcggattg ccccactaac
     4621 gggcttatat atcctgttgc tatgccagta ggtacagtag ttacgttttt ccaaaataca
     4681 aacaagaaca aagggtctgg cacctttaat ctcgaggcta caataggtgt gctgattcca
     4741 gggaacgcct atgcgggtac gtataccagt actatcacga tcacccaggc aaccggccct
     4801 acttaagtcc tatgtaatcg gcgctccata aattttttgg catagtagtt ataaaaggat
     4861 gtctttagat gcaaaatgca ctttccttcc cccactccgg ttttttctcc cggatgatta
     4921 tgggagcgtg tctattaatg atcttactaa tgtggatggc gttggtagcg cccttcggtt
     4981 tctcaatcgc tcatgctgct ggcggtacgg cccagtttgg cttgcaaccg gtcgtttacg
     5041 acccttccaa ctcgctcacc aaatcctact ttattttcga tagcaaatcg ggcgtcattg
     5101 tgaagagtag cgttcgggtg acaaatgctg gtacggccgc gggtagtgtt gcactctatc
     5161 cagtcgatgc aaccaccgct cagacgagcg gagcggtcta cctcaaccag aatgatccac
     5221 ggcgcgacgt gggagcctgg atcactcttg gcacacagca gctgacgctc aatccaggtc
     5281 agacccaggt tgtcccattt cagctcacca ttcctggtac ggtgcgtcct gggcagcatc
     5341 ttggcgggat tgttgcagag aatttgacgc aacagcagag cagtacccag gctcagaatg
     5401 gtaagaatac cagtacattt cagattgtcg tgaagaatct gacgattatt gccgtgcagg
     5461 tcaacctgcc tggcacgcca gtcgagcagt tggcagggac aggcgtgcag gccggtggcg
     5521 aaaatggcta ccaacgtctc ctgctaggac tgagcaacac aggtaatgtc atgctcaaac
     5581 cttatggtac gctgcaggtc gcgaattccc aggggcaggt tgtgaagaat atctcgctca
     5641 agcttgacac cttcctaccg cagactgcca tcaactaccc ggtcgctatt acgggacagg
     5701 gccttgcggc tggtgattac caggcgacgt tgaccttgac atatggacat ggccaggtgc
     5761 tgcactatac gacgaagttc actattacgc agcagcagat cacgcaggca tttggcggga
     5821 gcagcagcaa aacccaggcg cctccagggt tggggggcga tgtattcgcc gggatgccgc
     5881 tctggcaact tgtcctggtg gcgggcggcg tgcttgctat cctttgggtt gtgggtaata
     5941 tggtttatag aaggctttct gtatcaaagg caaaacaggc gaaaagccag ggtggttcat
     6001 ctcagtttaa gaccccaact ttcaggtgaa gaggtccgct tatgagagtt cttaagagtc
     6061 gctacaaggg atactatttc attgttgtca tgctgctcgc tatgacaaca atgggttccg
     6121 tgatcgcgta tgccgactca ggaacggcta cggctaccgt cagacctgga gggttgacgg
     6181 tagcaaatgc ttcgaacctg gtgagtctct ccctggcgaa caagaaaaag gttcggatgg
     6241 tcaactatag cctgccaatc accgtgattg acgcgagagg ctcaggggct ggctggaatc
     6301 tgatgattac atccacaaca ttccaactgg tggagcatga taagcatgcg tccacgaacc
     6361 agttgccagc caatgcttcg agtgtcgtag gggtaagtgc gtcctgcggt acgaattcta
     6421 cctgcaccgg cccaacgaat ggtgtcattt acccgctcct tgtcccggct gggactacac
     6481 caccgccgcc cgtcaagttt ttcgacgcca caactcaatc gggcctggga aagtttaata
     6541 ttgtgatgat ggtcaacgtc accgttcccg ccagtacgga acgcggtatc tatacgagta
     6601 ccctcactat aacaattgcc agtgggccgt aagcctagct gcaagtggat gtgctatact
     6661 tcctccatat attgccatcc ataactactg actccaccaa agggggctat catggccatt
     6721 acctcgcaaa ccgccgtttc gaccgagaat cgcgtcgctg gctggctgac tcccgacgaa
     6781 ttcagcatcc ttgaagcagt ctgcgatacc ctcctgccct cgcttgaacc tcctatgggg
     6841 agttcagaga gcgtcgcagc ctactaccgg cgcagcgcgc gtgatctgaa tgtcgcccgg
     6901 ctcatcgccg aaacgctcgc gcaggaaaat gaggacgagc agaagaaact acaccgcttg
     6961 tttgcgctgc tggccgggcc gctcggtggc ttattgctga tcggtagccc tcgccccttt
     7021 aaatcgctgt cgcagcccag gcgcgagaag tacctggtgg caatggcgaa cagcccggtg
     7081 ggacaactgc gacagggctt tcaggccatc aagcggctgg ctggcttcgt ctacttcgcc
     7141 gctccggatg cgaccggcgt caaccccaac tgggccgccc ttgactacga cgcgcccccg
     7201 cccccgccgc tggacgcgcc gcagccgatt tcgcccctca gaatcacagc agacaccacg
     7261 ctggaggccg acgtggtggt gattggctcc ggcgctggcg gtggactggt cgcgggcgaa
     7321 ctggcgatgg cgggcaaaag tgttattgtg ctggaaaagg gcggctataa caacgagtcc
     7381 aatttcacgt accaggaagc gcaggccacc cccgaactct atttgaaacg cggtacgctc
     7441 acctcaaaag acctgggcgt gattgtgctg gccggcagca cactgggcgg cggcacgata
     7501 gtaaactgga tgacatcatt caggaccccg ccggaaatcc tggacgaatg ggcgcgctcc
     7561 tccggcatct ccgatctgac cggctccgct ttacaggcca gcttcgccgc cgtcgagcag
     7621 cgtatcggcg tcgcgccggt cgccgaccat aacaggcaga atcaactgct ctacgatgcc
     7681 tgtgtgaagt cgggctatca cgcggatgtg ctgcgcaaca atgctatcgg ctgcgagcag
     7741 cgttgcggct cctgtggttt tggctgtcgc tatggttgca agcaatccac gatgaagacg
     7801 tacttgcagg acgcctacga tcatggcgcg cgtatcatcg tgcggtgcag cgccgagcgg
     7861 gtattgatcg agaacgggaa agccgctggt gtagaggcta ccgtaacgga taccgagaca
     7921 ggcagcagct acaagctgac agcgcgcgcg aaggtcgtca ttgtggcggc tggctcgatc
     7981 cactcgcccg cactgctgct gcggtcggga ctgaaaaacc cgcatattgg gcgacacctg
     8041 cacctgcatc cggttttaac cgttaccggc atctatccag agaaggtcta tagctggaag
     8101 ggcgtcatgc aatcggccta ctcgaacgag tttggcgccc tcgacggcaa ttatggctac
     8161 aagttggaag tgcctccagc tcatcccggc ctgatcggca tgtccactcc ctggttttcg
     8221 ccgcgcgact accgcgagca gatgctgcaa acggccaacg tcgccacgtt catcatcctg
     8281 gcccgcgacc ggggagaagg cgtcatcacc gttgaccgct tcggcgaacc cgtcattaat
     8341 tacgtcatct cggtctatga ccggcagcat ctcaagcacg gcctgcgcca cgcagcgcgt
     8401 ctgcacttcg ccgccggtgc cagttccgtt atcaccctgc acaacaagaa agtccgcctc
     8461 gaccgccctg caagcggcga agtgagcgag caaatgtatc atacctatgc ccgtcagatc
     8521 gagcggagcg gcatgaaccc caaccgcgtc ttagctttct ccgcccacca gatgggtaca
     8581 tgccgcatgg gcgctgaccc taaagatgcg gtggtagacg ccaatcacga ggtctacggc
     8641 gtgaaggggt tgttcgtgtg cgacggcagc gtctttcctt ccgcatctgg cgttaacccg
     8701 atgctatcga tcatggggct gacgcacagg gcggcgcagt atattaagac ggttatataa
     8761 ctgtatttcc cattaaatct gcatagttta gcgtgcagag agcctactga cccccaaaga
     8821 acggaaaagt attgatcctg acctcaccca aaattcccca ggaggtgcta ttgtcgcatg
     8881 cgataaatac gcttccagag agagggaagg aaatggtatt gtcacttgca gtacatgata
     8941 atagctcatg atcatgcaga tactttacaa agtccgcttc actgtatgag ttttggatgt
     9001 tgtcagagag gcgtccatac gctgtctggt aatctggagc gttaacatta atctcttcgc
     9061 agaaggctga aagttcggaa ttagcgacca tataagtatt agcgcccgtt agaaccgcga
     9121 gacccagcat agctaatgca atcactaagc cgactccaca tattatccaa aagacgtggt
     9181 tataatccca aggtccatct gtcgtttcga tcctcgtgaa tacatagctg ccaggttcgg
     9241 tacgtgatgg acgaataccg tggcttacat tcgttccccc tgcggtaagc cagcctccta
     9301 ccaatccgat gataaatgcg gctcctgcaa gtattgagat tagcttgatg agaactacta
     9361 gtatacccat ttattacctc cagagtggat tacgatgaag cataccaatt gcttatttcc
     9421 cctccatact tattagaagg gatgaattct caatcgaagc gacgttggcc aactgtttcc
     9481 gtaacacttt tagtactcct ttcgataact tgagggagcc atacattgaa gccaatctat
     9541 tgaacaacgg ggaaagaggg attcaatcct aactactgat gagttttgcg gagagatatt
     9601 acaaaaattg acacaggatg tcatatattc ttgcatatag tatatctatg acacgataac
     9661 ttctttctgc gactttttta taattggcct ccatttacgt ctaatacttg agcatatgaa
     9721 aaatcgttcc tcaacaaacc atgccagcca gaacggtcag aacggcagcg tcggcaaaac
     9781 gggccaggca gacaaagcgg agaagacgga caaaacgagc aaggcgaccg gcgacaaaaa
     9841 gcaggaagaa gaagagcagt ttaaaatccg gcatatcttc gctgcttttg ctagcctgcc
     9901 gcgtgtcatg aagctggtct ggtctaccaa tgcgcggctg acggcggcca tgttggctat
     9961 cagcatcttg cgcggcttcc tgcccgccat tagcgtctat atcaccaagc aggtcatcga
    10021 cagcgtcttc aaagccatcc tcagcgtcac gcacgacacc aggctggtct ggctcttcgt
    10081 ggggctgcaa ctggccgcga acctgctgga ccgcctgctg agtacgctca gcaacatcgt
    10141 gca
//
LOCUS       DASZAK010000738         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_90426, whole genome shotgun sequence.
ACCESSION   DASZAK010000738 DASZAK010000000
VERSION     DASZAK010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90426"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VGT44_12970"
     CDS             <1..884
                     /locus_tag="VGT44_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEV2581759.1"
                     /translation="MAQHNLRWAADAVQRHPVKAQTLGVDADETARWIEAAEKMYLPY
                     DEHLGVTPQAESFTEHEVWDFAHTTPDMYPLLLHFPYFDLYRKQVIKQADLVLAMQLR
                     GDMFTPELKARNFAYYEPLTVRDSSLSASTQAVMAAEVGQLDLAYDYLGEAALLDLHD
                     LHHNVKDGLHMASLAGAWTAVVAGLGGMRVQNGSLSFAPRLPSALERLAFHLVFRDCR
                     LRVEVRATEATYRLLDGSPLVVRHHGEEIHLVVNEAITRSIPPVEAGPRPTQPPGREP
                     IAREAQVRPTKISAPGS"
     gene            913..1557
                     /gene="pgmB"
                     /locus_tag="VGT44_12975"
     CDS             913..1557
                     /gene="pgmB"
                     /locus_tag="VGT44_12975"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEV2581760.1"
                     /translation="MQPQAVIFDLDGVLCDTSEYHYQAWKHLADDEGIPFSRAENDQH
                     LRGVGRRESLMFIIRGRQYTENQVQEMMARKNRYYLQLVEQMTPQDLVPGGRELLEQL
                     RSAGIKKAIASGSKNCRLVLDRLGITDLFDGIADGNSITRSKPAPDVFVWAAGRVDVA
                     CNACLGIEDANDGIIAIRTAGMRTLGVGPAERFTPLPDKLLPSFAHVNLQDLIF"
     gene            complement(1564..2481)
                     /gene="rocF"
                     /locus_tag="VGT44_12980"
     CDS             complement(1564..2481)
                     /gene="rocF"
                     /locus_tag="VGT44_12980"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004053 - arginase activity [Evidence
                     IEA];
                     GO_process: GO:0019547 - arginine catabolic process to
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HEV2581761.1"
                     /translation="MNIHFIGVPLDLGAGKHGADMGPNAIRYAGLNLLPSIDEQLRKL
                     GHPLIDEGNLAIPQPRIEQPHIANVHLRNVETILEVCGSLATTVERCCGPDDFPLVIG
                     GDHSLTMGSLTGVVRARGPVGVIWVDAHADFNTDTTTPSGNVHGMSLAALVGIGNTQL
                     VELGGISPKVDPHRVVIIGSRDLDPGEKDFLREVGIHVFTMKEIDRLGMAAIMEQAIE
                     LATPGTNGLHISFDIDAVDPRDAPGTGTKVAGGLTYREAHLALELIADSGKMTSLDIV
                     EVNPTLDMENRTAMLAAELILSAFGKRIF"
     gene            2640..>2854
                     /locus_tag="VGT44_12985"
     CDS             2640..>2854
                     /locus_tag="VGT44_12985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581762.1"
                     /translation="MQSFDWFPLVQRYTAALVRIRSVSPGQGEREVAQAVLKLLQEDG
                     LASSYSEIRLDPLVGDPAGRSNAYAFLR"
BASE COUNT          600 a          875 c          798 g          581 t
ORIGIN      
        1 tcatggccca gcataacctg cgctgggcgg cagatgcggt gcagcgccac cccgtaaagg
       61 cacagaccct cggtgtggac gctgacgaaa ccgccaggtg gatcgaggca gccgaaaaga
      121 tgtacctccc ctacgacgaa cacctgggag tgaccccaca ggctgagtct tttaccgaac
      181 acgaggtatg ggacttcgct cacaccacac cggacatgta ccccttgctc ctgcatttcc
      241 cgtactttga cctctatcgc aagcaggtca tcaagcaggc tgaccttgtc ctcgcgatgc
      301 aacttcgcgg cgatatgttc acgccagagc tgaaggcgcg caacttcgcg tattatgagc
      361 cgctgaccgt gcgcgactcc tcgttgtcgg cctccactca ggcggtcatg gctgctgaag
      421 tgggccagct cgacctggcc tacgattacc tcggtgaggc ggcgcttcta gacctccacg
      481 acctgcatca taacgtgaag gacggactac acatggcctc actggcgggg gcatggacgg
      541 cagtggtcgc gggattaggg gggatgcgtg tacaaaacgg ctcgctctcc ttcgcaccgc
      601 gactgcccag cgcgctggaa cgcctcgcct tccatctcgt tttccgcgat tgtcgcctgc
      661 gtgtagaggt gagagcgacg gaagcaacgt accgcctgct cgacggctcg ccactcgtag
      721 tgcgccatca cggcgaagaa atccacctgg tcgtgaacga ggctatcacc cgctccatcc
      781 cacctgtaga agccggaccg cggcccaccc aacctcctgg gcgagaaccg atagcgcggg
      841 aggcgcaagt gcgccccaca aagatatcgg cacctggcag ttgaggctca tttgcattga
      901 aggaggcaaa atatgcaacc tcaagcagtc atttttgatc ttgacggcgt gttatgcgat
      961 acttcggaat accactatca agcctggaaa catttagccg atgacgaggg tattccgttt
     1021 agccgggcag agaatgatca gcaccttcgc ggcgtggggc ggcgtgagtc gctcatgttc
     1081 atcattagag gacgccagta cactgaaaat caagttcagg aaatgatggc gcgtaaaaac
     1141 cgctactact tgcaactggt tgaacaaatg accccgcaag acctggtccc tggcggcagg
     1201 gagctgctcg aacagcttcg tagcgccggt ataaaaaaag ccattgccag cggcagtaag
     1261 aactgccggc tcgtcctcga ccggcttggg atcaccgatc tcttcgacgg tatcgccgat
     1321 ggaaatagta tcacaaggag taaacctgcc cctgacgttt tcgtctgggc ggcagggcgt
     1381 gtggatgtag catgtaatgc ctgtctgggt atcgaagacg ccaatgacgg catcatagcg
     1441 atcagaactg ctggaatgcg cacgctcggt gttgggccag cagagcgatt caccccctta
     1501 cctgataagc tgcttccctc atttgcgcac gtgaaccttc aagacctgat tttctaaccc
     1561 gcattaaaat atgcgcttcc cgaaggcgct caggatcagt tcggcagcca gcatggccgt
     1621 gcggttctcc atgtccagcg tcggattgac ctctacgatg tccagcgagg tcatcttgcc
     1681 cgaatcggcg atcagttcca gcgccaggtg cgcctcgcgg tacgtcaggc cgccggccac
     1741 tttggtgccg gtgccgggcg catccctcgg gtctaccgcg tcgatatcga agctgatgtg
     1801 cagcccgttt gtaccgggcg tggcaagctc gatggcctgc tccatgatag cggccatgcc
     1861 cagacggtcg atctccttca tggtgaagac gtggatgccc acttcgcgca ggaagtcctt
     1921 ttcgccgggg tcgagatcgc gcgagccgat aatcaccacg cgatgcggat cgacctttgg
     1981 gctgatgcca cccagttcga ccagttgtgt attgccgatg ccgaccagcg ccgccagcga
     2041 catgccgtga acattgccgc tcggcgtcgt cgtatccgta ttgaaatcgg cgtgtgcgtc
     2101 gacccagatc actcccaccg gcccgcgcgc ccgcacaaca ccggtgagcg atcccatcgt
     2161 caggctatgg tctccgccga tcaccagggg aaaatcgtcc ggcccgcagc agcgctccac
     2221 cgtcgtcgcc aggctgccac acacctctaa gatggtttca acattgcgca aatgaacatt
     2281 cgcgatgtgc ggctgctcga tacgcggctg cggaatggcc aggttgccct cgtcaatcag
     2341 gggatggccg agcttgcgca gttgttcgtc gatggacgga agcagattca atcccgcgta
     2401 gcggatcgcg ttcggcccca tatccgcccc gtgtttgccc gcgcccaggt ccagcggcac
     2461 accgataaaa tgaatgttca tcgtcttttc ttcctctctt cgtcgttgaa tagcggaaac
     2521 ctgagcctat tatacctatg gctcaatggc tctgtccagg ctgatgattt catcccctaa
     2581 tctgatacac tatgtaatgg acgcaatact aaccaacgaa aggcgcacac cgtactttta
     2641 tgcaatcctt cgactggttc ccactcgtcc aacgctacac cgccgcactt gtccgcatcc
     2701 gcagcgtcag tccaggacag ggcgaaagag aggtagccca ggcggtgctg aaactgctgc
     2761 aagaggatgg tctggcatca tcctacagcg aaatcaggct tgacccgctc gtgggcgacc
     2821 cagcagggcg aagcaacgcc tatgccttcc tgcg
//
LOCUS       DASZAK010000739         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_161376, whole genome shotgun sequence.
ACCESSION   DASZAK010000739 DASZAK010000000
VERSION     DASZAK010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161376"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VGT44_12990"
     CDS             <1..833
                     /locus_tag="VGT44_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2581763.1"
                     /translation="LPGTNGSARLLAEALARAGIASLRYDKRVSGPHARENVPLLIGK
                     LNMQSHVDELAGAVRTLSNQAYVRDDRIFALANSEGTLHALSYQQHNPAIPFAGLVLT
                     GPPGRAVGTVARSQLAAQAVGIPNGEALMALYDAAIARFLAEEPIAPDPSLPEGVQMV
                     LRSLETPANLPFARELWAADAAPLLRQVAVPVLVIIGKKDIQVDWQVDGEPLQRAAAG
                     CGEVTFLFPENANHILKQELRSRSALVPAEIAESYNGPNTRLDPQTLASILEWLAAHA
                     "
     gene            complement(865..1257)
                     /locus_tag="VGT44_12995"
     CDS             complement(865..1257)
                     /locus_tag="VGT44_12995"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2581764.1"
                     /translation="MQAFAIGEVARRAGVRASTLRYYESVGLIPPPGRVHGHRRYDSS
                     IFALLAILRMAQHAGFTLAEMHMLVNGFSPETPASERWQQLARQKLVEVEELIAHAQQ
                     TKHTLESLLRCECLSLEDCVQYESAPPC"
     gene            1397..1750
                     /locus_tag="VGT44_13000"
     CDS             1397..1750
                     /locus_tag="VGT44_13000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581765.1"
                     /translation="MMNDVALIADDVPVACSLTAGERVTRAEEINDLFTGVQQVRELA
                     DGYALCFPGTSSWPQRVLDFIQGERSCCLFFTFEVAFLPNQGPIWLNIRGPEGTKEMV
                     RETLASREITLDKSS"
     gene            complement(1818..>2855)
                     /locus_tag="VGT44_13005"
     CDS             complement(1818..>2855)
                     /locus_tag="VGT44_13005"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEV2581766.1"
                     /translation="VGKVVAVLALLPPWVLIYLVAGRKGFRDGWPLAVVGSLAYIAGQ
                     LPVSQFLGPYLPDVVGALVCFAAILLLLRVWRPRTVLGYGGVELSEEEQSARDTARIG
                     SAPSGQSVLASFLPFLILIVVVVLWTGPWSPLPKIAAPSLTVKAVSSISHKVTSVVFA
                     YAPFIGGTAILASWLIMLAFIRPSLALLGQVFRDTFRQMWGALLVGFFIFGLAFVFNY
                     SGMGASLAYGFSKVGPAFILLAPILGWIGVALSGSNTSTNSLFGLVQVQVGRLLGFPV
                     LLLPTMNSVGAEVGKPVAPQTASVGVSTSKFVRDEGQVIRHNLGWTLVVLGWLMLVGL
                     LFYFFLPGLMQ"
BASE COUNT          634 a          848 c          845 g          528 t
ORIGIN      
        1 tgctgccggg aaccaatggc agcgcgcgtt tgcttgcgga ggcgctcgcg agggcaggga
       61 tcgcttcctt acgctacgac aagcgtgttt ccggacccca tgctcgcgag aatgtcccac
      121 tactgatcgg caagttgaac atgcaaagtc atgtcgacga actcgccgga gccgtccgta
      181 ccctgtcaaa ccaggcgtat gtccgcgacg accggatctt cgcgctcgcc aacagcgagg
      241 ggacccttca tgccctgagc taccaacagc acaaccccgc gattccgttt gcgggactcg
      301 tgcttaccgg ccctcctgga cgggccgtag gcacggtggc ccgatcccaa ttggctgcgc
      361 aggccgtcgg cataccaaat ggggaggcgt tgatggcgct ctacgatgca gccatcgccc
      421 gcttcctggc agaagagcct atcgcaccgg acccatcgct gccggagggc gtccagatgg
      481 tgttgcggag cctggaaact ccagccaatc tgccctttgc ccgcgagctc tgggctgccg
      541 atgcagctcc cctgctcagg caggtggcag tcccggtgct ggtcattatt gggaagaagg
      601 acatccaggt agactggcaa gtcgatggcg agccgctcca gcgggccgct gcgggatgtg
      661 gggaggtgac gttcctcttc ccagagaacg cgaatcacat cctcaagcaa gagctgcggt
      721 cccggtcagc gttggtgcca gccgagatag cggagagcta caatggcccc aatacccgcc
      781 tggacccgca gaccctggct agcattcttg agtggctggc tgcccatgcc tgatcgggtt
      841 gctgatctca actcacgaca tttatcagca aggaggagct gattcatact gaacacagtc
      901 ttctaagctc agacattcgc agcgcaacag gctctccaaa gtgtgtttgg tctgttgcgc
      961 gtgcgcgatg agttcctcaa cttccacaag cttctggcga gcaagctgct gccagcgttc
     1021 cgaggctggc gtctctggcg agaagccgtt gaccagcata tgcatctctg ccagggtaaa
     1081 gcctgcatgt tgtgccatcc gtaatatggc cagcagcgcg aagatgctcg aatcatagcg
     1141 acggtgtcca tgtacgcgac cgggaggagg gattagtccc acactttcgt aataacgtaa
     1201 tgtggatgct cgcaccccgg ctcggcgcgc gacttcacca atggcgaaag cttgcatctg
     1261 tgtttcctct caaaaatggc tcttgactta aagttcactt tatatattac actgtctttt
     1321 agacggacgc aaagggtgtc cgtggtcatg ccggaagaat ggcatagatg gttttcaaag
     1381 aagggtaaag acagttatga tgaatgatgt agcattgatt gcggacgatg tgccggtggc
     1441 ttgcagcctg acggcgggcg aacgtgtcac gcgggctgag gagatcaatg acctcttcac
     1501 cggagtgcag caggtgcgcg agctggccga tgggtatgct ctgtgttttc caggaacttc
     1561 gagctggcca caaagagtgc tggacttcat ccagggtgaa cgaagttgct gcctcttttt
     1621 cacctttgag gtggccttcc tccctaacca gggtcccatc tggctcaata tacgcgggcc
     1681 agaagggacc aaagagatgg tcagggaaac attggctagc agagaaatca ccctggataa
     1741 atcttcctga tgggatagag ggaaccctcg taggagtcgg tttaccggct cctacgactt
     1801 tcgcgtcgct tgcttgccta ttgcatcagt cccggcagga agaagtaaaa gagcagcccg
     1861 acgagcatca gccagcctag caccaccagc gtccatccca ggttatggcg aatgacctgg
     1921 ccctcgtcac gcacaaactt ggaggtcgag acgcccacac tggcggtctg gggcgcgacc
     1981 ggcttgccga cctcggcccc caccgagttc atggtgggca gcagcagcac ggggaatccc
     2041 agcagcctgc ccacctgcac ctgcaccagg ccgaacaaac tgttggtgga ggtattgctc
     2101 cctgaaagcg ctacgccgat ccatcccaga atgggggcga gcaggatgaa ggcgggaccg
     2161 accttcgaga atccgtaggc cagggacgcg cccatgccag aatagttgaa aacgaacgcc
     2221 aggccgaaga tgaagaagcc gaccagcagc gctccccaca tctggcggaa agtatcccga
     2281 aacacctgtc ccagcagtgc caggctgggg cgaataaatg ctaacatgat cagccaggaa
     2341 gccagaatgg cggttccgcc gatgaagggc gcataggcga aaacgaccga cgtgaccttg
     2401 tgactgatgg aagagactgc cttgaccgtc aagctgggcg cggcaatctt cggcagcggc
     2461 gaccaggggc cagtccagag tacgactact acaatcaata tcaagaaggg cagaaaggac
     2521 gccaggactg actgcccgga cggggcgctg ccgatcctcg cggtgtcccg cgcgctctgc
     2581 tcctcctcgc tcagttctac accaccatag cccagcacag tcctgggccg ccagaccctc
     2641 agcagcagga ggatcgctgc aaagcagacc agcgcgccga cgacgtcggg caggtaggga
     2701 ccgaggaatt gcgagaccgg caactgcccg gcaatgtagg ctaatgatcc aaccaccgcc
     2761 aacggccagc cgtcgcgaaa tcccttgcga cccgcgacca ggtagatcag cacccagggt
     2821 ggaagcagcg ccagcaccgc gacaactttc cccac
//
LOCUS       DASZAK010000740         3695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_268259, whole genome shotgun sequence.
ACCESSION   DASZAK010000740 DASZAK010000000
VERSION     DASZAK010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268259"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1897)
                     /locus_tag="VGT44_13010"
     CDS             complement(<1..1897)
                     /locus_tag="VGT44_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581767.1"
                     /translation="MRFMHIVRTRSGRYLVILFTVLIFVALLLVDVQRSYNLIPGHSS
                     PDMLRFGFSVFVAIAFLGVGTLVWLFARERWVAFLLFCFSFTVMMAFTLQTGALSGDP
                     FLSAISDSGASLSLALLTTLLLFFPMNLYAISREQAIQSVEMPHVRLRFRLIQGYLLL
                     VWGLCVLVTVQSDVSYFTQLPFPGWSLAIADVYDLIVITGTIATIIITYRRKSSLRER
                     QQLRFFVIGVVASIAPLLILTVLPQLLTAFGLPSAGYVVDSQISTTTVILLPVAFGYS
                     ILRYQILAFDRYIQRVVSWTMGVVFLAVLGYGVGVFSSLLFPSSSVFYSGVLISSLVL
                     LAPFSWWLASALSERLFFGEMRHYHQLIDKPDALVRETLDINQATELLTMAAVSAFVT
                     NEICLFVYDRESGHFQLTPALLEGDTRDAARQRLAQQLMEAIQPGGAGERRHAVTQDA
                     HWIKADQLLLRNVDTAKRPLFLSEAVKSEQEQPTGLARFISTSTEVNDPLLVAVHVQG
                     EMIGLLALGERGDHGQYAGPDFEAIDLLLTRYSPLLENARLYEQANRHGAMLNALYGA
                     NVSLQRSYQTINEVAIAYATLAAGTARAGADIWLVSGSDGAIEYIDHVGEEPRLPAVN
                     TLQENDWR"
     gene            complement(1921..3024)
                     /locus_tag="VGT44_13015"
     CDS             complement(1921..3024)
                     /locus_tag="VGT44_13015"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV2581768.1"
                     /translation="MSDDAQAPANLFAAHQAFLRERLQTILATLHPALRADVERALAE
                     KGKLLAHPRADASTLSPALPAGTWPLLTLLVAQHIAPDIDLTRAGKVALAVECFVCAL
                     DLLDDVEDEDQTPVVQELGTGRILNVSTALLALSQLSLLSLRQQKVPPALILRLLWAF
                     DECTIGAVTGQHRDLLAEQRPAREFTREECIEIAAGKAGAIMRLACQSGAICAGASKK
                     VMQQFAELGTTLGIAHQLDNDAHDLYHLLHHDFSPALDSGISSGQPKSDLARGKKTLP
                     IVLSALSLADQPGREALANTFDADLKNFTELSDKIKEEYRDILNEGILATWGICLLYR
                     ERARERLQEIEVQRPVALELRLLLGFESSSGGT"
BASE COUNT          962 a          975 c         1018 g          740 t
ORIGIN      
        1 cgcgccaatc gttctcttgc agtgtgttga ctgcaggcag gcgcggttcc tcgccaacgt
       61 ggtcgatata ctcgatcgct ccgtcgctcc cggagacgag ccagatgtcc gcgccagccc
      121 tggcagtgcc ggctgctaga gttgcatagg caatggcgac ctcgttgatc gtctgatagg
      181 agcgctgcag cgacacattc gcgccgtaga gcgcattcag catcgctcca tgccggttgg
      241 cctgctcgta caggcgcgca ttttccagca gcggggagta acgtgtcagg agcaggtcga
      301 tggcctcaaa atcagggccg gcgtattgac cgtgatcgcc acgctcgcca agcgcgagca
      361 ggccgatcat ttcgccctgg acatgcacgg caaccagcag tggatcattg acttccgtcg
      421 aggtcgaaat gaagcgcgcg aggccggtcg gctgctcttg ctccgatttg acggcttcac
      481 tgaggaacag ggggcgtttt gccgtgtcaa cgttgcgcag cagcaactga tctgctttga
      541 tccagtgcgc gtcctgggtc acggcgtggc gccgttctcc tgcgccgcct ggctggatcg
      601 cctccatcaa ctgctgggcc aggcgttggc gggcggcgtc gcgcgtatca ccttcaagca
      661 aagcaggggt aagttgaaag tggccgcttt cccggtcgta cacaaacagg catatttcgt
      721 ttgttacaaa agcgctgacg gcggccatcg tgagcagctc cgtagcctgg ttgatgtcga
      781 gcgtttcgcg gaccagcgca tctggcttgt cgatcaactg gtggtaatgc cgcatctcac
      841 caaagaagag acgttcagat aaggcgctgg ccagccacca ggagaatgga gccagcaaga
      901 ccagcgaaga aatcaggact cccgaataga aaacagacga tgaagggaac agcagactac
      961 taaaaacgcc cactccatag ccaagcactg ccaggaagac tacccccatc gtccatgata
     1021 ctactcgctg aatatagcgg tcgaaggcga gaatctgata gcgcaggatg gaataaccaa
     1081 aggcaaccgg caaaaggatg acggtagtcg tgctgatctg gctatcaact acatagcctg
     1141 cggagggtaa gccgaaggca gtcagcaatt gcggtaggac ggtcaagatc agcaatggag
     1201 cgattgatgc aactaccccg atcacaaaaa agcgcaactg ctgtcgttcg cgcaatgatg
     1261 attttcggcg gtatgtgatg attatagttg caatggtgcc tgttataaca atcaagtcgt
     1321 atacatctgc aatggcaagc gaccagccag gaaaaggtaa ttgagtgaaa taggacacat
     1381 cggattgcac cgtcaccagg acacacaatc cccatactag cagcaaataa ccctggatta
     1441 gcctgaagcg caggcgaacg tgaggcatct caacggattg tatggcctgt tcgcgtgaaa
     1501 tggcataaag gttcataggg aaaaaaagta acaatgtcgt aagcaaggcc agagataacg
     1561 aggcgccgga atcactgatg gctgatagga atggatcacc cgatagcgcg cctgtttgca
     1621 gcgtgaatgc catcatcacc gtgaaagaaa aacagaagag gagaaaggcc acccagcgct
     1681 cgcgcgcgaa tagccagacg agtgtgccaa cacccagaaa ggctatggcg acaaacacag
     1741 aaaaaccaaa gcgcagcatg tcgggtgagg agtgaccggg tatcaggtta taggaacgct
     1801 ggacatcgac gagcagcaaa gcaacaaaga tcaataccgt gaagagaata acgaggtaac
     1861 gtcccgacct ggtacgcact atgtgcataa accgcatgaa aagctcctcc atcctgcacg
     1921 ttacgtacct cctgaagatg actcgaaccc caggagcaga cgcaattcca aagcaacagg
     1981 ccgctgaact tctatttctt gcaaacgctc acgagcgcgt tcgcgataga gcaagcaaat
     2041 accccaggtc gccaggattc cttcattcag tatatcccga tactcttcct taattttatc
     2101 ggataactct gtgaagttct tcaaatcagc atcaaaggtg ttcgctagcg cttctcttcc
     2161 aggctgatct gccaggctca aggcggatag tacgatggga agggtcttct ttcctctcgc
     2221 caggtcactt ttcggttggc cgcttgaaat tcctgaatcc agggcgggag aaaaatcatg
     2281 atggagtaaa tgatacaggt cgtgcgcatc attgtcaagt tgatgagcaa tgccgagcgt
     2341 tgtaccgagt tcggcaaact gctgcatgac tttcttgctt gctcccgcac agattgcgcc
     2401 tgattgacag gcaagacgca taatagcgcc cgccttacct gcggcgatct cgatacactc
     2461 ctcgcgcgtg aattcacgcg cgggtcgctg ctccgccagc aggtcccggt gctggccggt
     2521 aacagcacca atcgtacact cgtcaaaagc ccacaacagg cgcaggatca aagcaggagg
     2581 aaccttctgc tgccgtaaag ataggagaga aagttgcgat agcgccagga gcgccgtcga
     2641 gacgttcaat atgcgccccg ttcccaattc ctggacaaca ggtgtttgat cttcatcctc
     2701 gacatcatcg agcaagtcga gcgcgcaaac aaaacactca accgccaggg caactttgcc
     2761 ggcccttgtc aggtcgatat ccggcgcgat atgctgcgca accagcaacg tcaaaagtgg
     2821 ccaggtcccg gctgggagag ccggggaaag ggtagaagca tcggcgcggg ggtgagcgag
     2881 caacttcccc ttttcggcga gcgctcgctc gacatcggct cgcagcgccg ggtgcagggt
     2941 ggccaaaata gtttgaagcc gctcacgtag gaatgcctgg tgagcggcaa acagattggc
     3001 aggagcctga gcgtcatcac tcatacaata cccatagcgg aggcagcatc gactctctct
     3061 acactatgcc ccttcgtatt ttatgcccct gcttattgaa actactatta acagtgttgc
     3121 caccagcaga gagacttatc caccggcggc tgctcgaggg tcagttgcgg cactagtcgc
     3181 gacacaaccc ggctcacgcg tggcgagaag ccctcgcgca taatgacacc ctccgagtcc
     3241 ttgcgcagct cttcacggag ttctgaatcg gttgagacgc gatgtacgaa cgattgaagc
     3301 tggagttctt ccatgggggg aaacgttcct ttcctttttg ttccaataga tcgttggaaa
     3361 cggttttcgg accataccat tcgacaagtt ggcacagatc agcaaagcaa gtgatcggca
     3421 actacgggga tcatgatata cgaatgggaa tatacagggt gcgcgctcag caggaaatac
     3481 gtattatacg agctatcacc gctacaaccc gatggttgtg taacaaagta tacatcaaaa
     3541 atatggaaaa tgcaagagct tattttaaag tctattttgg atggaaaaac gatagatata
     3601 caaagcaaga gcatggaggg tgagcaaggc actgtcggcg aaatgaaaag tagtgacgga
     3661 agggcgacca caagggtgcg cccttaccat gactc
//
LOCUS       DASZAK010000741         5460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_410375, whole genome shotgun sequence.
ACCESSION   DASZAK010000741 DASZAK010000000
VERSION     DASZAK010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410375"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VGT44_13020"
     CDS             <1..344
                     /locus_tag="VGT44_13020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HEV2581769.1"
                     /translation="RKGTDMYKVTVLYGQPTNPAAFEQHYYGTHLPLCAKMQGIKGFT
                     IGKLGALEPGQQSPYYRVASLYVDSPAALHATLTSPEGQAVVGDIANFATGGVTLMVS
                     EEEVLVPIAIK"
     gene            complement(337..2214)
                     /locus_tag="VGT44_13025"
     CDS             complement(337..2214)
                     /locus_tag="VGT44_13025"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HEV2581770.1"
                     /translation="MPTNYIANRAPLQPTPFIALPPGAIEPGGWLRDQLHIQAEGLTG
                     HLDEFWPDVGPQSGWLGGSGESWERGPYYLDGLLPLAYLLKDEALIAKTQPWIEWTLA
                     SQGADGQFGPRANEDWWSRMIMLKVLMQYEEASGDERIVPFLTRYFRYQSAHLPSHPL
                     SGWSQARGSENILAVQWLYNRTGEPFLLDLIELLHMQSFDWTDIFTHFPFWRPQTSNY
                     DLRAHVVNVAMALKEPALYGLLSGKALHRDAARRGIESLLLYHGQVNGIFSGDEMLAG
                     THPSQGTELCAVVEYMFTLEHLVRVFGDGYYADLLERVAFNALPATISEDWHSHQYDQ
                     QVNQVACTLARRNWTANGDESNLFGLEPNYGCCTANMHQGWPKFAAHLWMATDDGGLA
                     AVSYAPCSVTALVADGVKVTLDVETKYPFDGQISIAVYPERPARFPIKLRIPGWGAPD
                     IKIGSEIITATNREDGYFSIEREWEDGDSITLNFDMQARIERRGQGAVGILAGPLVFA
                     LPIKERWVKLREKGLYGDWEVHPDSPWNYGLMIDQNDLESSFELQKQPMTRQPFIATD
                     VPLRLMARGQRISSWDVAMNSADTPPLNPELSGEPVEEITLVPYGSAKLRIAEFPVML
                     T"
     gene            2397..3536
                     /locus_tag="VGT44_13030"
     CDS             2397..3536
                     /locus_tag="VGT44_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581771.1"
                     /translation="MKNRRILFLLAPGMLAGIVIAILALMPAAAQASGGSFIGGLHVN
                     VIGSTVPHNGDVNPYGIVTAPTTTGDLTQGNILISNFNAVSNLQGTGTTVVQLTPGGS
                     LSVFTHLKAGELNGNCPGGVGLTTALVALQRGWVIVGSLPTSDGTSATAKAGCLIVLN
                     NWGQPVETFSGSPINGPWDMTAFDQGNEASLFVTNVLNGTVDAKGSVVNKGTVVRIDL
                     NVPWPGHGNPAIEDTTVIGSGFSERTDPAALVIGPTGVGLSGNTLYVNDSVNNRIAAI
                     PNAIERETSALTGKDVTSNGELNDPLGLTIAPNGDILTVNGNDGKIVETTPGGTQIAH
                     KLIDKTITPPSTVPGAGCLFGLTIIPGGAGVYFVDDCTNTLNTLS"
     gene            3687..4259
                     /locus_tag="VGT44_13035"
     CDS             3687..4259
                     /locus_tag="VGT44_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEV2581772.1"
                     /translation="MTIPADHTPGPPQGLWIYDDYATLPNDGHRYEIVDGVLYMAPAP
                     NEWHQTATGRLFRYLASYIEDAGHGRVYIAPFDVELGPGSVLQPDVLVILNAHRERIT
                     FSRIVGAPDLVVEVVSPGSVGYDRTKKQNAYARAGVPEYWIADPWSRTIEVLTLEADG
                     YRSLGVFEGKAKLPSEIVPDFPVQVERFFA"
     gene            complement(4268..5341)
                     /locus_tag="VGT44_13040"
     CDS             complement(4268..5341)
                     /locus_tag="VGT44_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEV2581773.1"
                     /translation="MSHTTIRSIAVEPLDILLLEPFTIATGSVSAARNVLISVTLEDG
                     SVGYGECAPFPPSTGETQETALAAARGCAALIEGGNAAHWRVLSRLMHSVYFSQHTAI
                     AGMEMALLDALTRSYGIPMYVFFGGASSAIETDYSIPMVTPEHGYELAQEVVRRGITT
                     IKVKVGGDIREDVARVEAIREGAPGLGLTLDANQGYSANQALLCLEALDDRDIRPLMM
                     EQPVHKDDYDGLRYVTQHTTVPIAADESVSKAADATRLIAMGACNVINIKLMKCGFVE
                     ALDIAAVCRANHVELMIGAMIESRLATSAAAHFVAGLGGFRYIDLDTPMLLADDPFSG
                     GYEQRGGVYDVSGVGKGLGVEQR"
BASE COUNT         1118 a         1674 c         1512 g         1156 t
ORIGIN      
        1 atagaaaggg taccgatatg tacaaggtta cagttcttta tggccagcca acaaaccctg
       61 ccgcatttga gcagcactac tacggaacgc atctccccct ttgcgcaaag atgcagggca
      121 tcaaaggatt caccatcggc aagttaggcg cgctcgaacc ggggcaacaa agtccttatt
      181 acagagtcgc cagcctgtat gttgacagcc cggcagcctt acacgccact ctgacttctc
      241 ctgaaggaca ggcggttgtg ggcgatatcg ccaacttcgc cacaggaggc gtcaccctca
      301 tggtcagcga agaagaggtg ctggtgccga tcgcgatcaa gtaagcatca ccgggaattc
      361 cgcgatacgc agtttcgcgc tgccatacgg cacgagcgtg atctcctcta ccggctcgcc
      421 gctcagctcc gggttgagcg gcggcgtatc agccgagttc atcgccacat cccacgacga
      481 gatacgctgc cctcttgcca tcagcctgag tgggacatcg gttgctatga aaggctgtcg
      541 cgtcatcggc tgcttttgaa gctcaaacga gctttcgagg tcgttttgat cgatcatcag
      601 cccatagttc cagggcgaat ctggatgcac ctcccaatct ccataaagac ccttttcgcg
      661 caattttacc cagcgttcct tgatgggcag ggcaaagaca agcggcccgg ccagtatgcc
      721 aaccgcgcct tgtccgcgcc gctcgattcg ggcttgcatg tcaaagttta gcgtgatgct
      781 gtccccatct tcccattcac gctcaatgga aaaatatcca tcctcgcggt tcgttgctgt
      841 gatgatctca ctccctatct tgatatccgg cgcgccccag cctgggatgc gtagcttgat
      901 ggggaagcga gccggtcgct ccgggtaaac agcgatggag atttgcccat cgaaagggta
      961 cttcgtctca acgtcgagcg tcaccttcac gccatcggcc accagcgcag taacactgca
     1021 cggagcatac gagacggcgg ccagcccgcc atcgtccgtc gccatccaca ggtgcgcggc
     1081 gaatttgggc cagccctggt gcatgttagc ggtgcagcag ccgtagttgg gttccaggcc
     1141 gaacaggttc gactcgtcgc cattggccgt ccagttgcgc ctcgccagcg tacatgccac
     1201 ctggttgacc tgctggtcgt actggtggct atgccagtcc tccgaaatcg tggcgggcag
     1261 cgcgttgaag gccactcgtt caagcagatc ggcgtagtat ccatctccaa aaaccctcac
     1321 caggtgttcc aacgtaaaca tatattcgac gacggcgcag agttccgttc cctggctggg
     1381 atgcgttccc gccagcatct catcgccgga gaagatgccg ttgacctgcc cgtgatagag
     1441 caacaggctt tcgatgcccc tgcgagccgc gtcgcggtgc agcgccttcc ccgatagaag
     1501 cccatacagc gctggttcct tcaaagccat cgccacgttg acgacgtggg cgcgcaaatc
     1561 atagttgctc gtctgcggtc gccagaaggg gaaatgggtg aaaatatccg tccaatcgaa
     1621 actttgcata tgcagcagtt cgatcaggtc gagcaggaat ggctcacctg tgcggttata
     1681 cagccactgc acggcgagta tgttttcgct gccgcgcgcc tggctccatc cggaaagcgg
     1741 gtgcgagggc aggtgcgcag actggtagcg gaaatatcgt gtgagaaacg gcacgatgcg
     1801 ttcatcgccg ctcgcctctt cgtattgcat cagcaccttg agcataatca tgcgcgacca
     1861 ccagtcctcg ttcgcgcgcg gcccaaactg cccatcggct ccctggctgg cgagcgtcca
     1921 ttcgatccag ggctgcgttt tggcgatcag cgcttcatcc ttgagcaggt acgccagggg
     1981 cagcagcccg tccaggtagt acggcccgcg ttcccagctt tcgccactgc cgccgagcca
     2041 gccgctctgc ggcccgacat ccggccagaa ctcgtcaagg tggcccgtca atccttccgc
     2101 ctggatgtgc aactggtcgc gcagccagcc ccccggctcg attgcgccgg gcggcaaagc
     2161 gatgaacggg gttggctgta acggcgcgcg gttggcgatg taatttgtgg gcatgagata
     2221 tctatccctc tctacaaccg atattgataa ttgttgtccg caacctacac cgaaaattta
     2281 tcaaatcgaa tgcctcaatg taacctcata tcgtatatct taatcggggg gaaaactttt
     2341 ttccaatctt ccaatctttc cgctttattg tacatctaaa actctggagg tatctcatga
     2401 agaatcggcg tattctcttt ttgctcgcgc cggggatgct ggctgggatt gttattgcga
     2461 tcctggcatt aatgccggcg gcggcacagg cttcgggtgg ttcgtttatc ggtggccttc
     2521 atgtgaatgt tatcggctcg acggtccccc acaacggcga tgtcaaccca tatggcatcg
     2581 tgaccgctcc cactacaact ggcgatctga cacagggcaa tatccttatt agcaacttta
     2641 acgcggtatc gaatttgcaa ggaaccggca cgaccgtcgt gcagttgacg ccgggcggat
     2701 cattaagtgt atttacgcac ctcaaagctg gcgagctgaa tggcaactgc cccggcggcg
     2761 ttggtttgac gacggcgctg gttgcgctgc aacgcggctg ggtgatcgtc ggcagcctgc
     2821 cgaccagcga tggcacgtcg gccaccgcca aggccgggtg cctgatcgtt ttgaacaact
     2881 gggggcagcc cgtagaaacc ttctccggct cgcccatcaa cggcccctgg gatatgactg
     2941 cctttgatca gggcaacgaa gccagcctct tcgtcaccaa cgtcctcaac ggcacagtcg
     3001 atgccaaggg cagcgtcgta aacaaaggca cggtcgtgcg cattgatctg aacgttccct
     3061 ggccgggaca cggcaatcct gcgatcgagg acaccaccgt catcggctca ggcttctcgg
     3121 agcgcaccga tcccgccgcg ctggtaatcg gcccgacggg agttggcttg agtggcaata
     3181 cgctctacgt caacgatagc gtgaacaatc gcatcgccgc tatccccaac gccatcgagc
     3241 gcgaaacttc cgcactgacc ggcaaagatg tcacctccaa cggcgaactg aacgaccccc
     3301 tgggtctgac catcgccccc aacggcgata ttctgacggt taacggcaac gacggcaaga
     3361 tcgtcgaaac aacgccgggc ggcacgcaga ttgcgcataa gctgatcgat aagactatca
     3421 ctccgccctc cacggttccc ggtgcgggct gtctcttcgg cctgaccatt atcccaggcg
     3481 gcgctggcgt ctacttcgtc gatgactgta ccaatacgct gaataccctc agctagagga
     3541 gcgcccccct ctcttctctt caggaaggac gccccttgcg ggcgtccttc tcttatgtta
     3601 ctatgggaat gtagtgatgg acgacatcta taacttgtag gagaggcaca gcaacaatga
     3661 ccatgacagc atctgggaga gtgccaatga caatccccgc cgaccatacc cccggccctc
     3721 cccagggact atggatctat gacgactacg caactcttcc caatgacggg catcgctacg
     3781 aaattgtgga tggagtattg tatatggcac ccgcaccgaa tgagtggcat cagacagcca
     3841 ctggccgcct attccgttat cttgcgagct acattgaaga cgctggtcat ggaagggttt
     3901 atattgcccc ctttgatgta gagttaggcc ccggaagcgt tttacagccg gatgtcctcg
     3961 tcattctaaa tgcacaccgt gaaagaatta cgttctctcg cattgtggga gcgccagatc
     4021 tggttgttga ggtagtctca ccgggctcag tgggttatga ccgtaccaaa aagcaaaatg
     4081 cctacgcccg cgccggagtg cctgaatact ggatagctga tccctggtca cgcacgattg
     4141 aggtcctcac tttggaagcg gacggctatc gctcgctcgg tgtctttgag ggcaaggcaa
     4201 agcttccatc agagatcgta ccagatttcc ccgtacaggt tgagcggttc ttcgcgtaac
     4261 agcgcattca gcgctgttca actcccagcc cttttcctac acccgatacg tcgtacaccc
     4321 cgccgcgctg ctcgtaaccg ccgctgaacg gatcatcggc cagcagcatc ggcgtatcca
     4381 ggtctatgta gcggaaccca cccaggccgg cgacgaaatg cgcggcagcg ctggtggcca
     4441 gccgcgactc tatcatcgcg ccgatcatca gttccacgtg attcgcgcgg cacacggcgg
     4501 cgatgtccag cgcttcaaca aagccgcact tcatcagctt gatattgatc acattgcacg
     4561 cgcccatcgc gatcaggcgc gtggcatcgg cagccttcga gacgctttca tcggcggcaa
     4621 ttggcaccgt cgtgtgctgt gtgacgtagc gcaggccgtc gtaatcgtct ttatgcaccg
     4681 gctgctccat catcaggggg cggatgtcgc ggtcgtccag cgcttccagg cagagcagcg
     4741 cttggttcgc gctgtagccc tgattggcgt caagcgtcag ccccaggccc ggcgcgccct
     4801 cgcggatggc ctcgacgcgc gccacatcct cgcggatatc gccgccaact ttgaccttga
     4861 tcgtcgtgat gccgcgcctc accacctcct gagccagctc gtagccatgt tcgggcgtca
     4921 ccatcggaat gctgtaatcg gtctcaatcg cgctgctcgc cccgccgaag aacacataca
     4981 tgggaatgcc atacgagcgc gtcagcgcgt caagcagcgc catctccatg cccgcgatag
     5041 ccgtatgctg gctaaaatag acgctgtgca tcagtcgcga aaggacgcgc cagtgcgcag
     5101 cattcccgcc ctcgatcagg gccgcgcagc cgcgcgcagc cgccagcgcc gtctcttgcg
     5161 tttcgcctgt gctgggcgga aacggcgcgc attcgccata ccccacgctg ccatcctcta
     5221 acgttacact aatcagcacg ttgcgcgcag cagagacgct gcccgtcgca atggtgaatg
     5281 gttccagcaa aagaatgtcg agcggctcaa ccgcgataga acgaatagtg gtatgtgaca
     5341 tttattcctc ctcttcttcg tgttcttcgt gcgtagggac agggggtaga ggtggagagg
     5401 agtgggggcc ttgtgcctgt cctggtggag gtcacgatgc gctttgccac caggacaggc
//
LOCUS       DASZAK010000742         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_338095, whole genome shotgun sequence.
ACCESSION   DASZAK010000742 DASZAK010000000
VERSION     DASZAK010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338095"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VGT44_13045"
     CDS             <1..249
                     /locus_tag="VGT44_13045"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2581774.1"
                     /translation="SDTGIGISAEHLPHLGERFYRVDKARSREAGGTGLGLSIARSIA
                     ATHDGELTLTSVAEQGTTVTVKLPLAQVIHSDLAMEEV"
     gene            362..1369
                     /locus_tag="VGT44_13050"
     CDS             362..1369
                     /locus_tag="VGT44_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2581775.1"
                     /translation="MIHFQDDDDKGTPSPYAYWNEGDAAYYPSTSSYTSSAWPQSQVT
                     PQPLQQDEQPTDPQLPVVPIEAQPPSPPKGKRGALRTGILAAMMLLIVLIFGTGLFAG
                     WQFSRASSTTGGGASATVAQTAATSTVQTAQEAVIAKVKPAVVQINVTTASGSAIGSG
                     VIIDTRGYIVTNNHVVANAQSIEVVLNDGTHLTAQLVGSDSADDLAVLKITTTKQLTA
                     ISLGDSTKLQVGQDVLAIGNPLGITDTVTSGIISALNRTVSEGQGAATISNAIQTDAP
                     INPGNSGGALIDLQGNLIGIPTLGAIDTEFNTPANGVGFAIPSNLVAKVSQEIIATGN
                     V"
     gene            1462..1578
                     /gene="rrf"
                     /locus_tag="VGT44_13055"
     rRNA            1462..1578
                     /gene="rrf"
                     /locus_tag="VGT44_13055"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(1628..>2144)
                     /locus_tag="VGT44_13060"
     CDS             complement(1628..>2144)
                     /locus_tag="VGT44_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581776.1"
                     /translation="QVIGRLKRYRIVAKMAALVMSIGALLIALMTPATGIVLAISILV
                     LTGIGIGPFFTLPMLVVQNSLPADRLGISTAGLRYLGQLGASLGIAIVGTVVTSSVSG
                     NMMNHLPTNATSKLALSAALQHGFFAVLIFALIALVSTFLLKEAQIVGTQETFPQEQA
                     VEESDKELVTA"
BASE COUNT          505 a          604 c          609 g          426 t
ORIGIN      
        1 agtgatacgg ggatcggtat cagcgcggag catctccccc atctgggtga gcgcttttac
       61 cgcgttgata aggcgcgttc gcgcgaagcc ggaggaacgg gcctcggcct ctcgatcgct
      121 cgcagtatcg ctgctactca cgatggcgaa ctgacgctga ccagcgtcgc cgagcaggga
      181 acgaccgtca cggtcaaact gcccctggcg caggttatcc attctgatct tgcgatggaa
      241 gaagtatgag aaactcctca ttttcttcca atatgcttca aatctttatg cggtaagcta
      301 ttgatacaag atgatctcga taccatcttt gtgtaaccat ctacaatcag aggtataaac
      361 gatgatacat tttcaagatg atgacgacaa aggaacgcct tctccctatg cgtattggaa
      421 cgaaggcgat gcggcctact atccatctac ttcctcttac acttcgtctg cgtggccaca
      481 aagccaggtg acgccacaac cgttgcagca agacgaacag ccaacggacc cgcaattacc
      541 cgttgtgccg attgaggcac aacccccctc tcctccaaag ggaaagcggg gtgcgctgcg
      601 caccggtata ttggcggcga tgatgttgct gatagtcctg atcttcggca ccggcctgtt
      661 cgcgggctgg caatttagcc gcgcgagcag tacaaccgga ggtggtgcga gcgcgacggt
      721 cgcgcaaacc gctgcgacga gcacggttca gacggcgcaa gaggcagtca ttgcgaaggt
      781 caagccagct gtcgtgcaga tcaacgtcac aacggccagc ggaagcgcga tcggctctgg
      841 tgtgattatc gatacacgcg gctatatcgt gaccaacaac catgtggttg ccaatgcaca
      901 gagcattgag gtcgtgctga atgatggcac ccatctcacc gcgcagctgg tgggaagcga
      961 ttccgcggat gacctcgccg tgttgaaaat cacgacgacg aagcaactga cggctatcag
     1021 cctgggtgac tctacgaagc tgcaggtggg gcaggatgtg ctggcgattg gcaacccgct
     1081 gggcatcacc gatacggtga catcgggcat catcagcgcg ctgaaccgca cggtctccga
     1141 gggtcagggg gccgcgacga tctcgaacgc catccagacc gacgccccga ttaacccggg
     1201 caatagcggc ggcgcgctaa tcgacctgca gggcaacctg atcggcattc ccacgctggg
     1261 cgcgatcgac accgagttca atactcctgc caatggcgtc ggcttcgcca ttccctcgaa
     1321 cctcgtcgct aaggtgtccc aggaaattat cgcgaccgga aatgtttagg cccaaaaaaa
     1381 ggctgaaaaa actattgaca gggcatacga actgtagtat catatgccct gtcaaaacga
     1441 accgcctgta tgcgcaggtg aaccggtgac aatagcgaag agggtacacc cgttcccatc
     1501 ccgaacacgg aagttaagct cttcagctcc gatgatactt gaggggcagc cctccgggaa
     1561 aataggacgt cgccgtttca cccgctcaca cgggcggttt tgttatgcct tcaaggttgt
     1621 cgaagaccta agcagtgacc agttctttgt cggattcctc aactgcttgc tcctgtggaa
     1681 atgtctcctg cgtcccaact atttgcgcct ctttcaggag aaaggtagac accagagcga
     1741 tcaaagcaaa gatcagtacg gcgaaaaagc cgtgctggag cgcagcggac agtgctaatt
     1801 tggaagtcgc gttcgttggc agatgattca tcatgttgcc agagacagag ctggtgacga
     1861 ctgttcccac aattgcgatg cccagagagg cccccagctg tcccaggtag cgcagcccgg
     1921 ctgtgctgat gcccaggcgg tcggcgggca gcgaattctg gaccaccagc atcgggaggg
     1981 tgaagaaggg gccaatgccg atgccggtca gcaccagaat cgagatggcg agtacgatgc
     2041 cggtcgctgg agtcataagc gcgatcagca atgcgccaat gctcataacc agcgccgcca
     2101 ttttggcaac gatacggtag cgcttcagtc tgccgataac ctgc
//
LOCUS       DASZAK010000743         1686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_124624, whole genome shotgun sequence.
ACCESSION   DASZAK010000743 DASZAK010000000
VERSION     DASZAK010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124624"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /gene="mutM"
                     /locus_tag="VGT44_13065"
     CDS             <1..952
                     /gene="mutM"
                     /locus_tag="VGT44_13065"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEV2581777.1"
                     /translation="WRRAIAAMPELPEVEHTARQLRETIIGVAIASAQVFWERTISRP
                     DLSEFLAEMEGRRIVGVRRRGKLLIIDLSGDALLTIHRRMTGNLLLLPPGWEIDSSLR
                     ERDPAAWSTRGPAFYHERQDAVEIDPAHLRYCRVCFTFSDGRRLLYTDPRKFGRIALW
                     PRACEAQALEGYGIEPLGDEFTVEELTHSLAGHAGAIKPLLLNQAVIAGIGNIYADEA
                     LFSASIHPLRPASSLTQEELRSLHDAIVAVLALGIEHGGTSFNEYRDLWGEAGENYDH
                     MLVYQRDGKACPRCGTPIERIVVAQRGTHFCPHCQKIIGA"
     gene            complement(939..1415)
                     /locus_tag="VGT44_13070"
     CDS             complement(939..1415)
                     /locus_tag="VGT44_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581778.1"
                     /translation="MKNKHYTLIGLLLPIAFLLGACTGFGAASTSQVAPQQTVVISKT
                     FQSQLSPLPTVPTYLCGAWASNNAPGPNDTITIFARLTKNLVAVSGATATAVVHFQSG
                     DVTLNTNPVSDNGGYVTFSLSLQGRQPANIPVTVDVTFTHFAGGTLLCTQAFFTPQ"
BASE COUNT          381 a          456 c          520 g          329 t
ORIGIN      
        1 ctggaggaga gcaatagcag ctatgcccga actgcctgaa gtagaacaca ccgcgcgaca
       61 attgcgcgaa actatcatcg gcgtggccat cgccagcgcc caggtcttct gggaacgcac
      121 catcagccgt cccgacctgt ccgaattcct ggcagagatg gaaggacggc gcatcgtcgg
      181 tgtgcgcagg cgcgggaaat tgctcatcat cgacttgagc ggcgacgcgc tactgaccat
      241 ccaccgccgc atgacgggga acttgctgct gctcccgccc ggctgggaga tcgactcgag
      301 cctgcgagaa cgagaccctg cggcatggag tacacgcgga ccggcgtttt accacgaaag
      361 acaggatgcc gttgaaattg acccggcaca cctgcgctat tgccgcgtgt gctttacctt
      421 ctcggatggg cgcaggctgt tgtataccga tccgcgcaag tttgggcgca tcgcgttgtg
      481 gccgcgcgcg tgtgaggcgc aggcgttgga ggggtatggc atcgagccgt tgggggatga
      541 gtttacggtg gaggaattga cgcacagcct ggcgggacac gcgggcgcga tcaagccgct
      601 gctgctgaac caggcagtca tcgcgggcat tggcaatatt tatgccgacg aagccctgtt
      661 ttctgcctcg atccatccgc tgcgtcccgc ctcgtcgctt acccaggaag agctgcggtc
      721 gctgcatgat gctatagtcg cggtgctggc gctgggcatc gaacatggcg gtacgtcgtt
      781 taacgaatat cgcgatttgt ggggcgaagc aggcgagaac tacgaccaca tgctggtcta
      841 ccagagggac ggcaaagctt gcccacgatg cggcacaccg atcgagcgca tagtggtggc
      901 gcaacgtggg acgcatttct gcccgcattg ccagaaaatt attggggcgt gaagaacgcc
      961 tgcgtacaga gtagcgtgcc accggcgaaa tgcgtgaaag ttacatcaac cgttactgga
     1021 atattggcag gctggcgacc ttgtagggag aggctaaagg tcacatatcc cccgttatcg
     1081 ctgaccgggt tagtattcag cgtgacatcc ccactctgaa aatggacgac agccgtcgcc
     1141 gtcgcgccac tcactgcaac gagatttttc gtcaggcggg caaaaatggt gatggtatca
     1201 ttcgggccgg gcgcgttatt cgatgcccac gcgccacaca gataggttgg caccgtcggc
     1261 aaaggtgaaa gttgcgattg aaacgtcttg ctgatgacta ccgtttgttg cggcgcaacc
     1321 tggctcgtgg aggcagcccc aaagcctgta caggcgccaa gcagaaacgc aatcggcaac
     1381 agcagcccga tgagcgtgta gtgtttgttc ttcataagcg gtgtctatcc agcggatact
     1441 tcaacaaaac agaccaaagt acgtgtagat tatagcatag acaatcaacc gaccgtaggc
     1501 gccgatttac tgtgcgccta cgatttgtac gctgctcagc aatacacaga catttggaga
     1561 taaggaatta ggtgggagag tcaggagcag acggcttagc taaggcgggc aggagactct
     1621 cctgcggggc agaacccagc acgtcgagca attcttctag ggagaagatg ccatagcagc
     1681 cagcga
//
LOCUS       DASZAK010000744         4014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_161446, whole genome shotgun sequence.
ACCESSION   DASZAK010000744 DASZAK010000000
VERSION     DASZAK010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4014
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161446"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VGT44_13075"
     CDS             <1..831
                     /locus_tag="VGT44_13075"
                     /inference="COORDINATES: protein motif:HMM:NF013070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV2581779.1"
                     /translation="WIASGEHDAPAYRYASQQAQRVILSICLSGFGSASKIAELIEEH
                     IPGLRNQDVEILCMDISLSSKTTEEVLQLVRNRQVVAVVGTINPHLEDYPFISLTDFL
                     FRDGIMRLRTLLGETLIDPALLNPAFAASSIPQDRPGEEQHAPAFLQRADLVRQITAT
                     LNQQMIFLNPVRVMPLIERMIELIEAEVGEAFDLDVLAGLILHLACILERGTLPNGML
                     VNEAVRSQVGQLFPRELSICRRALHILSEQIARTIPDEEAYNIVGILRQVDIFIVEAR
                     "
     gene            1185..1517
                     /locus_tag="VGT44_13080"
     CDS             1185..1517
                     /locus_tag="VGT44_13080"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV2581780.1"
                     /translation="MIRVLIVCSWGMSTSLLVEGMLAAAKQKNIVLTIEALSAGEYAA
                     KVDECDVVLIAPQIRHLRKSIEKLAQSAGKPVGLIEPFFYATMNGPAVLEQVLVLFAT
                     ATRGAKEA"
     gene            1514..2845
                     /locus_tag="VGT44_13085"
     CDS             1514..2845
                     /locus_tag="VGT44_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV2581781.1"
                     /translation="MSAMIERSSEWVERYLVPPLGAIAGNIYLQAVRDAFIIFTLPLI
                     LAGSLFLIIANPPIPALATLVAPYQNAILVPFELSFGLMALFLSFGVAYSLAQYRRTE
                     AVQPGVLAMVLFLIASVPVGNISNLTLGTILPYLGGNGLVVAILIGILTTEVVRRFRG
                     SRFTIRLPKSVPPNVTRAFEALVPALFLITLVWGLEWFVSSFTTGSGSSVQQLTLPIL
                     LMRAFEPLVYVQDSYPAALLEIVLMMLLWSVGIHGMNVVTAIALPFWLSVLTTNATAH
                     GHGIVTEPFFHIFAHLGGSGATWPLVIYMLRSRSAQLRTVGKVALGPAIFNINEPVTF
                     GVPMALNPLMMIPFVLVPVIIVTINYIVFAVGMVHVPVVLQPFTVPVGISGFVATGGD
                     IRGSLLQVFDLAVSAVLYYPFFKAWERILVQREEAATKQEESRPLVQAVIR"
     gene            3032..>4014
                     /locus_tag="VGT44_13090"
     CDS             3032..>4014
                     /locus_tag="VGT44_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2581782.1"
                     /translation="MKLFVGKRKALASILGTLFLSFVIVACGSANTGGGTTPTANGNG
                     GKGCKKIGVSLPETATSNRWDGFDRPLLQQLIPQMLTGATVDYANAEGNADTQQTQAD
                     AMLTKGDCILVVAANDSIKAARIVADAKAQGVPVIAYDRLIQSNDLAYYVSFDNVQVG
                     KLQGTYISNNYKSFVKSGVGITVMIDGAQTDNNALLFAQGAHSILDPLFSSGVLKNVY
                     EKFTPNWDNPTAQTEMEAALTANGNKIQIAYVANDGMATTVIAALKAQHLNGKVLVTG
                     QDATLTGLQNILLGNQSMTVLKPYKLEAGATASLVAAISNGSSTSSLVNGQT"
BASE COUNT          870 a         1149 c         1113 g          882 t
ORIGIN      
        1 tggattgctt ctggcgaaca tgatgcgcca gcctaccgtt atgcctcaca gcaagctcag
       61 cgtgtcattc tctccatctg cctgtccggt tttggcagcg caagcaagat tgcggaactg
      121 atcgaagagc atataccagg actgcgtaac caggatgttg aaatactgtg catggatatc
      181 agcctttcga gtaaaaccac cgaagaagtc ctccaactag tgagaaaccg gcaggtcgtc
      241 gctgtcgttg gcacgataaa cccgcatctc gaagactatc ccttcatctc cctgaccgat
      301 ttcctcttcc gcgacggcat tatgcgcctg cgcaccctgc tcggcgaaac attgatcgac
      361 ccggcgctgc ttaacccggc gttcgcagct tccagtatcc cacaggatag gccaggcgag
      421 gaacaacatg ctccggcatt cttgcaacgc gccgacctcg tacgccagat caccgcaacg
      481 ctcaaccagc aaatgatctt cctcaatccg gtacgggtca tgccgttgat cgagcgcatg
      541 atcgaactga tcgaggccga agtgggcgag gcattcgacc tggacgtact ggcgggcctg
      601 atcttgcacc tggcctgcat cctcgaacga ggcacacttc caaacgggat gctcgtcaac
      661 gaggccgtgc gatcgcaggt cggacagctc ttcccgcgag aattgagcat ttgccgacgc
      721 gccctccaca ttctgagcga acaaatcgcg cgcactatcc ccgacgaaga agcctacaac
      781 atcgtcggca tactgaggca ggtcgatatc ttcatcgtcg aagcccgcta agccgactac
      841 ccccggtcgt tgggacgcga tgatgaatct taacgaccaa gatcggccct gtcatcctga
      901 gcgcagcgaa ggatctcggt cctggcgcag gagatccttc gctgcgctca ggatgacaga
      961 cgatgattac cgatttggtt ggtaaaaatc ataatcgcat ccccctgtta gtgtgtagca
     1021 cactatagca cacattctgg cattaaactt gcgtatactt gagtataccc ataagagtgt
     1081 tgtcatcagg cattcaatat gcgagccgcc ctggtcggct tgtggtatgt tgataattgc
     1141 gaccacctga acacagactt cacgctatgc tgggggaatc acagatgatt cgggtactta
     1201 ttgtatgcag ttggggcatg tctaccagct tgctggtcga aggtatgtta gcagcagcga
     1261 agcagaaaaa catcgtgttg accatcgagg cgctcagcgc gggcgagtat gccgcaaaag
     1321 tggatgaatg cgacgtggtg ctgatcgcac cgcagatcag gcatctgcgc aagagcatcg
     1381 agaagctggc gcaatctgcg ggtaagccgg tggggttaat cgagccattt ttctacgcga
     1441 ccatgaatgg tccggcagtc ctggaacagg tccttgttct gttcgcaacc gcaacgaggg
     1501 gggcgaagga agcatgagcg cgatgatcga gcgcagcagc gaatgggtcg agcgctatct
     1561 agtgccgcca ctcggcgcga tagccggcaa tatctatttg caggcggtgc gcgatgcctt
     1621 cattattttc accttgcctc tcatcctggc aggcagcctg ttcctgatca tcgccaaccc
     1681 gcctattccg gcgctggcga cgttggtcgc gccctatcag aacgccatcc tggttccctt
     1741 tgagctgtcg tttggcttga tggcgctctt cctctcgttc ggcgtcgcct acagcctggc
     1801 gcagtaccgg cgcaccgagg cagtccaacc gggcgttctg gcgatggtac tgtttctgat
     1861 cgccagcgta cccgtcggca atatctccaa tctgacactt ggcacgatcc tgccctatct
     1921 gggaggcaac ggcctggtgg tggctatcct gataggtatt ttgaccactg aggttgtgcg
     1981 gcgtttccgc ggctcgcgct ttacgatcag gctgccgaaa agtgtgccgc cgaacgtgac
     2041 gcgcgctttc gaggcactcg ttccggcgct ctttttaatc acgttggtct ggggactcga
     2101 atggttcgtc agctcattca cgaccggctc aggcagctca gtgcagcaac tgaccttgcc
     2161 aattttattg atgcgcgcct tcgagccgct cgtgtatgtg caggatagct acccggcggc
     2221 gctgctcgag atcgttctga tgatgctgct ctggtcggtc ggaattcacg gcatgaacgt
     2281 cgtgactgcc atcgcgctgc cgttctggtt gagcgtcctg accaccaacg ccacggcgca
     2341 tgggcatggt atcgtgaccg aaccgttctt tcacatattt gcccacctcg gcggttcagg
     2401 cgcaacctgg cccctggtga tttatatgct gcgttcgcgt tcggcgcaat tgcgcacagt
     2461 gggtaaggtt gcgctggggc cggccatctt caacatcaac gagccggtga cattcggtgt
     2521 accgatggcg ctcaacccgt tgatgatgat cccgttcgtg ctggtgcctg tcatcatcgt
     2581 caccatcaac tacattgttt ttgcggttgg catggtgcat gtgccggtgg tcctgcaacc
     2641 gtttactgtt cccgtgggaa ttagcggttt cgtagccaca ggaggcgaca ttcgtggcag
     2701 tctcctacag gtctttgacc tggccgtttc cgctgttcta tattacccgt tcttcaaagc
     2761 ctgggagcgc atccttgttc aacgcgagga agctgcgaca aagcaagaag agagtcggcc
     2821 cctcgttcag gctgtaatac gctagtctgg tgacagcgcg tatcgagccg gttcggtggc
     2881 acagaaagga ggaagagacc gtctcaagta gaaaaggtac acgtcgtact agcagtttgt
     2941 tcaagtttag tcttacattg tacacctttc aaagcgaaaa gctgacagtt tcagcgaacg
     3001 ggaaaggtgc aacagggagg acaggattac gatgaaacta tttgttggga agcgcaaggc
     3061 gcttgcctcc atcctcggaa ctctttttct cagttttgtg attgtcgcct gcggcagcgc
     3121 caataccggc ggcggtacta cacccaccgc caacggcaac ggcggcaaag ggtgcaagaa
     3181 aatcggcgtc tccttgccag aaaccgccac ctccaatcgc tgggatggct ttgaccgacc
     3241 gctgctccaa cagctcatcc cacagatgct taccggcgcg acggttgatt atgccaatgc
     3301 cgaaggaaat gccgatacgc agcagacgca agctgatgca atgctgacca agggtgactg
     3361 catcctggta gtggccgcca atgatagcat caaagcggcc cgcatcgtag ccgacgcgaa
     3421 agctcagggc gtgccggtga tcgcttatga ccgcctgatc cagtcgaacg acctggccta
     3481 ctacgtctca ttcgacaacg tacaggtcgg taagctgcaa ggcacgtaca tctccaataa
     3541 ctacaagtcg ttcgttaaat cgggcgtcgg cataacggtc atgatcgacg gcgcgcagac
     3601 cgacaacaac gcgctgctct tcgctcaggg cgcgcactct attctcgacc cgctgttcag
     3661 tagcggcgtc ctcaagaacg tgtacgagaa gttcacgccc aactgggaca acccgaccgc
     3721 gcagacggag atggaagcag cgctgacggc gaacggaaat aagatccaga tcgcctacgt
     3781 tgcgaacgat ggcatggcca ccaccgtcat cgccgcgctc aaagcgcagc acctcaacgg
     3841 caaagtgctg gtcaccggcc aggatgcaac ccttacggga cttcagaaca ttctgttggg
     3901 caaccagtcg atgacagtct taaagccata taaacttgag gctggtgcga cggcgtcgct
     3961 ggtggcggcc atcagcaatg gcagcagcac cagctctctg gtcaatggcc agac
//
LOCUS       DASZAK010000745         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_268393, whole genome shotgun sequence.
ACCESSION   DASZAK010000745 DASZAK010000000
VERSION     DASZAK010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268393"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..989
                     /locus_tag="VGT44_13095"
     CDS             <1..989
                     /locus_tag="VGT44_13095"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEV2581783.1"
                     /translation="IFGGGNKLEVRLADARIGSFVCSICRSTTEQGLDGTLRGMKAFF
                     TQPICDAAKHMAGIWGRNFPAQLSQILALPQNVNSSAAVRYVYDEYQRLRDVLAVQAG
                     HEITDDAIHASLLVYNENRRLQRELYRIKRDDPARLSMIETYLLIRAGTRIRREEHNA
                     LLQEALRLLPERRVRPRDKPRVVFLGGFCEQPPLEMIETIEDACFIVDDDLLIGQRWI
                     TQDVPMEESDPVWALAESYVERSASSPVQYDARKPKEEELMRMLKEARAEAAIITAAK
                     FCEPGLDDQLALSHKLDAEKVPYLVLEFEEKMTSFEQMSMQVETFAESLLFH"
     gene            1002..2297
                     /locus_tag="VGT44_13100"
     CDS             1002..2297
                     /locus_tag="VGT44_13100"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HEV2581784.1"
                     /translation="MNVTNTKTGIPDARAAGQRLVNQWLANLGTARERGQPVAYVFVM
                     GNAIEILRTFGFELAFPEINSLQSGVRKSAPDFISKAEDFGFSPDVCSYVKADVGLML
                     SNMMHPSGIQMPKPDIAIASSLCNVYIKWAEIWERRFNTPAFVLDIPGKRFAAWKSAA
                     GSPQHLADARWVEGQFRDLITRCEQITGKKFDIDRLAEVEENVNEMIRLWIDILAFNQ
                     NRPAPYNSMLDGLTYMGVLNAWRGTPEGVEYMHLLHDEVAGKVARGEGRVPNERFRLV
                     FSGTPCYVNMRRLIELFESHGGVFAASDYLTYAAGGLDSAGLFYDTSRPLESLAEVTT
                     LASQMGLSNHFFSHHALAQQMKDFAADGIVYHGIKSCRTVATSMADNREYVTTRHGMP
                     TLYIESDLIDPRYWSDAQIKNRVDAYFEVLEQRARLEGR"
     gene            2299..3105
                     /locus_tag="VGT44_13105"
     CDS             2299..3105
                     /locus_tag="VGT44_13105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEV2581785.1"
                     /translation="MNYVAGIDVGSTQAKGIIMDEYAEIVGKAILDMETNMGTAAERV
                     FERVLANANLSPSDIAFTVATGYGRYRVTFGDLQVTEISCHARGAVAEFPKTRTVLDI
                     GGQDTKAIRIGDSGEVTDFAMNDKCAAGTGRFLGAASFALEMPLSDLGPLALKSTRPV
                     KITTTCTVFAETEIIGHLAKGHLPEDVLMGMHQSIATRCASLVRRVGMASEVTFSGGV
                     SRNVAMVALLEEVLGTPLNVSSKSQFMGAIGAGLYALDNLQARRHTHLAS"
     gene            3172..>3737
                     /locus_tag="VGT44_13110"
     CDS             3172..>3737
                     /locus_tag="VGT44_13110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HEV2581786.1"
                     /translation="MILAVGIDIGSTYTKAVMMDDQKQVRATAIRRSGYKPASAAAAV
                     FDDLVANAGVTKEQVTYIATSGYGRYMVPFRHTQITELTCHALAIAYHFPQVRTILDI
                     GGQDIKAIRIDERGRVRAFRLNDKCAAGTGAFLEKTARYLGLTTGDIGLYALRSTKPV
                     EVSSVCAVFAESEVINHLSIGIAPEDIM"
BASE COUNT          847 a         1078 c         1018 g          794 t
ORIGIN      
        1 ccatatttgg cggcggcaac aagctcgaag tgcgcctggc cgatgcgcgc atcggctcct
       61 tcgtctgctc gatttgccgt agcacgaccg agcaaggcct cgacggaacc ttgcgcggta
      121 tgaaagcctt tttcacacag cccatctgtg atgctgccaa gcacatggcc ggtatctggg
      181 gccgcaactt tcccgctcag ttgtcgcaaa tactggccct tcctcagaat gtaaattcat
      241 ccgcagcggt acgttacgta tacgacgaat accagcgatt gcgcgatgtc ctcgccgtcc
      301 aggcgggcca cgagatcacc gatgatgcca tccatgccag cttgctcgtc tacaacgaga
      361 atcgccggct gcaacgcgaa ctgtatcgta tcaagcgtga cgaccccgcg cgactttcca
      421 tgatagaaac ctatctactc attcgtgctg gtacgcgcat ccgccgcgaa gagcataatg
      481 cactgctgca agaggccctg cgactcctgc cggagcgccg cgtgcgcccg cgtgacaagc
      541 cgagagtcgt attcctggga ggcttttgcg agcaaccgcc gcttgagatg atcgaaacca
      601 ttgaagatgc ctgtttcatc gtcgatgacg atctgctgat cggtcagcgt tggataactc
      661 aggatgttcc aatggaagag agcgatcctg tgtgggccct cgctgagagc tatgtcgagc
      721 gttccgcgtc aagcccggtg cagtatgatg cacgcaaacc aaaggaagaa gaattgatgc
      781 gcatgctcaa ggaagcgcga gcggaagcag ccatcattac cgccgccaaa ttttgcgaac
      841 ctggcctcga cgaccagcta gcgctctcac ataagctaga cgccgagaaa gtgccatacc
      901 tcgtgctcga gttcgaagaa aagatgacca gttttgaaca aatgtcaatg caggtggaaa
      961 ccttcgccga atcattgctg ttccattgat gggaggtcac tatgaatgtc accaacacga
     1021 aaaccgggat accagatgcg cgcgcagcgg gtcaacgcct ggtaaaccag tggctggcaa
     1081 acctcggcac cgcgcgcgaa cgcggtcaac ccgtagcata tgtattcgtc atgggcaatg
     1141 ctatagagat tctgcgcacg ttcggatttg agctggcctt cccggaaata aattccttac
     1201 agagcggcgt gcgcaaatcc gcaccggatt ttatctccaa agccgaggac tttggcttct
     1261 ccccggatgt ctgcagctac gtcaaagccg acgtcgggct gatgctcagt aatatgatgc
     1321 atccttccgg catacaaatg ccgaaacctg atatcgccat cgcgtcgagc ctgtgcaacg
     1381 tctacatcaa atgggccgaa atctgggagc ggcgtttcaa cactcctgcg ttcgtcctgg
     1441 acattcccgg taagcgcttt gccgcgtgga aatcggcagc cggtagcccc cagcacctgg
     1501 ccgatgcgcg ctgggtagag ggccagttcc gcgatctcat cacacgctgc gagcagatca
     1561 ccggcaaaaa atttgatatt gatcgccttg ctgaggttga ggagaatgtg aacgagatga
     1621 tccgtctctg gatcgatata cttgcgttca atcaaaaccg gcctgctccc tataactcga
     1681 tgctcgatgg cctcacctac atgggcgtgc tcaatgcctg gagaggaact cccgaaggcg
     1741 tcgagtacat gcacctgctc catgatgagg ttgctggcaa ggttgcgcga ggagaaggcc
     1801 gcgtgcccaa cgaacgtttc cgcctggtct ttagtggcac tccctgttat gtgaacatgc
     1861 gtcgcctgat agaactcttc gagagccacg ggggggtgtt cgccgcttct gactacctga
     1921 cctatgcagc aggtggcctc gatagcgcgg gactcttcta cgacacctca cgccctctcg
     1981 agagccttgc cgaagtcaca acgctcgcct cgcaaatggg cctctcgaat cacttcttct
     2041 ctcatcatgc cctggcgcag cagatgaagg atttcgcggc ggacggcatc gtctatcacg
     2101 gcatcaagag ttgtcgcacc gtagcaacca gcatggcgga caaccgcgaa tacgtcacca
     2161 ccaggcacgg catgcccacg ctctacatcg aatcggatct gatcgatcca cgttactggt
     2221 ccgacgcgca aatcaaaaac cgcgttgacg cctacttcga agtccttgaa caacgggcaa
     2281 gattggaggg ccgctaaaat gaactatgta gcaggtatcg acgtcggatc aacacaagca
     2341 aagggtatca tcatggatga atatgccgaa atcgttggca aggccatcct tgatatggaa
     2401 accaacatgg gcacagccgc tgagagggtt ttcgagcgtg tacttgccaa tgcaaacctg
     2461 agccccagcg atatcgcttt caccgtcgcg actggctatg gtcgctatcg tgtcaccttc
     2521 ggcgatctgc aagtgaccga gatcagttgc cacgcgcggg gcgccgtagc tgagttccca
     2581 aagacgcgca cggtgctgga tatcggtggg caagatacca aagccattcg catcggcgat
     2641 tcaggggaag tgacggactt cgccatgaat gacaagtgcg cagcaggaac ggggcgcttc
     2701 ctgggcgcag cctcatttgc gctcgaaatg cccctatctg atctagggcc attagcgctg
     2761 aaatcaaccc ggcccgtcaa gattaccacc acctgtaccg tcttcgcaga aaccgagatc
     2821 atcggtcacc ttgcgaaggg acatcttcct gaagatgttc tgatgggtat gcatcagtct
     2881 attgccaccc gctgcgcctc gcttgtccgc cgcgtcggca tggcttcaga agtcacattc
     2941 agcgggggcg tctcgcgtaa tgtcgctatg gtggcgcttc tggaagaagt tcttggcaca
     3001 cccttaaatg tgagttcgaa gtcacaattc atgggcgcaa tcggtgccgg gctgtacgcg
     3061 ctcgacaacc tacaagcccg tcgccatact catctggctt cgtaggggcg acctttatgg
     3121 tcgcccgttc ttgcccggat gcgctcatcc gcagggtaag gaaggtgtac gatgattctc
     3181 gcggttggga tcgatattgg atccacctac accaaggccg ttatgatgga cgaccaaaag
     3241 caggtacgcg ctaccgctat tcgtcgtagt ggttacaaac cggcgtcagc agcagcggca
     3301 gtgttcgatg accttgtagc gaatgctggt gtcacaaaag agcaggtgac ctatatcgcc
     3361 acctctggtt acggccgcta catggtgcct tttcgtcaca ctcagattac cgagctgacc
     3421 tgccacgcgt tggcaatcgc atatcatttc ccgcaagtgc gtaccattct ggacataggc
     3481 ggacaagaca tcaaagctat ccgtatcgat gagcgcgggc gagtgcgtgc tttccgcctc
     3541 aacgataagt gcgcggccgg tacaggcgca ttcctggaga agacagcccg ttacctcggt
     3601 ctcacaacag gcgatatcgg gctatacgcg ctccgctcta cgaagccagt cgaagtcagt
     3661 agtgtctgcg ctgtcttcgc ggaatccgag gtgatcaacc atctctcgat cggcattgcc
     3721 cccgaggaca tcatgca
//
LOCUS       DASZAK010000746         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_517120, whole genome shotgun sequence.
ACCESSION   DASZAK010000746 DASZAK010000000
VERSION     DASZAK010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517120"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /locus_tag="VGT44_13115"
     CDS             <1..905
                     /locus_tag="VGT44_13115"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2581787.1"
                     /translation="TTYQILTYRKKREKNNGHERDKDQEPSMDDYPHFTLFGSYDWGL
                     IIYDEVHLLPAPVFRITAEIQARRRLGLTATLVREDGREADVFSLIGPKKYDVPWREL
                     ERQGWIATAECHEIRVSMTEDDQLNYAISEEREKYRLAAENPAKLDVVRHLVAKHRDD
                     QVLIIGQYLDQLDLLAKELNAPMMNGKTPTKQREKLYEQFRQGEIKHLVVSKVANFAI
                     DLPDANVAIQVSGTFGSRQEEAQRLGRILRPKSDGSIAHFYTVVTRDTRDQDFSANRQ
                     LFLTEQGYRYLIDDAEEVLASPAV"
     gene            1015..1368
                     /locus_tag="VGT44_13120"
     CDS             1015..1368
                     /locus_tag="VGT44_13120"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV2581788.1"
                     /translation="MAEQDENYKNITSRDFPAQVLQAEQMVVVNFSSGKSGACQIFDP
                     EFMAASKEYKGRVTFARLNLDDEKDETLVNEWRIDGIPTVIFYKQGQEVNRIKGIVMR
                     EKLRKQIEGVLLAHF"
     gene            1397..>2053
                     /locus_tag="VGT44_13125"
     CDS             1397..>2053
                     /locus_tag="VGT44_13125"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HEV2581789.1"
                     /translation="MHPIKLASSLHETIWGGRRFERDGWKQLPPGGAPIGEAWETEIN
                     TVAQNQPYRGKTLGALVEEFGAALLGQQAITAFGRRFPLLAKFIDANAKLSVQVHPDD
                     AYAAQHEGGKLGKTECWYILAAAPGASIVHGFKENTSREAVRRAIESVALESLLEEVP
                     VAPGDVIFVPAGTVHAIGSGVMLYELQEYSDITYRMYDYGRLTAAGTPRELHIAQSLA
                     V"
BASE COUNT          494 a          614 c          575 g          370 t
ORIGIN      
        1 tgacgaccta ccagattttg acctaccgca aaaagcgcga gaaaaacaac ggccacgagc
       61 gcgacaagga ccaggaaccg agcatggacg actaccccca cttcacgctc ttcggcagct
      121 acgactgggg cctcatcatc tatgacgagg ttcacctgtt acccgcgccg gtcttccgca
      181 tcaccgccga aatacaggcg cgccgccgcc tcgggctaac ggccacgctg gtgcgcgagg
      241 atggacgcga ggccgacgta ttcagcctga tcgggccgaa gaagtacgat gttccctggc
      301 gcgaactgga acgacagggc tggatcgcaa cggcggaatg ccacgagatc cgagtgagca
      361 tgaccgagga cgatcagctc aactacgcca tctccgaaga gcgcgaaaag tatcgcctgg
      421 cggctgagaa ccccgccaag ctggatgtcg tgcgccacct ggtcgcaaag catcgcgacg
      481 accaggtact catcatcggc cagtacctcg accagctcga cttgctcgcc aaagaactca
      541 acgcgcccat gatgaacggc aaaacgccga cgaagcagcg cgaaaagctc tacgaacagt
      601 tccgccaggg cgagatcaag cacctggtgg tcagcaaagt ggccaacttc gccattgatc
      661 tgccagacgc gaacgtggcc atccaggtat ccggcacctt cggctcgcgc caggaagagg
      721 cgcagcgcct gggtcgcatc ctgcgcccaa aatcagacgg cagtatcgct catttctata
      781 ccgtcgtcac acgcgataca cgcgaccagg acttttccgc gaaccgccag cttttcctga
      841 cggaacaggg ctatcggtac ctgatcgatg atgccgagga ggtacttgcc tcgccagctg
      901 tttaaccagt tttgcttgac atcgcctttt gcgggtgata ctcttcaagc acgaacttag
      961 catagaaatc ggccatctcg tatagcagtg catataggcc gttaagagga tagtatggca
     1021 gagcaagacg aaaactacaa gaatatcacc agccgggact ttcctgcgca ggttctccag
     1081 gcagaacaga tggtcgtcgt gaatttttca tccggcaaga gcggcgcctg ccagatcttc
     1141 gatcccgaat ttatggcggc cagtaaagaa tacaaggggc gcgtgacgtt tgcccggctg
     1201 aatctcgacg atgagaaaga cgaaacgctc gtcaatgagt ggcgtattga tggcattcct
     1261 accgtgatct tttataaaca gggccaggaa gtcaatcgca taaaggggat tgttatgcgc
     1321 gagaagctgc gcaagcagat cgaaggcgta ctactcgcgc acttctgatg aacatgtaga
     1381 agggtttgtg tggttaatgc atccgatcaa attagcatcc tcacttcacg aaacgatctg
     1441 gggagggaga cgttttgagc gcgatgggtg gaaacaactc ccgcccggag gcgcgcccat
     1501 cggcgaagcc tgggaaaccg aaatcaacac cgtcgcgcag aaccagccat accggggcaa
     1561 aacgctcggc gcgctggttg aagagttcgg agcagcgctg ctcggccagc aggccataac
     1621 ggccttcggt cggcgctttc ccctgctggc caagttcatc gacgccaacg cgaaattgtc
     1681 cgtccaggtt caccccgacg atgcctatgc cgcgcagcac gaaggcggca aactgggcaa
     1741 aacggaatgc tggtacatcc tcgctgccgc ccccggcgct tcgattgtgc atggcttcaa
     1801 agagaacacc tcgcgcgagg ccgtgagacg agcaatcgag tctgtcgcgc tcgaaagcct
     1861 gctcgaagag gtgccggtcg cacccggcga tgttatcttc gtaccggccg gaaccgtcca
     1921 tgccatcggc agcggcgtga tgctctacga actccaggaa tactctgaca tcacctaccg
     1981 catgtacgac tacggtcgcc tgactgcagc cgggacgccc cgtgaactac atatcgcaca
     2041 atccctggcc gtc
//
LOCUS       DASZAK010000747         1923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_410612, whole genome shotgun sequence.
ACCESSION   DASZAK010000747 DASZAK010000000
VERSION     DASZAK010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410612"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..592
                     /locus_tag="VGT44_13130"
     CDS             41..592
                     /locus_tag="VGT44_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581790.1"
                     /translation="MPTEKQLLSYFLELLPQCKSSRTSCEEGKGKVSRKPDKGEDVCF
                     FNFNKENQAVACLALKIQQNAIKKGERVICDCLIYFGRRDSKRDAYEETFCLVELKGS
                     DLKHAMRQLINTYDKLWRLLEDSSCKGQLQLFTKKAYIYKHGNAPKETKDKDFVALRR
                     KLEARFDDFAHHRDSDIGTFLRK"
     gene            complement(597..1583)
                     /locus_tag="VGT44_13135"
     CDS             complement(597..1583)
                     /locus_tag="VGT44_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581791.1"
                     /translation="MLDQNDLLPEEQSNPALIEELRTTYRMRPEEQQALARVHERLAN
                     SSHPLPPVDAVRIDDLARARQRATRVALPTRNAGFRRKWLTRLNAFAAVIIVVLLIGS
                     LALTFSLINHTRVGSPAGNDIRVVLEPVNGSNPSQAEMQAASVILSQRFSSFGLSGAS
                     ALVVTLHGLLAIQVDLPHFGGNEQQITATLVEPGNLEFWCTGPFPLPTGKTFDPSQFY
                     AQYNPGDQPRFTGKDLDPNQIYVSHDQAGRPQINFEMGGSAIARFGTFTANNIGEYLT
                     ITFDRKVIESAVIQSAITGPGVLNGNFTEQQANAIVAVLKAGSLPVGLREVG"
     gene            complement(1577..>1923)
                     /locus_tag="VGT44_13140"
     CDS             complement(1577..>1923)
                     /locus_tag="VGT44_13140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2581792.1"
                     /translation="HNKLLNVYRQASRHPRVALDTVADSLFEEDGPEQHALRQEERGQ
                     LRAALQRLPEMQQHILQLRYGDGLRCTEIALLLDKREEAVRKLLSRSILILRQVYNIN
                     QVNRQTEGESSC"
BASE COUNT          467 a          492 c          524 g          440 t
ORIGIN      
        1 atcggagata ttttttgagt tgtaagtgag gacatcagga atgcccactg aaaagcagtt
       61 gttgagttat tttttggagt tgttgccaca gtgtaaatcg tccaggacaa gctgtgaaga
      121 aggcaaagga aaagtcagca gaaaaccgga taaaggggaa gatgtatgct ttttcaactt
      181 caataaagag aatcaagccg ttgcatgtct ggcactgaag atacaacaaa acgcaatcaa
      241 aaaaggcgaa agagtcattt gcgactgcct gatttatttt ggcagacgag actcaaaacg
      301 cgatgcatat gaagaaacct tctgtctggt tgagttaaaa gggagcgacc taaaacatgc
      361 catgaggcag cttataaata cttatgacaa gctttggcgc ctgctagagg atagttcttg
      421 taaaggtcaa cttcaactgt tcactaaaaa agcctacatc tacaaacatg gtaatgctcc
      481 aaaggagacg aaagataaag atttcgtagc gctcagaaga aagctagaag ccagattcga
      541 tgactttgcg catcaccggg atagtgatat cggtacattc cttcgcaaat aaggaatcat
      601 cccacctccc tcaacccaac cggcagcgac cccgccttca gcaccgcgac aatcgcgtta
      661 gcctgctgct ccgtaaagtt gccgttaagc acgccggggc cggtgatggc gctctggatc
      721 accgccgact cgattacctt ccggtcgaac gtaattgtca ggtattcgcc gatattgttg
      781 gcggtgaatg tcccaaacct ggcgatagcg ctgcccccca tctcgaagtt gatttgcggg
      841 cgaccggcct ggtcatggct cacatagatc tggtttgggt cgaggtcttt gcccgtgaac
      901 cggggctgat cgccaggatt gtactgtgcg taaaactggg acgggtcgaa agtttttccg
      961 gtaggcaatg ggaagggacc cgtacaccag aattccaggt tacccggctc caccagcgtc
     1021 gcggtaatct gctgctcgtt gccgccgaaa tgcggcaggt caacctggat ggcgagcaag
     1081 ccgtgaagcg tcactaccag cgcgcttgcc ccgctcaaac cgaaactgct gaaacgctgt
     1141 gagagaatca cgcttgcagc ctgcatttcg gcctgagaag gattgctgcc attgaccggc
     1201 tccaacacta ctcgaatatc gttaccggca ggcgaaccta ccctggtgtg attgatgaga
     1261 gaaaaggtca gcgccagcga gccaatcagc agcacaacga taatgaccgc cgcgaatgcg
     1321 ttgagccgtg tcagccactt gcgcctgaaa cctgcattcc gggtaggaag tgcaacccga
     1381 gtggcgcgtt gccgagctcg cgcaagatca tcaatccgaa cagcatcgac cggcgggagt
     1441 ggatgcgaac tgttggcgag acgctcgtga acgcgcgcca gcgcctgctg ttcttcaggt
     1501 ctcatgcgat aagttgttcg tagttcctcg atcaaggcag gattgctctg ttcttcggga
     1561 agtaaatcat tctgatctag catgagcttt ctccttcggt ttgccggttg acctggttga
     1621 tattgtaaac ctggcgcagg atcaggatgc tgcgtgagag cagtttgcgc actgcctcct
     1681 cgcgcttgtc taggagcagg gcaatttcgg tgcagcgcag cccatcgccg tagcgtaact
     1741 gcaagatgtg ttgttgcatc tccggcagcc tctggagtgc ggctcgtaac tgccctcgct
     1801 cctcttgccg gagcgcgtgt tgttctggcc catcttcctc gaacaggcta tccgcaaccg
     1861 tatccaacgc gacgcgcggg tgtctgctcg cctgtcggta gacattgagg agcttgttat
     1921 gcg
//
LOCUS       DASZAK010000748         3262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_302506, whole genome shotgun sequence.
ACCESSION   DASZAK010000748 DASZAK010000000
VERSION     DASZAK010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302506"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1274
                     /locus_tag="VGT44_13145"
     CDS             120..1274
                     /locus_tag="VGT44_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEV2581793.1"
                     /translation="MSIQSFNIAIPQTSIDDLRERLARTRWPDSAEGAGWNSGTSLAY
                     MRELVHYWQHGYDWRIQETALNQFAQFKTEVDGQGIHFLYERGKGPNPIPLLLIHGWP
                     DSFYRFHKVIPMLTDPERFGGDPQTSFDIVVPSLPGFGFSDRPGEGGMKSIRSADLLA
                     RLMHERLGYKQFAAAGGDIGSRVARLMALAYPEWLIGIHLTDIGFPREVAFPPDVPNP
                     SPVEQRFLGSVQGWFFQEGAYAMLQTTKPQTISYPLNDSPVGLAAWIVEKFRAWSDCD
                     GDIEKSYTKDELLTNCMIYWVTETISSSARLYREDGLQSAPQLKPGQYIEVPAGVATF
                     PKDLTVPPRELGERFLRVKHWREMPRGGHFAALEEPELLVGDLQAFFRQV"
     gene            1408..2445
                     /locus_tag="VGT44_13150"
     CDS             1408..2445
                     /locus_tag="VGT44_13150"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2581794.1"
                     /translation="MERYGYRFSFGPWNIHEGADPFGPPVRAVRDFESKLEVFKELGF
                     DAVQLHDDDAVPGLDDLAPEQLRQQAQAMHHRLRDAGLMAEFVAPRLWEHPMTIDGAF
                     TSNDAQAREYALERSKKAIDIGHELGTNLLVLWLAREGTYLREAKDARLATQRLVDAL
                     DALLDYDPHVRIAIEPKPNEPMDLAYVPTMGHGIALGAQTKEPRRMGVLMESAHAILA
                     GLDPSDEIAFALAFDKLWSVHLNDQNGLKYDQDRSFGSVDLRRAFNQVRVLDNNHYWQ
                     FGIVGLDVKAMRTQPAELATKHLRNSLHVFLRLVEIVRSIDQHRIDALIAARDYEELD
                     WVIFNYLTGRS"
     gene            2465..>3262
                     /locus_tag="VGT44_13155"
     CDS             2465..>3262
                     /locus_tag="VGT44_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2581795.1"
                     /translation="MPAFAHRVEPFNSTIFTEINLLAQQYNALNLGQGKPDWETPPDI
                     VEQLARAVQAQQYNQYAPGPGTLTLRRAVADHAARFYDMDIDAGRGVVVTAGATEGIF
                     CAVMGLVDPGDEVIMIEPFYDSYPASIMMAGATPVCVPLRPPDWRLDPDELRAAFTKK
                     TRALILNTPHNPTGRVFSRQELGMIADLCIEHDVVVISDEVYEHLLYDDAEHVPIATL
                     PGMFERTVTVSSAGKLFSATGWKIGWVYGYPDLVDGVARAHQFITFAV"
BASE COUNT          688 a          884 c          986 g          704 t
ORIGIN      
        1 acagaggagc atagaggcag agagaagcac catctccctg cttggaaggg tccttcagaa
       61 catgagagag ttgcgacttg tctcacatca agtcacagag tgcaaaggag aaatgcttca
      121 tgagtatcca atctttcaac attgctattc cgcagacctc cattgatgac ctgcgcgaac
      181 gcttagcccg cacccgctgg ccagattcgg cggagggagc aggctggaac tctggcacaa
      241 gccttgcgta tatgagagag cttgttcact actggcagca cggttacgac tggcgaattc
      301 aggaaactgc gctcaatcag tttgcgcagt tcaaaaccga ggtcgatggg cagggcattc
      361 attttctcta tgaacgtggg aaaggaccca atcccatacc tttgctcttg attcatggct
      421 ggcctgactc gttttaccgc ttccataagg tcattcccat gcttaccgac ccagaacgct
      481 tcggtggaga ccctcagacc tcctttgaca tcgtcgttcc cagccttccg ggcttcgggt
      541 tctctgatcg tccgggagag ggagggatga agtcgatccg ctccgctgac ttgttggcgc
      601 gcttgatgca tgagaggctt ggttacaagc agtttgccgc cgctggtggg gacattggga
      661 gccgtgttgc acgcctgatg gctttggcct atccagagtg gctcatcggc atccacctga
      721 ccgacattgg tttcccacga gaagtcgcct ttcctcccga tgtacctaat ccctcacctg
      781 ttgagcagcg gtttcttggc tccgtccagg gatggttctt ccaggaagga gcctatgcca
      841 tgcttcaaac aacaaagcca caaaccatct cctacccgct caacgactcg cccgtcggct
      901 tagctgcgtg gatcgttgag aagttccgtg catggagcga ctgcgatggc gatatcgaga
      961 agagctatac caaagacgag ctgctcacca attgtatgat ctactgggtg acggagacga
     1021 tttcttcttc ggctcgtctc taccgtgagg atggactcca atcagcgcct cagctcaaac
     1081 ctgggcagta tattgaagtc ccagcagggg ttgccacgtt tcccaaagat ctgacggtgc
     1141 cgccgcgtga actgggcgag cgcttcttgc gcgtcaagca ctggagagaa atgccccgag
     1201 gaggacactt cgccgcgctg gaggaacctg aattgctggt tggggacttg caggcattct
     1261 tccgtcaagt gtagaaagat aggaggatga cgatttgcta attgtgatcg atgcccactc
     1321 gaatccctgg tatgcatcgg gttgagatgc tatcattatg ccataccctg gttcatatga
     1381 tgcatcgctc gcgaaggaga cccgccgatg gaaaggtatg gctatcgctt ctcatttggc
     1441 ccctggaata ttcacgaggg ggcagacccg tttgggccac cggtgcgggc tgtgcgcgac
     1501 ttcgagagca agctggaggt gttcaaggaa ctgggcttcg acgcggtaca gttgcatgac
     1561 gatgatgccg tgccggggct ggatgatctt gcaccggagc aactgcggca gcaggcgcag
     1621 gcgatgcatc acaggctgcg ggatgccgga ctgatggccg agtttgtcgc gccgcgcctg
     1681 tgggagcatc cgatgacgat agacggcgcg ttcacctcca atgatgcgca ggcgcgcgag
     1741 tacgcgctgg aacgctccaa gaaagctatt gatatcgggc atgaattggg gacgaacctg
     1801 ctggtgctgt ggctggcacg cgagggaacg tacctgcgcg aggcgaagga tgccaggctt
     1861 gctacgcagc ggctggtgga tgcgctggat gccctgctgg actacgatcc acacgtgcgc
     1921 atcgccatcg agccgaaacc aaacgagccg atggacctgg cgtatgtccc gacgatggga
     1981 catggcatcg ccctgggtgc gcagacgaag gagccacgca gaatgggggt gctgatggaa
     2041 tccgcacacg caattctggc ggggcttgat ccctcggacg agattgcctt tgccctggct
     2101 ttcgataagc tgtggagtgt gcatctcaac gatcagaacg gcctgaaata cgatcaagac
     2161 cgctcgtttg gctcggttga cctgcgccgg gccttcaacc aggtgcgcgt actggacaac
     2221 aatcattact ggcagttcgg cattgtcggc ctggatgtga aggctatgcg cacacagccc
     2281 gccgagcttg ctaccaagca cctgcgtaac agcctgcacg tctttctgcg tctggtagag
     2341 atagtacgat ctatcgacca gcaccgcatc gatgctttga ttgccgcgag agattatgaa
     2401 gagctggatt gggtgatatt caattattta accggacgtt cataagagta caggagaacc
     2461 aagtatgccc gcttttgcac atcgcgtcga gccgttcaac tcgactattt tcactgagat
     2521 caacctgctg gcacagcaat acaacgcgct gaatctgggg cagggtaagc cggattggga
     2581 gacgccgccg gatatcgtcg agcagttggc gcgggcggtg caggcgcagc agtataacca
     2641 gtacgcgccg gggccaggca cactcacact gcgccgggcg gtggccgatc acgcggcgcg
     2701 cttttatgat atggatatcg acgcgggtcg cggggttgtc gtcacagcgg gggcgaccga
     2761 gggcatcttt tgtgccgtga tggggctggt cgatccagga gacgaggtca tcatgatcga
     2821 gccgttttac gattcgtacc cggcctcgat catgatggcg ggggcgacgc ccgtctgcgt
     2881 gccgctgcgt ccccccgatt ggaggctcga tcccgacgaa ctgcgggcgg cgtttacgaa
     2941 gaagacacgc gcgctgatcc tgaatacgcc gcataatccg acggggcgcg tcttctcgcg
     3001 gcaggaactc ggcatgatcg ccgacctgtg catcgagcat gatgtggtgg tcatttccga
     3061 cgaggtgtac gaacacttgc tgtatgacga tgccgaacat gtacccatcg ctacactgcc
     3121 gggcatgttc gagcgcacgg tcacggtaag cagtgccggg aagttgttca gcgccaccgg
     3181 ctggaaaatt ggctgggtct atggataccc tgaccttgta gacggcgtag ctagagcgca
     3241 ccaatttatc accttcgcag tg
//
LOCUS       DASZAK010000749         3143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_551104, whole genome shotgun sequence.
ACCESSION   DASZAK010000749 DASZAK010000000
VERSION     DASZAK010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3143
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551104"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1702)
                     /locus_tag="VGT44_13160"
     CDS             complement(<1..1702)
                     /locus_tag="VGT44_13160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2581796.1"
                     /translation="MVLALLLIAALSFGSFRSRSITIDEVVDIPSGLSYWQKQDTRLN
                     VEHPPLLKMIAAVPLLFAHVKVDYNDPSWCAGGSADCEWEFGKKLVEKWNANPQHLLI
                     LARIPMLGITLLLGWTIYAMARRLAGRWGGALSLVLFATSPFYLGLGPLVVTDIGLPL
                     FVLAAVWRFSSLWSHPNGRNTVWFALSLACALLSKFSALMLFPTFFLLWLYFRVSSWK
                     PGTRDQEPTSARRAHGFATLLKSFVAEWYAILGIALAGALVYLFYLLTCWHSDSIYIF
                     SARAHTFGSSKAGVITGRISLFLPKHPLLAKLLSPLWLYLSGILDVYFRIDRRTYVLG
                     KWHQHGVWYYFPVVSFFKLAPGMIFCIAFLVVLLGLYILRRCSARTPLVPQQYRRHLE
                     ALIVALIVFAASAISSTLNIGIRHFSVPITFLVLLCALVVPLMKAVLPPGVPRTLGAL
                     AASGMAVSSLATALLAYPHYIPYYNFFRLGTPKQEIANNSNLYWGQSLIDLERFRQEH
                     HISKIYVDSRTFRPDPAAYVSGASEWECGEPDPPAPEWAAVNATFLLRDAPTCAGLLR
                     Y"
     gene            2191..>3143
                     /locus_tag="VGT44_13165"
     CDS             2191..>3143
                     /locus_tag="VGT44_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEV2581797.1"
                     /translation="MAKHPPDRARGILTAYFSHLLVPLFIALDEVKFFLFEHVELHRS
                     VIDKIVLSCCVAWLAVSLGAFFLSKDRTLFSRRIEGPLISFYATLFVLVLFEGFVRIA
                     LRPAPPALWPPGTQWVFQPASWGVPVPGTTRFSVNELGLRGPALPRQGHPYKIVAVGG
                     STTACWFLDDSKEWPHLVMEGLNSRQKKLPVWVANAGVPGHNSVHHLAVLKTLPIFKQ
                     VDMVVILTGVNDLSASLAFEGAPSQTQLERSAELFREYMLRGAESERYLHPIYKRLRL
                     YQLAQQTKDSLLARFEHAGTGASETERELWQRRRAEGKIVSL"
BASE COUNT          760 a          849 c          890 g          644 t
ORIGIN      
        1 ggtagcgaag cagtccggcg caagtcggcg cgtcgcgcag caggaaagtg gcattaaccg
       61 ccgcccactc gggcgcgggc gggtccggct caccgcactc ccactccgag gccccggaga
      121 cgtacgctgc cggatctggt ctaaacgttc ggctgtccac gtagatcttc gagatatggt
      181 gctcctggcg gaacctctcc aggtcgatca aggattggcc ccaatacagg ttggaattgt
      241 tggcaatttc ctgcttcggt gttcccagcc ggaagaagtt gtaatacggg atgtaatgag
      301 ggtatgccag cagcgctgtg gccaggctgg aaaccgccat accgctggca gccagtgcgc
      361 ccagggtgcg aggaacgccg ggtggcagca ccgccttcat taggggaact acgagcgcgc
      421 acagcagaac cagaaaagta atcggaacgg aaaaatgccg aattccgata ttcaaagtcg
      481 atgatatcgc actcgcggca aaaacgatca gggctacgat tagagcctcc aggtggcgcc
      541 tatactgctg aggaacgagt ggggtccgcg cgctgcaccg gcgtaaaata tagagcccca
      601 gaagcacaac caggaaagcg atgcagaaga tcattcccgg agccaacttg aagaaggaga
      661 ccaccgggaa atagtaccag acgccatgct gatgccactt tcccagaacg taggtccttc
      721 ggtcaatcct gaagtaaaca tccagtatac cacttaagta cagccagaga ggactcagca
      781 gcttcgccag gagtgggtgt ttggggagaa ataaactgat acggcctgtg ataactccgg
      841 ccttcgacga cccgaaggtg tgcgctcgcg cagaaaaaat atagattgaa tcgctgtgcc
      901 agcacgtcag gagatagaat aagtaaacta aggccccagc tagagcgatc ccgagaatcg
      961 cgtaccattc ggcaacgaag ctcttcagca gcgtagcgaa gccatgggcg cgacgtgcgg
     1021 aggttggctc ctggtctcgt gtccctggct tccacgacga tacacggaaa tatagccaca
     1081 gtagaaaaaa tgttgggaag agcatcagag cagagaattt cgagagaagg gcgcacgcta
     1141 aagatagggc aaaccacacc gtattcctgc cattgggatg cgaccacagc gaagagaacc
     1201 gccacactgc agccagaaca aaaagcggta agccaatgtc ggtcaccacc aacggcccca
     1261 aacccaggta gaaaggcgag gtagcgaaaa gcactagact caatgcccca ccccaacggc
     1321 ctgccaggcg ccgcgccatc gcgtagatgg tccagcccaa gagcaatgtg atccccagca
     1381 tcgggattcg agccagaatc aacaagtgct ggggattcgc attccacttc tcaactagct
     1441 tcttgccgaa ctcccactcg cagtctgcgc tgccgcccgc acaccacgag ggatcgttgt
     1501 agtccacctt cacatgagcg aagagcaggg ggacggcggc tatcatcttg agcagcggcg
     1561 ggtgttccac gttcagacgg gtatcctgct tctgccaata gctcaaccct gaagggatat
     1621 ccacgacctc gtcaatcgtt atcgatcgtg agcgaaaact accgaaagag agcgcggcta
     1681 ttaacagaag agccagaacc acgagaatgc tgtctcccgt ggtacgagga taaggggaag
     1741 cgatctcgtc tctcgacttc tgtgcggtgg ctgacgaggt gggggtgaca agtacgcaag
     1801 ccataatgtc gcctcgacca gagtatccta cgcgctcgaa cggaaaactc aggtatcccc
     1861 tcgcgaccgc ttaccctgtc aagtctgcta atcaagcctc atataatata ccacgttgcg
     1921 gcatcgtcaa gtcatttgaa gtagtttctc ttcgcaatca gaggcaagac cgcgtgagct
     1981 gccgcgcctc gacgggcata atggtttgta ttggttcatt tccgaaaata ccttcttcaa
     2041 ctgcatattg atctaatcag tataggacat cacttggagg tattccttcg aattggaggc
     2101 cgtttcagcc gatcgaagtt tcctgcaaaa gaatttgaaa agatatatga ttgccgtacg
     2161 cttcgggatg aacttgcagg gattccggat atggcaaagc atccaccgga tcgggcgaga
     2221 gggattctaa cagcctattt ctcccatctt cttgtgcccc tctttatcgc cctggatgag
     2281 gtaaagttct ttctttttga acatgtggaa ttgcatcgct ccgtcatcga taagatcgtc
     2341 ctcagctgct gcgtggcgtg gctggcggtc agtcttgggg cctttttcct gagcaaggat
     2401 cggacgttgt tcagccgccg tatcgaggga cctctgatca gcttctatgc gacgctgttc
     2461 gtcttggtgc tctttgaagg atttgtccga atcgctttgc gacccgctcc tccggccctc
     2521 tggccacctg gcactcaatg ggtgttccag cccgcttcgt ggggggtgcc ggtgccaggg
     2581 accacgaggt tctccgtaaa cgaacttggc ctgcgtggac ccgccctgcc gcggcagggc
     2641 cacccctaca agatcgtagc cgttggagga agcacgaccg catgctggtt tcttgacgat
     2701 tcaaaggaat ggccgcacct agtaatggag ggtttgaata gccgccagaa gaagctgccc
     2761 gtttgggtag cgaatgcggg cgtgccaggc cataacagcg tgcatcatct cgcggtcttg
     2821 aagaccctgc caatcttcaa gcaagtggat atggtcgtga ttcttaccgg tgtgaatgat
     2881 ctaagtgcga gcttggcttt tgaaggagct ccctcgcaga ctcagttgga gcggagtgcg
     2941 gagctatttc gtgagtatat gctcagaggt gcggagagcg agaggtacct ccaccccatt
     3001 tacaagcgcc ttcgattgta tcaactggct cagcaaacca aggatagcct gctggccaga
     3061 ttcgagcatg cgggtacggg cgcatccgag accgaacgtg aactctggca gaggcgacgt
     3121 gcggagggta agattgtctc gct
//
LOCUS       DASZAK010000750         3815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_551110, whole genome shotgun sequence.
ACCESSION   DASZAK010000750 DASZAK010000000
VERSION     DASZAK010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3815
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551110"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VGT44_13170"
     CDS             <1..781
                     /locus_tag="VGT44_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581798.1"
                     /translation="PDWVQVVRVFLSEPSHSFASYRTRLAAPKEDQALPLRRLLLSDA
                     AVPQVCQAVFDAVMKNGASAHGVSSVIALAASDLLQGVGDDDAFVAASHALLFAAATR
                     IVYLQVQEVEALPLLLTAACAVNAMHKDLAQASQAAPSTASAPSRSSVLGGGLIAPAL
                     LESLGEQLDAQDLTGAFATARRYLQLGHDARALFAIIARSAAQADAAADGGHTLQIVQ
                     AAGETFLAWPTALRSTTIDGLLHTALRAAAFAKRNTLTAGL"
     gene            895..2610
                     /locus_tag="VGT44_13175"
     CDS             895..2610
                     /locus_tag="VGT44_13175"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2581799.1"
                     /translation="MRYNKTIFTIFTCSLLLLLLSSCDLFGGGNPTPTAQVKAAPDKQ
                     ILKIPMIGVSDITTFDPALAADQASISAVQMIFTGLVQLDDHQQVRPELAQRWSVSPD
                     GLSWTFRLKPGLKFSDGTPLTSMDVAYSIDRALQPTTKSAVAPIYLGLIRDADRLLLG
                     SITTLINDSLLTPDPQTIIIQTKKKAAYFLSMLAYPSSYVVEKRLVQTFGANFTDHLM
                     DGGGAGPFRVAQYTHGKAIDFVFNPNYVDARPQLREVIFTFYPQAAAAYNAYLSGDVD
                     MTGVPLATYASDRQRGDFHQVTQLWIDYYTMNYLVKPFDNIDVRQAFALAIDKTAITK
                     TVWNGAALPTNHIVPQGMSGYNPGLTGPDGTQSLQGNPQLAKSLLMRGLQAEGWASIA
                     QMPPIKLTYASDAGNFGAEVTAMIGMWQNALGVTVMPNPVPYNTLLAQVVAATGNQQG
                     LQMWGLTWAAEYPDPQDFLTRQFDAGVPNNNMNYGQNSSSDAAAQQRTQQQLETADAD
                     LQQQARLQTYQRAEQQLVKDVAWLPVDQLTRVYLLKPYVVGLPENAQGFVPPDDWANI
                     YIAQA"
     gene            3018..>3815
                     /locus_tag="VGT44_13180"
     CDS             3018..>3815
                     /locus_tag="VGT44_13180"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2581800.1"
                     /translation="MQSARKPRAWLISSLIFLVALLAACGGTTNPSTTPTTHTKASDA
                     QQVYTSGELYGLSDFGSLDPHLTSSLQDAFVADGIFVGMVQLNAQGALYCELCSSYSV
                     ASDGVTWTFNLRSGLKFSDGTPLTSADVVYSINRALDPATQSGTGPYYLRYIKDAIAF
                     AGGSKTIKTLIGDSLLAPDANTVKIIAASPVAFFLDALTYPTSYVVEQSVVKQWGKAW
                     TQHLSDNGGQGGAGPWKMLSYVPSKQAVLVPNPNYWGPKPQLKKIIFP"
BASE COUNT          778 a         1245 c         1078 g          714 t
ORIGIN      
        1 accagactgg gttcaggttg tgcgggtttt cctctcggaa ccatcgcaca gcttcgcgag
       61 ttaccgcacg cgcctggccg cgcccaaaga agaccaggca ttgccgctgc gccgcctgtt
      121 gttgagcgat gccgccgtgc cacaggtttg ccaggccgtc ttcgacgccg taatgaagaa
      181 cggcgcatcc gcccatggcg tcagttctgt catcgcgctt gccgcgtccg acctgttgca
      241 gggcgtcggt gacgacgacg cctttgttgc ggcctcgcac gcgctgctgt tcgcggcagc
      301 cacgcgcatc gtctacttgc aggtgcagga agttgaagcc ctgcccctgc ttctgacagc
      361 agcctgcgcc gtcaacgcca tgcacaaaga cctggcgcag gcgagccagg ctgcgccgtc
      421 aaccgcgtct gctccttcgc gctcgagtgt cctgggcggc ggcctgatcg ccccggcgct
      481 gctcgaatcg ctcggcgagc agctggacgc gcaggacctc accggcgcgt tcgcgactgc
      541 gcggcgttac ctccagcttg gccacgacgc gcgtgccctg ttcgccatca tcgcgcgctc
      601 tgccgctcaa gcggacgcag ccgccgatgg cggccatacg ctgcaaatcg tgcaggccgc
      661 cggagagaca ttcctggcat ggcccaccgc cctgcgttcc accaccatcg acggcctgct
      721 gcacaccgcc ctgcgcgccg ccgccttcgc gaaacgcaac accctgacag ccgggctatg
      781 atatgccaat cgaaatcaca atggatagaa gtgtaaagtc ccagggcggg gcggcgggga
      841 gtggggtgga gacccttgcg gtcgccctcg tcctctgtga tgaggagacc gataatgcgc
      901 tataataaaa cgatatttac tattttcact tgttctctcc ttctcctcct tctctcctcc
      961 tgcgacctgt ttgggggcgg gaatccgacg ccgacggcgc aggtgaaggc cgcgccggat
     1021 aagcagatat tgaagattcc aatgatcggt gtttctgata taacgacctt cgacccggcg
     1081 ttggcagccg accaggcttc gatcagcgcg gtgcagatga tcttcacagg tctggtgcag
     1141 ctcgacgatc accagcaggt gcggcctgaa ctggcccaga gatggagcgt ctctcccgac
     1201 gggttaagct ggacgtttcg cctgaagccg gggttgaagt tcagtgatgg cactccactg
     1261 acctcaatgg acgtggccta cagcatcgac agggcgctgc agcccaccac gaagtcagcg
     1321 gtcgcgccca tctatctggg gctgatccgc gacgcggaca ggctgctgct cggctcgatc
     1381 acaacgctga tcaacgatag cctgttgaca cctgacccac agacgatcat tatccagacg
     1441 aagaagaaag cggcctactt cctttcgatg ctggcctatc ccagttcgta tgttgtcgag
     1501 aaacgcctgg tccaaacgtt cggcgcaaac ttcaccgacc atttgatgga tggcggggga
     1561 gcaggaccct tccgcgtggc gcaatatacg cacggcaaag caatcgattt cgtcttcaac
     1621 cccaattacg tcgatgcccg accacagttg cgcgaggtga tcttcacctt ctacccccag
     1681 gcagccgctg cctacaacgc ctacctgagc ggcgatgtcg atatgaccgg cgtaccgctg
     1741 gccacctacg ccagcgatcg gcagcgcggc gattttcacc aggtaacgca gctttggatc
     1801 gactactata ctatgaacta cctggtcaag ccgttcgata atatcgacgt acgccaggcg
     1861 ttcgcgctgg ccatcgacaa aaccgccatt acgaaaaccg tctggaatgg cgccgccctg
     1921 cccaccaacc atatcgttcc gcagggcatg agcggctaca accccggact gaccggaccg
     1981 gacggcacgc agagcctgca agggaacccg caattggcga agagcctgct gatgaggggt
     2041 ctgcaagccg agggatgggc ttctatagcg cagatgccgc ccatcaaact gacctacgcc
     2101 tccgatgccg gcaatttcgg cgcggaggtc accgccatga tcggcatgtg gcagaacgcg
     2161 ctgggcgtca ccgtcatgcc caaccccgtt ccctacaaca ccctgctggc gcaggtcgtg
     2221 gcagcgacag gcaaccagca gggactgcaa atgtggggac tgacctgggc cgccgaatat
     2281 cccgaccccc aggatttcct gacgcgccag ttcgacgcgg gcgtccccaa caacaacatg
     2341 aactatgggc agaatagcag cagcgacgcc gccgctcagc agaggaccca gcagcagctc
     2401 gaaaccgccg atgccgactt acagcagcaa gcgcgcctgc aaacgtacca acgggcggaa
     2461 cagcaactgg tgaaggacgt ggcatggctg ccggtagatc aactcacgcg agtctacttg
     2521 ctcaagccct acgtggttgg cctgcccgaa aacgcgcagg gcttcgtccc tcccgacgat
     2581 tgggccaaca tctacatcgc acaagcgtag gaacgtgatg atgaacattc caaaccaaag
     2641 tcgggaatgt cacccttcgc tgcgctcagg gtctcggtcg gcggcacacg agatgcttcg
     2701 ctgcgctcag catgacagaa ctggtggtcg gtaacattca tcatcgcgtc cctaatttgc
     2761 ttgtgtgaga tttgccagat tccccgcgta atagtagtat gccaccgttt gcttccaacc
     2821 agacaggcaa gcggctcacc agcaccctac cgcgtttcac aggagtagtc gtgacgtata
     2881 actgcgcaga tgaatcgtga ctataccttc gagccgttga ctatattatg tagggagctg
     2941 aatctgccgg gcagcggcgc ggcaggtcga tatgttcgtt gtcgtctcca aacagttatt
     3001 taaggagaaa agattcaatg caatcagcca ggaaaccgag ggcatggctc atctcctccc
     3061 tcatcttcct cgtggccttg ctcgcggcct gtggcggaac gaccaatccg agtacgacgc
     3121 cgaccacaca caccaaagcg tcggacgccc agcaggtcta tactagcggc gagctatatg
     3181 gcctctccga cttcggttcg cttgaccccc acctcacctc ctccctccag gatgccttcg
     3241 tcgctgatgg catcttcgtg ggcatggtgc agttgaacgc acagggggcg ttgtactgcg
     3301 agttgtgtag ctcgtacagc gtggcctccg atggtgtaac ctggaccttc aatctgcgct
     3361 cggggctgaa gttcagcgac ggcacgccac tgacctccgc tgacgtggtc tacagcatca
     3421 accgcgcgct cgaccccgcc acgcagtcgg gcaccggccc ctactacctg cgctacatca
     3481 aggacgccat cgccttcgcg ggcggcagca agaccatcaa gaccctcatc ggcgacagcc
     3541 tgctcgcccc cgatgcgaac acggtcaaga tcatcgccgc ctcgccggta gccttcttcc
     3601 tggatgcgct gacctacccg acctcctacg tggtcgagca gagcgtcgtg aagcagtggg
     3661 gcaaagcctg gacccaacac ctctccgaca acggcggcca gggcggcgcc ggtccctgga
     3721 aaatgctgtc ctacgtgcct agcaagcagg cggtgcttgt ccccaacccc aactactggg
     3781 ggccaaagcc gcagctcaag aagatcatct tccca
//
LOCUS       DASZAK010000751         5091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_268659, whole genome shotgun sequence.
ACCESSION   DASZAK010000751 DASZAK010000000
VERSION     DASZAK010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268659"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VGT44_13185"
     CDS             <1..1187
                     /locus_tag="VGT44_13185"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581801.1"
                     /translation="VISFAPDVIVTQHGCDTHAWDPLTHLSLTMRGIQAQIKLAHELA
                     HNYCNGRWLALGGGGYDLYRVVPRAWSLLWAEMSQQSLPDALPETWIGRWRPAWKEIE
                     AQELAEQQIMGKAATSSLFPLNFLDRQEDFPAQPRRWSISSANRHTVGLLRHLLLPPS
                     IRQAFPSLQRQSPFAGLFDLLHLQGAATPSRSKTIETRAGSLLLRDFCPPSMVERLKP
                     DAGLHAFARLPDREHRLLLSIAKRPDCALTLAHTTEGMIVGEVTMAPGDEWWEGLENV
                     YEVAIQVSSNWRGHGVARELLGFALELDALEDMILFAIGLTWHWDTEGLGMNVYRYRQ
                     MLARLFGSQGFVEYPTTEPNVSMEPANVLLARIGKRVDQRTVSRFLNRLLASPNLTAF
                     NA"
     gene            complement(1325..1516)
                     /locus_tag="VGT44_13190"
     CDS             complement(1325..1516)
                     /locus_tag="VGT44_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581802.1"
                     /translation="MDRKEDREILRRGGFAESEMKRLSQLRKEINEQAKLSAIADRRR
                     MEFVRWLVRTGKLSEQIAS"
     gene            complement(1559..1939)
                     /locus_tag="VGT44_13195"
     CDS             complement(1559..1939)
                     /locus_tag="VGT44_13195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2581803.1"
                     /translation="MRKILLVDDDTSLARLEAGMLTARGYNVEIATSGELAISAIHQA
                     IPDLVLLDLDLAGPTSGWDVFKALRDLSRAPVLLTSAESAVRRHMRTQGESRSTLDHL
                     PKPYPMQALLKRIERMLAMEPQEP"
     gene            2560..3606
                     /gene="hemE"
                     /locus_tag="VGT44_13200"
     CDS             2560..3606
                     /gene="hemE"
                     /locus_tag="VGT44_13200"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEV2581804.1"
                     /translation="MEWSSGVGRMLAACRHEQPDTTPIWFMRQAGRCLAQYRELRTRY
                     DILTMAKTPDLCAQVTLMPVEQFGVDGAVLYADIMLPIEGMGISLEIEPDIGPIIHNP
                     VRTMRDVEALRILDAEEATPFVMDAIRLVRHELEGKQAVIGFSGAPFTLACYLIEGRP
                     SRDYGLAKSLMYGQPQVWHALMEKLTEVVIRYLLAQVRAGVDVVQLFDSWVGILSPAA
                     YARYAQPYSQRIFEAVRQSGVPSIHFGTGTASLLELMAEAGGDIVSVDWRVELDEAWE
                     RIGFERGIQGNLDPTMLLTSWECIERGMMDVLRRANQRPGHIFNLGHGVLAQTDPDML
                     RRLVDNVHRVTARK"
     gene            3617..4561
                     /gene="hemH"
                     /locus_tag="VGT44_13205"
     CDS             3617..4561
                     /gene="hemH"
                     /locus_tag="VGT44_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV2581805.1"
                     /translation="MTNRARVGVMLMTYGSPATLDDLPTYLSNVRGGRPADEALIAEF
                     RRRYDLIGGSPLLRITREQAAALQAELIRQHPDGPDFHVVAGMRFAPPYIADVAPEAA
                     AGASQLIGVIMSPQYSPIIMSGYVKALQAAVNTLDRPDLELRIAGDWHLQPLFLRALA
                     ERVRQALVQFPREERERVPVLLTAHSMPKRVVEKEEGYIQSLKDTAAAVARLVGLPAE
                     RWMFCYQSAGHTPEEWLKPDFADVMPELRAAGHTHVLIAPVQFLADHLEILYDIEIGA
                     REQAEAVGIHFARTASLNTSPLFIQALAEVVLHTREQG"
     gene            4563..>5091
                     /locus_tag="VGT44_13210"
     CDS             4563..>5091
                     /locus_tag="VGT44_13210"
                     /inference="COORDINATES: protein motif:HMM:NF017051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581806.1"
                     /translation="MWIAVVGIDHTTAPIDLREQLACSPARALEVMSAARPIGLECVL
                     LSTCNRIELYAAGSGRFEEEIETRLLQVLCVLGENRQVEIDEVRAHSYCFTGRTAVSH
                     LFEVACGLRSLILGEPQIQGQVAEALEQARLAGSSGPVVSALFRAAIVAGKRARSETG
                     ISRSAVSVSHVAVQLA"
BASE COUNT          996 a         1467 c         1572 g         1056 t
ORIGIN      
        1 tagtcatctc gttcgcgccg gatgtcattg tgacgcagca tgggtgcgat acacacgcct
       61 gggaccctct cacgcacctc agcctgacga tgcgcggcat ccaggcgcag atcaagctgg
      121 cgcacgaact cgcgcataac tattgcaacg ggcgctggct tgcgctgggc ggcggtggct
      181 atgacctgta ccgtgttgtg ccgcgtgcct ggagtttgct ctgggcggag atgtcccagc
      241 agtcgctgcc ggatgccctg ccggaaacct ggatcgggcg ctggcggcca gcctggaagg
      301 agatagaagc ccaggaactg gccgagcagc agatcatggg caaagcggcc acatcatctc
      361 tctttccact caattttctc gaccgccagg aggactttcc tgcgcaaccg aggcgctggt
      421 cgatcagcag cgccaaccgg cataccgtcg ggctgttgcg ccacctgctg ttgccgccgt
      481 cgattcgcca ggcgtttccg tcgcttcaac gccaatctcc atttgccggg ctatttgatc
      541 tcctgcacct gcaaggcgcg gccacgccat cgcgcagtaa gacgatagag acgcgcgccg
      601 gatcgctgct gctgcgcgat ttctgcccgc cttccatggt cgagcgcctg aagcccgatg
      661 ccggtctgca cgccttcgcg cgcttgccgg accgcgagca tcgcctgctg ttgagcatcg
      721 cgaagcggcc cgactgcgcg ctgacgctgg cgcacacgac cgaaggcatg atcgtcggcg
      781 aggtgacgat ggcgcctggc gatgaatggt gggaagggtt ggagaatgtc tatgaagtgg
      841 cgatccaggt cagctcgaac tggcgcggac acggcgtggc gcgcgagttg ctgggatttg
      901 cgctggagct tgacgcgctc gaagacatga tcctgttcgc tatcggcctg acctggcatt
      961 gggataccga gggattgggc atgaacgtct accgctatcg ccagatgcta gcccggctgt
     1021 tcggaagcca gggctttgtc gaatatccca ccaccgaacc gaatgtgagc atggaacccg
     1081 ccaatgtcct gctggcgcgt atcggcaaac gagttgacca gcgcaccgtc agccgcttct
     1141 tgaaccgcct gcttgcctca cctaacctga ccgcattcaa tgcgtaaggg gggagcgaaa
     1201 caaaagacgc acggcaggtg aagcagaacc gccgtgcgtc ttttgttttt ctcacaagga
     1261 ttccggttgg tacatttgac agcacccaga agtaaaaacg cgatatctat ctgcctgagt
     1321 attgttacga agcaatttgc tcagataact tgccggtacg taccagccag cgcacaaact
     1381 ccatgcgacg acgatcggcg atggcgctca gctttgcttg ctcgttaatc tctttgcgaa
     1441 gctggctcaa tcgcttcatc tcgctctcag caaatccccc cctgcgcaag atttcacggt
     1501 cttccttacg gtccatatga tcctccttac cgtcgctaac gtttcgatag agaatcgcct
     1561 acggttcctg tggctccatt gccaacatgc gctcaatgcg tttgagcagt gcctgcatcg
     1621 ggtatggctt cgggagatgg tccagggttg accgggactc gccctgtgtg cgcatatgcc
     1681 gacgcacggc tgattccgcg ctggtgagta gtactggcgc tctggacagg tcgcgcaggg
     1741 ccttgaacac gtcccaccca ctggtcgggc cggccaggtc aaggtcgagc aggacaaggt
     1801 ctggaatcgc ctggtgaatt gcgctgatgg ccagctctcc acttgtcgcg atctctacat
     1861 tgtagcctcg cgctgtcagc attccagctt ccagccgggc gagagaggta tcatcatcaa
     1921 ccaggagaat ctttctcaag attggtcgcc cctccgcacg cggatacaac gggttcggtc
     1981 gcgtcgcaat accatgcgta ccactcatgg tagcttcact caaagtacgt gtaccgctcc
     2041 ccggctgggt gaattgtgca gcgtccctat ttgtccctgc tgacatcggc tgacatcctg
     2101 cttccgcaaa attttcgttg tgttgcggta tcaccgccgg tgcggtgcga gagaatttca
     2161 ctctctcttg cagtaattgt acctgctcat cagtgagata tgtgtgaaga aatattcatc
     2221 aatgtgaaaa tgacgtgaag aatgaggaga agaagctgaa cagaaccctt gactattggc
     2281 gaaaaaacgc taaaatctct atagaattgt gtgtgtgtga agattttgtg agatatcggc
     2341 acacatccat attgaacaga gccagtaggg cgcgatgacg aagttttaca accaccatcg
     2401 ggagggcatt gtcatcctga gcgcagcgaa ggatctaatt cgccggggac taagatcctt
     2461 cgctgcgctc aggatgacaa tgcctggctt tagtcgttct aattcatcat tacgtcctgt
     2521 cagcgagagt taatagtgaa ttgattgaag gaacagtgta tggagtggtc ctctggagtt
     2581 gggcgtatgc ttgccgcttg caggcacgag cagcccgaca cgactccgat ctggtttatg
     2641 cgccaggccg gtcgctgcct ggcgcagtac cgcgaactgc gcacgcgcta tgatatactg
     2701 acgatggcga agacgcctga cctgtgcgcg caggtcacat tgatgccggt cgaacagttt
     2761 ggcgtcgatg gcgcggtact ctacgccgat atcatgctgc ccatcgaggg catgggtatt
     2821 tcactagaaa tcgagccgga tatcggcccg atcattcata atccggtgcg cacgatgcgc
     2881 gatgtcgaag ctctgcgtat cctcgacgcg gaggaagcaa cgccctttgt catggatgcc
     2941 atccggctgg tgcgccacga gttggagggc aagcaggcgg tgatcggttt ctctggcgcg
     3001 ccgttcacgc tggcatgtta cctgatcgag ggtcgcccat cgcgcgacta tggcctggca
     3061 aagtcgctga tgtatggcca gccgcaggtc tggcacgcgc tgatggagaa attgaccgag
     3121 gtggtgattc gctatctgct ggcgcaggtg cgcgcgggcg ttgatgtagt gcaactattc
     3181 gatagctggg tgggaatcct cagcccggca gcctacgcgc gctacgcgca gccgtattca
     3241 cagcgcattt tcgaggcggt caggcaaagc ggtgtgccat cgatccattt tggcacgggg
     3301 acggccagcc tgctcgaatt gatggccgag gcgggcggcg atattgtcag cgtggactgg
     3361 cgtgtcgagc tggacgaggc gtgggagcgc attggcttcg agcggggcat tcagggcaat
     3421 ctcgatccca cgatgctact gacctcgtgg gaatgcatcg agcgaggcat gatggatgtg
     3481 ctgcgcagag ccaatcagcg tccggggcat atctttaacc ttgggcatgg cgtgctggcg
     3541 cagaccgacc ccgacatgct gcggcggctg gttgacaacg tccaccgggt gacggcgaga
     3601 aagtagagca ataagcatga caaatcgagc gcgagtgggt gtgatgttga tgacctacgg
     3661 ctcaccggct acccttgatg atcttcccac gtacctcagc aacgtgcgcg gcggacgacc
     3721 cgcagatgag gccctgatcg cagaattcag gcgtcgctac gacctgatcg gcggctcgcc
     3781 gctgctgcgc atcacacgcg agcaggccgc cgcgcttcaa gcggaactga ttcggcagca
     3841 tcccgatggc cccgacttcc acgtcgtagc cggaatgcgt ttcgcgccgc cctacatcgc
     3901 ggatgtcgcg cccgaagccg ccgccggggc gagccagctc atcggcgtga ttatgtcgcc
     3961 gcagtactcg ccgatcatca tgagcggcta tgtcaaggcg ctgcaggccg ctgtcaatac
     4021 gctggaccgc cccgacctgg aactcaggat cgcgggcgac tggcatctcc aacccctgtt
     4081 tctacgtgcg ctggcggagc gggtgcggca ggcacttgtg caattcccgc gggaggaacg
     4141 cgagcgtgtg cctgtgctat tgaccgcgca tagtatgccc aaacgtgtcg ttgaaaagga
     4201 agaaggatat attcagagct tgaaggacac ggctgcggcg gtggcaagac tggtcgggct
     4261 gcctgcggag cgctggatgt tctgctacca gagcgccggt catacaccgg aggaatggct
     4321 caagccggac ttcgccgacg tgatgccgga actacgcgct gccgggcata cacatgtctt
     4381 gatcgccccg gtgcagttcc tggcggatca tctggaaatt ctctacgata tcgaaattgg
     4441 cgcacgcgaa caggccgagg cggtgggaat acacttcgcg cgcaccgcgt cactcaacac
     4501 ttcgccgctg ttcatccagg cgctggcgga ggtcgttcta cataccaggg agcaaggctg
     4561 acatgtggat tgccgttgtg ggcatcgacc atacgaccgc gcccatcgac ctgcgcgagc
     4621 agctggcctg ctcccccgcg cgggcgctgg aggtcatgag cgcggcgcgc ccgatcggcc
     4681 tggaatgtgt cctgctctcc acctgcaatc gcatcgagtt atatgccgcc ggttccggga
     4741 gattcgagga agagatcgaa acgcggctgc tgcaagtgct gtgcgtgctg ggagagaacc
     4801 ggcaagtcga gattgacgaa gtgcgggccc atagctattg cttcaccggc agaacggcgg
     4861 tgtcccatct cttcgaggtg gcctgtggat tgcggtcgct gatcctcggc gagccgcaga
     4921 ttcaaggaca ggtggccgag gcgctcgaac aggcgcgctt ggcaggctcc agcgggccgg
     4981 tcgtatctgc gctcttccgc gcggcaatcg tggcgggtaa gcgcgcgcgc agcgaaacag
     5041 gcatcagtcg cagcgccgtc tcggtcagcc atgtcgcggt gcagttggcg c
//
LOCUS       DASZAK010000752         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_624469, whole genome shotgun sequence.
ACCESSION   DASZAK010000752 DASZAK010000000
VERSION     DASZAK010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624469"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VGT44_13215"
     CDS             complement(<1..256)
                     /locus_tag="VGT44_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV2581807.1"
                     /translation="MSLTKRYDAGSAEAALHLIWQSSETYAFDRESQKPVYAIDTPPA
                     TVSGKLHLGHVYSYSQADFLARFWRMNGYNVFYPMGFDDNG"
     gene            complement(629..2113)
                     /locus_tag="VGT44_13220"
     CDS             complement(629..2113)
                     /locus_tag="VGT44_13220"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV2581808.1"
                     /translation="MVYPIHGMLHDEDDDQEVQGHLDSFDFEKQKYEMLIDRISGYSL
                     SRDYKTLVYRNGNRLRVVKAGDKPSKVEGDRAGRESGWLDLSRVKVSIQPAAEWKQMF
                     NEAWRLQREHFWTEDMSGIDWDAIYAQYAPLVERVSSRSELSDLFWEFQGELNTSHAY
                     EGGGDYRSRPYYRQGFLGVDWRYDGETQRYRIERIVIGDPADTKATSPLNSPGLNVVV
                     GDAVLAINGQRVGPGRSPRELLVNQAGNEVQLTIEDGKTRETRVITVKAISDEMPARY
                     REWVENNRRYVHEQSEGKVGYIHIPDMSANGFAEFHRTYLAEYDYPALLVDVRFNGGG
                     NVSGLLLEKLARRRVGYDFPRWGQPIPYPKESPRGPMVALTNEHAGSDGDIFSHSFKL
                     MGLGPLIGKRTWGGVIGISYRHALVDGTRTTQPEFAYWFKDVGWNVENYGTDPDIEVD
                     IAPQDYAASVDPQLDRAIAEALRMIEEKPALEPKPEERPRKVWR"
BASE COUNT          413 a          641 c          615 g          484 t
ORIGIN      
        1 ggccgttgtc gtcaaagccc atcgggtaga agacgttgta gccgttcatg cgccagaagc
       61 gcgccagaaa atctgcctgg ctatatgaat agacgtggcc gagatgcagc ttgccggaca
      121 cggtggccgg cggcgtatcg atggcataga ccggtttctg cgactcccga tcaaaggcat
      181 acgtctccga tgattgccag atcaggtgca gcgcagcttc ggcgctgccc gcgtcatacc
      241 gtttcgtcag actcatggtg gtctcctcca acaaaaaagc ccattcatcc catatgagga
      301 cgaaagggca taaatttcgc ggtaccacct caattcgcca ggctttgcgg cgctggcgca
      361 ctcgtcaccc tgacagttct ccaggcaagg agagttctat cggatctcgc tataacgggc
      421 ttacccgtgt cgatctactt tgcgccactt gtgagcgcat ttcttcagca gccacgccgg
      481 gcgacgttcg actgttgaga ctgcgggggc ttccagccgc tggccactcc ttctctctca
      541 gtgtatcgac agtttactct tcccggaggc gttgtatatt cgattgatgg ttttattgta
      601 catgggacac tgatgtgtgt caatggcctt atcgccagac tttcctcggt cgctcctccg
      661 gtttcggctc caacgccggt ttttcctcga tcatacgcag ggcttcggcg attgcgcggt
      721 cgagctgtgg atcaacgctg gcggcataat cctggggagc gatatcgacc tcgatatcgg
      781 gatcggtgcc gtagttttcg acgttccagc ccacatcctt gaaccagtag gcaaattcgg
      841 gctgcgtcgt gcgcgtgcca tcgacgagag cgtgacggta gctgatgccg atgacaccgc
      901 cccaggtgcg tttgccgatc aggggaccca ggcccatcag cttgaagctg tggctgaaga
      961 tatccccatc ggaacctgca tgttcattgg tgagagcgac catcgggccg cgcggcgatt
     1021 ctttgggata cggaataggc tggccccagc gcgggaagtc gtagccgaca cgccgccgtg
     1081 ccagcttctc cagcagcagg ccggagacgt tgccgcctcc attgaagcgc acatcgacga
     1141 gcaaagccgg gtaatcatat tcggccaggt aggtgcggtg aaattccgcg aagccgttag
     1201 cgctcatatc aggaatgtgg atgtatccga ccttgccttc cgattgttca tgcacatagc
     1261 ggcggttgtt ttcaacccat tcgcgatagc gcgcgggcat ttcgtcggag atggccttga
     1321 cggtaatcac gcgcgtctct cgcgtcttgc cgtcctcgat ggtcaactgc acctcgttcc
     1381 cggcctggtt caccagcagt tctcgcgggc tgcggcctgg cccgacgcgc tggccgttga
     1441 tggccagcac tgcgtcgccc acaaccacgt tgaggccggg actgttgagc ggcgacgtgg
     1501 ccttcgtatc ggcgggatcg ccaatcacga tgcgctcgat acggtagcgc tgcgtctcgc
     1561 catcgtagcg ccagtctacc cctagaaagc cctggcggta gtagggacgc gagcgataat
     1621 cgcctccccc ttcgtaagcg tgggaagtgt tcagttcgcc ctggaactcc cagaagagat
     1681 cggacaactc ggaacgtgac gagacgcgct caaccagtgg cgcgtattgc gcatagatgg
     1741 cgtcccaatc gatgccggac atgtcctccg tccagaaatg ctcgcgctgc aaacgccagg
     1801 cttcgttgaa catctgcttc cattccgcgg cgggctggat cgagaccttg acgcgcgaca
     1861 ggtcgagcca gccgctttct cgcccggcgc gatcgccttc aaccttcgac ggcttatcac
     1921 cggccttgac gacgcgcagg cgattaccgt tgcggtacac cagggttttg tagtcacgcg
     1981 acagagaata gccactaatg cggtcaatca gcatctcata cttttgtttc tcgaaatcga
     2041 agctatccag gtgtccctgt acctcttgat cgtcgtcctc atcgtgcagc atgccgtgaa
     2101 tgggataaac catgaagagc gcttttcctt taataccggc gatgccctag tat
//
LOCUS       DASZAK010000753         3811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_375449, whole genome shotgun sequence.
ACCESSION   DASZAK010000753 DASZAK010000000
VERSION     DASZAK010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375449"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="VGT44_13225"
     CDS             complement(<1..182)
                     /locus_tag="VGT44_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581809.1"
                     /translation="MSEQAVQSPLDITDGFIVTKAYRLFAEFCDACRKYQYIGICYGA
                     PGVGKTLSARHYARWD"
     gene            complement(175..1581)
                     /locus_tag="VGT44_13230"
     CDS             complement(175..1581)
                     /locus_tag="VGT44_13230"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2581810.1"
                     /translation="MERFYVIRPFLEDSVPLAQIARERAIPRRTLTRWVKQYRETGLG
                     GLIRQGRADKGERRNMPPDLKSLIEGLALSKPRRTRAAIYRQVMRVAKEQGWPMPSYS
                     QVSAIIDALDPGLVVLAHEGSKAYREQFDLLYRFEAEAPNHLWQADHTFLPVFLLNER
                     GKPDRPWLSAIMDDHSRAIVGYFLGFQAPTALQTALTLRQAIWRKDDPRWHVCGIPRV
                     FYTDHGSDFTSRHMEYVAADLKIELIFSEVGFPRGRGKIERFFQTMEQLLLPELPGYT
                     PEGQPPAPAQWSLPAFEEVFRTWLLDEYLKLPQKEIGAAPQARWEASGFLPQMPDSLE
                     QLDLLLLQVAKGRRVHQDGIYFEGKRYLDLTLAAYVGEDVTIRYDPRDMAEIRVFYHD
                     AFLCRAICAELAGQTISLKEIQAARTAQRKTLRETLTDRKLVVERYLAVHRPPETRSP
                     ESEPSTPPQPRLKRYLNE"
     gene            1967..2290
                     /locus_tag="VGT44_13235"
     CDS             1967..2290
                     /locus_tag="VGT44_13235"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEV2581811.1"
                     /translation="METSDLLESVPRALVHLAYALRGLANNQASVLAAGRTVRDVIDS
                     LERDFPGMRFHLCYETGELRPYVNIFLNRENIRYHQGLDTPLPYGAILHILPSVAGGT
                     LSSVR"
     gene            2327..3433
                     /locus_tag="VGT44_13240"
     CDS             2327..3433
                     /locus_tag="VGT44_13240"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV2581812.1"
                     /translation="MNARTTFPTGTTVLLVGTKRGLFLLSSRDRKAWDVSTTSLNGNR
                     VYFATLDQRQKQRLFATDNGDFFGTYLRYSDDFGQTWQEPEQGIQFSPESDRKLINIW
                     TIEPGRPDEPDTVYAGVDPASLWVSSDGGRHWSLNEGLESHPTRSEWNPGAGGLCLHT
                     IVADPSNANRMWIGISAVGCMRTDDGGKSWVFANKNTRADFQPEKYPEFGQCIHRFVQ
                     HPQQPDILYQQNHCGIYKTLNAGEDWIDIQHELPSDFGFPIALDAHNPDTVFVIVESQ
                     MGRNNVSDHFTVYRTVNGGDSWQPMTEGLPGGPGVRLGVLRHGMCTDTSDPCGVYVGT
                     NTGQIFASRDRGESWNLIADFLPSVYSVQAFVIE"
     gene            3551..>3811
                     /locus_tag="VGT44_13245"
     CDS             3551..>3811
                     /locus_tag="VGT44_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581813.1"
                     /translation="MIKITLSYRTAVLCVALVALAIAGTVFAQTSGASSGSDAGRMVR
                     SQSFQPLTLSQMGQAHQLASSVQVLSSQAPSVIIQFPLFPSGV"
BASE COUNT          855 a         1133 c         1003 g          820 t
ORIGIN      
        1 aaatcccacc tggcgtaatg tctcgccgaa agcgtcttcc caacgcctgg cgccccatag
       61 caaatcccaa tatattgata tttgcggcag gcgtcgcaaa attcggcaaa gagccggtac
      121 gccttcgtga cgataaaccc atcggtgata tccaaagggg actggacagc ctgttcactc
      181 attgagatac ctcttcaaac gtggttgtgg gggagtcgaa ggctccgact ccggtgagcg
      241 cgtttcgggt ggccgatgaa cggcgagata ccgttccacc acaagcttcc gatcggtcag
      301 ggtttcgcgc agggtttttc gttgcgcggt gcgtgcggcc tggatctctt tgaggctgat
      361 cgtttgccca gccagttccg cacaaatggc tcgacacaaa aaggcatcat gataaaagac
      421 ccggatttcg gccatatctc tgggatcgta ccggatggtg acatcctctc ccacatacgc
      481 ggcgagcgtc agatcgaggt agcgcttccc ttcaaaatag atgccgtcct ggtggacccg
      541 ccgtcctttt gccacttgca gcagtaacaa atcgagttgt tccagagaat ctggcatctg
      601 tggcaagaaa ccactggcct cccagcgtgc ctggggcgct gctccgattt ccttttgcgg
      661 cagcttcaga tactcatcca acaaccaggt gcggaacacc tcctcaaaag caggcaagct
      721 ccactgagcg ggagcaggag gctgcccttc tggggtatag cctggcagct ccggcaggag
      781 cagttgctcc atcgtctgaa aaaagcgctc gatcttccct ctcccacgcg gaaagcccac
      841 ttccgaaaag atcaattcga tcttcagatc agcggccacg tactccatat gtcgagaagt
      901 aaaatcgctg ccatgatcgg tataaaaaac acgagggatc ccacacacat gccagcgtgg
      961 atcatctttg cgccagatcg cctggcggag cgtcaacgcc gtttggagtg cagtcggtgc
     1021 ctgaaaaccc aagaaatagc caacaatggc acggctatgg tcgtccatga tagcacttaa
     1081 ccagggacga tctggttttc cccgttcatt cagcagaaag actggaagaa aggtatggtc
     1141 cgcttgccag aggtgattag gtgcctcagc ttcaaagcga tagagcaggt caaattgctc
     1201 tcgataggct ttcgaccctt catgtgctaa aacgaccaaa ccgggatcaa gcgcatcaat
     1261 gatcgcagac acctggctgt aactgggcat gggccagcct tgctctttcg ccactcgcat
     1321 cacttgccga tagatcgcgg cgcgagtgcg ccgaggcttc gaaagcgcca gtccttcgat
     1381 gagtgacttc agatcgggag gcatgtttcg ccgttctcct ttatcagcgc gcccctgacg
     1441 gattaacccg cctaagcccg tttcccgata ctgcttcacc catcgcgtca gggtccgtct
     1501 gggaatggct cgttctctgg caatctgagc caggggaaca ctatcctcta aaaacggacg
     1561 aatcacataa aagcgctcca acgcttgcgt tcgttcgctt tcagatgctc cgctatcctg
     1621 tggtaactca ctatcagcca tagccctatt ctaccagtcc acacgaccaa ccttcttcat
     1681 tcaaagagag tctaggagaa atgggccaaa tacttcctca aattcggagc catttaattt
     1741 ttgaaatgac atcactgagc tatttttcac gagcaagtag tttcgatctt cccccaatcg
     1801 gaatgtcctg agtagtttat tttattgcag atcacgtcta taagaaagac gtactgccct
     1861 ttccgcccga atagttctca tctcaaaggc ttgccatgat ttgattcgtc tgctatactg
     1921 aattgtagaa caaacgtcct gtataacccc gtcggaggtt cgcttcatgg aaacgtccga
     1981 tctgctcgaa agtgtgccgc gcgcactggt gcatcttgcc tatgcgttgc gcggtctcgc
     2041 caataaccag gcgtccgtgc tggccgccgg tcgcacggtg cgcgacgtaa tcgactcgct
     2101 cgaacgagac ttccctggta tgcgcttcca cctctgctac gagaccggcg agctgcgccc
     2161 ctatgtcaac atcttcctga atcgcgaaaa cattcgttat caccaggggc tggacacacc
     2221 cctaccctat ggcgcgatcc tccacattct gccatcggtg gccggtggca cactctcttc
     2281 cgtccgatga caacttttgg aacatcaatt ctaaggaggt ttactcatga acgctcgcac
     2341 aaccttcccc acaggcacga cggtgctgct cgtcggcacg aaacgcggac tcttcctgct
     2401 ctcctcgcgg gatcgtaaag cgtgggatgt ctccactacc agcctgaacg gaaaccgcgt
     2461 ctacttcgcc acactcgacc agcgtcaaaa acagcgcctc ttcgccaccg acaacggcga
     2521 tttcttcggc acctacctgc gctatagcga tgactttggg cagacctggc aggaacctga
     2581 gcagggtatc cagttctcac cggaaagcga ccgcaagctc atcaacatct ggacaatcga
     2641 gcctgggcgc cccgacgagc cggacacggt ctatgcagga gtcgatccag ccagcctgtg
     2701 ggtcagcagc gacggcggac gtcactggag cctcaacgag ggcctggaaa gccatccgac
     2761 acgctcggag tggaatccgg gcgcgggagg gttatgcctg catacgattg tggccgaccc
     2821 cagcaacgca aaccgcatgt ggatcggtat ttcggcggtg ggctgtatgc gcaccgatga
     2881 cggcggcaag agctgggtct tcgccaataa gaacacgcgc gccgatttcc agccggagaa
     2941 atacccggaa ttcgggcagt gcatccatcg ttttgtccag catccccagc agcccgacat
     3001 cctctaccag caaaaccact gcggcatcta caaaacgttg aacgcgggcg aggactggat
     3061 cgacatacag cacgaactgc cctccgactt cggcttcccc atcgccctgg atgcacacaa
     3121 ccccgacacc gtctttgtga tcgttgaatc gcagatggga cgcaacaacg tcagcgatca
     3181 cttcaccgtc taccgcaccg tcaatggcgg cgacagctgg cagccaatga ccgagggact
     3241 gccgggaggg ccaggagtgc gcctgggagt gctgcgtcac ggcatgtgta ccgatacgag
     3301 cgacccttgc ggtgtctatg tcggcaccaa taccggccag atcttcgcca gccgagaccg
     3361 gggcgaaagc tggaacctga tcgccgactt cctgccgtcg gtctactcgg ttcaggcgtt
     3421 tgtgatcgaa taaaacctct catgaaaatg ggtaatcttg cgcatgtttc tcttggagaa
     3481 accctgtacg cttacagaag aggaatagct ctggttggag tatcagggct gccaatcaga
     3541 aggagcgatt atgatcaaga ttaccctttc ctatcgtacc gccgtgctat gtgtggcgct
     3601 ggtggcgctg gccatcgccg ggacggtctt cgcgcagacg agtggcgcaa gttcgggcag
     3661 tgatgccggt cgaatggtgc gcagtcagag cttccagccg ctgacgctga gccagatggg
     3721 acaggcgcat caacttgcct cgagcgtgca ggtgctgtcc tcgcaagcgc ccagcgtcat
     3781 catccagttc ccgcttttcc ccagcggggt c
//
LOCUS       DASZAK010000754         2026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_588122, whole genome shotgun sequence.
ACCESSION   DASZAK010000754 DASZAK010000000
VERSION     DASZAK010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588122"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1704)
                     /locus_tag="VGT44_13250"
     CDS             complement(<1..1704)
                     /locus_tag="VGT44_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV2581814.1"
                     /translation="MQMQPIKKQAVNAGGNGGSGGIASIGAVGVQHRPDLVPLTEERV
                     RFEIDMCIGCDRCMRACPVPMSSMVNIADLNRATVSDDIAPHVARFTEECVMCGSCVP
                     VCPVDNHRDLLMLSLKRRLGIQWDGQVDMSRILQYLPPGWDVRLLLARLREMPMFSDP
                     QLVPENYLLHMVATSKLLSLLPGAVIIREGEYSRALYLILEGRFELSSAGADGGDLPI
                     AVLRRGEYMGEQSMLTGQTRNATARAQTAGVAIEVPEQVMQRMMELVPAVRRFFEQLN
                     DARSIESILKRMALFQGVPAADIRLVAEQTSVKRYDREERLFAEDGTGGPARDSMHII
                     LEGFVKVARRASNGTMHNLTGERILAYRQGGDYFAGGLDLLGDGRAVSVTAITRTRVA
                     EIPPAALANLFTRYGEVRQRFSMRLQQYRDAAAAAQTGFFDPLAHTSTAAVAAGSDAD
                     ARAGLRSLVGGGVVEGTEVLVIDLDKCVHCNECEEACARRHGHSRMNRKGMVIGNISI
                     ATACRQCQDPVCMLCSRAGIARLPSGEVYITESCIGCGICAERCPYDNISIMTLDDER
                     HG"
     gene            complement(1709..>2026)
                     /locus_tag="VGT44_13255"
     CDS             complement(1709..>2026)
                     /locus_tag="VGT44_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEV2581815.1"
                     /translation="DLGSSNGVMVNAMKIDGPYHLLHCDRILIGSVAIYFMHTALEES
                     FETVRASGAGGAVAKQVAAAMDAAWQCQSCGSSNLPVARFCANCGSPRAQQALAMSQG
                     KGR"
BASE COUNT          401 a          687 c          577 g          361 t
ORIGIN      
        1 cccgtgacgc tcatcgtcga gcgtcataat cgagatgttg tcatacgggc agcgttcggc
       61 gcagatgccg cagccgatac agctttcagt gatatagacc tcaccgctcg gcaggcgcgc
      121 aatacccgcg cggctgcaca gcatacacac ggggtcctgg cactggcgac aggcggtggc
      181 gatgctgata ttgccgatca ccatgccctt gcggttcatg cgcgaatgcc catgccggcg
      241 cgcgcaggct tcctcgcact cgttgcaatg cacgcattta tccaggtcga tcaccagcac
      301 ctcggtgcct tcgacgacgc ctccgccaac cagcgaacgc aatccagcgc gcgcatccgc
      361 gtccgaaccg gcagccaccg ccgccgtgct ggtatgcgcc agaggatcga aaaagccggt
      421 ctgcgcggcg gctgccgcgt cgcgatattg ttgcaagcgc atactaaagc gctgtcgcac
      481 ctcgccatag cgtgtgaaga gattcgccag ggccgccggt ggaatctcgg caacgcgcgt
      541 gcgcgtgatg gctgtcacgc tcactgcgcg tccatcgccc agcaggtcca gcccgcccgc
      601 gaagtagtca ccgccctggc ggtaggccag aatgcgctcg ccggtcaggt tatgcatcgt
      661 cccattcgaa gcgcgccgcg ccaccttcac aaaaccttcc aggatgatgt gcatcgaatc
      721 acgcgccgga ccacctgttc catcctccgc aaaaagccgc tcttcgcggt catagcgttt
      781 gaccgatgtc tgttcggcca ccaaccggat gtcggcggcg gggacgccct ggaagagcgc
      841 catacgcttc aggatcgact cgatagaacg cgcatcgtta agctgctcaa agaaacgccg
      901 cacggctggc accagctcca tcatgcgctg catcacctgc tccggcacct cgatggcgac
      961 gccagccgtc tgcgcgcgcg cagtcgcgtt gcgcgtctgc cctgtcagca tactttgctc
     1021 gcccatatac tcgccgcgcc gcagcaccgc gatgggcaga tcgccgccgt ccgcgcccgc
     1081 tgatgacagc tcgaaacgtc cctccaggat caggtagagc gcccggctat actccccctc
     1141 gcgaatgatc accgcgccgg gcagcagcga gagcaatttc gaagtcgcaa ccatgtgcag
     1201 caggtaattc tcaggcacaa gctgcgggtc gctgaacatc ggcatttcgc gcaggcgtgc
     1261 cagcagcagc ctgacgtccc aaccgggtgg gagatattgc aagatgcggc tcatatccac
     1321 ctggccatcc cattgaatgc ccaggcggcg tttgagcgag agcatcaaca ggtcgcgatg
     1381 gttatcgacc gggcagacag ggacgcacga gccgcacatc acgcactcct cggtgaaccg
     1441 tgccacgtgc ggggcgatat catctgagac agtggcgcgg ttgagatccg cgatattgac
     1501 catcgaggac atcggcactg ggcaggcgcg catacagcga tcgcagccga tgcacatatc
     1561 gatctcaaag cgcacccgtt cttcagtcaa aggaaccagg tccgggcgat gctgcacgcc
     1621 gaccgcgcca atactcgcaa tgcctccact gcccccgttg ccgccagcgt tcacagcctg
     1681 ttttttgata ggttgcatct gcatggaact acctgccttt cccttgcgac atcgcgagag
     1741 cctgttgcgc cctgggtgat ccgcagtttg cgcagaaacg cgccaccggg agattcgacg
     1801 acccgcagct ctggcactgc cacgctgcat ccatagctgc ggcaacctgc ttagccacag
     1861 caccaccagc accgctggct ctgaccgtct cgaacgactc ctcaagcgcg gtatgcatga
     1921 agtagatggc aacgctgccg atcaagatac ggtcgcagtg caacaggtgg tacgggccat
     1981 cgatcttcat cgcgttcacc atcacaccgt tgctgctgcc caggtc
//
LOCUS       DASZAK010000755         3188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_125063, whole genome shotgun sequence.
ACCESSION   DASZAK010000755 DASZAK010000000
VERSION     DASZAK010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3188
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125063"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VGT44_13260"
     CDS             complement(<1..596)
                     /locus_tag="VGT44_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581816.1"
                     /translation="MTNKHPDQPTASSGTLPKGVHLVGSIPLSSAEDVFRTTSSMLGG
                     RLRRVPDGETGIRSGWIGWQYPLFASNPSFETVAPPPNAYVPRSRFKLRSPRPSGAIT
                     FEQLGYADAALDSYRLFSQLKNDGIIPAQYRFQVCLPTPLAPISSFIAVEDQAEVEPA
                     YEAAMLRDLERILDAIPQDDLAIQWDTAAEFIILESVAP"
     gene            801..1760
                     /locus_tag="VGT44_13265"
     CDS             801..1760
                     /locus_tag="VGT44_13265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2581817.1"
                     /translation="MASWQYTRGLHDLGNAVYAYLQPDGTWGWSNAGLITDGETSLLV
                     DTLFDLKLTQEMLDTMRRAVPAASRIDMVVNTHANGDHCYGNQLVSDARRMIASRRTV
                     EEMKTAITPEQFAQLIKQAPLMGQVGQFVLHAFGKFDFENNTLTLPGETFDGEMTLQV
                     GSKEVRLIEVGPAHTMGDTMVYVPGDRVVFTADILFIGGHPIVWVGPIGNWLRACDRI
                     LAMDVDVIVPGHGPITDKQGVIGLRDYLQYVYDEARKRYEAGMSALEAARDIPMERYA
                     TWTDGERIVVNVAAVYRELSGDTSHPNMQGLIAQMAELYATMK"
     gene            complement(1785..2729)
                     /locus_tag="VGT44_13270"
     CDS             complement(1785..2729)
                     /locus_tag="VGT44_13270"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV2581818.1"
                     /translation="MSTNAPVTRQKSWAETPPQNPLLRHLISEVVENQTWLERLATPV
                     QNWLLRFFGQPGQPARKLKDMLNGTWFGHPLHPALTDVPLGAWSGTTLLDLAWLASED
                     DGIARSADLTLVLGLAGATGAVVTGVADWSDLDGTDRRVGLMHGLLNAGITTLYLASL
                     LMRLMGKRRSAITLSTTGYLASFLSGYLGGELSLAKGIGVNHTAWEGGSDDFVAVIDE
                     QELQAGKLTRVDVSGIPAVLLKEGKSIYAIGATCTHLGGPLDEGTYKDGVVTCPWHGS
                     GFCITDGSVANGPAVYAEPTFAVRVRAGKVELRRLDHA"
     gene            complement(2760..>3188)
                     /locus_tag="VGT44_13275"
     CDS             complement(2760..>3188)
                     /locus_tag="VGT44_13275"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2581819.1"
                     /translation="STATGRIITCRGRFDALPLQDESVDLALSCSAFTANPEQGGEPG
                     LAELQRVTRPGGKIVLIWPRIEDHNWLTSHGFTYVALPMPQVMTVRFRSQQSALACVR
                     RFYARNQALAPYILNRGAPEIPFSVLGINPPHDYCWRTVN"
BASE COUNT          690 a          915 c          970 g          613 t
ORIGIN      
        1 ggcgccacgc tttccaggat gataaactcc gcggcagtat cccattgaat ggccagatcg
       61 tcttgcggaa tggcatcgag aatgcgctca agatcgcgaa gcatggccgc ctcatatgct
      121 ggttctactt cggcctggtc ctctactgcg ataaacgacg agataggcgc gagaggcgtt
      181 ggcagacaca cctgaaagcg gtactgcgcc ggaatgattc catcgttttt gagttgggag
      241 aacaggcgat aggagtccag ggcagcatcg gcatacccca gttgctcaaa agtgatcgcc
      301 ccggacgggc gtggggaacg tagcttgaag cgcgaacgcg ggacataggc attgggtggg
      361 ggcgcaaccg tctcaaaact ggggttgctg gcgaacagcg ggtattgcca gccgatccag
      421 ccgctgcgta taccggtttc gccatccggg acgcggcgca gtcgcccgcc aagcatcgaa
      481 ctcgttgtcc gaaaaacgtc ctccgcgctg ctcaggggaa tgctgccgac aagatgcaca
      541 cctttcggca gcgtgccgga ggaggcggtt ggctgatcgg gatgtttgtt ggtcatgttg
      601 ccctccactc gttctaatct ggcaagagaa gcaaaaatag taggatagcc agatatattt
      661 tatcacgtgg taatgccact tcctcccacc cgggattgcg agtaattcct acaccgtccc
      721 acccacacca tttgacagcc ctgctagctc aacgtagaat gaggtacgac acgcaacagc
      781 ggaccgaaag gagatgcgct atggcttcct ggcaatacac gcgcggcttg cacgatctcg
      841 gcaacgcggt ttacgcctat ttgcagccgg acggcacgtg gggatggagc aatgcaggac
      901 tgattaccga tggcgagaca tcgctgctgg tcgatacgct tttcgacctg aaactgacac
      961 aggagatgct cgatacgatg cgccgggcgg tgcctgccgc gtcgcgcatc gatatggtgg
     1021 tcaacaccca tgccaacggc gaccattgct acggcaacca gcttgtgtca gatgcgcgac
     1081 gcatgatcgc ttcgcgcaga acggtggagg agatgaagac ggctatcacg ccggagcaat
     1141 tcgcgcagtt gataaagcag gcccccttga tggggcaggt tgggcagttc gttctgcacg
     1201 ccttcggcaa attcgacttc gagaataaca cgctgacgct gccgggggag acctttgatg
     1261 gcgagatgac gctgcaggtg ggcagcaaag aagtgcggct gattgaggtc ggcccggcgc
     1321 atacgatggg cgatacgatg gtttatgtgc ctggcgaccg cgtggtcttc accgccgata
     1381 ttctcttcat tggcggccat cccatcgtgt gggtcgggcc gatcggcaac tggctgcgcg
     1441 cctgcgaccg catcctggcg atggacgtgg atgtgattgt ccccggtcac ggtcccatca
     1501 ccgacaagca aggcgtgatc ggcctcagag actatctgca atatgtctac gacgaggcgc
     1561 gcaaacgcta cgaagccggg atgtccgcgc ttgaggcagc cagggacatc ccgatggagc
     1621 gttacgcgac gtggacggac ggcgagcgca tcgttgtcaa cgtggccgcc gtgtaccgcg
     1681 aactgagcgg cgatacctcg caccccaaca tgcaggggtt gatcgcgcag atggcggaac
     1741 tgtatgcgac gatgaagtaa acgctaagtc gcacagcgcc aggtctaggc atgatccagg
     1801 cggcgaagtt cgactttgcc agcgcgcacg cgcacggcga atgtcggctc ggcatagacc
     1861 gccgggccat tggccacgct gccatcggtt atgcagaagc cggagccatg ccagggacag
     1921 gtaacaacac catccttgta ggtgccttca tccagagggc cgcccaggtg cgtacaggtt
     1981 gcgccaatgg cgtagattga cttgccctct ttcagcaaga ctgccgggat accggataca
     2041 tcgacccgtg tcaattttcc cgcctgtagc tcttgctcat caatgacggc tacgaagtca
     2101 tctgagccgc cttcccaggc agtatggttc acgccaatgc ctttggctaa ggataactcg
     2161 ccgcccaggt aaccagagag gaacgaggcc agatagcctg ttgtggagag tgtaatggca
     2221 ctgcggcgtt tgcccatgag ccgcatcagc aatgaggcga ggtacagggt cgtgataccg
     2281 gcattgagca ggccatgcat gaggcccacg cggcgatcgg tgccatccag atcgctccaa
     2341 tcagccacgc cggtgaccac tgcccccgtt gcgcccgcca gtcccaacac gagcgtcagg
     2401 tcagcgctgc gagcaatacc atcatcttcg ctcgcaagcc aggcgaggtc cagtaatgtt
     2461 gtgccactcc atgcgcccag cggcacatcg gtcaaggcgg gatggagcgg atgtccaaac
     2521 catgtcccgt tgagcatatc cttgagcttg cgtgccggct gccccggttg cccaaagaag
     2581 cgcaaaagcc agttctggac gggtgtggcc agcctttcaa gccacgtttg attctcaacc
     2641 acctcgctga taagatgccg taagagcgga ttctgcggag gcgtctctgc ccaactcttc
     2701 tggcgcgtca ctggcgcatt tgtagacatt ggttccattc ctttcaactc aagagacctt
     2761 caattgacgg tacgccaaca ataatcatgc ggtgggttga tgccgaggac ggaaaatgga
     2821 atctccggcg ctccccggtt gaggatgtag ggagcaagcg cctgattgcg agcatagaaa
     2881 cgccggacac aggcgagcgc actttgctgc gagcgaaaac ggaccgtcat cacctggggc
     2941 atcggcaatg cgacataggt gaacccatgc gacgttagcc agttatgatc ctcgatacgc
     3001 ggccagatca agacaatctt gccgcccggc ctggtgacgc gctgcagctc ggccagacct
     3061 ggctcaccac cctgctccgg gttggcggtg aaggccgagc aggaaagggc gaggtccacg
     3121 ctctcgtctt gcagcggcaa tgcatcaaag cggcctcgac aggtgataat acgtcccgta
     3181 gcggttga
//
LOCUS       DASZAK010000756         4404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_125074, whole genome shotgun sequence.
ACCESSION   DASZAK010000756 DASZAK010000000
VERSION     DASZAK010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125074"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..773
                     /gene="rlmB"
                     /locus_tag="VGT44_13280"
     CDS             24..773
                     /gene="rlmB"
                     /locus_tag="VGT44_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV2581820.1"
                     /translation="MPEYVWGRNPVLETLRSPRRVKRILLADGQRESPVMVAIAQEAE
                     RRHIPIETTPRQRLDHISQGAVHQGCIAVVEARAYADLDDILAFAQQKQEDPFLLILD
                     AIQDVNNLGSLLRSAEAAGAHGIVIPLHEAADVNATVVKTSAGASEHLLIAQETNLTR
                     VIEYLKKQNVWVIGLAGEASTFYDQADLTGPLALVVGNEGKGISRLVREHCDLLIKLP
                     MHGVINSLNAAVAGSIALYEALRQREKKASK"
     gene            complement(779..1870)
                     /locus_tag="VGT44_13285"
     CDS             complement(779..1870)
                     /locus_tag="VGT44_13285"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HEV2581821.1"
                     /translation="MRSYRLAALPGDGIGPEVISESTQTLMNLADLHGGFRFDIQEFD
                     WGTERYLREGSLMPTDGLRVLERGNFDGILMGPIGDPRVPDHITLWGLLLPIRQGFDQ
                     YVNLRPMRLLPGVRSPLAGKRAGEIDIVCVRENTEGEYAGVGGRVHQGAEEEVAIQST
                     VFTRKGTERIIRYAFEYARQHKRKKVTSATKSNSMQYNMVFWDDVYRQVAPDYPEIMY
                     EQQLVDSLAARFVSRPESLDIVVASNLFGDILTDLGAAVTGSLGLAPSANLNPERSYP
                     SLFQAIHGSAPDITGKGIANPVASIWSGQLLLDFLGEREAAATLMRAIEETLAGGQVL
                     TPDLGGTSTTRQAGQAIRAKMREIVDSGG"
     gene            complement(1918..3165)
                     /locus_tag="VGT44_13290"
     CDS             complement(1918..3165)
                     /locus_tag="VGT44_13290"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581822.1"
                     /translation="MSRNERDTRSEPGEGQEPADEIAAGYLGVSPPVERLPEFGGQGG
                     HGTRAWVDRPEQLAMAADALRQAQVIAIDAEFAQIRSHAQGTAASSGPRLALLQLAIE
                     GQCFVVDALRVHDLSLLASVVGNPAISILLHGAGADMRVMSERGLVVAHYYDLEAASR
                     SIFGQRESSLAAMLLRAFNVRLDKSLQRTDWTRRPLPSAMIAYAARDAEMTLALYNWL
                     QRHYATFLRLHDSVNLEAPVAAWIEPHLRGHSPMSPDVALAEAILAGTVASQEQALRD
                     CEAALAGLVHPMLRSRLLRLITDLNFTAMAPAIEPILQASTFDERAAAVRALSRLDGE
                     RYKDAILPLLQDPVFDVRKAAHIALRATEPKEPKIQRAAPVRRADGSRSWTVGGNAET
                     QDGDEGDGGADDWKARLRSIMGE"
     gene            3357..>4404
                     /locus_tag="VGT44_13295"
     CDS             3357..>4404
                     /locus_tag="VGT44_13295"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEV2581823.1"
                     /translation="MANNVPITVAYGDGIGPEIMDATLRIITEAGARIETEVIEVGEK
                     VYLKGISAGIEPSAWESLRRTKVFLKAPITTPQGGGYKSLNVTTRTTFGLYANVRPCV
                     SYYPFVATKHPTMDVVIMRENEEDLYTGIEYRQTHDMVESLKLISRPGSEKIVRYAFE
                     YARSHNRKKVTCFMKDNIMKLTDGLFHKVFNEIANDYPDIENEAWIVDIGSAKLADTP
                     EAFDVIVLPNLYGDILSDIAAQVAGSVGLAGSANIGDRCAMFEAIHGSAPRRAGQNLA
                     NPSGLLLGAVMMLVHIDQPDVATRVHNAWLRTIEDGIHTYDIYHDGVSKQKVGTREFT
                     DAVIARMGMAPQQLK"
BASE COUNT          908 a         1452 c         1215 g          829 t
ORIGIN      
        1 aaaaaagaga gaggaacgaa ttcatgcctg aatatgtctg ggggcgcaac cctgtgttgg
       61 agaccctgcg ctcgccccgg cgcgtcaaac gtatcctgct ggcggatggg cagcgcgaat
      121 cgccggtcat ggtcgccatc gctcaagagg cggagcggcg gcacattccc atcgagacca
      181 cgccgcgcca gcgcctggat cacatcagcc agggggccgt ccaccagggc tgcatcgcgg
      241 tcgtcgaggc gcgcgcctac gccgacctgg atgatattct agccttcgcg caacaaaaac
      301 aggaagaccc cttcctgctg atcctcgacg ccatccagga tgtcaacaac ctcggatcgc
      361 tgctgcgcag cgcagaggct gccggcgcgc atggaatcgt catccccctg cacgaagccg
      421 ccgatgtcaa tgccaccgtt gtcaagacct cggctggcgc gagcgaacac ctgctgatcg
      481 cccaggaaac gaacctgacg cgcgtcatcg aatacctcaa gaagcaaaac gtctgggtca
      541 tcggcctcgc cggtgaagcc agcacgttct acgaccaggc cgacctgacc ggcccgctcg
      601 ccctggtcgt cggcaacgaa ggcaagggca tcagccgcct ggtgcgcgaa cactgcgacc
      661 tgctcatcaa actccccatg cacggcgtca tcaactcgct caatgccgcg gtcgccggca
      721 gcatcgccct ctacgaagcg ctgcgccaac gagagaagaa agcatcaaag tgacctggct
      781 acccgccgga atcgacaatc tcgcgcatct tcgctcgaat cgcctggccc gcttgccgtg
      841 tcgtggacgt gcctcccagg tcgggcgtca atacctgccc gcctgccagc gtctcttcga
      901 tggcgcgcat cagcgtcgcg gcggcctcgc gttctcccag gaaatcgagc aggagttgtc
      961 ccgaccagat ggatgcgacg ggattggcga tgcccttgcc ggtgatatcg ggcgcggaac
     1021 catgaattgc ctggaacagg ctggggtagg atcgttcggg attaaggttc gcgcttggcg
     1081 ccaggccgag gctgcccgtg accgccgcgc ccaggtccgt cagaatatcg ccgaaaaggt
     1141 tcgaggccac cacaatgtcc aggctctccg gcctggagac gaagcgcgcg gccagtgaat
     1201 ccaccagttg ttgttcatac ataatttccg ggtaatcagg cgctacctgc cggtagacat
     1261 catcccagaa aaccatgttg tactgcatac tgttcgattt ggtcgcgctg gtcacttttt
     1321 tgcgtttatg ttgccgggca tattcaaagg cgtagcggat gatgcgttct gtccccttgc
     1381 gcgtaaaaac cgtcgattgg atcgccactt cctcctcagc accttgatgc acacggcctc
     1441 ctaccccggc atattcgccc tccgtattct cgcgcacaca cactatatcg atctccccgg
     1501 ctcgcttgcc cgctaaggga ctcctgacgc ccggcagcag gcgcataggc cgcaggttaa
     1561 cgtactggtc gaacccctgg cgaataggca gcagcaggcc ccacagcgta atgtggtcgg
     1621 gtacacgcgg gtcgccgatg ggtcccatca ggataccgtc gaagttgcca cgttccaaca
     1681 ccctgagccc atcggtcggc atcaagcttc cctcgcgtag gtagcgttcc gtcccccaat
     1741 cgaactcctg aatatcaaaa cgaaagccgc catgcaggtc agccaggttc atcagcgttt
     1801 gcgtactctc actaataacc tccggcccaa taccatcgcc cggcagcgct gccagtcggt
     1861 atgatctcat cttttcgcct cgttttaacc ttctataacc ttttgcaggt cgttatctta
     1921 ctcccccata atcgaccgta gcctggcctt ccaatcatcc gcgccgccgt ctccctcgtc
     1981 gccatcctgt gtttcagcat ttccacccac cgtccagctg cgcgagccgt cggcgcggcg
     2041 cacaggcgcg gcccgttgga tcttcggctc ctttggttcc gtcgcgcgca aggcgatgtg
     2101 cgccgctttg cgcacatcaa atacggggtc ttgcagcagt gggaggatgg cgtccttgta
     2161 gcgttctccg tcaagccggc tcaatgcccg cacggcggcg gctcgctcat cgaaggtgga
     2221 cgcctgtaag atcggctcga tggcaggagc catcgcggta aaattcaggt cggtaattaa
     2281 tcgtagcagg cgactacgca gcatcgggtg aaccaggccc gccagcgccg cctcgcaatc
     2341 gcgcagcgcc tgctcctggc tggcaaccgt tccagccagg atcgcctcgg ccagtgctac
     2401 atccggcgac atgggcgaat gcccgcgcag gtgcggctcg atccacgccg ccaccggcgc
     2461 ttccaggttt acgctgtcat gcaggcgcaa gaaagttgca taatgccgct gcagccagtt
     2521 gtacagcgcc agcgtcattt ccgcgtcgcg cgcggcatag gcgatcatcg cagacggcag
     2581 cgggcggcgc gtccagtcgg tgcgctgcag gctcttgtcc agccgcacgt tgaaagcgcg
     2641 tagcagcatc gcagccaacg aggattcgcg ctggccaaat atcgaacggc tggccgcttc
     2701 caggtcatag tagtgtgcca ccaccagccc ccgttcggac atcacgcgca tgtcggcccc
     2761 cgcgccgtgc aacaggatac tgatcgccgg attgccgacg acgctcgcca gcagggacag
     2821 atcatgcacc cgcagcgcgt cgaccacaaa acattgcccc tcaatcgcca gttgcagcag
     2881 cgccaggcgc ggcccgctgc tcgctgctgt cccctgggca tgagagcgta tttgcgcaaa
     2941 ttcagcatcg atggcgatca cctgcgcctg ccgcagcgcg tccgcggcca tcgcaagctg
     3001 ctctgggcga tcaacccacg cgcgcgtccc atgccctccc tgtcctccaa actccggcag
     3061 cctctctacc ggtggcgaga ctcccaggta gccagccgca atctcatcgg caggctcctg
     3121 gccctcgcct ggctcagatc tcgtatcacg ttcattccta ctcaacagcg aaactttctc
     3181 atttaagatg tggcgtgtat tcttaccagc aactatatca gattttgggg caaatggata
     3241 gcatagccgc tagcgagcat ggcgactttg ccttgctccc cttgtacgaa ccgtgttcgc
     3301 tatgctaaac tgaagcagta ttttcacgtt ctaacgaaag aggacaaggt accactatgg
     3361 caaacaacgt tccaatcaca gttgcatacg gggatggcat cgggccagaa atcatggacg
     3421 ccaccctgcg tattatcacc gaagcaggcg ctcgcataga aaccgaagtg atcgaggttg
     3481 gcgaaaaggt gtatttgaaa ggcatcagcg cgggcatcga accgagtgcg tgggagtcac
     3541 tgcgccgcac aaaagtcttt ctcaaagcgc cgatcactac cccgcagggc ggaggttaca
     3601 agagcctgaa cgtcactacg cgcaccacct ttggtctgta cgccaacgtg cgcccctgcg
     3661 tctcctacta tcccttcgtc gcaaccaagc atcccacgat ggacgtggtt atcatgcgcg
     3721 agaacgagga agacctctac accggcatcg aatatcgcca gacccacgat atggtggaga
     3781 gcctcaagct catcagccgg cccggaagcg aaaagatcgt gcgctacgcc ttcgagtacg
     3841 cccgcagcca caaccgcaaa aaagtcacct gcttcatgaa ggataacatc atgaagctaa
     3901 ccgacggcct gttccacaaa gtcttcaatg agatcgcgaa cgattacccc gacatcgaaa
     3961 acgaggcatg gatcgtggat atcggctcgg cgaagctggc ggatacgccg gaagccttcg
     4021 acgtgatcgt actgcccaat ctgtatggcg acattctctc cgacatagca gcccaggtcg
     4081 ctggctcggt cggcctggca ggttcggcca atatcggcga ccgctgcgcc atgttcgagg
     4141 ccatccacgg ctccgctccc cggcgcgcag gccagaacct ggcgaatcca tccggtctgc
     4201 tgctcggcgc cgtcatgatg ctcgtgcata tcgaccagcc cgatgtcgcc acgcgcgtac
     4261 ataacgcctg gctgcgcacc atcgaagacg gcattcacac ctacgatatc taccacgatg
     4321 gcgtcagcaa gcagaaggtc ggcacacgcg aattcaccga cgctgtcatc gcccgcatgg
     4381 gcatggcgcc gcagcagctc aaag
//
LOCUS       DASZAK010000757         4586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_375566, whole genome shotgun sequence.
ACCESSION   DASZAK010000757 DASZAK010000000
VERSION     DASZAK010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4586
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375566"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VGT44_13300"
     CDS             complement(<1..1071)
                     /locus_tag="VGT44_13300"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581824.1"
                     /translation="MRTIAILDSTLREGEQFAGAYFTDEQRLTIARMLDAVGVGFIEI
                     PSPVASVETRRLTHALCDMELRAHIVAHVRCVEADVQAALDTPVYGLNLFYGTSPELR
                     AYSHGRRIEQMIADAVPLIRRIRASGRYTRFSAEDAFRSDLVDLLRVFDAVVEAGAHR
                     IGLPDTVGIATPRQVEQLVQLCQTRYPNVGIEFHGHNDTGCAIANTVAALEAGADCLD
                     VTVLGIGERNGIASLSGLIAQLYAHHPDQLAACDLTRLAPLDQFVAECLNLPIPFNAP
                     ITAPGAFTHRAGVHTKAILHNPRSYEILDPADFGLLRLVDVGSRITGRHAVGHRAAQL
                     GLQLTGQEVAELTGALRERAEQG"
     gene            complement(1221..3608)
                     /locus_tag="VGT44_13305"
     CDS             complement(1221..3608)
                     /locus_tag="VGT44_13305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-lysine hydrolase"
                     /protein_id="HEV2581825.1"
                     /translation="MSGIIDLENAHTSGVYGKRPIAIVRGQGALVWDDEGREYIDCTA
                     GYGIVNIGHARPEIAAAIAAQAQRLISCPELFYNDTRARLLERLARLTPQGLDHIFLC
                     NSGTEAVEGAIKFARLATGRTGIVATLRGFHGRTMGALSATWEPHYREPFAPLLPGVS
                     HVRYNDLAAMEAAITGETAAVIIELVQGEGGVHVATVEYVKGLAQLCRERGALLIVDE
                     VQTGFGRTGRLFACEHYALQPDILCLAKSLGGGVPVGAVCLGPRVMESGCITRGVHGS
                     SFGGNPLACAAALAALDIIEQEDLPARAASLGTRALERLRSLRAPLVREARGQGLLLG
                     IELTRRAQPFIDALGERGILALQAGPNVIRLLPPLVITEAQLDHALDIVEEVLTAQGN
                     GNVVAPTRSAIPHAPATTPLDQQSELSLLRQMLTIPSYSRQERALAHFLVEQARQMGM
                     DACVDEAGNFVASTHPRAEQGQPIVLLGHMDTVRGVVPVRLEDERLYGRGAVDAKGPL
                     AAFLCAAARLLSRVESHDQPVVVIGAVEEEAATSRGARAVVGRYRPAACIIGEPSGSQ
                     AITIGYKGRLLLDYRLARPASHSAGQQQNSSEAAIAFWNRVTRHAEEWNAQHSTQSSP
                     FASLMPSLRSITNSQDGLEEQTRLTLGYRLPPDFDTAALRTQLAQWAEEDQATLAFSG
                     EEATFQSTRATPLARAFVNAIRANGGQPSFKLKTGTSDMNVVGPIWGQNIVAYGPGDS
                     HLDHTPQEHIVVAEYHQAIDILEQVLIDLSHRDCHPERSEGSRSMANSDPSLRSG"
     gene            complement(3611..4456)
                     /locus_tag="VGT44_13310"
     CDS             complement(3611..4456)
                     /locus_tag="VGT44_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HEV2581826.1"
                     /translation="MTLVVKIGGGAGIATDTVLREIAQAVRDGERTVLLHGGSDAANT
                     LGERLGHPARMITSPGGMVSRYTDAQTLEIFAMAVAGQINTTLVAGLQQLGVNALGLA
                     GVDGRLLLARRKAVVRVVTADGRALILRDDYTGQVEQVNSVLLATLLDAGYTPVIAPL
                     ALSREGERLNVDGDRAAAAVAAALHADALVIITNVPGLLADPADHATLIRSIPAGLLD
                     LYMSYAQGRMRKKLLGAQEALQGGVPRVCIGSVSFLDVLNGAGTTIVRAGDEVVAGTL
                     SDERN"
BASE COUNT          840 a         1445 c         1486 g          815 t
ORIGIN      
        1 gccctgctca gcccgctccc gcagcgcgcc agtcaactcg gcgacctcct ggccggtcaa
       61 ttgcagccca agctgcgcgg cgcgatgccc gacggcgtga cgaccggtga tgcgcgagcc
      121 gacatcgacg aggcgcagca ggccgaaatc agccgggtcg aggatttcat acgagcgcgg
      181 attgtgcaga atggctttgg tatgcacgcc ggcgcggtgc gtgaacgcgc ccggcgcggt
      241 gatcggcgcg ttaaacggga taggcaggtt gaggcattcc gccacgaact gatcgagcgg
      301 ggcaagccgc gtgagatcgc aggctgccag ctggtcggga tgatgcgcgt agagctgcgc
      361 gatcagcccg ctcaacgagg caatgccgtt gcgctcgcca atgcccagta cggtgacatc
      421 caggcagtcg gccccggctt ccagcgctgc aacggtgtta gcaatggcgc agccggtatc
      481 gttgtggccg tgaaattcga taccgacgtt cggatagcgt gtttgacaga gctgtacgag
      541 ctgctccacc tggcgcggcg tggcaatgcc aacggtatcc ggcaggccga tgcgatgcgc
      601 gcccgcctcg acaacggcat cgaagacgcg cagcaaatca accaggtcgc tgcgaaaggc
      661 atcctcggcg gaaaagcgcg tgtagcggcc actggcgcgg atgcgccgga tcagcggcac
      721 ggcatcagcg atcatctgct caatgcggcg gccatgactg tacgcgcgca attctggcga
      781 cgtgccatag aagagattca ggccgtagac cggcgtatca agcgcggcct gcacatcggc
      841 ctcgacgcag cgcacatgcg ccacaatatg cgcgcgcaac tccatgtcgc agagcgcgtg
      901 agtcaggcgg cgcgtctcca ctgaggcaac cggcgagggt atctcgataa aacctacgcc
      961 gactgcgtcc agcatccgtg cgatggtcag gcgctgctcg tccgtgaagt aggctcccgc
     1021 gaattgctcc ccctcgcgga gagtcgagtc aagtatagcg atagttctca tgatttgtcc
     1081 cttctgaatg tcatgccaca tggccacaat gcacctgtag gagccgattt atcgagctcg
     1141 cagccgattt atcggcctgt gcggttcatt gatggcggat tcgcgccgat aaagattttt
     1201 accgatcaag tccgggactg tcatcctgag cgcagcgaag gatctgaatt cgccatggac
     1261 cgagatcctt cgctgcgctc aggatgacag tctcgatgtg acaggtcgat gaggacctgt
     1321 tcaagtatgt cgatggcctg gtgatattct gcgacgacga tatgctcctg cggcgtatgg
     1381 tcgagatgcg agtcgccggg gccgtaggcg acgatgttct gaccccagat ggggccgaca
     1441 acgttcatat ccgatgtgcc tgttttcagt ttgaaggagg gctgcccgcc gttggcgcga
     1501 atggcgttga cgaaggcgcg agccaggggc gtcgcgcgcg tggactggaa ggtcgcctct
     1561 tcgcccgaaa aagcgagcgt ggcctgatcc tcttcagccc attgtgccag ttgcgtgcgc
     1621 agggctgccg tatcgaagtc cggcggcagg cgatagccaa gggtgaggcg cgtctgctcc
     1681 tctaatccat cctggctgtt ggtgatgctg cgaagtgacg gcatcagtga ggcaaaggga
     1741 gaagattgcg tagagtgttg cgcgttccac tcttcggcat ggcgcgtgac gcggttccag
     1801 aaggcgatgg ccgcctcgct gctattctgc tgctgaccgg cgctatggct ggccgggcgc
     1861 gccaggcgat aatccagcaa tagccgccct ttgtagccga tggtgatggc ctggctgccg
     1921 ctcggttcgc cgatgatgca ggccgcgggc cggtagcgcc ccacaacagc gcgcgcgccg
     1981 cgcgaggtgg cggcttcttc ttccacagcg ccgatcacaa cgaccggctg gtcgtggctc
     2041 tctacgcgag aaagcaggcg agccgctgcg cacaggaagg ccgccagcgg ccccttcgcg
     2101 tcgaccgcgc cccggccata cagccgctca tcttccagtc gcaccggcac gacaccgcgc
     2161 actgtatcca tgtggccgag cagcacaatc ggctgccctt gctcagcgcg aggatgcgtg
     2221 ctggcgacga agttgccggc ctcgtccacg caggcatcca tacccatctg ccgcgcctgt
     2281 tcgaccagga aatgagccag cgcacgctcc tgccgcgaat aactggggat agtcagcatc
     2341 tggcggagca gggaaagctc ggattgctga tcgagcggcg ttgtggcagg cgcgtggggg
     2401 atggcactcc ttgtgggtgc cactacattt ccgttgccct gtgcggtcaa tacctcctct
     2461 acaatgtcga gagcgtggtc gagttgggct tcggtgatga cgagcggggg aagcaggcga
     2521 atgacgtttg gaccagcttg aagggccagg atgccgcgtt cgccaagggc atcgataaag
     2581 ggctgagcgc ggcgggtcag ttcgatgccg agcagcaggc cctggccgcg cgcctcgcgc
     2641 acgagtggcg cgcgaaggga gcgcaggcgt tccagggcgc gcgtaccgag ggacgcggcg
     2701 cgggccggca ggtcctcttg ctcgatgatg tcaagcgcgg ccagggctgc agcgcaggcg
     2761 agcggattgc cgccgaagct gctgccatgc acgcccctgg tgatgcaccc gctctccatc
     2821 acgcgcggcc ccaggcagac cgccccaacc ggcacgccgc caccaagcga cttcgccagg
     2881 cagaggatat cgggctgcaa agcgtagtgt tcgcaggcga agaggcgacc cgtgcgcccg
     2941 aagcccgtct gcacctcgtc cacgatcagc agcgcgcctc gctcccggca gagctgcgcc
     3001 aaccccttca catactctac ggtcgcaaca tgcacgccgc cttcgccctg caccagttcg
     3061 ataatgacgg ctgccgtctc cccagtgatg gcggcttcca tcgccgcgag gtcgttgtag
     3121 cgcacatgcg agacgccggg aaggagcggc gcgaacggtt cgcggtagtg cggctcccac
     3181 gtggcggaaa gcgcgcccat cgtgcggcca tgaaagccgc gcagggtggc gacgatgcct
     3241 gtgcgcccgg ttgccagccg cgcgaatttg atcgcgccct caactgcttc agtgccgcta
     3301 ttacaaagga agatatgatc cagaccctgc ggtgtgagac gcgccaggcg ttcaagcaga
     3361 cgggcgcgcg tatcgttata gaacagttcg ggacaggata ttagacgctg cgcctgcgcc
     3421 gcgattgccg ccgcgatctc tggccgggcg tggccgatat tcacgatgcc atatcccgcc
     3481 gtgcagtcga tgtattcgcg tccctcgtca tcccagacca gcgctccctg gccgcgcaca
     3541 atcgcgatgg gccgcttgcc gtagacgccg ctggtgtgag cgttttcgag gtcgatgatg
     3601 ccactcatgg tcaattcctt tcatctgaca gggtgcctgc aacgacctcg tcgccagcgc
     3661 gcacaatcgt tgtacccgct ccgttcagaa cgtccaggaa cgagacgctg ccgatgcaga
     3721 cgcgcggcac accgccttgc agcgcctcct gcgcgccaag cagcttcttg cgcatccgtc
     3781 cctgggcata ggacatgtat agatcgagca atccggcggg gatcgagcga atgagggtcg
     3841 cgtgatccgc cgggtctgcc agcaggccgg gaacgttggt gatgatgacc agcgcgtcgg
     3901 cgtgaagcgc agccgcgacc gcagcagcag cgcggtcgcc atcgacgttg aggcgctcac
     3961 cttctcggct caaggccagc ggcgcaatga cgggggtata gccggcgtca agcagcgtgg
     4021 cgagcaggac gctattgacc tgttcgacct gcccggtata atcatcgcgc aggatgagcg
     4081 cgcggccatc ggctgtcacc acgcgcacga cggctttgcg cctggccagc agcagccgac
     4141 catcgactcc ggccaggcca agagcattca cgcccagctg ttgcaaaccg gccaccagcg
     4201 tcgtgttgat ctgccccgcg acggccatag cgaagatctc cagtgtttgc gcgtcggtat
     4261 agcggctgac catgccgccc ggcgaggtta tcatgcgcgc gggatgcccc aggcgttcgc
     4321 cgagcgtatt cgcggcatcg gagccgccgt gcaataggac ggtgcgttcg ccatccctga
     4381 ccgcctgcgc tatctcgcgc aggaccgtgt cggtggcaat gcccgcgccc ccgccaatct
     4441 tgacaaccaa cgtcattatt gtgtcccttc tctagtaata ccccgtaggc gccggttcac
     4501 cgtgcgcatc gccgatttat cggctcgtgg agcgcgctga caacccctca ggccgataat
     4561 gaacattaac aaattaagtt ggatat
//
LOCUS       DASZAK010000758         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_551382, whole genome shotgun sequence.
ACCESSION   DASZAK010000758 DASZAK010000000
VERSION     DASZAK010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551382"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2619
                     /locus_tag="VGT44_13315"
     CDS             <1..2619
                     /locus_tag="VGT44_13315"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2581827.1"
                     /translation="IAGEPGIGKTRLAEETEREALHGGWIVAWSHAYAQESGIPYRLW
                     SELLRNLIAQGAWNPESAGQARLYAPLLALLPELRELWRDIKEQEQDQVRLREAVYEL
                     LAAISAQSPLLIVLDDVQWADGSSCELIGYVARRLSNQSIALLGTCRETELAANATLH
                     SLLGHMQREQVVEYLHVQPLADAEIAALVSHLPGNVVQHIQAQAAGNPFFAEELALSL
                     HNQEGNQSSISALPKTITAALNARMQRLSASCQQLLERAAVLGGSFGLPLIYAMETTP
                     FDEDTMLDLLDEAIRSGILTEEGTGARIAYRFWHPLLATHLYTTLSATRRGRIHRRAA
                     EALQSVYAAREHGLREQAAAITDHLVRGGAEAGQIANFAELAANYAYALSAYPEAEQR
                     YRQAAQALEQAAFDGQVDALHLAFLLERLAECSMIQGHFKEARALFERVLATRAAHHK
                     DVADRDTAHEDALETQVQALLWCEIAWTWRFTGDQERAWKCSKRGEQVLQEARITPGF
                     ARARLHYQQSSLLWQEGRYEEARLAAMQALEVFEAARIAQTAATQQTLQQKPSRLVED
                     AAHLTRILRTILGDPVDQGRTYALLGALANATGDRAEALEHMNKALALYERYDRQREI
                     AHACNNIGHVYMKQARYQQARSSLQRAYQLAERIGDAPLMGVVLHNLGELALSPDQRE
                     PQVAELYFKRSLLMAEQIDDREYLSRWNADLAFALIERGDLSGAASPAARALSVARAM
                     HNTPCAGLALIALGSLRLAQARTCMEQHNEIVARTNLQRARGTLQHALTLRGLEVETR
                     TRGRIILAETLAQLGERESANAQAALAMEEARQYELGELVKRCEAVMKSINPIEKVPG
                     RPQGSPPHSPPLPPLQ"
BASE COUNT          536 a          851 c          834 g          434 t
ORIGIN      
        1 atcgcgggcg agcccggcat cggcaagacg cgcctggcgg aagagaccga acgcgaggcg
       61 ctgcatggtg gctggatcgt ggcctggagt cacgcctacg cacaggaaag cggcatcccc
      121 taccggctgt ggagcgaact gctgcgcaac ctgattgcgc aaggcgcctg gaacccggag
      181 agcgcggggc aagcgcggct gtatgcgccg ctgctggcgc tgcttccgga attgcgcgaa
      241 ctatggcgcg atatcaaaga gcaggaacag gaccaggtgc ggctgcgcga ggcggtctac
      301 gaactactgg cagccatcag cgcgcagtcg ccgctgctga tcgtgctgga tgatgtgcag
      361 tgggctgacg gcagcagttg cgaactgatc ggctacgtgg cgcgccgcct gagcaatcag
      421 tcaatcgctc tgctgggaac gtgccgtgaa actgagctgg ctgccaacgc cacgctgcac
      481 tccctgctgg gacatatgca gcgtgagcag gtggttgagt acctgcatgt gcagccgctc
      541 gctgatgccg agatcgccgc cctggtgtcg cacctgcccg gcaacgtcgt gcagcatatc
      601 caggcgcagg cggcgggcaa cccttttttc gcggaagaat tggcgctgtc gctgcacaat
      661 caagaaggca accagtcgag tatcagcgcg ctgccgaaaa ccatcacggc agcccttaac
      721 gcgcgaatgc agcggctcag cgcatcatgc caacagttgc tggaacgagc ggctgtgttg
      781 ggcggctcgt ttggcctccc ccttatctac gccatggaaa ccacgccgtt cgatgaggac
      841 accatgctgg acctgctgga cgaggccatc cggtccggca ttttgacgga agagggaaca
      901 ggcgcgcgta tcgcgtatcg cttctggcat cccctgctgg cgacccatct ctacaccact
      961 ctgtcggcga cacggcgcgg gcgtattcac cgtcgcgcag ccgaggcgct gcaatcggtc
     1021 tatgccgctc gtgagcatgg actgcgcgag caggccgccg ccattaccga ccacctggta
     1081 cgcggcggcg ctgaagccgg gcagatcgcc aatttcgcgg aactggcagc caactatgcc
     1141 tacgcactct ccgcttatcc agaggccgaa caacgctatc gccaggcggc acaggctctt
     1201 gagcaagcag ccttcgatgg tcaggttgac gcgctccacc ttgcgtttct gctcgaacgc
     1261 ctggcggaat gcagcatgat tcagggacat tttaaggagg cgcgcgcgct gtttgagcga
     1321 gtgctggcga ccagggcggc tcatcacaaa gatgtggccg acagggatac cgcgcatgaa
     1381 gacgccctgg aaacccaggt ccaggcgctg ctgtggtgtg agatcgcctg gacctggcgt
     1441 ttcaccggcg atcaggagcg cgcctggaag tgtagtaagc gcggcgagca ggtcttgcag
     1501 gaggcgcgta tcacgcccgg tttcgcgcgc gcgcggctgc attaccagca gagcagcctg
     1561 ctgtggcagg agggccgcta cgaagaggcg cgcctggcag cgatgcaggc cctggaggtc
     1621 tttgaagcag ccaggattgc acaaacggcc gcaactcagc agactttgca gcagaagcct
     1681 tcgaggttgg tcgaagatgc cgcgcacctg acacgtatcc ttcgcaccat cctcggcgat
     1741 cccgttgacc aggggcgcac gtatgccctg ctcggcgcgc tcgccaacgc taccggagat
     1801 cgcgccgaag ccctcgaaca catgaacaaa gcactggcgc tctacgagcg ctacgaccgc
     1861 cagcgtgaga tcgcccacgc ctgcaacaac atcggccatg tctatatgaa acaggcgcgc
     1921 taccagcagg cgcgttcctc gttacagcgt gcctatcaat tggccgagcg catcggcgat
     1981 gcgcccttga tgggtgtagt gctgcacaac ctgggagaac tggccctctc gcccgatcaa
     2041 cgcgaaccgc aggtggccga actctatttc aaacgcagcc tgctgatggc cgagcagatc
     2101 gacgaccgtg aatacctcag ccgctggaac gctgacctgg ccttcgccct gatcgaacga
     2161 ggcgacctta gcggggccgc gtcgcccgcc gcgcgcgcgc tgagcgttgc gcgcgccatg
     2221 cataacaccc cctgcgccgg cctggctctc atcgcgctcg gttcgctccg cctggcccag
     2281 gccagaacct gcatggaaca gcacaatgaa atagtagccc gtactaacct gcaacgcgca
     2341 cgtgggacgc tgcaacacgc cctcaccctg cgcggcctgg aagtcgaaac cagaaccagg
     2401 ggccgcatca tcctggccga aaccctcgcc caacttggtg aacgcgagtc ggccaacgcc
     2461 caggcagccc tggcaatgga agaggcgcgg caatacgagc tgggggagtt agtgaaacga
     2521 tgcgaggcgg tgatgaagtc gatcaacccg atagagaaag tcccagggcg accgcaaggg
     2581 tctccacccc actccccacc actcccgccc ttacaatgac tacgaagtcg ctccacgtag
     2641 cgtaagtgaa cgagg
//
LOCUS       DASZAK010000759         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_551411, whole genome shotgun sequence.
ACCESSION   DASZAK010000759 DASZAK010000000
VERSION     DASZAK010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551411"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..>2241
                     /locus_tag="VGT44_13320"
     CDS             172..>2241
                     /locus_tag="VGT44_13320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2581828.1"
                     /translation="MREQQGPVMNEQRPERFGNYDIIKPIDTGGMGEVYLARQRSAFG
                     RPVALKIIRSDLVHDTTARARFLREAEVSAHLKHEHILPLFEFGEDQGRLFLVTPYIE
                     GGTLARRLQSGPLPLEEARQLFVPLAQAVAYIHRRGVIHRDLKPSNILLDEEDGQVYV
                     RLIDFGIASVQGGLASAPLTASGNEVGTIAYMAPERLNGVAAPSNDIFSLGIILHQML
                     TGNLPMGDEIDDMPDALRQVVYRCIALRPEDRYATVEDVLKHFERACQLIVSPMRPNS
                     MRLSPMDSSTISRAINDAPQILSRPMTSQPKEIMLEATGQFPSTPPETGESAPPRAFS
                     PGDYSARTMAFKSSQGQPASFSPIAPIPATHLAPLESPAQATPPLRRRRRRSLLPLLS
                     ALMAVILLAIAFLAYYGIQTVTTTEVTVKFGPKIVALNERYPMTATFSTSSVDAANAI
                     IPLHGLKDSQAGSIPGTATGQVNCIFGSFGCQTGVSQEDVTRLANQERPVLQANLTQA
                     LNQQMGSAGGTKIGAITFNDTTISSNPAVGQATNTVTVNLTEQASVVYYVTADAQGVA
                     RQLLARQAQQQGANYVLVSSTVQVGLPKVTGIDAATGKVSLSVAAGGDMVYQFPASQL
                     RAIQNALKGRTIDAAKTYLALQPGVDPGTIGINFTQGSGATLPGDIQHIEIVPSNPSN
                     YPSIQLQA"
BASE COUNT          467 a          679 c          658 g          437 t
ORIGIN      
        1 tgacaagcgc cctggactag ctgcttacgt aaacgtcaat ggggttagtt cctacagggt
       61 tgggtgaata atcagaaggc gtcgtcgcat tgacgaattt gtggtaacat acgtctatta
      121 tattagaggc atttatggca attatcaggt ttccgaaagt acgtgagacg tatgagagag
      181 cagcaagggc cggtaatgaa tgagcagcgg cctgaacgct ttggcaatta tgacatcatc
      241 aagcctatcg acacgggcgg gatgggcgag gtctacctcg cgcgtcagcg ttccgccttt
      301 ggccgccccg tcgctctaaa gatcattcgt tcagacctcg tgcatgatac gacggcacgc
      361 gcgcgcttct tgcgtgaggc tgaagtcagc gcgcacctca agcatgagca tatcctgcct
      421 ttgttcgagt ttggcgaaga ccaggggcgc ctgttccttg tcacgcctta tatcgagggt
      481 ggcacactgg cgcgccggct gcaaagcggc cccctgccgc tggaagaagc gcggcagctg
      541 ttcgttcccc tggcgcaggc ggttgcctac attcaccggc gcggcgtgat ccatcgcgac
      601 ctcaagccca gcaatatcct gcttgacgaa gaagatgggc aggtctatgt gcgcttgatc
      661 gatttcggca tcgccagcgt gcagggaggg ctggccagcg cgcctttgac cgcttctggc
      721 aacgaggtcg gcacgattgc gtatatggcg cctgaacgct tgaatggggt ggcagcgccc
      781 agcaacgata tcttctcgct gggcatcatc ctgcaccaga tgctgaccgg caatctgccg
      841 atgggcgatg agatcgatga tatgcccgat gcgctgcgac aggtggtgta ccgctgtatc
      901 gccttgcgcc cggaagatcg atatgccacc gtcgaggatg tgctgaagca ctttgagcgc
      961 gcctgccagc tcattgtatc gccgatgcgc cccaactcga tgcgcctctc gccaatggat
     1021 agctctacta tcagccgtgc catcaacgac gcgccacaga tactatcccg accaatgaca
     1081 tctcagccga aagagatcat gctggaagcg accgggcaat ttccctcgac accgcctgag
     1141 acaggcgagt ctgcccctcc acgcgcgttc agccccggcg actatagcgc gcggactatg
     1201 gctttcaaat catcgcaggg acaacccgcg tcattctcac cgatagcacc gatcccggcg
     1261 acgcacctcg cgccgctcga gtcaccagcg caggctacgc ctcctctgcg tagaaggcgc
     1321 aggcggtcgc tgctaccttt gctctccgcc ttgatggcgg tcattctgct ggcgattgcc
     1381 ttccttgcgt actacggcat tcaaaccgtc acgacgacgg aggtgacggt caagtttggc
     1441 ccgaagatcg tggcgctgaa cgagaggtat ccgatgaccg ccaccttctc cacgtcgagc
     1501 gtggatgcgg cgaatgcgat catccccctg catggcctga aagacagcca ggcaggttcg
     1561 atccccggca cagcgaccgg acaggtcaac tgcatctttg gctcctttgg ctgccaaacc
     1621 ggagtcagcc aggaagatgt tacgcgcctg gcgaaccagg aacgacctgt cctgcaggcg
     1681 aacctgaccc aggcgctcaa tcagcagatg ggcagcgctg gggggactaa aattggcgca
     1741 atcaccttca acgatactac gatctcctcg aaccccgccg tcggccaggc gaccaacacc
     1801 gtcacagtga atctgaccga gcaggccagc gtggtctact atgtcaccgc cgatgcgcag
     1861 ggcgtggcgc ggcaattgct ggcgcggcag gcgcagcagc agggggcaaa ttacgtgctg
     1921 gtgagcagta cggtgcaggt tggcctaccg aaggtgacgg gcattgacgc ggcgaccggc
     1981 aaggtcagcc tcagtgtcgc ggcgggcggc gatatggtct accagttccc ggcctcgcag
     2041 ctgcgcgcta tccagaacgc cttgaaaggc aggacgatcg atgccgcgaa gacctacctg
     2101 gctcttcaac cgggcgtcga tccaggcaca atcggcatca atttcacaca gggcagtggc
     2161 gctactctgc cgggtgacat tcagcacatc gagattgttc caagcaaccc cagcaattac
     2221 ccatcgatcc agttgcaagc c
//
LOCUS       DASZAK010000760         7726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_161918, whole genome shotgun sequence.
ACCESSION   DASZAK010000760 DASZAK010000000
VERSION     DASZAK010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161918"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VGT44_13325"
     CDS             <1..228
                     /locus_tag="VGT44_13325"
                     /inference="COORDINATES: protein motif:HMM:NF023075.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HEV2581829.1"
                     /translation="TLIRFNTANANVTGKNRQSRHYRGQCDYFAVYCEELNKTYLVPV
                     DQVGISRANLRIGPSKNNQEKNVRWAREYEL"
     gene            complement(230..302)
                     /locus_tag="VGT44_13330"
     tRNA            complement(230..302)
                     /locus_tag="VGT44_13330"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(267..269),aa:Ile,seq:cat)
     gene            complement(376..969)
                     /locus_tag="VGT44_13335"
     CDS             complement(376..969)
                     /locus_tag="VGT44_13335"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HEV2581830.1"
                     /translation="MAFSPDQEKTEVGQPDSADQPISPTAAISTAPLSVVLLQLPRLY
                     FNALVKPSLATYAEFKGQASWRLVWIQLATWAILDAALGVLVNTLYPPATGTAFSRFF
                     AFATTYGLVVVVPALFFLLMGIVYWIARAFGGKGTFLEQCHTSLIVEAPLGILSKLLA
                     LIPGIGRILNSVLSLYGIVLMVVVIIAVHRQSKGKIG"
     gene            1261..1587
                     /locus_tag="VGT44_13340"
     CDS             1261..1587
                     /locus_tag="VGT44_13340"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEV2581831.1"
                     /translation="MIQLLLVSATIALMIALVVYQVYKIRWLQAHGKRIVAVVISIKH
                     ETGKTAWGFPRDNYYVTAKWTNPRTGRTYTFWTWIMNSRPPFAKGSLVPIIIDPAHPE
                     RYALDL"
     gene            1808..3607
                     /locus_tag="VGT44_13345"
     CDS             1808..3607
                     /locus_tag="VGT44_13345"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEV2581832.1"
                     /translation="MENEITIRIGGESGEGTISGGDILALAAARWGYHVYTFRTFPAE
                     ILGGPCLFQVRISDSPVRSMGDYADVLVCLNKEAYDRNISDLRHGGVVVYDPSDFTLE
                     TDDYIAYPIPFNDLARKEVQMFQAKNMVMLGAISGLFGPPLQAISQVVESKLAKTRKS
                     NATLMEKNLLALDIASKYVTEQITRRDAYHLGAVEKADRVVLNGNQAVVAGALAAHCG
                     FFAGYPITPASDIMEGLAKELPANGGTFLQAEDEIAAITAIVGASFGGVRAMTATSGP
                     GFSLMSEAIGYASMAELPVVIVDAQRAGPSTGMPTKMEQSDLSFALNTSHGDTPRIVV
                     APANVADCYSLMIQSFNMAERYQMPVIFLTDQSLTARVESVERSAFMPMHVEERAWFN
                     ANGVTAANGHQGEAGNSTSNGQAAGEAAEHEHAFARYAYTASGISPISAPGAGAAPYV
                     ATGLEHDIYGHPNYEPEDHTAMMEKRYRKLITAAEELPSPQRYGDEDATVGLIGWGST
                     EGAIQEAVDRARAKGYKVASLHPRILSPLPDRAIHDFIRSVHTVIVPECNYTGQLANL
                     LGAKYGLQAIRVNKFGGIPFTAGEILRAIEEVS"
     gene            3610..4455
                     /locus_tag="VGT44_13350"
     CDS             3610..4455
                     /locus_tag="VGT44_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEV2581833.1"
                     /translation="MTQSTITLTPKDYKSEVKPTWCPGCGDFGVLNSIYNALSTLKLS
                     PDETVVVSGIGCSSRLPHFVQTFGIHSLHGRGLPLAQGLKMARPDLTVVAVGGDGDLF
                     SIGAGHLIHAALRNIDITVVAMDNEIYGLTKGQTSPTSPTGHTTKSTPYGLLASQFNP
                     IATALSLNVSFVARGFSSKPKELAAIVEQGIQHKGFSFIHVLSPCPTFYNTMDVWKTT
                     VTPIPADHDTHNRYKALELSMETQKPYVGIFYQEDRQTMDQAAHQQMQQAKTFDIEKY
                     MGRYS"
     gene            complement(4533..4760)
                     /locus_tag="VGT44_13355"
     CDS             complement(4533..4760)
                     /locus_tag="VGT44_13355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581834.1"
                     /translation="MTKNEFRQPVAVDTAPRGSKCEWCGKPAEVQLTAIGGHSHNDSG
                     IFCRACGEDFKRAVINSSALRTQGDARAQAV"
     gene            5174..5956
                     /locus_tag="VGT44_13360"
     CDS             5174..5956
                     /locus_tag="VGT44_13360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581835.1"
                     /translation="MDSSPRDTSIASDAHSLADQGEDELDEEAHPHIHLPNPSYWPLV
                     LGIALGLAFIGILIINSTPAVLIVALVFVLIGILGWALEDPMAPIKDIFVPVRVVVDP
                     WKYKIGQGVVDSQGNWLGKVRARFANYVLVERGGVRLKIFYVPVSAIGDQSKGNVLLL
                     TISEADLVRQGYNIVPDDLYEEMPEVGAPVIRGAPQFASRPLSPAETGHYNYGKHSPG
                     INTDAGHSYLRREINPIPQTYVTEEPIYVTDEPVPARAISPD"
     gene            complement(5977..7485)
                     /locus_tag="VGT44_13365"
     CDS             complement(5977..7485)
                     /locus_tag="VGT44_13365"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV2581836.1"
                     /translation="MPVLIILLIIAFILVILGIRKIYNLSSDPTAEKDFFPPVHHPGK
                     PLDEAPAQLPFSQFGGTINDVTGVNRTSIYGTLQIKSEDDLRNALQFARQHKRTVSIA
                     GARHSMGGQEFATDALVLDMRGFNQVTVDATSKMLTVQSGAIWHHILEYLNPYTLSIE
                     GMQSFDLPTVGGTISVNAHGLDRRIGGIASTIQSLRLMLADGSIRTLSRQENDELFQA
                     VIGGYGLFGIILDAQLSLMNNLAYKEVRSIIKTQDFPSAYTAIANDPTYHMFYARLSD
                     APSSFLKETIIYAYKVVDQILPLKPLTPENLVHLTRFVFNLGRKSYVGREIKWWAEKY
                     IQPLLETYPQSRNQILYRSYAYLKNNLQNNTDVLQEYFLPPEQVLAFIQGLGDILQRL
                     AVVTRNVEIRSVHKENILLDYAKADWLGVVLYLNFDVTEQGMRKIREAHSELIDLAQS
                     LGGSFYLPYQLSATRAQIDNSYPEFGKFLELKKQYDTDLLFTSEFYKTYGTT"
BASE COUNT         1868 a         2057 c         2014 g         1787 t
ORIGIN      
        1 acactgatca ggtttaacac agcaaacgcc aatgtcacgg gaaaaaatag acagtcgcgc
       61 cattatcgag gacaatgcga ctattttgcc gtgtattgtg aggaactcaa taagacctat
      121 ctcgttccgg tagatcaggt aggtattagc agggcaaacc ttcggatagg tccatcgaaa
      181 aacaatcagg agaagaacgt gcgctgggcg cgcgagtatg aattataaat gggcccagct
      241 gggatcgaac cagcgaccaa ctcgttatga gcaagctgct ctaaccgctg agctatgggc
      301 cctcgcggaa cgtattgtat catacctctc ttcctcttgg gaagagggta ttttgggatc
      361 aacctcctcc atcgctcaac cgatcttgcc tttgctctga cgatgtactg cgataatgac
      421 gaccaccatc aggacgatac cgtagaggct taatacggag ttgagaattc tgccgatgcc
      481 ggggatcagc gccaggagtt tgctgaggat gccgaggggc gcctcaacga tgagcgaggt
      541 gtgacactgt tccagaaagg ttcccttgcc gccgaaggcc cgcgcgatcc agtacacgat
      601 gcccatcagc aaaaagaaca gcgccggtac gaccacaacc aggccatagg tggttgcaaa
      661 cgcaaagaat cgcgagaaag ccgtgccggt tgcgggtggg tagagtgtat ttaccagtac
      721 gcctagggca gcatccagaa ttgcccacgt cgcgagttgg atccagacca ggcgccagct
      781 ggcctgacct ttgaactccg catacgttgc gagggatggt ttgacgagtg cattgaaata
      841 cagacgaggg agttggagaa ggacgacgct cagcggagcg gtagagatag cggctgttgg
      901 tgaaatgggt tgatctgcgg aatcgggttg accgacctcg gtcttttcct gatctggact
      961 aaaagccatg gcgtgttccc tttctacagc gagtcctttt ctagcgtgta tctatataga
     1021 catcttaatc gacaggtatc gcgtgcatcc taacatgacc aggtaagatg gtcaagcaag
     1081 ataggaaaat gtcctatgct gcttaccagg gcgatgctgg ctattgctct ggtataaccc
     1141 gctaaagcta cttgacgggt tactctcctg tgctgtataa tataactgtc aaattaaagt
     1201 tagagagtta gatatatcgc tgattatcag ttgcaagcgc ctttgaaagg agaagtcact
     1261 atgatacaac tactacttgt ttcggcaaca atagccctga tgattgcgct cgtagtctac
     1321 caggtgtaca agatcaggtg gcttcaagcc cacggaaaac gaatcgttgc cgtagtgatc
     1381 tcaatcaagc atgaaacggg caagacggca tggggctttc cacgggacaa ctattatgtc
     1441 accgcgaaat ggacgaatcc gcgcaccggt cgaacgtata cattctggac atggatcatg
     1501 aattctcgtc cgccgtttgc gaaagggagc ctggttccaa tcataatcga tcccgcacac
     1561 ccggagcgat atgctctgga cctctaggga agtttgacca ggctgaccgc caggttcgcg
     1621 tcagcctggg tgctcaattt tccatgctga cacgaaattc gtgctatcaa aactttcgca
     1681 atagagatta tgtgatataa tggctcagtg tcagagcaaa tgtacatacg ccattatgtt
     1741 gcaaacgggc cgcgccatac atttctgctt gtggcgcggc tatacattta tgttcagggg
     1801 gcattcaatg gagaatgaaa taacgatacg aattggcggt gagtcgggcg aaggaaccat
     1861 ctcaggtggc gatatcctgg cgctggccgc cgcgcggtgg gggtatcacg tctatacctt
     1921 ccgcaccttt cccgccgaaa ttctcggtgg cccatgtttg tttcaagtcc gcatcagtga
     1981 ttcaccggtc agatcgatgg gcgattatgc cgacgtgctg gtctgcttga ataaagaagc
     2041 gtatgaccgt aacatcagcg acctgcggca cggcggcgta gtggtctatg atccctccga
     2101 ttttacgctg gagaccgacg actatatcgc ctatcccatc ccgttcaacg atctcgcgcg
     2161 caaagaagtc cagatgtttc aggccaagaa tatggtcatg ctgggcgcga tctccggtct
     2221 gttcggcccg ccgctgcagg ccatctccca ggtggtcgag tcgaaactcg cgaaaacgcg
     2281 caaatccaac gcgaccttga tggaaaagaa cttgctggct ctcgacatcg cctcgaaata
     2341 tgtcacggag cagattacca ggcgcgatgc gtaccatctc ggcgctgttg agaaagcaga
     2401 ccgagtggtg ctgaacggca atcaggcggt ggtcgcgggc gcgctggcgg cccactgcgg
     2461 cttcttcgcg ggctatccca tcacgccggc cagcgacatc atggaagggc tggcgaagga
     2521 actgcctgcg aacggcggca ccttcctaca agcggaagac gagatcgccg ctattaccgc
     2581 catcgtgggc gcatccttcg gcggtgtgcg cgccatgacc gccacatccg gcccaggctt
     2641 ctcgctgatg agcgaggcca tcggctatgc atcgatggcc gaactgccgg tcgtcatcgt
     2701 cgatgcccag cgcgccggcc ccagcacagg aatgccgacc aagatggagc agtccgatct
     2761 cagcttcgcg ctcaacacca gccatggcga cacgccgcgc atcgtcgtcg cgcctgctaa
     2821 cgtcgcggat tgctactcgc tgatgatcca gtcgttcaac atggccgaac gctaccagat
     2881 gcccgtcatc ttcctgaccg accagtccct gactgcccgc gtcgagagtg ttgagcgcag
     2941 cgccttcatg ccaatgcatg tcgaggagcg ggcgtggttc aatgccaatg gcgtaaccgc
     3001 cgcgaacgga catcaaggcg aagctggcaa ctcgacaagc aatggccagg ctgctggtga
     3061 agctgctgag catgaacacg ccttcgcgcg ttacgcctac accgccagtg gcatctcgcc
     3121 gatatcagcg ccgggcgcgg gtgcggcacc ctacgtggcg accggcctgg aacacgatat
     3181 ctacgggcat cccaactacg agccggaaga ccataccgcc atgatggaga agcgctatcg
     3241 caagctcatc acagcagccg aggagctgcc gtcaccacaa cgttacggcg acgaggacgc
     3301 cacggtcggc ctgatcggtt ggggttctac ggaaggcgcc atccaggaag cggtagaccg
     3361 cgctcgagcg aagggctaca aggtagcctc gctgcacccg cgtatcctct cacctctgcc
     3421 ggaccgcgcg atacatgact ttatccgctc agtccacacc gttatcgtgc ctgagtgcaa
     3481 ctataccggg cagcttgcca atttgctcgg cgcgaaatat ggcctgcagg ctatccgcgt
     3541 caacaagttc ggcgggatac cctttaccgc cggtgaaatt ctgcgggcta tcgaggaggt
     3601 tagctaaacg tgactcagtc aacgatcaca ttgacaccaa aggattacaa gagcgaagtc
     3661 aagccaacct ggtgtcctgg ctgcggtgac ttcggggtgc tgaactccat ctacaacgcc
     3721 ctgtctacct tgaaactctc gcctgacgag acggtcgttg tttcgggcat tggctgttcg
     3781 agccgcctgc cgcactttgt gcagaccttc ggcatccata gcctgcacgg gcgcgggctg
     3841 cccctggcgc aaggcttgaa gatggcgcgc cccgatctga cggttgtggc ggtcggcggc
     3901 gatggcgacc tattcagcat tggcgcgggc cacctgatcc acgctgcctt gcgcaacatc
     3961 gacatcacag ttgttgccat ggataacgag atctacggcc tgacgaaagg tcagacctcg
     4021 cccacatctc ctaccgggca caccaccaaa tcgacgccgt atggcttgct cgcctcgcag
     4081 ttcaacccga ttgccaccgc gctctcgctc aatgtcagct tcgtggcgcg cggcttctcg
     4141 tcgaagccga aggaactggc cgcgatagtc gagcagggta tccagcataa aggcttctcg
     4201 ttcatccacg tcctcagccc atgcccgact ttctataaca cgatggatgt atggaagacg
     4261 acggtcacac caattcccgc cgaccacgat acgcacaatc gctacaaggc cctggaactc
     4321 tcgatggaga cgcagaaacc ctacgttggc attttctacc aggaagaccg ccagaccatg
     4381 gaccaggccg cgcatcaaca aatgcagcag gccaaaacct tcgatatcga aaagtatatg
     4441 ggacgctatt cctgagcgaa ctggcataag caaagggcgt gggtaaacgc ggttgaccgc
     4501 gtttacccac gccctttgct tctattcaat gattagacgg cctgcgcgcg ggcatcgccc
     4561 tgggttcgca gcgcagagga gttgattacg gcgcgcttga agtcttcgcc gcaggcgcgg
     4621 cagaagatgc cgctgtcatt atgcgaatga ccgccgatag cggtcaactg cacctcggca
     4681 ggtttgccgc accactcgca cttgctccca cgcggggcag tgtctacagc aacaggctga
     4741 cggaattcat ttttggtcat caggtatcct cctggttcca gtttcttccc ggtttcacac
     4801 aggaacaaac gttaggggaa acccgtatct tgtggttaac aactcggaga agtccacgat
     4861 gatacggcat atacatatac gcgcgtcatc gcccaggagc gtcgctgctc ccctgttcag
     4921 cattgcggag aaccagactg atggatgaag gtaggtaaat tctgctatcc tactaagaat
     4981 actatagcac attttggcaa attatgccaa taaaacgcgg atgtgttcag ggcgatggcc
     5041 atcgccctga acacatccgc gcaacaacct gcgcttgcac aatcttcaca tttcttgtat
     5101 gcttgtatta agaacgattg tacgcctggc gtacttcgta caacatcaaa gacctggaag
     5161 gaactgagaa cgtatggata gttctcctcg tgatacaagt attgcatccg acgctcactc
     5221 cctcgccgat caaggggaag acgaactgga cgaagaagcc catccgcata ttcacctgcc
     5281 caatcccagc tactggcctc ttgtgcttgg tatagcgttg gggctggcct tcatcgggat
     5341 actaattatc aatagcaccc ccgccgtgtt gatcgtggcg cttgtgtttg tgctcatcgg
     5401 catcctggga tgggccttag aagacccgat ggctccgata aaggacatct ttgtgccagt
     5461 tcgcgtcgtc gtcgacccct ggaagtataa aatcggccag ggcgtcgtcg actcccaggg
     5521 gaactggctc ggtaaggtgc gcgcgcgttt cgccaactat gtgctggtcg aacgtggcgg
     5581 cgtaagactc aagatcttct atgtccctgt cagcgccata ggcgatcaga gcaaaggcaa
     5641 tgttctgtta ctcaccataa gtgaagctga ccttgtgcgc cagggctata acattgttcc
     5701 cgatgatttg tatgaggaga tgcctgaggt aggagcgcca gtcatcagag gcgcgccgca
     5761 attcgcgagc agaccgcttt cgcccgccga aaccggccac tacaactacg gcaagcactc
     5821 gccaggcatc aataccgatg caggtcattc ttatctacgc cgcgagatca atccgatccc
     5881 gcagacctac gtgactgagg aaccgattta cgtgacggat gaaccggttc ccgcgcgcgc
     5941 gatttctcca gattaaagtg gggctggcat tgtcaactac gtcgttccat acgttttata
     6001 aaactcactt gtaaatagga ggtcagtatc atattgcttt ttgagttcta aaaatttgcc
     6061 gaattcaggg taagagttgt ctatttgcgc tcttgtggca ctgagctggt acggcaaata
     6121 aaagcttcca cctaagctct gggccagatc gataagttca gaatgtgcct ctcttatttt
     6181 tctcataccc tgctcagtca cgtcaaaatt taagtagaga acgacaccca gccaatccgc
     6241 tttggcgtaa tcaagcagaa tattttcctt atgaactgat cttatctcga cattgcgcgt
     6301 tacaaccgcg agtctttgca aaatgtcacc taatccctgg ataaatgcca ggacctgttc
     6361 tgggggaagg aaatattctt gtaaaacatc cgtgttgttc tgcaggttat tttttagata
     6421 agcataagaa cgatacagga tttgatttct tgattgaggg tacgtttcca gaagtggttg
     6481 aatatatttt tcggcccacc attttatttc ccgacccaca taacttttac gccccagatt
     6541 aaagacaaag cgtgtgagat gcaccaaatt ttcaggcgtg agcggtttta gaggtagtat
     6601 ctgatcaaca actttataag cgtaaataat cgtttctttg aggaacgagg aaggggcatc
     6661 ggataaacgg gcatagaaca tatgataggt cggatcattc gcaattgctg tatacgccga
     6721 gggaaaatcc tgtgttttaa taatgctccg cacttcttta taggcaaggt tattcatcaa
     6781 gcttaattgc gcatccaaaa tgataccgaa cagaccatat ccacctataa ccgcttgaaa
     6841 aagctcatca ttttcttgcc tgctcaatgt tctaatactg ccatccgcta gcatcagacg
     6901 tagcgactgt atggtcgatg caattccgcc aatgcgacga tccaggccat gtgcatttac
     6961 ggagatagtc ccgccaacag taggcaaatc aaaagattgc attccttcaa tcgagagggt
     7021 gtagggattt aaatattcaa gaatatgatg ccaaattgcc ccgctttgta ccgtcagcat
     7081 tttgcttgtt gcatcaacag tcacctggtt aaatccgcgc atatccagga cgagtgcgtc
     7141 tgttgcaaat tcctggcctc ccatactgtg cctggccccg gcaattgaaa cagtgcgctt
     7201 gtgctgtcgc gcaaactgca gtgcattcct caggtcatcc tcagatttta tttgtagggt
     7261 gccataaatc gaagttctat tgactccagt gacatcattg attgttccgc caaattgcga
     7321 aaaaggcagt tgcgccgggg cttcatccag tggtttgcct gggtgatgga caggaggaaa
     7381 gaaatctttc tcagctgtag gatcagaaga gaggttatat atttttctaa tgcctagaat
     7441 caccaatata aaagcgatga ttagcagaat aatcaaaact ggcataagtt tcgcaactcc
     7501 tgtttttcag tacaattcga atacttcgaa aaaatgggtt tccgggtttt gttagagccg
     7561 gagtccgctg atctatcagc atttatacag agcgttgttg tgattgcgat ttcaccacgg
     7621 tcttatagta atcgcacaca tcggtgatcg gacatttgtt acatcgcggt cgctcaaata
     7681 cgcatatttg ctgaccatgc cgcaataacc ccttgtggaa attgta
//
LOCUS       DASZAK010000761        14275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_195017, whole genome shotgun sequence.
ACCESSION   DASZAK010000761 DASZAK010000000
VERSION     DASZAK010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 14275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14275
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195017"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..1405)
                     /locus_tag="VGT44_13370"
     CDS             complement(98..1405)
                     /locus_tag="VGT44_13370"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV2581837.1"
                     /translation="MHNTKRWYIFAACIMILAIGAYYAGNAFSSLHAMAKAHPRTSST
                     TPIQHIVFMVKENRTFDSMFGTYPGANGATTWVDLSGVTHPLYHQPIQLKNDIKHSYV
                     TAVRAEDNGKMDRFQNIPGSSQLGIYASVSQLYQSDIPNYWAYAQNFALEDNFFSDES
                     GPSFPNHLFTIAGEDGNAADIPHGGGWGCDARSTTTVETIDPSDVTSYVFPCYDFQTL
                     GDLLDQAGISWKYYAPPKGNPGYIWSAYDAINHIRNGSDWTSHVVDYSQFAVDAAAGN
                     LPAVSWLVTPGNVSDHPPYNVCNGENFTVSQINAIMGNSSEWASTAVVLTWDEWGGFY
                     DHVYPPKGPNPYIEYGFRVPALIISPYARAGYIDNTFNSFSSMLKFAEDTFGLPSLTS
                     LDGNSNDLYSAFNFNQTPIPPLVLQTRTCPPAAAQPDNLVTTD"
     gene            complement(1669..2904)
                     /locus_tag="VGT44_13375"
     CDS             complement(1669..2904)
                     /locus_tag="VGT44_13375"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEV2581838.1"
                     /translation="MNRRKAFILLGVLAALLAIAAGSLVYFAIAGGGRGGQTTTNLTP
                     TQGSTGVNVTPIVGGTAHPGTGCGSVKNSQGTYTFSWLHVASDGKVVDENNCIVNLVG
                     FNMAPLFLGDAVGSISAPKVAWYKQTFPMNLVRINFNSTWWDQDVYVPDAGMHFRAWL
                     ELNVKWQEQVGNYIELDNGPNFTDPPCGGTVTFCPAQDQGKRDYQANPNPTTALELET
                     NIQPGVQAWTDLAKLYANDPAVVYDAWNEPNIKDIPTFFHDMNTLINTIRAQNPRALL
                     IVYERGLTNIQNGSEPAYQQPNIVFDAHIYPGFNGTSPIDGKHCHTPGSGNPVDAQLQ
                     QAVAYAHSHGEGFMIGEWGGCYDVPGYDSQLVSFAKANYLPLTYFAAGDVVVSEKATP
                     LALNGNGLLVKADYTSING"
     gene            complement(2936..4480)
                     /locus_tag="VGT44_13380"
     CDS             complement(2936..4480)
                     /locus_tag="VGT44_13380"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV2581839.1"
                     /translation="MRVFLFGVDGLTFRVLNPLMERGLLPNFSRLRAEGVEGILKSTT
                     PPVTPPAWTSIATGLAPAKHGVFDFWEYEQTASGPAAHVMTHRKGGRAIWNILSDWGK
                     QVIVANVPMTYPPEPVNGIMLSGLMSPDMKANVTYPVAFKEELLEQVPGYHIDLNAAV
                     SNGQVGEVLTDTLKMTQERNEMLNLLLKKPWDFFFIVYTGADRIQHMRWDEIMSFHPS
                     AVRYYEMVDEALGKVLDGLMPDDMLMVVSDHGFQGVTRKFYLQEYLYRKGWLQMNASE
                     RRGARLYGIARAIVRAVGLQRIALPLYRRLRHKQVNHMAETDHVAILPNIDWAKSRAW
                     IQSTSGELAGYADVYFDDTVTEEEIDEFIEALRAFRDPENGQPLALEIHREDAFGSGQ
                     LAPDQRHVIVLSGESTSIYTQLGRTSLWETRGVGRSVSTGIHHPDGVLYLYGSGVKKG
                     VTMTPAHVYDVVPTILSFMGVPPPDELDGKALVEPFERSDIGAQDVEHYDLVMRKLRR
                     LAEKTS"
     gene            complement(4482..6032)
                     /locus_tag="VGT44_13385"
     CDS             complement(4482..6032)
                     /locus_tag="VGT44_13385"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV2581840.1"
                     /translation="MTRVLLFGVDGLAFRILNPMIERGLLPNFQRVRDEGAQGILAST
                     VPPMTPPSWMSISTGVSLSKHGVYDFWEYEQTAEGPRAHVLTHRKGSKAIWNILSEWG
                     KRVVVANVPMTYPPEPVNGMMLSGYMAPDMRADVTYPGEFKQELLDAVPEYMIDLNPA
                     VSGGQVGDPFAETLKMTQERIAMFRLLLSKTWDFCFVAFVGADRIQHLRWEKILEFHP
                     QAVAYYQMLDEALGMALAELKSDDVLMIVSDHGFQGAKRTFYIQEYLYKRGLLRVRDG
                     GRRNQALFINSIKRFVQSLGLKKLARKTMRYLRRSGVVAVEEEYHAVKLPDLDWQRTQ
                     AWVPSSSGSMAGYADIFLAESLSVESICDLMRDLRAVRDPQTGQLLVQELYREEVYGE
                     GAHQPGERHLVVLASESCNLATDLGRQSLWETNAAATGIHHPDGVLYLYGANVKHGVS
                     LAPSQVYDVVPTILSRMGLPLPPELDGQVINEAFEQPSTAESPGEGLVLKKLKKLATS
                     GTTGKGDY"
     gene            complement(6072..7613)
                     /locus_tag="VGT44_13390"
     CDS             complement(6072..7613)
                     /locus_tag="VGT44_13390"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV2581841.1"
                     /translation="MRAVIFGVDGLSFRILHPLIQRGDLPNFARLQREGVEAEFISAV
                     PAITPPAWMSLVTGLKPAKHGVFDFWEFDTNSETLKSRAVTHRKGGKAVWDILSEYGK
                     RVIVMNVPLTYPPDPVNGIMVSGLMTPSTSSPFTFPHSFKDELYKAVPGYLVDLGSER
                     VTAGRFVDAVLDMTEKRIQLQEHLLSEHDWDFAFLSYVGPDRIQHRIWDSIESLSQEG
                     TKYFRLLDDALGRVLNRLTAEDVLFVASDHGFVGARKWFYINEYLCRRNLLQRDGNAE
                     SSRSKLIGFGREAAQKMHLLNLARKARKVYTDFSDEPMMEKSKSHYKPLFESMDWNAN
                     RACVLSLTAFGSGMADIFMSPKASAEDMEELRQALAAERHPETGELLAEAVYATDVYG
                     VGPFRPPEEHIVLVPASGTTFHLKRGRKQLWETLDQPKGVHEKEGVFYAWGAGMRQGK
                     RIEPLQIYDLVPTVLQAVGVSANEPFDGHVAQDVFQRVQNNTNDADDLVARKLKRLQV
                     SLPLQ"
     gene            complement(7652..8665)
                     /locus_tag="VGT44_13395"
     CDS             complement(7652..8665)
                     /locus_tag="VGT44_13395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2581842.1"
                     /translation="MKRPLVSIIIISCNRPLLLQHCLERVYDQPYPDKEVIVVDSSSN
                     DESEKVIARYPGTISVRLRGQRNNMPYARNEGMAVSSGDLIAFIDDDSMIFPLWLNAM
                     VDAYKDESIGAVGGRIIRRPEPYCDLEDGSPSLLVKPSGIAIARDFDLVSEAALEVDH
                     LVGCNMSFRRAALEQIGGFDSNYTLTNYREETDMFIRLKKAGWRIMFVPAMSVRHVSA
                     RSAKPFFMERPYIQFSNGRNSAYLAYKNFGFNFRTVTGQFLEIGAAFRRAGFLVGLGV
                     TGLAAQLGGRAVGMAAGVAWHVRKNASAGTQLNLQRPSRTASEPEIIAVASSEQLDSV
                     LSE"
     gene            complement(8713..9762)
                     /locus_tag="VGT44_13400"
     CDS             complement(8713..9762)
                     /locus_tag="VGT44_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581843.1"
                     /translation="MNYYQTLTGMGSGKFGRFDAVRPEKYGVVEDTGVPEGAMGRLLQ
                     DVLQARKLSTALLEAKSLDELGALFDQAYDCLIVRFAAGGLDTAGLESLVERFSQLAS
                     SDDHFFVLTDVWYPQPVNSVNVNDFLADRGILEVNEPRQRSGIIWSESLAYGVGTGQI
                     WINLQGREAEGSVRSGNEYQQVRDVLVNELSNNWRDPQTGEPVVAQVLRKEEAYSGAY
                     LFKSPDLVVVYRPGYVPSPKAAALDFDGASVGPRASASPVSAPAARLIGAGPRLAKGF
                     KGEASLVDVMPSIMYLLGQPIIMDVDGNVITSMFTPAYAEQTPIQRINSDDELLSDEE
                     EGMIVDRLRDLGYLG"
     gene            complement(10085..10951)
                     /locus_tag="VGT44_13405"
     CDS             complement(10085..10951)
                     /locus_tag="VGT44_13405"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HEV2581844.1"
                     /translation="MSRLTDEARKIDTYFRSSSSPWRVLPDFIIIGAQKGGTTSLYNY
                     LIEHPSIKSARRKEVHFFDRNFHKGLSWYRTFFPTALEKYYTERVLKREFITGEGSPE
                     YLFYPHCAQKAWQTLPQVKIIALLRNPVERAYSQYRHNLGWGHIKDMSFQDALALEEE
                     QTKEGKARAQVDEHYHDFAYQRASYLARGLYAEQLKKWMDLFPREQFLILRTEDFYSD
                     PAAIYTETLRFLGVSSLEPKGLKKGYRIYNQSSESTPSKMDPATRERLSAYFAPHNAQ
                     LYELVGRDFGWH"
     gene            complement(11013..12464)
                     /locus_tag="VGT44_13410"
     CDS             complement(11013..12464)
                     /locus_tag="VGT44_13410"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV2581845.1"
                     /translation="MTLIIYLVLNLALAYLMTINLALGLLGAVAINAVVFLFLRSRFA
                     LPLYIIVAGPSLSITLSQSGILSRLYIGNMLLALLAIIWLAQFVLPERKSGSKLLSRG
                     LLIPLLLINIVGLISIIYSRIYPDPNVPYTYPHSNVSITIVNLSEMALLLGLPLLLII
                     ARKMVRTVRDYKLAFRAFIVAGVLYTLGTIFAAPLGFYSKKVILGVRRVEVFGLDSSG
                     LGTTLVLFTDLAFGQALYSEGRRRLGWGLLTLLFAIGVILAFGREAWLELFMSMLVML
                     VFRTKNWAALLFLLLPIALLFVPGVSDFFNSSKVYGSDRLKIWADAITIWLKSPYFGI
                     GAGNYQFYDWIYGVDKAGLAHNQYLEVLAEMGVQGLIALLWMLASFGVIAFKSFKGAK
                     TRLSKSLTISFVGFYLCFIFGGFFTGILIPSAAAGGGTAGLIVASYHWLFLGLVITIP
                     NWEKEAIQEEQLLSPPQLDTLSPLNKVVVSHAE"
     gene            13149..>14275
                     /locus_tag="VGT44_13415"
     CDS             13149..>14275
                     /locus_tag="VGT44_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEV2581846.1"
                     /translation="MSTFHVEIPPEMLTTSPLFATVVERTETQPLQAFQQQAGVESRL
                     RNREFVPDTPDHEVYSVSQFLEHATIPLIASEATTNHAEPAALVTKSEISGTANNAAI
                     VGVGNIVGFLFKYGNNLLIQHALGVALFGLYSVSLSLVTLLGSIFDLGLDNTVIRYIS
                     VYRGKKQKSMMRHLLTFCTGMAAITGTLGAVLVLFFASSLANFVHKPDTIPLLQIMAP
                     VVPLLCMQTVWTSGLQGLKEFKRRVLIQRFVVPLTLFALTAIALFFFRNLTGVAILAF
                     CSTLISTIINLYFLFAAVNRIKGSEDGRYETRSWLSFALPNFLTTIVNTALDSIDTLL
                     LAFFVAANAIGLYAAAIKISNFILIPLATMNIVFTPTIAEL"
BASE COUNT         3366 a         3986 c         3615 g         3308 t
ORIGIN      
        1 cgccatgcct ctgcactggt aactacactc ccacctcaca gagaagtctc actagagacc
       61 cgacgcattg catcgaatgc gccgggtttt tgtttcatca atccgtcgtg actagattgt
      121 cgggctgcgc cgcggcaggc gggcaggtcc gcgtttgcaa tacaagaggc gggatgggtg
      181 tctggttgaa gttaaaggcg ctgtagaggt cgttggaatt cccatcaagg ctggtgagcg
      241 atggcaggcc gaacgtatcc tcagcgaact tcagcatcga cgagaagcta ttaaacgtat
      301 tgtcaatata gccggctcgc gcgtaggggg agatgatcag cgcaggcacc cgaaagccat
      361 actcgatgta cgggttgggt ccctttggcg gataaacatg gtcgtagaaa ccgccccact
      421 catcccacgt cagtacaacc gccgtactcg cccactccga ggagttgccc ataatggcat
      481 tgatctgcga tacggtgaag ttctctccgt tacagacgtt gtagggagga tggtcactga
      541 cgttccccgg cgtaaccagc cagctcaccg cgggcaggtt accggcagcg gcatcaacgg
      601 caaactgcga gtagtcgacg acgtggcttg tccaatctga cccgttgcgg atatggttaa
      661 tggcatcata cgccgaccag atgtaaccag ggttgccctt cgggggagcg tagtacttcc
      721 aggaaatgcc ggcctgatcg agcagatccc ccagggtctg gaagtcgtag caggggaaga
      781 catacgaagt gacatcgctc ggatcaatcg tttcgaccgt cgtcgtagag cgggcatcgc
      841 agccccaccc gccgccatgc gggatgtcag cagcattgcc atcttccccg gcaatcgtga
      901 aaaggtgatt ggggaagctc ggacccgact catccgagaa gaagttgtct tcgagggcga
      961 agttttgagc ataagcccaa tagttcggga tgtctgactg gtacaactgc gagactgatg
     1021 cgtatatccc cagttgggac gagccgggga tgttctggaa cctgtccatc ttcccattgt
     1081 cttccgcacg gaccgcggtg acgtatgaat gcttgatatc gtttttgagc tgtataggct
     1141 ggtgatacag gggatgcgtc actccactga gatcgaccca tgtcgttgct ccgttcgctc
     1201 ccggatacgt cccgaacata ctatcgaagg tcctgttctc cttgaccatg aagacaatat
     1261 gctggatcgg ggtcgtcgag cttgtgcggg ggtgcgcctt cgccatggcg tgcagggaac
     1321 tgaaggcatt accggcataa tatgcgccaa ttgccaaaat cattatacac gcggcgaaga
     1381 tataccatcg ttttgtatta tgcatgactt ttctcctcaa gtaggatatt tgagactggt
     1441 aaactgtccg atgactcgat ccgtaaccta tgtcttgcta ttatccggga ttggagcatg
     1501 aaagtagatt gaactgagat cgatgtacgt agtatacatg gaaaattaca taaatacaat
     1561 gttccatgtc gcctcattct gtatcttttt agttcaattt ttaaatcccc ccttcgattg
     1621 ttgagtggtt agcgtcgatt gcgcccgcca ggaattggca aggccttatc acccattaat
     1681 gcttgtatag tctgccttca ccagcagccc attgccgttg agggcgagag gggtggcttt
     1741 ctcgctaacc accacatcac cggctgcaaa gtatgtgagc ggcaggtaat tcgctttcgc
     1801 aaaagagacg agctggctat catagcccgg gacatcgtag catcctcccc attcaccaat
     1861 catgaaaccc tctccatggc tatgggcata ggcgacagcc tgttggagct gcgcatcgac
     1921 cgggttcccg ctgccggggg tgtggcaatg ttttccatcg ataggcgacg tgccattgaa
     1981 gccagggtaa atgtgcgcgt caaacacaat gttcggctgc tgataggccg gctctgaacc
     2041 gttctggata tttgtcagtc cgcgttcgta cacaatcaga agcgcacggg ggttctgcgc
     2101 cctgatcgta ttgatcagcg tgttcatatc gtggaagaag gtcggaatat ccttaatatt
     2161 tggctcattc caggcatcgt agacgaccgc agggtcattt gcgtacaatt tcgcaagatc
     2221 cgtccacgcc tgcacacccg gctggatgtt cgtttccagt tccagggcag tcgtgggatt
     2281 cggattggcc tggtagtctc tcttcccctg gtcctgcgca gggcaaaatg ttacggtgcc
     2341 gccgcaagga ggatcggtaa agttggggcc gttatccagc tcaatgtagt tgcccacctg
     2401 ctcctgccac ttgacattga gttcgagcca cgccctgaaa tgcatcccgg catcgggaac
     2461 ataaacatcc tgatcccacc aggtcgagtt aaagtttatg cgcaccaggt tcatcggaaa
     2521 cgtctgtttg taccaggcta cttttggggc gctgatcgaa ccgacggcat cccccaggaa
     2581 gagaggcgcc atattaaagc cgaccaggtt gacaatgcag ttgttttcat cgacgacttt
     2641 gccgtccgac gcgacgtgga gccaggaaaa ggtatacgtt ccctggctat tcttcaccga
     2701 gccacagccg gtgccaggat gggcggttcc acccacgatg ggcgtgacat ttacccctgt
     2761 cgacccttgt gtaggggtga ggttcgtcgt ggtttgccca ccgcgacctc cgccggctat
     2821 agcaaaatag accaggctac cggcagcaat agctagcaat gcggccagta cgcctaataa
     2881 tatgaaagct tttctcctgt tcatgtattt tcactcctct gagattgctg actgatcagc
     2941 ttgttttctc agcaagcctc cttagttttc gcatcaccag gtcataatgt tcaacatcct
     3001 gcgctccaat gtccgagcgc tcaaatggtt ctaccagagc cttgccgtcc aattcatcgg
     3061 gaggtggcac acccataaac gagaggatcg tgggaacaac atcataaaca tgtgcggggg
     3121 tcatggtgac gccctttttc accccagacc catagaggta gagtacaccg tccgggtggt
     3181 ggatgccggt tgagacgctc cgaccaacac cgcgcgtttc ccatagtgat gtgcgaccga
     3241 gctgcgtgta gatagaggta ctctccccgg ataggacaat cacatgccgc tgatccggcg
     3301 ctagctgtcc gcttccaaaa gcatcctcac gatgtatctc gagcgctaat ggctgaccat
     3361 tctccgggtc gcggaacgcg cgtaacgcct cgataaattc gtcgatttcc tcttccgtca
     3421 ccgtgtcgtc gaagtagaca tctgcataac cggccagttc accagaagtg gattgaatcc
     3481 aggcccgcga cttagcccaa tcgatgttag gcaggatggc gacgtgatcc gtttccgcca
     3541 tgtgattgac ctgcttatga cgaaggcgcc gatagagggg cagcgcgata cgctgcaagc
     3601 cgacagcccg tactatcgcc ctggctatcc catagagcct ggcccctctg cgctcactgg
     3661 cattcatttg caaccagccc ttccggtaga ggtattcttg cagataaaac ttgcgcgtca
     3721 cgccctggaa accatgatcg gataccacca tcagcatatc gtcgggcatc aatccatcca
     3781 gcaccttccc cagcgcctcg tcaaccatct cgtagtagcg caccgcggag ggatggaatg
     3841 acatgatctc gtcccagcgc atatgctgga tgcgatcggc tccggtgtag acgatgaaga
     3901 agaaatccca cggcttcttc agcagcaggt tcagcatctc gttccgctcc tgcgtcatct
     3961 tcagggtatc cgtgagtact tcgccgacct ggccattcga cacggcggcg ttgagatcga
     4021 tatgatagcc aggcacctgc tccaacagct cttccttaaa agcgaccgga taggtcacat
     4081 tggccttcat atccggagac atcaagccgc tcagcataat accgttgacc ggctcaggtg
     4141 gataagtcat cggcacattg gcaacgatca cctgtttccc ccaatcgctg agaatattcc
     4201 agatcgccct gccgccctta cggtgagtca tcacatgcgc cgctggacca cttgcggttt
     4261 gctcatactc ccagaagtcg aagacgccgt gtttcgccgg cgccaatccc gtagcgatcg
     4321 aggtccaggc tgggggtgtg acgggcgggg tcgtagattt gagtatgccc tcaactccct
     4381 cagcgcgcag gcgactgaaa ttggggagca ggccgcgctc catcagggga ttcaacacgc
     4441 gaaaggttaa accatcgaca ccgaataaga atactctcat attagtaatc acctttccct
     4501 gtagtgccac ttgtcgcgag cttctttaat ttcttcaaca ccagcccttc gcctggggac
     4561 tccgctgtcg aaggctgctc aaaagcctcg ttgattacct gcccatccaa ttcaggtggc
     4621 agaggcaggc ccatgcgtga caggatcgtc gggacgacat cgtacacctg cgagggcgct
     4681 aaacttacgc cgtgcttgac attcgcgccg tagagataca acacaccatc aggatgatga
     4741 atccccgttg ccgcagcatt cgtttcccag agcgattgac gccccaggtc agtagccagg
     4801 ttacagcttt cgctcgccag caccaccagg tggcgctcac ctggttgatg cgcgccttcg
     4861 ccatacactt cttcgcgata gagttcctgc accagtagct gtcctgtttg cgggtcgcgc
     4921 accgctcgca ggtcgcgcat taaatcgcag atgctttcca cgctcagaga ctcggccagg
     4981 aaaatatcgg catagcctgc cattgacccg gacgacgatg gcacccacgc ctgcgtgcgc
     5041 tgccaatcca ggtcgggcag cttgacggca tggtattcct cttcaacagc caccacccct
     5101 gagcgacgca ggtaacgcat cgtcttacgc gccagtttct tcaatcccag agattgcaca
     5161 aacctcttga tgctgttgat gaagagggcc tggttgcgtc tgccgccatc tctcacgcgc
     5221 agcaaccctc ttttatagag atattcttga atatagaagg tgcgttttgc gccctggaag
     5281 ccgtgatcgg atacgatcat gagcacgtcg tcgctcttca attcggccaa cgccatgccg
     5341 agagcctcat cgagcatttg atagtacgcc actgcttgcg ggtggaactc cagtattttc
     5401 tcccaacgca ggtgctgaat ccggtcagcg ccaacgaaag ctacgaaaca gaaatcccag
     5461 gttttgctca acagcagacg aaacatggcg atgcgttcct gtgtcatttt cagcgtctca
     5521 gcgaacgggt cgcccacctg gccacccgac acggcggggt tgaggtcgat catatactct
     5581 ggcactgcgt cgagcagctc ttgcttaaat tcgccaggat aggtcacgtc cgcgcgcata
     5641 tccggggcca tatagccgct cagcatcatg ccattcaccg gctccggcgg ataagtcatc
     5701 ggcacgttgg cgacgacgac gcgcttaccc cattcgctca ggatattcca gatagctttg
     5761 ctgcccttgc ggtgggtcaa gacatgcgcc ctggggcctt cggctgtttg ctcatactcc
     5821 cagaagtcgt agacgccgtg tttggaaagt gacacacctg ttgaaatgga catccacgag
     5881 ggcggcgtca tgggcggcac cgtagacgcc aggatgccct gcgcaccttc atcacgcact
     5941 cgttggaaat tgggcaataa ccctcgctct atcattggat tcagaatgcg aaaggctaag
     6001 ccgtccaccc caaacaggag tactcgcgtc ataaaaactc tgaccctcca ttcttcacac
     6061 ggaaaaatcc actattgcaa tggcagactc acctgtaagc gctttagttt gcgcgcgacc
     6121 aggtcatccg cgtcgttggt gttattctga acgcgctgaa atacatcctg cgccacatgt
     6181 ccatcaaacg gttcgttggc tgacacgccc acggcctgta aaaccgtcgg cacaagatca
     6241 tagatttgca gcggctcaat gcgcttgccc tggcgcattc ccgcgcccca ggcataaaag
     6301 acgccctctt tttcatggac gcccttaggc tgatcgagcg tctcccataa ctgtttcctt
     6361 cctcgtttca gatggaaggt ggtgcccgat gccggcacga gcacgatatg ctcttccggg
     6421 gggcggaacg ggccaacgcc atacacatcc gttgcgtaga ccgcctccgc cagcaactcg
     6481 ccggtctcag gatgacgctc agcagccagc gcctggcgca gctcctccat gtcctcggca
     6541 gaagctttgg gagacatgaa gatatccgcc atgccgctgc caaaggcggt caatgaaagg
     6601 acacaggcgc gattggcatt ccagtccatg ctctcgaaaa gcggcttata atggctctta
     6661 cttttctcca tcatcggctc gtcgctaaaa tcggtgtaca ctttgcgcgc cttacgcgct
     6721 agattgagca aatgcatctt ttgcgctgct tctctcccga agccaatcag ctttgaccgg
     6781 ctcgattcag cattcccgtc gcgctgcagg agattgcggc gacaaaggta ctcgttgatg
     6841 taaaaccact tccttgctcc gacaaagcca tgatcggaag caacgaagag gacatcctcc
     6901 gcggtcagac ggttcaatac gcgccccagg gcatcatcga gcaagcgaaa gtatttagtg
     6961 ccttcttgac tgagggattc gatggaatcc cagatacgat gctggatgcg atcgggtccg
     7021 acatatgaga ggaaagcaaa atcccagtcg tgttcggaca gcagatgctc ttgcagttga
     7081 atacgcttct cggtcatatc caggacagca tcgacgaagc gacccgccgt tacccgttcc
     7141 gatcccaggt caaccagata gccgggaact gctttgtata attcgtcctt gaaactgtgc
     7201 gggaaggtga acggtgacga ggtactggga gtcatcagcc cactgaccat gatgccgttc
     7261 accgggtccg gcgggtaggt aaggggtaca ttcataacga tcacccgctt accatactcg
     7321 ctgagaatgt cccagacagc cttaccgcct ttgcgatggg tgacggccct ggatttgagc
     7381 gtctctgaat tggtatcaaa ctcccagaag tcgaaaaccc cgtgctttgc cggcttcagg
     7441 ccggtcacca aggacatcca cgccggggga gtaatcgcag gaaccgccga aataaactct
     7501 gcttctaccc cttctcgctg caaacgcgcg aagttgggca ggtctcctcg ttggatgaga
     7561 ggatgcagga tacgaaaact caggccatcc accccaaaga taactgctcg catatatctg
     7621 ttgtccttta catgccgtct cagcgatcgc gctattcgct caacacactg tcgagttgct
     7681 ctgacgaggc caccgctata atttctggtt cgcttgcggt tcgagatggt ctttgaaggt
     7741 ttagctgcgt tcccgcgctt gcatttttcc gcacatgcca ggcaacgcct gccgccatgc
     7801 cgaccgccct accgccaagc tgcgccgcca gaccggtgac acccagcccg accaggaaac
     7861 ccgcccgccg aaaggccgcg ccaatctcca ggaactgacc ggtcacagtg cggaaattga
     7921 agccgaaatt cttgtaggca agataggcgc tattacggcc attagagaac tgaatatatg
     7981 gccgctccat gaaaaatggc tttgcgctgc gtgccgatac atgccgcacg gacatagcag
     8041 gcacaaacat aatccgccag ccagcttttt tcaagcgaat aaacatatcc gtctcttcgc
     8101 gataattcgt cagcgtataa ttagagtcga acccaccaat ctgctcaagc gccgcgcgcc
     8161 tgaacgacat gttgcatccg acaaggtgat cgacctctag cgccgcttcg ctcacaagat
     8221 caaaatccct ggcgatagca atacccgatg gcttcaccag cagactcgga ctgccgtctt
     8281 caagatcaca atacggctcc ggtctgcgaa taattcgacc gccgaccgca cctatcgact
     8341 cgtctttgta ggcgtcaacc atcgcattca gccacaaagg aaaaatcatg gagtcatcat
     8401 cgataaaagc aatcaggtct ccgctcgaaa cggccatgcc ttcattccgc gcatagggca
     8461 tgttattccg ttgcccgcgc aggcgtacac tgatcgtgcc gggatagcgc gcgatcactt
     8521 tctcgctctc atcatttgag gagctatcaa ccacgatgac ttctttatca ggatagggct
     8581 gatcgtaaac ccgttcgagg cagtgttgaa ggaggagagg gcgattgcaa ctgatgatga
     8641 tgatacttac caaaggtctc ttcattgcat attcctcata ttcccaaagg gcatttttga
     8701 tagtgatgag ttctacccaa gatagcccag gtcgcgcaaa cgatcaacga tcattccttc
     8761 ttcctcatcc gagagcaact cgtcgtcgct attgatgcgc tgtatgggtg tctgctcggc
     8821 ataagcgggt gtgaacatgg atgtgatcac attcccatcc acgtccatta tgatgggctg
     8881 ccccaagaga tacatgatcg atggcatgac atcgaccagc gacgcctcgc ccttgaaacc
     8941 cttcgccagg cgcgggccag caccgatcag ccgcgcggca ggagcggaaa caggggatgc
     9001 gcttgcgcgt ggaccgacgc tcgcgccgtc gaaatcaagc gctgccgcct tcggagaagg
     9061 cacatagcca ggtcgataca caaccaccag gtcgggcgac ttgaacaggt aggcccccga
     9121 ataggcttct tcttttctca gcacctgcgc aacaacgggt tctcccgtct gcgggtcgcg
     9181 ccaattgtta cttagttcat tgaccaggac atcacgcacc tgctggtact cgttgccaga
     9241 gcgcacactt ccctctgctt ctcgcccctg taaattaatc cagatttgcc ccgttccaac
     9301 cccataagcc aggctctcag accagataat acccgaccgc tggcgcggct cattgacctc
     9361 gaggatacct ctatccgcta aaaaatcatt gacattcaca ctattgaccg gttgagggta
     9421 ccagacatcc gtaagcacga agaaatgatc gtccgaagag gccagttgac taaagcgctc
     9481 taccagggac tcaagacctg ccgtgtcgag accgcctgcc gcgaaacgga caatgaggca
     9541 atcatacgcc tggtcgaaga gcgcccccag ttcatcgagg gacttcgcct cgagaagagc
     9601 agtggacagc ttgcgcgctt gtaatacatc ctggagcaac cgccccattg cgccttcggg
     9661 aacacctgta tcttcaacga caccgtattt ttcgggtcga acagcatcga atcgtccaaa
     9721 ttttcctgac cccataccgg tcaaggtttg gtaataattc acgccctttt ctgcaattgg
     9781 caacggagta agctgaatat caactccctc attcgccaat gtactcagtc gagggttttc
     9841 tgagagatgt gcaggattga ctccctcgag ccacagaata aacaccgaat tcttcattgc
     9901 cgcaattcct ccgtttcaaa acacgtctta ttagatatcg ccactgagta tgcgcccacc
     9961 tctcaccgtt aacccctcag cattctacag caacgctgcc tgcattccca ttgggatgct
    10021 atcaaggttt ggttaaaaac aggtatccct tgttaagatt tctcaatcga actggaggga
    10081 acgctcaatg ccacccaaaa tctctaccca ccagttcata tagttgagca ttatggggag
    10141 cgaaatacgc tgaaaggcgc tctcttgtcg ccgggtccat tttcgagggt gtgctctccg
    10201 atgattgatt gtatattctg tatccttttt tcagtccctt tggttccagc gaggaaacgc
    10261 ccaggaaccg cagcgtctct gtgtagattg ccgccgggtc ggaatagaaa tcctcagtgc
    10321 gcaagatgag gaattgctca cgtgggaaca ggtccatcca ttttttcagt tgctcggcgt
    10381 acaggcccct ggccaggtag gaggcgcgtt ggtaggcaaa atcatgataa tgctcgtcca
    10441 cctgtgctct cgcttttccc tcttttgtct gctcctcttc aagggccagc gcatcctgga
    10501 aggacatatc ttttatgtga ccccatccca ggttatgacg gtactgtgag tatgccctct
    10561 ccaccggatt acgcagcagc gcgataatct tcacctgcgg caaagtctgc catgcttttt
    10621 gggcgcaatg gggataaaag agatattcgg ggcttccttc gccagtgatg aactctctct
    10681 tcaagacgcg ctcggtgtag tatttttcta acgctgtcgg gaagaacgtt cgataccagg
    10741 atagcccttt gtggaagttg cggtcgaaga aatggacctc tttcctcctg gccgatttga
    10801 tgctgggatg ctcgatcagg tagttgtaaa gagaggttgt tcctcccttt tgagcgccga
    10861 taatgataaa atccggcaaa acacgccagg ggctgctgga acttcggaaa tatgtgtcta
    10921 tttttctagc ctcatcggtc aatcgagaca tggtttcgac tccttcatac tgctatttga
    10981 ttcctccagc gaggggcata acacagatag atttattctg catgggacac aacgacctta
    11041 ttgagtgggg agagcgtatc cagttgcggc ggagagagta gctgttcctc ctgaatcgcc
    11101 tctttttccc agtttggtat ggtaatcacc aggcccagga aaagccagtg atacgatgcc
    11161 acgattaatc ccgccgtccc accacctgcg gcggcagaag ggatcaagat accggtaaaa
    11221 aagccgccga agatgaagca gaggtagaac ccgacgaagg agatcgtcag tgacttgctc
    11281 aagcgggtct ttgccccttt gaaggattta aaagcgatca cgccgaacga tgccagcatc
    11341 cagagcaggg cgattaaccc ctgaacaccc atttctgcta atacttcgag atattgatta
    11401 tgcgcgagtc ccgctttatc gacaccatat atccaatcat agaactggta attgcccgcg
    11461 ccgataccaa agtaagggct ttttagccag atcgtaatgg catcggccca gattttcaag
    11521 cggtcagagc cgtagacttt cgaggaattg aagaaatcgc ttactcctgg cacgaataga
    11581 agcgcgatag gcaggagcaa gaaaagcaac gccgcccaat tcttggtacg gaacaccagc
    11641 atgaccagca tcgacataaa gagttccagc catgcctctc gcccgaaggc caggatgacg
    11701 ccgatagcaa aaagcagggt gagtagcccc cagccgagtc gacgcctgcc ctccgaatag
    11761 agcgcctggc cgaacgccag gtcggtaaac agaacgagcg tcgtgccaag cccgctggaa
    11821 tcaaggccaa acacctcgac gcgcctgacc cccaggatga ccttcttcga gtaaaagccc
    11881 agcggggccg caaaaattgt gccaagcgta tacagcaccc cggcaacaat aaaagcccgg
    11941 aaagccaatt tataatctcg cacagtgcgc accattttac gcgcaatgat caaaagtaga
    12001 ggcaggccaa gcagtaaagc catttccgac aggttcacga ttgtgatcga aacgtttgaa
    12061 tggggatagg tgtagggtac attcgggtcc gggtaaatgc gcgaataaat aatggagatc
    12121 agcccaacga tatttattag aagcaacgga atcagcagtc cccttgacag gagtttcgat
    12181 ccgcttttgc gttcaggcag gacaaattgg gccagccaga tgattgccag cagcgccaaa
    12241 agcatgttgc cgatgtaaag gcgagatagt atacccgatt gcgaaagagt tatagaaagg
    12301 ctggggcctg ccacaatgat atagagtggg agcgcaaaac gagatcgcaa aaaaaggaag
    12361 acgactgcat tgatcgcaac cgcgcccaac agcccaagcg ccaggttgat cgtcataaga
    12421 tatgccagag ccaggttcaa aacaagataa atgatcaatg tcatgatcac tgcgatggaa
    12481 gtgagcctga agttaagtga tgataaaaaa gaaagccttc tcatttagtc ctcattaata
    12541 gtcaaactgt acctgccatc aagcattttt gcaggccgtt gaatagctgg tttacacatg
    12601 gtgacacctg gaatcgccct attcagcgca atccaaagtg gtcttatact accatactgc
    12661 gagtacgatt ctgcattcct tctgtagcct tgaccggcac ttttagtact aatttaccat
    12721 ctatgacaac agtatcggat cacgcacgcg agttcaatag tatccgtgac acggacttaa
    12781 tgctcctcaa aatttacaaa gacctggccg atagtatgtt atatcttcga tacctgaaaa
    12841 ggtggttata attcctcaac gttgtccagt tgatcatcac cggatcataa ccataatttc
    12901 agatagataa gattggatga agggtatagt ctagtcgtct cacatgttta taaggcttgt
    12961 tgttgagagt ctggggtcaa tggaatgacc ggttagtata aaggcagtgt ttttctatgg
    13021 tccaacaatc cgagatagcg cctcgcagac cttcggcgat tttattaccg ccaaaatccc
    13081 cggtgaagta ttttcaccgg ccacggcttc tgcctgtgaa cgaggcgttt gaaaaagaga
    13141 cactgcatat gtcaactttt cacgtggaaa ttccgcctga gatgttgacg acctcacccc
    13201 tctttgcaac agtggtagaa cgaacagaaa ctcagccgct ccaggcgttt cagcagcagg
    13261 cgggagtcga atccaggctt cgcaatcggg aattcgtacc cgatacgccg gatcacgagg
    13321 tctactcagt atctcagttc ctggaacacg cgaccattcc gctgatcgcc tccgaagcca
    13381 caacaaacca tgcggagccg gcagcgctcg tgacgaaatc ggagatcagc ggcactgcta
    13441 ataatgcggc gatcgttggg gttggcaata tcgtcggctt ccttttcaag tatggcaaca
    13501 acctgttgat tcaacacgca ttgggggtgg cgcttttcgg gttgtattcc gtcagcctgt
    13561 cgctggtcac attgctagga tcgatctttg acctcggcct ggataacacc gtgattcgct
    13621 atatctcggt ctatcgaggg aaaaaacaga agagtatgat gcgccatctt ttaacgttct
    13681 gcaccgggat ggcggcgatt accggaacgc tcggcgcagt gttggtactt tttttcgcct
    13741 cctccctggc caatttcgtc cataaaccag ataccattcc gctcctgcaa attatggcac
    13801 cggtcgttcc actcttatgc atgcaaaccg tctggacgag tggcttacag gggttaaagg
    13861 aattcaaaag gcgcgtactt atccaacgct ttgtcgtccc cttgacgttg tttgctctca
    13921 cggccatcgc gctctttttc tttcgcaatc taacgggtgt agctattcta gccttttgca
    13981 gcacgttgat cagcaccatc atcaatctgt atttcctctt cgccgcggtt aaccgcatta
    14041 agggatcaga ggatggacgt tacgaaacgc gctcgtggct gagctttgcg ctcccgaatt
    14101 tcttaactac catcgtcaat actgcccttg actcaatcga tacactcttg ctggcatttt
    14161 tcgtcgccgc taacgccatc ggactatacg cggctgcgat caagatcagc aacttcatcc
    14221 tgataccgtt ggccacgatg aatatcgttt tcacgccgac gatcgcagaa ctctt
//
LOCUS       DASZAK010000762         4832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_54042, whole genome shotgun sequence.
ACCESSION   DASZAK010000762 DASZAK010000000
VERSION     DASZAK010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54042"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..637
                     /locus_tag="VGT44_13420"
     CDS             116..637
                     /locus_tag="VGT44_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581847.1"
                     /translation="MPAMHNSIQVNNVLGLIVVLLLWPGLVVCSRLLISSLRNDPLIC
                     WAIGPLGLTTLYLSELSSPFIVLSALFPAIVSGVVLYFGLFSTLPSPLELPHRLLVEI
                     PVIAVGVMLTSALDLLTALRDLRHPIWGEARVLRTIQFLRASWASIHFTAFGITFLRD
                     HFDSNPTDLLKAF"
     gene            complement(642..2435)
                     /locus_tag="VGT44_13425"
     CDS             complement(642..2435)
                     /locus_tag="VGT44_13425"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2581848.1"
                     /translation="MQPQPKVFTAIDPSLIIQYEDRLKTFHQQMAFLNTIGKRFVAAN
                     DRFQVHRALLATLRELYHLTACSILLEGDPVELTFTLSIIPCYPLGEPFIHGMIERIA
                     RAAAVMGFTGITEEELAILAYFDAPDDISQLVEAAGLSAEYAESSPPEVNSIGVCLNI
                     PLTVENRIIGVLSLFDEHEDAFDAELLQLTTMIADYAAVALENVSLREREMTLLRDAV
                     LERQRLELVIGSMAEGLLLTNEKGAITSLNNSAQRLLAQTELCLPQDSLTPLRLLAKQ
                     YEAKWIADLAEIIDQALTGKTIMNQEIMLGAVEDAVPLTLSISAAPLYDSNAARTILL
                     GVVAVIGDITSHKQIDKLKDEFVSIVSHELRTPLTAIKGYTQHLIRRIERRLREQRQL
                     QQAAQSGQGVADLPESYDLRSLNIVQSQTDHLERLVNDIIDLSRVQSGDLQLRLTSFY
                     LADVLTESAHQAQISAEQHMISLEIAAHDSRVVADKVRVSQVIGNILDNAIKFSPQGR
                     QVVVKLQEREHDYLVSVSDQGIGVNPEYFDHIFERFYRVRNTASRQYSGIGMGLFIAK
                     MIVEAHGGSIWLTSSQGGGSTFYFTLPRILN"
     gene            complement(2457..2840)
                     /locus_tag="VGT44_13430"
     CDS             complement(2457..2840)
                     /locus_tag="VGT44_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581849.1"
                     /translation="MQSSFSSGHSSSSYLEKVTQAYLLAIQTIDDRVTPFLGKVTTRV
                     LVQGAARRVASSYPFLQFLEKMPYTDVSPSVVREQLGGVPQQLLSTGLDALLQECFAG
                     LRELTGDLIVPPLHEEITRQLEQLP"
     gene            complement(3022..>4832)
                     /locus_tag="VGT44_13435"
     CDS             complement(3022..>4832)
                     /locus_tag="VGT44_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2581850.1"
                     /translation="LQRIREMVELPLKYPAVFDRLGVEPPKGVLLYGPPGTGKTLIAR
                     AVASETNAAFFVINGPEIMNKFYGESESRLRSVFQEAQKRAPSIIFIDELDALAPKRS
                     DTGGEVERRIVGQLLALMDGLASRGQVVLIGATNQPNALDPAMRRPGRFDREISLRVP
                     DVRGRTEILQIHSHDAALASDVDFARLAQLTPGFVGADLEALCREAAMIALRRVLPHI
                     DYEKGYIPYDTLVNLYITMADFQAALREVEPSTTREVYVEVSETSWDDIGGLDKVKQL
                     LTEGVEWPLRYPEIYAHARVEAPRGVLLSGPPGSGKTLIARALAHQCEASFISIKGPE
                     LLSKWVGESEKGIREVFRHAKQAAPCIVFFDEIDALAPHRGGDIDGHSGDRVIAQMLT
                     EMDGVEGRAGVIVVAATNRSELIDPALLRPGRFDLVVELGYPTEADRRSIFAIHTKGR
                     PIAPEITVEDLARMTEDRSGADIEAICRRAGLLALRDWIAPRLVPGRVQITEESTVVD
                     VNAENVQSESPADSAASPASLNQRTPLLILPKHFAAAIEEQRERYDTQREAEEARARK
                     DAGRQRLIDMAANMNAGSKPPLRGIRLWLARLFRLV"
BASE COUNT          973 a         1437 c         1392 g         1030 t
ORIGIN      
        1 tcggacagtg ggcgaccctt gcggtcgccc tggtatcgga cagtgggcga cccttgcggt
       61 cgccctggta tcggggtgac acaagggtcg ccctggaaca aaacaggaga ataccatgcc
      121 ggccatgcac aatagcattc aagtgaacaa tgtactgggc ttgattgtgg tcctcttgct
      181 ctggcctggc ctggttgttt gcagccggtt gctgatctcc tccctgcgca acgacccgct
      241 gatctgctgg gcaatcgggc cgctcggcct caccacgctc tacctcagcg aactttcgtc
      301 gccctttatt gtattgagcg cgctgttccc ggccatcgtc tccggcgtcg ttctctattt
      361 cggcctgttc agcacactgc ccagcccgct cgaactccct catcgcctgc tggtggaaat
      421 tccggtgatc gccgtaggag tcatgctaac cagcgcctta gacctgttga ccgcgctgcg
      481 cgatctgcgt catcccatct ggggcgaggc gcgcgtgctg cgcaccatcc agttcttacg
      541 cgccagttgg gcctctatcc acttcacagc cttcggtatc accttcttgc gcgatcattt
      601 cgattcgaat cccaccgatt tgttgaaggc gttttaaagg gttaattgag gatgcgtggg
      661 agggtgaaat agaaggtgct gccgccaccc tggctactgg tgagccagat acttccgcca
      721 tgagcttcca caatcatttt tgcgatgaac aatcccatgc cgatgcccga atactggcgg
      781 ctggcggtgt tgcgcacgcg gtagaaacgc tcgaagatgt gatcgaaata ttccgggttc
      841 acaccgatgc cctggtcgct gacactcacc aggtaatcat gctcgcgctc ttgcagcttg
      901 accaccacct gcctgccctg cggtgagaac ttgatggcgt tatcgaggat gttgccgatt
      961 acctggctca cacgcacttt atcggccact acgcggctgt cgtgagcggc gatctccagg
     1021 gaaatcatat gctgttccgc gctgatctgt gcctggtgcg cgctttcggt caggacatcg
     1081 gcgaggtaga agctggtgag gcgcagttgc agatcacccg attggacgcg cgacaggtcg
     1141 atgatatcat tcaccagccg ctcgagatga tcggtctggc tctgcacaat gttcaagctg
     1201 cgcaaatcat agctctccgg caaatcagcc acgccttgcc cgctctgtgc tgcttgctgc
     1261 aactgacgtt gttcgcgcag gcgtcgttca atgcgcctga tgagatgctg cgtgtaaccc
     1321 ttgatggccg tcagcggcgt gcgtagttcg tgcgaaacaa tcgacacgaa ctcgtccttc
     1381 agcttgtcga tttgtttgtg cgaggtgata tcgccgatca ccgctacgac gcccagcagg
     1441 atcgtgcgcg ccgcgttcga atcgtagagc ggagcggcgc tgatgctgag ggtcagcggc
     1501 accgcgtcct cgaccgctcc cagcatgatt tcctggttca taatggtctt gccggtcaaa
     1561 gcctggtcaa tgatctccgc aagatcggcg atccacttcg cttcgtactg cttcgccagc
     1621 aagcgcagcg gcgtcaggct gtcctggggc aggcagagtt ctgtctgggc cagcagcctc
     1681 tgcgcggagt tattcaagga cgtaatcgcg cccttctcat tggtgagaag caagccctcg
     1741 gccatactgc cgatgaccag ttccaggcgc tggcgttcca gcacggcatc gcgcagcagg
     1801 gtcatttcgc gctcgcgcag gctgacattt tccagcgcaa ccgccgcgta gtcggcaatc
     1861 atggtggtca attgcaggag ttcagcatcg aaggcatctt catgttcatc aaagaggctg
     1921 agaacaccga tgatacgatt ctcaactgtc agaggaatat tcaggcaaac gccgatgcta
     1981 ttcacctcgg gtggcgacga ttcggcatac tcagcggaaa gaccagccgc ttcgaccagt
     2041 tgcgaaatgt catcaggcgc atcgaaatag gccaggatcg ccagttcctc ctcggtgatg
     2101 ccggtaaagc ccatcaccgc tgccgcgcgg gcgatgcgtt cgatcatgcc atggataaat
     2161 ggctcgccga gaggatagca tgggatgatg gagagggtaa aggtcaattc gaccgggtcg
     2221 ccctcgagca ggatcgagca ggcggtcagg tggtagagtt cgcgaagagt cgccagcaga
     2281 gcgcgatgca cttgaaaccg gtcattcgct gccacgaaac gcttcccgat agtattgagg
     2341 aacgccatct gctgatgaaa cgtcttcaag cgatcctcgt attgaatgat gagggaggga
     2401 tcaattgcag tgaaaacctt cggctgtggt tgcacgctac cctccataca agctctttat
     2461 ggaagctgtt ccagttgccg cgtgatttct tcatgcagcg gcggcactat gagatcaccg
     2521 gttaattctc gcaatccggc aaagcattcc tgcaataacg catccagccc ggttgagagt
     2581 agctgctgcg ggacgccacc cagttgctcg cgcacaaccg atggactgac atcggtgtac
     2641 ggcattttct caagaaattg caggaatgga tacgatgaag caacacgcct ggcagcgccc
     2701 tgcaccagca cgcgcgtagt gactttcccc agaaaaggcg tgacccggtc atcaatggtt
     2761 tggatggcca gcagatacgc ctgggttacc ttttcaaggt atgaggaaga ggagtgacca
     2821 gaactaaatg atgattgcat tgctgtctct cccttcgctg tctcccacaa tcttctctcc
     2881 acttgtatta taacacagtc caataactta ttgtaagtgg gcaagtcaag acaatattgc
     2941 acagattaca acaaacacgc ttataacgcg ctctaattgt aatgacattg tttctgtgta
     3001 atatatgaca atgtaaagaa atcacaccag tctgaacaaa cgggctagcc ataagcggat
     3061 accccgcaga ggtggcttgc tgcctgcgtt catattcgcc gccatatcaa ttaaacgctg
     3121 gcgacctgca tctttgcgcg cgcgcgcctc ttccgcctca cgctgcgtgt catagcgctc
     3181 gcgctgctcc tcaatcgcgg cggcaaagtg cttcggaaga ataagcaatg gggtgcgctg
     3241 gttgagtgac gccggggatg ccgcgctatc agcgggcgat tcactttgaa cattttcggc
     3301 attcacatcc accactgttg attcttcggt gatctgcaca cggccaggga cgagccgggg
     3361 cgcaatccaa tcgcgcagcg ccagcaatcc ggcgcggcgg cagatggctt cgatatccgc
     3421 gccgctgcga tcctcggtca tccgcgccag gtcctccaca gtgatttccg gggcaatcgg
     3481 acgccctttg gtgtggatgg cgaagatcga gcgccggtcg gcctcggtgg gatagccaag
     3541 ctcgacgacc aggtcgaagc ggcctgggcg cagcaaagcg ggatcgatca actcagagcg
     3601 gttcgtggca gcaacgacaa taacgcctgc gcgcccctcg acgccatcca tctcggtcag
     3661 catctgcgcg atgacgcggt cgccagagtg gccatcgatg tcaccgccgc gatggggggc
     3721 cagcgcgtcg atttcatcga agaagacaat gcacggcgcg gcctgtttcg cgtggcggaa
     3781 gacttcgcga atgcccttct ccgactcacc gacccacttg gagagcagct ccggcccttt
     3841 gatcgaaatg aaactggcct cgcactggtg cgccagcgcg cgcgcaatca gcgtcttgcc
     3901 cgatccggga gggccggaga gcagcacccc gcgcggcgct tcaacccgcg cgtgggcgta
     3961 gatttccggg tagcgcagcg gccactcgac gccttcggtc agcaattgct tgaccttgtc
     4021 cagtcctccg atatcgtccc aggacgtttc gctgacctcg acatagacct cgcgcgtggt
     4081 ggacggctcg acttcgcgca gggctgcctg gaaatcggcc atcgtgatat acaggttgac
     4141 gagggtgtcg taggggatgt agcctttctc gtaatcgata tggggcagca cgcgccgcag
     4201 cgcgatcatg gcggcctcgc ggcacaacgc ctccaggtca gcgccgacaa agccgggcgt
     4261 gagctgcgcc agtcgcgcga aatcgacatc gctcgctagc gccgcgtcgt gactgtgtat
     4321 ctgcaaaatt tccgtgcgcc cgcgcacatc cggcacacgc agcgagattt cgcggtcgaa
     4381 gcgccccgga cggcgcatgg cggggtcaag cgcgttgggc tggttggtcg ctccgatcag
     4441 caccacctgc ccgcgcgagg ccaggccatc catcagcgcc agcagctgcc caacaatacg
     4501 ccgttcaacc tcgccgcccg tatcggagcg tttaggggcc agcgcgtcaa gttcatcgat
     4561 gaaaatgatg ctgggcgcgc gcttctgcgc ctcctggaag accgagcgca ggcgcgactc
     4621 gctttcgccg tagaacttat tcatgatttc cgggccattg atgacgaaga aggccgcgtt
     4681 tgtctcggat gcgacggcgc gcgcaatcag cgttttgccc gttcccggcg ggccatacag
     4741 cagcacaccc ttgggcggtt cgacgcccag gcgatcaaac accgccggat acttcaaggg
     4801 cagttcaacc atctcacgaa tgcgttgcag tt
//
LOCUS       DASZAK010000763         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_588278, whole genome shotgun sequence.
ACCESSION   DASZAK010000763 DASZAK010000000
VERSION     DASZAK010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588278"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1019
                     /locus_tag="VGT44_13440"
     CDS             141..1019
                     /locus_tag="VGT44_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2581851.1"
                     /translation="MVAQSSQAPTAQERRSAHVIHYDISYIEQGAEHGSDGAIVLLHD
                     ILGGAFTWDGVLARLGGAGRAVYAIDLLGYGQSDRPWPADTSVWGHADCLTFLFKQLN
                     LTNVVLVGHGIGGGVAQILATRLCPERIAALVLVNSICYEYTFAPNWPLSDMKKYQDP
                     EIANELELKDLIADLEATAPNGAHKTDDLKQDIDKYAQPWNDHIGEEVFLQQIRLLIP
                     SYSNSVSSDLRHIGKPCLILWGEKDAQIPLKFAQRLHRDIPESRLVIIPDAGHLILFD
                     VPAQVASALLDFVGRL"
     gene            complement(1031..1240)
                     /locus_tag="VGT44_13445"
     CDS             complement(1031..1240)
                     /locus_tag="VGT44_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581852.1"
                     /translation="MTLRELERAENAMQQQWHDLVMAEQQGEALDRLEQLYDNYILLA
                     EEYNRCRDAYEASRQQRQQRQRKAA"
     gene            complement(1285..2010)
                     /locus_tag="VGT44_13450"
     CDS             complement(1285..2010)
                     /locus_tag="VGT44_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581853.1"
                     /translation="MNEQQLEHSGNGRGEARAFNPNEHLMQIKNRGGSADYLPVQWRL
                     VWFNEQCPEGKITIVEKIIDPEREVEKEVMQWNNDTRRSERVTKRARGWAYFHVRVED
                     GKGKVGEAVKSESAVDFDDYIEKADTGATGRALAKIGYGTQFAPELDEQHRIVDAPVD
                     RGPAPVETNGNGRKPIAALNRQNDNGQATDQQLASLRKLCQHLGKPEPENLDTLTHQA
                     ARELILQLSHEYNEQRQSSRKAS"
BASE COUNT          439 a          680 c          601 g          558 t
ORIGIN      
        1 gtgcctgtcc gcgttggagg tcacgatgcg cttgccacct ggacgcgggc aggcacaagg
       61 cccccgcccc tccgctctgc cacccactgt ccctacagaa atagattcga aatagtggtt
      121 atcatttgtt gagaggtaat atggtagcac agtcttcgca agcgccgacc gcgcaggaac
      181 gtcggtcggc gcatgtcatt cactacgata tcagctacat cgaacagggc gcagagcacg
      241 gtagcgatgg cgctatcgtg ctattgcacg acatcctggg cggcgccttc acctgggatg
      301 gcgtactggc gcgcctggga ggcgcaggcc gcgcggtcta cgccatcgac ctgctgggct
      361 atgggcaatc cgaccggccc tggcccgccg atacatccgt ctggggccac gccgactgcc
      421 tcaccttcct gttcaagcag ttaaatctga cgaacgtggt gctggtgggt catggcattg
      481 gtggtggcgt ggcgcaaatt ctcgctacgc gcctgtgtcc agaacgcatc gctgcgctgg
      541 tcctggtcaa ctcgatctgc tacgagtata ccttcgcgcc gaactggccc ctgtcggata
      601 tgaaaaagta tcaagacccg gagatagcga atgaacttga actcaaggac ctgattgccg
      661 acctggaagc gacagcaccc aacggggcac acaagaccga tgatctcaag caagacatcg
      721 ataagtacgc gcaaccctgg aatgaccata ttggggaaga ggtattcttg cagcagatac
      781 gcttgctgat tccatcttat agcaactcgg tctcatccga cctgcgccac attggcaaac
      841 catgtttgat tctctggggc gaaaaggatg cgcaaatacc tctgaaattc gcgcagcgcc
      901 tgcaccgcga tattcccgaa tcgcgcctgg taatcatccc cgacgccggt cacctgatcc
      961 tcttcgatgt ccccgctcag gtggcaagcg cgctgctcga tttcgtaggg aggttataga
     1021 ggtctctttt tcaggcggct ttgcgctgcc tctgctgtcg ttgctgccgc gacgcctcgt
     1081 aagcgtcgcg gcagcgatta tattcttccg ccagcaaaat gtaattgtca taaagctgct
     1141 cgagccggtc gagcgcttcg ccctgctgtt ctgccatgac aaggtcatgc cactgctgct
     1201 gcatggcgtt ctcagcccgt tccagttctc gtaatgtcat gaccggttct ccctgtccag
     1261 ttgtagctat gccgcccgtg ttgcctacga agctttcctg ctgctctgtc gctgctcgtt
     1321 gtactcgtgg ctgagctgca agatcagctc gcgcgccgcc tggtgggtca gcgtatcgag
     1381 attttccggc tccggtttgc cgagatgctg gcaaagcttc cgcaggctgg cgagttgctg
     1441 atcggtagcc tgaccattgt cgttctgccg gttgagcgcc gcaattggct tgcgcccgtt
     1501 cccattcgtc tccaccggcg ctgggcctcg gtcaacgggc gcatcgacaa tgcgatgctg
     1561 ctcatccagc tcaggcgcga actgtgtgcc gtagccgatc ttcgcgagcg ctctgcccgt
     1621 cgcgccggta tcggccttct cgatataatc atcaaagtca acggcagatt cgctcttgac
     1681 ggcctcacca accttgccct ttccatcctc gacccgcacg tggaagtagg cccagccgcg
     1741 cgcccgcttc gtgacgcgct cgctccgccg tgtgtcattg ttccactgca tgacctcttt
     1801 ctccacctcg cgttccgggt cgataatctt ctccacaatg gtaatcttgc cctcgggaca
     1861 ctgctcatta aaccagacca gtcgccactg caccggcagg taatccgcag acccaccacg
     1921 atttttaatc tgcatcaggt gttcgttggg gttaaatgcc cgcgcttcac cacgcccatt
     1981 tccgctatgt tcaagttgct gctcgttcat ttactgctcc ttctgacctg ttttagaaat
     2041 tatgttctaa aaccattata caagagacgc ccaaacaagt caagtttggc tacgaatcta
     2101 ttcttctcgc gggggtgaaa agcggttaga tggccttcct gctaattgtt ctgatagatt
     2161 tgataatgga gaactttcta ttttgcattt catgaggttt tgtgctattc tcttggcgga
     2221 aaaactccca cgaaaggact tggctatggt tgatcgtgtc ggtcaacagc ttggtaat
//
LOCUS       DASZAK010000764         5504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_624703, whole genome shotgun sequence.
ACCESSION   DASZAK010000764 DASZAK010000000
VERSION     DASZAK010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624703"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="VGT44_13455"
     CDS             <1..1346
                     /locus_tag="VGT44_13455"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2581854.1"
                     /translation="DARPITRWPDRDDAFEDVVREVSRAIKALPIHKSKEEWLAEGQA
                     HYKANRFDEAIVAFSNAIKRDASYALAYQHRGEASYRLTNFSEALSDFEQATNLDPML
                     IRAYTWKGDALTGMNESRKAIMAYAEALTVAEKTPPDQQDAGFSFYRGDALLGLKIYA
                     EAMVDYEQAIRMDKHYSIAFKGQGAVLYYLKRYDEAVHMNEQVHQLDPDDAIAYSNKG
                     YALKELKRYDEALAAFDRAIQLDPHLTFAYNGKGLVLSDLKRYDEALAAFDLSLRLDP
                     NYSSSYTCKSTIFNNQQRHDEALAACKRAIQLDPNDSSAHNNMGYALHAMQRYNEAVA
                     AFDLAIRLSPDATNALVGKGDTLFMLARYEEAAAAFEQAIRPDSLDAYAHNGRGSALR
                     NLKRYKEALTEFEQAIRLDPAFIDAYNGKGNVLKELGRNDEAQQAFAKARQLGLQ"
     gene            1523..2887
                     /locus_tag="VGT44_13460"
     CDS             1523..2887
                     /locus_tag="VGT44_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581855.1"
                     /translation="MSKIKMRCTTCGKWFQSANAKEVTCPDCVQKARKEKLAAKNAPA
                     ATEKSVGTTIPTRVAPPPPPKPKPETSGTSHWFDSVSDVKIGEPDKPPQRPRIPGSPA
                     PHEPRESREPYEQRSPGSYRDRDERDSGGYRPAGPGGYRDRDERGPVGYRPGGYRDRD
                     ERGPGSYRPAGPGGYRDRDERGPSSYRPGGSGSPSTLDAPGYRPRQPMEGGPGRGPRP
                     MGPRPDGKRGGKPPRAKTPTPPKPKREKIPPPTPFVATPEQIAQIETRYAELAVPQEF
                     DGIRTQIAKELGIPKKAVKKAVQEFRTRNNKPSWWEMQSYKGDSEELAKIKAAYEPYL
                     PVPVVGIHKKIADELGLKAGDVYQAVKAIRLEMNLPQFNDPELHAEELAARQKQKEAE
                     KQAATEVGQEAGAEATPQAEQIRSVDTTSEGEKPTETHEVAAKNEETPQAAQDEGASE
                     PSDS"
     gene            2954..3298
                     /locus_tag="VGT44_13465"
     CDS             2954..3298
                     /locus_tag="VGT44_13465"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEV2581856.1"
                     /translation="MEDCLFCKIVAGQIPAKIVYQDEEVVSFVDINPQAPQHVLVIPR
                     RHIASMPDITGEDGPLLASIFMAATKIARELGVAESGFRVVTNVGPDAGQSVFHLHFH
                     LLGGRRLAWPPG"
     gene            3322..4503
                     /locus_tag="VGT44_13470"
     CDS             3322..4503
                     /locus_tag="VGT44_13470"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV2581857.1"
                     /translation="MRIAIDYTAATRQAAGISNYVRSLVDAMLAQDTTNQYTLLTSGR
                     PPQDRPFPDAKNVRGRSLVIPDRYLNILWYRWRLPLSATFFTGQVDLYHGPDFVLPPL
                     NGKLRKIVTVHDLAFLEHPEYAVAALAAHLKKVVPEAVAAADAVVAVSGETRRALVEQ
                     FATPPQKITLVPNGIRSSLKRVTNPILLEAARHKYGLKSPLVLGVGTLEPRKNHLGLI
                     KAFHKSASSANKQARPAMLAIAGGEGWMYEETRQAVADLKLEKKVRFLGRVSDLDLML
                     LYSLADVFVFPSFYEGFGVPPIEAMACGAPVITSNVSSLPEVVGDAALLVDPHNNGAI
                     AQAILRVLGNPQQAEELRQKGYERAQRFTWAASARKMLSIYARLYAGKTTFDEEVTET
                     L"
     gene            4500..>5504
                     /locus_tag="VGT44_13475"
     CDS             4500..>5504
                     /locus_tag="VGT44_13475"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV2581858.1"
                     /translation="MKIGLNAMMFRFPATGSGQYLTHLINTLAEIDQQNEYLLLGPDP
                     FPEQQGIDPRLAYQQAFVPAPIRQNLRLEKLAWEQWTAPSQARKAGVDLLHVPYFAPP
                     YFSRLPVVTTIHDVIPIRLAAYRYSGFMRAYLRMVGRAAHKSTLIITVSQHAKQDIMD
                     ALNLPAERIRVIYQAASKEYQPITDAAELARVKARYGVGSRFIFYLGGLDARKNVPAL
                     VRAFAHVYQRLGDPDLQLLIAGNPDKQRGPLFPDPRPLAADLGMSGQIVYRFIEEEDK
                     PAMYSAANVFIFPSLYEGFGLTPLEAMCCGAPVVCSNRTSLPEVVGDAALLFDPDNTG
                     Q"
BASE COUNT         1227 a         1771 c         1552 g          954 t
ORIGIN      
        1 ccgatgccag gccgatcacg cgctggcctg accgcgacga cgccttcgag gatgtcgtca
       61 gggaggtcag ccgtgccatc aaagcgctgc ccatccacaa gtctaaagag gagtggctgg
      121 ctgagggcca ggcgcactac aaagccaacc gcttcgatga ggccatcgtc gccttcagca
      181 atgccatcaa gcgcgacgcc agctacgccc tggcctacca acaccgggga gaagccagct
      241 atcgcctcac caacttttcc gaggcgctgt ccgactttga acaagccacg aatctcgatc
      301 ctatgctcat tcgcgcctat acctggaaag gcgatgcgct gactggtatg aacgagagcc
      361 ggaaagcgat tatggcgtat gccgaggctc tcaccgttgc cgaaaaaacg cccccggatc
      421 agcaggatgc cggcttttcc ttctataggg gagacgcatt gcttggtctc aagatctatg
      481 ccgaagcaat ggtcgactac gagcaagcga ttcgcatgga caagcattac tccatcgcgt
      541 tcaaaggcca aggggcggtg ctgtactacc tcaaacgcta cgacgaggcc gtccatatga
      601 acgagcaggt gcatcaactc gacccggatg acgccattgc ctacagcaac aaaggctatg
      661 ccctcaagga acttaagcgc tacgatgaag ctctcgcggc atttgaccgg gcgatccagc
      721 ttgacccaca cctgaccttt gcgtacaatg ggaaaggtct cgtcttgagc gatctcaaac
      781 gctacgacga ggccctggcc gcctttgacc tgtcacttcg cctcgacccc aactattcct
      841 cttcctacac ctgtaaaagc acgatcttca ataaccagca acgccacgat gaagccctgg
      901 ccgcctgtaa acgagctatc cagctcgacc ccaacgattc tagcgcccac aacaacatgg
      961 gctacgcgct acacgccatg cagcgttaca acgaggccgt cgcagccttt gacctggcca
     1021 ttcgcctttc tcccgatgcg accaacgccc tggtcggcaa aggcgacaca ctcttcatgc
     1081 tcgcccgtta cgaggaagca gcagcagcct ttgaacaggc catccgaccc gactctcttg
     1141 atgcttacgc ccacaacggc aggggttccg cgctcaggaa tctcaagcgc tataaagagg
     1201 ctctcacgga atttgagcag gctattcgcc ttgaccccgc ctttatcgat gcctacaacg
     1261 gcaaaggcaa cgtactgaaa gagcttggaa gaaacgacga ggcgcaacag gcgttcgcca
     1321 aggcccgcca attgggattg cagtagcagg gtgttgctgg ccatccccct ggctacacgc
     1381 tgccaaaaac attgacgctt tcaagaaaca tgctatacta agagcgcatt ccgacacctt
     1441 gacaggattg tcaattcgtt gtaagcaggg tgatgagaac gtcacccgct agtacaccaa
     1501 gaacattcag gggagtttgc gcatgagtaa gatcaagatg cgctgcacta cctgcggcaa
     1561 gtggtttcag tcggctaatg cgaaagaagt aacttgcccc gattgcgtgc agaaggccag
     1621 gaaagagaag ttggcggcga aaaatgctcc ggctgccaca gaaaaatccg tcggtaccac
     1681 cattccaacg cgcgtcgcgc ctccaccgcc accgaagccc aaaccggaga cgagtgggac
     1741 aagtcactgg ttcgacagcg tcagcgatgt gaagatcggg gaaccggaca agcccccaca
     1801 gcggcccagg atacctggct cccctgctcc tcacgaacct cgcgaatcga gggaacccta
     1861 cgagcagcgc agtcccggca gctatcgcga ccgcgacgaa cgtgactccg gtggctatcg
     1921 ccccgctggc cctggcggct accgcgaccg cgacgagcgc ggccccgtcg gttatcgccc
     1981 cggcggctat cgcgaccgcg acgagcgcgg ccccggcagc tatcgccccg ctggccctgg
     2041 cggctatcgc gaccgcgacg agcgcggccc cagcagctat cgccccggcg gttcgggcag
     2101 ccccagtacg ctcgatgcgc ctggatatcg cccgcgccag ccgatggagg gcggtccggg
     2161 acgcggacca cgtcccatgg gacctcgccc ggatgggaag cgcggcggca agccgccgag
     2221 agccaaaacg cccacgccac caaagccaaa gcgcgagaag atcccgcctc ctacaccatt
     2281 tgtcgccact cccgaacaga tcgcgcagat cgagacgcgc tacgccgaac tggccgtgcc
     2341 gcaggagttc gacggcatcc gtacgcagat cgccaaagag ctgggcatcc cgaagaaggc
     2401 cgtcaagaag gccgtgcagg agtttcgcac gcgcaacaat aagccgagct ggtgggaaat
     2461 gcagagctac aaaggcgata gcgaggaact ggcgaagatc aaggccgcct acgaacccta
     2521 tctgcccgta ccagtggtcg gcatccataa aaagatcgcc gatgaactgg ggttgaaagc
     2581 cggtgatgtc taccaggccg tcaaagccat ccgcctggaa atgaacctgc cacaattcaa
     2641 cgatccggag ctacacgccg aggaactggc agcccggcag aagcaaaagg aagccgagaa
     2701 acaggcggct actgaggttg gacaagaagc tggcgcggaa gcaacacctc aagcggagca
     2761 gatcagaagc gtcgatacca cctctgaagg agagaagccc acggagactc acgaggttgc
     2821 cgctaaaaac gaggaaacgc cccaggctgc tcaagatgaa ggtgcatcgg aaccgtccga
     2881 cagttaacag gagccataga tcgcgtccgt tgggacgatc atgaaatgat gagacagtaa
     2941 tcaaaggatt cgtatggaag attgcctgtt ctgcaagatt gtcgcgggcc agataccggc
     3001 gaagatcgtg taccaggacg aggaggttgt gtcctttgtg gacataaatc cgcaggcccc
     3061 gcagcacgtc ctggtgattc cgcgccgcca catcgcatcc atgcccgata tcaccggcga
     3121 agatggtccg ctgcttgcca gcatctttat ggcggctacg aagattgcgc gggaactcgg
     3181 cgtggcggag agcggcttcc gcgtcgtgac caatgtcggg ccggacgccg ggcaaagcgt
     3241 ctttcacctg cactttcacc tgcttggggg ccgcaggttg gcctggcctc cgggctaggc
     3301 cgcgccgcga gagagagcga catgcgtatt gcaatcgact atacggcggc gacccggcaa
     3361 gctgccggta tcagcaacta tgtgcgcagc ctggtagatg ctatgctcgc gcaggacacc
     3421 accaaccagt ataccctgtt gacgagcggg cgacctcccc aggatcgccc gtttcctgat
     3481 gcgaaaaacg tgcgcgggcg cagcctggtc attcccgacc gctacttgaa catcctgtgg
     3541 tatcgctggc gtctgccgct ctccgccacc ttcttcaccg gacaggtcga tctctatcat
     3601 ggccccgatt tcgtgctgcc tcccctcaac ggcaagctgc gtaagatcgt caccgtccat
     3661 gacctggcct tcctggaaca tccagaatac gctgtggcgg cgctggccgc ccacctcaag
     3721 aaggtcgtgc cagaggccgt agccgccgcc gatgccgtcg tggcagtctc aggggaaaca
     3781 cgccgcgcct tggtcgaaca attcgccacg ccgccccaaa agatcacact cgtgccgaac
     3841 ggcatccgtt cttccttgaa gcgcgtcacc aaccccattt tgcttgaagc ggcgcgtcac
     3901 aaatatggac tgaaaagccc gctggtgctg ggcgttggca cactcgaacc gcgcaaaaat
     3961 cacctggggc tgatcaaagc cttccacaag tccgccagtt ccgccaacaa gcaggcgcgt
     4021 cccgccatgc tggccattgc gggcggtgag ggctggatgt atgaggagac acgccaggcc
     4081 gtggcagacc ttaagctgga aaagaaggtg cgcttcctgg gccgcgtcag cgaccttgat
     4141 ctgatgctgc tctacagcct ggcggatgtt tttgtcttcc cctcgttcta cgaaggcttc
     4201 ggagtcccgc ccatcgaggc gatggcatgc ggtgcgcccg tcatcacctc gaacgtctct
     4261 tcgctgccgg aggtcgtcgg cgatgcagcg ctgctggtcg atccacacaa caacggcgcc
     4321 atcgcccagg ccatcctgcg cgtattgggc aacccgcagc aagcggagga actgcgccag
     4381 aagggatatg agcgcgccca acgcttcacc tgggccgcgt ccgccagaaa aatgctctct
     4441 atctatgcac ggctctatgc aggcaagaca accttcgatg aagaggtaac agaaacgcta
     4501 tgaaaattgg tttgaatgcg atgatgttcc gctttccggc gaccggcagc ggacaatatc
     4561 tcacccacct gataaacacg ctggccgaaa tcgaccagca aaacgaatat ctcttactgg
     4621 ggccggaccc cttccccgaa cagcagggca tcgatccgcg cctggcgtac cagcaggcat
     4681 tcgtccccgc gcctatccgg cagaatctgc gcctggaaaa gctggcgtgg gaacaatgga
     4741 ccgcgccatc gcaagcgcgc aaggccggcg ttgacctgct gcacgtccca tacttcgcgc
     4801 cgccctattt ctcgcgcctg cccgtcgtca ccaccattca cgatgtcatc cccatacgcc
     4861 ttgccgccta tcgctacagc ggttttatgc gagcgtacct gcgcatggtc gggcgcgccg
     4921 cgcacaaatc cacgctgatc atcacggtct cgcagcacgc caaacaggac atcatggacg
     4981 ctctcaatct gcccgccgag cgcatccgcg tgatctacca ggcggcgagc aaggaatacc
     5041 aacctatcac cgatgccgca gaactggcgc gcgtcaaggc gcgctacggt gtgggcagtc
     5101 gtttcatctt ctacctcggc gggctggatg cgcgcaagaa cgtcccggcg ctggtaagag
     5161 ccttcgcgca tgtctaccag cggctcggag atcctgacct gcaattgctc atcgcgggca
     5221 accccgataa gcagcgcggg ccgctcttcc ccgacccgcg cccactcgct gccgacctgg
     5281 gcatgtcggg gcagatcgtc taccgtttta tcgaagagga agacaagccc gctatgtata
     5341 gcgcagccaa tgtattcatc tttccctcgc tctacgaggg cttcggcctg acgccactcg
     5401 aggcaatgtg ctgcggcgcg ccggtggtct gctcgaaccg cacctcgctg ccggaggtgg
     5461 tcggcgatgc cgcgctgctc ttcgaccccg acaataccgg gcag
//
LOCUS       DASZAK010000765         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_161949, whole genome shotgun sequence.
ACCESSION   DASZAK010000765 DASZAK010000000
VERSION     DASZAK010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161949"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VGT44_13480"
     CDS             <1..650
                     /locus_tag="VGT44_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581859.1"
                     /translation="TDGGKTFTDHMVYDNPTTTVSYGHQFVNLSIDREGNLYSVFSDN
                     HSTFYSFSTSHATTWSAPVQVNGAGAATAIMPWSVANNAGHLDIVFYGTSYYDGVNPP
                     DNYPLSAAWYVFMVQNLKANKAGSTFTSVTATPIIHFGGTCEGGVTCTGNRDLYDDFG
                     VAANPITGLVSIIYSDDQYTNDANDPPRSGCTSSTSNSSSCDHTSIALQTGGPGI"
     gene            complement(724..1539)
                     /locus_tag="VGT44_13485"
     CDS             complement(724..1539)
                     /locus_tag="VGT44_13485"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2581860.1"
                     /translation="MLNDEMKSNRGWWDEAAQVHYQGEWYQMKDFRAGSIKLHPLERD
                     EVGDVAGKKLLHLQCHFGMDTLSWARLGAQVTGIDFSEEAIAIARGLSEEFKLDATFV
                     CCNLYDLPNLLEGAGTYDIVFSSYGAICWLPDLQPWGQIIARYLKPGGFFYLAEGHPF
                     LWSLDDQSSEFRVKYPYFSNEPIRDESSGTYADKDAALEHRVTYSWNHSLSTIFGSLL
                     SAGLTLDFFHEHQFCAWEALPGMEEGEDRLYRFKDTKLREMIPLMYSLKATKK"
     gene            1672..>2146
                     /locus_tag="VGT44_13490"
     CDS             1672..>2146
                     /locus_tag="VGT44_13490"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2581861.1"
                     /translation="MSQLIPIGRFAQITHLSVRALRIYANAGLLQPVYVDPESGYRYY
                     TLAQAARASRIRLLRLVDMPLEEIREVLQTPDPEAMRTHLTNHHQRITDRIARDQQSL
                     LLLQRVREQPDAFLSFTVKVKEVADQPLLSLCTHAASGTFGQAIRSALGALITYAR"
BASE COUNT          476 a          630 c          544 g          496 t
ORIGIN      
        1 cgaccgatgg cggaaagacg ttcaccgatc acatggtcta tgacaatccc accacgactg
       61 tcagttatgg ccaccagttc gtgaacctga gtattgaccg tgagggtaac ctctattccg
      121 tgtttagcga caatcacagc accttctaca gcttctcaac cagccacgcg accacgtggt
      181 cggcgcccgt ccaggtgaac ggcgcgggag cagctaccgc catcatgccg tggagcgtcg
      241 ctaacaacgc ggggcatctc gatatcgtct tttacggcac ctcgtattac gatggcgtga
      301 atcccccgga caactatcct ttgagcgcgg cctggtatgt ctttatggtg cagaatttga
      361 aggcaaacaa agcgggcagc acatttacct cggttacggc aacgccgatc atccatttcg
      421 gcggcacctg cgaaggcggc gtaacctgta cgggcaatcg cgacctgtac gatgatttcg
      481 gcgtggctgc gaatccgatc accggcctgg tatcgatcat ctacagcgat gaccagtaca
      541 cgaacgatgc gaatgatccg cctcgctccg gctgtacatc ctcaacttcc aactcgtctt
      601 cgtgcgacca tacctctatc gccctacaaa cgggcggccc cggcatctag cagctttgcc
      661 tcgctactgg ggcataggtg agcgtagtca aaagaatggg agctaagacg atggctccca
      721 ttcttatttc ttcgtggcct tcaacgagta catcagtggg atcatctcgc gcagcttcgt
      781 gtctttgaaa cggtagaggc gatcttcgcc ttcttccata cctggcagcg cctcccatgc
      841 gcaaaactga tgttcgtgga agaaatcgag agtcaatccc gccgagagaa gcgagccgaa
      901 tattgtgctg agggaatggt tccagctata tgttacccgg tgttccagcg cggcatcttt
      961 atcggcatat gtcccgcttg actcatccct gatcggctcg ttggagaagt aggggtattt
     1021 gactctgaat tcggaggatt gatcgtcgag cgaccacaga aatggatgcc cctcggcaag
     1081 atagaagaat ccgcccggtt tgagataacg cgcgatgatc tggccccagg gctgtagatc
     1141 gggaagccag caaatcgctc catacgagga aaaaacaata tcgtatgttc cagcgccttc
     1201 cagcaggttt ggaagatcat aaagattgca gcagacgaag gttgcatcga gcttgaattc
     1261 ctcgctcaac ccacgcgcta tagcgatggc ctcctcggaa aaatcaatac ctgtgacctg
     1321 cgcgcccaac ctggcccagg aaagcgtatc cattccaaaa tgacattgca ggtgcagcag
     1381 cttctttccg gccacgtccc ccacttcgtc tcgttccagt gggtgcagtt ttatcgatcc
     1441 tgctcgaaaa tccttcatct ggtaccattc gccttgatag tgtacctgcg ccgcctcgtc
     1501 ccaccagccc cggttgcttt tcatctcgtc gttcagcatg acattggcct cctttagcct
     1561 gttttaaggt attgtagcat ggtcgtgcca acccggactt ctccatcaac ttccctcttg
     1621 actcttcccc aggggaagac cctacaatga ttaagggtcg aaggaggaga catgtcccag
     1681 ttgattccca tcgggcgctt tgctcaaatt acccacctca gtgtcagggc gttgcgcatt
     1741 tacgccaacg cgggactctt acagcctgtg tacgtggacc ctgagtcggg atatcgctac
     1801 tacactctag cccaggcagc acgagcctcg cgtatccgtt tacttcgcct ggtcgatatg
     1861 cccctggaag agattcgaga ggtactccag actcctgatc cagaggcaat gcggacgcat
     1921 cttaccaacc accatcagcg gattacggat cggatagcca gggatcagca atcgctcctg
     1981 cttctccaac gggttcgtga acagccagat gctttcttgt cgttcaccgt gaaggtcaaa
     2041 gaggtagctg accagccgct cctcagtcta tgcacgcatg cggcctccgg caccttcggg
     2101 caggcgatcc gctcagcttt aggcgcattg atcacctatg ccagag
//
LOCUS       DASZAK010000766         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_195077, whole genome shotgun sequence.
ACCESSION   DASZAK010000766 DASZAK010000000
VERSION     DASZAK010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195077"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1167)
                     /locus_tag="VGT44_13495"
     CDS             complement(28..1167)
                     /locus_tag="VGT44_13495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2581862.1"
                     /translation="MKVALVHDYLNQMGGAERVVLAFHEMFPDAPLYTSIYDPNRVDA
                     AFRNIDIRTSFMQKLPLVKKHHQPFLPLYPFAMERLDLRGYDLILSSSSAFGKGVIVK
                     PGTMHICYCHTPMRWCWNYDEYIEREGLGRLPRAVLPFIITGLRVWDQTTAMRVDHFI
                     ANSPVVAERIAKYYRRESVVIPPPVEASRFLFDPTTQPEDYFLIVSRLMPYKRIDLAI
                     QACNALQLPLVIIGGGRDEARLKRLAGPTIRFMGRLSDDEVLHYYAHCRAFILPGEED
                     FGITPLEAQASGRPVIAYGAGGALASVVDGVTGRFFAAQTPESLSSALTDFDERDYDP
                     QTIRNHALEFDKPRFHRRMLQFIEAKMSAGNAREELRTPTSELVR"
     gene            complement(1167..2816)
                     /locus_tag="VGT44_13500"
     CDS             complement(1167..2816)
                     /locus_tag="VGT44_13500"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV2581863.1"
                     /translation="MANPEATGTPGVMGSGAPASPKAADVPMSPTSTGTTSQLTRTIS
                     KRRSYRRQWQAIETIALIILDGALVFASFLLAYYLRYKVFYNSQIIVNLRNNLFGSES
                     PTPHPALPRPDVLTPLSAFTSVQIWITIGLVAIFALRGLYRIRPTGSWFRQAWKIASS
                     STLGLTIVIAYYFVSQSASNSRLLVPFVWVTTIVVLCTFRLIISSAIGILYRLGVAET
                     RVLVIGSGTLGKLIMQYMAANPHLGYSVVGFLHDLHDLPDLSEPPGDFGRFKMLGTLE
                     DLNMVIRSMQIDEVIIALPSNLHEQSIRSVRLLERLGTSFKLVPDIYELNLSRIDMDT
                     IDGIPLINIKQASLNRLQQLVTRAVDVIVSILIMVIGLPFWLCIALAILLTSRGPVIY
                     RQKRIGLNGHPFFCYKFRTMYKDADKKLPDLLVYNEAQGPLFKIKDDPRITPVGKFLR
                     RTSFDEIPQFLNIIRGEMSLVGPRPPLPREVEEYDERQKVRLTITPGLTGLWQVRGRS
                     DISFDEGVLMDLYYIENWSLRLYFHILLRTIPAVLFSRGAY"
BASE COUNT          656 a          813 c          864 g          627 t
ORIGIN      
        1 agttccactg tctctacacc tttcgtccta tcgtactaat tcgctggttg gcgttcgtag
       61 ttcttcgcgc gcgttccctg cgctcatttt cgcttcgatg aactggagca tacggcgatg
      121 aaaacgcggc ttatcaaatt cgagcgcgtg gttgcgaatc gtctgggggt cgtaatcacg
      181 ttcgtcaaaa tcggtcagcg ccgacgaaag gctctccggc gtttgcgcgg cgaagaacct
      241 gcccgtcacg ccatcgacga ccgacgcgag cgcgccgccc gcgccatacg caatcaccgg
      301 gcgaccggaa gcctgcgctt cgagcggcgt gatgccgaaa tcttcttctc ccggtaagat
      361 gaatgcgcgg cagtgcgcat agtaatgcag tacttcgtca tcggagagtc gtcccataaa
      421 acggatcgtc ggcccggcca accgcttcag ccgcgcttcg tcgcgtccgc cgccgatgat
      481 gaccagcggc aattgcagcg cgttgcacgc ctggatcgcc aggtcgatgc gcttgtaggg
      541 catcaggcgg ctgacgatca ggaagtaatc ttctggctgc gtagtcgggt caaagaggaa
      601 gcggctggcc tcaaccggcg gcgggatcac caccgactcg cgccggtagt atttcgcgat
      661 gcgctcggcc accaccggcg aattggcgat aaaatgatcg acgcgcatgg ccgtcgtctg
      721 atcccagacg cgcagcccgg taataatgaa gggcagcact gctcgcggga gcctgcccaa
      781 tccttcgcgt tcgatatatt cgtcgtagtt ccagcaccag cgcatgggtg tatggcaata
      841 gcaaatgtgc atggtgcccg gtttgacaat tacgcccttg ccgaacgcgc tcgagctgct
      901 caagatcagg tcgtacccgc gcaggtcgag tcgctccatc gcaaagggat acaagggcag
      961 gaagggctgg tggtgctttt tcaccagggg caatttctgc atgaaggatg tgcgtatatc
     1021 gatattgcga aaagcggcgt ctacgcgatt gggatcatag attgatgtat agagcggcgc
     1081 gtcgggaaac atttcgtgaa atgccagtac aacgcgctct gctccaccca tctggttgag
     1141 gtaatcatgg actaacgcga ctttcatcag taagctcctc ggctgaagag aaccgccgga
     1201 atggtgcgta ggagaatatg gaagtagagg cgcagtgacc agttttcgat atagtacagg
     1261 tccatcagta cgccctcgtc gaagctgatg tcgctgcgac cgcgcacctg ccacagaccc
     1321 gttaaacccg gcgtgatcgt cagacgcacc ttctgccgct catcgtattc ctcgacctcg
     1381 cgtggcagcg gcgggcgcgg cccaaccagg ctcatctctc ctcgaataat attcagaaat
     1441 tgggggattt catcaaagct ggtacggcgg aggaacttgc caaccggcgt aatgcgagga
     1501 tcatccttga tcttgaagag cggtccctgc gcctcgttgt agaccagcag gtcaggcagt
     1561 ttcttgtccg cgtctttgta catcgtgcgg aatttataac aaaagaaggg atgcccgttc
     1621 aagccgatgc gcttctgtct gtagatcacc ggacccctcg aggtcaggag tatcgccagc
     1681 gcaatgcaga gccagaaggg caggccgatc accataatga ggatagagac gatgacatcg
     1741 acggcgcggg tcacgagctg ctgcaaccta ttcaaggaag cctgtttgat gttgatgagc
     1801 ggaatcccgt cgatcgtgtc catatcgatg cgtgacaggt tcagttcata gatatccggc
     1861 accagcttga aggatgttcc caatcgctcg agcaagcgca cgctgcgaat actctgctcg
     1921 tgcaggttcg acggcagggc gatgatgact tcgtcgattt gcatcgagcg aatgaccatg
     1981 ttgaggtctt ccagcgtccc cagcatctta aagcgcccaa agtcgcccgg tggctcgctc
     2041 agatcgggca ggtcgtgcag gtcatgcaaa aagccgacga ccgagtagcc caggtgcggg
     2101 ttggccgcca tgtactgcat aattaatttg cccaacgttc ccgacccgat gaccagtacg
     2161 cgcgtttcgg ccactcccag ccggtagagt atgccgattg ccgaggagat aattaaacgg
     2221 aaggtgcata acacgacgat ggttgtaacc cacacgaacg gcaccaacaa gcgcgagttt
     2281 gaggcagact gagacacaaa ataataggcg atcacaatcg tcaggccgag tgtggatgag
     2341 ctagcgattt tccacgcctg gcgaaaccag ctgcccgttg gacgaatgcg gtacagtccg
     2401 cgcagagcga aaatcgcaac caggccgatt gtaatccaga tttgtacgct ggtgaaagcg
     2461 ctaagcgggg taagcacgtc cggcctgggc aaggcgggat gtggggtagg actttcgctg
     2521 ccgaacaggt tgttgcggag attgacgatg atctggctat tgtaaaagac tttatagcgg
     2581 aggtaatagg cgagcagaaa cgaagcgaag accagcgcac cgtcaaggat aatcagtgcg
     2641 atggtttcga tcgcctgcca ctggcggcga tatgaacggc gttttgatat agttctggtt
     2701 agctggctcg tcgttcccgt tgacgtggga gacataggca cgtctgccgc cttgggagat
     2761 gcaggcgcgc cacttcccat cacgccggga gttcctgtcg cttccgggtt cgccacgatc
     2821 gtgtgaactc ctctgatgat ctcatccagt cgattgctct tcacaaatcc ctacctctat
     2881 atactaacgc gccagcgaag catcttgtgt ctgcctgagc aggctagact ggcctgccgc
     2941 ctgttcaaaa gccgcgatgg
//
LOCUS       DASZAK010000767         3844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_195108, whole genome shotgun sequence.
ACCESSION   DASZAK010000767 DASZAK010000000
VERSION     DASZAK010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195108"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..1167)
                     /locus_tag="VGT44_13505"
     CDS             complement(193..1167)
                     /locus_tag="VGT44_13505"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2581864.1"
                     /translation="MSQAAWPETNAFSPAPLANPATRAANIFELTRNELFKLWKRRLV
                     WGILLTDLVFVFLAWCVLVYYALKTGDGFTPGHLLGGVNGLSDALGQPMTLGRRAGEF
                     LVAALGGLAFGGEFSSGAIRLVLSRGVSRISYLVAKYIALAIACVALVVANLLMSMVL
                     VNFLIILHQPAPTLLNLNGPTLATTLSGCLGMLENFLFCLLLGATVSIIARSAAFGIA
                     ASFGYLIGEDIAAQILPVLGKSLHTPLGNQLVMLLLTPNLNAFYANTLPAFLAKGLDQ
                     LDGVIACTPVAKGCTTVGVGQSLLVPLIWGLVLCGISTYLFVRRDVLQ"
     gene            complement(1164..2156)
                     /locus_tag="VGT44_13510"
     CDS             complement(1164..2156)
                     /locus_tag="VGT44_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2581865.1"
                     /translation="MQYPGTQDRVAQEAWRMPLAAPTRDVVLQTIELTKAYGSGRSKR
                     LAVNGLNLEVLRGEVFGFLGPNGAGKTTTIRMLLNLIKPTSGEVRLFGQPLTGQSGVL
                     LPRVGALIEQPAFQSYLSGRDNLIAAGGYTGGVPASRVDEVLEVVGLDERGKDRYSTY
                     SLGMRQRLGVGAALLTSPELLILDEPGNGLDPAGIVEMRELVRTLAQQGKTIFVSSHI
                     LAEIRQVCTRIGIVRGGQLIGVGAVDELLRSTSRWEVEIANPEQVCRLLANVPIIRSL
                     TVENGVVVIQAPTMRGRDLIQYLGRNGIWPESVKRSEEDLEHIFLRLTDKEAGL"
     gene            complement(2294..2467)
                     /locus_tag="VGT44_13515"
     CDS             complement(2294..2467)
                     /locus_tag="VGT44_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581866.1"
                     /translation="MHIVDARFPRLPRLAKGQATIIDDLEELDGLIVKMSIACNAKEA
                     RRYLLDETEEDLD"
     gene            2689..3351
                     /locus_tag="VGT44_13520"
     CDS             2689..3351
                     /locus_tag="VGT44_13520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEV2581867.1"
                     /translation="MCGRFALTVDLADIAKKFGVEPTTQAMHSAPRYNVAPTQPVAAV
                     LRNGETHLAELRWGLIPSWAKDESIGARMINARAETLTEKPSFKGLLRSKRCMILADG
                     FFEWKQEPGGKTPMFITMADGQPFAFAGLWDSWKSPSGEPLRTCTIITTQPNELVATI
                     HDRMPAILLPEAYESWLDPSIKDEQLLSHWLAPYPAEAMMARPVSRLVNDPKNDRPEV
                     LS"
     gene            3420..3791
                     /locus_tag="VGT44_13525"
     CDS             3420..3791
                     /locus_tag="VGT44_13525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2581868.1"
                     /translation="MFTKIGTISIGVSDQDKALDFYVNTLGFEKIDDQPMSETERWLE
                     VAPPGAQTHIMLGLRGQSGGDKTGFTGYVLHTDNIEATCETLKARGVTLSKELNTEPW
                     GKWAQFTDLDGNEYGVWAPAW"
BASE COUNT          864 a         1127 c         1073 g          780 t
ORIGIN      
        1 gattgtccag cgagatttca ccttcttgtt ctaatcgaag aaatagatgc gaaagaggta
       61 tctttagcga ggagttttcc acaaattaga acatttgtat acatagttat ccacataaag
      121 ggacagcctt ccccgttgag gttcatagtt gcctcaacgg ggaaggctgt ccctttatgt
      181 gcagcgggta tgttactgca acacgtcccg cctgacaaac aggtacgtcg atatgccgca
      241 cagcacaagt ccccaaatta gcggcacaag cagcgactgc cccacgccga ccgttgtaca
      301 gcccttcgcg accggcgtgc aggcaatcac gccgtccagt tgatccagtc cctttgcgag
      361 gaaggccggg agtgtattgg cgtaaaaggc gttcaggttg ggggttagga gcaacatcac
      421 gagctggtta ccgagcggag tatgcagtga tttgcccagc acgggcagaa tctgcgccgc
      481 gatgtcctcg ccgatcaggt agccgaagct ggcggcgatg ccgaaggccg ccgagcgcgc
      541 gatgatgctg acggtcgccc ccagcagcag gcagaagagg aagttttcca gcatccccag
      601 gcagccggag agcgtggtcg cgagtgtcgg gccgttcagg ttcagcagcg tcggcgcggg
      661 ttggtgcagg ataatcagga agttgaccag caccatgctc attagcaggt tggcgaccac
      721 cagcgcgaca caggcgattg ccagggcgat atacttggcg accaggtacg aaatgcgact
      781 gaccccgcga ctgagcacca gccggatcgc gccgctgctg aattcaccgc cgaaggccaa
      841 cccgcccagc gcggctacga ggaattcgcc cgcgcgcctg cccagcgtca tcggctggcc
      901 gagcgcgtcg ctcagcccat taacgccgcc caacaggtgg ccgggagtga agccatcccc
      961 tgttttgagc gcgtagtaga ccagcacgca ccacgccagg aagacaaaga caaggtcggt
     1021 cagcagaatg ccccacacca ggcggcgttt ccacaatttg aacagttcgt tgcgcgtcag
     1081 ttcaaagata ttggccgcgc gcgtcgctgg attcgccagt ggcgcgggtg aaaatgcgtt
     1141 ggtttcgggc catgcagcct ggctcataat cctgcctcct tatctgtcag gcgcaggaag
     1201 atatgctcaa ggtcttcctc gctgcgtttt acactctccg gccagatgcc atttctgccg
     1261 aggtattgaa tcaggtcgcg cccgcgcatc gttggcgcct ggatcaccac gacgccgttt
     1321 tcgacggtga gcgagcggat aatcggtaca ttagccagca ggcggcagac ctgctccggg
     1381 ttggcgatct ccacctccca gcggctggtg gagcgcagca gctcatcgac cgcgccaacg
     1441 ccgatcaact ggccgccgcg cacaatgccg atgcgtgtgc aaacctggcg tatctctgcc
     1501 aggatgtggc tggagacgaa gatggtcttg ccctgctgtg ccagtgtgcg caccagctcg
     1561 cgcatctcga cgatacccgc cgggtcgagg ccgttgccgg gttcgtcgag aatcagcaat
     1621 tccgggctgg tcagcagggc tgcgccgacg cccaggcgct ggcgcatccc caacgaatag
     1681 gtcgaatagc gatctttgcc gcgctcgtcc agcccgacaa cctccagcac ttcatccacg
     1741 cgggacgccg gcacgccgcc cgtatagcca ccggcggcaa tcaagttatc gcgtccgctc
     1801 aaataagatt gaaaggcggg ctgctcgatc agcgcgccca cgcggggtag cagcacgccg
     1861 ctctgtcccg tcaatggctg cccgaagagc cgcacctctc ccgacgtggg cttgatcagg
     1921 ttaagcagca tacgtatcgt ggtggttttg cctgcaccgt taggtcccaa aaatccgaaa
     1981 acttcgccgc gcagcacttc cagattcaat ccattcaccg ccaaccgctt actcctccca
     2041 ctgccatagg ctttcgtcag ctcgattgtt tgcagaacaa catcgcgtgt gggcgctgcc
     2101 agcggcatac gccacgcttc ctgcgccacg cgatcttgcg taccgggata ttgcatcgta
     2161 gtcgtctctc cagatgtata tgcttggtag ccaggcaaaa aataggcagt gcctatcctt
     2221 gctatagtga tagacactgc ctgaaatgtg atgataacgg aaacgggatt attgtgctaa
     2281 tctatgtgac gatctagtcg agatcttcct ctgtctcatc gagcaggtaa cggcgggcct
     2341 cttttgcatt gcaagcaata ctcattttga cgatcaggcc atcgagttcc tcgagatcat
     2401 caataatagt tgcctgccct ttcgccaggc gcggcagcct gggaaagcgc gcatcaacga
     2461 tatgcaaaag cgtctaacgc agcgcccgtc catcgggaaa ctcccgtatc atgggagtct
     2521 ctgcaacctt cccatccctc tttaaatata ttactacaga ccaggcagag cagccgtact
     2581 gacgagttgc tttcagatta tactcccatt ttcgatcctc tcaaaaagaa tagattataa
     2641 tgagggtata tcaaacagtc ataaccaaac atcacaagga gcaaacatat gtgtggtcga
     2701 tttgcgctga ccgtcgatct ggcggatatc gcgaaaaaat ttggtgttga gccgacgacg
     2761 caggcgatgc atagcgcgcc gcgttacaac gttgcgccga cgcagccggt ggctgccgtg
     2821 ctgcgcaatg gcgagacgca cctggccgag ctgcgctggg ggctgattcc atcctgggcg
     2881 aaggacgagt ccatcggggc gcgtatgatt aacgccaggg ctgagacgct gacggagaag
     2941 cccagcttca agggattgct gcgctccaaa cgctgcatga ttctcgccga tggcttcttc
     3001 gaatggaagc aggagccggg gggcaaaacg cccatgttta tcacgatggc cgatggccag
     3061 cccttcgcct tcgcgggcct gtgggatagc tggaaaagcc cttcgggaga gccactccgc
     3121 acctgcacaa ttatcacaac ccaaccaaac gagctggttg ccaccatcca cgaccgtatg
     3181 cccgccattc tgctgcccga agcctatgaa agctggcttg atccatccat caaggatgaa
     3241 cagttgttgt cgcactggct tgccccatac cccgccgaag caatgatggc gcgccccgtc
     3301 tcgcgcctcg tcaacgaccc taaaaacgat cgcccggaag tcctttctta ggaagcttga
     3361 atttatgttc aatgtcagtt atcattgaac agagagtagt caacgaaaag gaggtattca
     3421 tgtttacgaa aatcggcacc attagtatag gtgtcagcga ccaggacaag gctctcgact
     3481 tctacgtcaa tacactcggc ttcgagaaga tcgacgacca gcccatgagc gaaaccgagc
     3541 gctggctcga agtggctcct cccggagcgc agacccatat catgcttggc ctgcgcggcc
     3601 agagtggcgg cgacaaaact ggttttactg gctacgttct gcataccgat aatatagaag
     3661 caacctgtga gacgctcaaa gctcgtggag tcacgctttc taaagagctg aataccgaac
     3721 cctggggcaa atgggcgcag ttcaccgatc tcgacggcaa cgaatacggc gtctgggcgc
     3781 ctgcctggta aattcccctt cacatttgat agagggccga tgcaaagttg tgcgaggcat
     3841 ggcg
//
LOCUS       DASZAK010000768         3950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_517680, whole genome shotgun sequence.
ACCESSION   DASZAK010000768 DASZAK010000000
VERSION     DASZAK010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517680"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2490)
                     /locus_tag="VGT44_13530"
     CDS             complement(<1..2490)
                     /locus_tag="VGT44_13530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581869.1"
                     /translation="MIKERVTHCECCGLLLEESTEECPLCRYPLDPEKEERFLLAAIE
                     DLQRAVTYGGSNLRVIDLLARYRVRLKTLHEKRSLPVPAAPPVAEAPRAEVAPPPVAA
                     PVPAPAAAPREVEAVVPVAQPPAPRRVFSWKAFLADQAINIVASLGAFLILIGALGFT
                     ATTSNLLLAFFIVFAVHAVFGITGSVTYRFASFRVVATIYSVIYVLLVPLVGFSAYRL
                     IAGNYIALSVPVLVAIAATYAASVYTLLAIYQRFPLFAYLGIAALAVADMAVASALAL
                     GYWWWPSMLMILALPSLVSVQQSADTLWLFAGNRAVLREPVRIFAYIFAGICALGVLT
                     IAGYSFLLDASGTPQTEVRYSLLSLTLLALLWSSLFLWLTRRPRSVIGLAFLFLVSVL
                     ALCYALVFEAIGYALALMAVATLYHGLSRFAPRLLQPFGTLERNLDWTALALVFLAPW
                     ISSPWLPVQLVVNAYALGEPSHTSWQTIAEFIALAVGLAITLSVTFKRAGLRRTPAQP
                     AWCWLLLLGGFLLDFAVALAIVSLNLTPTWSLLGVTLALMAGAVIVRQRAGAAWANPL
                     DIGVLVNIALTLGLAGNLGQDAISALLLFFAAATYSILLYQRRQNWLAVPLIFALLAL
                     PTLLSLRGVLLVASVTLPLAAIVVRRLVSNTWAAAHAKWLADVRLANVWEWPLLAAGL
                     IYGVTIATNDVGASTSTIQSWLGVPCPVAVELALLAFVWYGSAALARVKAWLLPATGF
                     AIGAALLPANMYWALVGIAPALAILAVVIRRLADREWALPLYITALLSGVMAGYTGFT
                     QDHLGAAAWVLLAFAILAYMIGAVEDELIPMW"
     gene            2990..>3950
                     /locus_tag="VGT44_13535"
     CDS             2990..>3950
                     /locus_tag="VGT44_13535"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV2581870.1"
                     /translation="MSTHHEIGHEQEILPGQAVIPGQPLPPSSRPPTRRRLLVALVTI
                     IIIVLLMAMSAGIVALARLNQPHVTPTPGPSATTAVTPTLVPTSTAQPTETPLPPGQW
                     VQVLTDYRVTQLVAAPSQPNVVYACAIAPGVPIEYRSVETVLRSADFGATWQDIGKRA
                     QMSRGCELAVNPTDSYEIVVATSSNPPADETVPSYVLEHTTNGGDTWETIHPMVEVPG
                     LNTAVAWQGMSLRFAGNRLWSLQAISTSLTPAPQGLQVAGRVITSVDGGHTWTVLDTR
                     LAASGRSAEAYAVALAQPAVVYELAYVPVVPGTAPPPLELYQSV"
BASE COUNT          859 a         1219 c         1258 g          614 t
ORIGIN      
        1 ccacatcggg atgagttcat cctcgactgc gccgatcata tacgccagga tcgcaaacgc
       61 gagcaatacc caggcggcgg cccccaggtg gtcctgggtg aaaccggtat agccggccat
      121 gacgccactc agcaaggcgg tgatatacaa tggcaacgcc cactccctgt cggcgagacg
      181 gcggatcacg accgccagga ttgccagcgc gggtgcgatg ccgaccagcg cccagtacat
      241 attcgcggga agcagtgcgg cgccgatggc aaagcctgta gcgggcagca gccacgcctt
      301 gacgcgcgcc agggcagccg agccatacca cacgaacgcg agcagcgcca gttctaccgc
      361 aaccgggcag ggtactccca gccaggactg gatcgtactg gtcgaggcgc cgacatcgtt
      421 tgtggcgatg gtcacgccgt agatcagccc ggcggccagc agcggccact cccacacatt
      481 ggccaggcgc acgtcggcga gccacttcgc atgggctgct gcccacgtgt tagaaacgag
      541 tcggcgcacc acgattgctg ccaggggaag cgtgacgctg gccacgagga gtactcctct
      601 gagcgacagc agggtcggca gcgccagcaa agcgaagatg agcgggacag cgagccagtt
      661 ctggcgacgc tggtagagca agatgctgta agtcgcggca gcgaagaaga gcaggagcgc
      721 actgatcgcg tcctggccaa ggttgccggc taagccgagc gtgagggcga tattcaccag
      781 cacgccgatg tccagcgggt tcgcccacgc tgcgcctgcc ctctgccgca cgatgaccgc
      841 gccagccatc aaggccagtg tcaccccaag cagcgaccag gtcggcgtga gattcaggct
      901 gacgatcgcc agggcaaccg cgaagtcgag caagaaacca cccagcagga gcagccagca
      961 ccacgcaggt tgtgcgggtg tgcgccggag gccggcgcgt ttgaacgtca cgctcagcgt
     1021 gatggcgaga cccacagcaa gcgcgatgaa ctccgcgatg gtctgccagc tcgtatgaga
     1081 cggttcgccc agcgcgtagg cattcacgac gagctgcacc gggagccagg gcgagctgat
     1141 ccacggcgcc agaaacacga gcgccagcgc ggtccagtcc aggttgcgct caagcgtgcc
     1201 gaagggctgc aggaggcgtg gggcgaagcg gctgagtcca tggtagagcg tggcgacagc
     1261 catcagcgcc agcgcatagc cgatggcctc gaacacaagt gcatagcata gggccagcac
     1321 actcaccagg aacaggaagg ccagcccgat gacggagcgt ggacgcctgg tgagccagag
     1381 gaagaggctg gaccagagca gcgcaagcag ggtcaggctg aggagcgagt agcgcacttc
     1441 cgtctgcggc gtacccgagg catcgagcaa gaatgaatag cccgcgatgg tgagcacacc
     1501 cagggcgcag attcccgcaa agatataagc gaagatgcgc accggctcgc gtaagaccgc
     1561 ccggttcccg gcgaaaagcc agagcgtatc tgcggactgc tgaacagaga ccagcgatgg
     1621 cagcgccagg atcatcagca tggagggcca ccaccagtag ccgagcgcga gggcgctggc
     1681 gacagccatg tctgcgacgg ccagggcagc gatgcccagg tacgcaaaca gcgggaaacg
     1741 ctggtagatc gccagcagcg tgtacacgct cgcggcatag gtggcggcga tggcgacgag
     1801 caccggcacg gaaagcgcta tatagttgcc ggcgatgagc cggtaggccg agaatcccac
     1861 caggggtacc agcaggacgt agatcacgct gtagatcgtc gcgaccacac ggaacgaggc
     1921 gaaacgatag gtgacagaac ccgtgatgcc gaaaacagcg tggacggcga acacgatgaa
     1981 aaacgccagc aggaggttgg aggtggtggc ggtaaaaccg agcgcgccaa tcaggatcag
     2041 gaacgcgccc agcgaggcca cgatgttgat ggcctggtct gccaggaacg ctttccagga
     2101 gaacacgcga cgaggagcgg gcggctgggc aacaggtaca acggcctcca cttcacgcgg
     2161 cgctgctgcg ggagccggga caggcgcagc aactggcggc ggggcgactt cggcgcgcgg
     2221 cgcctcagca accggcggtg cagcggggac aggcagtgac cgcttctcgt gcaaggtttt
     2281 caagcggaca cgatagcgcg cgagcaggtc aatgacccgc aggtttgatc caccgtaggt
     2341 cacggccctc tgcaaatcct cgatcgctgc cagcaagaag cgctcttcct tctcgggatc
     2401 gagcggatag cggcagagag ggcactcttc tgtggattcc tcaagcagca aaccacagca
     2461 ctcacaatgg gtgacacgtt ccttgatcat ggcccacctc ctgtgagcgg gtcctgcccg
     2521 cccaggttca gcatctctca gacacccggc ctgcgtatga tgccggcaga tcatccttct
     2581 atgtgtatga aagccaatct tccaatttac agtgtaatgt atgcacctca ccaccacatc
     2641 acagccgacg ggcaagaaca cacggtaccg tcacaaaatg ccatgacagg cattccttgc
     2701 agaggcaacc ctgtctccga ggccactata aaagctcctg acctatcaga acaggtttcc
     2761 agaaagcctt gtcgcaggaa tttgtgtcag gattgtgttg gcgaggcggc attttgtgat
     2821 ggcctggtga gagagaggcg ctattctcta ttttgggaga ttacgggcgg taagggtgaa
     2881 gcaaattggt tgcaccagtg gcgctgccct tctgctgccg cagtatccct tcccatgagt
     2941 ggtgaacatc tctcacttgt gtctggccgt ttcctccagg aggaaagtaa tgagtaccca
     3001 tcatgaaatt ggacacgagc aggagatact gccgggacag gctgtcattc ccgggcagcc
     3061 tcttcctcca tcctcaagac caccgaccag gcgccggttg ctggtggcgc tcgtgaccat
     3121 catcatcatc gtcctgctca tggccatgag cgctggcatc gtcgcgctgg cgcgcttgaa
     3181 ccagccgcac gtcaccccaa cgccaggtcc atcggcgaca actgcggtga cgcccacgct
     3241 cgttcccacc tcgacggcac agcctacaga gacgccccta cccccagggc aatgggtgca
     3301 ggtgctcacg gactaccgag tgacccagct cgtggctgcg cccagtcagc ccaacgtcgt
     3361 gtatgcctgc gccattgcgc caggggtgcc aatcgaatat cgcagcgtcg agaccgtctt
     3421 gcgcagcgcg gacttcggcg cgacctggca agacattggc aagcgcgcac agatgagccg
     3481 cggctgcgag ctcgccgtca atcccaccga tagctacgag attgtggtgg cgacgtcgtc
     3541 caacccgcca gccgatgaaa cggttccctc ctacgtcctc gaacacacga cgaacggcgg
     3601 ggatacctgg gaaacgatcc accccatggt ggaggtgcct ggcctgaaca ccgctgtagc
     3661 ctggcaggga atgtctctcc gctttgcagg caatcgtctc tggagtttgc aggcgatctc
     3721 tacttcactc acgccggccc cgcaggggtt gcaagtggcg gggcgtgtca tcacaagtgt
     3781 ggacggcggg cacacctgga cggtgcttga cacccggctc gctgcgtcgg ggcgctcggc
     3841 agaggcttat gcagtcgccc tagctcaacc agcagttgtg tacgaactgg cgtatgtgcc
     3901 ggtcgttcca ggcacggcgc ccccgccgct ggaactctat caaagcgtcg
//
LOCUS       DASZAK010000769         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_338864, whole genome shotgun sequence.
ACCESSION   DASZAK010000769 DASZAK010000000
VERSION     DASZAK010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338864"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..1236
                     /locus_tag="VGT44_13540"
     CDS             361..1236
                     /locus_tag="VGT44_13540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2581871.1"
                     /translation="MLQTEVQEIWLDVDGMRIHCLTAGQSGSPVVLLHGAGIDSATLS
                     WGDAIGPLSAHHRVFAPDLPGYGRSSKPDIQYTTDFYVSFVDHLLDVLHLEKISLVGL
                     SMGGAIALSFTLCFPDRVKKLVLVDSYGIIGADTWPRWVYLLTYFYVHHLSFLNELIY
                     RLIGASRNLVRWQLVALGLVHNAKRLSPQLIDQVYQHAHLPGRGKAYTSFQQSEFLWN
                     GLRTYLVDRLHEITVPTLIVNGEKDWGIPLIYAQKAHELIPGSQLYVLKDCGHWSQRE
                     MPEEFNRVVGDFLDA"
     gene            complement(1261..>1725)
                     /locus_tag="VGT44_13545"
     CDS             complement(1261..>1725)
                     /locus_tag="VGT44_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV2581872.1"
                     /translation="GARVIATTSSEEKAQRLKALGADDVVNYHTTPDWDITVRELTNG
                     RGVDCVVEVGGAGTLEKSIKSTTIEGQISLVGWLAKDKSTIDINVISSTVLTLRRIAV
                     GSRAQFIAMNRAIAVNRLKPVIDRVFPFDDVRAAYRYYEDGQYFGKVIISHS"
BASE COUNT          369 a          517 c          460 g          379 t
ORIGIN      
        1 tcggcttgca gatcgtgggg cgtcgcttcg ctgagaaggc cgtcctgcgc gcctcggcct
       61 gctttgaagc tgctcgtccc tgggcagatc gttggccagc actctaaagc tgctcgtttc
      121 tctgctgggt gaggcgtatc agcatcggat gttcatcacc cggcagaggt tgcacccata
      181 accatggtcc caggcccatg cctctctgat cactcgagag gaggaaacag gcactgcgag
      241 cgttcctgga ttcctggtac ccgcaagggg caccagtaca tttcctacga atcgatgatc
      301 gtcataaatg tagtggcggg gcttgccccc gcaaggctgc ggtaaaggag aggcgatacc
      361 atgctacaaa cagaggtaca agaaatctgg ctcgacgtcg acggaatgcg tattcattgt
      421 ctcactgccg gccagagcgg ctccccggtt gttctcttac acggcgctgg catagactca
      481 gcaacccttt cttggggcga tgccatcggg ccattgtcgg cccatcaccg tgtcttcgct
      541 cccgatttgc caggctatgg gcgaagctcc aagccggata tccagtacac gacggatttc
      601 tatgtcagtt ttgtagatca cctgctggac gtgctgcacc tggaaaaaat cagcctggtc
      661 ggcctttcaa tgggaggcgc aattgccctc agctttacgc tgtgtttccc cgaccgcgtt
      721 aagaagctgg tgctcgtaga ttcctacggc atcattggcg cggatacctg gccaaggtgg
      781 gtgtacctgt tgacatactt ctacgtccac catctctcgt tcctcaatga actcatatac
      841 cggctcatcg gtgccagccg gaacctcgtg cgctggcaac tcgtggcatt aggactcgtt
      901 cacaacgcaa agcgcctgtc tccacaacta atagaccagg tctaccagca cgcccatctc
      961 ccaggccggg gcaaagccta cacttccttc caacaaagcg agttcctgtg gaacggcctg
     1021 cgcacctatc tggttgatag gctccatgaa attactgtac cgacgctgat cgtcaacggc
     1081 gaaaaagatt ggggcatccc actgatctac gcacaaaaag cgcatgaact catcccaggc
     1141 tcccaactct acgtactgaa agactgcggg cactggtccc agcgtgaaat gccggaggaa
     1201 tttaaccgcg ttgtagggga cttcctcgac gcataaaatt ctgatccagc ggatcggcgc
     1261 tcaactgtga ctgatgatca ccttgccgaa atactggccg tcttcgtaat aacggtaggc
     1321 ggctcgaacg tcgtcgaaag gaaacacgcg gtcgatgacc ggcttcagcc tgttcacagc
     1381 gatagcgcgg ttcatggcga taaactgagc gcgactgcca acagcaatgc gccgcagcgt
     1441 gaggacggtg gaggagatga cgttgatatc aatcgtcgat ttgtctttgg cgagccaccc
     1501 gaccaggctg atctgtccct caatagtggt cgacttgatc gacttctcaa gcgtaccggc
     1561 gccgccgact tcgacgacac aatcaacgcc tcgaccattc gtcagctcgc gcaccgtgat
     1621 atcccaatcg ggcgtcgtgt ggtaattgac cacgtcatct gcccccagag ccttcagccg
     1681 ttgcgccttc tcctcgctcg acgtggtcgc aatgacacgc gcgcc
//
LOCUS       DASZAK010000770         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_54248, whole genome shotgun sequence.
ACCESSION   DASZAK010000770 DASZAK010000000
VERSION     DASZAK010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54248"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..913)
                     /locus_tag="VGT44_13550"
     CDS             complement(113..913)
                     /locus_tag="VGT44_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV2581873.1"
                     /translation="MTLPQAIILFIAAMLGGTLNAVAGGGSFISFPALIFTGVPPIQA
                     NATSTVALWPGSVASVGAYRKELAALNRVVVLLLGVTSLVGGVLGAILLLLTPQATFL
                     GLVPYLLLLATLLFAFSPRITALLRRRNSTSQGIESKASISRRTLTVIVLVQFVIAIY
                     GGYFGGGIGIMILASLGVMGMENIHEMNGLKTVLQTSINGVAVITFVIARAVFWLQAL
                     VMIAGAIVGGYGGAYVARKLDPRFIRGFVIVVGVGMTIYFFLHNFKVI"
     gene            complement(924..1823)
                     /locus_tag="VGT44_13555"
     CDS             complement(924..1823)
                     /locus_tag="VGT44_13555"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEV2581874.1"
                     /translation="MPKQWDDNIKRLIRANPQHYVTRVLKGAIYKDALSIELKNVTRE
                     ADFLLDVEYEGDSLLLLIEAQSYKDENMALRLPEYLILAIREHGRPILPVLIYLRKDL
                     KVPESPLILQAPVGHEVLRFHFIVLKLWAMKAEELIEAGLLGLLPLVPLTADGREYEV
                     IDRIVTELAEASEYDLLEYTRRFASLVFQDAANLEWLNRRFNMYKDILEDSWVGQEER
                     REGAVRGLHQTVIKLVKAHFPEIVELAKKQIDGIQNPEVLQDLILKISFAQNVQEAVQ
                     LLFAVDQRIGGADIDNDEPTVSD"
     gene            complement(1893..2993)
                     /locus_tag="VGT44_13560"
     CDS             complement(1893..2993)
                     /locus_tag="VGT44_13560"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2581875.1"
                     /translation="MLRDGDSSPTGVLQEEQATSLVYSVIDRDDPVLTRHLVETKLRE
                     MQRVGITTIHDIEDNDAFRLFQQLRDESALGVRVQMILPRRMLPELRAVGVRAGFGDD
                     LLRLGGIKIFADGTLGSQTAAMLDGFEGDPGNRGILVIPEQEMNEAVSAAADMGISIA
                     IHAIGDRASRVALNSIELAQRHLADLPVSPHYARLRYRLEHVQLIAPEDLERMRRLGV
                     VASVQPFHAVADRDIAERYWGKRHRRAYAYRTMLDMGIPLALGSDAPVETFDPLRILY
                     AATARNDTATRQRPPWLPDQSLPLTRALWAYTLGAAYAGAEEDSKGSLSVGKLGDAVV
                     LREDIMSAPPEKMPENGVQATILGGKVVYGEV"
     gene            2939..>3160
                     /locus_tag="VGT44_13565"
     CDS             2939..>3160
                     /locus_tag="VGT44_13565"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2581876.1"
                     /translation="MPALPARRPSGCCRRHAGSRRWPVQASLDRAAPEHPVTLWSKDG
                     HLLWVNSLAMERASLSAATPEPANGAILRD"
BASE COUNT          646 a          990 c          857 g          667 t
ORIGIN      
        1 gcaagcaccg ccagcactgc ccatttgttg gctggcttta tttcctcgcc ggtcgttcct
       61 cccaaggcaa cctgtgtttc gttcaactgc gccgcgcctg tgtcctgttg tttcatatca
      121 ccttgaagtt gtgcaaaaag aaatagatgg tcataccgac acctacaaca ataacgaatc
      181 cacgaatgaa gcgcggatca agttttctcg cgacatacgc gcccccgtat cctcccacaa
      241 tcgcgcccgc gatcatcacc agcgcctgca gccagaagac cgcgcgcgcg atgacaaagg
      301 taatcactgc tacgccgttg atgctcgttt gcagcaccgt cttcaacccg ttcatctcgt
      361 gaatgttttc catgcccatc acacccagcg aggccaggat catgatgccg atgccgccgc
      421 cgaaatagcc gccatagatg gcgatcacga actgcaccag cacgatcacc gtcaatgttc
      481 gccgcgaaat ggatgcttta ctctcaatac cctggctcgt actattgcgc ctgcgcagca
      541 gcgcggtaat gcgcgggctg aaggcgaaga gcagcgttgc cagcagcagc aggtagggaa
      601 ccagtcccag gaaggttgcc tgcggggtca gcagcagcaa aatcgcgccg aggacgcctc
      661 ccaccaggct ggtgacgccc agcagcagca caaccacgcg gttcaaggcc gccagttcct
      721 tgcgataagc gcccacgctc gccaccgaac caggccacag agcaaccgtg ctggtcgcat
      781 tcgcctgaat aggcggcaca ccggtgaaga tcagcgccgg aaaactaata aagctgcccc
      841 ccccggcgac cgcgttgagc gtcccgccca gcatagcagc aataaagaga atgatggcct
      901 gcggtagtgt catatgctat tgctcagtca ctcactgtag gctcgtcatt atctatgtca
      961 gcaccgccta ttctttgatc tactgcgaag agaagctgca ctgcttcctg aacgttttga
     1021 gcaaagctta ttttaaggat cagatcctgt agtacttccg ggttttgaat gccatcgatt
     1081 tgttttttcg ctaattccac aatctcagga aagtgcgcct tgaccagttt aataaccgtc
     1141 tgatgcaatc cacgaaccgc gccttctcgt ctctcttcct gcccgaccca ggaatcttcc
     1201 aggatatctt tatacatgtt aaacctcctg ttcagccact cgagattggc tgcatcttgg
     1261 aacacaagcg acgcgaacct acgggtgtat tctaacaagt catattcgct tgcttctgca
     1321 agctccgtca ctattctatc aattacctcg tattctcgcc catccgccgt tagaggaact
     1381 agaggcagca gccctagcaa gcctgcttcg atcaactctt ctgccttcat tgcccagagc
     1441 ttgaggacga tgaagtgaaa acgtagcact tcatgcccga caggcgcttg taagatcagc
     1501 ggcgactccg gaacttttaa gtcctttctc aaatagatca gaacgggcaa tattggtcgt
     1561 ccatgttcgc gaatcgcaag aatgagatac tccgggaggc gcagagccat gttttcatct
     1621 ttgtagctct gcgcctctat caataacaga agcgaatcgc cctcatattc cacatcaagc
     1681 agaaaatccg cttcgcgcgt cacgttcttc aattcgatcg aaagtgcgtc tttgtaaatt
     1741 gctcccttga gtacccgggt cacatagtgc tgcgggttag cgcggataag ccgcttgata
     1801 ttatcatccc actgtttcgg catagcacag gaccctttcc acgtatttta ttctgtttga
     1861 agtgtagcat actggaaaga gcctgtcgag cgttacacct ccccatacac caccttcccg
     1921 cccaggatcg tcgcctgcac cccgttctcc ggcatcttct ccggcggcgc gctcatgatg
     1981 tcctcgcgaa gcacgacagc gtcgcccagt ttgccgaccg acagcgaccc tttgctgtcc
     2041 tcctcggccc cggcgtaggc cgcgcccaac gtgtaggccc acagcgcgcg cgtgagcggc
     2101 agagactggt cgggcagcca gggcgggcgc tgcctcgtcg cggtatcgtt gcgcgcggtg
     2161 gcggcgtaga ggattcgcag cgggtcgaag gtttcaaccg gcgcgtctga gccgagcgcg
     2221 agcgggatgc ccatgtcgag catcgtgcga taggcatagg cgcggcggtg gcgtttaccc
     2281 cagtagcgct cggcgatatc gcggtccgcg acggcgtgga agggctgtac cgaggccacc
     2341 acgccgagcc gccgcatccg ctccaggtct tctggcgcga tcaactgcac atgttcgaga
     2401 cgatagcgca ggcgcgcgta atgcggactg acgggcaggt cggccaggtg tcgctgtgcc
     2461 agctcgatgg agttgagcgc cacgcgggac gcgcggtcgc cgatggcgtg gatggcgatg
     2521 ctgatgccca tgtcggctgc cgcgctgacc gcttcgttca tctcctgctc cgggataacc
     2581 agtatgcccc ggttgcccgg gtcgccctcg aagccgtcga gcatggcagc ggtctgcgaa
     2641 ccaagcgtcc catcggcgaa gattttgatg ccgcccagcc gtagcagatc gtctccgaac
     2701 ccggcgcgca cgcctaccgc acgcagttcc ggcagcatcc tgcgcggcaa gatcatctgc
     2761 acgcgcacac ccagcgcgct ctcgtcgcgc agttgctgga aaagacggaa ggcatcgtta
     2821 tcctcgatat catggatagt agtgatgcca acgcgctgca tctccctgag cttcgtttcg
     2881 acgagatggc gcgtcaacac cgggtcgtcg cggtcaatca cactgtagac cagcgaggtt
     2941 gcctgctctt cctgcaagac gcccgtcggg ctgctgtcgc cgtcacgcag gatcgcgccg
     3001 ttggccggtt caggcgtcgc tggaccgggc cgcgccggag catcctgtga cgctgtggag
     3061 caaggacggc cacctgttgt gggtcaattc gctggcaatg gaacgcgcct cgctttccgc
     3121 cgccacgccc gagccggcca atggcgcgat cctgcgtgac
//
LOCUS       DASZAK010000771         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_551576, whole genome shotgun sequence.
ACCESSION   DASZAK010000771 DASZAK010000000
VERSION     DASZAK010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551576"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1955)
                     /locus_tag="VGT44_13570"
     CDS             complement(48..1955)
                     /locus_tag="VGT44_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV2581877.1"
                     /translation="MWTSTMELSPRRVLLTRLLGQFWRGAYFSSFAPLQVQNLPRPTL
                     PARDWIRVRNRLAGICGSDLHFIAADGDFRIAPAALPAHKRSYPGHEVVGEVIEVGED
                     VQRVKVGDRVTLQYGQNCVSQGIQPLCRSCAAGQYSLCEQGTFSGQEQIGGGWSEEML
                     LHESQVFRVPDELSNEQAVLIEPTAVALHAVLRRLPQPGDNVLIVGSGTIGLLTLQIV
                     RALAPQATVSMMARYPFQVEQATRMGAEHIIYPQDTYKEVEQATGAKLYRGMMGNTML
                     LGGYDVIFDTIGTRHTLHDALRWTRAGGAVVIIGVSLHMMRLDLTPVWYQEVSLIGSM
                     SHGIEQWPLGSQERYSTFAIAAELMAQGLIHPDKLITHHFALTDFPEALMTAAAKTRT
                     RAIKVVFDYDKMPASVVPNVRSSARLRRPARSTTTTTGEAQVTEEAQEQPYQQIQPPA
                     TVEPAQAEHEWDHLDMPSGGTWAQQVNPFPSTPPLERDSSEPVLSTDYEPEPTIPQHL
                     LALTKPVEEPADDDELLPMDYAQSFSISEAFPLSSTPTEAMAEPLTTTYEQPFAVTEE
                     PTLSIPREPQPILLPNLASTDDKVGQADELQVEPTVSNQLTRQSRQRQRSAPRKTPDQ
                     PGATTEPTETL"
     gene            2323..2772
                     /locus_tag="VGT44_13575"
     CDS             2323..2772
                     /locus_tag="VGT44_13575"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEV2581878.1"
                     /translation="MFITRRVLIIIGAAVVVAVGLTVFAFIFFLSQAHQTAASSPTPT
                     PTLTAPTKAAQHPCATGTVASINSSASSFVVTKGAKSVTITVDGTTTFRSHGKQVTVS
                     SLAVGDQVRVVAQGTCAKTAQTFAAQVVMIVPKTVSPALTPTASPTP"
BASE COUNT          548 a          848 c          860 g          648 t
ORIGIN      
        1 cgacgcatgc gtcgcctccc tccacctccc ttcccccgcc cctacaatta caacgtctcg
       61 gtgggttcgg tcgtcgcgcc gggttgatcg ggtgttttgc ggggagcaga gcgctggcgc
      121 tggcggcttt gcctggtcaa ttgatttgag acggtcggct ctacttgcag ctcatcggcc
      181 tgccctactt tatcatctgt actggcaagg ttgggcagca aaatgggctg cggttcgcgc
      241 ggtatggata gcgtgggttc ttctgtgacg gcgaatggct gctcgtatgt cgtggttaat
      301 ggttcagcca tcgcctcggt aggcgttgat gacaggggga aagcctcact aattgagaag
      361 gactgcgcgt aatccattgg cagcagctca tcgtcgtcgg cgggttcctc gaccggcttg
      421 gtaagcgcga gcaggtgttg aggaatagtc ggttcaggct cataatcggt agagaggacc
      481 ggttcgctgc tgtcgcgctc tagcgggggg gtggatggga atggattgac ctgctgcgcc
      541 caggtgccgc cggatggcat gtccaggtga tcccattcat gctctgcttg cgccggttcg
      601 accgtcgccg gtggctgaat ctgctggtag ggctgctcct gggcttcctc ggttacttgc
      661 gcctcgcctg ttgtggttgt ggtggagcgg gcaggacggc gcaggcgcgc ggacgagcgc
      721 acgttgggga cgaccgaggc gggcattttg tcataatcaa agacgacttt gatggcgcgc
      781 gtgcgcgttt ttgcggcggc ggtcatcaat gcttcgggga aatcggtcag ggcgaagtga
      841 tgcgtaatca gcttgtccgg gtggatcagt ccctgcgcca tcaactcggc ggcgatggcg
      901 aaggtcgagt agcgttcttg cgagccaagc ggccattgtt cgatgccatg gctcatcgaa
      961 ccgatcaggc tgacttcctg gtaccagacg ggtgtgagat cgaggcgcat catatgcaga
     1021 ctcacgccga tgatcacaac cgcgccgcct gcgcgcgtcc agcgcagcgc atcgtgcagt
     1081 gtgtgccggg tgccaatcgt atcaaagatc acatcatagc cgccaagcag catcgtgttg
     1141 cccatcatcc cgcgatagag cttcgcacca gtcgcctgtt ccacttcctt gtaggtatcc
     1201 tgcggataaa taatgtgttc ggcgcccatg cgggttgctt gctctacctg gaacgggtag
     1261 cgcgccatca tgctgaccgt cgcctgggga gccagcgcgc gcacgatctg gagcgtcaac
     1321 agcccgatgg ttcccgatcc cacaatcagg acgttgtcgc ccggttgggg caggcggcgc
     1381 agtaccgcgt gtagcgctac cgcagtcggt tcgatcaaga ccgcctgctc gttgctcaat
     1441 tcgtccggca cacgaaagac ttgcgactcg tgcagcagca tctcctcgct ccaaccgccg
     1501 ccgatctgct cctgcccgct aaacgttccc tgctcgcaca ggctatactg ccccgccgcg
     1561 cacgagcggc agagcggctg tatgccctgg gagacgcagt tctggccgta ctgcagggtc
     1621 acgcggtcgc ccactttgac gcgctggaca tcttcgccga cctcaatcac ctcgccaacc
     1681 acctcgtggc cgggatagct gcgtttatgt gcgggaagcg cggctggcgc gatgcgaaaa
     1741 tcgccatcgg cagcaataaa atggagatcg ctgccgcaga tgccggcgag tcggttgcgt
     1801 acacgaatcc agtcgcgagc gggcaacgtg ggccggggaa gattttgcac ctgcaacggc
     1861 gcaaaggagg agaaatacgc gcctcgccag aattgtccga gcaaccgagt gagcagcact
     1921 cgcctggggc tgagttccat cgttgatgtc cacatggcct tatgctcctt tcatgcgcta
     1981 acagcttctt cgatccaccc tacccctttt cagcatttca tacaatcaaa cgtatcgcct
     2041 gtggcataat gtcgtgtcta ccggagtaca aattagcttg tgcaacttct tgcgccgcaa
     2101 gtgtatcacg cctgccggca atggtcaaat gtatggtact cagctatgtc aggatcggag
     2161 ggcgcaccct tgcggtcgcc ctccgatccc catttgtccc aaaagaatga cttttctgca
     2221 atgacatatt agaatgtagc ctatattcta gagaatagct tgccggatgc gttgaacgcc
     2281 ttcttgcgaa tgctgtccgg tacatacata aaggagcata aaatgtttat cacgagacga
     2341 gtgcttatca ttattggggc agcggtggtg gtggcggttg gtctcaccgt ctttgccttc
     2401 atatttttct tatcgcaggc gcatcagact gcggccagtt cgccgactcc cactcctacc
     2461 ctgactgcgc ccaccaaagc ggcgcagcat ccctgcgcga ctggcaccgt cgcctccatc
     2521 aattcctcgg catcctcctt cgttgttacc aagggcgcta agtcggtgac aatcacagtc
     2581 gatggcacaa ccactttccg cagtcatggc aagcaagtca ccgtgagcag cctcgccgtt
     2641 ggcgaccagg tgcgggtcgt ggcgcaggga acatgcgcta agacggcgca aaccttcgct
     2701 gcccaggtcg tcatgattgt gccgaaaact gtctcgccag ccctcacccc gacggcttcg
     2761 cccacgccat aacggtactc ttgccagaac tgccgtgttg ctctatcata tgtgaaggat
     2821 aatggactcc gcaggtgcgc gataaatcgg cacctactac atagtcaaag ttgatgtggc
     2881 gtgtaggggc gcaattcatt gcgc
//
LOCUS       DASZAK010000772         6819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_338888, whole genome shotgun sequence.
ACCESSION   DASZAK010000772 DASZAK010000000
VERSION     DASZAK010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6819
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338888"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1405
                     /locus_tag="VGT44_13580"
     CDS             41..1405
                     /locus_tag="VGT44_13580"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEV2581879.1"
                     /translation="MKSVRIAGGLGFYGDSWRPIKASIERGNVQYVASDHLAELTLAI
                     LQKDRQRDPSLGFTRDFAPMLLDLLPLAMPRGVKFILNAGGLNPLAAREALLAMLKKA
                     NIKLKIGVVLGDAVLERLDELQAAGVSLAHMDTGEALATVRERLVFASAYLGARPLVE
                     ALDAGADIVLTGRVADAALFLAPVVHELGWRWDDWDKLAQGIVVGHLLECSGQATGGN
                     FGGDWRAMPDLAHIGYPIAEVWESGEAVISKAPATGGRVSFDTLREQLLYEVHNPAHY
                     LTPDVDVNMTTLRMEEIGPDHVRITGATGQPAPPTFKIVAGYEDGVMGQATLGYAWPD
                     ALAKAQAAADIIQQQMREIGLQTDEVLVEYLGYNSIHGPLADPAHADDLNEVYLRIAV
                     HCPNRQEAAKLGRLFPPLALSGPPFIGGAGGMTDPRGLLGIWPTLAPRDLIEEYVRVS
                     VEES"
     gene            1411..1722
                     /locus_tag="VGT44_13585"
     CDS             1411..1722
                     /locus_tag="VGT44_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581880.1"
                     /translation="MKKRLVELAHARSGDKGNIANIALFAPDRATYDLLAREVTPERV
                     RAHFEGLVTGNVERYEVPNILALNFVLHDALGGGASRSLRSDALGKSMSSALLRMEIE
                     V"
     gene            complement(1700..2764)
                     /locus_tag="VGT44_13590"
     CDS             complement(1700..2764)
                     /locus_tag="VGT44_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581881.1"
                     /translation="MKLALKMTPQRSAQYANMTEKLAAPELLASPLAPSIRAVEPVTL
                     AGQPYLLVTLEESEVTGTNRHIAPSAMFDILARLGATSEAYEYFERIGELSGSFLRPI
                     DYTFEPFVPVEMAEARRYKGKTNEIFTRVLINTALFAGAFREQITGRLRILDPLAGGG
                     TTLFLALATGYDAFGIEQDRQDVESTALFVRQYLNGMGIPYKERDERGRRAGRRFQFE
                     IGKPGEKAGKGETRALVLAHGDTSQANAHLLEVPGGPHMHAIVADLPYGIQHYGEIAA
                     LLADALPAWQRLLLPGGTMALSWNATRIERDEIIQCFERYTDLRICNEPPYTQFAHTV
                     DRVIKKRDIIVAVATPQSPS"
     gene            complement(2771..3760)
                     /locus_tag="VGT44_13595"
     CDS             complement(2771..3760)
                     /locus_tag="VGT44_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2581882.1"
                     /translation="MHQSRTWLDLLREVIENSAERQRIAGILGVNPVTLTRWIEKRAR
                     PRAQNRQKLIDAMPAHMRQEFATLLAQEFPEEMRRSTEAEEDPIADIPSAFYARTFDA
                     YTSTPPSLRSSSIITIILQQMLAHLDVEQEGLFISVIRCTRPAPGQAVRSLQEVIGRG
                     TAPFKPYQGLQQSFLGAESLVGSAVMQCARRVNQNLRATSYAPALRTELEESAMACPF
                     TFEDRVAGGLLVSSTRMNFFTPKHQRLVQEYTNLLVLAMRPEDFYEQSHIDLALMPLE
                     REQQPYFQKFRRCVADIMHRPGNEALPESEAEIVAYQEIEREIVQQTNEQKVI"
     gene            complement(3993..>6819)
                     /locus_tag="VGT44_13600"
     CDS             complement(3993..>6819)
                     /locus_tag="VGT44_13600"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2581883.1"
                     /translation="ALPRHPLVMLSYYDGGQVGTLPPYEIEALAITNLLAAGAGAVLA
                     MRWPVASPRAREFAVLLYQELADGISQGEALRRARSTMAQRHPDDPSWLCYVLYGNPT
                     STLVTAPAGGRDRNLELPLDSLEDDHLLPSVLSSNALDRRFLQEVLGIALTEARRMHK
                     DYLGTPHLFIALTKLDGGCTQDALRNLNFSPKQVRDVIRIALGNGKATSDTPILPTRR
                     CKEILQTAEGNALKAGSSSIDERAIAQAVLSERDGVTYELLTKMGINPSHLIDTVMNS
                     NARAILELVPSVSANPAAAPIELPVGIGPEGGMKSGNSTLERLGRDLTKQASMKQLPP
                     LIGRENEMRLLIQTLMLKDRNNPILIGDSGVGKTTIVTGLAQRIVDGKVPLELRGKRL
                     IELSASSLVAGTKYRGEFEERLLKVLEEAEGSGDIILFIDEMHLLIGTGRAADGSIDA
                     AGILKPALAGGRLRCIGATTPQEYRTIEKDAAMERRLRPIQIEEPSEEEALAILTGMR
                     DLYEQHHRVEIAKDTLYAAVHLSVQYLPNLRLPDKACSVLDEACSQARVDLAEENENA
                     NEDELDDVFIDPPLIIPPMIAEVISRRTGIPVRAPGREERDRLLNLEVLLKRRVIGQD
                     AAIARVTQAIQVSRAGLKQRHRPSGVFLFLGPTGVGKTELARALAAEVFGSDEHLIRV
                     DMSEYMEKHAVSRMVGAPPGYIGHDQEGQLTGKLRRRPHCVVLLDEVEKAHPEVFDLF
                     LQVFDAGRLTDAQGHTVDAQHAIWIMTSNVGTDMLGRSMPSGFHAGKNAAEEMQRDRL
                     MERLRQTFRPEFLNRIDEVVIFQPLGQEQARAITRLQMNELATRLLEQGLTLDADDSA
                     VDLLCKEGFSQTQGARPLRRVIEKMLTVPLSLRILLGNIPDQAVVQVSAVEGKLAIDI
                     REPQEEPLDEEVEEDITVENEVKVDAV"
BASE COUNT         1276 a         2083 c         2090 g         1370 t
ORIGIN      
        1 ctgcctctcc ctgtccctac gaattataaa aggataggct atgaaatcgg ttcgtattgc
       61 cgggggactt ggcttctacg gcgactcctg gcgacccatc aaggccagca ttgagcgcgg
      121 caacgtccag tatgtcgcct cggatcacct ggctgaattg acgctggcca tcctccagaa
      181 agatcgccag cgcgacccca gcctgggctt cacccgcgac ttcgccccca tgctgctcga
      241 cctgctgccg ctcgccatgc cgcgcggcgt caagttcatc ctgaacgcag gcggcttgaa
      301 cccattggcg gcgcgcgagg cgctgctggc catgctgaag aaggccaaca tcaagctcaa
      361 gattggtgtc gtgctgggcg atgcagtgct ggaacgcctc gacgagctgc aagcggcggg
      421 cgtttcactg gcgcacatgg atacaggcga ggcgcttgcg acggtgcgcg agcggctggt
      481 gttcgcaagc gcctatctcg gcgcgcgccc cctggtcgag gcgctggatg ccggagccga
      541 catcgtgctg accgggcgcg tggccgatgc cgcgcttttc ctagcgccgg ttgtacacga
      601 gttgggctgg cgctgggatg actgggacaa actggcgcag ggcatcgtcg tcggccacct
      661 gctggagtgc agcggccagg ccaccggcgg caacttcggc ggcgactggc gcgccatgcc
      721 cgacctggcg catatcggct atcccatcgc cgaggtatgg gagagcggcg aggccgtcat
      781 cagcaaagcg cccgctaccg gcgggcgcgt cagcttcgat acgctgcgcg agcaactgct
      841 gtacgaggta cataaccccg cgcactatct gacaccggat gtcgatgtca acatgacgac
      901 gctacgcatg gaggagattg ggccggatca cgtgcgcatc actggcgcga ccggccagcc
      961 cgcgccgccg accttcaaga tcgtggccgg gtacgaagac ggcgtgatgg gccaggccac
     1021 gcttggctac gcctggcccg acgccctggc gaaggcgcag gccgcagcgg acatcatcca
     1081 gcagcagatg cgcgaaattg gcttgcaaac cgacgaggtg ctggtcgaat acctggggta
     1141 caactctata cacggcccac tggccgatcc cgcacacgcc gatgacttga acgaagtcta
     1201 cctgcgcatc gcggtacatt gccccaaccg acaagaagcc gcgaagctgg gccgcctctt
     1261 cccgcctctg gccttgagcg gaccgccctt catcggcgga gccggcggca tgaccgaccc
     1321 gcgcggtctg ctcggcatct ggccaaccct ggccccgcgc gacctgatcg aagaatacgt
     1381 gcgcgtcagc gtggaggagt cctgataacg atgaaaaagc gacttgttga actggcccac
     1441 gcgcgttccg gcgacaaggg caatatcgcc aacatcgccc tcttcgcgcc cgaccgcgcc
     1501 acctacgacc tgctcgcgcg cgaggtcacg ccagagcgtg tgcgcgcaca cttcgaggga
     1561 ctcgtaacag gaaacgtcga acgctacgaa gttccaaata tccttgccct caatttcgtc
     1621 ctgcacgacg ccctcggcgg cggcgcatcc cgttccttgc gcagcgacgc cctgggaaaa
     1681 tcaatgtcct ccgccctcct caggatggag attgaggtgt agcgaccgct acgatgatat
     1741 ctcgcttctt tatcacgcgg tcaacggtat gcgcgaattg cgtgtagggt ggttcgttgc
     1801 aaatgcgcag gtcagtatat cgctcgaaac actgtattat ctcgtcgcgt tcaatgcggg
     1861 tagcgttcca ggacagcgcc atcgtacccc ccggcaagag taggcgctgc caggctggca
     1921 aagcatccgc cagcagcgcg gcaatctcgc cgtagtgctg gataccatag ggcaggtcgg
     1981 cgacgatggc gtgcatatgc gggccgcctg gcacttcgag aaggtgcgcg ttggcctggc
     2041 tggtatcgcc atgcgccagc acaagcgcgc gcgtctcccc ttttccggcc ttctcgccgg
     2101 gcttcccgat ctcgaactgg aagcggcgtc cagccctacg cccgcgttca tcacgctctt
     2161 tatatggaat gcccatgcca ttcaaatact ggcgcacgaa gagcgcggtt gactccacat
     2221 cctgccggtc ctgctcgata ccaaaggcat cgtagccagt cgcgagcgcg agaaaaagcg
     2281 ttgttccccc acccgccagt gggtcgagga tgcgcaatcg ccccgttatt tgctcgcgaa
     2341 aagcgcccgc gaagagcgcg gtattgatca ggacacgcgt gaagatctcg ttggtcttgc
     2401 ctttatagcg cctggcttcc gccatctcca cgggaacaaa tggctcaaag gtatagtcta
     2461 tgggacgtaa aaaggagccg ctcagttcgc cgatccgctc aaaatactcg tatgcctcgc
     2521 tggtggcccc caatcgcgcc aggatatcaa acatggcgga aggcgcaata tgcctgttcg
     2581 tgcctgttac ttcactctct tcaagcgtca ccaacaggta gggctgaccg gcgagcgtca
     2641 ctggctccac agcgcggatc gagggcgcaa gtgggctggc aaggagttct ggcgcggcca
     2701 gtttctcggt catgttggca tactgagcgc tgcgctgcgg agtcattttc agggcaagtt
     2761 tcatgactgc ctagatgact ttctgctcgt tcgtttgttg tacgatctca cgttcaattt
     2821 cctggtaggc gactatctct gcctcgctct ccggcagcgc ttcattgccg gggcggtgca
     2881 tgatgtcggc aacacaccgg cgaaactttt ggaagtaagg ttgctgctcc cgttcgagtg
     2941 gcatgagggc aagatcaatg tgcgactgct cgtaaaagtc ttcgggacgc atggccagta
     3001 ccagcaggtt cgtgtattct tgcaccaggc gctgatgttt gggcgtaaaa aagttcatgc
     3061 gcgtactgga gaccagcagg cccccggcga cacggtcttc aaaggtgaat gggcaggcca
     3121 tcgcgctctc ttccagctcg gttcgtaatg ctggcgcgta actcgtggcg cgaagattct
     3181 ggttaacgcg cctcgcacat tgcataactg ctgacccgac caggctttcc gcgccgagga
     3241 acgattgctg caatccctgg tagggtttga aaggagcggt accgcgcccg atcacctcct
     3301 gcaagctgcg taccgcctgg ccaggcgcag gccgggtaca gcggatcacc gaaataaata
     3361 gcccttcctg ctctacgtca aggtgcgcca gcatctgctg taaaataatc gtaatgatcg
     3421 aagaggagcg caaggaaggt ggcgtcgaag tatatgcatc aaaggtacgc gcgtagaacg
     3481 cagacgggat gtctgcgatg gggtcctcct ccgcctctgt tgagcgccgc atttcctctg
     3541 gaaactcttg cgccagcaaa gtagcaaatt cctggcgcat atgcgcgggc atagcgtcga
     3601 tcagcttctg acgattctgc gcgcgtggcc tcgccctttt ttctatccag cgtgtgaggg
     3661 tcacaggatt gacgcccaga atgcccgcga tacgctggcg ttcagcagaa ttctcgatga
     3721 cttcgcgtaa aagatcaagc catgtccgtg actgatgcat tgtccttgct tcttgcacaa
     3781 aaataaagcc tgataatgac ttttagagca gcaaacgagg gttaatggaa gggttgcacg
     3841 ttctgcattt ttatcatacc ctatcgccgc ttgatagtca ataaaaattt cgataatttt
     3901 cactcaatca tatcaggaat caagcatgca ggtcacaatt gggatgaaat agccgcctgc
     3961 catgtcttca taaaagtaaa ctacgcggca ggttacaccg cgtccacctt tacctcgttc
     4021 tctaccgtaa tatcctcttc cacctcttca tcgagcggct cctcctgcgg ttcgcggata
     4081 tcgatggcaa gcttgccttc tacggcgctg acttgcacga cggcctggtc ggggatgttg
     4141 ccgagcagga tgcgcaggct cagcggtact gtcagcatct tctcgatcac gcggcgcagt
     4201 ggacgcgcgc cctgggtctg gctgaaccct tctttacaga gcagatcaac ggcgctatcg
     4261 tcggcgtcga gcgtcagtcc ttgctccagc aggcgcgtgg ccagttcgtt catctgcaag
     4321 cgcgtgatgg cgcgcgcctg ttcctggccg agcggctgga aaatgacgac ttcatcgatg
     4381 cggttgagga actccgggcg gaaagtctgg cgcaggcgct ccatcagccg gtcacgctgc
     4441 atctcctcgg cggcattttt gcccgcgtgg aagccgctgg gcatcgaacg gcccagcata
     4501 tcggtgccaa cgttggaggt catgatccag atagcgtgct gcgcatcgac ggtatgcccc
     4561 tgcgcatcgg tcaggcggcc agcgtcgaag acctgcaaga acaggtcaaa gacctctgga
     4621 tgcgccttct cgacctcgtc gagcaatacc acgcagtgcg ggcggcggcg caatttgccg
     4681 gtgagctgac cctcctggtc atggccgatg tagccgggag gcgcgccgac catgcgcgag
     4741 acggcgtgtt tctccatgta ttccgacata tccacgcgga tcaggtgttc atcggagccg
     4801 aagacctcgg ctgccagggc gcgcgccagc tcggttttgc cgacgccggt cgggcctaag
     4861 aagagaaaga cgcccgatgg gcgatggcgt tgcttcagtc ccgcgcgcga tacctggatg
     4921 gcctgcgtga cgcgcgcgat ggcggcatcc tggccgatca cgcgccgctt gagcagcact
     4981 tcgaggttga gcagccggtc gcgttcctcg cggcctggcg cgcgcaccgg gatgccggtg
     5041 cgacgcgaga ttacctcggc gatcatcggg ggtataatca gcgggggatc gataaagaca
     5101 tcatccagtt catcctcatt ggcgttctcg ttctcttcag ccaggtcgac gcgcgcctgc
     5161 gagcaggctt catccagcac actgcacgct ttatcgggca ggcgcaggtt cggcaggtac
     5221 tgcacggaga gatgcaccgc cgcgtagagc gtatctttgg cgatttcgac tcggtgatgc
     5281 tgctcgtaga gatcgcgcat cccggtcaga atagccagcg cctcttcctc ggatggctcc
     5341 tcgatctgga tggggcgcag gcggcgctcc atcgcggcat ccttttcgat ggtgcgatac
     5401 tcctgcggcg tggtggcgcc gatgcagcgc agccgcccgc ccgccagcgc gggtttgaga
     5461 ataccggcgg cgtcgatact gccatcggcg gcgcgccccg tgccgatcaa caggtgcatc
     5521 tcgtcgatga agaggataat gtcgccgctg ccctcggcct cctccagcac tttgagcagc
     5581 cgttcctcga attcgccccg gtacttggtt cccgccacga gcgagctggc ggagagttcg
     5641 atcaggcgtt tgccgcgcag ttccagcggc accttgccat cgacgatgcg ctgcgccaga
     5701 ccggtgacga tggtcgtctt gccgacaccg gagtcgccga tcaggatggg gttgttgcgg
     5761 tctttcagca tcagcgtctg gatcagcagg cgcatctcgt tctcgcgccc gatcagcggc
     5821 ggcagttgct tcatgctggc ctgtttggtc aggtcgcgtc ccaggcgttc gagcgtagag
     5881 ttgccgcttt tcatgccccc ttcggggccg atgcctaccg gcaattcgat gggcgcggcg
     5941 gcaggattgg cgctgacgga cgggacgagt tccaggatgg cgcgagcgtt gctgttcatg
     6001 accgtatcga tcaggtgcga tgggttaatg cccattttag tcagcaactc gtaggtaacg
     6061 ccatcgcgct cgctcagtac tgcctgggcg atagcgcgtt catcgatcga actggagcca
     6121 gctttcaggg cgttgccttc ggcggtctgg agaatttctt tacaacggcg cgtcggcaga
     6181 ataggcgtat cgctggtggc tttgccgttg cccagcgcga tgcgtatcac gtcgcgtact
     6241 tgcttgggcg aaaagttgag gttgcgcaga gcatcctggg tgcagccgcc atccagtttg
     6301 gtgagcgcaa tgaacagatg cggtgtgccg agatagtctt tgtgcatccg ccgcgcctcc
     6361 gtcagggcga tgcccagcac ttcctggagg aagcgccggt ccagcgcgtt ggatgagagg
     6421 acagagggca gcaggtgatc gtcttccagc gagtcaagcg gcagctccag gttgcggtcg
     6481 cgcccacctg cgggggccgt cacaagcgta ctggtcgggt tgccatacag cacatagcac
     6541 agccaggatg gatcatcagg atggcgctgc gccatcgtgg agcgcgcgcg ccgtagggcc
     6601 tcgccctgcg agatgccgtc ggccagttcc tggtagagca gcacggcaaa ttcacgcgcg
     6661 cgtggggacg cgaccggcca gcgcatcgcc agcaccgcgc ccgctcccgc cgccagcaaa
     6721 ttcgtgatcg ccagcgcttc gatttcgtag ggcggcagcg tgccaacctg ccccccgtcg
     6781 tagtagctca acatcacgag cggatgcctg ggcaatgcc
//
LOCUS       DASZAK010000773         2198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_481166, whole genome shotgun sequence.
ACCESSION   DASZAK010000773 DASZAK010000000
VERSION     DASZAK010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481166"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VGT44_13605"
     CDS             complement(<1..364)
                     /locus_tag="VGT44_13605"
                     /inference="COORDINATES: protein motif:HMM:NF025205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HEV2581884.1"
                     /translation="MPTNAVTPYAPVVLKLLQNPLFSDESTAWNLLLTHITPVQEYFA
                     RIGLEVRIHEDDGFAYLRQPALEDEEGQSIALPRLTRRDRLTYHTTLLCIILREWLDQ
                     FESTHLDAGKCVINDDQLR"
     gene            complement(354..1835)
                     /locus_tag="VGT44_13610"
     CDS             complement(354..1835)
                     /locus_tag="VGT44_13610"
                     /inference="COORDINATES: protein motif:HMM:NF023283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
                     /protein_id="HEV2581885.1"
                     /translation="MTVMDYDQLTYDLQNAPGIRLLKADHAALIISFLHRQFKREQRI
                     AVPLPELAERLADVLEALNEQAPGSFPRPAHSYLTEWADERHRFVRITTPASSDLPLV
                     ELTADAERAIGWLEELHGQAFVSTESRFLYIVQLLREIVQKSTEDPTARLAQLEQQRD
                     ALQQQIDQIRETGIVDDLYTTTQLKERFFEACNGARQLLRDFRLVEERFRGMARALQE
                     AQLQSNAQKGALVAYVLDADAELKSSDQGRSFYTFWDFLMAPSQQDELYRLLEAVQQQ
                     ADLQPVIHEGKILRHLPMHLLGAGEKVVQSNAGLAQQLRRMLDEQNIAERRRVRAMVS
                     EIKQLAFRATGLPPDEAAFIELEGPPQVHLVMEHALWEPGESFSVSAQPTSTPDILPE
                     RDELLSLHTQFFIDRAMLARRIEALLEIRSQITLAEVLECYPPEKGLAEVLAYCALAA
                     DDPQHRIDEQQTETIELNAARREQGMRTVTIPRVLYQRRDYAN"
BASE COUNT          397 a          652 c          694 g          455 t
ORIGIN      
        1 ctcgcagctg gtcgtcgttg atgacgcatt tcccggcatc aaggtgcgtg gactcgaact
       61 ggtcgagcca ttcgcgcagg atgatgcaga gcagcgtggt gtggtacgtg aggcggtcgc
      121 ggcgcgtcag gcggggcagc gcaatcgatt gcccctcctc gtcttcgagg gcgggctggc
      181 gcaggtaggc gaaaccgtcg tcttcgtgga tgcgcacttc gaggccgatg cgcgcgaagt
      241 actcctgcac cggcgtgatg tgcgtcaaga gcaggttcca ggccgtcgac tcatcgctga
      301 acagcgggtt ctggagtagc ttcagcacga cgggcgcgta tggcgtgacg gcgttagttg
      361 gcataatccc tcctctggta taacacgcgc gggatggtga cggttctcat tccctgctcg
      421 cgcctggcgg cgttcaactc gatcgtctcg gtctgctgtt cgtcgatacg atgctgcggg
      481 tcgtctgcgg ccagcgcgca atacgccagc acctcggcca gacccttctc cggtggatag
      541 cattccagca cctcggccag cgtaatctgg gagcgaatct ccagcagcgc ttcaatacgg
      601 cgggcgagca tagctctatc gataaaaaac tgtgtgtgca gcgaaagtag ctcgtctctc
      661 tccggcagga tatcgggagt gctggtcggc tgcgcgctga cggagaacga ttcgccgggt
      721 tcccatagcg cgtgttccat caccaggtga acttgcggcg gtccttccag ttcgatgaag
      781 gcggcttcat cgggagggag acctgtggcg cggaaggcca gttgcttgat ttcggagacc
      841 atggcgcgca cgcggcggcg ttcggcgatg ttctgctcgt cgagcatcct gcgcagttgc
      901 tgggccagtc ccgcgttcga ttgcaccacc ttctcgcccg cgcccagcag gtgcatgggc
      961 aggtgccgca gtatcttgcc ttcgtggatg acgggttgca ggtcggcctg ctgctgcacg
     1021 gcctcgagca gccggtacaa ttcgtcctgc tgcgagggcg ccatcaagaa gtcccagaag
     1081 gtgtagaagc tgcgtccctg gtcggatgat ttcaattcgg cgtcggcatc cagcacatag
     1141 gccaccagcg cgcccttctg cgcgttggac tgcaactgcg cctcttgcaa ggcgcgcgcc
     1201 atgccgcgaa agcgttcctc gaccagccgg aaatcacgca gtagctgccg cgccccgtta
     1261 caggcttcaa agaaacgctc ttttagctga gtggtagtgt acaggtcatc gacgatgccc
     1321 gtctcgcgaa tctggtcgat ctgctgttgc agggcgtcgc gctgctgttc gagctgtgcc
     1381 aggcgcgcgg tcgggtcctc ggtactcttc tgcacaatct cgcgcagcag ttgcacgata
     1441 tagaggaagc gcgactcggt gctgacgaac gcctggccgt gcagttcttc gagccagccg
     1501 atggcgcgtt ccgcgtccgc cgtcagctcg accagcggca aatcgctgct ggcgggcgtg
     1561 gtgatgcgca caaagcggtg gcgctcgtcc gcccactccg tcaggtagga atgagccggg
     1621 cgcggaaaac tgcctggcgc ctgctcgttc aacgcctcaa gcacatccgc gagccgctcg
     1681 gccagttccg gcagtggcac agcgatgcgc tgctcgcgct tgaactggcg atgcaggaag
     1741 ctgataatca acgcggcatg gtcggccttg agcagcctga tgcccggcgc gttctgcaaa
     1801 tcataggtaa gctggtcgta atccatcacg gtcattggga aatatccttc ccgctcttgc
     1861 tctctccagt gcgttcttcc acaagtctgt gcgcttatta tattggttct tcgcgaggag
     1921 tgaaaatccg gtaatcattt gggggatata atatatatgg aatcatcgca aaccccggta
     1981 ggtgccggtt taccgcgcac atcgccgatt tatcggccag tgtcgaggga atacagcaag
     2041 cacccaggcc gataatgcag attgacaaaa tacctcggct accggaagag gacgagggcg
     2101 accgcaaggg tctccacccc gctccacgcc acccccgccc ttacaaagaa cggcggattg
     2161 gcgtgtcaag cggcttcgta gtcattgtaa gggcgggg
//
LOCUS       DASZAK010000774        11341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_54304, whole genome shotgun sequence.
ACCESSION   DASZAK010000774 DASZAK010000000
VERSION     DASZAK010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54304"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VGT44_13615"
     CDS             complement(<1..129)
                     /locus_tag="VGT44_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581886.1"
                     /translation="MNTAARAILHLDLDAFYASIEQRDHAEYRGKPVIVGGSPDRRG"
     gene            complement(203..679)
                     /locus_tag="VGT44_13620"
     CDS             complement(203..679)
                     /locus_tag="VGT44_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV2581887.1"
                     /translation="MKALSVMQPWATLIALGAKCVETRSWSTSYRGPLAIHASGRMSR
                     EAAMSLRVPDIREALAAGGYHQGSGLVSNLCGLPLGAVIAVVTLVDVQRITPDNIPAE
                     PERSFGDYTPGRFAWFLHDVRRLPEPFEAKGALGLWEWKANQGPIEFSGASHQLSD"
     gene            complement(694..1842)
                     /locus_tag="VGT44_13625"
     CDS             complement(694..1842)
                     /locus_tag="VGT44_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV2581888.1"
                     /translation="MAEQAQFQRARARALGISIGTLSQGPYNAITDVAGVRVGHATLV
                     RGSGPLVRGVGPVRTGVTALLPHGDNLFTSKVSAAATVFNGFGKSIGLVQIAELGQLE
                     TPILLTNTLNIGKVADALVACMVEQNPAIGVTTGTVNPVVCECNDGWLNDIGGRHLGE
                     QEVRAALNNAAGGSVLEGNVGAGTGMITYGFAGGIGTASRRLAVEQGGYTLGALLLAN
                     FGRRDELLIAGVPVGQELEGRRQPLPTTTEPPREKREQGSVIVLLATDAPLDARQLGR
                     LARRVPLGLARTGTHGGHGSGDLAIAFSTAQRLPHDSTPLAHTMTVLNEQHPAIDALF
                     AAVVETTEEAVLNSLFAAHSIDGRDNHRVDALPVDEAIAILQQWRSGR"
     gene            2092..3066
                     /locus_tag="VGT44_13630"
     CDS             2092..3066
                     /locus_tag="VGT44_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2581889.1"
                     /translation="MDYRRFGRTGLKVSCFCLGTMQWGWTADESTAFAVMDAFVERGG
                     NFLDTADFYSRWLPGHVGGESEEIIGRWMQQRGNRQQMIVATKVYQPMGEHPNDRGLS
                     RKHIIEALEASLRRLRTDYIDLYLAHAFDAETPIEETLEALDDARRAGKIRYAGASNY
                     PAWRLVEALWKAEVHNTPRFDCLEPHYNLVHRAEFERDLETICQQYQIAVLPYSPLAN
                     GFLTGKYRRGQVQDTESARAGSVRQRYDREEAWQALAAVEQIAQESSSTPAAIALAWL
                     LAQPVVTAPILGARSISQLEACLEALTVTLSPDHLARLDAASTWKTSG"
     gene            complement(3076..4209)
                     /locus_tag="VGT44_13635"
     CDS             complement(3076..4209)
                     /locus_tag="VGT44_13635"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEV2581890.1"
                     /translation="MSSERTTGKSAAALNGICPYFTMFPLAFPSTVLRTRAAPGDWVC
                     DPFCGRGTTNYASRMLGLPSIGIDSNPVAVALSQAKLANTNPIHIMQVAHRILDEVAE
                     PGDVPQGEFWEWAFYKDVLHTLCRFREGLLKNCRSDARKALRAIMLGALHGPRQKQRA
                     SYFSNQSQRTYAPKPRYAVSYWKARNLIPQPVDVLDVIGVRAQRYYANETTKAVGRII
                     AGDSRDGEVYDRLTTQRAIRWVITSPPYYGLNTYLPDQWLRSWFLGGPAQVDYSTQGQ
                     ISHAGPEIFASQLGEVWQHVGGECAAGAKMVIRFGAINDRKVDALELLKQSLYGSGWE
                     ITDVQSAGSASSGHRQALHFSPSRKSAIEEYDVWGVWRGANLV"
     gene            complement(4202..6343)
                     /locus_tag="VGT44_13640"
     CDS             complement(4202..6343)
                     /locus_tag="VGT44_13640"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEV2581891.1"
                     /translation="MPDWELFPRNQIVGIFRGFQEGGLEFHADLVLPYRNIFQNIPMH
                     GQFLLVQLETPDEAVLGRIASFSAEGKLASGYGEEFNIRALRENRVVPDDLREDYLKY
                     RVNIRVLGVLRKIGDDLKFVPSHRRLPHVGSPVAFPSGEVLRFIAGHDDAGAVIGHFA
                     LGEYIYAEGSKYGQVQEWMQVKSPEILVRFKVDHLVARRSFIFARAGFGKSNLNKLLF
                     STLYSETPTTPRHVGKDAPVGTIIFDPEGEYFWPDHDDRPGLCDVPALQDKLVVFTSR
                     KGPSGFYDSFVAGDIKLDIRRLSPSDVLGIALGPDRQEQQNVRKLKGLDQRSWEKLVN
                     LIDQFGNQAPLEEIGKILGLEANKQEIEALAARGNMTAIVRTLHDKSSQFMDMLIKAL
                     SLGKLCVVDLSRMRGSQSLILSGLILRRIFDRNQEEFTKAEPKTIPTIAVVEEAQTVL
                     NERATSSEPYIAWVKEGRKYDLGALLITQQPGSIPVDILSQGDNWFIFHLLSSTDLTT
                     LQRANAHFSADILSVLLNEPIKGQGVFWSSVGERAYPISVRVLSFEQLYPRLDADYTR
                     TEVYTFARQLKAEFKQAVEERLEAISGPLADVTVDSSDGTLPEEEAEPIDVMSVYKQQ
                     VIASLSHKREFLERMRTQKGLAWGVVKGYIVEELPQAIDERERGDIAYNLVREVLVAV
                     FGPEDEGWHTFTASRKDGRPTMWVKAGKNNE"
     gene            complement(6367..7512)
                     /locus_tag="VGT44_13645"
     CDS             complement(6367..7512)
                     /locus_tag="VGT44_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581892.1"
                     /translation="MIEPESYYQLKREIAERIEAERAVLDQLRAEIRPLRSAVRRIQP
                     RTTTSISLVATDGGNNKLQFDPFLVQVVRIVDSSNNEYCLEAVTPSTKIASLSARQFN
                     VEGEPLTALGLLMDYLQVRNLEQLSHMIRHQEDGTPTSPTWVQVYRDLVEWAILFSLV
                     RTGNYSTDTMIIRDGLLRSKIFAGDLFARYLDGLQEGIEQQWRQKRRRIYLTGVAKHS
                     QVLSRYRLAMMLERILTTTYAAYVEIPREIEEKAYLWSEYARGSDREITGREGNKFVG
                     GKMFFVKFGSGPRDPIWPIDIYLPQVDVAQHILGCMLADAQNGFPIPHYPLCLQKAHA
                     NAALVDFDFDVLQNNIFDGIRAILGDEAPVLDVFRLQESDPAQRRYE"
     gene            complement(7634..9511)
                     /locus_tag="VGT44_13650"
     CDS             complement(7634..9511)
                     /locus_tag="VGT44_13650"
                     /inference="COORDINATES: protein motif:HMM:NF038324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HEV2581893.1"
                     /translation="MTITRKRHHVGELRPSQVLYTYGIGSVVDLPFISAMVMGLDDWE
                     HPQPVTIHEERLLQSVRALLGQQVTRLLAPPEAREPAPGSYNPFDESTLVGVPVAAFP
                     RWMLCPRCRTLASLDSGLFQLKADVYRPDRTRFVHANCSSAGSPPAVVPARFLVACKN
                     GHLDDFPWLTFVHGGVPCQARLKLRELGVTGEAADIQVLCETCGKQKRMSEAFGIEGR
                     QNLPPCSGRHPHLRSYDKARCQEQSRAILLGASNSWFPLLLSTLAVPSAGDPLSQLVE
                     EHWRLLSNTLNQQNIALLRSIGELEQFAAYADEAVWQEVEAQRRAAAAGESEGQGAKP
                     VSLKAPEWGVLTHPQTAPVARDFKVTAVAPPDGFEQAIKQVALVERLREVRALVGFTR
                     IEAPGDFSEMDEVPEARRAPLSRRPPTWIPAAEVRGEGIFLEFREEALSAWLERRELR
                     LYERSFYEAHVQWRTARRVAEPALGYPGARYVLLHSLAHALMRQLTLECGYTAASIRE
                     RIYALPPEHDDGPMAGILLYTAAPDSEGTLGGLVGLGTPTLLGHHLDGTLERMASCTS
                     DPLCAEHHCVQDHSLHEAACHACLFLPETSCERGNKYLDRSVLVPTVDRAHLAFFSGG
                     M"
     gene            complement(9508..>11341)
                     /locus_tag="VGT44_13655"
     CDS             complement(9508..>11341)
                     /locus_tag="VGT44_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEV2581894.1"
                     /translation="IRQDHGHYQRGSTIGGIGSPRQLTSCPWCGEPIDAGRDITVEPP
                     ERGRGRTFIYCGDALGRCPFSRRNAPEGLPVLVVDEEIYRCLPALLIATVDKFAQMPW
                     NGATQMLFGQVSKRCTRHGFRCPDLDDQDQHRAIGNLPRAHSVPHAPLRPPDLLIQDE
                     LHLISGPLGTLVALYESVVDRLCSWEVDGHVVRPKVIAATATVRQASEQVHALFLRRV
                     NVFPPQGLDARDNFFSRQRDPCEAAPGRRYLGVCAQGRRLKAALIRVYVAYLSAAQSL
                     FQQYGQAVDPWMTMVGYFNSMNELGGMRRLVEDDVRNRLGRMQQRGLANRYPPILEEL
                     TSRRSSTDIPEVLEKLARVFDPARDKDAPRPVDVALATNMISVGVDVQRLGLMVVTGQ
                     PKTTAEYIQATSRVGRAAPGLICVVFNWARPRDLSHYEQFEHYHATFYQQVEALSVTP
                     FAPRALDRGLAALLVSYVRLLGAEFNENSRAEHVSEARFYIQAALRELPRRAVAVTGS
                     AAAGAEVRAALEELEAYWTMKTRATSGTRLGYKTRRDGQTIGLLRQPGQGEWLPFTCL
                     NSLRDVEPAINLILDERGVGETLEQAASVPVESEQEDEQGRQQA"
BASE COUNT         2133 a         3543 c         3458 g         2207 t
ORIGIN      
        1 gccgcgccgg tcgggggagc cacccacgat gaccggcttg ccccgatatt cggcgtggtc
       61 gcgctgctcg atgctcgcgt aaaaggcgtc taggtcgaga tgtaagatgg ctcgcgctgc
      121 agtgttcatg cttttggctg ctcctgggtc cagcggtctc tccttcttgc tcgtgttttc
      181 attgtaccag cttcagcact ggtcagtcag acaattggtg tgacgctccg ctgaactcga
      241 tcggtccctg gttcgccttc cattcccata gcccgagcgc gccctttgcc tcgaaaggct
      301 caggcagccg tcgcacatcg tgaaggaacc aggcaaagcg tccgggcgtg taatccccga
      361 atgaccgctc cggttctgct gggatattgt ccggcgtgat acgctgcaca tctaccagtg
      421 tgacgactgc gattactgcg ccaagtggca acccgcatag attgctcacc aggccacttc
      481 cctgatgata tcccccagct gccagcgcct cccgaatatc tggtacgcgc aggctcatgg
      541 ctgcctctct actcatcctg ccagaggcgt ggatcgccag tggaccacga taggaagtcg
      601 accatgagcg cgtttccaca cacttggctc cgagggcgat cagcgtcgcc cagggctgca
      661 tgacgctcaa tgctttcatc ggcagaacac ctttcacctg ccgcttctcc actgctggag
      721 gatagcgatg gcctcgtcaa ctggcagggc atcgacgcga tgattatcgc ggccatcgat
      781 gctgtgggca gcgaagagag agttgagcac ggcttcctcc gtcgtttcca ccaccgctgc
      841 gaacagggca tcgatggccg ggtgttgttc attgaggaca gtcatcgtgt gggcgagtgg
      901 agtggaatcg tgcgggagac gctgggctgt ggaaaaggcg atggccaggt cgccactgcc
      961 atgaccgcca tgagttcccg tgcgcgccag cccgaggggc actcggcgcg ccaatcgccc
     1021 caactggcgc gcgtcgagtg gagcgtcagt ggccagcagc acgatcaccg atccctgctc
     1081 gcgtttttcc ctcggtggtt ccgtcgtggt cggcaggggt tgcctccgcc cctccaactc
     1141 ctgcccaact gggacgccag cgatgagcaa ttcgtcccga cgaccgaagt tggccagcag
     1201 cagcgccccc agcgtgtaac ctccctgctc tactgcgagc cgcctggaag cggtcccgat
     1261 gccacccgcg aagccgtagg tgatcatgcc ggttcctgcg ccgacgttgc cttcgagcac
     1321 cgacccgcca gcagcgttat tgagcgcagc gcgtacttcc tgctccccga ggtgacgacc
     1381 gccgatatcg ttgagccagc cgtcgttgca ctcgcagacg accgggttga ccgtcccggt
     1441 ggtgacgccg atggccggat tctgctccac catgcaggcg accagcgcgt cggcgacctt
     1501 gccaatgttc agagtattgg tcagcaggat gggcgtctcg agctggccga gttcggcgat
     1561 ctgcaccagg ccgatcgatt tgccgaagcc attgaagaca gtggccgccg ccgagacctt
     1621 cgaggtaaag agattgtccc catgggggag cagcgccgtt accccggtgc gcaccggccc
     1681 aacaccgcgc actagaggac ccgatcctcg cacgagggta gcgtgtccca cgcggacacc
     1741 ggcaacatcg gtaatggcgt tgtacggccc ttgggagagc gttccgatgc tgatgcccaa
     1801 cgcacgtgct cgtgcgcgtt gaaactgcgc ttgttccgcc atggcagctt gctcctttct
     1861 ggattctcgg aagtcgcgcg tattttgagt atacgccgtg atcaactaca tggaaagact
     1921 tgcagatcaa gtgctcactg ctttgctcgc cgaccgctcc ttaagccacc gagcagacaa
     1981 gactctcgag agcggtgtat tgattacgtt gattacgacg agtatagtga tgatggtaat
     2041 cgtttctcta ccgttcttaa atatcactgg cgaaaggcgt gaccagttca tatggactac
     2101 cgcagatttg gccgcaccgg tttgaaggtc tcctgctttt gtctcggcac gatgcagtgg
     2161 ggctggactg ccgacgagtc gacggcattc gccgtaatgg atgcgttcgt ggagcgcggc
     2221 ggcaatttcc tggacacggc ggatttctac tcgcgctggc tcccagggca tgttggagga
     2281 gagtcggaag agatcatcgg gcgctggatg cagcagcgtg gcaaccgcca gcagatgatc
     2341 gttgccacca aagtctatca accgatgggg gagcacccca acgatcgggg gctctcgcgt
     2401 aaacatatca tcgaagcgct ggaagcttcc ctgcgccgcc tgcgcaccga ttatattgat
     2461 ctgtacctgg cgcacgcctt cgatgccgag actcccatcg aggagacgct ggaagccctc
     2521 gatgacgcgc gccgcgccgg caagatccgc tacgcgggtg cctccaacta tccggcctgg
     2581 cgcctggtcg aggcgttgtg gaaggccgag gtgcataaca ccccgcgttt tgactgtctg
     2641 gagccccact ataacctggt ccaccgcgcc gagttcgaac gcgacctcga gaccatttgc
     2701 cagcaatacc agatcgccgt gctgccctac agcccgctgg ccaatggctt cttaacgggc
     2761 aaataccggc gtgggcaggt gcaggacact gagagcgcaa gggcaggatc ggtgcggcaa
     2821 cgctacgacc gggaagaggc atggcaagcc ctggcagcag ttgagcagat cgcccaggaa
     2881 agcagcagca cccctgccgc tatcgcgctc gcctggctgc tcgctcagcc ggtggtaacc
     2941 gcacccattc ttggcgcccg ttccatctca caactcgaag cctgcctgga agcactcacg
     3001 gtgactctct cgcctgacca cttggctcgc ctggacgcag cctcgacctg gaaaacgagc
     3061 ggatgatctg ggggttcata cgaggttagc tcctctccat accccccaca cgtcgtattc
     3121 ctcaatcgcg cttttcctgg aaggcgagaa gtgcagcgcc tgcctgtgtc cgcttgaggc
     3181 ggaccctgcc gactgcacat cggtaatctc ccagccggaa ccatacaatg actgcttcag
     3241 cagttcaagc gcgtccactt tcctatcatt aatcgccccg aagcgaatca ccatctttgc
     3301 ccctgctgca cactcgccac caacgtgctg ccagacctcg cccagttggg aagcaaagat
     3361 ctccggccca gcatggctga tctgcccttg cgtcgagtag tccacctgtg caggcccacc
     3421 taaaaaccag gagcgcagcc actgatcggg tagatacgta ttcaagccgt agtacggcgg
     3481 agacgttatc acccatcgaa tcgctcgttg cgtggtcagt cgatcataca cctcgccatc
     3541 ccggctgtct ccggcaatga tacgcccgac cgctttcgtc gtttcgttcg cataatagcg
     3601 ctgcgcacgg accccaatga cgtccaggac atcgacgggt tgcggtatga gattgcgtgc
     3661 cttccagtag ctgaccgcgt agcgcggttt gggcgcatag gtccgctgtg actggttcga
     3721 gaaataggat gcccgctgtt tctggcgcgg gccgtgcagc gcccccagca taatggcccg
     3781 cagcgctttc cgcgcatccg agcggcaatt cttcagcagc ccctcgcgga aacggcagag
     3841 tgtgtgcagc acgtccttgt aaaaggccca ctcccagaac tcgccctgtg gcacatcacc
     3901 tggctcggcc acctcgtcga gaatgcggtg tgctacctgc atgatgtgta tcggattcgt
     3961 attcgccagc tttgcctgcg agagcgccac cgccaccggg ttgctatcga tgccgatgga
     4021 gggcaaaccc agcatgcggc tggcgtagtt cgtggtcccc ctcccacaaa acggatcaca
     4081 gacccagtct cccggtgccg cacgggtgcg taacactgta gaggggaacg caagcgggaa
     4141 catggtgaaa tatgggcaga tgccgttgag ggcggctgcc gactttccag tcgtacgctc
     4201 gctactcatt gtttttcccc gcttttaccc acatggtcgg cctcccatcc tttcttgagg
     4261 cggtaaaggt atgccagccc tcgtcctccg gcccaaatac cgcaacaagg acttcccgaa
     4321 cgaggttgta ggcgatatcc ccgcgttccc gttcatcgat cgcttgcggc agttcctcaa
     4381 caatataccc tttgacgacc ccccaggcca gccctttctg ggtgcgcatc ctctccaaga
     4441 attctcgctt gtggctcaag gaagcaataa cttgctgctt atagaccgac atcacgtcga
     4501 tcggctcggc ctcttcctct gggagggtgc catcactcga atcgaccgtc acgtcggcga
     4561 gcggaccgct gatcgcctcc aatcgctctt caacggcctg tttgaattcg gccttgagtt
     4621 gccgggcgaa cgtgtagact tcggtgcggg tgtagtcagc atccaggcgg ggatagagct
     4681 gttcaaacga gagtacgcgt acagagatgg gataggctcg ctcccccacg gaactccaga
     4741 agacgccctg ccctttaatc ggctcgttca agaggacgct gagaatgtct gcactgaagt
     4801 gcgcgttggc ccgctgcagg gtcgtcaggt cggtggaaga aagcaggtgg aagatgaacc
     4861 agttgtcacc ctggctgagg atatcgaccg gaatgcttcc gggctgctgc gtaatgagca
     4921 aggcgccgag gtcgtatttg cgaccctcct tgacccaggc gatatacggc tcggaggagg
     4981 ttgctcgctc gttgagcacc gtttgtgcct cttctacgac ggcaatggtc gggatcgttt
     5041 tcggttcggc tttggtaaac tcttcctggt tgcggtcaaa gatacggcgc aagatcagac
     5101 cggagagaat aagcgactgg gaaccacgca tccgtgaaag atcgacgacg cataacttgc
     5161 ccagtgatag cgccttgatg agcatatcca tgaactggct gcttttatca tgcagcgtcc
     5221 gaacaatagc ggtcatgttc ccgcgagcag caagtgcctc gatctcttgc ttattcgctt
     5281 cgagtccgag aattttcccg atttcctcga ggggtgcctg gttgccaaac tgatcgatca
     5341 ggttcaccag cttttcccaa ctgcgctgat ccagcccttt gagcttgcga acgttttgct
     5401 gctcctggcg atcaggtccg agggcgatac caagcacatc cgaggggctc agcctccgga
     5461 tgtcgagctt gatatcgccg gcgacaaagg agtcatagaa tccgctggga cctttccgcg
     5521 aggtgaagac gaccagctta tcctggagcg cggggacatc gcacagccct ggtcgatcat
     5581 catggtcggg ccagaagtac tcaccttcgg ggtcaaagat gatcgtaccc accggtgcat
     5641 ccttgccaac atgtctgggg gttgtcggcg tttcgctgta cagagtgctg aaaaggagct
     5701 tattgagatt tgatttgcca aaccccgccc gcgcgaagat gaaggaacgc ctggccacca
     5761 ggtgatccac cttgaaacgc accaggattt caggggactt tacctgcatc cactcctgca
     5821 cctgtccata cttgctcccc tccgcataga tgtattcgcc cagcgcgaag tgcccaatca
     5881 cagcccctgc atcatcgtgc cctgctataa agcgcaggac ttccccagaa gggaaagcca
     5941 ctgggctgcc aacgtgcgga aggcgtcgat gtgaagggac aaacttcagg tcgtcgccga
     6001 ttttcctcag cacaccgagc acccgaatat tcacccggta cttgagataa tcttcgcgca
     6061 aatcatcggg aactacgcgg ttttcacgca gcgcccggat attaaattcc tcgccgtatc
     6121 cagaggcaag tttgccctcg gcggagaagg aggcgatgcg tcccaggaca gcctcgtcgg
     6181 gcgtctcaag ctgaaccagg agaaattgcc cgtgcatcgg aatgttctgg aagatgttcc
     6241 ggtaaggcag gacaaggtcc gcgtggaatt ccaagccacc ctcctggaag ccgcgaaaga
     6301 tgccgacgat ctggttgcgg ggaaacagtt cccagtcagg catacgctcc ctccttttgt
     6361 tgctctttac tcataacgac gctgcgcggg atcggactcc tgcaggcgaa acacatccag
     6421 cacgggagct tcatccccca aaatcgcgcg gattccatca aagatgttgt tctggagcac
     6481 gtcgaagtcg aaatcgacga gcgcggcgtt ggcgtgggcc ttttgcaggc agagtgggta
     6541 gtgggggatg gggaagccgt tctgcgcatc agccagcata cagcccagga tatgctgtgc
     6601 cacatctact tgcggcaggt agatatcgat cggccagatg gggtcgcgag ggccactgcc
     6661 aaatttgacg aagaacatct tcccacccac gaatttgttg ccttcccgtc cagtgatttc
     6721 tcggtcactg ccgcgcgcgt actccgacca gaggtaggcc ttttcctcga tctcgcgcgg
     6781 tatttcgaca tacgccgcat aggtcgtggt gaggatgcgt tccagcatca tggcgaggcg
     6841 gtagcgcgaa agcacctggc tgtgtttggc gacaccggtc aggtagatgc ggcggcgctt
     6901 ctgccgccac tgctgctcaa tcccctcttg taaaccatcc aggtagcgcg caaacagatc
     6961 ccccgcaaaa attttgctgc ggagcagacc atcgcggatg atcatggtat cagtgctgta
     7021 attacctgtt cggaccaacg agaagaggat ggcccactca accaggtctc gatatacctg
     7081 cacccaggta ggactggtag gggtgccatc ctcctggtga cggatcatgt ggctcagttg
     7141 ctccaagttc cgtacctgca aatagtccat caggaggccg agagccgtga gcggctcgcc
     7201 ctcaacattg aactgcctgg cgctgagact ggcgatcttc gtgctgggag tcacggcctc
     7261 gaggcagtac tcattattac tcgaatcaac aatgcgcacc acttgaacca gaaaggggtc
     7321 aaattgcaat ttgttgtttc cgccatcggt ggcaacgagc gagatggatg tggtcgttct
     7381 tggctgaatg cggcgaaccg cgctacgcag cggtcggatc tcggcacgca gttgatccag
     7441 cacggcacgc tcggcttcga tgcgctcggc tatttcccgc ttgagttgat aatagctctc
     7501 cggttcaatc attagatgag agtagttgcc cccgatttca aataatctgc tcagatgtaa
     7561 gatagtggaa attctaccag tatcttagca ctaccgtaag tacgatgcaa tagatgtatg
     7621 aacgtagtca cgctcacatg cctccgctaa agaacgccag gtgcgcccga tcgacggtcg
     7681 gcaccagcac tgagcggtcg aggtacttgt tgccgcgttc gcaggaggtc tccggcagga
     7741 agaggcaggc gtggcaagcc gcttcgtgca gcgagtgatc ctggacgcag tgatgttcgg
     7801 cacagagcgg gtcggaggtg cagcttgcca tgcgctccag cgtgccgtcg aggtgatggc
     7861 caagcagggt gggcgtcccc aggccgacca gtccacccag cgtgccctcg ctatcgggcg
     7921 cggcggtgta gagcaggatg cccgccatcg ggccatcgtc gtgttcgggc gggagcgcgt
     7981 agatgcgctc gcggatgctg gccgccgtgt agccgcactc cagcgtgagc tggcgcatca
     8041 gggcatgggc gagcgagtgc agcaggacgt agcgcgcgcc gggataaccc agggctggct
     8101 ccgccacgcg ccgcgctgtg cgccactgca catgcgcctc gtagaaggaa cgctcataca
     8161 gacgcagctc gcgtcgctca agccaggccg agagcgcctc ctcgcgaaat tccaggaaaa
     8221 tcccctcgcc gcgcacctcg gcggcgggta tccaggtggg aggccggcgc gacagcggtg
     8281 cgcgccgcgc ttcagggacc tcgtccatct cgctaaaatc gcctggtgct tcgatgcgcg
     8341 taaagccgac cagcgcgcgc acctcgcgca gacgctccac caacgccacc tgcttgatgg
     8401 cctgctcgaa gccatcaggc ggcgccaccg ccgtcacctt gaaatcgcgc gcgacaggcg
     8461 cagtctgtgg gtgcgtcagc acaccccact ccggcgcctt caggctcaca ggtttcgcgc
     8521 cctggccctc gctctcaccc gcagcggcgg cgcggcgttg tgcctcgacc tcctgccaga
     8581 cggcttcgtc tgcgtaggcg gcgaattgct ccagttcccc gatgctgcgc aggagcgcga
     8641 tattctgctg gttcagcgtg ttcgagagca gccgccagtg ttcctccacc agttgcgaga
     8701 gtggatcgcc cgcactgggg acggccagtg tggagagcag caagggaaac cagctattgg
     8761 aggctcccag caggatggca cgcgactgct cctggcacct cgccttgtcg tagctgcgca
     8821 ggtgcgggtg gcgcccactg cagggcggca gattctgccg gccctcaatg ccgaaggctt
     8881 cggacatgcg cttctgcttg ccgcacgtct cgcacagcac ctggatgtcc gccgcctcgc
     8941 cggtgacgcc gagttcgcgc agcttcagcc gcgcctggca gggaacgccg ccatgcacga
     9001 aggtgagcca ggggaaatcg tccaggtgcc cgttcttgca ggccaccagg aagcgcgcgg
     9061 gtaccactgc cggcggtgag cctgccgatg agcaattggc atgcacgaag cgtgtgcggt
     9121 ccgggcggta gacatcggcc ttcaactgga acagacccga atcgagcgag gccagcgtgc
     9181 ggcagcgcgg gcagagcatc cagcgcggga aggccgccac cggcacgccc accagggtgc
     9241 tctcatcgaa ggggttgtag ctgcccggcg caggctcgcg ggcctccggc ggcgcgagca
     9301 ggcgcgtgac ctgctgcccg agcagcgcgc gcaccgattg cagcagtcgc tcttcgtgga
     9361 tggtgacggg ctggggatgc tcccaatcat cgagtcccat caccatggcc gagatgaagg
     9421 gcagatcgac aaccgagccg atgccgtagg tgtagagcac ctggctgggt cgcagctccc
     9481 cgacgtggtg gcgttttctt gtgatagtca tgcctgctgc ctcccttgct cgtcttcctg
     9541 ttcgctttcg actggcacac tggctgcctg ttcgagcgtc tcgccgacgc cgcgttcgtc
     9601 cagaatcaga ttgatggcgg gttccacgtc gcgcagcgag ttgaggcaag tgaagggtag
     9661 ccactcgccc tggccgggct gccgcagcag gccgatagtc tgcccatcgc ggcgcgtctt
     9721 gtagccgagc cgcgtgccgc tcgtggcgcg cgtcttcatt gtccagtacg cttccagctc
     9781 ttccagtgca gctcgcacct cggcgccagc agcggcgctg cccgtcacag ccacggcgcg
     9841 gcgcggcaac tcgcgcagcg cggcctgtat gtagaagcgc gcctcgctga cgtgctcggc
     9901 ccggctgttc tcgttgaatt ccgctcccag caggcgcacg tagctgacca gcagcgcggc
     9961 caacccccgg tcgagcgcgc gtggggcgaa gggcgtcacc gaaagcgcct cgacctgctg
    10021 atagaaagtg gcgtggtaat gctcgaactg ctcgtagtgg gagagatcgc gcgggcgtgc
    10081 ccagttgaag acgacgcaga tcaggccggg cgcagcgcgc ccgacgcggc tggtggcctg
    10141 gatgtattct gccgtcgtct tgggctggcc ggtcacgacc atcagtccca ggcgctgcac
    10201 atccacgccg accgagatca tgttggtggc cagcgccacg tcgaccggcc tgggcgcatc
    10261 cttgtcgcgg gcggggtcga agacgcgcgc cagcttctcc agcacctcag gaatatcggt
    10321 cgagcttcga cgcgaggtca gttcctcgag gatcggcggg taacggttgg ccagcccgcg
    10381 ctgctgcatc ctgcccaggc gattgcgcac gtcgtcctcg accaggcggc gcatcccacc
    10441 cagctcgttc atggagttga agtagccgac catcgtcatc caggggtcaa cggcctgccc
    10501 gtactgctgg aagagagact gggcggcgga aagataagcg acgtagacgc ggatcagcgc
    10561 ggccttgagg cgccggccct gggcgcagac gccgagatag cgccgtcccg gcgcggcctc
    10621 gcaggggtcg cgctggcggg agaaaaagtt gtcgcgtgcg tccagcccct gcggcggaaa
    10681 tacgttgacg cgccgcagga agagcgcgtg cacctgctcg ctggcctgcc gcacggtagc
    10741 ggtcgcggcg atcacctttg gccgcacaac atgtccatcg acctcccagg agcacaggcg
    10801 atcgacgacc gattcgtaga gcgccaccag cgtcccgagc gggccggaga tgaggtgcag
    10861 ctcgtcctgg atcagcaggt caggcgggcg cagcggcgcg tgcggtacgc tatgcgccct
    10921 gggcaggttc ccgatggcgc ggtgctggtc ctggtcgtcc aggtcggggc agcggaagcc
    10981 gtggcgcgtg cagcgtttgc taacctgccc gaagagcatc tgcgtcgcgc cgttccaggg
    11041 catctgcgcg aacttatcga cggtggcgat cagcagcgca ggcaggcagc ggtagatttc
    11101 ctcatccacc accagcaccg gcagcccctc cggcgcgttg cgccggctga aggggcagcg
    11161 ccccagcgca tcgccgcagt agatgaaggt gcgaccccgc ccgcgctccg gcggctcgac
    11221 ggtgatgtcg cgcccggcat caatgggctc cccacaccag ggacaactcg tcaactgacg
    11281 cggggaaccg atgccgccga tggtgctgcc gcgctggtag tggccgtgat cctgccggat
    11341 g
//
LOCUS       DASZAK010000775         1565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_694771, whole genome shotgun sequence.
ACCESSION   DASZAK010000775 DASZAK010000000
VERSION     DASZAK010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694771"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1565
                     /gene="drmD"
                     /locus_tag="VGT44_13660"
     CDS             <1..>1565
                     /gene="drmD"
                     /locus_tag="VGT44_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DISARM system SNF2-like helicase DrmD"
                     /protein_id="HEV2581895.1"
                     /translation="ALLELLDNQRFARGVRVNREQLHAVMVRRLKSELPPRWDGTPRF
                     PQRVLEPLEVSYTPEEREVHRLLQAYTQSRLAQTGDETEQYATEFVLKLLKKRLFSSP
                     EAFAITLRQHEESLANARRRPTGAFKTTVGILRRQIEQTEEEGDSDEALEEARAAALD
                     AAAPLFREPGADERRLLAQMRTWATTARTRPDSKAATLIEWLQRTLKPTGAWSTRRVI
                     LFTEYRATQKWLLDLLERTGLAEPGRLLTLYGGMRPEERERIKAAFQADPRESDVRIL
                     LATDAASEGIDLQNYCHRLVHYEIPWNPNRLEQRNGRIDRHGQRSPEVNIYHFVSSKF
                     RSAALDTALNVGELDDDLEFLMRAVRKVESIREDLGKVGPVIAEQVEEAMLGHRRALE
                     TRSAEDKTAPIRSLLKLERRLKEQIDRLHEQLRDSRQELHLTPEHVQAAVETALELAG
                     QPPLHHVFLRDPNGERPTIDAFSVPALRGSWAMCKEGLEHPFTHQERPIVFDQEAVQE
                     RDDVVLAHLNHRL"
BASE COUNT          329 a          510 c          481 g          245 t
ORIGIN      
        1 cggccctgct ggagctgctc gataaccagc gcttcgccag gggcgtgcgc gtcaatcgcg
       61 aacaactgca tgcagtgatg gtgcgccgtc tcaaatcaga gctcccgccg cgctgggatg
      121 gcacgccgcg tttccctcag cgcgtgctgg aaccgctcga agtctcctat acgccggaag
      181 aacgcgaggt ccaccgcctg ctgcaagcct acacccaatc gcgcctggcg cagacgggcg
      241 atgagacgga acaatacgcc accgagtttg tactcaaact gctcaagaaa cgcctcttct
      301 cctcgcccga agcattcgcc attacgctgc ggcaacacga agaatcgctg gcgaacgccc
      361 ggcgccgtcc caccggcgct ttcaagacca cggtgggcat cctgcggcgg cagatcgagc
      421 aaactgaaga ggagggtgat agcgacgagg cgttagaaga ggcgcgcgct gccgccctgg
      481 acgccgcggc gccgcttttc cgtgagccag gcgccgacga gagacgcctg ctcgcacaga
      541 tgcgcacctg ggcgacgacc gcacgcaccc ggcccgatag caaggcagcc accctgatcg
      601 agtggctgca acggacactc aagcctaccg gcgcgtggtc aacacggcgc gttattctct
      661 ttaccgagta tcgcgctacg cagaagtggt tgctcgacct gctggaacgc actggtctgg
      721 ccgagcctgg ccgcctgctc acgctctacg gcggcatgcg accggaggag cgcgagcgga
      781 tcaaagcggc gttccaggcc gaccccaggg aatcggatgt acgcatcctg ctggcaaccg
      841 acgccgcctc cgagggtatc gacctgcaaa actattgcca tcgtcttgtc cactacgaga
      901 taccctggaa ccccaaccgc ctcgaacagc gcaacgggcg catcgaccgg cacgggcagc
      961 gttcgccgga ggtaaatatc taccacttcg tgagtagtaa gtttcgcagc gcggcgctgg
     1021 acacggcgct caacgtcggc gaactcgatg acgacctgga atttttgatg cgcgccgtgc
     1081 gcaaagtcga atccattcgc gaggacctgg gcaaagtcgg cccggtgatc gcggaacagg
     1141 tggaagaggc tatgctggga caccgccggg cgctggaaac ccgctcagcc gaggataaga
     1201 ccgcgccgat acgcagcctg ctgaagcttg aacgccggct caaagagcaa atcgacagac
     1261 tgcacgagca attaagggac agccgacagg agttgcacct gacgcctgaa catgtccagg
     1321 cggcggtgga gaccgcgctg gaactggccg ggcagccccc gttgcaccat gtcttcctgc
     1381 gcgacccgaa cggagaacga cctaccatcg acgctttctc cgtccctgcc ctgcggggaa
     1441 gctgggcgat gtgtaaagag gggctggaac atccctttac ccaccaggaa cgtcccattg
     1501 tcttcgacca ggaggcggta caggaacgcg atgatgtcgt gctggcgcat ctgaaccacc
     1561 ggctc
//
LOCUS       DASZAK010000776         6437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_694773, whole genome shotgun sequence.
ACCESSION   DASZAK010000776 DASZAK010000000
VERSION     DASZAK010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_694773"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..1381
                     /locus_tag="VGT44_13665"
     CDS             8..1381
                     /locus_tag="VGT44_13665"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV2581896.1"
                     /translation="MTKKAVDNYISQHEARFLEDLKDWLRIPSISTLPEHAGDIRRAA
                     EFAAGQLRGIGFEGVQVIATKGHPLVYGEWLKAPGKPTVLVYGHYDVQPVDPIELWES
                     PPFEPTIRGDNLYSRGACDDKGQTMLVFKALESLMAVNGKLPVNVRVLIEGEEEAGGA
                     SIEDYVKTYPERLACDAAFICDTHMPSKELPALITGLRGIVYTEVEVRGARRDLHSGS
                     YGGVAPNPMHALAIIIAGLKDAQGHIHISGLYDNIRPAPDVEKTFWYQDPLHMNEMLL
                     EEMGVSEFTGEAEYPPLERIWNRPTLEVHGIAGGFTGEGAKTVIPAVGKAKISLRLPA
                     NLKPDEAFALFERRVKELTPRGVEVFVHKVHGGDAVIVPIDAPPMQAAAAALKETYGK
                     DPIFIREGGSIPVAALFYNLLNVPVVMMGFGLPDDNLHAPNEKYSLSQYFKGIRTVTR
                     FLSKLGE"
     gene            complement(1498..2211)
                     /locus_tag="VGT44_13670"
     CDS             complement(1498..2211)
                     /locus_tag="VGT44_13670"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEV2581897.1"
                     /translation="MVKHITPHKARHLPGESTYERYLRVPELLNLQKPEQELAHSDEL
                     QFQVVHQVFELWWKESAFELRRIRSLLSEANLPLALKLMRRVIATQHLMLANMRMLET
                     MSPWDFHAFRQVLDDGAGTDSPGFHALMSLSPLLWDDFAALLDREQVTLADIYTRADR
                     YPLLLAFAEALTDYDEVFQLFRSQHFKLARRMIGPGSIGTGGTPMDILERTLKDVFYP
                     ELWEIRNQLTKIADEQGLK"
     gene            complement(2289..2783)
                     /locus_tag="VGT44_13675"
     CDS             complement(2289..2783)
                     /locus_tag="VGT44_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581898.1"
                     /translation="MTIALVILSSCGAATGQAATPSPTQTPAPIIVPSPSPTLVPTPS
                     PTPSPTPKPAVTPQPTTPPAAPAILDVRPQSMSIVGHLDCTKNGSYMCQALVLSRSSA
                     ASNLHWFAYTNVPGHIVFSPASGTLAPGQSTLITISVPFNACTTGLFFFQGPINTHTI
                     TWAC"
     gene            complement(3028..3627)
                     /locus_tag="VGT44_13680"
     CDS             complement(3028..3627)
                     /locus_tag="VGT44_13680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2581899.1"
                     /translation="MQEFQSFYEDNIDLIYRYVYSRIRNRQEAEDLTSSIFVKAMRGI
                     DLARDAKSRRSWLFQTAHTTIADYWRAYYRGETASLDALLETGWETPDETAYAHVGSQ
                     AAECVQDILQALPERYRNVLVSRFLLNLSVRETAAKMGMTEANVKITQYRALRFAANL
                     ENAPPAQCHPAQCHPAHCHPERSEGSAPGSARDPSLRSG"
     gene            complement(3813..5468)
                     /gene="guaA"
                     /locus_tag="VGT44_13685"
     CDS             complement(3813..5468)
                     /gene="guaA"
                     /locus_tag="VGT44_13685"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV2581900.1"
                     /translation="MTHERRQAIIVLDFGSQYSRLITRRVRELRVYSELVPAHTTLAQ
                     LRAQPHLDIRGFILSGGPSSVYDVDAPLCDPAILASGLPVLGICYGMQLLAHQLGGHV
                     APASGRREYGPATLETLPDAALDERCYRIFEGINATPAQTNPLDQPRQSHATTPLRIP
                     VWMSHGDSVDALPGGFSVLARTEGNPITAIANAQGMVGLQFHPEVTHTPQGRDILRNF
                     VYRICGCDPSWTTSSVIDEAVATIRQRVGGQRVLCGLSGGVDSAVAALLIHRAVGEQL
                     TCVFVDTGCLRAGERDQVVETFSDHMHIPLVVVDAQRRFLGRLADVVDPEAKRKIIGE
                     EFIRVFEEEAARLQADGSPLRFLAQGTLYPDVIESTSHDTAATATRIKTHHNVGGLPQ
                     DIAFELVEPLRMLFKDEVREIGGALGLPQEWVWRHPFPGPGLAIRVIGAVDEQKLETL
                     RAADAIVIDEIRRAGVYRELGQAFAVLTPLQSVGVMGDGRTYANVVAVRAVTTGDFMT
                     ADWARLSPDLLARISHRIVNEVPGVNRVVYDITSKPPATIEWE"
     gene            complement(5475..6236)
                     /locus_tag="VGT44_13690"
     CDS             complement(5475..6236)
                     /locus_tag="VGT44_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HEV2581901.1"
                     /translation="MGRLDGRVAFVTGAGRGIGAATALRLAEEGARVALADLDVAGCE
                     QVAAEINKLGSEAIALECNVADAAMVQNAVDSAANHFGRLDILVNNAGVTRDNLLFKM
                     SDDDWETVMNVHLRGAFLCSRAVQKYMVQNKFGRIVSLSSTSALGNRGQANYSAAKAG
                     LQGFTRTLAIELGQFGITANAIAPGFIDTEMTRATARRQGLDPDEVIAQASKTIPVRR
                     VGQPRDIANVICFLCSDEASFVSGQVIYVAGGPRG"
BASE COUNT         1301 a         1825 c         2061 g         1250 t
ORIGIN      
        1 ctaaaacatg acaaaaaaag cagtggacaa ctatatttca cagcatgaag cgcggttctt
       61 ggaggatttg aaggattggc tgcgcattcc cagcatcagc acgctgccgg aacacgcggg
      121 cgatatccgg cgcgcggcgg agttcgcggc agggcagctg cgcggcattg ggtttgaggg
      181 ggtacaggtt atcgcgacga agggacaccc gctcgtgtat ggcgagtggc tcaaagcgcc
      241 gggcaagccg accgtgctgg tgtatgggca ttacgatgtc cagccggtcg atcctatcga
      301 gttgtgggag tcgccgccgt tcgagccgac gatacgcggc gataacctct atagccgggg
      361 agcgtgtgac gacaagggac agaccatgct ggtcttcaag gcgctggagt cgctgatggc
      421 ggtcaacggc aaactgcccg tcaatgtgcg cgtgttgatc gagggcgaag aagaggcggg
      481 gggcgcgtcg attgaagatt acgtcaagac ctatcccgaa cggttagcgt gtgacgcggc
      541 cttcatttgc gacacccaca tgccgtcgaa ggaactgccc gcgctgatta cgggactgcg
      601 cggcatcgtt tatactgagg tcgaggtgcg cggtgcgcga cgcgacctgc actctggctc
      661 atacggcggc gtcgcgccta acccgatgca cgcgctggcc atcatcatcg ccggcttgaa
      721 ggacgcgcag ggccacattc atatctccgg actctatgac aacattcgcc cggcgcctga
      781 tgtcgaaaag accttctggt atcaagaccc gctgcacatg aatgagatgc tgctggaaga
      841 gatgggtgtc agcgagttta ccggcgaggc cgaatatccg ccgctggagc gcatctggaa
      901 tcgcccgaca ttggaggtgc atggcatcgc cggaggcttt accggcgagg gcgcgaagac
      961 agtcattccc gccgtcggca aggccaagat cagcctgcgc ctgcccgcca acctgaagcc
     1021 cgacgaagcg tttgcgctgt tcgagcggcg cgtcaaggaa ctgacgccca ggggcgtcga
     1081 ggttttcgtt cacaaagtgc atggcggcga cgcagtgatt gtgccgatcg atgccccgcc
     1141 catgcaggcg gcggcggcgg ctctcaagga gacctacggc aaagacccaa tcttcattcg
     1201 cgaaggcggc tctatccctg tcgcggcgct gttctacaac ctgctgaacg tgccggtcgt
     1261 gatgatgggc ttcggcctgc cggacgacaa cctgcatgca cccaacgaga agtattcgct
     1321 ctcgcaatac ttcaagggca tccgcaccgt cacgcgcttc ctgtcgaagc tgggtgaata
     1381 aacaaacgac atggctgcgt tgagcatggc gacggcaagc gtcgccacta cgataacgtg
     1441 ggaaacgcca catttccgtt atcgtagtgg cgacgctgcc gtcgccatgc ggttcgttta
     1501 tttcaagccc tgttcgtcgg cgattttggt caactggttg cgtatttccc agagttcggg
     1561 gtagaagaca tctttgaggg tgcgttccag gatatccatg ggggtgccgc cggtgccgat
     1621 ggagccgggg ccgatcatgc gccgcgccag cttgaaatgc tgcgagcgga agagctggaa
     1681 gacctcatcg taatcggtca acgcctcggc gaaggccagc agcaagggat agcggtcggc
     1741 gcgcgtgtag atgtcggcca gcgttacctg ttcgcggtcg agcagggcgg caaaatcatc
     1801 ccacaggaga ggcgagagcg acatgagggc gtgaaagccg ggcgaatcgg ttccagcgcc
     1861 atcatcgagt acctggcgaa aggcgtgaaa atcccagggg ctcatcgttt cgagcatgcg
     1921 catattggcg agcataaggt gctgtgtggc gatgacacgc cgcatcagct tgagcgccag
     1981 cggcagattc gcttcgctca gcagcgagcg gattctgcgc aattcaaagg cggactcttt
     2041 ccaccatagc tcaaagacct ggtgaacgac ctggaactgc aattcatcgg aatgcgctaa
     2101 ctcttgctcc ggcttctgta aattcaatag ttctggaacg cgcaggtagc gttcataggt
     2161 gctttcccct ggtaaatgcc tggctttatg gggcgtgata tgtttgacca tagcggctct
     2221 cctccttctc tggaatgtga tggaaacaac gctgtttcag tatcagaata tcacattcca
     2281 tccagcgatt agcaggccca ggtgatggtg tgcgtgttga tcggtccctg gaagaagaac
     2341 aggccggttg tgcaggcgtt gaacggcaca gagatcgtga tgagcgtact ctggccggga
     2401 gcaagcgtgc cactggcggg ggagaagacg atatgccccg gcacattcgt gtaggcgaac
     2461 cagtgcagat tgcttgcagc gctcgacctg gacagcacaa gcgcctggca catatacgac
     2521 ccgtttttcg tgcagtcgag atgcccgacg atgctcatcg actgtggtcg cacatcgaga
     2581 atagctggcg cggctggagg cgtggtcggc tgcggtgtga cggcaggttt tggcgtcggc
     2641 gagggcgtcg gtgatggtgt tggtacaagg gtcggcgatg gagacggaac gattatgggc
     2701 gcgggcgttt gcgtgggaga tggcgtcgcg gcctggcctg ttgcagcgcc gcaggaagac
     2761 agaataacga gagcgatggt cataacgaca acaacaataa aactacactt ttgtattttt
     2821 ttagacgatt ggagtgaatg ggcaaccgac ccgcgaaaac cgagatggaa cataataatc
     2881 ctttctgaca tacatttgag caagatgcgc aatatctaaa gtatgttcag tcggaggtta
     2941 catctagcag gctagcgggg tcgtctaatc gttcaggcac ggcaaccgca agggaccgcc
     3001 atcataggcg ttcacgcccg cccactgtca tcctgagcgc agcgaaggat ctctcgctga
     3061 gccgggcgca gatccttcgc tgcgctcagg atgacagtgg gcaggatgac actgggcggg
     3121 atgacactgg gcgggtgggg cgttttcgag atttgcggca aacctgaggg cgcggtactg
     3181 cgtgattttg acgttcgcct cggtcatgcc cattttcgcg gcggtttcgc gtaccgagag
     3241 attgaggagg aagcggctga ccagcacatt acgatagcgt tccggcagcg cctggagaat
     3301 gtcctgcaca cactctgctg cctggctgcc aacatgagcg taggcagttt cgtccggggt
     3361 ttcccagcca gtttccaaca gggcatccag cgaggcagtt tcgccgcgat aataggcgcg
     3421 ccagtagtcg gcgatggtgg tgtgggcagt ctgaaacagc cagctccgcc tgctcttcgc
     3481 gtcgcgcgcc aggtcgatgc cgcgcatagc cttgacaaag atgctcgatg tcaggtcctc
     3541 ggcctcctgg cgattccgaa tcctgctata cacatagcga tagatcaaat cgatattgtc
     3601 ttcataaaaa ctctggaatt cctgcatagt tgctcccaga ctactgacgc aaggacgaac
     3661 ccgcgtgatc gccttcggga cccattccct gtgtctggag caagtgtagc tttacagagg
     3721 catctttgac atcgtgagaa acgtctattt cgcgtaacct tgttgcgtag cacgaagcgg
     3781 gagatattgg cagaattacc tatgtgcctt tatcattccc attcaatcgt ggcgggcggt
     3841 ttggaggtga tatcgtagac gacgcggttg acgccgggaa cctcgttgac gatgcggtgg
     3901 gagatgcgcg ccagcaggtc gggggagaga cgcgcccagt cggccgtcat gaagtcgccg
     3961 gtggtgacgg ctcgcactgc caccacgttg gcgtaggtgc ggccatcgcc catcacgccg
     4021 acgctttgca ggggggtcag gacggcaaag gcctggccaa gctcgcgata gacgccggcg
     4081 cggcggattt cgtcgatgac gatggcatcg gcggcgcgca gcgtctccag cttttgctcg
     4141 tctaccgcgc cgatgacgcg gatggccagg ccgggaccgg ggaagggatg ccgccagacc
     4201 cactcctgcg gcaggccgag cgcgccgccg atctcacgca cctcgtcctt gaagagcatt
     4261 cgcagcggtt cgaccagctc gaaggcaata tcctgtggca ggccgccgac gttgtgatgc
     4321 gtcttgatgc gcgtggcggt cgcggcggta tcgtggctgg tgctttcgat cacatcgggg
     4381 tagagcgtcc cctgcgcgag aaagcgtagc gggctgccat cggcctgcaa gcgcgccgcc
     4441 tcttcctcaa agacacgaat gaactcctcg ccgatgatct tgcgcttggc ttcaggatcg
     4501 accacgtcag caagcctgcc caggaaacgc cgctgcgcat ccacaacgac cagggggata
     4561 tgcatatgat cgctgaaggt ctccaccacc tgatcgcgct cgcccgcgcg caggcagccg
     4621 gtatcgacga agacgcaggt gagctgttcg cccacagcgc gatggatcag cagcgccgcg
     4681 accgccgaat cgacgccgcc ggatagcccg cacaacacgc gctggccccc gacgcgctgt
     4741 cggattgtcg cgaccgcctc atcgatgacc gaactggtcg tccaactcgg gtcgcagccg
     4801 cagatgcggt aaacgaagtt gcgcaggata tccctgccct gcggcgtatg cgtcacttcg
     4861 gggtgaaatt gcagcccgac catgccctgc gcattggcga tggcggtgat cggattgccc
     4921 tcggtgcgcg ccagcacact gaaaccgccg ggcagcgcgt caacgctgtc gccgtgactc
     4981 atccagacgg ggatgcgcag gggagtggtc gcgtgtgatt gacgcggctg atcgaggggg
     5041 ttggtctgcg caggtgtcgc gttgatgccc tcgaagatgc gataacatcg ctcatccagc
     5101 gccgcgtcgg gcagggtttc gagcgtggcg gggccgtact cgcggcgacc actggcgggc
     5161 gcgacatgcc cgccaagttg atgcgccagc aactgcattc catagcagat gcccaggacg
     5221 ggcaggccgc tcgctaagat ggccggatca cagagcgggg cgtccacgtc atagacactc
     5281 gacggaccgc cggagaggat gaagccgcga atgtcgaggt gtggctgcgc ccgcaactgc
     5341 gccagcgtgg tatgcgcggg aaccagctcg ctgtagacgc gcaattcgcg cacacgcctg
     5401 gtgatgaggc ggctgtattg cgagccgaaa tccagcacga tgatggcctg ccgtcgttcg
     5461 tgggtcatag tagtttatcc gcgtgggccg ccggcgacgt agatgacctg gccgctgacg
     5521 aaactggctt catccgagca gaggaagcaa atgacgttgg cgatgtcgcg cggctgcccg
     5581 acgcggcgca cgggaatggt tttggatgcc tgcgcgatga cctcgtcagg gtcgagaccc
     5641 tggcggcggg cagtggcgcg ggtcatctcg gtatcgatga agccgggcgc gatggcgttg
     5701 gcggttatgc cgaattgtcc cagctcgatc gccagcgtgc gcgtgaagcc ctgcaagccg
     5761 gccttggcgg cggaatagtt ggcctggccg cgattgccca gggcggatgt cgaggaaagc
     5821 gagacgatac gcccaaactt attctgtacc atgtacttct gcacggcgcg gctgcacagg
     5881 aacgcgccgc gcagatgcac attcatgacg gtctcccaat catcgtcgct cattttgaag
     5941 agaaggttat cgcgcgtcac tccggcgtta ttgacgagga tatccagccg cccgaaatgg
     6001 ttggcggcgc tatcgacggc gttctgtacc atagccgcgt cggcgacgtt gcattccagc
     6061 gcgatggcct ccgaaccgag cttattgatc tcggcagcga cctgctcgca acccgccaca
     6121 tcaaggtcgg cgagggccac acgcgcgcct tcctcggcca gccgcagcgc cgtcgcagcg
     6181 ccgatgccgc gacccgcgcc cgtcacaaaa gcgacgcgcc catctagtcg tcccatgcgt
     6241 tatgctcctt ccacttctcc gaccggctga gcgcagcgca ctccttgcat cttgcctaag
     6301 cgaagctagg acgcagtctg ttggcaagat ggctttcaag aagaacgcag ttcgttgccg
     6361 gtcggaataa gatcacctta cgaaagaact aaatttgtta gagtatatca caatggatag
     6421 tctaacgagg cgatagg
//
LOCUS       DASZAK010000777         7358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_481202, whole genome shotgun sequence.
ACCESSION   DASZAK010000777 DASZAK010000000
VERSION     DASZAK010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481202"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..523
                     /locus_tag="VGT44_13695"
     CDS             260..523
                     /locus_tag="VGT44_13695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581902.1"
                     /translation="MQSNNRLGTIDLHYTTSNVHGTLILERELDETDLTKLIEQIDED
                     LVLSADMPREDFLVSVYVGRDVGFYSDDYFTEENSLSDEDEDI"
     gene            complement(527..1177)
                     /locus_tag="VGT44_13700"
     CDS             complement(527..1177)
                     /locus_tag="VGT44_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2581903.1"
                     /translation="MTIHILLVDDHTILRAGLKMMLNAQPDMEVIGEAQDGRHAIMEA
                     TRLRPDVVLMDITMPDLNGIEATRQIKKQLADVKVLVLTMHENEEYVFQALRAGASGY
                     ILKEAADTELITALHVVQNGNFYLSPTAQSVMVGDYLQRVRSGEEKDSYSSLTERERE
                     ILKLVAEGYTNNQIAERLIISPKTVDTHRTHVMDKLNLHSRAELVKYAMRRGLLED"
     gene            complement(1174..2271)
                     /locus_tag="VGT44_13705"
     CDS             complement(1174..2271)
                     /locus_tag="VGT44_13705"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2581904.1"
                     /translation="MGTSHIQPTGESQAGNRLLRRVLSIPIFYKVLFANSLIIFVGAT
                     GGTWLATHLNTSPYATPWSLVIFVALGWLISIALNFVVLQIAFRPLTHLGKVMQRVEA
                     GERELRAPLTGIDIQADRLASAFNLVLEALDEAARQRASQIINAQEQERQRIARELHD
                     ETSQVLTSLLISLAVLEESIESPEARERIADTRSLAHSTLRAIRNLSIDLRPSALDDL
                     GLLPALRWYVKEYQKKCSIEVEFHSSGLKERLPAEMETALYRIVQESLTNTARHANAS
                     KVRISLKETGDAIKAAIIDNGQGFDYEALLKNPGEGRGLGVAGMQERAVLLNGTLQID
                     SEPGKGTKVVVYIPLPTGEQTPLRKTDEVTV"
     gene            2692..3498
                     /gene="extP"
                     /locus_tag="VGT44_13710"
     CDS             2692..3498
                     /gene="extP"
                     /locus_tag="VGT44_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HEV2581905.1"
                     /translation="MSTLPNKSLLDTITQKMVESQVWRSIFRHGYPDTPLNQSLVMMS
                     NVFLHLHPVKISRQAMKVTYTWCMGGISFFLFLLLTITGVFLMFFYVPDTTLAYQNIN
                     TLSTAVSFGHLVRNMHRWAAHLMIVSVTCHMIRVFYHGAYKPPREFNWVIGVLLFFFT
                     LFLSFTGYLLPWDQIAIWAITVGTNLAPYTPLLGDAVYKVIVGGSAVSQLTLVRFYVG
                     HVILFPLVLSLLLAVHFWRIRKDGGVAGPPPPSRREVEARVEPTAVGSAR"
     gene            3563..4105
                     /locus_tag="VGT44_13715"
     CDS             3563..4105
                     /locus_tag="VGT44_13715"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581906.1"
                     /translation="MSIEQQEPKAQTQAGATQRRYRTLAVVKQEAITRVERPLEDSVF
                     VWPHLLVREFFASAIVMVMITVLSIVIDAPLREPANPNVTPNPAKAPWYFLGLQELLH
                     YFSPNVAGVLVPGLTLTALIVLPYVDRNPSRAYADRKVAIVTFTMFLVFWAFITIAGS
                     FFRGPGWIWVWPWQHMYFDL"
     gene            4132..4818
                     /locus_tag="VGT44_13720"
     CDS             4132..4818
                     /locus_tag="VGT44_13720"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEV2581907.1"
                     /translation="MSLDTPPISPQQYEVLHGGGAEAAEIAQQRLSRRRFLRRSMLAV
                     WGLSATAAVAGGLYMTYPNLAGQFGSDLTIGTKSQFPAAKPQDYKINQAGIFYVQEAK
                     TYLSHIAAGTPTLLNGSSLSDQLDSESFVKDKDGSLWVALYQRCVHLGCTVPFRDDCV
                     SFKCPCHGSHYNVDGEYLDGPAPRSLDRFSMSFSGDSVVVSTGTLNNKVPHPDSTTRI
                     IAVPGVQCSV"
     gene            4877..5905
                     /locus_tag="VGT44_13725"
     CDS             4877..5905
                     /locus_tag="VGT44_13725"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEV2581908.1"
                     /translation="MISPVQAAVGIIVALLAVGALLYTFYSRTNSVEKTGYGALMMLS
                     IISLMIPIFWIVESNGETISQLQQHNTAVNRGASLFAQYCFQCHGIFGQGVVGPKLNG
                     NTAVNNLSDSDILRIISGGIYDPANPTTALMPAWSDQFGGPLTEDQIQYLFQLIRSAD
                     PAYLSKNGFPTGPGTNGFDQVPGLVQQSQPIPYSTAIAQATAGSGIGQFPFVDLTSRP
                     SIIIQIIDSPTGAACNPACYAVADPKNASQLIIDPNIKVKVGTKITWINKSKTIHTVT
                     SLVGEVPTVQVPAKVFDSGLTTPMAAGTGVYSYTVATQAYTFNSNHIVVYYCQFHPSM
                     VAAITVTQ"
     gene            6021..>7358
                     /locus_tag="VGT44_13730"
     CDS             6021..>7358
                     /locus_tag="VGT44_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581909.1"
                     /translation="MQQTQQREQGDRISWARAMIFGVGFFFIAALLVGQFPSYVYYQM
                     TSSSLTAIEGGFLALGAICLAGFVVIQVIVFLFDPKPVIPPIVFSVLGLGLSVVGVAI
                     MLWAVVTGNQGFPNASTNIAPVLNGKFLWFEPGDIDIVMLGLVIMGVGVAMLFFSALA
                     YQEQRNPDRRDLGTTPAIRALIWVGLTMLAAFLLFYTFFSDTQLAAQIYPQCPIAINA
                     AKGCPGPITGLFIVRTIFNTFLGIAIFCTLGAFALRLHYLMRPVRKRTMSSLYMIGIN
                     LAQFGAIFLVAWLVLYPLIAWMHSWTFIGLGDYLTLCAKKSAIPQSCAFSAQAGYILA
                     AVVCGGSFTLLMAAIAVWKTRRNLVVISGVTLTAVLSLATLVVHTNPDEVLIAALLCG
                     GGLGLAAIWTSVARREFAVVGENNLGCVGQWLVLGTCLLIYIAAFAFFSLPVFV"
BASE COUNT         1463 a         2057 c         1994 g         1844 t
ORIGIN      
        1 aatgggcacc tacggtttcg ctggtgcagt taatattacg ttgtaacgtc tatgcgaata
       61 gcgagaagaa tgatataata acggcgtctt aaccgacagt atgctctttt aagtctcttc
      121 caggcgtcga tgagaaagga gggcgcagcg cactaattac aagggctgcg cgcagaacgc
      181 acctatggat gatattcgat ttgagcggca tttccgcaca ccatactctg aaagctacta
      241 tattttgcaa ggaaccagca tgcaaagcaa taaccggctt ggcacgatcg acctgcacta
      301 taccacctcc aatgtgcatg ggacgctgat cctggagcgc gagctggatg aaaccgatct
      361 gacgaaattg atcgaacaga tcgatgaaga cctggtgctg tctgccgata tgccccgcga
      421 ggatttcctg gtcagcgtct atgtaggcag ggatgtaggg ttttatagcg atgattattt
      481 caccgaggaa aacagtctca gcgacgagga cgaggatatc taaacgctaa tcttccagta
      541 agccccggcg catagcgtat tttaccaact cggcgcgact atgcaggttg agcttgtcca
      601 tgacgtgggt tcgatgtgta tccaccgttt tcgggctgat gatgagccgc tccgcaatct
      661 ggttattggt gtagccttct gcgactagtt tcaggatttc gcgctcgcgt tcggtcaggc
      721 tgctataact atctttttct tctccagagc ggacgcgctg caggtaatcc cccaccatca
      781 ccgattgcgc ggtgggagag agatagaagt tgccattttg cactacatgc agcgcggtga
      841 ttagttcggt gtccgccgcc tctttgagga tgtagcctga tgcgccagcc cgcaatgcct
      901 gaaacacgta ttcctcattt tcatgcatag tcagcaccag caccttcacg tctgccagtt
      961 gcttcttgat ctggcgcgtg gcctcgatgc cgtttaaatc gggcatggta atgtccatga
     1021 ggaccacgtc gggacgcaga cgtgtagctt ccatgatggc atgacgcccg tcctgcgcct
     1081 cgccgattac ctccatatcg ggctgcgcat tcagcatcat tttcaagccc gcgcgcagaa
     1141 tggtgtgatc atctactaaa agaatgtgta tagtcatacg gtcacttcat cagtctttcg
     1201 taatggtgtt tgttcgccgg tgggtagagg tatgtagacg accacttttg tgcctttccc
     1261 aggctctgag tcgatttgca gtgtgccatt gagcaatact gcgcgttctt gcatgcctgc
     1321 cacgcccagc ccgcgaccct caccggggtt ctttagtagc gcctcgtaat caaagccctg
     1381 gccattgtca ataatcgctg ctttgatggc atcgccggtc tcttttaagg agatgcgcac
     1441 tttgcttgcg ttcgcgtggc gagcggtgtt cgtcaaactt tcctgcacta tccgatacag
     1501 cgccgtctcc atttcggcag gcaggcgctc tttcagaccg ctcgagtgaa actccacctc
     1561 gatggagcac ttcttctggt attctttgac gtaccagcgt agcgcaggca gcaagcccag
     1621 gtcgtccagg gcgcttggcc gtaaatcgat gctcagattg cggatcgcgc gcagggtgga
     1681 gtgagccagc gagcgtgtgt cagcaatgcg ttcacgcgcc tccggcgact cgattgattc
     1741 ctctagaact gccaggctga taagcagtga tgtcagcacc tggctggttt catcgtgcag
     1801 ctcgcgggcg atgcgctgcc gctcctgctc ctgggcgttg atgatctggc tggcgcgctg
     1861 gcgcgctgct tcgtccagcg cctccagaac caggttgaag gcgctggcga gccggtctgc
     1921 ctgtatgtcg atgcccgtca gcggtgcgcg cagttcgcgt tcgcctgctt cgacgcgctg
     1981 catgactttg cccaggtgag tcaggggccg gaaggcaatc tggagtacga cgaaattgag
     2041 cgcgatactg atgagccagc ccagcgccac gaaaatcacc aatgaccagg gtgttgcata
     2101 gggactggtg ttgagatggg ttgcaagcca ggtgccgcct gtcgccccca caaaaatgat
     2161 gaggctattt gcgaacagca ccttatagaa aatggggatg ctcaggacgc gacgtaagag
     2221 gcgatttcct gcctgacttt ccccagtcgg ttgtatatgg gatgttccca ttgtgtcgat
     2281 cctttcaata tccatgcata ttgtaggatg agctgagtgg cctgtcaaat cgcaggcgaa
     2341 tcaggttccc tgtgcgcaaa tatcaggtgt gtacctgatt ggaggggttg ttcaatgctt
     2401 gtatagtaaa gagtgaatac tcattctgcg catacctcgc tatcgcctta caaatacgat
     2461 acatcgaaga ggaaactctt ctcaattgtc gtaggatgcg ttacaattac taatagcagg
     2521 agctgtgact cttgttgtgt ggtcatggct tacacaacag gtgtgtacgg cgtggctttg
     2581 agtatgatat aatttgccac gcgcaaccag gacttttcaa atacctgaac attgtcttta
     2641 caaaaggcaa tctcaaaccg agatgtttca agacttagga aggcaggcac ggtgagtact
     2701 cttccaaata aaagcctcct cgacacaatc acgcagaaga tggtcgagag tcaagtttgg
     2761 cgctcaatat ttcgccatgg ctatcctgat accccgttaa accagtcgct ggtgatgatg
     2821 agcaatgtat ttctgcattt gcatcccgtg aaaatcagcc gccaggctat gaaagtcacc
     2881 tatacctggt gcatgggtgg tatttcgttc tttctctttc tgctgctgac catcacgggc
     2941 gtcttcctga tgttcttcta tgtgcccgat accactctag cctaccagaa catcaacacg
     3001 ctcagcaccg ccgtgtcatt tgggcacctg gtgcgcaaca tgcatcgctg ggctgcccac
     3061 ttgatgatcg tctcggtgac gtgccatatg attcgcgtct tctatcatgg cgcgtacaag
     3121 ccgccgagag agttcaactg ggtgatcgga gtgctgctct ttttcttcac cttattcctg
     3181 agcttcaccg gctatctcct gccctgggac cagatcgcca tctgggctat cacagtcggc
     3241 accaacctcg cgccgtatac ccccttgttg ggtgatgccg tgtacaaggt catcgtcggc
     3301 ggctcggcgg tctcacagct aaccctggtg cgcttctatg tggggcatgt gatcttgttc
     3361 ccgctggtgc tatccttgct gctggctgtg catttctggc gtattcgtaa ggatggcggc
     3421 gtcgctggcc caccgcctcc gtctcgtaga gaggttgaag cgcgggttga accgactgcg
     3481 gtcggctctg ctcgctgata gcatactcgg cgtcgcctgg gcgctaacgt ccggcgatgg
     3541 ctctcttgag aggaatacta ctatgtcaat tgaacaacaa gaaccgaaag cgcagacaca
     3601 ggcgggggca acgcagcgcc gttatcgtac cctggccgtc gtgaagcaag aagcgatcac
     3661 gcgcgtcgaa aggccgctcg aagattcggt cttcgtatgg ccgcacctgt tagtgcgtga
     3721 atttttcgct tcggcgatag tgatggtcat gattaccgtt ctatcgattg tgatcgacgc
     3781 gcccctgcgc gagccggcca acccgaacgt gacgcccaac ccggcgaaag ccccgtggta
     3841 cttcctgggc ttgcaggagt tgttgcacta cttctcgccc aacgttgccg gtgtgctggt
     3901 gcctggcctg acgctgactg ccctgatcgt gctgccctat gttgatcgca accccagccg
     3961 cgcctatgcc gaccgcaaag tcgcgattgt gaccttcacg atgttcctgg tgttctgggc
     4021 gtttatcacc atcgccggca gcttcttccg cggtcctggt tggatatggg tatggccctg
     4081 gcagcacatg tactttgacc tgtaaccgca ctctagatag agaggatttc tatgagtctc
     4141 gatactcctc caatctcacc gcagcagtac gaagtcttgc acggcggtgg cgcagaggcc
     4201 gcggagatcg cccaacagcg cctgtcgcgg cggcgtttcc tgcgcaggtc gatgctggcg
     4261 gtgtggggtt tgtcagcgac ggcggcagta gccggagggc tctacatgac gtatcccaat
     4321 ctggccggcc agttcggctc tgatttgacg atcggcacga aatcgcaatt cccagccgcc
     4381 aaaccgcagg actacaaaat aaatcaggca ggcatttttt acgttcaaga ggcgaagacc
     4441 tatttatcgc acattgcagc cggtacgccg acactcttga atggttcaag tctgagcgac
     4501 cagctcgatt ctgaaagctt cgtcaaggac aaggacggct ccctgtgggt cgcgctttac
     4561 cagcgctgcg tccacctggg ctgtaccgtg ccgttccgcg acgactgcgt cagcttcaaa
     4621 tgtccgtgtc acggctcgca ctacaatgtc gatggcgagt acctggatgg cccggctccg
     4681 cgcagcctgg accgtttttc catgtccttt agcggcgata gtgtggtcgt ttcgacgggt
     4741 acgctcaata ataaagttcc tcaccctgac tctacaaccc gtatcattgc ggtgccgggt
     4801 gtacaatgca gcgtctagct cgtatgcgca cgaaccatca tgcgcagcgt gttgaggaat
     4861 gtaaggaagt gttgttatga ttagtcccgt acaggcagcc gtaggtatta tcgtagcgct
     4921 tcttgctgta ggcgcgttgc tctatacgtt ttattcacgc accaactcgg tagagaagac
     4981 cggctacggc gcgctcatga tgctgtcgat tatctcattg atgatcccga tcttctggat
     5041 cgtagagtct aacggcgaga cgatatcgca gctccagcag cacaataccg ccgtgaatcg
     5101 tggcgcgtca ctttttgcgc agtactgttt tcaatgtcac ggcatctttg gccagggggt
     5161 cgtcggaccc aagttgaacg ggaacaccgc tgtcaacaat ctgagcgatt cggacatcct
     5221 gcgtatcatc agcggcggca tctacgatcc tgccaatcca acgaccgcct tgatgcccgc
     5281 gtggagcgac cagtttggcg gtccgctcac cgaggatcag attcagtatt tatttcaact
     5341 cattcgctca gcagacccgg cgtacctgtc gaaaaacggt ttcccgaccg gccctggcac
     5401 caacggcttc gaccaggttc ccggtctcgt gcagcagagc cagccaattc cgtacagcac
     5461 cgccatcgcg caggctactg ccggttcggg catcgggcag ttcccattcg tggatctgac
     5521 cagtcggcca agcatcatca tccaaatcat cgattcaccc accggtgccg cgtgtaatcc
     5581 tgcctgttat gccgtcgccg acccgaaaaa tgctagccag ttgattatcg acccgaacat
     5641 caaggtcaag gttggcacga aaatcacctg gattaataag agtaagacta ttcacactgt
     5701 tacctcgctt gtgggtgaag tcccaaccgt tcaggttccg gcaaaggttt ttgactctgg
     5761 actcaccacg ccgatggcgg caggtacagg tgtttactcg tatacagtcg ctacacaagc
     5821 gtacacattt aacagcaacc atatcgtagt gtactattgt cagttccacc cctcgatggt
     5881 ggctgccatt accgttactc agtaatgtca tcgcctggcc tttcgccgcc cgttgtaagg
     5941 aatgcgcagt gccaggcatt agcctgatcg ctggtcatat cgcgcacagc gcgcgggtat
     6001 ggtagaaggg atcacgaagt atgcaacaaa cgcaacaaag ggagcaggga gaccgcatat
     6061 cctgggcgcg tgccatgatc tttggcgtgg gcttcttctt tattgctgcc ctgctggtgg
     6121 ggcagttccc ctcctatgtg tattatcaga tgacctcttc gtccttgacc gccattgaag
     6181 gcggcttcct ggcgctgggg gctatttgtc ttgctggatt tgtcgtcatt caagtcattg
     6241 tctttctctt cgatcccaag ccagtcattc cgccgatcgt ctttagcgtg ttgggcctgg
     6301 gtttgagcgt tgttggcgtg gcgatcatgc tgtgggctgt cgtgaccggc aaccagggtt
     6361 tcccaaatgc cagcaccaac atagcgcccg tcttgaacgg caaattcctg tggttcgagc
     6421 cgggcgatat cgacatagtc atgcttggcc ttgtcattat gggcgttggc gtcgcaatgc
     6481 tcttttttag cgccctggcg tatcaggagc agcgcaatcc cgaccggcgc gatctcggca
     6541 cgactccagc cattcgcgcg cttatctggg ttgggttgac catgctggcc gccttcctgc
     6601 tcttctatac cttcttcagc gatacgcaac ttgcagccca gatataccca cagtgtccca
     6661 tcgccattaa tgcagcgaaa ggttgtcctg gaccgatcac gggcctgttt atcgtgcgta
     6721 ccatctttaa caccttcctg ggcattgcta ttttttgcac gctgggcgcg ttcgcgctgc
     6781 gcctgcatta cctgatgcgt ccggtgcgca aacgcacgat gtccagtctc tatatgatcg
     6841 gcatcaacct ggctcagttt ggcgcgatct tcctggtcgc ctggctggtt ctgtatcctt
     6901 tgatcgcctg gatgcattcc tggacattca tcggcctggg tgactatctc actctatgcg
     6961 cgaagaaatc ggctatcccg caaagctgcg ccttctccgc gcaggcgggc tatattctgg
     7021 ctgccgtggt ctgcggcggc tcctttaccc tcttgatggc cgccattgcc gtctggaaga
     7081 cgaggcgcaa tctcgtcgtc atcagtggcg tcacgctgac cgccgtgctt tccctggcaa
     7141 ccctggtagt gcataccaac cctgacgagg tgctgattgc cgcgttgctc tgtggcggcg
     7201 gacttggcct ggctgccatc tggacgagcg tcgcccggcg tgaattcgcc gtcgttggcg
     7261 aaaacaacct gggctgtgtc ggtcagtggc tcgtgcttgg cacctgcctg ctcatttaca
     7321 tcgcggcctt cgccttcttc tccctacccg tcttcgtc
//
LOCUS       DASZAK010000778         5718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_54367, whole genome shotgun sequence.
ACCESSION   DASZAK010000778 DASZAK010000000
VERSION     DASZAK010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54367"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VGT44_13735"
     CDS             complement(<1..846)
                     /locus_tag="VGT44_13735"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEV2581910.1"
                     /translation="MNKKERVDAALRGEAVDRVPASMWGHDFEREWDPHTLAEAMVEN
                     YTRYDWDYMKVNPRACYHVEGWGVKVHPSGEKYKAPVFEDTPIKSASDWKRIRTLDAD
                     QGALGEQLKALQLINHSVGYDAYFVQTIFCPLGVAKYLAGNKLDPVLHTIREDRNAMH
                     TALRVITETFTSYAIACLDEGASGIFYATNGWASEGMLSQDQYREFGEQYDLEILEAI
                     QRHSKLNILHNCGTRIHFDLLAGYPVHALSWAATLAGNPDLAEGKKRSGKAVMGGISE
                     KTTLKD"
     gene            complement(876..2888)
                     /locus_tag="VGT44_13740"
     CDS             complement(876..2888)
                     /locus_tag="VGT44_13740"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581911.1"
                     /translation="MMQNKASGRPGHIAAILLVCLVLLSSCVFGGSGPTGTSTPTKVV
                     AGSPSAGGNLPSPTAVGLASLLQTEQLLLMTPHPLRDLYSLARRLKLHTPAPIPHVGR
                     TTPLNEQVGQEDMFWINNSDTRAYARIRAQLVYITPHVYMYVEDGQHVDLAALELSAN
                     LFEQSTYVTDRATYGSEWSPGIDDDVHLTILNAVGLGSGVGGYFSSEDEYPTIINPYS
                     NEREMFYVSLDGGETPGTPDYNSTLAHEFQHMIHWYQHPVDLSWTNEGMSVLAQHING
                     FSAGGLDQDFVQKPDTQLNDWSDDINADLEHYGAGYLFMDYFAEHYGGYGVLKELLQD
                     PTSPPVNFDDVLAKHGYTDRFFDVLEKWYVANYVEDSTIGQGQYGYPTIHLPGVTPQH
                     TIQSYPTSEADTVHQYAAEYYAVQPSGKSGTLSISLRGAPTVRIVGNNPTGSSQEWWS
                     NRYDNMDSTLTRSFDLTSLAGKSVSLKFSTWFDLEPQYDYVFVEVSTDGLNWTTLKGN
                     YTTTSNPNGANWGNGYTGESGGGAAPQWVQEHMDLTPYAGKKIQLRFEEVTDDAVNYQ
                     GFAIDQISIPALGFQDAVTSDNGWVSTGFIRSNNILPEHFSLQALVYQGSSFTLQTMR
                     VDLASGTGTLSIPNFGSQVTRVVLVVSAYAAETTLLAQYRLNVNVT"
     gene            complement(2888..3607)
                     /locus_tag="VGT44_13745"
     CDS             complement(2888..3607)
                     /locus_tag="VGT44_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2581912.1"
                     /translation="MEKDPFDQRDMTILVVDDEARLRDVVSMNLEMEHYAVLEAANGL
                     EALEQLRDGLPDLVVLDVQMPEMDGFETLRHIREVSTVPVIMLTVLQTEQDKIRGLDL
                     GADDYLAKPFSPRELLSRIRALLRRSHMPTPARKTEIVVDDNLKIDFARREVIVRGRK
                     VVLRPTEYRLLYHLVSNAGRLLTHETLLSKVWGREYRDEAHYLRLYITYLRQKIEEDP
                     AHPKYILTERGIGYRFKELEG"
     gene            complement(3607..5205)
                     /locus_tag="VGT44_13750"
     CDS             complement(3607..5205)
                     /locus_tag="VGT44_13750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2581913.1"
                     /translation="MSWSQAREDGRDQLTILIGSAIRLLSGEAGVFVVSNKAYDPLSG
                     DEYIPYRLSEDGCAHLLAYTRGREQPDMARPLLVGLLPMVLVSGLGLQDVPLEEPGAC
                     PLEYCSLLVYDQAGICGQLYYLRLSGRQSCFEQVGDGGGENATLPLFIAHVAAGIRSA
                     MKAQLLLKEQNRLSAVIQQSAEGILTVDTALRIVDFNPAMERLTGWLESEVLGRYYYE
                     VLLPQDRHGKALGAQDSLILQAFASEGNVRREMTIAARDGQRFDVAVTASSVRSSKDE
                     AIGGILTVRDITREREEEERRSTFISVISHELQTPIAIIKGYASTLTRAALGDAQLDP
                     DMLRSRLQAVEEEADRLNKLVSNLLYASRIQAGGLQMDIAPLDLFSLVQTVAMRLRVK
                     SPDVTVRLDLAPNLPTVVADRDRIEEVLQNLLDNAVKYSPRQRVITVSARATSDEVIV
                     SISDRGMGISVRDQEEIFERFQRVGGSQVRSTPGAGLGLYICRAIVEAHGGRIWVESV
                     LHQGSIFSFSLPREEKARLPMVVF"
     gene            5401..>5718
                     /locus_tag="VGT44_13755"
     CDS             5401..>5718
                     /locus_tag="VGT44_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581914.1"
                     /translation="METTVHPDYSNIVGVFKEREPAEQALEALKQAGFKEEQLTVYHQ
                     DEVPTDAAELTSATRFLIHVRAEGREQEAVAILTKHGSNNADLPTGTKLAHGSLISSD
                     DAVN"
BASE COUNT         1301 a         1808 c         1428 g         1181 t
ORIGIN      
        1 gtctttcagc gtcgttttct cgctgatgcc gcccatcacc gccttgccag aacgcttttt
       61 cccctcagca aggtcaggat tgcccgccag tgtcgcggcc cagctgagcg catgtacggg
      121 ataccccgcc agcagatcaa agtgaatgcg cgtgccacag ttgtgcagga tattcaactt
      181 gctgtgtcgc tgaatcgcct ccagaatctc caggtcgtac tgctcgccaa actcgcggta
      241 ctgatcctgc gacagcatac cctcgcttgc ccagccattc gtcgcgtaaa aaatgccgct
      301 cgccccctcg tccaggcagg caatcgcata actggtaaag gtctcggtaa tcacacgcag
      361 cgcggtatgc atggcgttcc gatcctcacg aatggtatgt agcaccgggt caagcttatt
      421 ccccgccagg tatttcgcta caccaagcgg gcagaaaata gtctgcacaa agtaggcatc
      481 atagcctacc gaatggttga tcaattgcag cgccttgagc tgctcgccca gcgcgccctg
      541 atcggcgtcc agggtgcgaa tgcgcttcca atccgaagcg ctcttgatag gcgtatcctc
      601 gaagaccggc gctttgtact tctcccccga cggatgcacc ttgacgcccc atccttcaac
      661 atggtaacac gcgcgcgggt tcaccttcat ataatcccaa tcatagcgcg tatagttctc
      721 gaccatcgcc tccgccagcg tatgcgggtc ccattcgcgt tcaaaatcat gcccccacat
      781 gcttgccggg acacggtcaa ccgcctcgcc acgcagcgcg gcatcaacac gttccttttt
      841 attcatccga catatccttc catatataca tgtccctacg tgacattgac gttcaatcga
      901 tattgcgcca acagggtagt ttcggcggca tacgccgaaa ctaccagcac tacgcgcgta
      961 acttgcgagc caaagttagg aatgctcaac gtacccgttc cgctcgcgag atcaacccgc
     1021 attgtttgca atgtaaacga gctgccctgg tacaccagcg cctgcaggct gaagtgttcg
     1081 ggcaagatgt tattcgagcg aatgaagcca gtgctgaccc acccgttatc cgaggttaca
     1141 gcatcctgga agcccaaagc cggaatgctg atctgatcga tagcaaagcc ctggtaatta
     1201 accgcatcat cggtcacttc ctcgaagcgc aattgaatct ttttcccggc atagggtgtc
     1261 aaatccatgt gttcctgcac ccactgtggc gccgcgcctc cgccgctttc gccggtgtag
     1321 ccattgcccc agttcgcgcc attggggttg cttgtcgttg tatagttccc tttgagcgtc
     1381 gtccagttca aaccatccgt ggaaacctcg acgaaaacat aatcgtactg cggttccaga
     1441 tcgaaccagg ttgaaaactt cagcgagact gacttacccg ccaggctcgt caggtcaaag
     1501 ctgcgggtaa gcgtactatc catgttgtca tagcgattgc tccaccattc ctgcgaagaa
     1561 ccggtcgggt tattcccaac gatacgtacc gtcggcgcac cccgcaagct gatcgatagt
     1621 gtgccactct tgcccgacgg ctgcacggca tagtactcgg cggcatactg gtgaaccgtg
     1681 tccgcctccg acgtaggata actctggatc gtatgctgtg gcgtgacgcc gggcaggtga
     1741 atagtggggt agccgtactg tccctggccg atggtcgagt cctccacata attcgccacg
     1801 taccacttct ccagcacatc gaagaaacga tccgtgtagc catgtttggc cagcacatca
     1861 tcgaagttca ccggcggcga tgtcgggtct tgcagcagtt ccttaagaac gccatagccg
     1921 ccataatgct ccgcgaagta gtccatgaac aaatagcccg cgccataatg ctccaggtca
     1981 gcattgatat cgtcgctcca atcgttcaac tgcgtgtcag gcttctgcac aaaatcctgg
     2041 tcaagaccgc ccgccgaaaa gccattgata tgctgcgcca gcaccgacat gccctcgttg
     2101 gtccagctca ggtcaaccgg gtgctggtac caatggatca tatgctggaa ctcgtgcgcc
     2161 agcgtactgt tataatccgg tgtgccgggc gtctcgccac catccaggct cacataaaac
     2221 atctcgcgct cgttgctata gggattgatg atggtagggt actcatcctc cgaagaaaag
     2281 tacccgccga caccgctgcc cagcccaacc gcgttgagga tggtcaaatg cacatcgtca
     2341 tcgattcccg gcgaccactc actgccgtag gtagcgcgat cagttacata ggtcgattgc
     2401 tcaaagaggt tcgcggacaa ctccaacgct gcaaggtcaa catgttgccc atcctcgaca
     2461 tacatgtaca cgtgcggcgt aatatagacg agctgcgcac gaatacgcgc atacgcgcgt
     2521 gtatccgagt tattgatcca gaacatatcc tcctgcccca cctgctcgtt cagcggcgtc
     2581 gtgcgcccga catgcgggat cggcgcaggg gtgtgcagct tgaggcgacg cgccaggcta
     2641 tacaggtcgc gcaaaggatg cggcgtcatc aacaaaagct gctctgtctg caacaaggac
     2701 gccagcccca ccgccgtcgg cgacggcaaa ttaccacccg cagatggcga acccgcaacc
     2761 actttcgttg gtgtcgatgt acccgtcggc ccgctgccac caaacacgca cgaggagagc
     2821 aggacaaggc aaacgagaag aatggcggcg atgtgaccag ggcgaccact tgccttattt
     2881 tgcatcatca cccttccagc tccttaaacc gataaccgat gccgcgctcg gtcaggatat
     2941 atttgggatg cgccgggtcc tcctcgatct tctgccgcag gtacgtgatg tacagccgca
     3001 ggtagtgcgc ctcgtcgcgg tattcacgac cccagacctt tgagagcagt gtttcatgcg
     3061 tcagcaggcg gcccgcgttg ctcaccaggt gatacagcag gcgatattcc gttgggcgca
     3121 ggacgacctt ccggccgcgc acaatcacct cgcgccgcgc gaaatcgatc ttcaggttgt
     3181 catcgacgac aatctccgtc ttgcgagcgg gggtcggcat atgcgaacga cgcagcagcg
     3241 cccgaatgcg cgaaagcagt tcgcgcgggc tgaacggctt ggcaagataa tcatccgcgc
     3301 ccaggtcaag tccgcgtatt ttatcctgct ctgtttgcag caccgtcaac ataatgaccg
     3361 gcacggtcga gacttcgcgg atatgccgca gcgtttcaaa gccatccatt tcaggcatct
     3421 gtacatccaa cacgactaaa tctggcaggc catcgcgcaa ctgctccaac gcttccaggc
     3481 cattcgcggc ctccagcaca gcataatgct ccatctccag gttcatgctc accacatcgc
     3541 gcagccgtgc ctcatcatcc acaaccagga tggtcatatc tcgttgatcg aacgggtcct
     3601 tctccattaa aacaccacca ttggtagacg cgccttttct tcgcgcggca aactgaacga
     3661 aaatattgag ccttgatgca aaacgctctc tacccagata cgtccgccat gcgcttcgac
     3721 aatggcgcga cagatatata aaccgagtcc ggcccccggc gtcgaacgca cctgggaacc
     3781 accgactcgc tgaaaccgct caaaaatctc ttcttgatcg cgcactgaaa tgcccatgcc
     3841 tctatcgctg atgctcacaa tcacctcgtc gctcgtcgcg cgagccgaaa cagtgatcac
     3901 acgctgccgt ggagaatact tcaccgcgtt atccagcaag ttttgcagca cctcttcgat
     3961 ccggtcgcgg tccgccacga ccgttggcaa gttcggcgcg agatccagcc tgaccgtcac
     4021 atcgggactt ttgacgcgca gacgcattgc caccgtctgc acaaggctga aaagatcgag
     4081 cggcgcgata tccatttgta gtccgcccgc ctgaatacgc gacgcataca gcaggttgct
     4141 gaccagcttg ttcaggcgat ccgcctcctc ttccactgcc tgcaagcgcg aacgcagcat
     4201 gtccgggtcg agctgcgcat cccctagagc cgctcgcgtc agtgttgagg catagccttt
     4261 gatgatagcg atgggcgtct gcagttcgtg cgagatcacc gaaataaagg tggaacgccg
     4321 ctcctcctct tcgcgctcgc gcgtgatatc gcgcaccgtc agaatgcctc caatcgcctc
     4381 gtccttgctc gaacgcacgc tcgacgctgt caccgccacg tcaaagcgct gtccatcgcg
     4441 cgctgcaatc gtcatttcac ggcgcacgtt cccctcgctg gcgaatgcct gcaagatcaa
     4501 agagtcttgc gcccccaacg ccttcccgtg gcgatcctgt ggaagcagca cctcataata
     4561 atatcgcccc agcacttcac tttcaagcca gcccgtcaat cgctccatcg ccgggttaaa
     4621 atcgacgata cgcagcgccg tatcgaccgt caggatacct tccgcgctct gctggatcac
     4681 cgccgaaagg cgattttgct ccttcagcag caattgcgcc ttcatcgccg agcgaatgcc
     4741 cgccgcaacg tgcgcgatga agagcggcag cgtggcgttc tcgccccctc catctccaac
     4801 ctgctcaaag caagactgcc tccctgacag gcgtaggtaa tagagctgac cgcagatgcc
     4861 cgcctggtca tagaccagca acgagcagta ctcaagcggg caggcacccg gctcctctaa
     4921 aggaacatcc tgcaatccca gcccggacac cagcaccatc ggcagcaatc cgaccaggag
     4981 cgggcgagcc atatccggtt gctcgcgacc tcgcgtgtag gccagcaaat gtgcgcatcc
     5041 atcctcgctc aaacggtatg gaatatattc atcaccgctc aacggatcgt atgccttgtt
     5101 cgagacaaca aaaacacccg cttcgccact taataaacgg atcgcgctgc cgatcaggat
     5161 ggtcagttga tcgcgtccat cctcgcgcgc ctgcgaccag gacatggaga atccctctac
     5221 ccactcagga gtaacagata acgatgctat tgtaacacac ctcgtccaat ccgcaaaaga
     5281 actcatcggc tcctaagggg gggttggcta cagatggtat ctacacccat caactgcaac
     5341 cgagcaatgc cctgactcgt gatacaaata ttcacgttga gaaaggagag agaaatactc
     5401 atggaaacaa ctgtacaccc cgattattcg aacatcgtag gcgtattcaa agaacgcgaa
     5461 ccggcggaac aggcgctcga agccctcaaa caggctggtt tcaaggaaga acagttgacg
     5521 gtctaccatc aggatgaggt gccaacagac gctgctgaat tgacgtccgc gacacgtttt
     5581 cttatacacg tcagagcaga ggggcgagaa caagaagccg tcgcgatcct gaccaaacat
     5641 ggatcaaaca acgccgacct tcctaccgga accaaactgg ctcatggctc tctcatctcc
     5701 tcggatgatg cagtcaac
//
LOCUS       DASZAK010000779         4422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_125522, whole genome shotgun sequence.
ACCESSION   DASZAK010000779 DASZAK010000000
VERSION     DASZAK010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125522"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="VGT44_13760"
     CDS             complement(<1..320)
                     /locus_tag="VGT44_13760"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2581915.1"
                     /translation="MKITFLGHIGLFIETRHGSILCDPWFNPAYFASWFPFPSNEAID
                     RSMLANPTYLYVSHLHHDHFDPQFLQDNISKDATVILPDYPLNLLERRLRDLGFTKFL
                     QTKNA"
     gene            complement(334..1527)
                     /locus_tag="VGT44_13765"
     CDS             complement(334..1527)
                     /locus_tag="VGT44_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEV2581916.1"
                     /translation="MLELDRLKSDIDELVPGMVALRRDLHEHPELAFEEVRTSNIVAQ
                     RLRALGLEVQTGVAKTGVVGLLRGRPGSQDARTIAIRADMDALPIHELNEIEYRSQVD
                     GKMHACGHDGHTTILLAVADILTKRQDELAGNVKFVFQPAEEVVGGAAPMVKDGVMQG
                     VDGVIGLHLFSNYTHGRVGVRSGPVFASADKLILTVRGKGGHGAMPETTVDPIVIAAY
                     IVTALQTLISREISPFTPAVITIGRIMAGTAFNIIPETAEMPGTMRSFSQEQREKLLR
                     RIREIAQGVASAMGGACDVEVVDGCPACVNDAGMTELVRNAAVAAVGEQRVDTGEEVV
                     TSGSDDMAEFLEAVPGCYFIVGARNPAKDAKYPHHHPRFNIDEDAMPIAVEVLSRAAL
                     DFLKP"
     gene            1646..2764
                     /gene="ada"
                     /locus_tag="VGT44_13770"
     CDS             1646..2764
                     /gene="ada"
                     /locus_tag="VGT44_13770"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HEV2581917.1"
                     /translation="MYEDRYWQAVMERDSGVDGQFVYAVRSTGIYCRPSCPSRRPKRE
                     EVVFFQQPMAAEEAGFRACKRCRPRENAADPQVELAQRVCDAIEQRLDDMPGLAELSS
                     QVNVSPYHLQRVFKRVMGISPRQYAEARRLDRFKEQLKEGETVTQALYGVGFSSSSRL
                     YERAPDQLGMTPTVYRRGGPGMQITYTIVDCSLGRLLVAATDKGICSVCIGDDDTKLE
                     AALLHEYPRAEMRRDDGTRYGDDFHAWVEMLVQHLGGDQPHLDLPVDVQATAFQWRVW
                     QELRAIPYGATRSYSQVAQAIGQPTAARAVARACATNPIAIVVPCHRVVRENGQMGGY
                     RWGIERKERLLAQEASGKEDEIDSRFSSSLAVAKPPLA"
     gene            2860..2997
                     /locus_tag="VGT44_13775"
     CDS             2860..2997
                     /locus_tag="VGT44_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581918.1"
                     /translation="MMIALLVVIVLVSLLDLGALRWGVSSVDGADSPEWERRHLWYGF
                     H"
     gene            3023..4000
                     /gene="yedA"
                     /locus_tag="VGT44_13780"
     CDS             3023..4000
                     /gene="yedA"
                     /locus_tag="VGT44_13780"
                     /inference="COORDINATES: protein motif:HMM:NF008432.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HEV2581919.1"
                     /translation="MGERHFERVVSLGQTAVDAVNQDDRQSTTTSEPVASSARNRLMI
                     LLSLLTLYTLWGGTYLGMRIALEGFPPFFVAAFRQFIAGVILYFFLRLRGAPSPTRAQ
                     WLGSMVVGGLLLLFGNGLVVFAEQYVSSGLAALALGAIPLWASLFSGLFGRWPRPVEW
                     CGLGVGFAGLVLLNLENGFHANLLGAVLLLIAPMSWALGSILSQRLPSPKGLMASAAQ
                     MLMGGALLFIVGFGTGERMTAFPGPRPLFAMIYLVVGGSLIAFSAYGYLLRTVRPALA
                     TSYAYANPLVAVGLGVALAGEQVGLLEVLAMLTILSGVALVSLGRERRG"
     gene            complement(4001..>4422)
                     /locus_tag="VGT44_13785"
     CDS             complement(4001..>4422)
                     /locus_tag="VGT44_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEV2581920.1"
                     /translation="AEGAEVVAVSARLEAELAELPEEDAAEYLEALGLPSLGAERVIQ
                     VGYRLLNLITFLTAGEDEVRAWTVTKGAKAPEAAGKIHSDIERGFIRAEVTRYEDFMA
                     SGGSFAAAAKKGLQRLEGKEYVIQDGDIAHFRFNVGK"
BASE COUNT          797 a         1222 c         1443 g          960 t
ORIGIN      
        1 gcgttcttcg tctggagaaa tttcgtgaag cccaggtcgc gcaggcggcg ttccagcagg
       61 ttcagcggat aatcgggcag aatgacggtg gcatccttcg agatgttatc ttgcaagaat
      121 tgcgggtcga agtgatcgtg atgcaggtgc gaaacgtaca ggtaggtcgg gttcgccagc
      181 atcgagcggt cgatggcttc gttgctgggg aaagggaacc aggaggcgaa gtaagccgga
      241 ttgaaccagg gatcgcacag gatactgcca tgccgggttt cgataaacag gccgatatgt
      301 ccgaggaacg ttattttcaa aaggcgaact ccttcagggt ttgagaaaat cgagcgccgc
      361 gcgcgatagc acctcgacgg cgatgggcat ggcgtcctcg tcgatattga aacgcgggtg
      421 atggtgcggg tacttcgcgt ccttggccgg gtttctcgcg cctacgatga agtagcagcc
      481 gggtacggct tcgaggaact cggccatgtc gtcgctgccc gatgtcacga cctcctcgcc
      541 ggtatccacg cgctgctcgc cgacggcggc gacggcggcg ttgcgcacca gttcggtcat
      601 gcctgcgtcg ttgacgcagg ctgggcagcc gtccactacc tcgacatcgc aggcgccgcc
      661 catagcgctc gcgacgcctt gcgcgatttc gcggatgcgc cgcagcaatt tctcgcgctg
      721 ctcttgacta aaagaacgca tcgtgccagg catctccgcc gtttcgggga tgatgttgaa
      781 ggcggtgccg gccatgattc tgccaattgt gatgaccgct ggagtgaacg gcgaaatctc
      841 gcggctgatg agcgtttgca gcgccgtcac gatataagcg gcgatgacga tggggtcaac
      901 ggtagtttcg ggcatggcgc catgcccgcc tttgcccctg acggtcagga tcagcttgtc
      961 cgcgctggcg aagacagggc cagagcgcac gccgacacgc ccatgcgtgt aattgctgaa
     1021 gaggtgcaga ccgatcacgc catccacacc ctgcatgacg ccatctttga ccattggcgc
     1081 ggccccgcca acgacttctt cagcgggctg gaagacaaac ttgacgttac ccgccagctc
     1141 gtcctggcgt ttcgtcaaga tatcagcgac cgccagcaag atggtggtat gtccatcatg
     1201 cccgcaggcg tgcatcttgc cgtccacctg cgagcgatac tcgatctcgt tgagttcgtg
     1261 aatgggcagc gcgtccatat cggcgcgaat agcaatcgtt ctggcgtcct ggctgccggg
     1321 tctgcctcgc aacaggccga ccacgcccgt tttggccacg ccggtttgca cctccagccc
     1381 cagggcgcgc aggcgctgcg ccacgatgtt cgaggtgcgc acctcctcga aagccagttc
     1441 tggatgctcg tgcaggtcgc ggcgcagcgc caccataccg ggtaccagtt catctatatc
     1501 ggatttcaag cggtcaagtt cgagcatggt tcctccatga tgcggctgtc ctggcacaca
     1561 ttcagagcaa gaaacggata gattctttcc tcttcatatc ctatcatatg aacagaaggt
     1621 ttggaacaat caggaggaag cgacgatgta tgaagatcga tactggcagg cggtgatgga
     1681 gcgtgatagc ggcgtggatg ggcaatttgt gtatgccgtg cgttcaaccg gcatctattg
     1741 caggccatcc tgtccatcgc ggcggccaaa acgtgaggaa gtggtgtttt tccagcagcc
     1801 gatggcggcg gaagaggcgg gttttcgggc ctgcaaacgc tgccgcccgc gcgagaacgc
     1861 cgctgatcca caggtagaac tggcgcagcg cgtgtgtgac gctatcgagc agcgcctgga
     1921 tgatatgccg gggctggcgg aactgtcctc gcaggtcaat gtcagtccct atcacttgca
     1981 gcgtgtcttc aaacgggtga tgggcatttc cccacgccag tacgcggagg cgcgccgctt
     2041 agatcggttc aaagagcaat tgaaggaggg agaaacggtg acgcaagcat tatatggagt
     2101 aggtttcagt tccagcagcc gactgtacga acgcgcgcct gaccagcttg ggatgacccc
     2161 gacggtctac cggcgcggcg gtcccggtat gcagattacc tatacaattg ttgattgctc
     2221 cctgggtcgc ctactcgtgg cagcgaccga taagggcatc tgttccgtgt gtatcggcga
     2281 cgatgacacg aagctggaag ctgctctgct gcacgagtat cccagggccg agatgcggcg
     2341 cgatgatggg acacgctacg gcgacgactt ccatgcctgg gttgagatgc tggtgcagca
     2401 tctcggcggg gatcagccgc acctcgattt gccggtggat gtgcaggcga cggccttcca
     2461 gtggcgcgtc tggcaggaat tgcgggcgat cccatacggc gcgacccgct cctacagcca
     2521 ggttgcgcag gctatcggcc agccgacagc cgcccgcgcg gtggcacgcg cctgtgccac
     2581 caatcccatc gctatcgtcg ttccgtgcca ccgcgttgtg cgcgaaaacg gccagatggg
     2641 cggctatcgc tggggcatcg aacgcaagga acggctgctg gcgcaggagg cgagcgggaa
     2701 agaagatgag attgactctc gtttttcttc cagtttggcc gtagcaaaac cgcccctggc
     2761 ctgatactat agaaagtgaa gtgtagggac aataggttgt atataaaatt tgtagaaaag
     2821 aacatagtaa aacaattggc tcaatagccg gaggaggata tgatgatagc tctactggtg
     2881 gtgatcgttc tggtgtcctt gcttgatctt ggcgcgctgc gctggggagt ttcgagcgtt
     2941 gatggcgctg atagtcctga gtgggagcgg cggcatcttt ggtacggttt tcactagata
     3001 ctagattacg agcaacggta atatgggaga acgacatttt gagcgagtcg ttagcctggg
     3061 tcaaacggcg gtggacgcgg tgaaccagga tgatcggcaa tcaacgacga catcggagcc
     3121 ggttgcgagc agcgcgcgga accggctcat gattctttta tcgctgctga cgctctatac
     3181 cctgtggggc ggcacttatc tggggatgcg gattgcgctg gaaggtttcc cgccgttttt
     3241 cgtggcagca tttcgccagt tcatcgccgg ggtgatcctc tatttctttt tgcggctgcg
     3301 cggcgcgcca tcgcctactc gcgcgcagtg gttggggtcg atggtagtgg gcgggctatt
     3361 gctgctcttc ggcaatggcc tggtggtgtt cgcggagcag tatgtttcgt cgggactggc
     3421 ggcgctagcg ttgggcgcga tccccctgtg ggcttcgctg ttctccggcc tgttcgggcg
     3481 ctggccgagg ccggtcgaat ggtgcgggct gggcgtcggc ttcgccgggc tggtgttgct
     3541 gaacctggag aacggatttc acgcgaattt gcttggggcg gttctcctgc tcatcgcgcc
     3601 tatgagttgg gcgctgggtt ccatcttgag ccagcgtcta ccctctccca aggggctgat
     3661 ggcgagcgcg gcgcagatgt tgatgggcgg cgcgctgctc ttcatcgttg gcttcggaac
     3721 gggggagcgt atgacggcct tccctggccc gcgccccctg tttgctatga tctacctcgt
     3781 cgtgggcggc tctctgatcg cctttagcgc ctatggctac ctgctgcgca cggtgagacc
     3841 cgccctggcg accagctatg cctacgccaa cccgctcgtg gcggttggcc tgggagtggc
     3901 gctagcgggc gagcaggtcg gtttgctgga ggtattggcg atgctgacga ttttgtcggg
     3961 cgtggcgctg gtgtcgctgg gacgggagag aagagggtga ttacttcccc acgttaaagc
     4021 ggaaatgcgc gatatcgcca tcctgaatga catattcttt tccttcaagg cgttgcagac
     4081 ctttcttggc ggcggcggcg aacgaaccgc cgctggccat gaagtcctcg tagcgcgtca
     4141 cttcggcgcg gatgaagccg cgttcgatgt cggagtggat tttgcctgcc gcctcggggg
     4201 ctttcgcgcc ttttgtgacg gtccaggcgc gcacttcgtc ctcgcccgcg gtcaggaagg
     4261 tgatgaggtt gagcaggcgg tagccgacct ggatgacgcg ctctgctcca agactgggca
     4321 ggccgagcgc ttcgaggtat tcggcggcgt cctcttccgg tagttcggcc agttcggcct
     4381 ccaggcgtgc tgagactgcc acgacttctg cgccttcagc tc
//
LOCUS       DASZAK010000780         1757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_195270, whole genome shotgun sequence.
ACCESSION   DASZAK010000780 DASZAK010000000
VERSION     DASZAK010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195270"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1032)
                     /locus_tag="VGT44_13790"
     CDS             complement(79..1032)
                     /locus_tag="VGT44_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2581921.1"
                     /translation="MPNFSGRDPESFGFEEIIYSKRDWVATVTMNRPQSYNSYTWRTL
                     QEMTQAFRDVAWDDQIAVLVLTGAGDKAFCTGGDVKEYASEYTQRPHDYWKWMGLFTE
                     CHDMLRNIGKPTIARLNGMVVGGGNEFNMACDLAIAADHVRIRQVGTEVGSVAAGGAT
                     QWLPIMVGDRRAREILWLCEWIDADTALKWGLVNQVVPYAELDDAVATMAAKLVDKFP
                     ECMRYTKQQTNFWKDFAWSQTIGHARDWLALHYSSLEPYEGMSAFVEKRRPDHLGIRN
                     RAVSGESSEFLWGPYAQTCPNCGASGLPAQFTYCGSCGTAL"
     gene            complement(1037..1264)
                     /locus_tag="VGT44_13795"
     CDS             complement(1037..1264)
                     /locus_tag="VGT44_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581922.1"
                     /translation="MTQIRSARLQAEGWTRRFTALGRRLNEAAELYNELGYEVQLEPV
                     NLEEEETMGAESCKDCFVTMQARTIYTRPKE"
     gene            complement(1301..>1757)
                     /locus_tag="VGT44_13800"
     CDS             complement(1301..>1757)
                     /locus_tag="VGT44_13800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEV2581923.1"
                     /translation="LDIQKMSIRPMRPADLAVVVELDAHAFGSARPAYFERRLAVLDT
                     AGADTRAIFLVAVYRDQLIGFVMGTLAYGEFGLTQVTAILDSIAVHPHYQHQGIGQQL
                     TEAFMKQSARQGASAVYTLVNWDNWTLLKVFHALGFVLASTIPLERRID"
BASE COUNT          372 a          484 c          477 g          424 t
ORIGIN      
        1 gcgctcagga tgacaggggc acgcaggatg atggcgccca ttgtcatcct tcgctgcgct
       61 caggatctcc tgttttgatt acagggctgt gccacagctt ccgcaatagg tgaactgggc
      121 gggtaagccg ctggctccac aattaggaca ggtctgagca taggggcccc agagaaattc
      181 gcttgattcg ccgcttacgg cgcggttgcg tatgcctaga tgatcgggcc gacgtttttc
      241 gacgaaggcg ctcatgcctt cgtatggctc taatgaggag tagtgcaggg caagccagtc
      301 gcgggcgtgg cctatggtct gggaccacgc aaaatctttc cagaagttcg tttgctgttt
      361 ggtgtagcgc atgcattcag ggaatttgtc aaccagtttg gctgccattg tggcgacagc
      421 gtcatcgagt tccgcatagg gcaccacttg attgaccagt ccccacttca gagcggtatc
      481 tgcatcgatc cactcgcaaa gccacaagat ctctctggcc cggcgatcac caaccataat
      541 cggcagccac tgcgttgctc cgccagcggc gacgctgccc acctcggtac cgacctggcg
      601 aatacgcacg tggtcggcag caatggcgag atcgcatgcc atgttaaact cattgccacc
      661 acccacgacc atgccgttga gccgggcgat ggtgggcttg ccgatgttgc gcagcatgtc
      721 gtggcattct gtgaaaagtc ccatccactt ccagtagtca tgcgggcgct gtgtgtactc
      781 ggaggcatac tccttgacat caccaccagt acagaaagct ttgtctccag caccggttag
      841 cacgagtact gcgatctgat catcccaggc gacatcacga aacgcctgtg tcatctcttg
      901 caacgtgcgc caggtatagg aattgtagct ctgcggacgg ttcatggtga cggttgctac
      961 ccagtcacgt ttgctgtaga taatttcctc aaagccaaag ctctcagggt ctctcccact
     1021 aaaattcggc atggtgttac tcctttgggc gggtataaat tgtgcgagcc tgcatggtga
     1081 cgaagcagtc tttgcagctc tctgctccca tagtttcctc ttcttcgagg tttactggtt
     1141 ccaactggac ttcatagcca agctcgttat agagttctgc agcttcgttc agtcgccgtc
     1201 ccagggcggt gaagcgcctg gtccagcctt ctgcttgaag tcgagcacta cgtatctgtg
     1261 tcatattcgt actcatcggc atcgtcctct ctcgatctgg tcagtcgatc cgtctttcca
     1321 gcggaatggt cgatgccaat acgaaaccta atgcatgaaa aactttcagt aaggtccaat
     1381 tatcccagtt caccagtgtg tagacagccg aggcaccctg gcgagcgctc tgcttcataa
     1441 aggcttcggt caattgctga ccaatgccct ggtgttggta gtggggatgg acagcaatcg
     1501 agtcaaggat agctgttact tgcgtcaggc caaactcacc ataggctagc gtacccataa
     1561 caaagcctat tagctggtcg cgatagacag ccaccaggaa gattgctcga gtgtcggcgc
     1621 ctgctgtatc gagcacggca agacggcgtt cgaagtatgc cggtctcgct gagccgaagg
     1681 cgtgtgcatc aagttcgact acaaccgcca ggtctgctgg tcgcataggt cgaatcgaca
     1741 tcttttgaat atcgagg
//
LOCUS       DASZAK010000781         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_125530, whole genome shotgun sequence.
ACCESSION   DASZAK010000781 DASZAK010000000
VERSION     DASZAK010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125530"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VGT44_13805"
     CDS             <1..638
                     /locus_tag="VGT44_13805"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2581924.1"
                     /translation="IARFTSGWFENLLLPVWLPAMPEVQARLERGALVADVGCGQGKA
                     LIKLAQTYPRSRFVGYDSFAPSIEQAKANAEAAGVSDRVRFEQRDVSEGLPEQYDVIT
                     TFDVVHDAVNPRGLLRAIRNALRPGGRYVCLEINSSDKLEENIGLLGAFFYSCSVLYC
                     MTTSLAHHGEGLGTTGIPESKMRELCAEAGFSSVRRVPMENPFNILYEVTL"
     gene            735..1064
                     /locus_tag="VGT44_13810"
     CDS             735..1064
                     /locus_tag="VGT44_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HEV2581925.1"
                     /translation="MALYMVQFAYTAEAWATLAKNPQDRSGPVRELAQKLGGRLVGFY
                     YCFGEYDGVGLIDLPDDTAAIATSVAAVAPGHIKAIKTTKLFTVEETLEAMRTVGSLA
                     FQGPSIG"
     gene            1114..1437
                     /locus_tag="VGT44_13815"
     CDS             1114..1437
                     /locus_tag="VGT44_13815"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV2581926.1"
                     /translation="MYGTIGRLRIKSGMEGQFRQFIEGQAPAFEAGQVPGFVVSYTYR
                     MDANPNDYYVVAVFESREAYWANAQSPEQDARYRQWLPLLDGEPEWHDGEIVGGYALQ
                     RPGQK"
     gene            1552..>2025
                     /locus_tag="VGT44_13820"
     CDS             1552..>2025
                     /locus_tag="VGT44_13820"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV2581927.1"
                     /translation="MTNKLKLMCVLAHPDDESLGNGGVLAKYAAEGVETYLVTATRGE
                     RGWAGSESDYPGVAALGRRREAELLVAAEVLGIRRVEFLDYIDGDLDQALPAEAVAKI
                     VGYLRRVKPDVVVTFGPDGAYGHPDHIAICQFTTAAIVEAASPDAPSHRDLAPHRV"
BASE COUNT          445 a          544 c          633 g          403 t
ORIGIN      
        1 gcatcgcgcg ctttaccagc ggctggttcg aaaatctgct gctgccagtc tggctgccgg
       61 ccatgcccga agtgcaggcc aggctggaac gcggtgctct ggtggccgat gtgggctgtg
      121 ggcagggaaa agcgctcatc aagcttgccc agacctaccc ccggtcacgc ttcgtcggct
      181 acgacagctt cgcgccatcc attgagcagg cgaaggccaa tgccgaggcg gctggcgtgt
      241 ctgatcgcgt gcgcttcgag cagcgggacg tatccgaagg cctacctgag cagtacgatg
      301 tgatcaccac cttcgacgtt gtgcatgacg cggtcaatcc tcgcgggcta cttcgagcga
      361 tccgcaatgc gcttcgaccc ggcgggcgct atgtctgcct ggaaatcaac agctcagata
      421 agctggaaga aaacatcgga ctgctcggag ctttcttcta cagttgtagc gtgctctact
      481 gcatgaccac ctcgctcgcc catcatggcg aagggttggg taccaccggc atccccgagt
      541 cgaaaatgcg tgagctatgc gcagaagcag ggttcagcag cgtacgccgt gtgccgatgg
      601 agaatccctt caatatcctc tacgaggtca cgctctagca gcgccatgcc tcgcatctgc
      661 aaggaagtcg cgcagtacag atcaggcacc acgaaatcag aaatgaacga gtcgccggag
      721 aggagacgaa tgttatggct ctttacatgg ttcagtttgc gtacaccgcc gaagcgtggg
      781 caacgctggc gaaaaaccca caagaccgga gtggacccgt gagggaactg gcacagaagc
      841 tcggaggtcg cctggtcggc ttctactact gttttgggga gtacgatgga gttgggctca
      901 ttgacctgcc tgatgatact gctgccatcg ccacgagtgt agcggcagtc gccccagggc
      961 acatcaaagc gatcaagacg actaagttat tcaccgttga ggagacgttg gaggcgatgc
     1021 gcacggtggg gagtctcgcc ttccaggggc catccatagg gtagatcatt cggatctctc
     1081 atgcatgagt catagcaaag aaaggagaca aacatgtacg gaacaatcgg gcgtttacgc
     1141 atcaaatcag gaatggaagg ccagttcaga cagttcatcg aggggcaagc ccctgcgttt
     1201 gaagctgggc aggtacctgg gttcgtagta agctacacct atcgtatgga tgctaacccg
     1261 aacgactact atgtggtggc ggtattcgaa agccgagagg cgtattgggc gaacgcgcag
     1321 agtcctgagc aggacgcccg ctatcgccag tggcttccac tgcttgatgg tgagcccgaa
     1381 tggcatgatg gcgaaatcgt cggcggctat gcgctgcaga ggccaggtca gaaatgagtg
     1441 agggaccgcg cgttcttgca gcaagagacg caaggccgta ttcgatgact tcatcctagt
     1501 gctggtcggc aaagctggca agggatgctc gagaacgcga ctggagcaac tatgaccaac
     1561 aaactgaaat tgatgtgtgt gctggctcat cctgacgatg aatcgctggg caatggcggt
     1621 gtgctcgcga agtacgccgc cgagggagtc gaaacctatc ttgttacagc gacgagaggc
     1681 gagcgcggct gggcaggctc cgagagcgac tatccagggg tggcagcgct gggtaggaga
     1741 cgcgaggccg aattgctggt ggcggctgaa gtgcttggta ttcgccgtgt tgagtttctg
     1801 gattacatcg atggcgacct ggaccaggcc ttgccggcag aggcggtagc aaagatagtt
     1861 gggtatctac gccgcgtgaa acccgatgtt gtcgtcacct tcggcccaga tggtgcctat
     1921 gggcatccag atcatatcgc catctgccag ttcaccacgg ctgctatcgt cgaggcagct
     1981 agtccagacg ctccatccca ccgcgacctg gctccgcatc gtgtc
//
LOCUS       DASZAK010000782         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_517980, whole genome shotgun sequence.
ACCESSION   DASZAK010000782 DASZAK010000000
VERSION     DASZAK010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517980"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VGT44_13825"
     CDS             <1..1148
                     /locus_tag="VGT44_13825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581928.1"
                     /translation="QKTQLASPPSQPPVVVPNPATPVLPIMPLNNPASNPGAIRAAQP
                     TEIAPALNTPDSSRSVGNAAVDDSQATALVTPESQPVQFHLPIQRQLPQSQQPAEAQP
                     VRQPQPAQPQRPVSQPGTIASSSARSGLSRRAFLFGGLVTAGVVVAGGITLFALQNGN
                     HGLGGNGTTPTPATTGTHPVTGPTVLAQDDFQRNNQQFWGTASDGSHQWQQDAATSNF
                     FSINNQMGQIQVPQNGTGGELTAILGQSFANGEVFVKGTVSNFNNSQLSAILRWTDDN
                     HYYKVFLQGNKFNLIKRMNTVTPISKNIQFTTQAGVTYSLRFRIVGNTMEAKAWQSDQ
                     QEPTGWMFTVTDNNQQYNSGNGGIRTNVSAGVTVDITSFELLTANSL"
     gene            1246..2913
                     /locus_tag="VGT44_13830"
     CDS             1246..2913
                     /locus_tag="VGT44_13830"
                     /inference="COORDINATES: protein motif:HMM:NF018332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEV2581929.1"
                     /translation="MQQQEGQSAVQDESSVAGNIGRQEVVEAYLYSRRETIFTMVGVL
                     SVVFLSILDQTIVGTAMPHIVADLQGFNSIAWISTAYLLTSTVPIPIYGKLSDMFGRK
                     PIMLFGAVIFLAGSALSGAAQSMNELIACRAFQGLGAAALQPIAIAIIGDLFPPRERG
                     KWQGVSASIYALAAIIGPLAGGWLTDNASWRWVFYVNLPVGILAMLALIFVMPALRSN
                     ARQLSIDYAGAALLILGTVPLLLGFSLAGSQYAWLSPQVIALFVGAVVVLTALVLYAR
                     RLERRGKEAVFEPGLITNSPRIFGISLLVTAIFSVSLYGAAFAIPLFAQGVLGTSATD
                     SGLILVPFMLASIGGSVLSGLTLTLTGKYKWIAILGLVIDIAGTILLVRLDAHSGYTA
                     LLIAMLVLGLGVGSGLAVYSVVVQNVYPEKIGAASSTLVFFRQLGGTIGLAAMGSALT
                     SSYVPAFHAALPNALRQLLPANILGAFDNPLVLLSPGSLSQIRAGFAGYGAQGTTVYN
                     LLLEAVRTGLAQSVHNVFVLSLGVILTGLVAVIFLKEIPLRERREVP"
     gene            complement(2918..3517)
                     /locus_tag="VGT44_13835"
     CDS             complement(2918..3517)
                     /locus_tag="VGT44_13835"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2581930.1"
                     /translation="MSPRTEETNARIREEQKERILEAAKQIFAHKGFTDTKMADIAAE
                     AKVSYGLAYHYFANKEAIFTNLLHQALLGIIELMQRARMQPGTPWDRLRWLTEQIMQG
                     AQEEPEFEMLVLQALTNSAVPEEIRAKVLRESRLSHAALQQLIEEGQAAGQVVAGDPD
                     LLTTTFEWCIQGMVFGISYNNYAPTHLPDVEMALRMLKA"
BASE COUNT          749 a         1060 c          987 g          852 t
ORIGIN      
        1 tgcagaagac gcaactggcg tcgccacctt cgcagccgcc agtcgtggtt cctaatcctg
       61 ctacgccggt gttaccaatc atgcccttga ataacccggc gagcaatcca ggcgcaatac
      121 gcgcggctca gccaacggag atcgctcccg cgctgaatac gcccgactcg tcacggagtg
      181 taggaaatgc tgcggtagac gatagccagg cgacagcgct ggtgacgcct gagagccagc
      241 cagttcaatt ccatttgcct atccagcgac aactgccgca atcgcagcaa ccagccgagg
      301 ctcaaccagt ccggcaacca caaccggcac agccacaacg cccagtctct cagcctggaa
      361 cgattgcctc gtcttccgcc aggagcggcc tatcgcgccg cgccttcctc tttggcggcc
      421 tggtaacggc aggcgttgtt gtggcgggcg gaataacact cttcgcattg caaaacggga
      481 atcacggcct gggaggcaat ggcacaacgc ccacaccagc aacgaccggc acgcatcctg
      541 taaccgggcc aaccgtcctc gcccaggatg atttccagcg caataaccag caattctggg
      601 gaaccgcctc ggatggtagc catcaatggc agcaggacgc ggcgacgtcg aactttttct
      661 caattaacaa tcagatggga cagatacagg ttcctcagaa cgggaccggt ggggagttga
      721 ctgctattct ggggcaatct ttcgcaaatg gggaagtctt tgtgaaaggc acggtcagca
      781 atttcaacaa ctcacaactc agcgctattt tgcgctggac agatgataac cactactaca
      841 aggtgttcct ccagggaaat aagttcaatc tcatcaaacg catgaatacc gttaccccga
      901 tcagtaaaaa tatacaattc acgacccagg caggcgttac atacagcctt cgcttccgca
      961 tcgtcggcaa tacgatggaa gctaaagcct ggcagtctga ccagcaagaa cctactggct
     1021 ggatgttcac agttaccgac aataaccagc aatataactc gggcaacggc ggcattcgca
     1081 ccaatgtaag cgccggtgtg acagtcgata tcacctcgtt tgagctacta acggcgaaca
     1141 gcctgtaaac tgacttttct tccacttgac agccagctta aaatcgtgta catttgtctt
     1201 gtatattgat tgataaatca atcaaaagac aaagagagag aaaaaatgca gcaacaagaa
     1261 ggccaatcag cagttcaaga tgaaagttct gtagcaggga atatcgggcg gcaagaagtt
     1321 gttgaagctt atctgtattc caggcgtgaa accatcttta ccatggtagg tgtcttgtcg
     1381 gtcgtcttcc tgtctatact ggaccagacg attgtgggaa cggctatgcc gcatatcgtg
     1441 gcagacctgc aagggttcaa cagcatcgcc tggatttcta ctgcctacct gctcacctcg
     1501 acggtgccga tcccgatcta tggcaaactg tccgacatgt ttgggcgcaa gccaatcatg
     1561 cttttcggcg cggtcatctt cctggccggt tccgcgctct ccggggcggc gcagtccatg
     1621 aacgagctga tcgcctgcag agcgtttcag ggcctgggtg cagccgcttt gcagcctatc
     1681 gccatcgcta ttatcggcga cctgttcccg cctcgcgagc gcggcaaatg gcagggagtg
     1741 agcgctagca tctatgccct ggcggctatc atcggccccc tggcgggtgg ctggcttacc
     1801 gacaatgcct cctggcgttg ggtcttctac gtcaatctgc cagtgggcat cctggcgatg
     1861 ctggcgctga ttttcgtgat gcccgcgttg cgcagcaatg cccgacagct ttccatcgat
     1921 tacgctggcg cggcgctgct gatcctgggt accgttccac tgctgcttgg cttttcgctg
     1981 gcggggtcgc aatacgcctg gctttcgccg caggtcatcg ctctgtttgt tggcgctgtt
     2041 gtcgtgctga ccgcactggt gctgtatgcc aggcgacttg agcgacgggg aaaggaagct
     2101 gtttttgaac cgggcctgat aaccaacagc ccgcgcatct tcggcatctc cctgctcgtg
     2161 accgccattt tcagcgttag cttatacggc gcggcctttg ctattccgct tttcgcgcag
     2221 ggtgtgttgg gaaccagcgc taccgatagc ggactgatcc tcgtgccgtt tatgctggcc
     2281 tcgattggcg gcagcgtcct gtctggccta acgctgaccc tgaccggtaa gtataaatgg
     2341 attgccatac tcggccttgt catcgatatc gcgggaacta tcctgctggt tcgcctggat
     2401 gcccattcag gatataccgc gctgctgatt gccatgctag tacttggcct gggggtcggg
     2461 tccggcctgg cggtctacag cgtcgtggta cagaacgtct atcctgagaa aatcggcgcg
     2521 gcttcctcta cgctggtgtt tttccggcaa cttggcggaa cgattggcct cgccgcgatg
     2581 gggtctgcac tcacatccag ttacgtcccg gccttccatg ccgcgctgcc gaatgcgctc
     2641 aggcaattac tgcccgcgaa catcctgggc gcattcgaca acccactcgt gctgctctcg
     2701 cccggttcgc tgtcgcagat ccgcgccggc tttgcaggct acggggcgca gggtacgacg
     2761 gtctacaatc tgctactcga agctgtcaga accgggctgg ctcagagcgt ccacaacgtc
     2821 ttcgtgttga gcctgggcgt tattcttacc gggttggtgg cggttatctt cctcaaggag
     2881 attcctctaa gggagcgccg cgaggttccg tagggggtta agcctttaac atgcgcagcg
     2941 ccatctcgac atcaggcagg tgggttggtg cgtagttatt gtagctgatg ccgaaaacca
     3001 tgccctgtat gcaccattca aaggttgtcg ttagaaggtc gggatcacct gccaccacct
     3061 gcccggcggc ctgtccttcc tcgatgagct gctgcagggc tgcgtggctc aagcgacttt
     3121 cgcgcaagac cttcgcgcgt atttcctccg gcacagcgct atttgtcagc gcttgcaaga
     3181 ccagcatctc aaactctggt tcttcctgcg ctccctgcat gatttgctcg gtcagccagc
     3241 gcaggcggtc ccagggcgtt cccggctgca tccgcgcacg ctgcatcaat tcgatgatac
     3301 caagcaaagc ctggtgcagc aagttggtga agatagcttc tttattggca aaatagtgat
     3361 aggccagccc gtagcttact ttcgcttcgg ctgcgatgtc cgccattttc gtgtcggtga
     3421 agcctttatg cgcaaatatc tgcttggcgg cctcaaggat gcgttccttc tgctcctccc
     3481 ggatgcgggc gttggtttct tctgtgcgag gtgacatgct atagcactct ttcggttgat
     3541 tgataaacca attataagct cttttgtaca cttgcgccaa ttatgcaact atccagataa
     3601 tgaaccacgc ccctttagca accgtgcgtt gacaactgct ttttccca
//
LOCUS       DASZAK010000783         2274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_195336, whole genome shotgun sequence.
ACCESSION   DASZAK010000783 DASZAK010000000
VERSION     DASZAK010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195336"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1074
                     /locus_tag="VGT44_13840"
     CDS             121..1074
                     /locus_tag="VGT44_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEV2581931.1"
                     /translation="MYFPTTRVLTILELLQSHARMSGPELARRLEVNTRTVRRYIIML
                     QDLGFPVEAERGRHGSYRLRPGFKLPPMMFTEDEALALTLGLLAARRLGLTSATPAVE
                     GALAKIERVLPAGLRERVQAVQETLILDFANAGPTPRTTIVVTLCTSMQQERRVTIRY
                     ASFNEETTERAVDLYGLVYRPGYWYAVGYCHLRQDLRVFRLDRIVEAEMSNETYTRPA
                     DFDAVEYVTHSLAMTPATWPVEVLLDASLEEARTYVPTVMAELEETSNGLLLRCSIGN
                     LDWFAHFLAGLPFSMTVRQPQELRVELQRLAARIAEMANRE"
     gene            complement(1080..1994)
                     /locus_tag="VGT44_13845"
     CDS             complement(1080..1994)
                     /locus_tag="VGT44_13845"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2581932.1"
                     /translation="MLTEVEQTTRGTASPAQPALPSPRLRNEALLALNTVIWGGTFLV
                     VKYEVKLSGPFTYLSICYAVGALTLALIFRRRLAHITRAELRGGLLLGLVLFAGYAFQ
                     TTGLQFTSVSKAGFITGLYVPLVPLFFFVFLRHRPAFTAIIGIALAFAGLFLLSINNQ
                     LTLSIGIGEVLIFGAAVAFAAHILLIGKLSHSVDMTNLAIIQLSLTSLLSLAVLPFAR
                     ELHTMPPAQFWLIAVLMGMGDIAYTLLVMNRVQQRVGGIRATLIYSMEPLWAALAGVL
                     LAGDVLSIPAWIGGGCILAGMIVGRLGQ"
     gene            complement(2019..2153)
                     /locus_tag="VGT44_13850"
     CDS             complement(2019..2153)
                     /locus_tag="VGT44_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581933.1"
                     /translation="MMELLILIALLGMLVVAAVLWGANSAETIDSPEWEKRHNRGLNI
                     "
BASE COUNT          475 a          664 c          676 g          459 t
ORIGIN      
        1 gcgctcagga tgacaggctc agtcgctgat cctggttgtg aaacttcata aattatcatt
       61 taaacaagtg taccatgtaa tgcggctatt tttcgcccgc ttttgtgata gactggtaat
      121 atgtattttc caacaaccag ggtgcttacc atccttgaac tgttgcaatc gcacgcgcgc
      181 atgagcgggc cggaactggc caggcggctg gaggtcaaca cgcgcacggt gcggcgctat
      241 atcatcatgc tgcaagacct gggctttccc gtagaggccg aacgcgggcg gcatggctcc
      301 tatcgcctgc gccccggctt caagctgccg ccgatgatgt tcaccgagga cgaagcgctg
      361 gcgctgacgc ttgggctgct cgcggcgcgg cggctgggcc tgacatcggc gaccccggcg
      421 gtcgagggcg cgcttgccaa gatcgagcga gtgctgcctg ccggcctgcg cgagcgcgtc
      481 caggcggtgc aggagacgct gattctcgac ttcgccaacg ccggtcctac acccagaaca
      541 acaattgtcg tcacgctttg cacctccatg cagcaggagc gccgcgtcac aatacgctat
      601 gcctctttta acgaggagac gaccgaacgc gcggttgacc tgtatggcct ggtttaccgt
      661 ccggggtact ggtacgccgt cggctactgt cacctgcgac aggacctgcg cgtctttcgc
      721 ctggatcgta tcgtcgaggc tgagatgagc aacgaaacct atacgcgccc ggcagatttt
      781 gacgcggtag aatacgtcac acactcgctt gctatgacac ctgccacctg gccggtggag
      841 gtcttgctcg acgcttccct ggaagaggcg cgcacctacg ttccgacggt catggctgag
      901 ctggaggaga cctccaacgg cctcctgctg cgctgctcta ttggcaatct cgactggttc
      961 gcgcattttc tggctggcct gccattctct atgaccgtgc ggcagccgca ggaattgcgc
     1021 gtcgagttac aacggctggc tgcgcgcatc gcagagatgg cgaaccggga ataaagtggt
     1081 tattgcccca gcctgccgac gatcatgccg gccaggatgc agccgccgcc gatccaggcg
     1141 gggatgctca gtacgtcgcc tgccagcagc acaccggcca gagcggccca gagcggctcc
     1201 attgaataga tcagcgtggc gcggataccg ccaacgcgct gctggacgcg gttcattacc
     1261 agcaaggtat aggcgatatc gcccatgccc atcagcacgg cgatcaacca gaattgggct
     1321 ggcggcatcg tgtgtagttc gcgcgcgaag ggtagtacgg ctaagctgag cagggatgtc
     1381 aggctcaact ggatgatcgc caggttcgtc atatccacgc tgtgagaaag cttgccgatc
     1441 agcaagatat gcgcggcgaa ggcgacggcc gcgccgaaga tcaacacttc gccgatgccg
     1501 atggataacg tcaactggtt gttaattgaa agcaggaata accccgcaaa cgcgagagct
     1561 atccctatga tggcggtaaa ggcgggccga tgccgcaaaa agacaaagaa gaatagcggg
     1621 acaagcggca catagagtcc cgtgatgaat cccgctttgc tgacactggt aaattgcagg
     1681 ccggtggtct ggaaagcata gccggcgaag agtacgaggc caagcagcag tccaccgcgc
     1741 aattcggcgc gtgtgatatg cgccagtcgc ctgcggaaga tcaacgccag cgtgagcgct
     1801 ccgaccgcgt aacagatgga caggtaggta aacggcccac tcagcttcac ctcgtatttg
     1861 accaccagga aggtgcctcc ccagatcacg gtgttcagcg ccagcagtgc ctcgttgcgc
     1921 aggcgaggag aaggcaatgc tggctgcgcc gggcttgctg ttccccgcgt ggtctgttcg
     1981 acttcagtaa gcattgcctt gctcctttcc tggtgtttct aaatgtttaa tccccggttg
     2041 tgccgctttt cccattcggg agaatcgata gtttcggcgc tattcgcgcc ccaaagcaca
     2101 gccgcaacta ccagcatacc aagaagcgcg ataagaatca atagctccat catgtcaaac
     2161 tccttgtgcc tgcttgagat gcaggtgctt ctgcggttca gtatggaaga atttctgcca
     2221 ccagcgttta tagactacct ggcgtgccag gcgctgctgc gcggcctgac gtaa
//
LOCUS       DASZAK010000784         1737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_54561, whole genome shotgun sequence.
ACCESSION   DASZAK010000784 DASZAK010000000
VERSION     DASZAK010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54561"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VGT44_13855"
     CDS             complement(<1..263)
                     /locus_tag="VGT44_13855"
                     /inference="COORDINATES: protein motif:HMM:NF014148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581934.1"
                     /translation="MARIRLAYVGGGSTRAAGTMASFVHQGENFDGSEVVLIDLNEEH
                     LRVVQTIAQKMARARGLDIVVTATTDRRAGLRDCDAVLTSFRPG"
     gene            complement(284..1372)
                     /locus_tag="VGT44_13860"
     CDS             complement(284..1372)
                     /locus_tag="VGT44_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2581935.1"
                     /translation="MSAKNEETGLSAVPETTQAENFAETPVGAPILEAVHLRKYFPLR
                     GVRLMGPQQAVHAVEDTTLALYPGRALALVGESGSGKTTVARLLARLYEPTSGDIRFR
                     GQVVQGSSGTLKTYRKRVQLVFQDPFASLNSVHDVRYHLSRPLRIHGHVRVKLQETSQ
                     ILSLLNRVSLSPAEQFISKYPHQLSGGQRQRVAIARALAAQPEVLLADEPVSMLDVSI
                     RLDVLNLLLKLKEEERLAVLFITHDIASARYFAEDTLVMYAGQSVEGGPSEDVIQQPK
                     HPYIRLLISAVPDPQHLGPGREKAAPLPARGEIPSLINPPTGCRFHPRCPHAMDICRE
                     RFPGRTDLGGGHWTNCFLYGEGQSQDYS"
     gene            complement(1369..>1737)
                     /locus_tag="VGT44_13865"
     CDS             complement(1369..>1737)
                     /locus_tag="VGT44_13865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV2581936.1"
                     /translation="ETATCQELYEQPRHPYSYGLLNSFPTMHGPRHRMTGIPGSPPDL
                     RDVPPGCAFHPRCPCAFGACSTQLPMLRPSTPETPRQLVACHLYDPRFNAGKDIPTNA
                     EFAKSYKAHYDAIQGVRSAE"
BASE COUNT          323 a          527 c          548 g          339 t
ORIGIN      
        1 cccgggcgga agctcgtcag cacggcatcg cagtcccgga gacccgcgcg ccggtcggtg
       61 gtcgctgtga ccacgatgtc caggccgcgc gcgcgcgcca tcttctgggc aatggtttgc
      121 acgacacgca gatgctcctc gttcagatca atcagcacga cttctgaacc atcgaaattt
      181 tccccctggt ggacaaatga ggccatcgtg ccagccgcgc gggtgctgcc tcctccgacg
      241 tacgccagtc gaatacgagc catgaatgat atactccttt tccttatgaa tagtcttgcg
      301 actgcccttc gccatacagg aaacaattcg tccagtgtcc gccgcccaga tcagtgcggc
      361 caggaaagcg ctcgcggcag atgtccatgg cgtgcgggca gcgcggatgg aaacggcagc
      421 cggtgggagg attaatcagg ctggggattt ccccgcgcgc gggtagcggc gctgctttct
      481 ctcttccagg ccccaggtgc tgcgggtcgg gcacggcgga aatcaacagg cgtatatagg
      541 gatgcttcgg ctgctggatc acgtcttcgc tcggcccgcc ctcgacgctc tgcccggcgt
      601 acatcaccag tgtatcttcg gcgaaatagc gcgcactggc gatatcatgc gtgatgaaga
      661 ggaccgccag gcgttcttcc tccttgagtt ttaagagcag gttcagcaca tccaggcgaa
      721 tcgagacatc cagcatcgag accggctcat cggccagcag cacctccggc tgcgcggcca
      781 gggcgcgcgc gatggcgacg cgctggcgtt gtcccccgct caactggtgc ggatacttgg
      841 agatgaattg ctcggcggga ctcaggctga cgcggttcag cagagacaat atctggctgg
      901 tctcttgaag cttgacgcgc acgtggccgt ggatgcgcag agggcggctg aggtgatagc
      961 gcacatcatg cacggaattg agcgatgcaa acgggtcttg aaagaccagt tgcacgcgct
     1021 tgcgataagt tttgagcgta cccgacgacc cctgcaccac ctggccgcga aagcggatgt
     1081 cgcccgatgt cggctcgtat agtcgcgcca gcagtcgtgc taccgtcgtt ttgccgctgc
     1141 cgctttcgcc caccagcgcc agcgcgcgcc cgggatagag cgccaatgtg gtatcttcga
     1201 ccgcgtgaac cgcctgctgc ggccccatca gtctgacgcc gcgcaggggg aaatacttgc
     1261 gcaggtgaac ggcttccagg atcggggcgc ccaccggcgt ttccgcgaag ttttcagcct
     1321 gcgtggtctc aggaaccgca gacaggccgg tttcctcatt tttcgcactc attctgcgct
     1381 cctcactccc tgtatggcgt cataatgggc cttatagctt ttggcaaatt cagcattggt
     1441 tggaatatcc ttacccgcgt tgaagcgtgg gtcatagaga tgacaggcaa ccagttgccg
     1501 cggtgtctca ggcgtcgatg gccgcagcat cggcagctgc gtcgagcagg ctccaaaggc
     1561 gcacggacag cgcgggtgaa aagcgcagcc tggcggcacg tcgcgcagat cgggcggcga
     1621 accggggatg ccggtcatac ggtggcgcgg cccgtgcatg gtcgggaacg agttcaacag
     1681 accatagctg taaggatggc gcggctgctc gtacagttcc tggcaggtag cagtctc
//
LOCUS       DASZAK010000785         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_481361, whole genome shotgun sequence.
ACCESSION   DASZAK010000785 DASZAK010000000
VERSION     DASZAK010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481361"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VGT44_13870"
     CDS             <1..337
                     /locus_tag="VGT44_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581937.1"
                     /translation="YVGLILGLQSLFQGVFKQNNDVAIVVSTLVIAALFQPLRHRIQR
                     IIDRRFYRRKYDAARTLAQFSASLRNEVDLQQLKESLVVVVEETMQPAHISLWMRPTS
                     RERIPNTNQ"
     gene            528..1898
                     /locus_tag="VGT44_13875"
     CDS             528..1898
                     /locus_tag="VGT44_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581938.1"
                     /translation="MNLSNTERKSASGAVDSSIANTRLTGSWLIVARVVWLALVVPSL
                     GLFVANLVAYDQQLQRACAGPFLCNLTGALTAKDLQAFSSGSISVSWYAALLTISLAL
                     IVSIWCGVGFLIFWRRSDDWLALLAAFFLVMFSITPSGNNPGFVILFAYPVLALPISL
                     VSFLGQISISVFFSLFPNGRLVPRWMGLVLLLYIIYAFFNNFPFPTSTFDMNWPVWLN
                     LLITLGFPGAIVISQIYRFRRVSTPLQRQQTKWILLGATVAVAVVIGLQLISFLILPS
                     QNPLGGGSGVILSYIFFVAFLLIPLSIGFSFLRYRLYDIDVLINRTLVYGALTGILAL
                     LYFGMVLGLQFLFDRIMGPSAADSPLILVGSTLVIAALFQPLRHRVQSMIDRRFYRSR
                     YDARRTIANFSASLRGEVDLAELSERLVAVVQETMQPTHVSLWLSQPRKVQSDFSGEI
                     SNSRSS"
     gene            1997..>2437
                     /locus_tag="VGT44_13880"
     CDS             1997..>2437
                     /locus_tag="VGT44_13880"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2581939.1"
                     /translation="MATFIQRLQHIRPGRAKKHAQIVEQPGAVSRSRKVETLPLDIAP
                     NDPLVAYFLSAPGTVEIDKLNLDSPALKALKVAGVQMVIPLVSQGELVGLLNLGPRRS
                     EQDYSTDDRTLLNDLATQAAPALRVAQLVREKQAQNLERERIEQE"
BASE COUNT          486 a          669 c          656 g          626 t
ORIGIN      
        1 ctatgtgggc ctcattcttg gcttgcaatc cctcttccag ggggtgttca agcagaataa
       61 cgatgtggcc atcgtggtct caacgctggt cattgccgcg ctcttccagc ccttgcgcca
      121 tcgcatacag aggatcattg atcgccgttt ctatcgccgc aagtacgatg ctgcacgcac
      181 gctggcgcag tttagtgcct ccttgcgcaa tgaggtggac ttgcagcagt tgaaagagag
      241 cctggtggtg gtggtcgaag agaccatgca gccagcgcat atctccttgt ggatgcgccc
      301 taccagccgc gagagaatac cgaacaccaa ccagtagtgg gtctgtgatc gcactgttta
      361 tcatcttcat ggagaagcaa agcattcctt tctattctat gcaaagcatg ttgcgaggtg
      421 gtctctaacg attgcgggac ggggatatac tatgatatgg atgagtatga cagaaacctc
      481 tctctgagtc gttgcgtttc tcttctctgc ttgtgaggga aagatgcatg aacctatcga
      541 atactgaaag aaagagcgca agcggagcgg ttgactccag catcgcgaat acgcgcttaa
      601 caggctcctg gctcatcgtg gcgcgcgtcg tgtggttggc gctggtagta cccagcctgg
      661 gactctttgt tgccaatctc gtggcgtatg atcagcagct ccaacgggcg tgtgctggcc
      721 cttttttgtg taacctcacc ggggcgctga cggcgaagga ccttcaggcg ttctctagcg
      781 gcagcatttc tgtgagttgg tatgcggctc tgctcaccat ctccctcgcg cttatcgtct
      841 cgatctggtg tggcgttggg ttccttattt tctggcgccg ctcagatgac tggttggcgc
      901 tgctggccgc ctttttcctc gtaatgttca gcattactcc ctctggcaac aatcccggct
      961 ttgtgatact ctttgcctat cccgtcctcg cgttgcccat cagcctggtg agctttcttg
     1021 gacagatctc cattagtgtg ttcttttcgc tcttccccaa tggacgactc gtcccgcgct
     1081 ggatgggtct ggtcctgctg ctctacataa tctatgcctt tttcaacaac ttccctttcc
     1141 ctacgtcaac gttcgacatg aattggccgg tgtggctcaa tctgctaata actcttggtt
     1201 ttcctggagc catcgtcatt tcgcaaatct atcgtttcag gcgcgtgtca actcctctcc
     1261 agcgccaaca gaccaagtgg attctcttag gcgcaacggt ggcagtagca gttgttatag
     1321 ggttacagtt gataagcttc ctcatcttac ccagtcaaaa tcccctgggt ggagggagtg
     1381 gagtgatttt gagttatatc ttcttcgttg cctttctctt gatcccgctc tccattgggt
     1441 tctcgttttt gcgctatcga ctgtatgata tcgacgtgct gattaaccgc acactggtat
     1501 atggcgcgct gacgggtatc ctcgccctac tctactttgg catggtgctt ggactacaat
     1561 tcctctttga tcgcatcatg ggacccagcg ccgcagactc tcccctgatc ctggtcggtt
     1621 cgaccctggt gatcgccgcg ctcttccaac ccttgcggca tcgcgttcag agcatgattg
     1681 accggcgctt ctatcgcagc aggtatgatg cccggcgcac gattgccaac ttcagcgcga
     1741 gtctgcgtgg cgaggtagac ctggccgagc tgagcgaacg gctcgttgcg gtcgttcagg
     1801 agacgatgca gccgacacat gtttcactat ggttatctca accacgcaaa gtacaatcag
     1861 atttttctgg agaaatatcg aattcacgga gttcgtaacg cattctgtaa cgttcaggat
     1921 acgccgatgc tatacaatag aaacagagaa cgttcaatca tcccgatttc aatgtcggga
     1981 tacgtggagc gcatagatgg ctacattcat tcaacgctta caacacatac ggccaggacg
     2041 ggctaagaag catgctcaaa tagtggagca gccgggtgca gtctcccgtt cccgcaaggt
     2101 cgagacgctt ccgctcgata tcgcgccgaa cgacccgctc gtagcatact ttttgagcgc
     2161 gccggggacg gtggagatcg ataaactcaa cctggactcc ccagcgctca aggcattgaa
     2221 ggtggcgggt gtgcagatgg tcattccgct ggtcagccag ggagagctgg tcgggctgct
     2281 caacctggga ccgcgtcgca gcgagcagga ctattcgacc gacgaccgca ccctgctgaa
     2341 tgacttggcg acgcaggcgg cgcctgccct gcgggttgct caactggtac gcgaaaagca
     2401 agcgcagaac ctggaacgcg agcgcatcga gcaggag
//
LOCUS       DASZAK010000786         5546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_19991, whole genome shotgun sequence.
ACCESSION   DASZAK010000786 DASZAK010000000
VERSION     DASZAK010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5546
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19991"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VGT44_13885"
     CDS             <1..1543
                     /locus_tag="VGT44_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2581940.1"
                     /translation="LDYAHSNSVTHGNLSLSNVLVNADLAVQIAGFGLKTMLETHRDR
                     RSSNSQAHLLSATGTFLGNADYIAPERVQGLSSGARADIYALGVMLYELLSGTSPFHG
                     ATALETALQRIQKPAPLLHAACPDLSEGFDLVIGKALALDPARRYQHAGEVARAYERV
                     LKTLGVAQSAPLSAPALPAKPALDQQTILTSGDTELEEDAHPSGKWQLMPPIITDKLP
                     ALEPTALKDTEPRQGAAQRQGKQIGDSLLGTDPFAWWTTTAEQTAPREAGTFGRTTQR
                     QPVRLTGPRGPRQAVRQDRRQAVKLIATGAVVAGVFAAGGISFARFAQSLKQTTQFSN
                     LPTNGSTTITRGATPTAGTTQAAHPSPTAGKTATPKATPTHAAQPTPTTQPTTQPTTQ
                     PTPKPTQPPPPPPTPTPGHTGTVIGQTSMPTNSAVKFTNPTDGQDSLLIHLQNGNFVA
                     CERACTHAGVPVNYDSGQMQIVCPAHGAIFDPLNGFAHLSGPGNGPLPTVTIRVNADG
                     TITTG"
     gene            complement(1547..1780)
                     /locus_tag="VGT44_13890"
     CDS             complement(1547..1780)
                     /locus_tag="VGT44_13890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581941.1"
                     /translation="MDSGNITHETTGFYTGLNQANLIGVVALGNTLDLYANGQRFASQ
                     TDSSAVGGEIGVLATAYTQPTEVAFSDAKVWTL"
     gene            2010..4037
                     /gene="argS"
                     /locus_tag="VGT44_13895"
     CDS             2010..4037
                     /gene="argS"
                     /locus_tag="VGT44_13895"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV2581942.1"
                     /translation="MPTTSQEPAMAIPGDLHDYLVSLIKQATITYLAQESIVVDIEQL
                     PVDLRFSAQASFGDYSVPLMKWAGKDKLGRPPLAIAQGLATVLQDIPTPGIAEITVTN
                     PGYLNFMLDRPATGEQIIERVLDAGADFGTGNIGAATKVIVEHTNINSNKAAHAGHLR
                     NSCLGDTLVRVLRSQGYKVEAENYIDDTGVQVADVVVAFDLLRQDLLEVPGGNDRLPG
                     ESFDYYCSRLYVAVGKAYEEHPELIEQRKAVLRAIEHTQQPYADMAADLSTKIVQAHL
                     ATMARLNVSYDLLTWESAILAAGLWNRTFEMLRDKGVLEHPESGPTAGCWVLPFGDGE
                     VQTEEGDRNSDKILVKSDGTATYTGKDIAYQLWKFGLADQLEAQFHFVPWGRQHDGRV
                     LWSMRPARADATDIPEADPLRFGHAKQVINVIDVRQSYPQQVVYESLRRLGYSEQAEH
                     STHFSYEVVALSASTAAALGVDTSDGRESYAMSGRKGIEVKADDLINAAMAKMQETRP
                     DLSSETAAILAASAIRYFMIRFNPQQMIALDMDEALRANGDTGVYLQYAYARANSILR
                     KLEEADYEIPEQASSLPDQLDQSEWELLRHIDAYPRTLAEAARQLAPNMLATYAYDLA
                     AHFSDFYEHTPPIVRETDERVKAFRAHLVLATVQSMDNVLRLMGFVPLERI"
     gene            4067..>5546
                     /locus_tag="VGT44_13900"
     CDS             4067..>5546
                     /locus_tag="VGT44_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 65 glycosyl hydrolase"
                     /protein_id="HEV2581943.1"
                     /translation="MTELSAFPVEEWSVREIGLNVDSLAQTESIFALSNGHIGLRGNL
                     DEGEPYGLPGTYLNSFFELRPLPYAEGGYAYPESGQTAINVINGKIIRLLVDDEPFDV
                     RYGELQAHERELDLRNGVLRRSVRWTTPSRRIVKISSVRLVSFTQRAIAAICYEVEPL
                     DAPIRVVVQSELVANETLPPQSQDPRVGAALASALESEEHAAKDTAGLLIHHTHASGL
                     LIGAAMDHHIEGPPGMLIECGSFPDVVRVSIAGRLKPGERLRIVKLISYGWSSLRSRP
                     AIHDQIVAALTAAQMTGWDGLLAEQRAYLDDFWARADVELEGDAEIQQAVRFALFHVL
                     QAGARGEGRPIAAKGLTGTGYDGHTFWDSEIFVLPVLTLTVPHATADALRWRHLILDQ
                     AKAHALQLGLRGAAFPWRTIRGAECSGYWPAGTAGFHINADIAYAVSYYLQATGDTAF
                     EEAVGLDLLVETARLWLSLGYLNTADNQFRIDGITGPDEYSAI"
BASE COUNT         1144 a         1789 c         1593 g         1020 t
ORIGIN      
        1 actagattac gcgcacagca acagtgtgac acatggcaac ctgagcctgt caaatgtgct
       61 ggtaaacgct gacctggccg tgcagattgc tgggtttggc ctgaaaacca tgctggaaac
      121 gcatcgcgac cggcgaagtt ctaactccca ggcccacctt ttgagcgcga ccggtacctt
      181 tcttggcaat gctgattaca tcgcgcccga acgggtgcaa ggtttatcaa gcggcgcgcg
      241 cgccgatatt tacgccctgg gtgtaatgct ctacgaattg ctgagcggca cctcgccctt
      301 ccacggcgca accgcccttg agaccgcctt gcagcgcata caaaagcccg cgcctctgct
      361 ccatgccgcc tgccccgacc tcagcgaggg cttcgacctg gtgatcggca aagcgctggc
      421 gctcgacccg gcgcgtcgct accaacatgc tggcgaagtc gcgcgcgctt acgagcgtgt
      481 tctcaaaaca ttaggggtgg cgcaatctgc gccactttcg gcacccgcgc tgcctgcgaa
      541 accagcactc gaccagcaga cgattctgac gagtggcgat acagagcttg aagaagacgc
      601 gcacccctca ggcaaatggc aattgatgcc accgatcatc accgacaagc tgcccgccct
      661 ggagcctacg gctctcaaag ataccgaacc tcgccagggc gcggctcaac gccagggcaa
      721 acaaattggc gattcgctgc ttggcacgga tcccttcgcc tggtggacga ctacggcgga
      781 gcagacagcg ccgcgtgaag cgggaacgtt cgggcgcaca acgcagcgcc agccggtgcg
      841 actgaccggc cctcgcgggc cgcgccaggc cgtgcgccag gatcgccgcc aggcggtgaa
      901 gctgatcgcc accggcgcgg tggtggcggg cgtcttcgcg gcgggtggca taagtttcgc
      961 ccgtttcgcg cagagcttga agcagacaac acagttcagc aatctcccca cgaatggctc
     1021 gaccaccatc acgcgcggcg ccacgccaac ggcgggaacc acccaggctg cgcatccatc
     1081 gccgacggcg ggcaagaccg cgacgccgaa agccacgccc acgcacgcgg cccagcccac
     1141 gccgactacg cagccaacaa cacaaccaac cacgcagcct acgccgaagc cgacccagcc
     1201 cccaccaccg ccgccaacgc ccacaccggg gcataccggc acggtaattg gccagaccag
     1261 tatgccgacc aattcagcgg tcaagtttac caatcccacc gatggccagg acagcctgct
     1321 cattcatctg caaaacggca acttcgtcgc ctgcgaacgg gcctgcactc atgccggcgt
     1381 ccccgtcaac tatgatagcg gccagatgca aattgtctgt cccgcgcatg gcgccatttt
     1441 cgatccgctc aacggcttcg cccatctctc cggccccggc aacggcccac tgcctaccgt
     1501 cactattcgc gtcaacgctg atgggacgat tacgacgggg tagaactcaa agcgtccaga
     1561 cctttgcatc actaaacgcg acctcggttg gttgtgtata cgcagtggcg agaacgccta
     1621 tttcacctcc gacagccgaa ctgtcggtct gacttgcgaa gcgctgcccg tttgcgtaga
     1681 ggtcaagggt atttccgagc gccacgacgc cgatcaaatt ggcctggttc aggccggtgt
     1741 aaaaccctgt agtctcgtgt gtgatattcc cggaatccaa tggatgaaaa cgactgccat
     1801 catagaagaa aaagaagtac cgaccatctg aaccgataga cagataaagg tatcttagca
     1861 gaatcaggtg caaatgtata gataaataga atgtgctcat cgagccgccc aggtacactt
     1921 gaccacccag gtaacatagg ttatagtaat ccagcgctca ttaaaacggt ttgtatcgct
     1981 gactcactcg tgagttgatg ggaggaacaa tgccaactac cagccaggag cctgctatgg
     2041 ccatccctgg agatctgcac gactacctcg tgtcgctgat caaacaggcg accatcacat
     2101 atcttgcgca ggaatcgatt gtcgtagata tcgaacagtt gccggttgac ctgcgcttct
     2161 ccgcgcaggc cagtttcggc gactattcgg taccgctcat gaagtgggcg ggtaaagaca
     2221 agctgggacg cccgccgctg gccatcgcgc aagggctggc caccgttttg caggatatac
     2281 ccacgccggg catcgccgag attaccgtca ccaatcccgg ctatctcaac ttcatgctgg
     2341 accggcccgc gaccggcgag cagatcatcg agcgcgtgct ggatgccggg gcggatttcg
     2401 gcacaggcaa tatcggcgct gccacgaaag tgatcgttga gcataccaat atcaacagca
     2461 acaaggccgc ccatgccggg catctgcgca actcctgcct gggcgatacg ctcgtacgcg
     2521 tcctgcgctc gcagggctac aaggttgagg cggaaaacta tatcgatgac acaggcgtac
     2581 aggtcgcaga tgtggtggtc gccttcgatc tactgcgcca ggacctgctg gaagttcccg
     2641 gcggcaatga ccgcctgccg ggagaatcct ttgattacta ctgctcgcgg ctttatgtcg
     2701 ccgtgggcaa agcctacgag gagcatccag agctgatcga acagcgcaaa gcggtgctgc
     2761 gcgccattga acatacccag cagccctacg ccgatatggc cgccgacctc tccacgaaaa
     2821 ttgtgcaggc tcacctggca accatggcgc gcctcaacgt gtcgtatgac ctgctgacct
     2881 gggaatccgc tattcttgct gccgggctat ggaatcgcac cttcgagatg ctgcgcgaca
     2941 agggcgtgct ggagcatccc gaaagcggcc caactgccgg ttgctgggta ttgccatttg
     3001 gcgacggcga ggtgcagacg gaggagggcg atcgcaacag tgataagatt ctggtgaaga
     3061 gcgacgggac ggccacctac actggcaaag acatcgccta ccaactctgg aagttcggcc
     3121 tcgccgacca gctcgaggcg cagttccatt ttgttccctg gggccgccag catgatgggc
     3181 gcgtcctgtg gtcgatgcgt cccgcgcggg cggacgcgac cgatatacct gaagccgacc
     3241 cactgcgttt tggccacgcg aagcaggtca tcaacgtcat cgatgtgcgc cagtcctatc
     3301 cgcagcaggt cgtctacgaa agcctgcgtc gcctgggtta cagcgagcag gctgaacact
     3361 ctacccactt ctcgtatgag gttgtggcgc tttccgcctc gactgccgcc gctctgggcg
     3421 tcgatacctc cgatggccgc gagtcgtatg ccatgtccgg gcgcaagggt atcgaggtca
     3481 aggccgatga tctgatcaac gccgccatgg cgaagatgca ggagacccgg ccagacctgt
     3541 catccgagac ggcggcgatc ctggcggctt cagccatccg ctacttcatg atccgcttca
     3601 acccgcagca gatgatcgcg ctcgatatgg acgaggccct gcgcgccaac ggcgataccg
     3661 gcgtctacct gcaatatgcc tacgctcgcg ccaacagtat cctgcgcaag ctcgaagagg
     3721 ccgactatga gataccagaa caggcctcaa gcctgcccga ccagctcgac cagagcgagt
     3781 gggagctgct gcgccacatc gacgcctacc cgcgcacgct ggccgaggcc gctcgccagc
     3841 tcgcgcccaa catgctcgcc acgtatgcct acgacctggc cgcgcatttc agcgacttct
     3901 acgagcacac accgcccatc gtcagagaga ccgatgagcg tgtcaaagcc ttccgcgccc
     3961 acctcgtcct ggccaccgtg cagagcatgg acaatgtgct gcggctgatg gggtttgtgc
     4021 cgcttgagcg catctaacac ttttagcttc tcattggagg tcgactatga ccgaactttc
     4081 tgctttcccg gtcgaggaat ggagcgtccg ggagataggg cttaacgttg attccctggc
     4141 gcagaccgaa tccattttcg ccctctctaa cggacatatt gggctgaggg gcaacctcga
     4201 tgagggggag ccatacggct tgcctgggac ctatctgaac tccttcttcg agttgcgccc
     4261 gctgccctat gccgagggag gatatgctta ccccgagtcc gggcagacgg ctatcaacgt
     4321 tatcaacggc aaaatcatac gcctgctcgt cgatgacgaa ccttttgatg ttcgctacgg
     4381 cgagcttcaa gctcacgaac gagaactcga cctgcgcaac ggggtgctgc gacgcagcgt
     4441 tcgctggaca accccgtcga ggcgcatagt caagatctct tccgtgcgcc tggtctcgtt
     4501 cacccagcgg gccatcgcgg ccatctgcta cgaggtcgag ccacttgatg cccctatacg
     4561 tgtcgtcgta cagtcggagc tggtcgccaa tgagacgctc ccacctcaaa gtcaggaccc
     4621 tcgcgttggc gccgccctcg cgtccgcact cgagagcgag gaacacgccg ctaaggacac
     4681 cgccgggctt ctgatccatc acacccacgc cagcggcctg ctcatcggag cagccatgga
     4741 ccatcacata gaggggcctc caggaatgct catcgaatgt ggaagcttcc ctgatgtcgt
     4801 gcgtgtttcc atcgcaggac ggctgaaacc gggtgaacgg ctgcgaatcg tcaaactcat
     4861 ttcctacggc tggtcgagcc tgcgctcgcg gcctgccatt catgaccaga tcgtcgccgc
     4921 gctgacagcc gctcagatga ccggctggga cggcttgctt gccgagcagc gagcctacct
     4981 cgacgacttc tgggcgcgcg cagatgtcga gctggagggt gacgcggaga tccagcaggc
     5041 ggtgcgcttc gcgctcttcc acgtcctgca agcgggcgcg cgtggagagg gtcggcccat
     5101 cgcggcaaag gggctgaccg gtaccggcta cgacggtcac acattttggg actcggagat
     5161 tttcgtcctg cccgtgttga cgctgaccgt gccgcatgcc accgctgacg ccctgcgctg
     5221 gcgtcacctg atcctcgacc aggcgaaggc acatgccctc cagttaggtc tgcgtggggc
     5281 tgccttcccc tggcgaacca ttcgaggagc agagtgttcg ggctactggc ccgccggcac
     5341 tgccggcttc catatcaacg cggacattgc ctacgctgtg tcgtactacc tccaggcaac
     5401 tggcgataca gcctttgagg aggcggtggg actcgacctg ctcgtcgaga cggcgcggct
     5461 gtggctttcc ctcgggtatc tcaacaccgc tgacaaccag ttccgcatcg atggcatcac
     5521 cggacccgac gagtacagcg ctatcg
//
LOCUS       DASZAK010000787         3687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_303431, whole genome shotgun sequence.
ACCESSION   DASZAK010000787 DASZAK010000000
VERSION     DASZAK010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303431"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1841)
                     /locus_tag="VGT44_13905"
     CDS             complement(<1..1841)
                     /locus_tag="VGT44_13905"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEV2581944.1"
                     /translation="MKVRSVYPVVFVPVVSIVVIVALLVIYLSPFTRAAPAVPRAALA
                     NAASAYTSTSQLVSAAQGSQRISLAIGLNLRNQDQLSSYLQQISSPTSPLYRRYLNAA
                     SFNALFGPLPQSEAAVVNYLRSQGLTITATYPNHLLVDASGTIAQVEQAFQVQINNYR
                     SASGTPFYANAAAPTLPVTIASLVSSVSGLDNTIRYQRHPLSSSLHGSLSGQSASACP
                     QPGSATTPTSYTPSQIAGAYDFNRLYNAGILGDGQSIGLLELDGFSSGDIAAYTACFG
                     GSHTTIQTIPIDGFNGKPGVDASEVELDMEMVLGLVPHLSSLRVYEAANALASYNDAW
                     ARIVSDGVSVVSTSWVFCEQGAGMSSEIQQENIFFQEAAAQGQTILAASGDLGANGCY
                     NPQTGTNQLPAVDDPASQPYVTGVGGTTLRLNFDSSYQSEQVWNDRAISDGASGGGVS
                     QVWQMPAWQQAPGVANAFSTGYREVPDVSINADPQTGYDVYCTVGGCSGAGSNHGWLV
                     FGGTSAAAPVWAAMIALANEYALKVNGYQLGFLDPSLYDIAHDASGTSYVSSFHDIVP
                     VQGGVNNNDYVGTGNTYPTASAYDLATGLGSYDAYNLAQNLNLLGQAT"
     gene            complement(2041..2610)
                     /locus_tag="VGT44_13910"
     CDS             complement(2041..2610)
                     /locus_tag="VGT44_13910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581945.1"
                     /translation="MGEQQGPVQRRVAQAHRVARQQILQAKYKNKGKQRQQPSAAQVT
                     VTIDESLPELQPLTKKIDRRTPRQMGKKLFLREQLITSVPVIETAAEKSKQEPIKFRS
                     PTEPINPTKPYDRDEFQDRVRAIIALIFVSTFVGTIVSSFVLAAFFSGNKDAWPNIKD
                     LIQIIVTTESGLLGAAVGFYFGEKVGRAS"
     gene            complement(2636..2845)
                     /locus_tag="VGT44_13915"
     CDS             complement(2636..2845)
                     /locus_tag="VGT44_13915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581946.1"
                     /translation="MAQMDGEELTVNFSGGAYDELKKLADAQNLDMSEALRRAIALNK
                     WVIETQKDAKILVERKGKIREIVSL"
     gene            3231..>3687
                     /locus_tag="VGT44_13920"
     CDS             3231..>3687
                     /locus_tag="VGT44_13920"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2581947.1"
                     /translation="MPDERRETSAEEVARVRSYLASQSVRRTPAQLVETLQDAHHQLL
                     ASVASIPDAHMRTTPRDGEWSAIDVLLHMRTIAAIDASSIPAVIERGEQPPDIQDAIT
                     FAPQTTTPSELLADLERLRAQLNVAVLQADPQAHLDITWSHPEFGAMHWR"
BASE COUNT          792 a         1054 c         1058 g          783 t
ORIGIN      
        1 gtggcctggc cgagcaggtt gagattttgc gccaggttat aggcatcgta ggagccgagg
       61 ccggtcgcca ggtcataggc gctggcggtg ggataggtat tgccggtgcc gacataatcg
      121 ttgttgttga cgccgccctg caccggcacg atatcgtgaa acgacgagac atacgatgtt
      181 cccgacgcgt cgtgcgcgat gtcgtacagg cttggatcga ggaagccaag ctggtagccg
      241 ttgaccttga gcgcatattc gttggccagc gcgatcatag cagcccagac gggcgcggct
      301 gccgacgtgc ctccaaacac cagccatcca tgattggagc cagctccgct acaccctccc
      361 accgtgcagt acacatcgta gcccgtctgc ggatcggcgt tgatcgagac atccggcacc
      421 tcgcggtacc cggtggagaa ggcattggcg actcccggcg cttgctgcca ggcgggcatc
      481 tgccagacct ggctcacgcc gccgccgctc gcgccatcac tgatcgcgcg gtcgttccag
      541 acctgctcgg attggtacga gctatcgaag ttgaggcgca gcgtcgtgcc acccacgccc
      601 gtgacatagg gctgcgaggc gggatcatcg acggcgggca gctggttcgt gcccgtctgc
      661 ggattatagc agccattcgc gcccaggtcg ccactggcgg ccaggatcgt ctgtccctgc
      721 gcggctgcct cctggaagaa gatattctcc tgctggattt cgctgctcat gcccgcgccc
      781 tgctcgcaga agacccagct cgtgctgaca accgacacgc catcgctgac gatacgcgcc
      841 caggcgtcat tgtaggatgc cagggcattg gcggcctcgt agacgcgcag cgaggagagg
      901 tgcggcacca gccccagcac catctccatg tcaagctcta cttccgaggc atcgacaccc
      961 ggcttgccat tgaatccatc gatggggatg gtttgaatgg tggtatggct cccgccgaaa
     1021 caggccgtgt acgcggcaat atctccggac gagaaaccat ccagttcgag cagcccgatg
     1081 ctctgcccat cgcccaggat gccagcgttg tacagcctgt tgaaatcata agccccggca
     1141 atctgcgaag gcgtatagga agtcggcgtt gtcgccgagc cgggctgcgg gcaggcgctt
     1201 gcgctctgtc cgctcaacga gccatgcagg ctggagctta aggggtgacg ctggtagcga
     1261 atggtattat ccaggccgct caccgaggag accagcgagg cgatcgtcac gggcaaggtg
     1321 ggcgcggcgg cattggcata gaaaggcgtc ccggaagccg agcggtagtt gttgatctgc
     1381 acctgaaacg cctgctcaac ctgggcgatc gtgccagaag catcgaccag caggtgattg
     1441 ggataggtcg cggtgatggt cagaccctgc gagcgcaggt agttcacaac cgccgcctcg
     1501 ctctgcggca atggcccgaa cagggcattg aacgaggccg cgttgaggta acgccggtag
     1561 agcggcgaag tgggcgagga aatctgctgg agatagctgc tcaattgatc ctggttgcgt
     1621 agattcagtc caatcgctag cgagatacgc tggctcccct gcgccgccga gacgagctgg
     1681 ctcgtgctgg tatacgccga cgctgcgttc gccagggctg cgcgaggcac agccggggcc
     1741 gcgcgcgtga acggcgacaa atagatgacc agcaatgcca cgattacaac gatacttaca
     1801 actggaacga aaacgaccgg atagactgag cgaactttca tacaatgcct ctttcaacgc
     1861 aaacatgctc agaagcttca gcaatacagg ctatagagac ttctcgatgc gttcaagaac
     1921 aatccccaga atccccaagg accccacaaa atgtcctaac cagattgaac ggaagcgcaa
     1981 gtattttcgg gggatttgag cgtgaggagg ggttagaagg tacttttggg tgaaagagat
     2041 tcagctcgcc cgtccgactt tttctccaaa gtagaagcca acggcagcgc ccagcagccc
     2101 tgattcggtg gtgacgatga tctgtatcag gtctttgatg tttggccagg catccttatt
     2161 tccgctgaag aaggctgcca gcacgaatga gctaacgatt gtaccaacga aggttgaaac
     2221 aaatatgaga gcgataattg ctcgcaccct gtcctgaaat tcgtcccgat cgtagggttt
     2281 ggtcgggttg attggttcgg ttggactacg aaattttatt ggttcttgct tacttttttc
     2341 agctgctgtt tctataacgg gcacagatgt gattaattgc tctcgtagaa agagcttttt
     2401 gcccatttga cgtggagttc gcctatcgat tttctttgtt aaaggctgaa gttcagggag
     2461 cgattcatcg attgtgacgg tcacttgagc ggcggaaggc tgctggcgct gtttgccttt
     2521 gttcttgtat ttcgcttgaa gaatctgttg gcgagcaaca cgatgagctt gcgcgactct
     2581 acgttgcact ggcccttgct gctcgcccat attgctactc actacatgtt acttcttaca
     2641 aactgacgat ctctcgaatt ttccccttac gctcaacgag gatctttgca tctttttggg
     2701 tctcgataac ccacttgttg agcgcaatgg ctctgcgaag ggcttcagac atatctaaat
     2761 tctgcgcgtc tgccagtttt tttagttcat cgtaagcacc gccggagaag ttcacggtca
     2821 gctcttcgcc atccatctga gccatctcgt agctcctttc tttcagacgg tacgcaagat
     2881 gctcatatat gcatcttctg tgcatagtat acgacaaaaa tgtgccaaag taaaacgctc
     2941 ctgttctggc gggtgtagga aatagttgta agtaatcgat caaacaagga tggcaacaac
     3001 ccctgtagga gccggtttac cgtgctcacc gccgattgat cggcgcgaat cctcattgat
     3061 ccgaattgca agactggccg atcaattggc ggtgagcacg gtaaaccggc tcctacagtg
     3121 attctgacgc ttgcttgcct acaagtcatt tctacactcg tcccagcatt gtcccaatca
     3181 gcatgacata atacaacata tagaacgacg aacaccgaaa ggaatagccc atgcccgatg
     3241 aacgacgcga gacgagcgcg gaagaggttg cgcgcgtgcg cagctattta gccagccagt
     3301 ccgtgcggcg cacacccgcg cagctcgtcg agacgttgca ggacgcgcac caccagctcc
     3361 tggcgtccgt cgccagcatc cccgacgcgc acatgcgtac aaccccgcgc gacggcgaat
     3421 ggtctgccat cgatgtgctg ctgcacatgc gcaccatcgc cgccatcgat gcgtcgagca
     3481 taccggcagt tatcgagcgc ggagagcagc cgccggatat ccaggacgcg attacgttcg
     3541 ctccgcaaac aacaacgccc tcggaactcc tggccgacct ggagcgcttg cgcgcgcagc
     3601 tcaacgtcgc cgtgttgcag gccgacccgc aggcgcatct cgacatcaca tggagccatc
     3661 ccgaattcgg cgcgatgcac tggcgag
//
LOCUS       DASZAK010000788         4771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_339220, whole genome shotgun sequence.
ACCESSION   DASZAK010000788 DASZAK010000000
VERSION     DASZAK010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339220"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VGT44_13925"
     CDS             complement(<1..812)
                     /locus_tag="VGT44_13925"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2581948.1"
                     /translation="MTDYIVSILTEGAIFGIMALGLNVIWGWAGDFDLAFYGYVALGA
                     YMTLVLTIGTPTPPAEYILGLQLPYLLALPIAVVSSALMGLVVGLIALRHLRGIYFSI
                     VTLGAVYVLYVLAGQYVPLFDGYNGLSGLYNPMGDALGLDFYSYPYFFLGFCLFFFIL
                     VFLFMGRVSGSRFGLALRSLREDERAAAAFGRDIYRLKLKAYVLGAATGGLAGGLFAA
                     YLSAFNPSAWAPIEAIVLYSAVLVGGRGNIKGVILGVVVMILLIQESTRFIP"
     gene            complement(809..1690)
                     /locus_tag="VGT44_13930"
     CDS             complement(809..1690)
                     /locus_tag="VGT44_13930"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2581949.1"
                     /translation="MSEALLILGFGLVTAAILSLSAVAFTLEYAVTRVANLAHGEILT
                     TGAYAAYLVQQATGNAILAALGAAAAGGLLALLMHVAVIDRFVRSGIGTMPTFIATLG
                     MSFVIQNVLVINFGASNVAYTIPQSAPRQVGPFLWTSTEIEILISALVITAVLYVLIT
                     YTKFGKAIRAVFQNRDLARVTGINPRLIASATWLLAGLVAGYAGFILAESVGTFNPYF
                     GFSFFLITLTAAVAGGLGKPFSTMIGAVIVGIALQFTGYYINASYELAFAFAILAAII
                     LVRPRGLFVKAGRAMFE"
     gene            complement(2033..3331)
                     /locus_tag="VGT44_13935"
     CDS             complement(2033..3331)
                     /locus_tag="VGT44_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2581950.1"
                     /translation="MSITKTLSQGKLTGVFKWHGSLRIVSGCLALVLLLAACGSTPTT
                     TTGGGGPVTMGVLSCFTGTLASLGQAMLQGAQVAQRAINEAGGILGRQLNLAHADTQC
                     DEADSVPALRQLLATQNIVGIIGPETQEISADAPIITSAKIPDEFQGGSTLFDHNTNT
                     WLWRDSPSDSQLGVAMALYAHKKGYQKAALLFYSDIAAQTFAAPITATWQKMGHQIVS
                     NVTVAPDQTSYLPQVQQVINAHPDVIFTQTDAATAAVLFQNFKELNNLAIPFVGTDVT
                     GVDQYLKAVTYAAAHAHLTSVYGTSVTGQAADAFNQFFAAQFGSTAQPLANANYAYDA
                     VISLALAIDKANSTSGTAVNAAMTQVTNPPGTQCYTYKDCLNLLNAGKKINYDGASGD
                     LNYNQYQNVFGPYGAFQVNLSGQEQQVMLLSAPDLAAVTP"
     gene            3654..4580
                     /locus_tag="VGT44_13940"
     CDS             3654..4580
                     /locus_tag="VGT44_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEV2581951.1"
                     /translation="MSRFRGAPLLLSCGLLLMLLLSACVDAGSSSPASSSSVLNVIAG
                     ENFWGSIATQLGGTHVSVTSIVSDPNADPHQYETNTSDARTFAQANYVILNGAGYDTW
                     GQKLLDANPVSGRKVLTVSDLLGKHAGDNPHFWYNPSYIEQVSDQITKDYQALDPADS
                     AYFTRQRTAFETALSSYHQRITEIKAKFAGKKVGATESIFVYMANALGLDLISPPAFM
                     GAISEGNDPPASSVVTFEQQVQQKQITVLVYNIQTVTNITNTIKQLAAQQHIPMVGIS
                     ETIQPANASFQDWQTAQLLALQNALNASTVGQ"
BASE COUNT         1106 a         1297 c         1407 g          961 t
ORIGIN      
        1 tcggggatga agcgcgtcga ctcctggatc agcaagatca tgaccaccac gcccaggatg
       61 actcccttga tatttccacg ccctcccaca agaacggcgg agtacagcac gattgcttca
      121 atgggagccc aggctgacgg attaaaggca ctcaggtagg cggcaaagag gccaccggcc
      181 agacctccgg tcgcagctcc gaggacatag gctttcaatt tcagccggta gatgtcacgg
      241 ccaaaggcgg ctgcggcgcg ttcatcttcg cgaagggagc gcagtgcaag accaaatctg
      301 ctcccggaga ctcgtcccat gaagagaaag acgaggatga agaagaacag gcagaagccg
      361 aggaagaaat acggatagct gtaaaagtcg agtcccaggg cgtcccccat cgggttgtac
      421 aggccagaca gcccgttgta accgtcgaac agggggacat actggccggc caggacatac
      481 agaacgtaga ccgctcctaa cgtcacaatg gagaaataga tcccgcgcag atgccggagc
      541 gcgatgaggc ccacaacgag tcccatgagc gctgaggaga caacggcgat aggcaaggcg
      601 agcaggtacg gcagctgcag ccccaggatg tattcggctg gcggggtcgg tgtaccaatc
      661 gtgagaacga gcgtcatgta agcgcccaac gcgacatagc catagaaggc gagatcaaag
      721 tcgccggccc agccccagat gacgttcagg ccaagcgcca tgatcccaaa gatcgcgcct
      781 tcggtgagaa tggagacgat ataatctgtc attcgaacat agccctccct gctttgacaa
      841 agaggccgcg cggacgcacc aggatgatcg cggccagaat cgcaaaagcg aaagcgagtt
      901 cgtagctcgc gttgatgtag tagccggtga actggagagc gatgccgacg atgactgctc
      961 caatcatcgt gctgaacggc ttacccaggc cgcccgcgac cgccgctgtc agggtgatga
     1021 ggaagaagct gaatccgaaa tagggattga acgttcccac gctctcggca aggatgaagc
     1081 cggcatagcc agccaccagg ccggccagca gccaggttgc ggaggctatt agtcgagggt
     1141 tgatgccggt gacgcgggcc aggtctcgat tctgaaatac ggcacggata gctttgccga
     1201 actttgtgta cgtgataagc acgtaaagca cggctgtgat gacaagcgcg gaaataagga
     1261 tctcgatctc ggtggaggtc cataggaatg gtcctacctg gcgcggagcg ctctggggga
     1321 tcgtataggc gacgttagac gcgccgaagt ttatgaccag cacgttctgg atcacgaagg
     1381 acatgccgag cgtggcgata aaggtcggca tggtcccgat gccactacgc acaaagcggt
     1441 cgatcacggc gacgtgcatc aacagagcca gtaaaccacc agcagccgcg gccccaagcg
     1501 cggcgaggat cgcgtttccc gtcgcctgtt gtacgaggta ggcggcgtaa gcacctgtcg
     1561 tcagaatctc gccgtgggca agattggcca cccttgttac ggcgtactcg agggtgaaag
     1621 ccaccgcaga taacgatagg atagcagcag tcaccagccc gaaaccgagg atcaggagtg
     1681 cttcactcat aacgccacct cctcctttcg attgtcatca atcctcctca tattcctttc
     1741 aaaaagttgg ggtgacgaat gtgtagagaa agagcagcat aaagccatca gcagtattat
     1801 ccctttgcgg cggtgaactc gtcaagtgtt tgcacatagt gatgaaagaa agcagtgatg
     1861 gtcgtgtgga cgcaatgatg aacgtgacaa ccaggatcgg tcatcggttg tcaccctgag
     1921 cgcagcgaag ggtctcgctc gatgggcaga gagatgcttc gctgcgctca gcatgacagg
     1981 atcggctttg gttgttaaaa ttcatcatcg catccctacg acggtcgtca ggttacggtg
     2041 tgacggctgc caggtcgggt gctgagagca gcatgacttg ctgctcctgg ccgctgagat
     2101 tgacctggaa cgcgccgtaa ggtccaaaga cgttctggta ctggttgtag ttgaggtcgc
     2161 cactggcgcc atcgtagttg atcttcttgc ctgcattgag cagattcagg cagtccttgt
     2221 acgtgtagca ctgcgtcccc ggcgggttgg ttacctgggt catggcagcg ttgacggcag
     2281 ttcccgacgt agagttcgct ttgtcgatgg caagggcaag gctaatgacc gcgtcgtaag
     2341 cgtagttcgc attagcaagc ggctgagccg tgcttccgaa ctgggctgcg aagaactggt
     2401 tgaaggcgtc ggcagcctgc cctgtgactg aggtcccgta gaccgaggtg agatgagcat
     2461 gggcggcggc gtaggtgacg gccttcagat attggtcaac accggtgaca tcggtgccta
     2521 cgaacggaat agcgaggttg tttagctcct tgaagttctg gaaaagcacg gcggctgttg
     2581 ccgcatccgt ctgcgtgaag atcacgtccg ggtgcgcgtt gatgacctgc tgtacctggg
     2641 ggagatacga ggtctggtcg ggcgctaccg ttacattgga cacgatctgg tggcccatct
     2701 tctgccaggt cgccgtgatc ggcgcggcga aggtctgagc agcgatgtcc gagtagaata
     2761 gcaacgcagc cttttgatat cccttcttgt gggcgtagag tgccatggcg actcctagtt
     2821 gagagtcgga agggctatcg cgccacaacc aggtattggt gttgtggtcg aagagggtgc
     2881 tgccaccctg gaactcatcc gggatcttgg ctgacgtgat aatgggggcg tcggcactga
     2941 tttcctgggt ttcggggccg ataatcccaa cgatattctg ggtagccagg agctggcgca
     3001 gcgccgggac agagtcggct tcgtcgcact gggtgtcagc gtgagccaga ttgagttgcc
     3061 ttccgaggat gcctcccgct tcattgatcg ccctctgcgc cacctgcgcg ccctgtaaca
     3121 tggcctgccc caggctggcc agcgtcccgg tgaagcagga caggacgccc atcgttacag
     3181 gtcctccccc accggtagtg gtggtaggag tcgatccaca tgccgccagc aggagtacaa
     3241 gtgccaggca gccggacacg attcgcaagg acccatgcca cttgaacaca cccgtcaatt
     3301 ttccttgaga cagtgttttt gttatggaca tgtttgttcc ctccatagtc gacttctaaa
     3361 ataggcttgc tctttttctc ttcctttcta ggcttctcct cctttcttag aacaggtcgt
     3421 ttcccacgac aggcaaaacg gcccgatccg tccttagatg aataaagatt tgtcacaagt
     3481 ataggtgata agctataaaa aagccgtaaa tacggccata gaaatataac taaatgcata
     3541 acaattcccc tccctcttaa tgagacaaaa tatcattgac aggtcgctcc tccctatgtt
     3601 atagtaacaa cgaagtattt gatacagtgt ctctttatga ggggggtcgt ctgatgtctc
     3661 gtttccgcgg agcgccattg ctgctttcct gcgggctgct gcttatgcta ttgctgagcg
     3721 cgtgtgttga tgccgggagt tcttcgcccg cgtcgtcatc aagcgttctc aatgtcattg
     3781 ccggtgaaaa cttttggggc agcattgcca cgcagcttgg tggcactcat gtgagcgtga
     3841 cgagcattgt gagcgacccc aatgccgatc cgcatcagta tgagaccaac accagcgacg
     3901 cacggacctt cgcgcaggct aattacgtga tactcaacgg cgcaggatac gatacatggg
     3961 ggcagaagtt actggacgcc aacccggtga gcgggcgcaa ggtattgacc gtctcagacc
     4021 tgttgggcaa acacgcgggc gacaaccccc acttctggta taacccgagc tatatagagc
     4081 aagtgtccga ccagatcaca aaagactacc aggcgctcga tcccgccgat agcgcctatt
     4141 tcacccggca gcgcaccgcc ttcgaaacgg cgctgagcag ctatcaccag cgcattaccg
     4201 aaatcaaggc gaagtttgcc ggaaagaagg tcggggcgac ggagagcatc tttgtttata
     4261 tggccaacgc gctggggctg gacctgatct cgccgcctgc gtttatgggt gctatctccg
     4321 agggcaacga tccgcccgcg agcagcgtgg taacattcga gcagcaagtg cagcagaagc
     4381 agattacggt gctggtctac aacatacaga ccgtcaccaa tatcacaaac acgatcaagc
     4441 aactcgcggc tcagcagcat atcccgatgg taggtatatc ggagaccatc cagccggcca
     4501 atgctagctt ccaggactgg cagacagcgc aattgctggc attgcagaac gcgctgaacg
     4561 ccagtacggt cggccagtaa ggtcactcgt aggcgccggt ttaccgtgcg catcgccgat
     4621 ttatcggccc ggctgcattg tcgaccagag caattggaca ggccgatcat gcagattgac
     4681 aaactactcc ggccaccgga aagacaaggg cgaccgcaag ggtctccacc cggctccgct
     4741 ccacccccgc ccttacaaag aacggcgagt g
//
LOCUS       DASZAK010000789         6720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_20050, whole genome shotgun sequence.
ACCESSION   DASZAK010000789 DASZAK010000000
VERSION     DASZAK010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6720
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20050"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1422
                     /locus_tag="VGT44_13945"
     CDS             145..1422
                     /locus_tag="VGT44_13945"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV2581952.1"
                     /translation="MSEIGTLPPNYFYHRLPNGIELIGQYMPSLSSITFGFQLDAAVI
                     HEPEDKQGLAHLFEYMLFQGTKQKDARALNEAFESLGARKGASTSLETAQVFAQIVNT
                     KFDATLELISEVMRTPTFPRDELEQMRSLVLQEIRRRDDEPMSRIFELVRTNFYQGTA
                     LSRPALGTSESVAQLQRADLRAFWQERYRPNITLFAIAGKFDWDHVVSRVEQLFGDWQ
                     GSATPSPEQHPRPEARVTLEHQDGKQEHLALMFPFPAYTDAEFYAAQVVGEVLGGNMA
                     SRLFVEVREKRGLVYGVSAGLASNKAIGAMRIYAGTTPEQGRECLQVIVNELRKLEQD
                     GITADELERAKVQLKSENVMRGEGSGARMGAISRSWWYERKLRTIQEIKEAIDGVTQE
                     QVLDVLRRYSPLHPLAVAAIGPLSQDELVGDTL"
     gene            complement(1417..2430)
                     /locus_tag="VGT44_13950"
     CDS             complement(1417..2430)
                     /locus_tag="VGT44_13950"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV2581953.1"
                     /translation="MEDFTSLNLALGKASSAPLLYFWAFGDLHYRAQEDWHALHSRRL
                     APMFHDVRTLWLDDGPPAFCVSPGDIVDTGAPQNYTLARNDLAAQLANIPFYPGIGNH
                     EFHRESRQDTIHTAAEYSAAWEKPLFYSWQIGQFDEITCIMLDQPDPYEDNPTRENPK
                     VIFSPATLAFLDTTLEQHSGQVGNIAVIFAHCPLYNTVLDRDPDLNLDDDSLDPFFHV
                     ENSDEVRAILARHAHAAFYISGHTHSGWGSPNLITTEMLGGHPIHPVHPVTHVNLMSP
                     WYTGRHRGPRLSADRLALEYYPDDPDVLISFAIRVYRHHAVIAARDHRAHRWLAQWIA
                     PLL"
     gene            complement(2633..3352)
                     /locus_tag="VGT44_13955"
     CDS             complement(2633..3352)
                     /locus_tag="VGT44_13955"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEV2581954.1"
                     /translation="MHSRDRFNKFTERARKVLQLAQEEAQRLNHNYIGTEHLLLGLVR
                     EGEGVAAKVLTELGVDLEKVRKAVEDIIGRGDRTVYGEVGLTPRAKKVMELAVDEARR
                     LNHHYIGTEHLLLGLLREGEGVGARTLESFGLNLQQVRAKIIQVLNQQSRQPHACSFC
                     GKQQDQVQRLIAGPREVYVCDECVAKLSISADAPAEDQGLRCSFCGKKQNQVQRLAIG
                     PHGVNICNQCIVICQEIIAEE"
     gene            complement(3473..4279)
                     /locus_tag="VGT44_13960"
     CDS             complement(3473..4279)
                     /locus_tag="VGT44_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581955.1"
                     /translation="MKEQNFEKRKQQEPVDVEQRLSAYFGPPLPEQPLSSDSWQKLRY
                     RLAPREDAGRRRCNHLPRRRSQAHVSIALQDAFARIVYRAGVAYPPPKLLCMLKLRLR
                     EPSVRISWLGRRAIRLLLPINAMTSMGQDELDVLLATGLARSITARKPKYMLGRLLLA
                     GVALLACLTLILSWIHHALLIGLPLAVVLCALVVVVWQLQTRSIAFRADTLMVRWLGR
                     SRACSGLHALADRSRTPRRRRWGEPSLVERIQRVCGTRVEARDHELTLVG"
     gene            complement(4276..4848)
                     /locus_tag="VGT44_13965"
     CDS             complement(4276..4848)
                     /locus_tag="VGT44_13965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2581956.1"
                     /translation="MATNEAELIACAAHGDQEAFRQLWEAHHAAAMAAALRLCHQRAL
                     AEEITQGAFLLAWRGLPRFQQGSPFRPWLMRILYRHALDVTEKQRTYLRSVSLDDALE
                     LSAPSSQEGRPEQADPQERVAQREELRQALAQLSSEQRRVIALRYGADLTEMDIAQVL
                     GWPVGTVKSRLNRARARLRELLSDEERRLP"
     gene            4994..6004
                     /locus_tag="VGT44_13970"
     CDS             4994..6004
                     /locus_tag="VGT44_13970"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2581957.1"
                     /translation="MKHCLSHRRSFPLLALGLILLLLSACGTTTSSGTTPTPAAKGTV
                     AVAYAASLSNIMEHAVKSAFDQSTGYAYQGEAKGSSALINEIKGKLSFPDVFISANPK
                     LNAQLMGAANGNYASWYLDFARAEMVIGYNANSKFAADFQAAANGSKQWYQVLQEPGL
                     RLGRTDPLLDPKGVNTIYTLELAEAYYHQPGLTQKILGGDENTSQIFPEEELVARMGA
                     GQLDAGFFYINEVKGPSIPYISLPDQVNLGNATMATQYATVSWKNPKTNAVSKGAPVV
                     YTITVLNTAKNKAGGIAFANFLLSSQGQSLLQAQGVLTTPFTAGGDTTTIPQQLQRYV
                     QS"
     gene            6026..>6720
                     /locus_tag="VGT44_13975"
     CDS             6026..>6720
                     /locus_tag="VGT44_13975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2581958.1"
                     /translation="MQRLIITDLDQRRTSPFSLAGKGIIFWLLGGCMMLYLVGPLVYF
                     LFVLPWSAAPSTLSDPDAIQALTTSAISATIATACMALFGIPLGYVLARFSFPGKSIV
                     SVAIYLPLVFPPVVSGIILLVLFGPYGLIGGPLANAGWEVDDTLAGIVIAQIFVAAPF
                     VIVAARSAFESIDPRLEQVAATLGHSRWRLFWRVSLPLARGGIIAGLVLAWMRALGEF
                     GATVVLAYHPYTIP"
BASE COUNT         1350 a         1874 c         2068 g         1428 t
ORIGIN      
        1 cgttatcgta gtggcgaccc ttgcggtcgc catgccgccg cgccatgccc ctactcctcc
       61 cttgcgtgtg atatactggt catgttctct tcgagttgcc cacgatacat tatccattac
      121 gttcagaaag gaacgtttgc atatatgagc gagatcggga cgttaccgcc aaactatttt
      181 tatcatcgtt tgccgaatgg cattgagttg attgggcaat acatgcccag tttgagttcg
      241 attacgtttg gcttccagct tgacgcggct gtgattcatg agccggagga caagcagggg
      301 ctggcgcacc tgttcgagta catgctgttc caggggacga agcagaagga tgcgcgggcc
      361 ttgaacgaag ccttcgagtc gctgggggcg cgtaaagggg ccagcacgag cctggagacg
      421 gcgcaggtct tcgcgcagat cgttaatacg aagttcgatg cgacgctcga actgattagc
      481 gaggtgatgc gcacgccgac attcccgcga gacgagctag agcagatgcg cagcctggta
      541 ctccaggaga ttcgccgccg cgacgatgag ccgatgagcc gtatcttcga gctggttcgc
      601 acgaatttct accaggggac ggcgttgagc cgcccggcgc ttggcacatc cgagagcgtg
      661 gcgcaattgc agcgagcgga cctgcgcgcg ttctggcagg agcgctatcg cccaaacatc
      721 acgctattcg ccatcgcggg caagttcgat tgggatcacg tggtgagccg ggtcgagcag
      781 ctcttcggcg actggcaggg cagcgcgacg ccctcgccgg agcagcatcc gaggccggag
      841 gcgcgcgtca cgctcgaaca ccaggatggg aagcaagaac atctggcgtt gatgttccct
      901 tttcccgcct acaccgacgc ggagttctat gcagcccagg ttgtcggcga agtgctgggc
      961 ggcaatatgg cgtcgcgcct cttcgtcgag gtgcgcgaga aacgcggcct ggtctacggc
     1021 gtctccgcag ggctggcgag caacaaagct atcggggcaa tgcgcatcta tgcggggacg
     1081 acgccggaac agggccgcga atgcctgcaa gtgatcgtca acgagctgcg caagcttgag
     1141 caagatggca ttacggcaga tgaacttgag cgcgctaagg tgcagctcaa gagcgagaac
     1201 gtgatgcgcg gggagggttc gggcgcgcgt atgggcgcga ttagccgctc ctggtggtac
     1261 gaacgcaagc tgcgcacgat ccaggaaatc aaagaagcca tcgatggcgt cacgcaggag
     1321 caggtgttgg acgtgctgcg ccgctattcg ccgctgcatc ccctggcggt cgcggccatc
     1381 gggccgttgt cgcaggatga actggtgggg gatacactat agtagagggg ctatccactg
     1441 tgccagccag cggtgggcgc gatggtcgcg ggcggcgatg acggcgtggt gacgatagac
     1501 gcggatggcg aacgagatca gcacgtcggg atcgtcgggg taatattcga gcgccaggcg
     1561 gtcggcgctg aggcgcgggc cgcgatgcct gccggtgtac cagggactca tgaggttgac
     1621 gtgggtgaca ggatggacag gatggatggg atggccgccc agcatctccg ttgtaatcag
     1681 attgggcgaa ccccagcccg aatgggtgtg accgctgatg tagaacgcgg cgtgggcatg
     1741 gcgggccagg atagcgcgca cctcgtccga attctcaacg tgaaaaaaag gatcgagcga
     1801 atcgtcgtcg agattgagat cggggtcgcg gtccaggacg gtattataca gggggcagtg
     1861 agcgaagatg acggcgatat tgccgacctg cccggagtgc tgttccagcg tcgtgtccag
     1921 gaaggccagc gttgcgggcg agaagatcac tttgggattt tcgcgcgtgg ggttgtcctc
     1981 gtatgggtcg ggttgatcga gcatgatgca ggtgatctcg tcaaattgtc cgatctgcca
     2041 gctatagaac agcggcttct cccaggcggc gctatactct gcggcagtgt ggatcgtatc
     2101 ctggcggctc tcgcgatgga actcgtggtt gccgatgccg ggataaaagg ggatattggc
     2161 gagctgcgca gcgaggtcgt tgcgggcgag ggtgtagttc tgcggcgcgc cggtatcgac
     2221 gatatcgccg ggcgaaacgc agaaggcggg cggaccatcg tcgagccaca atgtgcgcac
     2281 atcatgaaac atgggggcca gccgccgcga atgcagcgcg tgccagtctt cctgcgcgcg
     2341 ataatgcagg tcgccaaatg cccagaagta gagcagcggg gcgctgctgg ctttgcccag
     2401 ggcgaggtta agcgatgtga aatcttccat gtgtgcgggt ttgctccttg tatcatttta
     2461 tgaattttac cgatcaagtc tggtgctgtc atgctgagcg cagcgaagca tctcgcaacc
     2521 catccaccga gacccttcgc tgcgctcagg gtgacaatca atgacctgac ttgattgaga
     2581 aaaatcatta atgatattgt agcgcacgtg ggttaaaggg atgtaaagca ggttactctt
     2641 cagcgatgat ctcctggcaa atcacgatgc actgattaca gatgtttacc ccgtgcgggc
     2701 cgatggccag gcgctgcacc tggttctgtt ttttcccgca gaatgagcag cgcagccctt
     2761 gatcctcggc gggcgcatca gcgctgatgc ttaatttcgc gacgcattcg tcgcagacgt
     2821 agacttctct gggtccggcg atgaggcgct ggacctggtc ttgctgcttg ccacagaagg
     2881 aacaggcatg tggctgcctg ctctgttggt tgagtacctg aatgattttt gctcgcacct
     2941 gctgcaagtt gaggccaaag ctttccagcg ttctggcacc gacgccttca ccttcacgga
     3001 gcaagcctaa cagcaggtgt tcagttccta tgtagtgatg attgaggcgt cgcgcttcat
     3061 caacggccaa ctccatcact tttttggcgc gcggggtcag gccgacttcg ccgtacacgg
     3121 tgcggtctcc acggccaatg atgtcttcga ctgccttgcg caccttctca agatcgacac
     3181 caagctccgt gagtactttg gcggcaacac cttcaccctc acgaacaagg ccaagcaaga
     3241 gatgttctgt gccgatgtag ttgtggttga ggcgctgcgc ttcttcctgg gcaagctgca
     3301 agactttcct ggctcgctcg gtgaacttgt taaacctgtc tcgactgtgc atttgtgcgt
     3361 tactcctctt tccacggaga ccaactctcg gagaaatcca acatattaat ctgtggatgg
     3421 agcgatttaa tccatatcct gacatcttct gatcctttcg ggtttcacca gattaaccga
     3481 caagcgtcaa ttcgtgatct ctagcctcca cgcgtgtccc acatactcgc tgaatgcgtt
     3541 ctacaaggga tggttcgccc catcgcctgc gccggggtgt ccggctgcga tctgccagcg
     3601 cgtgcaatcc gctacaggcg cggctacggc ctaaccagcg caccatcaaa gtgtccgcgc
     3661 ggaaggcgat ggagcgtgtt tgcagttgcc agacgacgac gaccagggcg caaagaacga
     3721 cagcgagagg taatccaatc agaagcgcat ggtgtatcca gctcaagatg agcgtaagac
     3781 atgccagcaa cgccacacca gcgagcagga ggcggccaag catatatttt ggtttgcggg
     3841 cggtgataga acgcgccagt cccgtcgcca gcaacacatc gagttcatcc tgacccatgc
     3901 tcgtcatggc gttgattggc aggaggagcc gaatggcgcg cctgcccaac cacgaaatgc
     3961 gcacagatgg ctcgcgaagg cgcaatttca acatgcagag gagctttggg ggaggataag
     4021 ccacaccggc tctgtagaca atgcgagcaa aggcatcttg cagagcgatg gatacatgcg
     4081 cttgcgatct tctgcgtgga aggtggttgc agcgtctgcg ccccgcgtct tctcgcggtg
     4141 caagtcgata gcgcagcttc tgccacgagt ctgatgataa cggttgttct ggaagcggcg
     4201 ggccaaagta ggcgctcaga cgctgctcta catcgaccgg ctcctgctgc ttgcgtttct
     4261 cgaagttctg ttctttcatg gcaacctcct ctcttcatcg ctcaacagtt cgcgcagacg
     4321 cgcccgcgcg cgattgagtc ttgatttgac cgtgcctacc ggccagccca acacctgcgc
     4381 aatgtccatt tctgtgagat cagcaccata gcgcaaggca atcacgcgcc tctgctcgga
     4441 actcaactgc gccaacgcct gtcgcaactc ttcacgctgg gctacgcgct cctgcggatc
     4501 ggcctgttct gggcgaccct cctggctgct tggcgctgat agctcaagag catcgtccag
     4561 ggaaacagag cgcaggtacg tccgttgttt ctccgtcaca tccagcgcgt gtcgatagag
     4621 gatgcgcatc aaccaggggc ggaacggact gccctgctgg aaacgtggaa ggccacgcca
     4681 ggccaggaga aacgctccct gcgtgatctc ttccgccagc gctcgctggt ggcagagtcg
     4741 caacgcagca gccatcgcgg cggcatgatg agcttcccag agttgccgaa aagcttcctg
     4801 gtcgccatga gccgcgcacg cgatgagttc agcctcattg gtagccacgc atttcctcct
     4861 tctgtagcaa tagtacaccc aaaggagggg aaaaggttcc ggggtaactt cgccttgacc
     4921 agcagccggt tgccgcttat aatcatacta tgagtactca tttgtagagt atattgccag
     4981 gaggaaccat cgcatgaagc attgcctttc ccatcggcgt tcattcccat tgctcgcgct
     5041 cggccttatt ttgctgctgc tcagcgcgtg tgggacgaca acatccagcg gcactacccc
     5101 aacgcctgcg gcaaagggga cggttgcggt cgcctatgca gcctcgctgt ctaacatcat
     5161 ggaacatgcg gtgaagtctg cattcgatca gagtacgggc tacgcatacc agggtgaagc
     5221 gaaaggatca tcagcgctca tcaatgagat caagggcaaa ctgagcttcc cggatgtctt
     5281 tatcagcgct aacccaaagc tcaatgcgca gttgatggga gcggccaacg ggaattatgc
     5341 gtcctggtac ctggactttg ccagggctga gatggtgatc ggctataatg caaacagcaa
     5401 gttcgccgcc gatttccagg cagccgccaa tggcagcaag cagtggtacc aggtgttgca
     5461 ggagcctgga ttgcgcctgg gccgtactga tccgctgctt gaccccaaag gggtgaacac
     5521 gatctacacg cttgaactgg cggaggcata ctatcaccag cctggtttaa cgcagaaaat
     5581 cctggggggc gacgagaaca ccagccagat tttccctgaa gaggaactgg tcgcgcgcat
     5641 gggcgcgggc cagctcgatg ccggtttctt ctacatcaac gaggtcaagg ggccgagcat
     5701 tccttacatt tcgctgcccg accaggtcaa cctgggcaat gcgacgatgg caactcaata
     5761 tgctaccgtc tcgtggaaga atcctaaaac gaacgcggta tccaaaggcg ctcccgtcgt
     5821 gtatactatt actgtcctca ataccgcgaa aaataaggcc gggggcatcg ccttcgccaa
     5881 tttcctgctc tcgtcccagg gtcaatcctt gctccaggcg cagggtgtgc tgacgacgcc
     5941 ctttacggcg ggtggggata cgacgaccat acctcagcaa ttgcagcgat acgtgcagag
     6001 ctaacgaaag gatagtccgg cattcatgca gcgtctcatc attacggacc tcgatcagcg
     6061 caggacttcg cctttcagcc tggcagggaa ggggattatc ttctggctgc tgggcggttg
     6121 catgatgctg tacctggttg gcccgctggt ctacttcctg ttcgtactgc cctggtcggc
     6181 ggcgccgtca accttgagcg acccggacgc gatccaggcg ctcaccacct cggcgatcag
     6241 cgccacgatt gcgacggcgt gtatggcgct gttcggcatc ccgcttggct acgtgctggc
     6301 gcgttttagc tttcccggca agagtatcgt gagcgtcgcg atttacttgc cgctggtgtt
     6361 tccgccggtt gtcagtggta tcattctgct ggtgttgttt ggtccttacg gcctgatcgg
     6421 cggcccgctg gcgaatgccg gttgggaagt cgatgataca ctggcgggca tcgtgatcgc
     6481 gcaaatattt gtggccgcgc cctttgtgat cgtggcggcg cgctcggctt tcgagtcgat
     6541 tgatccgcgc cttgagcagg tagccgcgac gctgggacac agccgctgga ggcttttctg
     6601 gcgtgtgagc ctgcccctgg cgcgcggcgg catcatcgct ggactggtgc tggcctggat
     6661 gcgcgcgctg ggcgagtttg gcgcgacggt ggtgctggcc taccatccgt ataccatacc
//
LOCUS       DASZAK010000790         7658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_232735, whole genome shotgun sequence.
ACCESSION   DASZAK010000790 DASZAK010000000
VERSION     DASZAK010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232735"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VGT44_13980"
     CDS             complement(<1..597)
                     /locus_tag="VGT44_13980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2581959.1"
                     /translation="MESFKKVLMLVENQPAPADRRVWPEALALRDMGFQVTIISPKGS
                     EKHRESHVCLEGIHIYRYQLPQAGNKYMAYFAEYGVALLMTYLLSLKVLFRHGFDVIH
                     AANPPDIFFLIGLFYRLLGKKYVFDQHDLSPEMFQVIYKGRMKPLHSILLFLERCSYR
                     TAHVVIASNASQGRFARARGGCRKVFVVRNGPDLKSLQM"
     gene            complement(622..1854)
                     /locus_tag="VGT44_13985"
     CDS             complement(622..1854)
                     /locus_tag="VGT44_13985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581960.1"
                     /translation="MVSEAPLLVKGQVNTSVPVVVIAPGYHGHAIARSLGRLGVSVYG
                     VHADRRSPAARSRYWKENFFWNIAKAAPEKTVERLLQLGQQIGARPLLIPTDDDSCVF
                     AADHADSLREAFLFPEQPAGLARSLSSKKQMYYLCKKFGIPAAETSFPQSREDVIEFG
                     KHAAFPIMLKGIDTLALKRRTGVKMVVAQDAATLLKLYDEMETPESPNLMLQEYLPGT
                     QRVWMFDGYFNAESECLFGLTANMVRQYPAYTGVTSLGVCYYNDIVAMQTKKFMAALG
                     YRGPLDIGYKYDERVGQYKAIDVNPRIGMTFRLLVDSAGMDVARAFYLDMTGQPVPAG
                     EPREGRKWMVENFDLVSSPRYVRDGKLGIRGWMRSYRGVEETSWFAPDDPLPFFSMGW
                     RSLRWAIDKRFKKTGATL"
     gene            complement(1877..2605)
                     /locus_tag="VGT44_13990"
     CDS             complement(1877..2605)
                     /locus_tag="VGT44_13990"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0050532 - 2-phosphosulfolactate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEV2581961.1"
                     /translation="MRKTVVIDCFPESVENYRSGYALVAVDVIRATTTAVACVASGRK
                     CFPVPSLEAAVPLAAKLPNPLLVGELGGSMPYGFDLNNSPADIEARTDIHRPMLLLST
                     SGTKLMCGAAGSQAMYVACLRNYRATIAHLAAHHPRVAVIGAGSRGDFREEDQMCCAW
                     IADGLLAAGYEAEQGQTSAIIERWRGAPVDAFVDGKSAEYLRNSGQVRDLDYILEHVD
                     DLDMVYRYEGGQVVKHAEEFLALS"
     gene            complement(2623..3441)
                     /locus_tag="VGT44_13995"
     CDS             complement(2623..3441)
                     /locus_tag="VGT44_13995"
                     /EC_number="4.4.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEV2581962.1"
                     /translation="MGEIQRRLSTHDYLRLIGVPDLPPRTSPFDPGYDPVTLVSHIEQ
                     SAHLMASLKISMACWQIANEAATRRKIAAARAHHVPVVTGGGPFEVAVAQKQLEAYLD
                     LCAGLGITRIESGEGFTDLPHKPRVIVQMAHERGLEVQYEMGKKHEGPFTKESLEDAI
                     ARGHAWMDAGAVQVVVEARESAKGVGMFGDDGTLNTGYADRFANEFGLEVALFEAPNK
                     PSQFAFMEHFGPYVHLCNVRIEELLRVEIFRRGLHSDAFAMPNLRPASPSDVTI"
     gene            complement(3462..4373)
                     /locus_tag="VGT44_14000"
     CDS             complement(3462..4373)
                     /locus_tag="VGT44_14000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2581963.1"
                     /translation="MKKSADMRLEGQANVNAYFQTQSSFWNDIYSSSDVYAEIHQQRH
                     AAILEWIDRLGLPPGSQALEIGCGAGFMSIELARRGFHVQAIDSVQAMVELTRSHALE
                     AGVEEHLSVEVGDITALAYEQDAFDLTLAIGVIPWIERVGPAMQELARVTRPGGYVIL
                     TADNRARLNNLLDPWLNPMFAPLKRAIKRMLMRAGLRRSSLKDVGATGHSRRFIDASL
                     ARADLRKNSSKTLGFGPFTLLRHAVLPKSLGVRLHHRLQQLADRGAPILRSTGAQYIV
                     LAEKPMSSRRTQSIGAGSAASGDTQAL"
     gene            complement(4546..5583)
                     /locus_tag="VGT44_14005"
     CDS             complement(4546..5583)
                     /locus_tag="VGT44_14005"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEV2581964.1"
                     /translation="MSEHNSPDRVVLITAPLEAGWLERLRCISPDLRVEWIPHGTGVI
                     SDELWQNAEILYTSFATRLPPPEMAPRLRWAQLYSAGADRVVDTALYRSPVVFTSASG
                     VHAINIAEQVLSMTLAWFRHVPLILDQQRQHRWPANAGERARLYMAEELWGKTIGIVG
                     YGSIGRQVAALARAFGMRVLAMQRGEDHRDRGFLFPGVGDPEGLLPERYFAPEQLHAM
                     LRECDVVVIALPLTTRTRGLFNAAAFQAMPSTAFLVNIARGEICDEAALLHALETQQI
                     AGAALDVFREEPLPPHHPFWQLPNIFITPHSAGLTPRYNERAAMIFEENLRCFLAGRP
                     LYNVVDKTLEY"
     gene            complement(5580..6623)
                     /locus_tag="VGT44_14010"
     CDS             complement(5580..6623)
                     /locus_tag="VGT44_14010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEV2581965.1"
                     /translation="MELVNVFDYERLAQARIEPGAWDYYMSGSDDEVTLRENRAAFER
                     IRLRPRMLVDVSACDTRTTVLGAPVAMPILVAPTAYHCLAHPDGECETARGVSSAGTL
                     MVASTSATRSLEEIAQAASGLRWFQLYIHDRIVTEQLVRRAEAAGYSALVITVDSPRW
                     GHKERPLHSDFRLPAHLRKANFPDPAIPQNFVFVTWESLDWLRSLTALPILLKGILTA
                     EDADLAVERGVEGIIVSNHGGRQLDSVAASIEALPEVVEVVRGRCEVYMDGGIRRGTD
                     ILKALALGARAVLVGRPVLCGLAVDGANGVRQVLEILRAELELAMRLAGRPTLASIDR
                     ALVQLPGERRPVS"
     gene            6781..7620
                     /locus_tag="VGT44_14015"
     CDS             6781..7620
                     /locus_tag="VGT44_14015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2581966.1"
                     /translation="MNEAMPSELRIGRYGRLQAKLFQDMLRELGVEMHPDSLMLDFGC
                     GEGGIVYQLRKQGLKAFGVDIVNDYAWVQQRCLDEGLAQEGEDTFRLIDVENYRIPFD
                     DNTFDVIFSDQVFEHVQNYPQALSEIKRVLKPGGSSLHIIPSRYRPVEGHLFIPLGGI
                     FQGRRYLAFWARLGIRNSFQQGQRRQEVADFNYDYLKHSTTYYTKSKIKRLFTAAFGN
                     VTFVEPVFIHCHFGRVRRYLYPITRRFPAMAALFCTLHSRVVFSTKAVAEWPANAAVR
                     REQ"
BASE COUNT         1562 a         2322 c         2246 g         1528 t
ORIGIN      
        1 catttgcagg ctcttgagat ccggcccatt gcgcacgacg aagaccttgc ggcaaccgcc
       61 gcgcgcgcgc gcgaagcgcc cctgcgatgc attcgatgcg atgacgacgt gggctgttcg
      121 atacgagcag cgttccagaa acagcaggat gctatgcagc ggcttcatgc ggcctttgta
      181 gattacctga aacatttccg gggagaggtc gtgctgatcg aacacatatt tcttgcccaa
      241 tagccggtag aacaggccaa tcagaaagaa gatatctggc ggattggcgg cgtgaataac
      301 gtcgaagccg tgacgaaaca acactttcag gctcaacagg taggtcatga gcagcgcgac
      361 gccatattca gcgaaatagg ccatatattt attgcccgcc tggggcagtt gatagcgata
      421 gatgtgaatg ccttcaagac agacatgcga ctcccggtgc ttttcgctgc ccttggggct
      481 gatgatcgtg acctgaaatc ccatatcgcg cagcgccagg gcttccggcc agacgcggcg
      541 gtcggcggga gcgggttgat tttccaccag catcaggact tttttgaagc tttccatgcc
      601 tgtctctcct tcacaagatg gttacagagt cgccccggtt tttttgaagc gtttgtcaat
      661 cgcccagcgc agcgagcgcc atcccatcga gaagaaaggc agcgggtcat cgggagcgaa
      721 ccacgaggtt tcctcaacgc cccggtagga acgcatccac ccgcgaatgc ccagcttgcc
      781 atcgcgcacg tagcggggtg atgagaccag gtcgaaattc tcaaccatcc atttgcggcc
      841 ttcgcgcggc tctcccgccg gcactggctg gccggtcatg tcgagataga atgcccgcgc
      901 cacgtccatc ccggcgctat cgaccagcag gcggaaggtc atgccgatgc gcggattaac
      961 atcgatggcc ttatactgcc cgacccgctc atcgtacttg tagccaatat ccaggggacc
     1021 ccggtagcca agcgccgcca taaatttctt ggtctgcatc gccacaatgt cgttgtagta
     1081 gcacacaccg aggctcgtca cgccggtgta agcgggatac tggcgcacca tattcgccgt
     1141 gagaccaaac aggcactccg attccgcgtt aaagtagccg tcgaacatcc agactctttg
     1201 tgtgcctggc aggtactcct ggagcatcag attgggcgat tccggcgtct ccatctcatc
     1261 gtacagcttg agtagggtcg cggcatcctg ggccacgacc atcttcaccc ccgtgcgccg
     1321 cttcagcgcc agggtatcga tgcctttgag catgatgggg aaggccgcgt gcttgccgaa
     1381 ctcgatcaca tcttcgcggc tctggggaaa gctcgtctcg gccgccggga tgccaaactt
     1441 cttgcatagg tagtacatct gcttcttgct cgaaagcgag cgagcgaggc ccgcaggctg
     1501 ctcagggaag aggaaggctt cccgcaagct gtcggcgtgg tcagcagcga aaacgcagct
     1561 gtcgtcatcg gtaggaatga ggagggggcg cgcgccgatc tgctggccga gttgcagcaa
     1621 gcgctcgacc gttttttcgg gggcagcctt cgcgatattc cagaagaagt tctctttcca
     1681 atagcgcgag cgcgccgccg gggaacgccg gtcggcatgg acgccgtaca cagacacgcc
     1741 gagccgaccc aggctgcgcg caatagcatg gccgtgatag cccggcgcaa tcacgacgac
     1801 gggtaccgac gtgttcacct ggcccttgac cagtagaggc gcttcagaga ccatatcagc
     1861 attcctgcgc tttctctcaa ctgagtgcta aaaattcctc ggcgtgtttg acgacctggc
     1921 cgccctcgta tcgatacacc atgtcaagat catccacgtg ttccaggata tagtccaggt
     1981 ctctgacctg gccgctgttg cgaagatact cggcactttt cccatcgacg aaggcatcga
     2041 cgggcgcgcc ccgccaacgc tcgatgatgg cagacgtttg cccttgctcc gcctcgtacc
     2101 cggcggcgag caggccatcg gcgatccagg cgcagcacat ctgatcctct tcgcgaaaat
     2161 caccgcgcga acccgcgccg atcaccgcaa cccgtggatg atgcgccgcc aggtgcgcga
     2221 tggtcgcccg gtaattgcgc aggcaggcca cgtacatcgc ctgcgatccg gcggccccgc
     2281 acatcagttt cgtgccagag gtcgagagca acagcatggg gcggtggata tccgtcctgg
     2341 cctcgatgtc agccgggctg ttgttcaggt cgaaaccgta gggcatcgat ccgcccagtt
     2401 cccccacgag cagcgggttt ggcagcttcg cggcgagcgg cactgcagcc tcgagcgagg
     2461 gaaccggaaa gcacttgcgc ccggaggcaa cgcacgccac ggcggtcgtc gtcgctctga
     2521 tgacatccac cgctacaagc gcatagccgc tgcggtagtt ctcgacgctt tcgggaaagc
     2581 aatcaatcac cactgtcttt cgcacaacat actccttttg tcttagatcg ttacgtctga
     2641 agggctggcc gggcgcaaat ttggcatggc gaaggcatcc gaatgcaggc cgcgccggaa
     2701 gatttcgaca cgcagcagct cttcgatgcg cacgttgcag agatggacgt atgggccgaa
     2761 atgctccata aaggcgaact ggctgggctt gttgggcgct tcaaacaggg caacctccag
     2821 cccgaattca ttggcaaagc gatcggcgta accggtgttt aaggttccat cgtcgccgaa
     2881 catacccacg ccctttgcgc tctcgcgcgc ctcgacgacg acctgcaccg cgccggcgtc
     2941 catccaggcg tggccgcgcg cgatggcgtc ctccagcgac tccttggtaa agggcccttc
     3001 gtgtttcttg cccatttcgt actgcacttc gagtcctcgc tcatgcgcca tctggacaat
     3061 cacgcgcggt ttgtgcggca gatcggtgaa gccctcgccg ctttcgatgc gggtgatgcc
     3121 gagtcccgcg cagagatcga ggtacgcctc gagctgcttc tgggcgaccg ccacctcaaa
     3181 gggaccgccg cctgtgacaa ccggcacatg atgcgcgcgc gccgccgcga tcttgcggcg
     3241 tgtcgccgcc tcgttggcaa tctgccagca cgccatcgag atcttcagcg aggccattag
     3301 atgcgcgctc tgctcgatat ggctcacaag cgtgacggga tcgtagcccg gatcaaaggg
     3361 actggtgcgc ggtggcaggt ctggaacccc gatcaatcgc aaatagtcgt gggtactcag
     3421 tcgtcgctgt atttctccca tagctctcct ccatttcttg ctcaaagcgc ctgcgtatcg
     3481 ccagatgcag cgctccccgc gccgatcgat tgggtacggc gcgacgacat aggcttttcg
     3541 gccagaacaa tgtactgcgc ccccgtggaa cgcaaaatag gcgcgcctcg atcagcaagc
     3601 tgctgtagtc ggtgatgaag tctaacaccc aaactcttcg gcagcacggc atggcgcaac
     3661 agggtgaacg gtccaaatcc gagcgtctta ctgctatttt tgcgcaggtc ggcgcgcgcc
     3721 agcgatgcgt cgataaaacg gcgactgtgg ccggtcgcgc caacgtcttt cagggatgag
     3781 cgccgcaagc ctgcgcgcat aagcatgcgc tttatcgctc gtttgagcgg ggcaaacatg
     3841 ggattcaacc agggatcgag caggttgttc aaacgcgcac ggttgtcagc tgtcaggatc
     3901 acatacccgc ccggcctggt tacgcgagcc agttcttgca tggccggtcc cacccgctcg
     3961 atccagggga taacgccgat ggcaagtgtc agatcaaagg cgtcttgctc gtaagccagc
     4021 gcagtgatat cacctacctc gacgcttaaa tgctcctcca cgccggcctc cagcgcatgg
     4081 ctgcgcgtca gctcaaccat ggcctggacg gagtcgatgg cctgcacgtg aaaaccacgc
     4141 ctggcaagct cgatggacat gaagcccgct ccacagccga tctccagcgc ctggctgcct
     4201 ggcggcaggc ccagcctgtc aatccattcc agaattgccg catgccgttg ctggtgaatc
     4261 tcagcataaa catcactgct tgagtagatg tcgttccaga acgatgactg cgtctggaag
     4321 tacgcattga cgtttgcctg cccttccagg cgcatatcgg cggacttctt cacgttcggc
     4381 gctccccaaa tagcttctcg gatcacagat gatcccacag ttagcatagc aaggagcggt
     4441 ttagaacggg ttacggccag gggtacatat caggtgagta tggagtaatt gtgtggagaa
     4501 tctggaggat gcttaaaacc ttcgagaact cgctaacagc ctctctcaat actccagcgt
     4561 tttgtctacg acgttataga gcgggcgacc ggccaggaag cagcgcaggt tttcctcgaa
     4621 tatcatggca gccctctcgt tataacgcgg cgtgagtccg gcgctgtgcg gagtgataaa
     4681 gatgttggga agctgccaga atggatggtg tggcggcaaa ggctcttcgc ggaagacatc
     4741 cagcgcggct ccggcgattt gctgcgtttc gagggcgtgg agcagagccg cttcatcgca
     4801 gatttcgccg cgagcgatat tcaccaggaa cgccgtcgat ggcatagcct ggaatgcggc
     4861 ggcattaaac aatcccctgg ttcgtgtagt gagtggcagg gcgatcacga ccacatcgca
     4921 ttcacgcagc atggcatgca gttgttcagg cgcaaaatag cgttcgggga gtagtccttc
     4981 gggatcgccg acgccgggaa acaggaagcc gcgatcgcgg tgatcctccc cgcgctgcat
     5041 ggccagcacg cgcattccga aagccctggc cagcgccgca acctggcgac cgatgctgcc
     5101 atagccgacg atgccgatgg ttttccccca cagctcctcg gccatataca gccgggcgcg
     5161 ttccccagcg tttgcgggcc agcgatgctg ccgttgttga tccaggatca gcggcacgtg
     5221 acggaaccag gccagcgtca tgctcaacac ctgctcggcg atgttgatgg catggacgcc
     5281 gctcgcggag gtgaagacca ccggcgagcg atacagcgcg gtatcgacaa cgcggtctgc
     5341 gcctgccgag tataactgcg cccagcgcaa ccggggagcc atctcaggag gaggaagcct
     5401 ggtggcaaac gaggtataga ggatttcagc gttctgccac agttcgtcag agatcacgcc
     5461 ggtgccgtgc ggtatccact cgacccgcag gtcgggcgag atacagcgca gccgctcgag
     5521 ccatcctgct tcgagcggag cggtgatgag aacgacgcga tcaggactgt tatgctcgct
     5581 caagagacag gccgcctttc tccgggtaat tgcacaagcg cacgatcgat gctcgccaat
     5641 gtgggtcggc ccgcgagccg catggccagc tcgagttccg cgcgcagtat ctccaggacc
     5701 tggcgcaccc cattcgcgcc gtcaacggcc agcccacaga gaacaggtct cccaacgaga
     5761 acagcccgcg cgcccagggc cagagccttc aaaatatccg tcccgcggcg tattcccccg
     5821 tccatgtaga cttcgcagcg tccgcgcaca acctccacaa cctcaggcag tgcctcgatg
     5881 gaggctgcta cgctatcgag ttggcgaccg ccatgatttg agacgatgat tccttccacg
     5941 ccgcgctcca ccgccagatc cgcatcttcc gccgtgagaa tacctttgag caagatcggc
     6001 agggccgtca gcgatcgcag ccagtccagc gattcccagg tgacgaagac aaagttctgc
     6061 gggatcgccg ggtcagggaa gttggcttta cgcaggtggg ccggcagccg aaaatcgctg
     6121 tgaagcgggc gttctttgtg tccccagcgc ggcgaatcca ccgtgataac cagcgcgcta
     6181 tacccggctg cctccgcgcg cctgaccagt tgctccgtca cgatgcggtc atggatgtag
     6241 agttggaacc agcgcagacc gcttgccgcc tgcgcgatct cctccaggct gcgcgtggcg
     6301 gaggtgctgg cgaccatcag cgtcccggcg ctgctcacgc cccgcgccgt ctcgcactcg
     6361 ccgtcgggat gggcgagaca atggtaggcg gtcggcgcga caaggatagg catggcgacg
     6421 ggcgcgccga ggacggtggt gcgcgtgtcg caggcgctga catccaccag catacgcggg
     6481 cgcaagcgaa tgcgctcgaa tgccgcgcgg ttctcgcgca gcgtcacctc gtcgtcgctc
     6541 ccgctcatgt aataatccca cgcgcctggc tctatccgcg cctgcgccag tctctcatag
     6601 tcgaaaacat tgaccagttc catgaaggct ccctgactaa tatggacaac tctgttttgc
     6661 caacatcata ccttaccgtc aaccagatca caacctgcct gttcaatctg gcttgtttac
     6721 ttgtatgcta gaatagtttc tggggcgctg agcaaaatgg agtggaaagg aagcaaggcg
     6781 atgaacgaag cgatgccttc ggagttgcgc ataggtcgct atggacgctt gcaggcgaag
     6841 ctcttccagg atatgttgag ggaattgggg gtggagatgc accccgattc gctcatgctt
     6901 gatttcggct gtggcgaggg cggtatcgtc taccaattga gaaaacaggg actcaaagcc
     6961 ttcggggtcg atatcgtcaa cgattacgcc tgggttcagc agcgctgcct cgacgagggg
     7021 ctggcgcagg agggcgaaga cacgtttcgc ctgatcgatg tggagaacta tcgcattccg
     7081 ttcgatgaca acaccttcga tgtcattttc agcgaccagg tgtttgaaca tgtccaaaac
     7141 tacccacagg ccctatcgga aattaagcgt gtcctcaagc caggcggcag cagtctgcac
     7201 atcatcccct ctcgatatag accggtggaa ggacatctat ttatcccgct tggagggata
     7261 tttcaaggac gccgttacct ggcgttctgg gcgcggctag gcatccgcaa ttccttccag
     7321 caagggcaac gcaggcagga agtggccgac ttcaattatg actatctgaa gcactcaacg
     7381 acctactaca caaagtcaaa aatcaagagg ctcttcaccg cagcgtttgg taatgtgaca
     7441 tttgtcgagc cggtgttcat tcactgccat tttgggcgcg tccggcgcta tctctatccg
     7501 atcacgcgca ggttcccggc catggctgcg cttttctgta cgctccattc gcgggtagtg
     7561 ttttctacaa aggcggtcgc ggaatggcct gcaaacgctg ccgtcaggcg cgagcaataa
     7621 gcacctggcg ctcgtgtttc ttgatgacct gctcgtac
//
LOCUS       DASZAK010000791         1611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_411489, whole genome shotgun sequence.
ACCESSION   DASZAK010000791 DASZAK010000000
VERSION     DASZAK010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411489"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..1213)
                     /locus_tag="VGT44_14020"
     CDS             complement(86..1213)
                     /locus_tag="VGT44_14020"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2581967.1"
                     /translation="MLPTNKWFQAKPPGVKRVLFHSINHTGLGHLNRSIAVAQLLKAD
                     MPDVQVLFMIEGGEDFIEPSGFPWMLIPGHNQSSEAENIEYITRTALEVYQPDLMIYE
                     TVLLESMRTPVREAGVKEALMGNMGGLLRNQVRDFIAGKHPVDLLLVLQQPEEVTPDD
                     RALVGQYTGRATYAGPLVRLKDQLSRESLRKKLGLADQHRVILLTFGGGGYDMVQQLL
                     TNLLSTRSQILAACPEAKLVVISGPYFSGELPDEDEFICRSSRFEPFLTDYIDIASAV
                     VCMAGYNTVNEIAVSGIPAVCVPVTEADDQVGRGSMGEYAERFPNMALGSMDTDTLAL
                     QVIEALSRDRDLSVTRAFRQRADGASRVIVNEMKRLLGVEQ"
BASE COUNT          369 a          430 c          428 g          384 t
ORIGIN      
        1 cttgccgctc ataatggcaa tgatatgttt tatgtgtcgc tcttcagttg tattcatcaa
       61 tgtttccatt gcacagagaa tcctttcatt gctcgacgcc taacagcctt ttcatctcgt
      121 tgacgatgac ccgcgaggct ccgtcggccc tctgccggaa agcccgtgtc accgagaggt
      181 ccctatctct tgacagggct tcgatcactt gtaaagccag agtgtcggta tccatgctgc
      241 ccagcgccat attggggaac ctctcggcat actcgcccat gctgcctcgt ccaacctggt
      301 catcggcctc ggtgacaggg acgcaaacgg cggggatacc cgagacggct atttcgttta
      361 ccgtgttgta gccggccata cagacgactg ctgaggcgat atcgatatag tcggtaagaa
      421 atggctcaaa acgactggaa cggcaaataa actcatcttc gtccggcaac tcgcctgaga
      481 aatatggccc gctaatcacc acgagtttcg cttcaggaca tgctgcgaga atctgcgacc
      541 tggtggaaag gaggttggtc aggagttgtt gcaccatgtc gtatccccct ccaccaaagg
      601 tcagcaaaat cacacgatgt tgatcagcca ggccgagctt tttgcgcaag ctctctcgcg
      661 aaagctggtc ttttaaacgc acaagcgggc cggcgtaggt cgctctgccc gtatactggc
      721 cgacaagcgc ccggtcgtcg ggggttactt cttccggttg ttgtaggacc aggagtagat
      781 caactgggtg cttgcccgca atgaaatcgc gcacctggtt gcgcaataga ccgcccatat
      841 tgcccattag cgcctcttta acgcccgctt ccctgacagg ggtgcgcatc gattcgagca
      901 ggacggtttc gtagatcatg agatcgggtt gatatacttc taacgcggtg cgggtaatgt
      961 attcgatatt ctctgcttct gacgactgat tatggccagg aattaacatc cacggaaacc
     1021 cgctcggctc gataaaatcc tcgcccccct cgatcatgaa gagtacctgc acatcaggca
     1081 tgtctgcctt caataactgg gcaacggcga ttgagcgatt caaatggccc aggccagtgt
     1141 ggttgatgga atgaaacaaa acacgcttta cccccggagg ctttgcttga aaccacttgt
     1201 tcgttggcaa catactcaca cctttctatc cgtgtcgcgg gggaggaatt ctatgcaatc
     1261 tgccttcccc tacctgatag acgttaagcg gcagggttcg taacacttcc tcgcgtggat
     1321 atgacccggt accaggggta gcccatatct gctgctgtgg gatgtttggc tagcgccgtg
     1381 agggaatgcc agatcgaacg ttataacaaa tatgcatcga tcaatgcaac gagcatagaa
     1441 tatgcagcca gcgggggagc attagcaggt ctacttgaga agggaaggac ctatgcgtct
     1501 ctgcgtagat ggagacgagt acagatgcca gaaattttgg atgatccagc cgggtcgggg
     1561 aagatgcgag ccgtcgtagt tgaagaccct gttcttccag tggcatcacc a
//
LOCUS       DASZAK010000792         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_588714, whole genome shotgun sequence.
ACCESSION   DASZAK010000792 DASZAK010000000
VERSION     DASZAK010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588714"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..1067
                     /locus_tag="VGT44_14025"
     CDS             183..1067
                     /locus_tag="VGT44_14025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581968.1"
                     /translation="MKTRSVAQGNASLVSSIFLVARRRENEDTGDYVSQVRPQLISII
                     NERLDTLIGAGVSGADLIIAIIGAGLRAYTQFARVEMPNGDELDAKTFLGEVEREVAQ
                     HVLQRLLAAAADGTELDAGSRVSAVDTVTRFYVTGRFFYGESAAPFDDMNLLARGMGV
                     ELDGPRGLAQGKKGLAKKEKDTVQLRDYRGRGDDENLGRPSESGNAAPLIDVLHRLLW
                     LQEEQPYGVQDFLLKTHPNIEQLRLVAQALAGHALAPAGDNGSTKQERTEEQKAVDRL
                     QAGWRGLFAEMSGSTLWG"
     gene            1117..>2700
                     /locus_tag="VGT44_14030"
     CDS             1117..>2700
                     /locus_tag="VGT44_14030"
                     /inference="COORDINATES: protein motif:HMM:NF016358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HEV2581969.1"
                     /translation="MVTQQQGATLPTWNRVVTPHIDIVQGQLSMDTYAVNLGKVVQQD
                     ESVRLVYRDARTFFEATYLTTELRRILTDVMEVLSGKSGNRVLQLRTPFGGGKTHTLL
                     SLYHLAKSRQMLNDLPELASLPDAGTVHVAVISGIETGAADTHSVQRRTIWGELAWQL
                     GKAEGYALVAEQDRLGIAPGAEVITRLIGSAPTLLLLDEVLTYVENAMGIIVGESTLG
                     RQTIIFLQRLTEAVAAAPKAAMVYSLQASEQEAGGNLEILGILSKLVQRLNAIREPVS
                     GDEVLRVVQRRLFSHLGDESQHQQIANAYAESYRGFLLAGSTSAQQAQQQSEQLRSRI
                     LLSYPFHPALLDLMRERWSALPSYQRTRGALQFLATVVHALWSGNIQSQPLLGPGDVP
                     LTDGQVRTTFLAQVGEQTQYDAVLQADMLGPHAGARSVDELMVQESPLLQAYLPGTRI
                     ASAALLYSFGGSSQIERGVFENELLSACLAPGLDRNILQTALRDLNERLLYLHRRELR
                     YRFETQPNLNKMIIDENQRR"
BASE COUNT          579 a          750 c          807 g          564 t
ORIGIN      
        1 tgtcatcaac tatggtaaaa atagtggtga gaacggtagc tttatcaagc atcggtagtg
       61 cgctccttgt ttgtctgtga tctttacaaa ccgaagcgta ctacacttct ccttctctgt
      121 cagccactag cacaaagcgt actggttttg tgattgttga agcttggccg ctagatacag
      181 aaatgaagac tcgatcggta gcacaaggta atgcttctct tgtctccagt atcttccttg
      241 tagcccgtcg tcgcgagaac gaagacaccg gtgactatgt gagccaggta cggccacagc
      301 ttatcagcat catcaatgag cgattggata cgcttattgg agcgggagta tccggagcag
      361 accttattat tgcaatcata ggcgcagggc ttcgagccta tacacagttt gctcgtgtgg
      421 agatgcccaa tggcgatgaa ctggacgcga aaaccttttt aggcgaggta gagcgcgaag
      481 tggcgcagca tgtcttgcag cgtttgctcg cagcggcggc ggatggcact gagttagacg
      541 ctggatcgcg cgtatccgct gtcgataccg tgacgcgctt ctatgtgacg ggcaggttct
      601 tttatggcga aagcgcggct ccttttgatg atatgaacct gctggcgcgc ggtatgggtg
      661 tggaactgga tgggccgcgc gggctggcac agggcaagaa ggggctggca aagaaagaga
      721 aggataccgt ccaactgcgc gactatcgcg gtcgcggcga tgacgagaac ctgggaagac
      781 ccagcgaaag cggcaacgct gctcctttga tcgatgtgct gcaccgtttg ctctggttgc
      841 aggaagaaca gccttatgga gtgcaggatt tcttgctgaa aacgcatccc aacatcgagc
      901 agttgcgcct ggtagcgcag gcgctagccg gtcatgcgct ggccccggcg ggagacaatg
      961 gcagcacgaa gcaagagcgc accgaagagc aaaaggcggt agatcgctta caggccgggt
     1021 ggcgcggcct tttcgcggaa atgtccggca gcacactgtg gggataacct ttttttgaag
     1081 cataggcgat gaatgggtat gtaaagggag agcattatgg taacacagca acagggcgcg
     1141 acgctgccta cctggaatcg cgtcgtcacg ccgcatatcg atatcgtgca gggtcagctt
     1201 tctatggaca cctacgctgt caatctgggc aaggtcgtcc agcaagacga gagcgtgcgc
     1261 ctggtctatc gcgatgcgcg cactttcttc gaggctacct atttgaccac cgagctccgc
     1321 cgcatcctca ccgatgtgat ggaggtgctg tcaggcaaga gcgggaatcg cgtgctgcaa
     1381 ctgcgcaccc cctttggcgg cggtaaaacc catacgctgc tctcgctcta tcacctggcg
     1441 aagtcacgcc agatgctgaa tgatttgcct gaactggcga gcttgcccga tgccggcacc
     1501 gtgcatgtgg cggtcatttc cggcatcgaa acgggcgcag ccgatacgca tagcgtgcag
     1561 aggcgcacca tctggggtga actggcgtgg caacttggca aggctgaagg ctacgcgctg
     1621 gtagcggaac aagaccgcct gggcatcgcg cccggcgcgg aagtcatcac ccgtttgatc
     1681 ggctctgctc ccacactctt attgctggat gaagtgctca cctatgtcga gaacgcgatg
     1741 ggcatcatcg tgggcgaatc gacgctggga agacaaacca tcatcttttt gcagcgcctc
     1801 acggaagccg tggcagccgc tcctaaagcg gctatggtct attcattgca ggccagcgag
     1861 caagaggctg gcggcaacct ggaaatactg ggtatcctga gcaagctcgt gcagcgactc
     1921 aacgccatcc gcgaacctgt cagcggcgat gaagtgctgc gtgtggtgca gcgccgcctt
     1981 ttctctcacc tgggtgacga gtcacagcat cagcagatcg ccaatgccta tgctgagagc
     2041 taccggggat ttttgctggc cggtagcact tccgcgcaac aggcgcagca gcagtccgaa
     2101 caactgcgct cgcgtattct gctcagctac ccctttcatc ccgcgctgct cgacctgatg
     2161 cgcgagcgtt ggagcgcgct gcccagctat caacgcacgc gcggcgcgct gcaattttta
     2221 gccacggtgg tacacgcgct ctggtcgggt aacatccagt cccagccatt actggggccg
     2281 ggcgatgtgc cgcttactga tggacaggta cgtaccacat tcctggctca ggtcggcgaa
     2341 cagacgcaat acgacgcggt gctacaggcc gacatgctgg gaccgcacgc cggtgcgcgc
     2401 agcgtcgatg aactgatggt gcaggaaagc ccactcttac aggcatacct gcccggcacg
     2461 cgcattgcca gcgccgccct gctctacagc tttggcggca gcagccagat cgagcgaggt
     2521 gtctttgaga acgaattgct cagcgcctgc ctcgcccctg gccttgatcg caatattttg
     2581 caaacggctc tgcgtgacct gaatgagcgc ctgctgtacc tgcaccgccg cgagctgcgc
     2641 tatcgtttcg agacgcagcc caacctcaat aaaatgatta tcgatgaaaa tcagcgccgt
//
LOCUS       DASZAK010000793         2543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_481426, whole genome shotgun sequence.
ACCESSION   DASZAK010000793 DASZAK010000000
VERSION     DASZAK010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481426"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VGT44_14035"
     CDS             <1..732
                     /locus_tag="VGT44_14035"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEV2581970.1"
                     /translation="IGAGCVAKVIAAWVGNVSEGLAHCYRRAIEKSIPRPLVTEEHSN
                     FTIGLALLAASLGSPYIPTRSLLGSDLPRQNPTLRIEPSMGDGSPLLLVPAIRPDVAI
                     LHVQRSDEEGNAHCWGSIGIGVEAMLAARDVILVAEEIVSRDIIISDPNRVIGPSFKV
                     RAVVHEPWGAHPSTAQGYSNRDHAYYHDYHMRTRTLEGFEEWLAEWVMALPDRAAYAR
                     KLGEERLRSLGVKEHRYAAPVDYGY"
     gene            743..1522
                     /locus_tag="VGT44_14040"
     CDS             743..1522
                     /locus_tag="VGT44_14040"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEV2581971.1"
                     /translation="MDYTARELMVVCAARQIRDGENVFVGMRLPLIAFALAKRTHAPN
                     CVGLFEGGIVRDMPSPELLYTMGDAPNIAGSLWATSTLNVMRPMATGDVHLGFIGGAE
                     VDRYGNLNTSLIGDWRHPKTRLPGSGGGADIASFSQRLAIIMQHDRHRLREKIDYITS
                     PGYGYPGEHSPAGIAWRKRVGLPRGGPAALITTLGVFTFDKETGEALLSSYHPGTMIE
                     EVQEQTGWELRLAEDCRETVPPSEEELRVIRECDPQGVWTR"
     gene            1550..2458
                     /locus_tag="VGT44_14045"
     CDS             1550..2458
                     /locus_tag="VGT44_14045"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV2581972.1"
                     /translation="MEINVITGAFSYTGKYITRHLLAMGKQVRTLTGHPDNFNPFGEQ
                     VQVFPFHFDDPAELIKSLEGATTLYNTYWIRFSYSDNTFERATARSKVLIDAAVEAGV
                     QRIVHVSITNPAIESRLPYFRGKAELEAHIMQSKLSYAIIRPTVIFGAEDILINNIAW
                     CLRHFPVYPIFGSGAYRIQPIYVEDMADLVVEAGQRRDNTCLDAIGPDIFTFEELVKL
                     VKTTLRSRAILLHVPPSFAYYAAKLIEPFVGDVLITRDEISGLMADLLVSQHQPTGHT
                     HLADWLMQNSAIVGTKYASELARHYR"
BASE COUNT          539 a          776 c          726 g          502 t
ORIGIN      
        1 atcggcgcgg gctgcgtcgc aaaggtcatc gcggcctggg tgggcaatgt cagcgaggga
       61 ctggcgcact gctaccggcg cgccatcgag aaaagtatcc cgcgcccgct agtgaccgag
      121 gaacatagca atttcaccat cggtcttgcg ctgctggcag ccagcctggg ttcgccctat
      181 atccccacgc gctcgttgct gggcagcgac ctgccgcgcc agaacccgac gctgcgcatc
      241 gagccatcga tgggcgatgg ctcgccgctg ctgctcgtcc cggccattcg ccccgacgtg
      301 gcgatcctgc acgtgcagcg tagcgacgag gagggcaacg cacactgctg gggcagcatt
      361 ggcatcgggg tagaggctat gctggccgcg cgagatgtca ttctcgtcgc ggaagaaatc
      421 gtctcgcgcg atatcattat cagcgacccc aaccgcgtca tcgggccatc cttcaaggtg
      481 cgcgccgtgg tgcatgaacc ctggggcgcg catccctcca ccgcgcaggg ctatagcaac
      541 cgcgaccacg cctactatca cgactaccac atgcgcacac gcactttgga aggtttcgag
      601 gagtggctgg ctgaatgggt catggcgctg cccgaccgcg ccgcttatgc gcgtaagctg
      661 ggcgaggaac gcctgcgcag cctgggcgtc aaagagcatc gctacgccgc gcccgtcgat
      721 tatggctatt aaggagggcc agatggacta taccgcgaga gaattgatgg tggtctgcgc
      781 ggcgcgccag attcgcgacg gtgagaacgt cttcgtgggc atgcgcctgc ccctcatcgc
      841 ctttgctctg gccaaacgca cacacgcccc caactgtgtc ggcttatttg aaggcggtat
      901 tgtgcgcgac atgccctcgc ccgaactgct ctacaccatg ggtgacgctc cgaacatcgc
      961 cggttcgctg tgggccacca gcacgctgaa cgttatgcgc ccgatggcaa caggcgacgt
     1021 gcatctcggc ttcatcggcg gcgcggaggt tgatcgctac ggcaacctca acacctcgct
     1081 gataggcgac tggcggcatc ctaaaacacg cctgcccggc agcggcggcg gcgctgacat
     1141 cgcctcgttc tcgcagcgcc tcgccatcat catgcagcac gaccgccacc gcctgcgtga
     1201 aaagatcgat tacatcacca gccccggcta cggctacccc ggcgaacaca gcccagccgg
     1261 aatagcatgg cgtaagcgcg tcggcctgcc cagaggcggc cctgctgctc tgattactac
     1321 cctgggcgtc ttcaccttcg ataaagaaac tggcgaggcg ctattatcgt cctatcatcc
     1381 tggtacgatg atagaagaag tacaggaaca aactggctgg gaattaaggc tcgcggagga
     1441 ttgtcgggag acggtgccgc cgagcgaaga ggaattaagg gtgatacggg aatgcgatcc
     1501 gcagggcgtg tggacgaggt aaaggccacc atattagaga ggacattcta tggaaattaa
     1561 cgtcatcacg ggcgcattca gctataccgg caaatatatc actcgccacc ttctggcgat
     1621 gggcaagcaa gtacgcacct tgacgggaca tcctgacaac ttcaatccat ttggcgagca
     1681 ggtgcaggta tttccttttc atttcgatga tcctgccgaa ctcataaaaa gcctggaggg
     1741 cgcaaccacg ctctacaata cctactggat tcgcttttcg tatagcgata acacctttga
     1801 gcgcgcaacc gcgcgctcaa aggtgttgat cgatgcggct gtggaggccg gagtgcagcg
     1861 tattgtgcat gtcagcatca ccaacccggc catcgagtcg cgactgccct attttcgagg
     1921 taaagcggag ctagaagcac acatcatgca gtcgaagctc tcgtatgcca ttattcgtcc
     1981 caccgtcatc tttggcgcgg aagatatttt gatcaacaat atcgcctggt gcctgcgcca
     2041 ctttcccgtc tatccgattt tcggctccgg cgcctatcgc atacagccga tctacgttga
     2101 agacatggct gacctcgtgg ttgaggcagg gcagcgtcgc gacaacacct gcctggacgc
     2161 catcggcccg gacatcttca ccttcgagga acttgtaaaa ctcgtcaaaa cgaccctgcg
     2221 cagccgtgcg atactgctgc atgtaccgcc atcgttcgct tactatgccg ccaaactgat
     2281 cgaaccattc gtaggtgacg tgttgattac tcgcgatgag atcagcgggc tgatggccga
     2341 cttgctggtt tcccagcatc agccaaccgg ccacacccac ctggctgact ggctcatgca
     2401 gaacagcgca attgtaggaa caaaatacgc ctcggaactc gccagacatt accgttaagt
     2461 cgtagggatg cgattttgat gacaatcaaa gccggaaatc cctcgtaagc gccggtgtac
     2521 cgtgcgcatc gccgatttat cgg
//
LOCUS       DASZAK010000794         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_551921, whole genome shotgun sequence.
ACCESSION   DASZAK010000794 DASZAK010000000
VERSION     DASZAK010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551921"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /gene="atpA"
                     /locus_tag="VGT44_14050"
     CDS             <1..1428
                     /gene="atpA"
                     /locus_tag="VGT44_14050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV2581973.1"
                     /translation="QDGIARVYGLQDVKYLELVEFPRTGLVGMAFNLEEETVSCPILG
                     EYTHLKEDDEVRTTGRIIEVPVGDALIGRVVNPLGEPLDDKGPIVTSRRRPVERIAPG
                     VIARQPVDTPVQTGLKAIDSMIPIGRGQRELIIGDRQTGKTAVAIDTIINQKGKNLIC
                     IYVAIGQKNSSVARTQAILEQHGAMDHTIIVAASAAEPAPLKYLAPYAGCSMGEEIME
                     SGRDALIIYDDLTKHAEAYRNMSLIIRRPPGREAYPGDVFYLHSRLLERAARLSKDFG
                     GGSLTALPIIETKANDISAYIPTNVISITDGQIFLETDLFNAGVRPAMNVGLSVSRVG
                     SSAQSRAMKQVAGSLRLDLAQFRELAAFAQFSSDLDKATKSRIDRGQRLTEILKQPQY
                     QPVPVEKQVMIIYAATNGYLDDVPVDKVTAWEAAFYRYMDANHPEVGEAIVEKSVKGK
                     DKMSDDLLKQLRAAIEEFKQTAAPN"
     gene            1440..2330
                     /gene="atpG"
                     /locus_tag="VGT44_14055"
     CDS             1440..2330
                     /gene="atpG"
                     /locus_tag="VGT44_14055"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEV2581974.1"
                     /translation="MPSTREIRRRIRSVKNTAQITKAMQMVASSKMRRAQERVQQSRP
                     YAEQLRTLVSHLASTSDADAGEEAALLKQRPVHNIGIVLITPDRGLAGALPSNINRKA
                     AATALEQQQKLAEQGERPDIGYVAVGRKGRDFVVRNRLHLLAEFTNYGDKPSLRDAAA
                     IAQVARDAFLKGEVDVVYLIYAKYINTVTQQPTAIQLLPVQPPAQYSSERVEYIYEPD
                     AQSIFQALLPRYVDVQVYQAMLEAVASEFSAKYVAMKNATDNANDLVQDLTLTYNKAR
                     QTSITTEILEVVSGANALES"
BASE COUNT          579 a          675 c          660 g          463 t
ORIGIN      
        1 caggatggca tcgctcgcgt ctacggcttg caggatgtga aatatctcga attggtagaa
       61 ttccctcgca ccgggctggt gggcatggcc tttaatcttg aggaggagac ggttagttgc
      121 cccatcctgg gcgagtatac acaccttaaa gaagatgacg aggtgcgtac caccgggcgc
      181 atcatcgagg tacccgttgg cgatgccctg atcggtcgcg tcgtgaaccc gctgggtgag
      241 ccactcgacg acaaaggtcc gattgtgacc agtaggcgcc gcccggtcga gcgtatcgcg
      301 ccaggcgtca ttgcacgcca accggtcgat acaccggtgc agaccggcct caaggctatc
      361 gatagcatga tccccatcgg gcgcggtcag cgtgaactga tcattggcga ccgccaaacg
      421 ggtaagaccg ctgttgctat cgacaccatc atcaatcaga agggcaaaaa tctcatctgc
      481 atctacgtgg caattgggca gaagaactcc tcggtcgcac gcactcaggc gatcctggaa
      541 caacatgggg caatggatca caccatcatc gtcgccgcaa gtgctgctga accggccccc
      601 ttaaagtatc tggcccccta cgccggctgc tctatgggcg aggagatcat ggaatctggg
      661 cgcgatgcgt tgatcatcta tgatgacctg accaaacacg ctgaggccta tcgcaacatg
      721 tcactgatta ttcgtcgtcc acctggacga gaagcgtatc ctggtgacgt cttctacctg
      781 cactcgcgct tattggagcg tgctgctcgt ttgagcaagg atttcggtgg cgggtcgctg
      841 accgcgttac ctatcatcga aacgaaagcc aacgacatct cagcctatat tccaaccaac
      901 gtcatctcga tcactgatgg acagatcttc cttgagactg acctgttcaa cgcaggcgtg
      961 agaccggcca tgaacgtcgg cctctcggtc tcccgtgtag gcagtagtgc ccagtcacgc
     1021 gctatgaaac aggtggcagg atcgctgcgg cttgacctgg cacagttccg cgagctggct
     1081 gcctttgcgc agttctcttc ggacctggat aaggcgacca aaagccgcat tgaccgcgga
     1141 caacgcttga ccgaaatact caagcagcca cagtaccagc cggtaccggt cgaaaaacag
     1201 gtcatgatca tttatgcggc cactaacggg tacctggacg atgttccggt ggacaaggta
     1261 acagcctggg aggcagcgtt ctaccgctat atggacgcca accacccaga ggtcggagaa
     1321 gccattgtcg agaaatccgt caaggggaaa gacaagatgt cggacgattt gctgaaacag
     1381 ttgagagccg ccatcgaaga gttcaagcag acggcagcgc cgaactagga gacagcacta
     1441 tgccatcaac ccgagagatc aggcggcgta taaggtcggt caaaaataca gctcagatca
     1501 ccaaagcaat gcagatggtg gcgagtagta agatgcgacg agcacaggag cgcgtccaac
     1561 aatcgcgccc ctatgccgag cagcttcgaa cgctcgtctc acatctggct agcacctcgg
     1621 atgccgatgc gggtgaggaa gcagcactgt tgaagcagcg cccggtacat aacattggca
     1681 ttgtcttgat cacaccggac cgcggcctgg ctggggcctt gccgagcaac atcaaccgca
     1741 aggcagccgc cactgcactg gagcaacaac agaaactggc ggagcagggc gagcgcccgg
     1801 atattggcta cgtcgccgtc ggtcgtaaag gacgagattt cgttgtacgc aatcggctgc
     1861 atctgctggc agagttcacc aactatggcg ataaaccatc gctacgggat gccgcggcca
     1921 tcgcccaggt ggccagagac gccttcctaa aaggggaggt cgatgtggtc tacctgatct
     1981 atgctaaata catcaacacc gtgacacagc agccgaccgc tatacagttg ttaccggtgc
     2041 agccaccggc acaatacagt agcgaacgtg ttgaatatat ttacgagccg gacgcgcaga
     2101 gtatcttcca ggccctgctc ccgcgctacg tggatgtaca ggtctaccag gcgatgcttg
     2161 aggccgtggc aagcgagttt tcagcaaagt acgtggccat gaaaaacgcc accgataacg
     2221 cgaacgatct ggttcaggac ctcactctca cctataacaa agctcgccag acaagcatca
     2281 ccaccgagat ccttgaagtc gtctccggtg caaatgcgct agaatcgtaa ctattcggga
     2341 atcctcacca ccttcggtga ttcacaagag gtttttt
//
LOCUS       DASZAK010000795         3353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_625256, whole genome shotgun sequence.
ACCESSION   DASZAK010000795 DASZAK010000000
VERSION     DASZAK010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3353
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625256"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VGT44_14060"
     CDS             <1..214
                     /locus_tag="VGT44_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581975.1"
                     /translation="SIAGLLVILFFPDVLGEFVLSAVVPLAGTVLGIPIDAGFDWIAF
                     ALAIVSLLRFFPAELVAEHYRYVFRK"
     gene            complement(497..2434)
                     /gene="dxs"
                     /locus_tag="VGT44_14065"
     CDS             complement(497..2434)
                     /gene="dxs"
                     /locus_tag="VGT44_14065"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEV2581976.1"
                     /translation="MTRILDSITNPSQLRALSQAQLRQLAEEIRQEIIEKVSVKGGHL
                     APNLGVVELTMALHMAFDTPHDLLVWDIGHQAYVHKLLTGRRERFHTIRQFGGLSGYL
                     RREESEYDVFGASHASTSISAALGLAVARDLKGEDFKVVAVIGDGALTGGMALEAINN
                     AGSLKKNLIIVLNDNEKSISDNVGAIHLSLSKARHMETLPSYRRLREMAKGSINRLPV
                     VGEKAREAAGRAEMSFKNFVMHSKTGQIFEELGIKYFGPFDGHDLPLMLDVFENIKRI
                     EGPVIVQVITKKGKGWEFAEQDATKWHGPGAFDYTTGTIKKTAGDPPTYTDVFADTLV
                     NIAEKDERVVGITAAMAEGTGLKKMRQRFPERYFDVGIAEQHAVTFAGGLAVGGLTPV
                     VAIYSTFMQRAIDQAIHDICMQNLHVVFAMDRAGVVGEDGPTQHGVFDIAFMRMIPNM
                     KVMAPKDENELRHMLYTAINMDGPVSLRYPRGKALGVPLDADLRKLEIGKAELVSPDT
                     IDAVEQQDCAILAYGTTVALAEEAARELAAEGIRAAVINARWAKPLDEALILRLAKRM
                     HRLITVEDHMVAGGFGSAVLELLEAHNLLREVDVRVIGYPDRLIEHGAPSILKELYGL
                     TASHIKDVVRALVATRAEAHP"
     gene            2773..3330
                     /locus_tag="VGT44_14070"
     CDS             2773..3330
                     /locus_tag="VGT44_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581977.1"
                     /translation="MTRKDEKDTERPHYYSQFWLDVAAGRREIGSSKTDESADSDDLP
                     ELTTTRRGSRPTPIVLSDGHRNSRAHDFEEDDDFSEPEVEEEEDDFSEPEVEEDELAG
                     DLDGENTPDILFGDTSVPAEVEPVAEEQPTDEEAVEEEEYFDEEEEEDEDGWAARGRK
                     KPKPGRQAKTPKAPLKKPKRGGRAY"
BASE COUNT          706 a          955 c          999 g          693 t
ORIGIN      
        1 ctcgattgcc ggcttactgg tcatcctgtt cttccccgat gtgctcggtg agttcgtgct
       61 gagtgctgtt gtcccgctgg ccggaacggt tctcggcatt cccatcgatg ccggttttga
      121 ctggatcgcc tttgccctgg ctatcgtttc gctgctgcgc ttcttccccg ctgaattagt
      181 agcagagcat taccgctacg tctttcgcaa ataacgctcc atgatggcgt cccctgttga
      241 tctgcgaatc gctggccgtg agatcgtaca ttggccacca gggacgcggg cgacgcaggc
      301 gtccctatag gcgttaactt aaagaccatg cctgtcatcc tgagcgcagc gaaggatctc
      361 ggtccccggc gcgtcagatc cttcgctgcg ctcagagctt gccctgaacg aagtgaaggg
      421 atgacatccg acgacccgtc gcgcaaggcg atcgcccagg aaggtcagct taagctaacg
      481 cttatgaccc ccgcccctat ggatgcgcct ccgctcttgt ggctacgagg gcgcgcacca
      541 catctttgat gtgggacgcg gtgaggccgt agagttcttt gagaatagag ggcgcgccgt
      601 gttcgatcag gcgatcggga tagccgatga cgcgaacatc gacttcgcgc agcaggttgt
      661 gcgcctcgag cagttcgagg acggcgctgc cgaagccgcc cgcgaccatg tggtcctcaa
      721 cggtgatgag gcgatgcatc ctcttcgcca gtcgcagaat gagcgcttca tcaagcggct
      781 tggcccagcg cgcgttgatg accgccgcgc gaataccctc ggcggccagt tcccgcgcgg
      841 cttcctcggc gagcgcaacg gttgtgccat aggccagaat tgcgcagtcc tgctgctcga
      901 ccgcatcaat ggtgtcaggg ctgaccagct cggctttgcc aatttccagc ttgcgcagat
      961 cggcatccag ggggacgccg agggctttgc cgcgcgggta gcgcaggctg acggggccat
     1021 ccatgttgat ggcggtataa agcatatgac gcaattcgtt ctcgtccttc ggcgccatga
     1081 ccttcatgtt ggggatcatg cgcatgaagg cgatgtcgaa gacgccgtgc tgtgtcggac
     1141 cgtcttcgcc gacgacgcca gcgcggtcca tggcgaacac cacgtgcaga ttttgcatac
     1201 agatgtcatg gatcgcctgg tcgatggcgc gctgcatgaa ggtagaatag atggcaacaa
     1261 cgggcgtcag gccgccgaca gccaggccac ctgcaaaggt gacggcgtgc tgctcggcaa
     1321 tgcccacgtc gaagtagcgt tcggggaagc gctggcgcat cttcttgagg ccggttccct
     1381 ccgccatcgc cgctgtaatg cctacgacgc gctcatcctt ttcggcgata ttgaccagtg
     1441 tatcggcgaa gacatcggta taggtcggcg ggtcgcccgc ggtcttcttg atggtgccgg
     1501 tcgtatagtc gaacgctccg gggccgtgcc acttggtggc gtcctgttcc gcgaactccc
     1561 agcccttgcc cttttttgta atgacctgta cgataaccgg cccctcgatg cgcttgatat
     1621 tctcgaagac atccagcatc aggggcaggt catggccatc gaaagggcca aagtacttga
     1681 tgcccagttc ttcgaagatc tggccggtct tactgtgcat cacgaaattc ttaaagctca
     1741 tctcggcgcg gccagccgcc tcgcgcgcct tttcgccgac gaccggcagg cgattgatcg
     1801 accctttggc catttcgcgc aggcggcgat agctgggcag cgtctccata tggcgcgctt
     1861 tgctgagcga gagatggatc gcgccgacgt tatcagagat ggatttttca ttgtcattga
     1921 gaacgataat cagatttttc ttgagactgc ccgcgttatt gatcgcttcg agcgccatgc
     1981 cgccggtgag cgcgccatcg ccgataacgg ccacgacttt gaagtcttcc cctttcaggt
     2041 cgcgcgcaac ggccaggccg agcgcggcag aaatcgaggt gctggcatgg ctcgcgccga
     2101 atacatcata ctcgctctcc tcgcgccgca gatagccgga caggcccccg aactggcgaa
     2161 tggtatggaa gcgttcgcgc ctgccggtca ataatttatg tacatacgcc tggtgtccta
     2221 tatcccagac cagcaggtcg tgtggggtat cgaacgccat gtgcagggcc atcgtcaatt
     2281 caacgacgcc caggttgggc gccaggtgtc cgcctttcac cgaaactttt tcgatgatct
     2341 cctgccggat ttcttcggca agctgtcgta gttgcgcctg gctcaaagcg cgcagctgcg
     2401 atgggttggt aatactatcg agaatgcgtg tcaatgtttc ctccaatcgg atgacaagcg
     2461 cccgtacttc aagataagac gctgctgttt ctcggagagg gtctatagaa tgtcaacctt
     2521 tattataggc cgcttgagaa agaggctgca aacgctccaa acggaaaatt catgcacatg
     2581 gggtcttgac aggccatcac cccgcctgga gagcgcatcc agtacataaa aagtgactct
     2641 tgacagcttg cgcaattctt tttatgatga gcgcatagat gccacgaagt ggtatcgcag
     2701 tacggtaaaa cattctggta gggcgtcctt ttgacaaaga caacgaacag accctacaga
     2761 aaaaggtgga gaatgaccag gaaagacgag aaagacacag agcgaccgca ttactattcc
     2821 cagttctggc ttgacgtggc agccgggcgc agggagatcg gttcatcgaa gaccgatgag
     2881 agcgccgaca gcgacgattt gccagaactc accaccactc gcagagggag ccgccccacc
     2941 ccgatcgtgc tgtcggacgg gcatcgcaac tcccgcgcgc acgatttcga ggaggacgac
     3001 gacttcagtg aaccagaggt ggaagaggag gaggacgact tcagtgagcc agaggtggaa
     3061 gaggacgaac tggcaggcga cctggacggc gagaacaccc ccgatatcct ttttggagac
     3121 acaagcgttc cggccgaagt agagccagtt gctgaggagc agcctaccga tgaggaggcg
     3181 gtagaagaag aggaatactt cgacgaagaa gaagaagagg acgaagatgg ctgggctgct
     3241 cgcgggcgca agaagcccaa gcccggtcgg caagcaaaga ctccgaaagc gccgctcaaa
     3301 aagccaaaac gtggcgggcg cgcgtattag tcagtaatgg caaatcgtca cca
//
LOCUS       DASZAK010000796         2325 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_552030, whole genome shotgun sequence.
ACCESSION   DASZAK010000796 DASZAK010000000
VERSION     DASZAK010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552030"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VGT44_14075"
     CDS             <1..837
                     /locus_tag="VGT44_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEV2581978.1"
                     /translation="TYAGLKALKGRYKNFTLGVHTVISNFNIDEFDNIYNFVHGELKP
                     DQFISEIAEERVELDTVGMGITPPIQKYQPVIERLQQEIRDTQFNGVARITEAFRDRY
                     YDIVKRTLAEKRQIIPCMAGVASAQIAPNGDVWACCIRAESMGNLRDYNYDFRSVWTT
                     AQAAEQRRSIKARECYCPLANASYTNMLCHVPTLTSVGLEVAKETVAAKLPAGKSGKS
                     ARLRSLPVLNAADEARIMNGDTNAKPALMRELPVLNASVPMPAHNGNGHHSDNSLIME
                     IE"
     gene            complement(843..1103)
                     /locus_tag="VGT44_14080"
     CDS             complement(843..1103)
                     /locus_tag="VGT44_14080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581979.1"
                     /translation="MNSQEKRDEGRVKLSKELPDQKRLLPGVQGDIAYELLMLQQRLD
                     AYEQLHLEEMAELRREMARLRRAFLQETTPQLRAIIPKRPEL"
     gene            1364..2041
                     /locus_tag="VGT44_14085"
     CDS             1364..2041
                     /locus_tag="VGT44_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2581980.1"
                     /translation="MSTMSESSQGGSPVIRVLVVDDHEIVRKGLCTMLGEQEDFEVVG
                     QAGNGGAAVSLAIELQPDIVLLDIFLGNTNGLDIAQQLQRACPDARIVIVTGLMDEGH
                     LFRAMRIGVHGFLQKALPIDELLAALRSVQRGERVVGEPRAMTQLLGEFERVTKEQER
                     GRSGLNALEIELVRLAAQGCSNKEIAARQFWSEVTVKRKMQDIYRKLQVTDRAQAVAE
                     AMRMGLI"
     gene            2061..>2325
                     /locus_tag="VGT44_14090"
     CDS             2061..>2325
                     /locus_tag="VGT44_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581981.1"
                     /translation="MTVCPQCGTENDDDVKNCRGCRINMYWAFQHYDELASLREANAL
                     PSRPQSATFLVQTSKKIDDGPTAPWLRSTIKKFGFKGAGKKVST"
BASE COUNT          517 a          666 c          691 g          451 t
ORIGIN      
        1 acctatgccg gtctcaaggc gctcaagggg cgctacaaga acttcacgct gggcgtgcat
       61 accgttatct ctaacttcaa catcgacgag ttcgacaaca tctacaattt cgtgcatggc
      121 gaactcaagc ccgaccagtt catcagcgaa atcgcagagg agcgcgtcga gctggatacc
      181 gtcggtatgg gcatcacgcc gcccatccag aagtaccagc cggtcatcga acgcttgcag
      241 caggagattc gcgacacgca gttcaacggc gtagcgcgca tcacggaagc cttccgcgac
      301 cgctactacg atatcgtgaa gcgcacactg gccgagaaac ggcagatcat cccctgcatg
      361 gccggtgtgg cctcggcgca gatcgcgccg aacggcgatg tgtgggcctg ctgcatccgc
      421 gccgagtcga tgggcaacct gcgcgactac aactacgatt tccgctcggt ctggacaacc
      481 gcccaggcgg cggagcagcg ccgcagcatc aaggccaggg agtgctattg cccgcttgcc
      541 aatgcctcct ataccaacat gctctgccac gtgccaacgc tgacgagcgt ggggctggaa
      601 gtggcgaagg agacggtggc ggcgaagctg cccgcaggca agagcggcaa atcggcccgg
      661 ctgcgcagcc tgccggtcct caacgccgcg gacgaggcgc gcataatgaa cggagatacc
      721 aacgcgaaac cggcgctgat gcgcgaactg cccgttctca acgcctcggt tccaatgccc
      781 gctcacaatg gcaacggcca tcacagcgac aacagcttga tcatggagat tgaataattc
      841 ggttataatt cggggcgttt ggggataatg gcgcgcagtt ggggagtggt ttcttgcagg
      901 aaggctcgcc gcagccgcgc catctcgcgc cgcagttcgg ccatctcttc aagatgcaac
      961 tgttcgtagg catccaggcg ttgttgcagc attaacagtt cataggcaat gtcgccctgc
     1021 acgccaggaa ggagccgctt ctgatccggc agttcctttg acagcttgac acgaccttca
     1081 tcgcgctttt cttgactgtt catggtgtcc cccgtgcgaa aaatactctt gcatttggat
     1141 gctattatct cgcatctttg tgcagcagga caatactaac cgtataggga aatcatatcg
     1201 ttcgtatcaa gcgcgcctat cccaccggct ttgctttagg aagaatatcc tatcatcaaa
     1261 tcgcgccggt gaaaaagtac gttgaataga gtgaggtgat gggacgatat atctgaatag
     1321 agcagactat ccttacgctg cgaccgactg gattggagtc ttgatgtcaa ccatgtccga
     1381 gtcttcccag ggaggctcgc ccgttattcg cgtcctggtg gtggatgacc acgagattgt
     1441 gcgcaaaggg ctgtgtacga tgttgggcga gcaggaagat ttcgaggtgg tcggccaggc
     1501 cggaaacggc ggcgctgccg tctcgctggc catcgaactc cagccggaca ttgtcttact
     1561 ggatatcttt ctgggcaata cgaacggcct ggacatcgcc cagcagttgc agcgcgcctg
     1621 ccctgacgcg cgcatcgtga ttgtgacggg tctaatggac gaagggcatc tttttcgggc
     1681 gatgcgcatt ggtgtgcatg gctttttgca aaaggcgttg cccatcgatg aactgctggc
     1741 ggcgctgcgt tccgtccagc ggggcgagcg cgtggtcggc gagccgcgcg ccatgacgca
     1801 gctcctgggc gaatttgagc gcgtcacgaa ggagcaggag cgcgggcgct ccggcctgaa
     1861 cgcgctcgaa atcgaactgg tgcgcctggc cgcccagggc tgtagcaaca aagaaatcgc
     1921 cgcgcgccag ttctggagcg aggtgacggt caagcgcaag atgcaagata tttaccgcaa
     1981 gctccaggtg acggaccgcg ctcaggccgt cgccgaggcg atgcgcatgg gtttaatata
     2041 gaaagaaggg agaaccaacc atgacagttt gcccacaatg tggaaccgaa aacgacgatg
     2101 atgtgaagaa ttgtagaggc tgccgcatca acatgtactg ggccttccag cactacgatg
     2161 aactggcctc gctgcgcgaa gccaacgcgc tgccgtcgcg accgcaatcc gcgacctttc
     2221 tcgtgcaaac gagcaaaaag atcgatgatg gcccgaccgc tccctggctg cgcagcacca
     2281 tcaagaagtt cggcttcaaa ggagccggca aaaaggtctc aacca
//
LOCUS       DASZAK010000797         2399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_695166, whole genome shotgun sequence.
ACCESSION   DASZAK010000797 DASZAK010000000
VERSION     DASZAK010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695166"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1408)
                     /locus_tag="VGT44_14095"
     CDS             complement(<1..1408)
                     /locus_tag="VGT44_14095"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing serine/threonine-protein
                     kinase"
                     /protein_id="HEV2581982.1"
                     /translation="MDQSANNDDMNALIGRSLGQYRIVDRIGAGGMATVFKAYQPTLD
                     RYVAVKVLPAYHARDPVFVKRFVQEARAVAKLQHAHIVPIHDYGEQENITYIVMEYVE
                     GGTLKDRLKLRQLTVLEAVDFIIQGAEGLGCAHSFGIVHRDVKPANMLLRKDSYLLLS
                     DFGIAKILEGTTNLTRAGTGIGTPQYMSPEQGTGQPVDRRSDIYSLGIVLFHALTGRV
                     PFTADSPITITVKHMNEPLPLEPLHSRGVPLPIEQVVVKMTAKSPADRYQTTDDLIMA
                     LTSALTASQLLLPRWRPGMQPSSPMQALPPTPTPVDVPQNQPPAQPPQQMPPNQPPQQ
                     QMPPAQNIPGNNNLGNLPPSASGNRAPMITVTCFRCGAANQSDRLFCTSCGYELSDSK
                     RLYDRAMGPNGRPILARLTVQNGQLAGRSYLFHQDVTTIGRTNGNDLVISGRTVSRRH
                     ARLWFDTNRWFIADMQSAN"
     gene            complement(1591..>2399)
                     /gene="sucB"
                     /locus_tag="VGT44_14100"
     CDS             complement(1591..>2399)
                     /gene="sucB"
                     /locus_tag="VGT44_14100"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEV2581983.1"
                     /translation="ANNASAMSAVPAMPATTPAPAAPPAGFGAGVTGQADRREERIRM
                     SRRRQTIAQRLVETQHAAAMLTTFNEIDMSAIMEVRNRRKESFKERHNVSLGFMSFFT
                     KAVVGALKAFPRLNAEIQGNEMILKQYYDIGIAVGAEEGLVVPVLRDADRRSFAEIER
                     EIADLAKRARANTLTLADLQGGTFTITNGGVFGSLLSTPILNGPQVGILGMHKIEQRP
                     VVVNSQIVARPMMYVAVSYDHRIVDGREAVQFLVRVKELLEDPETLLLEG"
BASE COUNT          486 a          680 c          702 g          531 t
ORIGIN      
        1 cgttggcgct ctgcatgtcg gcaataaacc agcgattggt gtcgaaccag agccgcgcat
       61 gtcgccgcga gacggtgcgc ccggagatca ccaggtcgtt gccgttggtt cgcccgatcg
      121 tcgtgacatc ctggtggaag agatacgagc ggccggcgag ctggccattc tgcaccgtta
      181 gtcgagccag gattggtcga ccatttggtc ccatcgcgcg gtcgtagaga cgcttgctgt
      241 ctgatagctc gtagccacag gacgtgcaga acaggcggtc gctctgattt gccgcgccac
      301 agcggaaaca ggtcacagtt atcattggcg ccctgttgcc gcttgccgat ggaggcaggt
      361 taccgagatt attgttccca ggtatatttt gagcaggcgg catttgttgc tgcggcggtt
      421 gattgggtgg catttgctgc ggtggctggg caggaggctg gttctggggt acatcaaccg
      481 gcgtcggtgt cggcggcagc gcctgcatcg gggacgacgg ctgcatacct ggtctccagc
      541 gtggcaaaag gagttgggac gcagtaaggg ccgaggtcaa cgccataatc aagtcatcgg
      601 ttgtctggta gcgatcagct ggagatttgg cggtcatctt gacgaccacc tgttcgattg
      661 gcagcggtac gcccctgctg tgcaaaggtt cgagcggcag aggctcgttc atatgcttga
      721 ctgtgatggt aatggggctg tcggcagtaa aggggacacg tccagtcaag gcatggaaga
      781 gaacgatccc cagcgaataa atatcgctgc ggcggtcaac gggctgcccg gttccttgct
      841 ctggagacat gtactgaggc gtaccaatgc ccgttccggc gcgtgtcagg ttggtcgtgc
      901 cctccagtat cttcgcaatg ccaaagtcgg agagcagcag gtaactatct ttacgcagca
      961 acatgttggc cggcttgaca tcgcgatgca cgatgccgaa gctatgcgcg cagccgagtc
     1021 cttctgcccc ctggataatg aaatcgaccg cttccagcac cgttaactgg cgcagtttga
     1081 ggcgatcttt tagcgtaccg ccctcaacgt attccattac gatataggta atattctcct
     1141 gctcgccata atcatgtatt ggcacaatgt gggcatgttg tagcttggcg accgcgcgcg
     1201 cttcctgcac aaagcgcttg acgaacacgg gatcacgagc gtgataggca ggcaacacct
     1261 tcacagccac gtagcgatcg agtgtcggct gataggcctt aaacaccgtc gccatgccac
     1321 ctgcgccgat ccggtcgaca atgcgatatt ggccgagcga tctcccaatc agggcattca
     1381 tatcatcatt gtttgccgat tgatccatag tcagacatat cctctatcgt accattcgcg
     1441 tatactacta tcatttttcg gacaagaaat caccctcacg ataaaacagg gcgatatcac
     1501 tgcattatta tacaggctga aacgcccatt gtcgagggga ttatgacgct ctaaccaggg
     1561 tagctggaaa ggtcgccctg gactcttctt tcatccctcc aaaagcagcg tctccgggtc
     1621 ctcgagtagt tctttgacgc gcaccaggaa ctgcacagct tctcgaccat ccacgatacg
     1681 atgatcgtag gagacggcga cgtacatcat tgggcgcgcg acgatctgac tattgacgac
     1741 aaccggacgc tgttcgattt tgtgcattcc cagaattccc acttgcggcc cattcaagat
     1801 gggcgtggag agcagcgagc caaacacgcc cccgttggtg atggtgaagg tgccgccctg
     1861 tagatccgcc agcgtcaggg tattggccct ggcgcgtttg gcaagatcgg ctatttcgcg
     1921 ctcgatctcg gcgaagctcc tgcgatcggc atcacgcagc accggcacga ccagcccctc
     1981 ttccgcccca acggcgatgc cgatatcgta atactgtttc aggatcatct cattcccctg
     2041 gatttcggcg ttcagacgcg gaaaggcttt gagcgcgcct accaccgcct tcgtgaagaa
     2101 ggacatgaac ccgagcgaga cattgtggcg ctccttgaac gactccttgc gccggttgcg
     2161 tacctccatg atcgcgctca tgtcgatctc gttgaaggtg gtcaacatgg cagcggcgtg
     2221 ctgcgtttcc accaggcgct gcgcaatcgt ctgacggcga cgcgacatgc gtatgcgctc
     2281 ttctcgcctg tccgcctggc ccgtcacgcc tgcgccgaaa ccggcgggcg gcgcggcagg
     2341 cgcgggcgtc gtggcaggca tagctggtac agctgacata gcagaagcgt tattcgcct
//
LOCUS       DASZAK010000798         5309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_481552, whole genome shotgun sequence.
ACCESSION   DASZAK010000798 DASZAK010000000
VERSION     DASZAK010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481552"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1382)
                     /locus_tag="VGT44_14105"
     CDS             complement(201..1382)
                     /locus_tag="VGT44_14105"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2581984.1"
                     /translation="MGDSFYAVALPWLVLTQGGNAQELGIILSAYGIPRIGSILLGGI
                     LSDRLRPRRVMLIADAARAVLVGILAALALVGHPAMWTLIAVAIPLGAFGGLFLPPSF
                     SILPDVLDDQDLQAGNALNTTSQQLAGLVGTGTGGIVVAAVQSGMALVIDALSFVVSA
                     VSLALIRGNAASRPTPSPTSPQAAKTGEDTQVEEGSEAEQPMTFWQLARTSPVVQTAF
                     IITLFANLTFGGLFEVALPSLAHGPLAAGATGYGAILAAFSAGALLGGIVTGMVKNIP
                     RRGVVALGVSLLQAIAIAFVPYAGLAGAITFMAVMGIFNSVTNVIFITIIQQIIPRHL
                     LGRVMGVIMFGSLGSYPLSVVIVGVVVARAGPSIIFPIGGAMLFLAVLYGLFQKQIWE
                     L"
     gene            complement(1449..3113)
                     /gene="metG"
                     /locus_tag="VGT44_14110"
     CDS             complement(1449..3113)
                     /gene="metG"
                     /locus_tag="VGT44_14110"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV2581985.1"
                     /translation="MNEHILVCVAWPYAKSSTHVGQIVGAYLPADTFARYHRLAGNKV
                     LMVSGSDEHGTPILVDSEREGVSPREFVARYHKQICEVWERLGMSWDLYTETGTENHY
                     RITQDFFLTLHDKGYIYKDSMLSPYCPTDRRFLPDRYVEGTCPHCGYTSARGDQCDNC
                     GRTLDPVDLIEPRCRLCGSKESKLEIRSTEHFFLDLPKLQDRLMAWLDDGKDFWRTNT
                     LGFAKNWLREGLRPRAITRDLDWGVPIPMEGYEDKRIYVWFDAVIGYFSASVEWAERQ
                     GTPEAWKTWWVPGQAQPPVKSYYFIGKDNIPFHAIIWPAMLMGYGNRNLPYDVPANEF
                     MTMSGAKASASKGNVIWTKDVLDRYSADTLRYYLSVTAPEGRDTDFTYEELIRRNNDE
                     LVATYGNAVHRTLTFLQSKFDGLVPQPQALSEADREILAEVDRGFTLVGHNIANCHFK
                     DGLIAAMSVARAANRYLDEQAPWKRIRQDRDAAGTTLYVMLQVLSGLHLMLWPYLPFS
                     SEKLHHFLGFTGDVRAAHWHPTEVPAGMKLPTPTPLFPKLEEVAAV"
     gene            complement(3464..4345)
                     /locus_tag="VGT44_14115"
     CDS             complement(3464..4345)
                     /locus_tag="VGT44_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2581986.1"
                     /translation="MTQPVSSEENSNRYVIDAEDATEMARLLDQDKQMTGSMGGIFSE
                     RSENDLANIHRVLDLACGPGGWVNEVSNAFPTMNVIGVDLSDKMIGYARAYAEVRKLA
                     NASFKVMDVTKPLDFADESFDLVNARCIFAFMSPQGWASLLAECKRILRPGGVIRLTE
                     GEWGVGTSPAYETYLGYFFKAMQLAGRSFSATGQHVGITPVLGHMLRDAGFEHIQKKA
                     HVHDFSYGEEAHPGFTFDLTLLFKLLQPFITRTGVATQAQLDTLYEKVQQEMRSEEFC
                     ALAYYLTVWGEKPNNGS"
     gene            complement(4492..>5309)
                     /locus_tag="VGT44_14120"
     CDS             complement(4492..>5309)
                     /locus_tag="VGT44_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEV2581987.1"
                     /translation="ILFDEDDIARRLPKAVAAAEKLRRINSDVTVQALVEDINADGIE
                     ALVQDVELVLDATDNFETRYLLNDACLKYGRPWIYSGVIASYGVTMNILPGDTACLRC
                     VYPEMPLPGTTATCDTAGILNGIVGAITGVVSTEALKIVLQSDKVSRDMFWMDVWENT
                     SERIELPRQADCPACGQHTYEFLESLSGSHTTSLCGRDAVQVRSSRKGDRVEFPTLAE
                     RLQAVGQVSYNAYLLRFLVDSYEFTIFPDARAIIKGTDDEMVARSLYARYIGM"
BASE COUNT         1077 a         1516 c         1608 g         1108 t
ORIGIN      
        1 ctgctctttg agcgcgcctc gcaccgcgct cgcaccgtcg cgctgtccgc gcccgccggt
       61 aaaacaaaga tcatccgcgc caaacttggc gacttttcgg gcgtcgtcgg cgcagcctgc
      121 ctgggcgcgg aagcggcggg gtatacgttc cctattgtgt aaagtgagaa gggcgcaccc
      181 ttgcggtcgc gcttctcact tcatagctcc catatctgct tctggaacag tccgtacagc
      241 accgccagga agagcatcgc gccgccgatg gggaagatga tactcggacc cgcgcgcgcg
      301 acgaccacgc ccacgataac caccgagagc ggataggagc caagcgagcc gaacatgatg
      361 acgcccatga cgcgccccag caggtgacgc gggatgattt gctggatgat ggtgatgaag
      421 atgacgttgg tgacgctgtt gaaaatgccc atgactgcca tgaaggtgat tgcgcctgcc
      481 agacctgcgt agggaacgaa cgctatggca atcgcttgca gcagcgacac acctaaggcg
      541 acgacgccgc gacgcgggat gttcttcacc atgcccgtca caatgccgcc aagtagagcg
      601 cctgcgctga aggctgccag gatcgcgcca tagccggttg cgcccgctgc cagcggcccg
      661 tgcgccaggg atggcagggc aacctcgaat aatccgccga aggtgagatt ggcgaagagg
      721 gtgatgatga aggctgtctg gacgaccggc gaggtccgcg cgagttgcca gaacgtcatg
      781 ggttgttcgg cttccgatcc ttcctcaact tgagtgtctt caccggtctt tgcagcctgt
      841 ggagaggtgg gagagggggt tggcctggat gctgcatttc cgcgaatgag cgccagcgag
      901 accgccgaca cgacgaacga gagcgcgtcg atgaccaggg ccatgccgga ttgcaccgct
      961 gccacaacga tccctccggt tcctgtccca accagccccg ccagttgttg ggaggtggtg
     1021 ttgagggcgt tgcctgcttg caaatcctgg tcgtccagca cgtccggcag gatggagaac
     1081 gagggaggca agaacagtcc cccgaacgcg ccaagcggga tggcgacagc gatcagggtc
     1141 cacatcgccg ggtgtcccac gagcgccagc gcggccagga tgcccaccag cacggcacgc
     1201 gccgcgtctg ctatcagcat cacgcgccgg ggacgcagcc ggtcgctcag aatgccgccc
     1261 aacaagatgc tgccgatgcg cggtatgcca tacgcgctga ggatgatgcc aagttcctga
     1321 gcgttgccgc cctgtgtcag caccagccag ggcagggcca ccgcgtaaaa ggagtcgcca
     1381 attgtggaag cgaattattg aatgtgacca ccgcgtcctt acgacggttg ccagatggcg
     1441 ttcccatatc ataccgccgc tacttcttcc agcttgggga agagcggcgt gggggtgggc
     1501 agcttcatgc ctgctgggac ctcggttggg tgccagtggg cggcgcggac atcgccggtg
     1561 aaacccagga agtggtgcag cttctcggac gagaagggca ggtagggcca gagcatgaga
     1621 tgcaggccgc tcagcacttg cagcatgaca tagagcgttg tgccagccgc gtcgcgatcc
     1681 tgtctgattc gcttccaggg cgcttgctcg tccaggtagc ggttggctgc gcgcgcgacg
     1741 gacatggcgg cgatgaggcc atccttgaag tggcagttgg cgatgttgtg gccgaccagc
     1801 gtaaagccgc gatccacttc ggccaggatt tcgcggtcgg cctcgctcag cgcctggggt
     1861 tgtggcacca gaccgtcgaa cttgctctgc aagaacgtca gggtgcgatg cacggcgttg
     1921 ccataggtcg cgaccagctc gtcgttattg cggcggatca gttcctcgta ggtgaagtcg
     1981 gtgtcgcgcc cttcgggcgc cgttactgag agatagtagc gcagcgtatc ggcactgtag
     2041 cggtccagca catctttcgt ccagatcacg ttgcctttac tggcggaggc tttcgcgccg
     2101 ctcatcgtca tgaattcgtt ggccggcacg tcatagggca ggttgcggtt gccgtagccc
     2161 atcagcatgg cgggccagat gatggcatgg aagggaatgt tgtctttgcc aatgaagtag
     2221 tacgacttca ccggtggttg tgcctggccg ggaacccacc aggtcttcca ggcttccggc
     2281 gttccctgcc gttcggccca ttcgaccgag gcggagaagt agccgatcac ggcgtcaaac
     2341 cagacgtaga tgcgcttatc ctcgtagccc tccatcggga tagggacgcc ccagtccagg
     2401 tcgcgggtga tggcgcgcgg gcgtagcccc tcgcgcagcc agtttttcgc gaagcccagc
     2461 gtgttcgtac gccagaaatc tttgccgtca tcaagccagg ccatcagcct atcttgcagt
     2521 ttcggcaaat ccaggaagaa atgctctgtc gagcgaatct ccagcttgct ctccttgctg
     2581 ccgcacaggc ggcagcgcgg ctcgatcaag tccaccgggt cgagcgtgcg tccgcagttg
     2641 tcgcactggt cgccgcgcgc cgaggtgtag ccgcagtggg ggcaggtgcc ttcgacatag
     2701 cggtcgggca ggaaacgccg gtcggtcggg caatagggag acagcatcga atctttgtaa
     2761 atgtagcctt tgtcgtggag cgtcaagaag aaatcctgcg tgatgcggta gtgattctcg
     2821 gtccccgtct cggtataaag atcccacgac atgccgagcc gctcccagac ctcgcagatt
     2881 tgcttgtgat agcgcgccac gaattcgcgc gggctgacac cttcgcgctc ggagtctacc
     2941 agaatagggg tgccatgttc atcgctgccg gataccatca acaccttgtt tcccgccaac
     3001 cggtgatagc gggcaaaggt atcggcaggc aaatacgccc cgacgatctg accaacatgg
     3061 gtggaacttt tcgcatatgg ccacgccaca cacaccagaa tatgttcatt catgataaga
     3121 gctatctccc attgagtata tcaagcaagg gtgtaagatt tatatgataa caaaacttgt
     3181 aggggccagt tttgtgtatt catagctgat tatgtacatt gacgaaatat tgcggtcgcc
     3241 ctggagctag ctttgcgatg tccgctttat ttattcaatg ttcattactg gccagtccag
     3301 ttagccggat aggcgaggat tcgcgccgat caatcggctt tgagcacggt aaaccggctc
     3361 ctacgagttt ttgtaatcag atagttctta cacctttcaa gcaacggcag cttgcagtaa
     3421 cttcgcgtat tataacatgc actgacaaga cggggcatca aggttatgaa ccgttgttgg
     3481 gtttttctcc ccataccgtg aggtagtacg ccagcgcgca aaactcttcc gaccgcattt
     3541 cttgctgtac tttttcatag agcgtgtcca gttgtgcctg cgtggcgacg cccgtcctgg
     3601 tgatgaaggg ctgcaggagc ttaaagagca gcgtgagatc gaaggtgaaa ccaggatggg
     3661 cttcttctcc gtaggagaaa tcgtggacgt gggctttttt ctgaatatgt tcgaagccgg
     3721 catcacggag catatgtccc agcacgggcg tgatcccgac gtgttgcccg gtcgcggaga
     3781 aactgcgccc tgccaactgc atcgctttga agaaatatcc caggtaggtc tcataagcgg
     3841 gtgatgtgcc aacgccccac tctccctcgg tgagccggat cacgccccct ggcctgagga
     3901 tgcgcttgca ttccgccagc aggcttgccc atccctgtgg agacatgaag gcaaagatac
     3961 aacgggcgtt gaccaggtca aaggactcat cggcgaaatc aagcggtttg gtgacatcca
     4021 tcaccttgaa cgaggcgttc gccaacttgc gcacctcggc ataggcgcgg gcgtatccga
     4081 tcatcttgtc gctgagatcg acgccaatga cattcatcgt ggggaaggca ttggacactt
     4141 cgttcaccca tccaccaggg ccacaggcga ggtcgagtac gcggtgaatg tttgcaagat
     4201 cattttcaga tcgttccgaa aagatgccac ccatactgcc ggtcatttgt ttatcctggt
     4261 cgagcaggcg agccatctct gttgcatctt cagcatcaat gacgtagcga tttgagttct
     4321 cttcagaaga tacgggttgt gtcatgagat ttgtccttct atgatgatct gcgaagagac
     4381 gcgagcggaa tctttgcacg cctgtaagtt catgcacgtg ttgctttaat cgtccatcga
     4441 tgccgctact taacacaaga atagcagaag aagcagtgga acgtcaatca tttacatccc
     4501 gatatatcgc gcgtataggg aacgcgccac catctcgtcg tcggtgcctt tgatgatggc
     4561 cctggcgtcg ggaaagatgg tgaattcgta ggaatcgacg aggaagcgca gcaggtaggc
     4621 gttgtacgag acctgcccga ccgcttgcag gcgctcggcc agcgtgggga attcgacgcg
     4681 atcacctttg cggctgctgc gcacctgcac ggcgtcgcgc ccgcacaggc tggtcgtatg
     4741 cgagccgctc aggctctcca gaaattcgta ggtgtgctgg ccgcaggcgg ggcaatcggc
     4801 ttgacggggc aattcgatgc gttcgctggt attctcccac acatccatcc agaacatatc
     4861 gcggctgacc ttgtcgcttt gcagcactat cttcagcgcc tcggttgaga caacgccggt
     4921 gattgcgcca acgataccgt tgaggatgcc ggcggtatcg caggtcgctg tcgtgccggg
     4981 caggggcatc tccgggtaga cgcagcgcaa gcaggcagta tcgccgggaa ggatattcat
     5041 cgtcacgcca taggacgcga tcacgccgct ataaatccag ggtcgcccgt atttgaggca
     5101 ggcgtcgttc agcaggtagc gcgtctcaaa gttatcggtg gcatccagca ccagctcgac
     5161 atcctgaacc agcgcctcga tgccgtccgc gttgatatcc tcgaccagcg cctgcaccgt
     5221 gacatcgctg ttgatgcggc gcagtttctc cgcggcggcc accgctttcg gcaacctgcg
     5281 cgcgatatcg tcctcgtcga acagaatct
//
LOCUS       DASZAK010000799         2127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_552107, whole genome shotgun sequence.
ACCESSION   DASZAK010000799 DASZAK010000000
VERSION     DASZAK010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552107"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VGT44_14125"
     CDS             <1..853
                     /locus_tag="VGT44_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2581988.1"
                     /translation="TMSLMILARVVFWQGDLVRAQTLAEKGLASASETGTTSAEALAL
                     ALHGEIALAQGETTTARLLIERSHTIWQQVGNQGMLASTRALLAKVLAVEGDHTAART
                     MYEESLLGGLGIVDIAPTLEGLAVVVAEQGETTWAVRLLAAAAALRDSLGAPLPPVCR
                     ADYERSVAAARAQLGEQAFAVAWAEGYGITWEQALAARGPVTIPIETHLPPPAKSPPT
                     LPFGLTAREVEVLRLLAQGLTDAQIAEQLIISPRTVNTHLKAIYGKMQVSSRVAATRH
                     AIDQHLI"
     gene            1111..1680
                     /locus_tag="VGT44_14130"
     CDS             1111..1680
                     /locus_tag="VGT44_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV2581989.1"
                     /translation="MRKVIVSEYVTLDGVMEDPGGAEGFKHGGWSFGFGGAEQQQYKF
                     EELFACDALLLGRRTYEGFAAAWPNMPGTGAYGERMNSLPKYVASTTLSEVAWNATLI
                     KGDLAEELSRLKQGTGQDILIFGSGELVHTLHERDLIDEYRLMIFPIVLGSGKCLFPG
                     GNEKKVLKLVETRTLGSGVVLLTYRPDKP"
     gene            complement(1639..1779)
                     /locus_tag="VGT44_14135"
     CDS             complement(1639..1779)
                     /locus_tag="VGT44_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581990.1"
                     /translation="MSIHRETSSVLQSVVARRAVGAFGEPPGAIRPRLWFVGPVGEQH
                     DA"
BASE COUNT          461 a          606 c          636 g          424 t
ORIGIN      
        1 gacgatgtcg ctcatgatcc tggcacgtgt ggtgttttgg caaggtgatc tcgtgcgggc
       61 gcagacctta gcagagaagg gtctcgcgag cgcttcggaa acgggcacca cgagtgctga
      121 agcgcttgcg ctcgctctcc atggggaaat cgccctcgct caaggggaga cgacgaccgc
      181 gcgcctgttg attgaacgaa gccacacaat ttggcagcag gtcgggaatc agggaatgct
      241 cgcctcgacg cgcgcgcttc tcgcaaaggt cctcgccgtg gagggtgacc atacagcagc
      301 acgtacgatg tatgaagaga gcctgcttgg aggtctgggc atcgtagata ttgctcctac
      361 gctcgaaggt ctggctgttg tggtcgcgga gcagggtgag acgacatggg cagtgcggct
      421 cctggccgca gcagcagccc tgcgcgactc cctcggcgcg cccctaccac ccgtctgccg
      481 tgccgactac gagcgctccg tcgctgccgc acgtgcccaa ctgggtgagc aagcctttgc
      541 ggtggcgtgg gccgagggat atggcataac atgggagcaa gcccttgcgg ctcgaggacc
      601 agtgacgatc cctatcgaga cacacctgcc tccaccagcg aaatcacctc ccaccttgcc
      661 tttcgggctg acggcgcgcg aagtggaggt gttgcgcctg ctggcgcagg gactgactga
      721 cgcccagatt gccgagcaac tcatcatcag tccgcgcacg gtcaacaccc atctgaaggc
      781 gatctatggg aaaatgcagg tctcttctag agtagctgcc acacgccatg cgatcgacca
      841 gcacctcatt taacgcgcca atcctcgcca tccatgcagc ctgagcatcg tctacctgat
      901 tctacgtaga actccttgtt cccattcgcc tgctgctcta tgaagatacg cagccaagac
      961 accttgttca agggatgtgt tctggaccgc cctcacgtat actcttacta aagcaatggg
     1021 tgcgcggtct ctctcattgg gagatcctga tggacatcgc gtattggcac attgcaagca
     1081 ccatcgtatc gacgaaagaa ggagaacacc atgagaaaag tcattgtttc cgaatatgtg
     1141 acactggacg gcgttatgga agatcccggc ggcgcagaag ggttcaaaca cggcggttgg
     1201 agttttggat ttgggggtgc agaacagcag cagtataagt tcgaggaact cttcgcttgc
     1261 gatgcgcttc tgctgggccg ccggacctat gaagggtttg ccgccgcctg gccgaacatg
     1321 cctgggaccg gggcgtatgg tgagaggatg aacagcctgc ccaaatacgt tgcttcgacg
     1381 accctctctg aagtggcatg gaatgccacc ctgatcaagg gcgatctggc ggaagaactg
     1441 tccagactca agcaggggac tggtcaggat atattgattt tcggcagtgg cgaactggtc
     1501 cacacactgc acgagcgcga cctcatcgat gaatatcgcc tcatgatctt tccgattgtg
     1561 ttgggaagcg ggaaatgtct gttcccaggc gggaacgaga agaaagtgct caaactggtc
     1621 gagaccagga cattgggttc aggcgtcgtg ctgctcacct accggcccga caaaccataa
     1681 acggggccgg atagcgccgg gcggctcgcc gaacgcgcca accgctcgcc gcgccacgac
     1741 gctctggagc acagaggagg tctctcgatg aatactcatc tctttgctcc agtcatttta
     1801 gcctggccgc ctccctgtgc tggggcagtg gcgattttcg tggggtgcta gcctcccgcc
     1861 gcaccaacgc ctcataccaa ctcgcgtccg gtaataaaat caaggaggtg agcatgcaaa
     1921 agagggagag caaaggaata tcagaaagga tatgagtaga catgaccacg cagaatcatc
     1981 caagcacgcc cgttgagagc ggctatgttc ccgtgaacgg tctggagatg tactacgaga
     2041 tccacggcag cggacggccg ctggtgctgc tgcacggcaa tctctcgacc attggcagct
     2101 cgttcggcaa ggtgctcccg acgcttt
//
LOCUS       DASZAK010000800         3736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_552133, whole genome shotgun sequence.
ACCESSION   DASZAK010000800 DASZAK010000000
VERSION     DASZAK010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552133"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2715
                     /locus_tag="VGT44_14140"
     CDS             <1..2715
                     /locus_tag="VGT44_14140"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEV2581991.1"
                     /translation="GLMTRGDALRRMDRDQAALPFLDAAGLEFLAIGDEVSWARTRIG
                     RVSACLQLNRTSEALIDAAAAREVFVRYGKLLRAGMIDVNAAIINYESGQYDQALRLF
                     DRAIETYALQGEGVELFIARAKGNKAIILAAMGRFREAVALHEQARATFASHEGQEVS
                     VAREELNIAQIYAAQGHYSHALILFDRSRAIFQRHKMQFQAAEVAQQTCLCLLRMNRT
                     REAYELAGETVNYFRQSPGNRHNLAHSLMYQAEAATLEGDYRDADEKLQEASIHLEEI
                     GFTGLASIVRLHQAELYFADGKIEDSLREARHVADAFAEQEALPQLARAALLQARIAE
                     NLGDTASAQYLCDQALDIAQGQDLLDLKYRCDDLLGQIAERHDDLEAAARYYDRAING
                     IDVVQSHLVMDERSSFLENKGGVYKRAVILALKRGSNERALVYVEKAKSRVLGDYLRN
                     NIDIRLRAGDQAGEVILEDLARLREEQAWFSNIVYEAQNESSLSDTAVLRIRSMAPGQ
                     ARQEMQKRERNIEQMLEQLQLRLAGDLVSRSRPQWTDSIITKLWPKIEHAALMLEYYL
                     TEQDLYIFQLTRDGIAVHVVKDAVPRLERLISLWRVNLDLAAQASGARDRAQAFAGLQ
                     ENSLGLLQRLYDLLLRPVSSALGSCEHLTVVPYGMLHYVPFHCLFDGVQFLIEQLDVS
                     YLPAAALMEICHQRGQRAEANRIPLKKSLVMGLTDGGRLAFAMQEAEAVAKQLGAPCI
                     LNQDATTSLLREVGASSPIVHIAAHGLSRLDAPNFSYIKLADRQLSTIEVFNLDLSSC
                     TLVTLSACETGRAIVGGLDEVIGLGRGFLYAGAASLLPTLWKVDDASSAELMEMFYQA
                     LLSDYGKAAALAGAQRAFLARARTSILPYRVHPYFWAGFHLIGDPGPL"
     gene            2791..>3736
                     /locus_tag="VGT44_14145"
     CDS             2791..>3736
                     /locus_tag="VGT44_14145"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF006681.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEV2581992.1"
                     /translation="MVSISDDGYFVSIKERDVDAQSVAPSDISILPGIALLPGFVNTH
                     SHVFQRALRGHTHRPLSKQDTFWTWRHAMYAEAQRLNPELLYAGALMTYREMLAAGYT
                     SVGEFHYVHHQPGGQPYADPNVMAKAVIQAGHDAGIRVVLLMTAYAQSGFNQPPEEDQ
                     LRFCDGSVDAYLHRVEALRVTGLPIGVAPHSVRAVPEEWFRAIADYSHRHRLPLHVHA
                     DEQRAEVEQCQAAYGCTPIELLERVGALGTMTTIVHATHANATEIRLLAQNGCTVCVC
                     PTTEGDLGDGIAAYAELLAAQIPLAIGSDSNTRLDPIEE"
BASE COUNT          828 a          971 c         1068 g          869 t
ORIGIN      
        1 ggcttgatga cccgtggcga tgctttgcgg cgtatggacc gcgaccaggc tgctcttccg
       61 tttttagatg cagccggact cgaatttctt gcaattggcg acgaagtgag ctgggcgcgc
      121 acacggattg gtcgtgtcag tgcctgcttg caactgaacc gcacgagcga agccttaatc
      181 gatgccgcag ctgctcgtga agtctttgtg cgctatggaa agctcctgcg ggccgggatg
      241 atcgatgtca atgccgcgat aatcaactac gaatcggggc agtatgacca ggcattgcgg
      301 ctgttcgacc gtgctattga aacatacgct ttacagggcg agggggttga actctttatt
      361 gcccgtgcta agggcaataa agccattatc ctggcggcta tgggcaggtt tcgcgaagca
      421 gtagctctac acgaacaggc gcgggccacc tttgccagtc acgagggaca ggaggtttcc
      481 gtagcgcgag aggaattgaa tatcgcccag atttacgcgg cgcagggaca ctatagccac
      541 gcgctgatac tcttcgatcg cagccgtgct atctttcaac gtcacaagat gcaatttcaa
      601 gcggctgagg tcgcccagca aacatgtctg tgcctgctac ggatgaatcg cacgcgggag
      661 gcatacgaac ttgccggtga aacggtgaac tacttccgcc aatcaccggg gaaccggcat
      721 aatcttgctc actcactgat gtaccaggct gaggctgcca cgctcgaagg ggattatcga
      781 gatgccgatg agaaattgca agaggccagt atacacctgg aagagatcgg ctttacaggc
      841 cttgcttcga ttgtgcggct gcaccaggct gagctatact tcgccgatgg gaagatagag
      901 gacagtttgc gcgaagctcg tcatgtcgcg gatgccttcg ctgagcagga ggcgctgcca
      961 caactggcac gagcagcatt gctacaagcg cgtattgctg aaaaccttgg cgatacagcg
     1021 agcgcacagt atctttgtga tcaagctctg gatattgcgc aaggccagga tttacttgac
     1081 ctcaagtatc gttgtgatga tctgctggga caaattgctg agcgtcatga cgacctggag
     1141 gcggcagcac gttactacga tcgcgcgatc aatggtatcg acgtggtaca aagtcacctc
     1201 gtgatggatg agcgcagcag ttttctggaa aataagggtg gagtgtacaa acgagcagtg
     1261 atcctggcgt taaaacgtgg aagcaacgaa cgggcccttg tctatgttga aaaagccaag
     1321 tcgcgggtgt tgggcgatta cctgcgcaat aacatcgata ttcgtttgcg tgcaggtgat
     1381 caagcgggag aggtaatcct cgaagacctg gcacgactac gcgaggagca ggcgtggttc
     1441 agtaatattg tgtacgaggc gcagaacgaa tcgagcttga gcgatacagc ggtattgcgc
     1501 atacggtcga tggcgcctgg tcaagcgcgg caggagatgc agaagcgcga gcgtaacatc
     1561 gagcagatgc tagagcaatt gcagttgcgc ctggcaggtg atcttgtgtc caggtcgcgc
     1621 ccacaatgga cggatagtat catcaccaaa ctttggccaa aaattgagca cgcggccttg
     1681 atgttggagt attatcttac cgagcaagac ctgtacattt tccaacttac gcgtgatggt
     1741 atagctgttc atgtcgttaa agacgcggta cccaggctgg aacgtctcat ctcgctctgg
     1801 cgtgtcaacc tggatctggc tgcgcaggcg agtggcgcga gggatcgcgc tcaggccttt
     1861 gccgggttgc aggagaatag tcttggcctg ctgcaacgtc tctatgatct gctgttgagg
     1921 cctgtatcta gcgcgttggg tagctgcgaa cacctgacgg ttgttcccta cggtatgttg
     1981 cattatgtac cgttccattg tctttttgat ggcgtgcagt ttctcattga gcaactggac
     2041 gtgtcctatt tgcctgctgc cgcgctcatg gaaatttgtc atcagcgcgg acagcgtgcc
     2101 gaggcaaaca ggataccgtt gaaaaagtcg cttgtgatgg ggcttaccga cggtggccgc
     2161 ctggcctttg cgatgcagga ggccgaagcg gttgcaaaac aattgggcgc gccatgcatt
     2221 ctcaaccagg atgctacgac ctcgctgctg cgcgaggtcg gcgctagcag tcccatcgtg
     2281 catattgctg cgcatggtct ttcgcggctg gatgctccca acttctcgta tatcaagctt
     2341 gccgaccgcc aattgagtac catcgaggtc ttcaatctcg atctctcctc gtgtactctc
     2401 gtaacgttaa gcgcctgcga aactgggcgc gcaattgtag gcggcctgga tgaggtgatc
     2461 ggcctgggac ggggtttcct ctatgctggc gcggcttcgc tgctgcctac actttggaaa
     2521 gttgacgacg ccagcagcgc ggaactcatg gaaatgttct accaggcgtt attgagtgat
     2581 tatggcaaag ctgctgctct tgcaggtgca cagcgcgcgt ttctagcacg cgctcgcacc
     2641 tcaatcctcc cctatcgagt gcatccgtac ttttgggcgg gtttccattt gattggtgat
     2701 cctggtccat tgtgaaatcg ggaaaggatt tttatgcagt atctcgctcc agactatgtt
     2761 tgcacccctc aaggtttaca ggtcaacatg atggtctcaa tctcagatga tggatatttt
     2821 gtttccatta aagagcgaga tgtagacgcc caatccgttg ccccctctga cattagtata
     2881 ctcccaggaa tagctctcct ccctggcttc gtcaacaccc atagccatgt cttccagcgc
     2941 gccctgcgtg gacatacaca ccgcccgctc tccaaacaag acacgttctg gacatggaga
     3001 cacgccatgt atgccgaggc ccagcgactg aatcccgagc tcttgtatgc gggagcgttg
     3061 atgacctatc gagaaatgct ggcggctggc tacaccagcg tgggggagtt tcattatgtg
     3121 catcatcaac ctggcggaca accttatgct gatcccaacg tgatggccaa agcagtaata
     3181 caggctgggc atgacgcggg aatacgagtc gtcttgctca tgacggccta cgcgcagagc
     3241 ggttttaatc agccaccaga ggaagatcaa ctccgtttct gtgacggctc cgtcgatgct
     3301 tatcttcatc gtgttgaggc actgcgcgtc acaggtctcc ccattggtgt tgccccgcac
     3361 tccgtgcgag cggtgccaga agagtggttt cgcgctatcg ccgactatag ccatagacat
     3421 cgactcccat tgcatgtaca cgctgacgag caaagagctg aggtcgagca atgccaggca
     3481 gcgtatggct gtacgccgat tgagctacta gaacgtgtcg gcgcattggg aacgatgacg
     3541 acaatcgtgc atgccaccca cgccaatgct accgaaattc gcttactcgc gcaaaacggc
     3601 tgtacagtgt gtgtctgccc cacaacagag ggagacctcg gagacggtat tgctgcctat
     3661 gcagagcttt tggccgcaca aatccccctg gcaattggtt ctgacagcaa tacccgactt
     3721 gatcctatcg aggaac
//
LOCUS       DASZAK010000801         5643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_481732, whole genome shotgun sequence.
ACCESSION   DASZAK010000801 DASZAK010000000
VERSION     DASZAK010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481732"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3101)
                     /locus_tag="VGT44_14150"
     CDS             complement(<1..3101)
                     /locus_tag="VGT44_14150"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HEV2581993.1"
                     /translation="MKNKVTYRQQYTRCGKQRCRKCKEGPGHGPYWYAFYSEKGRTVS
                     KYIGIHAPADLNISQTSASAVRAQTAPAAPGNGFPAIVGATDQAEITPTNEATLEKEM
                     DGQPVLRIYVLGHFRIELRNGNEWVPVTNRTWQRRRARALLGCLLSNSGRRLGREQAM
                     EALWPDLDIDTAANRLNGAVHEVRQVLEPGLSRPAASRMLRLERDMLLLADASLIWVD
                     TEAFEKLLNKSNTLSSPAEVEQAIEEALLLYGGDYLLEELYSEWAAPRRESLRRSWMG
                     LLLKLAELRAGRGAYISAIEPLDRLLTSDPTHETAVRRLMELLTLLDRRGEAFQVYHR
                     LVETLRREYESEPLSETTALYRALRQGTYQIPRPVVLAATPAPALPEASQPAELSPDT
                     EPSPVFPRPALQLVRHNQSKLVGRDNELETMQQFLHAIESLSLEKKNRRKTAHFMLLL
                     GESGIGKTRLAEELSYEADQRGWSVVWAHGYEQEGTIPYRPWAEIFRALLKDIQPSQL
                     IKHLEARIRSSPTQDAPPSAGNGISPAQARLARINSLVPELGAFEVLRDTFSTTPEQE
                     RLHLWEAASAVLGILSRSAPLLLVLDDLHWTDDSSLALLAYVVRHMQYEHIAIVATAR
                     DGELAQESNLSTLMNDLRREQTVLTLPVKPLNEISIGSLVAHLPEYIVQDIQRQAGGN
                     PFFAEELARVSETSLADMNTPESIKAVLERRLSKLSSDCQVLLGKAAVLGGSFALSQL
                     IFLAGDQGANEESIFDLLEEALRAGLLTEEGTGARITYHFWHPLIVNHLYERQSAARR
                     GQLHRKAAHALIELHQGNPDDVAATITHHLVRGGSDITQVGQYAELAGSRAFSQYAFA
                     EAEYYYRLAVDAITRSGQTRQVEDPLHLASILERIAECNTVHGNFEELRRLYEQVLEL
                     RALHCSRSEGGVSMEEAQRQALIWREIGNAWSENGNMEEAMHCYEVGQQVMLDAGVMS
                     GAAWACLRQAHGNLNWRLGNNEIARRYAQEALEMLEPIMRQQEQEKQALPIAATELPS
                     RTAL"
     gene            3335..3877
                     /locus_tag="VGT44_14155"
     CDS             3335..3877
                     /locus_tag="VGT44_14155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581994.1"
                     /translation="MRAKRRTSIASVIGPWSILPIAILFVAGGASPLATQKAVIGPYN
                     VLLSFYSLPRAGQAMNATVESARPADALLFSNAVLNPAAGTDGSTIAVMITPDGETPH
                     VYDVQVTPPVRGKWLLHLQVTGPTGTVSGDIPIIVDGPPAIPIWLGWLIGLAPLPFIL
                     LFIAYQVTWRKRQMVRATRS"
     gene            complement(3864..4613)
                     /locus_tag="VGT44_14160"
     CDS             complement(3864..4613)
                     /locus_tag="VGT44_14160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2581995.1"
                     /translation="MQQHIHDVVQPVFSRKAASMLEALEQIANSATAQQLNKRINQFQ
                     SKIFNLSRDEQALLRERLAAVLPAILMEADARTLRMEAAGWLRLLVQAAYLVQPGSVF
                     RALVTAATHYPAIDKAECRAYLQMIVDCFWPFRHPYATYSWEQLPANATFLPLTKLFE
                     LNDDAIEESLMTVILQLPALDDRTLIDQVTPVALHWATHSDSGRRHRILPLLERISNP
                     DTRAALEHLCRDQDPVVRANAQRTVEMAKSV"
     gene            complement(4886..>5643)
                     /locus_tag="VGT44_14165"
     CDS             complement(4886..>5643)
                     /locus_tag="VGT44_14165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2581996.1"
                     /translation="VQDYFSRTAEGYVASFSHKAGSDLDRLIEIGEWDTSQRALDVAT
                     GGGHTALAVAPHVGQVTVTDLTPRMLAKAREFILAQGITNAVFQEADAENLPFAEGSF
                     ERVTCRIAPHHFPDMARFVREVARVLAPGGLFLLIDCMAPSDPELDIFDNRVEKWRDP
                     SHGRSCTVEEWQAFFAEAGLSIEHLEFFRKSHDYDDWTLRSQLPLDEKEQLARFILES
                     NVRIQRYFEVKRKANGQLESFTNDFILLKGRKS"
BASE COUNT         1105 a         1660 c         1599 g         1279 t
ORIGIN      
        1 agggcagtgc gggatggcag ctcggtagcg gcgatgggca gcgcctgctt ctcttgctct
       61 tgctgccgca tgatgggttc aagcatttcc agcgcttcct gtgcatagcg gcgcgcaatc
      121 tcgttgttgc caagccgcca gttgagattg ccatgcgcct gccgcaggca ggcccaggcc
      181 gcgccggaca ttacaccggc atccaacatg acctgttgtc cgacttcgta acaatgcatg
      241 gcctcttcca tgttgccatt ttcactccac gcattgccga tttcgcgcca gatcaatgcc
      301 tgccgctgtg cttcttccat actgacgccg ccctcgctgc gcgagcagtg cagggcgcgc
      361 aattcaagca cctgctcata gagacggcgc agttcctcga agttgccatg cacggtgttg
      421 cattcggcaa tgcgctccag gatgctggcc aggtgcagcg ggtcttcgac ctgccgcgtt
      481 tgcccgctgc gtgtgatggc atcgaccgcc aggcgatagt agtattccgc ctcggcaaag
      541 gcatactgcg agaaagccct ggagcctgcc agttccgcgt actgcccgac ctgcgtgata
      601 tcgcttccgc cgcgcaccag gtggtgggtg atggttgctg ccacatcgtc tggattgccc
      661 tggtgcagct cgatcaaggc atgagcggcc ttgcgatgca gctgcccgcg cctggctgcc
      721 gactggcgtt catagagatg attgacgatg agggggtgcc agaagtgata ggtaatgcgg
      781 gcgcctgtgc cttcttccgt aagcagcccg gcgcgcaacg cttcttcgag caagtcaaaa
      841 atactctcct cgttcgcgcc ctggtcgccc gcgagaaaga tgagctggct caaggcaaac
      901 gatccgccaa gtacggcagc tttaccgagc aagacctggc aatcgctgct caatttactc
      961 agtcggcgtt cgagtacagc cttgatcgat tccggcgtgt tcatgtcggc caggctcgtt
     1021 tcgctgacac gcgcgagttc ttcggcgaaa aatggattgc cacccgcctg gcgctggata
     1081 tcctgcacga tgtactcagg taagtgcgca accagcgagc cgattgatat ctcgttgagt
     1141 ggcttcacgg gcagcgtcag gacagtctgt tctctgcgca aatcattcat gagtgtggag
     1201 agattcgatt cctgggccaa ttctccatcg cgagcggttg cgacgatggc gatgtgttcg
     1261 tactgcatgt ggcgcaccac ataggctagc agggcaaggc tgctgtcgtc cgtccagtgc
     1321 aggtcgtcca gcaccagtag cagcggcgca gagcggctca agatgcccag gacggcggat
     1381 gccgcctccc ataggtgcag gcgctcctgt tctggcgtcg tcgagaacgt atcgcgcagt
     1441 acttcgaatg cgcccaattc cggcaccagg gagttgatgc gcgctaacct ggcctgcgcc
     1501 ggtgaaatcc cgttccctgc tgatggggga gcgtcttgcg tgggagatga ccggatccgc
     1561 gcctccaggt gtttgatgag ctgcgatggt tggatgtcct tgaggagcgc ccggaagatt
     1621 tccgcccagg ggcgataggg gatggtcccc tcctgttcgt acccatgcgc ccagacaaca
     1681 gaccagccgc gttgatcggc ctcgtagctc aactcttccg ccaggcgcgt ttttccaatg
     1741 ccggattctc ccagcagcag catgaaatgc gcggtcttgc gcctgttttt cttctccaac
     1801 gacagtgact cgatggcgtg taaaaactgc tgcatagttt caagctcatt gtctcgcccc
     1861 accagcttgc tctggttgtg gcgcacaagt tgcagcgccg gtcgtgggaa aaccggggat
     1921 ggttcagtat caggcgacaa ctcggcaggc tgagaggctt ccggcaatgc tggagctggc
     1981 gtggctgcca gcacgactgg ccgaggaatc tggtacgttc cttggcgcag ggcacggtac
     2041 aacgctgtcg tctcagagag tggttcgctc tcgtattcgc gacgcagcgt ctctaccagg
     2101 cgatgataca cctggaatgc ctcaccacgc cggtcaagca atgtcagcag ttccatcagg
     2161 cggcgcaccg ctgtttcatg tgttggatct gaggtgagca ggcgatcgag cggctcgatg
     2221 gcgctgatgt atgccccccg gccggcgcgt agttccgcga gcttcaagag taaccccatc
     2281 caactgcggc gcagcgactc ccggcgcggc gcagcccact cggaatagag ttcttccagc
     2341 aaataatcgc cgccgtagag caagagcgcc tcttctatcg cctgttcgac ttctgcgggg
     2401 ctagaaaggg tattgctttt gtttagcagc ttttcaaagg cttccgtatc gacccagatc
     2461 aaactggcgt cggccagaag cagcatgtcc cgttccagcc gcaacatacg tgatgcagcc
     2521 gggcggctca gccccggttc cagcacttgc cgcacctcgt gtaccgcgcc attgagccgg
     2581 tttgccgccg tgtcgatgtc caggtccggc cacaatgcct ccatcgcctg ctcgcgtccc
     2641 aatcgcctgc cgctgttgct gagcaagcag ccgagcagcg cccgcgctcg cctgcgctgc
     2701 caggtgcgat tcgtgacggg tacccactcg ttcccgttcc tcaactcgat gcgaaaatgc
     2761 cccagcacat aaatacgcag caccggctgg ccatccattt ctttttccaa cgtagcctcg
     2821 ttcgttgggg tgatttctgc ctggtctgtc gcgccaacga tggcggggaa tccgttgccg
     2881 ggagcggctg gtgccgtttg cgctctcacg gcagatgcgc tagtttgtga aatattcaaa
     2941 tcagcagggg catgaatgcc aatatatttg ctgacggtac gccctttctc gctgtagaag
     3001 gcataccagt aaggtccgtg tcccggtccc tctttgcatt ttcgacaacg ctgcttccca
     3061 cagcgtgtat attgctgccg gtacgtgact ttgtttttca tgcggaatta ccctgccccc
     3121 agagaatttc cgaaaggcga ccacttttca attcgccatc agtatcctca agtcgttaag
     3181 tcttacagaa gacaccttaa ttataaccca gacggttaac gatactcaaa tttttcacca
     3241 atatgtctct ctcgccggta aagacgcact tgacgctgct agtatttcat ggcacactaa
     3301 cgtcctgaat tacaccgttc aaaggagccg tcacttgcga gcaaagcgaa gaacatcaat
     3361 cgccagcgtg atcggtccgt ggtcgatact tcctatagcc attcttttcg tggctggggg
     3421 agcgtcgcct ttagcgaccc agaaggcggt gatcggtcct tataatgtgc tgctcagctt
     3481 ctatagtctc ccacgcgcgg gacaggccat gaacgcgaca gtagagtcgg cccgaccggc
     3541 tgatgcgcta ctattctcca atgccgtctt gaaccccgct gcgggtaccg atggcagtac
     3601 gatcgccgtt atgattacac cagatgggga aacgccgcat gtctacgatg tgcaggttac
     3661 gccgccggtg aggggaaagt ggctgctcca cctccaggtc acagggccga cgggtacggt
     3721 gagcggtgat attcctatca tcgtggatgg tccacccgcc atcccaatct ggctgggctg
     3781 gttgatcggc ctcgcgccgc tgcctttcat actgctgttc atcgcgtacc aggtgacctg
     3841 gcgcaagagg caaatggtcc gcgctacacg ctcttagcca tttcaaccgt gcgctgtgcg
     3901 ttggcgcgca cgacagggtc ctggtcgcgg caaagatgtt caagcgcggc gcgggtgtcg
     3961 gggttgctga tgcgctccag gaggggtaag atgcggtggc ggcgtcctga atcgctatgc
     4021 gttgcccaat gcagggcgac cggcgtgact tgatcgatga gggtgcgatc atcgagtgcg
     4081 ggcagttgca aaatgacggt catgagcgat tcctcaatcg cgtcatcatt cagttcgaag
     4141 agttttgtca gtgggagaaa tgtcgcgttc gcggggagtt gttcccacga gtaggtagca
     4201 tagggatggc ggaagggcca gaagcaatcc acgatcatct ggagataggc gcggcattcc
     4261 gccttgtcga tagcagggta atgtgtcgct gctgttacaa gcgcgcgaaa tacactgcct
     4321 ggctggacca ggtacgccgc ctgcaccagc agccgcagcc agccagccgc ctccatccgt
     4381 agcgtgcgcg cgtcggcctc catcaggatt gccggcagga cagcggccag ccgttcgcgc
     4441 agcagcgcct gctcgtctct agagagatta aagatcttgc tctgaaattg attgatgcgc
     4501 ttattcaact gctgcgctgt agcagaattt gcgatctgtt ccagcgcctc aagcatcgat
     4561 gctgccttgc gcgagaaaac tggttgcaca acatcgtgga tgtgttgctg catcgcagaa
     4621 gctgcatatt ccgtcgccag gagccgactc gatgagttca tttccaccat tcttccgcag
     4681 atcttcaact tgctcagaac tctcctattc tttgcaagca ggtttgtaac ggttgttact
     4741 atgataccaa gtatacctgt tctttgtgtt actgtcaaga aagatagcat cacactcgtc
     4801 gtagtacata gtcaaggtgc atttatttat acgccctaac tacagtataa ccagcgttac
     4861 aaaatggcta aacgcgccat tctcgtcaac ttttgcgccc tttcagcaga atgaagtcat
     4921 tggtgaagct ctccaattgc ccattcgcct tgcgtttcac ttcaaagtaa cgctggatgc
     4981 gtacattgct ctctaaaatg aaccgcgcca gttgctcttt ttcgtccaat gggagctgtg
     5041 agcgcagcgt ccagtcatca tagtcgtgtg atttgcgaaa gaattcgagg tgttcgatgc
     5101 tgagtccagc ctccgcgaaa aatgcctgcc attcttcaac cgtgcaggag cggccatgcg
     5161 atgggtcgcg ccacttttct acgcggttat cgaagatatc cagttctgga tcgctggggg
     5221 ccatgcagtc gatcagcagg aagagtccgc caggcgccag aacacgcgct acctcgcgca
     5281 caaaacgagc catatcagga aagtggtgtg gcgcgatacg acaggtcacc cgctcgaacg
     5341 agccttcggc gaacggcagg ttttcggcgt cggcttcctg aaataccgcg ttcgtgatgc
     5401 cctgcgccaa gataaattca cgcgccttcg ccagcattct cggcgtcaaa tccgttaccg
     5461 ttacctgtcc cacgtgtggc gcgacggcca gcgcggtatg cccgccgccc gtcgccacat
     5521 ccagcgcgcg ctgcgacgta tcccactctc ctatctcaat cagccggtcg aggtcgctgc
     5581 ccgccttatg cgagaagctg gccacgtatc cctcagcagt ccgcgagaaa taatcctgca
     5641 cct
//
LOCUS       DASZAK010000802         8660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_162565, whole genome shotgun sequence.
ACCESSION   DASZAK010000802 DASZAK010000000
VERSION     DASZAK010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8660
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162565"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1310)
                     /locus_tag="VGT44_14170"
     CDS             complement(75..1310)
                     /locus_tag="VGT44_14170"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV2581997.1"
                     /translation="MTTTSEPASTDRSAQQRLPLAGIRVLDIATMAAAPWAAAYLGEF
                     GADVIKVEQPRIGDPQRHWGTPKEGTGIFWKSISRNKRSITLNLGKPRGAEIFKRLVA
                     ETDVVIENFRPGTLERWGIGYDVLRDINPRLVMLRLTGFGQKGPYSARPGFGTLAESL
                     SGFAHLTGQPDGPPTLPNLPLADGLAGVTASFAIMLALYYRDARGGSGQMIDLSLCEP
                     LLRLIEPTLLDFDQLGVARGRIGNRSDHVAPRNTYLCADGRWIAISASAPSIFERLMV
                     AIGRPELRSDPRFSVNAARLAHIDELEAIINGWIGARGREEVLAALEQAQVAAAPIND
                     VPDIFANEHFQARESFMEITDPDLGTMRLVNVVPRLSETPGRVLTTGPALGQHNQEIY
                     HQLGLEDQDLEQLAGEGVI"
     gene            complement(1362..2270)
                     /locus_tag="VGT44_14175"
     CDS             complement(1362..2270)
                     /locus_tag="VGT44_14175"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HEV2581998.1"
                     /translation="MSVSLEWPTQVTVVDVTPRDGLQDADHFVTAEQKLSFIHVLLDA
                     GVQAIEATSFMHPKWIPQLADADYVASHLPRQAGVIYSALIPNLRGYERARAAQIDEV
                     VLVVSASESHNRANLNRSVDESLAQLREVAAQARRDGVLVRGAVATAFGCPFEGQVAP
                     GAVMRIVHAYADMDVQMVSLADTIGVGNPRQVFTLFQRARQELPAHITLGAHFHDREG
                     YGLANVFAALQAGVTTLDAAIGGLGGCPYAPGAPGNLSTERLVEFLEAMGFSTGISLE
                     QLAKAREQLMEDLARGEPVTTGHSIR"
     gene            complement(2273..3496)
                     /locus_tag="VGT44_14180"
     CDS             complement(2273..3496)
                     /locus_tag="VGT44_14180"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV2581999.1"
                     /translation="MEHAENKPPLAGVRVLELGSFVAAPLAARMLADFGAEVLKVEPP
                     RRGDELRGWGTMVPTRAGRISAWWLSQARNKRLITLDLHEAEGQELALRLVEQVDIVI
                     ENFRPGRLEGWNLGYERMEAVNPRVVLVRISGFGQTGPYRERAGYGNVGESMGGMRYV
                     TGFPDRPPVRVGVSMGDALAAQQAAFGAVMALRAAERDGHGQVVDVAITEAVFGLTEA
                     MLTEYKHAGVVRERMGNAMLRAAPSNVYQTKDGHWLAIGGNGENVFRRFAQAMRRPDL
                     LEDARFIDNIARVKHHKELDQIIDAWVGSLTLAEAQVILDEAGVPAGPVMSIADIAAD
                     QHFASRGMLAGIPDERIPGGLAYMPGIMPRLTQTPGAIRHTGGDLGADNQAVYGSLLG
                     LSTQELERLQAKGII"
     gene            complement(3483..4265)
                     /locus_tag="VGT44_14185"
     CDS             complement(3483..4265)
                     /locus_tag="VGT44_14185"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV2582000.1"
                     /translation="MNSIEVPDTKVGVLDKAMRILYAFPGGDSSLTPQQIAGTTDMPL
                     PTVYRLAQVLSEHGLLMKVGQRFRLGMTLLRLGSMVAEGLDVRTQSLPHMRWLQEQAG
                     ENAELYIRSNESRIPIEVVLSPQNLRVFVEIGTTVPLHVGASGKVLLAWLPESEQTQL
                     IQASAARYPKHPLYDVEALKKSLACVRESGWAVSIDERGEGLSAIAAPIFDVGKEVVG
                     AMAMVAPALRLSAEKQVDSIPLVCEAGRRASLDMGYIADGTR"
     gene            complement(4610..5632)
                     /locus_tag="VGT44_14190"
     CDS             complement(4610..5632)
                     /locus_tag="VGT44_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582001.1"
                     /translation="MAGRSYRRSTPAENFRVVIGAIPWRLLILVPMLLVIAFPFFLYG
                     TTAGQRMVPALTNYFYHLSDAPTPVATPYPTFPTNLPQIGALLYSVQAGDNCDEILTN
                     QMHMVDAGQIFSDVTPGTVKALDNAIGQDCHALQPGMVIALYPHYPLVAIGGVIFAIN
                     PLSPQQPLPTPLINVQQQVGIDCSGGCDLSVRIAAHTAVQLLVQTTLQVRVGSWVWAQ
                     AMLPRKQVAGFAAYPYADPAALINGMSLRACDFQVNATHDDNSLSCSQLTPNTILDDN
                     GAWLYSVTGPGSLDHWRYALARFHFPGGTRVLLWLSLDNNGNLVYRQGNPAYRYDNAT
                     HVYVPA"
     gene            5827..6213
                     /locus_tag="VGT44_14195"
     CDS             5827..6213
                     /locus_tag="VGT44_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2582002.1"
                     /translation="MNYLAKILIIDDSPTLCLLMSRTLKKAGYDVITASNGQEGLRMA
                     LRERPHCIILDVVLPGISGFGLCRQLRALDPHRRVSIIMVSTKNTPLDQQWGLRQGAD
                     RYLPKPFTDEKLAQTVEEVLQAHSLR"
     gene            6368..7504
                     /gene="tal"
                     /locus_tag="VGT44_14200"
     CDS             6368..7504
                     /gene="tal"
                     /locus_tag="VGT44_14200"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEV2582003.1"
                     /translation="MTNPLLQLKAHGQSVWYDNIDRAQLVSGQFKRLLDEDGVVGVTA
                     NPTIFQKSISSGTAYDEQITQLIKEGSDTNSIYEALVLRDIQTVADLLRPIYDRSGMK
                     DGYVSLEVSPDLAHDTEKTIAEVRRFWKLVNRPNLMIKIPATQEGVPAVQMALTEGIN
                     VNITLIFALDNYRQVAEAYLSALEERNAEGKDISHIASVASFFVSRVDTLVDKLLEDK
                     AKASADSAQRFRSLEGKAAIANARLVYQEFKRIFHSPRFETLKHSGAHVQRPLWASTS
                     TKNPAYRDVLYAEELIGPDTVDTMPLETIVNFRDHGIANLTIENNIAQAQAELDALEQ
                     VGIHYHAVTQQLQDEGVQKFADSFHELFQGIAAKQKSIQEQVAR"
     gene            7596..>8660
                     /gene="zwf"
                     /locus_tag="VGT44_14205"
     CDS             7596..>8660
                     /gene="zwf"
                     /locus_tag="VGT44_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV2582004.1"
                     /translation="MPQRSVVEAPNPLREGIRMKSGPEPCVMVIFGATGDLTHRKLLP
                     ALYNLALEHPLPAGFSVVGFARRPYTDEQFQQQAMESINEYSRQKPVNQQVWESFATG
                     IRYLQADFHDPKGYERLGVLLNKLDAERGTGGNRIFYLSTPPSQYPEIIQNIGAAGLN
                     KSRKGWTRIIIEKPFGHDLASAKELNRQVSRVFKEEQVYRIDHYLGKETVQNILVFRL
                     SNGIFEPVWNRRYVDHVQIMVAENIGLEGRGGYYEESGAIRDMVQNHVLQLLSLVAME
                     PPITFEANAVRDEKVKVLHALQPLTGHDVVTNIIRGQYGPGWVNGHQVVGYREEPGVS
                     PISTTETYVAMKVFIDSWRWA"
BASE COUNT         1849 a         2635 c         2524 g         1652 t
ORIGIN      
        1 attgcgccca gggaaggtgc gtgaccggga acgagggcgc aattcattgc gcccctacac
       61 gccactagcc ggaattagat cacgccttcc ccggcaagtt gctcaagatc ttgatcttct
      121 aacccaagct gatgatagat ctcctggtta tgctggccga gggctggccc ggtggtcagc
      181 acacgtccgg gcgtttcact gaggcgcggg acgacattca ccaggcgcat cgttccaaga
      241 tcggggtcgg tgatttccat aaagctctcg cgagcctgaa agtgttcatt ggcaaaaatg
      301 tcgggaacgt cgttgatcgg cgcagccgct acctgggcct gctcaagcgc cgcaagcact
      361 tcctctcgcc cgcgcgctcc gatccacccg ttgatgatgg cctcaagctc atcgatgtgc
      421 gccaggcgcg cagcgttcac gctgaaacga ggatcgctgc gcagttcggg acgcccgatg
      481 gcgaccatca gccgctcgaa gatggagggc gcgctggctg aaatcgcgat ccaacggcca
      541 tcagcgcata gataggtatt tctgggagcc acgtgatcgc tgcggttgcc aatcctgccc
      601 cgcgcaacgc ccaactggtc gaagtcgagc aaggtcggct cgatcagccg gagcaacggt
      661 tcgcacagcg acaggtcgat catctggccg cttccaccgc gcgcatcgcg gtaatacaac
      721 gcgagcatga tggcaaacga ggccgtgacg cccgccagcc catcggccag gggcaaattg
      781 ggaagcgtcg gcggaccgtc gggctggccg gtgagatgcg caaagccgga gagcgactcc
      841 gccagcgtgc cgaagccggg gcgcgcgcta tacgggcctt tttgcccgaa ccccgtcagg
      901 cgcagcatga ccagcctggg gttaatgtca cgcagcacgt catagccaat tccccaacgc
      961 tccagcgtgc cagggcgaaa attctcgatc acgacatccg tctccgccac cagccgcttg
     1021 aagatttccg cccctcgcgg cttgcccagg ttcagcgtga tcgagcgctt attgcgggaa
     1081 atcgacttcc agaagatacc ggtcccttct ttgggcgttc cccaatggcg ctgcggatcg
     1141 ccaatgcggg gctgctccac tttgatcaca tcagcgccaa actcgcccag gtaggcagcg
     1201 gcccagggag ccgccgccat tgtagcgata tccagcacgc ggataccggc aagtgggagc
     1261 cgctgctgag cagagcgatc ggtggaagcg ggttcgctgg ttgtagtcat attacttgcg
     1321 cctttgctaa gtcatggtat aaaaacgaca accaccctct actatctaat agagtgtccc
     1381 gttgtgacgg gttcgccgcg agcgagatcc tccattaatt gctcgcgagc cttcgccaat
     1441 tgttcgaggg agatgccggt cgagaatccc atcgcctcca ggaattccac cagccgctcg
     1501 gtcgagaggt tgccgggcgc gccgggggcg tagggacagc cgcccagccc gccgatggcg
     1561 gcatcgagag tggtgacgcc cgcctgcagc gcggcgaaga cgttggcgag gccgtagccc
     1621 tcgcggtcgt ggaaatgcgc tcccagcgtg atgtgcgccg gaagctcctg gcgcgcgcgc
     1681 tggaacaggg tgaagacctg gcgcgggttg ccgacgccga tagtatcggc cagcgagacc
     1741 atctgcacat ccatgtcggc gtaggcgtgg acgatgcgca tgaccgcgcc tggcgcaact
     1801 tgcccctcga aaggacagcc gaacgccgtg gcgaccgcgc cgcgcaccag caccccatcg
     1861 cgccgcgcct gcgctgccac ctcgcgcaac tgcgccagcg actcatcgac cgaacggttg
     1921 aggttcgcgc ggttgtgcga ttcgcttgca gagacgacca gcacaacctc gtcaatctgc
     1981 gccgcccgcg cgcgttcgta gccacgcaga ttggggatca gagcgctgta tatgacacca
     2041 gcctggcgcg gcaggtgcga cgcgacataa tcggcgtcgg cgagctgtgg aatccacttc
     2101 gggtgcataa acgaagtcgc ttcgatagcc tgcacaccgg cgtccagcag gacgtgtata
     2161 aagctcagtt tctgctcagc cgtcacgaaa tgatccgcgt cctgcagccc atcgcgcggg
     2221 gtgacatcga caaccgtgac ctgtgttggc cactcaagag atacgctcat cgttaaatga
     2281 ttcctttcgc ttgcaaacgc tctaactctt gtgtactaag tcccagcagg ctgccgtaca
     2341 cagcctggtt atccgcgccc aggtcgccgc cggtgtgcct gatcgcgccc ggcgtctgcg
     2401 tgagacgcgg catgatacca ggcatatagg cgagaccccc cggtatgcgt tcatcgggta
     2461 tgcccgcaag cattccacgc gacgcgaagt gttgatcggc ggcgatatcg gcaatgctca
     2521 tgaccggccc ggcaggtacg cccgcttcat ccaggataac ctgcgcctcg gccagggtca
     2581 gcgacccaac ccaggcgtcg atgatctggt caagctcttt atgatgtttg acgcgggcaa
     2641 tgttatcgat aaaacgcgca tcttccagca ggtcgggtcg gcgcatggcc tgggcgaaac
     2701 gccggaagac attttcgccg ttgccgccga tggccagcca gtgtccgtct tttgtctgat
     2761 agacattcga tggggcagcg cgcagcatag cgttgcccat gcgttcgcgc acgactccgg
     2821 catgtttgta ttcggtcagc atggcctcgg tcaggccgaa aaccgcctcc gtaatcgcca
     2881 cgtctaccac ctgcccatgt ccgtcgcgct cggcggctcg cagcgccatg actgcgccga
     2941 acgccgcctg ctgcgccgcc agcgcatcgc ccatgctgac gccgacgcgc accggcgggc
     3001 ggtctggaaa gcccgtcaca tagcgcatcc ctcccatcga ctcgcccacg ttgccgtagc
     3061 ccgctcgctc gcgataggga ccggtctgcc cgaatccgga aatccgaacc agaacaactc
     3121 gcgggttgac ggcctccatg cgctcatagc caagattcca cccctccagg cggcctggac
     3181 ggaaattctc gatgacgata tcaacctgct ccaccagccg gagcgccagc tcctgccctt
     3241 ccgcttcatg caggtccagc gtaatcagcc gcttgttgcg cgcctgcgac agccaccacg
     3301 ccgatatgcg accagcgcgg gtcggaacca tcgtccccca tccgcgcaat tcatcccctc
     3361 ggcgcggcgg ctcgactttc agcacctccg cgccgaaatc ggccagcatc cgcgccgcga
     3421 gcggggccgc gacaaacgag ccaagctcca gcacgcgcac ccctgccagg ggcggtttgt
     3481 tctcagcgtg ttccatctgc aatgtacccc atatccagcg aagcgcggcg gcccgcctca
     3541 caaacgagcg gaatagagtc gacctgcttt tccgcgctca ggcgcaacgc cggcgcgacc
     3601 atcgccattg ccccaacgac ttctttgcca acgtcaaaaa taggcgccgc aattgccgaa
     3661 agcccctctc cacgttcatc aatactgaca gcccagccac tttcacgcac gcacgccagg
     3721 ctttttttca acgcctccac atcgtacagg gggtgcttgg ggtagcgagc cgcgctcgcc
     3781 tgtatcaatt gcgtctgctc gctctccggc agccaggcca gcagtacctt ccccgacgcg
     3841 ccaacatgca gcggaacggt cgtgccaatt tcgacaaaca ctcgtaaatt ctgcgggctg
     3901 agtacaacct caatcgggat gcgcgactcg ttggagcgga tatacagctc cgcgttttct
     3961 cccgcttgtt cctgcaacca tcgcatatga ggcagcgatt gcgtacggac atcaagcccc
     4021 tcggctacca tcgaccccag gcgcagcagg gtcataccca ggcgaaaccg ctggcctact
     4081 ttcatgagta agccatgttc gctcagcacc tgtgccagcc ggtagacggt tggcagcggc
     4141 atatcggtcg ttccggcaat ttgttggggt gtcagggaac tatccccacc tggaaaggcg
     4201 tagaggatgc gcatggcctt atcaagcacc ccaactttgg tatctggaac ttcaattgaa
     4261 ttcatagaac cctctcatgg cgttcacagc ttccagatat tccattattt ggaatactat
     4321 ccatatattg gaatatcgat gtatcaagca tacccgatac accgttgttt gtcaaccttg
     4381 ttccgattgg ttcacatgta tctatgagaa cagtgtacgt aaaaaagcgt aaacggcgca
     4441 tatacacgag tggtgtgcgt ataaaaagcg cgtaaacgtt tgtcgcctct tacgacgggt
     4501 ggctccctcc ctacaaagga cgagggcgac catgaccgcg cataaggcgt agcgggaggt
     4561 ggagaccctt gcggtcgccc tcgtcttttg tttgatgaga tcgattacgt tacgctggta
     4621 cgtagacgtg cgtcgcgttg tcgtagcgat aggccgggtt gccctggcgg tagaccaggt
     4681 tgccgttgtt gtcgagggag agccagagca ggacgcgcgt cccgccggga aagtggaagc
     4741 gcgcaagggc gtagcgccag tggtcgagcg agccggggcc ggtgacgctg tagagccacg
     4801 cgccgttgtc gtcaaggatg gtgttgggcg tcagctggct gcacgagagg gagttgtcgt
     4861 cgtgggtcgc attgacctgg aaatcgcaag cgcgcaggga catgccattg atgagagcgg
     4921 caggatcggc gtagggatag gcggcgaaac cggccacctg cttgcggggc agcatggcct
     4981 gcgcccacac ccacgagccg acgcgcacct ggagcgtggt ctgcacgagc aactgcaccg
     5041 ctgtatgcgc ggcgatgcgc acggacaggt cgcaaccgcc gctgcaatcg atgccgacct
     5101 gctgctgcac attgatgagc ggcgtcggca gcggctgctg aggcgagagc gggttgatag
     5161 caaagatgac accgccgatg gcgacgagtg gatagtgtgg ataaagcgcg atgaccatgc
     5221 cgggctgcaa agcatgacag tcctggccga tagcgttatc caatgccttc acggtaccag
     5281 gggtgacatc gctgaaaatc tggcctgcgt cgaccatatg catctggttt gtgaggattt
     5341 catcgcagtt gtcgcccgcc tgcacgctgt agagcagcgc gccgatttgc ggcagattcg
     5401 ttgggaaggt tggatagggt gtggctacgg gcgtgggtgc gtccgaaagg tggtagaaat
     5461 agttcgtcag cgcaggtacc atgcgttgac ccgcggtggt gccgtacaga aaaaagggga
     5521 aggcaatcac cagcagcatc ggcaccagga tcagcagacg ccagggtatc gcgcctataa
     5581 cgacgcgaaa attctccgca ggagtactcc ttcgatacga acggcccgcc atgctctccc
     5641 cctatccaga tcaagtagtt gcaacgtcta gcattcatta taacggcgca ggaaagcatc
     5701 actacacgca tatggcccat tatgcctgtt caggcagctt gattgcctac atcgcggcaa
     5761 tctacggtat acttgctaga atagcacaat attataagga acgcatgaag aaaggcgccc
     5821 atattcgtga actatcttgc taaaatcctc ataatcgatg acagccctac gctctgtctc
     5881 ttgatgtcac gcacacttaa gaaggccggg tacgacgtga ttaccgcatc aaacgggcag
     5941 gagggcttgc gcatggccct gcgcgaacgc ccgcactgca taatcctgga tgttgttctc
     6001 cctggcatca gcgggtttgg cctctgccgc cagttgcgcg cgctcgatcc gcaccggcgc
     6061 gtctcgatca tcatggtcag caccaaaaat acgccactcg accagcagtg gggtctgcgc
     6121 cagggcgcgg atcgctacct gcctaagccc ttcacggatg aaaagctggc gcagaccgtc
     6181 gaagaggtct tgcaggcgca ttcgctgcgt taactcacca taaatatttg gccattacgc
     6241 ctgttacgtg ctatcatgga agaaacaacc actggcaatg tggagagcgt aacattctag
     6301 ctttgtttcc agaccatagc ataacgtacc tgttcgtcaa tctatgccaa aggagaacaa
     6361 cacaaccatg accaatccat tactacaact caaggcgcac ggacagagcg tctggtacga
     6421 caacatcgac cgggcacagc ttgtctcagg tcagttcaag cgcttgctcg atgaggatgg
     6481 cgttgttggt gtgacggcca acccgaccat ctttcaaaag tccatcagca gcggcactgc
     6541 ctatgatgag cagattaccc agcttataaa ggaaggcagc gatacgaact cgatctacga
     6601 ggcgctggtg ctgcgcgata tccagaccgt cgccgatctg ctgcgcccga tttacgaccg
     6661 cagtggcatg aaggatggct acgtcagcct ggaggtctcg cccgacctgg cgcatgatac
     6721 cgagaagacc atcgccgagg tgcggcgctt ctggaaattg gtcaatcgtc ctaacttgat
     6781 gattaagatt cccgccacgc aagaaggcgt tcccgccgtt cagatggcgc tgaccgaggg
     6841 catcaatgtt aacatcacgc tcattttcgc gctcgacaac tatcgccagg tggcagaggc
     6901 gtacttgagc gcgctcgaag aacgcaacgc cgagggcaaa gatatcagtc acatcgcctc
     6961 ggtcgccagc ttcttcgtca gccgcgtcga tacgctggtt gataagctgc tcgaagataa
     7021 agcaaaagcc agcgctgaca gcgcgcagag gttccgctcg ctggaaggta aggccgcaat
     7081 cgccaacgcg cgccttgtct accaggagtt caagcgcatc ttccactcgc cgcgtttcga
     7141 gacgctgaag cacagcggcg cgcacgtgca gcgtccactg tgggccagca ccagcacgaa
     7201 gaacccggcc tatcgcgatg tactctacgc cgaggaactg atcggaccgg acaccgtcga
     7261 taccatgccg cttgaaacca ttgtgaactt ccgcgatcat ggcattgcca acctcaccat
     7321 cgaaaataac atcgcgcagg cgcaggcgga acttgatgcg cttgagcaag tgggcattca
     7381 ctaccatgcc gtcacccagc aactccagga cgaaggcgta cagaagtttg cggattcctt
     7441 ccatgaactc ttccagggca ttgcagcgaa gcaaaagtcc attcaggagc aggtggcgcg
     7501 atagttggga gtttcagcgg ctgcgttttc gtcatgaaat cgggcgcggc cgctgagacg
     7561 tggtaacaag agatcaaacg aggagagaag cacacatgcc tcagcgcagc gtagtagagg
     7621 ctccaaatcc cctacgagaa ggcatccgca tgaagagcgg ccccgaacca tgcgtcatgg
     7681 tcatctttgg ggccaccggc gatctgaccc atcgaaaact gctgcccgcg ctctacaacc
     7741 ttgccctgga acacccgctt ccggcgggtt tttcagtggt cggcttcgcg cgacgcccct
     7801 ataccgatga gcagttccag cagcaggcca tggagtccat caacgagtat tcgcgtcaga
     7861 agcccgtcaa ccagcaggtg tgggagagct tcgccaccgg tattcgttac ctgcaagccg
     7921 atttccacga cccgaagggg tacgagcggc tcggcgtgct gctcaacaag ctcgatgcgg
     7981 agcgcggcac cggcggcaac cgtatctttt acctgtcaac gccgcccagt caataccccg
     8041 aaatcatcca gaacatcggc gcggcgggcc tgaacaagag tcgcaaggga tggacgcgca
     8101 tcatcatcga aaaacccttc ggacacgacc tggcctcggc aaaagaactg aaccggcagg
     8161 tttccagggt gttcaaggaa gagcaggtct atcgcatcga tcattatctg gggaaagaga
     8221 ccgtgcagaa tatcctggtc ttccgcctgt ccaatggcat cttcgagccg gtttggaatc
     8281 gccgctacgt tgaccacgtg cagattatgg tggcggaaaa tatcggctta gagggacgcg
     8341 gcggctacta cgaggagtcg ggcgcgattc gcgacatggt ccagaatcac gtgctacaac
     8401 tgctttcgct ggtggcgatg gaaccgccga ttacgttcga ggccaacgcg gtacgcgacg
     8461 agaaggtcaa agtgctgcat gccttgcagc cgctgaccgg tcatgacgta gtgacgaaca
     8521 tcatccgcgg gcagtacggg ccaggctggg tgaatggaca ccaggtcgtg ggttatcgcg
     8581 aggagccggg ggtttcgccc atttcgacga cagaaaccta cgtcgcgatg aaggtcttca
     8641 ttgatagctg gcgctgggca
//
LOCUS       DASZAK010000803         5936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_162594, whole genome shotgun sequence.
ACCESSION   DASZAK010000803 DASZAK010000000
VERSION     DASZAK010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162594"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VGT44_14210"
     CDS             complement(<1..1286)
                     /locus_tag="VGT44_14210"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEV2582005.1"
                     /translation="MSDLTYEQLQYKRLHFLGIGGRGMSAMAQIALRYGAQVTGCDKG
                     ESYTTKALEAEGIPIQIGHSADHLANVDAFIYVPAVTTFDPDTPEFVEAQKRGIPCMT
                     WQELLGKWAAGRCALSVSGVHGKGTTTTLLSYMLLEAGYDPSCFVGAVVPRFNSSFRL
                     GSSPYLVNEADEYNHNFWHVHPRIAVITSIEYEHPEFFAGLEAMLYAFEGLLRRADTQ
                     GDWPFPPTAVINAGNTGCRMLLERLGDWNGRIVTYAVENPSQPLTGAAARANYIAYDV
                     QEDAEQRETHFRVRIHGLDDKETFPAARTFRYTLPGIYNVENALAALAAAHAAGVSYD
                     SALATLERFEGVKRRFEVRHIGQLALDGATLDDIVLIDDYAHHPTAIALTLAAARQRF
                     PGRRVVLAYQPDMFTRTKLLFDGFVAAFQGADVVILT"
     gene            1489..2196
                     /locus_tag="VGT44_14215"
     CDS             1489..2196
                     /locus_tag="VGT44_14215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582006.1"
                     /translation="MAQDPSDLNKDVTDSVTAPFPGDAGTTASGEQEVSSDDRLIKKD
                     DFLLHEYDSLRTEILQGISTRYTIITVTLTAFGAAFVIQNTILPLLFPALALVMMNIY
                     IANSYGTRKVTEFVKKHIETQVTTDGTHAVTRSELSRIGWQAHRDDSKSEQELRNRYS
                     AGKAVFFVSSLAASIAGWLFNSQAASSAKVWPGFIVFSFIISMLLAIPAFFVSDDTLV
                     RLYEQRPSLEDKMPLTK"
     gene            complement(2186..3133)
                     /locus_tag="VGT44_14220"
     CDS             complement(2186..3133)
                     /locus_tag="VGT44_14220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2582007.1"
                     /translation="MKQLRPGVWEISHPFQGQEEIVGSYLLAGGDELAIVDPGPGSMV
                     ESLLASIREAGFDPQDVTHILATHVHLDHAGNAGTLVRLMPKARVYAHSLGAPHLLDT
                     TRVVASASRIFGDRMKLLWGEIEPTPYERLRVVEHGDTLNVAGRRLEVHYTPGHAIHH
                     VSFFDAHFGEAFVGDVAGARLQGVPYVRPPTPPPDLDLEAWSASIELLKNLRPDVLYI
                     GHYGAIRDIGPHLAELQTRLYAWAEYVLAAMRDGKTEAEITSMLIEQNRAELLCAAQG
                     DERALDRYEIATNYPMTVQGYMRYWRKKHPERLATVPTS"
     gene            complement(3155..4732)
                     /gene="pruA"
                     /locus_tag="VGT44_14225"
     CDS             complement(3155..4732)
                     /gene="pruA"
                     /locus_tag="VGT44_14225"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEV2582008.1"
                     /translation="MAKMMESAATRAQIPFTNEPLKDFSKAENRQAQQAALEQVKSEL
                     GQTYPLVIGGQKIFNDATFASINPSQPDQVIGYFSSATVEQAQAAVQAAATAFEQWRH
                     VAAQERAAYIFAAADLLKERRFYYNAWMIYEVGKSWAEADGDTAEAIDFLEFYAREML
                     RLAADQPLARIEGEENQLVYIPLGVGAVIPPWNFPGAIMAGMTSASIVAGNTVVLKPA
                     STSPMIAWQFVRILEDVGLPGGVVNFLTGSGSTIGDALIENPQVRYIAFTGSRDVGLR
                     INELAAKPRKGQRWLKRTILEMGGKDAVVVDETADLEAAASGIVASAFGFQGQKCSAG
                     SRAIIVEQVYDQVLQKVIEKTQQLSLGDVTKPETYMGPVIDANALKKITDYIEVGKQE
                     GRLVAGGGHHGPGYFIEPTVIADVDPHARIAQEEIFGPVLAIIKARDFDDALHIANDT
                     EYGLTGSLYSQVPQRIERAREDYHVGNLYFNRKSTGAMVGVHPFGGFNMSGTDSKAGG
                     PDYLLLFTQAKAISAKK"
     gene            5151..5807
                     /locus_tag="VGT44_14230"
     CDS             5151..5807
                     /locus_tag="VGT44_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582009.1"
                     /translation="MSWNPNQGQDPNQPGAYPSQPSQYGGPTNDPYAAQQGGYQQGYQ
                     PGQQPQYPPPQYQQQPPQYQQQPPYDQQQQYSGYQQPYGAPGGNPAYGQLGPTSMGMQ
                     ANVAAGVSYIVGWITGLIFFFGEKQNRFVRFHAMQSIILFGGLTVLDIVVNVLAGALV
                     FSVPGFSVLLFGLVYLVGIAGFVLWIILLINAFQGKYFKLPVIGDYAEKYANPTGTTY
                     "
BASE COUNT         1149 a         1627 c         1805 g         1355 t
ORIGIN      
        1 gtcaggatca ccacgtccgc gccctgaaag gccgcgacaa agccgtcgaa gagcagcttt
       61 gtgcgggtaa acatatcggg ctggtaggcc agcaccacgc ggcggccagg gaagcgctgg
      121 cgagccgccg ccagggtgag cgcgatggcg gtgggatggt gtgcgtaatc gtcgatcaac
      181 actatatcgt caagcgtggc gccatcgagc gcgagttgcc ctatgtgcct gacctcgaag
      241 cgccgcttga cgccctcgaa gcgttccaat gtcgcgagcg cagagtcgta ggagacacct
      301 gccgcgtgtg ctgccgccag ggctgccagg gcgttttcga cattatagat gcccggcagg
      361 gtgtaacgaa acgttctggc ggcggggaag gtttctttgt cgtccaatcc gtggatgcga
      421 acgcggaagt gcgtttcgcg ctgctctgcg tcctcctgca cgtcgtaggc gatatagttt
      481 gcgcgggctg ccgcgccggt cagtggctgt gaggggtttt cgaccgcgta tgtcacgatg
      541 cgcccgttcc agtcgcccag gcgttcgagc agcattcggc agccggtatt gcccgcgttg
      601 ataactgccg ttggcggaaa tggccagtcg ccctgcgtat cggcgcggcg tagaagtccc
      661 tcgaaggcgt agagcatggc ttctaagcct gcgaaaaatt cgggatgttc gtattctatc
      721 gaggtgatga cggcgatgcg cgggtggaca tgccagaaat tgtggttgta ttcgtcggcc
      781 tcattgacaa gataggggct gctgcccagc cggaagctgc tgttgaaacg cggcacgacc
      841 gcgccgacaa agcacgaggg gtcatatccg gcctccagca gcatatagga gagcagcgtg
      901 gtggtggtac ccttgccatg tacgccgctc acggagagcg cgcagcggcc agcagcccat
      961 ttgcccagca gctcctgcca ggtcatgcag gggatgccgc gcttttgcgc ttcgacgaat
     1021 tcgggggtgt cgggatcgaa ggtcgtcacg gctgggacgt agataaaggc atcgacgttg
     1081 gcgagatggt cggcgctgtg gccgatttgg atgggaatgc cttccgcttc cagcgccttc
     1141 gtcgtatagg attcgccctt gtcacagccg gttacttgcg cgccatagcg cagcgcgatt
     1201 tgcgccatcg ccgacatgcc gcgcccgccg atacctaaaa aatgcagtcg tttatattga
     1261 agctgttcat aggtcagatc gctcataatt tgtgttgtcc tttctgcttt gcgaccgggt
     1321 ggatgaagtc ttcatgattg gcttccatac taaactaaac gatcatttga tcgcgcgttg
     1381 ccattttctt gtgtattgca tctctgccgg ttttgcgtta gtatgcattc gacacatggc
     1441 gctcaatgta atgtcatctc gtactattgt attgcatgga ggaacgcaat ggctcaagac
     1501 ccatcagatc tgaacaaaga cgtgaccgat agcgtcaccg cgccctttcc aggcgacgca
     1561 ggcacaacgg cgtccggcga acaggaagtt tcctctgacg accggctgat taaaaaagac
     1621 gacttcctgc tgcatgaata cgactcgttg aggaccgaga tattgcaggg catttcaacc
     1681 aggtatacga tcatcaccgt cacgctgaca gcttttgggg cggcgttcgt catccagaat
     1741 accattctgc cgctgctctt tcctgccctg gcacttgtga tgatgaacat ctatatcgcg
     1801 aattcatacg gcacgagaaa agtaacggaa ttcgttaaaa agcatatcga gacacaggtg
     1861 acgacagatg gaacccatgc ggttacacga agcgaactgt ccaggattgg ctggcaggca
     1921 cacagggatg atagcaaatc cgagcaagag ctgagaaaca gatactcggc ggggaaagca
     1981 gtctttttcg taagcagcct cgcggcttcg atagcaggat ggcttttcaa cagccaggcc
     2041 gcctcgagcg caaaagtatg gccgggattt attgttttct cgttcataat ctccatgttg
     2101 cttgcgatac ccgccttttt tgtgagcgac gataccctgg tcaggttgta cgagcagaga
     2161 ccttccctgg aggataagat gcctctcacg aagtagggac agtggcgagg cgttcggggt
     2221 gcttcttgcg ccagtaacgc atgtagccct gtacggtcat gggatagttg gtggcgatct
     2281 cgtagcggtc gagggcgcgc tcgtcgccct gggcggcgca cagcagttct gctcggtttt
     2341 gctcgatcag catggaggtg atctcggcct ccgtcttgcc gtcgcgcatg gctgccagca
     2401 catattcggc ccaggcgtag aggcgtgtct gtagctctgc caggtgcggg ccgatatcac
     2461 gtatcgcgcc gtaatgaccg atgtagagga catccgggcg caagtttttc agcagttcga
     2521 tgctggccga ccaggcttcc aggtcaaggt cgggcggcgg cgtgggtggg cgcacgtagg
     2581 gaaccccttg caggcgcgcc ccggccacgt ctcctacgaa ggcttcgccg aagtgggcat
     2641 cgaagaagct gacatggtgt atggcatggc caggggtgta atgcacctcc aggcggcggc
     2701 ccgctacgtt gagggtgtcg ccatgttcga cgacgcgcag gcgttcgtag ggggtcggct
     2761 cgatctcgcc ccaaagcagc ttcatgcggt cgccgaagat gcgcgaggcg ctggccacca
     2821 cccttgtcgt gtcgagcagg tgaggcgcgc ccaggctgtg ggcgtagacg cgcgcctttg
     2881 gcatcaggcg taccagtgtg ccggcattgc ccgcgtgatc caggtgtaca tgtgttgcca
     2941 ggatatgcgt tacgtcctga gggtcaaagc ccgcctcgcg tatagaggcg agcagtgatt
     3001 ccaccatgct gcccggtcca ggatcgacga tggccagctc gtcgccgcct gcgagcaggt
     3061 atgagccgac gatctcttct tgcccctgga agggatgcga aatttcccag acgccgggcc
     3121 tgagttgctt cacacgtgac aatatatgtt ctccttattt cttcgctgat atggctttgg
     3181 cctgcgtgaa cagcagcagg tagtccggtc cgccggcttt tgagtcggtg ccggacatat
     3241 tgaagccgcc gaaaggatgg acgccgacca tcgcgccggt gcttttacgg ttgaagtata
     3301 ggttgccgac gtgatagtct tccctggcgc gttcgatgcg ctgcgggact tgcgagtaga
     3361 gcgagccggt cagcccgtac tcggtgtcgt tggcgatatg cagggcgtcg tcgaaatccc
     3421 tggctttgat gatggccagc acagggccga agatctcctc ctgggcgatg cgcgcgtgtg
     3481 ggtctacgtc ggcgatgacg gtcggctcga tgaaatagcc ggggccgtga tgcccgccgc
     3541 ccgcgacgag acgcccttcc tgcttgccta cctcgatgta gtcggtgatt ttcttcagcg
     3601 cgtttgcgtc gatgaccggc cccatatagg tttccggctt tgtcacgtcg ccaagtgaga
     3661 gctgctgtgt tttctcgatg accttttgca gcacctggtc gtaaacctgc tcaacgatga
     3721 tggcgcgcga accggcggaa catttctgtc cctggaagcc gaaggctgag gcgacgatgc
     3781 ccgacgcggc tgcctccagg tcggcggtct cgtctaccac tacggcgtcc ttgccgccca
     3841 tctccaggat agtgcgtttg agccagcgct gccccttgcg cggtttggcg gcgagttcgt
     3901 tgatgcgcag acccacgtcg cgcgagccgg tgaaggcgat gtagcgcacc tgcgggttct
     3961 cgatcagcgc gtcgccgatg gtgctgccgg agccggtcag gaagttgacg acgccgcccg
     4021 gcaaccccac atcttccagg atgcgcacga attgccacgc gatcatcggc gaggtgctgg
     4081 cgggtttaag gacaaccgta ttgcccgcga cgattgaggc gctggtcatg ccagccatga
     4141 tcgctccggg gaagttccag ggcgggatga ccgctcctac gcccaggggg atgtagacca
     4201 gctggttctc ttcgccctcg atgcgcgcca atggctgatc ggcagccagg cgcagcatct
     4261 cgcgcgcgta aaattcgagg aagtcgatcg cttctgccgt atccccatcg gcctctgccc
     4321 agcttttccc gacctcgtag atcatccagg cgttatagta gaaacgacgc tctttgagca
     4381 aatctgctgc cgcaaagata tacgcggcgc gttcttgtgc cgcaacatgt ctccactgtt
     4441 caaaggcagt ggcggcggcc tgtactgctg cctgggcctg ttcgacggtc gcgctcgaaa
     4501 aatagccgat cacctggtcg ggctgcgagg gattgatcga tgcgaaggtg gcatcgttga
     4561 aaatcttctg gccgccgatt accagcggat acgtttgccc aagctcgctt ttgacttgct
     4621 ctaatgccgc ctgctgcgcc tggcggttct ctgccttgct gaaatctttg agcggctcgt
     4681 tagtaaaggg tatttgtgcg cgagttgccg cgctttccat catttttgcc attcgggtac
     4741 actctcctcg tgtgctagga ctgctttgta cttttattgt atctcaaaga gggcgggaat
     4801 cgatagggga caccggatgt ggaggcattg tgtgcttact cacaggcgcc aaatgccgaa
     4861 ttttaccggg caagtgtgga attccgcaca gtatatctct tgtaatcagc atagacttgc
     4921 agtatagtag tggcgagcaa tcgcccggtg gtgtttggta tggttgttta tccttgtagg
     4981 gaggactcat tattaatgct gttgtttcag aatgaccggg gcgagtacac tgttggcggg
     5041 tgcttattgc ttcaaaggca atttctagtg ttggtggcac ttagtggcca tcagaaaagt
     5101 cagattctca ttgacgcatt ttcacgttta tgaggaaagg agtcattaca atgagttgga
     5161 atccgaatca aggccaggac cccaatcaac cgggtgcgta cccgagtcag ccaagtcagt
     5221 atggcggccc aacgaatgat ccctatgctg ctcaacaggg aggctatcaa caaggctacc
     5281 agccgggcca gcagccccaa tacccgccgc cccagtacca gcagcagccg cctcaatacc
     5341 aacagcagcc tccctacgac cagcaacaac agtacagtgg gtatcaacag ccgtatggcg
     5401 cgccgggtgg caacccggct tatggccagc ttggaccgac ctctatgggg atgcaggcca
     5461 atgtagctgc cggtgtgagc tatatcgtgg gatggatcac cggcctgatc ttctttttcg
     5521 gtgaaaagca gaatcgtttt gtgcgtttcc atgccatgca atccatcatt ctttttggtg
     5581 gcttgacggt gctggatata gtagtcaatg tactggcagg cgcgctggtc ttttccgttc
     5641 ctggtttctc tgtcctgctg ttcggcctgg tgtacctggt agggattgcg ggatttgtcc
     5701 tctggatcat cctgctaatc aatgccttcc agggaaagta cttcaagctg ccggtcatcg
     5761 gtgactatgc cgagaaatac gccaacccca ccgggacaac gtattaaatc gcgttttgcc
     5821 aggatgccat tgaacctttg cagtccgtag tgttccgtcc agggcgatgt ggcgtgtagg
     5881 ggcgcaattc attgcgccct cgtcccagtg aagcgccatc accagggcgc aatgaa
//
LOCUS       DASZAK010000804         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_20374, whole genome shotgun sequence.
ACCESSION   DASZAK010000804 DASZAK010000000
VERSION     DASZAK010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20374"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1770)
                     /locus_tag="VGT44_14235"
     CDS             complement(217..1770)
                     /locus_tag="VGT44_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV2582010.1"
                     /translation="MATKQDYFIPGLRESMNGTSAAVEQQLEALPSEEYVVKTMPKVA
                     GVGGLTATFVLIIFFLTNVPSAIAAGAGTFTFWIVGGITFFIPCVIATAQLGHMFPHE
                     GSLYNWTHRAFGGYWSFFVSFCAWFPCVLLMIVAADGVVGYIQGLNNNWLTQPYQQGL
                     VLIVLLIVAGFLATRRVATVLYLAKMTLVIAFAAVFLVGFAGVVWLLTGHAAVVSVKT
                     PCDFGFCWNPQSSALPYYTLGLFGFICQAFLGIEVPLNMGGEMTHKRVVTRHLLWGCI
                     LVLLGYFVASFGVLIVTGTSQSGIPFGLVTTVQKAMGGTAGNIVALLVMINFVVTPAI
                     YCYAYARLLMVGGIDKRLPARIARLNKHRVPVTAMVFETIVAVVLAGILYIVIPSFTT
                     ASNQLSLNTIVYNVVISASTLVWAISTAFLFIDLVKFYFNDRRAFRKQLIFPMPVLWI
                     SIVFGTATCALSIIGTLLYSLIPGLISNGQWWYIVGGITAVCIVVAAIGSMLASSEAA
                     WQDLSKEEG"
     gene            2041..>3645
                     /locus_tag="VGT44_14240"
     CDS             2041..>3645
                     /locus_tag="VGT44_14240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEV2582011.1"
                     /translation="MPRDLPVGNGQLLLNFDKSYNLRDIYWPHVGQELHTAGDISHTG
                     VWVEGRFAWLDSPDWQREMLYEPETLVTHVTLQHADLQIKLVVRDCVDFNRPIFLRQF
                     TVTNQAEHPREVRLFFHYDWHIWDAADANTVFYYPPVQALIAYKQQAYFLMNGQIGRG
                     ANAQIGISSWATGKKESNGSEGTWRDAEDGQLGRNPIAQGSVDGTLSLHLPDIPPGSE
                     AIAYHWLSVGFDLGAVTDLEAMIRERGPESFITRTRNYWHSWVNKDAQDFYDLPTELV
                     DLYKRSLLVLRTQIDSEGAIIAANDADVQHFSNDSYSYMWPRDGALVANALSHSGYSE
                     ITRGFYDFCSGVLTTSGYLLHKYNPDRSWGSSWQSWIDGDGNPQLPIQEDETALVIYS
                     LWQHYHRFSDIEFVAPHFRSLITAAADFMVSYREPHTRLPGPSYDLWEERRGILTFTT
                     ATVYAGLDAASKFSALFGEDELAATYRAAADEIKQAALRYLWDEERGHFLRMITVDKD
                     GAIHKDATLDSSLCGLFQFGMFAATDS"
BASE COUNT          859 a         1108 c          969 g          709 t
ORIGIN      
        1 acaagcacca gcgcgcctat gagggcataa cgcgcgctgg tctgtaagcg tgataagaag
       61 gtcgatttca tccctgatag ttctgttctt cgtgtgtttc gcggaataac gttccacgac
      121 tcgcacatat tatacagaga caaatgccat tcggcaacga gcgagccagg gcacccttgc
      181 ggtcgccctg gctcgctcgc gctcatgaga tgagcgctac ccctcctctt tgctcaaatc
      241 ctgccaggca gcctcgctgc tggcgagcat actgccgata gcagccacca cgatgcagac
      301 cgctgtaatg cctcccacga tgtaccacca ttggccattg ctgatcaggc cgggaatcag
      361 gctatagagc agcgtgccga tgatactcag cgcacaggta gcggtgccga agacgatcga
      421 tatccacagc actggcatgg ggaagatcag ttgtttgcgg aatgcccgcc ggtcgttgaa
      481 gtagaacttc accaggtcga tgaacaggaa ggccgtagaa atagcccaca ccagggtcga
      541 ggcggaaatt acaacattat agacaatggt gttcaaactt aactggttgc tcgcggtggt
      601 aaaggatggg atcacaatgt agaggatacc ggccagcacc actgcgacga tcgtctcaaa
      661 gaccatcgcc gtcaccggca cgcggtgctt gttgagtcgc gcgattctgg ccggcagccg
      721 cttatcgatg ccgccaacca tcaggaggcg tgcgtaggca tagcagtaaa tggcaggcgt
      781 cacgacgaag ttaatcatga ccagcagcgc gacgatattt cctgctgtgc cgcccatcgc
      841 tttctggacg gtcgtcacta acccgaaagg aataccgctc tgcgatgtac cggttacgat
      901 cagcacgccg aaggaagcga cgaagtagcc gagcagcaca aggatgcacc cccagagcaa
      961 atgccgcgtc acgaccctct tatgcgtcat ctcaccgccc atgttcagcg gcacctcgat
     1021 cccaagaaac gcctggcaga tgaagccgaa caggcccagt gtatagtagg gcagcgccga
     1081 tgactgggga ttccagcaaa acccaaaatc gcaaggtgtc ttgacgctga cgaccgctgc
     1141 atggccggtc agcagccaga caacgcccgc gaaaccgacc aggaaaaccg ccgcgaacgc
     1201 gatgaccagc gtcattttcg cgagatacaa aaccgtcgct acgcgccgcg tggcgagaaa
     1261 gccggctacg atcagcagca cgatcagcac aagcccttgc tggtacggtt gagtgagcca
     1321 gttgttattc aagccctgga tatatccaac caccccgtca gctgccacaa tcatcagcag
     1381 cacgcaggga aaccaggcgc agaaggacac gaaaaagctc cagtacccgc caaaggcgcg
     1441 gtgcgtccag ttgtacaacg agccttcgtg cgggaacata tgcccaagtt gggcggttgc
     1501 aatcacgcac gggataaaaa aggtgatacc gcccacaatc cagaacgtaa atgtcccggc
     1561 gcccgcggca atggcagaag gaacattggt gaggaagaaa ataatcaaca cgaacgttgc
     1621 cgtcaggccg cctacaccgg ccaccttcgg catcgtctta acaacatact cctccgaagg
     1681 gagcgcttcc agttgctgct ctaccgccgc ggacgtgcca ttcatagact cacgcagccc
     1741 tggaataaaa taatcttgct ttgtcgccat tgttcactct gcctctcgtg tcagcccttt
     1801 atatctattc gtgtacaatc tctagcctac cccagtgttc ctctcagtga aaggaacata
     1861 gcaagtgtac tatagagctt tgcaattgtc gttcgattcc cccctcacat aggagtattt
     1921 acctaattgt atcggcaaaa ccgacaaaaa cataattcag tagatatgtt ataatcatcc
     1981 tcaacaattc cagcccggtg aacgcgcgtg gcgacgacgc aagagaaacg aggatgtaat
     2041 atgccgagag atctgcctgt tggcaatggt caactcctgc tcaacttcga taaaagctat
     2101 aacctgcgcg atatctactg gccgcacgtt ggccaggaac tgcacaccgc cggtgacatc
     2161 tcccataccg gtgtctgggt cgaaggtcgc ttcgcctggc tcgattcgcc cgactggcag
     2221 cgcgagatgc tttatgaacc ggagacgctg gtcacacatg tcacgctcca acatgccgac
     2281 ctgcaaatca agctcgtcgt gcgcgattgc gtcgatttca accgcccgat cttcctgcgc
     2341 cagtttacgg tcacgaacca ggccgaacac ccgcgcgagg tgcgcctgtt cttccactac
     2401 gactggcata tctgggacgc ggcggacgcc aacacagtct tctactaccc acccgtgcag
     2461 gccctgatcg cttacaaaca gcaagcctat tttctaatga atgggcagat tggcagggga
     2521 gcgaacgcgc aaatcggcat ctcgagctgg gcaacgggca aaaaagagag caacggatcg
     2581 gaaggcacct ggcgcgatgc cgaggatggg cagcttggcc gtaatccgat tgcccaggga
     2641 tcggtcgatg gcactctctc actgcacctg ccagatattc cccccggttc cgaggcgatc
     2701 gcctatcatt ggttgagcgt cggtttcgat ctcggcgcgg taaccgatct tgaagcgatg
     2761 atacgcgagc gcgggccaga gtcgtttatt acgcgcacgc gcaactactg gcatagctgg
     2821 gtcaacaagg atgcgcagga tttctacgat ctgccaaccg agctggtcga tctctacaag
     2881 cgcagcctgc tggtgctgcg cacgcagatc gactcggaag gcgctattat cgccgccaat
     2941 gatgctgatg tccagcattt ctccaatgac agctactcct acatgtggcc gcgcgacggg
     3001 gcgctggtcg ccaatgccct gagccactcc ggttactccg agatcacacg cggcttttac
     3061 gatttttgca gcggtgtgct gaccacgagc ggctacctgc tgcacaaata caaccccgac
     3121 cgctcgtggg gcagcagctg gcaatcctgg atcgatggcg acggcaaccc gcaactgccc
     3181 atccaggaag acgaaaccgc gctggtgatc tacagcctgt ggcaacacta ccaccgtttc
     3241 tccgacatcg aattcgtcgc gcctcatttc cgcagcctga ttaccgctgc cgccgacttc
     3301 atggtttcct atcgcgaacc ccatacgcgc cttccaggtc cgtcgtatga cctgtgggaa
     3361 gaacggcgcg gcatcctgac cttcaccacc gcgaccgtct acgccggcct ggacgccgcc
     3421 tcgaaattct ccgccctctt cggcgaggac gagctggccg ccacctaccg tgctgccgct
     3481 gacgaaataa agcaggccgc gctgcgctac ctgtgggacg aagaacgcgg ccacttcctg
     3541 cgcatgatta ccgtggacaa agacggcgcc attcacaagg atgccacgct ggatagcagc
     3601 ctgtgcggcc tcttccagtt cggtatgttc gccgcgaccg actcg
//
LOCUS       DASZAK010000805         1729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_125970, whole genome shotgun sequence.
ACCESSION   DASZAK010000805 DASZAK010000000
VERSION     DASZAK010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125970"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VGT44_14245"
     CDS             complement(<1..210)
                     /locus_tag="VGT44_14245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582012.1"
                     /translation="MTARQSYLIVGNGIAGATAAEILRNEDAAADITVVADDPFPVYY
                     RPALKDYLAGKIREDNLWARPISFYQ"
     gene            complement(454..1509)
                     /locus_tag="VGT44_14250"
     CDS             complement(454..1509)
                     /locus_tag="VGT44_14250"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2582013.1"
                     /translation="MLSKRYARLFVLALGISLVLAMLAGCEAGTTAGGSSTTTRPQSK
                     VLLTYHRHTDRATAVAWSPDGKYVASGSLDKTVQVWSAATGKTQLIYHGQTEGILAVA
                     WSPNGRYIASSSLDNTIQVWDASTGARISTYRGHKLPAQTLSWSPDSRSLVSGSSDQT
                     AQVWVATTGKTILTYRGHSAPVTAVMWSSDGKRIASGSVDTTVQVWDATSGKTLLTFR
                     GHTKVVTSLAWSPDSTSIISGSFDKTVQVWDASTGKVRFIYDGDNSAAARLDSSKDVL
                     PDLINFVAWSHNGKRIAAVTQEYCGDAVVMTWDAQTGKHLMFYPDLTIYAIAWSPDDT
                     RFVSAIEITDVEIALAP"
BASE COUNT          392 a          494 c          466 g          377 t
ORIGIN      
        1 ctggtaaaaa ctgattggcc gcgcccagag attgtcctcg cgtatctttc ctgccaggta
       61 atccttgaga gcggggcgat aataaacggg gaaaggatca tcagcaacaa cggtgatatc
      121 tgcggcagca tcctcattcc gtaagatttc ggcagccgtt gcgccagcaa tgccattgcc
      181 gacgatcaaa taggattgtc tcgccgtcat cgtatcaccc ccctgtcgaa aaatatgatg
      241 gtgcagcggc caggttttaa ctgcccgttt gcaaaatcaa gctggagtgt aggcttacaa
      301 agacaatgac gctggaatcc gcacttaaac cttcaatccc acctacccac accatatgtg
      361 catcctcaca tctacaacgt gcctgttcat tgttcagtat atcatgatat tctcaaaaat
      421 accattgcat tttacagtga gcgcccctca tgttcacggc gccagcgcaa tctccacatc
      481 tgtgatctcg atagcgctta caaagcgtgt atcatcgggc gaccaggcaa tagcgtaaat
      541 cgtcaggtcc gggtagaaca tgaggtgttt cccggtttgc gcatcccagg tcatcacaac
      601 cgcgtcgccg cagtattcct gggtgacggc tgctatgcgc ttgccgttat gtgaccaggc
      661 gacgaagttg atcaggtccg gcagcacatc ttttgagctg tcaagtcgag cagctgcgct
      721 gttatcgcca tcatagataa agcgcacctt tccggtactg gcatcccaga cctgcaccgt
      781 tttgtcaaaa gagcctgaaa taatcgacgt actatccggc gaccaggcaa gagaggtcac
      841 caccttcgta tgcccacgga acgtcagcag cgttttgccg ctcgttgcgt cccagacctg
      901 caccgttgta tcgacggagc ctgatgcgat gcgcttgcca tctgacgacc acatgacggc
      961 ggtcacaggg gcggaatgcc cgcgataggt caggatagtt ttccccgtcg tcgccaccca
     1021 gacctgcgcg gtctggtcag atgagcctga gacgagtgag cggctatcgg gcgaccagct
     1081 gagcgtctgt gcaggcaact tgtggccgcg ataggtggag atgcgcgcgc ctgtggaggc
     1141 atcccagacc tgtatcgtgt tgtctaagga ggaagaggca atatagcgcc cattgggcga
     1201 ccaggccact gccaggattc cttctgtttg gccgtggtag atcagctgcg tcttgcctgt
     1261 cgcggcgctc cagacctgca cggtcttatc cagggagcca gaggcaacat atttgccgtc
     1321 gggtgaccag gcgactgctg tagcccggtc ggtatgccgg tggtaggtca ggaggacctt
     1381 cgattgcggg cgcgtagtgg tagagctgcc accagcagtc gtgcctgcct cgcagcctgc
     1441 gagcatcgcc agcaccaggg aaatgcctaa tgccaacacg aataggcggg cataacgttt
     1501 cgacaacatg tagaagtctc cttctgtaat acagagagag ctacgacgac ctctttcctc
     1561 gcaggaagag caactccgat caactaccag gcttgcctcg cgccctggct cgaaattgcc
     1621 gcaccttcat gagattgcca caggcccact ggcgacacca gcaccggctc agattatggc
     1681 tttgatcgat aaacacccag tgacagtcgg gattggcaca gaccttcaa
//
LOCUS       DASZAK010000806         2596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_195859, whole genome shotgun sequence.
ACCESSION   DASZAK010000806 DASZAK010000000
VERSION     DASZAK010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195859"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VGT44_14255"
     CDS             <1..595
                     /locus_tag="VGT44_14255"
                     /inference="COORDINATES: protein motif:HMM:NF014860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2582014.1"
                     /translation="SGLERLMQQGLANDRGRVLVNERMETNLPGIYGIGDLVHGAGLA
                     HVASTEGEVAAENALGHHVSMNYDVVPNPIYTFPEIAFVGLTEAQAREQDATVRVERF
                     PWAANGKAVGSANSEGFTKVILGKHNEILGAHIIGPDATNLITEYSLAMRGELTGDEL
                     IDTIHPHPTLSEGLREAVLAAEGRPIHIPPRQAARAR"
     gene            651..2000
                     /locus_tag="VGT44_14260"
     CDS             651..2000
                     /locus_tag="VGT44_14260"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2582015.1"
                     /translation="MMKDVPDIETNAVDVGQVTPKTVEPARPAVPTVQPRVVIVGAGF
                     GGLRAATALRDVPVHVTVIDRNNHHLFQPLLYQVATASLSPADICAPIRHVLKKQKNT
                     EVLMGEVTGVDVQEQRVFMGDQAVPYDYLILATGARDSYFGHNEWAKFAPGLKSIRQA
                     TEIRSRILWAFEQAEKETDEEKRQAFLTFVLVGAGPTGVEMAGSIAGLAHKTLTDEFQ
                     HINPADARIILVEAVPRILLAFPERLANTAKNALNQLGVEVRTNSPVEDVNDEGVVIG
                     GKLLPAKTVIWTAGVAASPAGTWLGAETDRGGRVKVSSDVSVPGHPTIFVLGDTAQFM
                     QDGKPLPGVAQVAMQQGSYVARVIADRVAGKEHNAAFRYFDKGNMATIGRFHGIVSIG
                     KLQFAGILAWFAWLVLHLMFLIGFRNRLVVMFLWVWYYTTFQRGARLITSEDTSRSV"
     gene            2083..>2596
                     /locus_tag="VGT44_14265"
     CDS             2083..>2596
                     /locus_tag="VGT44_14265"
                     /inference="COORDINATES: protein motif:HMM:NF018827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582016.1"
                     /translation="MAERSMSEQTPFETWSKLIENTMKDNPALQRNSGQAVESGKDPW
                     ITLIDKLWQANPSSKLLPIDPAEITHAFQQIWTDALRHPDRTWANYADFVQSYTQMMT
                     GAALKFWGQGKDVEPTVSPEKGDKRFSTPDWQQNPAFDALKQSYLLASTTLLKSAAEV
                     QGLDEHQQRKL"
BASE COUNT          594 a          696 c          772 g          534 t
ORIGIN      
        1 gagcggactg gagcggctca tgcagcaggg gctcgccaac gatcgggggc gcgtgctggt
       61 caacgaacgc atggagacga acctgcccgg catctatggc atcggcgacc ttgtgcatgg
      121 tgcgggcctg gcccatgtcg cctcgaccga gggagaagtg gccgccgaaa acgcgctggg
      181 ccatcatgtc agcatgaatt atgatgtcgt tcccaatccc atctatacct tccctgagat
      241 tgcttttgta ggactgacgg aagcgcaggc cagagagcaa gatgctactg tccgggtgga
      301 gcggttcccc tgggcggcaa atggcaaggc cgttggcagc gctaatagcg agggcttcac
      361 caaagtcatc ctgggcaagc acaacgagat tctcggtgcg catatcatcg ggccagatgc
      421 gaccaacctg attacggaat attcacttgc gatgcgtggt gaactcacag gtgatgagct
      481 tattgacacg atccacccgc acccaaccct cagcgagggg ttgcgcgagg cggtgctggc
      541 agcagagggt cgtccgatcc acattccacc caggcaagcg gcacgggcaa gatagcagag
      601 aagccctatc ccgctgagag aaaaggaacc tttttctagg aggtgctgat atgatgaagg
      661 acgtaccaga catagagacg aacgctgttg atgtaggcca agttacacca aagactgttg
      721 aacctgctcg tcctgcggtt ccgacggtcc agccccgggt tgtgatcgtt ggggcaggct
      781 ttggcggatt acgggcggca actgccttgc gcgatgtacc tgtccacgtg acggtgatcg
      841 accgcaataa ccatcatctg tttcaaccct tgctctacca ggtagcgact gcctcgctgt
      901 ccccggcaga tatttgtgct cctattcgcc atgtcctgaa aaaacaaaaa aatacggaag
      961 tcttgatggg tgaggtcacc ggcgtggatg tgcaggagca gcgagtattc atgggcgatc
     1021 aagcggttcc ctatgattac ctgattctgg cgacgggcgc acgtgacagc tattttggtc
     1081 acaacgaatg ggcgaaattc gctcctggac tcaaatcgat caggcaggcg acggagattc
     1141 gcagccgaat tctgtgggcc tttgagcagg cggagaagga gactgatgag gagaagcggc
     1201 aggcgttctt aacctttgtg ctggttggtg ctggcccaac cggagtcgag atggccggtt
     1261 ccattgccgg actggcgcat aaaacgctga cagatgaatt tcagcatatc aacccggcag
     1321 acgctcgcat tattctggtt gaagccgtgc caagaatctt gcttgccttc cctgaaaggt
     1381 tggcaaacac agcgaagaat gcgctcaacc agctgggagt cgaagtgcga actaattcac
     1441 ccgtcgagga tgtcaacgat gaaggggtcg tgattggagg caaactcctg cccgcgaaaa
     1501 cggtgatctg gaccgccggg gtggcggcat ctccggcggg gacgtggctg ggagccgaaa
     1561 cggatcgggg ggggcgtgtc aaggtatcca gcgatgtgag tgttcctggt caccctacta
     1621 tctttgtgct tggcgatacg gcgcaattca tgcaggatgg taagccgctt ccaggagtgg
     1681 cacaggtagc gatgcagcag ggaagctatg tggcccgcgt gattgccgat cgggtggcgg
     1741 gaaaagagca caacgcggca tttcgttact tcgataaagg gaatatggct accatcggtc
     1801 gtttccacgg catcgtctca attggcaagc tacaatttgc cggtatactc gcctggttcg
     1861 cgtggctggt cctgcacctc atgttcctga tcggcttccg caatcgcctt gtcgtcatgt
     1921 ttctctgggt ctggtactat acaacctttc aacgcggtgc ccggctcatc acgtctgagg
     1981 acacgtcacg ttccgtgtag cgataggttc gttttccccc cactatgcag gggaggtcag
     2041 cagcatggcc gtggctagag attgggaaag gagttatctt ctatggcaga gagatcaatg
     2101 agcgaacaga ctcccttcga gacctggagt aagttaattg agaacaccat gaaggacaat
     2161 ccggctctgc aaaggaactc agggcaggca gtggaaagtg gaaaagaccc ctggatcacc
     2221 ttgattgaca aactttggca ggccaacccg tccagcaaac ttctccccat cgatcccgct
     2281 gagattaccc atgcctttca gcagatctgg accgatgcgc tgcgtcaccc tgaccggacc
     2341 tgggccaact acgctgactt cgtccagagc tacacacaaa tgatgacggg ggcagccctc
     2401 aaattctggg gacaaggcaa ggatgtcgag ccgaccgtct ctcctgaaaa aggcgacaaa
     2461 cgcttcagca ccccagactg gcagcagaat cccgccttcg atgccctcaa acaaagctac
     2521 ctgctggctt ccacgacgct gctcaagtcc gctgctgagg tgcaggggct ggatgagcac
     2581 cagcagcgca agctca
//
LOCUS       DASZAK010000807         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_339649, whole genome shotgun sequence.
ACCESSION   DASZAK010000807 DASZAK010000000
VERSION     DASZAK010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339649"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..707
                     /locus_tag="VGT44_14270"
     CDS             60..707
                     /locus_tag="VGT44_14270"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEV2582017.1"
                     /translation="MSFDLDSDDQHFLEAMHLHRRHLSEEQMRRYEDYMAEIFTAFGL
                     DLDTPATRETPQRFLRAMFDSTEGYEGDPKLLKVFETECRGGPDCRLSQVVEGPIHFF
                     ALCEHHALPFHGHAYVGYIAHEHIIGISKLTRLVRLFAKRFTVQERIGQQIADTLEAM
                     LEPHGVAVYLEAHHLCTEMRGVRAIAPLTRTTFWRGAYDENAALRAEFFTVCGVH"
     gene            707..1573
                     /locus_tag="VGT44_14275"
     CDS             707..1573
                     /locus_tag="VGT44_14275"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV2582018.1"
                     /translation="MNILTPLETLYAREKDTESKDLPLPPDLLTLYGRLQFPPHPHRP
                     YIIGNFVTTLDGVVSLSIPGHEGGGDISGFNQHDHLVMGLLRAIADAVIVGAGTLRAV
                     PRHRWTAAYIAPTYASVYQELRTRLGKPEPPLNVIVTARGAVSLDLPVFQSGEVPVLL
                     VTTTQGEERIRTQHIPAAVQIAGVQRAGSLSPQAIVQAVCGVRPCQVILVEGGPQLLG
                     DFFAEQLLDELFLTLAPQIAGRDTSTERPGLVTGQRFAPEHPLWGTLVSVKRAGSHLF
                     LRYTFATKETTT"
     gene            1570..>2295
                     /locus_tag="VGT44_14280"
     CDS             1570..>2295
                     /locus_tag="VGT44_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEV2582019.1"
                     /translation="MTNPLLELKALGQSVWLDDIDRGQLRSGAFGRLIEPDGLTGATG
                     NPTIFEHSITHDTTYDEQMQQLLREGKAPKEIYEALAMTDERMVADLLRPTYDRTDGQ
                     DGFVSIEVSPYLAQNKEGTLSEVRRFWQTIDRPNLMVKIPSTPAGIPAIRQAIASGIN
                     INITLIFSLENYRQVAEAYLDALEERRASGKDISHIASVASFFVSRVDVLVDQLLADR
                     MKATGDHAEQQKLTGLRGKAAIAN"
BASE COUNT          476 a          713 c          671 g          435 t
ORIGIN      
        1 cgattcctga agaacagagg caggaaacaa tgatacacta tgaatgaagg agagacacga
       61 tgtcttttga tctggattct gatgaccagc actttctcga agccatgcac ctgcaccgtc
      121 gccacctttc agaggagcaa atgcgccgct atgaagacta catggcggag atcttcaccg
      181 ccttcggctt agacctggat accccggcca cgcgcgagac gccccagcgc tttctgcgag
      241 ccatgttcga ttcgaccgag ggctacgaag gcgatcccaa gctgctcaag gtgttcgaga
      301 ccgagtgccg cggtggaccg gattgccgtc taagccaggt ggtggaaggc cccatccact
      361 tttttgcctt gtgcgaacat catgccctgc cgtttcatgg gcacgcgtac gtgggctaca
      421 tcgcccacga gcatatcatc ggcatttcga aactgacgcg gctggtgcgg ctgtttgcca
      481 agcgctttac ggtgcaagag cgcatcggcc agcagatcgc cgatacgctg gaggcgatgc
      541 tcgagccgca cggggtggcc gtctatctcg aagcccacca cctctgtacc gagatgcgcg
      601 gcgtgcgagc gattgcaccg ctgacgcgca ccaccttctg gcgcggggcc tatgacgaga
      661 atgcagccct acgggccgag ttctttacgg tctgtggggt acactgatga acatcttgac
      721 gccactggaa acgctctatg ccagagagaa agacacagag agcaaggacc tgccactgcc
      781 acctgacctc ttgaccctct atggccgtct ccagttccct ccgcatccac atcggccata
      841 catcatcggg aactttgtca ccacactaga tggggtcgtc tcgctgagta tccccggcca
      901 cgagggcggt ggagacatca gtgggttcaa tcagcacgat cacctggtga tggggttgtt
      961 acgagcgatt gccgacgccg tgatcgtggg ggccgggacc ctgcgggccg tcccccggca
     1021 ccgctggacg gctgcctaca ttgcgcccac gtatgcctcc gtgtaccagg agttgcggac
     1081 gcgcctgggg aagccggagc cgccgctcaa tgtcatcgtg accgctcgcg gtgcggtcag
     1141 cctcgacctg ccggtctttc agtccggtga ggttcccgtg ttgctcgtga cgaccacgca
     1201 aggcgaagag cgcatccgca cacagcatat cccagcagcg gtgcagatcg caggcgtgca
     1261 acgtgctggc tcgctcagtc cccaggcaat tgtccaggcg gtgtgtggag tgcgcccgtg
     1321 ccaggtcatc ctggtcgagg gtgggccaca gttgctaggg gacttctttg ccgagcaact
     1381 cctggatgaa ctgttcttga cgctcgctcc gcagatcgcc ggtcgcgaca cctccactga
     1441 gcgaccagga ctcgtcactg gtcagcgttt cgcgccggag cacccgcttt ggggaaccct
     1501 cgtgagcgtc aaacgcgccg ggagccacct atttctgcgc tatacattcg caacgaagga
     1561 gacaacaaca tgaccaatcc actactggaa ctgaaagcat tggggcaaag tgtctggctc
     1621 gacgacatcg accgggggca actgcgctct ggcgcatttg gacgcctgat cgagccagat
     1681 gggctcaccg gcgcgaccgg caatcccacc atttttgaac actcgattac gcacgatacc
     1741 acctacgatg agcagatgca gcagttgctt cgtgagggca aggctcccaa ggagatctac
     1801 gaagcgctgg caatgaccga tgaacgcatg gtggctgacc tgctgcgccc cacctatgat
     1861 cgcacggacg gacaggatgg cttcgtcagc atcgaagtct cgccctacct ggcacagaac
     1921 aaagaaggga cgctctcgga ggtgcgccgc ttctggcaga ccattgatcg cccgaacctg
     1981 atggtcaaga ttccctcgac cccggcaggc attcctgcta ttcgccaggc gatagcctcg
     2041 ggcatcaaca tcaacatcac cctcatcttc tcccttgaga actaccggca ggtggccgag
     2101 gcctatctcg acgccctgga agagcgcagg gcctcaggca aagacatcag ccacatcgcc
     2161 tcggtagcga gctttttcgt cagccgcgtg gatgtactgg ttgaccaact gctcgcggac
     2221 aggatgaaag ccaccggtga tcatgccgag cagcagaagc tcacggggct ccggggcaaa
     2281 gcagccatcg ccaat
//
LOCUS       DASZAK010000808         1809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_660260, whole genome shotgun sequence.
ACCESSION   DASZAK010000808 DASZAK010000000
VERSION     DASZAK010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660260"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VGT44_14285"
     CDS             <1..1201
                     /locus_tag="VGT44_14285"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEV2582020.1"
                     /translation="INIVHRVFAVFVGLVTLWTVIAALRRWHVARGQAIVGLVGGVLF
                     VCQAIVGGLIVLLNDPAFVTGLHLALATAVWGTLVLLAALASNQLRAAPSQQEIERME
                     QAEAGKELSVLRQTVASYVDLMKPHVTVLLLGVTAAAMAIAYQGMPSLALVVPTLLGG
                     AMAAGSANCINCYIDRDIDQIMGRTQRRSLPAGRVEPRQALDFGILLGIGSFVILATF
                     VNLLSAALACSGILFYVFVYTLGLKRTTPQNIVIGGAAGAVPVLVGWAAITNSLSLSA
                     VALFAIIFFWTPPHFWALALLIQKDYEKAGIPMLPVVKGEAETRRQILLYSVFLLALT
                     LVLFVLGIMGYLYLVGAAFLGGGLVYLAIRLWHDQSRKWARTLFWYSNMYLAAIFAVM
                     VLDRVIH"
     gene            1255..>1809
                     /locus_tag="VGT44_14290"
     CDS             1255..>1809
                     /locus_tag="VGT44_14290"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEV2582021.1"
                     /translation="MLQTVQKAAEVLDLFSVEHPEWGVSELSKKLSFPKSSTSELVST
                     LVEQGLLRRTNSGRYKLGWRIVALSQILLSTTDFRTEARLIMESLIARFGETVHLAAL
                     ESGQVIYIDKLQGTRAVQVSVTGVGHRLPAHCSGVGKAILADYPWEDVIQILNTRGMP
                     PLTPNTITSPETFKQELEQVAAQGY"
BASE COUNT          318 a          526 c          557 g          408 t
ORIGIN      
        1 catcaatatc gtgcatcgcg tcttcgccgt ctttgtcggc cttgtcacgc tctggacggt
       61 gattgcggcc ctgcgccgct ggcatgtcgc gcggggacag gcaatcgtcg ggttggtggg
      121 cggcgtactc tttgtgtgcc aggcgattgt cggcggcttg attgtgctgc tgaacgaccc
      181 cgcctttgta actggcctgc acctggctct ggcgacagcc gtctggggaa cgctggtact
      241 cctggccgcg ctcgcgtcca atcaactgcg cgccgctccg tcgcagcagg aaattgagcg
      301 gatggagcag gccgaggcgg gcaaagaact cagcgtgttg cggcaaaccg tcgccagtta
      361 tgttgatctg atgaagccgc atgtgacggt gctgcttctc ggagtaacgg ctgcggcgat
      421 ggcgattgcc taccagggca tgccttccct ggcgctagtc gtcccgacgc tgctgggcgg
      481 cgcgatggcg gcgggcagcg cgaattgcat caactgctat atcgaccgcg acatcgacca
      541 gatcatggga cgcacacagc gtcgttcgct gcctgccggg cgcgtggaac cgcgacaggc
      601 gctcgatttt ggcatcctcc tgggcatcgg ctcgttcgtc attctcgcaa ccttcgtcaa
      661 tctgctcagc gcggctctgg cctgttcggg catactcttc tacgtcttcg tctatacgct
      721 tggcctcaag cgcaccacgc cgcagaacat cgtgattggc ggcgcggcag gcgcagtgcc
      781 ggtgctggtc ggttgggcgg ccatcacgaa tagcctgagt ttatcggctg tcgcgctctt
      841 cgccatcatc ttcttctgga cgcccccgca tttctgggcg ctcgcattgc tgattcagaa
      901 ggactacgaa aaggcgggca tccccatgct gccggtggtc aagggcgagg cggagacacg
      961 gcggcagatt ctgctttact cggtattctt gctggctctc accctggtgc ttttcgtatt
     1021 gggcatcatg ggctacctct acctggtcgg agccgcgttc ctgggcggcg ggctggtcta
     1081 cctcgcgatt cgcctgtggc acgatcaatc caggaagtgg gcgcgcacgc ttttctggta
     1141 ttcgaatatg tacctggcgg cgatcttcgc cgtcatggtg ctggatcggg ttattcatta
     1201 atggcttgta cataagtcgc cgggagccgc accctggctg aaaggagtgg agatatgttg
     1261 cagacggtac agaaagccgc cgaagtgctc gatctgttct ccgtcgaaca cccagagtgg
     1321 ggggtcagcg agctatccaa aaagctgtca ttccccaaat ccagcacgtc tgaactcgtc
     1381 tccactttgg tggaacaggg tttgcttcgt cgaaccaata gcggcagata caagttgggc
     1441 tggcgcatcg tggcactcag tcagattttg ctttccacta ccgatttccg caccgaggcg
     1501 cgcctcatca tggagtcgtt gattgcccgc tttggggaaa ccgtgcatct tgccgcattg
     1561 gagagcggcc aggtgatcta tatcgataag ctgcaaggca cgcgcgccgt tcaggtatct
     1621 gtcaccggcg tggggcatcg attaccggcg cattgtagtg gagtcggcaa ggctatcctg
     1681 gctgactacc catgggagga cgtaatacaa atcttgaata cgaggggtat gccgccgctc
     1741 acgcccaaca ccatcacgtc gccagaaacc ttcaagcagg aactcgaaca ggttgccgcg
     1801 caggggtac
//
LOCUS       DASZAK010000809         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_20463, whole genome shotgun sequence.
ACCESSION   DASZAK010000809 DASZAK010000000
VERSION     DASZAK010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20463"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VGT44_14295"
     CDS             complement(<1..256)
                     /locus_tag="VGT44_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582022.1"
                     /translation="MIVEAALQENDEAIRQALSANCLCPIIWLMRDLADQTNRSASIG
                     YFEQVAIRITKVKRHKRLCGASPLHRTQKDRNMTGVKMRDN"
     gene            complement(256..>1538)
                     /locus_tag="VGT44_14300"
     CDS             complement(256..>1538)
                     /locus_tag="VGT44_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV2582023.1"
                     /translation="GEFGKCGVAISSLADMEILYKDLPLDKITTSMTINSPAPVIWAM
                     YIVNAEKNGFPRAKLGGTLQNDILKEYSAQKEFLFPPEPSMRLVTDTIEFGTRQMPRW
                     NTISISGYHIREAGATAVQELAFTLADGLAYVDAALARGLDIDEFAPRLSFFFDVHND
                     FFEEIAKFRAGRRLWARLMRERYRAKDPRSWLLRCHAQTAGVSLTAQQPDNNIVRTTI
                     QALASVLGGTNSLHTNSLDEALALPTERAALIALRTQQIIASESGVVNTVDPLGGSYF
                     VESLTDQTEQAAMDYICRIDALGGVLACIQNGFFQREIAESAYRYQQEVDTHKRTIVG
                     VNDYIMDEEVKVPTLYIDRVGEMAHLERLERVRKERDQDAVKCALVALRRVAEGTENT
                     MPAIIEAVKTYATLGEIMDVFRSVFGDYMEPAVF"
BASE COUNT          300 a          441 c          455 g          342 t
ORIGIN      
        1 tgttatcgcg catcttcact cctgtcatgt ttctgtcctt ctgggtccgg tggagcggac
       61 tggcgccaca aagtctcttg tgtctcttta ctttcgtgat cctgatggca acctgctcga
      121 agtagccaat gctcgctgat cggtttgttt gatcagcgag atcgcgcatg agccagatta
      181 tggggcagag gcagttcgct gaaagcgctt ggcgaatagc ctcgtcattt tcttgcaaag
      241 cagcctcgac gatcattaga acactgctgg ctccatatag tcgccgaaga ctgaacggaa
      301 gacatccatg atctcgccca gcgtggcgta ggtcttgacc gcctcgataa tcgcgggcat
      361 ggtgttctcg gtaccctcgg cgacgcggcg cagcgcgacc agtgcgcact tcaccgcgtc
      421 ctggtcgcgc tctttgcgca cgcgctccag gcgttccagg tgggccatct cgcctacacg
      481 atcaatgtag agggtcggaa ccttgacctc ttcgtccatg atgtagtcat tgacgcctac
      541 aattgtgcgc ttgtgcgtgt caacctcctg ctgataacga taggccgact cggcgatctc
      601 gcgctggaag aagccgttct ggatgcaggc cagcacaccg cccagcgcgt caatgcggca
      661 aatatagtcc attgctgcct gctcggtctg atcggtcaag gactcgacaa agtaggagcc
      721 gccgagagga tcgacggtgt tgaccacccc gctctcggag gcgataattt gttgggtgcg
      781 cagggcgatt aaagcggccc tctcggttgg cagcgccagg gcctcgtcga gcgaattggt
      841 gtgcagcgag ttggtgccgc ccagcaccga ggctaaagcc tggatggtcg tgcggacgat
      901 attgttatcc ggctgttgcg cggtcagcga gacaccagcg gtctgcgcgt ggcagcgcag
      961 cagccaggag cgcggatctt tcgccctgta gcgttcgcgc atcaggcgtg cccacaggcg
     1021 gcgtccagcg cggaacttgg cgatttcttc aaagaaatcg ttatgcacat cgaagaagaa
     1081 cgagagacgc ggcgcgaact catcaatgtc gagcccgcgc gccagtgccg catcaacata
     1141 ggccagacca tcggcgagcg tgaaggccag ctcttgcacc gccgtcgcgc ccgcctcgcg
     1201 gatatgatag ccgctgatac tgatcgtgtt ccagcgcggc atctggcgcg tgccaaactc
     1261 aatcgtatcc gtcaccaggc gcatcgaggg ttctggcggg aagaggaact ctttttgcgc
     1321 actatactct ttgagaatgt cgttttgcaa tgtgccgccc aattttgcgc gtgggaagcc
     1381 gttcttttcg gcgttgacga tatacatggc ccagatgacc ggagccgggc tattaatcgt
     1441 cattgaggtc gtgattttat ccaggggcag atctttatag aggatctcca tatcggccag
     1501 cgacgagatg gccacgccac acttgccgaa ctcgccag
//
LOCUS       DASZAK010000810         4782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_552438, whole genome shotgun sequence.
ACCESSION   DASZAK010000810 DASZAK010000000
VERSION     DASZAK010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552438"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            215..937
                     /locus_tag="VGT44_14305"
     CDS             215..937
                     /locus_tag="VGT44_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582024.1"
                     /translation="MIKITLSYRTAVLCVALVALAIAGTVFAQTSGASSGSDAGRLVR
                     SQSFQALTLSQMVQAHQLASTVQVLSSQAPSVIIQFPLFPSGVKAKFPQATGVVTIAE
                     GNPAVSLFDSVTVDVSHMPPDITFTIFFIELANKPFGHVEYVADLHTRSDGSGESSFQ
                     AITLVAFAADNRTPTVTSQDQSGAASGIQLEHLGMWFSSLQDAQKTLSNNALTGTLFD
                     GGNPPLHAGPQAMTDGQTAPVF"
     gene            978..1736
                     /locus_tag="VGT44_14310"
     CDS             978..1736
                     /locus_tag="VGT44_14310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582025.1"
                     /translation="MLLLHPLRMLRRVALSSALLAALLIVVSSPQAGAAIAATRAPAS
                     PGIQHAAQVSAELQNPEAAQLRQLVREGRLASTVHVLNSLAPTTIVQFPLIPSGIKAA
                     FPQATGLVTIIRGNRDNAIADTVIVDVQNMPPNITFTIFYIQFAGKPFGNVEYVADLT
                     TRGDGSGETLFQNIAFVAFAMDARNPGTSIDGQEGLTSGTNLEHMGMWFSSLQDAQSV
                     LNDDSLTGTIFDGGNPPQHAGPQAMTDGQSAPVF"
     gene            1754..2482
                     /locus_tag="VGT44_14315"
     CDS             1754..2482
                     /locus_tag="VGT44_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582026.1"
                     /translation="MIKRTSRIKWWFIALVLVALLVAACDTSPTTTPGSTGTTTTTGG
                     GAPFPTTPLTLRTPSFGSQEASTVQVLSSADPTQIIQFPLFPVSIKALFPKATALVTV
                     IEGNPAISVFDTVIVDVQNMPPNQKFTVFLVELANKPFGHGEYVGDVITRGDGSAESI
                     FHLITFVAFAADNRTPNVASADQSGQASGIQLEHVGMWFDGVIAARQVLNNPNIPGTP
                     FDGGGGALHAGPQAMTDGQTAPVI"
     gene            2654..3094
                     /locus_tag="VGT44_14320"
     CDS             2654..3094
                     /locus_tag="VGT44_14320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582027.1"
                     /translation="MPKYQRFSPPERVLSLLAGLVNLVIGLGFFFLPEFHPALWPTSI
                     PPILDRFIGAIILGNAAGAIWLCREREWARVRPLAIVAVVYGTLVAIALLYHLLALNA
                     APIFWVYFLTDVPFLLVYYALFIYHDLMPRLGKQNARSLQHDQG"
     gene            3128..>4782
                     /locus_tag="VGT44_14325"
     CDS             3128..>4782
                     /locus_tag="VGT44_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2582028.1"
                     /translation="MQAPFADPLPGIALGTQRLPLVGRAWEMQMIASVLETVAFDRPA
                     GARALLISGDVGIGKSRLLAEMRDRAQERSFQVLDGRVYESGMMLPYLPFVEALRPVI
                     RSISLDTLRGYTGLRAKHPQVLNPELVGEPETTLVEPSLSLSGVPMVTALARLFPELP
                     GLLRVTPLSEVLSQDQEKFRLFDAIATLLERMAEDQPVLLGIDNLQWADSASLELTMY
                     LTARLRQSRVALIGVTRPPSSTRAKMADNSGDAVSGAMAIRMLGELVRQGLLLVLPLG
                     PLAPDETERYLHALLPGEIPANLVNALHSRVEGNPFFLEELVRMLTLQGQMFLQDGVW
                     RTSRPIGSDLPQSIHQAVEQRLQGLSLPCQERLRAASLFGRRFPLAALAAALRESEAK
                     AQLFIDEAVQAGVLARTSASEPVWWDGQVDQAELPSMSYTFYTPLASNASDEYIFCQG
                     IVQEVLSSQEPAYRARELHGEIGAALETYYGTQAPAAELAYHYTLGSVQRPALRWSVL
                     AGEEALRKQAYREAIGHFRVALQLIDAQSDAHFDDTVPSPVQLS"
BASE COUNT          980 a         1430 c         1344 g         1028 t
ORIGIN      
        1 ccagatcttc gccagccgag accggggcga aagctggaac ctgatcgccg acttcctgcc
       61 atcggtctac tcggttcagg cgttcgagat cgaataaaac ctctcatgaa aatgggtaat
      121 cttgcgcatg tttctcttgg agaaaccctg tacgcttaca gacgaggaat agctctggtt
      181 ggagtatcag ggctgcaaat ccaatggagc gacaatgatc aagattaccc tttcctatcg
      241 taccgccgtg ctatgtgtgg cgctggtggc gctggccatc gccgggacgg tcttcgcgca
      301 gacgagtgga gcaagttcgg gcagtgatgc cggtcggttg gtgcgcagtc aaagtttcca
      361 ggcgctgacg ctgagccaga tggtacaggc gcatcaactt gcctcgaccg tgcaggtgct
      421 gtcctcgcaa gcgcccagtg tcatcatcca gttcccgctc ttccccagcg gggtcaaggc
      481 caaatttcca caggcaacgg gcgtcgtgac catcgccgag ggcaaccctg ccgtcagcct
      541 gttcgactcg gtgacggtcg atgtgtccca catgccgccc gacatcactt tcaccatctt
      601 cttcatcgag ctggccaaca agcccttcgg acatgtggag tacgtggccg acctgcacac
      661 gcgcagcgac ggctccggcg agtcctcgtt ccaggccatc accctggtcg ccttcgccgc
      721 cgataaccgc acgccgaccg tcacctcaca agaccagagc ggcgcagctt cgggaattca
      781 actggaacat ctgggcatgt ggttctcctc gcttcaggac gcgcagaaga cgctgagcaa
      841 taacgctctg accggcacgc tcttcgatgg aggcaatcct cccctgcacg ccgggccgca
      901 ggccatgacc gacggccaga ccgcgccggt gttttaatgc gcctgatcga caagcatctc
      961 accagaagga gaaaccaatg cttttactgc accctttacg gatgctgcgc cgagtcgcgc
     1021 taagtagcgc gctgctggct gcgctgctca tagtagtaag cagcccgcaa gcaggcgcgg
     1081 ccatagctgc cacgcgcgca cccgcctctc caggcatcca gcacgccgcg caggtcagcg
     1141 ctgaactaca gaatcccgaa gccgcgcaat tgcgccagtt ggtgcgtgag ggccgcctag
     1201 cctcgacggt acacgtgctg aattcgctcg cgccgacgac tatcgtgcaa tttccgctta
     1261 ttccaagtgg gatcaaggcc gcgtttcccc aggcgacggg gctggtgacg atcattcgag
     1321 ggaaccggga taatgcgatt gccgataccg tgattgtcga tgtgcagaat atgccaccca
     1381 atattacctt caccattttc tatatccagt ttgctggtaa accatttgga aatgtggagt
     1441 atgtggccga tctgaccaca cgcggtgatg gcagcggcga gaccctgttc cagaatatcg
     1501 cgtttgtcgc ctttgcgatg gatgcgcgca atcccggcac ctcgattgat ggccaggagg
     1561 gtctaacatc gggaacgaat cttgaacata tggggatgtg gttctcctcg ttgcaggatg
     1621 cgcagagcgt cctgaacgat gatagcctga ccggcacgat cttcgacgga ggcaatcccc
     1681 ctcaacatgc cggaccgcag gccatgaccg atgggcaaag cgccccggta ttctaaagag
     1741 gagccagaat agcatgataa aacgtacctc gcgcatcaaa tggtggttca tcgcgctcgt
     1801 gcttgtcgcc ttgctcgttg cggcatgtga tacttccccc accaccacgc cgggcagtac
     1861 cggcacgacg acgacgacag gcggtggagc gccctttcca accacgccgc tgaccttacg
     1921 tacaccttca ttcgggagcc aggaggcgtc aacggtccag gtcctctcct cagctgatcc
     1981 gacgcagatc atccagtttc ccctgtttcc ggtgagcatc aaagcgctgt tcccaaaagc
     2041 gacggccctg gtcacggtta tcgagggcaa cccggcgatc agcgtgtttg ataccgtcat
     2101 cgtcgatgtg cagaacatgc cgcccaacca gaaattcacc gtgtttctcg ttgaactcgc
     2161 caataaaccc ttcggacatg gcgaatatgt cggcgatgtc atcacgcgcg gcgacggcag
     2221 cgccgagtcg atcttccacc tgattacctt cgtcgccttc gctgccgata atcgcacgcc
     2281 gaacgtcgcc tcggctgacc agagcggcca ggcgtcaggc atccagctgg agcatgtcgg
     2341 catgtggttc gacggcgtga ttgccgcccg ccaggtgttg aataatccca atatccccgg
     2401 tacgcccttc gatggcggcg gtggcgccct acacgccggg ccgcaggcca tgaccgacgg
     2461 gcaaaccgcg ccggtgatct agccaacaag tctgaggcat cctcgtatcc agcgacaggt
     2521 gcgcacggca ttcctgctca tgaaaaattc aagggcagga atgctttttg tcgtggagca
     2581 agaccctact gtttcattct caagtggtgt ataatcctag cgaactgtta ttgagaaagg
     2641 ggcagccgct gctatgccaa agtaccaacg cttcagcccg ccggaacgag tgctttcatt
     2701 gctcgccggg ttagtcaacc tggtcatcgg cctgggtttc ttctttttgc cggagtttca
     2761 cccggcgctg tggccaacga gcatccctcc aattttggat cgcttcattg gagcaattat
     2821 cctcggtaat gcagctggcg ctatatggct ttgccgggag cgcgaatggg ccagggtgcg
     2881 gcctctggca atcgtggcgg tcgtctatgg cacactggta gcgatagcac tgttgtacca
     2941 cctgctcgcg ttaaatgccg ccccgatttt ctgggtgtat ttcctgaccg atgtcccttt
     3001 tctgctcgtc tactatgccc tgttcatcta tcatgacttg atgccgcgcc tcggaaaaca
     3061 gaacgcgcgc tcacttcagc acgatcaagg ttaaacagag catcgccttc gaaagggagc
     3121 cggaatcatg caagcgcctt tcgccgatcc attgccaggt atcgcgctgg gaacacagcg
     3181 cttaccactg gtcggtcgag catgggagat gcagatgatc gcctcggttt tggagacggt
     3241 cgccttcgat cgaccggctg gcgcgcgcgc cttactcatc agcggcgatg tgggcattgg
     3301 aaaatcacgc ctgctggccg aaatgcgtga tagagcgcag gaacgatcat ttcaggtgct
     3361 ggatggccgt gtctatgaat cgggtatgat gctcccgtat ctgcctttcg tcgaagcgct
     3421 gagaccggtg atacgctcga tatcgcttga tacattgcgc ggatataccg gacttcgcgc
     3481 aaaacatcct caagtactca accctgaact ggttggagaa ccagagacta cgctagtaga
     3541 accatcgctc tcattgagtg gcgtaccgat ggtaacagcg ctcgcgcgcc tcttccccga
     3601 attgcccgga ttgttgcgcg tcactcccct atcggaggtg ctatcgcaag accaggagaa
     3661 gttccgcctc tttgatgcta ttgcaacgtt gctggaacgc atggcagagg atcaaccggt
     3721 cttgctgggc attgataacc tgcaatgggc ggatagcgcc agcctggaac tgacaatgta
     3781 cctgaccgcg cgcctgcgcc agagcagggt tgccctgatc ggtgtgactc gtcctcccag
     3841 ctcgacaaga gcaaaaatgg cggacaattc cggtgatgct gtgtctggag ctatggctat
     3901 aagaatgttg ggcgagctgg tgcgccaggg attgctgctg gttctgccat tgggtccgct
     3961 cgctcccgac gaaaccgagc gctacctcca tgccctgctg cccggcgaaa tcccggccaa
     4021 ccttgtcaac gccttgcatt cacgagtcga aggcaatccc tttttccttg aagagctggt
     4081 gcgcatgtta acgctacaag gccagatgtt cttgcaagac ggcgtgtggc gcacgtcgcg
     4141 accaatcggt tctgatcttc cacagagtat tcaccaggcg gtcgaacagc gtttgcaggg
     4201 gttgagcctg ccatgtcagg agcgtttgcg cgctgcatca ctttttggac gcagattccc
     4261 cctggcggct ctggcagcag ccttacgcga gtccgaggcc aaagcgcaac tattcattga
     4321 cgaggctgtc caggcaggtg tgctggcccg tacatcagcc agcgagcctg tctggtggga
     4381 tggacaggtg gatcaagcag aactaccttc gatgtcctac acgttctata ctccgcttgc
     4441 atccaacgct tccgatgaat atatcttctg ccagggcatt gttcaggaag tattaagcag
     4501 ccaggagcct gcctatcgcg cacgagagct gcacggcgag atcggcgcgg cgttggagac
     4561 gtattatgga acgcaagcgc ccgccgccga actggcgtat cactatacac ttggcagcgt
     4621 gcagcgacct gctttgcgct ggagcgtcct ggccggcgag gaggccctac gcaaacaggc
     4681 ttatcgcgaa gccatcggtc attttcgcgt tgccctccaa ttaatcgatg cgcaaagtga
     4741 cgctcacttt gacgacacag tgccatcgcc tgtccaactc tc
//
LOCUS       DASZAK010000811         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_20588, whole genome shotgun sequence.
ACCESSION   DASZAK010000811 DASZAK010000000
VERSION     DASZAK010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20588"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VGT44_14330"
     CDS             <1..193
                     /locus_tag="VGT44_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582029.1"
                     /translation="QGQDFKLYINGIFVGETQDSTLTKGEIAFASGTDQSETSSEASY
                     SNLKVFRISANSSSTPQGS"
     gene            339..1562
                     /locus_tag="VGT44_14335"
     CDS             339..1562
                     /locus_tag="VGT44_14335"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV2582030.1"
                     /translation="MAEDGISSLDLSLYGDIVVLDLTSNVRGMHCAKLFADYGADVIL
                     VEPLEGSFARQIGPFAYGRQDRETSLHFTHHNRNKRSLRLNLQSERGQQILRDLASGA
                     DVLIEDWPVGALDALGLGYEQLHVLNPRLVYTSITGFGQSGPYAHFKTSNIVEDAMGG
                     LMNITGPANKPPTMTGAQQAEHVTAVHAAFATAAALLYRDLHGIGQRVDVSAQECTGS
                     VLENAVEQYTYSGVVRKRSGSRHGTAWPCTVFPCNDGYIGMCCTQAKEVRAAVLLAED
                     FELAEDPVLENTFERRARADELEPRLIAGYMKYGKAQLFHKGQSLGCPIGMTSDARDL
                     ATDPHLNEVGFFAEFHHPVIGAYKDIAAPVYMSETPGVMRSAAPLLGQHNDEILRELG
                     LSDAELAALRADGVM"
     gene            1581..>2019
                     /locus_tag="VGT44_14340"
     CDS             1581..>2019
                     /locus_tag="VGT44_14340"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV2582031.1"
                     /translation="MLKDALAGFRCVDFSWVFAGPYCGQLLGDLGMEMIKIECRKRMD
                     VVRMYPPFAFEDRRLNSSGYWNTHNRSKYSVNLDMKSEQAREIVTRLVAMSDVVLENY
                     SARGMKAIGFDYERLREINPRIVYCSMAGFGHRGPYKNYVAYGP"
BASE COUNT          435 a          570 c          597 g          417 t
ORIGIN      
        1 tcaaggccaa gatttcaaat tgtatatcaa tgggattttc gtaggtgaaa cacaggatag
       61 cacccttact aagggagaga ttgcttttgc ttcgggaacc gatcagtcag aaacgagtag
      121 cgaggccagt tattctaatc ttaaagtgtt tcgtataagc gctaactctt cttctacccc
      181 gcagggatcc taactaatcg aaggtttaga agagcgccct ccaaagtatg acgaccgcta
      241 atggtccact ctcaacggtt gtgatatact aatgccatat ttcaggcgtc ttctctccat
      301 tctaccctga tcagtaattg gcaggaagga actgtactat ggccgaagat ggtatctcct
      361 ctctcgatct gtcgctctac ggcgacattg tggtactcga tctgacgagc aacgtgcgtg
      421 ggatgcattg cgccaaacta tttgctgact atggcgcgga cgtgatcctg gtcgagccgc
      481 tcgaaggcag tttcgcgcga caaattggcc cctttgccta tggcaggcag gaccgcgaaa
      541 cgagcctgca cttcacgcat cacaaccgca ataagcgcag tctgagactg aacttgcaat
      601 ccgaacgcgg gcagcaaatc ttgcgcgacc tggcgagcgg ggccgatgtg ttgatcgaag
      661 actggcccgt cggcgcgctg gatgcgctgg ggttgggcta cgaacaactc cacgttttga
      721 atccgcgcct ggtctatacg tccatcaccg gctttggaca gtcgggaccg tatgcgcatt
      781 tcaagaccag caatatcgtc gaagatgcca tgggcggctt aatgaacatc accggcccgg
      841 ccaataagcc gccaaccatg accggcgcgc agcaggccga gcatgtcacc gccgtgcatg
      901 ccgcctttgc cacggctgcc gccctgctct atcgcgacct gcacggcatc ggccagcgcg
      961 tggatgtctc cgcgcaggaa tgcaccggct ctgtgctgga aaacgccgtc gagcagtaca
     1021 cctacagcgg tgtggtgcgc aagcgttccg gcagtcggca tggtactgcc tggccctgca
     1081 ccgtctttcc ttgcaacgat ggctatatcg gcatgtgctg cacccaggcg aaagaggtga
     1141 gagcggcggt gcttctggcg gaagatttcg agctggcgga agaccctgtg ctggagaata
     1201 cgtttgaacg gcgcgcccgc gccgacgaac tggagccgcg cctgatcgca ggctatatga
     1261 aatatggcaa ggcgcagctc ttccacaaag ggcaatcgct gggatgtccc atcggcatga
     1321 ccagcgacgc gcgcgacctc gccaccgacc cacatttgaa cgaggtcggt ttctttgccg
     1381 agttccatca cccggtcatc ggcgcttaca aggacattgc cgcgccggtc tatatgtccg
     1441 agacgccggg agtgatgcgc agcgccgccc cgctgctggg acaacataac gacgagatac
     1501 tgcgcgagtt gggactgagc gatgctgagc tggccgcgct gcgcgcggac ggcgtcatgt
     1561 aaggagagag gagagcaagc atgttgaaag acgcactggc aggttttcgc tgcgttgact
     1621 tttcgtgggt cttcgccggg ccatactgcg ggcaattgct cggcgacctc ggcatggaga
     1681 tgattaagat cgagtgccgc aagcgcatgg acgtggtgcg catgtacccg cccttcgcct
     1741 tcgaggaccg ccgcctcaac tccagcggct actggaatac gcacaatcgc agcaaataca
     1801 gcgtcaacct ggatatgaag agcgagcagg cgcgcgagat tgtgacccgc ctggtggcga
     1861 tgagcgacgt ggtgctggaa aactacagcg cgcgcggcat gaaggctatc ggcttcgact
     1921 atgagcgcct gcgcgagatt aacccgcgca tcgtgtattg ctcgatggcg ggttttgggc
     1981 atcgcggccc ctataagaac tatgtcgcct atggtccga
//
LOCUS       DASZAK010000812         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_126204, whole genome shotgun sequence.
ACCESSION   DASZAK010000812 DASZAK010000000
VERSION     DASZAK010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126204"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="VGT44_14345"
     CDS             complement(<1..628)
                     /locus_tag="VGT44_14345"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV2582032.1"
                     /translation="MVWVVPVLHHINPWLLCLVVAIILLLESCGIPILNSSLLLFAGA
                     VASLGHVNIIVLALAAIIGSTSGACLAYLIGLRGGELALNRLLTRLHVNTRRIEQARG
                     WFANAGGRMILLSRILPYVRPFACFFGGIAQMPFRRFLAAALSGSILWCVAALSVGWM
                     LGRRWRIALYLIQSYTLPTLGVLVLAIAVYILAKVLINRRRSRKRQQAD"
     gene            926..2275
                     /locus_tag="VGT44_14350"
     CDS             926..2275
                     /locus_tag="VGT44_14350"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582033.1"
                     /translation="MSTTASNAGDTAIKDRLKPTRSQAGFAFSVLLVINILNYADRYV
                     LPAILPKIADPKQGLGLTPFQQGLLGSSFLIVYALATLPLGVWADRGVRKNIVALCVG
                     IWSVATTLAGITRSFAQLFSVRAVLGIGEAGYAPASLSLLGDFFPKSQRGRILSYWSA
                     GTLIGAAIGFTLGGLIADTLGWRYAFFVVGVPGLIAAFLSWRMVEPVKGISDMNDVIV
                     ESEGTPVIEHGNIGKGFWKTAGALRGIPTYWVLVGALVFSFFTIGGTSFWLPSYIVDS
                     FRLSVGQAGLASGAVLVASGLIGTVVGGWLADFAQRRRPEGRLLIATLGLLIGAPLVL
                     GALLLHTLALFIAVFVLAAIALNFCTGPLNAVIQDVIAPEMRATALGLSLLLAHLLGD
                     AAAPTVIGAIANGTSLGTALTLTAPTFLFLAGLVCLLGLRTVARDMRRMQQQLLTGK"
BASE COUNT          455 a          670 c          694 g          476 t
ORIGIN      
        1 agtcggcctg ctgccgtttg cgcgatcggc ggcgattgat gagtaccttt gccaggatgt
       61 agacggcgat tgccagaaca agcacaccaa gcgtggggag agtataggat tggatcaggt
      121 acagggcgat cctccacctg cggccaagca tccagccgac agagagggcg gccacgcacc
      181 agaggatcga accgcttagc gcggcagcta agaagcggcg gaagggcatc tgcgcgatac
      241 cgccgaagaa acaggcgaaa ggccggacgt aaggcaggat gcgcgaaaga aggatcatgc
      301 gaccgcctgc gttggcaaac cagcccctgg cctgctctat gcggcgggta ttcacgtgca
      361 gacgggttag aagacggttc aacgccagtt cgccgccgcg caggccaatc agataggcca
      421 gacacgcgcc cgatgtgctg ccgatgatgg cggcaagcgc gagaacgatg atatttacat
      481 gccccaagga cgcaacagct cccgcgaaca gcagcaggct actattcaat atcggaatgc
      541 cgcagctttc cagcagcagg attattgcca ccacaaggca caatagccag gggttgatat
      601 ggtgtaaaac aggcacaacc cagaccaaaa ctttttcttc ctccgcagta gctcgcgctt
      661 ccctgcagcg tcttcagaga gtcaccatct ctgttcgatc ctacccggtc tctttattag
      721 tatcgtctct tccctgtgtt catacgaacg atccttccac acggttacat tgcctataca
      781 atcatgaaga tgtctctcat gcttcaaaca gccagtaggg ggccgcaggt aatgctcttt
      841 cctacttttt agacgcgcca gcgtagatat tttgatgagg tactatgaga tataatgatg
      901 gaaaaagtga aagcataagg aaagcatgtc aacaactgca tcgaacgcgg gcgatacagc
      961 aatcaaagat cgcctgaaac ccacgcgaag ccaggcgggc ttcgctttta gcgtcctgct
     1021 cgttatcaac atcttgaact atgccgaccg gtacgtcttg cctgctatat tgccgaaaat
     1081 cgccgacccg aaacagggcc tgggcctgac cccttttcaa caggggctgc ttgggtcatc
     1141 gttcttgatc gtatatgccc tggcgacgct gccactgggg gtatgggctg atcgaggagt
     1201 gcgtaaaaat atcgtcgcgc tgtgcgtcgg catctggagc gtggcaacca ccctcgcggg
     1261 catcacgcgc agtttcgcgc agttgttcag tgtgcgcgcg gtgctgggca tcggggaagc
     1321 aggctacgct ccggcttcac tttcgctgct cggtgatttc ttcccaaagt cacagcgcgg
     1381 acgcatcctg tcctactggt cggctggcac cttgatcggc gcggccatcg gcttcacgct
     1441 cggcgggctg atcgcagata cgctgggctg gcgctacgcc ttcttcgtcg tgggagtgcc
     1501 agggctgatc gcggccttcc tatcctggcg catggtagaa cccgtcaagg gcatctctga
     1561 tatgaatgac gtgatcgtcg agagcgaggg gacgcccgtc atagaacatg gcaatatcgg
     1621 gaaggggttt tggaaaacag ccggcgcgct gcgcggcatt ccgacctact gggtgctggt
     1681 gggggcgctg gtgttcagct tcttcacgat cggcggcacc tcgttctggc tgccatccta
     1741 tatcgtcgat agcttccggc tcagtgtggg gcaggccgga ctcgcttcgg gcgcggtgct
     1801 ggtcgcaagc gggctgatcg gcacagttgt cggcggctgg ctggccgact tcgcgcagcg
     1861 gcgtcgtcca gagggccgcc tgctgattgc cacgctgggt ctgctgatcg gcgcgcccct
     1921 ggtattggga gcgctgctcc tgcacacgct cgcgctcttt atcgctgtct ttgtcctggc
     1981 cgccatcgcg ctgaattttt gcaccgggcc gctgaacgcc gtgatccagg atgtgattgc
     2041 gcccgaaatg cgggcgaccg cgctgggtct ctcgctgctc ctggcgcacc tgctcggcga
     2101 cgccgcggcg cccaccgtca tcggcgccat cgccaacggc acatcgcttg gaaccgcttt
     2161 gacgctgacc gccccgacct tcctgtttct ggccggcctg gtctgcctgc ttgggctgag
     2221 aacggtggcg cgtgacatgc gcaggatgca gcagcagtta ctgaccggga aatgagcgta
     2281 ctgaacacag aacgg
//
LOCUS       DASZAK010000813         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_589337, whole genome shotgun sequence.
ACCESSION   DASZAK010000813 DASZAK010000000
VERSION     DASZAK010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589337"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VGT44_14355"
     CDS             <1..902
                     /locus_tag="VGT44_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEV2582034.1"
                     /translation="TNLEAATGCTVILCDMPTMGGVDVRGGAPATRETDLLRPLNLVD
                     HVNAVLLTGGSAFGLDAASGVMRYLEERDIGYETGVARVPLVPAAALFDLAVGSTSIR
                     PDAEAGYRACQQASSKAVEQGTVGAGTGATVGKMAGPDSMMKGGLGSASLLLPDGTLV
                     GAIVAVNALGDVIDPQTQQVVAGARNPGGSGFLASSPFGNTTIAVVATSAALTKEQVN
                     KVAQMAHNGLAQAIRPAHTMFDGDTVFALALGSSSQTLQNSQLAAAFVSAIGAAAAIT
                     LARAIVKAVRHATTLHGIPAIHS"
     gene            complement(960..1202)
                     /locus_tag="VGT44_14360"
     CDS             complement(960..1202)
                     /locus_tag="VGT44_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582035.1"
                     /translation="MQARRRAIDFAGNSWDLATGGLKTMTAKRELPPRLERHHDDQPA
                     VRSPAYRSYTFALVILACVIVMLVGGGIVLFVMLQP"
     gene            complement(1567..>2245)
                     /locus_tag="VGT44_14365"
     CDS             complement(1567..>2245)
                     /locus_tag="VGT44_14365"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEV2582036.1"
                     /translation="AMNFAPTQQKRNGCPSHLCYNNSMDYQNIHTVVQVLRERLEPVE
                     RADLLIDALEGQRPTARKAAVLICLFDQNAESRLVFIRRATTLRSHSGEIAFPGGSVD
                     HQDASFAMTALREAQEEIGLDPARVELLGIMPPVFTVVSNFLITPVVAYLPRGPGELR
                     LQPSEVDELLIFPLEGLANPAIYHTEEWTRGGVARTVYFFDYGSNRIWGATARMLNAL
                     LDVLRRS"
BASE COUNT          483 a          629 c          702 g          431 t
ORIGIN      
        1 acaccaacct tgaggccgcc accggctgca ccgtaatcct gtgcgatatg ccgacgatgg
       61 gcggcgtcga tgtgcgcggg ggggcgcccg ccacgcgcga gaccgatctg ctgcgaccgc
      121 tcaacctggt ggatcacgtc aacgctgtgc tgctgacggg tgggagcgcg tttgggctgg
      181 acgcggcaag cggcgtgatg cgctacctgg aggagcgcga tattgggtat gagaccggcg
      241 tggcgcgcgt accgctcgtc ccggcagctg cccttttcga cctggccgtt ggctcgacca
      301 gtatccggcc tgacgcggag gcgggttatc gcgcctgtca gcaggctagc agcaaggccg
      361 tcgagcaggg aacggtcggc gcgggtacgg gcgcgacggt gggcaagatg gcgggtccgg
      421 atagcatgat gaagggcgga cttggctcag ccagtctgtt gctgccggat ggcacactcg
      481 tcggcgcgat cgtggcggtc aacgcactgg gcgacgtgat cgatccgcag acgcaacagg
      541 tcgtcgcggg cgcgcgaaac cctggcggct ccggcttcct ggcgagcagc ccgtttggca
      601 acacaaccat cgcggtcgtc gccaccagcg ccgcattgac gaaggaacag gtcaataagg
      661 tcgcgcaaat ggcgcacaac ggcctggcac aggcgattcg gcctgcgcat actatgtttg
      721 atggagatac agtgttcgcg ctcgcgctgg ggtcatcctc acaaacgctt caaaactcgc
      781 aactcgcggc ggcgttcgtc agcgccatcg gcgcggccgc agccatcaca ctcgcgcgag
      841 ctattgttaa agcggttcgt catgccacca cgttacacgg cataccagcg atccattcgt
      901 agcagcctcc cttgcttgta tcccccgatg cggcagttca ggcagaacat cgcttctcat
      961 cagggctgca acattacgaa taatacaatg cctccgccca ccagcatgac gatgacacag
     1021 gccaggatca ccagtgcaaa ggtgtaactg cgatatgccg gggaccgcac agccggctgg
     1081 tcatcgtggt gtcgttccag gcgtggaggt agttctcgtt tggcagtcat agtcttcagg
     1141 ccaccggtcg caaggtccca ggaattgcca gcaaaatcaa tggcgcggcg tcgcgcctgc
     1201 atctctggag attctgctcg ttcgctcggg gaattgagtg aggtgagtgc aggggtcata
     1261 ggcggcgcgg ggtatggcaa ttgaggcaat cgagtagcgg gtatgttcga gtgaggtgag
     1321 ggtgtaggcg atggttgagg cagaaaattt ggagagcgcc catcaggtgt tggcaatgtc
     1381 acagttttct ccaccaggga ttaaagtcca accgcttcac acgctacaaa aacaatctac
     1441 gagacacgct ctacgtataa gacctacgga ataattatgc cggacgtgaa tggacttaac
     1501 cactcgctct atattgtatc aaacgaacga actggataat taggaacatg acttatgggt
     1561 tgcctattac gaacggcgca acacatcgag caaagcgttg agcattcgcg cggtcgcgcc
     1621 ccagatgcgg ttggagccat aatcgaagaa atagacggtg cgcgccacgc caccgcgtgt
     1681 ccattcctca gtatggtaga tggcagggtt ggccagtccc tccaatggga aaatgagcaa
     1741 ttcgtctacc tcgctcggct gcaagcgcaa ttcgcccggt ccacgcggca aataggccac
     1801 gacgggagtt atcagaaaat tgctgacaac ggtgaagacg ggaggcatga tacccagcaa
     1861 ctcgacgcgc gccggatcaa gaccgatctc ttcctgcgcc tcgcgcagag cggtcatagc
     1921 aaacgaagca tcctggtggt ctacagagcc gcccggaaag gcgatttcac cgctgtgcga
     1981 gcgcaaggtg gtggcgcggc gaatgaagac caggcgcgat tcggcgttct ggtcaaaaag
     2041 gcagatgagg actgccgctt ttcgagcagt cggacgctgc ccctctaatg catcgatgag
     2101 caagtcggcg cgctcgaccg gctccaggcg ttcccgcagc acctgcacaa cagtatgaat
     2161 gttctggtaa tccatgctgt tattgtagca cagatgagat gggcaaccat ttcgtttttg
     2221 ctgtgtaggg gcgaaattca ttgcg
//
LOCUS       DASZAK010000814         7630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_92432, whole genome shotgun sequence.
ACCESSION   DASZAK010000814 DASZAK010000000
VERSION     DASZAK010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7630
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92432"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VGT44_14370"
     CDS             complement(<1..245)
                     /locus_tag="VGT44_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2582037.1"
                     /translation="MSSLAGKVAVVTGGNSGIGLATAREFHANGAKVVISGRDRETLE
                     EVSRELGHNVLVVPTDVTKLNEIDQLMARAYETFGKL"
     gene            519..3074
                     /gene="fxsT"
                     /locus_tag="VGT44_14375"
     CDS             519..3074
                     /gene="fxsT"
                     /locus_tag="VGT44_14375"
                     /inference="COORDINATES: protein motif:HMM:NF040586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxSxx-COOH system tetratricopeptide repeat
                     protein"
                     /protein_id="HEV2582038.1"
                     /translation="MQRNEHLRRQRIGRNWRQQDVADQLGTTVTTIQRWERGHQQPSA
                     YFRAKLCTLFGLSAQELGLVETLSSPPESTEVTQTGSAPSEEISLWTVPYARNPHFTG
                     RDDLLQQLGQLLAAEQPGEAMSIQQAALTQTQAITGLGGIGKTQIAIEYAHRARIQGR
                     YRHTIWISAGSSETILTSFVELARLLMPALVEEEKNQRTIVAALLRWLEHCMQPWLLI
                     FDNADDLSLVQPYLPRWGNGRILLTTRAHAVGAFASSLEVDSMGVMEATHLLLRRAHR
                     FSASSPEELDEAIMLVTALAQFPLAIDQAGAYIEETGCSLFAYHELYQRYRHALLARR
                     GRQSTGYPESVATTWSLSFAQVERTNPAAAELLQLCAFLAPDHIPEELLTEGAAHWPV
                     ALCEAVTNPLSFNRMLETLLAFSLVKRLSEDQMLNLHRLVQVVQMERMDAEAQHSWAQ
                     RLVRALHTIFPQETQDVANWPRCQRYLEQVEACDVLIQRHHLLLPEAAELLDRAGTYI
                     REHMWYTQAEPIYRRALAIREQYLGPEHPETATIVHNLAILQRDSGNFVEAEQLYKRA
                     LAIREQHLGPEHPDTANAYSNLANLYLAQKRYREAEPLYRRALAIREQLSGPPQLDSD
                     DQLHTLANFYVTQGRYREAESLYQRILAIREQRLGPLHYRTANILNNLAVLYLSQGRY
                     TEAEPLYRRVLTLREQQFGPTHPHTATSLYNLATLQRDKGNVVEAEQLYQRALGIREQ
                     QLDPEHPTFADCLGGLARLYQKQGKIAQAEALVLRALRIHEQHLGPLHPSTADDLHTL
                     AMLYRDQGKHREAEPLFLRALSVREQAFMSTHLSLAETLFEFALLTCSYLEHT"
     gene            3217..3693
                     /locus_tag="VGT44_14380"
     CDS             3217..3693
                     /locus_tag="VGT44_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV2582039.1"
                     /translation="MNPADKKQALRSFTYGLYAVSCADAGQVNIFTANWLTQVSFEPP
                     LLAVSVENDSKSLPMILRSSVFTVNVLRSGDRELAGRLGKSAIQHPDKLTGVAYDIGE
                     NGCPVLRDALAWVACAVKRSAEAGDSTLLIAEVVDVGILGEGQSLTMAEAGFRHAG"
     gene            3749..4414
                     /locus_tag="VGT44_14385"
     CDS             3749..4414
                     /locus_tag="VGT44_14385"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEV2582040.1"
                     /translation="MQNGSGFSVPAPVPDPNCDFCAPGKLAYVLYETSHFRLAADFAP
                     LVEGHLLIIPKDHYACYGAVPAALDQDLFALKETVRQFFTQYYAPPVFLEHGIFRQTV
                     FHAHLHCFPWGTIEYDMDAGVHEQVVTSQDEVRRWYAQRGHYFYLEDPALALLFPPDV
                     ERYFRIIRGVFMRGMLLRGGKAELRPPQQRLAEGGPLIQAVVEKWQLFQTQGVSYASE
                     SGA"
     gene            4392..5258
                     /locus_tag="VGT44_14390"
     CDS             4392..5258
                     /locus_tag="VGT44_14390"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2582041.1"
                     /translation="MPVSPVRDEYVIAGDLTFHYVQWGEHGRPLVFVHGITAHAFYFQ
                     ALADGLAQRYRVIAYDLRGRGDSDKPEQGYSIPIHAADLAEIIDELELDRPVVIGHSL
                     GAMIGVYFAANYPEKLSKLVLLDGGGNLPWRTVAEQPAWLTTSIGRLGTPVASFDEYV
                     GRLKAVPFLGPSWNAYADLYFAHDVFHQHDGSVISKGYAGGVREDQRYLEEWHPDALF
                     GRIHAPTLLVRAGQGLFSSDDQLLPTAEAERMQQSIAHCQYVNFPALNHYTLGFGLDP
                     AVARVIDDFVKE"
     gene            5265..6116
                     /locus_tag="VGT44_14395"
     CDS             5265..6116
                     /locus_tag="VGT44_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEV2582042.1"
                     /translation="MAIRRVGVIGCGLMGGGIAQTYAQSGYETVVREINQPLLDKGLG
                     RIYDAWEMMASKGKLTQGQVEENRARLRGTLDLEELADCDLVIEAVIENMEEKLRLFP
                     ALDHIVKPGGLLLTNTSSLNVTQMGAVTKRPEMVCGLHFFNPAPVMKLVEIVRTISTS
                     DETVDTVRSFALSLGKTPVIAKDTAGFIVNFLLIPYLLAAIRMLENGMASRDDIDTAM
                     KLGCGYPMGPFTLLDYVGLDTTMWAAEAIYDEYKDPLYAPPPLLRRMVQSGMYGKKSG
                     RGFYEYR"
     gene            complement(6184..6921)
                     /locus_tag="VGT44_14400"
     CDS             complement(6184..6921)
                     /locus_tag="VGT44_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582043.1"
                     /translation="MSFTQDELQSFHTVLEQRLQAHQREIEHAFDERLSEYRRDNEHR
                     LYAMQHELLRAISFKLTEFQGQVEGLLSQQAQQLALASLDQLNEEALANGHQQLEAIE
                     VQTELPWEDLAAVIGQTLDERLNSIKEETRRLLSQQEHQFAQQLHGLRNELAQLIAQA
                     ASNSEQSEGGPALSDLLQGIEHLERVIESLQVVMTANHALLSDRLYSHQQQPVERAHP
                     AGHLRAHLPAEQIRTEDRLTESSIAGS"
     gene            complement(7077..>7630)
                     /locus_tag="VGT44_14405"
     CDS             complement(7077..>7630)
                     /locus_tag="VGT44_14405"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2582044.1"
                     /translation="DLGLAGAKFHPGVQAFYPNDPRFYPLMEKIQALGVPALFHTGTN
                     GLGAGTPGGMGIKLDYTRPIYLDSLAADFPGLTIIGAHPSWPWHEEMIAAMQHKTNLY
                     NDLSGWSPKYIPEALLREAATRLQDRFLFGSDYPFISPQRWMKDFESVKIEFKPEVRE
                     KLLWRNGQKLLEHTAVGQMHFST"
BASE COUNT         1637 a         2152 c         2114 g         1727 t
ORIGIN      
        1 tccagtttgc caaacgtctc gtatgcacga gccatcaact gatcaatctc attgagcttc
       61 gtgacatcgg ttggaaccac cagaacattg tgaccaagtt ctcgagagac ctcttcaagg
      121 gtctcacgat cgcgtcccga aatgacgact ttggctccat tggcatgaaa ttctctagcg
      181 gttgccaggc caatcccact attgccacct gtcactacag caacttttcc tgcaagcgaa
      241 ctcataccac tcttttcctt tcgataggag aacttcctct ctgaaaaagg ataccctgtt
      301 gtgctgcttt ttcagagagc gttatcggtc ttcaaagtga ccttttccgt cgctttaaaa
      361 agaaacaaga acgagaaaaa ccttcacagc gtatcgcaaa ttatccagcg tatcgtatac
      421 tatccaaagg atgacctttt ccctcttgat cttgcaggaa caccagggaa gtaagcttga
      481 gagtcctcaa tcatatgggt agatcagaag gaaatgctat gcagcggaat gagcacttac
      541 gccggcagcg catcgggcgc aactggcgac agcaagatgt cgcggaccag ttaggcacca
      601 ccgtcaccac tattcagcgc tgggaacgag gccatcagca acccagcgcc tacttccgtg
      661 ccaagctctg cacgcttttt ggtctcagcg cccaggagtt aggattggtg gaaacgcttt
      721 cgtcaccacc tgagagcacc gaagtcacac agacggggag cgctccctcc gaggagatct
      781 cactctggac ggtcccgtat gcacgcaatc ctcactttac cggacgtgac gacttactcc
      841 aacaacttgg gcaactgctc gcagcggagc agccagggga ggcgatgagt attcagcagg
      901 cggccttgac gcaaacacag gccattacgg gattaggtgg tattggcaag acgcagatcg
      961 ctatcgaata tgcccatcgg gcacggatac aaggacgcta tcgccatacg atctggatct
     1021 ccgctggaag ctcagagaca atcctgacca gctttgtcga actcgcccgt ctgctcatgc
     1081 cagctcttgt ggaggaggag aagaatcagc gcacgatcgt tgctgctctc ttgcgctggt
     1141 tagaacactg tatgcagccc tggctgctta tttttgacaa cgccgatgat ctctccctgg
     1201 ttcaacccta tctccctcgc tggggcaacg gacgcatcct gctcacgacg agagcacatg
     1261 cggttggcgc atttgcctcc tctcttgaag tcgatagcat gggagttatg gaagcgacgc
     1321 acctcttact ccggcgggcg caccgctttt ctgccagttc gcccgaggag ttggacgagg
     1381 ccatcatgct cgtgactgcg ctcgcccaat ttcccctggc aatcgatcag gcgggcgcct
     1441 acattgaaga aaccggatgc agtctcttcg cctatcatga actctaccag cgctatcgtc
     1501 acgccttgct cgcacggcgt ggcaggcagt ccacagggta tcccgagtcg gttgccacga
     1561 cgtggtccct ctcgtttgca caggtagaac gaaccaaccc ggcggcagcc gaactgttac
     1621 aactctgcgc ttttctcgcc ccagaccaca tccctgaaga actgctcacg gagggtgctg
     1681 cccattggcc tgttgccctg tgtgaggcag tgacaaatcc cctgagcttt aaccggatgc
     1741 tcgagacctt gctggccttc tccctggtga agcggttgtc tgaggaccag atgctgaatc
     1801 tccaccgcct cgtccaggtc gtgcaaatgg agagaatgga cgcagaggca caacatagct
     1861 gggcgcagcg cctggtgcgt gccttgcata ccatcttccc tcaagagaca caagatgttg
     1921 cgaactggcc ccggtgtcag cgctacctgg aacaggtaga agcatgcgat gtcttgattc
     1981 agcgccatca tctcctgctg ccagaggcgg cggagttgct cgatcgtgca ggcacctaca
     2041 tccgtgagca catgtggtat acacaggcag aaccaatcta tcgacgtgct ctggccattc
     2101 gcgagcagta ccttggtcca gaacatcctg agacggccac cattgtacac aacctggcta
     2161 tactgcaacg agatagcggc aattttgtgg aggcagagca gttatacaag cgcgccctgg
     2221 ccattcgcga gcagcacctc ggcccagaac atcctgatac tgcgaacgcc tatagcaacc
     2281 tggcaaacct ctacctggct cagaagcgat accgggaggc ggagccatta tacaggcgcg
     2341 ctctggccat tcgcgagcaa ctctctggtc cgccccagct cgattctgat gaccagttgc
     2401 ataccctggc aaacttctac gtgactcagg ggcgctacag ggaggcagag tcgttgtacc
     2461 aacgcatcct ggctattcgc gagcagcgcc tcggtcctct tcactaccgc accgccaata
     2521 ttctcaataa tctggcggtc ctttacctct ctcaggggcg gtatacagag gcggagccgt
     2581 tgtacaggcg tgttcttact cttcgcgagc agcaattcgg tccaacacat ccccatacgg
     2641 cgaccagcct gtacaatctg gccacgcttc agcgagacaa aggcaacgtt gtggaggcgg
     2701 agcagctata ccaacgcgcc ctgggtattc gcgagcagca gctcgatcca gagcatccta
     2761 ccttcgccga ctgcctgggc ggtctggcaa gactgtatca aaagcaaggg aagatcgcac
     2821 aggctgaagc gttggtcctg cgcgcgctac gcatccacga acagcacctt ggaccgcttc
     2881 atccctccac cgctgatgac ctgcatacac tggcaatgct ctatcgagac caggggaagc
     2941 atagagaggc agagccatta tttcttcgtg cgctctcggt ccgtgagcag gcgtttatgt
     3001 ccacccatct ctcccttgct gaaaccttgt tcgagttcgc tctcttaact tgctcatatc
     3061 tagagcatac ctgagcaggc gtacccgtgt catgagatgg attcccaatc cgctgggaag
     3121 ttgttcccga tccgtcggga ttggctgctt gctctgctcg accttacgtg tgatacactt
     3181 ggtgaaaatc accgttgcaa acaaagggga aagtgattga atccagccga taaaaagcag
     3241 gcgttgcgat ccttcaccta tggactgtat gctgtttcgt gcgccgatgc aggccaggtg
     3301 aacatcttta cagccaactg gctcacgcag gtgtcgtttg aacccccgct gctggctgtt
     3361 tcggtggaaa atgactcgaa atcgctgccg atgatcctgc gcagcagcgt atttacggtc
     3421 aatgtgctgc gttcgggcga ccgtgagctg gcgggcaggc tgggtaaatc cgccatccag
     3481 catcccgata agctgaccgg tgttgcctac gacattggcg aaaatggctg ccccgtgctt
     3541 cgcgatgccc tggcatgggt cgcctgtgcg gtcaagcgaa gcgcggaggc gggcgattcg
     3601 accctgctca ttgccgaagt tgtggacgtt ggcatactgg gagaaggaca gtccctgacg
     3661 atggccgaag cgggttttcg acatgcaggg taaaggaggt ttgtgctaag atagcaatag
     3721 tagcgcacca tgtaaaggag ttatcttcat gcaaaacgga agtggctttt ccgttcctgc
     3781 cccggttcct gatcccaact gcgatttttg cgcccccggc aaattagcct acgtgctgta
     3841 tgaaacgtcc cacttccggc tcgccgcaga tttcgccccg ctcgtggaag gccatctgct
     3901 tattatcccg aaagaccatt atgcctgtta tggagctgtt ccggcggcgt tagatcaaga
     3961 tttgtttgcc ttgaaagaga cggtacggca atttttcacg caatactatg ccccacctgt
     4021 ttttctagaa cacggcatct ttcgacagac ggtcttccac gcgcacctgc actgtttccc
     4081 ctggggtacg atcgagtatg atatggacgc tggcgtacac gagcaagtcg ttacttccca
     4141 ggatgaagtg cgcaggtggt atgcacagcg cggacactat ttctacctgg aagacccggc
     4201 gcttgcgcta ctgttccctc ctgatgtgga acgctacttt cgtattatca gaggcgtctt
     4261 catgcgcggt atgctgctgc gcggcggcaa agccgaactt cgcccgccgc agcaacgtct
     4321 cgccgaggga ggtcctctta tccaggctgt cgtagaaaaa tggcagcttt tccagacaca
     4381 aggagtatcc tatgccagtg agtccggtgc gtgatgaata tgtcatcgcg ggagacctga
     4441 ccttccacta cgtccagtgg ggtgagcatg gtcgacccct ggtcttcgtg catggtatca
     4501 ccgcccatgc cttctatttt caagcgctgg ccgatggtct agcgcagcgc taccgcgtta
     4561 tcgcctatga cctgcgtgga cgaggggaca gcgacaagcc ggaacagggc tacagcatcc
     4621 caatccatgc cgccgatctt gccgagatca tcgacgagtt ggagctggac cgtcccgtcg
     4681 ttatcggtca ttcgctgggg gccatgatcg gcgtctattt cgccgccaat taccccgaga
     4741 agttgagcaa gctggtgctg ctcgatggcg gcggcaactt gccctggcgc acggtcgcgg
     4801 agcagcccgc ctggttgacc acatcgattg gtcgcctggg aacgccggtc gcgtcatttg
     4861 atgagtacgt tggacgtttg aaggctgttc cctttctcgg cccctcctgg aatgcgtatg
     4921 ccgatctgta ttttgcgcat gatgtctttc accagcatga cggttccgtt atctcgaaag
     4981 gctacgctgg tggtgtgcgc gaggatcaaa gatatctcga agaatggcat cccgacgcgc
     5041 tgtttggtcg tattcatgcg cctacattgc tcgtgcgtgc cggtcagggg ctattctcct
     5101 cggacgacca gttacttccg actgcggaag ctgagcgaat gcagcagtct atcgcgcact
     5161 gccagtatgt gaattttcct gcgctgaacc actatacgct cggttttgga ctcgacccgg
     5221 cggtggcgcg cgtgattgat gatttcgtaa aggagtaata gtcgatggct ataagaagag
     5281 tcggcgtcat cggttgcgga ttgatgggcg gtgggatagc gcagacatac gcgcagtcgg
     5341 gttatgagac agtcgtacgc gagatcaatc aaccgctgct cgataaaggg ttaggacgta
     5401 tttacgatgc ctgggagatg atggcgagca aaggcaagct cacccaggga caggtcgagg
     5461 aaaatcgcgc ccgcctgcga gggacactgg atctggagga actggccgat tgtgacctgg
     5521 tgattgaggc ggtcatcgag aatatggagg aaaagctgcg cctcttcccc gcgctcgatc
     5581 atatcgtcaa accgggcggg ttgctgctca ccaatacatc gtcgctgaac gtgacccaga
     5641 tgggcgcggt gacgaagcgg ccagagatgg tttgcggcct gcacttcttc aacccggcgc
     5701 ccgtcatgaa actggtcgag atcgtgcgca ccatttcaac ctcggatgag acggttgata
     5761 ccgtgcgcag ttttgcgctc tcgctgggga aaacgccggt gatcgcgaaa gacaccgcgg
     5821 gattcatcgt gaatttcttg ctcattccct acctgctggc cgcgatacgt atgttggaga
     5881 acggcatggc cagccgcgac gacatcgata ccgccatgaa gctcggctgc ggctacccga
     5941 tgggaccgtt cacactgctc gactatgtcg gccttgatac gaccatgtgg gcagccgagg
     6001 ccatctacga cgagtacaaa gacccgctct acgcgcctcc cccattgttg aggcgcatgg
     6061 tgcaatcagg catgtatggc aagaagagcg gtcggggctt ttacgagtat cgttaggcgt
     6121 tgaggtggga tggacgaaac gtcttgaatg atctatccta cctcaacgaa catgacgtat
     6181 caattacgat ccagcaatag aggattcggt aaggcgatcc tcagtcctga tctgctcggc
     6241 ggggagatgc gcgcggagat gcccggcggg gtgggcgcgc tcaaccggct gctgctgatg
     6301 ggagtagagg cgatccgaaa gcaaagcatg attggcggtc atgaccactt gcagcgactc
     6361 aatcacgcgt tcgagatgtt cgatgccttg caggagatcg ctcagagcag ggccaccctc
     6421 ggactgctct gaatttgaag ctgcctgcgc tatcaactgt gccagttcat tccgcaatcc
     6481 gtgcagttgc tgcgcgaact ggtgctcctg ctggctcagt aggcggcgag tttcttcctt
     6541 aatggagttg agccgctcat ccagcgtttg gccgatcaca gcagccaggt cttcccaggg
     6601 caattctgtc tgcacctcga tggcttcgag ctgctgatgg ccgttggcca gggcttcctc
     6661 gttcaactgg tcaagcgacg ccagcgccaa ctgttgcgcc tgctggctca ataatccctc
     6721 gacctgtcct tgaaactcag tcagtttaaa agagattgcg cggagcagtt catgctgcat
     6781 ggcatacaag cggtgttcgt tatctcggcg atattcgctc aggcgctcgt caaacgcatg
     6841 ttcgatctcg cgctggtgcg cctgcaagcg ctgttctagt accgtatgaa aggattgtag
     6901 ctcgtcttgt gtgaacgaca tggatcgctc tccttgcttt cctaccagtt atcgaggtaa
     6961 tacgcctggt aagtataaga gtcaggcccg tcacctgtca atttatggag catttcaagc
     7021 caatgttggc tatcatgaca tagtgatatg gcttcccttc aaacgtattt atgctctcac
     7081 gttgaaaagt gcatctgccc aacggcggta tgctcaagca gcttttgtcc attcctccac
     7141 aaaagcttct cgcgcacctc cggcttgaat tcaatcttta ccgactcaaa gtccttcatc
     7201 cagcgctgcg gcgagatgaa agggtagtcc gagccaaaaa ggaaacgatc ttgcaggcgt
     7261 gtggcagcct cgcgcagcaa agcttcagga atatacttcg gtgaccagcc ggagagatcg
     7321 ttatatagat tggtcttgtg ctgcatcgcg gcgatcattt cctcgtgcca gggccaggaa
     7381 gggtgcgcgc cgatgatggt caggccgggg aaatcggcgg ccagtgaatc taggtagatg
     7441 gggcgcgtat aatcgagctt gatgcccata ccgcccggcg tccccgcacc caggccattc
     7501 gtgccggtgt ggaagagcgc cgggactcca agcgcctgaa tcttctccat caaggggtaa
     7561 aagcgtggat cgttcgggta aaacgcctgc acgccggggt ggaatttcgc gccggccagc
     7621 cccaggtctt
//
LOCUS       DASZAK010000815         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_695688, whole genome shotgun sequence.
ACCESSION   DASZAK010000815 DASZAK010000000
VERSION     DASZAK010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695688"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..2357
                     /locus_tag="VGT44_14410"
     CDS             219..2357
                     /locus_tag="VGT44_14410"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV2582045.1"
                     /translation="MSTLRHSTGQPQGPSRAEASSPTPLHSDPAPTDPAALAPTLTTY
                     RVAAIQYEPTLGEKEKNVGDLLRLVEEAAKHDARLIVVPEMATTGYCWESRSEIALHV
                     EPIPGPTTDRFQRLAATYNCFIAIGLPEVDPATDVYYNSLVLLGPEGIVGTYRKIHSY
                     MSEPRWARDGDLGLPVWDTPLGRLAPMICMDAGYFEAARIPALLGADILLFSTNWFDE
                     KCPSSWWMARAFENRLYLIAANRYGVERGIQFSGGSCILNPDGSIQHYLDNGEGIVYG
                     EVDLSCSRNKSWGPPDAEPVGDCLADRRPSEYMTLVNNTYLWEPLRYHGLYDLAALPP
                     GQLSCVAIIQTDLQTFPRAEAPTINRGATRPYPAPPPFDIRSSLQFLRDMVKTFINDH
                     SPACPDVLVLPELMLPGPVPRIAGTPVPQTGSQEIAHFQSGAISVPGPETDALVELAN
                     ELQISFVLGVAERVNEGQPQGSPPLRARYYNTVLLIDPEGIYGTYRKLHLTQQDRRWA
                     SSGDLGLPTFDTPVGRIGLATGYDVLFPETLRILAGKGADLICAPTLLNFPDPIGLGP
                     TSIPYGPQIAPQEYDPVHYLIWRVRAAEHNVYLALANWSGQSDGLHANGNSGIFAPDC
                     STYPWSEVIAEEGEPGLTMMTIDTREQRTGRRSTTLSPSYTPGDVVGSLTGELAYDIR
                     DTIPGNIARSKPLLRKRMPFWYLDLIRIPR"
     gene            2391..3155
                     /locus_tag="VGT44_14415"
     CDS             2391..3155
                     /locus_tag="VGT44_14415"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEV2582046.1"
                     /translation="MRLANKVALITGSAGGMGKAAAELFAREGASIVVTDIATDQGEE
                     TARGIRDAGGNAIFVKANVANEDEVKRLVGAAIDAFGHIDVLYNNAGIMPTDDGSVTD
                     ITEATWDKVMDVNLKSAFLCSKYTIPHMVKQGKGSIINVASFVAFLGCTVPQDAYTVS
                     KGGMLSLTKSLAVQYGRHGVRCNAICPGPIETPLLRVLWTSEEARNLRLNRIPLGRFG
                     EANDIVYMALYLASDESSWTTGAWLVVDGGITSNYF"
BASE COUNT          783 a         1059 c          853 g          696 t
ORIGIN      
        1 taaattgggg ttctacccag caagatccta ttccacaggc tagtgacgcc gatggtcaat
       61 tcggatatat ctatccgcca gatagagcac ccaatccggg agatgggtat ccgcaagggt
      121 ctccgcctcg ggcggaggca agctccccca ctccactcca cctcgctccc gcccctacgt
      181 accccaaagg tgggggcctg cgaaagggat aagccaagat gagcacgtta agacattcca
      241 cagggcaacc gcaagggccc tcacgggcag aggcaagctc ccccactcca ctccactccg
      301 atcccgcccc tacggacccc gccgcccttg cccctacgct caccacgtac cgggtcgcag
      361 caattcagta cgagccaacg ctgggtgaaa aggagaaaaa cgtaggcgat cttcttcgtc
      421 tggtcgagga ggctgcaaaa catgacgcac gcctcatcgt cgtgccagaa atggcgacca
      481 ccggctattg ctgggaatct cgcagcgaaa tcgcccttca tgttgagcct atccctggtc
      541 caaccaccga ccgcttccag cgactcgctg ccacctacaa ctgcttcatt gctattggcc
      601 ttcccgaggt agacccggcc acggacgtgt actacaatag cctggtccta cttggcccag
      661 agggcattgt cggcacctat cgcaaaatcc attcctatat gtcagaaccg cgctgggcac
      721 gtgacgggga cttgggtttg cccgtctggg acaccccgct cggtcgcctg gcgcctatga
      781 tctgtatgga tgccgggtat ttcgaggccg cacgcattcc cgcattgctt ggtgcagaca
      841 tcctgctctt ctctacgaat tggttcgatg agaaatgccc cagtagctgg tggatggctc
      901 gcgcctttga gaatcgcctg tatttgattg ctgcgaatcg ctacggtgta gagcggggca
      961 tacagttcag tggtgggagc tgcatcctca atccagatgg ctctatccag cactatctcg
     1021 acaatggtga aggcattgtc tacggtgagg tcgatctgag ctgcagccgt aataaaagct
     1081 ggggcccgcc tgatgcagag cccgttggtg actgccttgc cgatcgacgc cctagcgagt
     1141 acatgacatt ggtcaataat acttacctgt gggaaccatt acgctaccac ggtctgtatg
     1201 acctggcggc gcttccccct gggcaacttt cttgtgtcgc cattattcaa accgacctgc
     1261 aaacgtttcc aagagcggag gctcccacga taaatcgggg agctacacgc ccctaccctg
     1321 cacctccacc attcgacatt cgatcatcgc tacaatttct acgcgatatg gtcaagacat
     1381 tcatcaacga ccattccccg gcatgccccg acgtgctggt gctgccagaa ttgatgctac
     1441 ccggtcctgt gccacgtatt gcaggcacac ctgtccccca gacagggtcc caggagattg
     1501 cccattttca gagtggggcc atctcggttc caggaccaga aaccgacgca ttagtggaac
     1561 tagccaatga acttcaaatc agcttcgtgc ttggcgttgc ggagcgggtg aacgaagggc
     1621 aaccgcaagg gtccccgccc ctacgggcca ggtactacaa tacggtgctg ctgatcgacc
     1681 ctgaaggcat ctatggcacc tatcgcaaac tccacctgac acagcaggac cgccgttggg
     1741 ccagctcagg cgatctggga ctgccaacct tcgacacacc ggtaggccgc atcggactgg
     1801 caaccggcta cgatgtactc ttcccagaga cactgcgtat cctggcagga aagggagcag
     1861 accttatatg cgcccctaca ctgctgaact ttccagaccc gattggcctt ggaccaacga
     1921 gcattcccta tggaccacaa attgcaccac aggaatatga tcccgtccac tacctgatct
     1981 ggcgtgtgcg agctgccgaa cataacgtct acctggcgct ggcaaactgg agcggtcaat
     2041 ccgatggact gcatgccaat ggcaatagcg gcatctttgc acctgactgc tctacctacc
     2101 cctggtcgga agtcatcgct gaagaaggtg aaccaggctt gacgatgatg accatcgaca
     2161 cacgcgaaca gcgcacaggc cgccgttcga ccacgctatc gcccagctac acccccggtg
     2221 atgtcgtcgg ctcgctcacc ggcgagctag cctatgatat ccgcgacacc atccccggca
     2281 acatcgcacg cagcaaacct ctgctgcgca aacgtatgcc tttctggtat cttgacctga
     2341 tacgaatccc acgatgaatc ggggatatac ctagaggaga ctcacatctt atgcgcttag
     2401 ccaacaaagt tgcacttatc accggttccg caggtggcat gggcaaggcc gccgccgaac
     2461 tatttgcgcg tgaaggggcc agcatcgtcg tcaccgacat tgcaaccgac cagggcgaag
     2521 agaccgccag aggcattcgt gatgcaggcg gcaacgccat ctttgtcaaa gccaacgtcg
     2581 ccaacgaaga tgaagtcaaa cgccttgtcg gcgctgccat cgacgcattt ggtcatatcg
     2641 acgtccttta taacaacgca ggcatcatgc ccaccgacga tggtagcgtg accgacatca
     2701 ccgaggctac gtgggacaag gtcatggatg tcaacctcaa gagcgccttt ctctgctcga
     2761 aatacaccat cccacacatg gtaaagcagg gcaaaggctc gatcatcaac gtggcctcat
     2821 tcgtcgcctt cctgggctgc acagtgcccc aggacgctta taccgtctcc aaaggcggca
     2881 tgctctcgct caccaaatct ctcgcagtac aatatggccg tcatggcgtg cgctgcaacg
     2941 ccatctgtcc aggtcccatc gaaacacccc tgttgcgtgt cctgtggacc agcgaagaag
     3001 cccgtaacct gcgcctgaac cgcatcccac tcggtcgctt cggcgaggcc aacgacatcg
     3061 tctacatggc gctctacctg gcctccgacg agtcctcctg gacaacaggc gcatggctcg
     3121 tcgtcgatgg cggcatcaca tcgaactatt tttagatgat cctgaagata ttccctacgt
     3181 cttatcctac ttacctgcct ggtcggtttc ttcctgaaag tgtaaggacg tgcacatata
     3241 atgcacactg ccatgcctac atgcccaaaa aagtgacaaa agccacatat tcggatatac
     3301 tattggcagt gcttttaccg ttccaggtgg aaaaaacaat gcaaactaca acaactgaaa
     3361 ggctaacacg cggctacctt cagaaattgg a
//
LOCUS       DASZAK010000816         2370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_269863, whole genome shotgun sequence.
ACCESSION   DASZAK010000816 DASZAK010000000
VERSION     DASZAK010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269863"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="VGT44_14420"
     CDS             complement(<1..875)
                     /locus_tag="VGT44_14420"
                     /inference="COORDINATES: protein motif:HMM:NF012537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEV2582047.1"
                     /translation="MLEQQQPTTKAEQGPGSQKMTQLEGIRQKVFMDRYSLKDPNGQP
                     LEFYPEQLWARVAKGIAAVESTEELRAHWEKRFYEALYGFQFVPGGRILAGAGSGHQV
                     TFYNCMPPDQEVLTADGYRPISEIKLGDLVVTHRNRLRPVLHKFERETAETLYIIRPK
                     KVGYDDLRVTGDHKVYVVRSEWVNTYRSRDGLRLQHEPDWIPAKEIKPGDYVAIVAYN
                     SDECPSDFEYDSLKWVRVDEIAIEDYAGIVLDIEVEEDHSFISAGIVVSNCYVIPSPE
                     DSRQGILDNLKVMTE"
     gene            1147..>2370
                     /locus_tag="VGT44_14425"
     CDS             1147..>2370
                     /locus_tag="VGT44_14425"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV2582048.1"
                     /translation="MRAEILSCGTELLLGHITDTNATYLAQSLAALGIDLYFVSQVGD
                     NQGRIVETLRRAWERSDLIIMTGGLGPTEDDLARESISALLGETMQVDPRLEAELRGM
                     FATRRSIMPERNIKQATLIPSAQALPNPRGTAPGWWVEKDGHIIVAMPGVPREMYQMW
                     EREVVPRLSKYTGGLIFTRILRVWGLGESTVEERLQALLHNTNPTIATYAKSDAVDVR
                     ITAKAETREQAEQLVTEMEAQARQALGYHVFGVDKQTLQSVIGTRLVERRQSLAVMES
                     LTGGLLASTITDEADSSRYFVGGIVSYSAELKIQMGVPREVIDRYGVISEETARAMAH
                     AVRERLGADYGLGITGVAGPEKQEDKPVGTVYIAVEGPEGVVTGMGPGWRASREDTKR
                     LSVMAALNLLRLHLEG"
BASE COUNT          536 a          625 c          648 g          561 t
ORIGIN      
        1 atctccgtca tcaccttcaa gttatccagg atgccctggc ggctgtcttc agggcttggg
       61 atgacgtaac agtttgagac cacgataccg gctgaaatga acgagtgatc ttcctcgacc
      121 tcaatatcca gcacgatgcc agcataatct tcaatagcaa tttcatcgac ccgcacccac
      181 ttgagactat catactcgaa gtcggaaggg cactcgtcac tgttatatgc gacgatggca
      241 acataatcgc caggtttaat ctctttggcg ggaatccaat ctggctcatg ctgcaaccgc
      301 aggccatcac gcgatctata cgtgttcacc cattctgagc ggacaacgta tactttgtga
      361 tcgcctgtca cacgcaaatc atcgtagccc actttcttgg gtcggatgat gtagagtgtt
      421 tccgcagttt cgcgctcaaa tttatgtaat accggacgca gacgattgcg atgcgtcacc
      481 acaaggtcgc caagcttgat ttcggaaatt ggtcgataac catctgctgt aagcacttcc
      541 tggtcaggcg gcatacagtt atagaatgtt acctggtgcc ctgaccccgc tcccgcgaga
      601 atgcgaccac ccggtacaaa ctgaaagcca tacagcgcct cgtaaaaacg cttttcccag
      661 tgcgcacgca gctcttcagt cgattctaca gcggcaatgc ccttcgctac ccttgcccag
      721 agttgttcgg gataaaattc taagggctgt ccgttggggt ccttgagaga atatctgtcc
      781 atgaaaactt tctggcggat cccctccaat tgcgtcattt tttgggaacc aggaccctgt
      841 tcggctttag tggtgggctg ctgttgctcc aacattggtt cattactcct tccatacttc
      901 agtcatgtat gggctttgaa ataaatttgg ttgttttaat ctccgagaca cagtatactt
      961 gaaaaagctt gctttttcaa gggttttggc acaacatatt gattttctag cgttctgtgg
     1021 cacaacatat tgattatgga tgagtataag catggacaca acctgagcac agccaatgta
     1081 atcctcataa gtgatatgat atggctattg gaatcaaact gaacttatgc gcaatctgga
     1141 ggatgcatgc gtgccgaaat tctctcgtgt ggcactgaat tgttgttagg ccacattacc
     1201 gataccaacg ccacttatct tgctcagagc ctggcagcgc tggggattga tctctatttt
     1261 gtttcacagg tgggagataa ccagggacgc attgttgaga ccttgcgacg ggcctgggag
     1321 cgatctgact tgattatcat gacgggtgga ttgggtccaa ccgaggatga tcttgcccgc
     1381 gagtctataa gcgccttgct gggtgagacg atgcaagtag acccaagatt agaggccgag
     1441 ttgcgcggaa tgtttgctac gcgtcgtagc ataatgcctg agcgaaatat taaacaggca
     1501 actttgattc cttctgcgca agcgctgcca aatccgcgtg gaactgctcc tggttggtgg
     1561 gttgagaagg atgggcatat catcgtcgcc atgcccggtg tgccgcgcga gatgtaccag
     1621 atgtgggagc gcgaggtcgt accgcgcctg agcaaatata ccggcggcct gatcttcacg
     1681 cgcatcctgc gcgtctgggg cctgggcgaa tcgactgttg aggaacgcct gcaagctctg
     1741 ttgcacaata ccaaccctac gattgcgacc tatgccaagt cagacgccgt tgatgtgcgc
     1801 atcacggcca aagccgagac tcgcgagcag gccgagcaac tggtcacgga aatggaagcg
     1861 caggcacgcc aggcactcgg ctaccacgtc ttcggcgtag ataaacagac cctgcaaagt
     1921 gtcatcggca cccgcctggt tgaacgccgg caatcgctgg cagtcatgga atcgctgacg
     1981 ggcggtctgc tggccagcac gatcaccgac gaggccgaca gttcgcgcta cttcgtcggt
     2041 ggcattgttt cctacagcgc tgaactcaaa atacagatgg gcgtgccgcg ggaggttatc
     2101 gaccgctatg gggtcatcag cgaagaaacc gcgcgcgcta tggcccatgc cgttcgcgag
     2161 cgcctgggcg cggattacgg cctgggcatc accggtgtgg ccgggccgga gaagcaggaa
     2221 gacaagccgg tcggcaccgt ctatatcgct gtcgaggggc ctgagggtgt tgtgaccggc
     2281 atgggtccag gatggcgcgc cagccgcgag gataccaagc gcctctcggt catggccgcg
     2341 ctcaacctgc tgcggctgca tttggaaggg
//
LOCUS       DASZAK010000817         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_55292, whole genome shotgun sequence.
ACCESSION   DASZAK010000817 DASZAK010000000
VERSION     DASZAK010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55292"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..2041)
                     /locus_tag="VGT44_14430"
     CDS             complement(272..2041)
                     /locus_tag="VGT44_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582049.1"
                     /translation="MSRFLTRKSAVVLILALALFASLPLAASAQQMSHISGGSSSGAT
                     NIHPICKKIGTKLELSSGARMWCFGAQTSGSSSAARSMLMRNITYGSNIDAANPAEDV
                     SPSGVQAFGQAETSIGSVGPYVVEAWNDATGFFSPCPSPLSKEELTGFAFSSDGGKTF
                     QDEGGLPNNGCASHIYAGDPSVEAWQPGGQAYFYISSLFPSSTPFGLNYLALAACQAI
                     STLITCGQPVIVASSSQCQVFPPFQFCSFLDKDFLSIDPVRGRLYMSYTDFRFDGAGQ
                     IDLAVCDIGTSSGGTGPLGGTAMKPICNNGNAHSLPNGAGTRPYFTVAPNDPQFCENE
                     GAYPSVDVATGDVYVAYEHNWATNIFGPPGCFGSPTMNVMNYIPFKCLATLTLSASPC
                     SKPAASNAVSIISLDAAFIPGYNRFPMNDFPRLAVSDPAGVVSMVWNDGRFDSAGDIL
                     LYSFKLGTLAGIQAAGPVVINSSTGGWHMMPALRNTDADGDLNISFYGRASANTDVTD
                     VLAATDVSPTTASSPASNVLVTTMPSAWSFVSADSVPNFGDYSDNYVQAQPAAPFTTQ
                     QLFVAWTDGRTGVPQPFNDSESV"
     gene            complement(2296..>3999)
                     /locus_tag="VGT44_14435"
     CDS             complement(2296..>3999)
                     /locus_tag="VGT44_14435"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV2582050.1"
                     /translation="PNAGLDFPVPDPLYIYDQFSYVTSHFQRREAGYTANGGHDQFAA
                     YWAQEMATNLAGFGAQIKRDPFPIHGWRERSAVLPAFNMEVTVPGLTHPEQEIIVGCH
                     YDGKADSTESAFDDTSGCAYELGVGKAMGDYWRSQHVYPARTVRFVIFDAEEQGLFGS
                     FHYLDSTINGDLPNVVAMFNEEQSGINYPARFLGRAANPFMPDYIDVTPLQDNAAYPG
                     RIHLTPTQRANVIRFRALWQQAIPAVFAQFQAVGYQSLDYYDSNNQNLSQPIFTPGQQ
                     NNMHIQDDPSSNSDQVPFIYAGLPVVTVTGDQTYYDPNPPVWAYPYDLPEDTLSLMNT
                     YTSGTTRPSPALALALTLPAMLTTWMLNQPDMLGQASADGNPIAAISDIGQSVAGQTI
                     ALDAKASYDPTNPGSTLTYAWNFGDGVTVTGVSVNHTYLRPGSYMLSLTVTSPDGRRT
                     ISKSITVGTAPVYYSNPYSPLGGTNRPNPAVTIPSPNNSLPAQPPLNPPMITAQTPTV
                     TAEPTTSALATPIVTTTPSTASSPTDTSPLALILGLLAAVILLVALTLIAIALYRIRY
                     K"
BASE COUNT          888 a         1025 c         1247 g          839 t
ORIGIN      
        1 cggtcgcaat accgctgagc ctccgaatac ggtctctcgg cttgacccta cgcccggttc
       61 ttcgatagag gtcggcgcga cggtcaacat ctatgtctgg aacctgggcg caatccctac
      121 ggcatcacca actccataga gcgaatagcc tgctcgttca taaaatcctg gggcttgaaa
      181 gtcacatggt gttgctcgtc aaacaaacac acaaaagcga gggtcaggta ttccatgagg
      241 aacgcctgac cctcgctgct ctcatgcagg gctagactga ctcgctgtcg ttgaacggtt
      301 gtggaacgcc ggttcgtcca tcggtccacg ccacgaaaag ctgctgcgtg gtgaagggcg
      361 cggccggttg agcctgaacg tagttatccg aatagtcgcc aaagttcggg acggaatccg
      421 cgctgacgaa agaccaggcg ctgggcatgg tcgtcaccag cacgttgctc gcgggcgaac
      481 tggcggtagt gggactgaca tcagtggcag ccagcacatc cgtcacatcc gtattcgccg
      541 acgctcggcc atagaacgag atgttcaggt cgccatccgc gtcggtgttg cgcagcgcag
      601 gcatcatatg ccacccgccg gtgctgctat tgatgaccac cgggccggca gcctggatac
      661 cggcaagagt gccgagcttg aagctgtaga gcaggatatc gcctgccgaa tcgaaacgcc
      721 cgtcgttcca gaccatactg accacccccg ctggatcgct gacggccagg cgtgggaaat
      781 cgttcatggg aaagcgatta taaccgggga tgaacgcagc atccagcgag atgatgctga
      841 ctgcattcga ggccgccggt ttgctacagg gtgatgctga cagcgtgagc gttgccaggc
      901 atttgaaagg gatgtagttc atgacgttca tcgtgggcga accgaaacaa cctggaggtc
      961 cgaagatgtt ggtagcccaa ttgtgttcgt aagcgacata gacatcgccg gttgctacat
     1021 caaccgatgg gtatgcgccc tcgttctcac agaattgagg gtcattgggg gctaccgtaa
     1081 agtacggacg ggtgcctgct ccattgggga gtgagtgcgc gttgccatta ttacagatgg
     1141 gtttcatagc tgtgccacca agcggacccg ttccaccact ggatgtgccg atatcacaga
     1201 ccgccagatc gatctgtccc gcgccatcga atctgaaatc ggtataggac atatagaggc
     1261 gaccgcgtac cgggtcaatc gacaggaaat ctttgtcaag gaagctacag aactggaatg
     1321 gagggaatac ctggcattgc gaactggagg caacaatgac cggctggccg caggttatca
     1381 gcgtactaat cgcctggcac gcggccaggg ccaggtagtt gagtccaaag ggggtcgaac
     1441 tgggaaaaag gctgctgatg tagaagtatg cctggccgcc gggttgccag gcctcaacac
     1501 tcggatcgcc agcatagatg tgcgatgcgc agccgttatt gggcaaaccg ccctcatcct
     1561 ggaaggtctt gccgccgtcg gacgagaatg cgaagcccgt tagctcttct ttagacagag
     1621 gtgaagggca gggagagaag aacccggttg cgtcgttcca ggcttcgacg acgtaaggtc
     1681 cgacactgcc aatggaggtt tcggcctgcc caaaagcctg aacccccgat ggcgaaacgt
     1741 cttcggctgg attggcggca tcgatgttgg acccataagt tatgttgcgc ataagcatac
     1801 tgcgcgccgc cgaactgctg ccgctcgttt gagcgccgaa acaccacatc ctggcgccgg
     1861 atgatagctc taattttgtg cctatctttt tgcaaatggg gtggatattc gtcgcgccag
     1921 acgaactccc gccagagatg tgggacatct gttgagcaga tgcggcaagc ggcaagcttg
     1981 cgaagagagc aagagccagg atcaaaacta ctgcgctctt acgtgtgaga aatcgactca
     2041 ttttacaaaa catctcctga acttttctct tctgcgtcgc agcgcgcggg cgtcatgcat
     2101 ccttttgatt gaacgaccag cacgaagcgc ggttacgcag aagcatagcc gataatagag
     2161 agtcgccatg ataccgagtg aaaccggtgt aaagatgtgt cgcgatgtgg agagtggagc
     2221 tttcttgcaa aaaatcccat agtaataaac acggatcagc tcaaaattga cggatgagaa
     2281 ctgcgtaatc gagggctatt tgtagcgtat gcgatagagg gcgattgcga tcagggtgag
     2341 cgcaacgagc aaaatgactg ccgcgagtag tccgagaatg agcgcgagcg gtgaagtatc
     2401 cgtagggcta ctcgccgttg atggagtggt agtcacgata ggtgtggcta acgcgctggt
     2461 ggttggctcg gctgtcactg ttggagtttg tgcagttatc atcggtggat tcaagggtgg
     2521 ttgggcgggt agagagttat tcggagaggg gatggtgact gccggattgg gccggttggt
     2581 accgccgagc ggagagtaag ggttgctgta gtataccggc gctgtgccga ccgtaatgga
     2641 cttgctgatg gtacgtctgc cgtcaggcga agtgacggtg agcgaaagca tatagcttcc
     2701 tgggcgcaga taggtatggt tgacgctgac gccggtaacg gtgacgccat ccccgaaatt
     2761 ccaggcatac gtcagagtac tgccgggatt ggttgggtca taggatgctt tcgcatcgag
     2821 tgcgatagtt tgtccggcga cgctttgccc gatatcgctg atggccgcta tcgggttgcc
     2881 atcggcgctg gcctgcccaa gcatatcggg ctggttgagc atccaggtgg tgagcatggc
     2941 gggcagagtg agagccaggg ccagcgcggg cgaaggccgc gtcgttccgc tggtgtaagt
     3001 gttcatgagc gagagcgtgt cttccggcaa atcataggga taggcccaca cgggtggatt
     3061 gggatcgtag taggtctgat caccggtgac ggtcacaacc ggcagcccgg catagataaa
     3121 cggcacctgg tcgctattgc tggatgggtc atcctggata tgcatgttat tctgctgacc
     3181 tggcgtgaag atgggttggg ataggttctg attattgctg tcgtagtaat cgagcgattg
     3241 atagccgacg gcctggaatt gcgcgaagac ggcagggatg gcctgctgcc acagcgcgcg
     3301 aaaacggatg acgttcgcgc gctgcgtggg tgtaagatga atgcgccctg gataggcagc
     3361 attatcctgc aagggtgtca catcaatgta atccggcatg aaaggattgg ccgccctgcc
     3421 cagaaagcgc gccgggtagt tgatgccgct ttgttcttca ttgaacatgg ccaccacgtt
     3481 gggtaagtcg ccattgatgg tgctatcaag gtagtgaaac gagccgaaaa gcccctgttc
     3541 ctcggcatcg aaaatgacga aacgcaccgt gcgcgccggg tagacatgct gactgcgcca
     3601 gtaatcgccc atagccttgc ctacgcccag ctcgtaggcg cagccactgg tatcgtcaaa
     3661 ggccgattcg gtcgagtccg ccttaccgtc gtagtggcag ccgacaatga tctcctgctc
     3721 tggatgagtg agacccggca ccgtgacttc catattgaac gctggcaaca ccgcgctgcg
     3781 ctcgcgccag ccgtggatgg ggaaaggatc gcgcttgatc tgcgctccaa agccggccag
     3841 gttggttgcc atctcctgcg cccagtacgc cgcgaactgg tcatgtcccc cgttggccgt
     3901 gtaaccagcc tctcgtcgct gaaaatgcga ggtgacatag gaaaactggt catagatgta
     3961 caatgggtcg gggacgggaa aatccagccc cgcgttcgg
//
LOCUS       DASZAK010000818         1575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_625938, whole genome shotgun sequence.
ACCESSION   DASZAK010000818 DASZAK010000000
VERSION     DASZAK010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_625938"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1372)
                     /locus_tag="VGT44_14440"
     CDS             complement(<1..1372)
                     /locus_tag="VGT44_14440"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HEV2582051.1"
                     /translation="MKHLPWHAPLTEWPEHGVAALTIRRGESRHPVLFVECEGIRYAI
                     KETTPHMAEREIRNLHEIEFRGIPTLSPVGTVAVAAQPILLDSGEEGVVPQYISGDRG
                     YTVTRLASRVIPHALLFRLPLSRKTKQRLLGAVAVLMVELHEHGIYWGDPSLANLLIR
                     IDGRSILGIMADAETAELFPQAVEEGLRQQDLESFHESLIWQAEDLRQGRGAGEEVEI
                     LEESDYRYFYRRYSWLRREHARLHAVIAMPTFIQMERFLDHLNRLGFSLLSVNEVVLS
                     PFVSILPGWYARRLRELLGIEIPRKYARRFYNMILGHQAQMSEARASEVTIEEAAQDW
                     YQRYHLPTVLLLRQTLTSNQDPLEAYFAIMRHKWKLSEQAGREVPLDEAVIDWAMQQA
                     GKGTLGTFDPADIASWWRTRKSAAQALEPPMIEREKLEPLLSENEQPLVRLPSAQLED
                     ALQEHLE"
BASE COUNT          296 a          483 c          458 g          338 t
ORIGIN      
        1 tttccaggtg ttcctgtagc gcatcttcca gttgcgcgct aggcagccgc accaggggct
       61 gctcgttctc ggagagcagc ggctccagct tttctcgctc gatcatgggc ggttccagcg
      121 cctgcgccgc tgatttgcgc gtcctccacc acgaagcgat atcggcaggg tcgaacgtgc
      181 caagtgtgcc tttgcccgct tgctgcatcg cccaatcgat gacggcctcg tccaggggaa
      241 cctcacgccc ggcctgctcg ctcaacttcc acttgtggcg catgatggcg aaatacgcct
      301 ccagcgggtc ctggttgctc gtcaatgttt ggcgcagcag cagcaccgtc gggagatgat
      361 agcgctggta ccagtcctgc gcggcttcct cgatcgttac ttcgcttgct cgcgcctcgc
      421 tcatttgtgc ctggtggccg aggatcatgt tgtagaagcg ccgggcatac ttgcgcggaa
      481 tctcgatgcc cagcaactca cgaaggcggc gcgcgtacca acccggtaga atgctcacga
      541 aaggtgacag aacgacttcg ttcacgctca gcaaagaaaa tcccaggcgg ttcaggtgat
      601 ccaggaagcg ttccatctgg ataaatgtcg gcatagcgat gaccgcgtgc aggcgcgcat
      661 gttcgcggcg cagccagcta tagcgccgat aaaagtagcg ataatcgctc tcttccagga
      721 tttccacctc ttcgcccgcg ccgcgcccct gtcgcaagtc ctcggcctgc cagatcagcg
      781 attcgtggaa tgactccagg tcctgctggc gcaatccctc ttccaccgcc tgcggaaaca
      841 gttcagcggt ttcggcatcg gccatgatcc ctagaatgct gcggccatcg atgcggatca
      901 gcaagttggc cagtgatgga tcgccccaat agatgccatg ttcgtgcaac tccaccatca
      961 acaccgcgac ggctcccagc aggcgctgtt tggtcttgcg cgaaagcggc aggcgaaata
     1021 gcagagcgtg tggaatgaca cgtgaggcca ggcgcgtcac ggtatagcca cgatctccgc
     1081 tgatgtactg cggcacaacg ccttcctcgc cgctatccag taagatcggc tgtgcggcga
     1141 ccgcgaccgt gccgaccggg ctgagcgtgg ggatgccgcg aaactcgatc tcgtgcagat
     1201 tgcggatctc acgttccgcc atatgcggtg tcgtctcttt tatagcgtag cgtatgccct
     1261 cgcactcgac gaacaacacc gggtggcgtg actcgccacg tcggatagtc aacgccgcca
     1321 caccatgctc cggccattcg gtgagcggcg cgtgccaggg aagatgtttc aagcggtgtt
     1381 gctctgcctt gtccatatga aattgtgggc tattccgggg atgattggtc aatacgaact
     1441 tcccgttctg tgattgcgat cggctgcatt tatgagtgtt cacgatcaag atcggcaacc
     1501 acgcctgtca tcctgagcgc agcgaaggat ctcagtgtcc ggcgtgtgag atccttcgct
     1561 gcgctcagga tgaca
//
LOCUS       DASZAK010000819         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_55299, whole genome shotgun sequence.
ACCESSION   DASZAK010000819 DASZAK010000000
VERSION     DASZAK010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55299"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VGT44_14445"
     CDS             <1..585
                     /locus_tag="VGT44_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV2582052.1"
                     /translation="GQVGVWLMLIATLLTAVMTFNGGFATASRFLYAAAREDTLPSIF
                     ARISISRAVPYVAVIFLSASSAIIAIVIFVTGQFQVLILVGAVLEALIYTIAGLCVLR
                     LRRKQPDAPRAFRIWGGWTIPVLTIVVFGVLAILASLGSGSNVFLGLPLIVTIAIFIA
                     SALYVYLVIPRIRAEAQARRATVKRRRPVNPASS"
     gene            774..1193
                     /locus_tag="VGT44_14450"
     CDS             774..1193
                     /locus_tag="VGT44_14450"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2582053.1"
                     /translation="MNQNDDQDTKTANSTEVEAHLARHGGLSYLHIPALDVHQSATFY
                     ERVFGWNVHGRDTDHPGFDDGGGYVSGAWVTDQAISREPGLLPYIYVNRIDDTIERIA
                     AQGGEVVRAPYPEGNLWVATFRDPAGNVIGIWQEGPR"
     gene            complement(1200..>2085)
                     /locus_tag="VGT44_14455"
     CDS             complement(1200..>2085)
                     /locus_tag="VGT44_14455"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEV2582054.1"
                     /translation="GRPFSDPLIAHIADISALQELTSHIPTSATRLAEAFWPGPLTLI
                     LPRGQRVPSLMTAGLDTVAVRMPRHPVALALIRALGSPVAAPSANRFTHVSPTTAGHV
                     LADLGGRIPLILDSGPCEVGVESTVLNLCSAIPTILRPGGVSLEELRSVLPEAQPPEQ
                     RGPAQTDNEAQTAPGQLMTHYSPAVPLLLFDGLPVMMRAAMLAELKRRRAAGEVVGLL
                     VADEDMAAFEGSGALVRSLGDAANPAQIANNLFAALRSLEDAQVQVILCRNFNAAGLG
                     LAIRDRLLKAAGGEVIHV"
BASE COUNT          366 a          661 c          685 g          373 t
ORIGIN      
        1 gggcaggtgg gcgtgtggtt gatgctgatt gcgaccctgc tgacggcggt catgaccttc
       61 aacggcggtt tcgccacggc ctcgcgtttc ctctacgccg ccgcgcgcga agacacgctg
      121 ccgtcgatct tcgcgcgcat cagcatctcg cgcgccgttc cctacgtcgc ggtgatcttt
      181 ctgagcgcct cttccgccat catcgccatc gtgatctttg tgacggggca gttccaggtg
      241 ctgatcctgg tgggcgctgt cctcgaagcg ctgatctaca cgattgccgg cctatgcgtg
      301 ttgcgcctgc gccgcaagca gcccgacgct ccgcgcgcct tccgcatctg gggcggctgg
      361 acgatacccg tcctgacgat cgtggtgttc ggtgtgctgg cgatcctggc ctcgctcggc
      421 agcggctcga acgtcttcct cggcctgccg ctgatcgtca ccatcgccat cttcatcgcc
      481 tccgcgctct acgtctacct cgtcattcca cgcatacgcg ccgaggccca ggcgcgccgt
      541 gcaacggtga agcggcgcag gccggttaat ccggcaagtt cgtagcggag ccattctcct
      601 ctctgtagga gccggttgac cgtgctcgca gccgattgat cggcgcgaat ccttgctgat
      661 tcgcatggct ggactgaccg ataatgcaga tttacgaata aatgcggcca ccagagcaag
      721 ctccagggcg accgcatgat atactcaaga tgaacaagag aaaggagaca gcagtgaacc
      781 agaacgacga ccaggataca aagacggcca actcgacgga ggtagaggct cacctggcca
      841 ggcacggggg actctcctac ctgcatatac ccgcgctcga cgtccaccag tcggccacct
      901 tctacgaacg ggtcttcggc tggaacgtac acgggcgcga caccgaccac cccggttttg
      961 atgacggggg cggatatgtc agcggcgcgt gggtgactga ccaggcgata tcacgcgagc
     1021 ctggcctgct gccgtacatc tacgtcaacc gcatcgacga caccatcgag cggattgcgg
     1081 ctcagggtgg tgaggtcgtc agagcgccct atcccgaagg aaacctgtgg gtcgcgacgt
     1141 ttcgtgatcc cgccggcaat gtgatcggca tctggcagga aggtccccgc taaagcgatt
     1201 tacacgtgga tcacctcgcc cccggcggct ttgaggaggc ggtcacggat ggccaggccc
     1261 aggccggcag cgttgaagtt gcggcagagg atgacttgca cctgggcgtc ttcgagggaa
     1321 cgcagggcgg cgaagaggtt gttggcgatt tgcgcggggt tggcggcgtc tcccagcgaa
     1381 cgcaccagcg cgccgcttcc ctcgaaggca gccatatctt cgtcggccac cagcaggccc
     1441 actacttcgc ccgccgcccg ccgccgtttg agttctgcca gcattgccgc gcgcatcatg
     1501 acgggcaaac catcgaatag caaaagcgga accgccgggg aatagtgcgt catgagctgc
     1561 cccggcgccg tctgcgcttc gttgtcagtc tgtgctggcc cgcgctgttc gggcggctgc
     1621 gcctcaggca gcacagaccg taactcctcc aggctgacgc cgccgggtcg gaggatagta
     1681 gggatggcgc tgcacaggtt gagtacggta gactcgacgc ccacttcgca ggggccgcta
     1741 tcgaggatga gagggatgcg cccacccagg tctgccagca cgtgaccggc ggtcgtgggg
     1801 ctgacatgcg tgaagcggtt ggcgctgggc gcggcgacgg gagaaccgag cgcgcgaatg
     1861 agggcgagcg cgaccgggtg gcgcggcatc cgcacggcga cggtgtccag cccggcggtc
     1921 atcaacgatg gcacgcgctg gccgcgcggc aggatcagcg tcagcggccc cggccagaag
     1981 gcttcggcca gccgcgtcgc agaggttgga atatgcgacg ttaactcctg caaagcgctt
     2041 atgtcggcga tgtgcgcaat cagggggtcg ctaaagggac gccct
//
LOCUS       DASZAK010000820         4886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_233535, whole genome shotgun sequence.
ACCESSION   DASZAK010000820 DASZAK010000000
VERSION     DASZAK010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233535"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1642)
                     /locus_tag="VGT44_14460"
     CDS             complement(113..1642)
                     /locus_tag="VGT44_14460"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEV2582055.1"
                     /translation="MPTSQEAPLSTLEEASISLLQAAMTGGQVTSQQLVERYLERIEA
                     IDRSGPAINAVIEINPDCLSIAGERDEERASRGVRGPLHGIPILIKDNIATADAMQTT
                     AGSLALVGSRVPRDAFVVQQLRRSGAIILGKANLSEWANFRSSPSSSGWSGRGGQTRN
                     PYVLDRTPCGSSSGSGAAIAASLAAAALGTETDGSILDPSAINGLVGIKPTVGLTSRD
                     GVIPIAHSQDTVGPMARSVADAAALLGAITGIDERDPATHASAGRFHKDYTKFLERDG
                     LRGARIGIARQAYFGYSPKADAIIEAAIEHMRALGAEIIDNANIPTTKQMAESNTEMT
                     VLLYECKADLNAYLAGLLESPVRTLAEVIDFNLAHADQELQFFGQELFLKAQDTAGLH
                     DPAYLEALETNHRLSREQGIDAVMDEYRLDALVAPTTGPAWRIDLVNGDHHMGASTQP
                     TALSGYPAITVPAGYAFELPIGITFMGRAYSEPTLIRLAYAFEQATKARRTPKYLPTT
                     P"
     gene            1835..3340
                     /locus_tag="VGT44_14465"
     CDS             1835..3340
                     /locus_tag="VGT44_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV2582056.1"
                     /translation="MATIFETPNATDSALPPGPRPLPFIGNVLAFRRDQLGFLQELER
                     TYGKMATIYIGTTPVVLLCRPEHVRYVLTENPRNFTNREMAEGLRQLIGDGLLTIDGE
                     AHRQQRRLVQPAFHRKRIEGYAATMTQYAQEMLADWRGGDRLDVARAMQELTLRIVAK
                     CLFNVDLADQVNELGDAFSGMIDNPVGLLEGFLNIRVDSQLTAYGRRMAARRRVDEFI
                     YALIAQRRADDGQDKGDVLSMLLAAQESGEGEEHLEALSDTQVRDHIMTFVAAGHETT
                     ANALTWTFYLLSRHAEARERLLAELESVLAGRTPTLEDLPQLPYLEWVLNESMRLYPP
                     AWVQGRRSIEAFDLDGVHFPAGTMVLFSQWVIQRLPGIWGDAEAFRPERWDPASDAKT
                     PPWSYFPFGGGPRICIGMPFAQLEAKLLLATILQRFTPRVDPGYRLELNPLITLRPKH
                     RMPVVLLPTGAADAAEGAPAWEGLLYGNDERQQEGYAERKGCLGAFMSLFL"
     gene            complement(3421..4194)
                     /locus_tag="VGT44_14470"
     CDS             complement(3421..4194)
                     /locus_tag="VGT44_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582057.1"
                     /translation="MKKVAISGTLLVVALLSLVWFVPTLAARTQSQHTTHQQTIFALE
                     HQVGKPGGAAIGAAITKLPYTCTGAVKGNMLINVTEKVVNDGDSGQAGNYWAYDTFSR
                     TIQVWNVGSDSYCALVNYAGTFAAVAGQKSPGTVGSNGGILTGDEVGTIKGGYHMLIT
                     ASLDVSAPATWPLVGKVNGGNAVDYQCVIAVNGSENCPGFVDWTAQYFSTGGSYNEDL
                     WGWTYVGKDAPTAPDAGTADGVWHNFYSGNSGDILDVDP"
     gene            4585..>4886
                     /locus_tag="VGT44_14475"
     CDS             4585..>4886
                     /locus_tag="VGT44_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582058.1"
                     /translation="MSFESLLPVVGEMAVLLIVATALLWPAPVDSNQLFAAQLSSDLL
                     ISHWPTALLIQGTFAQLHTLPLWNPYFGGGQPLGADPLAALFYPPTHLLHFLSLRD"
BASE COUNT          945 a         1358 c         1523 g         1060 t
ORIGIN      
        1 ccgcgtaccc gctggagcaa aggaatcgtc ggaacaacag gtacgtgggc gacgcaagcg
       61 tcccctcccc tcgtccgcac cacccccgcc cctacggaac tgtgggcagg ttttatgggg
      121 tggtgggcag gtatttgggc gtccttcttg cttttgtggc ctgttcaaag gcgtaggcga
      181 ggcggatgag ggtaggttcg ctgtaggcgc ggcccatgaa ggtgatgccg atgggcagct
      241 cgaaggcgta gccggcaggg acggtgatgg ctgggtagcc ggagagggcg gttggctgag
      301 tgctggcgcc catgtggtga tcgccgttga cgaggtcgat gcgccaggcc gggccggtgg
      361 tgggcgcgac cagcgcgtcg aggcggtatt cgtccatcac ggcgtcgatg ccttgctcgc
      421 gagagagtcg gtggttggtc tcaagcgcct ccaggtaggc gggatcatgc aacccggcgg
      481 tgtcctgtgc cttcaagaac aactcctgcc cgaaaaactg gagttcctgg tcggcgtggg
      541 cgagattgaa gtcgatcacc tcggccaggg tgcgcacagg cgattcgagt agtccagcga
      601 gataggcgtt caggtcggcc ttacattcgt agagcagcac ggtcatttcc gtattagact
      661 cggccatctg tttggtggtc gggatgttgg cgttgtcgat gatctcagcc cccaacgcgc
      721 gcatgtgttc gatagcggcc tcgatgatag cgtcggcctt cgggctgtag ccgaaatagg
      781 cctgacgcgc gatgccgatg cgcgcgccac gcaatccatc cctctccaga aatttcgtat
      841 agtctttatg aaatcgcccc gcgctggcat gggtggccgg gtcgcgctcg tcgatgccgg
      901 tgatcgcgcc cagcagggcg gcggcatcgg ccacactgcg cgccattggc ccgacggtgt
      961 cctggctgtg cgcgatgggg atgacgccat cgcggctggt gaggccgacg gtgggcttga
     1021 tgccaaccag cccgttgatg gccgatgggt cgaggatcga gccgtcggtt tccgtgccaa
     1081 gcgcggctgc cgcgaggctg gcggcgatgg ctgcgccgga accagaactg gagccgcagg
     1141 gggtgcgatc cagtacatag gggttgcgcg tctgcccgcc gcgcccgctc cagccgctcg
     1201 aagaagggct agagcggaaa ttagcccatt cgctcaggtt ggctttgccc aggatgattg
     1261 ctccggagcg gcgcagttgc tgcacgacaa aggcgtcacg cggtacgcgc gagccgacca
     1321 gcgccagcga tcccgccgtc gtctgcatgg cgtcggcggt tgcgatattg tctttgatga
     1381 ggatggggat gccgtgcaac ggcccgcgaa cgccgcgcga ggcgcgctcc tcgtcacgct
     1441 ccccggcgat agagaggcag tcagggttta tctcgataac cgcattgatg gcaggcccag
     1501 agcggtcgat ggcttcgatg cgttcaagat agcgctcgac aagctgttgc gacgtgactt
     1561 gcccgcctgt catggctgct tgtaagaggc tgatactggc ttcttcgagg gtagacagtg
     1621 gcgcttcctg ggatgtgggc atggcgggat ggctcccttc tcctcttatg gatagtatga
     1681 aacctgtagg agccggttca ccgcgctcct acggtttttt catggacgcg tgtccggtgt
     1741 gtatcaatgt cgtgcgtccg atgcgcatat atgatatggt ataacataga gtcaaatggc
     1801 taagttaccg gatatatacg agagaagcat ctttatggca accatatttg aaacacccaa
     1861 tgcgactgat agcgcgttgc cgcctggccc caggccgctg ccgtttattg gcaatgtgct
     1921 ggcattccgc cgcgaccagc tggggttttt gcaggagctt gaacgcacgt atggcaagat
     1981 ggccacgatt tatattggga caacgccggt ggtactcctt tgcaggccgg agcatgtgcg
     2041 ctacgttttg acggagaatc cgcgcaactt tacgaatcgt gagatggccg aggggttgcg
     2101 ccagttgatc ggcgatgggc tgctgacgat cgacggggag gcgcatcgcc agcagcgccg
     2161 cctggtgcag cctgccttcc ataggaagcg catcgagggg tacgcagcga cgatgacgca
     2221 gtacgcgcag gagatgctgg ccgattggcg cggcggagac cgcctggacg tggcgcgcgc
     2281 catgcaggag ctgacgctgc gcattgtggc gaaatgcctc ttcaacgtgg acctggccga
     2341 ccaggtgaac gagctgggcg acgctttttc cgggatgatc gataacccgg ttggcctgct
     2401 ggaaggtttt ttgaatatcc gcgtcgattc gcaactgact gcttatggac gacgtatggc
     2461 ggccaggcgc cgggtggacg agttcatcta tgccctgatc gcgcagcggc gcgccgacga
     2521 tgggcaggat aagggcgatg tgctgtcgat gctgctggcg gcgcaggaga gcggagaggg
     2581 cgaggagcat cttgaggcgc tgagcgatac acaggtgcgc gaccacatca tgaccttcgt
     2641 ggccgccgga cacgagacga ccgctaacgc tctgacatgg acgttctacc tgctgtcgag
     2701 acatgccgag gcgcgtgaaa ggttgctcgc ggagctagag agtgtgctgg cgggacgcac
     2761 accgacgctt gaagatttgc cacagttgcc ttacctggaa tgggtgctga acgagtcgat
     2821 gcggctctac ccgcctgcct gggtacaggg ccgccgctca atagaggcat tcgatctgga
     2881 tggcgttcat ttcccggcag ggacaatggt cttattcagc cagtgggtca tccagcgcct
     2941 gcctggcatc tggggcgatg ctgaagcctt ccgccctgaa cgctgggacc ctgcttccga
     3001 cgcgaaaacg ccgccctggt cgtatttccc ctttggcgga gggccgcgca tctgcatcgg
     3061 gatgcccttt gcgcagcttg aagcgaagct gctgctggcg accatcctgc aacgcttcac
     3121 gccgcgcgtc gatcctggct atcgtctcga actgaacccg ctcatcacgc tgcgcccgaa
     3181 gcataggatg ccggtcgtcc tgcttccgac cggcgcagcg gacgcggcgg agggcgcgcc
     3241 cgcgtgggag ggtctgctct acggcaacga tgaacggcag caagaagggt acgccgaacg
     3301 caagggatgc ctgggcgcgt ttatgtcgct gtttctctaa ctgagtttgc gaagttttac
     3361 aaagaaaagg agcggagcca caaacgactg gctccgctcc ctcttaatac gacatggcta
     3421 ttacggatca acatccagga tatcgccgga gttgccggaa tagaagttgt gccacacgcc
     3481 atcagccgtg cctgcatcgg gggccgtggg cgcatccttc ccaacgtagg tccatcccca
     3541 taaatcttcg ttgtacgagc cgcccgtgct gaagtactgc gcggtccagt caacgaatcc
     3601 cgggcagttt tcgctgccat tcaccgcaat tacgcactga taatcaaccg cgttgccacc
     3661 gttgactttg ccgaccagcg gccaggtcgc tggcgcgctg acatcgagcg aggcggtgat
     3721 cagcatatga taaccgcctt tgatcgtgcc gacttcgtcg cctgtcaaga taccaccatt
     3781 gcttccaacc gtgccagggc ttttttgccc ggcgacggcg gcgaatgtgc cggcataatt
     3841 gacaagcgcg cagtagctgt cagaccctac gttccaaacc tggatggtgc gggaaaacgt
     3901 atcgtacgcc cagtagttgc ccgcctgccc cgaatcacca tcgttcacga ccttttccgt
     3961 cacgttgatc aacatgttgc ccttgaccgc gccggtacag gtatagggca acttggttat
     4021 cgccgcgcct atcgctgccc caccaggctt tccgacctgg tgttctaagg caaaaatggt
     4081 ttgttgatgc gtagtgtgct gtgactgcgt gcgcgccgcc aatgtgggaa caaaccatac
     4141 cagggacagc aatgcaacga cgagcagagt cccgctaatg gctacctttt tcataaaact
     4201 ccctcccttt gttcagcaaa gaagcgacta acgaatctac aaatattgtc aagttacttt
     4261 gctatcccgg tcgaacagca attggctata ctctagcaaa caaccatgtc gatatggttg
     4321 ctgtttggtt acgatcaggt tgtatctata taaattctgt agtgttctat caacttccaa
     4381 ttttcaatta aattgctact attacgctca aatggcttga gaacaagcag gaatacaaca
     4441 ttcgcttaca ggcagatggg agtggaggtg tgattttgcc aggaaaagat acgtcatgta
     4501 gtatgatatc ctgaaatcac agaaggtagg gtcgaataga atttgaagaa cagcaagctc
     4561 gaggtaaaac gacgcttttg gggagtgagt tttgaatctc tgctccctgt agtgggcgag
     4621 atggccgttc tcttgattgt cgcaaccgcg ctgctctggc ccgcgcctgt tgactcgaac
     4681 cagttgttcg ctgctcaact gagtagtgat ttgttgatta gccactggcc tactgctctt
     4741 ttgatccagg gcacattcgc gcaattgcac accttaccat tgtggaatcc gtacttcggc
     4801 ggcggtcagc cgttgggggc cgatccactt gccgcgctct tttacccacc gacgcacctg
     4861 ctacatttcc tctctttgcg cgatta
//
LOCUS       DASZAK010000821         5974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_304272, whole genome shotgun sequence.
ACCESSION   DASZAK010000821 DASZAK010000000
VERSION     DASZAK010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5974
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304272"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1071)
                     /locus_tag="VGT44_14480"
     CDS             complement(<1..1071)
                     /locus_tag="VGT44_14480"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV2582059.1"
                     /translation="MRVAINAQLLSFSDNYRNGGISRYIRSLLTELARQAAEQSEQPG
                     QVGNHDYTIFVNGREVVERLQRELPASPSVTYVAAAWPESRPVSRVTWEQFTLPGLLR
                     KRRIDVFHSPANVLPELLPRGCKGVVTLHDLAFLRYPHVLTRAKRIYHRAFTTRSLRR
                     ATLIIANSDSTKRDAIELVGIAPERIQTVYPCIDTRFSHVITNEEIASFRQQSGLEGG
                     YILYLGTLEPRKNIPALLEAYARLRSEYQRTEKLALVGGKGWLYDEIFARAQALGLAS
                     DVLFTGFVSDADQLLWYRAASAFVYPSLYEGFGLPVSEALACGIPVVTSSVSSLPEAG
                     AGLALTVNPGDIEGLVGAMLQAL"
     gene            1313..3682
                     /locus_tag="VGT44_14485"
     CDS             1313..3682
                     /locus_tag="VGT44_14485"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV2582060.1"
                     /translation="MSNQLRLDVAQLTDVGRKREHNEDNMAYVIPKDEQVMATKGALF
                     IVADGMGGHAAGEVASEIAVDTVSNTYYMDDNIDVAAPLLQAIKRANAAIHQRAAENM
                     LRSGMGTTCVAAVLRGNVAYIANVGDSRAYLVRQGQVRQISLDHSWVAEQVRANLLTE
                     EQARTHAQRNVITRSLGTQPDVDVDLFREQLQPGDYLVLCSDGLSGLISDEELKRTVE
                     QSVPQESVYHLVERANENGGPDNITAIVVQVQEVGVEPPGVRNPVLVGGREVSDEETI
                     AMNMISGPARNAVSSGHYISGPLRLSSGPLASPDSVTAPQPALSRMRRSRLFFPTLLL
                     IFLLIVALAGGGVFYVLHLNQSQAISQSLTNANQSITQAAGLTSGSNPNPNPAQALMD
                     LSSAQKTLQNIQQNQLDSSQSSNFKSLQNQLVIQTNQAIKAYNSQASIFPLSCYGYST
                     SVLNLGNNNTQAASVAAVQNGRSTVLFALGSDGALYRLNGPSVVPSSTSSKILAIASD
                     GKQLFLLNEQINNSVPAYSVSVYSFNPSGSLGQPQSIKISQSLITGGFTPLPFISAWG
                     NHVYVAFNEPVASNGSDVAHIVSYAPDKTGQLAASQPFTISVSHPISSVAAFPTQFFL
                     LLADGEVLSFPLDAAFGTQTNKPPPAPVQVQAPIQTPLPVAGQFTQATSVPTPTATQP
                     ATSGIVTLTVPNAAQLAVGVVSNTTHLYIGDSSNHRVIDLVVASSTGTSVTMKLIQQY
                     VSAQLLNKVESIAVDPGGATMYLLDQVSAKQISLISVSTALQSGCGTGI"
     gene            4039..5826
                     /gene="aspS"
                     /locus_tag="VGT44_14490"
     CDS             4039..5826
                     /gene="aspS"
                     /locus_tag="VGT44_14490"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV2582061.1"
                     /translation="MDFAVHFRTHTCGELNAQAIGQQVTLAGWVNRRRDHGGLVFLDI
                     RDRYGITQVICDPERSPATHSRASELRSEFVVQVKGSVVARLPGTENPNLSTGAIEVA
                     ADEIEILNPSRTTPFPISDTISVDESLRLKYRYLDLRRPSMRDNIILRHRVVKAIRDY
                     LDARGFLEIETPILMKSTPEGARDYLVPSRIYPGYFYALPQSPQQLKQLLMVAGLDRY
                     FQIARCFRDEDQRSDRQPEFTQLDLEMSFVSEDDVMSLIESLLIELIGQTTGKRIKQQ
                     PFPRLSYEHVMDRYGTDHPDLRFDLPLVNISDLASAGSFGVFQGALATNGMVKGIRVP
                     GAGSYSRKEIEELTEFARMLGAKGLVSLAIGPTGEIKSPLTKFMASGEVQAIVDRLQG
                     EQGDLLLFVADSAKVCNDVLFRLRVRLAERLKLIDPGEMALCWVVDFPLLHYDEAEQR
                     YEAEHNPFSGMDEAHVDRLDSDPLHIRAKQYDIICNGYEIGGGSVRINVAALQHKVFS
                     LMKLNDEQIREQFGHMLESFEYGAPPHGGIALGIDRLVMLFADEDSIREVIVFPKSQA
                     AQDLLMNAPSLVDEKQLQELHLRLREEGR"
BASE COUNT         1323 a         1693 c         1698 g         1260 t
ORIGIN      
        1 caatgcctgg agcatggccc caaccaaccc ttcgatatct ccgggattaa ccgttagcgc
       61 caatcctgct cccgcctccg gcaggctgga gacgctggat gtcacaaccg ggataccgca
      121 ggccagcgcc tcgcttacag gcagcccgaa gccctcgtaa agcgatggat agacgaacgc
      181 cgacgcggcg cgataccaca ggagctggtc cgcgtcggaa acaaacccgg tgaagagtac
      241 gtctgacgcc agccccaggg cctgcgcgcg cgcaaaaatc tcatcgtaca gccagccctt
      301 tccgccgacc agcgcgagtt tctctgtgcg ctgatattct gaacgcaagc gggcataggc
      361 ttcgagcagc gccgggatgt tttttcgcgg ctccagcgtg ccgaggtaca ggatatagcc
      421 gccctccaac cctgattgct ggcgaaatga cgcaatctcc tcatttgtaa taacgtgaga
      481 gaagcgcgta tcgatacatg ggtacaccgt ttggatgcgc tcaggcgcga tgccaactag
      541 ctcgatggca tcccgtttcg tgctatccga attggctatg atgagcgtgg cgcgcctcag
      601 gctgcgcgtg gtgaaagcgc ggtgatagat gcgctttgcc ctggtgagta catgcgggta
      661 gcgtaaaaag gccaggtcgt gcagcgtaac gactcctttg cacccgcgcg gcagcaactc
      721 cggcagaaca tttgcaggtg aatggaacac gtcgatgcgc cgtttgcgca gcagacctgg
      781 caaggtgaac tgctcccagg ttacacgcga aactggcctg gactcaggcc aggccgctgc
      841 gacatatgtg acagatggtg atgccggtag ctcgcgctgg agccgctcta ctacctcccg
      901 cccattcaca aagatggtgt agtcgtggtt gccgacctgc cccggctgtt ctgattgttc
      961 agcagcctgg cgcgccagtt cggtcagcaa agagcgaatg tagcgactga ttccgccatt
     1021 ccggtagttg tcactgaatg agagtaattg agcattgatc gcgacgcgca tatacatctt
     1081 ttctgtagag catagctatt ggtagagacc tcactgctgt ataacgtccc tgtgtgaaca
     1141 caggtaacag ccttagaaat actggccggt ggtacttcca tgagccaaat cgtgtgagcc
     1201 attgcgtttt tcattgcatt ggagtattat agccacaagt tcaaagggac agatagcaat
     1261 ggggcagggt tgtcgcaaag taagtagccc agtgattacg ggagaaggtg cgttgtcgaa
     1321 tcagcttcgc ctcgatgttg cgcaactgac cgacgttggc cggaagcgcg aacacaacga
     1381 ggataatatg gcctatgtta tcccaaagga tgagcaggtc atggcgacga agggggcgct
     1441 gtttatcgtt gccgatggca tgggggggca tgcggctggt gaagtggcga gtgagatcgc
     1501 ggttgatacg gtaagcaaca cctattatat ggatgacaat attgatgtcg ctgcgccgtt
     1561 gttgcaggcc atcaagcgcg cgaacgcggc cattcaccag cgcgccgccg agaacatgct
     1621 gcgcagcggc atgggaacca cctgtgttgc cgccgtcctg cgtgggaacg tggcgtatat
     1681 tgccaatgtc ggtgatagcc gcgcctatct tgtgcgccag ggccaggtcc ggcagatttc
     1741 cctggaccac tcctgggtgg ccgagcaggt gcgcgccaac ctgttgaccg aggagcaggc
     1801 gcgcacacac gcccagcgca acgtcatcac gcgcagcctg ggaacgcagc ccgatgtcga
     1861 tgtagattta ttccgcgaac aacttcagcc gggcgattac ctggtgcttt gctctgatgg
     1921 actctccggc ttaattagcg atgaagaatt gaagcgcacc gttgagcaat ccgtgccgca
     1981 ggaaagcgtc tatcacctgg tggagcgcgc caacgaaaat ggcggccccg acaatatcac
     2041 cgcgatcgtg gtgcaggtgc aggaagtcgg cgtggagcct cccggtgtgc gcaatcccgt
     2101 gctggtaggc gggcgagagg tgagcgacga agaaaccatc gccatgaata tgatttccgg
     2161 cccggccaga aatgccgtca gcagtggaca ctatatcagc ggcccattgc gcctgagcag
     2221 cggcccgctt gcctctcccg acagcgtgac agcaccgcag cccgcgttga gcaggatgcg
     2281 ccgcagccgc ctcttttttc cgacgctgct gctgatcttc ctgctcatcg tggccctggc
     2341 gggcggcgga gtattttatg tcttgcatct caaccagagt caggcaatct cacagtccct
     2401 gacgaacgcc aatcagtcga ttacccaggc agcaggcctg acgtctggca gtaatccgaa
     2461 tccgaatccg gcgcaggcgc ttatggattt gtcctccgct caaaagacct tgcagaacat
     2521 acagcaaaat cagcttgata gcagccagtc gagcaatttc aaatcgctac aaaatcagct
     2581 cgtcatccag acgaatcagg ctatcaaggc atataattcc caggcgagta tctttccgct
     2641 ttcttgctat ggctatagta catcggtgtt gaatttgggc aacaacaata ctcaggctgc
     2701 tagcgtcgct gcagtacaga acgggagaag cacggtgtta tttgcgctcg gcagcgatgg
     2761 agcgctctat cgactcaatg ggccgagcgt tgttccttcg tcgacgtcca gcaagattct
     2821 agctatcgcg agcgatggca aacagctctt cttgctaaac gagcagatca acaatagtgt
     2881 tcctgcttac agtgtgagcg tgtattcgtt caatccatcg ggatcgctgg gacaaccaca
     2941 gagcatcaag atcagtcaga gcttgattac cggcggattt acgcctttac cgttcatctc
     3001 ggcctggggc aatcatgtct atgtagcgtt taacgaaccg gttgcgtcta acggatcgga
     3061 cgtagcgcat atcgtctcat acgctcctga taaaaccgga cagctggcgg catctcaacc
     3121 cttcacgatt tccgtttcgc acccaatctc cagcgtagct gccttcccga cgcagttttt
     3181 cttgttactg gcggatggtg aggttctgag cttccccctt gacgcggctt ttggcacaca
     3241 gacgaacaaa ccgcctcctg ctcctgtcca ggtacaggcg ccaatccaga cgccgctgcc
     3301 ggttgccggt caattcaccc aggcgacttc agtgccaacg ccaactgcga cgcaaccggc
     3361 gacatctgga attgtgacgc taaccgtgcc gaacgcggca cagctcgcgg taggggttgt
     3421 gagtaacacc acacatttgt acatcggcga ctcaagcaac catcgcgtga ttgacctggt
     3481 agtggcgtca tcaaccggga catcagtcac gatgaaactt attcaacaat atgtctccgc
     3541 tcaactacta aataaagtgg agagtatcgc cgttgatcct ggtggtgcta cgatgtatct
     3601 tctcgatcaa gttagcgcaa aacagatcag cctgatctcg gttagcaccg cgctgcaaag
     3661 tggttgtgga acaggaatat aatgataccg ttgatgaaat caagagtagt gacgaaaggg
     3721 cgaccacaag ggcttacagg agtgggtttt taggaaagga accaaccact tcgactgtca
     3781 tcctgagcgc agcgaaggat ctcggtctgg cccgcgagag agagactgtg tgaaaagcgc
     3841 tcaggtgcag tcaaaatgag agaaaagaag tggctcgaca ggccgaagga aacttctttt
     3901 cacacagtct cagatccttc gctgcgctca ggatgacagc ctctggcgac cgcgtgtttc
     3961 aaaaaaccta cccttgtggt cgccctttgg gaatttgcca acagtatctt ctaaaatgca
     4021 cataaggagc ttggatacgt ggattttgct gtacatttcc gcacacacac ctgtggtgaa
     4081 ttaaatgcgc aagctattgg ccagcaggtg acgctggccg ggtgggttaa tcgccgtcgc
     4141 gatcatggcg ggctggtttt tctcgatatc cgtgacaggt atggcattac ccaggtcatc
     4201 tgcgatcccg aacgctcgcc tgccacgcac agtcgcgcat cagaactgcg ttcggagttt
     4261 gttgtccagg tcaaaggtag cgtcgttgcg cgcctgcctg ggaccgagaa tccgaacttg
     4321 agtaccggcg cgatcgaggt cgctgccgat gagatagaga tactcaatcc ttcgcgcacc
     4381 acgccgtttc ccatcagcga caccatcagc gttgacgagt cgctgcgtct caagtatcgc
     4441 taccttgacc tgcgccgtcc gtctatgcgc gataacatca tcctgcgcca ccgtgtcgtc
     4501 aaggccattc gcgactacct ggatgcgcgc ggcttcctgg agatcgagac gcccattttg
     4561 atgaaaagca caccggaggg tgcgcgcgac tacctggttc ccagccgcat ctatccaggc
     4621 tacttctacg ccctgccgca gtcgccccaa cagctcaagc agttgctgat ggtggccggg
     4681 ctggatcgct atttccagat cgcgcgctgt ttccgcgatg aggaccagcg ttccgaccgc
     4741 cagccggaat ttacgcagct cgacctggaa atgtcctttg tctccgaaga tgatgtcatg
     4801 tcgctgatcg agagcctctt gatcgagctg atcggacaaa cgaccggcaa gcgcatcaaa
     4861 cagcagccct tcccgcgcct gagctacgag catgtcatgg atcgctatgg caccgaccat
     4921 cccgacctgc gcttcgacct gcccctggtc aatatctctg acctggcctc ggctggcagt
     4981 ttcggcgtgt ttcagggcgc gctggcgaca aatggcatgg ttaagggcat tcgcgtgccg
     5041 ggcgcgggca gctacagccg caaagagatc gaggaattga ccgagttcgc gcgaatgctc
     5101 ggcgcgaagg gacttgtttc cctggccatc ggccctaccg gcgagatcaa atcgcctttg
     5161 acgaaattca tggcgagcgg cgaagtgcaa gccatcgttg atcgcttgca gggcgagcaa
     5221 ggcgatctgc tgctctttgt ggccgatagc gcgaaggtct gcaatgatgt gctattccgc
     5281 ctgcgcgtcc ggctggccga gcggctcaag ctgatcgacc ccggcgaaat ggcgctctgc
     5341 tgggtggttg atttccccct cctgcattac gatgaagctg agcagcgcta cgaggccgaa
     5401 cacaacccct tctccggcat ggacgaagcc cacgtagacc ggctggatag cgatccgctg
     5461 catatccgcg cgaagcagta cgacatcatc tgcaatggct acgagatcgg cggcggcagc
     5521 gtgcgtatca acgtcgccgc cttgcagcat aaggtcttct cgctgatgaa gctgaatgat
     5581 gagcagattc gagagcagtt cggccacatg ctcgaatcgt ttgaatatgg cgcgccaccg
     5641 catggcggca tcgccctggg catcgatcgc ctcgtcatgc tcttcgccga cgaggacagc
     5701 attcgcgaag tgatcgtctt ccccaaatca caggccgcgc aagacctgct gatgaacgcc
     5761 ccttcgctcg tagacgaaaa gcagttgcag gaacttcact tgcgactgcg tgaagaaggt
     5821 agatagcggc gctatcaccc agccactcga ccaactcgtg atagagttgc tcgaggcggc
     5881 gagtggacga gcgggataaa agcggatacg gagggcgacc acaaggcgta caccgaagtt
     5941 gctccctcct gtcatgctga gcgcagcgaa gcat
//
LOCUS       DASZAK010000822         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_55321, whole genome shotgun sequence.
ACCESSION   DASZAK010000822 DASZAK010000000
VERSION     DASZAK010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55321"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VGT44_14495"
     CDS             complement(<1..564)
                     /locus_tag="VGT44_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582062.1"
                     /translation="MGASAMSRRTTLFFATDIHGSERCFIKFINAAKFYQANVLILGG
                     DITGKAMVPIVRQSGGASYRATLLGQTVSLESEEAIAAFERRVRQAGAYPFRTTTEEV
                     AAMEADRSLVDRLFTRLMVESVEGWCEIAEERLRGTGVRCFIQAGNDDEREIVEVLKQ
                     CSYVEMPEGRVVVIDDEHEMVSSGFANT"
     gene            complement(574..1359)
                     /locus_tag="VGT44_14500"
     CDS             complement(574..1359)
                     /locus_tag="VGT44_14500"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2582063.1"
                     /translation="MLLAGLHGFITGAGAGIGRAIALEMVREGVDLYITDLDAEAAQR
                     VAGEANALRPEARIWAAQLDVRNAADVTTRVEDAAQRLGGLELAVNNAGVSSMARVVD
                     LTEADWDFNMDVNAKGIFFCCQAEVRLFLRRGGGGTIVNTASMAGKKAAPLLAHYSAS
                     KFAVIGFTQALALETAAYGIRVNSVCPGYVRTSMQEREVVWESALRGITAEEVRQEYI
                     RSIPLGRLEEPEDVACVVVFLLSDRAAYMTGQAVNVTGGAIMF"
     gene            complement(1384..>2634)
                     /locus_tag="VGT44_14505"
     CDS             complement(1384..>2634)
                     /locus_tag="VGT44_14505"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEV2582064.1"
                     /translation="MVPAMVLLDTNDQPLRPSIQQNDARSIAEVTELRAAVDAEEFFT
                     ITGGIPNQQNIDPRWRWLTRHEPDIVAHVAHLCGSYDFIVFRLTGQHSLEENWAAESG
                     LYDVQQHRWHVPYLEHASIDPAILPPVHQPTEIVGSISETVAMQSGLRTGTPVVAGSA
                     DHIAAALAAGLTKNGDVLLKFGGAGDLLYCADYPEPDRHFYFDYHDIPDLTILSGCMA
                     TSGSLVKWFAQELAGGASLALLDHEAEAVPPGSSGIIALPYFLGEKTPIFDPAARGVF
                     AGVMLHHTRAHLYRSILEAVCYGFAHHISLLREAKRPIRRVCAADGGSRSRLWMQIMS
                     DVLNQQVQVVAGEAASALGAAYVAAMGVGMFANWHEITRFISQGPIYQPQSTHAERYR
                     EGFSLYRDLYTRLQSFLPELGRLD"
BASE COUNT          604 a          803 c          671 g          556 t
ORIGIN      
        1 tgtgttggca aagcctgagc tgaccatctc gtgttcatca tcgatcacca caacgcgccc
       61 ctctggcatc tcgacataag aacattgctt gagtacctcg acgatctcac gttcgtcatc
      121 attgccagcc tggataaaac accgtacacc ggttccgcgc agccgctcct cagcaatttc
      181 acaccaaccc tctacgcttt ccaccattaa gcgggtgaac agccgatcaa cgaggctgcg
      241 gtcagcctcc atcgccgcta cttcctcggt tgttgtgcgg aatggatatg ctccggcttg
      301 acgaacccgg cgttcaaaag cggcaatagc ctcctctgat tcgagcgaga ctgtctgacc
      361 gagcaaagtc gcccggtaac tggctccccc agattggcgc acaattggca ccatggcctt
      421 gcctgtgatg tcaccaccaa gaatcagcac attggcctga taaaacttcg ccgcattaat
      481 aaatttgatg aaacagcgct cggacccatg aatatccgtg gcgaaaaaga gagtagttcg
      541 ccgactcatc gcagatgccc ccattgctta accctagaac atgatcgccc caccggttac
      601 gttcacggcc tggcccgtca tatatgcagc gcggtcgcta agcagaaaca caacaacaca
      661 tgcaacgtcc tctggttctt ccaatctccc cagtgggatc gaccggatgt actcctgtcg
      721 cacttcctcc gctgttatgc cgcgaagcgc cgattcccag accacctcgc gctcctgcat
      781 agaggttcgc acatagccag ggcacacaga attgacacgt ataccatatg ctgctgtttc
      841 gagcgcaagc gcttgcgtga agccgatcac tgcgaacttg gacgccgagt aatgggcgag
      901 gagtggagca gcttttttgc cagccatcga ggccgtgttc acgatggtac cgcctccgcc
      961 ccggcgcagg aagaggcgca cctcagcctg gcagcagaag aagataccct tcgcattcac
     1021 atccatattg aagtcccagt cggcctcggt cagatcgacc acccgcgcca tcgaggagac
     1081 acccgcattg ttgacagcga gttcgaggcc acccaatcgc tgcgccgcgt cctcgactcg
     1141 tgtcgtcacg tcagcagcat tgcgtacatc aagctgggca gcccagatac gcgcttctgg
     1201 acgaagtgca ttcgcctcgc ctgcgactcg ctgtgctgct tcggcgtcaa gatcagtaat
     1261 gtagagatcg accccttcgc gcaccatctc gagcgcaatt gcccggccaa tcccggctcc
     1321 tgcccccgtg ataaacccgt gtaaacctgc taacagcatt ctgaacatcc tttcctaaat
     1381 gaatcaatca agccggccaa gttcaggcaa gaaggattgt aatcgtgtat agagatcgcg
     1441 ataaagcgaa aagccttcac gatagcgttc agcatgcgta ctttgtggtt ggtagattgg
     1501 cccctgactg atgaaacggg tgatctcatg ccagttcgca aacatgccca cacccattgc
     1561 ggcgacatag gctgcgccca gcgccgaggc cgcctcgccg gcaactacct gcacttgctg
     1621 gtttagcaca tcggacataa tctgcatcca gagccggctg cggcttccac catctgctgc
     1681 gcacacacgc cggatgggtc gctttgcctc gcgcagtaat gaaatatggt gcgcaaatcc
     1741 atagcagacc gcttccagga ttgatcgata gaggtgggct cgcgtatgat gcagcattac
     1801 accggcgaag acaccgcgag cggctggatc aaagataggc gtcttctcac ccagaaaata
     1861 aggcagcgca atgatcccac tagagccggg cggaaccgcc tcagcctcgt gatccagcag
     1921 cgcgagggat gctccgccag ccagttcttg cgcaaaccat ttcactaacg agccgctggt
     1981 agccatgcat ccactgagta tcgtgaggtc agggatatcg tggtaatcga aataaaaatg
     2041 ccgatcgggt tcaggatagt ctgcacaata gaggagatcg cccgcgcccc caaacttcag
     2101 cagaacatcg ccgttcttcg tcagtccggc ggctagtgct gccgcgatat gatcggcgct
     2161 cccagctacc actggcgtcc ctgttcgtag gccactctgc atggcaaccg tctcgctaat
     2221 acttcccacg atctcggtcg gttgatggac tggtggtaag attgcaggat caatgctggc
     2281 atgctccaga tagggcacat gccagcgatg ttgctggacg tcgtagagcc cactttctgc
     2341 tgcccagttc tcctctagag agtgttggcc agtcagtcga aacacaatga aatcatagga
     2401 gccacacaaa tgcgccacgt gcgccacaat gtctggttca tgtcgtgtca gccagcgcca
     2461 gcgaggatca atattctgct gattgggtat gcctccggtg atggtgaaaa attcctccgc
     2521 atcaacggca gcacgcaatt ctgtgacctc ggcgattgaa cgtgcgtcat tctgctggat
     2581 actcggtcgc agtggctgat cgtttgtgtc cagcaagacc atcgcaggca ccat
//
LOCUS       DASZAK010000823         1598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_55407, whole genome shotgun sequence.
ACCESSION   DASZAK010000823 DASZAK010000000
VERSION     DASZAK010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55407"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VGT44_14510"
     CDS             complement(<1..1246)
                     /locus_tag="VGT44_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2582065.1"
                     /translation="MAEQSPHEPRAREEGRPDPEELLRRYSLRDSDLEAPPASPVPGS
                     DALVVPQPAAAGYTRRRGRLRVYLASAAGSGKTYAMLNEGHRREGRGANVVIGYVETH
                     ARSQTEAQIGDLKIIPRKHIEYRGVTLEEMDTEAIIARHPKVALVDELAHTNVPGSKH
                     PKRYQDVLEILDAGIDVVTTLNIQHLESLNDLVASITGVRVRETLPDWILDQADEVEL
                     IDISPYALRQRMKHGNIYPRERIEAALNNFFREGNLTALREMALRRTAEKTESQLQAY
                     MTEHGIKEIWPASERVLVGFDHRPHTRQVIRDAWRLAHGLHADLIAVSIQPEGYLAFT
                     SKLIGWLKYRGEAKQQRAAALQRLEEHAVLAEDLGAEVIRTSSGDIARKLVEIARERQ
                     ATQLVLGQPARSHWEELIRGSII"
     gene            complement(1352..>1598)
                     /locus_tag="VGT44_14515"
     CDS             complement(1352..>1598)
                     /locus_tag="VGT44_14515"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HEV2582066.1"
                     /translation="LPVPSGPVPVDLVTTSGSGLDPDISIAAALYQVPIVAAARGLSQ
                     DVVTQLVTSHETGRFLGIFGEPYVNVLDLNLALDALK"
BASE COUNT          251 a          493 c          517 g          337 t
ORIGIN      
        1 tgatgatgga gccgcgaatg agttcttccc aatggctgcg cgccggctgg cccagcacca
       61 gttgcgtcgc ctggcgctcg cgagcgatct cgaccagctt ccgcgcgatg tcaccgctgc
      121 tggtgcggat gacttcggcc cccaggtctt cggcaagcac ggcatgttct tccaggcgtt
      181 gcagcgccgc cgcgcgctgc tgcttcgcct ctccacgata tttgagccag ccgatgagct
      241 tgctggtgaa ggccaggtag ccctccggct ggatcgagac ggcgatcagg tcggcgtgca
      301 ggccgtgtgc cagccgccag gcgtcgcgga tgacctggcg cgtatgtggc cggtgatcaa
      361 agcccaccag cacccgctcg ctggctggcc agatctcctt gatgccgtgt tcggtcatgt
      421 atgcttgcag ttgggactcg gtcttttcgg cggtgcgccg cagagccatt tcgcgcaaag
      481 cggtcaggtt gccctcgcga aagaagttgt tgagcgccgc ctcgatgcgc tcgcgcgggt
      541 agatgttgcc gtgtttcatg cgttgtcgca gggcgtaggg ggagatatcg atcagctcta
      601 cttcgtcggc ctggtcaaga atccaatcgg gcagcgtttc acgcacgcgc acaccggtga
      661 tgctcgctac caggtcattg aggctttcca ggtgctggat attcagcgtg gtgacgacat
      721 cgatacccgc gtcgaggatt tccagtacgt cctggtagcg cttgggatgc ttggagccgg
      781 gtacgtttgt gtgcgccagc tcatcgacca gtgccacctt ggggtgccgc gcgatgatgg
      841 cctcggtgtc catctcctcg agcgtcacgc cccggtattc gatgtgcttg cgtggaatga
      901 tcttcagatc cccgatttgt gcctcggtct gggaccgcgc gtgcgtctcc acgtagccga
      961 tcaccacgtt ggcgccccgg ccctcacggc gatgcccctc gttcagcatg gcgtaggtct
     1021 tgcccgaacc agctgccgag gccaggtaga cgcgcagcct cccgcgtcgt cgcgtgtagc
     1081 cagcggcagc tggctggggt acgacgagag cgtcggagcc tggcacaggc gatgccgggg
     1141 gagcctccag gtcgctatcg cgcaggctgt agcgccgcag gagttcctcc gggtcgggcc
     1201 gtccttcttc gcgtgcgcgg ggttcgtgag gcgattgctc ggccatcgtc gatgatcctt
     1261 ttgttcaacc tactttagtg tatatgcaat ccatcagcct accgttcgta ggagcgcggt
     1321 aaaccggctc ctacggtgtt cctttgcggc atcatttcag cgcgtccagc gccaggttca
     1381 ggtcgagtac gttgacgtag ggttcgccga aaatgcccag gaagcgtccc gtttcgtgag
     1441 acgtgaccag ttgcgttact acatcctggc tcagcccgcg cgcagctgcc acgatcggaa
     1501 cctggtacag cgccgccgca atcgagatgt ccgggtccag gccggaaccg gaggtcgtca
     1561 caagatcaac aggcacggga cccgaaggca ccggtaga
//
LOCUS       DASZAK010000824         1867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_482358, whole genome shotgun sequence.
ACCESSION   DASZAK010000824 DASZAK010000000
VERSION     DASZAK010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482358"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(437..>1867)
                     /locus_tag="VGT44_14520"
     CDS             complement(437..>1867)
                     /locus_tag="VGT44_14520"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV2582067.1"
                     /translation="LLFPQSFLRSMGMGAIAATLVALIAALTILPAVLALLGRRINAL
                     SLQRLFRRRNTAQRGNTETQGVWYRLSEMVMRQPIIVALVVLAMLVTLGLPFLHASYS
                     TPDVRVLPADQQARIVSDQLSLDFAQQGNAQLTIAVTTHGEVLSAANLANLDNYVQSI
                     ERIPGVITVNSLVTVSPTLTLANYQQLYAHPDANPQFSAIAAQLANGNVTKITVAIQP
                     ADHSAAAQDIVRQVRALPTPGGIIPMVDGTTAGQIDLLASLGATLPYALLVMAVAVLV
                     LLFLMTGSLLMPLKAILLNILSLSATFGGLVWIFQDGHLQNLLGFTSTGSIDATQPIL
                     IFAIAFGLSMDYEVFLLSRIKERFDATGDNRAAVSSGLQRTGWLITSAALLLAVVVGA
                     FGSSKIVFIQEIGIGLAIAVIIDATLVRMLLVPATMRLLGNWNWWAPAPLKALWRFVG
                     LGESTAPRRAVNEVEASPAEREIAQV"
BASE COUNT          364 a          489 c          644 g          370 t
ORIGIN      
        1 catattcagg catgaaatca ttcccctctc tcttttttac tgctgtattg tactcgcttt
       61 ttgcgtagag tggaaggatt tctgttgtgc agttcaaagt ccttgctctg ggtggggagt
      121 gtggcggacg gatgtaggag atagttgctg gcaagcaagc gtcagaatcc ctgaaggagc
      181 cgatgatgaa tgttcaccac cagggtcggt catgccatcc tgagcgcagc gaaggatctg
      241 actcgccgag gaccgagatc cttcgctgcg ctcaggatga caacactaat catgtttatg
      301 aaacttcatc atcggccagt ccagccgttc ggatcgacga ggattcgcgc cgatcaatcg
      361 gcggcgagcg cggtaaaccg gctcctactg ttttgtcaag gttgattgat ggcctacaac
      421 gattccatca agttgattac acctgtgcga tttctcgttc ggctggtgag gcttccactt
      481 catttaccgc tcgccgggga gcggttgatt cgcccagacc cacgaagcgc catagggctt
      541 tgagcggcgc tggcgcccac cagttccagt tgccgagcag gcgcatggtg gcggggacga
      601 gcagcatgcg taccagggtg gcgtcgatga tgacggcgat ggcgaggccg atgccgattt
      661 cctggatgaa gacgatcttc gagctgccga atgcccccac tacgacggcc agcagcaggg
      721 cggcgctggt gatgagccag ccggtgcgct gcagaccgga tgagacggcg gcgcgattat
      781 cgccggtggc gtcgaagcgc tctttgatgc ggctcagcag gaaaacctcg tagtccatcg
      841 agagaccgaa ggctatggcg aagatcagga tgggctgcgt ggcatcgatg ctaccggtcg
      901 aagtgaagcc cagcaggttt tgcaggtggc cgtcctggaa aatccagacc aggccgccaa
      961 aggtggccga gagcgagagg atgttcagca ggatggcctt gaggggcatc aacagcgagc
     1021 cggtcatgag gaagagcagc accaggacgg cgacggccat caccagcagg gcatagggca
     1081 gcgtcgcgcc caggctggcc agcaggtcga tctgccccgc ggttgtgcca tccaccatgg
     1141 ggatgatgcc gccgggagtt ggcagggcgc gcacctggcg cacgatgtcc tgcgcagctg
     1201 cgctgtggtc ggcgggttgg atggcgacgg tgattttcgt gacgttgccg ttggccagtt
     1261 gcgctgcgat tgccgagaac tgtgggttgg cgtcgggatg cgcgtagagc tgctggtagt
     1321 tggcgagcgt cagggtgggc gagacggtga cgaggctatt gacggtaatc acacctggaa
     1381 tgcgttcgat ggattggacg tagttgtcca ggtttgccag atttgctgct gataatacct
     1441 cgccatgcgt agtgacggct attgtcagtt gggcgttacc ctgctgggcg aagtccaggc
     1501 tgagctggtc tgaaacgatg cgcgcctgct ggtcggcggg cagcacacgc acatcgggcg
     1561 tggaatatga ggcgtggagg aatggcaggc cgagcgtgac gagcatcgcc aggacgacca
     1621 gcgcgacgat gatgggctgg cgcataacca tttccgagag ccggtaccac acgccctgcg
     1681 tttcagtatt gccgcgttgc gccgtgtttc tccggcggaa gagccgctgc agggagagcg
     1741 cgttgatgcg cctgccgagc agcgccagga ccgccgggag aatggtgagc gcggcgatta
     1801 aggcaacgag cgtggcggcg atcgcgccca tgcccatgct gcgcaggaac gattgcggga
     1861 agagcag
//
LOCUS       DASZAK010000825         2043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_552913, whole genome shotgun sequence.
ACCESSION   DASZAK010000825 DASZAK010000000
VERSION     DASZAK010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552913"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2043
                     /locus_tag="VGT44_14525"
     CDS             <1..>2043
                     /locus_tag="VGT44_14525"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF009687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV2582068.1"
                     /translation="TYRTIDDLSRRTSQWSFIDLYRKGLVYRREAPTIWCSECRTAIA
                     QAELNDLERDSEFVTLPFHLENGDILPIATTRPELLPACVAVFVHPDDARYAHLAGQR
                     IAVPLLGFDVPLLVDAKADPQKGTGAVMCCTFGDATDVEWWYTHKLPLKMLIGRDGRM
                     TSAAGDLVGLSIPQARQRMIALLDAQGLLLARQPTTQTVRVHERCDTPAEYLVTQQWF
                     VRVLDYKEDLLAAGEQIEWHPAHMKARYRAWVENLGWDWCISRQRAFGVSFPLWYCDA
                     CGEILLADEDQLPLDPTERQPSRACACGNTTFTPEEDVMDTWATSSMSPQIVGRWLVD
                     PDLYGQVFPMSLRPQAHEIIRTWAFDTIVKSYHHFGALPWKNIAIAGWGVAAAGAGKI
                     SKSRGGGPMPPMAMIEKYSADAVRYWAASAGPGKDAIISEEKIMLGGKLITKLWNVAS
                     FSQRFLDGYQPPRDSSKASLAFTPTDRWLLSNLQHLIVHVTEHFRQYDYAAARNEAEG
                     FFWRVLADNYLEFAKERLYDEAHPLREGARYTLYHALLATLKLFAPFFPYVTETIYRE
                     LFAASDGSDSIHASPWPTANETLIDDDAEASGELLIEIATAVRRYKSEASLSLGAELQ
                     RLRLSTGSPAEADMLRAASVDIRSVTRAKVVEVGETGEEGGNGWEMIKDVGRVMVLL"
BASE COUNT          378 a          633 c          674 g          358 t
ORIGIN      
        1 acctatcgca ccatcgacga tctatcgcgg cgcacgtcgc agtggtcctt catcgacctg
       61 taccgcaagg ggctggtcta ccgtcgcgaa gcgccgacca tctggtgttc cgaatgccgc
      121 accgccatcg cgcaggccga actgaacgac cttgaacgcg acagcgagtt cgtcacgctg
      181 cctttccacc tggaaaatgg cgatattctg cccatcgcca cgacgcgccc ggaactgcta
      241 ccggcctgcg tggcggtctt cgtgcatccc gatgatgccc gctatgcgca cctggcaggc
      301 cagcgcatcg cggtgccgct gctcggcttc gatgtgccgc tgctggtgga cgcgaaggcc
      361 gacccgcaga agggtaccgg cgcggtgatg tgctgcacct ttggcgacgc gaccgatgtg
      421 gaatggtggt atacgcacaa actgccgctc aagatgttga tcgggcgcga cggcaggatg
      481 accagtgccg ccggagacct ggtcggcctg agcatcccgc aggcacggca gcgcatgatt
      541 gccttgctgg acgcgcaggg gctgctgctg gcgcgccagc cgaccacgca gacggtgcgg
      601 gtgcatgaac gctgcgatac cccggcggaa tacctggtga cgcagcagtg gttcgtgcgc
      661 gtgctggatt ataaagaaga tttactggcg gcgggcgagc agatcgaatg gcaccccgcg
      721 catatgaagg cgcgctaccg ggcctgggtg gaaaacctgg ggtgggactg gtgtatctcg
      781 cgccagcgcg ccttcggcgt gtcattcccg ctgtggtact gcgatgcctg cggcgaaatc
      841 ctgctggccg acgaagatca actgccgctt gacccgaccg agcggcagcc ctcacgcgcc
      901 tgcgcgtgcg gcaatacgac gttcacgccg gaggaagatg tgatggatac ctgggctacc
      961 tcgtcgatgt cgccgcagat cgtggggcgc tggctggtcg atcccgacct gtacgggcag
     1021 gtcttcccga tgagcctgcg tccccaggcg cacgagatca tccgcacctg ggccttcgat
     1081 accatcgtga agtcctacca tcactttggc gcgctgccct ggaagaacat cgccatagca
     1141 ggctggggcg tggctgccgc cggggcgggc aagatcagca agagtcgcgg cggcggcccc
     1201 atgccgccga tggcgatgat cgagaagtat tccgccgatg ccgtgcgcta ctgggccgcc
     1261 agcgccgggc cgggcaagga cgccatcatc agcgaggaga aaatcatgct gggcggcaag
     1321 ctcatcacca agctctggaa tgtggccagc tttagccagc gcttcctgga tggctaccag
     1381 ccgccgcgcg actcgtccaa ggcatcgctc gccttcacgc ccaccgatcg ctggctgctt
     1441 tccaatttgc agcatctgat cgtgcatgtc acggagcatt tccggcaata cgattacgcg
     1501 gcagccagaa acgaggccga gggctttttc tggcgcgtgc tggccgacaa ctacctggaa
     1561 ttcgcgaaag agcgactcta cgacgaagcg caccccctgc gcgaaggggc gcgctacacg
     1621 ctctatcacg cgctgctggc gacgctcaaa ctgttcgcgc ccttcttccc gtatgtgacg
     1681 gagacgatct atcgcgaact cttcgctgcg agcgatggca gtgactcgat ccacgccagt
     1741 ccctggccca ccgccaacga gacgctcatc gacgacgatg cagaagcatc gggcgagctg
     1801 ctgatcgaga tcgctactgc cgtgcggcgc tacaagagcg aagccagcct ctcgctcggt
     1861 gcggaactgc aacgcctgcg cctttcgacg ggaagccctg ctgaagcgga tatgctgcgc
     1921 gccgccagcg tggatatcag gagtgtgacg cgggcgaagg tggttgaggt gggggagacg
     1981 ggagaggaag gtggcaatgg gtgggagatg attaaggacg tggggagggt gatggtgctg
     2041 cta
//
LOCUS       DASZAK010000826         4037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_626135, whole genome shotgun sequence.
ACCESSION   DASZAK010000826 DASZAK010000000
VERSION     DASZAK010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_626135"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VGT44_14530"
     CDS             <1..410
                     /locus_tag="VGT44_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582069.1"
                     /translation="RPLVVRISLMILRLAVLINLVLGILFWINTIDPDANTGIKLLHI
                     VLGILAVISLFTIGIAYGLRGGSFGLALATFVVGFLLTFVGLFQQNWLTAPDQPHWII
                     QIIHLVLGLSAIGLGERIGRQYNHMVRAKAKTA"
     gene            499..1008
                     /gene="coaD"
                     /locus_tag="VGT44_14535"
     CDS             499..1008
                     /gene="coaD"
                     /locus_tag="VGT44_14535"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV2582070.1"
                     /translation="MNNQDNRTPRIAVYPGTFDPLTNGHLDIARRAARLFDTLIVSVY
                     TFPSKNLLFTVEERMALWQEVIAAEDLPNVRVDMYKELTVNYVRSVGGQAIVKGLRSP
                     NDFEAEFQQGLMNRKLAPQIETVCLLTNLEQLFVSSSLLKEVARLGGDVTDMLPPPVI
                     AALRKKFGE"
     gene            1037..1705
                     /locus_tag="VGT44_14540"
     CDS             1037..1705
                     /locus_tag="VGT44_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582071.1"
                     /translation="MDILYLVDRLENLIASSRRMPLFNQIMVKEADMLDIIETMRTAI
                     PDEIKQARRIIQEKERILAQAQAEASSLLAKAREESEKAMKRDGLLRAAEERSETMLR
                     QAEEKAERLKIEADAYVVETLRALHEHLSGVEMEIDRSLISIEKGLESMEEHGEIEMG
                     DNDEEEHDPPYLDDGTNATQDRQDSPGAKRVQQADTTPRPGQQPRRALLAVDTMGGPM
                     LPPS"
     gene            1842..2381
                     /locus_tag="VGT44_14545"
     CDS             1842..2381
                     /locus_tag="VGT44_14545"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV2582072.1"
                     /translation="MDSLIYNVAQLLKSPVGTSVAYDFSEGDVQLEDIRTAGPLNGHA
                     RMRRTNQGLLIDGWVDLTLALTCDRCLKEFERPVHVTFEEQFYPTVDVISGLPLAKFD
                     AEEIFPINAHHEVDLTEAIRQNTLTKLPMVTLCREDCQGLCSQCGQDLNLGPCECAPE
                     VVDSRMSVLEQLLREQKLS"
     gene            2448..2639
                     /gene="rpmF"
                     /locus_tag="VGT44_14550"
     CDS             2448..2639
                     /gene="rpmF"
                     /locus_tag="VGT44_14550"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV2582073.1"
                     /translation="MGALPKQRTSHARQGNRRAHHHLKLPQLVACPNCRKPRLSHHAC
                     PNCGMYRGRQIFIPRTKKS"
     gene            2701..3729
                     /gene="fabD"
                     /locus_tag="VGT44_14555"
     CDS             2701..3729
                     /gene="fabD"
                     /locus_tag="VGT44_14555"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV2582074.1"
                     /translation="MTQVAFLFPGQGSQAVGMGADLYASSPAARRVFDAVDEALGFSL
                     SSLCFNGPEDTLRETINAQPAIVTVSLALLAALQEALSSSSSSSWTAPLAPHFTAGHS
                     VGEYAALVAAGALSLADAARLVRERGRLMHHEGTVCPGGMAAVIGLDEEPLQQICQDV
                     TAQRTANLPAASHPGLGQVTVANFNAPGQIVISGEQGALEAAMAEARARGAKRAIPLA
                     VSAAFHSPVMQPATTGLAQAIAATQVNDAAVPVVGNIHAQTLTQADEIREELAQQVAA
                     SVQWIRTIEFMANAGVTVFLEIGPGQALTGMVKRIAKGVTTLNVSNATDLEKTVRNLR
                     EMHVSDSV"
     gene            3840..>4037
                     /locus_tag="VGT44_14560"
     CDS             3840..>4037
                     /locus_tag="VGT44_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582075.1"
                     /translation="MVTESPGVVRVARQVLLSIITNVTIEIPGVTRMAQGGDPWSRLL
                     GREIPRTGIALTIKENTLSADL"
BASE COUNT          915 a         1140 c         1149 g          833 t
ORIGIN      
        1 aaagaccatt ggttgttcgt atctcactca tgatactacg tctcgccgta ctgattaacc
       61 tcgtgctggg catcctgttc tggataaaca ccatcgatcc cgacgctaat acgggcatca
      121 agttgttgca catcgttctc ggcatcctgg cagtcatctc gctcttcacc atcggcatcg
      181 cgtatggctt gcgcggcggc agctttggcc tggcgctggc taccttcgtc gtcggcttcc
      241 tgctgacctt cgtcggacta ttccagcaaa actggctgac cgcgcctgac cagcctcact
      301 ggatcattca gatcattcac ctcgtcctcg gcctgtccgc gattggcctg ggcgagcgga
      361 tcggcagaca gtacaaccac atggtcaggg caaaggcgaa aaccgcgtaa tgcgctattg
      421 ccgaatgctt gccgatatgg ttaaatggag acaactttca atctaccagc cttgtaatat
      481 tttcgaggga agtaaagcgt gaataatcag gacaatcgca ccccgcgtat cgctgtttat
      541 ccaggcacat ttgacccact caccaatgga catctcgata tcgcgcggcg ggcagcgcgc
      601 ctgttcgaca ccctgattgt ttcggtgtat acatttccct cgaagaacct gctctttacc
      661 gttgaagagc gtatggcact ctggcaggaa gtcatcgccg ccgaggattt gcccaatgtg
      721 cgcgttgata tgtacaagga gctgaccgta aactacgtgc gctcggtagg agggcaggcc
      781 atcgtcaagg gcttacgttc gcccaacgac ttcgaggccg agttccagca gggactgatg
      841 aaccgcaaac tcgcgccgca aatcgaaacg gtctgcctgt tgaccaatct agagcaattg
      901 tttgtcagtt cgtcgttatt aaaagaggtg gcgcgcctgg gaggagacgt gacggatatg
      961 ctgccgcctc ccgtcatcgc cgctttgcgc aaaaaatttg gagagtagcg cgtcttgttg
     1021 aaatcggagg agaggcatgg atattttata ccttgtcgat cgcctggaaa atttgatcgc
     1081 gagcagtcgc agaatgccgt tgttcaacca gatcatggtc aaggaagcgg atatgctcga
     1141 tatcatcgag acgatgcgca ccgccatccc cgatgaaatc aagcaggccc gccgtattat
     1201 ccaggagaaa gaacgcatcc tggcgcaagc gcaggcggaa gccagctccc tgctcgcgaa
     1261 ggcgcgcgag gagtccgaga aggccatgaa gcgcgatggt ctgctgcgcg ccgccgagga
     1321 acgttccgaa acgatgctgc gtcaggcaga ggaaaaggcc gagcgcttga agatcgaagc
     1381 cgatgcctat gtcgtggaaa ccctgcgcgc cctgcacgaa cacctttcgg gtgttgaaat
     1441 ggaaattgac cgctcgctga tcagtatcga gaaaggtctt gagagcatgg aggagcatgg
     1501 cgaaatcgag atgggggaca acgacgaaga ggagcatgac ccgccctatc tcgatgacgg
     1561 aacgaacgcc acccaggacc gccaggactc acctggcgca aagagggtcc agcaagccga
     1621 cactacgccg cgaccgggtc agcagccgcg acgcgcattg ctggcggtcg atacaatggg
     1681 cgggccgatg ctcccacctt cctaaaagac ttgacacaca ttgattggtc ttctataatg
     1741 aacaagcatt cgctagcgcc aggatagaat cgcttgtatg gttgcgccac aaccatattt
     1801 ttttgtgggt tggtttgcaa tgaagggata aagaaagcag tatggatagc ttgatttaca
     1861 atgtggcgca actgttgaag tccccggtcg gaacctcagt ggcctacgat ttttccgaag
     1921 gagatgtaca actcgaagat attcgcacgg caggcccgct gaacgggcac gcacgaatgc
     1981 gccgcacaaa ccagggcctg ctgatcgatg gttgggtcga tctcacgctg gcattaacgt
     2041 gcgaccggtg cttgaaggaa ttcgagcgcc cggtgcatgt gacgtttgaa gaacaattct
     2101 acccaaccgt agatgttatc agcggcctgc ctctggcaaa gttcgacgcg gaagaaatct
     2161 tccccatcaa cgcgcatcac gaggtagatt taaccgaagc cattcgccag aatactctga
     2221 cgaagttacc gatggtcacg ctgtgccgcg aagattgcca ggggctgtgt tcgcaatgcg
     2281 ggcaggacct caatctggga ccgtgcgagt gtgcgccaga agtcgtcgat tcgcgcatga
     2341 gtgtgcttga gcagttgctg cgagagcaaa aactctcttg aatagtgcct atggcggtct
     2401 ccatgcgctc gcgcacatcg tatagatttt agaggaggaa tatcattatt ggagcgttac
     2461 ccaaacaacg aacttcccat gctcgccagg gtaatcggcg agcgcatcat catctcaaac
     2521 tgccacagct ggtagcgtgt ccaaattgcc ggaaaccccg gctgtcgcac cacgcctgcc
     2581 cgaattgtgg catgtatcgc ggtcgtcaga ttttcatacc gcgcacaaag aagagctaag
     2641 gacaacacga aaagcgaggc cggttgcatc aacccgcctc cttttcataa gcgagtgaat
     2701 atgacgcaag tcgcctttct ttttcctggc cagggcagcc aggcagtcgg catgggggcc
     2761 gatctctacg cctcgtctcc cgccgctcgg cgcgtatttg acgcggtaga cgaggcattg
     2821 ggcttttcgc tgagttcgct ctgtttcaac ggaccggaag atacgctgcg cgaaaccatc
     2881 aacgcccaac ctgcgattgt caccgtcagc ctcgcgctgc tcgcagcttt gcaagaagct
     2941 ctttcttctt catcgtcctc atcgtggact gctccgctgg ctccccattt caccgccgga
     3001 cacagcgttg gcgagtacgc cgcgttggtc gctgcaggcg cgctgtcgct cgcagatgcc
     3061 gcgcgcctgg tgcgtgaacg cggacgcttg atgcatcacg agggaaccgt ctgccccggc
     3121 ggcatggccg ccgtgatcgg cctcgatgaa gagccattac agcagatctg ccaggatgtt
     3181 accgcgcagc ggaccgccaa tcttcccgcc gcgtcacacc ccggcctggg gcaagtcacc
     3241 gtcgcgaact tcaacgcgcc cggccagatt gtgatctcag gcgaacaggg cgcgttggaa
     3301 gcagccatgg cagaggccag ggcgcgcggg gcgaaacggg ccatcccgct ggcggtcagc
     3361 gccgcgtttc actcgccggt catgcagccc gcgacaaccg gcctggcgca ggccatcgcc
     3421 gcgacgcagg tcaacgacgc tgccgttccc gtcgtgggca acattcacgc gcagacgctg
     3481 acacaggccg acgagattcg cgaggaactg gcgcagcagg tcgccgcgtc ggtgcaatgg
     3541 atacgcacca tcgagtttat ggcgaatgcg ggtgtcaccg tttttcttga aatcggcccc
     3601 ggacaggccc tgaccggcat ggtcaaacgc atcgcaaagg gcgtcaccac gctgaacgtc
     3661 agcaacgcga ccgacctgga gaaaaccgtg agaaacttgc gtgaaatgca tgtatccgat
     3721 agcgtataat ctgtaacaag ggggaggcgg gggcaattgc ctccagcagt gccaaaaact
     3781 tgacaatgta tttcttacat gcctatcctt gcagtaaggt gttttagctg aggaaatcga
     3841 tggtgacaga atcgccagga gtagtacggg tagcgcgcca ggtattgctc tcgattatta
     3901 caaatgtgac gatagaaata ccgggcgtta cgcgtatggc gcaaggcggc gatccctggt
     3961 cgcgtttgct tggccgcgaa attccccgca ccgggattgc gttgaccatc aaagagaaca
     4021 cactctccgc cgatcta
//
LOCUS       DASZAK010000827         4847 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_233739, whole genome shotgun sequence.
ACCESSION   DASZAK010000827 DASZAK010000000
VERSION     DASZAK010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233739"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VGT44_14565"
     CDS             <1..1083
                     /locus_tag="VGT44_14565"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV2582076.1"
                     /translation="DLMAEVLSALVKRAGIDAGLVDDVIIGCVIQAGEQALNVGRNAV
                     LAAGFPESVPGTTVDRQCGSSQQAIHFAAQGVLSGAYDIVIAGGVEVMSRVPMGASTV
                     NGPGAPFGPRMLKRYDNGLVHQGISADLIARKWEISREELDAYSLESHRRAAQATAEG
                     RFRSQILPVEVKNEDGTTAIFDKDEGIRPDTSLEKLASLKPSFKPDGLITAGNSSQIS
                     DGAAAVLIMDRETAERLGLRPRARFVSFALAGDDPIFMLTAPIPATKKALRKANMTLD
                     QIDLVEINEAFASVPLSWQRETGADMSKVNVNGGAVAVGHPLGASGARLTTVLLNELE
                     RTGGRFGLQTICEGGGMANAMIIERI"
     gene            1087..2184
                     /locus_tag="VGT44_14570"
     CDS             1087..2184
                     /locus_tag="VGT44_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2582077.1"
                     /translation="MPNFAPTEEQEEIRSLARSLAVEQLRAQGRASEKQGDISPALAD
                     TLAQTGLTAPFPEQLGGSGAIEAMTYTLIAEELGYGDGALAINIIGSLMGPLAVLLAG
                     GAGQQQTYITPFCDTRTGPVQRGSFAYAERTGGYTLADVTASVRKEGDAYIVNGAKRD
                     VIHGTNSSPRVALLRLEGTSGLDGLCALMVPAQGEGITLRNDTLKLGLTAAPSASFDF
                     VNAQVMASCMLGQPGDRGVIRAATLYNILRAGVACGMARAALEYARDYAKDRIAFGRP
                     IASYQGIAFMVAEMAMNLDAARLLVWRAAVEWDGDTALETLVHDAEAAQHQAVKIAKS
                     ATIDGIQILGGAGFIQDHPVEMWARNAAAME"
     gene            2199..3383
                     /locus_tag="VGT44_14575"
     CDS             2199..3383
                     /locus_tag="VGT44_14575"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2582078.1"
                     /translation="MINFTLSKSQQAARDYAHQVALEEMRPLSLECDRTEKIPESFFW
                     NMQKRFWAGASGQARMQAQEDEEKQSNVLSVASQEEMAWGDAALSTAIPGPGLAAPPI
                     LSQGTPEQQMRFLGPYMDGQLHWGALALTEPGIGSDVAGMSTTATLDGDEWVINGHKH
                     YITNGARADLVVTFATVDMRLGREGIRPFVVPKGTPGMIVGRIEEKMGLRASQTAELI
                     YENCRVPQDHLLAGREKTQKAGFKAAMGTLDATRPMVGALAVGIARAAHETTREWVCD
                     ELPKGFLAHKRREIEDELEELGQQIEMARFLVWRAAWMADKRIPNSKEASMSKAFAGA
                     LVMKVTAAGVRISAPGENSERRQFLAKWFRDAKIFDIFEGTAQVQRLVIARRLFPEVD
                     IP"
     gene            complement(3442..3633)
                     /locus_tag="VGT44_14580"
     CDS             complement(3442..3633)
                     /locus_tag="VGT44_14580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582079.1"
                     /translation="MRGKLRDKRADARRDRMRRELVERRRSPKRENRTAAFLNRQLAD
                     EYVLEEEEEYAGAVVAGKK"
     gene            4258..4734
                     /locus_tag="VGT44_14585"
     CDS             4258..4734
                     /locus_tag="VGT44_14585"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEV2582080.1"
                     /translation="MRNNTHHSESAFKPFNGFWFGLAALALIVILAACGGTSTGSPPA
                     SGGTTPTATSGGGSTPTTAPSGNTVAVTITTDSSGSFAFSPAAVTIKAGTTVVWTNTT
                     GAPHTVTSDDGKTFDSGIANPIPASGGTFKFTFNTTGSFSYHCQIHPYMKATIVVQ"
BASE COUNT          981 a         1521 c         1505 g          840 t
ORIGIN      
        1 gatttgatgg ctgaggtgtt gtctgcgctg gtgaagcgcg ccggcattga cgcgggtctt
       61 gtagacgatg taattatcgg atgtgttatc caggcgggcg agcaggcgct caacgtgggg
      121 cgcaacgccg ttctcgcggc gggcttcccc gaaagcgtgc cggggacgac cgttgaccgc
      181 cagtgcggat cgagccagca ggccatccat ttcgcggcgc agggcgtcct cagcggcgcg
      241 tatgatatcg tcatcgcggg tggagtcgag gtcatgtcgc gtgtcccgat gggagcaagc
      301 accgtcaacg gacccggtgc gccatttgga ccgcgtatgt tgaaacgcta cgacaacggg
      361 ctggttcacc agggcatcag cgccgacctg atcgcgcgca aatgggagat cagccgcgaa
      421 gaactggacg cctacagcct ggagagccat cgccgcgccg cgcaggccac tgccgaggga
      481 cgtttccgca gccagattct ccccgttgag gtcaagaacg aggatggcac caccgctatc
      541 tttgacaaag atgagggtat tcgccccgat acaagcctgg agaagctggc aagcctcaaa
      601 ccctccttca agcctgatgg cctgattacc gccggcaact cctcgcagat cagcgatggc
      661 gcggcggcag tgctgattat ggatcgcgag accgccgagc ggctgggact gcggccccgc
      721 gcgcgcttcg tctccttcgc cctggccggg gatgacccca tcttcatgct gaccgcgccc
      781 attcccgcga cgaaaaaggc gctgcgcaag gcgaatatga cgctcgacca gatcgacctc
      841 gtggagatca acgaagcttt cgccagcgta ccgctctcct ggcagcgcga gaccggcgcg
      901 gacatgtcga aggtcaacgt caacggcggc gcggtcgcgg tcggccaccc cctgggcgcc
      961 tccggcgcgc gcctgaccac ggtgctgctg aacgaactgg agcgcaccgg cggacgcttc
     1021 ggcctgcaaa ccatctgcga gggcggcggc atggccaacg ccatgattat cgaaaggatc
     1081 tagtgcatgc ccaacttcgc cccaacggag gagcaagagg agattcgcag cctggcgcgc
     1141 agcctcgccg tcgagcaact gcgcgcccag ggccgcgcct ctgagaaaca aggcgacatc
     1201 tcacccgccc tggcggacac gctggcgcag accggtctga ccgcgccctt cccggagcag
     1261 ttgggcggct ccggcgcaat cgaggccatg acctacaccc tcatcgcgga agagttgggc
     1321 tacggcgacg gcgcgctggc tatcaacatc atcggctccc tgatggggcc gctagccgtg
     1381 ctgctggcgg gcggcgcagg ccagcagcag acctacatca cccccttctg cgacacgcgc
     1441 accggcccgg tgcagcgcgg cagcttcgcc tacgccgaac gcaccggcgg ctacacgctg
     1501 gccgatgtca cagcgtcagt gcgcaaagag ggcgatgcct acatcgtcaa cggcgcgaag
     1561 cgtgacgtga tacacggcac gaactccagc ccgcgcgtcg cgctgctgcg cctggaaggg
     1621 acgagcggac tggatgggct gtgcgcgctg atggttcccg cgcaaggcga aggcatcacg
     1681 ctgcgtaacg acacgctgaa actcggcttg accgccgctc cgagcgcgtc gttcgacttt
     1741 gtgaatgcgc aggtgatggc gagctgtatg ctgggtcagc caggcgatcg cggcgtgatc
     1801 cgcgctgcca cgctctacaa catcctgcgc gcgggcgtgg cctgcggcat ggcgcgcgcg
     1861 gcgctcgaat acgccaggga ctacgccaag gaccgcatcg cctttggccg acctatcgcc
     1921 tcctaccagg gcatcgcctt catggtggcg gagatggcga tgaacctgga cgcggcgcgc
     1981 ctgctggtct ggcgcgcggc ggtcgagtgg gacggcgata cagcgctgga gacgctggta
     2041 catgacgcag aggcagccca gcaccaggcc gtcaagatcg ccaaatcagc gactatcgac
     2101 ggcatccaga ttctgggcgg cgcgggcttc atccaggatc accccgtcga aatgtgggcg
     2161 cgcaacgcag cagccatgga gtgaggagcg cataaacgat gattaatttt acactgagca
     2221 aatcacaaca ggcggctcgc gactacgcgc accaggtggc gctggaagag atgcgcccac
     2281 tctcgctcga atgcgaccgc acggagaaaa tccccgaatc gttcttctgg aatatgcaaa
     2341 aacgcttctg ggcgggcgcg tccggccagg cgcgcatgca ggctcaggag gacgaggaga
     2401 agcagagcaa cgtgctgtcg gtcgcttcgc aggaagagat ggcctggggc gatgccgcgc
     2461 tctcgaccgc cattcctggg ccggggctgg cggctccgcc catcctctcg cagggaacgc
     2521 ccgaacagca gatgcgcttc ctggggccat acatggacgg gcaactgcac tggggcgcgc
     2581 tggcgctgac cgagccgggc atcggctccg acgtggcggg catgtccaca acggccacgc
     2641 tggacggcga cgagtgggtc atcaatggcc acaagcacta catcaccaac ggcgcgcgcg
     2701 ccgacctggt tgtaaccttc gccaccgtcg atatgcgcct ggggcgcgag ggcatccgtc
     2761 ccttcgtcgt ccccaaaggc acgcccggca tgatcgtcgg gcgcatcgag gagaagatgg
     2821 gtctgcgcgc ctcgcagacc gccgaactga tctacgagaa ctgccgcgtg ccgcaggacc
     2881 atctgctagc cgggcgcgag aagacgcaga aggcgggctt caaggctgct atgggaaccc
     2941 tcgacgcgac gcgcccgatg gtcggcgcgc tggcggtggg catcgcgcgc gccgcgcacg
     3001 aaaccacgcg cgagtgggtc tgcgatgagc tgccaaaggg ctttctcgcc cacaaacggc
     3061 gcgagatcga ggacgagctg gaggaacttg gccagcagat cgagatggcg cgcttcctgg
     3121 tgtggcgcgc ggcatggatg gccgacaaac gcatccccaa ctcgaaggaa gcctcgatga
     3181 gcaaagcctt cgcgggtgcg ctggtcatga aggtcacggc ggcaggcgtg cgcatcagcg
     3241 cgccgggcga aaattccgag cgcaggcaat tcctggcaaa gtggttccgc gacgcgaaga
     3301 tcttcgacat cttcgaggga accgcgcagg tgcagcgcct ggtgatcgcg cgccgcctct
     3361 tcccggaggt ggatatacca taagcaataa accaatcgac accagccccc ggtcgggggc
     3421 tggtgtcgat tggtttattg cttacttctt gcctgccact acagcgcctg cgtattcctc
     3481 ttcttcttcc agcacatact catccgctag ctggcgattg aggaacgcgg ctgtgcgatt
     3541 ctctcgcttc ggggaacgac ggcgttccac cagttcgcgc cgcatgcggt ctcgccgggc
     3601 atcagcgcgt ttatcgcgga gttttcctct cacaccttca cctcctctct gctcaagatc
     3661 gccggattgt gtttctgggc catgggacga tgcgcgtcac gcacgtttgc gcctttctgc
     3721 tcgcacggga tagcgcccgg cgaaggctgg ttgctgtaga acaaagttga acaatacaca
     3781 gggtaaacga ttcatagcgc tttctctatc gtaggagatt caatgtgagg cgtccggcgc
     3841 aaaacgtggg aactaagagg tgggaatcgt gttcgcattc acgtggagca tgatgcgggt
     3901 caacattctg aacgatcata cagagaggaa ctaccttgat ctgttgaacg gatgagttcc
     3961 tgcattgagc gccatcagcc tgatacaagc agacatttgc ttcatagaac tcaccccctt
     4021 taaatatgcc tggtcaacgt caataagccc tattagctca tctgcctaga gtgtacaggc
     4081 aggcccacga aaagtcaaga gatttttgga ggaaaatggg taatcccacg cataccattc
     4141 ctttctagac gcgctagacg ctcgccaagg gggatgcacg taaccaggca tcgtatctca
     4201 tgataacgga tttacgaatc ccgacgaaca atctaacaac cttccagaga ggaatatatg
     4261 cggaacaaca cacatcactc ggaatcggct ttcaagcctt tcaacgggtt ctggttcggg
     4321 ctggcagcgc tggcgctcat cgttatcctt gcagcatgtg gcggcacaag caccggctca
     4381 ccccctgctt ctggtggcac aacaccgacc gcgacaagcg gtggtggcag tacacccacc
     4441 accgcgccat ccggcaacac agtagccgtc acgattacga ccgacagcag cggctctttc
     4501 gcgttcagcc ccgcggccgt taccatcaag gcaggcacca ccgttgtctg gacgaataca
     4561 accggcgcgc cgcataccgt caccagcgac gacggcaaaa ccttcgactc cggcatagcc
     4621 aatcccatac cagcttcagg cggcacgttc aaatttacct tcaacaccac cggcagcttc
     4681 tcctaccatt gtcagattca cccctacatg aaggcgacca tcgtggtaca gtaatgctga
     4741 tcttgccacc atgccaactc tatgctagac ttctgccagg gttcaacgac ttctgtaggg
     4801 gcgggggcag ggcggtgaag ggaggggacg cttgcgtcgc ccgcgta
//
LOCUS       DASZAK010000828         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_270146, whole genome shotgun sequence.
ACCESSION   DASZAK010000828 DASZAK010000000
VERSION     DASZAK010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270146"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VGT44_14590"
     CDS             complement(<1..552)
                     /locus_tag="VGT44_14590"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2582081.1"
                     /translation="MDKCPTCGADTRPGDNFCLNCGNRLTVSAPPAQPGMGVSGTDTA
                     MATLADDGGWAPATPPTAPATPWNDADLLTVASTSAESPTLRAENPVPSMQAVMNKIE
                     NPARLILRSDSGEVLQEYVLDKAEMSIGRAPNSDILLSKDKLTSRRHATVLYANGTYS
                     IQDERSANGTFVNSQQLDQVTPRQ"
     gene            complement(736..1554)
                     /locus_tag="VGT44_14595"
     CDS             complement(736..1554)
                     /locus_tag="VGT44_14595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582082.1"
                     /translation="MSFARLSSPGVDLNSHTTTILRFLIWRKKQGAYAGLAVFLPLLL
                     MLLVSCSNPFGGSNGGGLDQASPAPGTTAPAQALTSLNWCDKPSMLFRDEGARPSPTA
                     TATSATTATPTVSATTPGASPTITVTPTVGSGTPTTITSWQDVKSGLGFTVYLPTALP
                     GGTCLVSAQATIHDPTLGGNFLIGYLLPDHTSLTISEAPLISQNTTFQCNASASNSTQ
                     KSSAGSGTPTPLPVQLCSGTKDTTNIVLSAQRSVVYLQQLFTALQPNIAWIPAS"
     gene            1845..>2773
                     /locus_tag="VGT44_14600"
     CDS             1845..>2773
                     /locus_tag="VGT44_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEV2582083.1"
                     /translation="MNAKQIWQTTIERLQTRVQPAVFRTWFQGTAALSFQENILIVRV
                     PTTFAKAHLEGRFVELIRSILADVTGSQVEVRFVVAKETLIGNETVVEAPPPTTSYKR
                     SYRIPRSRLESLQEEKSRLDAAFVANSASPTPTRPVAHRQGSAEEVYMGQDEPTTPPP
                     AMADSAPVSYTIRSSRQEAQSQVRNAQPGMFPLHSEGMLNPRYTFSTFIMGKSNQLAH
                     AASLAVAESPGQVYNPLFFYGGVGLGKTHLLHAIGQVGEASGLNVLYVTSEKFTNEII
                     NAIRFQKTEEFRAKYRQIDILLVDDIQFIAGKES"
BASE COUNT          624 a          724 c          776 g          649 t
ORIGIN      
        1 ctggcgcggc gtgacctggt cgagttgctg cgagttgacg aaggttccat ttgcgctgcg
       61 ctcatcctga atcgaatacg tcccattcgc atacagaacg gtggcgtgtc gtcgcgaagt
      121 cagtttgtcc ttcgacaaca gaatgtcgct gttgggcgcg cgaccaatac tcatctctgc
      181 cttgtccagc acgtattcct gtaagacctc gccgctgtct gagcgaagaa tgagacgagc
      241 cggattctcg attttgttca tcacggcctg catggatggc acgggattct cggcgcgcaa
      301 ggtaggcgac tctgctgatg tgctggcgac ggtcagcagg tctgcatcat tccagggggt
      361 agctggcgcg gttggcggag tggctggcgc ccaccctcca tcatcggcca acgtcgccat
      421 ggcggtatca gtaccggata ctcccatacc aggctgagca gggggagccg agacggtcag
      481 acgattgccg cagttcaaac agaaattgtc tcctggccgg gtatcagcgc cgcaggttgg
      541 gcatttgtcc atagtagatg ttcctcccaa tggtaaaaga tcgaacgggc atggagtgaa
      601 cgcaaacctt gcccattata tcagaccgca cgctggggcg caattttttc tctaaaagaa
      661 ccaccagagg cgaccgatgg tacaacgaat ctagttgcgg atcatcgagt agttgataaa
      721 aggaataatc ctcctttatg atgcaggtat ccaggctata ttgggttgta acgcggtaaa
      781 aagttgttgt aagtatacga cacttctctg cgccgatagc acaatattgg ttgtgtcttt
      841 tgttcctgag cagagctgca ccggtagcgg tgttggcgtt cctgagcctg ccgacgactt
      901 ttgtgtgctg ttcgaagcag aagcattgca ttgaaaggtc gtattctgcg agatcaaggg
      961 tgcttctgag atggtcagcg aggtgtgatc cggcagtaag tagccgatca gaaaattgcc
     1021 gcccagggtc gggtcatgga tagtcgcctg cgcgctgacc aggcaggtgc cgcctggaag
     1081 cgcggtcggc agatagaccg tgaaaccgag tcctgatttc acgtcctgcc agctggtaat
     1141 cgtcgttgga gtacccgacc cgacggtcgg cgtcaccgtg atcgttggtg atgctccagg
     1201 cgttgtcgcg ctgacggtcg gcgtcgctgt ggtggcgctc gttgcggtag ccgttggtga
     1261 tggccgcgcc ccttcgtcgc ggaacagcat cgatggctta tcgcaccagt tcaggctggt
     1321 cagcgcctgt gctggtgcgg ttgtgcctgg tgcgggtgac gcctggtcca atcctccacc
     1381 attgctacct ccaaaaggat ttgagcaaga gacaagcagc ataagcaaga gcggcaagaa
     1441 aactgccagc cctgcatacg ctccttgctt ttttctccat atcaaaaagc gcaaaatcgt
     1501 tgttgtgtgg ctgttcaaat cgactcccgg agacgataaa cgagcaaaag acatagagac
     1561 tcctctttta acatagaacg cccatctacc ccttttgtga attcgagtag taagaatgcc
     1621 tcattatttc tgctattcgt gtttgccctc ttttttgtct actatatact aacaaggaag
     1681 ggaatcctat gagctagttc tcaattctta tcctataaag aggggcttta cagcgttttg
     1741 atttagtgtt accattacgc actgcaactc aaaaatcaag ctctacacgt acaaacgcat
     1801 gaacatgctc attggtatag gaatcaacca agaggtaaca tggtgtgaat gcaaagcaga
     1861 tctggcaaac aactatagaa cgcttacaaa cgcgcgttca accggcggtc ttccgaacct
     1921 ggtttcaagg aacggctgcg ctctccttcc aggagaatat attgattgtg cgagtgccta
     1981 cgacgtttgc aaaagcccac ctggaagggc gatttgtgga attgatccgc tcaattctgg
     2041 cggatgtgac ggggagccag gtggaggtgc gttttgtcgt ggcgaaagag accttgatcg
     2101 gcaatgagac tgtggtagag gcaccgccgc cgaccacgag ctataaacgc tcctatcgta
     2161 ttccgcgcag caggttagag tcgctccagg aagaaaaatc gcggcttgat gctgcatttg
     2221 ttgccaacag cgccagtccg actccgaccc ggcctgtggc ccatcgccag ggctctgctg
     2281 aagaggtata tatgggtcag gatgagccaa caactcctcc acccgcaatg gctgattccg
     2341 cgcctgtctc gtatacaatc cggtccagcc ggcaggaggc gcagagccag gtgaggaacg
     2401 cgcagccggg tatgttcccc ctgcactcgg agggcatgtt aaacccgcgt tatacgttca
     2461 gcaccttcat catgggcaag tcgaaccagc tcgcccacgc ggcatcgctg gccgtcgcgg
     2521 aaagtcccgg ccaggtctac aatccgctct tcttctatgg cggcgtcggc ctgggcaaga
     2581 cccacctgct gcacgccatc ggacaggtgg gtgaggcgtc tggcttgaac gtactctacg
     2641 ttacctcgga aaaatttacg aacgagatta tcaacgctat ccgcttccag aagaccgagg
     2701 agtttcgcgc caaatatcgc cagatcgata ttttgctggt cgatgatatc cagtttatcg
     2761 cgggcaagga atc
//
LOCUS       DASZAK010000829         1620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_696048, whole genome shotgun sequence.
ACCESSION   DASZAK010000829 DASZAK010000000
VERSION     DASZAK010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696048"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VGT44_14605"
     CDS             complement(<1..733)
                     /locus_tag="VGT44_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV2582084.1"
                     /translation="MRRTKIVCTIGPSTSSEERLEELMRAGMNVARLNFSHGTHDEHA
                     AVIERVRAISARIECAVAILQDLQGPKIRTGALENGQPVRLVDGATLTITTRPIAGNA
                     QIVSTTYTQLPLDVKPGDRILLDDGLMELCVVDANENEVHCQVVHGGTLKEHKGINLL
                     GVAVSAPALTEKDRDDLRFGLLHRVDYVALSFVRKPEDILEAKAFIRAVLSEQGTSRP
                     AKQAYLPPPLIAKLEKPEAVARLDEI"
     gene            complement(750..>1620)
                     /locus_tag="VGT44_14610"
     CDS             complement(750..>1620)
                     /locus_tag="VGT44_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEV2582085.1"
                     /translation="ESYRSYLFAIAYRMVGCASDAEDIVQDAYLRYRQARSGEIHTLK
                     AYLATLVTRLCIDYLKSARVQREQYIGPWLPEPLLTSGQETLPFETAAQHESISLAFL
                     VLLETLSPTERAVFLLHDIFDFDFQEIAAMLGKSPVNCRQLCHRARASIAERKHRFEP
                     SRELHQRLINRFLLAVQEGDIEGLQQILAQDVVNFGDGGGKALSALRPVVGIDAVMRL
                     WIALAHKPPADLKLTFEEVNGSPAVMLWTGETLYNVLSFEVVDGKIQAIRGILNPDKL
                     TFIARQLRAHPQA"
BASE COUNT          304 a          492 c          485 g          339 t
ORIGIN      
        1 ggatctcatc gagtcgcgca accgcctccg gcttttccag cttggcgatc agtggtggcg
       61 gcagatatgc ctgcttcgct ggcctgctcg ttccctgttc cgacaggaca gcgcgtataa
      121 atgctttcgc ctccaaaata tcctcaggtt tgcgcacgaa gctgagcgcg acataatcta
      181 ccctgtgcag caggccgaaa cgcaggtcgt cgcggtcctt ctccgtcagg gcgggcgcgc
      241 tgaccgccac tcctagcagg ttgatgcctt tgtgttcctt gagggtgcct ccatgcacaa
      301 cctggcaatg cacctcgttc tcgttcgcat ccaccacgca cagttccatc agcccatcgt
      361 ccagcaagat acggtcgccc ggcttcacat ccagtggcag ctgtgtatag gtggtcgaga
      421 cgatctgtgc gttgcccgcg atggggcgcg tggtaatcgt cagagtcgcg ccatccacca
      481 gcctgaccgg ttggccattt tccagcgcgc cggtgcgtat cttcggccct tgcaggtcct
      541 gcaggatggc gacggcgcac tctatgcgcg ccgaaatcgc gcgcacacgc tctatgacgg
      601 cggcgtgttc gtcgtgcgtt ccgtgcgaaa agtttaagcg cgctacgttc ataccagcgc
      661 gcatcagctc ttcgagccgc tcttcgctgc tggtggacgg gccgatcgta caaacaatct
      721 tggttcgccg catttgagac tcctctaccc tatgcttgtg gatgcgcccg caactggcgc
      781 gcgataaatg tcagcttgtc tgggttcaag ataccacgaa tggcctggat tttgccatca
      841 accacctcga agctcagcac gttgtagagc gtctcgcctg tccataacat tacggcgggg
      901 ctgccattca cctcttcaaa ggtcagcttg aggtcggcgg gcggcttgtg cgccagcgct
      961 atccagaggc gcatcaccgc gtcgatgccc accaccggtc ggagagcaga gagggctttg
     1021 ccgccgccat cgccaaaatt gaccacatct tgcgccagta tctgttgcag cccctcgata
     1081 tcgccctcct ggacggcgag caagaagcga ttgatcaggc gctggtgcag ttctctcgac
     1141 ggctcaaagc gatgtttgcg ctcagcgatg gaagccctgg cacgatggca aagttggcga
     1201 caattgacgg gacttttgcc aagcatggcc gcgatttcct ggaaatcaaa gtcgaagatg
     1261 tcgtgcagga gaaaaacggc gcgttccgtg ggcgagagcg tctcgagcag gacgagaaag
     1321 gcgagcgaga tggattcgtg ctgcgcggcg gtctcaaacg gcagcgtctc ctggccggag
     1381 gtgagcagcg gctccggcag ccaggggcca atgtactgct cgcgctggac gcgcgccgat
     1441 ttgagataat cgatgcagag gcgcgtgacg agcgtcgcca ggtacgcttt cagcgtgtgg
     1501 atttcgccgc tgcgcgcctg ccgatagcgc agataggcat cctgcacgat atcctccgca
     1561 tcgctcgcgc agcctaccat gcgataggcg atggcgaaca ggtacgagcg gtagctttca
//
LOCUS       DASZAK010000830         1529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_304566, whole genome shotgun sequence.
ACCESSION   DASZAK010000830 DASZAK010000000
VERSION     DASZAK010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304566"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1419)
                     /locus_tag="VGT44_14615"
     CDS             complement(67..1419)
                     /locus_tag="VGT44_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582086.1"
                     /translation="MSLREAIQRYNDLLTDNLAAASEAQLESQQRQRGLMFSNRPLCT
                     VLRPRFMTPEQYRFLQARMTMLLRAFDKAYHAAMEQEHVRKQFGLLDWEEELIKYDPG
                     FRDASPTSRVDTFFVTERGGLRLTEYNAETPASPAYNDALAEVFYGLPVMREYLRQYE
                     VRPLPSRHGVMHALIDAYQQWAGHREAPRIAILDWREVPSYSEFVLFAEYFRSQGLEC
                     IIADPREVEYRDGKLMAGDFHITLIYKRVLISELITRGGLNHPVVRAVLDGAVCMVNP
                     FRCKILHKKASLAVLSDERNADLFNVDELAAIDAHIPWTYRVEDRRARYRGQSIDLLP
                     YIFEHRENFVLKPNDEYGGKGIVLGWEVSAEEWGNAIQGALQEPYIVQERVAIPSESY
                     PSMVDGHVTFIDRMLDTNPLIFYGSYMDGCLSRLSTEALLNVSAGTGSAVATFVVEKR
                     "
BASE COUNT          292 a          445 c          483 g          309 t
ORIGIN      
        1 caggccgatc aatcggcggt gtgcgcggta aaccggcacc tacagttatt cgcagtgttc
       61 gacaaattag cgcttctcga cgacaaacgt tgcaacagcc gagccggtgc cggcggagac
      121 attcaacaac gcctcggttg aaaggcgcga gagacagcca tccatgtagc tgccgtagaa
      181 gatcaaaggg ttggtgtcga gcatccggtc aatgaaggtc acatgaccat cgaccatact
      241 gggatacgac tcgctgggga tagcaacgcg ctcctgcacg atatatggct cttgcagcgc
      301 gccctgaatg gcgttgcccc attcctcggc gctgacctcc cagcccagca cgatgccttt
      361 gccgccatat tcgtcgttgg gcttcaggac gaaattttcg cgatgctcga aaatgtaggg
      421 cagcagatcg atggattggc cacgatagcg cgcgcggcgg tcttcgacgc gatacgtcca
      481 gggaatatgc gcatcgatag cggccagttc atcgacgttg aacaggtcgg cgttacgctc
      541 gtcgctcagc acggccaggc tggccttttt gtgcaggatt ttgcagcgga acgggttgac
      601 catgcagacc gcgccatcca gcacggcgcg cacgacgggg tggttgagtc cgccgcgcgt
      661 aatgagttcg ctgatcagca cgcgcttgta gatgagcgtg atgtggaaat ccccggccat
      721 cagtttgccg tcgcggtatt cgacttcgcg tgggtcggcg atgatgcatt ccagcccctg
      781 cgagcggaaa tattcggcga acagcacgaa ctcgctgtaa ctggggactt cgcgccagtc
      841 gaggatggcg atgcgcggcg cttcgcgatg acccgcccat tgctgatagg cgtcgatcag
      901 cgcgtgcatg acaccgtgcc gcgagggcag gggccgcact tcgtactgac gcaggtattc
      961 gcgcatgacc ggcaggccgt agaacacctc cgccagcgcg tcgttatagg cgggactggc
     1021 gggcgtttcc gcgttatatt cggtcaggcg caggccgccg cgctcggtga cgaagaaggt
     1081 atcgacgcgc gaggttgggc tggcatcgcg aaagcccgga tcgtatttga tcagttcctc
     1141 ttcccagtcg agcagcccaa attgcttgcg cacgtgctcc tgctccatcg cggcgtggta
     1201 cgccttgtcg aaggcgcgca gcagcatcgt catgcgcgcc tggagaaagc gatactgctc
     1261 cggcgtcata aagcgtggcc gcagcaccgt acacagcggg cggttgctga acatgaggcc
     1321 gcgctggcgc tgctgcgact ccagttgcgc ttctgacgcg gcagccaggt tgtccgtcag
     1381 gagatcgtta tagcgttgaa tggcttcacg cagtgacatg ctcgcacctc caggtgatct
     1441 ctattgttct gtcaaggtta atgtggcgtg taggggcgca attcattgcg ccctcgttcc
     1501 gggtcacgcg ccgccacctg ggcgcaatg
//
LOCUS       DASZAK010000831         6437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_412608, whole genome shotgun sequence.
ACCESSION   DASZAK010000831 DASZAK010000000
VERSION     DASZAK010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412608"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VGT44_14620"
     CDS             <1..616
                     /locus_tag="VGT44_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582087.1"
                     /translation="DLAYIQNLANRNGYTFYLEPGPAPGTSVAYWGPKIMAGVPQSAL
                     NINMDADTNVESLSFSLDGRAKEQDIVFVYDPITHKIPIPVPLPNINLLHPPLGVRPS
                     PPLKIKLLKGTSKLNQIEAILLGLSETANSADSITGNGQLDVLRYGHVLKARGLVGVR
                     GVGLAYDGLYYVNSVTHNIKRGEYKQSFTLSRDGLISLTPRVLP"
     gene            613..1140
                     /locus_tag="VGT44_14625"
     CDS             613..1140
                     /locus_tag="VGT44_14625"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEV2582088.1"
                     /translation="MTDTKEISYYGKYRGVVFNNIDPENRGRIQAVVPDITSLIPSTW
                     AEACVPLAGGPPPMGIYVVPPIGANVWIEFEQGNPNYPVWTGCLWGFQAEVPSLALAG
                     VPGDPNIVLQTTGQNTLMLSDLPGPTGGIMLKSTSGAMILVNDIGITISNGKGATITL
                     IGNTITMNQGALTII"
     gene            1158..1493
                     /locus_tag="VGT44_14630"
     CDS             1158..1493
                     /locus_tag="VGT44_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR-like protein"
                     /protein_id="HEV2582089.1"
                     /translation="MPGFLLHVGATIICPHAGQVTAISTNTRVLVSGQPVVTFADTFT
                     VAACVFTVPGPKPQPCVKVQWITPATRVFVNGQPALLQTSTGICQSVEQIPQGPPSPV
                     AVQARVGGM"
     gene            1497..1853
                     /locus_tag="VGT44_14635"
     CDS             1497..1853
                     /locus_tag="VGT44_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEV2582090.1"
                     /translation="MNIAFPFHFDGRGRTASVGNDQHIQDMIEQLLFTNPGERVNRPD
                     FGSGLQQLVFAPNSPELAAALQFTLQAALQRWLSDLIELQSLEVTSDDSELSISIQYI
                     VRRTNERQVAQFTQAV"
     gene            1868..4582
                     /locus_tag="VGT44_14640"
     CDS             1868..4582
                     /locus_tag="VGT44_14640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEV2582091.1"
                     /translation="MSCKNKHKRRQRVRDQNAERKVYGLDYIEVSPDQKTLTVYFLGS
                     APKDIKKENVRIDGGRRIRNIQVTGVELHHESKEERDDYMEVKVDQPGDFSTYTLSMV
                     TTTDAQGHSVEVPFTGFDDRYGQLDFSFKVDCHNDLDCLPVDTCPPPAFDEPEINYLA
                     KDYASFRQLILDRLALIMPAWKETHVPDLGIALTEILAYVGDYLSYYQDAVATEAYLD
                     TARQRISVRRHVHLVDYIMHEGCNARAWVIVKTSDDVINPPLNPKEFYFITGYNNALQ
                     VSSTVLRERDLRNIPAGSFDVFEPLVPPTTPAHQTPHKKHHHHQAQTPAQPAPAIQLY
                     KAHNLIEFYTWGEEDCCLPRGATSATLRDHWEPQQDQPVQSKQQKQQAHGEPGEDEPE
                     DEPSPPAPQPERKRRLHLQVGDVLIFEEVLGPKTGNPADTDHKHRCAVRLTNVTPIID
                     ELYDRPVVDIAWAVEDALPFPLCLSAIGQAPKCKLIENISVARGNVFLVDHGLTGTED
                     IPGSVPAQPAVPQCEDEGCGDEASKVPGRFRPQLQNAPLTFSQPLAVGNTPAIRLLIQ
                     DPRQAVPQVLDMVSTPDPTSHAAPQHWHVRRDLLESSAQDYDFVVEMDNDSYAHLRFG
                     DGELGHAPEVGSTFSATYRVGNGPTGNVGAGAISHIVFRDDATSVPDTVTLESRNPLP
                     AVGGVAPEPLEEVKLFAPHAFRSDLQRAITADDYAQLAERNPKVQRAAATLRWNGSWY
                     EVLVAIDPLGSEEADPALLRAITRYLRKYQRIGHDLLVVPANYVSLDIEMNVCVLPHY
                     LRGHVKAALLDLFSNRVLPGGKLGFFHPDNLTFGVSIAASKLVAAAQAVTGVQSVSVT
                     RLQRLFEGPKHELPNGILPIGPLEIARLDNDPSFPENGKLTLVMGGGR"
     gene            4627..>6437
                     /locus_tag="VGT44_14645"
     CDS             4627..>6437
                     /locus_tag="VGT44_14645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582092.1"
                     /translation="MSIANRPGLDALAYRVGTQASFLETMKARLSNLGITLDALEAAA
                     TGAGQPGTQLYPLQGLSTRAENDPAIAFLDAWATVADVLTFYQERIVNEGYLRTATER
                     RSILELARLVGYDLRPGVSASVFLAYTVDTGTTTIIPVGSRAQSVPDPGEQPQPFETS
                     ADLEARGKWNDLQPRLTRPTFIVKENADIVDTVDFQGTSTNLKPNDPLLLVFDDNSGV
                     PAGQILRFVHDVEDQAKDNRTKVTLQVVLTDPSFIRAIQQTVARYLDLPSFCVSATDP
                     ITGNVEVILADFFDPQKVFSAFIADVQSLQDQLTQAQKDGNAVIVIWLSALIADLEKI
                     LNWVPFGIIDSNNPGLQGLFAQIQEKIKKDKQVLLPTTDVASTQIKLGSLLVPLLKPA
                     SLQPANTLRLDRSVKQAFSFSSDIHPQLLTVLNPALGTTLYTAVSNAQITQASALESA
                     QALRVKSAPFGHNAPLKPVFDDKGKLIGQEEWPLSNSVKIGITLSFLELQLSVAKAAS
                     ATPPPLPSSLGGSTIIGIEVSLQQGGVTQSTSVPLQAGEQTISFEAPFANQGISITIN
                     PNATTPQDIDSIVFNFTQLGQKIQITPQNQGAAIGVQV"
BASE COUNT         1522 a         1853 c         1717 g         1345 t
ORIGIN      
        1 cgacctcgcc tatattcaaa atctggcaaa tagaaacggc tatacgtttt acctggaacc
       61 aggaccagca ccaggtacca gtgtcgccta ctggggcccg aagatcatgg caggtgtgcc
      121 tcaatcggca ttgaatatca acatggatgc cgacacgaac gtcgaatcct tgagttttag
      181 tctggatggg cgcgcaaaag agcaggacat agtcttcgtc tatgacccaa ttacccacaa
      241 aatacctatc cctgtgcctt taccaaatat aaatttgttg catccgccgt taggtgtgag
      301 gccgtcgccg ccgttgaaaa taaagctgct gaagggcaca tcaaaactga atcaaatcga
      361 agccatcctg cttggacttt cagagaccgc caattcagcc gactcgatca ccggcaacgg
      421 gcaactggat gtcttgcgct acgggcatgt gctgaaggcg cgaggactcg taggtgtgcg
      481 cggcgtggga ctggcctacg atggcctcta ctacgtcaac agcgtaacac acaacatcaa
      541 gcgcggcgag tataagcaga gctttacctt atcaagagac ggactgatct cattaacgcc
      601 gagggtattg ccatgactga tacaaaagaa atctcctact atggaaagta tcgtggggtt
      661 gtcttcaaca atatcgatcc tgagaatagg ggacgcatac aggcggtcgt gcctgatatc
      721 accagcctca ttccttctac atgggccgag gcttgcgtcc ctcttgcagg tggaccgccg
      781 ccgatgggca tctacgtggt tccgcccatt ggggccaatg tgtggataga atttgagcaa
      841 gggaacccca attacccggt ctggacgggc tgtttgtggg gctttcaagc ggaagttccg
      901 tccctggcac tggccggtgt gccgggtgat ccgaatatcg tgctgcagac gacgggacaa
      961 aacacactta tgctcagcga cctcccagga cctacaggcg gcatcatgct caaaagcacg
     1021 tcgggagcca tgattcttgt gaacgatatt ggcattacaa tttcaaatgg caaaggggca
     1081 acgatcacgc tgatcggcaa tacgatcacc atgaatcaag gcgctttaac cattatctaa
     1141 caagtatgga ggtacctatg ccagggtttt tactacacgt cggagcaacg atcatctgcc
     1201 cgcatgccgg gcaggtgacg gccatctcca ccaatacacg cgttttggtg agcggtcagc
     1261 cggtggtgac ttttgccgat acatttacgg ttgctgcttg tgtgtttacc gtgccaggcc
     1321 cgaagccgca gccgtgtgtc aaagtccagt ggattacgcc tgctacgcgg gtattcgtca
     1381 atggtcagcc agcgttgctg caaacgagca cgggcatctg ccagagtgta gagcaaatcc
     1441 cgcaagggcc accaagcccg gtcgctgtgc aggcccgcgt aggaggtatg tgagcaatga
     1501 atatcgcctt tcccttccac ttcgatggtc gcggtcgcac cgcctccgtg ggcaacgatc
     1561 aacatattca ggacatgatc gagcaactgc tcttcaccaa tccaggtgaa cgcgtcaatc
     1621 gtcccgactt cggcagcggc ctgcagcaac tggtctttgc ccccaacagc ccagaactgg
     1681 cggcagcgct ccaattcacg ctgcaggccg cgctgcaacg ctggctcagc gacctgattg
     1741 aactgcaatc tctggaggtg accagcgacg attccgaact ctccatttct attcaatata
     1801 ttgtgcgtcg tacgaatgag cgccaggtag cgcaattcac gcaggcggtg taatagtgac
     1861 aacagacatg tcttgtaaaa ataagcataa acggcggcag cgcgtacgcg accagaacgc
     1921 tgagcgcaag gtatacggcc tggactacat agaggtcagc cccgaccaga aaaccctgac
     1981 agtctacttc ctgggcagtg ccccaaagga tatcaagaag gagaatgtgc gcatcgatgg
     2041 agggaggcgc atacgcaata tccaggtgac aggcgtagaa ctgcatcacg agagcaaaga
     2101 agaacgagac gattacatgg aggtgaaggt agatcagccg ggcgatttct ccacctacac
     2161 actgagcatg gtcacgacga cagatgcgca gggtcactca gttgaggtgc cgtttacagg
     2221 ctttgatgat cgctatggtc agttggactt ctcctttaaa gtggactgtc acaacgatct
     2281 cgattgtctg cccgtcgaca cctgcccgcc gcccgctttc gacgagccgg agatcaacta
     2341 cctagcgaaa gactatgcca gcttccgcca gctcatcctt gatcgcctgg cgctgattat
     2401 gccagcgtgg aaggagactc acgtccccga cctgggtatt gcgctcacag aaatcctggc
     2461 ctacgtcggc gactacctga gctattatca agacgccgtc gccaccgagg cttacctgga
     2521 caccgctcgc cagcgtatct ccgtgcgtcg ccatgtgcac ctggttgact atatcatgca
     2581 cgaaggctgc aatgcgcgtg cctgggtcat cgtgaagacg agcgacgacg tcatcaaccc
     2641 gccgctgaac ccaaaggagt tctactttat cacaggctat aacaatgcct tgcaggtaag
     2701 tagtacggtg ctgcgagagc gcgacctgcg caacatccct gcaggttcct tcgacgtgtt
     2761 tgagccgctc gtgcctccga caacgcctgc gcatcaaacg ccgcacaaaa agcatcacca
     2821 ccatcaagca cagacaccag cacaaccggc accagcaatt caactctaca aggcgcacaa
     2881 cctgatcgag ttctatacct ggggcgagga ggattgctgc ctgccgcgtg gagccaccag
     2941 cgcgacgttg cgggatcact gggagcccca gcaagatcaa ccagtgcaaa gcaagcagca
     3001 gaaacagcaa gcacatggcg agccgggaga agatgagccg gaggatgaac catctcctcc
     3061 tgcgcctcaa cccgaacgaa agcgcagatt gcatctgcaa gtgggtgacg tactcatttt
     3121 tgaagaggta cttggcccca aaactggcaa tcccgccgac accgatcaca aacaccgctg
     3181 tgccgtgcgc ctgacgaacg tgacgccgat tatagatgaa ttatatgacc gaccggtggt
     3241 tgacatagcg tgggccgtgg aagatgcctt gcctttcccg ctctgtctct cggctatcgg
     3301 acaggcacca aagtgcaagc taatcgaaaa tatcagcgtg gctcgtggca atgtcttcct
     3361 cgtcgatcac ggcctgacag gcacagaaga tattcctggc agcgtccctg cacaaccggc
     3421 agtgcctcaa tgcgaagatg aaggttgtgg agatgaagcc tcgaaagtgc ccgggcgttt
     3481 tcgtccgcag ttgcagaacg cgcccttgac cttcagccag ccattggctg tcggcaatac
     3541 tccggccata cgtctgctca tacaggaccc gcgccaggcg gtgccccagg tactcgacat
     3601 ggtgagcaca ccggacccga ccagccatgc cgctccccag cactggcatg tacggcgcga
     3661 cctgctagaa agttcggccc aggactacga tttcgtcgtt gaaatggata atgacagcta
     3721 cgcacacctg cgctttggcg atggggaact gggccacgca ccggaggttg gttccacctt
     3781 ctcggctacc tatcgtgtag gcaatgggcc aacgggcaat gttggcgccg gtgcgatctc
     3841 tcatatcgtc ttccgtgacg acgctaccag cgtaccggat actgtaacgc tggagtcacg
     3901 caatcccttg cccgccgtgg gcggtgttgc tcccgaaccg cttgaagagg tcaagttgtt
     3961 tgcgccacat gccttccgta gcgacctgca gcgtgctatc accgccgatg actacgcaca
     4021 gctcgccgaa cgcaatccca aagtgcagcg tgcagccgcg acgctgcgtt ggaatggcag
     4081 ttggtacgag gtcctcgtgg cgattgatcc actggggagc gaggaggcgg acccggcact
     4141 gttgcgcgca ataacccgct acctgcggaa ataccagcgt attggtcatg atctgctcgt
     4201 ggtgcctgca aactatgtct cgctcgacat cgagatgaac gtctgtgtgc taccgcacta
     4261 cctgcgtggg cacgttaagg ccgccctgct cgatctgttt agtaatcgcg tcctgccggg
     4321 cgggaaactc ggcttcttcc atcccgataa cctgaccttt ggcgtgagca ttgctgccag
     4381 taagctggta gctgcggccc aggcagtgac cggggtacag agcgtctctg tcaccagact
     4441 gcagcgcctc ttcgaggggc cgaaacacga actgccgaac ggcatcttgc ccattggacc
     4501 attggaaatt gctcgcctgg ataacgaccc cagtttccct gaaaacggga agctcaccct
     4561 cgtcatggga ggtggacgat gaacgaaact tgtggctgct gcgaaggtac gcaggtactc
     4621 accccgatgt caatagccaa ccgacctggc ctggatgcac ttgcttatcg cgtcggtacg
     4681 caggccagct ttctggagac gatgaaggct cgtctctcaa acctgggcat tacgctcgat
     4741 gcgcttgagg ctgctgcgac cggtgctggt caaccgggta cgcagctcta tcctctacaa
     4801 ggattgtcca cgcgtgccga gaacgacccg gccatcgcct tcctggacgc ctgggccacc
     4861 gtggccgatg tactcacgtt ttaccaggag cgcattgtaa acgagggcta cttgcgcacg
     4921 gcgaccgagc gacgctccat cctggaactg gccaggctcg tgggatacga cctgcgcccc
     4981 ggtgtctctg ctagcgtctt cctggcctac accgtggata ctggcacaac gacaatcatt
     5041 ccagtaggaa gtcgtgccca aagcgttccc gaccccggcg aacaaccgca accctttgaa
     5101 acctctgctg acctggaggc gcgtggcaaa tggaacgacc tgcagccccg cctgactcgc
     5161 ccgacgttca tcgtcaagga gaacgcagat attgttgata cggtagactt ccagggcact
     5221 tcgacgaacc tgaagcccaa cgacccgctc ctgctcgttt ttgatgacaa tagcggagtt
     5281 ccagcagggc aaattttgcg ctttgtgcat gatgttgaag accaggcgaa ggataaccgc
     5341 accaaagtca cactgcaagt cgtgcttacc gacccttcat tcatcagggc catccagcaa
     5401 acggtggcgc gttatctcga tctgccctcg ttttgtgtct ctgctacgga ccctataacc
     5461 ggcaacgtag aagtgattct tgctgacttt ttcgatccgc aaaaggtctt cagtgcattt
     5521 attgctgatg tacaaagttt gcaagaccag ctcacccagg cccaaaagga tggaaatgcc
     5581 gtcatagtga tctggctcag cgctttgata gccgacctgg agaaaatcct gaactgggtg
     5641 ccatttggca ttattgacag taataaccct ggcttgcaag ggttgtttgc gcaaatacaa
     5701 gaaaaaatca aaaaggataa acaagtactg ctgcccacta ctgatgttgc cagcacgcag
     5761 atcaagttag gctccctgct cgtcccactg ctgaagcctg cttctttgca accggccaat
     5821 accctgcgcc tcgaccgcag tgtcaaacag gcattttcct tctcgtccga cattcacccg
     5881 cagttgctca cggtcctcaa tccagccctg ggtacgacac tctataccgc tgtgagcaat
     5941 gcgcagatca cgcaggcctc ggctctggaa agtgcgcagg ccctgcgggt caaatcggct
     6001 ccctttgggc acaatgcacc gctcaagcct gtgtttgatg ataaagggaa actgattggc
     6061 caggaagaat ggcctctgag taatagcgtg aaaatcggca tcacactcag cttcctcgaa
     6121 cttcagctta gcgtggcaaa agctgccagc gcaacacctc cgcccttgcc ttcctccttg
     6181 ggagggagta cgatcatagg aatagaggtg tcactccaac aaggtggagt gacacaaagt
     6241 acatcggtac ctttacaggc gggagagcag actatctcgt ttgaggcacc atttgccaac
     6301 cagggcatca gtataacgat caaccctaat gcgactactc ctcaggatat cgatagcata
     6361 gtttttaatt tcacacaact gggtcagaaa attcagataa cacctcaaaa ccagggtgct
     6421 gctataggcg tacaggt
//
LOCUS       DASZAK010000832         6198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_377342, whole genome shotgun sequence.
ACCESSION   DASZAK010000832 DASZAK010000000
VERSION     DASZAK010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377342"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VGT44_14650"
     CDS             <1..777
                     /locus_tag="VGT44_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2582093.1"
                     /translation="SPQPLPEHPGGYGLSFEHVTFTYQSGGREVLSDINFTVRPGSRV
                     AIVGPSGAGKSTLVRLALRFWDPQSGAICLDGQDIRRFSLRDLRDQIAVVAQDTYIFN
                     DTLRANLLLARPKASEAEIEEALEQAQLTDFVRQLPQGIKTRVGEQGLQLSGGERQRL
                     AIARAFLKNAPILILDEATANLDPLIERALLDTLNELSRGHTTLQMTHRLLGMEHMDE
                     ILVLHRGCLVERGTHDELLALGKLYRQLFDIQEGILAFYE"
     gene            919..2298
                     /locus_tag="VGT44_14655"
     CDS             919..2298
                     /locus_tag="VGT44_14655"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEV2582094.1"
                     /translation="MDTSTIFSRIQFAFTIGYHYLFPQFTMGLALLLVILKILYLVRK
                     DERYNTAVHFWGKIFAMTFVLGVVTGIPMEFQFGTNWALFSTYAGGIIAQTLAMEGAF
                     AFFLESAFLGVFLFGEQPLGQKWHLFATIMVWLGTWASGAFIIASNAWMQHPVGYTLA
                     NGRLNISDWVAVMTNSWILPQYLHTMSGAVITGAFMMTGLGAYYLLARRDIDYGRMFV
                     TLGVIVGLISSVFQLYPSGDMEGAQVTKYQPTKLAAMEGLFQTQSGAGIVILGQPDVP
                     KGTIDNPIIVPNVLSFLTYRRWTAVIQGLDTFPADQRPDSVEMLYYSYHIMVGLGTIF
                     IAIMGLALLLLWRRKLFQSRWMLWLLMLATPFPYIANTAGWFTAELGRQPWIVFGLLR
                     TVHGSSPLLSTGNIIFTLLGFAGIYLLLGLLYVIMVVFQALTGPTPQTTEATSSEEGG
                     DLGLLSEQA"
     gene            2310..3353
                     /gene="cydB"
                     /locus_tag="VGT44_14660"
     CDS             2310..3353
                     /gene="cydB"
                     /locus_tag="VGT44_14660"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEV2582095.1"
                     /translation="MSTLWFILVAFMLTMYVLLDGFDLGAGIIHLVAARTDTERRFIL
                     RAIGPVWDGNEVWLIAAGGTLFFSFPLLYASSFSGFYLPLIIVLWLLIVRGVAIDLRS
                     RFANSVWTSFWDGMFFLGSALLAIFYGAALGNVIRGVSLNAQGYFFEAFWTDFNPFSA
                     NPGILDWYTILTGLLAFTALLTHGANYIATKTEGALNERARRISRVGWLLTVPLAVLA
                     SITTFFVQPQVYTSFTQRPWGIVFPLIAFVGLLGAGFFNLRKRDLPALLSTGTFILGM
                     LSSSAFSLYPLVLPAVNHANSLTIDNASASQYGLAFGLIWWSVGIILAAIYFIITYRR
                     FWGKITPESSQEY"
     gene            3416..4402
                     /locus_tag="VGT44_14665"
     CDS             3416..4402
                     /locus_tag="VGT44_14665"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2582096.1"
                     /translation="MTATYAPGAHIDHYEIIRLLGHGGMNNVYLARDTNCQETVVLKF
                     PNEDLISNVSVFERYKREAEIGSRLDHPHIQRLLNTDEQHSEHYLVMEFIQGKTLREL
                     LDALAPQLLPPHEVARIGIQIGEALTYAHEHGVFHRDIKPENVMILEDGNVKLIDFGV
                     ALLEGARRVTWRGLSGTVGTPDYMSPEQLTGDRGAAGSDVYAVGAMLYEMLCGHVPFE
                     GENVFAVLNQHVTQDPPSILHVNPHVSPALATVIMRSIRRDPEKRYKHMADMLRDLRH
                     LDAVVPVPYHPDKQQYESRSRRILLLCLVTIAICLTIIAIGFLAQFVHNAVH"
     gene            4707..>6198
                     /locus_tag="VGT44_14670"
     CDS             4707..>6198
                     /locus_tag="VGT44_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HEV2582097.1"
                     /translation="MRHVRLNSSNPADPAQPVIDPTYPLYDARFEHDACGTGFLAQIS
                     GEASHALVQTALDALARLTHRGAQDADAETSDGAGLMTQIPRDLLREELRARGISFSD
                     PADLAVGMLFLPSPERSPGAFAHSRQIIEEVLQELDSEWTEGEKLFWRDVPLNVAIPG
                     SRARATMPHIAQIVLLRPSRLALAHYEQTLYFARRLIERGLQAAGIADCYIVSLSHRT
                     VIYKGLLAPNQLNRFYLDLADARYTSAFAVFHQRYSTNTFPSWPLAQPMRALAHNGEI
                     NTLQGNRNWMRACEQDLDSPVWGDRLEQLLPVVQTNGSDSAQLDNILEWLALSGRDLL
                     HSMQMLIPPAWEHDPEMDSAQRAWCEYHAALIDPWDGPAALAFSDGRVVGAALDRNGL
                     RPARYMLTSHGLLVLASEAGVMPTDAHDVVERGRLGPGEMIAVDLERGVLLRDAEIKA
                     ELAQRQPYQQWLEDNMIHLDALPQFSQHSSFDQAEQGMDAGALFSRQ"
BASE COUNT         1195 a         1844 c         1732 g         1427 t
ORIGIN      
        1 tctcctcagc cgcttccaga acaccctggc ggttatggcc tatcgttcga gcacgtcact
       61 ttcacctacc aatccggcgg gcgcgaggtc ctcagcgata tcaatttcac tgtgcgccct
      121 ggcagccgcg tggcgattgt cggccccagc ggcgcgggca aaagcacgct ggtacgcctc
      181 gcgctgcgtt tctgggaccc ccagagtggc gctatttgcc tggacgggca ggacatccgt
      241 cgcttcagcc tgcgcgatct ccgcgaccag atcgctgtcg ttgcccagga tacctatatt
      301 ttcaacgaca cgctgcgcgc caatttgctg ctggcgcgcc ccaaagcgag cgaggcggag
      361 attgaggagg ctcttgaaca ggctcaactg accgatttcg tgcgccagtt gccccagggg
      421 atcaaaacca gagtggggga gcagggcttg cagctctctg gcggcgagcg ccagcggctg
      481 gccattgcgc gcgcattcct gaaaaatgcg cctatcctca tactcgacga agccaccgcc
      541 aaccttgatc ccttgattga gcgagcgctg ctcgatacgc tgaatgaact ttcgcgaggg
      601 cataccaccc tgcaaatgac gcatcgcctg ttagggatgg agcatatgga cgagatcctc
      661 gtgctgcatc gaggctgcct tgttgaacgt ggaacccatg acgagctgct ggcactgggg
      721 aagctctacc ggcaactgtt tgacatacaa gaaggaatac tcgctttcta tgaatgaact
      781 ttaccgacca ggcataaccc tgtcatcctg agcgcagcga aggatctgat gcgccgggga
      841 tcgagatcct tcgctgcgct caggatgaca ttcttgatct tgtttgtgat aaagcataga
      901 tgagctgaaa gaggaactat ggatacatcg acgatctttt cgcgcataca attcgctttt
      961 acgatcggct atcactacct ttttccgcag ttcacgatgg ggctggcgct gctgctggtt
     1021 atcctcaaaa tactctacct ggtgcgcaag gatgaacgct ataatacggc tgtgcatttt
     1081 tggggcaaga ttttcgccat gaccttcgtt ctcggcgtgg tcacgggcat tccgatggaa
     1141 ttccagttcg gcaccaactg ggcgctcttc tccacctatg ccggtggcat cattgcgcag
     1201 acactggcga tggaaggcgc cttcgctttc ttcctggagt cggccttcct gggtgtcttc
     1261 ctcttcggcg agcagccact cgggcaaaaa tggcacctct ttgcgacgat catggtctgg
     1321 cttggcacct gggcatcggg cgcattcatc atcgcctcga atgcctggat gcagcacccg
     1381 gttggctata cgcttgcgaa tgggcgtctg aacatctctg actgggtcgc ggtgatgacc
     1441 aattcctgga ttctgcctca atacctgcac accatgagcg gcgctgtcat caccggtgca
     1501 ttcatgatga ccggcctggg tgcctattac ctgcttgcca ggcgcgacat cgactatggc
     1561 cgcatgttcg tgacgctggg cgtgatcgtg ggcttgattt ccagcgtctt ccaactctac
     1621 cccagcggcg atatggaagg ggcgcaagtg acaaaatacc aacccaccaa gctagccgcg
     1681 atggagggac tgtttcagac gcaaagcggc gcgggtattg tgatcctggg acaacccgat
     1741 gtgcctaaag gcacgatcga caatcctatc attgttccca atgtcttgag cttcctgacc
     1801 taccggcgct ggacagcagt cattcaggga ctcgatacct ttccagccga tcagcgtccc
     1861 gactcagttg aaatgctcta ctatagttat cacatcatgg tcggtctagg cacgatcttc
     1921 attgctatca tgggcctggc gctgcttctg ctctggagac gcaagctgtt ccagtcccgc
     1981 tggatgctgt ggctcctgat gctggcgacg ccgtttccct acatcgcgaa cacggcgggc
     2041 tggttcactg ccgagcttgg ccgccagccc tggatcgtct tcgggttgct gcgcaccgtg
     2101 catggctcgt cccccctgct ttcgacgggg aacatcatct tcacgctgct cggctttgcc
     2161 gggatatatc tcttgctcgg cctgctctac gtcatcatgg tggtgtttca ggcgctgacc
     2221 ggccctaccc ctcagactac ggaggcgacc tcctcggaag aggggggaga cctgggcttg
     2281 ttgagtgagc aagcgtaagg agaagcaaca tgtcaacact ctggtttatc ctggtcgctt
     2341 ttatgctgac catgtatgtg ctgctggatg gcttcgacct gggagccggc atcattcacc
     2401 tggtcgcggc ccgcaccgat actgagcggc gtttcatctt gcgcgcaatt gggccagtat
     2461 gggacggcaa cgaggtctgg ctgatcgccg cgggcgggac gctgttcttc tctttcccac
     2521 tgctctatgc ttccagtttc agcggatttt acctgcctct tatcattgtg ctctggctcc
     2581 tgatagtgcg aggcgtcgcc atcgacctgc gctcgcgctt cgccaacagc gtctggacct
     2641 cgttctggga tggcatgttc tttctcggca gcgcgctgct ggccatcttc tacggcgcgg
     2701 ctctgggaaa cgtcattcgc ggcgtgtcgc tgaacgcgca ggggtacttc ttcgaggcgt
     2761 tttggacaga tttcaatccc ttcagcgcta atcccggtat cctggactgg tataccatcc
     2821 tgaccggcct gttagctttt acggcgctgc tcacccacgg cgcaaactac atcgctacga
     2881 agacggaggg ggcgctgaac gagcgcgccc ggcgcatctc gcgcgtcggc tggctactaa
     2941 ccgttcccct ggcggtcctc gccagtatca ccacgttctt cgtgcagccg caggtttata
     3001 cgagcttcac ccaacgccca tgggggatcg tgtttccgct tattgcgttc gtaggcttgc
     3061 ttggcgccgg cttcttcaat cttcggaagc gcgacctgcc tgccctgctc tcaactggca
     3121 cgttcatcct gggtatgctg tccagttcgg ccttcagcct gtatcctctg gtcctgccag
     3181 ccgtcaacca cgccaatagc ctgaccatcg acaacgcctc agcttcgcaa tacggcctgg
     3241 cgtttggctt gatctggtgg agtgtgggca tcattctcgc tgcgatctac ttcattatca
     3301 cctaccgccg cttttggggc aagatcacac cagaatcgag ccaggagtat taattatgat
     3361 atactagggg cagaagcaaa caacgacgtt catgcaggga gtgcaaactg gctttatgac
     3421 ggcaacatat gcaccgggcg cgcatatcga tcattatgag attattcgtt tgcttggaca
     3481 tggcggcatg aacaatgtct atcttgcgcg tgataccaat tgccaggaaa cggtcgtcct
     3541 caaattcccc aacgaagatt tgattagcaa cgtcagcgtc tttgagcgtt ataaacgcga
     3601 ggcggaaatt ggcagtcggc tcgaccatcc ccacatccaa agactgctca ataccgatga
     3661 acaacacagc gagcattacc tggtgatgga atttatccag ggcaaaaccc tgcgcgaact
     3721 gcttgatgcg ctcgcgccgc agttgctccc gccccatgaa gtggcgcgca tcggcattca
     3781 gattggtgaa gcgttgacct acgcgcacga gcatggcgtc tttcaccgcg acatcaagcc
     3841 ggaaaacgtc atgattctgg aagacggcaa tgtcaaattg atcgatttcg gtgtggcgct
     3901 gctggaaggc gcgcgccgag tgacctggcg cggtctctca ggcacggtcg gaacaccaga
     3961 ttatatgtca ccggagcagt tgacgggcga tcgaggcgcg gccggctccg atgtgtacgc
     4021 tgtcggcgcg atgctttacg agatgctctg cggccatgtg ccgttcgagg gcgaaaatgt
     4081 gtttgccgtg ctgaaccagc acgtgacgca ggacccaccc tcgatcctgc atgtcaatcc
     4141 gcatgtctcg cctgccctgg caaccgtcat tatgcgctcc atccgccgcg atcctgagaa
     4201 gcgctataaa cacatggcgg acatgctgcg cgacctccgc catttagacg cggttgttcc
     4261 ggttccctac catcccgata aacaacagta cgaaagtcgc tctcgccgca tactcctgct
     4321 gtgccttgtt acgatcgcga tttgcctgac gatcatcgcc atcggttttc tggcgcagtt
     4381 cgtgcataac gccgtgcatt agcacagacc ttgcggaaac gcgctgaaat atgctatagt
     4441 ttctatcaca gaacgctgtt tgttctcaat gagttatgct gctctcctgg aaccaatgct
     4501 gccccccttc gcatcgtttg cgcaggctgt ctacgcgact cgctttttat cacatcttca
     4561 agagccgtga gactaccgag tcctgaaatt atcctacgag gaagcgaaaa cgacttcgcg
     4621 cggcaacgtt atgccgtcaa cgctgttctg gccccaacga tggtgtcacc cggtattgtg
     4681 cagctcttat caaggagggc aagaccttgc gacacgttcg tctcaattca tccaacccgg
     4741 ctgaccctgc gcagccggtc atcgacccga cctatccact ttatgatgcc cgtttcgagc
     4801 acgacgcctg cggcaccggc ttcctcgcgc aaatttctgg cgaggccagc catgcgttgg
     4861 tacaaactgc tctggatgcg ctggcgcgcc tgacccatcg cggcgcgcaa gacgccgacg
     4921 ctgaaacaag cgatggcgcg ggtttgatga cgcaaattcc cagggattta ctgcgagagg
     4981 agttacgggc acgaggtatc tcattttcag acccggctga cctggcggtc ggaatgctct
     5041 ttttgccctc gcctgaacgt tcgcccggtg cattcgcgca cagccgtcaa attattgagg
     5101 aagtgttgca ggagcttgat agcgaatgga cggaaggcga aaagttgttc tggcgcgatg
     5161 tgcctttgaa tgtcgccata ccgggttcgc gcgctcgcgc caccatgccg catattgcgc
     5221 agatcgtgct gctccgcccg tcgcgtctcg ccctcgcgca ctatgagcaa acgctgtatt
     5281 ttgcgcgccg cttgatcgag cgcggcctgc aggcagcggg catcgccgat tgctacatcg
     5341 tctcgctgtc gcataggacg gttatctata aaggtttgct cgcgccaaac cagttgaatc
     5401 gcttctacct tgacctggcc gatgcgcgtt ataccagcgc cttcgccgtc ttccaccagc
     5461 gctacagcac caataccttc ccgtcctggc cactggccca gccgatgcgc gcgctggcgc
     5521 ataacggcga aatcaatacc ttgcagggca accgcaactg gatgcgagcc tgcgaacagg
     5581 acctcgactc gccggtgtgg ggtgacaggc tagagcagct cttgccggtc gtgcagacca
     5641 atggcagcga ctcggcgcag ctcgataaca tcctggaatg gctcgcccta tctggcaggg
     5701 atctcttgca tagtatgcag atgttgatcc cgcctgcctg ggagcatgat cctgagatgg
     5761 atagcgccca acgtgcatgg tgtgaatacc atgcagcctt aatcgatccc tgggatggcc
     5821 cggcggcgct ggctttcagc gatgggcgtg tggtaggcgc ggcgcttgac cgcaacgggc
     5881 tgcgtccggc gcggtacatg ttgacctcgc acggactgct ggtcctggca tcggaggcgg
     5941 gtgttatgcc aaccgacgcg catgatgtcg tcgaacgcgg cagacttggc cccggcgaaa
     6001 tgatcgccgt tgacctggaa cgcggcgtgt tgctgcgcga tgccgagatc aaggcggagc
     6061 tggcgcagcg ccaaccgtac cagcagtggc tggaagacaa tatgattcat ctcgatgcgc
     6121 ttccccagtt ctctcagcac tcttctttcg atcaagccga acagggtatg gatgctggcg
     6181 cgctcttcag ccgccagc
//
LOCUS       DASZAK010000833         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_589850, whole genome shotgun sequence.
ACCESSION   DASZAK010000833 DASZAK010000000
VERSION     DASZAK010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589850"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..358)
                     /gene="rnpB"
                     /locus_tag="VGT44_14675"
     ncRNA           complement(27..358)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VGT44_14675"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            599..>1528
                     /gene="selD"
                     /locus_tag="VGT44_14680"
     CDS             599..>1528
                     /gene="selD"
                     /locus_tag="VGT44_14680"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEV2582098.1"
                     /translation="MGPEALAQVLRPLTLQAVPAGLLVGLHAADDAAVYRVNDQQAII
                     STADFFPPIVDDPYVFGAIAAANALSDVYAMGGQVLMAINLVAWPDNLESALLSEILR
                     GGADTVAQAGAVIAGGHTVTDKEPKYGLAVTGMVHPEHILTKGGAQPGDLLILSKPLG
                     TGLVTTAHKFDQVQASDLEVAIQSMMQLNRNASQALAHASVGVHAVTDITGFGLLGHA
                     WEMAAQSLTGMRIEYDALPLLPGARRYAERGHITGGARRNETYLAPHIRIDERLDAID
                     REILWDPQTSGGLFAAIDPTVWPTLAALAPEVVF"
BASE COUNT          322 a          459 c          416 g          331 t
ORIGIN      
        1 gtgcctgccc ggtcttgcgg ccgcgcaggt agcaatcatc tctctaggac agcagttacc
       61 cgctgcctca agcaaccaac ccggaggttt acagacgagc agcctgcaag gacgctgcct
      121 gggcagacgt actatccctc ctatttggtc ttgctccaga tggggtttac ccagccgacc
      181 ggtcacccgg ccgctggtaa gctcttacct caccatttca cccttacccg gctgcattgg
      241 aggtgccggg cggtatgttt ctgtggcact ttccttgagg tcaccctcac tggccgttag
      301 ccagcatcct gctctgcgga gttcggactt tcctcgggcg ttgccgcccg cgattgcctg
      361 tcttactcac acccccatgt tagattaaca taagagggca aacacgtcta gcctgacaaa
      421 tctgaaggca gtcagagttt agcacattga cggccaagat acaaacaagt tacaataacg
      481 ttacttttac attcctggag tgttttatct cgctaaccgt ttggatagaa atgatatgac
      541 aacaggccga cgaattcgcc tgacatcgct cgcaagttgc gctggctgag cgtcaaaaat
      601 gggccccgaa gccctggcgc aggtcctgcg tccactcaca ttacaagctg ttcccgccgg
      661 attgctcgtc gggctgcatg ctgccgatga tgctgcagtc tatcgcgtga acgaccagca
      721 ggcaatcatt agcacagcgg atttctttcc gcctatcgtc gacgacccat atgtcttcgg
      781 agcaatcgcc gctgccaacg ccctgagcga cgtatacgct atgggcggcc aggtcttgat
      841 ggccatcaac ctcgttgcat ggcccgataa cctggagtcg gctctcctca gcgaaatcct
      901 gcgtggaggt gcagacaccg tcgcgcaggc cggagcagtc attgccggtg gccataccgt
      961 caccgataag gaaccaaaat acggacttgc cgtcacaggg atggtacatc cggagcatat
     1021 tctgacgaag ggcggcgcac agccgggcga tctactcatt ctcagcaagc cacttggcac
     1081 ggggctggtt acgacggcgc ataaattcga ccaggtgcag gcaagtgatc tggaagttgc
     1141 cattcagtct atgatgcagt taaatcgcaa cgccagccag gcacttgcac atgccagcgt
     1201 aggcgtacac gccgtgacgg acattactgg ctttggccta cttgggcacg cctgggagat
     1261 ggctgcgcag agtcttacag gcatgcgtat cgagtatgat gcgctcccac tgctccccgg
     1321 cgcacgccgt tatgcggaac gaggccatat cactggagga gcgcggcgca acgaaacgta
     1381 cctggcgccg catattcgca tcgatgagcg cctggacgcc attgaccgcg aaatactgtg
     1441 ggacccacag acctctggtg ggcttttcgc ggccatcgac cctaccgtat ggccaacgtt
     1501 agccgcgctg gctcctgaag ttgttttc
//
LOCUS       DASZAK010000834         5878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_21215, whole genome shotgun sequence.
ACCESSION   DASZAK010000834 DASZAK010000000
VERSION     DASZAK010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5878
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21215"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VGT44_14685"
     CDS             complement(<1..294)
                     /locus_tag="VGT44_14685"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV2582099.1"
                     /translation="MASRSSAAPSALAGTLGESIGEFFGTMVLILFGDGVVATVFLFA
                     NIGANNASTPFANEWVVIILGWGLAVMLGIYVAGAISGAHINPAVTLGLAATGR"
     gene            complement(447..1940)
                     /gene="glpK"
                     /locus_tag="VGT44_14690"
     CDS             complement(447..1940)
                     /gene="glpK"
                     /locus_tag="VGT44_14690"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV2582100.1"
                     /translation="MAKYVLALDQGTTSSRAIVFNHDGAIVSVAQQEFPQIYPSPGLV
                     EHDPEAIWSSQLAVARQAMQQAGASAADIAAIGITNQRETAIVWEKSTGKPVFNAIVW
                     QSRLTVPICDALKAKGFDKEIRARTGLVTDAYFSGTKVKWILDNVAGARAKADRGELL
                     FGNVDTFLMWRLSKGRVHVTDPSNASRTLMYNIYKGEWDDVILNELGVPRSMLPTVMP
                     SSAVYGETDAEFFGGAIRMAGDAGDQQAATFGQACYEPGMAKNTYGTGCFMLMNTGLK
                     GVPSQNGLLTTIGWGINNQTVYALEGSIFITGAAVQWLRDSLGIIKQSGDVEALAASV
                     PDNGGVYMVPAFVGLGAPYWDSYARGAILGLTRGSTAAHIARATLESMCYQTRDVLEA
                     MTADSGVHVKTLRVDGGAVVNNVLMQFQADILGVPVQRPVVAETTALGAAYLAGLATG
                     FWGSEAEVAEHWAIDRTFEPSMSADQRDKLYAGWKRAVERSMHWEQA"
     gene            complement(2206..2787)
                     /locus_tag="VGT44_14695"
     CDS             complement(2206..2787)
                     /locus_tag="VGT44_14695"
                     /inference="COORDINATES: protein motif:HMM:NF016216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HEV2582101.1"
                     /translation="MLTRFRGPLPKLLGQYKIIPMVEDRSHFAMALTSTQVNVIFLRH
                     CNLFELTSLMAQAERQGKAIYVNVDHIDGIHPDAAGLRYLAETFHVAGVVSNHPKTLA
                     LAKEFNLETIQRIFAADSAGLEMAIESIDTRTADLLDFSPALAIPYVIPFLDAPLPLP
                     FMAFGLAHTTQQVREIVRCGAIGVAVNRPELWQ"
     gene            3146..3724
                     /locus_tag="VGT44_14700"
     CDS             3146..3724
                     /locus_tag="VGT44_14700"
                     /inference="COORDINATES: protein motif:HMM:NF016216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HEV2582102.1"
                     /translation="MSMNKQEFLRLAHQHPVAAALKSDDDIRVAFDADAPLLFMLKGD
                     AFQLAAIISEAHRLGKRFVVHVDLVSGVGKDRAGIQYLHSIGVDAIITSRSQLIAAGK
                     AEGLITIQRLLLLDDSALETGIRTISRAAPDFVEVLPGIIFPEVAATLQHLLVGPFIA
                     GGFIRTQADVERVQRAGAILSSSSTHGLWYNR"
     gene            complement(3728..4411)
                     /gene="plsY"
                     /locus_tag="VGT44_14705"
     CDS             complement(3728..4411)
                     /gene="plsY"
                     /locus_tag="VGT44_14705"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV2582103.1"
                     /translation="MPILLVQLVLIALIGYLWGSIPAGYWMGKLLRGKNFDIRDYGSH
                     KIGATNVLRTLGRVPAAIVFIFDITKGLLPVLLALFVSFFNISGWGPTIAGLAALIGH
                     CYPVFIGFKGGRGVATGAGALLGLSPLTFGISAVTTISTIAIWRYVSLGSIVACLTTI
                     VCGIIFVFVGWVTFAQLIYMLLGPALVIALHHDNIGRLLAGTERKIGQKVEIQGNTSP
                     VDSSNPIQA"
     gene            4642..5313
                     /locus_tag="VGT44_14710"
     CDS             4642..5313
                     /locus_tag="VGT44_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2582104.1"
                     /translation="MRILIAEDDTHLGLALKKGLEDSHYAADLVKDGEEALSVALAVP
                     YDLIILDILLPQVDGFEVCKQLRDQKRQMPILFLSALSGLDHRVTGLDIGADDYLTKP
                     FAFRELEARVRALLRREGPTKSNTLVFADIKLDTRTHEVYRGSREITLTSKEYALLDL
                     LLRHPGQVLSRGMIAEHMWNIDADHLSNVIDVYIRYLRRKLCEDGEPDIIHTIRGFGY
                     QLKEP"
     gene            5316..>5878
                     /locus_tag="VGT44_14715"
     CDS             5316..>5878
                     /locus_tag="VGT44_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582105.1"
                     /translation="MFRHRRWSIPFRSLHIRAQLTLLYMLIFVLLICLLGVVFYTNLQ
                     SSLETNVDTELQEHAQEIAGGVQFVKGKLSVQGITSGFPGLIDADSGSDAPPGETATK
                     ATPTTKNTSAGSSISLSTKVDIGPLVRILDNAQTVLFVSPAFRELSVPQTSVTQALRD
                     APWIGTVMALGGEPVRLYSLPLVYKNNV"
BASE COUNT         1312 a         1583 c         1720 g         1263 t
ORIGIN      
        1 cctgccggtt gcagccagcc ctaaagtgac ggccggattg atatgtgcgc cactgatagc
       61 tccagcgacg tagataccga gcataacggc gagaccccat ccaagaatga taacgaccca
      121 ctcgtttgcg aatggagttg aggcgttgtt tgcgccaatg ttagcaaaaa ggaaaacggt
      181 cgccactacg ccatcgccga agagtatcag taccatcgta ccgaaaaact cgccaatgga
      241 ctctccgagc gttcctgcga gcgcggaggg tgcggcagaa gatcgacttg ccatagattg
      301 gttactcctt ctcctgagta aaggatggcg gcgaacaaat gctgtttgcc aaagaacaga
      361 taaggactac aagggacgca ctacaaggac ctgtctgtcg ggccgactat cctggccgtc
      421 ccgacagaca acttcaacgc atcagatcag gcctgttccc agtgcatgga gcgttcgacg
      481 gcgcgcttcc agccggcgta gagcttgtcg cgctggtcgg cgctcatgct cggctcgaag
      541 gtgcggtcga tggcccagtg ttcggcaacc tcggcctcgc tgccccagaa gccggtcgcg
      601 agaccagcga ggtaggccgc gccgagagcg gtggtctcag ccacgaccgg gcgctgcacg
      661 gggacgccga ggatatcggc ctggaactgc atcagcacgt tattgacgac cgcgccgcca
      721 tcgacgcgca gcgttttgac atgcacaccg ctgtcggcgg tcatggcttc cagcacatcg
      781 cgggtctgat agcacatcga ctcgagcgtg gcgcgagcga tatgggccgc cgtggagccg
      841 cgcgtcaggc cgagaattgc gccgcgcgcg taggaatccc agtaaggcgc gcccaggccg
      901 acgaaggcag gcaccatgta gacgccgccg ttatcaggaa cggacgcggc cagggcctca
      961 acgtcgccgc tttgcttgat gatgcccagg ctatcgcgca gccactgcac agccgcgccg
     1021 gtgatgaaga tactgccttc cagagcataa acggtctggt tattgatgcc ccagccgatg
     1081 gtagtaagca gtccgttctg cgacggcaca cccttcaagc cggtgttcat gagcatgaaa
     1141 cagccggtgc cataggtgtt cttcgccatg cccggctcgt aacatgcctg gccaaaggtg
     1201 gctgcctgct ggtcgcccgc gtcgccggcc attctgatgg caccgccgaa aaattcggcg
     1261 tcggtctcgc cgtagacggc gctggatggc atgacggtgg gcagcatcga acgtggcaca
     1321 ccgagttcat tcagaatgac atcgtcccat tcacccttgt agatgttata catcagggtg
     1381 cgtgatgcgt tcgaggggtc ggtgacgtgg acgcgccctt ttgagagacg ccacatcagg
     1441 aaggtgtcaa cgttgccgaa gagcagctcg ccgcgatcgg ctttcgcgcg cgcgcccgca
     1501 acgttatcga gaatccattt gactttggtg ccggagaaat acgcgtctgt caccaggccg
     1561 gtgcgcgcgc ggatctcttt gtcgaagccc ttagccttca atgcatcgca aataggaaca
     1621 gtcaggcggc tctgccagac gatggcattg aagacaggtt tgccggtcga tttctcccag
     1681 acgatagccg tctcacgctg gttggtgatg ccgatggcag ctatgtcagc agccgacgcg
     1741 cctgcctgct gcatcgcttg cctcgccacc gcgagctggc ttgaccagat cgcctccggg
     1801 tcatgctcga ccaatccggg cgagggataa atctgaggaa attcctgctg cgctacgctg
     1861 acaatcgctc catcatggtt gaagacgatt gcgcgcgaac tggtagtacc ctggtccagg
     1921 gcaagcacat actttgccat tggtagccct ctttctgatg tttcagacag cgaccttgct
     1981 gtatcgcccc agaatctcat ttcatcggac gagatagccc ggttcgccag acgatgattt
     2041 atattgatcg agcacaggca ctagagccgt gccttgatca caagagcagg taagataccc
     2101 cgattaccgg aagaaatgct tgctgagatg accctccttc cttgctgcct aggagatgcg
     2161 ccttctgtgc gaatgctccc gtatcttaga tgtacagatt agttgttatt gccatagttc
     2221 gggccggttt actgcgacgc caatggcccc acagcgtacg atttctcgaa cttgctgagt
     2281 ggtatgcgcc aggccaaagg ccatgaatgg caggggtaga ggagcatcaa ggaaaggaat
     2341 gacatagggt atcgctaatg cgggcgagaa gtctagcaaa tcggccgtgc gtgtgtcgat
     2401 tgactcgata gccatctcta agcccgcaga gtcggcagcg aagatacgct ggatcgtctc
     2461 caggttgaat tccttcgcca gcgcgagagt ttttggatga tttgacacca cgcctgcgac
     2521 gtgaaacgtt tcggcaagat agcgcaggcc ggcggcatcg ggatggatgc catcgatatg
     2581 atcgacattg acgtaaatgg ctttaccctg gcgttccgcc tgagccatca acgatgtcaa
     2641 ttcaaagagg ttacaatgtc gcaagaagat aacgttgact tgagtggagg taagcgccat
     2701 tgcaaaatga ctgcgatctt cgaccatcgg aatgatcttg tactgcccta atagtttagg
     2761 gagtggccca cggaacctgg taagcattct tgcacctcgc tgttgcagga aaaaaacaaa
     2821 gccctaagca gcctcataga gagggtggct caggacttct catttcactg tccttgggca
     2881 tgtatgcagt tgttcgatcg agtcgccttt gaccgtgagt gcagcgcaga gaaaacgcgc
     2941 cgacaatgga tttgtctgta tacaaatgcg tgttcctggc ttataattca agaatactca
     3001 gtaactcact gttttgtcaa gccctgttga ccttctttcg gcaactgtga tatccgtgac
     3061 tctgtggtac tagcagagtt aaggtctccg cgctgtggca caggtctcaa cagggctatc
     3121 ggagcgccaa caagagtaca acgtaatgtc gatgaacaag caggaatttc tccgcctggc
     3181 ccaccagcac ccggtggctg ccgcgctcaa gtcggacgac gatattcgcg tcgcgttcga
     3241 tgccgatgcg cccctcctct ttatgctcaa aggtgatgcg tttcagcttg ccgctattat
     3301 cagcgaggcg caccggttgg ggaagcgctt cgtagtgcat gttgacctgg ttagcggggt
     3361 agggaaagat cgcgcgggta tccaatactt gcattcgatc ggcgtggatg cgattattac
     3421 cagtcgctcg cagctcatcg cggcgggcaa agcggagggc ttgattacga tccaacgact
     3481 gctgctgctg gatgattccg cgttggaaac cggtatacgt actatctcgc gcgccgcgcc
     3541 cgactttgtc gaggtgctgc caggcatcat cttcccggag gttgccgcca cgctgcaaca
     3601 cctgctggtc ggcccgttca ttgccggcgg cttcattcgc acccaggcgg atgtcgagcg
     3661 agtacagcgg gcgggcgcca tcctgagcag cagcagcacg catggactct ggtacaaccg
     3721 gtaggtatta cgcctggatc ggatttgatg aatcgaccgg cgaggtgttt ccttgaatct
     3781 cgaccttctg cccgatcttg cgctcggtac cggccagcag tcgcccgatg ttgtcgtggt
     3841 ggagggcaat caccagtgcc gggccgagca acatgtagat cagttgcgcg aatgtgaccc
     3901 atcccacgaa gacaaaaata atgccgcaga cgatagtggt caggcaggcc acgatagatc
     3961 cgagtgatac atagcgccag atggcaatgg tgctgatagt ggtgacagcg ctgatgccga
     4021 atgtcagcgg ggaaagcccg agcagcgcac cggcgccggt agccacgccg cgtccgccct
     4081 tgaagccgat gaaaaccggg tagcaatgcc cgatcaaggc tgccagcccc gcaatggtcg
     4141 gtccccagcc ggagatgttg aaaaacgaga caaacagcgc aaggagaaca ggcagcagtc
     4201 cctttgttat gtcgaagatg aagacaatcg ctgccggtac cctgcccagt gtgcgcagca
     4261 cgttggtcgc gccgattttg tgactgccat agtcgcgaat atcgaaattc ttgccgcgta
     4321 gcaatttacc catccagtac cccgccggaa tcgatcccca caggtatccg atgagcgcaa
     4381 tcagcacgag ttgaacgaga agtatcggca tagtgatagc ctcctttgcc cggacagatc
     4441 attgtcaaac agagtatatc acagggggaa agaaggagga cgatagtggg agttcctgat
     4501 gacggcagtg gaggttccgg agggcgaccg caagggtacg ccctttggat tttccgacaa
     4561 tgagaatatt tcatcatcct ctcataatca cccggtatag tgtaggtatc aaacagacca
     4621 gaaaggggca tggtgtatac tatgcgtatc ttgattgctg aggatgatac tcacctgggg
     4681 ctggcgttaa aaaaggggtt agaagacagc cattacgcag ctgacctggt gaaggacggt
     4741 gaagaggcgc tttcggtcgc gctggccgtc ccatatgacc tgattatcct ggatattctg
     4801 ctcccgcagg tggatggctt cgaggtctgc aagcaactgc gcgaccagaa gcgtcagatg
     4861 ccgattttgt ttttatcagc gctgagcggg ctggatcatc gcgtgacggg gctggatatc
     4921 ggagccgatg attacctgac gaagccgttt gcttttcgcg agcttgaagc gcgggtgcgg
     4981 gcgctgctgc gccgcgaggg gccgacgaag agcaatacgc tcgtgttcgc cgatattaag
     5041 ctggatacgc gcacacacga ggtgtacaga ggctcgcgcg aaattacgct gaccagcaaa
     5101 gaatacgctt tgctggatct gctgttgcga catccaggcc aggtattgag tcgagggatg
     5161 attgcggaac atatgtggaa tatcgatgcc gaccatcttt ctaatgtcat cgatgtctat
     5221 atccgttacc tgcggcgtaa gttgtgcgag gatggagaac cggatatcat ccatacgatc
     5281 cgaggttttg gctatcaact caaggaaccg taatcatgtt caggcatagg cgatggtcga
     5341 tcccgttccg gtcgctccat attcgcgcac aattgactct tctctatatg ttgatcttcg
     5401 ttctgttgat ctgcctgctc ggtgtggtct tctacacgaa tctccaaagc tcgttggaga
     5461 cgaatgtcga tacagaatta caagaacacg cacaggagat tgccggaggt gtgcaatttg
     5521 tcaagggaaa actctcggtg cagggcatca ccagcggctt tccagggttg atcgatgcgg
     5581 attcgggaag tgatgcgcct cccggcgaaa cagcgacaaa ggcgacgccg acgacaaaga
     5641 atacgagcgc gggcagttcg atctcattat cgaccaaggt agatattggt ccattggtgc
     5701 gcatcctgga caatgcacaa acggttctgt ttgtatcacc cgcgtttcgc gaactgagtg
     5761 taccccaaac gagcgtgaca caggcgttgc gcgatgctcc ctggatcggt acggtgatgg
     5821 ccctcggcgg ggagccggtg cgcctgtata gcctgccgct ggtctataaa aacaatgt
//
LOCUS       DASZAK010000835         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_482764, whole genome shotgun sequence.
ACCESSION   DASZAK010000835 DASZAK010000000
VERSION     DASZAK010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482764"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VGT44_14720"
     CDS             <1..220
                     /locus_tag="VGT44_14720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582106.1"
                     /translation="GQGCGPFSGDPPSSGGPRRATMKVAPTNGDEFFVRLMPIPGYLT
                     APWWGRLEKDYESLAIFAVTRGPGSRHH"
     gene            165..1358
                     /gene="cax"
                     /locus_tag="VGT44_14725"
     CDS             165..1358
                     /gene="cax"
                     /locus_tag="VGT44_14725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HEV2582107.1"
                     /translation="MNRWLYLLLLAAPAAVITEALHVSPLVPFVLASLGIIPLAGLIG
                     LSTEALAERLGHRIGGLLNATFGNAAEIIIGLTALAAGLPEVVRASLAGSIIGNVLLV
                     LGSAMMVGGWRYRRQRFDPRVAGQYATMLTLVVVGMAIPSLLATVGESTRPGAAVVQG
                     SELHQLSIGIALILLFCYVAYIAYSVFGLRASPKDVLPISISTARDTAVENPNPAQSS
                     TPAVETAPQQRATTAGLLATLAAWWRTSLWFPIAVLAGVTVVTAFVSEVLVSTIEPVA
                     HQIGLSPFFVGLIILPIVGNAAEHFSAITSAARNHMEISMAITAGSSIQIALLAAPIL
                     VLAGPIVGVPLDLNFSLLEVALFALVTGLYALISLDGESTWLEGLLLCAFYLILAVGT
                     FFTPA"
     gene            1497..2099
                     /locus_tag="VGT44_14730"
     CDS             1497..2099
                     /locus_tag="VGT44_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV2582108.1"
                     /translation="MSKVVVFTNLTLDGIMQAPGRPDEDRRGGFEHGGWAAPYAAMSQ
                     AGDSMANVGALLLGRRTYEDFYAVWPKRKEDPISAMLDNMQKYVMSRTLSEPLSWINS
                     TLLKGDASETVARLKGKPGKDLVIMGSGELAQSLMRSNLIDEYVLLIHPLVLGSGRRL
                     FPDGSAFALRLLDTRTTNNGVVIVTYQPAEPTARKTTEAE"
BASE COUNT          445 a          647 c          647 g          496 t
ORIGIN      
        1 ggggcagggc tgcggcccat tcagcggtga tccaccgtct tcaggcggcc cacgacgggc
       61 gaccatgaag gtcgccccta ccaatggtga cgagtttttc gttagattga tgcctattcc
      121 tggctatctt acagcgccct ggtgggggag attggagaaa gattatgaat cgctggctat
      181 atttgctgtt actcgcggcc ccggcagccg tcatcactga ggcgctgcac gtgtcacccc
      241 ttgttccgtt tgtgcttgcg tcgctaggaa tcatcccgct tgccggattg atcggcttat
      301 ctaccgaggc actggcggag cgccttgggc atcgcatagg gggtctgctg aatgcgacct
      361 tcggcaacgc ggcggagatt atcattggct tgaccgcgct ggcggcgggg ctgcccgagg
      421 tggtacgcgc ctcgctcgcc ggctccatca ttggaaatgt cctgcttgtg ctgggttcag
      481 ccatgatggt tgggggatgg cgctaccggc ggcagcggtt tgaccctcgc gtggctggtc
      541 agtacgcgac catgctcacc ctggtggtgg tcggcatggc catcccctcg ttgctcgcga
      601 cagtcggtga gagcacgcgc ccaggagccg ctgtggtaca gggtagcgag ctgcaccagc
      661 tcagcattgg gatcgcgctc atcctgctgt tctgctacgt agcgtatatc gcctactctg
      721 tcttcggctt gcgtgcatct cccaaagatg tgcttcccat atcgatatct acggcgaggg
      781 acaccgctgt tgagaatccg aaccctgccc agtcatcaac gcctgcagtt gaaaccgctc
      841 cacagcagcg agcaacgact gccgggctgt tggcgacact tgccgcctgg tggaggacct
      901 ccctctggtt ccctatcgcc gtcctggccg gggtcacggt ggtcaccgct tttgtgagcg
      961 aggtactggt gagcactatc gagccggtag cacatcagat cgggctgagc ccgttctttg
     1021 tcggtctcat tatcctgccg atcgtgggca acgcggctga gcacttttct gccatcactt
     1081 cggctgcccg caatcacatg gagatctcga tggctattac cgctggctcg tcaatccaga
     1141 ttgcgctgct cgccgcgcct atccttgtgc tcgctggccc tatcgttggc gtaccgctcg
     1201 atctcaattt ctccctactt gaagtggcac tatttgccct ggtgactgga ctctacgcgc
     1261 tcatcagcct ggacggggaa tccacctggc tcgaagggct gctgctctgc gccttctacc
     1321 tcattctcgc ggtgggcacc ttttttacac cagcctgata gcatgaggta taaatctaag
     1381 aagaactgcg aaaaaacgaa aagatttgga acaaatattc taaaaaagac tctggtgtga
     1441 taaaataaga gggttgcaaa tagatacaac taacgagaat gaagaggtaa ctacttatga
     1501 gcaaagtcgt tgtcttcacg aatctgacgc tcgacgggat catgcaggcg ccggggcggc
     1561 cagacgagga ccgccgcgga ggcttcgaac acggcggctg ggcggcgccc tacgccgcga
     1621 tgtcgcaggc tggggacagc atggccaatg tcggtgccct gctgttaggc cggaggacct
     1681 acgaggactt ctacgccgtc tggcccaagc ggaaggaaga ccctatctcg gcgatgctag
     1741 acaatatgca gaagtacgtc atgtcaagaa cgctctctga gccgctctcg tggatcaact
     1801 ccacgctcct gaagggcgac gcctcggaga ccgtggccag gctcaaaggg aagccaggaa
     1861 aggatctcgt gatcatgggc agcggagagt tggcccagtc gctgatgcgg agcaacctca
     1921 tcgacgagta cgtgctcctg atccacccgc tagttctggg atcggggcgt cgcctcttcc
     1981 ccgacggcag tgcattcgct ctccggctcc tcgacaccag gacgacgaac aacggcgttg
     2041 tgatcgtgac ctaccagccg gccgaaccga cggcgaggaa gaccaccgag gcggaatgac
     2101 tccattccca catagatctc cctccaaaat catattgtaa aaatgcactt cagatacgtt
     2161 tgtcatttag caaaggtatt cgacatagta ttttctctat gagtatgatc tccatgtaca
     2221 cagctacatg cagcg
//
LOCUS       DASZAK010000836         5195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_696237, whole genome shotgun sequence.
ACCESSION   DASZAK010000836 DASZAK010000000
VERSION     DASZAK010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696237"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VGT44_14735"
     CDS             <1..505
                     /locus_tag="VGT44_14735"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HEV2582109.1"
                     /translation="GEPTVYSGRVLSTAGTPIPGALLDVWETDENGLYEQQDPQQPDM
                     NLRGKFRADGEGRYSFVGIKPVSYPIPDDGPAGQLLRALGRHPFRPAHIHMIVSADGF
                     APVTTHLFVKGDPYLDSDAVFGTKDSLVVAFIRHNSLDEAARYHVAAPFYMVEYDFVL
                     KPSSRNG"
     gene            528..1919
                     /locus_tag="VGT44_14740"
     CDS             528..1919
                     /locus_tag="VGT44_14740"
                     /inference="COORDINATES: protein motif:HMM:NF024826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SidA/IucD/PvdA family monooxygenase"
                     /protein_id="HEV2582110.1"
                     /translation="MTEDRKQPSAGPFAEQAAESIPTLLVIGAGPKAIAIGAKRHMLA
                     KLGYPVPHLHIIDRQGVATNWSGKAGYTDGSQFLGTRPEKDVGFPYLSACWGDKALSH
                     AVAKEMQHLSWQSYLIDQMRYSSWIDRGRTRPTHGEWSQYIQWVAEKVELAPCIAEVT
                     TIGLTADQQRWQLTCQPTDASAPLTLTGDGLVITGPGTPLTVPGQPKEHPRIIDGATF
                     WLRIEEFEQLRSQITKPLNIGVIGTGETAAAIVVALVDTLRSSAFIEVISPYGVIYSR
                     DEGFEENRLFSDPDGKLAKLYGDHKHAANWLQLSERDRREFVRRTDRGVFSLKAIEEI
                     SNAENVRSVLGTVRRIHASDTMVRVESEYDGTLNHDEYDYVVVARGFDALWFRHLLND
                     ATRDRMQVVTQGFDSKAIERSITVDLSLDGFKPKLHLPMLAGIAQGPGFPNLSCLGLL
                     ADRILASYSPSGE"
     gene            complement(1929..2108)
                     /locus_tag="VGT44_14745"
     CDS             complement(1929..2108)
                     /locus_tag="VGT44_14745"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEV2582111.1"
                     /translation="MQSVKTTRNPPNWLDRETRALVKDIIRLLKARHPDLLAIILYGS
                     VARHEERPLPAPLTA"
     gene            2504..4441
                     /locus_tag="VGT44_14750"
     CDS             2504..4441
                     /locus_tag="VGT44_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582112.1"
                     /translation="MPRKHKQQENHTPDPFEDMRPSEKLNGEESDGPQEEFAQEYPSR
                     HLSSPFYEGEPTHEAGKRDVELYIRTYNTLLRSSGEVSLKALVQAHYNIDSSLHPDAR
                     SPYPDMSAFIYSVLRLPSAIVSCELVLLGQSEDVFLQQGYQVDSWEGVTASARRRKWF
                     YDGKQTLAVYVASVSDTDDIVPILVAFQIEWNKMYYLLNADPTTMQLLETRMDRTSPV
                     FTEISKVLRERLHIPVDDWERLELIWGDRLWDNMLRIGRHRKSFGLRFLGGSHVGYVR
                     ATRQWWAPVEQLFNTLGLQDRPVYFVSSNTHSLLNLVSGSILRRDTELTKFALSGEDP
                     YLADECRKLQQGIVPGNWQNFLYFTTRSWMQTPAGREFAKTRIQEEQERGIWHVTARH
                     GLEIDAQIFDLAKLRHMEIDPRCRMPGMDKLAKSEAIILNIDYPLGMAAYRMMREIME
                     NLTNIKGVYILGKAATLNGSIGDVMISNVVLDEHSQNTYWLDNCFIAQDVQPYLIYGS
                     VLDNQKAVSGKGTYLQNRQYYDFYYHANYTVIEMESGPYLNAIYEDQYLTRYPVGENI
                     NFARLPYDLGILHYGSDTPYTRGKNLGAGSLSYFGMDSTYACTIAILRRLFENEIRSI
                     YQSESGPLELAMPEQTKVSPS"
     gene            complement(4445..4891)
                     /locus_tag="VGT44_14755"
     CDS             complement(4445..4891)
                     /locus_tag="VGT44_14755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582113.1"
                     /translation="MKDIRRDEGDWSGRAGGDLAVSEEQERAPEIGKRSIIRCSICRK
                     RAWLDTTYFLEEPPGVPDPHQSWTLCKRCHAALLVEMERSPVLSPLRLRIAVGIVAAE
                     RSPDIYAPTRAPISDRTWIVVMAWGFAIAMVLHLAIIVMLAFVAGH"
BASE COUNT         1204 a         1516 c         1394 g         1081 t
ORIGIN      
        1 cggcgaacca accgtgtact ccgggcgcgt gctgtccacc gccgggacgc ctatccccgg
       61 cgcgctgtta gatgtctggg agaccgacga gaatggcctg tacgagcagc aggacccaca
      121 gcagcccgac atgaacctgc gcggcaaatt ccgcgccgat ggcgaggggc gctacagctt
      181 cgtgggcatc aaacccgtct cctaccccat ccccgacgat gggccagccg ggcaactgct
      241 gcgcgcgctg gggcggcatc ccttccggcc cgcccacatc cacatgatcg tctccgccga
      301 cggctttgcg ccggtcacaa cccacctgtt cgtcaagggc gatccctacc tcgactcaga
      361 tgccgtcttc ggcacaaaag attcgctcgt cgtcgcattc atccggcaca attccctgga
      421 tgaagcggcc cgttatcacg tcgccgcgcc attttacatg gtggagtacg atttcgtctt
      481 gaagcccagt agcaggaacg gttagatcga gaaagagaaa tttattcgtg accgaggaca
      541 ggaagcaacc ttcagcaggc ccgttcgcag agcaggctgc cgaatctatt ccgacattgt
      601 tggtaatagg tgccgggcca aaggccattg cgatcggggc gaaaaggcat atgctggcga
      661 aattgggata tcctgtgccg catctgcata tcatcgatcg gcaaggcgtc gcgactaatt
      721 ggtcaggtaa ggcaggatac accgatggga gtcaatttct gggaacgcgc ccggagaagg
      781 atgtcggttt cccctatctc tccgcctgct ggggagacaa agcgctcagt cacgccgttg
      841 caaaggagat gcagcacctc tcatggcaaa gttatcttat cgaccagatg cgctactcaa
      901 gctggatcga ccggggacgc acacgcccaa cgcacggcga atggtctcaa tacatccaat
      961 gggtagccga gaaggtggag cttgctccct gtatagcaga ggtaacaacc attgggctca
     1021 ccgccgatca gcagcgctgg caactgacct gccaacccac cgatgcaagc gcgcctttga
     1081 cgctgaccgg tgatggcctg gtaataaccg gtcccggcac acccctcacc gtcccaggcc
     1141 agccaaagga gcatccacgc attatcgatg gcgcaacatt ctggctgcgc atcgaggaat
     1201 tcgagcaact tcgttcccag ataacgaaac cgctcaatat cggcgtaatc ggcaccggcg
     1261 aaactgccgc agcaatcgtt gtagcgttgg tagacacgct cagaagttcg gcatttatcg
     1321 aggtcatcag cccctatggc gtcatctact cgcgcgacga gggctttgaa gagaaccggc
     1381 tcttctcaga tcccgatggc aagctggcaa aactctacgg cgaccataaa cacgcggcga
     1441 actggctgca actgagcgag cgagatcggc gcgaattcgt caggcgcacc gatagaggcg
     1501 tcttctcact gaaagcaatc gaggaaatca gtaacgccga gaatgtacgt tctgtcctcg
     1561 gcaccgtgcg gcgaatccac gcctctgata cgatggtgcg cgtcgagagc gaatacgatg
     1621 gcacactgaa ccacgacgag tacgattacg tcgttgtcgc tcgcggcttc gatgccctct
     1681 ggtttcggca tctcctcaat gatgcaactc gcgaccgcat gcaagtagtc actcaggggt
     1741 ttgatagcaa agccatcgag cgctccatca cggtagacct ttcccttgat ggattcaagc
     1801 cgaaattgca tctgcctatg ctggcaggca ttgctcaagg gccgggtttc cccaatctca
     1861 gttgcctggg tctactggca gaccgtatcc tggcctccta cagtccatcc ggggaataac
     1921 ttcgatattc acgccgtgag cggcgcggga agcggtcgct cttcatgccg agcgaccgac
     1981 ccatacagaa tgattgccag caagtcggga tgcctcgctt tcaaaagcct gatgatatct
     2041 ttgacaagag cgcgtgtttc tctatccagc cagttggggg gatttcttgt agttttcact
     2101 gattgcatag cagcatcgcc ttcccgctaa ttatgacgag gcccacgaat gaattttctc
     2161 tggcggattt cggcaatcat accagattct ttcgccggtt gcaaaaccga ctaacaaccc
     2221 agggcgaccg caagggtctc cactgcacca tcggcgatat ccgtatagag gtagaggcat
     2281 tactaggggg aaacgccagg tatggggatg cccgacgtta tagtaatacc tctttatgta
     2341 ggaacgtttt acttgcatga ttggtggcat aaagtatgca tgaaatgaac aatcgcctga
     2401 atgggtgaaa aaggccctac catatcgttc agtcagcgag tataataatc gaaaataatc
     2461 aaagcgtcat catctcaatt cactcgaaga agaggaacaa cacatgccac gcaaacataa
     2521 acaacaggag aatcatactc ccgacccttt tgaggatatg cgcccatccg agaaactcaa
     2581 tggcgaggaa agcgacggtc cgcaggaaga gttcgcgcaa gagtatccat cgcgccacct
     2641 gagcagcccc ttttacgaag gcgaacccac acacgaagcg ggcaagcgcg atgtcgagtt
     2701 atatatccgc acctacaaca cattgttgcg cagttccggc gaagtcagtc tgaaagcgct
     2761 cgtacaggcg cattataata tcgactcgag cttgcatccc gatgctcgct cgccctaccc
     2821 ggacatgtcc gctttcatct acagcgtact gcgcctgcct tccgcgattg tctcgtgcga
     2881 gctggtgctg ctgggccaat ccgaggatgt attcctgcaa cagggatacc aggtggatag
     2941 ctgggaagga gtgacggcct cggcgcggag gcgcaaatgg ttctatgatg gcaagcagac
     3001 gctggcagtc tacgtcgcca gcgtgagcga caccgatgat atcgtgccta ttctggtcgc
     3061 cttccagatc gaatggaaca agatgtatta cctgctcaat gccgacccaa ctaccatgca
     3121 actgctcgaa acgcgcatgg accgcacgtc gccggtcttc accgagatca gcaaagtgct
     3181 gcgcgaacgg ctgcatatcc cggtcgatga ctgggagcgc ctggaactga tctggggcga
     3241 tcgcctgtgg gacaatatgc tgaggatagg taggcatcgc aagagctttg gcctgcgctt
     3301 cctgggcggt tcgcacgtcg gctacgttcg cgccacacgg cagtggtggg cgccggttga
     3361 gcaactattc aatacgctcg gcttacaaga ccggcctgtc tacttcgtct ccagcaatac
     3421 tcatagcttg ctgaacctgg tatcagggtc gatcctgcgc cgtgacaccg agttgacaaa
     3481 gttcgcgctg agcggcgaag atccctacct tgccgacgaa tgccgtaaat tgcagcaggg
     3541 cattgtcccc ggtaactggc aaaacttcct gtacttcacc acgcgctcct ggatgcaaac
     3601 tcccgcagga agggaattcg cgaaaacgcg tatccaggag gagcaggagc gcggcatctg
     3661 gcacgtcacc gcccggcacg gcctggagat cgacgcgcag atctttgacc tggcaaaatt
     3721 gcgccacatg gaaatcgacc cgcgctgccg tatgcccggc atggacaagc tggctaaaag
     3781 tgaggccatc atcctcaaca tcgattaccc gctcggcatg gcggcctatc gtatgatgcg
     3841 cgaaatcatg gagaacctga ccaacatcaa gggtgtctac attctgggca aggccgcaac
     3901 tctgaacggc agcatcggcg atgttatgat ctccaacgtg gtccttgacg aacacagcca
     3961 gaatacctac tggctcgata actgctttat cgcccaggac gtgcagccct acctgatcta
     4021 tggctcggtg ctggacaacc agaaagctgt ttcgggcaaa ggcacctacc tgcaaaatcg
     4081 ccagtactac gatttttact accacgccaa ctataccgtc atcgaaatgg agagcggccc
     4141 gtacctgaac gctatctacg aagatcagta cctgacgcgc tatcccgtcg gcgaaaacat
     4201 caacttcgcg cgcctgccct acgatctagg catcctgcac tacggctcgg atacgcccta
     4261 tacacgcggc aaaaatctgg gtgcaggcag cctctcgtac ttcggcatgg actcgaccta
     4321 tgcctgtacc atcgccatcc tgcgtcgcct gttcgagaat gagattcgct ccatctatca
     4381 atccgaatcg ggcccgttgg agttggccat gccggagcaa accaaagtct cgccgtcgta
     4441 ggggtcaatg gcctgctacg aaggccagca tcacgatgat cgccaagtgc agcaccatcg
     4501 caatcgcgaa accccaggcc atcaccacga tccaggtacg gtcggatatc ggcgcgcgcg
     4561 tgggcgcgta gatatcgggt gagcgctccg ctgcaacgat gccgacagcg atgcgcaggc
     4621 gcaggggaga tagcaccggc gagcgttcca tttcgaccag caaggccgcg tggcaacgct
     4681 tgcagagcgt ccaactctga tgtgggtcag gcacgccggg tggttcttcg aggaaatagg
     4741 tggtatctaa ccacgcgcgt ttccgacaga tagaacagcg aatgattgag cgctttccga
     4801 tctctggcgc gcgctcttgc tcctcactga cggccagatc gccgcctgcc cgtccagacc
     4861 agtctccttc gtctcttcgg atatctttca cgtttcgctg ccctttctgg ggatcgtggc
     4921 actacctgga cgacggtcgt aacgtctcac atatcctact tcataggttg gattatacca
     4981 tatcggacag gtgatttcct acgcgcggca ggcgttatat gtcaatttct gccatttttc
     5041 gctttccagc tatcaaccta ttttcttttg aaaagagata tgctacaatg accacgtggt
     5101 tcagcatagg tagattctat cgaggccctg gtttcgctcc gtcacctggt atctaggtaa
     5161 actgcctgag aggaagtaat gtaacttccc caacc
//
LOCUS       DASZAK010000837         3648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_482899, whole genome shotgun sequence.
ACCESSION   DASZAK010000837 DASZAK010000000
VERSION     DASZAK010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482899"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="VGT44_14760"
     CDS             <1..527
                     /locus_tag="VGT44_14760"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582114.1"
                     /translation="QRLGFDPGDVEYVPESHRAYTATCAWIKLKGGSLGIMGEVDPQV
                     LLNFDLPGELRVYAAQMHIRPLVRPGWQVQPIAPISNYPPVVEDLAFVVAEEITNTQL
                     LDAIRKAGGTLLARAELFDIYRGKPIPDGHKSMAYKLTYESVEAPLKEAQVVALRNRI
                     IRRVGEAVGGTLRV"
     gene            700..2238
                     /locus_tag="VGT44_14765"
     CDS             700..2238
                     /locus_tag="VGT44_14765"
                     /inference="COORDINATES: protein motif:HMM:NF013568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582115.1"
                     /translation="MNENDVVLSDNEITMLLALAAVVGEEARPVERLIEGGQLSYAQE
                     LSAIGYLCAKGLAESVEIGAETRRVLRKAGQEYDEQGLPERRLWQHLQAKGALTLQEV
                     SAFLPQSEAKFTIGMYLGAFQKAGYVAFANGAITPRQQEMPGEIADLQRYLGSVKETG
                     ERDSHNPAEGEAEKRKLVEDKRTPLPGYRLTEAGRAQALLYAGAEQGALIGAVTREML
                     LSGAWRSRQFRPLSVVAPPEKRLEMQHPLPRFLAYLRSALTGAGYREVSRPILETQFW
                     NLNVLYMQKCHPVRSPKHLLAIDGVTLPEESPAFRAVERACQERFASEYQGLGSSGSR
                     GWGDLDGFKHDQVVVRSHSTPVTVRQLATARTYPLRVFGFSRCCRARPEKPEFLQMDV
                     LVADEGLNVSTLLGTIKHVCLAMFPRAVAIRVQANYFPFAEVSVDIWVVYADGSSDEI
                     GSCGLLRPESLAILDVPVPVGLIGFNVSRLARCLCENARALDPNSIGFDAFDETEIPV
                     YAER"
     gene            2225..>3648
                     /locus_tag="VGT44_14770"
     CDS             2225..>3648
                     /locus_tag="VGT44_14770"
                     /inference="COORDINATES: protein motif:HMM:NF036799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582116.1"
                     /translation="MPNVDFERARLARRLAPTLDLPDFAYRLLSQKALVDNQNDETLT
                     INFMDTLRPELWCVEGLARHLAPRAGSQYGLALIEQLPRVAARFEEKEKRSAWVLYVN
                     VEAGQQIIDDGYFLKKVRDQLGFGSRRQIDVTILPGMQREIALYAGSDEDDDLIPELR
                     LSPGMNSVVVSALDPRIAAWFCFTLACNYIDRGNTLTGYAGPPGAQAMMAKWDAFTFD
                     DAYVRKMLGFMPDASHFQEMLRGQHFDVHHDGQVWRVTPPIFRFDIIHPIDIIEEYLV
                     VTHYDEARPTPLYAPPGMGSQTRRSALEQRLVTYMQAYGARQTIGLLLDSYDNVIGHM
                     RHDAPGQLMRLENAVNKNREYLVDSSLPPLLRNAVHPSAPQPPTTFYLFTETACLDGD
                     NVAHSRWKMGILLVGSDHPFNNAHTLLDALCHHLRLPYTLKRGSSPTLIPGRQMQVCA
                     GDASIGQFGEVHPEVLTNWDLFYPA"
BASE COUNT          699 a         1063 c         1176 g          710 t
ORIGIN      
        1 tgcagcgact cggttttgat cctggcgatg tcgaatacgt ccctgagagc caccgggcct
       61 ataccgcgac ctgcgcctgg atcaagctga agggcgggtc gctaggtatc atgggcgagg
      121 ttgacccgca ggtgctgctc aacttcgatc tgcccggtga gctgcgcgtc tacgcggcgc
      181 aaatgcacat caggccgctc gtcaggccgg gctggcaggt gcagccaatc gcgccgatca
      241 gcaactatcc acccgtcgtg gaagacctgg ccttcgtcgt ggccgaagag atcaccaaca
      301 cgcagctgct cgatgccatc cgcaaggctg gcggaacgct gctcgcacgc gccgaactct
      361 tcgacatcta ccggggcaaa cccatccccg acggccacaa atcgatggcc tacaagctga
      421 cctacgaaag tgtggaagcg ccgctcaagg aggcgcaggt agtggcgctg cgcaaccgca
      481 tcatccgacg tgtcggcgaa gcggtgggtg ggacgctgcg ggtgtgagcg ttgctgcatc
      541 tgacaccagt gtcccgattt cgtaggggtc aatcatgaat ttgaccaacc agggtcggtc
      601 agagtgtgtc accctgagcg cagcgaaggg tctcggtgaa tggaccgcga gatgcttcgc
      661 tgcgctcagc atgacattcc tcgtagatag tgagttataa tgaacgaaaa cgatgttgtc
      721 ctcagcgaca acgaaataac tatgctgctt gcgcttgcag cggttgtcgg cgaagaggcg
      781 cggcctgtcg agcggttgat cgagggcgga caactgagct atgcgcagga gctgtctgct
      841 atcgggtatc tgtgcgcgaa ggggttggcg gaaagtgtag agatcggcgc ggagacgagg
      901 cgcgtgcttc gcaaggcggg gcaggaatac gatgagcagg ggctgccgga gcggcgcttg
      961 tggcagcatt tgcaggcaaa gggcgcgctg acgttgcaag aggtcagcgc ctttctgccg
     1021 cagtcggagg cgaagtttac gattggtatg tacctcggcg cgttccagaa ggccgggtat
     1081 gtagccttcg ccaatggcgc gatcacgccg cgccagcaag agatgcccgg cgaaatcgcc
     1141 gatttgcagc gctacctcgg ttctgtcaag gagaccggcg agcgcgattc gcacaatccg
     1201 gcggagggcg aggcggagaa gcgcaagctg gttgaggata agcgcacgcc cctgccgggc
     1261 tatcgcctga ctgaggcggg acgcgcgcag gcgttgctct atgccggagc ggaacagggc
     1321 gcgctcatcg gtgcggtcac acgcgaaatg ctcctgagcg gcgcgtggcg cagtcgccag
     1381 ttccgtcctt tgagcgtggt cgcgccgcca gagaaacggc tggaaatgca gcatcccctg
     1441 ccgcgtttcc tggcttattt gcgcagcgcg ctgacggggg cgggttatcg cgaggtatcg
     1501 cggcccattc tggagacgca gttctggaac ctgaacgtgc tgtatatgca gaagtgccac
     1561 ccggtgcgca gccccaaaca cctgctggcc atcgacggcg tgacgctgcc ggaagagagt
     1621 cccgccttcc gcgcggtcga gcgcgcctgc caggaacgct ttgccagcga ataccaggga
     1681 ctcggaagtt ccggatcgcg cggctggggc gacctggatg gcttcaagca tgaccaggtg
     1741 gttgtgcgca gccacagcac gcccgttacc gtgcgccagc ttgcgacggc tcggacgtat
     1801 ccattgcgcg tctttggctt ctcgcgctgc tgtcgcgccc gcccggaaaa gccggaattc
     1861 ttgcagatgg atgtgctggt agccgacgag ggattgaatg tttcaacgct gctgggaacg
     1921 atcaaacatg tctgcctggc gatgtttccc agggcggtcg ctattcgcgt ccaggccaac
     1981 tatttcccct ttgccgaggt ctcggtcgat atctgggtcg tgtatgccga tggcagcagc
     2041 gacgagatcg gctcgtgtgg tttgctgcgc ccggagtcgc tggcaattct ggatgtgcct
     2101 gtgccggtcg ggctgatcgg cttcaacgtc agccgcctgg cgcgctgcct gtgcgagaac
     2161 gcgcgggcgc tcgatcccaa cagcatcggc ttcgatgcct ttgatgaaac ggagataccc
     2221 gtctatgccg aacgttgatt tcgagcgcgc gcggcttgcc cggcgccttg ctcccacgct
     2281 agacctgccc gatttcgcct atcgcctgct gtcgcagaaa gccctggttg acaaccagaa
     2341 cgacgagaca ctgacaatca acttcatgga taccttgcgc cccgaactgt ggtgcgtgga
     2401 agggctggcg cgccatcttg ccccgcgagc aggtagccag tatgggcttg cgttgatcga
     2461 acagctgccc agggtagcgg cacgtttcga ggagaaagaa aagcgatctg cgtgggtgtt
     2521 gtacgtcaat gttgaggcgg gtcaacagat catagatgat ggctatttcc tgaaaaaggt
     2581 gcgcgaccag ctcggtttcg gcagccgccg gcagatcgac gtgaccattc tgccgggaat
     2641 gcaacgcgag attgcgcttt atgccggcag cgacgaggat gacgacttga ttccagaatt
     2701 gcggctctcg ccgggcatga atagcgtcgt cgtctccgcg cttgacccgc gtatcgccgc
     2761 gtggttctgc ttcacattag cctgcaacta catcgatcgc ggcaacacgc tgaccggcta
     2821 tgcaggcccg cccggtgcgc aggcgatgat ggcgaaatgg gatgccttta cgttcgatga
     2881 tgcctatgtg cggaagatgc tcggttttat gcccgacgcc agccattttc aggagatgct
     2941 gcggggccag catttcgacg ttcaccatga tgggcaagta tggcgggtga cgccgcccat
     3001 cttccggttc gatatcattc atcctatcga tatcatcgaa gaatacctgg tcgtcacaca
     3061 ctatgacgag gcgaggccga cgccgctcta tgcgccgccg ggcatggggt cacagaccag
     3121 gcgcagcgcg ctggagcagc ggctggtgac atacatgcaa gcctatggcg cgcggcagac
     3181 gattggactg ctgctcgact cctacgataa tgtgatcggc cacatgcggc acgacgcgcc
     3241 gggacagctt atgcgcctgg aaaatgccgt caataaaaac cgcgaatacc tggtggatag
     3301 cagcctgccg ccgctgctgc gcaacgccgt gcatcccagc gcgccgcagc cgcccacgac
     3361 cttttacctc ttcaccgaaa cggcttgcct ggatggtgac aacgtagcgc attcgcgctg
     3421 gaagatgggc attctgctgg tgggaagcga tcaccctttc aataatgcgc acacgctgct
     3481 cgatgcgctc tgccatcacc tgcgcctgcc ctacacgctc aaacgcggca gcagcccgac
     3541 gctgattccc ggtcggcaga tgcaggtatg tgcgggggac gcctcgatag ggcagtttgg
     3601 cgaggtccat ccagaggtgt tgacgaactg ggacttgttc tatcccgc
//
LOCUS       DASZAK010000838         5977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_449027, whole genome shotgun sequence.
ACCESSION   DASZAK010000838 DASZAK010000000
VERSION     DASZAK010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5977
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449027"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..1143
                     /locus_tag="VGT44_14775"
     CDS             190..1143
                     /locus_tag="VGT44_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2582117.1"
                     /translation="MKAVQLAQYGGPEVLQVQEVPDPVPGPQDVLVKVAATTVNRLDL
                     FQRDGSRPTGKLPFTPGLEAVGTVLADNAGFRAGERVMTTWAQAAKGGGGYAGIIAAT
                     ASTLIRIPDNVSFEEAVATGLVSSTAWGALFDVGGLQRGESVLIWAGSSGVGTIAIQM
                     AKHAGCKVFTTASSVEKAAKLRELGADVVVNHKTEDVAQVVRSAGGVNLVIELVGTTL
                     QTSIDACANGGRVVLIGNLGGQNATVDTQSWRLKLVNVLGAGIRHTSVENERRILELV
                     AEKAIRPVIGLTLLVEQAAEAHHILAKNEIMGKIVLMHEKP"
     gene            1153..1743
                     /locus_tag="VGT44_14780"
     CDS             1153..1743
                     /locus_tag="VGT44_14780"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEV2582118.1"
                     /translation="MSAHKFRTFLPLFDELRGERVIVRPYRESDAQALQEAVAESRDN
                     LRPWMPFADEHQTVDESRDWIIHQIAGWLTRESLVVGFWDAATQRYLGGSGYHVRDWE
                     IGYYEIGYWIRVTAEGHGYVTETIRLLTDYAFETLKANRLEIRCDELNVRSAAIPRRL
                     GYIEEGLLRNDTITPGGDLRNTLVFSLITGEWPRER"
     gene            2128..3522
                     /locus_tag="VGT44_14785"
     CDS             2128..3522
                     /locus_tag="VGT44_14785"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV2582119.1"
                     /translation="MSRIRWRVVSLVLLLSSLLLALFLSPIFHSQASASLVQLSSDPY
                     TNTTTKHSTEVSDASYSYGSTIVSAFQVGKSYNSGGASNIGWATSTDSGATWTNGFLP
                     NITKVAGGPYQRAGDTGVTYDAAHGTWMIVTNGFYNEAFAILVSLSSDGKNWSNPVAV
                     IAVPKSNTNDFDKSGIACDNTSTSPYYGHCYVNWVNEANHDLIAMSTSTDGGLTWSAP
                     LPTADSADGFNGHPFVQPDGTVVVVINKRIFAPPQTTHILAFTSTDGGASWSSTVTVV
                     AKSQNHPNSGGIRDLHYFTAGMDGAGKLYVVWNDCRYESGCTANDLLMITSTNGTTWS
                     APQRIPIESVGSGVDHFIPGLGVDASTSGSTAHLGLAYYYYPVANCTSATCQVDVGYV
                     SSTDGGNTWSSPAQLDGPMSVTWFVNTQWGYMPGDYITTSFAGGKAFPVFSVASAPNG
                     STYDQTTDTVVNGL"
     gene            3608..5029
                     /locus_tag="VGT44_14790"
     CDS             3608..5029
                     /locus_tag="VGT44_14790"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2582120.1"
                     /translation="MKYRSGLSRRNVAIGSLELAIILFALVILIAPQVPSRAAGTGDV
                     PTYMGSNTRDGYYPNETGINPGNASRLAIKWKLQAGGSIDVQPVILNGIIYWGSWDGY
                     EHATRASDGAEIWKTNIGKTISAGTCTPKNAGPAGTATIVQMAVNGATEVVFVPGGDT
                     AFYALDAASGHILWKTSLGASPNEMLWGGSAVYNGNVYTGISSYGDCPLTPGHVFELS
                     GTTGAILHQFNTTAHNCPGAGAWDAPTIDETAGTLFITTGTIASCNPSEIYAYALLEL
                     RASDLALLGHWQIPKSQQTGDGDFGSTATLFTATINGTLHQMIGTVDKNGIYYAFDRN
                     DISAGPLWERVVGMIKNHDKSSRASSAWDGSTLYEGNTTTTINGQSCKGSVRAINPAN
                     GAFLWEYCAPGKVTAGLTVVPGLVIVGAYSDLVVLNAATGKKLFLYHDPSALAEFWGT
                     VTVSNGVVYVGNRDGALYAFVVV"
     gene            5253..5897
                     /locus_tag="VGT44_14795"
     CDS             5253..5897
                     /locus_tag="VGT44_14795"
                     /inference="COORDINATES: protein motif:HMM:NF013695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV2582121.1"
                     /translation="MSVNVSRAIEVRSGIRIEVFTVIWMVIEAAVSIGAGIIAGSTLL
                     TAFGLDSVIELVSGTILLWRLLIEAREGDTERVERAEHRAAWIVALSLALLCAYVFGT
                     ALYGLLTHSMPESSPVGIGVSIAAVIVMPWLAITKRRIAKRIESGALRGDAASSLTCG
                     YMAATVLVGLALNTLFHWWWAEDIAAFVFLFWLVQETLEAFEEARSGKEEGNDD"
BASE COUNT         1326 a         1656 c         1715 g         1280 t
ORIGIN      
        1 gctgctagtc gctcgtctcg gcgttatcgt agtggcgcac ccttgcggtc gccatgcttt
       61 atgcgaatgt ctttggaata gccctgcgca aacaacggct cacattgcaa ttgcagcggc
      121 aggaatctac aatagagggg ataccacccc tgacatccat caaacaacca tagaaagagt
      181 gacgagccga tgaaagcagt acaactcgcg caatatggcg gaccagaagt tttgcaggtg
      241 caggaagtcc ccgatcccgt tcctggcccg caggatgtgc tggtcaaggt cgcagccacc
      301 accgtcaacc ggctggattt atttcaacgc gacggcagca gaccgaccgg caagctgccc
      361 tttacgccgg gattagaggc cgttggaacc gtgcttgccg acaacgcggg ctttcgcgct
      421 ggcgagcgcg tcatgaccac ctgggcgcag gcagccaaag gcggtggcgg ctacgccggt
      481 atcatcgcgg cgacggcgtc caccctgata cgcatccccg ataatgtctc gtttgaggaa
      541 gctgtcgcca ccggtctcgt ttcgtccacc gcctggggcg cattatttga cgtgggcggc
      601 ttgcaacgcg gcgaaagcgt gctgatctgg gccggttcaa gcggcgtcgg caccatcgcc
      661 atccagatgg ccaaacacgc gggctgcaag gtcttcacaa cggccagcag cgttgagaaa
      721 gcggcgaagc tgcgcgagct tggcgcggat gtcgtagtta accacaaaac cgaggatgtg
      781 gcacaggtcg tgcgcagcgc gggcggcgtc aacctggtga tcgaactggt cggcacaacg
      841 ctgcaaacca gcatcgatgc ctgcgccaac ggcggtcgcg tcgtgctgat cggcaacctg
      901 ggcgggcaga acgcgaccgt agacacgcaa tcctggcggc tcaaactggt caacgtgctg
      961 ggcgcgggca tccgccacac ctcggtggag aacgagcggc gcatcctgga actggtcgcg
     1021 gaaaaggcca ttcgccccgt catcgggctg accttgctgg tcgagcaggc tgccgaggcg
     1081 catcacatcc tggcgaaaaa cgaaatcatg ggcaaaatcg tgctgatgca cgaaaagccg
     1141 taggaaacta ccatgagcgc acacaaattt cgcacattcc tccccctctt cgacgaactg
     1201 cgaggggaac gagtcattgt gcggccttac cgcgaaagcg acgcgcaggc gctccaggag
     1261 gcggtggcgg aatcgcgcga caacctgcgc ccctggatgc cgttcgccga tgaacaccag
     1321 accgtcgatg aaagccgcga ttggattatc caccagatag ccggctggct gacgcgcgaa
     1381 agcctggtag tagggttctg ggatgcagcc acgcagcgct acctcggcgg cagcggctat
     1441 cacgtgcgtg actgggagat cggctactac gaaatcggct actggatacg tgtcaccgcc
     1501 gaaggtcacg gctacgtcac cgaaacgata cgcctgctca ccgattacgc ctttgaaacg
     1561 ctcaaagcca accggctcga aattcgctgc gatgagctga acgtgcgcag cgccgccatc
     1621 ccacgacgcc tgggctacat tgaagagggt ctgttgcgca acgacaccat cacccctggc
     1681 ggcgacctgc gcaacacgct ggttttttcg ctcatcacag gcgaatggcc aagagagaga
     1741 taatagattt aacgatcgcg gcaacccgta ggtgctgatg atggacattg acaaattatc
     1801 ccggccaccg gagcagggaa aatgagggcg accgcaaggg tctccacccc accccgctcc
     1861 gctccacccc cgcccttaca atgaacggcg agtggcgtgt cgagcaggct tcggagtctt
     1921 tgtaagggcg ggggtggagc ggagtggggt ggagaccctt gcggtcgccc tggggcttcc
     1981 cgatccagtg gccgccttat ttcgttaatc tgcatgatcg gcctggatgc ccactgttca
     2041 cacctatcga ggccgataaa tcggcggtgt gcgcggtcaa ccggcaccta cagagattta
     2101 tatccgctct actaaaggag aactaatatg tcccgtattc gttggagggt cgtcagcctt
     2161 gtattgctcc tgagttccct gttgctggcg ctcttcctct cgcccatctt tcactcgcaa
     2221 gcaagcgcga gcctggtgca actcagcagc gatccttaca ccaacactac gaccaagcac
     2281 agcaccgagg tcagcgatgc cagctattct tatggctcga ccatcgtgtc cgcgttccag
     2341 gttgggaagt cctacaatag cggtggcgca tccaatatcg ggtgggcaac ctctacagat
     2401 agtggggcga cctggacaaa tggctttctt cccaatatca caaaggtggc ggggggtccc
     2461 taccagcgag ccggggatac tggggtgact tacgatgccg cgcatggcac ctggatgatt
     2521 gttaccaatg gtttttataa tgaagcgttc gctattctcg tcagcctctc ttccgacgga
     2581 aagaattgga gcaatcctgt cgctgttatc gccgttccca agtcgaatac aaacgacttc
     2641 gataaaagcg gcattgcgtg tgataacacc tccacaagcc cgtactatgg tcactgttac
     2701 gtaaactggg tgaacgaagc taaccatgac ctgattgcaa tgagtacttc caccgatggc
     2761 ggtctgacat ggagcgcgcc actgcctacc gccgactcag ccgatggctt caacgggcat
     2821 ccattcgtgc agcccgatgg cacggttgtc gtcgtgatca ataagcgcat cttcgcgccg
     2881 ccgcagacca cgcacatctt ggctttcacc tcgaccgatg gcggcgcgag ctggagcagc
     2941 acggtcacgg tcgtggcaaa atcccagaat catcccaata gcggaggcat acgcgatctc
     3001 cactatttca ccgccgggat ggatggcgcg ggcaagctct acgtggtctg gaatgactgc
     3061 cgctacgaga gtggctgcac ggcgaatgac ctgttgatga tcacctcaac gaatgggaca
     3121 acgtggtcgg ctccgcagcg tatcccaatc gaatcggtgg gcagcggcgt ggatcatttc
     3181 attcctgggc tgggtgttga cgcatctact tctggcagca cagcccatct tggcctggct
     3241 tattactact atccagttgc caattgcacc tcagcgacct gccaggttga cgtaggctac
     3301 gtctcatcca cagatggtgg aaatacctgg tcatcgcctg cacaactgga cgggccaatg
     3361 agtgtgacct ggttcgtcaa tacgcaatgg ggctacatgc ccggtgacta tatcaccacg
     3421 tccttcgctg gcggcaaggc atttcccgtg ttttcggtcg ctagcgcgcc gaatggcagc
     3481 acgtatgacc agaccacgga cacggtggta aacggcctat agaaataagt gaaggaggca
     3541 acctcctgcc cccttcgccc ttccttagag tttggagaat gagcttgaag gaaaagtgat
     3601 taggctgatg aaatatcgtt caggtctttc tcgaagaaat gtcgcgatag gtagtctaga
     3661 attggcgatc atccttttcg ctcttgtaat cttgattgcg ccccaggttc cttctcgtgc
     3721 cgctggcaca ggcgatgtac ccacctacat gggcagtaat acgcgtgatg gctactaccc
     3781 caatgagaca ggcatcaatc ctggcaacgc ttctcgcctg gctatcaagt ggaagttgca
     3841 ggctggtgga agtatcgacg tacagcctgt gatcctcaat ggcatcatct attgggggtc
     3901 ctgggatggc tacgaacatg ccacacgcgc aagtgatggt gccgagatat ggaaaaccaa
     3961 catcggcaag actatttcgg caggcacctg cacccccaaa aatgcgggtc cggcaggcac
     4021 ggcgaccatc gtccagatgg cggtcaatgg ggctactgag gtcgtcttcg tccctggagg
     4081 cgatacggct ttctatgccc tggatgccgc aagtggtcat atcctgtgga aaacatccct
     4141 gggcgcatca cccaatgaga tgctctgggg cggatcggct gtctacaatg gtaacgtgta
     4201 taccggcatc tcctcctacg gtgattgtcc gctgacacct gggcatgtct ttgaactcag
     4261 tgggacaacg ggagccatcc tgcaccaatt caataccaca gcccacaact gtcccggtgc
     4321 cggtgcctgg gatgcaccga ccatcgatga aacggccggt acgcttttta tcacgaccgg
     4381 tacaattgcc tcgtgcaacc catctgagat ctacgcctat gctcttctag aactccgagc
     4441 gtccgacctt gctctgctcg gacactggca aattccgaaa agccagcaga cgggcgatgg
     4501 agactttggt agcacagcta ctctcttcac cgctacaatc aacggcacac tgcatcagat
     4561 gattggcacc gtagacaaaa acggcatcta ttatgcattc gatcgcaacg acatcagcgc
     4621 ggggccactc tgggagcgcg ttgtcggcat gattaagaac cacgacaaaa gtagtcgcgc
     4681 gtcgtctgcc tgggatggaa gtaccctcta cgagggcaac acaaccacca caatcaacgg
     4741 gcagagttgt aagggcagtg tgcgcgctat caatccagcc aatggggcct tcctctggga
     4801 atattgcgct ccggggaaag tgactgcggg actgacggta gtacctggac tggtaatcgt
     4861 gggtgcgtat agcgaccttg tcgtgctgaa tgccgccaca ggcaagaaac tgtttttgta
     4921 ccacgatcca tcagcgctcg ccgagttctg ggggacggtg actgtctcga acggcgtggt
     4981 gtatgtcggc aaccgggacg gggccttgta tgcctttgtg gtagtgtgaa ttccctaaaa
     5041 gtatagagtt aacccctgca atgtgctaac atattcctgt ctggttatat gtaaatagga
     5101 aattgctatg agcgcaccat caacgccatc agccgcttta catgcctcgt tctttcgtgg
     5161 tctcgccggc acaacacgac tttcgtgtgt attggcagtg cgcgaaaggg aaggtttgga
     5221 gcttgcccga tgtatggaga aaaggaaacg ccatgagcgt gaatgtgagt cgagcgatag
     5281 aagttcgtag cggtatccgc atcgaagtct ttacagtgat ctggatggtg attgaggcgg
     5341 ctgtttctat tggcgcagga attatcgcgg gaagtacact gctgactgcc ttcggtctgg
     5401 atagcgtgat tgaactggtc agcggcacca ttctgctgtg gcgactgctg atagaggcaa
     5461 gggaaggcga tactgaacgg gttgaacgtg cagaacatcg agcagcctgg attgttgctc
     5521 tttcacttgc ccttttgtgc gcctatgttt tcgggacagc tctctatggc ctgcttactc
     5581 attcaatgcc ggagagttct ccagttggga ttggcgtctc tatcgctgcc gtgattgtga
     5641 tgccctggtt agccatcaca aaacgacgca ttgccaaacg gattgagagc ggagcgttgc
     5701 gaggggatgc ggcaagctcg ttgacgtgtg gttacatggc agccacagtg ttagtcggac
     5761 ttgcactcaa cacccttttc cattggtggt gggctgagga tatcgctgca tttgtctttc
     5821 tcttctggtt ggttcaggaa acgcttgagg catttgaaga agcgcgttcg gggaaagagg
     5881 aaggcaacga tgattaacag ctggtcatgg cttattgaaa taacgaggca ggggttatac
     5941 ccctacctcg ttatcgaagg ctgtacaaaa cgtgtca
//
LOCUS       DASZAK010000839         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_56105, whole genome shotgun sequence.
ACCESSION   DASZAK010000839 DASZAK010000000
VERSION     DASZAK010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56105"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VGT44_14800"
     CDS             <1..499
                     /locus_tag="VGT44_14800"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV2582122.1"
                     /translation="LSVIPERRLIVLLSKQKVTFVDENVRQLIEESIQARRLDLERPV
                     IVQLLANVRVPRVLLQPTRSNILLRDDETCQYCGKRGRELTLDHIIPRSRGGLGTWEN
                     LVACCKSCNGKKGNRLLKEVNMRLLRPPKPLTHEYAGFFLLRYPKLRQAYEEFLMTAH
                     SGIGA"
     gene            611..2107
                     /locus_tag="VGT44_14805"
     CDS             611..2107
                     /locus_tag="VGT44_14805"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEV2582123.1"
                     /translation="MLDLTLAGKIDKGTQNGPAYLCLHGHFYQPPRDDPFTGQIPIEA
                     TATPFANYNEKITAECYRPNAEAGNFDIMSFDLGPTLAAWLEEAHPDVYQRIIEADQK
                     HMARYGVGNAMAQAYNHTILPLATSRDKHMQIAWGLSDFRHRFGREAKGMWLAETAID
                     IETLDLLAQHGVTYTVLAPWQAAEPVDTTEPYIVPLRDGRSITVFFFNAPLSGGVSFD
                     WNTTSNADMFAASYLPRHLVQSKRDAGEAQLILIATDGELYGHHRPWRDKFLTHLITT
                     GAPAYGFEICALEQYMRLYPATKEATLRLPSAWSCGHGVARWDTGCECTEGDSSWKGA
                     LNQALRNLASHNNRLFEQYAGETLRDPWAALDAYPPVREGWESIDSFWARHGTDQHPL
                     KIQRTLLLLEAQYYGQCSFTSCGFFFEDLDRIEPRNNIAFARRAISLIWQALGIDLQA
                     DFVRDLQSVKSWRVPTLTGAEVYRQLPVTRPEQLPELAPIDDTTPPSS"
BASE COUNT          462 a          662 c          634 g          439 t
ORIGIN      
        1 tctttcagtc attcctgaac gtcgtctcat cgtgctactt tccaagcaaa aagtgacctt
       61 tgttgatgag aacgtgcgtc agttgattga agagagcatt caggcccgcc gtctcgacct
      121 ggagcggccg gtgattgtgc agttgctggc caatgtgcgc gtaccgcgcg tgttgttgca
      181 gccgacacgc tccaacattc tgttgcgtga tgatgaaacc tgccagtact gcggcaagcg
      241 aggtcgcgaa ctgacgctcg accatattat cccccgctcg cgcggaggac tgggtacctg
      301 ggagaacctg gtggcgtgct gcaagagctg caatggcaag aagggcaacc ggttgctcaa
      361 agaggtcaac atgcgcctgt tgcgcccgcc gaagccgctc acgcacgaat atgccggctt
      421 cttcttgctg cgctacccca agttgcgcca ggcttacgaa gagttcttga tgaccgcgca
      481 ttccggtatc ggggcgtgaa aaaagaacta gtattgttac aatggataga aaggattcgt
      541 gtattactct ggtagtgctt gttacctatg attggcgatt actgtaagca ttgccaggga
      601 aggattgtct atgcttgatc tcacacttgc tggaaaaatt gataaaggca cgcagaatgg
      661 ccctgcatac ctctgtttgc atggccattt ttatcagccg ccgcgcgatg accccttcac
      721 cgggcaaatc cccatcgagg cgaccgcaac gccttttgcc aactataatg aaaaaattac
      781 ggcggagtgc tatcgtccca acgcggaggc gggcaacttc gacatcatga gcttcgatct
      841 cgggcccacg ctggcggcat ggttagagga agcgcacccg gatgtgtacc agcgcatcat
      901 cgaggccgac cagaagcaca tggcgcgcta tggcgtcggc aatgccatgg cccaggctta
      961 taatcatacc atcctgccgc tggctacctc gcgcgataag cacatgcaaa tcgcctgggg
     1021 gctgagcgac ttccggcatc gctttgggcg agaagcgaag ggcatgtggc tggcggagac
     1081 cgccatcgac atcgagacgc tcgacctgct ggcgcaacat ggcgtcacct acaccgtgct
     1141 tgccccctgg caggcggcgg agccggttga taccaccgaa ccctacatcg taccattgcg
     1201 cgatggtcgc agcatcaccg tcttcttctt caacgcaccg ctctccggcg gcgtctcctt
     1261 cgactggaac acgaccagca acgcagatat gttcgcagcg tcctacctgc cgcgccacct
     1321 ggtgcagagc aaacgcgacg cgggcgaggc gcagttgatc ctgatcgcga ccgatggcga
     1381 actgtatggg catcaccggc cctggcgcga caaatttctc acgcacctga ttaccaccgg
     1441 cgcgcccgcc tatggctttg aaatatgtgc gcttgagcaa tacatgcgtc tctacccggc
     1501 cacaaaagaa gccaccctgc gcctgccgag cgcgtggagc tgcgggcatg gcgtagcgcg
     1561 ctgggatacc ggctgtgaat gcaccgaagg agatagctcc tggaagggcg cactcaacca
     1621 ggccctgcgc aacctggcca gtcataataa ccgtttattc gaacaatacg cgggtgagac
     1681 gctgcgcgac ccctgggccg cgcttgacgc ctacccgccc gtgcgcgagg gttgggagtc
     1741 gatcgatagc ttctgggcgc ggcacggcac cgaccagcac cccttgaaga tacagcgcac
     1801 cctgctgctg ctcgaagcgc aatattacgg gcaatgcagc ttcaccagtt gtggcttctt
     1861 cttcgaggac ctggatcgca tcgagccgcg caacaatatc gccttcgcgc gccgcgccat
     1921 cagcctgatc tggcaggcgc tgggcatcga cctgcaagcc gatttcgtcc gcgacctgca
     1981 atccgttaaa agctggcgcg tccccactct aaccggcgcc gaagtgtacc gccagttgcc
     2041 cgtaacccgc ccagagcagc ttccagagct ggcgcccatt gacgatacaa cgccgccatc
     2101 gtcgtaggga cgaaatgagg cacttttact aattgacctg gtaatcatgt gtagggacag
     2161 ggggaggcgg cgtggagcgg cgggggcctt gcgcctg
//
LOCUS       DASZAK010000840         1776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_377854, whole genome shotgun sequence.
ACCESSION   DASZAK010000840 DASZAK010000000
VERSION     DASZAK010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377854"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1516)
                     /locus_tag="VGT44_14810"
     CDS             complement(62..1516)
                     /locus_tag="VGT44_14810"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEV2582124.1"
                     /translation="MPDNTQVKFVLNEDEMPRAWYNIMADLRNPPAPVLHPGTHQPVT
                     PDDLAPLFPMSLILQEVSTERYIDIPEPIRDVYRQWRPSPLFRARRLEKALDTTAHIY
                     YKYEGVSPVGSHKPNTAVAQAFYNKEAGVRKLSTETGAGQWGSALAMAGAFFGIDVEI
                     FMVKISYQQKPYRRNLMETYGATVHASPTNLTQAGRSILAKDAENMGSLGIAISEAVE
                     VAAMSGGTTKYSLGSVLNHVLMHQTIIGEETLLQLERAGEYPDVVIGCTGGGSNFAGL
                     AFPFVGKKLREGLKTRVMAVEPMACPSLTKGIYAYDFGDTVGMTPLVKMHTLGHDFIP
                     AGIHAGGLRYHGMSPLVSQLHEEGVIEARAYHQNPTFQASVQFARAEGIIPAPESGHA
                     VRAAIDEALAAKAAGDSRVILFNLSGHGHFDMSAYDSYFSGKLQDYEYPAEAVAEAQK
                     RMPQVVQNLHNRQYLARPSSHGRGIDHSTVGLAK"
BASE COUNT          345 a          508 c          555 g          368 t
ORIGIN      
        1 agagaggaaa aacgaaatct aacctgtact aaaaatatac aacactttgt tatttctatg
       61 ttcattttgc cagccctaca gtactatggt caattccgcg cccatggctg gatggacgcg
      121 ccaaatattg acgattatgg aggttctgca ctacctgggg catacgcttt tgcgcttcgg
      181 cgacggcttc tgcggggtac tcgtagtcct ggagcttgcc ggagaagtag gagtcgtagg
      241 cgctcatgtc gaagtggccg tgaccgctca ggttgaagag gatcacgcgc gagtcgccgg
      301 ctgctttggc ggccagcgct tcgtctatgg cggcgcgcac ggcgtggccg gattcggggg
      361 cgggaatgat gccctcggcg cgagcgaact gcacagatgc ctggaaggta ggattctggt
      421 gataggcgcg cgcctcgatc acgccctcct cgtgcagctg cgagactagc ggcgacatgc
      481 cgtgatagcg caggccgccg gcgtggatgc ctgccgggat gaagtcgtgg ccaagcgtgt
      541 gcatcttgac gagcggggtc atgcccaccg tatcgccgaa gtcgtaggcg tagataccct
      601 tcgtcaggct ggggcaggcc atcggttcga cggccatcac gcgcgtcttc aagccttcgc
      661 gcagcttttt cccgacaaag gggaaggcca gtcccgcgaa attactgccg ccgcccgtgc
      721 agccgatcac cacgtcggga tactcacccg cgcgctcaag ctgtaacaac gtctcctcgc
      781 cgatgatggt ctggtgcatc agcacgtggt tgaggacgct gcccagcgag tacttggtcg
      841 tgccgccgct catggccgcg acctcgaccg cctcgctgat ggcgatgccc aggctgccca
      901 tgttttcggc gtctttcgcg aggatgctgc gccctgcctg tgtgaggttg gtcgggctgg
      961 catggacggt cgcgccgtag gtctccatga gattgcgcct gtagggcttc tgctggtagc
     1021 tgatcttgac cataaagatt tcgacatcga tgccgaagaa cgcgcccgcc atggccagcg
     1081 cagaccccca ctgacccgcg cccgtctcgg tcgagagctt cctcacgccc gcctctttgt
     1141 tgtagaacgc ctgggccacc gccgtgttgg gtttatgact gccgacagga ctgacgccct
     1201 cgtacttata gtagatgtgc gcggtggtat ccaacgcctt ctccaggcgg cgcgcgcgga
     1261 acagcgggct gggccgccac tgccggtaga catcgcgaat cggctcaggg atgtcgatgt
     1321 accgctcggt actgacctcc tgcaagatca acgacatcgg aaataggggg gcgagatcat
     1381 ccggcgtgac cggctggtgc gtgccgggat gcagcaccgg cgcgggcgga ttgcgcaggt
     1441 ccgccatgat gttgtaccag gcgcggggca tctcatcttc gttcagtaca aatttgacct
     1501 gggtgttgtc aggcatagga cgctcctctc tttgctgcca ttgcaaagca gaaaaaaata
     1561 aaatctgccc gagcggatag tgtagaaacc gcagagacgc agaaatgagc cagcctacct
     1621 cgtgggaggt gatgagtaca ctgtatgctg aacggttggg aatgtcaagt atgcggttga
     1681 cttaacccag gtaaacacca gcgcatgaag catggcgacc gcaagggatc gccactacga
     1741 taacgcgatg atagttcgcg tgttcacccg ttatcg
//
LOCUS       DASZAK010000841         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_696614, whole genome shotgun sequence.
ACCESSION   DASZAK010000841 DASZAK010000000
VERSION     DASZAK010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696614"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..469)
                     /gene="msrB"
                     /locus_tag="VGT44_14815"
     CDS             complement(56..469)
                     /gene="msrB"
                     /locus_tag="VGT44_14815"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV2582125.1"
                     /translation="MSDMKNQPESYWKEKLTPEQYKVVRQKGTERAFSGALYYNHDEG
                     MYECVACGQPLFSSDAKFESGSGWPSFDRPVDREHVELHEDNSFFMRRTEVVCKNCGA
                     HLGHVFDDGPRETTGQRYCINSLALDFKPKTNEKG"
     gene            complement(705..1652)
                     /locus_tag="VGT44_14820"
     CDS             complement(705..1652)
                     /locus_tag="VGT44_14820"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2582126.1"
                     /translation="MKLNYDNGCQVLSGIIILLHYTIPTEETSMQYAVTLPNMNYDLR
                     TLVELARDAEASGWDGVFVWDCINMDDKATHDPWVMMSAIAATTQRVRIGPMITPLSR
                     RRPWKLARETVTLDHLSHGRVVLPVGLGETNDGGFKNVGEALERKTRAKMLDESLDIL
                     TGLWSGQPFSYDGEHYHVQEMTFMPQPVQSPRIPIWVVGAWPRKKSLARALRYDGILP
                     AKMNEDSSFADMTPDDIRELCAYIDEHRPPSASSTRYDIVYEGETPGDDAVRAADIVQ
                     PLAEAGVTWWLEAVWSKPETEGGAEGMRRRIRQGPPRVG"
     gene            complement(1735..2787)
                     /locus_tag="VGT44_14825"
     CDS             complement(1735..2787)
                     /locus_tag="VGT44_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEV2582127.1"
                     /translation="MALKRELRKINGFGASFFRVAATQIGMAADTDIQVMDILDLAGE
                     VSAGQLADLMGMTTGAIARILNRLEEAGLVRRERDPNDGRRVIVRLERGKDEMPKIGP
                     IFDSLGNAWEELASDYDDEQMAFLLSFLTRLNAMTRKEFIRLQEAPSSEGKIFSAPLE
                     DLSSGRLVVSSWAQLTLRTTEGMADLYQARFEGSVPNVAAKDGVVTIRYSRRLLGLSG
                     EQRQAVVTLSRAIPWQIVIQSGGSDVTAELGGLDLASFEIKGGGSMIHLELPVPSGVV
                     PIRISGGGSEIMVRRPAGVAVRAHLEGWGSACVFDDQTHMGNNGWLQSSGFEPTAPYY
                     NIEVASSGSMVTITSH"
     gene            2973..3098
                     /locus_tag="VGT44_14830"
     CDS             2973..3098
                     /locus_tag="VGT44_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582128.1"
                     /translation="MPMMIETRELSFSFDGAMMDKLTYAALKGLRADVSKVTLLA"
     gene            3162..3923
                     /locus_tag="VGT44_14835"
     CDS             3162..3923
                     /locus_tag="VGT44_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2582129.1"
                     /translation="MQNTAIQVKDIQKSYKKLSVLQGVNFTVERGSIFALLGSNGAGK
                     TTIIKILSTLLKQDGGTAIVNGFDVASQPAHVRQSLSLTGQFAAVDELLTGQENLIMI
                     ARLRHLKQPRQVADELLTRFGLTDAATRRVSTFSGGMRRRLDLAMSLVGTPPILFLDE
                     PTTGLDPEGRLEVWKVVKELADGGRTVFLTTQYLDEAEQLADHIAILHEGKIIASGTL
                     LELQQRFPAAQVEYVEKQPTLEEIFLAIIGKKEEQ"
     gene            3924..4691
                     /locus_tag="VGT44_14840"
     CDS             3924..4691
                     /locus_tag="VGT44_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2582130.1"
                     /translation="METIQNHFFSDLSVMLGRSMRHILRSMDTIITVLITPIAMMLLF
                     VYVFGGAIQTGTANYVNYILPGIVLIAIASGISYTALRLLTDVQRGIMERFRSMPIAP
                     STLLWGHVLTSLVSNAISVVVIMLVALLMGFRSSAGILSWLTIVGILALFTLALTWVA
                     VIPGLTAKSTDGAAAFAYPLIFLPFISSAFVPTRSMPLVVRVFAENQPVTAIVDTIRA
                     LLAGQPVSQEIWIALAWCVGILVVAYIFAMRAYQKRV"
BASE COUNT         1078 a         1392 c         1282 g         1113 t
ORIGIN      
        1 gaccgcaaag gtgcgccctt ctgaccttcg cttacccgcc acaaacgacg cgcacttatc
       61 ctttttcgtt cgtcttcggc ttaaaatcaa gtgcgagtga attgatacaa tagcgctggc
      121 ccgtcgtctc gcgtggacca tcatcgaaga catggccgag gtgcgcgccg caattcttac
      181 acaccacctc ggtgcgcctc ataaagaacg aattgtcctc gtgcagttca acatgctcgc
      241 ggtcaacagg ccggtcaaag ctcggccatc ccgaccccga ctcaaacttc gcatccgagg
      301 agaacaacgg ctgcccacac gccacgcatt cgtacatccc ctcgtcatga ttgtaatata
      361 acgccccact aaaggcgcgt tccgtgcctt tttgacgcac tactttatac tgctcaggag
      421 taagcttttc tttccaataa gattctggct ggtttttcat atcgctcatg atgtgtgcct
      481 ttcaatgaaa tgatgaacta taatccctta accacacatg agaacaaccc ccatcgcgca
      541 tttgttccac gccagagcca atcacaaatc ccggcatggc gaccgcaagg gatcgccact
      601 acgataacgc cggaatgagc ggacatggac gttatcgtag tggcgatccc ttgcggtcgc
      661 catgtcgcta tgtcctcact ttacgcgctg gagttgccat atcctcaccc cacgcgcggc
      721 ggcccctgtc ggatacgcct gcgcatcccc tccgcgccgc cctcagtctc cggcttgctc
      781 cacacagctt cgagccacca cgtcactccg gcttccgcga gcggttgcac gatgtcagca
      841 gcccgcacag catcgtcgcc aggagtttcg ccttcgtaga cgatatcata tcgcgtagat
      901 gatgccgacg gcgggcgatg ttcgtcaata taggcgcaca actcgcggat atcatccggg
      961 gtcatatcgg cgaatgagct gtcttcgttc atcttcgctg gaagaatgcc atcgtaacgc
     1021 agggcgcgcg ccagcgattt cttgcgcggc cacgctccca caacccagat ggggatgcgc
     1081 ggtgactgta ctggttgcgg cataaaggtc atttcctgca cgtggtagtg ttcgccatcg
     1141 tagctgaacg gctggccgct ccacaggccg gtcaggatgt ccaggctttc gtcgagcatc
     1201 ttcgcgcgcg ttttgcgttc cagcgcctcg cccacattct tgaaaccgcc atcgttcgtc
     1261 tcgcccaggc cgacgggcag cacaacgcgg ccatgtgaaa ggtgatccag cgtaaccgtc
     1321 tcgcgcgcca gcttccaggg ccgccgacgc gaaagcggcg taatcatcgg tccgatgcgt
     1381 acacgctgcg tagtggcagc gatggccgac atcataaccc agggatcatg cgtcgctttg
     1441 tcgtccatat taatgcagtc ccagacaaac accccgtccc acccagatgc ctcagcatcc
     1501 cgcgccagct caaccagggt acgtagatca tagttcatgt tgggtagtgt gactgcgtat
     1561 tgcatagatg tctcctctgt tggtatcgta tagtgtagca ggatgattat accagataat
     1621 acctgacatc cattgtcata attaagtttc atgaccaaag cggcgcaatg gagtcaacgc
     1681 attcaaacct gaaaaggaga cgcgacgata tggatgtgct gacccctcaa agcctcaatg
     1741 agaggtgata gtgaccatgc ttccggagct tgcaacttcg atgttgtaat agggggcagt
     1801 cggctcaaag ccagagctct gcaaccaccc gttgttgccc atatgggttt gatcatcgaa
     1861 gacacatgcg gagccccagc cttcgagatg cgctcgcacg gcgacgccag caggccgtcg
     1921 aaccatgatc tctgatcctc ctccactgat ccgtatggga accacgccag agggtacggg
     1981 aagctccagg tggatcatgc tccctccccc cttgatctcg aaggaggcga gatcgagacc
     2041 acccagttcg gcagtgacat ctgatccccc gctctggatc acaatctgcc aggggatggc
     2101 gcggctcagc gtgaccacgg cctgacgttg ctctccactc agacccaaga gccgtcgtga
     2161 atagcggatc gtgaccaccc catctttggc cgcaacgttc ggcaccgatc cctcgaagcg
     2221 ggcctggtag agatcagcca tcccttcggt cgtacgcaag gtcagctggg cccaggagga
     2281 gacaacaagc cggccactag agagatcttc aagcggagcg gagaagattt tcccctcact
     2341 cgatggcgct tcttgcaacc ggataaattc cttcctggtc atcgcgttga ggcgagtgag
     2401 aaacgagagg aggaaggcca tctgctcatc atcgtagtcc gacgccagtt cctcccatgc
     2461 attcccaaga gaatcaaaaa tgggaccaat tttgggcatt tcgtccttgc ctctttcgag
     2521 ccggacaatg actctgcggc catcgttggg gtcacgctcc cggcgcacaa gcccggcttc
     2581 ctccagccgg ttaaggatcc tagcgattgc cccggtagtc atccccataa gatcggcgag
     2641 ttgaccagca gaaacttcac cggcaagatc cagaatatcc atcacctgta tatctgtgtc
     2701 agccgccatc ccgatctgag tcgctgccac ccggaagaag gaagcgccga agccattgat
     2761 cttcctcaat tcccgcttga gtgccacgag aatctcccct cggtttctct gagaacctct
     2821 tgacaatgac tgttcctccg tgtatcctta atttaagtga ctcagtcact taaattaagg
     2881 atacatttta gtgattaaat catattgtac tccatgagta ttcactgatt gggtcagtga
     2941 attgagtata ccatagaaag gtctcatgtg tcatgccgat gatgattgaa acgcgggaat
     3001 tgtctttttc ctttgacggc gcgatgatgg ataagctcac gtatgccgcg ctcaaaggct
     3061 tgagagccga tgtgtcgaag gtgacgcttc tcgcataagg aggaaagatt gtgcaaaaca
     3121 cttcaaatca ggtcgccaat ctccaaaaag gagggaaggt tatgcaaaac actgcgattc
     3181 aggtaaaaga catccagaaa tcgtacaaga agctcagcgt cttacaaggc gtgaacttca
     3241 ccgtggaacg gggcagcatt tttgccctgc ttggctccaa tggcgcgggc aaaaccacga
     3301 ttatcaaaat cctgtccacg ctgctcaaac aggacggcgg aacggccatc gtcaacggct
     3361 ttgatgtggc gtcacagcca gcccacgtgc ggcaatcact cagtctgacc gggcaatttg
     3421 ccgccgtgga tgagctgttg accgggcaag aaaatctgat catgatcgcc aggctgcgac
     3481 atctcaaaca gccgcgccag gtggccgacg agttgctgac ccgcttcggc ttaaccgatg
     3541 ctgccactcg ccgtgtgtcg acgttttcag gcgggatgcg ccgcaggctg gacctcgcca
     3601 tgagcctggt gggaacaccg ccgatcctgt tccttgacga gccgaccacc gggcttgacc
     3661 ccgagggacg cctcgaggtg tggaaagtgg tgaaggagct tgctgatggc ggcagaaccg
     3721 tcttcttgac cacgcagtac ttagatgagg cggaacagct tgctgatcac attgccattc
     3781 tgcatgaggg caagattatc gccagcggca cgctcctgga gctacaacag cggtttccag
     3841 cggctcaggt ggagtatgtg gaaaaacaac cgaccttgga ggagatcttt cttgccatca
     3901 ttggcaaaaa ggaggaacag taaatggaaa cgatacagaa ccactttttc agcgatctga
     3961 gcgtgatgct tggtcgttcc atgcgccata tcttgcgcag tatggacact atcatcacag
     4021 tcctgatcac gcctattgcg atgatgttgc tgttcgtgta tgtgttcggc ggcgccattc
     4081 aaaccgggac cgcgaattat gtcaattaca tattacctgg gatcgtactg attgcgattg
     4141 cgagcgggat ctcctatacg gctttgcgtt tgttgaccga tgtacaacgg gggatcatgg
     4201 agcgatttcg ctccatgccg attgccccgt ccaccctgct gtggggacac gtcctgacct
     4261 cgctggtctc caacgcgatt tcggtggtgg tcatcatgct cgtcgcattg ctgatgggct
     4321 ttcgttcgtc ggcgggcatc ctgtcatggc tgaccatagt cggcatcctc gcgttgttta
     4381 cgctggcgtt aacctgggtg gcggtgattc ccggactgac cgccaaatcg accgacggcg
     4441 cagccgcctt tgcgtatccg ctgatcttcc tgccctttat cagctcggcg tttgtgccaa
     4501 ccaggtcgat gccgctggtc gtcagggtct ttgccgaaaa ccagccagtg acggcgattg
     4561 tggacaccat ccgtgcgtta ctggctggac agcctgtgtc tcaggaaata tggattgctc
     4621 ttgcatggtg cgtcggcatt ctggtggtag cctacatctt tgcgatgagg gcctatcaaa
     4681 agcgcgtgtg aaaagagggc agtctccgag gaaaggagtt caccacatct ctgctcttga
     4741 tcgacacttg aagcacgtcc ttatgacaga gagccgacag gatgtacgat agtctgtgat
     4801 gtaaattaca agattcggtg tcgtagtttc gtgatacttg tttgtcgtaa aacaggcgtt
     4861 ctttt
//
LOCUS       DASZAK010000842         2526 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_163805, whole genome shotgun sequence.
ACCESSION   DASZAK010000842 DASZAK010000000
VERSION     DASZAK010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163805"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1083
                     /locus_tag="VGT44_14845"
     CDS             88..1083
                     /locus_tag="VGT44_14845"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEV2582131.1"
                     /translation="MNRLYEDDSEEELITFEKIKPGARERARTRTQAQDKPTRKSSYE
                     SSADVQRWLREQSYDAQQLSPGFHPTLLASRRDAPWILSSLEPFYKQHLITDVLHEAH
                     SGKEATVYCCQAHPSTGMEYLAAKVYRPRMFRSLRNDAVYRYSRVQRDEQGQAEHGNS
                     RRGSAATRKTEKGRAAQVASWIEYEYQTQRLLYAHEANVPRTFAQIGNAVLMEYIGDV
                     GDPAPRLSDVVLEQEEAQPLFDCIISNIELALIHGRIHGDLSEYNILYWNGAVTLIDF
                     AQAVDPYHNSDVFSLFTRDIERICRYFAVYDVQSNAYELAREIWTGHMGPIPELI"
     gene            1111..1989
                     /locus_tag="VGT44_14850"
     CDS             1111..1989
                     /locus_tag="VGT44_14850"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2582132.1"
                     /translation="MTTSILPIGVTGSTGQLGGRVATRLAALGLPQRLLVRDRACAPL
                     LAGAEIIQASYEDGLSMRAALSGIQILFLVSGYGDTRVEQHYSAIDAARAAGVERIIY
                     TSFLAAAPLATFTHAREHYLTEEHIRASGCRYTFLRPTFYLDRAPRWFSSEGVIQGPA
                     GNGTISWVSRDDLADVAVAVLTTSGHDGASYEITGSRALTLAQAAEEFSRATGLSTSY
                     QPETLEEARASRMKYNPTDWELEAWVSTYVAIATGEMSVVSHTVEALTGHAPQSLADY
                     VRQHPESYQHITGTRL"
     gene            complement(2297..>2526)
                     /locus_tag="VGT44_14855"
     CDS             complement(2297..>2526)
                     /locus_tag="VGT44_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582133.1"
                     /translation="PEAQRIVKALCDRAVSSGIHLRQAALEEVQVHSLLSLEEIDRAL
                     DPGEYLGSTDAFIEMALASYRVVQSLMGGSG"
BASE COUNT          567 a          734 c          672 g          553 t
ORIGIN      
        1 gcatctgaaa ggaatgagct atgcaacaga gagagaagca aacactcttg aaagtacagc
       61 atgttttcac ctataggagc tattaccttg aatagacttt atgaagatga ttccgaagaa
      121 gagcttatca catttgaaaa aataaagccc ggtgcgcgcg agcgtgccag aactcgaact
      181 caagcgcagg acaaacccac caggaagagc agttatgaaa gcagcgccga tgtccagcgc
      241 tggttgcgcg aacagtcata tgatgcgcag cagcttagcc ccggcttcca tccaacgttg
      301 ctggcatcca ggcgcgacgc tccctggatt ctttcctcgt tggagccttt ctataaacag
      361 catttgatca cggatgtgct gcacgaagct cacagcggca aggaggccac ggtctactgc
      421 tgccaggccc atccatcgac ggggatggaa taccttgcag caaaggtata ccgcccgcgt
      481 atgttccgca gcctgcgcaa cgatgccgtc tatcgctata gccgggtcca gcgcgatgag
      541 caggggcagg cagagcacgg caacagccgc cgtggcagtg ccgcgacacg caagaccgag
      601 aaagggcgcg cagcccaggt agcgtcctgg atcgagtacg agtaccagac gcagcgcctg
      661 ctctacgccc atgaagccaa cgttcctcgc actttcgcgc aaatcgggaa tgctgtcctg
      721 atggaataca ttggggatgt aggcgatccg gctccccggc taagcgatgt cgtacttgaa
      781 caggaggaag cgcaaccgct ctttgattgt atcataagca acatagagtt ggctctgatt
      841 cacggtcgca ttcacggaga cctgtcggaa tacaacatcc tgtactggaa tggagctgtg
      901 acgttgattg actttgcgca ggcggtcgat ccttatcaca actcagatgt tttctcgttg
      961 ttcacgcgtg atattgagcg catctgccgg tacttcgcgg tttatgatgt gcaaagcaac
     1021 gcgtatgaac tcgcgcgaga gatatggacc gggcacatgg gaccaatccc tgaactgatt
     1081 taagctcaat cgccctgaaa ggaacgtttc atgacaactt ccatcctccc cattggggtg
     1141 accggctcaa ctggacaact cggaggtagg gtggcgaccc gactcgccgc tcttggcctg
     1201 ccccagcgcc tgctcgtcag ggaccgcgcg tgtgccccac tgcttgctgg cgctgagatc
     1261 atccaggctt cctatgaaga tgggctatcc atgagggcag ccctcagtgg catccagatc
     1321 ctgtttctgg tttcaggcta tggggacacc cgcgtggagc agcactacag cgctatcgat
     1381 gctgctagag ctgccggtgt cgagcgcatc atctatacct ctttcctggc tgccgctccg
     1441 ctggcgactt tcactcatgc ccgcgaacac taccttacag aggaacatat ccgtgcatcc
     1501 gggtgtcgtt atacgttcct gcgcccgacc ttctacctgg atcgggcgcc tcgctggttc
     1561 tccagtgagg gggtcatcca gggtcctgct ggcaacggca ccattagctg ggtctctcgc
     1621 gacgacctgg cagatgtggc cgtcgcagtc ctgaccacca gcgggcacga tggggccagc
     1681 tatgaaatca ccggttcccg agcacttaca ctcgcacagg ctgctgaaga gttctcacgc
     1741 gccacgggtc tctcgacatc ttatcagccc gaaacccttg aggaggccag agcgtcccgc
     1801 atgaagtaca atcctacgga ctgggagctt gaggcctggg taagcaccta tgtcgccatc
     1861 gccacgggtg agatgtccgt cgtcagtcac acagtggagg cgctcaccgg tcatgccccc
     1921 cagtccctgg ctgactatgt ccgccagcat ccagagagct accagcacat caccgggacc
     1981 cggctttgac ctcgttcaaa ataaggtgaa tcaacgcatt tacaactttt catcactttt
     2041 tccatctaac atattatatc gctcagatac cgctcaaaat gcgcgctact ttttagcaca
     2101 accattgtgt ttttctgtgc gtgctgtagt ttgcttgcca gcacttgttg aacccggata
     2161 ccgaaatgtg catcacttgc aaaaagctca tcaatactca tctgtagcgc atctagctat
     2221 ccacgacact aacatgatgc tcctaggttt ttccgtcgag gattgcccgc ccttgtccag
     2281 cggtatacgg cttgtcctat ccactaccac ccatcaacga ctggactacg cgataggacg
     2341 cgagggccat ttcaatgaat gcatccgtac tgcccaggta ttcgccaggg tcgagggccc
     2401 tatcgatctc ttcaagggag aggagggaat gcacttgcac ctcctccagg gcggcctggc
     2461 ggagatgtat gcctgaactg acggcgcgat cgcagagagc cttgacaatg cgctgtgcct
     2521 ctggtc
//
LOCUS       DASZAK010000843         6737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_234438, whole genome shotgun sequence.
ACCESSION   DASZAK010000843 DASZAK010000000
VERSION     DASZAK010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234438"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..945
                     /locus_tag="VGT44_14860"
     CDS             67..945
                     /locus_tag="VGT44_14860"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEV2582134.1"
                     /translation="MREPARIPVARRILMLLVLVLAWALAACAGQQSPPAGTATQDPP
                     VPQLTQATHGIMDTTGTVPQSLLEQLRTRSDEVQAHGYQIAVVFFNNLASDPHQFATQ
                     VFNTNGIGSKDKNNGVLLVLYLQKPGTDGHAPWLSYITGGGISTALPDTLMDDYVQAT
                     YVPARAQGNWQDGLLAFYDKIYTAEQDPSIAQQWQSQHPQSPAATGPSNNLVQTIGIL
                     ILVCLAIVFWLVVSAIVAARRRENFVYTAFRLLGRFLFVLVLLSRGMGGGSRSRSGGS
                     GPWGGGGSSPSSGGDV"
     gene            974..1693
                     /locus_tag="VGT44_14865"
     CDS             974..1693
                     /locus_tag="VGT44_14865"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV2582135.1"
                     /translation="MFDNEKLPTYDDRTGYGERPGYARESPAYSGMTTAGMGEKAASP
                     KPFWTRAKIISAAVIALLLLGGIVCIYQNITIQQRQYDIQGKVGAVWNEVDRASQLLQ
                     QDAALLNSTIASQNEAISKIAQARSNVASIKAGQGTSDPNAALAAANQLNAGIRVFVE
                     SYPDYGVGNNTHSVLVQIEGSFNRIEYARSQLISAQTDFNKWLLTQIPMNLFYHPVQI
                     LGSNSNPAGPVPVPTYAQPTP"
     gene            1765..2910
                     /locus_tag="VGT44_14870"
     CDS             1765..2910
                     /locus_tag="VGT44_14870"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEV2582136.1"
                     /translation="MAIKSDIERYRANFRAEQEGALLYLELAKAEADTHLAELYRRMA
                     ETEQRHAAVWAVRLRGAGQPEPTYLPGWRIRALVWLAKRFGVGAVLPMVSTMERDASR
                     AYDEQPEARAAGMPAQERSHARLFQAIQSSAPGGIAGPVLARFEGRHRSTGGNALRAA
                     VLGASDGLTTNLSLVMGVAGANLPGHTILFTGLAGMLAGALSMSIGEWLSVQSARELY
                     THQIAVERQELADMPQEEQEELALIYQAKGIDQETAEKMAERIIAGESQDGAHAAALD
                     TLAREELGIDPKELGGSAWEASITSFLLFAVGAVIPVIPFTFGSGWLAVLMSLVLGIL
                     GLFIVGVGITLTTGASLLKSGGRQVLLGVAASAITFGLGWLVGGRLG"
     gene            complement(2932..4107)
                     /locus_tag="VGT44_14875"
     CDS             complement(2932..4107)
                     /locus_tag="VGT44_14875"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2582137.1"
                     /translation="MQQLNRQDLTTQADVPAEEPSFAVRTPPGRTARGRRIAAIVGLT
                     VLLAVVFGTGLFSGWVYGTRNAGSLLGSPPTTTNSPVTIAGNSLDAAREAAVEKVRPA
                     VVQITIATASGEGLGSGVILDSRGFIVTNNHVVTGAQRIQVTLFDGASLPAQLVGADP
                     LDDLAVVKVSTTAKLTAATLGDSSKLRVGQEVLAIGNPLGITQTVTSGIISALDRAVS
                     SIPDAIQTDAAINPGNSGGALVDLQGELVGIPTLSAIDPQFQTPANGVGFAIPSNRVR
                     LIAPQLIETGKVTHTGRAVMGVQIASVDAALAAQNNLPVTSGALIVGVTPGSPAAIAG
                     LKAGDIIAQIGTHPVADVASFADALLTMSPGQKIAVHVYRGDRQLTVNVTLGEARVQ"
     gene            complement(4159..5595)
                     /locus_tag="VGT44_14880"
     CDS             complement(4159..5595)
                     /locus_tag="VGT44_14880"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2582138.1"
                     /translation="MSKKSGAVLERATPVVQQSTLSINTSGAAHQHTLRNIGLIAIYE
                     FRKRIRQRSFIVVTIIMLVLVVLGACAPTAIQYFTATSSSQTKMVVVNNAGSIAGMND
                     DALSRYIGTALNGTATQTPGAKTAVPPPFAIRTVPASSSISDLQKQLKDGAVSILLVL
                     DRAANGEISFTYYTSASGSSLAGDPHLSGVQALAGQLNVLDKAARLGLSPAQTGSLFA
                     PPDFNVVNTGQNSRSVADTVTGYILAYVGILLIFMSVYMYGYGVATGVAEEKSSRIME
                     ILVNAATPFQLMVGKIVGIGAAGLAQMAAFVVVGIGALLLQTPLKALLLSSNAGGLNL
                     DITSASVTILLLLLVYFLLGFLLYATLFAAVGALVKRQEDLQNVVQPVMWLFTIGYLV
                     SFFGIYSPDAAWVKAISYIPFWTPTTMLMRIGTGAVSWWEIALTIALMLVAIYLCAVI
                     SARIYRFGVLMYGQKPGLRQLLRLARRA"
     gene            complement(5630..6565)
                     /locus_tag="VGT44_14885"
     CDS             complement(5630..6565)
                     /locus_tag="VGT44_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2582139.1"
                     /translation="MGLVVEQINKSFVGLRVITDLSMEVQEGSIFGFLGPNGAGKTTT
                     MRMILDILRPDSGHITWNGQDVSAVPRRNWGYLPEERGLYPKVEVEEQLIFLARLYGL
                     SKSSARAHLDEWLERFQIAEYRNKKVEELSKGNQQKIQFLATVLHDPLILIMDEPFSG
                     LDPINATILKEAFEEMHRRGKTIIFSTHQLEQVEELCQDVVIINKGRTIVQGSVREVK
                     RQHGRNVARLKLDNDPEARWLEELAGVQVTRRRQDYIEMQIQANLNPNLIVEEALRRG
                     GVISRFELAEPSLTDIFIEHVNEAARPDAPARLSA"
BASE COUNT         1353 a         1992 c         2040 g         1352 t
ORIGIN      
        1 ggattacgct ttgagctgga gtacaatcaa ggctttacga gaaggaatga gacaaggagg
       61 cgttgtatga gagaaccagc tagaattcct gtggcgaggc gaatcctgat gcttctcgtc
      121 ctcgttttgg cgtgggcgct cgcagcctgc gcgggtcagc aatccccacc cgcggggact
      181 gcgacccagg acccacccgt cccgcaactc acccaggcaa cgcacggcat catggatacg
      241 acgggaaccg tcccgcagag cctccttgag caactgagaa cacggtcaga cgaggtgcag
      301 gcccatgggt atcagattgc ggtggtgttc ttcaataacc tggcgagcga tccgcatcaa
      361 ttcgccacgc aggtcttcaa taccaatggg atcggctcga aagacaagaa taacggcgtc
      421 ctgctcgtcc tgtacctgca aaagccaggc accgatgggc acgctccctg gctgagctac
      481 attaccggcg gcgggatctc taccgcgctg cctgatacgc tgatggacga ctacgtccag
      541 gcaacgtatg taccggcccg cgcgcaaggc aactggcagg atggcttgct ggcgttctac
      601 gacaagatct acacagctga gcaagatccg agcatcgccc agcaatggca gagccagcat
      661 ccacaatcac cggctgcaac cggtccttcc aataatcttg ttcagacgat aggaatactc
      721 atcctggtgt gtctcgctat cgtcttctgg ctcgtggtga gcgctatcgt cgctgcacgc
      781 cggcgagaga attttgtcta cacggcgttc cgcctgctcg ggcgattctt gtttgtgctt
      841 gtattgctca gccgtggcat gggaggagga tcgcgctctc gtagtggggg gtcaggtccc
      901 tggggaggcg gcggcagctc accgtcatcg ggcggcgatg tgtaacagat ctgaaagaag
      961 gaggaatacg cacatgttcg acaacgaaaa gctcccaacc tacgacgata ggacagggta
     1021 tggcgaacgt ccgggctatg cgcgcgagtc tcccgcttat agcgggatga ccaccgcggg
     1081 tatgggagag aaagcggctt cgccaaagcc tttctggacg cgcgctaaaa tcatctcagc
     1141 ggctgtcatt gcgctcctat tactgggagg catcgtctgc atttaccaga acatcaccat
     1201 ccagcagcgg caatatgaca tccaggggaa ggtcggcgct gtctggaacg aggttgatcg
     1261 cgcatcgcaa ctgctccaac aggacgcggc gctgctcaac tcgacgattg cgtcccagaa
     1321 cgaggccatc agcaagatcg cccaggcgcg cagtaatgtc gcgtcgatca aagctggtca
     1381 gggaacaagc gatcccaacg cggcgctcgc cgcggcaaac cagctgaacg ccgggattcg
     1441 cgtctttgtc gagagctacc cggattatgg ggtaggaaat aatacacact cggtgctggt
     1501 gcagatcgag gggtcgttca atcgcatcga gtatgcacga agtcagctca tcagcgcaca
     1561 gacggacttc aacaagtggc tgctgacgca gatccccatg aacctgttct atcaccccgt
     1621 gcagattttg ggatcgaact ctaatccggc cggacctgtt ccggttccaa cctatgcgca
     1681 gcccacgcca tagccctgat gaggcagcga cgacggagtt gaagcgcaca aggaagtagt
     1741 ccgagtctag gcaaaggaga caacatggcg atcaaaagcg acatcgagcg ctaccgggcg
     1801 aatttccggg ccgaacagga gggtgcgtta ctctacctgg agttggcaaa ggctgaagcg
     1861 gatactcacc tggctgagtt gtaccgccgc atggccgaaa ccgagcagcg ccacgcggcg
     1921 gtgtgggccg tacgcctgcg tggcgccggt caaccagagc caacctatct gccaggctgg
     1981 cgcatccgcg cgctcgtctg gctggcgaag cgctttgggg tcggtgcggt gctgcccatg
     2041 gtctccacga tggaacgcga cgccagccgc gcatacgatg agcagccgga agcgcgcgcc
     2101 gccgggatgc ccgcgcagga gcgttcgcac gcacgtcttt ttcaagcgat acaatcgtct
     2161 gcgccgggag ggatcgccgg gccggtgctg gcgcgcttcg agggcaggca ccgcagcacc
     2221 gggggaaacg ccctgcgcgc tgccgtcctg ggagcgagcg atgggctgac gacgaatctg
     2281 agcctggtga tgggcgtggc cggggctaac ctacccggac acacgatcct gttcacgggc
     2341 ctggcgggca tgctggccgg ggcgctttcg atgtcgatcg gcgagtggct ctccgtgcag
     2401 agcgcccgcg agctgtacac ccaccagatc gccgtggagc ggcaggaact ggccgacatg
     2461 ccgcaggagg aacaggagga actggcgctg atttaccagg ccaagggcat cgaccaggaa
     2521 accgccgaga aaatggctga acgcatcatc gcgggggaga gccaggatgg cgcgcacgca
     2581 gctgcgctgg ataccctggc gcgcgaggaa ctgggtatcg accccaaaga actgggcggt
     2641 tctgcctggg aagcatccat tacctcgttc ctgctctttg ccgtgggagc cgtcatcccg
     2701 gtgattccat ttacattcgg cagcgggtgg ctggcggtgc tgatgagtct ggtcctcggc
     2761 atcctgggcc tgttcatcgt cggcgtaggc atcactttga caaccggtgc gtctctgctg
     2821 aaatcaggcg gtcggcaggt gttgctgggg gtagcagctt ctgcgatcac tttcggctta
     2881 ggctggctgg ttggcggtcg cctggggtaa caggggcgca tatcaccctc cttattgaac
     2941 tctggcctcg ccgagcgtga cattgaccgt cagttgccgg tccccccggt agacgtggac
     3001 tgcaatcttc tgtcccgggc tcatcgtgag aagcgcgtcg gcaaacgacg ccacatccgc
     3061 caccgggtgc gtcccgatct gcgcaatgat atcacctgct ttgaggcctg ctatcgccgc
     3121 cgggctgccg ggcgtaaccc cgacgatcag cgcgccagag gtcacgggca ggttgttctg
     3181 cgcggccagc gctgcatcta cgctcgcgat ctgtacgccc ataacggccc gtcccgtgtg
     3241 cgtcactttg ccggtctcga tgagctgcgg agcgatcaat cgtacgcgat tggagggaat
     3301 ggcaaagccg acgccgttgg cgggcgtctg aaactgaggg tcgatggccg agagcgtggg
     3361 gatgcccacc agttcgcctt gcaggtccac cagcgcccca ccgctgttgc cggggttgat
     3421 cgccgcatct gtctggatcg catcaggaat cgacgacaca gcccgatcca gcgcgctgat
     3481 gataccgctg gtcacagtct gcgtgatgcc cagtgggttg ccaatcgcca ggacttcctg
     3541 ccccacacgc aacttcgagg agtctccaag cgtggcggcg gtgagcttcg cggtggttga
     3601 gaccttcact actgcgagat catccagcgg gtcggccccg acgagctggg cgggaagtga
     3661 ggcgccatcg aacagcgtca cctggatgcg ctgggcgcct gtgaccacgt ggttattggt
     3721 gacgataaag ccccggctgt cgaggatgac acccgacccc aaaccctctc cactagcggt
     3781 cgcgatagtg atctggacga ccgcagggcg cactttctct acagccgctt ctcgtgcagc
     3841 gtcgagcgaa ttccccgcga tcgtcaccgg tgagttggtg gtagttggag gagagccgag
     3901 gagactccca gcgttccgcg tgccatagac ccagccggag aacaaaccgg tgccaaagac
     3961 gactgccagg agtacggtca acccaacaat cgctgcgatg cgtcgcccgc gagcggttcg
     4021 tccgggtggt gtccttaccg cgaatgatgg ctcctcagcg ggtacatctg cctgggtagt
     4081 gagatcttgc ctgttcagtt gctgcataag gcatcctccc ttcagaatac atggctccat
     4141 gtggttattg atctttattt atgccctccg tgccagtctg agcagttgcc tcaggcctgg
     4201 cttctgccca tacatcagga ccccgaagcg gtagatgcgc gcagagatca ccgcgcagag
     4261 atagatggcc acgagcatca gcgcgatggt cagggcgatc tcccaccagg acacggcgcc
     4321 ggtgccaatg cgcatgagca tagtggtcgg cgtccagaag gggatgtagg agatcgcttt
     4381 gacccatgcc gcgtcagggc tgtagatgcc gaagaaactc accaggtagc caattgtgaa
     4441 gagccacatg acgggctgca ccacgttctg gagatcttcc tgccgtttga ccagcgcgcc
     4501 cacagcagca aacaaggtcg cgtagagcag gaagcccaaa agaaagtaga cgagcagcag
     4561 cagcagtatc gtgacggaag cgctcgtaat atccaggttc agcccaccag cgttgctgct
     4621 cagcagcagg gcttttaaag gtgtttgcag cagcagcgcg ccgataccca cgacgacgaa
     4681 ggcggccatc tgtgcgagcc ctgccgcgcc gatgcccacg atcttcccga ccatgagttg
     4741 gaacggcgtg gctgcgttca ccaggatttc cataatgcgg ctgctcttct cctcggcgac
     4801 ccctgtcgcc acaccatagc catacatgta gaccgacatg aagatgagca gaatcccgac
     4861 gtatgcgagg atatacccgg tgacggtatc ggccactgaa cggctgttct gcccggtatt
     4921 caccacgtta aagtccggtg gcgcgaacag gctgcccgtt tgggcagggg acaagcccaa
     4981 gcgggccgct ttatcgagta cgttcagttg cccggcgagc gcctgcaccc ctgagagatg
     5041 aggatcaccg gcgaggctgg agccactcgc actcgtgtag taggtaaagc ttatttcccc
     5101 gttggccgcg cggtcaagta cgaggagaat cgagacggcg ccatctttca actgcttttg
     5161 caggtcgctg atactgctgc ttgcgggtac tgtgcgaata gcaaagggag ggggtacggc
     5221 tgtcttcgcg ccgggcgtct gcgtcgccgt tccattgagt gctgtcccga tgtagcgcga
     5281 cagcgcatcg tcgttcatcc cggcgatgga gcctgcgtta ttcaccacta ccatcttcgt
     5341 ctgtgaactc gaggtggccg tgaagtattg gatcgcggtt ggcgcgcatg cgccgagaac
     5401 taccagcacc agcatgatga tggtcacaac gatgaaactg cgctgcctga tgcgtttcct
     5461 gaattcatag atagcgatca ggccgatgtt gcgcagggta tgctgatgag ccgcgccgct
     5521 cgtattgatc gacagagtac tctgctgtac gacaggcgtt gctctttcca gcaccgctcc
     5581 actcttcttt gacatcgtgt tactccttct cgtatcgcat gtgcatccct caggcagaca
     5641 gcctcgctgg tgcatctgga cgcgcggcct cgttgacgtg ttcgatgaag atatccgtca
     5701 gagaaggctc ggccagttca aaacggctga tgacgccacc gcggcgcagc gcctcctcca
     5761 caatcaggtt ggggttgaga tttgcctgga tttgcatctc gatgtaatcc tgcctgcgcc
     5821 tggtgacctg cacccctgca agctcctcca accaacgcgc ctccgggtcg ttatcgagct
     5881 tgagccgcgc tacattgcgt ccgtgctggc gtttgacctc tcgcacactg ccctgcacga
     5941 tggttcgccc cttgttgatg atcaccacgt cctggcacag ttcctccacc tgctccaact
     6001 ggtgcgtcga gaagataatc gttttgcccc ggcggtgcat ctcctcgaag gcttccttca
     6061 ggatagtcgc gttgatgggg tccaggccgc tgaacggctc atccataatc aggatgagcg
     6121 ggtcgtgcaa gacggtggcg aggaattgga tcttctgctg gttgcctttg gagagctctt
     6181 cgaccttctt gttgcgatac tctgcgatct gaaagcgctc aagccactcg tccaggtgtg
     6241 cgcgcgcaga ggacttcgat aacccataga ggcgggccag gaagatcaac tgctcctcta
     6301 cctcgacctt cggatagagt cctctttcct ccggcaggta gccccagttg cggcgaggta
     6361 ccgcgcttac atcctggcca ttccacgtga tatgtccgct atcaggtcgg aggatgtcga
     6421 ggatcatgcg cattgtggtc gttttgcccg cgccgtttgg tccaaggaag ccgaagatcg
     6481 aaccttcttg cacttccatc gataggtccg tgatgacgcg caacccgaca aatgacttgt
     6541 taatctgttc aactaccagt cccatcgtat gaactcctcg ctgtcacgcc tagcgtaacg
     6601 gttgaaacaa tatatggtcg gtctttcgcg tttaactttt cgcatgactg agatgcctgg
     6661 tgagtaagct atgaccactg tactcagccc tccacaggta cgctgccagt ccaaagatca
     6721 ccaggccgag tataaag
//
LOCUS       DASZAK010000844         9311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_553648, whole genome shotgun sequence.
ACCESSION   DASZAK010000844 DASZAK010000000
VERSION     DASZAK010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9311
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553648"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..321
                     /locus_tag="VGT44_14890"
     CDS             82..321
                     /locus_tag="VGT44_14890"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEV2582140.1"
                     /translation="MPPVDNVDRTLDCLKLLCPVPIIKISKAAKELQSGQTLLMLADD
                     PGSKADMIAWAKQTGNELLGIEQEGKVFKFYIRKK"
     gene            476..856
                     /locus_tag="VGT44_14895"
     CDS             476..856
                     /locus_tag="VGT44_14895"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEV2582141.1"
                     /translation="MEEDTKKLLYVQTNGVEAPERSATPFYLAASAAAMDIEVGIYFT
                     VNGPTLLVRGVAEELIPKKDGKGAPLRRFIDQAAELGVKFYVCQPSLDLSNLRYEDLI
                     DGVEMIGGAAFNDLALEADAVISF"
     gene            913..1605
                     /locus_tag="VGT44_14900"
     CDS             913..1605
                     /locus_tag="VGT44_14900"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEV2582142.1"
                     /translation="MAQVTVKHPDVPYEEKERLFTQVTKDIRFHDYLKGCYECGICVA
                     ACPSARFYDFSPRKITQAVAREDVELVYKQMNEDVWNCSQCFSCDRCPRGNSPGGLIT
                     IMREVAVKNGLRSAKEALEGYGRVVYKIMSTGTQVSPDMLQPDAFPDWGPEVRDVSEN
                     LDLWRRALPPETMHTTELAWDVDEKTLTELYLIWHMTGVLDMIKEIDEGLHMILQDVM
                     EEALEEKGFSVS"
     gene            1727..2941
                     /locus_tag="VGT44_14905"
     CDS             1727..2941
                     /locus_tag="VGT44_14905"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEV2582143.1"
                     /translation="MTSDVPIPVTAVTRGTIPQRVFQRDPANAPTEDIREKLFELEAQ
                     GEWIVQRVPDDNAEVMTKYGRTKRIPRQFTWHHKSCGQCGHIPGYSTSIFWLHRQLGM
                     EYNDPRDQTSCTAWNYYASATSNAAAQAAVAVRNFAAAYETSYYPMIHCGTSYGHYKE
                     TREQLVHHPELRKQVRDIMAKLGKPLIIPEEIVHYSEWVHVMRYEIAKRKVFDLSNIT
                     VTVHPACHYYKLVAEDAIYDADLYSGQRTAVVSAVVEALGAKVADYSTWFDCCGFGFR
                     HILVQRDFTRSFATLRKIERMKEEANPDVTLTHDTGCVTTLDKSQFVARAHGYNVGIP
                     VLSEAQFAALAMGAHPYRICQLHWHSVDYRPLLEKMGIDWQARWAEFQDDLERLRRGD
                     IQFLSWEEADAK"
     gene            2931..4019
                     /locus_tag="VGT44_14910"
     CDS             2931..4019
                     /locus_tag="VGT44_14910"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2582144.1"
                     /translation="MPSDTVLIIGGGPGGLYAARSVADLGTPVVLVEKRERLGGTPTA
                     AHYASLTPNLEDAETAINRMISEISDDPLIEILLQTKVSAYSGGPGDFHITFTGKNGE
                     TREMGIGSIIVATGFDHFDPGRETQMYGYYEYEDVLTLVDAEKMLKEKRFVRPSTGEP
                     PKRVCFIQCVGSRDRRLGNEYCSKVCCGVASKEAIEIKELLPDAKVFIFYIDMRMYGF
                     WEDQIYWKAQEEHKVNYIRGIVTEVLRRGDTLIIKGEDTTMGRPMEIPMDVIILSVGM
                     VPSAGTIEIAKVLNLPRERHGFLETVGGPLDTVTTPVPGIFVAGAAAGPKDLEDTISM
                     AGTAAMKAVSTVRRSLRQAAAASMGGGG"
     gene            4024..4788
                     /locus_tag="VGT44_14915"
     CDS             4024..4788
                     /locus_tag="VGT44_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582145.1"
                     /translation="MSASAPDNNEIVGQIVDTPSALEFMREALEKITEEELTAIVAEL
                     RYKSQRFRQLFAQETLPMLDETVFRLLLRSVFSTRRSVTRILEQVNVVTFRDALNDLL
                     HGTKPLQGRFDAFCERLEPLDLSIRCDLAGECLHYWDFERYWLWTRWLWDPARNTGAL
                     PLVTIQEYDLHADTPGSIYMKVGEAIAFVNTVGEAAGFQHGGRGLLGTDVYLACVYVV
                     YLYTMTRMRMTKEFNKVIPQFPELTRRLLGIYKMEV"
     gene            4793..6058
                     /locus_tag="VGT44_14920"
     CDS             4793..6058
                     /locus_tag="VGT44_14920"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEV2582146.1"
                     /translation="MAIEPERIHEKEVAVFEGVDISGHWNRMFEQRVIWDYDTSKLDE
                     ITQIEGAESLGACYQCGKCTPVCPVDHVGDYSPRKIFRKTQMGISLLESPDLWLCTTC
                     MNCLRVCPKEVNMIKIMPAVREQAVLQGNAPTELSQAFENTFRYGNPLGEPARKRADW
                     AKDAGVPVPIMAQIHRPVDILWFVECYPAYHPRGKDAARGLARIFNALGVDYGILGVE
                     EKCSCDSQRLAGEKGLFEMFAEHNIKMFSKYEFKTLVTADPHALNAFKKEYPKYGGEY
                     SVLHYTQFLATMLDRIPFKKSLPYRVTFHDPCYLGRHNGEYEAPRRLLEAIPGIELTE
                     MARCKANGYCCGGGGGGMWLDGFSRNHVSERLSERRVREAVSTGADVLAVCCPYEISR
                     FEDAVKSTGNEGKLIVRDIIELIDESMRD"
     gene            6088..6861
                     /locus_tag="VGT44_14925"
     CDS             6088..6861
                     /locus_tag="VGT44_14925"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEV2582147.1"
                     /translation="MNIIVPVKQVPDLVEELEINSEGTDLERDWLKYKMNEWDESALE
                     EALQLKELHGGTVTVLALATGDVDEMLYTCVARGADRVVKISGDFEEHHDSHTAARAL
                     AAVIRERPYDLILTGVQAVDDLDGQVGVLLATYLGIPHISVVSGIEVEPLTGTATVHQ
                     EYAGGIMATFEVDLPTVLGVQASRAIPRYAPVSRVRQAMKTAQIEEVEATTPAGAGIS
                     VRRMFKPESTEHAEMLEGDADEIVNRIIEIFREHGIWRT"
     gene            6864..7841
                     /locus_tag="VGT44_14930"
     CDS             6864..7841
                     /locus_tag="VGT44_14930"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV2582148.1"
                     /translation="MAEHDVFVIAEHLEGKLSDVTFEMLGKARAIAPVLGGRVVAVLL
                     GYKATELAEQLGAADQVLYIEDAGLRYFTPERAEQALRSLLQNEKPALTMIANTSMGI
                     DLSAALSAALDWPLIAYCSDVQVEDGKIVANSQIYGGKIGVETLSESESTLVSVLAGV
                     FSASEGRSGKPASIQTIFGAANEGRVRFKGLTVPEAGDVDITRETLLVSVGRGIGSKD
                     NIELVEELASNLGAALAASRPIIDSGWLPKQRQVGKSGLSVKPKLYMAVGISGAPEHL
                     QGMKDAELIIAINSDAGAPIFEVADYGIVGDLFDIVPALSERLAEVRTT"
     gene            7855..>9311
                     /locus_tag="VGT44_14935"
     CDS             7855..>9311
                     /locus_tag="VGT44_14935"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV2582149.1"
                     /translation="MMWASTWSLILADGPVQLGRPIFEFFSPILEVTFYTLAAISTLV
                     FVLGVFLKVRKYWRGKPEMRFDHLPGRIGRAVRLVAQNATVGKRDRYAGVGHFMIMWG
                     FITLFLGTVILALDYDLIRPINADWQFYRGDFYYIYKLILDTLGITFLIGLLAMMIRR
                     YAFHLPRLNYKRVDRPEDTYDRRPYKVGDALFVGGLLLIGLTGFLLEGSRIAHDQPAS
                     QVWSPVGILVSKLYLGVGLTPPQLVISHMTLWWVHVTLALTLVAYIPFSKGVHLLVDF
                     VNLAFRNEKAGVALPPIQEEPAPAHPGYRDIRDFNWKNLMDLDACTKCGRCHEACPAR
                     ASGAPLSPRDLILDLRDCADEQLAFKAPLSQRMLPMSNGHNGATRDIKPVAGGVIKAE
                     TLWACTTCRACVEVCPVGIEHVPIIVELRRSLLDEGEMDPKLQEALTNLAKYGNSFGI
                     SDRMRAKWTKDLPFKIKDARKEPVDYLWFVGDFAS"
BASE COUNT         2156 a         2437 c         2660 g         2058 t
ORIGIN      
        1 caacgtttca ggcttccgct cgcaacgatg cgagtagagt gatacatcgt aagtgatcga
       61 atacacaata tggaggtgca tatgccaccg gtagacaacg ttgaccgtac attggactgc
      121 ctgaaactcc tctgccccgt acccatcatt aagatcagca aggcagccaa agaactgcaa
      181 tcaggccaga ccctgctcat gctcgcagat gatcccggct caaaagcgga tatgatcgcc
      241 tgggccaagc agactgggaa tgagttattg ggtatcgaac aagagggcaa agtcttcaaa
      301 ttttatatcc gcaaaaaata gggagtactc cctatgtaaa aaggttcagt acttccaggc
      361 agtgtgttta ttgtgacatg cacaattggc aagcggaggg agttgtttgc aacccatcgc
      421 tgcaacgaag ctagcgcttc tacctacgat cagcagcgat aaaaggatta gccgcatgga
      481 agaagacacc aaaaagttat tgtatgtcca gaccaacggc gttgaagcgc ctgagcgctc
      541 tgcgacccct ttctatctcg ccgcttcagc cgccgccatg gatatcgaag taggcatcta
      601 ttttaccgtc aacggcccta ctttactcgt gcgcggagtt gccgaggaac tcatccccaa
      661 aaaggacggg aaaggtgcgc ccttacgccg gttcatcgac caggcagccg aactcggtgt
      721 caagttttac gtctgtcaac ctagcctgga cctgagcaat ttgcgttacg aggatttgat
      781 tgatggcgtc gagatgatcg gcggcgccgc ttttaacgat ttggcactgg aggccgacgc
      841 agttatttca ttctgattcg tactgccatc agcttttgaa caagtgcctt tggcccaaat
      901 ggaggtgctc cgttggccca ggttacggta aaacacccgg atgtacctta tgaagagaaa
      961 gagcgactct tcacccaggt caccaaagat atccgctttc acgattacct gaaagggtgc
     1021 tatgaatgtg gcatctgcgt ggccgcctgt ccctccgccc gtttctacga cttcagcccg
     1081 cgaaaaatta cccaggctgt ggcccgcgaa gatgtggagc tggtctacaa gcagatgaac
     1141 gaggatgtct ggaactgctc gcaatgcttc tcatgcgatc gttgcccacg tggaaactcc
     1201 cctggtgggc tgattacaat catgcgcgaa gtcgcggtca aaaacggcct gcgctctgct
     1261 aaagaggcgc tagagggcta tggccgcgta gtgtacaaga tcatgtcaac gggaacgcag
     1321 gtttcacccg acatgctgca gcccgatgct ttcccagact ggggaccaga ggtgcgcgat
     1381 gtctcagaga acctggatct ctggcggcgc gcgctgccac ccgagactat gcataccacg
     1441 gaacttgcct gggatgtcga tgaaaagacg ctcacggaac tgtacctgat ctggcatatg
     1501 acgggtgtac tcgacatgat caaggagata gacgaaggcc tgcacatgat tcttcaggat
     1561 gtgatggagg aggccctcga ggagaagggg tttagtgtct cttgaccgtc gttctcctgt
     1621 aatccagtct gactgcgtag cagtttaagc gccagtgtcg ctgactggat ggataggtgc
     1681 cgagacggtt gacgttctca ccggcagaga ccggctggga ggttctatga ccagtgacgt
     1741 tcccataccc gtgaccgctg ttactcgcgg caccattcca caacgtgtct tccagcgtga
     1801 tccggctaat gcgcccacag aggacatccg cgaaaagctt tttgaacttg aagcccaggg
     1861 cgagtggatc gtgcagcgtg tcccggacga taacgccgaa gtaatgacca agtatggacg
     1921 caccaaacgc attccgcgcc agtttacctg gcaccataag agctgtgggc agtgcggtca
     1981 tatccctggc tattcgacct ctattttctg gctgcatcga cagcttggca tggaatataa
     2041 cgatcctcgc gatcagactt cttgcacggc ctggaactac tatgcttcgg caacctcaaa
     2101 cgctgcggcg caggcagccg tggccgtgcg taacttcgcc gccgcgtatg aaaccagcta
     2161 ctacccgatg atccactgcg ggaccagtta cggccactac aaagagacgc gtgagcaact
     2221 cgtgcatcat cctgagttac gcaagcaggt tcgcgatatc atggcgaaac ttggcaagcc
     2281 gctgatcatc ccagaagaga tcgttcacta tagcgagtgg gtgcatgtca tgcgctatga
     2341 aatagcgaag cgcaaggtgt tcgatctgag caacatcacc gtgaccgtgc atccggcctg
     2401 ccactactat aaactcgtgg ctgaggacgc catctatgat gctgatctct atagcgggca
     2461 gcgcacggcg gtcgtcagcg ccgtcgttga ggcgctgggg gcgaaggtgg ccgattactc
     2521 tacctggttc gattgctgtg gctttggctt ccgccacatc ctggtacagc gcgactttac
     2581 gcgctcgttc gccaccctac gtaagatcga acgcatgaaa gaagaagcca accccgacgt
     2641 tacgctcacg cacgataccg gatgcgtcac taccctggat aagagccagt ttgtggcgcg
     2701 agcgcacggt tacaatgtcg ggattcccgt gctctccgag gcacaatttg cggccttagc
     2761 catgggtgcg cacccgtatc gcatctgcca attgcactgg cacagcgtgg attaccggcc
     2821 attgcttgag aagatgggaa tcgattggca agcccgctgg gccgaattcc aggacgacct
     2881 cgagcgattg cgcaggggag acatacaatt tttatcatgg gaggaagctg atgccaagtg
     2941 atactgttct gatcataggt ggtggcccgg gcgggttata tgctgcgcgc agcgtggctg
     3001 acctgggtac tccggttgta ctggtggaga aacgcgagcg tcttggcggt acccccacgg
     3061 cagctcatta cgcttcactc acgcctaacc tggaggatgc tgaaacagcg atcaatcgca
     3121 tgattagcga gatcagcgat gatccactga tcgagattct ccttcagacc aaagtgtcgg
     3181 catatagcgg cggacccggt gattttcaca tcacgtttac cggcaagaat ggcgagacgc
     3241 gcgagatggg tatcggctcg attatcgtag ctaccggctt tgatcatttc gaccctgggc
     3301 gcgaaacgca aatgtatggt tactacgagt atgaagatgt gctcacgctt gtagacgccg
     3361 aaaagatgct gaaagagaag cgctttgtgc gcccatctac cggggaacca cccaaacgcg
     3421 tctgctttat tcaatgcgtt ggctcgcgtg atcgacgatt gggaaacgag tattgttcca
     3481 aggtgtgttg cggagttgct tcaaaagagg ctatagagat caaagaactc ctgcctgacg
     3541 cgaaggtgtt cattttctat atcgatatgc gcatgtatgg cttctgggag gaccagatct
     3601 actggaaggc gcaggaggag cataaagtca actatattcg cggcatcgtg acggaggtgc
     3661 tgcggcgcgg ggatacactt atcattaagg gagaagatac caccatgggg cggccaatgg
     3721 aaattcctat ggatgtgatt attctctccg ttggcatggt gcccagcgcc ggtactatcg
     3781 agatcgccaa agtgctgaat ctgccacggg agcgtcacgg cttcctggaa acggttggtg
     3841 gccctctgga tacggtcacg acgccggtgc ctggcatctt tgtcgccggc gcggcagctg
     3901 gccccaaaga ccttgaagac accatctcga tggcaggaac tgccgccatg aaagccgtga
     3961 gcaccgtgcg acgttcacta cgccaggccg cagcagcatc gatgggaggc ggagggtagt
     4021 acaatgtcgg cctcagcccc ggataacaac gaaatagttg ggcagattgt agacacaccc
     4081 agcgcgctcg aattcatgcg cgaggctctc gagaagatca ctgaggaaga actcacagct
     4141 attgtggcag aactgcgcta caaaagccag cgatttcgac aactgttcgc gcaggaaact
     4201 ctgcctatgt tagatgaaac tgtgttccgg ttgctactac gttcagtatt ctcgacaaga
     4261 cgcagcgtaa ccaggatctt ggaacaggtc aatgtagtca ccttccgcga cgcgcttaac
     4321 gacctgctgc acggaacgaa acccttgcag ggacgattcg acgccttttg cgagcgactg
     4381 gaaccgctgg acctgtcgat acgctgcgat ctcgcggggg agtgtctgca ctactgggat
     4441 ttcgagcggt actggctctg gacgcgctgg ttgtgggatc ccgcgcgcaa taccggcgca
     4501 ctgccactgg tcaccatcca ggaatacgac ttgcacgcag atacccctgg atcgatctat
     4561 atgaaggtgg gagaagcgat tgcgttcgtg aatacagtgg gcgaggcggc cggtttccag
     4621 cacggaggtc gaggactgct cggcactgat gtctatctcg cctgtgtcta tgtcgtctac
     4681 ctgtatacga tgacacggat gcgtatgacc aaggagttca ataaggtaat tccgcagttc
     4741 cccgaactca cccggcgtct gcttggtatc tataaaatgg aggtataaga gtatggctat
     4801 cgaacccgag cgcatccacg agaaagaggt tgcagtcttt gaaggcgtag acatctctgg
     4861 tcactggaac cgcatgttcg agcagcgggt gatctgggat tatgatacct ccaaactcga
     4921 tgagatcacg cagatcgaag gagcagagtc tctgggagct tgctatcagt gcggtaaatg
     4981 cacgcctgtc tgcccagtag atcatgtcgg tgactacagc cctcgtaaaa tcttccgcaa
     5041 aacgcagatg ggtatcagcc tgctcgaaag tccagacctg tggctctgca ccacatgtat
     5101 gaactgcctg cgcgtttgtc caaaagaagt caatatgata aagatcatgc ccgcggtacg
     5161 cgaacaggct gtgctgcaag gcaatgctcc tacggaactt tcgcaggcat tcgagaatac
     5221 gtttcgctac ggcaatccac tgggtgaacc ggctcgcaaa cgtgctgact gggcgaaaga
     5281 tgccggtgta cccgtaccca ttatggcgca gattcaccga cccgtcgata tcctctggtt
     5341 tgtagagtgc tatcccgcct atcatccacg aggtaaggat gccgctcgcg gcctggcacg
     5401 tatcttcaat gcccttggag tggactatgg catcctgggc gttgaagaga agtgtagctg
     5461 cgattcacaa cggctggccg gtgaaaaggg gttattcgaa atgtttgcag aacataatat
     5521 caagatgttc agcaaatatg agttcaagac ccttgttaca gccgatccac atgccctgaa
     5581 cgccttcaaa aaagaatacc ctaaatacgg gggagagtat tcggtactcc attatacgca
     5641 gttcctggcg acaatgctgg accgcatccc ttttaaaaaa agcttaccgt accgcgtaac
     5701 gttccatgat ccctgctacc tgggccggca caacggcgag tatgaagcgc cccgccggct
     5761 cctggaggca atccccggca tcgagttgac cgagatggct cgctgcaagg caaatggcta
     5821 ctgttgcggt ggtggaggtg gtggcatgtg gctggacgga ttttctcgca atcacgtttc
     5881 agagcgactc tccgaaagga gggtgcgaga agcggtcagc acgggcgcgg atgtgctggc
     5941 agtctgttgc ccttacgaga tctcgcgttt cgaagatgct gtgaagtcca cagggaacga
     6001 gggaaaattg attgtacgtg acatcatcga gttgattgat gaatcgatgc gtgactgatt
     6061 acgaatggat agaaggagtt gaccagcatg aacatcatag tcccggtgaa gcaggttccg
     6121 gatctcgtag aagagttgga gatcaatagc gaagggacgg acctcgaacg ggactggctc
     6181 aagtacaaga tgaacgagtg ggatgagagt gcgctcgagg aggcgctcca gctcaaggaa
     6241 ctgcacggtg gcacggtaac ggtgctggcg ctggcgactg gcgatgtaga tgaaatgctc
     6301 tacacatgcg tcgccagggg cgctgaccgc gtggtcaaaa tttccggcga ttttgaagag
     6361 catcatgata gccatactgc cgcgcgcgcg ctggcggcgg tcattcgcga acgaccctac
     6421 gatttgattc ttacaggggt gcaagctgtc gatgacctgg atgggcaagt aggcgtcctg
     6481 ctggctacgt atctgggaat accacatatc agtgtggtga gcggcatcga agtcgagccg
     6541 ctgacaggta cagcaactgt acaccaggaa tacgctggtg gcatcatggc aacttttgag
     6601 gtagacttgc ccaccgtgct gggtgtccag gcatcacgtg ccatcccgcg ttatgccccg
     6661 gtgagccgcg tccgccaggc catgaaaacc gcgcaaatcg aggaggtcga ggcaacaact
     6721 cctgccgggg ctggtatatc cgtgcggcgg atgttcaagc cggaatcgac agagcacgcc
     6781 gagatgctcg agggcgatgc cgatgaaatt gttaaccgca tcatagaaat tttccgcgag
     6841 catggaatct ggaggacttg attatggctg aacatgacgt cttcgtcatc gctgaacatc
     6901 ttgagggtaa actcagcgat gtgacattcg agatgcttgg caaagcgcga gccatcgctc
     6961 cggtgcttgg tggccgggta gttgcagtct tactcggcta taaggcaact gagctagccg
     7021 aacaactggg agctgcagac caggtgcttt atatcgaaga tgccgggctg cgctatttca
     7081 ctccggaacg cgccgagcaa gcacttagat cactgctcca aaatgaaaaa cccgcgctga
     7141 ccatgatcgc caacacctct atgggcatag atctgtcagc cgcgctatca gccgcgctgg
     7201 attggccttt gatcgcttat tgcagcgacg tgcaggtgga agatgggaag atcgtcgcaa
     7261 acagccagat ttatggcggc aagatcggag tggaaaccct gagtgagagc gaatccacgt
     7321 tggtatccgt cctcgcaggt gtattttcgg cctcggaggg acgttccggc aaacccgcct
     7381 ctatccaaac cattttcggc gccgcgaacg agggacgtgt gcgctttaag gggctgactg
     7441 ttcccgaagc gggggacgtg gatattacgc gcgaaacatt actggtcagc gttggtcgtg
     7501 gcatcggctc aaaggataat atcgagctcg tcgaagaact ggcaagcaat cttggcgcag
     7561 cgctcgcggc ttcgcgaccg atcattgata gtggctggct gccgaagcag cgacaggtag
     7621 gtaaatctgg cctgagcgta aagcccaagc tctacatggc ggtgggtatc agcggcgccc
     7681 cggaacactt gcaggggatg aaggatgccg aactgatcat tgccatcaac agcgatgcgg
     7741 gagcacccat cttcgaggta gcagattatg gcattgtagg cgacctcttc gatatcgtac
     7801 cagcgctttc cgaacggctg gccgaggttc gtacaacata aagcggagcg ttctatgatg
     7861 tgggcgagta catggtcatt gattctagcc gacggtccgg ttcaactggg taggcccatt
     7921 tttgagtttt tctcgccgat tctggaagtg actttttaca cgttagcggc tatttccacg
     7981 ctggtctttg tactgggagt ctttttaaaa gtgcggaaat actggcgtgg aaaaccagag
     8041 atgcgttttg accacctgcc gggtcgcata ggccgcgccg tgcggctcgt tgcgcaaaat
     8101 gccactgttg gcaaacgcga tcgctacgca ggggttgggc atttcatgat catgtggggc
     8161 ttcatcaccc tgttcctggg aacggtcatt ctcgcgctgg attacgacct cattcgccca
     8221 atcaatgccg actggcagtt ctatcgtggt gatttctact acatatacaa actgatcctg
     8281 gataccctgg gcatcacctt tctgatcggt ctcctggcaa tgatgatcag gcgctatgct
     8341 ttccacctgc cccgactgaa ctataagcga gtagatcgtc ctgaagacac gtatgatcgg
     8401 cgcccttaca aggttggcga tgccctattc gtgggcggac tcctcctgat cgggctgaca
     8461 ggatttctac tggaagggtc gcgcatcgct catgatcagc ccgcatctca ggtatggtct
     8521 cctgtcggca tccttgtctc aaagctctac cttggggtgg gtctgactcc tccgcaatta
     8581 gtaatcagcc acatgacgct gtggtgggtt cacgtgacac tggcgcttac gcttgtcgcc
     8641 tacatacctt tttccaaagg tgtgcatttg ctcgtggatt tcgtcaatct tgccttccgt
     8701 aacgagaaag ccggtgtggc attgccgcca atacaggagg agccagcgcc agcgcatcca
     8761 ggttaccgcg acattcgcga ctttaactgg aaaaatttaa tggacctgga cgcatgcacc
     8821 aagtgcggcc gctgccatga agcctgccct gcccgcgctt ccggcgcgcc actctcgccc
     8881 cgcgacttga tcctggattt gcgggattgc gcggacgaac agctcgcttt caaggcgccg
     8941 ttgagccagc gcatgcttcc catgagcaac gggcacaacg gcgcgaccag ggatatcaag
     9001 cctgtagcag gaggggtgat caaagcggaa actctctggg catgtaccac ctgccgcgcc
     9061 tgcgttgaag tctgtcccgt aggcattgaa catgtgccga tcatcgtgga gctgcggcgc
     9121 agcctgctcg atgagggtga gatggatccc aaactccagg aggcgcttac gaacctggcg
     9181 aagtacggca attctttcgg tatctccgac cgcatgcgag caaagtggac caaagatctg
     9241 cctttcaaaa tcaaggatgc gcgcaaagaa ccggtcgatt atctgtggtt tgtgggcgac
     9301 tttgcatcct a
//
LOCUS       DASZAK010000845         1752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_234496, whole genome shotgun sequence.
ACCESSION   DASZAK010000845 DASZAK010000000
VERSION     DASZAK010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234496"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..731
                     /locus_tag="VGT44_14940"
     CDS             57..731
                     /locus_tag="VGT44_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582150.1"
                     /translation="MEAAEILERARSGEEAPSNWLVLPLLKRKVQVGILGWAFGIIIG
                     FGLFAFIASVTIPYNFTLGILASIFTIVLLAIVLFIGAGSLWAMIADIKRLSHAENYL
                     LVITPDDFVKQEGDKIVHVPLTYVRHVTARGAPPPERATPGKNTFREMPSAGENAAGF
                     VFGRAFFPQGLRQRRKRMRTPTTLAFLDTRDDQEVIVANDSSYGDPFEIAAYLKQYAA
                     RVQQIV"
     gene            761..1561
                     /locus_tag="VGT44_14945"
     CDS             761..1561
                     /locus_tag="VGT44_14945"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEV2582151.1"
                     /translation="MLLAIDISNTNIKFGLYNSETMKNHWVVSTARQRTTDEYAMVLN
                     DLARHAGYRLSDIDDIILSSVVPPLTPVFQELAARYCGKEAIVLDHTLDLGIRLLVDN
                     PWEIGSDRIATTIAAHALYGGPAIIIAFSTATTFDVISGEGDFLGGAIAPGLLISAEA
                     LSSAASRLYRVDMTPPRAALGKNTIENMQSGIIFGHVGLVQGLIARLRKEIPGATEDK
                     AAGEVKVIAHGGLAPLMAPIIPEIQHVNQYLPLEGLRLAYLRIHEASS"
BASE COUNT          397 a          499 c          475 g          381 t
ORIGIN      
        1 aatctggtat aatcatgtca taactcgttc tcacctggag cagaaagaag gatgtaatgg
       61 aagctgccga gatacttgag agagccaggt cgggcgaaga agccccctct aactggctgg
      121 ttcttccttt attgaaacgc aaagtacagg taggcattct tggctgggct tttggtatta
      181 tcatcggttt tggattgttc gccttcattg cctcggtcac gataccgtat aactttacgc
      241 tgggcatcct ggccagcatc ttcaccatcg tgctgctcgc catcgtcctg ttcatcggag
      301 cggggagctt gtgggccatg atagccgata tcaagcggct gagccacgcg gaaaactacc
      361 tgctcgtgat tacgcccgat gatttcgtga agcaggaagg cgacaaaatc gtgcatgtgc
      421 cgctcaccta tgtgcgccat gtcacggcgc gcggcgcgcc gccgccggaa cgcgcgaccc
      481 ctggcaaaaa caccttcaga gagatgccca gcgcgggaga gaatgcagcc ggtttcgtct
      541 ttggccgcgc gttcttcccg caggggctgc gtcagcgccg caagcgtatg cgcaccccca
      601 ccacgctcgc ctttctcgat acgcgcgatg atcaggaggt cattgtggca aacgacagtt
      661 cctatggaga ccccttcgag attgccgcct acttgaaaca atacgcagcg agagtgcagc
      721 aaatagtcta gaagagcgcc atattacaac ggagtcgtct atgctgctgg caatcgatat
      781 cagcaatacc aacatcaaat ttggtctgta caatagcgag acgatgaaga atcactgggt
      841 ggtttccacc gctcgccaac gcacgacaga tgaatatgcg atggtgctaa acgacctggc
      901 gcgccatgcc gggtaccggt tgagcgacat cgatgacatc attctatcca gcgtggttcc
      961 cccactgacg ccagtttttc aggaactggc cgcgcgctac tgtgggaagg aagcgatcgt
     1021 actggatcac acgctcgacc tgggtatccg gctgctggtt gataacccct gggagatcgg
     1081 ctccgaccgc atcgctacca cgattgccgc gcacgcgctc tatggcgggc ctgccatcat
     1141 catcgccttt agcacggcca ccaccttcga tgtaatctct ggcgagggcg atttcctggg
     1201 cggcgcgatt gctcctggcc tgctcatctc cgccgaggca ttgagcagcg cagcctcgcg
     1261 actctaccgc gtcgatatga cgcccccacg cgccgccctg ggcaagaata cgatagaaaa
     1321 catgcaatcc ggcatcatct tcggacacgt cgggctggtg cagggcctga tcgcgcgcct
     1381 gcgcaaggaa atcccaggcg cgaccgaaga caaagcggct ggcgaagtga aggtcatcgc
     1441 gcatggtggc ctggcgccct tgatggcacc catcatcccg gaaatacaac atgtgaatca
     1501 gtatctgccg ttggaagggt tgcggctggc gtatttgcgg atacacgaag catcatcctg
     1561 accactcgaa ttgccctata attactcacg ctcatttacg cctctgatgc aatattttga
     1621 gtgcaaccaa acatgttaat ctcttgcttt gaatagtttc ctatgttaca ttacctgcac
     1681 aatcatcggc gagaagcaat agatgggcat gaaagggcag ttctcatgca tgacgaagca
     1741 ggcgcaactg ac
//
LOCUS       DASZAK010000846         4086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_413266, whole genome shotgun sequence.
ACCESSION   DASZAK010000846 DASZAK010000000
VERSION     DASZAK010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413266"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1467)
                     /locus_tag="VGT44_14950"
     CDS             complement(10..1467)
                     /locus_tag="VGT44_14950"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEV2582152.1"
                     /translation="MYRVRYVEQTDKLGERVHLSMLEEIYSRELLLRCVQQSERRAQK
                     ARRVRHFTGLAVLWLVLAMGLWSRLSQGRVWDKLTHWLQDGIPAQPQEPAGASAVAYQ
                     RALLGVEPLRQLFEQGTHVLGQEQTPGVWYQGYRLMALDGTLFNLPDTPANEQAFGRS
                     RNQYGKGAYPQARVVLLTECGTHATIGLHIGRYDEGEVHGAFGLLPKLERGMLLLHDA
                     NFFGGAYLEALSAKGIRSLGALASTVALTRRRNLPDGSYLAQLVPNAQAVYPMQRPLW
                     VRIIEYQLTDERLGDPGQVYRLVTTWLNPRSAPAKELIVLYHERWEVENVLDELKTHL
                     RVQQKVLRSQTPEGVRQEIYGLFVVHFAIRALMTQAAIEADLDPDRLSFTEALFQLCE
                     AVADERGEVSPLVQQWRATRLRQRLRRRLLPARFLRLNRRELKQVYHKYKPKKRNGVP
                     PKPFEPEERFEDFVVVLLRPQGSTLLTEVTPMGLI"
     gene            complement(1651..3216)
                     /locus_tag="VGT44_14955"
     CDS             complement(1651..3216)
                     /locus_tag="VGT44_14955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV2582153.1"
                     /translation="MHIVTTDEMRQLEIRADSEYGLTSPILMEHAGKSAAEILAQHIR
                     RQQSINEREFLILVGPGNNGGDGKVMGRYLHQWGGAVSYYEWKEQRLTIKEQEVPPHE
                     TAAKLDECFSRAHYILDALLGTGRSRPLPDSMRNLLARARQERERRENARVVAIDLPT
                     GMNADTGEVDPGTIPSDMTITLACPKQGFFFFPARSYIGELYVGDIGLPPALEEGLQT
                     EMLTSALVKRLLPDRPLESNKGTFGKVMLYCGSPPYPGSAYLAGSAAGRAGAGLVTLA
                     VTELMLPIYASSLHETTFLLLPNERAESYELATTLVNSLEGYRVLLIGPGLGQSSYTR
                     EVILQALEQVRALPEQKRPRLIIDADGLNNLSALERWWMLLPPETIITPHPGEMGRLC
                     GGLKVSGGGIDRLPLARERASEWQVTLVLKGACTIIAAPGGRARINWQANPALATAGT
                     GDVLAGLTAGLLAQQVASGETISAFDAASAAVYLHTAAAELVSTDIGHAGLLASDLLP
                     KIPMAIMKCLLKP"
BASE COUNT          785 a         1192 c         1251 g          858 t
ORIGIN      
        1 caatacattt caaataaggc ccatcggcgt cacctccgtc agcagggtag acccttgggg
       61 tcgcagcagc acgaccacga aatcctcaaa acgctcctct ggctcaaagg gcttcggtgg
      121 gaccccattg cgcttcttgg gtttgtactt gtgatagact tgcttgagtt cgcgccgatt
      181 gaggcgcaag aagcgcgcgg gcagcaagcg gcggcgcagg cgctgtcgca gccgggtggc
      241 acgccactgc tgcacgagag gggatacctc gccgcgctcg tccgccaccg cttcacagag
      301 ctggaacagg gcttccgtga aactcaagcg gtcggggtcc aggtccgcct cgatggcggc
      361 ctgggtcatt aaggcgcgaa tggcgaaatg caccacgaac agcccgtaga tctcctggcg
      421 caccccctcg ggcgtctggg agcgcagcac tttctgctgc acccgcaggt gcgttttgag
      481 ctcatccagc acgttttcca cctcccagcg ttcatgatag agcacaatca actccttggc
      541 cggggcgctg cgcgggttga gccaggtggt caccagccgg tacacttggc cggggtcgcc
      601 cagccgttca tcggtcaact ggtactcgat gatgcgcacc cacaggggcc gctgcatggg
      661 atagacagcc tgcgcattgg gcaccagttg ggccaggtaa ctgccatcgg gcagattgcg
      721 ccgccgcgtg agggccaccg tactggcgag cgcccccagg ctgcggatgc ctttggcgct
      781 gagcgcttcc aaataggcgc ccccgaagaa gttggcatca tgcagcagca acatgccgcg
      841 ttccagtttg ggcagcaggc caaaggctcc atgcacctcc ccttcgtcat agcgcccgat
      901 gtgcaagccg atggtggcat gcgtgccaca ttcggtcagc agcaccacgc gtgcttgcgg
      961 ataggcgccc ttgccgtact gattgcgact gcgcccaaag gcctgctcgt tggccggggt
     1021 gtcgggcaga ttgaacagcg tgccatccag cgccatcagc cgatagccct ggtaccacac
     1081 gccgggggtc tgttcctggc ccagcacatg cgtgccctgc tcgaagagct gccgcagcgg
     1141 ttcgacgccc aacagcgctc gctggtaggc caccgccgag gccccggcgg gctcctgggg
     1201 ctgagccggg atgccgtctt gcagccaatg cgtcagctta tcccacacac gtccttgcga
     1261 gagacggctc cacagcccca tcgccagcac cagccacagc accgccagtc cggtgaagtg
     1321 ccgcacccgc cgcgccttct gggcgcgtcg ctcactctgc tgcacacagc gcagcagtag
     1381 ctcgcggcta tagatctcct ctagcatgct cagatgcacc cgctcgccca acttgtcagt
     1441 ttgctccacg tagcgtactc tatacatcag gggatccttt ctgttcctct acaggctcaa
     1501 tgacttgttc tgtagtatct cagatcagct cccctgttgt ctgctttttc tccctccttt
     1561 tcttatgtga aatgtattgg gccttgcgcc tgtcctggca cgcctctccc cgcttccctg
     1621 tccctacgca gctttcgcga tgacggaata tcagggtttt agcaggcatt tcatgatcgc
     1681 catagggatc tttggcagta ggtccgatgc cagcaggccg gcatggccga tgtcagtgct
     1741 gaccagttcg gcggcggcgg tatgcaggta gacggcggca ctggcggcat cgaaggcgct
     1801 gatggtttcg cctgaagcga cttgctgggc gagcaagccg gcggtcaggc cggccagcac
     1861 gtcgccggta ccagcggtgg ccagcgcggg gttggcctgc cagttgatgc gcgcccggcc
     1921 accgggcgcg gcgatgatgg tacaggcgcc tttcagaacg agcgttacct gccattcgct
     1981 ggctcgctcg cgcgccaggg gtaggcggtc gatccctccg ccggagactt tcaggccgcc
     2041 gcagagccgt cccatttcgc caggatgagg cgtgatgatt gtctctggcg gcagcagcat
     2101 ccaccagcgt tccagggcgg agagattgtt gaggccgtcc gcgtcgatga tgaggcgcgg
     2161 acgtttttgc tctggaagag cgcgcacctg ctccaacgcc tggagaatga cttcgcgcgt
     2221 ataggaggat tgacccagac cgggtccgat cagcaggacg cggtaccctt ccaggctgtt
     2281 gacgagcgtg gtagcaagct cgtagctctc cgcgcgttcg ttgggaagca ggaggaaggt
     2341 ggtctcgtgc agggagctgg cgtagatggg cagcataagc tctgtgactg ccagcgtgac
     2401 gaggcccgcg cccgcgcgcc cggcagcgct gccggccagg taggcggagc cgggataggg
     2461 aggcgaaccg caatagagca tcactttgcc gaaggtgcct ttgttgcttt cgagcgggcg
     2521 atctggcagc agtcgtttca ccagcgcgct ggtcagcatc tcggtctgta gtccttcttc
     2581 cagggcaggc ggcaggccga tatctccaac atacaactct ccgatatagc tgcgtgcggg
     2641 gaagaagaaa aagccttgct tggggcaggc aagcgtgatg gtcatgtccg agggaatggt
     2701 gccgggatcg acttctccgg tgtcggcgtt cataccggtg gggagatcaa ttgcgacgac
     2761 gcgggcgttt tcgcggcgct cgcgttcctg ccgcgcgcgc gccagcagat tgcgcatact
     2821 gtcagggagc gggcgcgagc gccccgttcc gagtagggcg tccaggatat aatgggcgcg
     2881 cgagaagcat tcatccagct tcgcagcggt ttcgtgcggt ggaacctcct gctccttgat
     2941 cgtcaggcgc tgctccttcc attcatagta gctgaccgct cctccccatt gatgcagata
     3001 tcgtcccata accttgccgt cgccgccgtt attgcctggg ccgaccagga tcagaaactc
     3061 gcgttcgttg atcgactgct ggcgtcggat gtgctgcgca agaatctcag ccgcgctctt
     3121 tccggcgtgt tccatcagga ttggggatgt cagaccatac tcgctgtcag ccctaatttc
     3181 gagctggcgc atctcgtcgg tagtaacgat atgcattgtt ttcttctccc aaagtatttt
     3241 gatgcctctg tactgattgt aatgcgaaat cgggcgcgtg ggaagaggct gccaaaaatc
     3301 aatgagacag cgatgagggg gaccatcgct gtctcattgt gggaaggaga tagagaacat
     3361 gatttgagtt catctccacc ggtggattgt tttgtgtggt agtggtggcg atgattgcgg
     3421 cttgcccgga acgctactca acagccggtc aacccggagc ggagctacaa caggcggccc
     3481 ctggaacagc gctcaacacg gcaatctggc accctgacaa tcctcacgca ccactagaat
     3541 gagtatacat ctccctgctt tattctccct tacacgccac tttatgatgg agagtagcta
     3601 cagatgtgat gagagcgtct catgaaaccc ccttcgaggc tgtattgtaa ggatgggcaa
     3661 gaaactttag agaagactgt cgataaatat tacataagac aaattcacac tctactcttg
     3721 acttctcgag ccgattaatg gtatcaatat atagaccgac tggtcagtct attagatttg
     3781 tcaaggtgca aatggcctgt aagggcgcaa tgaattgcgc cctggtggta gcgcttgatc
     3841 ggggacgagg gcgcaatgaa ttgcgcccct acaggccatt tgcaccggtg tgaagcgaaa
     3901 agtgtgaatg tatgtcgcga cgcccggatg taagtggagt gcgaaaaaat cagatattgg
     3961 acgcggcgat ggtcgtgttt gccaggatgg gatttcacga agctcgcatg gatgacatcg
     4021 tggcggaatc tggtctgagc aagggcgcgc tgtactggta tttcaaaagt aaagaggaca
     4081 ttatta
//
LOCUS       DASZAK010000847         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_553716, whole genome shotgun sequence.
ACCESSION   DASZAK010000847 DASZAK010000000
VERSION     DASZAK010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553716"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2791
                     /locus_tag="VGT44_14960"
     CDS             <1..>2791
                     /locus_tag="VGT44_14960"
                     /inference="COORDINATES: protein motif:HMM:NF015501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tc toxin subunit A"
                     /protein_id="HEV2582154.1"
                     /translation="AYHLEVSGQVDEVTASKLNERLLALAGGEEHEEHRQEHEEHEEH
                     EEHREEHEERGDEENGDEDDHKNGNGHGKGGEQRYVVKGTVRDANGDSLANLTVQVFE
                     KTLRKEQMLAFGQTMTDGTYTITYRPPSTPQAAASPSFNIVVKVLNQQGEVWLVSPVI
                     FGAKPLELVDLMDGGVYTGLSAFETVMAAIQPSLDGLPVAQLVETDDHPDITFVASDS
                     KQDPEHIMQLVVSHLLSQTTDLPPELFYAFLRQNLPTTVPKSLLAASDKFKKIDVVLH
                     NTLAGIAALTPDVQKNALDHAFQKNLIPLRLKAEQDQILQKLQALRVSNTLEQPYLTG
                     KVSLKTMLSLSVLPDTLYTQFAQLYLSSGGTSHNFWLALAKQSGFTPDVVADLRLTLG
                     VGSMVKNYPSAVTFLKQGFQQQTYTTLQDLAKLDINAWETIVKQTGYPSNITGATEND
                     KISVFAHEIFARVEQAFPTTSFVAQVARSQVSLVQYQPQVVQFFNDNPTLNLKRIHVD
                     RYLKDNGEQALRNIPVELRAPVIQQAKAMQRVLRLAPTTNAATALLSMKIHTAQQIYF
                     TAQSTFVTNAVAQGATKTAAQRVYQRAGQTYAMTLARIAELNAQFNGPAFSTIPSSSP
                     NSQTAQQLKDYPDLQTLFGSLDYCTCDECNSVLGAAAYLVDLLLFLKQRTTAGVTAKD
                     VFLKRRPDVGTLLLNCENTNTALPYIDLVCELLEEEIAPTGQGRQTTWTEDELRANPQ
                     YVNAPVYNTNLVQAHYPNTLPFNLWLEEARVYLNHLGVSRTQLMKTFQNRAASPSVPS
                     DSDIAAEYFGISPEEEALICNADAANQPTYWGETDPVTTLKIVATFLQQSGLSYNQLL
                     ALLAVGFIQDSPATSVLQSTSNACDTQQQTITNLSLSRLDKMHRFIRMWRKTSWQMWE
                     LDRLILSPKIGNGTLD"
BASE COUNT          672 a          831 c          732 g          556 t
ORIGIN      
        1 gcctatcacc tggaagtaag cgggcaggtg gatgaggtca ctgcatcgaa gctgaacgag
       61 cgactactgg ctcttgccgg tggtgaagaa cacgaagaac accggcaaga gcatgaggag
      121 catgaggagc atgaagaaca tcgagaggag catgaggaga ggggagatga agagaatggc
      181 gatgaggacg accacaagaa cggcaatggg catggtaaag gtggtgagca gcgatatgtg
      241 gtgaagggga ccgtgcgtga tgccaacggc gatagccttg ccaatctcac cgtgcaggtc
      301 ttcgagaaaa cgctgcgcaa ggaacaaatg ctagctttcg gccaaacgat gacggatggt
      361 acatacacga tcacctatcg tcctccttcc actccccagg cagcggcgag tccatcattc
      421 aatattgtcg tgaaggtact gaaccagcag ggcgaggtct ggttggtttc gccggtaatt
      481 tttggcgcga agcccctgga actggtggat ttgatggacg gtggcgtcta tactggcctc
      541 tcggcgtttg aaaccgtcat ggccgctatt caaccttcgc tggatggcct acccgtagca
      601 caactggtcg agactgacga tcacccggac atcaccttcg ttgccagtga tagcaagcag
      661 gaccctgaac atatcatgca gcttgtcgtc tcgcacctcc tatctcagac aaccgatctc
      721 ccgccagagc tcttttacgc ctttttgcgg caaaatctgc ccacaacagt acccaaatcg
      781 ctgctggcag cctcggataa gttcaagaag atcgatgtcg tgctgcataa cacgctggct
      841 ggcatcgctg cactcactcc cgatgtgcaa aagaacgctt tagatcatgc ctttcaaaag
      901 aacctgatcc cgctcaggct caaagcagag caagatcaga tactccagaa gctccaggcg
      961 ctgcgcgtaa gcaacacact cgaacagccc tatctgaccg gcaaggtcag tttaaagaca
     1021 atgctctcgc tatccgtatt gccggacacc ctctacaccc agttcgcgca gctctatctg
     1081 agcagtggag gaacatcgca caatttctgg ctggcgctcg ctaagcagtc aggcttcacg
     1141 ccggacgtgg tagcagatct gcggctgacc ctcggcgtag gatcgatggt caagaattac
     1201 ccgtccgccg tcacattcct caaacaggga tttcagcagc agacgtatac taccttgcaa
     1261 gacctggcga aattggacat caacgcctgg gaaacaatcg tcaagcaaac gggctatccg
     1321 agcaatatta cgggcgcgac cgagaacgac aaaattagcg tcttcgctca tgagattttc
     1381 gctcgcgtgg aacaagcctt ccctacgacc tcgttcgtgg cccaggtggc gcgcagccag
     1441 gtttcgctcg tgcagtacca gccgcaggtt gtgcagtttt tcaacgataa ccccacgctc
     1501 aacctgaagc gcatccatgt tgatcgttac ctgaaagata atggcgagca ggccctgcgc
     1561 aacattcctg tagaattgcg tgcgccagtc attcaacagg cgaaggccat gcagcgcgtt
     1621 ctccggcttg ctcccacgac gaacgccgcg accgctcttc tgtcgatgaa gattcatacc
     1681 gcgcagcaaa tctacttcac tgctcaatcc acctttgtca cgaatgccgt ggcacaggga
     1741 gcgacgaaga cggcagcgca gcgcgtctac cagcgtgcag ggcagacgta tgccatgacg
     1801 ctggcgcgta tcgcggaact caacgcccag ttcaatggcc ccgccttcag cacaattcct
     1861 tcaagctcgc ccaactccca aacggcacag caactcaagg actaccctga cctgcaaacg
     1921 cttttcggct cgctcgatta ctgcacctgc gacgagtgca actccgtgct aggcgcggcg
     1981 gcctacctgg ttgatctgct cctcttcttg aagcagcgaa ctaccgcggg cgttacggcc
     2041 aaagatgtct tcctcaagcg gcgacccgac gtcggcacac tccttctcaa ctgcgagaac
     2101 acgaataccg cgctgcccta catcgacctg gtgtgcgagc tgctggaaga ggagattgct
     2161 cccaccggcc agggacgcca gaccacctgg actgaggacg aattgcgtgc caatccgcaa
     2221 tacgtgaacg cgcccgttta caatacaaac ctcgtccagg cacattaccc gaacacgctt
     2281 cccttcaacc tgtggttgga agaggcacgc gtctacctca atcacctcgg cgtgtcgcgc
     2341 acccagctca tgaagacctt tcagaatcgc gccgcttcgc catcggtccc atcggacagc
     2401 gatatagcgg ctgagtactt cggcatctcg cccgaggaag aggcgttgat ttgcaacgct
     2461 gacgccgcga accagcccac ttactggggc gagaccgatc cggtcactac tttgaagatt
     2521 gtggcaacct tcctgcaaca gtcgggattg agttacaatc aactgcttgc gctactggcc
     2581 gttggcttta tccaggatag ccccgctacc agcgtattac agagtacctc taacgcatgc
     2641 gatacacagc agcagacgat taccaacctg agcctgtcgc gcctggacaa aatgcaccgc
     2701 ttcatccgca tgtggcgcaa aacgtcgtgg cagatgtggg agctagatcg cctcattctg
     2761 agccctaaga ttggcaacgg caccctggac a
//
LOCUS       DASZAK010000848         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_378035, whole genome shotgun sequence.
ACCESSION   DASZAK010000848 DASZAK010000000
VERSION     DASZAK010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378035"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VGT44_14965"
     CDS             <1..1320
                     /locus_tag="VGT44_14965"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV2582155.1"
                     /translation="LELLNAKAPNRTLDNVLAEQQAENAGTSSRSLQQTLRIIGPGFL
                     SGMAGNDSSAVGAYSIDGASNGYGHLWLMLLSTPLYQAVQFACAKIGRVTQKGLAELL
                     REHYGRWVAVIASLILIIANVALVAADLVAIGSGFELIAGLSYVWFIVPVAGLLWYLT
                     VYRNFETIKKIFIVLSLAFVAYIITAIFSGANWGEVLLRTLVPQIDFKFASISSAVAI
                     LGATISPYTIFWQVQGEKEQKRPGPIQHKIRLAALDIGVGVISGNLVAYFIIVCTAAT
                     IFIHHGQISTAADAARALQPLLGPFAKYLFAVGLIGAGVIAIPILLASTSYAVSGTFG
                     WPAGLSKKPWQNEGFYLILTVALVVSMALALLRIDPIQLLFWANVLNGVLSPVLVVFV
                     LFVGNNRRIMSRQRLGILTNVGLIAAFIVLASATILLFYGLLTGHAS"
     gene            complement(1418..2092)
                     /locus_tag="VGT44_14970"
     CDS             complement(1418..2092)
                     /locus_tag="VGT44_14970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV2582156.1"
                     /translation="MQDPFLLRREIAAELFIVRHADAIPDADEIIPSGIYDDLPLSRR
                     GREQAQALAERLGETPFDAIYSSPLLRCRQTAAPLAERLGLTPTLIDALREVRLGNID
                     ALPQDTNDLAALTEALQKRQTDIVRLAGEAGNWDMIPDSEPSKAFRRRVVEACDGIAQ
                     QHIGQRVLIFAHGGVVNAYAAETLGLEREFFFPAANTAITVMRVAGKQRVLFTLNDVG
                     HLIKRV"
     gene            complement(2104..3144)
                     /locus_tag="VGT44_14975"
     CDS             complement(2104..3144)
                     /locus_tag="VGT44_14975"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV2582157.1"
                     /translation="MTTLPPLIDEERLRQYLARRMPGEDKPLQVERVLGGHSNETFYV
                     TRGPDETWVLRRPPRGPLLPTAHDVLREYRVLKALNTTSVPTPRVLLACDDVSVTGAP
                     FYLMERTFGMVIRTELPSYGSDAAGRAIISKALIDSLVALHGVDWQAVGLGDFGKPEG
                     YIERQLRRWTGQLDKSRQRPLPDLDAVTEWLVGHLPVSGPATIVHGDFRLDNVMFDFD
                     RPDVVAIFDWEMATIGDPLADLGYLLSHWHEPSDPEPGFASQTWTVMMQPGFLSRAEI
                     AEYYARRTGRSVRDMAFYTALAIWKLAILLEGSYSRHRAGTTDDPFFEQLEAGVPALA
                     REALHICQTANA"
BASE COUNT          613 a          994 c          970 g          728 t
ORIGIN      
        1 ctggagttgc tcaatgccaa agctcctaac cgtaccctcg acaatgtgct tgccgagcag
       61 caggcagaaa acgctggcac ttcttcacgt tcgctgcagc aaaccttacg cataatcggc
      121 cccggttttc tcagcggcat ggccggcaac gactcctctg ccgttggcgc atattctatc
      181 gatggagcga gcaatggcta tggtcatctc tggctcatgc tgctctctac ccccctgtac
      241 caggctgtcc agtttgcttg cgccaaaatc gggcgtgtaa cgcagaaagg gctggcggaa
      301 cttctacgcg agcattacgg tcgctgggta gcagtgattg cttcgcttat cctcatcatt
      361 gccaatgtcg cgctggtcgc cgccgacctg gtggctatcg gcagtgggtt tgagttgatt
      421 gccgggctat cttacgtctg gttcatcgtt cccgtggccg ggctgctctg gtatcttacc
      481 gtttatcgca attttgagac tatcaaaaag atctttattg tcttgagcct ggctttcgtc
      541 gcctacatta tcaccgcgat tttttccggc gccaactggg gagaagtctt gctgcgcacc
      601 ctcgtgccgc agatcgattt caaatttgcc agcatcagca gtgcagtcgc cattttaggc
      661 gcgaccattt caccctatac cattttctgg caggtgcagg gagagaaaga gcaaaagcga
      721 ccggggccaa tacagcacaa gattcgcctg gcggctctgg atattggtgt cggtgtgatc
      781 agcggcaacc tggtcgccta cttcatcatc gtttgcacgg ctgcgactat tttcatacat
      841 catggtcaga tcagcacagc cgccgacgct gccagagcct tgcagccgct gctgggaccc
      901 ttcgcgaaat accttttcgc ggttggactg attggggcgg gggtgatcgc tattcccatt
      961 ctgcttgcca gcacatcgta tgccgtctca ggcacattcg gctggccggc cggtctctcg
     1021 aaaaagccct ggcaaaacga gggcttttac ctgatcctga ccgttgcgct cgtcgtgagc
     1081 atggcgctgg cgctcctgcg tatcgatccc atccagttat tgttctgggc aaacgtgttg
     1141 aatggcgtcc tgtcgccggt gctagtggtc tttgtgttgt ttgtcggcaa caatcgccgc
     1201 attatgtcga gacagcgcct gggtatcctc acaaatgttg ggctgatcgc cgctttcatc
     1261 gtcctcgcct cggcaaccat actcctgttc tatggccttt tgacgggaca tgcctcataa
     1321 tacctatcga tctgtcatgg agtcagtagg ggcgagcctt tatggtcgcc cctactgact
     1381 ccatgaccct gttcggtggt caaaagtctt cgggacgcta cacgcgcttg atgaggtgtc
     1441 ctacgtcgtt cagcgtgaac agcacgcgct gcttcccggc gacgcgcatc acggtaatag
     1501 ctgtattcgc ggcgggaaag aaaaactcgc gttccagccc gagcgtctcg gcggcgtagg
     1561 cgttcaccac accgccgtgc gcgaagatca gcactcgctg accgatatgt tgttgagcga
     1621 tgccgtcgca tgcctcgacg actcggcggc ggaacgcttt cgatggctcg ctgtcaggga
     1681 tcatgtccca gttgcctgcc tcgccagcca gccgcacgat gtctgtttgg cgcttttgca
     1741 atgcctccgt cagcgcggcc aggtcgttgg tatcctgcgg tagagcatca atattgccca
     1801 gccgcacctc acgtaatgca tctatgagcg ttggagtcaa ccccagtcgt tccgccagtg
     1861 gcgcggcagt ctgacggcaa cgcagcagag ggctgctgta gatggcgtcg aatggcgtct
     1921 cgccaagtcg ctccgcgagc gcctgcgcct gctcgcgccc gcggcggctg agcggcaggt
     1981 cgtcgtagat gccgctcgga atgatttcgt ccgcgtcggg gatggcgtca gcgtggcgca
     2041 cgatgaagag ttcagccgcg atttcgcggc gcagcagaaa agggtcttgc atatggttcc
     2101 tatctatgcg ttggctgtct ggcagatatg cagcgcttcg cgagccagcg cgggtactcc
     2161 agcctccagt tgttcaaaga atgggtcgtc cgttgtgcct gcgcgatggc gcgaatagct
     2221 gccttcgagc aggatcgcca gtttccagat ggccagcgct gtgtagaagg ccatgtcacg
     2281 cactgaacgc cccgtgcgtc tggcataata ctcggcgatt tccgcgcgcg acaggaagcc
     2341 cggctgcatc atgaccgtcc atgtctgtga tgcgaacccc ggctccgggt cgctcggctc
     2401 gtgccaatgc gagagcagat agcccaggtc ggccaacggg tcgccgatag ttgccatctc
     2461 ccagtcgaaa atggcgacca cgtctggccg gtcaaagtca aacatgacat tgtccaggcg
     2521 aaaatcgcca tgcacgatgg tggccgggcc ggaaacgggc aggtgtccaa cgagccattc
     2581 ggtcacggcg tccaggtcgg gcagcggtcg ctggcgcgac ttgtcgagct gtcccgtcca
     2641 gcgccgcaac tgccgttcga tatagccctc cggcttgccg aaatccccca gcccgacggc
     2701 ctgccagtcc acgccgtgca gcgcaaccag cgagtcgatc agggctttgc tgatgatggc
     2761 gcgcccggct gcatcgctgc cgtaggatgg cagttccgtg cggatcacca tgccaaaggt
     2821 gcgctccatc agatagaagg gcgcgcccgt cacgctgaca tcgtcgcagg ccagcagcac
     2881 gcgcggcgtc ggtacgctgg tcgtgttcag cgccttgagg acgcggtact cgcgcagcac
     2941 atcgtgcgcc gtcggcaaga gcggcccgcg cggtggccgg cgcagcaccc acgtctcgtc
     3001 ggggccgcgt gtgacgtaga atgtctcgtt ggaatgcccg cccagcacgc gctcaacctg
     3061 caagggttta tcttcgccag gcatacgccg cgcgagatac tggcgcagcc gttcctcgtc
     3121 gatcaagggt ggcagggtgg tcatagcaac tcctctccag ggcgacctca acgcagcggt
     3181 cactccatag tgcaacgaca cagcagcaga gctatcagca gtgtatcaca tgtatgcata
     3241 gcgacggcaa gcgtaggtgt gtgacgaact tcacacaaaa ttgggaacct gcctgaaatc
     3301 actaa
//
LOCUS       DASZAK010000849         6892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_449397, whole genome shotgun sequence.
ACCESSION   DASZAK010000849 DASZAK010000000
VERSION     DASZAK010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449397"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..2061)
                     /locus_tag="VGT44_14980"
     CDS             complement(214..2061)
                     /locus_tag="VGT44_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEV2582158.1"
                     /translation="MQHTITDDLEALLVTLPPVIHDAVNRLENRSDLLEIVMDLGRLP
                     EGRFPEGEVILSTQPVTDADLEYVVERIGEFGDDNRAGIERTLHRISALRNRKGKVIG
                     LTCRIGRAVLGSIALIRDIVEQGQSILILGRPGVGKTTLLREIARVLADEANKRVVVV
                     DTSNEIAGDGDIPHPGIGRARRMQVARTAEQHAVMIEAVENHMPQVIVIDEIGTELEA
                     AAARTIAERGVQLVATAHGNSLGNLLVNPTLSDLVGGIQTVTLGDEEARRRHTQKSIL
                     ERKAPPTFDVVVEQQSWQELVVHRDVADTVDSMLRGQTVVAEERTRDEETGRVTARRI
                     TSGGMDVPTWGIEGFGAGSRPVSQIDRPDSNWGPVRRMGQQGPRGGSERIHGRSQAQA
                     HVQVRELAPTGTAPADVKRHNTAGLVAIDRPLLDEELYQPEKAKEQLPIHKTLRIYPF
                     GVSRDRLAESARQLRVPVIVTNNQGDADAIITLKNYYRRQPERLQHAEEEQKPIFILK
                     NNTVAQMLHALARIFDIPIDVSIDENQEEAVGGNGDGQDDPTMRALLETEDAIHQVLN
                     KGLTTAELAPANAYIRRLQHQMATRYNLSSRSRGKEPYRRVKIFRARDS"
     gene            complement(2073..3113)
                     /locus_tag="VGT44_14985"
     CDS             complement(2073..3113)
                     /locus_tag="VGT44_14985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582159.1"
                     /translation="MIRPVLYVDLPGIIYAIDRRARNKQHEFANFVCWLEPVESRIPL
                     SPILRHIFPVNTASRTWICEDHWHILGFAQVRERPSRLTWDLAYLASMVNRDVSGDEV
                     LTALLDYMLEVASSHGILRIFAKVEDEIPEMELFHKAGFQRYARQLTYVFNPESVQGD
                     FNPPSLRRWNRHHVWGLHQLYRAVTPQRVQMAEMLDNSEEFAKLYVGSLRPLPSALSR
                     GDESFICDVGVRLGAWLRLCPGHGTAPHQISLIVHPEHAELAEPIIRFGTQRLLERSA
                     RPIYCQVREYDSSVINALRSSGFEHVSTRALLVRHIALLAMNPRAVPALEQRVAYGVK
                     GLGTVNSRQTTR"
     gene            complement(3167..3778)
                     /gene="pdxT"
                     /locus_tag="VGT44_14990"
     CDS             complement(3167..3778)
                     /gene="pdxT"
                     /locus_tag="VGT44_14990"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV2582160.1"
                     /translation="MGTSSPKKLHIGVLALQGDFEAHLKMLAELGVEGKAVRLPKHLR
                     DLDGIIIPGGESTTIGKLMVLYGLHEPLQQKIREGFPIWGTCAGLILLARETDNALVD
                     QPLLASMHIRVRRNAFGSQRESFETDLSVPVLGEAPFHAFFIRGPAVESVGPEVEVLA
                     TLDDGTIVAVREGALLGTAFHPEIGGDPRFHQYFLRIAQSVKG"
     gene            complement(3782..4669)
                     /gene="pdxS"
                     /locus_tag="VGT44_14995"
     CDS             complement(3782..4669)
                     /gene="pdxS"
                     /locus_tag="VGT44_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV2582161.1"
                     /translation="MQKQIGTIQVKRSFPEMLKGGVICDVVNAAQARIAEEAGAAAVM
                     ALERVPADIRAQGGVARMTDPELIIKIKEAVTIPVMAKCRIGHFVEAEILQAIGVDCI
                     DESEVLTPADEHFHVNKHLFTVPFVCGARDLGEALRRIGEGAAMIRTKGEAGSGNIVE
                     AVRHIRAVMDGIRRLQMLPEEELMTEAKNLGAPYELVREVAQKGKLPVVNFSAGGCAT
                     PADAALMMRLGAEGVFVGSGIFKSGDPLKRAKAIVRATTHFQDPDIIAEVSRNLGEPM
                     VGINLDQLPEHEQMARRGW"
     gene            complement(4801..5235)
                     /locus_tag="VGT44_15000"
     CDS             complement(4801..5235)
                     /locus_tag="VGT44_15000"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEV2582162.1"
                     /translation="MSSEAKAPSTPSPQEDETSHLLLPPPASPGPRRGEIWDVNWSPG
                     RGAEQKGTRPALIIQNDRGNTSSSYPLTIVASMSRTERELPLHVRIAPSAENGLTDFT
                     DVKCEQVMTIEKSRLIRRRGSISSDELAKVDVALRLSLNLLT"
     gene            complement(5261..5590)
                     /locus_tag="VGT44_15005"
     CDS             complement(5261..5590)
                     /locus_tag="VGT44_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582163.1"
                     /translation="MAVVQPDFYRHENLVRKSIQIPSMQHKELKALAVELESSLGDVV
                     ETIMDLMRQDNYEQLKAAIIQKRERDLFSLSHSLDEAYAYLGSVPETLEDARFFFNAG
                     QEGMIVA"
     gene            complement(5744..6823)
                     /locus_tag="VGT44_15010"
     CDS             complement(5744..6823)
                     /locus_tag="VGT44_15010"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2582164.1"
                     /translation="MAAPIHDQPPRGISRRSVLGSLLGIATVVAVGGTALFTYGHLHP
                     TTNPITLDYPPHSNPTQQPTPTLTNKPLIHQGPDKEYTVSWSPDGTKIASAGNGEIIE
                     VWDPITGTSLFNLASGAGTVFSVAWSPDGTKIASGQKDGTVKIWDAKNGNVISTLSGH
                     SAQVNSIAWSSDSKNIVSGSGDHTARVWDVAGGNCVTVYHGHTSWLNGVAWSHNSPYV
                     VSGSGDKTAQVWDAFSGNAVYTYTRHTNEVLALAWSPDNSHVASASDDNTVQIWDTMN
                     GHPYVTYTGHTGFVVAVAWSPDGQYIASGGVDTTVQVWKAIDGTPISKFTGHKAEVEG
                     ISWSSDSKRVASASDDKTVQLWQAT"
BASE COUNT         1528 a         1866 c         1839 g         1659 t
ORIGIN      
        1 gtagctgttg cgtgtttgct caagattctc agccatacgt gctttctcct ctggtgtctc
       61 caactcggat gagtgtagcg cgcacgcctg cgaacaaaca agtggtgttt tttgatcagt
      121 catcatccgg aggtggtatt acagggcgac cgcaaggggc ctgtggtgga atcaatgccc
      181 cttgcggtcg ccttgtgccc tactggcgag tctctacgaa tcccgcgcgc ggaaaatctt
      241 cacccttcga tagggctctt tgccccgact gcgcgagctt aagttatagc gcgtagccat
      301 ctggtgctgt aacctgcgaa tataggcatt agcaggagct aattcagccg tggtcagtcc
      361 tttgttcaga acctggtgga tagcgtcttc ggtctccagc agcgctcgca tagtaggatc
      421 atcctgacca tcaccattcc cccccacagc ttcttcctgg ttctcatcga tagaaacgtc
      481 tataggtatg tcgaagatgc gagccagggc atgcagcatc tgggcaaccg tattgttctt
      541 caggatgaag atcggctttt gttcctcttc cgcatgctgt aggcgttccg gctggcgacg
      601 atagtagttt ttcagcgtga taatcgcgtc ggcgtcacct tgattgtttg tgacgattac
      661 cggtacgcgc aactggcgtg cagattcagc caggcgatcg cgactcaccc caaacgggta
      721 aatgcgcagc gtcttgtgga tgggcaattg ctccttcgcc ttttcaggtt gatacagctc
      781 ttcatcgagc aaagggcgat caattgctac cagccctgcc gtattatgtc gcttgacatc
      841 tgcaggggcg gttcctgtcg gcgccaactc ccttacctgt acgtgagcct gagcttgact
      901 gcgaccgtgt atgcgttcgc taccgccgcg aggcccctgt tgtcccatgc gacgtacagg
      961 tccccagtta ctgtcaggac ggtcgatctg gctgaccggg cgtgatcctg cgccaaagcc
     1021 ttctataccc caggttggca catccatacc accagaggtg atacggcgcg cggtgacgcg
     1081 cccggtctcc tcatcgcgcg tgcgctcctc agcaacgaca gtctgcccgc gcaacatgct
     1141 gtccaccgta tcagcgacat cacgatgcac gaccagctcc tgccagctct gctgttcgac
     1201 caccacatcg aaggttggcg gtgctttacg ttccagaatg ctcttctgtg tatgacgtct
     1261 gcgagcctct tcatctccaa gtgtgacggt ttggatacca ccaacgaggt cagaaagcgt
     1321 cgggttcacc agcaggttac caagcgagtt gccgtgcgca gttgcgacaa gctgtacacc
     1381 gcgttccgca atggtgcgag ccgcggcggc ttccagctca gtaccgattt catcgattac
     1441 aatgacctgt ggcatgtggt tctccacagc ctcgatcata acggcgtgtt gttcggcggt
     1501 gcgagccact tgcatacggc gcgctcgacc aataccggga tgaggaatat ctccgtctcc
     1561 cgcgatttcg ttcgaggtgt cgacaacaac gacacgttta ttggcttcgt ccgcgaggac
     1621 gcgagctatt tcacgcaaca gggtggtttt tcccacacct gggcgaccaa gaatcaaaat
     1681 tgattgtccc tgttcgacaa tatcgcggat caacgcaata ctgccaagta ccgctcgacc
     1741 gatgcggcag gtcaggccga taacttttcc ttttcgatta cgcagcgcgc tgatacggtg
     1801 gagcgttcgc tcgataccag cacggttatc gtcgccaaat tcaccgatac gctcgacgac
     1861 atactcgagg tcggcgtcgg tcactggctg agtacttagg atcacctccc cttctgggaa
     1921 ccggccctca ggcaaacgcc caaggtccat tacgatctcc agcagatcac ttcggttttc
     1981 gagccgattg accgcgtcgt ggatgacagg tggcaaagtt accaacagag cttccagatc
     2041 gtcagtgatc gtgtgttgca taggcagttc tatcacctcg ttgtctggcg cgaattgacc
     2101 gtccccaggc ctttcacgcc ataggctact cgttgttcca atgcggggac agcgcgcggg
     2161 ttcattgcca acaaagctat gtgccgcacg agcaatgccc tggtcgagac atgctcaaaa
     2221 ccactggaac gcagagcgtt aataacggaa gaatcgtact cccgcacctg gcagtagatg
     2281 ggtcgtgcgc tcctttccaa aagtcgctga gtgccgaacc gtatgatcgg ttcagccaat
     2341 tcagcatgtt caggatgaac aataagcgag atctggtgtg gcgctgtgcc gtgaccgggg
     2401 cataggcgca gccaggctcc taaacgaacg ccaacgtcgc atatgaagct ttcatctccc
     2461 ctggagagag cagatggcaa cggacgcagc gaaccgacgt atagcttagc aaattcttca
     2521 ctgttatcca gcatctccgc catctgcacc cgctgtggag tgacagcgcg gtaaagctgg
     2581 tgcaatcccc atacatgatg gcgattccag cgtcgaagtg aaggtgggtt gaagtcgccc
     2641 tgtactgatt cagggttaaa gacataggtc agttggcggg catagcgctg aaatccagcc
     2701 ttatggaata gttccatttc tggaatttca tcttcaacct ttgcaaaaat acggaggatg
     2761 ccatggcttg atgcaacctc cagcatatag tcgagcagcg cagtaagcac ctcgtcgccg
     2821 gagacatcgc ggttcaccat agaggcaaga taagctaagt cccatgtaag acgcgaaggt
     2881 cgttctcgca cctgcgcaaa tcccaggata tgccagtgat cctcgcatat ccaggtgcgt
     2941 gaagcagtat tgacggggaa gatatgcctc aggataggtg acagaggaat acgagattct
     3001 accggttcga gccaacacac gaagttcgcg aattcgtgct gcttgtttcg agcacggcga
     3061 tcaattgcgt agatgattcc cggcagatcg acatatagta ccggtcgtat catccagtgc
     3121 tcccgcgtca tagggggtgg aaaactatgc cctgaccttc gcgcccctac cccttgacgc
     3181 tctgtgctat gcggagaaag tattgatgga aacgtgggtc cccaccaatc tccggatgaa
     3241 atgctgtgcc tagtaaggct ccctctcgga ctgcgacgat ggttccatcg tcgagcgttg
     3301 ccagcacctc cacctcggga ccaaccgatt caactgcagg tccgcggatg aagaatgcgt
     3361 ggaatggcgc ttcccctagt actggcacag agagatcagt ttcaaaactt tcccgctggc
     3421 tgccaaatgc atttcggcga acacggatgt gcatcgaagc cagcaagggc tggtctacaa
     3481 gcgcgttatc cgtctcgcga gccaataaga ttaatcctgc gcatgttccc caaatgggga
     3541 acccttcccg aatcttctgt tgcagtggct cgtgtaagcc atacaacacc atcaatttgc
     3601 caatggtggt actctcacca cctggtatta ttataccatc taaatcgcgc aaatgcttgg
     3661 ggagtcggac ggcttttcct tcgactccca attctgccag catcttgaga tgtgcttcaa
     3721 agtcaccctg taatgctaag acgccgatgt gcaacttttt tgggctagat gttcccatgc
     3781 tctaccaccc gcgtcgcgcc atctgctcat gttcaggtaa ctgatccagg ttgatgccga
     3841 ccatcggctc acccaggttt cgcgagacct cggcgatgat gtcggggtcc tggaagtgcg
     3901 tggtagcgcg cacaatcgct ttggcgcgtt tcagcggatc gccggacttg aagatgccag
     3961 agccaacaaa gacaccctct gcgcccaggc gcatcatcag ggcagcatcg gcgggagttg
     4021 cgcagccgcc tgccgaaaag ttcacaacgg gtaacttacc tttttgagca acctcgcgca
     4081 ccagttcata gggcgcaccg aggttttttg cttcagtcat caattcttct tcgggcagca
     4141 tttgcaggcg gcgaatgcca tccataacgg cgcgtatatg gcgcaccgcc tctacgatgt
     4201 tgccggaacc tgcctcgcct ttcgtgcgta tcatcgcagc accttcgcca atgcgtcgca
     4261 gcgcctcgcc aagatcacga gcgccacaca caaatgggac ggtgaagaga tgtttattga
     4321 catggaagtg ttcgtcggct ggcgttaaca cttctgactc gtcaatgcag tccacaccaa
     4381 tggcctggag aatttcggct tcgacaaaat gcccaatacg gcacttcgcc atgacaggaa
     4441 tagtgacggc ctctttgatc ttgatgatga gttcagggtc ggtcatgcgc gcaacgccgc
     4501 cctgagcgcg aatatcggcc ggcacgcgct ccaatgccat tactgctgct gctccagctt
     4561 cttctgcgat gcgtgcctgt gcggcattta cgacatcaca gatcactcct cctttgagca
     4621 tttctggaaa actgcgttta acttgtatcg ttcctatctg tttttgcatg agtatatctc
     4681 tcctggttga cccgtgggaa gtgtaacgtt tgtactacat tttatcatac ggggaaggcg
     4741 tgctgctaca gccaagtgga gagggggatg ccagccagga ttatggaggg gggaggaata
     4801 ttatgtcaac aagttgaggc ttaagcgcaa ggcgacatcg actttagcca attcgtcgct
     4861 acttatggag ccgcgacggc gaatcaggcg ggatttttca atggtcatta cctgttcgca
     4921 tttgacatcg gtgaagtcgg tcaggccgtt ttctgctgag ggggcgatac gcacgtgcag
     4981 tggcagttcg cgttcggtgc gactcatcga ggcgacgata gtgagagggt aagaggaaga
     5041 ggtattcccc cggtcgttct ggataatcaa agcgggccgc gtaccttttt gttcggcgcc
     5101 tcgaccagga gaccagttca cgtcccaaat ctcgccgcgc ctgggaccgg gggaagcggg
     5161 cggcgggagt agcaggtgac ttgtttcatc ttcttggggg ctgggcgtag atggtgcctt
     5221 agcctcacta ctcatgcaac taccttacaa atcttacact ttacgcaaca atcatacctt
     5281 cttgaccggc attaaaaaag aagcgtgcgt cttcaagagt ttccgggacg gaaccgagat
     5341 aggcgtaagc ttcatcgagg gagtggctga gggagaagag atcgcgttcg cgtttctgta
     5401 tgatcgccgc tttcaactgt tcgtaattat cctggcgcat caggtccata atcgtttcca
     5461 caacgtctcc tagcgaggat tctagttcaa cggcgagggc ttttagctct ttatgttgca
     5521 ttgaagggat ttgtatcgat ttgcggacga ggttctcatg acgataaaaa tcaggttgca
     5581 caacagccat aaaactgcct cctgttacta acgcaaagcc ttccatttca tctgctctta
     5641 ttgtaaacgg tgacttctaa aaagtcaata aatataattt tggaaaaatg gaaaaatgga
     5701 atttggccag acaggcccgg ggtacttaga taagcaagta atattacgtc gcttgccaca
     5761 gttgtactgt cttatcgtca cttgcagatg cgacacgttt gctatcggat gaccatgata
     5821 ttccctcgac ctctgcttta tggccggtaa acttagagat gggagtcccg tcgatagctt
     5881 tccaaacctg caccgttgtg tccacaccac cggaggctat gtattggcca tcgggcgacc
     5941 acgccacagc taccacgaaa ccggtatggc cagtgtaggt gacataagga tgcccattca
     6001 tcgtatccca tatctgcact gtgttgtcgt cactggcaga cgcgacgtgg gagttatcag
     6061 gcgaccaggc cagtgccagg acttcattgg tatgacgggt gtaggtgtac acagcatttc
     6121 cactaaaggc atcccatacc tgggcagttt tatctccact gcctgacact acatatggac
     6181 tattatgcga ccaggcaacg ccgtttagcc aggaggtgtg gccatgatag acagtgacac
     6241 agttcccacc ggcaacgtcc cataccctgg cggtatgatc cccgctgcct gacacgatat
     6301 ttttgctatc ggacgaccag gctattgagt tcacctgagc ggaatgacca ctgagggtgg
     6361 agataacatt cccattcttg gcatcccata ttttcaccgt tccatctttt tgccccgagg
     6421 ctattttcgt tccatcaggt gaccaggcaa cgctaaacac ggtaccggca ccgctcgcca
     6481 ggttgaagag cgaggtccct gtgataggat cccatacctc tattatttca ccgttgccag
     6541 cagaggctat tttcgttcca tcaggtgacc acgaaacagt atattcctta tctggaccct
     6601 ggtggataag aggcttgttt gtgagagttg gggtgggttg ctgagtcgga ttagaatgcg
     6661 gcgggtaatc tagagttatt ggattggtcg ttggatgtaa atgcccgtat gtgaacaatg
     6721 ccgtacctcc aactgcgacg acagtagcaa tgccgagtag actacccaga actgagcgtc
     6781 gcgagatacc acgcgggggt tgatcatgga ttggcgcggc catcaccgtc tgaggcacca
     6841 gtgcaggagg agtgcgagat ggtgtatgag gcgccactcc aggagtagca gg
//
LOCUS       DASZAK010000850         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_271069, whole genome shotgun sequence.
ACCESSION   DASZAK010000850 DASZAK010000000
VERSION     DASZAK010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271069"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1181
                     /locus_tag="VGT44_15015"
     CDS             <1..1181
                     /locus_tag="VGT44_15015"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2582165.1"
                     /translation="GESGGQTGRDEVRPYDTVGYNEVVARGAERADDYLDDLVEDLSP
                     DDTAVILYTSGTTGNPKGVELSHQNIVSNAVALSGVAVENTGDPLARSEDVALAVLPL
                     AHAYGLTMSNSAYVNGAAVVMLPRFDTTAVFSAIERYRVTGFAGVPAMFVALLYTPDG
                     EKYDTSSLRLVGSGSAPLPLAILEAFEQKFNVPINEGYGLSEATTVVTAHSEGMPRKP
                     GSVGKAVTGVEVQIFDENDQPLPPGEIGEVVARGSGIMKGYYNMPDASRDALRNGWLH
                     TGDMGRLDEDGYLYIVERKKDLIIRGGLNIYPRDVEEVLNSHPHVIESAVVGVPSERM
                     GEEVKAFVVTSGAVDAETLKAYCRERLANYKTPSFIEFVNSLPRNAIGKIDKKQLRNR
                     "
     gene            1218..2174
                     /locus_tag="VGT44_15020"
     CDS             1218..2174
                     /locus_tag="VGT44_15020"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEV2582166.1"
                     /translation="MSIDMPLEQASLQQLQQTPIDNVLRIIDEGLVHLPSYRELYYRW
                     ERQQWRAQDIDFSEDQRQWANMTHDEHMMRIYGLSAFFQGEACVTDTLAPYILAMPEE
                     EMRIFVTTQLVDEARHTIFFSRFFREVMGVEAPQLEEQLSFARQYMNENLKYILIEAL
                     TEVAERIRQEPGNMAHLVEGITLYHVVIEGTMALAGQRGILEQYRRFNIFPAFRGGFT
                     AVARDESRHVVFGVKFLRDMIQRDNANARVVQAAIEKYAPTSIEAISPAPEQVQRMLA
                     NQLDPWITPRYAVESLVKKLRVVGLSMNLPIPEIPPEPVFAQ"
     gene            complement(2209..>3523)
                     /locus_tag="VGT44_15025"
     CDS             complement(2209..>3523)
                     /locus_tag="VGT44_15025"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEV2582167.1"
                     /translation="LILVLWLAGFVLTGKNAPLVLDPATILLLLLGLHWWAIGVRKFQ
                     QPISSETRTLLFHLGGPCLALALVVGMHLSLIDQPLALFLTTIVVLWCWLRGMTRTRA
                     SRRDEHMKTVFQRAFFMMLAFVMLSALVPSLATELLAPLTLALPLFFLSGLVLFSLLR
                     LKTTGQAYQRRFTQDQRADPTRLWSVLVTVFWTSMVTLAFFVETAAFPLVLVLLAPLL
                     APLASAFRNLNAFLDALLQQKPIAPLIIHKKITLLKQLPAPPFHNPVLVVILTLITFG
                     MMGLILVIVIKIWHSNYHYTREDETRQGIALRAVRKERQQKRASSAREQLDPISVRAH
                     YREFLRLMARQGTSLERFPYETPSEYQQRLYSVLESLPPQQQERFPDAILDELTGAYM
                     QERYGGKRLDDRPYTYWHTRIRALVKRFRRRSRKRDHLSIEEIDP"
BASE COUNT          814 a         1005 c         1029 g          675 t
ORIGIN      
        1 ttggcgagag cggcgggcag acaggacgcg atgaagtgcg tccctacgac actgtggggt
       61 acaacgaggt ggtggcgcgt ggggcggagc gcgcggatga ttacctggac gacctggtgg
      121 aggacctctc gccagacgat actgccgtga tcctctatac ttctggcaca acgggcaatc
      181 cgaagggtgt agaactgtca catcagaata ttgtctcgaa tgcggtggcg ttgagcggcg
      241 tcgctgtaga aaataccggc gatcccctgg cgcgctcgga agatgtggcg ctggcggtgc
      301 tgccgctggc tcatgcctat ggcctgacca tgtccaactc ggcctacgtg aacggcgcgg
      361 cggtggtgat gctgccgcgc ttcgatacaa cggcggtctt ttccgccatc gaacgctatc
      421 gcgtgacggg cttcgcgggc gtaccggcca tgttcgtcgc gctgctctat acccctgacg
      481 gagagaagta cgataccagc agcctgcgcc tggtcggcag cggctccgcg cctctgcccc
      541 tggcaatact ggaagccttt gagcagaaat tcaacgtccc gatcaacgag ggctatggcc
      601 tctccgaggc gacgacggtg gtgactgctc actcggaggg tatgccgcgc aagcccggct
      661 cggtaggcaa ggccgtgacg ggcgtcgagg tgcagatttt cgatgagaat gaccagcctc
      721 tgccgccggg cgagatcggc gaagtagtcg cgcgcggctc tggcattatg aagggctact
      781 acaacatgcc ggatgcctcg cgcgatgccc tgcgcaacgg ctggctgcac accggcgaca
      841 tgggtcgcct ggacgaagac ggctacctgt acatcgtaga acgtaagaag gacctgatta
      901 ttcgcggcgg cctcaacatc tacccgcgcg acgtggaaga ggtcttgaac agccacccgc
      961 atgtgatcga atcggcggtc gttggcgtgc cgtccgagcg catgggcgaa gaagtcaaag
     1021 ccttcgtcgt gacgagcggc gcggtcgatg ccgagacact aaaagcgtat tgtcgtgaac
     1081 ggctggcgaa ctacaaaacg cccagcttta ttgagttcgt caactcgctt ccccggaacg
     1141 ccattggcaa aatcgacaaa aagcaattga gaaatcgttg attggccagg gcgaccgcaa
     1201 ggctaaaagg agaaaccatg tcaattgata tgcccctgga acaggcttca ttacagcagc
     1261 tacagcagac ccccatcgat aacgtgctgc gcatcatcga cgaagggctg gtgcatctgc
     1321 catcgtaccg cgaactgtac tatcgctggg agcggcagca gtggagagcg caggatatag
     1381 atttcagcga agaccagcgc cagtgggcga acatgaccca cgacgaacac atgatgcgta
     1441 tctacggcct ctccgctttt ttccagggcg aggcatgcgt gaccgatacg ctggccccct
     1501 acatcctggc catgccggaa gaggagatgc gtattttcgt cacaacccag ctcgtcgacg
     1561 aagcgcgcca taccatcttc ttctcgcgct tcttccgcga ggtgatgggc gtcgaggccc
     1621 ctcaactcga agagcaactc tcattcgcgc gccaatatat gaacgagaac ctcaagtata
     1681 ttctgatcga agccctcacg gaggtagccg agcgcatccg gcaggagccg ggcaacatgg
     1741 cgcatctcgt cgagggcatc acgctctacc acgtcgtcat cgaaggcacg atggcgctgg
     1801 ccgggcagcg cggcattctc gaacagtatc gccgcttcaa catcttcccc gccttccgcg
     1861 gcggcttcac cgccgtcgcc cgcgacgaat cgcgccacgt cgtcttcggc gtcaaattcc
     1921 tgcgcgacat gatccagcgc gacaacgcca acgccagggt cgtgcaggcc gccatcgaaa
     1981 aatacgcacc gacctccatc gaggccatct cgcccgcgcc agagcaggta cagcgcatgt
     2041 tggccaacca gctcgatccc tggatcaccc cgcgctacgc cgtcgaatcg cttgtcaaaa
     2101 agctgcgcgt cgtcggcctg agcatgaatc tacccatccc ggagatccca cccgaacccg
     2161 tctttgcgca ataatgcgat ataaaaaccg gaatagaccc ttgagtactc aagggtctat
     2221 ttcttcgatc gaaagatgat cgcgctttcg agaacgtcgc cggaaacgct tgactaacgc
     2281 acgtatgcgt gtgtgccagt acgtgtatgg gcggtcatcc aggcgcttgc ctccataacg
     2341 ctcctgcata tatgccccgg tcagttcatc caggatggca tcggggaagc gttcttgttg
     2401 ctgcgggggt aacgattcca acacgctata caagcgttgt tgatactcgc tgggcgtttc
     2461 ataaggaaag cgttcaagag atgttccctg ccgcgccata aggcggagaa actcacgata
     2521 gtgcgcgcga actgagatcg gatcaagctg ctcccgtgca gatgacgctc tcttctgctg
     2581 gcgttctttt cggacagcac gaagagcgat gccctgccga gtttcatcct cccgtgtata
     2641 gtgataattg ctatgccaga ttttgatgac gatcaccagg atgagaccca tcatcccaaa
     2701 agtaatgagc gtcaggataa caacaagcac aggattgtgg aagggaggcg ctggaagttg
     2761 tttgagcaat gtgatcttct tatgtatgat aagtggcgcg atgggctttt gttgcaatag
     2821 cgcatcaagg aaggcattca ggttcctaaa cgcgctcgca agcggcgcga gaagaggggc
     2881 aaggagaact aacacgagtg gaaatgccgc cgtttctaca aagaacgcca gggttaccat
     2941 actcgtccag aacaccgtca caagcacgga ccacagacgt gttggatcag cacgctggtc
     3001 ctgcgtaaac cgcctctggt atgcctgccc cgttgtcttg aggcgcagca gcgaaaagag
     3061 aaccagcccg ctcaagaaga acagtggtag cgcaagagta agcggagcaa gcagttctgt
     3121 ggcaagcgat ggaacgagcg ctgacagcat tacaaacgcg agcatcatga agaacgctcg
     3181 ttggaagact gttttcatgt gttcatcgcg acgactcgct cgtgttcgcg tcatgccgcg
     3241 tagccagcac cagaggacaa ctatcgtagt gaggaacaag gccagaggct gatctatgag
     3301 agacagatgc atcccaacga cgagcgccag cgccaggcac ggtcccccca gatggaacag
     3361 gagtgtgcgc gtctcagaag aaatcggttg ctggaacttt cttactccta tggcccacca
     3421 gtgaaggccg agaagtagga gcaggatggt tgccggatcg agtacaagag gcgcattctt
     3481 cccggtcaac acaaatcctg caagccacaa cacgaggata agt
//
LOCUS       DASZAK010000851         2987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_93923, whole genome shotgun sequence.
ACCESSION   DASZAK010000851 DASZAK010000000
VERSION     DASZAK010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93923"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VGT44_15030"
     CDS             complement(<1..582)
                     /locus_tag="VGT44_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEV2582168.1"
                     /translation="MFNQVLNPTGNLGLTVVAALIPVIVLLVLLAVFRVTAWLATLIG
                     SILTLIVAIAIWQLPLGAGIQSYLIGSANGIWAVDWITFWGVVIFNTLKLTGLFDVFK
                     NWLIDQATADVRVQAILLAWALGALLEGLVGFGYPWAVVAPILVALGVVELDALRVAA
                     IANNAPVSYGALGAPIIALAAVTSLPLLSLSASV"
     gene            806..1858
                     /locus_tag="VGT44_15035"
     CDS             806..1858
                     /locus_tag="VGT44_15035"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2582169.1"
                     /translation="MTIKNSVGIWAFGPNATRFVPVGYHPETINEDMVTRTKRVVDGL
                     GDIVDGLEYHYPGEIHEDTLAAIQAALGTVDIYCIAAGLHPDPTYKLGVFINPDAALR
                     RRGIDTLKRGVDLAAVVGAHFIIWPGAEGYNYNFQRDYAVTWDRFVDGVGEVVAHAAS
                     KGVTVFLEHKNSEPAMNILMRNIAATLYTIDRVRRLGVDTSRLSVNMDWQHLIMNGEN
                     LAEYAALLHSEGKLGHQHANSGWGTFDDDNMVGAAFFMQTLELAMTLQDVGYGSDGKR
                     IGYDLYPYTEDQVAAVRRSVLQWAFIDDLARKIDRAALNEARAHADAVEGYRIVYEAM
                     GLDKAYTERIISQRKK"
     gene            1898..>2987
                     /gene="xylB"
                     /locus_tag="VGT44_15040"
     CDS             1898..>2987
                     /gene="xylB"
                     /locus_tag="VGT44_15040"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HEV2582170.1"
                     /translation="MDALLGIDIGTTGVKAALFAAEDGRTLSSAFVDYPLYHPHPGWA
                     EQQPEDWWQATLTAIRQCLLDGAIAGAPAAEVRGVGLSGQMHGVVLLDETYKVLRPCI
                     IWADQRSDAQCRWMTGRVGASRLIDYVSNPALPGFSAPKALWVRDNEPEIFARAHVLL
                     LPKDYIRFRLTATLAMEISDAAGTCLLDVRHGKWSREVLEAIGFDVALLPPVVPADAV
                     AGQITPEVERLTGLPAGTPVAGGGADNACGAVGNGVVRPGLALVSIGTSGVVLAHTDQ
                     PQVDRSGPVPRIHTFNHAVPGAWYLMGVTQGAGLSLRWLRDNIGLPERALERWTGIDA
                     YEMLTKEAEGVAAGSGGLLFLPYMQGERT"
BASE COUNT          610 a          881 c          935 g          561 t
ORIGIN      
        1 cacagaggcc gacagtgata acaacggcag gctggtgacg gctgccagtg cgatgatcgg
       61 cgcgcccaac gcgccatagg agaccggagc gttgttggca atcgctgcca cacgcagcgc
      121 atccagttcg accacaccca gggcaaccag gattggggcg acgacagccc agggatagcc
      181 gaagccaacc aagccttcaa gcaaagcgcc gagggcccag gccagcagaa ttgcctggac
      241 gcgcacatcg gcggtggcct ggtcgatcag ccagtttttg aagacatcga agagtcccgt
      301 cagcttcagc gtgttgaaga tgaccacgcc ccagaaggtg atccagtcca cagcccagat
      361 gccattggcg ctgccgatga gataggactg gattcccgcc cccagtggca gttgccagat
      421 cgcgatggcc acgatcagcg tgagaatgga gccgatcagc gtggcaagcc aggctgtgac
      481 tcggaagact gctaacagta ccagcagcac aatgaccggg atgagtgcgg ctactactgt
      541 cagaccaaga ttgcccgttg gattgagtac ctgattgaac atgtaacctc ctcgcttttg
      601 atacctggaa actaaaaaat gccctcctac cgcaatttta cacaagtttt acagtatttg
      661 ccactgatat gttcgcgtat ctaaaacggc gtgtcgaaca ccatgcagcc accttttccc
      721 tatgctatac taaaggcaac tatttctgcc ggaatgagtt attgagcgca ttcgagacac
      781 atcagaagga aggggaagca cacatatgac catcaagaac agcgttggaa tctgggcgtt
      841 tggaccaaat gccacgcgct ttgtgccggt tggctatcat cccgagacga tcaacgagga
      901 tatggtgacg cgcacgaagc gcgtcgtgga tggtctgggc gatatcgtcg atggtctgga
      961 ataccattat cccggcgaaa tccacgaaga tacgcttgcc gcgatccagg ccgccctggg
     1021 gacggtggat atttactgca tcgcggcggg actgcatccc gaccccacct ataagctggg
     1081 ggtattcatc aatccggatg ccgcgctgcg ccgcaggggc atcgataccc ttaaacgcgg
     1141 cgtcgatctc gcggctgttg ttggcgcaca cttcatcatc tggcccggcg cggaaggcta
     1201 caactacaat ttccagcgcg actacgctgt gacctgggat cgcttcgtcg atggcgtcgg
     1261 cgaggttgtg gcgcacgcgg catcgaaagg cgtcaccgtc ttcctggagc ataagaacag
     1321 cgaaccggcc atgaatattt tgatgcgcaa catcgccgcg acgctctaca ccatcgaccg
     1381 tgtgcgccgc ctgggggtcg atacctcacg cctctcggtc aacatggact ggcagcacct
     1441 tatcatgaac ggcgagaacc tggcggaata cgctgcgctg ctgcattcgg agggcaagct
     1501 cggccaccag cacgccaatt cgggctgggg aaccttcgac gacgataaca tggtcggcgc
     1561 ggctttcttc atgcagacac tcgaactcgc catgacgctg caagatgtcg gctacggctc
     1621 agatggcaaa cgaatcggct atgacctcta tccctacacc gaagaccagg tcgccgccgt
     1681 gcgccgttcc gtcctgcaat gggccttcat cgacgacctc gcccgcaaga tcgaccgcgc
     1741 tgccctgaac gaggcgcgcg cccacgccga cgccgtagaa ggttatcgca tcgtctacga
     1801 ggcgatgggg ctggataagg cgtataccga gcgtattatt agccagcgaa agaagtaggt
     1861 cgtcgaatca gcgttcgcac gtttaggggg aatctgcatg gatgcattgc tcggcatcga
     1921 tatcggcaca acgggcgtga aggcggctct gttcgcggcg gaggatggac gcacgctctc
     1981 atcggccttc gtcgattatc cgctctacca tccacatccg ggatgggctg agcagcagcc
     2041 ggaggactgg tggcaggcca cgctgaccgc catccggcaa tgcctgctgg atggcgcgat
     2101 tgcgggggcg ccagcggcgg aggtgcgcgg cgtcggcctc tccggtcaga tgcacggtgt
     2161 ggtgctgctt gacgaaactt ataaagtctt gcgaccatgc atcatctggg ccgaccagcg
     2221 cagcgatgcg cagtgccgct ggatgaccgg gcgcgtcggc gcgtcacgcc tgatcgacta
     2281 tgtgagtaac cccgcgctgc ccggcttttc cgcgccgaaa gcgctgtggg tgcgtgataa
     2341 cgagccggag atttttgcgc gcgcgcatgt cctgcttttg ccgaaggact atatccgttt
     2401 tcgcctgacc gcgacgctgg cgatggagat atccgatgcg gcgggcacct gtttgctcga
     2461 tgtgcggcat ggcaagtggt cgcgcgaggt gctggaggcc atcggcttcg acgtcgcgct
     2521 gctgccgccg gtcgtgccag ccgatgccgt cgcgggccag ataaccccgg aggtcgagcg
     2581 cctgacaggg ctgccagccg ggacgccggt cgcgggcggg ggagccgata acgcctgcgg
     2641 cgcggtaggc aacggcgttg tgcggccagg gctggcgctg gtttcgattg gcacaagcgg
     2701 cgttgtgctg gcgcataccg atcaacccca ggtagaccgc tccggcccgg ttccgcgcat
     2761 ccacaccttc aaccatgccg tgccgggtgc gtggtacctg atgggcgtga cgcagggcgc
     2821 gggcctctcg ctgcgctggc tgcgcgacaa catcggtctg cctgagcgcg cgttggaacg
     2881 ctggacaggc atcgatgctt acgaaatgtt gacgaaggag gctgagggcg tagcggcagg
     2941 cagcggagga ttactctttt tgccctacat gcagggcgag cgcacac
//
LOCUS       DASZAK010000852         1570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_590789, whole genome shotgun sequence.
ACCESSION   DASZAK010000852 DASZAK010000000
VERSION     DASZAK010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_590789"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..>1570)
                     /locus_tag="VGT44_15045"
     CDS             complement(44..>1570)
                     /locus_tag="VGT44_15045"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEV2582171.1"
                     /translation="GNRCHIRAFDGAPVFHLVMGPTASFAPTGYWYWRVLHRRDRERG
                     LPDIEDVYQPGAFRVPLKIGKPVTLVMSAEGALPADFGGDKHEGAITQALARHRRRVK
                     QLLDNADRTTSNLPKKDPVAARLVVAADQFIVSRPDSYAPITKLTPNRKTVIAGYPWF
                     TDWGRDSMISLPGLLLSTGRYSEARGLLKAFASYTNKGLIPNRFPDSGEVPEYNTADA
                     TLWMFHALNSYTNVTQDWSLVKELYPTLSEIIEWHRRGTLYGIGVDPADGLLRAGAPG
                     VQLTWMDAIVDGWVVTPRQGKPVEINALWYCALRYMERWANRLSYDALEYGILLSEVR
                     ENFARRYWYDAGGYLYDVVDVDGIPGQNDAALRPNQLFAASLTHRLLSEEQTRSMLQH
                     VTADLLTPMGLRTLSPHDPHYRPHFNGPRLERDSAYHQGAVWPWLIGAYIDVHLRLNN
                     DKATLLPLLKPLVQHLWNNCAGTICEVAEPEPPYTPAGCFAQAWSVGEVLRAWLAVTS
                     "
BASE COUNT          251 a          453 c          568 g          298 t
ORIGIN      
        1 gtgacaatac cgaatctggt catgaaacct catcattgcc tccctacgac gtcaccgcca
       61 accacgcccg cagcacttcc ccgacgctcc atgcctgggc gaagcagccg gcgggagtgt
      121 aggggggttc tggttcggcc acttcgcaga tggtgccggc gcagttgttc cagaggtgct
      181 gcacgagggg tttgagcagg ggcagcaggg tggctttgtc gttgttcagg cgcaggtgga
      241 catcgatgta tgcgccgatc agccagggcc agacggcgcc ttgatggtag gcgctatcgc
      301 gttcaaggcg tgggccgttg aaatgagggc ggtaatgagg gtcgtgcggg ctgagggtgc
      361 gcaggcccat gggcgtcagc aggtcggcgg tgacgtgttg cagcatactg cgtgtctgtt
      421 cctccgagag caggcggtgc gtcaaggagg cggcgaagag ctggttgggg cgcagcgcgg
      481 cgtcgttctg gccggggatg ccatcgacat ccactacgtc gtagaggtag cctccggcgt
      541 cgtaccagta gcggcgggcg aagttttcgc gcacctccga caggaggatg ccgtattcca
      601 gcgcgtcgta ggacaggcgg ttcgcccagc gctccatgta gcgcagcgcg cagtaccaca
      661 gcgcgttgat ctccaccggc ttgccctggc gcggcgtcac gacccaaccg tccacgatgg
      721 cgtccatcca ggtcaactgc acgcccggcg cgcccgcgcg cagcaggccg tcggcggggt
      781 caacgccgat gccgtagagt gtgccgcgcc ggtgccactc gatgatctcg ctgagggtgg
      841 gatagagttc cttgaccagc gaccagtcct gggtcacgtt tgtgtagcta ttcagcgcgt
      901 gaaacatcca cagcgtggcg tcggcggtgt tgtattcggg gacttcgccg ctatcgggaa
      961 agcggttggg gatcagtccc ttgttggtgt acgaggcgaa ggctttgagc aggccgcgcg
     1021 cctcgctgta tctccctgtg ctcagcagca agccgggcag ggagatcatg ctgtcgcggc
     1081 cccaatcggt gaaccagggg tagccagcga tcaccgtttt gcggttcggc gtcagtttcg
     1141 tgatgggtgc ataggagtct ggccgcgaga cgatgaactg atcggcggca acgaccaggc
     1201 gggcggcgac ggggtctttc ttcggtaggt tgctggtggt gcggtcggcg ttgtcgagca
     1261 attgtttgac gcggcggcga tggcgggcga gcgcctgggt gatcgcgcct tcgtgtttat
     1321 cgccgccaaa atcggcgggc agcgcccctt ccgcgctcat caccagcgtg accggcttgc
     1381 cgattttcag tgggacgcgg aatgcgccgg gctggtagac atcctctatg tctggcaggc
     1441 cgcgctcgcg gtcgcggcgg tgcagcacgc gccagtacca gtagccggtc ggcgcgaaac
     1501 tggccgttgg ccccatgacg aggtggaaga cgggcgcgcc atcgaaggcg cggatgtggc
     1561 agcgattgcc
//
LOCUS       DASZAK010000853         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_341293, whole genome shotgun sequence.
ACCESSION   DASZAK010000853 DASZAK010000000
VERSION     DASZAK010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341293"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..698
                     /locus_tag="VGT44_15050"
     CDS             162..698
                     /locus_tag="VGT44_15050"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV2582172.1"
                     /translation="MTWNIDPTRSHLSFAIRVMSVSTTRGRFKELRGQLHIDEQNPAN
                     SWVDAQVEVASIDTHNILRDAHLRSAAFFGVKKYPSITFTGTRVEHVGGHNYKVTGNL
                     TLHGVSRPITFDVAYPDQMMGTRASLSAKATINRRDFGLGQGVAVRFVASSMVAIEID
                     LEAIQLSVEAQKAATIAE"
     gene            complement(772..>2512)
                     /locus_tag="VGT44_15055"
     CDS             complement(772..>2512)
                     /locus_tag="VGT44_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582173.1"
                     /translation="NPNGTSNLGVSFAYWTDPIYDPTTTTPTDTTFNMLNANGQNASA
                     PWVPYTRAGCNVGAVATANMELENIATDIPTVFGANSPQAAEVKSNPNQATADFVGVS
                     VHCAQDNALCSSANNGKPDLLPDEPGGYNDFNALYGHKYVVPQINPGGPIKDLFGNVI
                     TDPSSGDIGFPGFGGITAAQSLSYVAAMQEHGVPVTYAYISDAHDNHVTGNAFGPGEA
                     GYVAQLKSYDQAFGDFFQRLENDGINKSNTLFVFTADEGDHFVGGPPSPSNCDGVTVP
                     CTYKQVGEVDGNLQGLLATEQGITTPFKVHADSAPVIYITGNPASDSSTTRALDQALG
                     KLTAQNVITGNTDQLTKYLADPVELNLLHMITADPARTPSLVMFADPNYFLFNAGASC
                     GTSCTSLDTGFAWNHGDVSPDVNTTWLGMVGPGVRNLGVTDDVWSDHTDTRVTMMVLL
                     GLQDDYQHEGRALFEALDNWAVPESLRENRDVLTNLAQAFKQINAPVGALGLATLRIS
                     TKALESSSPGDSVYTSLEEQLTDITAQRNQIAGQMLNMLEGAEFNGQPINVGQANSLI
                     DQANELLEQVDAM"
BASE COUNT          499 a          688 c          797 g          528 t
ORIGIN      
        1 aactcgccac gaagcgcacc gtcgctccct gacccaggcc aaaatcaaag caagagggtc
       61 aaggaacaca tttgtgacag acgcatgttt gtcacaaaag caccccctcg accctcttgc
      121 tttatacaga ctaccgtaca ttcattccga ggaggattac aatgacctgg aatattgatc
      181 ccacccgttc acacctctcg tttgctatcc gagtcatgtc ggtcagcacg acgcgagggc
      241 gcttcaagga gcttcgcgga caactccata tagatgagca gaatcctgcc aactcctggg
      301 tcgatgccca ggtggaggtg gccagtatcg atacccacaa tattctgcga gacgcgcacc
      361 tgcggtccgc ggccttcttt ggcgtgaaga agtatccctc tatcaccttt acgggtacgc
      421 gcgtcgagca tgtcggcggt cacaactaca aggtgaccgg gaacctgacc ttgcacggag
      481 tgtcaagacc catcaccttc gatgtcgcat accctgatca gatgatgggc acgcgcgcaa
      541 gtctgagcgc gaaggcgacg atcaatcgcc gtgattttgg cctgggtcag ggggtagcag
      601 tgcggttcgt ggcgagttcg atggtggcta tcgagattga tcttgaggct attcagcttt
      661 cagtggaagc gcaaaaggcg gcaactatag ctgaatagga tgcgaaaggc ggcggatagg
      721 tggacgcagg gaacgtccac ctatccacct cattgcttgt tggacaactt cctacatcgc
      781 atctacctgt tcgagcagct cattggcctg atcgatcagg ctattggcct gtccaacgtt
      841 gatgggctgg ccgttgaact cagcgccctc cagcatattg agcatctgtc cggcgatctg
      901 gttgcgctgg gcggtgatgt cggtaagttg ctcttccagg ctggtgtaga cactgtcgcc
      961 gggcgaactg ctctccaacg ccttcgtcga gatgcgcagg gtggccaggc cgagcgcgcc
     1021 gacgggagcg ttgatctgct tgaacgcctg ggccaggttg gtcagcacgt cgcgattctc
     1081 gcgcagcgat tccggcacag cccagttatc gagcgcctcg aagagggcac ggccttcgtg
     1141 ctggtaatcg tcctggaggc cgagcagcac catcatcgtc acgcgcgtat cggtgtgatc
     1201 cgaccagaca tcatcggtca cgccgagatt gcgcacgccg gggccgacca tgcccagcca
     1261 ggtcgtgttg acgtcgggcg agacatcgcc atgattccag gcaaagccag tgtcgagcga
     1321 cgtgcaagac gtaccacaac tggcgcccgc gttgaacagg aagtagttgg ggtcggcgaa
     1381 catgacgaga ctgggcgtcc tggccgggtc ggcagtaatc atgtgcagca gattcaactc
     1441 taccgggtcg gcaaggtatt tggtcagttg atccgtattg cccgtaatga cattctgggc
     1501 ggtcagtttc cccaacgcct gatcgagggc gcgcgtagtc gaggagtcgc tggccgggtt
     1561 gccggtgatg tagataacgg gcgcagagtc ggcatgcact ttgaagggcg ttgtgatgcc
     1621 ctgttcggtc gccagcagcc cttgcaggtt gccatcgact tcgccgacct gcttataggt
     1681 gcaggggacc gtgacgccat cgcagttgga cgggctgggt gggccgccta cgaagtgatc
     1741 gccctcgtcg gcggtgaaga cgaagagcgt gttgctctta ttgatgccgt cgttctccag
     1801 gcgctggaag aaatcgccaa atgcctgatc atacgatttt agctgagcga catagccagc
     1861 ttcgccaggg ccgaacgcat tgccggtgac gtggttatcg tgcgcgtccg agatgtaggc
     1921 ataggtgaca ggaacgccat gctcctgcat cgctgctacg taggaaagcg actgagcggc
     1981 ggtgatgccg ccaaagccgg ggaagccgat gtcgccgctg ctggggtcgg taatgacgtt
     2041 gccgaacagg tccttgatcg gcccaccggg gttgatctgc ggcacgacgt acttgtggcc
     2101 gtacaacgcg ttaaagtcgt tgtaaccgcc cggctcgtcg ggaagcaggt cgggcttgcc
     2161 attgttggcg ctggaacaga gcgcgttgtc ctgcgcgcaa tggaccgaga cgcccacgaa
     2221 gtcggcggtg gcctggttgg ggttggattt tacctccgct gcctgaggcg agttcgcgcc
     2281 aaagacggtc gggatatcgg tggcgatgtt ctcaagctcc atgttcgcgg tagcaacagc
     2341 gccaacattg cagccagcgc gcgtatacgg cacccagggg gcggaagcgt tctggccgtt
     2401 ggcattgagc atattgaacg tcgtgtcggt cggcgtcgta gtggttgggt cataaatagg
     2461 gtcggtccag taggcgaagg agacgccgag gttgctggtt ccgttgggat tg
//
LOCUS       DASZAK010000854         5318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_22129, whole genome shotgun sequence.
ACCESSION   DASZAK010000854 DASZAK010000000
VERSION     DASZAK010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22129"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(601..1767)
                     /locus_tag="VGT44_15060"
     CDS             complement(601..1767)
                     /locus_tag="VGT44_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEV2582174.1"
                     /translation="MPPYHRLGEIPHKRHTQFRKPDGTLYSEELFGTEGFSGNYSNLY
                     YTYPPTRVKKVEKFGDVSFLTEEWPQEVQRHHHLKSANLRRGGDSVSGTQVMMYNKDV
                     AIGVALPAQAMNYFYRDGDSDLVYFVHDGHGILETNFGLLPYHEGDYIVIPRGTTFRF
                     QIASDSAESRFLTIEARGTIEPPRRYLNKNGQMLEHSPYCERDIRRPEELVTHTERGE
                     FEVRIKVGNLISSYWYDFHPINTVGWDGCEYPWIFNIADFEPITGRVHQPPPVHQTFQ
                     GPNFVVCSFVPRKLDYHPLSIPVPYNHSNIDSEEMIYYVNGNFGSRRGIERSSMTLHP
                     RGIAHGPHPGAVEKSLGAERTEELAVMVDTFYPLKYTSAAQTIDDPAYPYSWIE"
     gene            complement(1779..2684)
                     /locus_tag="VGT44_15065"
     CDS             complement(1779..2684)
                     /locus_tag="VGT44_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEV2582175.1"
                     /translation="MRLVTFIAPKSHRTQAGVLRDGVVIALDYPDVLELLRDPNGLGW
                     ALAEHETHNERGESYLLSEVRLKSPIPEPPTLRDFYAFEQHVRAARFKRNAEMIPEWY
                     QIPTFYFSNTSEIYGHDEAVPYPVGSNELDIELEIACVIGREGKDIPVEEAAAYIAGY
                     TIMNDWSARDFQRLDMKLNLGPGKGKDFATSLGPWLVTPDELEERKSGSGASERYDMT
                     MLARVDGQEISRGNFKDIYYSFPQMIAYASRNVRLRVGDVIGSGTVGTGCLLEIGTGV
                     HPWFQRGEVIELEIDGIGVLRNTIV"
     gene            complement(2685..3788)
                     /gene="hppD"
                     /locus_tag="VGT44_15070"
     CDS             complement(2685..3788)
                     /gene="hppD"
                     /locus_tag="VGT44_15070"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEV2582176.1"
                     /translation="MPEQDFMPIQNYDYVEFYVGDAKQAAYYFTHAWGFTPIAYAGLE
                     TGVRDRASYVLEQGNIRFVITSTLEPQGLIADHVRLHGDGVKDIALRVDDAARAYREA
                     TTRGAKGVMEPTTRKDDYGVVKVAAIATYGETIHTFVERQDYKGVFLPGYRALDSDVP
                     RRARPAGLAAIDHIVGNVELGKMNEWVAFYERVMGFTQLIHFDDRAISTEYSALMSKV
                     MQNGSGRIKFPINEPAQGRRKSQIEEYLDFYRGPGAQHIALNTTDIVTTVRGLQERGV
                     EFLRTPYNYYETVLDRVGSIAEDVQQLAELSILVDHDDEGYLLQIFTKPIVTRPTVFF
                     EVIERKGARGFGEGNFKALFEAIEREQSLRGNL"
     gene            complement(4069..5208)
                     /locus_tag="VGT44_15075"
     CDS             complement(4069..5208)
                     /locus_tag="VGT44_15075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HEV2582177.1"
                     /translation="MPLYASPIGLEVAQAHIYDVIVVGAGPAGSSAAYHLASRGVDVL
                     LVDRFAFPRDKRCGDAVMSPSLEEMELMGLREETMERFEKIERIGMWLKDLPGSYQPV
                     EGLRGYVAPRVEFDAMLCEHALRHGAGWLDRLSVQEVAVEESKARLHGSHMGQPVSLQ
                     ARLVVAADGSGSRLARNLRASIDQQTDPTAIAADALTAPQDDRTRFTAMRGYYSGIEE
                     LTDALEFYFRAEAGTVYYWIFPCGQGLANVGVIASMAQLRATRTELEGALAAFMQSPE
                     LEGRALRARLKGRLGAAPIAAGLRGTVLYGERMLCVGDAAALVNPISAEGISGALWSG
                     RVAAETTADALARGDCSMEALSPYGEAVREKYQVMYDMLLSSFHV"
BASE COUNT         1063 a         1644 c         1507 g         1104 t
ORIGIN      
        1 gatcctcatc agacaggtga tccgccagcc aatctgtctc caccagggga tgcgctgaga
       61 aatcgatcat ccactcctcc ttatctggta atctccattc ctcttgtgct tgtatatcat
      121 atccgctaaa gaatacaacg attgaattgt gcatctgaat atccagtggg ctatcaagat
      181 tcaagcgaaa tgtaggggag caattcattg cgccctcttc ccaggccgat gcatgacgag
      241 gtgggcgcaa tgaattgctc ccctacattt ctccgttcca ttcaagtgcg tcagaccaca
      301 agataggggt agattcgctc ttgccaatcg tatggcgcaa accccacgct tgagacgatc
      361 atcgagttcc tttcagcatt acacctgacc gccgatatca ccctacgccc aacgagagag
      421 ggcgaggggc cgatgaaggt tgtgcttgaa acgcctgcta cataccagat ttcgcgcgag
      481 ggatgaagac gtttgcgtca cccgcgtatc tgctgttgac acgattcctt cgcacttacc
      541 aggtacgcgg gcgacgcaag cgtccccact ccgctccaca tcctctcccg cccctacggg
      601 ttattcgatc cagctatacg gatacgctgg atcatcaatg gtctgcgccg ccgaggtgta
      661 cttgagcggg tagaaggtat cgaccatcac ggccagctcc tcggtgcgct cggcccccag
      721 gctcttttcg accgcgccgg gatgcgggcc gtgcgcgatg ccgcgcgggt gcagtgtcat
      781 agacgagcgc tcgatgccgc gccggctgcc gaaattgccg ttgacgtagt agatcatctc
      841 ctcgctgtcg atgttgctgt ggttgtaggg aaccggaatc gaaagcgggt gatagtccag
      901 cttgcgcggc acgaaggagc agaccacgaa gttcggcccc tggaaggtct gatgcaccgg
      961 cggcggctgg tgaacgcgcc ccgtaatcgg ctcgaagtcc gcgatattga aaatccaggg
     1021 atactcgcag ccgtcccaac caaccgtatt gatggggtgg aagtcgtacc agtagctcga
     1081 tatgagattg ccgaccttga tgcgcacctc gaactcgccg cgctcggtat gcgttaccag
     1141 ttcctccggt cggcgaatat cgcgctcgca gtagggcgaa tgctcgagca tctggccgtt
     1201 cttgttgagg tagcggcgcg gcggctcgat tgtgccgcgc gcctcgatgg tcagaaagcg
     1261 cgattcggcg ctgtccgagg cgatctggaa acggaaggtc gtgccgcgcg gaatgacaat
     1321 atagtcgccc tcgtgatagg gcagcaggcc aaagttggtc tccaggatgc cgtgtccatc
     1381 atgcacgaag tagaccaggt cggagtcgcc gtcgcgatag aagtagttca tggcctgcgc
     1441 gggcagcgcg acgccgatgg ctacatcctt gttgtacatc atcacctgcg tccccgatac
     1501 agaatcgcct ccgcgccgca gattggccga tttgaggtgg tggtggcgct gcacctcctg
     1561 cggccattcc tccgtgagaa acgacacatc tccaaatttc tccaccttct tcacgcgcgt
     1621 cggtggatag gtatagtaga ggttagagta gttgcccgaa aagccctccg tgccgaacaa
     1681 ttcctctgaa tacagcgttc cgtctggttt gcggaactgc gtatgccgct tgtgggggat
     1741 ttcccctaaa cgatgatatg gaggcatgtc tttctccttt acacaatcgt atttcgcaat
     1801 acaccgatgc cgtcgatttc cagttcgatc acctcgcccc gttggaacca gggatgcacg
     1861 ccggtgccga tttcgagcag gcagccggtg ccgacggtgc cggaaccaat cacatcgcca
     1921 acacgcaggc gaacattgcg cgaggcatag gcgatcatct gcgggaagct gtaataaatg
     1981 tccttgaagt tgccgcgcga aatctcttga ccgtccacgc gcgccagcat agtcatgtcg
     2041 tagcgttcgc tcgcgccgct gccgctcttg cgctcctcaa gctcgtcggg cgtgaccagc
     2101 caggggccga gcgaagtggc gaaatccttg cccttgccgg gacccaggtt gagcttcata
     2161 tccaggcgct ggaagtcgcg cgcgctccag tcgttcatga tcgtgtagcc cgcgatatag
     2221 gcggcagcct cttccaccgg gatatcctta ccctcgcgcc cgatcacgca agcgatctcc
     2281 agttcgatat ccagctcgtt gctgccaacc gggtacggca cggcctcgtc gtggccgtag
     2341 atttcactgg tgttactgaa gtagaaggtc ggaatctggt accactccgg gatcatctcg
     2401 gcgttgcgtt tgaagcgcgc cgcccgcaca tgctgctcga aggcataaaa atcgcgcaag
     2461 gtcggcggtt cgggaatggg cgacttgagc cgcacttcgc tgagcagata gctctcccct
     2521 cgctcgttgt gcgtttcgtg ttctgcaagc gcccagccta aaccgttggg gtcgcgcagc
     2581 agttccagca cgtctgggta gtcgagcgcg ataaccacgc catcgcgcag cacacccgcc
     2641 tgtgtccgat gagattttgg cgcaataaac gtaaccagtc tcatctacag attcccccgt
     2701 agcgactgct cgcgctcgat agcctcgaaa agcgccttga agttgccctc gccgaaaccg
     2761 cgcgctccct tgcgttcgat cacctcgaag aagacggtcg ggcgcgtcac aatcggtttt
     2821 gtgaagattt gcagcagata gccctcgtcg tcatgatcga ccaggatact caactcggcc
     2881 agttgctgca cgtcctcggc gatgctgccg acgcgatcca gcacagtctc gtagtagttg
     2941 tagggggtgc gcaggaattc taccccgcgc tcctgcaggc cgcgcacggt ggtgacgata
     3001 tcggtggtgt tgagcgcaat atgctgcgct ccggggccgc gatagaagtc gaggtactct
     3061 tcgatctgcg atttgcggcg gccctgggcg ggttcgttga tggggaattt gatgcgcccg
     3121 ctgccgttct gcatcacttt tgacatcagc gcggaatact cggtgctgat cgcccggtca
     3181 tcgaagtgga tcaactgcgt gaagcccatc acgcgctcgt agaaggccac ccattcgttc
     3241 attttgccca gctcgacatt gccgacgatg tggtcgatgg ccgccagtcc cgccgggcga
     3301 gcgcggcgcg gcacatcgct atcaagcgcg cgatagccgg gcagaaagac gcccttgtaa
     3361 tcttgccgct caacgaaggt gtggatcgtc tcgccatacg tcgcgatggc ggcgaccttg
     3421 accacgccgt aatcatcctt gcgcgtagtc ggctccatca cgcccttcgc gccgcgcgtg
     3481 gtggcttcac gataggcgcg ggcggcatca tcgacacgca gcgcgatatc cttgacgcca
     3541 tcgccgtgca gcctgacatg atcggcaatc aagccctgtg gctccagcgt ggacgtaatc
     3601 acgaatcgaa tgttaccctg ctccagcaca tagctggcgc ggtcgcgcac gccggtctct
     3661 aacccggcat aggcgatagg ggtaaagccc caggcgtggg taaagtaata ggcggcctgt
     3721 ttcgcgtcgc ccacgtaaaa ttcaacgtag tcgtaattct ggatcggcat gaaatcctgc
     3781 tcaggcatat gcggctctcc ctttgtcttg tttccttgaa aacactcttc cacaaaagac
     3841 gcaggagcag gggaaggcag gatgtggcaa ccatcccgaa actcctttgt ttcgatacgg
     3901 cgcaatgaca cgtttgtctg ctgtcattat atgtgatgcc agggaagcgc cgcaaggcaa
     3961 cgcctctcaa cgagcgtata aattccacgt gtagtttgtc atcctgagcg cagcgaagga
     4021 tctcggtccc tggccagtga gatccttcgc tgcgctcagg atgacaaact acacgtggaa
     4081 tgaactcagc agcatatcat acataacctg gtatttctca cgtaccgcct cgccataggg
     4141 actcagcgcc tccatcgagc aatcacccct tgcaagggcg tccgcagtcg tctcggcagc
     4201 gacgcgcccg ctccagagcg cgccggagat gccctcggcg gagatagggt tgaccagtgc
     4261 ggcagcgtct ccaacgcaga gcatccgctc gccatacaac acggtcccgc gcaggccagc
     4321 cgcgatgggg gccgcgccca gccgcccttt aagtcgcgcc cgcaacgctc gcccttcaag
     4381 ctctggggat tgcatgaagg cggccagcgc gccctccagc tcggtgcgag tcgcgcggag
     4441 ctgcgccatg ctggcgatca cgcccacgtt ggcgagtcct tgcccgcaag gaaatatcca
     4501 gtagtagacc gtcccagctt ccgcgcgaaa atagaattcc agcgcgtcgg taagctcctc
     4561 gatgccgctg tagtagccgc gcatggccgt aaagcgcgtg cgatcatcct ggggcgcggt
     4621 cagagcatcc gccgcgattg ccgtcggatc ggtctgttga tcgatgctag cacgcagatt
     4681 tcgcgccagt cgcgatccgg agccgtcagc cgcgacgacc agccgcgcct gcagcgacac
     4741 tggctgcccc atatggctcc catgcaggcg cgccttgctc tcttctaccg cgacttcctg
     4801 cactgacagc cggtcgagcc agcccgcgcc atgtcgcagg gcatgttcgc agagcatggc
     4861 atcgaattcc acgcgaggcg cgacgtatcc cctcaaaccc tccactggct gatagctgcc
     4921 gggcaggtcc tttagccaca tgccgatacg ctcaatcttc tcaaaacgct ccatcgtctc
     4981 ttcgcgcagg cccatcagtt ccatttcctc gagcgacggc gacatcacgg catccccgca
     5041 gcgtttatcg cgcggaaacg cgaagcgatc cacgagcaga acatcaactc ccctgctggc
     5101 caggtgatac gcggcagacg atcccgccgg cccggctccc acaacgatca catcatagat
     5161 atgcgcctgg gccacctcca gaccgattgg ggatgcatag agtggcatgc aggtaccttc
     5221 cttgttagcc gttttgcatc aaacacatcc aaattattca aagtataaca tgtcgagccg
     5281 ctggttccga gggcgaccgc aagggtacgc ccttacaa
//
LOCUS       DASZAK010000855         2783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_413592, whole genome shotgun sequence.
ACCESSION   DASZAK010000855 DASZAK010000000
VERSION     DASZAK010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2783
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413592"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VGT44_15080"
     CDS             complement(<1..843)
                     /locus_tag="VGT44_15080"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEV2582178.1"
                     /translation="MTNYIDLHTHSTASDGIYSPTELLHKAKEAGLRVLALTDHDTTG
                     GLEEAARAARTLDIDLIPGIEINTDVSGGEVHVLGYFIEFQRPEFQAVLKVLRDARER
                     RGQRMVELLNEHGVNIAWDRVREIAQGSVGRPHVAKALQEAGYVQTIGEAFDKYIGNG
                     CYAYVPRYKLTPEDAVHLVASANGLPVIAHPLELPGLAVLREWLPGLCEAGMAGLETY
                     YGLYTPENERELLALAREYDVIPTGGSDFHGPGLHPTPLGGRYVPFEAVERLKAEAAK
                     RRGKT"
     gene            complement(876..2429)
                     /gene="rny"
                     /locus_tag="VGT44_15085"
     CDS             complement(876..2429)
                     /gene="rny"
                     /locus_tag="VGT44_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV2582179.1"
                     /translation="MQSTEIVIALIILVFGAGVALIAGYVIGSTRNKQYFAERSRVEK
                     EESEQKLMELQMQQREALHEARDETARFRAAMEKDNAERRAELQRQERRLQQKEETLE
                     RKIDALEQRERKLVARERSLEQTQEELEVKKREQLKELERIAHLTEEQAKEVLLSRIE
                     DQVRIEAARRIRAIEEEAREEADARAREIITLAIQRCASDQVAEAVVSVVPLPNDEMK
                     GRIIGREGRNIRALEAATGVDLIIDDTPEAVILSGFDPVRREVARVALTKLIIDGRIH
                     PARIEDVVAKARQEVDAIVREEGERAAMEANVHGLQPELLKILGRLHFRTSYGQNVLA
                     HSVEVSILAGTIAHELGADVNVCKTAALLHDMGKAIDQEVEGPHAIIGGEIARRFGKS
                     PKVIHAMVAHHATESEPQSLEAAIVQAADAISAARPGARRETIDLYIKRLEALEYIAN
                     SFTGVEKSFAIQAGREVRIIVKPEEIDELSANRLASDIAHKIEESLDYPGQIKVCVVR
                     ETRSIDYAR"
BASE COUNT          603 a          778 c          712 g          690 t
ORIGIN      
        1 tgtcttgccg cgccgtttgg ctgcctctgc tttgaggcgc tctactgcct caaatgggac
       61 atagcgacca cccagaggcg tcgggtgcaa acccgggcca tgaaaatcgg accctcctgt
      121 agggatcaca tcatattcac gagccagcgc aagcagttcg cgctcgttct cgggtgtgta
      181 aagtccataa taagtttcca gccctgccat gccagcttcg cacaaaccag gcagccattc
      241 acgcagtaca gcgagtcccg gaagttccag gggatgggcg ataacgggta agccgttagc
      301 gctggcgacg agatggactg catcttctgg agtgagcttg tatcgcggga catatgcata
      361 gcaaccattg ccgatatact tgtcaaaagc ctcgccaatg gtttgcacgt aaccagcctc
      421 ctggagcgct ttggcgacgt gcggtcggcc tactgacccc tgagcgatct cacgcacgcg
      481 atcccaggcg atgttaacgc catgttcatt caatagctcg accatacgtt ggccacgccg
      541 ctcgcgcgca tcacgcagca ctttgagtac agcctggaac tcggggcgct ggaattcgat
      601 gaagtagcca agcacatgca cttccccgcc gcttacatcg gtgttgattt caatgccggg
      661 gataaggtct atatctaacg ttcgtgccgc tcgcgcggct tcttcaagcc ctccggtggt
      721 atcgtggtca gtcaaagcca gcactcgcag cccagcttct ttggctttat ggagcaattc
      781 cgttggcgag tatatgccat cggaagctgt ggaatgcgta tgcaggtcga tataattcgt
      841 catgtacaat gttccaattc tacgttgatc ttcgattacc tggcgtaatc gattgagcga
      901 gtctcgcgga cgacacacac cttgatttgt ccagggtagt cgaggctttc ttcaatttta
      961 tgagcgatat cacttgcgag acgattggcc gataactcat caatttcttc gggcttaacg
     1021 ataatacgta cctctcggcc tgcctggatc gcgaaggatt tctctacgcc ggtaaaagag
     1081 tttgcgatat attccagcgc ctctaaacgc ttgatgtaca ggtcaatggt ttcgcgacgc
     1141 gcgccaggtc gagcggctga aattgcatcc gccgcctgta cgatggccgc ttccaggctc
     1201 tgaggttcgg attcagtagc atggtgagct accatagcat ggatcacctt gggcgatttg
     1261 ccgaaacgac gcgctatctc gccaccgata atcgcatgag gcccctcgac ttcctggtcg
     1321 atagccttac ccatgtcgtg caggagcgct gcggttttac agacattcac atcggcgccc
     1381 agctcgtgag caatcgttcc tgcgagaatg gagacctcta ctgaatgagc gagcacgttc
     1441 tgtccataac tggtgcgaaa atgaagccgt ccaaggattt tgagcaactc tggctgcaag
     1501 ccatgcacgt tggcctccat ggcagctcgc tcgccctcct cgcgcacaat agcatccact
     1561 tcctggcgcg ctttggcgac aacatcctca atacgggcgg ggtgaatgcg cccgtcgatg
     1621 atcaatttgg tcaatgcaac acgcgccact tcacgacgaa cagggtcgaa gcccgaaagg
     1681 attactgcct ctggagtatc atcaatgatc aggtcgacac ctgtcgcagc ttccaacgcg
     1741 cggatattgc gtccttcgcg tccgataatg cgccccttca tttcgtcgtt cggtaatggc
     1801 acaactgaga ctactgcctc cgcgacctgg tctgaggcgc accgttgaat cgccagggta
     1861 atgatctccc gcgcacgcgc atccgcctcc tcacgcgcct cctcttcgat agcgcgaatg
     1921 cggcgggctg cttctatacg cacctgatcc tcgatacgtg agagcaagac ttctttggct
     1981 tgctcctcgg tgagatgagc gatgcgctca agttctttca gttgctcgcg ttttttgact
     2041 tccagctctt cttgtgtttg ctcgagtgat cgttcccgag ctacaagctt gcgttcacgc
     2101 tgctccaacg cgtcaatttt gcgctcgagc gtctcttcct tttgctgcaa gcgacgttcc
     2161 tgccgctgta actcggctcg ccgctccgcg ttgtcctttt ccatagctgc acgaaagcgc
     2221 gctgtttcgt cgcgcgcttc atgcaaagcc tcgcgctgct gcatctggag ttccatgagc
     2281 ttttgctcac tttcctcttt ttcaacacgc gaacgttctg caaagtattg tttatttcgt
     2341 gtcgacccga taacgtatcc agcaatgagg gcaactccgg ctccaaaaac caggataatc
     2401 agggcgataa cgatttccgt tgactgcacg ttggcctacc tccaatagta aaagcgaaaa
     2461 ggcctttaag ggtgtaaaag gagtatacta ctcaaatagg tatgctaaat ggcttagtgc
     2521 caaaagtatg ctattcgcca agcatactat ataggagtgg tatctatgct gtcaagcata
     2581 gcgtgatagc ctgtttatca taagtttggg gaggaatcaa catggatcaa aaacaaccag
     2641 ggcaaagttc tcagcagccg ttacgacaaa cgcatgttgt ctcctgtttt cttttgcgaa
     2701 aagatatgga ccagcctcgc atgctaattg tgcgaaggag ccaacgcgtg gggagctatc
     2761 atgctcgttg ggcaggtatc agc
//
LOCUS       DASZAK010000856         1500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_22162, whole genome shotgun sequence.
ACCESSION   DASZAK010000856 DASZAK010000000
VERSION     DASZAK010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22162"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1211)
                     /locus_tag="VGT44_15090"
     CDS             complement(150..1211)
                     /locus_tag="VGT44_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2582180.1"
                     /translation="MVARATKTYRVGNVDNGGSVDRGAHKGVALAEKPGWTRSRKAKD
                     ERDPELDMQDAFAFEDEEDWGEDSDQSDHQGAADDAVKQYLQEIGTFPLLTAEQELQL
                     AERIAHGDLRARQKLIEANLRLVVSIAKRYTNQGLPLLDMVQEGNFGLMRAAQKFDYQ
                     RGFRFSTYATWWIRQAISRAIAEHSRTIHIPVHVVEYIYKIKRVARRIYQEEGIEASP
                     EQIAAEVGLSKERVIELLNVSDQPISLDAPVADDEQYHLADAIEDTTLSTPAEEVSQR
                     MLREYINQALAILNPRERTILEMRYGLQDGHTHSLDEVSAVYHLTRERIRQIEVKALR
                     KLRYPDRYCDVGLQGYVQA"
BASE COUNT          280 a          418 c          445 g          357 t
ORIGIN      
        1 ttgtaagggc gggggtggag cggagtgggg tggagaccct tgcggtcgcc ctcgtactcc
       61 ccgctctgtt ggtcgaagta tttcgttcat ctgcattagc ggccacgatg gacatgaacg
      121 gcaagcaccc tgctcctaca agctcatcat tacgcctgca cgtacccctg caagcccacg
      181 tcgcagtagc ggtccgggta gcgcagcttg cgcagggctt tgacctcgat ctggcggatg
      241 cgctcgcggg tgaggtggta gacggcgctg acctcgtcca ggctgtgggt atggccgtct
      301 tgcaggccgt agcgcatttc gaggatggtg cgttcgcggg gattgaggat ggccagggcc
      361 tggttgatgt actcgcgcaa catgcgctgc gacacctctt cagccggtgt tgacagggtg
      421 gtatcctcga tggcgtcggc gaggtgatat tgttcgtcgt cggccaccgg cgcgtcgagc
      481 gagatgggct gatcggagac gttgagcagt tcgatgacgc gctctttgga gagacctacc
      541 tcggcggcga tctgctccgg cgaggcttcg atgccctctt cctggtagat gcggcgcgcc
      601 acgcgcttga tcttataaat atattccacg acatgcaccg gaatgtggat ggtgcgcgaa
      661 tgctcggcga tggcgcggct gatggcttgg cgaatccacc aggtcgcgta ggtgctgaac
      721 cggaagccgc gctggtaatc gaacttctgg gcggcgcgca tcaggccaaa attgccttcc
      781 tgcaccatat ccagcagggg cagcccttga ttggtatagc gtttggcgat gctgaccacc
      841 agtcgcagat tcgcttcgat cagcttctgg cgggcgcgca ggtcgccatg cgcgatgcgt
      901 tcggccagtt gcaattcctg ttcggcagtg agcaaaggaa acgtgccgat ttcctgcaaa
      961 tattgtttga ccgcatcatc ggcagcgccc tggtggtctg actgatctga atcttctccc
     1021 cagtcctcct catcctcaaa tgcaaaggca tcttgcatat ccagctcagg atcgcgttcg
     1081 tctttcgcct tgcgagagcg cgtccagccg ggtttctccg ccagggccac gcccttatgc
     1141 gctccacgat ccacactccc accattatct acatttccaa cacgatatgt ttttgttgct
     1201 cttgctacca tctgtcatac tccttttgca tattgaatga tctcctaccg tccaatactg
     1261 caactatcgt gccaaacggc ttgagacgca tcctctatca atacatttca tcgtagttca
     1321 atctgtagcg cctatattca tcggcttgca ggctcgattt ctgaagggta atcagggaaa
     1381 gaacaggcga ggaatcgcca caggctctct catgcggtgg tcaaccatcg gctgaagatg
     1441 gaagggattt cagatagtgg ggaattttgc gcaggtgggg catggcgacc gcaagggtcg
//
LOCUS       DASZAK010000857         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_341368, whole genome shotgun sequence.
ACCESSION   DASZAK010000857 DASZAK010000000
VERSION     DASZAK010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341368"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VGT44_15095"
     CDS             <1..230
                     /locus_tag="VGT44_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582181.1"
                     /translation="TAISSRVNDTRTAQQLSIWAVVPIAGIILGELSGQFELTVLVAV
                     IAIGILIPLAILLTWGAAQLFQRETILTRWK"
     gene            810..1364
                     /locus_tag="VGT44_15100"
     CDS             810..1364
                     /locus_tag="VGT44_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2582182.1"
                     /translation="MHIVDLNPADQPAIQQVATLLVEGFATNWPDAWPDMESALEEVR
                     ESFAADRISRIALDEDGTVLGWVGGISQYRGRVWELHPLVVRVSQQGKGIGRALVADL
                     EERVKERGGLTITLGTDDVNHQTSLSGIDLFPNVWEHVAHVRNLKRHPYEFYKKQGYV
                     ITGVMPDANGLGKPDILMAKSLVR"
     gene            complement(1379..1738)
                     /locus_tag="VGT44_15105"
     CDS             complement(1379..1738)
                     /locus_tag="VGT44_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582183.1"
                     /translation="MLSDSYKTTITDELMRQRISATRQYCIVILKAGPGKNEEGADKI
                     IWEHGRRNFALREAGLLSIVCPIADGTNVSGVGIFNATVEEVNKIMDEDPAVQAGVLI
                     YETHACRGFPGDSLPKE"
     gene            complement(1996..>2654)
                     /locus_tag="VGT44_15110"
     CDS             complement(1996..>2654)
                     /locus_tag="VGT44_15110"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEV2582184.1"
                     /translation="ENSQAHTSIMNYPFLSGVLALDLVNTEVIVRGRRYDLLCSPEDV
                     ARWWQEALAHHPDREKVKEETQATAWSLPLLEKIKQVRAAIRTLCSNLVQQQPLDQEA
                     LAVLNAILAMGCPALDVAAEGEMVPVYRAREADQGAILMTIALSAFHLFTQAEKHRLH
                     KCKNDRCILFFYDNTKSATRQWCSLECMNRARSLQHYQHVKEERLRQTGKVLRPKAVL
                     "
BASE COUNT          603 a          672 c          701 g          678 t
ORIGIN      
        1 tgacggccat ttcgtcgcgg gtcaacgata cccgcacggc gcaacaactc tcgatatggg
       61 cggtggtgcc catcgcgggg atcattctcg gcgaactctc cggccagttt gaactcaccg
      121 tcctcgtggc ggtcattgcc atcggtatcc tgatccctct cgcgatactc ttgacctggg
      181 gcgctgccca actgtttcag cgcgaaacca ttctgactcg ctggaagtag tagcgtgtta
      241 caagggtcag catatccaag agcagattgg atatgccgac cccttccatg tcatatgcag
      301 gcgctcgcgc aggccgctcc acgatacctc gtgtttcgtc tggaccgttc aaatgccccg
      361 aaccggacac ccctgcgagc gcggaacggg caactgtgtc ccttatggac aaggtctccg
      421 ctgttctaac gaggctgacg aaaggccaaa tcgcttgtgg atatctcgca cttcaggggc
      481 tttttgaggg cttagcgttg taaatttctc cggcgggcct aataggccat tactggtttt
      541 gccgtgccga ttgtactaca gttcgcttaa aaagcatata gtcttgataa ctgcccggag
      601 ctcttgaagc tttacctgca cttcttttgg ggcttgtgca atatattcat ccacattttt
      661 tttgcaggtt tcatatgcta tgctcctttt gagacagaaa ctagcagcca gaagaagtat
      721 acagtatcat caggacgtct gtacaggtag cagtagtgcg aactttgtgg gccaaataag
      781 cataaacagg atactcaagg aggacagcta tgcacattgt tgatcttaat cctgctgatc
      841 agccagctat ccagcaggtt gctactttgt tagtagaggg ctttgcgact aattggccgg
      901 atgcgtggcc tgatatggaa tctgctctgg aggaagtgcg cgagtcgttt gcggcagacc
      961 gcattagcag gatagctctt gatgaagacg gaacggtact cggctgggtc ggcggcatta
     1021 gccagtatag gggccgcgtg tgggaactgc atcccctggt ggtgcgtgtc agccagcagg
     1081 ggaaagggat cgggcgtgcg ctggtggccg acctggaaga gcgggttaaa gagcgtgggg
     1141 gattgacgat tactcttggc accgatgatg taaatcatca gaccagcctt tccggtatcg
     1201 atctctttcc caatgtgtgg gagcacgttg cccacgtcag aaacctgaag aggcatccct
     1261 acgagtttta caagaagcag ggttacgtga ttaccggcgt aatgccagat gcaaatggac
     1321 ttggcaagcc agatatcctt atggccaaat cgcttgtgag atagaagaaa tcatcctgtt
     1381 attccttcgg taagctgtct ccgggaaaac cccggcaagc atgcgtttca tagatcaaca
     1441 ctcctgcctg tacagcaggg tcctcatcca tgatcttatt gacctcttcg acagtcgcgt
     1501 taaaaatgcc cacaccggag acattagttc catcagcgat cgggcataca attgataaca
     1561 atccagcctc ccggagggca aaatttcttc gtccgtgttc ccagatgatt ttgtcggctc
     1621 cttcttcatt tttccctggt ccagccttca aaatcacgat gcaatactgc ctggtagcgg
     1681 atattctttg ccgcataagc tcatcggtaa tggtggtctt gtaggaatcc gaaagcatct
     1741 tttatctcct tctgtatttt attttatgga aacattaaca aataaagtga gacatcggga
     1801 acccccaccg aaggccgata aatcggcggt gggcacgata aatcggcccc tacgaatgtc
     1861 tggattcatt tgtaaaaagt cataagataa agtgaaaaca gaacgtttcg ttcagacata
     1921 gggggatttc gtgtgctggc aagtcctccc tcgtcttatt ctaatctgga tagatcgaaa
     1981 cggaaagatg gcaccttata acacggcttt cggacgcaga accttgccag tttgacgcaa
     2041 tcgttcctct ttcacgtgtt gataatgctg caaagagcgc gctcgattca tgcattcgag
     2101 gctgcaccac tgacgcgtgg cgcttttggt gttgtcatag aagaataaga tgcagcggtc
     2161 attcttacat ttgtgcaggc gatgtttctc ggcctgggtg aacagatgaa acgcggacag
     2221 ggcaatggtc atgaggatgg ctccctggtc ggcttcacgt gcgcgataaa cggggaccat
     2281 ttctccttct gcggcaacgt ccagggcggg acagcccatg gcaaggatgg cgttgagcac
     2341 agcaagcgct tcctggtcaa gcggctgctg ctgcaccagg tttgaacaca atgtcctgat
     2401 agctgcccgg acctgcttga ttttctccag gagtggcaga ctccacgcgg tggcctgcgt
     2461 ttcttccttg accttctccc tgtcaggatg gtgcgcgaga gcttcctgcc accagcgtgc
     2521 cacatcctcg ggtgagcaga gcaagtcgta tcttcttccc cgaacgatca cttctgtatt
     2581 gaccaggtca agcgctaaca ccccgctgag gaatgggtaa ttcattatac tggtgtgtgc
     2641 ctggctgttt tcgt
//
LOCUS       DASZAK010000858         2574 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_164263, whole genome shotgun sequence.
ACCESSION   DASZAK010000858 DASZAK010000000
VERSION     DASZAK010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164263"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..2179)
                     /locus_tag="VGT44_15115"
     CDS             complement(104..2179)
                     /locus_tag="VGT44_15115"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEV2582185.1"
                     /translation="MTTQDTQSDYDDARVNSAEQSSLSHAPTQDSGAAPSNGETYPSL
                     NALEAHPLTLQPSDDMIARPFTEYFEEFRRSETFPSAAQGESTNPSNQANEGVVIDAS
                     PSSVGTGQSEEGSPVLSPALPVERPVSPLLRPASRIRVPKHGNRHRQEPVAKPAQDPA
                     SADATSDQQENASVAQETQTDKSSPAQEQQETQRPPRRYRFDRPAPTAVQAPANAHVV
                     PQIGKSAQVSSTRVEQGQNGNRETRDASRVVPNGTEGDLQPAQASQAAQAPVENELSR
                     PNVQHHPRQQQGHRPERTQKAQDAVLPTGAGTPETTVITPAPEIGQGDSTTADIALED
                     LPPLEYADLQAATSRRRRRRRVNAPAPAITPAAPGSPTTAITPIPTTAAAPQLQNVPT
                     APQSHNAPVSPITPVVSVTPFSIQSGATLNQMNQGGEARGPFMGPEPSPARGSVMQSS
                     LRPLRTGRNMNNGDAQRGIVPAVPGTPVRPGEGIISNGGVNYLANVLKDAIQTQTDRI
                     AAELRRSSQTPTSVTVSMPPFPSTERVGVFVDVANLLYSARNMRINIDFGKLLDFLRG
                     NRRLVRAQAYCPTSPQPGEDQMFLQAVKGLGYRITTKNYKTFASGARKADLDLDLCMD
                     VVRLVDGKAVDSIVLVSGDSDFMPMLDYCSDHGVRVEIAAFDESMSATLRQSCDLFVN
                     LAMLEEIRA"
BASE COUNT          459 a          694 c          813 g          608 t
ORIGIN      
        1 gcttctcaca gaagctgaga atgttcaacc acctaaaacg gcgtatgatg gcaacccgcg
       61 ggttgccatc atacgccgtt ttatgtggtt gaacattgat cctttatgcg cgaatctctt
      121 cgagcatggc gaggttgacg aacaggtcgc agctctggcg caaggtggcg gacatcgact
      181 cgtcgaaggc ggcgatttcg acgcgcaccc catgatcgga acaatagtcg agcatcggca
      241 tgaagtcgct gtcaccgctc accagcacga tgctgtcaac agctttgcca tcgaccaggc
      301 gcactacgtc catgcacagg tccaggtcca ggtctgcttt acgcgcgcca ctggcgaagg
      361 ttttgtagtt cttggtggtg atgcgatagc caagaccctt gactgcttgc aggaacatct
      421 gatcctcacc cggctgaggg ctggtgggac aataggcctg cgctcgcaca aggcggcgat
      481 tgccgcgcag aaagtcgagc aatttgccaa agtcgatgtt gatgcgcatg ttgcgcgccg
      541 agtagagcag gttcgcaacg tctacaaaga cgccgacacg ctcggttgag gggaaaggcg
      601 gcatcgagac ggtgacgctg gttggcgtct ggctggatcg ccgcagttcg gcagcgatgc
      661 ggtcggtctg tgtctggatg gcatctttga gtacattcgc caggtaattg acgccaccgt
      721 tagagatgat gccttcgccg ggtcgtaccg gtgttcccgg cacggcgggt acgatccctc
      781 gctgcgcgtc gccgttgttc atattgcgcc cggtgcgtag tgggcgtaga gacgactgca
      841 tgacgctgcc acgcgcgggc gacggctctg gccccatgaa tggtcctctt gcctcaccgc
      901 cctgattcat ctggttcagg gtcgcgccgg actggatcga gaacggcgtc acggatacaa
      961 ctggtgtgat cggcgagacc ggtgcattgt gcgactgcgg tgctgtcggc acgttttgca
     1021 attgcggcgc ggcagctgtc gttggtatgg gcgtgatagc tgtagtaggt gagccgggtg
     1081 cagcaggtgt aatggcgggc gctggagcat tgacgcggcg gcggcggcgg cggcgcgatg
     1141 tagcagcctg caaatcggca tattccaatg gcggcaggtc ttcaagcgca atatccgctg
     1201 ttgtagaatc accctggccg atttccggtg ctggtgtgat tactgttgtt tcaggcgtgc
     1261 ccgcgccggt gggcaacacc gcatcctgcg ccttttgcgt tctctccgga cgatgtcctt
     1321 gctgctgtcg cggatgatgc tggacgttag gccgggacag ttcgttttcg accggggcct
     1381 gtgctgcttg tgacgcctgg gcaggctgta gatcgccttc tgtcccattt gggacgactc
     1441 tgctggcgtc tctcgtttcc ctgttgccgt tctgcccttg ctcgacccgg gttgagctga
     1501 cctgggcgga tttgcctatc tgagggacga catgtgcgtt cgcgggagcc tggacagcgg
     1561 tgggcgctgg ccgatcaaag cggtagcggc gtggtgggcg ctgcgtttct tgctgctctt
     1621 gagccggtga agacttgtcg gtctgcgttt cctgcgctac agaagcgttc tcttgctgat
     1681 ccgatgtggc atcggcgcta gccgggtcct gagcgggttt ggcgacaggt tcctggcgat
     1741 ggcggttgcc gtgtttcggc acacgaatgc gcgatgccgg gcgcagcagc ggcgacacgg
     1801 ggcgttcaac cggaagggcg ggggatagga ccggcgatcc ttcctcgctt tgcccggtcc
     1861 cgaccgaaga gggtgaggca tcaatcacaa caccttcatt tgcctggttc gagggattag
     1921 tcgactctcc ctgcgctgcg gacggaaacg tttcgctgcg ccggaattcc tcgaagtatt
     1981 cggtgaaggg acgcgctatc atatcatccg atggctgcaa ggtaagcgga tgcgcttcca
     2041 gcgcgttcaa ggatggatag gtttcaccgt tggatggtgc tgctccagag tcttgtgttg
     2101 gagcatggga aagtgacgac tgctctgcac tatttacgcg agcgtcgtca taatcagatt
     2161 gtgtgtcttg tgttgtcact aagatctcca gtctcagtag gtactgaccg gagcgcatag
     2221 gctagcaatc gatagtagcc aggaagccag tgagaatatg tacttattca ctcatacgat
     2281 aacgcgacaa gcggcgcggc atttgcttta cacgcgcgtc aagcgcagat gacggtggcg
     2341 atttgtattg tacgccagat catgtgatcg tctcgcccga aaaacgggag tcctgatcta
     2401 ttctcggaca ggctctactt tgcttcttct acggtccagt ccgacctttt ctctacttgt
     2461 ctatgccgtt tcccttactc ttgctgtaca ctcccactaa acggcggaac actggtgcgt
     2521 acaggctgaa ttgaaggttc tataccttca tcggtgccaa ccgacccctg ccgg
//
LOCUS       DASZAK010000859         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_449703, whole genome shotgun sequence.
ACCESSION   DASZAK010000859 DASZAK010000000
VERSION     DASZAK010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449703"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VGT44_15120"
     CDS             complement(<1..398)
                     /locus_tag="VGT44_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582186.1"
                     /translation="MMTMTVQHVTYIPRFLPYEDFWALAERFCARRHHSKKSAAKQFQ
                     TLLCVLETFHQDHLAAMTTSGRALEELQEADLWFEVIWSALAHRAQYFCCESTFRMTL
                     PRLIDAGYVRQRYVKRDGGLVLAYATYKEA"
     gene            complement(565..972)
                     /locus_tag="VGT44_15125"
     CDS             complement(565..972)
                     /locus_tag="VGT44_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582187.1"
                     /translation="MPMQPDLYPANWKQLAESLKEQANWTCERCGAQRGQIVKNRHGK
                     EIPVVITVAHPNHDPRNPQARLAVWCAQCHCRYDAADRRRQRVMMRIARGQGVLPGLE
                     EWYMPPRKGGQRRKRERRAKNNLVYCGRSTRLP"
     gene            complement(995..1303)
                     /locus_tag="VGT44_15130"
     CDS             complement(995..1303)
                     /locus_tag="VGT44_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582188.1"
                     /translation="METIDPIPLPWWQLPSERFAAWRRSVLDRWDAPVVEADDPSVRG
                     PAWRGWDPEATQYSLTRVMESIATTEVEIEAATEPAIGVITEKLPRRWSWRRWRLWRT
                     "
     gene            complement(1290..>1579)
                     /locus_tag="VGT44_15135"
     CDS             complement(1290..>1579)
                     /locus_tag="VGT44_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582189.1"
                     /translation="EQALDYLDQAERQFAQRCTYPGCTIEASTDECAGFLVNAADGEL
                     HRQNCWPFVGRLCLAHMRQVVVPPDWWISETALSPAGFGSDLEKVGAGGDD"
BASE COUNT          319 a          490 c          423 g          347 t
ORIGIN      
        1 tgggcctctt tgtaggtcgc gtaggcgagc accaggcccc cgtcgcgttt cacatagcgc
       61 tgccgaacat atccggcatc gatgaggcga ggaagcgtca tacggaaggt gctctcgcaa
      121 caaaagtatt gcgcccggtg cgccagcgct gaccagatca cctcaaacca gagatctgcc
      181 tcctggagtt cctccaacgc gcggcctgat gtggtcatgg ccgcaagatg atcctggtga
      241 aaggtctcca gcacgcagag cagcgtctga aactgcttgg ctgcactttt ttttgagtgg
      301 tgccgccgtg cgcaaaaacg ctcggccaac gcccagaagt cttcgtaggg caggaaacgt
      361 ggaatatatg tcacgtgttg cacggtcatg gtcatcataa aaacacccct tgctataccc
      421 ggaacaaatg gccttctggc caatgacaag catctggcac gagttgccaa agcacttgcg
      481 ttgatacgca gcgcatgtta cactgcgtat caaagagaaa atgagttggt tttggtggca
      541 ttcctgagcc actcgcaaga cagatcacgg tagcctggtc gatctaccac aatagaccag
      601 gttatttttt gccctccttt ctcgctttcg cctttgccct cccttgcgcg gcggcatgta
      661 ccactcttcc aggccgggga gaactccctg cccacgcgca atgcgcatca tcacgcgctg
      721 acgccgcctg tctgctgcat catagcggca gtggcattgt gcgcaccaga cggcgagccg
      781 ggcttgcgga ttgcgtgggt catgattggg atgagcgacc gtgataacca ctggaatctc
      841 tttcccatgt cgatttttga caatctggcc gcgctgggct ccacatcgct cgcacgtcca
      901 attcgcttgt tctttgagcg attccgcgag ctgtttccag ttcgctggat acaaatcagg
      961 ttgcatgggc attgtggtac tcctttccgt cgatctatgt gcgccagagc ctccatcgtc
     1021 tccaactcca gcgcctgggt aatttctctg tgataacccc gatcgccggt tctgttgcgg
     1081 cttcgatctc cacttcggtc gtggctatcg actccatgac gcgcgtgagc gaatactgcg
     1141 tcgcttccgg gtcccatccg cgccaagcgg gacctcgtac actcggatca tccgcctcga
     1201 caactggcgc atcccagcga tctaagaccg agcgccgcca ggcagcaaag cgttctgatg
     1261 gcagttgcca ccagggcagt gggataggat caatcgtctc cacctgcccc cactttttcc
     1321 aggtcagatc cgaaacctgc cggagacagc gctgtctccg aaatccacca gtcaggtggc
     1381 acaaccacct gcctcatatg cgccaggcac agccgaccaa caaatggcca gcagttctga
     1441 cgatgaagct cgccgtccgc cgcgtttact aggaagcccg cgcactcgtc cgtcgaggcc
     1501 tcgatggtgc aacccggata ggtgcagcgc tgcgcgaact gccgctcggc ttgatccagg
     1561 taatcgagcg cctgctcac
//
LOCUS       DASZAK010000860         4791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_627428, whole genome shotgun sequence.
ACCESSION   DASZAK010000860 DASZAK010000000
VERSION     DASZAK010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627428"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            382..1371
                     /locus_tag="VGT44_15140"
     CDS             382..1371
                     /locus_tag="VGT44_15140"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV2582190.1"
                     /translation="MLQQLIQGFQVTKCLYVAAKLGIADLLKDGPRTSEDLAQVTGTH
                     APSLYRVLRLLTATGLLIEGEAHSFALTPLGTYLQTGVPGSMRTTALNYGGEPTWQVW
                     GDLLHSVKTGEPTYQHVFGCSNWDYRALHPEAAVIFDQTMTEWTASVAPTVAAAYDFS
                     ATQTLVDVGGGHGQLLASILQAHQTLHAVLFDLPHVVKGAPSLLEAAGVADRCKVISG
                     DAFTAVPADYETYLLSRVIHDWDDERAIAILTRCQQAIKPHGKVLLVERVILSGSTPP
                     LLVLESDVNMLVATGGKERTEAEYRTLLSAAGFEVIRMIAVLPPFYLIEAVRR"
     gene            1600..2784
                     /locus_tag="VGT44_15145"
     CDS             1600..2784
                     /locus_tag="VGT44_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEV2582191.1"
                     /translation="MPVPKNKPIIGTPEGSTPDPASPVLPDPGEFRQHLRRLAVSAVQ
                     VLIEQVMLEELEQCIGAAWGECTPTRRGYRNGSYTRDLVTSTGRIEDLKVPRDREGQF
                     HSQVFERYNRYEPEVAEALTQMFVSGVSTHKVGEIAATLTGVAPSASAVSRLNQALTE
                     QYEVWRERRLLPHYRIIYLDGIHFTVRHGERTDATVILTALGVDLEGSREVLALRACA
                     EEDKDGWSCLLQDLRTRGVSQIDLLVTDGHDGLLAAVASLFPATPRQRCLVHKQRNVM
                     NAIPKREQQEVAAELAGIWKQETRESALLNLTGFKAKYQKRYPEAMRSLCEDEEHLLT
                     FYAFPPVMHRYIRSTNAIESFFSNVRSRTDQIDAFTTETSCLTIVWAVTQDIHLPRIP
                     VG"
     gene            complement(3432..4541)
                     /locus_tag="VGT44_15150"
     CDS             complement(3432..4541)
                     /locus_tag="VGT44_15150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2582192.1"
                     /translation="MSVAIRRALPGFTLDVAFDAPPGMTVLFGPSGAGKSLTLQAIAG
                     LLPLDAGSISLDAAVWQDSKRGINLPPQRRHVGYVPQNYALFPHLTVEQNIAFGLKAR
                     GRKGIPGRERVSELVELMQLEGLERRRPAQLSGGQQQRVALARALAADPLLLLLDEPF
                     SSLDAAVREALRDELRALHERVHVPIVLVTHDAAETRLLAETVVVIQQGHVLQVGSPD
                     AVFRSPLTRDVAAMVGMKTCWRGRVVSPPAEGVRVLIGDDLVVYAHVPGGAEVSEGQV
                     LELGIRCDELNVLPGDGDALDEADVSRQATTLVPGMVLRVRPQGAFYSASVLLRTGQA
                     LDIPLSARELRNLDIEAGSRVTVGIPASAVHVFGV"
BASE COUNT         1046 a         1433 c         1364 g          948 t
ORIGIN      
        1 gtgttgtgta ccgaagaggc tggcactcac ggcataaaat ggccgccaaa aacggtctca
       61 tttggccgat aaaccgaagt attcaagcag agtaacgata gtgctaaaac agcttatcct
      121 ggccggaagt gcaaaaatca cccataaggt ggccgaaaat gctttctgta tgatagggtt
      181 tgggagaaag gatagtggtt ggtatctcgg ccactggctc ttgtgcgcaa cctgaaaggg
      241 cgcgaacatg ggtagggtta cgcatgcccg caagcgggtg agggaggtac actcttcacc
      301 agagaatacg ttgccttatg aaaagtggcc acaggccaaa ggaggaacaa catagctacg
      361 aatgatttcc cgccgcagct cgtgctgcaa caactgatcc agggcttcca ggtgaccaag
      421 tgtctctacg ttgcagccaa gctcgggatc gctgacctct tgaaagacgg cccgcgcacg
      481 agtgaagacc tggcgcaggt aacagggacc catgctccct cgctctaccg tgtcctccgc
      541 ctgctgactg ccacaggtct tttgatcgaa ggggaggccc actccttcgc gctcacccct
      601 cttgggacgt atctgcagac cggcgttccc ggctccatgc gcacgaccgc gctcaattac
      661 ggtggagagc cgacctggca ggtctggggg gacttgctcc acagcgtgaa aacgggcgag
      721 ccgacgtatc agcatgtctt cgggtgctcg aattgggact atcgtgcgct ccaccccgag
      781 gcagccgtga tcttcgatca gacgatgacc gaatggaccg ccagcgtggc cccgacggtc
      841 gcggctgcct atgatttctc cgccacacag acgctggtcg atgtgggagg aggacacggg
      901 caactgctgg cgtcgatctt gcaggctcat caaactctgc acgccgtgct ctttgacctg
      961 ccacacgtgg tgaaaggagc gccatccctg ctcgaagcgg cgggagttgc cgaccgctgc
     1021 aaggtcatca gtggagatgc cttcaccgcc gttcccgccg attacgagac gtacctgctc
     1081 tcgcgggtga tccatgattg ggacgacgag cgtgccattg ccattctgac tcgctgccag
     1141 caggccatca agccgcatgg caaagtgttg ctggtggagc gcgtgatcct aagcggcagc
     1201 accccgcccc tgctggtact ggagtccgat gtgaacatgt tggtggcgac aggtgggaaa
     1261 gaacgcaccg aagccgagta ccgcacgctc ctctctgctg cgggctttga agtcatccgg
     1321 atgattgcgg tgttgccgcc gttttacctg attgaggctg tccgtcggtg aggtcatccc
     1381 tctgtgcgga gaggggcagc cttgagaagg ttcgtgatgg cttggagcag gagaggaaag
     1441 agggacaccc gtcacaattc ggtgaggaat ttccccaaaa cggcaattcc tggctgcggc
     1501 caatttatgc cgtgtgtcac agaggctttg aagagaaggc ttgtgagagg atactacagg
     1561 catagatcgt ttccatcttt ccctcgaaag gagaaagcta tgcctgtccc taagaacaag
     1621 cctatcatag gaacccctga ggggtcaacg ccagacccag cgtcccccgt cctgccggac
     1681 ccaggggaat ttcgtcagca tttacgtcgc ctggctgtga gcgcggtgca agtgttgatc
     1741 gaacaagtga tgctcgaaga gctagagcag tgtattggag cagcgtgggg cgaatgcacc
     1801 cccacgcgcc gtggctaccg caatgggtcc tacacccgcg acctggtgac gtccaccggg
     1861 cggattgaag atctcaaagt gcccagggat cgggaaggtc agtttcacag ccaggtgttc
     1921 gagcgctata accggtacga accggaggta gccgaagcct tgacccagat gtttgtcagt
     1981 ggagtgagta cccacaaggt cggagagatc gcggcgaccc tcacgggagt ggcacccagt
     2041 gccagtgcgg tgagtcgcct caatcaggcg ctgaccgaac aatacgaggt ctggcgcgag
     2101 cgtcggctgc tgccccacta tcgcatcatc tatctggatg gcatccattt caccgtgcgc
     2161 catggggaga gaaccgatgc gaccgtgatt ctcacggcat tgggcgtcga tctggaaggc
     2221 agccgcgaag tgttggcctt gcgcgcctgc gccgaggaag ataaagacgg ctggagttgt
     2281 ctcttgcaag acttgcgcac gcgcggcgtg agtcagattg atctgctggt cactgatgga
     2341 catgatggct tactcgccgc ggtagcctcc ctctttcctg ccaccccgcg tcagcgctgc
     2401 ctcgttcaca aacagcgcaa tgtgatgaat gccattccca agcgcgagca gcaagaggtc
     2461 gccgccgaat tggcaggcat ctggaaacag gagacccggg agagtgctct gctcaatctc
     2521 accggcttta aggccaaata tcagaagcgc tatcccgagg cgatgcgcag cctgtgtgaa
     2581 gacgaagaac atctgctgac cttctatgcc ttcccgccag tcatgcatcg ctacattcgc
     2641 agcacgaacg cgattgagag cttctttagc aacgtgagaa gccgaaccga ccagatcgac
     2701 gctttcacga ccgaaaccag ttgcttgacg atcgtctggg cggtgacgca agatatccac
     2761 ttgcccagga ttcccgtcgg ctaacagctg aggcgtgaga ggtttggcaa accaactacc
     2821 agccggtaag gctggttcgg gaggaggtat gtttttgtct tcagcttgtg ctttctcacc
     2881 atccccaggc tctctgttct tcacaacctg ggcatctgcg ctatctcccc ggggctatcg
     2941 ggggatggag agaggcggta tcagcttgac gtctgtactc gcgtgattct gctggtctct
     3001 acacaattct cggcacggtg tctcggagaa caccccgatc actccctggc tatacgtcga
     3061 atcgctcact tgagcgatct gcgtgccatt cacgaacagg gttatattgc tacctcttgc
     3121 tacaactccc agagtattgg tctggcttcc ctgattgatg gctggactga agccactggt
     3181 gagcttattg acagagctgc cattgctata cacctcaagc gtgtaatttc cattcgggta
     3241 gatacggaag caatagaatg agttgccaca ggcgcgaaaa acaacgccgc cagagtaggc
     3301 tagaaacccg ctcgtattcg tttgtatgcg catgttgacg cggaaggcaa agtcactaaa
     3361 gctccgaaat tcagcaaagc agctatattg tgtgttcgtt gtttgagagt acgctgaata
     3421 gtagcacttt ttcatacacc aaacacatgc accgcgctcg ccggtatccc caccgtcacc
     3481 cgcgaaccag cctcaatgtc cagattgcgc aactcgcgag cgcttagagg aatatccagc
     3541 gcctgccccg ttcgcagcag cacgctagcg ctataaaatg ccccttgtgg tctaactcgt
     3601 aggaccatcc ccggcacaag cgtcgtagcc tgccggctaa catcagcctc atctaacgca
     3661 tccccatcgc ctggcaacac attcaattca tcgcaacgaa tacccagctc cagcacttgt
     3721 ccctcagaaa cctccgcacc accaggaaca tgcgcataaa ccaccaggtc atcccctatc
     3781 aacaccctga ctccttcagc cggaggtgag acaaccctcc cccgccagca cgtcttcatc
     3841 cccaccatcg cagccacatc gcgcgtgagc ggcgagcgaa aaacagcatc aggcgagcca
     3901 acctgcagca catgcccctg ctggatcacc acgaccgtct cggccagcaa ccgcgtctcg
     3961 gctgcatcat gcgtcaccag cacaatcggc acgtggacgc gctcgtgtag cgcgcgcagt
     4021 tcgtcgcgca gcgcctcgcg caccgccgcg tcgagcgagc tgaacggctc gtcgagcagc
     4081 agcaacagcg gatcggcggc cagcgcgcgc gccaatgcca cgcgctgctg ctggccgccg
     4141 gagagctgcg cgggccgccg ccgttccagt ccctccagtt gcatcaactc gacaagttcg
     4201 ctcacccgct ccctgccggg tatccccttt ctgccgcgcg ccttgaggcc gaaagcgatg
     4261 ttctgctcaa ccgtgagatg tgggaagagc gcgtagtttt gggggacata ccccacgtgg
     4321 cggcgctgcg gcgggaggtt gatgccacgt ttgctatcct gccacacggc agcatccagg
     4381 gaaatgctgc cagcgtcgag tggcaacaac cccgcgatgg cctgcagcgt cagactcttg
     4441 cccgcgcccg atggcccgaa cagcacggtc atgcccggcg gcgcgtcaaa cgcgacatcc
     4501 agcgtgaaac ccggcagcgc ccggcgaatt gcaaccgaca ataatccagt atgttcctgt
     4561 ccggtaggca aacttgcacc cattatcgag agggaaactt gttctgattg ttccgccccc
     4621 tgactgtcga tcatatcact accgctttcc cggtgcggcg ctgcacgaag acaatcgcgc
     4681 caatcacaag aatgctgagt cccaacgagt acagcgccag gggcagcgcg ccggtgacgc
     4741 ccacgctgga aagctggaca aaggtgaata ccggaatggt atacggatgg t
//
LOCUS       DASZAK010000861         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_234952, whole genome shotgun sequence.
ACCESSION   DASZAK010000861 DASZAK010000000
VERSION     DASZAK010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234952"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="VGT44_15155"
     CDS             complement(<1..1623)
                     /locus_tag="VGT44_15155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582193.1"
                     /translation="MKRSDSGDFYMLYIEPEDDHHSVYDALTAISRQHNKPVVMMLPV
                     TGRARAFARPEDFSDLKRIKRQYELLILFVSASNEHLRHLAARNGFPAYPSIDELSDA
                     LLRGQISFAHQRVMAKTPTPSIPTSRRIEEIDEIRRSPTVGSSARQTVPLTPAAFAKQ
                     SAQPLPFIQPSRLAQSPSTKPSPRATPRAAYASADTPEATTSPNSATEYARPAVKKHR
                     SRGLLVTMIILLMILLGGGGGLGYYFLYAHAATPAPVAAPQSVGQIYFLSSEQVSVTS
                     NQGLNDEVQVDLHNLQPPAPGDSFYAWLLSDVSQGDTTVVYLGRLTVNQGSAHLLYSG
                     DAQHTNLLAVTSRFLVTEESASITPIAPSPDYNTWRYFGIIPQTPNPQDAQHFSLLDH
                     LRHLLASDPLLNELELPGGLCNWFYRNTGKLIEWTVSARDNWQQGRDVAFERRQTLRT
                     LAYLDGLAFIQHDLPANIALPSMPNLASVGLIDVQGANQQPPSYLTHILHHLNGLINA
                     PGATPAQRNAAAQIVTAMSNVQQWFELMRSEAQ"
BASE COUNT          291 a          468 c          548 g          362 t
ORIGIN      
        1 ctgtgcctcg ctgcgcatta gctcaaacca ctgctgcacg ttgctcatag ccgtgacaat
       61 ttgcgcggct gcgttgcgct gcgcgggcgt cgcccctggc gcgttaatca gcccattgag
      121 atgatgcagg atgtgggtca ggtagctcgg tggctgctga ttcgcgccct gtacatcgat
      181 caggccaacg ctggccaggt tcggcatgct cggcagtgcg atgttcgcgg gaaggtcatg
      241 ctggataaag gccagcccat cgagatacgc gagtgtgcgc aaagtttgcc tgcgctcgaa
      301 tgcgacatcc cgtccctgct gccagttgtc gcgcgcgctg accgtccatt cgatcagctt
      361 gccggtgttg cgatagaacc agttgcacag gccgccgggc agttcaagtt cgttcaacag
      421 gggatcggag gccagcaggt ggcgcaggtg gtcgagcagg ctgaaatgct gcgcgtcctg
      481 cgggttgggc gtttgcggga taatgccgaa gtagcgccag gtattgtagt ctggcgaggg
      541 cgcgataggt gtgattgacg cgctctcctc ggtcacaaga aatcggctgg tgactgcgag
      601 caggttcgta tgctgcgcgt ctccgctata aagcaggtgt gcgctcccct ggttgacggt
      661 cagcctgccc aggtacacca cggtggtgtc gccctgcgac acgtcgctca gcagccaggc
      721 gtaaaagctg tctcccggcg cgggcggttg tagattatgc aggtcgacct gtacctcgtc
      781 gtttagaccc tggttgctcg tgacgctcac ttgctcgctg ctcaggaagt agatctgccc
      841 gacgctctgt ggcgcggcca ccggcgctgg cgtggcggca tgggcataca ggaagtagta
      901 acccagccct ccgcctccgc cgagcaggat catcaggaga ataatcattg tcaccagcaa
      961 accgcgcgaa cggtgttttt tcacagctgg cctggcatac tctgtcgctg agttgggcga
     1021 tgtggtcgct tcgggcgtat cggcggaagc gtaggccgcg cgcggtgtcg cacgagggga
     1081 tggtttggtc gatggcgatt gcgcgagccg cgagggttgt atgaatggca atggctgcgc
     1141 ggactgcttc gcgaaggcag caggcgtgag cggaacagtt tgtctggcgc tcgaccctac
     1201 ggtcggagaa cgccttattt cgtcgatctc ctctatccgc cgcgaagtag ggatggatgg
     1261 agtgggggtc ttcgccatga cgcgctggtg cgcgaatgag atttgccccc gcagcagcgc
     1321 gtcgctcagt tcatcgatgg aagggtatgc cgggaaaccg ttgcgcgcgg ctaggtgccg
     1381 tagatgctcg ttactcgcgc tcacaaacag gatcagcagc tcgtattgcc gtttgatgcg
     1441 tttgaggtcg ctgaagtctt ccgggcgcgc gaatgccctg gccctgcccg tcaccggcaa
     1501 catcatcacg actggtttat tgtgctggcg gctgatggcg gtcagcgcgt catacacgct
     1561 gtggtggtcg tcctccggct cgatgtagag catataaaaa tcaccggaat cgctgcgctt
     1621 catgtattcc tccgattacg gcatttcaac caaccaggcg caaccgcga
//
LOCUS       DASZAK010000862         1791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_22220, whole genome shotgun sequence.
ACCESSION   DASZAK010000862 DASZAK010000000
VERSION     DASZAK010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22220"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="VGT44_15160"
     CDS             <1..1186
                     /locus_tag="VGT44_15160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEV2582194.1"
                     /translation="VIVSAVRTPIGRANKGALKDVRADDLAAMAVKAAVERVPQLDRS
                     LIEDVILGCAFPEGEQGMNLARLVGALAGLPETAGGVTVNRFCASSLQAVNMAAQSIM
                     LGLGDVIVAGGIESMSRVPMGGFNPSMNPRLVKQREGADVAMPYPPCELEYGLSSYIP
                     MGMTAENLARDYHISREAQDAFALRSHQRAIAATDNGIFRREIVPVKLPNGSVMEIDE
                     GPRRDTSLEKLASLEPAFIKGGSVTAGNSSPLNDGAAAVVLMSAERARDLGITPLAKV
                     HTMAVAGVRPDIMGIGPVPAMRKVLQRSGMQLSDIDIIELNEAFAAQSLSVVQVMGID
                     EEKLNPHGGAIALGHPLGCSGARIIATLINDLQTYNKTWGIATLCIGGGQGLATLIER
                     LS"
     gene            1189..>1791
                     /locus_tag="VGT44_15165"
     CDS             1189..>1791
                     /locus_tag="VGT44_15165"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2582195.1"
                     /translation="MPEQTYQIRKAAVLGAGVMGAAIAAHLANVGIPSLLLDIVPPGA
                     QDRDIVARKGLSRAMFARPASFYSSRAEKLVTVGNVEDDLEKLGEVDWIIEAVIERLD
                     IKQPLYEKLERVIKPTTIITSNTSGLPAHMLTAGRSATFRRNFLITHFFNPVRYMRLL
                     ELVPDVDTDPALMEFMQHFATETLGKGVVLCKDTPNFIANR"
BASE COUNT          346 a          550 c          582 g          313 t
ORIGIN      
        1 agttatagtc agcgccgtgc gcacccccat tgggcgcgcg aacaagggcg cgcttaaaga
       61 tgtgcgcgcc gatgacctgg cggcaatggc cgtcaaggcg gcggtcgagc gtgtgccaca
      121 actggatcgt tccctgatcg aagatgtcat tctgggctgc gccttccccg aaggggaaca
      181 gggcatgaac ctggcgcggc tcgttggcgc gctggcgggt ctgccggaga ccgccggggg
      241 cgtgaccgtc aaccgttttt gcgcgtcgag cttgcaggcc gtcaatatgg cggcgcagag
      301 catcatgctc ggcctggggg atgtgattgt ggcgggcggc atcgagagta tgtcgcgcgt
      361 gccgatggga ggtttcaatc ccagcatgaa cccgcgcctc gtcaagcagc gcgaaggcgc
      421 ggacgtggcg atgccctatc cgccctgcga gctggagtat ggcctgtcca gttacatccc
      481 gatgggcatg acagcagaga acctggcgcg cgactatcat atcagccgcg aggcgcagga
      541 cgccttcgcg ctgcgcagcc accagcgcgc tatagcagct accgataacg gcatcttcag
      601 gcgcgagatt gtgccggtca aactgcccaa cggcagcgtt atggagatcg acgaggggcc
      661 gcgtcgcgat acctcgttgg agaagctggc gtcgctggaa cccgccttca tcaagggcgg
      721 cagcgtcacg gcgggcaact ccagcccgct caacgatggc gcggcagccg ttgtgctgat
      781 gtcggcggaa cgagcgcgcg acctgggcat cacgcccctg gcgaaggtgc atacgatggc
      841 tgtggcgggc gtgcgccccg acattatggg catcgggccg gttcctgcta tgcgcaaggt
      901 cttgcagcgc tccggaatgc agttgagcga catcgacatc atcgaactga acgaagcctt
      961 cgccgcgcag tcgctttcag tcgtccaggt catgggcatc gacgaggaga agctcaaccc
     1021 gcacggcggc gcaattgccc tggggcatcc gctcggctgc agcggggcgc gcatcatcgc
     1081 cacgctcatc aacgatctgc aaacctataa taagacatgg ggcattgcta cgctctgtat
     1141 cggcggcggc cagggattgg ccacgctgat cgaaaggttg tcgtagccat gcctgagcag
     1201 acataccaga tacgcaaagc cgccgtgctg ggcgcgggcg tgatgggcgc ggccatcgct
     1261 gcccacctcg ccaacgtcgg catccccagc ctgctgctcg acatcgtgcc gccgggggcg
     1321 caggaccgcg acatcgtggc gcgcaaaggt ctcagtagag cgatgtttgc gcgcccggcg
     1381 tccttctaca gctccagggc ggagaagctt gtcaccgtcg gcaacgtcga agacgacctg
     1441 gaaaagctgg gcgaagtgga ttggatcatc gaggcggtaa tcgagcggct cgacatcaag
     1501 cagccgctct acgagaagct cgaaagggta atcaagccca ctaccatcat cacctcgaat
     1561 acctctggcc tgcccgcgca catgctgacg gcgggacgca gtgcgacctt ccggcgcaac
     1621 ttcctcatca cgcacttctt caacccggtg cgttacatgc gcctgctgga actggtgccg
     1681 gatgtagata ccgatccagc attgatggag tttatgcagc acttcgccac ggagacgctg
     1741 ggcaagggcg ttgtgctgtg caaagatacg cccaacttta tcgccaaccg c
//
LOCUS       DASZAK010000863         2933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_305737, whole genome shotgun sequence.
ACCESSION   DASZAK010000863 DASZAK010000000
VERSION     DASZAK010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305737"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VGT44_15170"
     CDS             <1..307
                     /locus_tag="VGT44_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582196.1"
                     /translation="EPLAGAHTNPSLVTQVLRHHLVYAFTELEQVPTKQLLVQRYRKF
                     RHYGQLHHVQHQHAVAVVEQSRHALHQLQYRLPTALSSAWTALVTFGRDRERGITTS"
     gene            complement(502..762)
                     /locus_tag="VGT44_15175"
     CDS             complement(502..762)
                     /locus_tag="VGT44_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582197.1"
                     /translation="MASRILFLCARASSRSLIAASLLAAYEGNRWEVWSTPTQDTLGL
                     HLAERVLREQDIALIPPGSLIQPTFGTRWDEGVILCSGAAET"
     gene            complement(814..1986)
                     /locus_tag="VGT44_15180"
     CDS             complement(814..1986)
                     /locus_tag="VGT44_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582198.1"
                     /translation="MSEYQRYEFMTSDRPLTRAQLDEVDALSSHIDASSTHAVIEYHW
                     GDFKHDPIDVLHRYFDGFLYWANWGSPQLAFRFPHGSLPADLIDGYDLDEFVTLTRHR
                     EFDILDIHFGEMEAPDEWTDYELGSLMPIRDELMEGDQRALYIAWLASRAMIEGYDED
                     DDEDVENGDEDEDEGEESSTPPVPPGFGTLTAAQAALAELLQVPQELLLAAARHSKAA
                     APTSGDDFEAWVKLLPAERHYEYLVRLAHNEPGLSHLFVRELRDLGRDKTRATPPAGE
                     RVPYAILLSESAEISKQLERKKREQEREARLRHLQDIHDHQEDYWRQVDMAAVRASGS
                     GYDEAVRLLVELREVAGHFKESQKFQERFRAWAGNHLRRPALVKRLQAHKFTLPEA"
     gene            complement(2309..2383)
                     /locus_tag="VGT44_15185"
     tRNA            complement(2309..2383)
                     /locus_tag="VGT44_15185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2349..2351),aa:Gly,seq:tcc)
BASE COUNT          642 a          867 c          784 g          640 t
ORIGIN      
        1 ggagcccctg gccggcgcgc ataccaatcc ttccctggtc acacaggtgc tgcgccatca
       61 cctggtgtac gccttcaccg aacttgagca agtccctacc aaacagctgc tcgtccagcg
      121 ctaccgcaaa ttcaggcatt atggacaact tcaccacgta cagcaccaac acgccgtagc
      181 agttgtcgag caaagccgcc acgcccttca tcagcttcaa taccgacttc caacagcact
      241 ttcctcggca tggactgctc ttgtcacctt tggccgagac cgcgaacgcg gcatcaccac
      301 ctcgtagaaa ccgggtcctt atttgtgttg tgcgagtacg ctcagcagcc gcccgtggaa
      361 gcgcaactcc gacgcaatgt gcgcatcgag agcgtcgcgg atgcggcgat agcccgccaa
      421 ttgttgctcc tcgctaagat cgcgtgttgg cgtatcttcg atagaccaca ggcggtgccg
      481 accggcgcca gggatgatcg gtcaggtttc cgccgcgccg ctacacagga tcacgccttc
      541 atcccagcgc gtaccgaagg tgggttgaat gagcgagccg ggtggaatga gcgcgatgtc
      601 ctgttcgcgc aaaacacgct cggccaggtg cagcccaagt gtatcctgcg tgggagtact
      661 ccagacctcc cagcgattgc cctcgtaagc agcaagcagg ctcgcggcaa ttagcgagcg
      721 gctcgatgcg cgagcgcaca gaaagaggat gcgtgatgcc acaatgcctc cttttcagct
      781 aaatccgggt gaggacaacg ctactcaacc atctcacgct tctggcaagg tgaatttgtg
      841 agcctgcaag cgcttgacga gagcgggacg gcgcaggtga ttcccggccc aggcgcggaa
      901 acgctcctga aacttctggc tttccttgaa atgaccggcg acctcacgca gttcgaccaa
      961 caatcgcacg gcctcgtcgt agccgctacc cgaagcgcgc accgctgcca tatcgacctg
     1021 acgccagtaa tcctcctggt gatcgtggat atcttgcagg tgacgcaggc gcgcctcccg
     1081 ctcctgctcg cgcttcttgc gttccagttg cttactaatt tccgcgcttt ccgagagcag
     1141 gatagcgtag ggaacgcgct cgcccgctgg tggtgtcgct ctggtcttat cccggcccag
     1201 atcgcgtaac tccctgacga aaaggtggct cagaccaggc tcgttgtgcg ctaagcggac
     1261 caggtactcg tagtgtcgct ccgctggcag cagcttgacc catgcctcaa aatcatcacc
     1321 gcttgttggt gctgccgcct tactatgtcg agccgccgcg aggagcagtt cttgcggcac
     1381 ctgcagcaat tcggccagcg ccgcctgtgc cgccgtcagc gttccaaaac cgggtggcac
     1441 tggcggtgta ctgctttcct cgccctcgtc ttcatcttca tctccattct caacatcttc
     1501 gtcatcatcc tcgtcgtatc cctcgatcat agcccgactg gccaaccacg caatatacag
     1561 cgcgcgctga tcgccctcca tcaattcatc gcgaatgggc atcagcgatc ccagttcata
     1621 gtcagtccat tcgtcgggcg cttccatctc gccaaagtgg atatcgagga tatcaaactc
     1681 ccggtgccgg gtgagcgtca cgaactcatc gagatcgtag ccatcaatga gatcggctgg
     1741 caagcttcca tgtggaaagc ggaatgccag ttgaggcgaa ccccagttag cccaatacag
     1801 gaagccatcg aaatacctgt ggagtacatc gatggggtca tgcttgaagt caccccagtg
     1861 atactcgata acggcatggg tggaagatgc gtcaatgtga ctggaaaggg catcgacctc
     1921 atcgagctgc gcccgtgtca aaggtcgatc gctcgtcatg aattcgtatc gctggtattc
     1981 gctcatttct gctatcctca agtgtatcta gtgttcatcg tcaaaataac cgcacgactg
     2041 acatagtatg tcgcaaaaaa tatcagagca tatgtcccaa ctacgtaaaa tgctgttcag
     2101 actatactac aagccagtgc aattggcaat acaagaaacc atgtcgatgt tgatagactc
     2161 atctcggctc ccaggaggcc tggagagtaa ggcggcaata tggcgggggc agggtttcgc
     2221 gcacatgcga aggcaggatg tcgctgcaca cttgtattcg ggtgccttct gatcggcctg
     2281 caaagagacg atagaccatg agaccatgtg gagcggaaga cgggattcga acccgcgaca
     2341 gcctgcttgg aaggcaggaa ctctaccact gagttacttc cgctggtaca tcggcttggg
     2401 aggcgacttc aacaccacat cgatggtaca tttgtgtgtg ctgcggccgt ggcataggac
     2461 gttgcgtact cgcctaggca ggatttcacg gcctgttgtt cctcttccgg cactatacta
     2521 tatcccaggg gaaaaggcaa gagcgcgcat gctgtcattg tgtcctctgc tagcaggaag
     2581 gcgatgcccc cgatcttagg aatgtgaccg aaggattgaa tatcagtgtg aatcttcgaa
     2641 cggacatttc tccactttcc ctatgccgtt ccacatcgac taaactctga ggttaaggca
     2701 gtctctcagt ttctaccagc cagaatcgga aatactgaag gtcaactatg attgatgctc
     2761 ttcgagttca tgatttggca catcgacaga acgagctagc tgctcccaaa aggggatgag
     2821 gcttttgctc agttgttttc gcacaccgga cagcctgcgg aagccccctg gcgcttagcc
     2881 ctggtcaccg tgatgcagtt tgcagaagga ctttccgatc gccaggctgc cga
//
LOCUS       DASZAK010000864         1997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_520561, whole genome shotgun sequence.
ACCESSION   DASZAK010000864 DASZAK010000000
VERSION     DASZAK010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520561"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VGT44_15190"
     CDS             complement(<1..292)
                     /locus_tag="VGT44_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582199.1"
                     /translation="MHSTDSTTKAPKTPATVLPVAKAGSASSAPARSRGRKFFSYYKP
                     YLGLLFADMACALIVSAITLLLPLCARYITKNLLDGQTPHALNDIYLIGALML"
     gene            307..471
                     /locus_tag="VGT44_15195"
     CDS             307..471
                     /locus_tag="VGT44_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582200.1"
                     /translation="MIVFKQAQIAQSTNNSARIKHKRAGQSGEDARDREHQHEGNRRG
                     RQDQRGFDGA"
     gene            740..>1997
                     /locus_tag="VGT44_15200"
     CDS             740..>1997
                     /locus_tag="VGT44_15200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEV2582201.1"
                     /translation="MNGFSDKVNFIWSVADLIRDTFKRSKYQDVILPFTVLRRIDCVL
                     QPTKERVLEVNHRLKGRLDNLAPQLRRASGYAFYNTSPYDFERLVSDAPHLADNLRSY
                     INDFSENMREVLEKFEFTNTIAKLDEAGLLFLVMQKFKSIDLHPDRVSNHDMGYIFEE
                     LIRKFNEALNENPGEHFTPREVIRLMVNLLLAQDEAALKREHIVRTVYDPCCGSGGML
                     TIAKERIAELSKHAEVHLYGQELNGETFAICKSDLYMKSADGSDADNIMFGSTLSKDQ
                     HAGKHFDYLLTNPPYGKEWKMDQAAVETEARRGYAGRFGAGTPRISDGQLLFLQHLLA
                     RMQDSDNGGSRVAIVMNGSPLFTGDAGSGESEIRRWILENDWLEAIVALPEQLFYNTG
                     IATYVWVLTNRKEPRRQGKVQLINATD"
BASE COUNT          470 a          583 c          569 g          375 t
ORIGIN      
        1 ccagcatgag cgcgcctatc agatagatgt cgttgagcgc atgaggcgtt tggccatcta
       61 agaggttttt ggtgatgtat cgcgcgcata acggcaggag cagcgtaatc gccgagacaa
      121 tcagcgcgca ggccatgtcc gcgaacaaca ggccgagata tggtttgtag tacgagaaga
      181 acttcctgcc gcgcgaacgc gcaggcgcgc tggatgcaga gccagctttt gccacgggaa
      241 gaacagtcgc cggggtcttc ggtgctttcg tggtcgaatc ggttgaatgc aaagggaata
      301 ctcctcatga tagtgttcaa acaagcgcag atcgcacaga gtacaaacaa ctcggcccgc
      361 atcaaacaca aacgagcggg gcagagtggc gaggatgcgc gcgacaggga acaccagcac
      421 gaggggaatc gacgaggaag gcaagaccaa cgaggctttg acggggccta aatacagctc
      481 catcccttca tgccgggtat cgatgttgcg cggcatggat ttttcctcat tgctccatat
      541 cctttctgaa ctggcggcgt ggggaagcac gcgccaaaga gaattacctg tgtatagtat
      601 gtgatgatga taccatatgg acaatgcctg gtcaagctta aagctgcttg acaattcccc
      661 cgcaatttga taccgttgct ctcgcgatat cctcatctac accgtgttgc tggaagcgct
      721 ggaatagaca ggcgggctta tgaacgggtt tagcgataaa gtcaacttta tctggagcgt
      781 ggccgacctc atccgtgata ccttcaagcg cagcaaatac caggatgtga tcctgccctt
      841 taccgtgctg cgccgcatcg attgcgtttt gcagcccacc aaggaacggg tgctggaggt
      901 caatcaccgg ctcaagggcc ggttggacaa cctcgcgccg cagctgcgca gggcttcggg
      961 ctatgccttc tacaatacct cgccgtatga cttcgagcgg ctggtgagcg acgcgccgca
     1021 cctggcagac aacctgcgtt cgtacatcaa cgatttcagc gaaaatatgc gcgaggtgct
     1081 ggagaaattc gaattcacca ataccatcgc taaactggac gaggccgggc tgctcttcct
     1141 ggtaatgcag aaattcaagt cgatcgacct gcaccccgac cgtgtctcca atcacgacat
     1201 gggttatatc ttcgaggaac tcattcgcaa attcaacgaa gcgctcaacg agaaccccgg
     1261 cgaacacttc acgccgcgcg aggtcattcg cctgatggtc aacctgctgc tggcgcagga
     1321 cgaggccgcc ctgaaacgcg agcatatcgt gcgcaccgtc tatgacccct gctgcggctc
     1381 gggcgggatg ctgaccatcg ccaaagagcg catagcggag ttgagcaagc acgccgaagt
     1441 acacctctat gggcaggagt tgaacggcga gaccttcgcc atctgcaaat ccgacctgta
     1501 catgaagagc gccgacggca gcgacgccga taacatcatg ttcggcagca cattgtcgaa
     1561 ggaccagcac gccggcaagc atttcgacta cctgctcacc aatccgccct atggcaagga
     1621 atggaagatg gaccaggctg ccgtcgagac cgaagccagg cgcggctatg ctggccgttt
     1681 cggggcaggc acaccgcgca tcagcgatgg gcagctgctc ttcctccagc acctgctcgc
     1741 gcgtatgcaa gactccgaca acggcggcag ccgcgtcgcc atcgttatga acggctcgcc
     1801 gctctttacg ggcgacgccg ggagcggcga aagcgagatc cgccgctgga tactcgaaaa
     1861 cgactggctc gaagccatcg tcgccctgcc cgaacagctc ttctacaaca ccggcatcgc
     1921 cacctacgtc tgggtgctga ccaaccgcaa ggagccgcgc cgccagggca aggtacagct
     1981 catcaacgcc accgatt
//
LOCUS       DASZAK010000865         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_554207, whole genome shotgun sequence.
ACCESSION   DASZAK010000865 DASZAK010000000
VERSION     DASZAK010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554207"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            400..1773
                     /locus_tag="VGT44_15205"
     CDS             400..1773
                     /locus_tag="VGT44_15205"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV2582202.1"
                     /translation="MTQLETYITEHENRFLDDLIDWLRIPSISTLPEYAGDIRRAAEY
                     AAEQLRQIGFEQVHLIQTAGHPLVYGEWLKAPGKPTVLVYGHYDVQPVDPVDLWESPP
                     FEPTIRGDNLYCRGACDDKGQTMLVFKALESLMAVNGALPVNVRVLIEGEEEAGGESI
                     DRYVKTYPERLKCDVAFICDTHMLTKEIPTLINGLRGIIYTEVEVHGAKHDLHSGSYG
                     GVAPNPLHALALIIAGLKDAGGHIHIPGLNDKLREASETEKAFWRDDPLHLNEALLEE
                     MGVSEFSGEKEYPPLERIGARPTLEVHGIAGGFIGEGAKTVIPATGKAKISLRLPADL
                     KSDEVYILFERRVKELAPAGVEVTVRKIHGGEAVIVPVDTPPMQAAASALKEVFGREP
                     VFTREGGSIPVAALFYEILNVPVVMMGFGLPDDNLHAPNEKYSLSQFFKGIRTVVRFF
                     QKLGESL"
     gene            complement(1765..>2028)
                     /locus_tag="VGT44_15210"
     CDS             complement(1765..>2028)
                     /locus_tag="VGT44_15210"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEV2582203.1"
                     /translation="PTDIYIHADRYPLLMAFAEALTDYDEVFQLFRTQHFKMAQRMIG
                     PGSIGTGGPPMEMLELTLKDKFYPDLWELRNQLTKIADEEGLK"
BASE COUNT          459 a          503 c          596 g          470 t
ORIGIN      
        1 cttcgtcaac aatagcccac tccaattctc catttggtcc tgccgtgaag ccatcgagtg
       61 agacgtgcat aaatgaaatg acttttctca tggttatact ccttgtttct cgtgtaaacg
      121 tgccgggagt gagcgtgcgg aatgcggcaa cgcccggcct ttgagggcat ggtcagccac
      181 acatgccgtg agcacgaaca gtttaccata cattcgcaga aaaaaacttc tctgatcttg
      241 ctctttggcc gcgacatccg cgaagcttgg tttgaaatgt ggataactat gtggacaaat
      301 gttctaaatt gtggataact tcaagccggt actagacatt caccctttcc cctgctacga
      361 tagagcaaat cagcatgcta aggaaaggac actattttta tgacacaact ggaaacctac
      421 ataacggagc atgaaaaccg ttttcttgat gatctgatag actggctgcg cattcctagc
      481 atcagtacgc tgccggaata cgcgggtgac attcgtcggg ccgcggagta cgctgcggag
      541 cagttgagac aaataggctt cgagcaggtg catttgatac agacagcagg tcacccgctc
      601 gtttatggcg agtggctcaa ggctcccggg aagcccactg tgcttgtata tgggcattat
      661 gatgtgcagc ctgtcgatcc ggttgatttg tgggaatcgc ctccgttcga gccgacgata
      721 cgcggggata atctctattg tcgcggggct tgcgacgaca aaggccagac catgctcgta
      781 ttcaaggccc ttgaatcgct catggcggtc aatggggcgt tacccgtcaa tgtgcgcgta
      841 ttgatcgagg gagaagagga agccgggggc gagtcgattg accgctatgt caagacctac
      901 ccggaacgct taaagtgtga tgtggctttc atttgtgata cgcatatgct aactaaggag
      961 atccccacgc tcatcaacgg cttgcgcggt atcatctaca ccgaggttga ggtgcatggg
     1021 gcaaagcatg acctgcattc aggctcctac ggcggcgttg cgcccaatcc gctgcatgcg
     1081 ctggctttaa tcatcgccgg cctgaaagat gccggggggc atatccacat tccggggctg
     1141 aatgacaagc tgcgcgaggc gtcggagacg gaaaaggcgt tctggcgcga tgatccgttg
     1201 catctgaacg aggcgttgct tgaggagatg ggcgtctcgg agttctcagg cgagaaagag
     1261 tatccacctc tggagcgcat cggggcgcgt ccgactttag aggtgcatgg catcgccggc
     1321 ggcttcattg gtgagggagc taaaacggtg ataccggcga ccgggaaagc caagatcagc
     1381 ctgcgcctcc ctgccgactt gaagtcagat gaggtataca tactcttcga gcggcgtgta
     1441 aaagagcttg ctccggcagg ggtagaggtc acggtgcgca agatacacgg tggcgaggcg
     1501 gtgatagtcc cggtagatac gccgcctatg caggcagcgg cgtctgcgct gaaggaagtg
     1561 ttcgggcgag aacctgtttt cacccgtgag ggaggttcga tccctgtagc ggcactgttt
     1621 tatgaaatcc tgaatgtgcc ggtggtgatg atgggttttg gtttgccaga cgacaacctg
     1681 cacgcaccga atgagaaata ttcactttcc cagttcttta agggcattcg cactgttgtg
     1741 cgcttcttcc aaaaattggg agagtcactt taagccctct tcatcagcga tcttcgtcaa
     1801 ctggttgcgc agctcccaca ggtccgggta aaacttatct ttgagggtaa gctcgagcat
     1861 ctccatgggc ggacccccgg tgccaatgga ccctgggccg atcatacgct gcgccatctt
     1921 gaaatgctgg gtacggaaaa gttggaatac ctcgtcatag tcggtcagcg cttcggcaaa
     1981 ggccatcagc aggggatagc ggtcggcgtg gatgtagatg tccgtggg
//
LOCUS       DASZAK010000866         1764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_22364, whole genome shotgun sequence.
ACCESSION   DASZAK010000866 DASZAK010000000
VERSION     DASZAK010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22364"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1144)
                     /locus_tag="VGT44_15215"
     CDS             complement(44..1144)
                     /locus_tag="VGT44_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2582204.1"
                     /translation="MAILNPEREAYSALLGSDAPALPTTKGLDTGVLYDNPFTRRETE
                     PLIAVQRHVDDAADDVTLDELEESSESELLEITPRIRRSRDTNARHASGAEDAFQSYL
                     RDIRGLGLLTYEEEVTLARRAAAGDAYARRTLIESNLRLVIAIARRYTSTGVPLIDLI
                     QEGNLGLMRAAEKFDYQRGCHFGTYATWWIRQAVSRAAGEQSRLIHLPEHVATRLRKV
                     RRIAAQLSQENGLDPLPEQIAEACNLDLGEVVDLLGIIEQPVSLDAPVDDEARYSLSD
                     TLEDSAAPAPAETASQHLLGEELHRALAALTPRERSVITLRYGIGDGRSRTLLEVGKE
                     LGISRERVRQLEVVALMKLRGITNGVRLQECV"
     gene            1501..>1764
                     /locus_tag="VGT44_15220"
     CDS             1501..>1764
                     /locus_tag="VGT44_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEV2582205.1"
                     /translation="MDQVYIHPTAEVSPDAIIGPGTSLWNQAQVRAGANIGASCNIGK
                     NVYIDAHVVIGNKVKIQNNVSIFHGVTLEDGVFIGPHVCFTNDV"
BASE COUNT          363 a          473 c          506 g          422 t
ORIGIN      
        1 gcctcgccgc tccaccgccc cctgtcccta cactttgatt ggcctacacg cattcttgca
       61 gacggacacc attggtgatc ccgcgcagtt tcatgagcgc caccacttcg agctggcgca
      121 cgcgctcgcg ggagataccg agttctttgc cgacttcgag cagggtgcgg ctgcgaccat
      181 cgccgatgcc gtagcgcaga gtgatgacag agcgctcgcg aggagtcagc gcagccagcg
      241 cccggtgcag ttcttcaccg agcaagtgct gcgaggcggt ttcggcgggt gcaggcgctg
      301 cgctatcttc gagcgtatcc gagagcgaat agcgcgcttc gtcgtcaacg ggtgcgtcaa
      361 gcgagacggg ttgctcgatg atgcccagca gatctacgac ttcgccaaga tcgagattac
      421 acgcttctgc aatttgctct ggcaggggat cgaggccgtt ttcctgggag agttgggctg
      481 cgatacggcg gactttgcgc aggcgggtcg cgacgtgctc gggcaaatgg atcaagcgag
      541 attgctcacc ggcagcccgg ctgacggcct ggcgtatcca ccaggtggca taggtaccaa
      601 agtgacagcc gcgctggtaa tcgaactttt cggcggcgcg catcagcccc aggttgccct
      661 cctggatcag gtcgatcaag ggaaccccgg tgctagtata gcgacgcgca atagcgatca
      721 ccaggcgcag gttcgattca atcagggtgc gtcgcgcgta ggcgtcaccg gcggcggcgc
      781 ggcgcgccag ggtgacttct tcttcgtagg tcaggaggcc gaggccgcga atatcgcgca
      841 ggtagctttg aaaggcatcc tcagctccgc tagcatggcg cgcgttggtg tcgcgactgc
      901 ggcggatgcg tggcgttatt tcgagaagtt cgctctcgct ggactcttcc agttcgtcca
      961 gggtaacgtc atccgcggca tcgtcaacgt gccgttgcac tgcaataagc ggttcagttt
     1021 cgcgcctcgt aaaaggatta tcgtacaaaa ctccggtgtc aaggcctttg gtagtgggaa
     1081 gcgccggtgc atcagagcct aacagcgcgc tgtatgcttc ccgctcaggg ttcaaaatgg
     1141 ccattttcca cgctcctttg gggaactccc ccgacaaaaa cagctgccgg actcctccgt
     1201 ttggctccgt ggttagcaca tgtctttgcc tatgtgctga cttagtttgc tatttctatt
     1261 gtatttgaag caaatggcat atcacatcag gtaattacct tagatcaaat caggttctga
     1321 ctgtatattg ccattcttca caggattcac tatgtattac gacgtatgat ccatttcagt
     1381 ttctaaaatc gtatcggtca aattcaattg aacttgctga tacactgaga agtaggtata
     1441 gcttgtctat cctgatgtat ccacgctggg taattggtgt tgttgaaatg gaggtgtatc
     1501 gtggatcagg tttatattca tccgactgca gaggtatccc ccgatgccat aatcggccca
     1561 ggcacatcac tttggaatca ggcgcaggtg cgtgcgggtg cgaatattgg cgcatcctgc
     1621 aacatcggca aaaatgtgta tattgatgcc cacgtcgtaa ttggcaacaa ggtcaaaatt
     1681 caaaacaacg tttcgatctt tcacggtgtc acgttggaag atggcgtctt catcggcccg
     1741 catgtctgtt tcacaaatga tgtg
//
LOCUS       DASZAK010000867         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_483647, whole genome shotgun sequence.
ACCESSION   DASZAK010000867 DASZAK010000000
VERSION     DASZAK010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483647"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1068
                     /locus_tag="VGT44_15225"
     CDS             160..1068
                     /locus_tag="VGT44_15225"
                     /inference="COORDINATES: protein motif:HMM:NF014764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582206.1"
                     /translation="MEQSKALIWNPILRQYLVNAPNRMNRREGTNACPFCEDIIQGKV
                     GPQTQVWLHPNDFPPFTPPTGEAYVVIYNRDHHRSFVQLSVDEVSAVTHLWRDLYRDL
                     ASRYPAIMIFENSGEAIGQTQHHPHGQAFGVSVIPPTLEREMETVVMDDAAGRGCPFC
                     RVRADLTDSEYQVAMNASWQAFLPPYVRYPYETHLYPRRHIAHIGGMQDDELRDLAAL
                     LLQVTRGYNALYDGAMAPMPYLLGLHQLADERFHLHVEILVIGRAPGKLKFAASSESI
                     WGLWTNDSSPAQKAQELREAIAKEQR"
     gene            1065..2345
                     /gene="galK"
                     /locus_tag="VGT44_15230"
     CDS             1065..2345
                     /gene="galK"
                     /locus_tag="VGT44_15230"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV2582207.1"
                     /translation="MMMQNPMLPRPARDALEEFPLVFQDEAWPAEQIGVAWAPGRVNL
                     IGEHTDYNDGYVLPLAVDRVVAFAGRPRADSLVRLWSGYFSEEAQLDLSDLPASFDEQ
                     LQRLPMWARYVLGVMTELKRAGVALRGFDALIEGDVPVGGGMSSSAAIEVATAHACML
                     FSDGNFTIGAAGATMQPLQVAALCQRAEHIASGVRSGILDQAASCLGQAGKAVLLDCR
                     SLDYRYLPFDQPGIALVVIDTGVRRDLAASAYNERRAQCEEATRLLREMILRDEPREA
                     SATNIRALRDITPEQLARYADRLPDVLRRRAAYVIAENARVLETALLLERHAIEQVGP
                     LLWKSHEGLRDDYEVSCMELDTLVEIAREVPGVLGARMLGGGFGGCTINLVRYAALEQ
                     LHQAVETNYMARTGRQASIDVCRAAGGPGSVWAT"
     gene            complement(2373..2576)
                     /locus_tag="VGT44_15235"
     CDS             complement(2373..2576)
                     /locus_tag="VGT44_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582208.1"
                     /translation="MSYSIEIHIDVEELQLPGDLTALEQIEAAIEGMVVDVLEPWVST
                     MAVREVMVNLISQAERDWDALLK"
BASE COUNT          578 a          858 c          922 g          606 t
ORIGIN      
        1 ctgagcatcc ctgaaatggc aaaacgtctt tgctagaatt atagcatgaa aagtgaggca
       61 tattgtatac tgggatgcgc gctacatttt tgcgtcgaga gtggctgaaa gggcgaccac
      121 cagggtacgt cctgttacaa aggtcaggta gtataaagta tggagcaatc caaagcccta
      181 atctggaatc ctattttacg ccagtacctg gtcaacgcgc ccaatcgcat gaaccgtcgc
      241 gaaggaacga acgcctgccc gttttgtgaa gatatcatcc aggggaaggt cggtccgcag
      301 acgcaggtct ggctgcatcc gaacgatttc ccacccttca cgccgccgac aggggaagcc
      361 tatgttgtaa tctacaaccg cgaccaccat cgctcgtttg tccagctttc ggtggacgag
      421 gtgagcgcgg tgacgcatct gtggcgtgac ctctaccgcg acctggcctc gcgctatcct
      481 gctatcatga tctttgagaa cagtggggag gcgattggac agacacagca tcacccgcac
      541 ggccaggcgt tcggcgtctc ggtgatcccg ccaacgttag agcgcgaaat ggagacggta
      601 gtcatggacg acgctgccgg ccgcggctgc cccttctgcc gtgtgcgcgc ggacctgacc
      661 gacagcgagt accaggtagc gatgaatgcg agctggcagg cgttcctgcc gccctatgtg
      721 cgctaccctt acgagacgca tctctaccca cgccgccaca ttgcccatat cggcgggatg
      781 caggatgatg aactgcgcga cctggccgcg ctgttactcc aggtgacgcg cggctataac
      841 gcgctctatg atggcgcgat ggcccccatg ccgtatctgc ttggtctgca ccagcttgct
      901 gacgagcgtt ttcacctgca cgtcgaaatc ctggtgatcg gtcgcgcgcc cggtaagctt
      961 aagtttgccg cctcatctga atcaatctgg ggactctgga caaacgactc ttccccggcg
     1021 cagaaggcgc aggaactgcg tgaagcgatt gcgaaggagc agcgatgatg atgcagaacc
     1081 ccatgctgcc gcgaccggcc agagatgcac tggaggagtt cccgctggta tttcaggatg
     1141 aggcgtggcc cgcggaacaa ataggcgtgg cctgggcgcc gggacgtgtc aacctgatcg
     1201 gagaacacac cgactataac gatgggtatg tgctgccgct tgcggtagac cgcgtggtgg
     1261 cctttgccgg gcgaccgcgt gcggatagcc tcgtgcgact ctggtcaggc tatttcagcg
     1321 aagaggcgca gcttgacctc tctgacctgc ccgcgtcctt cgatgaacag ctccagcgtt
     1381 tgccgatgtg ggcgcgctat gtgctgggcg tgatgaccga gttgaagcgc gcgggtgtgg
     1441 cgttgcgcgg cttcgatgcg ctgatcgaag gcgatgtgcc ggtgggcggc ggcatgtcct
     1501 cgtcggcggc tatcgaggtg gcgactgccc atgcatgtat gctcttctcc gatggcaact
     1561 tcaccattgg cgcggcgggg gcgaccatgc agccgctgca ggtggcggcg ctctgccagc
     1621 gcgccgagca tatcgcatcc ggcgtgagaa gcggcatcct tgaccaggcg gcctcgtgcc
     1681 tggggcaggc tggcaaggca gtgctgctcg attgccgctc gcttgactat cgttatctcc
     1741 ccttcgatca gccaggtatc gcgctggtgg tgatcgatac cggcgtgcgg cgcgatctgg
     1801 cggcatcggc ctacaacgag cggcgcgcgc agtgcgagga ggccacccgg ctgttacgag
     1861 agatgatctt gcgcgatgag ccgcgtgaag catcggcaac caacattcgc gcattgcggg
     1921 atattacgcc ggagcaactc gcgcgctacg cggatcgcct gccggatgtg ctgagacggc
     1981 gcgccgcgta cgtgatcgcg gaaaatgcgc gcgttctgga gacggcgctt ctgctcgaac
     2041 gtcatgccat cgagcaagtg gggccgctct tgtggaaatc gcacgaaggg ctgcgtgacg
     2101 attacgaggt cagttgtatg gagctagata cgctggttga gattgcgcga gaggtgccgg
     2161 gtgtgctggg cgcgcgaatg ctcggcggtg gctttggcgg ttgcacgatc aatcttgtac
     2221 gctatgcggc tctcgaacaa ttgcaccagg ccgtcgaaac gaactatatg gcccgcacag
     2281 gccgccaggc cagtatcgat gtgtgccggg cggcgggcgg gccgggcagc gtgtgggcta
     2341 cctgatcgca gcgcgctgcc acgccacacc tctcacttta gcagggcatc ccaatctctc
     2401 tcagcctggc tgatgaggtt aaccatgacc tcccggacgg ccatggttga aacccatggt
     2461 tccagtacat ccacaaccat gccctcaatc gctgcttcaa tctgttcaag cgctgtcaga
     2521 tcgcctggaa gctgaagctc ttcgacatca atgtgtattt cgatagagta cgacatgccg
     2581 ctcactcctt tcgcctgtgc gttcgctcat ctctgtgggt ctcctcccct ataaacacag
     2641 cttatcaggt agcggcagga aagaggatat caaacttatc aaatacatat cattttttca
     2701 tcaaaggagc gaaatgtaga agcagagcag ttaataacgc gcctgggggc ggcgcgctaa
     2761 tggagcatgg aagacgagga cgaccgcaag gatctccacc cttgcggtcg ccctggggct
     2821 tgcagggtta catggcctca gccgggaaat gttcctgcgt cacttcttcg tcgacgctga
     2881 gccgcctgcc gggagcgcgg tccgtgcgat caacgcggtc tagcatctcg gtcagcccca
     2941 gcaggcgctg ctcaagttcc catg
//
LOCUS       DASZAK010000868         2190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_697351, whole genome shotgun sequence.
ACCESSION   DASZAK010000868 DASZAK010000000
VERSION     DASZAK010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697351"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="VGT44_15240"
     CDS             <1..1677
                     /locus_tag="VGT44_15240"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEV2582209.1"
                     /translation="LNINVLLEQFHRYAASIVSVLIVATTVLAIIYARKQRQVLIPAL
                     IAPAFLVVQIVLGALTVLWKLPPQIITAHLGTALVIFAMVITVAIMAGKAKTSKESPT
                     KTRKFARLAITNAILVYGLLLSGSYVTNSNAELACPGWPICSNPPQWAVQLGLADINV
                     FHRLVATFVGLVMLWTIISALRRRKVAPGQALVALCGGVLFLAQATVGGLVVLLNGPD
                     FVDALHLALATAVWGTMVALAVLAARQLRASPEGQELEEIEAREAQEQKEVGPIRQTI
                     SGYIDLMKPHVTVLLLGTTVAAMAIAKQGLPPLGLVFATLLGGAMSAGSANCINCYID
                     RDIDQIMGRTQRRSLPSGRVQPTQALIFGLALGFGSFAILAIFVNMLSALLATSAILF
                     YVFVYTMWLKRSSAQNIVIGGAAGAVPVLVGWAAVTNTITLPAIWLFAIIFYWTPPHF
                     WALSLLIQKDYEKASIPMLPVVMGERETRKQIFLYSLLLLAVTMILFAMQAMGLFYLI
                     CALVLGGILVYMAVRLLRDATKKWARTLFWYSNCYLALIFAVMVLDRVVH"
     gene            1687..>2190
                     /locus_tag="VGT44_15245"
     CDS             1687..>2190
                     /locus_tag="VGT44_15245"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEV2582210.1"
                     /translation="MAMSWRLASRLSVVILALLVILVVALFSLRDSFGSPGGLGQSGV
                     SSGLQGTELDGITAPDFRLKDQFGREVSLSQFRGKPVVVTFLYTHCPGVCPLIADQLH
                     ATALGLGKDVGRVAFLAVSVDPQHDTPASAIHFSNVHKMTRYWHYLMGTRDQLTPVWS
                     AYGIDEQP"
BASE COUNT          379 a          593 c          699 g          519 t
ORIGIN      
        1 ctgaatatta atgtgctgct ggagcagttc catcgctacg cagcttcgat tgtcagcgtg
       61 ttgattgtgg ccacgacggt gctggcaatc atttatgcga ggaagcagag gcaggtgttg
      121 attcctgccc tgattgcgcc agccttcctg gtagtgcaga tcgtgctggg cgcgctgacg
      181 gtgctgtgga agctgccgcc gcagatcatc acggcgcacc tgggtacggc gctggtcatc
      241 ttcgcgatgg tgattacagt ggcgatcatg gcgggcaagg cgaagacgag caaggagtcg
      301 ccgacgaaga cgcgcaagtt cgcgcggctg gcgattacga atgcgatcct ggtatatggg
      361 ctgctcttga gcggctcgta tgtgacgaat agcaacgcgg aactggcctg ccctggctgg
      421 ccgatctgta gcaatcctcc acagtgggcg gtgcagttgg ggctggcgga tatcaacgtc
      481 tttcaccgcc tggtcgccac gtttgtcggg ctggtgatgc tctggacgat tatttctgcc
      541 ctgcgcaggc gcaaggtcgc gccggggcag gcgctggtag cgctgtgcgg aggtgtgctg
      601 ttcctcgcac aggcgacggt tggtggcctg gttgtgctgc tgaacggccc tgactttgtg
      661 gatgcgctgc acctggcgct ggcaactgcc gtatggggga cgatggtcgc gctggcggtg
      721 ctggccgctc gccagctgcg cgcgtcaccg gaaggacagg agcttgagga gatcgaagcc
      781 agggaagcgc aagaacagaa ggaagttggc cccatccggc agaccatctc cggctatatc
      841 gacctgatga agccgcatgt gacggtgctg ctgctcggca cgaccgtcgc ggcgatggcg
      901 attgcaaagc agggactgcc gccgctcggc ctagtcttcg cgacgctgct gggcggcgcg
      961 atgtcggcgg gaagcgccaa ctgcatcaac tgctacatcg accgcgacat cgaccagatc
     1021 atgggacgca cccagcgtcg ctcgctgccc tccggcaggg tacagccgac ccaggcgctg
     1081 atcttcggcc tcgcgttggg gttcggttcg ttcgcgatcc tggccatttt tgtgaatatg
     1141 ttgagcgcgc tgctggccac ctcggccatc ctgttctatg tgttcgtgta tacgatgtgg
     1201 ttgaaacgct cctcggcgca aaatatcgtg attggcggcg cggctggcgc ggtgcctgtg
     1261 ctggtgggtt gggcagccgt gacgaacacg attacgctgc cggccatctg gctcttcgct
     1321 atcatctttt actggactcc gccgcatttc tgggcgctct ccttactcat ccagaaagat
     1381 tatgaaaagg ctagcattcc aatgctgccg gttgtgatgg gtgaacgaga gacgcgcaaa
     1441 caaatcttcc tctattcgct gctcctgctg gcggtgacga tgatcctgtt cgcgatgcaa
     1501 gcgatgggtc ttttctacct gatttgcgcg cttgtgctgg gtggtatact ggtatacatg
     1561 gcggtacgcc tgctgcgtga tgcgacaaaa aaatgggcgc gtaccctctt ctggtactcg
     1621 aattgctatc tcgcgctgat ctttgccgtc atggtactcg acagggtcgt gcattaaggt
     1681 tcgtttatgg ccatgagctg gcgcctggct tctcgtcttt cggtggtaat actggcgtta
     1741 ctggttatac tggtggttgc actattcagc ctgcgtgata gcttcggctc gcctggcggt
     1801 ttaggacaat caggggtgtc gtctggcttg caagggacgg aactggatgg catcacagcg
     1861 ccggactttc gcttgaaaga ccagtttggc agagaggttt ctctttcgca gttcagaggg
     1921 aagccggtcg tggtgacgtt cctttatacg cattgcccag gtgtttgccc gcttattgcc
     1981 gatcaactgc acgcgaccgc gctcggcctg ggcaaggatg ttggccgagt cgctttcctg
     2041 gccgtcagcg tagaccctca acatgataca ccggcttccg cgatacattt ctccaatgtg
     2101 cataagatga cgcgctactg gcattacttg atgggtacgc gcgaccagtt gacccccgtc
     2161 tggtcggcat acggtatcga tgagcaacct
//
LOCUS       DASZAK010000869         4396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_22457, whole genome shotgun sequence.
ACCESSION   DASZAK010000869 DASZAK010000000
VERSION     DASZAK010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22457"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1329)
                     /locus_tag="VGT44_15250"
     CDS             complement(64..1329)
                     /locus_tag="VGT44_15250"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEV2582211.1"
                     /translation="MPEQNGMIFHTHRLSNGLQIVAQPMPDFESVAVSYYVNTGSRDE
                     PDPSIAGVSPFLEQMVFKGTQTLDWQQITLEFNKIGAELNAYTSHEATVFFARVLGEY
                     LERALALLSDMMYPRLDESDFTMEKEVIINEIARSEDQPYSLAYRRMMQAYFGDHPLG
                     HDVLGSRESIRNLRIEQMRDYWKRRYAANNLILAVAGNFDWNHLLELAEKHAASWRVG
                     DAGRSTEMYEPAHSHNEVMIDPKLKQQIMFLSMPCVDVKDPDYYAAMLGGSILGDGDG
                     SRLYWNIYQKGLAESASAGIWAMEGTGILMMEANSTPEQAPRVLKMLRAELDSLLDDG
                     VHEDELRRAKDKIISHMVLSSESTFARMRALSADWITEGRLLTVDEEVQRVENVSTTD
                     VLRALRRFPMRDKQVLTTLGPLSEAELLN"
     gene            complement(1446..2759)
                     /locus_tag="VGT44_15255"
     CDS             complement(1446..2759)
                     /locus_tag="VGT44_15255"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2582212.1"
                     /translation="MSLMQSETNKIATRRPTTATQPRRYRRARGLPAIFQSAGSALQA
                     LRANLLRSLLTSLGIIIGVGAVIMMIAISEGNTSTINARLSTLNPLQLTIRPGSATAT
                     GVRQGAGTQQTLTQADADALAQLSNVAAVSPVLNVSGQVVFGNQNWQTTTQGVYPDYQ
                     QIGSWQVQEGNFFTQSDEQGDTNVAVIGQTVVDNLFTPLGIDPVGQQIRIGSVSFTVV
                     GTLVAKGSTGFGANADDVVYVPFSTAQQRLSGSTFVNSIVMTANNASTVNSVQAAAQQ
                     LLEQRHNITNPANDDFTIQNVAQTLSTVQATSSALTTLLVGVAAISLVVGGIGIMNIM
                     LVSVTERTREIGIRIAVGARQRDVLTQFLIEALTLSTLGGIVGLLIGIAGSFIVSSVN
                     GFPFVLDPLSLLLAVGVSALVGVIFGFYPAQRAARLDPIVALRTE"
     gene            complement(2756..3535)
                     /locus_tag="VGT44_15260"
     CDS             complement(2756..3535)
                     /locus_tag="VGT44_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2582213.1"
                     /translation="MQNEQNTAPPVIQVRRLTKNYYLGRTVVPALRCVDLAIARGEFI
                     ALMGPSGSGKTTFMNLLGCLDRPSGGIYLLDGVPVSRLNTNQLADVRNAKIGFVFQSF
                     NLLSWMNALDNVQLPLVYAGVPKEERHKKATWALSMVGLRSRARHRPAELSGGQQQRV
                     AIARALVTSPSLFLADEPTGNLDSQTSTQIISILQELNARGLTVVLVTHEPDIASYAQ
                     RQVKFRDGRIVGDSQSFILQDTQPLPLLTASKSTEDAQVTP"
     gene            complement(3541..>4396)
                     /locus_tag="VGT44_15265"
     CDS             complement(3541..>4396)
                     /locus_tag="VGT44_15265"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV2582214.1"
                     /translation="ASQSQLDQDNNSLQSAKLALKTAQDNLAATTMTAPGNATVAAVN
                     GIVGQNVTSGGSSASSSSSTSFIVLVDLSSFTISSQVNEADIGKVQVGQPVQFTVNAY
                     PSATFRATVSSVDIIGQTSSSVVSYPVVMSVDMNSLNGAHLYPGMTATANITTAQRIG
                     ATLVSNSAFSFITTAEQAGVINRNTLLSALRGSQGTGTGAGNQRLVLVMKNGQLTPIL
                     VTVGLTNGSFSEVLAGLQPGEQVVVGATGGPFANLSSSSSNSNSAGLLRGLGGSGGGA
                     GKGKNSGG"
BASE COUNT          809 a         1255 c         1421 g          911 t
ORIGIN      
        1 atgtaaggca tctacggtca aatatttagg taacaacccc cgcatgcctg ggggagtgtg
       61 tcattagttc aatagttcgg cttcgcttag cggacccagg gtggtcagga cttgtttgtc
      121 gcgcataggg aatcggcgca gggcgcgcag tacgtcggtt gtgctcacgt tttcgacgcg
      181 ctggacctct tcatcaacgg tgaggaggcg gccttcggtg atccagtcgg cggaaagggc
      241 gcgcattcgg gcaaaggtgg actcactgct gagaaccatg tggctgatga ttttgtcttt
      301 ggcgcggcgc agttcgtctt cgtggacgcc gtcgtcgagc aggctgtcga gttcggcgcg
      361 cagcatcttc agtacgcgcg gcgcttgctc cggcgttgag ttggcctcca tcatcagaat
      421 gccggtgcct tccatagccc agatgcccgc gctggccgac tccgccaatc ccttctggta
      481 gatgttccag tagaggcgcg acccgtcgcc gtcgcccagg atgctgccgc ccagcatggc
      541 tgcgtagtaa tcggggtcct ttacatccac gcacggcatg gaaaggaaca taatctgctg
      601 cttgagcttc ggatcgatca tgacctcgtt atgtgaatgc gccggttcat acatctcagt
      661 gctgcgcccg gcatcgccga cacgccagct agccgcgtgc ttttcggcga gttccaggag
      721 atgattccaa tcgaaattgc ccgcgacggc caggatcagg ttattggcgg cgtagcggcg
      781 cttccagtaa tcgcgcatct gctcgatacg caggttgcga atgctctcac gcgagccaag
      841 cacatcgtgg ccgaggggat gatcgccgaa atacgcctgc atcatgcgcc ggtaggccag
      901 gctgtagggc tgatcttccg agcgcgcgat ttcattgatg atgacctctt tctccatcgt
      961 gaaatcgctc tcatccaggc gggggtacat catatcgctg agcagcgcca gggcgcgctc
     1021 gaggtattcg cccaacacgc gcgcgaaaaa gacggttgcc tcgtgcgagg tgtaggcgtt
     1081 gagttccgcg ccaattttgt tgaattcgag cgtgatctgc tgccagtcca gcgtctgcgt
     1141 tcccttaaag accatctgct ccaggaaagg cgagacgccc gcgatggatg ggtctggctc
     1201 gtcgcgcgag ccggtattga catagtagga cacggccacc gactcaaaat cgggcatggg
     1261 ctgcgccacg atttgaaggc cattcgacag gcgatgtgta tgaaagatca ttccgttctg
     1321 ttccggcatg agtaagctcc ttaagattat ctgagggcga ccgcaagggt gtaccctttg
     1381 ggacccaccc actattacat agtagcatga tagccctgcc gggggagcaa tgtcaaatct
     1441 gaagtttact ccgttctcag ggcgacgatg gggtccaggc gggcggcacg ctgggccggg
     1501 tagaagccga agatgacgcc gaccagggcg gagacgccga cggctagcag cagcgagaga
     1561 gggtcaagca caaacgggaa gccgttgacg ctggacacga tgaacgaacc tgcaatgccg
     1621 atcagcaagc cgacgatgcc acccagcgtg ctgagcgtca gggcctcgat caggaattgc
     1681 gtcagcacat cgcgctggcg cgcgccgacg gcgatgcgaa tgccgatctc gcgtgtgcgt
     1741 tcggtgacgg agaccagcat gatattcata atgccgatgc cgcctactac cagcgagatg
     1801 gccgctacac cgaccagcag cgtcgtgagc gccgaggatg tcgcctgtac ggttgagagc
     1861 gtctgggcga cgttctgaat agtgaagtcg tcattggcag gattcgtgat attgtggcgc
     1921 tgctccagca actgctgcgc tgctgcctgc acgctgttga ctgtgctggc gttattagcg
     1981 gtcataacga tgctgttgac gaatgtgctg ccgctcaaac gctgctgcgc ggtgctgaac
     2041 gggacgtaga ccacgtcatc agcgttggct ccgaagccgg tcgaaccttt ggcgaccagg
     2101 gtgccgacga ccgtgaagct gacgctgccg atgcgtatct gctgccccac cgggtcaatg
     2161 ccgagcggtg tgaagaggtt atcgacgacc gtctgcccga tcaccgcgac gttcgtatcc
     2221 ccctgctcgt cggattgtgt gaagaagttg ccttcctgca cctgccagct gccgatttgc
     2281 tggtagtccg ggtagacgcc ctgggtggtg gtttgccagt tctggttgcc gaagacgact
     2341 tgcccgctga cgttgagaac aggactgacg gctgccacat tcgacaactg cgccagggca
     2401 tccgcgtcag cctgggtgag ggtttgctgc gtgccggcac cctgccgcac accggtagca
     2461 gtggcgctgc cggggcgaat ggtgagctgg agagggttca gcgttgacag gcgcgcgttg
     2521 atggtggagg tgttgccttc gctgatggcg atcatcataa tcaccgcgcc cacgccgatg
     2581 atgatgccca acgatgtgag cagcgagcgc agcaggttgg cccgcagcgc ctgcagcgcg
     2641 ctgcccgcgc tctggaagat ggcgggcaac ccgcgcgcgc ggcggtagcg tcgcggttgg
     2701 gtcgcggtag ttggccgccg tgtcgcgatc ttgttcgttt cggattgcat taaactcatg
     2761 gtgtcacctg tgcatcttcg gttgatttag aggcggtgag cagcggtaga ggctgcgtgt
     2821 cctggaggat gaagctctgc gaatcgccta caatgcgccc gtcgcggaac ttgacctgcc
     2881 gttgagcgta gctggcgatg tctggctcgt gggtgacgag gacgaccgtc agcccgcgcg
     2941 cattcagttc ctgaagaatc gagatgatct gagtgctggt ctggctatcc aggttgcctg
     3001 tcggctcatc cgccaggaag agcgaggggg atgtgaccag ggcacgcgcg atggcgacgc
     3061 gctgctgctg gccgcctgac agctccgccg ggcgatggcg cgcgcgcgaa cgcagcccga
     3121 ccatcgataa ggcccacgtc gccttcttgt gccgctcctc tttgggtacg cctgcgtaca
     3181 ccaggggcaa ttgaacgtta tcgagcgcgt tcatccacga gagcaggtta aagctctgga
     3241 agacgaagcc gattttcgcg ttgcgcacat ccgccagctg gttcgtattc aaacggctaa
     3301 ccggcacgcc atcgagcagg tagattccgc cggaagggcg gtccaggcag cccagcaggt
     3361 tcatgaaagt ggtcttgccc gaaccggatg gccccatcaa ggctatgaac tcgccccgcg
     3421 cgatcgcgag atcgacgcag cgcagcgcgg gtacaacggt acgccccagg tagtagtttt
     3481 ttgtcaggcg ccgcacctgg atgacgggcg gcgccgtgtt ttgttcgttc tgcatgagac
     3541 ctagcctcct gagttctttc ccttaccagc gccgccgcca ctgccgccca ggccgcgcag
     3601 cagacccgcg ctattcgagt tggagctgga actcgacagg ttcgcgaacg gtcccccggt
     3661 cgcgcccact accacctgct cgccgggctg caggcccgcc agcacttcgc taaaggagcc
     3721 gttggtcagg ccgaccgtca ccaagatggg ggtgagctgc ccgtttttca tgacaagcac
     3781 gagacgctgg ttgccggcgc ctgttcctgt tccctgcgaa cctctgagag cagaaagcag
     3841 cgtatttcga ttgattacgc cggcttgctc tgctgtggtg atgaagctga aggctgagtt
     3901 gctgaccagg gttgcgccga tgcgctgggc ggtagtgata ttggccgtcg cggtcatgcc
     3961 cgggtagaga tgcgccccgt tcaggctgtt catgtcaacc gacatgacga ccgggtagct
     4021 tacaacgctg gatgaggtct ggccgatgat atccacgctt gagacggtag cgcggaaggt
     4081 cgcgctgggg taggcattga cggtgaactg taccggctgc ccgacctgga ctttgccgat
     4141 atcggcctcg ttgacctggg aagagatggt gaaggaactc aggtcgacca gcacgataaa
     4201 gctcgtcgat gagctactgg aagcgcttga accgccgctg gtgacgttct ggcccacgat
     4261 gccattgacg gccgcaacgg tagcattgcc gggggccgtc atggtggtgg cggccaggtt
     4321 atcctgggcc gtcttgagcg cgagtttggc cgattgcagg ctattgttgt cctggtcaag
     4381 ctggctctgc gacgcg
//
LOCUS       DASZAK010000870         1895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_627643, whole genome shotgun sequence.
ACCESSION   DASZAK010000870 DASZAK010000000
VERSION     DASZAK010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627643"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..551)
                     /locus_tag="VGT44_15270"
     CDS             complement(147..551)
                     /locus_tag="VGT44_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582215.1"
                     /translation="MLPESHIPPKGPVATASDPQPDSHIPPKGPSMTPADATGPDSHI
                     PPKGPDAITADPALADPTGPESHIPPKGPDATPADPTGPDSHIPPKGPSHIPPKGPMA
                     NPTESMNASLELDTSGDQQTAAAIPAIADMAS"
     gene            complement(656..916)
                     /locus_tag="VGT44_15275"
     CDS             complement(656..916)
                     /locus_tag="VGT44_15275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582216.1"
                     /translation="MDQKRKTEALWEQLLSLFPEEREQRLLYLSYNCGLKPAEIARLY
                     PQEFMSIQEIYRLHGSIFDRLQRNADLLPGLMEPGNARYRNM"
     gene            complement(1070..>1895)
                     /locus_tag="VGT44_15280"
     CDS             complement(1070..>1895)
                     /locus_tag="VGT44_15280"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2582217.1"
                     /translation="TRRGHNVRSIMPFLATEHTTMYYDIFQPEFTKDSQNSYQPTVLL
                     IHGFGGTPESDFAGQLPALRQRYRVIAPHLHGYGRSTHRDSYALTFYREDAADLVALL
                     DALEIERVMALAFSDGAIVGLLLAALYPQRVQALAAMGAQPSINAENLSAIRHWLLEK
                     PLHEDWQAELARLHGEPYWRSLPRLYVEGQEALVQAGGIIITGEELAAIRCPTLIMHG
                     QHDRIVPAEYARILHEQVPGSELLLFDAGHAAHLRCEQEYTAAVMEFLDKHSVLVT"
BASE COUNT          349 a          496 c          583 g          467 t
ORIGIN      
        1 gtaagggcgg gggtggagcg gcgtggggtg gagacccttg cggtcgccct cgtcttcctt
       61 gctccgatgt ccgtattatt ttgtcaatgt gcatagtcag cggtggagcg cgatcaatcg
      121 gctcctatgc ctggctgtga ccctcttcag gatgccatgt cggcgatggc tgggatcgct
      181 gcggcggttt gttgatcgcc gctcgtgtcc agttccagtg aggcattcat cgattccgtc
      241 gggttggcca ttggcccctt gggcggaatg tggctcggtc ccttgggcgg gatatggctg
      301 tcggggccgg tgggatcagc cggtgtcgca tccggtcctt tgggtgggat gtggctctcg
      361 ggaccggtcg ggtcggccag agctggatca gctgtgatcg catccggtcc tttgggcgga
      421 atgtgactgt caggaccggt ggcatcagcc ggtgtcatag acggcccttt gggtgggatg
      481 tggctatccg gctgcgggtc tgaggccgtt gctacaggtc ccttgggtgg aatgtggctc
      541 tcaggtaaca tcttcttttt tctcctgttg ctaatcacgc agcgtgttaa gagaactttt
      601 atgtacgaat cgtcaaactt ctacataaga gacgattaca cttaaagttt tttgcctaca
      661 tatttcgata acgtgcgttg ccaggttcca ttaagccggg cagcagatcg gcattgcgct
      721 ggaggcgatc aaagatgctg ccgtggaggc gataaatctc ctggatgctc ataaattcct
      781 gcgggtagag gcgagctatt tcagcaggct ttaacccgca gttataggat agatagagca
      841 gccgctgctc gcgttcttct ggaaacaggc tgagcaactg ttcccacagt gcttcagtct
      901 ttctcttctg gtccattact agctctcctt ctggttcagg caagcgtatc aataggtgat
      961 atcgcactat tcagcacggt acacttataa atgtaccttg ctgcctgcct gcgcgcatcg
     1021 tatgaaccac gtatttgatt acgtattttc tagagtcttt tgcagttttt taagtaacga
     1081 gtacgctatg cttgtccaga aattccatca ccgcggccgt gtattcctgc tcgcagcgta
     1141 aatgggcggc gtgaccggcg tcgaagagca gtagttcaga accaggaacc tgttcgtgaa
     1201 ggatgcgcgc gtattcggcg ggaacaatgc ggtcatgctg gccatgcatg atgagcgtgg
     1261 gacagcgaat ggcggccagt tcttcgccgg tgatgatgat gccgcccgcc tgtacaagcg
     1321 cctcctgtcc ctccacgtac agacgcggca ggctgcgcca gtagggttcg ccatgcagtc
     1381 gcgccagttc cgcctgccag tcttcgtgaa gaggcttttc gagcagccag tgacggatgg
     1441 cgctcaggtt ctccgcgttg atggacggct gcgcccccat ggccgcgagc gcctgtacgc
     1501 gctgtgggta gagcgctgcc agcagcagcc cgacgatcgc gccgtcgcta aaggccagcg
     1561 ccatcacccg ctcgatctcc agggcatcca gcagcgcaac caggtctgcc gcgtcctcgc
     1621 gatagaacgt cagcgcatag gagtcgcgat gcgtcgagcg accatagcca tgcaggtgcg
     1681 gtgcgatgac gcggtagcgc tggcgcagag ctggaagctg cccggcgaag tcgctctcag
     1741 gtgtgccacc gaagccgtga atgagcagca cggttggttg ataggaattc tggctgtctt
     1801 tcgtgaattc tggctgaaaa atatcgtaat acatggtagt atgctcagta gcgagaaatg
     1861 gcataatgct ccttacgttg tgtccgcgtc tcgtt
//
LOCUS       DASZAK010000871         1780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_697381, whole genome shotgun sequence.
ACCESSION   DASZAK010000871 DASZAK010000000
VERSION     DASZAK010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1780
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697381"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VGT44_15285"
     CDS             complement(<1..262)
                     /locus_tag="VGT44_15285"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2582218.1"
                     /translation="MEHALASPEPLLIGRERECTLLWSHYQAAASGQTRVVLIEGPPG
                     IGKTCLLDAMAAHARQQGVCVLRGGASEAEGMPPYLPFLEALG"
     gene            complement(273..>1780)
                     /locus_tag="VGT44_15290"
     CDS             complement(273..>1780)
                     /locus_tag="VGT44_15290"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV2582219.1"
                     /translation="KGRLVPIKDAAGNIVYDQRTRVSRIMALQNDLALVLEAKTIRME
                     APVPGRPYIGVEIPNKNSRLVTLREVLESKEYQAAKAKSRLAVALGKDVAGAVRVGDL
                     ARMPHLLIAGATGAGKSVAINAIIASILTQATPDDVRLLMIDPKMVELNMYNGIPHLL
                     SPVVIEVDRVVALLKVAIAEMEKRYRVFSQLGVRNLDGYRKLRAERLARGDATLKNLP
                     AIVIVIDELADLMMAAPEEVEGLICRLAQLARATGIHLVIATQRPSVDVITGLIKANI
                     PTRISFMVSSAVDSRTIIDMGGAERLLGRGDMLYLPADAGKPERIQGAFVADDEAERL
                     ISYWKQQAADHAAAVAAAANVANSPQNQAGTVVPPAVEPGWEVSDHISDEYELDDELL
                     EQAEQVVYEYERASISLLQRRLRIGYSRAARLIDLLEERGIIGRSEPGGRSREVFERR
                     DARYGGGGEGRTMADEAADIIAEEKAREEFLKKHAGGRSPGQGNGSMGGEE"
BASE COUNT          373 a          561 c          457 g          389 t
ORIGIN      
        1 gaccgagtgc ctccaaaaac ggcagatagg gaggcatgcc ctcggcttcg gaggcaccac
       61 cacgcagcac acacacgcct tgctgtctcg cgtgtgcagc catagcatct aacaggcagg
      121 ttttgccgat tcctggcggg ccctcgatca agaccacgcg tgtctgccca ctggccgcgg
      181 cctggtaatg gctccagagc aacgtgcatt cgcgctcccg cccaataagc agcggttctg
      241 ggcttgcgag tgcgtgttcc atgagcagtc ccctattctt ccccacccat tgatccattc
      301 ccctgtcccg gcgacctacc tcccgcgtgc ttcttcaaaa actcttctcg ggccttctcc
      361 tcggcaataa tatcggctgc ttcgtccgcc atcgttcttc cctcgccacc gcctccgtat
      421 ctcgcatccc ttcgctcaaa cacttcgcga gaacgacctc ctggttcgga acgtccgata
      481 atgccgcgtt cttccagcag atcaattaag cgcgcggccc gcgaatagcc gatacgcagg
      541 cggcgctgca atagcgagat cgaagcgcgt tcatattcat acaccacctg ctctgcctgc
      601 tccagcagtt cgtcatccag ttcatactcg tcagagatat ggtcgctcac ttcccaaccc
      661 ggttcaaccg ctggagggac aaccgtcccg gcctggttct gagggctatt ggcaacattg
      721 gccgcggcag ccacggcggc ggcatgatct gcagcctgct gtttccaata ggagatcaat
      781 ctctcggctt cgtcatcagc aacaaatgcc ccctgaatgc gttcgggctt gccagcatca
      841 gcagggagat agagcatatc accacgacca agcagacgtt cagcaccgcc catatcgatg
      901 atcgtgcgcg agtcaaccgc tgaactcacc ataaaagaga tgcgcgtcgg gatattggcc
      961 ttgatgaggc cggtaataac atctacggag ggacgttgcg tggctatcac caggtgaatg
     1021 cccgtagcac gcgccagttg agctaaacga cagatcaatc cctcaacctc ttcgggtgca
     1081 gccatcatca gatccgccag ttcatcgatg acaatcacaa tggccgggag atttttcagc
     1141 gtggcatcgc cccgggccag tcgttctgct cgcagtttgc gatagccatc caggttgcgc
     1201 acgcccaatt gtgagaagac gcggtagcgt ttctccattt cggcaatggc aactttcaac
     1261 agggcaacga ctctatcaac ttctatcacg accggggaaa gcaggtgcgg aatgccgtta
     1321 tacatattca attcgaccat cttagggtcg atcatcaaca ggcgcacatc atccggcgtg
     1381 gcctgggtga ggatgcttgc aatgatagcg ttgatcgcga cgctcttccc ggctccggta
     1441 gccccggcga tcagcaggtg tggcatgcgc gctaagtcgc caacacgtac cgcgcccgca
     1501 acgtctttac ccagagctac ggccagcctg gattttgcct ttgccgcctg gtactctttg
     1561 ctttccagca cttcccgcaa ggtcaccaac cgactattct tgttgggaat ctcgacgccg
     1621 atataaggac gaccaggaac aggggcttcc atgcggatcg tcttggcttc gagcacgagc
     1681 gcgagatcgt tctgcaaggc cataatacgg ctcacacggg tgcgttgatc atagacgatg
     1741 ttgcctgcag catctttgat aggaaccagg cgcccttttt
//
LOCUS       DASZAK010000872         4650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_271528, whole genome shotgun sequence.
ACCESSION   DASZAK010000872 DASZAK010000000
VERSION     DASZAK010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271528"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VGT44_15295"
     CDS             complement(<1..701)
                     /locus_tag="VGT44_15295"
                     /inference="COORDINATES: protein motif:HMM:NF020933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HEV2582220.1"
                     /translation="MLHKGKLQAALNAKRGQFSADNGAFYEQLQAYRDALETLYLTYT
                     SGEQLEQQLPPASTGLSAGARPSSIEFDRWLVEASSSDYHGPLFPFGRDFANHEQARE
                     WAECIEGITTLAVDGSQLQPWRDASIPVALIQVGLFANPHAQGKPYTKDVQIEVLAPG
                     EIMEQSRTEEKDPDSYPYSEVEVTLRRYTLEVETLCRQMEQYARNRSKHDRYGSDPAY
                     SPVVFFDGSLVVSFAP"
     gene            complement(691..3807)
                     /locus_tag="VGT44_15300"
     CDS             complement(691..3807)
                     /locus_tag="VGT44_15300"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEV2582221.1"
                     /translation="MLITRIELENIKSYRHLTLDFRRGTTAISGVNGAGKTTLVEAIG
                     YALFNHLPYNQSQFVREGEKTGKIVVHLLGSDDRPYEVERRCGASAQWSVFDVEANFR
                     VEQRADVQDKLHDIFGIDRERPLESLFRDALGVPQGTFTAIFLEPARIRKETFNALLQ
                     IEDYETAFKYLLQVSNHYKEQVQEQKSEIQRLEIETRELPEWRATLKERRADDEQMKQ
                     QNVQYTEQLVVCEARSQLLKQQREQLNATRSAYAQQQTKAAAARQWLDSCAQSLRDAR
                     QAQQAIEASENDHARYTQADALLRQLRQDERLRNSLLEQSNQHRHGLATIEANIRNLL
                     TQLDEVETARKRLAELAPFVEQQYELDMQRETLQRQALQHEGLVKDIKRLDGQQISSQ
                     QKLDGLQRAIAAIEPLQPLAAQFNERQERYTALQVQAAERSTKRRAFTEKNDLLQKKL
                     DEKEQAATNLRKAEDFIAALEEHRAEAEELLDLQTQSLDLTAQVNRLKGNIDGYTKSR
                     RLSAGGQCPLLHEPCQNIKARGLISLEFYFDDEIKRDKAEIDALNQRLSTVDKRATQV
                     KRYADELSKIGFYTEKRDSYAERLRQVALEITHLEREISALQADLDALNDIDAQIAQA
                     RIDQEESQRADRQMRTLPGLQQQSTEIQEQFAQTIADIRERGQQAELLRDSAAQQKIV
                     EHELKQLNDPRSQSKVQQATIQREEEYQQQLRREQAQQQQSEQRLQELQQQLAQYAIL
                     DARIAEQEAIRAMCQQGYHTYLKNQDIARQLPERELAEQQARITSEQTDQDLQAAESA
                     YNEAKAAFNEEELLAIDKQITFLNREISAIARELSNLQEKINDLEHKIELGDALLIEL
                     EAARKEKATLEDLTRMVDQFRKLIKEAAPFVLKAMLTDISGEANRIFCEIMGDRSAQL
                     NWLDDYEIVLRRQGIDRTFAQLSGGEQMSAALAMRLALLKKLSSLNIAFFDEPTQNMD
                     ELRRTNLADQIRRVRGFDQLFVISHDDTFEQSLDSLVRLRKKDGETHIVAEDETISER
                     EQVTVNAS"
     gene            complement(4158..>4650)
                     /locus_tag="VGT44_15305"
     CDS             complement(4158..>4650)
                     /locus_tag="VGT44_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582222.1"
                     /translation="HILQYPGLDGWARSIPTADGGVLERLRRLSDRLAEKYKWQHGQA
                     TAFVLTGGVPLISPIIITVSTNSRPLFSRIMLDVDPALSPKELILYYRRNRRKTLTAR
                     HRNLSEKHMQLALFDSRRPKAETWVIKMDEWNKIHQTEWRYKKVSNFAHDCKQARRRL
                     LRS"
BASE COUNT          768 a         1270 c         1406 g         1206 t
ORIGIN      
        1 ggggcgaagg agaccaccag cgagccatcg aagaagacca ccgggctgta ggcggggtcg
       61 ctgccgtagc ggtcgtgctt gctgcggttg cgcgcgtatt gctccatctg gcggcagagc
      121 gtttcgactt cgagcgtgta gcggcgcagc gtgacttcca cctctgaata gggatagctg
      181 tcggggtctt tttcttcggt gcgtgactgc tccatgatct cgccgggcgc gagaacctcg
      241 atctgcacat ctttggtgta gggtttgccc tgggcgtgtg ggttagcgaa cagccccacc
      301 tggatgagcg cgacagggat cgaggcgtcg cgccagggct gcaactggct gccatcgacg
      361 gccagggtcg tgatgccctc gatgcattcc gcccattcgc gggcctgctc gtgattggcg
      421 aagtcgcgtc caaaggggaa gagcgggccg tgatagtcgc tgctggaggc ttcgaccagc
      481 cagcgatcaa attcgatgct gctgggccgc gcgcctgccg agagtccggt tgaggcggga
      541 ggtaactgct gctcaagctg ctcgcccgac gtgtaggtca ggtagagcgt ttcgagcgcg
      601 tcgcgataag cctgcaattg ctcgtagaac gcgccgttgt cggcgctgaa ctggccgcgt
      661 ttggcgttca gggcggcctg gagtttgcct ttatgaagca ttgacagtga cctgctctct
      721 ctcggaaatg gtctcgtcct ccgcaacgat gtgcgtctct ccgtcctttt tgcgcaggcg
      781 caccaggctg tcgaggctct gctcgaacgt gtcgtcgtgg ctgatgacga agagctggtc
      841 gaaaccgcgc acacggcgaa tctggtcggc gaggttggtg cggcgcagct catccatgtt
      901 ctgcgtcggc tcatcgaaaa aggcgatatt gagcgaggag agtttcttga gcagcgccag
      961 gcgcatggcg agcgcggcgc tcatctgctc gccaccgctc aactgcgcga aggtgcggtc
     1021 gatgccctgg cggcgcagca cgatctcgta atcatccagc cagttcaact gcgcgctgcg
     1081 gtcgcccatg atctcgcaga agatgcggtt ggcctcgccc gaaatgtcgg tgagcatagc
     1141 tttcaagacg aatggagcgg cctctttaat cagcttgcgg aactggtcaa ccatcctggt
     1201 caagtcctca agcgtagctt tttctttcct ggccgcttcc agttcaatca agagggcatc
     1261 gccgagttct attttgtgtt cgagatcgtt gattttttcc tgcaagttgc tcaattcgcg
     1321 cgcgatggcc gaaatctcgc ggttcaggaa agtgatctgt ttgtcgattg cgagcagttc
     1381 ttcctcgtta aaagcggcct tcgcctcgtt gtaggcgctt tcagccgctt gcaggtcctg
     1441 gtcggtctgc tctgatgtga tgcgcgcctg ctgttctgcc agttcgcgtt cgggaagctg
     1501 gcgcgcgata tcctgattct tgagataggt atggtagccc tgctggcaca tcgcgcgaat
     1561 ggcttcctgc tccgcgatgc gcgcgtcgag aatggcgtac tgtgccagtt gctgctgtag
     1621 ctcctgaagt cgctgctcgc tctgctgctg ttgcgcctgt tcgcgtcgca attgctgttg
     1681 atactcctcc tcgcgctgaa tggtcgcctg ctgtaccttg ctctgcgagc ggggatcgtt
     1741 cagttgcttc agttcatgct cgacgatctt ctgctgcgca gcactgtcgc gcaatagctc
     1801 ggcctgctgc ccgcgctctc gaatatcggc gatggtctgt gcgaattgct cttgaatttc
     1861 agtggattgc tgttgcagtc cgggcagggt gcgcatctga cggtctgcgc gctggctctc
     1921 ttcctggtcg attctggcct gcgcgatctg cgcatcgata tcgttgagcg cgtccaggtc
     1981 tgcctgcaaa gcgctaatct cgcgttccag gtgggtgatc tccagcgcta cctggcgcag
     2041 gcgctcggca tagctgtcgc gtttctcggt ataaaaaccg atcttgctca attcgtcggc
     2101 gtagcgtttg acctgggttg cgcgtttatc aaccgttgag agtcgctggt tgagcgcgtc
     2161 tatctcggcc ttatcgcgtt tgatctcgtc gtcgaaatag aattcgaggc tgatcagccc
     2221 gcgcgccttg atgttctggc agggttcgtg cagcagggga cactgcccac cggcagagag
     2281 cctgcgagat ttcgtatagc catcgatatt gcccttgagc cggttgacct gggcggtcag
     2341 gtcgagcgat tgcgtttgca ggtcgagcaa ttcttcggct tccgcgcgat gttcttccag
     2401 cgcggcgata aaatcttcgg ctttgcgtag attggtggcg gcctgctctt tctcgtccag
     2461 ttttttctgc aacaggtcgt ttttttcggt gaaggcgcgt cgctttgtgc tgcgctccgc
     2521 agcctgtacc tgcaaggcgg tgtagcgttc ctggcgttcg ttgaactgcg cggccagggg
     2581 ctgtaatggt tcgatggcgg cgatggcgcg ctggaggcca tcgagctttt gctgcgagct
     2641 gatctgctgc ccatccaggc gtttgatgtc cttgaccagc ccttcgtgct ggagcgcctg
     2701 tcgctgcaag gtttcgcgct gcatgtccag ttcatattgc tgctcgacga agggggcaag
     2761 ttcggcgagg cgtttgcgcg ccgtctctac ttcatccagt tgggtgagca ggttgcggat
     2821 gttggcttcg atggtggcga ggccgtgacg atgctggttg gattgctcta acaggctgtt
     2881 gcgcaggcgt tcgtcctggc gcagctggcg cagcagggcg tcggcctggg tgtagcgcgc
     2941 gtggtcattc tcgctcgctt cgatggcctg ctgggcctgc cgggcgtcgc gcagcgattg
     3001 cgcgcagcta tccagccatt gtcgcgccgc cgctgccttc gtttgctgct gggcataggc
     3061 ggagcgggtc gcgttgagct gctcgcgctg ctgtttgaga agctggctgc gcgcctcaca
     3121 cacaacaagc tgttcggtat attggacgtt ctgctgcttc atctgctcat cgtccgcgcg
     3181 ccgttctttg agcgttgcgc gccattcggg cagctcgcgc gtctcgattt ccaggcgctg
     3241 gatttcgctc ttctgttcct ggacctgctc tttatagtga ttgctgactt gcagcaggta
     3301 tttgaaggcg gtctcgtagt cttcgatctg gagcagggcg ttgaaggttt ccttgcggat
     3361 gcgggcaggt tcgagaaata tggcggtgaa ggttccctgg gggacgccca gggcatcgcg
     3421 gaagaggctt tctagcgggc gttcgcggtc gatgccgaag atgtcatgca gcttatcctg
     3481 tacatcggcg cgctgctcaa cgcgaaaatt ggcctcgaca tcaaagacgc tccactgcgc
     3541 cgaggcgccg cagcgccgct cgacttcgta ggggcggtca tcgctgccga gcaggtggac
     3601 gacgatcttg ccggtctttt cgccctcgcg cacaaactgg ctctggttgt agggcaggtg
     3661 gttgaacagg gcgtagccga tggcctcgac cagcgtggtt ttgcctgcgc cgttgacgcc
     3721 gctgatggcg gtggtgccgc gcctgaaatc gagggtgagg tgacgataac tcttgatgtt
     3781 ctccagttcg atgcgagtga tgagcatgat aacgcgcctt cccaacaaat atgagtgagt
     3841 tgcccacttt ccttattctc ccgcttgcat gagaaagtgg aaatgtacaa ggcgcattat
     3901 aacacgtgcg ggcaacagat tttctcatct tcgctgattg gcttcttgag cggctcggcg
     3961 attattgcat tatgcagctt tgtctactga tgtctaccat gttcactcgt tttccaccgg
     4021 ttaagctatc aactttgctg tcaacttctg gcctcacgcg cttgaatttt tcagccttct
     4081 tgatcagttt agcgaatacc ttattaagcc tttctatttg ttccacgtag tcacattttc
     4141 acgttttcga gcctcgctta agaacgcaat aatcgacgcc gcgcttgttt gcaatcatgg
     4201 gcaaaatttg acaccttctt atatctccat tcagtctggt gtatcttatt ccattcatcc
     4261 atctttataa cccaggtttc agcctttggc cgccttgaat caaagagagc aagttgcata
     4321 tgtttctcgc ttaagttccg atgccgcgca gtcaaggtct tcctacggtt tcttctgtaa
     4381 tacaggatta attctttcgg tgacaaagca gggtccacat cgagcatgat tcgtgaaaag
     4441 agcggacgag aatttgtact cactgtgata attatagggc taattagtgg aactccgccg
     4501 gtgaggacga aggcagtagc ttgcccatgc tgccatttgt acttctcggc taaccggtcg
     4561 ctcaaccttc ggaggcgctc caatacaccg ccatcagcgg ttggtatact acgcgcccat
     4621 ccatcaagcc ctggatattg caagatatgc
//
LOCUS       DASZAK010000873         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_341674, whole genome shotgun sequence.
ACCESSION   DASZAK010000873 DASZAK010000000
VERSION     DASZAK010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341674"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VGT44_15310"
     CDS             <1..352
                     /locus_tag="VGT44_15310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582223.1"
                     /translation="VHLVGTLTFDAGSLDFPYMEKMVNDLLDPLYARVENNTNDQDYI
                     PENGEIDGRDRATWHELERHIFEDLVSRDNRYLVHREQWSHVLADLKRMALDEDNPVE
                     IARFLREKRVELMN"
     gene            complement(360..995)
                     /locus_tag="VGT44_15315"
     CDS             complement(360..995)
                     /locus_tag="VGT44_15315"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEV2582224.1"
                     /translation="MSWRLASRLSVVTLALLVVLVVAFFSLRNITNGQSLPGNPATNT
                     DGSQGTDLGSVTAPDFHLTDQFGKQVSLSQFKGEPVVLTFLYTHCPDVCPLIAEQLHT
                     VMLDLGKNAQHVAVLAVSVDPGRDTIASALAFSKTHNMTAYWHYLVGTEAQLSPVWTA
                     YAIGAQQETSTVSMHTTALYIIDKQGRERILLDQTFTPAYLTGLLQTLLKE"
     gene            1347..1838
                     /locus_tag="VGT44_15320"
     CDS             1347..1838
                     /locus_tag="VGT44_15320"
                     /inference="COORDINATES: protein motif:HMM:NF025698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HEV2582225.1"
                     /translation="MERASASAAAIPRVDTHLAKFSQAGTVLLTGLAFIINQPVIVLV
                     AAVVMTLAALLPEYSPYRLVYRGIVLPLKLWQPRVVEDDPAPHRFAQGVGAVFLIAAT
                     LVLFLTSATALGWTLDLIVFVLAGINLTVGFCAGCFVYYHLGRLGALPRVRYEGGFHW
                     RGV"
     gene            1842..2318
                     /locus_tag="VGT44_15325"
     CDS             1842..2318
                     /locus_tag="VGT44_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582226.1"
                     /translation="MTALLLRIGILALVGLLTWAIVWSGRSFVERRRFVAMTSGTARD
                     IALTGQIELQAGSASSSPVHILAFSSADCHQCHLYQMPALRRVTEARMDMVSVEEIDA
                     PTSPELTRRYQVLTVPTTVVLDASGRVQAINYGLANTQRLLEQVDAILATEMSVPS"
BASE COUNT          480 a          616 c          742 g          543 t
ORIGIN      
        1 ggtccacctg gtcggcacat tgaccttcga tgcgggttcg ctcgattttc cctatatgga
       61 gaagatggtc aacgatctgc tcgacccgct ttacgcgcgc gttgagaaca ataccaacga
      121 ccaggattac atcccggaaa atggcgaaat cgatggccgc gatcgtgcta cctggcacga
      181 actggaacgt cacatctttg aagacctggt cagccgcgac aatcgctatc tcgttcaccg
      241 cgagcagtgg agccatgtgc tggccgacct gaaacgcatg gcgctcgacg aagacaaccc
      301 ggttgagatt gcgcgcttct tgcgcgagaa gcgggtggag ttgatgaatt aagcctcatt
      361 tattctttca acagcgtctg caacagcccc gtcaggtaag caggggtgaa ggtctggtcg
      421 agcagaatgc gttcgcggcc ctgtttgtcg atgatgtaca gagcggtggt atgcatactg
      481 acggtggagg tttcctgctg cgcgccgatg gcgtaggctg tccagacggg agagagctgc
      541 gcttcggtgc caaccaggta atgccagtaa gcggtcatat tgtgtgtctt ggagaaggcc
      601 agcgccgagg cgatggtgtc gcgccccgga tcgacactga cggcaagcac ggcgacatgc
      661 tgcgcgttct tgccgaggtc gagcatgacg gtatgcaatt gttcagcgat taaggggcag
      721 acatcggggc aatgcgtgta gaggaaggtg agtacgacgg gttcgccctt aaactgcgat
      781 agagagacct gcttgccgaa ctgatcggtc aggtgaaaat cgggcgcggt cacgctgcca
      841 aggtcggttc cctgcgaacc atcggtgttg gttgccggat tgccgggaag cgactgtcca
      901 ttcgtgatgt tgcgcaggct gaagaacgcc acgaccagca ccacaagcag ggccagcgtg
      961 acgacggaga gccgagaagc cagtcgccaa ctcatatatt tcaatcatcc tagctgaaaa
     1021 taaaaacacc acaggggcta caagggcgac cgcaagggta cacccttaca atgattcctg
     1081 cccgtattgt tgcgaacatt cctgattttc tgcttgcaat tggcgcagta tgccttgcag
     1141 ttgcctgaat gggcgatgag gtatcctcag ggctgatgca aagtcgagag aggcatggcg
     1201 cacccttgcg gtcgtcatgc ctctctcgat tggttctttc cttgacatga gtattttcta
     1261 tgcgctacac ttatacataa gttgatttag tttatcaact tagataacaa tggtttcatg
     1321 gcattattcc gaaggagtta tgcgtaatgg agagagcatc cgcgtctgcc gctgcgatac
     1381 cgcgtgtaga cacacacctt gcgaagtttt ctcaggccgg gacggtgctg ctgaccgggt
     1441 tggcctttat cataaatcag ccggtgattg tgctggtggc ggcggtggtc atgacgctgg
     1501 ccgcgctttt gccggagtat agcccgtacc ggctggtata tcgcggcatc gtgctgccgc
     1561 tcaagctctg gcagccgcgt gttgtcgagg atgatcccgc gccgcatcgc ttcgcgcagg
     1621 gggttggcgc ggtttttctg atcgcggcga cgctggtgct tttcctgacg agtgcaacag
     1681 cgctgggctg gacgcttgat ctgatcgtgt ttgtgctggc gggcatcaat ctgacggtcg
     1741 ggttttgcgc gggctgtttt gtttactatc acctgggacg gctgggggcg ctgccgcgtg
     1801 tgcgttacga aggcggcttc cactggcgag gagtataaca cgtgacggcc ctactcttgc
     1861 gcatcggcat tcttgcgctg gttggcctgc tgacctgggc gattgtctgg tcgggtcgga
     1921 gctttgttga acggcgtcgc ttcgtggcga tgacgagtgg aaccgctcgc gacattgccc
     1981 tgaccgggca aatcgaattg caggccggga gcgcgtcatc gtcacctgta catattctgg
     2041 ctttcagcag cgctgactgt caccagtgcc acctgtatca gatgcctgct ctgcggcgcg
     2101 taaccgaggc gcgtatggat atggtgagtg tggaagaaat cgacgcgccc acctctcccg
     2161 aattgacgcg gcgctaccag gtgctgaccg tgccgacaac ggtcgtgctg gacgccagcg
     2221 ggcgggtaca ggccatcaat tacgggcttg ccaatacgca gcgcctgctt gagcaggtgg
     2281 acgcgatatt ggcgacggaa atgtcggtac cttcgtaggg acgcccagaa caagagaagg
     2341 agttttcgat gtcagaagct cagaattttc gcaaggccaa t
//
LOCUS       DASZAK010000874         1836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_450043, whole genome shotgun sequence.
ACCESSION   DASZAK010000874 DASZAK010000000
VERSION     DASZAK010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450043"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..281)
                     /locus_tag="VGT44_15330"
     CDS             complement(72..281)
                     /locus_tag="VGT44_15330"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV2582227.1"
                     /translation="MARQLAIENEHIAADVIEISEFMDLAQHYCVQGIPKTVINDRVE
                     FVGALPEPGFVQQVLQTLDKGQQGT"
     gene            complement(229..468)
                     /locus_tag="VGT44_15335"
     CDS             complement(229..468)
                     /locus_tag="VGT44_15335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582228.1"
                     /translation="MRFFGIPAGYEFSWLVEDLLDLSTGTTKLSEKTRTALQEIDQDL
                     HIQVFVTPTRPLLPICGQVGPSVGNRKRAYCGRCD"
     gene            complement(840..1007)
                     /locus_tag="VGT44_15340"
     CDS             complement(840..1007)
                     /locus_tag="VGT44_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582229.1"
                     /translation="MAAYQVTDGKEISTGLLSASEVGMHLVGWLPPGKDDQRAAQLAA
                     QIGIQILPIST"
     gene            1238..1390
                     /locus_tag="VGT44_15345"
                     /pseudo
     CDS             1238..1390
                     /locus_tag="VGT44_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004514800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1389..1553
                     /locus_tag="VGT44_15350"
                     /pseudo
     CDS             1389..1553
                     /locus_tag="VGT44_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          387 a          522 c          505 g          422 t
ORIGIN      
        1 gggacacaac ccattgaggc caaacctggc cactctcggc gaaatatgga ttgcacttta
       61 gcagagcagg tttatgttcc ttgttgcccc ttgtcaagcg tctgcaagac ctgttggaca
      121 aagccaggct ccggtaatgc cccgacaaac tcaacgcggt cattgatgac ggtttttggt
      181 atgccctgga cgcagtagtg ttgggcgaga tccataaact ccgagatttc aatcacatcg
      241 gccgcaatat gctcgttttc gattgccaac tgacgggcca acctggccgc agatgggcag
      301 tagggggcgc gtcggggtca caaagacctg gatatgcagg tcctgatcga tctcctggag
      361 ggccgtgcgt gtcttctcgc tcagtttcgt cgttcctgtg gagagatcga ggagatcttc
      421 caccaaccag gaaaattcat agcccgccgg gatgccgaaa aagcgcacct tccccctggc
      481 ccgcccctga taccaattcg caatctgtcg tataaagaaa ggggacctcc ttcgttttca
      541 taccatcacc gaacctgttt ttcaacaagc ttcgaccgat tctgcaacac atcaatcgga
      601 ctgccaaatg cgcttggaag cagcttccac gccgctctgc aggtctcgcg ctcctttcgc
      661 tgcccgcagc ccgttcgttg tgctggtgcg aggctgcctg tctggcagga gagaagggga
      721 aagcgcgatc agagctgttc gagtactgcc tctaggtttt tcactcctcc tggaatagcc
      781 tcttcgtcgg tcccggcata gccaaaggcc agcccccgcg agacagcggc tccaggctat
      841 taggtagaga tgggcaggat ctgaataccg atctgcgcgg ccaattgggc ggcgcgctga
      901 tcatctttgc caggcgggag ccagccaacc aggtgcatcc cgacctcgga agcggagagc
      961 aagcctgtgc tgatttcctt tccatccgtg acctgatacg ccgccatgag cgttcggctg
     1021 aggaggtctg gcagttcgtg cgcaccggcg actgtctgcc gtatcggagg cgagccggag
     1081 ggcgctggga gtggcgcttt tgcccacaga attgcaagag tgcgtagcca tcagatacaa
     1141 tcagctccag gatgatcgtc atgccatgga tcgttttcga tgtcgagttc gagctctgag
     1201 gaagagaggg acgcattatg actagcgaag taccgcccgc tatgagcatg gctcgatcat
     1261 cctgacttct aataccagct ttgctgggtg gggacggttg ctcggtggag acgaggtgct
     1321 gaccacggca ctgctcgacc ggctgcttca tcacgcggag gtgatttcca tcaacggtcg
     1381 cagctaccgc tggcatccgg cgcgattatg ccgccgtgcg cgccaccttc acttcccagt
     1441 ggagtaatgg tccagttgaa tcgcaggtca actgcttgaa gctgcaaaaa cggctgatgt
     1501 tcggccgggc caattttgac ttattgcggc tccatgtctt gcgtcgcgcc taagtgctct
     1561 cttctcttcc tcgccattct ccctctgatc ctcttctcaa ccacaagcaa ccaagctcac
     1621 ttttttcgag gatgagccac tctaatggcg tatccccaaa gttggtacag aagttattgg
     1681 ggcacagcaa catttcgatc gcgatggacc gctattggca tgttcttccc tcgatgcaaa
     1741 aggagatggc acagcaactg gatagcctgt ttgggaatgg cgaacagaat caatgagtcg
     1801 tctctactat ctatcaacta ccagcgcata gcgacg
//
LOCUS       DASZAK010000875         1758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_378604, whole genome shotgun sequence.
ACCESSION   DASZAK010000875 DASZAK010000000
VERSION     DASZAK010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378604"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1056
                     /locus_tag="VGT44_15355"
     CDS             85..1056
                     /locus_tag="VGT44_15355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEV2582230.1"
                     /translation="MKAIRIHETGGPEVMRLEEVDTPTPAQGEVLLKIAAAGINYADI
                     AQRRGAYLTRTITPMTLGFEAAGTVAAHGPGVSSPPIGTRVVAFVNGGYAEYAISSPT
                     NLIPIPDSLDFVHAAAFPVQGLTAYQTLRESGRLQPGESVLVQAAAGGVGTLAVQLAR
                     LMGAGKIIGTASSEQKLELVRSLGADAAINYTQDDWVEQVKQATGGRGVDIVLEVVGG
                     KITEQSLGCMAPFGRLVVIGAASGERPQFSGVQLMYKNLSVVGYWLTAWLNRPDRIAA
                     ATIELMQYLASSSLRIIVGQTYPLAEAADAHRAIASRTTTGKVVLLV"
     gene            1068..1727
                     /locus_tag="VGT44_15360"
     CDS             1068..1727
                     /locus_tag="VGT44_15360"
                     /inference="COORDINATES: protein motif:HMM:NF036807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEV2582231.1"
                     /translation="MTEQLDGSRPSNGLKLIGAGFGRTGTYSLKNALEELGFSPCYHM
                     TTLFENEGTVEQWDAIVRGATPDWQSVFAGYQATVDWPACTFYQDLVRAYPDAKVLLS
                     VRDPEKWYESVATTIYQTSNRDLTSPHARMIDRLIWQGTFKDRFLEKDEAIAIYQRHN
                     EEVKQFVPPEKLLVYDVKQGWEPLCAFLGVPTPSNTPFPHLNDRESFLNRTRPQPGPG
                     Q"
BASE COUNT          388 a          571 c          496 g          303 t
ORIGIN      
        1 ctcaccagcg acgagggcgc aatgaattgc gcccctacag gttagctcaa tcttcgaata
       61 cattacgcac gaaagggaca tcatatgaaa gccattcgta ttcatgaaac gggcgggccg
      121 gaggtgatgc gcctcgaaga agtggacacc ccgacacctg cgcaggggga agtcctgctc
      181 aaaatcgcgg cggcgggcat caattacgcc gacattgcgc agcggcgggg agcctatctc
      241 acgcgcacaa tcacgcccat gacactggga ttcgaggccg ccggcactgt cgccgcgcat
      301 ggcccaggcg tcagttcacc gcccatcggc acgcgcgtcg tggcattcgt gaacggcggc
      361 tacgctgaat acgccatctc cagccccacg aacctgattc ccattcccga tagcctcgac
      421 ttcgtccacg ccgccgcctt tcccgtacag ggactgaccg cctaccagac cctgcgcgaa
      481 tcgggccgtc tccagccggg cgagagcgtc ctggttcaag cggcagccgg gggagtcggc
      541 acgctcgccg tgcaacttgc ccgcttgatg ggcgcgggca agatcatcgg caccgccagc
      601 agcgagcaga aacttgagct tgtgcgcagc ctgggcgctg acgcggccat caactatacc
      661 caggacgatt gggttgagca agtcaagcag gccacaggcg ggcgcggcgt tgatatcgtc
      721 ctcgaagtgg ttggcggcaa aatcaccgaa cagagcctcg gctgcatggc tccctttggc
      781 cgcctcgtgg tgattggcgc ggccagcggc gagcgcccgc aattctccgg cgtccagctc
      841 atgtacaaaa acctctccgt cgtcggctac tggctaaccg cctggttgaa tcgccccgac
      901 cgcatcgcag cagcgaccat cgagttgatg caatacctgg caagcagctc gctgcgcatc
      961 atcgtcggtc agacctaccc gctggccgag gcagccgacg cgcaccgcgc catcgcctcc
     1021 cgcacaacaa ccggcaaagt cgtactgtta gtttgaagga gatgactgtg acagagcaat
     1081 tggacggatc gcgcccatcg aatggactga agctgatcgg cgctggcttc gggcgaaccg
     1141 gcacctattc cttgaagaac gcgctggagg aactcggctt tagcccatgc taccatatga
     1201 ccacgctctt cgagaatgaa ggaacggtag aacagtggga cgccatcgtc aggggagcta
     1261 cacccgactg gcagagcgtc ttcgcgggct atcaggcaac ggtagactgg cccgcctgca
     1321 ccttctatca agacctggtg cgcgcatatc ccgacgcgaa ggtgctgctt tcggttcgcg
     1381 accctgaaaa gtggtatgaa agcgttgcca ccaccatcta ccaaacttcc aaccgtgatc
     1441 tcacttctcc acatgcgcgc atgatcgata ggctcatctg gcaaggcaca ttcaaagaca
     1501 gatttctgga gaaagacgaa gccatcgcta tctaccagcg ccacaacgag gaagtcaagc
     1561 agttcgtccc gccggaaaag ctgctcgtct acgacgtcaa acagggctgg gagccgctct
     1621 gcgccttcct gggcgtccca actccgtcga atacaccgtt cccacatctc aacgaccgcg
     1681 agagcttctt gaacaggacc cgcccgcaac caggcccagg ccaataaagc cattcaagcc
     1741 ttgctcaagg ccacgaga
//
LOCUS       DASZAK010000876         4730 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_341711, whole genome shotgun sequence.
ACCESSION   DASZAK010000876 DASZAK010000000
VERSION     DASZAK010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4730
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341711"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VGT44_15365"
     CDS             complement(<1..384)
                     /locus_tag="VGT44_15365"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEV2582232.1"
                     /translation="MTDKLKAAYKTDVGRQREQNEDYPYVFITEEQERGLFIVADGMG
                     GYRAGEVASKMAVEKMRESLNNLLIPISEQPTIKLNPVADQETVILDATRAAELAKLP
                     KTRKLTETNQLKNIEEQLKNAVRNAN"
     gene            complement(670..1035)
                     /locus_tag="VGT44_15370"
     CDS             complement(670..1035)
                     /locus_tag="VGT44_15370"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2582233.1"
                     /translation="MDEINDGAWYVISVAAIKADVHEQTLRHYERLGLISPARKGVSP
                     HSPRLYSDRDIERVMHIRRLMRDLGVNLAGVEAILHMKDRMEHAQREQDEQIRQIHEQ
                     HEVEMRRLKEIIERLQRGL"
     gene            complement(1049..2077)
                     /locus_tag="VGT44_15375"
     CDS             complement(1049..2077)
                     /locus_tag="VGT44_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEV2582234.1"
                     /translation="MEYKDYYKILGVAKSASAEEIKKAFRKLARKYHPDVNPGDKKAE
                     EKFKEINEAYEVLSDAEKRRKYDTLGPNWMDQFGPSAGYGTGRRASGASGGFRSSPFN
                     FDTSSNFSDFFETLFGNTPGSGSRASGTRTGDDIRRRVGDNIDQPVEVTLQEAYAGGT
                     RTFNVQSTEICPICRGLGEIGGKPCANCDGQGQLVRSKRIQVKIPAGVDNGSRIRVAG
                     EGQPGIGGAPRGDLFLVISVKPDTVFERKGDDLHLDFEVDLVTAMLGGEAPVPLIDGR
                     KVTMTIPPETQNGRVFRLAGKGMPHLRGEGNGNLFARLKIVLPMRLTAEERALFEQLA
                     RIRGATPV"
     gene            complement(2160..2720)
                     /locus_tag="VGT44_15380"
     CDS             complement(2160..2720)
                     /locus_tag="VGT44_15380"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV2582235.1"
                     /translation="MQDSQEMQGGRMTEESTTGAPISIDERIAALEQQLAQARQEATE
                     NWNKYLRERAEMDNFRKRQMKMGMERAQQEKARLLNKVLEVVDNIDRAMLYQDKMERQ
                     ELLQTMRMLMWHLNEVLRNEGLEPIATVGQVFDPRVHEAIESVEDSNQPEGTVIEEVL
                     KGYKLGNDTFRPARVKVSAGRKEAQA"
     gene            complement(2814..3686)
                     /locus_tag="VGT44_15385"
     CDS             complement(2814..3686)
                     /locus_tag="VGT44_15385"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEV2582236.1"
                     /translation="MLDSVEKEIQEGLLQTAVAGEQGLADNEHSNYVVRLPIFEGPLD
                     LLLHLIEKRQMEITTVSLVAVTDQYMAYLRQWREDNMPLANMAAFLSIATRLLFIKSQ
                     SLLPRTTKEEMTSEMESAAAMAEELRAHLLEYKLAKEIARYLRLREKAGLQTFARSGL
                     LAGIEAQLAWTPPTIVGLEVETLAQAFARLLAQQAREQVNGIAFVPTARVRVSERIAE
                     IIDRLRARPRVLLSELLEEAPSRVIIIVTFLAVLELWKHARIAVVQDLLFSPIALERG
                     EKWREDAPLESEGW"
     gene            complement(3690..4199)
                     /locus_tag="VGT44_15390"
     CDS             complement(3690..4199)
                     /locus_tag="VGT44_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582237.1"
                     /translation="MAAFAYRLWQVSQQLGFVSPLAEDERREVARWLPESAYALFTSM
                     SPADQRHALRVCQGLQARGCAEPDMLAAALLHDVGKAQGRVPFWTRPVIVVGKRFAPR
                     LLQQVAVYPRDGLPRWRRALGYAWWHAEVGADMAAGAGLPEHAVQYIRAHHQPGGPAA
                     ELHLVDEAS"
     gene            complement(4221..>4730)
                     /locus_tag="VGT44_15395"
     CDS             complement(4221..>4730)
                     /locus_tag="VGT44_15395"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2582238.1"
                     /translation="WGNPVKADPWKLRGGPNVGTLIVALGGIFTSLLIGGIFAVVLRV
                     LPGTLYLNAFAVRIPQLVATFASVNICLAIFNLIPLYPLDGYQILYSLLPSRQAVSFA
                     KSASYGPFIILLLIFLLPFLGQLVGLSDFFLFRISYYILQGSMQLIGLITGFGGIGAY
                     AIYLGPPVI"
BASE COUNT          942 a         1435 c         1279 g         1074 t
ORIGIN      
        1 gttggcattg cgcaccgcgt tctttaactg ctcctcaata tttttcaact gattggtctc
       61 ggtaagcttg cgggtcttcg gtaatttggc tagctcagcg gccctggtcg cgtctaaaat
      121 gaccgtttcc tggtcggcta caggattgag cttgatagtt ggctgctcag atatgggtat
      181 cagcaggttg ttgagcgatt ctctcatctt ctcaacggcc attttactgg caacctcacc
      241 ggcgcgatag ccccccattc catcggcgac gatgaagagg ccacgctcct gctcttctgt
      301 gataaacaca tacgggtaat cttcgttttg ctcgcgctgt cgtccaacat cggttttata
      361 agcggccttt aatttatctg tcataggttt tccttcacag caacgtctgt tcaacgttta
      421 ttttaaatca atattagcat atcgcggttc tcatagcaat aaactggctt atatagcgcg
      481 atggcaatac gtctcgccat cttttcaata acatgcgctc cctttattca tttgtatatc
      541 tgaatcatct acgagcggat aaatcggttt gttgcaacca gctcacccac cgtagttgtc
      601 tgtcattctt catttagcct gtaggggcgg gggaagcgga cgggccgggt agggacgctt
      661 gcgtcgcccc tacagaccgc gttgcagacg ctcaatgatc tccttgagcc ggcgcatctc
      721 aacttcatgc tgctcgtgta tctggcgtat ctgttcgtcc tgctcgcgct gggcatgttc
      781 catgcgatct ttcatatgca agatggcctc taccccggcc aggttaacac ccaggtcgcg
      841 catcaaacgc cggatatgca tgacgcgctc aatatcgcga tccgagtaga gtcgtgggct
      901 gtgcgggctg acccctttgc gagcaggcga gattagcccc aggcgctcat aatggcgcag
      961 cgtctgctca tgtacatcag ctttgatggc agcgacactt atgacatacc acgcaccatc
     1021 attgatttcg tccacgattg cgctctcctt ataccggcgt cgcgccgcga atgcgggcca
     1081 gttgctcgaa caatgcgcgc tcttccgcgg tcaggcgcat aggcagcacg atcttcaaac
     1141 gcgcgaaaag attgccgttg ccctccccgc gcaggtgagg catgcccttg cccgccaggc
     1201 ggaagacgcg cccattctgc gtctccggcg gaatagtcat agtcactttg cgcccatcaa
     1261 tgagcggcac cggcgcttcc ccacccagca tagccgtcac caggtccact tcaaagtcga
     1321 gatgcaggtc atcgcctttc cgctcgaaca ccgtatcggg cttgacgctg atcaccagga
     1381 aaaggtcgcc gcgcggcgct ccgcctatgc ccggctgccc ctctcccgcg acgcgaatcc
     1441 ttgagccgtt atccacgccg gcaggtatct tcacctgaat gcgtttgctg cgcaccagtt
     1501 gcccctgtcc atcgcaattg gcgcagggtt tccccccgat ttcgccgagg ccgcgacaaa
     1561 tcgggcaaat ctcagtagat tgcacattga aggtgcgtgt cccgccagcg taggcttctt
     1621 gcagggtcac ttccaccggc tgatcgatgt tatcacccac gcgccgccgg atatcgtcgc
     1681 cggtgcgcgt cccacttgcc cgcgacccgc tgccgggcgt gttgccgaac agcgtctcga
     1741 agaaatcgga aaagttcgag gatgtatcaa agttgaatgg agacgagcgg aatccacccg
     1801 acgcgccaga agcgcgccga cccgtcccat agcccgcgct ggggccaaac tgatccatcc
     1861 agttcgggcc aagtgtatca tatttgcgac gtttttcggc atcggagaga acttcgtaag
     1921 cctcgttgat ctctttgaac ttctcctccg cttttttatc gcccgggttc acatccgggt
     1981 gatatttgcg cgccagtttg cgaaaggctt ttttgatctc ttccgcgctg gcgcttttcg
     2041 ccacgccgag gattttataa taatctttgt attccatctt ttgcccctct ttgcggcaaa
     2101 cgcttggagg ccatgctcgc taacgcgcgt ggcgcggcaa gacgcaaaac gtctggtgct
     2161 tacgcctgcg cttctttcct gcccgcgctc actttgacac gagccgggcg gaacgtatcg
     2221 ttccccagct tgtagccctt caagacttct tcgataactg tgccctccgg ctgattgcta
     2281 tcctccacgc tttcgatagc ctcgtgaaca cgcggatcaa acacctggcc aacagtagca
     2341 attggctcca gcccctcgtt gcgcagcacc tcgttcaggt gccacatcaa catccgcatg
     2401 gtttgcagca gttcctgccg ctccattttg tcctggtaga gcatagcgcg gtcgatatta
     2461 tccacgacct caaggacttt gttgagcaaa cgcgcctttt cctgctgcgc gcgctccatg
     2521 cccatcttca tctgtcgctt gcggaaatta tccatctcag cacgctcgcg caagtactta
     2581 ttccagttct ccgtcgcctc ctgccgtgcc tgtgccagtt gctgctccag cgccgcaata
     2641 cgctcgtcaa tcgaaatggg cgcgccggtc gtcgattcct ccgtcatccg ccctccctgc
     2701 atctcctgag aatcttgcat tgggtcttct atctccttta tgattgttta tgaaaaggat
     2761 ttatggtgct ttcagtataa atcttgagtg gggtgttgtc aagttttttg ggattaccag
     2821 ccctcactct ccaacggcgc atcttccctc cacttctccc cgcgctctag cgcgatcggc
     2881 gaaaagagca agtcttgcac gacagcgata cgagcatgct tccagagttc gaggactgcc
     2941 aggaaggtga cgatgatgat gacgcgcgag ggtgcttctt cgaggagttc gctgaggagg
     3001 acacgcgggc gggcgcgcag ccggtcaatg atttcagcga tacgctcgct gacgcgcaca
     3061 cgcgcggtag gaacgaaggc gatgccattc acctgctctc gcgcctgctg agccagcaaa
     3121 cgcgcaaagg cttgcgcgag tgtctctact tccaggccaa cgatggtagg tggcgtccag
     3181 gcgagctggg cttcgatacc ggccagcagg ccggagcgag cgaaggtttg caggccggcc
     3241 ttctcgcgca agcgcaggta acgcgcaatc tctttcgcca gcttgtattc gagcaggtgt
     3301 gcgcgcagtt cttccgccat agccgccgcg ctttccatct cgctcgtcat ttcctctttc
     3361 gtcgtgcgcg gcagcaggct ctgcgatttg atgaagagca ggcgagtggc gatggagaga
     3421 aaggcggcca tgtttgccag cggcatatta tcttcccgcc actggcgcag gtaagccata
     3481 tattgatcgg tcactgctac cagggagacg gtcgtaatct ccatctgccg cttctcgatc
     3541 aagtgcagga gcaggtcgag cggcccctcg aagatgggca ggcgcacaac atagttcgag
     3601 tgctcattat cagccagccc ttgctctcca gcaaccgccg tctgtagcag cccctcttgt
     3661 atttcttttt cgaccgaatc aagcattgct tatgaagcct cgtctaccag gtgtaattcg
     3721 gctgccgggc cgcctggttg gtgatgcgcg cgaatgtact gcacggcgtg ttccggcaga
     3781 ccggccccgg cagccatgtc cgcgccaacc tcggcgtgcc accaggcata accaagcgcg
     3841 cgacgccacc tgggcagccc atcgcgcggg tagactgcca cctgttgtaa taaacgcgga
     3901 gcaaagcgtt tgccgacgac gatgaccggg cgcgtccaga atggcactcg cccctgcgcc
     3961 ttgccaacat catgcaagag cgcggcagcc agcatgtccg gttcagcaca gccacgcgcc
     4021 tgcaacccct ggcaaacgcg cagcgcgtga cgctggtcgg cgggcgacat gctggtgaaa
     4081 agcgcgtagg ccgattccgg cagccagcgc gccacctctc gccgctcatc ctcggcgagc
     4141 ggcgagacga atcccagttg ctggctcacc tgccagagcc ggtaagcaaa tgctgccatg
     4201 gggttcaacc tatccatgta tcatataacg ggtggcccca ggtagatagc atacgcgcct
     4261 atacccccaa acccggtgat gagaccaatc agttgcatcg acccttgcag gatatagtaa
     4321 gagatgcgga acaggaaaaa atcgctcagc ccgacgagct ggcccaggaa cggcagcaag
     4381 aagatcagca gcaggatgat gaatggccca taggaggcac tcttcgcaaa gctgacggcc
     4441 tggcgactgg gcaacaggct gtagagtatc tgataaccat cgagcgggta gagtgggatc
     4501 aggttgaaga tcgccaggca aatgttgacg ctggcaaacg ttgcgacaag ctggggaatg
     4561 cgcacggcaa aggcattgag gtagagcgtg ccgggcagaa cacgcagcac gaccgcgaaa
     4621 atgccgccga tcagcaggct ggtgaagatg ccacccaggg ctacgatgag cgtgccgaca
     4681 tttggcccgc cgcgcaattt ccagggatcg gccttgaccg gattgcccca
//
LOCUS       DASZAK010000877         2139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_483867, whole genome shotgun sequence.
ACCESSION   DASZAK010000877 DASZAK010000000
VERSION     DASZAK010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483867"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..2013
                     /gene="thrS"
                     /locus_tag="VGT44_15400"
     CDS             223..2013
                     /gene="thrS"
                     /locus_tag="VGT44_15400"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV2582239.1"
                     /translation="MSVEAELEESIGYTPLQRMRHSAAHIMAEAVMEIFPDAKLAIGP
                     AIEDGFYYDFDLPRTLTPEDLPDIEQRMARIVAGKYPFIRDRWPREKALEYFREKGQT
                     YKVELIENLPDAEVGIYQQDTFLDLCRGPHVENTSQVGPFKLMKTAGAYWRGDENRPM
                     LQRIYGTAWFTQEELNQYLWRLEEAQKRDHRKLGRDLKLFIVSDEVPAGVPLFLPNGE
                     TLRHQMESYVRETQEKYGYQHVWTSHLGKVRLYKTSKHWYNYRESMFPVMQGEQEASE
                     DDAYVLKPMNCPHHITLYKSQMHSYRELPVRYAEFATLYRYEKAGTLTGLARVRSLTQ
                     DDAHVFMRPDQIQEEFNRAIDLTLEVFNTYGLKDYWIALSLRDPNHKEHYAGSDEVWE
                     KAESALREAVKNKGIEAREMIGEAAFYGPKTDFMVRDALGRDWQCSTVQLDFVQPENF
                     QLEYIGEDGQPHRPVMIHRAVTGSTERFMAMIIEHFAGAFPVWLAPVQAMIIPIADRH
                     QEYANKVLASLKSAGVRAEVDSRSERMNAKVRDAQLMKIPYMLVVGDKEASDNAVAVR
                     LRTNENLGATPLEVFVARITNIIKTKSRDL"
BASE COUNT          546 a          573 c          573 g          447 t
ORIGIN      
        1 taagaaatgg atacccctcg ccagcctgta ccgagcctgt aaggtacacg tcttgacctc
       61 gttaccggtc gctgaagcgc gtttccctga agtgtgacgc tcaatcacgt cagcaaatca
      121 ggtatgcgga agctgggtgg aaccacgaaa agtatcatag ccttttcgtc ccaaagaggg
      181 atggaaaggt ttttttattg ttggcaagag gagatttgaa tcatgtctgt tgaagcagaa
      241 ctggaagaat caatcggcta tacgccgcta cagcgcatgc ggcactccgc ggcacatatc
      301 atggcggagg cggtcatgga aattttccct gacgcaaaac tggctatcgg cccggccatt
      361 gaggacggtt tctattatga tttcgatttg ccacgcacgc tcacacccga agatttacca
      421 gacatcgagc agcgtatggc gcgtatcgtt gcgggcaaat acccatttat acgcgatcgt
      481 tggccccgcg aaaaggccct ggaatacttc cgcgagaaag gacaaaccta caaggttgaa
      541 ctgatcgaaa acctgcccga cgcggaagta ggcatctacc agcaggatac ctttctagac
      601 ctgtgtcgcg gaccgcacgt agagaatacg agtcaggtcg gtccattcaa actcatgaag
      661 acagcaggcg cctactggcg cggcgatgag aatcgtccca tgttacagcg catctatgga
      721 acggcgtggt tcacgcagga ggaactcaat caatacctgt ggcggctgga ggaagctcag
      781 aagcgcgatc accgcaagct ggggcgcgac ctcaagctgt ttattgtaag cgacgaggta
      841 cccgctggcg tgcccttgtt cttgccgaac ggcgagacgc tgcgtcacca gatggagagc
      901 tatgtccgtg agacgcaaga gaaatatggc taccagcacg tctggacatc gcatctcggc
      961 aaagtgcgcc tgtacaagac atccaagcac tggtacaact atcgcgagag catgtttcct
     1021 gtaatgcagg gcgagcaaga agcgtcggag gatgatgcct acgtgctcaa accaatgaac
     1081 tgcccgcacc acattacgct ctataagtcg caaatgcata gctatcgtga gttgcctgtg
     1141 cgctacgcag aattcgccac cctctatcgc tatgagaagg cgggaacatt aacgggcctg
     1201 gctcgcgtcc gcagtctcac gcaggatgat gcgcacgtct tcatgcgccc tgaccagatt
     1261 caagaggaat ttaaccgtgc tatcgacctg acgctggaag tgttcaacac gtacggactt
     1321 aaagactact ggattgcgct ctcgctgcgc gatccaaatc ataaagagca ttacgctggc
     1381 agcgacgaag tctgggagaa ggccgaaagc gcactgcgtg aagcggttaa aaacaaaggc
     1441 atcgaggcgc gtgaaatgat cggcgaagcg gctttctacg gtcccaaaac ggacttcatg
     1501 gtgcgtgatg ccctcgggcg tgattggcaa tgctcgaccg tgcagctaga ttttgtgcaa
     1561 cctgagaatt ttcagcttga gtacatcggc gaggacggcc aaccccatcg ccctgtcatg
     1621 atccaccgcg cagtaaccgg ctcgacagaa cgcttcatgg caatgatcat cgagcacttc
     1681 gccggggcat ttcctgtctg gctggcgccc gtgcaagcta tgattatacc cattgctgat
     1741 cgtcaccagg aatacgccaa caaagtcctt gcatcgctca aatccgccgg tgtgcgtgcc
     1801 gaggtagata gtcgcagtga gcgcatgaac gccaaagttc gtgacgcgca actgatgaag
     1861 atcccctata tgcttgtagt cggagacaaa gaagcgtcgg acaatgccgt cgctgtgcgc
     1921 cttcgcacca acgaaaacct cggcgcaaca cctctagaag tattcgtagc gcggatcacg
     1981 aatatcatca aaaccaaaag ccgagacctg taatccgtat tgtcggggcg aggtttcctc
     2041 tcgcccacaa gtaattctta ccatgtaatc tgtaggggcg gggtttatcc ccgcccgtgc
     2101 cgtcaacatg taacgctagt ccaataaaat aatacatac
//
LOCUS       DASZAK010000878         7315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_271649, whole genome shotgun sequence.
ACCESSION   DASZAK010000878 DASZAK010000000
VERSION     DASZAK010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271649"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VGT44_15405"
     CDS             <1..742
                     /locus_tag="VGT44_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582240.1"
                     /translation="SLMPSWYTFRHPQSRVLDRHGRYHDVSCYNQQPFRHWFYKEIEY
                     FLRTYPVNGIFLDEPRSVDVTCFCPVCRALCPDVADLERFRRRSLVDFLGEVCACVKR
                     IDSNIATTVALLPPDLPQVEDLAQLPALDTIACHLFWRSLSEDRDKVQTWSNAVAGAA
                     RQHRKRSQLWLQNFNLDEGEERELETDFSHVMAVEPDDLGCYYYWRNNANPTRVWEAT
                     RALLKRVPRRQLHWRVIKSGPKEETQAS"
     gene            739..1452
                     /locus_tag="VGT44_15410"
     CDS             739..1452
                     /locus_tag="VGT44_15410"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEV2582241.1"
                     /translation="MTSESTTSSVLLPPGGQRILVIMAHPDDAEFICGGTIARLTAER
                     CDVFYVLVTSGNRGSQEAGMTMERLAQIREEEQRRAAETLGVREVMFLGHNDGEVEVT
                     LALRSELAYAIRRFQPDVLFTFDPWRPYEIHPDHRNVGMCALDALAAARMPMYYPEQL
                     TGDMTQHRLKQVYLFSTHQPNHWVDISDTIEKKLEALHCHTSQVSASVDEIIRQRASL
                     AGVEHGYKYSESFHHMALR"
     gene            complement(1518..2309)
                     /locus_tag="VGT44_15415"
     CDS             complement(1518..2309)
                     /locus_tag="VGT44_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582242.1"
                     /translation="MGKLQEMFTQPLRAKSGSGLGFIGKNKAASKPRAAALVVEFATP
                     DAAIAEAAIKAGADGLLFAWNSKNAALDNIKKAIEAARSGEEKVVCGLDITSGWDTLT
                     RETFEHLKELEINYVILPLNAPARLLALQVKDLELVVSVPMRAGEMYPLFIRNLTAFE
                     GLASVRLEFGIQHDIGALSIEDILQFRAVREAVRFPSMLNIDSSISEADAYTLLTLGV
                     QALIIAANKTSDDTKQDVQLVHGLLETVYHEEKDSKDSFGLTPSR"
     gene            2591..3454
                     /locus_tag="VGT44_15420"
     CDS             2591..3454
                     /locus_tag="VGT44_15420"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV2582243.1"
                     /translation="MLQDGVDAYLTLLSNKKQGESNPNTIVAYRNDLQQLCAYLARFQ
                     LEDWQQVTREHISGYLLEMRDAQVYRPATIVRKLASLKTFFRYLRSSGVIATDPVEHL
                     GIPHIQKEPPQILSQEQVARLFRQVTTDSPVGKRDLAMITLLYTTGMRVSELVALNLD
                     DLDQRRSIITCAGRGGKSRHKRELPLLQPVVEMLRSYLQSARPHMIQHHPDEAALFVN
                     HHGERLTRQGFWLIVKGYARQAGITEITPQMLRHSFTALIPQKGAEARTLQEMLGLAH
                     LATARVVSRQS"
     gene            3616..4467
                     /locus_tag="VGT44_15425"
     CDS             3616..4467
                     /locus_tag="VGT44_15425"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV2582244.1"
                     /translation="MTLYDQVQEAAASIRARTVLQPAIAIILGSGLGDLAHDVQEAVA
                     LPYADIPHFQRSTVPGHAGRLLVGRLEGVPVVMMQGRFHFYEGYTLSALTLPVRVMHA
                     LGAQTLIVTNAAGGINPAYRPGDFMLIRDHIYLPGLVGANPLMGPNDERLGSRFPPVA
                     KAYDADLRNLASAVADGQPGATLHEGIYVMLSGPFFETGAELRFLRVIGADAVGMSTA
                     PEVVVARHEHMRVLGISLITNMATGDESEEVDHVGVLATADAARPRFAALVRGIVRGM
                     AAFPTQP"
     gene            complement(4454..5110)
                     /locus_tag="VGT44_15430"
     CDS             complement(4454..5110)
                     /locus_tag="VGT44_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582245.1"
                     /translation="MQTFTGRNPGGMSQQARRIATFAIILFGLSGLISGFAVGAFVHP
                     KLPGGTGGGGNGTPTTIARSTSTRTTTTPENVPVGEPLIKKFTYHEAGNGTKTYAFSV
                     EIVYDGTTTPIKESDVTCKLWLTADNGAMNNVLSANNNAILKNIDQISQPFAGEVQGA
                     FQFISPAQQVQNCVPGGGETTWNYTVSSSVDPGTYYIFSLADWQGKHYNWRSVQIKVV
                     "
     gene            5330..6559
                     /locus_tag="VGT44_15435"
     CDS             5330..6559
                     /locus_tag="VGT44_15435"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582246.1"
                     /translation="MQLPLTNHLPLASSSQSALTRRLLPLYIAAFSQGLVLWGPIEKV
                     FLKSIGFDQAALGLMAACYGSLIPLLDLTSGILADRWSRKGILMLASFASMLNALLGG
                     LSHDVPTYLVSTLFFGVEVAFVSGTYESIIYDTLLEHNGHSHAFGKCLGQLKLLNSLA
                     LIIGALIGGLVATLLSPRLAYFATLPLVAISLGTLLAFQEPHLHHADERISLKRHLRA
                     ITQILFQRGKTLPIVTVLLATSLLLSVIFEFGPLWQLALAAPVGLYGLANAAVLSAGG
                     VGSWLSTRLTLAKPATIVGVAGLLAGSSLALVVVRNAVVVILAQVILVAVGVGISMVF
                     TRLLHDSLSSQVRAGAASGVGALSSIAFVIFALIFGVVSQHADVFHAGWMVVGVALLA
                     SGLLVKVAVGQDFKTSR"
     gene            6643..7164
                     /locus_tag="VGT44_15440"
     CDS             6643..7164
                     /locus_tag="VGT44_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2582247.1"
                     /translation="MSIELVLLDSLRLPGKVKKSHMKHTNHRRSDCPINFALETFGDP
                     WSLLIIRDIVYFGKKTYGEFITSEEGMATNILASRLAHLEQQGILVKKPFPADKRKEE
                     YALTEKGLDLIPVLVEMANWSAEYDSGTAAPAAWIALMKADREKMIRLMRETVQNGGA
                     IFVGEKSLVGQLV"
BASE COUNT         1457 a         2162 c         2076 g         1620 t
ORIGIN      
        1 atcgctgatg ccgagctggt acaccttccg ccatccccaa tcgcgcgtct tggaccggca
       61 tgggcgctat cacgatgtct cctgttacaa tcagcagccg ttccgtcact ggttctacaa
      121 ggagatcgag tatttcctgc gcacctaccc cgtcaacggc atctttctcg acgagccgcg
      181 cagcgtggat gtgacgtgct tctgccccgt ctgccgcgca ctatgtcccg acgtggccga
      241 cctcgaacgc ttccgccgcc gctcgctggt cgatttcctc ggcgaggtgt gcgcctgtgt
      301 gaagcgcatc gatagcaata ttgccacaac ggttgcactg cttccgcctg atctccccca
      361 ggtcgaagac cttgcccagc ttcccgcgct ggataccatc gcttgccacc tcttctggcg
      421 ttcgctgagt gaagatagag acaaggtgca aacgtggagc aacgcggtgg ccggagccgc
      481 gcggcagcat cgtaagcgca gccagctctg gctacagaat ttcaacctgg atgaaggcga
      541 agaaagagag ttggaaaccg acttttccca tgtgatggcg gttgaaccgg acgacctggg
      601 atgttattat tactggcgaa ataatgccaa tcccacgcgc gtgtgggaag cgactcgcgc
      661 cctgctgaag cgtgtcccac gccggcaact gcactggcgc gtcatcaaat ctggcccaaa
      721 ggaagaaacg caggcatcat gacaagcgaa tcgacgacct cctctgtgct gctcccgccg
      781 ggcgggcagc gcatcctggt gataatggct catcccgacg acgccgagtt catctgcggc
      841 ggtaccatcg ctcgcctcac agcggagaga tgcgatgtct tttatgtgct ggtgacgagc
      901 gggaatcgcg gcagccagga agctggcatg acgatggagc gactggcgca gatacgcgag
      961 gaggagcagc gccgcgccgc cgaaacgctg ggcgtgcgcg aggtcatgtt tctgggccac
     1021 aatgacggcg aggtcgaggt cacgctggcg ctgcgcagcg aactcgccta tgccatccgc
     1081 cgcttccagc ccgatgtgct gtttaccttc gatccctggc gaccatacga gatccacccc
     1141 gatcatcgca acgtgggcat gtgcgccctc gacgccctgg cggccgcccg tatgcccatg
     1201 tactaccccg aacagttgac gggcgacatg acccagcacc gcttgaagca ggtttacctc
     1261 ttctcgacgc atcaaccgaa ccactgggtg gatatcagcg acaccatcga gaaaaagctc
     1321 gaagcgctcc actgccacac cagccaggtg agcgccagcg tggacgagat cattcgccag
     1381 cgcgccagcc ttgccggcgt cgaacacggc tacaaataca gcgagtcctt ccaccacatg
     1441 gcgttaaggt aaatacagaa caagcgaaaa accactccaa cccaccgggt tggagtggtt
     1501 tttcgcttgt tctgtattta ccgtgatggg gtcaggccga agctgtcttt cgagtccttc
     1561 tcctcgtggt agacggtttc cagcaggccg tggaccagtt gcacatcctg cttcgtgtcg
     1621 tcgctcgttt tgttggccgc gatgatgagc gcctggacgc ccagcgtgag gagcgtatag
     1681 gcgtcagctt cgctgatgga gctatcgatg ttcagcatcg aggggaagcg caccgcctcg
     1741 cgcaccgcgc ggaattggag gatatcctcg atcgagagcg cgccgatgtc gtgctggatg
     1801 ccaaattcca accgcacgga ggccagcccc tcgaaagctg tcaggttgcg gatgaacagc
     1861 gggtacatct cgcccgcgcg catcggcacc gagacgacca gctccaggtc tttgacctgt
     1921 agggcgagca ggcgcgccgg ggcgttgagc ggcaaaatga cgtagttgat ctccaactct
     1981 ttcaaatgct caaaggtctc gcgcgtcaat gtatcccacc cactggtgat atccaggccg
     2041 cagacgacct tttcttcccc actgcgcgcc gcttcaatcg ccttcttgat gttgtcgagc
     2101 gcggcattct tgctgttcca ggcgaagagc agaccgtcgg ccccggcctt gatggcggcc
     2161 tccgcaatcg cggcatcagg cgtggcaaac tcaaccacca gcgcagccgc gcgcggttta
     2221 gacgctgctt tgttcttgcc gatgaagccg agtccgctcc cacttttggc gcgcagcggc
     2281 tgtgtaaaca tttcttgtaa cttacccata ctctttcgta tgtctcctta ttaattcatg
     2341 gacacgatag aggtcgtgcc catgcctgta gttcttatgc gaggcggaac gtaattctgt
     2401 ggaactgcgt tgttgggact attatatcac gtttcgggat acctgtactt cctattttat
     2461 gcgttattaa ttgtctaatt gcccccatat acagtataat ggcggagtca tgtttcacaa
     2521 aatattcaag ggaacgaaag aagcgaggtg acctcctcgt gaagataaag aagctagatg
     2581 gaagtgctgt atgttgcaag atggtgttga tgcatacctg acattgctct cgaacaaaaa
     2641 gcaaggcgag agcaacccca atacgattgt agcttaccgg aatgatctcc agcagttgtg
     2701 cgcttacctc gcccgttttc aattggaaga ttggcagcag gtcacacgcg agcatatcag
     2761 cgggtattta ctggagatgc gcgacgccca ggtctatcgc cccgcaacga ttgtgcgcaa
     2821 gctggcctcg ctgaaaacct tctttcgcta cctgcgatcc agcggcgtga tcgctaccga
     2881 cccggtcgaa cacctgggta ttccgcacat tcaaaaggag ccgccgcaaa tcttgagcca
     2941 ggaacaggtc gcccgcctct ttcgccaggt gactaccgat agccccgtag gcaaacgcga
     3001 cctggccatg ataacgctgt tgtatacaac tgggatgcgt gtcagcgagc tggtcgcgtt
     3061 gaacctggac gacctggatc agaggaggtc gatcattacg tgcgcgggac gcggtggcaa
     3121 aagccgccat aaacgcgaac tgcccctgtt gcaaccggtg gtagagatgc tgcgttccta
     3181 tctccaatcg gcgcgcccgc acatgatcca gcatcacccc gacgaagccg ccctgttcgt
     3241 caaccatcac ggcgagcgac tgacgcgcca gggcttctgg ctgatcgtca aaggctacgc
     3301 gcgccaggcg ggcatcaccg aaattacgcc acagatgctg cgtcattcct tcaccgcgct
     3361 catacctcag aaaggcgctg aggcgcgcac cctccaggaa atgctcggcc tcgcccatct
     3421 cgcgaccgcg cgggtggtaa gcaggcaatc ctaatgcatg gcgaccgcaa gggtacgcca
     3481 ctacgataac gctcggggtt caccgcctcg tcgttatcgt agtggcgtac ccttgcggtc
     3541 gccatgcctc acgtagcttt gtcaagtcga gggtataata tctgtacagt ccgatagcag
     3601 aagaggaaaa caggtatgac actctacgat caggtgcagg aagccgctgc ctctattcgc
     3661 gcgcgcaccg tgttacagcc cgccatcgcc atcatcctcg gctccggcct gggcgacctg
     3721 gcgcatgacg tgcaggaggc tgtcgcgctg ccctacgcgg atatccccca tttccagcgc
     3781 tccaccgtgc ctggtcacgc gggccgtttg ctggttggca ggttggaagg cgttccggtg
     3841 gttatgatgc aggggcgctt ccacttctac gagggctaca ccctatccgc gctgacgctg
     3901 ccggtgcgtg tcatgcacgc gctgggcgcg caaacgctga ttgtcaccaa cgcggcgggc
     3961 ggcatcaacc cggcctatcg acccggcgat ttcatgctga ttcgcgatca catctacctg
     4021 cccggcctgg tgggcgcaaa ccccctgatg ggaccgaacg atgaacgcct ggggtcgcgc
     4081 ttcccacccg tcgccaaagc ctacgatgcc gatctgcgca acctggcaag cgcggtggcc
     4141 gatgggcagc ccggcgcaac gttgcacgaa ggcatctacg tcatgctgag cggccctttc
     4201 ttcgagaccg gcgcggagct gcgcttcctg cgcgtgattg gggccgacgc cgtgggcatg
     4261 tctaccgcgc cggaggtcgt cgtggcgcgg cacgagcata tgcgcgtcct ggggatcagc
     4321 ctcatcacca acatggccac cggcgacgaa agcgaggagg tcgatcacgt cggcgtcctg
     4381 gccaccgccg acgccgcccg cccgcgcttc gcagccctgg tgcgcggcat cgtgcgcggg
     4441 atggctgcct tccctacaca accttgatct gtaccgaacg ccagttgtag tgcttcccct
     4501 gccaatcggc cagcgagaat atatagtatg ttccggggtc taccgacgag gataccgtat
     4561 agttccaggt ggtttcccct ccccctggta cgcaattctg tacctgctga gccggcgaaa
     4621 tgaattggaa cgcgccctgg acttcacccg caaatggctg gctgatctga tcaatatttt
     4681 tcaatatcgc gttattattt gcgctcaata cattattcat agcgccgtta tccgctgtga
     4741 gccagagttt gcaggtcaca tccgattctt taattggtgt cgttgttcca tcatagacga
     4801 tctcaaccga aaaggcatac gttttagtcc cgttcccagc ctcgtgatag gtaaatttct
     4861 tgataagcgg ttctcccacc ggcacattct caggggtggt cgtcgttcgt gtcgaggtac
     4921 tccgcgcaat cgtcgtcggc gtcccatttc cccctccgcc cgtgcctccc ggcagcttcg
     4981 ggtgtacgaa cgcgccgacc gcaaagccgc taatcagccc ggagaggccg aacaggatga
     5041 tggcgaacgt agcaatacgc cgcgcttgct ggctcatgcc gcctgggttg cgtccggtga
     5101 atgtttgcat tgtattggct gtctctctag ttattcattg gtcttttgtg attgtgtatt
     5161 acgtttcatc atagcacaag gcttcacctg ttttgttgaa atgaacggga gatagcccga
     5221 tttggtagaa tttagttacc gcttttcctt ctcggtttga gaataggata gtagatttac
     5281 ttgcgatttg caagtgacac tacttttccg agagaaggag aacgaagaca tgcagcttcc
     5341 cctcacgaac catctgcctc tcgcgtcatc gtctcagtcg gcgctcacca ggcgcctgct
     5401 acctctctac atcgcagctt tttctcaggg gttggtactt tggggcccca ttgagaaggt
     5461 gtttctgaaa agtattgggt ttgaccaggc tgcccttggt ctgatggccg cctgttatgg
     5521 cagtctcatc ccgctgctgg atctgacctc gggcatcctg gccgatcgct ggagtcgcaa
     5581 gggcatcctc atgctggctt cattcgcttc catgctcaac gcactgctcg ggggactcag
     5641 tcacgatgtg ccgacctacc tggtcagcac tctcttcttc ggggtcgaag tcgccttcgt
     5701 ttcgggcacc tacgaatcca tcatctacga cacgctgctt gagcacaacg gacacagtca
     5761 cgcctttggg aagtgtctgg gccagctgaa gctgctcaat agtctggcct taatcatcgg
     5821 cgcgctcatc ggcgggctgg tagccacgct gctttctcct cggctggcgt actttgccac
     5881 gctcccgttg gtggccattt ctctgggcac cctgctcgcc ttccaggagc ctcacctgca
     5941 tcatgctgac gagcgcatct ctctgaaacg ccatctgcgg gccatcactc aaatcctctt
     6001 tcagcgaggg aaaaccctgc ccatcgtcac cgttttactg gccacctcgc ttttactctc
     6061 ggtgatcttc gagtttggcc cgctgtggca gctggccctg gccgccccgg tcggacttta
     6121 tggcctggcc aatgcggccg tactctcggc gggaggggta ggctcatggc tttcaacccg
     6181 tctgacgctt gcgaaaccgg ccaccatcgt gggggttgcg gggctgctgg cgggatcgag
     6241 cctggcgctg gtcgtcgtga gaaatgctgt ggtcgttatc ctggcccagg tgattctggt
     6301 cgcagtggga gtggggatca gcatggtctt tacccgcttg ctgcacgact ccctctcctc
     6361 acaggtgcga gcgggagcgg catccggggt gggcgccctc tcctcaatcg cctttgtgat
     6421 cttcgcgctg atctttggag tggtgagcca gcacgccgac gtttttcacg ccgggtggat
     6481 ggtagttggg gtggcgctgc ttgccagcgg gctgctggtc aaagtggctg taggccagga
     6541 cttcaaaaca tctcggtaac gctctttgtt cgccctatta cttgcaattt acaagtgaaa
     6601 aatgctatac tataccagaa ccaggaagga gatttggaat gagtgagtat cgagctggtc
     6661 ttgctggaca gcttgcgcct tcccgggaaa gtgaagaagt cgcacatgaa gcatacaaac
     6721 cacaggcgct cggattgccc catcaacttt gccttagaga cgtttggcga tccgtggtcg
     6781 ctgttgatca tccgtgatat cgtctacttc ggcaagaaaa cgtatgggga atttatcaca
     6841 tcagaagaag ggatggcaac caatattctc gccagccgtc tcgctcatct ggaacaacag
     6901 ggcattctgg tgaaaaagcc ctttcctgca gataaacgaa aagaggagta tgccctgaca
     6961 gaaaaagggc ttgatctcat cccggtcctg gtggaaatgg cgaactggag cgccgagtat
     7021 gattcaggca ccgctgcccc tgccgcctgg attgccctga tgaaggccga ccgagagaag
     7081 atgatccgtc tcatgcggga aacggtgcag aacggtggcg ccatttttgt cggcgagaag
     7141 agtcttgtgg gtcaactcgt ctaaaaaaaa gagaccggtc attgtcctgg gggaaacatg
     7201 accggtgtac ccagataagg ggtgtgtggg ggaagtcctg ggtccagaat accgccagcg
     7261 cttccggcaa ggatggctca atggagctga tgaccaggta tgccacgaga cattg
//
LOCUS       DASZAK010000879         4479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_520908, whole genome shotgun sequence.
ACCESSION   DASZAK010000879 DASZAK010000000
VERSION     DASZAK010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520908"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..1857)
                     /locus_tag="VGT44_15445"
     CDS             complement(325..1857)
                     /locus_tag="VGT44_15445"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEV2582248.1"
                     /translation="MANAGPSDGDIRATLALSQVEARAGSSRTLNLPGGRTVTIDIPA
                     GTRNGEELRFPGQGDELWQGGPVGDLVLTVSIPPGNPMSNPSNPGFYQNAPGSPNLPT
                     ELGSAPSFPSPGYNPSAGMPSTSYSPPSGYSPPPAGQTMPAYASSPDYYSGQPAQQPQ
                     EPLFLPQDKTAYADPGAYQPYSVPAQPPAYQPYAPPGQQAGYVQQPPAAPSPPRKRQG
                     RTMTSAIIGLIFVLILLLLIGSGLIYYFGYYQPNQLHAQATATANAQASATAIAITQA
                     TGTAIAQASATANAQASATANAQATANAQASATATALQGVLTQATSGTPALNDPLNGQ
                     SNNNGWDILSPSQSTVSGSCSFTNGAYHSMMPTKGYFQPCYARNATFANFAFQVQMTI
                     VQGDQGGLLFRADPNNSKFYLFRINSTGTYDLFLYIDNNGNDAKDLVSGTAPAFKTGT
                     NAQNTITIVAKGGTIYVYINGKYTDSVSNNTFASGLIGVFGASNGTSTDVAFSNAEVW
                     QI"
     gene            complement(1896..2204)
                     /locus_tag="VGT44_15450"
     CDS             complement(1896..2204)
                     /locus_tag="VGT44_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HEV2582249.1"
                     /translation="MAVVNEAEVMDALHECYDPEIPVNIVDLGLVYNVDIDDSGKVEI
                     TMTLTSLGCPLAGEVIDEIQTRIGEVENVTSCKVNMTFDPPWSPERMTEDAKWELGMI
                     "
     gene            complement(2361..3323)
                     /locus_tag="VGT44_15455"
     CDS             complement(2361..3323)
                     /locus_tag="VGT44_15455"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEV2582250.1"
                     /translation="MQRIILDTDPGIDDALALFLALASPDIQLEAVTTVSGNVHVDLT
                     TRNALTLLELAGRDDIPVARGSDRPLVRQPVIADYVHGRNGLGDAILPEPRLQPVAAH
                     AVDLMIEKIMAAPGEITLVPIGPLTNIALAVRRQPRIAQRVREVVIMGGALRVPGNVT
                     PASEFNIYADPHAARIVLQAGWPVRLVSLDVTNQAKMQREQLHVLAANAHPVTQLMQQ
                     MVSYSIETFSKPRGLSAFSMHDPLCLAAALRPDLIDWKPAYVDVELAGTLTLGATVAY
                     FERLEDIDPALEYQHRANLQASTGVDVEQFLDFYMERILATFHS"
     gene            complement(3380..4327)
                     /gene="rbsK"
                     /locus_tag="VGT44_15460"
     CDS             complement(3380..4327)
                     /gene="rbsK"
                     /locus_tag="VGT44_15460"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEV2582251.1"
                     /translation="MSRRTILTVGSLNMDQVVQVPHIPTLGETLLGAGSLRLVPGGKG
                     ANQAVAMARLGASVAMAGRVGSDPFGERLLAALQADGVDTRLVEVDGEEASGTAFIFL
                     EPGGDNAIVVASGANAQVGADVRQMARIRAAIPGAAALVLQLEIPIATVKNLIAAGHA
                     ARVPVVLNLAPAQPLSWETLSQLAVLIVNETEASMLSGQRVETLDDARTVATVLHEHG
                     ISSVIITLGAQGALLATSDRAGKLECFHQPAPAVQVIDTTAGGDCFAGAYTVALSEGQ
                     PPRDALRFAVYASALKVTKFGAQSGLPARAEVEAFLKRT"
BASE COUNT          833 a         1240 c         1432 g          974 t
ORIGIN      
        1 gcgcccctac acatctttcg gcggttgacg gaccataaaa aaacgccatc atcctttggg
       61 aaggacgacg gcgcggattt cacgcggagg tcgtggtgcc accttgattc gctcttcctt
      121 tgctgcgaaa gagcctcgaa atgcccggta acgggggctg ccgttggcgc ttctcgcgct
      181 ggctcgcggg tggatgtgtt ccctcgcccg gctgctgcgt tgcaccggac cgcagctcgc
      241 tgttgccctt agagggtact tgtcccgttc atggcctgtt gttgtgagcg tagtgtagca
      301 gtgtatgtaa tggatgtcaa tagtttagat ttgccagact tccgcgttgc tgaaggcgac
      361 atcggttgaa gtgccgttgc ttgcgccgaa gacgccgatc agaccggaag cgaaggtatt
      421 gttgctcacg ctatccgtgt acttgccgtt gatgtaaacg tagatcgtgc cgcctttcgc
      481 cacgatggtg atggtatttt gcgcgttggt gccggtctta aaggccggcg cggtgccgct
      541 taccaggtct ttggcgtcgt tcccgttgtt gtcaatatag agaaacagat cgtaggtgcc
      601 agttgaattg atgcggaaca agtagaattt tgaattgttg ggatcggcgc ggaagagcag
      661 tccgccctgg tcgccctgga cgatagtcat ctgcacctgg aaggcgaagt tagcgaacgt
      721 cgcattcctg gcatagcaag gttggaagta gcctttcgtc ggcatcatgg aatggtatgc
      781 gccgttggta aaggagcaac tgccgctcac cgtagattgt gaaggcgaga gaatatccca
      841 gccgttgttg ttgctttgcc cgttaagcgg gtcattcaac gcgggtgtgc cgctggtcgc
      901 ctgcgtcagg acgccttgca gggcggtcgc tgtggcggat gcctgggcgt tagcggtggc
      961 ttgcgcgttg gcggtggcgg acgcctgggc attggcggtg gcggacgcct gggcgatggc
     1021 ggttccggtg gcctgagtaa tcgcaatcgc cgtcgcggac gcctgggcgt tggccgtcgc
     1081 ggtggcctgg gcgtgcaatt gattgggctg gtagtagcca aagtagtaga tcaggccgct
     1141 cccaatcagc agcagcaaga tcaaaacgaa gatcagccca ataatggcgg aggtcatggt
     1201 ccgaccctga cgcttgcgtg gtggtgacgg agctgctggg ggctgctgca cataacctgc
     1261 ttgctggcca ggcggggcat atggctggta tgcgggcggt tgagcaggca cgctgtaggg
     1321 ctggtatgcg ccaggatcgg cataggccgt tttgtcctgt ggcaggaaga gcggctcctg
     1381 cggctgttgc gctggctggc cggagtagta gtctggagat gatgcatatg caggcatcgt
     1441 ctgtcccgca ggcggcggcg agtaaccgga aggaggcgaa tagctggttg aaggcatacc
     1501 tgcgctcggg ttgtagccgg gtgacgggaa cgacggcgct gaacccagct cggttggtag
     1561 atttggcgag ccgggtgcgt tttgatagaa acctggattc gacgggttgg acattggatt
     1621 gccggggggg atcgaaaccg taagtaccag gtcgccaacc gggccgcctt gccagagttc
     1681 gtcgccctgg cctgggaaac gcagttcttc tccgttccgg gtaccggcgg gaatgtcgat
     1741 cgtgaccgtt cgccctcctg gaaggttgag tgtgcggctg cttcccgccc tggcttctac
     1801 ctggctgagc gcgagcgtgg cgcgaatgtc tccatcggat ggacctgcat ttgccataac
     1861 aacacctacc tgagcttata aactattcga ccgtatcaga tcatgcccag ttcccattta
     1921 gcatcctctg tcatgcgctc tggcgaccag ggcgggtcaa aggtcatatt cactttgcac
     1981 gacgtgacgt tttcgacttc gccaatgcgc gtctggattt cgtcgatgac ctcgccggcc
     2041 agcggacaac ccagcgatgt gagggtcatg gtaatttcga ctttaccgct atcatctata
     2101 tcaacgttgt agaccagccc caggtcaaca atattgacgg ggatttccgg gtcgtaacat
     2161 tcatgcagcg cgtccatcac ttctgcttcg ttcacgactg ccatgatttt cctgctcctt
     2221 tgtgcttaaa ccgagtactt ccttgcatcc tgctcgaccg caggtaggac gcagtctctt
     2281 agcaggatgg ctttcaacgt agattcataa ggattaattt atcctaatga tagcttatca
     2341 cactgcgctt gtcaaaggcg ttatgaatga aatgttgcca gaatccgttc catgtaaaag
     2401 tcgagaaatt gctcgacatc gacgcctgtg gacgcctgga ggttcgcgcg atgctggtat
     2461 tcaagggcag gatcgatatc ttcgagccgc tcaaaatagg cgacggttgc gccgagcgtg
     2521 agcgttccgg ccagttccac atcgacatag gcgggtttcc agtcgatgag atctgggcgc
     2581 agcgccgccg ccaggcagag cgggtcgtgc atactgaagg cggagaggcc gcgtggtttg
     2641 ctgaatgtct caatagagta ggaaaccatt tgctgcataa gttgtgtcac aggatgtgcg
     2701 ttggcagcca gcacatgcaa ctgctcgcgc tgcatcttcg cctggttggt gacatccagc
     2761 gagacgagac gcacaggcca gcctgcttgc agcacgatgc gcgcggcgtg tggatcggcg
     2821 taaatgttga attcactggc gggtgtgacg ttgccgggaa cgcgcagcgc gccgcccatg
     2881 atgacgacct cgcgcacacg ctgcgcaatg cgcggctggc gtcgcactgc cagggcaatg
     2941 ttggtcaatg ggccgatggg gacaagcgta atctcgcccg gagcagccat gatcttctcg
     3001 atcatgagat cgacggcgtg tgcggcgact ggttgcagac gcggttccgg caggatcgcg
     3061 tcgcccaggc cgttacgccc gtgtacgtaa tcggcgatga ctggctggcg caccagcgga
     3121 cggtcgctgc cgcgcgcgac ggggatatcg tcacgtcccg ccagttccag cagcgtgagg
     3181 gcattgcgcg tcgtcagatc gacgtggacg ttgccgctca ctgtcgtgac cgcttccaac
     3241 tgtatgtccg gtgaggccag ggccagaaag agcgccaggg catcgtcgat gccgggatcg
     3301 gtatctagaa tgatgcgttg catggtgtta aagagagcct ttcatctctg ccgttcgggc
     3361 aatccccgac cgcctggcgt tatgttcgtt tgagaaatgc ttccacctcg gcgcgcgccg
     3421 gtaagccgga ctgcgcgcca aatttggtga ccttcagcgc gctggcatag acggcgaagc
     3481 gcagcgcgtc ccgcggcggc tgtccctcgc tcaaggcgac ggtgtatgcg cccgcgaaac
     3541 agtcgccgcc tgctgttgta tcgatcacct ggacggcggg cgctggctga tggaagcact
     3601 ctagcttgcc cgcgcggtcg gatgtcgcca gcagcgcgcc ttgcgctcca agcgtgatga
     3661 taacgctgga gatgccatgt tcgtgcagca ccgttgccac cgtgcgcgca tcgtccaatg
     3721 tttcgacgcg ctgcccgctc agcatactcg cctcggtttc attcacaatc aggaccgcaa
     3781 gttgagagag cgtctcccag gagagtggtt gcgcgggcgc gaggttcaac actaccggta
     3841 cgcgcgccgc gtgaccggcg gcaatcaggt ttttgacggt tgcgatgggt atttccagtt
     3901 gcagtacgag cgcggctgcg ccgggtatgg ctgcgcggat gcgggccatc tgccgcacat
     3961 ccgctccaac ctgcgcatta gccccggatg caaccacgat ggcgttatct ccccccggct
     4021 ccagaaagat gaaggcggtg ccactggctt cctcgccatc gacctcgaca aggcgcgtat
     4081 ctactccgtc ggcctgtaga gccgcgagca ggcgctcgcc aaagggatcg ctgccgaccc
     4141 gaccggccat ggcgacgctg gcgcccaggc gcgccatggc gacggcctgg ttcgcgccct
     4201 tgccccccgg caccagccgg agcgaacccg cgcccagcag cgtttcaccc agcgtaggga
     4261 tgtgtggcac ctgcaccacc tggtccatat tcaaacttcc gacggtgagg atcgtgcgtc
     4321 gggacatcgc ctgtgcctcc tttcgcgccc ctggaatggt gttttgttga gtataaccga
     4381 aaattggaaa aggatacaag tgatgaaaat gagcgcagcc tcactagtcg attcgggaca
     4441 aacgcccagg aggatcggga caaagtggaa gcaagcgac
//
LOCUS       DASZAK010000880         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_520913, whole genome shotgun sequence.
ACCESSION   DASZAK010000880 DASZAK010000000
VERSION     DASZAK010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520913"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1197
                     /locus_tag="VGT44_15465"
     CDS             <1..1197
                     /locus_tag="VGT44_15465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907707.1"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEV2582252.1"
                     /translation="QGYIKHIEEDSSQAAPEAQPVEGTGDVEVRTTTGQSTQEMLLNL
                     GPQHPSTHGVLRLLLTLEGETVVDCTPVIGYLHRGIEKTLENRPYMGGIRYTDNSDYL
                     SPMLNEIAYAGAVEQLMGLEPPRRAQYIRLLVGELQRIASHLVGIGAYVNDLGATTPL
                     LWSMRDREGVMDLFEALGGSRFNVNYVRVGGVLHDFPSGWLKSCTIYLDQLEKHMGEL
                     DRLINGNEIFLARTQGVGYLDPQQAIAYGITGPMARASGIGWDLRVNSPSMAYREVPV
                     QVQVRQEGDCYARNAVRMQEIQESIRLCRVAIDKLPAGPIGTRTPVALRPPRGETYFA
                     IEGSKGELGVYLISDGSEYPWRAKLRGPSFVNLQILPELVRGYKISDVIAVLGSLDFV
                     VGEVDR"
     gene            complement(1236..>1504)
                     /locus_tag="VGT44_15470"
     CDS             complement(1236..>1504)
                     /locus_tag="VGT44_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582253.1"
                     /translation="NSVGQEQLVGHLPELARIAAEDSSDWQDRLLIGLACRSALRRGR
                     ELGINEQRSLITALASASAPAVCPHGSPILLHYSRTFLIDKFDW"
BASE COUNT          335 a          424 c          448 g          297 t
ORIGIN      
        1 caaggataca tcaagcatat tgaagaggac tcttcacaag cggctcctga agcgcagcct
       61 gtggagggaa ccggtgacgt ggaagtgcgg accacaacag gtcagtccac acaagagatg
      121 ctgctgaatc tggggcccca gcaccccagc acccatggcg ttctgcgcct gctgctgacc
      181 ctggaagggg agacggtggt ggactgcact ccggtcattg gttacctgca tcgcggcatc
      241 gagaaaacgc tggagaatcg tccttacatg ggcggcatcc gctacacgga caattccgac
      301 tacctctccc cgatgctcaa tgaaatcgcc tatgccggag ccgtcgagca gttgatgggg
      361 cttgagccgc cgcgacgagc tcagtatatc cgcctgctcg tgggggaact gcagcgcatc
      421 gccagtcacc tggttgggat tggcgcctac gtgaatgatc tgggagcgac aacacccctc
      481 ctgtggtcca tgcgcgaccg cgaaggagtg atggatctct tcgaggcgct gggcggcagt
      541 cgtttcaatg tcaactatgt gcgcgtcggt ggcgtcttgc acgacttccc cagcggctgg
      601 ctcaagtcat gcacgatcta cctggatcaa ttggaaaaac acatgggcga actggaccgg
      661 ctcatcaacg gcaatgaaat cttcctggcg cgcacgcagg gcgtaggcta tctcgatccg
      721 cagcaggcca tcgcttatgg tatcaccggc cccatggcgc gtgccagcgg catcggctgg
      781 gacctgcgtg tcaatagtcc ctctatggca taccgcgaag tgccagtgca ggtccaggtg
      841 cgacaggaag gcgattgcta cgcacgcaac gccgtgcgca tgcaagaaat tcaggagagt
      901 attcgcctct gccgtgtagc gatcgataaa ttgcccgcgg gccctatcgg tacacgcaca
      961 ccagtggcct tgcgcccacc acgcggcgaa acctactttg ctattgaggg cagcaagggc
     1021 gaattgggcg tataccttat cagcgatggc agcgagtatc cctggcgcgc gaagctgcgc
     1081 ggaccatctt ttgtgaacct gcaaatcctg ccggagttgg tgcgcggcta taagataagc
     1141 gatgtcattg cggtgctggg cagcctggat ttcgtggtgg gagaagtgga tcgctaggcc
     1201 gtcacaaaac atgatcatga gcaaaacggt agtagtcacc aatcgaactt atcaatcagg
     1261 aatgtcctgc tgtagtgcag gagaatcggg cttccatgag gacagacggc tggggcagag
     1321 gcgctcgcca gtgctgtgat taaagagcgc tgctcattta tacctaactc acgcccacga
     1381 cggagagcag aacgacaggc caggccaatc aagaggcgat cttgccaatc gctgctatcc
     1441 tcagccgcta tgcgtgcaag ttcgggtaaa tgccccacca gttgttcctg tccaacacta
     1501 ttgg
//
LOCUS       DASZAK010000881         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_483946, whole genome shotgun sequence.
ACCESSION   DASZAK010000881 DASZAK010000000
VERSION     DASZAK010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483946"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2385)
                     /locus_tag="VGT44_15475"
     CDS             complement(<1..2385)
                     /locus_tag="VGT44_15475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582254.1"
                     /translation="MFRSKAKTYAALFGLFLSLVLITTVVLRTFAEPVQSYYNHFLST
                     HYNDDGQYDDNAPPGTLSANLAQEQNSDQAYPNAYVGYDQVMQAYNAYLALSRQPSAA
                     VGSTWRLLGPVTGNVSSLVTYTGRATTASGRVTALVVSPDCNDTLCRVWVGAAGGGVW
                     ETDNGLSPVPIWQSSSSGLASNAIGSITLDPNDATGATLYAGTGEENGSSDSEAGVGL
                     YKSTDYGQTWHLVPGSVAVAKDRAIGSIAIDPSNPQHILIGTDVARHGASSVNGGRMT
                     PPNAPQIGLYESTDGGASFTLAFSKPSDSVDPTSPNGSDFFRGGVTNIVFNNTGLASG
                     WRVYFSMFDYGLFRSTGSGTYEQVFASVGGGTVANSAGARTEFALAPDGSKLRIYLGD
                     TDGNTADFYRVDDANVPAPTLTDGTNNPGWTKLSNATKGTPGYASYNFCAPQCSYDMP
                     IASPPGQPNNVWIGGSMQYNEIFTANPPSNGRAVQRSTDAGVDFTDMTNDAQTPPMGM
                     HPDQHVIAFDPANPDIAFVGSDGGLVRMSGAFSDTSSSCATRGLTGADLTDCQSWLKA
                     VPTQIFSLNDGLATIQFQSITVNPLHPKTDVIGGSQDNGTWAYTENPKNWIETVGGDG
                     GQSLIDYVNPNIRMHTYYGPSIDVNFNSDDPAGWNYISDPLTASKEASSFYVPLVADP
                     QVSGTMFVGLEHIWRTVDSGGPQAYLLQHCNEQKGDFQQPCGDWQALGTATLTSTTYG
                     TDRAGGYVAAISRAPGAENVLWTATRFGRLFISTNADAPAARVTFTRLDTAAQPG"
BASE COUNT          479 a          729 c          760 g          468 t
ORIGIN      
        1 cccgggctgc gccgccgtgt ccagccgcgt gaaggtcacg cgtgcggcag gcgcgtccgc
       61 gttcgtcgag atgaagagcc gaccaaagcg cgtcgccgtc cacaacacgt tctcagcgcc
      121 cggcgcgcgg ctgatcgccg ccacataacc gccggcgcgg tcggtgccat aggtcgtact
      181 ggtcagagtt gccgttccca gcgcctgcca gtcgccgcag ggctgctgga aatcgccctt
      241 ctgctcgttg caatgctgca gcaggtacgc ctgcgggccg ccgctatcga cggtgcgcca
      301 gatatgctcc aggccaacga acatcgtgcc gctcacctgc ggatcggcaa ccagtggtac
      361 atagaacgag gacgcctctt tactggcggt cagcgggtcg ctgatgtagt tccagcccgc
      421 cgggtcatcg ctgttgaagt tgacatcgat gctggggccg tagtaggtgt gcatcctgat
      481 gttgggattg acgtaatcga tcagcgattg gccgccatcg cctcccaccg tctcaatcca
      541 gttcttgggg ttctcggtgt aggcccacgt gccgttatcc tgcgagccgc cgatcacgtc
      601 tgttttggga tgcagcggat tgacggtaat gctctggaac tggatcgtcg ccaggccatc
      661 gttcaagctg aatatctgcg tcggaacagc cttcagccag ctctggcagt cggtcaggtc
      721 ggcgcccgtc aggccgcgcg tggcgcagct gctcgatgtg tcgctgaacg cgccgctcat
      781 gcgcaccagg ccgccatcgg agccgacgaa ggcgatatca ggattagctg gatcgaaggc
      841 gatcacgtgc tgatcgggat gcatccccat cggcggtgtc tgcgcatcgt tggtcatatc
      901 cgtgaaatcg acaccggcat ccgtggaacg ctgcacagct cgtccgttcg agggcgggtt
      961 ggccgtgaag atttcgttat actgcatcga gccgccgatc cacacgttgt tgggctgacc
     1021 tggaggcgag gcgatcggca tgtcgtagct gcactgcggg gcgcagaagt tatacgaagc
     1081 ataaccgggc gtgcccttcg tcgcattcga cagctttgtc cagccgggat tgttcgtgcc
     1141 atcggtcagg gtcggtgctg gtacgttggc atcatccacg cggtagaaat cagccgtatt
     1201 gccatcggtg tctcccaggt agatgcgcag tttgctgcca tcgggggcca gtgcaaactc
     1261 ggtgcgcgcg cccgcgctat tcgccaccgt gccgccgccc accgaggcga agacttgctc
     1321 atacgtgccg cttccggtgc tacggaacag cccatagtcg aacatcgaga aatagacacg
     1381 ccagcccgac gccagcccgg tattgttaaa gacaatattg gtcacaccgc cccggaagaa
     1441 gtcgctgccg ttgggcgagg tcgggtcaac actgtcggat ggcttgctga aggccagtgt
     1501 gaaggacgcg ccgccatcag tcgactcgta cagcccaatc tgcggcgcgt tgggcggcgt
     1561 catgcgcccg ccgttgacgg atgacgcgcc gtgtcgcgcc acgtcggtgc cgatcaggat
     1621 gtgctgcggg ttggacgggt caatggcaat cgaaccgata gcgcggtctt tagcgacagc
     1681 cacgctgccg gggacaaggt gccaggtctg accgtaatcg gtggacttgt agaggccgac
     1741 gcccgcctcg gagtcgctgg aaccgttttc ttcgccggtg ccggcataca gcgtcgcgcc
     1801 ggtcgcatca ttggggtcaa gcgtaatcga accgatcgcg ttcgacgcca gcccgctcga
     1861 agacgactgc cagatcggaa ccggcgaaag accattatct gtctcccaca cgccaccgcc
     1921 agccgcgccg acccacacac ggcacaacgt atcattgcag tcgggcgaga cgaccagcgc
     1981 cgtgacgcgc ccggaagcgg tcgtcgcgcg gccggtatac gtcaccagcg aagagacatt
     2041 gccggtcacc ggaccaagca ggcgccaggt tgagccgacc gcggcggaag gctggcgcga
     2101 aagggcaaga taggcattgt aggcttgcat cacctggtca tagccaacat aggcattggg
     2161 ataggcttga tcgctatttt gctcctgcgc aaggttggca gaaagcgttc caggaggggc
     2221 gttatcatca tactgtccat catcgttata atgcgtactc aaaaagtggt tgtagtacga
     2281 ttgcaccggc tcggcaaagg tgcgcaggac aacggtagtg atgagaacaa gacttagaaa
     2341 gagaccgaac agagcagcat aagtttttgc tttagaacga aacatacgaa gggggacctc
     2401 caatcaacaa caatatgcat gttcacacac cccttt
//
LOCUS       DASZAK010000882         6650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_414108, whole genome shotgun sequence.
ACCESSION   DASZAK010000882 DASZAK010000000
VERSION     DASZAK010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414108"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VGT44_15480"
     CDS             <1..573
                     /locus_tag="VGT44_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582255.1"
                     /translation="QAQLQVKDQHSTPIPDVQSNATPLPAEVLTQAPPVGQALPSPTV
                     QGTLTLQVKVLVVRGAAILDAALQQLNQVARKMNPQYVVTTAKGPIQVSGIAASSSKG
                     GTLLTITYNAASQIVPYFNADDLSSLIAGKTEDQAKSSISSNEQGVLNVQSYDFNVFP
                     SFLNLLPFRPDHIHIIERPGIELPSGKPNG"
     gene            644..1150
                     /gene="ruvX"
                     /locus_tag="VGT44_15485"
     CDS             644..1150
                     /gene="ruvX"
                     /locus_tag="VGT44_15485"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV2582256.1"
                     /translation="MVRLVALDVGEVRIGVAVSDASGFLASPYTTLRVSRDQAQIWQA
                     IQRIVDETGAEGLVVGLPISLDGQIHTQGERVRDFAGRLREHIQVPLTFWDERFSTVE
                     AQRLLAERTQGAYSPGKPGGRERRQGQRGKRGRQGRQHEIDALAAAVILQEYLDSSKA
                     NENKDKNI"
     gene            1147..2256
                     /gene="mltG"
                     /locus_tag="VGT44_15490"
     CDS             1147..2256
                     /gene="mltG"
                     /locus_tag="VGT44_15490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV2582257.1"
                     /translation="MKRRGARASIFAVLLLGLLIFGGVYFAWTTVSDVFQPANANASN
                     KIAFVIQSGETGTQIADQLQAKGLIRNALAFRVWVKIKGVDTTQFKAGAYNLSPNMTI
                     DQIISQLLLGQPDEVPVVILEGWRLEQTANQFAHNNPVYQPLVKFRIADFLNIVRNPA
                     KFQDRAKYPFLQSIPAGNSMEGFLFPDTYLIPVDATANDVVDILLNNFASKVQQDHLD
                     TLAKQNKLTLYQMVTLASIVQREALFDADRPLVASVYWNRIYRPNAETVSELDADPTV
                     QYARDSQPGTTKYWTPLNDSGRNILPTNPYNTYTHQGFPPSPICSPGLASLLAAANPA
                     KSDYYFFLSKSNGQNVYAKTAAEFQADEQKYLGIS"
     gene            2271..3176
                     /locus_tag="VGT44_15495"
     CDS             2271..3176
                     /locus_tag="VGT44_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV2582258.1"
                     /translation="MNLTIWQTIALALLQGITELFPISSLGHTVILPGLLGWAGIETD
                     TSCGGQSCFLPLVTALHLGTSVALLAFFWRDWFQIILTLLKNVKEGEVRTGTEDWVSW
                     LIIIGVIPTGLIGLILQEPLKKLFSSPLIAASFLVVNGSILFAIERWRQYREAGMAFA
                     SAKEREASFRPLSSLNWKEAIVVGLAQSLALIPGISRSGMSMVAGLGVRLNHEDAARF
                     SFLLGTPIIFAAGALEIPQLIGQSSHTWTLVILGAALSGVAAFLSTKFLMKYFETGRL
                     NPFAYYCWGAGLLSLVLLILLHRTA"
     gene            3342..3542
                     /locus_tag="VGT44_15500"
     CDS             3342..3542
                     /locus_tag="VGT44_15500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582259.1"
                     /translation="MKNPIIFYAVIALGVIGLAAGVYYQFIDTAHHHTLRAPVGLAGG
                     AVLLIIGIVGLFMTRSKVAVAN"
     gene            3576..3794
                     /locus_tag="VGT44_15505"
     CDS             3576..3794
                     /locus_tag="VGT44_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582260.1"
                     /translation="MKNPALFFGAIVVAVIAFGLGVFYLVPNVYHPFTFGSHPPMDPQ
                     PTHAIAFFIVGVLLVLVALVNRPKTAVR"
     gene            complement(3872..5467)
                     /locus_tag="VGT44_15510"
     CDS             complement(3872..5467)
                     /locus_tag="VGT44_15510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582261.1"
                     /translation="MSTPTVSKWLTLIAACLGLTMLYIDLFIVNVALPSLEHELHTSL
                     STVSWTISAYALMIGVFPMGMGRLADLWGQRKLYFAGLVIFTLASLACGFAHDILSLI
                     VFRVIQGIGAAIMTPGTLAIVVRAFPLRQRGLAVGIYGGISGLGLIAGPALGGLLVQS
                     AGWRWVFFINVPIGLIALAMTMLFVPESRDEQASPHVDWVGLGLLCAGLLCVMFACTQ
                     AGTAGWTSFSVVGSGVIGIICLTIFVAVERRVRAPLVDLSLFRSRAFTLGNASFFLFS
                     AALFGLQPYMSLFMQNYWGFSPLQGGLAFLPATGLIAALTPVAGIIGQRAGSRLRIVG
                     AIGLLAMGLSSLYVAWTVGLQGSYTSSFLPALLARGVGIPLITTSATLAVMGAVSAGK
                     SGLAAGTLGMTRNIGTALGVAVFGQVFLGQVGDLLAGNLGFLSTNQAALVRIAAGQFL
                     VRGSGASQHIAGQAIVQGFATIMLVAAILCGLAFVVVCCMRTSASKDAEQHHDEVDRR
                     ELLQHGELNQTLASRALPGMTEL"
     gene            5708..6289
                     /locus_tag="VGT44_15515"
     CDS             5708..6289
                     /locus_tag="VGT44_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEV2582262.1"
                     /translation="MKEKQTKSAPEALRIVQDFVNTRYGRERRFDLTSPERLRVWLVQ
                     HGLLADGAPVTEGDLRRALDVREGLRGLMGVNNGMPMPDERLAGLNAIAKHSPLIVRF
                     SGDGQAQLEPDIAGVDGALARLMGIVYTAMHEGTWNRLKVCRNEPCMKAFYDTSKNHS
                     GTWCSMNKCGNRINARAYRHRHDQIAQSSSGEV"
     gene            complement(6275..>6650)
                     /locus_tag="VGT44_15520"
     CDS             complement(6275..>6650)
                     /locus_tag="VGT44_15520"
                     /inference="COORDINATES: protein motif:HMM:NF014908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582263.1"
                     /translation="SYTRMVQKEHGLIDWSRPAETLARQVRAYQPWPGAYTHWRGKLL
                     KILAAHALADQPGALVGQEMQAGTVILRKEGGQNALLVATGEGMLAIERLQLEGKKAV
                     STEEFLRGYPHIAGNILGSDLT"
BASE COUNT         1518 a         1952 c         1809 g         1371 t
ORIGIN      
        1 caagcccagc tacaggtgaa ggatcagcat agcacaccca tcccggatgt gcagagcaac
       61 gctacacccc tgcccgccga ggttctcacc caggcgccgc ccgttgggca ggctctcccc
      121 agtccaaccg tccagggtac gctaaccctc caggtcaaag tgctggtcgt gcgaggggcc
      181 gcgattctgg acgccgcgct tcagcagctt aaccaggtcg cacgcaaaat gaatcctcaa
      241 tacgtcgtca cgactgcgaa aggacctatc caggtcagcg gcatcgccgc cagcagttcc
      301 aaaggtggca cactgctgac catcacctac aatgcggcaa gccagatcgt accatatttc
      361 aacgctgacg atttgagcag cctgatcgcg ggtaagacgg aggaccaggc gaagagcagc
      421 atttcgagta acgagcaggg tgtcctgaat gtgcaaagct acgatttcaa cgttttccct
      481 tcattcctga accttctgcc cttccgcccg gatcacatcc atatcattga gcgacccggc
      541 attgagctgc catctgggaa accgaacggg taggaatcgt agacaaatgc ccctctcctt
      601 cgttaaaatg ctatagacga attgcaatac gagggagagc agtatggtac ggctggtagc
      661 actggatgtc ggagaggtga gaattggcgt cgcggtcagc gacgcctccg ggtttttagc
      721 ttcgccctat accaccctgc gcgtctcccg cgatcaagca cagatatggc aagccattca
      781 acgcatagtg gacgaaaccg gggcagaggg cctggtcgtc gggctgccga tcagcctgga
      841 cgggcagatt cacacccagg gggagcgcgt gcgcgacttt gccgggcgct tgcgagagca
      901 tatccaggtc cctctaacct tctgggatga gcgattctcg accgtagagg cacagcgtct
      961 gcttgccgaa cgcactcagg gtgcttactc accgggcaaa ccaggcggac gagaaagacg
     1021 gcagggacaa cgcggcaagc gcggcaggca gggaagacag catgagatcg atgccctggc
     1081 ggcggcggtg attctgcaag aatacctgga ttcgagcaag gcgaacgaga ataaggataa
     1141 aaatatatga aacgacgcgg ggcgcgcgca tcaattttcg cagtgttgct gctgggcctg
     1201 ctcattttcg gaggcgttta ctttgcctgg acaacggttt cagatgtgtt tcaaccagcg
     1261 aacgccaatg ccagtaataa aatagcgttt gtcattcaaa gcggggagac aggcacacaa
     1321 atcgccgatc aactccaggc aaaggggctg attcgtaacg cgctggcctt ccgcgtctgg
     1381 gtaaagatca agggtgtgga cactactcaa ttcaaggcag gagcctacaa cctcagcccc
     1441 aacatgacga ttgaccagat catctcgcag ttgttgctgg gccagcctga cgaggttccc
     1501 gtcgtcatcc tggaaggatg gcgattggag caaactgcca accagttcgc gcataataac
     1561 ccggtctacc agccgctcgt gaaattccgg atcgcggact tcttgaacat tgtgcgaaat
     1621 cccgccaaat tccaggatcg ggcgaagtat ccattcctgc aatctattcc ggcgggcaac
     1681 agcatggaag gcttcctgtt ccccgatacc tacctgatac cggttgacgc aaccgccaac
     1741 gatgtcgtcg atatcctgct gaacaacttt gccagcaaag tgcagcagga ccacctggat
     1801 accctggcga aacagaataa gctgacgctc tatcaaatgg tcacgctggc ctcaattgtg
     1861 cagcgcgaag cccttttcga cgctgatcgc ccattggtcg ccagcgtcta ctggaatcgc
     1921 atctaccgac ccaacgctga gacggtgagt gagttagatg ctgatccgac ggtgcaatat
     1981 gcgcgtgatt cgcagcccgg cacaacgaag tactggacgc cgctgaacga ttccgggcgt
     2041 aatatcctgc caaccaaccc gtataatact tacacccacc agggcttccc gccttcgccg
     2101 atttgcagcc ccggcctcgc aagcctgctg gcggccgcca acccggcgaa atcagattac
     2161 tacttctttc tctccaaatc gaatggacaa aatgtctacg cgaagacggc agcagaattt
     2221 caggccgatg aacagaaata cttaggtatc agttaagaga gaactaccgt atgaatttaa
     2281 ctatctggca aacgattgcc ctggccctac tccagggtat aaccgaactg ttcccgatca
     2341 gcagcctggg ccataccgtg atcctgcccg gcctgctcgg ttgggccggc atcgaaaccg
     2401 ataccagttg cggcgggcaa agctgtttcc tgccgctcgt gaccgcgctg cacctgggaa
     2461 ccagcgtcgc gctgctggca ttcttctggc gcgactggtt ccagatcatc ctgacgctcc
     2521 tcaaaaacgt taaagagggt gaggtgcgca caggcaccga agactgggtg agctggctca
     2581 tcatcattgg cgttatcccg accggtttga tcggccttat cctccaggaa ccgttgaaga
     2641 aactcttctc ctcgccgttg atcgctgcct cgttcctggt ggtaaacggc tcaatcctgt
     2701 tcgctattga acgctggcga cagtacagag aagccggcat ggcatttgcc agcgcgaaag
     2761 agcgcgaagc cagttttcgc ccgctctctt cactcaactg gaaagaagcc atcgtcgtcg
     2821 gcctggcgca gtcgctcgcg ctgatccccg gcatctcgcg ttccggtatg agcatggtag
     2881 ccggtctggg tgtgcgactc aaccacgagg atgccgcccg tttctccttc ctgctgggaa
     2941 cgcccatcat cttcgcggca ggcgcgctgg agatcccgca gctgatcggc caatcgtcgc
     3001 atacatggac gctggtcatc ctcggcgcag cactttccgg cgtcgcggcc ttcctgagta
     3061 caaaattcct catgaaatac tttgaaacgg gccggctcaa tcccttcgcc tattactgct
     3121 ggggtgctgg cctgctttcg ctcgttttgc tcattctctt gcacaggaca gcttaaacca
     3181 ctaatcacca aaaatggcta tctagacggc gagtgtgcag cttgcagcag ttgatacgtt
     3241 tggtacaata gatgcagttc ctgaaaaggt caggacgcac ggacaacaat ttattttgga
     3301 cgtattcact atcgtcccta agaaaaggta ggaacaactt gttgaagaac cccatcattt
     3361 tctatgctgt gattgccctg ggtgttatcg gtctggccgc cggtgtttac tatcagttta
     3421 tcgacaccgc tcatcaccat accctgcgcg ctccggtcgg acttgcgggc ggcgcagtcc
     3481 tgcttatcat cggcatcgtc ggcctgttca tgactcgctc aaaagttgct gtcgcgaact
     3541 agatcagcct tgtttagaaa ggtttcaaat cgattatgaa gaacccggct ctcttcttcg
     3601 gcgcgatcgt tgtcgcagtg attgcttttg gtctcggcgt cttttacctc gttccgaacg
     3661 tttatcatcc ctttacgttc ggtagccatc cgccgatgga cccgcaacct actcatgcca
     3721 tcgccttctt tattgttggt gtgctgctcg tactcgtcgc cctggtcaat cgccccaaaa
     3781 ccgctgtccg ctaacaaaac atccccgtag gggcggggga tgtcgcggat gtggggtgga
     3841 ggcgcttgcg tcgcccgcgt gcctggtgtt ttcacaattc ggtcatgcca ggcaacgctc
     3901 gtgacgcaag cgtctgattc aattcaccgt gttgcaacag ctcccgccgg tccacttcgt
     3961 cgtggtgctg ctcagcatct ttgcttgctg aggtacgcat acaacatacc acgacgaagg
     4021 ccaggccgca caagatcgcc gctacgagca tgatggtggc aaatccctgt actatcgcct
     4081 gtcccgcaat gtgctgactt gctcccgacc cccgtaccaa aaactgcccc gctgctattc
     4141 tcaccaacgc tgcctggttt gtcgaaagga agcccaggtt acctgcaagc aaatctccaa
     4201 cctggccaag aaagacctgg ccgaacacgg ccacgcccag cgccgtcccg atattgcgcg
     4261 tcatgccgag cgttcctgca gccagaccgg acttacccgc tgataccgca cccataacag
     4321 ccagcgtcgc gctggtagta atgagtggga tgccgactcc gcgcgcaagc aatgcaggca
     4381 ggaagctgct cgtgtaacta ccctgcaggc caaccgtcca ggcgacatac aaggaactca
     4441 gacccatcgc gagcagaccg atggccccaa caatgcgcag ccgcgaacct gctcgctgcc
     4501 cgatgatccc tgcgacgggc gtcagggcgg caatcagccc cgtggccggc aggaaagcca
     4561 gccctccttg cagtggcgag aagccccagt agttctgcat gaacaggctc atgtacggtt
     4621 gtaacccgaa gagagcggcg gagaataaga agaaactggc gttgccgagg gtaaaggcgc
     4681 gtgagcgaaa gagggaaaga tcgaccagcg gcgcgcggac acgccgctcg accgccacaa
     4741 agatggtgag acaaatgatc ccgatgaccc ccgatcctac cacggaaaag ctggtccatc
     4801 ctgccgttcc ggcttgcgta cacgcgaaca ttacacataa aagacctgca cagagtagcc
     4861 ccagtcccac ccaatcgacg tgtggagacg cctgctcatc tcgcgattct ggcacaaaga
     4921 gcattgtcat cgcgagtgcg atcagcccga tcggcacatt gatgaagaac acccagcgcc
     4981 atcccgcgct ttgcaccagg agaccgccaa gggcaggtcc tgcgatcagg ccaagtccgg
     5041 atatgccgcc gtaaatccca acggccaggc cgcgctgtcg caggggaaaa gcccgcacga
     5101 cgattgccag cgtacccggt gtcataatcg cagcgccgat cccctggatg acgcgaaaca
     5161 caatcaggga gaggatgtcg tgggcaaacc cacatgcaag cgatgccagc gtaaatatta
     5221 cgagaccagc gaaatacagc ttgcgttggc cccagagatc cgcgagacgc cccataccca
     5281 taggaaaaac accaatcatc agcgcatatg ccgaaatcgt ccaggaaacg gtgctgaggg
     5341 atgtatgcaa ttcatgctcc agcgaaggca aggccacgtt cacaatgaac agatcgatat
     5401 agagcatggt caagcccaga caggccgcga tcaaggtgag ccacttcgag accgtaggcg
     5461 tggacaaagg gggcgcctcc ccctcgggcg aatggaagtc gcgcgtcata gaatcctccg
     5521 aaattcctgc tgagttgccg ttgtcatgtg gagcatagat tttttctttc tcctcgtaca
     5581 cagtataacc aatgtaaagt cactagtcaa tatattttga tagtgatatt ttggcagaaa
     5641 acattgatat ttcattgacg aatagggtat actggtgata tgacctgaga aatgaggaac
     5701 acagtctatg aaagagaagc agacgaagtc agcgccagaa gcgctgcgca tcgtgcaaga
     5761 ctttgtgaat acacgctatg ggcgcgaacg gcgcttcgat ctcaccagcc ctgagcggct
     5821 ccgggtgtgg ctggtgcagc atggtctctt agcggatggg gcgccggtca cggaaggcga
     5881 cctgcggcgg gcgctcgacg ttcgtgaggg attgcgcggt ctgatgggcg tcaacaatgg
     5941 gatgccgatg ccggacgagc gtctcgcagg gcttaatgct attgcgaaac actccccgct
     6001 cattgtgcgt ttttcaggtg atggacaggc gcagttggag ccagatatcg caggcgtcga
     6061 tggcgcgctt gcccggctga tgggaattgt gtatacagcg atgcacgagg gaacctggaa
     6121 tcggctcaaa gtctgtcgca acgagccatg tatgaaggca ttctacgata cctccaagaa
     6181 ccactcagga acctggtgtt ctatgaataa gtgcggaaac cgcattaatg cgcgtgccta
     6241 tcgtcaccgt catgaccaaa ttgctcaatc atcctcaggt gaggtctgat cccaggatat
     6301 taccggcaat atggggatag ccgcgcagga attcttccgt actgaccgcc tttttgcctt
     6361 cgagctgcag gcgctctatc gccagcatcc cttcgcccgt tgcgaccagc agcgcgttct
     6421 gcccgccttc ctttcgcaag atgaccgttc ccgcttgcat ttcctgtcct actagcgctc
     6481 cgggctggtc tgccagggca tgagccgcga ggattttcag gagtttgccg cgccaatggg
     6541 tatacgctcc cggccagggc tgataggcgc gcacctgccg cgccagcgtc tccgcagggc
     6601 gactccaatc gatcaggcca tgctctttct gcaccatgcg cgtgtacgat
//
LOCUS       DASZAK010000883         3163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_554579, whole genome shotgun sequence.
ACCESSION   DASZAK010000883 DASZAK010000000
VERSION     DASZAK010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554579"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1339)
                     /locus_tag="VGT44_15525"
     CDS             complement(68..1339)
                     /locus_tag="VGT44_15525"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2582264.1"
                     /translation="MATRKFLPACFLVFAAVLMMLSGCASSPSSGPTTTGSTLKSGPG
                     VDTTTKTITLGILSPYSGPVAAPIGIPLADGVKAFFDHVNDNGGIDGYKVKFLEEDTQ
                     YNPQLEVQLYNQIHNQVLMIADSLGTPTTFAIKDLAAADHMLVSAATLSSALAREKYL
                     ILVGTPYRLQVENAFDYVVNTRGIKNPSTGIIYQNDEYGQDGLTGYKEAISAYGLHDV
                     AQVTYAATDTNFTAQVSKLKASGAKYVFVTAIPTAAAGILGTAFQLGYNPQWILQSPA
                     FARGLLAVPALVPLFSKAWIVSQGATWGDTSVPGMAQLLQDVAKYFPSQQPDGYFEFG
                     YTESYVTYDILKKAFDNNDVTRDGLFNAFQSLHAVSLGGLYPPVTYGSAPNDRVPTRD
                     SIVYQIDPTAPADVKALSPDFIGTAAAASQF"
     gene            complement(1450..2706)
                     /locus_tag="VGT44_15530"
     CDS             complement(1450..2706)
                     /locus_tag="VGT44_15530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2582265.1"
                     /translation="MSENNDVSNKPLERLAHARSERRTSQPPRLMATPMRWIVAGALT
                     FVIIVFFSVVSDDWITIVNYTMIAAIAALGLNVLSGYTGQVSLGIAFFMSIGAYTAAL
                     LGGNQPTSSFDPNGLALPWLIWLPAAGIIAALVGALIGPSALRLKGFYLGIVSLSLIF
                     IGQYIFKSAAGISGGPQGRTFPIPTFGDSAINQQNDLFGIALTAAQQYFLFLLPILVI
                     AALFVGNVMRSRAGRAMRAVRDNEIGASIMGVNLFQTKMGAFILSSFLAGIAGALYSS
                     YNSYVTPDYWSLTLSIQFVAAIIVGGVASVWGSILGAAFVFGLPLVIDQFKLLPLATS
                     SGGMTSGDLNAIIYGLLIIAFLLFEPGGIMSLAHRIQVWYNKLYRSQKKGGETGEITD
                     LNDLMDLSQDTHFAQEDAALPIVDNE"
     gene            complement(2703..>3163)
                     /locus_tag="VGT44_15535"
     CDS             complement(2703..>3163)
                     /locus_tag="VGT44_15535"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2582266.1"
                     /translation="VTIILCAALFIFFRYTKYGLSMRATALDQEAALAMGINIRRIYA
                     LAWGMSAAIATLGGVFLAIDSFAIDPTFGGTALLAFPAIILGGIDSVSGAVVGGIIIG
                     LAQVVTAGYESSVTSLPLNGIHEIMPYIIMILVLFVRPYGLFGTKKVERI"
BASE COUNT          679 a          829 c         1047 g          608 t
ORIGIN      
        1 cgacgcaagc gtccccaacc acaccgcgtc cgcccccgcc cctacggatg gtggcgaggg
       61 cggtttgtta gaactgcgat gcggcggcgg cggtgccgat gaagtcgggg gacagcgctt
      121 tcacgtctgc gggagctgtc ggatcgatct gatacacaat cgagtcgcgc gtggggacac
      181 gatcattggg cgctgaccca tacgtgacag ggggatacag cccaccgagg ctgacggcgt
      241 gcagcgactg gaaggcgttg aagaggccgt cgcgggtgac atcgttgttg tcaaacgcct
      301 ttttcaggat gtcgtaggtc acatacgact cggtgtagcc gaactcgaag tagccatccg
      361 gctgctgtga agggaagtac ttcgcgacat cctgaagcag ctgcgccatg ccggggacgc
      421 tggtatcacc ccaggttgcg ccctgcgaga cgatccatgc tttgctgaac agcggcacaa
      481 gggcggggac agccagcaag cctctggcga atgccgggct ttgcagaatc cactgcggat
      541 tgtagccaag ctggaaggcg gtgccgagga tgcctgctgc cgccgttggg atggcggtga
      601 cgaagacata tttcgcgccg gatgccttca acttcgaaac ctgcgcggtg aaattggtat
      661 cggtcgcggc ataggtgacc tgggcgacat cgtgcagacc gtaggcgctg atggcttctt
      721 tgtagcctgt cagcccatct tgaccgtact cgtcattctg gtaaatgatg ccggtcgagg
      781 ggtttttgat gccgcgcgta ttgaccacat agtcaaaggc attctcgacc tgcaggcgat
      841 agggcgtgcc gaccaggatg agatattttt cgcgagccag ggccgaggag agcgtcgcgg
      901 cggaaacgag catgtgatcg gcggcggcca ggtctttgat ggcgaaggtc gtgggcgttc
      961 ccaggctatc ggcgatcatg agcacctggt tgtgaatctg gttataaagc tgcacctcga
     1021 gttgtgggtt gtactgcgtg tcctcttcga ggaatttgac tttgtaaccg tcaataccgc
     1081 cattgtcgtt gacgtgatcg aagaaggctt tcacgccgtc cgccagggga atgccgatgg
     1141 gcgcggcgac ggggccggaa tagggcgaga ggatgcccag cgtgatagtt ttggtggtgg
     1201 tatcgacgcc ggggccggat ttaagcgtgc tgcccgttgt agtcggtccc gaactggggg
     1261 aactggcgca tcctgagagc atcatcaaga ctgcggcaaa gacgagaaag caagcgggca
     1321 ggaatttcct ggttgccata agtgactccc ctttgaattc aatcgagaat tgttgcgcga
     1381 agcaacacta cgcgacgttt gtcgtacttg accgaacaac ctttccgtat tcacgcgggc
     1441 gcggtttgac tattcgttgt caacgattgg aagtgcagcg tcttcctgcg cgaagtgggt
     1501 gtcctgtgac aggtccatca ggtcgttcag gtctgtgatc tcgccggttt cgcctccttt
     1561 cttctgcgat cgatacaact tgttgtacca gacctggatt cgatgagcca gactcataat
     1621 gcctcctggc tcaaagagca ggaaggcgat aataagcaag ccgtagatga tggcgttcag
     1681 gtcgccgctg gtcatgccgc cagagctggt ggcgagcggt aacagcttga actggtcgat
     1741 caccaggggc aagccaaaga cgaaggctgc ccccaggatc gatccccaga cgctggcaac
     1801 gccaccgaca atgatggcgg ctacaaactg aatggagagc gtcaggctcc agtaatctgg
     1861 agtgacgtag ctgttgtacg aagagtagag cgcgcccgcg atgcctgcca ggaaagacga
     1921 caggataaac gcgcccatct tggtctggaa caggttcacg cccataatgg acgcgccgat
     1981 ttcgttatcg cgcaccgcgc gcatggcgcg gcctgcgcgc gaacgcatga cgttgccgac
     2041 aaagagcgcg gcgatcacca ggatgggcag caggaacagg aaatattgtt gggccgcagt
     2101 cagggctatg ccgaacaggt cgttctgctg attgatggcg gaatcgccga aggttggtat
     2161 ggggaaggtg cggccctgtg ggccaccgga gatgcccgcg gcgctcttaa agatgtattg
     2221 cccgatgaag atcagcgaga gcgagacgat gccgagataa aagcccttga gacgcagcgc
     2281 cgatgggccg atcagcgcgc caaccagcgc ggcaatgatg cccgccgccg ggagccagat
     2341 caaccacggc agggccaggc cgttcgggtc gaacgagctg gtgggctggt tgccgcccag
     2401 cagcgcggcg gtgtaggcgc cgatggacat aaagaaggcg atgcccagtg acacctggcc
     2461 ggtatagccg gagaggacgt ttagaccgag cgccgcgatg gctgcgatca tggtgtagtt
     2521 gacgatggtg atccagtcat cgctgacgac ggaaaagaaa acgataatga caaatgttag
     2581 cgcgcccgcg acgatccagc gcataggggt tgccatgaga cgcggcggct gggaagtccg
     2641 ccgttcgctg cgcgcgtgag cgagacgctc gagaggctta tttgacacgt cgttgttttc
     2701 gctcatatgc gttccacctt ctttgtgcca aatagtccgt aggggcgcac aaacagaaca
     2761 aggatcataa tgatgtaggg catgatctcg tgaatgccgt tcaaaggcag gctcgtcacg
     2821 ctactttcat agccggcggt aactacctgg gccaggccga tgatgatgcc gcccacaacg
     2881 gctccgctta ccgagtcgat gccgcccagg atgatggcgg gaaaggccag cagcgcggtg
     2941 ccgccgaagg tggggtcgat ggcgaaagaa tcgatggcga ggaagacgcc gccgagagtg
     3001 gcgatggcgg cagacatacc ccaggccagg gcgtagatgc gacggatgtt gatgcccata
     3061 gccagggccg cttcctggtc cagcgcggtg gctcgcatcg aaaggccgta tttggtgtag
     3121 cggaagaaaa taaagagcgc ggcgcagagg atgatggtga caa
//
LOCUS       DASZAK010000884         6150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_414137, whole genome shotgun sequence.
ACCESSION   DASZAK010000884 DASZAK010000000
VERSION     DASZAK010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414137"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VGT44_15540"
     CDS             complement(<1..1259)
                     /locus_tag="VGT44_15540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582267.1"
                     /translation="MTAKKARPSLTSPGDEAFIQDIMQQRQDIAETLRQSASRAQANQ
                     ALADIFQADEATQLGLLKALAKQRDTGAADMLLAIHELAPQKDVRKEARRALIQLAGS
                     KVYPSWAPGSEKPTAIAANYPPRFWRGLVTLQRESGQAQLTLCWEYGFEYSEARSLNF
                     LLEFWRDGVKDFINETGTKRAIEKHLQEQIKLYLEGEDGPIQTVDCTLAEGKRLLLEA
                     MDANEWRKTTPHKDFRHFMPTINQLILNAPAIDIDRGNTFIDPNLEPDEAVGNYLGGW
                     SLGDYGLCYDLFSGDSPVREGLDRDEWVDRRRDWADEARPQHYEPRVIREREQSQSAI
                     WLPGSFTQARATTRREIDTCWSLEMGDTPLSGTLPEMPFGTAVLRETERRWFWTSFTL
                     VQEDDVWRIQRITDEGANIQGLPLADL"
     gene            1470..1988
                     /locus_tag="VGT44_15545"
     CDS             1470..1988
                     /locus_tag="VGT44_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582268.1"
                     /translation="MAELSDSDTEFILQVARAVVEEVAPEELVLFPLMAEAFVHSPES
                     LLISKGPGGVELGMGAGELIVTWTPTILLLLQQAVLQGARDAVTEGTKELVTGVFGRV
                     RGLWQAKGRKQAATLPARGQDLSREELQLLRTFLYEAALQHGVKDARAQQIADCAVVK
                     WVTRPVSEVRQR"
     gene            1985..4996
                     /locus_tag="VGT44_15550"
     CDS             1985..4996
                     /locus_tag="VGT44_15550"
                     /inference="COORDINATES: protein motif:HMM:NF016733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF642 domain-containing protein"
                     /protein_id="HEV2582269.1"
                     /translation="MSMFSQPSTLRPHLGAFGTETDRRLVLLLFAIIAASLYILHIEL
                     ATTFLQGMFAHCLQTSLANPGLDFNALTPDGLPQVCSVVPERWQSLGMLLGLLLLLGL
                     AVLVYWFAPRWKIWRVGLMPLEQYQQATIVRNGERVHIVTFLEKVQAAYLPGVRLRYV
                     SNRRATAQRQPSVFGGPGQAYLLVPFASITLFQQDTAAFQVSVLHELAHFYHRDVQKV
                     HFARALTLAFALLILLPFLAFTLWSLAFAPTSLAPLAKMEFVLSQFWGTLVMAGLVWL
                     LYRAALRSREMAADVQASYWAGTDAVLAGVLQKLDARRAWWQVALSDHPDQRALNVQE
                     PGRLFRLRYWEALATGITASLVLPTSYFTAMSLFSALNLTGLSLPGLVLVGVVGSLLL
                     CGLVVAVIGQMTWRAMLLVKLRGGGQLVGTGRVGLCLAAGLVLGFLFSLQFTPFLVYY
                     HINWQTLAVYFGPWLLGLALGLAGFCRWLAVLAGTWLEVIDGPRMARRVSSVNLAVAS
                     VVLATGLAVVALLCLTTLGYLFPQGIDYHALLKPVELQATDVLGFYWLMLQWLVFDFL
                     PLPGVALGLTVLWVLPLLSWLWYRQRTPINRYVWALADARQQAPSTPAHSPFKWGLLG
                     ETALVLGVLFCALFLLFHRVSLALASAEQLYPYALALTALPLVVAALWQGGLAALLYR
                     EARGIVNGLVGAFLAGLVIAAGCFLVYGLFGGTLGYPVAGDQFWPFCLQVLAWGSIGA
                     YALLLALDSWPGKGQRRWASATVEVSSLETSPLPAVKSEHTRSGGRTSHEPRDGSRRR
                     RWLWPVSLLLIIIALLLPRYASKPTPVAFTGIVKNGDFEVPVAPVGQFNEYYAGHAFG
                     GWTVAAGSIDLVNQPYWQAGHGNQSVDMDGDSAGTIYQDLPTRAGATYTLSFLLAANP
                     ECGDTVKVLQIWWSGNLLDTIQVSPHGHNNQHMGWHNSLIYAAHTAVNLKFPYVVRAS
                     ANVTRLEFISLTPGNCGAVIDLVSVVETSVG"
     gene            complement(5028..>6150)
                     /locus_tag="VGT44_15555"
     CDS             complement(5028..>6150)
                     /locus_tag="VGT44_15555"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEV2582270.1"
                     /translation="RGFFEPPQFTNDGTSLDTNAASLLLQGRNPYTDSNILSLVRLFP
                     IQPYWTTPLRQGQLADSLAYPSTWELRSILDTDLKSGSAPEFESKVSYPALSFLTLVP
                     FVWAHDYNVLPLYLFCFLALVAIGWKVSRREMRPLIVLVSLANVGTWNSVVGGNVDLL
                     YLLFIVVAWLVMDQRWWSAIFLGLAVATKQPAWLFIPFYAILSYRRYGMQETVRRLGI
                     VGIIFLAFNLPFMLWNPQAWLSGVLAPVADPMFPMGVGLVGLGTTPLFSYLPSSAYTV
                     MEVVALLCCLGWYWRICKALPEAAMLLAFLPLFLAWRSLPSYFYVSALPILILMIARI
                     LPRRSDPSSTSDASASNVQAGDEVGRKRGQPGGMSTRAA"
BASE COUNT         1097 a         1682 c         2069 g         1302 t
ORIGIN      
        1 tccaggtcgg cgagcggcaa accctggatg ttcgcgcctt cgtcggtgat gcgctggatg
       61 cgccacacat cgtcttcctg cacgagcgta aagctggtcc agaaccagcg ccgctccgtt
      121 tcgcgcagca cggcggtgcc aaatggcatt tcgggcagtg tgccgctcaa tggcgtatcg
      181 cccatttcaa gcgaccagca ggtgtctatc tcgcgtcgcg tcgtggctcg cgcctgcgta
      241 aacgagccgg gcagccagat agcgctctgg ctctgctcgc gctcgcgtat taccctgggt
      301 tcatagtgct gcggacgcgc ctcgtcagcc cagtcgcggc gccggtcaac ccactcgtcg
      361 cgatccaatc cttcgcggac agggctatcg ccggagaata gatcatagca caggccataa
      421 tcgccgagcg accatccgcc gagatagttt ccgaccgctt cgtcgggttc caggttggga
      481 tcgatgaagg tattaccccg gtctatgtca atggcgggcg cgttcaggat caattgattg
      541 atggtcggca tgaagtgacg aaagtcttta tgcggcgtgg ttttacgcca ctcgttggca
      601 tccatcgcct ccaacaggag acgcttacct tcggccaggg tgcaatcaac cgtttgaatc
      661 ggtccatctt ccccttccag gtaaagcttg atttgttctt gcaggtgttt ctcgattgcg
      721 cgcttcgtgc cggtttcatt gataaagtct ttgacaccat cccgccagaa ttccagcagg
      781 aaattcaggc tgcgcgcctc gctatactcg aagccatatt cccagcacag cgtcaattgc
      841 gcctggcccg actcgcgttg cagcgtgacg agtcccctcc agaaacgcgg tgggtagttg
      901 gcggcaatcg cggtgggctt ttcggagccg ggcgcccagc tcggatagac cttgctgccc
      961 gccagttgga tcagcgcgcg acgggcttcc ttgcgcacgt ccttctgcgg cgccagctcg
     1021 tgtatggcca gcagcatatc ggctgcaccg gtgtcccgtt gtttcgccaa tgccttgagc
     1081 aggccaagct gcgtggcttc gtcggcctgg aagatgtcgg ccagcgcctg gttcgcctgc
     1141 gcgcggctgg cgctttgccg cagcgtctca gcgatatcct ggcgttgttg catgatgtcc
     1201 tgtatgaatg cctcatctcc tggcgaggtg agggatggtc tggctttttt ggctgtcatt
     1261 gatggtgtgc ttcctcttgt tgttgcgtga tagcgcgctc gctgtttccc ttcgattgta
     1321 tcatgttgag caaggggaca aaactgtagg ttaggcaacc ttcgatcaag ctcatgttac
     1381 atctcccact ctgttcgttt gaatagatga acatgcattt gttgagctat atacctttga
     1441 tgaccgataa gcgaaaggat cgccgtttta tggctgagtt atctgattct gatactgaat
     1501 ttattttgca ggtggcgcgg gcggtcgtgg aggaggttgc cccagaagaa ctggtattat
     1561 tccctttgat ggcagaggca tttgtgcaca gtcctgagag cctgctcatc agcaaggggc
     1621 ctggtggagt ggaactggga atgggcgcgg gcgaactgat cgtcacctgg acgccgacga
     1681 ttttgctgct gctgcaacag gccgtgctgc agggtgcgcg agacgcggtg acggagggga
     1741 cgaaggagct ggtgacgggg gtgttcgggc gtgtgcgcgg gctatggcag gcaaagggca
     1801 gaaagcaggc ggcgaccttg ccagcgcgtg ggcaggattt gagccgggag gaattgcaat
     1861 tgctgcgcac ctttttgtac gaggcagcgc tgcaacacgg tgtgaaggac gccagggcac
     1921 agcagatcgc ggactgcgcc gtagtgaaat gggtgaccag gccggtgagc gaggtacgcc
     1981 agcgatgagc atgttttccc aaccatctac gctgcgaccg cacctgggag cttttggcac
     2041 ggaaaccgac cgcagacttg tgctgctgct gtttgcgatt atagcagcca gcctgtatat
     2101 actgcatatt gaactggcga cgacattttt gcaggggatg tttgcgcatt gtcttcagac
     2161 ctcgctggcc aacccagggc tggatttcaa cgcgctgacg ccggatgggt tgccgcaggt
     2221 gtgtagtgtg gtgccggagc gctggcagag cctggggatg ctgctcgggc tgctgttgct
     2281 gctgggtctg gcagtgctgg tgtactggtt tgctccgcgc tggaagatct ggcgcgtcgg
     2341 gctgatgccg ctggagcagt atcagcaagc gacgattgtg cggaatggcg agcgggtgca
     2401 tatcgtgacg tttctggaga aggtgcaagc ggcctatctg ccgggggtgc gcctgcgcta
     2461 tgtcagcaac cggcgggcaa cggcgcagcg ccagccgtcg gtgttcggcg gacccggaca
     2521 ggcatatctc ctggtgccgt tcgcgtccat tacgctgttc cagcaagata cggcggcgtt
     2581 tcaggtaagt gtgctgcacg agctagcgca tttttaccac cgggatgtgc agaaagtaca
     2641 ttttgcacgg gcgttgaccc tggcgtttgc gctgctgatc ctgctgccat ttctggcctt
     2701 cacactctgg tcgctggcgt ttgcgccgac gagcctggcg ccattggcga agatggagtt
     2761 tgtactgagc cagttttggg ggacgctggt gatggcgggc ctggtctggc tgctttaccg
     2821 ggccgcactg cggtcgcggg aaatggcggc tgacgtgcag gccagctact gggccggtac
     2881 agacgcggtg ctggcgggcg tgctgcaaaa gctggacgcg cggcgggcgt ggtggcaggt
     2941 ggcgctgagc gaccatccag accagcgggc gctgaacgtg caggagccgg ggcggctgtt
     3001 tcgtctgcga tactgggagg cgctggcgac gggcatcaca gccagcctgg tcctgcccac
     3061 ctcctatttt accgcgatgt cgctattttc ggcgttgaac ctgaccggcc tttcgctgcc
     3121 ggggttggtc ctcgtcgggg tcgtcggttc gctgctgctg tgcgggctgg ttgtggccgt
     3181 gatagggcag atgacctggc gggcgatgct gctggtgaag ctgcgcggcg gcgggcaact
     3241 ggttgggact ggtcgcgtgg ggctttgcct ggcagcaggc ctggtgctgg ggtttctctt
     3301 ttcgctgcaa ttcacaccct tcctggtcta ttatcatatc aattggcaga cgctggcggt
     3361 ttattttggg ccgtggctgc taggactggc gctggggctg gcgggctttt gtcgctggct
     3421 ggctgtgctg gctggaacct ggctggaggt aattgacggg cccaggatgg cgcgccgggt
     3481 gagcagcgtc aacctggcgg tggcaagcgt ggtgctggcg accgggctgg cggtggtggc
     3541 cctgctctgc ctgacgacgc tgggctacct gtttccgcag ggcatcgatt atcacgcctt
     3601 gctcaagccg gtggagctac aggcgaccga tgtgctgggc ttttactggc tgatgctcca
     3661 gtggctggtc ttcgactttc tgccgctgcc cggcgtggcg ctgggactga ccgtgctgtg
     3721 ggtgctgccg ctattgagtt ggctctggta tcggcagcga acacccatca accgctacgt
     3781 ctgggcgctg gctgatgcac ggcagcaagc gccgtcaaca cctgcgcata gccccttcaa
     3841 gtggggactg ctgggcgaga cggcgctggt gctgggggta ttgttctgcg cgctgttttt
     3901 gctctttcac cgggtctcgc tggccctggc gagcgcggag caactctatc cctatgcgct
     3961 ggccctcacg gcgctgccgc tggtggtagc ggcgctgtgg cagggcgggc tggcagcgct
     4021 gctctaccgg gaagcgcgag ggatcgtaaa cgggctggtc ggcgcgtttc tggccgggct
     4081 ggtaatcgcg gcgggctgtt ttctggtcta cggcctgttt gggggaacgc tggggtaccc
     4141 ggtggcgggc gatcagttct ggccgttttg cttgcaagtg ctggcctggg gcagtatcgg
     4201 cgcctacgcg ctgctgttgg cgcttgacag ctggccgggg aaagggcagc gcaggtgggc
     4261 aagcgctaca gtagaagtga gcagcctgga gacatctccc ctgccggcgg tgaagagcga
     4321 acacacccgg tctggcgggc gaaccagcca cgagccacgc gacgggagca ggcggcggcg
     4381 ctggttatgg ccggtctcgc tgctgctgat catcattgcg ctgctgctgc cgcgttacgc
     4441 gagtaagcca acgccggtgg cctttacggg cattgtcaag aacggcgatt ttgaggtacc
     4501 cgtagcgcct gtagggcaat ttaacgaata ttacgccgga catgcctttg gtggctggac
     4561 cgtggcagca ggcagtattg acctggtaaa ccaaccttac tggcaggccg ggcatggcaa
     4621 ccagtcggtg gatatggatg gagatagcgc gggtacgatc tatcaggacc tgccgacgag
     4681 agcgggggcg acgtacaccc tttctttttt gttggcagcc aatcccgaat gtggagacac
     4741 agtcaaggtg ttgcagatct ggtggagcgg gaatctgctg gacacgatcc aggtaagccc
     4801 acatgggcat aacaatcagc acatgggctg gcataattcg ctgatctacg ctgcgcacac
     4861 agcggtgaat ctgaaattcc cctatgtggt gcgagccagc gccaatgtaa cacgcctgga
     4921 atttatcagc ctgacgccag gaaattgtgg cgcggtgatc gacctggtgt cggtggtgga
     4981 gacgagcgtg gggtagagag ccgggggcga tggcttacta tgctacctca cgccgctcgc
     5041 gtactcatcc caccgggctg cccacgcttc cggccaacct cgtcgccagc ctggacgttc
     5101 gaggcggacg catcgcttgt gcttgaggga tcgctccgcc tgggcagaat gcgcgctatc
     5161 atcagaatca aaatcggcag tgccgagacg tagaagtacg atggcaggct gcgccacgcc
     5221 aggaagagcg gcaggaaggc cagcagcatc gccgcctcgg gcagtgcctt gcagatgcgc
     5281 cagtaccagc cgaggcagca cagcagggca accacttcca tgaccgtata ggcgctggag
     5341 ggcagatagg agaataaagg cgtggttccc aatccgacca ggccgacgcc cattgggaac
     5401 atcggatcgg ccaccggggc aagcacgccc gaaagccagg cttgcgggtt ccagagcatg
     5461 aagggcaggt tgaatgccag aaagataatg ccaacaatcc ccaggcggcg cacggtctcc
     5521 tgcatcccat agcggcgata ggacaggatg gcatagaagg gaatgaataa ccaggccggt
     5581 tgtttggtcg ctaccgccag tcccaggaag atggccgacc accagcgttg atccatcaca
     5641 agccaggcga cgacgatgaa gagcaggtag agcagatcaa cgttgccgcc gacaacggaa
     5701 ttccacgtgc cgacattggc cagcgagacg agcacgatga gcgggcgcat ctcccgtcgg
     5761 gaaactttcc agccgatcgc caccagcgcc aggaaacaga acaggtagag gggcagcacg
     5821 ttgtagtcat gcgcccagac aaagggaaca agcgtcagaa acgagagcgc gggatagctg
     5881 actttcgact caaactctgg cgcgctgccc gatttgaggt cggtgtccag gatgctgcgc
     5941 agttcccagg tggaggggta cgccaggctg tccgccagtt gcccctggcg cagcggcgtc
     6001 gtccagtacg gctggatagg gaacaatcgc acgagcgaga ggatattgga gtcggtatag
     6061 gggttgcgcc cctgcaagag caacgaagca gcgttggtat ccagcgaggt gccatcgttg
     6121 gtgaactgcg gaggctcaaa aaatcccctg
//
LOCUS       DASZAK010000885         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_94670, whole genome shotgun sequence.
ACCESSION   DASZAK010000885 DASZAK010000000
VERSION     DASZAK010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94670"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VGT44_15560"
     CDS             complement(<1..258)
                     /locus_tag="VGT44_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582271.1"
                     /translation="MEKETQRIARQQLMEIEQQCLGLLTRQVARRVTQWYDQCFQPNG
                     LRSTQFNLLVAIALAQTVPLTRLAEILVLDRTTLARNLKPLE"
     gene            complement(290..1177)
                     /locus_tag="VGT44_15565"
     CDS             complement(290..1177)
                     /locus_tag="VGT44_15565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2582272.1"
                     /translation="MREFKPLQFGVNIHTRIQPGVNPVAEALHAEQLGFDVVTLHGDA
                     QHGSDPSYELWTLLSWVAAHTTSIRVAPVVLALPHRHPAVLAKMAETLDRLSGERLLL
                     VLGGGGPMNEPAYRALGLVQRSPREKVEALEEAVDLLRGLWRASGFSYPGQHFRTEEA
                     TIEPKPSHPIPVWLGVFGDRMLDLVGRKADGWLPTYQFLQPEQAFQKVEHIRAIAEYA
                     GRNPDEITYGYNIPVLVEEGAVTNRWRIAGSASKVAGQLATLARHGFTFFNMWPVGEG
                     FMQRERLIRDVLPIVRDLP"
     gene            complement(1207..2631)
                     /locus_tag="VGT44_15570"
     CDS             complement(1207..2631)
                     /locus_tag="VGT44_15570"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEV2582273.1"
                     /translation="MTHPTTSQLPAEARLLQRKSRVPRPPGPPWSTSTQNTFSFLRHA
                     LDFLLEMRERYGDVVSVPTLVGPFTLIFHPDGVRHVLQENHRNYNKDVPDYHVLSLVL
                     GKGLLTNDGGSWLRFRRLIQPAFHRERVVAFGTLMTETTLRWTQRWETGNFIETNQPL
                     ALTREMSSLTLSIVGNALFGTDLSAQNESVWRALTTVNHLLAKAFYLPWVLSLPTPQR
                     RRLHTARSALYTVVEQIIRERRRRRETHGDLLEMLLEARDDETGEGMTDQQVRDEVLT
                     LLLAGHETTANALSWTFYLLAQHPHIEARLQEEYQQVLNGRPAMIDDLPRLSFTRMVV
                     EEAMRLYPPAWGLGRRAIGNDEIGGYAIPKGAYVLVFPYVTHRHPAFWERPDVFDPER
                     FSAPLAAGRHRFAYFPFGGGPRLCLGNQFALYEAQLILATILSRYQLRPLPGAVVTPE
                     PLVTLRPRGDLLMTVHRTPEMKRR"
BASE COUNT          552 a          779 c          815 g          549 t
ORIGIN      
        1 ttccagcggc ttcagattgc gagccaacgt cgtgcgatcc agtaccagaa tctcagccag
       61 gcgtgtcagc ggcaccgttt gagcgagagc gatggccacc agcaaattga actgtgttga
      121 gcgcagcccg ttcggctgaa agcactggtc ataccattga gttaccctgc gagccacctg
      181 gcgtgtcaat agacccaggc attgctgctc aatctccatc agctgctgcc gcgcgatgcg
      241 ttgcgtttct ttctccatac taggagtata tacacgtaaa tggcttttgt caagggaggt
      301 cgcggacgat gggcagcacg tccctgatca atcgctcgcg ctgcataaat ccctcaccaa
      361 ccggccacat attgaaaaaa gtgaagccat gccgggcaag agtcgccaat tgcccggcca
      421 ccttactggc gctcccggca atgcgccatc ggtttgtgac agccccttcc tcgacgagga
      481 ccgggatatt gtacccgtat gtgatctcgt ccgggttgcg cccggcgtat tccgcaatgg
      541 ctcggatgtg ttctactttc tggaacgcct gctctggctg cagaaactgg tacgtcggca
      601 accagccgtc tgctttgcgc ccgacgaggt cgagcatccg atcaccgaac acaccgagcc
      661 agaccgggat cgggtggctg ggcttgggtt cgatcgtggc ttcctcggtt ctaaagtgct
      721 gtcctggata ggagaagccg gaggctctcc agagacctcg gaggaggtca acagcctctt
      781 cgagcgcctc caccttttcc cggggcgagc gctggacgag accaagggct cggtatgctg
      841 gctcattcat cggcccacca ccgcccagca cgaggaggag ccgctcgccc gagaggcgat
      901 cgagcgtctc ggccatcttg gccagcacgg cgggatgccg gtgcggcagg gccagcacaa
      961 ccggggccac ccggatgctg gtggtgtgag ccgccaccca ggagagcagc gtccacagct
     1021 cgtacgaagg gtcacttccg tgctgggcat cgccatggag cgtcaccacg tcgaagccga
     1081 gttgctcagc atgtagggcc tctgccaccg ggttcacgcc aggctgtatt cttgtgtgga
     1141 tgttcacccc aaactgcagg ggtttgaact ctctcaccat cttctttctc cttcctgctg
     1201 atcggatcag cgtcgtttca tctccggtgt tcggtgcacg gtcatcagca ggtcgccacg
     1261 ggggcgcagt gtcaccagcg gctcaggggt gacgactgcc ccaggcaggg gccgcagttg
     1321 atagcgtgac aggatcgtcg ccaggatcaa ctgcgcttca tagagagcaa actgattgcc
     1381 aagacacagg cgtgggccac ctccaaatgg gaagtaggca aagcggtggc gtcctgctgc
     1441 cagcggtgcc gagaaccgtt cgggatcgaa gacgtctggt cgctcccaga aggccggatg
     1501 acgatgcgtg acgtagggga agaccagcac gtaagctccc ttgggaatcg cgtagccacc
     1561 gatctcatca ttgccaatag cacgacggcc tagaccccag gctggtggat agaggcgcat
     1621 cgcttcctca accaccattc gagtaaagga gagccgtggc agatcatcaa tcatcgcagg
     1681 gcgtccgttc aacacctgct gatattcctc ctggagcctt gcttcgatgt gcggatgctg
     1741 ggcaaggagg tagaacgtcc acgagagcgc gttggcggtc gtttcgtgac cggccagcag
     1801 taacgttagc acttcgtcgc gcacctgttg atcagtcatg ccttcgcccg tttcatcatc
     1861 gcgtgcctcc aggagcatct cgaggagatc gccatgcgtt tcgcgacgcc tacgacgctc
     1921 gcgaatgatc tgctcaacga ccgtgtacag agcgctccgt gcagtgtgca agcggcgacg
     1981 ttgcggcgtt ggaagcgaca ggacccacgg caggtaaaac gccttcgcta acaggtgatt
     2041 aacagtagtg agtgctcgcc aaacgctctc gttctgtgcg ctcaggtcgg ttccaaagag
     2101 agcattaccg acgatgctta aggtcagact actcatctca cgcgtgagag caagcggctg
     2161 gttggtttcg atgaagttgc ctgtttccca gcgctgggtc cacctcaagg tagtctcggt
     2221 catgagcgtt ccaaaggcta cgactcgctc gcgatgaaag gcgggttgga tcaagcggcg
     2281 aaacctgagc cacgacccgc catcgttggt caggagacct ttcccaagga cgagactgag
     2341 aacatgatag tcagggacat ccttgttgta gttgcggtga ttctcctgga ggacgtggcg
     2401 caccccgtca ggatgaaaga tcagtgtgaa cggaccgacg agcgtaggaa cagagacgac
     2461 atctccatag cgttcgcgca tctccagaag gaagtcgagc gcatggcgca aaaaactgaa
     2521 cgtattctgg gtcgaggtag accatggtgg tcccggcggg cggggcacac gcgacttacg
     2581 ttgcagaaga cgcgcttcgg caggaagctg tgaagttgtg gggtgggtca tatcgtgttc
     2641 tcctctcagt gagtgtcgtt ttggccttgc tccgtctcaa cgcaaacgga tgact
//
LOCUS       DASZAK010000886         3028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_94761, whole genome shotgun sequence.
ACCESSION   DASZAK010000886 DASZAK010000000
VERSION     DASZAK010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94761"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2619
                     /locus_tag="VGT44_15575"
     CDS             <1..2619
                     /locus_tag="VGT44_15575"
                     /inference="COORDINATES: protein motif:HMM:NF042871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEV2582274.1"
                     /translation="SDLMSACDNDAALFERVLPFAGLLRTDTFGKPVVIAAGSVYVTQ
                     GSDRRSTGTHYTPRSLTEPIVQHALDPLVYLGPAEGKPQEEWQLRPAGDILKLKVCDF
                     AMGSGAFLVQVCRYLAEKLVEAWETALPPASGQPGQPTMPPRITPEGLPSSGDPGETL
                     LPADETERLALAMRLVAERCIYGVDKNSLAVEMAKLSLWLITLSKNKPFTFLDHALRR
                     GDSLLGVNLRQLTSWSMDASPASGAARQMVWIEPALKRALEVTLKWRRQISTMPENTV
                     RDIENKAGLLFQVEGAMAILKLGADLLIATTLEQDPKRRSMLQDTLHMDYTLLLSSYE
                     NSRTIRYTTAIWTMDDDAYNKLRDEVDNLLNGRRPFHWPLEFPEVFAGLEEERGFSAI
                     ISNPPFQGGQKITSALGTNYRDYLVEYLANGKRGSADLCAYFFLQASRLVREGGMCGL
                     LATNTIAQGDTREVGLDQIIAAGWSIPYAVPSRKWPGEASLEVAHIWLRHGNWHGAFI
                     LNDLPVEGISTFLTPQESVQGKPYRLFANADKSFIGSYVLGMGFVLEPQEALALIEKD
                     PRNRIVLYPYLNGEDLNSRPDQSPSRWVINFHDWPLEQAEMHPDCMKIVREKIKPERD
                     LNNRKVRREQWWLFAERASGLYSTIAGKERVLVIALTSRTCAFSFNPSDIVFSHATGV
                     FALDKFCHFAILQSAFHVEWAFYYASSMKGDLRYTPSDCFETYPFPLSLQGLDDIGER
                     CFQHRQAIMLERQEGLTKTYNRFHNPKVQAEEIIKLRVLHREMDEAVVRAYGWDDLTL
                     GHGFHETKLGVRYTISEEARSEVLGRLLRLNHERYAKEVVLGLHEKGAKKGKGKNSKG
                     KRGTEIEGVQGELVFE"
     gene            2646..>3028
                     /locus_tag="VGT44_15580"
     CDS             2646..>3028
                     /locus_tag="VGT44_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582275.1"
                     /translation="MKAELTINIRPVRHVYFIGENDLERFVEVASYCCTQWGGSNNLI
                     IPVQINKETDDAQVFVHSYNLFLIRSRYPDVFVDAFSDKEENSSIRDDLAKYFASEFP
                     GKNLQSWETFLLIDDVFHPLNILSP"
BASE COUNT          703 a          807 c          857 g          661 t
ORIGIN      
        1 tccgacctga tgagcgcctg cgataacgat gccgccctgt tcgagcgcgt gctgcccttc
       61 gccgggctgc tgcgcaccga caccttcggc aagcccgtcg ttatcgcggc aggatcggtc
      121 tacgtcacgc agggcagcga ccgccgctcc accggcacgc actacacgcc gcgcagcctg
      181 accgaaccga tcgtgcagca cgccctcgac ccgctcgtct accttggccc ggcggagggc
      241 aagccgcagg aggagtggca attgcgcccg gcaggcgaca tcctcaagct caaggtctgc
      301 gattttgcca tggggtcggg cgcgttcctg gtgcaggtct gccgctacct ggcggaaaag
      361 ctggtcgaag cctgggagac cgcgctaccg cctgcctccg gccagccagg gcagccaacc
      421 atgccgccgc gcatcacgcc ggagggtctt ccatcgagtg gcgaccccgg cgaaacgctg
      481 ctgcccgccg acgaaaccga gcggctggcc ctggcgatgc gcctcgtggc cgaacgctgc
      541 atctacggcg tagacaagaa ctcgctcgcc gtcgagatgg ccaagctctc gctctggctc
      601 atcacgctca gcaagaacaa acccttcacc ttcctcgacc atgccctgcg ccgcggcgac
      661 tcgctgcttg gcgtgaacct gcgccagctc acctcgtggt ctatggacgc ttcgcccgcg
      721 agcggcgcgg cgcggcagat ggtctggatc gaacctgcct tgaaacgcgc tttagaggtc
      781 acattgaagt ggcgtcgtca gatcagcaca atgccagaaa acactgtgcg tgatatagag
      841 aacaaagctg gattgctttt tcaggttgaa ggagctatgg ctatcctcaa actgggtgcg
      901 gacttactta ttgcgacaac tctggagcaa gaccccaaac gacgcagtat gttgcaggat
      961 acgttgcata tggattacac cttactgttg agtagctatg aaaattctcg cacaatacgc
     1021 tacactacag caatctggac aatggatgat gacgcttata acaaactacg cgacgaagtg
     1081 gataacctgc tcaacggtcg ccgcccgttc cactggccgc tggaattccc cgaagtattc
     1141 gccggtctgg aagaagaacg cggcttttcg gctatcatca gcaatccgcc attccagggt
     1201 ggtcagaaga tcaccagtgc gctcggtacc aattaccgcg attatcttgt cgaatacttg
     1261 gcgaacggca agcgtgggag cgccgatctc tgcgcgtact tcttcctgca agcgagtcga
     1321 ttagtgcgag aagggggcat gtgcgggctg ctggcaacga atacgatagc ccagggcgac
     1381 actcgcgagg ttggtctgga ccagatcatc gcagcaggtt ggagcatccc gtatgctgtg
     1441 ccaagccgca aatggccggg cgaagccagc ctggaggtgg cccatatctg gctgcggcac
     1501 ggcaactggc atggggcgtt catactgaac gacctgcctg tcgagggcat ctccaccttc
     1561 ctaacacctc aagaatcagt gcagggcaag ccctatcgcc tcttcgctaa cgctgacaaa
     1621 tcctttatag gctcttatgt gttaggcatg ggttttgtac tggaaccgca agaagctctg
     1681 gcgctgatcg aaaaagatcc gcgcaatcgc attgttctct acccctattt gaacggcgaa
     1741 gacctgaact cccgcccaga tcagtcgccg agccgctggg tcatcaactt ccacgattgg
     1801 ccactagaac aggctgagat gcatcctgat tgtatgaaga tcgtgaggga gaaaatcaag
     1861 ccagaacgag atctgaataa tcgtaaagtg aggcgtgagc aatggtggct ctttgctgaa
     1921 agagcatctg gcctctactc cacgattgcg gggaaggagc gggtgctggt tatagcacta
     1981 actagccgaa cgtgtgcttt ttcattcaat ccaagcgata ttgttttctc acatgctaca
     2041 ggtgtttttg ctcttgataa attttgccat tttgcaatac ttcaatcggc tttccacgta
     2101 gaatgggctt tttattatgc ttcatccatg aaaggtgact tgcgctatac cccatctgac
     2161 tgcttcgaaa cttatccatt tcctctgagc ttgcaaggac tcgacgatat tggcgaacgc
     2221 tgcttccaac acaggcaggc gattatgctg gagcggcagg agggattgac gaaaacgtac
     2281 aatcgtttcc acaacccgaa ggtgcaggcg gaagagatta tcaagctgcg tgtgctgcac
     2341 cgcgagatgg acgaggcggt ggtgcgtgcc tatggctggg atgacctgac gctagggcat
     2401 ggattccacg agacgaagct gggagtgaga tatacgatca gcgaggaagc gcggagcgag
     2461 gtactgggac ggctgctgcg gctaaaccac gagcggtacg cgaaggaggt ggtgctgggg
     2521 ctgcatgaga agggggcgaa gaaggggaaa gggaagaata gcaaagggaa gcgtgggacg
     2581 gagatagagg gcgttcaggg agagctggtg ttcgagtgaa tgtgttttgg aaagggtagc
     2641 attcaatgaa ggctgagcta actatcaata tacgtccagt acggcatgtc tatttcattg
     2701 gtgaaaacga tcttgagcgt tttgttgaag tcgcaagtta ttgctgcact cagtggggtg
     2761 gcagcaataa cctgataatt cctgttcaga tcaataagga aacagatgat gcccaagtct
     2821 ttgtacattc atacaacttg tttttaatca ggtcgcgata tcctgacgtc tttgtggatg
     2881 ctttttcaga caaggaagaa aatagctcaa tccgcgacga tctcgctaag tatttcgctt
     2941 ccgagttccc tggtaaaaac cttcaatcat gggaaacatt cttactaata gatgatgttt
     3001 ttcacccact gaatatactc tcccctca
//
LOCUS       DASZAK010000887         1560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_94765, whole genome shotgun sequence.
ACCESSION   DASZAK010000887 DASZAK010000000
VERSION     DASZAK010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94765"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..1477
                     /locus_tag="VGT44_15585"
     CDS             227..1477
                     /locus_tag="VGT44_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV2582276.1"
                     /translation="MSNPSVSSSLPNRWVLAGAAFCMQLALGAVYAWSVFLTPVMTSY
                     HATKPAANLTFTITLVALGITAGFGGYFNSRFGPRTIATVAAIFYGVGIFLAGFVSPN
                     LFLLYLTYGVIGGIGLGLGYIVALAMLIKWFPDRRGFITGLAVAGFGAGAAITGPVAS
                     YILLPAFKLNTTFTILGIAYFVIVILAAQFFRRAPDGYAPAGWTPSTQQQAVRSARDY
                     TLGEALSSPRWYVLWLILALNVTAGAALISVAAPLAHDFTGIALTAASTLVITISIFN
                     GAGRLFWGWVSDTIGRPYTFLSLFVIQIIAFIFAALIGQGQFALLFIPASIIGLCYGG
                     GFGTMPAFAADFFGPRNAGTIYGAMLTAWSAGAIVGPILIASVDFRTALYIIAVIMAV
                     STVLPIVARAMGRRQLQMEASAGQ"
BASE COUNT          262 a          472 c          411 g          415 t
ORIGIN      
        1 tttacatcat ctatacctta ctattacctt aacgcagtgg attattcaga ttgccgcttg
       61 tctctgcgaa aaaggctatc tgatgttatt gttttgtagt gctaacttga tgtagtgcgt
      121 tctcctagcg cgtctctgta gtgcattcac gactgcatca ttttatcgct taccctctct
      181 ccctgatcgt catttcattg tcgcaactga ccgagaggag tatctcatga gcaatccttc
      241 tgtttcgtcc tcccttccca atcgctgggt cctcgcaggc gccgccttct gtatgcagct
      301 agccctcggt gccgtgtatg cgtggagcgt attcctgaca cctgtgatga cctcgtacca
      361 cgcgaccaag ccagcggcga acctcacctt caccatcacg ctggtcgctc tcggcatcac
      421 cgccggtttt ggcgggtact tcaacagccg ttttggtcca cgcacgattg ccaccgtagc
      481 tgccatcttt tatggggtag gcatcttcct cgcgggattt gtctcaccca acctgtttct
      541 gctctacctg acctatggtg tgattggtgg catcggcctg ggactcggct atatcgtcgc
      601 gctggccatg ctcatcaagt ggtttcctga ccgccggggt ttcattaccg gcctggccgt
      661 cgctggattt ggagctggag cggctattac cggcccggtt gccagttaca tcctgcttcc
      721 tgcttttaag ctgaatacga ctttcacgat cctcggcatc gcctacttcg tgatcgttat
      781 tctcgcggcc cagttcttcc gcagagcgcc ggatggctat gcccctgcgg gctggactcc
      841 ctcaacgcaa cagcaggctg tccgttcagc gcgcgactat acattgggtg aggcgttgag
      901 cagcccacgg tggtatgtgc tgtggctcat tctcgcgctg aatgtcactg ctggcgctgc
      961 gctcatctcg gtggccgctc ccctggcgca cgactttact ggcatcgctc tgacagcggc
     1021 ttccacgctc gtgattacga tctccatctt caacggcgcg ggccgcctgt tctggggctg
     1081 ggtatcggac acaataggcc gcccgtacac cttcctgagc ctgttcgtca tccagattat
     1141 cgccttcatc ttcgcagcgc tgatcggcca gggccagttc gccttgctct ttataccagc
     1201 ctcgatcatc ggcctctgct acggaggtgg ctttggcacg atgccagcct tcgccgctga
     1261 cttcttcggg ccgcgcaacg caggcaccat ttatggcgca atgctgacgg catggagcgc
     1321 gggcgcgatt gtcggtccaa tcctgatcgc aagcgtcgat tttaggacgg cactctacat
     1381 cattgctgtg attatggccg taagtaccgt cttgcccatc gttgcccgcg caatgggtcg
     1441 gcggcaatta caaatggagg cttctgcggg gcaataaccc ccctcgtagg acgcgatgat
     1501 gaagtttcac gagcaagatc ggtaggaagt tgtcatcctg agcgcagcga aggatctcgg
//
LOCUS       DASZAK010000888         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_306392, whole genome shotgun sequence.
ACCESSION   DASZAK010000888 DASZAK010000000
VERSION     DASZAK010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306392"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VGT44_15590"
     CDS             complement(<1..498)
                     /locus_tag="VGT44_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEV2582277.1"
                     /translation="MTTPFVIDNQQHSMAAVLNDLLAQHRSHSLDIATAYFNVGGWQL
                     LREGLDGLGAFRLLLGDEPEAGVDLGLRDIGAKPVKGLIHELAKEPFNAQTLRQVEDL
                     IAFLRRPQVQVRLYTKGFLHAKCYLFYSGGGFARFDPVAAIVGSSNFTRPGLLTNKEL
                     NLAHRA"
     gene            complement(913..1467)
                     /locus_tag="VGT44_15595"
     CDS             complement(913..1467)
                     /locus_tag="VGT44_15595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2582278.1"
                     /translation="MSAIPAAPVRLKDGSAITIRTARPRDASAMLALVRPIFAEGEFL
                     LTTLDDFHMTEEREAEWLQMNLDTPGNLVIVAEAEGEMIGMLNFHSQHRRRVAHTGEL
                     GMSVHQAWRDRGVGRALLLVLLAWAEQHPLLEKVCLQVFATNTRAIALDTSLGFQEEG
                     RQVRDIKLESGEYVDVFLMARFVK"
     gene            complement(1510..2652)
                     /locus_tag="VGT44_15600"
     CDS             complement(1510..2652)
                     /locus_tag="VGT44_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2582279.1"
                     /translation="MNCSACGKPLPAGVTYCPNCGAPAALPYSLSGGTPDDPTARASE
                     VTPPPPPFTGYGDQPYSSAPPNPYEMPSSMPYNPYPPAPPTVLPPTVLAPTAQPSSPP
                     PPRGSNRIGLIVGVVILVLLLAGGGLFLALSKGGTTNNPSQQQHTPTVSPAQLTATGQ
                     AQATATVQAALTATAAANATATASVIAANPNPYPPGSGTLALLDPLSNNSLGYQWDIA
                     NNSSGSCAFTGGAYQVSTPKSQFFFLCASNSATYSNFAFEAQVKILKGDCGGLVFRGD
                     LNAGKFYLFEVCQDGSYNLYIFRDYIGNSTLLANGKAAIIKAGLNQSNTLAVTAQGTA
                     LVAYVNSQKVASVTDTSYSHGSIALVTDAYNNPTSVAFSNARLWTL"
     gene            complement(2783..3697)
                     /locus_tag="VGT44_15605"
     CDS             complement(2783..3697)
                     /locus_tag="VGT44_15605"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2582280.1"
                     /translation="MQPITPHLWFDTEAKEAAEFYTSLLPDSQVTNITTLHDTPSGEC
                     DVVSFELAGQPFMAISAGPLFKFNPSVSFFLNFDPSRDTHARAHLDRLWEQLSEGGTA
                     LMPLDTYPFSQHYGWVQDRYGLSWQLMLTNPAGEERPFIIPSLMFTGNMAGHAEEAID
                     FYGAVFKGSRRGLTARYPQGMEPDQEGTLMFADFSILGTWFAAMDSAREHHFTFNEAI
                     SLLVPCETQAEIDYYWEKLSAVPEAEQCGWLKDQFGLSWQVWPTAIGEMMKNGTREQI
                     DRITQAFLPMKKFDLATLQRAYDGMSNL"
     gene            3960..>4269
                     /locus_tag="VGT44_15610"
     CDS             3960..>4269
                     /locus_tag="VGT44_15610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582281.1"
                     /translation="MNQHGAWSPYAGGGAIILAVVLLIVTGVLTYSGIRLRHPLAVKR
                     PGTLLGVTIVVIWLVSVITFLVAITVYVLALYQQAGGHINAPTNPITPVTMVSGAIAF
                     F"
BASE COUNT          916 a         1152 c         1264 g          937 t
ORIGIN      
        1 ggcgcggtgc gccaggttga gttccttgtt ggtcagcagg ccggggcgcg tgaagttgga
       61 cgagccgacg atggctgcca ccgggtcgaa gcgcgcgaag ccgccgccgc tgtagaacag
      121 gtagcacttg gcgtgtaaga agcccttcgt gtagagccgc acctgcacct gcggcctgcg
      181 caggaaggcg atgaggtcct cgacctggcg cagcgtctgc gcgttgaacg gctcctttgc
      241 aagctcatgg atcagtccct tgaccggctt cgcgccgatg tcgcgcaggc caagatcgac
      301 gcccgcctcc ggttcgtcgc cgagcagcag ccggaaggcc cccagcccat ccagcccctc
      361 gcgcagcagt tgccagccgc ccacgttgaa gtaggcagtg gcgatgtcga gcgagtggct
      421 cctgtgctgc gccagcaggt cgttcagcac ggctgccatg ctgtgctgct ggttgtcgat
      481 gacgaacggg gtggtcatgg tttcttcctg tcgtgcgtgt gagatgatgc gctttcccta
      541 tggggggcga gtacgatcat acgcctgttg aaggttgccc gtcaagtagg tgggcaaaga
      601 agccagggca gatgcaaaga cgagaatcaa gcgagtgagg catggcgacc gcaaggggca
      661 tggcgaccgc aagggtgcgc cactacgata acgaggggac tcgtcgtcca aaccgttatc
      721 gtagtggcgc acccttgcgg tcgccatgcc tcgcacgact ttgcatctgc cctgcaatga
      781 agcaagccga tgtgtcactt gtatctgatg tatcagttgt atccgatgtg tcacttgtga
      841 tagatgtttc gggaagggaa agaagtgcct ggtactgacc ttgactcatc tcattctcct
      901 ggatggttct cctcacttca cgaatctcgc catcagaaac acatccacat attcccctga
      961 ttccagttta atgtctcgca cctggcggcc ttcttcctgg aagccgaggg aggtgtcgag
     1021 ggcgatggcg cgggtattgg tggcgaagac ctgtaggcag accttctcca ggagcgggtg
     1081 ctgttccgcc caggcaagca acaccagcag cagggcgcgt cccacgccgc ggtctcgcca
     1141 cgcctggtgg acgctcatac ccaattcgcc ggtgtgcgcg acacgcctgc gatgctggct
     1201 atgaaagttg agcatgccga tcatctcgcc ctcggcctcc gcgacgatca ccaggttgcc
     1261 gggagtgtcg agattcatct gaagccactc cgcttctcgc tcctccgtca tatgaaaatc
     1321 atcgagcgtg gtcagcaaga actctccctc ggcgaagatg gggcgaacga gcgcgagcat
     1381 cgcagacgca tcccgcggac gcgcggtacg gatggttatc gcgctgccgt ctttgaggcg
     1441 aacgggtgca gcagggatgg cggacatgcc ttacctcagt attcttgctc aacttgttgg
     1501 cgcagcgagt tacagcgtcc acaatcgcgc gttgctaaag gcgaccgacg ttggattgtt
     1561 ataagcgtcc gttaccaggg cgatggagcc atggctgtag ctcgtatcgg tgacgctcgc
     1621 aaccttctga ctgttcacgt atgccacaag ggctgtgccc tgtgctgtga cggctagcgt
     1681 gttcgattga ttcaacccgg ctttgataat cgcggctttg ccgtttgcca gcaaggttga
     1741 gtttccgatg taatcccgga agatgtagag attataggaa ccatcctggc atacttcaaa
     1801 aaggtagaac ttaccagcat tgaggtcacc gcgaaagact aatccgccgc aatcaccctt
     1861 gagaatcttc acctgcgcct cgaacgcgaa attactgtac gtggctgaat tggaggcaca
     1921 taagaaaaag aactgggact ttggggtgct gacctggtag gctcctcccg tgaaggcgca
     1981 cgaaccactg gagttattcg caatatccca ctggtagccc aggctgttat tgctcaaagg
     2041 atcgagaagc gccagcgtcc cactgccggg tggatacggg ttcgggttcg cggcaatgac
     2101 cgaggcggtc gcggtggcat tggctgccgc cgtagcagtc agggcggcct gaaccgtcgc
     2161 ggttgcctgc gcctgtcccg tggcagtgag ttgggcgggt gaaaccgtgg gggtatgctg
     2221 ctgttgagat gggttgttcg tcgttccgcc ttttgatagc gcgagaaaca ggccgccccc
     2281 ggccagcaga aggacgagga tgaccacgcc aacgatcaac ccgatacggt tacttcctcg
     2341 tggaggagga ggtgaagaag gctgcgcggt tggcgcaagc acggttggcg gaagcaccgt
     2401 tggaggcgcg ggaggatagg ggttgtaggg catcgaggag ggcatttcgt agggattagg
     2461 cggcgctgag ctatagggct gatcgccgta gccggtaaag ggcggtggtg gtggcgtcac
     2521 ctctgatgcc ctggcggtag gatcatccgg cgttcccccg ctcaacgagt aggggagcgc
     2581 cgccggcgca ccacagttgg ggcagtaggt cactcctgcc ggtagcggct tcccgcaggc
     2641 gctgcaattc atgggaacat gccctccctt gttcaaaaat aaagcactct gtacgataaa
     2701 cggaaaagaa gatgggtgta ttatatacta cacaaccccg gcattgtaat gtgcgggagc
     2761 gtgggctggc gcacacagac attcagagat tagacattcc gtcataggct ctctgtaagg
     2821 tggcaagatc aaatttcttc atcggcaaga acgcctgcgt gatccgatca atctgctcac
     2881 gcgtgccgtt tttcatcatc tctcctatcg cagttggcca cacctgccag gaaagaccaa
     2941 actggtcttt taaccaaccg cactgttcgg cctcaggaac cgcagaaagt ttttcccagt
     3001 agtaatcgat ttctgcttgc gtttcacagg gcacaaggag agagatggcc tcattgaatg
     3061 taaaatgatg ttctcgcgcg ctgtccatag cggcaaacca ggttcccagg atcgagaagt
     3121 cggcgaacat cagcgttcct tcctgatccg gctccatccc ctgagggtag cgagccgtca
     3181 gccctcgcct ggaacccttg aagaccgcac cataaaaatc aatcgcttcc tcggcatgac
     3241 cggccatgtt cccggtaaac atcagcgacg gaatgatgaa gggccgttct tcccccgccg
     3301 gattcgtgag catgagctgc caggacagtc catagcggtc ctggacccag ccgtagtgtt
     3361 ggctgaacgg gtaggtgtca agcggcatca gagctgtccc gccttccgac aactgctccc
     3421 atagccgatc taaatgcgct cgcgcatggg tgtctcttga tgggtcaaag ttgaggaaaa
     3481 aggaaacgga aggattgaat ttgaaaagcg gcccggcgct gatggccatg aacggctgtc
     3541 cagccagttc gaacgaaacc acatcacact ctcccgaagg agtatcatgc agcgtggtga
     3601 tattcgtcac ttgtgaatcc ggcagcaaag acgtgtaaaa ctcggcagcc tctttcgctt
     3661 ctgtgtcaaa ccatagatgc ggtgtgattg gttgcatgct tttgctcctt catgcttact
     3721 tactctactt ctttctcaag tctaccatat tgaacggatg tgcgcgatcc ttcgctgcgc
     3781 tcaggatgac gggcaagata tccacatgtc tgcaatcgcg ggtcaaccct tgacaaaata
     3841 tattgcgata tgttatacaa actatagcat atcgcaatat tctaacaggt gagtaatgaa
     3901 aaaacgagca agggaaaaga ataaaagctt cttctcgcaa gcagagtatg gaggtaacta
     3961 tgaatcaaca tggagcctgg agtccctatg ctggcggcgg cgccatcata ttggcggtcg
     4021 tcctgttgat cgtcacgggc gttttgacgt actccggcat tcgattacgt catccactgg
     4081 cagttaaaag acctggaacg ttgcttggcg ttacaatcgt agtaatctgg ctggtgtctg
     4141 tgataacgtt cctggttgca attaccgtct atgtgctggc gctgtatcag caggcggggg
     4201 gtcatattaa cgcgccgacg aaccctatta cgcccgtgac aatggtatca ggagcgatag
     4261 cctttttcg
//
LOCUS       DASZAK010000889         4926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_22988, whole genome shotgun sequence.
ACCESSION   DASZAK010000889 DASZAK010000000
VERSION     DASZAK010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22988"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..818
                     /locus_tag="VGT44_15615"
     CDS             135..818
                     /locus_tag="VGT44_15615"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEV2582282.1"
                     /translation="MSSVSRRAGNSVPKKKYNAREEEMTVFFEEKARMSESAMQAIQE
                     RQGRDQAFRQQLYNDPLIALSGYDLNEEEIHRLVLPNFRWLIENKIAGMSYPRSEAAL
                     ALLRERGIRALLSLSEDSPPPDLLAKFGLQAEHLPVADFTAPTIEQVEQAVAAINRFL
                     GEGLPVAVHCGAGLGRTGTILASYLVWQGISAQDAIRTIRDQQPGSVETPEQVALIDQ
                     YEQHQHKEN"
     gene            834..1373
                     /locus_tag="VGT44_15620"
     CDS             834..1373
                     /locus_tag="VGT44_15620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2582283.1"
                     /translation="MRIRRYRLGDIPTLAHIQQAAARVDGVTPLSEEDFEEWFAQPGL
                     QAESNVFLITDDDDDLNEWGQAGTLDGLEGEVVGYTVLQLRRSQRAYHFLCEGAVLPQ
                     FRRQGAGLALLICALNYARIRIFELEHENAGQQAHPVYFEALLPASDAASRSLAAKCE
                     MQPVDEPVLRGMRLYRGEV"
     gene            1400..1885
                     /locus_tag="VGT44_15625"
     CDS             1400..1885
                     /locus_tag="VGT44_15625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582284.1"
                     /translation="MALSDLASIAIIVQGMLFIVSIGLVWYQLRANTKLVKAANTQKL
                     VELSAPFNLRLAQDRALTELWWQATQRFDELDEIDQARYFNLLTWWLMLHENIYHQWR
                     SKLIDEDTYSSWTRDLEYYVKSQQLVNHWPRLNGYFEPSFAEHVTTIIVRLTKEAEVT
                     S"
     gene            complement(1920..2840)
                     /gene="thyX"
                     /locus_tag="VGT44_15630"
     CDS             complement(1920..2840)
                     /gene="thyX"
                     /locus_tag="VGT44_15630"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEV2582285.1"
                     /translation="MSVVTPERLYQEFEGDRHSDFPAIHSPALMSPGNAPYLQRPGVV
                     LLARPTVNVPGLAGFLAGFDESLQFPAYLDDPTSLPDGAQLCKVAGQVCYMSFGPKRT
                     FNAQATRYFDNLKSSGHGSVFEHANFSMLFYGISRSVTHELIRHRAGFGYSQLSQRFV
                     SGRMLRFVERPEYQEDELFHNQFIERIERAAAEYKALTNRLLEMQQAGTKILSAEAKT
                     DLRKKVQQCARSVLPNETEAPIVVTGNARSWRHLIEMRADAHAEIEIRELAVRTFLCL
                     HKTDPILFSDYTVEELPDGTYTVKTEYSKV"
     gene            complement(2930..3139)
                     /locus_tag="VGT44_15635"
     CDS             complement(2930..3139)
                     /locus_tag="VGT44_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2582286.1"
                     /translation="MANKDSDLLTVREVAEQLRVDDTTVRRWIKNGILEAITLPHRGA
                     RQAYRIRRETLDALLAPSEHAANTH"
     gene            complement(3351..4346)
                     /gene="moaA"
                     /locus_tag="VGT44_15640"
     CDS             complement(3351..4346)
                     /gene="moaA"
                     /locus_tag="VGT44_15640"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEV2582287.1"
                     /translation="MEPLVDSYGRRIKSMRISITDKCSFRCTYCMPAEGLPWLKKAEI
                     LSYEEIVRIARVAASIGIEQIRLTGGEPLIRRDIPELISQIRQIDGLRSISLTTNGIL
                     LKQLAGSLAEAGLTRINVSLDSLIREKFAQITRRDQMHRVLEGLEELEKYPSIHPIKV
                     NAVAVKGYSEEEALDFVRLARRKAYVMRWIEFMPLDADQIWNKEDILTGAELKAIIEA
                     EYGPLVPITTGDPSETARRYTFSDGIGEVGFINPVSEPFCAQCDRIRLTADGQLRTCL
                     FATEETDLRAIVRSDAPDAVLAHTIRQAVWHKELKHYIGDKRFKRANRSMSMIGG"
     gene            complement(4450..>4926)
                     /locus_tag="VGT44_15645"
     CDS             complement(4450..>4926)
                     /locus_tag="VGT44_15645"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV2582288.1"
                     /translation="MKVSHGSLYPRLRKLEEDGLITATEQVESASENSRQVRVFAITE
                     EGRKYFHELMMDTSSNPGEYSRLFWQKAGNLDCLHPAEQLELIDHYLNYCQMHQLHLK
                     AVTRNVVESKGHYHAMPLTQLELTLHALRRAASHWQVEAEYAGGLREKVLAKMLVE"
BASE COUNT         1080 a         1267 c         1427 g         1152 t
ORIGIN      
        1 aaacgtgccg gcgttcaaat acaaccagga agtagttgca ggcatggcct gtcgaatgtt
       61 ctggagaagg gtagaatcgg gcaagatgtg gctcctttaa ctgatgattg cgagccgact
      121 ctcgctgcac tgaagtgagc agcgttagcc gacgtgctgg aaacagtgta cctaaaaaga
      181 agtataatgc aagagaagag gaaatgacag tattctttga ggagaaagcg cgcatgtcag
      241 agagtgcgat gcaagctatc caggagcgac agggacgaga tcaggcattc cggcagcaac
      301 tctataacga tccattgatt gcacttagcg gctatgatct taatgaagag gaaatacatc
      361 gcctggtgtt gccaaacttt cgctggctca tcgagaacaa gattgccggt atgtcctatc
      421 ctcgctcaga agctgcgctt gctctgttga gagaaagggg tatacgagca ttattgagtc
      481 tatcagaaga ttctcctcca cctgatctgc tcgccaaatt tggcttacaa gctgagcatc
      541 tcccggttgc cgattttacc gcccctacga tcgaacaggt cgagcaagct gttgctgcca
      601 tcaatcgctt cctcggcgag ggtcttccgg tagctgtaca ttgtggcgca ggacttggcc
      661 gaacgggtac gatcctcgca agctacctgg tctggcaagg catttccgca caggatgcca
      721 tcagaacgat acgcgatcag cagcccggtt ctgtcgagac acctgaacag gtagctttga
      781 ttgatcaata cgagcaacat caacacaaag aaaattagag gagcaaacaa gcaatgcgaa
      841 tccggcgata ccggttgggt gatattccta cactggcgca tatacagcag gcagccgcac
      901 gagttgatgg ggtaacgcct ttgagcgaag aggattttga ggagtggttt gcccagcctg
      961 gattgcaggc cgagtcgaac gtatttttga taaccgatga cgacgacgac ctgaacgaat
     1021 gggggcaggc aggtacgttg gatggtttgg aaggcgaggt cgtaggctat accgttctgc
     1081 aactgcgccg gagccagcgc gcctatcact tcctctgcga aggcgccgtg ctgccgcagt
     1141 ttcgcagaca gggcgcgggg ctagcgctgc tgatttgcgc cttgaactat gcgcgcatcc
     1201 gcatcttcga gcttgaacac gagaatgcag gtcagcaggc gcatcctgtc tactttgagg
     1261 cgctgctccc ggcgagtgat gcggcatcga ggagtttggc ggcgaaatgc gagatgcagc
     1321 ccgttgacga gccggtcttg cgggggatgc ggctgtatcg cggcgaagtg tgatgcgtct
     1381 tgaaacagga gttagtagga tggctttgag cgatctggcg agtattgcga taattgttca
     1441 agggatgctt ttcatcgtct ccattggcct ggtctggtat caattgcgag cgaacacgaa
     1501 actggtcaaa gcagccaaca cccagaagct cgtggagtta tccgcgccct tcaatctccg
     1561 gctggcgcag gatcgcgcgt tgacagaact ctggtggcaa gcaacacagc gctttgatga
     1621 gttagatgag attgaccagg cgcgctactt caacctgttg acgtggtggt taatgttgca
     1681 cgagaacatt taccaccagt ggaggagcaa gttgatagac gaagatacct atagctcctg
     1741 gacgcgcgat ctcgaatatt atgtgaaatc gcagcagctc gtcaaccact ggccgcggct
     1801 gaatgggtat tttgagccgt cgtttgctga gcatgtgacg acgatcattg ttcgcctgac
     1861 aaaagaagcg gaagtcacct cttgaccctc gcggtcgccc ttcagacccg cccctttttt
     1921 tagactttgc tatattcggt cttcacggta taagtgccgt cgggcagttc ttccaccgta
     1981 taatcgctga agaggatcgg atcggttttg tgcaggcaga ggaaggtgcg cacagccagc
     2041 tcgcggatct cgatttcagc atgggcgtcg gcgcgcatct cgatcaaatg cctccacgag
     2101 cgcgcgttgc cggtcacgac aatcggcgct tcggtttcgt tgggcagcac cgagcgggcg
     2161 cactgctgca cttttttgcg cagatcggtt ttcgcctcgg cagacagaat ctttgtcccg
     2221 gcctgctgca tctcgagcag gcgattggtc agcgccttgt attcggcggc ggcgcgttcg
     2281 atgcgctcga taaactggtt atggaataac tcgtcttcct ggtattcagg acgttcgaca
     2341 aaacgcaaca tgcgcccgga gacgaaacgc tggctcagct gtgaataccc gaagccggcg
     2401 cggtgccgga tcagttcgtg ggtgacgctg cgcgagatgc cgtagaacag catgctgaaa
     2461 ttggcgtgtt caaagacaga accatgcccg gacgacttga ggttatcaaa atagcgcgtc
     2521 gcctgggcat tgaaggtgcg cttcgggcca aaactcatat agcagacctg gccggccacc
     2581 ttgcagagct gcgcgccatc gggcaagctg gtgggatcat ccaggtacgc cggaaattgt
     2641 agcgattcgt cgaagcccgc aagaaagccc gccagtcctg gtacattgac ggtcgggcgc
     2701 gccagcagca caacccctgg ccgttgaagg taaggtgcgt tgccgggaga catgagcgca
     2761 ggggagtgaa tggccggaaa atcagaatgc cgatcgccct cgaattcctg gtataatcgt
     2821 tcaggtgtaa ctacagacat attcgcctcc agagaatgta ttgtctacaa ctggcatcag
     2881 tttaacaggt tggaagcgat ctggctacgg gggaaatagc tagttgcagt taatgcgtat
     2941 ttgcggcatg ctcggatggg gcgagcaatg catcgagcgt ttcgcgtcga atgcggtatg
     3001 cctggcgtgc gccacggtga ggaagggtga tcgcctcgag gatgccgttt tttatccagc
     3061 gccgcacggt agtatcatcc acccgtaatt gttcggcaac ttcacggact gtcagtagat
     3121 ccgaatcttt gtttgccacg ttgccctctt tctgcttttc gcctacaaca acgggcagtc
     3181 ctgaagagac gttggactgc acaggttatc tatttgacta taacgaacgt acggcgttat
     3241 agtaactctg ttcttgaaat atagctagta acgcaactgc atgtagaacc tgtgcagata
     3301 aatgatgcag ttcgagtgcg actatgctga taacacagca atcgctggcg ctagccgcca
     3361 atcatcgaca tgctgcggtt tgcgcgcttg aagcgtttgt ccccgatgta gtgtttgagt
     3421 tctttgtgcc acacagcctg gcgaatggtg tgtgccagga cagcatcggg agcatcggag
     3481 cgcacaatgg cgcgcaggtc ggtctcctcg gtggcaaaga ggcaggtgcg tagttgccca
     3541 tcggcagtga ggcggatgcg gtcgcattgg gcgcagaatg gctcgctaac ggggttgata
     3601 aagccgactt cgccaatgcc atcgctgaag gtatagcgtc gggctgtttc ggagggatcg
     3661 cccgttgtaa taggtaccag ggggccatac tcggcttcga tgatcgcttt gagttccgcc
     3721 ccggtcagaa tatcttcttt gttccagatc tgatctgcgt cgagcggcat gaattcgatc
     3781 cagcgcatca cgtatgcctt gcgacgcgcc agtcgcacga aatccagcgc ttcctcttcg
     3841 ctataacctt tcaccgcgac cgcgttgact ttgatgggat gaatcgaggg atatttctcg
     3901 agttcttcca ggccctccag cacgcgatgc atctggtcgc ggcgcgtgat ctgcgcgaat
     3961 ttctcgcgga tgagcgagtc gaggctgacg ttgatgcgtg tcagtccggc ctcagccagc
     4021 gacccggcga gctgtttcaa gaggatgcca ttagtggtga ggctaatgct gcgcaggcca
     4081 tcgatctggc gaatctggga gatcagctct ggaatatcgc gccgtatcag tggttcgccc
     4141 ccagtcaggc gtatttgctc gatgccgatg ctggccgcta cgcgcgcaat ccgcacgatt
     4201 tcctcatagg agagtatttc ggcctttttc agccagggca acccttcggc aggcatacag
     4261 taggtacagc gaaagctgca cttgtcggtt atcgaaatgc gcatgctctt gatgcggcgt
     4321 ccgtagctgt caacgagcgg ttccatggtg actcctttat gcctgcagta agttctgcgt
     4381 tccagcagaa aatcgccagc gtgtatcctt tactatatca tatcgtgaca tgcatctgcc
     4441 acgcagccgt tactcgacca gcatctttgc cagcaccttc tcgcgtaagc cgcccgcgta
     4501 ttccgcttcg acctgccagt ggctcgcggc ccggcgcagg gcatggagcg tcaattcaag
     4561 ctgggtcagg ggcattgcgt gatagtgtcc cttgctttcg acgacatttc tggtgacggc
     4621 tttcaggtga agctggtgca tctggcaata attcaaatag tgatcgatca attctaattg
     4681 ttccgccggg tgcaggcagt ccaggttgcc agccttctgc cagaacagcc gggaatattc
     4741 gccagggttt gaagatgtat ccatcatcaa ttcgtgaaaa tacttccgcc cttcctctgt
     4801 gatggcgaag acgcgtacct gtctgctatt ctcactagcc gactccactt gttccgtagc
     4861 ggtaatcagc ccatcttcct ccagtttgcg caagcggggg tagagcgagc cgtgactgac
     4921 tttcat
//
LOCUS       DASZAK010000890         5685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_521303, whole genome shotgun sequence.
ACCESSION   DASZAK010000890 DASZAK010000000
VERSION     DASZAK010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5685
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521303"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VGT44_15650"
     CDS             complement(<1..491)
                     /locus_tag="VGT44_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582289.1"
                     /translation="MLLLRFLFRNLKGFRLLIVLAIIVTVLQVGCDIAAAFPLKFIPS
                     KVANAGNDPACTFPFLNPILDKFDIPLFDPSLNNPIAGQPALTPGLTPCPISPSDANP
                     ITVTTHHSTYGVIVFSVLMLILFGTIAAVLGYLDLYLAAYIAQNLTARLRNQLFEHLQ
                     RLSL"
     gene            complement(600..2954)
                     /locus_tag="VGT44_15655"
     CDS             complement(600..2954)
                     /locus_tag="VGT44_15655"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEV2582290.1"
                     /translation="MTSFLWRNLKGYRFMVFLAMLMAVAQVVATLFSAFPMKFILDKL
                     TGAKTDPPPFLNPLISIFDNIGRSGSANQAINVHSATGVILFSVALIMVLGIATAILQ
                     YFELLLAARVAVNLTARLRKKLFSHLERLSLDWHGRQKKGDLVQRVTSNIADIEKFVT
                     DGVVDSVTGVLTIVGVVVVMLLVNWQFTVLSTVILPAMIVSILGYTSTIKAANKKASK
                     ASGQVASVATEDIGAITVLKAFTLEEREGLRFQKYVGKNREASLRAGGLQAQFTPIVG
                     ILAAIGTAIITGVGAYVASGQSFNFVLTIAATTVSLGTLSVFLSYLGMFYQPLRNLSK
                     LANLMTAASTGADRIQEVLDAAPEVVESNQPYYGPQQFKGDIVYDNVVFSYSPNRPVL
                     KGINLHIPAGKKVALVGLSGGGKTTLVKLISRFYEIQQGAVRIDGIDNRSIPLGVLRQ
                     NVSMVLQDNVLFEGTIRENIAIGKPGATMEEIIQAAKQAYVHETIMSYEAGYETEVRE
                     QGKNFSGGQRQRLAIARAVLRASPILILDEPTAALDVEAEAEVMNALDKLVVGRTVLM
                     ISHRLSTLGSVDDIIVLKDGQIVEQGSFKDLKRRGGVFANLLDEQNRYNQDRDAGKSI
                     LRSAFAPKDFAPPALPPQPPRTPNLQPSGPNIVVPPMAYPGNGGRPGPSVQPVRGPQP
                     QQQGSNKARVLIEIDGRVVGERRLDKPELTVGRLSANDIQVPSQRVSRLHAKIRWENG
                     TWVIEDAESLNGLIYQGNLVERHIFTNGDRVHVAPTAVIQFSTM"
     gene            complement(2974..4473)
                     /locus_tag="VGT44_15660"
     CDS             complement(2974..4473)
                     /locus_tag="VGT44_15660"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2582291.1"
                     /translation="MSDSQVEAGVSIVDLPGIVAGLFERAQRPLLAEPGKQAGLFVRY
                     LRRKPARGLAVIYAVDEIGRRAADHAHNPHRSVSLTLDEKALDGARIRFHAGEARQAS
                     LEVQASGVLRAEALGLSVQAFPADDGLPELAASFDLSREGPLFTPLQDAARAQLDDDS
                     WYIVGAGVEAVRYKPASRCVVRYRLTLSRDGEQDSQQLTLYGKVYADPQQARDVQALQ
                     LRLYNEQVARLGSVAVGQTLQVPMLPRPLGLLTDLGITFNEAVQPTDPQERAITGTRA
                     MQPHVVYGRGGEVSAITVPTQELRLTAVALARLHTSAVRANEASPRTGAKEAKRARER
                     AALIAGRNAAQAEETQRLAKELAGRLEALQPDIYRPAHGGFKSSQLLFHSGKVYVVDF
                     DGFCLADAALDVGYFLAYLRPSGLWYARPGMREWFDAAAQEFVTTYRQAMLDLGASQA
                     AIDGIIERSRLYEAALIFKIATRRVNRLNSPRTKELSAMLQEIATCLAG"
     gene            complement(4470..5315)
                     /locus_tag="VGT44_15665"
     CDS             complement(4470..5315)
                     /locus_tag="VGT44_15665"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582292.1"
                     /translation="MQICLIMDNPETPHHPVIAPALQELSSKHTVRLLDVRPLTGAEA
                     NAEEARHPAADLYLLKSHAPQALELAHSLEQQGALVVNSWASSVACQDRVLMARRMKE
                     ASLPWPRTDSFESLGALVADESFLAAMSFPVILKSYFSHRGDLVEFVRSREQLQELAR
                     KWSHEPVVLQEYAASDGWDIKMWVIDQHIFAARRRTPLEPDAPKEDHIIPSNELPAEW
                     ARIALEIGRVFNMRLYGVDLLVTEQGPIIVDVNGFPGFRGVTGAVSALVNMVEKLLSE
                     GTVKA"
     gene            complement(5356..>5685)
                     /locus_tag="VGT44_15670"
     CDS             complement(5356..>5685)
                     /locus_tag="VGT44_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582293.1"
                     /translation="RKLALQVGKLFGLDIYGLDVVETPQGPAILDINDFPSFGRVPRA
                     VMRISEYVLHAAKRAEMQRAHRRSRSYKGHSMPASPAITSEKALVSSSMLEEQKQAEN
                     LAECVSE"
BASE COUNT         1164 a         1649 c         1685 g         1187 t
ORIGIN      
        1 agcgataagc gttgcaggtg ttcaaacaac tggttgcgca gcctggccgt cagattttgc
       61 gcgatatagg ccgccaggta gagatccagg taacccagga cggcggcaat cgtgccgaag
      121 aggatcagca tcagcactga aaacacgatc acaccatagg tgctgtgatg ggttgtgaca
      181 gtgataggat tggcatcaga tggtgaaatg gggcacggcg taagtcccgg cgtcaaggcc
      241 ggttggcctg caatagggtt attcagcgat ggatcaaaga ggggaatgtc aaatttatcg
      301 agaatcggat tgagaaacgg gaacgtgcag gccgggtcat tccccgcgtt agctaccttg
      361 ctcggaataa atttaagtgg gaaagcggca gcgatgtcgc aacctacctg taagaccgtc
      421 acgatgattg caagcacaat aagcaggcgg aaacctttga ggttgcgaaa gagaaacctg
      481 agtaataaca taactacacc ttcctgaccg tgcttgatag ataggtgaac tatctatcaa
      541 gcgaggtaga cggcaattct gttgagaatc acgccctccg ggtcaggctg gtgatcgctc
      601 tacatggttg aaaattgtat tactgcggta ggcgctacat gaactcgatc tccattggta
      661 aagatgtgac gctctaccag gttgccctgg tagatcaggc cgttcaaact ctcggcgtcc
      721 tcaatgaccc acgtcccgtt ttcccagcga atcttggcat gtaagcgcga gacgcgctgt
      781 gacgggacct ggatatcgtt ggcggagagg cggccaactg ttagctccgg cttgtccagg
      841 cggcgctcac cgacgactct gccatcgatc tcgatcaata cacgcgcttt gttcgagcct
      901 tgttgctgtg gctgcgggcc tctcaccggt tgcaccgagg gaccgggccg cccgccattg
      961 ccaggatagg ccatcggggg gacgacgata ttcggcccgg atggttgaag gtttggcgtt
     1021 ctgggcggct gcggtggcag ggcaggcgga gcaaagtcct ttggcgcaaa tgccgaacgc
     1081 aggatcgact tgcccgcatc cctatcctgg ttatagcgat tctgctcatc aagcaggttg
     1141 gcaaagacgc cgcctcttct tttcaggtct ttgaacgacc cctgctcgac aatttgtccg
     1201 tccttcaaga cgatgatatc atcaacgctg ccgagcgtac tcaggcggtg cgaaatcatc
     1261 aagacggtgc ggccaaccac cagcttatcc agggcgttca tgacttccgc ctcggcctct
     1321 acgtcgagcg cggcggtcgg ttcgtcgagg atcaggatgg gcgaggcgcg cagcacggcg
     1381 cgcgcaatcg ccaggcgctg gcgctgaccg ccagagaagt tcttgccctg ctcgcgcacc
     1441 tcggtttcgt agccagcctc atagctcatg atggtttcat gtacataggc ttgtttcgct
     1501 gcctggatga tttcctccat cgtcgctcca ggcttgccga ttgcgatgtt ttcgcgaatg
     1561 gtgccttcaa acagcacatt atcttgcaac accatactaa cattctgacg cagtacacca
     1621 agagggatgc tgcggttatc gatgccgtcg atgcgcacag cgccctgctg gatctcgtag
     1681 aagcgcgaga tgagcttgac cagcgtcgtt ttgccgccgc cggaaagtcc gaccagggct
     1741 acctttttcc cggctggaat atgcaggttg atgcctttca gcaccggcct gttgggggaa
     1801 tagctgaaga cgacattgtc gtagacgata tctcccttga actgctgcgg gccataatag
     1861 ggctggttgc tctctaccac ctccggcgcg gcatccagaa cttcctgaat gcggtccgcg
     1921 ccggtcgacg cggcagtcat caagttggcc agcttcgaga ggttgcgtag gggctggtaa
     1981 aacatgccca ggtacgagag gaagaccgag agcgtaccca gagagacggt ggtagcagct
     2041 atggtcaaaa cgaagttgaa ggattgccca ctagcaacgt aggcgcctac accagtaata
     2101 atagccgtgc caatggctgc gagaataccg acgatcggag taaattgcgc ctgtaaaccg
     2161 cccgcgcgca gacttgcttc gcgattcttg ccgacgtatt tctggaagcg cagcccttcg
     2221 cgctcctcga gcgtaaatgc tttgaggacg gtaatcgcgc caatgtcttc tgtcgcgacg
     2281 ctggccacct ggcccgatgc tttcgacgct ttcttattcg ccgccttgat agtactcgtg
     2341 tatcccagga tggagacgat catcgcgggg aggatgacgg tcgaaagaac cgtaaactgc
     2401 cagttcacga ggagcatcac gacgaccacc cccacgatcg tcaatacgcc agtgacagaa
     2461 tcaaccaccc cgtcggtgac gaacttctcg atatccgcga tattgctggt gacgcgctgg
     2521 acgagatcgc ccttcttttg cctgccatgc cagtcgagcg agaggcgctc caggtgcgag
     2581 aaaagtttct tgcgcagccg cgccgtcaga tttaccgcga cgcgagcggc cagtaagagt
     2641 tcaaagtact ggagaatagc ggtggctatc cccagcacca ttatcaacgc caccgagaac
     2701 aggatgaccc ctgtagcaga atggacattg atggcctgat tcgctgaacc actacggcca
     2761 atattgtcaa agatactgat caagggattc aggaatggag gtgggtctgt ttttgcaccg
     2821 gtgagcttat ccaggataaa cttcatcggg aaagcgctaa agagggtcgc aacgacctgt
     2881 gcgacggcca tcaacatggc gagaaacacc atgaatcgat agcctttcag gttacgccaa
     2941 agaaaactgg tcattttgtc tctcctcctt cattcaacca gccaggcagg tagcgatctc
     3001 ttgcaacatg gccgacagtt cttttgtgcg cggactattt agacgattga cacgccgggt
     3061 tgcgattttg aagatgagcg cggcttcgta gagccgggaa cgctcgataa tgccatcgat
     3121 ggcggcctgc gacgcgccga ggtcgagcat ggcctggcga tacgttgtga cgaattcctg
     3181 tgctgcggca tcaaaccact cgcgcattcc ggggcgggcg taccacagcc cgctggggcg
     3241 caggtaggcc aggaaatagc cgacatccag cgcggcatcg gccaggcaga agccgtcgaa
     3301 gtccacaaca tacactttcc cactgtggaa gagcaactgg ctggacttga agccgccgtg
     3361 cgccgggcga tagatgtccg gctggagcgc ctccaggcga ccggccagtt ccttcgcgag
     3421 tcgctgggtc tcttccgcct gcgccgcgtt gcggcccgcg atgagcgcgg cgcgctcgcg
     3481 ggcgcgtttg gcctccttcg ccccggtgcg cggcgaggct tcgttggcgc gcacggcgct
     3541 ggtatgcagg cgcgccaggg cgacggcggt caggcgcagt tcctgggtcg gaacggtgat
     3601 ggcgctgacc tcgcctccgc gcccgtatac aacgtgaggc tgcatggcgc gtgtgccggt
     3661 gatggcgcgt tcctgcgggt cggtgggctg tacagcctcg ttgaaggtaa tgcccaggtc
     3721 ggtgagcagg ccgagcgggc gaggcagcat tggtacctgc aaggtctgtc caaccgctac
     3781 gctgcccaat cgcgccacct gctcattgta gaggcgcagt tgcagagctt gcacatcacg
     3841 cgcctgctgc gggtcggcat agaccttgcc gtagagtgtc aattgctggc tatcctgttc
     3901 accatcgcgc gagagcgtga ggcgataacg cacgacgcag cggctggccg gtttatagcg
     3961 cactgcctcg acacccgctc cgacgatata ccagctgtca tcgtcgagct gcgcgcgcgc
     4021 cgcatcttgt agcggagtga agagcggtcc ctcacggctc aaatcgaagc tcgcagccag
     4081 ttccggcagg ccatcgtcag ccgggaaagc ctgcaccgat agccccagcg cctcagcgcg
     4141 cagcacgcct gaagcctgca cttccagtga cgcctggcgc gcctcgcccg cgtgaaagcg
     4201 gatacgcgcc ccgtcaagtg ccttctcatc cagtgtcagg ctgaccgagc ggtgagggtt
     4261 gtgcgcgtga tcggcagcgc ggcgaccaat ttcatcgacg gcgtagatca cggccagccc
     4321 gcgcgccggt ttgcgccgta ggtagcgcac aaacagccct gcctgtttgc ccggctcggc
     4381 caggagcggt cgctgagcac gctcgaagag gccagcgaca atgcccggca gatcgacaat
     4441 cgagactcct gcttcaacct gtgaatcgct catgctttta ccgttccttc actcaacaat
     4501 ttttccacca tgttgaccag cgcgctgact gcgccggtaa cgccgcgaaa ccctgggaag
     4561 ccgttgacat cgacgatgat tggcccctgt tccgtgacca gcaaatcgac gccatagagc
     4621 cgcatattaa agacgcgccc gatttccagc gcgatgcgcg cccattcagc aggaagctcg
     4681 ttcgatggaa tgatgtgatc ttccttcggc gcatccggct ccagtggggt gcggcggcgc
     4741 gcagcgaaga tatgttgatc gatgacccac attttgatat cccaaccatc gctggcggcg
     4801 tattcctgta agaccaccgg ctcgtgactc cacttcctgg ccagttcctg caattgctcc
     4861 ctgctgcgca cgaactcgac caggtctccc cggtggctaa aataactttt aagaatgacg
     4921 gggaagctca tcgcagccag gaaggactcg tcggccacca gcgcgcccaa cgactcgaag
     4981 ctgtcggtgc gcggccaggg caggcttgcc tccttcatgc gccgggccat caggacgcgg
     5041 tcctggcagg cgaccgacga agcccagcta ttgacgacca gcgcgccctg ctgttccagg
     5101 gaatgcgcca gttccaacgc ctggggcgcg tgcgatttca gcaggtagag atcggcggct
     5161 ggatgcctgg cctcttccgc gttggcctct gcccccgtca atggacgcac atccagcagg
     5221 cgcacggtat gcttgctgct caattcctgt aatgctggcg caataaccgg gtgatgtgga
     5281 gtttccgggt tatccataat caggcaaatc tgcatagggc ttctcctttt gttcgcactc
     5341 tacgtgtttt tctcgctatt cactgacaca ttccgcgagg ttttccgctt gtttttgctc
     5401 ttcaagcata gacgaactca ccagggcttt ttcggacgtg atggctggcg atgccggcat
     5461 tgaatgccct ttgtaactgc gggatcggcg atgggcgcgc tgcatctcgg ctcgtttggc
     5521 cgcgtgtagg acgtactcgg aaatacgcat gacggcgcgc ggcaccctgc cgaagctggg
     5581 aaaatcgttg atgtcgagga ttgccggtcc ttgcggcgtc tccactacgt ccaggccata
     5641 gatgtccagc ccgaagagtt tgcctacctg caaggccagt ttgcg
//
LOCUS       DASZAK010000891         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_554829, whole genome shotgun sequence.
ACCESSION   DASZAK010000891 DASZAK010000000
VERSION     DASZAK010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554829"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            835..1215
                     /gene="sdhC"
                     /locus_tag="VGT44_15675"
     CDS             835..1215
                     /gene="sdhC"
                     /locus_tag="VGT44_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEV2582294.1"
                     /translation="MYKGQSGMWSWLFHRISGLGILLFLLIHIVDISLLGFGPKVYDD
                     GIALFGVGIVRLISTALIGGLLFHAFNGVRIMLIDFWPKGARYQRAMFTTVLVLTIVC
                     FLPMAYFVLGPIFGWATGNMSAGI"
     gene            1252..1668
                     /gene="sdhD"
                     /locus_tag="VGT44_15680"
     CDS             1252..1668
                     /gene="sdhD"
                     /locus_tag="VGT44_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEV2582295.1"
                     /translation="MRISTGYGPRPSGGGFETFSWYFFRVSGIGLIFLALIHVFLNHV
                     STDVSCTSYQLVAIRYSDPFWRVYDWLLLTLALLHGMNGLRIIIDDYVQNRGMRLTLQ
                     STIGLLTLVFFLLGTVTLITFQPVAGSLGPSCIPPS"
     gene            1678..>3253
                     /gene="sdhA"
                     /locus_tag="VGT44_15685"
     CDS             1678..>3253
                     /gene="sdhA"
                     /locus_tag="VGT44_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEV2582296.1"
                     /translation="MYSKFDVVIVGSGGAGLMAAMQLPNASVAVLTKVYPTRSHTGAA
                     QGGVAAALGNVEEDHWKWHMYDTVKGGDYLVDQPAAEILTRDAIDAIYELEHRGLPFN
                     RTDDGRIDQRRFGGHTRNYGEGPVRRACYAADRTGHMLLQTMYQQAIKNQVRFFNEYL
                     ALDLLINDGQTCGVVAMEIRTGEIHTFHAKAVLFATGGYGRAWRVTSNAFACTGDGMA
                     IAYRRGIPLQDMEMYQFHPTGLYKVGVLLSEAARGEGGKLLNGKGEYFMADYMPTLKD
                     LAPRDIVSRCMLQEIHKGNGVDGKDYLYLDLRHLGAKTINEKLPDITDFARNYLGVEP
                     ITEPVPIQPTAHYAMGGIPTDLDARVAIDPQWTPLPGFYAAGEVACVSVHGANRLGTN
                     SLVDLIVFGRRAGKHMASFIAENDLAPLPPDPEAYARDLVARLMSSSGGQSAAAIRST
                     LQNEMDMNVFVEREEKGLRHSLDTLDGLQDAYKNVQLQDKDKKFNTELVEAIELGFLL
                     DCSEATIHGALARTESR"
BASE COUNT          654 a          924 c          907 g          768 t
ORIGIN      
        1 atgacgataa ttttatcccc ctggtgtact gtatggacga cttcccgctg ctcatattca
       61 ccagggcaac catcgatgct gcatttcatt gctctctcct ttgtgttgga cttacccact
      121 tgggtaagag aggttcgtat accgtaataa tgaccagccg cgattgagca gttgtgcaca
      181 ctatatgcaa tggacgccct gtacctgtgc ggccatacag caagcagcac gggccacgtt
      241 tgtgttcaga gtaatcctcc aggatttgcc catgagcaat agctaccagg acttcatcca
      301 gtgtaatctc ctcatcgtcc atctcttttt gagcatgttg cgtaacgcga agagctaatg
      361 tcctggcttg catctgtatc cttgtgagga tcacatccat ttagcttctc tcattatgat
      421 ctttgactaa ttatagtata aaagctacct cctggctagt agattatcga tgagtaagta
      481 acagcctgtt tgcgcatccc ctcgctgtgg attataatta ccttacaacg tcaacaacgc
      541 gaatcgggta cacgcctctc tgatggtatt tctgtttgtg tgcctgtccc cgtgtgtgta
      601 cggcgttgaa actagagcag agagtatcgt tatgatgcca gtgaggttgc ttcccaggaa
      661 gcgttcaccc tccctcgtag ctcagggtac ccggtaagat accccttcgc ctggttcaaa
      721 tgtcattcag gcgagcatgg cggtcagcac gatcggtagt cccctgggta cccacaaggg
      781 ctatccttct tgttaccccc gcgtctcgcc ccaagtgttg attttgaggg cagtatgtac
      841 aaaggtcaat ctggtatgtg gtcctggctc tttcaccgca tttcaggact gggcatcctc
      901 ttatttctct tgatccacat cgtggacatt tccttgctgg gatttggccc gaaggtctac
      961 gacgatggca tcgcgctatt tggcgtcggc attgtgcggc tgatctccac ggcgttgatc
     1021 ggcggcttat tgtttcatgc tttcaatggt gtgcgtatca tgctgatcga tttctggccg
     1081 aagggagcgc gctaccagcg agccatgttt acaaccgtgt tggtcctcac tatcgtgtgc
     1141 tttttgccga tggcctactt cgtactcggc ccgatttttg gctgggcgac gggtaacatg
     1201 tccgcaggca tataatgtga gttgatgtag agcaacaaga ggagcaatcg tatgcgtatt
     1261 tcgactggat acggcccgcg accatcgggc ggaggctttg aaaccttctc gtggtatttt
     1321 tttcgcgtct ccggcatcgg gcttattttt ctcgcgctta ttcacgtatt tttgaatcac
     1381 gtctcaacgg atgtctcctg caccagctat caactggttg ccatacgcta cagcgacccg
     1441 ttctggcgcg tctacgactg gctgctgctg acgctggcgc tgctgcacgg tatgaatggc
     1501 ttgcgtatca tcatcgacga ctatgtacag aatcgcggaa tgcgtctcac gttacaatcg
     1561 accatcgggc tattgacgct ggtgttcttc ctgctcggca cggtgacgct gatcaccttc
     1621 cagccggtcg cggggagcct cggccccagt tgcattcccc caagttaagg agagcatatg
     1681 tactcgaaat ttgatgtggt gatcgtcggc tccggcgggg ccggactgat ggctgccatg
     1741 cagctgccca acgccagcgt cgcggtgctg acgaaggtct accccacgcg ctcgcatacc
     1801 ggcgcggccc agggcggcgt ggctgccgcg ctcggcaacg tggaagaaga tcactggaaa
     1861 tggcacatgt acgataccgt caagggcggg gactacctgg tcgatcagcc tgcggcggaa
     1921 atcctgacgc gcgacgccat cgatgctatc tacgaacttg agcatcgcgg cctgccattc
     1981 aaccgcacag atgatggacg catcgaccag cggcgtttcg gcggccacac gcgcaactac
     2041 ggcgagggtc cggtgcgacg cgcctgctac gccgcagacc gcacgggcca tatgctgctc
     2101 cagaccatgt accagcaggc gatcaagaac caggtgcgct tctttaacga gtacctggcg
     2161 ctcgacctgc tcatcaacga cgggcagacg tgcggcgttg tggcgatgga gattcgcacc
     2221 ggggagattc atacattcca cgccaaggct gtgctattcg ccaccggcgg ttatgggcgc
     2281 gcctggcgcg tcacgagcaa cgccttcgcc tgcaccggcg acggtatggc gatagcttac
     2341 cggcgcggca ttcccctgca agacatggag atgtaccagt tccatcccac cgggctgtac
     2401 aaagtgggcg tgctgctctc cgaggcggcg cgcggtgaag gagggaaact gctcaacggc
     2461 aaaggcgagt acttcatggc cgactacatg ccgacgctca aagatctggc gccgcgcgat
     2521 atcgtctcgc gttgtatgct gcaggagatt cataaaggca acggcgtcga tggcaaagac
     2581 tatctttatc ttgacctgcg tcacctggga gccaaaacca tcaatgaaaa gctgccggat
     2641 atcacggatt tcgcccgcaa ctacctgggt gtcgagccga ttaccgagcc ggtgccaatc
     2701 cagccaacgg cgcattacgc aatgggcggc atccctactg acctcgacgc gcgtgtagca
     2761 atcgatccgc agtggacgcc actgcccggc ttctacgccg caggcgaggt ggcctgtgtc
     2821 tccgtgcatg gcgcgaatcg tctcggcaca aactcgctgg ttgacctgat cgtcttcggg
     2881 cggcgcgccg gcaaacatat ggcgagcttc atcgctgaaa acgatctcgc acccctgccg
     2941 cccgatcccg aggcgtatgc gcgtgatctt gtcgcccgcc tgatgagttc gagcggcggc
     3001 cagtcggcgg cggctattcg ctcgaccctg cagaacgaga tggatatgaa tgtttttgtc
     3061 gaacgcgaag agaagggctt gcgccactcc ctcgatacgc tggatggctt gcaggatgcc
     3121 tacaagaacg tgcaattgca ggacaaagac aaaaagttca ataccgagct ggtcgaggcg
     3181 attgagcttg gcttcctgct cgattgttcg gaggccacca tccacggcgc gctggcgcgc
     3241 accgagagtc gcg
//
LOCUS       DASZAK010000892         1767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_628154, whole genome shotgun sequence.
ACCESSION   DASZAK010000892 DASZAK010000000
VERSION     DASZAK010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628154"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VGT44_15690"
     CDS             complement(<1..865)
                     /locus_tag="VGT44_15690"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2582297.1"
                     /translation="MVVAIRQMTYAQAINDAIRMEMRRDPRVILMGEDVAGGATIDHL
                     QDDEAWGGVLGVTKGLVQEFGRNRVLDTPITEAGFIGAAVGAAATGMRPIAELMFVDF
                     FGVCWDQIFDQGAKLRYMFGGKASCPLVIRTMIGAGMGAAGQHSGCHYSVFTHMPGIK
                     TVVPSTPADAKGLLEAAIRDDDLVVVFENKMLYGMSGEVPDGEYVIPLGKADIKREGT
                     DVTIVAISRMVHQALEAATTLQAQGISAEIVDPRTLSPLDEETILSSVAKTHRLIVVD
                     EDNPRCSVATDI"
     gene            complement(877..>1767)
                     /locus_tag="VGT44_15695"
     CDS             complement(877..>1767)
                     /locus_tag="VGT44_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV2582298.1"
                     /translation="KEFAAGKVPGFVHLYAGEEAVAVGVCSHLSDDDYITSHHRGHGH
                     CIAKGVSLREMVAELLGREAGACRGKGGSMHIADVNKGMLGANGIVGGGFPLAAGAGL
                     TAKFNGRGQVAVCFFGDGAGNQGTFHEGLNLAGIWKLPVIFVAENNGYAETTPVSYHM
                     SCRDIAERAAGYNMPGVTVDGLDVFAVYEAAGEAVARARRGEGPSLIECKTYRYYGHF
                     EGDTILYRTKEEEESYRSRDPISAMRRSIVSRGVASDAELDAIDKKVQDSIDLAWQFA
                     EAAPYPSPEDALTDVYVKYP"
BASE COUNT          395 a          528 c          464 g          380 t
ORIGIN      
        1 cgatgtcggt ggcaacgctg caacgtgggt tatcctcgtc aaccacaatg agccggtgtg
       61 tcttcgccac tgaacttagt attgtttctt catctagtgg cgagagtgtg cgcgggtcaa
      121 caatctcggc tgagatgccc tgggcttgca gcgtagtagc tgcctctaaa gcctggtgta
      181 ccatgcggga aatagccacg atcgtgacat ctgtaccttc gcgcttgata tcagccttgc
      241 ccagggggat gacatactcg ccatccggca cctcacctga catgccatag agcattttat
      301 tttcaaagac cacgacgaga tcatcatcgc ggatggccgc ctccagcagg ccctttgcat
      361 ctgcgggggt gctgggaaca actgtcttga tgccaggcat gtgggtaaag actgagtagt
      421 ggcaaccgga atgttggccg gccgcgccca tgccagcgcc gatcatcgtg cggatgacga
      481 gcgggcagct tgccttgcca ccaaacatgt agcgcaactt ggctccctgg tcaaagatct
      541 gatcccagca gacaccaaag aagtctacaa acatcagctc agctatcggg cgcataccgg
      601 tggccgcagc gccgaccgcg gcgccgatga acccggcttc agtgatgggc gtatccagga
      661 cgcgattgcg cccaaactcc tggacgaggc ccttggtgac gccgagtaca ccgccccagg
      721 cttcgtcgtc ttgcaagtgg tcgatagttg ctccgcccgc gacatcctca cccattaaaa
      781 taacacgggg atcacggcgc atttccatgc gtattgcatc gttgattgcc tgagcatagg
      841 tcatttgacg aattgcgaca accattgtct cctcctttac gggtacttga catatacatc
      901 ggtcaacgca tcttctggcg acgggtaggg cgcggcttcg gcaaattgcc aggcaaggtc
      961 gatagaatcc tggacctttt tgtcgatagc atccagttct gcatcactag caacaccgcg
     1021 acttacaatg gagcggcgca tagccgaaat cggatcgcga gagcgatagg actcttcctc
     1081 ttccttggtg cgatataaga tggtatcgcc ttcaaagtga ccgtaatagc gataggtctt
     1141 acattcgatc aagctgggcc cctcaccacg cctggcacgc gcaaccgcct cacccgcagc
     1201 ttcatagacc gcaaagacat ccagcccatc gacggtgaca cctggcatgt tgtagcccgc
     1261 ggcgcgttca gcgatatcac ggcagctcat gtgataggag acaggagtgg tttcagcata
     1321 gccattattt tcggcaacga aaatgaccgg aagtttccag atgccggcaa gattgagtcc
     1381 ttcatggaag gtaccctgat tgccagcgcc gtcaccaaag aaacagaccg cgacctggcc
     1441 tctgccattg aacttagcgg tcagccctgc tcctgccgcg agggggaatc ccccaccaac
     1501 aatgccattt gcgccgagca tacccttgtt cacatctgca atatgcatcg agccgccttt
     1561 gcctcggcag gctcctgcct cgcggcccag cagttctgcc accatctcgc gcaagctgac
     1621 gcctttagca atgcagtggc catgaccacg gtgatggctg gttatgtagt catcatcgct
     1681 cagatgcgag cagacaccta cagcaaccgc ttcttcgcca gcgtacagat gcacgaaacc
     1741 aggaacctta cccgcagcga attcttt
//
LOCUS       DASZAK010000893         2865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_628179, whole genome shotgun sequence.
ACCESSION   DASZAK010000893 DASZAK010000000
VERSION     DASZAK010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628179"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VGT44_15700"
     CDS             <1..1008
                     /locus_tag="VGT44_15700"
                     /inference="COORDINATES: protein motif:HMM:NF024352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HEV2582299.1"
                     /translation="TGRGVELSKTGLVMACPNCGIWRPIPHKQKNGTYKEVRCTNPAC
                     GQIIRYEQAPSAKIIVSAPSSHHKQPIIVGEGINRYQVVETRFIDISKGRSFPRCPNA
                     SARNPKARCAFCDFAALPFVPGEVRVCKQCGQKYSEEDVEDWVYLGINYKSPDLFAGE
                     KMLVRKTGRGIYATIDRTGAYTNQVVFIFKMRPDRPERYEKLRLSYMLGVLNSRMMLY
                     RYYKALGDIEWKSFPYMTQKTIMDLPIRDIDFTDARQTYFHDQIADLVDNIIASSRSP
                     HHVADEQVERFVRELYGVNTPAANNRIESELERIRGFGSLLGSSTDIVDVEDEDDDSP
                     S"
     gene            complement(1005..2720)
                     /locus_tag="VGT44_15705"
     CDS             complement(1005..2720)
                     /locus_tag="VGT44_15705"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEV2582300.1"
                     /translation="MLPLLPLRHKRVNGLAQALMVGLIIGIMLITSGCGGSQQSQQQA
                     TQSRSQLDAFLSHARSIGVPAADLAALVAQEQRLNSTSAPFTLFDDTSATNYYLNQAS
                     QYNQLLTQAQNVVSTVTRADSMQAQIDMQRFQEALAKAQTLKVGNIPAFTQAYSHDQN
                     LLSLAQSPKDYTTISADANQVSSALNSLISTQKVLANFYTTIQQMQAARLDVTAMLAQ
                     YAADMHTLNTITAPSDFDKLRQVVDAQYQMAVVNSIQALPYVGNARLNEFKAQIALLK
                     SYGIDTSSYQQLYNADAQQMRAAKTIAQYLVVSQKIDADIASMQGDLTQGAASSLIGK
                     LDSEANAWGQAHLYHDTFDGNNYILDAGYTMVGIGYWLQQDLYNAYTPADYQAVVAEE
                     QNEFFNFQMLQQDYSDKTPYNQVHQTDLKLMQHYPNLQHGTVLMVSLVEQAMRVYQDG
                     KLINAFYVTTGRVELPSLPGYWSVVNRAAPTKFISSDPPGSPYWYPPTPINYAIEYHS
                     DGYFVHDAWWRVNFGPGTQFPHYDTGGDESFAGNGSHGCINMQESQAAWVYSHTDYNA
                     QIMVY"
BASE COUNT          574 a          718 c          866 g          707 t
ORIGIN      
        1 accggtcgag gggttgaatt gtccaaaacc ggtttggtga tggcgtgtcc aaattgcggt
       61 atctggcggc ctattcccca taaacagaag aatggtacgt ataaggaagt caggtgtacc
      121 aatccggcct gtgggcaaat cattcgctat gagcaggctc cgagcgcgaa gattatcgta
      181 tctgctcctt cctcacacca taagcagcca atcattgtag gcgaaggcat caaccggtat
      241 caagtggtag agacgcgctt catcgatatc tctaaaggca ggtcatttcc ccgctgtcca
      301 aacgcgagtg cgcgcaatcc caaagcgcgc tgcgcatttt gcgattttgc ggcacttcca
      361 tttgtgccgg gtgaagtgcg cgtttgtaag caatgtggcc agaaatatag cgaagaggac
      421 gtagaggatt gggtgtacct cggtattaac tataagtctc ctgacttatt tgcaggtgag
      481 aagatgttag tacgcaaaac tggcaggggt atctatgcta caattgatcg gaccggagcg
      541 tatacgaacc aggttgtctt tattttcaag atgcgtcctg atcgaccgga gcgttatgaa
      601 aaattgcgtt taagctacat gctgggcgta ctcaactcac gcatgatgct gtatcgctac
      661 tacaaagcgc tgggtgatat tgagtggaag agttttccct acatgacaca gaaaaccatc
      721 atggacttac ccatacgtga tatagatttt actgatgctc gtcaaaccta tttccatgac
      781 cagattgccg acctggttga caacatcatt gcaagtagcc gatcgcctca tcatgttgct
      841 gatgaacagg ttgaacgatt cgtgcgcgag ttgtacggag taaacactcc cgcagcaaat
      901 aaccgtatcg aatctgaatt ggagcgcatc agggggtttg gttcgcttct cggatcttct
      961 acggatattg tggatgttga ggatgaagac gacgactccc cctcctaata caccatgatc
     1021 tgcgcgttat aatccgtgtg gctgtagacc caggcggcct ggctttcttg catattgatg
     1081 cagccgtggc tgccgttgcc ggcgaaggat tcgtcaccgc cggtatcata gtgggggaac
     1141 tgcgtgccgg ggccgaagtt gacgcgccac caggcgtcat gcacgaagta gccatcgctg
     1201 tggtattcga tggcgtagtt gatgggtgtg ggagggtacc agtaaggcga gccgggggga
     1261 tcgcttgaga tgaacttcgt cggcgcggcg cggttcacga ccgaccagta gcccggcagc
     1321 gaggggagtt ccacgcggcc tgtcgtgacg tagaaggcgt tgatgagctt gccgtcctgg
     1381 tagacgcgca tggcctgctc gaccagcgag accatcagca cggtgccgtg ctggaggttg
     1441 ggatagtgct gcatgagttt caggtcggtc tggtgaacct ggttgtaggg cgttttgtcg
     1501 ctgtagtcct gctgcaacat ctggaagttg aagaactcgt tctgctcctc tgccaccact
     1561 gcctggtaat cggcgggcgt gtaggcgttg tagaggtcct gttgcagcca gtagccaatg
     1621 ccgaccatgg tgtacccggc gtccaggatg tagttgttgc cgtcgaatgt atcgtggtag
     1681 aggtgtgcct gtccccaggc gttcgcctcg ctgtcgagct tgccgatcag cgaactggcc
     1741 gcgccctgtg tcaggtcgcc ctgcatcgat gcgatgtccg cgtcgatttt ctgcgagacc
     1801 accaggtatt gcgcgatggt cttcgccgca cgcatctgct gcgcatcggc gttatacaat
     1861 tgctggtagc tcgaggtgtc gatgccgtag gatttcagca gagcgatctg cgctttgaac
     1921 tcgttcagcc gggcattccc cacgtatggc agggcttgaa tagaattcac caccgccatc
     1981 tggtactgcg catcgaccac ctggcggagc ttatcaaagt cgctcggagc ggtgatggtg
     2041 ttgagcgtat gcatgtcggc ggcgtactgc gccagcatcg ccgtgacatc caggcgggcc
     2101 gcctgcatct gctggatggt ggtgtagaag ttggccagca ctttctgcgt tgagatgagc
     2161 gagttcagcg cgctggaaac ctggttggca tcggcgctga tggtggtgta gtctttaggc
     2221 gattgcgcaa gggagagcag attctgatcg tggctatatg cttgcgtaaa ggccggtata
     2281 ttgcctactt tgagggtctg cgccttagcg agcgcctcct ggaagcgctg catgtcgatc
     2341 tgcgcctgca tactatcggc ccgcgtgacc gtgctgacga cgttctgcgc ctgcgtcaaa
     2401 agctgattgt actggctggc ctggttcagg tagtagttcg tcgcggaagt atcatcgaag
     2461 agtgtaaagg gggcgctcgt gctgttcaag cgctgctcct gcgccacaag cgcggcaaga
     2521 tcggcggcag gtacaccgat gctcctggcg tggctgagga acgcatcgag ctgtgacctg
     2581 ctctgggtgg cctgctgctg cgattgctgg cttcctccac agccgcttgt gatgagcatg
     2641 atgccaatga tcagaccaac catcaacgcc tgggcgaggc cattgacacg cttatggcgt
     2701 agaggcagca ggggcagcat aggcaattat ctcctatttt tggctcgttg tttttgtttc
     2761 tgtttacgtt gttctttacg gatggccttg ccggttttct tgccggttcc agccgggcgc
     2821 ggcagcgaag gcatgttcat tccagcgata ccgggttgtc catcc
//
LOCUS       DASZAK010000894         3294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_235866, whole genome shotgun sequence.
ACCESSION   DASZAK010000894 DASZAK010000000
VERSION     DASZAK010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235866"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..937
                     /locus_tag="VGT44_15710"
     CDS             <1..937
                     /locus_tag="VGT44_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2582301.1"
                     /translation="MFHVHAWGMPYIATAMGARQVYPGRYAPDMLLALIQREHVTFSH
                     CVPTILHMLLISPASQEVDLSGWKVVIGGSALPKGLAKMAMNRGIDIFGGYGMSETAP
                     VMSIAQLKPGLDELDEDAQLDYRTKAGIPIQLVDLRIVDDQMRDVPHDGKSSGEVVAR
                     APWLTQGYLNDPVNSENLWRGGYLHTNDIGTIDPEGYLLITDRIKDVVKSGGEWISSL
                     AIEDIISQYPGVSEVAVIGIPDEKWGERPLALIVLKQESKHEVTEQQVQAHVRAWAER
                     GVISKWAVPDVRFVDYLDKTSVGKLDKKVMRQKYG"
     gene            1049..1432
                     /locus_tag="VGT44_15715"
     CDS             1049..1432
                     /locus_tag="VGT44_15715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582302.1"
                     /translation="MKKRWTLDQAVRRTIEETWAVFSRGASTSNPTLFLARLADLARS
                     NAFLWTEAGLAVQENATRVVSVGQSLSRSLVLATVAADIFVGYTTLAERAKWLPSLVK
                     PRDWELQHQRGANRCWKRPPRSAAF"
     gene            1396..2058
                     /locus_tag="VGT44_15720"
     CDS             1396..2058
                     /locus_tag="VGT44_15720"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEV2582303.1"
                     /translation="MLETATSLGGVLIKACQFASTRPDLLPTTYIRTLAPLQDRMPPR
                     PWPEIEKVITRELGRSPAGAFAEIEREPVAAASIAQVHHARLHDGREVAVKVQYPDIE
                     NLVATDLEVLELIVRAIARLVPSIQLHPIIDYLKETMPLELDFTNDILIYKHPPIETS
                     HKQRHPGSGPHWKIPVKEWPCVLHRIEQGEPLRKVAEDYHVSYEAIRRVIRAAHQTKQ
                     AG"
     gene            complement(2665..3156)
                     /locus_tag="VGT44_15725"
     CDS             complement(2665..3156)
                     /locus_tag="VGT44_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2582304.1"
                     /translation="MAEQQTNLLPFYKGWDAYQALLIKAIAPLSSEQLALRVAPHLRS
                     IGENVAHIISGRVGNFHLLMGEGDAELAPLEEWDLPSASSRSAVELVGGLEATWQMIY
                     TALVRWAPADLDDVFVEVQNEKPSSFTRQSIIWSTIKHDLHHGGEVSFTLGTHHFVPS
                     LLK"
BASE COUNT          746 a          882 c          939 g          727 t
ORIGIN      
        1 gatgttccat gtgcatgcct ggggaatgcc ctatatcgcc acagccatgg gcgccaggca
       61 ggtctacccg ggccgctacg cgccggacat gctgctggcg ctgatccagc gggaacacgt
      121 gacgttctcg cattgcgtgc cgaccatttt gcacatgctg ctgataagcc cggcgagcca
      181 ggaagtagac ctgtccgggt ggaaggtggt catcggcggt tcagccctgc cgaagggact
      241 ggcgaagatg gctatgaacc gaggcatcga catattcggc ggctatggca tgtcggagac
      301 ggccccggtc atgtcgattg cgcaactgaa acccggcctg gacgaactgg acgaggacgc
      361 gcagctcgac tatcgcacga aggctggcat ccccatccag cttgttgatc tgcgcatcgt
      421 cgatgatcag atgcgtgatg tcccccacga tggcaagtcg agcggggaag ttgtcgcgcg
      481 cgccccctgg cttacccagg gctatctgaa cgacccggtc aattcagaga acttgtggcg
      541 cggcggctac ctgcacacca acgatatcgg gacgatagat cccgaagggt atctattgat
      601 taccgaccgc atcaaagatg ttgtcaagag cggcggtgag tggatctcct cgctggcgat
      661 cgaagacatc atttcccagt atccgggggt gagtgaggtg gcggtcatcg gcataccaga
      721 cgagaaatgg ggggagcgtc cgctggccct gatcgttttg aagcaggaga gcaaacacga
      781 ggtcacggaa cagcaggtcc aggcgcatgt cagagcctgg gcagagcggg gagtcatctc
      841 aaaatgggcg gtgccagacg tgcgcttcgt ggactacctg gacaagacaa gtgttggcaa
      901 gctggacaag aaggtcatgc gccagaaata tggctagacg gtgtggctga gatggtcaca
      961 acaaggcctg tctcctgccc tcacccaagg ccgacctttt tgaaacgagc gaagaaagtg
     1021 gtaagtcgcg atggagaaga gacgaacaat gaagaagaga tggaccctcg atcaggcggt
     1081 gaggcgcacc atcgaggaga catgggctgt ctttagcagg ggggccagca cgtccaaccc
     1141 taccctgttt cttgcccgcc tggccgacct ggcgcgttcc aacgcgtttc tctggacgga
     1201 ggccgggctt gctgtgcagg aaaatgccac gcgcgtggtg tcggtcggcc agtccctctc
     1261 gcgctcgctg gtactggcga ccgtcgcggc agatatcttc gtcggctata ccactctcgc
     1321 tgaacgcgcg aagtggctgc catccctggt caaaccacga gattgggagt tgcagcacca
     1381 gcgcggcgcg aaccggtgct ggaaacggcc acctcgctcg gcggcgttct gattaaggct
     1441 tgccagttcg cctctacgcg gcccgattta ctgccgacca cctatatccg caccctggcg
     1501 cccttgcagg atcgcatgcc gccgcgcccc tggcccgaga tagagaaggt catcacacgg
     1561 gaattgggcc ggtccccggc gggggcgttc gcggagatcg agcgcgagcc ggtggcggcc
     1621 gcctcgattg cccaggtaca ccacgcgcgg ttgcatgatg ggagagaggt ggcggtcaag
     1681 gtgcagtacc cggatatcga gaacctggtg gcgacggacc tggaggtgct ggaactgatc
     1741 gtcagggcta tcgcgcggct ggtgccctcc atccagctcc atccgattat cgactacctg
     1801 aaagagacga tgccgctcga actcgacttc accaatgata ttctgatata caaacaccca
     1861 cccatcgaaa cctctcacaa gcaacgccac ccaggttcag gcccacattg gaaaataccc
     1921 gttaaagagt ggccttgtgt tcttcatcga attgaacaag gcgaaccact cagaaaagtc
     1981 gcagaagatt atcacgtctc ctatgaagca atacgtcgtg ttattcgagc agctcatcaa
     2041 acaaagcaag cggggtaggt gttatttatt cggagtgcag acgaaaatgc ggaaacttac
     2101 cgcagagcca caatttacag ttctatccta cgtttacgct cacaccttga gcgctcatga
     2161 ttaacaaata gctcgatgtg tacgttcata tcaacggaac tcataccatc aacgttattg
     2221 aatgagcaca aattttgcag ttatatggta catcgtactt tttcgttaaa atcgcaccga
     2281 caggccgtat cagaaacttt cgggaaaagc acccctggcg gcttttttct ctctggttcg
     2341 agcgctttgt atctctcggt ttgcggagct ggtgcgtaaa acagggtgtc gatgaccggg
     2401 tttggataaa ttgtcgatat cctttcccca caaggtcgtt taagataaac gtgatctcaa
     2461 cagtaaattg tgggtaatcg ggacctgcgt tcacttgctg gatcagatcg taatcagtcg
     2521 aggaggtttg cagaaactgc tgtgtagcca tctcttggat gtacctccgc caaacggttt
     2581 ctggattctg tttcttctta ttttcgggat ctgcttggga aggttgcgga gttccttctg
     2641 tcccatccct gcttatcctt gtgtctattt tagcagggac gggacaaaat ggtgtgtccc
     2701 cagtgtgaaa gatacctcgc cgccgtggtg gaggtcatgt ttgatggtgc tccagataat
     2761 cgattgacgt gtgaagctag acggtttctc gttctgcact tctacgaaaa cgtcgtccag
     2821 gtcggcaggt gcccagcgaa cgagagccgt ataaatcatc tgccaggtgg cctcaagccc
     2881 acctaccaac tcgacggcac ttctcgacga tgcgctcggg aggtcccatt cttccaatgg
     2941 agccaactca gcatctcctt cacccatgag caggtggaaa ttgccgacac ggccgctaat
     3001 gatatgggcc acattttcac ctatagagcg tagatgtgga gcgactcgca gagcaagttg
     3061 ttcggacgag agtggtgcaa tagctttgat caagagagcc tgataggcat cccacccttt
     3121 gtagaaaggc aaaagatttg tttgctgttc ggccattgag agcatcgttt cctatgcatg
     3181 tgcgtgcaac gacatattct ctcctgaaag aatatcacga aaagcggtca aatctgttcc
     3241 cgcattttgt tcttgtatgg gcatgccttg aaaggcactt cctcactaac tgag
//
LOCUS       DASZAK010000895         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_698117, whole genome shotgun sequence.
ACCESSION   DASZAK010000895 DASZAK010000000
VERSION     DASZAK010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698117"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..852
                     /locus_tag="VGT44_15730"
     CDS             49..852
                     /locus_tag="VGT44_15730"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEV2582305.1"
                     /translation="MFRKLTAIEIAEGALLADIGIVFQLLALYLPIGKSIFQLLTPIV
                     FTIIVLRRDFYVGLMSLVVAVLTVGIISGPGNVTLMLLEAGAGLFLGLVMKHRRSDLF
                     VIFVGTTSGTFAFYALILLSDLALGIPISDLVKGLQHTFSRGTALLGLVASSVGLGPF
                     WQHSLLPPLNAIATWAFTYWWLSYYLFTWVLMLPVVIVVFYLTNLFARMLGYDVKPFP
                     SGWLDALQYWFLRTCVSLIPRNGIGKHWLAQVLRREVRRIGIARQRTKS"
     gene            849..>1538
                     /locus_tag="VGT44_15735"
     CDS             849..>1538
                     /locus_tag="VGT44_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2582306.1"
                     /translation="MMQLPLIDIHHVSYAYAGRTTAQGETQRVLALRDVTLQAWQGDY
                     IALLGHNGSGKSTLAQHCNALLLPGEGKVYVDGYDTADTRQRRAIRETVGMIFQNPDN
                     QIIATVVEDDVAWALTVRGLPAAEIRERVAWAMEAVGITHLRGLPPNKLSGGQRQRLA
                     IAGVLALRPRCIVADEATSQLDPRSRQEIVDLLHHLNQAYGLTILHITHLLEEAALAQ
                     RIVVMEQGAIVM"
BASE COUNT          301 a          441 c          462 g          334 t
ORIGIN      
        1 acaatgcagc agagtcggat gacctggaga aggacagaaa gaacctgcat gtttcgcaaa
       61 ttgacggcta ttgaaatcgc ggaaggggcg ctcctggccg atatcggcat agttttccag
      121 ttgctcgcgc tctatctacc gattggaaaa tcgatcttcc agcttctgac accaatcgtc
      181 ttcacgatca tcgtgttacg ccgcgatttc tacgtcggct tgatgagcct ggtcgtggcc
      241 gtgctcaccg tcggtatcat cagcgggccg ggaaatgtga cgctgatgct gctggaagcg
      301 ggcgcgggac tgttcctggg gttggtgatg aagcatcgca ggagcgacct ctttgttatt
      361 ttcgttggca caaccagcgg cacctttgct ttttatgcgc tgatcctgtt atcagacctc
      421 gccctgggca taccgataag cgatcttgtc aaaggtctcc agcacacgtt ttcgcgggga
      481 accgcgctgc ttggtcttgt cgcatcaagc gtcgggctag gcccgttctg gcagcacagc
      541 ctgctccccc cgctcaacgc tatcgcgacg tgggcgttta cctactggtg gctttcgtac
      601 tacctgttca cctgggtcct catgctgccg gtcgtgattg tggtatttta cctgacgaac
      661 ctgttcgcgc gcatgttggg atatgacgtg aaaccatttc cgagcggctg gcttgacgcg
      721 ctgcaatact ggtttctgcg cacatgtgtc agcctgatac cgcgtaatgg catcgggaag
      781 cactggttgg cgcaagtgct gcgcagagag gtgcgcagga tagggatcgc gaggcagcga
      841 acaaaatcat gatgcaactt cccttgatcg atatacacca cgtcagctat gcctatgccg
      901 gtcgcaccac tgcgcagggt gagacgcaac gtgtgctggc gctgcgcgat gtcaccttgc
      961 aggcgtggca gggcgactat atcgcgctgc tcggccacaa tggcagcggc aaatctacgc
     1021 tggcgcagca ctgcaatgcg ctactcttgc ccggtgaggg caaagtctat gtggatgggt
     1081 acgatacggc ggacacgagg cagcggcgcg ccatccgcga aacggtgggc atgatcttcc
     1141 agaatcccga taatcaaatc atcgcgacag tggtggaaga cgacgtagcc tgggcattga
     1201 cggtgcgcgg attacccgcc gctgagatac gcgagcgcgt cgcctgggcg atggaggcgg
     1261 tcggcatcac gcacctgcgc gggctgcctc ccaacaagct ctccggcggc cagcgacagc
     1321 ggctcgctat cgcgggtgtg ctggcgctgc gcccgcgctg catcgtcgcc gacgaagcca
     1381 cctcgcagct tgatccacgc tcacgccagg aaatcgtcga cctgctgcat cacctcaacc
     1441 aggcttacgg cctgacaatc ctgcacatca ctcatttgct tgaagaagcg gcattggcgc
     1501 agcgtatcgt ggtcatggag caaggcgcaa tcgtgatg
//
LOCUS       DASZAK010000896         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_484466, whole genome shotgun sequence.
ACCESSION   DASZAK010000896 DASZAK010000000
VERSION     DASZAK010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484466"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VGT44_15740"
     CDS             complement(<1..619)
                     /locus_tag="VGT44_15740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582307.1"
                     /translation="MPQGIVKAPDTPQPPVLEPQPDSAQQTFSRLNEAAQRIAREDAV
                     PNRRLPRASRLSPFRDISAEITRASTPLPNYARMHTGRNSQEVAIPDSYPGEENQPPQ
                     NPNAGQPGQWERLKQATPGGQGIQEASLPDFWPWFDNDVEEKESEDIWANRTDPLITR
                     HKPNSKEAALIEQEDIRRALAEGVSTGQVPAFKTLRRKSPLRNIFI"
     gene            1208..1615
                     /gene="mraZ"
                     /locus_tag="VGT44_15745"
     CDS             1208..1615
                     /gene="mraZ"
                     /locus_tag="VGT44_15745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEV2582308.1"
                     /translation="MFLGEYAHTIDSKGRMALPARFRQDLGSGAVISKGMGSCLSIYT
                     AERWNAKSDDLTVGKTSAELRDFERRIYPSANIIELDTQGRLVIPANLRAYAKLDSEV
                     TVAGVRDHIEVWDRATWHAYQQQLEEQGENVPF"
     gene            1641..>2656
                     /gene="rsmH"
                     /locus_tag="VGT44_15750"
     CDS             1641..>2656
                     /gene="rsmH"
                     /locus_tag="VGT44_15750"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV2582309.1"
                     /translation="MQTGRPGHVPVLLEEALNYLQPRPGGQYIDGTLGGGGHTEAILA
                     RSAPDGRVLGIDTDAQALERVGARLADLVNSGRLALVKGNFGELARIVRHEADFSSSD
                     KIGILTRVDGILLDLGFSSYQMDDPQRGFSFSADGPLDMRLDQSQELTAAELVNTASE
                     EQLADIFWRYGEENRSRQIAWRITRERARGPITRTEQLARLAAAGVPYKPGAIHPATR
                     VFQALRIAVNQELTQLEAALPQMLDILSTGRTREEAREGREMNEEEQEGGGRMVIISF
                     HSLEDRLVKVFMRREASDCICPPGIPVCVCGHKARLRILTHKPVTPSREEIDNNPRAR
                     SARLR"
BASE COUNT          566 a          701 c          809 g          580 t
ORIGIN      
        1 cgatgaagat gttgcggagc ggcgatttcc ggcgcagggt tttaaacgct ggcacctggc
       61 cggttgagac accttcggcc agggcgcggc ggatatcctc ttgctcgatc agcgctgcct
      121 ctttgctatt tggcttgtgg cgggtaatca ggggatcggt tcgattggcc cagatatctt
      181 cgctctcttt ttcttcgaca tcgttatcaa accagggcca gaaatccggt agagaagctt
      241 cctgaatccc ctgccctcct ggcgttgcct gtttcaaccg ttcccattgc cccggctgcc
      301 cggcgtttgg gttctgtggg ggctggttct cttcaccggg gtacgaatcg gggatagcaa
      361 cttcctgcga gttcctgccg gtatgcatcc tggcgtagtt gggcaacggt gtacttgccc
      421 gcgtaatctc ggctgatata tcgcgaaatg gggagaggcg cgaggcgcga ggcaggcggc
      481 ggttgggtac agcgtcctcc cgcgcgatgc gctgcgccgc ttcgttgagc ctggaaaacg
      541 tttgctgcgc gctgtcgggc tgtggttcca gcaccggagg ctgcggcgta tcgggcgctt
      601 tgacaatccc ctgaggcatc gttttttgct cgataagggc atcacctgaa ttggcgttga
      661 caggtgcctg cgctgttaag ggagccgtaa tgcttcgcga accgcgcgcg tttagcgttg
      721 gatcagtctg gctaaatgcg cgaatagccg atccctggcg aaaggagctt cgcagcgagg
      781 atcggcactc atcacaaaat acggcggttg cgtcacatgg cctcttacaa cgcaaacatt
      841 ggctcataga aaaatactct ttgccattca accagacaag ttcatgtatt aatacaacgt
      901 tgatacctct ctgtttgtca catcaagcag gcagtaaaca gccatttctg gctatgcgcg
      961 ataatcaact actcacatca ctatatatct cgttatatca acacaacgca tttcgtgagt
     1021 tatcaacatg ttttccactg tgtcaccctt gaatctgtgg ataaatccat tacattcctg
     1081 agcggggtaa tggtatactt gagtggggca aagtggggca aagtggtgat ttgatgagaa
     1141 tagtggcatc aaatgaccgg aattggggag cattggaacg atttgagcgt tttgagaagg
     1201 cagtagcatg ttcctgggcg aatacgcaca tacaatcgac tcgaagggac gcatggctct
     1261 cccggcgcgt tttcgccagg acttaggcag cggcgcggtt atcagcaagg gtatgggcag
     1321 ttgcctgtcg atctatacgg cggaacgctg gaacgctaaa tcggacgatc tgacggttgg
     1381 caagaccagc gcggaattgc gcgacttcga gcggcgcatc tatcccagcg ccaacattat
     1441 tgaattggac acgcagggga gactggtcat tccggccaac ctgcgcgcct atgcaaagct
     1501 tgatagcgaa gtcaccgtag cgggtgtgcg cgaccatatc gaagtctggg accgcgccac
     1561 ctggcatgcc tatcaacaac aattagaaga gcaaggggaa aacgttccct tctaacttcc
     1621 ttagaatatc tggatgaact atgcaaacag gacgaccagg ccacgtacct gtactcctgg
     1681 aagaggcgct gaattatctt cagcccagac ccggcggaca gtatatagat ggaacgctcg
     1741 gcggaggtgg ccataccgag gccattctcg cgcgatcggc ccctgatggg cgagtactgg
     1801 gcatcgatac cgacgctcag gcgctggagc gcgtcggcgc gcgtcttgcg gacctggtca
     1861 acagtggacg attggcgctg gtcaagggaa atttcggaga actggcccgt atcgtaaggc
     1921 acgaggccga tttttcgtct tctgacaaga tagggatact gacccgcgtc gatggcatct
     1981 tgctcgacct gggcttttcg tcgtatcaga tggacgaccc gcagcgcggc ttctcgttca
     2041 gcgccgatgg cccactggat atgcgcctgg atcaatcgca ggaactgacc gctgccgagc
     2101 tggtgaatac cgccagcgag gaacaactcg cggacatctt ctggaggtat ggcgaggaga
     2161 atcgctctcg ccagatagcg tggcgcatta cgcgggagcg cgcgcggggg ccgatcacgc
     2221 gcaccgagca actggcgcgg ctggcggcgg ctggcgttcc ttataagccg ggagctattc
     2281 acccggctac gcgcgtcttt caggcgctgc gcatcgccgt caatcaggag ctgacgcaac
     2341 tggaagcggc gctgccgcag atgctggaca ttctcagcac cggcagaacg cgggaggagg
     2401 caagggaggg gagagagatg aatgaagagg agcaggaagg tggcggacga atggttatta
     2461 tctcgtttca ctcgttggag gatcgcctgg taaaggtgtt catgcggcgc gaagccagcg
     2521 actgcatttg cccgccggga atcccggtct gtgtgtgcgg acataaagcg cgcctgcgta
     2581 ttttgacgca taagccggtg actccatcgc gtgaggaaat cgacaacaat ccccgcgctc
     2641 gcagcgccag gctgcg
//
LOCUS       DASZAK010000897         4749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_342308, whole genome shotgun sequence.
ACCESSION   DASZAK010000897 DASZAK010000000
VERSION     DASZAK010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4749
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342308"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..396)
                     /locus_tag="VGT44_15755"
     CDS             complement(223..396)
                     /locus_tag="VGT44_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582310.1"
                     /translation="MAGEGVIIGVALVGASATVDVVFQPEGVDGERGDAAIGAAAQNA
                     VAAGTVGIQILIG"
     gene            409..570
                     /locus_tag="VGT44_15760"
     CDS             409..570
                     /locus_tag="VGT44_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582311.1"
                     /translation="MDSSGTTFYLTDALGSILTSFTNASGSAQVKGEQDYMPYGSCRR
                     RRGPQGNCG"
     gene            1171..4665
                     /locus_tag="VGT44_15765"
     CDS             1171..4665
                     /locus_tag="VGT44_15765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV2582312.1"
                     /translation="MAVSSRAVALPERVSFARIPDIRPMPGLIQIQLDSFEWFKKEGL
                     RELFEEISPIEDFTGKNLKLEFIVPDDPFGAPKYSEDECRDHDATFAAPLTVKARLTN
                     KETGEIIEKDIFMGDFPLMTKQGTFIINGAERVVVSQLVRSPGVYYTVDEDATTGRRL
                     YAAKVIPARGAWLEFETSNKNQLSVKIDRKRKLPVTTLLRAISHLAREQWFSEDLDLS
                     TDQGILNAFRGVDTDPNVHYIQATMDKDAVKKEDEALLELYKKMRPGDPATIDNARSL
                     VKNMFFNARRYDLGSVGRYKLDRKLDLSVPQSQRILTMDDLVNIIRRLIQLNNGIGRK
                     DDIDHLGNRRVRCVGELIQTQFRVGLLRMERVVKERMSIQEPGQATPNALINIRPVVA
                     AMKEFFGGSQLSQFMDQTNALAEIGHKRRLSALGPGGLSRERAGFDVRDVHRSHYGRI
                     CPIETPEGPNIGLIGNLATYGQINPYGFIETPYRKVYKKLKANDRALIGREYRGLAGR
                     EWEELKTPEGVTVLKARERVRVDEKLFKKLAFLGDTEIRIKPFVTNEVDYRTADDEEN
                     FYIAQANAKIDSKGEFTEERVLARYQGEFVMENADSMDYMDVSPRQTVSVATALIPFL
                     EHDDVNRALMGANMQRQAVPLLRPQSPICGTGIERQVAIDSGQVVVSQASGEVVSATA
                     RHITILDDQGELHTHNMRKFVRSNAGTCINQRSIVHKGDYVAAGEVIADGSSTEDGEI
                     ALGQNILVAFMSWEGGNFEDAILISERIVREDLFTSIHIEKYDTDARDTKLGPEEITR
                     DIPNVGEEGLRNLDERGIIYVGAEVGPQDILVGKITPKGETELTAEEKLLRAIFGEKA
                     REVKDTSLRVPHGERGKVVEVKVFSRDSGDELLPGVNQLVRVSIAQKRKIGAGDKMAG
                     RHGNKGVISKVLPIEDMPFLPDGTPVDIILNPLGVPHRMNIGQIMETHLGWAAHELGF
                     KIATPVFDGASEEDIEEYLTRAGLPPTGKVQLFDGRTGEPFDHPITVGYTYMLKLAHL
                     VEDKVHARSTGPYSLITQQPLGGKAQFGGQRFGEMEVWALEAYGAANILQEILTVKSD
                     DVVGRVKTYEAIVKGENIMEPGVPESFKVLVKELQSLGINVEVLNEDEQKIQFVEDTS
                     NDVMPELGINLSGFEGDQ"
BASE COUNT         1063 a         1422 c         1373 g          891 t
ORIGIN      
        1 gggagattct gcaccagagg aatagccgat ccatatgcat gccgagctag cttaatcaat
       61 agcaagggat gggagccttt tgagatgatt aactggctca catgatctga gcgggctata
      121 cgcgagcggc tccgcctgca ccaacgaaag tggccagggc tacaagagca gttacgacgc
      181 ctggggcaat gtcaccagcc tcagctacgg tgatacgcca cctcaaccaa tcaggatctg
      241 tatgcctact gtgccagcgg caaccgcatt ctgcgccgca gcaccaatgg cagcgtcacc
      301 acgctcaccg tctacgcctt cgggctggaa gaccacgtct acagtggcgc tggcgccaac
      361 cagagcaaca cctattatta ctccctcgcc ggccacctgc tcggcgcatt ggacagcagc
      421 ggcaccacct tctacctcac cgatgcgctg ggcagcattc tgaccagctt caccaacgcc
      481 tctggctcgg cccaggtcaa aggcgagcag gattacatgc cctacggttc ttgccgtcga
      541 cgacgaggac cccagggaaa ttgcggctga gctgctggca cccttgttag gcagcgctga
      601 tcaatatgag cagcaaaata ttcctgcgaa aaatgcttga caaggcgttt attggggtgt
      661 ataattgcat cctgccagag tgtgtatacc ggtaacagtg ctgttacggt gagcaggtaa
      721 cagtttgata catacagcac ggacaggaat aacgagacac ggaacgacgc atcatccccc
      781 acatgccaca tcatgttacg ctttatattg tatggccagg ggtaccgcag gaagctacta
      841 aacgatagtc ccgccaccgt ttacgatcaa tcaggacgtt gtgtgtgctt ttgttttcgc
      901 ggaaaaaggc gctgatttgt tgcgagtctc acgagtatta ccagtaacag tagggaatag
      961 gagtaaccgc gtgtgattgc acagtgcggt tacttttctt gtcatagaac gttgatacat
     1021 cacaggcatc atgggcaata atttgttatt gcgttctttt ccgatttctt ttttcaataa
     1081 gattggcgca tggtcatgct cccgcgttga ccacctgcgt ctttcttcgt ttccgctgat
     1141 gggtgaccag gtgagaggag aatcctacca atggcagtaa gcagcagagc cgttgcgctg
     1201 ccggagcgcg tctcgttcgc gcgcattccg gacatacggc caatgccggg tctcattcag
     1261 atccagctcg actcctttga atggtttaag aaagaagggc tacgcgaact ttttgaagaa
     1321 atctccccca ttgaagactt taccgggaag aatctgaagc ttgaatttat cgtcccagat
     1381 gacccttttg gcgctcccaa gtattccgag gacgagtgcc gcgaccacga cgcgacgttt
     1441 gccgcgccgc tgacggtcaa ggcgcgtctg acgaacaaag agacgggcga gatcatcgag
     1501 aaggatatct tcatgggtga tttcccgctc atgaccaagc aggggacgtt cattattaat
     1561 ggcgcggagc gcgtcgtcgt cagccagctc gtgcgctcgc ccggcgtcta ctataccgtc
     1621 gacgaggatg ccaccaccgg tcgcaggctg tatgccgcca aagtcattcc cgcgcgcggc
     1681 gcctggctcg aatttgaaac cagcaacaag aaccagctca gcgtcaagat cgatcgcaag
     1741 cgcaaactgc ccgtcacgac gctgctgcgc gccatctcgc acctggccag ggagcagtgg
     1801 ttcagcgagg acctcgatct gtctaccgac cagggcatct tgaacgcctt ccgtggcgtc
     1861 gataccgacc cgaacgtcca ctacatccag gccacgatgg acaaagatgc cgtcaagaaa
     1921 gaggacgagg cgctgctcga actgtataag aagatgcgcc ccggcgatcc ggccaccatc
     1981 gataacgcgc gctcgctggt caagaacatg ttcttcaacg cgcgccgcta cgacctgggc
     2041 agcgtcgggc gctacaagct agaccgcaag ctcgacctgt cggtgccgca gagccagcgt
     2101 atcctgacca tggacgacct ggtcaacatc attcgccgcc tgatccagtt gaacaacggc
     2161 atcgggcgca aggacgatat cgaccacctg ggcaaccgcc gtgtgcgctg tgtaggcgag
     2221 ctgatccaga cacagttccg cgtcggcctg ctgcgcatgg agcgtgtcgt caaagagcgc
     2281 atgagcatcc aggagccggg ccaggccacg cccaacgcgc tgatcaacat tcgcccggtg
     2341 gtggccgcca tgaaggaatt cttcggcggc agccagttgt cgcagttcat ggaccagacc
     2401 aacgccctgg cggagatcgg ccacaagcgc cgcctctcgg ccctcggccc cggcggtctc
     2461 tcgcgcgagc gcgccggttt cgacgtgcgc gacgtgcatc gctcgcacta tggccgcatc
     2521 tgcccgattg agacgccgga aggcccgaac atcggcctga tcggcaatct ggccacctat
     2581 ggccagatca acccgtatgg cttcatcgag acgccctatc gcaaagtcta caagaaactg
     2641 aaggccaacg atcgcgccct gatcggacgc gaatatcgcg gcctcgccgg gcgcgagtgg
     2701 gaagaactca agacgcctga gggtgttaca gtcctcaagg cgcgcgaacg tgtgcgcgtt
     2761 gatgagaagc tgttcaagaa acttgccttc ctgggcgata cagaaatccg catcaaaccc
     2821 ttcgtcacca acgaggtgga ctatcgcacc gccgatgatg aggagaactt ctatatcgcg
     2881 caggccaacg ccaaaatcga tagcaagggc gaatttaccg aggagcgcgt actggcgcgc
     2941 taccagggcg agtttgtgat ggagaatgcc gacagcatgg actacatgga tgtctctccg
     3001 cgccagaccg tcagcgtcgc taccgccctg attcctttcc tggagcatga cgatgtgaac
     3061 cgcgcgctga tgggcgcgaa tatgcagcgc caggccgtgc cgcttttgcg cccgcaatcg
     3121 cccatctgcg gcaccggcat cgagcgccag gtggccatcg actccggcca ggtggtcgtc
     3181 agtcaggcca gcggcgaagt cgtctccgct accgcgcgcc acatcaccat cctggatgac
     3241 cagggcgagc tgcatacgca taacatgcgc aagttcgtgc ggtcaaacgc gggaacctgc
     3301 atcaaccagc gttccatcgt ccacaagggc gattatgtcg cggcgggtga ggtgatcgcc
     3361 gatggctcgt ccaccgagga cggcgagatc gccctcggcc agaacatcct ggtggccttt
     3421 atgagctggg agggcggcaa cttcgaggac gccatcctca tcagcgagcg gatcgtgcgc
     3481 gaagacctgt tcacttctat tcatatagaa aagtacgaca ccgatgcccg cgataccaag
     3541 ctcggcccgg aggagatcac gcgcgacatc cccaacgtgg gtgaggaggg cctgcgcaac
     3601 ctggacgagc gcggcatcat ctacgtcggc gcggaagtcg gcccgcaaga tatcctggtg
     3661 ggcaagatca cgccgaaagg cgaaaccgag ctgaccgccg aagagaagct gctgcgcgcc
     3721 atctttggtg agaaggcgcg tgaagtcaag gacacctctc tgcgcgtgcc acacggcgag
     3781 cgcggcaagg ttgtcgaagt caaagtcttc tcgcgcgaca gcggagatga actgctgccg
     3841 ggcgtgaacc agctcgtgcg cgtcagtatc gcgcagaagc gcaagatcgg cgcgggcgac
     3901 aagatggccg gacggcacgg caacaagggc gtcatctcga aggtgctgcc tatcgaggat
     3961 atgcccttcc tgcccgatgg tacgcccgtg gatatcattc tgaacccgct cggtgtgccg
     4021 caccgtatga atatcggcca gatcatggag acccacctcg ggtgggccgc gcatgaactc
     4081 ggctttaaga ttgccacgcc cgtcttcgac ggagcctccg aagaggacat cgaagaatac
     4141 ttgacccgcg ccggcctgcc gccgaccggg aaagtgcagc tcttcgacgg gcgcactggc
     4201 gaaccattcg atcacccgat taccgtcggc tacacttata tgctcaagct ggcgcacctc
     4261 gtcgaagaca aggttcacgc ccgctcgacc ggcccgtaca gcctgattac ccagcagccg
     4321 ctgggcggca aggcgcaatt cggcggccag cgtttcggcg aaatggaagt atgggcgctc
     4381 gaagcctatg gcgccgccaa catcttgcag gaaatcctga ccgtcaagtc cgacgacgtg
     4441 gtgggtcgcg tcaagaccta cgaagccatc gtcaagggcg agaacatcat ggagccgggc
     4501 gtccccgaat ccttcaaggt gctggtcaaa gaattgcaaa gcctgggcat taacgttgag
     4561 gtcctcaacg aggatgagca aaaaatccag ttcgtcgagg atacctccaa cgatgtcatg
     4621 cccgaactcg gcatcaacct atccggtttc gagggggatc agtagggtca gggcgaccgc
     4681 aagggtcgcc cctaccctgc aacgataccc gttcctaccc atcatgcgtg gagggtaggg
     4741 gcgaccctt
//
LOCUS       DASZAK010000898         5484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_450691, whole genome shotgun sequence.
ACCESSION   DASZAK010000898 DASZAK010000000
VERSION     DASZAK010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450691"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VGT44_15770"
     CDS             complement(<1..785)
                     /locus_tag="VGT44_15770"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEV2582313.1"
                     /translation="MTTYKLPEQIAFDYTKQHESSSSRRARAGGRGRSNVNGEQSGEI
                     RRRRVQPHQEEVPPSSYAERRSRYHRPQGQQYTVKRTGQRRFDQTGIAAPEGLTRAPR
                     SLPPMRTPIPPRSSRTRKRGWLRRFLGFFSVLVLAIAAISFALFSPNFRVRGVAVMGT
                     TNPTLIHEIGQMNMVGQNIFLLNVASLTDQIDALPPIEAADVQKAWPDQLVITVVERL
                     PVILWQTPQQTYSVDSHGVVIALAGNSSGAEHLMTIVDMRSKGV"
     gene            complement(782..1840)
                     /gene="murB"
                     /locus_tag="VGT44_15775"
     CDS             complement(782..1840)
                     /gene="murB"
                     /locus_tag="VGT44_15775"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV2582314.1"
                     /translation="MTFDIETAYSELQPHFKGRARRNEALSRHCTFGVGGPADVWVSL
                     ETSEELVELVRLCMERRWPLLLVGNGTNTLYADAGARGVVARLALDAYRIEERGDGTA
                     LLVADTGVSWPRLLNDLAGQGWGGLEFGPGIPGTLGGGVITNAGAHNSDLSEVLEWVE
                     VLDARSASLAQNGLSMPILRRYTHTEIELGYRRSRFRTGRDIKFDAQGYPIVPERGLI
                     EPAEIITHLGVRLHRADPGQLKEIIEQHKQHRKQTQPPQKSAGSVFKNPPGDFAGRLI
                     EQAGLKGMTHGKAQISPRHANFIINLGGASAGDVVALIVAARERVRTQFGVTLELEVE
                     LRGEWETSTTNRQQETRS"
     gene            complement(1851..2999)
                     /gene="murG"
                     /locus_tag="VGT44_15780"
     CDS             complement(1851..2999)
                     /gene="murG"
                     /locus_tag="VGT44_15780"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEV2582315.1"
                     /translation="MRVLVSGGGTGGHIYPALAIAADLRAQHQAEILFLGSDDGLEME
                     IVPAAGFRLATVKAGRLRRFVSLQTITGVLRVPLGMVQAIGIVRKFRADVAFTSGGFV
                     SVPAALAARVNGVPLLMHQQDVPPNLSNRLAAPFAKRISVAFADSLPYFSASKTVLLG
                     NPVRQAMLDVLAISPQMVRGQLGFNAQQQMLLVTGGSQGARHLNQAVCRALPALMERC
                     PGLQVLHISGKDLYNETRRLADSMLAETPDEKRAELERRYRLVAYMNEEMPLALQAAD
                     LVLCRSGASTLTELATLGKPSILVPLPPAIGASPQEANAAMFARKEAAAVIKNADLTP
                     ETLVERVQAMLSSATQLAAMAQAVSSFARPQATQDITREIVTIAKIRK"
     gene            complement(3009..4616)
                     /gene="ftsW"
                     /locus_tag="VGT44_15785"
     CDS             complement(3009..4616)
                     /gene="ftsW"
                     /locus_tag="VGT44_15785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HEV2582316.1"
                     /translation="MTADNRYSRNNRRQPEQTRLTQSRHITSPVRSPVLESMERQRAA
                     RRPANAGGRGGRTRRYAAEEAINQDAALAGATGTRKKLPPLRLSMQQEALHTGLTAGW
                     RARARQAKEQEQNQLDAPTQRAPRPAAGLKTGITQEEAARLADLEMQLPRVAGKIDSW
                     LLISVLALLCIGIVMVYSASAFQAARYEGDAAYYFQRELIWMMLGIVAMLLTMRIDYR
                     VWRRYSLPGMIVVLPLLVIVLRFGTSVYGASRWIVLGSFFSIQPSELTKLVLALYIAD
                     WLARKGKQVGTFLYGLLPFVILVGVILGLILLENDLGTTIIIAGFATVMFFTAGANIL
                     QFLLAMASGSVVFVLEAFKGYRYYRLTGFLNPFHDVTGINLQLYQALLAFGSGGWFGL
                     GLGASRQKTGYLPFPFSDSIFPIIGEELGFVACAIIVLLYLFLAFRGFRLARRTRDLY
                     GSLLATGITTWLILQAMINIGANTGSIPYTGVPLPFISFGGSSLIVSLAAVGMLLNIS
                     RYIQEPESPALSRRPFPFRKKISVVGV"
     gene            complement(4678..4986)
                     /locus_tag="VGT44_15790"
     CDS             complement(4678..4986)
                     /locus_tag="VGT44_15790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582317.1"
                     /translation="MQHGKEDQQDYGLVKNIEQQLELSAAWRALLGLEDSPSEPGAAN
                     DAPGAPVLKNDPWSALLQTQGIEAVDLQKLHMQTAQLALDERDVELALNNIQEQQAQS
                     "
     gene            complement(5043..>5484)
                     /locus_tag="VGT44_15795"
     CDS             complement(5043..>5484)
                     /locus_tag="VGT44_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEV2582318.1"
                     /translation="VYQTIANGGVMMQPYLVAAINNNGKIATTQPTVKRRVVSAQAAS
                     LLTGMLEQAAIKGFAQLAQVPGYSIAAKTGTATTQGIATDETEASVAGFIPATNPQFV
                     ILVKLDRPQKAIYGSTAAAPLWGTIAQQLMWYYNIPPDEPQQLG"
BASE COUNT         1046 a         1754 c         1555 g         1129 t
ORIGIN      
        1 actcctttac tacgcatatc gacaattgtc atcaaatgct cagccccgct gctgttaccg
       61 gcaagggcta tcacgacccc atggctgtca acactatagg tttgctgcgg tgtctgccag
      121 agaatcaccg gcagccgctc aacaaccgtg atgacaagct ggtcgggcca ggctttctga
      181 acatccgccg cttcgattgg cggcagcgcg tcgatctggt cggtcagcga ggccacgttg
      241 agtaagaaga tgttctggcc gaccatgttc atctgcccga tctcgtgaat cagtgtagga
      301 ttggtagttc ccatcacagc cacgccgcgc acgcgaaaat tggggctgaa cagcgcgaag
      361 ctgatcgccg cgattgctag cacaaggact gagaagaagc ccaggaaacg ccgcaaccag
      421 cctcgcttgc gcgtccggct gctgcgcggc ggaatgggcg ttcgcatcgg gggcagcgaa
      481 cgtggcgcgc gcgtcagccc ttcaggagcc gctatccccg tctgatcaaa tctgcgctgc
      541 cccgtgcgct tgaccgtgta ttgctgtccc tgcgggcggt gatagcgcga ccggcgctcg
      601 gcatagctcg atggcggcac ctcctcctgg tgcggttgta cacggcgtcg gcgaatctcc
      661 ccagattgct ctccgttcac gttgctcctg cctctcccac ccgctcgcgc gcgtcgcgag
      721 ctggatgatt catgctgctt ggtatagtca aaagcgattt gttcgggtaa tttataggta
      781 gtcatgagcg tgtttcctgt tgcctattgg ttgtgctggt ttcccattcg ccgcgcagtt
      841 cgacttccag ttcgagtgtg accccgaatt gcgtgcgaac ccgctcgcga gctgctacga
      901 ttaacgccac cacgtcaccg gcgctggctc cgcccaggtt gatgatgaag ttggcgtggc
      961 gtggcgaaat ctgcgccttg ccatgcgtca tgcccttcaa acccgcctgt tcgatcagcc
     1021 gtccggcaaa gtcgccgggt gggtttttga agaccgagcc tgcgctcttc tgtggcggct
     1081 gtgtctgttt ccgatgttgc ttatgctgtt cgataatctc ctttaactgc cccgggtccg
     1141 cgcggtgcag ccgcaccccc agatgggtga taatctcggc tggctcaatc agcccgcgct
     1201 ccgggacaat cgggtagccc tgcgcatcga acttgatatc gcgcccggtg cgaaagcgac
     1261 tccgccggta gcccaattct atttcagtat gagtatagcg tcgtaatatc ggcatactca
     1321 gtccattttg cgccagggat gcgctgcgcg cgtccagcac ctcaacccat tccagcacct
     1381 cgctcaaatc actgttgtgc gcgcccgcgt tcgtgatgac gccgccgccc agcgtaccag
     1441 gaatgccagg gccgaactcc aggccgcccc agccctgccc cgcgagatcg ttgagcaggc
     1501 gcggccagct caccccggtg tccgcgacca gcagcgccgt gccgtcgccg cgctcctcga
     1561 tacgatacgc atccagcgcg aggcgagcca cgacaccgcg cgcgcccgca tcggcgtaga
     1621 gtgtgtttgt gccgttaccg accagtagta gtggccagcg gcgctccatg cacaggcgca
     1681 ccagctctac cagttcctcc gacgtttcga gcgaaaccca cacatccgcg ggtccgccca
     1741 caccaaaggt gcaatgccgc gacagagctt cgttccgtcg cgcgcgcccc ttgaagtgcg
     1801 gctgtagctc gctatatgct gtctcgatgt cgaacgtcat acgattaatc ctatttcctt
     1861 atctttgcga tagtgacgat ttcccttgtg atatcctgcg tggcctgcgg cctggcaaag
     1921 ctgctgacgg cctgcgccat cgccgcgagt tgcgtcgcgg atgacagcat cgcctgcact
     1981 cgctctacca gggtctccgg agtcaggtca gcatttttga tgaccgccgc cgcctctttg
     2041 cgagcgaaca tggccgcgtt ggcttcctgc ggcgaagcgc caatggccgg tggcagtgga
     2101 accaggatgc tcggcttgcc cagcgtcgcc agctcggtca gcgtcgacgc gccggagcga
     2161 cagagtacca ggtctgccgc ctgcaacgcc aggggcatct cctcgttcat gtatgccacc
     2221 agccggtagc gtcgctctag ttcagcgcgt ttctcatccg gcgtttccgc cagcatacta
     2281 tccgccagtc tacgagtttc gttatacaaa tccttgccgc tgatgtgcag cacctgcagg
     2341 ccgggacagc gctccatcag cgccgggagc gcgcgacaga ccgcctggtt cagatgcctg
     2401 gccccctggc tgccgcccgt cactagcagc atctgctgct gcgcattgaa gccgagctgg
     2461 ccgcgcacca tctgcggcga aatcgccagc acgtccagca tggcctgccg caccgggttg
     2521 cccagcaaaa cggttttgct ggccgagaag tacggcagcg agtcggcaaa tgctaccgag
     2581 attcgcttcg cgaacggcgc ggctagccgg ttcgacaggt taggcggcac atcctgctgg
     2641 tgcatcaaca acggtactcc gttcactctg gctgccaggg cagccggtac tgatacaaaa
     2701 ccgccgcttg taaaagcgac atccgcacga aacttcctca cgatgccaat agcctgaacc
     2761 atgcccagcg gcactcgcag cacaccggtg atagtctgca gcgaaacgaa acggcgcagc
     2821 ctgcccgcct tgacggtggc caggcgaaat cccgccgccg gtacaatctc catctccaac
     2881 ccatcatcac tgccgagaaa taagatctcg gcctggtgct gcgcccgcaa gtcggcggca
     2941 atcgccaggg ccgggtaaat gtgacccccg gttccgccgc ccgatacaag tactcgcata
     3001 tgctccatct ataccccgac gacgcttatt ttcttccgaa acggaaaggg tcgccttgat
     3061 agggcgggcg actcaggctc ctgaatgtat cgcgagatat tcaaaagcat accgaccgcc
     3121 gccagggaga cgatcagcga ggagcctcca aaactgataa aaggcagcgg cacgcctgtg
     3181 tacgggatcg agccggtgtt tgccccgata ttgatcatgg cctgtaagat cagccaggtc
     3241 gtgatacccg tcgccagcag cgagccgtag aggtcgcgtg tgcgcctcgc caggcggaag
     3301 ccgcgaaacg ccaggaacag gtacaacagc acgataatgg cacaggcgac gaagccgagt
     3361 tcctcgccga tgatcggaaa gatcgaatcg ctgaacggga agggcaggta gcccgtcttt
     3421 tgacggctcg cgcccaatcc cagcccgaac cacccgcctg agccgaacgc cagcagcgcc
     3481 tggtacagtt gcaggttgat gccggtgaca tcgtgaaaag gattgagaaa tcccgtcaga
     3541 cgatagtagc ggtagccttt gaaggcttcc agcacaaaca caacactgcc gctggccatc
     3601 gctagcagaa actgcaggat attcgcgccc gccgtaaaaa acatgaccgt agcgaatccc
     3661 gcgataatga tcgtcgttcc caggtcgttt tctaacagga tcaggcccag gatcacgccg
     3721 accaggatca caaacggcag caagccgtac aggaatgtgc cgacctgctt gcccttgcgc
     3781 gccaaccagt ccgcgatgta aagcgccagt acaagcttgg tcagttcgct cggctgaatc
     3841 gagaagaacg agccaagcac gatccagcgc gatgcgccat acacgcttgt cccgaacctg
     3901 agtacaatga ccagcaacgg cagcacgacg atcataccgg gcagcgagta gcgacgccac
     3961 acgcgatagt cgatgcgcat cgtgagcagc atggcgacga tgcccagcat catccagatc
     4021 agctcgcgct ggaagtagta ggccgcgtca ccttcgtaac gggccgcctg gaacgcgctg
     4081 gcgctgtaca ccatcacgat gccgatacac agcagagcca gtacgctgat gagcagccag
     4141 gagtcgatct tacccgccac gcgcggcaac tgcatctcca ggtctgcgag acgcgcggcc
     4201 tcttcctgcg tgatgcccgt cttcagcccg gcagccgggc gtggggcgcg ctgggtcggc
     4261 gcgtccagtt gattttgctc ttgctccttc gcctgcctcg cgcgggcgcg ccacccggcg
     4321 gtcaggccgg tatgcagcgc ctcctgctgc atactgagtc gcagtggcgg cagctttttg
     4381 cgcgtgcctg tagcgccagc cagggcagca tcctggttga tcgcttcctc cgccgcataa
     4441 cgcctcgtcc tgcctcctct gccgccagca ttcgccgggc gcctggccgc tcgctgccgc
     4501 tccatagact ccagcacggg cgaacgcaca ggcgaggtga tatgcctgct ctgcgtaagc
     4561 ctggtctgct ccggctgtct tcgattgttg cgactatagc gattgtctgc tgtcatgctg
     4621 cttccctgcg gaatctggtt ctctgtcctc ttgacatgga acaaattttc tagtatacta
     4681 agattgcgcc tgttgctcct ggatattgtt cagggcaagt tccacatctc tttcatcaag
     4741 ggcgagttgc gcggtttgca tgtgcaattt ttgcaaatcg actgcctcga taccctgcgt
     4801 ctgcaaaagg gcagaccagg gatcattttt gagaaccggc gcgccaggtg catcgttcgc
     4861 cgcgccaggc tcggaaggag aatcttccag gccaagcagc gcgcgccacg ctgcgctcag
     4921 ctccaattgt tgttcaatat ttttgacaag accatagtct tgttggtctt ccttgccatg
     4981 ttgcatgatc ctatcctttt ccaatacacc ccattcctca tttccaggtc gccccggccc
     5041 tgctacccca attgttgcgg ttcatctggc gggatattgt aataccacat cagttgctgt
     5101 gcgatggttc cccacagcgg cgccgctgct gtgctgccat aaatggcctt ctgtggccgg
     5161 tccagcttca caagaatgac gaactgcgga ttcgtggcgg gaatgaaccc cgcgacggat
     5221 gcctcagtct cgtcggttgc aataccctgc gtcgtggcgg ttccagtttt agcggcgatg
     5281 ctatatccgg gaacctgggc taactgtgca aagcctttga tggccgcttg ctccaacatg
     5341 ccggtcagta acgacgcggc ctgtgcgctg acaacgcgcc gcttgacggt cggctgcgtc
     5401 gtcgcgatct tgccattgtt attgatcgcc gctaccaggt agggctgcat catgacgccc
     5461 ccgttcgcga tcgtctgata gacc
//
LOCUS       DASZAK010000899         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_306650, whole genome shotgun sequence.
ACCESSION   DASZAK010000899 DASZAK010000000
VERSION     DASZAK010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306650"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..846
                     /locus_tag="VGT44_15800"
     CDS             82..846
                     /locus_tag="VGT44_15800"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEV2582319.1"
                     /translation="MYPWLTLGPFTISTYSLCFLAAFIVGGLLTYHEVKRQHRATEAI
                     LRVALGALAGGLVGAKVSMLIFLGPATFIKDLPYLWYSGQAWTGGFFGGYLGVILVKR
                     FNHIKYSTGDLFAVALPLAQAIGRLGNLLGGDPFGLPSNLPWAIVQQGVRRQPSALYE
                     LILDLVLFVVIWRLRDKLPRPGDLFKLYIVGYCSLRFLVDFTRADPRVLLGLTLVQVL
                     YAFAIAGFGCQLFVSFRQSRQAKTIPEPGNQTADVL"
     gene            864..1175
                     /locus_tag="VGT44_15805"
     CDS             864..1175
                     /locus_tag="VGT44_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582320.1"
                     /translation="MAQPNDPERGKQYWLGLGLGFVPLVAFLIFYGLALRSPSSLASP
                     FTSGVIIAFIVWVIELFVVIGFLASGRVRFVGYGLLTAFLVTPIVTAVACNALPHIIH
                     V"
     gene            1193..>2495
                     /locus_tag="VGT44_15810"
     CDS             1193..>2495
                     /locus_tag="VGT44_15810"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2582321.1"
                     /translation="MRKPRVSPPEHTTAPAQADSGAEDVSMARHDRPYIYHNFTNSLC
                     HKCLKVVSAKVILQDNKVYLLKTCPEHGAMRSLLSSDAGYYLSQSQYNKPGTLPRRFQ
                     TPVEKGCPLDCGLCTDHEQHTCLALIEITESCNLRCPTCFADSDHGRYSSLDEVERML
                     DTVVENEGYADVVQISGGEPTIHPQILEILELAKSKRIKALMINTNGVRIARDEDFVR
                     SLSQFKGNFEIYLQFDGFRQSTYEDLRGLDLREVKQQAIANLTRYEIPINLTATIKRG
                     LNEDEIGEIVKFGVAQKMVRGITFQPITNSGRHDDFNPLDRTTVPDVIRAIDEQTGGM
                     FRKTDFVPLPCHSDCISMTYAYVNGRKVKPLPRYIDVKSYLDVIGNQINFKIDDMKGV
                     VGSALMRLWSASMPLSTANALRDFSCCLPVSPQILSQQEHGS"
BASE COUNT          511 a          718 c          686 g          580 t
ORIGIN      
        1 gaggtagagt gttccgtaat attccttcaa caatggcgcg cgatactttt gcttatttat
       61 gagcagaaat ggagttagtc catgtatccg tggcttacac tgggaccctt cactatttcc
      121 acctattctc tgtgtttcct cgctgctttt atcgtaggtg ggctgctgac gtaccacgaa
      181 gtcaaacggc agcaccgcgc cactgaggct attttgcggg ttgcgcttgg agcgctggcg
      241 ggtgggctgg tcggggcgaa agtaagcatg ttgattttcc tgggacccgc gacctttatc
      301 aaggatctgc cctatctctg gtatagcggc caggcgtgga cggggggctt tttcggcggc
      361 tatcttggcg tgatactggt caaacgcttc aatcatatca agtactcaac cggtgatctc
      421 tttgcggtgg cattgccgct ggcgcaggcc atcgggcggc tcggcaatct gctgggcggc
      481 gacccgttcg gcctgcccag caacctgccc tgggccatcg tgcaacaggg tgtgcgccgc
      541 cagccctcgg cgctctacga acttattctc gacctggtac tctttgtcgt gatctggcgc
      601 ttgcgggata agctgccccg tcccggcgat ctgttcaagc tctacatcgt cggctattgc
      661 agcctgcgct tcctggtcga tttcacccgc gccgatccac gagtcctgct cggcctgaca
      721 ctggtgcagg tcctctatgc cttcgctatc gccggttttg gctgccaact ttttgtgtcg
      781 ttccgccaga gtcggcaggc gaagacgata cctgagcctg ggaatcaaac ggctgatgtg
      841 ctgtagcgtg aaggagaacg aacatggcgc aacctaacga tcctgaaaga gggaaacaat
      901 actggctggg tcttggcctg ggctttgtcc cgctggtcgc ctttctcatt ttctacggcc
      961 tcgctctgcg gtcaccatca tccctggcct ctcccttcac gagcggagtg atcatcgcgt
     1021 tcatcgtgtg ggttatcgag ctgttcgtgg tcatcggctt cctggcgagc ggccgggtgc
     1081 gctttgtcgg ctatgggctt ttaacggcgt ttcttgtgac ccctattgtg actgcggtgg
     1141 cctgtaatgc cttgccgcac attattcatg tataaatgag gaggacgaaa ccatgcgcaa
     1201 acccagagta tccccacctg aacacacgac ggctccagca caggcagaca gcggcgcgga
     1261 ggacgtctcg atggcccgtc atgatcgccc ttacatctac cacaacttta ccaacagcct
     1321 ctgccacaaa tgcctgaaag tggtgtcggc gaaggtcatt ttgcaggaca ataaagtcta
     1381 cctgctaaaa acctgtcccg aacatggcgc gatgcgctcg cttttgtctt cagacgcagg
     1441 ctactatcta agccagagcc agtacaacaa gcccggtacg ctgcccaggc gcttccagac
     1501 gcccgtagag aaaggctgcc cgctggactg cggcctctgc accgaccacg agcagcatac
     1561 ctgcctggcg ctgatcgaga ttacagaatc ttgcaacttg cgctgcccca cctgcttcgc
     1621 cgattcggat catggccgct actcatcgct cgacgaggta gagcggatgc ttgatacggt
     1681 cgtcgagaac gagggctatg ccgatgtcgt ccagatcagc ggcggtgaac ccaccatcca
     1741 cccgcaaatc ctggaaattt tggagttggc aaagtcgaag cgtatcaaag ccctcatgat
     1801 taataccaac ggcgtgcgca tcgcgcgcga cgaggatttt gtgcgcagtc taagccagtt
     1861 caaaggcaat tttgagatat atttgcagtt cgacggcttt cggcagagta cctacgaaga
     1921 cctgcgcggc ctggacctgc gcgaagtcaa gcagcaagcc atcgccaatc tgacccgtta
     1981 cgagataccg atcaacctga cggccacaat caagcgggga ttgaacgagg atgaaattgg
     2041 cgagatcgtc aaatttggtg tcgcgcagaa aatggtacgc ggcatcacct tccaaccgat
     2101 caccaattct ggtcgccacg atgacttcaa cccgctcgac cgcacgacgg tacccgatgt
     2161 gatccgcgcg attgatgagc aaaccggcgg catgtttcgc aagaccgatt ttgtgccttt
     2221 gccctgtcac agtgattgta tcagcatgac ctacgcctat gtgaacgggc gcaaggtcaa
     2281 gccgctgccg cgctacatcg atgtgaagtc gtacctggat gtgattggca atcagatcaa
     2341 tttcaagatc gatgatatga agggcgtcgt cggctcggca ttgatgcggc tctggtcagc
     2401 ctcaatgccg ctcagcaccg ccaacgcact gcgcgatttc agttgctgcc tgcccgtatc
     2461 accgcagata ctctcgcaac aggagcatgg cagtt
//
LOCUS       DASZAK010000900         3211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_521505, whole genome shotgun sequence.
ACCESSION   DASZAK010000900 DASZAK010000000
VERSION     DASZAK010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521505"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..1374
                     /locus_tag="VGT44_15815"
     CDS             277..1374
                     /locus_tag="VGT44_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582322.1"
                     /translation="MPILQTSQTGGRGAIQHYEYVFPDGEMDVYDMDHGQALVQRVAL
                     PTFAGVRGVVASPVTHMLYVSYGGDSGDSGTGGSLLKYDLLTQRIVWQRTYAHGIDSM
                     AITPDGKTIYMPDGELSSDGRWYIVDAGSGNETGFIDAGTGPHNTLVSLNGKHVYLGG
                     RHTPYLTVVDTATNQVIQEIGPLKGDGVRPFTINGRETLAYMSTTGFLGFQVGDIQTG
                     KILYTVPIQGFSWDGSGATDPSHGISLSPDEKELYVIDWANSYVHVFDVSHVPASAPR
                     QVADIRLTRSMRHNESPCAYDCLADGWLQHSADGRFVYVGDEGDVIDTASRQVVANLD
                     TLYNTRKMLEIDWRLGVPVFTTSRQGMGYVT"
     gene            1421..1705
                     /locus_tag="VGT44_15820"
     CDS             1421..1705
                     /locus_tag="VGT44_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582323.1"
                     /translation="MPGTSLAKKLVIKPHYRIVLLNAPRGYRELVGELPDGVTVATDG
                     DGLFDMVQAFAEQRLRRLRRWRSRRSSRMDCYGSAIPRRPGRSRRTSRER"
     gene            complement(1882..3066)
                     /locus_tag="VGT44_15825"
     CDS             complement(1882..3066)
                     /locus_tag="VGT44_15825"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEV2582324.1"
                     /translation="MSDQEMHFADPDWQPTQLRASTPPRASMPISPAPPIQQINDAQL
                     SNTPKADFQSASGEKIDTGDDADYTPYEEGYHAHQRNDDNASKTQDQRGRRGQQRQQQ
                     WSQWQPKPRKKRGALFWTLLIIILGLFVSLPFDFEDGFFPGLLLLAIIALAVTFLVRL
                     FSRSSGVSQTETHTFTVKAQPKIIVKGSSGTFRVHSSGEPDQVIVQATKHSSGLIGNP
                     DRAAVQYEQNNEKNRITIKSRSGWHVLGRNSVEIDITVPRMADLEIKTESGSIAVSDV
                     RGQMSLVSEAGSVKASGVMLRGDSRLKTDAGSITFSGSLHHSGSYSMTTDAGSVNVTL
                     PAEASFRLDARTDVGSINSPFPLAIQRDFPGAKARCEVGPGPYPSLKLRTDVGSINVW
                     QS"
BASE COUNT          657 a          732 c         1039 g          783 t
ORIGIN      
        1 gcgatgctgg ccatcgccct ggctactctc accctggatt cacttctgta gaatagaatg
       61 atatacagtg attaagtgag tgtcgaaagt aaagtcagcc aggatcagga aggttgtatg
      121 aatcaacggc aatgtttctg gctacgggat cgctttgcct caaagagcat tcagagccgc
      181 ttgagagggg gtgttcttct tctcctcttg ttacttactt cttgcgctgg cacaacaaat
      241 ccaccagtaa gtcaggtgac accttttgta actcgcatgc caatcttgca aacttcccag
      301 acggggggca ggggcgctat ccagcattat gagtatgtgt tcccggatgg ggagatggat
      361 gtgtatgata tggatcatgg gcaggcgctg gtgcagcggg tcgctttgcc gacgttcgct
      421 ggtgtgcgag gtgtggttgc cagcccggtc acgcatatgc tgtatgtgag ttatgggggc
      481 gatagcggcg atagcggcac gggcggctcg ctgctcaaat atgacttgct cacgcagcgc
      541 attgtctggc aaaggaccta tgcgcatggc attgatagta tggcaattac gcctgacggc
      601 aagaccattt atatgcctga tggtgaattg tcgagcgatg ggagatggta tattgttgac
      661 gcgggcagcg gcaatgagac cgggtttatc gatgcaggaa cagggccgca caatacgctg
      721 gtcagtctga atgggaagca tgtctatttg ggcggcagac atactccgta tctcaccgtt
      781 gttgacacgg cgacaaatca ggtcattcag gaaattggac ctctcaaggg tgatggagtc
      841 aggccgttta cgattaatgg gcgggaaacg ctcgcctata tgagtacgac aggcttctta
      901 gggtttcaag ttggggatat tcagacggga aaaattctct atacggtccc gatccaggga
      961 tttagttggg atgggagtgg agctactgat ccgagtcatg gtatttcact ttctccagat
     1021 gagaaggaac tctatgtgat cgactgggcc aatagttacg tgcatgtctt cgatgtctcc
     1081 catgttcccg cctcggctcc caggcaggtt gccgatatcc ggttgacacg gtcgatgcgt
     1141 cacaatgaaa gcccctgtgc atacgattgc ctggcagacg gctggctgca acatagcgcg
     1201 gatgggcggt tcgtctatgt gggggatgaa ggggatgtga tcgacacggc atcgcgtcag
     1261 gtggtagcga acctggatac cctttataac acacgcaaaa tgctggaaat cgattggcgg
     1321 ctaggggtgc ctgttttcac tacgtccagg caggggatgg gttatgtgac ttagcaggct
     1381 ggtagaattg tgtacaatag ctaaaaagtg aggtaacgct atgcctggca catcactggc
     1441 gaaaaaactg gtcatcaagc cacattatcg tattgtgctg ctgaatgcgc cgcgtgggta
     1501 tcgggagctg gtgggcgaat tgcccgatgg ggttacggtc gcgacggatg gcgatgggct
     1561 gtttgatatg gtgcaggcgt ttgcggaaca acgcttgcgg cgattgcgcc ggtggcgatc
     1621 caggcggtca agccggatgg actgttatgg ttcagctatc ccaagaagac cgggaagatc
     1681 gcgtcggacc tctcgcgaga ggtgacgtgg gaggtgattg agccgttggg ttggagagcg
     1741 gtgacgcaaa tttctgtcga tgaaacgtgg tcggcgctga ggttcagacc aggggagatg
     1801 gacagttagt ggtattttgt catgctgagc gcagcgaagc atgacatcag gtaggaggcg
     1861 actctggcgg tctcgctgga atcagctttg ccacacgttg atcgagccta catccgtgcg
     1921 cagtttgagc gaggggtagg ggccggggcc gacttcgcag cgggcttttg cgccggggaa
     1981 gtcgcgctgg atggcgaggg ggaaggggct gttgatactg cctacgtcgg ttctggcgtc
     2041 aaggcggaat gaggcttcag cgggcagagt gacattaacg ctgccggcgt cggtggtcat
     2101 gctgtaggag ccgctatggt gtagcgaacc ggagaaggtg atggaaccgg cgtcggtctt
     2161 gagccgggag tcgccgcgca gcatcacacc gctggctttg acggagccgg cttcgctgac
     2221 caggctcatc tggccgcgca cgtcgctgac cgcgatgctg ccggattcgg ttttgatctc
     2281 caggtcggcc atgcgcggga cggtgatgtc gatttcgacg ctgttcctgc ccaggacatg
     2341 ccagccggac ctggatttga tggtaatgcg gttcttctcg ttattctgct cgtattgcac
     2401 tgcggctcta tctggattgc ctattagacc agaagaatgc tttgtagcct gcacaatgac
     2461 ctggtccggt tcgccgctcg aatgcacgcg gaacgtgccg gagctgcctt tgacgatgat
     2521 ttttggctgc gctttcacgg tgaaggtgtg ggtttcggtt tgtgagacgc ctgacgatct
     2581 gctaaaaagt cggacgagaa atgtgacggc cagggcgatg attgcgagga gcaggagacc
     2641 ggggaagaag ccgtcttcga aatcgaaggg aaggctgaca aacaggccga ggatgatgat
     2701 gagcaacgtc cagaacagcg caccgcgttt cttgcgcggc ttcggctgcc actggctcca
     2761 ctgctgttga cgctgctgac ccctccgacc cctctggtct tgcgtcttcg atgcgttgtc
     2821 gtcattccgt tggtgtgcgt ggtagccttc ttcgtagggt gtgtagtcgg cgtcgtctcc
     2881 ggtgtcgatc ttttcgccag aagcgctctg gaaatctgct tttggtgtgt tactcaactg
     2941 agcatcattt atttgctgta tggggggtgc aggagatata ggcattgatg cgcgtggtgg
     3001 ggtagaggcg cgcagttggg tcggctgcca atcggggtcg gcgaaatgca tctcctggtc
     3061 gctcatacat acctcgcttc tgctccgtaa tatatcacat tatgtacgtc acatgggaat
     3121 gcgaggttgc aggttgtgtt ggaacaggac gcgatgatga caatcaaata ttgaatcggg
     3181 tatatgcatc tgtaggtgcc gatttatcgc g
//
LOCUS       DASZAK010000901         2136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_272352, whole genome shotgun sequence.
ACCESSION   DASZAK010000901 DASZAK010000000
VERSION     DASZAK010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272352"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1063)
                     /locus_tag="VGT44_15830"
     CDS             complement(80..1063)
                     /locus_tag="VGT44_15830"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV2582325.1"
                     /translation="MTAVTYLEAINQAIREEMQRDEAVFLLGEDVGNYGGAFKVSAGL
                     LDEFGSERVIDTPMAEAAIIGSAVGAALMGMRPIAEMQFMDFITCGFDQIVNMASKMY
                     WRSGIPAPLVIRGPAGGGTKGGPFHSLSPEAWFFHVPGVKVVVPSTAHDAKGLLKAAI
                     RDNNPVLYLEHKLLYRLPELREELPEEDYVVPIGKAITRRAGEHMTVLTYGAMVHTCL
                     KAAQTLSDEDHVEAEVVDLRSLVPLDREAILGSVRHTNKVLIVHEDTLTGGVGAELAA
                     ILAEDLFEYLDGPITRVAAPDAPFPYAPALEAAFLPNAEKVLAAARKLAAY"
     gene            complement(1060..>2136)
                     /locus_tag="VGT44_15835"
     CDS             complement(1060..>2136)
                     /locus_tag="VGT44_15835"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV2582326.1"
                     /translation="YLMRLTRAIEDRTRTLFLQGRIVGGVYTAQGHEATTVGAAILLG
                     EGDFIVPQHRDLGMHLTRGVSPRAVMCQWLARGNSPTLGRDGQLHIGDIQHGIAPSIS
                     MLGESLPVACGVALTMKMRKRQNIVMASCGDGATNTGPFHEALNFASVQKLPVVFIVE
                     NNGYAYSTPTTKQFAIENLSDRAKAYGMPGETVDGNDVLAVMETVGRAVEHVRAGKGP
                     ALVECKTFRVRGHSEADKADYVPQEVREAWLKKDPITRFEHYLVEQQMLTDAIKVEVE
                     RKIKAVVDDAVRFAEESPAPDGESVADYVYAPPGPIAIIGGPGADDPRFVNALDTRTS
                     QPYTTVADASAEVTRFAPEEVGKR"
BASE COUNT          391 a          680 c          649 g          416 t
ORIGIN      
        1 gattgtgcgc ctccctgggc gggcgaccat aaaggctcgc ccctacggat gattccaaca
       61 ttatttcgta aaactgcatt taataggccg ccagtttgcg cgcggcagca agcacctttt
      121 ccgcgttggg caggaaggca gcttccagcg cgggcgcgta ggggaacggc gcatcgggcg
      181 cggccacgcg cgtgatcggc ccatccaggt actcgaacag gtcttccgcc aggattgccg
      241 ccagctccgc gccgacaccg ccggtcagcg tgtcctcatg cacgatcaag actttgttgg
      301 tatgcctgac cgaccccagg atggcttcgc ggtctagcgg cacgaggctg cgcaggtcaa
      361 cgacctccgc ctcgacatgg tcttcgtctg agagggtttg cgctgccttg aggcaggtat
      421 gcaccatcgc gccataggtc agcacggtca tatgctcacc tgcccggcgc gtgatggcct
      481 tgcctatcgg cacaacatag tcttcttccg gcagctcttc gcgcagttcg ggcaggcgat
      541 agagcagctt gtgttccagg tacagcaccg gattgttgtc gcgaatggcc gctttgagca
      601 agcccttcgc gtcatgcgcc gtcgagggaa caaccacctt gacgcccggc acatggaaaa
      661 accacgcttc cgggctgagg gaatggaacg gcccgccttt ggtgccgccg cccgccgggc
      721 cgcgaatgac cagcggcgcg ggtatgccgc tgcgccagta cattttgctc gccatgttga
      781 cgatctggtc gaagccgcag gtgatgaaat ccatgaactg catctcagcg atgggacgca
      841 tccccatgag agccgcgcca acggcagagc caatgatggc cgcctccgcc ataggcgtat
      901 cgatgacgcg ctccgacccg aattcgtcca gcagaccggc gctgaccttg aacgcgccgc
      961 catagtttcc cacatcttcg ccgagcagga aaaccgcctc gtcgcgctgc atctcctcgc
     1021 gaatggcctg gttgatggcc tcaagatacg taacggctgt catcgcttgc ctacctcctc
     1081 cggcgcgaaa cgggtcactt ccgcggacgc atcggcgaca gtggtatagg gctggctcgt
     1141 gcgggtatcc agcgcgttga cgaaacgcgg atcgtccgcc cccggcccgc cgattatggc
     1201 gattgggcca ggcggcgcgt agacgtaatc ggcgaccgat tcgccatccg gcgcggggct
     1261 ttcttcagca aaacgcacgg catcatcgac gacggccttg atcttccgct ccacttcgac
     1321 cttaatagca tcggtcagca tctgctgctc aactaaatag tgttcaaagc gcgtaatagg
     1381 atctttcttg agccaggctt cgcgcacttc ctgcggcaca taatcggctt tgtccgcctc
     1441 ggaatgcccg cgcacgcgaa aggttttgca ctcgaccagc gcgggtcctt tgccagcgcg
     1501 cacatgctcg acggcacgcc ccacggtttc catgaccgcc aggacatcgt tgccatcgac
     1561 cgtttcgcct ggcatcccat acgctttggc gcggtcggaa aggttctcga tagcgaactg
     1621 tttcgtcgta ggggtggaat aggcatagcc gttgttttcg acaatgaaga cgacgggcaa
     1681 tttctgcacc gaggcgaagt taagcgcctc gtggaacggg ccggtattgg tcgcgccatc
     1741 cccgcaggag gccatcacga tattctggcg cttgcgcatc ttcatggtca gcgcgacccc
     1801 gcaggcgacg ggcagcgatt cgcccagcat actgatgctc ggcgcgatgc cgtgctggat
     1861 gtcgccgatg tgcagttgtc catcgcgtcc cagcgtgggg gagttgccgc gcgccagcca
     1921 ctggcacatc accgcgcgcg gagagacgcc gcgcgtgaga tgcatcccca ggtcgcgatg
     1981 ctggggaacg atgaaatcgc cctcgcccag caagattgcc gctccaacgg tggtggcctc
     2041 gtgtccctgc gccgtgtaga cgccgcctac tatgcgcccc tgcaagaaga gggtgcgggt
     2101 gcggtcttcg atggcacggg taagacgcat gaggta
//
LOCUS       DASZAK010000902         1595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_198598, whole genome shotgun sequence.
ACCESSION   DASZAK010000902 DASZAK010000000
VERSION     DASZAK010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198598"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /locus_tag="VGT44_15840"
     CDS             <1..1464
                     /locus_tag="VGT44_15840"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEV2582327.1"
                     /translation="IIGLDIGTTDCKAIALDERWQVMASFQEKYELRVPRPGWAEQDS
                     EEVWRAVAKALADLSSRLPTSEVLGIGLSGAMHSLIMLDEHEKPLAPAMTWADQRAAD
                     IAQALRQRTDPIKYYQRTGCPLQAIYHPAKARWCVDVSGQKFARLVSIKEFILYRLTG
                     IWAIDLGLASTTGLLDMHRRRWDAEALSLAGVAVSQLSPLVSSQAVVGSITPEAAHLT
                     GLRAGLPVVAGGSDGGMAMVGVQEHTVITVGTSGAVRRVDATPMLDPQARTWCYIQDD
                     AQDKIQRDTQGNANNGERAGRCWLIGGAINNGGLAAQWAKEKFYPHEEFETMFQEVSK
                     IEAGSAGVMCLPYLAGERTPLWDADLRATLSGFGLEHSRAHLARAVLEGVGYCLADVW
                     EIIDSGSGDTSVKLTGGITHSPVWSQIVCDILGVPLRIARGADASATGAALTAGEALG
                     KPISLPQEEPTTLAPDPSRHATYQKLHRAFQERRRKQ"
BASE COUNT          329 a          467 c          508 g          291 t
ORIGIN      
        1 atcatcggtt tagacatcgg cacaacagat tgtaaagcca tcgccctgga tgagcgatgg
       61 caggtcatgg cctcgttcca ggagaagtac gagctgcgcg tccccaggcc ggggtgggcc
      121 gagcaagaca gcgaggaggt ctggcgtgcg gtcgcgaagg cgctggcaga cctgtcatcc
      181 aggctaccta cctcggaggt actggggatt gggctgagcg gggcgatgca cagcctcatt
      241 atgctcgatg aacatgaaaa accgctcgcg cccgctatga cctgggcgga ccaacgcgcc
      301 gccgacatcg cccaggcgct gcgccaacgg accgacccca tcaagtacta ccagcgtacc
      361 ggctgtccgc tccaggcgat ctatcacccg gccaaggcgc gctggtgcgt tgatgtgtcc
      421 gggcagaaat ttgcgcggct tgtctccatc aaagaattta tcctctatcg tctgacgggc
      481 atctgggcta tcgacctcgg cctggcatcg acgaccggcc tgctcgacat gcatcgccgc
      541 cggtgggacg ccgaggcgct ttcgctcgcc ggagtcgccg tatcgcagct ctcgccactc
      601 gtctcatcgc aagcagtggt gggaagcatt acgccggagg cggcgcatct gaccggattg
      661 cgggccgggc tgccggttgt agccggaggc agcgatggcg gtatggcgat ggttggtgtg
      721 caggaacaca cggttattac cgtcggcacc agcggcgcgg ttcgccgtgt tgacgcaacg
      781 cccatgctcg atccccaggc gcgcacctgg tgttatatcc aggatgacgc acaggacaaa
      841 atacagagag atactcaggg aaatgcaaac aacggcgaac gagccgggcg gtgctggctg
      901 atcggcggcg cgatcaataa cggcggcctg gccgcgcaat gggcgaagga aaagttctac
      961 cctcacgagg aattcgagac gatgttccag gaggtatcaa agatcgaagc ggggtcggca
     1021 ggcgtgatgt gcctgcctta cctggccggt gagcgcacac ctctctggga tgcggacctg
     1081 cgcgccacct tgagcggttt cggattagaa cacagccggg cgcatctcgc gcgcgcggta
     1141 ctcgaaggcg tgggatactg cctcgccgat gtctgggaga ttatcgatag cggttcaggc
     1201 gacacaagcg tcaaattgac gggcggcatc acccattcgc cggtatggtc tcaaatcgtc
     1261 tgcgatatcc tcggcgttcc cctgcgcatt gccagaggag ccgatgcatc ggctaccggc
     1321 gcggcgctga ctgctggcga ggcgcttggt aagccgatta gcctgcctca agaggagcca
     1381 accacgctcg ctcctgaccc atccaggcat gcaacctacc agaagttgca ccgagcattc
     1441 caggaacgca gaaggaagca atagcctcga agacgaatta tagcaggcgg atgggatgat
     1501 tggattgatt cccctttcag gctctatgtg agtcagcatc gtcggcgcgc gaaaacggtt
     1561 cgctgtcttg cacatcctgt aagagactga gcaat
//
LOCUS       DASZAK010000903         9388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_628397, whole genome shotgun sequence.
ACCESSION   DASZAK010000903 DASZAK010000000
VERSION     DASZAK010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628397"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VGT44_15845"
     CDS             <1..426
                     /locus_tag="VGT44_15845"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEV2582328.1"
                     /translation="VHLMLELYGCDRQLLENESLVRRALDEYPARVEMEKVSPVHLYQ
                     IETSNPLDAGLSGFVVIAQSHISLHAWPEYGEVDIDICSCKEFSQEDAIAFAKDIFQT
                     EDIESHFVVRATRSLRPESPDPALLEEALAARRTRVGVR"
     gene            449..1324
                     /gene="speB"
                     /locus_tag="VGT44_15850"
     CDS             449..1324
                     /gene="speB"
                     /locus_tag="VGT44_15850"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEV2582329.1"
                     /translation="MNETLVTQFGAPDAPFCDPATARVTIIPAPLEYSVCYMKGTERG
                     PQAILDASSQMELYDEELDWSPVEIGFFTRPALDYTGMDHAAALAATGQAAREVLQRG
                     QLPLILGGEHSLSAPPIAAACEMFPDLTVLHIDAHGDLRDEYEGSPLSHACIERRVVN
                     MGIPLTEIGIRSFSPEEAAFMKTSPNVAIVWDYQLARGSAVLPWERLSQHTYLTIDLD
                     ALDPADMPAVGTPEPGGLRWHQLLDLLRDIFRRTTVVAMDVVELCPMPGQARADFLAA
                     KLVYKMIGYRFYDER"
     gene            1402..2385
                     /locus_tag="VGT44_15855"
     CDS             1402..2385
                     /locus_tag="VGT44_15855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582330.1"
                     /translation="MDSFSAARIRTAGKYTVLCFCLLLLLLAACDTAQNNPGQPNHTV
                     STNNGPTISYNADPQELVIRTFNGGGRLGTLEVSPEISIYGNGSYILGPGLTMQTGQL
                     STDTLDQLLRTLVGSDGLLNFSQQQFYDIPDQNATFLQLNIDQHSYNYLYGPFGNLAE
                     SAQAMVEYRQLGAALTSIRGMLTGALQGYKGSSTALLVHRDFSPDLTQAIPYWTEPDI
                     DLTNLAIYECGAIPQDQTGPNADTGCLTYTIPRHALLLSEQQQQAIVHLLQGQAQGVF
                     LERGVYYTVALRVLLPDELAARTLAMLGSDELSYSGIALIEGPVPTPQPTP"
     gene            2606..3838
                     /locus_tag="VGT44_15860"
     CDS             2606..3838
                     /locus_tag="VGT44_15860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582331.1"
                     /translation="MTRNFKQHGRDDERPYSRQSSDRYEGEQSSRPARPRPNRASVDR
                     AWENGARQQHADYHPRNPHNTQNARNAHNPMPRQPGQPYQPNRRYNTSENASSTPDSR
                     NYRQPPFQRQENRRFDDRPPRRYEGPRPYDSGHRTSEGGYNRERPRYSREGEQQDRRP
                     SSYRDQERRSSTYRDQEHRSSYRDEQRAPRPYTPRPYAPREDARGYGGAPRRSSRADY
                     PNRAGGQEKRFDRRENRPETRNPRFQSRPAQFSPQPRPFRPNANDDELFEGDYERFDA
                     RGHTAQRQERHVTELPDGRVLKGNRKEQREAAAFWTDVNEEVQELIPNEVKDTSTEQP
                     AEPTPAPTKRAATGKKVTSKTQTTRTPKATEAKSKRAASENGEPRARKTSAKAKGVKP
                     AKSGTRPSQRGYKWPQSE"
     gene            3838..4782
                     /locus_tag="VGT44_15865"
     CDS             3838..4782
                     /locus_tag="VGT44_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2582332.1"
                     /translation="MQYPSYLQLYETGELAERAAAAWEVLRSCTICPQNCRCNRIAGK
                     TGVCHSGTEAIVASWNVHRREEPPISGSRGAGTIFFGYCQARCTYCQNFSLSQGGLGH
                     RVGPERLADMMMYLQKKRCHNLDLVTPTHFVPQILAALVVACGKGLRLPLVYNCAGYE
                     NLETLQLLDGVVDIYLPDSKYSDNKHALRTSKMPHYVDSNQAALREMHRQVGPLVLDD
                     EGIARRGLLIRHLVMPEDISGTREVLRWIADELGPDTPVSLMDQYFPAWKAVNDPILN
                     RRLTWSEYRTALDALEEFNFTAGYIQEDLMELDQTSCV"
     gene            4876..5865
                     /locus_tag="VGT44_15870"
     CDS             4876..5865
                     /locus_tag="VGT44_15870"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV2582333.1"
                     /translation="MNDTATLSRLFAGIQSDLEQVDSTFEVRATSGLDILNSASMHAL
                     ASPGKRLRTALTLLSGKLSTYRFDKLLPLSVAFEMVHLATLIHDDIVDNARTRRGNPT
                     VNAIWGDNIAILLGDYYFAKTAGLIADINDNRIDHLFSDTVATVCEGTIMEMMTARRI
                     DLTIENYYEKIRHKTACLIAACCKGGAIVSQASDEEIALLYEYGINLGIAFQIIDDIL
                     DYTEDQATIGKPAGNDLRQGMVTLPLIYALQQQPLNGHIQHIHSVLNGADQDEEEIRS
                     VVAWVVDGDGVSQAQTDANRYAENARASLYQFPPSSNRDTLDELIDFVVIRRR"
     gene            complement(5871..7139)
                     /locus_tag="VGT44_15875"
     CDS             complement(5871..7139)
                     /locus_tag="VGT44_15875"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582334.1"
                     /translation="MRTAPQHPLRSTDRLDQDVTMQSAYSTQVAQQRAMVSQPLVARP
                     LTPGTRLRGNRYRLLESLERQDWLSGTYEAIWLGQDAHRSGAQVVICEVTLPEPNSIM
                     TQTLLRTATMSLVNVGRHRHIPALWDAFSDQGRGFFVFEPVDGESIVARLRYSGRPMP
                     EQEVIECCLQMTEVLELLAQQSPPFAHGLIRPEHILVGRNGSHYYLTTFSVVLAGGGT
                     QFLTGIERSRLSPYVAPEFARGVADSRTDVYSLIATAYYAVTGTAPTGISGSIPPAQR
                     INNAISPAFDAILTRGLRSIAGQRYQRPSELRQDLLALRSVNGTIEPGGQRDRRSEQA
                     YQWDAPTSAAANRQPVGRTYNGQSVPYADALPIRLAIDVPEEEQVILLPKPEEMPSMP
                     PANERLNAALLLGAILVALIILVVLTQVPL"
     gene            7193..7525
                     /locus_tag="VGT44_15880"
     CDS             7193..7525
                     /locus_tag="VGT44_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582335.1"
                     /translation="MVVSSWSLLDYPAALYAAHAFQMLAGESHREKPYVGPHPWQRFV
                     EESIALAFRQHPASRCLPEMIGSTGAVDKATMAAGTTCFHQAVDPTPSQQETSLTALP
                     GEACLTAG"
     gene            complement(7869..8270)
                     /locus_tag="VGT44_15885"
     CDS             complement(7869..8270)
                     /locus_tag="VGT44_15885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582336.1"
                     /translation="MPICPICDDTGLQNSDSTNPEFCYCAAGKQARRQWEASRGGGRG
                     KNQPKPASFIDIANLDDVNASVDAMRASLLGSLAHEATPTRHPAVQLLFTVAHANHEV
                     SVSEATQLYELAKALEAFLDGLSPVGRNVKR"
     gene            complement(8336..>9388)
                     /locus_tag="VGT44_15890"
     CDS             complement(8336..>9388)
                     /locus_tag="VGT44_15890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582337.1"
                     /translation="GQFLGAWYPTSDIALLSCVVFLLIRSQGRIYQARARRVSLLVVG
                     IGLCVFATSDFIFNIQQNAGTYVDGTWIDLGWPFGMMIIGVAAHLRRFLPGTSHEAIE
                     QRVRRQRERVGFSLAQVVPYALLASLFIVLAINVLAKNSNQDSIRPVLVYTTLVVVAL
                     LVLRQILTQLDNERLTRRQATALDRLEVANARVEEQARMITERNAALEEGIAHLKDVQ
                     AQLLNGDLGARARLVGGDLLPLAGSLNLMADRLSRFEQADQHSQRLYSALADLSVTLE
                     RYRVGTPFLVPPSCQDIPEIQRLLLALGLRQAMPTSVGRFSSQPHTSRALPLMADTPP
                     SVPRTAPGVPLRRREQ"
BASE COUNT         2068 a         2819 c         2680 g         1821 t
ORIGIN      
        1 gtacatctca tgcttgaact ctacggttgc gaccgtcaac tgttagagaa cgaatcgctg
       61 gtgcgacggg cgctggacga atatccggcg cgtgttgaaa tggagaaggt cagccctgtc
      121 catctctatc aaatagagac atccaatccg cttgatgccg ggctttcggg cttcgtggtg
      181 atcgcacaga gccacatcag cctgcatgcc tggccggaat atggcgaggt cgatatcgac
      241 atctgttctt gcaaagagtt cagccaggag gacgctatcg ccttcgcgaa agacatcttc
      301 cagaccgaag atatcgagtc ccattttgtt gtgcgcgcca cccgttcgct gcgcccggag
      361 tcgcccgatc cagctctgct ggaagaggcg ctggcagccc gcagaacgag agtaggggtg
      421 cgctaaagac aaggagcatt ttaccccgat gaacgagacg cttgtgaccc agttcggcgc
      481 gcccgatgcg cccttttgtg atcccgccac tgcccgcgtc accatcatac cggccccgct
      541 ggaatatagc gtgtgctata tgaaaggcac cgaacgcggg ccacaggcga ttctcgatgc
      601 gtccagccag atggaactct acgacgagga actggactgg agtcctgttg aaatcggctt
      661 ctttactcgt cccgcgcttg attacaccgg catggatcat gccgccgcgc ttgccgccac
      721 cggacaggca gcgcgcgagg tgttacagcg cggccagctt cccctcatac ttggcggcga
      781 gcattcgctg tctgctccac caatcgctgc cgcatgcgaa atgttccccg acctgaccgt
      841 gctgcatatc gacgcgcacg gcgatctgcg cgacgagtac gaaggcagcc cgctttctca
      901 tgcctgcatc gaacggcgcg tggtcaacat gggcattccc ctgacggaga tcggcattcg
      961 cagcttcagc ccggaagagg cggcgttcat gaagaccagc cccaacgtgg cgattgtctg
     1021 ggattaccag cttgccaggg gcagcgccgt tcttccctgg gaacgcctca gccagcatac
     1081 ctacctgacg attgacctcg atgcgctcga ccccgccgac atgcccgccg tcggcacccc
     1141 ggaaccgggc ggtttgcgct ggcatcagct actcgacctg ctgcgcgaca tcttccgacg
     1201 cacaacagtg gtcgcgatgg acgtggtcga actctgcccc atgcctggcc aggcgcgcgc
     1261 cgactttctg gccgccaaac tcgtgtataa aatgattggc taccgctttt atgatgagcg
     1321 gtaagatgta gtatcctgct tgttcgccgc ctcgtttaca atagaaggta gagacattta
     1381 tcgaggcaca ggagcgaaac attggattcc ttttcagcag caagaatacg tacagcgggc
     1441 aaatacaccg ttctttgctt ctgcctgttg ctgctattgc ttgccgcctg cgataccgcg
     1501 cagaacaacc ctgggcagcc gaatcatacc gtctctacca ataacgggcc gacgatttct
     1561 tataacgccg acccgcagga acttgtcatt cgcacgttta atggaggtgg taggctggga
     1621 actctggaag tcagcccgga gatcagcatt tacgggaatg gcagctacat cctggggcca
     1681 ggattgacga tgcagacagg gcaactctcg acagacacgc tcgatcagct cttgcgcacg
     1741 ctggtcggta gcgatgggtt gttgaacttc agccagcagc agttttacga catccccgat
     1801 cagaatgcga cgtttttaca gttgaatatt gaccagcaca gctacaatta cctgtatggc
     1861 ccgtttggga atctcgcgga gagtgcgcag gctatggtcg agtatcgcca gttaggtgcg
     1921 gccctgacca gcattcgcgg gatgttgaca ggcgcgctcc agggatacaa aggcagcagc
     1981 acggccctgc tggtccaccg ggacttcagt ccggatttga cccaggccat tccttactgg
     2041 actgagccgg atattgacct gaccaatctc gccatttacg aatgcggcgc gattccacaa
     2101 gatcagaccg gccccaatgc tgataccggc tgcctgacct ataccattcc acgtcacgcg
     2161 ctgttgctga gcgagcagca gcaacaggcc atcgtacacc tgctgcaagg acaggcacaa
     2221 ggagtctttc ttgaacgagg agtctactac accgtcgcgc tgcgagtgct gctgcccgat
     2281 gaactcgccg ccaggacgct tgccatgctc ggcagcgatg aattgagtta tagcggcatc
     2341 gcgctgatcg agggaccggt gccaacgccg caaccgacgc cgtaggacgg caatcggtag
     2401 cgcgaaagtg ccaggctatc gccacaacgg gcgtagtgtg gtatactacg tgcaggcgct
     2461 accttcatgt atgttcagat caacagatcg cgcttatccc tggagcgagg gcggaatgac
     2521 cgacgggttt ttcaattgtt aggaaacgtg ccagaccgtt accccgtaag ctaacaacct
     2581 ccactgtacc atttggaaag gacacatgac cagaaacttc aagcagcatg ggcgagacga
     2641 cgagcgaccg tattctcgcc aatcatccga ccgctacgaa ggggagcaat cttcaagacc
     2701 ggctcgccct cgtcccaacc gggcgtcggt cgatcgcgcg tgggaaaacg gcgcacgcca
     2761 gcagcacgcc gattatcacc cacgtaaccc acataacact cagaatgcgc gcaacgcaca
     2821 caatccaatg ccccgtcaac caggccagcc ctatcaaccg aaccggcggt acaacacctc
     2881 tgaaaacgca tcctctaccc ccgatagtcg caactaccgc cagccgccat ttcaacgtca
     2941 ggaaaatcgc cggtttgatg atcgcccgcc gcgccgttat gagggtccgc gaccttacga
     3001 ctcaggccat cgcacctcag agggtgggta caatcgtgag cgaccgcgtt attctcgcga
     3061 gggcgaacaa caggatcgcc gcccctcgtc gtatcgcgac caggaacgtc gctcttccac
     3121 gtatcgcgat caggaacatc gttcctcata tcgcgacgag cagcgcgcac ctcgccccta
     3181 tactccacgc ccttatgccc cgcgtgaaga tgcgcgtgga tacgggggcg cgccgcgacg
     3241 ttcgagccgc gccgactacc ccaaccgtgc cgggggacag gaaaagcgat ttgatcgcag
     3301 ggagaaccgg cctgagacgc gcaacccacg cttccagagc aggccagcgc aattctcgcc
     3361 ccagccgcgt ccattcaggc caaatgcgaa cgacgacgaa ctcttcgagg gtgactacga
     3421 acgttttgac gcgcgcggtc ataccgccca acgccaggag cggcatgtga ccgaactgcc
     3481 cgatgggagg gttctgaaag gaaatcgcaa agagcagcgc gaagcagcgg cattctggac
     3541 agacgtgaac gaggaggttc aggagctgat accgaacgag gtgaaggata cctccaccga
     3601 gcagccagca gaacccactc ctgctcccac gaaacgagcc gcgaccggca agaaggtcac
     3661 gagcaagacg cagacaacca gaacgccaaa agcgaccgaa gcgaaaagca aacgcgccgc
     3721 aagcgagaac ggcgaacctc gcgccaggaa aacgtccgcg aaagccaaag gtgtcaagcc
     3781 tgcaaaaagc ggtactcggc cctcgcagcg cggctataag tggccacaat ctgaataatg
     3841 cagtatccga gctacctaca attgtacgaa acgggcgaat tggcggagcg cgcagccgcc
     3901 gcatgggaag tgctgcgcag ctgcaccatt tgcccgcaga actgccggtg taatcgcatc
     3961 gcgggcaaga cgggcgtctg tcactcaggc acggaggcta tcgtcgcgtc gtggaacgtt
     4021 caccggcgcg aagagccgcc catcagcggc tcccgtggcg cgggtacgat cttctttggg
     4081 tattgccagg cgcgctgcac ctattgccag aacttctcac tcagccaggg cggactcggc
     4141 catcgcgtcg ggcctgaacg actggctgat atgatgatgt acctgcaaaa gaaacgctgc
     4201 cataacctcg acctggtgac acctacccat ttcgtgcctc agatactggc cgcgctggtg
     4261 gttgcctgcg gcaaggggct gcgcctgccc cttgtctaca attgtgcggg ctacgaaaac
     4321 ctggaaacgc tgcaactgct ggacggcgtc gttgatatct acctgcccga ctccaaatac
     4381 tcggacaaca aacacgcctt gcgtacctcc aaaatgcccc attacgtcga ttcgaaccag
     4441 gcggcactgc gcgagatgca ccgccaggtg ggtccgcttg tcctcgatga cgagggcatc
     4501 gcgcggcgcg ggctgctgat tcgccacctg gtcatgccag aagacatctc cggcacgcgc
     4561 gaagtcctgc gctggattgc cgatgaactt ggccccgata ctcccgtcag cctgatggac
     4621 cagtatttcc ccgcctggaa agcagtcaat gaccccatcc tgaaccggcg attgacctgg
     4681 tcggagtacc gcaccgccct ggatgcgctg gaagaattca atttcacagc agggtacatc
     4741 caggaagacc tgatggaact ggatcagaca tcgtgtgtgt agccatgctt gatgtcgcac
     4801 acctgagaca tgggcgtggt tataccgtgc tataatctga aaaaacgata tttctgccag
     4861 gaacggggag gtcccatgaa cgacacggcc actttgagcc gactttttgc cggtatacag
     4921 tctgatttag agcaagtaga tagcaccttt gaagttcgag ccacgtccgg cctggacatc
     4981 ctgaactctg cctcgatgca cgcgcttgcc tcgcccggca aacgtctgcg cacggccctc
     5041 accttgctct caggaaaact cagcacctac cgcttcgaca aactgctgcc gctcagcgtg
     5101 gcttttgaga tggtgcattt ggcaaccttg atccatgacg acattgttga taatgcgcgc
     5161 acaagacgcg gtaatccaac ggtcaacgcc atctggggcg acaacatcgc gatcttgctg
     5221 ggcgactact acttcgccaa aacggcgggg ctgatcgccg acatcaacga caaccgcatc
     5281 gaccatctct tctccgatac ggttgcgacg gtctgcgagg gtaccatcat ggaaatgatg
     5341 accgccaggc gcatcgatct gaccattgag aactattacg agaaaatcag gcataaaacc
     5401 gcctgtctca tcgctgcttg ttgcaaaggg ggagcgatcg tgagccaggc atccgatgaa
     5461 gagatcgcgc ttttgtacga gtatggcatc aatctgggga tcgccttcca gattatcgat
     5521 gatattctgg attatacgga agatcaggct acgattggca aaccggcggg caacgacctg
     5581 cgccagggca tggtcaccct ccccttaatt tatgctttac aacagcagcc cttaaatgga
     5641 catatccagc atattcacag cgtcttgaat ggcgcagacc aggacgaaga ggagattcgc
     5701 tcggtggtcg cgtgggtagt cgatggcgat ggcgtgtcac aggcccagac cgacgcaaac
     5761 cggtacgcgg agaacgcgcg cgcgtcgctc taccaattcc caccttcgag caaccgcgat
     5821 acgctcgacg agttgatcga ttttgtcgtg atacgcaggc gctaacaggg ctataacggg
     5881 acctgggtca acaccaccag gatgatgagg gcaaccagga tcgctcccag gagcaacgcc
     5941 gcgttcaatc gttcgttagc gggcggcatc gaaggcatct cttccggctt cggcagcagg
     6001 atgacctgtt cctcttccgg tacgtcaatc gccagcctga tcggtaacgc atctgcatat
     6061 gggactgatt gcccattgta agtcctacct accggttggc gattcgcggc tgcgctggtc
     6121 ggcgcgtccc actggtacgc ttgctcgctg cgtctatccc gctgaccgcc gggttcgatg
     6181 gtgccattga ccgagcgcag cgccagtaaa tcctggcgaa gctccgacgg gcgctggtaa
     6241 cgctgcccgg caatggaacg caagcccctg gtgagaattg catcgaatgc cggggaaatg
     6301 gcattgttga tacgctgcgc cgggggaatg ctgccgctga tacccgttgg cgcggtcccg
     6361 gtgacggcat aataggctgt ggcgatcaaa gagtaaacat ccgtacgaga atcagcaacg
     6421 ccacgtgcga attccggcgc gacgtagggc gagaggcgcg atcgctcgat gcccgtgagg
     6481 aactgggttc ccccgccagc cagcacgacc gagaaggtcg tcaggtaata atgtgaacca
     6541 ttgcgcccta ccaggatatg ctccgggcga atcaggccat gcgcgaacgg gggggattgc
     6601 tgcgccaata gttccagtac ctcggtcatt tgcaggcaac actcgatgac ctcttgctcc
     6661 ggcatggggc gaccgctgta gcgcagacgg gcgacgatcg actcaccatc aaccggctca
     6721 aatacgaaga aaccgcgccc ctggtcgctg aaggcatccc acagggcggg aatatgccgg
     6781 tgcctgccca cgttgaccaa cgacatcgtg gcggttcgga gcagggtctg cgtcatgatc
     6841 gagttcggtt ccggcagcgt gacctcgcag attacgacct gcgcgccact gcgatgcgcg
     6901 tcctgaccaa gccagatcgc ttcatatgta cctgatagcc agtcttgccg ttccagcgat
     6961 tcgagcaagc ggtagcggtt tccgcgtagg cgtgttcccg gcgtgagcgg acgcgcgacc
     7021 aacggctggc tcaccatcgc cctctgttgc gcgacctgcg ttgagtaggc ggattgcatc
     7081 gttacatcct ggtcgagacg atctgtagag cgcagaggat gctgtggggc ggtacgcatg
     7141 gttgtacctt gataggacag accatcagtg ctcggcggag gtgtggtggg aaatggttgt
     7201 ttcgagttgg tctcttctcg actacccggc tgcactttat gcagcacatg cttttcaaat
     7261 gctggccgga gagtcgcatc gggagaagcc atacgttggc ccgcacccat ggcagaggtt
     7321 cgtggaggaa tcgatcgcgt tggcattccg gcaacatccg gcatcgcggt gtttgccgga
     7381 gatgattggt tcgactggcg ctgttgataa ggcgacgatg gcggctggaa cgacatgttt
     7441 ccaccaggct gttgacccga cgccctcaca gcaggaaact tccctgacgg ccttaccagg
     7501 ggaagcttgc ctgactgctg gctgatccct ggcatactgc cctgacgccg gcgtattgcc
     7561 ggaaagctgc cagaattttg cattgagttg gcaggaaatt cttgttcatt cgccgggcca
     7621 cccccatacg tcgcacccgg agcggcggtc atacggaccg ataacccaca atttgtacag
     7681 gaacccctgg tggtgtccca ctgaccacca caacgtggac ataacataac ggacccctcc
     7741 ctctctgtgt cgcctatgcg ctcatgctgt tactttatac acttcaagca tgttctcgct
     7801 ccttaatggt agaggagaac atgcttgtct gtcaatagat gttacccaat attactatat
     7861 cgtgcgcatt agcgcttaac attacgccct actggactca atccatccag aaacgcttcc
     7921 agcgctttcg ccagttcata caactgggtt gcctcgctga ccgagacctc gtggttggca
     7981 tgggcgacag tgaagagtaa ctgcacggca ggatggcgcg tgggggtcgc ttcatgcgcc
     8041 agcgaaccaa gcagtgatgc gcgcatggca tcgacactgg cattgacatc atccaggttg
     8101 gcaatatcga tgaaactggc aggcttgggt tgattcttgc cgcggccacc cccacgactg
     8161 gcttcccact gtcgccgcgc ctgcttgccc gccgcgcagt agcaaaattc tggattggtt
     8221 gagtcggagt tttgtagccc cgtatcatca caaataggac agatcggcat cagcagcctc
     8281 ctcatgtata ctatcagagt caaatgctgc tcgcactata ccatataatg acagattact
     8341 gctccctccg gcgcagggga acgcccggcg cggttcgcgg tacagaaggg ggggtatccg
     8401 ccataagtgg caacgcgcgc gacgtgtgtg gctgcgacga gaagcggccg actgacgtgg
     8461 gcatcgcctg gcgcagaccc agcgccagga gcagacgctg gatttccggg atatcctggc
     8521 aggagggcgg gactaagaac ggtgttccga cgcgatagcg ttccagggtc acgctcaaat
     8581 cggccagcgc actgtacaaa cgctgggaat gctgatccgc ctgttcaaaa cggctgaggc
     8641 ggtcggccat caggttcagg ctgcccgcca gcggcaacag gtcgccgccg accaggcgcg
     8701 cccgcgcgcc caggtcgccg ttgagcagtt gcgcctgcac atccttcaga tgcgcaatgc
     8761 cttcctccag cgccgcgtta cgctcggtaa tcatgcgcgc ctgttcctcg acgcgcgcgt
     8821 tggctacctc gagccggtca agcgcggtag cctgccgccg agtcaaacgt tcgttatcga
     8881 gctgggttaa aatctggcgg agcaccagca gggccacgac gacgagcgtc gtatagacca
     8941 gcaccgggcg tatgctgtcc tggttggagt tcttagcaag gacattgatg gccagcacga
     9001 taaacagcga cgccagcagc gcgtagggaa cgacctgcgc caggctaaag ccaactcgct
     9061 cgcgctggcg gcgcacacgc tgttcaattg cttcatgtga tgttcccggc aggaaacggc
     9121 gtagatgggc agccacgccg ataatcatca tgccaaaggg ccagcccagg tcgatccacg
     9181 tgccatcgac ataggtcccc gcgttctgct gaatgttgaa aataaagtct gacgttgcga
     9241 agacacatag cccgatgcca acgaccagca agcttacgcg ccgtgccctc gcctggtaga
     9301 tacgtccctg gctgcggatc aacaggaaga ccacacaact gagcagggcg atatcagagg
     9361 tcggatacca ggcccccagg aactgacc
//
LOCUS       DASZAK010000904         1611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_272413, whole genome shotgun sequence.
ACCESSION   DASZAK010000904 DASZAK010000000
VERSION     DASZAK010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272413"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VGT44_15895"
     CDS             complement(<1..938)
                     /locus_tag="VGT44_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HEV2582338.1"
                     /translation="MSDLLAARAQMGTSLAFHIIFASLGVGLPLLLCIAEGLALWHKD
                     SGWMTLTRRWTKAFALLFAIGAVSGTILSFEIGLLWPTFTRFSGAIIGLPFALEGFAF
                     FIEAIFLGLYLYGWERLSPRAHWLCSFPLWISGAASAWFIVSANSWMNTPVGFQITHG
                     QVTGINPLQAILNPSTPYETTHMLLAAYVAAGFGVAAVYAVQILRGRREAYFRKGLLL
                     AMALGAIAIPFQIFDGDLSARSLATLQPAKLAAMEGVFHTEQGAPIKIGGIVDDKTGQ
                     VLFALEIPDLLSILAKGDPHASITGLDAYAPQDRPDP"
     gene            complement(966..>1611)
                     /locus_tag="VGT44_15900"
     CDS             complement(966..>1611)
                     /locus_tag="VGT44_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2582339.1"
                     /translation="RRYTSAGVPITDLIQEGNLGLMRAAEKFDYRRGVHFGTYATWWI
                     RQAVSRAAADQSCLIHLPEHVAMRLRKVRRNAARLSQENGSDPLPEQIAEASAINLDE
                     VKKLLSLVEQPVSLDTPVDDETQYSLADSLEDTATSAPAETASQHLLGEELHQALDVL
                     TPRERAVITRRYGVGDGQSRTLLEIGKELGISRERVRQIEAAALKKMRCFYGCQ"
BASE COUNT          373 a          461 c          464 g          313 t
ORIGIN      
        1 ggatcaggtc gatcttgtgg cgcgtaggca tccagaccgg taatgctggc atgcggatcg
       61 cctttggcca ggatgctcaa caaatcaggg atttcaagcg caaacagcac ctgccccgtc
      121 ttatcatcaa cgatgccacc aatcttgatg ggggcacctt gctctgtatg gaagaccccc
      181 tccatcgctg ctaactttgc aggttggagc gttgcaagcg agcgggcact caggtcacca
      241 tcgaaaatct ggaaggggat tgcaattgcg cccagcgcca tcgccagcag caggcccttg
      301 cggaagtacg cctcgcgtct gccgcgcagg atctggacgg catagacggc agcaactccg
      361 aagccagctg ccacgtaggc tgccaggagc atatgggtcg tctcatacgg cgtgctcgga
      421 tttaagatag cctgtaaggg attgatgcct gtcacctgcc catgcgtaat ctggaagcct
      481 accggggtat tcatccagga gttcgcgctg acgataaacc acgccgaagc ggctccactg
      541 atccagaggg gaaaagaaca gagccaatgc gcacgcggcg aaaggcgctc ccacccatac
      601 aggtagagac cgaggaagat cgcctcgatg aagaaggcga acccctccag ggcaaacggt
      661 aagccgatga tagcgcctga gaacctggta aaggtcggcc agagcaggcc gatttcaaac
      721 gagaggatcg tgccggagac cgcgccgatg gcaaagagca gggcaaaggc tttcgtccag
      781 cggcgggtga gggtcatcca gccgctatct ttgtgccaga gggccagtcc ctcggcaata
      841 cacagcagca gtggcagccc cactcccagg gaggcaaaga tgatatgaaa agcaagcgac
      901 gttcccattt gagcacgcgc cgctaagaga tcagacatga agaatcctcc ttgatgtatc
      961 caagattatt gacatccata aaagcagcgc atctttttca gagctgctgc ctcaatctgt
     1021 cgcacacgtt cgcgtgagat acccagttct ttcccaattt cgagcaaggt acggctctgg
     1081 ccatctccaa ccccgtagcg ccgcgtgatg actgcacgtt cacgtggcgt gagcacgtcc
     1141 agcgcctggt gcagttcttc accgagcaga tgttgggagg cagtttctgc gggagcagaa
     1201 gtcgctgtat cttcaagcga atcggccagc gaatactggg tttcatcgtc tacaggcgta
     1261 tcaagagaaa ccggctgctc gaccaggctg agcagctttt tgacttcatc caggttgata
     1321 gcggaagcct cagcgatttg ctctggcagt ggatcggacc cattttcttg cgagagtcgt
     1381 gccgcgttac gtcgtacctt gcgtaaacgc atcgccacat gctcggggag atgaatcaag
     1441 caggactgat cggcagcggc acgactcact gcctggcgga tccaccaggt ggcataggtc
     1501 ccaaaatgca cgcctcgccg gtagtcaaat ttctcggcag cgcgcatcag ccccaggttt
     1561 ccctcctgga tcaggtcggt gatcggtaca cccgcgctcg tgtagcgacg c
//
LOCUS       DASZAK010000905         1885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_342521, whole genome shotgun sequence.
ACCESSION   DASZAK010000905 DASZAK010000000
VERSION     DASZAK010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342521"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1885)
                     /locus_tag="VGT44_15905"
     CDS             complement(<1..>1885)
                     /locus_tag="VGT44_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2582340.1"
                     /translation="RALKAAGLTRALVLKALGDIRGSQRVTDPHPEGKYQALEKYGRN
                     LTELAAQGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGLAQRIVR
                     GDVPKTLVGKQVVTLDLGALVAGAKFRGEFEERLKAVLKEITSGEGNIILFIDELHTL
                     VGAGAAEGAMDASNMLKPMLARGELHCVGATTLDEYRKHIEKDAALERRFQPIIIDQP
                     SVEDTISILRGLKQRYEVHHGVRIQDSALVAAAVLSNRYITDRFLPDKAIDLVDEAAS
                     RRRVELDSKPSEIDALERRILQLEVEQEALKKETDAASRERRSRVEQEMANLKEQLHS
                     LKLSLESEREPVEEIRRLKRELEQAQVDLEKAEREYNYEAMARLQYGTIKDLEQRIQT
                     LESKLTSRKGARMLKEEVDAEDIAEIVSKWTHIPVSKLMQGEVQKLLTMEDRLRERVV
                     GQDEALRIVSDAVRRSRAGLQDPNRPLASFMFLGPTGVGKTELARALAEFLFDDERAI
                     VRIDMSEYMEKHSVSRLIGAPPGYVGYEEGGQLTEVVRRRPYSVVLFDEIEKAAPEVF
                     NVLLQLLDDGRLTDGQGRTVDFKNTVVIMTSNVGGRWLHEYDGMDEDEIQRTVRQRLR
                     EEG"
BASE COUNT          345 a          582 c          510 g          448 t
ORIGIN      
        1 aaaccctcct cacgtagtcg ttggcgtacg gtgcgctgta tctcgtcttc atccatgcca
       61 tcatactcgt gcagccaccg gccacctaca ttacttgtca ttatcacgac ggtatttttg
      121 aaatcaacgg tgcgcccctg cccgtctgtc aaacgaccgt cgtcgagcag ctgcaagagg
      181 acgttgaata cctccggagc ggctttttca atttcatcga agaggacgac gctgtaggga
      241 cggcgccgca ctacctcggt aagctgacct ccctcttcgt agccaacgta gcccggaggc
      301 gcaccgatca gtcgcgaaac gctgtgcttc tccatgtatt cggacatgtc aatgcgcacg
      361 atggcccgct cgtcgtcgaa caagaactct gccagggcgc gtgccagttc tgtctttcca
      421 acgccagtcg gtcccaggaa catgaagctt gctaatgggc gattggggtc ttgcaggcca
      481 gcgcgagaac gacggacggc gtcagagacg atcctcaagg cttcgtcttg tcccacgacg
      541 cgttcgcgca ggcggtcctc cattgtgagc agcttctgta cctcaccctg catcaacttc
      601 gagacgggga tatgcgtcca cttcgagacg atctcggcaa tgtcctcagc atccacctct
      661 tctttgagca tacgcgcacc cttgcggctg gtcagcttcg attccagagt ctggatgcgc
      721 tgctccaggt ccttgatggt gccatactgc aaacgcgcca tcgcttcgta gttatattcg
      781 cgttcggcct tttccaggtc aacctgcgcc tgctctagct ctcgcttgag gcggcgaatc
      841 tcttccaccg gctcgcgttc gctctcgagc gacagcttca gcgagtgcag ttgctccttc
      901 agattggcca tctcttgctc aactcggctg cgtcgctcgc ggctggcagc atcggtctcc
      961 tttttcagtg cctcctgttc gacttccaac tgcaggatgc gacgttccaa cgcatcgatc
     1021 tcacttggct tgctatccaa ttccacacgc cgacgactgg cggcttcgtc aaccaggtcg
     1081 atggccttgt caggcagaaa acggtcggtg atgtagcggt tggagagcac ggcggcggcg
     1141 accagggcgc tatcctgaat acgcacgcca tgatgtactt cgtaccgctg cttcagtccg
     1201 cgcaggatgc tgatagtgtc ttcgaccgag ggttgatcga tgatgatcgg ttgaaaacgg
     1261 cgttccaggg cggcatcttt ctcaatgtgc ttgcggtact catccagtgt ggttgcgccg
     1321 acgcagtgca gttcgccacg tgccagcatt ggcttcagca tattggacgc gtccattgcg
     1381 ccttctgcgg caccagcacc caccagtgtg tgcaattcgt cgatgaaaag gatgatgtta
     1441 ccttcgccac tggtgatctc tttcaaaaca gccttcaggc gttcctcgaa ctcaccacga
     1501 aactttgctc cggcaactaa cgcgcccagg tcaagggtga cgacctgctt accaaccagc
     1561 gttttgggta cgtcaccacg cacgatgcgt tgtgccagtc cttcaacaat agccgtcttg
     1621 cccacgcctg gttcgccgat gagcaccggg ttgttcttcg tacggcggct cagcacctgg
     1681 atgacgcggc gaatttcttc gtcacgtcca atgaccggat cgagtttgcc ctgcgccgcc
     1741 agctcggtga ggtttcgccc atatttctcc agggcctggt atttaccttc aggatgcgga
     1801 tcggtgacac gctgcgagcc gcgaatgtcg ccgagggctt tcaggacgag ggcacgggtc
     1861 aggcctgctg ctttcagcgc gcgct
//
LOCUS       DASZAK010000906         3024 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_57774, whole genome shotgun sequence.
ACCESSION   DASZAK010000906 DASZAK010000000
VERSION     DASZAK010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57774"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="VGT44_15910"
     CDS             <1..1057
                     /locus_tag="VGT44_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2582341.1"
                     /translation="AGKTTTIRMLLGLIRPTQGGGRILGHDIENERAAILPHVGALVE
                     SPAFYPILSGRDNLRVFARTAGNEDARTIEGVLQQVGLSSRAQDKVKTYSLGMKQRLA
                     IAAVLLNHPQIIFLDEPTNGLDPAGTVEIRELIGQLGASGHTIFLSSHLLHEVEQVCN
                     EVAIINHGKMVAQGRVADLLKREASLLIEAEPLATLQAVVERFGATQQAIGPRTITVA
                     LQPERAPELMKALVIILMQTLLPLVAGTVALMGAVIGRSSVAGIVIGIAWFSIDSLLG
                     GLFPLASLSNAAMFIQARLTGMVMASNGSITPVRLPGELPGLLGLIPFVVVVFYLVVP
                     ITVAAIVFRKRDMLGAA"
     gene            1169..1933
                     /locus_tag="VGT44_15915"
     CDS             1169..1933
                     /locus_tag="VGT44_15915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582342.1"
                     /translation="MTTPLSDSQNQPRDTAFWAEEASVLKVERAPTGALNLNVEGHEV
                     LSPLQGFGQLWQKVYQARVKNAAVTPGEIISAWKENFSQFWPKGNRFFAPPMGIAPGA
                     VAILSFGVPSVPGGLGMISTGVFVLYADDESFTFMTPEGHLFASWITFSAYQEDDCTV
                     IQIHMLLRPNDPLYEMGMRLRVIPFFEDRFWRQTILRLMAHFNIDAPVKITATCIDKR
                     LQWSQAGNIWQNAAVRTVLYTAMSPLRRMRARLRNV"
     gene            complement(1941..>3024)
                     /locus_tag="VGT44_15920"
     CDS             complement(1941..>3024)
                     /locus_tag="VGT44_15920"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582343.1"
                     /translation="LSPNYIFLLVFRFLTAIGVGAEYTAINSAISEFIPPRHRGKING
                     LVMNFWPIGAMIAALVNLLFINLLAPEIGWRVGFAFGAIAALFVVWMRRAIPESPRWL
                     LSKGRHQEAEAVVRTIETRAGQTQHETLAAILVDARPAQPFFSQVYELITRFPGRLAL
                     GCLLDLSEAFGYYGMFTFLALAVLPAVHIAATQIPWFYFLGNVGALLGGLAMLSVIDR
                     LGRKITVPAFYTLAALSALLLAPATRTGSGLVVLLAFLLANFCATGAWTSAYPTFSEI
                     FPTHLRSTGVGLSVAVGRTGAIFSGPLLIALFQGPAGLTGALVMMAALWLVGALVMIP
                     WYFRGVEGARTSLENMVRFPTEVVVE"
BASE COUNT          635 a          913 c          928 g          548 t
ORIGIN      
        1 cgcgggaaag acgacgacga tccggatgct gctcggcctc attcgcccaa cacagggcgg
       61 cggacgcatt ctgggccacg atatcgagaa tgaacgcgcg gccatcctgc cccacgtcgg
      121 cgcacttgtc gaatcgcccg cgttctaccc catcctgtcg ggccgtgaca acctgcgagt
      181 gttcgcgcgc accgctggca acgaggacgc gcgcaccatc gagggcgtgc tacaacaggt
      241 gggcctgagc agccgcgcgc aggacaaggt caagacctac tcgctgggca tgaagcagcg
      301 cctggcgatc gccgccgtac tcttgaacca cccgcagatc atctttctgg acgagccgac
      361 caacggcctc gacccggctg gcacggtcga gatccgcgag ttgatcgggc agctcggcgc
      421 gagcggtcac accatcttcc tctcaagcca cctgctgcac gaggtggagc aggtctgcaa
      481 tgaagtggca atcatcaatc acggtaaaat ggtggcccag ggccgtgtcg ccgatttgct
      541 caagcgtgag gccagcctgc tgatcgaggc cgaaccgctc gcgacgttgc aggcggtggt
      601 cgagcgcttc ggcgcgacac aacaggccat cgggcctcgc accatcacag tcgcattgca
      661 gccggaacgc gcgcccgaac tgatgaaggc gctggtcatt atcctgatgc agacgctgct
      721 cccgcttgtt gctggcacgg ttgctctgat gggggccgtg atcgggcggt cgagtgtcgc
      781 ggggatcgtg atcggtattg cctggttcag catcgattcg ttgctgggag ggctgttccc
      841 cctggcgagc ctcagtaatg ccgcgatgtt tattcaggcg agactgaccg gcatggtgat
      901 ggcttctaac gggagcatca ctccagtacg tctgccgggc gagctgcccg ggctattggg
      961 cctgatcccc ttcgttgttg tcgtcttcta cctggttgtc cccatcaccg tagcggccat
     1021 cgtgttccgc aaacgtgata tgctgggagc ggcataagat gcgttcagct tgaggaaacg
     1081 tttaagaaac gttgcgcgga ctacactggc atgagaagat atcagacgtg ctaagccttt
     1141 cgacaaggag aacagaagga agagaacgat gacgacccca ttatccgact cacaaaatca
     1201 acctcgcgac acggcctttt gggcggagga ggcttcggtt ctcaaagttg agcgagcgcc
     1261 gaccggcgcg ctaaatctca acgtcgaggg tcacgaagta ctcagtccgc tgcaaggctt
     1321 cggccagctc tggcagaaag tctaccaggc gcgtgtgaaa aatgcggctg tgaccccggg
     1381 cgagattatc agcgcgtgga aggagaactt ctcccagttc tggccgaaag gtaatcgctt
     1441 ttttgcgccg cccatgggca ttgcccccgg cgcggtcgcc atactgagct ttggggtgcc
     1501 cagtgttcct ggcggcctgg gcatgatttc caccggtgtc ttcgtactct acgccgacga
     1561 tgaatccttc acgttcatga cgccggaagg ccacctgttc gccagctgga tcactttcag
     1621 cgcgtatcag gaggatgatt gcaccgtcat acagatccat atgctcctgc gccccaacga
     1681 tccactctat gaaatgggca tgcgcttgcg cgtgatccca ttctttgaag atcgtttctg
     1741 gcggcagacg atcctcaggt tgatggcgca cttcaacatc gacgcgcctg tcaagatcac
     1801 cgctacctgc atcgataaac gcctgcaatg gtcgcaggcc gggaacatct ggcagaacgc
     1861 ggctgtacgc acagtgctgt atacggccat gtccccgctg cgccggatgc gcgccaggct
     1921 gaggaatgtg taaggacgcc ctactcgacg acgacttctg tggggaagcg taccatattc
     1981 tccagagacg ttcttgcccc ctcaacccct cggaagtacc aggggatcat caccagtgcc
     2041 cccacaagcc agagagccgc catcatgacg agcgcgccgg tgagtccagc tggcccctgg
     2101 aacagcgcga tcagcaaggg tccgctgaag atggcgccgg tgcgaccaac cgccaccgat
     2161 aggccgacac ccgtcgagcg caggtgggta gggaaaatct cagagaacgt cggataggcg
     2221 ctggtccagg cccccgttgc gcagaagttc gccagcagaa aggcgagcaa gaccaccagg
     2281 ccgctccccg ttctcgttgc gggagccagg agcagtgcgg acagggctgc cagcgtgtag
     2341 aaagcaggca cggtgatctt gcgccccagg cgatcgatca ccgacaacat cgccaatcca
     2401 ccgagcagcg cgcccacgtt gccgaggaag tagaaccagg ggatctgcgt cgcggcgatg
     2461 tggactgctg gaaggacggc cagcgccagg aaggtgaaca tcccatagta gccgaaagct
     2521 tcggagaggt cgagcaggca tcccagcgcg agccgaccgg gaaagcgcgt gatgagttca
     2581 tatacctggc taaaaaacgg ctgagccggt ctcgcatcga ccagaatggc cgcgagcgtc
     2641 tcgtgctgcg tttgacccgc ccgtgtttcg atggtgcgca cgaccgcctc tgcttcctgg
     2701 tggcgtcctt tggacaacag ccagcgtgga gattcgggga tggcgcgccg catccagacg
     2761 acgaagaggg ccgcgatagc cccaaaggca aacccgacgc gccaaccgat ctcaggagct
     2821 aacaagttga tgaacagcag attcaccagg gcggcgatca tcgccccgat gggccagaaa
     2881 ttcatcacca gcccgttgat cttgccgcga tgcctgggcg ggatgaactc gctgatggcg
     2941 gaattgatgg cggtatactc ggctcccacg ccgatagcgg tcaggaagcg gaacaccagc
     3001 aaaaagatgt aattcggtga aagc
//
LOCUS       DASZAK010000907         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_521671, whole genome shotgun sequence.
ACCESSION   DASZAK010000907 DASZAK010000000
VERSION     DASZAK010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521671"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VGT44_15925"
     CDS             complement(<1..768)
                     /locus_tag="VGT44_15925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2582344.1"
                     /translation="MQLGVTFPQTEIGADPAAIRDYAQAIEGLGYDHIVAFDHVLGVD
                     ATHRPGWRGYTSKDMFHEPFVLFGYLAALTRLELVPAVIILPQRQAVLVAKQAAEVDV
                     LTGGKLRLGVGIGWNPAEYEALGMDFHTRGRTIEEQIEVMRLLWSREIVSFQGQSHTI
                     SEAGLNPLPARRSIPVWLGGSAEALLRRVARIGDGWFPQGKPDSQMRETLERLRSYIL
                     EAGRDPAAVGIEARMSAREGDLDEWVRQTDAWRKLGAT"
     gene            complement(814..1800)
                     /locus_tag="VGT44_15930"
     CDS             complement(814..1800)
                     /locus_tag="VGT44_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEV2582345.1"
                     /translation="MQLGGIHHVTAVTGNASRNVAFYTQALGMRLVKKTVNQDDTSAY
                     HLFYGDDVGRPGFDLTFFEWANIGRHQPGAGTISTIALGVNGRDALDWWSRRLDDYHV
                     DHDGIQAREVGGSDAIAYLAFRDPEGQRLELVDAVGKLDMTPWRGSPVPVEMAIHGFY
                     AVRLTQRSLEATARFLTETLGFRSAGAYQTAQQTPVTVFEVGPGGPGTEVHVEVDPTQ
                     PFRRFVGVGGVHHVAFRTPNEEEHRQWRERIARVNPTITPVIDRFYFRSIYFREPGGV
                     LFEIATDGPGFAVDEDVETLGERLSLPPFLESHRGEIEAHLHPIVPVELPVG"
BASE COUNT          430 a          662 c          556 g          401 t
ORIGIN      
        1 cgtcgcgcca agtttgcgcc atgcatccgt ctgtcgcacc cactcgtcga gatcgccttc
       61 tcgggcgctc atgcgggctt cgatgcctac tgcggcgggg tcgcggccag cttcaaggat
      121 gtaggagcgc aggcgttcga gcgtttcgcg catctggcta tccggtttcc cctgggggaa
      181 ccagccatct ccgatgcgcg cgacccggcg cagcagcgcc tcggcgcttc ctcccagcca
      241 gaccgggata gagcgccgcg caggtagcgg attgaggccc gcttccgaaa tggtatggct
      301 ctgtccctgg aagctgacga tctcgcggct ccagagcagc cgcatcactt cgatctgctc
      361 ttctatggtg cggccccgtg tgtgaaagtc catgcccagc gcctcgtact ccgcgggatt
      421 ccagccaatt cctacgccca ggcgcagctt accgccggta agcacatcaa cctccgccgc
      481 ctgctttgcg acaagcacag cctggcgctg cgggaggata atgaccgccg ggacaagctc
      541 caggcgcgtc agcgccgcga gatagccaaa cagcacaaac ggctcgtgaa acatatcttt
      601 gctggtatac cctcgccagc ctggacgatg cgtagcatct acgcccagca catggtcgaa
      661 ggcaacgatg tggtcgtacc ccaatccttc tatagcttgc gcgtaatcgc gaatggcggc
      721 tggatcagcc cctatctcgg tctgcggaaa cgtaactcca agctgcatgt ctcaatccct
      781 cgtgcttcat atggcgacgg caaactaccg tccctatcct acaggcaatt caaccggcac
      841 aatcgggtgc aaatgtgctt ctatctcgcc acggtgcgat tcgaggaagg gaggcagcga
      901 gagccgctcg ccgagcgtct cgacatcttc atcgacggca aagccggggc cgtcggttgc
      961 aatttcaaac agcacaccgc ctggctcgcg aaagtagatc gaacggaagt agaaacggtc
     1021 gatcaccggc gtgatggtcg gattgaccct ggctatgcgc tctctccact ggcggtgttc
     1081 ctcctcgttg ggagtgcgga aggccacgtg atgcacacca ccgaccccca cgaagcgccg
     1141 gaatggctgc gtaggatcaa cctccacgtg aacctccgtc ccagggccgc cggggccaac
     1201 ctcgaagacg gtcactggcg tctgctgtgc cgtttgatac gcgcccgcgc tgcgaaagcc
     1261 cagcgtctcg gttaaaaagc gggcagtcgc ttcgaggctg cgctgcgtga gcctgacggc
     1321 gtaaaaaccg tggatcgcca tctcgaccgg cacaggactg ccgcgccagg gagtcatatc
     1381 gagcttcccg acggcatcca ccagttcgag acgctgcccc tccggatcgc ggaacgcgag
     1441 ataggcaatg gcatctgagc cacccacctc gcgcgcctgg atgccgtcgt gatccacgtg
     1501 gtaatcatcg agtcgcctgc tccaccagtc gagcgcatcc cggccattca cgcccagcgc
     1561 aatcgtcgaa atcgtccccg cgccaggctg gtgccgcccg atattcgccc attcaaaaaa
     1621 tgtcagatca aagccaggac gacccacatc atctccataa aagaggtgat aggccgacgt
     1681 atcatcctgg ttcaccgtct tcttcaccag gcgcatccca agcgcctgcg tataaaacgc
     1741 cacgttgcgc gaagcattgc ccgtcacagc ggtgacgtga tgtattccac caagttgcat
     1801 attccagttc ctccagatca tcaaagataa tcaacacctt tacacttgat tcaacaaaca
     1861 ggacactgct cctatttcca tatagcacac cccgcccacc gggtggttat catgggaaaa
     1921 acgtctgact cgcggcgtaa ttaggataga tgcttcgctg cgctcagcat gacatgtcta
     1981 tttacagagt accattcccg ccagttttat gttagactgg tgtcctgtca agacattaga
     2041 aggtcgaga
//
LOCUS       DASZAK010000908         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_663204, whole genome shotgun sequence.
ACCESSION   DASZAK010000908 DASZAK010000000
VERSION     DASZAK010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663204"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VGT44_15935"
     CDS             <1..994
                     /locus_tag="VGT44_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEV2582346.1"
                     /translation="YQRVNGPILLVLIVAMGIGPLLAWRRTSLSALWRHLGIPALAAA
                     LCAAILPIAGVRDIWANMAFAVCAFTAGAILYEIWRGVRVQHSHGENYLVALFTLIDR
                     YRQRYGGYIVHLGFILLAIGVIGSNFFQSSQDAVLTPGQEINVAGFKLVYFGNVDLKY
                     PDVETITSQLQVWRDGQLQGYIYPGRSFYPHFDNQPASSISITTYNLTDLYVFLADWN
                     GAGQATIRVFVNPLVPLVWLGGLTMLYGGIVCWWPTRPKRRAKTITQPASVGAGVAAE
                     GGGDARVALVGGHDSHVSEADARVALVGGHDPRLSDADEIATTAQQRTGEEATL"
     gene            991..1533
                     /locus_tag="VGT44_15940"
     CDS             991..1533
                     /locus_tag="VGT44_15940"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HEV2582347.1"
                     /translation="MTRRRTLLAGFAAIVIVALLAAIWWFMLFATPPQETLDQRVYDV
                     ASQLKCPVCQGESVANSPALISQQMRALIRQQLQSGMTEQQSIQYFRSRYGDRILWSP
                     PQQGFTLLAWLVPPAVLLLGALLVFFVLRNWGRTRRAAQTRSSEELVDQHELEQFQAQ
                     YERELAAEDALFARQSKEAS"
     gene            1533..1928
                     /locus_tag="VGT44_15945"
     CDS             1533..1928
                     /locus_tag="VGT44_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582348.1"
                     /translation="MPQLSFLPWLVALILCVPALFIVLYPLYLPYQRMLAGAHPESDE
                     EHQAALPSQIEHEQAARSALTEVELDYQLGNLAEPDYRSLRERYLLRAYTAHKSIQAH
                     EQQLDAQIEDRLRQMRGTEEYQDRDNAAE"
     gene            1936..3444
                     /locus_tag="VGT44_15950"
     CDS             1936..3444
                     /locus_tag="VGT44_15950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582349.1"
                     /translation="MNKKISGAAMRAVSGTTVGEVADATMDKGRPQGSRHASRQTPLR
                     SPSSLLLWRTSILAILLLALLLAFPLTASAQTLPTVASSGRIYGQLRDGTKRNAPVAG
                     QSVTLQGAQGGNTGDLETTTTDAHGMYSFGGLSTDKTISYAVYTLYQKAQYVSGLVDL
                     SSRPQQQVDLTVYDATTSVAKIAIVQANILIDKADPQHGLLTITESFYFENLGLTTYV
                     GSVAANGQKPNALLFSLPPGAQQVSLKAGFDGYQQAQVNNGFASTAAVPPGLSQFAFS
                     FQVSYSATHYDFSYTVLYPIVSLSVLLPLDYHASSAALLPQGTVNTNGQVYQQLSAQQ
                     LLANARVHVELDGLPVTTTASGRPVLGLDLSWLIWIVLAMLAIVGITWYLATRARRST
                     GKTRTVRPPTRASSSRKAPVQAKATNGAKAAREKQAAPSEEEQTLQVSLLAELLALDK
                     AYEAGTIKKAAYDERRSRLKARLRAVMSAEQDEQTANASGTKKASKSGAKKA"
     gene            3455..>3773
                     /locus_tag="VGT44_15955"
     CDS             3455..>3773
                     /locus_tag="VGT44_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2582350.1"
                     /translation="MNAPYLEVIGLKKSFGLKPALRGIDLRVEQGQRMALLGANGAGK
                     TTLLRTLAGLAKPDVGSARISGLDLRQDAQQARSLVGFVAHQPYIYEELTALENLRFF
                     GRMY"
BASE COUNT          750 a         1247 c         1087 g          689 t
ORIGIN      
        1 ctaccagcgc gtcaacgggc cgattttgct cgtcctgatt gtggcgatgg gcattggccc
       61 gctgctggcg tggcggcgca cctcgctgtc cgcgctctgg cgtcacctgg gcatacccgc
      121 gctggccgcc gcgctctgcg ccgctatcct gccgattgcc ggtgtgcgcg acatatgggc
      181 caacatggcc ttcgccgtct gtgccttcac cgccggagca atcctttatg aaatctggcg
      241 cggtgtgcgc gtgcagcata gtcacggcga aaactacctc gtcgcgctct tcacgctgat
      301 cgatcgctac cggcagcgct acggcggcta tatcgtccac ctgggcttca ttctgctggc
      361 catcggcgtc atcggctcca actttttcca gtcgtcgcag gatgccgtcc tcactcccgg
      421 acaggagatc aatgttgctg gcttcaaact ggtgtatttc ggcaacgtcg atctgaaata
      481 ccccgatgta gagaccatca cctcgcagct ccaggtctgg cgcgatgggc agttgcaggg
      541 ctatatctat ccgggccgct cgttctaccc gcacttcgat aatcaaccgg ccagcagcat
      601 ctctattacc acctacaatc tgaccgacct gtacgtcttc ctggccgact ggaacggcgc
      661 gggccaggca accatccgcg tcttcgtcaa cccgctcgtg ccgctcgtct ggctcggcgg
      721 cctcactatg ctctacggcg gcatcgtctg ctggtggccc acccgcccca aacgccgcgc
      781 caagaccatc acccagcccg catccgtagg ggcgggggtg gcggcggagg ggggtgggga
      841 cgctcgcgtc gccctcgtcg gcggtcacga ttcgcacgta tccgaagctg acgctcgcgt
      901 cgccctcgtc ggcggtcacg acccgcgttt atccgatgcc gacgaaatcg cgacgacagc
      961 ccaacagcgt accggagagg aggcgaccct atgaccaggc ggcgcacact cctcgccgga
     1021 tttgcggcaa ttgtaatagt cgcgctgttg gcggccatct ggtggttcat gctcttcgcg
     1081 acaccgcccc aggagacact tgaccagcgt gtctacgacg tggcctcgca gttgaaatgc
     1141 cccgtctgcc agggagagtc ggtggcgaat tcgcccgccc tgatctcaca gcagatgcgc
     1201 gccctcatcc gccagcagtt acaatcgggt atgactgagc aacagagcat ccagtatttt
     1261 cgctcgcgtt acggtgatcg catcctctgg tcgccgccac agcagggctt tacgctgcta
     1321 gcatggctcg tgccgcccgc cgtcctgctg ctgggcgctc tgctcgtctt cttcgtgctg
     1381 cgcaactggg gcaggacgcg ccgcgccgct caaaccagat caagcgaaga gcttgtcgac
     1441 cagcacgaac tggagcagtt ccaggcacag tacgaacgcg aattggccgc cgaagatgcc
     1501 cttttcgcgc gccagagtaa ggaggcgagt tgatgccgca gttgtcattt cttccctggc
     1561 tggtcgcgct gattttatgc gttcccgcgc tgttcatcgt cctctacccg ctctacctgc
     1621 cataccagcg aatgctggca ggcgcacacc ccgaatcgga cgaagagcat caagccgcgc
     1681 tgccgtcaca aatcgaacac gagcaagccg cccgcagcgc gctcaccgaa gtcgaactcg
     1741 attaccagct aggcaacctc gccgagccag actatcgctc cctgcgcgaa cgctacctgc
     1801 tccgcgccta caccgcccac aaatccatcc aggcccacga acaacagctc gacgcccaga
     1861 tcgaagaccg cctgcgccag atgcgaggga cggaagagta ccaggatcga gataatgcag
     1921 cagagtaaga ttagtgtgaa taagaaaata tccggcgctg ctatgcgagc ggtctccggc
     1981 actactgtgg gcgaagtagc cgacgctacc atggataaag ggcgaccgca agggtctcgc
     2041 cacgctagca ggcagacacc cttgcggtcg ccctcgtcct tactgctctg gcggacgagc
     2101 atactggcga tcctcttgct cgccctctta ctcgccttcc ccctgaccgc gtcggcgcaa
     2161 accttaccca ccgtggcaag cagcggtcgc atctatggac agttgcgcga tggcacgaag
     2221 cgcaacgcgc ccgtcgcggg gcagagcgtc acgttgcagg gagcgcaggg cggcaacacc
     2281 ggcgacctgg aaacgaccac gaccgacgca cacggcatgt actcttttgg cggcctctct
     2341 accgacaaaa ccatcagcta cgccgtgtat acgctctatc agaaggcgca atacgtcagt
     2401 ggcctggtcg atttgagcag caggccccag cagcaggtcg acctgacggt ctacgatgcc
     2461 acgaccagcg tcgcgaaaat cgccatcgtg caggccaaca tcttgatcga taaggcagac
     2521 ccgcagcatg gcctgctgac catcaccgaa agcttctatt tcgagaacct gggactgacc
     2581 acctatgtcg gctcggtggc ggccaacggg caaaagccca acgcgctgct gttttcgctg
     2641 ccgccgggcg cgcagcaggt ctcgctcaaa gccggtttcg atggctacca gcaggcgcag
     2701 gtcaataacg gcttcgcctc caccgccgcc gttccgcccg gcctgtcgca gtttgccttc
     2761 tcgttccagg tatcctacag cgcgacgcac tacgatttca gctatacggt tctctatcca
     2821 atcgtcagcc tgtccgtgct gctgccgttg gactatcacg ccagttccgc cgcgcttctg
     2881 ccccagggaa cagtcaatac caatggccag gtataccagc aattatccgc gcagcaacta
     2941 ctggccaacg cgcgggtcca tgtcgagctg gacggtttgc cagtcacaac gaccgcaagc
     3001 ggccgaccgg ttctgggcct ggacctgtcc tggctgatct ggattgtgct ggccatgctg
     3061 gccatcgtgg gcatcacctg gtacctggca acacgtgcgc ggcgcagtac aggcaagacg
     3121 cgcacagtcc gcccgccgac acgagccagc tcgtcgcgca aagcacccgt ccaggcaaag
     3181 gcgaccaatg gcgcaaaagc tgcgcgggaa aagcaggccg cgccgtcaga agaggagcag
     3241 acgttacagg tatcgctgct cgcggaactg ctggcactcg acaaagcgta tgaagctggt
     3301 acaataaaga aagctgccta tgacgaacgt cgctcccgtc tcaaggctcg cctgcgcgcc
     3361 gtgatgagcg cggagcagga cgagcaaacg gcaaatgcta gcggcacaaa gaaagcgtcg
     3421 aagagtggcg ctaaaaaggc ataaaagagg catcgtgaac gcaccatacc tggaagtgat
     3481 cggcttaaag aagagtttcg ggctgaaacc cgccctgcgc ggcatcgatc tgcgcgtaga
     3541 gcagggccag cgcatggctc tactcggcgc gaacggcgct ggcaagacaa ccctgctgcg
     3601 caccctggca gggctggcaa agcctgacgt tggatcagcg cgcatcagcg gcctggatct
     3661 ccgccaggat gcgcagcagg cgcgttccct ggtaggattt gtcgcgcacc agccctatat
     3721 ctacgaagaa ctgaccgccc tggagaacct gcgtttcttc ggacgcatgt acg
//
LOCUS       DASZAK010000909         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_484726, whole genome shotgun sequence.
ACCESSION   DASZAK010000909 DASZAK010000000
VERSION     DASZAK010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484726"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..3167)
                     /locus_tag="VGT44_15960"
     CDS             complement(123..3167)
                     /locus_tag="VGT44_15960"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEV2582351.1"
                     /translation="MGKQSPIRVALDLETTGLHAEQDTILEVAAIKFRDETILDAMET
                     LVAPGRPIPFRVQRLTGITPQSILNAPAFESIAGQLQQFIGDYPIVGHSIPFDAGFLR
                     RRGLMRTNPLIDTFELAAVLLPSLPGYALDQVARALGIAIAPGRHRAMVDTDLAMKVF
                     LALHRLLHTVDTPILKDLADLDAPHSWPLLGFLRHEWHVRMEEDGLRSSSLRGSLGDR
                     LAAQLGMNPRILSFAVAQQSHLPAEPLAVEPPGDQPVEPRQGGNDAVSSAIRQAFDRR
                     TPLLLEVTVGANDYSPALKPALEWVSSQADAADGRCLVIAVANAQNARRLYDTTLPRL
                     QASLKIELPSAFLAERDGYLCLHRWFSPAQRRTSGELSAEQARGIAKLSIWAQQTLTG
                     ERGELTLLPQEITAWERISSGVEHVALADEQGATPYANCLYRKKGYCFSYRAEERVRQ
                     ARVIVTTHAGLLDDLTSSRSLLKQAGHYLVLDADLLEEECARWSGEEFHATRLHDLLH
                     TIGAELPDGRYKGLLALAAPSLNEHGPGGLATTPAIDGAALDARLLSWFQTLRQAHTA
                     IDTLFSALGHLLQEGTNGGGARDKNKPENAQRGGGGRGGERTDQPLRLSAQMRNLASW
                     MEVDRAWQQLSQRLRAVVTLIEEAQKQLLAAKRGRPYSNQEGDDSESVAWELAALARR
                     LVEQKKMAHNAFALGTEPDVVDSTPSLVYWLRVPPPPAQPRASEAVQPPPAQNGESAP
                     VLHAQIAQPASFIKRHLLSPGTGAVLAGIALSVDHQFSFYRGRYNLEPDTCPALSVTT
                     EFHEQTLLYLPDDVPEPNAPQYQRHLDDASINLATMLDGQVVVLFTSYSALRSSYAAV
                     KPLLESRNILTLGHGIDGSPRQLWQMFQQQERIVLLGTGSFWDGSEEVNRPPACILVS
                     RLPMPVLNDPPVAAMAEQYSDQLHQVTVPLASLRVRRALNRLAWSGTGRNAVILFDRR
                     VLSKEYGATVLHTLPRCSQRQGAVSHMPEFILDWLTTTGARD"
BASE COUNT          655 a          975 c         1016 g          628 t
ORIGIN      
        1 gtacctcgca gacagatggt tgggccgcac gaggcgtcca ggcgctacac ttgctcatca
       61 ataatatcgg ctattataac atagccgata gatacttttc tacattttta cacagaacct
      121 cgtcaatcac gcgcgccggt ggtggtgagc cagtcgagga taaattcggg catgtgtgag
      181 accgcgccct gccgctgcga gcagcgcggc agcgtatgca gaacggttgc accgtattct
      241 ttcgagagga cgcggcgatc aaagagaatc acagcgttgc gcccggtgcc actccaggct
      301 agccggttga gggcgcggcg cacacgcagc gaggccagtg gaaccgtgac ctggtggagc
      361 tgatcggagt attgctcggc catcgccgcc acgggcgggt cgttcagcac gggcatcggc
      421 agccgcgaca cgagaatgca ggcgggcggg cggttgacct cctcgctgcc atcccagaag
      481 ctgcccgtcc ccagcaggac gatacgttcc tgctgctgga acatctgcca gagctggcgc
      541 ggcgagccat caatgccatg ccccagcgtc aagatgttgc gcgactccaa aagcggcttg
      601 acggcggcat acgaactgcg caaggcagaa taggaggtaa agagaaccac gacctgccca
      661 tccaacatcg tcgcaaggtt gatactggca tcgtccaggt ggcgctggta ctggggcgca
      721 ttcggctctg gcacatcgtc gggcaggtag agtagggttt gttcgtgaaa ctcggtggtg
      781 acggagagtg cgggacaggt atcgggttcc aggttatagc gtccacgata aaaggagaat
      841 tggtgatcca cggaaagggc aatgccggcc agcacggcac cggttccagg tgagaggaga
      901 tgacgcttga tgaacgaggc aggctgcgct atttgcgcgt ggaggactgg cgcgctctca
      961 ccgttctgcg ccggtggcgg ctggaccgct tcagatgcgc gcggctgtgc cggtggaggc
     1021 gggacacgca gccagtagac cagggaaggt gtggaatcta caacgtccgg ctcggtcccg
     1081 agagcgaagg cgttgtgtgc catcttcttc tgctcaacca gccgacgcgc cagcgccgcc
     1141 agttcccacg cgaccgattc gctgtcgtcg ccctcctggt tagaataggg ccgaccgcgc
     1201 tttgctgcca acagctgctt ctgagcctcc tcgattagtg ttaccacggc tcgcaagcgc
     1261 tgcgagagct gttgccaggc gcggtctacc tccatccagc tcgccaggtt gcgcatctgg
     1321 gcgctcaaac gcagtggctg gtcggttcgt tctccaccgc gcccgccacc tccgcgctgc
     1381 gcgttctccg gcttgttttt gtcgcgcgcg ccgccgccat tagtgccttc ttggagcaaa
     1441 tgaccaagcg cactaaacag ggtatcgatg gccgtatgag cctggcgtaa tgtttgaaac
     1501 cacgacagca agcgcgcatc cagcgccgcc ccgtcgatcg ctggcgtggt cgcaagtcct
     1561 cccggaccat gttcgttcag gctgggggcg gccagagcca gcaatccctt gtagcgtcca
     1621 tcgggcagtt cagcgccgat ggtatgcagc agatcgtgca gccgtgtggc atggaattcc
     1681 tcgccactcc aacgggcgca ttcttcttcg agcaagtccg cgtcgagtac caggtaatgt
     1741 ccggcctgct tcagcaagga gcgcgacgag gtcaggtcat ccagcagacc ggcgtgtgtg
     1801 gtgacaatca ctcgcgcctg cctgacacgc tcttccgcgc gataggaaaa acaatagcct
     1861 ttcttgcgat agagacagtt cgcgtaaggt gttgcgccct gctcatcagc cagcgcgaca
     1921 tgctcaacgc cggaggaaat gcgttcccac gcggtgattt cctgtggcaa cagagtcaac
     1981 tcccctcgct caccggtgag ggtctgctgc gcccaaatcg acagtttcgc gatgccgcgc
     2041 gcctgctccg cgctcagctc gccgctggtg cggcgctgcg ccggactgaa ccagcgatgc
     2101 aggcagaggt atccatcccg ttcggcaaga aacgcactgg gcagttctat cttcaatgat
     2161 gcctgcaaac gcggcagcgt tgtatcatac agccggcgcg cgttttgcgc attggcgacc
     2221 gcgattacca ggcagcgacc atcggcagca tcagcctggg atgacaccca ttccagggca
     2281 ggcttgagcg ccgggctgta gtcatttgca ccaaccgtga cctctaagag cagtggagtg
     2341 cgccggtcaa acgcctgcct gatagcgctg gatacagcat cattcccgcc ctgacgcggc
     2401 tcaaccggtt gatcgcccgg cggctcaaca gccaaaggtt cagcaggtag atgtgattgc
     2461 tgggcaacgg caaatgagag gatgcggggg ttcatgccaa gctgtgcggc caggcgatct
     2521 cccaggctgc cacgcaagct actcgaccgc agaccatctt cctccatacg cacatgccat
     2581 tcgtgccgga gaaagccgag cagcggccac gagtgcggcg catccaggtc cgccaggtcc
     2641 ttgagaatgg gtgtatcgac ggtatgaagc agccgatgca gcgcgagaaa gaccttcata
     2701 gccaggtcgg tatccaccat agcgcggtgc cggcccggcg caatggctat ccccagcgcg
     2761 cgcgcaacct ggtcgagcgc gtagccgggc agggaaggca agaggaccgc cgctaactcg
     2821 aacgtatcga tgaggggatt cgtgcgcatc aagccgcgcc gtcgtaggaa cccggcatcg
     2881 aatggaatgc tatgaccaac gatgggataa tcgccgatga attgttgcag ttgcccggcg
     2941 atcgattcaa acgcgggcgc attgaggatg ctctgtggcg tgataccggt cagtcgctgc
     3001 acgcgaaaag gaatgggacg ccctggcgcg accagggtct ccatcgcatc caggatggtc
     3061 tcatcacgaa acttgattgc agctacttca agaattgtgt cttgttcggc atgcaatccc
     3121 gtcgtttcga ggtcaagcgc cacgcggatc ggcgactgtt ttcccataca cgcacatatt
     3181 ccttgttttc ttttgacgag cgtcgatgtt ggttaagagt ggcctatgtc tcgtcccggc
     3241 gcaaatgatg cgtaggggcg caatgaattg cgcc
//
LOCUS       DASZAK010000910         2323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_379402, whole genome shotgun sequence.
ACCESSION   DASZAK010000910 DASZAK010000000
VERSION     DASZAK010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379402"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VGT44_15965"
     CDS             complement(<1..208)
                     /locus_tag="VGT44_15965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582352.1"
                     /translation="MSQFKAKDLYQQLGQAQDQKQHGHARLIQAETLLKSVHTPLPTR
                     LVDARKEAEYRTRRIRAIPSHKSRH"
     gene            complement(270..1982)
                     /locus_tag="VGT44_15970"
     CDS             complement(270..1982)
                     /locus_tag="VGT44_15970"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEV2582353.1"
                     /translation="MKANIINDTCRKGFRYQLWLACLLCSILIGCGVASPSVLARSGT
                     IFPSSCAVKLPASVGYWPMNTNAFQGVFQFTGQANTKNAGNPSIGGANLEWSWADIEQ
                     VEGQYNWSKVDRDIGLWTSQGKQVILRFATAGQLSWSGSVDGSYTPPWVFDTYGVPRV
                     TEINGTVFPLYWNPIYLQKLADFVRTAAARYDTNPYVAAVQIGIGQGGETEPDGSASQ
                     NPHQLQLWQRYGYTNALWWDTIKRIVAIYEAAWTHTPLALMTTSTFLKYDDKYFNRTL
                     VERYAVQQGLWLQINSLDDAMPTSLLADGVNHLTTTVEEQRQSAQQSGYPAIHDVKHA
                     IALGARYALVFASDLANPSNADALAYAQQQITAPVMQDCSGKNLALSVVGRVTPVPGS
                     SGKPDTYAQLFDGATGSASAGQSPLQSVATWTLDVWLNPTTLPQTEAIAVMDGAESGG
                     IGLGIAAGAQGGSGSELVAYFPGVGWVDSGYAFPAPRQWYHVVVTRDGTTVRFYVNGA
                     QTSATSALSPLTVASHFSAGSGFDLSTNAPTHFFTGAIDDVAVYASALGPAKVRAHMN
                     DQPL"
BASE COUNT          501 a          731 c          599 g          492 t
ORIGIN      
        1 cgtgacggct cttatgggaa gggatggctc tgatgcgacg tgttctgtat tccgcctcct
       61 ttctcgcgtc cactaatcga gtcggcagcg gtgtatgtac agatttcagc agcgtttcag
      121 cctgaatcag cctggcatgt ccatgctgtt tctgatcctg tgcctgtcca agttgctgat
      181 acaggtcttt tgctttaaat tgcgacaaaa cgcccccacc ttcaacacta tctacatatc
      241 cctattgtta gtccctgccc ctcgacggac tacaatggct gatcgttcat atgcgcccgt
      301 acttttgcag ggccgagcgc gctggcatac acggctacgt cgtcgattgc gccggtgaag
      361 aaatgggttg gcgcattcgt cgagaggtca aaaccgctgc cggcgctaaa atgggacgcg
      421 accgtgagtg gactcaacgc acttgtggcc gacgtctgcg ccccgttgac gtaaaatctg
      481 accgtcgtgc cgtcccgcgt gaccacaacg tgataccact ggcgaggcgc cggaaaggca
      541 tagccgctat caacccaccc gacgccgggg aagtaggcca ccagttcgga gccgctgcca
      601 ccttgcgcgc ccgcggcaat gccaagcccg attccgccgc tctcagcccc atccatcaca
      661 gcgatggctt ccgtctgggg cagcgtcgtc ggattcaacc agacatcgag cgtccaggtt
      721 gccacgctct gcaatggtga ctgtccggcg gaggcgctgc cggtcgcgcc atcaaacaat
      781 tgcgcgtagg tgtccggttt gccgctcgag cctggcaccg gtgtcacacg ccccacgacc
      841 gaaagcgcca ggttcttgcc ggaacaatcc tgcataacag gcgccgtaat ctgctgctgg
      901 gcataggcca gggcatcggc attcgacggg ttcgccaggt cgctggcgaa aaccagcgca
      961 tagcgcgcgc cgagcgcgat agcgtgcttc acgtcgtgaa tcgccggata cccgctctgc
     1021 tgcgccgatt gccgctgctc ctcgaccgtc gtcgtcaaat ggttcacgcc atccgccagc
     1081 aggctggtcg gcatcgcatc gtcgagcgaa ttgatctgca gccagagacc ctgctgcacc
     1141 gcgtatcgct ccaccagtgt ccggttgaaa tacttatcat cgtacttcag aaatgtcgat
     1201 gtcgtcatca acgcaagcgg cgtatgcgtc caggccgctt cgtatatcgc aacaatgcgt
     1261 ttgatcgtgt cccaccacag cgcgttcgta tacccatagc gctgccagag ctgcaactgg
     1321 tgcggattct ggctcgcgga cccgtcaggc tccgtttcgc caccctggcc aataccgatt
     1381 tgcacggcag ccacgtaggg attcgtgtcg taacgcgccg ccgccgtccg gacgaaatcg
     1441 gccagctttt gcaggtagat cggattccag tagagcggaa acaccgtgcc gttgatttca
     1501 gtcacacgcg gcacaccata ggtatcgaag acccagggcg gcgtgtaaga gccatcaacc
     1561 gagccactcc aggagagttg ccctgcggta gcgaaacgca aaatcacctg cttgccctgc
     1621 gaggtccaca ggccgatatc gcgatcaacc ttcgaccagt tatattgccc ttcgacctgc
     1681 tcaatatcag cccagctcca ctcaaggttt gccccaccaa tagacggatt accagcattt
     1741 tttgtattcg cctggcccgt aaactgaaag acgccctgaa aggcgttcgt attcatcggc
     1801 cagtagccga cgctcgcggg cagcttcacc gcacatgatg acggaaatat cgttcctgat
     1861 cgagccagga cagacggtga ggccaccccg catccaatca agatcgaaca cagcaaacag
     1921 gccagccaga gctgatatcg aaatcctttc cggcaggtgt catttatgat attcgccttc
     1981 attccatctc ctcgcatcgg caataatttc gctacatcca tcttccatat cgacacatcg
     2041 acatctttct ttagcaacca tttgacctgt aaggccgcaa gaatcaactt ccaatagtaa
     2101 acaggttacg ccccgtaggt gccgatttat cgcgcacacc gccgattcat cggcccggat
     2161 gcctgccgtt cacgcccatg gtagccgatg aatcggcggt gtgcgcgata aatcggcacc
     2221 tacgggaaat tcatcattgc actcatgcca gggctttacg aacagttatc caccccgtaa
     2281 atacacaatc gagtcagccc ccgaatacac aacatgaaaa tcg
//
LOCUS       DASZAK010000911         4714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_698465, whole genome shotgun sequence.
ACCESSION   DASZAK010000911 DASZAK010000000
VERSION     DASZAK010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698465"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1388
                     /gene="murC"
                     /locus_tag="VGT44_15975"
     CDS             <1..1388
                     /gene="murC"
                     /locus_tag="VGT44_15975"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV2582354.1"
                     /translation="LMQKGVPVAIGHSPEHLANADALIYVPAVVAFNPDNPELAAAKA
                     SGMLVMTWQEMLGTWMQGKCVLSVSGVHGKGTTTAMLALMLVNAGLDPTCEIGAIVPG
                     FGVNYRIGKSQYFVNEADEFNNNFWHYHPRLAIVTSIEFEHPEFFANYATFLEAFEHF
                     IRGMDMHGTWPFAPTLVLNADSPGCLELRERLGDWAGRTLWYAVEGLSHAEATTTGTM
                     PSYANVLPDYVAFDVKLEGETSFRVKSQERGEKAFPTDKVIQLQLPGVHNIQNALAAL
                     TAARVIGIKDEVIIRTLENFSGIGRRFEIRHQGPLEIDGVTKDVILIDDYAHHPTAIT
                     ATLEAARKRFPGRRLIAVYQPHMYSRTKTFFEQFLTAFDSADVVIIADIFPGREHDTG
                     TVHARELVEAIHGRGRDTSGPSTTQVIYGGSVQDTAKLLTNILRSGDLAIIMGAGDIY
                     IVTEKILLHEM"
     gene            1398..2426
                     /gene="murB"
                     /locus_tag="VGT44_15980"
     CDS             1398..2426
                     /gene="murB"
                     /locus_tag="VGT44_15980"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV2582355.1"
                     /translation="MTFDAETIYTALLPHFKQRVRRDEPMARHCTFGVGGPADVWVSL
                     DTSRELMDLVRLCIEQQCPLLLAGNGTNILYADAGVRGIVARIALDTYTIEDHGDGTA
                     LLVAEAGVSWPRLLNELAVRGWGGLEFGPGIPGTLGGGVISNAGAHNSDLGEVLEWIE
                     VLDARSASTSQNGMSVPMKRRYLHDEIDLGYRHSRFRNDRRINFDARGYPIVPQRGLI
                     EPSEIILELGIRLRHEDPEKLRTTIEAYKQHRKRTQPPQKSAGSVFKNPPGDYAGRLI
                     DQAGLKGTVRGKAQISKRHANFIVNLGGASAADVVALIVEARNRVHERFGIDLELEVE
                     MRGEWGTL"
     gene            2423..3565
                     /locus_tag="VGT44_15985"
     CDS             2423..3565
                     /locus_tag="VGT44_15985"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEV2582356.1"
                     /translation="MTENKKPKEQIAFDYSWHTSSHPSSQGKEPGRKDRSRREEQFPA
                     QINVNTRKRIVTTPQEIVEPASWADRQARQRRSQAEPRTRSRFVKRTLAHTGVRAPEG
                     QVRLVRPLPQPTHATHMPVRSGRQHARRGSFWRRMLTIFTLLVMGIVGGSFALSSANF
                     RVQQIDIMGTQNSKLVDSIQHMGIQGQNIFLVDVTDMTTRIEALPTVASANLTKQLPN
                     LVIINVVERTPVLLWQTAEGTYSVDSKGVVIASASDTTGADHLMTVVDTRVGEMQQIH
                     PGALLNAPDIAFATQAFTQLQQLREITSFTLRFDVVPRQGGRGSFIVVSSKGWLAYLG
                     GSEDTNPLNNRLIELQQILNLAQQKQLNLATIDLRFGLRPVYTLKA"
     gene            3650..>4714
                     /gene="ftsA"
                     /locus_tag="VGT44_15990"
     CDS             3650..>4714
                     /gene="ftsA"
                     /locus_tag="VGT44_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV2582357.1"
                     /translation="MQERVIVGIDVGTTKICVLVGELDRDWKLNIVGVGTCPSQGLRR
                     GVVVNIEETVTSIAAALDRAERLSGKTITTAYVGIAGSHIDSENSKGFVAISPSHRDI
                     MQNDISRAIEVARAIAIPANREVIHVIPRGYVVDGQEGIKNPIGMSGFRLEVETHIIT
                     GAVSSIHNLIKCVHKARVEIEDLVLEPLASSEAVLSDGETDLGVALVDIGGGTTDVAV
                     FTDGAIWHSVVLPIGGNLITSDIAIGLRLPFAVAEELKVTYGHCDPSTIADDDMIDLS
                     HYLPDCNDLVPRKLLAEIVQARVEELFEMVHEEIKKSGCDGLLPAGLVVTGGGAELPG
                     ILDTAGQILDLPARLGSPLGL"
BASE COUNT         1150 a         1151 c         1390 g         1023 t
ORIGIN      
        1 tgctaatgca aaaaggcgtt ccggtagcga ttggtcacag ccccgaacac ctggcaaatg
       61 ctgatgcctt gatctatgta ccggcagttg tggccttcaa tcctgataat cccgagctgg
      121 ccgcagcaaa ggcaagtggc atgctggtga tgacatggca agagatgctg ggcacatgga
      181 tgcagggcaa gtgcgttctc tcggtcagcg gtgttcatgg caagggtact accacagcga
      241 tgctggcact gatgctcgtg aacgcggggc ttgacccaac gtgtgaaatc ggcgcaatcg
      301 taccgggctt tggcgtcaat tatcgcatag ggaaaagcca gtactttgtc aatgaagccg
      361 atgagttcaa taacaatttc tggcattatc acccgaggct ggcgattgtg acatcgattg
      421 agttcgagca tccagaattt ttcgccaact acgcgacctt tcttgaagcc tttgaacatt
      481 ttatacgtgg tatggatatg cacggaacct ggccttttgc cccaacgctc gttctgaatg
      541 ctgacagtcc gggatgcctg gaactgcgcg agcggctggg tgattgggcg ggacgcaccc
      601 tgtggtatgc agtggaagga ctttcgcacg cggaagcgac aacgactggc acgatgccgt
      661 catacgcaaa cgtcctgcca gactacgtgg cttttgacgt aaagcttgag ggtgagacaa
      721 gcttccgtgt caaatcgcag gaacgtgggg agaaagcgtt tccaacggat aaggtcatcc
      781 aactgcaatt accgggagta cacaacatcc agaatgctct ggctgcgctg actgcagcgc
      841 gcgtcatcgg gataaaggat gaagtgatca tccgcacgct ggaaaacttt agcggcatcg
      901 gcagacgttt cgaaattcgt catcaagggc cactagagat cgatggagtg acgaaggatg
      961 tcatcttgat tgatgactac gcgcaccacc caacggcgat tacagcaacc ctggaagcgg
     1021 ctcgcaagcg gtttccagga cggcggctta tcgcggttta tcaaccgcac atgtacagcc
     1081 gtacaaagac ttttttcgag caatttttga cggcctttga cagcgcagat gtggtgatta
     1141 tcgccgacat ctttccaggg cgcgagcacg atactggcac agtccatgct cgcgaactcg
     1201 ttgaagctat tcatggacgc gggcgcgata catcaggccc ctccacgacg caggtaatct
     1261 acggcggtag tgtgcaagac acagcaaagc tgttgaccaa catactgcgt tccggtgacc
     1321 tggcaatcat catgggtgca ggggacatct atattgtgac cgaaaaaatt ttattgcatg
     1381 aaatgtagga aaccattatg acgttcgatg ctgagacaat ctatacagca ctactgccac
     1441 attttaagca gcgcgtacgc cgcgatgagc caatggcacg ccattgcact ttcggtgtgg
     1501 gtggccccgc cgatgtgtgg gtttcgctcg acacgtcgag ggagcttatg gacctggtac
     1561 gcctgtgcat cgaacaacag tgccctctgc tgctggcagg caacggcaca aatatcctct
     1621 atgcagatgc tggagtgcgg ggtatcgttg cgcgaattgc cctcgatact tataccatcg
     1681 aagatcatgg agatggaacc gcacttctgg tggcagaagc aggcgtgagt tggccccgcc
     1741 ttttgaacga actcgcagtt cgtggctggg gtggattgga gttcggacca gggatacccg
     1801 ggacattggg cggaggtgtc atctccaacg cgggcgcgca taacagcgac cttggtgagg
     1861 tgctggaatg gatcgaggta ttggatgcga ggagcgcatc aacatctcaa aatggtatgt
     1921 cggtcccaat gaagcgacgt tacctgcacg acgaaattga tttgggttac cggcacagcc
     1981 gattccgtaa tgatcgacgt atcaacttcg atgcgcgggg atatccaatc gtccctcaac
     2041 gagggctgat tgagccgagt gaaatcatct tggagttagg gattcgtctc cggcatgaag
     2101 atcccgagaa gttacgcaca accattgaag cgtataagca gcatcgcaag aggacgcagc
     2161 cgcctcagaa gagcgcaggc tcggtcttca aaaatccgcc tggtgattat gccgggcgac
     2221 tgattgacca ggcagggctg aaaggaacgg tccgcggcaa ggcgcaaatc tctaagcgtc
     2281 acgccaactt cattgtcaac ttaggaggag ccagcgcggc cgacgtggtg gcgctcattg
     2341 tagaagctcg caaccgcgta cacgagcggt ttggaataga cctggagtta gaggtcgaga
     2401 tgcgaggcga atgggggacg ttatgactga gaacaaaaag ccaaaagagc agattgcttt
     2461 cgattatagt tggcacacat cgtctcatcc ttccagccag ggcaaagagc caggcagaaa
     2521 agacagatcg agaagagaag agcaatttcc ggcccaaatc aacgtaaaca cgcgcaaacg
     2581 cattgtcacg acgccgcagg agatcgtgga gccagcaagt tgggctgatc gacaggcccg
     2641 ccagcgccgc tcacaggcag agccgcgtac cagaagcagg tttgtgaagc gcacgcttgc
     2701 tcatacagga gtacgcgccc ctgaagggca ggtgcgactt gttcgacctt taccacagcc
     2761 gactcacgcg acgcatatgc cggtacgcag tgggcgacag cacgcgcggc gtggaagttt
     2821 ctggcgacga atgctgacta tctttacgct gctggtgatg ggtattgtgg ggggaagttt
     2881 cgcgctgagc agcgcgaact ttcgagtaca gcagattgat attatgggaa cgcaaaacag
     2941 caagctggta gacagcatcc agcatatggg tatccagggg cagaacattt ttctagtgga
     3001 tgtaacagat atgaccacac gtattgaggc attaccaaca gtggcctctg caaatctgac
     3061 aaagcagttg cctaacctgg tgatcataaa tgtggtggag agaacgcctg tattactctg
     3121 gcagacagcg gaggggactt acagtgtgga tagcaaggga gtagtgatag cgtctgccag
     3181 tgacaccaca ggcgcggatc atttgatgac agtcgtagac acgcgcgtgg gagagatgca
     3241 gcagattcat cctggagctt tgttgaacgc gccagacatt gcattcgcaa cgcaggcatt
     3301 tacacagttg cagcaattgc gagagattac ctcgttcacg ctgcgctttg atgttgtacc
     3361 caggcaaggg gggcgtggtt cttttatcgt tgtgagctcg aaaggctggt tagcctacct
     3421 gggggggtcg gaggatacca atccattgaa taaccggctg atcgaactcc aacagattct
     3481 taacctggcg cagcaaaaac agctcaatct ggcgacaatt gacctgcgct tcggcctgcg
     3541 ccctgtttac acgttgaagg cttaaggcca cgttacctgt agagatgtag tttgtagtgc
     3601 gcgggcatca ctcagggtgc ccagacaacc gcccaaggga gcgtgtatcg tgcaagagcg
     3661 ggtgatcgtc ggtatcgacg tcggaacaac aaaaatttgc gttcttgtcg gagaattgga
     3721 tcgcgactgg aagctgaata ttgttggcgt tggcacctgc ccttcacagg gtttgcgccg
     3781 cggggtggtg gtgaacatcg aggaaacagt cacttccatt gcagcggcac tagatcgtgc
     3841 agaacgcctt tcaggaaaaa ccataacaac cgcatacgtc gggatcgcgg gtagtcacat
     3901 cgattctgaa aatagtaagg gatttgtagc catctcaccc agccatcgag acatcatgca
     3961 gaatgacatc agccgtgcta tcgaggtcgc gcgtgctatc gcaattcccg cgaaccgcga
     4021 ggtaattcac gtgatcccgc gtggttatgt tgtagatgga caagaaggca tcaagaatcc
     4081 tatcggcatg tccggtttcc ggctagaagt agagacgcac atcattaccg gtgccgtgtc
     4141 atctattcac aacttaatta agtgcgtaca caaagcgcgt gtggaaatcg aagacctcgt
     4201 actggagcca ctagcgtcga gtgaggcggt gctgtcggat ggggagaccg atctcggtgt
     4261 ggcgctggtt gacatcggtg ggggtacgac tgacgtggcg gtctttaccg atggagcaat
     4321 ctggcactca gtggtgctgc caatcggggg gaatctgatt accagcgata tcgcgatcgg
     4381 cttgcgcctg ccattcgctg tagcagagga gctgaaggtg acctacggtc attgtgaccc
     4441 atcaactatc gccgatgacg acatgattga cctgtcgcac tacctgccag attgtaacga
     4501 tcttgtgcct cgaaagctcc ttgcagaaat cgtccaggcg cgtgtggaag agctatttga
     4561 gatggtacac gaggagatca agaaatccgg ttgtgacggg ttattgccag cggggctggt
     4621 agtaacagga ggtggcgctg aactgccagg gattctagac accgcggggc agattcttga
     4681 tttgccggcg cgtctggggt cgccacttgg tttg
//
LOCUS       DASZAK010000912         4205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_628633, whole genome shotgun sequence.
ACCESSION   DASZAK010000912 DASZAK010000000
VERSION     DASZAK010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4205
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628633"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="VGT44_15995"
     CDS             <1..305
                     /locus_tag="VGT44_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV2582358.1"
                     /translation="FAVQADGIAITTIEGMAGSQSDLHPIQQAFMEKHGLQCGFCTPG
                     IIMSVHALLHEHPSPTEQEIRHELSGNLCRCTGYQNIVEAVKLAAERLRAQQMEVE"
     gene            305..2692
                     /gene="cutA"
                     /locus_tag="VGT44_16000"
     CDS             305..2692
                     /gene="cutA"
                     /locus_tag="VGT44_16000"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF040766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEV2582359.1"
                     /translation="MATRWFGAPVQRQEDPRLLRGRGSYVDDIDLPTMLHAAILRSTH
                     AHARIVSIDASAARDLPGVHLVLTARDLGEKLEPSPLLVPHHALTQPRTQLPLALDEV
                     RYVGEAVALVVAEDRYIAEDAFDLIEVEYEPLPVVHSLAVAAADNAPLVHADVPGNLA
                     ARLVQTVGDPDSVFTQATHVVRESMVIDRGAAMPMECRGVLARWDAHEGMLTCWISTQ
                     GPIPIRNGLAAIFQLPEHKVRVIAPDVGGGFGTKIMMFYPEEVLVPLAAIQLGRPVKW
                     IEDRREHFISANQERSQEHEVEYAYDDQGILLAVRDRFLHDAGAYTPYGIIVPIITAC
                     TLPGPYRLRHYASEFSVLYTNKVPVSPYRGAGRPHAVFVMERIMDRIALELGLDRLEV
                     RSRNFIRPDEFPWDVGLIYQDGGPTKYDSGNYQAGLEKLKALLEYDDFPAMQSEARAQ
                     GRYLGIGVACYVEGTGIGPYEGAMVRVESDGRVYASTGVTTQGQAHYTTFAQIVADQL
                     GVNPTDVQVTTGDSQSFYWGVGTYASRAATIAGSAMHLAAVKVREKAKQVAADLFEAS
                     PDDIELADGKVFVREAPHRALTLGQVAISSNPLRYAYGENARKLMSMKLAGPRPGPAL
                     PEERGSPGLEASAFYSPPHGSFASGIHGAIIEVDPKTGDVTFVKYAAVHDCGRVINPV
                     VVEGQVHGGVAQGIGGAFFERLVYDEQAQLLNASFMDYLIPTAAEIPSLLVDHVETPS
                     PLNPLGVKGAGEAGVIPVPALFASAIDDALAPLHLRVLEMPLHPRRLFELLNERR"
     gene            complement(2800..3255)
                     /locus_tag="VGT44_16005"
     CDS             complement(2800..3255)
                     /locus_tag="VGT44_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582360.1"
                     /translation="MAEPSAPFVAPATVGQSETAFGVEGAATAGRNCPTKLVDSTLAP
                     APKLLVNRYCPIVFPTAPVAAIAPDAGLDSAEAMPGFVPGPIGVMQNTRRKDRRLSHS
                     AEVEFRSENNVEKVDYQPVDRPRTCITLHQIKKAPVKESNIITLSPKEC"
     gene            3234..4145
                     /locus_tag="VGT44_16010"
     CDS             3234..4145
                     /locus_tag="VGT44_16010"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycoside hydrolase"
                     /protein_id="HEV2582361.1"
                     /translation="MAQMAQPSQFQPVAGGFVNSASNGNGYYQGLQGAVGTIGAPGIS
                     NTYASYDYYQEQEERSRPRIGLIIMIGVVVLLLVGGGLTGLLYYLNQPGASTPTQNFV
                     ITTPTSHPLFRDSFANNTTGWLLSNNPGASSQQVGGGKMTLEDDNNQLLWEILPGKTF
                     SDFRLDVDATLTKGNTSNAYGIYIRGVSTATSDIGTYYRLEIYGDGTFAVFKGAQDSS
                     GNTVNQQVYYSKSPVSAILTEGHSNHITIIAKGSSMVFMVNGVTIYSYSDTTYRSGVV
                     ALFVSNLPNLSGGAQATFANLAIFPAS"
BASE COUNT          856 a         1283 c         1232 g          834 t
ORIGIN      
        1 tgttcgctgt gcaggccgat ggcatagcca tcaccaccat agaaggcatg gcgggaagtc
       61 agagcgacct gcatcccatc cagcaagcct tcatggagaa gcatggccta caatgtggct
      121 tctgcactcc cggcattatc atgtcggttc acgcgctgct gcacgaacac ccaagtccga
      181 ccgagcagga gatacgacac gaactctccg gcaatctctg tcgttgtacc ggctatcaga
      241 atatcgtcga ggcggtgaaa ctcgcggctg agcgcctgcg agcacagcaa atggaggtgg
      301 aataatggcc actcgctggt tcggcgcgcc ggtacagcgc caggaagacc cacgtttgct
      361 gcgggggcgc ggttcctatg tcgatgatat cgacctgccc accatgctgc acgccgccat
      421 tttgcgcagc acacacgccc atgcccgcat cgtctcaatc gacgcctccg ccgcgcgtga
      481 cctccccggt gtgcatctcg tgctgacagc gcgcgacctg ggtgagaagc tggaaccatc
      541 gccgttgctc gtaccacacc acgccctgac acagccgcgc acgcaacttc cgctcgcgct
      601 cgacgaggtg cgctacgtcg gcgaagcggt cgcactggtg gtggcggaag atcgctatat
      661 cgccgaagat gcgtttgact tgatcgaggt tgagtatgaa cccctgccgg tcgtgcatag
      721 cctggcggtc gcagcagccg acaacgctcc gctcgttcac gccgatgttc cgggcaatct
      781 tgccgcgcgc cttgtccaga ccgtcggcga ccccgacagc gtcttcaccc aggcgaccca
      841 tgtcgttcgt gagagcatgg tgattgatcg cggcgcggcc atgcctatgg aatgccgggg
      901 agtactggcg cgctgggatg cccatgaagg aatgctcacc tgctggattt ctacgcaggg
      961 gccgatcccc atccgcaacg gcctggcagc catcttccag ttgcccgaac acaaagtgcg
     1021 cgtgatcgcg cccgatgtcg gcggtggctt cggcactaaa atcatgatgt tctacccgga
     1081 agaggtactg gtgcctctcg cggccatcca gctggggagg cctgtcaagt ggatcgagga
     1141 tagacgcgaa cacttcatct ccgccaacca ggagcgcagc caggaacacg aggtggagta
     1201 cgcctacgac gaccagggta tcctgctggc tgtacgcgac cgattcctgc atgacgcggg
     1261 agcctatacg ccctacggca tcatcgtgcc gattatcacg gcctgtacat tacccggccc
     1321 ctatcgcctg cgtcactatg ccagcgaatt cagcgtgttg tacaccaaca aagtccccgt
     1381 cagcccctat cgcggcgcag gccgacctca cgctgttttc gtcatggaac gtatcatgga
     1441 ccgcatcgcg ctcgaactcg gcctggatcg cctggaggtg cgctcgcgta atttcatccg
     1501 ccccgacgag ttcccctggg atgttggcct gatatatcag gacggggggc cgacgaagta
     1561 tgacagcggc aattaccagg ccggtttaga aaagctgaag gcattgctcg agtatgatga
     1621 tttccccgcc atgcagtccg aggcgcgcgc gcaggggcgc tacctgggca tcggcgtcgc
     1681 ttgttacgta gaaggcaccg gcatcggccc ctacgagggc gcgatggtgc gcgtggagtc
     1741 ggatgggcgc gtctacgcct caacaggcgt caccacgcag gggcaggcac actatacgac
     1801 tttcgcgcag atcgttgccg accagctcgg cgtcaatccc accgatgtgc aggtcacaac
     1861 cggcgattcc cagtcgttct actggggcgt cggcacgtat gccagtcgcg ccgccactat
     1921 cgcgggttcg gccatgcacc tcgccgccgt caaggtgcgc gagaaagcga aacaggttgc
     1981 ggcagatctc ttcgaggcgt cgcccgacga catcgagctg gccgacggca aggttttcgt
     2041 gcgggaagcg ccccaccgcg ctttgacgct ggggcaggtc gccatctcct ccaacccgct
     2101 gcgttatgcc tacggcgaga acgcgcgtaa gctgatgtcg atgaagctgg ccgggccgcg
     2161 accagggcct gctctgccgg aggagcgcgg cagccccggc ctcgaggcca gcgcattcta
     2221 cagcccgccg cacggcagct tcgccagcgg catccacggc gcgatcatcg aagtcgatcc
     2281 caaaacgggt gatgttacct tcgtgaagta cgcggcggtg catgactgtg ggcgcgtgat
     2341 caacccggtg gtcgttgaag gacaggttca cggcggcgtc gcgcagggca tcggtggcgc
     2401 gttcttcgag cgcctggtct acgacgagca agcacaactg ctcaacgcta gctttatgga
     2461 ttacctgatc ccgacggcag ccgagatacc gtccctgctc gtcgatcatg tcgaaacgcc
     2521 ttcgccgctc aatccgctcg gcgtgaaggg cgcgggcgag gccggcgtga taccggtgcc
     2581 tgcactgttc gcctccgcca tcgacgatgc gcttgcgcct ctgcacctgc gagtgctgga
     2641 gatgccgctg catccgcgac ggttgtttga gctgctcaac gagcgccggt gagtcggcca
     2701 ctacccaaat atgctacccg caagacgaac aagcggtgtt atacgttact ccattaagtc
     2761 acacagaagc gcggggggaa cggggagaag agcgcatggt taacattcct ttggggatag
     2821 ggtaataatg ttgctctctt ttactggcgc ttttttgatt tgatggaggg taatgcatgt
     2881 cctcggtcta tccaccggct ggtagtcaac tttttcgacg ttgttctcgc tgcggaactc
     2941 cacttccgcc gaatgggaca atctgcggtc tttgcggcgc gtattctgca ttaccccaat
     3001 gggaccaggg acaaaaccag gcatggcctc agcagaatca agcccagcat caggcgcaat
     3061 ggcagccaca ggcgccgtcg ggaacactat ggggcaatag cggttcacca gcagcttcgg
     3121 ggcaggcgcc aacgtggagt caaccagctt tgtcgggcaa tttctcccag ccgttgcagc
     3181 cccctcaacc ccaaaggccg tttcagactg cccaaccgtc gcaggcgcca caaatggcgc
     3241 agatggctca gccatcgcaa tttcaaccgg tcgccggggg ttttgtcaat agcgcgagca
     3301 atgggaatgg atactatcag ggattacagg gggcggttgg aacaatcggc gcgcctggaa
     3361 tttcaaacac ctatgcatcc tacgactatt atcaggagca ggaagagaga agcagaccgc
     3421 gcatcggctt gatcattatg atcggcgtgg ttgtcctgct actcgtcgga ggtgggttga
     3481 ccgggctgct ctactacctc aatcaaccgg gcgcatcaac acctactcag aactttgtga
     3541 ttacgacgcc gacctctcat cccctcttcc gcgattcatt tgcgaacaac actactggct
     3601 ggctgctctc aaacaatccc ggtgcatcgt cccagcaagt aggcggcggc aagatgacgc
     3661 tcgaagatga caataaccag ctgctgtggg aaatattacc gggtaagacc ttctctgatt
     3721 tccgtctgga tgtggatgcc acgctgacaa agggcaatac gagcaacgcc tatggcatat
     3781 atatccgggg cgtatcgacg gccacgagtg acatcggcac ctactaccgg ctggaaatct
     3841 acggcgacgg cacgtttgct gtttttaagg gagcgcagga tagtagcggc aatacggtca
     3901 accagcaagt ttattactcg aagtcgcctg tttccgctat cttgaccgag gggcatagca
     3961 atcacatcac gatcattgcc aaagggagca gtatggtctt catggtcaat ggcgtaacca
     4021 tctacagcta tagcgatacg acgtatcgca gcggagtcgt ggccctgttc gtttccaatt
     4081 tgccgaattt gtcaggcggc gcgcaggcta cctttgcaaa cctggcaatt tttcctgcca
     4141 gctaacctct atcctgcatc agcgcctgcg cgtagaccca cggatcaacg ttgccgccac
     4201 tgacg
//
LOCUS       DASZAK010000913         2126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_555212, whole genome shotgun sequence.
ACCESSION   DASZAK010000913 DASZAK010000000
VERSION     DASZAK010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555212"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1775
                     /locus_tag="VGT44_16015"
     CDS             <1..1775
                     /locus_tag="VGT44_16015"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582362.1"
                     /translation="VLYYGAGEAQSASAENFIVLPIIFGLVTACFIPLSRAFGQLFTR
                     VKPLTAYTFDIIGSLLGIAIFTAMAFFVLPPLVWFGILTLLILLLSSKRTVLASAAAL
                     FASLVIVGQLQIGAYWSPYYKILLHPALPDGYIVDVNNAGGHQTMQSWQYKEPFYRLV
                     YELFPGSSFHHALILGAGSGSDVAVALHYGVQSITAVEIDPTIQQLGARYHPNHPYSD
                     PRVKVVINDGRAFLENTTEKYDLIIFALPDSLTLTSSNTSLRLESFLLTQDSIAKARS
                     RLTDNGIVVLYNYYREDWLVQKLATMAGHAFNNQEPLVETYGGWGRAAVIMDGPRLIT
                     LPPGQFGPYHQQASVGTNRLSVIGEGYYPLSSITPATDDWPFLYLRDRSFPTIYIAAL
                     AMVLLFAIIGSLALAPRGVLRRFDWHMFFLGVAFMLLEVKSLTTFSLLFGSTWLVNSL
                     VFFAILSSVLLAILVNSRLKVRRIWPYYLLLFGILAVNLLLPPETLLLGNAAARYLLA
                     SLLAFTPVFLANIIFANSFRDSETADIAFASNLLGIMIGGGLEYFSMLIGYRWLLLLV
                     MVFYACAMLLRNRHKTSAGVAQLS"
BASE COUNT          402 a          623 c          569 g          532 t
ORIGIN      
        1 aggtgctcta ttatggagcc ggggaagccc aaagcgccag cgcggagaat ttcatcgtcc
       61 tgcctattat attcgggctg gtgaccgcat gctttattcc cctttcccgc gcatttggcc
      121 aattattcac gcgggtgaag ccgcttaccg cgtatacatt tgatatcatc ggcagcctgc
      181 tagggattgc catcttcaca gccatggctt tctttgtcct tccacctctt gtctggttcg
      241 gaatcctgac actgctgatc ttgttgttga gtagcaaacg gacagtgctg gcgtcagccg
      301 cggcgctctt tgcgagccta gtcatcgtcg ggcagctcca gatcggagca tactggtcgc
      361 catactacaa gatcctcctt caccctgccc tcccggacgg ctatatcgtt gatgtgaaca
      421 acgcaggtgg tcaccagaca atgcaatcct ggcaatataa agaaccattc taccgcctcg
      481 tgtacgaact cttccctggc agttcattcc atcacgccct gatcctgggt gccgggagtg
      541 gctccgatgt cgccgtcgcc ctgcactacg gagtccagtc gatcacggcg gtggagattg
      601 atcctacgat ccagcagctt ggcgctcgct accatccaaa ccacccctac agtgaccctc
      661 gcgtgaaggt tgtcatcaat gatggccggg ctttcctgga gaacacgacc gagaagtacg
      721 acctgattat ctttgccttg cccgactcgc tgaccctcac ctcgagtaac acgagcctgc
      781 gtcttgagag ctttctgctc acacaggact cgatcgctaa ggcccgctcg aggctgactg
      841 ataacggtat agtggttctt tataactact accgtgagga ctggttagtg cagaaactcg
      901 ctaccatggc cgggcatgcc ttcaacaacc aggaaccact tgtcgagacc tatggcgggt
      961 ggggaagagc cgcggtcatc atggatgggc cgcgcctgat aacactcccc cctggtcagt
     1021 tcggtccgta tcaccagcaa gcttcggtcg gcacgaaccg gttgagcgtc attggcgagg
     1081 gctactatcc gctttcctca atcacaccgg cgaccgatga ttggcctttc ctgtacctgc
     1141 gcgatcggag cttcccaacg atctacatcg ctgctcttgc catggtcctc ctgttcgcca
     1201 ttatagggag cctggcactc gcgccccggg gagtcctgcg ccgcttcgat tggcatatgt
     1261 tcttcctggg cgtcgcgttc atgctgctcg aggtcaagag tctgaccacg ttctcgctct
     1321 tattcggcag tacctggctg gtcaactcac tggttttctt cgccatcctg agcagcgtat
     1381 tgttggctat cctggtgaac agccggctca aggtccgccg catctggccg tattacctgc
     1441 tgctgttcgg catactcgca gtcaatttac tcctaccgcc cgaaacgctt ctgctgggaa
     1501 acgcggctgc gcgctatctc ctcgcaagtt tactggcctt cacgccggtg ttcctggcca
     1561 acatcatttt cgcgaattcg tttcgggata gcgagaccgc ggatatcgct tttgcctcga
     1621 acctgctggg catcatgatt ggaggcggtc tggagtactt cagcatgctg atcggctatc
     1681 gctggctgct tctgctggta atggtgtttt atgcctgcgc catgctgctc cggaatcgtc
     1741 acaaaacgtc agccggggtc gctcaactat cgtaatgcca gacgatctat acatcctgcc
     1801 gggatactgc gcatggacgc cgtattcgct gttcgagtgt ttcttgacca ggaagcaact
     1861 cgtgctttga ggctctaagc tggagaagta ccttgtagcc agacatgaca gtgggcgctt
     1921 ttatcccctg tgtacgaact gtgtgagcag ggtagagacg gtgggtgggc tattacccag
     1981 catagaagtg tggcaaagca cacgcatggc ctccctggcc gttgtgcgag gagtcgattc
     2041 agagcaaagc tcaaacagtg ggttgttcgt ctgctcttcc tgccgtagcg gacggcgctt
     2101 ttacgccgtc gatgtagatg tggcgc
//
LOCUS       DASZAK010000914         4108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_165578, whole genome shotgun sequence.
ACCESSION   DASZAK010000914 DASZAK010000000
VERSION     DASZAK010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165578"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VGT44_16020"
     CDS             <1..993
                     /locus_tag="VGT44_16020"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV2582363.1"
                     /translation="IDQSHDTSYTDCMNNMAQQESPGISVIYQDHYLLVLNKTAGLVI
                     HPTYKHADGTMWDALLVELARQGSDGWQPPTLPDMPGWERAPARIREMLREQQRLKYI
                     QKEGWLPRPCLLHRLDKDTSGVVALARTERARRHLSRQFQQHTIVKRYLAVVGRSAPD
                     WAARQMKVNRHCEDGQIIEVDAAGLWETRDEFTLEGALRRDPDDRRRCIVDPAGQSAV
                     TVVKTLAVNDSYALLEARPVTGRTHQIRAHLAAAGFAIVGDATYGLTAGEGRPEDLLH
                     RQFLHANSLELHRYPDNVQCTYVAPLAAELAAWLTNYFPEGMEVVHATSGISAG"
     gene            complement(1899..2921)
                     /locus_tag="VGT44_16025"
     CDS             complement(1899..2921)
                     /locus_tag="VGT44_16025"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV2582364.1"
                     /translation="MMKINQLLLAARHPAAVPLPIEGELPSLGGATTWLNSPPLSAAD
                     LRGNVVLIDFWTYTCINWLRTLPYVRAWAEKYREHGVVVIGVHTPEFSFEHDLENVRR
                     AARDMRVSYPIAIDNDYAIWDAFENHYWPALYLVDAQGRLRAHHFGEGAYEQSEMMLQ
                     QVLAEADLGGLAPELVSVEAQGAEAAADWGDLRSPENYVGDARTENFSSPGGAVIDKP
                     RVYSVPARFSLNHWALSGEWTVEREATELNAANGRIAYRFHARDVHLVMGPAARGTSV
                     RFRVFLDGQAPGPAHGIDVDEQGNGTVTEPRLYQLIRQPKPIADRQFEIEFLNAGVQA
                     FAFTFG"
     gene            complement(3203..>4108)
                     /locus_tag="VGT44_16030"
     CDS             complement(3203..>4108)
                     /locus_tag="VGT44_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2582365.1"
                     /translation="DNALPLIVTHGWPGSVIEQIKIIEPLTNPTAYGASASDAFDLVI
                     PSLPGHGFSGKPTTTGWDPIRIARAWIVLMQRLGYTRSVAPGGDWGNAVTEQMALVAP
                     PALLGIHTNMPATIPDDVAKALQFGDPPPSSLSADERHAYDQLDFFYKHGVAYAQEMG
                     NRPQTLYAMADSPVGLAAWMLDHDAASYALIARVFDGQTEGLSRDDVLDNVTLYWVTN
                     TVVSSARLYWESKLAFFAPKGIAIPVAVSAFPDELYQAPRSWTERAYPKLIYYNKLDK
                     GGHFAAWEQPQLLSEEIRVGFRSLR"
BASE COUNT          823 a         1209 c         1259 g          817 t
ORIGIN      
        1 attgaccagt cccatgatac ctcctatact gattgtatga ataacatggc tcaacaagaa
       61 tcaccaggca tctccgtcat ttaccaggat cattatttgc tcgtcctcaa taaaactgcg
      121 gggctggtga tacatcccac atataaacat gccgatggga caatgtggga tgctttgctg
      181 gtggagctgg cacggcaggg aagcgatggt tggcagccgc ctacgttgcc ggatatgcca
      241 ggatgggaac gcgctcccgc gcggatacgc gagatgctgc gcgaacagca gcgactgaaa
      301 tatattcaaa aagaaggatg gctgccgcgc ccctgcctgc tgcaccgcct ggataaagat
      361 acgtctggcg ttgtagcgct ggcgcgcaca gaacgtgccc gccgccactt gagccgccaa
      421 ttccagcagc acaccatcgt caagcgctac ctggcagtcg ttggcaggag cgcgccggac
      481 tgggccgctc gtcaaatgaa agtgaatcgc cattgcgagg atggccagat aatcgaggtt
      541 gatgccgctg gattgtggga aacgagagat gagttcacgc ttgaaggtgc cttgcggcgc
      601 gacccggacg atcgccgccg ctgcatcgtt gacccggcag gtcaaagcgc tgtcacggtg
      661 gtgaagacgc tggcagtgaa tgattcctat gctctgctag aggcgcggcc tgtgacgggg
      721 cgcacgcacc agatacgggc gcatctcgcg gcggctggct ttgccatcgt gggggatgcg
      781 acctacgggc taactgccgg ggaaggaaga ccggaagatt tgctgcatcg ccaatttctc
      841 catgccaata gcctggaatt gcaccgctac ccagataatg tacaatgtac ctatgtcgct
      901 ccattggctg ctgaactggc agcatggctg acaaactatt ttcccgaagg gatggaagta
      961 gttcatgcaa caagcgggat atccgccgga tagcgggcct tacgcgcgcc tgaacagcga
     1021 ggagaagaaa agatggcact tttgaaagga tagcagacga tggaggcacg gtttctttgc
     1081 gtattttgct cgtcggacgc cgactcttct gcctgacccc atgcactcct tcaccgccac
     1141 gctctcgcgc ctactgcccg accttgcact gctgctccct gacctggcgg ccttgcccac
     1201 agctccctct gttgatccag aagaggagaa gcgacggctc tgacctgagc ctggacttgc
     1261 tgctgcacct ggcccgccgc tgccgacagg caccgctcct cttgctcgtc acctatcgca
     1321 gtgaggaact gcatccacgc ttgaggcagt caagatcgag acacggtgct cgccaaacca
     1381 caaattcaat caggtttctg ctcgtttgat ggtgggctgt ctcactttac ttgcacacgt
     1441 tcctccatgg ggctgacacc ctggtctcct gtcaagaaat cttcggctgg gagcctggct
     1501 gattcggcgg aaggcaagtt cggtaattcc gttgctgaac gtggtggtcg agacgatctc
     1561 gagcctgagt gattgttcca ggtcgctgaa aagccgcgcg cccttgcctg ccaggtaggc
     1621 ctaatacata cattggtgac accagaggca agatagctat atagctggag aggaacgacg
     1681 cgtacccagt tttgtgccca ttgcaccatg tccgccaggc tcgtcagtct caccggtagc
     1741 tcttgttgct cggcggtgta ttcttgcagg tctcgaagcc ctcgcaatga ggactgcctt
     1801 gcagcgcgtg ccaggaaccg agccattccc gctgcgaggc ttttcgttgc tcctggtatc
     1861 acacctgcct ctcctcaagt tccctccgct gtcgtagatc agccgaaggt gaacgcgaaa
     1921 gcctgcacgc ctgcattgag aaactcaatc tcgaactgac gatcagcaat aggcttcggt
     1981 tgccggatca gttgatacag ccgcggttcg gtcaccgtgc cgttgccctg ctcgtcaacg
     2041 tcaatgccat gagccgggcc cggagcctgt ccatcgagga acacgcgaaa gcgtacggaa
     2101 gttcctcgtg ctgccggccc catgacgaga tgtacatcgc gggcatgaaa gcgatacgcg
     2161 atgcgcccgt tggccgcgtt cagctccgtg gcctctctct ccactgtcca ctcacccgaa
     2221 agggcccaat gattgagcga gaagcgcgcg gggacagagt agacgcgagg cttgtcaatg
     2281 actgccccac caggagacga aaagttctcg gtgcgcgcgt ccccgacata gttttctgga
     2341 gaccggaggt ccccccaatc ggcggcagct tcagccccct gggcctcgac cgagaccagt
     2401 tcaggagcga ggccgccgag gtcggcctcg gccagcacct gttgcagcat catttccgac
     2461 tgttcgtagg cgccctcgcc gaagtgatga gcgcgaagac gtccctgcgc atcgacgaga
     2521 tagagggctg gccaatagtg gttctcgaac gcgtcccaga tcgcatagtc gttatcgatg
     2581 gcgatcggat aggagacccg catgtcccta gcggcccggc gcacgttctc caggtcgtgc
     2641 tcaaaggaga actccggcgt gtgcacgccg atcaccacca cgccgtgctc ccggtatttc
     2701 tcggcccagg cccgcacata gggcagggtg cgcagccaat tgatgcaggt gtaggtccag
     2761 aagtcgatga gcacgacgtt cccgcgcagg tcggccgccg agaggggcgg cgaattgagc
     2821 caggtggtcg cgccacccaa agaaggcaac tccccttcaa tgggtagggg aaccgccgcg
     2881 gggtgtctcg cggcgagcaa gagctggttt attttcatca tgaatgcctt tcctctgctt
     2941 ggatgtctca gggatgcgcc tctgcaccat gcgggacgcc aacatcgagg tgcatacgtc
     3001 tcgccatgta gcttgggaag cgaccagcta gttaagagac ccggcagtgc ggggggttgt
     3061 ctgagacatg agcattcctt tctcagatgg gtgtcaacca accagtcaat gagatcggtg
     3121 acagatcggc caatgagtac atcgtttctg ctgcgggggc cgggacgacg gacacacgtt
     3181 gtgcccgcca tcctctctcc ctctagcgca gggacctgaa gccgacgcga atctcttctg
     3241 aaaggagttg cggctgttcc caggccgcaa agtgcccgcc tttgtcgagt ttgttgtaat
     3301 agatgagttt gggataggcg cgctcggtcc aactgcgcgg ggcctggtag agctcgtcag
     3361 gaaaggcgct cacagccacc gggatcgcga tccctttagg agcgaaaaag gccagcttgc
     3421 tttcccaata caggcgagcc gaggagacca ccgtgttcgt cacccagtag agcgtcacat
     3481 tgtcgaggac gtcgtctcgg ctgaggcctt cagtctgacc gtcaaagacg cgggcgatga
     3541 gcgcgtaact ggccgcgtca tggtcgagca tccaggccgc caggccgacg ggtgaatccg
     3601 ccatcgcgta gagcgtctgg gggcggttgc ccatctcctg ggcgtaggcc acgccgtgct
     3661 tgtagaaaaa gtccagctgg tcgtaggcgt gccgttcgtc ggctgagaga ctggaaggcg
     3721 gcgggtcgcc gaactgcagt gccttggcca cgtcgtctgg gatggtggca ggcatgttgg
     3781 tgtggatgcc gagcaatgcg ggaggcgcaa cgagagccat ctgctccgtc accgcattcc
     3841 cccagtcgcc gcctggcgcc acagagcgcg tgtagccgag ccgctgcatc agcacgatcc
     3901 acgcccgcgc gatgcggatg gggtcccagc cggtcgtggt cggtttgccg gagaacccgt
     3961 ggcccggcag cgacgggatc accaggtcga aggcgtccga cgcactcgcg ccataggcgg
     4021 tgggattggt caggggctcg atgatcttga tctgctcgat gaccgagccg ggccagccgt
     4081 gcgtgacgat gagcggcaag gcattgtc
//
LOCUS       DASZAK010000915         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_23611, whole genome shotgun sequence.
ACCESSION   DASZAK010000915 DASZAK010000000
VERSION     DASZAK010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23611"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VGT44_16035"
     CDS             complement(<1..188)
                     /locus_tag="VGT44_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582366.1"
                     /translation="MKRGYSILLLASMALALFVAACGSPSTPGTGGTSNQPETVTIWH
                     GWAGSYLAPKQAIFNAYM"
     gene            complement(439..954)
                     /locus_tag="VGT44_16040"
     CDS             complement(439..954)
                     /locus_tag="VGT44_16040"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2582367.1"
                     /translation="MFTEMDYLRLEIYRLISKSFIELETTDRQLMNQFNLSVTQYWAL
                     VHLEDPEGLPLSELAFLLIRDRSNMTSLVDKLEKEGLAERKYGKNGDRRFTRVALTEK
                     GHQLRSRVIVSHNYLINRRLETLSEESLRELRARLLELSDKLHSQLKNDEISSVVEDA
                     VQLRLSQTEIS"
     gene            complement(1063..1377)
                     /locus_tag="VGT44_16045"
     CDS             complement(1063..1377)
                     /locus_tag="VGT44_16045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582368.1"
                     /translation="MSRVHEKRRIRRPPSFTGLVIGSIPGLLILLLLVGNLIALPAWV
                     INVFDFIALALGLVAFVMSFILLRKEEHEHHGLGLLLTSVVSVLLAVYVLAWSLTGGN
                     FY"
     gene            complement(1487..2386)
                     /locus_tag="VGT44_16050"
     CDS             complement(1487..2386)
                     /locus_tag="VGT44_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582369.1"
                     /translation="MALTRKSLTEQGSNQSPPTVRRIWRTPGSLYNWLFLALSVIVLG
                     WVYLFYRLAIATQSYPGPYNEPFRAFGIVSFGLVIVVGAYTLRRRFVRSLPGKVQNWL
                     WLHIWLGVISILIAFMHDNFQNVTHDFMFNAMRFTEYGYGTLAMYALFVLVLTGVVGR
                     LLDVWQARTIAAEADTNGVGISRSLEDRLFELSLAVGRLRAGKSEQFKQYCEEALTGG
                     RSGFLPARVPMLAPQEVEDFQHTHELLSEYVRLGRSLQRQNRARLILKAWRYVHITLA
                     TLAFFVIAYHSVYELYKMIVLHY"
BASE COUNT          612 a          669 c          645 g          541 t
ORIGIN      
        1 ttcatgtagg cgttgaagat ggcctgcttc ggcgcaagat agctgcctgc ccagccatgc
       61 cagatcgtaa cggtttctgg ctggttcgat gtgccgccgg tgccgggtgt gctgggcgat
      121 ccacaagccg cgacgaacaa cgccagcgcc atactggcga ggagtaggat ggaataaccc
      181 cttttcatga gatcctccgc tttgagttgt cgaaaatacg tccatgttga tccgttgagc
      241 aacggatagc tgtacgaaga ctcctcctgc atgacagtaa aatccctttt gcaagaagaa
      301 tggtagaggg ttgaaaagtg tctcaggtag ctataatcac ctccttcaat tccattggaa
      361 gcaggtaaca tctacaaacg atcgtctcct acggcacaat agacaggcaa actataacag
      421 agttcctatg aggttttgct aactgatctc cgtctgtgac aggcgcaact gaaccgcatc
      481 ttcgacaacg ctggaaatct cgtcattctt gagctgagaa tggagcttat cgctcaactc
      541 taatagccgg gcgcgcaact cacgcaggct ctcttcagaa agggtctcca aacgccggtt
      601 gatcaaataa ttgtgcgaca cgatcacccg acttcgcaac tgatgccctt tttcggtgag
      661 agctactcgc gtgaagcggc ggtctccgtt cttgccgtat ttgcgttccg ccagaccttc
      721 tttttccagc ttatcaacca ggctggtcat attgcttctg tcgcgaatca aaaggaatgc
      781 cagctcgctt aacggaaggc cctctggatc ttccaggtgc acaagcgccc agtactgggt
      841 aacactgaga ttgaactgat tcatcagttg ccggtcagta gtttccagtt caatgaacga
      901 cttgctgata agccggtaaa tctctagcct gaggtagtcc atttcggtga acatcctaca
      961 gtttcctgtt agatagttga ctactcaact gatgctaaac atactatacc cggtaaatgg
     1021 gtgtttgtca atggttgaga gacggattat ctagatggtt cgctagtaaa aattcccgcc
     1081 cgtgagtgac caggccagca cgtacaccgc cagcagcact gaaaccaccg aggtgagcaa
     1141 cagccccaga ccgtgatgct cgtgttcctc tttgcgcagc aggatgaaac tcattacgaa
     1201 cgccacgagg ccaagcgcca gcgctatgaa gtcaaagaca ttgatgaccc acgcgggcag
     1261 cgctatcaga ttgccaacca ggagcagcag tattagcagg ccggggatac tgccaatgac
     1321 caggccagtg aaggacggtg ggcggcggat gcgccgcttt tcatgaactc ttgacatcaa
     1381 tgatctcctc taatccacta aatcctctta gatacagcta ttttatctta ttcgttgatc
     1441 agtttctaga ggggatgatt tacaggagcg gaggagatga aaggtatcaa taatgcagca
     1501 caatcatctt gtacagttca tagacgctat gataggcaat cacgaaaaaa gccagggttg
     1561 ccagcgtaat atgaacatat ctccatgcct tcaggatcag tcgcgcgcgg ttctggcgtt
     1621 gcagcgaccg accaagccgg acatactcgc tcagcagttc gtgcgtgtgc tggaaatcct
     1681 caacctcttg aggagcgagc atcggtacgc gggccggcag gaagccgctt ctgccgccgg
     1741 tcagcgcctc ttcgcaatat tgcttgaact gttcggattt tccggcgcgc aggcgaccca
     1801 ccgccagcga tagctcgaag aggcgatcct ccagcgagcg cgagatgccg acgccgttgg
     1861 tatccgcctc cgccgcgatg gtgcgcgcct gccacacatc cagcaggcgg cccacaacgc
     1921 ccgtcaagac cagcacgaac agcgcgtaca tggccagtgt gccatagcca tactcggtga
     1981 atcgcatagc attaaacatg aaatcgtgtg tgacattctg gaaattatcg tgcatgaagg
     2041 cgatcaatat gctgatcacg ccgagccaga tatgcagcca gagccagttt tgcaccttcc
     2101 ccggcaggct gcgcacaaag cgccgccgca gggtatacgc cccgacgacg atcaccagcc
     2161 cgaacgagac gatgccaaag gcgcgaaacg gttcgttata ggggccagga taggactgcg
     2221 tggcgattgc cagccggtaa aacaggtaga cccagccaag cacgatgacc gacagcgcca
     2281 gaaagagcca gttatagagc gaccctggcg ttctccagat gcgccgcacc gttggcggtg
     2341 attgatttga gccttgttca gtcaacgatt tgcgtgtcag tgccatgagt caaaataccc
     2401 ttatccagat gagtcacgat cccaggtgag ttccgagggc gaccgcaagg gtacgcccct
     2461 acaatga
//
LOCUS       DASZAK010000916         1916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_663361, whole genome shotgun sequence.
ACCESSION   DASZAK010000916 DASZAK010000000
VERSION     DASZAK010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663361"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..>1916
                     /locus_tag="VGT44_16055"
     CDS             135..>1916
                     /locus_tag="VGT44_16055"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF003253.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HEV2582370.1"
                     /translation="MHIPDYVSHAKLRAWVEAMVALCQSDHVHWCDGSQEEYDSLCEQ
                     MVESGTFIRLNSEKRPNSFLARSDPSDVARVEDRTFICSLSKNGAGPTNNWVHPRQMK
                     ETLHGLFNGCMRGRTMYVIPFSMGPLGSPIAHIGVQLTDSPYVAVNMRIMTRMGRAVY
                     DVLSQQGEFIPCMHSVGMPLEPGQRDVAWPCNQEHKYIVHFPEERSIWSFGSGYGGNA
                     LLGKKCFALRIASVLARDEGWLAEHMLILGVETPHGEKSYVAAAFPSACGKTNFAMLI
                     PPPAFQEEGWKVTTVGDDIAWIKPGPDGRLYAINPETGFFGVAPGTSEKSNPNAMASM
                     RANSIFTNVALTEDGDVWWEGMTKTPPARLVDWTGQDWTPADKRPAAHPNARFTAPIS
                     QCPVIDVAADDPRGVPIAAFIFGGRRSNTVPLVYQAFNWNYGVYAAATMGSETTAAAT
                     GKVGEVRRDPFAMLPFTGYHMASYFNHWLDFGRHLPNPPRIFCVNWFRRDEDGNFLWP
                     GFGENMRILKWIIERAHGRSTSVECPIGWVPRYEEIDWRGMDDFTRQQFADLMTVNRD
                     LWQKELLAHEELFIKLYDRLPKELLFIR"
BASE COUNT          385 a          580 c          566 g          385 t
ORIGIN      
        1 gggtgtcggc tggtgtataa taaatgatta gggtctctat ggctttatgc agatgcttgt
       61 gtacaagtgt ccgcgtttat tttgaacagt gcagtaatca cctcatttct ctctattttg
      121 aaaggcagga atcgatgcat attccagatt acgtctctca cgcaaagttg cgcgcctggg
      181 tcgaagcgat ggtcgcgttg tgccaatccg accatgttca ctggtgcgac ggatcacagg
      241 aggaatatga tagcctctgc gagcagatgg tcgagtcggg tactttcatc cggctcaaca
      301 gcgagaaacg ccctaacagc ttcctggcgc gctccgaccc cagcgatgtc gcgcgcgtcg
      361 aggatcgcac gtttatttgt tcgttgagca aaaacggtgc cgggccgacc aacaactggg
      421 tgcatccccg gcagatgaaa gagacgctgc acgggttatt caacggctgt atgcgcgggc
      481 gcacaatgta tgtcatccca ttcagcatgg ggccgctggg ttcgccgatc gcgcatattg
      541 gcgtccagct gaccgactca ccttacgtcg cggtcaatat gcgcatcatg acgcgcatgg
      601 gccgcgccgt gtacgacgtg ctgagccagc aaggcgaatt cattccctgc atgcactcgg
      661 tcgggatgcc attggagccg ggacagcgtg atgtggcgtg gccgtgcaac caggaacaca
      721 agtacattgt gcatttcccg gaggagcgtt caatctggtc gttcgggtct ggctacggcg
      781 gcaacgccct gctgggcaag aagtgcttcg cgctgcgcat cgcctcggta ctggcacgcg
      841 acgagggctg gctggcggaa cacatgctga tcctgggcgt cgagactccg catggcgaga
      901 agagctatgt cgccgccgcc ttccccagcg catgcggcaa gaccaacttc gccatgctca
      961 tccctccgcc cgccttccag gaagagggct ggaaggtcac aaccgtcggc gacgacatcg
     1021 cctggatcaa gccggggcca gatggcaggc tctacgccat caatcccgaa accggcttct
     1081 tcggagtcgc gcctggcacc agcgaaaaat cgaatccgaa cgcgatggcc tcgatgcgcg
     1141 ctaactctat cttcaccaac gtggcgctga ccgaagatgg cgatgtgtgg tgggaaggta
     1201 tgacgaagac gccgcccgcg cgcctggttg attggaccgg ccaggactgg acgcccgccg
     1261 acaaacgccc tgccgcgcat cccaatgcgc gtttcaccgc gccgatcagc cagtgtcctg
     1321 taatcgacgt tgccgccgac gacccgcgcg gcgtgccgat tgcggccttt attttcggcg
     1381 gcaggcgctc gaacaccgtg ccactggtct accaggcgtt caactggaac tacggcgtct
     1441 atgcggcggc cacgatgggt tcggagacaa cggcagccgc caccggcaag gtcggcgagg
     1501 tgcgccgcga ccccttcgcc atgctgccct tcacgggcta ccacatggcc agctacttca
     1561 accactggct ggatttcgga cgccacttgc ccaacccacc gcgcatcttc tgcgtaaact
     1621 ggtttcggcg cgacgaggac ggcaatttcc tctggcctgg tttcggcgaa aacatgcgca
     1681 tcctgaagtg gatcatcgag cgcgcgcatg gtcgatccac cagcgttgaa tgcccgatcg
     1741 gctgggtgcc gcgttacgaa gaaatcgact ggcgcggtat ggatgacttc actcgccagc
     1801 aattcgccga tctcatgacg gtcaaccgcg acctctggca gaaagaacta ctcgcgcacg
     1861 aagaactgtt tattaagctc tacgaccgtc tgccgaagga actgctgttc atccgc
//
LOCUS       DASZAK010000917         8328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_57972, whole genome shotgun sequence.
ACCESSION   DASZAK010000917 DASZAK010000000
VERSION     DASZAK010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8328
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57972"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="VGT44_16060"
     CDS             <1..551
                     /locus_tag="VGT44_16060"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582371.1"
                     /translation="VDLGAGSYERPGLALAGVTFKGRQERQLLTQTFAQERRSNELIV
                     EVIVPRLGYGYGASLQRVGRTPTDLALLNAAAVVEVTEGIYSHARLALGGVHMEPVRL
                     PGVERQLEGQSAMNADSSTINTQLLVSALQAGMADFQPPQEMRASASYRRSTAINLAF
                     RALEEAVTISRWRRMVSSKERQ"
     gene            555..3311
                     /locus_tag="VGT44_16065"
     CDS             555..3311
                     /locus_tag="VGT44_16065"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV2582372.1"
                     /translation="MEVEFRINGVIKSLDVAANESLLAALRREGYVSVKHGCETGECG
                     ACTVLIDGIPRPACVTFAAQVGGCTLATVESLGTAGALHPVQQAFVECGAIGCGFCAP
                     GMLLSAVALLKNNSNPSEEETRDALSGNLCRCTGYLKPVEAALRAAAVMRGEAVEPLE
                     TGQDIDAVKLVTGKAAFVDDIELRGMLHARLLTSPHAHAVIRDIDISAARAMPGVHAV
                     LTYRDLPRVPYASAGSFLSDAGPRDQYSLDYIVRYAGDRVAAVVADTPEIAEQALRLI
                     DVTYEVLPVVFDPRKAIDPAAPRLHPETESAGIYDAAHNIAAHFQAEVGDVEAAFASA
                     DQVIEGEYIVPQAQKASIETHLAITYLDEDDRLVVRSGAASAHAVQHVLGALINLPAR
                     RIRVVTPRIGGSAQPGILTEDICALLTLATHRPVRLEYARADEFRSSQSRPIQLMRLK
                     TGVRRDGSIVANAMTLLVGSGAYGSQTPHLLRDEGLNALPLYSCPNMRYTADAAYTNL
                     PPAGAYRGGASPAVFALECHMDEIAKQVGLDPLELRRRNQVNAGAENLLAKAWHQDIG
                     EHDGDNIYIIANNGLSQCLQIVEEKLAWKERRGKSAAGRFRTGIGVALAMHSTTASGA
                     SNKCGASIKLNEDGSFTLLVGMARTGDGTETIIAQLAAETLGVRLEDIILPATDTDTT
                     PFENGTGASDAICASAAVKAAQQAREHLLEAAGRLLNVEARSLTLEDGVITALNGDTA
                     TVAQVASYILSTAREMPIIAVAVEAGGRPSPSFAAHGAEVEVDTETGAVRVVRVITAL
                     DAGRVMNAHIAERQVEGGISRALGQAISEEMLYDQHGAPFTLNLSDYRIYCAPDMPQL
                     ETYFVDPGDSPGSNGLTSYAIAETSGLIPAIANAVADALGVRIKQLPLTPERVMRAIR
                     TQGA"
     gene            3348..4376
                     /locus_tag="VGT44_16070"
     CDS             3348..4376
                     /locus_tag="VGT44_16070"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEV2582373.1"
                     /translation="MTPINLHDYEKLAQKRIASHLWDFWQGGSDDEVTLRACRVAFER
                     IRLRPRMLVDVSVIDTRANVLGEQVAMPVLIAPTAAHCIGHPDGECATAQAAGRAGTL
                     MIASTVATRSLEEIAQAASGPLWFQLYAFRSREFTEQLVRRAEAAGYRAIVLTVDLPK
                     PGNRERDRRNNVTIPPEPYQEANFIGLTRGDLPLVSLTWDSIAWLRSVTTLPLLIKGI
                     LTAEDARLAVEHGVDGIIVSNHGGRQLDSAIPSIEALPEVVEAVNGRCEVYVDGGIRR
                     GTDILKALALGARAVLIGRPILWGLAVNGADGVTHVLELLRHELELSMALAGRPTLAS
                     IDRSLVSM"
     gene            complement(4414..7290)
                     /gene="mutS"
                     /locus_tag="VGT44_16075"
     CDS             complement(4414..7290)
                     /gene="mutS"
                     /locus_tag="VGT44_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV2582374.1"
                     /translation="MLFEDYLKENPVHSPIRTQYLQIKRQNPDAVLFFRMGDFYEMFD
                     DDAEIVARELEIALTRRDWGRGEKSPMAGVPHHAIDGYIARLVAKGYRVAVCEQTSDP
                     ALSKGLVEREVLRIVTPGTVIDPAMLAARRNNFLASVVVGREAVGIAYVDITTGEFAV
                     TQFSTPEPELALQQELGRIAPAEALVESRHSHQGSRKKRWLAAVMYEKTVTRVGSNGN
                     ANADIDETMAAAHETYNPSERGNGHSNGNALTPIQPDEEDDDDTAPMVRLLAGVAGHV
                     TPYDARYFAEDDARHRLLTHFEVASLEGFGCAHLPLAIRAAGAALAYVQETQKGLLRQ
                     LTSLETYFTNEYMTLDPHTRRNLELFESGRSGTVKGSLLWVLDKTRTPMGGRLLRRWI
                     GQPLLDIGILRHRQQMIAELLGDTLAQARLAEGLKRAGDIERLANRVRQRIATPRDLV
                     ALASGLRAAAEVRNGLRDTFAGENEEQLPALAQVARRLADNDETIALIERAIVDEPPL
                     NTSEGGVMRPGYSEELDTVKEASQHGQSWIAEMEQRERRRTGINNLRVGYTKVFGYFI
                     EVTNSNLGRVPQDYIRKQTLTNGERYITPDLKEHETLVLNAQERSSKLESELFAQLRA
                     DIAINASERILDTAHALAEIDVYLSLANVAAQHNYCRPQLNDGDTVHIVAGRHPVIEQ
                     SQTETPFIPNDVELSNHAAQILIITGPNMAGKSTYLRQVALIVLMAQIGSYVPAEAAT
                     IGIVDRIFTRIGAQDDLATGQSTFMVEMVETANILHHATPRSLVILDEIGRGTSTYDG
                     LSIARAVIEYLHNNKRCGARTLFATHYHELVEVAKSLPRIQCLNVAVTEEEGKVVFLH
                     KIVPGGADKSYGVHVAQLAGIPKPVIHRAEEILAELEQKGDAKARRKAMHDMLPATST
                     QMTLFSSEPHPLIEEIKQLAIDELTPIEAIGKLYDIQQKAKGRK"
     gene            7487..7912
                     /locus_tag="VGT44_16080"
     CDS             7487..7912
                     /locus_tag="VGT44_16080"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEV2582375.1"
                     /translation="MLLLILIIFALAGGTLAALAYENISTLSIDVRLTIFGWHSPAVA
                     LGVLILLSCVLGALLLYVVTLLSAVRERREMRRLRRRLAELEAMQAMMAEQRGQQGQM
                     DVSHQSQQPQTAHQMIVPMPGTQTYRLQYLFPPPPPKRE"
     gene            complement(7933..>8328)
                     /locus_tag="VGT44_16085"
     CDS             complement(7933..>8328)
                     /locus_tag="VGT44_16085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582376.1"
                     /translation="EVSEDTVEAEALLLQKLSTEADETDESNESNEDNQDDGSDTTLK
                     QAAVVLRANRITRPLPPDMPEQPDTPADTTEDMSEQPETPPDTPENTPDKPCQIEDDA
                     RYSEVPLVTLASAKKHGTMRRLVGRLLRG"
BASE COUNT         1536 a         2546 c         2557 g         1689 t
ORIGIN      
        1 aggtcgatct cggcgcgggc agctatgaac gccccggtct tgcgctggca ggtgtgacgt
       61 tcaagggcag gcaggagcgg caactgttaa cacaaacctt tgcgcaggag cggcgttcca
      121 acgaactgat cgtcgaggtg atcgtccctc gcctggggta tggctacggc gcgtccctcc
      181 agcgcgttgg gcgcacaccg accgatctcg cgctgctgaa tgccgcagcc gtagtcgaag
      241 tgacggaagg catctattcc catgcgcgac tggcgttggg tggcgtccac atggagccgg
      301 tgcgcctgcc aggagtcgag cggcaacttg aaggccagtc cgctatgaat gccgatagca
      361 gcaccatcaa tacccagctg cttgtctcag cgctacaggc tggcatggcc gacttccagc
      421 cacctcagga gatgcgcgcc agcgccagct accggcgcag cacggcgatc aacctggcct
      481 ttcgcgcgct ggaagaggcc gtcactattt cgcgctggcg tcgtatggtt tcgtcgaaag
      541 aaagacaata gttgatggaa gtcgaatttc gcattaatgg cgtcattaaa agcctggatg
      601 tggccgccaa cgaatcgctc ctcgccgcgc tgcgcaggga gggatatgtg agcgtcaaac
      661 atggctgcga gacgggcgag tgcggcgcgt gtacggtgct gatcgatggc ataccacgcc
      721 cggcctgtgt cacctttgcc gcccaggtgg gaggctgtac gctggcgacc gtagagagcc
      781 tcggaactgc gggcgcgctg cacccggtcc agcaagcctt tgtcgagtgc ggtgcgatcg
      841 ggtgcggatt ctgcgcgccc gggatgctcc tttcggcagt tgctctcctc aaaaataact
      901 ccaatccaag cgaagaagag acgcgcgatg cgctctccgg caacctctgc cgctgcaccg
      961 gctacctcaa gccggtagag gctgcgctgc gcgccgccgc agtgatgcgc ggagaagcgg
     1021 ttgagccgct ggaaaccggg caggacatcg atgccgtcaa gctggtcacc ggcaaagcgg
     1081 ccttcgtcga tgatatcgaa ctgcgtggga tgctccacgc gcgcctgctc accagcccac
     1141 acgcccatgc cgtcattcgc gacatcgaca tctccgcggc gcgcgcgatg cccggcgtcc
     1201 acgccgtgct gacctatcgc gaccttcccc gcgttcccta cgccagcgct ggatcattcc
     1261 tgtccgatgc cgggccgcgc gaccagtaca gcctggatta tatcgtgcgc tacgccggcg
     1321 accgcgtggc tgctgtcgtt gccgatacgc cggaaatcgc ggaacaggcc ctgcgcctca
     1381 tcgacgtgac gtatgaagtc ctgcctgttg tgttcgaccc gcgcaaggcc atcgatcccg
     1441 ccgctcctcg tctgcatcct gaaacggagt ccgccggtat ctacgacgcc gcgcataata
     1501 ttgccgccca cttccaggcc gaggtgggcg atgtcgaagc agctttcgcc agcgccgacc
     1561 aggtgatcga gggtgaatat atcgtgccac aagcgcaaaa ggcgtccatt gaaactcacc
     1621 ttgccatcac ctacctggac gaggacgacc ggctggttgt acgcagcggc gcggcctcgg
     1681 cccacgcagt acagcacgtc ctgggcgcgt tgatcaacct gcccgcgcgt cgcattcgcg
     1741 tcgtcacccc gcgtataggc ggcagcgcgc agccgggcat cctcaccgaa gatatctgcg
     1801 ccttgctgac gcttgctacg caccggcccg tccggctgga atacgcccgc gccgatgagt
     1861 ttcgctcaag ccaatcacgc ccgatccaat tgatgcgcct gaagaccggc gtcaggcgcg
     1921 acggctcaat tgtggccaat gccatgacgc tgctggtcgg ctcgggcgcg tatggctcgc
     1981 agacgccgca tctcttgcga gacgaagggc tgaacgcgct gccgctctac tcctgcccca
     2041 atatgcgcta cacagccgat gcggcctata ccaacctgcc acctgccgga gcatatcgcg
     2101 gaggcgcttc cccggctgtc tttgccctcg aatgccatat ggacgaaata gcgaagcagg
     2161 tcggactgga ccccctggag ttgcgccgcc gcaaccaggt caatgcgggc gcggaaaatc
     2221 tgctggcgaa ggcatggcat caagatatag gtgagcatga tggggacaat atctacatca
     2281 tcgcgaacaa cggattatcg caatgcctgc aaatagtcga agaaaaactg gcctggaagg
     2341 agaggcgcgg caaaagcgct gccgggcgat ttcgcaccgg aatcggcgtc gcgctggcga
     2401 tgcacagcac aacagcatcc ggcgcgagca acaaatgcgg cgcgagcatc aagctgaacg
     2461 aagatggctc gtttacgctg cttgtcggca tggccaggac gggcgatggc accgaaacca
     2521 tcatcgcgca gctggcagcc gagacgctgg gtgtgcgcct ggaagatatc attctacctg
     2581 caaccgatac cgatactaca ccgttcgaga acggcacagg agcttccgac gcaatctgtg
     2641 cgagcgcggc ggtcaaagct gcgcagcagg cgcgcgagca cctactcgag gccgccggtc
     2701 gcctgctcaa cgtcgaagcg agatcgctga ctctggaaga cggcgttatc actgcgctca
     2761 atggagatac tgccacggtt gcgcaggtcg cgtcctatat cctctccact gcgcgggaaa
     2821 tgcccatcat cgcagtcgcg gttgaggcag gcggcaggcc atcgccctcc ttcgcagcgc
     2881 atggggcgga agttgaggtg gataccgaga caggcgccgt gcgcgtcgtg cgcgtcatta
     2941 ccgcccttga cgccgggcgc gttatgaacg cacatatcgc agagcgacag gtcgaaggcg
     3001 gtatcagtcg tgcgctcggc caggccatca gcgaagagat gctctacgac cagcacggcg
     3061 cgcccttcac tctcaacttg agcgattacc gtatctattg cgcgcccgat atgccgcaat
     3121 tggaaactta ttttgttgat ccaggcgatt cacccggctc aaacgggcta acttcatatg
     3181 ctatcgcaga aacgagcgga ctgatccctg ccattgccaa cgccgtcgcg gatgcgctgg
     3241 gtgtgcgcat caaacaactt ccactgacac cggagcgggt gatgcgagct atacggacgc
     3301 agggggcgta ataagagcaa tcaaaaactt agaaagggaa tccatttatg acccccatca
     3361 acctccacga ctacgaaaaa ctcgcccaga agcgtatcgc ctctcacctg tgggacttct
     3421 ggcagggggg cagcgacgac gaggtgacgc tgcgggcgtg tcgagtggcc ttcgagcgta
     3481 tccggctgcg cccgcgaatg ctggttgatg tcagcgtgat tgacacgcgc gcgaacgtgc
     3541 tgggcgagca ggtcgctatg cctgtgctga tcgcgccgac tgccgctcat tgcattgggc
     3601 atcccgacgg cgaatgcgcc accgcgcagg cagccgggag ggcaggcaca ctcatgattg
     3661 ccagcacggt cgcgacacgc tcgctcgaag agattgcgca ggctgccagc ggaccgctct
     3721 ggttccaact ctatgccttt cgcagccgag agttcaccga gcagttggtg cggcgcgcgg
     3781 aagcggctgg ctatcgcgcc atcgttctga ccgtggatct acccaaaccc ggtaatcgcg
     3841 agcgcgaccg gcgcaacaat gtcaccattc cgccggagcc gtatcaagag gccaacttca
     3901 tcggtctaac aagaggagat ttgcctctgg tgtcgctcac ctgggactcc atcgcctggc
     3961 tgcgttccgt tacaacgctt ccactcttga tcaaaggcat actgacggcg gaggatgcgc
     4021 gcctggccgt ggaacacggc gtagatggca ttatcgtctc caatcacggt ggccgccagc
     4081 tcgatagcgc aatccccagc atcgaggcgc tgccagaggt cgtcgaagcg gtaaatgggc
     4141 gctgcgaggt ctatgttgac ggaggtattc gccgggggac cgatatcctg aaggccctgg
     4201 cgctgggcgc gcgcgctgtg ctgatcgggc gtcccatcct ctggggcctg gccgtcaacg
     4261 gcgcggacgg cgtcacccac gtcctggaac tgctccgcca cgaactggag ctatcgatgg
     4321 cgctcgctgg ccgccccacg ctcgccagca tcgaccgctc gctcgtaagc atgtaattgc
     4381 ctcggacgat tcgtagggac agggatgagg gcgttacttt ctcccttttg ccttctgctg
     4441 tatatcgtac agcttgccga tggcctctat cggcgtcagc tcgtcgatgg cgagctgctt
     4501 gatctcctcg atcaggggat gtggctcgga ggagaagagt gtcatttgcg tggaggtggc
     4561 cggaagcatg tcatgcatgg ctttgcggcg ggctttcgcg tcgcctttct gctccagttc
     4621 ggccaggatt tcctcggcgc ggtgaatgac gggcttggga ataccggcca gttgcgcgac
     4681 gtgtacgccg taacttttgt cagccccgcc gggaacaatt ttatgcagga agactacctt
     4741 gccttcctct tcggtgacgg ccacgttcag gcactgaatg cgcggcaggg atttggcgac
     4801 ctcgaccagc tcgtgatagt gcgtggcgaa cagtgtgcgc gcgccacagc gtttgttgtt
     4861 gtgcaggtat tcgatgacgg cgcgggcaat cgataggcca tcgtaggtgc tggtgccgcg
     4921 cccgatctcg tcgaggatta ccaggctgcg cggcgtggcg tgatgcagga tattggccgt
     4981 ttcgaccatc tcaaccatga aggtgctttg tcccgtcgcc aggtcgtcct gcgcgccaat
     5041 gcgggtgaag atgcgatcca cgatgccgat ggtcgccgcc tccgcaggaa cgtagctgcc
     5101 aatctgcgcc atcagcacga tcagcgccac ctggcgcagg taggtcgatt tgcccgccat
     5161 attcggcccg gtgataatca ggatttgcgc cgcgtgattt gagagttcca cgtcgttcgg
     5221 gatgaatggc gtctccgtct gggattgttc gatgaccgga tgacggcccg ccacgatatg
     5281 cacggtatcg ccatcgttga gctgcgggcg acaatagttg tgctgcgcgg caacgttggc
     5341 gaggctgagg tagacatcga tctcggccag cgcgtgcgca gtgtccagga tgcgttccga
     5401 ggcgttgatg gcgatatcgg cgcgcagctg cgcgaagagt tcgctttcca gcttgctgct
     5461 gcgctcctgc gcgttgagaa caagcgtctc gtgttccttc aagtcgggcg tgatgtaacg
     5521 ttcgccgttg gtcagggtct gcttgcgaat ataatcctgc ggcacgcggc ccaggttaga
     5581 gttcgtgacc tcgatgaagt aaccgaagac cttcgtgtag ccaactctga ggttgttgat
     5641 gccggtgcgc ctgcgctcgc gctgctccat ctccgcgatc cacgactgcc cgtgctgcga
     5701 ggcttctttg accgtgtcga gttcttcgct atagccgggt cgcatcacac cgccctccga
     5761 cgtattgagc ggcggctcat caacgatggc gcgttcgatc agggcgatgg tttcgtcgtt
     5821 atccgccagc cgccgcgcca cctgcgccag ggcgggcagc tgctcttcgt tttcgccagc
     5881 aaaggtatcg cgcaggccat tgcgcacctc cgctgcggcc cgaaggccgc tcgccagcgc
     5941 caccaggtct cgcggtgtgg cgatgcgctg ccgcacgcgg ttcgccaggc gctcgatatc
     6001 cccggcgcgt ttcaggccct ccgccagcct tgcctgggcc agcgtgtcgc caagcagttc
     6061 ggcgatcatt tgctggcggt ggcgcaaaat gccgatatcc agcagtggtt gcccaatcca
     6121 gcggcgcagc aagcgaccgc ccataggagt gcgcgtcttg tccagcaccc acaagagcga
     6181 tcctttgacc gtaccgctgc gcccgctctc gaaaagttcc aggttgcgcc gtgtgtgcgg
     6241 gtcgagcgtc atatactcgt tggtgaaata ggtctccagc gaggtaagct ggcgcagcag
     6301 ccccttttgc gtctcctgca cgtaggccag cgcagcgcct gccgcgcgaa tagccagcgg
     6361 caggtgggcg cagccgaatc cctcgagcga tgcgacctcg aaatgcgtca gcaggcgatg
     6421 gcgcgcgtcg tcttccgcga agtagcgcgc gtcgtaaggc gtgacgtggc cggcgacgcc
     6481 cgctagcagc cggaccatcg gcgcggtatc gtcatcatcc tcctcgtcag gctggatggg
     6541 agtcagcgcg ttaccgttac tatggccgtt acctcgctct gacgggttat aggtttcgtg
     6601 agcggctgcc atcgtttcat cgatatcggc gttggcgttg ccgtttgaac cgacgcgcgt
     6661 gaccgttttc tcatacatca ccgccgccag ccagcgcttc ttgcggctgc cctggtggct
     6721 atggcgcgac tcgaccagcg cctcggcggg tgcgatgcgc ccaagctcct gctgtagcgc
     6781 cagttccggt tcgggggtgc tgaactgggt cacggcaaat tcgccggtgg tgatatcaac
     6841 gtaggcgatg ccgactgctt cacgcccgac aaccacgctc gccaggaagt tgttgcgcct
     6901 ggcggccagc attgccgggt cgatcacggt gccaggcgtg acgatgcgca agacctcgcg
     6961 ttcgaccagt ccctttgaga gcgcgggatc gctggtctgc tcgcataccg cgacgcggta
     7021 gccctttgcg accagccgcg cgatgtagcc atcgatggcg tggtgtggca cacccgccat
     7081 tggcgacttc tctccacgcc cccaatcacg ccgcgtcagc gcgatctcga gttcgcgcgc
     7141 cacaatctcg gcgtcatcat cgaacatttc gtagaaatcg cccatgcgaa agaacagtac
     7201 cgcgtcagga ttctgccgct tgatctgcaa atattgcgtc cgtatcggac tatgaacggg
     7261 attctctttg agatagtctt cgaataacac aggaaagcct tcgatactat tgtttcgctc
     7321 agtatggtat gcagcccctc acttgtcaag caaattcctc actttcaaca ttcttcctat
     7381 catcggaaaa aacggcagta caggtgagtt tattgtaagc gagttgtacc gttcacaagc
     7441 gtctcgcgta gcctgttccg ttgcggctga attgtaggag gaaaccgtgc tgttactcat
     7501 actcatcatt tttgcgctag caggagggac gctagcggcg ctggcttatg agaatatttc
     7561 aacgctgtcg atcgatgtgc gcctgacaat ttttggctgg cactcgcccg ctgtagcgct
     7621 gggcgtactg attttgctct cttgcgtcct gggggcgctg ctcctctatg tggtgacgct
     7681 gctgtcggct gtgcgagaac gtcgcgaaat gcgcaggttg cgcaggaggt tggccgagct
     7741 ggaagcgatg caagcgatga tggcggaaca gagagggcag caaggtcaga tggatgtgtc
     7801 acaccagtcg caacaacctc agaccgctca tcagatgatt gtgccaatgc ctggcacaca
     7861 gacatatcgg ctgcaatacc tgtttccacc gcccccaccg aaacgcgaat agcatgaaga
     7921 acggggacgc ttttatcctc tcagcagcct tccgacgagt ctgcgcatgg tgccgtgttt
     7981 cttcgctgag gccagggtca caagcggtac ttcactgtag cgcgcgtcat cttcgatctg
     8041 gcaaggcttg tctggcgtgt tctctggggt gtcaggaggc gtctcgggct gttctgacat
     8101 atcctctgta gtgtcggcag gcgtgtcggg ctgctctggc atgtctggtg gaagcggccg
     8161 cgtaatgcga tttgcccgca gcaccaccgc cgcttgtttc agggtggtat ctgacccgtc
     8221 gtcctggttg tcctcattac tttcgttact ctcatcagtc tcgtccgctt ccgtggagag
     8281 cttttgcaac agcagtgcct cggcttccac ggtatcctcc gagacctc
//
LOCUS       DASZAK010000918         4137 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_663400, whole genome shotgun sequence.
ACCESSION   DASZAK010000918 DASZAK010000000
VERSION     DASZAK010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4137
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663400"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1201
                     /locus_tag="VGT44_16090"
     CDS             128..1201
                     /locus_tag="VGT44_16090"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2582377.1"
                     /translation="MAKDERVQSAIDNWAPRFISNGVDANDFQRVTNSIDVWDEWCQK
                     WSECGVMHEQMAEQAESRGYTVSAAYHYFHAAISYHFGKYLFVHRPQELRAAHEHVVR
                     DYRRALPYFDMPGEHVAMPYEGGASMYGIFRKPAQVARPPVVILVPGLDSVKEELHLY
                     GDDFLRRGMAVLAIDGPGQGEMEFEFPLRHDYEVPVARAIDYLETRPDVDASRVGLMG
                     VSFGGQFAVRAAAFERRLKAVIENCGSYNQSWNFKNRPQISRDTMVHRLKVANEEEAL
                     DRLQHFNLQGVAEKVGCPRLVIQGRRDRLVSAEQGERIAAEAGRMAELWMFEDGNHVC
                     NNIPYKHRPQQADWMREKLYASE"
     gene            1219..2283
                     /locus_tag="VGT44_16095"
     CDS             1219..2283
                     /locus_tag="VGT44_16095"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2582378.1"
                     /translation="MQTEERVQVAIENWAPRFLANGVDPNDFQRITKRVERWDDWSRE
                     WSAVAAMHERMGEEAEAQEYYQSAAYHYLHATITYHFGKFMFVHHPEELRAAHEKVVQ
                     TYRRALPYFDVPGERVEIPYEGGASIPGILRRPWHTPRPPVVVLMPGLDSVKEELHNY
                     GDDFLKRGIAVLAIDGPGQGEMEFDYPIRHNYEVPIHHVIDYLERRPDVNAARVGLMG
                     VSLGGYYAVRAAAFESRVKAAICLAGAANLAEHWERYPILTKEAFTYRSKSANEAATR
                     AFLQSFNLHGVVEKVTSPLLVIMGRLDRLFPAADSEYLAAAAGGPADLMLFEDGNHVC
                     NNIPYKYRPQQADWMHKQLY"
     gene            2331..3896
                     /locus_tag="VGT44_16100"
     CDS             2331..3896
                     /locus_tag="VGT44_16100"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV2582379.1"
                     /translation="MPTESVADWPFQKVLIANRGEIAVRVIRACRDLGLTSVAVYSDA
                     DRNALHVRMADEAYHIGPSQAAKSYLHIPTIIAVAKRAGAQAIHPGYGFLSENAAFVD
                     ACVEAGLIFVGPSAQAQRSVGEKTAARETARAAGVPIVPGAMLDGLDDSGITQLAGQI
                     GYPILLKAAAGGGGKGIRFVLNPGDLPAALRTARSEAKSAFGDDRVYMEKAIAPARHI
                     EVQFIADTHGHIVHLGERECSIQRRHQKLIEESPSPIMDETLRERMTGATINLIRRIS
                     YVNAGTAEFLLGPDRNFYFLEVNARIQVEHPVTEWVTGIDLVREQFRVAAGLPLSFTQ
                     EQVALRGAAIEARISAEDPGNRFLPATGTVQALQEPSGPGVRVDSGLYAGLQVPLFYD
                     PLLSKLIVWGSDRAQAIARLRRALDEYAIIGVRTTIPFARWLTDNPRFLAGDMSTDFI
                     AEEWGKHEHVGAQFIAPDARSPAQIAALVGGLLLNQQIEEEKLRLRPVGAGENGAETS
                     RWKEAGRRESLRR"
BASE COUNT          833 a         1190 c         1303 g          811 t
ORIGIN      
        1 gtgtaggggc gcaattcatt gtgccctcgt tccgggtcat gtgccttccc ctgggcgcaa
       61 ttcattgcgc ccctacacgc catatcgacc ttgacggtgt actacgactg agcgattgga
      121 tgcaaatatg gcgaaagacg agcgtgtgca gagcgctatt gacaactggg cgccgcgttt
      181 catctccaac ggagtcgacg cgaatgattt ccagcgtgtc acgaacagta tcgacgtttg
      241 ggatgagtgg tgccagaaat ggagcgagtg cggagtgatg cacgagcaga tggcggaaca
      301 ggccgagtcg cgcggataca ccgtctcggc tgcctatcac tatttccacg ctgctatctc
      361 ctatcatttc ggcaagtact tgtttgtgca taggccgcag gaactgcgcg ccgcgcatga
      421 gcatgtcgta cgcgactacc ggcgtgcgct gccgtacttt gatatgccgg gagagcatgt
      481 ggccatgccc tacgagggcg gcgcatccat gtatggcatt tttcgcaaac cagcgcaggt
      541 cgccaggccg ccggtcgtga ttctggtgcc tggcctcgat tcggtcaagg aagaattgca
      601 tctctatggt gacgactttc tgcggcgtgg catggcggtg ctggctatcg atgggccggg
      661 acagggtgag atggagttcg agtttcccct gcgacacgat tatgaagtcc ccgtggcgcg
      721 cgccatcgat tatcttgaga ccaggccgga tgtggacgca agccgcgtcg gcctgatggg
      781 ggtgagcttc ggcggccagt tcgcggtgcg cgcggcggcc ttcgagcgcc ggttaaaggc
      841 ggtcattgag aactgcggat cgtataacca gtcctggaac ttcaaaaatc gcccacagat
      901 cagccgcgat acgatggttc accgcctcaa agtggccaac gaggaagagg cgcttgatag
      961 attgcagcat ttcaacctgc aaggtgtggc cgaaaaggta ggctgtcccc ggctggtcat
     1021 tcaaggccgt cgcgaccggt tagtatcggc ggagcagggc gaacgcatcg cggcagaagc
     1081 cggtcgaatg gctgaactct ggatgttcga ggacggcaat cacgtctgca ataacattcc
     1141 ctacaaacat cgaccgcagc aggccgattg gatgcgcgag aagctctatg ccagtgagta
     1201 agaaaggacg aaccattcat gcaaaccgaa gaacgggtac aggtcgcgat tgaaaactgg
     1261 gcgccgcgtt ttctcgccaa tggcgtcgat ccgaacgatt tccagcgcat cacaaagcgc
     1321 gtggagcgtt gggacgattg gagccgcgaa tggtccgcgg tcgcggcaat gcatgaacgc
     1381 atgggcgagg aggccgaggc gcaggagtac taccaatcgg cggcctatca ctatctccac
     1441 gcgacgatta cctatcattt tggtaaattc atgtttgtgc atcacccgga ggaattgcgc
     1501 gccgctcatg agaaggtcgt gcagacctac cggcgcgccc tgccgtattt tgacgttccc
     1561 ggcgagcgag tcgagatccc ttacgagggc ggggccagca tcccaggcat tttgcgcaga
     1621 ccctggcaca cccccaggcc gccggtcgtg gttctgatgc ccggcctcga ctcggtcaaa
     1681 gaagagctgc acaactacgg ggatgacttc ctgaaacgcg gcatagcggt gctggctatc
     1741 gatgggccgg gacagggcga aatggagttc gactatccca ttcgccacaa ttatgaggtt
     1801 cccattcacc acgtgatcga ctatcttgag cgccgccccg atgtcaatgc cgctcgcgtt
     1861 ggtctgatgg gggtgagcct cggcgggtac tacgcggtgc gcgcggcggc cttcgagtcg
     1921 cgcgtgaagg cggcgatctg cctggccgga gccgcgaacc tggcggaaca ctgggagcgc
     1981 taccccattc tcacgaagga agccttcacc tatcgcagca agtcggccaa tgaagcggcg
     2041 acgcgcgcct ttttgcaatc gttcaatctg cacggcgtag ttgagaaggt cacatctcct
     2101 ctgctcgtta tcatgggtcg cctggacagg ctcttcccgg cagcagattc ggaatacctg
     2161 gcagcagcgg caggcggccc agcagacctg atgctgttcg aggacggcaa ccacgtctgc
     2221 aacaacatcc cctacaaata tcgcccgcag caggccgatt ggatgcacaa gcagctctat
     2281 taagtagcac atgctatact tatgtagatt gatgaaggag ctgattacct atgcccacag
     2341 aatctgttgc ggactggccg tttcagaagg tcctgatcgc caatcggggt gagatcgccg
     2401 tgcgggtcat tcgcgcctgt cgtgacctgg gattgaccag cgtggccgtc tatagcgatg
     2461 ccgaccgcaa tgccctgcat gtgcgtatgg cggatgaggc ctaccatatt ggcccgtccc
     2521 aggcggcgaa gagctatttg catattccga ccatcattgc agtggcaaaa cgggcggggg
     2581 cgcaggccat tcatcccggc tacggtttcc tttcggagaa tgccgcgttt gtggatgcct
     2641 gcgttgaggc ggggctgatc ttcgtagggc cgtcggcgca ggcgcagcgc agcgtaggcg
     2701 aaaagaccgc ggcgcgcgaa accgctcgcg ctgctggtgt gccgatcgtg ccgggcgcga
     2761 tgctggacgg gctggatgac agcggcatca cgcagcttgc gggacagatc ggctatccca
     2821 tcctgctcaa ggcggcagcc ggtggcggcg gtaagggcat tcgctttgtg ctgaacccag
     2881 gtgacctgcc cgccgcactg cgtacggctc gcagcgaggc caaaagcgct ttcggcgatg
     2941 accgagtcta tatggagaag gccatcgccc cggcgcgcca tatcgaggtt caatttatcg
     3001 ccgacacaca cggccatatc gtccacctgg gcgagcgcga gtgcagcatc cagcgccgcc
     3061 atcagaagct gatcgaggag tcgccttcgc ccatcatgga tgaaaccctg cgcgagcgca
     3121 tgaccggcgc gacgatcaac ctgatccgca ggatttctta cgtcaatgcc ggaaccgccg
     3181 agtttctgct gggtcccgac cgcaatttct atttcctcga agtcaacgcg cgcatccagg
     3241 tggagcaccc cgtcacggaa tgggtgacgg gcatcgacct ggtgcgcgag cagttccgcg
     3301 tggcggctgg cctgcccctc tcgtttaccc aggaacaggt ggctctgcgc ggggcggcca
     3361 tcgaagcccg tatctccgcc gaggaccccg gcaaccgttt cttgcccgct accggcaccg
     3421 tgcaggctct gcaagagccg tccggcccag gcgtgcgcgt cgatagcggc ctgtacgccg
     3481 gtttgcaggt tcccctgttc tatgaccctc tgctctccaa gttgatcgtg tggggcagcg
     3541 accgcgcgca ggcgattgcc cggctgcgcc gcgccctaga tgagtatgcg atcatcgggg
     3601 tgcgcaccac catccccttc gcgcgctggc tcacggataa ccctcgcttt ctcgctggcg
     3661 acatgtccac cgattttatc gccgaagagt ggggcaagca tgaacatgta ggggcgcaat
     3721 tcattgcgcc cgacgcgcgt tcccccgcgc agatcgccgc gctggtgggc ggcttgctgc
     3781 tcaaccagca aatcgaagag gagaaactac gactcaggcc ggtgggagcg ggcgaaaacg
     3841 gcgcggaaac gagccgctgg aaagaggctg ggagacggga gtccttgagg cggtgaaaat
     3901 gcgagggtat gatgatattt aatgaccggg tctggcaatg tcaccctgag cgcagcgaag
     3961 ggtctggctc tatggaacgc gagatgctga gcgcagcgaa gcatgacatt gctggcttca
     4021 gttgataaaa atcattataa caggcagaga tttcggtcgg ggcgaccgag gtcacggatg
     4081 taccgccgcg aagacgcttg aatagtttcc tagcaggcca aagttgcctt cgtcgaa
//
LOCUS       DASZAK010000919         6213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_165680, whole genome shotgun sequence.
ACCESSION   DASZAK010000919 DASZAK010000000
VERSION     DASZAK010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6213
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165680"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VGT44_16105"
     CDS             complement(<1..576)
                     /locus_tag="VGT44_16105"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2582380.1"
                     /translation="MARTPVLDALQQLSRDFVEADAQHISVEQIQEQRRSPIRIRRRD
                     LLKGAGVAAAGLALVNPLHWGQGVLSASASGSPRIGIIGAGIAGLNAALTLHDAGLAA
                     TVYEVSGSIGGRIHSNTTTWAENQVTEWCGELIDTGHTAMQGLAKRFGLPLVDLLKAQ
                     PDGAQTTYFFFGKYYTQAQANEDFKPVYQVLK"
     gene            complement(596..2248)
                     /locus_tag="VGT44_16110"
     CDS             complement(596..2248)
                     /locus_tag="VGT44_16110"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEV2582381.1"
                     /translation="MSFTFTSSAADWGRIAPELVLAAFALLMLMVDLTLPQPGKDKQP
                     GNFIVLPVLGLLGVVGALIASIILFNIGDYQTAFNNMVGSDAGSLYAYFIILSASSLG
                     IALSPAYLKRLKLIHQGEYYALMLLGTLGMMIMAAATSFLTIFLGLEMLSLALYILCG
                     FARRGASQESGMKYFLLSSFASAILLYGIALIYGATGNTSFAGIHTFIAAHTNATLLL
                     LIGMGLLIVGFAFKVSAVPFQAWTPDVYDGAPAPVTAFMSVGTKAAALIAFARVFDIV
                     LGPLANQWLPVVWAIAVLTIVGGNILALAQSNLKRLLAYSSIAHAGYLLIGVVAGGTV
                     GIAAILFYLFCYSFLNLGAFGIVSVLERADNSGSSLTDLRGLWYRQPLLAGLLAFFLL
                     ALAGFPPMAGFAAKYYIFYAALQSSHPELLIIGVLASVLGMYYYLRFIAAMFMERQPA
                     AQAATTTASAPGPTPVSGRRVSAKLSNGGAATAVAVKATTSPLVKDKAAAPMEEPRAV
                     IGWTTWLALGVSVAGTLLMGTLLAYWLVELAQYAAGVMMRVM"
     gene            complement(2258..3826)
                     /locus_tag="VGT44_16115"
     CDS             complement(2258..3826)
                     /locus_tag="VGT44_16115"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEV2582382.1"
                     /translation="MFPLLSLIVFLPLIGGIVVLLLPGERFNLIRWIALAFAWVDLLV
                     VLGLALAYSQAVSGFAASIVGASPTATYFSTVHEQVSWIVTQGFSLNYSLDVDGISLL
                     LVALTSLLTVVCIGASFRIDQKVKNYMAFMLLLESGMLGVFLASNLFLFYIFWELMLI
                     PAYFLVGSWGGERRIYAAVKFVLYTSVGSLLMLAGIIALGYYHQQLVPGSAYSLDLAT
                     LLNGRLDSTTQMWLFLAFAAAFAIKVPLVPFHSWLPDAYSEAPAPVSAMLAGAMSKTG
                     AYGFLRFGILLFPNAAHTLAPLINTLAVVGILYCAWMALAQSDVKRLLGYSSISHLGI
                     VMLGMFAFNAQGIEGSVLQMVNHGITTAALFLIVGYIETRTGTRRLGDYGGLARRIPI
                     LATVMLIAALSSLGLPGLNSFAGEFLALLGAFQSSSLFGLLGTAVVVPAAWYMMRFFQ
                     GMMEGPRKVDGPMVALERKNALSDIQLGEFMAIVPLLILIFYIGFQPQPLTFLQEPTV
                     VNMLQHIGGILIHG"
     gene            complement(3831..5888)
                     /gene="nuoL"
                     /locus_tag="VGT44_16120"
     CDS             complement(3831..5888)
                     /gene="nuoL"
                     /locus_tag="VGT44_16120"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEV2582383.1"
                     /translation="MTNLTAWVLLFPLLGFIILGLGGRGMSRAAIVTVALGATGLAFF
                     CAAISFLSMLGSPPSSASAGDQVLYTWVTSGVVGAAPGTTAFSVSFGLLFDQLSATML
                     MVITGVGFLIHIYSVGYMEDDPGFWRFFAYMNFFIFAMTLLVSADNFLFLLVGWALVG
                     LASFLLIGFWYTRPAAVAAARKALVINIIGDFGLMIAIFLIFKHYHTLNYFTNGSGTI
                     GVFNQLPGDATTSILTIICLLLFVASAAKSAQLPLYMWLPDAMEGPTPVSALIHAATM
                     VTAGVYLVARANQFFELSPTTLLAVAVIGGITALFAATIAIFQLDIKRVLAYSTISQL
                     GYMFMGEGAHNYGAGIFHLTTHAYFKALLFLGAGAVIHALGGEQDIRQMGGLRSRLKI
                     TFWVFLIAALAISGIPPLSGFFSKDEILSSLLAQASGGAGTVYYALWGIGLLTAGLTA
                     FYMFRLTLGIFAGRYRGSGPLAHGHGEEEESEEGEGAHNVGRGGLYAVNEAPSVMTVP
                     LIVLAVLSVIGGLVGSFALFGAAKWHPLANFLNPVFTNSSWVTKAVAIPAPSLSVEWI
                     STGISIGLGVLGILGALALYRKGFNYKESRNPLYQLVFHKFYVDEFFIAILINPLLDL
                     GGAFSRYFEGNVLDGGSRGVGWVLSRTSGGLRRLQTGYMRNYALAILLGVVLIVLYYA
                     VRG"
     gene            complement(5894..>6213)
                     /gene="nuoK"
                     /locus_tag="VGT44_16125"
     CDS             complement(5894..>6213)
                     /gene="nuoK"
                     /locus_tag="VGT44_16125"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEV2582384.1"
                     /translation="LEAIMAIPVSWYLIVSACIFTIGVAGVLVRRNPLIIFMSIELML
                     NAVNLSFIAFSHYLNSVDGQMFVFLVLTVAAAEVVVGLAIIVSIFRRRRNIDVDDMNV
                     LRG"
BASE COUNT         1473 a         1907 c         1815 g         1018 t
ORIGIN      
        1 tttcagtacc tggtacaccg gcttgaaatc ttcgttggcc tgcgcctgtg tataatactt
       61 cccaaagaaa aaataggttg tctgcgcccc atccggctgc gccttcagca gatcgaccag
      121 tggcaatcca aagcgtttcg ccagtccctg catcgccgta tggccggtgt cgatcagttc
      181 gccacaccat tccgtgacct ggttctccgc ccacgtcgtc gtgttagaat ggatgcgtcc
      241 cccaatactg ccggagacct cgtacaccgt cgctgccaat cccgcgtcat gcagtgtcag
      301 cgcggcattc aacccggcga tacccgcgcc gatgatgccg atgcgcggcg acccactggc
      361 cgaggcgctc aacaccccct ggccccaatg caaaggattg acgagcgcaa gcccggcagc
      421 cgccacgccc gctcccttca acaggtcacg cctgcggatt ctgatggggc tgcgccgctg
      481 ctcttgtatc tgctcaaccg aaatatgttg tgcatccgcc tccacgaaat cgcgagagag
      541 ctgctggagc gcgtccagga caggtgtgcg agccattgta tctcctttct tgtccctaca
      601 tcacccgcat catcactccc gccgcgtact gcgccagttc caccagccag taggccagca
      661 gcgtccccat caacagcgtc ccggcgaccg acacacccag cgccagccag gtcgtccagc
      721 cgatcaccgc gcgcggctcc tccatcggcg cagccgcctt atccttgacg agcggcgagg
      781 tcgtcgcttt gacagcgact gccgttgcag cgccgccatt gctcagcttc gcggagacgc
      841 gcctgccgct aacgggagtc ggccctggcg cgctggctgt cgtcgttgcc gcctgcgccg
      901 cgggctggcg ttccatgaac atcgccgcga tgaagcgcag gtagtagtac atgcccagca
      961 cgctggccag cacaccgatg atgagcagtt cgggatggct gctttgtaac gcggcgtaga
     1021 agatgtagta cttggcagca aatccggcca tcggcgggaa acccgccagc gccagcagga
     1081 agaaggccag cagtccggcc agcagcggtt ggcgatacca taggccgcgc aaatcggtca
     1141 ggctactgcc ggaattgtca gcgcgttcca gcacggagac gatgccgaac gcgcccaggt
     1201 tcagaaagct gtagcagaag agatagaaga ggatggccgc gatgccgacc gtcccgcccg
     1261 cgacgacgcc gatcaacagg tagccggcgt gggcgatgct cgaataggcc agcagccgtt
     1321 taaggttgct ttgcgccagc gcgagaatat tgccgcccac aatcgtcagg acggcgatag
     1381 cccacacaac cggcagccac tggttcgcga gcgggccgag gacgatgtcg aagacgcgcg
     1441 cgaaggcaat cagcgcggcg gcttttgtgc caaccgacat aaaggccgtc accggcgcgg
     1501 gcgcgccgtc gtagacatcg ggcgtccatg cctggaacgg cacggctgag accttgaagg
     1561 cgaacccaac gataagcaga cccatgccga tcaagagtaa gagtgtggcg ttggtatgcg
     1621 ctgcaatgaa tgtatggatt ccggcaaacg aggtattgcc cgtcgcgcca taaatcaagg
     1681 caatgccgta gagcaaaatg gccgaggcga acgaactgag caggaaatat ttcatgcccg
     1741 attcctgcga agcgccgcgc cgcgcgaagc cgcacaggat gtagagcgcc agcgagagca
     1801 tctccagccc caggaagatc gtcaggaagc tggtcgcggc agccataatc atcataccga
     1861 gcgtaccgag cagcatcagc gcgtaatact cgccctggtg gatcagcttc aggcgtttca
     1921 ggtaggcggg cgagagcgcg atgcccaggc tacttgcgct cagaataatg aaataagcgt
     1981 acaatgaccc tgcatcagag ccaaccatgt tgttaaaggc cgtctggtag tcgccaatat
     2041 tgaacagaat gatactggca atcaatgccc ccactacacc aagcagtccc aaaacaggca
     2101 gcacgataaa attgcccggc tgtttatcct tgcccggctg cggaagcgta agatcaacca
     2161 tcagcatgag cagcgcgaag gccgccagca ccagttccgg cgcgatacgc ccccaatcgg
     2221 cggcggatga tgtaaatgtg aacgacataa tcaaacctca tccatggatc aatattcctc
     2281 cgatatgttg gagcatgttc accaccgttg gctcctgcaa gaaggtcagc ggctgtggtt
     2341 gaaaaccgat gtaaaagatc aggatcagca ggggcacgat ggccataaac tcaccaagct
     2401 ggatatcgct cagcgcgttt ttgcgttcca gcgcgaccat cggcccatca actttgcgcg
     2461 gcccctccat catcccctgg aaaaagcgca tcatgtacca cgcggcaggt accacaacgg
     2521 cggttcccag caagccgaag agcgaactgc tctggaatgc gcccagcagc gccaggaact
     2581 cgcccgcgaa gctgttgagt cccggcaaac caagcgacga aagcgccgcg atcagcatga
     2641 cggtggccag gatgggaatg cgccgcgcca gcccgccata atcgcccagg cggcgtgtgc
     2701 cggtgcgcgt ctcgatatag cccacgatca ggaagagcgc ggcggtggta atgccgtgat
     2761 tgaccatctg caagaccgaa ccttcgatgc cctgtgcgtt gaaggcgaac atacccagca
     2821 tgacgatgcc caggtggctg atgctggaat acccaagcag ccgcttgaca tcgctctgcg
     2881 ccagcgccat ccacgcgcag tagagaatgc ccacgaccgc cagcgtattg atcagcggcg
     2941 cgagcgtgtg cgcggcattc gggaacagca ggataccgaa gcgcagaaag ccatacgccc
     3001 ctgtcttgga catcgctccc gccagcatcg cagaaacggg cgcgggcgct tcgctatagg
     3061 catcgggtag ccagctatgg aacggcacaa gcggcacttt gatggcgaag gcggctgcga
     3121 aggccaggaa cagccacatc tgcgtggttg aatcgagccg gccattgagc agcgtcgcca
     3181 ggtcgagcga ataggcgctt ccaggcacaa gctgctggtg gtagtagccc agcgcgatga
     3241 tgcccgccag catcagcagg ctgccgacgg aagtgtagag cacgaacttc acagcggcat
     3301 agatgcgccg ttcgccgccc caactgccga ccaggaaata ggccggtatc agcatcagtt
     3361 cccagaaaat atagaacagg aagagattcg aggccaggaa aacgcccagc attccgcttt
     3421 ccagcagcag catgaaggcc atgtagttct tgaccttctg atctattcgg aatgacgcgc
     3481 cgatgcacac aaccgtcagc agcgaggtta gcgccaccag cagcaggctg atgccgtcta
     3541 catccaggct gtaattcagg ctgaatccct gagtaacaat ccagctcacc tgttcatgga
     3601 cggttgagaa ataagtagct gtcggacttg cgccgacaat ggaggcggcg aagcccgaca
     3661 cggcctgcga ataggccagc gcgaggccca gcacgaccag caggtctacc cacgcgaagg
     3721 ccagcgcgat ccagcggatc aggttgaacc gctcgccggg cagcaggagg acgacgatgc
     3781 cgccgatcaa cggcaggaag acaatcagcg ataacaatgg aaacatgccg ctaccccctc
     3841 accgcgtaat acagaacgat cagcacgacg cccagcagga tcgccagggc ataattgcgc
     3901 atatagcccg tttgcaggcg gcgcagcccg ccactggtgc ggctcaagac ccagccgacg
     3961 ccccggctgc ccccgtccag cacgttgccc tcgaagtaac gcgagaacgc gccgcccagg
     4021 tcgagcagcg ggttgatgag gatcgcgata aagaactcat cgacgtaaaa cttgtggaaa
     4081 accagttgat agagcggatt gcggctctcc ttgtaattga agcccttgcg atagagcgcc
     4141 agcgcgccca ggatgcccag cacaccgagt ccgatgctaa tgcccgtcga gatccactcg
     4201 accgaaagcg agggcgcggg gatggcaacc gcctttgtga cccaggatga attcgtaaag
     4261 acgggattca ggaaatttgc caggggatgc catttcgccg cgccgaaaag cgcgaacgag
     4321 ccgaccagcc cgccgattac cgagagaacc gccagcacta tcaacggaac tgtcatcacg
     4381 gagggcgctt cgttgaccgc gtagaggccg ccgcgtccca cattgtgcgc cccctcaccc
     4441 tcttcactct cttcttcctc gccgtgtcca tgcgcgagcg ggccgctgcc gcgatagcga
     4501 ccggcaaaga tgcccagcgt caaccggaac atgtagaaag ccgtcagccc cgccgtcagc
     4561 aggccaatgc cccacagcgc ataatagacc gtgccggcac cgccgctcgc ctgggccagc
     4621 agcgaactca ggatttcatc cttgctgaag aagcccgaca gcggcggtat gccgctgatc
     4681 gccagcgccg cgatcaagaa gacccagaag gtgatcttga gacggctgcg cagcccgccc
     4741 atctggcgga tgtcctgttc tcctcccagc gcgtggatta ccgcccccgc gcccaggaag
     4801 agcagcgcct tgaaataggc gtgtgtggtc aggtggaaga tacccgcgcc atagttgtgc
     4861 gcgccctcac ccatgaacat gtagccaagt tggctgatgg tggaataggc cagcacgcgc
     4921 ttgatatcga gctggaagat cgcgatggtg gccgcgaaga gcgccgtaat gccgccaatc
     4981 acggccaccg ccagcagcgt ggtgggcgac agctcgaaga actggttggc gcgcgccaca
     5041 agatagacgc ccgccgtgac catcgtagcg gcgtggatca gcgcgctgac tggcgtaggg
     5101 ccttccatcg cgtcgggcag ccacatatac aggggcagtt gcgccgattt cgccgcgctc
     5161 gccacaaaga gcagcaggca gataatcgtc aggatggaag tcgtggcatc gccgggaagc
     5221 tggttgaaga cgccgatcgt gccgctgccg ttggtaaaat aattgagcgt gtgatagtgc
     5281 ttgaaaatca ggaaaatggc gatcatcagg ccaaagtcgc cgatgatgtt gatgaccagc
     5341 gctttcctgg ctgccgcgac cgccgctggc cgtgtatacc agaagccgat cagcaggaac
     5401 gacgccaggc cgaccagcgc ccaaccaacc agcaagaaga ggaagttatc cgcgctgacc
     5461 agcagcgtca tggcaaaaat aaagaagttc atataggcga agaagcgcca gaagccgggg
     5521 tcatcttcca tgtagccgac ggagtagata tggatcagaa agcccacgcc cgttatgacc
     5581 atcagcatcg tggcggagag ctggtcgaag agcaggccaa agcttacaga gaaggcagtc
     5641 gtgcctggcg cggcccccac gacgccggac gtgacccagg tatagagtac ctggtcgcct
     5701 gctgatgctg aggagggcgg cgaaccgagc atcgaaagaa agctgatggc ggcgcagaaa
     5761 aaggccaggc cggtcgcgcc caatgccacc gtcactatcg cggcgcgtga catgccgcgc
     5821 ccgcccagcc ccaaaatgat gaaaccgagc aggggaaata acaataccca tgcggtaaga
     5881 ttagtcatac tacttagcct cgcaatacat tcatatcatc tacatcgatg ttgcggcgac
     5941 ggcggaagat ggagacgatg atcgccagcc ccaccacaac ttctgctgct gcaactgtca
     6001 gcaccaggaa gacaaacatc tgcccgtcaa cagagttgag atagtgcgag aaggcgataa
     6061 acgagaggtt gacggcattc agcatcaatt cgatcgacat aaaaataatc aggggattgc
     6121 gccgcacaag gacacccgcg actccgatgg taaaaataca cgcgctaacg attaaatacc
     6181 atgagacagg tatagccatg attgcctcca gat
//
LOCUS       DASZAK010000920         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_555436, whole genome shotgun sequence.
ACCESSION   DASZAK010000920 DASZAK010000000
VERSION     DASZAK010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555436"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VGT44_16130"
     CDS             <1..287
                     /locus_tag="VGT44_16130"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEV2582385.1"
                     /translation="LEVVTNTMFASPSYKRYGGNIAHNSYEPGFKLTLGLKDVNLALE
                     AAKAKQALLPAAEIVRDSMLEAVEQGLGSKDWSALAKVTRRRAGLKEAEA"
     gene            complement(367..1746)
                     /locus_tag="VGT44_16135"
     CDS             complement(367..1746)
                     /locus_tag="VGT44_16135"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEV2582386.1"
                     /translation="MTQTQEPSVSTGDTPFVPIEKIVSLCKRRGFVYPNSEIYGGVSG
                     IYDFGPLGVELRNNIRRYWWWSMVQTNDNVVGIEGAIITHPRVWEASGHVENFVDRLV
                     DCKNCKRRFRYDHLPEEDVRQNRCLACGGELTEPRTFNLMMETYIGVLEGERTKTYLR
                     GEASQNIYLDYDNVLKSSRMSIPFGICQIGKAFRNEVTPGNFLFRQREFEQWDLQFFV
                     HPSEMQQWFDYWKERRFAWYQSIINHKDRLRFRAHGADELAHYAKQAFDIEYQTVLGW
                     QEWEGIHWRQDWDLSRHSEYSGHDLSYTDPETKERYLPWIVETSGGVDRTFLNLLIDA
                     YEEEPPSGKDKEGRTVLHLNPMLAPVKVAVLPLQKNKAELVTTAKELHSKLRRLMVSR
                     YDDVANIGRRYRRQDEIGTPFCVTIDFTTLEDQTVTVRERDSMSQTRLHLNEVPAYLL
                     ERVRWDYSV"
BASE COUNT          428 a          613 c          634 g          458 t
ORIGIN      
        1 tcctagaggt cgtcaccaac actatgtttg cgagcccaag ctacaagcgc tatggcggca
       61 atattgccca caacagctac gaaccagggt tcaagctgac cctgggcctc aaagacgtca
      121 acctggcgct cgaggcagcc aaagcgaaac aggctctgtt gcctgctgcg gaaatcgtgc
      181 gtgattcgat gctcgaagcg gtcgagcagg gactgggctc taaggactgg tcagctctgg
      241 cgaaggtgac acgtcgccga gcaggcttaa aagaagcaga agcctagagg gaagatcctc
      301 gtagattgcc gattttgatc gtcaaaattc cgtcgggacg cccgcaccgc gggcgtcccg
      361 agaaaatcac acactgtaat cccacctgac ccgctccagg aggtaggcag ggacttcgtt
      421 caggtgcagg cgggtctgcg acatgctgtc gcgctcgcgc accgtcacgg tctggtcttc
      481 cagcgttgtg aagtcgatgg tgacgcagaa gggggtgccg atctcgtcct ggcgccgata
      541 gcgtcggcca atgttggcca cgtcgtcgta ccgcgagacc atcaggcggc gcagtttgga
      601 atgcagctcc ttcgcggtcg tcaccaactc tgccttgttc ttttgcaatg gcagcacggc
      661 caccttgacc ggggcgagca tggggttgag gtgcagcacg gtgcggccct ctttgtcctt
      721 gcctgatggc ggctcctcct cgtaggcgtc gatgagcagg ttgaggaagg tgcgatctac
      781 gccgcccgag gtttcaacga tccagggaag gtagcgctct ttcgtttcgg gatcagtgta
      841 actgagatca tggccgctgt actcgctgtg gcgtgagagg tcccagtcct gccgccagtg
      901 gatgccctcc cattcctgcc agcccagcac ggtctggtat tcaatgtcga aggcctgctt
      961 cgcgtagtgc gccagttcgt ccgccccgtg cgcgcggaag cgcaggcggt ctttgtggtt
     1021 gatgatgctc tggtaccacg cgaaccgccg ctccttccag tagtcgaacc actgctgcat
     1081 ctcactcgga tggacgaaga attgcaggtc ccactgctcg aactcccgct ggcggaagag
     1141 gaagttgcct ggcgtgacct cgttacggaa ggccttgccg atctggcaga tgccgaaagg
     1201 gatcgacata cgcgaggatt tgagcacgtt gtcgtagtcc aggtagatgt tctggctggc
     1261 ttcgccgcgc aggtaggtct tggtgcgctc accttcgagc acgccgatgt aggtctccat
     1321 cattaggttg aacgtgcgtg gctcggtcag ttctcccccg caggccaggc acctgttctg
     1381 gcgcacgtcc tcttcaggca ggtgatcgta gcggaaacgg cgcttgcagt tcttgcagtc
     1441 cacaaggcgg tcaacgaagt tctcgacatg cccgctggcc tcccacacgc gcggatgggt
     1501 gatgatggcg ccttcgatgc caaccacgtt gtcgttggtc tgcaccatcg accaccacca
     1561 gtagcggcgg atattgttgc gcagttcgac gcccagcggc ccgaagtcgt agatgccgga
     1621 gacaccaccg tagatttcgg agttggggta gacgaagccg cgccgcttac acagcgagac
     1681 gatcttttcg atagggacaa acggggtatc cccggtggac acggatggtt cctgtgtctg
     1741 tgtcatgggt ggttcctcct cgcacaaatg atcccggacg tttgcaacac caacgtccag
     1801 ggacgaattc atgcctgtgc tggtggtaaa ttgcgttctt cgtctcagac taactgaccg
     1861 ttggaactct tccacgatcg tttctgcaag gaacgcattt tctcgcctgc gccgacctgg
     1921 ttctcgcttg cgccaggcat agtgtccact cacagaaatc tccagcaccg aacacatgag
     1981 cacaacgaaa atactcattc atgtgcaaag caatgaactg agaggtcatc agtggtcctg
     2041 cgagaaaatg ctgagcgctt ctcctctcaa agaaggcaag agagcgaaca tgctcatggc
     2101 aagcaggacc acatttatgg tcgcatttgt gcc
//
LOCUS       DASZAK010000921         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_663581, whole genome shotgun sequence.
ACCESSION   DASZAK010000921 DASZAK010000000
VERSION     DASZAK010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663581"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VGT44_16140"
     CDS             complement(<1..1067)
                     /locus_tag="VGT44_16140"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2582387.1"
                     /translation="MAIEQLVLGGEHMPAADGRTFAVIEPGTGKPFAEVAQAGVEDAE
                     RAVQVAHHAFAEGRWPRLSATERGRVLLRASTLVRERLEELANIESRNAGKPVRDARG
                     EIGLVADVFEYWGGAANKIFGETIPVQDAGLEVTLREPVGVCALITPWNFPLVIASWK
                     IAPALACGNTVVVKPAQLTPLSVLKLADILMEAGLPEGVLSVLPGPGAAVGNALATHP
                     LVAKVSFTGSTEVGSQIMRLCADNITRVSLELGGKAANVVFADTDLERCIESSVFSVF
                     GNSGQDCCARSRLLVERSMYDEFTARFSQRTAALVIGQPLDEASEIGPLISAGQRQRS
                     LDYVALGRAEGAKLVSGGAIP"
     gene            1526..2002
                     /locus_tag="VGT44_16145"
     CDS             1526..2002
                     /locus_tag="VGT44_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582388.1"
                     /translation="MSSQGNLSTRARILQLFGYEVGGQNSPLWTNPQNFSLERDRETF
                     NNWKNRKRSFIHEEIRDSAWVSFREQGIALVVRCHPDESMTVANLFHQDRQFKGFWHL
                     IDSGIDGMRVLQLDLQNNFPLIIVAIRESQIHSGIGFVRGGAQAYFRVIQLHKPGK"
     gene            complement(2008..>2655)
                     /locus_tag="VGT44_16150"
     CDS             complement(2008..>2655)
                     /locus_tag="VGT44_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582389.1"
                     /translation="YLPRTWLDYSRVLLTGYYPNSASPQQNVYLLDTNKGANQQSSDL
                     QLLATVGTQQNVCWDFDSSFDGKTVYINQCNSTSSSSSSTVEAQQTTGSAAPTTVFSS
                     STQAITNVRVFEKSDAFILAATTTGLYKIAVDGTNNAIQLTSVNQGTVGLNRYSQYFW
                     SNVSRDDSLYALESYTPGQNTTYNLFFGSMNGSAAQSFATISNGTELEIVGWTTM"
BASE COUNT          567 a          755 c          752 g          581 t
ORIGIN      
        1 tccggaatag cgcctccgct caccagtttt gccccttccg cgcggcccag cgctacgtag
       61 tcgagcgagc gctggcgctg cccggcggag atgagcgggc caatctcact cgcctcgtcc
      121 agtggctgcc ctatgaccag cgctgccgtg cgctggctga aacgcgccgt gaactcatcg
      181 tacatcgagc gctccaccag caggcgcgag cgagcgcagc agtcctgccc gctattgcca
      241 aagacggaaa agaccgacga ttcgatgcag cgctccaggt ctgtatcggc gaagaccacg
      301 ttggccgcct tgccgcccag ttcaagcgag acgcgcgtga tgttatcggc gcataggcgc
      361 atgatctgcg atcccacctc ggttgacccg gtgaacgaca ccttcgccac aaggggatgc
      421 gttgccaggg cattgccgac cgccgcgccg gggccaggca gcacggagag gacgccctcc
      481 ggcaaaccgg cctccatcaa aatatcggcc agcttcagca cggacagtgg cgtaagctgg
      541 gctggtttga ccacgacggt attgccgcag gccagcgcgg gcgcgatctt ccacgaggcg
      601 atcacgagcg gaaagttcca gggcgttatc agggcgcaca cgccgaccgg ctcgcgcagc
      661 gtcacctcca gtccggcatc ctgtacaggg atggtttcgc cgaaaatctt gttggccgcg
      721 ccgccccagt actcgaagac atcggcgacc agcccgatct cgccccgcgc gtcgcgcaca
      781 ggcttgcccg cgttacgcga ctcgatgttg gccagttctt ccagccgctc gcgcaccagc
      841 gtcgaggcgc gcagtagcac acgcccgcgt tctgtcgcgc tgagtcgcgg ccagcgtccc
      901 tccgcgaagg cgtgatgggc aacctgaacc gcgcgttcgg catcctccac tcccgcctgg
      961 gcgacctcgg cgaacggctt gcccgtcccc ggctcgatca ccgcgaatgt cctgccatct
     1021 gctgcgggca tatgttcccc gcccagcact aactgctcga tagccatcgg tatgctcctt
     1081 ctgttaagac tagcacgttg ataagacccg gcatggcgat cccttgcggt cgccatgctg
     1141 tccatcctga ctaataaata gccctgctat ctcatcataa agtatatgct atcgaggctt
     1201 caatgtacga aacttccgta actactcgaa accagctgac acttttggca tattttaatg
     1261 aagattaacc gtcacagccg gtcatacgtg gttgtcatcc tgagcgcagc gaaggatctg
     1321 gctcgatggt ggcatcgtag tatggtccga agatccttcg ctgcgctcag gatgacaggg
     1381 ctgaacttga tcggtaacat tcatcagaat atatgcgcat atatcttctc tgcctggttg
     1441 tcttctcatt tgccggtaga aaccgtttga tgctacgatg agtgaataga attgggaatg
     1501 tattgcaaac aataaggaga aaggcatgag ttctcaagga aatctgtcaa cacgagcgcg
     1561 aatcctacaa ctctttggct acgaggtcgg cggacaaaac tcaccactgt ggaccaatcc
     1621 gcaaaacttt tcattagagc gtgacagaga gacctttaac aactggaaga atagaaaaag
     1681 aagcttcatc catgaggaaa ttcgcgattc agcctgggta tcatttcgag agcagggaat
     1741 tgcgctggtg gttcgttgtc atcctgatga gagcatgaca gtagcaaatc tcttccatca
     1801 ggatagacaa ttcaaggggt tctggcacct cattgattct gggatagatg gtatgcgtgt
     1861 gctgcaattg gatttacaaa acaactttcc gctgattatc gtggcgatcc gcgaaagcca
     1921 gattcattca gggattggtt ttgtcagggg cggcgcacag gcctatttca gggtcattca
     1981 gctgcataag ccagggaagt aagcaattta catcgtcgtc cagccgacga tttccagttc
     2041 tgtgccattg ctgatggtag cgaacgattg ggccgcgctg ccattcatgg agccaaaaaa
     2101 caggttgtag gtggtgttct gccccggcgt gtagctttcc agcgcataca acgagtcgtc
     2161 gcgcgagacg ttcgaccaga agtattgcga atacctgttt aagcccaccg tcccctggtt
     2221 aaccgaggta agctgaatgg cgttgtttgt gccatcaacg gcaatcttgt acaggccggt
     2281 ggtggtagct gccaggataa aggcatcgct tttctcgaag acgcgcacgt tggtgatggc
     2341 ctgcgttgaa ctgctaaaca ccgtcgttgg ggccgcgctg ccggttgttt gctgcgcttc
     2401 gaccgtactg gagctacttg aagaagtgga attacactgg ttgatataca cggtcttgcc
     2461 gtcgaagctg ctgtcgaagt cccagcagac attttgctgc gttcccaccg tcgccagcag
     2521 ttgcaggtca ctgctctgct ggttcgcgcc tttgttcgta tccaggagat agacgttttg
     2581 ctggggcgag gcggaattgg gatagtagcc ggtcagcagc acgcgcgagt agtcgagcca
     2641 ggtacgcggc aggta
//
LOCUS       DASZAK010000922         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_698774, whole genome shotgun sequence.
ACCESSION   DASZAK010000922 DASZAK010000000
VERSION     DASZAK010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698774"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="VGT44_16155"
     CDS             <1..321
                     /locus_tag="VGT44_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2582390.1"
                     /translation="RLLDPWQADLRKLAELSNVVCKISGMVTEADHAGWQPADLEPFI
                     TVVLDAFGEERVLFGGDWPVILLASSYQRWVETLTALTAHLPVSARRKLWAENAQRFY
                     RIGK"
     gene            complement(333..1679)
                     /locus_tag="VGT44_16160"
     CDS             complement(333..1679)
                     /locus_tag="VGT44_16160"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582391.1"
                     /translation="MPSPEIKTPSSQRTPPTVRLGLRANWQQFTLLVIINAFVGSMVG
                     LERSIVPLLGQEVFGLASTTAVLSFIVSFGVVKALANLFAGRLSDRIGRKGVLVVGWL
                     FGLPVPFLIIFAPSWSWVVFANVLLGINQGLCWSTTVIMKIDLVGPARRGLAMGINEA
                     AGYLAVSLAAIAASYLAATYALRPQPFLLGIGFALAGLTLSALFVRESRGHARHEAAL
                     QASSPGQSPVTAKQTRPSIQQAPAPSFKDIFVLTSWKNKTLFSACQAGLVNNLNDGLA
                     WGLFPLFFAAHRLNLAQIGLLAGIYPGVWGISQLLTGALSDRIGRKGMIAGGMMLQGL
                     AIGLIALTGGFAWWALDLILLGLGTALVYPTLLAAISDVVQPGWRASAVGVYRLWRDS
                     GYAIGALLAGLLADLFGVSWSIAVIGGVTFLSGIVVQAVMRETLPSRRATAPSVVS"
     gene            complement(1666..2769)
                     /locus_tag="VGT44_16165"
     CDS             complement(1666..2769)
                     /locus_tag="VGT44_16165"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2582392.1"
                     /translation="MLTIDVETLRTWLAEGRGVTILDVRPTPERVEWAIPGSIHVDAY
                     DALQAHEPGALAQVDLPKDVPVVTVCGAGKTSLIAAELLQARGNQVYSLDGGMKAWSL
                     AWNTAEVPVAGDSVRVIQVRRTGKGCLSYLIGAGEEAIVIDASLDPRIYLDLAAQYGW
                     QITSVFDTHIHADHLSRSNQLARQSGAMLLLPEQQRVSFPFTAMRDGDTLTTPTLTLT
                     ALHTPGHTDESTCYLLNQHLLFTGDTLFTTSIGRPDLEADEEQARVRASSLYRSLHKL
                     LALPSETLVLPGHTSTPVPFDGRPIAATLGEIDEAVEIIHAPRDLFLQRITQHLPPTP
                     PNYHRIVRLNEMGKLPESDVTELEAGANRCAIS"
     gene            complement(2763..3452)
                     /locus_tag="VGT44_16170"
     CDS             complement(2763..3452)
                     /locus_tag="VGT44_16170"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2582393.1"
                     /translation="MEKLEKRQFKNVLYEQFARIGKALSNGHRLELLEVLAQGEHSVE
                     TLAQETGLSIANASQHLQVLRSAQLVEVRREGVYQYYRLADEQVFTLWQAMRSVGERR
                     LAEIDRIVQTYLRDRSLLQPISATELLQRLMEGNIILLDVRPAEEYAAGHLPNALSFP
                     IAKLEARLPELSQDKEIVAYCRGPYCVFADEAVTVLRTHGYQARRLEQGLPDWQALGM
                     PVVNGKGVSRC"
BASE COUNT          736 a         1029 c         1052 g          661 t
ORIGIN      
        1 cgtttgctcg acccctggca ggcagacctg cgcaaactcg ccgagctgtc aaacgttgtc
       61 tgtaaaatca gcggcatggt gacagaggcc gaccacgcgg gctggcagcc cgccgacctg
      121 gaaccgttca tcactgtcgt cctggatgcc tttggggaag agcgtgttct tttcggcggt
      181 gactggcctg ttatcttgct cgcatcatcc taccagcgct gggtcgagac gttgactgcc
      241 ctcacagcgc acttgccggt ctcggccagg cgcaagctgt gggcagaaaa tgcgcagcgc
      301 ttctatcgaa ttgggaaata acgcggctgc catcaactca ctacggaggg agcggtagcc
      361 cggcgcgagg gaagcgtttc ccgcatgacc gcctgaacga caatcccgga gagaaaggtc
      421 acgcccccga tgacagcaat ggaccatgag acaccgaaaa gatcggccag cagccctgcc
      481 aacagcgcac cgatggcgta cccactatcg cgccagagcc ggtagacgcc caccgccgag
      541 gctcgccaac ccggttgaac gacatcagaa atggcggcaa gcagcgttgg atagaccagg
      601 gcggttccca gaccaagcag gatcagatcg agcgcccacc aggcgaaacc acccgtcagg
      661 gcgattagac cgatggcgag accctgaagc atcatgcctc ccgcgatcat gcccttgcgt
      721 ccaatgcgat cggatagcgc gccggtcagc aactgcgata ttccccacac acccgggtag
      781 atacccgcca gcagaccaat ctgcgcgaga ttcaagcgat gcgcggcaaa aaagaggggg
      841 aacaggcccc aggccagtcc atcgttcagg ttgttgacca atccggcctg gcaggctgaa
      901 aaaagcgttt tgttcttcca cgaagtcaac acgaagatgt ctttgaagga tggagccggt
      961 gcttgttgaa tggatggccg ggtctgtttt gccgtaactg gcgactgtcc aggagaggat
     1021 gcctgtagcg ccgcttcgtg acgcgcgtgt ccgcgtgatt cgcgcacgaa cagcgccgac
     1081 agcgtcaatc cggccagcgc aaatccaata ccaagcagga atggttgggg ccgcagcgca
     1141 taggtcgcgg ccaggtagct ggcggcgatg gcagccagag aaaccgcaag ataaccggct
     1201 gcttcattga tgcccattgc cagcccgcgc cgggcgggtc ccaccaggtc gatcttcatg
     1261 atgacggtgg tggaccagca caatccctgg ttgatgccaa gcaaaacatt ggcaaagact
     1321 acccaactcc aggacggcgc aaagataatc aagaacggta cgggaaggcc gaagagccag
     1381 ccgaccacca gtacgccctt tcgtccgata cggtcgctca gtctccccgc gaagagattt
     1441 gccagggctt tgacgacgcc aaaactaacg atgaaggaga gaaccgccgt cgtcgaagcc
     1501 aggccgaaca cctcttgacc aagcagtggc acgatgctgc gctccagtcc caccatactg
     1561 ccgacgaagg cattgatgat gaccagcagt gtgaactgct gccagttggc gcgcaggccg
     1621 agtcgaacag tcggcggcgt cctttgagag gaaggtgtct tgatctcagg agatggcaca
     1681 gcggtttgct cctgcttcca attcggttac gtcactttcg ggcaacttcc ccatctcgtt
     1741 caacctgacg atgcggtggt agttgggcgg tgttggcggc aggtgttgcg tgatgcgctg
     1801 gagaaacaga tcacgcggcg cgtggatgat ctcgaccgcc tcgtcgatct cgccaagcgt
     1861 tgcagcaatg ggacgaccat caaatggcac cggcgtgctg gtatgtcccg gcagcaccag
     1921 cgtctcggat gggagcgcga gcagcttgtg caggctgcga tagagcgagg aagcgcgtac
     1981 ccgcgcctgc tcctcgtctg cttcaaggtc cggtcgtccg atgctcgttg tgaaaagggt
     2041 gtcgcctgtg aatagaaggt gttggttgag caggtagcag gtgctttcat cagtatggcc
     2101 cggtgtgtga agggcggtca gcgtcaaggt aggcgtcgtg agagtgtcgc catcgcgcat
     2161 ggcggtaaag ggaaaggaga cgcgctgttg ctcaggcagt aggagcatcg cgccgctctg
     2221 cctggcaagc tggtttgaac gcgagagatg atcggcatga atgtgcgtat caaagacgct
     2281 ggtgatctgc caaccgtact gtgctgccag gtcgagatag atgcgcgggt cgagtgatgc
     2341 atcgatcacg atggcctcct cgcctgcgcc aatcaggtac gagaggcatc ctttgccggt
     2401 gcgccgcacc tgaattactc gcacagagtc gccagcaacc gggacttccg ccgtattcca
     2461 tgccagactc cacgccttca tacctccatc gagggagtag acctggttgc ctcgcgcctg
     2521 gagcaactcg gcggcgatca agctggtctt tcccgcccca cacaccgtca cgaccgggac
     2581 atcctttgga agatccacct gggcaagcgc gccgggttcg tgcgcttgca gggcatcata
     2641 tgcatcgacg tggatgctcc ctgggatggc ccactccacg cgctcaggcg ttggacggac
     2701 atccaggatg gtcacgcctc gcccctccgc cagccaggtt ctcaaggttt caacgtcaat
     2761 cgttagcatc gcgatacccc ttttccgttt acgaccggca tccccaaagc ttgccaatcg
     2821 ggtaatcctt gttcaagccg acgcgcctgg tagccatgag tgcgcaggac ggtgacggcc
     2881 tcgtccgcga agacgcagta ggggccgcgg caataggcca cgatctcttt gtcctgcgac
     2941 agttccggga ggcgagcttc cagtttggcg atgggaaaag agagcgcatt gggcaggtgt
     3001 ccagcagcgt attcctcggc gggacgcaca tcaaggagga tgatattgcc ctccatcaag
     3061 cgctggagca gttccgtcgc gctgatgggc tgcaagagcg aacgatcacg caggtaggtc
     3121 tgcacaatgc gatcaatttc agcgaggcgg cgctctccta cgctccgcat ggcttgccac
     3181 aaggtaaaca cttgttcgtc cgccaggcga tagtactggt agacgccctc gcggcgcacc
     3241 tcgaccagtt gagcggagcg cagcacttgc aggtgctgcg aggcgttggc aatcgacagg
     3301 ccggtttctt gtgcgagcgt ctcaacgcta tgttcgccct gggccagcac ttcgaggagt
     3361 tcgaggcgat ggccgttcga cagcgcctta ccgatgcgcg caaactgttc gtacaacaca
     3421 tttttaaatt gtcgtttctc tagcttctcc attgttcaca ccctatgatc cttcaacc
//
LOCUS       DASZAK010000923         8168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_698790, whole genome shotgun sequence.
ACCESSION   DASZAK010000923 DASZAK010000000
VERSION     DASZAK010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698790"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VGT44_16175"
     CDS             <1..422
                     /locus_tag="VGT44_16175"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582394.1"
                     /translation="RLEEDALRAAGLSPQKIRYMRDLTERITDGRLDLARLSELEDEA
                     VIEELVKVKGIGRWTAEMMLIFSLGRPDVLPVDDLGFVEGVRDAYGLEKRPTPKEMRE
                     RGEPWRPYRTFATWYMWGMRRLAMNIAPRERTRIVSL"
     gene            440..1864
                     /locus_tag="VGT44_16180"
     CDS             440..1864
                     /locus_tag="VGT44_16180"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV2582395.1"
                     /translation="MDTLDNYYAILGVPPDADSDTLKRAYRQLARRYHPDLAGPEGAL
                     EMKRINRAYDVLSDPQKRLNYDTIIGGAIDLRKGGITRPRPVPRKFDPSDDIEFAGLS
                     IFSTKGPLRAGPKLHSQLGVISALSSVQYEAGLCIAAGSLDGKGLIWHPGAEIEPGKP
                     QARESQHTMVSFAADGAFAVESLRELRFSASGGLVSGWGRSSLHVWQSHDGALLWSYG
                     LSERAVSAHYSIDVALKETNGAREVWMALPLLRDDPRAPRSLGVRGTDVVRHVIGDTP
                     SGLSSPLVCAEDEIEKRQFWAIRLRALAQDTRALLTLSCAHVPDEQDEMVVIRRWDTQ
                     ARSKTRFREVVQPQITGSLIAGLCANCTPPYAVTPDMRALAFVHANKSIRIHDTTSGL
                     FSELPSGTMGASSRLAISPDAQWVAVAREDSEVNEGVIDLWSVPRSEVAQKLYHPWQI
                     SALHFAPGRLLVSLTDGTIQVWQL"
     gene            2013..2771
                     /locus_tag="VGT44_16185"
     CDS             2013..2771
                     /locus_tag="VGT44_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2582396.1"
                     /translation="MRISVVVPVYNEEETVAQVLESLAQTPLDLEVVVVDDASTDRTW
                     EILQELRSQEPFTTYRFVRHDRNQGKGAGLRTGFGLVSGDLVTVQDADMEYDPQDLPA
                     LLRKWEAARDAGMARVAIYGCRDLSPQKFTTRWGNRFLTTFTNLLYGCHIHDMETCYK
                     MFPGPLARALPMEGRRFEIEPEITACIVQAGYTILEVPISYTPRKAKKLSPFKDGWPA
                     LAMLLRRRFFKRFQYVEPASTNVEAKQATTSIPK"
     gene            complement(2816..6058)
                     /locus_tag="VGT44_16190"
     CDS             complement(2816..6058)
                     /locus_tag="VGT44_16190"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2582397.1"
                     /translation="MPGLREEGHTSMLKENTPGEVQELRIYLLGGFRVMVGERAITEW
                     RLRKAKSLVKMLALAPDHRYHREQLLDQLWPDLEPEAALNNLHRVVHTARSILEPQLA
                     SLRNSAYLHAQDDVLSLSPSLPVWIDVEQFELARSAIREAQEPALYQSALALYSGDLL
                     PEDRYEDWTIRRREQLRQSYLALLSDLGRVYIARQEHHAAIETLQKLVAEEPTLEEAH
                     LELIRYSLETGQRYQALRQYQQLRDALRRDLDAEPSIPAQRLYRDIQAGRIAQEQEQA
                     HPAAPVLDGVQELSISHQDVEKDGQAQEQQEQADRPTNLPHALTSFVGRGDEMGEIRR
                     LLTQTRLITLTGVGGSGKTRLAIHTAATLAEMYSDGVWLVELAALTDPSLVPQEVATA
                     LDVRETAARSLVEGLIEALRSKRILLLLDNCEHLVDACAELAETLLQACRHLRILATS
                     RQALGVMGEVAWQTPSLAAPHPEHLPPFEHLPGYDAVRLFLERVATSRPGVALTPANA
                     SSVVQICHRLDGIPLALELAAVRVKALSVEQIAARLDNTLALLVTGNRAALPRQQTLQ
                     ATIDWSFALLNEEEQTLFRRLSVFAGWWSLEAAEGICAGENVAAARVLGLLDQLVNKS
                     LVVAEEHDGRMRYRLLEVLRQYGAEKLASAGETSLVRERHARWYLAMDEGGKARLEGP
                     EQGEWMEVLEGAHDNLRVALRWALDKDDAEYGLRLGSAVWRFWQVRGYLSEGRKWFGA
                     LLAMHQGKMESLRMQRAQALLGAGVLAMDQGDFQYARGLYEESLALYRQLQEKAGITR
                     ALNNLGIVVENLGEFQQAVLLYEESLELARESGDQDVLARALNNLGVVTYHMGDYQRA
                     EELYQESLAIMRALDNKLGVIVALNNVSEAACRQGDYQRAVVTYKEGLAQAQRLGFKT
                     AIAFFLGGLAETAAGLRQTARSVRLWGASDTLRTEIGAPLEPADLERYQQAVTHIRAM
                     LGEEAFGELWEAGRAMPLDQAVLYALSEEPSGAEMPSPAKSSALLSQREWQIAKLIAE
                     GRTNREIADTLAIAQRTVDTHVGHMLSKLGFSSRAQISGWIVEEQQLRPA"
     gene            6293..8011
                     /locus_tag="VGT44_16195"
     CDS             6293..8011
                     /locus_tag="VGT44_16195"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2582398.1"
                     /translation="MSGSIKHYMRLILFPTLLALLLASSLSLLVASTHVTRAQALAQN
                     SISQPVQLDSPAIVRIISVVIGRVICYGCASDGSNVVSPQKNYFGWASSGSGVFVSPD
                     GYILTADHVVDHTTSNPGDVDYLLNAAANDIASRYNTTPANALQILQDQYNQGNLGIT
                     IQVQSQTVFLSTAYTGQLQNTAQMISYPLTRVVASSPVDKQDTAIIQIEAHDMPFLSL
                     AQNSTINIGDSVTAVGYPGDADCVVNNCDFTALLPPIQSDANTLSSLLDLSASTGQVT
                     SKKSWPDGTPVYVVGGEIASAGSSGGPIIDQQGNIIGFVDAAPSSNRITFVVPSSVIQ
                     SYMQQSGISSPQHGKFMSLWTKAINDYTGSVACHWTNATRELTALHNDYPQFGAALSL
                     MQNAQSNVTPTECPAPSQSFNIVPLIGGIAAVALLGTLLVIFFLRRRRQPGIPAPVVM
                     PSAINTPVPVSVSSPALPQSHPQYAPYPPQGSTGYGYGSQVWQNTYNSQGQTYPASQG
                     LPAQAAPVTPVMYPVQTPTPAQAFPPVQSPTPFRQCTSGHIIYEREARFCPDCGSTVH
                     EVPVRQ"
BASE COUNT         1676 a         2561 c         2290 g         1641 t
ORIGIN      
        1 tgcggttgga ggaagacgcg ctgcgggcag cgggactttc gccgcagaag atacgttaca
       61 tgcgcgattt gacggagcgc atcaccgatg gtcggctcga cctggcgcgg ttgagcgagt
      121 tggaagacga ggccgtcatc gaagaactgg tcaaggtcaa gggtattggc cgctggaccg
      181 cggagatgat gctgatcttc tcattgggaa gacccgatgt gctacccgtc gatgacctgg
      241 gcttcgtcga aggtgtgcgc gacgcctacg gcctggaaaa gcgcccgaca ccgaaggaga
      301 tgcgcgagcg cggcgagccg tggcgaccat accggacgtt tgcgacgtgg tatatgtggg
      361 ggatgcgcag gctggcaatg aatattgcgc cacgcgagcg gacgaggatt gtttcgttgt
      421 agatgatact atgaggagca tggacacact cgacaactac tacgccattc ttggcgttcc
      481 gccagatgcc gatagcgaca cgctcaaacg cgcctaccgc caactcgctc ggcgctacca
      541 tcccgacctg gcggggccgg agggcgcgct ggagatgaaa cgcatcaacc gcgcctacga
      601 tgtgctgagt gacccgcaga agcgtttgaa ctacgatacc atcatcggcg gcgcgatcga
      661 cctgcgcaaa gggggtatca cgcggccacg tcctgttccg cgcaaatttg atccctcgga
      721 cgacatcgag ttcgcgggcc tgagcatttt cagcaccaag ggcccattac gtgccgggcc
      781 taagctccat tcgcaactcg gcgtgatctc tgcgttgagc agtgttcaat acgaagcggg
      841 actatgcatc gcagccggtt cgctggatgg caaaggattg atctggcacc ccggcgcgga
      901 aattgagccg ggcaagccgc aggcaaggga gtcgcaacac acgatggtca gcttcgcggc
      961 ggacggcgct ttcgcagtgg aatcgctgcg cgagctgcgc ttctccgcga gcggcggact
     1021 cgtgtccggg tggggcagat caagcctgca tgtctggcag tcgcacgatg gcgcgctgtt
     1081 gtggagttat ggactgagcg agcgtgccgt atccgcgcac tattcgatag acgttgcgct
     1141 caaagagacc aacggcgcgc gagaagtgtg gatggcactg ccgctcttgc gtgatgatcc
     1201 acgcgcgccg cgttcgctgg gagtgcgtgg tacggatgta gtccgacatg tgatagggga
     1261 caccccctcc ggtctgagca gcccgctggt atgcgcggaa gacgagatcg aaaagcgtca
     1321 attctgggct attcgcctgc gagcgctggc gcaggacacg cgcgcgctgt tgacgttatc
     1381 ctgcgcccac gtgccagacg agcaggatga gatggtcgtc atacgccgct gggatacaca
     1441 ggcgcgcagc aaaacgcgct ttcgcgaagt cgttcagccg cagatcaccg gctcgctgat
     1501 agcgggactg tgcgcgaatt gcacaccgcc ctacgcggtc acaccagata tgcgcgcgct
     1561 ggcattcgtc cacgcaaaca agtctatacg cattcacgat accacaagcg gcctcttcag
     1621 cgaactacca tcaggcacga tgggggccag ttcgcgcctg gctatctcgc cggatgcgca
     1681 atgggtcgcg gtggcgcgtg aagacagcga ggtcaatgag ggtgtgatcg acctctggtc
     1741 cgtgcctcgc agcgaggttg cacagaagct ttaccacccc tggcagatca gcgctctgca
     1801 ttttgctcct ggacggctgc tcgtctcgtt aaccgacggc accattcaag tatggcagtt
     1861 ataatgcggc cagagtcgta gggacgctat tcatcatcat ttattaccat aatgtgttgc
     1921 tattttagtc gccgttcgtt cgtgtataat agcaggatgt aggaatcagt tccattggag
     1981 cagcactccc ggagaagtga ggtagagcta tcatgcgtat ttcggttgtc gtacccgtct
     2041 ataacgaaga agagaccgtg gctcaagtgc ttgagtcgct ggcacagacg ccacttgacc
     2101 ttgaagtcgt cgttgttgac gatgcctcaa ccgaccgtac ctgggagatt ttgcaggaat
     2161 tacgcagtca agagccgttc actacctacc gcttcgtgcg tcacgatcgt aaccagggca
     2221 aaggcgcagg actgcgcacc ggcttcggac tggtcagcgg cgacctggtg acggtgcagg
     2281 atgccgatat ggagtatgac ccgcaggacc tgcccgcgct gctgcgcaag tgggaagccg
     2341 ccagggacgc tggtatggcc agggtggcta tctatggatg ccgcgacctg tctccacaaa
     2401 aatttacgac gcgctggggc aaccgcttcc tgaccacctt caccaacctg ctctatggct
     2461 gccacatcca cgatatggaa acctgctaca aaatgttccc cggcccgctg gcccgcgcgc
     2521 tgccaatgga aggccgccgt ttcgagatcg agccggaaat caccgcgtgt atcgtgcagg
     2581 caggctacac catcttagaa gtccccatca gctatacacc gcgcaaggcc aaaaagctct
     2641 cgcccttcaa agacggctgg cccgcgctgg ctatgctgct gcgccgccgc ttcttcaaac
     2701 gctttcagta cgtagagcct gcatcgacca acgtcgaagc aaagcaggcg acgacgagca
     2761 taccgaagta accatatcct cccccacaat ctgttaataa atccagcgct gaagatcagg
     2821 ccgggcgcaa ttgttgctct tcaacgatcc acccgctgat ctgcgcgcgc gagctaaaac
     2881 caagcttgga gagcatgtgc ccgacgtgcg tatcgacggt gcgctgcgca attgccagcg
     2941 tatccgcgat ctcgcgattg gtacgaccct cggcaattag ctttgcgatc tgccattcac
     3001 gctggctgag caaggccgac gacttcgcag gcgagggcat ctccgcgccg gatggctctt
     3061 cagacagggc atacagcacg gcctgatcga gcggcatcgc ccggcctgcc tcccatagct
     3121 ccccgaaagc ctcttcgccc agcatggcgc gtatatgtgt cacagcctgc tggtagcgct
     3181 ccaggtcggc aggttccaac ggggcgccaa tttctgtacg cagagtatcc gacgcgcccc
     3241 acagccgcac ggatcgagcg gtttgtctga gtcccgccgc tgtctccgcc agtcctccca
     3301 ggaagaaagc gatggccgtc ttaaatccca gccgttgagc ctgagccagg ccctccttgt
     3361 aggtcactac cgcgcgctga taatcgccct ggcgacaggc cgcctcactg acattgttca
     3421 aagcgacgat cacgcccagc ttattatcca gcgcgcgcat gatcgccagg ctctcctggt
     3481 agagttcttc tgctcgctga taatctccca tgtggtaggt cacaactccc aggttattga
     3541 gagcgcgagc cagcacatct tgatcgcccg actcgcgggc aagttcaaga ctttcttcat
     3601 acaacagcac ggcctgctga aattctccca ggttctccac cacaataccg agattgttca
     3661 gcgcccgcgt gatgcccgct ttctcctgca actgccggta gagcgccagg ctctcctcgt
     3721 acagcccgcg cgcgtactga aagtcgccct gatccattgc caacactccc gcgccaagca
     3781 gcgcctgcgc ccgctgcatg cgcaagctct ccattttgcc ctgatgcatg gccagcagcg
     3841 ccccaaacca tttgcgccct tcgctcaaat agccgcgcac ctgccagaag cgccacaccg
     3901 cgctgcccag acgtaggcca tattccgcgt catccttgtc cagcgcccag cgcagcgcga
     3961 cgcgcaggtt gtcgtgcgcg ccttccagca cttccatcca ctccccttgc tcaggacctt
     4021 cgagcctggc cttcccgcct tcatccattg ccaggtacca gcgcgcgtgg cgttcgcgca
     4081 caagggatgt ctccccggcg cttgccagtt tctccgcgcc atactgtcgc aaaacctcca
     4141 ataagcggta gcgcatccgg ccatcatgtt cttccgcaac aaccagggat ttattgacga
     4201 gttgatccaa caggcccagc acgcgcgctg ctgcgacgtt ctcccccgcg catataccct
     4261 ctgccgcctc aagcgaccac cagccggcga agacagataa gcgccggaaa agggtctgct
     4321 cctcctcatt gagcagtgca aagctccaat cgatggtcgc ctggagcgtt tgctgccgtg
     4381 gcagtgccgc gcgattccct gtgaccagca acgcgagcgt gttatccagg cgcgcggcga
     4441 tttgttctac cgagagagcc ttaacgcgaa cggcagccag ttccagcgcg agtggaatac
     4501 catccaatcg atggcaaatc tgcaccaccg aggatgcatt ggccggtgtg agcgcgacac
     4561 ccggacggct tgttgcaaca cgttccagga agagccgcac cgcgtcataa ccggggagat
     4621 gctcgaatgg gggtaaatgt tcgggatgag gcgcggccaa cgacggcgtc tgccaggcca
     4681 cctcacccat gacgccaagc gcctgccgac tggtcgcgag aatgcgcagg tgacgacacg
     4741 cctgcaacaa ggtctccgcc agttccgcgc aggcatccac caggtgttcg cagttatcga
     4801 gcagcagcaa gatgcgtttt gagcgcagcg cctcgatcag cccttccacg agcgacctcg
     4861 cagccgtttc acgcacatcc agtgcggttg ccacctcctg tggcacaagc gacgggtctg
     4921 taagagccgc caattccacc agccagacgc cgtcgctgta catctccgca agcgtagccg
     4981 ccgtgtgtat cgccaggcgc gttttcccgc ttccgcccac cccggtcagc gtaatgaggc
     5041 gtgtctgcgt cagcaaacgg cggatttcac ccatctcatc accccgcccg acgaaactgg
     5101 tcagcgcgtg cggcaaattg gttggtcggt ctgcctgctc ttgctgctct tgcgcctggc
     5161 catccttttc tacgtcctga tgcgaaatgg ataactcctg taccccatcc aacacaggag
     5221 ccgccgggtg cgcctgttcc tgctcctgcg cgatgcgacc ggcctgaata tcccggtaga
     5281 gacgctgcgc cggaatcgat ggttccgcat ccaggtcgcg gcgcagggca tcgcgcaact
     5341 gctggtactg gcgcagggcc tggtagcgct ggcccgtttc aagcgagtac cgtattaact
     5401 ccaggtgagc ttcctctagc gtcggctctt ccgctaccag tttttgcagc gtctcaatcg
     5461 cggcatgatg ctcctggcgc gctatgtaga cgcgccccag gtcgcttaac aaagccaggt
     5521 aactctggcg cagttgctcc cgccgcctga tcgtccagtc ctcatagcgg tcttccggca
     5581 gcaggtcgcc gctatacaac gccagcgcgg attgatagag cgcgggttcc tgtgcctcgc
     5641 ggattgcgct tctggccagt tcgaactgtt ccacgtcaat ccacacgggg agcgatgggg
     5701 acaggcttaa cacatcatcc tgcgcgtgta agtaggccga gttgcgcagg gatgcgagct
     5761 ggggttctag tatagagcgc gctgtgtgaa ccacacgatg cagattattc agcgcggcct
     5821 ccggctcaag gtctggccag agctggtcga gcagctgctc gcggtgatag cgatgatcgg
     5881 gcgcgagcgc cagcatcttc accaggcttt tcgctttgcg cagccgccac tccgtgatag
     5941 cgcgctcacc gaccatcacg cgaaacccac ccagcagata aatacgcagt tcctggactt
     6001 cacccggtgt gttctctttg agcatcgacg tgtgtccttc ctctcgcaag ccaggcaaac
     6061 aggccttcca tacaaattgc gtctctttaa ttattggata ggatgccggg caggtcaatg
     6121 cgcccgctca caagcagaat gtgaggtagg tatctgtacg catacgtatc tacaaaaaag
     6181 tcatgcgagg taagtgccag gtaagggacg gttggtatgc tccttgcaga tagtgtatcc
     6241 cgattctgaa atgtatgcca actcggtgaa gtctggagaa aggcaggtcc ctatgtctgg
     6301 atcgatcaaa cattacatgc gactcattct cttccccaca ctgctggcgc tgctactggc
     6361 aagcagcctt tcacttttgg tagcatcgac ccacgtaaca cgcgcgcaag cgctcgcaca
     6421 aaatagcatc agccagccgg tacagttgga tagcccggcg atcgtgcgca tcatctcagt
     6481 cgtgattggg cgtgtgatct gttacggctg tgccagcgat ggcagcaacg tcgtttcacc
     6541 ccagaagaac tacttcggat gggcctccag cggttcgggc gtgttcgtca gccccgatgg
     6601 ctatattctg accgccgatc acgttgtaga ccacaccacc agcaatcctg gcgacgtgga
     6661 ttaccttctt aatgcggcgg cgaatgacat cgcttcacgc tacaatacca caccggcgaa
     6721 cgccttgcag attctgcaag accagtacaa ccaaggcaac ctggggataa ctatccaggt
     6781 gcaaagccag acggtctttt tatcgaccgc ctacacgggg caactgcaga acacggctca
     6841 gatgattagc tatcccctca cacgtgtggt cgccagttcg ccggttgaca agcaggacac
     6901 cgccatcatt cagattgaag cgcatgatat gccctttctc agtcttgccc agaacagcac
     6961 gatcaatatc ggcgattccg tcacagcggt tggctacccc ggtgatgccg actgcgtcgt
     7021 caataactgc gatttcaccg ctctacttcc ccctatccag agtgacgcga acacgctcag
     7081 cagcctgtta gacctctcgg ccagtaccgg ccaggtcacc tccaagaaga gttggccaga
     7141 cggcacgccg gtctacgtcg tcgggggcga gattgcatca gcagggtcga gcggcggccc
     7201 gatcatcgat cagcaaggaa acatcatcgg ttttgttgat gccgcaccct ccagcaatcg
     7261 catcacgttc gttgttccca gcagcgtcat ccagagctac atgcagcaat ccggcatttc
     7321 cagcccgcag catggtaaat ttatgagctt atggacgaag gccatcaacg attacactgg
     7381 ctcagttgca tgtcattgga ccaatgccac cagagagttg actgcgctgc acaacgacta
     7441 cccacagttt ggcgctgcgc tctcactcat gcaaaacgcg caatcgaatg tcacgccgac
     7501 cgagtgtccc gcgccatcac agtcgttcaa tatcgtacca ctgattggtg ggatcgccgc
     7561 agtcgccctg ctcggcacac tgctggtcat cttcttccta cgccgccgca ggcaaccggg
     7621 cataccagcg ccagtggtga tgccgtcggc catcaatacg cccgtaccag tctcggtttc
     7681 atcgcctgct cttccgcaat cgcacccgca gtacgcaccc tatccgccgc aaggttctac
     7741 tggctacggt tacggttcgc aggtgtggca gaacacctac aacagccagg gccagaccta
     7801 cccggcttcc caggggctgc cagcgcaggc tgctcccgtc acgccggtca tgtatcccgt
     7861 ccaaacaccg acgcccgctc aggcatttcc acccgttcaa tcgccgacgc ccttccgcca
     7921 atgtaccagt ggacatatca tctatgaacg agaggcgcgc ttctgccccg attgcggatc
     7981 gaccgtacac gaggtgccag ttcgtcagta gtccgtaagg cgatcccata ggtgttcact
     8041 taatgtccag gacgatatcc tgggtcgccc ttaccaatct ggaatgtcat cctgagcgca
     8101 gcgaaggatc ttggtgccga caagtcagac ccttcgctgc gctcagggtg acactcgacg
     8161 gcaggccg
//
LOCUS       DASZAK010000924         6253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_165920, whole genome shotgun sequence.
ACCESSION   DASZAK010000924 DASZAK010000000
VERSION     DASZAK010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165920"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="VGT44_16200"
     CDS             <1..711
                     /locus_tag="VGT44_16200"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV2582399.1"
                     /translation="LGYLAVANVLLGIFNLVPAFPLDGGRVLRSIIWKISGNVRTATR
                     AATFVGQAIAYLLILAGFYLIFGGDILDGIWFGLIGWFLLSGAQSANSQAMLESMFKG
                     VTVAEVMNAAPTTVPANISLQQLVDELLLPHGWRSAFVMQVDRFVGLITLGDIRHVAR
                     DEWATTPVGLAMIPREKLHAVSPQQSLNEVLPLMVDRDVNQLPVVEDDRLVGVLSRED
                     IVRFLEVRRSLGLETGGR"
     gene            749..1192
                     /locus_tag="VGT44_16205"
     CDS             749..1192
                     /locus_tag="VGT44_16205"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEV2582400.1"
                     /translation="MEIGPLEYVVLGFEDGQFASVVLPKLNEIQESGLIRVVDLLFVG
                     KAADGAVSMQEVSELGEDEQAAYAGLAEDVAGLLTAEDIDRLASEIPPGTWAVVALFE
                     HTWTLQLADAVRRAGGALFAGGMVAPDAIGKVSAELAAAKEEHHA"
     gene            1185..1571
                     /locus_tag="VGT44_16210"
     CDS             1185..1571
                     /locus_tag="VGT44_16210"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEV2582401.1"
                     /translation="MPRGGFGGGFGRGFGRGFGMRRFGAPLVMPMGGYGMGGFGSPLL
                     TSLLAGGVGYALGSNSAQQGAQQPPPYQQPYPYPYPYQAPPSAPQGAGADGGKLAQLQ
                     LLGELHESGTLTDDEFERQKQRILGS"
     gene            1797..3338
                     /locus_tag="VGT44_16215"
     CDS             1797..3338
                     /locus_tag="VGT44_16215"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2582402.1"
                     /translation="MSTATQPRTYVNPGNPDSVVAVKPRYDNFIGGRWVPPVKGEYTT
                     VLSPATGKSFTQVPRSTPEDVEVALDAAHAAKDAWGETSPAERSRILNKVADAMEANL
                     EMLAVAESWGNGKPVRETLAADLPLAIDHFRYFAGAIRAEEGRISEIDKNTVAYHFQE
                     PLGVVGQIIPFNFPLLMAAWKIAPALAAGNCTVIKPASPTPWSILKLAEVIGDIVPPG
                     VLNIVNGPGAEIGKALATSKRIAKIAFTGETTTGRMIMQYAAQNIIPSTTELGGKSPN
                     IFFADIMNADDAFFDKAIEGLVLYAFNKGEVCTCPSRALIQESIYDAFMERVLPRIAA
                     IRQGNPLALDTQIGAQVSRAQLEKIESYVKIGLDEGAKVLIGGERTHLNGELAGGYYY
                     QPTVLKGQNNMRVFQEEIFGPVLAVTTFKDEREALALANDTLYGLGAGVWTRDMARAY
                     RMGRGIKAGRVWTNCYHLYPAHAAFGGYKVSGVGRETHLMMLEHYSQTKCLLVSYDPK
                     PLGFF"
     gene            3349..3687
                     /locus_tag="VGT44_16220"
     CDS             3349..3687
                     /locus_tag="VGT44_16220"
                     /inference="COORDINATES: protein motif:HMM:NF017426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HEV2582403.1"
                     /translation="MSESVKGTEAARELIARLKTRYGSLMFMQSGGCCDGSSPVCLSE
                     GDLLLGPGDLLIGEIEGCPFYIDREQYERWREPEFLIDVSPGGGDTFSLEGPEGLHFV
                     ARTESKPGAQ"
     gene            3758..4918
                     /locus_tag="VGT44_16225"
     CDS             3758..4918
                     /locus_tag="VGT44_16225"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEV2582404.1"
                     /translation="MRIGIPKEVQAGETRVALIPSLVPLLIKDRHEVLVEAGAGVGAY
                     FSDTQYLQAGASLVEGVPALYEQVDVIFKVQPPQMHPAAHLPEAEMMHEGSAYIGLLS
                     PFTHLEVMETFARRKITAFAMDFVPRLTRAQSMDALSAMATVAGYKAVLLATEHLGKM
                     FPLLMTAAGTITPATVLVLGAGVAGLQAIATAKRLGARVEAFDARPAVREQIQSLGAT
                     FIEMELPQDAETAGGYAKEQSAEFLKREQEVICARFPRVDVVISTAQVFGKRAPLLIT
                     TEMVKSLRPGSVIVDLAAEQGGNCELTQAGQIIKYEGITVVGVTNVPAMLPIDASQLY
                     ARNVVNLFRYLYPASGVQPDSADELVKGVCVTRSGAIVHELLQSSIREGVST"
     gene            4915..5211
                     /locus_tag="VGT44_16230"
     CDS             4915..5211
                     /locus_tag="VGT44_16230"
                     /inference="COORDINATES: protein motif:HMM:NF024179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEV2582405.1"
                     /translation="MNSLLFESLYILVLAGFLGFEVIRKVPPLLHTPLMSTTNAISGI
                     SLVGSLVAAGSGQSTVSTVLGTLAVTAATINVVGGFLITDRMLKMFRRKESKPQ"
     gene            5208..>6253
                     /locus_tag="VGT44_16235"
     CDS             5208..>6253
                     /locus_tag="VGT44_16235"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEV2582406.1"
                     /translation="MSDVLLAAAYLAAAILFVLGLRGLNSPQTARRGILLAEIGMLLA
                     VVGTLLRSEIIQYQWILVGLAVGSTIGALMAIFMPMTAMPQRIAISHSFGALAATLVG
                     IVEYYEHGLTIDHGTMAALGFEVLFGSLTITGSLMAFGKLQDLVKSAPITYRFQNVSN
                     ILLFCATVGVFVFLIVRPGTSALFYGMIAAGLLFGILIVLPIGGADMPVVLSLLNSYA
                     GLASAATGLVISNNVLLIAGALDGTSGFFLSMMMSRAMNRSFTNVLFGAVGAVKAGVQ
                     APGEGEEARTVMRYTPEDAAYILESAQSVIIIPGYGMAVAQAQHAVQELAALLIERGA
                     TVRYAIHPVAGRMPG"
BASE COUNT         1207 a         1802 c         2004 g         1240 t
ORIGIN      
        1 ctgggctatc ttgcggtggc aaacgtgttg ctcgggatat tcaacctcgt tccggctttc
       61 ccgctggatg gagggcgagt gttacgctcg atcatctgga aaatcagcgg caatgtccgg
      121 acggccacgc gcgccgccac gtttgtcggt caagcgattg cctatcttct gatcctggcg
      181 ggcttctacc tgatctttgg cggggatatt ctggatggca tctggtttgg cctgatcggc
      241 tggttcctgc ttagcggggc gcagtccgcc aattcccagg ccatgctcga gtcgatgttc
      301 aagggggtca cggtggcgga ggtgatgaat gccgcaccca cgaccgtccc cgccaacatc
      361 tcgctgcaac agctcgtgga cgaactcctg ttgccgcacg gctggcgcag cgcgtttgtc
      421 atgcaggtcg accggttcgt cggcctcatt acgctgggcg atatccgcca tgtcgcgcgc
      481 gacgagtggg cgacgacccc ggttggtctc gccatgatcc cgcgcgagaa gctgcacgcg
      541 gtttccccgc agcagagctt gaacgaggtc ttgccgctga tggtggatcg agacgtcaac
      601 cagttgcccg tcgtggagga tgaccgcctg gtaggagtgt tgagccggga ggatattgtg
      661 cgtttcctgg aggtcaggcg cagtctggga ttggagacgg gaggcaggtg actgccctgc
      721 catatcgtat acagaaagga gaactaacat ggaaattggc ccattggaat acgtggtact
      781 tggcttcgag gacggacagt tcgcgagcgt cgtactcccg aaactgaacg agatccagga
      841 gtctggcctc atccgagtcg tggacctgct gttcgtcggc aaagcggcag atggcgcggt
      901 ctcgatgcag gaggtcagcg aactgggcga ggacgagcag gcggcctatg cgggcctggc
      961 ggaggatgtc gcagggctgc tgacggcgga ggacatcgac cggctggcca gcgagatccc
     1021 ccctggcacg tgggcggtgg tggcgctctt cgagcatacc tggacgctcc agctggcgga
     1081 cgcggtgcgc agagccggtg gcgcgctctt cgccgggggg atggtcgccc cagacgcaat
     1141 agggaaggtc agcgcggaac tggccgcagc aaaggaggaa catcatgcct agaggaggat
     1201 ttggcggggg tttcggcaga ggatttggtc gcggcttcgg catgaggcgc ttcggcgctc
     1261 cgttagtgat gcctatgggc ggatacggca tgggcgggtt tggctcgccg cttttgacaa
     1321 gtctgctggc aggaggagtg ggctacgcgc tgggcagcaa ctcagcccaa caaggcgcac
     1381 agcagcctcc gccttaccag cagccctatc cgtatcccta tccgtaccag gctccgccat
     1441 ctgctccgca gggagcaggc gcggacggcg gcaagctggc gcagctccaa ctgctgggcg
     1501 aactgcacga atcgggtacg ctgaccgacg acgaattcga gcgtcagaaa cagcgcatcc
     1561 tgggcagcta gccgcaaagc gggtcaatcc tgtttagacc tgaaattggg acgcatttgt
     1621 gagggaaacg caattttttg tgaccgcgct gagaaagcga gctgctatcc ttcattgttg
     1681 aaggcggaag tattggggag ggatcgtcct gtgagggcgg tatttctcag gattggcttc
     1741 cgccacgaaa ctgtgtttac acactaaaat tgaatctcga agagagagga acaactatgt
     1801 ctacagctac acaaccccgg acctatgtca atccaggcaa ccctgacagt gtcgtggcag
     1861 tgaaaccgcg ctacgacaac ttcatcggtg ggcgctgggt accgccggtc aaaggcgagt
     1921 acaccacggt cctcagcccg gcgacaggga agtccttcac ccaggtgccg cgctcgaccc
     1981 cagaagatgt cgaagtcgcg cttgatgctg cccatgccgc gaaggacgcc tggggagaaa
     2041 catcgccggc ggagcggtcg cgcatcttga acaaggtcgc cgacgcgatg gaggcgaacc
     2101 ttgaaatgct ggccgtcgcc gagagttggg gcaacggcaa gcccgtgcgc gagacgctgg
     2161 ccgccgacct gccgctggcg attgaccact tccgctactt cgcgggggcg atccgcgcgg
     2221 aggaggggcg tatctctgaa atcgacaaaa acacggttgc ctaccacttc caggagccgc
     2281 tcggcgtggt cgggcagatc atccccttca atttcccgct cctgatggcc gcctggaaaa
     2341 tcgccccggc gctggccgcc gggaactgca cggtcatcaa gccggccagc ccgacaccct
     2401 ggtcgatcct caagctcgcc gaagtcatcg gggacatcgt cccgcccggc gtgctcaaca
     2461 tcgtcaacgg gccgggagca gagattggca aggcgctggc caccagcaaa cgcatcgcca
     2521 agatcgcttt tacgggtgag acgacgaccg ggcgcatgat tatgcagtac gccgcccaga
     2581 acatcatccc gtccaccacc gagcttggcg gcaagtcgcc caacatcttc ttcgccgaca
     2641 tcatgaacgc ggatgatgcc ttctttgata aggcgatcga agggctggta ctctacgcct
     2701 tcaataaggg cgaggtctgc acctgtccct cgcgggcctt gatccaggag tcgatctacg
     2761 atgctttcat ggagcgcgtt ttgccgcgca tcgccgccat ccggcagggc aacccgctcg
     2821 cgctcgacac gcagatcggc gcgcaggtgt cacgcgccca gctcgagaaa atcgagagct
     2881 atgtcaagat cggcctcgac gagggggcga aagtgctgat cggcggcgag cggacccatc
     2941 ttaatgggga actcgcaggt gggtattact accagcctac cgtgctgaag gggcagaaca
     3001 acatgcgcgt tttccaggag gagatcttcg ggccggtgct ggcggtgacg accttcaagg
     3061 atgagcgcga ggcgctggcg ctggccaacg acaccctcta cggcctgggc gcgggcgtgt
     3121 ggacgcgcga catggccagg gcgtatcgca tgggccgggg catcaaagcg ggccgcgtgt
     3181 ggaccaactg ctaccacctc tacccggcgc acgctgcctt cggtggatac aaggtttcag
     3241 gggttggtcg cgagacccac ctcatgatgc tcgaacacta ttcgcagaca aagtgcctgc
     3301 tggtcagtta cgatcccaag ccgttgggat ttttctagga gtacagtcat gagtgaaagc
     3361 gtaaaaggga cggaggcggc gcgtgagctg attgcgcggc tgaagactcg ctatggctcg
     3421 ctcatgttca tgcaatcggg gggctgctgt gatggcagct ccccggtttg cctgagcgaa
     3481 ggcgacctgc tgcttggacc gggcgacctg ctcatcggag aaatcgaggg atgcccgttc
     3541 tacatcgacc gggagcagta cgagcgctgg cgcgagccag agttcctgat tgacgtctcg
     3601 cccggcggag gcgacacctt ctcgttggaa gggccggagg gattgcactt cgtcgcccgt
     3661 acagagagca agccaggcgc gcagtagcgc ctcctgcgga aagccgggca ggcgattcgc
     3721 gagagtcgag agagctgacg aaaggggaca caagtgcatg agaataggca ttcctaagga
     3781 agtacaggcc ggtgaaacgc gggtggcgct gattcccagc ctggttcctc ttctcatcaa
     3841 ggaccggcat gaggtcctgg ttgaagcagg tgctggagtc ggggcatact tctccgatac
     3901 acagtatctc caggcaggag cctccctggt cgagggtgtc ccagccttgt acgagcaagt
     3961 tgatgtgatt ttcaaggtgc agccaccgca gatgcacccc gccgctcatc tgcctgaagc
     4021 tgagatgatg catgaggggt ccgcctatat cgggttgctt tccccgttca cgcacctgga
     4081 ggtgatggag acatttgccc ggcgtaagat taccgcattc gccatggact ttgttccacg
     4141 cctcacccgc gcccagagca tggatgccct gagcgccatg gctaccgtgg caggctacaa
     4201 agccgtttta ctggcgacag aacacctggg caaaatgttt cccctgttga tgaccgccgc
     4261 aggcacgatc acgcccgcca cggtgctggt gctgggcgcg ggcgtggcag gattacaggc
     4321 cattgcgacg gcaaaacgac tcggtgccag agtagaggcc tttgatgccc gtccggctgt
     4381 gcgcgaacag attcagagcc tgggcgcaac cttcattgag atggagctgc cgcaggatgc
     4441 ggaaacagcg ggcggctatg ccaaagagca atctgccgag tttctcaagc gcgagcagga
     4501 agtcatctgc gcccggttcc ccagggtaga tgtggtcatc agcacggcgc aggtctttgg
     4561 caaacgcgcc ccgctactca ttacgaccga gatggtcaag tcccttcgcc cagggtcggt
     4621 gatcgttgac ctggccgccg agcagggcgg gaattgcgag ctgacccagg caggccagat
     4681 catcaaatat gaagggatca cagtcgttgg cgtcacaaat gtgcctgcca tgctgcccat
     4741 tgatgccagc cagttatacg cccgcaatgt cgtcaacctg ttccgctatc tctatccagc
     4801 aagcggggtt cagcctgata gcgccgatga actggtgaaa ggggtctgtg tgacacgcag
     4861 cggggcaatc gtgcatgaac tgctccaatc atccataaga gagggggtgt ccacatgaat
     4921 agcctgctct ttgagtcgct ctacatcctc gtcctggcag ggttcctggg gtttgaagtc
     4981 atccgcaaag tgccgccctt gctgcatacc ccgctcatgt ccaccaccaa cgcgatctcc
     5041 ggcatctccc tggtcggatc gctcgtcgcg gctggaagcg gtcagagcac tgtcagcacc
     5101 gtgcttggga cgcttgctgt gactgctgct accattaacg tcgtcggtgg cttcctgatt
     5161 accgatcgga tgctcaagat gtttcggcgg aaagagagta aaccacaatg agcgatgtcc
     5221 tcctggccgc tgcgtacctg gcggcggcca tcctgtttgt cctgggactg agagggttga
     5281 attccccgca gaccgcccgg cgcggcatcc tgctggccga gatcggcatg ctgctcgcgg
     5341 tggtgggaac cctcctgcgc agcgagatca tccagtatca atggattctt gtggggcttg
     5401 ctgtaggctc aactatcggc gccctcatgg cgatctttat gccgatgaca gctatgcctc
     5461 aacgaattgc gatctcccac tcctttggag cgctggcggc caccctggtc ggcattgtcg
     5521 aatattatga gcatggcctg accattgatc acggcacgat ggccgcactc gggtttgagg
     5581 tcttgttcgg ttcgctcacc atcactggca gtctgatggc ctttggcaag ttgcaggacc
     5641 tcgtgaagag cgcgcccatc acctatcgct tccagaatgt ctcgaatatc ctcctgtttt
     5701 gtgcgacagt gggcgtcttt gtctttctga ttgtccggcc tgggacgagc gcgctgttct
     5761 atggcatgat cgcggcggga ctgctcttcg gcatcctgat cgtgctgcca attggaggag
     5821 cggatatgcc ggtggtcctg tcgctgctca actcctatgc gggactggca tctgccgcca
     5881 ccgggttggt catttccaat aatgtgctgc tgatcgcggg cgcgctggac ggaacctccg
     5941 gcttcttcct ctcgatgatg atgagccggg cgatgaaccg ctccttcact aacgtgctgt
     6001 tcggggcggt gggagcggtg aaagcgggag tacaggcgcc aggggagggg gaagaggcga
     6061 gaacggtcat gcgctacact ccagaagatg cagcctatat cctggagagc gcccagtctg
     6121 tgattatcat tcctggctac ggcatggcgg tcgcgcaggc ccagcatgcg gtacaggaat
     6181 tggccgcgct cttgattgag aggggcgcaa ccgttcgcta tgccatccac ccggtggcgg
     6241 gacgcatgcc cgg
//
LOCUS       DASZAK010000925         3281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_272921, whole genome shotgun sequence.
ACCESSION   DASZAK010000925 DASZAK010000000
VERSION     DASZAK010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272921"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VGT44_16240"
     CDS             complement(<1..527)
                     /locus_tag="VGT44_16240"
                     /inference="COORDINATES: protein motif:HMM:NF012490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEV2582407.1"
                     /translation="MSVDMPLESATLTQLQQTPIDNVLHIIDEGLVHLPTYRELYYRW
                     ERQQWRAQEIDFSGDRKQWEGMSQNERNMRLYGLSAFFQGEACVTDTLGPYVIAMPDE
                     EMRIFLTTQLVDEARHTVFFARFFKEVLGVDKEKLEDALAVAREYMNAHLKYILIDAL
                     TEVAGHIQREPGNLAR"
     gene            complement(517..2109)
                     /locus_tag="VGT44_16245"
     CDS             complement(517..2109)
                     /locus_tag="VGT44_16245"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV2582408.1"
                     /translation="MDIADLRFLYDLSLYNCLKYDPYTALIYEDGQDVRAYSNIEIAK
                     EAAQLAAGLRTLGIEKGDRVIVMMLNCPEVIISYQAIARAGGVIIPVLPLLKGPEVRY
                     IAENSAAKAVITSAILLPMLRNALEDVPTMQHLISTGVESEERDESRPYAVHAYKDIV
                     ASGAAYADRYLEALDGVSLTPEDTAVILYTSGTTGHPKGVVLTHRNIVSNAVAGQGGD
                     SSEEDEPGGGEVQLAILPLAHAFGLTVSNISYLSGTKIVMHPRFDTTAVLSAIERHRI
                     SAFAGVPAMFVALLYTPDTDKYDTSSLSYCVSGSAPLPLAVLEGFEQKFGCQILEGYG
                     LSEATTVLTSHGQNMPRKPGSVGKPIPGVEVLVVDANAKPVPAGEVGEIIARGPNIMR
                     GYYNMPQETQAALRNGWLHTGDMGRFDEDGYLYIIERKKDLIIRGGFNVYPRDVEEVL
                     NRHPGVIESAVIGIPSERMGEEVKAFVVTRSEVDAETLKAYCRDSLANFKTPSEIEFV
                     HALPRNAVGKIDKKQLRRLHVS"
     gene            complement(2127..2576)
                     /locus_tag="VGT44_16250"
     CDS             complement(2127..2576)
                     /locus_tag="VGT44_16250"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEV2582409.1"
                     /translation="MSILGATVGQQLSALVKPAITQEQLRRYAEASGDYNPIHLDEQA
                     AQRVGLDGVIAHGMLSMAFLGQFVNQQIADIPGARIEHLKVRFLNMVRLGDTLTCLGE
                     VKSQATHDDLSQSIAIECWAQNQDGHKVTTGEAVVIIPAASRKEGNA"
     gene            complement(2593..3078)
                     /locus_tag="VGT44_16255"
     CDS             complement(2593..3078)
                     /locus_tag="VGT44_16255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582410.1"
                     /translation="MSFQICIKTDKSLQQLATEIRNLFSLPPFRQNSFSGEPYCQFEM
                     IGMLILIHRADEEDRDPEVMNYPYCFDLQMSFSDHELNTDEMEYNLQPYYAQLLSFQL
                     GLDTAYHEKQKADHGWKIRYRYCCKNPKWNESVLFGEPGWEPAVIQMSPGPWRAMHPV
                     F"
BASE COUNT          740 a          896 c          853 g          792 t
ORIGIN      
        1 cgcgccaggt ttcctggttc ccgctggatg tgtccggcaa cctccgtaag cgcatcgatc
       61 agaatatatt tcaggtgagc attcatatac tcacgggcca ctgccagggc gtcttccaat
      121 ttctctttgt cgactccgag cacctctttg aagaagcggg cgaagaaaac cgtgtgacga
      181 gcctcgtcga ccagttgcgt ggtgaggaaa atgcgcatct cctcgtcggg catagctatt
      241 acataagggc cgagggtatc ggtgacacat gcctcgccct ggaagaatgc tgagaggcca
      301 tacaggcgca tattacgctc gttctgtgac atcccctccc attgcttgcg atcaccactg
      361 aaatcgatct cctgcgcgcg ccactgctgg cgctcccaac gatagtagag ttcgcgatag
      421 gtgggcaggt gtaccaatcc ctcgtcgatg atatgtaaca cattatcgat gggtgtctgc
      481 tgcaactgtg tcaatgtggc gctttcaagc ggcatatcaa ctgacatgta gcctccttag
      541 ctgtttcttg tcgatcttcc ccacggcatt gcgcggcaag gcatgcacaa actcgatctc
      601 gctgggcgtt ttgaaatttg ccagcgagtc acgacaatag gctttgagtg tttcggcatc
      661 cacctcggag cgagtcacga cgaaggcttt gacctcttca cccatacgtt ctgatggtat
      721 gccaatcact gccgactcga tcactcctgg atgcctgttc aacacctctt caacgtcacg
      781 tgggtagacg ttaaatccac cacgaatgat caaatccttt ttccgctcga taatatagag
      841 ataaccatct tcgtcaaaac ggcccatatc accggtatgc aaccagccat tgcgcagggc
      901 agcctgtgtc tcctgcggca tgttgtaata gcccctcatg atattggggc cgcgcgcaat
      961 aatctccccc acttcaccgg caggtacagg ctttgcgttt gcgtcaacga ccagcacttc
     1021 aacaccaggg atgggcttac caacggaacc gggcttgcgc ggcatgttct ggccatgcga
     1081 ggtgaggact gtcgtggctt ccgaaaggcc gtagccctca aggatctggc agccaaattt
     1141 ctgctcgaag ccctccagga cagcaagcgg caatggcgcg gagccactca cgcagtagct
     1201 caagctgctc gtgtcgtact tgtccgtatc aggtgtatag agcagcgcca caaacatcgc
     1261 aggcacgccc gcgaaggcgc tgatgcggtg gcgctcaatg gcagagagaa ctgccgtggt
     1321 atcaaaacga ggatgcatca cgatctttgt gccagacagg tagctgatat tggatacggt
     1381 cagcccaaag gcatgagcca ggggtaaaat agccagttgt acttcaccac ctcctggttc
     1441 atcttcttca ctcgaatcgc cgccttgccc cgcgactgca ttagaaacga tattgcgatg
     1501 tgtcagtact acccctttcg gatgaccggt ggttccagat gtatacagga tcacggcagt
     1561 atcttccggc gtgagcgaca caccatccaa agcttccagg taacgatccg cgtatgccgc
     1621 accgctcgct acaatatctt tgtaggcatg aacagcgtag ggccgcgatt cgtcacgctc
     1681 ttcgctctct acgcctgttg aaatgagatg ctgcatggtt ggaacatcct ccagcgcatt
     1741 gcgcagcatt ggcaagagga tagcgctcgt aatcactgct ttagccgcgg agttctcggc
     1801 aatgtagcgc acctctggcc ccttgagcag gggcagcact ggaatgatta cgccgccagc
     1861 gcgagcgatg gcctggtagg agatgatgac ctcagggcag ttgagcatca tgacaatgac
     1921 gcggtcgcct ttctctatac ccaatgtcct gagtcccgct gccagttggg ctgcttcttt
     1981 cgcaatctct atattactgt atgcgcgcac atcttgccca tcttcgtaga taagcgctgt
     2041 atatgggtcg tacttcaggc aattgtacag agaaaggtcg tacagaaaac gcagatctgc
     2101 aatgtccatt ttccgcgcct tgtccgtcat gcgttcccct cttttcttga tgcagccgga
     2161 atgattacaa ctgcttcgcc ggtggtcact ttgtgcccgt cctggttctg tgcccagcac
     2221 tcaattgcta tggattggct aagatcgtca tgagtcgcct gggatttgac ttcaccaaga
     2281 caggtgaggg tatcgcctaa acgaaccata ttcaggaagc gcactttgag atgctctatg
     2341 cgcgcacccg ggatgtcagc tatctgctgg ttaacgaatt gtccgaggaa ggccatgctg
     2401 agcatgccgt gagcaattac accatcaagg ccgacgcgct gcgcggcctg ctcatcaagg
     2461 tgaatgggat tgtaatcgcc tgacgcctcc gcgtaacggc ggagttgctc ctgcgtgatt
     2521 gcgggcttga ccagcgcaga gagttgctgg ccaacggttg cgcccaggat gctcattaat
     2581 aatctccttc tgttagaaaa cgggatgcat tgcacgccac ggacctggcg acatctggat
     2641 aacggcaggt tcccaacccg gctcgccaaa taaaacagac tcgttccatt tcggattctt
     2701 acagcagtag cgatagcgta tcttccagcc atggtcggct ttctgttttt catggtacgc
     2761 ggtatccagg ccaagttgaa aactgagcaa ttgcgcgtaa tagggctgca agttgtattc
     2821 catttcgtct gtattgagct cgtggtcgga aaacgacatc tggagatcga agcagtaagg
     2881 gtagttcatt acctccgggt cgcgatcctc ctcatccgcg cgatggataa ggatgagcat
     2941 ccctatcatc tcaaattggc agtagggttc acccgaaaaa gagttttgcc gaaacggagg
     3001 cagtgagaag aggttgcgta tttcggtagc gagttgttgc aatgatttgt ctgtcttgat
     3061 acagatttga aacgacatac aggactcctt ctggaggtgc gaaggttatt gaacctctct
     3121 ctcgttatag ctgacttcat cttacgccgc ttgaggaata ggtgtcaagc aagggacatt
     3181 tgtctgcgat ggaatacaga ctgatatccg ctcatcattg cttttcgccc ccaccccgcc
     3241 caccatagaa gaaagaacaa ggggacaccc cttgaacccc g
//
LOCUS       DASZAK010000926         1899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_235974, whole genome shotgun sequence.
ACCESSION   DASZAK010000926 DASZAK010000000
VERSION     DASZAK010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235974"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VGT44_16260"
     CDS             complement(<1..219)
                     /locus_tag="VGT44_16260"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2582411.1"
                     /translation="MLLELNIKDFAIIDNLHLSLNRLFNVFTGETGAGKSIIIDAVGA
                     LLGSKIGVDFVRAGCERATVEGIFSVGAL"
     gene            complement(273..1121)
                     /locus_tag="VGT44_16265"
     CDS             complement(273..1121)
                     /locus_tag="VGT44_16265"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEV2582412.1"
                     /translation="MQKTIAIFFQGRKQNTSDVAFRLTRMLQQQQHIVRTIDIREESE
                     ETPDRFLVGCDVAIVLGGDGTILHAARLCAFLNIPIVGVNFGRVGFLTELEPDELETS
                     LPYYLNHDPSVWIDRRAMLHAELEQDGRREAFLALNDVVIARGTWPRVVKIEITIDEN
                     HYNTTHSDGIIISTATGSTAYNMAVGGPLLHPQVQSSVITPIAPHLESDRSLIVQPEA
                     MIKLCISTGSQNGVFSADGQMNREIIDGATILVRNSKHVTQFLRRRPPTYFYQIIHAK
                     LDGDEL"
     gene            complement(1247..>1899)
                     /locus_tag="VGT44_16270"
     CDS             complement(1247..>1899)
                     /locus_tag="VGT44_16270"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2582413.1"
                     /translation="DIAEIRQIVREAEQQGIFAVAALTASSMKMAALLHDIGKPATYA
                     VGEDGGIHFYSHPQVGAPIALQITRRLSASTQDRRLAQQVAAHHMRPGQLGQDGPVTP
                     RAIRRYFVDLGPTGIVVALFSLADHLATFGPQPLTAAWERHLAVARLLLASYVRERER
                     ILPPRLLTAEELMRRLKLNPGPLVGQLLDEISEAQAEGRVHSREEALWLAEERLNQ"
BASE COUNT          390 a          581 c          542 g          386 t
ORIGIN      
        1 cagcgcgcca acagaaaaga tgccttcgac ggtggcgcgc tcgcatcccg cgcgcacaaa
       61 gtccacgccg attttgctgc cgagcagcgc cccgacggca tcaatgatga tcgacttgcc
      121 cgcgcccgtc tcgccggtga aaacgttgaa caggcgattg agcgacaggt gcaggttgtc
      181 aatgatggca aaatctttaa tgttgagttc tagtaacatg gcctttacct ggaaaatcgc
      241 aacggattcc ttatactcta catctcttct tcttatagct cgtcaccatc gagtttcgca
      301 tggatgatct ggtaaaaata ggtcggcggc ctgcgccgca gaaactgcgt cacgtgtttg
      361 ctgttacgca ccaggatggt cgcgccatcg ataatctcgc ggttcatctg cccgtccgcc
      421 gagaagacgc cgttctgcga gccggtagag atgcacagtt tgatcatagc ctccggctgc
      481 acgatcaggg agcggtcgga ttccaggtgc ggcgcgatag gcgtgatcac gctgctctgt
      541 acctggggat gcagcagcgg cccgccaaca gccatgttat aggccgtcga acccgtcgcg
      601 gtcgaaatga tgatgccgtc ggaatgggtc gtgttatagt gattctcgtc gatggtaatc
      661 tcgatcttga cgacacgcgg ccacgtaccg cgtgcgatca ccacgtcgtt caacgccagg
      721 aatgcttcac gccgcccatc ttgctcaagc tcggcatgca gcatggcgcg cctgtcgatc
      781 cagacggatg ggtcatggtt gagatagtag ggcaggctcg tctccagttc gtccggctca
      841 agttcggtca ggaagccaac acgcccgaag ttgacgccaa cgatcggtat attcaaaaag
      901 gcgcacaaac gcgccgcgtg caatatagtg ccatcgccac ccagcacaat cgccacgtcg
      961 catccaacca ggaagcgatc aggagtctct tcactctcct cgcgaatatc gatcgtgcga
     1021 acaatatgct gctgctgctg taacatgcgt gtgaggcgaa aggcaacgtc cgaggtattc
     1081 tgtttgcggc cttgaaaaaa aatagcgatc gttttctgca ctactctctc cagcgggcgc
     1141 aaggggcctt gtcgcttcct acaaggtggg cgttcacctc tatgctcttt actcacgcgc
     1201 ccgcagctac ccacgtaatg gtacgatatg cacggcagct tgtcaactat tgattcaggc
     1261 gttcctccgc cagccagagc gcttcttccc tggaatgtac gcgcccttcg gcctgcgcct
     1321 cggagatttc gtcgagcaat tggccgacca gcgggccggg gttgagtttc aggcggcgca
     1381 tcagttcctc agcagtcagc aggcgcggcg gcaggatgcg ctcgcgttcg cgcacgtagc
     1441 tggccagcag cagccgcgcg acagcgagat ggcgctccca ggcagccgtg agcggctgcg
     1501 ggccgaaggt ggccaggtgg tcggccagtg agaacagcgc cacaacgatg ccggtcggcc
     1561 ccagatcaac aaaatagcgg cgaatggcgc gcggcgtcac aggcccatcc tgcccaagtt
     1621 ggccaggacg catatggtgc gcggcaacct gctgcgccag gcggcgatcc tgcgtactgg
     1681 cgctcaaccg ccgcgtgatc tgcagcgcaa tcggcgcgcc cacctgcgga tggctgtaga
     1741 aatggatgcc gccatcttcc ccaaccgcat acgtagccgg tttgccgata tcatgcaata
     1801 gcgccgccat cttcatagac gacgctgtca acgccgccac cgcaaaaatc ccctgctgtt
     1861 ccgcctcccg cacaatttgc ctgatttcgg cgatgtcgt
//
LOCUS       DASZAK010000927         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_342999, whole genome shotgun sequence.
ACCESSION   DASZAK010000927 DASZAK010000000
VERSION     DASZAK010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342999"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="VGT44_16275"
     CDS             <1..1117
                     /locus_tag="VGT44_16275"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HEV2582414.1"
                     /translation="LVLPPLRTADAFPPSRGHTPTLPTRTVGSHQFQGSAYANGPYTV
                     QGNTILDVSGKPYLFHGIGRDSLEYSCSGDGHLDAQELAYMGSGANRYAITYWGANTV
                     RLPLSEGIWLYGQSSQFCGAAQYQALVKQTVDSLTALHLNVILDLQWIDAGGQSGQAG
                     GPWAAPDADSVTFWQQVASIYQSYSNVLFELYNEPHPATWSCWLSGCTLTDTNYSQDC
                     GCNKTLTYASVGMQALVNAVRGAGATNLAIVAGMDWGFNLSLVASYPIQGINVIYDTH
                     PYPYSEKMPSTWDAAFGKISATYPVISAESGEYDCGISFMSQLLAYFDDHHISWVAWA
                     WVVQGSQCGYPLLIQDYRGTPVPGMGQLIYLHLRSYL"
     gene            1168..2382
                     /locus_tag="VGT44_16280"
     CDS             1168..2382
                     /locus_tag="VGT44_16280"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV2582415.1"
                     /translation="MPGQLTRPVRALGVLTGGGDVPGLNAAIKALVYRSDQLGVTFTG
                     LRLGWEGITFLNRSRSREELVFKPDDPATWRGAYLLPLNRLNTRTIDRMGGTILQSTR
                     TNPARTRVGDLPAHLASYGTGHAPDDRVDLTPEVLANLDYLGLDGLVVIGGDDTLSYG
                     AVLSAKGVPVWGIPKTMDNDVPGTEYCIGFQTAISRAAEFINRIRSTAGSHSETVLFR
                     MFGRDAGFTALETALVTWTDRCLIPEIPSNIDMLAGLVVEDRRNPRNYSTIVLSEGAN
                     LGMPVPEVGAADAYGHRKKANVAEFLADQLSARLPGIRFLPIDLTYFLRSGEPEVYDK
                     HMAIYYANLVMHLVEQGMHGVIAAFRDCAYIATDIPGKNLPARRVNPADYHPTRYRPR
                     FEHITGEYRPQE"
BASE COUNT          507 a          802 c          750 g          512 t
ORIGIN      
        1 actcgtttta ccgccgctgc gcaccgccga cgcctttcct ccatcgaggg gtcacacgcc
       61 cactctgccg acgcgcacgg tgggatcgca tcaatttcag ggaagcgctt atgccaatgg
      121 gccatatacg gtacagggca atactattct ggatgtcagt ggcaaaccgt acctttttca
      181 cgggattggg cgcgatagcc tggaatatag ctgcagcggc gacgggcatc tcgatgctca
      241 ggaactggcc tatatgggaa gcggcgcgaa tcgctatgcc atcacctatt ggggagcgaa
      301 taccgtgcgc ctgccactgt ccgagggcat ctggctgtac ggccaatcct cacaattttg
      361 cggcgcggca caataccagg cgctggtcaa acagacggtt gatagcctga cggcgctgca
      421 cttgaatgtc attctcgatt tgcagtggat cgatgccggc gggcaatcgg ggcaggcagg
      481 cggtccctgg gccgcgcccg acgccgatag cgtgaccttc tggcagcagg tagccagcat
      541 ctaccaatcg tattccaacg tcctctttga gctgtacaac gagccgcacc cggcgacctg
      601 gtcgtgctgg ctctctggct gcaccctgac cgataccaac tactcgcagg actgcggttg
      661 caacaaaacg ctgacctacg ccagtgtggg gatgcaggcg ctcgtcaacg cggtgcgcgg
      721 cgccggggcg accaacctgg cgattgtggc cggtatggac tggggcttta acctgagcct
      781 ggttgccagc tatcccatcc agggcatcaa cgtcatctac gatacacacc cctatcccta
      841 ctccgaaaaa atgccgagca cctgggacgc agccttcggc aaaatcagcg ctacttaccc
      901 tgtcatttcc gcggaaagcg gcgagtacga ttgcggcatc agcttcatga gccagttgct
      961 ggcctacttc gacgaccacc atatcagttg ggtcgcctgg gcctgggtcg tgcagggttc
     1021 gcagtgcggc tatcccctgc tgatccagga ttatcgcggc acacccgttc ccggcatggg
     1081 acaattgatc tatctgcacc tgcgcagcta cctgtgatgt gttatgctga tataaggaat
     1141 cattacagat aaagcaggag taattccatg cctggacaat taaccagacc ggtgcgcgcg
     1201 cttggcgtcc tgacgggcgg gggcgatgtg ccgggtctca atgccgccat caaagcgctg
     1261 gtgtatcgct ccgatcagct cggcgtcacg ttcaccggcc tgcgcctggg gtgggagggg
     1321 attaccttcc tgaatcgctc gcgcagccgt gaagagctgg tcttcaaacc ggacgaccct
     1381 gcaacctggc ggggcgctta tctactgccc ctcaaccgtc tcaatacgcg caccatcgac
     1441 cgtatgggcg gcacgatcct tcaatcgacg cgcaccaatc ctgctcgcac ccgcgtgggc
     1501 gacctgccag cgcatctcgc gagctatggt acgggacacg cgcccgacga ccgcgttgac
     1561 ctgacacccg aggtactggc aaacctggac tatctggggc tggatggact ggtcgtgatt
     1621 ggcggcgacg acacgttgag ctatggcgcg gtgctgtccg cgaagggagt gccggtgtgg
     1681 ggtattccaa agacgatgga taacgatgtt cccggcaccg aatactgcat cggcttccag
     1741 acggccatca gtcgcgcggc agaattcatc aaccgcattc gctcgacggc aggctcgcac
     1801 agcgagacgg tgctgtttcg catgtttggc cgcgatgccg gttttaccgc cctcgaaacc
     1861 gccctcgtca cctggactga ccgctgcctg atcccggaaa ttccctcaaa catcgacatg
     1921 ctggccgggc ttgtcgtgga agatcggcgc aacccgcgca actactcaac gattgtgcta
     1981 tcggagggcg cgaacctggg tatgccggtg ccggaggttg gcgcggcgga tgcctatgga
     2041 catcgcaaga aggccaatgt ggccgagttc ctggcggacc aactgtccgc gcggctgccc
     2101 ggtattcgct tcctgcccat cgacctgacc tacttcctgc gcagcggcga gccggaggtc
     2161 tacgacaagc acatggccat ctactacgcc aacctcgtca tgcacctggt cgagcaggga
     2221 atgcacgggg tgatcgccgc tttccgcgac tgcgcctata tcgcaaccga cattcctggg
     2281 aaaaacctgc ccgcgcggcg cgtcaacccc gccgattatc acccgacgcg ctaccggccc
     2341 cgcttcgagc atatcaccgg ggagtatcga ccacaggagt agggtatagt agcacccagt
     2401 gcgaccgcga gggtctccac aggcagccat gaagacgttt gcgaatgctg acgccgcata
     2461 tagtaatccc cagggcgacc tttgcggtcg ccctcgttct ctcagctcca gaggctacca
     2521 aattttcgtc aaggggtacg tataggtttg cccttcaact gttctatacg c
//
LOCUS       DASZAK010000928         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_272983, whole genome shotgun sequence.
ACCESSION   DASZAK010000928 DASZAK010000000
VERSION     DASZAK010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272983"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..781
                     /locus_tag="VGT44_16285"
     CDS             146..781
                     /locus_tag="VGT44_16285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2582416.1"
                     /translation="MESAREDELQLIIRSQQGDVGAFNQLVLRYQQAVYTLTLRMLGD
                     RDVAADVTQETFLAALRAIQSFRHGSSFRAWLLRIASNQSCDHWRRTHRHPVDSLESL
                     TDEDEPYASGVIAALTTTDRQDNPEEALLDQELQQVIQDGLESLPLEQREAVVLCDIE
                     GLSYEEIALATQTTLGTVRSRIARGRGRLRLYLEQHRELLPGKYRLLTDRE"
     gene            802..1572
                     /locus_tag="VGT44_16290"
     CDS             802..1572
                     /locus_tag="VGT44_16290"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV2582417.1"
                     /translation="MTRSENPEHITIEQLSAFLDQQLTPQEQATVDAHLHTCEQCRLA
                     LADLRATVALVRAMPQPALPRSFTLPANVTPFPVQPASELHRAANVPARRQPAARNGL
                     RRSLRFVSAIAATLGLLIVLSGLWNAISPGIGGGASESAAFTTAGQAPSVSPRASTPG
                     IAVGTNVVTQPGSTASGQQRQTKTPVPQPQQTGTPRQGVLQDTGGGHTTQQPPAPFVL
                     PSYLDLGTIEGRLSLGLALALLGIIGLLVTRRRYHRAI"
BASE COUNT          438 a          640 c          563 g          381 t
ORIGIN      
        1 atcgccacta cgataacgcc aggagaggcg accaccaacg ttatcgtagt ggcgatccct
       61 tgcggtcgcc atgcctctca cgattttgca tcaatcctct cacgacttta catcagccct
      121 gaggtcgaga agaggcggcg ataggatgga atccgcgaga gaagatgagt tacaacttat
      181 aatacgcagt cagcaagggg atgtcggcgc gtttaaccag ctcgtgctgc gctaccagca
      241 agccgtctat accctcacct tgcggatgct cggcgaccgc gatgtggccg ccgatgtgac
      301 gcaggagaca tttctggctg ccctgcgcgc cattcagtca tttcgtcacg gctcctcgtt
      361 ccgcgcctgg ctgctgcgca tcgcctcgaa ccaatcctgc gatcactggc ggcgcaccca
      421 tcgtcacccc gtcgactcgc tggagagcct gaccgacgag gacgagccat acgccagcgg
      481 ggtaatcgct gcgctgacaa ccaccgaccg ccaggacaac ccggaagagg ctctgctgga
      541 tcaggaacta cagcaggtga tccaggacgg cctcgaatcg ctcccgcttg agcagcgcga
      601 ggcggtcgtg ctgtgcgata tcgaagggct ctcctacgaa gaaatcgccc tcgccacgca
      661 gacgacgctt ggcaccgtgc gctcgcgcat tgcgcgcggt cgcgggcgtc tgcgactcta
      721 tttagagcag caccgggaac ttttaccggg caaataccgt ctactcactg atagagagta
      781 atactataat ggagcagttg cgtgacacga tctgagaacc ccgaacacat cacaatagaa
      841 caactctctg ccttcctcga tcagcagctt actccccagg agcaggcgac ggttgatgct
      901 cacctccaca cgtgcgagca gtgccggctg gcgctggcag acttgcgcgc gacggtagcc
      961 cttgtgcgcg ccatgcccca accggccttg ccgcgctcct ttacgctgcc agccaacgtc
     1021 acgccatttc cagttcaacc cgcgagcgaa ctacatcgcg ccgcaaacgt gccggcgcgt
     1081 cggcagcccg ccgcgcgaaa cggcctgcgg cgctcgctgc gtttcgtcag cgccatcgcc
     1141 gctacgctgg gactgctcat cgtactttcc ggcctgtgga acgccatatc gcctggcatt
     1201 ggcggcggcg catcagaatc ggctgcattc acaaccgccg gtcaggcccc atcggtctcc
     1261 cctcgcgcat cgactcctgg tatcgccgta ggcaccaatg tcgtcactca gccggggtca
     1321 accgcgagtg gccaacagag gcaaacgaag acgcctgttc cccagccaca gcagaccgga
     1381 acacccagac agggggtgct tcaggacaca ggcggcggcc acacaacgca acaaccccca
     1441 gcgccatttg tgctgccatc ctacctcgac ctgggaacca tcgagggccg tttgagcctg
     1501 ggtctggcgc tggcgctgct cggcatcatc ggcctgctgg tgacgagacg caggtatcac
     1561 cgcgcgatat aaatgcggac tgtctagatt caagcgaaat gtaggggagc aattcattgc
     1621 gcccacctcg tcgtgcatcg gcctgggatg agggcgcaat gaattgctcc cctacatttc
     1681 gcttgaatct agacgaacta ttaggtagac aacacctcct ttgaaagaac tgcgcaacag
     1741 aaccaaccct tgacaccttc atgcattccg tgctatcatt taatcatctg gttaatacaa
     1801 tcaatgattt gattaactca gtgaactaac cagtcaatac acgggaggaa aaagagcaaa
     1861 agtgggtaga gttatacgcg aacgcgacgc cgaggtcgcg cgcgaggcca ttttggaggc
     1921 cgcggaagag gtctttgcgc gcgagggctt tgcgggtgcg cgcattgata cgatcgcggc
     1981 ggaggcaggc tataacaaga gcctgctctt ccactacttc gg
//
LOCUS       DASZAK010000929         6124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_58369, whole genome shotgun sequence.
ACCESSION   DASZAK010000929 DASZAK010000000
VERSION     DASZAK010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58369"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1602)
                     /gene="cysS"
                     /locus_tag="VGT44_16295"
     CDS             complement(<1..1602)
                     /gene="cysS"
                     /locus_tag="VGT44_16295"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV2582418.1"
                     /translation="MRIYNTLSRRVEEIIPRETNLIKMYSCGPTVYRFIHIGNLRTFT
                     MADWIRRAFEYRGYEVLHVKNITDVGHMRQEMVDRGEDKMLAQARKEGKTSRQIAEFY
                     TGAFHEDENKLNIQPAKVFPRATDHIAEMIAIISGLLAKGVACDINGNVYFDIKRFPG
                     YGKLSGNQLENMLAGVREGVVTDRHNPEDFPLWKLAEPDREMAWESPWGRGFPGWHIE
                     CSAMSMKYLGSSFDLHTGGVDNIFPHHEDEIAQSEAFTGEPFANYWIHAQHLLADGQK
                     MAKSTGNAYTRAEIEARGFDAMSLRYFYTTALYRSRLNFTFRALQASQTALERLRGLA
                     YGLLQEADSAALFSQEPLVANAWQERFLREVENDLNMPRAMAVIWAMLADETCSPTAR
                     LRLLLDADRILGFGLQAYLQSDAPQRKLDPRAALDAVPAPIAERVQQRNALRQEEQYP
                     QADRLRQQIAVDGYALRDTPRGSLVLPRRFDDEFTVLSDSTDAPDHTNDPNLFEFSIN
                     LLAHNSRDDLERCIQSICQQSYGRSI"
     gene            complement(1777..2250)
                     /locus_tag="VGT44_16300"
     CDS             complement(1777..2250)
                     /locus_tag="VGT44_16300"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEV2582419.1"
                     /translation="MTRPEFPIEQQLRKDIQDAQRARDQLKLDTLRMALGAIHNLEVA
                     RTDKKNAEYGQPLTEADCLKVLEQEIKKRSQAIPLYRQGKRDDLVDKEQREMDILQGY
                     LQGAQLSDEDLRVIVARLITEQGREFRKVMPLAAKEMKGRAEGARVQRIVKEMTQ"
     gene            complement(2338..3660)
                     /locus_tag="VGT44_16305"
     CDS             complement(2338..3660)
                     /locus_tag="VGT44_16305"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2582420.1"
                     /translation="MHFAEALVQAGMSGDAGEDEASDGVDQVVNRLAPLISQVLGYQG
                     VGITAFKKGTQQPVATAAYGLAPEQERGWREGREGFTIRELVTKTPIEQRLLSGEAVV
                     LDLSTPPFSAHPTIYGARKILLVPMTLEGRVTGVLTCVSDDPTHDYSEEEVALARALA
                     QLAALTLERTRLFQEHTQAQATVLALRETTRLMDEFIGIAGHELRTPLTTIKVSVALV
                     RRQAIKILKYQDSLPAEVTAEVTTMLDHLDRTERQIRMQNRLISDLLDVARIHTNRLE
                     LHPEPCNLLALVRDSVEDQQYVTPARTITLTNNASEEVPVIADADRVRQVINNYLSNA
                     LKYSQADRPVEVCLNLVGGQARLAVRDEGPGLSEEQQRRVWERFYREPGIEVRSGSGI
                     GLGLGLHICRMIIERLDGLVGIESAPGQGSVFWFTLPIISEPTSCSQE"
     gene            complement(3933..4874)
                     /locus_tag="VGT44_16310"
     CDS             complement(3933..4874)
                     /locus_tag="VGT44_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582421.1"
                     /translation="MDVYRALNADVPQLLFTVLHQAGPTVTDEDFTRTVTALLQDISQ
                     QAQKVGLELQHWLDQQAQREAEETARKLKRLGLLAETADTGVVEQALARDYRRVVSRV
                     GTESLLGLQGSGEMAFAEGMPVDPELAKRVLDGNEYMASLGFKPGQGPLHEAWLLFRG
                     SIHYDVMEHVVRDIMGIYRGSHGAHIENIARTSFGTYNLVYQLGESLTADLASDAHTQ
                     EITFAFSKGPFHYSDFRRDVTTLMEQIGERSDLLHASFWQRKLGLGTGKEFVLRLRIP
                     VGEEALREAIGAIAATGRVGKETVVKRGKMLLGRRVL"
     gene            5100..5963
                     /locus_tag="VGT44_16315"
     CDS             5100..5963
                     /locus_tag="VGT44_16315"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2582422.1"
                     /translation="MKIGLGLPANIPGVTGEQVLAWARKADAGPFSSLALIDRLVYSN
                     YDVLISLAAAAGATERIRLMSSVLLAPLRNPAILAKQGASLDALSGGRLTLGMSVGAR
                     EDDYLAAGVPFHSRGKIFDEQLATMHQIWSGQPLSENVSAIGPAPVQPGGPEILLGGY
                     TEAALKRLRRWGNGYIAGGGGPQRADQFFRMAEETWKDAGRPGRPRLVGAAYVALGQD
                     GLERGGAYIRDYYRMIGPMVERMAAALPTTSEAIKGMIGAFADIGADELILWPTTTDP
                     VQIDQLADLVG"
BASE COUNT         1239 a         1769 c         1759 g         1357 t
ORIGIN      
        1 gatcgagcgg ccataactct gctggcaaat gctctgaatg cagcgttcca ggtcgtcgcg
       61 gctgttatgc gccagcaggt tgatggaaaa ctcgaacagg ttggggtcgt tggtatgatc
      121 tggcgcatcg gtggagtcgg atagcacggt gaattcgtca tcgaagcggc gcggcaagac
      181 aagcgagcct cgcggcgtat cgcgcagcgc atagccatca acggcgatct gctggcgcag
      241 ccggtcggcc tgcggatact gctcctcctg tctcaatgcg ttgcgctgct ggacgcgctc
      301 cgcgatggga gcaggcacgg cgtcgagagc ggcacgcggg tccagcttgc gctggggcgc
      361 gtcgctctgc aggtacgctt gcaagccaaa gccgaggatt cgatcagcat cgagcaagag
      421 gcgcaggcgg gcggtaggcg aacaggtctc gtcggcgagc atggcccaga tcaccgccat
      481 ggcgcgcggc atattcaggt cgttctccac ttcccgcaaa aatcgctcct gccaggcgtt
      541 ggctacgagc ggctcctggc taaagagtgc ggcagagtct gcctcttgca gcagcccgta
      601 ggccagcccg cgcaggcgtt cgagggccgt ctgcgaggct tgcagggcgc ggaaggtgaa
      661 gttgaggcgg ctgcgataga gcgcggtggt atagaagtaa cgcagcgaca tggcatcgaa
      721 gccgcgcgcc tcgatctcgg cgcgggtgta ggcgttgccg gtcgatttgg ccattttctg
      781 cccatcggcc agcaggtgct gcgcgtgtat ccagtagttg gcgaacggct cgcccgtaaa
      841 agcttcgctc tgcgcgatct cgtcttcgtg atgggggaag atgttatcga cgccgccggt
      901 gtgaaggtcg aagcttgagc cgagatactt catcgacatg gcgctgcatt cgatatgcca
      961 gccgggaaat ccacgccccc agggactctc ccaggccatc tcccgatctg gctcggcaag
     1021 cttccagagc ggaaaatctt cgggattatg gcgatccgtc acaacccctt cgcgcacccc
     1081 tgccagcatg ttttccagct ggttgcccga gagcttgcca tagcccggga agcgtttgat
     1141 gtcgaaatac acgttgccat tgatgtcaca ggccacgccc tttgccagca atcccgatat
     1201 gatcgcaatc atctcggcaa tatggtccgt cgcgcgcgga aacaccttcg ccggttgaat
     1261 attcagcttg ttctcgtctt catgaaacgc tccggtataa aattccgcga tctgccgcga
     1321 tgttttgcct tctttgcgcg cttgagccag catcttatcc tcgccgcgat ccaccatctc
     1381 ctgccgcata tgcccgacat cggtaatatt cttgacgtgc agcacttcgt agccgcgata
     1441 ctcaaaggcg cggcgaatcc agtcggccat cgtaaaggtg cgcaggttgc cgatgtggat
     1501 gaagcgatag acggtggggc cgcaggaata catcttgatg aggtttgttt cacgcgggat
     1561 aatttcttca acgcgacggc tgagggtatt gtagattctc atgcgttagc tttgtcctca
     1621 acaggtggca cgacagggaa gccatttcat atacaagcaa gaacacgcaa aggctcgtac
     1681 catattccat ctgaacaaac attcgattca tatggtacga gccgatatca agctttgtca
     1741 agctaatccg tgtaattgct taatgaccgc gagggttcat tgcgtcattt ccttgacgat
     1801 gcgctgcaca cgcgcgccct ccgcgcggcc tttcatctcc ttcgccgcca ggggcatgac
     1861 tttgcgaaac tcccttccct gttcagtgat caacctggct acgatgacac gcagatcctc
     1921 atcgctcaac tgcgcaccct ggagataccc ctgcaaaatg tccatttcgc gctgctcttt
     1981 gtcgacgaga tcgtcgcgtt taccctgccg atagaggggg atggcctggc tgcgcttctt
     2041 gatctcttgc tccaacactt tcaagcaatc ggcctcggtc aggggctgac cgtactcggc
     2101 gtttttctta tcggtgcgcg ccacttccag gttatggatc gcgcccaacg ccatacgcag
     2161 cgtatccagc ttcaactggt cccgcgcgcg ttgggcatcc tggatatctt tacgcagttg
     2221 ctgttcgatg ggaaattctg gtcgtgtcat gtctacacct cttctcgatc attttcatat
     2281 aggactgagt atagcattaa aggtgatgcc gggcaacttt ttttatttgg ggttagctta
     2341 ttcttgtgaa cagctagttg gttcactgat tatcggcagc gtgaaccaga agaccgagcc
     2401 ctgccccggt gcgctctcga taccgaccag tccatcaagg cgctcgatga tcatccggca
     2461 gatatgcagc cccagcccca ggccaatgcc ggagccgctc ctgacctcta tcccaggctc
     2521 gcgatagaac cgctcccaga cgcgccgctg ctgctcctca gacagaccag gaccctcatc
     2581 gcgcaccgct aggcgagcct ggcctccgac aagattgaga caaacctcca caggcctgtc
     2641 cgcctgggag tacttgagcg cgttagagag gtagttgttg atcacctgcc gcacgcggtc
     2701 ggcatccgct atgaccggaa cctcctcgga ggcgttgttg gtgagcgtga tcgtgcgagc
     2761 aggtgtcaca tactgttgat cttcgacgct atcgcgcacc aatgccagca gattgcacgg
     2821 ttccgggtgc agctcgagcc ggtttgtatg gatgcgggca acatccagca gatcactgat
     2881 caagcgattt tgcatgcgaa tctgccgttc ggtgcggtcg agatgatcga gcattgtcgt
     2941 cacctcggct gtcacctcgg cggggaggga atcttgatac ttgaggattt tgatggcctg
     3001 tcgcctgacc agcgcgacgc tgaccttaat ggttgtgagc ggtgtgcgta gttcgtgacc
     3061 cgcaatgcca atgaactcgt ccatcaagcg ggtggtttcg cgcagggcca gcacagttgc
     3121 ctgcgcctgg gtatgttcct ggaaaaggcg cgtccgttcc agggtgagag cggccagttg
     3181 cgccagcgct cgcgccagtg ccacttcttc ctctgaataa tcgtgcgtgg gatcatcaga
     3241 aacacatgtc agcacacccg tcacgcgccc ctccagggtc attggcacca gcagtatctt
     3301 tctagctcca tagatggttg ggtgcgcgga gaacggaggc gtactcaggt ctaacacgac
     3361 tgcttcaccc gacaaaaggc gttgctcaat cggggtcttc gtcaccagtt cacggatggt
     3421 gaagccctcg cgcccctcgc gccacccgcg ctcctgctct ggggccagac cataggcagc
     3481 ggtagccaca ggctgctgcg tccccttttt gaaggcggta ataccaacac cctgataacc
     3541 gagtacctgg ctgataaggg gagcaaggcg atttactacc tggtctaccc catcgctcgc
     3601 ctcgtcttcg ccagcatccc cggacatgcc cgcctgcaca agcgcttcgg cgaaatgcat
     3661 gagcgcctgc agggcttttg aagcagactt cctcagaagg ggatcaggaa gcaagggacg
     3721 ttgggggtcc cctgaaaggg taggtgagcc ttgctcttca tgttccattg gaacgtcctc
     3781 gtaactatgc atgcccctga ccacgttgcc gaaactacta cagaatagcc gaggtgcgtc
     3841 ttttacatat caaattcatc cattccacgc tagtacaggg tacgatagag caagccaaat
     3901 gtaacaggta aagcgcaccc ggcctgatat tcctagagca ctcgtctccc taacagcatc
     3961 ttgccgcgtt tgacaaccgt ttccttgcca actcgtccag tagcggcaat cgctccaatc
     4021 gcctcgcgca gtgcctcttc gcctactgga atgcgcagac gcaatacgaa ttctttgccg
     4081 gtaccgaggc ctagtttgcg ctgccagaac gaggcatgga gcaggtcgct tcgctcgcca
     4141 atttgttcca tcagggtagt cacatcgcgt cggaagtcgc tgtagtggaa aggacctttg
     4201 ctgaaggcga aagtgatttc ctgggtgtgc gcatcgctag cgagatcagc ggtcaggctt
     4261 tcaccgagct ggtagacgag gttgtaggtg ccgaacgagg tgcgcgcgat attttctatg
     4321 tgcgcgccgt gactgccacg gtaaatgccc atgatatcgc gaacgacatg ctccatgaca
     4381 tcgtaatgga tcgagccgcg aaagagcagc caggcctcat ggagcggccc ctgtccgggc
     4441 ttgaacccca gtgatgccat gtactcgttg ccatctagaa cgcgcttcgc cagctcgggg
     4501 tcgactggca tcccttccgc aaaggccatt tcgcctgatc cctgcaatcc caggagcgat
     4561 tctgtgccga cgcggctcac cacgcggcga tagtctcttg ccagggcttg ctcgacaact
     4621 cctgtatcgg cagtttcggc gagcaagcca agacgtttca gcttgcgagc cgtctcttcg
     4681 gcttctcgtt gggcctgttg atcgagccag tgctgcaatt ccaatccaac tttctgcgcc
     4741 tgctggctga tatcctggag cagcgcggtc acagtgcggg taaaatcttc gtccgtcacc
     4801 gtcgggcctg cctggtgcaa aaccgtaaag agcaactgcg gcacatcagc attgagcgcg
     4861 cgatacacgt ccatgttctg ctctcctcac gaactggtaa cattcactcg tagtatagat
     4921 gtaggaaaaa gttgttgcca tccttttctg atagtttatt tacaagtagc tcctacaccc
     4981 tcatattacg atagtatacg ataactcgcg gtctgggtga tacaatgatg gatacagaaa
     5041 gcagctatct tccctttgcg gaagagcaga actgaatcac aaatcaatga ggatagcata
     5101 tgaagattgg tctaggttta ccggcaaaca tccccggcgt cacgggtgag caagtcctgg
     5161 cgtgggccag gaaagccgat gcgggtccgt tctccagcct ggcattgatc gaccggctgg
     5221 tctactcaaa ctacgatgtg ctcatctcgc tggctgcggc ggctggcgcg accgaacgca
     5281 tcaggctgat gagcagtgtg ctgctcgcgc cgctgcgcaa ccccgctatc ctggcgaaac
     5341 agggcgccag tcttgacgcg ctctcaggcg ggcgactgac gcttggcatg agcgtcggcg
     5401 cgcgcgagga cgactacctg gctgccggag ttcccttcca cagtcgcggg aaaatcttcg
     5461 atgagcagtt ggcgaccatg catcagatct ggtcgggtca accgttaagc gaaaacgtca
     5521 gcgccatcgg tcccgctccc gttcagccag gcggccccga aatcctcctc ggcggctata
     5581 ctgaggctgc tttgaaacgt ctgagacgct ggggcaacgg ctatattgcc ggtggcggtg
     5641 gtccgcagcg cgccgatcaa tttttcagga tggccgagga gacctggaag gacgcaggca
     5701 ggccaggcag accccggtta gttggggcgg cttacgtcgc tctgggtcag gatggtttag
     5761 agcgcggggg cgcgtacatc cgcgattact atcgcatgat cgggcctatg gtcgagagga
     5821 tggctgctgc gttgccgaca acgtctgagg ccatcaaagg tatgataggc gctttcgccg
     5881 atatcggagc cgacgaattg atcttgtggc caaccacaac cgacccagtc cagatcgatc
     5941 agctcgctga tctcgttgga tagagcttgc acaaagttta ggagatggta tgcccacgca
     6001 agatatttat cctatcggta cgcttccgcc ggtgggagag gttccacctt atatgtacgc
     6061 gcaagtgatc cggcaggatc ggctgggtga gccgcgcgac gccttcaaga tcgagcgcat
     6121 ggag
//
LOCUS       DASZAK010000930         6908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_199309, whole genome shotgun sequence.
ACCESSION   DASZAK010000930 DASZAK010000000
VERSION     DASZAK010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6908
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199309"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..999
                     /locus_tag="VGT44_16320"
     CDS             130..999
                     /locus_tag="VGT44_16320"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2582423.1"
                     /translation="MHRTTYDSIRLNDRADTPLTERRAFSPLLRVTFIALWILGFVTL
                     MIWSVIIRNHPAPYPFEYQFTLMIQNAQLWGWVDPLIRFFSNLDNTLPAVTGITLCVF
                     ALAFIRWSEQRKEERRYGANARPRGFREHAEWYRQPIFLAVGTAIGSLLNLAITILVA
                     RPRPLGPICGHPHALTAICVTDSIPIHSFPSGHCEFDMMLYGFLLYLSFTRAVQEWRY
                     HWLLLPLQLFAAAAILIIGYSRVLEGDHWLLDVGGGYLSGALWLTLSIFLYRKVRRGA
                     EARWGRKIALSGS"
     gene            complement(1012..4032)
                     /locus_tag="VGT44_16325"
     CDS             complement(1012..4032)
                     /locus_tag="VGT44_16325"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV2582424.1"
                     /translation="MAARDQRKAQAARAGDANREKSGADAGFTSRVSKAMGAIGSEKV
                     RVEQRAGTSASDVKPRSMPKASASKNAATARRVQGQQPGGKKSPRSARRPTPGASSSP
                     SPQTVDSRIIGKAHDKWRSMPPHHQQRVYSILLLALSVLLLGALTFWHTVPLLKQTGT
                     FFLLFFGWGAYPLAFGLILFALFHLVEGMRNARFVRWSVAIGLLAMMLLLLAESALLD
                     KGTGGVIGGLLSGPLLGWPAQVQQVVVIGLLTLAAILTFRIKLSHIGSIFGLFKRLFA
                     DPQRASARDGAGYQSPFLGQRPKFSRYTGSTDDSDEVSQPDARDGGRGVGKSVNGRAR
                     AEAIVEEDDEEDGIDFDADFDVGDDPANDINLHRKEVGSIPRGAGPAVQPLDMDEHAS
                     AVRGARQQALPFGEAGAHGQRAGSDYPQMEPLAPNPRLAKLPKILTRAAKPEVESPWK
                     LSDSALLNNPEEVKVHVLGDDTTTLARVIQETLRSFRVEAEVKAEDISIGPTVIRFGI
                     RPTGKPTMKTDEKGRLVPVRDAAGNIVYEQRTRVSRIMALQNDIALVLEAKSIRMEAP
                     VPGRPYVGVEIPNKNSRLVTLREIFDSKEYAAAKAKSKLALALGKDVAGAVRVGDLAR
                     MPHMLIAGATGAGKSVCLNAFIGSFLMQATPDDVRMLMIDPKMVELNMYNGIPHLLAP
                     VVTEVDKVVGLLKIAIAEMEKRYRLFSQLSVRNLEGYRKLRAERMAHGDNTLKNLPAI
                     VIIIDELADLMMAAPEEVEGLICRLAQLARATGIHLIIATQRPSVDVITGLIKANIPT
                     RISFMVSSAVDSRTIIDMGGAERLLGRGDMLYLPADAGKPERIQGAFVADDEAERLVA
                     YWKQQAAEHAATLAAAAGQKGQGAVSAVPPAVEPGWELSEHISDEYELDDELFEQAEQ
                     VVQEYERASISLLQRRLRIGYSRAARLIDLLEERGVIGRIEPGGRAREVYDKRGGEGR
                     TMADEAADIIAEERAKEEFLKKQAGRNGQQNEDR"
     gene            complement(4109..5770)
                     /locus_tag="VGT44_16330"
     CDS             complement(4109..5770)
                     /locus_tag="VGT44_16330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV2582425.1"
                     /translation="MTTERIRLIPLGGLGEVGKNMMVVEYGDDIIVIDSGSMFPDDEM
                     FGVDLVIPDTSYLQDKKKRIRGIFITHGHEDHIGSLPYELPMLDFPPIYATRLTQGLI
                     QVKLKEHKLLEKATINVVTPGDKVQAGECLVEIVRVNHSVPDAVALAIHTPIGTVIHT
                     GDYKFDHTPTDGQPADFGTLARLGNEGVLVMMGDSTRVETPGYTPSERVINDSFDKIF
                     ANAPGRIIIATFASLLSRVQQVVHTAERYERVVALVGRSMVNNVQMAVEMGYLKIPQG
                     MLIRAEDINKFPAERVVIVCTGSQGEPTSALTRIANQDHRLVRIQPNDTVILSATPVP
                     GNEKMVNRTINNLFRQGAEVLYQSIANVHVSGHAAQEELKLMINLLRPTFVMPVHGEY
                     RQLILHGKLAYGLGIAEDHIVIGEDGDIIEVTETSIKVADHTTCGNVFVDGLGVGDIG
                     QIVLRDRQVLAQDGILMAILTVDKETGQPLAGPDIVSRGFVYMRDAEELLESARERIR
                     ESFDGLNGHASDWSFVKDKIRHTLSEYLYEKTHRRPMILPVVVEV"
     gene            6222..>6908
                     /locus_tag="VGT44_16335"
     CDS             6222..>6908
                     /locus_tag="VGT44_16335"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2582426.1"
                     /translation="MPETTQLDQKLKRIRLKIEEGQEAEALATLDTLAAETPKEERDI
                     VFTRAWYFAHKEQWNTVITFLSPFYNADEIEANWHAASLRERERRAIYLLWMGNAAFN
                     FSYYEDAAYYFNYCLKILDLRRVHLPAVRIKALLGYAMTCIPLGLFPSAILHYEQALQ
                     VTKKEKLEDDLSDIYYGLAEAHRQSGHFDQAYTYGKAALAIYQDRGNRPLVCRMLNLL
                     GRIAYQSGDYS"
BASE COUNT         1457 a         2158 c         1776 g         1517 t
ORIGIN      
        1 tagaaagctg ctgagcgaac tggagagcga gcttgaagcc gagggcaaca cttaatctgt
       61 gcaaccgaat gagctaatgc atccgtgtat atcacaaata ccgatcgcat tactttacaa
      121 ggacgtagta tgcacagaac aacctatgac agcatccgct taaatgaccg cgcggataca
      181 ccactaacag aacgccgcgc gttctctcct ctgcttcgcg tgaccttcat cgcgctctgg
      241 atactcggct ttgtgacgct catgatctgg tctgttatca tccgcaacca tcccgcgccc
      301 tatcctttcg aatatcaatt caccctgatg atccagaacg cgcagttatg gggatgggtt
      361 gacccgctca ttcgattttt cagcaacctc gacaatacct tgcctgccgt caccggcatt
      421 actctttgcg tgtttgccct ggcatttatc cgctggtctg aacagcgtaa ggaagaacgt
      481 agatacgggg caaacgccag gcccagagga ttccgcgaac acgcggaatg gtatcgccag
      541 cccattttcc tggcagtggg gaccgccatc ggcagcctgc tcaacctcgc aataactatc
      601 ctggtagcgc gcccacgtcc tttagggcca atctgtgggc atccacacgc gctgaccgct
      661 atctgcgtca ccgacagcat ccccatccac agttttcctt ccggccactg cgagttcgat
      721 atgatgctct acggcttcct cctctacctc agcttcacga gggcggttca ggaatggcgc
      781 tatcactggc ttctgttgcc attacagttg ttcgcggcag cagctatcct cattatcggc
      841 tattcgcgtg tgcttgaagg cgatcactgg ctgctagacg tgggcggagg ctatctctca
      901 ggcgcactct ggctcaccct ctccattttc ctctatcgca aagtccgcag gggcgccgaa
      961 gctcgttggg gaagaaaaat agcattgtca ggcagctaaa accgcctgct atcaacgatc
     1021 ttcattctgc tggccgttcc tgccagcctg cttcttcaaa aactcctctt tcgctcgctc
     1081 ctcggcgata atatcggcgg cttcgtcggc catcgtgcga ccctcgccac cgcgcttatc
     1141 gtagacctcg cgcgctcggc cacccggctc gatgcgcccg atcacgccgc gttcctccag
     1201 caggtcgatc agccgcgcgg cgcgtgagta gccgatgcgc aagcggcgct gtagcagcga
     1261 gatcgaggcg cgttcgtatt cttgcacaac ctgctcggcc tgctcgaaaa gttcatcatc
     1321 cagttcatat tcgtcggaga tatgctcgct gagttcccag cccggctcga ctgccggggg
     1381 aaccgctgac acagcgccct gccccttctg gcctgcggca gccgccagcg tcgccgcgtg
     1441 ttccgccgcc tgctgcttcc agtacgcgac cagtcgttcc gcctcgtcgt cggccacaaa
     1501 cgcgccctgg atacgttcgg gcttgcccgc gtcagccggc aggtacagca tgtcgccgcg
     1561 ccccagcagg cgttccgcgc cgcccatatc gatgatcgtg cgcgaatcga ccgccgaact
     1621 gaccatgaac gagatgcgcg tcgggatatt cgccttgatc aggccggtaa tcacatccac
     1681 cgagggacgc tgggtggcga taatcaggtg aatgccggtc gcgcgcgcca gctgcgccag
     1741 gcggcagatc aagccttcta cctcttccgg cgcggccatc atcaggtcgg ccagctcgtc
     1801 gataatgata acgatggccg ggagattttt cagcgtatta tcgccgtgcg ccatgcgttc
     1861 agcgcgcagc ttgcgatagc cttccaggtt acgcacactc aactgtgaga agagccggta
     1921 gcgtttctcc atttcggcaa tcgcgatctt gagcagcccc accactttgt ccacctccgt
     1981 caccaccgga gcaagcaggt gaggaatgcc attatacatg ttcaattcga ccattttcgg
     2041 gtcgatcatc aacattcgca catcatccgg cgtggcctgc atcagaaagc tgccaatgaa
     2101 ggcgttcagg cacactgatt tccccgcgcc ggtcgcgccc gcgatcagca tgtgcggcat
     2161 ccgcgccagg tcgccgacgc gcacagcgcc cgccacatct ttgcccagcg ccagcgccag
     2221 cttcgatttg gcctttgccg ccgcatactc tttactatcg aagatttcgc gcagggtcac
     2281 gagacggcta ttcttgttcg gaatctccac accaacgtag ggacgccccg gcacaggcgc
     2341 ctccatgcgg atcgatttgg cctccagcac cagcgcgatg tcattctgca acgccatgat
     2401 gcggctgacg cgcgtgcgct gctcgtacac gatattcccg gcggcatccc tgaccggcac
     2461 caggcgacct ttctcatcgg tcttcatcgt cggtttgccc gtcgggcgaa tgccgaagcg
     2521 aatgacagtc gggccgatgc tgatatcctc cgccttcacc tcggcctcga ctcgaaaact
     2581 gcgcagcgtt tcctggatga ccctcgccag cgtcgtggta tcatcgccaa gtacgtgtac
     2641 cttgacttcc tctggattat tcagcagcgc tgaatcggag agcttccacg gcgactcaac
     2701 ctccggtttt gccgcacgtg tgagaatttt gggaagcttc gccagccggg gattgggcgc
     2761 aagcggctcc atctggggat agtcactgcc agctcgctgc ccatgcgcgc ctgcttcccc
     2821 gaacggcagc gcctgctggc gcgccccgcg caccgctgat gcgtgttcat ccatatcgag
     2881 gggctgaacc gccggtcccg ccccgcgtgg aatgctgccg acctctttgc gatgcaggtt
     2941 gatatcgttc gccggatcat cgccgacgtc aaagtccgcg tcaaaatcga tgccgtcttc
     3001 ttcgtcatct tcctcgacga tcgcctcagc ccgcgctcgc ccattcacgc tcttccccac
     3061 gccccgtcca ccgtctcgcg cgtccggctg actcacctcg tcagaatcgt ccgtagagcc
     3121 ggtatagcga ctaaacttgg gacgctggcc gagaaacggc gattgatagc cagcgccgtc
     3181 tcgcgctgat gcgcgctgtg gatcagcaaa taagcgcttg aacaggccaa aaatcgaccc
     3241 gatatgtgaa agcttgatgc gaaacgtcaa aattgccgcc agcgttagca ggccaatcac
     3301 caccacctgt tgcacctgcg cgggccagcc aagcagtgga ccggagagca gcccaccgat
     3361 taccccacct gtgcctttat ctagcagcgc gctctcagcc aacaggagga gcatcatcgc
     3421 cagcaggccg atagcgaccg accagcgcac aaagcgcgcg tttcgcatgc cttcgaccag
     3481 gtggaacagg gcaaaaagga ttagaccaaa cgccagtgga tacgcccccc agccgaaaaa
     3541 gaggagaaag aacgtgccgg tctgtttcaa caggggaacc gtgtgccaga aggtcagagc
     3601 gcccagcaac agcaccgaca acgccagcaa caggatgctg tagacacgct gctggtggtg
     3661 cggcggcatc gatctccatt tatcatgcgc tttcccgatg atacgcgagt ccactgtttg
     3721 cggggaaggg gaagaactgg cgccaggggt cgggcgacgc gcagatcgtg gcgatttctt
     3781 gccacctggc tgctgtccct gaacgcgtcg cgctgtagca gcatttttgc tcgcgctggc
     3841 tttgggcatc gagcgtggct tgacatcgga cgccgacgta cccgcgcgct gctcaacgcg
     3901 caccttctcc gagccaatag cgcccatcgc cttgctaaca cgagacgtaa agcccgcatc
     3961 cgctccggat ttctctcgat tcgcgtcgcc tgcgcgcgct gcctgcgctt ttcgctggtc
     4021 tctggccgcc attccgggtg cctccttcca acaagcgtaa cgaagataca ctcatgatag
     4081 cgcatttgtt ccagagaagt caacagcgct acacttcgac caccaccggc aggatcatcg
     4141 gtcggcgatg cgtcttctcg tagaggtact cgctgagtgt gtgccggatt ttatccttca
     4201 caaacgacca gtcggaggcg tggccattca gtccatcaaa tgactcgcga atgcgctcgc
     4261 gtgcgctttc gagcaattcc tcggcatcgc gcatataaac gaagccgcgc gaaacaatat
     4321 cggggccggc cagcggttgg cccgtctctt tatcgacggt cagtatcgcc atcagaatac
     4381 catcctgagc aagtacctgg cggtcgcgca gtacaatctg cccgatatct ccgaccccca
     4441 ggccgtccac gaaaacattg ccacaggtgg tatgatcggc gaccttgatc gacgtttccg
     4501 tcacttcgat aatatcgcca tcttcaccaa tcacaatgtg atcttcagca atacccagcc
     4561 cataagccag cttcccatgc aggatcaact ggcgatactc accgtggacc ggcataacga
     4621 aggtgggtcg cagcaggttg atcatcagct tcaattcttc ctgggcagca tggccggaaa
     4681 catgcacatt ggcgatggac tggtataaga cctcagcgcc ctggcggaag aggttgttga
     4741 tggtgcggtt caccatcttc tcattgccgg gaacaggcgt cgccgaaaga atgaccgtat
     4801 cattcggctg gatgcgcacc aggcggtgat cctggttggc gatgcgcgtc agcgccgagg
     4861 tcggctctcc ctggctgccg gtgcacacga tcaccacgcg ctccgccggg aatttattga
     4921 tatcctcggc acgaatcaac ataccctggg gaatcttcag gtagcccatc tccactgcca
     4981 tctgcacatt gttgaccatg ctgcgcccaa ccagcgctac tacccgttcg tagcgttcgg
     5041 cggtatgcac cacctgctgc acacgcgaaa gcagcgaggc aaaggtggcg atgataatgc
     5101 gccccggcgc gttagcgaaa attttgtcga acgaatcgtt gataacgcgt tcggaaggcg
     5161 tatagccggg cgtctcgacg cgcgtgctat cgcccatcat caccagcacg ccctcgttgc
     5221 ccaggcgagc cagcgtccca aaatcggcgg gctgtccatc tgtcggtgta tgatcgaact
     5281 tataatcacc ggtgtgaata acggtgccaa tcggcgtgtg aatcgccagc gcgacagcgt
     5341 cgggaacgct gtggttgacc cgcacaatct ccaccagaca ttcgcccgcc tgcaccttgt
     5401 cgccaggcgt caccacattg atcgtcgcct tttccagcag cttatgctct ttgagcttta
     5461 cttgaatcaa cccctgcgtc aggcgcgtag catagatggg aggaaaatct agcatcggca
     5521 actcatatgg gagactgccg atatggtctt catgtccatg cgtaataaaa atgccgcgaa
     5581 tgcgcttctt cttatcttgc aggtagctcg tatcggggat aacaaggtca acgccaaaca
     5641 tttcatcatc gggaaacatc gaacccgaat caatcactat gatgtcgtca ccatattcga
     5701 cgaccatcat gttcttaccg acttctccca gccctcccag cgggatcagt cgaatacgtt
     5761 cagttgtcaa agtacaacaa acctcctcac tacatccttt tatgtagcgt cactaatctg
     5821 ttcaattgtc gatctatctt cacttctgtc ttcatctaca gaagttaaag ccgtaatgca
     5881 ttcacaaatc atgtcctatt tttccattga acagtgtacc atagatacat gacgaccgca
     5941 agggctgttg ctgccacgct tgcggtcgtc atgcccaacc atgcatcttg tgtaatatat
     6001 cgccgtaggg tatactctat ccaacgggaa tcgcttcttc actctgtggg cgcggtgatg
     6061 aagagatgag atatcccgtt tttatcttcc agtcggctcc cctccttctc aaaaagctca
     6121 tccatcacct ttttttctta tatagtgaca caattctatc tatgcatgag taaatgtgct
     6181 atacttcaaa aaagttgatt atgccgggag ggtgtatttt catgccagag acaacgcagc
     6241 ttgatcaaaa actcaagcgc attcgcttga aaattgaaga agggcaagaa gccgaggcgt
     6301 tggcaacgct cgacaccctg gctgccgaga ctccgaaaga ggagcgcgac atcgttttta
     6361 cccgcgcgtg gtactttgcg cataaggagc aatggaacac cgtcattacg tttctctcgc
     6421 cgttttacaa cgccgatgag atcgaggcga actggcacgc ggccagcctg cgcgagcgcg
     6481 agcgccgcgc catctacctg ctctggatgg ggaatgccgc cttcaatttc tcctactacg
     6541 aggacgccgc ctactacttc aactattgtc taaaaatact cgatttgcgt cgcgtccatc
     6601 tcccggcagt acgcatcaaa gccttacttg gctatgccat gacctgcatc ccgctcggcc
     6661 tctttccttc tgccatcctg cactacgagc aagcgctgca agtgacgaaa aaggaaaagc
     6721 ttgaagacga cctatcagac atctactatg gtttagcaga agcgcatcgc cagtcaggtc
     6781 atttcgatca ggcatacacc tacggcaagg ccgccctggc gatctaccag gacagaggga
     6841 atcgtcccct cgtatgccgc atgttgaact tacttggccg tattgcctat cagtcaggtg
     6901 actacagc
//
LOCUS       DASZAK010000931         1775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_273004, whole genome shotgun sequence.
ACCESSION   DASZAK010000931 DASZAK010000000
VERSION     DASZAK010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273004"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            364..>1775
                     /gene="pckA"
                     /locus_tag="VGT44_16340"
     CDS             364..>1775
                     /gene="pckA"
                     /locus_tag="VGT44_16340"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEV2582427.1"
                     /translation="MTMMMSRKALEDYGLVNLGNIHWNLSPAVLVEHALERKEGTLAS
                     NGAFAATTGSHTGRSPKDKYIVSNAESAAKIWWGEYNHPMSPETFEYVRRRLAAYLQG
                     RDVYVLDAAAGADPHYRMPIQVITELAWHNLFAHQLFLRRNESDLASERPGFTILCVP
                     NFHTDPRLDGTRSDIAIIIDFEERLVLIAGTQYAGEMKKSIFTVLNFILPAEGVLPMH
                     CSANVGPQGDVALFFGLSGTGKTSLSADPSRRLIGDDEHGWGDHGVFNFEGGCYAKCI
                     NLSQKYEPQIWNAVRFGAVYENVVIDPVTREPDYADDSLTENTRAAYPVEFIDDVIKS
                     GTGGQPKAVVFLSADSFGVLPPISRLTTEQAMYYFLSGYTSKLAGTEAGVTTPEATFS
                     SCFGAAFLPLRPGEYANLLRERIEKYQVRCYLINTGWTGGPYGTGERININYTRSMVR
                     AAIDGMLDDVETVTDPIFGL"
BASE COUNT          382 a          520 c          512 g          361 t
ORIGIN      
        1 gaatgtcgac gccatgcagg atggtgtcac caatcccgct cctatcagca cctacgagtg
       61 caacttctgc aatacccacg tcagcgtcta tcccgatgat gacggcggct acctgcgcat
      121 cttcgtcggg gtgtacatga agcacccggt cgcgctttcg tgattccatc gtgattccat
      181 agtctttggt tatagatacg taaagaaatt tgaatgccag acgatgctct tcagcttgac
      241 gtaggatggg ttagatgagt agactaacaa gtggtagggg acagccatcg cgagaaggta
      301 cctgagtatg gtgcttgtga gaacattgtt gttagcgggg cgtcccgaag gagagatagt
      361 cttatgacca tgatgatgag ccggaaagcc ctggaagatt atggattggt gaacctgggg
      421 aatatccact ggaatctgtc gcctgctgtg ctggtggaac acgcactgga acgcaaagag
      481 gggacgctgg cctccaacgg cgcgtttgcc gcgacgacgg gttcgcatac ggggcgttca
      541 cccaaagata aatacatcgt ctcaaacgcg gaatccgccg cgaaaatctg gtggggcgaa
      601 tataatcatc ccatgtcgcc ggagactttc gagtacgtgc gccgcaggct ggcagcctat
      661 ttgcagggac gcgacgtgta cgtgcttgac gcggcggcgg gcgccgaccc gcattatcgc
      721 atgccgatcc aggtcatcac cgagctggcc tggcataacc tcttcgcgca ccagctcttc
      781 ctacgccgta acgaaagcga cctggccagc gagcggcctg gtttcaccat tctgtgcgtg
      841 ccgaacttcc ataccgaccc acgcctggat ggaacgcgct cggatatcgc catcatcatc
      901 gacttcgagg agcggctggt cttgattgcc ggaacgcaat atgcaggtga gatgaaaaaa
      961 tcgatcttta ccgtgctgaa cttcatcttg ccagccgaag gcgtactgcc catgcactgt
     1021 tcggccaatg tcggcccgca gggcgatgtc gcgctgtttt tcggattatc gggcacgggc
     1081 aagacgagcc tgtccgccga tccgagccgc cgcctgatcg gcgacgacga acacggctgg
     1141 ggcgatcacg gcgtcttcaa ctttgagggc gggtgctacg ccaagtgcat caacctttcg
     1201 caaaagtacg agccgcaaat ctggaacgcg gtacgcttcg gcgcggtcta cgaaaacgtg
     1261 gtgatcgatc cggtaacgcg cgaacccgac tacgccgacg actcgctgac cgaaaatacc
     1321 cgcgcggcct acccggtcga gttcatcgat gatgtcatca aatcgggtac aggcggccaa
     1381 ccaaaggcgg tcgtcttcct ctcggcggat tcgttcggcg tgctgccgcc catatcaagg
     1441 ctgacaaccg agcaggccat gtactacttc ctctccggct acaccagcaa gctggccgga
     1501 acagaggcgg gcgtgacgac tccagaggcc acctttagct cctgctttgg cgcggccttc
     1561 ctgcccctgc ggcccggcga gtatgctaac ctgctacgcg agcgcatcga gaagtatcaa
     1621 gtgcgctgct acctcattaa caccggctgg acaggcggcc cctacggcac cggtgagcgc
     1681 atcaatatca actacacccg ctcgatggtg cgcgccgcca tcgacggtat gctcgatgat
     1741 gtcgagacag tcaccgatcc catctttggc ctgca
//
LOCUS       DASZAK010000932         6014 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_522347, whole genome shotgun sequence.
ACCESSION   DASZAK010000932 DASZAK010000000
VERSION     DASZAK010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522347"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /gene="manB"
                     /locus_tag="VGT44_16345"
     CDS             complement(<1..417)
                     /gene="manB"
                     /locus_tag="VGT44_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909030.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEV2582428.1"
                     /translation="MSASQTNIDPTIFSAYDIRGIYGQTLTDDVAYSIGRAAALYLQA
                     PEIAVGRDMRLSSPQLAAALIRGITDQGVNAVDLGMTTTDELYFAVGKFNFPAGVMIT
                     ASHNPGRYNGVKFCRAQAYPISLETGLSDIRDLAIRG"
     gene            complement(421..981)
                     /locus_tag="VGT44_16350"
     CDS             complement(421..981)
                     /locus_tag="VGT44_16350"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2582429.1"
                     /translation="MTETQRRNTYGYIYGERILLRPPTMQDASHVFNWERDEVVWRYD
                     PHRPFSQSMAEFLPIFERNYVRGNGRQFWYIIEDEYQAPIGTITYFNVDYRAGQAEMG
                     LGLGEKSRWGRGYGPEAIRTLTAHLFTVPGMARIYAETAQANHPARRAFVKSGFVEVG
                     PIYDPRSAGEPWMLLEIRKNHYQKGK"
     gene            complement(978..2159)
                     /locus_tag="VGT44_16355"
     CDS             complement(978..2159)
                     /locus_tag="VGT44_16355"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV2582430.1"
                     /translation="MSRLRRAVLVVLDGVGAGANPDARDYGDEGASSLEHCAQAVGGL
                     ELPNLGSIGLGNITPIAGTPPTDHAAGSFGRMAEAGAGKDSTTGHWEMTGVVLRKPLP
                     TYPHGFPPDVVAQFEERIGRKTLGNKAASGTEIIKELGDEHVRTGRPILYTSADSVFQ
                     VAAHQEVIPLEELYHMCQLAREMLSGDHAVGRVIARPFVGTSGHYTRTEHRRDFSLAP
                     LGVSLLDVLKESGKDVIGIGKIEDLFAGCGLTQRDHTETNQDGMAATLRYLARDFAGL
                     LFVNLVEFDMLWGHRRDSQGYAQALREVDSWFAQARQVMRPGDAIFFTGDHGVDPTFR
                     GSDHTREMVPLLAYGEPVRAGVNLGVRASFADLGQTLAQAFGVGPLAAGTSFAGELGL
                     L"
     gene            complement(2156..3358)
                     /locus_tag="VGT44_16360"
     CDS             complement(2156..3358)
                     /locus_tag="VGT44_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV2582431.1"
                     /translation="MASPLNLIIGQSGGATAVINASLVGAVEAALANERIGGVYGMLH
                     GVEGLLKEDVVDLRREPGDVWQRLMYTPSAALGTCRYKLKDEDLARVVEILRRYDIRG
                     LLYIGGNDSADTTHRIAQAAQQAGYDLLAISVPKTIDNDLPVTDHCPGYGSAARFIAL
                     ATMDSTMNTKSIPWHYPVKVIETMGRDAGWLTASAALGKRDEDDAPHILLLPEQPFEQ
                     QRFLRQVEEVYRRVGYVVIVTAEAIRDELGQALGAVGQVGVDAFQHSLLSGAAQHLVE
                     LVIRELGIRARFDKPGDFQRMASNAVSWVDRDEAYLVGRMGALALLEGESDKMVTLLR
                     QPGREYRCTTGLVALSEVANVQRLVPDEYLDASKTMVTRAFYDYALPLIGELLPLYAT
                     LAKQRIAQ"
     gene            3569..4150
                     /locus_tag="VGT44_16365"
     CDS             3569..4150
                     /locus_tag="VGT44_16365"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEV2582432.1"
                     /translation="MAERIFQELSVRSQLDPVINRRMRSHPLKFLSQYDLTFEEVKRL
                     VLPTFTWIVNDKIAAMGFPNSDDAYTVLYKVGIRTIINLTGYNDDAPEIRMFRVHRIP
                     IPNHKAPTIEQMFRATTVITESVKEGLSVLVHCEAGLGRTGTVLAAFFVTVGYDGYDA
                     IETVRSKRAGSIETEEQEQSVLEFAEWFEKSKV"
     gene            4200..5135
                     /locus_tag="VGT44_16370"
     CDS             4200..5135
                     /locus_tag="VGT44_16370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEV2582433.1"
                     /translation="MKHKIILDCDPGHDDAIAILLAARHPEIELLAITTVAGNQTIEK
                     TTRNALKVCSLANIRHVPVAMGADRPLVRTAKPARDIHGESGMDGPHIPEPDIELDRR
                     HGVDLLIELLLNSDGDITLVPTGPLTNIASAIRREPAIVPKIAAISLMGGAIGLGNVT
                     PSAEFNIHTDPEAADIVFRCGRPITMAPLEVTHQALATEAITGRLRAAQRPVTTFAAD
                     LLVFFAGTYRANFGFPAPPVHDPCAVANVIDPTILKAHSMHVEIETTGAWTAGRTVCD
                     VYGKLGLPPNARVGYGLEVDRFWEMVIDTLLSYPS"
     gene            complement(5141..5866)
                     /gene="epsC"
                     /locus_tag="VGT44_16375"
     CDS             complement(5141..5866)
                     /gene="epsC"
                     /locus_tag="VGT44_16375"
                     /inference="COORDINATES: protein motif:HMM:NF041874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEV2582434.1"
                     /translation="MTQRVPDDIDIETMPAQVQPELSGLPEIPPGMYIPVPGTTPRRR
                     TRWPDTIDVIFEKDPACRNIFEALIYQSLWAIIYHRIAHALYRAHIPFLPRFISQFAR
                     FMTGGIEIHPGAKIGKRFFIDHGAGVVIGETAEIGNNVMLYHQVTLGATGWWRPGPGR
                     RQKRHPTIEDDVTIGVGAAILGPITIGRGSKIGAMALVLESVSPNSVVASKPAELLVK
                     GGTPLQHHQELTQGWNGEMDYQI"
BASE COUNT         1229 a         1784 c         1741 g         1260 t
ORIGIN      
        1 gcctcgaatg gccaggtcgc gaatgtcgga aaggcccgtt tccaggctga tcgggtacgc
       61 ctgcgcgcgg cagaacttca cgccgttgta acggccagga ttgtgcgagg cggtaatcat
      121 gacccccgcc gggaagttga atttgccgac ggcgaaatag agttcgtcgg tcgtggtcat
      181 gcccaggtcg acggcattca ccccctggtc ggtgatgccg cgaatcaggg cggcagccag
      241 ttgtggtgat gagaggcgca tgtcgcgccc cacggcgatt tccggcgcct gcaaatacag
      301 ggctgccgct ctgccgatgc tgtacgcgac atcatcggtg agggtctggc catagatacc
      361 gcgtatgtcg tatgcgctaa aaatagtggg gtcgatattg gtttgtgaag cgctcacttg
      421 ctacttccct ttctgatagt gatttttgcg tatttctaaa agcatccagg gttcgcccgc
      481 gctgcgtgga tcgtagatcg ggccgacttc gacgaagccc gacttgacga aggcgcggcg
      541 cgcggggtga ttggcctgcg cggtctccgc gtagatacgc gccatgcctg gcacggtgaa
      601 gaggtgagcc gtcagcgtgc ggatggcttc cggtccgtaa cccctgcccc agcgcgactt
      661 ctcgccgaga cccaggccca tctcggcctg gccagcgcga taatcgacgt tgaagtaggt
      721 gatggtgccg atgggagcct ggtactcatc ctcgatgatg taccagaact gccgcccgtt
      781 gccgcgcacg tagttccgct caaaaatagg tagaaattcg gccatgctct gcgagaacgg
      841 gcggtgcgga tcgtaacgcc agacgacttc gtcccgttcc caattgaaca cgtgcgaggc
      901 atcctgcatc gtgggtggcc gcagcaggat gcgctcgcca tagatatagc catacgtatt
      961 gcgtcgttgt gtctctgtca tagtaacccc aattcaccgg cgaagctcgt gccagccgcg
     1021 agcggcccca cgccgaacgc ctgcgccagt gtctgtccaa gatcagcaaa gcttgcgcgc
     1081 acacccaggt tgactccggc gcgcaccggc tcgccataag ccagcagcgg caccatctcg
     1141 cgcgtgtggt cgctgccgcg aaaggtcgga tccacgccat gatcgccggt gaaaaagatg
     1201 gcgtcgcccg gacgcatcac ctgccgcgcc tgggcgaacc aactatcgac ctcgcgaagc
     1261 gcctgggcat agccctggct gtcgcggcga tgaccccaca gcatatcgaa ctctacaaga
     1321 ttgacgaaca acagccccgc gaagtcgcgt gccagatagc gcagcgtggc cgccatgcca
     1381 tcctggttcg tctcggtatg gtcgcgctgc gtcaggccgc accccgcgaa gaggtcttcg
     1441 atcttgccga tgccgataac atctttgccg ctctccttta atacgtcgag cagcgaaacg
     1501 ccgagcggcg cgagcgagaa atcgcggcga tgctcggtgc gcgtgtaatg gcccgatgtg
     1561 ccgacgaagg gacgcgcgat gacgcggcca acggcgtgat cgccgctgag catctcccgc
     1621 gcaagctggc acatatgata gagttcctcg agcgggatca cctcctggtg cgccgcgacc
     1681 tggaagacgc tgtccgccga ggtatacagg atcgggcgac cggtgcgcac atgttcgtcg
     1741 cccaactctt tgatgatttc ggtgccggag gcggctttat tgcccagcgt tttgcgcccg
     1801 atgcgctctt caaactgtgc cactacgtcg ggcgggaaac catgtgggta ggtcggcagg
     1861 ggcttgcgca gcacaacgcc ggtcatttcc caatggccgg tggtgctatc cttgcccgcg
     1921 ccagcctcgg ccatgcggcc aaacgaaccg gcggcgtgat cggttggcgg cgtccccgcg
     1981 atgggtgtga tattgcccag gccaatgctg cccaggtttg gcagttccag cccgccgacg
     2041 gcctgcgcgc aatgttcaag cgaacttgcc ccctcgtcgc cgtagtcgcg cgcgtccggg
     2101 ttcgcgcccg cgcccacgcc atccagcacg accaggactg cgcgtcgtaa cctgctcatt
     2161 gtgctatcct ttgctttgcc agcgtcgcat agagcggcag cagctcgcca atcaacggca
     2221 gagcatagtc gtaaaatgcc cgcgtcacca tcgtcttgct cgcatccagg tactcgtccg
     2281 gcaccaggcg ctgcacattt gccacctccg acagggccac cagccccgtc gtgcagcgat
     2341 actcccgccc aggctggcgt agtaatgtta ccattttatc agattcaccc tccaacagcg
     2401 caagcgcgcc catcctgccg accaggtacg cctcgtccct gtcaacccac gaaaccgcgt
     2461 tagaggccat gcgctggaaa tcacccggtt tatcgaagcg cgcccgtatc cctagctcgc
     2521 gtatgaccag ttccaccagg tgctgcgccg cgccactcag cagagaatgc tggaaggcat
     2581 cgacccccac ctgcccgacc gcgccaagcg cctgccccag ttcgtcgcgg atcgcctcag
     2641 ccgtgacgat caccacgtag ccaacgcggc gatagacctc ctcgacttgt cgcaaaaaac
     2701 gctgctgctc gaatggttgt tcgggcagca gcaagatatg cggcgcatca tcttcgtcgc
     2761 gcttgcccag agcggcagag gcggtcagcc agccagcatc gcgccccatc gtctcgatga
     2821 ctttgacggg ataatgccag ggaatcgatt ttgtgttcat ggtggagtcc atggtggcca
     2881 gggcgatgaa ccgcgccgcg ctgccatagc ccggacagtg gtcggtcact ggcaagtcgt
     2941 tatcgatggt cttggggacg ctgatggcca gcaggtcata cccggcctgc tgagcggcct
     3001 gtgcgatgcg atgcgtcgta tcggcggagt cgttgccgcc gatatacagc agcccgcgaa
     3061 tatcgtacct gcgcaggatt tcaacaacgc gcgccaggtc ttcatctttg agcttgtagc
     3121 ggcaggtgcc aagcgccgcc gacggcgtat acatcaagcg ctgccagaca tcccccggct
     3181 cccgtcgcag atcaaccacg tcttccttca acagtccttc gacgccgtgc aacatgccat
     3241 acacgccgcc gatccgctcg ttcgccagcg cagcctcgac cgcgccgacc aggctggcat
     3301 tgatgacagc ggtcgccccg cccgattgtc cgatgatgag gttaagaggt gatgccatta
     3361 tgcttctttt ctcacttgtt ctatcttcca cagtcattat atcaagactg agatgaggcg
     3421 tcgaggggac ctaataagct aatcaacaaa accagcaggg cgatgctggc catcgccctg
     3481 gtatccacca taggcgagat tgtctggttg agagattagt gctacaatca aggggcttta
     3541 ttagaatgta gaaaaggcaa acagtccgat ggctgaaaga atttttcaag aactgagtgt
     3601 gcgctcgcag cttgatcctg tgattaatcg taggatgcgt tcacatcctc tcaagtttct
     3661 ttctcagtat gatcttactt ttgaagaggt aaaacggtta gtgcttccta catttacctg
     3721 gatagtaaat gataagatag cagccatggg atttcccaat agtgacgacg cttataccgt
     3781 tttgtataag gtcgggatac gaacaatcat aaatctgacc ggctacaatg atgatgcgcc
     3841 agagatcagg atgtttagag tacaccgaat tccaattcct aaccataagg ctcctacaat
     3901 agaacagatg tttagggcta ctacagttat tactgagtca gttaaagagg ggctgtccgt
     3961 tctggtacat tgtgaagctg gtttaggtcg aacaggaacc gtgcttgctg ctttctttgt
     4021 aacggtagga tatgacggat atgacgcaat agagactgtc cgttctaaaa gggctggttc
     4081 tatagagaca gaagaacagg aacagagcgt attagaattt gcagagtggt ttgagaaaag
     4141 caaagtttag ccaatggttg ggcttccctg gctcacttca taggggaata attatttaga
     4201 tgaaacacaa aatcatcctc gactgcgatc cagggcatga cgacgccatt gccatcttgc
     4261 tggcggcgcg tcatcctgag atcgaactgc tggcgatcac gacggtggcc ggaaaccaga
     4321 cgatagagaa gaccacgcgc aacgcgctca aggtctgctc gctggcgaat atccggcacg
     4381 tgccggtggc gatgggcgcg gaccgcccac ttgtgcggac cgccaaaccc gcgcgggata
     4441 ttcacggcga atcgggtatg gacgggccgc atattcccga accggatatc gaactggatc
     4501 ggcgacacgg cgttgacctg ctaatcgaat tgctgctgaa ctcggacggg gatatcacgc
     4561 tggttcccac cggcccactg accaatatcg cgtcagccat acgccgcgaa ccggccattg
     4621 tccccaagat tgccgcaatt tcgctgatgg gcggggccat cggcctgggg aacgtcacgc
     4681 cctcggcgga gttcaacatc cacaccgacc cggaggcggc ggatatcgtc tttcgctgcg
     4741 gcaggccaat cacgatggct ccgttggagg tgacgcacca ggcactggca actgaggcca
     4801 tcaccgggcg attacgcgcc gctcagcgtc ccgtcaccac cttcgccgcc gacctgctcg
     4861 tcttcttcgc cggcacctac cgtgccaact tcggctttcc cgcgccgcct gtgcatgatc
     4921 cctgcgccgt cgccaacgtc atcgacccca ccatcttgaa ggcgcactct atgcatgtcg
     4981 agatcgagac aaccggagca tggacggcgg gcagaacggt ctgcgatgtg tatggcaagc
     5041 tgggattgcc acccaacgcg cgcgtgggct acggcctgga ggtagaccgc ttctgggaga
     5101 tggtgataga cacattacta tcctacccgt cgtagggacc tcagatctgg taatccatct
     5161 cgccgttcca gccttgcgtt agctcctggt gatgctggag tggtgtgccg cctttgacca
     5221 gcagctcggc tggctttgag gcgacaaccg aattgggcga gacggattcc agcaccagcg
     5281 ccatcgcgcc tattttgctg cccctgccaa tcgtgatcgg tcccaggatg gccgctccga
     5341 cgccgatggt cacatcatct tcgatggtcg ggtggcgctt ctggcggcga ccgggtccgg
     5401 gccgccacca gccggtcgcg cccaaagtga cctggtgata gagcatgaca ttgttgccga
     5461 tctccgccgt ttcgccgatg accacgcccg cgccatgatc gatgaagaag cgtttgccga
     5521 ttttcgcgcc gggatggatt tcgatgccgc ccgtcatgaa gcgcgcaaac tgcgagatga
     5581 aacgcggcag aaacgggata tgcgccctgt agagggcatg ggcaatgcga tggtagatga
     5641 tagcccacag gctttgatag ataagcgctt caaaaatatt acgacaggcg gggtctttct
     5701 caaaaataac gtcgatggtg tcgggccagc gtgtcctgcg gcgcggtgtt gtacccggca
     5761 cggggatgta catgccaggg gggatttcag gcaaccccga taattccggt tgtacctggg
     5821 ctggcattgt ttcgatatct atatcatcag gtacgcgctg tgtcattggt gatgttaccc
     5881 ttttttatcc aatcgcccga tcttcttact ttggagtgta tggcagggcg cgagcaatgt
     5941 caatagtacc gatttgttgt ttacataagc gtaacctgct atagttgcgc tatcaaacac
     6001 ttacatcttt tcct
//
LOCUS       DASZAK010000933         5324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_343056, whole genome shotgun sequence.
ACCESSION   DASZAK010000933 DASZAK010000000
VERSION     DASZAK010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343056"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /gene="tyrS"
                     /locus_tag="VGT44_16380"
     CDS             complement(<1..1129)
                     /gene="tyrS"
                     /locus_tag="VGT44_16380"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV2582435.1"
                     /translation="MRTTGETLESFSRTTEEFISLEEFKRLLDAGRQLRIKYGVDVTA
                     PTLHTGHAVNLWMMRELQDMGHKIQFLIGDYTTRIGDPSGRSKQRPVIPQEEIEANAQ
                     KFIEQVKMVIRFDDPNLYEIRRNSEWYERMSASELIKLLSMVTHSRLIARDMFQARIK
                     EGTEIYMHELIYPVLQGYDSFVLQSDLTIIGSDQLYNEMMARFYQERLGQPPQVIITT
                     RITPGTDGKAKQSKSLGNYIGLAHSPRDKFGRVMTLPDDLIVTYLEVYTDIPLQEVQQ
                     IAGHVADDPMRWKLFLAHAIVRRYHGSEVADQEQRWFIETFSARNTPVNIPEIVVAPG
                     EYTAFDLLKRYFGAQKSNRELRRLFEQGAITLNGSKIERSDQ"
     gene            complement(1344..2495)
                     /locus_tag="VGT44_16385"
     CDS             complement(1344..2495)
                     /locus_tag="VGT44_16385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HEV2582436.1"
                     /translation="MADRSTLHDDEIAFIRSQRVARLATSDGDGHPHVVPVCFAFDGS
                     RFYTPLDEKPKRVEQQRLRRVRNIEARHEAMLLFDEYHEDWSRLGYMQVYGHAGLLMP
                     GEPGHKEALLLLRQRYVQYRSMALEEHPLIVITPRRVVAWGATRKGSTMDFSHFDTRN
                     YPTISAQEGYGAWASTYEGSVLDLMDLRLLERIKSVTWDHIDEAADLACGTGRTGVWL
                     KQKGVASLDGVDFTPEMLEGARAKGVYRQLLMGDIRETPLHAGAYDLVTVDLADEHLS
                     DVQPLYQEAARIARPNGYLVLVGYHPFFLMSGIPTHFNSASGEPVAIESHIHLISDHV
                     QAALAAGWSLLEMHEGLIDDEWLERKPKWNQYRNRPISFAMVWQKQRND"
     gene            2659..4734
                     /locus_tag="VGT44_16390"
     CDS             2659..4734
                     /locus_tag="VGT44_16390"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2582437.1"
                     /translation="MISTQFCPICGAANETTNSQCYACGHHLLPESDQEDLLQHDLLF
                     GRYQLLTILGSGGFSSVYQARDKQTDMTVAIKQINLRGLNAEEMIDATTTFNREASVL
                     STLNHHQVPRLYNQYQDRDHWYLVLEYFDGPTLETYLERRLAQGNPIRFDEAISMSLQ
                     LCAVLDHLHTRQPPIVYRDLKPGNIIRDTSGKLSLVDFGIARNFRPGQSRDTQLLGSP
                     GYAAPEQYGRAQTSPQSDIYSLGVLLYALLSQSDPGSCAPDLSALDLSQHTGGAAMYD
                     LLTRLLAVDPANRPASVREVITALEAIGPRGLAPQNARRIWIPPTPQTYSAASGMQQQ
                     LFIPPQPVQVALPKKKSRVSRRSVIIGLGVLVTSAGVGLSLKSLAAFVTPTPVSKPTP
                     ILIYDKHLTPVSGIAWSTALQLIASCSQNTVRVWDINGDDAFVYSSSDGNDFNGVAWS
                     PDGLHLALASNKMGVVVLNTSTGFVSTNYLQHAGQVNGVAWSPDGSRIASADNDKTVQ
                     VWDAFSGNLLATYQATAGDFSTAEQVVVRAVAWSLDGACLASCTSDGIVRTWDALTGA
                     MFISFDYDTGQSANAIAWSPDNLHIATASDNGTVYILLNSLPGVTPFVFTGHTTRVTA
                     VAWSPDGRRIASADARGIILVWDAYTGKNAVTYTDSPHGVSALAWSPDGQYLASGSQD
                     QTVKIWQVS"
     gene            4952..>5324
                     /locus_tag="VGT44_16395"
     CDS             4952..>5324
                     /locus_tag="VGT44_16395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEV2582438.1"
                     /translation="MSKFTEAELNYLRTQRLGRLATVNERGEPQVAAVGFRYNPEFDT
                     IDIGGRDMAHTQKFRNVARNGLASLLVDDVLPPWKTRSMEIRGHAQALPEGGLSISSS
                     MSPALIRITPRRIIFWDASADP"
BASE COUNT         1127 a         1561 c         1379 g         1257 t
ORIGIN      
        1 cctggtccga ccgctctatt ttgctgccat tgagcgtgat cgcgccttgc tcgaacaggc
       61 ggcgcagctc tctgttgctc ttttgcgcgc caaagtagcg tttcagcagg tcaaaagccg
      121 tgtattctcc tggcgcgacc acgatctcag gaatgttcac gggcgtgttg cgcgccgaga
      181 aggtttcgat aaaccagcgc tgctcctggt cggcgacctc gctgccgtga tagcgtcgca
      241 cgatggcgtg tgccagaaag agcttccagc gcatcgggtc gtcagcaaca tgcccggcga
      301 tctgctgtac ctcttgtaga ggaatatcgg tgtagacctc caggtaggtc acgatcagat
      361 catccggcag ggtcatgacc cgcccgaact tgtcacgcgg actatgagcg aggccgatgt
      421 agttgcccag gcttttgctc tgcttggcct tgccgtcggt gcccggcgta atgcgcgtgg
      481 tgatgatgac ctgcggcggc tgccccagac gctcctggta gaagcgagcc atcatctcgt
      541 tgtaaagctg gtcggagccg atgatcgtca ggtcagattg cagcacgaac gagtcgtatc
      601 cctgcaagac agggtagatc agttcgtgca tgtagatctc cgtgccttct ttgatgcgcg
      661 cctggaacat atcccgcgca atcagcctgg aatgtgtcac catcgagagc agcttgataa
      721 gctccgaggc cgacatgcgc tcgtaccatt ccgaattgcg gcggatttcg tacaggttgg
      781 gatcatcgaa gcgtatcacc atcttcacct gctcgatgaa tttctgggca ttggcctcaa
      841 tctcctcctg tggtatgacc ggtcgttgct tgcttctccc cgatggatcg ccgatgcgcg
      901 tggtgtaatc gccaatcaga aattggattt tgtgtcccat gtcctgcaat tcgcgcatca
      961 tccacagatt caccgcgtgg ccggtatgca gcgtcggcgc ggtcacatcc acaccatact
     1021 tgatgcgcag ttgccgcccg gcatccagca atcgcttgaa ttcctccaac gaaatgaact
     1081 cttccgtcgt tcttgagaag ctctcaagcg tctccccggt cgttctcatc atcctgctcc
     1141 ttctttacca ttccagccaa taaaaaagca gcagaggttg tcactctgct gccctaccta
     1201 tcggtggtta acggaccatt cagcgcctgc gcacgtcaat aatcgctcca cccatatgta
     1261 gggcagaccg gtaataatag ctctgactgt gtgcaaatgc tgtgtgtttc ataacaagag
     1321 tatatgggat ttaggaagtg gtgtcaatca ttccgctgct tctgccagac cattgcgaag
     1381 ctgatgggcc gatttcgata ctggttccac ttcggctttc tctcaagcca ctcatcatct
     1441 atcagcccct cgtgcatttc aagcaaagac cagcctgctg caagggctgc ttgcacatgg
     1501 tcgctaatca ggtggatgtg gctttcaatg gcgactggtt cccccgatgc gctgttgaaa
     1561 tgggttggga taccgctcat caggaaaaag gggtgatagc caaccaggac gaggtatcca
     1621 tttggacgcg cgatcctggc ggcctcctga tagagcggtt gcacatcgga tagatgctcg
     1681 tctgccaggt caaccgtcac aaggtcatag gctccggcgt gtaatggcgt ctcacgtata
     1741 tcgcccatca acaactgccg gtagaccccc ttggctcgcg cgccttccag catttcaggt
     1801 gtaaaatcaa cgccatcgag tgatgcgaca cctttctgct tgagccatac tcctgtacgc
     1861 cctgtgccgc acgccaggtc tgcagcctcg tcgatatggt cccacgtgac tgatttgatt
     1921 ctttccagca aacgcaggtc catcagatcc agcacgctcc cttcataagt agatgcccat
     1981 gctccgtagc cttcctgcgc ggagatggtg ggatagttgc gtgtatcaaa gtgtgaaaaa
     2041 tccattgtac ttccttttct tgtcgctccc catgctacaa cacgccgggg cgtaatcaca
     2101 atcagcggat gctcctccag cgccatcgac cggtactgga catagcgttg gcggagcagg
     2161 agcagcgcct ccttgtgtcc tggttcgcca ggcatgagca acccggcgtg gccgtacacc
     2221 tgcatatacc ccaggcgaga ccaatcttca tggtactcgt caaacaagag catggcctcg
     2281 tggcgcgctt cgatgttgcg cactcgccgc aagcgctgct gctcgacccg tttgggtttc
     2341 tcatcgagtg gcgtgtagaa gcgcgaccca tcgaaggcaa aacaaacagg taccacgtgc
     2401 ggatgcccgt ccccgtccga tgttgccagc cgcgccactc gttgcgagcg aatgaacgcg
     2461 atttcgtcgt catgtaaggt cgaacgatct gccattgcaa tgctcaatcc gtcatagtgt
     2521 atcaggcttt tagtgtatcc acttctggat atacctggca agcacgcata cgatcctgat
     2581 atgagagaat acgctaatcg attgcatact atatagtagc gtattccttt ctacgagggg
     2641 gatgaagaag aggcagtcat gatctctacc caattctgtc caatttgtgg ggcggcgaac
     2701 gaaacgacaa actcacagtg ctatgcctgc gggcatcatt tgctgccaga atcggaccag
     2761 gaagacctat tgcagcatga cctattgttt ggccgctatc aacttctcac tatcctgggt
     2821 agtggcggct ttagttccgt ctaccaggct cgagataaac agaccgacat gaccgttgcg
     2881 atcaaacaaa tcaatctacg cggcttgaac gcggaagaaa tgatcgatgc tacgactacc
     2941 ttcaatcgtg aagcgagcgt gctttcaacg ctcaaccatc accaggtgcc acgtctctac
     3001 aaccagtatc aggatcgcga ccactggtac ctggtgctgg aatatttcga tggtcccacc
     3061 ctggaaacct acctggaacg gcgtctcgcc caggggaacc cgattcgttt cgacgaagcg
     3121 atttcaatga gcctgcaact ctgcgccgtc ctcgatcacc tgcacactcg ccagccgccg
     3181 attgtctatc gtgatctcaa gcccggcaat atcattcgcg acacctcggg caaactttcc
     3241 ctggtcgact ttggcatcgc tcgcaacttt cgtccaggcc aatctcgcga tacccagctg
     3301 ctcggttcgc ccggctatgc cgctcctgaa cagtacggac gcgcgcagac ctcgccccag
     3361 agcgatatct acagcctggg tgtcctgctg tatgccttac tttctcagag cgatcctggc
     3421 agttgcgccc cggacctgtc cgcgcttgac ctgtcgcagc acacgggcgg agcggcaatg
     3481 tatgaccttc tcacgcgcct gctggccgtc gatcctgcga atcggcccgc gagcgtgcgt
     3541 gaagttatca ccgctctcga agccatcggc ccccgcggac tggcacccca aaacgcccgg
     3601 cgcatctgga ttccaccaac gccccagacc tattctgctg cttctggaat gcagcagcag
     3661 cttttcatac ctcctcaacc tgtgcaagtt gcgctcccga aaaagaaaag ccgggtgagc
     3721 aggcgcagtg tcatcattgg cctgggtgtg cttgtcacct ccgccggggt tggcctgagt
     3781 ttgaagtcac tcgcggcgtt cgttactcca actcctgtct ctaagccgac tccgatattg
     3841 atctatgaca agcatctgac acctgttagc ggcattgcct ggtcaaccgc tttgcagttg
     3901 attgcctctt gctcccagaa tacggtacga gtctgggata tcaacgggga tgatgctttc
     3961 gtttacagca gcagtgacgg caatgacttc aatggtgtag cctggtcgcc tgatggcctg
     4021 catctcgcgc tggcctccaa caagatggga gtggtggtcc tgaataccag tactggattt
     4081 gtttccacaa attatcttca acacgcaggg caggtcaatg gtgtagcctg gtcgcccgat
     4141 gggtcgcgca tcgcctccgc cgacaacgat aaaactgtgc aggtctggga tgccttcagc
     4201 gggaaccttc tcgcgacata ccaggccacc gcaggcgact tctccaccgc agagcaggtt
     4261 gtcgtaagag ccgtggcctg gtccctcgat ggcgcgtgcc tcgcttcgtg tacgtcagac
     4321 ggcatcgtac gcacctggga cgctctcacc ggagcaatgt tcatcagttt tgattacgat
     4381 actggccaat ccgccaatgc tatcgcctgg tctcctgaca atctccacat cgcaacagca
     4441 tctgataatg gcactgtata tatcctgttg aacagcttgc caggcgtcac gccttttgtc
     4501 ttcacaggac atacgaccag agtcactgcc gtagcctggt cgcccgatgg caggcgcatt
     4561 gcctctgccg atgccagggg aatcatcctg gtctgggatg cctataccgg taaaaacgcc
     4621 gtcacctata ccgattcccc acacggggtc tccgcgctcg cctggtcacc cgatggccag
     4681 tatcttgcct ccggttctca agaccagacg gtgaagatct ggcaggtaag ctaaacaaac
     4741 acctcaattt ccatcttgca tgatagaagg atcaatcgct tcaactgtgg ctgcgccatt
     4801 cctgttcttt tgaacagggc tactctggct aaagggacga tcttttcccg actcacagat
     4861 gagatactct cccaggagaa gcaaaaacgt ttgctcctcc tggtataatg tgcgtgaaaa
     4921 tgtatcttac tgggaagtca tcaggtgcag tttgagtaaa tttaccgaag cagagctgaa
     4981 ctacttaagg acacaacggc ttggacgact ggcgaccgtc aacgagcgag gtgaaccaca
     5041 ggtggcggcg gtcggcttcc gctacaatcc tgagtttgat accatcgata tcggcggacg
     5101 tgatatggcg catacgcaga agtttcgcaa tgttgcccgt aatggcctgg catctttact
     5161 ggtcgatgat gtactgcctc cctggaaaac acgcagtatg gaaatacgcg gacatgccca
     5221 ggctcttccc gaaggcgggc tgtctatttc atccagtatg agtcccgcat tgatacgcat
     5281 cacccccagg cgcattatct tctgggatgc gtccgcagat cctc
//
LOCUS       DASZAK010000934         3009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_592816, whole genome shotgun sequence.
ACCESSION   DASZAK010000934 DASZAK010000000
VERSION     DASZAK010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592816"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /gene="der"
                     /locus_tag="VGT44_16400"
     CDS             <1..1453
                     /gene="der"
                     /locus_tag="VGT44_16400"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV2582439.1"
                     /translation="MSEAEQGKGRGAQMKPLVAIVGRPNVGKSTFFNRMIGERVAIVE
                     DMPGTTRDRIYGDTDWNGREFTLIDTGGLEFGSDIPIGQVGLNGQPGDLMKHVQAQAE
                     LAIEEADVIVFMVDARGGITAADEEVAEVLRRTNKPVILAANKADNARLRLDAVEFYG
                     LGIGEPIDISSLQGVGTGDLLDAIVEALPPEEEESDGEDEETPRIAIVGRPNVGKSSL
                     LNAILGYPRAIVSDIPGTTRDAIDTELEFEGRKLRLIDTAGIRRRGRVGPGVEKFSVL
                     RSSRAIERADVAILVIDAGEGLAAQDTHIAGEIQDLAKGVIVIVNKWDLAQEQRRAER
                     EDRPPRPEDEIKTAEEYRRIIAEGLKFIPYAPIIFTSAKTGYHVKSLLETALTISDNR
                     FLRVPTARLNEVVQEAKRHHNPTVFRGKVAKIYYATQTSVNPPTFVFFVNDPQALHFS
                     YERYLENQLRTAFGFKGTGIRLFFRARTQTERP"
     gene            complement(1456..>3009)
                     /locus_tag="VGT44_16405"
     CDS             complement(1456..>3009)
                     /locus_tag="VGT44_16405"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV2582440.1"
                     /translation="QQAEELSAAQEPTEPQELPELAELSASPELSTPEELEEPAEDQP
                     EQEQTDPPPDEALPAASSHVPDPPPLPITTRAHPSRQRRSRIPFVAALLILMCLVGVG
                     MLFSSHFFDTAASGGSGTGLGFVPALSPDAPIVQPPFTSAQMAEIMHLSGYMKDKALA
                     SLYVAHMTLDEELGQLIMVEYDSGCYTGTCSNATSYYSPDLDTMLNTLHAGGVIMYEF
                     QMQNFPQTKNDIAEMQRRAGIPLLIATDEEGGPYVHRLSHIYGYRMSATDIANTGDPN
                     IAAQQGAKAAQDLLSLGINVNLAPDVDVNLVNGYDMVTRTFGSTPQAVVKFASSYLEA
                     LQGDGVIGTIKHFPGLGDAVTDAHTTLPVVNRTRDQLYSVELAPFKAFIQTSNPLMYP
                     GIIMPTDVLMPAIDPVYPAELSHTFMTDILRNEFGYDGVVLTDALYMQGIIEKWDMNT
                     AAVLALNAGNDMLLGPTGAGQMLSMINALKAALANGTLSKARIDQAATRIITLKMEYH
                     LMPAAPPQE"
BASE COUNT          619 a          804 c          968 g          618 t
ORIGIN      
        1 tatgagcgaa gcagagcaag gaaaggggag aggggcgcaa atgaagccac ttgtcgccat
       61 cgtgggccga ccgaatgttg gcaaatccac gttttttaat cgcatgatcg gcgaacgggt
      121 cgccatcgtt gaagatatgc caggcacgac ccgtgatcgc atctatggcg acactgattg
      181 gaatgggcgc gagtttacgt tgattgatac gggcgggttg gagtttggct cggatattcc
      241 tatcgggcag gttggcctga acgggcagcc gggtgatttg atgaagcacg tgcaggccca
      301 ggccgaactg gccatcgaag aagccgatgt gatcgttttc atggttgacg cgcgtggagg
      361 tatcacggct gccgacgagg aggttgctga ggtgctgcgg cgcacgaata agccggtaat
      421 cctggcggcc aacaaggcgg ataatgcgcg gctgcgcctg gatgccgtcg agttttatgg
      481 gctgggaata ggcgagccta tcgacatctc ctcgttgcag ggcgttggca ccggcgacct
      541 gcttgatgct atcgtcgagg cactgccacc ggaagaagaa gagagcgacg gagaggacga
      601 ggagaccccg cgcatcgcca tcgtgggccg accgaatgtg ggcaaatcgt cgctgctcaa
      661 cgctatactt ggctacccgc gcgccattgt gagcgacata ccggggacga cgcgcgatgc
      721 catcgatacc gaactggaat ttgagggtcg aaaactgcgc ctgatcgata ccgccgggat
      781 acgcaggcgc gggcgcgtcg ggccgggcgt ggaaaagttc agcgtgctgc gttccagccg
      841 cgccatcgaa cgcgctgatg ttgccattct ggtgatagat gctggcgagg gcctggccgc
      901 gcaggacacg catatcgcgg gtgagattca agacctggcg aaaggcgtga tcgtcatcgt
      961 caacaaatgg gacctggcgc aggagcaacg ccgcgccgag cgtgaggata gacctccaag
     1021 accggaagat gagatcaaga ccgccgagga atatcgccgc atcattgccg agggactgaa
     1081 gttcatccct tatgcgccga tcatcttcac ctcggccaaa accggctatc acgtcaagtc
     1141 gctgctcgaa accgcgctca ccatctccga caaccgcttc ctgcgcgtgc cgacggcgcg
     1201 cctgaacgag gttgtgcagg aggctaaacg tcaccacaat ccgaccgtgt ttcgcggcaa
     1261 ggtggccaaa atctactatg cgacgcagac atcggtcaat ccgcccacgt tcgtcttttt
     1321 cgtcaacgat cctcaagccc tgcacttctc ctacgaacgc tacctggaga accagttgcg
     1381 caccgccttc ggcttcaagg ggacggggat acgcctgttc ttccgcgcaa gaacacagac
     1441 ggaacgtccg taggattatt cctgcggtgg cgcggcgggc atcaggtgat actccatttt
     1501 gagggtgatg atgcgcgtgg cggcctggtc gatgcgcgct ttggaaagcg tgccgttggc
     1561 gagggctgct ttcagcgcgt ttatcatgga gagcatctgg cccgcgccgg tggggccgag
     1621 cagcatatcg tttccggcgt tgagcgccag cactgccgcc gtgttcatgt cccacttttc
     1681 gataatgccc tgcatgtaga gcgcgtcggt cagcacaacc ccatcgtatc cgaactcgtt
     1741 gcgcaaaata tcggtcataa aggtgtgcga cagctcggcg ggatagacgg gatcgatagc
     1801 cggcatcagt acgtcggtcg gcatgatgat gccggggtac atcaaggggt tggatgtctg
     1861 gatgaaggct ttgaacggtg cgagttcgac ggaatagagc tgatccctgg tgcgattgac
     1921 gaccggcagg gtagtgtgcg cgtcggtgac ggcatcgcct aagccgggaa agtgtttgat
     1981 ggtgccgatc acgccatcgc cctgcaacgc ctcgaggtag gaactggcaa acttgacgac
     2041 tgcctgcggg gtgctgccga aggtgcgcgt caccatgtca taaccgttga cgaggttgac
     2101 atccacgtct ggggccaggt tgacattgat acccagcgag agcaagtcct gggcggcctt
     2161 cgcgccctgc tgtgcggcta tgttgggatc gccggtattg gctatatctg tcgctgacat
     2221 gcggtagcca tagatgtgtg agaggcgatg cacgtatggg ccgccttcct cgtcggtagc
     2281 gatgagcagt gggataccgg cgcgcctttg catctcggcg atatcgttct tcgtctgggg
     2341 gaagttctgc atctgaaatt cgtacataat cacgccgccc gcgtggaggg tgttaagcat
     2401 ggtatcgaga tcaggcgaat agtacgatgt ggcgttagaa cacgttcccg tgtagcaccc
     2461 ggagtcatat tccaccatga taagctggcc gagttcttca tcgagcgtca tgtgggccac
     2521 atacagactg gccagtgcct tgtccttcat atagccagaa agatgcatga tctctgccat
     2581 ttgcgctgat gtgaatggcg gctgtacgat gggcgcgtct ggcgagagcg cgggaacaaa
     2641 gcccaaaccc gtgccactac ccccgctcgc ggctgtatcg aagaaatgcg agctgaagag
     2701 catacccacc cctaccaggc acatcagaat gagcagtgcg gcgacgaatg gaatgcgcga
     2761 acgccgttgc ctggagggat gagcgcgtgt cgttataggt aacggcggtg gatcgggaac
     2821 atgtgaggac gcggcaggca aggcctcatc aggcggcggg tcggtttgct cctgttccgg
     2881 ctggtcttcg gcaggttcct cgagttcttc cggcgtagat aactctggcg aggcagataa
     2941 ttcagctaac tcaggtagtt cctgtggctc ggttggctct tgagctgctg atagctcttc
     3001 ggcctgctg
//
LOCUS       DASZAK010000935         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_592834, whole genome shotgun sequence.
ACCESSION   DASZAK010000935 DASZAK010000000
VERSION     DASZAK010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592834"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VGT44_16410"
     CDS             <1..749
                     /locus_tag="VGT44_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2582441.1"
                     /translation="SYVALACVLRQEFPRAEAALKAIMPREQQAANAAERHVAHAWGE
                     LSLAQGEPAVALSIAEQLIASAPGDMRQQPIPHLLALKGEALIALKRLEEAAAALESA
                     KLGAEQRQTPPVLWRIHRSLGQVYRLLKREDGAQAEWSATREIINRLAATVDQPYLRE
                     HFIQAALQTLPPEKPLPSRRAAAEKYDGLTEREIDVLRFVAQGLTDAQVAERLVISPR
                     TVHSHLNSIYSKLGITSRSAATRYAIEHDI"
     gene            916..1266
                     /locus_tag="VGT44_16415"
     CDS             916..1266
                     /locus_tag="VGT44_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2582442.1"
                     /translation="MSTSQVKSFNAPDETRTPPNAQMEFVKFGDMTIVRMTCKPGWRW
                     SEHMRPIAGTESCQGTHFTYIVSGHFRTVMDDGTVDDLGPGDIAITPPGHDAMVVGDE
                     PCVLFDFQGASRNV"
     gene            1298..>2298
                     /locus_tag="VGT44_16420"
     CDS             1298..>2298
                     /locus_tag="VGT44_16420"
                     /inference="COORDINATES: protein motif:HMM:NF044385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEV2582443.1"
                     /translation="MRVLITSIAAYGHLQPLLPLAKALADAGHEVAIATGPDLRLRAE
                     AAGFTAFNAGITFGTAFELLAERFPDEEYNRLAPAEILGWYLPHLFGETLAPAMLDDL
                     EPLVRNWQPDIVLHDTGEFAGPLAAASAGIPSVSQTPGLRTGDRLLNLVAVAVAALWR
                     QRGLDPDPSAGLYRHLCLDTTPPGFQPDESSRHHDVIRPLRPVALPPLPGERLPQWIN
                     ERQRGAPLVHMTLGTITNSNIAVFRSVIDGLSGLDVDVLITTGFGSDPAAFGPLASNI
                     HVERYVPQSLLLPHCAAVICHGGAGTTLSALALGLPLLILPQGADQYIIGDLVLAAGA
                     "
BASE COUNT          457 a          774 c          667 g          400 t
ORIGIN      
        1 cctcgtacgt agcgctggcc tgcgtactca gacaagagtt tcctcgcgcg gaagcagccc
       61 tcaaggccat catgccacgc gagcagcaag cggccaacgc ggcagaacga cacgtggccc
      121 acgcctgggg cgagctttca ctggcgcaag gagagccagc cgttgcgtta tccatcgcgg
      181 aacaattgat tgcatccgct cccggtgata tgcgccagca gcccatcccg cacctgctgg
      241 cattgaaagg cgaagccctg atcgccttga aacgcctgga ggaagcagcc gcagcattag
      301 agagcgctaa actcggcgca gagcagcgtc agacccctcc ggtcctctgg cgtatccacc
      361 gctcattggg gcaggtctac cgcctgctca aacgagagga cggggcgcag gctgagtgga
      421 gcgccaccag ggaaatcatc aaccggctgg ccgcaaccgt cgatcagccc tacctgcgcg
      481 agcatttcat ccaggcagcc ctgcaaacgc ttcccccgga aaagcccctg ccatcgcgcc
      541 gggccgcggc tgagaaatat gacgggttaa ccgaacgcga aatcgacgtg ctgcgtttcg
      601 tagcccaggg gctgaccgat gcccaggtgg cagaacgctt agtcatcagc ccgcgcaccg
      661 ttcattcaca cctcaactcg atctacagca agctcggcat tacgtcacgc agcgccgcca
      721 cgcgctacgc catcgaacac gacatatagg taattctgcc caggcgtcct caaacttcac
      781 cagaaaagac cgaaattcgc tcgaagagaa atgcgctatt cagccgatgt gtttggcccc
      841 tccggcaggc tatactcctg gcaatggttc tccgcttgtt cgagaaccga tagagaattg
      901 aaaagaggga ccaacatgtc aacatcgcaa gtgaagagct tcaatgctcc agacgaaacg
      961 cgcacccccc caaacgctca gatggaattc gtcaaatttg gcgatatgac catcgtaaga
     1021 atgacctgta agccgggctg gagatggtca gagcatatgc ggcccatcgc gggcacggag
     1081 agttgccagg gaacgcattt cacctacatc gtctctggac actttcgcac ggtgatggac
     1141 gacggcaccg tagatgatct ggggccgggt gatatcgcga ttaccccgcc aggacatgat
     1201 gccatggtcg tcggcgatga gccatgcgtt cttttcgact ttcaaggcgc gagcaggaat
     1261 gtctaagcaa gctggcgggt gacaaggaga tcaaagcatg cgagtactca ttacatctat
     1321 cgccgcatac ggccatctcc agccgctcct gccgctcgcg aaggcgctcg cggacgcggg
     1381 ccacgaggtg gccatagcca ccggccccga tctgcgcctg cgcgccgagg ctgccggctt
     1441 tacggccttt aacgccggga ttaccttcgg caccgccttc gagttgctgg cagagcgctt
     1501 ccccgatgag gagtacaacc ggctcgcgcc agccgagatc ctcggctggt acctgcctca
     1561 cctcttcggc gagaccctgg cacccgccat gcttgacgat ctggagccgc tggtgcggaa
     1621 ctggcagccg gatattgtgc tgcatgatac cggggagttc gccggtccgc tcgccgcggc
     1681 gagcgccggc atccccagcg tcagccagac gcccggactc cgcaccggcg accggcttct
     1741 caacctggtg gctgtggccg tcgccgcgct ctggcgacag cgcggcctgg accctgaccc
     1801 gtcggcaggt ctctatcgcc acctctgcct ggataccacg ccgcccggct tccagcccga
     1861 cgaatcatcg cgtcaccacg atgtgatccg tccgctgcgc cctgtcgccc tgccgccgct
     1921 ccccggcgag cgcctgccgc agtggatcaa cgagcgccaa cgaggggcgc cgctggtaca
     1981 tatgaccctc ggcacgatca ccaactctaa cattgcggtc ttccgaagcg tgatcgacgg
     2041 gctgagcggc ctggatgtcg atgtgctgat cacgaccggg ttcggcagcg atcccgccgc
     2101 ttttgggccg ttagccagca acatccacgt ggaacgctac gtccctcaat cgctgctcct
     2161 gccacactgc gcggcggtga tctgccacgg cggcgcggga accacgctca gcgcgctggc
     2221 gctcggtctg cctctgctga tcctccccca gggcgcggac caatacatca tcggcgacct
     2281 cgtcctcgcg gctggagc
//
LOCUS       DASZAK010000936         2693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_129379, whole genome shotgun sequence.
ACCESSION   DASZAK010000936 DASZAK010000000
VERSION     DASZAK010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129379"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VGT44_16425"
     CDS             complement(<1..206)
                     /locus_tag="VGT44_16425"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2582444.1"
                     /translation="MINLLRGPKRFNELRDLMGHISSKTLTQRLRALEEMGFVQRLAF
                     LEIPPRVEYHLTEKGQEFGHVIAA"
     gene            205..420
                     /locus_tag="VGT44_16430"
     CDS             205..420
                     /locus_tag="VGT44_16430"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV2582445.1"
                     /translation="MISRIHASPIKRMACPPLMVGLTLAGHGVQKLFGWFAGPGLNRL
                     KQGFRPAWLWVILAIVGEVEGIGKVFW"
     gene            complement(489..722)
                     /locus_tag="VGT44_16435"
     CDS             complement(489..722)
                     /locus_tag="VGT44_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582446.1"
                     /translation="MEEQNIYPTGSPVQVTSYSPFRGLNGTIQAVDTISDDIEEPFCF
                     YLVALEGTQLKEPMWFEYDEVESITSPLVALEA"
     gene            complement(1049..2182)
                     /locus_tag="VGT44_16440"
     CDS             complement(1049..2182)
                     /locus_tag="VGT44_16440"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV2582447.1"
                     /translation="MSILAVIFGSVLILLVALDAFENIVLPRRVTRRLRLSRLFYRVI
                     RLGWTSLGHLMRAGARRDTFLGYMGPLSLLALLAFWAVLFVFGFGLLLWGLALPLGAI
                     DKPISFLTYLYLSGTTFFTLGLGDVTPLAGAARLLVVIEVALGFIFLALVISYVPIIY
                     QAFSRRELRISLLDARAGSPPTAAELLRRNHAGRSVEELRLLLHDWEVWCADILESHL
                     SYPILAFYRSQHEQQSWVEALTVILDTCALILTGVEGTPVEPARFAFAMARHAVVDLA
                     QVLGTPPTPGVSRLSSSDFVHLQDVLAASGIHLKEGMATEQKLAEFRETYEPFVSALA
                     TRIQVALPPWLPQQDSLDDWQTSAWDDWFPSTRRTLLKVMHRE"
BASE COUNT          653 a          734 c          737 g          569 t
ORIGIN      
        1 atggctgcaa tcacatgccc aaattcctgc cctttttcgg tgagatgata ctcgacgcga
       61 ggtgggatct ccaggaaggc cagccgctgc acaaatccca tctcttccag ggccctgagc
      121 cgttgcgtga gcgtcttcga actgatatgt cccatcaaat ctcgcagttc gttaaagcgt
      181 tttggaccgc gcaacaggtt aatcatgatc agcagaatcc acgcatctcc aatcaaacga
      241 atggcatgtc cccccctgat ggttggcctg acgctggccg ggcatggagt gcaaaaactc
      301 tttggttggt tcgctggtcc tggcctcaac cggttgaaac agggcttcag gccggcatgg
      361 ttatgggtca tcctggcaat tgtcggggaa gtcgagggca ttgggaaagt gttctggtaa
      421 gggaggaaga aaaaatgggt atcccatgtg agatacccaa cttcacctgc ttcgtttata
      481 cacgaggctt atgcctcaag agccacgagc ggagaggtaa tcgactctac ctcatcatat
      541 tcaaaccaca ttggctcctt gagctgtgtt ccctccagag caacgaggta aaaacaaaac
      601 ggctcctcaa tatcatcgga aatggtatca accgcttgaa ttgtcccatt gagtccccgg
      661 aaagggctgt aactggtcac ttgtaccgga ctgcctgttg gatagatgtt ctgctcctcc
      721 atacggcttg tctcctttct tggggtatct ttgcttgtct ttcttacagg ataccatagt
      781 gtatcacatc gcattccatt atggcaagca caaccaatac taaacaagca tgcattgttt
      841 ctctaaaaat tccaccgatt tttctacaaa agtcggtaca atacaggcgt attgacgtgt
      901 cgcttcacag agggtaaacc gctacaataa tagcctcatg aacagcacag agagaaaatg
      961 taacctgttg agtctcgata tgtcgtaccg ccttctcagt gaaagtctcg ggaagtcgta
     1021 cctgtcaaga catgacatga ctactctttc attccctgtg catcaccttg aggagcgtcc
     1081 gacgtgttga aggaaaccaa tcatcccacg cgctggtttg ccaatcatcg agactgtcct
     1141 gctggggaag ccagggtggg agagcaacct ggatacgagt ggccagggca ctcacaaacg
     1201 gctcgtacgt ctcacgaaac tcggccagct tctgctcggt tgccattccc tccttcagat
     1261 ggatgccgga tgcggctagc acgtcctgga gatggacaaa gtcggaagat gacaggcgac
     1321 tcactcccgg cgtgggtggc gtcccaagga cctgcgccag gtcgacgacg gcgtgacggg
     1381 ccatagcaaa ggcaaatctg gctggttcta ccggtgtgcc ctcgacgcct gtcaagatca
     1441 gcgcgcacgt atccaggatc acggtcagcg cctccaccca ggattgctgt tcgtgttgcg
     1501 agcggtagaa agccaggata ggataggaca ggtgactctc caggatgtcg gcgcaccaca
     1561 cttcccagtc atgcaggagc aggcgcagtt cctcgacgct cctgccggca tgattgcggc
     1621 gcaggagttc cgctgccgtt ggtggagaac ctgcgcgggc gtccagcaag gagattcgga
     1681 gctcccgccg cgagaacgcc tggtagatga tggggacgta gctgatcacc agggccagga
     1741 agataaaccc caacgcgacc tcgatcacca ccagcagacg ggccgctccc gccagggggg
     1801 tgacatcacc caggccaagc gtgaaaaagg tggttccgct caggtacaga taggtcagga
     1861 agctgattgg tttgtcaatc gcgcccagcg gaagggccag gccccacagc agcagcccaa
     1921 atccgaagac gaagagcacc gcccagaacg ctaacagggc cagtaaggaa agcggcccca
     1981 tatagcctag aaaggtatcc cgtcttgcac ctgctcgcat caggtggccc agggatgtcc
     2041 accccagccg gatcacccga tagaaaagcc gggagagacg gaggcgtcgt gtcacccggc
     2101 ggggtaagac aatgttctca aaggcgtcca gggcaaccag caggatcagt acgctgccga
     2161 agatgaccgc aagtatgctc atcggttcct ccttgaaaga gaacagtgag ggagggtggc
     2221 gcttgtgcgc ttcaatagcc tcccagtgac tcctctttct gcaactgtag cagatttcct
     2281 cccgtttctc tatggcactg cgaacgatct gctcatgagc cagcgcttag ccacttcgag
     2341 cagtaggaag gatgtgctac tcattcgggc aaggaagagg aaagacaggg ctgttagcgg
     2401 cgtaaagccg agtggtgcga acgttcgtgt caccagagag catttcgcca gatacataca
     2461 aaagaagtgg atacgaaaac caaaggagtt cagtggcgtg ctgcaaccga actccgcatg
     2521 gaagactgcc gacaagactc tcttgatcgt cgctaggaca tgactacctt agccgtccca
     2581 tggacatgga atacctcgta gctttcacgt gcacctacct ggacggtatc gggacctgca
     2641 tgagtggctt ggatggcgag cgtcttaaac gattgttcat tctgaccacc cat
//
LOCUS       DASZAK010000937         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_343167, whole genome shotgun sequence.
ACCESSION   DASZAK010000937 DASZAK010000000
VERSION     DASZAK010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343167"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VGT44_16445"
     CDS             complement(<1..1068)
                     /locus_tag="VGT44_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV2582448.1"
                     /translation="MALFRQRGLRFPQGFLWGTASSSHQAEGGNTNNQWYRWEEAGRI
                     YTGEHSGDATNWWRAAEEDFALAEQMENNALRLSIEWSRVEPSEGVWDSAAIDRYRQM
                     LTNLRQRHMKPVVTLHHFTDPLWFVERGAFTRAENIRYFVRYATYVVERLSGLCDFWL
                     TFNEPNVYAYLGYLLGTFPPGEKNVLRGLQVLRNLVQAHVEGFYAINRVQPQAEIGYC
                     LNYRLMDPATPISPLDRFAANVQALFYNWGILQAGETGRFPFPMSALLASIPHAAGAR
                     DYHGVNYYTRDMVRFDITIPQEMFGRRFIRPGSVRNDASAGGDDTFGEIYPAGIYRVL
                     KNVYRRTRGNKPLYVTELGFND"
     gene            complement(1193..1789)
                     /locus_tag="VGT44_16450"
     CDS             complement(1193..1789)
                     /locus_tag="VGT44_16450"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEV2582449.1"
                     /translation="MEVQSPVFFASPSELRAWFEQNHETTRELLVGFYKTSSGKPSIT
                     WPESVDQALCFGWIDGVRKSIDDTSYTIRFTPRKPRSNWSSVNIKRIGELIELGLVQP
                     AGLKVFNERDQQKSGLYSYEQRKAIKLDPAYEEQLRANTKAWEYFQSRPPSYQQPAIL
                     WIMSAKQEETRLRRLATLIECSEQGRTISPLTRPTRTK"
     gene            2068..>2751
                     /locus_tag="VGT44_16455"
     CDS             2068..>2751
                     /locus_tag="VGT44_16455"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEV2582450.1"
                     /translation="MQENVSKYFKGFDAFVLSVMQDAGVPGLGLAVVKDTEVILEQGF
                     GKRNVAENLDVTPQTLFAIGSATKAFTAMALAILVKEGKLDWNVPVRHYLPTFRLHDP
                     VASERLTSRDLLIHNSGLPGYNAVWYNSTISRKELVNRLRHFEPTYDLRTTWQYQNMM
                     YMTAGYLVEMITGQTWEAFVQQRILEPLGMNSTNFSVHDSQQTPDFALPYKEIKDEVQ
                     AVKFYDRVQA"
BASE COUNT          590 a          727 c          756 g          678 t
ORIGIN      
        1 gtcgttaaag ccaagttcgg tgacgtacag gggtttgttg ccgcgtgttc gccgatagac
       61 atttttcagc acgcggtaga tgcccgccgg gtagatttcg ccaaaggtat catcgccgcc
      121 ggcgctggcg tcgttgcgca ccgatccggg gcggatgaaa cgtcgcccaa acatctcctg
      181 cggtatcgtg atatcgaagc gcaccatgtc gcgggtgtag tagttgacgc cgtgataatc
      241 gcgcgcgccc gccgcatgtg ggatggacgc cagcaacgcg ctcatcggga atgggaagcg
      301 gccggtttcg cccgcctgca agatgcccca gttgtagaac aatgcctgaa cgttagcggc
      361 gaagcgatct agtggcgaaa tgggagtagc tgggtccatg agacgatagt tcaggcagta
      421 gccgatctcc gcttgcggtt gaacgcggtt tatggcgtaa aagccctcaa catgtgcctg
      481 caccaggttg cgcagcacct gcaagccgcg cagcacgttc ttttcgcccg gcgggaacgt
      541 accgagcagg tagcccaggt aagcgtacac gttgggttcg ttgaaggtca accagaagtc
      601 gcacaggcca ctcaatcgct caacgacata ggtggcatag cgcacaaagt agcggatatt
      661 ctcggcgcga gtgaacgcgc cccgctcgac gaaccacagg ggatcggtga aatggtgcag
      721 cgtgacaact ggtttcatat gacgctggcg caggttggtc agcatctggc gatagcggtc
      781 aatcgccgcg ctatcccata cgccctcgga cggctcgacg cggctccatt cgatactcag
      841 ccgcagcgca ttgttctcca tctgttccgc cagggcgaaa tcttcttccg cagctcgcca
      901 ccagttcgta gcatcccccg aatgctcgcc ggtgtagatg cgccccgcct cttcccagcg
      961 ataccactga ttgttggtat tgccgccctc ggcctggtgc gatgaagacg cggttcccca
     1021 gagaaagccc tgtggaaagc gcaggccacg ctgcctgaac aatgccatac ggtgagactc
     1081 cttgccagaa tgattgatgc ttcgcttttg agtattgtat catactcgct tgcggaatcc
     1141 gttcattgaa aggttattct gccgcttttc gcctaccggt tatcaatatt tttcattttg
     1201 tcctggtcgg gcgcgttagc ggggaaatgg ttcgtccctg ctcagagcat tcgatgagcg
     1261 tagcaaggcg tcttagcctg gtttcttctt gtttggcgct catgatccat aagatagccg
     1321 gttgttgata tgatggtggc ctggattgga agtactccca cgcttttgta ttggctcgca
     1381 actgttcttc gtaagcggga tcgagtttga tagccttgcg ctgctcatac gagtagagtc
     1441 ccgatttttg ctggtcgcgt tcattgaaca ctttaagccc ggcaggctgc accagtccaa
     1501 gctcgatcag ttcaccaatg cgtttgatat tgacgctact ccagttactg cgcggcttgc
     1561 gcggtgtgaa ccggatggtg taactggtgt catcaatgct tttccgcacg ccatctatcc
     1621 agccaaaaca gagcgcctgg tctaccgatt ccggccaggt tatgctcggc ttccctgaac
     1681 tggttttgta aaacccaacc agcagttcgc gagttgtctc atggttctgc tcaaaccacg
     1741 cgcgcagttc agagggtgag gcaaagaaaa caggggattg tacttccatt tcgttcttcg
     1801 ctccaggcta tcgttattgc atactcttgt gattttagca cattcctggc cgcgaatagg
     1861 atatgtgaag cataggctat atccttatca ggattgtttt tccccctgaa atcgcgtatg
     1921 ctttctgtgg acccatctcg tctggaaagc attccctgtt cgaccgcccg gagctacgac
     1981 cgttgatgag gctggtgttt acgctaacga cagagtggtc ggtctactcc aaaacgctcc
     2041 tgacacatta gggaggatgt tatggccatg caagagaacg ttagtaaata cttcaagggt
     2101 tttgatgcat ttgtcctgtc cgtgatgcag gatgccggag taccgggatt ggggttagct
     2161 gtcgttaagg atacagaagt gattctggaa cagggctttg gcaagcgtaa tgtcgctgaa
     2221 aatttggatg tgactcctca aacgctgttt gctattggtt cggccaccaa ggcatttaca
     2281 gcaatggcgc tagcgatact ggttaaagag gggaaattag attggaatgt acccgtcaga
     2341 cattaccttc cgacatttcg actccacgat cctgtggcta gcgagcgtct gacctctcgc
     2401 gatctgctga tacataactc cggtctgcca ggctacaacg cggtttggta taactctacc
     2461 atctcgcgta aagagctggt gaatcgcctg cgacattttg aaccgacgta tgatctgcga
     2521 acgacctggc agtaccagaa catgatgtat atgaccgctg gctacctggt tgagatgatt
     2581 accggtcaga cctgggaagc gtttgtccag cagcgcatcc tggaaccgct aggtatgaat
     2641 agcaccaatt tctcggtcca cgactctcaa cagacacccg acttcgcgct gccctacaaa
     2701 gaaatcaagg atgaggtgca ggctgtgaaa ttctacgacc gcgtccaggc t
//
LOCUS       DASZAK010000938         1647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_592883, whole genome shotgun sequence.
ACCESSION   DASZAK010000938 DASZAK010000000
VERSION     DASZAK010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_592883"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..>1647)
                     /locus_tag="VGT44_16460"
     CDS             complement(24..>1647)
                     /locus_tag="VGT44_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582451.1"
                     /translation="SDQYALAVMVYEWLSGEVPFSGAPIEVALQHLNDPPPALSAVDI
                     PPAVEEVVLKALAKDPPARFASVQAFADALEAAAQGDAIHPGVLDAHIAVSSPQGTNK
                     QAKAGQEERESVAAARRPALLLAEREGRPRGPVGEFFWSRGSSSHGRILFLASLALIV
                     VIASFGVVLLVQPNPFSLTHSSPTPVSNVSTTIPQSFPTPTSIPTKVPPTSVPTLAPT
                     SKPLAPPAPPTLLLTVPSAVVGVAWSPGGRFYAAADVSGDVGVWQTPNKQQWEVNLGT
                     KIWSLAWSPDSPQAQRLAVAGDDGKVRIFDALSGKQLMLYGGHGAVSVLSVAWSKPYN
                     ATAVSVVSGDANGVIHVWDPATGNTLVTMNQTAPVDGLASAYSSLISAGAPPGGNVYM
                     WDTGSGQGSEQFNNSNSNIPYGADNPNLVEGTIVSAAWSADATYMALGDTNGNIQLIS
                     DTNCSCWMFQTAFLAHKGRVNSISWSSNNTTFATASDDGAIKTWRGADGSLVQTYRNP
                     YNAKDSAVAWSPDGQTLLAGDSMGRIMLWQVA"
BASE COUNT          340 a          506 c          479 g          322 t
ORIGIN      
        1 atgcctcacc tgggctgcct ctttcaggcc acctgccaga gcatgatgcg acccatactg
       61 tcccctgcca gcagcgtttg cccatccggc gaccaggcca ctgctgaatc tttggcattg
      121 tagggattgc gataggtctg aacaagcgat ccatcggccc cgcgccaggt cttgatggcg
      181 ccatcatcac tcgcggtagc gaatgtcgta ttattcgacg accaggagat ggagttgacg
      241 cgccccttat gcgcgagaaa agccgtctgg aacatccagc aactacaatt ggtgtcagaa
      301 atgagctgga tattcccgtt ggtatcgccg agcgccatat aggttgcatc agccgaccag
      361 gcagccgata caatcgtccc ctccacgaga ttcgggttat cagccccgta ggggatgtta
      421 ctattgctgt tgttaaattg ttccgaaccc tgccccgaac ctgtatccca catatagacg
      481 ttgcctcctg ggggcgcgcc tgctgagata agcgaagagt aagcgctggc aagcccatca
      541 accggggcgg tctgattcat ggtcacgagc gtattgccgg tggcagggtc ccagacatgt
      601 atcacgccat tcgcatcgcc ggagacgaca ctgacagcgg tagcgttata gggctttgac
      661 caggccacac tgagaacact caccgcgcca tgaccgccat agagcatgag ttgcttccca
      721 ctcaaagcgt cgaagatgcg cactttgcca tcgtcgccgg caacggcaag gcgctgcgct
      781 tgagggctgt cgggcgacca ggcgagtgac cagattttcg ttcccaggtt gacctcccac
      841 tgctgcttgt tgggcgtctg ccacacaccc acatctccgc tcacatccgc agcggcgtag
      901 aatcgtccgc cgggcgacca ggccacgccc accaccgccg aaggtactgt cagcaatagc
      961 gtaggaggcg caggaggcgc tagcggttta ctggttggcg ccagtgtagg gacgctggtc
     1021 ggcgggacct ttgtgggaat actggttggc gtagggaagc tttgaggaat agttgtagaa
     1081 acatttgata ccggtgtggg agaggaatgc gtcagtgaaa acgggttggg ctgcaccagc
     1141 aacacgacgc caaacgaggc aatcacaacg atcaacgcca ggctcgccag gaacaaaatg
     1201 cgcccatgag aggaactacc gcgcgaccag aagaactcac cgaccgggcc gcgtggcctg
     1261 ccctcacgct cggccaacag caacgctggc cgcctcgccg ctgctacaga ctctcgctct
     1321 tcttgccccg cctttgcctg tttattcgtt ccctgcggcg aagatactgc tatatgcgcg
     1381 tcgagaacgc cggggtggat agcgtcgccc tgcgctgccg cctccagcgc atcggcaaac
     1441 gcctgcacgc ttgcgaaacg cgcaggcgga tctttcgcca gcgctttgag taccacttcc
     1501 tccacggcgg gcgggatatc cacggcgctg agcgcaggcg gtggatcgtt caagtgttgc
     1561 agcgccacct cgattggcgc gccgctaaag ggtacttcgc cgctcagcca ttcgtagacc
     1621 atcacggcca gcgcgtactg atcgctg
//
LOCUS       DASZAK010000939         2628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_451676, whole genome shotgun sequence.
ACCESSION   DASZAK010000939 DASZAK010000000
VERSION     DASZAK010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451676"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VGT44_16465"
     CDS             <1..371
                     /locus_tag="VGT44_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582452.1"
                     /translation="CVRRPNAASKMARRAEWLIIGQMIGNLGTPPWKFTDDDLIGSKL
                     LGDVARFMVQVSGLKPSFLEAYLNLLRDWETERDSSEPPDIDLYTSVDNQTRQSEMQF
                     LNTEVEQLGSSELEANNTLG"
     gene            393..662
                     /locus_tag="VGT44_16470"
     CDS             393..662
                     /locus_tag="VGT44_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582453.1"
                     /translation="MHSSSDEQQPESTAPSPDTNLGASTPLADETSTLQEMSTQEGYA
                     LWAASYDQENNALIAIEEPHIDTLLARLPFTTTLDVGTPAQAAMR"
     gene            complement(911..2218)
                     /locus_tag="VGT44_16475"
     CDS             complement(911..2218)
                     /locus_tag="VGT44_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEV2582454.1"
                     /translation="MMGQVHPNILYIHSHDTGRYVQPYGHAIPTPNIQRLAESGVLFR
                     KAFSAAPTCSPSRASLLTGQYAHSNGMLGLAHRGFALHDYTQHLVHTLRKEGYRSALI
                     GEEHIAKDRNAIGYDYVAEIEGFQASIVAPAAVEMLASELTQPFFLSVGFFETHREFM
                     KPGSPTDARYAMPPIPLPDTPETRRDIGAFRASAWDLDKGMGAVLDALDNSGLADNTL
                     VICTTDHGIAFPRMKANLTDHGIGVMLIMRGPGGFSGGQVLDALVSHIDIFPTICDLL
                     QIDSPSWLQGTSMLPLIRGEVERVHDAVFAEVTYHAAYEPMRAVRTPRWKYIRRFDHH
                     LGPVLTNCDDSPSKDVLMAYGWKERSQPLEQLYDLVFDPNEAHTIADDLSVAVVLEEM
                     RARLDDWMRHTHDPLLHGPVPAPPGAELNDPDQLSPSSPTRFV"
     gene            complement(2236..>2628)
                     /locus_tag="VGT44_16480"
     CDS             complement(2236..>2628)
                     /locus_tag="VGT44_16480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582455.1"
                     /translation="LILVILEVGSYAFNWTWTGFKDNDTVWDWLQLLLLPIALAAVPI
                     WFAAEEAQQRIWMAQLRWVLVAALVVLAVLFVGTYAFNWTWTGFKDHGRVWDWLSLLL
                     VPVSVATLPIWYNIRQSHSSGMSQQAQE"
BASE COUNT          633 a          766 c          708 g          521 t
ORIGIN      
        1 gctgcgtgag gcgccctaat gcagcttcaa aaatggctcg tcgcgctgaa tggttgatta
       61 ttggtcagat gatcggcaat ctaggaactc ctccatggaa gtttactgat gatgatttga
      121 taggcagcaa gttgctgggt gatgtcgcaa gattcatggt acaagtgagt ggactcaaac
      181 cctccttttt ggaggcttac ctaaatttac ttcgagattg ggagactgag agggacagct
      241 cagagccacc agacatagat ttgtatactt cagtggacaa tcaaacgcgt cagtctgaaa
      301 tgcaatttct gaacacagaa gtcgagcaac ttggttcttc tgaactcgaa gctaacaata
      361 ctttaggttg attgaagaga cgaggcagta gtatgcattc ctcctctgat gaacaacaac
      421 cagaaagcac cgctccttct cctgacacga acctgggagc ctccacgccg ctcgccgacg
      481 aaacaagtac gcttcaagag atgagtaccc aggaaggata cgcgctctgg gccgcctcct
      541 acgaccagga aaacaatgcg ttaatcgcga tagaagagcc gcacatcgac acgctgctgg
      601 ccaggctgcc ttttaccacg acgctcgacg tggggacacc ggcacaggcc gctatgcgtt
      661 aaagcttgcc cggcgaggag caggcgtgac ggctatcgac cagtctgccg agatgctcgc
      721 ggtcgcccgg cgggcggctc agagcgaaaa cctgtccatc gatttccagt ttgcttcgct
      781 tgaaaaatgt agcgcagggc cctcaaggtc tcaccggcgc aaaaaagtgg atccaacaat
      841 tccaggtgga tgggcaagca atgtcattca cgcttcccca acgaaaaccc gatcctcaat
      901 agctcgctgc ctacacgaat cgagtcggag aggacggcga cagctggtcc gggtcattca
      961 gctccgctcc tggcggggca ggaacgggtc catgcagcag gggatcgtgc gtatggcgca
     1021 tccagtcgtc gaggcgggcg cgcatctctt ccagaacaac cgcgacggac aggtcatcgg
     1081 ctatagtatg cgcttcattg ggatcgaaga ccaggtcata caactgctct aacggctgtg
     1141 agcgttcctt ccaaccatac gccatcagca catccttgct cgggctatca tcgcagttcg
     1201 tcaggaccgg cccgaggtga tgatcgaaac ggcggatata cttccagcgt ggggtgcgta
     1261 ccgcgcgcat cggctcatat gcggcatgat acgttacctc cgcaaaaaca gcatcgtgga
     1321 cgcgctcgac ctcccctcga atcaagggca gcatcgaagt cccctgcaac caggagggag
     1381 aatcgatctg gagcaagtcg caaatggtcg ggaaaatatc gatgtgcgac accagcgcat
     1441 ccaacacctg gccgccgcta aagcctcccg gcccccgcat gatgagcatg acgccgatac
     1501 catgatccgt caggttcgct ttcatgcgcg gaaaagcaat gccgtgatcg gtcgtacaaa
     1561 tcaccagggt attgtcagca aggccgctgt tatccagcgc atccagcaca gcgcccatcc
     1621 ccttatccag gtcccaggcg ctcgccctaa acgcgccaat atcacggcgc gtctcaggcg
     1681 tatccggcag gggaatcggg ggcatcgcat acctggcatc ggtcggcgag ccgggcttca
     1741 tgaactcgcg gtgcgtttca aaaaagccaa cggagagaaa aaacggctgc gtcaactcac
     1801 ttgcaagcat ttcaacggcg gcaggggcca caatgctcgc ctggaaccct tcgatctccg
     1861 ccacatagtc gtagccaatc gcgttgcgat ctttcgcaat atgctcctcg ccgatcagcg
     1921 cggaacgata cccttctttg cgcagcgtat gcactagatg ctgcgtataa tcatgcagcg
     1981 caaagccgcg atgagccagc ccaagcatac cgttactgtg tgcgtactga cccgtgagca
     2041 ggctcgcgcg gctcggcgag caggtcggcg cggcgctgaa cgctttgcgg aacaaaacgc
     2101 cgctttcggc caggcgttgg atgttgggcg tcggaatggc gtggccataa ggctgcacgt
     2161 agcgaccggt atcatgtgag tgaatataga gaatgttagg gtgaacctgg cccatcatac
     2221 gcccccttca tcttgctact cttgcgcctg ctggctcatc ccgctgctat ggctttgtcg
     2281 gatgttgtac catattggca gggtagccac cgacaccggc accagcaaaa ggctcaacca
     2341 gtcccagacc ctcccgtgat ctttaaaacc ggtccaggtc cagttgaagg cataggttcc
     2401 tacgaacaac acagccagga ccaccagagc cgccacaagt acccacctca attgcgccat
     2461 ccagattcgt tgttgtgctt cctcggcagc gaaccagatc ggcacggcag ccagcgctat
     2521 cggaagcagc agcaattgca gccagtccca gaccgtatca ttgtccttaa agccagtcca
     2581 ggtccaattg aaagcatagg accctacttc caaaatcacc agaataag
//
LOCUS       DASZAK010000940         2268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_236241, whole genome shotgun sequence.
ACCESSION   DASZAK010000940 DASZAK010000000
VERSION     DASZAK010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236241"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VGT44_16485"
     CDS             complement(<1..461)
                     /locus_tag="VGT44_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582456.1"
                     /translation="MPKPFQFLLLAVGVLALIAVGGEITYSIVATTHGSTPVRVVQAQ
                     AGPYPLTVSLYTYPAHASYALPFAIAPQHPASGTLTYAVNSIPDPSDVPATPVRASIS
                     PDPNIHNGIQGDAEITVQGTWYLDITVTGPAGQGEALVPILATAPPAIPSWL"
     gene            complement(468..1649)
                     /locus_tag="VGT44_16490"
     CDS             complement(468..1649)
                     /locus_tag="VGT44_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582457.1"
                     /translation="MASTLDGTVVLDDSPTLARSLRSVATLRLVLGLTALLGAIVFLE
                     GTSWDIQWHSYIGRDRTLIPPHIMMLSGVALSGFAGLLTVLIESLWARRNKIIAQYGS
                     GFASIFSGPLGAYTVGFTALTAAVAFPLDAYWHSLYGIDVAIWAPFHIMFVASMGIVA
                     LGATYMLISAARLATRIGASANGARRAATLGAALALATLLSLFTLLLFDALDRRNLID
                     LGFMTLSVFPLLSGLLVAFTLVAAAYAIPWRWAATSVCAFYLLLAGIMAVYVQPATDW
                     LLHAENLQYRDPPSFTSIVALEWFITPLLVAILIDVFMRRARRKQWSQRKLTGFLTFT
                     ALLSGALPIVPILPIFPALLVLQLGVVGYLVSVLLGLAATYLGTFFGRNVGLSLDSLE
                     R"
     gene            1854..2045
                     /locus_tag="VGT44_16495"
     CDS             1854..2045
                     /locus_tag="VGT44_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582458.1"
                     /translation="MRSFHDHNRSGQAERSEASRCPSSQTLSAAKGGKRFPILVVNVH
                     DRVLMAVELGHPDAMMNFK"
BASE COUNT          509 a          546 c          797 g          416 t
ORIGIN      
        1 agccaggagg ggatggcggg tggggcggtg gccagaatgg ggacgagtgc ttctccctgg
       61 ccggcggggc cggtgacggt gatgtcgagg taccaggttc cctggacggt gatttccgcg
      121 tctccctgga tgccgttgtg gatattgggg tctgggctga tactggcgcg cacaggggtc
      181 gcgggtacgt cggagggatc agggattgag tttacggcgt aggtcagcgt cccgctggcg
      241 gggtgctggg gagcgatggc gaaggggagc gcgtagctgg cgtgggcagg gtaggtgtag
      301 agactgacgg tcaggggata ggggccagcc tgcgcctgta cgacgcgcac gggcgtgctg
      361 ccatgcgtgg tcgcgacgat tgaataagtg atttctccac caacggcgat cagggcaagc
      421 acgccgactg ccagcagcag aaattggaat ggctttggca tcttctttta cctttcgagc
      481 gagtcgagtg aaaggccaac gttccgaccg aaaaaggtac ccaggtaggt tgcggccagc
      541 cccagcagga cggagaccag gtagcctact acgcccaatt gcaggacgag caaagcggga
      601 aagatgggaa ggatggggac aatcggcaag gctccgctca gcagggcggt gaaggtcagg
      661 aagccggtta gctttcgctg ggaccactgt ttgcggcggg cgcggcgcat gaagacatcg
      721 atgagaatgg cgacaaggag cggagtgatg aaccattcaa gcgcgacgat cgaggtaaag
      781 gatggagggt cgcggtattg caaattttcc gcatggagca gccagtcggt ggcgggctgc
      841 acgtacacgg ccatgatgcc tgccagcaga aggtagaagg cgcaaacgga cgtggcagcc
      901 cagcgccagg gaatggcgta ggcggcggcc acgagcgtaa aggcgaccag caggccggac
      961 agcaatggga agacgctgag ggtcatgaag cccaggtcga ttagatttct ccggtcgagg
     1021 gcatcaaaga gcagcagcgt gaagaggctg aggagcgtgg caagggccag ggctgctccc
     1081 agcgtggcgg cgcgccgggc gccgttagct gacgcgccga tcctggtggc gagtcgcgcg
     1141 gcggatatca gcatgtaggt cgcgccgagc gcaacgatgc ccatgctggc gacaaacatg
     1201 atgtggaagg gagcccagat ggcgacgtcg atgccataga gggagtgcca gtaggcatcg
     1261 agcgggaaag cgacggcggc ggtgagggcg gtgaagccaa cggtgtaggc gccgagcggg
     1321 ccggagaaga tggatgcgaa gcctgagccg tattgggcga tgatcttatt gcggcgcgcc
     1381 cacaggcttt caatgaggac ggtgagcagg ccggcgaaac cactgagggc gacaccgctc
     1441 aacatcatga tatgcggcgg gatgagggtg cggtcacgcc cgatatacga gtgccattga
     1501 atatcccaac ttgtgccttc gaggaacacg attgcgccaa gcagggcggt cagtcccaac
     1561 accaggcgca aggtggctac ggagcgaagt gaacgcgcca gcgtgggtga gtcatcgagt
     1621 actactgtgc cgtcaagtgt actggccatg cgcgaaggtt cctttcgttt ttgtagatga
     1681 tatgcctatt gagttatgtg tatacatcgt tacaagcgga catgaggata tcgcgtagtc
     1741 tgtcaaagat cgattgcatg ttggcgatcg aaatcgctac atgatatacg aggggaatgc
     1801 aaaatggttg gaatttgatt ggatcgatat gcctttgccg ttgtagggat gcgatgagga
     1861 gttttcacga ccacaaccgg tccggtcaag ctgagcgcag cgaagcatct cgctgcccat
     1921 cgagccagac cctgagtgca gcgaagggtg gcaaacgctt cccgatcctg gtcgttaatg
     1981 ttcatgatcg cgtccttatg gcggtcgagc tgggccaccc ggatgcgatg atgaatttca
     2041 aataattaat ggggtacaac tgtaggtgcc gatttatcgc gcacatcgcc gataatgaac
     2101 tttaacaaat taatccggca ataagccaac gtagtgcagc aagtcgggag agggagactc
     2161 atactgagcc aaccagcgct cgacgcgcgc ttgcaagtcc ttccagcgac tgtgggagcg
     2221 atgcagatgc aacacttctt gcttgagctt gcgccagagc ttctcgat
//
LOCUS       DASZAK010000941         1751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_485469, whole genome shotgun sequence.
ACCESSION   DASZAK010000941 DASZAK010000000
VERSION     DASZAK010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485469"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VGT44_16500"
     CDS             complement(<1..216)
                     /locus_tag="VGT44_16500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582459.1"
                     /translation="MSQSLDKKRPRLSPRARVVIALAIIGLFVVLSFILPGKHEDASN
                     VTGDTVPSTAAVTNFVSSLTVNRSVDFN"
     gene            complement(273..1535)
                     /locus_tag="VGT44_16505"
     CDS             complement(273..1535)
                     /locus_tag="VGT44_16505"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEV2582460.1"
                     /translation="MKLTRRAMIKDGLLVVSAGMAMPAIFSRGVTSARAQALDGSRIA
                     QTASDRTLIIVQMAGGNDGLNTVIPFTDPLYIQMRPTLGIPGTKVLPLDTRLGLHPNL
                     QPLTKLWDEGHLAIVEGVGYPNQSLSHFQAMDIWQTLDLSGNGSDGWLGKLVAGLVDQ
                     EGHPFKALDIGVQTAQALQSISAQVPTVSSVKSYAIAPDPADTDRGNARLQALMKLYN
                     SYPGTAPYAALLDTTALNAQDGSRQLRQADATYRPAVTYPKGPFGDGLKVLAEAIVQG
                     LGLRVGYVTLGGFDTHANQQATHALLLQTLAGGLSAFYNDLAAHGKADNVVIMTWSEF
                     GRRVEENGSLGTDHGTAAPMFVLGNPVNKGIFGEPPSLSSLDGNGNLKYTVDFRSVYA
                     TVLDRWMGASSKDVLGGSFSSQNFLPQL"
     gene            complement(1554..>1751)
                     /locus_tag="VGT44_16510"
     CDS             complement(1554..>1751)
                     /locus_tag="VGT44_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582461.1"
                     /translation="DHFSSFLLDGNLDRDRRTRLLDYFTAQDDRGSGAPITLASGKSF
                     PLGRVRGTLYLMLASPEYQLN"
BASE COUNT          367 a          504 c          497 g          383 t
ORIGIN      
        1 gttgaagtcg acgctgcggt tcaccgtcaa gctactgaca aagttcgtta ctgccgcggt
       61 agagggcaca gtatcacccg ttacattgct cgcgtcttcg tgtttccccg gcaaaatgaa
      121 gctgagcacg acaaagagac ctatgattgc aagtgctatt acgacccgtg ctcttggtga
      181 caggcgcgga cgttttttat ccaacgattg tgacatgctt gctcaatact cccggttttt
      241 ctaaccagga tatattatag caaattcatg catcacagtt gcggcaggaa attttgtgaa
      301 ctaaacgacc cacccagcac atccttggag gacgcaccca tccagcggtc cagcaccgtg
      361 gcatagacgg agcggaaatc gacagtatat ttcaggttgc cgttcccgtc caggctgctc
      421 aggctcggcg gctcaccgaa aatgcccttg ttgacaggat tccccagcac gaacatggga
      481 gcggcggtgc catgatcggt acccaggctg ccgttctctt cgacacgccg cccaaactcc
      541 gaccaggtca tgatcaccac attatcggct ttgccgtggg ccgcgaggtc gttgtagaag
      601 gccgagagac caccggcgag cgtttgcaaa agcaaagcgt gcgttgcctg ctggttggca
      661 tgtgtgtcga agccgccgag cgtgacatag cccacccgca ggccgaggcc ctgcacaata
      721 gcctccgcta aaaccttgag gccgtcgcca aatggcccct tcggataggt cactgcaggc
      781 cggtacgtcg catccgcctg gcgcagctgg cgcgaaccgt cctgtgcgtt gagcgcggtt
      841 gtatccagca aagcggcata gggcgcagtt ccaggatagg agttgtagag cttcatcagc
      901 gcctgcaggc gtgcgttgcc gcgatcggta tcggcgggat cgggagcgat cgcatatgac
      961 ttgacactgg acaccgtcgg tacctgcgca ctgatcgatt gcagggcctg cgcggtttgt
     1021 acgccgatat cgagagcctt gaagggatgg ccttcctggt caaccagccc ggcaacgagt
     1081 ttacccagcc agccgtcgct gccattgccg ctcaggtcca gcgtttgcca gatgtccata
     1141 gcctggaagt gcgaaaggct ctggtttgga tagccgacac cctccacgat tgccaggtgc
     1201 ccctcatccc agagcttagt cagcggctgc aggtttggat gcagtcccag gcgtgtatcc
     1261 agtggcagca ctttcgtccc gggtatcccc agcgtggggc gcatttgtat atacaagggg
     1321 tcggtgaagg gtatgacggt gttgaggcca tcgttgccac ccgccatctg cacaatgatc
     1381 agcgtgcgat cactggctgt ctgagctata cgtgaaccat ccaatgcctg cgctcttgcc
     1441 gatgtaactc cacgactgaa gatggccggc atggccatac ccgctgacac gactaacaat
     1501 ccgtctttta tcattgctct gcgcgtgagt ttcatctgtc aaacctcttt cgtctagtta
     1561 agctgatatt caggtgatgc cagcatgaga tacagtgtgc cccgcacgcg acccagcgga
     1621 aaactcttcc cgctggccag ggtgatcggt gcgccgctac cgcggtcatc ttgagccgtg
     1681 aagtagtcga ggagacgtgt ccgacggtct ctatcgagat tgccgtccag taagaatgaa
     1741 gagaagtgat c
//
LOCUS       DASZAK010000942         2038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_24266, whole genome shotgun sequence.
ACCESSION   DASZAK010000942 DASZAK010000000
VERSION     DASZAK010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24266"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /gene="trbL"
                     /locus_tag="VGT44_16515"
     CDS             complement(<1..713)
                     /gene="trbL"
                     /locus_tag="VGT44_16515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbL"
                     /protein_id="HEV2582462.1"
                     /translation="MTAVALASVVTAHAQTPVDVGGTFQQIGQAANAWIPAIMHEAAF
                     LFYVLATLDFAWGAPSFLRESDFNGLFLSLIKKLLVISFFYAVLINGQVWIPAIVNSF
                     AQLGATAAGVPLAQNPSDIMMQGIQIVSDLFTKVSATDLLTQPGGAITTILAACIVLA
                     SYIIITLHYVVTKLEAIIVMSAGYIFLGFGGSRWTAPYFERYISLAVSTGVRLMLIYL
                     MLGVFKTVSNNWIATMNGYT"
     gene            complement(750..1070)
                     /locus_tag="VGT44_16520"
     CDS             complement(750..1070)
                     /locus_tag="VGT44_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582463.1"
                     /translation="MKRMSVWLAIAGLTMLAGCRHVEQSPIVAAFQQAGGGNVDQSTP
                     DSIGQFLAKHEDVRKQLTPLCKEKKANAPADWTTTDEGKVCAGNARANFFGKPDIKSD
                     GVAF"
     gene            complement(1084..1812)
                     /gene="trbJ"
                     /locus_tag="VGT44_16525"
     CDS             complement(1084..1812)
                     /gene="trbJ"
                     /locus_tag="VGT44_16525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009291 - unidirectional conjugation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HEV2582464.1"
                     /translation="MKSRWLAKGTSAVAMIAVVGTATICPPPAHAGVPGVFATEYTQL
                     LNYAELVGQLEKQVSMVENQLTQISDMTKHGLTISDQLFGTVASDIANLRQIVNTGQA
                     LSYTMSNMDGAFKVRFPGYSSTTNYGQSYQQWSQTSLDSTLGALKAAGLQNSQFDSDA
                     ALLKTLQNQSTSSVGRMQALEVGNQIAENQSEQLMKLRQLMMADMQSKAAFQGAQVQA
                     DATRQANSDKFFGQSQVTSDGVTF"
     gene            complement(1790..>2038)
                     /locus_tag="VGT44_16530"
     CDS             complement(1790..>2038)
                     /locus_tag="VGT44_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582465.1"
                     /translation="ATPKRDYYVVQRLGRRMISFRLGAVALAFLGVSGQRDRDRIDAL
                     AKEYGDGWISVWMKERKVEKSWIDYYEGVRQHEIQMAS"
BASE COUNT          431 a          585 c          523 g          499 t
ORIGIN      
        1 gtgtaaccgt tcatcgtcgc gatccaattg ttcgagaccg tcttgaacac ccccagcatg
       61 agatagatca gcatcaatcg cacgcctgtg ctgaccgcta gtgaaatgta cctttcaaaa
      121 taaggcgcag tccaacgact ccctccgaag ccaaggaaga tgtagcccgc agacatgaca
      181 atgatcgcct ccagcttcgt caccacatag tgaagcgtga tgatgatgta tgaagcgagg
      241 acgatacatg cggcgagaat cgtagttatt gcgccgccgg gttgcgtcaa taggtccgtc
      301 gcagacactt tcgtgaacag gtccgaaaca atctgaatcc cctgcatcat gatgtctgag
      361 ggattctgcg ccagcggcac acccgccgct gtcgccccga gctgggcaaa gctgttcaca
      421 atggcaggaa tccagacctg accattgatg agaactgcat agaagaacga aataacaagg
      481 agcttcttta tcaggctcag gaaaaggcca ttgaagtcgc tttcgcgtaa aaaggacggt
      541 gctccccagg cgaagtccaa agtcgccagc acgtagaaca ggaatgccgc ttcatgcatg
      601 atggcgggaa tccaagcatt tgccgcctgc cctatttgct ggaacgtccc acccacatcg
      661 acaggcgttt gtgcgtgtgc tgtgacgacc gaggcgagcg ccacggccgt cacaggcaag
      721 attctccgcg ttattgccat gccctcacct cagaaagcca caccgtctga cttgatatcc
      781 ggttttccga aaaagttggc gcgggcattg cccgcgcaca cctttccttc atcagtggtc
      841 gtccagtctg ccggggcatt cgccttcttc tctttgcaga gtggcgtgag ttgcttccgg
      901 acatcctcgt gcttggctag aaattgccca atgctgtcgg gcgtggactg atctacgttg
      961 ccgcctccag cttgttgaaa agccgcgacg atcgggcttt gctccacatg gcggcatccc
     1021 gccaacatcg tcagacccgc aattgccaac caaacactca tccgcttcat tgcaaatctc
     1081 cttttagaac gtcacgccat cgctggttac ctgactctgg ccgaagaact tgtcggagtt
     1141 tgcctgcctt gtcgcatccg cttgaacctg tgcgccctgg aaagctgctt tgctctgcat
     1201 atcggccatc atcagttgtc gcagcttcat cagctgctct gattgatttt ccgctatctg
     1261 gtttccaact tccagagctt gcatccttcc tactgaagac gtgctttggt tctgcaacgt
     1321 cttcagcagc gccgcgtcgc tgtcaaactg cgagttttgc agtccggcgg ctttcaaagc
     1381 cccgagagtg ctatcgaggc ttgtctggct ccactgctga tacgactgac catagttggt
     1441 agttgacgag tagccgggga atcgaacttt gaaagccccg tccatattgc tcattgtgta
     1501 ggagagtgcc tggcccgtat tcacgatctg tcgcaggttt gcgatatctg acgccactgt
     1561 tccgaagagt tggtcggaga tggtgaggcc gtgctttgtc atgtcggaaa tctgggtgag
     1621 ctggttttca accatcgaca cctgtttctc caactgtcca accagttccg cgtaattgag
     1681 gagctgggtg tactcggttg cgaatactcc cggtacccct gcatgggcag gaggcgggca
     1741 tattgtcgca gtccccacga cggcaatcat ggcgactgca cttgtccctt tagctagcca
     1801 tctggatttc atgttgtctt actccttcgt agtagtcgat ccagcttttc tctaccttcc
     1861 tttctttcat ccacaccgag atccagccgt cgccatactc tttggctagg gcgtcgattc
     1921 ggtctctgtc cctctggcca gagaccccta gaaatgcgag agccactgct ccgagacgga
     1981 aagagatcat gcgtcgtcca agacgctgga cgacgtagta atcacgcttg ggtgttgc
//
LOCUS       DASZAK010000943         2702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_451759, whole genome shotgun sequence.
ACCESSION   DASZAK010000943 DASZAK010000000
VERSION     DASZAK010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451759"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..566)
                     /locus_tag="VGT44_16535"
     CDS             complement(312..566)
                     /locus_tag="VGT44_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582466.1"
                     /translation="MKVCHCRSESAQLLLLVCHRLQELLILLLEGTHRVLLGVGEQMS
                     GMVQPDVGLSDRRPEMLRRPVALPGGSADWWCCGSDTRTR"
     gene            941..2131
                     /locus_tag="VGT44_16540"
     CDS             941..2131
                     /locus_tag="VGT44_16540"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEV2582467.1"
                     /translation="MHETLGVQYDVEALKRELDREELARRPAGIWRYRELLPVADPAF
                     RIDLGAGGTRLVPLTRIPEAASSRLLMKVEGANPSGSFKDRPIGVAACVALEQGARGL
                     ACLTSGNIGSALAAIAAKAGVPALVLLLGAAGLAGQGAPVNVEKYVQVSAYGASVITP
                     IGDLGQLYALADRVEAERGWSFMHNVQAFQTDGDKTTAFEICEQLGWQAPAAVFVPTG
                     TGTNLFGIWQGFVLFKELGFIDSLPRIFAVQPAGAASLVAAWQAHQSVPAALEHVEPN
                     LAPPISHRVSGYHAFKAIQESGGGAVAVSDEDMLAALKDLAAYEGIYTEVASAAALAG
                     IRHALAAGLLDPQEARQAGIVGILTSHGLKNSLALTSVFSPPPRVEGTWEALQSYLEL
                     KETE"
     gene            2137..>2702
                     /locus_tag="VGT44_16545"
     CDS             2137..>2702
                     /locus_tag="VGT44_16545"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbR-like 5'-deoxynucleotidase"
                     /protein_id="HEV2582468.1"
                     /translation="MDEHIDYEQIFTPQGMLGQVSKNPNLDFLLNIFNIPRVYSVSGF
                     GTWNVGQHTMAVAFLALYWSAFNDYPQEKRDRLVTLALVHDIHEAVIGDILPFFKTAA
                     VKEAIESIQNDILKAFSIEEDGSLHDELKLLDMIGFLYEIGQSSPRGIDLSKRKLIKL
                     MYARQKEQVLQYAEKAGIAQKTIGGFLT"
BASE COUNT          560 a          813 c          801 g          528 t
ORIGIN      
        1 atccgctttg tgctgctgca ccgagcagaa tttgaaacgc ttgccgctac agccggcttt
       61 caggtgctct ccttctatgg cgactatgcc tactccccct tccaggagga gagcagtccc
      121 tacatgatct gggtattggg aaaatagcca gaaagcgagc gttgccatgg ctcgcaaaca
      181 agcacggccc ccagccactc actccatcat ccttgaacca ctgcaaacca cctgccgcgc
      241 ctgtggcagt cggatgcgga tggcaagaca cagccatcgg acggtctcca cgttgcaagg
      301 ggtcacgcac ctcaccttgt gcgtgtatcg ctgccgcaac accaccagtc cgcgcttcca
      361 cccggcaacg cgaccggacg acggagcatc tctggccgcc gatccgacaa gcctacgtct
      421 ggttgcacca tgccgctcat ttgctcgcca acgccgagca gcacacggtg agtaccttcc
      481 agcagcagta tcaacaactc ctggagacga tgacacacca gcaggagaag ctgggcgctc
      541 tcgctccggc agtggcacac tttcacaaag tcactgaggt tccgccgcga tccgcagggg
      601 tatctgcttg cgttggagca gcgcctcctc accccgagtt tgccagccta gtttttttga
      661 aaagatgaat accgggccag ggcgatgctg gtcatcgccc tggccctgcc cgctggccat
      721 cgccctggcc cggtattcct accaaaatgc tatgattctt tcgatgcgca ttacgcacta
      781 cccaccatcg cgcatgagtg agatcgaccg ccggagcagg tcaggaggac gaagaattat
      841 gtacgcgaca acgttacgct gcacgttctg cggcgccgag tatcccttga gccattgcgg
      901 ttcctgcgcg tgctgcgcta ttccaggcga ggagagcgca ttgcatgaaa ctctcggcgt
      961 gcaatatgat gtggaggcgc tcaaacgcga actggatcgc gaggagttgg cgcgccgccc
     1021 ggcaggcata tggcgctatc gcgaactgtt gccggtcgcc gacccggcgt ttcgcatcga
     1081 cctgggagca ggtgggacgc ggctcgtccc gctcacacgc attcccgaag ctgccagcag
     1141 caggctcttg atgaaggtgg agggagctaa tccgagcggc tccttcaaag atcgcccgat
     1201 tggtgtcgcg gcctgtgtcg ccctggagca gggggcgcgc ggactggcct gcctgacctc
     1261 cggcaacatt ggctcggcgc tggcggccat cgctgcaaaa gcgggcgtcc ccgcgctcgt
     1321 cctgctgctc ggcgcggctg gactggcggg tcagggggcg ccggtcaacg tcgagaagta
     1381 cgtgcaggtc agcgcctacg gagccagcgt catcacgcct atcggcgacc tcggacagct
     1441 ttacgccctg gccgaccgcg tcgaggccga gcgaggctgg tcgttcatgc ataacgtgca
     1501 ggcgttccag accgacgggg ataagaccac cgcctttgag atctgcgagc agcttggttg
     1561 gcaggccccg gcagcggtct ttgtgccgac cgggacagga acgaatctat ttgggatatg
     1621 gcagggcttt gtgctcttca aggaactcgg gtttatcgat agcctgccgc gcatatttgc
     1681 cgtccagccg gcaggggccg cctcgcttgt cgccgcctgg caagcccacc agtcagttcc
     1741 cgccgcgttg gaacacgttg agccaaacct ggcgccgccc atcagtcacc gcgtcagcgg
     1801 atatcacgcc ttcaaagcca tacaggagtc gggtggaggc gctgtcgcgg tcagcgacga
     1861 agatatgctg gcggcattga aagacctcgc cgcgtatgag ggcatctata ccgaagtggc
     1921 gagcgcggcg gcgctggccg gaatacgaca tgccctggct gccggtctgc tcgacccgca
     1981 ggaggcacga caggccggga tcgttggcat actgaccagc cacgggttga agaatagcct
     2041 ggctctaacc tcggtattta gtcctccacc gagggtcgaa gggacatggg aggcattaca
     2101 aagttacctt gaattgaaag aaaccgagta aaagaaatgg atgaacacat tgattacgag
     2161 cagatcttta cccctcaggg gatgctcggc caggtatcca aaaatcccaa cctggatttc
     2221 ctcttgaata ttttcaacat ccccagggtg tacagcgttt ctggctttgg gacgtggaac
     2281 gtgggacaac acaccatggc ggtcgcgttc ctggcgctgt actggtccgc gtttaatgac
     2341 tatccgcagg agaaacgaga ccggctcgtg accctggcgc tcgtgcatga tattcacgag
     2401 gcagtgattg gggacatcct gccctttttc aagacggctg ccgtcaaaga ggccatcgag
     2461 tctatccaga acgatatcct gaaagccttc tcaatcgaag aagacgggtc gctgcacgat
     2521 gaactgaagc tgctcgacat gatcggcttc ctctatgaaa tcggccaatc ctcgcccagg
     2581 ggcattgacc tgtcgaagcg caaactgatc aagctgatgt atgcccggca gaaagagcag
     2641 gtcttgcagt atgccgagaa ggccgggatt gctcagaaaa cgataggtgg gtttctgaca
     2701 tt
//
LOCUS       DASZAK010000944         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_522653, whole genome shotgun sequence.
ACCESSION   DASZAK010000944 DASZAK010000000
VERSION     DASZAK010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522653"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VGT44_16550"
     CDS             <1..455
                     /locus_tag="VGT44_16550"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEV2582469.1"
                     /translation="TLAAPYGPAPVSEEAQQLTQQLFHLINHERAACGLPPFAWNNIL
                     ASGALLHSWNMAHCGFSHTCPDGSTPYQRIADEGFAGLSDCGESIGRAGPYPTAWDGV
                     YSVQESMAHEPLGGWHRIHLFSTTLHRIGIGVYVDAGGWIWFSEDMVS"
     gene            1055..>2234
                     /locus_tag="VGT44_16555"
     CDS             1055..>2234
                     /locus_tag="VGT44_16555"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582470.1"
                     /translation="MIDLAEYVDGLPNISGSEERIRQVIQASRTTPVFLPQGASGFGE
                     INSAFAIALHMHQPLIPAGGPELRTAQMISNLQWMLDNPDLDDNHNARVFLWCYKRMG
                     EFVPQLLDEGFQPRVMLEYSGTLLYGLRQMGATDVLDALARLTGDERNRRAVEWLGCP
                     WGHAVAPSTPVQDYRLHVEAWQHHFAAIFGLDALSRVRGFSPSEMALPNQPDVAYEFV
                     KTLIDCGYQWVLVGEHTIEQPDGQAPQHRHLPHRLVCTNSRGETATITAVIKTQGSDT
                     KLIGQMQPYEEAKGLSRLDLASKQIPPLVTQIADGENGGVMMNEFPAKYFEVIRACSA
                     SLTPIMNVTEYLERLQSVGVHENDLPVIQPLVQARIWERMVPGDGPHRLAEVINQLRA
                     E"
BASE COUNT          447 a          687 c          654 g          446 t
ORIGIN      
        1 ggacgctagc agctccctat ggaccggcac ctgtttcaga agaggcgcaa cagctcaccc
       61 agcaactctt ccacttgatt aatcatgaac gtgccgcctg tgggctcccc cctttcgcct
      121 ggaacaacat cctggcctcc ggtgcgcttc tgcatagctg gaacatggcg cactgcggct
      181 tcagccatac ctgccctgat ggcagcacgc cgtatcagcg catcgccgat gaaggcttcg
      241 ccggtctgag cgactgcgga gaaagtatcg gccgagcagg gccctatccg accgcctggg
      301 acggggttta ttctgtgcag gagagtatgg ctcacgagcc gctcgggggc tggcaccgta
      361 tccacctttt cagcacgacc ctgcatcgta tcggcatcgg cgtctatgtc gatgccggcg
      421 gatggatctg gttcagtgaa gatatggtaa gctaacaggt ccgttttcgc gaggtcatcc
      481 atccacccac cccaaaaata gctcatcgaa cggcatgcgc ccccctttgg ttttggaaca
      541 tgaggctgct cgcgactggg gagagggtag ccaagggaga tcacgacgcc aaagtggaga
      601 tcgtcgggaa cccctgggat cgctttggcc ttttccggct ccctgtgctg gctacttagg
      661 gcatcggttc gaaaaaatcg gcattttgtg catgattgat caagtttcct tgcccaagca
      721 tgtgagatgc gagtacaatg aacgagagcc atactgctct tacgcgtctt ccaacgcgcc
      781 tatttcacca aggagagggt caattcgtgc cttgtgtatt catctcaatg aagcaacgag
      841 tatgtttcac cacgtcgatc atcatgcgtt ggagtcggcc atgtctcgcg cgcttggact
      901 atggtattca tgccctgggc agtttcggca gttgatgatc aacggcatgc aacaggatca
      961 ctcgtgggcc agcgctgggc agcgctccct caacatctac gagtacatcc gtcataagtg
     1021 agagccggtc cagaccggcg cgaggagcgt aaccatgatt gaccttgcgg aatacgttga
     1081 tgggcttccc aacatctccg gctcagagga gcgcatcagg caggtgatcc aggctagccg
     1141 gaccacgccg gtgttcctgc cacagggggc ctcaggtttc ggtgagatca acagcgcctt
     1201 cgctattgca ttgcacatgc accagccact catcccggca gggggtcctg agctgcgaac
     1261 ggcacagatg atcagcaact tgcagtggat gctggacaat ccggacctcg atgacaacca
     1321 caacgcgcgc gtatttttgt ggtgttacaa acgcatgggc gagttcgtcc ctcaactcct
     1381 cgacgagggg ttccagccgc gggtcatgct cgaatactct ggcacgctgc tctacggatt
     1441 gcgccagatg ggcgccaccg atgtgctcga cgccctggcg cgcctcacag gtgacgagcg
     1501 caaccgccgg gcggtggagt ggctgggctg cccgtggggc cacgccgtgg ctccctccac
     1561 tccggtccag gactaccgcc ttcacgtcga ggcctggcag caccactttg ccgccatctt
     1621 tggccttgac gccttgagcc gggtgcgcgg cttctcgccc tcagaaatgg cgcttcccaa
     1681 ccagcccgac gtggcctacg agttcgtcaa gaccctcatc gactgcggct accagtgggt
     1741 cctcgtcggt gagcacacca tcgagcagcc ggatggacag gctccgcagc accgccatct
     1801 cccgcaccgc ctcgtctgca caaactctcg gggtgagacc gctaccatca cggctgtgat
     1861 caagacacag ggcagtgaca cgaagctgat cggccagatg cagccgtacg aggaggccaa
     1921 gggcctgtcc cggttggatc tcgccagcaa acagatcccc ccactggtga cccagattgc
     1981 cgatggggag aacggcgggg tgatgatgaa cgaattcccg gccaagtact tcgaggtgat
     2041 ccgcgcgtgc tccgcctctc ttacaccgat catgaacgtc accgaatacc tggagcggct
     2101 ccagtctgtg ggggtccatg aaaacgacct cccggtgatc caaccgctgg tccaagcacg
     2161 gatctgggag cgtatggtcc caggcgatgg accgcaccgg ctggctgagg tgatcaacca
     2221 gctgcgggcc gagg
//
LOCUS       DASZAK010000945         9351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_199637, whole genome shotgun sequence.
ACCESSION   DASZAK010000945 DASZAK010000000
VERSION     DASZAK010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9351
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199637"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1332
                     /locus_tag="VGT44_16560"
     CDS             130..1332
                     /locus_tag="VGT44_16560"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEV2582471.1"
                     /translation="MKTLTSNALARFAPTACLLVILVLLSACGGDNNAGSVTPTPRAT
                     STSGGGIGGSGTATPTLSATASIQLGQQPCPAPVKDPTHWDAIIPTQANVTSVDTVAC
                     GYLTGKASLQALVTVLHAGTGMVVDVYVYDNIAAPNPVQLFKLPGLYKGNAKISNYNT
                     VITAEVDSNSSINTGKPNALLEPDLFREFATGSFAPVSFPGFFPDLTRYQAELDQGQV
                     NQGQNAWELDPLQVASHFDSNQQMLNWTNFTSALVSGGGSGDQQAVVNVKNSGHAGAG
                     VTLTMQRLEGNTNGGIWEITAVATGGMAITAPQSRDTLTTPVTVSGSGTAFEGVIGPV
                     IVLDHTYSAIGHTTAMGTGNGPTTFSASVPYSSTFKTGKQDGILVLYATNNASGGYAG
                     AVMLKELL"
     gene            1464..3113
                     /locus_tag="VGT44_16565"
     CDS             1464..3113
                     /locus_tag="VGT44_16565"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEV2582472.1"
                     /translation="MPQESIDSILLAMSHRDEIDPDLLSIYFDRLDQEWTAGARDKVL
                     RLLRSKDISAHSAAVLILSELATNFDLEELEDLVADPTVSDMAKLTLAPILKELGSEM
                     ADAGLIEYLHDPESAMIQMQKHLLELIGQSELGIESVLEDVLGMPMEQRLGFISWLGM
                     SQDRRATKLLIPLLENQSSKVALAAIESLEQLGPEAAHQSIPALKYLLTVAGNKQVKQ
                     RARAALGRLTMQSVPGTEDAAMEQAFQTSQYPLYDARVSFVDGAGAQMILIAWQRPEG
                     LLKGVNVLYQDDWGIKDCYGSDDMDVDKWEELIERLKQEGLGSFQVPLAYCRALIVDA
                     RAVNRRTRHKLPVAYAVWRPSIEMEDETQKQIVETVLQPRPYTPDVAQLAMSGDTLYQ
                     LPEFNSWYYDPFEHLRNYVMRYSGVLSRIEAQTRERKRKTARLEASRADLETIVSEAL
                     EAVVDAPWRLRIESRLRRQAALFVLINRAEEAELLSAVASMLHPDSAVSPQQQPFLRA
                     MMHYTLENGLMRMLTEMIESGEGPFGKLPLGPFKGDDDWGL"
     gene            complement(3115..4419)
                     /locus_tag="VGT44_16570"
     CDS             complement(3115..4419)
                     /locus_tag="VGT44_16570"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEV2582473.1"
                     /translation="MADVQPLRGLRYSEVGNLADVLTPPYDVISEEAQARYYARHPYN
                     IIRLELGRDEQQDSALNNRYTRAAAALAEWRTQGVLHQENAAGYYVYQQAFAQGGQRY
                     TRTSLLARVRLEPWSARVILPHEHTLAKPKDDRLKLLEACAANLSPIMSLYDDPQGRM
                     RRLLAPYAANAGVQIVDEAQEEHRLQPITDAAQIELIQDFFSERRLYIADGHHRYETA
                     LKYREEILAMRKHLDPGDAVNFAMMALIDLDDPGLLVLPTHRLVRHLSQEALANLSNA
                     QLGRYFTVREMMMGDYPPETPLDMLAQVGEGAPAFVICTAEQGWLVSLNEQGKARMEQ
                     SGQSAAWNALDVAVAHTLALEALLGLNTDDMAAGTHVSYTRDARAALQAVRAGEAQAA
                     ILLNPTRVRQICDVAEANDRMPQKSTYFYPKLITGLVMNPLW"
     gene            4799..5095
                     /locus_tag="VGT44_16575"
     CDS             4799..5095
                     /locus_tag="VGT44_16575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582474.1"
                     /translation="MYPSIGQIVGFIAVVIVMSGLAIVLNRRIEKRRMAEHEARNQAW
                     VQLDVRRDADEEPSAEIESKTDDHDDERSNEINDQSLQTRAKQNGHYSESKRPG"
     gene            complement(5097..6200)
                     /locus_tag="VGT44_16580"
     CDS             complement(5097..6200)
                     /locus_tag="VGT44_16580"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV2582475.1"
                     /translation="MSDQQPTGLYRSIHAQPAAVRALLADWDGPTRAAEQLARARRII
                     VAGIGTSFHAATVGEYLLRLAGADAWAVRSFEFVHYPRPLRDDDAVIVVSHRGSKLHG
                     NLAVERALASGVTTIGITGRNSKMQGASPILETVEQDPSSTHSISYTGALTRLAQIAA
                     RLAALNGLTDQARALEHGLASIPGLMEGILSREDEVRQAASEAAAHNRRFYYIGAGPN
                     AVTATEGALKAKEAAYVTAEGFELEQGIHGPQVAFEGEDLLIPISVQGSAQARMADFL
                     LALGEIGSHVWLIGSAPSEETAALFDGARWKRFSLGNDADLSAVPEELTPMLAALPVQ
                     LLAEFLATARGTNADSFRADQEAYKRAGARFRI"
     gene            6592..7659
                     /locus_tag="VGT44_16585"
     CDS             6592..7659
                     /locus_tag="VGT44_16585"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV2582476.1"
                     /translation="MSVNMSSASNVVAAPQKRDLASMTFEERLQLGAENAVRCMGITA
                     KDRVIILTDFERESIARRVAIEAMARHADVTVRFLEHYGERPLTVFSEALRRDIMDVR
                     PTVSFYIATAKPGEITFRLPMLPFLVNELRVRHGHMIGISDELMAEGMCADYDEVFSL
                     TNQVYDLVRNAKTIHVTSVKGSDVTATFHKDWKWIPCHGRYGEQGMWGNLPEGEVFTA
                     PASVDGVLVCDVLGDFFSEKYGVLEHPVTIAVKGGYITGITSENEALAKELHDYFFST
                     PNGNRAGEFAIGTLTSLKRLVGNLLQDEKMPGLHVAFGNPYPEFTGADWHSKIHVDVI
                     PSVCTIEVDGRVIMRDGAFVI"
     gene            7895..>9351
                     /locus_tag="VGT44_16590"
     CDS             7895..>9351
                     /locus_tag="VGT44_16590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2582477.1"
                     /translation="MSTNRGQHLIGKEVGGCLLEQLIGYGGSSAVFLAQPHASDQKVA
                     VKVFLPHSTMDVSTQKSFYRRFLREAQAASELEHPNILSIYSYGEHQGLPYIVMPYVP
                     GGTLAEHVKERGQLTLREAQRYIQQIADALDYAHQQGCVHCDVKPANILLDQDGRVVL
                     SDFGIVRFMQGAVLNAQQSLKSSEILMGTPEYASPEQALGEALDGRSDIYSLAVTLFF
                     LLTGFPPFRSSTPIKILLMHVHDTPPKLSDMRGDVTARVDKVVEEALSKWPADRYQTA
                     GEFSRALSEAIFTAAQTRNIDRVTLTSAALKTLAENRKELEQSLQPVVEVKPLPRKPF
                     RITRSMAIVLVLGIVLLGSFTIGALFYTAASNHAYRPLTITPTSSANPDDLLSQHGMW
                     PSGPNGSAFFFSPQKYYIVNKTAQSTAIAVYGNHVYTNFRLTVTCSEVRSSSDGVDVY
                     GVVFRAKDDQSHYYIFGISPSGQYYFYRYNNGKYPT"
BASE COUNT         1969 a         2566 c         2702 g         2114 t
ORIGIN      
        1 ttccggctcg gcggatacga ccgtgcaaat ctggcaggaa tagatacaga gttcgtcacc
       61 atcatacttc ctctctgccg gtgatgtctg tatactgtat gtataccctt caaaatggag
      121 gttgatctta tgaaaactct gacttccaat gctctcgctc gtttcgctcc cactgcttgc
      181 ttgctcgtaa tcctcgtact gctgtctgcc tgtggagggg acaacaacgc tggcagcgtg
      241 acgccgacgc cgcgcgcgac atcgacatct ggaggtggca tcggcggtag tggaaccgcg
      301 acgccaacgc tttcggcgac ggcctctatc cagctcggcc agcagccctg tcccgcgccg
      361 gtgaaggatc cgactcattg ggatgccatc attccgacgc aggccaatgt cacttcggtt
      421 gatactgttg cgtgtggcta cctgaccggc aaagcatcgt tgcaggcgct tgtcaccgtc
      481 ctccacgcgg gaacgggtat ggtggtggat gtctacgtct atgacaatat cgccgcgccc
      541 aacccggtgc agttgttcaa attgcctggt ctgtataaag ggaacgcgaa gatcagcaat
      601 tataatacgg tgataactgc tgaggttgac tcgaattcga gcattaatac cggcaagccg
      661 aacgccctgc tcgaacctga cctcttccgg gaatttgcga ccggctcttt cgcgccagtc
      721 tcctttcctg gcttctttcc tgatctcacg cgctaccagg cggaattgga ccaggggcag
      781 gtgaatcagg gtcagaatgc ctgggaactt gatcccttac aggtcgcttc ccattttgac
      841 agcaatcagc agatgctgaa ctggacgaat ttcacgtccg cgcttgtcag cggaggtggt
      901 tcgggcgatc aacaggcggt ggtcaacgtc aaaaattctg gccacgctgg cgcgggcgtc
      961 acgcttacca tgcagcgcct ggaagggaat acaaacgggg gtatttggga aataaccgct
     1021 gtagcgaccg gcggtatggc gatcaccgcg ccgcaaagcc gcgacacact gacgacgcca
     1081 gtcaccgtga gcggttccgg tacggctttc gagggcgtaa ttggcccggt aatcgtcctc
     1141 gaccatacct atagcgctat cggccacacc acggcgatgg gaaccggaaa cggcccgacg
     1201 accttctcgg caagcgtacc ctatagctcc acctttaaga cggggaagca ggacggcatc
     1261 ctggtattgt acgccacaaa taatgccagt ggtggttatg ccggggcggt catgctgaaa
     1321 gaactgttgt agatggcaat agacgcggct tcttgaacgt gatacaatgc gtttcgttaa
     1381 accttctcag gaatatccag atagtagacg cacctcgatt tgctttgaaa ggctattgac
     1441 gaatgccaaa gagacacaag gagatgccgc aggaaagcat tgatagtata ttactggcaa
     1501 tgagccatcg tgatgaaatc gatcctgacc tcctttcgat atattttgat cgtctcgacc
     1561 aggaatggac tgccggggcg cgcgacaaag tgctgcgcct cttgcgctca aaagatatat
     1621 cggcccattc ggctgccgtg ctgatcctgt cagagctggc gaccaatttt gacctggaag
     1681 agctggaaga tctcgtcgcg gacccgaccg tcagcgatat ggccaagctg acgctagcgc
     1741 ctatactcaa agagcttggt tcggaaatgg ctgatgccgg cttgattgaa tacctgcacg
     1801 atcccgaatc ggccatgatt cagatgcaga agcacttgct cgaactgatt gggcagagcg
     1861 aactgggcat agagtcggtg ctggaagatg tgcttggtat gccgatggag cagcgcctgg
     1921 gcttcattag ctggctgggc atgagccagg atcgacgcgc gacaaaactg ctgatccccc
     1981 tgctggaaaa ccagtcgagt aaggtggcgc tggcggctat cgagtccctt gagcaattgg
     2041 gaccggaggc ggcgcatcag tcgattcctg ccttaaagta cttgctgaca gttgcaggca
     2101 acaagcaggt caagcagcgc gcgcgtgctg ccctggggcg gctgacgatg caatcggtgc
     2161 caggcacgga agatgccgcc atggagcaag cgttccagac gagccagtac cccctgtacg
     2221 atgcgcgcgt cagctttgtg gatggcgcgg gcgcgcagat gatcttgatt gcctggcagc
     2281 gcccagaggg actgctcaaa ggggtcaatg tcctctacca ggatgactgg ggcatcaagg
     2341 attgctatgg ctcggacgac atggatgtcg ataagtggga agagctgatc gagcgtttga
     2401 aacaggaggg gctgggtagt ttccaggttc ccctggccta ttgccgcgcc ctgattgtcg
     2461 atgcgcgcgc ggttaacagg cgcacacgtc ataaactgcc ggtggcctat gccgtctggc
     2521 ggccttcgat cgagatggaa gacgaaacgc agaaacagat tgtggagacg gttctccaac
     2581 cgcgccccta tacgccggat gtggctcagc tggctatgag tggcgatacg ctctaccaac
     2641 tgcctgaatt taactcgtgg tattatgatc cctttgaaca tctgcgaaat tacgtgatgc
     2701 gctatagtgg tgtcctcagc cgcatcgagg cgcagacgcg cgagcgcaag cgcaaaacgg
     2761 ccaggctgga agcgtccagg gccgacctgg aaacaattgt ttcggaggcg ctcgaagcgg
     2821 tcgtcgatgc cccctggcgg ttgcgcatag agtcgcgcct gcgccgccag gccgcgctct
     2881 ttgtcctcat caaccgcgcc gaagaggcgg aattgctgag cgcggttgca tcgatgctgc
     2941 acccggattc agccgtttcc ccgcagcagc aaccttttct ccgcgcaatg atgcattata
     3001 cgcttgaaaa cggcttgatg cgcatgctga cggagatgat cgagtcaggc gaggggccat
     3061 ttgggaagct cccgctaggt cccttcaaag gcgatgacga ctggggcctg taagtcacca
     3121 gagtgggttc atgaccagcc cggtgatcag tttcgggtaa aaataagtcg atttctgcgg
     3181 catacgatca ttcgcctcgg ctacgtcgca aatctggcgt acccgcgtgg gattgagcag
     3241 tattgccgcc tgcgcctcgc ccgctcgcac tgcctggaga gctgcgcgag cgtcgcgcgt
     3301 gtaactgacg tgtgtgcctg ccgccatgtc gtcggtgttc agtcccaaca gcgcttccaa
     3361 tgccagcgtg tgcgcgacag cgacatccag cgcgttccag gcagcgctct gcccgctctg
     3421 ctccatgcgc gccttgccct gctcgttcaa gctgaccagc cagccctgct ccgccgtgca
     3481 gatcacgaat gcaggcgcgc cttcgccgac ctgggccagc atatctaatg gcgtttctgg
     3541 cgggtaatcg cccatcatca tttcacgcac cgtgaaatat cgccccaatt gcgcgttgct
     3601 cagattcgcg agcgcctcct ggctgagatg gcgtaccagc cggtgcgtgg gcagcaccag
     3661 cagaccagga tcgtccaggt caatgagggc catcatcgcg aaatttaccg cgtcgccggg
     3721 gtctaaatgc ttgcgcattg ccagaatttc ttcgcgatac ttcagagccg tttcgtagcg
     3781 atgatgcccg tctgcaatat ataatcgtcg ctctgaaaag aagtcttgga taagttcaat
     3841 ttgcgcggca tcagtaatcg gttgcaggcg atgttcttcc tgcgcctcat cgacgatttg
     3901 caccccggcg ttcgcggcgt agggcgcgag caacctgcgc atacgcccct gtgggtcatc
     3961 gtagaggctc atgatcggac tcaaattggc ggcgcaggct tcgagcagct tcaggcggtc
     4021 gtccttcggt ttagccagcg tatgctcgtg cggcaggatc acgcgcgcgc tccacggctc
     4081 cagtcgcaca cgcgccagca ggctcgtgcg cgtatagcgc tggccgccct gggcgaatgc
     4141 ctgctggtag acgtagtagc cggcggcgtt ctcctgatga aggacaccct gcgtgcgcca
     4201 ttctgccagc gctgctgccg cgcgcgtgta gcgattgttc agcgcgctat cttgctgctc
     4261 gtcgcgtccc aactccagtc gaatgatgtt gtaggggtgg cgcgcgtagt aacgcgcctg
     4321 cgcctcttcg gagatgacat cgtagggtgg tgtaagcacg tcagccaggt tgccgacctc
     4381 ggaatagcgc aatccacgca atggttgtac gtctgccata cgactaactt tctcttgttt
     4441 tccaataacc ccgatagtca aaaatacgtc aaccatattg tagcatatgc taaaaaaatt
     4501 agatattatg ttctaaaata agcttgacaa cttttggagg agaaggtata atggactgtg
     4561 ttagaacaaa tgtttcacaa ctgaatatgc tctctcaagc ctctaatttt ctcctacttc
     4621 tcctcttgtg cggtcgggtg taatgctcct cttctccccc tcccgaccgc aggggctaaa
     4681 agaacaccgt ttctcccatg caggaaaaca cgcacttgac ggtaagcaat ctggccgtta
     4741 gaatgaatgc cgtacacagc gtctgaaact gtctcgtgtg tgaaaggtgc aagcatcgat
     4801 gtatccctct attggccaga tcgtcggctt tattgccgtg gttatcgtca tgagcgggct
     4861 ggcgatcgtg ctaaaccggc gtatcgaaaa gcgccggatg gccgagcatg aggctcgcaa
     4921 ccaggcgtgg gtgcaactcg atgtgcgccg cgatgctgat gaagagccat cggctgaaat
     4981 cgaaagtaag acagacgacc acgacgatga gcgctcaaac gagatcaatg accagagtct
     5041 gcaaacacgg gcaaagcaga atggacatta ctcggagagt aagcgtcctg gctagctcaa
     5101 atacggaacc gcgccccggc gcgtttgtat gcctcctggt cggcgcggaa actgtcggcg
     5161 ttggtgccgc gcgcggtggc caggaattcg gcgagcagtt gcacgggtag ggcggccagc
     5221 atgggcgtca gttcctccgg cacggcggag agatcggcgt cgtttcccag cgagaagcgt
     5281 ttccagcgcg cgccgtcaaa gagcgccgcc gtctcctctg acggggctga gccgatcagc
     5341 cagacatgcg agccgatctc gccgagcgcg agcagaaagt cggccatgcg cgcctgggcc
     5401 gacccttgca cactgatagg gatcaagagg tcttcgcctt caaaggccac ctgcggcccg
     5461 tgaatgccct gctccagttc aaagccttcg gctgtgacgt aggctgcttc cttcgctttg
     5521 agcgcgccct ccgtggcagt gacggcgttg ggacccgcgc cgatatagta gaagcggcgg
     5581 ttatgggctg ctgcctcgct tgctgcctgc ctcacttcat cctcgcggct caggatgcct
     5641 tccatcaggc cggggatgct tgccagacca tgttccaatg cgcgagcctg atctgtcaaa
     5701 ccattgagag cggccaggcg cgcggctatt tgagccaggc gtgtcagcgc gccggtgtag
     5761 ctgatcgaat gggtcgagga tgggtcttgc tcgacagtct caaggattgg actggcgccc
     5821 tgcatcttag aattgcgccc ggtgatgccg atggttgtca cgccgctggc cagggctcgc
     5881 tccaccgcca ggttgccgtg cagcttgctg ccccggtggc tcacgacgat caccgcgtca
     5941 tcgtctcgca ggggacgagg atagtgtacg aactcgaacg agcgcaccgc ccaggcatcg
     6001 gctccggcca gccgcagcag gtattctccg acggttgccg cgtgaaaact ggtgccgatc
     6061 ccagccacaa taatacgcct ggcccgcgcc aattgttccg ccgcccgcgt tggcccatcc
     6121 caatccgcca gcagcgcgcg aaccgccgcc ggctgcgcgt gaattgaacg gtatagcccg
     6181 gtaggttgtt gatctgacat gatgcgcgtc tcctttgcaa ataatggggg aacgtcccta
     6241 tggatagagt caatacgtgt agtatatgtg agatggtggc ttgtgcctac agccaagcgg
     6301 tgttgttacg ttcatccaag cctggatgaa ttgatgtgaa tattttgtaa cggtagaacg
     6361 ttctctacta tagaatgggt gtgttgttac ccggttaccg caaagttata tccctctccg
     6421 ggagcatgtg aattctcaca tagtaatctt tggggttgac cgttaccata gaaatgaagg
     6481 aacgttgttg ttgatgtcga attttattcg atgtagccgg ggtgggtgca agagcgatta
     6541 ctaccatcat cagcaatcat ctacatctgg acccttttga ggagaattgg tatgtctgta
     6601 aacatgagta gcgcgagcaa cgtagtggcc gctccccaaa agcgggatct cgcgagcatg
     6661 acatttgagg agcgcctgca attgggcgcg gaaaacgcgg ttcgttgtat gggcatcact
     6721 gccaaagacc gcgtgattat tcttactgac tttgagcggg agagtatcgc gcgccgtgtg
     6781 gcgattgagg cgatggctcg ccatgctgat gtgaccgtac gcttcctgga gcattatggc
     6841 gaacgccccc tgacagtctt ctccgaagcg ctgcgcaggg atattatgga tgtgcgtccg
     6901 accgtgagct tttatatcgc gacggcgaag cctggcgaga tcaccttccg cctgccgatg
     6961 ctgccattcc tggtgaatga actgcgcgtg cggcatgggc atatgatcgg cattagcgat
     7021 gagttgatgg ccgagggcat gtgcgccgat tacgacgaag tgtttagcct gacgaaccag
     7081 gtgtacgacc tggtgcgcaa cgcgaagacg atccatgtca cttccgtgaa gggcagcgat
     7141 gtgaccgcga ccttccacaa ggactggaaa tggattccct gccatgggcg ttacggcgaa
     7201 cagggtatgt ggggcaatct gcccgaaggc gaggtcttta ccgcgcccgc cagcgtggat
     7261 ggtgtgctgg tctgcgatgt actcggtgac ttcttctccg agaagtatgg tgtgcttgaa
     7321 cacccggtta cgattgccgt gaagggtggc tacatcacgg gcatcaccag tgagaatgag
     7381 gcgttggcga aggaattgca tgactacttc ttctctacgc cgaacggtaa tcgcgcgggc
     7441 gagtttgcca tcgggacgct taccagcctc aagcggctgg ttggcaacct gcttcaggac
     7501 gagaagatgc ctgggctgca cgtcgctttt ggcaacccgt atcctgaatt caccggcgcg
     7561 gactggcact caaaaatcca cgttgatgtc attcccagcg tttgcaccat cgaggtagat
     7621 ggtcgcgtta tcatgcgtga tggtgcattt gtgatataga ggcgttcatg cgtgtggcat
     7681 ggcaaccctt gcggttgcca tgccacacgt gcccgataaa tagccctggt gaaaactcct
     7741 ttaaatattg acattcctct ttgcttcttg atacaattgt aggggtcttt ttgatctacc
     7801 gcgtttcttt ctgtcccagg ctgtgacact aattgggttt gtcacgttcg tttgctcgct
     7861 caggggaatt attaagggga gctttacggt gtccatgtcc accaaccgag gacagcattt
     7921 gattgggaaa gaggttggcg ggtgccttct agagcagttg atcggctacg gagggtccag
     7981 cgccgtcttt ctcgcgcagc cgcacgcttc tgatcagaag gtggctgtga aggtgtttct
     8041 gccgcattca acaatggatg tttcgacaca gaaaagcttt taccgccgtt ttttacgcga
     8101 ggcgcaggca gccagcgaac tggagcatcc caacattctc tccatctatt cttacggcga
     8161 acatcagggt ttgccatata tcgtcatgcc gtatgtacct ggtggtacgc tggcggaaca
     8221 tgtcaaagag cgcggccagc tcacgctgcg ggaagcgcaa aggtacatcc agcagatcgc
     8281 ggacgcgctc gattacgcgc accagcaggg gtgtgtccac tgcgatgtca aaccggccaa
     8341 catcctgctc gatcaagatg ggcgggtggt cctttcggat ttcgggattg tgcgttttat
     8401 gcagggggca gtcctgaacg ctcagcagtc gctcaaatcc tcagagatat taatggggac
     8461 gcctgaatat gcttcgcctg aacaggcact gggcgaagcg cttgatgggc gttccgacat
     8521 ttattccctg gctgtaacgc tttttttctt actcaccgga tttccgcctt tccgctccag
     8581 cacgcccatt aagattctgc tgatgcatgt gcatgatacg ccacctaagc tcagcgatat
     8641 gcggggcgat gtaacagcac gtgtcgataa agtggtcgag gaggccttgt cgaaatggcc
     8701 tgcggaccgc tatcaaacgg caggtgaatt cagccgggca ttgagtgaag ccatcttcac
     8761 tgcggcgcaa acgcgcaata tcgaccgcgt cactctgaca tccgctgcgc tgaaaacact
     8821 ggcggagaac cgcaaggaac ttgaacagag cttgcagccg gttgttgagg tcaagcccct
     8881 gcccagaaag ccgttccgca ttactcgtag tatggcgatt gtgctggtgc tgggtattgt
     8941 tttacttgga tcgtttacga ttggggcctt gttttataca gccgccagca atcacgcgta
     9001 tcgccccctg acgattacgc caacatcctc ggccaaccca gatgacctgt tatctcaaca
     9061 tggcatgtgg cctagtgggc ccaatggcag cgcattcttt ttctcccctc aaaagtacta
     9121 catcgtaaac aaaactgcgc aatccaccgc aatcgccgtc tacggaaatc atgtctatac
     9181 taattttcgc ctgaccgtga cctgcagtga agtcagatca tcttcggacg gcgttgacgt
     9241 ttatggcgtc gtcttccgcg caaaggacga tcaatcgcat tactacatat tcggtatttc
     9301 gcccagcggg caatactact tttaccgcta taataatgga aaatatccca c
//
LOCUS       DASZAK010000946         2708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_129655, whole genome shotgun sequence.
ACCESSION   DASZAK010000946 DASZAK010000000
VERSION     DASZAK010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129655"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..478)
                     /locus_tag="VGT44_16595"
     CDS             complement(101..478)
                     /locus_tag="VGT44_16595"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2582478.1"
                     /translation="MSNHPIVHIEIPAVNAGEAGKFYNEVFGWKVNTEEMYGYVQFAA
                     EGGPAGGIVGLAAASADNPEYKPDSLIVYISTDDIDASLASVEAHGGKTLMAKHEIPH
                     VGWFALFSDPTGNRVALFTSVPH"
     gene            complement(654..1196)
                     /locus_tag="VGT44_16600"
     CDS             complement(654..1196)
                     /locus_tag="VGT44_16600"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEV2582479.1"
                     /translation="MAKVTTGEGDTGYTGLLGSQRVPKYDPRPDTFGTVDEATSALGL
                     ARAAATDPNVKQIIFQVQEELYLLMGELATPPENYEKMGLRMTVEHVRRLEETEEALK
                     REVEIPNKFIIPGDTLDGAALDLARTIIRRAERMAVKLLHDGVIQNGEVVRYLNRLSD
                     LIFILARFIEVKSSLAKLPE"
     gene            complement(1334..2209)
                     /locus_tag="VGT44_16605"
     CDS             complement(1334..2209)
                     /locus_tag="VGT44_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582480.1"
                     /translation="MNTPVSSSRAPILAQPARDATPRRLIVTAGVVIFAIRDWSLWAL
                     LRDGPWGPPMRLVLGTESVTDAVDRTIEAVLNWPEEATLLQLKAGWQEQQVYVWGSTE
                     EGAAVSLIHSVLLRANRDELRSDLHKHQVPSLRVGWKSVAQVEDGQHKLDAEVQPILE
                     TALNTLRSHVQRDPEIVLRYLADMGAMSEAAVQPDGSLWWHDGSVGNGHSRDTRPIDA
                     SRQPAAGDGILTLAEAALLYKAFFPPDEPIDLSNLRRRFLSSGKLQPLDEERPVRGKE
                     LDWRRVSRAYRYHIS"
     gene            complement(2350..2505)
                     /locus_tag="VGT44_16610"
     CDS             complement(2350..2505)
                     /locus_tag="VGT44_16610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582481.1"
                     /translation="MQNQTGYRRTSGAKAVAIDAREQLVQLRLLQLMAVILSAAFAWG
                     LHLIFVH"
BASE COUNT          563 a          747 c          794 g          604 t
ORIGIN      
        1 ttacatcgca cgacaaaaaa taggcgcgta ctctgctgct caatggtgct ttcgcagagt
       61 atgcgcctcc cggaaaggat cgccggacca ccgggtgtgg ctaatgcggt acgcttgtaa
      121 acagcgcgac gcgattgccg gtggggtcgc taaagagagc gaaccagccg acatggggaa
      181 tttcgtgttt ggccatgagc gtcttgccgc cgtgtgcctc gacgctggcg agcgaggcat
      241 cgatatcgtc ggtcgagatg tagacaatca ggctgtcggg tttatactcc gggttgtcag
      301 cagacgccgc agccagtccg acgatcccgc cagcgggacc gccttccgcc gcaaattgca
      361 cgtagccgta catttcctcg gtattgactt tccaaccaaa tacctcgttg tagaattttc
      421 ccgcctcgcc tgcattgaca gccggaattt caatgtggac gattggatgg tttgacatgg
      481 gataacctcc ttgtttttgt tccgaatgag agtagattca gccgtttgta gttgccaaag
      541 ctattataga atattagttc tattttttca aggattggaa gctggcgtag gactattgta
      601 cggatcgccg gtccctctat acatcatact gaaaggacat aatcgcgcgg tctctactcc
      661 ggcagcttcg ccagggatga tttgacctcg ataaagcggg ccagaatgaa gatcagatcg
      721 gagaggcgat tgaggtagcg cacaacctcg ccgttctgga tgacgccgtc gtgcagcagc
      781 ttgacggcca tgcgttcagc gcgacggatg atagtacgag caaggtcgag agcggcgcca
      841 tcgagggtat caccggggat gatgaatttg ttgggtatct cgacctcgcg tttgagggct
      901 tcttcggtct cctccaagcg gcgcacgtgt tctaccgtca tacgcagacc catcttttca
      961 tagttttccg gcggtgtagc cagttcgccc attagcagat agagttcctc ctgcacctgg
     1021 aagatgatct gcttgacgtt gggatcagtt gccgccgcgc gcgccagccc cagcgctgac
     1081 gtagcttcgt ccacggtgcc aaacgtatcc ggtcggggat cgtatttcgg cacgcgctgc
     1141 gagccaagca accctgtgta gcccgtatca ccttcgcctg ttgtgacttt agccatacat
     1201 attcctcctg aattgacgac cgggcaaccc tttgtaggga caggggcgcg gtgggtggcg
     1261 gggcgggggc cttgtgcctg tccgcgtttc cccgtcgatc atctctacca ggacaggcac
     1321 aaggcccctg tccctacgat atgtggtagc ggtaggcgcg gctgacacga cgccagtcga
     1381 gttctttgcc gcgcacgggg cgttcttcgt ccaggggctg gagtttgccg gaggacagga
     1441 aacgccgccg caggttggaa agatcgatag gttcatccgg cggaaaaaat gctttgtaga
     1501 gcagcgccgc ctcagccagt gtgagtatac catctccggc agcaggttgt cggctggcat
     1561 cgatggggcg cgtatctctg ctgtgtccat tgccaacgga accatcatgc caccataagc
     1621 tgccatccgg ctgcacagcc gcttcactca tcgcgcccat atcggccagg tagcgcagca
     1681 cgatttcagg gtcgcgctgg acgtgcgaac gcagagtatt gagcgcggtc tccagaatgg
     1741 gctgcacctc cgcgtcgagt ttgtgctgac catcttcgac ctgcgcgacc gatttccagc
     1801 cgacgcgcag gcttggcacc tggtgtttat gcaggtcaga gcgcaattcg tcgcggttgg
     1861 cgcgcagcag gacgctgtga atgagcgaga cagccgcgcc ttcttctgtg ctgccccaga
     1921 cgtagacctg ctgttcttgc cagcctgcct tgagttgcaa caatgtcgcc tcttccggcc
     1981 agttcagtac cgcctcgatc gtgcggtcaa cggcatccgt gaccgactct gtgccgagca
     2041 ccaggcgcat cggcggaccc caggggccgt cgcgcagcag cgcccagagc gaccagtcgc
     2101 gaatagcaaa aataacgact cctgcggtca cgatcaggcg gcgcggcgtt gcgtcgcgcg
     2161 caggctgggc caggatcggc gcacgtgaac tcgataccgg tgtgttcatg ctaacaagtt
     2221 ccctgcttcc acattcgata gttgggatat acggattgta cttgttctac tcgctgagcg
     2281 caagtcccca aaagaatcag aaggacacct ctgaacgagg tgtcctctat tttactgccg
     2341 cgaaagcgcc tagtggacaa aaatgagatg gagtccccag gcgaaagcgg cagagaggat
     2401 gactgccatg agttgaagta aacgcagctg tactagctgt tcgcgcgcat cgatagcaac
     2461 ggctttcgcg ccgctggtcc gcctataacc ggtctggttc tgcatagatg taccccttgc
     2521 tcctggatag gtagcagttt gtgaacgact tccatactat actacgtaaa ctatggagtc
     2581 gcggttgtat agacatattg taacgtataa ttgcggagat tgctatggga taggtcacac
     2641 atgaagagat gaagcaaagt cgagtgagca tggcgaccgc acgggtgcgc cactacgata
     2701 acgaacgg
//
LOCUS       DASZAK010000947         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_556106, whole genome shotgun sequence.
ACCESSION   DASZAK010000947 DASZAK010000000
VERSION     DASZAK010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556106"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(259..1605)
                     /locus_tag="VGT44_16615"
     CDS             complement(259..1605)
                     /locus_tag="VGT44_16615"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEV2582482.1"
                     /translation="MSLNRTVLQNGCVITLDKGLGDFRRADVLIEGSKIAAVGPDLAA
                     GDAEVLDASNMIVMPGFVDTHRHMWVDILRNIAVDLPFQGDVSYAAVVFNVLAPAFRP
                     QDVYAGNLVAALGAINAGITTVLDWSHIQVSPDYTDMAIKALQDSGMRAVFAYGTPWF
                     EPTKPEQPAWFRRTAERYFSSKDQLLTLGMAPLGPEFSSLDDVKTYWQLAREYDARIS
                     VHVGVGSSGQDGKLAEAGRAGLLGPDTTYVHCNTLSDDEIQMIVDTGGTVSLCTPVEM
                     IMGLGMPPAQRFLDRGLRPSLGVDAETTVPSDMFTQMRTLLPLQRMLIFDQGLAGKEN
                     LPPLLTARDVLEFATIEGARANGLAGKVGTLTPGKEADVILLRTDQINIVPVNDPVGA
                     VVWGMDTSNVDSVFVAGNALKRNGRLVNVDLDRVGKLAHESRDYVVETSGFKMPAF"
BASE COUNT          437 a          625 c          543 g          420 t
ORIGIN      
        1 ggacattcgc cataagcacc tcgcatctag gtagaagtgg taagagcaaa tgcgtagcga
       61 ccccgtcctc cgctttggca gaccttcacg ctcctcaatg ggcacatccg cttcacaaaa
      121 agtgggagcg ttccgctgtc agcccttgcg ccgcggcctt gccgatgccc atgctgccac
      181 cgcccacaag aacagcggta tatccttgca aacctggctc cctacatttg tgctcctttc
      241 agcaaagctc ttggccgctt aaaacgcggg cattttgaat cccgatgttt ccaccacata
      301 atcgcgcgat tcatgggcca gtttgccaac gcggtccagg tcgacattca ctagcctgcc
      361 attgcgcttc agggcattgc cggccacgaa aaccgagtcg acgttgctgg tatccatgcc
      421 ccacacgacc gcgccgacgg ggtcgttgac cggcacaatg ttgatctggt cggtgcgcag
      481 cagaatcaca tcggcctctt tgccgggagt gagcgtgccg actttaccgg ccaggccatt
      541 tgcccgcgcg ccctcgatcg tggcgaactc cagtacgtca cgggcggtca gcaggggcgg
      601 cagattctcc ttgcctgcca atccctgatc gaaaatgagc atgcgctgca agggcagcag
      661 cgtgcgcatc tgggtgaaca tatcgctcgg tacggtggtt tcagcatcga cacccaggct
      721 cggtcgcagg ccgcgatcaa ggaaacgctg cgcgggcggc atcccaaggc ccatgatcat
      781 ttcaacagga gtgcacagcg acacggtgcc acctgtgtcc acaatcatct gtatttcgtc
      841 atcactgagc gtgttgcagt gaacataggt cgtatctggc ccaagcaatc ccgctctgcc
      901 tgcctccgcc agtttgccat cctggccgga ggagccgacg ccaacatgca ccgagatgcg
      961 ggcatcgtac tcgcgggcaa gctgccagta cgtcttcaca tcgtcaagcg agctaaattc
     1021 cggccctaaa ggagccatac ccagcgtcag caactggtcc ttcgaggaga aatacctctc
     1081 ggcagttcgc ctgaaccatg cgggctgttc gggtttggtc ggctcaaacc agggcgtccc
     1141 gtaagcaaag acagcgcgca tccccgaatc ttgcaaagct ttgatagcca tgtcggtata
     1201 gtctggcgaa acttgtatat gggaccagtc gagaacggtg gttatgccgg cattgatggc
     1261 acccagggca gcgaccaggt tcccggcata cacatcttgc gggcggaaag ccggcgcgag
     1321 gacgttgaag accaccgccg cgtagctcac atcaccctga aacggaaggt ctacagcgat
     1381 gttgcgcagg atgtccaccc acatatggcg atgggtatcc acaaagcccg gcatcacgat
     1441 catgttcgag gcatcaagga cctcggcatc gcccgccgcg aggtctggcc cgaccgccgc
     1501 aattttcgac ccctcgatca gcacatcggc gcgccggaag tcgccgagtc ccttatcaag
     1561 agtgataacg cagccattct gtaacacagt tcgattgaga ctcataattt gccccctttg
     1621 acataggtgt cagctagtga cactgctgct ggcaatacca ctattgccgc tccattgaag
     1681 acattccaat acagaccccg tctctagcac tggtacctga tgcttttcca ctcgccctct
     1741 gcgagtttga gaaggatact ttcctctccc actgtaagga ggaagcgtca tgaaagcgtc
     1801 atgaaagcgt catgaacagc gtcagcggcc gtggcgtgag gaacggctct gctgcctctt
     1861 gcacacgtga gagccaggaa ggacgctctc acgcccgttc gacttctcag agcaaactct
     1921 tgggcatccc gtgtgatctg cccacgggat gcccaacgag ctgcactttt ggctaaagtg
     1981 gcttcacagg ctcgttcaat actctcgctt ttttctggca agaga
//
LOCUS       DASZAK010000948         1697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_166260, whole genome shotgun sequence.
ACCESSION   DASZAK010000948 DASZAK010000000
VERSION     DASZAK010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166260"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..718)
                     /locus_tag="VGT44_16620"
     CDS             complement(59..718)
                     /locus_tag="VGT44_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582483.1"
                     /translation="MPGRKEQEKYPWERGVSAGSDSLNGTGRQRPVPQRPPGLARVDT
                     PPAVPRVNRPQRQTKPPKSFRRRLLIFVGALAVLCVLIFAIVYGITNFVIGASSSAGA
                     ASTAGDFLSNLQIQNYSQAYQDLDATLTLNMKQLDFQQMATADDKCYGLVTDYTEVSG
                     SATTSTNGNTQSFTYTITRSKLSKTYTLTLTLGKDSNGSWDITNFGPDLGPAPPPATC
                     K"
     gene            complement(797..1486)
                     /locus_tag="VGT44_16625"
     CDS             complement(797..1486)
                     /locus_tag="VGT44_16625"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEV2582484.1"
                     /translation="MGKTSQEKHTLLTDYDTHSAYSEALHTLFANIRFQWEQESARSH
                     SLLITTPYAYGDYPALAANLAIVAAQSGAQTVLVDANMRAPVMQQRFGLEKTTGLSDL
                     LADTALTPEKVSSALQATFVPGLQLLGAGTQAEQGPALLLTSRLAEVIHSLCLPANDE
                     NGAEKTERIVLFHSSPVLAGADASLIGALVDQTALAIIANRTTRAQAKQAQEQLQRAR
                     AKLAGIVMVSM"
     gene            complement(1518..>1697)
                     /locus_tag="VGT44_16630"
     CDS             complement(1518..>1697)
                     /locus_tag="VGT44_16630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582485.1"
                     /translation="SSKLALYAALELVALAVGLALVFLLEYLDDRIREKDQAASLLGL
                     PILGSVPAAPLLRRR"
BASE COUNT          312 a          518 c          487 g          380 t
ORIGIN      
        1 gtgggctacc agaggttgcg cctctggtag cccacatacg ctcttttgta atggacatct
       61 atttgcaggt tgcaggcggc ggcgcgggac ccaggtctgg cccgaagttc gtgatatccc
      121 aactgccgtt gctgtctttc ccaagcgtta gcgtcagcgt gtatgttttc gaaagtttgc
      181 tacgtgttat cgtataggta aacgattgcg tattgccatt ggtggaagtg gtagcgctcc
      241 ccgacacctc ggtgtagtcg gtgacgagtc catagcattt atcgtccgcc gtcgccatct
      301 gctggaaatc caactgcttc atattcaagg tcagcgtggc atccaggtcc tggtatgcct
      361 ggctgtagtt ctggatttgc agattgctga gaaaatcgcc tgccgtgctt gccgcgcctg
      421 cgctggagct ggccccgatg acgaaattgg tgatcccgta gacgatggcg aagatcagga
      481 cgcagaggac cgccagcgcg ccaacaaata ttaacaggcg ccgccggaag cttttcggcg
      541 gtttggtctg ccgctgtggg cgattgaccc tgggtacggc gggcggcgta tcaactcgcg
      601 ccaggccggg cgggcgctgc ggtacgggac gctggcgtcc cgtgccattg agcgaatccg
      661 atcctgcgct cacaccacgc tcccagggat atttctcctg ctctttgcgt cctggcatca
      721 cttactctcc ttcaggacga ttatgcaact ccatcttcaa cccgaccaat cagtgcccct
      781 acgtcctggt atcatatcac atactcacca tcacgatacc cgcgagtttc gctcgcgccc
      841 gctgcaattg ttcctgcgcc tgctttgcct gcgcgcgcgt cgtccggttg gcgataatcg
      901 ccagtgccgt ctgatccacc agcgcaccaa tgagcgaggc atccgccccg gccagcacgg
      961 gtgagctatg aaaaagaacg atgcgctccg tcttttccgc gccgttctcg tcattcgctg
     1021 gcaagcaaag gctatgtatc acctctgcca ggcgagaagt aagcagcaag gcgggtccct
     1081 gttccgcctg cgtccccgcg ccgagcagtt gcagacccgg aacgaaggtt gcctgcagcg
     1141 cgctggagac cttctccggc gtgagagcag tatccgccag cagatcgctc aggccggtgg
     1201 ttttttccag gccgaagcgc tgctgcatca cgggggcgcg catgttcgcg tccaccagca
     1261 ccgtctgcgc gccgctctgt gccgccacga tggccaggtt cgcggccagc gccggatagt
     1321 ctccgtaggc gtagggggta gtgatgagca gcgaatgcga tcgagctgac tcttgctccc
     1381 actgaaagcg aatattggcg aacagcgtat ggagagcctc actatacgct gaatgtgtat
     1441 cgtaatcggt cagcaatgta tgcttttcct gtgaggtttt gcccacgatg ttgctccttc
     1501 tatcttcaat catgccctta tcgtcgcctc agcaacggcg cggctggcac gcttcccagg
     1561 atcggcagcc caagcaggct ggccgcctgg tccttctcgc gaatacggtc gtccaggtat
     1621 tccagcagga acaccagcgc gagacctacc gccagcgcga caagttccag ggccgcgtac
     1681 aatgccaatt tactgct
//
LOCUS       DASZAK010000949         4270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_166291, whole genome shotgun sequence.
ACCESSION   DASZAK010000949 DASZAK010000000
VERSION     DASZAK010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166291"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1344
                     /locus_tag="VGT44_16635"
     CDS             88..1344
                     /locus_tag="VGT44_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEV2582486.1"
                     /translation="MPGKGSITTTLTEDDVRSLLAQAYESLDADGKRVLVIIPDGTRS
                     LPMPLLFRLMYELWGQRVAQLDYLIALGTHPPMSEEAISHLVGVTREERARYYPDVHI
                     FNHAWNDPQALRTVGVISRAEAAALSDGLLVDEVQVTLNGRIFDYDRLALCGPVFPHE
                     VAGFSGGAKYLFPGIAGPDIIEFTHWLGALATSMRTIGVKDTAVRRVIQRAAQFVPLP
                     ILCIALVMQGDRLHGLYTGPQTEAWSAAADHSAQLNIISVPHPYTRVLSMPSLIYDDL
                     WTAAKAMYKTEPAIADGGEVIIYAPQITEISYTHGALIDRVGYHVRDFFLKQWPRFQD
                     IPGSILAHSTHVKGSGVYDADSGIESPRIQVTLATGIPEARCRQVNLGYLDYREIDPA
                     VWVNREDEGILFVPHAGEMLYRATGI"
     gene            1358..2425
                     /locus_tag="VGT44_16640"
     CDS             1358..2425
                     /locus_tag="VGT44_16640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2582487.1"
                     /translation="MTNPLRFGIVGCGVIGPVHAEAIASLPDAQLVAVTDLVLERAQK
                     LAERYGATPYTELAAMLERERLDVVIICTPSGKHGEHACQVMRSGRHVIVEKPMEVRR
                     EAINEMLRVQQETGVKLAVISQHRFDPASQQVRRLLDEQALGRLVLGHTIVPWWRSQA
                     YYDSGAWRGTWELDGGGVLMNQSIHSIDLLQWFMGPVKSVYAYSETLVHRMETEDVAV
                     AVLRFANGALGTIAATTGAYPGSATRIEVFGDKGSAILENDALAYLRLARDDKAEVGS
                     YGVQGGPKQPAAKQEQQPDVAQNPAALSSASHALQIADMIRAIRENGTPLVDGYAGSK
                     PVEIILSIYESARTRQEVFLS"
     gene            2422..3252
                     /locus_tag="VGT44_16645"
     CDS             2422..3252
                     /locus_tag="VGT44_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2582488.1"
                     /translation="MIRISAFADEISPDLDEQVAVLTRENIHYLDLRSAWNINVLDLN
                     DEQVKHIQQTLQEHHIEVAAIGSPIGKVPIDSPFEAEVQRLERAIAVAHAFHTPYIRI
                     FSFYPPATGGDVAAHRDEVMRRMRVMGERAREAAITLLHENEKDIYGDTIARCVDLMR
                     EDAGSGHVQTVFDPANFIQCGQTPFPDAYDALQPWLAYMHVKDALPDGRVVTAGEGSA
                     RWPALLQRLRVDGYEGFFALEPHLAAQGQLGGFSGPDLFHHAAQALRTMLQGMGWDYP
                     "
     gene            3269..4153
                     /locus_tag="VGT44_16650"
     CDS             3269..4153
                     /locus_tag="VGT44_16650"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2582489.1"
                     /translation="MSNATPRMFSLGDATIAIINAGDMMVDMAEELNAPESEWRPLYG
                     SVFEGSRPFPSQSVHIALPGASILVDANDYPLAIALEPSFLPPDYTPPPGIIEQLATI
                     GVRPADITHLVITHAHFDHYIGTTIEHDGKYLPRFPNAHVYLGRADWEDPQTQEELRN
                     PASIDSRTFGVLNRLGMLELVEGNRDLLPGVRIIAAPGESPGHQIVRVHLHGQTLYCL
                     GDLFHHPSEVEHPAWMVKWANASANLASRRALIEAALQEDAILIAAHMPPGRLEGTLS
                     NPRWTPLMETNNSMQPPS"
BASE COUNT          883 a         1357 c         1235 g          795 t
ORIGIN      
        1 tacaatgact ccgaagcgga tttacgtatc ggcctccgtc gtgtcattgt aagggcgtac
       61 ccttgcggtc gcccttctgg atattaaatg cccggcaaag gttctataac caccacccta
      121 accgaagacg acgttcgctc cctgctggcg caggcatatg aatccctgga cgccgatggg
      181 aagcgtgtgc tggttattat tcccgatggc acacgctcct tacccatgcc gctgctcttt
      241 cgcctgatgt acgagctgtg ggggcagcgt gttgcgcagc tcgattacct gattgcgctc
      301 ggcacacacc cgcctatgtc agaggaggcg atcagtcacc tggtaggcgt gacgcgggaa
      361 gaacgtgcgc ggtattaccc agatgtgcat atcttcaacc atgcctggaa cgatccgcag
      421 gcgctgcgca cagtcggcgt catctcgcgc gcggaggcag ccgcactctc ggatggcctg
      481 ctggtcgacg aggttcaggt cacgctcaac ggccggatat tcgactacga ccgccttgcg
      541 ctgtgcggtc ccgtctttcc ccacgaggtc gcgggcttct ccggcggggc gaaatacctg
      601 tttccgggta tcgccggacc agatatcatc gagttcacgc actggctcgg cgcgcttgcc
      661 acgagtatgc gcaccatcgg cgtcaaagat accgccgtgc gccgcgtcat tcagcgcgcg
      721 gcccagtttg tgcctctgcc tatcctctgc atcgcactgg tcatgcaagg cgaccggctg
      781 cacggcctct ataccgggcc gcagactgaa gcgtggagcg cggcggcaga ccactcggca
      841 cagttgaaca ttatcagcgt accccatccc tacacgcgcg tcttgtcgat gccctcgctc
      901 atatacgatg atctgtggac ggcggccaaa gccatgtaca aaacggaacc agccatcgcc
      961 gacggcggcg aagtcattat ctacgcgccg cagatcaccg aaatttccta cacacacggc
     1021 gcgctgatcg accgcgtggg ctatcacgtg cgtgactttt tccttaagca gtggccccgc
     1081 tttcaggata tacccggcag cattctggcg cacagtacac acgtgaaagg gtctggcgtg
     1141 tacgacgcgg acagcggtat cgaatcaccg cgcatccagg tgacgctggc gaccggcatt
     1201 cccgaagcgc gctgccgcca ggtcaacctg ggctatctcg actaccgcga gatcgacccg
     1261 gcggtatggg taaaccgtga agacgaaggg atcttgttcg taccacacgc gggcgagatg
     1321 ctctatcgcg cgacaggcat ctagacggag caaaaacatg acaaacccac tacgtttcgg
     1381 catcgtcgga tgcggcgtca tcggcccggt tcacgcggag gccatcgcaa gcctgcccga
     1441 cgcgcaactg gtggccgtca cagacctggt gctggaacgg gcgcaaaagc tggccgaacg
     1501 ctatggcgcg acgccctata ccgaactcgc ggccatgctg gaacgagagc ggctggatgt
     1561 cgtgattatc tgcacgccga gcggcaagca tggcgaacac gcctgccagg tgatgcgttc
     1621 aggacggcac gtcatcgtcg agaagccgat ggaggtgcgc cgtgaagcca tcaacgaaat
     1681 gctgcgcgtg cagcaagaaa ccggcgtcaa gctggcggtc atcagccagc accgcttcga
     1741 tcctgccagc cagcaggtac gccgcctgct ggacgagcag gcgctcggca ggctcgtgct
     1801 gggccacacc atcgtaccct ggtggcgctc gcaggcgtac tacgacagcg gcgcgtggcg
     1861 cggcacgtgg gagctggatg gtggcggtgt cttgatgaac cagtcgattc actcgatcga
     1921 tctcctgcaa tggttcatgg gtcccgtcaa aagcgtttat gcctatagcg agacgcttgt
     1981 tcaccgcatg gaaacagaag atgttgccgt tgccgtgctg cgtttcgcca acggggcgct
     2041 cggcaccatc gccgcgacaa cgggagcgta ccccggcagc gcaactcgca tcgaggtgtt
     2101 cggtgacaaa ggttcggcca tcctcgaaaa cgacgccctg gcctacctgc gcctggcgcg
     2161 cgacgacaaa gcggaagtgg gcagctacgg cgttcaaggc ggaccaaaac aacccgccgc
     2221 aaagcaagag cagcagcccg acgtggcgca gaatcccgcc gcgttgtcca gcgccagcca
     2281 tgccttgcag atcgccgaca tgattcgcgc catccgcgag aacggaacac cgctcgtaga
     2341 cggctacgcg ggcagcaaac cggtcgagat catcctgagc atctatgaat cggcgcgtac
     2401 acgacaggag gtcttcctat catgattcgc atctctgcct tcgccgacga aatctcgccc
     2461 gacctggacg aacaggtggc cgtcctcacg cgcgagaaca tccattacct ggacctgcgt
     2521 agcgcctgga acatcaatgt cctcgatctg aacgacgagc aggtaaagca tattcaacag
     2581 acattgcaag aacaccatat cgaggtcgcg gccatcggct cccccatcgg caaagtcccc
     2641 atcgatagcc cgtttgaggc cgaggtacaa cgcctggaac gcgctatcgc cgttgcgcac
     2701 gctttccata ccccgtatat ccgcatcttc tcgttctacc cgcccgcgac cggcggtgat
     2761 gtggcagcgc accgggacga ggtaatgcgc cggatgcgtg ttatgggcga acgcgcgcgc
     2821 gaagccgcta tcacactgct gcacgagaac gagaaggaca tctacggaga taccatcgcg
     2881 cgctgcgtcg atctgatgcg cgaggacgcc ggcagtggac atgtgcaaac ggtgttcgat
     2941 ccggcgaact tcattcaatg cgggcagacg ccctttcccg atgcatacga cgccttgcag
     3001 ccctggttgg cctacatgca cgtcaaagat gcgcttcccg atggccgcgt cgttacagcg
     3061 ggcgagggca gcgctcgctg gcccgcgctg ctgcaacgcc tgcgagtcga cggctacgaa
     3121 ggcttcttcg ccctcgagcc gcacctggcc gcgcaaggtc agcttggcgg ctttagcggg
     3181 cctgacctgt ttcatcacgc agcacaggca ttgcgcacaa tgctgcaagg catgggatgg
     3241 gactatccgt aaggagaaag gtccagttat gtcgaacgcc actccgcgca tgttctcgtt
     3301 aggtgatgcc accatcgcca tcatcaacgc cggtgacatg atggtcgata tggctgaaga
     3361 attgaacgct cctgagagcg agtggcgacc tctctatggc tcagtttttg aggggagcag
     3421 gccatttccc agccagagcg tgcatatcgc gcttccgggc gcatctatcc tggttgacgc
     3481 caacgactac cccctggcca ttgcgctgga gccatccttc ctgccacccg attatacgcc
     3541 gccgccaggc atcatcgaac aactcgcaac gattggcgtc cgccccgcag acatcaccca
     3601 cctggtgatt acccacgcac acttcgatca ctacataggt accaccatcg aacatgatgg
     3661 taagtactta ccacgtttcc ccaacgcgca tgtgtactta ggacgagctg attgggaaga
     3721 cccacaaacg caggaggagc tgcgcaaccc cgcttccatc gatagccgca ccttcggcgt
     3781 ccttaaccgc ctgggaatgc tcgaactggt cgaaggcaac cgcgacctgc tgcccggcgt
     3841 gcgtattatc gccgctcccg gtgaatctcc cggccaccag atcgtgcgcg tgcatttgca
     3901 cggacagacc ctctattgcc tgggcgacct cttccaccat ccctccgaag tggaacatcc
     3961 cgcctggatg gtcaaatggg ccaatgccag cgccaacctc gccagccgcc gcgccctgat
     4021 cgaggccgcg ctgcaagaag atgctattct gatagccgcg catatgcccc ctggccgatt
     4081 ggaagggaca ttgtcaaatc ccaggtggac gccattgatg gagaccaaca attcaatgca
     4141 gcccccgtcg tagggacgcg attgatcgtg tcctggtggc ccagttcgat caccggcgag
     4201 gacgcgatca tgaatgttta cgaccaaagt cgggaatgtc atcctgagcg cagcgacgga
     4261 tctgaattcg
//
LOCUS       DASZAK010000950         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_129759, whole genome shotgun sequence.
ACCESSION   DASZAK010000950 DASZAK010000000
VERSION     DASZAK010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129759"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..>2142)
                     /locus_tag="VGT44_16655"
     CDS             complement(44..>2142)
                     /locus_tag="VGT44_16655"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2582490.1"
                     /translation="TPDNTRILQTVLDELPGADQALQGHTASRLSPGPGGQDWLFSAV
                     PVPDAGWAVVVQRPAAEAQAVVAQFHLWLLTAALLFAIGGLLFWLMLLVRVIRPLHTL
                     AIQHQALPVSEQSIPVHTNVLAARNDEVGDLARSLVRLELDRLKKLGELRTLLETSNA
                     VVGSLEPQAVVGKIIGEVRRLVDVQAAAVLLPDEHGVLRVLASAGHSERYHNVLSLSP
                     ENVSSAPVLALRDGKPVQKLLGPEHPMPSLSYEEGFRSVLAIPIISRHVGGVILLVHR
                     TEPRPFNQNEINLLLTFANYATLAWEHAVLYERSDERLRAVAQENERLYQHASEEKQK
                     LEAIMSSMSDGLVLTGIDGMVLYANQGASTIVGLPSEALVGYPISTLYDALRARAVDP
                     ADCERILAQAESTETTELVVEIRRYDRHRAIHLRLFDVGDESGRAIGRGLLLRDVTRE
                     RELDEFKTTLLAAVGHEVRTPLAAIKGHASTLLQKDVTWPLADQRHFLQTISGEADRL
                     AQLVSNLLDLSRQEAGLLLLNRGPTRMQDLVVKIIEQLSHAGASISSHIPDDLPPVNV
                     DSARIEVVLHNLVANALVYGDGEVRITAERRDDVIVVSVSDNGPGIAPDELPHVFERF
                     YRARHGRQQHSGGTGLGLTICKAFIKAHGGVIWAESSVQGTTISFSMPLVSPTDAAVP
                     VDTRAKELSTEEWRFV"
BASE COUNT          427 a          655 c          638 g          422 t
ORIGIN      
        1 gcgacttcat cttcagcgag cagaatgcgc tttgccttcg tcgtcatacg aacctccatt
       61 cctctgtcga gagctccttt gcccttgtat caacaggcac agcggcatct gtaggtgaga
      121 ccagcggcat tgagaatgag atagtggtgc cctgcacgct gctttccgcc cagatgacgc
      181 ctccatgggc cttgatgaaa gctttgcaga tggtcagtcc gaggccagtg ccgccagaat
      241 gctgctgtcg gccatgtcgc gctcggtaga aacgctcgaa cacatggggc aactcgtcgg
      301 gagcgatgcc aggtccattg tccgaaactg atacgacaat cacatcgtcc cgcctctcgg
      361 cggtgatacg cacttcgcca tcaccgtaca ccagcgcatt ggcgaccaga ttgtggagca
      421 cgacctctat gcgtgcgctg tccacgttga ccggaggcag gtcgtcgggg atgtgcgaag
      481 atatgctggc acccgcatga ctcagctgct cgatgatctt cactacgaga tcctgcatcc
      541 ttgtaggtcc gcgattgagg aggagcagcc cagcttcctg gcgcgagagg tcgagcaggt
      601 tgcttacaag ctgcgccaag cgatcggctt cgccgctgat cgtctgcagg aaatggcgct
      661 ggtcagcaag cggccaggtt acgtctttct ggagcaacgt cgaggcgtgg cctttgattg
      721 ccgccagcgg ggtgcgcact tcatggccaa ccgccgccaa gagcgtcgtc ttgaattcgt
      781 caagctcgcg ctcgcgggtc acatcgcgca gaagcaggcc acgcccaatg gcccgccctg
      841 actcgtcacc cacgtcgaac agccgcaggt ggatggcacg gtgccggtca tagcgcctga
      901 tttcaacaac caactccgtg gtttcggtgg actcagcctg agctaaaatc cgctcgcaat
      961 ccgcgggatc gactgccctt gcacgcagcg catcgtagag ggtgctaatg gggtagccta
     1021 ccaacgcctc gctgggtaga cccacgatag tgcttgctcc ctggttggca tacagcacca
     1081 tcccgtcgat gcctgtcagc accagtccgt cgctcatact gctcatgatc gcctccagct
     1141 tctgtttttc ttccgaggca tgctggtaaa gccgctcgtt ctcttgtgcc accgcgcgga
     1201 gtctttcatc gctgcgctca taaagcactg catgttccca ggcaagtgta gcgtagttgg
     1261 cgaacgtcag cagcaagtta atctcgttct ggttgaaagg acggggctcg gtacgatgca
     1321 ccagcagaat cacacctccc acatgacgac tgatgatcgg aatcgccagc accgagcgga
     1381 atccttcctc gtaagagagc gaaggcattg gatgttcggg accgagcagt ttctgcaccg
     1441 gtttgccgtc gcgtagtgcc aacaccggtg ccgaactcac gttctcagga gacagggaga
     1501 gcacgttgtg gtagcgttca ctatggccgg cgcttgccag cacgcgcagc acaccatgct
     1561 cgtctgggag aagcactgcc gcggcctgca catccaccag ccgtcgcacc tcgccaatga
     1621 tctttcctac gacagcctga ggttcaagcg acccgaccac ggcgttcgac gtttcaagca
     1681 gcgtgcgcag ttcgccaagt tttttcagcc tgtcgagctc cagacggacg agcgaacgtg
     1741 ccagatcccc aacctcgtca ttgcgtgcag cgaggacgtt cgtatgtact gggatggatt
     1801 gctccgagac aggcagcgcc tgatgctgga tggcgagggt gtggagaggt cggatgaccc
     1861 ggaccagcag catgagccag aacagcaggc caccgatggc gaagagcaat gcggcagtca
     1921 gcaaccagag atggaactga gcgacgacgg cctgggcctc ggctgcaggt cgctgcacga
     1981 cgacggccca gccagcatcc gggaccggaa cggcactgaa aagccagtcc tggccaccgg
     2041 gccctggact caggcgtgag gcagtgtggc cttgcagcgc ctggtcggcg cccggcagtt
     2101 cgtccagcac cgtctgcaag atccgcgtgt tatcgggcgt gg
//
LOCUS       DASZAK010000951         4855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_485701, whole genome shotgun sequence.
ACCESSION   DASZAK010000951 DASZAK010000000
VERSION     DASZAK010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485701"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1668)
                     /locus_tag="VGT44_16660"
     CDS             complement(<1..1668)
                     /locus_tag="VGT44_16660"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2582491.1"
                     /translation="MQTTSIDQLAGSVLGDYQVERLLGNGHYGTAYLARQRSQGRQVV
                     ITTFNMREEMVAWQRDHLADRIAQERVLLTRLAHPNILPTYDFGAYANQLYLVTAVFN
                     GISLSQALKQQKRLTPQQTLDVLKQMASCLDYAHSSGVVHGLLSPSQVQIGARLQIAG
                     FGLRTILEVRQPQAQHFPLNATGAIQDNLEYSAPECVQGGPIDARSDIYALGVMLFEL
                     LSGSLPFSGQTPFESTLQRMHQSAHSVHSLCPDVPEALDLVIDKMLERDPVKRYKRAS
                     EGAVAFERVLQSFEAMERASTARSSRLVLESQQMLPPPVNEGKQAKPISRSFSLAGID
                     PFAWWTSMTTKDPQVAPGSFARGSQRQPVRLSDKRGRRRPIRQDRRQVVKMVATSATI
                     VGVFAIGGISFEHFVQSLKQPQQSATGSILPGSTTTTQGNTPAAGTTPSTQKSPSASK
                     TATPKASPTHSTQPSPTGQPTTQPTGQPTPQPTTPPTPQPTPQPTQPPTPTPSPTPSH
                     TGTVIGQTSQPANSAVKFTNPADGNAGLLIHLGNGNFVACERACTHAG"
     gene            complement(1834..2925)
                     /locus_tag="VGT44_16665"
     CDS             complement(1834..2925)
                     /locus_tag="VGT44_16665"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEV2582492.1"
                     /translation="MNSEEYLDRMIEQGQRGEIWLPEDNQEVATCLVAAETLTRLYEI
                     NVPPEFARRLEINVRARARGFSQQHENVAALALPQSMVGTLRSQPLRPQRRRAWLSGL
                     GVAAALALMFVGVLAVSASSLPGDLLYGIKQVENRLSLTFAGDPKDHAEIAISQLQNA
                     LVDLNSVVNEKRDDNTISMALDIVASNTSSSQQAVSALPAGPVREAAQHNLDSALAGE
                     QQTLKLLMNRLDWQMQLAFTHQLGVLGATVPTITSAQVRIQSNGQLLITLTGAHFSPK
                     AEFIFNGWQVGTISKITPTQLIAVINRSLWAGDSNALGLRNPDGTAAQTLYHVSDNDG
                     DEQNSNQNSATPTGSGGRGTDSSDGGFDE"
     gene            complement(2922..3701)
                     /locus_tag="VGT44_16670"
     CDS             complement(2922..3701)
                     /locus_tag="VGT44_16670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2582493.1"
                     /translation="MSAYPYTSLEINWGDERTRLSGIHQQGYFARFWGEISDSQCVAG
                     RCATNYSTSVSTTTFSQMIRQARLHDEEALVALYQRALPIVYRFVLARLGRYDLAEDV
                     VSEVFLAMVESISDLRTEQEAGFYAWLIQIAQGKVSRAIRRLAHKESRHVPLPDNRAS
                     DHAPDLELMATDLLSDPVALHEWRETLEEVGLALGHLSAEQQVVVIGRFLAGQKIEDL
                     AQALGKQPGAVRVLQFRALKTMAKHLGLNQDPQYRSKGGRA"
     gene            3884..4681
                     /locus_tag="VGT44_16675"
     CDS             3884..4681
                     /locus_tag="VGT44_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582494.1"
                     /translation="MKYYYTAQEARQQLGVNVGAFYYLVDTGKIKKVTPPGKKQGFYS
                     RHQIERLAQEEATGLVADKHAGLIFKQATLDDLAEEYELALLTLNGSAGYGLPAYETW
                     LRKNAATNFLVRDHGRLVAFMHVLPVTQGCIQRWIKGEIREWEIEAEDVQPYASQRTV
                     ECLITCMVTTPDVGERERRLYGMRLIHGYLQFIHHLARLGITISRYYAAGSSTEVIDM
                     LKRAKFEHSGQVGKRTAFELNPLTAKTHMAKHYRAILKRHNLRVEAR"
BASE COUNT         1066 a         1308 c         1391 g         1090 t
ORIGIN      
        1 gcccgcatgg gtgcaggctc tttcgcaggc gacaaaattg ccattgccaa gatggataag
       61 caggccggcg ttgccatcag ccgggttagt aaatttcacc gcactatttg ccggctggct
      121 ggtttgaccg atcacggttc ccgtatgcga cggggtcggc gacggggtcg gcgtcggcgg
      181 ctgggtcggt tgcggagtgg gttgtggtgt tggtggcgtg gtcggctgtg gtgtcggttg
      241 accggtcggc tgcgtggtcg gttgaccggt cggagatggt tgcgttgaat gggtaggcga
      301 tgctttcggc gttgccgtct tcgatgccga aggtgatttc tgagtagacg gggtagttcc
      361 cgctgctggc gtattcccct gggtagtcgt ggttgagccg ggcaggatgg aacctgtggc
      421 agactgttgt ggctgcttta agctctgtac gaaatgctca aaactgatcc cgccgatggc
      481 aaacaccccc acgatagtcg cgctggtagc gaccattttt acaacttgcc gccggtcctg
      541 gcgaattggc cggcgacgcc cgcgcttatc tgacagacgc acaggctgtc gctgcgagcc
      601 acgggcgaac gagcctggcg cgacctgcgg gtctttcgtc gtcatgctgg tccaccaggc
      661 gaacgggtcg atgccggcca gtgagaaaga acgactgatg ggcttagcct gtttaccctc
      721 gttgactggg ggtggaagca tttgctgcga ctccagcacc aaccgcgaag accgggcggt
      781 cgaggctctc tccatagcct caaacgactg aagcacgcgc tcaaatgcga ctgcgccctc
      841 gctggcgcgt ttgtatcgct tcaccgggtc gcgttccagc attttgtcga tgaccaggtc
      901 aagcgcttcc ggcacgtctg gacagagcga gtgaacagaa tgagccgact ggtgcattcg
      961 ctgtagagtg ctctcaaagg gagtctgccc gctgaaaggc agagagccac tcaacagttc
     1021 aaagagcatg acacccagcg cgtaaatatc tgatcgggca tcgataggcc caccctggac
     1081 gcattcaggc gcactatatt cgagattgtc ctggatcgcg cctgtggcgt ttaaaggaaa
     1141 atgctgggct tgaggctgtc gtacttccag aattgtcctc aggccaaagc cggcaatttg
     1201 caaccgcgcg ccaatttgca cctgtgaagg actcaacaac ccgtgcacga cgccgctgct
     1261 gtgcgcatag tccaggcacg aagccatttg tttgagcaca tcgagcgttt gttggggcgt
     1321 caggcgcttc tgctgtttga gggcctggct gagcgaaata ccgttgaaaa cggctgtcac
     1381 caggtacagt tgattggcat acgcgccgaa atcatatgtt ggcaagatat tcggatgcgc
     1441 caggcgagtc agcagcactc gttcctgggc aatgcgatct gccaaatggt ctcgttgcca
     1501 ggctaccatt tcttcccgca tgttgaaggt cgttatcacg acctggcgcc cctgcgaacg
     1561 ttgtcgagcc aggtatgccg taccgtagtg gccattccca agcaagcgtt ctacctggta
     1621 atcccccagt acactgcctg cgagttggtc gatactcgtt gtttgcacta tccaatttcc
     1681 tttctgcata caaatcacgt gtgacaggtg gcagtgtacc tgtagatttc atcttgcgtc
     1741 aatgcggtga gtgtacgaat aggaatgctg ggataaacgc agtaaccggc atctcaacca
     1801 taattagctt cgatgcaaca gagaatctct tcatcactca tcgaaaccgc catcggatga
     1861 atctgtaccg cgaccgccgc tgccagttgg agtagcgctg ttttgattgc tgttctgttc
     1921 atccccatcg ttatcactga catgataaag cgtctgagca gcagttccgt cggggttgcg
     1981 tagaccgagt gcgtttgagt cgccagccca cagcgaacga ttgatgactg cgatcaactg
     2041 agtaggagtt attttgctta tcgttcccac ctgccatccg ttgaagatga actctgcctt
     2101 cggcgaaaag tgcgcgcccg tcagggtaat caacagttgg ccattgctct ggatacgcac
     2161 ctgggcgctc gttatggttg gcacggtcgc gccgaggact cccaactggt gggtgaaagc
     2221 aagctgcatc tgccaatcca ggcgattcat caggagtttt agggtctgtt gttccccggc
     2281 aagcgcactg tcgagattgt gctgcgcagc ctcgcgcacg gggccagcag gaagagctga
     2341 gacggcttgc tggctgctgc tcgtattgga agccacgatg tccagcgcca tgctgattgt
     2401 gttatcgtca cgtttttcat tgacgacgct attcagatca acgagcgcat tctgtagctg
     2461 gcttatcgct atttcagcgt ggtccttcgg gtcgccagca aacgtcagtg atagccggtt
     2521 ttcgacctgc ttgatgccat agagcagatc gcccggcagg ctgctagcag agaccgccag
     2581 cacaccgaca aacatcagtg ccagggcggc agcgacaccc agcccggaga gccaggcgcg
     2641 ccttctttgg ggacgcagag gttgcgagcg caaagttcca accatacttt gagggagcgc
     2701 cagcgcagcg acattttcat gctgctgtga aaacccgcgg gcgcgggcgc gaacattgat
     2761 ttccagacgc cgcgcaaatt caggcgggac attgatttca tagagcctgg tcagcgtttc
     2821 ggctgcaacc aggcaggtag cgacctcctg attgtcttcg ggaagccaga tttctccgcg
     2881 ttggccttgc tcgatcatgc gatccaggta ttcttcggaa ttcatgctcg ccctcctttg
     2941 ctcctgtatt gggggtcctg attcagtccc aaatgctttg ccatcgtctt taaggcgcgg
     3001 aattgtaaca ctcgtactgc ccccggttgc ttccccagag cctgggcgag gtcttcgatt
     3061 ttttgccctg cgagaaagcg gccaatcacg actacctgct gttcggcgct cagatgcccc
     3121 aacgccagac ccacttcctc aagcgtttcg cgccattcgt gcaatgctac cgggtcgctc
     3181 aacaaatcgg tagccatcag ctccaggtca ggcgcgtggt cacttgcgcg attatccggc
     3241 agggggacgt gcctgctctc cttgtgtgcc agtctcctaa tggctctgga gactttgccc
     3301 tgggcaattt gaatcagcca ggcataaaaa ccggcctctt gctcggtacg aagatcagaa
     3361 atggactcta ccattgccag gaacacttcg gatacgacat cttccgcaag gtcataacgg
     3421 cccaggcgcg ccagcacaaa acgatacaca atcggaaggg cgcgctggta gagcgcgacg
     3481 agcgcttctt cgtcatgaag gcgcgcctgc ctgatcattt gtgaaaaggt agtagtagag
     3541 acggaagtac tgtagttcgt tgcgcacctc ccagcgacac actgcgagtc gctgatttcc
     3601 ccccagaaac gagcaaagta accctgttgg tgaattcccg aaaggcgcgt cctttcgtca
     3661 ccccagttga tttcgaggga agtgtatggg taagcgctca actttgtcaa cgcgccgtgc
     3721 ttgcagtagg atttgtggac gatttgccgc gaaagaactt aaccagaatt tgaatatcaa
     3781 cttgaaaaga cagggtgtaa caaattgata gtaattggca ctacttctcc gaattgcaat
     3841 agcagacctg gcatccgcca ggaaatgcca tgggaggtac agcatgaaat actattacac
     3901 cgcgcaggag gcgcgacaac aattgggggt caacgtggga gctttctatt atctcgtcga
     3961 tacagggaag attaagaagg tgacgccccc tggaaaaaaa cagggatttt actcaaggca
     4021 tcaaatcgag cgactggcgc aagaggaagc gaccggcctg gtcgcagaca agcacgcggg
     4081 tctgatattt aaacaggcaa ccctggatga cctcgctgag gagtacgaat tagcattgct
     4141 gacgctcaat ggcagcgccg gatatggcct gccagcgtat gagacctggc tgcggaaaaa
     4201 tgccgcgacc aattttcttg tgcgagatca tggtcggtta gtagcattca tgcacgtgct
     4261 tccagtcacg caggggtgca tccaacgctg gatcaaggga gagatcaggg aatgggaaat
     4321 cgaagcagaa gatgttcaac cctacgcctc gcaacgtacg gtggaatgcc tgatcacttg
     4381 catggtgaca acacctgatg taggcgaacg ggaaaggcgc ctgtacggga tgcgcctcat
     4441 tcacggctac ctgcaattta tacatcacct ggcaaggctg ggcatcacga tcagcaggta
     4501 ttatgccgca ggcagctcca ccgaagtgat cgatatgctc aaacgagcaa agtttgagca
     4561 tagtggacag gtagggaagc gcaccgcgtt tgaactcaac cctctcaccg ccaaaacgca
     4621 catggcaaag cactatagag caattttgaa gcgtcacaac ctgcgcgtcg aagcgcgcta
     4681 aaagactgtc acaccgcagc cgggggtgta agcaaacccg taggtgccga tttatcgcgc
     4741 acaccgccga ttgatcggcc tggttgcttg ccgttaacgc ccatgatggc cgataatgat
     4801 ttttcacgac caacattgga catctcgtag gagccgattt atcgtgctcg cagcc
//
LOCUS       DASZAK010000952         4855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_24555, whole genome shotgun sequence.
ACCESSION   DASZAK010000952 DASZAK010000000
VERSION     DASZAK010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24555"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VGT44_16680"
     CDS             complement(<1..697)
                     /locus_tag="VGT44_16680"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2582495.1"
                     /translation="MRRTIFTEEHQMFRDSVRHFVENEIVPFYDQWEKDGIVSREVWL
                     KAGQQGFLGFNVSEQYGGEGVDDYCYSVIFAEETSRVGAVSVGSGMGLHNDIVIPYFL
                     AYANEEQKRRWLPGMCRGELITAIAMTEPNTGSDLAAVRTSAERRGDEYILNGQKTFI
                     TNGILSDVVVVVAKTDPTLGHKGISLLVVERGMEGFERGRKLDKVGMKAQDTAELVFE
                     DVHVPLSNLLGEEG"
     gene            complement(694..2049)
                     /locus_tag="VGT44_16685"
     CDS             complement(694..2049)
                     /locus_tag="VGT44_16685"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEV2582496.1"
                     /translation="METKLSWHEHLKRERIKRGWSQGDVANRIGSDNKTVSRWERGIT
                     FPDPYNRQHLAKLYKKSVEELGLVEQDAGETADEVQSVLRRARLEDWGEAPDVERFYG
                     RAEELCELEHWIIDEHCRLVTLLGMGGVGKTALAIRAAKQVQVTHPFEFIFWRSLQNA
                     PAVESLLGNCLQNLCRERVVDVSRNLDEQISLLIACLKEHRCLFILDNVESVLQAERR
                     VGQYLESYEGYGLLFQRIGEISHQSCLMLTSREKPGEVARLEGVESPVRSLRLDGMEP
                     GSGQELLKDKGLFGTDNLWRTLVRRYAGNPLALKLAAEPIREVFGGDIAAFLEGDEVV
                     FGDIFDLLEQQFQRLSTMERYVLYWLAIERENVSLHTLEKKLRHQINRRTLLEALDSL
                     HRRSIIEVRSDGQFLLQPLIMDYVTGKYVALLPVFIGGCIIPGKDALSCARPPLALEA
                     T"
     gene            complement(2273..3238)
                     /locus_tag="VGT44_16690"
     CDS             complement(2273..3238)
                     /locus_tag="VGT44_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2582497.1"
                     /translation="MQIQETQFPTGTSVPVIRPGDAGQTPFARVLRVLLSLWRVITTS
                     RRMSVGVAIVGIFILVGLFGPLIDRTNPNAISNLFIASPSAAHWLGTSVVGEDLFSQL
                     INGTRTSVFWGLGTGLVVTAISALVGLVGGYFGGWIDDVLTLLTNVSLVLPALPLAVV
                     LAAYFPRGPLTISLVIIATNWAWQARVLRSQTLSMRNREFITAARTSGEHTWRIIFFE
                     IFPNELGLVVAGFVSTTIYVVLTWAALEFLGLGDGTIPSWGSMLYWAQQAGALGGGLW
                     WWFVPPGACIAILGAGLSLINFGIDEIADPRLRVERPAKSVKNPR"
     gene            complement(3241..4242)
                     /locus_tag="VGT44_16695"
     CDS             complement(3241..4242)
                     /locus_tag="VGT44_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2582498.1"
                     /translation="MRHVIRRIIFYACAVWVAITLDFFIPRLAPGDPVAALVGKMSTK
                     GYVTPEMQQNLAAMFGLNTHDPLWLQYFKYLGNLLHGNLGNSIEYFPTPVAHIILQDI
                     GWSLMLGGVAVFISFMLGCLLGIITAWKRGSMLDTILSPAMNFLSAIPYFWLALLSLY
                     IFAYLLSWFPLTGGGYDAANIDPGWTFDYIGSIIQYAFLPALTLVISSLAGWMLTMRN
                     SMITTLSEDYVLMARAKGLSTMRVMFMYAARNAVLPNITGFAIAIGAIVGGQLLTEMV
                     FSYPGIGYALLQAVSEQDYAMVQSIFLIITLAVRGANFIADMLYVFLDPRVRSERGA"
     gene            complement(4454..>4855)
                     /locus_tag="VGT44_16700"
     CDS             complement(4454..>4855)
                     /locus_tag="VGT44_16700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2582499.1"
                     /translation="YTLRTDGKYDYQMMFCGMVGGPTPYYLYNQYLNSNQIGQGKFNF
                     VAWNDPKTDQFLNTYASTTDPTVQKQAIQGIEQVFVQNQPFIPLWTGADYDEYSTLHF
                     TGWPSQADPYSSGSPNTAPDYEQVILHLQPV"
BASE COUNT         1143 a         1464 c         1255 g          993 t
ORIGIN      
        1 tgccctcctc accgagcagg ttgctgagcg gtacatgcac atcctcgaag accagttccg
       61 ccgtatcctg cgccttcatg cccaccttgt ctagcttgcg cccacgctcg aagccctcca
      121 tgccgcgttc cacgacgagc aggctgatgc ccttgtgtcc tagcgtcgga tcggtcttgg
      181 ccacgacaac gaccacgtcg ctcaggatgc cattggtgat gaaggtcttc tgcccgttga
      241 ggatatactc atcgcccctg cgttcggcgc tcgtgcgcac cgccgccagg tcgctgcccg
      301 tattcggctc ggtcatggcg atggccgtta tcaactcccc ccggcacatg cccggcagcc
      361 agcgccgctt ctgctcctcg ttcgcatacg ccagaaaata gggaatgaca atatcgttgt
      421 gcagccccat gcccgaccca acgctgaccg ctcctacccg cgaggtctcc tccgcgaaaa
      481 tcacgctgta gcaataatca tctacccctt cgcctccata ttgctccgac acgttgaatc
      541 ccaggaagcc ctgctgaccg gccttcaacc atacttcccg cgagacgatg ccatctttct
      601 cccattgatc gtaaaacggc acaatctcat tttccacaaa atgacgaacg gaatcacgaa
      661 acatctggtg ttcttcggta aatatggtcc ttctcatgta gcctccaaag caagtggcgg
      721 tctggcacaa ctcaacgcat ctttgccagg tattatacat cccccaatga agactggcaa
      781 aagcgcaacg tacttccccg tgacgtaatc cataatgagc ggttgaagga ggaattggcc
      841 gtcactgcgt acttcgatga tggagcgcct gtggagtgaa tctaacgcct cgagcaacgt
      901 tcgtcgatta atctggtgtc gtaatttctt ctccaacgta tgaagcgaga cattctcgcg
      961 ctcgatggcc agccaataca atacatatcg ttccattgtt gataagcgct gaaactgctg
     1021 ttcgaggaga tcaaaaatat cgccgaacac gacctcatcc ccttccagaa aagccgctat
     1081 atctccgcca aacacctctc gaataggctc agcggccagc tttagcgcaa gaggattgcc
     1141 cgcataacga cgaacaaggg tcctccacag attatcagtt ccaaaaagac ccttgtcttt
     1201 caaaagctct tgcccacttc ctggctccat gccgtccagc cgcagcgaac gaaccggaga
     1261 ttcaacgccc tccagccgcg caacttcccc cggcttttca cggctggtca acatcaggca
     1321 actttgatgg cttatctctc caatgcgctg gaagagcagt ccatatcctt cataggactc
     1381 caggtactgg cccacacgtc gctcagcctg taacaccgac tcgacattgt ccaggataaa
     1441 caggcagcga tgctctttca aacaggcgat aagcaaggag atctgctcat caaggttcct
     1501 tgacacgtct accactcgtt cccggcagag attttggaga cagtttccca gcagactttc
     1561 cactgctgga gcgttctgga gcgaacgcca gaagatgaat tcaaatggat gtgttacctg
     1621 cacctgcttt gctgccctaa ttgccagagc cgtcttgcca acgccgccca tacccaggag
     1681 tgttactaga cggcagtgtt catctataat ccagtgttcc agctcgcaca gttcctctgc
     1741 tcgtccataa aaacgttcaa catccggcgc ttccccccaa tcttccagcc gggccctgcg
     1801 aagcaccgac tgaacttcat cagcagtttc ccctgcatcc tgctctacta accccagctc
     1861 ttcgacgctt tttttataga gttttgcgag atgctggcga ttataggggt ctgggaaggt
     1921 tattccccgt tcccaacggc tgaccgtctt gttatcgctt ccaattctgt tggctacatc
     1981 accttgcgac caaccgcgct ttatgcgttc acgtttcaaa tgctcgtgcc aggacagttt
     2041 cgtttccact agaatccttt ctaactctgt acatcaagac gctttatcac catgaacaca
     2101 tacggcaaga atatatcctt acttgttcat taaataaatg ctggaaatgc atccaaatag
     2161 gatgaaaaac ttcgctctca ccaataataa gacggaaaac ttgagctacc atcaaaaaat
     2221 gttttgagaa agctcaagtt ttccgctcta taaatcagcg aggctgagaa aattacctgg
     2281 ggttcttcac cgacttcgcc gggcgttcca cccgcaggcg ggggtcggcg atttcgtcga
     2341 tgccgaagtt gatcagcgag aggcccgcgc ccagaatagc gatacacgcg ccgggcggca
     2401 cgaaccacca ccacaggccg ccgcccagcg cgcccgcctg ctgcgcccag tacagcatgc
     2461 tgccccagct cggaatggtg ccgtccccca ggccgagaaa ttcgagcgcc gcccaggtca
     2521 acacgacgta gatggtcgtg ctgacgaaac ccgccaccac cagcccgagt tcgttgggga
     2581 atatctcgaa gaagataatg cgccaggtat gctcgccact ggtgcgcgcc gccgtaatga
     2641 attcacggtt gcgcatcgag agcgtctgcg agcgcaaaac acgcgcctgc caggcccagt
     2701 tggtggcgat aatcaccagg gagatcgtca acgggccgcg cgggaaatag gcggcgagga
     2761 cgacggcgag cgggagcgcg ggcagcacca gcgaaacgtt ggtaagcagc gtcagcacat
     2821 catcgatcca cccgccaaaa tagccgccga ccagcccgac cagcgcggag atggccgtta
     2881 ccaccaggcc agtccccagg ccccagaaga cactggtgcg tgtgccattg atcaactggc
     2941 tgaagagatc ctcaccaacg accgatgtgc cgagccagtg cgccgcggag ggggatgcga
     3001 tgaacaggtt cgatatagcg tttggattag ttctgtcgat gagcggccca aagaggccaa
     3061 ccaggatgaa aatgccgacg atggccacgc caaccgacat tctgcggctc gtggttatga
     3121 cccgccagag ggagagcagc actcgcaaga cacgcgcaaa cggggtctga ccggcatcac
     3181 ctggtcgtat gaccggcacc gacgtaccgg taggaaattg cgtctcctgg atttgcattg
     3241 ctacgcgccc ctctctgaac gcacacgcgg atcgagaaaa acgtacaaca tatctgcaat
     3301 gaaattcgcc ccccgcacgg ccagcgtgat aattagaaaa atgctctgca ccatcgcgta
     3361 atcttgctcg ctcacggcct gcagcagcgc atagccgatg ccgggatagg agaagaccat
     3421 ctcggtcagc agctggccac cgacaatcgc gccgatggcg atggcgaagc cggtgatatt
     3481 cggcagcacc gcgttgcgag cagcgtacat gaacatcacc cgcatggtgg acagcccttt
     3541 ggcccgcgcc atcaacacat aatcttccga gagcgtcgta atcatggagt tacgcatcgt
     3601 cagcatccag cccgccagcg acgagatcac cagcgttaag gccggcaaaa aggcgtactg
     3661 gatgatgctg ccgatatagt cgaacgtcca gcccggatcg atattggccg cgtcataccc
     3721 gccgccagtc aagggaaacc agctcaacag gtaggcgaag atatagagcg aaagcagcgc
     3781 cagccagaag tagggaatgg ccgacaggaa gttcatcgcg ggcgaaagga tcgtatccag
     3841 catcgagccg cgcttccagg cggtaatgat gccaagcaga cagcccagca tgaaactgat
     3901 gaaaacggcc acgccgccca acataagcga ccagccgata tcctgcagga tgatatgcgc
     3961 gacaggcgtt ggaaaatact cgatggaatt gcccagattg ccatgcagca ggttgccgag
     4021 gtatttgaaa tattgcagcc agagtgggtc atgggtattc aagccaaaca tagccgccag
     4081 gttctgctgc atctccggcg tgacatatcc ctttgtggac atcttcccca ccagcgctgc
     4141 caccgggtcc ccaggagcca ggcgcggaat gaagaaatcg agcgtgatcg ctacccagac
     4201 ggcgcaggca taaaaaataa tgcgacgaat gacatgacgc acctggctgc tccttctact
     4261 gacatttgta gcgaaaccga cgtatgacct ggcatttctc ttgaatcgct tgccaacgag
     4321 ttacggcggg cgacgcaagc gtcccctccc atcccggcac cacccccgcc cctacggtac
     4381 atacgtcgga tgcggcgaaa cgttcacgtt ggcgttcccc cggcgcagtg ccggggaacg
     4441 ccttcgtgta ttactaaacg ggctgcaggt gcaggatgac ctgctcgtag tccggggcag
     4501 tgttggggct gccgctggaa tatggatcag cctgactggg ccagccggtg aagtgcaggg
     4561 tggaatactc gtcgtagtca gcgcccgtcc atagggggat gaagggctgg ttctggacga
     4621 agacctgctc gataccctga atcgcctgct tctgcactgt cggatcggtc gtggaagcat
     4681 acgtgttcag aaactggtcg gtcttggggt cattccaggc cacgaaattg aacttgccct
     4741 ggccaatctg attgctgttg aggtactggt tgtacaggta gtatggcgta gggccgccta
     4801 ccatgccgca gaacatcatc tggtaatcat acttgccgtc ggtgcgcagg gtata
//
LOCUS       DASZAK010000953         1523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_664356, whole genome shotgun sequence.
ACCESSION   DASZAK010000953 DASZAK010000000
VERSION     DASZAK010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664356"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VGT44_16705"
     CDS             complement(<1..552)
                     /locus_tag="VGT44_16705"
                     /inference="COORDINATES: protein motif:HMM:NF042769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEV2582500.1"
                     /translation="MTDQIQLEPEAHAFAEAAARSPLLFTLGPEQGRLALDQVQSGKV
                     PKPAVDMEDLTITDGPSAQVALRILRPQNAQAPLPVIVYIHGAGWVFGSPQTHDRLVR
                     ELAVGAGAAVVFPVYRLCPEVRYPTALEECYTAARWVGRFGREHGLDAERLAVVGDCV
                     GGNLATVLTLLSRERGEPDIRLQV"
     gene            complement(615..1037)
                     /locus_tag="VGT44_16710"
     CDS             complement(615..1037)
                     /locus_tag="VGT44_16710"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV2582501.1"
                     /translation="MDQPAPGHDWIALVRKHGTDEFGRAFAPNATLDASVLNGPCVGV
                     QAIAAFFAATSGGMYETLEFTHETTDGRKTYLEWQGTAFGKQVRGTTILTRNHEGLIE
                     HIQLYHSPLQVVVEFAQELEKRLEAESRSVCTELNEER"
     gene            complement(1124..>1523)
                     /locus_tag="VGT44_16715"
     CDS             complement(1124..>1523)
                     /locus_tag="VGT44_16715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582502.1"
                     /translation="ADPKQLAIDAATTVKTIEGVPDIPMFRKNTAAFVHELPAGTLAN
                     VDGDETYVRVQVLTNEGALDREKQLAVVAQLTQLVSTAAGDETLKDRTWVLLTEAKEG
                     GWGLWGHAHTNAELVAAARGEIAKLRSTSS"
BASE COUNT          277 a          474 c          432 g          340 t
ORIGIN      
        1 cacctgcagc cggatatccg gttccccgcg ctcccgggac aagagcgtga gcaccgtggc
       61 cagatttccc cccacgcaat cgccgaccac cgcgaggcgc tcggcatcca ggccatgttc
      121 ccgtccgaat ctcccgaccc agcgtgccgc cgtgtagcac tcctcgaggg cggtcgggta
      181 tctcacctcc ggacagagcc ggtacacggg gaagacgacg gcggccccag ctccgaccgc
      241 cagttcgcgc accagccggt catgcgtctg cggactgccg aagacccacc cagcgccatg
      301 aatatacacg atgactggca gcggagcctg cgcgttttgg ggccgcagaa tacgcaacgc
      361 cacctgcgcg ctgggaccat cggtaatagt caggtcttcc atgtcgaccg cgggcttggg
      421 aaccttgcca gattgcactt gatcgagagc gagccgtccc tgctctgggc caagcgtgaa
      481 cagcaaaggt gatcgagcag ccgcctctgc gaaggcgtgc gcctcgggtt caagctgaat
      541 ctggtcggtc atggatctcg ctcctcaatg acatctgaca gcgcacagcg tgtctgctgc
      601 acaagaacgt tcctttatcg ctcctcattc agttcagtac agactgatct actttccgct
      661 tccagacgct tttccagttc ttgagcaaac tccacgacca cctgcaaggg gctatggtag
      721 agctggatat gttcaatcag cccctcatgg ttgcgtgtca gaatcgtggt tccgcggacc
      781 tgtttcccaa aggctgttcc ttgccattcg agataggttt tcctgccatc ggtggtttcg
      841 tgcgtgaatt ctagggtttc atacatgccg ccggacgtgg ccgcgaagaa agccgcgatg
      901 gcttggacgc cgacacaggg gccgttcagg accgaagcgt caagcgtcgc attcggagca
      961 aatgccctgc cgaattcgtc ggtgccatgt tttctgacga gtgcaatcca gtcgtgtcct
     1021 ggtgcgggct gatccatgat ggcataccct tcggataagc gtgtgagcaa gcggtctgtc
     1081 ctaacaaggt gctctggtgt tctttcgctc tcccatgacg ctgttaactt gatgtagacc
     1141 tcagcttcgc gatttcaccg cgtgctgcgg ccactaattc cgcattcgtg tgagcatggc
     1201 cccagaggcc ccagccgcct tctttcgctt ctgttaagag cacccacgtg cggtctttca
     1261 gtgtttcgtc accggcagca gtgctcacga gctgtgtcag ctgtgctacc acggcgagct
     1321 gtttttcgcg gtccagggca ccctcgttgg tcaggacctg cacgcgtaca taggtttcgt
     1381 caccatccac attggcgagt gtgcctgctg gcaactcgtg cacgaaggcg gcggtgtttt
     1441 tgcggaacat cgggatatcc ggcactccct cgatcgtttt caccgtagtg gccgcatcaa
     1501 tggccagttg cttcggatcg gca
//
LOCUS       DASZAK010000954         1935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_522861, whole genome shotgun sequence.
ACCESSION   DASZAK010000954 DASZAK010000000
VERSION     DASZAK010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522861"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..434)
                     /locus_tag="VGT44_16720"
     CDS             complement(51..434)
                     /locus_tag="VGT44_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582503.1"
                     /translation="MSTVTPESKPIKTNIAPWLLVSRATEAVDFYKAAFGAVELYRLE
                     DDAGKVVVAQLAIEGADFWLQEDVDSSPEAQGCQSVRMILTVADPDSVFEQSRRSKGI
                     QCCFRGSRLAHRTHRRPVWSSLGDW"
     gene            complement(693..>1935)
                     /locus_tag="VGT44_16725"
     CDS             complement(693..>1935)
                     /locus_tag="VGT44_16725"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582504.1"
                     /translation="DATRWSWVFYINLPVGILALAALLIYLPTNISQRQTAYAGWAAL
                     RRIDFLGALLVASATICLLLGLTWGSNATFAWASAQVIGVLVGCGVLYVLFFVVERFA
                     REPVLPLDLFRNQVFSMALLLSFLQLMVLVGLIVYLPLFLQGVLGVSPTNSGAAITPL
                     TVSSVIGAALAGILVARFQRYQLVTIVSSIIMAIGLFLLTRISATINLFEMIVFMVIA
                     GLGLGPFFSVLQLAAQNSIPRARLGVGTAAVRFVGQIGAVLGVALVGTVVNQTLADDI
                     VKRIPASTVQQLTPAGFKAATDPQILVNSTYRDTVVHTAQRFAVQHAVAQIPPGPQHD
                     QVAASVATQVQSMLNQVFDALKISLTFAIQHGLVAVFVFSLGMVLAAFFLKDIPLSKE
                     HSPAPARVEQPETSEENLPIV"
BASE COUNT          477 a          554 c          570 g          334 t
ORIGIN      
        1 aagcgaaacc gaaaatgaat gattaagggt acgcaaatca gcttaacggc ctaccaatct
       61 cccaatgatg accaaacggg tcggcgatgc gtccgatgcg ccaaccgtga tcctcggaaa
      121 cagcactgaa ttcccttgct cctgcgggac tgctcgaaaa ccgagtccgg gtcggccacg
      181 gtcaatatca tcctgaccga ttggcagccc tgagcctcag ggctggagtc gacatcctcc
      241 tgaagccaga aatctgctcc ctcgatagcc aactgagcga cgacgacctt tccggcgtca
      301 tcttcaaggc ggtacaattc gaccgcgcca aacgcggcct tataaaagtc caccgcttcg
      361 gtagcccggc tgaccaacaa ccacggggcg atgttagtct tgattggttt ggactcgggt
      421 gtaactgtac tcattatcat agttctcctt acagacacgt actgtcgccg gactctcagc
      481 gaggggacgc gcgcttttat aacagaatgg cgctttcgcg atagttaggt agtcgctcga
      541 taaggttttc gccagcataa cccgatgtaa cgcgaaaatg atcttcaccc tacgagtgta
      601 ccatgtaggc cacgcataga gacgagagcg ccaggcaaca aatgctgttg cctggcgctc
      661 tcgtctttga gtaaagctaa agcacaacgt tactacacga taggcagatt ctcctcgctg
      721 gtctctggct gctcaacccg agccggtgca ggcgaatgtt ccttcgacag gggaatatcc
      781 ttcaggaaga aggcggccag caccatcccc agactgaaga cgaagaccgc cacgaggcca
      841 tgttgaatgg cgaaggtcag cgagatcttg agggcgtcga agacctggtt gagcatcgac
      901 tgcacctgtg tggcaacgga ggctgcaacc tggtcgtgct gcgggccggg cgggatttgc
      961 gcgacggcgt gctgcaccgc gaaccgttga gcggtatgca ccaccgtgtc acgataagtt
     1021 gaattgacca gtatctgtgg atcggtcgcg gctttgaaac cggcgggcgt aagttgctgc
     1081 acggttgagg cagggatgcg cttgacgata tcatcggcaa gcgtctggtt aaccaccgta
     1141 ccaacgaggg cgacgcccag caccgcgccg atctggccga cgaagcgcac cgccgccgtt
     1201 cctactccca ggcgagcgcg gggaatggag ttctgcgccg ccagctgcag aacggagaag
     1261 aacggcccca ggccgagtcc ggcgataacc atgaagacaa tcatctcaaa caggttgatc
     1321 gtagccgaaa tgcgcgtcag caggaatagg ccaatggcca tgatgatcga gctgacaatc
     1381 gtgacaagct ggtaacgctg gaagcgggca accagtatgc cagccagcgc cgcgccgatt
     1441 accgaactga ctgtcagtgg agtgatcgca gcccccgaat tagtcggtga aacacccagc
     1501 acgccctgca agaagagcgg caggtagacg atcaggccga ccagcaccat caactgcaaa
     1561 aacgagagca gcagggccat cgaaaagacc tggttgcgga acagatcgag cggcagaacg
     1621 ggttcgcggg cgaaacgctc tacgacgaag aagagtacgt agaggacgcc gcaacccacc
     1681 agtacgccga taacttgcgc ggaagcccag gcaaaggtag cattgctacc ccaggtcaag
     1741 cccagcagca ggcaaatcgt cgcggaggcg accagcagcg ctcccaggaa gtcgatgcgg
     1801 cgcaaggcag cccagccggc ataggcagtc tggcgttgtg agatgttcgt cggcaggtag
     1861 atcagcagag cagccagcgc gagaatgccc accggcaggt tgatgtagaa cacccagctc
     1921 cagcgcgtag cgtcg
//
LOCUS       DASZAK010000955         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_129894, whole genome shotgun sequence.
ACCESSION   DASZAK010000955 DASZAK010000000
VERSION     DASZAK010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129894"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1505
                     /gene="hutH"
                     /locus_tag="VGT44_16730"
     CDS             <1..1505
                     /gene="hutH"
                     /locus_tag="VGT44_16730"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEV2582505.1"
                     /translation="VLDGQGLTRPQVVAVARHFAPVTLGERSYERIQAARAVIETIAD
                     EGRKVYGVTTGFGHLSRVRIPHEQLTELQHNLLRSHASGVGEPLTEEVTRAMLLLLAA
                     SLARGHSGVRVEVVQLLTSMLNARIYPIIPSRGSVGASGDLAPLAHLALTLIGEGEAS
                     FEGKRMSGLDALRLAELNPIDLHAKEGLALINGTHMMEAIAILATHDAQVLIRAAEVA
                     CAMSIEGLMGSHAPLDARIHKRRGQHGQQISAARLRQLVEKSEINVSHADCPRVQDPY
                     SMRCAPQVLGAVRDALDVCSGIFERELDAVTDNPLIFPDDGDVLSGGNFHGQPLALAL
                     DVLAIALAQLASFSERRIYNLMGPHDWDEGGAPLFLTPNPGLNSGLMIVQYVAASLVN
                     EIKILAHPASIDSIPTSAGMEDFNSMGATSAHKLLRVLEQARQVVAIELMCAAQMLEF
                     RKPLKPGLGVLAAYDIVRSHVTRLEEDRVLTPDIDSLTKAIYEGAFESLA"
     gene            1816..3288
                     /locus_tag="VGT44_16735"
     CDS             1816..3288
                     /locus_tag="VGT44_16735"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV2582506.1"
                     /translation="MMITHDLPPGMHGSFPDPSLSHLPSDQIEQVRALRSEVVARYRP
                     HILEGPGPIRIMARLVAELEQLCREHAISDEVIQASIVNRTIGDVDLRRVTECEGEAG
                     GPGDFRLLADELGIALPGEQLPGYTASGETYLWLREQMQVLERTLLKQRIDVRMYDIF
                     GIGNPLLRGWLASMMQQQWGLPFTKEQIHLGIGAMDCVDKSLRSLRFVLMEQTGTNPA
                     ILFPEPGFNVPEWQARSYGYRMQHYKTDASRAFKLPAAQLEEILQNTPDIGVIYLTVT
                     NNPTTFAYNAQELDELFAAIRPYWEQGRHIYILADLAYVGTGDPADDAARMATFATPD
                     VMRHTIFVNSFSKTLSLTGDRFGYVAIGDPKLAPALGPGWTNSTAALPGEWQLRYMAV
                     VRLLQERPWLIAKLRALYRLRRRRFENQLHHINMEQPLFQHIYEGDDATVYTWCQLAH
                     GEDAFSLFEKTGIAGVPGSGFGYTDDFIRFSIGVIPVPEW"
BASE COUNT          682 a         1016 c          934 g          676 t
ORIGIN      
        1 tcgtgcttga tgggcagggt ttgacgcgcc cgcaggtggt agccgtcgcg cgacattttg
       61 cgccggtcac gctgggagag cgttcatatg agcgtatcca ggcggctcgc gcggtcatcg
      121 aaactatcgc ggacgaaggg cgcaaggttt acggtgtcac aaccggcttc ggacacctca
      181 gccgcgtgcg tatcccacat gaacaactga ccgagttaca gcataatctg ctgcgcagcc
      241 atgcatcagg tgtgggagaa cccctgactg aggaagtcac gcgcgccatg ctgctgctgc
      301 tggcggcgag cctggcgcgt ggtcattcag gcgtgagggt tgaggtggta cagcttctga
      361 caagtatgct taacgcgcgt atctatccca tcattccttc acgcggctcg gttggagcca
      421 gcggcgacct tgctccactg gctcatctcg ccttgacgct catcggcgag ggcgaggcca
      481 gcttcgaggg taaacgtatg agcggcctgg atgcgcttcg attggcagaa ttgaacccca
      541 tcgacttaca tgccaaagag ggcctggcgc tcatcaatgg tacccatatg atggaggcaa
      601 tcgcgatcct ggcaacgcac gatgcgcagg tactcatacg tgcggcggag gtcgcctgcg
      661 ccatgagcat cgaggggctg atgggcagcc atgcgccact ggatgcgcgc atccataagc
      721 gtagaggaca acacggtcag cagatcagcg cggcgcgcct gcgccagctg gtcgagaaaa
      781 gcgagatcaa cgtcagtcac gccgattgcc cacgcgtaca ggacccctac tcgatgcgct
      841 gcgccccgca ggttctcgga gcggtacgcg atgccctgga tgtctgctcc ggtatcttcg
      901 agcgcgaact ggatgccgtc accgataacc cgctcatctt tcccgacgac ggcgacgtgt
      961 tgagcggcgg caattttcat ggccagcccc tggcgctggc gctggacgtg ctggcgatcg
     1021 ccctggcgca actggccagc ttttcagagc gccgcatcta taatctgatg ggaccgcacg
     1081 attgggacga aggcggcgcg ccgctcttct taacgcccaa tcccggcttg aactccggcc
     1141 ttatgatcgt gcaatacgtg gcagcctcgc tggtcaatga aatcaaaata ctggcgcatc
     1201 ccgccagcat tgactcaatt cccacctcgg ctggtatgga agatttcaac agcatgggcg
     1261 cgaccagcgc tcacaaactg ctgcgggtcc tcgaacaggc ccgccaggta gtcgccatcg
     1321 aactgatgtg cgcagcgcag atgctggagt tccgcaagcc actcaagccc ggcctcggtg
     1381 tcctggccgc ctacgacatc gtgcgctccc acgtaaccag actggaagag gaccgtgtac
     1441 ttacccccga catcgattcg ctgacaaaag ccatctatga gggcgcattc gaatcgcttg
     1501 cgtaatgttt cacgtgaaac attacgcaag ctgatacaag agggcgaccg caagagtacg
     1561 cccttgtcat gctgagcgca gcgaagcatc tgcttgatgg gccgagatgc ttcgctgcgc
     1621 tcagcatgac aagggaggct acttcggtcc ttcggttgat gctgcttggc gtacccttgc
     1681 ggtcgccctc gaagtagatg tgttgtccag cttgcagctt attccataca tgtgctacac
     1741 tccttactgg aactggtcaa cggtgactgt gttcccgctt cgattgatgt gtgcgaagca
     1801 gccgaaggag taacgatgat gattacccat gatttgccac caggtatgca cggttctttt
     1861 cccgacccat cactttcaca cttaccatcc gatcaaattg agcaagtacg ggcgctgcgc
     1921 agcgaagtag tggcgcgtta tcgccctcat attctcgaag gaccagggcc aatccgtatt
     1981 atggcgcgcc tggtggcgga gctagaacag ctatgccggg aacatgccat cagcgacgaa
     2041 gtcatccagg ccagtattgt gaaccgcacc atcggcgacg tagacctgcg gcgcgttacc
     2101 gagtgcgaag gtgaagcagg cggaccgggc gattttcgcc tgctggcgga cgaactcggc
     2161 atcgcgctgc ccggcgagca attgcctggc tatactgcga gtggggaaac atatctctgg
     2221 ctgcgcgagc aaatgcaggt acttgaacgc accttattga agcagcgcat cgacgtgcgt
     2281 atgtacgata tattcggcat cggtaatccg ctcctgcgcg gctggctggc cagcatgatg
     2341 cagcagcagt ggggactgcc cttcacgaaa gagcagattc atctgggcat aggcgcgatg
     2401 gattgcgtcg ataaatcgct gcgctcgctg cgcttcgtcc tcatggaaca aacaggtacc
     2461 aaccctgcta tcctgtttcc tgaacccggt ttcaacgtcc ccgaatggca ggcgcgctcc
     2521 tatggctacc gcatgcagca ttacaaaacc gatgcctcgc gcgccttcaa attaccggcg
     2581 gcgcaactgg aagaaatcct gcaaaacacg ccggatatcg gcgtgattta cctgactgtg
     2641 accaacaacc cgaccacttt tgcatacaac gcccaggaac tcgacgaact cttcgccgct
     2701 atacgtcctt attgggaaca gggacgccac atctacatac tggctgacct ggcctacgtc
     2761 ggcacgggcg atcctgcgga cgacgccgcc cgcatggcca cctttgccac gcccgacgtg
     2821 atgcggcata ctatcttcgt caacagcttc tccaaaacgc tctcgctaac cggcgatcgc
     2881 tttggttacg tcgccatcgg cgatcccaag ctcgcgcccg ccctgggacc cggttggaca
     2941 aacagcacgg cggcgctgcc cggcgaatgg caactacgct acatggccgt cgtgcgcctc
     3001 ctacaggaac gcccctggct gatcgcaaaa ctgcgcgcgc tctatcgctt acgtcgcaga
     3061 cgctttgaaa accagctaca tcacatcaat atggaacagc ctctcttcca gcacatctac
     3121 gagggtgatg atgccaccgt ctacacctgg tgccagcttg cccacggcga agatgccttc
     3181 tcgcttttcg agaaaaccgg catcgccggt gtccctggct ccggcttcgg ctacaccgac
     3241 gactttatcc gcttctcgat cggcgtcatt cccgtgccgg aatggtgact ccgctctttt
     3301 ccgcctcc
//
LOCUS       DASZAK010000956         2253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_273697, whole genome shotgun sequence.
ACCESSION   DASZAK010000956 DASZAK010000000
VERSION     DASZAK010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273697"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VGT44_16740"
     CDS             <1..1484
                     /locus_tag="VGT44_16740"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582507.1"
                     /translation="YGVCNLGALNLSAFVVDGKMDYSRLAEMSRVAMRFLDNVVDANE
                     YFIEENRVAQLGTRRTGLGTMGLADALIKMEIPYGSEESVPVIERIYATIRDAAYEAS
                     ADIATEKGAFSHFERDKYMKGEFIRQLPKAIQEKIKKQGIRNAVLLTQAPTGTTSLFS
                     GVSSGIEPVYDFAMVRRDRTGEHILYHPLLQAWREAHPNEPTPKYFVSSNDLTPEEHV
                     RVQATIQRYTDSSISKTVNAPNNHTVEQVQKLYRLAYETGCKGVTYYRDGSRDAVLTR
                     VADEKKAEQPEQQTVKVEPVTSIQDGVKPRPAVVHGYTRQVNAPEGKINVTINSDEHG
                     PLEVFVNVGKAGSDIAALAEALGRLISLNLRVLSPLSQTDRAKEIADQLRSIGGSRSV
                     GFGMQQVRSLPDAVARALELHFEAMEREKAQTPAPEAHAAPEVPPIANGKDASSANGN
                     HIDSPLGMTRMSITGNLCPQCGCNTMVYEEGCKKCYNCGHSEC"
     gene            1753..>2253
                     /locus_tag="VGT44_16745"
     CDS             1753..>2253
                     /locus_tag="VGT44_16745"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2582508.1"
                     /translation="MFHPQEITRIGQEINSEQTLHSIIDKTRNAAKADIIVLYPYETA
                     LRRFIFPPLIAGELLDSPVQPLSLGLLDDSAERVALIKDSHFAKDCATFYKTLQGSAH
                     SLVGSFSQREKVRSIAAVPLQVGDELVGVLFINFRQPQRFDAAQKLFIDGLAQYAAIA
                     IKNMRTL"
BASE COUNT          544 a          635 c          624 g          450 t
ORIGIN      
        1 cttacggcgt gtgcaacctc ggcgcgctca atctctcagc tttcgtcgtc gatggcaaga
       61 tggattactc gcgcctcgcc gagatgagca gggtggcgat gcgcttcctc gacaatgtcg
      121 tggatgccaa tgagtacttc atcgaggaga atcgcgtggc gcagctcggg acgcggcgca
      181 ccgggttggg aacgatgggg ctggccgacg cgctgatcaa gatggaaatt ccctacggca
      241 gcgaggagtc ggtgccggtg atcgagcgca tctatgccac cattcgcgac gcggcctatg
      301 aagcttccgc cgatattgcc accgagaagg gcgcgttctc gcacttcgag cgcgataaat
      361 atatgaaggg cgagttcatc aggcagttgc caaaggccat tcaggagaag atcaagaagc
      421 agggcattcg caacgccgtc ttgctgacgc aggcgccaac cggcacgacc agcctgttct
      481 ccggcgtcag ttctggcatc gagccggtct acgacttcgc catggtgcgg cgcgaccgca
      541 cgggcgagca tatcctgtac cacccgctgc tccaggcatg gcgcgaggcg catcccaatg
      601 agccgacgcc gaaatacttc gtctcgtcca acgacctgac gccggaagaa cacgtgcgtg
      661 tgcaggccac tatccagcgc tacaccgatt ccagcatcag caagacggtg aacgcgccca
      721 ataaccacac cgttgaacag gtacagaagc tctaccgcct ggcctatgag acgggctgca
      781 aaggagtgac gtactaccgc gacggctcgc gtgacgcagt tctcacgcgg gttgcagacg
      841 agaagaaggc cgaacagcca gagcaacaaa ccgtcaaggt tgagccggtg acatccatcc
      901 aggacggcgt caagccgcgc ccggcggtgg tgcatggcta cacgcgccag gtgaacgcgc
      961 cagagggcaa gatcaacgta acgatcaaca gcgacgagca tgggcctctg gaggtgttcg
     1021 tcaacgttgg caaggcgggc agcgatatcg cggcgctggc cgaggcgctg ggccgcctga
     1081 tctcattgaa cctgcgcgtc ctcagccccc tctcgcagac cgaccgcgcg aaggaaatcg
     1141 cggaccagct gcgcagtatc ggcggcagcc gctccgtcgg cttcggcatg cagcaggtgc
     1201 gctcgctgcc cgacgctgta gctcgtgcgc tggaactgca cttcgaggcc atggagcgag
     1261 aaaaggcgca aacaccagcg ccggaagcgc acgccgcacc ggaggttccg cccatcgcga
     1321 acggcaagga tgccagcagc gccaacggca atcacatcga tagcccgctc ggcatgacac
     1381 gcatgagcat caccggcaac ctctgcccgc aatgcggctg caacacgatg gtctacgagg
     1441 aaggctgcaa aaagtgctac aactgcgggc atagcgagtg ttaagagcgg tgtagctgac
     1501 gcggcctgta gaggcgatgc agcccgaaat agacggcgat tgggaaagga cacgtgtcct
     1561 ttccctttta ctctcattcg actcgagaag cccataagac aaatttttgt gtctctccct
     1621 cccaataata tattgagaca ggaggaacta ttttatgata tactatagac ctgaataaac
     1681 ttaatatccg ctaaatttat gataagacaa tctgtgatgt gaactagcgt cacttgtaga
     1741 aggtgcagca aaatgtttca ccctcaagaa attacccgta ttggacaaga aatcaactca
     1801 gaacaaacat tacactcgat tattgacaaa acccgaaatg ctgccaaggc tgatattatt
     1861 gtcctctacc cctatgaaac tgctcttcga cgctttatat ttcctccact tatcgccggt
     1921 gaattactcg actcacccgt tcagccttta tccctcggcc tattagatga ttcagcggaa
     1981 cgcgtagcgc ttattaagga ctctcatttt gctaaagatt gtgctacctt ttataaaacg
     2041 ctgcagggca gtgcccatag cttggtggga agttttagtc agcgagagaa agtgcgctcc
     2101 attgcagctg ttccattgca ggtcggagac gagttggttg gtgtgttgtt tatcaatttt
     2161 cgtcaaccac aacgttttga tgccgcacag aagctgttca tagatggatt agcccaatac
     2221 gcagctatcg ctattaaaaa catgcggaca tta
//
LOCUS       DASZAK010000957         2495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_452156, whole genome shotgun sequence.
ACCESSION   DASZAK010000957 DASZAK010000000
VERSION     DASZAK010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452156"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VGT44_16750"
     CDS             complement(<1..551)
                     /locus_tag="VGT44_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2582509.1"
                     /translation="MTERLRVLIVDDQRLRCEGFRKLIELEPDLEVVGMAGDGEEALA
                     MVERLSATRTAPDVVLMDVRMPRLDGIRATRAFKERWPEIRVVILTTFDDRELIQAGL
                     QAGARGYVLKDITAEQLATTIRVVAQGQVLLHPDVAHKVLASFSSAPIEPAMIAPATP
                     AFVGGSDLAQLTEREREILALLAR"
     gene            complement(548..1834)
                     /locus_tag="VGT44_16755"
     CDS             complement(548..1834)
                     /locus_tag="VGT44_16755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2582510.1"
                     /translation="MKFKQEQIIQTLSMPLLGWYLRWIPWVITVFVCAGYLSGVLTTQ
                     PLTLLSLLLLTGTYGAWLVIYHIGARQFKVRAHPGFVLALFCLACASQFIPLPGTSTD
                     WLPLLPLITAGLMATVKPRSLSLLAAGALCLSSYLAIHLIVPDWNDVSGQVRLLLSFL
                     SLYGYAAILLELVAAHTALEGSNARLAAAHTRLQEYSTQAEELSAVRERNRIAREIHD
                     TLGHALSLLSVQLETATQLEARGDPRLHEELLQARQVVKACLTEVRHSVEALRPGDAS
                     PGSLQEQLRKLVAEFETTCRETRITLDLEEATHPLNPEVCLTLYRCAQEALTNIRKHA
                     HATKVLLRLSTSDEQVELTVLDNGQGCPPEHEQRAPVFGLQGMRERVALLDGTLRAGP
                     QPGHGWRVEVVLPLKMRGQAESSATGGRETREKVER"
     gene            2076..>2495
                     /locus_tag="VGT44_16760"
     CDS             2076..>2495
                     /locus_tag="VGT44_16760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582511.1"
                     /translation="MPKKTVVTPEREMSAQNLPKQGHTSVAVSSGGAAAQNTLRNIRL
                     IIGREYKNRVTQRVFIITSIILLAIVFLAPFIPTIVKLVQSITARPGSQTQVVVVNNA
                     GTVAGLNEGMLISYISSVLNGTTTTSPAPYAITSQPQA"
BASE COUNT          489 a          756 c          770 g          480 t
ORIGIN      
        1 cgcgccagca aggccaggat ctcccgctcg cgctcggtca actgcgccag gtcgctcccg
       61 ccgacgaacg caggagtggc gggagcgatc attgcgggct cgatgggtgc agaagagaag
      121 gaggcaagga ctttgtgcgc aacatcggga tgcaggagga cctgcccctg tgcgaccacg
      181 cgaatggtcg tagcgagctg ctcggcggtg atgtctttga gcacatagcc ccgggctccc
      241 gcttgcagac ccgcctggat cagctctcga tcgtcgaagg tcgtcaggat gaccacgcgg
      301 atctcgggcc agcgctcttt gaaggcacgc gtcgcgcgaa tgccatccag acgcggcatg
      361 cgcacatcca tcagcaccac atcgggtgcc gttcgcgtgg cagagagacg ttcaaccatg
      421 gccagcgctt cctcgccatc ccccgccatg ccaaccacct ccaggtccgg ctccagctca
      481 atcagcttgc gaaagccctc gcaccgaagg cgctggtcat ctacaatcag cacgcggaga
      541 cgctctgtca tcgctcgacc ttctccctgg tttcgcgtcc tcccgtggcg ctgctctccg
      601 cttgtccccg catcttgagc gggagcacca cttcgacgcg ccagccgtgc ccgggttggg
      661 ggccagcccg tagcgtccca tcaagcagcg cgacgcgctc gcgcatgccc tgcaggccga
      721 agacaggcgc tctttgctcg tgctccggcg ggcacccctg gccgttgtcc agcacggtca
      781 gttccacctg ctcatcgctc gtggagagcc gcagcagcac cttggtggcg tgcgcatgct
      841 tgcgaatatt ggtcagcgcc tcctgcgcgc agcggtagag cgtcaggcac acttctgggt
      901 tcagagggtg cgttgcctct tccagatcga gcgtgatcct ggtctcgcgg caggtggttt
      961 caaattccgc caccagcttc cgcagttgct cctgcaacga acctggggag gcatcgccgg
     1021 gtcggagcgc ctcgacggag tgacggactt ccgtcaggca ggctttcacc acctgtcgtg
     1081 cctgcagcag ttcctcgtgc agacggggat cgccgcgcgc ttccagctgc gtggccgttt
     1141 ccagctggac cgagaggagc gacagggcgt gaccaagcgt gtcgtggatc tcacgggcaa
     1201 tccggttgcg ctcgcggaca gccgagagtt cctccgcctg ggtgctatac tcctgcaggc
     1261 gcgtgtgggc cgctgcgagc ctggcgttgg agccttccaa cgccgtgtgg gccgccacga
     1321 gttcaaggag gatagccgcg tatccataga ggctgaggaa cgagagtagg aggcgcacct
     1381 ggccgctgac atcattccag tccgggacga tgaggtggat cgcgaggtag gaactcaagc
     1441 agagggcgcc tgccgcgagc agactgagag accgtggttt gaccgtcgcc atcaagcccg
     1501 ctgtgatgag cggcagcagt gggagccaat cggtgctcgt gcccggcaag gggatgaatt
     1561 gggaggcgca agccaggcag aacaaggcca gcacgaagcc tggatgtgcg cggaccttga
     1621 actgccgtgc cccgatatga tagatcacga gccaggcccc atacgtcccc gtgagcagaa
     1681 gcaagctgag cagggtgaga ggctgcgtcg tcaagacccc ggagaggtag cccgcgcaga
     1741 cgaaaacagt gatgacccag ggaatccacc gcaggtacca gccgagtaat ggcatggata
     1801 gtgtttgaat gatctgctct tgcttgaatt tcatgagtgt acccgccggc tgtgagagat
     1861 gcacttcttt tccaaagtat acctcactgg ccttcccact cgccagtgtc tgcagtcatg
     1921 ggtgtgctcg acaggaccta tgacgctggt catagggtcc atggtcagaa ataggcccgt
     1981 gcttcgtgac tgatgggact tgtgtcgctc tgcttatgct gactacactt agcacgagga
     2041 atgaagcttg ttcatactct gagaggaatg acaccatgcc aaagaaaaca gttgtcacgc
     2101 cagaaagaga gatgtcagca caaaatttgc cgaaacaggg gcatacatct gtggccgtca
     2161 gctcaggcgg ggccgctgcc cagaacaccc tgcgcaatat ccggctgatt atcgggcgcg
     2221 aatataaaaa ccgggtgacc cagcgcgtgt ttatcatcac gagcattatc ctcctggcga
     2281 tcgtgttcct tgcccctttt atcccaacca ttgtgaagtt ggtgcagtct attactgctc
     2341 gtccaggttc gcaaacccag gtcgtggtgg tgaataacgc gggtacggtt gcgggcctga
     2401 atgaggggat gttgatctcg tatatcagtt cggttctgaa cgggacgaca accaccagcc
     2461 ctgctcctta tgcgattacc agccagccac aagct
//
LOCUS       DASZAK010000958         5848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_380658, whole genome shotgun sequence.
ACCESSION   DASZAK010000958 DASZAK010000000
VERSION     DASZAK010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380658"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VGT44_16765"
     CDS             complement(<1..1002)
                     /locus_tag="VGT44_16765"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEV2582512.1"
                     /translation="MLRSSLLCETVKLAIDDRLVILNSAADPFVQESALRLAAGELVL
                     AEDNLAALQRAHEIILRERVPISPRHIPFHEYTLHMPPATMDIAVMNILYQPSKAWID
                     YALQLAAYTLKPGGQFFVQGAKDRGILTLAKRMGEMFGGVETLEIHKGERVVRSICNL
                     EPGGSDALPVLTPFAEGKLDEGTRLLLEALEVHKTDEALDIGCGAGYIGCHVASRAVK
                     GKVTMVDASLAAVDAARRMIEQRGLPNARALASDGTQAVREERYDLVVTNPPFHIGGI
                     QTTEIGHRFIREAALVLHPRGRFYLVANRFLKYETTMRECFSTVEEVGGNTRFKVLRG
                     "
     gene            1008..1685
                     /locus_tag="VGT44_16770"
     CDS             1008..1685
                     /locus_tag="VGT44_16770"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEV2582513.1"
                     /translation="MAFYIDSAFLHDIMNAAQTVPVAGVTTNPSILLAAQERGQLLSP
                     DELLDELLRKVAGYIFMQPGATEDEQMYAQALSYIEADPRRVIPKIPMTNAGMRVALR
                     LKAQQQRVAFTAVTTVAQAYSAALISADYVIPYYNRMERSGLDAAQRISEIADTLHNV
                     QATSARPTRILTASIKTADEAASALLAGSDDLTVNPEVLLNLLTDPLSEEAIARFTQD
                     WQKLKKL"
     gene            complement(2053..2829)
                     /locus_tag="VGT44_16775"
     CDS             complement(2053..2829)
                     /locus_tag="VGT44_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582514.1"
                     /translation="MQNMQTKKKPVAPAVMDIDLEDIGEEDEYYLKRTRPHTSIRRYD
                     LPPLRDAMEEPDTETGTMIRRRRSAMKEDNQVTSGRFTSKATIPTVAKTPKIKKPASI
                     GGQGLHIKRLPLIAVLLGMVVTAALVVSFSAISNWWQVHQDDVTYSRPRTFQIDAVVG
                     HNDSPANPSHFIFINLNRHVVIIEMPGGDAAHARIYSGPTLFGNGQDLTPITAEFRDV
                     NGDGKLDMIVMIQDQRLVYINTGTAFRPLQPGEQVNLPSS"
     gene            3019..4410
                     /locus_tag="VGT44_16780"
     CDS             3019..4410
                     /locus_tag="VGT44_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2582515.1"
                     /translation="MLDTILMLAEAFQARQKQLYMVGGTVRDVLLHRGKSNDADLATD
                     ARPDEIKQIVAPTRPNAVILVGERFGTVRLHYDTDIIEITTFRSERYNPESRKPEVCF
                     GTRLEDDLLRRDFTINAMARHPLTGEIIDLFGGRQDLEAHILRAVGNEPDKRFDEDPL
                     RLLRAVRFAAQLDFAIESHTRISIMRQASTLQKISRERIRDEMNKLLLSNHPLKGLNL
                     LVELELMQWIIPEVLELIGVSQLPQQPPSQRAARSKDVYAHVLRVVERSSPRLACRWS
                     ALLHDIAKPRTRTVEDGKVHFFGHEDVGARMAYDILKHLHFDRDFIASVSNIVRLHMR
                     ANAYTSDWTDGAVRRLMLDSGDDLADLLDLSRADITSYRVDKVERAAARVNELAERCQ
                     RLKEEAERVPLKSPLDGRELMALFGRPPGPWLRPVKEHLLGLVIDGALSPDDKAGATE
                     IARAFVATTESVV"
     gene            4504..5259
                     /locus_tag="VGT44_16785"
     CDS             4504..5259
                     /locus_tag="VGT44_16785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV2582516.1"
                     /translation="MRDKIVHYLNEYLQIDQFRDYGPNGMQVIGRPDVKRVALAVSAN
                     LECFRLAAEQQADMLIAHHGLFWENMPREIGAMMKARLKLLFEHDMTLLGYHLPLDAH
                     PLSGNNALWLARLGFDLESIELANFRGKAIGATGISPKRQTLRDLVEQIASIAGATPH
                     VYAYGPQTIRRLGVVTGGGQNNLLEAIARGCDTFLTGEVGEPTEGIAREGGVNFIAAG
                     HYNSEKIGVQALGDLLKAQFDVETFFCDVPNAI"
     gene            5302..5838
                     /locus_tag="VGT44_16790"
     CDS             5302..5838
                     /locus_tag="VGT44_16790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582517.1"
                     /translation="MTKANKPGPGSQKPASGQGKAGDIQKKTASTSATAPAKTAPPTP
                     PTPPAHRGKSGGKYRKSTIGGTAVTGSKSTIPKEIPTSGPAQDRPEMYNRDSRRRMQH
                     MGTGPYNDTTPVPARKRYEKKVEERKKRQEAVKKTVVTKGPSTNVKIGNKNTYFLLTV
                     LGIIVLIIVIALIVRHPF"
BASE COUNT         1309 a         1761 c         1644 g         1134 t
ORIGIN      
        1 tccccgcaat accttgaagc gtgtattgcc gcctacctcc tccaccgtgc tgaagcattc
       61 gcgcatggtc gtttcgtatt tcaagaagcg attggccacc aggtagaagc gtccgcgtgg
      121 gtgcaggacg agcgcagcct cgcgtataaa acggtggccg atctcggtgg tctggatgcc
      181 gccgatgtgg aaaggaggat tggttacgac caggtcatag cgctcctctc gcacggcctg
      241 tgtgccatcg ctggcaagcg cgcgcgcgtt gggcagccca cgctgctcga tcatgcgccg
      301 cgccgcatca acggcggcaa ggctggcatc caccatcgtg actttaccct tcacagcgcg
      361 gctggcgacg tggcagccga tgtaacccgc gccgcagccg atatccagcg cttcgtcggt
      421 cttatgcacc tccagcgctt ccagcaacag ccgcgtcccc tcgtccagct tgccctcggc
      481 aaacggcgtg agcaccggca aagcatccga tccgcctggc tcaaggttac atatggagcg
      541 cacgacccgc tcgcctttat ggatttccag cgtctcaacc ccgccgaaca tctcacccat
      601 gcgcttcgcc agcgtcagaa taccgcgatc tttcgcgccc tgcacaaaga actggccgcc
      661 gggcttgagc gtataggcag ccagttgcag cgcataatcg atccaggctt tgctcggctg
      721 gtaaagtata ttcatcactg cgatatccat agtcgcgggc ggcatgtgca gtgtatactc
      781 atggaaggga atatggcggg gcgagattgg tacgcgttcg cgcaaaatga tctcgtgcgc
      841 ccgttgcagc gcggcgaggt tgtcctcggc cagcacgagt tcgcccgccg cgagacgcag
      901 cgcggactcc tgcacgaacg ggtccgccgc ggagttgagt atcacgagcc ggtcatcgat
      961 ggccagcttc acggtttcac acaataacga agaacggagc atgatctatg gctttctata
     1021 ttgatagcgc tttccttcat gatatcatga acgcagcgca aacggtaccg gtagcgggcg
     1081 tgacgaccaa cccctctatc ttgctggcgg cccaggagcg cggccaactg ctcagccctg
     1141 atgagctgct ggacgagctg ctgcgcaaag tagcgggcta tatcttcatg cagccaggcg
     1201 cgaccgaaga tgagcaaatg tacgcgcagg cactatccta tatcgaggcc gaccccaggc
     1261 gcgtcatccc caaaatacct atgaccaatg ccgggatgcg tgtcgcgctc cgcctgaaag
     1321 cgcagcagca gcgcgtggct ttcaccgccg tcacaacagt cgcccaggca tacagcgccg
     1381 ccttgatttc cgccgactat gtgatcccct actacaaccg catggagcga tccggcctcg
     1441 atgccgccca gcgcatctcc gaaatagctg acacactgca taacgtacag gcaacatccg
     1501 ccaggccaac gcgtatcctc accgccagca ttaaaaccgc agatgaagcg gccagcgcgc
     1561 tcctcgccgg gtctgatgat ttgaccgtca atcccgaagt cctgctcaac ctgctcaccg
     1621 atcccttgag tgaagaggct atcgcacgct ttacgcaaga ttggcaaaag ctgaaaaaac
     1681 tgtaatgtgg cgattcgctg taacagcgac aaatatgaac aaattgtcag gaaaaagtgt
     1741 ccggttttca tagcatcgta agataaacat ggtatatttt gtctcgtgag cagaagagat
     1801 aagaaccgaa gacggtgacc ggagcggcag tggatgggag agtggggaaa gtattctgcc
     1861 gggtgggtta tgtggcaagg gctatgaaaa tcggcaggca tcaacgttac gtatcgcaca
     1921 ggtgcgcggt gacaaagtgg cagggagcaa tagcgataca atgcgcgcca acccgcgcac
     1981 agggggaggg gatacgtagg cagggtggaa gcctgaaggg ggggatcaaa gccttcaggc
     2041 tttttttcat gcctacgacg atggcaaatt cacctgctcg ccgggttgaa gtggtcgaaa
     2101 cgctgtgccg gtattgatat agaccagccg ttgatcctgg atcatcacga tcatatccag
     2161 ttttccatcc ccgttgacat ccctaaactc tgccgtgata ggcgtcaggt cctggccatt
     2221 gccaaaaagt gtaggaccac tgtagatacg cgcgtgcgcc gcatctccgc ccggcatctc
     2281 aataattaca acgtggcggt taaggttgat aaagataaag tgtgaagggt tggcaggact
     2341 gtcgttatgc ccgaccaccg cgtcaatctg gaacgtcctg ggccgactat acgtaacgtc
     2401 gtcctggtga acctgccacc agttgctaat cgcgctaaag ctcaccacca gcgccgccgt
     2461 cacaaccatg cccagcagca ccgcgataag cggcagcctc ttaatatgta acccttgccc
     2521 gccaatcgat gcgggtttct ttattttcgg cgttttcgct acagtcggaa tggtcgcctt
     2581 cgacgtgaac ctgccacttg tgacctggtt atcctccttc atcgccgaac gccggcggcg
     2641 aatcatcgtc cccgtctccg tgtcaggttc ctccatcgcg tcacgcagcg gcgggagatc
     2701 atagcgccgg atgctggtat gtggccgcgt ccgctttaag taatactcat cctcttcacc
     2761 aatatcctca agatcaatat ccatgacggc aggcgcgacc ggcttctttt tcgtctgcat
     2821 attttgcatg gcgtcctatg cctccttgcg aagtagtgac tagataattt gttctagtac
     2881 ttgtattata cccacttctg caaggatgtc aagggttttt agaacttccg tgcgaaattc
     2941 gatggctagc caagcctcgc cagccatgat acaatagacc cattacagaa taccgaaatg
     3001 ggaacccatt cttaaactat gctcgacaca atactcatgc tggcggaagc gtttcaggcc
     3061 aggcagaaac aactctatat ggtcggggga accgtgcgcg atgtcctgct gcatcgcgga
     3121 aaatccaacg atgccgacct ggcgacggac gcgcggcccg acgagatcaa gcagatcgtg
     3181 gcccccacgc gcccaaatgc cgtaatcctc gttggtgagc gctttggcac ggtgcgcttg
     3241 cactacgata ccgacatcat cgagatcacg acctttcgca gcgagcgcta caatcctgag
     3301 tcgcgcaagc cggaggtctg ttttggcacg aggctcgaag acgacctgct gcggcgcgac
     3361 tttaccatca acgccatggc gcgccatcca ctgacagggg agatcatcga cctgtttggc
     3421 ggcaggcagg accttgaagc gcatatcctg cgcgcagtcg gcaacgagcc ggataagcgc
     3481 ttcgacgaag accccctgcg tctgctgcgc gccgtgcgct tcgccgcgca actcgatttc
     3541 gccatcgagt cgcacacgcg catctcgatc atgcgccagg cgtccaccct gcaaaaaata
     3601 tcgcgcgagc gtatccgcga cgagatgaac aagctgctgc tctcgaacca tcccctcaaa
     3661 gggctgaatt tgctggtcga actggaactt atgcagtgga tcatcccgga ggtgctggaa
     3721 ttaatcggcg tcagccagtt gcctcaacaa ccaccgtcgc aacgcgccgc gcgctccaaa
     3781 gatgtctacg cccacgtctt gcgcgtcgtg gagcgttctt cgcctcgcct cgcctgccgt
     3841 tggagcgcgc tgctgcacga catcgccaag ccgcgcacgc gcaccgtcga ggacggcaag
     3901 gtgcatttct tcggacacga agatgtcggc gcgcgcatgg cctacgatat tttgaaacac
     3961 ctgcactttg accgcgactt catcgcaagc gtttcaaaca tcgtgcgttt gcatatgcgc
     4021 gccaatgcct atacctccga ttggacggat ggagcggtgc gccgcttgat gctcgacagc
     4081 ggggacgacc ttgccgacct gctcgatctc tcgcgcgccg acatcaccag ctaccgcgtc
     4141 gacaaagtcg agcgggcagc cgcgcgcgtc aacgaactcg ccgagcgctg ccagcgcttg
     4201 aaagaggagg ccgagcgcgt accgctcaag agtccactgg atggcagaga gctgatggcg
     4261 cttttcgggc gaccgccagg accctggctg cgtcccgtca aagaacacct gctgggcctc
     4321 gtgatcgatg gcgcgctttc gcccgacgac aaagccgggg ccaccgaaat cgccagagcc
     4381 tttgtagcaa ctacagaaag cgtcgtatga tcgctgtaca tgtgcgtgtt gagcatggcg
     4441 accgcaaggg tcgccatgct caacatacgc tagcgaaaac atacggcgcg agaggataac
     4501 gcaatgcggg acaaaatcgt ccactacctg aacgaatatt tacaaatcga ccagtttcga
     4561 gactatggcc cgaacggaat gcaggtgatt ggccgccctg atgtgaagcg cgtcgcactg
     4621 gcggtcagcg cgaacctgga atgtttccgg ctggcggcgg aacagcaggc cgatatgctg
     4681 atcgcgcatc atgggctgtt ctgggagaat atgccgcgcg agatcggcgc gatgatgaaa
     4741 gcgcgactca agctgctgtt tgaacacgac atgacgctgc tcggctatca cctgccgctg
     4801 gacgcgcatc cgctatcagg taataacgcg ctctggctgg cgcgcctggg tttcgacctg
     4861 gaaagcattg aactggctaa cttccgtggt aaagcgattg gcgctaccgg catctcgccg
     4921 aaacgccaga ccttgcgcga cctggtggaa cagatcgcct cgatcgcggg cgcgaccccg
     4981 catgtctatg cctatgggcc gcaaacgatc cgccgcctgg gcgttgtgac cggtggcggc
     5041 caaaataacc tgcttgaggc catcgcgcgc ggctgcgata ccttcctgac gggcgaggtc
     5101 ggcgagccga ccgagggcat cgcgcgcgaa ggcggcgtca atttcattgc ggctggccat
     5161 tataattcgg agaagattgg cgtacaggcg ctgggagacc tcctgaaggc gcagttcgac
     5221 gtagagacgt ttttttgtga cgtacccaat gctatctaac cgtcgtaggg acgcgatttc
     5281 atcgaagttg aggacaccat catgacgaaa gcaaataaac caggcccagg ctcacagaaa
     5341 cccgcctccg ggcagggcaa ggcaggcgac attcagaaga agaccgcctc taccagtgcg
     5401 actgccccgg caaaaaccgc gccgccaaca ccgccaacac cacccgcgca ccggggcaaa
     5461 tcgggcggga agtaccgcaa atcgacgatc ggcggcacgg cggtgaccgg atcgaaatcg
     5521 accattccga aggaaatccc gaccagcgga cccgcgcagg atcggcctga gatgtataac
     5581 cgcgactcgc gccgccgtat gcagcatatg ggaaccggcc cctacaacga cacgacgccc
     5641 gttccggcac gcaaacgcta cgagaagaag gtcgaggagc gcaagaaacg ccaggaagcg
     5701 gtcaaaaaga ccgtcgtcac aaaggggcca agcaccaacg tcaaaattgg caacaagaac
     5761 acctacttct tgctgaccgt tctcggcatc atcgtcttga ttatcgttat cgcgctgatc
     5821 gtccgtcacc cgttctaagc gcgtagtc
//
LOCUS       DASZAK010000959         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_166769, whole genome shotgun sequence.
ACCESSION   DASZAK010000959 DASZAK010000000
VERSION     DASZAK010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166769"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            140..>2022
                     /gene="nifJ"
                     /locus_tag="VGT44_16795"
     CDS             140..>2022
                     /gene="nifJ"
                     /locus_tag="VGT44_16795"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEV2582518.1"
                     /translation="MDSTWITVEGNEAVANVAHMLSEVIAIYPITPSTLMGELADAWS
                     AAGRANLWGTVPLVIEMQSEGGAAGALHGALQTGSLVTTFTASQGLLLMLPNMYKIAG
                     ELTPAVFHVAARSLAAQALSIFGDHADVMAARATGFALLASNSAQEAQDMALIAHAAT
                     LQARVPFLHFFDGFRTSHEIDKIKLLEVAQIRSMIDDSLVQAHRERGLSPDHPFIRGT
                     AQNPDVYFQARESVNPYYLACPAIVQKTMDIFGQMTGRHYHLFDYAGAPDAERVIVVM
                     GSGAETVQATVEYLAARGEKVGVLKTRLFRPFSVEHCLQALPSTVKVIAVLDRTKEPG
                     SAGEPLYLDVTAAISEGWRTMHPQTYPQIIGGRYGLSSKEFTPAMVKGIFDEMSASAP
                     RQHFTVGINDDVTHSSLAYDPAFSIEPQETVQCVFWGLGSDGTVGANKNSIKIIGEET
                     DNDAQGYFVYDSKKAGTVTISHLRFGPHPIHAPYQIGAGEAHFVACHQFTFLERYDVL
                     KYARPGAVFLLDSLYSPEEVWEHLPGETQQAIRDKRLRFYVINAHKVASEIGMGGRIN
                     TIMQTCFFAISGILPREEAIEQIKKSIKKTYGKRGEAVVQRNYQAVDATLQHLHEALI
                     PAET"
BASE COUNT          426 a          610 c          607 g          379 t
ORIGIN      
        1 gtcctgtgaa caaaatgtga catggacatt accggctgtg atcgaactgt gatacctcgg
       61 cagtatgctt ccccctagag acgctagcga gagtataaaa gcaattacga tgagatctga
      121 atgatcgttg gaggcacata tggacagcac ctggattacg gttgagggca acgaagctgt
      181 tgccaatgtg gcgcatatgc tgagcgaagt gatcgccatt tatccgatca cgccctcgac
      241 gctgatgggc gaactggccg atgcgtggtc ggcggcgggc cgcgctaacc tgtgggggac
      301 ggttcccctg gtgattgaga tgcaatcgga agggggagcc gccggagctt tgcacggcgc
      361 attgcagacg ggatcactgg taacgacctt cacggcctcg cagggcctgc tgttgatgct
      421 gcccaatatg tataagatcg ccggcgaact gacgcccgcc gtcttccacg tcgcggcgcg
      481 ctcgctggcg gcgcaggcgc tctctatctt cggcgaccat gcggatgtga tggcggcgcg
      541 cgccaccggt tttgccctgc tggcatcaaa ttcggcgcag gaggcgcagg atatggctct
      601 gatcgcgcac gcggccaccc tgcaagcgcg cgtgcctttc ctgcacttct tcgatggttt
      661 tcgcacctca cacgaaattg acaagatcaa gctcctcgaa gtggcacaga ttcgcagcat
      721 gatcgatgat agcctcgtgc aggcacaccg agagcgtggg ctttcgcccg atcatccctt
      781 cattcgcggc accgcacaga acccggacgt gtacttccag gcgcgtgaaa gcgtgaaccc
      841 ctactacctg gcctgtcccg ccattgtgca aaagacgatg gacatttttg ggcagatgac
      901 cgggcgccac tatcatctct tcgattacgc gggcgcgccc gacgcggagc gtgtgatcgt
      961 ggtcatgggt tcgggggccg aaacggtaca ggcaacggtc gagtacctgg cggcgcgtgg
     1021 cgaaaaggtg ggcgtgctca agacgcgcct gttccgccca ttctcggtgg aacactgctt
     1081 gcaggcgctg ccatcgaccg tcaaggtcat cgccgtactt gaccgcacga aagagccggg
     1141 cagcgcaggc gagccgctct acctggatgt gaccgctgcc atcagcgaag gttggcgcac
     1201 catgcatccg cagacctatc cacagatcat cggcgggcgg tatggcctct cgtcgaagga
     1261 attcacgccc gccatggtca aaggcatttt tgatgagatg agcgcgtctg cgcccaggca
     1321 gcatttcacg gtcggcatta acgacgacgt gacgcacagc agcctggctt atgatccggc
     1381 tttctccatc gagccgcagg agaccgtgca gtgcgtcttc tgggggctgg gttccgacgg
     1441 cacggtgggc gccaacaaga actccatcaa gatcatcggc gaggaaacgg acaacgacgc
     1501 gcagggctac ttcgtctacg attccaagaa agcggggacg gtcacgatct cgcacctgcg
     1561 cttcgggccg catcccattc acgcgcccta ccagatcgga gcaggcgagg cgcacttcgt
     1621 ggcctgccac cagttcacat tcctggaacg ctacgatgtg ctcaagtatg cccggcccgg
     1681 cgcggtcttc ctgctcgata gcctctactc accagaagaa gtgtgggaac acctgcccgg
     1741 tgaaacgcag caggctatac gcgacaaacg cctgcggttt tatgtgatca acgcgcacaa
     1801 agtcgccagc gagatcggca tgggagggcg catcaacacc attatgcaga cctgcttctt
     1861 cgccatcagc ggcatcctac cacgcgagga ggccatcgag cagatcaaaa agagcatcaa
     1921 gaagacctat ggcaaacgcg gcgaggccgt ggtgcagcgc aactaccagg ccgtcgatgc
     1981 caccctgcaa catctccacg aggcgctcat ccctgccgaa ac
//
LOCUS       DASZAK010000960         3941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_699848, whole genome shotgun sequence.
ACCESSION   DASZAK010000960 DASZAK010000000
VERSION     DASZAK010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3941
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699848"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="VGT44_16800"
     CDS             <1..997
                     /locus_tag="VGT44_16800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEV2582519.1"
                     /translation="NINFLRCSRLKNAEREHDPSSLGLRFHASVPIYAGDTPIGVLNV
                     ASKDWRELAAEELQLLHIIGDQIGLAIQRARISAEHTQAATRLATIEERNRLAREIHD
                     TLAQGMAAITLQLETADALAPSHPERAQEAIRRALGLARKNLEEARRSVIDLRAASLQ
                     DRTLPEALAALTRDTAQEGITIDYRYEPVSDFPILSPRLEAGVYRIAQEALSNAYKHA
                     KAQQISLTLVVENGELYLYVQDDGRGFVLDEVTQATNSLTGSKVEHFGLTGMSERVKL
                     LGGTVCISSEPGAGTCVVARLPLEAPTRLVNDSGASTFAQQKSSKSQGEEGRGKR"
     gene            994..1683
                     /locus_tag="VGT44_16805"
     CDS             994..1683
                     /locus_tag="VGT44_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2582520.1"
                     /translation="MIHILLADDHPIVREGLRAVLETQPDFEVIGSPEQAASGDEALR
                     LALELQPDILLLDLEMPVMDGVETIRRLRQHPRYGEGFRPRIIVFTAFDNDERIIAAL
                     EAGANGYLLKGAPREDIFNAIRVTMQGGSLLQPVIASKLLRHVGLHSTSRSLAGQHNT
                     LYETLTERELEVLTLLAQGMPNKEIAAHLTISERTAKFHVSSIMGKLGATNRTEAVSL
                     AAQKGLITLHS"
     gene            1969..3021
                     /locus_tag="VGT44_16810"
     CDS             1969..3021
                     /locus_tag="VGT44_16810"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV2582521.1"
                     /translation="MRAFVMKSLGQVGFMEKPIPKPGPDDAIIKTTKALICTSDSHTV
                     HGAIGPRENLTLGHEAVGIVHEVGSNVKLFKPGDRVLVGAITPDWFDPASQAGHSSQS
                     GAPLGGWKFSNSKDGVFAEYFHVNDADGNMAHIPDSVADEVAVYCADMLSTGFMGAEN
                     GQVPLGGTVAIFAQGPVGLMATVGARLRGAGMIIAVESVPKRQELARFYGADMIVDFT
                     KEDVVKRILELTNGLGVDTAIEAIGLDSTFQQCIEVTKAGGFISNIGYHGQGDYVLIP
                     RAAWGVGMAEKTITTGLCPGGSLRMQRLLQLLEMKRVDPTRMTTHTFPFAELDKAFEI
                     MDKKLDGVLKPLIVFE"
     gene            complement(3068..3712)
                     /locus_tag="VGT44_16815"
     CDS             complement(3068..3712)
                     /locus_tag="VGT44_16815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582522.1"
                     /translation="MYLKPDALAEKGFVVLRDYDGKPVPRAEWESLEYMDWKSGGDTN
                     FAPIASALGEMECAGFWDHGKPDKDGIWTKNAEICPTLVQWTKNVGANYGRVRIIKLN
                     PNTYEEAVHNLHLDDNNRLNPDGEGWVVRAWLELSDDPNSYMILREDKDDPSTESRIS
                     MPRNRQLVIDSQRLWHAAWHPGPEPRFALIVSFESGEHLDRWIQSQMPQTSEVR"
BASE COUNT          919 a         1020 c         1080 g          922 t
ORIGIN      
        1 taacatcaac ttcttgcgct gtagccgttt gaagaatgcc gagcgcgaac atgatccatc
       61 ttcccttgga ttgcgttttc atgccagtgt gcccatctat gcgggagata cgcccatcgg
      121 agtattgaat gtggccagca aagactggcg cgagctcgcg gcagaagaac tgcaattgct
      181 gcatatcatc ggcgaccaga ttggcctcgc cattcagcgc gcccgcatct cagccgagca
      241 tacccaggca gcgacacgac tggcaactat cgaagaacgt aaccgcctgg cgcgcgagat
      301 tcacgatacg ctcgcacagg gtatggcggc aattaccctt caacttgaaa ccgccgatgc
      361 cctcgcgccc tctcatccag aacgcgctca ggaggccatt cgccgcgcac tcggccttgc
      421 tcgcaaaaat ctcgaggaag ctcgccgttc cgtgatcgat ttgcgcgccg cttctttaca
      481 agatcgcaca ctccccgaag ccttagcggc cctcacgcga gacacggcgc aggaaggcat
      541 cacgattgac tatcgctatg agccggtcag cgattttccg atcctatctc caagattgga
      601 agccggggtg tatcgcatag cacaggaggc tctttccaac gcgtataaac atgccaaggc
      661 gcaacaaatt tcgctgaccc tcgtcgtcga gaacggcgag ctctacctct acgtgcaaga
      721 tgatgggcgc ggtttcgttc tcgacgaagt gactcaagcg acgaattcat taacaggttc
      781 aaaagtggaa cattttggct taacaggtat gagtgagcgg gttaaactgc ttggtggcac
      841 tgtctgcatt tcgagcgaac ccggtgcggg tacctgcgtc gtcgcccgtc tgccattaga
      901 ggcacctacc cgacttgtca acgactccgg tgcgtcgact tttgcgcagc aaaagtcgag
      961 caagtcgcaa ggggaagaag gacggggaaa acgatgatcc acatcttact ggccgacgat
     1021 catcccatcg tgcgcgaggg actgcgagct gtactggaaa ctcaaccgga tttcgaggtg
     1081 attggttctc cagagcaagc cgcgagcggt gacgaagctc tgcgcctggc attggagtta
     1141 cagcccgata tcctcttgct cgacctcgaa atgcctgtca tggacggcgt tgaaaccatt
     1201 cgccgcttgc gccaacaccc gcgttatgga gagggattcc gcccgcgcat tatcgtcttc
     1261 accgcgtttg acaacgatga acgcatcatc gccgcactgg aagccggagc caatggctac
     1321 ttgctcaagg gcgctcctcg cgaagacatc ttcaacgcta ttcgtgtcac gatgcagggg
     1381 gggtcgctgc tccaacccgt catcgcctct aaattgttga ggcacgtggg tctgcatagt
     1441 acatcacgct ccctggctgg gcagcacaac acgctctatg aaacgctcac tgaacgcgaa
     1501 ctcgaagtat tgaccctgct ggcccagggc atgcccaaca aagagatagc cgcgcatttg
     1561 acgatcagcg agcgcaccgc gaaattccac gtcagctcaa ttatgggcaa gctaggagcg
     1621 accaaccgta ccgaagccgt ctccttagct gcccaaaaag gcttgattac gctgcattct
     1681 tagaaacgtc tttcttcgag atcggctttc tgtccctctt tccccggcag tttttgggaa
     1741 tttggagagc tttgctcttt gccgggatca attctgctcc ccccgtttca tttctgttcg
     1801 atagcgctga atatcgcgaa tagaaataag taattgcaag aaagtgtgat gtttttgtga
     1861 agtagtgcaa ttgaaatgcg gtttccttca agttatcctt gtaaatggat gtgaacgcgt
     1921 gttattcaga gttgattagc aggacactaa agaaggagaa acatgaccat gagggcattt
     1981 gtgatgaaat ccctgggaca ggtgggcttt atggagaagc cgattccgaa gcctggcccg
     2041 gatgatgcca tcatcaagac gacaaaagca ttgatctgta cctcggattc tcacacggtg
     2101 catggggcga ttggaccaag ggagaatctg acactggggc atgaagcggt agggattgtg
     2161 catgaagtgg gaagcaatgt caagctattt aagcctgggg accgggtcct ggtgggagct
     2221 attacaccgg actggtttga tcctgcctcg caagcggggc attcgtcgca gtcgggagcc
     2281 ccgttaggag ggtggaaatt ctccaattcg aaagacggcg tattcgcaga gtactttcat
     2341 gtgaatgatg cggatgggaa tatggcacat attcctgata gcgtggccga cgaagtagct
     2401 gtgtactgcg cggatatgtt gtccaccggt ttcatggggg ctgaaaatgg acaggtgcca
     2461 ttaggcggaa cggtggcgat tttcgcgcag gggccggtag gtctgatggc gacggtagga
     2521 gcgcgcctgc ggggagcggg aatgatcatt gcggtggagt cggtgccgaa gaggcaggaa
     2581 ctggcgcggt tctatggcgc agatatgatc gtcgatttca cgaaggaaga tgtggtcaaa
     2641 aggattctag agttgaccaa tggcctgggc gtagacactg caatcgaggc cattgggctg
     2701 gatagcacat tccagcagtg cattgaggtg acaaaggcag gagggtttat ctccaatatc
     2761 ggctatcacg ggcaaggcga ttatgtactt attccgcgcg cggcctgggg ggtcggtatg
     2821 gctgaaaaaa ccatcaccac tggtctttgt ccaggaggta gcctgcgcat gcagcgactc
     2881 ctgcagttgc tcgagatgaa gcgtgttgat cccacgcgca tgactactca cacatttcct
     2941 ttcgcagaac tcgataaagc ctttgagatc atggacaaga aactggatgg cgtgttgaag
     3001 ccgttgattg tgttcgaata gcgtacattg gggcgaccac caggatcgcc ccaacagctt
     3061 tcacctttta acgaacctca gatgtttggg gcatctggct ttgaatccag cgatccaggt
     3121 gctcgccgct ctcgaaagaa actatcagag caaagcgagg ctcgggtccc ggatgccatg
     3181 ccgcgtgcca taagcgctgc gagtcgatca caagctggcg gttacgcggc atcgaaatgc
     3241 gcgattctgt tgaaggatca tctttgtcct cgcgtaaaat catgtacgag tttggatcat
     3301 ctgaaagttc aagccacgcg cgcactaccc agccttcgcc atccgggttg agacggttgt
     3361 tgtcgtcaag atgcaggtta tgcacggctt cctcataagt attgggattg agcttgataa
     3421 tgcgtacccg cccgtagttg gctcctacat ttttggtcca ttgcaccagc gtaggacaga
     3481 tctcagcgtt ttttgtccag ataccatcct tgtcgggctt accgtggtcc cagaatcccg
     3541 cgcactccat ttcaccaaga gcagaagcga taggcgcaaa gttcgtgtca ccaccacttt
     3601 tccagtccat atactcgagg ctctcccatt ccgccctggg aacaggcttg ccatcgtaat
     3661 cgcgcagtac aacaaagccc ttttcggcaa gggcgtcggg cttaagatac attcagttac
     3721 tccttatcca gtgaacgacg agaaatagat accctggttt gtctcaaaat aaacgctatc
     3781 caggtatcag tttgaatatc cattgaagcc tttgcagagg cttcttttgg taattggcac
     3841 attgcgttct cacattatat gctcaataaa cctttcgcca ttttaccata ggttcgctat
     3901 cgggtgttat gcagtagcca attgtgatga ggttgtgaca t
//
LOCUS       DASZAK010000961         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_416289, whole genome shotgun sequence.
ACCESSION   DASZAK010000961 DASZAK010000000
VERSION     DASZAK010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416289"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VGT44_16820"
     CDS             <1..1129
                     /locus_tag="VGT44_16820"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEV2582523.1"
                     /translation="REEQAMKPPSLTIGIEEEYQIVDPQTRELRSYITQILEEGRLVL
                     REQVKPELHQSIVEVGSSVCQTPAELREELIRLRRAIMELAGKNGLRIAAAGTHPFSS
                     WMTQDITPMERYAGVKQDMQELAQRLLIFGTHVHIGIEDREFLIDAMNVVRYLLPHML
                     CLTSSSPFWMGRNTGLKSYRSIVFRNFPRSGIPRTFGSWSEFQYFVDTLVATNTIPDG
                     SKIWWDVRPNWSYPTVEFRICDVCTRVEEAVCIAAIFQAIILKLWKLRRDNMTFRVYP
                     FDLIEENKWRAVRYGLDGKLIDFGKQQELPARTLIREMIEWFIADVVDELGSRQEIEY
                     AYRIMDEGSSADRQLATFARTGDLNDVVDQLIAETAEGVLP"
     gene            1186..>1579
                     /locus_tag="VGT44_16825"
     CDS             1186..>1579
                     /locus_tag="VGT44_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582524.1"
                     /translation="MSPDGLRVAYVNTTIDAGKHAYRSSIWVIPAEGGEARCFTSTVA
                     NAHSPAWSPDGRWLAFVSDREGEPKDAPREKRASKGKPQLWLMPADGGEARQLTWMPH
                     GASAPTWSPDSRRILFSATVAPLDEETED"
BASE COUNT          319 a          493 c          470 g          297 t
ORIGIN      
        1 gagggaggag caggccatga aaccgccatc tttgaccatc ggcatcgaag aagaatacca
       61 gatcgttgat ccgcagacgc gcgaactgcg ctcgtacatc acgcagattc tcgaagaagg
      121 acgcctggtg ctgcgcgagc aggtcaagcc ggagctgcac cagtctatcg ttgaggtcgg
      181 ctcaagcgtc tgccagacgc ccgccgagtt gcgcgaggag ttgattcgtc tgcgccgcgc
      241 catcatggaa ctggccggaa aaaatggcct gcgcatcgcc gccgccggaa cacatccttt
      301 ctcctcgtgg atgacgcagg atattacgcc gatggaacgc tacgccggcg tcaagcagga
      361 tatgcaggaa ctggcgcagc gcctgctcat cttcggtacg catgtgcata tcggcatcga
      421 ggaccgcgag tttctgatcg acgccatgaa cgtcgtgcgc tacctgctgc cgcacatgct
      481 ctgcctcacc tccagttcgc ccttctggat ggggcgcaac accggcctca agtcttatcg
      541 cagcattgtc ttccgcaact tcccgcgcag cggcatcccg cgcaccttcg ggtcctggtc
      601 ggagttccag tattttgtgg atacgctggt ggctaccaat accattcccg acggctccaa
      661 aatctggtgg gatgtgcgtc cgaactggtc ctaccccacc gtggaatttc gcatctgcga
      721 cgtttgcacg cgcgtggagg aggccgtgtg tatcgcggcg attttccagg ccatcatcct
      781 caaactctgg aagttgcggc gcgacaatat gaccttccgc gtctacccct tcgacctgat
      841 cgaggagaac aaatggcgcg ccgtgcgcta cggcctggat ggcaagttga tcgacttcgg
      901 taagcagcag gagctgcccg cgcgcacgct gatccgcgag atgatcgaat ggttcatcgc
      961 tgatgttgtt gacgaacttg gcagccgcca ggaaatagag tacgcctatc gcatcatgga
     1021 cgaaggctca agcgccgacc gccagcttgc caccttcgcg cgcacaggcg atttgaacga
     1081 cgtggtcgat caactgattg cggagacagc agaaggagta ttgccctgac tcgtaccatt
     1141 tctatcgaag acctctacca atttcaaatt ctgagcaggc cgcgcatgtc gccggatggg
     1201 ctgcgcgtgg cctacgtcaa tacaactatc gacgcgggca aacatgccta ccgttcgtca
     1261 atctgggtga tcccggcgga agggggcgag gcgcgctgtt tcaccagcac agtcgccaac
     1321 gcgcacagcc cggcctggtc gcccgatgga cgctggctgg ccttcgtctc cgaccgggag
     1381 ggcgagccga aggatgctcc tcgcgaaaaa cgcgccagca agggcaagcc gcagctctgg
     1441 ctgatgcccg ccgacggcgg cgaggcgcgc cagttgacgt ggatgccaca cggcgcatcc
     1501 gcaccgacct ggtcgcccga tagtcggcgc atcctcttca gcgcgacggt cgccccgctc
     1561 gacgaagaga ccgaagacg
//
LOCUS       DASZAK010000962         5402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_556830, whole genome shotgun sequence.
ACCESSION   DASZAK010000962 DASZAK010000000
VERSION     DASZAK010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5402
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556830"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VGT44_16830"
     CDS             complement(<1..639)
                     /locus_tag="VGT44_16830"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2582525.1"
                     /translation="MPVDPQTQAVIDYLETLGIPPMNAQTVADARGVMLAMGAMRGEP
                     EPVGNVEDREVPGPEGQISVRIYTPEGKGPFPVLVFFHGGGWVIGNIESHDAVCRSLT
                     NQAGCITVSVDYRLAPEHKFPAAPEDCYAATQWVAANAVTFDGDPTRIAVGGDSAGGN
                     LAAVVSLMVRDRGVPNLVYQLLIYPATDYYLPGTQSIQENAEGYLLTRDDMIW"
     gene            complement(670..1362)
                     /locus_tag="VGT44_16835"
     CDS             complement(670..1362)
                     /locus_tag="VGT44_16835"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV2582526.1"
                     /translation="MPRLIDLSQEIYQGMFVYPGHLKTVIWEHHTHAETVKNFEGGFS
                     YQSRGLMMSDHGPTHVDALSHLDPRPEAPTIDKMSLDLFYGEATCIDVSHKEPRTYIS
                     ASDIDEVIATSGADVRKGDILLFYTGTFNRLHGTTEYLSQYPGLDETGSMWLVEKGIK
                     TFGVDSPSPDNPASRTYPCHMMCRHYGITHFENLANLDQLVGKRFTFMGFPLRIRNGT
                     GSPVRAVALVSD"
     gene            complement(1382..2161)
                     /locus_tag="VGT44_16840"
     CDS             complement(1382..2161)
                     /locus_tag="VGT44_16840"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2582527.1"
                     /translation="MELQDKIAVITGGGRGIGRAIALAYAREGANLVLASRSQEALEE
                     TQSMVEDLGRKALVVPTDIRKEDSVRNLAEQALGHFGRVDILVNNSGIAGPTAPLWDI
                     TLAEWEDTFAVNVTGVYLCCRAFLPGMIERRSGSIIMIASMTGKRPLYGRTPYAGGKL
                     ALVGIARTLAWEVGSHAIRVNVISPGAVEGERITRVVRNQAAAQGISEEEALRQFTSG
                     SPLGRLVPPGDIAAAAIFLASDRAASITGEDLNVSAGTVMY"
     gene            complement(2177..4585)
                     /locus_tag="VGT44_16845"
     CDS             complement(2177..4585)
                     /locus_tag="VGT44_16845"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV2582528.1"
                     /translation="MTTSTNNYAWIGKDIKRREDPDLLTGRAEYTNDVKLPGMLYAAV
                     LRSPYAHARIISIDTSAAKKLPGVHAVLTGNDATEVIDPVPAFCAEPVVERAIAVDKV
                     RYAGEAVVAVAAESRYIAEDALELVQIDWEPLPPVIDLLAAMESDAPKVHENLPSNVV
                     YNQVFTFGDVDGDFARADHVIKRHLRWPRATAAPMEPNGAVCKYDPASGRMDIWSNTN
                     MLNYVGWLIAITLKLPPHMVNFFPMYTGGSFGSKHVISKVVAIAGALSKVTGRPVKFM
                     EDRIDNLTANDSQGPDRIYDAELAVTKEGKFLSLRVHTIDDYGAYFMFAVTGNTNMMS
                     QISGPYTIGSVETGIRAVLTNKNQQGVFRGAGSDVGNWVLERLVDAASEVLGIDGVEL
                     RRQNLIQPNQFPYKMPTGNVYDSGNYPAVLEMALQHADLAHWREEQARARQQGRYIGI
                     GLATCQQRSTYSATEFWFHNPGPATGLTSTPESVRIGIGPTGGFTVTMFSPFWGNSPE
                     TVVSQIVAEEFGVDPATVSVTYDSTSHGLPGAGPGGSRMTVMLSGAVHGAAAKIKEKM
                     SKIAAHLLEASTEDMEIREGRVWVKGTDRSLSIGDIGFKAYWFKFDLPADMESGLEGS
                     FTYDHPYTTPPRDDRKDLGSFYPIMGHAVHIPVVEVDINTGQVTFLKYLAVHDVGTVV
                     NPRSLQGQIRGGIAQGIGIALYEQVRYSPEGQNLTATMNDYLVPTVAEIPNVDVYHHE
                     TPSPFTAYGVKGGGEGGRMVAPPAVTSAVEDALKPLGIKIDEMPITPEKIVQWVKKAQ
                     SQPT"
     gene            complement(4605..5117)
                     /locus_tag="VGT44_16850"
     CDS             complement(4605..5117)
                     /locus_tag="VGT44_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEV2582529.1"
                     /translation="MQEEDFVTIRLSVNGETHRLVVEPRRLLADVLRDDLGLTGTNLG
                     CEHGACGSCTVLVDGQSVRSCLMFAVQASGHEVMTVEGLARNGAMHPLQQAFWEKQGL
                     QCGFCTPGFLMTAYELLQHNPNPSEEEIRDALSGNICRCTGYQHIIEAVQSAAQSMAS
                     SAAVPSGTGA"
     gene            complement(5117..>5402)
                     /locus_tag="VGT44_16855"
     CDS             complement(5117..>5402)
                     /locus_tag="VGT44_16855"
                     /inference="COORDINATES: protein motif:HMM:NF015415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582530.1"
                     /translation="AAVITLDANGAITDARLGLCSVGPAPVRPQAAEALLRGQRPGNE
                     TWAAAAAAVVSVLDNPLSDIHGSADYRRHLAGVLTKQALAVAAERAEGKR"
BASE COUNT         1148 a         1563 c         1447 g         1244 t
ORIGIN      
        1 ccagatcatg tcatccctgg tcaggagata cccctcggca ttttcctgga tggactgggt
       61 acctggcagg taataatcgg tggccggata gattagtaac tggtacacca gattgggcac
      121 gccccggtct cgtaccatca aagacacaac tgccgccagg ttaccacctg cgctatcgcc
      181 tccaacggca atgcgtgtgg gatcgccgtc gaacgtgacg gcattggctg cgacccattg
      241 cgtagccgcg tagcaatcct ccggcgctgc tggaaatttg tgttccgggg caagacgata
      301 gtcaaccgag acggttatgc agccagcctg atttgtgagc gaacggcaaa cagcatcgtg
      361 gctttcaata ttgccgatga cccaaccacc accgtgaaag aagacgagca ccggaaatgg
      421 acctttccct tcaggtgtat agatgcgtac cgaaatctgc ccctccggtc ccggcacctc
      481 gcgatcttca acattaccaa caggttctgg ctcacctctc atcgcgccca ttgcaagcat
      541 gaccccgcga gcatccgcaa cagtttgagc gttcataggt ggtataccta aggtctccag
      601 gtagtctatc actgcctgag tttgtggatc gactggcata gattcgtgct ccttgttgca
      661 aatctctaat caatcactta ctaacgctac tgcccttacc ggcgatcctg taccattgcg
      721 gatgcgcagc gggaacccca taaaggtgaa acgcttgccc actagctgat ccaggttcgc
      781 caggttctca aaatgtgtaa ttccgtagtg acggcacatc atgtggcatg ggtaggtacg
      841 actggcggga ttatccgggc tgggagagtc gacgccaaag gtcttgatac ccttctcgac
      901 cagccacata cttccagttt catccaggcc tggatactgg ctcaggtatt ccgtcgtgcc
      961 gtgaagccgg ttgaaggtgc ctgtatagaa caacaaaata tcgcctttgc gcacatcagc
     1021 gccgcttgtg gcgatcacct cgtcgatatc ggaagcgctg atataggtgc gtggttcttt
     1081 gtgagagaca tcaatacagg ttgcctcgcc atagaataga tcgagagaca tcttgtctat
     1141 ggtaggcgct tccggccgtg ggtccaggtg gctaagggca tcgacatgcg taggaccatg
     1201 atcgctcatc atgaggcctc tggactggta agagaatccc ccttcgaagt tttttactgt
     1261 ctctgcgtgc gtgtgatgtt cccaaatgac ggtctttaaa tgtccagggt agacaaacat
     1321 cccctggtat atctcctgag agaggtcaat gagtctgggc atcgttgaat ctccattcca
     1381 gtcaatacat tactgtgcct gcggaaacat tcaggtcttc gcctgtgatc gaggctgccc
     1441 tatcggaagc gaggaagata gctgcagcgg caatatctcc aggtggaacg aggcggccaa
     1501 gcggtgaacc gctggtaaat tgccgtagtg cctcctcttc gctgatacct tgcgccgctg
     1561 cctggttgcg aactaccctt gtgatgcgct ctccttcaac cgcaccaggg gagatcacat
     1621 tgactcgtat tgcatgtgac cctacctccc aggcaagcgt tctggcgata cccaccaggg
     1681 ccagttttcc gcccgcgtag ggtgtacggc catacagcgg tcgtttcccg gtcatagagg
     1741 cgatcatgat gatagaccct gagcgccgct caatcatgcc tgggagaaac gctcggcagc
     1801 ataaatagac cccggtcaca ttgacggcga aggtatcctc ccattcggca agcgtgatgt
     1861 cccaaagagg ggcggtagga ccagcaatgc cgctgttatt caccagtata tcgacccgac
     1921 cgaagtgacc aagcgcctgc tccgccaggt tacgaacgct gtcttctttg cgaatgtcag
     1981 ttggaaccac cagcgcctta cgcccaaggt cctcgaccat cgactgcgtc tcttcgagcg
     2041 cctcttgcga acgtgaggcc agcacgaggt tggctccttc tctggcatat gcaagggcga
     2101 tggcgcgtcc gatgccgcgc cctcctccag taatgactgc tatcttgtct tgtagttcca
     2161 taagtaattt cctgctttat gttggttgtg actgcgcttt cttcacccac tgcacgattt
     2221 tctcgggcgt aattggcatt tcgtcgattt tgatgccgag aggtttgagc gcatcctcta
     2281 ccgcgcttgt gaccgccggc ggcgctacca tgcgtccacc ctcgccaccg cccttgacgc
     2341 catacgcggt gaaaggcgag ggtgtctcgt gatggtaaac atcgacattt ggtatctcgg
     2401 ccacagttgg caccaggtag tcgttcatgg tcgccgtcag gttctgacct tcggggctgt
     2461 agcgcacttg ttcgtacagt gctatgccga ttccttgagc aatgcccccg cgaatctggc
     2521 cctgcaatga gcgcggattt accacagttc caacatcatg cacagcaagg tatttgagga
     2581 aggtaacctg gccagtgttg atatcgactt ccacaactgg aatatggacg gcatggccca
     2641 tgatggggta gaaggagccc agatctttgc ggtcatccct gggaggcgtt gtgtagggat
     2701 gatcataggt gaagctcccc tccaggccgc tttccatatc cgccggcagg tcgaatttga
     2761 accagtaggc tttgaagccg atatccccta tgctcagcga acgatccgtg cctttgaccc
     2821 agacacgccc ttcacgaatc tccatatctt cagtgcttgc ctctaataaa tgagcagcga
     2881 ttttggacat cttttccttg attttagctg ccgcgccatg aactgctcct gagagcatca
     2941 ccgtcatgcg gctgcctcct gggccagcgc ctggcaatcc gtggctggtg ctatcatagg
     3001 taacgcttac cgtagccgga tcaactccga attcctcagc aacgatctga gacacgaccg
     3061 tttcaggact attgccccag aatggcgaga acatggtgac cgtgaaccca ccggtggggc
     3121 caataccaat acgcacgctt tcaggcgtgc tcgtcaggcc cgtagcgggg ccaggattat
     3181 ggaaccagaa ctcggtcgca ctgtaggtgc tgcgctgttg gcaggtagcc agtccgatgc
     3241 cgatataacg gccctgctgg cgcgcgcgcg cttgctcctc gcgccagtgt gccaggtcag
     3301 catgctggag tgccatttcc agtacagcag gatagttgcc gctgtcatag acgttgccgg
     3361 tcggcatctt ataggggaac tgattgggtt ggatcaagtt ttgccgtcgc agttctaccc
     3421 cgtcgatacc caatacctca gaagcagcat caaccagtcg ctccagcacc cagttgccga
     3481 catccgaacc ggcgcccctg aatactccct gttgattctt attggttaac actgccctga
     3541 tacccgtctc aacactgccg atggtgtatg ggccgctgat ctgcgacatc atgttggtgt
     3601 tgcctgtaac tgcaaacatg aagtaggcgc cataatcgtc tatcgtgtgc acgcgcaggc
     3661 tcaggaactt gccctcttta gtgacggcca gttcggcatc gtagatgcgg tctgggccct
     3721 gactatcgtt cgccgttaaa ttatcgatac ggtcttccat gaacttaacc gggcgcccgg
     3781 tgaccttcga gagtgcgcca gcaatagcga ccaccttgct tatcacatgt ttgctgccga
     3841 aactgcctcc tgtatacata ggaaagaagt tgaccatatg gggtggaagc ttgagggtaa
     3901 tcgcaatcaa ccaccctaca tagttgagca tattggtgtt cgaccagata tccatgcgac
     3961 cactggccgg gtcgtacttg catactgcac cgtttggctc catgggcgcg gcagtggctc
     4021 tcggccagcg caaatgccgt ttgatgacat gatccgcacg ggcgaaatcg ccatctacat
     4081 caccaaaggt gaacacctgg ttgtagacaa cgtttgacgg aagattctca tgcacttttg
     4141 gcgcatccga ctccatcgcc gccagcagat caatgacagg tggcaatggc tcccagtcaa
     4201 tctgcaccaa ctcaagagca tcctcagcga tgtagcgact ttcggctgcc accgcgacta
     4261 ctgcttcacc ggcataacgc accttgtcga cagcgattgc acgttcaacg accggctcgg
     4321 cgcaaaaggc tggcaccgga tcaattacct cggtcgcatc attaccggtc aggacagcgt
     4381 ggacgccagg cagctttttg gcagcactgg tatcgatact gatgatgcgg gcatgagcat
     4441 agggactgcg cagtacggcg gcgtagagca tgccgggcaa ctttacatcg ttagtgtact
     4501 ccgctcgacc agtgagcaga tcgggatctt cacgccgctt gatgtctttg ccaatccatg
     4561 catagttatt ggttgatgtt gtcatagttt atctcctttg cggctcacgc tccggtgccc
     4621 gatggtacgg cagcgctcga ggccatggac tgggcagcgc tttgcaccgc ctcgatgatg
     4681 tgctggtagc cggtacagcg acagatgttg cctgagagcg cgtcgcgaat ctcttcttca
     4741 ctcggattcg ggttgtgctg taataactcg tatgcggtca tcaggaaacc gggcgtgcaa
     4801 aacccgcact gaagcccctg cttctcccag aaggcctgct gcagaggatg catcgcccca
     4861 ttacgagcta acccctcgac ggtcatcacc tcgtggccgc tggcctgcac tgcgaacatc
     4921 aggcacgagc gcacgctctg tccatctacc agcacagtac aagaaccgca ggctccgtgt
     4981 tcgcagccga ggttcgtccc tgtcaatccc aaatcgtcac gcagcacatc ggcaagcaag
     5041 cgacgcggct caaccaccag gcggtgcgtc tcaccattga cagataagcg gatcgtcaca
     5101 aaatcttctt cttgcattat cgcttccctt ctgctcgctc tgcggcgact gccaatgcct
     5161 gtttagtcaa gacccctgcc aggtggcggc gatagtcagc agagccatgg atatctgaaa
     5221 gtggattgtc cagtacggag acgacagcgg cggcggcggc agcccaggtt tcgttaccgg
     5281 gacgctgccc gcgcagcaag gcttcggctg cctgcgggcg cacaggggcg ggacccacgc
     5341 tacaaagtcc caaacgagca tctgttattg cgccattcgc atccagcgtg atcacggcgg
     5401 ca
//
LOCUS       DASZAK010000963         9338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_25186, whole genome shotgun sequence.
ACCESSION   DASZAK010000963 DASZAK010000000
VERSION     DASZAK010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9338
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25186"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..352)
                     /locus_tag="VGT44_16860"
     CDS             complement(5..352)
                     /locus_tag="VGT44_16860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582531.1"
                     /translation="MFMANRTRPLGVTIIAILVAIVGIFDVIVGILGLVILIGIIPLI
                     LGILALILAAGLWNLRTWAFWATVILAVLNIINDIFDVTRNQGSHFVSIALWVIVLVY
                     MLLDRNVRAAFRT"
     gene            complement(573..899)
                     /locus_tag="VGT44_16865"
     CDS             complement(573..899)
                     /locus_tag="VGT44_16865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582532.1"
                     /translation="MLSRTRPLGVTIIAVLVAIGGIAEIISGLQFLGIIAIVLGILTL
                     VLAWGLWTLQPWAFWSTIVLEAINALENLLNLRYGGSSIIGLIIPVAIIVYMLYDRNV
                     RAAFRT"
     gene            1098..1171
                     /locus_tag="VGT44_16870"
     tRNA            1098..1171
                     /locus_tag="VGT44_16870"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1132..1134,aa:Pro,seq:ggg)
     gene            complement(1247..1507)
                     /locus_tag="VGT44_16875"
     CDS             complement(1247..1507)
                     /locus_tag="VGT44_16875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582533.1"
                     /translation="MIPQIEAHEAIYEQPLREEPGGKVYPPVIDNKNMLRLIWFGVAM
                     VALLAFAAICVVAVGGTAGWISFIAAALTIMVIASTAISTIK"
     gene            1807..2685
                     /locus_tag="VGT44_16880"
     CDS             1807..2685
                     /locus_tag="VGT44_16880"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEV2582534.1"
                     /translation="MELKKKRSLGDIVVGFWLLAHVEPIILILAVVTLFAVRATWPHP
                     PWTTILLLLAGQAAMQFSIGILNDYFDRQRDVLSGKNKPIVMGLIRPQEALVAGLLMI
                     VIMLIILAPLNFLALLAALGYLALGQIYNLGLKTTPLSGILFALAMPLLPLYAFAGVG
                     RIPSMVIWFLPIGAILGVALNLANSLPDIEEDRAGGARSLAVTFGVKGSFIACPLLIA
                     LAAILIGTLTIFQLVPAQLWLMLPILVLTVLGLVSLLIFFGPARSRRTRKIYFYLVAL
                     LCLLLAGGWFIALKLS"
     gene            2682..4367
                     /locus_tag="VGT44_16885"
     CDS             2682..4367
                     /locus_tag="VGT44_16885"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV2582535.1"
                     /translation="MNTAPEPTQPDSAFCPIDESIAVVIVGAGPTGLAAGNLLGMAGV
                     ATLIVERNPGLSDIPKAIALDDEGLRICQAMGLSAAINDCIVSDISVQCVAKGQLLAK
                     VAPLSRRNGYPLISTFNQPEFEAVLLDGLRRFSCVETRFQHTVETFEQGTDCIVVSMR
                     TPTGALQKVRCAYLLACDGGRSTVRHLLNIPMRGMTFEQKWLVIDSISLAASSPVVKI
                     FCDPGRPAVSIPAPHNGRRWEFMLLPGETEQDMLETATISALLQQTDALPQTQIIRQA
                     VYAFHAARAKSFSQGCVFLLGDAAHMMPPFGGQGLNSGLRDAHNLAWKIAMVLQNRAA
                     PQLLSTYDKERSRHAAQMIAFASFLGTLFMSSRRSLAYCRNRLIQMLFALPVTRAYFR
                     EIRIKPRAKYTAGFFLFNWRKKRLVGSLLPQPEVINQHGQRVLLDEVLGTGFALLRRH
                     SNPEEAFASVNSDFWENLGTLFVCIQSDDAPLKRSAKKRTLHYSSYQAPCESQMVRSS
                     PIVVIRSAHKDFPGVSQDYFVVVRPDRFILGIFKEEEADAFVSAFQRLLQQSA"
     gene            complement(4388..5224)
                     /locus_tag="VGT44_16890"
     CDS             complement(4388..5224)
                     /locus_tag="VGT44_16890"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEV2582536.1"
                     /translation="MSLYMLSHRWPVLLRIFAVSFFTLLAARGHVVWSIIALVIGAYA
                     AMQVSIALMNDYCKRERDAQGRLDIANPHSLVSPRIALTGAIAMMALMAVLLAPLPPL
                     TWVISLCYLALGQGYNLKLKATMYGSIVFALVMLLIPVYAFVGVGRIVSFLFWLVPIG
                     FLLGVTLNLANSLPTIEEDTRSGAQTLTVTLGVKRSFMACQLLIVVSATLIGLLAITQ
                     VVPTQPWVLVTTLILACLGIEAMLLFFGPEKPIETRRLYFYVVALTCIALAGGWLLGV
                     IA"
     gene            complement(5356..6594)
                     /locus_tag="VGT44_16895"
     CDS             complement(5356..6594)
                     /locus_tag="VGT44_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEV2582537.1"
                     /translation="MHDKPNILIVDDDAALLQAMPQALSLRLTAIQVDTSDSALEALD
                     LIKEHDYDAIVSDIKMPGMDGLALLAKVQEYRPETPTLLITGHGEHDLAVQALRGGAY
                     DFIQKPIDRDYMVAALSRAIQTCRLRREVAENQRALELHNLSLERIVQNRTRELVDAN
                     AAKDKFLGMVSHELKTPLTTLKGRAQLLLRQTRREMGENIVTAGLEDIERSINRLQVL
                     VDDLLDASLVDTNMFVIHRRRCNLVELCRKLLNEFISDDRPSLHFESPGEAIEAEVDA
                     DRIGQVLLNLLSNARKYSPEGYPITVTLQRVGFEAIISVRDMGEGIPSEALPHIFDQY
                     YRAPGVKVRAGSHNGLGLGLFISQKLVERHAGRIEVQSEPDQGSIFSVHLPIFVDPTV
                     EEIETTNLAPHAQAVWTILH"
     gene            complement(6595..7824)
                     /locus_tag="VGT44_16900"
     CDS             complement(6595..7824)
                     /locus_tag="VGT44_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2582538.1"
                     /translation="MSNETAATIMATRSPDEPAQTALWQANKELQEQMLVLETRVKKS
                     EERRRALMHILSDLNMVNRKLADQRKAMIHILADYEQDRSRLAKQTERLDNSRRALMH
                     ILQDSHRSNLRLENSRKAMIHIMSDLRETTEEVQRREQELREKQEQLVQAGKLATLGE
                     LTTGVAHELNNPLNNIGLFVGNAIDLISLGTMDIEPESILRELHSAMQQVRKATEIIS
                     HLRTFGRAAPVSFEALDIRQVLERAISLMREQLRLRQIDVQLFFPEEAPVVFGNAIQL
                     EQVFINLLTNARDALVNSPTKIITITCQVETELVALRFCDTGPGIPLELEQRIFDPFF
                     TTKEVGAGTGLGLSITYGIIKDHQGAIVVENRCGNHPDNALCVGCAQTECREDPGNGA
                     HFLIHLPRTGQIPVGGV"
     gene            complement(7824..9128)
                     /locus_tag="VGT44_16905"
     CDS             complement(7824..9128)
                     /locus_tag="VGT44_16905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEV2582539.1"
                     /translation="MPDTDISITTLAASASQATKPHGSLDADIIIKNAPDPVFVSDLE
                     GKILSANDAIYELLGFRTDEVLEQSLSRFISPEETREFTAALREVIERGATRNARLNP
                     RSASDEIISTSLNASALRDSDGRVIGAIGILRDMRELDKAQAYADSLIKNAPDPVFVS
                     DLEGKILSANDAVYELLGFRTDEVLEQSLSRFISPEETREFTAALREVIERGVIRNTR
                     LNPRSASGEIIPTSLNASALRDADGKVIGAIGILRDMRELDKALAYSDSLIKNAPDPV
                     FVSDLEGKILQANDAVYELLGFRPDEVLEQSLSRFISPEETREFLAALREVIERGATR
                     NARLNPRSASGEIIPTTLNASALRDADGRVIGAIGILRDMREYEKVVRDLQESKSELQ
                     EKIQDLEKFEEVVVGRELKMISLEKEVENLKRELEKVHRGLS"
BASE COUNT         2078 a         2532 c         2496 g         2232 t
ORIGIN      
        1 tacatcacgt gcggaaggcc gcgcgcacat tcctatcgag gagcatatac accagaacga
       61 tcacccatag cgcaatactg acgaagtggg aaccctggtt acgtgtgacg tcaaaaatat
      121 cgttgatgat atttaagacc gccagtatga cggttgccca gaatgcccag gtcctgagat
      181 tccacagtcc tgctgccagg attaatgcga gtatgcccag gatcagtggg ataatgccga
      241 tgaggatgac caatcctaat ataccgacga ttacgtcgaa gatgcccaca atagcaacca
      301 ggatcgcgat aatggtgacg cctagtggcc gcgtgcgatt cgccataaac atgagaagaa
      361 cctccttttt gtacagaaag tcgttgctac ttagcttctc ttttgttaag agagctttca
      421 atgaagacgc cacgactgta aagcttatca cttctataca cgcggaacag cctggaagcg
      481 tcgtagcgtg acgttgcgat cgcgacatga aaagggagaa cgatgcgtat agtcgttctc
      541 ccttttcatg tcgcgagaag tttcgcagga tatcacgtgc gaaaagccgc gcgcacattg
      601 cggtcgtaga gcatatagac gatgatcgcc actggaatga tcaggccaat gatagaactg
      661 ccgccatatc gtagattcag aagattttca agcgcattga tagcctcaag aacaatcgtt
      721 gaccagaatg cccagggttg gagggtccag aggccccagg caagcaccag tgtgaggatg
      781 cccaggacta tagcaatgat gcctaaaaac tgtaagccgg agattatttc ggctattcca
      841 ccaatagcta ccagaacagc gataatggtg acaccaagtg gccgggttcg tgagagcata
      901 attgaacctc cattcataac aagaaacaaa ctcaaaagct gagctcttac aaagagtgta
      961 acctatatac gagagtagct atcagatagt tatatttcga cagtatccgt catgcaaaca
     1021 ttaacgtctc gcgaattgcc tgccaaaaat cttgcgtatt tcctctattt ctgctatact
     1081 ggctatgttc acaagagcgg ggcgtagcgc agcccggtag cgcaccacaa tggggttgtg
     1141 gtggtcggag gttcaaatcc tctcgccccg accgcacacg cgaggtagga ataatgctga
     1201 tggagcgatt tcccacctcg ctctatttgt cctctcgtta gctctcttat ttaatggtcg
     1261 agatggctgt ggaagcgatg accatgatgg tcaaagcagc agcgatgaaa ctaatccatc
     1321 ctgctgtccc tccgacggcc accacacaga tcgctgcaaa ggcgagcagc gccaccatcg
     1381 ctaccccgaa ccagatgaga cggagcatat tcttgttgtc aatcactggc gggtacactt
     1441 tccctcctgg ctcttctctc aatggttgct cgtatattgc ctcatgggct tctatttgtg
     1501 gtatcatgtt ctcttcctcc tctctggagt tgatgagtgt ctgtgggaag aatctcttct
     1561 tctaagaagg aagataccac atcggcatca cgtccttatc acatgagcag cctgcacctt
     1621 cacaatgctc tcacatctgg aaagcacgca accattccca tgctggtttc ttggttctgg
     1681 acgaaataga agtagccttg aacgttggta aacaccaaat ctttatcatg ccagttgcct
     1741 acctcaaggg acatggtata gaattattga cgaggcttat cgtataggat cagggagttt
     1801 tagcgcgtgg aactaaagaa gaaacggagt ctgggtgata tcgttgttgg cttctggttg
     1861 ctcgcccatg tcgaacctat tatactcata ctggcggtgg tcacgctctt tgcggtgcgc
     1921 gccacatggc cacatcctcc ctggacaacc attcttctgc tccttgcagg gcaggctgcc
     1981 atgcaatttt ctattggcat actcaatgac tattttgacc gtcaaagaga tgtacttagt
     2041 gggaaaaaca aacccatcgt catgggattg atccgtccgc aagaggctct cgtggcgggt
     2101 ctgctgatga tcgtcatcat gctgataatt ttagcgccgc tcaatttcct ggccttgctc
     2161 gccgcgctcg gttatctcgc gctggggcag atatacaacc tgggactgaa aacaacccct
     2221 ttgagcggca tcctttttgc cctggctatg ccattgctcc ccttgtatgc ctttgccgga
     2281 gtagggcgta ttccgtctat ggtcatttgg tttctgccaa tcggcgctat actcggtgtg
     2341 gcgctgaatc tcgccaactc cttgccggat attgaagagg acagggcagg cggagcgcgt
     2401 tcacttgctg tgacgttcgg cgtgaaaggc tcgttcattg cctgtcctct gctcatcgct
     2461 ctggcagcta ttctgatcgg taccctgacg atcttccaac ttgttcccgc tcaactctgg
     2521 ctgatgctgc ccatccttgt tctcacggtc ctgggtttag tgtcactgct tatcttcttc
     2581 ggaccggcgc ggtcgagacg cacacgcaaa atctactttt acctggttgc gctgctctgc
     2641 cttctgttgg caggcgggtg gtttatcgca ctcaaactct catgaataca gcgccagagc
     2701 cgactcaacc tgactccgca ttctgtccga tcgatgaatc cattgcggtc gtgattgtag
     2761 gggctggacc gactggactg gctgccggaa atttattggg aatggcgggt gttgctacgc
     2821 ttatcgttga gcgcaatccc ggattaagcg acattcccaa agccattgcg ctcgacgatg
     2881 agggcctgcg catctgccag gctatgggcc tcagcgccgc tatcaacgac tgcatcgtgt
     2941 cggatatctc ggttcaatgt gtggcaaagg gacagctcct ggcaaaagtg gcgcctttga
     3001 gcaggagaaa tggctacccg ctcatctcaa catttaatca acctgaattt gaagcagtgc
     3061 tgctggatgg actccggcgt ttttcctgcg tcgagacgcg ctttcagcat acagttgaga
     3121 cgtttgagca gggcacagat tgcatcgtcg tttcgatgcg cacaccaaca ggcgcgctcc
     3181 agaaggtgcg atgcgcctat ttactcgctt gcgacggcgg gagaagcaca gtgcgccact
     3241 tgctcaacat tcccatgcgg ggcatgacat ttgagcaaaa atggctggta atcgactcca
     3301 tttccctggc agcttcctct cctgtcgtca aaattttctg cgatccagga cgcccggcag
     3361 tatccatccc ggctccgcat aacggcaggc gctgggaatt tatgctcctc cccggcgaaa
     3421 ctgaacagga tatgctggaa acggcgacta tttccgcgct acttcaacag accgatgcgc
     3481 tcccacagac gcagatcatt cgccaggctg tgtatgcatt ccatgcggca cgagcaaaat
     3541 ccttctcaca aggatgcgtc ttccttttgg gggatgccgc ccacatgatg ccccccttcg
     3601 gcggccaggg attgaacagc gggctgcgtg acgcccataa ccttgcctgg aaaatagcga
     3661 tggtgctaca gaatcgcgcc gcgccgcaac ttctctcaac ctatgacaaa gagcgttcca
     3721 ggcatgccgc tcaaatgata gccttcgcgt cttttttagg cacgctgttc atgtcctcaa
     3781 gacgctccct ggcttattgt cgcaatcgcc tgatacaaat gctctttgct ttgcctgtca
     3841 cacgcgccta ttttagagaa atacgcataa agcctcgagc gaaatataca gccggtttct
     3901 ttctcttcaa ctggcggaag aaacgcctgg tgggatcgct attgcctcag ccagaagtaa
     3961 tcaaccaaca tggccagcgt gtactccttg acgaggtgtt ggggacaggt tttgccttgc
     4021 tgcgccgcca ttccaatcca gaagaagcgt ttgcaagcgt caattccgac ttttgggaga
     4081 acctggggac gctcttcgtg tgtatccaga gcgatgacgc accgctaaaa agaagcgcaa
     4141 agaagcggac ccttcattac agttcctatc aagcgccatg tgagtcgcaa atggtccggt
     4201 cgtctccaat tgttgttatc cgttccgcgc ataaagattt tccaggggtg agtcaggatt
     4261 acttcgtcgt cgtccgcccc gatcgcttta tcctgggcat tttcaaagag gaagaggccg
     4321 atgcgtttgt atcagctttc caacgcctgc tccagcaaag cgcatgattc ggctgagaac
     4381 tggccagcta tgcgatcaca cctagcagcc agcccccggc cagagcaatg caggtgagcg
     4441 ctacgacgta gaaatataac cttcgtgttt caattggttt ttccgggccg aaaaacagca
     4501 gcatggcctc gattcccagg caggccagga tcagcgtcgt gacaagcacc cagggctgcg
     4561 ttggaaccac ttgagtgatg gcaagcaggc cgatcagcgt tgcgctcacc acgatgagca
     4621 actggcaggc catgaacgac cgcttcaccc ccagtgtgac ggttagcgtc tgcgcgccgc
     4681 ttcgcgtgtc ttcctcaatg gtgggaagcg agttcgccag gttgagcgtc acgccgagca
     4741 aaaagccaat cggcaccagc cagaacagga acgagacaat gcgcccgacc cccacgaagg
     4801 catacactgg aatgagtagc ataaccagcg cgaagacgat gctgccatac attgttgctt
     4861 ttagttttag attgtagccc tggcccagcg caaggtagca gagagagatg acccaggtca
     4921 gaggcggtag gggtgcgagc aagacggcca taagcgccat catagcaatt gcgccggtga
     4981 gggcgatgcg gggcgagacg aggctgtgtg ggttggctat gtccagcctg ccctgcgcat
     5041 ctcgctcacg tttgcagtag tcattcatca atgcgatgga gacctgcata gcggcgtatg
     5101 ccccgatcac caacgcgatg atgctccaga caacatggcc ccgggcggct aacaacgtaa
     5161 agaacgagac ggcgaagatg cgcaggagaa ccggccaacg atggctcagc atatacaggc
     5221 tcataagccg ttcgttaagt tgacgtttat gtgtgatttc cataagtgtg cttgtccatt
     5281 ttcgataaag taggttttcc tactatgaac gaaacatggc acacaagtgg gggaatcgta
     5341 ttcaaatgtt cgctttcaat gcaagattgt ccagaccgcc tgtgcatgtg gagctaaatt
     5401 cgtcgtctct atttcctcca cggtgggatc gacgaaaatc ggcaggtgta cggaaaagat
     5461 gctgccctgg tcaggctcgc tctgcacttc gatgcgcccg gcatggcgct ccaccagttt
     5521 ttgcgagata aacagaccca ggcccaaccc gttgtgcgag cctgctcgca ctttgacacc
     5581 gggggcacga tagtactggt caaagatatg cggcagcgct tcggacggga tgccctcgcc
     5641 catatctcgt accgatatga tcgcctcaaa tccaacacgc tgcaaggtga cggtgatagg
     5701 atagccctca ggcgagtatt tgcgcgcgtt tgaaagcaag ttgagcagaa cctggccaat
     5761 gcggtccgca tctacttctg cttcaatggc ttcgccaggt gactcaaaat gaagcgatgg
     5821 ccgatcgtcg gaaataaatt cgttcagtaa cttccggcag agttcaacaa gattgcacct
     5881 tctccggtgg attacgaaca tattggtgtc cacaagcgag gcatctagca ggtcgtcaac
     5941 gagtacttgc aagcgattga tggaacgttc tatgtcttcg aggccagcag taactatgtt
     6001 ttcgcccatc tctcgcctgg tctgccgtag aagcaactgt gcccttccct tcaatgttgt
     6061 caatggtgtc ttgagttcgt gtgagaccat gcccagaaat ttatctttcg cggcgttggc
     6121 atccacaagc tcgcgtgtac gattttgtac aatgcgttca agtgagaggt tatgcagttc
     6181 aagcgcgcgc tggttctcgg ccacctcgcg gcgcagccgg catgtttgta tggcgcggct
     6241 cagcgcggcg accatataat cgcgatcaat cggcttttgg ataaagtcat acgccccgcc
     6301 tcgcagcgcc tgcaccgcca ggtcatgctc gccgtgaccg gtaatcagca gcgttggcgt
     6361 ctctggccgg tactcctgta ccttcgccag cagcgccagt ccatccatgc cgggcatttt
     6421 gatgtcactc acgatcgcat cgtaatcatg ctccttaatc aggtcgagcg cttccagggc
     6481 tgagtctgag gtatccacct ggatggcggt caagcggagc gacaacgctt gaggcatagc
     6541 ctgcaagagc gctgcgtcat catcgacaat gaggatattt ggtttgtcat gcatctacac
     6601 acctccaact ggtatctgac ctgttcgcgg aagatggatc aagaaatgag cgccgttgcc
     6661 agggtcttcc ctgcattcag tctgagcgca gccgacgcat aatgcgttgt caggatggtt
     6721 gccacacctg ttttcgacga cgatcgcgcc ctggtgatcc ttgataatgc cgtaggtaat
     6781 cgatagaccc agacccgttc ccgctccgac ttctttggtg gtgaagaaag gatcaaagat
     6841 gcgttgttcc agttccaaag ggataccggg accggtatca cagaaacgga gcgctaccag
     6901 ctccgtctct acctggcagg tgatggtaat tatcttggtt ggggaattga cgagcgcgtc
     6961 ccgcgcgttt gtcaacagat tgatgaagac ctgctcgagc tgaatagcat tcccaaacac
     7021 gaccggggct tcctctggaa aaaacagttg cacatcaatc tggcgtaaac gcaactgctc
     7081 gcgcatcagc gagatagcgc gttcgaggac ctgccggatg tccagcgcct cgaagctgac
     7141 cggggcggcg cgcccgaagg tgcgcaggtg cgaaataatc tctgtcgctt tacgcacctg
     7201 ctgcatggcg ctgtgcagtt cgcgtaggat gctttcgggc tcgatatcca tcgtccccag
     7261 cgagatgaga tcaatggcat tgcccacaaa cagaccaata ttgttaagcg gattattcaa
     7321 ctcgtgcgcg accccggtcg tcagttcgcc cagcgtcgcc agcttgcccg cctggacgag
     7381 ctgctcctgc ttttcgcgca attcttgctc gcggcgctgt acctcctccg tcgtttcgcg
     7441 caggtcgctc atgatgtgaa tcatggcctt acggctgttc tccaggcgaa gattggagcg
     7501 gtgcgaatct tgcaaaatgt gcatgagcgc gcggcgagag ttatcgagtc gttcagtttg
     7561 cttcgcaagt cggctgcgat cctgttcgta gtccgccagg atatgaatca tggctttgcg
     7621 ttgatccgcc agcttgcgat tgaccatatt cagatcgctc aagatatgca tgagggcgcg
     7681 gcggcgttcc tcgcttttct ttacgcgcgt ttcgagtaca agcatctgct cttgcagctc
     7741 tttgtttgcc tgccacagcg ccgtttgtgc tggttcgtcc ggcgagcgcg ttgccatgat
     7801 ggttgctgcg gtctcgttgc tcattaactg agacctcgat gcactttctc tagctcgcgc
     7861 ttgaggttct ctacctcttt ttccagagag atcatcttga gttctcgccc gaccacgact
     7921 tcttcgaatt tctccaggtc ttgaatcttt tcctgcaact ctgactttga ctcctgtaaa
     7981 tcgcgtacca ctttctcgta ttcgcgcata tcacgcagga taccaatagc gccgatcact
     8041 ctcccatcgg catcgcgcag ggcagaggca ttcagggtag ttgggatgat ctcgccggaa
     8101 gcgcttcgcg ggttgaggcg cgcattacgg gttgcgccgc gctcgatcac ctcgcgcagg
     8161 gccgccagaa attcgcgcgt ctcttccggg ctgatgaagc gcgaaagcga ttgctccaat
     8221 acttcgtctg ggcggaaccc cagcaattcg tacacagcgt cgttggcctg gagtatcttg
     8281 ccttccaggt cggagacgaa caccgggtcg ggcgcattct tgatgaggct atccgagtag
     8341 gccagcgcct tgtcgagttc gcgcatatca cgcaggatac caatagcgcc gatcactttt
     8401 ccgtcggcgt cgcgcagggc agaggcgttc aagctggttg gaatgatttc gccggaagcg
     8461 ctgcgtgggt tgagacgtgt attgcgaatc acgccgcgtt cgatcacctc gcgcagggcg
     8521 gcggtaaact cgcgcgtctc ttccgggctg atgaagcgcg aaagcgattg ctccaacacc
     8581 tcgtcggtac ggaaccccag cagttcgtac acagcgtcgt tcgccgaaag aatcttgcct
     8641 tccaggtcgg agacaaacac cgggtcgggc gcattcttaa tcagactatc ggcataggct
     8701 tgcgccttgt cgagttcgcg catatcacgc aggatgccga ttgcgccgat gactctgcca
     8761 tcggaatcgc gcagggcgga agcattcaag ctggtcgaga tgatctcatc ggaggcgctg
     8821 cgcgggttga ggcgcgcgtt gcgggttgcg ccgcgctcga tcacctcgcg cagggcggca
     8881 gtaaactcgc gcgtctcctc cgggctgatg aagcgcgaaa gcgattgctc caacacctcg
     8941 tcggtgcgga accccagcag ttcgtaaata gcatcgttcg ccgaaagaat cttgccttcc
     9001 aggtcggaga caaataccgg gtcgggcgca tttttgatga tgatatcagc gtccagcgac
     9061 ccatgtggtt tggtggcctg ggatgcagag gcggctaagg tggttatcga aatgtcggtg
     9121 tcgggcatag aaaatacctc ctgcttctca gaagcgccat gataacttct acacgtttga
     9181 taatttcagt gtagcaaatc gcgccttttt gccacaaacc aatgagaaga tagtcgtgat
     9241 gagttaatat gggcacgttg caattggagg aaaagtgaca ggttttaccg gactggcagg
     9301 cgatccatca tggccttgaa ccagcgtgtg cgaatctc
//
LOCUS       DASZAK010000964         1921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_556897, whole genome shotgun sequence.
ACCESSION   DASZAK010000964 DASZAK010000000
VERSION     DASZAK010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556897"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1142
                     /locus_tag="VGT44_16910"
     CDS             <1..1142
                     /locus_tag="VGT44_16910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2582540.1"
                     /translation="YSDIRQGVRDVCRAFDSAYWQGVEEASGYPEAFVEALTKAGWLA
                     ALIPEEYGGGGVSLTEASVILEEINRSGANSGACHAQMYTMGALLRHGSLEQKQRYLP
                     RIASGELRLQSFAVTEPTTGTDTTRTKTFAEKRGERYLVNGQKVWISRVQHSDVMLLL
                     ARTTPMGQASKKTEGLSLFLVDVRKALGNGMTVRPIRNMVNHETNEVFFDNVEVDTEN
                     LVGEEGKGFSYILDGMNAERILIAAECIGDSYWFIDRARIYAGERIVFDRPIGQNQGV
                     QFPIAQAYAKTRAADLMRYEAARLFDAQQPCGAEANMAKLLAADASWQAANVCLQTHG
                     GYGFAAEYDIERKFRETRLYQVAPISTNLILSYLGEHVLGMPRSF"
     gene            1142..>1921
                     /locus_tag="VGT44_16915"
     CDS             1142..>1921
                     /locus_tag="VGT44_16915"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV2582541.1"
                     /translation="MSGWTKGRLVMSERIGFIGLGLMGKPMARHLLAAGFQLTVFNRS
                     RAAIDELVRDGARAASSPRGVAEQSDIVITMLPDGPDVEAAVEGRDGLLDGAREGMLV
                     IDMSTISPLTTRRLASVLEARGMHMLDAPVSGGEEGAKAASLSIMVGGGEADFRRAQP
                     VFTALGKTITYCGACGSGQVVKACNQVLVALIIEAISEALVLGAKSGVAPDIILKVLS
                     GGLAQTRFMDLRGASMIQHRFEPGGKAAFHLKDLRIVKQLAQ"
BASE COUNT          376 a          545 c          661 g          339 t
ORIGIN      
        1 agtactccga tattcgccag ggggtgcgcg acgtgtgcag ggcctttgat agcgcctact
       61 ggcagggcgt tgaggaagca tcgggctatc ctgaagcgtt cgtggaggcg ctgacgaagg
      121 ctggctggct ggcggcgctg atcccggagg agtacggcgg aggaggagtc agcctgacag
      181 aggcgtcggt gatcctggaa gagatcaacc ggtcgggggc gaattctggg gcgtgtcacg
      241 cgcagatgta tacgatgggc gcgctgctgc ggcacggttc gctggagcag aaacagcgct
      301 acctgccgcg catcgcgtcg ggcgaattgc ggctgcagtc gtttgccgtg acggaaccga
      361 ccaccggcac cgacacgacc aggacaaaaa ccttcgcgga gaagcgcggg gagcgctatc
      421 tcgtgaatgg gcagaaggtt tggatttcgc gcgtgcagca ttcggacgtg atgctgctgc
      481 tggcgcgcac gacgccgatg gggcaggcca gcaagaaaac ggagggactg tcacttttcc
      541 tggtggatgt gcgcaaagcc ctgggaaacg gcatgacggt gcgccctatt cgcaatatgg
      601 tcaaccacga gacgaacgag gtctttttcg ataacgtcga ggtagacacc gagaacctgg
      661 tcggggaaga gggcaaggga ttctcgtaca tcctggatgg catgaacgct gaacgcatcc
      721 tgatcgccgc ggagtgcatc ggcgatagct actggttcat cgaccgggcg cgcatctatg
      781 ccggcgaacg catcgtcttc gaccgcccca tcggccagaa ccagggcgtg cagttcccga
      841 tagcgcaggc gtatgccaag acgcgcgcgg ctgacctgat gcgctacgag gcggcgcgcc
      901 tcttcgatgc gcaacagccc tgcggcgcgg aggcgaacat ggcgaagctg ctggccgccg
      961 acgcatcgtg gcaggcggct aatgtctgcc tgcaaacgca cggcggctat ggcttcgcgg
     1021 ctgagtacga tatcgagcgc aaatttcgcg agacgcggct ctaccaggtc gcgccgatct
     1081 cgaccaacct gatcctgtcc tacctgggcg aacacgtgct gggtatgccg aggtcgttct
     1141 aatttcagga tggacgaaag gaaggttagt aatgagcgaa cgcatcggct tcatcggcct
     1201 cggtctgatg ggcaagccca tggctcgtca cttgctggcg gcaggtttcc aactgaccgt
     1261 cttcaatcgc agccgggcgg ccatcgacga gcttgttcgc gatggcgcga gggcggccag
     1321 ctcgccacgc ggggtcgctg agcaaagcga catcgtcatt accatgctgc ccgacggacc
     1381 cgatgtcgaa gcggccgtcg aagggcgcga tggcctgctc gatggggcgc gcgagggaat
     1441 gctggtgatc gatatgagca ccatctcgcc gctcaccacg cgccggctgg ccagcgttct
     1501 tgaagcgcgc ggcatgcata tgctcgacgc gccggtcagc ggaggcgaag aaggggcaaa
     1561 agcggcgtcg ctctccatca tggttggagg cggcgaagcg gatttcaggc gagcgcagcc
     1621 ggtctttaca gcgctgggga aaacgatcac gtactgcggt gcctgcggtt ccggccaggt
     1681 cgtgaaagcc tgcaaccagg tactcgtcgc gctcattatc gaggccatct cggaagcgct
     1741 agtgctgggc gcgaagtcag gcgtcgcgcc cgacatcatt ctgaaagtgc tgagcggcgg
     1801 gctggcgcag acgcgcttca tggatttgcg cggcgcatcg atgattcagc atcgcttcga
     1861 gccgggaggc aaggccgctt ttcacctgaa ggatctcagg atcgtcaaac agctcgcgca
     1921 a
//
LOCUS       DASZAK010000965         3119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_665063, whole genome shotgun sequence.
ACCESSION   DASZAK010000965 DASZAK010000000
VERSION     DASZAK010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665063"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..725
                     /locus_tag="VGT44_16920"
     CDS             297..725
                     /locus_tag="VGT44_16920"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV2582542.1"
                     /translation="MHEDTLARAALFNSLDKKELQALASECQERSFDTGATIIKQDTS
                     GTGLYIITSGHGKIVKEHGPDRAEEELGTFGPGSVIGEMALLDDLPRSANVVATEPVV
                     ALVLPVWAFRGTVRNHPEIAIKLLSVLSKRLRKLESKDDQ"
     gene            complement(869..1324)
                     /gene="nrdR"
                     /locus_tag="VGT44_16925"
     CDS             complement(869..1324)
                     /gene="nrdR"
                     /locus_tag="VGT44_16925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEV2582543.1"
                     /translation="MKCPYCGGTESRVKDSRDVGDAIHRRRECEQCKGRFSTYERVEQ
                     SQLMVIKRDQRRELFDRQKLVAGISKACEKRPMPVEEIEKAVEEIEQEVYRLGKQEIP
                     SSVIGELVMDRLRSMDKIAYIRFASVYRSFGDVETMFAEIQNLLNREKE"
     gene            complement(1511..1723)
                     /locus_tag="VGT44_16930"
     CDS             complement(1511..1723)
                     /locus_tag="VGT44_16930"
                     /inference="COORDINATES: protein motif:HMM:NF042840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HEV2582544.1"
                     /translation="MSQLPIDQHITYQLQYRKCGKPTCSTCRDGQGHGPYWYAYWREG
                     SRLRSGYVGKAPPDLKTLATPVLATT"
     gene            2176..2811
                     /gene="raiA"
                     /locus_tag="VGT44_16935"
     CDS             2176..2811
                     /gene="raiA"
                     /locus_tag="VGT44_16935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV2582545.1"
                     /translation="MQLIIKGKQMDVPTRLRQHIERKVQKLSRLVGSDARVEITISEE
                     QTRSAKDRYSVHLALTNNASPIRSEVSAVNAMTALDIVLDKVATQLGRQKGRQAARRH
                     HTPPLKILALSRAGELTAVEAQQAEEGHEQLEMPSIDEEQNEDIWSKVLEIRRISTRP
                     MDDQEVIAHMDRHKLSYYPFFNVETQSVNVMYRLDQGGYGLLVPALEEVTE"
     gene            complement(2801..>3119)
                     /locus_tag="VGT44_16940"
     CDS             complement(2801..>3119)
                     /locus_tag="VGT44_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582546.1"
                     /translation="EFYHQRFELHYLWIRPPLTTGFAFVPRLIFLLRDSTGVEWAGDR
                     GGLLFERADLSNGPDTVVYQGSARFRPLPSDDARKLLVRAADPLGQFETPSLQPWEFE
                     ITL"
BASE COUNT          728 a          823 c          843 g          725 t
ORIGIN      
        1 ggccgatcaa tcggcggtga gcgcggtaaa ccggctccta cgggttgcat tatatcccat
       61 acatctctat cagaaaacgt gtttcagggt atgttcatct gtctttggct ctattgtagt
      121 gcgttggcag gataaagtca aaataactca gctggccctg gctcgactgc ttgacacgtt
      181 gccacttgac acacgtctct cgttgagata ctctaaactc acatcatagc gcatcaggac
      241 acatcgctct cgttcttcca tcaccgaaag caatttggca caaaggagct aatagtatgc
      301 acgaagacac tcttgcgcgc gcagcacttt tcaacagcct cgataagaaa gaattacagg
      361 cgctcgccag cgaatgccag gagcgctcgt ttgacacggg cgcgaccatt atcaagcagg
      421 atacgagtgg gaccgggttg tatatcatca ccagcgggca tggtaaaatc gtgaaggaac
      481 atggccccga ccgagccgag gaagagcttg gcacctttgg acccggcagc gtgatcggcg
      541 aaatggcgct gctggacgat ctgccccgtt cggccaatgt ggtcgcgacc gaacccgttg
      601 ttgcacttgt gctgccggtc tgggcgtttc gcggtacggt gcgcaatcat cctgaaatag
      661 ctatcaagct gctttcggtt ttgagcaagc gccttcgcaa actcgaaagt aaggacgacc
      721 aataaggcga tgtgaacgaa cccgttgtag gggagcaatt cattgcgccc tggtttgctt
      781 cacatatctg cctggatcga gggcgcaatg aattgctccc ctacaacggg ttcattagac
      841 aagcacatcg cttcaccttc ggtcacgtct attctttttc tctgttcaag agattctgta
      901 tttctgcgaa catcgtttct acatcgccga atgaacggta gacgctggcg aagcggatgt
      961 aggcgatttt gtccatgctg cgcaggcggt ccatcaccag ttcgccaatt acgctgctgg
     1021 ggatttcctg tttgcccagg cgatagactt cttgctcaat ctcctcgact gctttttcaa
     1081 tttcctcaac gggcatcgga cgcttctcac aggctttgct gatgccggcc accagtttct
     1141 gacgatcgaa cagctcgcgt ctctggtcgc gtttgatgac catgagttgc gattgctcga
     1201 cccgttcata tgtgctaaag cgccctttgc actgctcgca ttcgcggcgg cggtgaatag
     1261 cgtcgcccac atcgcgagaa tccttcacac gcgactcagt ccctccacaa tatggacact
     1321 tcatcctgcg cctcccaaag atggttgcgg gccatcgtcc ctacggacga tgggcgcctt
     1381 gtttcgtcaa tgtgcattat cggcgctgac tacgataaac cgactccttc agggtgatat
     1441 atatcaactc tgtctaagga atgcagctat tgtatcatag gtgtgaaggg acaaagagga
     1501 tgaaggatgg tcaggttgtt gccagcacag gagttgcgag ggtcttcaaa tctggtggcg
     1561 ccttaccgac atagccagag cgcaggcgcg atccctcgcg ccagtaagca taccagtagg
     1621 ggccgtgtcc ctgcccatca cggcaggtgc tgcaggttgg tttcccgcat ttgcgatatt
     1681 gcagttgata ggtgatatgc tggtcgatgg gtagctgtga catgtgccgc tttccctccc
     1741 actaacaaat agtaccatca gaacgaaatt ttctcaatag agaataccac caaattatga
     1801 acattgtcta aatggatgtt acgaacacat gaagaagtgt tcatacaaat gtctcaccgt
     1861 atgtcaaatc gttgtgtcac ataacagctt tgtcatgtgt ttctgtaaag gttgtagcgg
     1921 gctgtcaccc tgagcgcagc gaagggtctc ggcctgccgg gcggtgagat ccttcgctgc
     1981 gctcaggatg acaggcagga cacctcgctc aggatgacag ccaggacatt ctcaagtctg
     2041 ctgcattgtc gttctgctct actatccgcc gggaagtgct ataatagagg tagaccattc
     2101 ctgttgtcct ggcgcattgg gtttcctatt caggttgctt gtatgttttg ttggattgga
     2161 aagagaaggt aagccatgca acttattatc aaagggaaac agatggatgt ccccacacgt
     2221 ttacgtcagc acatcgaacg caaggttcag aaactttccc gcctggtagg atcggacgcg
     2281 cgtgtagaga ttacaatctc ggaagaacaa acgcgcagtg cgaaagatcg atactcggtt
     2341 cacctggcgc tgaccaacaa cgcctcccct attcgcagcg aggttagcgc ggtgaatgcc
     2401 atgactgcac tggatatagt gttggataaa gtagccacgc aactgggtcg tcagaagggc
     2461 cgccaggccg cgcgccgcca tcatacgccg cccctcaaga tactcgctct ctcgcgcgcc
     2521 ggagaactga ctgcagttga ggcgcagcag gcggaggaag gtcacgaaca gcttgagatg
     2581 cccagtatcg atgaagagca gaacgaggat atctggtcaa aagtgctgga gatcaggcgc
     2641 atttccactc gccccatgga cgatcaagaa gtcatcgccc atatggacag gcataagctg
     2701 tcctattatc cgttcttcaa cgtagagact cagagcgtca atgtgatgta tcgtctggac
     2761 cagggcggat atggtttact tgtaccagcc ctggaggaag tcacagagta atctcgaatt
     2821 cccagggctg tagggatggt gtctcaaatt gacctagcgg atcggcggcg cgcacaagca
     2881 acttgcgcgc gtcgtccgag ggaagcgggc gaaaacgcgc cgatccctgg tagaccaccg
     2941 tgtccgggcc attgctcaaa tccgcgcgct cgaacagcag cccgccgcgg tctccggccc
     3001 actcgacgcc ggtagagtct cgcagcagaa agatgaggcg aggcacaaag gcaaatccgg
     3061 ttgtgagcgg tgggcgaatc cacagatagt gcagctcgaa acgctggtgg tagaattcc
//
LOCUS       DASZAK010000966         2085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_665096, whole genome shotgun sequence.
ACCESSION   DASZAK010000966 DASZAK010000000
VERSION     DASZAK010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665096"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VGT44_16945"
     CDS             complement(<1..987)
                     /locus_tag="VGT44_16945"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEV2582547.1"
                     /translation="MLTIITFLPLVGVLALYLVPRPTAWRWIAVMTSALVLGVGIALA
                     ILFNWSNGTLQFAVRVDWIPSLGSSYHLAVDGLSLPLIVLTALLTFLALLYAWKQEKF
                     SREYFALYLVMETGLIGFFSTMDLLLFYVFFEIALVPMYFIIGIWGDEKRVEAALKFF
                     LYTRVGSLAVLLSILALYLGTNPHTFDLPTIIAAHPYAGAGIGASLVLLGFLVGFGIK
                     LPVVPLHSWLPDAHTEAPTEGSVILAGLLLKLGGYGLLRVALPTVPGAFSAWAVPIAA
                     LAVASAVYGAAVAMAQTDLKRLIAFTSINHMGYVLLGVAVAAVATASASDRAA"
     gene            complement(1003..>2085)
                     /locus_tag="VGT44_16950"
     CDS             complement(1003..>2085)
                     /locus_tag="VGT44_16950"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEV2582548.1"
                     /translation="LQVVAWIGVLTSLLGGAAALVESDLKRTLAYSTMSQLGLMFVGL
                     GSGSLLAGVLLLLAQALYKATLFLAAGAVDHAVDGTAFARVGGLAKRMPLTAIAFALS
                     AAALAGLPVTLAVPPKDPVLAAAWQANTALFIAALLASLLTALYSVRAFGLVFLGAPS
                     GPAQEAHEAQPGLLVPLLTMAALIPLGLLADAALLGYPLSRLLGESIPDVPLVTALAL
                     ALAVAGVAVGLWSRQVWPQFVVWSVLEPVTKLFTGELGFKTLYRAFTQLGFWIIGWLG
                     TFDRVVFDTLTRSLVRGVLFVVRACAGIDRRALDAALGDLGAGILAFSQRVRALQTGR
                     IENYLLAVLVWGLGVIAIAVLAAIVR"
BASE COUNT          524 a          644 c          571 g          346 t
ORIGIN      
        1 cgccgcgcgg tcagacgcgc tcgccgtagc taccgctgcg actgcgacac ccagcagcac
       61 gtatcccatg tgattgatgc tggtaaaggc aatcagccgt ttcagatctg tctgcgccat
      121 cgccaccgcc gcgccataaa ctgccgaggc cacggccagg gcagcaatcg gcaccgccca
      181 ggcgctgaac gcaccgggca cagtcggcag ggctacccgc agcaagccgt aaccgcccag
      241 tttcagcagc agtcctgcca gaatcacact gccctctgtt ggagcctcgg tgtgcgcatc
      301 gggtagccag gaatgcaggg gcacaaccgg cagcttgatg ccgaaaccga ccaggaaacc
      361 cagcagcacc agcgaagctc caattccggc ccccgcgtat gggtgggcag caatgatggt
      421 cggtagatcg aaggtatgcg gattcgttcc caggtagagc gccagaattg agagcaggac
      481 cgccaggctt cccactcgtg tatagaggaa gaacttcaat gcagcctcga cacgcttctc
      541 atccccccat atgccgataa tgaagtacat gggaaccagg gcgatctcga agaaaacata
      601 gaacaacagc agatccattg tgctgaagaa accgatcaaa cctgtctcca ttacaagata
      661 aagcgcgaag tactcgcggg agaatttctc ctgcttccaa gcatagagca aggccaggaa
      721 ggtcagcagc gccgtcagta caatgagcgg gagcgacaac ccatcaacgg cgagatgata
      781 actggagccc agcgaaggaa tccaatcgac acgtaccgcg aattggagcg tcccattgct
      841 ccagttgaag aggatagcca gtgcaattcc tactcctaac accagtgcac tcgtcatcac
      901 ggctatccag cgccacgccg tcggtcgcgg aacgaggtag agcgcaagca ctcccaccag
      961 cggaagaaaa gtgatgatcg tcagcattac atccatactc ccttatcgca caatggcggc
     1021 aagaaccgct attgctatca cacccagacc ccacaccaag accgcgagca agtaattttc
     1081 gatacgtccc gtttgcaaag ccctcactct ttggctgaag gcaaggatac ccgcgcccag
     1141 gtctcccagg gccgcatcca acgctcgtcg atcaatacca gcacaggctc gtacgacaaa
     1201 cagaactccc cgcacaagcg agcgggtaag cgtatcgaaa acgacgcgat caaatgtgcc
     1261 taaccagccg attatccaga aaccaagctg tgtgaaggcg cggtacaacg ttttgaaacc
     1321 caattcgccc gtaaacagct tcgtcacggg ttcaagcacc gaccagacca caaactgcgg
     1381 ccagacctgt cgcgaccata aacctactgc aactcctgcg acagcaagag cgagtgccag
     1441 cgccgttacc aggggaacat ctggaatact ctctccaagc agtcttgaga gtggatagcc
     1501 gagcagagca gcatctgcca gtaatcctaa agggataagc gcggccatcg tcagcaaggg
     1561 cacgagcagt cctggctgcg cttcgtgggc ttcctgtgca ggtcccgagg gtgcaccgag
     1621 aaaaaccagg ccaaaagctc gcacgctgta taacgcggtc agcaaacttg ccaggagtgc
     1681 cgctatgaaa agtgcagtat tcgcttgcca ggcagccgcc agtacggggt cttttggtgg
     1741 taccgccagt gtcacgggca gtccggcgag cgcggctgcg ctgagagcaa aagctatcgc
     1801 ggtgagcggc atacgctttg cgagtccacc cacgcgtgcg aaggcagtgc catcaacggc
     1861 gtgatcgact gctccggccg ccaggaacag cgttgccttg tacagggcct gcgccagcag
     1921 cagcagcaca cctgctagca ggctgccgct gccgagaccg acgaacatca gtccgagctg
     1981 gctcatggtt gagtaggcta gcgtgcgctt caagtcgctc tctaccagtg ctgccgctcc
     2041 gcccaggagt gatgtgagga cgcctatcca ggctacgact tgcaa
//
LOCUS       DASZAK010000967         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_274409, whole genome shotgun sequence.
ACCESSION   DASZAK010000967 DASZAK010000000
VERSION     DASZAK010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274409"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VGT44_16955"
     CDS             <1..391
                     /locus_tag="VGT44_16955"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2582549.1"
                     /translation="IVGISEIVLWAADKERSLRFYRDLLGLEVISPPTLPNVFLKVGE
                     GRAGIPQMVVLVPKTEEVKARPSGYQLHHMAFELPEADFDAQHAALVAAGYAPRGGKH
                     PVLASRTMYIDDPDGNEVEFICRAPAL"
     gene            421..2220
                     /locus_tag="VGT44_16960"
     CDS             421..2220
                     /locus_tag="VGT44_16960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEV2582550.1"
                     /translation="MAAMLTLPHWDMSVVFPNMESQEFQEGFSRAVRDIEELAQLFDR
                     YAIAKQDALPVDDNAVLAFETVLQRYNAVLEESRTLNAYITCFVTTNTLDTLAQAKMS
                     EFQQSNMILSQLATRFAAWIGSLDVEALIERSALARSHAFMLRKAKIRAAHLMPPAEE
                     NLASELNLSGGSAWEKLHSDVTSQIAVTLELDGEYKELPMSVVRSLAFETDRETRRRA
                     YEAELEGWKRAAVPLAAAMNGIKGEVNAVCRRRGWESPLAASLFDNNIDRQTLDAMMT
                     AARESFPDFRRYLRAKARALGVEQLAWYDLFAPIGKEGRTWTFEEAESFIVEQFGAYS
                     SRLSDFAARAFREHWIDAEPRRGKVDGAYCTWLRKDESRVFANYKAAYGGVSTLAHEL
                     GHGYHNLNLAQRTVLQRSTPMALAETASIFCETIIRHAALGKASRQEQLLILEDSLQG
                     SCQVVVDITSRFIFEQAVFERRRPRELSIDELNGLMLNAQRETYGDGLDENALHPYMW
                     AMKPHYYDSTISFYNYPYMFGLLFGLGLYAQYQQEPAAFRQSYDALLSSTGLADAATL
                     AGQFGIDIRSVAFWRASLDIVRQDIDTFEGLVG"
     gene            complement(2301..>3110)
                     /locus_tag="VGT44_16965"
     CDS             complement(2301..>3110)
                     /locus_tag="VGT44_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582551.1"
                     /translation="VRLLLGPIQDSTAPRALKYFDIFTEPYLREDHELHEEYMQLMKR
                     LTEVEGFTEFLALARRLPIHINPAALLNLYAASEAPFYTCRVLLPWRYEPDIRLQFKS
                     MVNLIFPDAMVVRPDIRSYQVTLVPENDETDVALMLLPHLAGQRPQWRDEPIARQFLW
                     LLAIVKMVEEGRKSGEDDTMWNPQDTYKQVLQGLALRYDTTTEDEIKTIIAEIQATLE
                     VMDRNGDMDKGKLLALLRSYTITEPALQALSDFLTQEIEAEAQIQIRRRDR"
BASE COUNT          626 a          881 c          950 g          653 t
ORIGIN      
        1 aattgtcggt atctctgaaa tagtcttgtg ggcagccgat aaagagcgat cattgcggtt
       61 ttatcgcgat ctgcttggcc ttgaagtgat atcgccgcca acgctgccca acgtctttct
      121 caaggttggc gagggaagag caggcattcc gcagatggtt gtgcttgtgc caaaaacgga
      181 ggaagtgaag gcgcgtccat cgggctacca gcttcaccac atggccttcg agctgccgga
      241 agccgatttc gacgcgcagc acgcggccct ggtggcggca ggctacgcgc cacgtggggg
      301 caaacatccc gtcctcgcca gccgcacaat gtatatcgat gacccggacg gcaacgaggt
      361 ggaattcatc tgccgcgccc cggcacttta atctatcagg aaaggatttg ttaccgtaga
      421 atggcagcta tgcttacact tccgcattgg gatatgtctg tcgtctttcc caatatggaa
      481 tcacaagaat ttcaagaagg tttctcgcgc gccgtgcggg atattgaaga actggcgcag
      541 ctctttgatc gctacgccat cgccaagcag gacgcgcttc ccgtcgatga taacgcggtg
      601 ctggcttttg agacggtctt gcagcgttac aatgccgtgc tggaagagag ccggacgctg
      661 aatgcctata tcacctgctt tgtaacgacc aatacgcttg acacgctggc tcaggcgaaa
      721 atgagcgagt tccagcaatc caacatgatc ctatcgcagt tagccactcg tttcgcggcc
      781 tggatcggct cgctggatgt agaagcgctg atcgagcggt cggcgctggc gcggtcacac
      841 gcctttatgc tgcgcaaagc gaagatacgc gcggcccacc tgatgccccc ggcagaggag
      901 aacctggcct cggaattgaa cctgagtggc ggctccgcgt gggaaaagct gcacagcgat
      961 gtcacctcgc agattgccgt gacattggaa ctggatggcg agtataaaga attaccgatg
     1021 agcgtggtgc gcagcctggc ctttgagacc gaccgcgaga cgcgccgccg cgcctacgaa
     1081 gctgaactgg agggctggaa gcgggctgcc gtgccgctgg ccgccgcgat gaacggcatc
     1141 aagggcgagg tgaacgccgt ctgccgcagg cgcggctggg agtcgccact cgcggcatcg
     1201 ctgttcgata acaatatcga ccgtcaaacg ctggatgcca tgatgacggc ggcgcgcgag
     1261 tccttccccg actttcgccg ctacctgcgc gccaaagctc gcgcgctggg tgtggagcaa
     1321 ctggcctggt acgatctttt tgctcccatc ggtaaagaag ggcgaacctg gacgttcgag
     1381 gaggcagagt cgttcatcgt cgagcagttc ggcgcgtact cgtcacggct ctcggacttc
     1441 gcggcgcggg cctttcgcga acattggatc gacgccgaac cgcgccgggg caaagtggat
     1501 ggcgcgtact gcacgtggct gcgcaaagac gagtcgcgtg tcttcgccaa ctataaagcg
     1561 gcctacggcg gcgtcagcac gctggcgcac gagctgggac acggctatca caacctgaac
     1621 ctggcgcagc gcaccgtctt gcagcgttca acgccgatgg cgctggcgga gacggccagc
     1681 atcttctgcg agacgatcat ccgccatgcc gccctgggca aggccagccg gcaggagcaa
     1741 ctgcttattc tggaggactc gctgcagggt tcctgtcagg tggtggtcga tatcaccagc
     1801 cgcttcatct tcgagcaggc tgtctttgag cggcgcaggc cacgcgaact ctccatcgac
     1861 gaactgaacg ggctgatgct gaacgcgcag cgcgagacct acggcgacgg tctggatgag
     1921 aacgcattgc acccgtatat gtgggcgatg aagccgcact attacgacag cacgatctcg
     1981 ttctacaact acccctacat gtttggcctg cttttcgggc tgggtctcta cgcgcaatac
     2041 cagcaggagc cggcggcgtt ccggcagagc tatgatgcgc tgctctccag caccggcctg
     2101 gccgatgccg cgacgctggc cgggcaattc ggcatcgata tccgctcggt agcgttctgg
     2161 cgcgccagcc tggatatcgt gcggcaggat atcgatacgt tcgaggggct ggtgggatga
     2221 tgttgcagta cctggtaatg ccttagtagg aacacacaaa tcccgtagga gcacggtaaa
     2281 ccggctccta cgtatggagt tcatctgtcc ctacgcctga tctgaatttg agcttctgcc
     2341 tcgatctctt gcgtgagaaa atcgcttagc gcttgcagtg ctggctccgt gatggtatag
     2401 gagcgcagca gagcgagcag cttacctttg tccatatcgc cgttgcgatc catcacttcg
     2461 agggtggcct ggatctcggc tatgatcgtc tttatttcgt cttcggtggt ggtatcatag
     2521 cgcagtgcaa gcccttgtag gacttgtttg taggtatcct gtgggttcca catcgtatcg
     2581 tcctctccac tcttgcggcc ttcttctacc atcttgacga tggccagcag ccagagaaat
     2641 tgccgggcga tcggctcatc tcgccattgc gggcgctgcc ctgccaggtg gggcagcagc
     2701 atcaacgcta cgtctgtttc atcgttctcc gggacaagcg ttacctggta gctgcggata
     2761 tctggccgga ccaccatggc gtcggggaag atgagattca ccatcgattt aaattgcagg
     2821 cgaatatccg gttcgtagcg ccagggcaac aacacacggc aggtgtagaa gggcgcttcg
     2881 gaggcggcgt acaggttcag cagcgcggca ggattgatat ggatgggcaa gcgccgggcg
     2941 agcgcaagaa attcggtaaa gccctctacc tcggtaagcc gcttcatgag ctgcatatac
     3001 tcctcgtgca gttcgtgatc ctcgcgcaag tatggctcgg tgaagatatc gaagtatttg
     3061 agggcgcgcg gagcagtgct atcctgaatc ggccctaaca gcagccggac
//
LOCUS       DASZAK010000968         1798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_593712, whole genome shotgun sequence.
ACCESSION   DASZAK010000968 DASZAK010000000
VERSION     DASZAK010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593712"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VGT44_16970"
     CDS             <1..1015
                     /locus_tag="VGT44_16970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582552.1"
                     /translation="PTWRAHALQEGMEQMLVDGLVAYWVYQGWGNIGASSGDEYELLA
                     IMQQAAQSGEDALESLRTRIRRAVYQEATLQWHYEIQTMPPVFVADVRADRPARLAGI
                     GSMDDAPRIMSREQMAQLCTWMRDHDASTAVLVSSVPAILPPLIGFAEYIAGVRPFQA
                     RPAGLRHRFGSMLAGAQQKLMQRMSFDHWPVFGATWRELVELLDARRNDLVILSGDVH
                     FSYNMSARRSARKRRAVLYQLVSTPFKNTLEQRDKRLVVGQAWLKQAIYGGLRSRVLP
                     LMWAKGGRRVPSDMLFQNVVALVTFWPQARNEGGYDIRQVYLGVQGETLVEVAVCDVH
                     YSE"
     gene            1040..>1798
                     /locus_tag="VGT44_16975"
     CDS             1040..>1798
                     /locus_tag="VGT44_16975"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2582553.1"
                     /translation="MTELFDIVIAGAGHNSLITAAYLARAGLSCLVLEARPEIGGDTS
                     TEELTLPGFLHDTCSTTHSIFLDSPIWRDQELPLAEYGLEYIQADPASHVVFPDGTSI
                     TQWVDIDRTCEEIARFSKRDADTYRKMIGEWRTVAPLFGKIRYAPAGWSPPLSELLAQ
                     HPQGGIWLRRQALSAWDIIDATFEDWHVKSWMIWFAEGTLQPAERPGTGTLAYSFVSG
                     RQRNGWAIPKGGSRSLPQALRRIIEAHGGAILTNK"
BASE COUNT          339 a          504 c          584 g          371 t
ORIGIN      
        1 acccacatgg cgggcgcacg cgctgcaaga ggggatggag cagatgctgg ttgatgggct
       61 ggtggcctac tgggtctacc agggatgggg taatatcggc gcgagcagtg gggacgagta
      121 cgagttgctc gctatcatgc agcaggctgc tcagagcggc gaggatgcgc tggagagctt
      181 gcggacgcgc attcgccggg cggtctacca ggaggcgact ctccaatggc actacgaaat
      241 ccagacgatg ccgccggtct tcgttgccga tgtgcgcgcc gaccgccccg ctcgcttagc
      301 gggtataggt tctatggacg atgctccgcg tatcatgagc cgggagcaga tggcgcaact
      361 gtgcacctgg atgcgcgatc acgacgcatc gaccgccgta ctggtttcat cggttccggc
      421 catcttgcca ccactgatcg gcttcgcgga atatatagcg ggtgtgcggc catttcaagc
      481 aagacctgcc ggtctgcggc atcgattcgg gtcaatgctg gcaggcgcgc agcaaaaact
      541 gatgcaacgt atgagctttg atcactggcc ggtcttcgga gcaacatggc gtgagcttgt
      601 cgaattgctc gatgcgcgca ggaacgatct ggtgatcctg tcgggcgatg tccacttctc
      661 ttataacatg agcgcacgtc gatctgccag gaagagacgt gctgtgttgt accagctcgt
      721 ctcaaccccc ttcaagaata cgctggagca acgcgacaag cggctggtgg ttgggcaagc
      781 ctggctcaaa caggcaatct acggcggcct gcgctctcgt gtgctgcctc tgatgtgggc
      841 gaagggcggg aggcgtgtcc cctctgatat gctattccag aatgtcgtcg cgctagtgac
      901 gttctggcct caggcgcgca atgagggcgg gtatgatatc cggcaagtct acctgggagt
      961 gcagggcgag acgctggtgg aggtcgccgt ctgtgatgta cattatagtg aatagtagtc
     1021 cctattccag gaggaaccga tgaccgaatt gttcgatatc gtgattgccg gagcgggcca
     1081 caatagtttg attaccgccg cgtacctggc aagagcaggg ctgtcctgcc ttgtgctgga
     1141 ggcgcgcccg gaaatcggag gcgatacgtc tactgaggag ttgacgctgc ccggcttcct
     1201 gcatgatacc tgctcgacga cgcactcgat cttcctggat agccccatct ggcgcgacca
     1261 ggagctgccg ctagccgagt atggattgga gtatatccag gcggacccgg cttcccacgt
     1321 ggttttcccg gatggtacgt ccatcacgca gtgggtcgat atcgatcgca cttgcgagga
     1381 gatcgctcgc ttctcgaagc gcgacgcgga tacctatcgc aaaatgattg gcgagtggcg
     1441 aacggtggct cctcttttcg gcaagatacg ctatgccccg gcgggttgga gtccgcccct
     1501 cagcgaattg ctcgcgcagc acccgcaggg cggcatctgg ctacgtcggc aggcgctttc
     1561 ggcctgggac atcatcgacg cgaccttcga ggattggcac gtgaagtcct ggatgatctg
     1621 gtttgccgaa ggaacgttgc aacctgccga acgtcccggt accgggacgc tggcgtactc
     1681 gttcgtgtcc ggtcgccagc gcaatggctg ggcaattccg aaaggcggat cccgcagcct
     1741 gccccaggcg ctgcgccgta tcatcgaggc gcacggcggc gcgattctga ccaataag
//
LOCUS       DASZAK010000969         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_130477, whole genome shotgun sequence.
ACCESSION   DASZAK010000969 DASZAK010000000
VERSION     DASZAK010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130477"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VGT44_16980"
     CDS             complement(<1..193)
                     /locus_tag="VGT44_16980"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEV2582554.1"
                     /translation="MSKITVLGAGNIGGTLGRKWATAGHQVAFGVSDPNGERAQKLRG
                     ELGDTVSIGSVAEALSTSPD"
     gene            complement(329..1300)
                     /locus_tag="VGT44_16985"
     CDS             complement(329..1300)
                     /locus_tag="VGT44_16985"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV2582555.1"
                     /translation="MDQPLSLTPLSEGPEYTTPDTMRREEFRAMGTAISLLLPAVQAD
                     EGLRIVRELFAEWEQTLSRFLPVSELSRLNARPGESLPVSDLLYHVLATALTAAQATQ
                     GMYDPAMLDQLVQIGYDRTFDELPAERPDPLIPGEPGGGWRGIRVDTIRRRVMLPPGV
                     KLDFGGIAKGMAVDAALASLLERGITPALVNAGGDLAVDGVPPGDGQWMLSVPGRNRF
                     WSIPVIRGAVATSGIARRHWKQGNTIRHHLLDPRTGLSAESDLWSVTVVTDRCEQAEI
                     SAKVAFILGSRDGAAFLRKHHIAGLLVREDGSWQSVPPWPTELMNEL"
     gene            complement(1301..>2265)
                     /locus_tag="VGT44_16990"
     CDS             complement(1301..>2265)
                     /locus_tag="VGT44_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2582556.1"
                     /translation="LTMAVFFSVATFYHQYYLTEMAPAIAALFGIGIVTMWQDYRRSS
                     WHGWLLPLALFATALEQIHILNSYPAWGQWLIPAIAILCTVGALTLVVLRLSWRFDFP
                     FDRLRYMLPALGIGVLALMIAPAAWAGIPVLQDVSVQLPVAGPTQQGGFGGIAGGNIG
                     VTSSANTDTALITYLEANQGSATYLVATASSQTADQLILESNKPIMALGGFSGSDPIL
                     TTSSLAALVKNGTVRFFLLNSIGGGFQPPTQFSENPPEGFFDRPDRRFGDGGFGGGGF
                     GGRGQSSLTSWVTQNCASVPSSKWQSATSTGGFGRGQLYDCAGK"
BASE COUNT          454 a          671 c          702 g          438 t
ORIGIN      
        1 catctggact ggtggagaga gcttcggcta ccgatccgat tgatactgtg tctcccaact
       61 cgccacggag tttctgcgct ctctctccat tggggtcact tactccaaat gccacctggt
      121 gtccggctgt ggcccatttg cgtccgagcg taccgccgat attgcccgcg ccaagaaccg
      181 taatctttga catagttttc cttctgaaat agctgtacag gagtatgagc ctcttgctca
      241 atcatttgta gcaacgtttg aatccagaaa tttattcctg tgaggaatac atttccgggc
      301 aagaggtgtg ggagactgtc gttgaccttt acagctcatt catcaattca gtcggccagg
      361 gtgggacgct ctgccaactt ccatcctcgc gcaccagcag accggcaatg tgatgtttgc
      421 gcagaaatgc cgccccatcg cgcgagccga ggataaaggc cacctttgcc gatatctcgg
      481 cttgctcgca gcgatcggtg acgaccgtga ccgaccagag atcgctttcg gctgacagac
      541 cggtgcgcgg atcgagcagg tgatggcgaa tcgtattgcc ctgcttccag tggcggcgcg
      601 caatgccgga ggtcgcgacg gctccccgga taacggggat agaccagaag cggttgcgcc
      661 caggcacgga gagcatccat tgcccatcgc cgggcgggac gccatcgacg gccaggtcgc
      721 cgcccgcgtt gacgagtgcg ggggtgatgc cacgctcgag caggctcgcc agggccgcat
      781 cgactgccat gccctttgcg atgccgccaa aatcgagctt aacgccgggg ggcagcatca
      841 cacgccgccg tatcgtatcg acgcgaatgc cgcgccatcc tcctcctggc tcgccgggga
      901 tgagtgggtc aggccgctca gccggaagct catcaaaggt gcgatcatag ccgatttgca
      961 cgagttgatc gagcatagcc gggtcgtaca tcccctgcgt cgcctgggct gctgtgagcg
     1021 cggtcgccaa cacgtgatac aggagatcgc tgacgggcag cgattcgcca gggcgggcgt
     1081 tcaggcgcga cagctcacta acgggtaaga agcggctgag ggtctgctcc cactccgcga
     1141 agagttcgcg tacaatgcgc agaccctcgt cggcctggac tgcggggagc agcagcgata
     1201 tggccgtccc catcgcccgg aactcttccc ggcgcatagt atccggggtg gtgtattcag
     1261 gtccctctga aagaggcgtg agagaaagtg gttgatccat ttatttccct gcgcagtcgt
     1321 acaactggcc tctgccgaat cctccggtgg atgtagctga ttgccattta cttgaaggca
     1381 cagaggcgca gttctgtgtc acccacgacg tcagggaact ttgccccctg ccgccaaaac
     1441 cgccaccgcc gaaaccgcca tcgccgaatc ttcgatcggg ccggtcgaag aaaccttctg
     1501 gtgggttttc agagaattga gtgggcggtt ggaacccgcc tccgatcgag ttgagcagga
     1561 agaaacgcac ggtgccgttt ttcaccagcg cggccaggct gctggttgtg aggatgggat
     1621 cgctgccact gaagccaccg agcgccatga tcggcttatt cgactccagg ataagctgat
     1681 ccgccgtttg cgaactggcg gtggcgacca ggtaggttgc gctgccctgg tttgcttcca
     1741 ggtaggtgat gagcgccgta tcggtgttcg cgctgctggt gacgccgatg ttcccaccgg
     1801 caatgccgcc aaaaccaccc tgttgggtcg ggccagccac cggaagctgc accgaaacat
     1861 cctgtagaac ggggatgcct gcccaggctg ccggtgcgat catgagcgcc agcaccccga
     1921 tacccagcgc gggcagcatg tagcgcagcc ggtcaaaggg aaagtcgaag cgccagctca
     1981 ggcgcagcac gaccagtgtg agcgcaccga cggtacagag gatggcaata gcagggatga
     2041 gccattgccc ccaggcgggg tagctgttca ggatgtggat ctgttccagg gcggtcgcga
     2101 acagcgcgag cgggagcagc cagccatgcc agctactgcg ccggtaatcc tgccacatcg
     2161 tgacgatgcc aatgccaaat agcgcggcga tggcaggcgc catttccgtc agatagtact
     2221 ggtggtagaa ggtcgcgacg ctgaagaaca cggccatggt caaaa
//
LOCUS       DASZAK010000970         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_59608, whole genome shotgun sequence.
ACCESSION   DASZAK010000970 DASZAK010000000
VERSION     DASZAK010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59608"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2380)
                     /locus_tag="VGT44_16995"
     CDS             complement(<1..>2380)
                     /locus_tag="VGT44_16995"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2582557.1"
                     /translation="QVYWIDPFGDLLISCLPGTTPACNDQLYTEFSPPEVIEKARDSL
                     TAIPIYEVGVAVDTTSSNAFNAGVIIAEPVKINGGGLTGIVAANISLNNLSVPLANVV
                     QAQAKQDRRLQISIIDEQGDLVASPDRSQILFNVLHELPGASQALQGQVNSTLGTGPD
                     GNTWLFSSVPVPGFGWAVVVQRPASEALAVVDQLHLWLIAAALLFAIGGLLFWLMLFL
                     RVIRPLHTMASQHQTLPESEQSIPVHATIVARRSDEVGGLARSLVRLERDGLEKLGEL
                     RTLLETSNAVVGSLDPHAVVKKIIGEVRRLVDVQAATVLLPDEQGVLRVLVSEGHKAH
                     YDQAVSISPENASSSAVLAMRDGRPVQKLLDGDQPSLSYEEGFRTALAIPIISRHVGS
                     IVLLVHRNRSQPFDQNEIDLLLTFANYAMLAWEHAVLYERSDVRLREVAQENELLYRQ
                     ASEEKQKLEAIMGSMSDGLVLTGSDGTVLYANAGASAIVGVSSEALERRSIGELYDAL
                     RAAAVDPQECELSLALAESLATTEAVVEISREGRRRAIHLRLFDVGDESGQVIGRGLL
                     LRDVTREREIDEFKSTLLAAVGHELRTPLAAIKGYASTLLQEDVSWSASDQRYFLQTI
                     SGEADRLALLVSNLLDLSRQEAGLLLLNRTPARVSDLVAKVIERQPGSALIADIPGDL
                     PLVNVDSARIEVVLTNLVANALAYGEGEVRIAATMRDEQVIVSIADNGPGIAADELPH
                     VFERFYRARHGRQRHSGGTGLGLTICKAFVEAHACSIWAESNSQGTTISFSLP"
BASE COUNT          430 a          732 c          772 g          446 t
ORIGIN      
        1 gggcagggag aacgaaatgg ttgtcccctg tgagttgctc tccgcccaga tcgagcaggc
       61 atgtgcttca acaaaggctt tgcagatcgt cagccccagg ccggtgccgc cagagtgtcg
      121 ctggcgtcca tgtctcgctc ggtagaagcg ctcgaagaca tgcggcagtt cgtcggcggc
      181 aatacccggt ccattatccg cgatggagac gatcacctgc tcgtcacgca tcgttgccgc
      241 gatacgcacc tcgccttcgc cgtaggccag cgcgttggca accaggttgg tgagaaccac
      301 ctcgatacgc gcgctatcca cgttgaccag gggcaggtcg ccaggaatgt ccgcgatgag
      361 ggcgctgccc ggctgtcgtt cgatgacctt tgccacaaga tcgctcaccc tggccggggt
      421 gcgattgagc agcagcaggc cggcctcctg gcgcgagagg tcgagcaggt tgctgacgag
      481 cagcgccagg cggtccgctt cgccactgat cgtttgcagg aaatagcgct ggtcgctggc
      541 agaccagctg acatcctcct ggagcagcgt ggatgcataa ccctttatgg cggccagcgg
      601 tgtgcgcaat tcgtggccaa cggcggccag cagtgtcgat ttgaactcgt cgatctcgcg
      661 ctcgcgcgtg acatcgcgca gcagcaatcc gcgtccgatg acctgtcccg attcgtcgcc
      721 cacgtcgaac aggcgcaggt ggatggcgcg ccggcgtccc tcccggctga tttcgacaac
      781 agcctctgtg gtggcgagcg attcggccag cgccagggat agctcgcatt cttgcgggtc
      841 taccgccgct gcgcgcagcg cgtcgtagag ttcgccaata gaacggcgct ccaatgcctc
      901 gctggacaca cccacgatgg cgctggctcc ggcgttggcg tagagtactg tcccatcgct
      961 gccggtcagc accagtccat cgctcatgct gcccatgatg gcttccagct tttgtttttc
     1021 ctcagacgcc tggcggtaca acagctcgtt ctcctgcgcc acctcgcgca ggcgcacgtc
     1081 actgcgctcg tagaggacgg cgtgttccca ggcgagcatg gcgtagttgg cgaatgtcag
     1141 cagcaggtcg atctcgttct ggtcgaaggg ctgagacctg ttgcgatgga ccagcagcac
     1201 gatgctgccc acgtggcggc tgatgatcgg gatggccagc gctgtgcgga agccctcttc
     1261 ataggagagc gagggttggt ctccatcgag cagtttctgc accggcctgc catcacgcat
     1321 cgccagcacc gccgaagaac tggcattctc aggcgagata gagactgcct ggtcgtaatg
     1381 ggctttgtgg ccctcgctga ccagcacacg cagcacgcct tgctcatcgg gcaggaggac
     1441 ggtggcggcc tgcacatcga ccagccgccg cacctcgccg atgatcttct tcacgacggc
     1501 gtgtggatcg agcgatccga cgacggcatt ggaggtctca agcagtgtgc gcagctcacc
     1561 gagcttttcc agcccgtcgc gttccaggcg aaccagcgag cgcgccagcc cgccgacctc
     1621 gtcgctgcgc ctggcgacga tggtggcgtg taccggaatg gattgctccg attcggggag
     1681 ggtctgatgt tggctcgcca ttgtatgcag cgggcgaatc acgcgcagga ataacatcag
     1741 ccagaacagc agtccgccga tggcgaagag cagggcagcc gcgatcagcc agagatgcaa
     1801 ctggtcaacg acggccagcg cctcgctcgc gggtcgctgc acgacgacag cccagccgaa
     1861 gccaggaaca ggcacagaac tgaagagcca ggtgttgccg tccggcccgg tgcccagcgt
     1921 tgagttgacc tgcccctgaa gcgcctggga tgcgcccggc agttcatgca gcacgttaaa
     1981 gaggatctgg ctgcggtcgg gcgaggcgac caggtcgccc tgctcgtcga taatgctgat
     2041 ctggaggcgc cggtcttgct tcgcctgcgc ctggacgacg ttcgcaagcg gtacgctgag
     2101 gttattgagc gaaatattgg ccgcgacgat gccggtcagg ccgccgccat tgatttttac
     2161 tggctccgcg atgatgacgc ccgcgttgaa ggcattggag ctggtggtat ctaccgcgac
     2221 acccacctca tagatgggga tagcggtcag cgaatcgcgc gccttttcaa tcacttcggg
     2281 aggcgagaat tcggtgtaga gctggtcgtt gcacgcggga gttgtgccgg gcaagcagga
     2341 gataagcagg tcgccgaacg ggtcgatcca gtagacctgg
//
LOCUS       DASZAK010000971         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_97240, whole genome shotgun sequence.
ACCESSION   DASZAK010000971 DASZAK010000000
VERSION     DASZAK010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97240"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..577)
                     /locus_tag="VGT44_17000"
     CDS             complement(182..577)
                     /locus_tag="VGT44_17000"
                     /inference="COORDINATES: protein motif:HMM:NF042756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6680 family protein"
                     /protein_id="HEV2582558.1"
                     /translation="MVSTIAIVAATLLGPVFAVQVQKYLERWRDQAERRKRVFKILMA
                     TRASRLSLQHIEALNLIDLEFPASRKFKRLRSAWKAYLANLNEKEPEPPADAVFYAKR
                     WDLFIDMLYEMGPVSIEECKFAADSRGRG"
     gene            1153..2085
                     /locus_tag="VGT44_17005"
     CDS             1153..2085
                     /locus_tag="VGT44_17005"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2582559.1"
                     /translation="MKKRRASWLVMALALGSLPAASASVQLKYLGTAGWEVSDGTTVV
                     LIDPYLSRLKEVTPNDGILPSDTRKLFASGDVALSDTATIDAHITRADFIVITHSHGD
                     HAMDLGYIALKTGAIVLGTERTCAVARGYGVSGDKLMQVKGGEDLKLGAISIRVIPSL
                     HGILRRAPNAPRASAPAVTVPAELKAPLHVGDFAEGGTLAYLIRIGGREILAFGSMNY
                     IEREVEGLRPDVALIGAMPERREIYQYTPRLLRALGYPPLVLPTHWDRFNVTYDVSQQ
                     PAIERLQSFLDEVKEASPKTNVIVPKYFDPITIR"
     gene            complement(2100..>2306)
                     /locus_tag="VGT44_17010"
     CDS             complement(2100..>2306)
                     /locus_tag="VGT44_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582560.1"
                     /translation="CVKEAHPQKPCHRLRQALSDGTSGTLLVRPDGADFQKFERGTTG
                     RTVMIVSQEVFFISHQSKWTIART"
BASE COUNT          450 a          705 c          635 g          516 t
ORIGIN      
        1 gaagctcaat acccagcccg cctgtgcccc tgtttacgct tcgaccgtca cctcgcgatg
       61 acgcccgcaa aactcggggt caggatggtt cgctactcct ttcctgtgcc gctctttcat
      121 cggcgacttc atgccggttt atcccggcgc acccgtatcc tcggctcagg taactgcaat
      181 cctaaccccg acctctggag tcggcagcga atttacactc ttctattgac acgggcccca
      241 tttcatagag catgtcaatg aacagatccc accgtttcgc gtaaaacacc gcatcagctg
      301 gaggctctgg ttccttctca ttcaagttgg cgaggtaagc cttccatgca ctccgcaatc
      361 tcttgaattt tcggctggca ggaaattcca aatcaatcag attcaacgct tcgatgtgct
      421 gaagcgagag gcgggacgcg cgcgtggcca tgaggatctt gaagaccctt ttccgacgct
      481 cagcttggtc gcgccagcgt tctagatact tttgcacttg aacagcaaaa actgggccga
      541 gtaacgtagc ggcgacgatg gcaatggtgc tgaccatatg ttcttatgct aactctaaag
      601 cgcccgcctt cttccgcgtt gacaccagta ctacccgccg cgcacgcgac gcaaagcatg
      661 cttcccatac gcatcgccaa gggttgcacc ggctgcgatg gggaaaacat aaacgatgtc
      721 gcaatctgga gtgagtacaa gctgctgctg tgccacctca tttgaatcgc tgtaacgatc
      781 aattcgagtt gcgagccaag gaactgccgg atcaaatacg atatgaccac catgggctct
      841 tttaacaccc gtggaactgt caatatcctc atcctcggcc cgaacaggtg gtcgcggccg
      901 cttcttgttt gccgtagacg attacgttgc tcgttggctg accgctggcg ttggtcccct
      961 tcggcgtgat ggtcatgacc gctcgtcgta agcactttcg cgcctttctt tgtcgtcgcc
     1021 tcgaccgtgt tcccgctgcg cgtgaaggcg atagtatctg cgccgttggc ttgtcctgtt
     1081 ccggtaccag tccgtgcgcc agcaatcaag taatctgccc atttctgatc atgacggttg
     1141 tagactatcc acatgaagaa acgcagagcg tcctggctgg tcatggcgct cgccctcggc
     1201 tcactaccgg ccgcatcggc gagcgtccag ctcaaatacc tgggaaccgc cggatgggag
     1261 gtgagcgatg gcaccaccgt cgtcctcatc gacccatacc tgtcgcggct gaaggaagtt
     1321 actcctaatg acggcattct tccgagcgac acccgcaagc tattcgcctc cggcgacgtc
     1381 gccttgtcgg ataccgcaac catcgacgcg catatcacgc gagcggattt catcgtgatc
     1441 acgcactcgc atggcgatca cgccatggat ctcgggtata tcgcgctgaa gaccggtgcc
     1501 atcgtccttg gtacggagcg cacctgcgcg gtcgcgcgcg gctatggcgt ctcgggcgac
     1561 aaactcatgc aggtgaaggg cggggaggac ctgaagttgg gtgcgatttc gatccgcgtc
     1621 attcccagtc tgcatggaat cctgcgccgt gcaccgaacg ctccgcgcgc gtcggccccc
     1681 gcggtcacgg tgcccgccga actcaaggcg ccgctccacg tcggcgattt cgccgagggt
     1741 ggcacgctcg cctacctgat ccggatcgga gggcgggaga ttctcgcctt cggatccatg
     1801 aactatatcg agcgcgaagt ggaaggcttg cgccccgacg tagcgctgat tggcgccatg
     1861 ccggagcggc gggaaattta ccagtacacg cccaggctat tgcgggcgct cggctacccg
     1921 ccgcttgtcc tgccgaccca ttgggaccgg ttcaacgtca cctatgacgt gagtcagcag
     1981 ccggcgatcg agcgccttca gtcgtttctc gacgaggtaa aagaggcgtc gccgaagacg
     2041 aacgtgatcg tgcccaagta tttcgacccg atcacgatcc gttgaacgtc tctacctgat
     2101 caggtgcgag cgatcgtcca ttttgattga tggcttatga aaaagacttc ctggctaact
     2161 atcatcacgg ttcggccggt ggtccccctt tcaaactttt ggaaatccgc gccgtctggg
     2221 cgcacaagta gtgttccgga tgtgccgtca ctcagggctt ggcgcaagcg atggcagggt
     2281 ttttgcgggt gcgcttcctt gacaca
//
LOCUS       DASZAK010000972         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_665200, whole genome shotgun sequence.
ACCESSION   DASZAK010000972 DASZAK010000000
VERSION     DASZAK010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665200"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VGT44_17015"
     CDS             <1..1004
                     /locus_tag="VGT44_17015"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582561.1"
                     /translation="NSVQVIGVLAASGVLYVVFFIAERFAAEPVLPLSLFRNRVFAAA
                     SLLSLLQLMTLVGLIIYLPLFLQGVLGESATYSGAVITPLTISSVIGASVAGALVATF
                     KRYQLVTIIGGIVSAIGVFLLAQMTISTSLLQAAIFMVIAGLGLGVFFSVLTLAAQNT
                     LPRTSLGVGTGAIRFLGQLGAVLGVAIVGSIVNSTVATDIVKRLPAQTVQRLTPAGVK
                     FATNPQVLVNSTYHDTVVKTAQSFAVRAATANIPPGPLHDQVAAQAIHQTITLLNQVF
                     AALKESLAVAIQHGFIAVFIFSVATVVVALFLNDIPLSTTPADVASQGDSEGLQVFF"
     gene            1548..1817
                     /locus_tag="VGT44_17020"
     CDS             1548..1817
                     /locus_tag="VGT44_17020"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2582562.1"
                     /translation="MLRQRQGYRLRGWLEEVEQHGEPELQAFARNLHKEESAVQAGLT
                     LAWSNGPTEGFIHRLKLLKRQAYGRAGVALLKQRMLFHPSDLLAA"
BASE COUNT          367 a          657 c          596 g          462 t
ORIGIN      
        1 ggaattcggt gcaggtgatt ggcgtgctgg cagcatccgg cgtactctac gtcgtcttct
       61 tcattgctga acgtttcgcc gccgagcctg tgctgccgct ctcgcttttc cgcaaccgcg
      121 tcttcgcggc ggcctcgctg ctttcgctct tgcaattgat gacgctggtc gggctgatca
      181 tctacctgcc gctcttcctg caaggtgtgc tgggcgaatc ggccacctac tccggggcgg
      241 tcattacgcc gctgaccatt agctccgtaa taggtgcgtc ggtggcgggc gccctggtcg
      301 ccacgttcaa acgctaccag ctcgttacga tcatcggcgg catcgtgtca gccatcggcg
      361 tcttcctgct ggcgcagatg acgatctcga cgagcctgct gcaggccgcg atcttcatgg
      421 tcatcgcggg cctcggccta ggcgtcttct tctcggtgct gacactggcc gcgcagaaca
      481 cgctgccgcg caccagcctg ggcgtcggca ccggcgcgat acgcttcctg ggccagcttg
      541 gcgcagtcct gggcgttgcc atcgtcggct cgatcgtcaa cagcacggtt gccaccgaca
      601 tcgtgaagcg cctccccgcg caaacagtgc agaggcttac gcccgccggg gtgaaatttg
      661 ctaccaaccc gcaggtgctg gtcaactcga cctaccacga tacggtggtg aaaacggcgc
      721 aatcgttcgc cgtgcgcgcc gcgactgcca acatcccgcc cggcccgctg cacgaccagg
      781 tcgcggcgca ggccattcac cagaccatca cgctgctgaa ccaggtcttc gccgcgctga
      841 aagaatcgct ggccgtcgcc atccagcacg gcttcatcgc ggtgttcatc ttcagtgtcg
      901 caactgtggt ggtggcgctc ttcctcaatg atattccact atccacgaca cctgctgacg
      961 tggcttccca gggcgactca gaaggcctgc aagtgttttt ttagcgaaag gatacaacag
     1021 aatttctcaa gtagcttgta gggtacagcg attcacagtg gtggataatt cgtactgctc
     1081 tcccactcgc ttgacagcat gggagagaca ccgtatcctt gcttctcgag gagctctccc
     1141 ttctcctcag ttcaagcagc gagggttcgt atggctcttc ctctgttcgt gctcatggtc
     1201 tgtctcctcc tctctttggt gctgctttgg cgtcatgact ggctctatct ccggccctcc
     1261 tcacgaggag gagccaagcg cggcaccttc ccccgtctgt tcccacggga tgcgcaacga
     1321 gttttacctc aacagcaatc cggtttgaga gcattcttca tcgcttctct ttttcgagaa
     1381 agagaagatg tctgaaccct tgtccgtgga caagcgcgag tgttgaacga gcctgccaag
     1441 gtgcttcaat cgaaaataca gctgcgctct gcatcctgtg ggcgcggctg atccaagccg
     1501 acgagtcgct ggctcgcacg gcaggccaag tggaggcctt ttgtcatatg ctgcgtcaac
     1561 gccaaggata tcggctgcgg gggtggctgg aagaagtgga gcagcatggc gagcctgaac
     1621 tgcaggcatt tgcacgcaat ctgcacaaag aggagtctgc cgtacaagca gggctaactc
     1681 tggcgtggag caacgggcca acggaaggct ttatccatcg cctcaaactg ctgaagcggc
     1741 aagcgtatgg ccgggcaggc gtcgccttgc tcaaacagcg gatgctcttc catccctctg
     1801 acctactagc cgcttgactc tcatgaccac ccgcccgatc agcgagaagg cgcaagaccg
     1861 caggtctggg tggcgaggtt tcctacttct gttcctcttt tacgcccatg gcctcggctg
     1921 gtccagccgc gatccacgtg caaccgcggc gactcagccg agttcttggg ccagcccgat
     1981 gagcagtcct tcaggtccgc ggatgtagca gagccgatac gcgtcttggt actggaccac
     2041 ttcgaggctt tcaagctctg cgccgtgctt gcggagccgg ac
//
LOCUS       DASZAK010000973         4595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_200703, whole genome shotgun sequence.
ACCESSION   DASZAK010000973 DASZAK010000000
VERSION     DASZAK010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200703"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1627)
                     /locus_tag="VGT44_17025"
     CDS             complement(<1..1627)
                     /locus_tag="VGT44_17025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEV2582563.1"
                     /translation="MKHLRTDRPAAWPMSIIASIVLLGMLLAGCSFPGTSNTTASTPG
                     ANQHTPAGGGGVNPTPTPHPITSKPNTGKVNGGQPLPQNEIKPLTFNLAYNDAAMEQD
                     VAQMYTPGSPNYHQYLTPDQIVQRYALSDAQLQVVENWLRGNGFTIVSADPLRLGIQV
                     RASVATIEKALNVQLRLYTINLFGTSYSFFVQETDPVITGPVSAYIQSVVGLDSFAFP
                     IFNPPGSITRAFALQAADCAKYGARQTLTSSNLASAYQLDTLHSHGLMGQGMTIGVAE
                     FGDPFDPKDVANYAACAGAGVPNIQIVNVDGQLAPGSGEGEATLDLEMIAGLAPKAQI
                     LDYQASLDTTSFAQSLVDVFNKVASDDRVQVLSVSYGTGESSFSISEQDAINRSLRNL
                     AAEGISVFISSGDCAAFSNRVTNIAMVSFPASAPYAIAVGGTHLQVNSKGARTSEDVW
                     SDRDFAPVCSNEWGSGGGVSQNSDFKRPAWQVGPGTTNHYDGTSALVFTNNLNPVVAP
                     NGLRQVPDVAAAAFPNIAMYYKGAWIAVGGTSAA"
     gene            2313..2594
                     /locus_tag="VGT44_17030"
     CDS             2313..2594
                     /locus_tag="VGT44_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582564.1"
                     /translation="MNSSMINKIAKAKRYAEEPERIQFTRFEATFRGENDVHTTNYDN
                     GEWHCTCRFFHDWGDCSHTMAMQRVLGVTIPAAYRTGIPAGIGTGPLMH"
     gene            complement(2806..3336)
                     /locus_tag="VGT44_17035"
     CDS             complement(2806..3336)
                     /locus_tag="VGT44_17035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582565.1"
                     /translation="MPKKSASVRGNAQRNRAKLQKNIQLVRPVPATAESESEINEEVE
                     STSAVATTTAPSLVAEVEEEEATEEVVPTPAREPSKRTRSSRPSDTSARAATSPVAPA
                     SPASSTPAAPASASARLAARRQQQQQPVQKTRQSSLVTAEHYAYVRRDLIFIAILAIL
                     MFGTLVVLHFVPAIGG"
     gene            3768..>4595
                     /locus_tag="VGT44_17040"
     CDS             3768..>4595
                     /locus_tag="VGT44_17040"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEV2582566.1"
                     /translation="MLLQATIACILLAGCMGTPSHPQTLLPGAGPYRLPVDTFPINSP
                     VATTGCGQAPSIPQGTSVDMTIAAHPAEALGNRTRSYLLHLPKHYNNAISYPVILIFH
                     GFGGTAAGMERGSAFSTLADAQGFIAVYPQGLPQPHTDKPFWAEIGPIDYGVDDVLFV
                     SDILNDLQQKFCVDAHRLYATGFSNGGGLTTLLACRLAGRVAAFAPISGNDYSIPGGC
                     HPGRPVALLNMHGSADPLLPYNGTPPGSSPDWPLPSLPQYLRAWAARDGCATGPVIFL
                     "
BASE COUNT          971 a         1305 c         1305 g         1014 t
ORIGIN      
        1 aggccgcgct ggtgccaccg acagcgatcc acgcgccttt atagtacata gcgatattgg
       61 ggaaggccgc cgccgcgaca tcgggcacct ggcgcaggcc gttcggtgcc acaacggggt
      121 tcaggttatt cgtgaagacc agcgcgctcg tgccgtcgta gtgattggta gtgccggggc
      181 cgacctgcca ggcaggacgt ttgaaatcgc tgttctgact gacaccgccg ccgctgcccc
      241 attcgttcga gcaaaccggc gcgaagtcgc gatcgctcca cacatcctcg cttgtgcgcg
      301 cgcccttgct attgacttgc aggtgcgtac cgcctacagc gatagcgtaa ggcgcgctgg
      361 ccgggaagga aaccatcgcg atgttggtga ctcgattgga gaaggctgcg cagtcgccgc
      421 tgctgataaa gaccgaaatg ccctcggcgg ccaggttgcg caggctgcgg ttaatggcat
      481 cctgctcgga gatgctgaag gaggactcgc ccgtgccata gctgacgctc agcacctgca
      541 cacggtcatc cgaggccact ttgttgaaga catcgacgag cgattgcgca aaggaagtag
      601 tatccaggct ggcctggtag tcgagaatct gcgctttggg cgcgagaccg gcgatcatct
      661 ccaggtcgag cgtggcctcg ccttcgccgg agccgggcgc gagttgtcca tcgacattaa
      721 cgatctggat attggggacg ccagcccccg cgcaggcggc gtagttggcg acatcttttg
      781 gatcaaaggg gtcgccgaat tccgctacgc caatcgtcat gccctggccc atcaggccgt
      841 gcgagtggag ggtatcaagc tgatacgcgc tcgcaaggtt gctgctcgtc agcgtctgcc
      901 gcgcgccata cttcgcgcaa tccgccgcct ggagcgcgaa cgcgcgggta atactgcccg
      961 gcggattgaa aatggggaag gcgaaactat ccaggccgac gaccgactgg atataggccg
     1021 agacagggcc ggtgatgacc ggatcggttt cctggacgaa gaacgaatag ctcgtcccaa
     1081 acaggttgat cgtatacagg cgcagttgta cattcagcgc tttttctatc gtggcgacac
     1141 tggcccgcac ctgaatgccg agccgcagtg gatcggcgct cacgatggtg aacccgtttc
     1201 ccctcagcca gttctcgacc acttgcaact gcgcatcaga gagggcgtag cgctgcacga
     1261 tctggtcggg ggtgagatac tggtgatagt tcggcgagcc gggcgtgtac atctgcgcca
     1321 catcttgctc catcgccgca tcattgtacg ccaggttgaa ggtaaggggc ttgatttcgt
     1381 tctgcggcag tggttggccg ccattgactt tgccggtgtt gggtttgctg gtgatcggat
     1441 gcggcgtggg cgtcggattg acaccaccgc cgccggccgg cgtatgctga ttcgcgccgg
     1501 gagtcgaggc cgtcgtattg gacgtgccag ggaacgaaca cccggccaaa agcatcccca
     1561 gcaggacaat actcgcaatg attgacattg gccatgcggc aggccggtcg gttcgtagat
     1621 gcttcactca atacctccgt ataaaaatga attttgatat ccctaaggcg tttctcacga
     1681 ttagtgtgct atcctctaca attggcagta tagtcggatt gtcaattgcg tacaatgaag
     1741 aggacgattg cgttactgtg aaatcggtta caatttgccg ttccatgaca caattctgtc
     1801 acagggcagt ttcgactgct aaaagggtgg acaatcgaag ccggatatgg tataatcaag
     1861 ccattcagac aaggaaattc gggacagact atcaaggtca cgagggtatt tgtctatgga
     1921 cacagttgtt tcaccatcag atgtcgcatc actggcaaag gcagtggcgg ctgatgatgc
     1981 gaatagacga cactagggaa ggtttgccct ggtgtttttt tatacccttt aatttcattc
     2041 gaacattcat tatcacgcaa ggttgccggg ctcatcgatt ttttcttcat tctgtgggcc
     2101 agagttagag gcgctccaac tggatgagcg tacccaagtt gcgaaaggag gaacgaagaa
     2161 gctactcacg agctaacggc gtaaaacggc aaacacccac aacgcaggcc agcattcggt
     2221 aataatgagg ctgcgtatct cttacgtata cagtacgttt gatcgacaag tggtgcggag
     2281 tggatcgcta gatttgaagg agatgaccga cagtgaattc aagtatgatc aacaagatcg
     2341 cgaaagccaa acggtacgcg gaagaacccg aacgaatcca gttcacacgc tttgaagcca
     2401 ccttccgcgg cgaaaacgat gttcacacca cgaactatga taacggggag tggcattgta
     2461 cctgtcgttt cttccatgac tggggggatt gcagccatac tatggcgatg cagcgagtcc
     2521 tgggcgtaac tattccggca gcctatcgca cgggtatccc ggcgggcata ggcaccggcc
     2581 cgttgatgca ctaaccccgg caaacataca tctggctgtg gggcgttctc accccatact
     2641 ccagccaaca atgcacacaa agagaagcgc tgatatcgat tgaagaaaca ccagcgcttc
     2701 tctttttatc tggattcaca ctatttcctc atgcttcata aggctcgccc ctacagacac
     2761 catgaccctg ttcggtcgtc aaaagtcata aggggggatc acttgttacc caccaatggc
     2821 ggggacgaaa tgcagaacga ccagtgtgcc aaacatcaga attgccagaa tagctataaa
     2881 aatcaggtcg cgacgcacat aggcataatg ctccgctgtt accaggctag actggcgcgt
     2941 cttctgcacg ggctgctgtt gttgctggcg acgagccgcc aggcgcgctg aggcgctggc
     3001 tggtgcagca ggtgttgaag atgctggaga ggctggggca actggagagg tcgcagccct
     3061 tgcggaagta tccgaggggc gcgaggacct ggttctcttc gagggctcgc gcgcgggtgt
     3121 tgggacgacc tcctcggtcg cttcttcctc ttctacctca gcgacgagtg acggggcagt
     3181 ggtggtggca acggctgagg tactctccac ctcttcattg atctcacttt cgctttccgc
     3241 cgtagccggg acggggcgca caagctgaat gttcttctgt agtttcgctc tattgcgctg
     3301 cgcattgcca cgcacactgg ctgacttctt cggcatcgtt ctttcactcc ctgcccaccg
     3361 gcctgcaact ctggcacacg ggctattcta gtaatatgct ctttgtaggt atcgtcctga
     3421 tataatctga gacgctgtat aagtcctaac tatcgcgttc cgttcccaaa atattctgca
     3481 tggatgacgg catccatcgc ttctgcctca ttatacacga aaggtggtta tgagtcgagg
     3541 ggtactacat gatccatgga ccgacgcaac gtgagacatg gcgcaccctt gcggtcgcca
     3601 tgtctggtct tatgcgcaat agtttgcctc gccttgcgtc ttgatgtata gagtggtaat
     3661 ccccgactga accatactac tctatctacc ccgccaggag ggtctgtcaa tgcggcgaga
     3721 tatcctaaca tagtcagggc agtcttccaa gaagctcttt gctgtgtatg ctccttcagg
     3781 ccacaatcgc ctgcatcctg ctggcgggct gtatgggtac cccgtcgcac cctcagacgc
     3841 ttttgccagg ggcggggcca taccggttgc cggtcgatac gtttccgatc aattccccgg
     3901 tggccacaac tggatgcggc caggccccgt caattcctca gggaacgagc gtcgatatga
     3961 ccattgccgc acacccggcg gaagcgctcg gcaaccgcac ccgaagctac ctgctgcatc
     4021 tccccaaaca ctacaataat gccatttcct atccggtaat cctcatcttc cacggcttcg
     4081 gtggaacagc agcaggcatg gaacgcgggt ccgccttttc cacactcgcg gacgcgcagg
     4141 gcttcatcgc cgtctaccca cagggactgc cccaaccgca taccgataag cctttctggg
     4201 ccgagattgg cccgattgac tatggagttg atgacgtact gttcgtcagc gatattctga
     4261 atgatctaca acaaaagttt tgtgttgatg cccatcgact ctatgcgaca ggtttctcaa
     4321 acggcggcgg cctgacgacg ctgctggcgt gcaggctggc ggggcgggtt gccgccttcg
     4381 ctcctatctc cggcaatgac tactccatcc ccggcggctg ccatccgggg cgaccggtag
     4441 cgcttctcaa catgcacgga agcgccgacc cattactgcc ctacaacggc acgccgcccg
     4501 gctccagccc cgattggccg ctgccttcgc tcccgcaata cctgcgtgcc tgggcagcgc
     4561 gtgacggctg cgccaccggc ccggtcatct ttcta
//
LOCUS       DASZAK010000974         2718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_308850, whole genome shotgun sequence.
ACCESSION   DASZAK010000974 DASZAK010000000
VERSION     DASZAK010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308850"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VGT44_17045"
     CDS             <1..1108
                     /locus_tag="VGT44_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HEV2582567.1"
                     /translation="DGLERYDSARPSAAIQEFLEDLSNWYLRRSRERFWKQEMDNDKV
                     AAYLTLYECLTTLVTLLAPFVPFVTEELYQNLVRNVNPAAPASVHLCDWPQVNEDLVD
                     EQLQRETRLVMRVVGLGRSAREKAQIKVRQPLNALYARVKHWDEEEAMLRLKGQVLEE
                     LNIKQLHLMSIDSDMLGYTLKPHVKVLGPKYGPLVQKILAAFKALDAHGAQRAAEVLN
                     ETGTLSFTIDGQQLVLTRDEVEVIATARPGFVAAEEHGYVVALETTITPQLREEGLVR
                     DLTHYVQDMRKKANLNIEDHIVLALYTGEELADLLNRQRKTLQAETLADTLHIHATQQ
                     PEHVCNEVYRESISPTSLKKLEQYSLEVVIGKPS"
     gene            1123..1974
                     /locus_tag="VGT44_17050"
     CDS             1123..1974
                     /locus_tag="VGT44_17050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2582568.1"
                     /translation="MHLKVNGTRLFFDVVGSHLEPVGDAMRPKPTLIMLHGGPGFDHS
                     YFRPAMDPLGEVAQVIYLDERGQGRSARHSNDYYQLGIMADDVAAFFDELSIEKPVVL
                     GDSFGGFVALSLAVRHPDLAAGLILISTLPWWNGYDLDALEALAGKDLREVAARELAG
                     EASEEDMRRFFAEVFPYYYMQFKPEYLSMLNRTIMNEDIDLYMNRSLRTEYDMRPHLA
                     GITIPSLVLHGRHDWVTPFAEAEKMAQALPQAHLHVLEHSGHMVQDDEPEEVIKAIRE
                     FLLGIGA"
     gene            complement(1999..>2718)
                     /locus_tag="VGT44_17055"
     CDS             complement(1999..>2718)
                     /locus_tag="VGT44_17055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582569.1"
                     /translation="SAVSGFSRQWRTSSDQARTDAQRREVVRQAARLMILGGLGLTLL
                     AFVTSPPASASTPWTSARYLVGFLIALPALLFPLWEMGRNVKNGRIWIDRLKSGLRVA
                     LLLLIFAASLLGTIDTFATQLSLSRASFSRVNGLIGGLLQLGATHIYANSDDCTRIAF
                     LSDERVICAVLDQGLNPGLDRYFPYRAQVASWPQPFYVFLEGSPQAVLFQIKAAQQHI
                     AYSTFKINGYDVFEPSRRVLP"
BASE COUNT          610 a          781 c          817 g          510 t
ORIGIN      
        1 cgatggactg gaacgctacg acagcgccag gccgagcgcg gccatccagg aattcctgga
       61 agacctgtcg aactggtacc tgcggcgcag tcgcgagcgc ttctggaaac aggagatgga
      121 caacgacaaa gtcgcggcat acctgacgct ctacgaatgc ctgactacgc tcgtgaccct
      181 gctcgcgcct tttgtgccat tcgtgactga agagctgtac cagaacctgg tgcgtaacgt
      241 caacccggcg gctcccgcca gtgtgcatct ttgcgactgg ccgcaggtga acgaagacct
      301 ggtggatgag caactgcaac gcgagacgcg gctggtgatg cgcgtcgtcg gcctgggccg
      361 ctcagcgcgc gagaaggcgc agatcaaggt gcgccagccg ctcaacgccc tctacgcgcg
      421 cgtcaagcat tgggacgaag aagaggcgat gctgcgcttg aaagggcagg tgcttgaaga
      481 actcaatatc aagcagttac acctgatgag catcgacagc gatatgctgg gctacacgct
      541 caagccccac gtcaaggtgc tggggccaaa gtacggcccg ctggtgcaga agattctggc
      601 cgcgttcaag gcgctcgacg cacatggcgc acagagggca gcggaagtcc tcaatgaaac
      661 aggaacgctg agcttcacca tcgacggcca gcagcttgtc ctgacgcgcg atgaggtgga
      721 ggtaattgcc accgcgcgcc ccggattcgt ggcagcggaa gagcatggct acgtcgtcgc
      781 gcttgaaacc accatcacgc cgcaactgcg cgaggaggga ctggtgcgcg atctgaccca
      841 ttacgtgcaa gacatgcgga aaaaggcgaa tctcaacatc gaagatcata tcgtactggc
      901 cctgtatacc ggtgaggaac ttgccgacct gctcaatcgc cagcgcaaaa cgctccaggc
      961 ggagacgctg gccgatacgc tgcacattca cgcgacgcag cagcccgagc atgtgtgtaa
     1021 cgaggtctat cgcgagtcca tctcgcccac atcgctcaaa aagctggagc agtatagcct
     1081 ggaagtggta ataggcaagc cctcctaacg cggagaaaat acatgcacct gaaagtcaac
     1141 ggcacccgac tctttttcga cgttgtaggc agccatctcg aacctgtcgg cgacgcgatg
     1201 agaccgaagc cgaccctcat catgctacat ggcggccccg gcttcgatca ctcgtacttt
     1261 cgcccggcga tggacccgct gggcgaagtg gcacaggtta tctacctgga tgagcgcggg
     1321 cagggtcgct cggctcgcca ctccaacgac tactaccagt tgggtattat ggccgatgat
     1381 gtcgcagcct tctttgacga gctgagtatc gagaagccgg tcgtgctggg cgattctttc
     1441 ggcgggtttg tggcgctgag cctggcggtg cgccatcccg acctggctgc cgggctgatc
     1501 ctgatctcga cgctgccctg gtggaacggc tacgatctgg atgccctgga agcgctggcc
     1561 gggaaggatc tgcgcgaggt ggcggcgcgt gagctggcgg gcgaggcctc ggaggaggat
     1621 atgcgccgtt tcttcgccga ggtctttccc tactactaca tgcagttcaa accggaatat
     1681 ctttccatgc tgaaccggac catcatgaac gaggatatcg acctgtatat gaaccgctca
     1741 ctgcgaaccg agtatgacat gcgaccccac ctggccggca ttaccattcc ttcgctcgtg
     1801 ctgcatggcc gacacgattg ggtgacgccc ttcgccgagg cggagaagat ggcgcaggct
     1861 ctcccccagg cccacctcca cgtcctggag cattccgggc atatggtgca ggacgacgag
     1921 ccggaagaag tgatcaaggc gatacgtgag tttttgcttg gcattggcgc ttgatctgtc
     1981 ggaacgtgtc tgaatatcct atggtagaac acgccgcgac ggttcgaaca catcatatcc
     2041 attaatcttg aacgtgctat aggcaatatg ctgctgagcc gctttgatct gaaataagac
     2101 cgcctggggt gaaccctcaa gaaagacata gaatggctgc ggccagcttg cgacctgggc
     2161 gcgatagggg aagtagcggt caagccccgg attcaacccc tggtcaagca cggcgcagat
     2221 cacccgttcg tcgctgagaa atgcgatacg ggtacagtcg tcggagttgg catagatatg
     2281 cgtcgcgccc agttgtaaca gaccgccaat cagtccattt accctgctaa agctggcgcg
     2341 cgaaagagac aactgcgtcg cgaatgtatc aatggttccc agtaagctgg cggcaaagat
     2401 caagagcagt aaggcaactc gcagcccgga cttaagccta tctatccaga tccgcccgtt
     2461 tttcacgttt cttcccattt cccaaagggg aaagagcagc gcgggcaaag caatcaaaaa
     2521 tccgacgaga tagcgcgccg aagtccaggg ggtagatgct gatgctggtg gtgaggtgac
     2581 aaaggcgagc agggtcaaac ccaacccgcc gagaatcatt aagcgagcgg cctggcgaac
     2641 tacttcacgc cgttgggcgt cggttcgtgc ctggtcggac gacgttcgcc attgtcgact
     2701 gaagccactg accgcgct
//
LOCUS       DASZAK010000975         3289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_167164, whole genome shotgun sequence.
ACCESSION   DASZAK010000975 DASZAK010000000
VERSION     DASZAK010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167164"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1716)
                     /locus_tag="VGT44_17060"
     CDS             complement(<1..1716)
                     /locus_tag="VGT44_17060"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEV2582570.1"
                     /translation="MQSSEDYQQYGPLKIVVCIKQVPLLSALRFDPETHRLVREGVPL
                     EVNELDVYALAEAVRLRDLHGGEVIAMTMGPPQAHTALATAIAIGVNRAIHLNDRAFG
                     GADTFATARALAMAIKREQPDLVFCGRHSIDAETAQVGPEIAEMLDIPQISAAQKVDM
                     AVQNGKRAVIVVRETDDGSETLAAPLPALVTTAERLNEGIWPDEAAIQSAGAQAERYQ
                     VATAADLSPDSSLFGQPGSPTWVAGVEPDSYTREGRVLSGDDPANLVDELLSVLEAKG
                     LLAHDAAAQDKEYQSDQATTPVALSRRQGDALPDRAIWVIAERSPQGIRKVTLELLGK
                     GAELAADLHGELAALLFGGADAEQHAVTLAAYGAERVYIAADPALEDYSTEGYTAVVS
                     SAIRNYRPAVVLLGSTTNGRDLAPRVAARLGIGLTGDCIDLRIDEQSRLVQYKPAFGN
                     QILSFILSKTPPAMSTLRPGMLRQVSPDFSRTPQITYLPTAGVAEQIRTTILGRIERD
                     TGTQALESARIIVGIGMGVGEPPHYAEVYRLAELLRAGIGATRNVTDQGWLSKHMQIG
                     LTGRAV"
     gene            2378..>3289
                     /locus_tag="VGT44_17065"
     CDS             2378..>3289
                     /locus_tag="VGT44_17065"
                     /inference="COORDINATES: protein motif:HMM:NF018218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HEV2582571.1"
                     /translation="MGNKGRRAPSGKGRGARHKSVVTYARTSAQFIRTHKQEFLAGGL
                     VIILVVLLFGVVSYFQPPGTNAAQSGETTIDYSAFIAQVKAGNVLAATIRGSDVHGLL
                     ISPLAQSNGSATATPTITATQRATDLSTYSRYLGTNSGWANSQQTPAIAPSRLVYTRE
                     PDAGDPTLMPLLVNSHVIVTTQPPAQPSLLLTLLWRILPFVFFILAALFLLAPRNPRR
                     AAGGSDEHFQQMGKSKARRFEQGLEMKEKKKSKNEPSFNMLKPASPTPGGTNKADAAA
                     QSKRLPPPVTFQDVAGIDEVREELEEIV"
BASE COUNT          701 a          971 c          919 g          698 t
ORIGIN      
        1 aacggcgcgt cccgtcagcc cgatctgcat atgtttcgac agccagccct ggtccgttac
       61 gttgcgcgtc gcgccgatac ctgcgcgcag cagttccgcc agccgataga cttcggcgta
      121 gtgtggcggc tcccccacgc ccatgccgat gccgacgatg atgcgcgccg actctaacgc
      181 ctgcgtgccg gtatctcgct caatgcggcc caggatcgtc gtgcgtatct gctcggctac
      241 acccgctgtt ggaaggtaag tgatctgcgg cgtgcgcgaa aaatcggggc tgacctggcg
      301 tagcatccca gggcgcaggg tagacatggc gggcggcgtc ttggagagaa tgaatgaaag
      361 aatctggttg ccgaaggccg gtttgtactg caccagccgc gactgttcat cgatgcgcag
      421 gtcgatgcag tcgcccgtca ggccgatccc caggcgagcg gccacgcgcg gcgcaaggtc
      481 gcgcccattg gtcgttgaac ccagcagcac aaccgccggt cgatagtttc ttatagccga
      541 agagactaca gccgtgtagc cctcggtgga atagtcttcg agcgcggggt cggcggcgat
      601 atacacccgt tccgctccat aggccgccaa cgtcaccgcg tgctgctccg catccgcgcc
      661 accgaacagc aaggccgcca gttcgccatg caagtccgct gccagttctg cgcccttgcc
      721 aagcagttct aacgtcactt tgcggatgcc ctgtggggag cgctccgcaa tcacccaaat
      781 ggctctgtct ggaagtgcat cgccctgtcg ccgcgacagg gctactggtg tcgttgcctg
      841 gtcgctttgg tactctttat cttgcgccgc tgcatcgtgg gcaagcaacc ccttcgcctc
      901 caggacgctg agaagctcgt caaccaggtt cgccgggtcg tcaccggaca acacgcgacc
      961 ttcgcgcgtg tagctatccg gctcgacgcc agctacccag gtgggagagc ctggctgccc
     1021 gaagagcgag ctatcgggac tcaggtcggc agccgtcgcc acctggtagc gttctgcttg
     1081 cgcgccggcg ctctgaatag ccgcttcatc gggccagatg ccctcattca agcgttccgc
     1141 cgtcgtcacc agcgcgggca atggcgcggc caacgtttcg cttccatcgt cggtttcgcg
     1201 taccacaatc acggcacgtt tgccgttctg aacagccata tcgaccttct gcgcggcgct
     1261 gatctgtggg atgtccaaca tttcagcaat ttcgggaccg acctgcgctg tttccgcgtc
     1321 gatgctgtgc cgcccgcaga agaccaggtc gggctgctcc cgtttgatgg ccattgccag
     1381 ggcgcgcgcc gttgcgaagg tgtccgctcc gccaaaggca cggtcattca ggtggatggc
     1441 cctattcacg ccgatcgcga tagcagtcgc cagcgccgtg tgcgcctgtg gcggccccat
     1501 cgtcatcgca atcacttccc caccatgcag gtcgcgcagc cgcaccgctt ccgccagcgc
     1561 atacacatcc agttcgttga cctccagcgg tacgccctca cgtaccaacc ggtgtgtctc
     1621 cgggtcgaaa cgcagcgcgg agagcagcgg cacctgtttg atgcacacga ctatttttaa
     1681 tggaccatac tgttgataat cttcacttga ctgcattttt gactcccaca tctccagccg
     1741 atccgtagat gcttcgctgc gctcagcatg acaaaacatt gcttatggtc gttgcctgcg
     1801 acagcatcat tataagcaat gatagaggtc tacaacaaac gaatgatagc cccacagcca
     1861 attcgtactc ttctctcgct tttgtgaccg attttgttac ctccaacgta ttacaatcag
     1921 gatacacgtt gacacgtgaa acgcaacata gaaataaatt gatgtgctgc gttataatga
     1981 gaagatagta ctgaaaacat ttctgtattc gcattgtttg cggtacaatc tgtacgctat
     2041 agtatagtag acgttagaat tacggtgtcc atgtcgatac cgattgcgca gtagccactg
     2101 ttgtcgctgt tgtcactcta atcacagcag gctgttttca ttacgggtat catttgtcct
     2161 ttttcctgca aattatcgcg ggtcaaaaga aggattctcc tggggaagaa caaggagttc
     2221 gacgcgccag tgcaggcaca ggagaagcaa actttttctg tagagtgaag aagcgcaagc
     2281 tactgcatag gtcagtgcgg aggagcaggg atgaagttga tggtaagggg gagtgctacc
     2341 tgcgaggctg gtctgggcgt tgtcaagggg tacgtacatg ggcaataagg ggcgtcgcgc
     2401 gccgtcagga aaagggcgag gagcgcggca taagagcgtc gtaacgtatg cgcgaacaag
     2461 cgcgcagttc ataagaacgc ataaacagga atttctagcg ggcgggctgg tcattattct
     2521 ggtggtgctg ctcttcggag tagtgagcta tttccaacca ccagggacga acgccgcgca
     2581 gagcggcgaa acaaccatcg attacagcgc gttcatcgcc caggtgaagg ccggtaatgt
     2641 gctggcagcc acaattcgcg gaagtgatgt gcatgggcta ttgattagcc cactcgcgca
     2701 atccaacggc agcgccacag cgacgccgac gatcaccgct acccagcgcg ccaccgactt
     2761 atcgacgtat agtcgctatc tcggaacaaa ttcaggttgg gctaattcgc agcaaacgcc
     2821 tgcaatcgct ccatcgcggc tggtatatac tcgcgagccg gatgccggcg atcccacgtt
     2881 aatgccgtta ctcgtcaact cccacgtcat agtaacaacg cagccacctg cgcagccttc
     2941 gctattgctc acactactct ggcggattct tccatttgtc tttttcatcc tcgctgcact
     3001 cttcctgctt gcgcctcgca atcctcgtcg cgcagcaggc gggtcagacg agcatttcca
     3061 gcaaatgggt aagagcaaag caagacgttt tgagcagggg ttagaaatga aagaaaaaaa
     3121 gaaatcgaaa aacgaaccat cattcaatat gctgaaaccc gcctcgccca ctcccggcgg
     3181 gacgaataaa gctgacgcgg cagcccagag caagcgcctg ccaccacctg tcaccttcca
     3241 ggatgtagcg ggcatcgacg aggtgcgcga agaattggaa gagattgtg
//
LOCUS       DASZAK010000976         5295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_665313, whole genome shotgun sequence.
ACCESSION   DASZAK010000976 DASZAK010000000
VERSION     DASZAK010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665313"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..694
                     /locus_tag="VGT44_17070"
     CDS             194..694
                     /locus_tag="VGT44_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582572.1"
                     /translation="MSNPYDPTQNAYPPSGPYNNNDGPYATPQQPFTGYPPQPDSGYP
                     QPGYTPQQPYYPTDQPYYPPANTPGMYPPQQQQIGYIGYMAAPAEPTNGPGIASLVLG
                     IVGLFICWIPFIGLAMPIVGLVLASVGMKRLSGKGLAIAGLVLSIIALVLGGCVSAAV
                     ITSIHY"
     gene            complement(675..2039)
                     /locus_tag="VGT44_17075"
     CDS             complement(675..2039)
                     /locus_tag="VGT44_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582573.1"
                     /translation="MNVFIDFARQFGRFQRNAKLYLINTALGGVTTGILLILYNLYLT
                     SLGYGTDFVGLVLFIGTIGGGIAIFPAGYIIDRVRAKPILIASNLLIALVGIGQILFR
                     QPVPLLVTSFLAGIALVFTVVINAPFLTRNSVPEERPGLFSVNISLGLITLVVGEVVG
                     GALPVWFRSQGWLMASFPSWASALLAGQPEARSYQLALLFAGLIAAPSLVPLFLMREA
                     RRPSIGRPASDRPASGDPSPGRPQGVSLHIDKTHPTGWVGGMVRTPFFFLALVYVITG
                     LGAGLVIPYLNIFFVHHLHASSVLFGLVDGGANAATALLTLVAPWLAARVGRIRAIVL
                     TRLLSIPLLLTIGLVGIVPLAALLYPFRQGIMDMSNGVLQVYSMEVVEERYRGLANSA
                     YQAAFQVPWALTASLGGLLIAHFGYTLPFLLTALCYMAAIGILWGRFSVKSPQTNALS
                     SELK"
     gene            complement(2023..2622)
                     /locus_tag="VGT44_17080"
     CDS             complement(2023..2622)
                     /locus_tag="VGT44_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582574.1"
                     /translation="MHQLMSVTLADVLAPWGQAAATVLAIFLFVNILVGLALTAAFAF
                     LFAWVREKAELIKKLRPTVDMVNKAMAAPESVVPTGKTQERLAQAVQRVQAFEVSKKL
                     EQARQRTQSISAQVEQRADRVAETMIEIRARTVMVKGMAKAFFLPGLVERPRLTTRAR
                     VLLPEVVEMPAGGDGLAVEPASAARDERQLVEIGNERLH"
     gene            complement(2625..3137)
                     /locus_tag="VGT44_17085"
     CDS             complement(2625..3137)
                     /locus_tag="VGT44_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582575.1"
                     /translation="MKKARNYLLIVSVSVLILLVAGFILAGIFDKLLDVLYISLIVLA
                     AFSLISTALLVYLLLSLIRTVMTVSAEMKPLLESVNQTVSTVRGSMEDTLETVKDTAR
                     SASQTVSTVGATARLTNQAVGPTVRVAALVVAGREAVRIFVGKGHTRRRYEERRKEQM
                     EMMNAASRGD"
     gene            3337..3702
                     /locus_tag="VGT44_17090"
     CDS             3337..3702
                     /locus_tag="VGT44_17090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582576.1"
                     /translation="MHALALVLADGVTIKVGSNTWTFGTNLIVYLIIAAIIGFLAEFL
                     VGWRLPFGFVGAIIAALIGIWLMTQVIQFSISGFNDPTVDGVPLVHALIGAIIFVAIW
                     HLITYPAYRGRRRYYYRSR"
     gene            4106..4642
                     /locus_tag="VGT44_17095"
     CDS             4106..4642
                     /locus_tag="VGT44_17095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2582577.1"
                     /translation="MARAETQETSWSFDQIYDEYKTPIYNYVYHLVGDREQADDLTQD
                     TFLKAFRALPKMDGNLKLSAWLYRIATNTAYDALRRRKLIAWLPWQELDHEPADVEGA
                     DPQEIYGTTELVRAALRRMPNQYRAALLLYTQEGFSYSEIARALNIAESGVKMYLSRA
                     RQSFREHYKELEQGGGTK"
     gene            4639..>5295
                     /locus_tag="VGT44_17100"
     CDS             4639..>5295
                     /locus_tag="VGT44_17100"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV2582578.1"
                     /translation="MNCEQVEAQLSAYLDNMLSSEERRLVATHLQSCSSCSKILADYS
                     YYDTMIALLPRVSPDAALRERLFASPDLFEALNSDQIPGEAPQEWTLPPITRLPAKHP
                     RRDTPGRPQLIAIPGGRGAHNPDIPDDPHSIHNTQPTPATRQSQQITQPRQQTSRAIR
                     KSGHSLRVMIAALAAALVLALGIGGFFGFTYLSRGLQATGTLGITPPLDISGVTPLSA
                     G"
BASE COUNT         1273 a         1598 c         1330 g         1094 t
ORIGIN      
        1 ggatgtccgc tcgcctgctt attgctactt ttttctcatc aaaatataga cgacttaact
       61 cctactctgt tatactagtt gaggtagcag tgaacagtgg caaccaggcg agcatagtgc
      121 gcactataaa tcatggcaca gaacactgga actcttcacg tcgtagcaac taacgagagg
      181 gaaaaggcat aaaatgagta atccatatga tcctacccag aatgcttacc caccttcagg
      241 accatacaat aacaatgacg ggccttatgc aacgccacaa caaccattta ctggctaccc
      301 acctcaacct gactcgggct acccccagcc gggatatact ccacagcagc cttactatcc
      361 gaccgatcaa ccctattatc caccggccaa cacgccaggc atgtatccgc cgcaacagca
      421 gcaaattggc tacatcggtt atatggccgc tcccgctgag ccgaccaatg gccccggcat
      481 tgccagcctc gtcctgggca ttgtcggcct gttcatttgc tggattccat ttatcggcct
      541 ggccatgccc atcgttgggc ttgtgctggc gtccgtaggc atgaagcgtc tcagcggcaa
      601 aggcctggcg atcgccgggc tggtcctctc catcatagcc ctggtgttgg gaggatgcgt
      661 gtcagccgcc gtcatcactt caattcacta ctaagagcgt tggtctgagg cgatttaacg
      721 ctgaatctgc cccacagtat gccaatcgcc gccatatagc acagggcagt cagcaggaag
      781 ggcagcgtat atccgaagtg cgcgatcaac aggccgccaa gcgaggcagt caaagcccag
      841 ggaacctgga aggcggcctg gtacgcgctg tttgccagcc ctcgatagcg ttcttcgacc
      901 acctccatcg aatagacctg caagacgcca tttgacatat ccataatccc ctgccggaac
      961 ggatagagca acgctgccag tggcacgatt ccaaccaatc ctatcgtcaa cagcaaagga
     1021 atgctcagca gccgcgtcaa cacaatcgca cgaatcctac caacgcgcgc cgccagccag
     1081 ggcgcaacga gtgtcagcag cgcagtcgcc gcattcgccc cgccatcgac cagcccgaac
     1141 agcacagaag aagcatgcaa atgatgcaca aaaaaaatat tcaaatacgg tataaccagc
     1201 cccgccccca gcccggtaat cacataaacc agcgccagaa agaaaaacgg cgtccgcacc
     1261 atcccaccca cccacccggt ggggtgggtc ttatcgatat gtagggacac cccttgtggg
     1321 cgtccggggg aggggtctcc ggaggctgga cggtcggacg ccggtcgtcc gattgatggt
     1381 cgccttgctt cccgcatgag aaacaacggc acaagacttg gtgcggcaat cagaccggca
     1441 aagagcagcg ccagctggta ggagcgcgct tctggttggc cagccagcag cgcgctcgcc
     1501 cacgacggaa atgacgccat cagccagcct tgcgagcgga accagaccgg cagcgcccct
     1561 ccaactactt cgccaacgac cagcgtaatc aagccaagcg agatattcac gctgaacagg
     1621 cctgggcgtt cttcaggtac actattgcgc gttaaaaaag gcgcgttgat caccaccgtg
     1681 aacaccagcg caataccggc aaggaagctt gtcacgagca acggcaccgg ctgtcgaaag
     1741 agtatctgcc cgatacctac cagggcgatc agcagattcg acgcgatcag aatcggtttc
     1801 gcgcgcactc ggtcgatgat gtaaccggct gggaagatag cgataccacc tcctatcgtc
     1861 ccgatgaaca acaccagccc gacaaaatcg gttccataac ccagcgaagt aaggtagaga
     1921 ttgtagagga taagcaaaat gccggtcgtg acgccaccca gcgcagtgtt aatcaggtag
     1981 agcttcgcat ttcgctggaa ccgtccgaac tgccgcgcaa agtcaatgaa gacgttcatt
     2041 ccctatttca acgagctgcc tctcgtcacg agccgccgat gcgggttcaa ccgccagccc
     2101 atcgcctcca gccggcatct caaccacttc gggcagcaag acgcgagcgc gggtagtaag
     2161 gcgtgggcgc tccaccaggc ccggcaagaa aaaagctttg gccatacctt tgaccatcac
     2221 agtgcgcgcg cgtatttcga tcattgtctc tgccacgcga tcagcccttt gttcgacctg
     2281 cgcggagatg gattgcgtcc gctgccgcgc ctgttcgagc ttcttcgaca cctcaaaagc
     2341 ctgcacacgc tgcacagcct gcgccagtcg ctcctgggtt ttgccggtgg gaacgacgct
     2401 ctcaggcgcg gccatcgcct tgttaaccat gtcaacggtc gggcgcagct ttttgatcag
     2461 ttcagccttt tcgcgcaccc aggcgaacaa aaatgcaaag gctgccgtaa gagccaggcc
     2521 gaccaggata ttgacgaaga ggaagatagc aagcacggta gcagcagcct gaccccaggg
     2581 cgcaagcaca tctgcgagcg tgacgctcat gagctgatgc atagctaatc tcctctgctc
     2641 gcggcgttca tcatctccat ctgctccttg cggcgttcct cgtagcgtcg gcgtgtatgg
     2701 cccttgccca cgaagatacg caccgcttcg cgtcctgcca ccaccagggc cgccacgcgc
     2761 acggttggac cgaccgcctg gttggtaagg cgcgccgtcg caccgacggt agaaaccgtc
     2821 tggctggcgc tcctcgccgt atccttcacg gtttcaagcg tgtcctccat cgatcccctg
     2881 accgtgctga ccgtctggtt taccgattcc agcagcggct tcatctcggc gctcaccgtc
     2941 atcactgtgc gaatcaatga cagtaagagg tagacaagca gcgcagtcga aatcaaagag
     3001 aatgcggcaa gcacaatcag tgagatatag aggacatcga gcagcttatc gaaaatgccg
     3061 gccagaataa acccggctac cagtaagatc agcacactga ccgaaacgat cagaagatag
     3121 tttctcgctt ttttcatgtc acgcctccct acaaagacaa agatcattgc tgtgtgcttg
     3181 cattatagta cacaattcaa cacgaaacca gagcaaccca cgtttcgtgt tacttgataa
     3241 tggttgtact tttattaaga ttggctcgtt taagtacagt aggaaacatt ttcctgccgt
     3301 attcatctgt aactctatga gagtaaggag aactctatgc atgcattggc gcttgtcctg
     3361 gccgacggcg taactatcaa agtcggcagc aacacctgga cattcggcac gaacctgatc
     3421 gtctacctca tcattgcggc tatcatcggc ttcctggccg agtttctcgt cggctggcga
     3481 ctgccattcg gcttcgtagg cgcaatcatc gcggctctga tcggcatctg gctgatgacc
     3541 caggtcatcc agttcagcat cagcggtttc aacgatccca ccgttgatgg cgtcccgctc
     3601 gtccacgcct tgatcggcgc gatcattttc gtcgccatct ggcacctgat cacctaccct
     3661 gcctatcgcg gacggcgacg ctattactat cgcagccgat aagctgacct ccaaccatct
     3721 gtagtggcct gtcacagttc aaacgatctg tgggaacgca atgcattgcg ttcccacaga
     3781 tcgtttgaac ctctaatacc tactctcaat gcggcaagta ggaagggtag ctatcgcttc
     3841 ccccaatagt actaactttt ctgtaactga tttcagggaa tctagcaaat atgctaacat
     3901 gattatcagc aaagcacagg agtgccgaaa tcattcattc atctacatgt tactgaaagc
     3961 atgaattgtc cgttccacta cacagaagga aatacgtttt tccgcacccg caggagcaaa
     4021 cagtcgcgcg gcttcggaga tgcacttcta tcacttgtat ggaacgggca agttggtagc
     4081 tatagtcatc gaaggaacgt agcgcatggc cagggcggaa acccaggaaa cttcctggtc
     4141 cttcgaccag atttacgacg aatataaaac accaatttat aattatgtct atcatctggt
     4201 tggggaccgc gaacaagctg atgatcttac gcaggacacc tttttgaaag cgtttcgcgc
     4261 gctgccaaaa atggatggga acctgaaatt gtcggcgtgg ttgtatcgta tcgctacgaa
     4321 cacagcctat gacgcgctgc gtcgccgtaa gctaatcgcc tggctcccct ggcaagaact
     4381 tgaccatgaa cccgcggatg tggaaggggc cgatcctcaa gaaatctatg ggacgaccga
     4441 gttagtacgc gccgctctcc ggcgtatgcc caatcagtac cgggctgcgc tgctgctgta
     4501 cacccaggaa ggtttttcct acagtgaaat cgcgagagca ttaaacatcg ccgaaagcgg
     4561 tgtcaaaatg tatctttcgc gcgcgcgcca gtcgtttcgt gagcattata aagagttaga
     4621 acaaggcgga ggaaccaaat gaactgcgag caagtcgagg cacagctaag cgcttacctt
     4681 gacaatatgc tctcttccga agaacgccgc ctggtcgcca ctcatctcca atcctgctca
     4741 tcatgcagca aaatcctggc cgattatagt tattacgata cgatgattgc gcttttgccg
     4801 cgtgtcagtc ccgatgcagc cctgcgcgaa cgtctctttg cctctcccga cttgttcgag
     4861 gcccttaaca gcgaccagat accgggagaa gccccgcagg agtggacgct cccgcctatt
     4921 acccggctac ccgcgaaaca tccgcgacgc gatactcctg gacgcccgca gctcatcgcc
     4981 attcccggtg gtcgcggcgc tcacaaccct gacatccctg acgaccctca tagcattcac
     5041 aacacccagc cgacgccagc cacgcgacaa tcgcagcaga tcacacagcc tcgccagcag
     5101 acatcgcgag caattcgtaa gagcgggcat agtctgcgtg tgatgatcgc agcgctggcc
     5161 gcagctcttg ttctggcgct cggcatcggc ggcttttttg gcttcacata cctatcgcgt
     5221 gggctacagg cgacaggcac cctgggcatc actccgccac tggacatatc aggtgttaca
     5281 ccgctttcgg ctggc
//
LOCUS       DASZAK010000977         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_274628, whole genome shotgun sequence.
ACCESSION   DASZAK010000977 DASZAK010000000
VERSION     DASZAK010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274628"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VGT44_17105"
     CDS             <1..415
                     /locus_tag="VGT44_17105"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582579.1"
                     /translation="VRVHVCWGNYHGPHHRDVPLKDIIDLLLHIKGHALSIEGANPRH
                     EHEWNVFERVHLPEGKLLIPGVIDSCSNYIEHPEVVAQRIVRYARVVGRENVIAGSDC
                     GFSTFVGASAVAPSIAWAKLRSLAEGARLASEELW"
     gene            complement(528..1814)
                     /locus_tag="VGT44_17110"
     CDS             complement(528..1814)
                     /locus_tag="VGT44_17110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2582580.1"
                     /translation="MTRPAEADAQPQGTLWLLDLDPPLPVGPMPQVAVVFMRAGPEVA
                     QELAQAMHLDDHTLVLQRFANGRQCYVGRVDGRMVTYGWVTFDEEEIGELSLNIRLKA
                     GEAYIWNCATLPAYRGQRLYPALLAYIVGELHRQGLRRIWIGTDTDNLPSQSGIAQAG
                     FRPIGDIVSSHGSTMRRSWLRGRHGIPAPLVEDMRSALLGDGKEVWLDANSNDQSAPG
                     VEPHQGQPLVLAGDPPESARAAMIMIHGRGASARDILTLVPDLQCPGFAYFAPQAAGN
                     AWYPNSFLMPIASNEPGLSSGLAVISSLLNQLARLGIPAERTILLGFSQGACLMLEYV
                     ARNARRYGGVAGLSGGLIGPEGTPRNYPGSLADTPVFLGCSDADPHIPKQRVEHTAEI
                     LQRLGGNVTTRLYARMGHMVNEDELRFVQGMMAAIK"
     gene            complement(1811..>2335)
                     /locus_tag="VGT44_17115"
     CDS             complement(1811..>2335)
                     /locus_tag="VGT44_17115"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2582581.1"
                     /translation="GSNRETTIDFYQGVLGMPLVMEQPNLDVPEETHLYFDAGDGRLL
                     TFFVRSTRVNDSTPNPEGIGNLHHLALTVSHATYTQVAQRLNERGIWNTGNIDRGFMD
                     SIYFRDPNGQLLEMACYKFVPPEGCSVVDVLTTAHRLRVAEGAYNIQDSHLADAIAEL
                     SRRKVTPITEVALT"
BASE COUNT          464 a          695 c          723 g          453 t
ORIGIN      
        1 ggtgcgcgtc cacgtgtgct ggggcaacta tcacggcccg catcaccggg atgtgccgct
       61 caaagacatc atcgacctgc tcctgcacat caagggccat gcgctctcca tcgaaggcgc
      121 caacccgcgc catgaacacg agtggaacgt cttcgagcgg gtacatctgc cggagggaaa
      181 gctgctgatc cctggggtga tcgactcctg ctccaactat atcgaacatc cagaggttgt
      241 ggctcaacgc atcgtgcgct atgctcgagt cgttggcaga gaaaacgtca tagccggttc
      301 ggactgtggt ttcagcacct tcgtaggtgc gagcgcggtc gcgcccagta tcgcctgggc
      361 caaactgcgc tccctggccg aaggcgcccg gctcgcttct gaagaactat ggtaatgtga
      421 atatccagag ggcgcccgca aggggacgcc cctacaatga tccgacgaac tgctgaatca
      481 cttcgtagtc attgtagggg gtccccttgc gggcgccctt tcggaaatta tttgatcgca
      541 gccatcatcc cctgcacgaa gcgcaactca tcctcgttga ccatgtggcc catgcgcgcg
      601 tagagccgcg tggtcacgtt tccacccagg cgctgtaaga tctcggcggt atgctcaacg
      661 cgctgttttg ggatatgcgg atcggcgtcg ctgcatccta gaaaaacggg cgtgtccgcc
      721 agcgagccag ggtagttgcg cggcgtcccc tccggtccga tcaggccgcc gctcaggccg
      781 gcgacgccac cgtagcgccg ggcattgcgc gcgacatatt cgagcatcag gcaagcgccc
      841 tgcgagaaac ccagcaggat tgtgcgttcg gcagggatgc ccaaccgggc taactgattg
      901 agcagcgatg aaatcacggc cagccctgaa gagaggcccg gttcgttgct ggcaatgggc
      961 atgagaaagc tattgggata ccaggcattt ccggcggctt gcggcgcgaa ataggcaaaa
     1021 ccgggacatt gcaggtctgg aaccagcgtc aggatatctc gcgccgatgc tccgcgccca
     1081 tgtatcataa tcatggccgc gcgtgcgctc tcaggtgggt cgccagcgag tacgagcggc
     1141 tgcccctggt gtggttctac gccaggggct gattggtcat tggagtttgc gtccaaccag
     1201 acctccttgc catcgccaag cagcgcagag cgcatgtcct ccacgagcgg cgcgggaatg
     1261 ccatggcgtc cgcgcagcca cgagcggcgc atggtcgagc catgactgct gacgatatcg
     1321 ccgattggcc ggaagcccgc ctgggcgatg ccgctttggg agggcaggtt gtccgtgtcc
     1381 gtgccgatcc agatgcggcg cagaccctgg cgatggagtt cgccgacaat gtaggccagc
     1441 agcgcaggat agagacgctg cccgcgatat gcaggcagcg tagcgcagtt ccatatgtag
     1501 gcttccccgg ccttgagtcg aatgttgagg ctcagctcgc caatctcttc ttcatcgaaa
     1561 gtgacccagc catacgtgac cattctgcca tcgactcgac cgacgtaaca ttgccgccca
     1621 ttggcgaagc gttgcagcac cagcgtatga tcgtcgagat gcatagcctg cgccagttcc
     1681 tgcgctactt ctggcccggc gcgcatgaag accaccgcaa cctgtggcat agggccaacc
     1741 ggcaggggcg ggtcgagatc cagcagccag agcgtaccct ggggctgcgc atcggcctcg
     1801 gcaggtcgcg tcatgtcaaa gccacctctg tgatgggcgt gactttacgt cgggacagtt
     1861 cggcaatggc gtcggccagg tgagaatcct ggatattata ggcaccctca gctacccgca
     1921 gccggtgcgc ggtagtgagg acatcgacaa cactgcatcc ttctggagga acgaatttgt
     1981 aacaggccat ctcgagcaac tggccattgg ggtcccggaa gtagatcgag tccatgaagc
     2041 ccctgtctat gttgcccgta ttccagatgc cacgctcgtt gaggcgctga gcgacctggg
     2101 tgtaggtcgc gtgtgagacg gttagagcga ggtgatgcag gttgccgatg ccttcgggat
     2161 tgggagtgga atcattcacc cgcgtggagc gcacgaagaa ggtaagcaac cgaccatcgc
     2221 ccgcatcgaa gtagaggtgc gtctcttccg ggacatccag attgggttgt tccatcacca
     2281 gtggcattcc cagcactccc tggtagaaat cgatagtcgt ctcacggttg gaacc
//
LOCUS       DASZAK010000978         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_381491, whole genome shotgun sequence.
ACCESSION   DASZAK010000978 DASZAK010000000
VERSION     DASZAK010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381491"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..1922
                     /locus_tag="VGT44_17120"
     CDS             99..1922
                     /locus_tag="VGT44_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2582582.1"
                     /translation="MGFIMEGLDAEAYDRAYTDRQLVRRIIGYFRPKARIMLIVAGMI
                     VLASILDTLAPVLISSGIDLVVASPALRTILLLVGVILLSGVLSWFCNFFRQWLTARC
                     VGDVVLSLRRDAFHAVMARDMSFFDEFQSGKIVSRVTSDTEDFATVVTLALNLLSQVL
                     IFFLIAGVIVVKSVQLALLTFTIVPAIVLIAIIFRILARRATRRTQRSMARVNANVQE
                     VISGITVAKNFRQEQNMYDEFKEVNKQSYHVNVRSGLIYDGVFPLLVMVANIGMVIVV
                     YFGGLDVFSRSITAGDWYLFVQALRILWFPLTSIASFWSQFQLGLAASERVFALIDAE
                     PRVLQHDHQLVPHLDGAIELKDIFFSYDDRQVVLAGLNLSIAAGQTIAIVGHTGAGKT
                     SIARLVARFYEFQGGQILIDGKDIRSFDLRDYHRRLGIVPQQPFLFSGTIADNIRYAR
                     QDASDEEVASAAQHIGGGDWLDALPDGLETLVGERGRSLSLGQRQLVALARVLLQDPS
                     IIILDEATASVDPLTEAQIQEGLDLILQHRTSILIAHRLSTIRHADRIIVLDRGAIVE
                     EGSHDTLLRQGGHYAQLYNTYWRHQSPDYRPGEGFVRVANG"
     gene            complement(1969..2334)
                     /locus_tag="VGT44_17125"
     CDS             complement(1969..2334)
                     /locus_tag="VGT44_17125"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEV2582583.1"
                     /translation="MATRDELREALDDYVMNASTNERVQRSLKSWNCVIYIEATDVDA
                     SFTMTIAGGKGSISDGSTTQPDLIVRGNSEDLANIFWCDENPASNYMQGAIKVQGSPD
                     NVMRLDAMALLVFLEGQKG"
     gene            complement(2501..>3585)
                     /locus_tag="VGT44_17130"
     CDS             complement(2501..>3585)
                     /locus_tag="VGT44_17130"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2582584.1"
                     /translation="YKRGQRTTYAGTIPTSEPLVTMEVVEMLLNLSMMASEVPLDAPW
                     TAPLATEWDAQTMATWAELNVQNEGARDLLTLVVQAVFSVEPRDISLLPFLFYVHSAG
                     NLNMLLSVAGGAQESRFVGGAQSIANSVAEALGERVILNTPVHTIVQDETGVRVESDA
                     LSVTARRAIIAIPPTLAGRLRYRPALPGYRDQLTQRVPMGTVIKVHCLYETPFWRDEG
                     LSGQVVSDAGIVRITYDNSPENSRPGVLLAFIEGDEGRIWGRRSVEERRAAVLECLAR
                     YFGDKARHPFEYLEQNWAEEEYTRGCYAGVMPPGVWTEYGEALRAPVGRLHWAGTETA
                     TVWNGYMDGAVRSGERAAAEVLAALT"
BASE COUNT          684 a         1258 c          911 g          732 t
ORIGIN      
        1 ttggaggtca cgatccactc gccacccgga caggcacaag gcccccactc cgccgctctg
       61 ccgccccctg tccctacaca aaatgcaaag gagcatatat gggcttcatt atggaaggtc
      121 ttgacgcgga agcatatgac cgtgcctata ccgacaggca gcttgtcagg cgcatcatcg
      181 gctattttcg gccaaaggcg cgcatcatgc tgatcgtagc gggcatgatt gtcctcgcct
      241 ctatactcga cacgctcgcg cccgtcctga tttcgagtgg catcgacctg gtggtggcct
      301 cgcccgccct gcgcaccatt ctgctgctcg tgggggtcat cctgctctct ggcgtgctat
      361 cgtggttttg caacttcttt cgccagtggc tgaccgcccg ctgcgtcggc gatgtcgtgc
      421 tgagcctgcg ccgcgatgcc ttccacgcag tgatggcacg cgacatgtcc ttcttcgatg
      481 agttccagtc gggcaagatc gtctcgcgcg tgacttccga taccgaggat ttcgcgacgg
      541 tcgtgaccct cgccctcaac ctgctcagcc aggtcttgat cttcttcctt atcgcgggcg
      601 tgatcgtcgt caaaagcgtc cagcttgctc tactgacctt caccatcgtg cctgccatcg
      661 tgctgatcgc gatcatcttc cgcatcctcg cgcggcgcgc cacgcggcgt acccagcgtt
      721 cgatggcgcg tgtcaacgcc aatgtgcaag aggtcatcag tggtatcact gtcgccaaga
      781 actttcgcca ggaacagaac atgtacgacg aatttaagga ggtcaataag cagtcgtatc
      841 atgtcaatgt gcgttccggc ctgatctacg acggcgtctt ccctctgctg gtcatggtcg
      901 ccaacatcgg catggtcatc gtcgtctact tcggcggact ggatgtcttc agccgcagca
      961 tcacagcagg cgactggtat ctcttcgtgc aagcccttcg catactctgg ttccctctca
     1021 ccagcatcgc ctcgttctgg agtcagttcc agctcggcct ggccgccagc gagcgcgtct
     1081 tcgccctgat cgacgccgag ccgcgtgtgc tacagcatga ccaccagctc gtcccacacc
     1141 tggatggcgc tatcgaactg aaagacatct tcttcagcta tgacgaccgc caggtagtgt
     1201 tggccggact caatctctcc atcgcagcag gccagaccat cgccatcgtg ggacataccg
     1261 gcgcgggcaa aaccagcatc gcccgcctgg tagcgcgctt ctacgaattc cagggcggcc
     1321 agattttgat cgacggcaaa gacatccgca gctttgacct gcgcgactac caccgccgtc
     1381 tgggcatcgt gccgcagcaa ccgttcctct tctccggcac gatcgccgac aatattcgct
     1441 acgcccgaca ggacgccagc gacgaagaag ttgcctctgc cgcccagcac atcggcggcg
     1501 gcgactggct agatgccctg cctgacggcc tggagacgct ggtcggcgaa aggggtagat
     1561 cgctctcact cggccagcgc cagctcgtcg cgctcgcccg cgtcctgctg caagacccct
     1621 ccatcatcat cctcgacgag gccaccgcca gcgttgatcc actcaccgaa gcccagattc
     1681 aagaggggct ggacctcatc ctgcaacatc gcacctccat cctgatcgcc caccgcctct
     1741 ccaccatccg ccacgccgac cgcatcatcg tgctggatcg cggcgccatc gtcgaagagg
     1801 gcagccacga caccctcctc cgccagggcg gccactacgc gcagctctac aacacctact
     1861 ggcgtcacca atccccggat tatagaccag gggaaggatt tgtacgggtt gcaaatgggt
     1921 aatggtgtgg atgccaagcg accgcgaggg tcgccctggc agcctcgctt agcccttctg
     1981 tccctccaaa aataccaaca acgccatcgc gtccaaccgc atcacattat ccggcgaccc
     2041 ctgtaccttg attgcccctt gcatatagtt cgaggccgga ttctcgtcgc accagaagat
     2101 gttggccagg tcttcgctat tgccgcgcac gatcaggtcg ggctgcgtcg ttgagccatc
     2161 gctaatgctg cctttgccgc ccgcgatagt catagtaaac gaggcatcta catccgtcgc
     2221 ctcaatataa atcacgcagt tccagctttt cagcgaacgc tgcacgcgct cgttcgtgct
     2281 ggcattcatc acataatcgt ccagcgcctc gcgcaattca tctcgtgtcg ccataaagtt
     2341 actcccgatc atagctattg taatgtaggt acgagcctgt catcttgagc gcagcgaagg
     2401 atctcggtcc tggcgcgtca gatccttcgc tgcgctcagg atgacaggct cggttgtggc
     2461 tggtaaattt cattaccggc gtatgcgtgg aaaaccggca ttatgttaac gctgccagca
     2521 cctccgccgc cgcgcgttcg cctgaacgca ccgcgccatc catatagccg ttccacaccg
     2581 tcgccgtctc cgtcccggcc cagtgcaaac gacccaccgg cgcgcgcagc gcctcgccat
     2641 actccgtcca cacgcccggc ggcatcaccc cggcgtagca gccgcgcgta tactcctcct
     2701 ccgcccagtt ctgctcaaga tattcaaatg gatgtctcgc cttgtcgcca aaatagcgcg
     2761 ccaggcattc cagcacggcg gcacggcgct cctccacgct tctacgcccc cagatgcgcc
     2821 cctcgtcgcc ctcgataaat gccagcaaca cgcccggtct gctattttcc ggcgaattat
     2881 cataggtgat gcgcacaatc cccgcatcgc ttaccacctg ccccgacagc ccctcatcac
     2941 gccagaacgg tgtctcgtac aggcaatgca ccttgataac cgtccccatt ggcacgcgct
     3001 gcgtcaactg gtcgcgatag cccggcagcg ccggtcggta gcgcagtcgc cccgccagcg
     3061 tcggcggaat agcaatgata gcccgccgcg ccgtcacgct aagcgcatca ctctccactc
     3121 gcacacccgt ctcatcctgc acaatagtat gcaccggcgt gttgagtatc acccgctcac
     3181 ccaacgcttc agcgacactg ttcgcgatgg attgcgcacc tcctacaaag cggctctcct
     3241 gtgcgcctcc cgccacgctc aacagcatat tcaaattccc ggcggaatgc acataaaaga
     3301 gaaagggcag cagcgaaata tcgcgcggct caaccgagaa gaccgcctgc accaccagcg
     3361 tcaacaggtc gcgcgccccc tcgttctgca cattgagttc agcccacgtc gccatcgttt
     3421 gcgcgtccca ttccgtcgcc aggggagccg tccagggcgc atcaagcggc acctcgctcg
     3481 ccatcatgct caagttcagc agcatctcca ccacctccat cgtcaccagc ggctcgctcg
     3541 tcggtatcgt tccagcatac gtagtacgct gcccgcgctt atact
//
LOCUS       DASZAK010000979         2576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_59965, whole genome shotgun sequence.
ACCESSION   DASZAK010000979 DASZAK010000000
VERSION     DASZAK010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59965"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VGT44_17135"
     CDS             <1..1111
                     /locus_tag="VGT44_17135"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEV2582585.1"
                     /translation="GRLRPMSAYTMGLIFRWARLASRVPVLANIVTQAPLISSLAKAI
                     IAVAPQRRLPVFASPTFKEWLRRRGPRNVDAPRVILWPDTFNNYLHPAPLQAAVEVLE
                     AAGYQVVVPQQALCCGRPLYDFGMLDTAKRLLRQVMNALEPEINAGTPIVGLEPSCVA
                     VFRDELTNLFPHDERARRLKEQTFILSEFLEKKATHFQLPTLQHRAVVHMHCHHKAIL
                     RTGSEEAVLRKLGLDYKLLDSGCCGMAGSFGFEKDHYEVSIASGERVLLPAVREADQD
                     TLIITDGFSCREQIAQTTDRRALHLAEIIQLALHQDAQDTQASPAIVDPPCAAPVSPL
                     KTGMALVGGIGLACLSALLWMRGKRKASRPGHDRL"
     gene            complement(1157..1729)
                     /locus_tag="VGT44_17140"
     CDS             complement(1157..1729)
                     /locus_tag="VGT44_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV2582586.1"
                     /translation="MRKIITTTWVTLDGFIAGPNGEMDWIGEIYDEAMSTYENNLLNE
                     ADTLLLGRVTYQSFAGSWPYVPDSPNASEYEKEYAHKLNAMRKIVFSRTLDGVEWNNS
                     RLVKEVVPEEIEQLKHEPGHDMLIYGSASLVRTLTNLGLIDEYQILVHPVILGGGKPL
                     FQDIKDKVKLNLVNTRAHPSGVVVLTYSKV"
     gene            complement(1778..2488)
                     /locus_tag="VGT44_17145"
     CDS             complement(1778..2488)
                     /locus_tag="VGT44_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV2582587.1"
                     /translation="MIFFDEDRPSDSPFVDRVWRCHSEGAAPFLSIAASRCELVVTRL
                     QGKVTVTVRGPETRATPLGDCPGDGEWLGLLLKLGAFLPDLPTGTLVDAEVNMPTVSK
                     DSFWLFGSAWQLPDYDNADTFVDWLARKGLLAREPVVDAAMRLKDRSLSTIQRRFLRA
                     TGLTQSAARQIERARSATHLLQQGVSILDTVEQAGYFDQPHLTRSLKYLIGQTPAQIL
                     QKSRPEQMPFLYKTTPFE"
BASE COUNT          517 a          801 c          716 g          542 t
ORIGIN      
        1 gggcaggctg cgtcccatga gcgcctacac gatgggcctc atcttccgct gggcgcgtct
       61 cgctagtcgc gtgccagttc tcgccaatat cgtcacccag gcgccgctaa tcagctcgct
      121 cgccaaagcc atcatagccg tcgcgccgca gcgccgcctg ccggtcttcg cctcgcccac
      181 ctttaaggag tggttgcgca ggcgcggccc gcgcaatgtg gacgcgccca gggtcatcct
      241 gtggcccgat accttcaaca actacctgca ccctgcgcca ctgcaagcag ccgtcgaggt
      301 gcttgaagcc gctggctacc aggttgttgt ccctcaacag gcgctgtgct gcgggcgtcc
      361 gctctacgat ttcggcatgc tcgacaccgc caaacgcctc ctgcgccagg tcatgaacgc
      421 gctggagcca gaaatcaacg cgggtacgcc catcgtcggg cttgaaccga gctgcgtggc
      481 cgtcttccgc gacgaactga cgaatctgtt cccgcatgat gagcgcgcca ggcgcttaaa
      541 agagcagacc ttcattctca gcgagttcct tgagaagaag gccacgcact tccaactccc
      601 gacgttgcag cacagggccg ttgtccacat gcactgccat cacaaagcta ttctgcgcac
      661 agggtcggag gaggctgtac tcaggaagct cggactggac tacaagctac tcgactccgg
      721 gtgctgtggc atggccggct ccttcggctt cgagaaggac cactacgagg tctccatcgc
      781 cagcggcgag cgtgtcctgc tgcccgctgt gcgcgaggcc gaccaggata cgctgattat
      841 caccgacggc ttcagttgcc gcgaacagat cgcgcagacc accgaccgcc gcgccctgca
      901 cctggccgag atcatccaac tcgctcttca ccaggacgcg caagacacgc aggcttctcc
      961 cgcaatcgtg gacccgccgt gcgccgcacc cgtctcgcca ttgaaaaccg gcatggcgct
     1021 tgtgggtggc atcggcctgg cctgtctgag cgccttgcta tggatgcgag gcaagaggaa
     1081 agcaagccga cctggacatg atcgactcta gcttgccagg gcgatggcca gctctctggt
     1141 cacagaatcc tgggatttac accttcgaat aggtaaggac caccactcca gaaggatgcg
     1201 ccctggtgtt gaccaggttc agcttcacct tgtctttgat gtcttgaaag agaggcttgc
     1261 cgccgcctaa aatcaccgga tggactaaga tctgatattc atcgatcaag ccaagattgg
     1321 taagagttcg caccaggctg gcgctcccat aaatcagcat gtcgtgtcca ggctcgtgtt
     1381 tcagttgctc gatctcttct ggaacgacct ctttcaccag ccgtgaattg ttccactcca
     1441 cgccatccag cgtcctggaa aacactattt tgcgcatcgc attcaacttg tgagcgtatt
     1501 ctttctcgta ttctgaggca ttaggactgt cagggacata gggccaggag ccagcaaaac
     1561 tttgatacgt cacacgtccg agcaagagcg tatcggcctc gttgagcaga ttgttctcat
     1621 aagtactcat ggcttcgtcg tagatctccc cgatccaatc catttctcca tttggtccag
     1681 cgatgaagcc gtcaagagtg acccacgtgg tggtaataat ttttctcatg ccattctcct
     1741 tatccttttg tcgtttatcg gacggagcta ttatcactca ttcaaagggc gtcgtcttgt
     1801 ataaaaacgg catctgctcg ggtcggcttt tctgaaggat ctgggcaggc gtttgtccga
     1861 tgaggtactt cagcgatcgg gtcaggtgcg gctgatcgaa atagcccgcc tgctcaacgg
     1921 tatccaggat cgagacgccc tgctgtaaga ggtgcgtggc ggagcgggcg cgttcgattt
     1981 gacgagccgc gctctgcgtc agcccggtcg cccgcaagaa gcgacgctgg atagtactca
     2041 gtgagcggtc tttcaggcgc atggcggcat ctacgaccgg ctcgcgcgcc agcaggccct
     2101 tacgcgccag ccagtcaaca aacgtgtcgg cattgtcata gtcaggaagt tgccaggcag
     2161 agccaaagag ccagaaggag tccttcgaaa ctgtgggcat gttcacctct gcatccacca
     2221 gcgtcccggt gggcagatcg ggcagaaacg cgcccagctt caagaggagg ccaagccact
     2281 ccccgtcccc agggcaatct ccaagcggtg tggctctggt ctctggcccg cgcaccgtca
     2341 cagtgacttt gccctgaagt ctggtcacca ccagctcgca gcggctcgcg gcaatggaga
     2401 gaaaaggggc cgcgccctcg ctatggcagc gccagaccct gtcgacaaag ggtgagtcgg
     2461 atggtctgtc ctcgtcaaag aaaatcattt cacacctcgc tttcgttgct gtgcagcggt
     2521 ttggcaaacg cgccgttcat actgtgttcc aggcctcacc tgctcccatg atagca
//
LOCUS       DASZAK010000980         5369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_167334, whole genome shotgun sequence.
ACCESSION   DASZAK010000980 DASZAK010000000
VERSION     DASZAK010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167334"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VGT44_17150"
     CDS             <1..1122
                     /locus_tag="VGT44_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582588.1"
                     /translation="RDVVEQYYSQLETASAMGQTVFVYVLLLPAFLFLLLFPFVLSGL
                     LTNYYFHIHPYNIFNPTNDLSDTAYWIDSILSSIVSLLIFFLFIRYSSKFIDKTSNIG
                     RSIFLLFFFLSLPFLLMIATSVFLSRYYFWFLSDSSNLLNYFLASILIDFIFLVTCAL
                     IIVISGISGFLILNMIPEKRYPMAVVVDSLLTILRDLPADVRATDQRRQLYLLEKAAR
                     HLEHYLCKKVPSGDTATDMWTRENAHQIAAALRDKKKWIITPKPDTNSCLVLSIGTTF
                     NHVITGNLDALERMQPEKFSHPRSWLTRIIKIIRTLFIAFLPPAALWAIQLTPLAGLI
                     APARTYLLFGVLAWAVVTILRELDPDFSQKISTIRDMLR"
     gene            1256..3808
                     /gene="glgP"
                     /locus_tag="VGT44_17155"
     CDS             1256..3808
                     /gene="glgP"
                     /locus_tag="VGT44_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEV2582589.1"
                     /translation="MKVYGRVTVFPIMPPRISRLYELAYNLWWSWHPEALDLYRSLDS
                     ELWDAAGHSPVRFLSEVKPVLLEVAAEDAAYLERYDSVMRDFDRYMHPGPDETWFERE
                     FPEYIDQVIAYFSAEFGLHEALPIYSGGLGILSGDHCKEASDLGLPFVGVGFLYPQGY
                     FRQHITRDGAQEAFYDKLHFSEAPATPALGPDGNEVMIQVDLPGRRIHAKVWKVQVGR
                     IPLYLMDTDVPPNAPNDRELSARLYGGDREMRISQEIVLGIGGVRALRALGIDPAVWH
                     INEGHAAFMGLERCRELVVLGLSFNEAREAVAANSLFTTHTPVPAGNDTFSYDLVDKY
                     FGSYWGQLGLSRDQFMDVAREDHGWGPTYGMTVLALRLTGHHNGVSKLHGDVSRAMWQ
                     FLWPGVDPDEVPIESITNGIHSPSWIAPTMNALFKRYLGEDWSERVDDPALWSKIDTI
                     PDEGLWQVHVQCKQALIDYARQRLHGHHLRLGEGTTQMAEFERMLDPNALTIGFARRF
                     ATYKRATLIFRDVERLKRILNDPKRPVQIVFAGKSHPADGPGKALIEQVYRISRSDAF
                     RGKIVFLENYDINMARYLVSGADLWLNNPIRPHEASGTSGQKASLNGLPNCSILDGWW
                     AEGYNGQNGWAIGEEREYHDSEAQDEADSLSLYQALEESIIPLYYDRDANGIPHGWIG
                     VMREAIRTCAPMFSMRRMVKEYTTRFYVPEMQQGLRIEQSGFDQARVLASWKERVRQA
                     WSGMEIYAEGQRDGQLSLGEGIDMRAWVRAGDLRPDDLTVELVYGEASEDHAVAQHAL
                     PMAYIKKELDGSLRYELRLQPPESGSIAYGVRVLPNHPALANKHELGLIRWG"
     gene            complement(3865..4551)
                     /locus_tag="VGT44_17160"
     CDS             complement(3865..4551)
                     /locus_tag="VGT44_17160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEV2582590.1"
                     /translation="MKYSSWFVVIAAIFVTCLITANIIAVKLILFLGFLVPAGIIVFP
                     LSYLFGDILTEVYGYSAARRVIWLGFACNLLAVIAIYIGGLAPAAPFWQGQAAYNTIL
                     GFTPRLLLASFSAYLVGEFTNSFVLARLKIATHGRWLWTRTIGSTLIGEGLDTLIFIT
                     IAFWGIIPSSSMLQAILTQWIFKVVYEIVATPFTYAVVGFLKRKEGVDTYDYHTNFSP
                     ILFRRAASER"
     gene            complement(4570..>5369)
                     /gene="queC"
                     /locus_tag="VGT44_17165"
     CDS             complement(4570..>5369)
                     /gene="queC"
                     /locus_tag="VGT44_17165"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEV2582591.1"
                     /translation="PRTGWGFFMPYLDVYSFVRIFTRCSTRKEQGAIMQHKAVVLLSG
                     GLDSTTTLAMARQQGFELLALSFEYGQRHQREVEAAAAVARYYGVTRQQTIAIDLRAF
                     GGSALTADIPVPHRRSLSEMATDIPITYVPARNTVFLSFALAYAEVTGANDIFLGINA
                     IDYSGYPDCRPEYLQAYEDMANLATKASTQDGRRFHLHAPLMRLGKADIIRKGVELAV
                     PYHLTWSCYEGGELACGTCDSCLLRLNAFAEVGIADPIGYAADGITL"
BASE COUNT         1184 a         1521 c         1506 g         1158 t
ORIGIN      
        1 agagatgtcg tggagcaata ttattctcaa ttggagacag catctgccat gggccagaca
       61 gttttcgtct acgtattact ccttccagct ttccttttcc tacttttatt tcctttcgta
      121 ctttcaggtc tattaacaaa ctattacttc catattcatc catataatat tttcaatcct
      181 acaaatgacc tatcagatac agcttactgg attgatagta tactatcttc catcgtatct
      241 ctgctcattt ttttcctttt tatcagatat agctctaaat ttatagacaa aacctccaat
      301 atcggccgct ccatcttcct gctattcttc ttcttatccc ttccatttct tttgatgatt
      361 gcgacttccg tttttttatc tcgatactat ttttggtttc tcagtgactc atccaatctt
      421 ctcaattatt ttcttgcatc cattcttata gatttcatat ttcttgttac ttgcgcctta
      481 attattgtga taagcgggat atcaggattc ttaatcctta atatgatacc ggaaaaacgt
      541 tatcccatgg ccgtagtagt cgattccttg cttactattt tgagggattt accggctgat
      601 gtaagggcta ctgaccaacg acgccagctc tatttactag aaaaggcagc caggcattta
      661 gagcactatc tctgcaagaa agtacccagc ggggatactg ccacagacat gtggacacga
      721 gaaaacgctc accagatagc agccgccctg cgtgataaaa agaagtggat tatcacaccc
      781 aaaccagata caaactcctg tctagtcttg agcattggta caacgttcaa tcatgtcata
      841 actggcaact tggacgcgct agagcgtatg cagcctgaga aattctcgca tcctcgttcc
      901 tggctgactc ggataatcaa gatcataaga acgctcttta tagcgtttct tccaccagca
      961 gctttatggg cgatacaact gactccttta gcgggtttga tcgcgcctgc tcgaacgtat
     1021 ttactcttcg gcgtattggc atgggcagta gtaacgatac ttagggaact tgatccagat
     1081 ttcagccaga aaatatcgac tatcagagat atgttacgct gataacaggc caaatatcac
     1141 cgagaaacta ttgctacaac tcctctttac tcataaccca cctcgtgcat ttgaccgcat
     1201 ttcgcccaca agatatactt aacagtagat gtcgttgaga aaggaatctt cttctgtgaa
     1261 agtgtatggt cgtgtaacgg tcttccccat tatgcccccg cgtatcagcc gcctgtacga
     1321 actggcctat aacctgtggt ggagctggca tccggaggcg cttgacctgt atcgttccct
     1381 cgattccgag ctgtgggacg ccgccggtca tagcccggtg cgctttctca gcgaggtgaa
     1441 accggtatta ttagaagtgg ctgccgagga cgccgcgtat ctagaacgct acgatagcgt
     1501 gatgcgcgac ttcgaccgct atatgcatcc cggccccgat gagacctggt ttgagcgcga
     1561 attccccgaa tatatcgacc aggtgatcgc ctatttctcg gcggaatttg gcctgcacga
     1621 ggcgctgccg atctattcgg gcggcctggg catcctatcg ggcgaccatt gcaaggaggc
     1681 aagcgacctg gggctgccct tcgtgggtgt cggcttcctc tatccccagg gctatttccg
     1741 tcagcatatc actcgcgatg gagcgcagga ggcgttttac gataagctgc acttctccga
     1801 agcgcccgcc acgcccgcgc ttgggccgga cggcaacgag gtgatgatcc aggtcgatct
     1861 gccgggccgc cgcatccacg ccaaggtctg gaaggtgcag gtcggacgca tcccgctcta
     1921 tttgatggat accgatgtgc cgccgaacgc gccaaacgac cgcgagctgt cggcgcgcct
     1981 gtatggtggc gaccgcgaaa tgcgcatttc acaggagatc gtactgggta tcggcggcgt
     2041 gcgcgccctg cgggcgctgg gcattgaccc ggcggtctgg catatcaacg agggtcacgc
     2101 ggcctttatg ggactggaac gctgccgcga actggtcgtg ctggggctga gcttcaacga
     2161 ggcgcgcgag gcagtcgcgg ccaattcact cttcaccacg cacacgcccg tgccagccgg
     2221 gaacgatacc ttttcgtatg acctggtgga taaatatttc ggctcgtatt gggggcaact
     2281 tggcctctcg cgcgatcagt tcatggacgt agcgcgtgaa gatcacggct ggggtcccac
     2341 ctacggcatg acggtgctgg ccctgcgcct gacggggcat cataacggcg tgagcaaact
     2401 gcacggcgac gtctcgcgcg ccatgtggca attcctctgg ccgggcgtcg accctgacga
     2461 ggtgccgatc gagtcgatca ccaacggcat ccactcgcct tcctggatcg cgcccaccat
     2521 gaacgcgctc ttcaaacgct acctgggcga ggactggagc gagcgcgtcg atgatcccgc
     2581 tctctggagc aaaatcgata ccatccccga cgaggggttg tggcaggttc acgtgcaatg
     2641 caagcaggcg ctgattgatt acgcgcgcca gcgtctgcat gggcatcacc tgcgcctggg
     2701 cgagggcaca acgcaaatgg cagaatttga gcggatgctc gaccccaatg cgctgacaat
     2761 tggcttcgcg cgccgcttcg cgacctacaa acgcgccacg ttgattttcc gcgacgtgga
     2821 acgcctcaag cgcatattga acgatcccaa acgtcctgtg cagatcgtct tcgccggcaa
     2881 gtcgcacccc gccgatgggc cgggcaaagc gctgatcgag caggtctatc gcatctcgcg
     2941 cagcgatgcc ttccgaggga agatcgtctt cctggaaaat tacgatatca acatggcgcg
     3001 ctacctcgtt tcgggcgcgg atctctggct gaacaatccg attcgtccgc acgaggccag
     3061 cggtacgagc gggcagaaag cctcgctcaa cggcctgccg aattgcagca tcctggacgg
     3121 ctggtgggcc gagggctaca acggacaaaa cggttgggcc attggcgaag aacgcgagta
     3181 ccacgattcc gaggcgcaag atgaagccga cagcctttcg ctctaccagg cgctcgaaga
     3241 gtccatcatc ccgctctact acgaccgtga cgcgaacggc atcccgcacg gctggattgg
     3301 cgtcatgcgc gaagccatcc gcacctgcgc ccccatgttc agtatgcggc gcatggtcaa
     3361 ggagtacaca acgcgcttct acgtgcccga gatgcagcag ggcttgcgaa tcgagcagag
     3421 cggcttcgac caggcgcgtg tcctggcatc ctggaaggag cgcgtacggc aggcgtggtc
     3481 aggcatggag atctatgctg aggggcagcg cgacgggcag ctgagcctgg gcgaaggcat
     3541 cgacatgcgc gcctgggtgc gagcaggcga cctgcgcccc gacgacctga ccgtcgaact
     3601 ggtctacggc gaagccagcg aagaccacgc cgtcgcgcaa cacgccctgc caatggccta
     3661 catcaagaag gaactcgacg gctccctgcg ctacgaactg cgcttgcagc cgcccgaaag
     3721 cggcagcatc gcctacggtg tgcgcgtgct gcccaaccat cccgccctgg cgaacaagca
     3781 cgaattagga ttgattcgct ggggctaaga cagtcgccta gcatagtaca aggagggcga
     3841 ccgcaagggt ctccctcgct tcccctatcg ttcagacgca gcccgtcgga acagaatcgg
     3901 gctgaaattg gtgtggtaat catacgtatc gacgccctct ttacgcttga gaaacccgac
     3961 gacggcgtag gtgaagggcg tggcgacgat ttcgtagacg accttgaaaa tccactgtgt
     4021 caggatagcc tggagcatgg aggaggacgg gatgatgccc cagaaggcga tggtgatgaa
     4081 gatgagcgta tccaggccct caccgatcag cgtcgagccg atggtgcgcg tccagagcca
     4141 gcgcccatgc gtggcaatct tgaggcgcgc cagcacaaaa gagttggtga attcccccac
     4201 caggtaagcg ctaaaggaag ccagcagcag gcgcggcgtg aagccgagga tggtattgta
     4261 ggccgcctgt ccctgccaga agggcgcggc gggcgcgagt ccaccgatgt agatggcaat
     4321 gacagccagc agattgcagg caaaccccag ccagatgacg cgccgcgccg cgctgtagcc
     4381 atacacctcg gtgagtatat ccccaaatag gtaactgagc gggaagacga taattccggc
     4441 aggcaccagg aatcccaaaa acaggatcag cttgacggcg atgatattgg ccgtaatcaa
     4501 gcacgtcaca aagatcgcag cgatgacgac aaaccaggac gaatatttca cgtaaatggt
     4561 accctttctt tataatgtta taccatccgc ggcatacccg atggggtccg ctatgccgac
     4621 ctcggcaaaa gcgttgaggc gcaacaagca gctatcgcag gtgccgcagg ccagttcacc
     4681 gccctcatag cagctccagg tgaggtgata agggacggcc agttcgacgc ctttgcgaat
     4741 aatgtcggct ttgcccaaac gcatcagggg cgcgtgcagg tggaagcggc ggccatcctg
     4801 cgtgcttgcc ttggtggcga ggttggccat gtcttcgtag gcttgcaggt attcagggcg
     4861 gcaatcggga tagccgctat agtcgatggc attgatgccg agaaagatgt cgttcgcgcc
     4921 ggtcacttcg gcgtaggcca gggcaaatga gaggaagacg gtgttcctgg ccgggacgta
     4981 ggtgatgggg atatcggtcg ccatctccga caggctgcgc ctgtgcggca cggggatgtc
     5041 cgctgtgagc gcgctgcccc cgaaggcgcg caggtcgatg gcgatagtct gctgccgcgt
     5101 cacaccgtag tagcgggcga cggctgctgc ggcctccact tcgcgctggt gacgctgccc
     5161 atactcgaac gaaagggcga gcagttcaaa tccctgctgc cgcgccatag cgagggtggt
     5221 ggtcgagtcc agcccacccg ataaaagaac gacggccttg tgctgcatga tggctccttg
     5281 ctccttgcgt gtgctgcacc gggtaaaaat gcgaacgaac gagtatacgt ccaaataggg
     5341 catgaagaag ccccaacctg ttctgggct
//
LOCUS       DASZAK010000981         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_309006, whole genome shotgun sequence.
ACCESSION   DASZAK010000981 DASZAK010000000
VERSION     DASZAK010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309006"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..>2078
                     /locus_tag="VGT44_17170"
     CDS             100..>2078
                     /locus_tag="VGT44_17170"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HEV2582592.1"
                     /translation="MMPYLEKALNVRREELRPASLLFLYLFLAIGVYIMGQTVGDALF
                     LSAFPTYLPQVIIATAAVVGVFTSIYIRLSNRLRLERLIAGSLVFFALGFAFFWSLTR
                     FSGKWAYPFIYIWVYTIGALVPTMGWTLANYCLTTREARRIFGFIGAGAILGAPCAGF
                     LTADLTHRGVVKPETLLLVMATGLVACALSVNLLFRQRSQRQTGLNQAPGTMPGMPKN
                     LRQVWAHIRGSRYLLLITALILIGCASTTVIGYQFKLIAYKSFSGNKVALAAFFGRFN
                     GYIGLASFLLQILVTGRVLRSFGIRVTLFVMPTVFLGGSVGLLLAPVLLSASVLKGSQ
                     GLLRYSLDKSTTELLYLPVAPPAMKSQIKAFIDGFVWRMADGLAGITLFVFGNRMKFG
                     PGRISLVNFVFLSGWIVIAYAVRREYIAVLRGAIEQRTLDPERTAAGVLDATSAEVLA
                     HSLERRGEQQVLYGLSLFEIGRQPLWHPVLCSLLEHPSAVVRQRALHLLVDAGHQRIT
                     PQVEKMLGDEAPEVRAEALRYLVVHTHKDPLDLLQTSMEIPAHCLQSAVLMHLARSSE
                     PDYLPAARHILHTMVGGDGSEGLASRREAARALGSIPAPSELHAELFKLLCDQNPEVV
                     EQALLSAGRIRAGEFLPLVIEKLGQPRLLAAALTAL"
BASE COUNT          376 a          586 c          621 g          495 t
ORIGIN      
        1 ggcgtattac gcccatgaaa attcgcaggg actgccggcg cccgtgaagg tcgcaaattc
       61 cactcttgtc ccgaatcctg cgcgtagcgg gccgcagcca tgatgcctta cctcgagaaa
      121 gcgctcaatg tacgccggga agagctccgg cctgcttcgc tcctgttcct ttacctgttc
      181 ctcgccatcg gtgtctacat catgggacag acggtaggcg atgccctctt cctgagcgcc
      241 tttcctacct atctgccgca agtaatcatc gccactgccg ctgtggttgg cgtattcact
      301 tcaatttaca tacggctgtc taaccgcctg cggttggaac ggttgatcgc cgggtcactc
      361 gtgtttttcg cgctgggttt cgcgttcttc tggtcgttaa caaggtttag cggaaagtgg
      421 gcctatcctt ttatctatat ttgggtttac accatcggcg cgctcgtccc aactatgggt
      481 tggacgctgg ccaattactg cctgacaact cgcgaagcca ggaggatatt tggctttatc
      541 ggcgcagggg caattttggg cgcgccatgt gccggttttt tgacagcaga tctgacgcac
      601 cgcggcgtgg tgaagccgga aacgcttctc ctggtgatgg ccaccggcct ggtagcctgc
      661 gcgctatcgg tcaatctgtt gttccgccaa aggtcccagc ggcagacggg tttgaaccaa
      721 gctccgggga ccatgccggg tatgccaaag aatctgcgtc aagtgtgggc ccacatccgc
      781 ggttcacgct atttgctgct gataaccgcg ctgatcttga tcggttgtgc cagcacgacc
      841 gttatcggtt accaattcaa gctcattgcc tataagagtt tcagcggaaa caaagtggct
      901 ctggctgcat tctttggccg cttcaatggt tatatcggac tggcctcatt cctcctgcag
      961 atactggtta cgggtagagt gctgcgctcc tttggtatcc gggtgacgct tttcgtgatg
     1021 cccacggtat ttctaggcgg cagcgtggga ttattgctgg cgcccgtgct actgagcgcc
     1081 agtgtgctga agggcagcca gggactgctg cgctattccc tcgacaaatc cacgacggaa
     1141 ctgctgtacc tgcccgtggc gcctcccgcc atgaaaagtc agatcaaggc gtttattgac
     1201 ggctttgtgt ggcgcatggc ggatgggctg gccggcatta ctcttttcgt gtttggaaac
     1261 cgaatgaagt tcggtcccgg gcgcatcagc ttggtgaact tcgtttttct atctggctgg
     1321 attgtgatcg cttatgcggt cagacgggag tacatagcgg tgttgcgggg cgcgattgag
     1381 cagcgcacgt tagatccgga gcggaccgct gccggagtgc tggatgccac cagtgccgag
     1441 gtgctggcgc attcgctgga acggcgcggt gaacagcagg tgctctacgg gctcagcctg
     1501 ttcgaaatcg ggcgccaacc tttatggcac cccgtcctct gtagcttgct ggagcatcca
     1561 tcagccgtcg tccggcaacg agcgttgcat cttctcgttg atgccggaca ccagcggatt
     1621 accccgcagg tggagaagat gttgggagat gaagccccag aggtccgcgc ggaggcgctg
     1681 cggtatctgg tcgtgcatac gcacaaagac ccgctcgatt tgctgcaaac ttcgatggaa
     1741 attccggcgc attgcctgca atccgcggtc ctcatgcact tggcgcgctc gagcgagccc
     1801 gattacctgc ctgcggcccg gcacatcctg cacaccatgg tcggcgggga tggctcggag
     1861 gggttggcct ctcgacgcga agcggccaga gccctgggca gcatcccggc gccgtctgaa
     1921 ttgcacgcgg aattattcaa gttactgtgc gaccaaaatc cggaagtggt cgagcaagcg
     1981 cttctaagcg ccggtagaat tcgtgctgga gaatttctgc ctttggtcat tgaaaaacta
     2041 ggacaacccc gcttgctggc ggcggcgctg accgcgct
//
LOCUS       DASZAK010000982         5345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_524016, whole genome shotgun sequence.
ACCESSION   DASZAK010000982 DASZAK010000000
VERSION     DASZAK010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5345
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524016"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..1103)
                     /locus_tag="VGT44_17175"
     CDS             complement(75..1103)
                     /locus_tag="VGT44_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582593.1"
                     /translation="MTIKVGVIVGREWSFPPAFIDEVNKRDEGVEAEFVKLGGTRMDE
                     PIPYAVIVDRISHEVPYYRIYLKYAVLQGVTVINNPFMWTSDDKFFEAALATKLGVAS
                     PKTVVLPNKEYVPGIIHEESLRNLVYPLDWQAIVDYVGMPCVLKDAHGGGWKDVYICH
                     SMDELLFNYNRFGLRTAIVQEFIEWSEYVRCICLGQDEIMPIKYDPRERKYHVEHEFL
                     TPELGRRIVEDSRTLMRALGYDMNSFEWAIRDGVPYAIDFMNPAPDMDINSLTSHYFE
                     WVVKHMADLVIRMAKNPRSQSRPLRWDTMLSASREMPDDDSPQEQGNTPKTARKAVTT
                     TKRKVKES"
     gene            complement(1176..1895)
                     /locus_tag="VGT44_17180"
     CDS             complement(1176..1895)
                     /locus_tag="VGT44_17180"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEV2582594.1"
                     /translation="MNREYHRWYSRSLDRDMELLVFGHAGARVLVFPTSMGRFFQWED
                     MGMMATLGERLENGQIQLYCVDSVDGESWYAKQKYPAQRAQRHLQYDSYILNEVVPFS
                     KQRNGNPFLMSVGTSFGAYHAVNFAFRYPQLVDRCIGLSGFYDIRRFTEGYSNDDVYY
                     NNPVAFIGNEHDSARLDALRHMDIILAIGRDDPSCASTEEQSSVLWSKHIWHALRIWD
                     GWAHDWPWWKQMIDLYINGHD"
     gene            2311..5223
                     /locus_tag="VGT44_17185"
     CDS             2311..5223
                     /locus_tag="VGT44_17185"
                     /inference="COORDINATES: protein motif:HMM:NF040218.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582595.1"
                     /translation="MQRCAFCGFEMPANSHFCGNCGQAQSDETKSITQITTLSELHEA
                     AQNTPPLFSNPELPTLMATQAGYQDITRREPVSDHIALPPDFIFSEGRQTDERQFVLP
                     EMILPLASLASEAPAGQAPMVQGTPQISEVPHVQGTPSSSAGPPQGHVPLQHVSPPSA
                     PHAPAQSWQPSAPVHHPLEPAHHDPAQHPQHPQHSQHPQHPQHSQHSQQLHHHEEAQP
                     GHHEPRQEHEQEHAHHHAHHKAHRLHIRSTHSTAAHTATASKVVVGTATKWLIVAMTA
                     MVVVATSGVVFVLASAPGLSLSGSSSAVAVGNTLHIHGKGFLPGGSVVLTLDNGQPVA
                     LTPARLANTAQRGAAQAADDLLGMGYTASSSNASIPVNVAGSFDADVITLSSWSPGQH
                     IIHARENLGSRSADLQFTLLATPAKLIVKTASLTFKVPAGASESQSLDIGNAGQSNLN
                     WTAATDGSQWLILQGSAGVIGMNGQDANLNVTATTGHLSVGTYTASIQVNSNGGQAQV
                     PVTLQVLAPSAKKQAQLGVNPTSLNFGQLPSGQGQPPAQSLTISNLGTASLIWHASAG
                     SANWLTLSASGGTLNSGALPQTIQVKINTTGLAPGSYAAFLTITSNGGSAQVAVAVAV
                     TGTTPTPSPSPSPTPSPTPSPTPSPTPSPTPSPTPSPTPSPTPTPSPTPPPPTTWSVN
                     PNSLNQSSSGCSGGPTYTCTVTLTEGSSSPGNITWSASDSVGGVSFSPSSTTLTPGGS
                     QSVTISSIPCQNDTFTFTDNANVASPLTVSWTCQIQPPGGTASLGSCTYTSGTGWSCP
                     LVITANGNNQTSWPWSASSSGVNGITITPSSGSLTPGQSITATVAVPDMVCPASATIT
                     VSGGASNIPLNWSCAAPTITVTVNTPNCPSNNSGGWTCTDTVSLASGSQGQLTWTTSS
                     DLSGVTFSPAGGTLSVGQSVAVTITVPSSDCTNGTFYFAGTGGNTATVTWTCSSGG"
BASE COUNT         1174 a         1649 c         1414 g         1108 t
ORIGIN      
        1 cgttccgggt cacgcgccgc cacctgggcg caatgaattg cgcccctaca cgccacatca
       61 cccttgacta tgtactagga ctccttcact ttgcgttttg tcgtcgtaac ggctttgcgc
      121 gccgttttag gcgtatttcc ctgctcctgc ggcgagtcgt catctggcat ctcgcgactg
      181 gcgctcagca tggtatccca gcgcaaggga cggctctgcg agcgcgggtt tttcgccatg
      241 cgaatgacca ggtcggccat gtgcttgacg acccactcga aatagtggga ggtaagcgaa
      301 ttaatatcca tatcgggcgc gggattcatg aagtcgatgg cgtagggaac gccgtcgcgg
      361 atggcccact cgaaggagtt catatcatag cccagggcgc gcatcagtgt gcgcgagtct
      421 tcgacgatgc gcctgcccag ttcgggcgtg agaaattcgt gttcgacgtg gtacttgcgt
      481 tcgcgcgggt cgtacttgat cggcatgatc tcgtcctggc ccaggcagat gcagcgcaca
      541 tactcgctcc actcgatgaa ttcctgcacg atggcggtgc gcaggccaaa gcgattatag
      601 ttgaacaaca gctcatccat cgaatggcaa atgtacacgt ctttccagcc gccgccgtgc
      661 gcatccttca gcacgcaggg cattccgacg taatcgacga tggcctgcca gtccaacgga
      721 tagacgagat tgcgcaggct ttcctcgtgg atgatgcccg gcacatattc tttgttgggc
      781 aaaaccaccg ttttcgggct ggcgacgccg agtttagtcg ccagggccgc ctcgaaaaac
      841 ttgtcgtcgg atgtccacat gaaggggttg ttgatgaccg tcacgccctg caacacggca
      901 tacttcaaat agatgcggta atagggaact tcgtgcgaaa tacgatcgac gatgacggcg
      961 taggggatcg gctcgtccat gcgcgtgccg cccagcttga caaactcggc ctccacacct
     1021 tcgtcgcgtt tattgacttc atcgataaaa gcgggtggga aagaccattc ccgcccgaca
     1081 atcacgccta cttttattgt cataaacttc tactcctcta tgtgtatata tagggacaga
     1141 ttattttttc atttgtatga tagtatccct acacatcaat catggccgtt gatgtacagg
     1201 tcgatcatct gcttccacca gggccagtcg tgcgcccatc catcccagat gcgcagcgcg
     1261 tgccagatat gcttactcca cagtacgctc gactgttcct cggtactggc acaggacgga
     1321 tcatcccgac ctatggcgag gatgatatcc atatgtcgca gcgcatccag tcgtgcggag
     1381 tcgtgttcgt taccgatgaa ggcgaccggg ttgttgtaat agacatcatc gttggagtaa
     1441 ccctcggtaa agcgccggat gtcgtaaaag ccactcagcc cgatgcagcg gtcaaccagt
     1501 tggggataac ggaaggcgaa attcacagca tggtacgcgc caaaactcgt gccgaccgac
     1561 atcagaaagg gattgccgtt gcgttgtttg ctgaatggaa cgacttcgtt caaaatatag
     1621 ctgtcgtact gcaaatgtcg ctgcgcgcgc tgggccggat acttctgttt ggcataccag
     1681 ctttcgccat cgacgctatc gacgcagtac aactgaatct ggccattttc caggcgctcg
     1741 cccagcgtgg ccatcatacc catatcttcc cactggaaga atcggcccat cgaggtcggg
     1801 aagaccagca cgcgcgcgcc cgcgtggcca aagacgagca actccatgtc tcgatcaagg
     1861 gaacgactat accaacgatg atactcacga ttcatgcgac tctacactca catctggctc
     1921 tcagttgcgt aagccagctt cactgcgcag ccattccaac gccatgcctt tacagggaga
     1981 catagcgcag accgcgccct aaccaggtca cgcgaccctg ggtgaccggg gaaattgttg
     2041 acaaatacag gatgagtcaa tggctgagcg ccatcgcact cagcaaaatg gtatcactca
     2101 ttcccaggga ttgtcaaatg gcgcgcaatg gatgttcaga agggcaaata tactcgaaat
     2161 ctctcctgta aattctcaaa aaatatcgta tactattgaa atgggataga caactgcatt
     2221 catcaatctt gcaagttttc gtagagcgag ttgtaccgac tcggcagcta gcacacgtca
     2281 ggaatgagct tcctgagcct gggggatgct atgcaacgct gcgcattctg tggcttcgaa
     2341 atgcctgcca actcccactt ttgcggcaat tgcgggcagg cgcaaagcga tgaaacgaaa
     2401 agtatcacgc agatcacgac gctctccgag ctgcacgagg ctgcgcaaaa cactcctcct
     2461 ctgttcagca atccagagct tcccactctg atggctacac aagccggata ccaggacatc
     2521 accaggcggg agcctgtctc cgaccacata gctctgccac ctgatttcat cttctcagaa
     2581 ggtcgccaga cggatgaacg ccagtttgta ctaccggaaa tgattttgcc gctcgctagc
     2641 ctggctagcg aggccccggc aggccaggcg ccaatggttc agggaacacc ccagatcagc
     2701 gaagttccac atgtccaggg aacgccgtcg tcgtcggccg gccctccgca agggcatgtc
     2761 ccgcttcaac atgtcagccc gccttccgcg cctcatgccc cagcccagtc atggcagccg
     2821 agcgcgcctg tacatcaccc gttagagcca gcacaccatg accctgcgca gcacccacag
     2881 cacccacagc actctcagca cccacagcac ccgcagcact ctcagcactc tcagcaactg
     2941 caccaccacg aggaggctca acctgggcat cacgagcctc gccaggaaca cgaacaggaa
     3001 catgctcatc accacgctca tcataaagcg caccggctgc atatacgttc cacccactcg
     3061 acagcagcgc atacggctac tgcttctaaa gtggtggtcg gcactgcgac caagtggctg
     3121 atcgtcgcca tgactgcgat ggttgtggtt gctaccagtg gcgtcgtctt tgtgctagcc
     3181 agcgcgccgg gactctcgct gagcggcagc agcagcgctg tcgccgttgg caacaccctg
     3241 cacatacatg gcaagggatt tttgccggga ggcagtgtgg tcctgacgct cgataacggc
     3301 cagcctgttg cattgacgcc tgctcgtctc gcgaacacgg ctcaacgcgg cgctgcccag
     3361 gctgccgatg acctgttggg catggggtat acagcttcta gctcgaatgc ctctattcct
     3421 gtgaacgtcg ccggttcgtt cgacgcggat gtcataactt tgtcatcctg gtcgccgggg
     3481 cagcacatca tacatgccag ggagaacctc ggttcgcgca gtgcggattt gcagtttacg
     3541 ctgcttgcca ctcccgcgaa actcatcgtc aaaactgcct cgctcacctt caaagtgccg
     3601 gcaggcgcga gtgagagcca atcactcgat attgggaacg cgggccagtc aaacctgaac
     3661 tggactgccg cgaccgatgg gtcgcaatgg ctgatcctgc aaggtagcgc cggggtgatc
     3721 ggcatgaacg gccaggacgc caacctgaat gtgactgcca ctactggcca cctctcagtt
     3781 ggaacctata ccgcctccat tcaggtcaac tccaatggag gccaggctca ggtgccggtt
     3841 acgctgcaag tgcttgcgcc gagcgcaaaa aaacaggcac aactgggtgt gaatccaaca
     3901 tcgctcaatt tcggtcagct tccgtctggt caaggtcaac cgcctgcgca gtcgctcaca
     3961 atcagcaatc tcggcaccgc cagcctgatc tggcacgcca gcgctggcag tgcgaactgg
     4021 ctgacgctta gcgccagcgg cggcacactc aattcaggcg ctcttccgca aacgatccag
     4081 gtcaaaatta acacgaccgg cctggcgcct ggaagctatg ccgctttcct cacaatcacc
     4141 tcgaatggcg ggagtgcgca ggtagctgtg gccgtcgcgg taacaggtac aacgcctact
     4201 ccttcgccat cgccgtctcc aacgccgtct ccaacgccct caccgactcc gtctccaacg
     4261 ccgtctccga caccctcacc gacgccgtct ccaacaccgt ctccgacgcc gacgccgtct
     4321 ccaactcctc ctccgcctac cacgtggtcg gtaaacccga atagtctgaa tcaaagcagt
     4381 agtgggtgta gcggcggacc aacctacacc tgcactgtta ctctgacgga aggctcgagt
     4441 tcaccaggaa atatcacctg gtcagcaagt gatagcgttg gaggcgtgag cttctctccg
     4501 tccagcacaa cgctcacacc gggcggttct cagtcagtca ccatctcgtc tatcccatgc
     4561 cagaacgata cgttcacttt taccgacaac gcgaatgtcg ccagtccgct cactgtgtcg
     4621 tggacgtgcc agattcaacc gccgggcggt acagccagcc tcggttcctg tacctacacc
     4681 tccggtactg gctggagttg ccccctggtg attaccgcga atgggaacaa tcagaccagt
     4741 tggccctgga gcgcgtcaag cagcggagtc aacggcatta ccatcactcc atccagcggc
     4801 tcactgactc caggccaatc gattactgct acggttgctg tcccggatat ggtctgcccg
     4861 gccagcgcga cgatcactgt atcgggtgga gcgagcaata tcccgttgaa ctggagctgc
     4921 gccgcgccga cgataacggt cacggtcaat acacccaact gccctagcaa taattcgggt
     4981 ggctggacct gcacagatac ggtgtcactg gcctccggtt cgcagggcca gctgacgtgg
     5041 accacatcga gtgatctctc cggcgtgacc ttcagccctg ctggcggcac gttatcagta
     5101 ggtcaatcag tagctgtgac gatcaccgtt ccgtccagcg attgtacaaa cgggaccttc
     5161 tacttcgccg gcactggagg caatacggcc acggtgacgt ggacatgtag ctcaggcggg
     5221 tagtgcagcg taagctccag ggcgaccgca agggtctcca ccccactcca cgccgccccc
     5281 gcccttacaa tgatgaagaa ttaccttgta gcagaacttc gtagtcattg taagggcggg
     5341 ggcgg
//
LOCUS       DASZAK010000983         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_453358, whole genome shotgun sequence.
ACCESSION   DASZAK010000983 DASZAK010000000
VERSION     DASZAK010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453358"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..1877)
                     /locus_tag="VGT44_17190"
     CDS             complement(63..1877)
                     /locus_tag="VGT44_17190"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2582596.1"
                     /translation="MFDPDSELEARYSSVVATPDWYQTNIPCQVGCPAHTDVSTYIGL
                     ISQARFDEAYLLNRKCNIVPGVLGRTCARPCEPVCRRNKIDGKPIAICWLKRAAADHR
                     EFQHRAEKPPITKEKKVAVVGAGSAGLACARDLRDMGYPVTIYEADAVSGGVMVNGIP
                     VWRLPRHVTYEETTQYMEDMGVEVIYNTRVGRDIQVAELLDQYDAVFLAAGCYISNEL
                     TGPDNKIIPGSEYKGVQHGIKLLAKTNYGEPAFIGKKVIVLGGGFTAMDCCRTSVRFG
                     AEKVYVLYRRSKEEMGSDEYEVDEAEFEHVEFIYLVSQLEVLSKDGVHVSGLRVIRNK
                     LGDPDASGRRRPISIPGSEFTIEADTIIAAFGQYSDASWVQAKELNLEVNRRNGVPLI
                     DRETFMTNHARLFAGGDFTDGSRNLISAIGDGRDAAIAINRYLGGEDKPAEAAEEIEL
                     PDYRRGMVDDYESIPFQMMPGLPLKERYSNTRETETGYTPEEAVIQARRCLQCQLNIM
                     IDPSICILCSGCVDICPYDCISMEGLSRVVKGDPMHQGTERWRGGADMIIDEEKCIRC
                     GLCIVRCPTDAIAMVQFEVASPNDRWTVSKIPVLNINR"
BASE COUNT          436 a          657 c          609 g          442 t
ORIGIN      
        1 cacgatgcgc tggccaccag gacgaggaca ggcacaaggc ccccgctgct cctctccccg
       61 catcaccgat tgatgttcaa cactggaatc ttcgagacgg tccagcggtc gttggggctg
      121 gctacctcaa actgcaccat cgcgatggca tctgttgggc aacgcacgat gcagaggccg
      181 cagcgaatgc acttctcctc atcgataatc atatccgcgc cgccgcgcca tctctcggtt
      241 ccctggtgca tcggatcgcc cttgaccacg cgcgagagtc cctccatgct gatgcagtcg
      301 taagggcaga tatcgacgca gccggaacac aggatacaga tcgacgggtc gatcatgatg
      361 ttcaactggc attgcaggca gcgccgcgcc tggattaccg cctcttcggg cgtgtagccg
      421 gtctctgtct cgcgcgtgtt gctgtagcgc tctttgagcg gcaggccggg catcatctgg
      481 aacgggatgg actcgtaatc gtccaccata ccgcgccggt agtcgggcag ttcgatttct
      541 tcggcggctt cggcgggctt atcctcgccg cccaggtaac ggttaatggc aatcgcggca
      601 tcgcgcccat ccccaatggc ggagatgagg ttgcgcgagc catcggtgaa gtcgccaccc
      661 gcgaaaaggc gagcatggtt ggtcataaac gtctcgcggt caatcagggg tacgccattg
      721 cgccggttga cctccaggtt cagctccttc gcctgcaccc aggaggcatc gctgtactgg
      781 ccgaaggcgg cgatgatcgt gtcggcctcg attgtaaatt cgctgcccgg aatagaaatg
      841 gggcggcggc ggccactggc atcgggatcg cccagcttat tgcgaatgac acgcaggccg
      901 ctgacatgca ctccgtcctt gctcaagact tcgagctgag agacgaggta gatgaactcg
      961 acatgctcga actcggcctc atcgacctcg tattcgtcgg aacccatctc ctctttcgag
     1021 cggcgataca gcacatacac cttttccgcg ccaaaacgca ccgaggtacg gcagcagtcc
     1081 atcgcggtga aaccgcctcc cagcacaatc actttcttcc cgatgaaggc cggctcgcca
     1141 tagttggtct tcgccagcag cttgatgcca tgctgcacgc ctttgtactc cgagccgggg
     1201 atgatcttgt tgtccggtcc ggtcagctcg ttcgagatat agcagccagc cgccaggaag
     1261 acggcatcgt actggtcgag cagctctgct acctggatgt cacgtcccac gcgcgtattg
     1321 tagatgacct cgacgcccat gtcttccata tactgcgtcg tctcttcgta ggtgacatgg
     1381 cgcggcagac gccacacggg gatgccgttg accatcacac cgccggagac tgcgtcggcc
     1441 tcgtagatcg tcacaggata gcccatatcg cgcagatcac gcgcgcaggc caatcccgcc
     1501 gagccggcgc cgacaacagc caccttcttc tctttcgtaa tgggcggctt ctcggcgcga
     1561 tgctggaact cgcgatgatc ggcggcggcg cgtttcagcc agcagatagc gataggcttg
     1621 ccatcgatct tattgcggcg gcaaaccggc tcgcatggcc gcgcgcaggt gcgccccagc
     1681 acgccgggaa caatattgca tttacgattt aataaatagg cttcgtcgaa acgagcctgg
     1741 gaaatgagac caatataagt agatacgtcg gtatgcgccg gacaaccaac ctggcaagga
     1801 atgttggtct gataccagtc cggagtagcc acgaccgagc tatatcgcgc ttccaattcg
     1861 ctatcaggat caaacatggg gcctatcctt attcctggtg atatacgatt ccttcccaga
     1921 ttatagcaca gaggccaagt agcaacaaca cagatatggc gagcggtcag gtgtgaagcg
     1981 taaagggcga ccgcaagggt acgcccttac aaagacatcg aggtcggttc acgcatcact
     2041 ccgtcgtatc tttgtaaggg cgtacccttg cggtcgccct ctgagcctca tctgtccagt
     2101 tcagccaggc ggcagcctgg ttggggtctc atttttacgg cccg
//
LOCUS       DASZAK010000984         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_524057, whole genome shotgun sequence.
ACCESSION   DASZAK010000984 DASZAK010000000
VERSION     DASZAK010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524057"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1290
                     /locus_tag="VGT44_17195"
     CDS             <1..1290
                     /locus_tag="VGT44_17195"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV2582597.1"
                     /translation="SPISIFFNTIPQAGVVGAAIPLCYVIGFIIMLFVANQYSEFSRE
                     LPSSGSSYTFVNEGLGKNMGYLTAWFGIVAIVAGIPYTFTLLGVNMEALVLRLFGFDL
                     NWSFWFVLGIGIAFALCCWGARQSTNVDLTILVFEIGIALILAAIVLFQVGQKGGLPL
                     APFTFGAIPPGGNLGIGLILAILTFIGFEASAALGEETSNPRSNIPRAVFGSMLLVGV
                     FYVLMAYTGTVGYGLDKMVTGFANDAAPYDTISRHFSGPVLAVLVDIAGVLAYFSAAI
                     AIINSGARIIYKVGHDGLFPRWTAWLHPTRFTPVGAIAAICVLGLAVGLIAGFAMQPL
                     VAFGFWGTLSALFVMLIYVLVCVASIAFFWRKRRAQFNILRHGISPILGTITLGIVFV
                     LLLTAPASPPLNAIPFILGIWILLGIIMLFILRRKLA"
     gene            1337..>2295
                     /locus_tag="VGT44_17200"
     CDS             1337..>2295
                     /locus_tag="VGT44_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HEV2582598.1"
                     /translation="MSNDTDVVIVGAGLAGLVTTAELIDAGRRVIVLDQEPETSFGGQ
                     AFWSFGGLFLVNSPMQRRLGISDSHDLALQDWLGSAGFDREEDLWPRRWAEAYVDFAS
                     GEKYSWLHAQGVRFFPVVQWAERGGYLATGHGNSVPRFHVTWGTGPGVLEPFVRRVRE
                     GVQRGLVTLRFRHRATGLTMSNGVVDGVHGEVLEPGSFARGVASSRVVVGEFALRAQA
                     VIVTSGGIGGNHDLVRRNWPERLGAPPQRLISGVPAHVDGLMQEVVAASGGRIINPDR
                     MWHYPEGIHNFAPIWPNHAIRILSGPSPLWLDAHGQRLPIPLFP"
BASE COUNT          392 a          672 c          659 g          572 t
ORIGIN      
        1 tcacccattt ccatcttctt caacaccatt ccccaggccg gtgtcgtcgg agcggctatc
       61 ccgttatgct acgtgattgg atttatcatc atgctgttcg tcgccaacca gtacagcgag
      121 ttctcacggg aacttccctc cagcggttca tcctatacct tcgtgaatga gggattggga
      181 aaaaacatgg gttacctgac ggcctggttc gggatagtcg cgatagtcgc aggcatcccc
      241 tataccttta ccttacttgg cgttaacatg gaagcactgg tactgcgctt gtttggtttt
      301 gatctgaact ggagtttctg gtttgtcctg ggtattggca ttgcctttgc cctctgctgt
      361 tggggagccc ggcaatctac gaacgtcgac ctgaccatcc tggtctttga aataggtatt
      421 gctctcatcc tggcggctat cgtcctgttc caggttggtc aaaagggagg tcttcccctg
      481 gcgccattca cgttcggcgc gattccaccg ggaggcaatc tcgggatcgg gctgattctg
      541 gccatactca ccttcatcgg ctttgaggca tctgctgcac tcggtgagga aacaagcaac
      601 ccacgcagca atatcccacg cgctgtcttc ggatcaatgc tcctggttgg cgtgttctac
      661 gtgttgatgg cctatacagg tacggttggc tatggcctcg acaagatggt cacaggtttt
      721 gctaacgacg ctgcgccgta tgatactatt agccgccatt ttagcggccc agtcctggct
      781 gtcctggtcg acatcgccgg cgtcctcgcc tatttcagtg ccgccatagc gattatcaac
      841 agcggcgcgc gtatcatcta caaggtagga catgatggct tattcccacg ttggactgcc
      901 tggttacatc caacccgctt tactccggta ggtgcgatcg ccgcaatctg cgtactcggg
      961 ctggcagtcg gccttattgc aggcttcgcc atgcagcctc ttgttgcttt tggcttctgg
     1021 gggacgctta gcgccctctt cgtaatgctg atttatgtgt tggtgtgcgt cgctagcatt
     1081 gccttcttct ggcgtaagcg ccgcgcgcag ttcaatatcc tacgccacgg catctctccc
     1141 atacttggga cgatcacgct agggattgtc ttcgtcctcc tgctcaccgc acctgcctcg
     1201 cccccgctca acgccatccc tttcatcctg ggtatctgga tcttattggg catcatcatg
     1261 ttgttcattc tgcggcgcaa gctcgcatga cacgcaggat cccaccatgg cttgattcca
     1321 gggaggagag caatgtatga gtaatgatac cgatgtcgtc attgttggcg ctggtctggc
     1381 cggcctggtg accaccgcgg aactcattga cgcgggccga cgcgtgatcg tcctcgatca
     1441 ggagccagaa acatcattcg gcgggcaggc gttctggtcc ttcgggggcc tgttcctggt
     1501 caattcacca atgcagcgac gattaggcat cagcgattcc catgacctgg cgctgcaaga
     1561 ctggctcggc agcgccggat tcgaccgcga agaagatctg tggccccgcc gctgggcaga
     1621 agcctatgtc gatttcgcct cgggcgagaa atattcctgg ctacacgcgc aaggcgttcg
     1681 cttcttccca gtagtgcaat gggcggagcg cggcggctat ctcgcgaccg ggcatggcaa
     1741 ttcagttccc cgctttcatg tcacatgggg gacgggtccc ggcgtcttgg agccattcgt
     1801 tcgtcgtgtg cgggagggcg tgcaacgggg gttggtaacg cttcgcttcc ggcaccgtgc
     1861 caccggactc accatgagca acggcgtggt tgatggcgtc catggtgaag tgctggaacc
     1921 aggctccttc gcgcgtggcg tggcgagttc ccgcgtggtc gtgggtgagt tcgcgttgcg
     1981 ggcacaggcg gtgatcgtca cctcgggagg cattggtggc aatcatgacc tggtgcgtcg
     2041 caactggccg gagcgcctgg gcgcgccacc acagcgcctg atttcgggcg ttcccgccca
     2101 tgtcgatggg cttatgcagg aggtggtcgc cgcaagcggc ggacgcatca tcaaccctga
     2161 tcggatgtgg cattatccgg agggcatcca caatttcgcg ccgatctggc ctaaccacgc
     2221 tatccggatt ttgagcggtc cctcaccgct gtggctagat gcccatggcc agcgccttcc
     2281 aattccgctt ttccc
//
LOCUS       DASZAK010000985         6928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_237841, whole genome shotgun sequence.
ACCESSION   DASZAK010000985 DASZAK010000000
VERSION     DASZAK010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237841"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VGT44_17205"
     CDS             <1..230
                     /locus_tag="VGT44_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2582599.1"
                     /translation="IAFTAAVLGGIGNLVGAMLGGVLIGIISALSSLIPGSILPHGGD
                     AWQNAIIFAILILILVFRPSGLLGQRTPEKV"
     gene            291..2288
                     /locus_tag="VGT44_17210"
     CDS             291..2288
                     /locus_tag="VGT44_17210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2582600.1"
                     /translation="MSLSRSSNSLLPTPDTRRLLIKWVVALLAPVIVFTLWGGESISN
                     AVISPATAGGGVPTDTGSLIEAGVFTALFYAVVLLLASYLVAADSGRRGMIELWIDVI
                     VFTLVPLVLVILFSLILGIALSAIVWLVFFYVRNVVRKARHYTPPPPLESLKAINAEQ
                     RTELMSRAMLGGFWFGLAFALISLIVDLIYFISGSLPTILLIWAIVRTILLPVAGYFL
                     GRLAGTMALRRTLPQNGNGNVGTGANGSGNGSRRRQQLEALSTTRAREEAKDLVPNDL
                     PLRSAGAQRFFLLLLVAFVLFYPVLDPFLFGSGTDGRLAGYGDAGYYVILALGLNIVV
                     GFAGLLDLGYVAFFAIGSYAWAMIGSTQFGFLTGIHVNAQVWPWFFWPMLIGAALIAA
                     FFGVLLGAPTLRLRGDYLAIVTLGFGEIVPVVFTELDKYTNGVNGIVGIYSPILPGVD
                     WTSATPYYYLILILIALTILVNIRLRDSRLGRAWIAIREDEIAAASSGVNLVRTKLFA
                     FAAGAFFSGVAGAYHAAKLGSISPDSFSFGDSVVYLAMVVIGGIGSIPGVIVGAIAVY
                     AINEFILAQLDGLASDPSNILYTVHNAIASVVPGFTFGNIRNLVFGIVLVTIMIFRPE
                     GLIPSARRRRELQHGNDEAVEVGSLDVTPGTAEFEAEVRVE"
     gene            2309..3136
                     /locus_tag="VGT44_17215"
     CDS             2309..3136
                     /locus_tag="VGT44_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2582601.1"
                     /translation="MSIEQVIEHSDGPLLELNKVTKYFGGLAAVQEVDLKVWPKQIVS
                     VIGPNGAGKTTVFNLITGIYRPSSGDIRLAGQSLGGLKPDEVVKRGITRTFQNIRLFN
                     NMTVMENVLVGEHTQLKATVLGSVFHTPGVRHEEREARQRAVELLSFFGPVLVERQEE
                     YVINLSYADRRRVEIARALAAKPRLLLLDEPTAGMNEAETLEAVKYVRRLRDELGLTI
                     LLIEHKLAVTMGMSDSITVLDYGRKIAEGLPEDVRRDEKVIAAYLGKPTRTEQTTAE"
     gene            3229..3936
                     /locus_tag="VGT44_17220"
     CDS             3229..3936
                     /locus_tag="VGT44_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2582602.1"
                     /translation="MLRLIDVDTFYGPSQVLHNVSLEVNKGEIVCLLGANAAGKTTTM
                     KTIFGLVRPRHGAIEFEGKPIQRKLPGDIVRSGMALVPEARRIFPRMSVLENLEMGAF
                     INTNRAQVKEDIGHVCEIFPRIKERLKQVAGTMSGGEQQMLAMGRAMMSRPRMMCMDE
                     PSMGLSPILVETVFSTILRIRGEGVTIFLVEQNASMALSLADRGYVLQTGKIVLSDTA
                     PNLLTNDLVRQAYLGGG"
     gene            complement(4043..4219)
                     /locus_tag="VGT44_17225"
     CDS             complement(4043..4219)
                     /locus_tag="VGT44_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582603.1"
                     /translation="MKGQERSFEEEPDEEEYSDMELLERLESLREDMEDLGITSLAQL
                     IERIGELHRRLDRK"
     gene            complement(4223..4678)
                     /locus_tag="VGT44_17230"
     CDS             complement(4223..4678)
                     /locus_tag="VGT44_17230"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2582604.1"
                     /translation="MAKERLSEEHLGAWRAFLRAHAVTIDHIDHDMVASERLPLSSYD
                     VLIELYEAPEYRLRMHDLAERVVLSRSGLTRLVDRLEAEGLLTRDRSGTDRRGAYAVI
                     SEKGIAAMRRSWPVYSRGITEYFAQWLSIEEAAIIQQALERITQAGKKK"
     gene            4838..5389
                     /locus_tag="VGT44_17235"
     CDS             4838..5389
                     /locus_tag="VGT44_17235"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEV2582605.1"
                     /translation="MDWQFDAGHTHIGFTGRHLMVSTVRGAFERFEGSVQFDENDVVH
                     SQAEIHIEAASVNTHNTQRDEHFRSADFFDTAHYPYITFKSKRIVMRDAHHGQLIGDL
                     TIRDITKEVVLDGEFSGVSQSPWNTYSAGFNLRGKVNRKDWGLNWTMVLAGGGLVAGD
                     EITLAIDLELTKPVEVAALAQVE"
     gene            complement(5403..6737)
                     /locus_tag="VGT44_17240"
     CDS             complement(5403..6737)
                     /locus_tag="VGT44_17240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582606.1"
                     /translation="MKTHTRLLLLLSLLAGIIAACSPGHLGGSEIAFVRDGRLWTIDP
                     DGANAFEVVAATSPVIGYAWSPDHHMFVFRQLDRNSPGKSSNQTNINPLLGVPGDLPA
                     SLNTIGLDGGSPIQIIPSIAGLIQSNAWWNTSGTRLLYREITTNQSNQQPIDAQWWVS
                     QNDQPGGIARKSLPYSFSIPSFASDSSLAIGNSSQGIFTTTLAGDGFHLLVPGALPGH
                     PLPAALERLLWQPAHTRPALLYAILSPSSPSQPSSPLPPAVDLVLRNADGQVSPIARC
                     ACTQFAWSPDGNSILYSTGAAYTIYTLTPRASFAFSAEAGSVPYWSPDSRFILLDGPH
                     TLTLLATASHQQQLLLSDGSAVNTVPSMPDATALLQPASNNLWATDSLHFLLLTRGRT
                     LWQNRHLASGNGLYVATIDSAGHIQGSPTLIDSGNDTQPGWSYEDPNTSFLF"
BASE COUNT         1490 a         1728 c         1999 g         1711 t
ORIGIN      
        1 taattgcatt tacggcggca gtgctgggag gcattggtaa ccttgtgggt gcgatgctag
       61 gcggtgtgct gatcggtatc atttcggcgc ttagttctct catacccggt tccatattgc
      121 cgcatggtgg cgatgcctgg cagaatgcca tcatttttgc tatcctgatc ctgatcctgg
      181 ttttccggcc ctctggcctg cttggtcagc ggacgcctga aaaggtgtaa aggacgcgag
      241 catgtttcaa tgttataaac atacaaagcc ggaaatgagg tgatgctgcc gtgtctctct
      301 caagaagctc caatagcctg cttccaacac ccgatacgcg caggttattg ataaaatggg
      361 ttgtggcctt acttgctccc gtgattgtgt ttactctctg gggtggcgag tcgatcagta
      421 atgctgtgat atcgcctgca accgctgggg ggggtgttcc aactgatact ggatcactca
      481 tcgaggccgg tgtttttact gctctcttct atgccgtcgt tctgttgctt gcaagctatc
      541 ttgtagcggc tgatagtggg cgtcgcggca tgattgagct gtggattgat gtaatcgttt
      601 ttacactggt tccgctcgta ctcgtgatcc tttttagcct gatattaggg attgctctgt
      661 ctgccattgt ctggttggtg ttcttctacg tccgcaacgt cgtgcgtaaa gcgcgccact
      721 atacccctcc gccaccgtta gagagcctga aggcgatcaa cgccgagcag cgtactgaat
      781 taatgagccg ggctatgctt ggaggtttct ggtttgggct tgcgttcgcg cttatttcct
      841 tgattgttga tctcatctat ttcatctcag gctcgttgcc gaccatcctc ttaatctggg
      901 cgattgttcg taccattctt ttacccgttg caggctattt tctagggcga ctggccggca
      961 cgatggccct gcgtcgcaca cttccacaaa atggaaatgg caatgtcgga actggtgcga
     1021 atggaagtgg gaatggctcg cgccgacgac aacaactgga agcactttct actactcgcg
     1081 ctcgtgagga ggcgaaggat ttggtaccca acgatctacc cctcagaagc gcaggggcgc
     1141 agcgcttttt cctgctgctg ctcgtggcct ttgtactctt ctatcccgta cttgatcctt
     1201 tcttatttgg ttccggcaca gatggtcgcc ttgccgggta cggggacgcg gggtactacg
     1261 tgatcctggc gctcggactg aacatcgtcg ttggtttcgc gggactgctc gacctgggtt
     1321 acgtggcttt ctttgccatt ggttcctatg cctgggctat gattggctcc acgcagttcg
     1381 gctttcttac gggtattcat gtgaacgccc aggtctggcc ctggtttttc tggccaatgt
     1441 tgattggagc tgcgctcatc gcggcctttt tcggagtgct acttggcgcg ccaacactgc
     1501 gcttacgagg cgactacctg gcgattgtga cgcttggctt cggtgagatt gtaccggttg
     1561 tctttacaga attagataag tacacgaacg gcgttaatgg tattgtcggt atctactcgc
     1621 ccatattgcc tggcgtcgat tggacctccg ctacccccta ctactacctg attctcatac
     1681 tgattgccct caccatcctt gtcaatatcc gcctgcgcga ctcgcggctg ggacgcgcct
     1741 ggatcgccat tcgtgaggat gagattgccg ctgcctcttc gggcgtcaat ctcgttcgaa
     1801 ccaaactatt cgcctttgcg gcaggcgcat tcttctcagg tgtcgcgggg gcctatcacg
     1861 ccgccaaact cggcagcata tcgccggata gcttcagttt cggcgattcc gtggtctacc
     1921 tcgccatggt agttatcggc ggcataggca gtatccccgg tgtgatcgtg ggagcaattg
     1981 cggtctatgc gatcaatgaa tttatcctgg cgcaactcga tggtctggct tccgacccga
     2041 gcaatatact ctatacagtt cacaatgcga ttgccagtgt cgttcctgga tttacctttg
     2101 gcaatattcg caacctggtc tttggcatcg ttcttgtgac gatcatgatc ttcaggccag
     2161 aaggacttat tccaagtgcg cggagacgac gcgaactgca acatggaaac gacgaagcag
     2221 tagaagtagg gtcgttggat gtgacgcctg gaacggctga attcgaagct gaggtgcgcg
     2281 ttgagtaaca accagtgggg agttttcaat gagtattgaa caagtcatcg agcattctga
     2341 tggtccactt ctagaattga acaaagtaac gaaatacttt ggggggctgg cagcggtgca
     2401 ggaggttgat ctcaaggttt ggccgaaaca gatcgttagc gtcattggtc ccaatggcgc
     2461 cggaaaaaca acggtcttca acttgattac cggtatttat cgaccgtctt cgggtgatat
     2521 tcgtctcgct ggtcaatcat tggggggatt gaagcctgac gaggtagtca agcgcggcat
     2581 aactcgtaca tttcagaata ttcgcctatt caacaatatg acagtcatgg agaatgtgct
     2641 ggtgggagag cacacgcagc tcaaggcaac cgtcttgggt tccgtctttc acacaccagg
     2701 ggtgagacat gaagagcgag aggctcgcca gcgggctgtt gagttactca gcttctttgg
     2761 ccccgtcctg gtggagcgac aggaagaata tgtgattaat ctctcttacg cagatcgtcg
     2821 gcgcgtcgaa attgcgcgcg cgctggcagc taaacccagg ttactcttgt tagatgagcc
     2881 aaccgcgggc atgaacgagg cggagacgct tgaagccgtg aagtacgtgc gccgtttgcg
     2941 cgatgaatta ggtctgacca tcctgttgat cgagcataag ctggcggtga cgatgggcat
     3001 gtcggatagc ataactgtac tcgattatgg gcgaaagata gcggagggct tgcccgagga
     3061 tgtgcggcga gatgagaagg tcattgccgc ctaccttggc aaacctacga ggacagagca
     3121 aacgaccgct gagtagctct caatttggag gaaaatcgtt aaatgaccag tgctgatatt
     3181 tcttcgtctc cttctgacca ggacaagacg gaagcccaaa aatcgacgat gttacgatta
     3241 attgatgtgg atacgttcta tggtcccagc caggttcttc ataatgtttc gctcgaagtg
     3301 aacaaaggtg agattgtctg cctgctggga gcaaatgcgg ctggcaagac aaccaccatg
     3361 aaaaccatct ttggactggt acgaccgcga cacggcgcaa tcgaattcga ggggaaaccc
     3421 attcagcgca aactccccgg cgatatcgtt cgttctggca tggcgctggt tcctgaagct
     3481 cgccgcattt tcccacgcat gtcggtgtta gagaacctgg agatgggagc tttcattaac
     3541 accaaccggg cccaggtgaa ggaagacatc ggacacgttt gcgagatttt cccgcgcatc
     3601 aaagagcggc tgaagcaggt tgcaggaacg atgtctggcg gcgagcagca aatgctggcg
     3661 atgggccgcg cgatgatgtc tcgaccgcgc atgatgtgca tggatgagcc gtcgatgggt
     3721 ctctcaccta ttcttgttga gacagtcttt agcactattc ttcgcattcg tggcgagggg
     3781 gttacaatct tcctggtgga gcaaaatgca tcgatggccc tgagccttgc tgatcggggc
     3841 tacgtcttac agaccgggaa gatcgtcctg agtgatactg caccgaatct gctcacaaac
     3901 gacctcgtac gccaggcgta cctcggcggc gggtagaggc cctcgcgttc aaatgtctga
     3961 acgaggtatc actagcatca taagtctggt tgattgatag aacccacatc ccgcagactt
     4021 tttggggcgc ttaaatggca ggttatttgc gatccaatcg tcgatgaagc tctccgatac
     4081 gctcgatcag ttgcgccagg gaggtgatgc ccaaatcctc catatcctcg cgcaaggatt
     4141 ccaggcgttc caacaactcc atgtcgctat attcttcttc gtcgggttcc tcttcgaagg
     4201 atcgttcttg ccctttcatc ggttatttct tctttccagc ttgagttatg cgctcaagag
     4261 cctgttgtat aattgctgct tcctctatgg agagccactg cgcgaaatat tctgtaatcc
     4321 cccgtgaata gaccggccat gagcgccgca tagccgcaat acctttttca ctgatcactg
     4381 cgtatgcgcc gcgtcgatcc gtgccgctgc gatctcgcgt caacaatcct tcggcttcga
     4441 gtcgatcaac caggcgtgtt aacccgctcc ggctcaagac gacgcgctcc gccaggtcgt
     4501 gcattcgcaa acgatactca ggagcctcgt acagctcaat gagtacgtcg taggatgaaa
     4561 gcggcaggcg ttcggatgcc accatatcat gatcgatatg gtcaatggtg acggcgtgcg
     4621 ctctgagaaa ggcgcgccag gccccaagat gttcttctga tagacgttcc tttgccatac
     4681 tccataatat aacataaaga tgttgtctcc gcaatcattg acaatacaat gatatatttg
     4741 ctatgataat aagtggttgt atatgcaact atacgcaaag caacgatctc tcttgagaga
     4801 tcgttgtcgc aactcaagca catcaaggag tttattcatg gattggcaat tcgatgctgg
     4861 ccacacacat attggcttta ccggtcggca tttgatggta tcgaccgtgc ggggagcatt
     4921 cgagcgcttt gagggttccg tacagttcga cgaaaacgat gtagtgcact cacaggcgga
     4981 gattcatatt gaggccgcaa gcgtcaacac gcataatacc cagcgcgacg agcatttccg
     5041 gtcggcggat ttcttcgata cggcgcatta tccgtacatc accttcaaaa gcaagcgcat
     5101 cgtcatgcgg gacgcgcatc acgggcaact catcggtgat ctgaccatcc gcgatatcac
     5161 aaaggaagtg gtcctggatg gcgagttcag cggggtgagc cagagcccct ggaacacgta
     5221 cagcgctggc ttcaatctgc gcggcaaggt taaccgcaaa gattgggggc tgaattggac
     5281 catggtgctg gcaggtggcg gcctggtcgc gggcgatgaa atcacactgg cgatcgacct
     5341 ggaactgacg aagcctgtag aagtggcggc gctggctcag gtcgaataag aagcgtgatt
     5401 ttttagaaca aaaacgacgt attcgggtct tcgtaactcc atccaggctg tgtgtcgttg
     5461 ccgctatcga tcagtgtggg gctgccctgt atgtgcccgg cgctatcgat ggtcgcgaca
     5521 tacagcccgt tgccgcttgc gagatgccgg ttctgccaga gtgtgcgccc acgagtgagc
     5581 aagagaaagt ggaggctatc ggtggcccac aggttgttgg atgctggttg cagcagagct
     5641 gtggcatccg gcatggatgg cacggtattc accgccgatc catcgctgag caagagttgt
     5701 tgctggtgac tggccgttgc aagcagtgtg agcgtgtgcg ggccatccag caggataaac
     5761 cggctgtccg gcgaccagta ggggacgctg ccagcctcgg ctgaaaaagc gaaggatgcc
     5821 cgcggggtga gggtatagat tgtgtaggct gcaccggtgg aatagaggat ggagttgcca
     5881 tccggcgacc aggcaaactg tgtgcaggcg cacctggcga taggtgagac ctggccgtcg
     5941 gcgttcctaa gaacgagatc gacggcggga ggcaacgggc tggatggttg cgagggagag
     6001 gagggggaaa ggatggcgta gagcagcgca ggccgggtat gtgccggttg ccagagcagg
     6061 cgctcgagcg cggcaggcag cggatggccg ggtagcgcac ccggcacaag caagtggaac
     6121 ccatcacctg caagcgttgt tgtaaaaatg ccctgcgaag aatttcctat agccaaagat
     6181 gaatcggacg cgaaagatgg gatggagaag gaataaggga gggatttgcg ggcgataccg
     6241 ccgggctgat cgttttgcga aacccaccat tgcgcatcga taggctgttg gtttgattgg
     6301 ttcgtggtta tttctctgta aagcaacctg gttccgcttg tattccacca ggcattgctc
     6361 tggattaacc cggcgatcga cggtatgatc tggatgggtg atccaccatc caggccgatg
     6421 gtattgagcg aagcgggaag atcgccaggg acccctaata gcggattgat gttggtttga
     6481 ttggaggact tgccaggact attcctatcg agctgtcgaa agacgaacat atgatggtct
     6541 ggcgaccatg catagccgat caccggtgaa gtggctgcga ccacctcaaa ggcgttggcc
     6601 ccatcggggt ctatcgtcca gagacgcccg tcgcgtacaa aggcgatttc gctgccgccc
     6661 aggtgtccgg ggctacaggc tgcaatgatg cccgccaaca gggacaggag taaaagcagc
     6721 cgtgtgtgtg ttttcatcgt ttgcatgacc agagtatagc tttgcggtga tggagcgtca
     6781 atagctgagc gtgaggcgct gcaagcctta ctattgacgt aaccttgctg gtctactatg
     6841 ctaatgatgt gacctgatta cagctgtagg ggtgagcctt gatggtcgcc agcctttact
     6901 taaccttcga cggaatcggc ttgcaatg
//
LOCUS       DASZAK010000986         9846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_381748, whole genome shotgun sequence.
ACCESSION   DASZAK010000986 DASZAK010000000
VERSION     DASZAK010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381748"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            439..681
                     /locus_tag="VGT44_17245"
     CDS             439..681
                     /locus_tag="VGT44_17245"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEV2582607.1"
                     /translation="MPPVDNVDRTLDCLKLLCPVPVIKTSKTVKEMQVGQVLLLLADD
                     PGSKADMVAWARQTGNELLGIEEEGKTFRFYIRKKK"
     gene            779..1159
                     /locus_tag="VGT44_17250"
     CDS             779..1159
                     /locus_tag="VGT44_17250"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEV2582608.1"
                     /translation="MDDENKRLLYVQTNGVESPERAATPFYLAASAAAMDIEVGIYFT
                     VNGPTLLVKGVAEELIPKKDGKGAPLRRFIDQAAELGVKFYVCQPSLDLSNLHYEDLI
                     DGVEMIGGAAFNDMALEADAVISF"
     gene            1212..1904
                     /locus_tag="VGT44_17255"
     CDS             1212..1904
                     /locus_tag="VGT44_17255"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEV2582609.1"
                     /translation="MAQVTAKRADVPYEEKERLFTQVTQDIRFHDYLKGCYECGICVA
                     ACPSARFYDFSPRKITQAVAREDIDLVYKQMNEDVWNCSQCFSCDRCPRGNSPGGLIT
                     IMREVAVKNGLHSAKEALEGYGRVVYKIMSTGTQVSPDMLQPDAFPDWGPEVKDVADN
                     LDIWRRALPPETLHTTELAWDVDEKTLTELYLIWHMTGVLDMIKEIDEGLHMILQDVM
                     EDALEEKGFSLS"
     gene            1965..3179
                     /locus_tag="VGT44_17260"
     CDS             1965..3179
                     /locus_tag="VGT44_17260"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEV2582610.1"
                     /translation="MTSDVPIPIANVTRGTIPQRVFQRDPANAPTEDIREQIFELEAQ
                     GEWIVQRVPEDSVEVMTKYGRTKHIPRQYTWHHKSCGQCGHIPGYSTAIFWLHRQLGM
                     DYNDPRDQTSCTAWNYYASATSNAAAQAAVAVRNFAAAYETGYFPMIHCGTSYGHYKE
                     TREQLVHHPELRKQVRDIMAKLGKPLVIPEEIVHYSEWVHTMRFEIEKRRVFDLSNIT
                     VTVHPACHYYKLVAEDAIYDPDLYGGQRTAVITAVVETLGAKVADYSTWFDCCGFGFR
                     HILVQRDFTRSFATLRKIERMKEEANPDVTLTHDTGCVTTLDKSQFAARAHGRNVGIP
                     VLSEAQFAALAMGAHPYRVCQLHWHSVDYRPLLEKMGIDWEARWAEFQGDLEQLRRGE
                     KQFLSWEDADAR"
     gene            3169..4263
                     /locus_tag="VGT44_17265"
     CDS             3169..4263
                     /locus_tag="VGT44_17265"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2582611.1"
                     /translation="MQGDTVLIIGGGPAGLYAARSVADLGTPVVLVEKRERLGGAPIS
                     EHYASFTPHLEDAETAMNRMASDISDDPLIEVLLNTRVTAGSGSPGDFAVTLTGRSGE
                     TRELRAGSIIVATGFDHFDPGRETQMYGYYEFEDVLTLVDAEKMLKAKRFVRPSTGEP
                     PKRVCFIQCVGSRDRRLGNEYCSKVCCGVASKEAIEIKELLPDTKVFIFYIDMRMYGF
                     WEDQLYWKAQEEHKVNYIRGIVTEVLRRGDSLVIKGEDTTMGRPVELPMDVVILSVGM
                     VPSAGTIEIAKVLNLPRERHGFLETVGAPLDTVTTPVPGIFVAGAAAGPKDIEDSISM
                     AGTAAMKAVSTVRRHLRQVATPLSAGGGGE"
     gene            4266..5039
                     /locus_tag="VGT44_17270"
     CDS             4266..5039
                     /locus_tag="VGT44_17270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582612.1"
                     /translation="MPAPASNHGETVGQVVDTPSALEFMREALEKITEDELAAIVAEL
                     RAKSQRVRQMFALNMLADFDESKFRSLLRSIFSTRRRAAQVMALVDAGAFRDAASELL
                     HGTAPLPERFDAFVARMEPLDLATRCDLAGECLHYWDFERYWLWTRWMWDPDRNTGSL
                     PLVTIQEYDLHGETPGLTYVKVGEAVAYVNGVGEAAGFQHGGRGLLGTDVYLACVYVV
                     YLYTVTRMRMTKEFNKVIPQFPELARRLLGTYKMEGMKS"
     gene            5036..6304
                     /locus_tag="VGT44_17275"
     CDS             5036..6304
                     /locus_tag="VGT44_17275"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV2582613.1"
                     /translation="MTAVEPERIHEKKVAIQDGVDISGHWNRMFDERVIWDYDTSKLD
                     EVTQIEGAESLGACYQCGKCTPVCPVDHVGDYSPRKIFRKTQMGISLLTSSDLWLCTT
                     CANCLRVCPKEVNMIKIMPAVREQAVLAGNAPTELSQAFENTFRYGNPLGEPTRKRAD
                     WARDAGVPVPILAQIRRPVDVLWFVECYPAYHPRGKDAARGLARIFHALGVDYGILGV
                     EEKCSCDSQRLAGEKGLFEMHTEHNIKMFGKYQFNTLVTADPHALNAFKKEYPKFGGE
                     YNVLHYTQFLAPMLDRIPFKKSLPYRVTFHDPCYLGRHNGEYEAPRRLLEAIPGIELT
                     EMARCKANGYCCGGGGGGMWLDSFARPHVSERLSERRVREAVSTGADVLAVCCPYEIS
                     RFEDAVKSTGNDGKLIVRDIIELIDESMRG"
     gene            6330..7109
                     /locus_tag="VGT44_17280"
     CDS             6330..7109
                     /locus_tag="VGT44_17280"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEV2582614.1"
                     /translation="MHIIVPVKQVPDLVEELEINSDGTDLERDMLKYKINEWDESALE
                     EALQLKELHGGTVTVLTVASGDVDVDETLYTCVAKGADRVAKIVGDFEGHYDSHTAAR
                     ALAEAIREQPYDLILTGVQAADDLDGQVGVLLATYLGIPHISVVSGIEVDPLSRTATV
                     HQEYAGGIMATFEADLPLVLGVQASRAIPRYAPVSRVRQAMKTARIEEIEATTPAGAG
                     ISVRRMFKPESTEHAEMLEGDTEEVVDRVIEILRERGLWRA"
     gene            7112..8089
                     /locus_tag="VGT44_17285"
     CDS             7112..8089
                     /locus_tag="VGT44_17285"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEV2582615.1"
                     /translation="MAEHDVFVIAEHLEGKLSDVTFEMLGKARSLAPALGGRAVAVLL
                     GHEATALAGQLGAADQVLYIDDAGLRYFVPERAEQALRTVLSNEKPALTMIANTSMGM
                     DLAAALSAALDWPLIAYCSDAQAQDGKIVATSQIYGGKIAVETRSESASTLVSVLAGA
                     FPASEGHADRPAAIQTIFGAANEGRVRFKGLTVPEAGDVDITREAFLVSVGRGIGSKD
                     NIELVEELANTLGASLAASRPIIDSGWLPKQRQVGKSGLTVKPKLYMAVGISGAPEHL
                     QGMRDAELIIAINSDAGAPIFEVADYGIVGDLFDIVPALTERLAEVRAK"
     gene            8158..>9846
                     /locus_tag="VGT44_17290"
     CDS             8158..>9846
                     /locus_tag="VGT44_17290"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEV2582616.1"
                     /translation="MSVILASSPIGPGRPIFEFFTPVMEVIFYSLAAISTLVFLLGVF
                     LKVRKYWRGKSEMRLDHLPRRLGRAIRLVAQNATVGRRDRYAGVGHFMIMWGFITLFL
                     GTVILALDYDLIRPIHPDWQFYHGDFYDIYKLVLDTLGSAFLIGLLAMMIRRGFFRLP
                     KLNYKRVDRPDGSYDRHLFKVGDALFVGGLFLIGLTGYLLEGARIAHDQPVTMAWSPV
                     GSLVSQFYLWIGLTPPQLVISRVTLWWIHVSLALAFVAYIPFSKAVHMLADFVNLTFR
                     DEKAGIALPPIAEEPAPAHPGYRDIRDFTWKHLLDLDACTKCGRCHEACPARASGAPL
                     SPRDLILDLRDCADEQLSFKAPLSQRTLPVLPVDHRQNGHNGSSPHHRPVAGGVIKAE
                     TLWACTTCRACVEACPVGIEHVPIIVEMRRSLLDEGEMDPKLQEALTNLSKYGNSFGV
                     SDRLRAKWTKTLPFKIKDARKEPVEYLWFVGDFASFDQRVQDITRKVATVFQQVALDF
                     GILYEGERNSGNDVRRVGEEGLFQMLAEQNIKSLGKATFKTIVTTDPHSLNALRN"
BASE COUNT         2087 a         2901 c         2913 g         1945 t
ORIGIN      
        1 tcacttcgac gattgtcacc actggccaga gtacaccggt ctttctcgtc acgggaggag
       61 agatcatcgt ctacacggcg tatagtgttc tcctgatgcg tcggatgaaa ccgcaggcgt
      121 ccggggcagc aaggggggca acgtctacgc caacactcgc acaggaggaa gtggcgtaag
      181 attaacagct catggtatgg gctcgatctt tccgaacgcc gcgtaagagg tcgagctcat
      241 ctgctttggt gtaacatatt tggcaaaagt agcacttgac ttttccgcat acagcgatta
      301 caatacttgc atactttcgt tatttctaaa gtatgaacgt aaccgggcgg cttatcctct
      361 ctctcgttcg cagcaatgtg agcgggagca tcgcatataa caacctttgt acacagtcgt
      421 acataccggg aggtaactat gccaccagtt gacaacgttg atcgtaccct cgattgtttg
      481 aaactcctct gtcctgtacc cgtcatcaag accagcaaaa cggtgaagga gatgcaggtg
      541 ggccaggtct tgctactgct cgccgacgac cccggctcga aggccgatat ggttgcctgg
      601 gccaggcaga ccggcaacga actgctgggc atcgaagaag aaggcaagac cttcagattt
      661 tacatccgca aaaaaaagta atgccattga agcgcatcag catggtagag gctggtaaag
      721 ccggtgcagc cagcaaaggc tatgccatgc tctattctcc cgggaaaggt gaagccgaat
      781 ggatgacgaa aacaagcggt tactgtatgt ccagaccaac ggcgtagaat cgccggagcg
      841 cgcggccacc ccgttctacc tggcagcctc tgccgccgcc atggatatcg aggttggcat
      901 ttatttcacc gtcaacggtc ccacgctgct tgtcaaaggt gtagccgaag aactgatccc
      961 caaaaaggac gggaaaggcg cgccattacg ccgttttatc gaccaggctg ctgaactcgg
     1021 cgtcaaattc tacgtctgcc agcccagtct ggacctgagc aatctacatt acgaagattt
     1081 aatcgacggc gtcgaaatga ttggcggcgc tgcctttaac gatatggcgc tcgaagccga
     1141 cgcagtgatc tcgttttaag agatcgctgt ccgtcgcaaa gtgtgtcata ggcccacatg
     1201 gaggtgttcc attggcccag gtaacggcaa aacgcgctga tgttccttat gaagagaagg
     1261 agcggctctt cacccaggtc acgcaggaca tacgctttca tgactacttg aaagggtgct
     1321 atgagtgcgg tatctgtgtg gcggcctgtc cttccgcccg cttctatgat ttcagcccgc
     1381 gtaaaatcac acaggcggtg gcccgcgaag acatcgatct cgtctacaag cagatgaacg
     1441 aagatgtgtg gaactgctcg cagtgctttt cctgcgatcg ctgcccgcgc ggcaattccc
     1501 ccggcggtct catcaccatc atgcgcgagg ttgcggtcaa aaatggcctg cactccgcga
     1561 aagaggcgct cgaaggctac gggcgtgtcg tctacaagat catgtccacc ggaacgcagg
     1621 tctcgcccga catgctgcag cctgacgctt tccccgactg ggggccagaa gtgaaggatg
     1681 tcgccgacaa ccttgatatc tggcggcgcg cgctgccccc agaaaccctg cacaccaccg
     1741 aactcgcctg ggatgtcgat gaaaagacgc tgaccgagtt gtatttgatc tggcacatga
     1801 cgggtgtcct cgacatgatt aaggaaatcg acgaagggct gcatatgatt ttgcaggacg
     1861 tgatggaaga tgcgctagaa gaaaaaggct ttagtctctc ttaaggctct tgaccgccag
     1921 aagcggctga tatgctcacc ggctgagacc ggttgggagg taatatgacc agcgacgttc
     1981 ctatacccat cgccaatgtc acgcggggca ccatcccaca gcgggtgttc cagcgcgacc
     2041 ccgccaatgc gcccaccgaa gatatccggg agcagatctt cgaactcgaa gcccagggcg
     2101 agtggattgt gcagcgcgtc cccgaagaca gtgtggaagt tatgacgaag tacggtcgca
     2161 ccaagcatat cccgcgccag tacacctggc atcacaaaag ctgcggccag tgcggtcata
     2221 ttcccggcta ttcgaccgcc atcttctggc tgcaccgcca gctcggcatg gactacaacg
     2281 acccgcgcga ccagacctcc tgcacggcct ggaactacta cgcctccgcc acctccaatg
     2341 cggcagccca ggcggccgta gccgtgcgca acttcgccgc cgcttacgag accggctact
     2401 tccccatgat tcactgcggc acaagctatg gccactacaa agagacgcgc gagcagctcg
     2461 tgcatcatcc cgaactgcgc aaacaggtgc gcgacatcat ggcgaagctg ggcaagccgc
     2521 tggtcatccc agaggaaatc gtccactaca gcgaatgggt gcatactatg cgctttgaga
     2581 ttgagaagcg cagggtattt gatctgagca acataacagt caccgttcac cccgcctgcc
     2641 actattacaa gctcgtggct gaagatgcca tctacgatcc cgacctctat ggaggacagc
     2701 gcaccgcagt gatcaccgcc gtcgttgaga cccttggagc gaaggtcgcc gattactcca
     2761 cctggttcga ctgctgcggt tttggcttcc gccacatcct ggtgcagcga gacttcacgc
     2821 gctcattcgc cacgctgcgc aaaatcgagc gcatgaaaga agaagccaac cctgatgtca
     2881 cgctcaccca cgataccggc tgcgtaacca ccctggataa gagccagttt gccgcgcgcg
     2941 cgcacgggcg caatgtcggc atcccggtcc tctccgaggc gcaatttgcg gcgctggcga
     3001 tgggagcgca cccctatcgc gtctgtcaat tgcactggca cagcgtggat taccgcccac
     3061 tgctggaaaa gatgggcatt gattgggagg cacgttgggc cgaattccag ggagaccttg
     3121 agcaattgcg caggggcgaa aaacaatttt tatcatggga ggatgccgat gcaaggtgat
     3181 accgttctga ttattggcgg cggccccgcc gggctgtatg ccgcgcgaag tgtcgctgat
     3241 ctgggtacgc ctgtggtgct ggtggagaaa cgcgagcgcc tcggcggggc gcccatatcg
     3301 gagcattatg cctcgttcac cccccacctg gaggatgccg aaacggccat gaatcgcatg
     3361 gcgagcgaca tcagcgatga cccgctgatt gaagtactcc tgaatacgcg ggtgacggca
     3421 ggttcgggca gcccgggcga ttttgcggtc acgctgaccg gcaggagcgg tgagacgcgc
     3481 gagttgcgcg ccggttcgat catcgtggca accggcttcg accatttcga ccctggccgt
     3541 gaaacgcaga tgtacggcta ctacgaattc gaggacgtgt tgacgctcgt ggatgccgag
     3601 aagatgctga aagcgaaacg cttcgtgcgc ccctccaccg gggagccgcc aaaacgtgtg
     3661 tgcttcatcc aatgcgtcgg gtcgcgcgat cgccgcctgg gcaacgagta ctgctcgaag
     3721 gtctgctgcg gcgtggcctc taaagaagcc atcgagatca aagaactgct ccccgacacg
     3781 aaagtcttca tcttctacat cgacatgcgt atgtacggct tctgggaaga ccagctctac
     3841 tggaaggcgc aggaggaaca caaagtcaac tacattcgcg gcatcgtgac cgaggtgctg
     3901 cggcgcggcg attccctggt gattaagggc gaagatacca cgatgggacg gcctgtggaa
     3961 ctccccatgg atgtcgtcat cctctccgtc ggcatggtgc cgagcgccgg aaccatcgag
     4021 atcgctaaag tattgaacct gccgcgcgag cgccacggct tcctggaaac cgtcggcgcg
     4081 cccctggata ccgtcaccac accggtaccc ggcatcttcg tggctggcgc ggctgccggc
     4141 ccgaaagaca tcgaggatag catttcgatg gcgggaacgg ctgctatgaa agcggtgagt
     4201 accgtgcgcc gtcatctgcg ccaggtcgcc acaccgctat cagcaggtgg cggaggggag
     4261 tagcgatgcc agctccagca tccaaccacg gcgaaacagt tggccaggtc gttgacacac
     4321 cgagcgcgct cgaatttatg cgcgaggcgc tcgagaaaat caccgaagat gagctggcgg
     4381 ctatcgttgc agagctgcgc gccaaaagcc agcgcgtccg gcaaatgttc gcgctgaata
     4441 tgctcgcgga ttttgacgag agcaagtttc gctctctgct tcgctcgata ttctcgacgc
     4501 gccgacgcgc cgcacaggtc atggcgctcg tcgatgcagg cgccttccgt gatgccgcca
     4561 gcgagctact gcatggaaca gcgccgctgc cagaacgatt cgatgcgttt gtggcgcgaa
     4621 tggagccgct tgacctggcg acgcgctgcg acctggccgg tgagtgcctg cactactggg
     4681 atttcgagcg ctactggctc tggacccgct ggatgtggga ccccgaccgc aacacaggct
     4741 ctctgccact ggtgacaatc caggagtacg atctgcacgg agagactccc gggttgacct
     4801 atgtgaaagt gggggaagct gttgcctatg tgaatggggt gggcgaggcg gccggattcc
     4861 agcacggcgg gcgcgggctg ctcggcacgg atgtctatct cgcctgcgtc tatgtcgtct
     4921 acctgtatac agtgacgcgc atgcgcatga caaaggaatt caataaggtg attcctcaat
     4981 ttccggaact cgctcgccgc ttacttggca cgtataaaat ggagggcatg aaatcatgac
     5041 tgctgtcgaa cccgaacgca ttcacgaaaa aaaggtggca atccaggacg gcgtggacat
     5101 ctccggccac tggaaccgca tgttcgatga gcgcgtgata tgggactacg atacctccaa
     5161 actcgacgag gtcacgcaga tcgaaggggc agagtcgctg ggcgcatgct accagtgcgg
     5221 caagtgtacg cccgtctgcc cggtcgatca cgtgggggac tatagccccc gcaaaatctt
     5281 ccgcaaaacg cagatgggta tcagcctgct tacgagttcg gacctgtggc tctgcaccac
     5341 ctgcgccaac tgcctgcgcg tttgcccaaa agaagtcaat atgattaaga tcatgcctgc
     5401 ggtgcgcgaa caggctgtgc ttgcaggcaa tgctccgacc gagctttcgc aggcattcga
     5461 gaacaccttt cgctacggca atccgctcgg tgagccgacg cgcaaacggg cagactgggc
     5521 cagagatgcc ggtgtgcccg tccccatcct ggcgcagatt cgccgccccg tcgatgtcct
     5581 ctggtttgtc gaatgctatc ccgcctatca cccgcgcggc aaagacgcgg cgcgcggcct
     5641 ggcgcgcatc ttccacgcgc tgggcgttga ctacggcatc ctgggcgtag aagaaaagtg
     5701 cagttgcgac tcgcagcgcc tggccggtga gaagggtctg tttgagatgc acaccgaaca
     5761 caatatcaag atgttcggca aataccagtt caacaccctg gttacggctg atccccacgc
     5821 gctgaacgct ttcaaaaaag agtaccccaa atttggcgga gagtataacg tcctgcacta
     5881 tacacagttc ctggccccca tgctggatcg cattcccttt aagaaaagct tgccgtaccg
     5941 cgtcaccttc catgatccct gctatcttgg tcggcataat ggcgagtatg aagcgccccg
     6001 tcgcctatta gaagcaatcc ctggcattga attaaccgag atggcgcgct gcaaggcgaa
     6061 cggctactgc tgcggcgggg gcggcggcgg catgtggctc gacagcttcg cgcgccctca
     6121 cgtctccgag cgcctttccg aacggcgcgt gcgcgaagcg gtcagcacgg gcgcggatgt
     6181 gctggcggtc tgttgccctt atgaaatctc gcgcttcgag gacgccgtaa aatcgacggg
     6241 caacgacggc aagctgatcg tgcgcgacat catcgaattg atcgacgaat ctatgcgcgg
     6301 ataaactaat agagaaggag taggcgagta tgcatattat cgtcccggtg aagcaagtcc
     6361 cggacctggt cgaagagctg gagatcaaca gcgacggcac agacctcgaa cgggacatgc
     6421 tcaagtacaa gataaacgag tgggatgaaa gcgcgctgga ggaggcgctc cagctcaaag
     6481 agctgcacgg cggcacggtg acggtgctga ccgtggcgag cggcgatgtc gatgtggatg
     6541 agacgctcta tacctgcgtt gcgaaaggcg ccgaccgggt tgcgaagata gtcggcgact
     6601 tcgaggggca ttatgatagc catactgctg ctcgcgccct cgctgaggcc attcgtgaac
     6661 aaccctacga cctgattctc accggggtgc aggccgccga tgacctggat ggacaggtgg
     6721 gagtcttgct ggcaacgtat ctcggcatac cgcatatcag cgtagtcagc ggcatcgaag
     6781 tcgatccact gtcgcgcacg gcaaccgttc accaggagta cgcgggcggc atcatggcga
     6841 ccttcgaggc tgacctgccg cttgtgctgg gcgtgcaggc gtcgcgagcc atcccgcgtt
     6901 acgcgccggt gagccgcgtg cgccaggcta tgaagactgc gcgcatcgag gagatcgagg
     6961 cgacaactcc tgccggggcc ggtatctccg tgcggcgcat gttcaagccg gaatccacgg
     7021 agcacgctga aatgctcgaa ggcgataccg aagaagtcgt tgaccgcgtt atcgagattc
     7081 tccgcgagcg cggactatgg agggcttgaa tatggctgaa catgatgtct tcgtcatcgc
     7141 tgaacacctg gagggcaagc tcagcgatgt gacgttcgag atgctcggca aggcgcgcag
     7201 cctcgcgcca gcgctcggcg ggcgcgcggt cgcggttctg ctcggtcacg aggcgactgc
     7261 gctggccggg caactgggag cggctgacca ggtgttgtac atcgatgatg ccgggctgcg
     7321 ctatttcgtc ccggaacggg ccgagcaagc gttgagaacc gtactctcga atgaaaaacc
     7381 cgccctgacc atgatcgcca acacctctat gggcatggac ctggccgccg cgctatccgc
     7441 cgcgctggac tggcccctga tcgcctattg cagcgacgcg caggcgcaag atgggaagat
     7501 cgtcgccacc agccagattt atggcggcaa gatcgccgtg gaaacccgga gtgaaagcgc
     7561 ctccacgctg gtatccgtcc tcgcaggcgc attcccggcc tccgagggac atgccgacag
     7621 gcccgccgct atccagacca ttttcggcgc tgcgaacgag gggcgtgtgc gctttaaggg
     7681 gctgaccgtt cccgaagccg gcgatgtgga tatcacgcgc gaagctttcc tggtcagtgt
     7741 tggccggggc attggctcga aggacaatat cgaactggtc gaagaactgg caaacaccct
     7801 gggcgcatcg cttgccgcgt ctcgtcccat tattgacagc ggctggctgc caaagcagcg
     7861 gcaggtgggc aaatcgggcc tgaccgtgaa acctaaactg tacatggcgg tgggcatcag
     7921 cggcgcgccg gagcatttac agggtatgag ggatgccgaa ctgatcattg ccatcaacag
     7981 cgatgcgggc gctcccatct tcgaggtagc cgactatggc atcgtgggcg atctcttcga
     8041 tattgtacca gcgcttaccg aacggcttgc cgaggttcgc gcaaaatgac gtaatgggga
     8101 cgaccctcgt ggtcgtcccg gctcctgcgg agaagcgcta tgacgtgggt gagttcaatg
     8161 tcagtcatcc tggccagcag cccgatcggg cctggccggc ccatctttga gtttttcacg
     8221 ccggtcatgg aagtgatctt ttattccctg gccgccatct ccaccctggt gttcctgctg
     8281 ggcgtgttct tgaaggtgcg caaatactgg cgtggcaaat cagagatgcg gctggatcac
     8341 ctgcccaggc gtttaggccg cgccatacgg ctagtggcgc aaaatgccac cgtcggtagg
     8401 cgcgaccgct acgcgggagt cggacacttc atgattatgt gggggttcat caccctcttc
     8461 ctgggcactg tcatcctcgc gctcgactac gacctcattc gccccatcca tcccgactgg
     8521 caattctacc acggcgattt ctacgacatc tacaagctcg tcctggatac gctcggcagc
     8581 gcctttctca tcggcctgct ggccatgatg atccgccgtg gcttctttcg cctgccaaaa
     8641 ctcaactata agcgcgtaga ccgccctgat ggcagctatg atcgccacct ctttaaggta
     8701 ggggacgctc tctttgtggg cggactcttc ctcatcgggc tgacgggcta cctgctcgaa
     8761 ggcgcgcgca tcgcccacga ccagccggtc acgatggcct ggtcgcccgt tggttccctc
     8821 gtctcgcagt tctacctctg gatcggcctg acgccgcctc aactggtcat cagcagggtg
     8881 acgctgtggt ggatacatgt ctccctggcg ctggcgttcg tcgcctacat cccgttttcc
     8941 aaagcggtgc atatgctcgc ggatttcgtc aatctcacct tccgcgacga aaaggccgga
     9001 atcgcattac ctccgatagc ggaggagcca gctccggccc accccggcta ccgcgacatt
     9061 cgcgacttta cctggaagca tttgctcgac ctggatgcct gcaccaagtg cgggcgctgt
     9121 cacgaggcct gccccgcgcg cgcttccggc gcgccgctct cgccccgcga tttgatcctg
     9181 gatttacgag actgtgctga cgaacagctc agtttcaagg ccccgttgag ccagcgcacg
     9241 ctccctgttc tcccggtaga tcacaggcaa aatggacaca acggctcttc tccgcatcac
     9301 aggccggtgg ctggcggggt catcaaggcg gaaacgctgt gggcctgtac cacctgccgc
     9361 gcctgtgtgg aagcctgtcc cgttggtatt gaacacgtgc cgatcatcgt ggagatgcgg
     9421 cgcagcctgc tcgacgaagg cgagatggac ccgaaactcc aggaagccct caccaatctg
     9481 tcgaaatacg gcaattcctt tggcgtctcg gatcgcctga gggcaaagtg gaccaaaact
     9541 ctgcctttca agatcaagga cgctcgcaag gaaccggtcg aatatctctg gttcgtgggc
     9601 gattttgcct ctttcgatca gcgcgtgcaa gacatcacgc gcaaggtggc aactgtcttc
     9661 cagcaggtcg cgctggattt cggcatcctg tacgaaggcg agcgcaattc tggtaacgat
     9721 gtgcggcgcg ttggagaaga gggcctgttc cagatgctgg ccgagcagaa cataaagtct
     9781 ctgggaaagg ccactttcaa aaccatcgtg acaactgacc cgcatagtct gaacgctcta
     9841 cgcaac
//
LOCUS       DASZAK010000987         1849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_487125, whole genome shotgun sequence.
ACCESSION   DASZAK010000987 DASZAK010000000
VERSION     DASZAK010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487125"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VGT44_17295"
     CDS             <1..585
                     /locus_tag="VGT44_17295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582617.1"
                     /translation="GSRPVLPPIPGADLPHVATVWQVLQGEKDAQPGENVLVFDQIGF
                     HQATSTAEFLADLGCQVEIVTGQFYVGGDLGITLDLELWYRRALAKGIRLTPNHFLAG
                     IGPDSATIINNYTGQARQIESLALVVMATQQAANDELYFQLHGKVKELYRVGDCVAPR
                     RVEHAILDGERAARALGSASNQRLEPGKELHPTF"
     gene            complement(594..1679)
                     /locus_tag="VGT44_17300"
     CDS             complement(594..1679)
                     /locus_tag="VGT44_17300"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV2582618.1"
                     /translation="MTASHQIPIIVGHQDTLLRLSLAGREKGRSFFERGEQGHLDLPR
                     AREGGVAGSFFAIFVPAPPETIKVGVGRVGMNVIQTDQGFEVPYAPAIEMDYARRVTM
                     AMMATLFRLEDESDGQLGVVRSTDELIYCLDNDIHAAILHFEGAEAIDADLDALEVFY
                     RAGLRSLGLVWSRPNIFAQGVPFKFPHSPDTGPGLTEAGRELVRGCNRLGIMLDLSHL
                     NEQGFWDVARLSEAPLVATHCNAHALSPSTRNLTDRQLDAIKESGGIVGLNFAVYDLR
                     DDYEKDPNTPLELLVRHIDHLVSHLGIDGVALGTDFDGTTVPAAIGDAAGMPNLVEAL
                     RMHGYDDAALRKIGYENWLRVLQQTWK"
BASE COUNT          392 a          571 c          548 g          338 t
ORIGIN      
        1 ggatcgcgtc cagtgctacc acccattcca ggcgccgatc tgccacacgt cgccacagtc
       61 tggcaagtcc tacaaggtga gaaagacgcg caaccgggag aaaacgtcct cgttttcgac
      121 cagatcggct tccaccaggc caccagcacc gccgaattcc tggccgacct gggctgccag
      181 gtcgagattg tgacgggtca gttctacgtc ggaggcgacc tgggcatcac gctcgacctc
      241 gaactgtggt accgccgcgc gctggcaaag ggcatacgcc tgacgcccaa ccatttcctg
      301 gcgggcatcg ggccggacag cgcaaccatc atcaacaact acaccggcca ggcgcgccag
      361 attgagtccc tcgcgctggt ggtgatggcg acgcagcagg ccgcgaacga cgaactatac
      421 ttccaactgc atggcaaagt gaaggagcta taccgcgtgg gcgactgcgt ggctccgcgc
      481 cgcgtggaac atgccatcct ggacggggag cgcgcggcgc gagcgttagg aagcgcatcc
      541 aaccaacgct tggagcctgg gaaggaattg catccaacct tctaaatgat ggttcatttc
      601 catgtttgtt gtagcacgcg aagccagttc tcatacccga ttttgcgcag ggcagcatcg
      661 tcatacccat gcattctcaa ggcttcaacc aggttcggca tcccggcggc gtcgccgatg
      721 gcggcgggga cggtggtgcc gtcgaagtcg gtgccgagcg cgacgccgtc gatgccaaga
      781 tgcgaaacga gatgatcgat gtgacgtacc aacagttcaa gcggggtatt gggatctttc
      841 tcataatcgt cgcgcaggtc gtagacggcg aaatttagac ccacgatgcc gcctgattcc
      901 ttgatggcgt cgagctgccg gtcggtcagg ttgcgcgtcg aagggctgag cgcgtgggca
      961 ttgcagtgtg tggccaccag gggcgcctct gacaggcgcg cgacgtccca gaagccctgc
     1021 tcgttcaggt gggagaggtc gagcatgatg cccaggcggt tgcagccgcg caccagttct
     1081 cgcccggcct cggtcagccc tggcccggta tcaggcgagt gcgggaactt gaagggaacc
     1141 ccctgcgcga agatgttcgg acggctccaa accagcccca gcgaacgcag cccggcccga
     1201 taaaagactt cgagcgcgtc caggtctgca tcaatggcct ccgcgccctc gaagtgcagg
     1261 atggcagcgt gaatatcgtt gtcgaggcaa taaatcagct cgtccgtgct gcgtacaacg
     1321 cctaactgcc catccgactc gtcttccagg cgaaaaagcg tcgccatcat cgccattgtc
     1381 acgcgccgcg cgtaatccat ctcgatggcc ggggcatagg gaacctcaaa gccctgatcg
     1441 gtttgaataa cgttcatgcc cacgcgacca acgcccacct tgatcgtttc gggcggagca
     1501 ggcacgaaga tggcgaaaaa gctgcctgct accccaccct cgcgagcgcg cggcagatcg
     1561 agatgcccct gctcgccgcg ctcgaaaaac gaacgccctt tttcgcggcc ggcaagagag
     1621 agcctcagca gcgtatcctg gtggccaacg ataatgggga tttgatgcga agcggtcatg
     1681 taaggtcctc tttcatgctg tgattaacca ctcaacagcg taccccattc ttagcgcaag
     1741 cgcaataggg ggttccaacg catacatgaa cttttacaac caaagtcggt cctgtcatcc
     1801 tgagcgcagc gaaggatctc atgacccatt gaccgagatc cttcgctgc
//
LOCUS       DASZAK010000988         5095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_201148, whole genome shotgun sequence.
ACCESSION   DASZAK010000988 DASZAK010000000
VERSION     DASZAK010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201148"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VGT44_17305"
     CDS             <1..622
                     /locus_tag="VGT44_17305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582619.1"
                     /translation="TFPNVCPAENAFTAAAVNVTSMDGALIPGYNRTPMNDFPRIAVS
                     DKNGTVSIVWNDARYHAVGDILLQSFNLDGSFPTPVQATPVRINSAIGGWHMLPALRN
                     TDDDGDLQISFYGRASANTDVTNVYAAIDVSSLATITPGNVKVTTASSAWNSVSSDIV
                     PNFGDYTDNYMQAQSVVPYTTQTLYVAWSDGRLGVPQPFNAHAHTA"
     gene            2011..3555
                     /locus_tag="VGT44_17310"
     CDS             2011..3555
                     /locus_tag="VGT44_17310"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEV2582620.1"
                     /translation="MNTALLIARLLLAAVFLVAGFGKLADLAGSRQALRDFGVPAVLA
                     NPFGVLLPLAELAVAVALIPLAFAWWGALGALALLLLFVAGIGYNLTRGRTPDCHCFG
                     QIHSAPAGWPTLIRNVILAAIAGFIVWQGRTNAGTDIGTWFGTLAVAQRVELIVGTII
                     VALIALEAWALFQGLSQQGRLLLRIEALENRLANVGRTGQPSADTVFRGLPVNTQAPN
                     FSLSGLHGETLTLDALRAQDKPVVLVFSDPGCGPCSALLPEVGRWQRDYAGKLTLAII
                     GRGAPEENRAKSAEHGITQLLLQKESEVAESYQVAGTPSAVLIRTDGTIATPLAEGAD
                     DIRGLVAGAIGLPVLKSLPLAAGMQSNGNGGAAMANQPAGQQVGKQAPDFSLPDLSDR
                     TVKLSDFRGEKTLVLFWNPGCGFCQRMLDDLKAWEEKPSEGAPKLLVVSTGSVENNKA
                     LGLRSPVLLDQGFSVASRYGANGTPMGVIIDEGGKIASEVAAGALDVLSLARSGNASA
                     TPMATM"
     gene            4038..4787
                     /locus_tag="VGT44_17315"
     CDS             4038..4787
                     /locus_tag="VGT44_17315"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEV2582621.1"
                     /translation="MRKPTGIVAALLILFLLASCASTTITQQPAGGSSPASQATPTHQ
                     SSQPSGTFTHAITYVALGASDAVGVGSDTPGAQGYVPLIAAHLPKGSHMLDLGISGIH
                     LHEALSEELPLALSTSPDLVTIWLVANDFVGGVTYNDYMQDLVTLLSQLRSHTHARIV
                     MADLPDLTRLPAFANQTAAQKSQMLAGIQQWNRGIAQQAARYAVTLVDLFSQGSTLTA
                     HPEYISGDGFHPSPAGYIQLANLFWKAIQQQ"
     gene            4893..>5095
                     /locus_tag="VGT44_17320"
     CDS             4893..>5095
                     /locus_tag="VGT44_17320"
                     /inference="COORDINATES: protein motif:HMM:NF039728.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HEV2582622.1"
                     /translation="MAIGPLEFVVIGCKGHQFSKEILPELNSIQEKGLFRVVDLFFVR
                     KDNKGAISVLEVSDLNDEELAAF"
BASE COUNT         1070 a         1458 c         1442 g         1125 t
ORIGIN      
        1 aaccttcccc aatgtttgtc ccgcggagaa tgcattcacg gcggcggcag tcaacgtcac
       61 ctcgatggat ggcgcgctca tcccgggcta taaccgcacc ccgatgaacg acttcccgcg
      121 cattgcggtg agcgataaaa acgggacggt gagcattgtc tggaacgatg cccgttacca
      181 cgcggtaggc gatatcctgc tgcagagctt caatctagac ggtagcttcc cgacgcctgt
      241 ccaggcaacg ccggtgcgca tcaacagcgc tatcggcggg tggcacatgc tgcctgccct
      301 acgcaacacg gatgacgacg gcgacctgca gatctcgttc tacggtcgcg cctcggcgaa
      361 caccgatgtg acgaacgtgt atgctgccat cgatgtcagt tctctcgcga ctattacgcc
      421 cggcaacgtc aaggtgacga cggcttcgag cgcctggaac agcgtttcat cggacatcgt
      481 cccgaacttc ggcgactaca ccgataacta tatgcaggcg cagtcggttg ttccttacac
      541 tacacaaacg ttgtatgtgg cgtggtctga tgggcgcctg ggtgttccac agcccttcaa
      601 tgcccacgcg cataccgcct gaccagttcg cggagcggtc aaccgggttg gcatttgcca
      661 gtacatgcca atccggatcg gcggctcagg ttcgaagcaa cgggggcagt ctcgattctg
      721 tgatttacta cgcaactttc cctggcgatc atcattgatc aaaccctata ttttgtgttt
      781 gcgtgtaact cgttcatctc gcgacctgtg cggggcacga atgaaggctc ctcgcataat
      841 gtgactttag tgggttacac agggtaacaa aaggagcgaa gggagatgga accctctcaa
      901 tttgataagt ttaccaaatc gctggcgaca ccgacttcac gtcgcagggc tctcaaggct
      961 ctggtagcca gtgccgctgg cagcatcttt gctttcagtg gaatcggtcg ggtcttcgcc
     1021 tcgaggaaat gtccacccgg tctgacgaac tgcagtggca agtgcgtgaa cctgaacaat
     1081 gatcctaagc actgcggtga ttgcgatgtc aaatgcgcat caggcctgtg cgtcaacggg
     1141 ctgtgctgcc cgcctggggc agtccagtgc ggcaattcct gctgtagctt tacctgctgc
     1201 ggcggtaaca cttgcgtgga catcaacaac gataagaaca actgtggtgg gtgcggcaat
     1261 gtctgcccgt ctacagcgac ctgtcataac gggcagtgtc tctgcccaac ggggaatccg
     1321 ccttgcggca gtacctgctg tacaactgga caggtttgcc tcaacggtaa tacgtgctgc
     1381 gacacttcgc aagtgtgcgg caatacgtgc tgcccggctg gacaggtttg ccagggaggc
     1441 atatgcgtta cacctatatg ttcaggaggg aactgctcta aaggattcag caattgtggg
     1501 accaatccga attgcttctg ctttacggac cctactggag ctggacaatg tggctgcaac
     1561 agcttctgtg ctggtttaac gccttgcaca tcgaactcca attgcccagt tgggttcttc
     1621 tgtgcggtcg gaactggttg taattgtagt ggcggagtat gtatacggaa ctgtactcaa
     1681 acgtgtacac tcagtccttc acaaggagga ggtccaaccg ctgcccatcc ctaagcggtt
     1741 tgaatgacga actgccagga tcgataccag gtgtggtccc cgcacctggt atcgacctcg
     1801 ctcaggagac cgtactctca ccagcatggt ctcctggagt agttccgatg gcgtaagtga
     1861 aacgtggcct ggcgatccct cgcggtcgcc atgttgaaga attttaaata gccctatatt
     1921 ctttttcttt ggcatttcac ctactgttga ggtagacttg tttcggagga agtagcgggt
     1981 gctttccaat ggtaatgagg agaaacgctt atgaatactg ctcttttgat tgcacgcttg
     2041 ttgcttgctg cagtcttcct cgtagcgggg tttggcaagc tggctgacct ggccgggtcg
     2101 cggcaagcac tgcgcgactt cggcgttccc gcagtacttg ccaatccctt tggtgtgctg
     2161 ctgccgctgg ccgagcttgc ggtagcggtg gcgctcattc cactggcctt tgcctggtgg
     2221 ggggcgcttg gcgctctggc cctgctgctg ctctttgtgg caggcattgg ctacaacctg
     2281 actcgcgggc gcacgcccga ttgccactgt ttcggccaga ttcattctgc tcccgccgga
     2341 tggcccacgc taattcgcaa cgttatcctg gctgccattg cgggtttcat cgtctggcag
     2401 ggacggacca atgcagggac ggacatcggg acctggtttg gtacactggc ggttgcgcag
     2461 cgggtcgaac tcatcgttgg gacaatcata gtcgcgctga tcgcgcttga ggcctgggcg
     2521 ctctttcagg gactgtcgca acaaggacgg ctgctgctgc gcatcgaggc tttagaaaat
     2581 cggcttgcca atgtgggcag gacagggcag ccgtcggcag atacagtttt caggggactc
     2641 cctgtcaaca ctcaggcacc caattttagt ctttctggcc tgcatggaga gacgctcacg
     2701 ctggatgcgc tgcgcgccca ggacaagccg gtggtactgg tcttctccga tccaggctgt
     2761 ggcccttgtt cagccctctt gccagaggtt ggacgctggc agcgcgatta tgccggcaag
     2821 cttactctgg cgatcatcgg tcgcggcgcc cctgaggaga atcgtgccaa atctgccgag
     2881 catggcatca cacagctatt gctgcagaaa gagagcgagg tggccgaatc ctaccaggtg
     2941 gccggtaccc ccagtgcggt actcatccgt accgatggga cgattgccac tccccttgcc
     3001 gagggcgcgg atgacattcg tgggctggta gcgggcgcaa taggtctgcc agtgctcaaa
     3061 tccttgccgt tagctgctgg tatgcagagc aacggcaatg gtggagcggc catggcaaat
     3121 caacctgctg gacagcaagt aggcaaacag gccccggatt tcagcttgcc ggacctcagt
     3181 gaccggactg tgaagctatc cgacttccgt ggcgagaaga cgctcgtgct cttctggaat
     3241 ccgggctgcg gtttctgcca gcggatgctg gatgatctga aagcctggga ggagaagcca
     3301 tccgaagggg caccgaagct gctcgtggtc tcgaccggca gcgtggagaa taataaggcg
     3361 ttgggactgc gctcgccggt gctgctcgac cagggcttca gcgtcgcctc ccgatatggt
     3421 gccaacggca cgcctatggg agtgatcatc gatgaaggag ggaagattgc ttccgaggtt
     3481 gcggcggggg ccctcgatgt actctccctg gctcgctcag gtaatgcctc ggcgacgcca
     3541 atggccacca tgtaggaaaa gaaaaaacct cgacgatcgc ttgtttcgag gggcgtaatc
     3601 gtgtgcgcta gatttgagcg aatgaacagg gacgcgggcg tcgcccgcgt ccccctctca
     3661 acacaaccct gctcctgccc agggagaatt gtctcgacgt gctgggtcgc attttgatcc
     3721 acacatggtt gggcagtgcc gctccttcta tatcctacct ggcagagatg tgagggaaca
     3781 cttcctaatt ccgcgacccc ttgccagtac gaacccccaa cgatgatagc aagtacacct
     3841 tcagcgggaa gcgcgtcgag cgtggcctct atcgatcatc ggacaatcgg tatatcaaag
     3901 ctgatgtcaa tggtgcgtat aatataatac gaaaagtagt acctgatgcc tgcggggcgg
     3961 tcaatagaac gctatgtttt tgggaactat aaaagggaat aatctgaaca tgaaggggaa
     4021 aagagaggag catccatttg cgtaaaccga ccggcattgt ggcggcgtta ctgatcctat
     4081 tccttctcgc gagctgcgca tctactacta tcacccaaca accagcaggt ggctcatctc
     4141 ctgcatcaca ggcaacccca acccatcaat cttcccaacc gtccggcaca ttcacccacg
     4201 ccatcaccta cgtcgcactg ggcgcgtccg acgccgtggg cgtcggcagc gatacccccg
     4261 gcgcgcaggg ctacgtcccc ctgattgccg cgcatctccc caaaggctcg catatgctcg
     4321 acctgggcat cagcggtatc catctgcacg aggcgttgag cgaggaactg ccgctggcgc
     4381 tctcaacctc gcccgacctg gtgacgatct ggctggtcgc caacgacttc gtcggcggtg
     4441 tcacctacaa tgactatatg caagacctcg tcactctgct ctcccagctg cgctcacaca
     4501 cccatgcccg catcgtcatg gcggacctac ccgacctgac gcgcctgccc gccttcgcta
     4561 accagaccgc cgcgcagaag tcgcagatgc tcgctggtat ccagcaatgg aacaggggca
     4621 tcgcgcagca agcggcgcgc tacgcagtga cgctggttga cctgttcagc caggggagta
     4681 cgctgaccgc ccaccccgaa tacatcagtg gcgacggctt ccaccccagt cccgccggat
     4741 atatacaact tgcaaacctg ttctggaagg cgattcagca acaatgaacc ccctcatttc
     4801 gtaaatattg tatgatgctt cacgtgactg tcccaattta catgtgaaac ccagaggcca
     4861 gcatcgctaa acataaacaa aggagtgaga atatggccat cggcccactt gaattcgtcg
     4921 ttatcggttg caaaggccac caattctcga aagagatctt gccagaatta aactccatcc
     4981 aggagaaagg tctcttccgc gtagttgacc ttttcttcgt caggaaagat aacaaaggcg
     5041 ctatctcagt cctagaagtg agcgacctga acgacgaaga gctggcagct tttga
//
LOCUS       DASZAK010000989         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_381844, whole genome shotgun sequence.
ACCESSION   DASZAK010000989 DASZAK010000000
VERSION     DASZAK010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381844"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..2004)
                     /gene="htpG"
                     /locus_tag="VGT44_17325"
     CDS             complement(103..2004)
                     /gene="htpG"
                     /locus_tag="VGT44_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEV2582623.1"
                     /translation="MTVDTHKETMGFQAEVKQLLDLMIHSLYSNKEIFLRELISNASD
                     AIDRLRFAALSDAALFEDDTDMAIHVVFDKDARTITVSDNGIGMSRSEVIEHIGTIAR
                     SGTREFFQSLTGDQQKDASIIGQFGVGFYSSFVVADRVTLTTRRAGEPADAGVRWESD
                     GQGEYTVEAVEKPSRGTEVTLHLRSDEDELLSGYSLRSIIKKFSDHITIPILMKVEGK
                     DEKESDEEERVNSASALWTRSKSEISQEEYNEFYKHVSHDFADPLAHLHARMEGKQEY
                     TLLLFIPATAPFDLWNRERRHGVKLYVRRVFIMDDAEQLMPGYLRFVRGIVDSNDLPL
                     NISREILQQNRLIETIRSNAVKKVLGLLADLAAKESEKYEKFWNEFGRVLKEGMAEDH
                     ANRDALAKLLRFASTTSGSEKQDVSLESYVARMKEGQDKIYYMIADSYAGAAKSPLLE
                     IFKKKGVEVLLLSDPVDHLLTAELDVFDGRQLQSISRGEVDLEKIQDVEEQAADGVQQ
                     AGREASDVNALVGRLKSALDERVKDVRVTSRLTSSPACLVVDDYDMDPNLLRLLKAAG
                     QQVPTMKPILEINPRHPIIVRISNESDEQKFADWAAVLFDQSVLTLGEQLEDPISFVN
                     RLNALLSQL"
     gene            2232..>2812
                     /locus_tag="VGT44_17330"
     CDS             2232..>2812
                     /locus_tag="VGT44_17330"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2582624.1"
                     /translation="MTTTPHQQQSSTTSAHAPLQLAQSKQDMASSPTLAMNEAVAARK
                     AAGQSIIHLGFGEASFPLHPLLAETLTRAARSTGYAPVLGLPALREAIAAYLARTRGL
                     TYSPANIAVAPGSKPLLFALIQLLEGDVLLPAPSWVSYAPQARLANRRVTLVETDQRD
                     HHRLTTGALADALTRAQHDGTSPRILVVNTPSNP"
BASE COUNT          576 a          935 c          706 g          595 t
ORIGIN      
        1 aattcattgc gccctcgttc cggatcacgc gactccacct gggcgcgatg aattgcgccc
       61 ctacacgccc attgaccttt gacaaaacac tacgaccatt tatcacaatt gcgacagcag
      121 cgcgttcagc ctgttcacga agctgatcgg gtcttccaac tgctcgccca gcgtcagcac
      181 cgattgatcg aacagcactg ccgcccaatc ggcaaacttc tgctcatcgc tttcgttgct
      241 gatcctgacg atgatggggt gacgcgggtt gatctccagg atcggcttca tcgtggggac
      301 ttgctgaccc gccgccttca gcaggcgcag caggttgggg tccatgtcat agtcgtccac
      361 caccaggcag gcaggcgaac tcgtcaggcg cgaggtgacg cgcacatcct tgacccgctc
      421 gtctagcgcg ctcttgagtc gtcccaccag ggcattgaca tcgctggctt cccgccccgc
      481 ctgctgcacg ccgtcagcag cttgctcctc aacgtcctga atcttctcaa gatcgacttc
      541 gccgcgcgag atggattgca actgcctgcc atcgaacaca tccagttccg ccgtcagaag
      601 gtgatcgacc gggtcagaga gcaacagcac ctcgacaccc ttcttcttga agatttcgag
      661 cagtggactc ttcgccgctc ccgcgtagct gtccgcgatc atgtagtaaa tcttatcctg
      721 cccctctttc atgcgcgcga catagctttc cagcgaaaca tcctgcttct cgctgccaga
      781 ggtggttgag gcgaaacgca gcagcttcgc cagcgcgtcc cggttggcgt gatcttccgc
      841 catgccttcc ttcagcaccc gcccgaactc gttccagaat ttctcgtact tctccgactc
      901 cttcgccgcc aggtccgcca gcaggccaag taccttcttg acggcattgg agcggatcgt
      961 ctcgatcagg cggttctgct gcaagatttc gcgcgaaatg ttgagcggca ggtcgttaga
     1021 atcgacgatg ccgcgcacga aacgcaggta gcccggcatc aactgttcag cgtcgtccat
     1081 gatgaagacg cgccgcacgt acagcttgac gccatgccgc cgctcgcgat tccacaggtc
     1141 gaacggcgcg gtcgcaggaa tgaagagcag cagagtgtat tcctgcttcc cttccatgcg
     1201 tgcgtggagg tgtgccagtg gatcggcgaa atcgtgcgag acgtgcttgt aaaattcgtt
     1261 gtactcttcc tggctgatct cgcttttcga gcgcgtccac aaagccgatg cgctattgac
     1321 ccgctcctct tcatccgact ccttctcgtc cttcccctcc accttcatca ggatagggat
     1381 ggtaatatga tcggaaaatt tcttgatgat cgaacgcaga ctataaccgc tcaacagttc
     1441 atcttcgtcg gaacgcaggt gcagcgtcac ctcggttccg cgcgacggct tttcgacagc
     1501 ctccaccgta tattcgccct gtccgtcgga ttcccagcgc acacccgcgt cggcaggttc
     1561 cccggcgcgc cgcgtcgtta gcgtcactct atccgccacc acgaacgagg agtagaagcc
     1621 gacgccaaac tgcccgataa tgctggcatc cttctgctgg tcgcccgtca gcgactggaa
     1681 aaactcgcgc gtgccagagc gggcaatcgt gccgatatgc tcgatcacct cgctgcgcga
     1741 catgccaatg ccgttatcgc tgacagtgat ggtgcgcgca tccttatcga acaccacgtg
     1801 aatcgccata tccgtgtcat cttcaaacag cgcggcgtcc gacaacgccg cgaagcgcag
     1861 ccgatcaatc gcatccgagg catttgagat cagctcccgt aaaaaaatct ccttgttgct
     1921 atacaaggaa tgaatcatca aatccagcag ttgcttgacc tctgcctgga agcccatcgt
     1981 ctctttgtgt gtatccaccg tcatatgaac cagactcctt tattccaatt ccatcaaatt
     2041 cacgccatac tacaggcacc tggggtgtgc tacggtatat aatgtatcaa aatagtttcg
     2101 tgattacaag agtctttatt ccgatgcaac agatcctaat cctcgtccct ttggcaattg
     2161 tcattctagc actcgttcaa gaacagcgaa aacgcgctca gaaataatcc gggaaaggac
     2221 ttgtactcca tatgactacc acacctcatc agcaacaatc aagcacaaca tctgcccatg
     2281 cgccgctgca attagcccaa tcgaaacagg acatggcaag ctctcccacg ctggccatga
     2341 acgaggcggt cgcggctcgc aaggccgccg ggcagagcat cattcatctg ggcttcggcg
     2401 aggcatcgtt tcccctgcat cccttgctgg ccgagacgct gacccgcgcc gcgcgcagca
     2461 ccggctacgc tcccgttctc ggcctgcccg cattgcgaga agccatcgcc gcatacctgg
     2521 cgcgtacgcg cgggctgact tactcgcccg cgaacatcgc cgtcgcccct ggcagcaagc
     2581 cgctgctatt cgcgctgata caactgctgg aaggcgacgt gctgctgccc gcaccctcct
     2641 gggtcagcta cgccccgcaa gcgcgcctgg ccaaccgccg cgtcacgctg gtcgagaccg
     2701 accagcgcga ccatcaccgc ctcacaaccg gcgcgctggc cgatgccctg acccgcgcgc
     2761 aacacgacgg aaccagtccg cgcatcctgg tcgtcaatac gccgagcaat cc
//
LOCUS       DASZAK010000990         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_631139, whole genome shotgun sequence.
ACCESSION   DASZAK010000990 DASZAK010000000
VERSION     DASZAK010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631139"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1375)
                     /locus_tag="VGT44_17335"
     CDS             complement(8..1375)
                     /locus_tag="VGT44_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2582625.1"
                     /translation="MRRLFATLAPVCIIFVLVLAACGSSNNGNGSGNNGVNQQGLAGG
                     NSYIGCPSNANTTAAAPETGNVTLTVSGWTSTPAEDGLVQQNLQNFEKLHPNIKINWS
                     PIPGDYPTKMRANVASNTVPDVFYLQPAMSSEYITSGKLLNLSPYMAHDNVQASDYYS
                     ALLNPFVCTSGQVYGLPKDWNSLGVFYNKQMFQAAGLSAPSANWTWSDMQHDAQVLTK
                     NAGSPNSVYGVVLSADLSRWGAFLLEDGGSVLSKDGTQATFNNQNGINALQFYDSFFK
                     NNTGALPTTVGAPWNGDAFGKQRAAMVIEGGWLIPYMASTYPSVQYGIAPMPTAPSGK
                     QADLTFTNAWSAYSGTQHPEAAWELIKYMTGSTVQESQLNAGFALPTLKSLANAPYFT
                     SNPGFKVLFDAAPYSFADYYGSQDSTIHTDLSNAIDAVLLNKQDPQSALNDAATKINS
                     QLQGG"
     gene            1626..2168
                     /locus_tag="VGT44_17340"
     CDS             1626..2168
                     /locus_tag="VGT44_17340"
                     /inference="COORDINATES: protein motif:HMM:NF039822.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5666 domain-containing protein"
                     /protein_id="HEV2582626.1"
                     /translation="MEENDQMASAPLNQPLAPRRRGRRWLLLGGLAVAFMFALAVGVF
                     LGSSTGSTQAAALTSGSSNSVQTLAAAQNDTANSQPPRNSSGNGFAQQPNFGSQAPGA
                     QGPQGQCESLTVSSASGSTIVAKTASGSTVTVHTTASTKYTQAGKTVAASAIKPGVRI
                     SVMGTHNSDGSITATSIVIG"
     gene            complement(2470..3252)
                     /locus_tag="VGT44_17345"
     CDS             complement(2470..3252)
                     /locus_tag="VGT44_17345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2582627.1"
                     /translation="MEKPLHRILIAEDEEIARRALCRVCEQSGCPVEVVFQAATGRQL
                     LEALSKVQPDIILMDVVMPGMDGLAATRAAHELYPSTRVVIISAHDRFEYAQEALRAG
                     AVDYLLKPVRAEQLVTLLKKLCAELDAENTNKMSAAALQEEKVEVQQARSPHYATMKR
                     AEAYIAENFARSLTLEQVASHVDMAPTYFSRIFKQEQGCTFIAYLTRVRLEQARRLLR
                     TTTLSVAKIGHAVGYQGPNYLAEVFKTSEGITPGAYRRNHSG"
BASE COUNT          671 a          923 c         1016 g          759 t
ORIGIN      
        1 ctacgggcta tccgccttgc agctgggaat taatttttgt agccgcgtcg ttcaaggcgg
       61 attgcgggtc ctgtttgttg agtaagaccg cgtctatggc gttactgagg tccgtgtgga
      121 tcgtggaatc ctgggagcca tagtagtccg cgaaactgta gggtgcggca tcgaacagca
      181 ccttgaagcc tggattcgac gtgaagtagg gcgcattggc caggcttttc agcgtcggca
      241 gagcgaagcc cgcgttgagc tggctttcct ggacggtcga gccggtcatg tacttgatca
      301 gctcccacgc cgcttctgga tgctgggtgc cggagtaggc tgaccaggca ttggtgaagg
      361 ttagatcggc ctgcttcccg ctgggggctg tcggcatggg cgcgatacca tactggacgc
      421 ttggataggt ggatgccatg tagggaatga gccagccgcc ttcgatcacc atcgcggcgc
      481 gctgtttgcc gaaggcatcg ccgttccacg gcgcgcccac cgttgtcggc agcgctccgg
      541 tattgttctt gaagaaactg tcgtagaact gcagcgcatt gatgccgttc tggttattga
      601 aggttgcctg cgtgccatcc ttgctgagga cggagccgcc atcctccagc aggaacgcgc
      661 cccagcgtga aaggtcggcg gagagcacga cgccgtagac cgaattgggc gagccagcat
      721 tcttggtcag cacctgggcg tcgtgctgca tgtcgctcca ggtccagttg gcgctgggcg
      781 cggacagccc ggcggcctgg aacatctgct tgttgtagaa gacccccaga ctattccagt
      841 ccttcggcag accgtagacc tgcccgctgg tgcagacgaa cgggttgagc agcgcggaat
      901 agtaatcgct ggcctgcacg ttgtcgtgcg ccatgtaggg cgacaggttc agcaacttgc
      961 cggaggtgat atactcgctg gacatggcgg gctgcaagta aaagacatca ggaacggtgt
     1021 tgctggcgac attggcgcgc atcttggtgg gataatcacc aggaatcggc gaccagttga
     1081 tcttgatgtt cggatgcagc ttctcgaagt tttgcaggtt ctgctggaca agcccatcct
     1141 cggcgggggt cgaagtccag ccggagaccg tcagggtgac gttgccggtt tcaggcgcgg
     1201 cggccgtcgt gttggcgttg ctggggcagc cgatgtagct attcccaccg gccagccctt
     1261 gctggttgac gccgttattg ccgctcccgt ttccattgtt acttgagcca caggcggcta
     1321 aaacaagtac gaagataatg catacaggtg ccagcgttgc aaagagtcgt ctcataacga
     1381 acctccctgt tggatgtcta tgcttctttg accaacaaat acacaattca atgcaaagca
     1441 gcggcgtttc cgttcaaagt gaagctgctg aaatgcctta tagttagaca cgtatcacgc
     1501 gggttatccg atacagcatg agagaaatca catgttgttc caattggttt cacatccctc
     1561 caacttatac tcaaattgcg acggcgcgat ctgtcgctaa tttcacaagg aaaggaggga
     1621 aatgcatgga agagaatgat cagatggcat ccgctcccct gaaccagcct cttgctccgc
     1681 gtcggcgcgg gcgcaggtgg ctgttgctgg gcgggctggc ggttgctttc atgtttgctc
     1741 tggctgtcgg cgtcttcctg ggttcgtcaa ccggctctac ccaggccgcc gcgcttacct
     1801 ctggaagctc caacagcgtc cagaccctgg cggcagcgca gaacgacacc gcgaactccc
     1861 agccgccgcg caacagctct ggcaacggct tcgcgcagca gccaaacttt ggttcgcaag
     1921 cgccaggcgc gcaagggccg cagggacagt gcgagtcgct gaccgtgtcg agcgcgagcg
     1981 gtagcacgat cgtggcgaaa accgcgagtg gcagcacagt caccgtccac acgactgcga
     2041 gtacaaagta cacacaggcc gggaagacgg ttgccgcgag cgctatcaaa ccgggcgtcc
     2101 gcattagcgt catggggacg cataatagcg acggcagcat caccgccacg agcatcgtca
     2161 tcggttagta gtatgttaca gcgcaccggc aacggggtca gtgcgatgcg cctggttgga
     2221 ggatgcgcaa ctcaactctg gtgagttcag gcaatgctgg attcgccagg gagcaatcat
     2281 taataagcat cttttacaat aatttcacac ttttcccata gaaccttgag gattttaagc
     2341 taagatacta atgtgatatg cgagacgaca acagagacat cctttcactt tttcttcctc
     2401 tcccccagcg aagaggggtg gcgcggcaca ggccgtgtca cccctcttca gctttttggc
     2461 aactattttt cagccactat gattgcgcct gtacgcgccg ggcgtgatgc cctcggatgt
     2521 cttgaacacc tcggcgaggt agttgggacc ctggtagccg acagcatggc cgatcttcgc
     2581 taccgataac gtggtggtgc gcagtaaacg cctggcttgc tccaggcgga cgcgagtcag
     2641 gtatgcgatg aaggtgcagc cctgctcttg tttgaagatg cggctgaaat aggtgggagc
     2701 catatcgacg tgcgaggcga cctgctcgag cgtcaggctg cgcgcgaagt tctccgcgat
     2761 gtaggcttca gctcgtttca tcgtcgcgta gtgcgggctt ctcgcctgct ggacttcgac
     2821 cttctcttct tgaagagcgg cggcgctcat cttattcgtg ttttcggcgt ccaactcggc
     2881 gcacaatttt ttgagcagcg tcacgagttg ctcggcacgt accggcttga gcagataatc
     2941 gactgccccg gctcgcagcg cctcctgggc atattcgaag cggtcatgcg cggagattat
     3001 tacgaccctg gtggatggat agagttcgtg cgctgccctg gtcgcggcca gcccgtccat
     3061 gcccggcatc acgacatcca tcaggatgat gtcgggctga accttgctga gcgcttcgag
     3121 gagttgccgc ccggtcgccg cctgaaaaac gacctctacc ggacagccgc tctgttcaca
     3181 gacgcggcac aatgcccttc gggcaatctc ttcatcttcc gcgatgagaa tgcgatgtaa
     3241 tggtttctcc actagtttcg ccttttgcgt gaatgtcgtg ctttatgggt agtaggctgt
     3301 caagcttgag gtgtcgagta ggggcgcaat tatgaagatt acgtattaaa gcgggtatac
     3361 accgttaat
//
LOCUS       DASZAK010000991         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_631153, whole genome shotgun sequence.
ACCESSION   DASZAK010000991 DASZAK010000000
VERSION     DASZAK010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_631153"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VGT44_17350"
     CDS             <1..829
                     /locus_tag="VGT44_17350"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV2582628.1"
                     /translation="AALLDADLSGANLTGTDLSEAVLNRSRLIGANLSAATLRDADLQ
                     DATLRGANLSGANLQGANLRYTVLADADLSGADLTGCAIYGVSAWNTRLHGAIQARLD
                     LTDSPAPGHPVSQPAITVGHLAAAQLLHLLSQNAYTPAVHSALSRHIVLILGRFPPER
                     QQTLQAIRHDLWERNYVPVFVDVLALEEQAHAGLIDVLIGMASGVLVDLSDAPYAEVM
                     FPRRPIPVQPVLVEGQVAREVFPLFYHQLLPLSRYESTEGLRRCLAVFLDRLSTATH"
     gene            2567..3001
                     /locus_tag="VGT44_17355"
     CDS             2567..3001
                     /locus_tag="VGT44_17355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582629.1"
                     /translation="MQIERIYKPDTANDSKASQTGTRKKPYEAFDSTGKPSPYTEIRC
                     VLQPSQSPQSRFFMAAVFSTDYDDAFTLLYSFVAVEVKGRNLKEMRRAIQIGRCEFIQ
                     EYNENEFLKPGKDAPVIESIQFITGEKLGNILATYKAGRQHA"
     gene            2994..3254
                     /locus_tag="VGT44_17360"
     CDS             2994..3254
                     /locus_tag="VGT44_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582630.1"
                     /translation="MLDGMFSFIDPIVELSEEDTRFVIQVEASFDFIEKIGLDKAASS
                     PEGTKVEVPLDIVMSLLRPDELEKLKNYITSYFTKRDGSEVK"
     gene            3406..>3552
                     /locus_tag="VGT44_17365"
     CDS             3406..>3552
                     /locus_tag="VGT44_17365"
                     /inference="COORDINATES: protein motif:HMM:NF036634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582631.1"
                     /translation="MDTSERSPANARIIKAPHGTTLTAKSWLTEAPMRMIMNNLDPDV
                     AEKPD"
BASE COUNT          822 a         1047 c          870 g          813 t
ORIGIN      
        1 cgctgcgctc ttggacgccg acctcagcgg ggccaatctc acgggcaccg acctgagcga
       61 ggccgtgctg aacagatcga gattgattgg agcgaacctg tccgcagcga ccctgcggga
      121 cgccgacctg caagatgcca cactcagggg cgccaacttg agcggggcca acctgcaggg
      181 tgccaacttg cgctacacgg tgctggccga tgccgatctc agcggcgcag acctcacagg
      241 gtgcgcgatc tacggcgttt ccgcgtggaa caccaggctg cacggcgcca tccaggcgcg
      301 ccttgacctc accgactctc ccgctccggg gcaccccgtc tcgcagcccg ccataacggt
      361 cggccatctg gcagccgcgc aattgctgca cctgctctct caaaatgcct acactccggc
      421 cgtccattcc gcgctcagcc gacacatcgt gttgatcctt gggcgcttcc cccccgaacg
      481 ccagcagacc ttgcaggcga tccgccacga cctgtgggag cgcaactatg tcccggtctt
      541 cgtggatgtg ctggccctgg aggagcaagc acacgccggc ctgatcgatg tgctgatcgg
      601 catggccagc ggcgtgctgg tcgatctttc cgatgcccca tacgcagagg tgatgttccc
      661 gcgtcgaccg atccccgtgc agccagtcct tgtggagggg caggtagcac gagaggtctt
      721 ccccctgttc taccaccaac tgctcccgct ctcccgctat gagagcacgg agggcttacg
      781 ccgctgcctg gcggtgtttc ttgaccgtct ctcgactgcc acacactgag cagcgccttc
      841 gccggtgaat gcacggctag ctccgggaag catgcggttc gcgctaaaaa gccacttgtc
      901 tctatcatgc aggcaattga atgcctttac atgatcccga ttgccgcgca tttttttata
      961 gaaattgggg cgcaaatgtc atgatagccg atttttcggg ggcgttcccg ccactccaca
     1021 ggcctcccta tacgattccg gctcccacaa cttccctaat tccattccgg tcttccctca
     1081 atgtcgtttc ttccctcaac agaagcaaag aatacctggt attgctggcg gatatatcaa
     1141 tgttccttga ccaaccgagg tgggcagtcg tctgctcgcc ctccactgat ttgcgacaaa
     1201 taacagtcag ttttgccgtt ttccctaact cgattcctgt tcttccctct cacttcctca
     1261 aagttggaac tgatcgcgct cggcgagggc ctcccatagg agaacctcca gacgggattg
     1321 cttgtagttc agcagctgca tcgttgccag agaacggcag tcttaatccc agcccaaccc
     1381 cggactgtcc gcacatgcgg acagtaaaag ctgcaatagc gaacgctagg cgtaatagcg
     1441 gcagctttcc acctggacgc cgtatacact tctgcaaagg ggtagatacg ctacagcctt
     1501 agcagagacg tgttttacct gtcgcgcatg ggttgccagt atctgaccgc acagatgcgg
     1561 aacaatctga atcggctttc cggcgatcgt cgtgaggatc agtccatcaa agagaaaatg
     1621 cagcaggtgc gtcacatcgc gctacgttaa ccggaccatt cgcccacttg cattctactg
     1681 atcatcagaa cgcaggcaac atttgggctg ttgcctacct ggctggatca aacctcggca
     1741 tatgacttcc ctcaatcacc agcgcttcct gctgcttttg cacgccgtct tcctgccccg
     1801 gatgaagcac caacgatcgg gcagctgccc atcttgattg cagaatatcc tctcgaagat
     1861 tcgccaggat ttaaacccac gcgggacgtg attagatcgg accagttcgg accgttcatt
     1921 ccctttcgat acctcttcta catttcatac atctttcgta tcatttcgac ttccactatt
     1981 acgtgcctgc ctcgcaaagg tagggactgg tacggacgac aggatgattt taaacacttc
     2041 ttctgctttt cacacatcta atgtctctaa taccagtgga agcggtccgg tcgagtccgt
     2101 acctgactgc ccatcttgtt tgggttggag gatcaaagag gcactgcttc cctcgattac
     2161 cctgatcgtc tgcttcataa caattcttcc tctccgagct tcctctggtc aacttttcgc
     2221 cttccctcag tcgcctatct tgcctgcctc ttctatctaa cttctccatc catctttttt
     2281 gccttccccc gattgcccga atcgcctgcc aggtgccacc tattccttac tcagctgcct
     2341 gatcacctac tgcaaaaaaa ttggataact ctcgtccttc gaacacttca tttagaaaca
     2401 acgagcttgt caaaggatag attggcagga taacggcagg ttgaaggcag gatagcgata
     2461 gatcgagaga attcgagcgg caatttaagg gcatccggtt gctgctagat tgctatcaag
     2521 cgatgtaaat ttatcgcgca caatctcagt agagcaggag acaaggatgc aaattgagag
     2581 gatttacaaa cctgacacgg ccaacgacag caaggccagc caaaccggca cgagaaagaa
     2641 accttacgaa gcatttgaca gcacaggcaa gccatccccc tatacagaaa tccgttgtgt
     2701 gctacagcca tcgcaatcgc cgcaatcgcg gttctttatg gcggcggtgt tcagcaccga
     2761 ttacgatgat gctttcacgc tgctctacag cttcgtggcc gtggaggtaa aagggcgcaa
     2821 cctgaaagaa atgcggcgag ccatacaaat tggccgctgc gaattcatcc aggagtacaa
     2881 tgaaaatgaa ttcctgaagc cgggtaagga cgcgccggtg attgaatcca tccaatttat
     2941 tacgggtgag aagctcggca atattctggc aacctataaa gcaggaagac aacatgcttg
     3001 acggtatgtt ttccttcatt gaccccatag tcgagctgag cgaagaggat actcgtttcg
     3061 ttattcaagt agaggcatcc tttgacttca tagaaaaaat cggccttgat aaagcggcaa
     3121 gttcgccgga aggaacaaaa gtagaggtgc ctttagatat tgtcatgtcc ttgctccggc
     3181 ccgatgagtt agaaaagcta aaaaactata ttacatccta ctttacgaaa cgtgatggta
     3241 gcgaagtgaa gtagccaggc acctggcgct gcctgagtga gccgcttctt agcgcctttt
     3301 ccagtgattg agagccgttt gacgaacact ctgcccattt gataccatct taagtaaacc
     3361 atcggaacca ttcattggca tgaccaaagc aaggaagaac cgcatatgga tacttctgaa
     3421 cgttcaccgg cgaacgcacg catcatcaaa gcgccacatg gcacgacgct caccgccaaa
     3481 agctggctca cggaagcgcc catgcgcatg atcatgaaca acctcgaccc ggacgtggca
     3541 gagaaaccgg ac
//
LOCUS       DASZAK010000992         3464 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_557700, whole genome shotgun sequence.
ACCESSION   DASZAK010000992 DASZAK010000000
VERSION     DASZAK010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557700"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="VGT44_17370"
     CDS             <1..995
                     /locus_tag="VGT44_17370"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2582632.1"
                     /translation="VNPRAVVRSTTSDHQLRRYIRMVPLILSQRVVGVLRLSMEDDPS
                     LLSVEQKLGVDRERTHGRAAFFWAFVDQAATVIERERLRRENLQVEVLQRTDALRAAL
                     LSSASHDLRTPLASIKAAASSLLEEDVQWDDEARRSFATSIVRETDRLNRLVANLLDM
                     SRIEGGALKPEKEWYPVDELVHDVLGRMQPLLQGRPIHTELPDDLPPVQLDYLQIDQV
                     LTNLLENAARYTPAGSPIEIDAHVENNDMVVQVMDRGPGIPAADRERVFDKFYRVMET
                     QTRATRTTGSGLGLAVCRGLIEAHGGHIWVDNREGGGAIFTFTLPLSSDEGTKR"
     gene            992..1690
                     /locus_tag="VGT44_17375"
     CDS             992..1690
                     /locus_tag="VGT44_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2582633.1"
                     /translation="MSKTGARILVVDDEIEIVRALYRSLDKHGFEVFTASSGEEALES
                     IALHRPDLMVLDLGLPGMSGLEVCRRVRAQSNLPIIVLSVKDTERDKVMALDLGADDY
                     VSKPFGMDEVLARIRVALRHTAQAQSGTEPIFTAGPLSVDFAQRQVHVNGQEVKLTPT
                     EYDLLKALIKNSGKIMTRQMLLSQVWGTGYGAESHYLHVYVGQLRRKIEPDPANPRFI
                     LTISGVGYRFNAEE"
     gene            complement(1697..2176)
                     /locus_tag="VGT44_17380"
     CDS             complement(1697..2176)
                     /locus_tag="VGT44_17380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582634.1"
                     /translation="MSLSTSTTTQSSTRTSSTRGLRWTTAITLILLAAQFLIGMLVNL
                     FVTVPAVHPGRDAPEYFSGVAQGIFWALLNSPLYLLLHVVVGLVLFLLSIVLLVMSIV
                     ARRAGWIVASILGFIGIMGAGFNGASFMNYNHDFSSLLMAIGFLLAAVSYVIALLRS"
     gene            complement(2192..2758)
                     /locus_tag="VGT44_17385"
     CDS             complement(2192..2758)
                     /locus_tag="VGT44_17385"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV2582635.1"
                     /translation="MYELIVLALLMRRPFHGYLIVKVTNDIIGPWAKISSGTLYPLLT
                     RMEKARLIVVVSVDESHGVAGERPSRTFSITEEGRKRFQQIMMDMSSNLGDYQRIFRF
                     KLVFFDLLQKQERLLLFNHYINYCQTSILYLQTEMANITYEITEPSEHRFLTNIVRTM
                     QHMVSQLQTELDWATSLRDQELAENDMP"
     gene            3029..>3464
                     /locus_tag="VGT44_17390"
     CDS             3029..>3464
                     /locus_tag="VGT44_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582636.1"
                     /translation="MNSLDGSEEIHQENDGANKSNKSEPRLIDEDLTGITRIEERRGG
                     QIFNERVRVVLPSRRSFRRISTGLLEATELSQAPQGGAAKFFYNVKRALIGAPLATAQ
                     QEHERLTKFKALAVLSSDAISSVAYATEAILITLVAAGSGVLG"
BASE COUNT          828 a          870 c         1020 g          746 t
ORIGIN      
        1 gtgtgaatcc gcgagcggtg gtgcgcagca cgacatcaga tcaccagcta cgacgatata
       61 tacgtatggt gccgctcata ctgagccagc gggtcgtcgg agtgctgcgc ctctcgatgg
      121 aagacgaccc aagtttattg agtgtcgagc aaaaactggg ggtagatcgc gaacgtacac
      181 atgggcgcgc ggcgtttttc tgggccttcg ttgaccaggc cgcgacggtc attgaacgcg
      241 agcgcctgcg ccgggaaaac ttgcaggtgg aggtcttgca gcgcaccgat gcgctccgtg
      301 cagcgctgct ctcgtcggcg tcgcatgact tgcgcacacc actggcatcg atcaaggcgg
      361 ctgcgagcag tttgctggaa gaagatgtgc agtgggatga cgaggcgcga cgcagtttcg
      421 cgacctccat cgtgcgggag acggatcgtt tgaaccggct ggtggcgaat ttgcttgata
      481 tgtcgcgcat tgaaggcggt gcgctcaagc cagagaagga atggtatccc gtcgatgaac
      541 tcgtacatga tgtcctgggg agaatgcagc ccctgttgca gggacgccca atccatacag
      601 agctgcccga tgacctgcca cccgtccagc tggactattt gcagatcgac caggtgctga
      661 ccaatctcct cgaaaacgca gcgcgctata ctcctgccgg ttcccccatt gaaattgacg
      721 ctcacgtgga gaataatgat atggtagttc aggtgatgga ccgggggccg ggcataccgg
      781 cggcggatcg cgagcgtgtc tttgataaat tttatcgcgt gatggagaca cagacacggg
      841 caacgcgcac gacgggttct ggcctcggtc tggcagtctg ccggggattg atagaggcgc
      901 acggcgggca tatctgggta gacaatcgtg aaggcggggg agcaatattt acgtttacat
      961 taccgctttc gagcgatgaa ggaacaaaac gatgagtaaa acaggagcgc gtatcctggt
     1021 ggttgacgat gaaattgaaa ttgtgcgcgc attgtatcgc agcctcgata agcatggctt
     1081 cgaggtcttc acggcaagca gcggcgagga ggctctggaa agtatcgcac tgcaccgacc
     1141 tgatctgatg gtgctcgacc tcggcttacc gggcatgagc ggcctggagg tctgccggag
     1201 ggtgcgggcg cagtcgaact tgccgatcat cgtgctgtcg gtgaaagata ccgagcggga
     1261 taaggtgatg gcgctcgacc tgggcgctga cgactatgtt tccaagccgt tcggcatgga
     1321 tgaagtgctg gcgcgtatcc gggtggcgtt gcgccatacc gcccaggcgc aatcaggcac
     1381 agagccgatc ttcactgccg ggccgctcag cgtggatttt gcccagcgcc aggtgcatgt
     1441 caacgggcaa gaggtcaagc tgacgccaac cgaatacgat ctcttgaagg cgctcatcaa
     1501 gaatagcggc aaaattatga cgcgccagat gctgctctcc caggtctggg gaacgggtta
     1561 cggcgcagag tcgcattacc tgcacgtcta tgtcgggcag ctgcgccgca agatcgaacc
     1621 agacccggcc aacccacgct tcattctcac catttcaggt gtcggttatc gattcaacgc
     1681 agaagagtag ttctctctat gaccggagta gggcgatcac ataggaaacg gcagccagga
     1741 gaaaaccgat cgccatcagc agcgagctga agtcatggtt atagttcatg aagctcgcgc
     1801 cattaaatcc agctcccatt atcccgataa agccgaggat cgaagcgacg atccaaccgg
     1861 cacgacgggc gacaatcgac atgacaagca gaacgatcga gagcagaaat aagactagcc
     1921 cgacgacaac gtgcagtaac aagtacaatg gactattgag caaggcccag aaaatgccct
     1981 gtgcgacccc agagaagtat tcgggagcgt ctctacctgg atgaacagca ggaacagtga
     2041 cgaagaggtt aaccagcatc ccgatcagga actgggccgc cagcaggatc agagtgatgg
     2101 cggttgtcca acgcaatcct cgtgtgctgg acgttctggt tgatgattgg gtagtagtgg
     2161 atgtcgaaag tgacattgtg caatcctttc ttcagggcat gtcgttttcc gcaagttcct
     2221 ggtcccgcag gcttgtggcc caatcgagtt ccgtctgcaa ctgactcacc atgtgctgca
     2281 ttgttcgcac aatattggtt aggaagcggt gttctgaagg ctcggtaatt tcatacgtga
     2341 tatttgccat ctccgtctgc aaatacagga tggaagtctg acaatagttg atgtagtggt
     2401 tgaagagcag gagacgttcc tgcttctgca ataaatcgaa gaataccagt ttgaaacgga
     2461 agatgcgctg gtagtctccc aggttggaag acatgtccat catgatctgc tgaaaacgct
     2521 tgcgtccctc ctcagttatg gaaaacgtgc gcgatggccg ctctcctgcc acgccatggc
     2581 tttcatcgac agagacgact acgatcaacc gggccttttc catacgggtt aataacgggt
     2641 agagtgtgcc gctgctaatt ttcgcccagg ggccgatgat atcgttggta actttcacga
     2701 tcaggtaccc atgaaacgga cgccgcatca gcagggcaag tacaatcaat tcatacatgc
     2761 cagggaacac tttctttcac tgtgataata cctttaataa tacctttaat agaggtattg
     2821 tacctgtgtt attcgaggat gtcaagcgtc atccggtcag aagagactaa aagtttgcgg
     2881 taggaactca agattcgctc aataaacagg gtaaaatgct caagatcatg tggcttttgc
     2941 tcgctcattc ttgattagtt gagagaatgg tcgctatact ggtaacagaa ataagctctc
     3001 cctttactaa ttacaggaag tgatagacat gaacagcctg gatggctctg aggaaataca
     3061 tcaagagaat gacggcgcga ataaatcgaa caagagcgag ccgcggttga tcgacgagga
     3121 cctgaccggc atcactcgta tagaggaacg ccgcggtggg cagatattca atgagcgtgt
     3181 gcgcgtggta ctgccaagcc gccgctcctt ccggcgcatc agcaccggcc tgctggaagc
     3241 gaccgagcta tcccaggcgc cgcagggagg cgcggcgaaa ttcttttata atgtgaagcg
     3301 agcgcttatc ggtgcgcctc tcgcgacggc gcagcaagaa catgaacgct tgacgaagtt
     3361 caaagcgctg gcggttctct cttccgatgc gatttcctcc gttgcctatg ccacggaagc
     3421 gattttgatt acgctggtcg cggcaggatc gggtgtgttg gggt
//
LOCUS       DASZAK010000993         9827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_344746, whole genome shotgun sequence.
ACCESSION   DASZAK010000993 DASZAK010000000
VERSION     DASZAK010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9827
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344746"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..971)
                     /locus_tag="VGT44_17395"
     CDS             complement(<1..971)
                     /locus_tag="VGT44_17395"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HEV2582637.1"
                     /translation="MNGRQFDVVAAGEILVDLISDDAQSDLVGSSSFTMYPGGSVANV
                     ALNVASLGGAAALFARVGSDAFGTFLQQRVQDARVDTSLLSSSSHSPTTAVVVTRQQQ
                     RPDFIAYRGADIELESAAFPAALLADARWLHTSAFALSYEPARSTLLRVIAQARSAGC
                     YISFDPNYHPRIWERDVDPFPFISSVCSYSTLVKPSLDDCTRLFGPGASPEAYAAHFR
                     AWGAEQVLLTMGQKGVLLVNAAGHMYFPAQKVNVVDVTGAGDAFLAGVLLARRDGLSY
                     PDAVRFAQAVAAMKLQQLGPLHHQIDRFEMYDSLGLQGYEIASPENL"
     gene            complement(961..1998)
                     /locus_tag="VGT44_17400"
     CDS             complement(961..1998)
                     /locus_tag="VGT44_17400"
                     /inference="COORDINATES: protein motif:HMM:NF012475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582638.1"
                     /translation="MVFIPEQSYLWDFWLVSPQEWGDARAPFHMYYLQAPRTLSDPNA
                     RHNVATVGHAVSHDLRHWEQCGTALEAGRPGSWDDQATWTGSVIVRDGLAYMYYTGKS
                     YAERGLVQRIGLAVSNDLIHWERHLDNPILEVNTRWYEPQSAERRETQTWRDPYIVYS
                     SVEEAYYMFLSARVNTGLLDGRGVIGLARSTDLLSWEVLPPVNVPGDFTEMEVPQLVP
                     LNGRYYLLFCAARHAAARLPPAQGGGWYGTHYLVASKLTGPYYPLTDEPLVADALGTY
                     YAGKLVYNNAGGLSFMAWRQWDEGGNFCGGLSNPAAVRVLPDGRLQVDRQQLWTADVV
                     HRVSKEPIDER"
     gene            complement(2014..2853)
                     /locus_tag="VGT44_17405"
     CDS             complement(2014..2853)
                     /locus_tag="VGT44_17405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2582639.1"
                     /translation="MNRQRRVSLVIKYVVLSVIALMFLFPFAWMILTSLKSNGEALTN
                     PTSLLPAVLQWGNYPDVINALNIWRETFNSLVMALGVTLGQIILAALAGYAFARLKFF
                     GRDVLFLLILATLIIPFQILFVPLYLMLAGWGGWLNSFASLIVPSLASPFAIFVFRQF
                     FITVPKELDEAARIDGAGYYRTFFSVMLPLAGPAVATVFILTFLSEWSNFLKPLVFTS
                     SPDMYTLQVGLAVTLSRGANLVPNVATLMAGVVLASIVPVVMFLVGQKYFVRSVASTG
                     IKG"
     gene            complement(2876..3820)
                     /locus_tag="VGT44_17410"
     CDS             complement(2876..3820)
                     /locus_tag="VGT44_17410"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2582640.1"
                     /translation="MQIQQVQEQAKIRVVSPRQSNLKGIIRQVQAFSFTLPALVLLAI
                     FLIYPIGYVVYLSFQRWDLLGSPTFIGLQNYNTILFRDSSFLQSVGVTVFFVVLAVPA
                     QVGLGLFLAILLDSQFKGRTFFRTVFFIPMVISYVAAGIVFQWLFSTGANLGIFPQLF
                     ANIGITFPDWQHTNGIWAMIIVVVMNTWKATGFSMILYLAGLQSINPDYYEAGQIDGI
                     RNAWQRFRYISWPLLVPTTTLLIITNTIGSFQAFDPFYVMTNGGPSGATNTLVFYIYT
                     NFTSRTGVATAAATVFLIGVLMITAVQLLVARRRETYY"
     gene            complement(3914..5236)
                     /locus_tag="VGT44_17415"
     CDS             complement(3914..5236)
                     /locus_tag="VGT44_17415"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2582641.1"
                     /translation="MATPFRYILNRREFLKRATAVGLSASSAAALLEACGSSTTSGNG
                     KVNVNLYHTIPAATETFWKQQLLPQFEKQYPGMNLITYQLGVENPAVIATKIKAGGDT
                     APDMAWLESGLTGTYVQANLLADVDGWFNSHPTVKQDVFPSLITLSSYQGKVWSFPWM
                     TNNTAMQINLDAFEKAGVPIPSQDPTKTWTWDQFAEACKLVTQKAGMKGFLMNNGGAG
                     WDAWLFHAWLGTNGGTFIDANGNPLFSGNEGAATMTFLQSLVKDGYTAFSAPGMGYDP
                     SGWYSGKAAIMANGPWNFPDLVKFTSFKFTVVPYPVNKQPATNTGGDQLYVFNNDPQK
                     VAYSFDYAQYMLTDDFQTAFNIESGNLPVTQSATSSAAYQAHLKQYPFLNGWVNGVPY
                     GVARSSLPQSSDAQTAFGQKAWDPIILQGADVQQALANAASAVTALKP"
     gene            5492..6502
                     /locus_tag="VGT44_17420"
     CDS             5492..6502
                     /locus_tag="VGT44_17420"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2582642.1"
                     /translation="MTATIHDVALEAGVGIGTVSRVVNNSPSVRPTTRDRVQAAIDKL
                     HYKPDPIARSMISKRTNSLGVIVPFFTRPFFMEVLRGVESVSDRMERELVLYNVETNA
                     QRDRLFSELPMHRKVDGLLIMSLTPDDAAACRLRDSGLPIVLIDAYSPMLTSLVVNNV
                     EGAYQAVRCLIENGHQRIGFINGEIEGNFKFNQANDRLIGFHRAFGEAGLLFEPELVL
                     TSEWNRAGGKQAALQLLSQQKRPTAIFAASDMQAVGVLEAARALGIAVPEQLSVMGFD
                     GIEISELLELSTVQQPMREMGEVGATALLKLIDNPSTAPELMRFDTRLVERHTTGPVP
                     AQ"
     gene            6592..7902
                     /gene="melA"
                     /locus_tag="VGT44_17425"
     CDS             6592..7902
                     /gene="melA"
                     /locus_tag="VGT44_17425"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV2582643.1"
                     /translation="MTTPPYITLIGAGSAVFARQLITDILQIEGLDEGCFALVDIDPV
                     RLQLAYQVAERLVALSGKRWIVRASTERRDLLAGSDFIINSIEVAGLANVRHDFDIPM
                     KYGINQCIGDTIGPGGIFKALRTGPAWLDILHDAEQLCPQAWVLNYTNPMSILTLAAL
                     LGSKVRTVGLCHSVQGTSKQLADYLEVPYVELEWRCAGINHNAWFTELRCQGQDMYPR
                     LRERSEVSEVYERDPVRFEMMRYFGAFVTESSGHFSEYVPYFRKRPALIERYCRAGYL
                     GETGFYANNWPAWRAQNDRSIQEMLDGTQEMPYSRSHEYGSVIVEAMTLSRPAVIYGN
                     VRNSGLIDNLPDGCVEVACLVDRNGVQPCHFGALPEQLAALNRSHMAVHSLMCEALLT
                     RNKEAARYALLLDPLSAAVCSPAELSALFDEMWEAEREYLQPFE"
     gene            7912..8577
                     /locus_tag="VGT44_17430"
     CDS             7912..8577
                     /locus_tag="VGT44_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HEV2582644.1"
                     /translation="MMTRVTIWNEFRHERTDPHVTALYPQGIHATLAEGLSHAEFAIR
                     TATLDEAEQGLPDAVLSTTDVLLWWGHLAHEEVADALVERVHQRVLDGMGLIVLHSGH
                     YSKIFRRLMGTSCSLQWREADDRERLWVTAPGHPIVEGIGECIDLEREEMYGEYFDIP
                     QPETLVFVSWFTGGEVFRSGCCYSRGRGRIFYFRPGHEAFPTYAHPQIQRVIANAVRW
                     AAQ"
     gene            8749..9780
                     /locus_tag="VGT44_17435"
     CDS             8749..9780
                     /locus_tag="VGT44_17435"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2582645.1"
                     /translation="MTIRLIQVGMGGWGRNWATHVLQPSEDVELVACVDPDAGMLAQA
                     QQLLNLPPERYFPTLEAALSAVEAEAVLITASLPAHVPIALEALCAEKHVLIEKPFAP
                     TLAEARQVVETAAQQRRMLMVSQNYRFFPAVQAVRALVRERNLGEIGAVRIDFRYYKN
                     SDQPADQRYAQVQQPLLMDMAIHHFDLMRVVLGQEPTRITCQSWNPPWSKYVDSASAA
                     ATITFDGGTVLSYRGSWVSPGTRTSWSGEWHMECSGGEIVWASRDATAPDYVTLRPLG
                     KRAQHLKLPTLAYTDRSGSLHAFVQAIQAGQEPESSGRDNLPSLALMFAAVESATSHV
                     PVSLHGMKY"
BASE COUNT         2205 a         2825 c         2844 g         1953 t
ORIGIN      
        1 atcaaattct ccggcgatgc gatctcgtat ccttgcaatc ccaggctatc gtacatctcg
       61 aaacgatcga tctggtgatg cagcgggccg agctgttgca gtttcatcgc cgcaaccgcc
      121 tgcgcaaatc gaaccgcgtc agggtacgac aaaccatcgc gccgcgccag cagaacgccc
      181 gccaggaatg catcccctgc accggtcaca tcaaccacgt tcactttctg agcagggaaa
      241 tacatatgtc ccgccgcgtt gacgagtaag acaccctttt gtcccatcgt caatagaacc
      301 tgctcggctc cccatgcccg gaaatgagcg gcataggctt cgggcgaggc gcccggtcca
      361 aaaagacggg tacagtcgtc gagcgaaggt ttgaccagcg tcgaatacga acatacgctg
      421 gaaataaatg gaaaaggatc aacatcacgc tcccagatgc gcggatggta attcgggtca
      481 aagctgatgt agcacccggc tgagcgcgcc tgggcaatca cgcgcaggag cgtagagcgg
      541 gcaggttcgt aggagagcgc gaaagccgag gtatgcagcc agcgggcatc cgccaggagc
      601 gcggcgggga aagcagcgga ctccaactcg atatccgcgc cccggtaagc gataaagtca
      661 gggcgctgct gctggcgggt gacaacgact gcggtggtgg gactgtgcga tgaggaggaa
      721 agcaagcttg tatcgactcg cgcgtcttgt acgcgctgct ggagaaaggt cccaaaagca
      781 tcggagccaa ccctggcgaa gagcgccgcc gctccgccca ggctggctac gttaagcgcg
      841 acattggcaa ccgaaccacc gggatacatg gtgaaactgc tcgatccgac caggtcgctc
      901 tgggcgtcat ctgaaatcag gtctaccagt atttcgccgg cagccactac gtcaaattgt
      961 ctaccgttca tcaatcggct ctttgcttac acgatgcaca acatcagcag tccacaactg
     1021 ctgtcgatcg acttgcaaac ggccatcggg caggacacgc actgcggcag ggtttgagag
     1081 gccgccgcag aagttgcccc cttcatccca ctgccgccac gccatgaatg agagtcctcc
     1141 cgcgttattg taaaccagtt tgccagcata ataggtacca agggcgtcag cgacgagcgg
     1201 ctcgtcggtg agcgggtaat agggtccggt aagcttgctg gcaaccaggt agtgcgtacc
     1261 gtaccagccc ccaccctgtg cgggaggcag gcgcgcggca gcgtgcctgg ccgcgcaaaa
     1321 cagcaggtag taccggccat tcagcgggac gagctgcggc acctccatct cggtgaagtc
     1381 acccggcacg ttcaccgggg gcaagacctc ccacgaaagc aagtcggttg agcgcgccag
     1441 gccaattacg ccccggccat caaggaggcc ggtattgact cgcgctgaca ggaacatgta
     1501 gtacgcttcc tctacggatg agtatacaat ataaggatcg cgccaggtct gtgtctccct
     1561 tcgctcagca ctctgcggct cgtaccagcg cgtattaacc tcgaggatgg gattatccag
     1621 gtgtcgttcc caatggatga gatcgttcga gacggccagc ccgatgcgct gcaccagtcc
     1681 cctctcagca tagctcttcc ccgtgtagta catgtatgca aggccatcgc gcacaatgac
     1741 gctccccgtc caggtggcct gatcgtccca actacctgga cgaccggcct ccagcgcggt
     1801 tccacactgc tcccagtgcc gcagatcgtg cgaaacggca tgtccaaccg tcgctacgtt
     1861 gtggcgtgcg ttcggatcgc tcaacgtgcg cggcgcttgc aggtaataca tgtggaaggg
     1921 cgcgcgcgcg tccccccact cttgcgggga gacaagccag aaatcccaca gataggactg
     1981 ttccgggata aaaaccacgt taaacggccc tctttatccc tttatgccgg ttgaggcaac
     2041 gctgcgcacg aagtatttct gaccaaccag gaacatgacg acaggcacga tgctggccag
     2101 cacgacgcca gccatcaacg tcgccacgtt cgggacgagg ttcgctcctc tgctgagcgt
     2161 gaccgccaat cccacttgta gcgtatacat atccggcgac gaggtgaaga ccagcggctt
     2221 gagaaagttg ctccattcac tcaggaaggt caggataaaa acggtcgcta ccgccggacc
     2281 tgcgagcggc agcatcaccg aaaagaaggt acggtagtaa ccagccccgt cgatccttgc
     2341 cgcctcgtct agctccttcg gcaccgtgat gaagaactgg cggaagacga agatggcaaa
     2401 cggactggcc aggctgggaa caatcagaga ggcgaacgag ttcagccacc caccccagcc
     2461 ggcgagcatg aggtagagcg gaacgaacag tatctggaag gggatgatga gcgtcgccag
     2521 gatcagcagg aacagcacat cgcgcccgaa gaatttcaaa cgcgcaaagg catacccggc
     2581 cagcgctgcc aggataatct gacccagggt gacacccagc gccatgacga gcgagttgaa
     2641 ggtttcgcgc cagatattca gggcgttgat aacatcgggg tagttgcccc actgcaggac
     2701 ggctggcaac aacgatgtcg gattggtaag cgcctcacca ttggatttga gcgaggttaa
     2761 aatcatccag gcgaatggga acaggaacat tagggcgatc acgctcagga caacgtattt
     2821 aatgacaagg ctcacgcgcc gctgcctatt cataaccgat ctccttccag actgattagt
     2881 agtacgtttc gcgccggcgc gcaactagca gctgcaccgc cgtaatcatc aggacgccga
     2941 tcaggaaaac cgtcgccgcc gccgtcgcga cacccgtgcg gctcgtgaaa ttagtgtaga
     3001 tgtagaacac tagcgtgttg gtcgccccgc tcggtccccc gttggtcatg acatagaaag
     3061 gatcgaacgc ctggaagctg ccaatcgtgt tggtgatgat cagcagtgtg gtcgtcggta
     3121 ccagcagcgg ccacgaaatg tagcggaagc gctgccaggc gttgcggata ccgtcgatct
     3181 gcccggcctc gtagtaatcg ggattgatcg actggaggcc ggccaggtag agaatcatcg
     3241 agaaccccgt cgccttccac gtgttcatca ccacgacgat aatcatggcc cagatgccgt
     3301 tggtatgctg ccaatcgggg aacgtgatgc caatgttggc gaacaactgg gggaaaatgc
     3361 ccaggttggc cccggtcgag aacaaccact ggaagacgat cccggcggcg acataagaga
     3421 taaccatcgg gataaagaac acggtgcgga aaaaggtgcg tcctttgaac tggctgtcga
     3481 gcaggatggc caggaacagc cccagcccga cctgcgccgg gactgccagc acaacgaaga
     3541 acacggtaac gccgacggac tgtaaaaagc tcgaatcccg gaagagtatg gtgttgtagt
     3601 tctgcaggcc gataaacgtg ggacttccga gcaaatccca gcgctgaaag ctgaggtaga
     3661 cgacataacc gatgggatag atgaggaaga tagcaagcag cacaagggcg ggcagcgtaa
     3721 aggagaacgc ctgcacctgc ctgataatcc ccttcaagtt actttgtcgc gggcttacga
     3781 ccctgatttt ggcctgctcc tgaacctgtt gtatctgcat cgatacctcc tggatgttat
     3841 ccgaacaaca ataggacgcg ggcagcgcag cgcagcatgc gctgcccaat agcccaacaa
     3901 ctgataggtc ctgctatggc ttgagcgctg tgaccgccga cgctgcattc gcaagagctt
     3961 gttgcacgtc ggctccttgc aaaatgatgg ggtcccatgc cttctgacca aaagccgtct
     4021 gagcatcact tgattgcggc aggctggagc gcgcgacgcc atacggaacg ccgttgaccc
     4081 acccattcag gaaggggtat tgcttgagat gagcctggta agccgcgctg cttgtggcgc
     4141 tctgcgtcac cggcaggttc ccagactcga tattgaatgc cgtctggaag tcatcggtga
     4201 gcatgtactg cgcatagtcg aatgagtaag ccaccttctg cgggtcattg ttgaagacgt
     4261 acaattgatc gccgccggtg ttggtggccg gctgcttgtt gaccgggtag ggaacaaccg
     4321 tgaacttgaa cgacgtgaat ttgacgaggt cagggaagtt ccaggggcca ttggccataa
     4381 tcgccgcttt gccgctgtac cacccactcg gatcatagcc catacctggg gcagagaacg
     4441 cggtgtagcc gtccttaacc aggctctgca ggaaggtcat ggtcgccgct ccctcgttgc
     4501 cactgaagag cggattgccg ttggcgtcga tgaatgtgcc gccattggtt cccaaccagg
     4561 catggaagag ccaggcatcc caccctgcgc cgccattgtt catcaagaag cccttcatgc
     4621 ccgctttctg cgtgaccagc ttgcacgctt cggcgaattg atcccatgtc caggtcttcg
     4681 tcgggtcctg cgatggaatg gggactcctg ccttctcgaa tgcatcgagg ttgatctgca
     4741 tggccgtgtt gttcgtcatc caggggaaac tccacacttt cccttgatac gacgagagtg
     4801 tgatgagcga ggggaagaca tcttgcttga cggtaggatg cgagttgaac cagccatcca
     4861 catccgccag cagattcgcc tggacatatg ttccggtcaa tccactttca agccatgcca
     4921 tatctggcgc ggtatcgcca ccagctttga tcttggtggc gattactgct ggattttcta
     4981 caccgagctg ataagtgatc agattcatcc ccggatactg cttctcgaat tgtggaagca
     5041 gttgctgctt ccagaacgtt tcggtcgccg ccgggatggt gtgatacagg ttgacgttca
     5101 ccttgccatt tccagaagtc gttgacgaac cgcaggcttc gaggagtgcc gctgcgctcg
     5161 acgcgctcaa tccgacggca gttgcccgtt tcaaaaactc acggcggttg aggatgtaac
     5221 gaaatggagt cgccataaaa actctccttc gattgaagac agtgctgcat agcaccgcta
     5281 gggcagaggg tctacaaata tacgtgccga aagttgattt gtggaagcgt ttccataatc
     5341 tgctagcaac tataccataa gatttctgaa ttgtaaagag gttcaggcac tttacagaag
     5401 accttgtcta tgttatgcta aatttaggta gcatgtttga agatgaaagc gttttcaccg
     5461 gaatatctac ctagagaaag atcacttagc aatgacagcc accattcacg atgttgcgct
     5521 agaagctggc gttggcattg gaaccgtttc acgggttgtc aacaacagcc ccagcgtcag
     5581 gcccacgacc cgcgatcgag tgcaggcggc aattgataaa ctccactaca agcccgaccc
     5641 tatcgcgcgc agcatgattt caaagcgcac gaactctctg ggcgtcatcg tgcctttctt
     5701 cacccgcccc ttcttcatgg aggtcttgcg cggtgtcgaa agcgtgagcg accgcatgga
     5761 gcgtgagcta gtgctctata acgttgagac gaatgcccag cgcgaccgct tattttctga
     5821 gcttcctatg catcgtaaag tggatggttt gctaattatg tcactcacac ccgacgacgc
     5881 ggcggcgtgt cgattacggg attcgggact accgattgtg ttaatcgatg cttatagccc
     5941 catgttaacc tcgctggtag tgaataatgt tgaaggcgcg taccaggccg tgcggtgtct
     6001 catcgaaaac ggacatcagc ggattggctt catcaacggg gagattgaag ggaacttcaa
     6061 atttaaccag gccaacgacc ggctgatcgg cttccatcgg gctttcgggg aggcaggact
     6121 gctcttcgag ccagagctgg tacttacctc ggagtggaat cgggcggggg gaaagcaggc
     6181 cgcgctgcaa ctgctctccc agcagaagcg cccgacggct attttcgcag cttcggatat
     6241 gcaggccgtt ggcgtgctgg aagcagccag agctttgggt attgccgtac ccgagcagct
     6301 ttccgtgatg ggatttgatg gcattgagat ttcagaactg ttagaactca gcacagtgca
     6361 acaaccaatg cgcgagatgg gcgaggtagg agctacagcg ctgttaaaac tcatcgacaa
     6421 cccgtcaacc gcgccagagc tgatgcgttt cgatacccgc ctggtagagc gccacacgac
     6481 cggcccggta cccgctcaat aagcgaggca ggcttaattt ctttcactcg gaagcgtttc
     6541 caataacgtc accctgaccg gctctagtcg aaagccatag gaggataagc catgactaca
     6601 ccaccgtaca tcacgctcat cggcgcgggc agcgccgttt tcgcccgcca gctaatcacc
     6661 gacattttgc agatcgaggg actcgacgag ggctgtttcg cgctcgtgga tatcgaccct
     6721 gtgcgcttgc aactggccta ccaggtcgcc gaacgactgg tcgcccttag cgggaagcgc
     6781 tggatcgtgc gcgcctccac cgagcgacgc gacctgctgg ccgggagcga tttcatcatc
     6841 aactccatcg aggttgccgg gcttgccaac gtgcgccacg atttcgacat tcccatgaag
     6901 tacggcatca atcaatgtat cggtgacaca atcggcccgg gcggtatctt caaagcgctg
     6961 cgcaccggtc ccgcctggct cgatatcctg catgacgccg agcagctctg cccgcaagcc
     7021 tgggtcctca actacaccaa cccgatgtcg atcctcaccc tggccgcgtt gctcggcagc
     7081 aaggtgcgca cggttggctt atgccatagc gtacagggta cctcgaaaca actggccgac
     7141 tacctggaag ttccctatgt ggagttggag tggcgctgcg cgggcatcaa ccacaatgcc
     7201 tggttcaccg agttgcgctg tcagggacag gatatgtacc cacgcctgcg cgaacggagc
     7261 gaggtatcgg aagtgtacga acgcgacccc gtccgcttcg agatgatgcg ctacttcggc
     7321 gcgtttgtga ccgagagcag cggccacttc tcggaatacg tcccctactt ccgcaagcgg
     7381 cccgctttga tcgagcgtta ctgccgggcg ggctacctgg gtgagaccgg cttctacgcc
     7441 aacaactggc cagcctggcg cgcccagaac gaccgctcaa tccaggagat gctggatggg
     7501 acgcaggaaa tgccctattc acgcagccac gagtacggtt ccgtcatcgt cgaagccatg
     7561 acgttgagcc gacccgccgt catctatggc aatgtgcgca acagcggcct gatcgataat
     7621 ctgcccgatg gctgcgtgga ggtagcctgc ctcgttgacc gcaacggcgt gcagccctgc
     7681 cattttgggg cgttaccaga gcagcttgcc gcgctcaatc gcagccacat ggccgttcac
     7741 tcgctcatgt gcgaagccct tttgacgcgc aataaagagg cggcgcgcta tgccctgctg
     7801 ctcgaccccc tcagcgcggc ggtgtgttcg ccagccgagc tgagcgcgct attcgacgaa
     7861 atgtgggagg ccgagcgcga gtacctccag ccctttgagt aattaggagg aatgatgacg
     7921 cgcgtcacta tctggaatga gtttcgtcac gagcgcacag acccacatgt cacggcgctc
     7981 tatccacagg gcatacatgc cacgctcgcc gaggggctga gccacgcaga gtttgccata
     8041 cgcactgcta cccttgatga agctgaacag ggactccccg atgccgtgct tagcacgacg
     8101 gatgtgctgc tctggtgggg ccatctcgcc catgaagagg tggcagatgc gctggtggaa
     8161 cgcgtgcatc aacgtgtttt ggacggcatg ggattgatcg tactccattc gggccattac
     8221 tcaaaaatct ttcgacgcct gatggggacg agttgcagtc tgcaatggcg cgaggcggat
     8281 gaccgtgaac gcttatgggt aacagcccca ggacatccga tagtggaagg gatcggcgag
     8341 tgtattgacc ttgagcgcga agagatgtac ggcgaatact tcgatattcc tcaaccggaa
     8401 acactcgtgt ttgtgagctg gtttaccggc ggcgaagtct ttcgcagcgg ctgctgctat
     8461 tcacgcggac ggggcagaat cttctacttc cgtccgggac atgaggcgtt tccaacttat
     8521 gctcacccac aaatacagcg cgtgatcgcc aacgccgtac gctgggcggc gcagtgacca
     8581 caaatgggtc ggtaggtacg cagccaacac aagatgtcat cctgagcgca gcgaaggatc
     8641 ttgttccccg gcgcgtcaga cccttcgctg cgctcagtgt gacatccgag gtgtggactg
     8701 cgcccatacc gaccatcgta tttgaaatag aaggagaaag aaagcgccat gacgattcgt
     8761 cttatacagg taggtatggg tggctgggga cggaactggg ctacccatgt actccagccg
     8821 agcgaggacg ttgaactggt cgcctgtgta gacccggacg cggggatgct tgcgcaagcg
     8881 cagcagctcc tcaacctccc accagagcga tatttcccaa ccctcgaggc ggccctgtca
     8941 gcagttgagg ctgaggcggt actcatcacc gccagcctgc cagcgcatgt cccgattgct
     9001 cttgaagcgc tctgcgcgga gaaacacgta ctgatcgaaa agccatttgc tccaaccctg
     9061 gcggaggcgc ggcaggtggt cgaaacggcg gcacaacagc gccggatgct gatggtcagc
     9121 cagaattacc gcttctttcc agccgttcaa gccgttcgcg cattggttcg cgaacggaac
     9181 ctcggcgaga ttggcgcggt gcgtatcgac ttccgctatt acaaaaatag cgatcaaccg
     9241 gcagaccagc gctacgccca ggtacagcag ccgctgctga tggacatggc catccaccat
     9301 ttcgacctga tgcgcgtggt gcttggtcag gagccaaccc ggatcacttg ccagtcatgg
     9361 aacccaccct ggagcaaata cgtcgattct gcaagcgcgg cggcgacgat caccttcgat
     9421 ggtggtacag tgctaagtta ccgtgggagc tgggtcagcc ccggcacgag gaccagctgg
     9481 agtggagaat ggcacatgga atgcagcgga ggggagattg tctgggcaag ccgggatgct
     9541 acagctcctg attacgttac acttcgtcca ttagggaaac gcgcgcaaca cttgaagctg
     9601 cctacgctgg cgtacacaga ccggagcggg agcctgcacg cctttgtgca ggccattcag
     9661 gcgggacaag aaccagaaag ctctggacgt gataatcttc cctctcttgc gctgatgttc
     9721 gcagccgtcg agtcagcgac ctcgcacgta ccggtctccc tgcatgggat gaaatattaa
     9781 atcgggcatc tccgtaggtg ccggttcacc gcgcacaccg ccgattg
//
LOCUS       DASZAK010000994         4635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_381931, whole genome shotgun sequence.
ACCESSION   DASZAK010000994 DASZAK010000000
VERSION     DASZAK010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381931"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VGT44_17440"
     CDS             <1..420
                     /locus_tag="VGT44_17440"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582646.1"
                     /translation="NAVGGHRLANFRGHEGWVRALAWSPDGKRIASTGEDKAVQVWDA
                     ARGRLIHTHRGHADWVNSLSWSLDGRRVASISKDAVIQVWDAEPSLHSSTPRSQTSGG
                     PLAFHANPSSIHAVTWLPDDKHIAVACGDGSVQVWQA"
     gene            496..1275
                     /locus_tag="VGT44_17445"
     CDS             496..1275
                     /locus_tag="VGT44_17445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV2582647.1"
                     /translation="MLVDSHAHIDTARFNADREAVIQAAFEGGVTRIVDPGCDLPSSE
                     ATLALAKAHPGMIFAGVGTHPHDATTYSDEVGQRYREMAREPEVVAIGECGLDYFRML
                     SPRDVQRAVFVAHLELARACDLPCIIHVRDSHDDVIELLRAHGQGLRGVFHCFSGDVA
                     QAEQCLEFEGFLLSFAGPLTRPGNALPEVARIAPLERILVETDSPYLVPQPLRAKRNE
                     PLFVRYVAEKLAELRDMTFAEIAQVTTANAVRLFGLGEIAQ"
     gene            1272..2459
                     /gene="cysS"
                     /locus_tag="VGT44_17450"
     CDS             1272..2459
                     /gene="cysS"
                     /locus_tag="VGT44_17450"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV2582648.1"
                     /translation="MKLFNTLTQSLEEFVPLKEKTVRIYVCGVTPYDTTHLGHAFTYV
                     SFDTLIRYLEFRGYTVNYVQNVTDIDDDILRKAREVGLAWDELGRRETERYLNDMDAL
                     NVRRPNVYARATQETPTMIEIVRKLIADGYAYQSGGNVYFSVKRDPDFGVMARAIGLN
                     DYESMLAIANERGNFPDDTRKRDPLDFVLWQAQAPGEPSWPSPWGPGRPGWHIECSAM
                     SMKYLGPQIDIHGGGTDLAFPHHTCEIAQSEHFTGKAPFSRFWMHTGMVHQDGEKMSK
                     SLGNLTLVSDLLKIYSADTCRVTLLNHHYRYPWECFPDDLQVASETVALFQQIRSIVG
                     DHTGGTDHLLHDRFIAAMDNDLNTPQALLLLRQCAETVVANRDINAGAEILRLTKVLG
                     LRL"
     gene            complement(2448..3422)
                     /locus_tag="VGT44_17455"
     CDS             complement(2448..3422)
                     /locus_tag="VGT44_17455"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HEV2582649.1"
                     /translation="MNRTDRLLAIVLELQGKGHQRAEDLAATFETSKRTIYRDMLALG
                     EAGVPIVSMPGRGYSLMQGYFLPPLSFTTDEATMLLLGSDFMAQNFDAQYREAAQSAS
                     RKIEGALSEKLRDEVRYLQSSIRFVAASPQTPENARRIALLQQLRRAIIQHTTVRFTY
                     HARHSQDGKSEQTRREADPYGLIHFPAGWHMTGYCHLRQGIRNFRLDRIDDLELLPAT
                     FQRPANFVMRRDRESDREIVVRVLFDHDIARWVQEARSYYVVAEEETPDGLLVTLTIR
                     QESEILHWLLSWGRHARVLEPESLRERLAEEAEGMVTQYKGRVARLQT"
     gene            complement(3432..>4635)
                     /locus_tag="VGT44_17460"
     CDS             complement(3432..>4635)
                     /locus_tag="VGT44_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEV2582650.1"
                     /translation="GLVIFTTGSAICGASFSLGMLIAARALQGLGGALIMAVSPAILT
                     SAFPASERGRALGMNAVFVALGTSVGPTLGGIITANFTWRWIFYVNVPLGIIGIIATL
                     RILKEPRRSGQERGRFDPLGAILLAIGLVCLTLGLSFGQEWGWGSPLLIGTIVVSACA
                     FVALGIVEQRVKDPIIDFSLLHNRVFLSANVSLVLNFLALFAVSFMLPFYLEELRGYS
                     VIEAGLLLTPLPLTIAVIAPISGALADRIGSRWLAAGGLLIACIGLAFISQLNAQSSL
                     WDIIWRLVLTGAGQAMFQSPNNSALMGAAPRSRQGVAAGFLATGRVVGQSISVALAGA
                     IFASLGGALAGSLLVAQQFHATLSLAQVSVLQQNFAHAFQVAFLVCAGLAATGVITSL
                     VRGKEGRS"
BASE COUNT          941 a         1562 c         1324 g          808 t
ORIGIN      
        1 aacgccgtcg gcggccacag gctcgcaaac tttcgcgggc atgaaggttg ggtgcgcgcc
       61 ctcgcctggt cgcccgacgg caaacgcatc gcctccactg gcgaagacaa ggccgtgcag
      121 gtctgggacg ccgcgcgagg ccgcctcatc cacacgcatc gcggccacgc cgattgggtc
      181 aacagcctgt catggtcgct cgacggcagg cgcgtcgcct ccatcagcaa agacgcggta
      241 atccaggtct gggacgccga accctcccta cattcatcaa cgccacgttc tcaaacgtcc
      301 ggcggccctc tcgcattcca cgctaacccc tcctcgatcc acgccgtcac ctggctgccg
      361 gacgacaaac acatcgccgt tgcctgtggc gacggctcag tacaggtctg gcaggcgtga
      421 tgcatttgac atccccgcga aaccaggact atactttatg aagtgaccct gtcaaactcg
      481 aaaggaactc gtctcttgct cgtcgatagc cacgcccata tagataccgc ccgtttcaat
      541 gccgaccgcg aagccgtcat tcaagctgcc ttcgagggcg gcgtgacacg catcgtcgat
      601 cccggctgcg atctgccatc cagcgaagcc acgctagcgc tggcaaaggc acaccctggc
      661 atgatcttcg ccggggtcgg cacacacccg cacgacgcga ccacctacag tgacgaagta
      721 gggcagcgct accgcgagat ggcgcgcgag ccagaggtgg tggctatcgg tgagtgcggg
      781 ctggattatt tccgaatgct ctcgccgcgc gacgtgcagc gcgctgtctt cgtcgcccat
      841 ctcgaactgg cgcgcgcgtg cgacctgccc tgcatcatcc atgtgcgcga ctcgcacgat
      901 gacgtgatcg agctgctgcg cgcgcacggc cagggcttgc gcggcgtctt ccattgtttt
      961 tcgggcgatg tcgcgcaagc cgagcaatgc ctggagttcg agggcttcct gctctccttc
     1021 gccgggccgc tcacacgccc tggcaacgcc ttgccagagg tagcgcgcat agccccattg
     1081 gagcgcatcc tggtggagac cgatagcccc tatcttgtgc cacaacccct gcgcgcgaaa
     1141 cgcaacgagc cattatttgt ccggtatgtc gccgaaaagc tggccgagct acgcgatatg
     1201 accttcgccg aaattgccca ggttacgact gccaacgctg tccgcttgtt tggtctagga
     1261 gagatcgctc aatgaaactg ttcaacaccc tgacacaatc gcttgaagag ttcgtcccgc
     1321 tcaaagagaa aactgtacgc atctacgttt gcggcgtcac cccctatgac acgactcacc
     1381 tgggccatgc cttcacctac gtgtcctttg acacgctcat tcgctacctg gaatttcgcg
     1441 gttataccgt caactatgtg caaaatgtga ccgacatcga tgacgatatc ctgcgcaaag
     1501 cccgcgaagt cggcctggcc tgggacgagt tgggacgccg cgaaaccgag cgctacctga
     1561 acgacatgga cgcgctgaac gtgcgccgcc ccaatgtcta cgcccgcgcc acgcaagaga
     1621 cacccactat gatcgagatc gtgcggaaat tgatcgcgga cggttatgcc taccagagcg
     1681 gcggcaacgt ctatttcagc gtgaaacgcg accctgactt cggcgtgatg gcgcgcgcca
     1741 tcggcctgaa cgactacgaa tccatgctgg ccatcgctaa cgagcgcggc aacttccccg
     1801 acgatacacg caaacgcgac ccgctcgact tcgtgctgtg gcaggcgcag gcccccggcg
     1861 aaccctcatg gcccagtccc tgggggccag gacgacccgg ctggcacatc gaatgcagcg
     1921 ccatgtcgat gaaatacctc ggcccgcaga tcgacattca cggcggcggc accgacctgg
     1981 ccttccccca ccatacctgc gaaatcgcgc aatccgagca tttcaccggc aaagcgccct
     2041 tctcgcgctt ctggatgcac accggcatgg ttcaccagga tggcgaaaag atgagcaagt
     2101 ccctgggcaa cctcaccctc gtcagcgacc tgctcaaaat ctacagcgcc gacacctgcc
     2161 gcgtcaccct gctcaaccac cactaccgct acccctggga atgcttcccc gacgatctgc
     2221 aagtcgctag cgaaaccgtc gccctctttc aacagatccg ttccatcgtc ggcgatcaca
     2281 ccggcggcac ggatcacctc ctgcacgacc gcttcatcgc cgccatggac aacgacctga
     2341 acaccccgca agcgctactt ctgctgcgac aatgcgcgga gacggtcgtt gcgaatcggg
     2401 acatcaacgc gggagcagag atattgcggc tgacgaaggt actggggtta cgtctgtagg
     2461 cgcgccacac gccccttata ctgcgtcacc atcccctcgg cctcctccgc caatcgttcg
     2521 cgcagcgact ctggctccag cacgcgcgcg tgacggcccc agctcagcag ccagtgcagg
     2581 atttcgctct cctggcgaat cgtcaacgtg acgagcaggc cgtcgggcgt ctcctcctcg
     2641 gcgacgacat agtaggagcg ggcttcctgc acccagcgcg cgatatcgtg atcgaaaagc
     2701 acgcgcacca cgatttcgcg gtcgctttct ctatcacggc gcatgacgaa gttggcgggg
     2761 cgttgaaagg tcgcgggtag cagttccagg tcgtcgatac ggtcgaggcg aaagttgcga
     2821 atgccctggc gcaggtggca atagcccgtc atatgccagc cagccgggaa gtggatcagg
     2881 ccgtaggggt cggcctcgcg cctggtctgt tcgctcttgc catcctgcga gtgccgcgca
     2941 tggtaggtga aacgcacggt ggtgtgctgg atgatcgccc ggcgcagttg ttgcagcagc
     3001 gcgatgcggc gcgcattctc cggcgtctgc ggcgaagcgg ccacaaagcg gatgctgctt
     3061 tgcaggtaac gcacctcgtc gcgcagcttt tcggacagcg ccccttcgat tttccggctg
     3121 gccgactgcg ccgcctcgcg atactgtgcg tcgaagtttt gcgccatgaa atcgctgccg
     3181 agcagcagca tggtcgcctc gtcggtcgtg aagctcaacg gcggcaggaa atagccctgc
     3241 atcagcgaat agccgcgtcc gggcatggag acgatgggga cgcctgcctc gccgagcgcc
     3301 agcatatcgc gataaatggt gcgcttgctg gtctcgaagg ttgccgccag gtcttcggcg
     3361 cgctggtgtc ccttgccttg cagttccagc acaattgcta acaggcgatc agtgcggttc
     3421 atcgacgttc cttatgacct tccctctttg cctcgcacga gcgacgttat cacaccggta
     3481 gcagcaagtc cggcgcagac gagaaaagcc acctggaagg cgtgtgcgaa attttgctgc
     3541 aacacgctga cctgcgcgag tgagagcgta gcatggaact gctgcgcgac cagcaggctg
     3601 cccgccagcg ctcctcccag gctagcgaag atcgcgcccg ccagcgccac gctgatgctc
     3661 tgcccgacaa cgcggccagt cgccaggaaa cccgccgcga cgccctgccg gctgcgcgga
     3721 gccgcgccca tcaaagcgct gttgttcggc gactggaaca tggcctgccc cgcgccggtc
     3781 aacactaaac gccagatgat atcccaaagc gaactctgcg cgttcaactg gctgatgaac
     3841 gccagcccga tacaggctat caacaatccc ccggcggcca accagcgcga gccgatacga
     3901 tccgctagcg cgccgctaat tggtgcgatc accgcgatgg tcagcggcaa cggcgtcagc
     3961 agcagcccgg cctctatcac agaatagccg cgcagttctt ccaggtaaaa gggtagcatg
     4021 aagctcacgg cgaacagcgc caggaagttc agcacaagac tgacattcgc cgaaagaaaa
     4081 acgcgattat gcagcaacga aaaatcgatg atcgggtcct tgacgcgctg ctccacaatc
     4141 cccagcgcga cgaaagcgca ggcgctgacg actatcgtgc cgatcagcag cggcgaaccc
     4201 cagccccatt cctggccaaa ggaaaggccg agcgtcaggc aaaccagccc gattgccagc
     4261 agaatggctc ccagcgggtc gaaacgcccg cgctcctgcc cgctccggcg cggctccttc
     4321 aggatacgca gcgtggcaat gatgccgatg ataccgagcg gtacattcac gtagaatatc
     4381 cagcgccagg tgaaatttgc ggtgatgatg ccgcccaacg ttggtccaac gctggttccc
     4441 agcgccacaa acaccgcatt catgcccagc gcccgcccgc gttcgctggc aggaaatgcg
     4501 ctcgtcaaaa tcgccgggct gaccgccata atcagcgcgc cccccagccc ttgtagcgcg
     4561 cgcgccgcga tcaacatgcc cagcgagaac gacgcgccgc agatggccga gccggtagtg
     4621 aagatcacta gcccg
//
LOCUS       DASZAK010000995         3871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_60425, whole genome shotgun sequence.
ACCESSION   DASZAK010000995 DASZAK010000000
VERSION     DASZAK010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3871
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60425"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VGT44_17465"
     CDS             complement(<1..1295)
                     /locus_tag="VGT44_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEV2582651.1"
                     /translation="MKGSSYHQRDYAFGQLMLTLRTKLKLTQTELADLLGLRRRTVID
                     WEGGLTYPTVDHLKHFVELATERQAFPTGREAEEVRALWHAARQKVLLDEAWLDGLLS
                     HLQAPPASQAGEETAVPAHALAAPSRCGPRLDWDDALAETLFYGRQRELDLLSEWVIE
                     ERCRVVSVLGQGGIGKSALATQVMHRVAEGFEVVIWRSLRDVASCEALLDDCLQVLAP
                     QELGGISSSLESRQGLLLECLRSRRVLLVYDNLESFLEEGENSGRMRTGYEGFSRVLR
                     RIAETEHRSCLLLTSREKPSDLVPLEGNRAPVRVLRLGRLDAQACEQLLAERGVVGST
                     SEQLRLVEAYAGNPLALKIVARTIVELFEGQIAPFLEQGEVVFGGVRALLDEQYARLS
                     VVEQSVLLWLAILREPVNLQELLAVLGAPLPRPQVLEAV"
     gene            1767..2198
                     /locus_tag="VGT44_17470"
     CDS             1767..2198
                     /locus_tag="VGT44_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582652.1"
                     /translation="MIDIYAVAGTFADPKQLAIDAATTVKTIEGVPDIPMFRKNTAAF
                     VHELPAGTLANVDGDETYVRVQVLTNEGALDRNKQVAVVAQLTKLVSTAAGDATLKDR
                     TWVLLTEAKEGGWGLWGHAHTNAELVAAARDEITKLKSASS"
     gene            2284..2691
                     /locus_tag="VGT44_17475"
     CDS             2284..2691
                     /locus_tag="VGT44_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582653.1"
                     /translation="MDQHAPGHDWIALVRKNGTAEFDQAFASHPTLDASVLNGPCAGV
                     QAIAAFFAATSGGMYETIEFTHETTTGRKTYLEWQGKAFGKHVGGTTILTRNTEGLIE
                     HIQLYHSPLPVVVEFARELAKRLEAESRSGFTE"
     gene            2915..3772
                     /locus_tag="VGT44_17480"
     CDS             2915..3772
                     /locus_tag="VGT44_17480"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2582654.1"
                     /translation="MEDLTIADGPSAQVSLRILRPQHAQTPLPVIVYIHGAGWVFGSP
                     RTHDRLVRELAVGAGAAVVFPMYRLCPEVRYPTALEECYMAARWVGRHGQEHGLDAER
                     LAVAGDSAGGNMATVITLLSRERGEPDIRLQLLFYPVTDATFDTASYHQFAEGYYLRR
                     DAMMWCWDQYTRHPGERSEITASPLRASVAQLQGLPPALIITAEADVLRDEGEAYANK
                     LRQAGVRVTAVRFGGTIHDFVMLNALAYTAAARGAIALATVWLREGFSTQLTYALCTT
                     HTIWRYDKQ"
BASE COUNT          801 a         1197 c         1193 g          680 t
ORIGIN      
        1 tcgaccgctt ccagcacctg aggtcgcggc aggggtgcgc cgagcaccgc cagcagctcc
       61 tgcaggttca ccggctcgcg caggatcgcc agccacagca gcacactctg ctcgaccacc
      121 gagaggcggg cgtactgctc atcgagcagc gcgcgcaccc cgccgaagac cacctcacct
      181 tgctccagga aaggggcgat ctggccctcg aagagctcca cgatggtccg cgctacgatt
      241 ttgagggcca gaggattgcc cgcgtaggcc tcgaccagcc ggagttgctc agacgtgctg
      301 cctacgaccc ccctttcggc caacaactgc tcgcacgcct gagcatccag ccggccgagg
      361 cgcaagacac gcacgggagc ccggttgcct tccagcggca cgaggtcgct cggtttctcc
      421 cggctggtca gcagcaggca gctccgatgc tcggtctcgg caatgcggcg cagcacgcgg
      481 gagaatccct cgtagcctgt acgcatgcga ccgctgttct ccccttcctc caggaacgac
      541 tccaggttgt catagaccag caggacacgt cggctgcgca agcattccaa caggagaccc
      601 tggcggcttt cgaggctgga tgagatgccg ccgagctcct gtggagccag gacctggagg
      661 cagtcatcga gcaaggcttc gcaactggcg acatcgcgca gggagcgcca gatgaccacc
      721 tcgaagccct ccgccacgcg gtgcatcacc tgcgtcgcca gtgccgactt gccaatgcca
      781 ccctggccca gcacgctcac cacccggcag cgctcctcga ttacccactc cgagagtagg
      841 tccagttccc tctggcgccc atagaagagc gtttcggcga gtgcgtcgtc ccaatcgagg
      901 cgtggcccgc acctggaagg ggcagcgaga gcgtgggcag ggacagcggt ctcttctccg
      961 gcctgggatg ccgggggagc ctggaggtgg gacagcaacc catcaagcca ggcttcatcg
     1021 aggagcacct tctggcgggc cgcgtgccag agcgcacgca cctcctcggc ctcacgcccg
     1081 gtggggaaag cctggcgctc ggtggcgagc tccacgaagt gtttgaggtg atcgacagtc
     1141 ggataggtga ggcctccctc ccaatcgatc acggtgcggc gcctgaggcc aagcagatca
     1201 gctaattcgg tttgcgtcaa cttgagcttc gtgcgcaggg tcagcatgag ctgcccaaag
     1261 gcatagtctc gctggtggta cgaggacccc ttcatggacg ttcccttttc gtttccttcg
     1321 cacaagtgct gcgatgtgaa ggcatgtgcg gtgcaaaagt gtcgcgatgt acggtgactt
     1381 cgagttcact gcttagtata ctacaaaaca gaagcctcct cgctccgtgg tgcagaatgg
     1441 cggcgaggag tctctcggca cccaaccgca cctctcgggc tgcgcaagga cgagaggatg
     1501 caaggaacgc tcttttgcag cagacacgct gtaggcgtgg aaaatcgcct gggtgagcgc
     1561 atggatgctc atcctgagcc cgcaccacgc tatgaggtcg tgatcaaaga gccaaaaaag
     1621 ccttccaagc cgagcgggtg aatccatctg gatcatcatt ctgcacgtgt gtccgatcct
     1681 tctgaacggc tttgacctgt gaagacggga tccagtttgt ctacacatca catacgcatg
     1741 ctgacagaga ggggaacgct atgccaatga tcgatatcta cgccgttgct gggacgtttg
     1801 ccgatccgaa gcaactggcc attgacgcgg ccacgacggt gaaaacgatc gagggagtgc
     1861 ccgatatccc gatgttccgg aaaaacaccg ccgccttcgt gcacgagttg ccagcaggca
     1921 cactcgccaa tgtggatggg gacgaaacct atgtccgcgt gcaggtcctg accaacgagg
     1981 gggccctgga ccgtaacaaa caggtagcgg ttgtagcaca gctgacgaag ctcgtgagta
     2041 cggctgccgg tgacgcaaca ctgaaagacc gcacgtgggt gctcttaacc gaagcgaaag
     2101 aagggggctg gggcctgtgg ggccatgctc acacgaatgc tgaattagtg gccgcggcac
     2161 gcgatgaaat cacgaagctg aagtctgcat caagttaaca tctaatggga gagcgaaaga
     2221 acaacagcgc acctttgtag gacacaccgc ttgctcacac gctcatccga agggtattcc
     2281 atcatggatc agcacgcacc aggacacgac tggattgcac tcgtcagaaa aaatggcacc
     2341 gcggaattcg accaggcttt tgcgtcgcat ccgacgcttg acgcttcggt gctcaacggc
     2401 ccatgtgccg gcgtccaagc catcgcggct ttcttcgcgg ccacatccgg cggcatgtat
     2461 gaaaccatcg agttcacgca cgaaacaacc actggcagga aaacgtatct cgaatggcaa
     2521 ggaaaagcct ttgggaaaca tgtcggcggg accacgattc tgacgcgcaa cactgagggg
     2581 ctgattgaac atatccagct gtatcatagc cccttgccag tggttgtgga gtttgcccgc
     2641 gaactggcaa agcgtctgga ggcggaaagt agatccggct ttactgaatg aggagcgata
     2701 taaggaacgt tcttttgcag cagacacgct gtgcgtcgtc attcgtcatt gaggagccag
     2761 acacatgacc aaccggattc agctagaacc cgaggcgcaa gccttcgcag aggccgcggc
     2821 cagctcacct tggccgtcca cgcttggccc ccaacaggga cggctcgctc tcgatgaggc
     2881 gcaagcgggt ccggtcagca agttgccggt cgacatggaa gacctgacca ttgcagacgg
     2941 tcccagcgcg caggtgtcgc tgcgcatcct gcggccccag cacgcgcaga ctccgctgcc
     3001 ggtcatcgtg tatatccatg gggccgggtg ggtcttcggc agtccgcgga cgcacgaccg
     3061 gctggtgcgc gaactcgcgg tcggagccgg ggccgccgtc gtcttcccga tgtaccggct
     3121 ctgcccggag gtgagatatc cgaccgccct cgaggagtgc tacatggcgg cgcggtgggt
     3181 cggcaggcac gggcaggaac atggcctgga tgccgagcgc ctcgccgtgg cgggcgatag
     3241 cgccgggggc aatatggcga cggtgatcac gctcttgtcc cgggagcgcg gggagccgga
     3301 catccggttg cagttgctgt tctacccggt caccgacgcg accttcgata ccgcatccta
     3361 ccaccagttc gccgagggct actacctgcg gcgtgacgcc atgatgtggt gctgggatca
     3421 atacacacgc cacccgggcg agcgcagcga gatcaccgct tcgccgctgc gggccagcgt
     3481 tgcgcagttg caagggctcc cacctgcgct catcatcacg gccgaagccg acgtgctgcg
     3541 cgatgagggc gaggcctacg ccaacaagct gcggcaggcg ggggtgcgcg tcacggcggt
     3601 ccgctttggg ggcactatcc acgacttcgt gatgttgaac gccctggcat acaccgcggc
     3661 ggcgcgtgga gcgatcgcgc ttgctacggt ctggctgcgt gagggcttct cgactcagct
     3721 tacatacgcg ctttgcacta cacacaccat atggaggtat gacaagcaat aacacccgaa
     3781 caaatcaaac acgtaatcca aaggggccag tgggtgagta tcaccaccga gctgcgccca
     3841 agcaccaata aatcagccac tgtttagtct g
//
LOCUS       DASZAK010000996         3334 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_453665, whole genome shotgun sequence.
ACCESSION   DASZAK010000996 DASZAK010000000
VERSION     DASZAK010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453665"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VGT44_17485"
     CDS             <1..1167
                     /locus_tag="VGT44_17485"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEV2582655.1"
                     /translation="VLVHEFGHFITAKWAGVRVEEFGIGFPPRLVGFRKRDGGGWEVI
                     WFGGKRNAEDGAEDVGLQSPFSGTSGGITNPAVAKHHTIYSINILPIGGFVRMPGENG
                     DINDENGNYDAGSFAAKTAGKRVIILVAGVTMNVILAMVLFTIAYSLGEPTYPPAIGQ
                     VVAGSPAAQAGLKPGDTVISVNGQSVQQFTDLQSDVTNAISVDKSSSPTVPVTMVIRH
                     AGQSSDITTTVNVRKNPPSGQGPMGVVEKVVFVSSPFWEAPFKGFVHTFTVTGQFIGQ
                     IQQMVAGAVKPQLAGPVGIVKITGEVAATVPDLGWWPILSLTAILSLNLAIINILPIP
                     ALDGGRVLFVVIEVLRGGKRLKPEREGIINFVGMAILLTLMVVITVSDVIHWGS"
     gene            1188..2423
                     /gene="ispG"
                     /locus_tag="VGT44_17490"
                     /gene_synonym="gcpE"
     CDS             1188..2423
                     /gene="ispG"
                     /locus_tag="VGT44_17490"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEV2582656.1"
                     /translation="MRRKTRQIHVGGLPIGGDAPIAVQSMTTTYTRDVESTVAQIHRL
                     EEVGCEIVRVAVPEMEDALAIGEIKKRIHIPLVADIHYKHVLALEAIRQGVDCVRVNP
                     GNLINGRQSLEEIVALCKERGTAMRIGVNSGSIDALDQRGQMQRIQVRLRDDGVLERS
                     DPAEARRKERELLAERMVNKALDYIGWCEEMDFHEIKVSLKSSTPLTAVEAYRRFSER
                     SDYPLHLGITEAGTLVTGAVKSSVGLGLLLASGIGDTIRVSLSAEPEEEIPVAYEILR
                     SLELRNRGVTFVSCPSCGRVEIDVIGVANEVEKRLSKVQTPIHVAVMGCVVNGPGESR
                     DADVGLAGGKGRGVIFRKGEVVKTVPEDQFLDAVLKEVASLLPEREAELVYPKGGIHE
                     TAGVHRTRDEGIGERVARS"
     gene            complement(2475..2548)
                     /locus_tag="VGT44_17495"
     tRNA            complement(2475..2548)
                     /locus_tag="VGT44_17495"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2512..2514),aa:Val,seq:gac)
     gene            2747..3163
                     /locus_tag="VGT44_17500"
     CDS             2747..3163
                     /locus_tag="VGT44_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582657.1"
                     /translation="MMTSTEKLIYKLQVVAFLLSTAGWGWAAGNFTPSGAPFINPLLH
                     TIPVVLLLIQGLLFFRAPGEGRPTRGAIVFISVMVLLNIVTVAVLIVLGATNPDPNAV
                     GVKTLEDWFPTVIMLMGDFLWLATLIPARLARVQAA"
BASE COUNT          702 a          951 c          998 g          683 t
ORIGIN      
        1 gtgctggtgc atgagttcgg gcattttatc acggcgaaat gggcgggggt gcgcgtcgag
       61 gagttcggca tcggtttccc tccgcgcctg gtcggattcc gcaaacgcga tggcggcggc
      121 tgggaagtga tctggttcgg cggcaagcgc aatgccgagg atggcgcgga agatgtaggc
      181 ttacagtcgc cctttagcgg cacgagtggc ggcataacga atcctgctgt ggcgaaacac
      241 cacaccatct attccatcaa tatcctgccc atcggcggtt ttgtacggat gccgggcgaa
      301 aatggcgata tcaacgacga aaatggcaac tacgacgcgg gcagctttgc cgcgaagacc
      361 gccgggaagc gcgtcattat cctggtcgcg ggcgtcacga tgaacgtcat cctggccatg
      421 gtgctgttca ccatcgccta tagcctgggc gagccaacct atccgcccgc catcggccag
      481 gtcgtggcag gctctccggc tgctcaggcg ggtctcaagc cgggtgatac cgtcatttcg
      541 gttaatggcc agtccgtgca gcaatttacc gatttgcaaa gcgatgtaac gaatgccatc
      601 agcgtggata aaagctcctc accgacggtc ccggtcacaa tggtgatacg ccacgccggg
      661 cagagcagcg acattaccac gacggtgaac gtgcgcaaga acccgccgtc aggccaggga
      721 ccgatgggtg tcgtggagaa ggtcgtgttt gtcagctcgc cgttctggga agcgcccttc
      781 aaggggtttg tgcatacctt tactgtcacc ggccagttta tcggccagat ccaacagatg
      841 gtggcgggcg cggtcaagcc gcagctggcc ggaccggtgg gcatcgtcaa gataacgggc
      901 gaggtggccg cgaccgtgcc ggacctgggc tggtggccga ttttgagcct gacggccatc
      961 ttgagcctca acctggccat catcaacatc ctgccgatcc ccgctctaga tggtgggcgc
     1021 gtcttattcg tagtgatcga ggtgctgcgc ggcgggaaac gactcaagcc cgaacgcgaa
     1081 ggcatcatca actttgtcgg gatggcaata ttattgaccc tcatggttgt tattactgtg
     1141 agtgatgtca ttcattgggg cagttagctc ctggggcaac aaacattatg cgaaggaaaa
     1201 cacgacagat acatgtaggc ggcctgccca tcggcggcga cgcaccgatt gcggtgcagt
     1261 ccatgacgac gacctatacc cgcgacgtcg agagtacggt ggcgcagatt caccggctgg
     1321 aagaagtcgg ctgtgagatt gtgcgcgtgg ctgtgcctga gatggaggat gcgctggcga
     1381 ttggcgagat caaaaagcgc attcatatcc cgctggtggc cgatattcat tataaacacg
     1441 tcctggcgct ggaagccatt cgccaggggg tcgattgcgt gcgggtcaat cccggcaacc
     1501 tcatcaatgg ccgtcaatcg ctcgaagaga tcgtcgcgct gtgcaaagag cgcggcaccg
     1561 ccatgcgcat cggcgtcaac tccggctcga tcgacgcgct cgaccagcgc ggccagatgc
     1621 agcgcatcca ggtcaggctg cgcgatgatg gcgtgttgga gcgcagcgac ccggcggagg
     1681 cgcgccgcaa ggaacgcgag ctgctggccg agcgcatggt caacaaggcg ctcgactaca
     1741 tcggctggtg cgaggagatg gacttccacg agatcaaggt ctcgctgaaa tcttccacgc
     1801 cgctgaccgc cgtcgaagcc tatcgccgtt tctcggagcg ttccgactat cccttgcacc
     1861 tcggcatcac cgaggccggg acgcttgtca ccggcgcggt caagtcctcc gtgggcctgg
     1921 gtctgctgct cgcaagcggc atcggcgata cgatccgcgt cagcctgagc gcagagccgg
     1981 aggaggagat cccggtggct tacgaaatcc tgcgctcgct tgaactgcgc aatcgcggcg
     2041 tgaccttcgt ctcctgcccc tcctgcgggc gcgtggagat cgacgtaatc ggcgtcgcca
     2101 acgaggtcga gaagcgcctc tccaaagtgc agacgcccat ccatgtcgcc gtgatgggct
     2161 gcgtcgtcaa cggccccggc gagtcgcgcg acgccgatgt cggcctggcg ggcggcaagg
     2221 gtcgcggcgt gatcttccgc aaaggcgaag tggtcaagac cgtccccgaa gatcaattcc
     2281 tcgacgccgt gttgaaagag gtcgccagcc tgctccccga acgcgaggcg gagctggtct
     2341 acccgaaagg cggcattcac gagacggcag gcgtgcatcg cacccgcgac gagggcattg
     2401 gcgagcgcgt ggcacgttcg tagaactgca caataagaaa agcggcttcg caatgaagcc
     2461 gcttttttga cgagtgggcg atacagaact cgaatctgtg acctctacga tgtcaacgta
     2521 gcgctctaac caactgagct aatcgccctt gatgggcata ataatagcat tgctatacgg
     2581 cattgtcaag cgaaaatttt gccaatccca tatgacaaat gtcatggcat cctcatgata
     2641 gtcttcacta ccgcctcagc cgccgctcac ctatacttgg catataagca aagtgaacaa
     2701 gcgagttcac acgaaggcaa taaaagcaga aagaaggaaa actatcatga tgaccagtac
     2761 agagaaactc atctacaaac tgcaagtggt ggcgttctta ttgtcaacgg ccggctgggg
     2821 atgggctgcc gggaatttta ccccgtccgg tgctccattc atcaatccgc tcctccatac
     2881 tatccctgtg gtactgttgt tgatccaggg cttactgttt ttccgcgcgc ctggtgaagg
     2941 tcgtccaacc aggggagcaa tcgtttttat cagcgtaatg gtcctgttga acatcgtcac
     3001 cgtcgccgta ttgatcgtcc tcggcgcgac caatcctgac cctaacgcgg tcggcgtaaa
     3061 gacccttgag gactggttcc cgaccgtcat catgctgatg ggcgacttcc tatggctggc
     3121 gacgctcatc cccgctcgac tggcgcgcgt acaggccgcg taatctcagc aatctcgtgt
     3181 agtccgtagg gcgaaaagaa tagatggaaa ctcttctcgc tcctactcta ccataagagg
     3241 agtacagcta tgaatatcaa ggcatttatt caacgtcatg ctgttgtgat ctactttgcc
     3301 cttgttctct tgatctccta tggaagcttt gcaa
//
LOCUS       DASZAK010000997         1501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_238173, whole genome shotgun sequence.
ACCESSION   DASZAK010000997 DASZAK010000000
VERSION     DASZAK010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238173"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1501
                     /locus_tag="VGT44_17505"
     CDS             <1..>1501
                     /locus_tag="VGT44_17505"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEV2582658.1"
                     /translation="YIYLKMVYHLSEEARTGLLAFRIPDVFRDRIFDYQVAAVKIAAH
                     HLNRRGGVLIGDVVGLGKTLMATALARIMQDDYGLETLVLCPRNLTGMWQDYLDEYRL
                     VGKVVSVTNVQNELADLRRFRVVIIDESHNLRNREGRRYKIIKDYIEKNESKCILLTA
                     TPYNKTYLDLSAQLRLFLSDEQGKDLGIRPERLIANLGESRFSAQYQCPVRSLTAFEH
                     SEYTDDWRDLMRLYLVRRTRSFIQDYYASADAETGRKYLLFGDGRRSYFPVRVPHTVK
                     FTVEDSRDSRDPYAILYSDSVVEAVNTLSLPRYGLANYIAKKPGVPPTPNERRQLESL
                     SKAGKRLMGFCRTNLFKRLESGGPAFLQSLERHVLRNFVYLHAIEHNLDLPIGTQDAE
                     LLDPGVSDEDDNALASDEMDENGFEPDTENSAAASIAPSLDLPLDEVAFRGRAAQVYA
                     QYAGALRKRFKWLRHSLFTANLKRDLLQDVQSLLAVLRMCGTWDAARDA"
BASE COUNT          312 a          474 c          427 g          288 t
ORIGIN      
        1 attacatcta tctcaaaatg gtctaccatc tctcggagga ggcgcgcacc ggcctgctgg
       61 catttcgcat ccccgatgtc ttccgggatc gcatcttcga ctaccaggtc gcggcggtca
      121 agatcgcggc gcatcacctc aatcggcgcg gcggtgtgct gatcggagat gtcgtcggcc
      181 tgggcaagac gctgatggcg acagccctgg cgcgtatcat gcaggacgac tacggcctgg
      241 aaacgctcgt gttgtgtccc aggaacctca ccggcatgtg gcaggactac cttgacgagt
      301 atcgcctcgt ggggaaagtg gtcagcgtca ccaacgtgca gaacgaactg gccgacctgc
      361 gccgctttcg cgtcgttatc atcgacgaga gccacaacct gcgcaaccgt gaaggccgtc
      421 gctacaagat catcaaggac tatatcgaga agaacgagag caagtgcatc ctgctcaccg
      481 ccacgcccta caacaaaacc tatctcgacc tctcggccca gctgcgcctg ttcctgtccg
      541 atgagcaggg caaagacctg ggcattcgcc cggaacgcct gatcgccaac ctgggcgaga
      601 gccgcttcag cgcgcagtat caatgccctg tgcgctcgct gaccgccttt gaacacagtg
      661 agtataccga tgactggcgc gacctgatgc gcctctacct tgtgcggcgc acgcgcagct
      721 tcatccagga ctactacgcc agcgccgatg ccgaaacggg gcgtaagtac ctgctcttcg
      781 gcgatggccg ccgctcgtat ttccccgtgc gcgtgccgca tacggtaaag ttcacagttg
      841 aagactcccg cgattcccgc gacccctatg ccattctcta ttccgatagc gtggtcgagg
      901 ctgtgaatac actgagcctg ccgcgctacg gcctcgcaaa ttacatcgcg aaaaagcccg
      961 gcgtgccgcc tactcccaac gagcgacgcc agcttgagag cctttccaaa gcgggcaagc
     1021 ggctaatggg cttttgccgc acgaatctct tcaagcgcct ggagagcggc ggcccggcct
     1081 tcctgcaatc gctggaacgc cacgtgctgc gcaacttcgt ttacttacat gccatcgagc
     1141 ataatcttga tctacccatc ggcacgcaag atgccgaatt gctcgatccg ggcgtgagcg
     1201 acgaagacga caatgccctc gcatccgacg aaatggatga gaatgggttt gaaccggata
     1261 cggagaacag cgcagcggcc agcatcgccc cgtcgctcga tttgccgtta gacgaggttg
     1321 cctttcgcgg gcgagcggcc caggtgtacg cgcagtatgc cggggcgctg cgcaaacgct
     1381 tcaagtggct gcgacattca ctcttcacgg caaacctgaa acgcgatctc ttgcaagatg
     1441 tgcagtcgct cctggctgta ctgaggatgt gtggcacatg ggatgccgcc agggacgcca
     1501 a
//
LOCUS       DASZAK010000998         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_167880, whole genome shotgun sequence.
ACCESSION   DASZAK010000998 DASZAK010000000
VERSION     DASZAK010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167880"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="VGT44_17510"
     CDS             <1..879
                     /locus_tag="VGT44_17510"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV2582659.1"
                     /translation="KARKQFGDAVTIVPGEINDEEQVRRAVDGANIVYHFAGRLYHPS
                     TPAVLYYQTHVEGTRNLLAACEGQSQLRRIVHCSTTGVHGVTGRTPAAEDAPFAPTNP
                     YEETKLQGELLALQAHQERGLPVSVARPGLVYGPGDLHLLGFFASIKKGLPCLIDRGK
                     ALIHPIYIDDMTEAFLLCAERPEAIGRSYNLAGERHVSFRELVSAIARALGKSSPGGS
                     IPLWLANVASDTFALLPGFKGEDAPLTRSRVKFLTNSRVYDISRARDELGFAPRVELD
                     EGMKQTAQWYQKHEYL"
     gene            949..2592
                     /locus_tag="VGT44_17515"
     CDS             949..2592
                     /locus_tag="VGT44_17515"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV2582660.1"
                     /translation="MQFDPDELPPPIRPRPARPRLVGMEQNQASPYNTPVTPQSANRT
                     TSDDVSAQPTSALDSMQLGHTPPAQSVPKLPPLRPRPERPAFFMPDTADISTQSTQHL
                     MQLSGMMRAVKVPTQNSEGPVSPLPTMPMTNDFEDGYWPYGIQQTGALPVVNLYGREP
                     FGRTLPLAAVSAGAAVPLTPPIAAKPRSAWRAALGSPVVKVVVGLLIGVGLLFLVAHF
                     VDLPATIAILRQNLTTPRGIIFALLSGVAFLIAFSIRGVRWKLFINPIGKISTFKAIQ
                     LFLVGIFLNFLLPIRGGEVAKSLMLKRIADIPISKSLPTVAMDKALDLMPALFIMAIV
                     PLLGVKMDIKLWIVLALVGGLLIGLIFFIALAAWKRDAAIHLLQRLTGILPKSIGSKI
                     EGFATGFVDSLLAGASQPRIFIPAVLLTVVAVIFDGLFAMLAFWTVGFPISFGTAIFG
                     YTVYNMFYILPTPPGQVGSNEAIGLLVFSGLLHLPADKVTAMFVFSHPYAALIMTITG
                     MACLSALGLKLSSAMKVQTGNEGALETQEKTVQHAPARV"
     gene            complement(2601..>3033)
                     /locus_tag="VGT44_17520"
     CDS             complement(2601..>3033)
                     /locus_tag="VGT44_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2582661.1"
                     /translation="GLGHIIHYVQGSGEALPFADGSFSAIVCLDVLEHVGNVKKTIGE
                     IARVLAPGGVFVFDTINRTLLARAVLIWYGEHVPIGGLKPGVHQYEKFIKPRELTALL
                     VGSGLQVRELTGFMPRGFKQGRFSMRPGWFKGVAYVGYATK"
BASE COUNT          590 a          983 c          832 g          628 t
ORIGIN      
        1 aaggcgcgca agcaattcgg cgacgccgtc acgattgttc ccggcgaaat caacgatgaa
       61 gagcaggtgc ggcgcgcggt ggatggcgcg aatatcgtct atcatttcgc aggacggctc
      121 taccatccca gcactccggc agtgctctac taccagacgc acgtcgaagg aacgcgcaac
      181 ttgctcgccg cctgcgaggg tcagagccag ctgcgccgca tcgtgcattg cagtacgacc
      241 ggcgtgcatg gcgtcaccgg tagaacgccc gccgccgagg acgcgccctt cgctcccacc
      301 aatccctatg aagaaaccaa gctccaaggc gaactactcg cgctccaggc gcatcaagag
      361 cgcggattgc ccgtcagcgt ggcgcgcccc ggcctggtct acggtcctgg cgacctccac
      421 ctgctgggct tctttgcttc gatcaagaaa ggcttgccct gcctgatcga cagaggaaaa
      481 gcgttgattc atccgatata tattgatgat atgaccgagg cattcttgct ctgcgcggaa
      541 cgtccagaag cgatcgggcg cagctacaat cttgccggtg agcggcacgt ctcatttcgc
      601 gagctggtct cggccattgc gcgcgcgctt ggcaagtcct cgccgggagg atcgataccc
      661 ttgtggctcg ccaacgtcgc ctcggacacg tttgcgctgc tgccaggctt caaaggcgaa
      721 gacgcgccac tcacgcgcag ccgcgtcaag ttcctgacca acagccgcgt ctacgacatc
      781 agccgcgcgc gcgatgagtt ggggttcgcg ccgcgggttg aactggacga gggcatgaag
      841 caaaccgccc aatggtacca aaagcatgaa tatctttaac tactgttaat attctttcat
      901 ttaactcgtt gatatgtaag aatacagtca ccgaatcgag ggtttcgtat gcagtttgat
      961 ccagatgagc tgccgccgcc tattcgcccg cgtcccgcgc ggccacggct ggttggtatg
     1021 gagcaaaatc aagccagtcc ctacaatacg ccggttacgc cgcaaagcgc gaaccgcacc
     1081 acgtcagacg atgtgagcgc gcaaccgaca tctgcccttg attcgatgca gttgggacat
     1141 acccctcccg cgcaatccgt gccgaagctt cccccccttc gaccgaggcc cgaacgcccg
     1201 gcatttttta tgcccgatac cgccgatatc agtacgcaga gtacgcagca ccttatgcag
     1261 ctcagcggca tgatgcgcgc cgtcaaggta cccacccaaa atagcgaggg accagtatcg
     1321 ccactcccca caatgcccat gaccaatgac ttcgaagatg gctactggcc gtatggcatt
     1381 cagcaaaccg gcgcgttgcc ggtcgtgaac ctgtatggac gcgaaccctt cgggcgcacg
     1441 ctgccgcttg ccgctgtgtc cgccggggct gctgtgcctc tgaccccacc catcgcggcc
     1501 aaaccgcgat cggcatggag ggccgcgctg ggttcgccgg tagtgaaagt cgtggttggc
     1561 ttgctcatcg gcgtgggctt gctgtttctc gtagcgcatt tcgtcgattt gccggcgaca
     1621 atcgccatac tgcgccagaa tctgactaca cccaggggca tcatttttgc cctgctttcc
     1681 ggcgtggctt ttcttatagc cttttccatt cgcggtgtgc gctggaagct gttcatcaat
     1741 cccatcggta agatcagcac attcaaagcc atccaactct tcctggtggg catttttctc
     1801 aacttcctgc tgcccattcg cggcggcgag gttgccaaga gcctgatgct caagcgcatt
     1861 gccgacatcc ccatcagcaa atcgctgccg acggtcgcca tggacaaggc gctggacttg
     1921 atgcccgcgc tcttcatcat ggccattgtg ccgttgttgg gggtgaaaat ggatatcaag
     1981 ctctggatcg tgctagccct ggtaggcggc ctgctcatcg gcctgatctt cttcatcgcc
     2041 ctggccgcct ggaagcgcga cgccgccatt cacctgctgc agcgcttgac cggtatcctg
     2101 cccaaatcga tcggcagcaa gattgaggga ttcgccaccg gtttcgtcga ttcgctgctc
     2161 gcaggcgcga gccagccgcg catcttcatc cccgcggtgc tgctgacggt cgtcgcggtg
     2221 atcttcgatg gcctgttcgc catgctcgcc ttctggacgg ttggcttccc gatctcgttc
     2281 ggcacggcca tcttcggcta taccgtctac aacatgttct acatcctgcc cacaccgccc
     2341 ggacaggtcg gcagcaatga ggccattggt ctgctggtct tctctggctt gctgcatctg
     2401 ccagccgata aagtgaccgc tatgttcgtc ttctcacatc cctatgccgc cctcatcatg
     2461 accatcaccg gcatggcctg cctcagcgcg ctcggcctga agctttccag cgccatgaag
     2521 gttcagaccg gcaacgaagg cgcgctagag acgcaggaaa agacagtaca gcacgcacca
     2581 gctcgcgtgt agggtcatat tcatttcgtt gcgtatccca cgtatgccac gcccttgaac
     2641 cagccgggac gcatgctgaa acgaccctgt ttgaagccgc gcggcatgaa accggtcagc
     2701 tcgcgcacct gaagcccgct tcctaccagc agagcagtca gttcgcgcgg cttgatgaac
     2761 ttctcatact ggtggacgcc cggcttcagg ccgccgattg ggacatgctc gccgtaccag
     2821 atcagcacgg cgcgcgccag cagcgtacgg ttgatcgtat cgaacacaaa gacgccgccg
     2881 ggcgcgagaa cccgcgcgat ctccccgatg gtcttcttca cgttccctac atgttccagc
     2941 acatccaggc agacaatcgc cgagaaactg ccatcggcga agggcagcgc ctcgccgctg
     3001 ccctgtacgt aatgaatgat gtgtcccaat ccc
//
LOCUS       DASZAK010000999         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_524471, whole genome shotgun sequence.
ACCESSION   DASZAK010000999 DASZAK010000000
VERSION     DASZAK010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524471"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VGT44_17525"
     CDS             complement(<1..241)
                     /locus_tag="VGT44_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582662.1"
                     /translation="MSQHTTETEHGKTATDASPSGRSRLRRLITALTVVAFLAIGLVA
                     LYALSLILTAVVVLMLSALLAYFSYPLVQFLQRRLT"
     gene            complement(326..1171)
                     /locus_tag="VGT44_17530"
     CDS             complement(326..1171)
                     /locus_tag="VGT44_17530"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2582663.1"
                     /translation="MGTNEDRPFSLEANTDEHEPEQPQTPTGGTGHVAVRNLETQKRR
                     RKAAAFLLLGDALQLLLFGALALSIHIHPLLPIDVVITRSFQQNQAPWLRISMLVISY
                     PGSSFLLPTLVLLSVAIFWSLGDRLEAVFVAGLSACSLLLNFLLKVLVHRPRPSGGLV
                     HIIVKSAGYSFPSGHVMAYIAYWGLLFAFGVILFQGRHWWRSTLLVTSAAFVVLVGPS
                     RIYLGDHWASDVIGAYLIGGVLWGLAVGLYLPLKERGVLQTPRALARMREKKSLRSFP
                     TKRWF"
     gene            complement(1273..1821)
                     /locus_tag="VGT44_17535"
     CDS             complement(1273..1821)
                     /locus_tag="VGT44_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HEV2582664.1"
                     /translation="MQQLLSNFRNMFLFRGIAAIVFGVLTLVWPKLTVLALVLLFGIF
                     AIISGITVVAAALQNREEHGWGVLLFEGILWLLVGVVALIWPGVTALSFLYLLAAWAI
                     ITGILELVAPLSFPMSGGRGVLMALSGLVSIVFGIVIAVQPASGLLAVTWLIGIYAIV
                     FGILHLVAYFEARSVSQALSHS"
BASE COUNT          506 a          561 c          556 g          385 t
ORIGIN      
        1 gtgtgaggcg gcgttgtaaa aactgcacaa gtggatagct gaagtaggcg agcagtgctg
       61 aaagcatcag tacaacgacc gccgtcagga tcaatgagag cgcgtacagc gcaaccaatc
      121 caatggctag aaacgccaca accgttaatg cggtgatcaa tctcctcagc cgagagcgac
      181 cagaagggga cgcatcagtt gctgtcttcc cgtgttcagt ttcagtcgta tgttgagaca
      241 tgtccggtac ttcctattcg atgcgctcct gggtatacac tatttcgctt agccagtaac
      301 aaacgttcct ggaggtttca cgagttcaaa accaacgctt cgtcggaaat gatctcaagg
      361 acttcttttc cctcatgcgt gcgagtgccc tcggcgtctg cagcacaccg cgctctttga
      421 gcggaagata gagacccacg gcgaggcccc acaagacccc accgatcagg taggccccga
      481 tgacatcgct ggcccagtgg tctccaagat aaatacggga aggcccgacc agaaccacga
      541 aagcggccga ggtcaccagt agagtagaac gccaccaatg cctaccctgg aagaggatga
      601 caccgaaggc gaagagcagt ccccaataag caatgtaagc catcacatgg ccgctgggga
      661 agctgtaccc ggcgctcttc acaataatat gcactaggcc ccctgacggg cgcggccggt
      721 gaacgaggac cttgagcagg aagttgagta gcaggctgca ggccgacagc cccgcgacga
      781 acaccgcttc taagcggtct cccagcgacc agaagatcgc tactgacagc agcaccaggg
      841 taggcagcag gaacgaactc cccggatagc tgatgacgag catgctgatg cgcagccagg
      901 gagcctggtt ctgctgaaag gagcgggtaa tcaccacatc gatgggcagg agaggatgga
      961 tgtggatcga tagcgccagc gctccaaaca gcagaagctg cagcgcatct cccagaagca
     1021 ggaaagcggc tgccttcctg cggcgctttt gcgtttccag attgcgtacc gctacgtgtc
     1081 cggtgcctcc agttggtgtc tgcggctgtt ccggttcgtg ttcgtctgtg ttggcctcaa
     1141 gagagaaagg ccggtcttca ttggtcccca tcgcaaattc cctcttcgat ccttcgctcc
     1201 cgatcaccct ccatgagggt ggatcgggag cagtaagctc ttgaaagatg gtgttcaggc
     1261 cgcatgggat gtctaagagt gtgatagagc ctgcgacacg gaacgtgctt caaagtacgc
     1321 cacaaggtgc aggatcccaa acacaatggc gtatatgccg atcaaccagg tcacggcgag
     1381 cagccccgat gcgggttgga cggcgataac aatgccaaag acgatcgaga ccaacccgga
     1441 taacgccatc aacaccccgc gcccaccgct catcgggaac gagagcggtg cgaccagctc
     1501 aagaatgccg gtaatgatgg cccaggccgc cagcaggtaa aggaaagaga gcgcggtgac
     1561 gcccggccag atcagggcaa ccactcccac caggagccac aggattccct cgaagagaag
     1621 cactccccat ccgtgctctt ccctattttg cagtgcagcc gccaccactg tgatgccgct
     1681 gatgatggca aagatgccga acaagagcac cagggcgagc acagtcagtt ttggccagac
     1741 cagagttaag acgccgaaca caattgcggc gatgccgcga aacaaaaaca tgttacgaaa
     1801 gttacttaga agttgctgca ttgtatgtct ccttattgtt gtaattgttg tttgacaacg
     1861 aaaaaacgat agatcggtgt tgttcgacta ttctcttcaa aagcctacgg gtttcgcgct
     1921 acaagatacg tgcaacatgc actccttaca aatgattcga ctccattttg gcttgacgca
     1981 gttgctcggg agtgatgagc gctccttc
//
LOCUS       DASZAK010001000         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_701075, whole genome shotgun sequence.
ACCESSION   DASZAK010001000 DASZAK010000000
VERSION     DASZAK010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701075"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..>2570
                     /locus_tag="VGT44_17540"
     CDS             385..>2570
                     /locus_tag="VGT44_17540"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV2582665.1"
                     /translation="MFADITGSTPLADRLDPEDMRAILAGYFNLMTEQIRRHGGTVEK
                     YIGDAVVAIFGAPIAHEDDPDRAIRAALDMQAALARFNQRRQAQDPAVTRLQMRIGIN
                     TGEVAAPGAQFSRRDFLITGDAVNVAARLQQVAAPDTILVGERTYLATREVFDFRPLA
                     PLTLKGKPEPVRAWVVEGFRSRTSAIIQHPRGIEGLEAPLVGRDLELTLIHATYARVQ
                     AERHSHLITLLGAPGIGKSRLMREFIAREEELAKWASRDSRLSSPRVLQGRCPPYGEG
                     ITYWPLVEILRTLLKVQGGESREALEKRFNEFVRDTLAQAKRSEDPAEVASAIIRSIG
                     RGLGGDGSEQSFAERREIQRSTRSKSTEQGGPQAVVLRAWRALFESIAQQEPLILVID
                     DLQWADEALLGLLEYLTDRITDVPILFLCPARPDFLERRRDWGGGRRNFTTIVLDALT
                     SEESLDLITGLLGTHDLPDVLYYTIQNRAEGNPFYIEEIMRMLIDQGMLVNQNGCWRI
                     GEQNEAILSELASPATPPDDTLIDQHYILPLPRLPDTIQGVLAARIDLLSQVEKQVLQ
                     YAAIIGRTFWLAGIIELATDLDSDTVLKTLHVLIQHEFIIEAEKQVRTPVEQDLVYSF
                     KHVLIRDVVYNNIPRIRRSQKHAQLALWLEEKTAGNTEAFAELLAYHYRQALANWSAS
                     LLVTTAGTSEITGQVPGSSTQPITLTRQELNRRSIIYLTMAGDQAY"
BASE COUNT          614 a          741 c          706 g          509 t
ORIGIN      
        1 acaatagtcc cacgtcagaa aagcacgttt gttgtacaat agatgagagc gaatgcgtta
       61 ttttcgaccg acgcggggac cagtgaccaa aaagacagga tagatatgcg ctgcccgaac
      121 tgccagacga tcaacccacc aaatgcaaaa ttttgccttg aatgcgggaa tcgcctggtc
      181 atctgcccaa actgcggcac agtcaacttg ccgaatgcaa aattctgcat tgaatgcgga
      241 atcgcgttaa cgccaacacc cagaggcacg aacccgcctc cagcgatgca agccgccaga
      301 agcgagaggc taccatactc atcagaactg gatggcagca aaagcaggct tacagcacca
      361 gaggaaaggc gtatcgttac cattatgttc gccgatatca ccggttcgac gcccctggcg
      421 gataggctgg accctgaaga tatgcgagct atcctggcgg gatatttcaa tctgatgact
      481 gagcaaatac gcaggcacgg cggtactgtc gagaaataca tcggtgacgc ggtggtggct
      541 attttcggcg caccaattgc gcacgaagat gatcccgacc gcgcgattcg agcagcgctg
      601 gatatgcagg cagcgcttgc caggttcaac cagcgaaggc aggcgcaaga cccggcggtt
      661 acgcgcctgc aaatgcgtat tggcataaac accggcgagg tcgccgctcc tggcgcccag
      721 ttcagcaggc gcgatttcct catcaccggg gatgccgtta acgtcgccgc ccgcctgcaa
      781 caggtcgctg cacccgatac gatcctggtg ggagaacgaa catacctggc gacccgcgag
      841 gtatttgatt ttcgcccgct cgccccattg acgctcaaag gcaagcctga acctgttcgc
      901 gcctgggtcg tagagggctt ccgatctcgt acttcggcca tcatccagca tccgcgtggc
      961 atcgagggac tcgaagcccc actggtaggg cgcgacctgg aacttacctt gatccatgcg
     1021 acctacgcac gtgttcaggc cgagcggcat tcccatctga ttacactgct gggtgctccg
     1081 ggcattggta aatcccgtct gatgcgcgaa ttcatcgcgc gcgaagaaga attagcgaaa
     1141 tgggcctctc gcgacagcag gttgagtagc cccagggtct tgcaaggtcg ttgtccacct
     1201 tacggggaag gtatcaccta ctggcccctg gttgagatac tacgcacctt attgaaagtc
     1261 cagggaggag aaagcagaga ggcattagaa aaacgcttta acgagtttgt gcgggatacg
     1321 ctcgcgcaag caaaacgcag cgaggacccg gcagaggtcg ccagcgctat catccgcagc
     1381 atcgggcgcg ggcttggagg cgatggcagc gagcagagtt tcgccgagcg acgggaaatt
     1441 caacggagca cacgcagcaa aagtacagaa cagggaggtc cccaggcagt ggtattgcgc
     1501 gcatggcgcg ccctcttcga gtcaatagca cagcaagagc cgcttattct tgtgatcgac
     1561 gatctgcagt gggcagacga ggcgctgctc ggtctgcttg aatacctgac cgaccgcatc
     1621 accgatgtgc ctatcctttt tctctgtccc gcccggccag acttcctcga gcggcgacgt
     1681 gattggggcg gcggacggcg caatttcacg acgattgtgc tggatgccct tacgagcgag
     1741 gaaagccttg atctcatcac cgggctattg gggacgcacg accttcccga cgtgctctac
     1801 tatactatcc agaacagagc cgagggcaac cctttctata ttgaggaaat catgcgtatg
     1861 ctgatcgacc agggcatgct ggtcaatcaa aatggctgct ggcgcatcgg tgagcagaac
     1921 gaggctatct taagcgaact tgccagtccc gcgacgcccc ctgatgatac gctgattgac
     1981 cagcattata tactgccact tccccgcctg cccgacacta tacagggcgt gcttgccgcg
     2041 cgcatcgacc tgctgagcca ggttgagaag caggtgctcc agtacgctgc catcatcgga
     2101 cgtaccttct ggctggctgg tattatcgaa ctggccacgg accttgacag cgatactgtt
     2161 ctcaagacgc ttcatgtatt gatccaacat gaatttatca ttgaagctga gaagcaggta
     2221 cgcactcccg ttgagcagga cctcgtatac agcttcaagc atgtgctgat acgcgacgta
     2281 gtctacaaca atatcccgcg tattcgccgt tctcaaaagc atgcccaact cgcgctctgg
     2341 ttggaagaaa agacagccgg caataccgaa gcgttcgcag aactgctcgc ctatcattac
     2401 cggcaagcgc tggctaactg gtcagccagc ctgctggtaa ctactgccgg tacaagcgaa
     2461 ataaccggcc aggtgccagg ctcatcaacg cagcctataa ccttaactcg ccaggaactc
     2521 aatcgccgct cgattatcta cctcacaatg gctggggacc aggcctatca
//
LOCUS       DASZAK010001001         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_487410, whole genome shotgun sequence.
ACCESSION   DASZAK010001001 DASZAK010000000
VERSION     DASZAK010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487410"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VGT44_17545"
     CDS             <1..252
                     /locus_tag="VGT44_17545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582666.1"
                     /translation="EALVAQAEALAAQPTARHRSRILLNQITAPTLVIAGDNDPLAFR
                     PEVLAASIANAQTLLLPGDHLSVVRLPDFATAIVEFLAS"
     gene            complement(738..1601)
                     /locus_tag="VGT44_17550"
     CDS             complement(738..1601)
                     /locus_tag="VGT44_17550"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2582667.1"
                     /translation="MESRAGELEKLTVDVGSYSFTALAAGPPDGPLALLLHGWPEFAS
                     SWTEVLLALGSAGYRAVAFDQRGYSTGARPAEVEQYAPDHLVADALAVADAQGARRFH
                     LVGHDWGGMVAWALASAHPERLLSLAVLATPHPVALQQASAEDADQYHRLDYVRFFRR
                     PDGAAERSLLADHAARLRAAYLGRVPQHLVSENIRRLSEPGALTATLNWYRAVDDDLT
                     VPAGRIAVPTLYIWGSEDAALGRNAATRTAGFIDGPYRFEILEGASHWLPEEAPGVVV
                     PLLLKHLSAQG"
     gene            complement(1916..2389)
                     /locus_tag="VGT44_17555"
     CDS             complement(1916..2389)
                     /locus_tag="VGT44_17555"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2582668.1"
                     /translation="MKAPLDYSRGPEKTWVYGALRVRDGKELTRCAASRNSKNYIARL
                     SDVEAQNPTGDIFIITDNLSSHNSLETRTWLEAHPRLHHVFIPTGACWLNLQEGWWRL
                     FRRDALAGQSFATADEIEQATRVATAQLNQRAKPWIWGRPPKTRRHLRRLFCYRI"
BASE COUNT          489 a          799 c          763 g          583 t
ORIGIN      
        1 gaagctctgg ttgctcaggc agaagctctg gctgctcagc caactgcacg gcatagaagt
       61 cgcattctct tgaaccagat tacagcccca acgttggtca tcgcgggaga caatgatcct
      121 ctggccttcc ggccagaggt actcgctgcc tccattgcca atgcacagac gctgctcctg
      181 cccggtgacc atctcagtgt tgtcagattg cccgacttcg ctaccgcaat cgttgaattt
      241 ctcgcctcgt agcactgttt gaggtgagtg tgcattgttg atgggcggac ccctatttcc
      301 cttgacagga aggatgtatc atcaggatat tcttggtctg ctgaatggcg aggatacaaa
      361 cacgaagcaa taataatgcc ggtgattgag cagtttctca ctgcttctgc tgcataggag
      421 tgtttggaac cgagttcagg gtggggcttt gccaccaccc tgatgagtat tcattctata
      481 ttcctactgc tacgttcctt aaatgtggta agaaagcgga ttagtctgtg gaggcaattc
      541 atgctgattc cttcatgttt ttgtcatcat tgcatttgac gaacatagca ctattgtgtg
      601 tttcctcttc ttcgtaccac acagtcctcc agactgcgtc ctggcctcgt tcgaggtact
      661 cgtcttcgac gccactcctg gagtgaatgc ctctctctcg atgtgagccg ccggccgccg
      721 gtcccccacc cctctcctca gccttgcgcc gagaggtgtt tcaggaggag cgggacgacc
      781 accccaggcg cctcttctgg cagccagtgg ctggctcctt cgaggatctc gaaccggtag
      841 ggtccatcaa tgaatcccgc cgtccgcgtg gcagcgttgc gccccagcgc cgcgtcctcg
      901 ctaccccaga tatagagcgt gggtacggcg atgcggcccg cagggacggt gagatcgtcg
      961 tcgaccgccc gataccagtt cagggtggcg gtcagagccc ccggttccga cagccgccgg
     1021 atgttctcgc tcacaaggtg ctgcggtacc cggccgaggt acgcggctcg caggcgggcg
     1081 gcatgatccg ccagcaggga ccgctccgcg gcaccgtccg ggcgccggaa gaagcggacg
     1141 tagtcgagcc gatgatactg atccgcatcc tcagcggacg cctgctgcag ggcgaccgga
     1201 tgcggggtcg cgagcacggc gagggagagc aagcgctccg ggtgggccga ggccagcgcc
     1261 caggccacca tgccgcccca gtcgtgtccc accaggtgga atcgccgtgc cccttgagca
     1321 tcggccacgg cgagcgcgtc ggccaccagg tggtccggcg cgtactgctc cacctcggcc
     1381 ggccgcgccc cggtcgagta tccacgctgg tcaaaggcga cggcgcggta gcccgccgag
     1441 cccaacgcca ggagcacctc ggtccacgag ctggcaaact caggccatcc gtgcagcagc
     1501 agggccaggg ggccatcggg cggtccggcc gccagagccg tgaacgagta cgaaccgaca
     1561 tcgacggtca gcttctccaa ctcgcccgca cgggactcca ttggattctc ctcctctttt
     1621 ggggcctgcc catatggtac aggaccattc gttctgtttg tgttctccat actcgccttt
     1681 cctttccttt tcatcatcat ttctgtcgca gagcgacatc ggccttctcc tggcgctcat
     1741 ggcgccgcaa gatcgtcgca cctcgtcctc tcggccgcta gtatacacgg caaacgttat
     1801 gaaaccgtga tgagcagcgt tatgaacctc ttcacctcta caggagtcag gagcagggga
     1861 acaacgcttt gttcgtttgc tcatcacatc ggttgtccta ctattgctgt gttccttaaa
     1921 tccggtaaca aaagagacgt cgcaaatggc gtcgcgtttt aggaggccgt ccccagatcc
     1981 agggtttggc tcgctgatta agttgcgccg tggcgacacg agtggcttgc tcaatctcat
     2041 cagcagtggc aaagctctgt cccgctaaag catcacgccg gaagagacgc caccaccctt
     2101 cctgcaaatt gagccaacag gctcctgttg gaatgaagac gtgatgcagc cgcggatgtg
     2161 cttccagcca agtgcgcgtc tccaagctgt tgtggctcga gagattatcg gtaataatga
     2221 aaatgtcacc tgtcggattc tgcgcttcaa cgtcggaaag cctcgcgatg tagttcttgg
     2281 agttgcgcga ggccgcacag cgcgtcagtt ccttgccatc gcgcacgcgc agcgctccgt
     2341 acacccaggt cttctcgggg ccgcgactgt aatccagcgg cgctttgatg cggggacccg
     2401 tgggagacca gcctagagcc ggtggaaagg tgcggggaac tgctggctaa agcatcgtcg
     2461 gtgcagacgg tcgtcgaccc ctcgggcggc tgcgtgtagt gagtgacgac cgccgttctt
     2521 tctattttat cactacttga ggaacagagc attacactta ttctgttgac gccggttctg
     2581 gtccatggtg tgagcgggtt tctgttactc caagtgtgcg ctgaagtgcc cgtt
//
LOCUS       DASZAK010001002        16930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_60594, whole genome shotgun sequence.
ACCESSION   DASZAK010001002 DASZAK010000000
VERSION     DASZAK010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 16930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16930
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60594"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /gene="hypD"
                     /locus_tag="VGT44_17560"
     CDS             complement(<1..1052)
                     /gene="hypD"
                     /locus_tag="VGT44_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEV2582669.1"
                     /translation="MNMKYVDEYRDPELAKRISAEIAKLTGSHSLKFMEVCGGHTHTI
                     YKHGIEDVLPPTIDLVHGPGCPVCVLPMGRIDDAIAIARMDGVIFTTFGDMMRVPGSN
                     GSLLDAKADGADVRFVYSPLDAYKLARQNPDRQVVFFAIGFETTAPSTAVTLLRAKAD
                     GLRNFSVFCNHVTIIPAIKAILDSPDLRLDGFVGPGHVSMVIGMRPYNFIARDHRKPV
                     VIAGFEPLDIIQSVYMLAKQIAEGRSEVENQYARVVRSEGNVKALQAMAQTMELRPFF
                     EWRGLGFITHSALKLRPEYADWDAELRYEVPGLRVADPKACQCGEVLKGVIKPWECKV
                     FGTACTPETPIGTCMVS"
     gene            complement(1052..1744)
                     /locus_tag="VGT44_17565"
     CDS             complement(1052..1744)
                     /locus_tag="VGT44_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV2582670.1"
                     /translation="MHNEEGSEYDRLFYPYLFEGGKASIEDVLAQVRHSTLEKCREVI
                     ALRRATHESSGEQIVAAGQAMARAFQKGATLLAFGNGGSTTDAQDLVADLLTPPFSDW
                     RPLPAIALTNDIAVVTGVGNDVGFDNIYVRQLIALGRPGDIAFGISTSGNSTNVLVAF
                     EQAKKQGLLTIGLSGYDGGKMIRSAAVDVCINAASDHIPRIQEAHATAYHALLEVIHA
                     HLTHALAMKGDP"
     gene            complement(1737..2060)
                     /locus_tag="VGT44_17570"
     CDS             complement(1737..2060)
                     /locus_tag="VGT44_17570"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEV2582671.1"
                     /translation="MSNLQGKPVAMPVSDALRLELVGGDSCVLDAEGHCITCSDEALP
                     ARVLHIDQEQSIALVEINNESVEVDITLVDEVMVGSWLLVHGGVGIATMVNEEGQPID
                     EASDA"
     gene            complement(2057..2671)
                     /locus_tag="VGT44_17575"
     CDS             complement(2057..2671)
                     /locus_tag="VGT44_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV2582672.1"
                     /translation="MAQENEQSYLKTLVAARFEQSMSAPEAFFESSAGLIAEACWAMA
                     RRFHQGGRLLAFGNGASATDAQHVSVEFVHPVIVGKRALPALALTNDSATISGHITGK
                     EYALLFARQLDVLARSQDIAMGFSVDGYCENVVLALDRSQQKGLLTLGLTGSEGGAMR
                     HAGLDYCFVVPSHDPFVIQETHETLYHILWELVHIFFEHEGLLT"
     gene            complement(2684..2956)
                     /locus_tag="VGT44_17580"
     CDS             complement(2684..2956)
                     /locus_tag="VGT44_17580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEV2582673.1"
                     /translation="MCLGIPGQIIDIVDDANSIAKVDVSGVRRNVSVALVRPDGIGPG
                     DWVLIHVGFAMSKIDEEEARETMKALEYMGQAYADELDMLHSSHIE"
     gene            complement(2975..5326)
                     /gene="hypF"
                     /locus_tag="VGT44_17585"
     CDS             complement(2975..5326)
                     /gene="hypF"
                     /locus_tag="VGT44_17585"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HEV2582674.1"
                     /translation="MSSAAESTIQRRRIIVQGIVQGVGFRPFVYGQARKRGLVGFVRN
                     DSQGVTIEIEGADAALDGFQSALQDQTPPLARIDSLTTELIPTCHEQVFTITHSQQGA
                     ERLALISPDTATCDDCLRELFDPADRRHHYPFINCTNCGPRFTIVQDVPYDREKTTMR
                     VFPMCPDCQAEYDDPGNRRFHAQPNACPVCGPQVSFVEWRIHVPGVDPIGEAAQRLSA
                     GQILAIKGLGGYHLACDACNSEAVQRLRQRKHREARPFALMVLDLETARQLCQVSESE
                     AALLQTYRRPIVLLARQAECPVAPSVAPNCTTLGIMLPYTPLHHLLLRAFAGAVAADR
                     PVAFVMTSGNVSDEPIAYREDDARQRLYSIADGMLTHNRDIHMRCDDPVVRIIGGSEQ
                     FFRRSRGYAPEPIQLTLAAPLPILACGAHLKNTFCLGKGQRAFLSHHIGDLENLETLT
                     SFREGIAHFQRLFDIYPEAIAFDLHPEYLATKYALDSPIPQKMGVQHHHAHIASVLAE
                     HGLDGPVIGVAADGTGYGTDGAVWGCEVMVADLAAFERLAHLAYVPLPGGEQAVRQPW
                     RMAAAYLSQAYGEDFLELDIPFVQQIDRLKWRALGQMMARNLNSPPTSSLGRLFDAVA
                     ALLGVRGVAHYEGQAAIELEALAQSGQHGLSSADSVSTYPFIIGEKGEQTPAQINVRP
                     VIRSIVIDIQRGVPAREIALRFHRTVAELLATACLEARDSTGLDAVALSGGVFQNRIL
                     LEQLLKRLEAMAFRVYINRRVPPNDGGLALGQLAVAAARLRSV"
     gene            complement(5523..6188)
                     /gene="hypB"
                     /locus_tag="VGT44_17590"
     CDS             complement(5523..6188)
                     /gene="hypB"
                     /locus_tag="VGT44_17590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEV2582675.1"
                     /translation="MVKVTIAQNILSANDNVAQEVQQRLAAYGVRAVNIMSAPGAGKT
                     TLLERTIERLRGQLRIGVIEGDVETTADAERIEAAGAQTVQINTRGACHLEAHMIRDA
                     LAEIDAAELDLLVIENIGNLVCPAAWNLGEEQRVVVVSTTEGDDKPAKYPQMFAISQV
                     MVVNKLDLLPFVDYDLEKVKRYALTINPRLRIFEVSCRTGDGLGAWCEWLLSFAHELQ
                     AHL"
     gene            complement(6201..6566)
                     /gene="hypA"
                     /locus_tag="VGT44_17595"
     CDS             complement(6201..6566)
                     /gene="hypA"
                     /locus_tag="VGT44_17595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEV2582676.1"
                     /translation="MHELSIAQSIAEAVEIKAVECSAARVKRVRLKIGEASGIVADTL
                     AFCFEMIASQTPLLEGAHLSIDRVPHRAFCRCCAGEFDVKNFIARCPKCGEWSAEVVS
                     GNELEILDMEIETKSSDAL"
     gene            complement(6886..7437)
                     /locus_tag="VGT44_17600"
     CDS             complement(6886..7437)
                     /locus_tag="VGT44_17600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEV2582677.1"
                     /translation="MNAPSSPQKKVRRILIACIGNIFMGDDGFGVEVAQRLASRKSGR
                     YPQGVEVFDFGIRGVDLAYTLLEDYDTLVLVDAIARGGTPGTLYLIEPDLSEISPENA
                     VEVARVGLDAHSMDPLKVLAFARTLGARPIRTLLIGCEPSVIHANEDYLDMHMGLSQP
                     VLAAVDEAVKMLDSLIEQLLASE"
     gene            complement(7434..7784)
                     /locus_tag="VGT44_17605"
     CDS             complement(7434..7784)
                     /locus_tag="VGT44_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582678.1"
                     /translation="MSNVNWNFPGEDALPPESVLVAGSEVKAGDRVILHPGRRSVERQ
                     GSLTDAFDIMLDGKTARVEIIEQDFENRLYLVVTLDDDPGREPWDERMMPGHRFFFFP
                     EEVEPLGGSARREV"
     gene            complement(7781..8812)
                     /locus_tag="VGT44_17610"
     CDS             complement(7781..8812)
                     /locus_tag="VGT44_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582679.1"
                     /translation="MSLEQVKEIANAVLYEGYLLYPYRQSAIKNRQRWTIGVVYPQEY
                     SEANGGIEPWEMQTECLVVGIASTSLDVYVRFLHLLLRTVEQPQTHAGVSNTGDWSLA
                     RRFADEPREEGVEREIAAQNLSLSELVAHPYTIDFTIPGERMVEIAEESPGMVMRKQY
                     PIEGTISIAAKQLDMGLFKLSVRIENRTPETGTVATLRNAIMLHSFISTHTILQARRG
                     AFVSQLDPPEPVYDAAKACVNLRTWPVLVGSEGDNDTMLSSPIILYDYPQIAPESPGS
                     LFDGTEIDEILTLRIMTLTDEEKEEMRQGDERVREILERTENLSPEAFKKLHGTIRSL
                     RPVAEEGPA"
     gene            complement(8809..9459)
                     /locus_tag="VGT44_17615"
     CDS             complement(8809..9459)
                     /locus_tag="VGT44_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HEV2582680.1"
                     /translation="MPDLSFEIIDAEVPAYAAVPTLTFKLRVTNAHEEERIHSVSLRS
                     QVQIAVTRRRYSPEAQERLLELFGEPKRWRDTLHPLLWTNTSSVIPQFDGSLVTDLPI
                     TCTYDFEVASTKYFDALEDGEIPLAFMFSGTIFYEGEEGNLQVAQIPWSKESFYRLPV
                     ATWKEMIARYYPNSAWIRLHKDVFDQLYHYKATHSLPRWEDVITRLLQSSDEEVYP"
     gene            complement(9452..10147)
                     /locus_tag="VGT44_17620"
     CDS             complement(9452..10147)
                     /locus_tag="VGT44_17620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HEV2582681.1"
                     /translation="MPAPYLSNDGDNGSSIAPVDVASMPALRGFAQRRREAQMEHCEL
                     CSEGIPASHSHLLNLSNRALVCVCQACSLLFSEQGAGAGKYLLVPRRYQALVDFQMAD
                     DQWDDLMIPVNMAFIFRSGGAKPVMAYYPSPAGATESLLDLEGWQALVNNNPVLNTLA
                     PDVEALLINRIKDVRAYYIVPIDACYHLVGLIRLSWRGLGGGKEVWEAVAGYFQDVQR
                     KAQPVFVKGVDHA"
     gene            complement(10153..10779)
                     /locus_tag="VGT44_17625"
     CDS             complement(10153..10779)
                     /locus_tag="VGT44_17625"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEV2582682.1"
                     /translation="MKQDAQTQSRAERIETLVQQVSSFSNIHARETTEELVQCILDMY
                     GECLARILELSIQADAAGFPLVRSFTRDDLIGSLLILHGLHPVDLETRVMRALDEVRP
                     QLVLHGGNVEFLKIEDNVAYLRLNGSCHGCPSSTVTLKQLIEGAVRKAAPDLERLDVD
                     GVADGTTAINPDSPPGITCGVPITFVPPRRNREGAQLAVPGKSLKNGR"
     gene            complement(10894..12684)
                     /locus_tag="VGT44_17630"
     CDS             complement(10894..12684)
                     /locus_tag="VGT44_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEV2582683.1"
                     /translation="MATEVKRGETPVQPPQLVEMSWDPITRSVGSLGIYTKIDFKNRR
                     VAECHSTSSIFRGYSLFMQGKDPRDSHFITSRICGICGDNHATCSVYTQNMAYGVKPP
                     NLGEWILNLGEAAEYMFDHNIFQENLVAVDYCEQMVKETNPGVLELANKTEAPHAREH
                     GYRTIGDIMRAFNPFTGELYLEGLQVSRYTREKFCLMEGRHVHPSTLYAGGVGTVATV
                     QLFTDYLVRLMRYVEFMKKMVPLHDDLFDFFYEALPGYEQVGYRRTLIGCWGSWQDPD
                     VCDYDYRTMDEWGRAMFVTPGVIVDNELVTTSLVDINLGIRILLGHSYYDDWDNERTF
                     VDRDPLGNPVDKRHPWNQTTIPKPQKRNFDDKYSWTMSPRWYDKRTGDYLALDTGGGA
                     IARLWTTALAGLVHLPGLIEATGHSVKITLPKSATKPAVDFEWQIPKWSNAIERNRAR
                     TYFQAYSAAVALHCVDKALAEIHAGHTKTWNEFTVPKDAIGCGFHEAVRGVLSHHMVI
                     ENGKIANYHPYPPTPWNGSVRDIYGTPGPYEDAVQNTPIFEDNGPDKFKGIDIMRAVR
                     SFDPCLPCGVHMYLGDGKELQVVHSPTFGV"
     gene            complement(12735..13766)
                     /locus_tag="VGT44_17635"
     CDS             complement(12735..13766)
                     /locus_tag="VGT44_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HEV2582684.1"
                     /translation="MAARSRVEDVHIVWITAGLGCDGDTVSITAATQPSIEDVLLGAI
                     PGLPRVHLHNPVLAFELGGDSFMTWWYAAERGELDPFVLVVEGSIPNEKIKSEGYWAA
                     LGTDPTTGQPITTNEWIDRLSPKALAVIAIGTCATYGGIHAMSGNPTGAMGLLDYLGW
                     NWKTGAGIPIICVPGCPVQPDNFMETVLYLLYQAAGMAPMMPLDDAHRPTWLFGNTVH
                     EGCDRAAYYETGDFATEYGSPKCLVKLGCWGPVVQCNVPKRGWMAGIGGCPNVGGICI
                     GCTMPGFPDKFMPFMDEPPGAKFSSRAIGLYGSIIRPLRKLTQNTANKEPSWRHKGRE
                     LMSGYTGRW"
     gene            complement(13873..14334)
                     /locus_tag="VGT44_17640"
     CDS             complement(13873..14334)
                     /locus_tag="VGT44_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HEV2582685.1"
                     /translation="MSLEFSGVQTLPVPREEVWDYLLNTKKVASCAPGFESIEELAEE
                     RWKAIVGAAIGPIKSRFMMDVTRSGMEEPERMMLTGRGNAPGSTVEVSGAMRLIAATP
                     DSTRMDWTATLDVSGTLAKVGVFMLKGTVEKLTGQFFECLKKSIQAHYGRW"
     gene            complement(14358..14702)
                     /locus_tag="VGT44_17645"
     CDS             complement(14358..14702)
                     /locus_tag="VGT44_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV2582686.1"
                     /translation="MKGLVTGLFLGVGVGLLFAPMRGEEMRRLLNEQFLGTRGNELLK
                     QFAPLLATDLSQVRKSLDSLTRFALSSVKADEATLTGLARLAVDRLMKYRVPVSLNDL
                     VDLASVVMKKAS"
     gene            complement(14948..15649)
                     /locus_tag="VGT44_17650"
     CDS             complement(14948..15649)
                     /locus_tag="VGT44_17650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2582687.1"
                     /translation="MSISPQRQHRHGSARLVIADDHELARAGLRTMLIGQHGMELLAE
                     AKNGREALEFCRRLHPDLALIDVRMPDMDGLATCRSIKQESPSTSVILVTMHENPHYL
                     VEALRAGASAYVLKDVTQRELLATIRRVLRGESVLDPDIVMRVLGHMTGETSLQSELL
                     AVQLSPREREVLQLLAQGLTNREIARALTVSASTIKIHVEHILAKLGVSDRTQAAVRA
                     IELGLFQSTYNANRI"
     gene            complement(15639..16748)
                     /locus_tag="VGT44_17655"
     CDS             complement(15639..16748)
                     /locus_tag="VGT44_17655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEV2582688.1"
                     /translation="MRESEERFRNIFAEVPIGMAIVDLEGHVLQVNKAFCEMLEYDEQ
                     ELIGFNLSALSHPDDVGADMLLAGKALSGEMSSYKVEKRYLKKNQETLWADLTATILR
                     NQRGDAIYGLVMLENIIERKRAKLLEEERHHVAYELHDGLAQVAVSAHQHLQALASHY
                     RPRSPQARQELNRALELAQLSVREARRLIAGLRPTVLDDFGLTTALRLHVEAQRNEGW
                     TIIFDEALGLERLPRTIETTLFGVAQEALTNVRKHSHTTQVRLSLALQDASVRLEVQD
                     WGCGFEPRTLFKVYRPGEHVGIRAMQERVELLGGHLLVTSQAGVGTLVVAEVPVLSSN
                     GKDQCDADSTVIKETTPLLFRSHLAETERNEYDEH"
BASE COUNT         3600 a         5037 c         4696 g         3597 t
ORIGIN      
        1 gataccatgc aggtgccgat gggcgtctcc ggcgtgcagg ccgtgccgaa taccttgcac
       61 tcccagggct tgatgacgcc cttcagcact tcaccgcact ggcaggcttt cgggtcggcc
      121 acgcgcagcc caggcacttc atagcgcagc tcggcatccc aatcggcata ctctggccgc
      181 agcttcagcg cgctgtgcgt gatgaagccc agcccgcgcc actcgaagaa tgggcgcagt
      241 tccatcgtct gtgccatggc ctgcagcgcc ttgacgttgc cttcagaccg caccacgcgc
      301 gcgtactggt tttccacctc cgagcggccc tcggcgatct gcttcgctag catatagacc
      361 gactggatga tatcgagtgg ctcaaaaccc gcgatcacaa ccggctttcg gtggtcgcgc
      421 gcgatgaagt tgtacggcct catgccgatt accatactga cgtggccggg accgacaaat
      481 ccatcgaggc gcaggtcggg cgaatcgagg atggccttga tggccggaat gatggtgaca
      541 tgattgcaga agaccgagaa attcctgagg ccgtcagcct ttgcgcgcag cagtgtcacg
      601 gcggtcgaag gcgcggtcgt ctcgaaaccg atggcgaaga agaccacctg ccggtcggga
      661 ttctgtcgag cgagcttata ggcatccaac ggcgagtaga cgaaacgcac gtcggctccg
      721 tcggccttcg cgtccagcag gctgccgttt gaacccggca cgcgcatcat atcgccgaaa
      781 gtcgtgaaga tcaccccatc catgcgcgca atggcgatag catcgtcgat acgccccatc
      841 ggcagcacgc agaccgggca gcctggtcca tgcacgagat cgatagtggg cggcagcacg
      901 tcctcgatgc catgtttgta gatggtatgc gtatgcccgc cgcatacctc cataaacttg
      961 agcgagtgcg agccggtgag tttcgcaatt tcagcggaga tgcgtttcgc cagttctgga
     1021 tcgcggtact cgtcaacata cttcatgttc attagggatc tcctttcatt gcaagcgcgt
     1081 gggtcaaatg cgcgtggatg acttcgagca gcgcgtgata ggctgttgcg tgcgcctcct
     1141 ggatgcgcgg aatatgatcg cttgcggcat tgatgcacac atctactgcc gccgagcgta
     1201 tcatcttgcc gccgtcatag ccgctcaacc caatcgtgag cagaccctgc ttctttgcct
     1261 gttcaaaggc caccagcacg ttggttgaat tgccgctcgt cgatatgccg aaagcaatat
     1321 cgcccggtcg cccaagcgcg atgagctggc gcacgtagat attgtcgaat ccgacatcgt
     1381 taccgacccc cgtcaccact gcaatatcgt tggttagcgc aatcgccgga agtggacgcc
     1441 agtcgctgaa gggcggcgtc agcaggtcgg ccaccaggtc ctgcgcatcc gtcgtactgc
     1501 caccattgcc aaaggccagt agcgttgccc ctttttggaa ggcgcgcgcc atggcctggc
     1561 ctgccgcaac gatctgctcg ccagacgact catgcgtcgc ccttcgcagc gcgatcacct
     1621 cccggcactt ttcgagcgtg gaatgccgca cctgcgccag tacatcctcg atactggcct
     1681 tacctccttc aaagagataa ggataaaaca ggcggtcgta ttcagacccc tcttcgttat
     1741 gcatcgcttg cctcgtcaat cggttgacct tcttcgttaa ccattgtagc gattcctaca
     1801 ccaccatgca ccagcagcca gcttcccacc ataacctcat ccacaagtgt gatatcgact
     1861 tctactgatt cattattgat ctccaccagc gcaatactct gttcctgatc gatatgcagc
     1921 accctggcgg gcagcgcctc gtcggagcaa gtgatacaat gcccctccgc atcaagcaca
     1981 caactatctc cgccgaccag ttcaagtcga agagcatcgc tgacgggcat tgcgactggc
     2041 ttcccttgca aattgctcat gtaagtagcc cttcatgctc aaagaaaata tgcaccagtt
     2101 cccataaaat atggtacagc gtttcgtgcg tctcctgaat tacaaacggg tcgtgtgacg
     2161 ggacgacaaa gcagtaatcg agtcctgcgt gtcgcattgc tccaccctcg ctaccggtca
     2221 ggccaagcgt cagtagccct ttttgttgcg atctgtccag cgccaggacg acattttcgc
     2281 agtacccatc gacggaaaag cccatcgcga tatcctgcga gcgggcgagt acgtcaagtt
     2341 ggcgcgcgaa caacagggcg tattctttcc cggttatatg gccgctaatc gtcgcgctgt
     2401 cgttggtcag ggccagcgca ggcagggcgc gcttgccgac gatgaccgga tgcacgaact
     2461 cgaccgagac atgctgggca tcggtggccg atgcgccgtt gccaaaggcg agcaggcgcc
     2521 cgccctggtg aaagcgacgc gccatcgccc agcacgcctc cgcaatcaat cccgccgatg
     2581 attcaaagaa tgcctccggc gcggacatgc tctgctcaaa gcgcgccgcg accagcgttt
     2641 tcaggtaaga ttgctcgttt tcttgcgcca tagtccatcc tcgtcattca atatgactcg
     2701 aatgtaacat gtccaactca tcggcatatg cctgccccat atattccagc gccttcatgg
     2761 tttcgcgcgc ctcctcttca tcgatcttac tcatggcgaa gccgacgtga atcaagaccc
     2821 aatcgcccgg cccaatgcca tcgggacgca ccagcgccac gctgacgttg cgcctcacac
     2881 cggatacatc gacctttgca atcgagttag cgtcgtccac gatatcaatg atctgacccg
     2941 gaattccaag acacataggt gcctccttct gttgctaaac actgcgcagc cgcgcggcag
     3001 caactgcgag ctggccgagg gcgaggccgc catcgttagg cggcacacgc cggttgatat
     3061 acacccggaa cgccatagcc tccaggcgtt tcaacaattg ttcgagtaat attctattct
     3121 ggaagacacc gccgctgagc gctaccgcat ccagtcctgt gctatccctc gcctcaagac
     3181 aggcggtcgc gagcaattcc gcgaccgtgc gatgaaaacg cagcgcgatc tcgcgagccg
     3241 gaacccctcg ctgaatatcg atcacaatag atcggatcac cggcctgacg ttgatttgtg
     3301 caggtgtctg ttcccccttc tctccaatga tgaatggata ggtagagacg gaatccgcgc
     3361 ttgataaccc atgctgacct gattgcgcca gcgcctccag ctcgatggct gcctgtcctt
     3421 cgtagtgcgc cacgccgcgc actcccagca gcgcggctac tgcgtcgaac aagcgtccca
     3481 ggctggaggt cggcggactg ttgaggttgc gcgccatcat ctgtccaagc gcgcgccact
     3541 tcaagcgatc tatctgctgc acgaacggga tatccagctc caggaagtct tcgccgtaag
     3601 cctgggaaag gtaggcagcc gccatacgcc agggctggcg cacggcctgt tcgccgccgg
     3661 gcagcggcac gtaggccagg tgcgccagcc gttcaaaggc tgcgaggtct gccaccatta
     3721 cctcgcagcc ccagaccgcg ccatcggttc cgtagccggt gccatcggct gcgacgccaa
     3781 tcacagggcc gtccaagcca tgttcggcca ggacgctggc gatatgggca tggtgatgct
     3841 ggacgcccat tttctgtggg ataggggagt ctagcgcgta tttggtcgcc aggtattcgg
     3901 gatgcaggtc gaatgctatc gcctccgggt agatgtcgaa caggcgctgg aagtgcgcga
     3961 taccttcacg aaacgaggtc agcgtttcca ggttttccag gtcgccgatg tggtgactca
     4021 gaaacgcgcg ctgccccttt cccaggcaga aagtgttttt gagatgggcg ccgcaggcca
     4081 gtatcggtag cggcgctgct aatgtgagtt gaatcggttc aggcgcgtat ccacgcgagc
     4141 ggcggaaaaa ctgctcgctg ccgccgatga tgcgcactac cgggtcatcg cagcgcatat
     4201 gaatgtcgcg gttatgcgtc agcataccat ccgctataga atacaggcgt tggcgggcgt
     4261 catcttcccg gtaggcaatc ggctcatcgc tcacgttgcc gctggtcatc acaaacgcca
     4321 ccgggcgatc tgctgcgacg gcccctgcga aggcgcgcaa tagcagatga tgcagaggag
     4381 tatagggcag catgatgccc agcgtcgtgc agttcggcgc aacggatggc gcgacggggc
     4441 attcggcctg gcgggcgagc agcacgatgg ggcgacgata cgtttgcagc agcgccgcct
     4501 cgctttcgct tacctggcag agctggcgag ctgtttccag gtcgaggacc atcagcgcga
     4561 aaggtctggc ctcgcgatgc ttgcgctgcc gcaggcgctg tacggcctcg ctattgcagg
     4621 catcacaggc aaggtgatag cctcctaaac ccttgattgc gaggatttgc ccggcagaga
     4681 gacgttgggc ggcctcgccg atcggatcaa cgccagggac gtgaatgcgc cattcgacga
     4741 aactcacctg ggggccgcag accgggcagg cgttgggctg ggcgtgaaag cggcgattgc
     4801 cggggtcgtc gtattctgcc tgacaatcgg gacacatcgg gaagacacgc atggttgtct
     4861 tttcgcggtc gtagggaacg tcctgcacaa tggtgaagcg cggaccgcag ttggtacagt
     4921 tgatgaaagg ataatgatgg cgccggtcgg ctggatcgaa caactcgcgc aggcagtcgt
     4981 cgcaggtggc agtgtcgggc gaaatgagag caaggcgttc agcgccctgc tggctgtgag
     5041 tgatggtaaa tacctgctcg tggcaggtgg gaatcagttc ggttgtgagg gagtcgatgc
     5101 gcgccagggg cggcgtttga tcttgcagcg cggactggaa accatccagc gcggcgtcag
     5161 cgccctcgat ttcgatggtg acgccctggc tatcattgcg cacaaaaccg accaggcccc
     5221 gcttccgcgc ctgcccatag acgaaaggcc ggaagccgac accctgtaca atgccctgta
     5281 caatgatgcg acgacgctgg attgtcgatt ccgccgcgga tgacatacac cgttccttac
     5341 tagacacaac tctgaaggtg tcgattgatc gcacaccacc gatgatgaag tttcacaacc
     5401 aaagccggtc ctgtcatcct gagcgcagcg aaggatctca gtacccggcg agtcagatcc
     5461 ttcgctgcgc tcaggatgac aacctatgat cgatcctggt cgcaaaacct cattatcgac
     5521 acctacaaat gggcttgaag ctcgtgggcg aatgatagga gccattcgca ccacgcaccc
     5581 aggccatcgc ccgtccggca gcttacctcg aagatgcgca gacggggatt gatggtcagg
     5641 gcgtatcgtt tcactttttc aaggtcgtaa tcgacaaagg gcagcaggtc gagcttattg
     5701 acaaccatga cctgcgagat ggcgaacatt tgcggatact tggctggttt atcatcgcct
     5761 tcggttgtgc tgacaacgac gacacgctgt tcttcgccca ggttccaggc ggcgggacag
     5821 accaggttgc cgatgttttc gatcaccagc aggtccagtt ccgccgcgtc aatctcagcg
     5881 agggcgtcgc ggatcatgtg cgcttccagg tggcacgccc cccgcgtgtt gatctgaacg
     5941 gtctgcgccc ctgccgcctc gatgcgttct gcgtcggcgg tggtctcgac atcgccctcg
     6001 atgacgccga tgcgtagctg cccgcgcagt cgttcaattg tacgctccag tagcgtcgtc
     6061 ttcccagcgc cgggcgcgct catgatgttg acggcgcgca ctccatacgc ggccagccgc
     6121 tgctggacct cctgggcaac gttgtcattg gccgacagta tgttttgagc gatggtaact
     6181 ttgaccacga tccgcccctc tcagagagca tcactagact tcgtttcgat ttccatatca
     6241 aggatttcga gttcgttgcc ggacacaacc tccgcggacc actccccgca ttttggacac
     6301 cgtgcgatga aattcttaac atcgaactcc ccggcgcaac agcggcagaa ggcgcgatgc
     6361 ggtacgcggt caatcgataa atgcgcgcct tccaggagcg gggtctggct ggcaatcatc
     6421 tcaaagcaga atgccagcgt atcggccacg ataccgctgg cctcgccaat cttgagtcgc
     6481 acgcgcttca cacgcgccgc gctgcattcg accgctttga tctcaacagc ttcagcgata
     6541 ctttgagcaa ttgagagttc atgcataacc gaccctgttc caggtagaga cgacgcgcat
     6601 cacatcgagc gtattcgccg atagcggcga atattgggca acatgaataa aaaggcgatc
     6661 aggccaacaa cgatcagtac aagcaagatg acaagcacta caatcaaagc agccatagtc
     6721 tcgttccttt ccctgcacgg gttgcagaag ctgaaccctt gcgcgcctac atcgagcgca
     6781 atttcaggta ccgctctaaa tcaggaagcg actgcaagaa aatccacacg cccgccgcag
     6841 ccagacccat aacgattaga aacttgaaca tggtattttc cctttctatt cgctcgcgag
     6901 cagttgctcg atgagcgagt cgagcatctt caccgcctca tcgacggctg ccagcaccgg
     6961 ctggctcaag cccatgtgca tatccagata atcttcattc gcgtggatca ccgaaggttc
     7021 gcagccgatc agcaaagtac ggatggggcg agcgcccaac gttctcgcaa acgccagaac
     7081 tttcagcggg tccatactgt gcgcatccag tcctacgcgc gcaacctcta ccgcattctc
     7141 cgggctgatc tccgacaagt ccggctcgat cagatacagc gttccgggcg taccaccgcg
     7201 cgcgatggca tccacaagca cgagcgtgtc gtaatcttcg agcaacgtat acgccaggtc
     7261 aacgccgcga ataccgaaat cgaagacctc gactccctgc gggtatcgcc cggatttgcg
     7321 gctcgccagt cgttgcgcca cttcaacgcc gaaaccgtcg tcacccatga agatattgcc
     7381 aatgcaggcg atcaggatgc gcctgacctt cttttgcgga gacgagggcg cattcatact
     7441 tctctcctcg ccgaacctcc cagcggttcc acctcctccg ggaagaagaa aaagcggtgg
     7501 ccgggcatca tgcgttcgtc ccatggctcc cgcccagggt catcatccag ggttacaaca
     7561 agatacagcc ggttctcaaa atcctgctcg atgatctcga ctctcgcggt cttcccatcg
     7621 agcataatgt caaaggcgtc ggtcagagac ccttgccgtt ccacactccg gcggccagga
     7681 tgcagtatga cgcgatctcc ggccttgacc tcactgccgg ccaccagcac cgattcggga
     7741 ggtagagcat cctcgcctgg aaagttccag ttgacgttgc tcatgctggc ccctcctcag
     7801 cgaccgggcg caggctgcga atggtgccat gcagcttctt aaatgcttcg ggcgaaaggt
     7861 tctcggtgcg ttccagtatc tcgcgcactc gttcgtcgcc ctgccgcatt tcttccttct
     7921 cttcgtcggt cagcgtcatg atgcgcagcg tcagaatttc atcgatctca gtgccgtcga
     7981 aaagcgagcc gggactttct ggcgcgatct gcgggtaatc gtacaggatg atgggcgacg
     8041 atagcatggt atcattgtcg ccctcgctac cgactaagac aggccacgtg cgcagattga
     8101 cgcatgcctt tgccgcgtcg tatacgggtt cgggcgggtc cagctgcgag acgaacgcgc
     8161 cgcgacgcgc ctgcaagatg gtatgggtag agatgaatga atgcagcatg atagcgttgc
     8221 gcagggtcgc aaccgttccg gtttcgggcg tcctgttctc aatgcgcaca ctcagcttga
     8281 aaagtcccat atccaactgc tttgcggcga tactgattgt tccctcgatt ggatactgct
     8341 tccgcatgac catgccgggc gactcttcag caatttcgac catacgctcg cccggaatag
     8401 tgaaatcaat cgtgtaggga tgagccacca gctcgctcaa agacagattc tgcgcggcaa
     8461 tctcgcgttc gaccccctcc tcgcgcggtt cgtcggcgaa acggcgcgcc aggctccaat
     8521 caccggtatt cgatacgcca gcatgggtct gcggctgttc gacggtgcgc agaagcaggt
     8581 gcaggaatcg gacgtagaca tcgagcgagg tactcgctat gcctacgacc aggcactcgg
     8641 tctgcatctc ccagggttcg atgccgccgt tcgcctcgct gtactcctgt ggatagacga
     8701 cgccaatggt ccagcgctgg cgatttttga tggccgactg gcgataggga tagagtagat
     8761 acccctcgta cagtacagcg ttggcgattt ccttcacctg ttcgaggctc atgggtaaac
     8821 ctcctcgtcg ctggactgta acaggcgagt aatcacatct tcccacctgg gcaggctgtg
     8881 tgtggctttg tagtggtaga gctggtcaaa tacgtctttg tgtagtcgaa tccaggcgct
     8941 gttgggatag tagcgcgcga tcatctcctt ccaggttgcg accggcaaac ggtagaacga
     9001 ctccttggac caggggatct gcgcgacctg caaattgccc tcttcgccct cgtaaaagat
     9061 cgtgccgctg aacatgaacg ccagtggtat ctcgccatcc tcaagggcgt caaaatattt
     9121 ggtagaggcg acctcgaaat cataggtaca ggtgatcggc aaatccgtca caaggcttcc
     9181 atcaaactgc gggatgaccg agctggtatt tgtccataag agcggatgca gcgtatcgcg
     9241 ccatcttttg ggctcgccaa agagttcaag caggcgttcc tgcgcttcag ggctgtaacg
     9301 acggcgtgtg accgcaatct gaacctggct tctgagcgag accgagtgga tacgctcctc
     9361 ttcgtgcgcg ttcgtgacac gcagcttgaa cgtcagcgtc ggcacggcgg cataggctgg
     9421 aacctcggca tcgatgatct caaagctcag gtcaggcatg atctacccct ttcacgaaaa
     9481 ccggctgcgc ttttcgctga acgtcttgaa agtatcccgc gacggcctcc catacctctt
     9541 tgcctccgcc aagcccgcgc caggaaagcc ggatcaatcc gacgagatgg taacaggcgt
     9601 cgattggaac gatatagtag gcgcggacat cctttatacg attgatcagc agcgcttcga
     9661 catcgggcgc aagcgtattc aagactggat tgttgttgac gagcgcctgc catccctcca
     9721 ggtcgagcag cgattcggtc gccccggcag ggctgggata gtaggccatg accggctttg
     9781 ccccgccact gcgaaagatg aaggccatgt tgaccgggat catcagatcg tcccactgat
     9841 catccgccat ctgaaaatcg accagagcct gataacgcct ggggacgagc aaatacttcc
     9901 ctgcgccagc gccctgttcg ctgaacagca aggaacaggc ctggcagacg cagaccagcg
     9961 cgcgattaga gaggttcagc aggtgagaat ggctggccgg aatgccctcg ctgcacagtt
    10021 cgcagtgttc catctgcgcc tctctccgtc tctgcgcaaa gccgcgcaag gccggcatgg
    10081 aagccacgtc gacaggagca atagacgagc cattatcccc atcgttggag agatacggag
    10141 ctggcatgat cctcaccttc cattcttcaa gctcttcccc ggcacagcca gttgagcgcc
    10201 ctcacggttt cttcgagggg gaacgaatgt gatcggcaca ccacacgtga tgcccggagg
    10261 actgtccggg ttgatagcgg tagtcccatc ggcgacacca tccacatcta acctttccaa
    10321 atctggcgcc gctttgcgaa ccgctccttc aatgagttgc ttgagtgtca ccgttgagga
    10381 tggacagccg tgacagctgc cattgagacg caggtaggcg acgttgtcct cgattttgag
    10441 gaattcgaca ttgcccccat gtaatacgag ctgcgggcgt acctcgtcaa gcgcgcgcat
    10501 gacgcgcgtc tcaagatcga ccgggtgcag accgtgcaga atgagcagcg acccgatcag
    10561 gtcatcgcgc gtgaacgacc tgaccagtgg gaagccggcg gcatctgcct gaatcgaaag
    10621 ttcgagtatg cgcgccaggc attcgccgta catatccagg atgcactgga ccaactcctc
    10681 ggtcgtttca cgcgcatgaa tattagagaa cgaactgact tgctggacga gcgtctcaat
    10741 ccgctcagcg cggctttgag tttgagcatc ctgtttcatg acgctgctcc tctttcgatt
    10801 accgactaat tgccggtagg aatgcgataa cgaagattct cgatgaacta tcggacattc
    10861 attatcgcat ccccacacat caccggcaag ccatcaaaca ccgaaggtcg gcgaatgcac
    10921 gacttgcagc tctttgccat cgccaaggta catatgcaca ccgcacggca ggcaggggtc
    10981 gaagctgcgc accgcgcgca tgatatcgat gcccttgaac ttgtccgggc cgttatcttc
    11041 aaagatgggc gtattttgca cagcatcttc atacggtccc ggagtgccat agatgtcgcg
    11101 cacgctgccg ttccagggtg tgggaggata gggatggtag ttggcgatct tgccgttctc
    11161 gatgaccatg tggtgagaaa gcacgccgcg caccgcctcg tggaagccac agccgatagc
    11221 atccttcggt acggtgaatt cgttccacgt cttggtatga ccggcgtgaa tctcggccag
    11281 cgccttatcg acacagtgca gcgcgaccgc ggcggagtat gcctggaagt aggtgcgagc
    11341 gcggttgcgc tcgatggcgt tgctccactt aggaatttgc cactcgaagt ctaccgccgg
    11401 tttggtggcg gacttgggca aggtgatctt gacgctgtga ccagttgctt cgatcaaacc
    11461 cggcaaatgc acaagacccg ccagcgcggt tgtccacagg cgcgcgatcg cgccgccgcc
    11521 agtatcgagc gccaggtaat caccggtgcg cttgtcgtac cagcgtggcg acatcgtcca
    11581 gctgtacttg tcgtcgaagt tgcgcttctg cggcttcgga atcgtggtct gattccaggg
    11641 gtggcgtttg tcaaccgggt tgccaagtgg gtcgcggtcc acgaaggtgc gctcgttgtc
    11701 ccagtcgtca tagtaggaat gaccgagcag gatgcggatt cccaggttga tatcgaccag
    11761 gctggtcgtg accagttcat tatcgacaat cacgccaggc gtgacaaaca tggcgcggcc
    11821 ccactcgtcc atagtgcgat agtcatagtc gcacacatcg gggtcctgcc acgatcccca
    11881 gcagccgatt agcgtgcgcc ggtagccgac ctgttcgtag ccgggcagag cctcgtagaa
    11941 gaagtcaaac aggtcatcgt gcaggggaac catcttcttc atgaactcga cgtagcgcat
    12001 caggcgcacc aggtaatccg tgaagagctg cacggtggcc accgtgccaa cgccaccggc
    12061 atagagggtc gatggatgca catgacgccc ctccatcaag cagaatttct cgcgcgtata
    12121 gcggctgacc tgcaagccct caaggtacag ttcgccggtg aatggattga aggcgcgcat
    12181 aatgtcgcca atggtgcggt agccatgctc cctggcgtgt ggggcctccg tcttattggc
    12241 cagctccagg acacccggat tggtctcttt gaccatctgc tcgcagtagt caacggcgac
    12301 caggttctcc tggaagatgt tgtggtcgaa catgtactcc gctgcttcgc ccaggttgag
    12361 aatccattcg cccaggttgg gaggcttaac cccgtaggcc atattctgcg tgtagaccga
    12421 gcaagtggcg tggttatcgc cgcagatacc gcagatgcgg ctggtgatga agtgggaatc
    12481 gcgcgggtct ttgccttgca taaaaaggct gtatcctcgg aagatggaag aggtactgtg
    12541 acattcagcc accctccgat tcttgaagtc gatcttggtg tagataccca ggctgccaac
    12601 actgcgtgta atagggtccc atgacatctc tacaagctgt ggcggctgaa ctggagtctc
    12661 tcctcgtttc acctctgtag ccatgattgc tacctccttc taaagagtcg ctgtacgcca
    12721 atgtccgcag tgagttacca tcttcccgtg tagcctgaca tcaattccct gcctttatgc
    12781 cgccaggatg gttctttgtt ggcggtattc tgcgttaact tacgcagggg ccggatgata
    12841 ctgccataga gaccgatggc tcttgatgag aacttcgcgc cgggcggctc atccataaag
    12901 ggcataaatt tgtcggggaa gccaggcatc gtacaaccga tacagatgcc gccgacgttc
    12961 ggacagccgc cgatcccggc catccagccg cgtttcggaa cattacactg cacgaccggc
    13021 ccccagcacc ccagcttcac aaggcacttg ggagatccat actcggtagc gaaatcgccc
    13081 gtctcgtagt aggcggcccg gtcgcatccc tcgtgaacag tattgccgaa gagccaggtt
    13141 ggtcgatggg catcgtccag cggcatcatg ggcgccatac cagctgcctg gtagaggaga
    13201 tagaggaccg tctccatgaa gttatccggc tgtaccgggc agcctggcac gcagatgatg
    13261 ggtatgcctg cgccggtttt ccagttccaa ccaagatagt cgagcagacc catcgcaccc
    13321 gtcgggttac ccgacatcgc gtggatgccg ccatacgtag cgcaggtgcc tatagcgatg
    13381 accgccaggg ccttcggcga aagccgatcg atccactcat tggtagtgat gggttgaccg
    13441 gtggtgggat ctgttcccag ggcggcccaa taaccctcgc tcttgatctt ctcgttgggt
    13501 atggaacctt cgaccacgag aacgaaggga tcaagctctc cccgctcagc cgcataccac
    13561 caggtcatga aactgtcgcc gccaagttca aaggcgagga cgggattgtg cagatgcacc
    13621 ctgggtagcc ctggaatggc ccccagcaat acatcttcaa tgctcggctg agtagcggcg
    13681 gtgatcgaaa cggtatcgcc atcacagccc aatccagccg taatccagac gatgtggaca
    13741 tcctccacgc gcgatctggc tgccatactg cacctcctcg agcatttgct cgtggcttgt
    13801 tatagccgca ctaatagttc atcaccagtg agacagaaca cctctcgata aaatcccctc
    13861 cctctcgatg gattaccacc tgccatagtg ggcttgaata ctttttttca gacattcgaa
    13921 aaactggccg gtgagctttt ccaccgttcc cttgagcatg aacacgccaa ctttggccag
    13981 cgtgccgctc acatcgagcg tggcagtcca atccatgcgc gttgaatccg gcgtagccgc
    14041 gatgagtcgc atagcgcctg aaacctcaac tgtacttcca ggggcatttc ctcgtcccgt
    14101 caacatcata cgttccggct cttccatgcc cgaacgagtc acatccatca tgaacctgga
    14161 tttgatcgga ccgatagccg cgcccacgat agctttccag cgctcttcag ccagttcttc
    14221 aatgctctca aagccaggag cgcaggatgc tacctttttg gtgttgagga gatagtccca
    14281 cacttcctct cgaggcactg gcagggtctg cacaccggaa aactcaagac tcattgaaga
    14341 tctccttcat atcctggcta actagccttt ttcattacca cggaagcaag atcgaccagg
    14401 tcgttcaagg agacaggcac ccggtatttc atcaaccgat ctacggccag ccgcgccagg
    14461 ccggtcaagg tggcctcgtc cgcttttact gagctaagag cgaagcgggt gagactgtcg
    14521 aggctcttac gaacctgcga aagatcggtg gctaacaagg gggcaaactg tttcaacagc
    14581 tcattcccgc gcgttcccag gaattgttcg ttcaagaggc ggcgcatttc ctcaccgcgc
    14641 atgggggcaa agagaaggcc aacgccaacg ccaagaaata aaccagtgac caggcccttc
    14701 atgtttcaac atcctttctg catgttgacc ctcaagccag tccgttcatc tcaaattcat
    14761 gcatgagaca aagtaaagta tgcgtgagaa tagcctgaac cgttgtagcg ccggtagagg
    14821 agaatatgca accagccctt acagattcag tataggtgag tgactcggtc taaaacatct
    14881 gctaggtgac gggtatccta taggccatgt ggcctatttt tcgtaaggaa ccccactcgg
    14941 aatgccctta tatcctgttc gcgttatatg tagactgaaa caggccaagc tcaatggctc
    15001 gcacagcagc ctgcgtgcgg tctgagacac ctaatttggc gagaatatgt tctacatgga
    15061 tcttgatagt actcgcactc accgtcaagg cgcgagcaat ctcacggttc gtcagcccct
    15121 gggccaggag ttgtagcacc tcgcgttcgc gcggggacag ttggaccgca agcagttcgg
    15181 actggagaga cgtttcgccg gtcatatgtc cgaggacgcg catcacgatg tccgggtcta
    15241 gcaccgattc gccgcgcagg acgcgccgaa tagtggcgag tagctcgcgt tgtgttacat
    15301 ccttgagtac atatgccgag gctcctgctc tgagcgcctc gacaaggtaa tgtggatttt
    15361 cgtgcatcgt gacaagaatt acgctggttg atggactctc ttgcttgatg ctgcgacacg
    15421 tggccagacc atccatatct ggcatacgca catcaatcaa ggccaggtcg gggtgtaagc
    15481 ggcggcaaaa ctccagcgcc tcgcgaccat tttttgcctc cgccaacaat tccataccgt
    15541 gttgaccaat gagcatggtg cgcaaaccag ccctggcaag ctcatgatcg tcggcaatca
    15601 ccaatcgcgc tgacccgtgc ctatgttggc gttgaggact aatgctcatc gtattcgttc
    15661 ctttcagtct cggcaagatg actgcgaaac aggagaggcg tagtctcttt tataactgtg
    15721 ctatcagcat cgcactggtc ttttccattg gatgacagca cgggtacctc agctaccacc
    15781 agggtgccaa cgccagcttg actggtaacc aggagatggc cgccaagcag ttccactcgc
    15841 tcctgcatgg cgcgtatacc aacgtgttcg ccagggcgat agactttaaa caaggtgcgt
    15901 ggttcaaaac cacagcccca atcttgcacc tccaggcgca cgctcgcatc ctgaagcgcc
    15961 agcgaaagac ggacctgcgt ggtgtgtgag tgtttgcgaa cattggtgag cgcttcctgc
    16021 gcaaccccga acagcgtggt ttcaatcgtg cggggcaggc gctccagacc gagcgcctcg
    16081 tcaaaaataa tggtccagcc ctcgttgcgc tgagcctcga cgtgcaaacg cagggctgtt
    16141 gtcaaaccga aatcgtccag caccgtcggc cgcagaccgg caatcagccg ccgcgcctcc
    16201 ctgaccgaaa gctgcgccaa ttctagcgcc ctgttgagtt cctgcctggc ctgtggcgag
    16261 cgcgggcgat aatggcttgc cagagcctgc aggtgctggt gggcgctcac ggctacctgc
    16321 gccaatccat catgcagctc gtaggcaacg tgatgccgct cttcttcaag cagtttggct
    16381 cgctttcgct cgataatatt ctcaagcatc acgaggccat agatggcatc ccctctttgg
    16441 ttacgaagaa tcgtggcagt cagatcagcc caaagtgttt cctggttctt cttcagatag
    16501 cgcttttcca ctttatagct actcatctca ccagatagcg cctttccggc aagcagcatg
    16561 tccgccccga catcatcggg atgggaaagg gccgagaggt tgaagccgat cagctcttgt
    16621 tcgtcatatt ccaacatctc gcaaaaggct ttgtttacct gcagcacgtg gccttccagg
    16681 tccacgatgg ccatgccaat tggcacctcg gcaaatatgt tgcgaaagcg ttcctcgctt
    16741 tctctcatat tctcgatggc tcgttcgcgc gctttcggca cgagatccag gagacgggag
    16801 atgcatagtc ccaacaagaa cgccaagcca gagatgagaa aagcgaaaga agtaagattc
    16861 aaatacggca ttcctttcat gctcacgaac gttccgaaat cgccagtgaa tgctcaaaag
    16921 aaacggtata
//
LOCUS       DASZAK010001003         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_558037, whole genome shotgun sequence.
ACCESSION   DASZAK010001003 DASZAK010000000
VERSION     DASZAK010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558037"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VGT44_17660"
     CDS             <1..226
                     /locus_tag="VGT44_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582689.1"
                     /translation="LSGTSFYLYRNTLRYFDDIHRCLARTELILLGNSLCMQIHNLEK
                     RNEAYMELIKNVTQLTILIGEGEQKLNQQN"
     gene            complement(297..2321)
                     /locus_tag="VGT44_17665"
     CDS             complement(297..2321)
                     /locus_tag="VGT44_17665"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing
                     serine/threonine-protein kinase"
                     /protein_id="HEV2582690.1"
                     /translation="MSVVSPIFCDTCGAANRTQALFCYACGQPLSAQGGMPGTQARGT
                     TGLLTARHLLKQRYRILQQVGKGGFGAVYKASDSQFGHRLVAIKEMSQASLSQTELVE
                     ATEAFKREAMLLAGLTHPNLPRIYEQFTDMGRWYLVMDYIEGVTLEDHLGKLPGNRLP
                     IDEALEIAIQLCQVLDYLHVRQPPIIFRDLKPANVMLTRYGHCYLIDFGIARHFKPGQ
                     AKDTTALGSTGYAAPEQYGKAQTTPRADLYALGATLHQLLSGSDPAETPFQFAPLHLA
                     GQPMLANLEQLVLQMVEMDASKRPASAAAIRQELQAIATHLLIGKTNPLPAGVPPAYQ
                     PPFPGQAQSTHAPQQIVPQRTRKGQRAPKPQPQLQPNTRFICKGHTSRVTAVAWSPDS
                     AKVASASYDKTVRIWDTAHGFSVITYRGHWDRVQAVAWSPDGKRVASAGDDGTVQIWE
                     AATGSHIFTYRGHHLAVNALAWSPDGTRLASGGADKTVQVWEAANGAAVFTHRGHHGS
                     VLALAWSPDGRRVASGSADKTAQVWTAGKAKSGFFTMLLSTARNEFTYRGHSAKVQGL
                     AWSLNGLRLASASADKSLQVWDTASGKKYFNYRNGNAALNAVSWSPDNRCMASCGNDR
                     LVSIWDSVTRHSVYVYGGHTGYVTAVAWSPGGKLLASASVDHTVHVWEKP"
     gene            complement(2384..3367)
                     /locus_tag="VGT44_17670"
     CDS             complement(2384..3367)
                     /locus_tag="VGT44_17670"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEV2582691.1"
                     /translation="MQQFRPFPSHGDDTADVYISIDERQSENLLMPFPTPITQAVPAM
                     PLIFLASGLQERRTDDLVAVSSPLSPETGTLSLMAALQSTMSVETGERVVVIPAEKKR
                     VRATGQLARPRRSMSLRLRHGLVASIVFLVLVMTLLSLTPLASGQGGFQVFGNFGHWV
                     SQQAASWAIQSHSAPMTQQQPVTASSNNNAVPPPMSLPKSQYIAIAQQDATNAGISPT
                     YFVRQINQESGFNPNAVSPAGAEGIAQFEPGTAAGLGIDPWNPVQSLQAAAHLMANYS
                     SNYGGNYAMALAAYNGGPGTVTYAVNTCGASNWLNCLPYETRHYIYVIMGI"
BASE COUNT          654 a         1026 c         1047 g          722 t
ORIGIN      
        1 tctatcaggt acgagtttct atttgtatcg caacacgttg agatactttg acgatattca
       61 tcggtgcctc gccaggacag aactgattct tcttgggaat agcttgtgta tgcaaatcca
      121 taatctggaa aaaaggaacg aagcatatat ggagcttatc aaaaatgtca cgcagcttac
      181 aattctcatt ggagaaggcg aacagaaact aaatcagcaa aattgagggc gagcctggag
      241 ataaatttta tcagacctct ttttgagagg tgtatcagtc tgcttccctt atctgcttac
      301 ggcttctccc acacatgcac cgtgtgatcc acgctggctg acgcaagcag cttgccgccg
      361 ggcgaccagg cgactgccgt cacatagccg gtatggccgc cgtagacgta gacggagtgg
      421 cgcgtaacgc tgtcccagat ggataccagc ctgtcgttgc cgcacgatgc catgcagcgg
      481 ttatcgggcg accaggagac ggcgttcagc gcggcattgc cgttgcgata gttgaagtac
      541 ttcttgccac tcgcggtgtc ccagacctgc aagctcttat ccgcgctggc cgaggcgagg
      601 cgcaggccgt tgagggacca ggccagcccc tgcaccttcg cgctgtggcc gcgataggtg
      661 aattcgttgc gcgccgtcga aagcagcatg gtgaagaagc cgcttttcgc cttgccggcc
      721 gtccagacct gcgcggtctt gtcggcgctg ccggatgcca cgcgcctgcc gtcgggcgac
      781 cacgctagcg ccagcacact gccatgatgc ccgcgatgcg tgaagaccgc cgcgccattc
      841 gcagcttccc agacctgcac ggttttgtct gcccctcccg acgcaaggcg tgtcccgtcg
      901 ggcgaccacg ccagcgcgtt gacggccagg tggtggccgc gataggtgaa gatatggctg
      961 ccagtcgcgg cctcccagat ttgcaccgtg ccatcgtcgc cagccgaggc cacgcgcttg
     1021 ccatcgggcg accacgctac cgcctgcacg cggtcccagt ggccccgata ggtaatcaca
     1081 ctgaagccgt gcgcggtatc ccagatgcgc accgtcttat catagctggc cgaggccact
     1141 ttcgcgctat ccggcgacca tgccaccgcc gtcacccggc tggtgtggcc cttgcagatg
     1201 aagcgcgtat ttggttgcaa ttgcggctgc ggctttggcg ctcgctgccc tttgcgcgtg
     1261 cgttgtggga cgatttgctg cggcgcgtgc gttgactgtg cctgtcctgg gaacgggggc
     1321 tgataggcag gcggcacccc ggcgggcagc gggttggttt tgccgatcag cagatgtgtc
     1381 gcgatggcct gcagttcttg tctgattgcg gctgcgctgg cggggcgttt gctggcatcc
     1441 atctcgacca tctgcaacac gagctgttcc aggttcgcca gcataggctg cccggccagg
     1501 tgaagcggcg cgaactgaaa gggcgtttcc gccggatcgc tgcccgataa aagctggtgc
     1561 agcgtcgctc ccagggcgta gaggtcggcg cgcggcgtgg tctgtgcctt gccgtactgc
     1621 tccggcgcgg catagcccgt cgagccgagc gcggtggtgt ctttcgcctg ccccggcttg
     1681 aagtggcgcg cgatgccgaa atcgatcagg tagcaatgcc catacctggt cagcatcacg
     1741 ttagctggtt tgagatcgcg gaaaataatg ggcggctggc gtacgtgcag gtaatcaagc
     1801 acctggcaga gttggatggc gatctctagc gcttcatcga tgggcagcct gttgcccggc
     1861 agcttgccca ggtgatcttc cagcgtcacg ccctcgatat agtccatcac caggtaccag
     1921 cgccccatat cggtgaattg ttcgtagatg cgcggcaggt tgggatgcgt cagcccggcc
     1981 agcaacatgg cctcgcgttt gaaggcttcg gtcgcctcaa ccagctcagt ctggctcaga
     2041 ctggcctggc tcatttcctt gatcgccacc agccggtgac caaactggct atcactcgct
     2101 ttatagaccg cgccgaatcc acccttgccc acctgttgca aaatgcgata acgctgcttg
     2161 agcaggtgac gcgctgtgag cagccccgtt gttcccctcg cctgcgtacc cggcatccca
     2221 ccctgcgcgg aaaggggctg cccgcaagca tagcaaaaga gtgcctgcgt gcgattggcc
     2281 gcgccgcatg tatcgcaaaa gataggggaa accacggaca tacacgctgc tccatagtgc
     2341 tttcaacgtt ccggcaaaat ggtagcatag ggtagaacaa atgtcaaatt cccataatca
     2401 cgtaaatata atgtcgcgtc tcatagggta ggcagttcag ccagttcgat gctccgcagg
     2461 tattgacggc gtatgtcacg gtgccagggc cgccgttgta ggcggcgagc gccatagcgt
     2521 agttgccgcc atagttactg ctataattcg ccatcaaatg cgcggcggcc tgcagcgatt
     2581 gcacggggtt ccaggggtcg atgcccagcc cggcggctgt gcctggctca aactgcgcga
     2641 tgccttccgc gccagccggg gagaccgcgt tggggttgaa accactctcc tggttgatct
     2701 ggcgcacgaa ataggtcggt gagataccgg cattggtggc gtcctgctgt gcgatagcga
     2761 tatactggct cttgggcagg ctcattggtg ggggtacggc attattgtta cttgatgctg
     2821 tcaccggctg ctgctgggtc ataggggctg agtgggattg gatcgcccag ctcgcagcct
     2881 gctggctcac ccagtgcccg aaattgccga aaacctggaa ccccccctgg ccgcttgcca
     2941 ggggtgtcag cgaaagcagc gtcatcacca gcaccaggaa gacaatcgag gccaccagcc
     3001 catgccgcag gcgcaaactc atgctacgtc gaggacgcgc gagctgaccc gttgccctca
     3061 cccgcttctt ctccgccggg atgaccacca cgcgctcacc tgtctcaacg ctcatagtcg
     3121 attgaagcgc cgccatcagc gagagcgtac ctgtttctgg cgagagcgga ctggaaactg
     3181 caaccaggtc atcggtgcgc cgctcctgca agccgctggc aagaaagatg agtggcatgg
     3241 cgggtacggc ttgcgtaata ggagtcggaa atggcataag aaggttttcg ctctggcgct
     3301 catctatgga aatatagaca tctgccgtgt cgtctccgtg tgacgggaac ggacggaatt
     3361 gctgcattgg ctgatccttc accttcaggg acacatcggc gtgcgtgtcc atttccgcgg
     3421 ggcatccaac agtgcccctg gttgactac
//
LOCUS       DASZAK010001004         1995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_487477, whole genome shotgun sequence.
ACCESSION   DASZAK010001004 DASZAK010000000
VERSION     DASZAK010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487477"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..705)
                     /locus_tag="VGT44_17675"
     CDS             complement(49..705)
                     /locus_tag="VGT44_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2582692.1"
                     /translation="MSAPIRILIVDDQALFREGLHTLLSVQPDLQVVGEARHGEEALR
                     LAAVLTPDIILMDLHMPILDGVAATRRLQSTHPAIKVLVLTTFDDDEDVFEGLRAGAV
                     GYLLKDVPSEKLVEAIRGVARGESFLEPSLVNKIIAEFARLSPQRPSSSQHLIEPLSE
                     RELEILRLLGQGASNKEMADQLVITVGTVKNHLTSILGKLGVRDRTQAALKAKELGII
                     "
     gene            complement(702..1892)
                     /locus_tag="VGT44_17680"
     CDS             complement(702..1892)
                     /locus_tag="VGT44_17680"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV2582693.1"
                     /translation="MRLSVARKEGHLMKTRDPLAVFPQRQYRSGLFLLIVAAYAYLLV
                     TIPFDLWHVAVLVGLGLLYTVVGLFGLEYCWRSPSRPLMLVYFIALGGVLGAILFLSL
                     GEGFLLLVPLAAYSIALLPRRGAVAVCGLLLLVLGFVDWRVGDTWQSILLIVLSMLAL
                     CVFVVVFTEAVVLEAQARHALGEAHQRLREDSAHIKELATAAERNRLAREVHDNLGHY
                     LTIINVQLEAARATLDVDPTRTRSALEHAQKLTRDGLAEIRGSVAVLRALPTENRSLP
                     EALADLVRESQAADVPTTLLVPGVLPPLSSPVKFALYRIAQEGLTNVRKYAHATHVDL
                     TLDGTEGHLVRLRVQDNGVGSLDTTDGFGLLGARERARALGGQLRTASSPGAGFLLEV
                     ELPL"
BASE COUNT          411 a          615 c          637 g          332 t
ORIGIN      
        1 ctatggctgc tgctgctcga actgaagtct gagggagaag ggatcagatc aaatgatccc
       61 cagttccttg gctttgagtg ccgcctgcgt gcggtcacgc acccccaatt tgcccaggat
      121 gctcgtcagg tgattcttca ccgtgccaac ggtgatgacc agctgatcag ccatctcttt
      181 gttgctggcc ccctggccaa gcaggcgcag gatctccagt tcgcgttcgg agagcggctc
      241 tatcaagtgt tgagatgagg agggtcgttg gggggacagg cgcgcgaact cagcaatgat
      301 cttgttcacc agggaaggct ctaagaagga ctcgccacgc gcgaccccgc ggatggcctc
      361 gaccagtttt tccgagggga catctttgag cagatagccg accgctcccg cgcgcaggcc
      421 ctcaaacacg tcctcgtcgt cgtcgaaggt ggtcagcacc agcactttga tggcgggatg
      481 cgtgctctgc aggcggcggg tcgccgccac gccgtccagg atgggcatgt gcaggtccat
      541 caggatgata tccggggtga ggacagccgc caggcgcagc gcctcttccc catggcgagc
      601 ctctcccacc acctgcaggt cgggctgcac ggagagcagg gtgtgcaggc cttcgcggaa
      661 cagcgcttgg tcatcgacga tcaggatgcg aatgggagcg ctcatagcgg cagctccacc
      721 tctaagagga agcccgcgcc cggggaactg gccgtgcgca gctggccgcc cagggccctg
      781 gcacgctcac gcgccccgag cagcccgaag ccgtcggtgg tgtccagaga cccgacacca
      841 ttgtcctgca cgcgtaagcg taccaggtgc ccctcggtcc cgtcgagagt gaggtcgacg
      901 tgggtggcat gcgcgtattt gcgcacattg gtcaatcctt cctgcgcaat gcgatacaag
      961 gcgaatttca ccggggacga gagtgggggc agcacgccag gcacgaggag cgtggtgggc
     1021 acgtccgctg cctgcgactc ccgcaccaga tctgccagcg cctctggcaa ggaacggttc
     1081 tccgtgggca aggcgcgcag cacggccacg gacccccgaa tttcggccaa tccgtcccgc
     1141 gtgagcttct gcgcgtgctc aagggcgctg cgggtacgcg tgggatcgac gtccagcgtg
     1201 gcccgtgccg cctcaagctg gacattgatg atggtgagat aatgccccag gttatcatgg
     1261 acctcgcgcg ccaggcgatt gcgttcggcg gccgtcgcca gctctttgat gtgcgcggaa
     1321 tcctcgcgca aacgctggtg cgcttcgccg agtgcgtggc gcgcctgtgc ttcgagcacc
     1381 accgcttccg taaagacgac cacgaagaca cacagagcca gcatcgagag cacgatcagg
     1441 aggatggact gccaggtgtc accaacccgc cagtccacga agccgagcac tagcagcagc
     1501 agcccgcaca cggcgaccgc accgcgccgg gggaggaggg cgatactgta ggcggcgagc
     1561 ggcacgagca ggagaaagcc ctctcccagg ctgaggaaga gaatcgcgcc gaggacgccg
     1621 ccgagggcaa taaaatacac cagcatgagc ggcctggacg gtgagcgcca gcagtactcc
     1681 aggccaaaca gcccgaccac agtatacagg aggcccagtc cgaccaggac agctacatgc
     1741 cataggtcaa atggtatggt gacgaggagg taggcatagg cggcaacgat cagcaggaac
     1801 aagccgctac gatactgtcg ctgaggaaaa accgccaacg ggtctcgcgt cttcatcaga
     1861 tgcccttcct tcctggctac tgagagtctc atcgtgtgaa agccagatga gtgagacgcc
     1921 ttgccttcgg acagggcgca agcaagaaaa cgtcagaatt acaccgatag gcctacagaa
     1981 cgattttctt cctta
//
LOCUS       DASZAK010001005         4300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_524668, whole genome shotgun sequence.
ACCESSION   DASZAK010001005 DASZAK010000000
VERSION     DASZAK010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524668"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VGT44_17685"
     CDS             <1..273
                     /locus_tag="VGT44_17685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2582694.1"
                     /translation="SYELGDGWVNTLAVLRPWRKQGLGMALLLHSFREFYQRGKRKVG
                     LGVDSQNLTGATRLYQRAGMHVAREHITYEKELRAGIELSTQILPA"
     gene            327..1010
                     /locus_tag="VGT44_17690"
     CDS             327..1010
                     /locus_tag="VGT44_17690"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2582695.1"
                     /translation="MSVLLANLVARTPTSEDFYAIAELVVACDIAEHGIADSSTRDLA
                     ARWQNYNFHLDSDAWVIVNSRKQFVGFACVWHRDHEEFYTFICVHPQYRKRGIGTLLL
                     RMAEQHARELMRHACEGARVSLRGVVSAGNAQARSLFEREGYLATREFWRVTMELLES
                     NGDGAGQAGTFSIDLDIESGLLIGAAPLYDREGVYSLRQHVTYEKELRPASEPCDCPD
                     EDAETLVEA"
     gene            1301..2629
                     /locus_tag="VGT44_17695"
     CDS             1301..2629
                     /locus_tag="VGT44_17695"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582696.1"
                     /translation="MRSFPWNNRIEKQSKFARLARSSVIPLLLGTFILRTNTGAGSIV
                     LGRFLARLSAAHGHPISSINVGLISVAYYIIELSLAPLLGAFSDRLGRRLFLVVGPFF
                     GLTQVALLIFTPVANPFPYLIALQLLAGISSAMQVPAALGYLADFTAREHSLRMRIMS
                     MYELATSGGIAVGVVIGGFAWDRFGRYAFVLLAMNYLLVALCMSLVPAVRQVIERGKM
                     RTLARRFWRVIRTPRLFIFIPAWISINALVGVWLSSQLTFVLSEPRHEPHQFLVGSLS
                     AAGSGGRLSLILGLYALFFGLCLLFWAFFLNRAPRLLLMLASITGVYLACIALFGLNH
                     SGFDLTPLLVLWVALLSLGVFAESSFAPAALAYLADISEEVAKDRGLLMGLYAVFLGL
                     GQLLGNGLGGVFAQVWGFDGLVYLTAILAFIALVCLVSLSQLERRNASAE"
     gene            2659..4236
                     /locus_tag="VGT44_17700"
     CDS             2659..4236
                     /locus_tag="VGT44_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HEV2582697.1"
                     /translation="MTKHAKITIIIALMLGMSLAALDTTIVGTALPTIVGKLGGITLY
                     SWVFSAYLLTSTTTVPIYGKLADLFGRKPIFLFGSGLFLLGSLASGASQTMVQLIIFR
                     AVQGLGAGAVLPIVLTIIGDIFSLEERARVQGLFSGVWGLTSIIGPALGGLIVDHFSW
                     RWVFYINLPFGLLSMVLLTIALKENVARQKRSIDYTGSLTLTAGIVALLFALLQGGQS
                     WAWLSWQSLGLFAMSIVFLALFFYQENRAQEPILPLTLFNNRIISISSIGAVILGVVM
                     FGVTTYVPLFVQGVGGGTATSAGLTLGPLLLAWPIASTLSGKIIIRYGYRFTAVAGSL
                     LVTLGVGMVTFYNVGTTLPFVVVSMLVIGAGLGLMSMAFLLSVQNAVPWNVRGVATAS
                     NQFFRSMGGTVGVAIMGTILNVQMAALFTPIFASHASVVASLPKGLAPSNVLLTPSVR
                     AALPADFLGQLVNALSHSLFWLYLLMLVLALIGLAAMFLLPGGRAEQYAYHAEKGEES
                     VEQEAEGAGEVEITTFG"
BASE COUNT          720 a         1278 c         1224 g         1078 t
ORIGIN      
        1 tcctacgagc ttggcgacgg ctgggtgaat acgctggctg tgctgcgccc ctggcgcaaa
       61 cagggacttg gtatggcgct gctgcttcat tccttccgcg agttctatca gcgcggcaaa
      121 cgcaaggtcg ggctgggtgt cgattcgcag aaccttacag gggcgacgcg cctctaccag
      181 cgcgcgggta tgcatgtcgc gcgcgagcac atcacttatg agaaggagct gcgtgccggt
      241 atcgaactga gtacgcagat cctcccggct taggggagtt cttcccactc cttctcgcag
      301 ttggctttag aacggagata ctggcaatgt cagttttact agcaaacctt gtggcacgaa
      361 caccaacttc tgaggacttc tacgcgattg cggaactggt agtggcctgt gacattgctg
      421 agcatggcat cgcggatagt tcaacgcgag acctggctgc tcgctggcag aattataact
      481 ttcacctgga tagcgatgcc tgggtcatcg tcaacagtcg aaagcagttc gtcggcttcg
      541 cctgcgtctg gcaccgggat cacgaggagt tctatacatt catctgcgtg catccgcagt
      601 accgcaaacg cggtatcggt acgctgctgc tgcgcatggc ggagcaacac gcacgcgagc
      661 tgatgcgcca tgcctgcgag ggcgcgcgtg ttagcctgcg aggtgtcgtc agcgcgggca
      721 acgcccaggc gcgcagcctg ttcgagcgtg agggctacct cgctacgcgc gagttctggc
      781 gcgtcacaat ggaactgctc gaatcgaacg gcgatggcgc cggtcaagcg ggtacgttct
      841 cgatcgatct agatatcgag tcaggtctgc tgatcggagc cgctccactc tatgaccgcg
      901 aaggcgtcta tagcctccgc cagcacgtca cctatgagaa ggagctgcgc cccgccagcg
      961 agccgtgcga ctgccccgac gaagacgctg aaacactcgt tgaggcttga gaatgaatac
     1021 ccagggcaac gttattgttg ccctgggtat tcagtacccc tagttacttt tgatttgaat
     1081 acgccgttga agatcatgtg agggaagtcc tgtggccatg ttactattgc gcatggagaa
     1141 tcatctatgt atcttctggc aacggtcgta tttggaacga tagacactgg gggaatgcgt
     1201 ccgctctact tgcttatgtt gctgcttgcg attatcctgg tactaacggg gtgtgtgatt
     1261 gcactgctaa cattgcggaa ccaacgaaag aacggtcagt ttgcgctcat ttccctggaa
     1321 caaccggatc gagaaacaat ctaagttcgc gcggctggcg cgcagttcgg tgatcccgct
     1381 gctgcttggc acgtttatcc tgcgtaccaa taccggcgcc ggttcaattg tgttaggacg
     1441 ttttctcgcc cggctttctg cggcgcatgg ccatccgatt tctagtatca atgtcggttt
     1501 gatctcggtg gcctattaca tcatcgaact ttcgcttgct cccctgcttg gcgcgtttag
     1561 cgaccgcctg gggcggcgcc ttttcctcgt agttggtccc ttctttggtc tgacccaggt
     1621 tgccctgctg atctttacac cggtagcgaa cccctttccc tatttgatcg cattgcaact
     1681 gttggcgggc atctcttcag ccatgcaggt accggcggcg cttggctacc tggccgattt
     1741 tacggctcga gagcactccc tgcgtatgcg tatcatgagc atgtacgaac tggccacaag
     1801 cggtggcatc gccgtcggcg tcgtgatcgg cggtttcgcc tgggaccgtt ttggccggta
     1861 cgcctttgtt ttactggcca tgaactacct gctggtcgcg ctctgtatgt ccctggtgcc
     1921 tgcggttcgg caggtgatcg aacgcggcaa gatgagaaca ttggcccgcc ggttctggcg
     1981 cgtgattcgc acaccgcgcc tgttcatttt cattcccgcc tggatcagta tcaacgcgct
     2041 cgtcggtgtc tggctcagct cgcagcttac cttcgtgcta tccgagccgc gtcacgagcc
     2101 gcatcagttc ctcgtgggca gccttagcgc ggctggtagc ggcgggcgat tgagcctgat
     2161 tctgggcctt tacgccctct tttttggcct gtgcctgctc ttctgggcct tctttctgaa
     2221 ccgcgcccca cgcctgctgc tcatgctggc ttccattacc ggcgtctatc tcgcctgtat
     2281 tgccctgttc ggtctgaacc atagtggctt cgatctcaca ccactgctcg ttctctgggt
     2341 tgcgctgctc tccctcggcg tcttcgcaga aagtagcttc gcgcctgctg ccctggccta
     2401 tctcgccgat atctcggaag aggtcgcgaa agatcgtgga ctgctgatgg gactctatgc
     2461 cgtctttctg ggtctaggcc aactgctcgg caatggcctg ggaggtgtct ttgcccaggt
     2521 ctggggtttc gatggcctgg tctacctgac tgccatactg gccttcatcg ctctagtctg
     2581 cctcgtctct ctctctcaac tcgaacggcg taacgcctcg gcggaataat ctgagcggcg
     2641 gttgtgttgt attgtaacat gacaaaacac gcaaagatta ccattatcat tgctttgatg
     2701 ctgggcatgt cgctggctgc gctcgacacc acgattgttg gcacagcgct gccaaccatc
     2761 gtcggcaaac tgggcggcat caccctctat tcatgggtct tttcagccta tttgttgact
     2821 tcgacgacca cggtgccgat ctacggcaaa ctcgccgatc tcttcgggcg caagccgatc
     2881 tttctctttg gctccggcct ctttctgctc ggctctctgg cgtcgggcgc gtcccagaca
     2941 atggtgcagc tcatcatctt ccgcgccgtg cagggcctgg gtgcgggcgc ggtcctgcca
     3001 atagtgctga cgatcatcgg cgatatcttt tcgctggaag aacgcgcccg cgtgcagggt
     3061 ttgtttagcg gcgtatgggg cctcaccagc attatcggcc cggcgcttgg cggcttgatc
     3121 gtcgatcatt tctcatggcg ttgggtcttt tacatcaatc ttccctttgg cctgctctcg
     3181 atggtcttgc tcactatcgc gctcaaagaa aacgttgcac gccagaaacg ctctattgac
     3241 tataccggtt cactcacact gaccgccggc atcgtggcgc tgctctttgc cctgctccag
     3301 ggcggccaga gctgggcctg gctgtcgtgg cagagccttg gcttgttcgc catgtcgata
     3361 gtcttcctgg cgctcttctt ctaccaggaa aaccgtgcgc aagaacccat tctgcccctg
     3421 acgctcttca acaaccgtat catctccatc tcaagcatcg gagcggttat tctgggtgtg
     3481 gttatgttcg gcgtcaccac ctacgtcccg ctttttgtgc agggcgtcgg cggcggtacg
     3541 gcgaccagcg ctggtctcac gcttggcccg ctgctgctgg cctggccaat tgcctcgacc
     3601 ttgagcggta aaatcatcat tcgttacggc tatcgtttta ccgctgtcgc gggttcgctg
     3661 ctcgtcacgc tgggcgtcgg catggtgaca ttctacaatg ttgggacgac gctgcccttc
     3721 gtcgtggtgt caatgctggt gatcggcgcc ggacttggct tgatgagcat ggctttcctg
     3781 ctgtcggtac agaacgccgt tccctggaac gtgcgcggtg tggcgaccgc ctcaaaccag
     3841 ttctttcgct cgatgggcgg taccgtcggt gtcgctatca tgggaaccat cctgaatgtg
     3901 caaatggccg cgctcttcac gcccatcttc gccagccatg cctcggtcgt ggccagcctg
     3961 ccgaagggtc ttgcgccctc caacgtgctg ctcaccccgt ccgtgcgcgc cgccctcccg
     4021 gccgatttcc tcggccagct cgtcaacgcc ctatctcaca gcctcttctg gctctatctc
     4081 ctgatgctgg tgctggccct catcggcctg gcagccatgt tcctcctgcc cggcggtcgc
     4141 gccgaacagt atgcgtatca tgccgagaag ggtgaggaaa gcgtggagca agaggcggag
     4201 ggtgcgggcg aagtggagat aacgacgttt gggtagacct cccaaaaata gtcccatttc
     4261 gcttgaatag cgccgcaaca tatggtatgc tgccagcata
//
LOCUS       DASZAK010001006         9384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_666234, whole genome shotgun sequence.
ACCESSION   DASZAK010001006 DASZAK010000000
VERSION     DASZAK010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9384
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666234"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..915)
                     /locus_tag="VGT44_17705"
     CDS             complement(118..915)
                     /locus_tag="VGT44_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2582698.1"
                     /translation="MSTQDILPLEGKRAIVTGAGRGIGRSIAIALARAGADVGITART
                     FEELEEVGMEINGTFGRHCASILCDVTDPAQVEEMASALINSLEGVDILVNNAGNAGS
                     HKFLNHPDELWHRMIAINLNSVYYVTKAIAPAMVKQRSGRIITIASIASRVGGSYIAA
                     YTAAKHGVLGLTRALATELMQYNITVNAICPGYVDTPMTDGSVANIAARTGMSAELAR
                     ETLTRTSPQRRLIEPEEVAAIAVFLAQESSKGITGQAINIDGGGVMS"
     gene            complement(930..3110)
                     /locus_tag="VGT44_17710"
     CDS             complement(930..3110)
                     /locus_tag="VGT44_17710"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV2582699.1"
                     /translation="MKIAILGGGPAGLYSGLLIKKNNPAHDITVFERNAADVTYGWGV
                     VFSDRTLASFQRADYKSYEQITNQFVIWNDIDIFYRDQTIRCGGHVISGIARKTLLHI
                     LQQRCQELGITLHFNQDINDLTPLEAFDLVIAADGVNSIARKTYAEHFQPSIAQGKAR
                     YIWLGADKVLDAFTFIFCENEHSLFQVHAYPFGGTNSTYLVECDEASWLNAGLDRADE
                     AQSLAFCQRLLDEYLNGAQLLSNNSKWISFPTLKTKNWHYRNVVLLGDAAHTAHFSIG
                     SGTKLAMEDAIALAGALEQHRVLESALNAYELERKPVVEVFQRAAAESQAYFETIKRY
                     LGLEPMQFAFHLLTRSGRISYDDLRFRDVRFGEAFDRWHAEQARVIVQGHTGGENIGP
                     VSPLYAPAPMFTPFRLRSLILPNRLAITTNPYMIDGRSTAHDAAPDAASRERILALAQ
                     RDAGLLFTGSVAVSPDGRITPQDVGIYSRESLVSWGYLVEQVHARSNAYIAIELNHAG
                     RRGATRPRSDGVDRPLRAGAWPLISASALPYSPRSQVPRAMEQADIERVRHDFIYATR
                     MARDAGFDLLQLNIAHGYLLAGFLSPLTNVRDDEYGGTLENRSRFPLEVFDAVRATWP
                     GPRPLSVALTCSDCVKGGFTIEEAVKVATMLKAHGCDMIEARVGQATSASEPAYGRGF
                     LTSYSERIRNEAGIATIVGGYLTTSNEVNTIVAAGRADLCIITV"
     gene            complement(3107..3982)
                     /locus_tag="VGT44_17715"
     CDS             complement(3107..3982)
                     /locus_tag="VGT44_17715"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2582700.1"
                     /translation="MRVGVGLPSGIPGASHELVLDWARRADAGPFSSLGVVDRLVYDS
                     FDPLITLAAVAGATSRITLATTIVTGPLHNDTLLAKMAVSLHALSGERFVLGLAVGAR
                     REDYEVAGIDYRDRGNRLTEQLLTLREAWEEGVICPRTANGRGPALLIGGLSDAGYAR
                     LSRYADGYVHGGGPPRSFARAADKARAAWQDAGRPGQPALWAQGYFTLGSEAECEAGR
                     RYMRSYYGFLGPFAERIASELLTTPQAIAQFMRGYAEAGCDELVLFPTLASNEQLDRL
                     ATALDRLGVTARSAV"
     gene            4220..5380
                     /locus_tag="VGT44_17720"
     CDS             4220..5380
                     /locus_tag="VGT44_17720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2582701.1"
                     /translation="MHTTIQPDTLTKLARMGEATAFEPSGDQPMMERRRAPFQSHSLE
                     ECITQVATPTGKKPILKTMLTTACERNCFYCPFRAGRSKTKRLTFTPDEVASALDTLS
                     GERRVDGIFLSSGIIKGSVTTQDKIIDTVEIIRNRYHYNGYVHLKIMPGIELDQLYRA
                     MQLADRVSVNLEAPTQERLSTLAPKKDFTNELLKMLRWAEMLRRNNPQEKLARTVTQF
                     VVGAVGDTDSELLSMSERLYRHAGLTRAYYSAFHPIEQTPFENLAPTDALREFRLYQA
                     SFLLRDYGWSIKDMAFLDSGNLPINIDPKHAWAERHLREQPLEIMLAERDQLMRVPGI
                     GPVGADAIIQARKRGRITELSHVRKLGIRAPERSAPYLLLDGHKALMQLHFL"
     gene            5846..9304
                     /locus_tag="VGT44_17725"
     CDS             5846..9304
                     /locus_tag="VGT44_17725"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2582702.1"
                     /translation="MELTLTRQNETQVQVACDGQLSHSFDLYSLVPTKAAQLGLPQPL
                     SDPMTYGKAAYQALFPVGSPARRILDAIPERILLITTDDDIDAIAWEYAYGPDGFLVA
                     DYPFTRGLPPDQCIVPPVLDGGLHIVAIPSDPLDKRLNPLNIDGEWARLQEIIRKVPG
                     AVRLERTYPPTIEQVRRQVANQRHRVVHFMGHGGQDDDAGAFLCLEKENGTLDPVTAK
                     RFVQRVRSSVFLVTLNACVSATPGPTQFSNLAAALAQQKVPYALGMRMNIYDDDARAF
                     SRQFYSELVSGVSVEEALLQARLTLANSSCTWAIGVPVLYTSLSEPAGGYAAQAGTPT
                     IAEHWPPIDAFALPRAEGGFQGRIDELAQLGSVLTGDDRPPVVTIHGSGGQGKTALAR
                     EAVERFAFAWPGGIWAISLENLPSREVFLTTLARFLGLPVQEISRLDDLERQVLLRLG
                     QGQPRTLLVLDNVETLIEAAHANDIAAINLAQFIRARLTRPPVSLLVTSRRYLDWQGE
                     LGLELEGLQPDEGAKLFRQSAPRRSHDIEPELAEELSRKVAGHPLSLRLLGGAFNQIT
                     LSLPEFIHDCDTHLLAAEDKYIDLGHRQRTLYASIETSVRYLDTALSQLFSRLWVFHA
                     PFLPQSAVAIYDPDFDNTKGGRSPIYDQLHALWLRGLLTLQRVTLREGTWEFYSLLPT
                     MRPYIENYLARAEEREELLARFGAAYARLARFLYNELNRGGTAAFIAGQSHEDLERGA
                     SSVTGAEQGHYFLDWGWVLQRTGYTRRGLHLTEQALEIGQGQDRSLELHALNNMAGVY
                     NATGQPKRALELYEQALPLMREVGDRAGEAATLNNMGWVYRTTGQPKRALELYEQALP
                     LMREVGDQVGEAASLNGLASLLTDMREYEDARAFIVESIRLERQVNHPAGEVAGLVGL
                     AWLLDQHLNRRQDAINAMQQAIAVLDETGLPQDAAGQTRDMLQQYLDAMRQAVALGQA
                     DQAVTMPAAQLEVIVHNTVAVMTTAQERRAEWHTTLTDALQYVQQQGAKRQNEVDFFA
                     AVLALLDGQTSSLPGDHPYAAGLAQIQAGIAAESSTNFFEMMVMVSETLAVLGPVPEQ
                     LPEWRAALLQGKEQAMQADERELVALLDAIVGLLDAGGNPAGLGADLAGDYAQMWQAL
                     VEALGK"
BASE COUNT         1993 a         2713 c         2753 g         1925 t
ORIGIN      
        1 ctgtgcatag acctcgaagg tcagcgcgtt catcacctgc gggcgattga gcgtgatggt
       61 cgcgacgcca tctgccacct cgtagaggaa atcgtagtcc atatccaaca ctccttctca
      121 ggacatcaca ccgccgccat cgatattgat ggcctggccg gtgatacctt tgctgctctc
      181 ctgcgccagg aagacggcga tagcggccac ttcctccggc tcgatcagcc ggcgctgggg
      241 gctggtcctg gtcagcgtct cgcgcgccag ttccgcgctc atgccggtgc gagcggcgat
      301 gttggcgacg ctgccgtcgg tcatgggcgt atcgacataa ccggggcaga tagcattgac
      361 tgttatgttg tattgcatca actcggtagc cagggcgcgc gtcaggccca gcacgccatg
      421 tttggcggcg gtataggcgg cgatgtagct gccgccgacg cgcgaggcga tagaggcaat
      481 cgtgatgatg cggccagagc gctgcttgac catggcggga gcgatcgctt ttgtgacgta
      541 atagacgctg ttgaggttga tcgcgatcat gcgatgccac aactcgtccg ggtggttcag
      601 gaacttatga ctcccggcgt tcccggcgtt attcaccagg atatcgacgc cctccaggct
      661 gttgatcaag gctgaagcca tctcttccac ctgtgcagga tcggtcacat cgcagaggat
      721 cgaggcgcaa tgccggccaa atgtgccatt tatctccatg cctacctctt caagctcttc
      781 aaatgtgcgc gccgtgatac ctacatctgc cccggccctg gccagcgcaa tcgcgatgga
      841 acgaccgatg ccccggccag cgcccgtcac gatggcgcgt ttgccctcca ggggcagaat
      901 atcctgagtt gacatgtgct actcctccat tacacagtga tgatacacag atcggcgcgg
      961 ccagccgcga cgatggtgtt gacttcgttg ctcgtcgtca ggtatcctcc gacgatggta
     1021 gcaataccgg cctcgttgcg gatacgttcg ctgtacgagg tcagaaaacc acgcccatag
     1081 gccggttcgc ttgcggatgt agcctggcca acgcgcgcct caatcatatc gcatccatgc
     1141 gcttttagca tagtcgccac cttcacggct tcttcgatgg taaacccacc cttgacgcaa
     1201 tcactgcatg tcaacgctac cgagagcggc cttggtcccg gccaggtcgc gcgcacggcg
     1261 tcaaatactt ccagcgggaa gcgcgagcgg ttttccaatg ttccaccgta ctcatcgtcg
     1321 cgcacgttag tgagcggcga aaggaagccc gccagcaggt agccatgcgc tatattcaat
     1381 tgcagcaggt cgaaacccgc gtcacgcgcc atgcgggtcg cgtagatgaa gtcatggcga
     1441 acccgctcta tatctgcttg ttccatcgcc ctgggaacct ggctgcgcgg cgaatatggc
     1501 agggctgaag cggagatcag tggccacgcg cctgcgcgca gcggcctgtc cacgccatcc
     1561 gaacgtgggc gagtcgctcc ccgccgaccg gcgtggttta gttcgatggc tatgtacgcg
     1621 ttggagcgtg cgtgaacctg ctccacaaga tatccccagg agacaagaga ctcgcgacta
     1681 tagataccca catcctgcgg ggtaatgcga ccatccggcg agacggctac cgacccggtg
     1741 aacagtagcc cggcgtcacg ttgggcaagc gcgaggatac gctcgcgaga tgctgcatcc
     1801 ggcgctgcgt catgcgcggt agatcgacca tcgatcatgt atgggttggt ggttatggcg
     1861 aggcgatttg gcaatatgag tgagcgtagg cggaacggcg tgaacatggg cgcaggagcg
     1921 tagagcgggc tgaccgggcc aatattctca ccgccggtat gtccctgaac gatcacacgc
     1981 gcctgttcag cgtgccagcg atcaaacgcc tcgccgaagc gcacatcgcg aaagcgcagg
     2041 tcgtcgtagc taatccgacc gctgcgtgtg agcaggtgga aggcgaattg catcggctcc
     2101 aggccaagat agcgtttgat cgtttcaaag tacgcctggc tttcggcggc ggctcgctgg
     2161 aagacctcga cgaccggctt gcgctcgagt tcataggcat tgagcgcgct ttccaggaca
     2221 cgatgttgtt cgagcgcgcc cgccagggcg atggcgtctt ccatcgctaa cttggttcct
     2281 gatcctatcg agaagtgggc ggtgtgcgcc gcgtcgccca gcagcactac gttgcgataa
     2341 tgccagtttt tcgtcttgag cgtggggaag ctgatccatt tcgagttatt cgagagcagt
     2401 tgcgctccgt tcaggtactc atcgagcaag cgctggcaaa aggcgaggct ctgcgcctcg
     2461 tccgcgcgat ctaacccggc attgagccag gaggcttcgt cgcattcaac aaggtaggtg
     2521 ctgttcgtgc cgccaaaggg gtaggcatgt acctgaaaga ggctgtgttc gttttcgcag
     2581 aagatgaagg tgaaggcgtc cagcacttta tcagcgccca accagatata cctggctttg
     2641 ccctgcgcaa tcgaaggctg gaaatgctcg gcataggttt tgcgcgcgat gctgttgacg
     2701 ccgtcggcag caattaccag gtcaaatgcc tctaaagggg taagatcatt gatatcctgg
     2761 ttgaaatgca gggtgatgcc cagttcctgg cagcgttgtt gcaggatgtg gagcagcgtc
     2821 ttgcgtgcaa tgccggaaat gacatgcccc ccgcagcgaa tggtttggtc gcgatagaag
     2881 atgtcgatat cgttccagat cacaaattgg ttggtgatct gctcgtagga tttgtaatcg
     2941 gcgcgctgaa aggaggccag cgtgcgatct gaaaagacga cgccccaacc gtaggtcaca
     3001 tcggcggcgt tgcgttcgaa gacggtgatg tcgtgcgccg ggttattttt tttgatgagc
     3061 aggccactgt atagtcctgc cgggccgccg ccgagaatgg ctattttcat acggcgctcc
     3121 tggcggtcac tcccagcctg tccagtgcag ttgccagccg gtctagctgc tcgttgctgg
     3181 ctaacgtagg aaacagcacc agttcatcgc acccggcctc cgcatagcct cgcatgaatt
     3241 gcgcgatggc ctgcggcgtg gtcagcaatt cggatgcgat gcgttcggca aaggggccga
     3301 ggaagccgta atagctgcgc atgtagcggc ggcctgcctc acattctgct tcgctgccga
     3361 gtgtgaaata tccttgcgcc cacaaagccg gctggccggg gcggcccgca tcttgccagg
     3421 ccgcgcgggc tttatctgcc gcgcgcgcga acgagcgcgg cggccctccg ccatgcacat
     3481 agccgtcggc atagcgcgac aggcgcgcat aaccggcgtc gcttaatcct ccgattagca
     3541 gcgccgggcc gcgtccattt gcagtgcgag ggcaaatgac gccttcttcc cacgcctccc
     3601 gcaaggtaag cagttgttcg gtcagccgat tcccccggtc gcggtagtcg atgcccgcaa
     3661 cttcgtagtc ttccctgcgc gcgccgaccg ccagccctag cacgaaccgt tcaccggaga
     3721 gcgcatggag cgatacagcc attttcgcca gtagcgtgtc gttgtgcagc gggccggtaa
     3781 cgatcgtggt cgccagtgtg atgcgtgagg tagcgcctgc cacagccgca agcgtgatca
     3841 atggatcgaa gctgtcgtaa acgaggcgat caacaacgcc caggctagaa aacggacccg
     3901 cgtccgccct gcgcgcccag tccaacacca gctcgtgact cgcgccagga atacccgatg
     3961 gcaggccaac tccgactcgc ataaccgcaa ccccttcgtt gctttcgcgc gctatgagga
     4021 cgctcttttt ttgttgtatt tagtatggca catagaagag aacgtctcaa gagaaatgtg
     4081 ggataacggg tggatatcct gtgctagaat aatgtttaaa gaaggtgcca tatttgatac
     4141 cgctcggcga tgagatgatg actctgagca gcagtgacga agattgacag atggaacata
     4201 cgttcagtat aatgaatgta tgcacacgac gatccagccc gacaccctga caaaactcgc
     4261 ccgtatgggc gaggcaactg cttttgagcc atctggcgac cagccgatga tggagcgtcg
     4321 gcgcgcgcca ttccaatccc atagccttga ggagtgtatc acgcaggtag ctacgccgac
     4381 gggcaagaag ccgatcctca agacgatgtt gacgactgcc tgtgagcgta attgctttta
     4441 ttgccccttt cgcgcaggcc gcagcaaaac gaaacgtctg accttcaccc ccgacgaggt
     4501 cgcaagcgcg ctggataccc tctcagggga gaggcgcgtc gatggtattt tcctctcctc
     4561 gggaatcatc aaggggagtg tgacgactca ggataagatc atcgatacgg ttgagatcat
     4621 tcgtaatcgt tatcattata atggctatgt ccacctgaaa attatgcccg gcatcgagct
     4681 ggatcaactc taccgcgcga tgcaactggc cgaccgcgtc tcggtcaacc tggaagcgcc
     4741 cactcaggag cggctctcaa cgcttgctcc aaagaaagat ttcacgaacg agttgctaaa
     4801 gatgctgcgc tgggcagaga tgctcagacg caacaatccg caggaaaaac tggcgcgcac
     4861 cgtaacacaa ttcgtcgtcg gagcggttgg cgatacggat tccgaattac tctcgatgag
     4921 cgagcggctc tatcgtcacg ctggcctgac gcgcgcctac tactctgcct tccacccgat
     4981 tgagcagacc cctttcgaga acctggcccc aaccgatgct ctacgagaat tccgtctcta
     5041 ccaggccagc ttcttgctgc gcgactatgg ctggagcatc aaagacatgg catttctcga
     5101 tagcggcaat ttaccgatca acattgaccc caaacatgcc tgggctgaac ggcacttacg
     5161 cgagcagccg ttggagatca tgctggcgga gcgagatcag ttgatgcgag taccaggcat
     5221 cggcccggtt ggagcagatg cgatcatcca ggccaggaaa agaggtcgca ttaccgaact
     5281 gtcccatgtg cgaaaactag gaatacgcgc ccctgaacga tcagcgccat atctgttgct
     5341 tgatggtcat aaggcgctaa tgcagttaca tttcttatag ccattaacta ctctcgaaca
     5401 tgagcaagtt gtactggtct tgcatcgaga caaagcgtgt ccagccatgg agatcagcca
     5461 catactgagg tagggctagg atcactgcaa aaatctatca taagcccata ggatcgctct
     5521 acaagccaat ctatgcaatg agagcaagag taacctttcg ctcgatgtat gcaggtacaa
     5581 ataaacgata gagccgcttt tcagatagat tgcgacatct tttgaagagc cttatgaacc
     5641 cgatacgaga aaattcatgc tctctcggag atttttgcag tgatcttagc cctacctcca
     5701 caaatgcagc aagagcattg accagaagtc atgaacttgc tatactctgc aaaaagagca
     5761 gagatgccct taatggattg aaaactgcta cagagcatcg ccctctctca tagagccatg
     5821 tgttgatggc atggaggcag gaaacatgga actcaccctt acgcggcaaa atgagacgca
     5881 ggtgcaggtg gcctgcgacg gccagctctc ccacagcttt gatctctact cgctcgttcc
     5941 caccaaagca gcccagctgg gactgcctca gcctctttct gatccgatga cctatggcaa
     6001 agccgcgtac caggcgctct ttcccgtcgg ttcacctgct cgccgcatcc tcgatgccat
     6061 ccccgaacgt attctgctca ttaccaccga cgatgacata gatgccattg cctgggaata
     6121 tgcttatggc cccgatggct tcctcgttgc ggactacccg ttcacacgcg gactcccacc
     6181 cgaccagtgc attgttccgc ctgtgctgga cggcggcctg cacatcgtcg ccattccatc
     6241 cgatccgctg gacaaacgac tgaaccccct caacatcgat ggcgaatggg caagattgca
     6301 agagatcatc cgcaaagtcc ctggtgccgt gagactagaa cgaacctacc cgccaactat
     6361 tgagcaggtg cgcaggcagg tggcgaacca gcgtcatcgt gtggtgcatt tcatgggtca
     6421 cggcgggcaa gacgacgatg cgggcgcgtt tctctgcctg gagaaggaaa acggcaccct
     6481 tgatcccgtc accgcgaaac ggtttgtgca gcgcgtgcgc agcagtgtct tcctggtcac
     6541 actcaatgcc tgcgtcagcg ccacgcccgg cccaacgcag ttcagcaacc tagcggcagc
     6601 gctcgcacag cagaaagttc cctatgctct gggtatgcgc atgaacattt atgacgacga
     6661 cgcccgcgct ttctcgcgcc agttctatag cgaactggta agcggtgtgt ctgtagaaga
     6721 ggccttgctc caggcgcgcc tgacccttgc caatagctct tgcacatggg ccatcggcgt
     6781 gccagtgctc tacacatcac tctcagaacc agcaggagga tacgcggctc aagcgggtac
     6841 accaaccatc gcagagcact ggccacccat tgatgccttt gcgctgccgc gcgcagaagg
     6901 cggattccag ggacgcatcg atgaactggc gcagcttggc agtgtcctca caggtgatga
     6961 tcgccccccg gtcgtcacca ttcacggcag cggcggacag ggcaagacgg cgctggcgcg
     7021 tgaagccgtc gaacgcttcg cctttgcctg gcctggcggc atctgggcca tctcgttgga
     7081 gaatctgcca tcgcgcgaag tctttctgac aaccctggcg cgcttcctgg gcctcccagt
     7141 gcaggagatc agccggctag acgacctcga gcgccaggtg ctgctgcgcc tgggccaggg
     7201 ccagccgcgc acgctcctcg tgctggataa tgtcgaaaca ctgatcgaag cagcccacgc
     7261 caacgatatt gccgccatca acctggcgca gttcatccgc gcacgtctga ccaggccgcc
     7321 tgtgagcctg ctggtcacat cgcgcaggta cctggactgg cagggcgagc ttggcctgga
     7381 attggaaggc ctgcagcccg atgaaggagc aaagctcttc cggcagagcg cgccgcgccg
     7441 ctcgcatgac attgaacctg agttggctga ggaactcagc cgcaaagtag caggccatcc
     7501 cctcagcttg cgcctgctcg gtggagcgtt caatcaaata acactctctc tgcctgaatt
     7561 catccacgat tgtgatacgc acctgctggc agccgaagat aaatatatcg atcttggaca
     7621 tcgccagcgc acgctctacg ccagtatcga gaccagcgtg cgttacctgg acaccgccct
     7681 ctcgcaactc ttcagcaggt tatgggtgtt ccacgcgcct ttcctgcctc aaagcgccgt
     7741 ggccatctat gatccagatt ttgataatac aaaaggtgga cgttctccca tctacgatca
     7801 actgcatgcg ctctggttgc gcggcctgct cacgctccag cgtgtaaccc tgcgtgaggg
     7861 gacatgggag ttttatagcc tgttgcccac gatgcgccct tacattgaga attacctggc
     7921 gcgagcagaa gaacgcgaag agctgctggc gcgctttggc gcagcctatg cccggctggc
     7981 gcgttttctt tataacgagt tgaatcgtgg aggcacagcc gctttcatcg ccggacaatc
     8041 ccacgaagac ctggagcgtg gcgcatcctc tgtcacgggt gccgagcagg gtcattactt
     8101 tttagattgg ggctgggtcc tccagagaac gggttacacc cggcgcggcc tgcacctgac
     8161 cgagcaggct cttgagattg gacaggggca agatcggtca ctcgaactgc atgcgctcaa
     8221 caacatggcg ggggtgtaca atgcgacggg gcagccgaag cgggcgttgg agttatacga
     8281 gcaggcctta ccgctcatgc gcgaggtggg agaccgggca ggagaggcag ccaccctcaa
     8341 caacatgggg tgggtgtacc gtaccacggg gcagccgaag cgggcgttgg agttatacga
     8401 gcaggcctta ccgctcatgc gcgaggtggg agaccaggtc ggcgaggcgg cttcactcaa
     8461 tggtctggca agtctcttga cggatatgcg tgaatatgaa gatgcacggg catttattgt
     8521 agagtcaatt cgcctggagc ggcaagtcaa ccacccggca ggcgaggtgg ccgggttagt
     8581 aggtctggca tggctactag atcaacatct caaccggcga caggacgcga tcaacgccat
     8641 gcagcaggct atcgcggtgc tggatgaaac gggtttgcct caagatgctg ccggtcagac
     8701 cagagacatg ttgcagcaat accttgatgc gatgcgccag gctgtagctc tcggacaggc
     8761 cgatcaggca gtgacgatgc ccgctgcgca gctcgaggtg attgtccata acacggtcgc
     8821 tgtcatgacc accgcgcagg agcgccgtgc cgagtggcat acaacgttaa cggatgcatt
     8881 gcagtatgtg cagcagcagg gagccaaacg acaaaacgag gttgactttt ttgctgctgt
     8941 gctggcgctg ctggacgggc aaacttcgtc attgccgggt gatcatccct atgcagctgg
     9001 cctggcacaa atccaggctg gcatcgcggc ggaatcgtcc acgaacttct ttgagatgat
     9061 ggtgatggtg tcggagacgc tggccgttct tggccctgtg ccggaacaat tgcctgagtg
     9121 gcgcgcggcg ttgctgcaag ggaaggagca ggcgatgcag gcagatgagc gggagttggt
     9181 cgcgttgctc gatgcgatag ttggtctact cgacgcggga ggcaatccag cgggattggg
     9241 agcagacctg gccggtgatt acgcacaaat gtggcaggcg ctggtagagg ctctgggtaa
     9301 atgatgggga cgtgggaggt gtggatgggg tgtgcaaccc gacgaagggc ggcgcaagcg
     9361 cccccaccca tcatccatac atcc
//
LOCUS       DASZAK010001007         2515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_201634, whole genome shotgun sequence.
ACCESSION   DASZAK010001007 DASZAK010000000
VERSION     DASZAK010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2515
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201634"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1532)
                     /locus_tag="VGT44_17730"
     CDS             complement(39..1532)
                     /locus_tag="VGT44_17730"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2582703.1"
                     /translation="MKQQELLPTPCHTWAEKLAAIHPDDLSIEDRAALDQHIQTCAAC
                     AAALADYRRMDAAILGLPPVEPLLALPPALQQAFGDLEESGELQQSYSSPPRPVRVQP
                     RASRWVRLASAVAAVLVVTALIGGFAFLFISHRTLVGGAGAGQVIYIASRDSDGTVYA
                     IRPSDGAVYWQYGIGQKLNGDLVASSDSVYASAGAHVYAMRKSDGSRRWTSPTIPGGA
                     FSPMLADGNAVYLSSPDSLYALSQSDGHILWHLKQSSCPNGCAAVFMAVTNGTAFAYM
                     DGLYALRARDGQILWHDPAYPFDSRSFAVTNGKVFAPDERKGLLYILSASNGQLLHTF
                     PLLIKDEPVELLAAGGIVYIDNAGYNLYAIQASDDTVLWQKHSDTFILGLSAADNGGL
                     YFSATTVSVGSIVPVTSSGSPIAGATPVVSSAASNDVYAVNNSDGSLRWHWQPSNNNG
                     GASDMLAIGGDVYLTVGGSLYALSANDGHLLWVINIHATSLTSPVGG"
     gene            complement(1529..2194)
                     /locus_tag="VGT44_17735"
     CDS             complement(1529..2194)
                     /locus_tag="VGT44_17735"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2582704.1"
                     /translation="MFQTIQGDSFDELARENAAHLGDPTSAPDRTGEERASMAQVVGQ
                     ARAGDSQAFSALFQHYNSQICTYLARLVGNDEVGRDLAQETFLRAWQSLPAIRSDEHF
                     KPWLFRIAINAAKSHLRQARRVLWLPWQDNESTEASLAQARLEVAGPEERTGETECVQ
                     KALAHVAPQSRACLLLQLVAGFSQREVAQTLGISEKSVSAYVSRGRERFRREYRRLKG
                     AAE"
BASE COUNT          544 a          771 c          675 g          525 t
ORIGIN      
        1 tcgccttgct gccctcgcca cgctacacta ttaccactct atcccccaac cgggctggtc
       61 agacttgtag cgtgaatgtt gataacccac agtaagtggc catcgttggc cgaaagagcg
      121 tacaggctac cgcccactgt gaggtaaaca tcaccaccta tagccagcat gtctgacgcg
      181 ccgccattat tatttgacgg ctgccagtgc cagcgcagcg agccatcgct gttattaact
      241 gcgtagacat cgtttgatgc cgcgctcgat acaacaggcg tcgcccctgc aataggactg
      301 cccgaacttg tgaccggcac gatagaacca accgacacag ttgtagccga gaagtaaagg
      361 ccgccgttat ctgctgccga caacccaagt atgaaggtat cactgtgctt ctgccacaga
      421 actgtgtcgt cgctggcttg tatagcgtac agattgtatc ctgcgttgtc gatatagact
      481 atacccccag cagcgagcag ttccacaggt tcatctttta tgagaagggg aaaggtatgc
      541 agcagttgcc cattactcgc gctcagtatg tagagcagcc ctttccgctc atccggcgca
      601 aaaaccttgc cattcgtcac cgcgaaggaa cgagagtcga acgggtaggc gggatcgtgc
      661 cagaggatct gcccatcgcg cgcgcgcagg gcgtacaatc catccatgta cgcgaaagct
      721 gttccattcg tcacggccat gaatacagcc gcgcagccgt tcgggcagga tgattgtttg
      781 agatgccaga gaatgtgacc atcgctctgg ctaagcgcgt acagggagtc gggagaactc
      841 agataaacgg cgtttccatc cgccagcatg ggcgaaaacg cgccgcctgg aatagtggga
      901 ctggtccagc gccgcgagcc atcgcttttg cgcatcgcgt agacatgcgc gcccgcgctg
      961 gcataaacac tgtcgcttga ggcaaccagg tcgccgttca gcttctgccc gatgccatac
     1021 tgccagtaca ccgcgccgtc actcggacgt atggcgtaaa cagttccatc gctatctctc
     1081 gatgctatgt atatcacctg gcctgcgcct gccccaccaa ccagggtacg atgggagata
     1141 aagaggaagg cgaaaccacc gatcagagca gtcacgacca gcacggcagc aacagcgctt
     1201 gcaagtctta cccagcgaga cgcgcgaggt tggacgcgca ccggcctggg agggctgcta
     1261 tatgattgct gtagttctcc actttcctcc agatcgccga atgcctgctg taatgcgggc
     1321 ggaagcgcga gcagcggctc aaccggtggc aaccccaata tggcggcatc catgcggcga
     1381 tagtcggcaa gcgcggcggc gcaggcggcg cacgtctgaa tgtgttgatc gagcgcggcg
     1441 cggtcctcta tggacaggtc atcagggtgg atcgcggcca gcttttcggc ccaggtatga
     1501 cacggtgtag ggagtagttc ttgctgcttc attcggctgc tccttttagt cggcggtatt
     1561 cacggcgaaa ccgctcgcgc ccgcgactga cataggcgct gacactcttc tcgctgatgc
     1621 ccagggtctg ggcgacctcg cgctgcgaaa aacccgcgac aagctgcaac aacaggcagg
     1681 cacgagactg cggggcaaca tgtgccagcg ctttctgcac acactcagtc tcgccggtcc
     1741 gctcttccgg cccggcaacc tccagtcgag cctgtgcgag cgatgcttcc gttgactcat
     1801 tatcctgcca gggcagccat agtacccgcc gcgcctgccg cagatgcgac tttgcggcgt
     1861 tgatggctat acgaaatagc cagggcttga aatgctcgtc gctgcgaata gcaggcaggc
     1921 tctgccaggc acgcaggaac gtttcctgag ccaggtctcg ccccacctcg tcatttccga
     1981 ccagcctcgc caggtacgtg catatctgcg aattgtagtg ttgaaatagc gcgctgaacg
     2041 cctggctatc accggcgcgc gcctgcccca ctacttgcgc catcgacgcg cgctcttcac
     2101 ctgtcctgtc cggcgcgctg gtcggatcgc cgagatgcgc ggcattctct cgcgcaagct
     2161 catcgaacga gtcgccctgt atagtttgga acacccgctt cactcctcct tttcgcaacg
     2221 ctttttcttg catctatctc tatgacgcct gagaaccgcc aaaccctgtg atagttttac
     2281 cgcttgcttc ctattttgcc tattgcatcc gggcaattgt cccgttattg tgtagaacca
     2341 taaagatcat ccctccgacc ggaggcacta tgagaggagc cacgcccata tgccaactgc
     2401 cgctaagcta gaacaggtcg aacagaccga aaagagcaat aaaagcaata gtaatattac
     2461 tccattccca actcgcttag aatatcaccc gaccatccat gatcttccat cagag
//
LOCUS       DASZAK010001008         7760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_309679, whole genome shotgun sequence.
ACCESSION   DASZAK010001008 DASZAK010000000
VERSION     DASZAK010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309679"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VGT44_17740"
     CDS             complement(<1..418)
                     /locus_tag="VGT44_17740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582705.1"
                     /translation="MLRFFALKPYAVFLGTCILLLATMIPLVAFAQPAPAAAHAKGYS
                     FFGQAKLVTPGNHTTHAVQLTSSTLLEVQYAGINFDVPGKLTFAGIQTLSTDYNFTHN
                     SCGAGSPRFQINVDGANAFVYIGPPPNYTGCATNTWL"
     gene            complement(659..1216)
                     /locus_tag="VGT44_17745"
     CDS             complement(659..1216)
                     /locus_tag="VGT44_17745"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEV2582706.1"
                     /translation="MNSAFAALRLRLLAFLLDYIWIAGYIGVLIVASISLGFSPLRAA
                     FLKLFADPNSSEITAFLLLVLPVVLYFALFECSHWQATWGKQKMGLLVTTNYRKRPGL
                     ARSLFRSLLKFLPWELTHACLWRIPGWPLAPKAAPSIIVAGLILVWVIVGMYVMSLLI
                     SKTHQTLYDLLAGTRVVKIQSGRII"
     gene            complement(1354..3687)
                     /locus_tag="VGT44_17750"
     CDS             complement(1354..3687)
                     /locus_tag="VGT44_17750"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2582707.1"
                     /translation="MGDLCFILLGPPEVRHADQVLLFSTRKELALLIYLAVERRVHLR
                     KNLSEQFWPEGDAMHGRAALRISLLHLRHLLGEGADGVAAPHLLIQRDTLGLDPTSAL
                     DIDLHTLYEAWTLARASTHTTLTMPEDAHRTLLARLQRATSLPRGEFLEGFSLRDAPA
                     FDDWVRFQREYWHLRTNEVFDRLSQIQFELGELEAAIETTSRWLTLAPLHEDAYRRLM
                     RLHFAAGDRAAALHVYDTCRAMLATGMQSEPTPETVALANRMRAVAPPHRREAPTRPA
                     GLSPAALLDGPLLGRTAELSALIKLYHSARRGRTQVVFLEGDVGIGKTRLATEFLAWA
                     EIEGADVLQGQAFEAGGQLPYRPLIEALRPRIERENAPDDLLSDLWLAELARLLPELY
                     DRYPDLPEPTGDKSVARTRLFEAVVRLGQGLAARAPLVLFIDDVQWADAASLDVLHYL
                     ARRFAESETPALLLLTLRMGERETRPEIAEWRTGLERAAPLTRLQLGPLTSEDILRLL
                     QVLGGKDGRRPADLERFGQWLFTETEGQPFYLMETLKVLLEQSILASHANEDGGWTID
                     FTAAMQHEAMVRGFFPPSVREVIVARLDRLTPNASALLVAGAVLGQGTTFEHMCQVAD
                     LAEQDGLPALDEALHSHLLQESEREGGYMIHGRYVFAHAKIRAVVYAEAGEARRSIFH
                     RRALNVLQAAAAPPAILAYHAIAAGLAVLAFRFSLAAGDEAMRVVAVRDAITFYEQAQ
                     HLLTEPVQSLSLEATLPAPEIEHLYTNLGRAYELNAE"
     gene            3859..4341
                     /gene="dps"
                     /locus_tag="VGT44_17755"
                     /gene_synonym="pexB"
     CDS             3859..4341
                     /gene="dps"
                     /locus_tag="VGT44_17755"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEV2582708.1"
                     /translation="MNRKNNALPGEQQAKLIELLNRRLADAIDLQLQSRQAYWNVKGP
                     HFIALRALFDKIVEGVEEYANLIAERIVQLGGMAEGTAHAVAGRSSLDRYLLATADGN
                     GHISALSTTLINFGRHIRYASEQASELKDADTAAIFTEIGRGIDKWLWFVETSQQPGS
                     "
     gene            4583..4765
                     /locus_tag="VGT44_17760"
     CDS             4583..4765
                     /locus_tag="VGT44_17760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582709.1"
                     /translation="MQTTRTYTTGMFRGEARDQLGKDVKKMAKAGWSVHTVTDEGVGK
                     GSNHTGRLTVLYEKKE"
     gene            complement(4770..5300)
                     /gene="dps"
                     /locus_tag="VGT44_17765"
                     /gene_synonym="pexB"
     CDS             complement(4770..5300)
                     /gene="dps"
                     /locus_tag="VGT44_17765"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HEV2582710.1"
                     /translation="MVEIVAHAADQEKINRMYKTQNSLPEEQRIKLVELLNRRLADGI
                     DLMLQAKQAHWNVKGPQFITLHTLFGQIANGVEQYIDQLAERVVQLGGSAEGTAYLVT
                     RRTSLDRYPAAIVDGNGHTIALAAVLASFGKHARYAIGQAADLLDADSADLFTEISRG
                     TDNWLWLVEAHLQAAQ"
     gene            5484..>7760
                     /locus_tag="VGT44_17770"
     CDS             5484..>7760
                     /locus_tag="VGT44_17770"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2582711.1"
                     /translation="MPGSPEWSNLHFALLGPPQVYDGGRLLVFPSRKSLALLIYLAVE
                     GGVHSRKMLSELFWPESDAVHARGTLRTTLLELREVLAEGTSPADGAGPDRIPHLLID
                     RDTLGLDLTSGVSQDLQALLAAWELARDASRTPVKLSEEARREQRLQLQGATRLLRGE
                     FLAGFSLHNAPGFDDWARFQREYWHLRMHLVFERLSQWYEEAGEIEQAIATIIRWLAL
                     NLLNEDASQRLMRLHFATGNRVAALQAYETLRAALASEIHAEPTPETMALAERIRVTA
                     PPHHHEKQRSPLISSTPLAMLHAPLVGRTREYGILLERYHAARSGQAQVVLIEGDAGI
                     GKTRLATEFVDWASAQGADVLRGQAFESSGRLSYQPLIEALRPRIDQENAPDDLLHDV
                     WLAELARLLPELRERYPDLSGPVADASVARNHLFEAVARLLDALAARAPLLLFLDDLH
                     WADTATLDLLHYLARSFTERATPALLLLSLRTEALQTTPPLAEWRANLGRIVSLTRLQ
                     LGPLSAQDTLHLLQVLFGADRRGAATRSRASVLGEQPSADALERVGERLFAETGGQPF
                     YLVETLKLLLERGHFALSEDGAKAGDVAAAMVKELDVPRLFPPSVRELVSLQLDRLSP
                     AAFAFLVAGAVLEQDATFERLCLVADLSESEGLAALDEVQRSRLVREPERQSGQPGNA
                     AYAFTHDMIREVVYVEAGETRSRIFHRRALDSLQEASVAPAKLAYHAVSAGLLEPAFR
                     LNMAAGNESMAVFAFHDAI"
BASE COUNT         1656 a         2217 c         2300 g         1587 t
ORIGIN      
        1 tgagccaggt gttcgtggcg catcctgtat agttaggcgg cggtccaatg tagacgaaag
       61 catttgcgcc atcgacattg atctggaagc gcggcgatcc agccccacaa ctattatggg
      121 tgaagttata atccgtactg agtgtttgta taccggcaaa cgttaatttc cctggaacat
      181 caaagttaat gccagcatac tgcacttcca ataaggtgga tgacgttaac tggaccgcgt
      241 gtgtcgtgtg gttccctggc gtaacgagct tcgcttgacc aaagaaagag taccccttcg
      301 cgtgagcagc tgccggagct ggctgagcga aggccactag aggaatcatc gtcgcgagca
      361 gcaaaataca cgttcctaaa aagacggcgt aaggtttgag cgcaaagaaa cgcagcatcg
      421 agttccttcc ttcctgttgt tgaactggtt tgactcagct catctactgc aaatcaaaat
      481 gagatactaa aacaagataa cccatggtag tcaatttgtc aagcagacgt acaactatcc
      541 ttatcactta tgaaagcata ttcgatttct attcaatcaa atgccaggat ttattgatga
      601 aattccggag attttgaaca cttgcgcaga agcgttggaa cagagtggtt ctttgagatc
      661 aaattatgcg gcctgattgt attttgacaa cacgtgttcc tgcaaggaga tcatagagtg
      721 tttgatgagt cttactaatg agtaagctca ttacgtacat tccgacaatc acccagacga
      781 gtattaaccc agccacgata attgatggag cagcctttgg agccaggggc cagcctggaa
      841 tgcgccagag gcaggcatgc gttagctccc atggcaagaa tttcagcaag gaacgaaaca
      901 gcgatcgtgc caaacccggt cgctttcgat agtttgtggt cacaagcaat cccatcttct
      961 gtttgcccca ggtggcttgc caatgtgagc attcgaacag cgcgaagtag agtacgaccg
     1021 gcagaaccag caagagaaaa gctgtgatct cgctagaatt gggatctgca aacagcttca
     1081 ggaaggctgc tctaagaggg ctaaacccga gcgagatact tgcaacgatg agtacgccta
     1141 tgtaccccgc gatccatatg taatctagca aaaatgccag taggcgcagt cgaagggctg
     1201 caaacgcact gttcatctaa cttcaccttt ttaatgtcgt ctcccggaag tggcggcatg
     1261 ctctttttgt tatatctact ggtatactaa ccacgcagcg ttaaaaagag cgttaaaaca
     1321 gaaggaatag atctaagtcg ttcgagtctt cttctactca gcgttcagtt cataggctct
     1381 gcccaggttt gtataaagat gttcgatctc cggtgcggga agcgtggctt ccaggctgag
     1441 cgattgcacc ggctcggtca atagatgctg ggcctgctcg tagaaggtga tggcatcgcg
     1501 cacggctacc acccgcattg cttcatcgcc cgccgcgaga ctgaagcgga aggccagtac
     1561 cgccagcccc gcggcgatgg catgatatgc caatatcgcc ggaggtgcag cagccgcttg
     1621 caacacgttc aaagcacggc ggtgaaagat ggaccttctt gcctcaccag cctcagcgta
     1681 gaccaccgca cggatcttcg catgtgcgaa cacgtagcgc ccatggatca tgtacccccc
     1741 ttcgcgctcc gattcctgga gcaggtggct gtgcagtgct tcgtccaggg caggaagtcc
     1801 atcctgttct gcgagatcgg ccacctgaca catgtgttca aaggtggtgc cctggccgag
     1861 gacggctccg gccaccagca acgctgaggc gttcggtgtg agtcgatcca accgtgccac
     1921 gatgacttcg cgcacacttg gcgggaagaa cccacgtacc atcgcctcat gctgcatagc
     1981 tgccgtaaag tcaatggtcc atccgccatc ttcgttcgcg tgggaggcga gtatactttg
     2041 ctcgagcaag accttgagcg tctccatcaa atagaagggc tgcccctccg tttcggtaaa
     2101 cagccactga ccaaaacgtt ccaggtccgc cggtcgcctc ccgtctttgc cccctaagac
     2161 ctggagcagg cgcaggatgt cttcgctggt gagggggccg agctgcaggc gggtcagcgg
     2221 cgcagcgcgt tccaaaccgg ttcgccactc ggcaatctct ggcctcgtct ctctctcccc
     2281 catgcgcagc gtcaggagca ggagcgcagg cgtttcgctc tccgcgaagc gccgtgcaag
     2341 gtaatgcaac acgtcgagcg aggcggcatc ggcccactgc acatcatcga tgaacaagac
     2401 cagcggcgct cgtgcagcca ggccctgtcc caggcgtact actgcttcga acaagcgggt
     2461 gcgcgccacg gacttatctc ccgtcggttc aggtaagtcg ggatagcggt catacagttc
     2521 ggggagcagg cgcgcgagtt cggctagcca cagatctgaa aggagatcgt ccggtgcgtt
     2581 ctcccgctcg atgcgcgggc gcaaggcctc gatcaggggc cggtagggca gctggccacc
     2641 ggcttcaaaa gcctgtccct gtagtacgtc tgctccttct atctctgccc aggccaggaa
     2701 ctcggtcgcc agccgcgtct tgccaatacc gacatctcct tccaggaaca caacctgcgt
     2761 ccgaccgcgt cgtgcgctgt gatagagctt gatcaacgcg ctcaactccg ccgttcgtcc
     2821 caggagcggg ccgtcgagga gcgctgctgg ggaaagtcct gccgggcgcg tgggagcctc
     2881 cctgcgatgg ggaggagcga cggctcgcat acggttggcc agcgccacgg tttccggggt
     2941 cggctcgctc tgcatgcccg ttgccagcat tgcccggcag gtgtcataga cgtgcaatgc
     3001 agcagccctg tcaccagccg cgaagtgcaa gcgcatcaga cgccggtagg cgtcctcatg
     3061 cagaggggcg agggtgagcc agcggctggt cgtttcaatg gcggcctcaa gctctcccaa
     3121 ttcgaattgt atctgggaga ggcggtcgaa gacttcattc gtgcgcaggt gccagtactc
     3181 gcgctggaag cggacccagt cgtcgaaggc cggggcatcg cgcagcgaaa agccctccag
     3241 gaactcgcca cgagggaggc tggtcgcgcg ttggagccgg gccagcagag tacggtgcgc
     3301 gtcttctggc atggtcaggg tcgtgtgagt cgacgcgcgc gccaatgtcc aggcttcgta
     3361 gagagtatgg aggtcgatgt cgagagcgga ggtagggtca aggccgaggg tgtcgcgttg
     3421 gatgaggagg tggggtgcgg caacaccgtc agcgccctca cccagcagat gacgcaagtg
     3481 gagcaggcta atgcgcaagg ctgcccggcc atgcatcgcg tccccttctg gccagaattg
     3541 ttcggagaga ttcttccgca agtggacgcg gcgttccacc gccagataga tcagcagcgc
     3601 gagttccttg cgcgtggaga acagcagcac ctggtcggca tggcgcacct cgggtggacc
     3661 caacaagatg aagcataagt cgcccattta acggtcctta taacgctctg ctgctatcat
     3721 gagatcagag atgctatagg tgaagaaaac tgactccaca tgcatgaaca aaagggtgtc
     3781 agagagtttc aggtcaacta cgacatataa ccaatccatt atcgcacaaa acgcggcttg
     3841 agtggaagga gcgctcccat gaacaggaaa aacaatgctc tgcctggaga acagcaggcc
     3901 aaactgatcg agttgttgaa ccgccgctta gccgacgcca tcgatctgca gttgcaaagc
     3961 aggcaagcct actggaacgt taaggggcct catttcatag ctctgcgcgc gctgttcgac
     4021 aagattgtcg agggagtgga ggaatatgcg aacctgattg ccgagcgcat cgtacagctt
     4081 ggcggcatgg ctgagggaac ggcgcacgcg gtcgccgggc gttcttcgct cgacagatat
     4141 ctgctggcaa cggcagacgg gaacggccat atcagcgccc tttcgacgac actcatcaac
     4201 ttcggcagac atatccgcta tgccagcgag caggcgagcg aactgaagga tgccgatacg
     4261 gctgccatct tcaccgagat cgggcgaggc atcgataagt ggctctggtt cgtcgagacg
     4321 agccagcaac caggcagttg agaggctatg tagtatcttt tcctgccgtc atatcttact
     4381 cactgtaagc aagaaggacg gcaggaatat accagtaaag gacatcgtta tgccaccatt
     4441 tgtatcgctg tttctgcttt ttgcgttctt tataggctcg ctgtggttcc atgacgtctg
     4501 gtttccaccc gaggggcgtg ggtacaaaaa gaaacgaatc cacggccata gtcaagtcta
     4561 gcagcagagg agaagtgaga ccatgcaaac caccaggacg tatacgacgg gaatgtttcg
     4621 gggagaagcg cgcgatcaac ttggtaagga tgtcaagaag atggcaaaag cgggctggag
     4681 tgttcatacc gtcaccgatg aaggagtcgg gaaaggttca aatcacaccg gtcgattgac
     4741 cgtattgtat gagaagaagg agtagtgcat cactgcgctg cttgcaggtg ggcttcgacg
     4801 agccagagcc agttatcggt gccgcgcgag atctcggtga agagatcggc gctatcggca
     4861 tcgagcagat cagccgcctg tccgatagcg tagcgggcat gtttgccaaa gctggcgagt
     4921 accgccgcaa gcgctatggt gtgaccgttc ccgtcaacaa tcgcggctgg ataccggtcg
     4981 agcgacgttc gccgggtgac caggtacgcc gtcccttcgg cactgcctcc caactgcaca
     5041 acacgctcgg cgagctggtc aatgtactgc tcgactccgt tcgcaatctg gccgaacagc
     5101 gtgtgcaggg tgatgaattg cgggcctttc acgttccagt gggcctgctt ggcctggagc
     5161 atcaggtcga tgccgtcagc taggcggcgg ttcaacaact cgaccagttt gatgcgctgc
     5221 tcttctggga ggctattttg tgttttatac atgcgattta ttttctcctg atcagcagca
     5281 tgtgcaacaa tctccaccat ctctgcttag agtgtaatcg cagagcgtta ggaaaagcgt
     5341 tagtgcagcc gtgaaaaagc ttcttgttct ttccatcatt gacttcgcac gacaggggtt
     5401 gtagtataaa gaccaagcgc agagagggct atgctgcgta ttgtagcaga gcgataatac
     5461 ttacgagagg taggaaattc gctgtgcctg gatcaccgga gtggagcaat ttacacttcg
     5521 ccttgcttgg cccgccgcag gtctacgatg gcggtcggct gctcgtattc ccttcccgca
     5581 agtccctcgc gctgctcatt tacctggccg tggagggcgg ggtgcattcg cgcaaaatgc
     5641 tctcggaact gttctggcca gagagcgacg cagtacatgc tcgagggact ctgcgcacga
     5701 cgttgctcga gcttcgagaa gtcctggctg agggaacttc cccggcggat ggagccgggc
     5761 cagatcgcat accgcacctg ctgatcgatc gtgacaccct gggcctggat ttgacctctg
     5821 gtgtcagcca ggacctgcaa gctctgctgg cggcctggga actcgcacga gacgcctctc
     5881 gcacgcctgt caagctctca gaggaggcac gtcgcgagca gcgtctacaa ctccaggggg
     5941 cgaccaggct gttgcgtggg gagttcctgg ctggtttctc gctgcacaac gccccgggct
     6001 tcgacgactg ggcccgcttc cagcgcgaat actggcatct gcgcatgcac ctggtcttcg
     6061 agcggctctc tcagtggtac gaagaagccg gcgagatcga acaagccatc gccaccatca
     6121 tccgctggct cgccctcaac ctgctgaatg aagatgcctc gcagcgcttg atgcggctgc
     6181 acttcgcgac cggcaaccgc gttgctgcgc tgcaagcgta tgagacccta cgggcggccc
     6241 tggcctcaga gattcacgcc gagccgaccc cggaaacgat ggccctggct gagcgcattc
     6301 gggtcaccgc tcctccccat caccacgaga agcagcgctc cccattgata tcgtccacgc
     6361 cgttggcgat gctccatgct ccactggttg gacgaacgag ggagtacggc atcttgctcg
     6421 agcgctacca cgcagcccgc tcggggcagg cgcaggtggt acttatagaa ggggatgctg
     6481 gcatcggcaa gacgcggctg gccaccgagt ttgtggattg ggcaagcgcg cagggagccg
     6541 atgtgctgcg cggtcaggca tttgaaagca gtgggcggct gtcctatcag ccgctcatcg
     6601 aggccttgcg gcccaggatc gaccaggaaa atgcccctga cgatctgctg catgacgtgt
     6661 ggctggctga actcgcgcgc ctgttaccag aactgcgcga gcgttatccc gacctctccg
     6721 gcccggtagc agacgcctcg gtggctcgca accacctgtt cgaggcggtc gcccgccttc
     6781 tggacgccct ggcggcacgc gccccgctgc tcctcttcct cgatgacctg cactgggccg
     6841 ataccgctac actggacctg ctgcactatc tggcacgaag cttcaccgag cgcgctacgc
     6901 cagcgctgct gttgctctca ctgcgcaccg aggcgctgca gactactcct ccccttgctg
     6961 agtggcgagc gaacctgggt cggatcgtct ccctgacacg cctgcaactg ggtccactct
     7021 cggcccagga taccttgcac cttctacagg tcctgttcgg cgcagaccgg agaggggcgg
     7081 cgacaaggtc gcgggcttcc gtcctgggcg agcaaccgtc agccgatgcc ctggagcgcg
     7141 ttggggagcg gctctttgca gaaacgggcg gacagccttt ctacctggtc gaaacgctca
     7201 agctgctgct tgagcgcggt cattttgccc tgagtgaaga cggcgcaaaa gccggtgacg
     7261 tagcggcagc gatggtgaag gagcttgatg tgccacgcct cttcccccca agcgtgcgcg
     7321 aactcgtcag cctgcagctc gatcgactta gcccggctgc ctttgccttc ctggtagccg
     7381 gcgccgtgct ggaacaagat gccacctttg agcggctctg cctcgtagcg gatctctccg
     7441 aaagtgaggg gctggctgcg ctggacgaag tgcagcgcag ccgcctggtg cgcgagccgg
     7501 agcggcagag cggacaacca ggcaatgcag cctacgcctt cacccacgac atgattcgcg
     7561 aggtggtcta cgtcgaagcg ggcgagacgc gaagccgtat ctttcaccgg cgcgcgctag
     7621 acagcttgca agaagcttcc gtcgctccgg cgaaactggc gtaccatgcc gtgtcggcag
     7681 gactgctaga accggcattc cgcttgaata tggcggcagg gaacgaatcc atggccgtct
     7741 ttgctttcca cgacgccatc
//
LOCUS       DASZAK010001009         2816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_131219, whole genome shotgun sequence.
ACCESSION   DASZAK010001009 DASZAK010000000
VERSION     DASZAK010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131219"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..551)
                     /gene="minE"
                     /locus_tag="VGT44_17775"
     CDS             complement(201..551)
                     /gene="minE"
                     /locus_tag="VGT44_17775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEV2582712.1"
                     /translation="MGVFEFMIGRKKQSTGQVAKERLKVALANDRIKINPELLEMIKG
                     DLLKAISQRLEVDEQHVQVNMSREGGFDRLLADMPVKRQKIAFEWDPPAYTPAANALK
                     GNSLHVEVERLADE"
     gene            complement(578..1405)
                     /gene="minD"
                     /locus_tag="VGT44_17780"
     CDS             complement(578..1405)
                     /gene="minD"
                     /locus_tag="VGT44_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEV2582713.1"
                     /translation="MDSRVITITSGKGGVGKTTTTANLGTALAMMGKKVAVVDSDIGL
                     RNLDAVMGLENRIVYDLVDVVEGQCRLRQALIKDKRQPDLFLLPAAQTRDKNAVNSVQ
                     MEQLCAQLRQEFDYIIIDSPAGIEQGFRNAIVGADEIIIVANPEMASVRDADRIIGLV
                     EAAGKPEPRLVLNRLRAEMVRRGDMMDVADVLEVLGIDLLGIIPEDETIIVATNKGEP
                     IVYDKRARAGRAYLSAAQRILGEEVPLEEVEESPTLMDRFRRLMGIGPAMDSRRVRS"
     gene            complement(1463..2182)
                     /gene="minC"
                     /locus_tag="VGT44_17785"
     CDS             complement(1463..2182)
                     /gene="minC"
                     /locus_tag="VGT44_17785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEV2582714.1"
                     /translation="MQGYIAIKGTRNGLLLTLEPGTPFSDLLTALSERLAEAPSFFRG
                     GSLSLDTTRRSLEVGERTQLEALLAFYQMSVAAAEPSSALPVEAEPLLALPEALSAQT
                     ESANGVLSEAGSLDEIASDPRESSNTLFLRRTVRSGQAIKHPSNVVVLGDVNHGAEIV
                     AGGDIIVWGVLRGMVHAGFPDNEQALVCSLALAPVQLRIAHLLSRPPDGFEMQPRAEV
                     AAIRNDQIVVEAWINGRPPRK"
     gene            complement(2415..>2816)
                     /locus_tag="VGT44_17790"
     CDS             complement(2415..>2816)
                     /locus_tag="VGT44_17790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582715.1"
                     /translation="ELLRELGVTPEESGASLRPTSYSTSALSPFTERMILEHIAEAAT
                     EDAEAWLEDLDTMRREVDSIVRQVIRLIKRGGLEPSLKEDVMVVLRALRSRASATRPA
                     IDGEIASIESNAAMLHFCRIILRLSETTVEE"
BASE COUNT          562 a          828 c          820 g          606 t
ORIGIN      
        1 gggtacgggt acagtacaac tggaattcta cggcaaggct atctgtaaag caggccaggc
       61 ctgtccgcag tatatcatgc gtttgccgtt tacgattgcg gtaaagtaaa tgtttgccag
      121 ggcaagggcg aagccaggta acggcgaagc cagggcgatc ctcgcgatcg ccctggcttc
      181 gccgttacct ggcttctctg ttattcgtcc gcgagccgtt cgacttcgac gtgcagcgag
      241 ttacctttga gggcgttagc tgcgggggta taggctggcg ggtcccactc aaacgcgatc
      301 ttctggcgtt tgacgggcat atccgccagc aagcgatcga agccgccctc gcgcgacatg
      361 ttgacctgca cgtgctgctc atcaacctcc aggcgctgtg agatggcttt gagcagatcc
      421 cctttaatca tttccagcag ttccgggttg attttgatgc gatcgttcgc cagcgctact
      481 ttcaagcgtt ccttcgccac ctggcccgta gattgttttt ttcgaccaat cataaactca
      541 aacacaccca ttgctttcgc ctcctccccc tggtcggcta cgaacgaaca cgcctgctgt
      601 ccatggccgg gccaatcccc atcaaccgcc gaaagcgatc catcagcgta ggcgattcct
      661 ccacctcttc gagtggcact tcctcgccca ggatgcgttg agcggcgctg agataggccc
      721 gaccggcccg cgcgcgtttg tcgtagacaa tcggctcacc tttgttggtg gcgacaataa
      781 tcgtttcgtc ttccgggatg atgccgagca aatcgatgcc aagcacctcc aagacatcgg
      841 ccacgtccat catatcgccg cgccgcacca tttcggcgcg cagccggttg agaaccaggc
      901 gcggctccgg tttgcctgcc gcttcaacca ggccgatgat gcggtcggca tcgcgcaccg
      961 aggccatctc tggattggcg acaatgatga tttcatccgc gccgacgatg gcattgcgaa
     1021 agccctgctc gatgcccgcc ggcgagtcga tgatgatata gtcaaattcc tggcgcagct
     1081 gcgcgcagag ttgctccatc tggacgctat tgacggcgtt cttgtcacga gtctgggcag
     1141 cgggcagcag gaaaaggtcc ggctggcgct tatccttaat gagggcctgg cgcaggcggc
     1201 actggccctc gaccacatcg accaggtcat agacgatgcg gttctccaat cccatgacgg
     1261 catccaggtt gcgcagcccg atatcggagt cgacgaccgc gacctttttg cccatcatgg
     1321 ctagcgctgt ccccaggttt gctgtggtgg tggttttgcc aacaccgccc ttccccgatg
     1381 tgatagtgat gacccggctg tccataaacg tctccatgta agcaactacg attgtgacaa
     1441 tgccgaaaga gtgaaatacg aatcatttgc gtggcggtcg gccattgatc caggcttcta
     1501 ccacgatttg atcgttgcga atggccgcga cttcggcgcg tggctgcatc tcgaatccat
     1561 caggtggacg cgagagcagg tgagcgatgc gcaactgcac gggggccagc gcgagcgagc
     1621 agacgagggc ctgctcgttg tcgggaaagc ccgcgtgaac catgccgcgc aggacgcccc
     1681 agacgatgat atcgcccccg gccacgatct ccgcgccgtg gttgacatcg cccagcacaa
     1741 cgacattgct gggatgcttg atggcctggc cggagcgtac cgtgcggcgc aagaaaagcg
     1801 tgttgctcga ctcgcgcggg tctgatgcaa tttcatccag cgaacccgcc tcgctaagca
     1861 cgccgttcgc cgactctgtt tgcgcggata gagcctcagg tagagccagg agtggctcgg
     1921 cctcgaccgg cagcgcggag gatggttccg ccgcggcgac cgacatctgg taaaaggcga
     1981 gcagcgcttc aagctgggta cgctccccaa cttcgagcga gcggcgcgtg gtgtctagag
     2041 agagagagcc gcccctgaag aagctgggcg cctcggcgag ccgctccgaa agggcggtaa
     2101 gtaaatcact aaagggtgtc cctggttcca gggttaaaag caggccattg cgggtacctt
     2161 tgatggcgat atatccttgc actgactgcc tctcctccct ttccagtcca ccccttgctt
     2221 cggcctgttg atccgaagta tcctccattg tcatacgtac tgtgaccatc ccgttgctgg
     2281 ataaagggtg aatccaatga cacgttaagc agaccctgca ttccatcccg acgggcatag
     2341 aaccttttat caagataaag tataatcacc tggggaataa aacgcaatag caataggcaa
     2401 tgtggtatgc tttgctactc ctcaacagtg gtttcgctga gacgcaaaat aatgcggcag
     2461 aagtgcagca tggcggcatt ggactcgatg gaggctattt ctccatctat agccgggcga
     2521 gtggcggagg cgcgactgcg cagggcgcgc agtacgacca tcacgtcctc tttgagggat
     2581 ggttccaggc cgccgcgttt gatgagccgg atgacctggc gcacgatgga atcaacttct
     2641 cggcgcatgg tgtcgaggtc ttcgagccag gcttccgcgt cttctgtggc ggcttcggcg
     2701 atatgctcta aaatcattcg ctcggtgaat ggtgagagcg cggacgtgct gtaactggta
     2761 ggtcggaggc tagccccaga ttcttccggc gtgactccta gctcacgcag caactc
//
LOCUS       DASZAK010001010         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_98467, whole genome shotgun sequence.
ACCESSION   DASZAK010001010 DASZAK010000000
VERSION     DASZAK010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98467"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..270)
                     /locus_tag="VGT44_17795"
     CDS             complement(55..270)
                     /locus_tag="VGT44_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEV2582716.1"
                     /translation="MSIHPFFDYIHILFLVGCVFWVWAIYDCATHEPSTGNDKIAWLL
                     VIIFLNVLGAFLYFFIRRPERIRMFGR"
     gene            complement(545..1438)
                     /locus_tag="VGT44_17800"
     CDS             complement(545..1438)
                     /locus_tag="VGT44_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582717.1"
                     /translation="MEQVERFYRVWIPLLHYVNEQRDLVAPFPPDSKEARLPVSDIFK
                     LRDALWADDGLRENYIATNPFKIAPADLALIESWRYRVSGNFYILRYLKKYTVFLSEK
                     ASGSAYGVLGLVSPIEEVIGPALPVYVRAVLLPFENHIIYDSVLSPYSIYFGPGIRSS
                     LNDNYRDAQEREGIITNLLPVDAPAGSQEERKGILARNARIMQAFRRDMTRRGLSHSM
                     TDQHAGNIDAFARDFLLTQDPPRGLLQLTPTDAQTYLQEVGSKTTITSFKRFVRFLTD
                     SGRLDYAYAESLRDILKQVGD"
     gene            complement(1425..1652)
                     /locus_tag="VGT44_17805"
     CDS             complement(1425..1652)
                     /locus_tag="VGT44_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582718.1"
                     /translation="MSQGDGQHRPGFSLAIPWLLSHLHPGFSLAFALAVVQQPCAPIS
                     ASCHQATTKIYYFTGQKIFWSIRYAVVNGTG"
     gene            complement(1658..2371)
                     /locus_tag="VGT44_17810"
     CDS             complement(1658..2371)
                     /locus_tag="VGT44_17810"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV2582719.1"
                     /translation="MSTIWETVTWNVARAGGFTSYILLTLAVAVGLALSTQLQSPSRW
                     PRLLNSELHNFLTLLSSIFIIVHVLAVWIDPFTRFGWNEVFIPFVSTYRTVWMALGIV
                     GLYLGIAIGISTWLRPHIGYKWWRRLHVLTLGIYALATLHGIGTGSDTQTWWAFAIYA
                     VSIVLVGSLLWRRIVETKNKNKKKAHVAAAAQRAARPAQRSNASYPANPRTMNSAAAN
                     EIAPARTQAHYQRDAARVR"
     gene            complement(2434..2841)
                     /locus_tag="VGT44_17815"
     CDS             complement(2434..2841)
                     /locus_tag="VGT44_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582720.1"
                     /translation="MLAIALAKHTITLEKHTMNTMNPYETNTLSQKPKPPQKMSKARA
                     MELAGKMKRWLVVASFVGFGSFSGLAASHIVGTTSTASTSHTSSTSQKSSGSSSSNIL
                     NQSSSSNFGSSSSSSSSSSSSSSSTVSSGTSVA"
BASE COUNT          753 a          823 c          858 g          740 t
ORIGIN      
        1 gtggtcgccc tctgaaaacc tcaacaatgc aaaattttta cggataccct tgagctatcg
       61 tccaaacatt cttatacgtt ctggacgcct gataaaaaag tagaggaatg cgcccagcac
      121 atttaaaaag atgatgacca gcaaccaggc gattttgtca ttgcctgtgg aaggctcgtg
      181 cgttgcgcag tcatagatcg cccagaccca gaacacgcat ccgacaagaa aaagaatatg
      241 aatatagtcg aagaacgggt ggattgacat agcgtttctc ctcagggacg ataaaataaa
      301 caagctctgc tcttattaac gttaacgtgc gtcctacgtc aaacggcgcg gttggcgcct
      361 tcttccttta tgaagaggtg acataacgaa tatcacaacc acgatcggtg ctgtcatgct
      421 gagcgcagcg aagcatctcg cggtccattg atcgagaccc ttcgctgcgc tcagggtgac
      481 acatgatgag cgagtttggt tgtaaaagtt ccttatcgaa ggaaatcgtt ctatgaaata
      541 ccaactaatc gccgacctgc ttcaatatgt ctcgcagcga ctctgcatat gcataatcca
      601 ggcgtcccga atcagttaga aagcggacga aacgcttgaa gctggtgatc gttgtcttgc
      661 ttcccacttc ctgtaagtag gtttgcgcat ccgttggcgt caattgaagc aatccacgcg
      721 gtgggtcttg cgtcaggaga aaatctctgg cgaatgcatc gatgttcccg gcatgctggt
      781 ctgtcattga gtgacttaat cctctcctgg tcatgtctcg ccggaaagcc tgcataattc
      841 tggcgttgcg cgcaagaatg cctttgcgtt cctcctgcga accggcgggc gcgtcaactg
      901 gcaggagatt tgtgatgatg ccctcgcgtt cctgcgcatc tcgataatta tcattcaaac
      961 tgcttctgat gccaggacca aagtagatgc tgtacggcga taagacgcta tcgtagatga
     1021 tgtggttctc aaatgggagc aataccgctc gaacatagac gggaagcgcc gggccaataa
     1081 cctcttcaat tgggctgact aatcccagca cgccataggc gcttcccgac gccttctcgc
     1141 taagaaagac ggtgtatttc ttcaggtagc gcaaaatata gaaattccca gagacgcgat
     1201 agcgccagct ctctatgagc gccaggtcag ccggtgcaat cttaaaggga ttggtcgcaa
     1261 tgtagttctc tcgcagaccg tcatccgccc acaaagcatc gcgaagtttg aaaatgtcgc
     1321 tcaccggcag cctggcctcc ttcgagtctg gaggaaatgg agccaccagg tctcgctgct
     1381 cattgacata gtgaagcagc ggtatccaga cacggtaaaa acgttcaacc tgttccattg
     1441 acaactgcat agcggatact ccaaaatatt ttttgccccg tgaaatagta tatctttgtt
     1501 gtggcctggt ggcaactggc tgaaattggc gcacagggct gttgcacgac ggccagggcg
     1561 aacgcaagcg agaagccagg gtggaggtgc gagagaagcc aggggatggc gagggagaaa
     1621 ccagggcgat gctggccatc gccctggctc atactctcta cctcacgcgc gccgcatccc
     1681 tctgatagtg tgcctgcgtt ctcgcgggag ctatctcgtt cgcagcagcg ctgttcattg
     1741 tgcgagggtt ggcgggatag gatgcattgc tgcgctgggc gggccgcgcg gcgcgctgtg
     1801 cggcggcggc cacgtgcgcc ttcttcttgt tcttgttttt cgtctctacg atgcgccgcc
     1861 agagcaggga gccgaccagc acgatgctga cggcgtagat ggcgaaggcc caccaggtct
     1921 gcgtatcgct gccggtgccg ataccgtgca gcgtggcgag agcgtagatg cccagcgtca
     1981 ggacgtgcag cctacgccac catttatagc cgatgtgcgg gcgcagccag gtgctgatgc
     2041 cgatggcgat gccgaggtac agtcccacga tgccgagcgc catccagacc gtgcgatagg
     2101 tgctcacgaa cgggataaag acttcgttcc agccgaaacg ggtgaagggg tcgatccaga
     2161 ccgccaggac atgcacgatg ataaagatac tgctcaataa tgtcagaaag ttgtgtaatt
     2221 cgctattcag taggcgcggc caacggctgg gcgattgcaa ctgtgtcgaa agcgccagcc
     2281 ctaccgcgac cgccagggtc aataatatat aagaagtaaa tccgcccgcg cgcgccacgt
     2341 tccacgtcac tgtttcccag atggtactca tgatcttcct ccatgtcttt tctaaatcag
     2401 aattgatgat ggctgattcc cggcaacaac gggctacgca acgctcgtgc cgctgctaac
     2461 cgtgctactt gacgagctac ttgacgaact gcttgaagaa ctgctcgatc caaagttact
     2521 gctgctcgac tggttgagga tgttgctgct gcttgatcca ctgctctttt gtgacgtaga
     2581 cgatgtgtga ctcgtcgatg ccgtcgaagt agtgccaacg atatgtgatg ccgccagccc
     2641 gctaaaactg ccgaagccga cgaacgaggc gacgaccagc cagcgtttca tctttccggc
     2701 cagctccatc gcccgcgcct tcgacatctt ctgcggcggc ttcggcttct gggataatgt
     2761 attggtttcg tatggattca ttgtattcat cgtatgtttc tccagggtga tggtatgttt
     2821 tgccagggcg atggcgagca tcgcccctac ccttttttta gatgttcggg aggatttccc
     2881 ttacatgtgt aagcatagga tatcaaggtt gaaaatttat tggaaacgca tgagaaacct
     2941 ctaatattga cgcgctgtgc tatcctcaaa gataaagcag atatacgaat tgtgtgtagt
     3001 caaccaccag ccgtagtagt ccgtcacggt tcaaatggcg tgtaggggcg caattatgaa
     3061 ctttaacaaa ttaatagggt acatgaaggc cgacatagcg gagtaaggct tgcgaggagc
     3121 cagcgaagtc tcgcaagaag gcatccaccg cctcacgtaa ggccgacaag tcat
//
LOCUS       DASZAK010001011         5548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_417157, whole genome shotgun sequence.
ACCESSION   DASZAK010001011 DASZAK010000000
VERSION     DASZAK010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417157"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..485
                     /locus_tag="VGT44_17820"
     CDS             75..485
                     /locus_tag="VGT44_17820"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2582721.1"
                     /translation="MDTYDVAEIASILDAWYARLGRQFGPLSRPQRRVLRQLRDGASL
                     RVGEMADQLGLTTAGTTRMLDKLEEMGYITRARSSSSDQRQVYVSLSPAGEQALLASD
                     AIFLERVQLSLSGLTVTERVILEQLLRKLNETAP"
     gene            549..1001
                     /locus_tag="VGT44_17825"
     CDS             549..1001
                     /locus_tag="VGT44_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2582722.1"
                     /translation="MSNTPDISPAEYRALAEFRYELRRFLHFSEEAARAAGLEPQQHQ
                     LLLAIKGLPEEKTASVGELAERLQLQHHSTVELVNRMAVRGLIERRRDEQDQRRVLVS
                     LTAYGEEVLKKLSLLHRMELRNTGPSLVEVLNALTNGAVETDKSTILE"
     gene            1007..2935
                     /locus_tag="VGT44_17830"
     CDS             1007..2935
                     /locus_tag="VGT44_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV2582723.1"
                     /translation="MIFSFSSLWRKIANAPRSLGQLGNHEALGKLGDFSATPHLIVIS
                     GLAVAIGVIGAFVALVLLRLIGLFTNLFFFQHWGTALVSPAGNHLGIFVILVPVLGAL
                     LIGFMARYGSERIRGHGIPEAIEAILINGSKIQPKVALLKPLSSAISIGSGGPFGAEG
                     PIIMTGGAFGSMIAQFFHLTSVERKTLLVAGAAAGMSATFASPVAAVLLAVELLLFEW
                     KPRSMIPVAIASATAAVMRRYLLGTGPLFPTVAHPLFIGPGNILACALVGLLAGALSA
                     LLTIAVYASEDAFRKLPIHWMWWPAIGGLVIGAGGLIYPQALGVGYDIINQLLQGHYT
                     LQLLLGVLIVKSIIWSVSLGSGTSGGVLAPLLIMGGALGGLESLFLPNLGPGFWPLVS
                     MGAILGGTMRSPFTGIIFMLELTHDVNMLLPLLIAVVIAHGFTVLVMRRSILTEKVSR
                     RGYHLSREYATDPLEIIFVREVMRTNVVALPDVIEQRDLENILHGSFEASGQRGTASQ
                     GRKGSHRQRLFPVLDADQNMVGVMTYNDLMQLDGFDHSHTPHGQHDHAGPLQLRPMQP
                     VVAYPDEPLRAVVYRMAETGYTRFPVVERDDPDHLAGMISLNDLLKARTRNLNEERHR
                     ERVLHLRLLFPQSTNARE"
     gene            3106..4323
                     /locus_tag="VGT44_17835"
     CDS             3106..4323
                     /locus_tag="VGT44_17835"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV2582724.1"
                     /translation="MSSRAVASEYQQLIRDNVLSNQDLIKATFSGQHRDRTFPWMKVI
                     VRPVLVRGQKHMQFSYFDAKKDITKNYLGDEIAEKLEQILAAEFKNVHVQTAQNTIDI
                     RLTQKGKAFIHSTTTAGQPIEVNLTHDRQKSALLTANDAGSFLKAVGIMTQDGKIKAE
                     MQSKFRQINEFLRLVEQTGELERFNKAPLTVVDCGCGNAYLTFAFYHYLNDVLKIPTH
                     LTGIDVNGELLTRHTEKSRNLDWPGLTFQTTRIIDFQPVTPPDIVLALHACDTATDEA
                     LAQGIRWQSKMIISAPCCQHQLQEQLEHQPTPRPFEAVVRHGILKERLGDILTDSFRA
                     LILRIMGYQTEVVQFVSSEHTAKNLMIRAVKSLKVGDAKFVQEYNDLKDFWKVTPYLE
                     QLLGEDFTAIISK"
     gene            complement(4544..>5548)
                     /locus_tag="VGT44_17840"
     CDS             complement(4544..>5548)
                     /locus_tag="VGT44_17840"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582725.1"
                     /translation="SNGRAKYTSLVFIMSSLGAFVGIWLGLWLTTPAANFPLGLPFAQ
                     ATVQVVGGLPQFAGNGWRIMYGIGAALALVGIFLRFRLPESPRWLISKGRVEEADTIV
                     TDMEQRAMRKLVNLPPVGSEIHFHSGEKNATYMEVLGNPVYLRRTLLLFSMWFVAFIT
                     VYTIGVGLTTVLAGLGFPPPEAGMVAAVGVFGFIGVAVFDYYAGEALERKYWLPVSAV
                     LTIIGGVIIGLSGSGNFWVTAIGSIILFIGFNLFVPMAWAWSAENYPTRARATGFALV
                     DGIGHIGGGVGILLIAGNISTLGTVGTFLVIGGCLVVAAVIAQFGTATRQLRLDEVSP
                     "
BASE COUNT         1243 a         1537 c         1588 g         1180 t
ORIGIN      
        1 ctggaatttc aatatgctgt ctgatatact atatacatta ttaactaagt tagtcaattg
       61 tatggagcgg aaagatggac acgtacgatg tcgcggagat tgcctcgatt ctggatgcct
      121 ggtatgcgcg cctggggcgg cagtttggtc cgctctcgag gccacagcgc cgggttctgc
      181 gccaactgcg tgatggagca tcactgaggg taggcgaaat ggccgaccag cttgggctga
      241 ccacagctgg aaccaccagg atgctcgaca agctagagga aatgggctac attacacgcg
      301 cgcgctccag cagcagcgac cagcgccagg tctacgtttc gctctctcct gctggcgagc
      361 aggcactgct cgcctcggac gccattttcc ttgagcgcgt tcagctttcc ctgagcgggt
      421 tgaccgtgac ggagcgagtc atccttgaac aattgctgcg caagctcaac gagaccgcgc
      481 cttgacaggc tctagcgttc tctcgtataa taatatcgtt acacgatata ttatgcaaag
      541 aggagaacat gagcaacacc cctgatattt cgccagccga ataccgggca ttagccgagt
      601 tccggtacga gttacggcgg ttcttacatt tcagcgaaga ggcggcgcgc gccgccgggt
      661 tggaacccca gcagcatcag cttttactgg ccatcaaggg actgccggaa gagaaaacgg
      721 ccagtgtggg cgaactggcc gaacgattac agttacagca tcacagcacg gttgagctgg
      781 tgaaccgaat ggcggtacga ggattgatcg agcgtcgccg cgatgaacag gaccagcggc
      841 gcgtactggt cagtctgacc gcgtatggag aagaggtcct caagaagctt tcattgctgc
      901 atcgcatgga actacggaac accggtccct ctcttgtcga agtgttaaac gcgctcacga
      961 atggcgcagt tgagacagat aaaagcacta tactggagtg agcagcgtga tcttttcgtt
     1021 ttccagtcta tggcgcaaga ttgcaaacgc tccccgcagt cttggccagt tgggcaatca
     1081 tgaagcattg gggaagcttg gcgacttttc cgcgacaccg cacctgatcg ttatttcggg
     1141 cctcgcggta gcgatcggcg tgattggcgc ttttgtagca ttggtgttgc tgcgcctgat
     1201 cgggttattt accaatctct tcttcttcca acattgggga acggcgcttg tctcgccagc
     1261 cgggaaccac ctgggcatct ttgtaatcct ggtgcctgtg ctgggcgcgc ttctgatcgg
     1321 tttcatggcc cgctatggtt cggagcgtat tcgcgggcat ggcatccctg aagcgattga
     1381 ggccattctg attaacggca gcaagattca accgaaagtg gcgctgctca agccgctctc
     1441 atcggcgatc tcgattggct caggcgggcc atttggcgcg gaagggccga tcatcatgac
     1501 gggtggcgcg ttcggctcga tgatcgcgca attcttccac ctgaccagcg tagagcgcaa
     1561 aacgctgctc gtggcgggcg cggctgccgg gatgtcggcg accttcgcat caccggttgc
     1621 ggcggtgttg ttggccgttg agctattgct attcgagtgg aagccgcgca gcatgattcc
     1681 agtcgcgatc gccagcgcaa cagccgccgt gatgcgccgc tatctgcttg gcacgggacc
     1741 gctcttccca accgtagcgc atccactctt catcggccct ggtaatatcc tggcctgcgc
     1801 gctggtagga ctgctggcag gcgcgctttc cgcgctgctc acaatagcgg tgtatgccag
     1861 cgaggatgcc ttccgtaaat tgccgataca ctggatgtgg tggccggcca tcggtgggct
     1921 ggtgatcggc gccggcgggt tgatctatcc ccaggcgctc ggtgtgggct atgatatcat
     1981 caatcaattg ctgcaaggcc actacaccct acaactcttg ctcggcgtgc tgatcgtcaa
     2041 atcgatcatc tggtcggtat cgcttgggtc ggggacatct ggcggcgtcc tggccccgct
     2101 attgatcatg ggtggcgcgc tgggcgggct ggaatcgctt ttcctgccca atctcggccc
     2161 cggcttctgg ccacttgtca gcatgggcgc gatcctgggc ggcacaatgc gctcgccctt
     2221 taccgggatc atcttcatgc tggaactgac ccatgatgtg aatatgctcc tgcccctgct
     2281 gatcgcggtc gtgatcgcgc acggcttcac ggtgctggtg atgcgccgct cgattcttac
     2341 cgaaaaagtc agcaggcgcg gctatcacct cagtcgcgaa tacgccaccg atccactgga
     2401 gatcatcttt gtgcgcgagg tgatgcgtac caatgttgtc gccctacccg acgttatcga
     2461 acagcgagat cttgagaaca ttttgcacgg ctcttttgaa gctagcggcc aaaggggtac
     2521 agccagtcaa ggcaggaagg gcagccacag gcagcggctc tttcctgtac tggatgccga
     2581 tcagaacatg gttggcgtga tgacctacaa cgacctgatg caactggacg gcttcgatca
     2641 ttcacacact ccacatgggc agcatgacca tgctggtcct ctgcaactgc gccctatgca
     2701 gccggttgtg gcctatccgg atgaaccact gcgcgccgtc gtttatcgca tggccgaaac
     2761 cggttatact cgctttcccg ttgttgaacg cgacgaccct gaccatctcg ccggaatgat
     2821 ctcgctcaat gacctgctca aagccaggac gcgcaacctc aacgaagaac ggcatcgcga
     2881 gcgtgttcta catctacgtc tgctcttccc ccaatccacg aatgcgcgcg agtagcagga
     2941 cgtagcctga ctcttgacag tcgaactcgc ctttgggttc gagcaactga caaagctggc
     3001 gacgacgcaa gaggagcaga tggatcgcaa gcagcttgag gcgctggttg gctgattgtg
     3061 ctagaatgtg tatgaatcat acatacttaa aggggggaag aggaaatgag cagtagagcg
     3121 gtagcgagcg aatatcaaca gcttatcagg gataatgtcc tgagcaatca ggatttgatc
     3181 aaggcgactt ttagcggcca gcaccgggac cggacttttc cgtggatgaa agtgattgtc
     3241 cgtcctgtgc tggtgcgcgg gcagaagcat atgcagtttt cgtactttga cgcgaagaag
     3301 gacattacga aaaactatct gggcgatgaa atcgcggaaa agcttgaaca aatcctggct
     3361 gctgagttta aaaatgtgca tgtgcagact gcccagaaca ccatcgatat caggctcaca
     3421 caaaagggga aggcttttat ccatagcact acaaccgctg gccagccaat tgaagtgaat
     3481 ctcacgcatg atcgccagaa gagcgcacta ttgacggcca atgatgctgg ttctttcctg
     3541 aaagcggtcg gtatcatgac tcaagatggc aagatcaaag cggagatgca gagtaagttc
     3601 cggcagatca atgagttttt acgactcgtc gagcaaactg gagaactgga acggttcaat
     3661 aaagcgccgc ttacggttgt cgattgcggc tgtggtaacg cttacctgac gttcgcgttc
     3721 tatcattatc tgaacgatgt gctgaagata ccaactcatt tgaccggcat tgatgtcaac
     3781 ggtgagcttt taacgaggca caccgaaaaa agtaggaacc tggactggcc cggcctcacc
     3841 tttcagacga ccagaattat cgattttcag ccggtgacac cgccagatat tgtgctggca
     3901 ctccatgcct gcgatacggc taccgacgaa gcgttggctc agggaatcag gtggcaaagt
     3961 aaaatgatta ttagcgcgcc atgttgccag catcaactcc aggaacaact tgagcatcaa
     4021 cctactcccc ggccattcga ggcggtagtg cgtcatggta tcctgaaaga gcgcctggga
     4081 gatattttga ctgatagctt ccgcgcgttg atactgcgca tcatggggta tcagaccgag
     4141 gtagtccagt tcgtctcaag cgagcataca gcgaaaaact tgatgatacg ggctgtgaaa
     4201 tccctgaaag tgggggacgc gaagttcgtt caggaatata acgatctcaa agatttctgg
     4261 aaggtcacgc cctatctcga acagcttttg ggggaagact tcactgcgat aataagcaaa
     4321 taatcagcgg agtatacccg taggacctcc catgtcatgc tgagcgcagc gaagcatctg
     4381 gctcgatggt cgagatgctt cgctgcgctc agcatgacat taccggcttc ggttgtgaaa
     4441 attcatgatc gtccccgatg ggcgttaaag gctggcatcg gggacgatca atcggcggtg
     4501 tgcgcggtga atcggcacct acgttccata cttgacaaag atgctacggg ctgacctcgt
     4561 cgaggcgcaa ctgcctggtc gcggtgccga actgggcgat gactgccgct accacgaggc
     4621 agccgccgat taccaggaag gtgccgacgg tacccagtgt cgaaatgttg cctgcgatca
     4681 gcagaatgcc cacgcctcca ccaatatgcc cgatgccgtc caccagcgcg aagccagtgg
     4741 cgcgcgcccg cgtcggatag ttctccgccg accaggccca ggccatcggt acaaacaggt
     4801 tgaagccgat gaacagaatg atcgagccga tggccgtcac ccagaaattg cccgagccac
     4861 tcagcccaat gattaccccg ccgatgatgg tcagtactgc cgacactggc agccagtatt
     4921 tgcgttccag cgcctcgcca gcgtagtagt cgaatacggc taccccgatg aagccaaaca
     4981 cgcccactgc cgcgaccatc ccggcttccg gcggcggaaa gcccaggccc gccagcacgg
     5041 tcgtcaggcc cacgccaatg gtgtagaccg tgatgaaggc gacgaaccac atgctgaaca
     5101 gcaagagcgt ccgccgcagg tagacggggt tgcctaggac ctccatgtag gtcgcgtttt
     5161 tctcgcccga gtggaaatga atttccgagc cgaccggagg cagattcacc agcttcctca
     5221 tcgcccgctg ctccatatcg gtcacgattg tgtcggcttc ctcgacacgt cccttggaga
     5281 tcagccagcg cggcgactcc ggcaagcgga agcgcaaaaa gatgccgacg agcgccagcg
     5341 ccgcgccgat gccgtacatg atacgccagc cattaccagc aaattgaggt agcccgccga
     5401 cgacctgcac cgtcgcctga gcaaagggca gaccgagagg gaaattggct gcaggagttg
     5461 tcagccacaa gcccaaccag atgcccacga aggcgcccag cgacgacatg ataaagacga
     5521 gcgaggtata tttggcgcgc ccattgct
//
LOCUS       DASZAK010001012         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_595006, whole genome shotgun sequence.
ACCESSION   DASZAK010001012 DASZAK010000000
VERSION     DASZAK010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595006"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1791
                     /locus_tag="VGT44_17845"
     CDS             145..1791
                     /locus_tag="VGT44_17845"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV2582726.1"
                     /translation="MINWVMLSVFIVVFTSVTIMAFMAARWRPGDLTRLQEWGLAGRR
                     FGTIVSWFLLGGDVYTAYSFVAVPGLVFASGALGFYAVPYLIVVYPIVFIVLPKFWTV
                     ARHRGYVTPADFVRDRFDSGSLALLVAITGILATMPYIALQIYGIEVVIAQMGVPIEI
                     SLIIAFTILAAYTYTSGLRAPAMIALVKDACIWLVMLVAIIYIPAKLGGFGAIFNAVH
                     QKALSNPTTFHDVLPSSEYPVYATLALGSALALFLYPHTLTGILSTNSRKVVRRNAAM
                     LPAYTVLIGLICLLGYVAIAAGVELSPVYKTNSALPALFAKEFPDWFAGFSFAAIAIG
                     ALTPAAIMSIAAANLFTRNIYREYFRPTCTEREESNTARTASLVVKLGALAFILFFPT
                     TYAISLQQLSNIWIIQTLPAVFLGLYTSWFHRRALMIGLLGGLVAGTWMIVAKQFTST
                     VFSVTIGSFTIPMYSAVLALIVNLLLSVTLTPLFRMLGFSTGRDDTIPTDFEAHPVPG
                     SSESLLISQMGRNQNQNGYTATPATPPPTPSWQDRERIMR"
BASE COUNT          332 a          602 c          509 g          467 t
ORIGIN      
        1 tcggcgcgac aacacaggag ttcgcggatg tcgatatcaa tgccctgcac aatattttga
       61 tcgatgcgtt cactgctcaa caattgggca ttttcctggc gcggtagcgc gcccctacgc
      121 gacgcgtaga aagaggggaa gagtatgatt aactgggtga tgctaagcgt tttcatcgtt
      181 gtcttcactt ccgtgacgat tatggcgttt atggcggctc gctggcgccc tggtgatctc
      241 acgcgcctcc aggaatgggg attggcggga cggcgcttcg gcactatcgt ttcgtggttc
      301 ctgctcggcg gcgatgtcta taccgcatat tcttttgtgg cagtaccggg actggtcttt
      361 gcctcaggcg cgctgggatt ctatgctgtt ccctatctga ttgtcgtcta tccgatcgtc
      421 ttcatcgttc tacccaagtt ctggacagtg gcgcgccatc ggggctacgt cacgcctgcc
      481 gacttcgtgc gagaccggtt cgatagcggc agcctggctc tgctggtggc catcaccggc
      541 atccttgcaa ccatgcccta tatcgcgctg cagatctacg gcatcgaagt cgtcatcgcg
      601 cagatgggcg tccccatcga gatttcactg attatcgcct tcactatcct ggcggcctat
      661 acctacacca gcggcctgcg cgcccctgcc atgattgctc tggtgaagga cgcctgcatc
      721 tggttggtga tgctggtcgc aattatctac atcccggcca aactgggcgg cttcggcgcg
      781 atcttcaacg cggtgcatca aaaagcgctg agtaatccga cgaccttcca cgatgtgctg
      841 ccttcttctg aatatcctgt ctacgcgacg ctggcgctcg gctccgccct ggcgctcttc
      901 ctgtacccgc acacactgac gggtattctc agcacaaaca gccgtaaagt tgtgcggcgc
      961 aatgctgcca tgctgcctgc ctacactgtc ctgatcggcc tgatctgcct gctcggttac
     1021 gttgcgatcg cggccggcgt ggaactctcg ccggtctata aaacgaacag cgcgctgccc
     1081 gcgctctttg caaaagagtt cccggactgg ttcgctggct tctccttcgc cgccatcgcc
     1141 attggcgcgc tgacgcccgc tgccatcatg tcgattgccg ccgcgaacct cttcacgcgc
     1201 aacatttacc gcgagtattt ccgcccgacc tgcacggagc gcgaagagtc gaataccgcg
     1261 cgcactgcct cgctggtggt gaagttgggc gcgctggcct ttatcctgtt cttccctacc
     1321 acctatgcca tcagcctgca acagcttagc aatatctgga tcattcagac gttgccggcc
     1381 gtcttcctcg gcctatatac cagctggttc catcgccgcg ccttgatgat cggcctgctg
     1441 ggtggtctcg tggccggaac ctggatgatt gtcgcgaaac agttcacgtc aaccgtcttc
     1501 tcggtgacga taggctcgtt caccattcca atgtatagcg ccgtgcttgc gctgatcgtc
     1561 aacctgctcc tgtccgtcac gctgacgccg ctcttccgca tgttgggctt ctctacgggt
     1621 cgcgacgata cgatccccac tgacttcgag gcgcacccgg ttcccggctc gtccgaatcc
     1681 ctcttgatct cgcagatggg tcggaaccag aaccagaacg ggtacacagc cacaccggcg
     1741 acgccaccac ccacaccttc ctggcaggac cgcgagcgca tcatgcggtg atgcgctccg
     1801 gcctccctat gtcaatctac gatcctctac cgttcattta ctacaaatgc gcatgatgtg
     1861 ttttgttcac aagctcatac tatagaatga gagtggctgg ttgcgctggt
//
LOCUS       DASZAK010001013         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_26262, whole genome shotgun sequence.
ACCESSION   DASZAK010001013 DASZAK010000000
VERSION     DASZAK010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26262"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..665)
                     /locus_tag="VGT44_17850"
     CDS             complement(18..665)
                     /locus_tag="VGT44_17850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582727.1"
                     /translation="MQESDQQYPLKQPRHAFDGFKGDESDREAQIEPVEAPRTPRSQQ
                     APAHQREEPERPQLPRGHLKTALLLALLAGLIASAQTIIVTLINGGFYRQAAQFASNP
                     SKLPIGTALTIFGLFILTSVIGAVIYFIAGFVTGKIAVSRRLGFLCGFVAGAFAQLVG
                     FIVQYVPGYPASVNIGVSGGTITALILLLIVALVAGLIGFLGSWLATRRHPYYAA"
     gene            complement(674..1222)
                     /gene="lepB"
                     /locus_tag="VGT44_17855"
     CDS             complement(674..1222)
                     /gene="lepB"
                     /locus_tag="VGT44_17855"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV2582728.1"
                     /translation="MKHSHLAREIIETLALTLIIFLVIHFTVQNYQVDGTSMESSLFS
                     NEYVLVNKLAYLFHPPERGDVIVFRFPLDTTKNLVKRVIGLPGDVIQVTISTVEVNGV
                     VLHEPYVKTSVNPTGNKWIVPQGDYFVMGDNRQASYDSRDWGFVPKDYIIGKAVMVYW
                     PLNSWQFINTYSTVYSAIKASP"
     gene            complement(1246..1797)
                     /gene="lepB"
                     /locus_tag="VGT44_17860"
     CDS             complement(1246..1797)
                     /gene="lepB"
                     /locus_tag="VGT44_17860"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV2582729.1"
                     /translation="MSEKHARLVREVVETIAVTLLIFIVIRFAVQSFRVDGPSMQPGL
                     HTDDYVLVNKAAYLFSGPQRGDVIVFRYPLDTSKDLIKRIVGLPGDIVQTTSTDVIVD
                     GQILHEPYISMPFNFESHAWKLGPNQFFVMGDNRDNSLDSRTWGPLDKSYIIGKAVAV
                     YWPFGDWNVINTFSSVFVAVRPQ"
     gene            1932..4259
                     /locus_tag="VGT44_17865"
     CDS             1932..4259
                     /locus_tag="VGT44_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582730.1"
                     /translation="MRLPARLFRQRALLLLTLLLLLALLAWRALPASITLPANAAPRR
                     LAPLVGTLTGGGTWHGAQDDLLYVVAFSSAQQPYGPLVVYRAFGTDLSAALPILIVPR
                     SPLNLTPVLYPSPDGRALALLNPLSTGYNNTINGASLSIVSTYGTGSTMLAQHVALAD
                     PPIWTSDSRAVYYMTGDEINQEIHRVDLSGHDTMLYRQSLAQGSMRLVGLDRSGALII
                     TLARPNMPVTIERLNAGHATTPTPVITLPPDILPGNVLRVGSDGASLVYTRATGAPFQ
                     VAFATHIITIAAPLFDSSRYDHNLVPLAISSNGTILAMSQVVSIRSDLAAQGIANMPE
                     QERLLLVNASGSAWQALQLPDGGQLLQAFWTGHVNPAKVHAASLPGRQRASNGPEQNG
                     TVYQQDLWMLEGHANQLFDGPKLPTMCYGLCSSPNGAPHISAAILHGVAYTESDWHQF
                     NSSDFHVNGEQVGSPLESFDGGWGEYQQTWGMPPQCKAANNCRSDVYRIEHDQAYNIG
                     VGIQSLIHAWNSTAGVVSSSDPNDPYKANDWFFAVWAYNGSYGNNPNDVPSSQYGHWY
                     PGAPFNSIYEERVWYFAAHPQSASTGWTDNYVPSLGPALLPPQADFVKTSDSFVDCVT
                     CSIPDWTSGSYDREWVGYGAPNAQAASSFTTAFAQLGGENALGLPRDNGGGAAAHRWG
                     SGLAQDFGGGSDQPGTLLLADNTTTAYFVYGSLWTQYLVADHGAPGCHGYPTSNLFPF
                     SDPGLGSDTYLRQTFQQGYMIWDATIGVIIADICL"
BASE COUNT          916 a         1333 c         1179 g          843 t
ORIGIN      
        1 gggcatggcc gcatgtctta cgcggcgtaa tacggatgcc tgcgcgtggc aagccaggag
       61 ccgaggaagc cgatcagccc ggcgaccagc gccacgatca gcagtaagat cagcgcagta
      121 atcgtgccac cgctcacgcc gatattgacc gaggcgggat agccgggaac atattgcacg
      181 atgaagccga ccagttgcgc gaaagcaccc gcgacgaagc cgcacaggaa acccaaccga
      241 cggctcaccg cgattttacc agtgacgaag cccgcgatga agtagatcac cgcgccgata
      301 accgaggtca ggatgaacag gccgaaaatg gtgagggcgg tgccaatcgg cagtttagag
      361 ggattggaag cgaactgggc agcctggcgg tagaatccac cattgattag cgtgacgatg
      421 atcgtctgag cggaggcgat cagtccggcg agcagcgcaa gcagcagcgc agttttcaga
      481 tgtccgcgcg gcaactgcgg gcgttccggc tcttcacgct ggtgcgcagg agcctgctgg
      541 gaccggggag tccggggagc ctcgaccggc tctatctggg cctctctatc gctttcgtcg
      601 cctttgaaac catcaaaggc atgccgcggc tgtttgagcg ggtattgctg gtctgactct
      661 tgcatcttct ctcctatgga ctggccttaa tcgcgctgta caccgttgaa taggtgttga
      721 taaattgcca gctatttaac ggccagtaaa ccatgaccgc cttcccaatt atataatctt
      781 ttggcacgaa tccccaatca cgcgagtcgt agctggcctg gcgattgtcg cccatcacaa
      841 agtagtctcc ctgcggcaca atccatttgt tgccggtcgg gttcaccgag gtcttgacat
      901 acggctcgtg aagcaccacg ccattgacct cgacggtaga aatcgtgacc tgaatgacat
      961 cgcccggcaa gccaatcacg cgcttgacga ggttcttcgt cgtatcgagt ggaaaccgga
     1021 acacaatcac atccccacgt tcggggggat ggaacaggta cgcgagcttg ttcaccagca
     1081 catattcgtt gctgaacagg ctggattcca tgctggttcc atcaacctgg taattctgaa
     1141 cggtgaagtg gatcaccagg aatataatga gtgtcagcgc gagggtttca ataatttccc
     1201 gcgccaggtg cgaatgtttc acaggcaaac ctcgtttatt cgttgctatt gggggcgaac
     1261 ggccacgaag accgacgaga aggtgttaat cacattccag tcgccaaacg gccagtagac
     1321 tgcaacggcc ttcccgataa tatagctttt atcgagtggc ccccaggtgc gcgagtcgag
     1381 actgttatct cgattatctc ccatgacaaa gaactgattc ggccccaact tccacgcatg
     1441 gctctcaaag ttaaacggca tactgatata cggttcgtgt aaaatctgcc catccacaat
     1501 gacatccgta ctagtcgtct ggacgatgtc gcccggcagc cccacaattc gcttgatgag
     1561 atctttgctg gtatccaatg gatagcgaaa aacgatcaca tctccgcgct gcggtcccga
     1621 aaacaggtac gccgccttgt tgaccagcac ataatcatcc gtatgcaaac ccggctgcat
     1681 actcggccca tccacgcgaa agctctgcac ggcaaaacga atcacgataa agatgagtaa
     1741 cgtcacggca atggtttcca ccacttcccg gacgagacgg gcatgttttt cagacaccga
     1801 gctatccctc ttgcttgcgc ttcctgcgat tttcattgtt gctcattata gcgacatcac
     1861 accaccaggg caacaacccc attactatga cttttgtctc atctgtatgc tggtaataga
     1921 ggaacatgag tatgcgttta cctgcacgac tatttcggca gcgggcattg ctgctgttaa
     1981 cattattgct actgctggcc ttactcgcct ggcgcgcatt accggcatct attaccttgc
     2041 cggcaaatgc cgcgcctcgt cgcctggctc ccctcgtcgg cacactgacc ggcgggggca
     2101 cctggcacgg cgcccaggac gatttgcttt acgtcgtcgc gttcagcagc gcgcagcagc
     2161 catatggccc gctggtcgtt tatcgcgcct tcggaaccga cctgagcgcc gccctgccga
     2221 ttctgattgt gccgcgcagc ccgcttaacc tgacgcccgt actctatcct tcacccgacg
     2281 ggcgcgccct cgccctgttg aacccgcttt cgaccggcta taacaatact atcaacggcg
     2341 catccctcag catcgtctca acctatggaa caggctcgac catgcttgcg cagcatgtcg
     2401 ccctggccga cccgccgatc tggacatctg atagtcgcgc cgtgtattac atgacgggcg
     2461 atgaaatcaa ccaggagatc caccgcgtcg atctctccgg ccacgacacc atgctctatc
     2521 gccaatcact ggcgcagggt tcgatgcgcc tggtcgggct ggatcgcagc ggcgcgctga
     2581 tcatcacgct ggcacggccc aatatgcccg tcacaatcga acgcctgaac gccggacatg
     2641 ccaccacacc aacgcccgtg attacgctgc cgcccgacat tctgccgggg aatgtgctgc
     2701 gcgttggcag cgatggcgcc tccctggtct acacgcgagc caccggcgcg ccattccagg
     2761 tcgcctttgc cacccacatc atcaccatag ccgcgccgtt gttcgacagc agccgctatg
     2821 atcacaacct cgtgccgctc gccatctctt ccaacggcac aatcctggca atgtcgcagg
     2881 tcgtgagcat acggagcgac ctggcggccc aggggatcgc caatatgcca gagcaggagc
     2941 ggcttctgct ggtcaacgcg agcggcagcg cgtggcaggc gctgcaacta ccagacggcg
     3001 gccagctgct acaagcattc tggacggggc atgtcaatcc agcgaaagtc catgcagcgt
     3061 cgttgccggg gcgacagcgc gcgagcaacg ggccggaaca aaatggaacc gtttaccagc
     3121 aggacctgtg gatgcttgaa ggacacgcca accagctttt cgatgggccc aaactgccca
     3181 cgatgtgtta cgggctatgc tcatcgccca atggcgcgcc gcatatctcg gcagccatcc
     3241 tgcacggtgt ggcctatacc gaaagcgact ggcaccagtt caatagcagc gatttccatg
     3301 tcaacggcga gcaagtggga tcgccgttgg aaagcttcga tggcggctgg ggcgaatacc
     3361 agcagacgtg ggggatgccg ccgcagtgca aggccgctaa taattgccgc agcgatgtct
     3421 accgcatcga acacgaccag gcgtacaaca tcggcgtggg cattcaatcg ctcatccatg
     3481 cctggaacag cacagccggt gtggtttcga gcagcgatcc aaacgacccc tacaaagcca
     3541 acgactggtt tttcgccgtg tgggcctaca acggctccta tggcaacaac ccgaacgacg
     3601 taccatcctc gcagtacggc cactggtatc cgggcgcgcc cttcaactcc atttacgaag
     3661 agcgcgtctg gtatttcgcg gcccacccgc agtctgccag caccggctgg acggataact
     3721 atgtaccgag cctggggcca gcgcttctgc cgccgcaagc agatttcgtc aaaacatccg
     3781 actcgttcgt tgactgtgtg acctgctcaa tccccgactg gacgagcggc agctatgatc
     3841 gcgagtgggt cggctacggc gcgccgaatg cccaggcagc cagcagcttt acgacggcct
     3901 tcgcgcagct tggcggcgaa aacgcgctgg gcttgccgag agataacggt ggcggcgcgg
     3961 cggcgcatcg ctggggcagc ggcctcgcgc aggatttcgg cggcggctcc gaccagccgg
     4021 gaacgctgct gctggccgat aacaccacga ccgcctactt cgtctacggc agcctctgga
     4081 cacaatacct ggtggccgat cacggcgcgc ccggctgtca cggctatccc acctcgaacc
     4141 tgttcccctt ctccgacccc ggcctcggca gcgacaccta cctgcgccag accttccagc
     4201 agggatacat gatctgggat gccaccatag gagtcatcat cgccgacatc tgcctgtagg
     4261 gacagggggc g
//
LOCUS       DASZAK010001014         2072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_701456, whole genome shotgun sequence.
ACCESSION   DASZAK010001014 DASZAK010000000
VERSION     DASZAK010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701456"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VGT44_17870"
     CDS             <1..741
                     /locus_tag="VGT44_17870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582731.1"
                     /translation="QPSISVNCGSPVISRVSIPDGVLDRANQHSTEHTRDIPWRRNAA
                     VKTLPGSLASTWGTTAAERTRSFPCDRYLSPFDAAYFRAVPVQAPAPIVFRWLCQLRV
                     APYSYDWIDNGGHKSPRELIPGLDQLQVGQRVMTIFQLVDYEPNRHLTLRLRVARAKA
                     LFGEIACSYVILPREDAWCRLVVKLLVRYPPGYTGAMMRRWLPWGDLFMMRKQLLTLK
                     HLAERQSRDAKKEEKHARDRIVGRDHRI"
     gene            701..1534
                     /locus_tag="VGT44_17875"
     CDS             701..1534
                     /locus_tag="VGT44_17875"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2582732.1"
                     /translation="MQEIALSAGIIEYEDTGGSGPVVVLLHGLVMDGSLWRHVVGELR
                     SDHRCVVPTLPLGSHRQPMRADADLSPHGIARLQAGFLEALDLHDVTLVGNDLGTFQI
                     TAGWYPERIARLVLTSCEAFENFPPGLPGRAAEVGIKMPGGAFLMAQVLRLRALRRLP
                     FTFGWMTKRPIPNEILDAWFRPLQTQPGVRRDLTKYVQTLDKGDMLVAAERLRSFDRP
                     ALVIWAKEDRIMPPSHGRRLAELLPHGRLIEIADSYTLIPEDQPGEMARAIRQFIRDT
                     P"
     gene            complement(1783..>2072)
                     /locus_tag="VGT44_17880"
     CDS             complement(1783..>2072)
                     /locus_tag="VGT44_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEV2582733.1"
                     /translation="YTSRAYRLTVAQSGIMVSMSRKGNCWDNAAMESFFGSLKEECVG
                     NTIYASHEEARLALFTYLEVYYNRIRRHSTLGYVSPLVYEQRTLQQAKENV"
BASE COUNT          428 a          614 c          586 g          444 t
ORIGIN      
        1 cagccctcca tctcggtgaa ctgcggctca ccagtgatct cgcgcgtcag catacctgat
       61 ggggtactcg acagggctaa tcagcacagc acagagcata ccagagacat accctggagg
      121 agaaacgccg ctgtgaaaac actacccgga tctcttgctt ccacctgggg aacgactgcc
      181 gccgagagaa cacggtcgtt tccctgcgat cgctatctca gccctttcga tgcggcctat
      241 ttccgtgctg tgccggtaca ggcgccagcc cccatcgtct ttcgctggct ctgtcaattg
      301 cgcgtggcgc cctacagcta cgactggatt gataacggtg gtcacaagag ccctcgcgaa
      361 ctcatcccag ggctggacca gctacaagtt gggcagcgcg tgatgacaat ttttcagttg
      421 gtagactacg aaccgaaccg acacctcacg ctgcgcctaa gggtggcgcg tgccaaagct
      481 ctctttggtg agatagcatg cagctacgtt atcctcccac gggaagacgc gtggtgccgt
      541 ctcgtggtca agctgctcgt tcgctatcct cccgggtaca caggggccat gatgcgccga
      601 tggttaccat ggggagactt gttcatgatg cgcaagcaat tgttgacact caaacacctg
      661 gccgaacgcc agtccaggga cgctaagaag gaggagaaac atgcaagaga tcgcattgtc
      721 ggcagggatc atcgaatatg aggatacagg cggcagcggc cctgtggtgg tcctgctgca
      781 cggattggtc atggacggct cgctgtggcg tcacgtcgtg ggcgagttgc gcagcgatca
      841 ccgctgtgtg gtcccgacac tgccccttgg cagccaccgg cagccgatgc gagcggacgc
      901 tgatctctca ccacacggca tcgcgcgact tcaggccggg tttctggaag ccctggacct
      961 gcacgatgtg accctggtag gcaacgacct gggcacgttt cagatcaccg ctggctggta
     1021 tcctgagcgt atcgcgcgac tcgtgctcac ttcctgcgaa gcttttgaga acttcccacc
     1081 cggtctgcct gggcgggccg cagaggttgg catcaagatg ccgggtgggg ccttcctcat
     1141 ggcacaggtc ctgcgcttgc gcgcactgcg ccgcctgcca ttcacctttg gatggatgac
     1201 gaagcgaccg attcccaacg aaattctcga cgcctggttt cgccccttgc agacccagcc
     1261 gggggtccgc cgcgatctga ccaagtacgt gcagaccctc gacaaggggg acatgctcgt
     1321 tgcggctgag cgcctgcgct ccttcgaccg tccggcgctc gtcatctggg cgaaggagga
     1381 tcgcatcatg ccgccttcgc acggacggcg gctggctgaa cttcttccac acgggcggtt
     1441 gatcgagatt gccgacagct acaccctcat ccctgaagat caacccggcg aaatggctcg
     1501 tgccattcgt cagttcatcc gagacacgcc atgagtacgt ttgtccgacg tagaagcgac
     1561 aggtatggca cgagtccggc tcaaccgcgg cgcgatgatg aatgttaaca accaggatcg
     1621 gtcatacccc gtaggcggcg cgccaataaa ttgtgcgcct acgacagggt gagcttgccc
     1681 cgttttcgta gagtctctcc aggttggagc tgaggagaat gcctgacgtt tctacgtcac
     1741 gtaacgctaa tgttgttatt ctaatataag aaacgttcta cgtcaaacgt tttccttcgc
     1801 ctgttgcaga gtcctctgtt catagacaag tggactcacg tatccgagcg tcgaatggcg
     1861 gcgaatgcga ttgtaataca cctccaggta ggtgaagagc gccagacgcg cttcctcatg
     1921 agatgcatag atggtgttgc ctacacattc ttctttcaag gacccgaaaa aactctccat
     1981 cgctgcatta tcccagcaat tgcctttccg tgacatactg accatgatcc cggattgtgc
     2041 tacagtcagg cgataagctc gtgaagtata tt
//
LOCUS       DASZAK010001015         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_701458, whole genome shotgun sequence.
ACCESSION   DASZAK010001015 DASZAK010000000
VERSION     DASZAK010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701458"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..448)
                     /locus_tag="VGT44_17885"
     CDS             complement(128..448)
                     /locus_tag="VGT44_17885"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEV2582734.1"
                     /translation="MTQTDTPNDQQTPDYKSYTEELQVMGEQLLSKVKELLHEGNVRR
                     IIIKQEGQSIMEFPLTFGVVGVLVAPVLAAIGAIGALIAQCSIEVVRVERPGSGDDVP
                     PSAL"
     gene            664..1065
                     /locus_tag="VGT44_17890"
     CDS             664..1065
                     /locus_tag="VGT44_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEV2582735.1"
                     /translation="MAYFLAKTDPEHYSLADLERDGATIWDGVRSAAALLVIRAMHPG
                     DELLIYHSQGEAAIVGLARVASEPRPDPNDAKSWVVDVAFVRRLAGPVTLREIKESHR
                     FDDWALVRQSRLSTMSVPPNVVAWLQEKHVL"
     gene            1081..1929
                     /locus_tag="VGT44_17895"
     CDS             1081..1929
                     /locus_tag="VGT44_17895"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEV2582736.1"
                     /translation="MALDHEAFWYVGGQWVHPQEATLSINDIAILRGYSVFEALRTYD
                     RRPFHLEEHLRRLYHSAKIIELKIPWQPERIYNIINEAIARNSYKHAAIRLLVTGGES
                     EDGLTATGQPTLVVMITPLGERDMQRFSRGVRLITTSLQRESPEAKTTNYMAAVRSLK
                     EAERRGASDALFVNDERHVLEATRSNFFLFRGDTLVTPREGVLHGITRGVVLDLARGR
                     FPIEERAIQLDELALADEAFISSSSKEITPVVQIDDLAIGNGIPGTRTYELEQRFIEM
                     VNRGNW"
     gene            complement(1951..>2235)
                     /locus_tag="VGT44_17900"
     CDS             complement(1951..>2235)
                     /locus_tag="VGT44_17900"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2582737.1"
                     /translation="RFPLIGDVRGLGGMMAMELVKSPTTKEPDAHAASDILAAAHRRG
                     LVLIKAGMYDNVLRILVPLSVTDEQLEQGLNILEEAITVVAQAEAITTVG"
BASE COUNT          483 a          644 c          622 g          486 t
ORIGIN      
        1 cagaggccag tgttctgttt tttacgcacg gatacacaaa acagccagag caaatcagcg
       61 cgaacaccgg tttgctctgg ctacaatcgc catttacgat aaatgaacct tacactgttg
      121 gcgagtgtta gagcgccgac ggcggaacgt catcaccgga gccgggacgc tcgacgcgca
      181 caacttcgat ggaacactgc gcaatcagcg cgccaatcgc gccaatcgcg gccaacaccg
      241 gggcgaccag gacgcccacc acgccgaagg tcagcgggaa ctccatgatg gactggcctt
      301 cttgtttgat gatgatgcgg cgcacattgc cctcgtgcag tagctccttt acctttgaaa
      361 gcaactgctc gcccattacc tggagttctt cggtgtaact cttgtaatcg ggggtctgct
      421 gatcgttggg tgtatctgtc tgtgtcatag ccttctcctt tttctcttct cttgtatatt
      481 tttactggtg tgggcgcatt cgcgtcccca cccttcacac ctgtatcatg atgtgctatg
      541 ctgttcgcgt agtcgcgcct ttttgatgtt gactacatta tctatacgaa tgattcaaat
      601 gaaaagttgg aaggattctg cgatttgagc aatgcaggat cgttgtgaag aagatgaaga
      661 gctatggcct attttctcgc aaagacagac cccgaacact actccctggc cgacctggaa
      721 cgcgacggcg cgaccatctg ggacggcgtg cgcagtgccg ccgcgctgct ggttattcgc
      781 gccatgcacc ccggcgatga gttgttgatt taccatagcc agggtgaagc cgccatcgtc
      841 gggctggcgc gcgttgcatc agaacctcgc cctgacccca acgacgcgaa aagttgggtg
      901 gttgacgtcg cctttgtgcg ccgcctcgca ggacctgtca cgctgcgcga gatcaaagag
      961 agtcaccgct ttgatgattg ggcattagtg cgccagagtc gcctgtccac gatgagcgtc
     1021 cctcccaacg ttgtagcatg gctacaagaa aaacacgttc tgtagaaatc gagcgattgt
     1081 atggcactgg atcacgaggc attctggtat gtcggcggtc aatgggtaca tccccaggag
     1141 gcgacgctct cgatcaacga tatcgccatc ctgcgcggct atagcgtctt cgaggcgctg
     1201 cgcacctatg accgccgacc atttcacctg gaagaacatc tccggcggct gtatcactct
     1261 gcaaagatta tcgagctgaa gataccctgg cagcctgaaa gaatctacaa tatcatcaat
     1321 gaggcgatcg cgcgcaacag ctacaaacat gccgctattc gcctgctggt gacggggggc
     1381 gaaagcgagg acggtctgac ggcaaccggc cagccaacgc tggtcgtgat gattaccccg
     1441 ctcggtgagc gcgacatgca acgcttctcc aggggcgtca ggctgataac aacaagcctg
     1501 caacgcgagt cgccggaggc caaaacaacg aactatatgg cggcggtacg ctcgctgaaa
     1561 gaagcagagc ggcgcggcgc atcggacgcc ctctttgtca acgacgagcg tcacgtgctg
     1621 gaggccacgc gcagcaactt cttcctcttc agaggtgata cgctcgttac gccgcgcgaa
     1681 ggcgtcctgc acggcatcac acgcggcgtc gtacttgacc tggcgcgtgg ccgcttcccg
     1741 attgaagagc gcgccattca acttgatgaa ttggcattgg ccgacgaggc gtttatctcc
     1801 tcgtcttcca aagaaatcac ccccgtcgta caaatcgatg accttgcaat aggcaacggc
     1861 attccaggaa cacgcaccta cgagttggag caacgcttca tcgagatggt gaatcgcgga
     1921 aactggtaat ccacggattc gatgatgttg ttagcctacc gttgtgattg cctccgcctg
     1981 tgccacgacc gtgatcgcct cttcgaggat attcaagccc tgctcgagct gttcgtcggt
     2041 gacgctgagc ggcacaagga tgcgcagcac gttgtcgtac ataccggcct taatcagtac
     2101 cagcccgcga cgatgggcag cggccaggat atcgcttgcg gcatgggcat cgggttcttt
     2161 ggtcgtggga ctcttgacca gttccatcgc catcatgccg cccaggccgc gcacgtcgcc
     2221 gatcaggggg aaacg
//
LOCUS       DASZAK010001016         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_98592, whole genome shotgun sequence.
ACCESSION   DASZAK010001016 DASZAK010000000
VERSION     DASZAK010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98592"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..558
                     /locus_tag="VGT44_17905"
     CDS             <1..558
                     /locus_tag="VGT44_17905"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEV2582738.1"
                     /translation="MVARRWRRHAKIQAWAGDHKGSPLQTMNTINRPLQLDKAWNVVD
                     NSREGTRMERDGSEVGKPKNTMRQEFGRLGERLAAEALRERGYRILQQNFRCRYGEID
                     LVAEDALDLVFVEVKTRKGSLYGLPEEAVTRGKRRKLVQVASYYLDFHAIPERSWRID
                     VVAVQFSKAGKLEEIRVYQYAVSEE"
     gene            591..1076
                     /locus_tag="VGT44_17910"
     CDS             591..1076
                     /locus_tag="VGT44_17910"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2582739.1"
                     /translation="MSDFVESAKNIVSSAVSRTSWQAQKQLRVRAKQSEIDQLLEQRT
                     KLLDELAKIARGLYQQGRLSDAQLSRLCASIQELDHDVQNREQQLLEVKSEQYNVAQF
                     APSTTANYAPPPVSQTPPPAAPASSAAPGSARAGKVCPNCGSPVRPNALYCRTCGAKL
                     R"
     gene            1172..1723
                     /gene="tsaE"
                     /locus_tag="VGT44_17915"
     CDS             1172..1723
                     /gene="tsaE"
                     /locus_tag="VGT44_17915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV2582740.1"
                     /translation="MIDRNQEVMTATLDSISHSEAQTQRLGARLGELLRGGELILLNG
                     QLGTGKTTFTQGLAQGLGIVEQVNSPTFTLLKEYTRRSSAKQAVAPKHARPALYHFDL
                     YRLDDPAEIFDLGFDEYFEGNGVCVVEWAEKADLYWPTDHLNIRLKMLSETKRGLLFT
                     AVGSRYCELLRQFQKNTYVTTGS"
     gene            1704..2534
                     /gene="tsaB"
                     /locus_tag="VGT44_17920"
     CDS             1704..2534
                     /gene="tsaB"
                     /locus_tag="VGT44_17920"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV2582741.1"
                     /translation="MLLLALDTSTRQASIALCTEETLLGEYTWQVGNNHSVELLERIQ
                     RLVAEGGFDMSAIEAVAVATGPGSFNGLRVAVATSKALAFSLGLPLIGVSTLDIIAAH
                     QRQWRGPTCALLEAGRSELYAACYLFDAVAESHVLTHSMRRLSDYLLDSPQQLALFLL
                     EHLREWVGVPGKRLLPDVLFCGEVSEASRQALMQELPANSLFLTPLQSARHASVLAAL
                     AFERLQSGVLDDPLQLEPLYLRRPSITTSTRKQPLLGAKTHRSSELVSTEREEGALQH
                     "
     gene            2539..3150
                     /gene="rimI"
                     /locus_tag="VGT44_17925"
     CDS             2539..3150
                     /gene="rimI"
                     /locus_tag="VGT44_17925"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV2582742.1"
                     /translation="MTMADVPRVVEIERLAYPSTWPPSAYRRELQDNRWAHYIVLRDK
                     KIAEERIATPVPEPERPRRIFPLSLLPARSTATVTAPNLVSIVGFAGLWLMVDEAHIT
                     TIAMHPDFQGQGLGELMLANLIDIAYAINAKWVTLEVRVTNYRAQNLYRKYGFHNAGM
                     RHRYYSDNNEDALIMWTNEINSSAFKQSFQELRSRLMQKLEEV"
BASE COUNT          725 a          871 c          938 g          671 t
ORIGIN      
        1 atggtcgccc gccggtggag acgacacgca aagatacagg catgggcggg cgaccataaa
       61 ggctcgcccc tacagacgat gaataccata aatcggccat tacaactcga caaagcgtgg
      121 aacgtagtag ataatagtag agaaggtacg cgcatggaac gcgatggcag tgaagttgga
      181 aagccaaaga ataccatgcg ccaggaattc gggcggttgg gtgagcggct ggcggcggag
      241 gcgttgcgcg agcgcggcta tcgtattttg caacagaact tccgctgccg gtatggagag
      301 attgacctgg tggctgagga tgctctggac cttgtttttg tcgaggtgaa gacgcgcaag
      361 ggcagcctgt atggtctgcc cgaagaagcg gtgacacgcg gcaaacgccg caagctggtg
      421 caggttgctt cctactacct cgattttcat gccataccgg agcgttcctg gcgcatcgac
      481 gtggtagcgg tgcagttcag taaagcgggt aaactggaag aaatacgtgt ctaccaatat
      541 gctgtgagcg aagaataaac tatacatcgg attggaaagg gagatcatct atgtcagatt
      601 ttgtagaatc cgcaaaaaat atcgtcagtt ccgccgtcag ccgcacgagc tggcaggcgc
      661 agaaacagct gcgtgtgcgc gccaagcaga gtgaaattga tcaacttctc gaacaacgca
      721 caaagctact ggatgagctg gcaaaaatcg cgaggggttt ataccagcag ggcaggctga
      781 gtgacgcgca gttgtcgcgg ctgtgcgcca gcatacagga actcgatcac gatgtgcaaa
      841 atcgcgaaca gcagttgctg gaagtgaaaa gcgagcagta taacgtggcc cagttcgctc
      901 cgtctacaac ggccaattat gcgccgcctc ccgtttcgca gacgccgccg cccgcagccc
      961 cggcatcctc cgccgcgccg ggttcagctc gagcgggcaa ggtttgccct aattgtggca
     1021 gcccggtgcg ccccaatgcg ctgtattgcc gcacctgcgg cgcgaagctg cgctgaaatc
     1081 ctgtgtactt tcataccaac tatgctataa tgatacgtac tttaccggca gtgcatgtat
     1141 ggcggataat tgaaacgtga aggacgcgtt attgattgat cgcaaccagg aagtcatgac
     1201 ggcgacgctt gacagcatta gccacagcga agcgcagacg cagcgacttg gggcgcggct
     1261 gggtgagctt ttgcggggcg gggaactcat cttgctgaat ggacaactgg gaacgggcaa
     1321 aaccaccttt acgcaggggt tggcgcaggg cctgggtatc gtggagcagg tcaacagtcc
     1381 caccttcacg ctgttgaaag agtatacgag gcgttccagt gcaaagcagg cagttgcgcc
     1441 gaaacacgcg cgccccgcgc tttaccattt tgacctgtac cggctggacg atcctgctga
     1501 aatctttgat cttggctttg atgaatactt tgaagggaat ggcgtttgtg tcgtggagtg
     1561 ggcagagaaa gcagacctgt attggccgac cgatcatttg aacattcgcc tgaaaatgct
     1621 cagtgagacc aagcgcggcc tgctcttcac tgccgttggt tcgcgctatt gtgaactgct
     1681 gcgacagttt cagaaaaaca cctatgttac tactggctct tgatacttcc acccgccagg
     1741 ccagcatcgc actctgcacg gaagagacac tgcttggcga atatacgtgg caggttggca
     1801 acaatcatag cgtagagctg ttggaacgca ttcagcgcct tgtggctgaa ggcggttttg
     1861 acatgtccgc aatcgaggca gtcgccgtcg ccaccggacc gggatcgttc aatggcctgc
     1921 gcgttgccgt cgcgacgtcg aaggcgctgg ccttctcgct tggcctgccg ctcatcggcg
     1981 tcagcacgct ggacatcatc gctgctcacc agcggcagtg gcgcggcccc acgtgcgcgc
     2041 tgctcgaagc gggacgctcc gaactctacg cggcctgcta cctgtttgac gccgtcgctg
     2101 agtctcacgt gctgacacat tctatgcggc gactcagcga ttacctgctc gactcgcccc
     2161 aacaactcgc gctcttcctg ctagaacatc tacgggagtg ggtcggcgtt ccaggcaagc
     2221 gcctgcttcc cgatgtgctg ttctgtgggg aagtgagcga ggcatcgcgc caggcgctca
     2281 tgcaggaatt acccgcgaat agcctgtttc ttacgccgtt gcaatcggcg cgtcatgcgt
     2341 ccgttcttgc tgccctggct tttgaacgac ttcaaagcgg cgttcttgat gatccattgc
     2401 aactcgaacc cttgtacctg cggcgaccct ccatcacaac gagtacgcgc aagcaaccat
     2461 tgttaggggc aaaaacgcac cggtcgagtg agttggtgag tacagaaagg gaagaaggtg
     2521 cgttacagca ttgatcgtat gacaatggcg gatgtgccgc gcgtcgtcga gatcgagcgc
     2581 ctggcgtatc catccacctg gcctcccagc gcctatcgcc gggaactgca agataaccgc
     2641 tgggcgcatt acatcgtcct gcgtgataag aaaatcgccg aggagcgcat tgcgacgccc
     2701 gtaccggaac cggaacgtcc ccggcgcatc ttcccgcttt cgctgctgcc agcccgctct
     2761 actgcgacgg tcaccgcgcc aaacctggtc tcaatcgtcg gtttcgcggg actatggctg
     2821 atggtggacg aggcgcacat taccacgatt gccatgcatc ccgattttca aggccaggga
     2881 ttgggagaat tgatgctggc caacctgatc gatattgcct atgccatcaa cgccaagtgg
     2941 gtcacgctcg aagtgcgcgt caccaattac cgggcgcaga acctctaccg caaatatggt
     3001 tttcacaatg ccgggatgcg ccatcgctac tacagcgata ataacgagga tgccctgata
     3061 atgtggacga atgagatcaa ttcttctgcc ttcaagcaat ctttccagga actcagatcc
     3121 aggctcatgc aaaaattgga ggaagtatag tggggttggc gaaatcacat gtggttatga
     3181 agggcgaccg caagggtgtg ccctt
//
LOCUS       DASZAK010001017         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_701509, whole genome shotgun sequence.
ACCESSION   DASZAK010001017 DASZAK010000000
VERSION     DASZAK010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701509"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VGT44_17930"
     CDS             complement(<1..1068)
                     /locus_tag="VGT44_17930"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEV2582743.1"
                     /translation="MKKRIERLRGMHDLLPGAYQQQHRLIDRLSTFLAQAGYDPVDSP
                     VLEQSDLFQMSFGQELWQNLYTFRLHHRDLCLRPEYTASICRLYLDHYQRQPLPLRFQ
                     YAGPVFRYEAPGRGRYRQHTQLGIELFGGQAMAADAEILQLACDALNELHITAYKLEL
                     GHIGVASGFINRLHLDDHAARLLLSLMEQISRSADGEQMAQARLEALYPENQATGDAV
                     YSGSMSEVEANLIAPLLSGISISFGDDDARHEIVERFLWKMERGEQRRQILDALEFLR
                     MLHAISGPPPGVFDALRDLLGRYELDATPLTELEQLVTMIEQSGVPRQQMLLNLALGR
                     GVSYYTGLVFEIHAQDEEGFDA"
     gene            complement(1095..>1787)
                     /gene="aroF"
                     /locus_tag="VGT44_17935"
     CDS             complement(1095..>1787)
                     /gene="aroF"
                     /locus_tag="VGT44_17935"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV2582744.1"
                     /translation="MTTAEAVRKEGAVILRGGAFKPSTSPYGFRGMGEEGLKLLAKAR
                     GEFGLAIITEVMTPTDVEMVCEYADILQIGTRNMQNYMLLDEVGRVEKPVLLKRGMSA
                     TIEEWLLAAEYVLSQGNSRVILCERGIRTFEKTTRNTMDISAIPVIKRHSHLPIIADP
                     SQGTGHRDLVSAMSLASVAAGADGLLVEVHPNPDEALKDGAQSLTLDQFKSLMPQVQA
                     VSSAVGRKLVTE"
BASE COUNT          381 a          523 c          486 g          397 t
ORIGIN      
        1 ggcgtcaaaa ccctcttcat cctgggcgtg tatctcgaag accaatccgg tataatagct
       61 gacgccgcgc ccaagcgcca ggttaagtag catctgctgg cgtggtactc cgctctgctc
      121 gatcatcgtc acaagctgct caagttccgt tagtggcgtg gcatcaagtt catagcgtcc
      181 cagaagatcg cgtaacgcat caaagacccc cggaggtggg cccgagatag cgtgcagcat
      241 acgcaaaaac tccagcgcat ctaaaatctg cctgcgttgc tcgccgcgct ccatcttcca
      301 gagaaatcgc tccacgattt catgccgtgc atcatcgtca ccaaagctga tgctgatgcc
      361 gctcaacaaa ggcgcgatca aatttgcctc cacctctgac atcgatccgc tatacacggc
      421 atctccagtt gcttgattct ccggatacag ggcctcgaga cgagcctggg ccatttgttc
      481 accgtcggca gaacgactga tctgttccat caggctgagc agtaaacgag cggcatggtc
      541 atcgaggtgc aggcgattga tgaagccgct ggcaactccg atatgtccca gttctaattt
      601 gtaggccgta atatgcaatt cattgagtgc atcacacgcg agttgcagga tttcagcatc
      661 agcagccata gcctggccgc cgaataactc gatgccgagt tgcgtgtgct ggcgataacg
      721 accgcgcccg ggtgcttcat agcgaaagac cggcccggca tactggaagc gtagtggcag
      781 cggctgccgc tggtaatgat cgagatagag gcggcagatt gacgcggtgt attctgggcg
      841 caggcacagg tcgcggtgat gcaggcgaaa ggtataaagg ttttgccaca attcctgccc
      901 aaagctcatc tggaagagat cgctctgttc cagtactggc gaatcgacag ggtcatatcc
      961 tgcctgtgcc aggaacgtgc tcaggcgatc tattagccgg tgttgttgct ggtacgcacc
     1021 tggtaaaaga tcgtgcatac cacgcaagcg ctcaatacgc ttcttcatgg tcctctacta
     1081 atgctgttaa tggcttattc ggtaaccagc tttcgtccga cggccgaaga aactgcttgc
     1141 acctgcggca tcaagctctt gaactgatcg agcgtcagcg attgcgcccc atctttcaac
     1201 gcctcgtcgg ggttggggtg tacctcgacc agcagaccat cagcgcctgc agcgaccgag
     1261 gccagcgaca tggcgcttac caggtcgcgg tgcccggtcc cctggctggg gtcggcgatg
     1321 atcggcaggt gcgagtgtcg tttgatcacc ggaatagcgc tgatatccat cgtgttgcgc
     1381 gtcgtcttct caaaggtacg tataccgcgt tcgcacaaga tcaccctgga attcccctgc
     1441 gagagcacgt attctgctgc cagcagccac tcttcgatag tagccgacat cccgcgcttg
     1501 agcaacacag gtttctcaac acgccccacc tcgtcgagca gcatatagtt ttgcatgttg
     1561 cgcgtgccga tttgcaggat atcggcgtac tcgcagacca tttccacgtc ggtgggggtc
     1621 atgacctcgg taatgatcgc caggccaaac tcaccgcgcg ccttcgcgag cagcttgagc
     1681 ccttcttcgc ccatgccacg gaagccatag ggagaagtgg atggtttgaa ggctccgccg
     1741 cgcaggatca ctgccccctc ttttctgacc gcctcagctg tagtcat
//
LOCUS       DASZAK010001018         5365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_345382, whole genome shotgun sequence.
ACCESSION   DASZAK010001018 DASZAK010000000
VERSION     DASZAK010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5365
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345382"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VGT44_17940"
     CDS             complement(<1..596)
                     /locus_tag="VGT44_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HEV2582745.1"
                     /translation="MDIRVANAPCSWGALEFEGVESEIAYGQMLDELSDTGYVGTELG
                     DWGYMPTEPAILKEELQKRKLAMVGAFVPVALKNPEAHAQGEAHAVSVARLLAGVMDS
                     EGTNPPPLIVLADDNGTDPLRTEHAGRVTPEMGLNMAEWETYTRGAERIARVVRDATG
                     LRTAFHHHCAGYIETPEEIARFLDMTDSHLIGLVFDTG"
     gene            complement(615..2093)
                     /locus_tag="VGT44_17945"
     CDS             complement(615..2093)
                     /locus_tag="VGT44_17945"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEV2582746.1"
                     /translation="MIQDGQLLNYVGGKWKRSLAGEYLDVRNPATAETMVRVPLSSPD
                     EVDEAARLAQAAFADWRRTPPGDRIQYLFKLKKLLDDHFDEIARLTVDECGKTMAESQ
                     GELRRGIENVEVASGIPSLMMGYNVEDIARGIDEHMFRQPVGVVAAITPFNFPGMIPL
                     WFLPYAIACGNCFILKPSEKVPMTMSRVFDLIDELGLPPGVVSLVNGGKGVVDALLDH
                     PLVRAISFVGSSPVAKYVYSRAAANGKRAQCQGGAKNPVVVLPDADMEMATRIIADSS
                     FGCAGQRCLATSVTITVGEAGPVFREQISDVASGRKVGYGLEPEVEMGPVITVESKQR
                     IESLIGKGVNEGAKVLVDGRNTSIPGYERGFFVRPTVLDNVDARSELAGIEIFGPVLS
                     LMHAQTIDEAIELVNASPYGNMACLFTNSGAAARKFRYEARAGNIGINIGVAAPMAFF
                     PFSGWKNSFFGDMHAQGRDAIEFYTEKKVVVERWPREWSRKF"
     gene            complement(2123..3034)
                     /locus_tag="VGT44_17950"
     CDS             complement(2123..3034)
                     /locus_tag="VGT44_17950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2582747.1"
                     /translation="MNTQKAPLIDVQHVSKIFGSVIALKDISMAVRAGEVMCLLGDNG
                     AGKSTLIKILSGVHQPSEGRYLFEGKEMHFSSPRDALNMGIATVFQDLAMIPLMGISR
                     NFFLGAEPTKGWGPFRRFDVKHADQVTRDELSKMGIKVREMTQPVGTLSGGERQSVAI
                     ARAVYFGAKVLILDEPTAALGVKEAGVVLRYIAQARSRGLGVIFITHNPHHAYPIGDY
                     FTILNRGSSYGTFAKSEISREELTAMMAGGEALEELSHELAEFARTDEKAAASLAEAA
                     REFEIAAGDLHVEQLPTKFVDAEHQYE"
     gene            complement(3031..4071)
                     /locus_tag="VGT44_17955"
     CDS             complement(3031..4071)
                     /locus_tag="VGT44_17955"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2582748.1"
                     /translation="MSVIASERTARANPLRRLLVRPETGAVAGSIAVWIFFAIIGLAQ
                     NNGFVSSRGASSYLSVSAELAILAVPVALLMIGGEFDLSVGSTVGATGMIIAILSTQY
                     GWNIWAAIVAALIFAVLVGMLNGYLVMRTRLPSFIVTLGTAYILFGVTSGVTSALTGL
                     TVVSGVDQTPGYGAAHAIFATALGGGQQFPIEIVWALALVALATWLLLGTSFGNWIFG
                     VGGDLQAARNTGVPVTRVKILLFIGTALGACLLAIIQVVTFTTADVLRGQNQEFVAIV
                     AAVVGGVLLTGGYGSAIGAMFGALAFGIVEIGIVFSGVNAEWYKAVIGLILLLAVLLN
                     SYVRKLAMEARR"
     gene            complement(4149..5159)
                     /locus_tag="VGT44_17960"
     CDS             complement(4149..5159)
                     /locus_tag="VGT44_17960"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2582749.1"
                     /translation="MNRLFKKLIILVVMLPLVLVACGGSSTTNNQSSTSGGNSSRANI
                     KFYVITHGQATDPFWSVVKKGVDQAAHDMGVQAIYEGPASGTFDVVAMAHLIDTAVAA
                     HPDGIVVSIPDANALGPSIKAAVAAGIPVISINSGSDVAKSLGVLVHVGQTEEQAGIG
                     GGLKMGAAGVKNALCINQEVGNAALALRCKGFQEGLTQSDGGKMKVVGVTLSNPTQTQ
                     QTIEAALQHDPTIDGILTLGPTGATPAIAALKDLNKLGQIKLATFDLSSDVLNAIKAG
                     QMLFAIDQQQYEQGYLPIVLLTLYKTNLNTIANDILQTGPGFVTKDNVSQVIQLTAQG
                     TR"
BASE COUNT         1162 a         1653 c         1406 g         1144 t
ORIGIN      
        1 tggcccgtat cgaacaccag cccgatcagg tgggagtcgg tcatgtccag gaaacgagcg
       61 atctcttccg gtgtctcaat gtacccggca caatggtgat gaaaagctgt gcgcagacca
      121 gtcgcatcgc gcaccacccg cgcgatgcgc tcagcaccgc gcgtatatgt ttcccactcc
      181 gccatgttca ggcccatctc gggcgtaaca cgtccagcat gctccgtgcg cagagggtcg
      241 gtaccattat catcggcaag cacaatcagc ggcggtggat tcgtgccctc gctgtccatc
      301 acaccagcta gcaatcgagc aacactcaca gcatgtgctt ccccctgagc atgtgcttcg
      361 gggtttttca gcgcgaccgg cacgaatgcg cctaccatcg ccaatttgcg cttttgtagt
      421 tcctctttga gaatagccgg ctcagtgggc atatagcccc aatcgccaag ctcggtgcct
      481 acgtatcctg tgtcgctcaa ctcgtcgagc atctgcccgt aagcgatctc actctctact
      541 ccttcgaatt cgagcgctcc ccacgaacag ggcgcgttgg caacacgaat atccatcttt
      601 tttgatcctc tcatctaaaa cttgcgcgac cactcgcggg gccagcgctc gaccacaacc
      661 ttcttttctg tgtagaactc gatggcatcg cgtccctggg catgcatatc gccgaagaaa
      721 ctgttcttcc agccagagaa agggaagaaa gccatcggag cagcaacgcc aatgttgatg
      781 ccgatgttgc ctgcccgtgc ctcgtagcgg aatttgcgtg ccgcggcacc gctgttcgtg
      841 aacaggcacg ccatattacc atagggggag gcgttcacca gctcaattgc ttcatcaatt
      901 gtctgggcgt gcatcaggct cagaaccggc ccgaagatct caatcccggc aagctcacta
      961 cgcgcgtcga cgttgtccag gactgtcggg cgcacgaaaa agccccgctc gtagccaggg
     1021 atcgatgtat tgcgcccatc gacgaggact ttggcacctt cgttcacgcc cttgccgatc
     1081 agcgattcga tgcgttgctt gctctcgacc gtgatgaccg gccccatctc gacctctggc
     1141 tcgagtccgt agcctacctt gcgccctgac gccacgtcag agatctgctc cctgaagacc
     1201 gggccggcct caccgaccgt aatcgtcacc gacgtcgcca ggcagcgctg tcccgcgcag
     1261 ccaaaggacg aatcggcgat gatgcgggtg gccatctcca tatcggcgtc gggcaagaca
     1321 acaacgggat tcttagcccc tccctggcat tgggcgcgct ttccattggc cgccgcccgg
     1381 ctgtacacgt actttgccac aggtgaggag ccgacgaagg agattgctcg tacaagcggg
     1441 tggtcgagca aggcatcgac gacccctttg ccaccattga ccaggctaac tacacctggc
     1501 ggcaggccca actcgtcgat caggtcaaag acccggctca tcgtcatcgg cactttctcc
     1561 gatggcttga ggatgaagca attgccacag gcgatagcat agggcaggaa ccagagcggg
     1621 atcatgccag gaaagttgaa cggtgtaatc gctgcaacaa caccgaccgg ctggcgaaac
     1681 atgtgttcat caatgccgcg cgcgatatct tcaacattgt agcccatcat cagtgatgga
     1741 atgccactcg caacctcaac attctcgata ccccgtctca gctcgccttg cgactctgcc
     1801 atcgtcttgc cgcactcatc gactgtcaga cgagcgatct cgtcgaaatg atcatccaac
     1861 aacttcttca gcttgaagag gtattggatg cgatcgcccg gaggcgttcg ccgccagtct
     1921 gcgaaggcgg cctgggctaa ccgagccgct tcgtccacct cgtcagggga agagagtggc
     1981 acgcgcacca tcgtctcggc ggtcgcgggg ttacgcacgt cgagatattc gccggcgagc
     2041 gagcgcttcc atttacctcc tacatagtta agtaattgtc catcctgtat catgggttcc
     2101 cttctccatc tagcggctga aactactcgt attgatgttc cgcgtccaca aatttcgtcg
     2161 gaagctgctc aacgtgcaga tctcccgctg ctatctcaaa ttcacgagcg gcctcggcca
     2221 ggcttgcggc agctttctca tccgtgcgcg cgaactcggc cagttcgtgg ctcaactcct
     2281 ccagggcttc gcctccagcc atcatcgctg taagttcctc gcgactgatt tcggatttag
     2341 cgaacgtgcc atagctgctc ccgcgattga ggatcgtgaa gtagtctcca atcggatacg
     2401 cgtgatgggg gttgtgtgtg ataaagatga ctcccagtcc tcgcgagcga gcctgggcga
     2461 tgtagcgcag caccacacct gcctccttca cacccagagc cgccgttggt tcgtccagaa
     2521 tcaacacctt ggcaccgaag tacacagcac gcgcaatggc aacagactgg cgctcaccac
     2581 ctgagagcgt acccaccggt tgtgtcatct cgcgcacctt gatacccatc ttcgacagct
     2641 catcgcgcgt cacctggtcg gcgtgcttca cgtcgaaacg acgaaacggt ccccagccct
     2701 ttgttggctc cgcaccaagg aagaagttgc gcgaaatgcc catcaaagga atcatcgcca
     2761 ggtcctggaa gacggtggct atacccatat tcaacgcgtc gcgcggcgag gagaaatgca
     2821 tttcctttcc ctcgaacagg tagcgaccct cgcttggctg gtgaacgccg gagaggattt
     2881 tgataagcgt tgacttgcct gccccgttgt caccaagcaa gcacatcacc tcgcctgctc
     2941 gtactgccat ggaaatgtcc ttcagcgcaa ttacgctgcc gaagattttt gagacgtgct
     3001 gcacatcaat taaaggagct ttttgtgtgt tcatcgtctt gcctccattg ccagtttgcg
     3061 tacgtagctg ttaagcagca cagctagcaa cagtattaac ccaatgacag ccttgtacca
     3121 ctcagcattg acgcccgaga agacgatgcc tatttctacg atgccaaagg ccagcgcccc
     3181 gaacatggca ccgatagctg aaccgtagcc acccgtaagc aatacccctc cgacgaccgc
     3241 cgcgacgatc gcaacgaact cctggttctg cccacgcagg acatccgcgg tggtaaaggt
     3301 cacaacctgg ataatcgcaa gcaggcaggc accaagagcc gtgcctatga acagcagaat
     3361 cttcacccgg gtgaccggta caccggtgtt gcgtgccgcc tggaggtccc cacccacgcc
     3421 aaatatccag ttcccgaaag aggtgcccag gagcagccag gttgccagcg ctaccagcgc
     3481 cagcgcccag actatttcga tgggaaattg ctggccgccg cccagcgcgg tggcgaagat
     3541 cgcgtgagca gcgccatacc ctggtgtctg gtcgaccccg ctcaccacgg ttaagccggt
     3601 cagcgcggag gtgacacctg aggtcacgcc gaacaggatg tacgcggtgc ccagggtgac
     3661 gataaaagac ggcaggcgag tgcgcataac caggtagccg ttcagcatgc ctaccagcac
     3721 cgcaaagatc agggccgcga caatcgccgc ccagatgttc caaccgtact gagtgctcaa
     3781 gatggcaatg atcataccag tagcgccaac cgtcgaacca accgacaggt caaattcccc
     3841 gccaatcatg agcaatgcaa cgggcactgc caggatggcc agctcagccg aaacgctcag
     3901 gtagcttgat gcgcctcttg aactaacaaa accattgttc tgtgccagtc ctatgatggc
     3961 gaagaagatc cacaccgcaa ttgaaccggc aaccgctcct gtttccgggc ggaccagcag
     4021 ccggcggagc ggattggcgc gcgccgtacg ctcactcgca ataacgctca tagttacctc
     4081 cttttctgta aatggtgttg gagtcaatgc tgagccagtg gccttcttga ctccaacacg
     4141 gaaacttgct agcgcgtccc ttgagccgtc aactggatta cctggcttac gttatccttt
     4201 gtgacaaagc cggggccggt ctgaaggata tcattcgcga tagtgttgag attcgtcttg
     4261 tacaaggtca acaagacaat aggtaagtat ccctgctcat actgctgctg atcaatagca
     4321 aacagcatct gaccagcctt gatcgcgtta agcacatccg acgacaggtc gaatgttgcc
     4381 agcttgatct gtcccagttt gttcaggtcc ttgagagcag cgatggccgg ggtggcgccc
     4441 gtggggccaa gcgtcaggat gccatcaatc gtgggatcgt gctggagcgc agcctcgatg
     4501 gtctgctggg tctgcgtcgg gttgctcagg gtaacgccga ccactttcat tttgccgcca
     4561 tctgactgcg tcaaaccttc ctggaagcct ttacagcgca gtgcaagagc ggcattaccg
     4621 acctcctggt tgatacagag ggcgtttttg acaccagctg cgcccatttt caggccaccg
     4681 ccaatgccag cctgctcctc ggtctgcccg acatgcacca acactcccag gctcttggcg
     4741 acatcagagc cagagttgat cgagatcacc gggataccgg ctgccacagc cgctttaatc
     4801 gatgggccca gggcgttggc atctgggatc gagacaacta tcccatccgg gtgtgctgcc
     4861 accgcggtat caatgagatg ggccatcgct accacatcga aggtacctga ggctggccct
     4921 tcatagatcg cctggacacc catatcatgg gccgcctggt caaccccttt cttgaccaca
     4981 gaccagaatg ggtccgtagc ctggccgtgt gtgatcacgt agaacttgat gtttgcgcgt
     5041 gacgaattgc cgccactggt tgacgactgg ttgtttgtcg tactacttcc gccacaggcg
     5101 accaggacga gaggaagcat tactaccaaa atgatgagtt ttttgaagag tcggttcacc
     5161 ttgtcctcct taattacggt tgagacctac tatgcttaca atgaatttcc gttgcgaacg
     5221 gctccgaaac gattgacctc gtgcagatac gagccgttca acttccatgg caactttttt
     5281 atcctctgtt cgttccctcc tttcccgaaa tacgtgggtt ccattttcaa tctatcgtcc
     5341 agcaccggca ggtacagcat gccac
//
LOCUS       DASZAK010001019         3894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_487831, whole genome shotgun sequence.
ACCESSION   DASZAK010001019 DASZAK010000000
VERSION     DASZAK010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487831"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VGT44_17965"
     CDS             complement(<1..812)
                     /locus_tag="VGT44_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEV2582750.1"
                     /translation="MATRTIAEGITLERRKRFSESYIGEWIMTTDHKKLGIMYIITAF
                     FFFLVGGTEALLIRTQLAVPNGKVLTPEIYNQVFTMHGTTMIFLFVMPMLTGLGNYIV
                     PLMIGARDMAFPRINAFGYWVVLFGGLFLNISFLFGQAPNAGWFNYAPLNELTTGCGS
                     TAYVCTPGMNTDFWALGILMLGISSIGGSLNFVVTILKLRAPGMTINRMPLFTWMTMI
                     TAFLLLFALPSVTAASIMLLLDRHLGTHFFQAGVGGDPLLWQHLFWSFGHPEV"
     gene            complement(850..1956)
                     /gene="coxB"
                     /locus_tag="VGT44_17970"
     CDS             complement(850..1956)
                     /gene="coxB"
                     /locus_tag="VGT44_17970"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEV2582751.1"
                     /translation="MPKTRKVKRWGIISTAILLSSVLLAACGGTPPSILDTHGPVAAK
                     EAGLFWFILAVATFVFVVVEGVLIVSIVRFRERPNAPEPKQLHGNNTIEIIWTVAPSL
                     FLFAVLIGTIYTMFNLTNISGQGRTEEITVVGHQWWWEFDYKIEGIVTADELVVPQGT
                     LIHTVLESNNVIHSFWVPQITGKTDVVPGHMNDKLFKADDTGTFRGLCTEYCGLEHAH
                     MAFDLVVMTPANYQTWVSGQQLKAQIPASGAAQAGYKLFTGSGGCVGCHGIVGVNLNS
                     FSDPVGNSLVGPNLTHFGSRDLIAGGVLSWSASSCVVTTDAQGNPAIANPSACGLYQW
                     LQDPQAVKPGNDMNIRSLSPTEIAQLIAFLESLQ"
     gene            2195..2602
                     /locus_tag="VGT44_17975"
     CDS             2195..2602
                     /locus_tag="VGT44_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582752.1"
                     /translation="MSQSALDLDETKPSAQILDSIKSFDDTAKQLVTMEGILVGLYFN
                     AFAFAKVAVGPGWQLIVYLLPVVLLVASLLSALRVFFPAGYLELAQEEAHAQRILYSR
                     VAQHKITWFRYASIFAVLSIIGVFFALMTYLLR"
     gene            2618..>3894
                     /locus_tag="VGT44_17980"
     CDS             2618..>3894
                     /locus_tag="VGT44_17980"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEV2582753.1"
                     /translation="MGLIHGGWLVAKTLKREGVEVVFTLSGGHIAGIYDGCVREGIRV
                     VDTRHEQAAVHAAEGWAKSTRKPGVALLTAGPGVTDGVTGVANAYLAGSPVLVIGGAA
                     PLGLWDRGALQEMNQIDLLRPITKWTRTVHETARLAEYTAAAFRQMLSGKPGPVFLEM
                     PMDVLNNFADTDTLFDPGDPAHYRYTGRTAPDPDIVARAAALLEKAQKPVIMAGTAVW
                     WCDAAEPLRLLAERLNAPVYLNGAGRGCLPPTHPLFFSASRRKALEGADTILAIGTRM
                     DFRLNHGQPPLIPAQANLIWFDLAGEDVGVNRGAAAALVGDVGLAMRQLTGAVKQAPA
                     GEWLAFIRGEEHKAQERDEALMRSDAEPIHPMRLCGEIRNFIDEHTTVVGDGGDIVSY
                     GGRVINVFKPGYWLDPGPMGCLGTGTGFAMAAQL"
BASE COUNT          831 a         1194 c         1100 g          769 t
ORIGIN      
        1 acctctggat gtccaaacga ccagaacagg tgctgccaca acagtgggtc gccccccaca
       61 cctgcctgga agaaatgcgt tccgagatgg cggtcgagca gcagcatgat gctcgccgcc
      121 gtcacgctgg gcagggcgaa gagcagcagg aacgccgtaa tcatcgtcat ccaggtgaaa
      181 agcggcatac ggttgatcgt catgccgggc gcgcgcagct tgaggatcgt cacgacaaag
      241 ttgagcgaac cgccgatgga cgagatacca agcatcagaa tgcccaatgc ccagaagtcc
      301 gtgttcatgc ccggtgtgca aacgtaagcc gtcgagccac agccggtggt cagttcgttg
      361 agcggcgcgt agttgaacca gcccgcgttt ggagcttgcc caaacaggaa gctgatattc
      421 aagaaaaggc cgccaaacag cacgacccag tagccgaacg cattgatgcg cgggaaagcc
      481 atatcgcgcg caccaatcat gagcggcaca atatagttac ctaagcccgt cagcatcggc
      541 ataacaaaca aaaagatcat ggtggtgcca tgcatggtaa agacctggtt ataaatctcc
      601 ggggtgagga cttttccatt tggcactgcc agctgggtgc gaatcaacaa cgcctcagtt
      661 cctccaacca ggaagaaaaa gaaggcggtg atgatataca tgatgccgag cttcttatga
      721 tcggtcgtca tgatccattc gcctatatag gattcgctaa agcgcttgcg gcgttctagc
      781 gttatgcctt cggcgatagt acgggttgcc ataggcagtt cctccatcta gtaataggag
      841 tgaagttcat cactggagac tttccaggaa cgcaatcaat tgcgcaatct cggtcggcga
      901 gagtgaccgg atgttcatat cattgccggg cttgacggcc tgtgggtctt gcagccactg
      961 atagaggcca catgccgacg ggtttgcgat ggcaggattg ccctgcgcgt ccgtcgtcac
     1021 cacgcacgag ctcgcgctcc acgacagcac cccaccggcg atgaggtcgc gactgccgaa
     1081 gtgcgtcagg tttggcccaa ccagcgaatt cccaaccggg tcgctgaagc tattcaggtt
     1141 gacgccgacg atgccgtgac agcccacgca gccgccagag ccggtgaaaa gcttgtagcc
     1201 tgcctgcgcc gccccgctcg ccggaatttg cgccttcagc tgctgtcctg atacccaggt
     1261 ctggtagtta gccggagtca tcaccaccag atcaaaggcc atatgcgcat gttccaggcc
     1321 gcagtactcc gtgcaaagac cgcgaaaggt gccagtgtcg tctgctttga acagtttatc
     1381 gttcatatgg ccgggaacca cgtcggtctt gcccgtaatc tgaggaaccc agaagctgtg
     1441 gatcacgttg ttcgactcca gcacagtgtg gatcagcgta ccctgcggca ccaccagttc
     1501 atcagccgta acgatgccct cgatcttata atcgaattcc caccaccact ggtggccgac
     1561 taccgtgatc tcctcggtgc gtccctgccc tgagatattg gtcaggttga acatcgtata
     1621 gatcgtgcca atcagcaccg cgaacaggaa cagagagggc gcgacggtcc agatgatttc
     1681 aatagtgttg ttgccatgca actgcttcgg ctcaggcgca tttggccgct cgcggaagcg
     1741 cacgatggac acgatcagga cgccctccac caccacgaaa acaaaggtgg cgacagccag
     1801 gataaaccag aagagtcccg cttctttcgc ggcgaccggg ccgtgcgtat ccagaatgga
     1861 cgggggagta ccaccacagg cggcaagtag aaccgaggat aaaagtatcg cggtggagat
     1921 gatcccccaa cgcttcacct tgcgtgtttt tggcatcctc acttctcact ttcctagaaa
     1981 atatagtttt gacgatggcg agcattgcta gacacactca ttccatagta cctgtaagtg
     2041 tagacatcct gcctgaaaga gaacttacgt tttttcgcca tcaccttaca gcagcctgaa
     2101 tagctgcccc catcccctac ggacaaatat cctctaaata gtgtatcata acctgaaagg
     2161 acgcaaatac ggtatgtcga aaggagcaac agccatgtct cagtcagcgc tagacctgga
     2221 tgaaacgaag ccgtcagcac agattctcga ctccatcaaa tccttcgatg atacggccaa
     2281 acagctcgtc acaatggagg gcattctggt cggactctat ttcaacgcct ttgcttttgc
     2341 caaagttgcc gtcggccccg gctggcagct catcgtctac ctcctgccag ttgttctgct
     2401 cgtcgccagt ctgctctccg cgctgcgcgt cttctttccc gccgggtatc ttgagcttgc
     2461 tcaggaggag gctcatgctc agcgtattct ctacagccgc gtcgctcaac acaaaattac
     2521 ctggtttcgc tacgcctcga tctttgctgt gttgagcatc atcggtgtgt tcttcgcctt
     2581 gatgacgtat cttttgaggt aataaaggag tttccacatg ggactgatac atggcggctg
     2641 gctggtcgcc aaaaccctta aacgcgaggg cgtcgaggtg gttttcaccc tgagcggcgg
     2701 ccatattgcc gggatttacg acggctgtgt gcgcgaaggc atccgcgtcg tcgatacgcg
     2761 ccacgagcag gcggccgtcc atgccgccga gggctgggcc aaaagcacgc gcaagcccgg
     2821 cgtagccctc ctgaccgccg ggcctggtgt gacggacggc gtaacgggcg tcgccaacgc
     2881 ctacctcgcg ggcagtcccg tgctggtcat tggcggcgcg gcgccactcg gcctatggga
     2941 tcgcggcgcg ttgcaggaaa tgaaccagat cgacctgcta cgacccatca ccaagtggac
     3001 acgcaccgtg catgagaccg cgcgcctggc cgaatacacc gccgccgctt ttcgccagat
     3061 gctcagcggc aagcccggcc cggtcttcct cgagatgccg atggacgtgc tgaacaactt
     3121 cgccgatacc gacacgctct tcgaccccgg cgatccagcg cactaccgct atactggccg
     3181 caccgcgccc gatcccgaca tagttgcgcg agccgccgcc ctgctggaaa aagcgcagaa
     3241 gcccgtcatc atggcaggca ccgccgtgtg gtggtgcgac gccgctgaac ccctgcggct
     3301 gctggcggaa cgcctcaacg cgcccgtcta cctcaacggc gctggtcgcg gctgcctgcc
     3361 gcccacgcat cccctcttct tcagcgcgtc gcgccgcaaa gccctggagg gtgccgatac
     3421 catcctggcc atcggtacgc gcatggactt ccgcctcaat cacggtcagc cgccgctcat
     3481 cccggcgcag gctaacctga tctggttcga tctcgcgggc gaggatgtgg gcgttaatcg
     3541 cggcgctgcc gctgccctgg tcggcgacgt gggcctcgcc atgcgccagt tgaccggggc
     3601 cgtcaaacaa gcgccggcag gtgagtggct cgctttcatt cgcggcgagg agcacaaggc
     3661 gcaagaacgc gacgaggcgc tgatgcgttc cgatgccgag ccgatccatc ccatgcggct
     3721 gtgcggcgag attcgcaact tcatcgatga gcacacaacc gtcgtgggcg acggcggcga
     3781 tatcgtcagc tacggcggca gggtcatcaa cgtcttcaag cctggctact ggctcgatcc
     3841 cggcccgatg ggctgcctgg gaaccggcac cggcttcgcc atggccgcac agct
//
LOCUS       DASZAK010001020         1610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_98696, whole genome shotgun sequence.
ACCESSION   DASZAK010001020 DASZAK010000000
VERSION     DASZAK010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98696"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /gene="hrcA"
                     /locus_tag="VGT44_17985"
     CDS             complement(<1..871)
                     /gene="hrcA"
                     /locus_tag="VGT44_17985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV2582754.1"
                     /translation="MTQREQEILHSIVECYIHTGEPVASRTIARQRRDGLSAATVRNV
                     MADLCDEGYLSQPHTSAGRIPTEKAFRSYVQSLARGRLMMTELQRVRGELSQMDTMYA
                     RVECSSRLLTEMTRSIGITAAIPASSQTLDQIELVSLADQRVLMIVVTRDRMVRNRVV
                     TLDEFVSQDELASIRNYINRNFSGWTLSEIRTDLERRLERQNAAYDALLKRVTLLYSK
                     GLLDIELTPEIHMEGASNLVGLDLHLTREKLRDLFHTLEEKKRMLQLLDRFLEQPGGE
                     LAVQVGLADAHPSM"
BASE COUNT          354 a          467 c          473 g          316 t
ORIGIN      
        1 gcatgctggg atgcgcgtcc gccaatccca cctggaccgc cagttctccg cccggttgtt
       61 cgagaaaacg atccagcagc tgcaacatcc gctttttctc ttccagcgtg tggaacaggt
      121 cgcgcagctt ctcacgcgtc aaatgcaaat ccaagccgac cagattactg gcgccctcca
      181 tgtgaatctc cggagtcagc tcgatatcca gcaggccttt ggagtaaagc aaggtgacgc
      241 gtttcagcag cgcgtcataa gcggcgtttt ggcgctccag gcggcgctcc aggtcggtgc
      301 ggatctcgga gagcgtccag ccgctgaaat tgcggttgat gtagttgcga atggaagcca
      361 gctcgtcttg cgaaacaaat tcatccaggg ttacgacgcg gttgcgcacc atgcggtcgc
      421 gtgtcaccac gatcatcagt acgcgctgat cggccaggct gaccagttcg atctgatcca
      481 gagtctggct ggaggccgga atagcggccg tgatgccgat gctgcgcgtc atttccgtca
      541 gcaggcgcga ggagcattcg acgcgcgcat acatggtgtc catctggctc aactcgccgc
      601 gcacgcgctg caactccgtc atcatcaggc ggccgcgcgc gagcgactgc acgtaactgc
      661 ggaatgcttt ttcggtgggg atgcgcccgg cggaagtgtg cggctgcgaa agatagccct
      721 catcgcacag gtcggccatg acgttgcgca ccgtggccgc gctgagtcca tcccggcgct
      781 ggcgcgcgat tgtacgcgag gccacaggtt ccccggtgtg gatgtaacat tccacgatgg
      841 aatgcagaat ttcctgttcg cgctgtgtca tgggtgaacc tgccggtccg ccggcttggg
      901 agagcaccct cctacttatt gattgtaaag ggtttcgttt tggattttca agctccacgg
      961 cgaaaaggcc atggagggaa tttcgatgac ggagaactgg ggctaagcgg cacgaaccgg
     1021 ccgaccagta ctggaaagtt tgcataggcg atgccattgg ccaaggcgaa acaccccgat
     1081 tcgagcatgt attcgctcat tgttctgggt cccaacccaa tgttccacaa cagcaatgat
     1141 cccggcgaca ccgcgagaac aggaagcaac caccaaccgc tcagtttgaa gactccccaa
     1201 aagatttggc aaactattgt gacgccagcg ccaagcgaga atcgcttcag ggactgccgt
     1261 gcaccttggt aacggtaagg agctaacacg gcgacgcaca tgaatgcgat gaagtagctc
     1321 agctggaccc acgcaggaaa agggcgaaga atcaggaagg gaagaaagaa agccgcgccg
     1381 aaacataaca gcgttaccag aggaagcagc agccagacgc tacgtccgta gtttcgggac
     1441 tcggacatct ggaatctggc ctcccagtcc ttctccgtat tgatcagagt aacgaaacac
     1501 gatcccgatc gggcgaggag ttatcttcgc tttgctgacc ggaacgcctg cattcgctcg
     1561 cgcggctcgt cgctcttcca cgcctcgacg aaggactcga ttccggcgcg
//
LOCUS       DASZAK010001021         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_666575, whole genome shotgun sequence.
ACCESSION   DASZAK010001021 DASZAK010000000
VERSION     DASZAK010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666575"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="VGT44_17990"
     CDS             <1..873
                     /locus_tag="VGT44_17990"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2582755.1"
                     /translation="AQPVLPEKEASIITTLEFLEQNLRSQGFVPLQPGSLSALAQETT
                     QAPQSTSPLALSEASTPVDQLVDQTTPPQLAFNERPDVPIQQTSQVKQAQQFEPVPFE
                     PAIASAQPISPNSDIPPVEAAPDETPAAAEPPQPIPPMVEPMPVLSLDTLLDSELETT
                     MRRPAVRLQPMQAESSQADRPTVARNMDYSAKSADSKLTNKERLVRGYQFQLAGSYDD
                     AMQEYRTLIRTSPESLGEVISNVRALLKLAPKYTVGYRVLGDAYMRQGEYLQAMEAYN
                     KALTIAKKAKSQSR"
     gene            895..1242
                     /locus_tag="VGT44_17995"
     CDS             895..1242
                     /locus_tag="VGT44_17995"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEV2582756.1"
                     /translation="METVLQRLMDLDEITGTLVIGKDGLIVAGALPGDEEDMLSAMTA
                     AVFGSIASYTSQTNHGETRHVIIETERGSIQFAESHDLILVVTTQNASNAGRVKFEMQ
                     KACKHLAELVTAY"
     gene            complement(1316..1588)
                     /locus_tag="VGT44_18000"
     CDS             complement(1316..1588)
                     /locus_tag="VGT44_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582757.1"
                     /translation="MSDTIEINPSENSNWRKHKNACPFYRERWFPDNDTSAGEPMYQV
                     FCMQGTPPITWEEQDRCLHSKTCCWRLADGKKRAAKEPQASSASTH"
     gene            1688..>2421
                     /locus_tag="VGT44_18005"
     CDS             1688..>2421
                     /locus_tag="VGT44_18005"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEV2582758.1"
                     /translation="MIEPVQDKQLLDSETRAGLAPWTWGQTWLGIALTMLPWMALAIA
                     LSSSGNAAGGSKAPLTFQIDLVSALITLIFSALVEGAFLIAPFAIANRVYRAIKSHMQ
                     LVLASLGLRKFSIRQAALWIIGFMVLIFVVNILYSTIITTFHLNLQTNDQVILARSKQ
                     APITTYATLIVAVFVAPFCEEVFFRGFVFPGLRNKMPVALAIITSALLFAIAHVDPAS
                     FAVLLAIGLMLGVMRWRTNSIWPGILL"
BASE COUNT          541 a          747 c          607 g          526 t
ORIGIN      
        1 gcacaaccag tccttcctga aaaggaagca tccatcatca ctaccttgga gttcctggaa
       61 cagaatctgc gctcgcaagg ttttgtgcca ttgcagcctg gttcgttgtc agcccttgcg
      121 caggagacga cgcaagcgcc gcaatcaacg tcgccgctcg ctttgagcga agcaagtact
      181 ccagtcgacc agctggtcga tcagacaacg cccccgcagc ttgccttcaa cgagcgtcca
      241 gacgttccta tccagcaaac atcccaggtt aaacaggcac aacaattcga gcctgttcct
      301 tttgaaccgg ccatcgccag cgctcagcct atctcaccca atagcgacat accgcccgtc
      361 gaggctgccc ccgatgagac acctgctgcg gctgaaccac ctcagcccat tccgccaatg
      421 gtcgaaccaa tgccggtgtt gagcctggat acactcctcg atagcgagtt ggaaactact
      481 atgcgccgac cagccgttcg attgcagcca atgcaggccg agtcatcaca ggctgatcgg
      541 ccaacagtag ccaggaacat ggactacagt gcgaaaagcg ctgacagcaa gctcaccaat
      601 aaagagcggc tcgtgcgcgg ctaccagttt caacttgccg ggtcctacga tgacgccatg
      661 caagaatatc gcaccctgat tcgcacctcg cccgaatcgc tgggcgaggt catcagcaac
      721 gtgcgcgcct tgctcaagct cgcgccaaag tacacggttg gctatcgtgt gttgggtgat
      781 gcctacatgc gccagggcga atacctgcaa gcgatggagg catacaacaa ggcgctgacc
      841 attgcgaaga aggccaaaag ccagagccgt taattacagt gaaaggagca aaagatggaa
      901 accgttcttc agcggttgat ggaccttgat gagatcaccg gcacgcttgt gatcggtaaa
      961 gacggcctga tcgtcgccgg cgcgctgccc ggcgatgaag aggatatgtt gagtgccatg
     1021 accgcagccg tctttggctc tattgccagc tacacctcgc aaaccaatca cggcgaaacc
     1081 cgccacgtca tcattgagac cgaacgcggc tcaatacagt tcgctgagtc ccatgacctg
     1141 atcctggtcg tcaccaccca aaacgcgagc aatgccgggc gtgtgaaatt tgaaatgcag
     1201 aaagcctgca agcacctggc tgaactggtg acggcctact aaacacagtg gtcttaccgt
     1261 ttatcgtttt gaaacacccg tgtagaggca ccatgcactg ccttcgtatg ccgatttagt
     1321 gggtagaggc cgaggacgct tgcggctcct tcgccgctct ctttttgccg tccgccagtc
     1381 gccagcagca ggttttgcta tgtaaacagc gatcctgctc ctcccaggtt attggcggag
     1441 tgccttgcat acaaaacacc tggtacatcg gctcccccgc cgacgtgtca ttatcaggaa
     1501 accagcgttc acgatagaat gggcaggcat tcttgtgctt tcgccagttg gaattttcag
     1561 atggattaat ttcgatcgta tcggacaagt agaaatcctt ccctggaaga tgagctagtt
     1621 gtcattggac tttgaagtag tataccatat aacataatgc ctgactagaa aatttcggag
     1681 caatacattg atcgaaccag tacaggataa acagctactc gattccgaga cgcgcgccgg
     1741 gctggctccc tggacctggg ggcaaacctg gctcggcatc gccctcacta tgctgccgtg
     1801 gatggccctc gccatcgcac tgagcagttc gggcaacgcg gctggtggta gtaaggcacc
     1861 gttaaccttc cagatcgacc tggtcagcgc cctcatcacg ctgatcttct ccgccctggt
     1921 cgaaggcgca tttctgatcg ccccatttgc catcgccaat cgcgtttatc gcgccatcaa
     1981 gtcacacatg cagctggtgc tggcctcgct gggcctccgc aagttctcga tccgccaggc
     2041 cgcgctctgg attattggct tcatggtcct tattttcgtc gtcaacatcc tctactctac
     2101 aatcatcacc acctttcatc tcaacctgca aaccaatgac caggtgatcc ttgcgcgcag
     2161 caaacaggcg cctatcacca cctatgcgac cctcatcgtg gccgttttcg tcgcgccctt
     2221 ctgcgaagag gtctttttcc ggggatttgt cttccccggc ctacgcaaca aaatgcccgt
     2281 cgccctggct atcatcacca gcgcgctgct cttcgccatc gcgcacgtcg accccgcctc
     2341 gttcgccgtg ctgctagcta ttggcctgat gctcggcgtc atgcgctggc gcaccaactc
     2401 gatctggcca ggcatactac t
//
LOCUS       DASZAK010001022         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_382638, whole genome shotgun sequence.
ACCESSION   DASZAK010001022 DASZAK010000000
VERSION     DASZAK010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382638"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VGT44_18010"
     CDS             <1..365
                     /locus_tag="VGT44_18010"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2582759.1"
                     /translation="FMRDNTMDKFPEAERQRMQRVHQYGDEQIQALFRHGQSFKDTYD
                     DMNFTPPYLSTIAARTLIAYGDRDPLLPVHIALEQYAAIPHSYLWVVPNGGHVPVFSG
                     ISFMEAVLPFLRGDWEQE"
     gene            373..1464
                     /locus_tag="VGT44_18015"
     CDS             373..1464
                     /locus_tag="VGT44_18015"
                     /inference="COORDINATES: protein motif:HMM:NF017832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HEV2582760.1"
                     /translation="MLVAYGPGDQPVVADETPLEQLRQLSQERLLICPNCRGIVHVRG
                     GPEKRIQLHFAHQRGECAWSTEAESVRHMRGKIVLATWLRAQFPQASVTLEERLPGSN
                     RIADIFMQHPDGHRQALEFQCAPLDLDEWQRRHQAYRAAGICDTWIIGENRREKQEAF
                     LEAILGTAREILFLDPLADEPRTWLRWPATRAMIQAWQREATLRPSFEGWVGRLGSGV
                     TLVGALMTVQIDRQGLLLHPRRTALLARLSLLERMTGEPAIDEAALVEYLEPAIGAQA
                     IQRVILPLLRAYTRDPDLLRRYNYGRGQHGQPPGEADLLRVQRARAWLEALAQQDYDA
                     ERLRALARDIPFVGPYAALAGYLEMLVEL"
     gene            complement(1461..2816)
                     /locus_tag="VGT44_18020"
     CDS             complement(1461..2816)
                     /locus_tag="VGT44_18020"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEV2582761.1"
                     /translation="MNTQDVVRQAREANLRLVRFLYCDNGGIIRGKTTHGSKLDSRMK
                     EGIGQTLAMQAFTGVETLASVEGMGPVGEFRLIPDPSTFTILPYVPSSGSMMCDMILT
                     SGQPWDACPRTFLKRMGARLAEQGMRAEAAIEHEFYLAREENGKYVPADRSLCYSSIG
                     LDDQAEVIDSILEALESQGVSIELFHTELGPGQQELSIRHADALRAADNACLVRETVR
                     GVARNFELLASFAPKPFLDQAGSGAHIHLSLWDNSGPNGRNLFYDANQRGGLSETGKY
                     FTGGVLRHIRGLLALTCGSPNSYRRLLPHFWSSAYAVYGFDNREGAIRVPSVFWGREA
                     TSINLELKPADHSGNPYLAIGALIAAGLDGIANKIDPGDPQQIDPGNYSDAEREQRGI
                     QRLPTTLDEAVAELERDEVLTGALGPLLATSYIAVKRSESAFFKEQSPEEETKQHFYK
                     Y"
     gene            3067..3621
                     /locus_tag="VGT44_18025"
     CDS             3067..3621
                     /locus_tag="VGT44_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2582762.1"
                     /translation="MDEPPMETTWRTALWRQFGAAIDMLENALLACPASLWNERLWSD
                     QEQPDYPTFWYLTYHTLFWLDLYLTGKTEGFAPPAPFRNPFDPKSAFPTQPYTRDELH
                     TYLKHLRQKCQSTITELSDEQGHQPFHFPWDIEHPISYGELQLYTMRHVQEHAAQLSL
                     FLGQHGIPDDALDWVGRAKGDPDS"
     gene            3768..>4255
                     /locus_tag="VGT44_18030"
     CDS             3768..>4255
                     /locus_tag="VGT44_18030"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV2582763.1"
                     /translation="MHKNEQEVMMLTINTETTRRFILGKQGLWPGRRWRGIEGTEAAM
                     RAMEYLQLDPLHIIARSQDITLHSRVLAYTPGRWEDLAYQQRKFFDWGGWLAVRPIDE
                     LPHWRVVMRRERDGDYGDSRIRRMAREHAEAINEMRAILRERGTVSNRDFEMASRTRT
                     QS"
BASE COUNT          858 a         1296 c         1295 g          806 t
ORIGIN      
        1 tattcatgcg cgacaataca atggacaagt tcccggaagc ggagcggcag cgcatgcagc
       61 gagtacacca gtatggcgat gagcaaatac aggcgctttt tcgacatgga cagtcattca
      121 aagacaccta cgatgatatg aacttcacgc ctccctatct gtcaaccatc gctgcccgaa
      181 cactgattgc gtacggcgac cgcgatccgc tcttaccggt gcatattgcg ctggaacaat
      241 acgcggctat tcctcattcg tatctctggg ttgttccgaa tggaggtcat gttcccgtct
      301 tctcagggat atccttcatg gaagccgtgc tgcccttctt gcgtggcgac tgggaacagg
      361 agtaggcatc acatgctcgt ggcatacgga cccggcgacc agccagtcgt ggcggacgag
      421 acaccgttgg agcagttgcg gcagttgtcg caagagcggc tgctgatctg tcccaactgc
      481 cggggcatcg tgcatgtgcg cggcgggccg gagaagcgaa tacaactgca cttcgcccat
      541 caacgcggcg agtgcgcgtg gagtaccgag gccgaatcgg tgcggcacat gcgcgggaaa
      601 atcgtgctgg cgacgtggct gcgcgcgcag tttccccagg ccagcgtgac gctggaggag
      661 cgcctgcccg gctcgaaccg tatcgccgat atcttcatgc agcatcccga cggacaccgc
      721 caggcgctcg aattccagtg cgcgccgctc gacttagacg aatggcagcg ccgtcaccag
      781 gcgtaccgcg ctgcgggcat ttgcgatacc tggatcattg gcgagaaccg tcgcgagaaa
      841 caggaagcgt tcctcgaggc catcctggga acggcgcgcg aaatcctctt tcttgatccg
      901 ctcgccgacg agccgcgtac ctggctgcgc tggcccgcca cacgagccat gatccaggcg
      961 tggcagcgcg aagctaccct tcgtccatcc ttcgagggct gggtaggccg cctgggcagc
     1021 ggcgtaacgc tggtcggcgc gctcatgaca gtacagatcg accgccaggg ccttttgcta
     1081 caccccaggc gcacagccct gcttgcccgt ctcagcctgt tagagcgcat gaccggcgag
     1141 ccggctatcg atgaggccgc cctggtggaa tacctggagc cagcgattgg cgcgcaagcc
     1201 atacagcgcg tcatcctccc tctcttgcgc gcctacaccc gcgatcctga tctgttgcgc
     1261 cgttacaact atggacgcgg ccagcacgga cagccgcctg gcgaggctga cctgctgcgc
     1321 gtgcagcggg cgcgcgcctg gctggaagca ctggcgcagc aggattacga cgctgaacgc
     1381 ctgcgagcgc tggcgaggga tatccctttc gtgggtccgt atgcggcgct cgcaggatac
     1441 ctggagatgt tagtagaact ctaatactta taaaaatgct gctttgtctc ttcctctggc
     1501 gactgctcct tgaagaaggc cgactcgctg cgcttgacgg cgatgtagga tgttgccagc
     1561 agggggccga gcgcgccagt cagcacctcg tcgcgttcca gttcggcgac ggcctcgtcc
     1621 agcgtggtcg gtaggcgctg gatgcctctt tgctcgcgtt cggcgtcgct gtagttgccg
     1681 gggtcgattt gctgtgggtc gccggggtcg atcttgttgg cgatgccgtc cagcccggcg
     1741 gcgatcagcg cgccgatggc caggtagggg ttgccgctgt gatcggcagg tttgagttcc
     1801 aggttgatcg acgtagcttc gcggccccag aagaccgacg gtacgcgaat agcgccctcg
     1861 cggttatcaa aaccatacac agcgtaggcc gaactccaga aatgtggcag caggcggcgg
     1921 taggaattgg ggctgccgca ggtcagagca agcagtccgc gaatgtggcg cagcacaccg
     1981 ccggtgaaat acttgcccgt ctcgctcaac ccgccgcgct ggttggcgtc gtagaacagg
     2041 tttctcccgt tgggaccgct attgtcccac aggctgagat ggatgtgcgc gccgctgccc
     2101 gcctggtcaa gaaaaggctt gggggcgaag ctggcgagca gctcaaagtt gcgcgccaca
     2161 ccgcgcaccg tttcgcgcac caggcaagcg ttgtctgcag cccgcagcgc gtccgcgtgg
     2221 cgaatcgaaa gttcctgctg ccccggccca agttcggtat ggaacagttc gatcgagaca
     2281 ccctggctct ccaatgcctc caaaatagag tcgattacct cggcctggtc atccaggccg
     2341 atgctgctgt agcacaggct gcgatcggca gggacatact tcccgttctc ctcgcgcgcc
     2401 aggtaaaatt cgtgttcgat ggcggcctcg gcgcgcatcc cctgttccgc caggcgcgct
     2461 cccatgcgct tcaggaaggt gcgtgggcag gcgtcccagg gctggccact ggtcaggatc
     2521 atatcgcaca tcatgctgcc gctgctggga acatagggca gaatcgtgaa ggtgctggga
     2581 tcgggaatga ggcggaattc tccgaccggc cccataccct cgaccgaagc gagcgtttca
     2641 acgcccgtaa acgcctgcat cgccaacgtc tggccgatgc cttccttcat gcgggaatca
     2701 agcttgctgc catgcgtagt tttaccccgg ataatgccgc cgttatcgca atacaggaag
     2761 cgtacgagtc gaagatttgc ctctcgtgcc tgcctcacaa catcttgtgt gttcatcgcg
     2821 tcaaaatctc ctgtctacag aataccggag tacgccgaaa aagaggggtg atgtgtgttt
     2881 gagctagtat atcacattca cagtccctgg ggatggaccc ctcgccatat gatgcttgaa
     2941 agaggtgcgc agtcgatttg ggcaaaaaaa cgtcactctg gaagatgaaa caatcgatat
     3001 gagggttggt tctgaccgct tttggtgata tcatgtcggg aaaagaagaa caagggagga
     3061 aacgttatgg atgaaccccc aatggagacg acgtggcgca ccgcgctgtg gcggcagttc
     3121 ggcgcggcca tcgatatgct tgaaaacgcc ctgctggcct gccccgcctc gctgtggaac
     3181 gagcgcctct ggagcgatca agaacaaccg gattacccaa ccttctggta tctcacctat
     3241 cataccctct tctggctcga cctgtatctc accggcaaaa ccgaaggatt cgctccccct
     3301 gcacccttca ggaacccgtt tgacccaaaa agtgcatttc ccacacaacc ctataccagg
     3361 gatgagctcc acacctatct gaagcatctg cgtcagaagt gccagtcaac gatcactgag
     3421 ttgtcagacg agcaaggaca ccaaccgttc catttcccct gggacattga acatccgatc
     3481 agctatggag agctccagtt gtacaccatg cgccacgtcc aggaacatgc ggcccaactg
     3541 agcctcttcc tcggacagca cggcatcccg gacgatgcgc ttgactgggt cgggcgagcc
     3601 aagggggacc ccgacagctg acgcgagcac gtgcggagtg ggctacccgt cgagccgacg
     3661 cacatcccca aaccgcgtct tccaacggag aaggcaggag tgggttgatg attgcaaccg
     3721 aaggaatcag gaacagctcg agatcggttt cgtgggtggg agtgggcttg cacaaaaacg
     3781 aacaggaggt catgatgctc accatcaaca ccgagactac ccgtcgcttc atcctcggca
     3841 agcagggctt gtggccggga cggcgctggc gaggcatcga gggcaccgag gccgccatgc
     3901 gcgcgatgga atacctgcag ctcgacccgc tgcacatcat cgcgcgcagc caggacatca
     3961 cgctgcacag ccgcgtcctc gcttacacgc cgggcaggtg ggaagacctg gcctatcagc
     4021 agcgtaagtt ctttgactgg gggggctggc tggccgtgcg gccgatagac gagctgccgc
     4081 actggcgcgt ggtcatgcgc cgcgagcgcg atggcgacta tggcgactcg cgcattcgcc
     4141 gcatggcgcg cgaacatgcc gaggccatca acgaaatgcg agccatcctg cgcgagcgcg
     4201 gcaccgtgag caaccgcgac ttcgagatgg cgagccggac acgcacccag agtta
//
LOCUS       DASZAK010001023         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_310071, whole genome shotgun sequence.
ACCESSION   DASZAK010001023 DASZAK010000000
VERSION     DASZAK010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310071"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..141)
                     /locus_tag="VGT44_18035"
     rRNA            complement(<1..141)
                     /locus_tag="VGT44_18035"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(384..773)
                     /locus_tag="VGT44_18040"
     CDS             complement(384..773)
                     /locus_tag="VGT44_18040"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HEV2582764.1"
                     /translation="MSPVVLAALLGWLIALVIFLGFIALLRYIDHRERMALIARGINP
                     NRPRQPRRGVGVLRAGLIIAMVGLALTIGLYPLGYMLPASFTSTPLHVGPWLLPGLIP
                     LAVGGALVISVYLSQDARPPGGDEGEA"
     gene            875..1525
                     /locus_tag="VGT44_18045"
     CDS             875..1525
                     /locus_tag="VGT44_18045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2582765.1"
                     /translation="MREHLLPTSSSLGMPLTSAVSTPVEMDDQQLVLASKQGDQDAFS
                     LLVLRYQRHIFNLVYRMVQDYEEANEITQEAFLAAWQGLPSFRGDARFATWLYRIAYN
                     CALKQLETRKRDRALQAALQAEQALDGEDNQVQADARLDAREQQAVVQEQLSYLPAKY
                     RIVLVLRHLQEMTYEEMAEILSMPVGTIKTHLFRARNLLKERLQALYRDSSTETRG"
     gene            1532..>2018
                     /locus_tag="VGT44_18050"
     CDS             1532..>2018
                     /locus_tag="VGT44_18050"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV2582766.1"
                     /translation="MRCSKAERQLQLYIDHKLPLFQLRPLEMHLAGCAACRDELRMLE
                     TVASSLNASQTVSEPRELHEQIMQRVALSAQLSRATGRSQSKPFSPWRPSLAELLAAI
                     LLATVATLGSILEQPALRAMLPFANGHDSLSIAFLHMVHTLTSLDSSTLSLLLWIIGT
                     AL"
BASE COUNT          449 a          580 c          539 g          450 t
ORIGIN      
        1 ccctcactga tggggtaggt tcccatgtct tactcacccg tccgccactc cctctctcct
       61 tgcgaagaaa gagcgttcga cttgcatgtg ttaggcacgc cgccagcgtt tatcctgagc
      121 caggatcaaa ctcgccatcc aaaatgtata tcgaatctcg tcacgagatt caagtgcatt
      181 gtgaatgagt tatcaccaat tgacagggtc aatttgtatt cgtatatgtc ttatccttac
      241 cactcttcag ttgttcaggt acaccagccg cttccgcgcc tgctcattcc tcagttactt
      301 accgaaggta ggtattactg ttgaatgcaa gtgagatatt accactgcct ttgcgccctg
      361 tcaagtactt ttctcgcgag ttttcaggct tctccctcat ccccaccggg tggacgggcg
      421 tcctggctga ggtacacgct gatcaccagc gcgccgccca cggccagcgg gatcaagcca
      481 ggcagcagcc agggaccaac atgcaggggt gtcgaggtaa acgaggcggg cagcatatag
      541 cccagagggt agaggccaat cgtcagagcc agaccaacca tagcgataat caagccggcg
      601 cgcagcaccc ccacgccgcg acgcggttgt cgcgggcgat ttgggttgat gccgcgcgcg
      661 atgagcgcca tgcgttcgcg atgatcgata tagcgcagca gcgcgataaa accgagaaag
      721 atgaccaggg caattaacca gcccagcagg gctgccagga cgacgggtga catatgattt
      781 cttccctatg caaaagctct attcaatatg gtaagatatt actattggag acattgtttc
      841 attcttgagt ctcctgcctg aaatcagggt agttatgaga gaacatctgt tgccaacttc
      901 atccagcctg ggaatgccgc ttacgagtgc ggtctcaact cctgttgaga tggacgatca
      961 gcaactggtc ctggccagca agcagggaga tcaggatgcc ttttccctgc tggtgttgcg
     1021 ctaccaacgg cacattttca acctggtgta ccgcatggtg caggactacg aagaggccaa
     1081 cgagatcacc caggaggcat tcctggcggc ctggcaggga ctcccatcct ttcgcggtga
     1141 tgcgcgtttt gcgacctggc tctatcgcat cgcctataat tgcgcgctga agcagcttga
     1201 gacgcgcaag cgcgaccggg cgttgcaggc cgcactgcag gctgagcaag cgttagacgg
     1261 cgaagacaac caggtacaag ccgatgcccg gctcgatgcg cgcgagcagc aggcggttgt
     1321 gcaagagcaa ttgtcgtatc ttcccgcgaa atatcgcatc gtgctggttc tgcgccatct
     1381 ccaggaaatg acttacgaag agatggctga aatcctatcg atgccggttg gcactattaa
     1441 aacacatttg ttccgcgcgc gcaatttgct caaagagcgc ttgcaagcgc tctatcgcga
     1501 ctcatcaacg gaaactcgag gttgaataat catgcgctgt agtaaggcag aacggcaact
     1561 gcaactctat atcgatcaca agcttccgct ctttcagctg cgtccgttgg agatgcacct
     1621 ggccggttgc gctgcctgcc gcgatgaact gcgtatgctc gaaacagttg cttcctcgct
     1681 gaacgcatcc cagacggtga gtgaacctcg tgagctgcac gagcaaatca tgcagcgtgt
     1741 ggctctctct gcgcaactgt ccagagcaac gggacgatca cagtcgaagc cgttctcgcc
     1801 ctggcgccca tcgctggcgg aattgctggc ggctattttg ctcgccaccg ttgcgacact
     1861 gggatcaatc ctggaacaac cggcgttacg cgccatgctg cccttcgcca atggacatga
     1921 ctcgctctcc atcgccttcc tgcacatggt acacaccttg acatcgctgg atagcagtac
     1981 cctgagcctg ctcctctgga ttatcgggac agcactgg
//
LOCUS       DASZAK010001024         5503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_417469, whole genome shotgun sequence.
ACCESSION   DASZAK010001024 DASZAK010000000
VERSION     DASZAK010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5503
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417469"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            608..1288
                     /locus_tag="VGT44_18055"
     CDS             608..1288
                     /locus_tag="VGT44_18055"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2582767.1"
                     /translation="MNAILQWNYALFQAINAHAGVYPWLDAVMIFCANTLIFFWPLLL
                     LCVWGVPLRWRKAATSPAKAQQLEIRRAAVLWTALACVLAYGLNLLIEQVIFEPRPFV
                     NHPVHLLLTHPADGSFPSDHAAWSFAVVGMIFFALITMSRSAMLPGKAATASMRRATL
                     LFLLIALVIACLIGFARIFVGLHYPGDILGGAIDGLVGASIATALCRLLNRLTLAILR
                     LASFLRLA"
     gene            1311..2594
                     /locus_tag="VGT44_18060"
     CDS             1311..2594
                     /locus_tag="VGT44_18060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HEV2582768.1"
                     /translation="MSTPEMSTYRRYFPSLHQQINGQPAIFLDNPGGTQVAGQVIEAM
                     VQYFREANANTHGAFATSQRTDEVIARARQGMADFLHAASPSQIVFGPNMTTLTFAFS
                     RAIGKTLLEGDEIIVTVLDHDANVAPWLALQERGVVIRTVDVHLEDVTLDMDDLRSKI
                     NERTKLVAVGYAANSAGTINDVATVIRWAREVGALSWIDAVQFAPHGPIDVQQLDPDF
                     LVCSSYKFFGPHLGILYAKSEHLERFPAYKVRPASNQAPERWETGTQNHEGLAGLVGV
                     LDYLALLGREQSERYQHAFEDSQGRPLYTGRPRDLKIAMQALMDYERGLTAYLLAGLR
                     EIAGIQVYGITNPQQLARRVPTVICNLAGHSPRAVAERLATQAIFAWDGNYYALGIME
                     RLGIEEQGGALRLGMAHYNTVEEIDRTLSCLEQIR"
     gene            2620..3555
                     /locus_tag="VGT44_18065"
     CDS             2620..3555
                     /locus_tag="VGT44_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2582769.1"
                     /translation="MLRFLVTRLIGLVFVLFSVSFVTFIMGYFAPGDPIRALLGTHFT
                     PEIYNALKHSYGLDLPWYQQYLNFLNSMLHGSFGLSFEYQGRSAWNVLSEGLPYSIEL
                     GLEVLAATILFGVPSGIISALRANTGADTALTTLAIALYSVPDIAFIISFQLLMVVLY
                     QNNLPNLPIAGWDTWQSRLAPVLITATTGYGYFARLTRTSVIEALGQDFVRTARSKGL
                     RERIVIYRHTLRYACIPLLTVIGPSLGFVVTGVFITEQFFNIPGVSSITLGAIFQRDY
                     PVIQATVILTSASVVIFNALTDITYALADPRIRIE"
     gene            3722..4945
                     /locus_tag="VGT44_18070"
     CDS             3722..4945
                     /locus_tag="VGT44_18070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEV2582770.1"
                     /translation="MASNIENMNTSSEPDNSNLLKRLMEGLFNSSKRPKTPGEFGLAM
                     LKTVPSGLVVIRSFMKEVNWRQAQGDVLKGLDNTIVIVGQPNSGKSTLFNTLKGAQLS
                     PVSAVEGTTRSLVRSTFGPFILIDTPGHLPELMQNGIDEATVVVLLIDATKGLQDKDK
                     ELYSAITTAKKTTIVAVNKVDMLNSVAEGDRAANEVAVALGVAGVIPIAGRTGQNVAE
                     ELIPALIEASPEAALVIGRELPAYRRAAAQRIIRNSTLVSLAAGMEPIPLVDIPILLG
                     TQMRLVLRIAALYGEPMDSADAMKHAKELIATAASGAGMRILAEQAAKAVPFGGDFIA
                     GAIAGAATWAIGQVALEYYEGGKQINPGRLRQLYTSFYRRFRRSRTTEELHTYVIEGD
                     DKPLAIEAPKTRKEA"
     gene            4992..>5503
                     /locus_tag="VGT44_18075"
     CDS             4992..>5503
                     /locus_tag="VGT44_18075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582771.1"
                     /translation="MPFPYERYDTLDTPVKLYYPAGQQAFALQIWQHIEIASQLLAHL
                     LSLPTPAMEILITAPEDWDEAPSEDEEDEEDEEDETGNSVIMLPYLTHSTEPPTLVIP
                     TQLDPIIGNDTVKKRAFLMFHELAHLVLENDPRPWSIDHPLWADEWQMQFAALWLAQQ
                     IFHIHGIVQT"
BASE COUNT         1205 a         1581 c         1509 g         1208 t
ORIGIN      
        1 ggcttgttcg cagattctca attggttcgt gaattgacag gagccgaccc cgcgcttccc
       61 tcgtcgggcg ttccgacgcg gtcctctcca gtcgttcgtt ctgcattcca ctcttcagtt
      121 gtcaaggtac cgagatagtc ctgagactat cttaagagca tttgcagtgt aaaccgcatt
      181 tcgctctttt gctttagcac aggtagtatg tcacatttgt ggtgatctgt caagaggtta
      241 ttgatgagtt tttctcttgt ccagaaaact cgtttgttgc aatgcaacaa acatgctgtg
      301 acaggccgac agaagttgga tggcgtgcca tcataaagaa ggcacaacaa aacattgtca
      361 gcttttcttt gctcaggaaa aggttccgtg aggagagcca cattaacgtg gcgtacactg
      421 cgctacacga cagacaggcg cacctaactt taagcaaatc aggtgattga cttttcgtgg
      481 caagggtgat tatgccacag gcttcgacaa ttgtcaagca atttggggaa ttttctccaa
      541 tctacttcac acctgggata gacctcgcta taatacacgt atcccaaagc ataggagaga
      601 gataacaatg aatgccatct tgcaatggaa ttacgcactg tttcaagcga tcaatgctca
      661 tgccggtgtc tatccctggt tggatgccgt catgatcttt tgcgccaata cgctgatttt
      721 cttctggccg ttgctgctgt tatgtgtgtg gggtgttccc ctgcgctggc ggaaagcggc
      781 gacatcaccc gctaaggctc aacaactgga aattcgtcgc gcggcagtgt tgtggacggc
      841 gctggcctgc gtgctggcct atggcttgaa tttactgata gagcaagtga tcttcgaacc
      901 gcgccccttc gtcaatcacc cggttcacct gctgctcacc cacccagcgg atgggtcgtt
      961 ccccagcgac catgcagcct ggtctttcgc ggtcgtcggc atgattttct ttgcgctgat
     1021 cacgatgtcg cgttctgcta tgttacctgg caaggccgcc acggcgtcaa tgcgccgcgc
     1081 gacgctgctg ttcttgttga tcgcgctcgt gatcgcctgc ctgatcggct ttgcccgcat
     1141 tttcgttgga ctgcattatc caggtgatat actgggagga gccatcgatg gcctggtcgg
     1201 cgcaagtatc gccacagcgc tctgtcgcct tttgaaccgg ctcacgcttg cgatcctacg
     1261 cctggcatcg tttctgcgcc tggcctgaca agaaagaggg ttccctgcat atgtcaactc
     1321 cggaaatgtc cacctaccgt cgctacttcc catcgctgca ccagcagatc aacgggcagc
     1381 cagccatctt cctggataac cctggaggaa cgcaggtagc cgggcaagtc atcgaggcga
     1441 tggtacagta tttccgggag gcaaatgcca acacgcacgg cgcgtttgcg acgagtcagc
     1501 gcaccgatga agtaatcgcg agggccaggc agggtatggc cgattttctc cacgcggcca
     1561 gtccgagtca gatcgtgttc ggaccaaata tgacgaccct gacgttcgcg tttagccgag
     1621 cgatcggcaa gacgctgctc gaaggcgatg aaattatcgt gacggtcctc gaccatgacg
     1681 ccaatgtcgc cccctggctg gcattgcagg aacgcggcgt cgtcattcgc accgtggacg
     1741 tgcatcttga ggatgttacc ctcgacatgg acgacctgcg cagtaagatc aacgagcgca
     1801 caaagctggt ggccgttggt tacgcggcca attcggcagg caccatcaac gatgtagcga
     1861 cggtgatccg ttgggcgcgc gaggtcggcg cgctgagctg gatcgacgcg gtacagttcg
     1921 cgcctcatgg tcctatcgat gtacaacaac tcgatcctga tttcctcgtc tgctcatcct
     1981 acaaattttt tgggccgcat ctcggtatcc tctacgccaa aagcgaacac ctggagcgtt
     2041 tccccgccta caaggtgcgc cctgccagca atcaagcgcc cgaacggtgg gagaccggca
     2101 cgcagaacca cgaagggctg gccggactgg tcggcgttct tgactatctg gcgctgctgg
     2161 gcagagagca gagcgaacgc taccagcatg ccttcgaaga tagccagggt cgaccgctct
     2221 acaccggtag gccgcgcgat ctgaagatcg caatgcaggc gctgatggat tacgagcgcg
     2281 gcctgaccgc ttacttgctg gcgggcttgc gcgaaatcgc gggtatccag gtatacggca
     2341 tcaccaaccc gcagcaactc gcccggcgtg tgccgaccgt gatctgcaac ctcgcgggtc
     2401 actccccgcg cgcggtcgcc gagcgactgg caacccaggc tattttcgcc tgggatggca
     2461 actactacgc gctcggcatt atggagcgcc tgggaataga agagcaaggc ggcgcgctgc
     2521 gcctgggcat ggcgcattac aacactgttg aggagatcga ccgcacgctg agctgcctcg
     2581 aacagatacg ctagctatgc aagacgaagg ggtgccacct tgttacgttt tcttgtcaca
     2641 cggctgatcg ggctggtatt tgtgctgttc agcgtcagtt tcgtcacatt tatcatggga
     2701 tatttcgcgc caggcgatcc catcagggcc ttgctgggaa cacattttac acccgaaatc
     2761 tacaacgctc taaagcactc ttatgggctg gatctaccct ggtaccagca atacttgaat
     2821 ttcttaaata gcatgttgca cggcagtttt ggcttatcat tcgaatatca gggccgttcg
     2881 gcatggaatg tcttaagcga gggtctgccc tattcaattg aactgggatt ggaggtgctg
     2941 gcagcaacca tcctctttgg ggtgccttcc gggattatct ccgccctgcg cgccaatact
     3001 ggggccgata ctgcgctgac aacgctggcg attgcgctgt attccgttcc tgatatcgcc
     3061 ttcattatca gctttcaatt gctgatggtt gtcctctacc agaataatct accaaacctg
     3121 cccatcgcgg gatgggatac atggcagtcg cgtctcgccc cggtactgat taccgcgaca
     3181 acgggatatg gctacttcgc gcgcctgacg cgcacgtctg taatcgaggc gctgggacag
     3241 gattttgtgc gtacggcccg ctcgaaaggc ttgcgagaac gcatcgtgat ttaccgccat
     3301 acgctgcgct acgcctgtat cccgctgctc accgtcattg gcccctcgct cggctttgtg
     3361 gtgacgggcg tctttatcac cgagcagttc ttcaacatcc caggggtcag ttccatcacc
     3421 ctgggcgcca ttttccagcg tgactatccc gtaatccagg caacagtcat cctgaccagc
     3481 gcatcggtag tgatcttcaa tgcgctcacc gacatcactt acgccctcgc cgacccgcgt
     3541 atccgtatcg agtaataacc actcagcgcc gcgaaacgta tacttttgta agagcgagca
     3601 ttccgtaaag gcgcaaggaa atgcgctcct acatggcatt tgcttatctc catttgcagg
     3661 tagtacaatc agtgcaggtc tccggcactc aggccggacc cgttatggaa ggaaacgcag
     3721 cgtggccagc aatatagaga atatgaatac ctcaagcgaa ccggataata gcaacctcct
     3781 caaaagactt atggagggac tcttcaatag cagcaaacgc ccgaaaacgc ctggagagtt
     3841 cggacttgcg atgctcaaga ctgtaccttc cggccttgtc gtgatccgca gctttatgaa
     3901 agaggtcaac tggcgacagg cgcagggcga cgtgcttaag ggattggaca acacgattgt
     3961 gattgtcggg cagccgaatt cgggcaaaag tacgctattc aatactctca agggcgcaca
     4021 gctctccccg gtctcagccg tcgaaggcac gacacgctcc ctggtgcgct ctacttttgg
     4081 gccgttcatc ctgatcgata cgccgggtca tctaccggaa ttaatgcaga atggtatcga
     4141 cgaggcaacc gtcgtcgtgc tgctgataga cgccacgaag ggtttgcagg acaaggacaa
     4201 agaactctac agcgcgatta ctaccgcaaa gaagactacc attgtggccg ttaacaaagt
     4261 agacatgctc aatagcgttg cggaaggcga tcgcgcagcg aacgaggtag cggtggccct
     4321 gggcgtggcg ggcgtcatac ccatcgcggg gcgcaccggc cagaatgttg ccgaagagct
     4381 gattcccgcc ttgatcgaag ccagtccaga ggccgccctg gtgattggcc gcgaacttcc
     4441 cgcttaccgg cgcgcggcgg cccagcgcat catccgcaac tcgacgctgg tcagcctggc
     4501 cgccggcatg gagccgatcc cgctggtaga tatccccatc ttgctcggta cgcagatgcg
     4561 cctggtgctg cgcatcgcgg ccctctacgg cgaaccgatg gatagcgcgg acgccatgaa
     4621 gcacgcgaaa gagttgatcg ccacggcagc cagcggcgcg ggcatgagga ttctggccga
     4681 gcaggcagcc aaagccgtgc cattcggcgg tgacttcatt gcaggcgcta tcgccggcgc
     4741 agctacctgg gccatcgggc aggtcgcgct ggaatactac gagggcggca aacagatcaa
     4801 ccctggtcgg ctgcgccagc tctataccag cttctatcgc cgtttccgcc gctctcgcac
     4861 gacggaggaa ctgcatacat acgtcattga gggagacgat aagccactgg ccatcgaagc
     4921 gcccaaaacg agaaaagaag cctgatagaa gcagagaaag gtaagggcca ttgcgccccg
     4981 attgatgtat tatgcccttt ccctacgaac ggtatgacac actcgatacg ccggtgaaac
     5041 tatattaccc ggcgggacaa caggcgtttg ctctccagat atggcaacac atcgaaatcg
     5101 ccagtcaatt gctcgcgcat ctgctgtccc tgccaacgcc ggcaatggag attctcatca
     5161 cagcgccgga agattgggac gaagctccct ccgaggatga agaggatgaa gaggatgaag
     5221 aggatgaaac cggcaactcg gttatcatgc tgccctacct gacacatagc actgagccgc
     5281 caacgctggt gattccaacg cagctcgatc caatcattgg caacgacacc gtcaaaaaac
     5341 gagcatttct gatgttccat gaactggcgc accttgttct cgaaaacgac ccgcgtccct
     5401 ggtctatcga tcacccactc tgggctgacg agtggcagat gcaattcgcc gcgctctggc
     5461 tcgcccagca aatattccac atacacggca tcgtgcagac cga
//
LOCUS       DASZAK010001025         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_701747, whole genome shotgun sequence.
ACCESSION   DASZAK010001025 DASZAK010000000
VERSION     DASZAK010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701747"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..501)
                     /locus_tag="VGT44_18080"
     CDS             complement(181..501)
                     /locus_tag="VGT44_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582772.1"
                     /translation="MFHYEAMHEERESGSPGSMGGEEFLFNSSAGHKLSQDAPGQAPT
                     VGQRFVLALVSLGMLMGMTTLLTILATARSAPAWEVFPFLFVLTLFTAAVISINVAFS
                     RRVW"
     gene            complement(567..1115)
                     /locus_tag="VGT44_18085"
     CDS             complement(567..1115)
                     /locus_tag="VGT44_18085"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV2582773.1"
                     /translation="MYSKILILAMLRQGPRHGYEIKKDIDRALGGMVVLNNKTLYLAL
                     KHFEEQGAVTGQVIPQEGKPNRHLYELTERGSELLHASLREFPAALASNDAEFFTRVA
                     FFDLLEGPEREAILTTRLAFLEGCLSYLQQMQQLAEGEACAQTAELSLSPAQRVLAFH
                     TRRIHDEYAWIARWLEEMQASR"
BASE COUNT          363 a          479 c          502 g          365 t
ORIGIN      
        1 cagcagtccc accagcaggc gcactgccgt gccgatgggt ccgatctcgc ggtgatggtt
       61 ggccgtctgg tcagtggtgg ggtggtctct cgccttcggg ctggatttgc tcaggcggtt
      121 ggcaaaagtg gtcaccagaa atggctggtt ttcgatcatc ggttcctcct tcgtgatggt
      181 tcaccacact ctgcggctaa aggcgacatt gatactgatc accgctgccg tgaacagcgt
      241 gaggacgaag aggaagggga acacctccca ggcgggagcg ctcctcgcag tcgccaggat
      301 ggtcagcagc gtggtcatac ccatcagcat gcccagcgaa accagggcca gcacgaaacg
      361 ctgaccgacg gtgggagctt ggccgggggc gtcctgggag agcttgtgcc cagcgctgct
      421 gttaaaaagg aattcctcac cgcccataga ccctggagag ccgctctccc gctcctcatg
      481 cattgcttcg tagtgaaaca taccgttgct ccttcttagg cgtgtacatg ccgccaagcg
      541 gaccgggtgc tcgcgacggg acgccttcac cgggaggctt gcatctcttc aagccacctg
      601 gcaatccagg catattcgtc gtggatgcgc cgggtatgaa aggccaggac acgctgcgca
      661 ggggagagcg agagctccgc agtctgcgca catgcctcgc cctctgccag ctgttgcatc
      721 tgctgcagat acgagaggca tccctccaga aaagccaggc gagtggtcaa gatggcctcg
      781 cgctctggcc cctccagcaa gtcgaagaag gcgacccgcg taaaaaactc ggcgtcattg
      841 ctcgcgagcg cggcgggaaa ctctcgcaga gaggcatgca gcaactcgct gccgcgctcg
      901 gtcagttcgt agagatgccg attgggcttg ccttcctggg ggatgacttg cccggtgacg
      961 gcgccttgct cttcaaagtg cttgagagcc agatagagcg tcttgttgtt caggacaacc
     1021 atcccgccca gcgcccggtc gatatccttt ttgatctcgt agccgtgccg tggaccttgt
     1081 cgcagcatgg cgagaatcaa gatcttacta tacatatgat actaccctat atgatactgt
     1141 tacctatata gagataatga taccagagaa gggcgagaat gtcaagggta cctgctgaaa
     1201 agacgtgaag gcgagaagag cgtcagtggc gagtccgcct cttgcgaccc tggaatattc
     1261 catgaactgg ttctcgcggg aggacctcgt ctctcctcca cggcagaagg gaaacgaggt
     1321 gtgtcgaact ggcctattgg tgtgattttg acgaaaaagt atgccgtccc atagaactgc
     1381 ttcgtttgag catgattgtt cttcaggata ctctcaccat gcttgcaatc tcttctcctg
     1441 tccggtatac tataaccgtg gttataaaga aaagcaactt ttgttgcaga gtctacccgt
     1501 aatgcccatg atgccaagcg aggagggagg cgtagatgga tacatcacag acagtcgaag
     1561 cacccgcaac acccacgtca gagcacgcac cctataccct ctggcagctg gtacgctatt
     1621 tcttgcacct gggaacgctt ggctttggtg ggccggtcgc cctggtgggg tatatgcagc
     1681 gcgacctcgt cgagcagcga gcctggatt
//
LOCUS       DASZAK010001026         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_558566, whole genome shotgun sequence.
ACCESSION   DASZAK010001026 DASZAK010000000
VERSION     DASZAK010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558566"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VGT44_18090"
     CDS             <1..612
                     /locus_tag="VGT44_18090"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2582774.1"
                     /translation="LAECYTVVAFDNRGVGQSDKPDIPYTIEMMAEDTAGLLNALGVE
                     QAHLLGTSMGGRIAVALALQHPRLVKSLILVSTMMKSLKGTPMTWSRLRVDLILKLPM
                     IRGSNPYYAVIRQREASRNFDCMDRLNEIHVPTLILHGKKDKSAPYHLAEAMHAGIKG
                     SQMLTFDGGHLFFILRQKQFFEAIADFLDSSKHDLGSSETGGG"
     gene            711..>1755
                     /locus_tag="VGT44_18095"
     CDS             711..>1755
                     /locus_tag="VGT44_18095"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2582775.1"
                     /translation="MTDNQANLDIPGEKSRELLARRQQYVARGVSSTMSVVAAKAEGA
                     IIEDVDGNRFIDFAGGIGVMNIGHSRPEVVQAIAEQAAKFTHTCFAVMMYEPYIELAE
                     RIVNLTPGAFPKKAAFFNSGAEAVENAVKIARYATGKPAIITFENAFHGRTLMTMTMT
                     AKLSYKHHFGPFAPEVYRAPFPYPYRMNLTPQQASDFCLAEIERMIVNEIGADQVAGV
                     IIEPLQGEGGFLPAPAGFLRGLKTLCERYNILFIADEIQSGFCRTGRMFAVEHDGVEP
                     DMLIIAKSMGAGMPISGVIGRAEIMDAPAPGTLGGTYSGNPVSCAAALAVLDLFAQDD
                     YVARSRAIGQKITE"
BASE COUNT          386 a          506 c          502 g          361 t
ORIGIN      
        1 ctcgcggaat gttatacggt cgtcgcgttc gacaaccggg gcgtggggca gagcgacaag
       61 ccggatatcc cctacactat cgaaatgatg gctgaagaca ccgccgggct actcaacgcg
      121 ctgggcgttg aacaggcgca tctgcttggc acatccatgg gagggcgcat agcggtggca
      181 cttgcgctac aacatccccg gctggtcaaa agcctgatcc tcgtctcgac gatgatgaaa
      241 agcctgaaag ggacaccgat gacctggtcg cgtctgcggg tcgacctcat actgaaatta
      301 ccaatgatca gaggctctaa tccgtattat gccgtgatcc ggcaacgtga ggcgtcacgg
      361 aactttgact gtatggaccg cctgaacgag atccacgtgc caaccctcat tctgcatgga
      421 aagaaagaca agagcgctcc ctatcacctg gccgaagcaa tgcatgctgg catcaaggga
      481 tcacaaatgc tcacttttga tggcggccat ttattcttca ttttgaggca gaagcaattt
      541 tttgaggcaa tcgcggattt tttggattcg tcaaaacatg acctgggcag cagcgaaacc
      601 ggcggcggtt gacatggtta gacctccggc aactatggta gaatggttgt atgaccattg
      661 acccgtgagg gtgtgttcta cccgcatttt gcgtacttca ggaggcaagc atgaccgaca
      721 accaggcgaa cttagacatt ccaggggaaa aatcgcgtga gctactggcg cggcgccagc
      781 aatacgtggc gcgcggcgtt tcttcgacca tgtcagtagt tgcggcaaaa gccgaaggag
      841 ccattatcga ggacgtggat ggcaaccgct ttattgactt cgcgggcggc atcggcgtta
      901 tgaatatcgg gcatagtcgc ccggaggtgg tgcaggccat tgcggagcag gccgccaaat
      961 ttacccatac ctgtttcgcc gtcatgatgt acgaaccata catcgagcta gccgagcgca
     1021 tcgtcaacct cactcctggc gcattcccca aaaaagcggc ctttttcaac agtggggccg
     1081 aggccgtcga aaatgccgtg aagatcgcgc gctacgcgac cggcaaaccg gccatcatca
     1141 ccttcgagaa cgcctttcat ggccgcacgt tgatgaccat gacgatgacc gccaagctgt
     1201 cgtacaaaca tcactttggc cccttcgcgc cagaggtcta ccgcgcgcca ttcccctatc
     1261 cctatcgcat gaacctgacg ccacagcaag cctcggactt ctgtcttgcg gaaatcgagc
     1321 gcatgattgt caacgagatc ggcgcggacc aggtggcggg cgtcatcatt gagccgctgc
     1381 agggcgaagg cggctttctc ccggctccgg ctggtttcct gcgcggtctc aagacgcttt
     1441 gcgaacgata caacatcctc tttatcgccg acgagattca atctggcttc tgccgcaccg
     1501 ggcgcatgtt tgcggtagag cacgacggcg ttgagccgga catgctgatt atcgcgaagt
     1561 cgatgggcgc gggaatgccg atcagcggcg taattggccg cgcggaaatc atggacgcgc
     1621 ctgcccccgg cacgctcggc ggcacctaca gcggcaaccc ggtatcctgc gccgccgcgc
     1681 tcgctgtgct tgacctcttt gcccaggatg attatgtcgc gcgttcgcgc gcgattgggc
     1741 aaaagatcac cgagc
//
LOCUS       DASZAK010001027         1628 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_417612, whole genome shotgun sequence.
ACCESSION   DASZAK010001027 DASZAK010000000
VERSION     DASZAK010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417612"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..1141)
                     /locus_tag="VGT44_18100"
     CDS             complement(359..1141)
                     /locus_tag="VGT44_18100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2582776.1"
                     /translation="MNNTEHPRRVALVTGMARGIGEATARLFAARGYRVAAIDMLETG
                     QVVCDDINAAGGECAFYRCNVADEASVQATVAAARERFGRIDALLNIAGVVLVKPLAE
                     TTWDEYRRVVDVNLGGTFLLCKHVLPIMRAQQGGSIVNMASVSGHVGQTDHVVYGSTK
                     GAVLAFTRALAWEVAADHIRVNSISPGSVDTPMLRSDVTLEAQRTGLPFDEIKRLREA
                     EQAFNRWADPAEIAQAVYFLASEAASFITGTDLLVDGGWVAK"
     gene            complement(1284..>1628)
                     /locus_tag="VGT44_18105"
     CDS             complement(1284..>1628)
                     /locus_tag="VGT44_18105"
                     /inference="COORDINATES: protein motif:HMM:NF017652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEV2582777.1"
                     /translation="KGLLPAPYFTTLFFGWPGGSWSPPTLEEYLHRRRLMPEGSIINV
                     SIMGPEQRIILAAAITHGDHVRVGTEDYPYRRGNEAATHDLVADVADLALRLGRSIAT
                     AEETRRILGIAS"
BASE COUNT          344 a          510 c          458 g          316 t
ORIGIN      
        1 aatggttgaa agtgaaagcg aggccagcgc ttcaaagatc aggtagatcg catacgcgct
       61 gaatctcgac gtagaagcat gcataagtta tcctttcctc ggatacgcgc aaaacagtcg
      121 tccgggctgc tgttaagcgc acagcccatg gctctgaaaa aaggataacc tgctaacatc
      181 gatgcacctc tcatagcgtt cttcccccaa caaacatgga ggagcataac aataattcac
      241 caaaaagtat atcacgtaag tggacaatgt gtggccgata aatttgggta tcagggtctg
      301 tgaatatttg ctcttgtaag caaagggcga ccattcacag agctcgattc gcaccttatt
      361 atttcgcgac ccagccgcca tcgaccagca aatcggtgcc agtgatgaat gacgcggcct
      421 cgctggccag gaagtagacc gcctgagcga tttcggcggg gtcagcccag cgattgaagg
      481 cttgctccgc ctcgcgcagg cgcttgatct cgtcgaaagg aagaccggtg cgctgcgcct
      541 ctaaggtgac atccgagcgc agcatgggcg tatcgaccga gccggggctg atcgaattga
      601 cgcgaatgtg gtctgccgca acctcccagg caagcgcgcg tgtgaaggcc agcaccgcgc
      661 ccttcgtcga gccatacacg acgtgatcgg tctgcccgac atggccggac accgatgcca
      721 tattgacgat gctgccgccc tgctgggcgc gcatgatggg caacacatgt ttgcacagca
      781 ggaatgtccc tcccaggttt acatccacga cgcggcggta ttcgtcccag gtcgtctcgg
      841 caagcggctt gaccagcaca acgcccgcga tgttcagcag cgcatcgatg cgcccgaaac
      901 gctcccgcgc tgccgccacc gtcgcctgca cactcgcctc gtcagccacg ttgcagcgat
      961 aaaacgcgca ttcaccgcca gccgcgttga tgtcgtcgca caccacctgt cccgtttcga
     1021 gcatatcgat tgccgccacg cgatacccgc gcgccgcgaa gagccgcgcc gttgcctcgc
     1081 caatgccacg cgccatgccc gtcaccagtg cgacacgccg tggatgctct gtgttattca
     1141 tgctgttagc ctcctaagcg tcgtagggac gcggtgatta acattgagta atcacgcgga
     1201 caacgccgcg caagccccag ggcgaccgca agggtctcta ccccggtggc cgacatattt
     1261 tatcaatctg cgtcctcgcg tccctacgac gcaatcccaa gtatccgccg cgtctcttct
     1321 gccgtcgcga tactgcggcc tagccgcagt gcaaggtcgg ctacatcggc tacaaggtcg
     1381 tgcgtggcgg cttcgttgcc ccgcctgtag gggtaatcct ctgtgccgac gcgcacatgg
     1441 tcgccgtgtg tgatggctgc cgccaggatg atccgctgtt ctggccccat aatgctgacg
     1501 ttgatgatgc tgccctccgg catcaggcgc cggcggtgaa gatattcctc aagcgtcggc
     1561 ggcgaccagg aaccacccgg ccagccgaag aacagggttg tgaaataggg cgcgggcagc
     1621 aagccctt
//
LOCUS       DASZAK010001028         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_558658, whole genome shotgun sequence.
ACCESSION   DASZAK010001028 DASZAK010000000
VERSION     DASZAK010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558658"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="VGT44_18110"
     CDS             complement(<1..1258)
                     /locus_tag="VGT44_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2582778.1"
                     /translation="MSISTPSTEQGHMYQNFIGGEWRNSRSGETFASTNPARTSEVIG
                     RYQRSTAADIEEAVEAAIKAQPGWAALPAPERGEIVLRTALLLEQQKEELATLMTREM
                     GKILRETRGDVQTAIDVAKYVAGEGRRAEGETVPSALPNKFCMTIRQPVGIVGIITPW
                     NFPMAIPAWKTFPALLAGNAVILKPASDTPLLALKLAEALHTAGLPAGVLNVVTGPGG
                     SLGDVLVSHKKVNMISLTGSTEVGRHVAAICGRDLRRCALELGGKNAVIVLEDADLDL
                     AAESVTSGAFGTTGQRCTATSRVIVQKSVQQAFTDRLVAAAEKLCVGDGLNPQIDMGP
                     LVNRGRVKAVQEYAEIGKMEGARLVLGGSPLTGDDYREGAFYEPTIFTDVGPSMRIAT
                     EEIFGPFVSIVPVTTYEEAIKVANSTE"
     gene            complement(1330..1875)
                     /locus_tag="VGT44_18115"
     CDS             complement(1330..1875)
                     /locus_tag="VGT44_18115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582779.1"
                     /translation="MRSISIPRLFVAFILALAFGLTACGNAFQSNTNNGASSATNTPQ
                     ETHAPGQVTPGQVKLVLAKSHYTSNEGITVSIMNGLSSSIYASSYYTNCTLLALEWKT
                     SKGWVSQGRCLSAQSTHVVVLKPGSVTSLQLTPVSGSFKPTANATWQPGTYRVTFYYT
                     TAPDQDSAQGANVQSPDFTIG"
     gene            2022..2540
                     /locus_tag="VGT44_18120"
     CDS             2022..2540
                     /locus_tag="VGT44_18120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2582780.1"
                     /translation="MENQQRFCTTCGQSLAPGTAFCGACGTRVSAPPAAAPGQLYAGA
                     QPGYLLPNAQAPMQGQDDLLLVGLAAGSGARRRGRNAQLRVRRPGSRLRGCGCLLLVL
                     AVLTGPFIGVALTNGRPHLILTYVAGGMVVLFILVLLIGMLATRGGREALSDGCADGC
                     LDAIFGGLLSGG"
BASE COUNT          553 a          727 c          782 g          633 t
ORIGIN      
        1 attcggtcga gttggcaacc ttgatggcct cttcgtatgt tgtgacaggc acaatagaga
       61 caaatgggcc gaatatctct tccgtggcga tacgcatgga tgggccgaca tcggtgaaga
      121 tggtgggttc gtagaatgcc ccttcgcgat agtcatcccc ggtcagaggc gagccgccca
      181 gcacgagcct ggccccctcc attttgccga tttcggcgta ttcttgaact gccttgacac
      241 gccctctgtt cacaagcggt cccatatcga tctgcggatt caagccatca ccgacacaca
      301 gtttttcggc ggcagctacc aggcggtctg tgaatgcctg ttggacagat ttttgcacaa
      361 tcacgcggct cgtcgcggta caacgctggc ccgtcgtgcc gaatgcgccg gaggtcacac
      421 tctcagcggc caggtcaagg tccgcatctt cgagcacgat tacagcgttt ttaccaccca
      481 gttcgagcgc gcagcggcgc aggtcgcgac cgcatattgc cgcgacatgc cgtcccacct
      541 cggtcgagcc tgtcagcgag atcatgttga ccttcttatg actgaccagc acatcgccaa
      601 gcgatccacc cggaccggtg accacattca acacaccagc gggcaatccg gctgtatgaa
      661 gcgcttcagc aagcttgagt gctaatagag gggtatcgct ggccggttta agaatgacgg
      721 cattgcccgc gagtagcgcg ggaaaggtct tccaggcagg aatagccatg ggaaagttcc
      781 agggagtgat gatgccgacg ataccgacag gctgccggat ggtcatgcaa aatttgttgg
      841 gcagcgccga aggaacggtt tcaccctcgg cgcgtcgtcc ctcgcccgcg acatacttgg
      901 cgacatcaat ggcagtctgc acgtctccgc gtgtctctct caaaatcttg cccatttcgc
      961 gggtcattaa cgtggcaagt tcttctttct gttgctcaag cagaagagcc gtacgcagta
     1021 ctatttcgcc acgctcggga gcgggcaacg ctgcccagcc gggttgggcc ttgatcgccg
     1081 cttcgacggc ttcctcgata tccgcggctg tcgatctctg gtaacgtccg atgacctcgg
     1141 aggtgcgcgc gggattggtg ctggcaaatg tctcaccgct tcgtgagttg cgccattcgc
     1201 cgccaatgaa gttctggtac atgtgaccct gctccgttga gggggttgaa attgacatgg
     1261 caaatctcct cgttgagttg tgacatggaa tgtttgacac ccaatttgtg tgcttcaatg
     1321 cacattatat cacccgatgg tgaagtccgg cgattgcaca ttcgcgccct gggcgctatc
     1381 ctggtcagga gccgtagtgt agtaaaaggt gacgcggtag gttcccggct gccaggtagc
     1441 attggcagtg ggtttgaatg agcctgagac gggagtgagt tgcaaggaag tgacggaacc
     1501 tggcttcaat actacaacgt gtgttgactg cgcactgagg cagcgaccct gagagaccca
     1561 tcccttgctg gttttccatt cgagcgcgag caacgtgcag ttggtatagt atgacgaagc
     1621 atagatactg ctggaaaggc cgttcatgat gctgacggta ataccttcgt tcgaagtata
     1681 gtgagatttt gccagcacaa gcttcacctg gcctggagta acctggcctg gcgcgtgagt
     1741 ctcctgcggt gtgtttgtcg ctgaagaagc accattgttc gtatttgatt gaaatgcatt
     1801 cccgcaggcc gtaagaccaa aggccagcgc gagtatgaaa gcgacgaata atcgcggtat
     1861 gctaatagat cgcataatag tcctcctgat ttcagagacg taggattatt tggaaaagtt
     1921 cccttctgga aaaataaacg catgtatggt atgctacctc tcaaaccaaa gcaagcaagg
     1981 gaattcgcgt tctggggacg ctgagtgcca cgaggaggtc aatggaaaat caacagcgat
     2041 tctgcaccac ctgcgggcaa tcgctggcac ctggcactgc tttctgtggc gcctgtggca
     2101 cgcgggtgag cgcgccgcca gcggccgctc caggccagtt gtatgccgga gcgcagcctg
     2161 gctatctact gcccaacgcg caggctccaa tgcaggggca agatgatttg ctcctcgttg
     2221 gcctggccgc tggctcagga gccagacgga gggggcgaaa tgctcagctg cgagtcaggc
     2281 gaccagggtc caggctgcgc gggtgtggct gcctcttgct cgtcctggca gtgcttaccg
     2341 gaccattcat cggcgtggcg ctcacaaacg ggcggccaca cctgatctta acgtatgtgg
     2401 ctggcggaat ggtcgtgctc ttcatcctgg tgcttttgat cgggatgctc gcgaccaggg
     2461 gtggacgaga agctttgtcg gatggctgcg cggatggctg tcttgatgcg atttttggcg
     2521 gcttactcag cggagggtaa tgtccatcag cattcacttc tctctctgct cctcttatca
     2581 cattttcacg tctctgattg agatcctcta gtcttctggc tcatgtgcac agaatcagtg
     2641 agcgccatga caacttttgt tcctactttg gatacgcgag ccgtatatat atggt
//
LOCUS       DASZAK010001029         4590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_525255, whole genome shotgun sequence.
ACCESSION   DASZAK010001029 DASZAK010000000
VERSION     DASZAK010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525255"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1745)
                     /locus_tag="VGT44_18125"
     CDS             complement(<1..1745)
                     /locus_tag="VGT44_18125"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEV2582781.1"
                     /translation="MGQEQRIDDPLKDKQTRLRIHRMRWLIALVALALIVGGTIFWLV
                     DTQGSLYTILPVIIFTVLGVVIGLFQWLFPVGSGAAMLQGQSATMQPALAPQGAAAPP
                     PQIIVHMPVPEHTHPSHTGQLDKITYRGIMGIPPPTDPRAIQPREAKVREIFAKLSEP
                     DVTALVLTGIGGVGKSTLAALVYRYAEAQRLEGHGPFAAPALWLTIDSAVTMADLAGN
                     LFEVFGKPLPDFANLSLQHQAMTLFNVLNTGEQPRLVVFDQFENLLDMQTGHALADRP
                     GVGEWLDAINSQPCACKMLLTSRPWPLGTREYPSTYMQEYIVKGLSVAEGADLLRKLQ
                     IEATDAELRRAVEQCQGHAFSLTLLASLLRHRHLKLSDFFHEAVYSHLWTGNVARNLL
                     DLIYQQQLNEQQRGLLLAFSIYRKPVQLDAAVALLVEGHEARQAHTAHAALDVLLNQH
                     LLQAWGEGRYRLHAIVASYAQSHFVEHDESANREAVRTAHIRAAEHYTRYAQEHCPVS
                     EKREQVGDVEPFVEAVWQLCQAERWPDAYDLMEREGLFALLKRAGGNAILLELYQNLA
                     PERWQPLPEQRARVF"
     gene            complement(1781..2641)
                     /locus_tag="VGT44_18130"
     CDS             complement(1781..2641)
                     /locus_tag="VGT44_18130"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEV2582782.1"
                     /translation="MFDALNLTWHWSPITLVGLVMLCLLYIFGTRRARRKNPSGTPLL
                     ARHVIAFVVAIVLMAAMLLTPIDMIARTQLFSFHMAQIVILTTLCAPLLLYSTPAWLW
                     QPIFEHPGSKPLARILTQPVVVSVIFNLNFLFWHMPLFFGLALQYGSLYHVEIISIFA
                     ASLLNWWPLIGPRRDLHMMSYPQQMLYAFLDGQPVDIFAFLLVFSFVVIYPVYNVPPQ
                     LGISAFGDQAAGGALLLIPGLVDLVVMSPLFFRWLNQLEQKAKVADERRAVEEAMWDA
                     EEAVEQGSEA"
     gene            2984..4129
                     /locus_tag="VGT44_18135"
     CDS             2984..4129
                     /locus_tag="VGT44_18135"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV2582783.1"
                     /translation="MRIVIAPQSLKGSLTAAETGAAIAQGARAVYPQADIAIVPVADG
                     GEGTVQALVDATGGRIVERVVTGPLGEPVPAFFGILGDGTTAAIEMAASSGLPLVPPA
                     QRDPRITTTFGVGELILAALDLDSRHFIIGIGGSATNDGGAGMAQALGASLTDMRGQP
                     IARGGAALGALEHVALAGMDARLQECTFDVASDVNNPLCGPTGASAVYGPQKGATPDM
                     VAQLDAALAHYAGIIERDLGLSVRDIPGAGAAGGLGAGLLAFFRATLRPGAQIVLEAV
                     HLEEQLRHADLVITAEGQIDAQTAYGKSVGAVAALAKRYGIPVLALAGGLGDHYQQVY
                     ALGIDAIAVLPSRPITLDYAMQHAAELASDAIERACKLLQLGATLSI"
BASE COUNT          982 a         1348 c         1409 g          851 t
ORIGIN      
        1 tggaaaacac gtgcgcgctg ttcgggcaag ggctgccagc gttccggcgc aaggttttga
       61 tacaattcca gcagaatcgc gttgccgccc gcgcgcttga gcagggcgaa cagcccttcg
      121 cgttccatca agtcgtaggc atcgggccag cgctcggcct ggcacaactg ccagacggct
      181 tcgacgaacg gttcgacatc gccgacctgc tcgcgctttt cgctcacggg acaatgctcc
      241 tgggcatagc gcgtataatg ttctgccgcg cgtatatgcg ctgtccgtac tgcttcgcgg
      301 ttggccgact cgtcgtgttc gacaaagtgg gattgcgcgt agctggcgac gatggcatgc
      361 agccggtagc gaccttctcc ccacgcctgg agcagatgct ggttcagcag cacatccagc
      421 gcggcatggg ctgtgtgcgc ctggcgcgcc tcgtgacctt ctaccagcag cgcgacggcg
      481 gcatccaact gcaccggctt gcgatagatg gagaaggcca gcagcaagcc gcgctgttgc
      541 tcgttcaact gctgttgata gatcaagtcg agcaggttac gtgccacgtt gcccgtccac
      601 agatgcgaat agaccgcctc gtgaaagaag tcggacagct tcaggtggcg atggcgcagc
      661 agcgaggcca gcaaggtcag cgagaaggcg tgaccctggc actgttcaac ggcgcgccgc
      721 agttctgcat cggtcgcctc gatctgcagc ttgcgcagca gatcagcgcc ttcggcgacc
      781 gacaggccct tgacgatata ttcctgcata taagtagatg gatattcgcg cgtcccaagc
      841 ggccaggggc ggctggtgag cagcatttta caggcgcagg gctggctatt gatggcatcc
      901 aaccattccc cgacgccggg gcggtcggcc agcgcgtggc cggtctgcat atccagcagg
      961 ttctcgaact gatcaaagac gaccaggcgc ggctgctcgc cggtattcag cacattgaac
     1021 agcgtcatag cctggtgttg cagcgaaagg ttggcaaagt cgggcagggg tttgccgaat
     1081 acctcgaaca ggttgccggc caggtcggcc atcgtcaccg ccgaatcgat ggtgagccac
     1141 agcgccgggg cggcaaacgg accatgccct tctaggcgct gcgcctccgc atagcgatag
     1201 acgagagccg ccagggtcga tttaccaacg ccgccgatgc ccgtcaggac gagcgcagtc
     1261 acatccggct cgcttagctt cgcgaaaatc tcccgcacct tcgcttctcg cggctggatg
     1321 gcgcgcgggt cggtcggtgg tggaatgccc atgatgccac gataggtgat tttgtcgagc
     1381 tgccccgtat gcgaaggatg ggtatgctct ggcacaggca tatgcacgat gatctgtggc
     1441 ggcggcgcag cagctccctg gggcgccagc gccggttgca tcgtggctga ctggccttgc
     1501 agcatagccg cgccggagcc gacggggaac agccactgga acaggccaat caccacgccc
     1561 agcacggtga agatgatgac cggcaggatc gtgtaaagcg acccctgcgt gtccaccagc
     1621 cagaagatcg ttccgcccac gatcaacgcg agcgcaacca gggcgatcag ccagcgcatc
     1681 cggtgaatgc gcagcctggt ctgtttgtct ttgagtggat cgtcgattcg ctgttcctgc
     1741 cccatatatc ccccatttta ccatattacc gtatcgtcta ttacgcttcc gatccttgct
     1801 caacggcctc ttcagcgtcc cacatggcct cttccacagc cctgcgttcg tcggcgactt
     1861 tcgccttttg ctcgagctgg ttgagccagc ggaagaagag cggcgacatg acgacaagat
     1921 cgaccaggcc ggggatgagc aggagcgcgc ctccggcggc ttgatcgccg aaagccgaga
     1981 taccgagctg tggcgggacg ttgtagaccg gatagatcac tacgaaagaa aagaccagca
     2041 ggaaggcaaa aatatcgacc ggctggccgt ccaggaaggc gtagagcatt tgctgtgggt
     2101 aggacatcat atgcaggtcg cggcgcggcc cgatgagcgg ccaccagttg agcagcgagg
     2161 ccgcgaagat actgatgatt tcgacatggt agagcgagcc gtattggagc gccaggccga
     2221 aaaagagggg catatgccag aacaggaaat tcaggttgaa gatgaccgag acaacgaccg
     2281 gctgtgtcag gatgcgcgcg agtggcttgc tgccaggatg ctcgaaaatc ggctgccaga
     2341 gccaggcagg ggtagaatat agcaacaggg gagcgcaaag cgtcgtcagg atcacgatct
     2401 gagccatgtg gaaggagaag agctgcgtcc tggcaatcat gtcgatgggc gtcaacaaca
     2461 tcgccgccat cagcacaatg gcgacaacga aagcgatgac atggcgcgcc agaagcggcg
     2521 tccccgacgg gttcttgcgg cgcgcccggc gtgtgccaaa aatatagagc aggcagagca
     2581 tcaccaggcc gaccagcgtg atgggactcc aatgccaggt cagattcaaa gcgtcaaaca
     2641 tggcacatct cgtgtttcta gcaaatacgg cttttgttca ctgctagtat agtgcagggc
     2701 ggctgatgtt gcaaccgttg caaatgccgc aggtattcgt tattttttgc acatagagta
     2761 agtgttctag agggcgacca caagggtacg cccttaccat gactacgaaa cggctctcgt
     2821 gttgcagtcg gacgggtcat ggtaagggcg tacccttgtg gtcgccctct gaacttcatc
     2881 gatacaaaaa ttttagcgga catcttcttg tgaaagtcaa tatgacctgg tctgatataa
     2941 tacgtcaaca gtacagtgtc tcattacaaa atggaggcgt cccatgcgta tcgtcattgc
     3001 gccccaatcg ctcaaaggaa gtctcacggc agccgagact ggcgcggcca ttgcgcaagg
     3061 cgcgcgcgcg gtctacccgc aggccgacat cgccatcgtg ccggtcgcgg atggcggcga
     3121 gggaaccgta caggcgctgg tcgatgccac cggcgggcgc atcgtggagc gggtcgtaac
     3181 ggggccgctg ggtgagccgg tgccggcctt cttcggcatc ctgggcgatg ggaccaccgc
     3241 cgcgattgag atggccgcca gttccggcct gccgctcgtg ccgcccgcac agcgcgaccc
     3301 gcgcatcacg accacctttg gcgtgggcga attaatcctg gccgcgctcg acctggacag
     3361 tcgccatttc atcatcggca tcggaggcag cgctaccaac gatggcggcg cgggcatggc
     3421 gcaggcgctc ggcgcatcgc tgacagatat gcgcgggcag cccatcgcgc gaggcggcgc
     3481 ggcgctgggg gcgctggagc atgtcgccct cgcgggcatg gacgcccgct tgcaggaatg
     3541 cacattcgat gttgctagcg atgtcaacaa cccgctctgc ggtccaacgg gcgcgtcggc
     3601 ggtctacggg ccgcagaaag gcgcgacgcc tgatatggtg gcacagctcg acgcggccct
     3661 ggcacactac gccgggatca tcgagcgcga cctgggtctg tccgtgcgcg atatccccgg
     3721 agcaggcgcg gcaggcggac tgggcgcggg gctgctggcc ttctttcgcg cgacgctacg
     3781 ccccggcgcg cagatcgtcc tcgaagccgt gcatctcgaa gagcagcttc gccatgccga
     3841 tctcgtcatc actgccgagg ggcaaattga cgcgcagacc gcctacggca agagcgtcgg
     3901 agcggtcgcg gccctggcaa aacgctacgg catccccgtc ctggcgctgg caggtggcct
     3961 gggcgatcac taccagcagg tctacgccct gggcatcgac gccattgccg tgctgccctc
     4021 acgccccatc acactggact acgccatgca acacgcggct gagctggcca gcgacgcaat
     4081 cgaaagggcc tgcaaactac ttcaacttgg agccactttg agcatctaag agatgccgga
     4141 atattcctcc acaaaaagaa gttatctata ggtaaggtac ttggggaacc ctaagcgaaa
     4201 cctaagtata tcttaatctc aaagtgtatc aaaggcgtat cgcttcgcgt atacatagca
     4261 aaggctgtag tagtcttttc gcgatgagcc tgcaaagcct tcttaccgcg cccgcttcaa
     4321 aatgaggagt atcattatat gcaagagcat ttgtatgcaa cagataatgc agactttgaa
     4381 caactgtgtg ccttaggctt ttccgaagac gaagccaaac gcctaatcca tatgagagac
     4441 cacatcattg agcagataga gtatcgtgaa atgcaggagg agcaacaccg cctgggcttc
     4501 attcgctggc tgatcgaaca caaccgcctt caaagataaa acgatcacca acaagaaacg
     4561 gctcggcgag ccaaaaactc gccgagccgt
//
LOCUS       DASZAK010001030         1782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_595567, whole genome shotgun sequence.
ACCESSION   DASZAK010001030 DASZAK010000000
VERSION     DASZAK010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595567"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VGT44_18140"
     CDS             <1..829
                     /locus_tag="VGT44_18140"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV2582784.1"
                     /translation="RKHLETHLYHTGEFAQKAAITERTLRYYDKVGLLTPSGYTEAGY
                     RLYTEADLLRLQQILALKFLGFSLDEVKACLRIGPTALQESLAQQKAMMQERREQLDT
                     IIQAIDETEKLLRINQQGWDSIVHVIRVIQMTQNNDWRQKYLTDEQIKKMGEISQQAY
                     TDEDRQKLAVWGKNWSEEDQRRASQQWSEVITELQRLSTSGEQPGSPAAQALAQRWSE
                     LIAQFTHGDPAISKGLGKWYDNLREMPVGDRPIPMPFDEQGGAFLKEAMRIYLEEQK"
     gene            829..1716
                     /locus_tag="VGT44_18145"
     CDS             829..1716
                     /locus_tag="VGT44_18145"
                     /inference="COORDINATES: protein motif:HMM:NF021537.4"
                     /note="predicted Zn-dependent protease with a strongly
                     conserved HExxH motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HEV2582785.1"
                     /translation="MRLHIIDVESIYRRLLATSNAEERESIYRQELLAPFEGMMRFFG
                     SDPLALAKIWTLYTPEEFANETRPAIEAMVDQMAVANAWHRCATALQRGVAAFAPYAD
                     HIPLDTINCALVLLDARRANPIDRGYAGFGGIPGYVMVTYSQANDYTLSRVEGASVHE
                     LNHTVRFKVVPFIPTHVTVADYIIAEGLAESFAAELYGEGVVGYYVTDFSEDELATAK
                     QVIGDALDVSGFDAVRGYIFGDALADHMGLPKAGVPNFAGYVMGYRAVRQYLQRTGKS
                     VAEATFLPSREIIAESGFF"
BASE COUNT          428 a          552 c          488 g          314 t
ORIGIN      
        1 gaggaagcac ctggaaacac atctctacca taccggcgag ttcgcgcaaa aagccgccat
       61 caccgagcgc acgctgcgct actacgacaa ggttggcctg cttacgcctt ccggctatac
      121 agaggcgggc taccggctct acaccgaagc ggatttgctg cgactccagc aaatactcgc
      181 gctcaaattt ctcggcttct cacttgatga agtcaaggca tgcctgcgca tcgggccgac
      241 cgcgctgcaa gaatcgcttg cccagcagaa ggctatgatg caagagcgac gcgagcaact
      301 ggataccatt atccaggcca tcgacgagac ggaaaaactg ctgcgcatca accagcaagg
      361 gtgggattcg attgttcacg tgatccgggt gattcaaatg acacaaaaca atgattggcg
      421 gcagaaatac ctcaccgacg agcaaatcaa gaagatgggg gaaatcagcc agcaagccta
      481 taccgatgaa gaccgccaaa aactggccgt atggggcaaa aactggagcg aggaagacca
      541 gcgccgcgcc agccagcaat ggagcgaggt catcacggaa ctgcagcgcc tctcgacgag
      601 tggcgagcag cccggcagtc cggcagcgca ggctctcgcg cagcgctggt ctgaactgat
      661 cgctcagttc acgcacggcg accccgccat ctcaaaaggg ctgggcaaat ggtacgacaa
      721 tctacgcgag atgcccgtcg gcgacaggcc gatccccatg cccttcgacg agcaaggtgg
      781 agcgttcctc aaagaggcta tgcgcatcta cctcgaggag cagaaataat gcgactacat
      841 atcatcgacg ttgaatccat ctaccggcga ttgctcgcca ccagcaatgc cgaggagcgt
      901 gaatccatct atcgccagga actccttgcg ccttttgaag gcatgatgcg tttctttggc
      961 agcgatcctc tggcgctcgc aaagatatgg accctgtaca cgccggaaga atttgccaac
     1021 gaaacacgcc ccgcaatcga ggcgatggtc gatcaaatgg cggtagcgaa cgcctggcac
     1081 aggtgcgcca ccgccctcca acgaggcgta gccgccttcg cgccctacgc agaccatatc
     1141 ccgctggaca caatcaactg cgcattggtc ctgctcgatg ccaggcgcgc caacccgatc
     1201 gaccggggct atgccggctt tggaggcatt ccaggatacg tgatggtcac gtacagccag
     1261 gccaacgact acaccctgtc gcgagtggaa ggagcatcgg tgcatgaact gaaccacact
     1321 gtgcgcttca aagtcgtacc cttcattccc acacacgtca ccgttgccga ttacatcatc
     1381 gccgaaggcc tggctgaatc cttcgccgcg gaactctacg gagagggggt cgtcggctac
     1441 tacgtcaccg atttcagcga agacgaactg gctactgcaa agcaggtcat tggcgacgcg
     1501 ctcgatgtga gcggcttcga cgccgtgcgc ggatacattt tcggcgatgc ccttgccgac
     1561 cacatgggcc tgcccaaagc tggagtgccg aacttcgcgg ggtacgtcat gggctaccgt
     1621 gctgtccgtc aatacctcca gcgcacaggc aaatcggtgg ccgaagccac attcctcccc
     1681 tctcgcgaaa tcatcgccga atccggcttc ttttagcaag agccgatgtg aatgtcacca
     1741 accaccaccg gccctgtcat cctgagcgca gcgaaggatc tc
//
LOCUS       DASZAK010001031         1796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_558826, whole genome shotgun sequence.
ACCESSION   DASZAK010001031 DASZAK010000000
VERSION     DASZAK010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558826"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..1348)
                     /locus_tag="VGT44_18150"
     CDS             complement(32..1348)
                     /locus_tag="VGT44_18150"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEV2582786.1"
                     /translation="MSFLARLSLKNRSLIALATIAILLIGAYVIPMLKQELFPSLDFP
                     AVSVVTVDAGASPTIVEHDVTNPLEQSIQGIAGIQQMTSYSNQGVSIIVVEYNFGTDI
                     NQASQTLLHGLTALQVGQELRMVYSGTTVTKVTLGGTQEDINIQLGTPATTVQGMQNL
                     LIPGPTGNIRLGDIATVTQTYGPPQITHLGGVRTATVSATITSSNIGAVSQDVQQRIN
                     ALHLPAGATVSLGGVTASQSQAFSGLLLALLIAILLVYLVMMATFRSIILPLILLVSI
                     PFAATGSILLLLITHTALGVPALIGLLMLVGIVVTNAIVLLDLVNQYRAKGLDARAAV
                     VEGGRRRLRPILMTAIATILALLPMALGLSKAGVFIAGPLAIVVIGGLTTSTILTLLL
                     VPTLYVIVEDVRGRFDRRRAPAPTPSERGEDLESRRIDEQEREPHE"
     gene            complement(1482..>1796)
                     /locus_tag="VGT44_18155"
     CDS             complement(1482..>1796)
                     /locus_tag="VGT44_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582787.1"
                     /translation="RFSILARDIHENVLDRDTQRVALEQLQNVFVQHVTDLFQQAIDA
                     GEITREIPATVLATMYEGMSISLLRNQYLAIGETERYAAGELAGFVVSVLLKGIAVEI
                     AP"
BASE COUNT          388 a          498 c          542 g          368 t
ORIGIN      
        1 ctgtctgctc ttcgacaaac gaaggagtat gctactcatg tggttctcgt tcctgttcat
       61 ctatcctcct ggattccaga tcctcccctc tttccgacgg cgtgggtgcg ggcgcgcgtc
      121 tccgatcgaa gcgtccccgg acatcctcga cgatcacgta gagcgtcggt accaggagca
      181 gcgtcaggat cgtcgaggtg gtcagtccgc caatgacgac gatggccagt ggcccggcga
      241 tgaagacgcc cgctttactc aggcccagcg ccatcggcag cagggcgagg atggtggcga
      301 tggccgtcat caggatcggt cgcagacgcc gccgacctcc ctctaccacg gcggctctgg
      361 catccagacc ctttgcccgg tattgattca ccaggtcgag cagcacgatg gcgttggtca
      421 ccacaatgcc caccagcatc agcaagccaa tcaaggccgg tacgcccagc gcggtatggg
      481 taatcagcag tagcaggatc gagccggtgg ccgcgaatgg gatcgacacc agcaggatga
      541 gcggcaagat gatactgcgg aaggtcgcca tcatcaccag gtagactagc agaatggcga
      601 tgagcagcgc gaggaggaga cccgagaagg cctgggactg gctggcggtc acacctccca
      661 gtgacacggt agcgccggca ggtaggtgaa gcgcgttgat gcgctgctga acatcctggc
      721 tgacggcacc gatattggaa ctcgtgatgg ttgcgctcac ggtcgccgtg cgcaccccgc
      781 ccagatgggt gatctggggc gggccgtagg tctgcgtgac cgttgcgatg tcgcccaacc
      841 ggatattgcc ggtaggaccc ggaatgagca ggttctgcat gccctgcacg gtggtggcag
      901 gcgtgccaag ttggatgttg atatcctcct gcgttccacc aagcgtgact ttggtcacgg
      961 tcgttccact atagaccata cgcagttcct gtccgacctg caatgcggtc aggccatgca
     1021 gcagcgtctg gctggcctgg ttgatgtcgg tgccgaagtt atactcgacg acaataatcg
     1081 agaccccctg gttcgagtag gaggtcatct gttgaatacc ggcgatgcct tgaatgcttt
     1141 gctccaaggg gttggtgaca tcatgctcga cgatggtggg cgatgccccg gcatccaccg
     1201 tcacaaccga aacggcggga aaatccaggg aggggaacag ttcctgtttg agcatcggga
     1261 tcacgtaggc gccaatcagt aaaatggcga tcgtggctaa cgcgatgaga ctccgatttt
     1321 tgaggctgag cctcgcaaga aatgacatga cgaactcctc tatacgatca ttgattctgt
     1381 tgctatcaat gggtgcagga acttattcat cgtttagtaa gtaaaaccga tggatagcca
     1441 cggtatcata aacgatcaat gtaactctgc cagaaacact ctcagggtgc gatttcgacg
     1501 gcaatgccct tgagcaggac cgagacgaca aagccggcta actccccggc ggcgtagcgc
     1561 tcggtttctc caatcgccag gtattgattg cgcaagagcg aaatgctcat gccctcgtac
     1621 atggtcgcga gtacggtggc cgggatctct cgggtaattt caccggcatc aatggcttgc
     1681 tggaaaaggt cggtcacatg ctggacaaaa acgttctgca gctgttccag cgctacacgc
     1741 tgggtgtcac gatcgagcac attttcgtga atatcccgcg caaggattga gaagcg
//
LOCUS       DASZAK010001032         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_202349, whole genome shotgun sequence.
ACCESSION   DASZAK010001032 DASZAK010000000
VERSION     DASZAK010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202349"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VGT44_18160"
     CDS             <1..366
                     /locus_tag="VGT44_18160"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - DNA methylation on adenine
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEV2582788.1"
                     /translation="VGTQFTLMDYKTAMQNAQPGDLIYCDPPYRHSQSILYGAQSFDL
                     RDLLLVIQRCKARGVYVALSIDGTKRSGNLLCNIPLPKDLFEREAYIDCGRSMLKRFQ
                     MDGQSLEKEVVADRLLLTY"
     gene            complement(373..1257)
                     /locus_tag="VGT44_18165"
     CDS             complement(373..1257)
                     /locus_tag="VGT44_18165"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2582789.1"
                     /translation="MKKTPKTPQLANQPDIQEPQTSALPPVSPIASAASAVEQNVKPV
                     VAEAQRVAAETSVSGRRAVSRGVIFLSFYLWLLSGAIMLSLLARYTQFFPGDRTIARN
                     LQKNRNSWLRRFMIAISEPGFPRIGIPLTFSIAGIFWALRFRLEAIFLLLSSSSTILN
                     WIVKRLIKRPRPTSELVTVARVINEPSFPSGHVMHYMNWFGLLTYFLATNWRSGRLRN
                     TLIAICTSLILLVGPSRIYLGAHWPSDVLAGYIYGGLWFSGVMVLYLRVKAWVHPAQG
                     KTPEAMKPLQQPGDEETG"
     gene            complement(1279..>2053)
                     /locus_tag="VGT44_18170"
     CDS             complement(1279..>2053)
                     /locus_tag="VGT44_18170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV2582790.1"
                     /translation="ELVIAAGGDGTIHAVANGLVGTRSILGIISTGTMNNLAHSLNIP
                     DTIPDACTAIATGVTRTIDLGKINDRVFLEVAGIGLEAALFPAAEEIKQPGFLTTVHG
                     AVSGLKTLLGYKSTRLSISVRHRSHPYTALQVTICNAPFYGAHFQIAPQILMDDGLLD
                     VVIYRNFSKLEFLRHARSISQGNRPYQPKIKHLRLQSLRVDAAQPVEIHADGEVIGTT
                     PATVTVLPGALRVRVSGKSAPGLDNTHQAAENALSGKLR"
BASE COUNT          450 a          541 c          609 g          453 t
ORIGIN      
        1 gttggtactc aatttacctt gatggactac aaaacggcca tgcaaaatgc tcagcctgga
       61 gacctgatct attgcgaccc accctatcgt cacagccaga gtatactcta tggggcgcaa
      121 tcgttcgact tgcgcgactt gctcctcgtc atccaacggt gcaaagcgcg cggcgtgtat
      181 gtcgcattga gtattgacgg cacgaaacgc tctgggaatc tcctctgcaa tatcccctta
      241 ccgaaagatt tgtttgaacg cgaagcctac atagactgtg ggcggtctat gctcaagcga
      301 tttcagatgg acggacaatc gcttgaaaaa gaggtggtag ccgatcgact gctgttgacg
      361 tattgaaggc atttaccccg tttcctcatc tccaggctgc tgcaagggct tcatcgcctc
      421 tggagtcttt ccctgcgctg ggtggaccca cgccttaact cgcaggtaga gtaccatcac
      481 gccgctgaac cagaggccgc cgtaaatgta gcctgccagc acgtcgcttg gccagtgtgc
      541 gcccaggtag atacgtgaag gaccgacgag caggatcagg gaggtgcaga tggcgatgag
      601 ggtattgcgg agtcggcctg accgccagtt ggttgcgagg aagtaggtca gcaagccaaa
      661 ccagttcatg tagtgcatga cgtgcccgct ggggaaactt ggctcgttga taacgcgcgc
      721 cacggtaaca agctcgcttg ttgggcgcgg gcgtttaatc aggcgcttta cgatccagtt
      781 gagaatcgtg gaggaactgc tcaacagcag aaagatggcc tccagccgga aacgcagcgc
      841 ccagaagatg ccagcgatgc taaaggtcag tggaataccg atgcgtggaa agcccggctc
      901 ggaaatggca atcatgaagc gacggagcca tgaattgcgg ttcttttgta gattgcgtgc
      961 gatagttctg tcgccgggga agaactgcgt atagcgggca agcagggaga gcatgatggc
     1021 cccggagagc agccagagat agaacgacag gaagatcacg ccacgagaaa cggcgcggcg
     1081 gcccgaaacc gacgtttcag cagccacacg ctgagcttcc gcgaccacag gtttgacgtt
     1141 ttgttcgact gcgctggcgg cgctggcaat gggagaaacg ggcggtagcg cgcttgtttg
     1201 cggctcttga atatctggtt gattggctag ctgtggtgtt ttaggcgttt tcttcacaat
     1261 gcggttcctc cacactcttt aacgaagttt ccctgatagg gcgttctcgg ctgcttgatg
     1321 ggtgttgtct aagcccggcg ctgacttgcc agagacgcgc acacgcaatg cgcccggcag
     1381 cactgtgacg gtggccggcg ttgtgccgat cacctctcca tcggcatgta tctctactgg
     1441 ttgtgctgca tcgacgcgca gcgactgaag gcgcaagtgc ttgattttgg gttgatatgg
     1501 ccgattgccc tggctgatag atctggcatg acgaaggaat tccagcttgc taaagttgcg
     1561 ataaatgacg acatccagca gcccatcatc cataagaatc tgcggagcga tttgaaaatg
     1621 cgcgccataa aaaggcgcat tacaaatcgt cacctgtaag gcagtatagg gatggcttct
     1681 atgcctgacg ctaatgctca agcgcgttga tttataaccg agcagggttt tcaatccact
     1741 aactgcgcca tgtacggtag tcaagaatcc cggctgcttg atctcttcag ccgccgggaa
     1801 cagggccgct tccagaccga tacccgccac ctctagaaac acgcggtcgt tgatctttcc
     1861 caggtcaatc gtgcgcgtca cgccggtggc gatggctgtg caggcgtcgg ggatcgtgtc
     1921 ggggatgttg aggctgtgtg ccaggttgtt catggtgccg gttgaaatga tgcccagaat
     1981 gctgcgcgtg ccaaccagcc cgttcgcgac agcgtggatc gtgccgtcac cgcccgccgc
     2041 tatgaccagc tcc
//
LOCUS       DASZAK010001033         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_131948, whole genome shotgun sequence.
ACCESSION   DASZAK010001033 DASZAK010000000
VERSION     DASZAK010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131948"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..643)
                     /locus_tag="VGT44_18175"
     CDS             complement(92..643)
                     /locus_tag="VGT44_18175"
                     /inference="COORDINATES: protein motif:HMM:NF017913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible PsiE family
                     protein"
                     /protein_id="HEV2582791.1"
                     /translation="MSDHQMSMGEEPREAYSAIKVEERKEEEDVVENAIGKFSGRLLD
                     RGDSIIYAIVGVCFFLGGFIALGYSFWDFGNALVTAKALNPSVFAIAIIQFVSDLLLV
                     LIIMEVLGTVTHYLKSHTTSLRPFLFIGIVSATRGILSVGARLSVGENVDFNHAMIEL
                     GVNGAVILAFGITLKLLGKLVEE"
     gene            complement(722..2746)
                     /locus_tag="VGT44_18180"
     CDS             complement(722..2746)
                     /locus_tag="VGT44_18180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inactive serine/threonine-protein kinase VRK3"
                     /protein_id="HEV2582792.1"
                     /translation="MSVTQLNANDTGLHRSACQRCGSDLPEGAAFCVVCGERLDSEQY
                     LTHLLDQEADMASRYRMTSLVRKRPFVNLYFASDFEQTARQGQARMVAIRDIDCTSLS
                     EAALVKAVKVAQQEYDLLRRSEIPSLASAIDLCYSQKHLYLVANQPPTTPSPASTGDR
                     REERDGRLTTLQDYLQSGQGLPSEQRALEWIAQLCEAVHSLHQRQVVIGDLDPFSVLY
                     CGAMNDAGAARLALLVAWLPPLLRDVLPLSSEPTSSFFSYFVAPEVAQGQVDARSDVY
                     SLGAMLYLLLTGMPPGEATLRNRGRQALLHPPHEINGQVSVRISECVMQALALQPTQR
                     FDSVMAFAAALRDPRYRRSPVRIVEDESDSGDEGGDVETVRIFPLSWKDVERWRAARR
                     HAAPTAPVAPAPQLDPAPVLQAPAIPETPPADVDAPSTVETLLDRDDDLEDRPDEPID
                     FAPEAAWPESPESLAQNTPGWKQRITGILPAISSSLVQTRRVLQAPIAQPGRFQWIAQ
                     SRGRWLDQLKQALLGQQQQVILAAAIVETPLRVLPDQMYTLRIHVVGRNDPSQARRKR
                     SAEDEMNLSRLVCGDTVMIEVRSVLHQSYAYIVQRASVAIPAEGYVAEVTIPMQSLST
                     APIGRRDRLHIFFLDEQRHPLYEKPFAVEIFVSHHVKPGNEGHHVLTIPL"
     gene            3078..3617
                     /locus_tag="VGT44_18185"
     CDS             3078..3617
                     /locus_tag="VGT44_18185"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEV2582793.1"
                     /translation="MDLPTVLFWIIAVILVGSALLVVGLRNIVHSALALVVLFAMAAG
                     IYLLLNAEFIAIVQILIYAGAVTILILFALMLTRTANLSTDSNPNNRQWWLAAIISAV
                     VGAAIIFATTLGQFSTARSGSGTPYGSSACALPPNNVVYIGQLLYSPTCYSYVLPFEI
                     ASLVLLVAIVGAIVIGKED"
     gene            3622..>3857
                     /gene="nuoK"
                     /locus_tag="VGT44_18190"
     CDS             3622..>3857
                     /gene="nuoK"
                     /locus_tag="VGT44_18190"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEV2582794.1"
                     /translation="MGLTLNHFLILGALLFCIGLYGAIAKRNAIAVLMGIEIMLNAVN
                     ITMIGFSYFNTVKPYATYLTGQVFAIFIITVAAA"
BASE COUNT          842 a         1125 c         1029 g          861 t
ORIGIN      
        1 ggtggccgat aaatcggcga tgtgcgcgat aaatcggcac ctacagttgt aacccattaa
       61 ttatttaatt ttcaaaatcg caccctaacg gctactcctc gacgagcttc cccagcagct
      121 tgagcgtgat gccgaatgcg agaatcactg ctccgttgac gcccagttcg atcatagcgt
      181 ggttgaagtc tacattctct ccaaccgaga gcctggcacc aacggagaga atgcctcgtg
      241 tggcagaaac gatgccgata aagaggaatg gtcgcagcga cgttgtatgc gatttcaagt
      301 aatgtgtgac tgttccaagt acctccataa taatgagtac caggagcaag tctgagacaa
      361 actgaatgat ggcgatggca aagaccgatg ggttcagagc tttcgcggta accagggcgt
      421 tgccgaaatc ccagaagctg taccccagtg cgataaagcc tcccaggaag aaacagacac
      481 ccacaatggc atagatgata gagtcgccgc gatccagtaa ccgccccgaa aatttaccga
      541 tggcgttctc tacaacatcc tcctcctcct tgcgttcttc tactttgatg gcgctgtacg
      601 cttcgcgcgg ttcctcgccc atgctcattt gatggtctga catacgtgtg actccttatc
      661 ggatagatga agacaattcc ggtgtattat acccgacaga agatagtatg tggaagagct
      721 tttacaacgg aatcgtcagc acgtggtggc cttcgttgcc gggtttgacg tgatgggaga
      781 cgaatatctc gacggcgaaa ggcttttcat agagcgggtg gcgttgttca tcaaggaaaa
      841 agatatgcag gcggtcacgg cgtcctatag gagcagtaga gagcgattgc atcgggatgg
      901 tcacttcagc aacatagccc tctgccggaa tggccacgct ggcacgctgc acgatatagg
      961 catagctctg atgcagaacg gagcgcactt cgatcattac cgtatcgccg cataccagcc
     1021 tgctcaggtt catctcatct tcggcagagc gttttcgcct ggcctggctg ggatcattcc
     1081 gccccacaac gtggatgcgc agcgtataca tctggtccgg caagacacgc aatggcgttt
     1141 cgacaatcgc tgccgccaga ataacctgct gctgctgacc cagtagcgcc tgcttcaact
     1201 ggtcgagcca tcttccccga ctctgtgcaa tccattgaaa acgtcctggc tgagcgatcg
     1261 gagcttgcag cacacgcctg gtttgcacca ggctggacga aatagccgga aggatgccgg
     1321 taatacgctg cttccagccc ggcgtatttt gcgccaacga ctccggcgac tctggccatg
     1381 ctgcttctgg cgcaaagtcg attggctcat ctggccgatc ctccaggtcg tcgtccctgt
     1441 ccagtagtgt ttcaacagtg ctcggcgcat ccacatccgc cggcggcgtc tcagggattg
     1501 caggggcctg caacactgga gctgggtcta actgcggcgc tggcgcaacg ggcgcggttg
     1561 gcgcagcatg gcggcgagct gcgcgccagc gctcaacatc cttccatgac agggggaaga
     1621 tgcgcaccgt ctcgacatca ccaccctcat cgcccgaatc actctcgtct tccacaatcc
     1681 taaccggaga tcggcgataa cgcgggtcac gcagagccgc agcgaaggcc atcacgctgt
     1741 caaatcgctg cgtcggctgc aacgcgagcg cctgcatcac gcattcgctg atccgcacgc
     1801 tgacctggcc attgatctcg tgaggcgggt gcagcagcgc ctgccgaccc cggttacgca
     1861 gcgtcgcttc gcccggcggc atgcccgtca acagcaggta aagcattgcg ccgaggctat
     1921 acacatccga ccgagcatca acctgtccct gcgccacctc cggcgcgaca aaatacgaga
     1981 agaagctgct tgtcggctct gacgacagag gcaatacatc gcgcagcagt ggcggcagcc
     2041 acgcgacgag cagcgcaaga cgagccgcgc ctgcatcatt catcgcgccg caatagagta
     2101 cggagaacgg atcgagatca ccgatcacca cctgacgctg gtgcaggcta tgcaccgctt
     2161 cgcacagttg cgcaatccat tcaagcgcgc gctgctcaga cggcagaccc tggccactct
     2221 gcaagtaatc ttgaagcgtg gtcagtcgcc catctctctc ctccctcctg tcgcctgtgc
     2281 tggccgggga tggagtagta ggtggttgat tggccacgag gtacaggtgc ttctgggaat
     2341 aacacagatc aatagcagag gcgagagaag ggatctcgct ccggcgcaaa agatcatatt
     2401 cctgctgcgc cactttgacc gctttgacca gcgcggcctc gctaagcgag gtgcaatcga
     2461 tatcgcggat cgccaccatg cgcgcctgcc cctgcctggc agtttgctcg aaatctgagg
     2521 cgaaatagag gttgacaaat ggccgcttac gcaccaggga ggtcatacga taacgcgatg
     2581 ccatatccgc ttcttgatcg agcaagtgcg tcaaatattg ttcgctatcg agccgctcgc
     2641 cgcaaacaac gcaaaaggcc gccccttctg gaaggtcgct cccacagcgc tggcaggcac
     2701 tgcgatgtag tccggtatca ttcgcgttca gttgtgtgac gctcatatgc taactttcta
     2761 gcaataccat gtttggccgc ctgctaagca ttataacata gttcgatgtc tcgattcata
     2821 tacgtttgca tgtatttctc tacccattca gaaaaacgta catctcttgc cgccaggatg
     2881 tttgttagga gaattacctc atgtatagac aaatgcggcg catgaaattc ttgtcagcac
     2941 ttgtatcccc ttgccagcgc atactcgttc tgatagaatc acgttgtaag tcaataaagc
     3001 gtatagagcg tatgtgcgct gtgcgtgtgc ttttctggga acgggcatga tacaataaaa
     3061 ttggaggttt attttttatg gatttaccca ccgtattgtt ctggatcatt gctgttatcc
     3121 tggtggggtc tgctttactc gtcgtaggat tgcgcaacat tgtgcatagt gccctggctc
     3181 tcgtcgtctt gttcgcgatg gcggcgggca tttatctctt attgaatgcc gagtttatcg
     3241 ccattgtgca aatcttgatc tacgctggag ccgtgaccat cctgatcctg ttcgcgctga
     3301 tgctcacgcg caccgcgaat ctgagtacgg atagcaaccc caacaaccgc caatggtggt
     3361 tagctgccat catcagcgcc gtcgtcggcg cggccatcat cttcgccacc acgctcggcc
     3421 agttctctac cgcgcgcagc ggcagcggga cgccctatgg cagcagcgcg tgcgccttgc
     3481 cgcccaacaa cgtggtctac atcggccagc tcctgtacag cccgacctgc tacagctacg
     3541 tgctgccctt tgaaatagcg tccctggtgc tgctcgtggc catcgtcggc gcgatagtga
     3601 tcggcaagga ggactgaacc gatgggattg acgctcaacc acttcttgat cctcggcgcg
     3661 ctgctcttct gcattggcct ctacggggcc atcgccaagc gcaacgccat tgccgtgctg
     3721 atgggcatcg aaatcatgct gaatgccgtc aacatcacca tgatcggctt ctcgtacttc
     3781 aacacggtca aaccctatgc cacctacctg accgggcaag ttttcgccat cttcatcatc
     3841 accgtcgcgg cggcgga
//
LOCUS       DASZAK010001034         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_239229, whole genome shotgun sequence.
ACCESSION   DASZAK010001034 DASZAK010000000
VERSION     DASZAK010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239229"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..701)
                     /gene="recR"
                     /locus_tag="VGT44_18195"
     CDS             complement(102..701)
                     /gene="recR"
                     /locus_tag="VGT44_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV2582795.1"
                     /translation="MSDIAPPVAALIEELSKLPGVGVKTAQRLTFFILRSPTDQARRL
                     AEAIMRVKESIIYCSRCFNITETDPCPICSNPNRDQEMICVVEEPLDVLALEKTSAYK
                     GLYHVLHGAISPVDGVGPKDLRIDELLQRVRTMHLREIILATNPNFEGEYTANYIQKE
                     LKPFNVRVTGLARGLPMGGDLEYADEGTLGRAIEGRREM"
     gene            complement(713..1009)
                     /locus_tag="VGT44_18200"
     CDS             complement(713..1009)
                     /locus_tag="VGT44_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV2582796.1"
                     /translation="MNMREIQKVQQRLMKMQEELEASQFTGTAGGGAVTITMSGKYEI
                     TAVKIDPEAFNPEDIGELEVMVLAACKAVYEKVSDAQQKMTSSVTGGLKIPGLF"
     gene            complement(1027..>2436)
                     /locus_tag="VGT44_18205"
     CDS             complement(1027..>2436)
                     /locus_tag="VGT44_18205"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582797.1"
                     /translation="ELIARAAAGGMRDALSLLDQAIAYAGSDIALAHVQSMLGVADPR
                     AISTFITSIAEFDGSAVLHLINDLSEAGADLRQVNAQIIEYWRALMLTRAGANAAEIL
                     DLIEDEMKGIKLLAERFILEELTECARIFAQNELTQKNQGTPQLGLELAALDCIEVHR
                     RAQAGQPVRAVAAPVAPVPARPQEQRVALPQQATPERDAPMRAAPAAQNGRSPAQRID
                     RPAQETIAPEPPRQAVSSAPTGGESSGESGRPTLTVLQVARAWENVRKRAKQKSAMLA
                     AYLAYIEVVGIEGTAEQPVVVIRATKATHLKYVKDENRYKELEWALQVEFNLPSCQVR
                     LLSPDQSPTIAEAPRIYSTSVSPLAAPQDSAFREPPMTIEPAEPTMSAPQSTHDGEQD
                     SDASSSLNSYAADAGGLSPGTGLARKHVIRENRQEALSASHESREAKETLEQKARHDP
                     VVQEVMRTFKANIVDIHPK"
BASE COUNT          427 a          738 c          718 g          553 t
ORIGIN      
        1 catgcaggca tgtcaagaaa aacttcagct taccctacac gttaatcgtt aggcaatccg
       61 agaagacctg ccacagagat atcttcgtca ttatacacta cttacatctc cctgcgaccc
      121 tcaatcgctc gccctagcgt cccttcatct gcgtattcca gatcaccgcc catgggcagg
      181 ccgcgcgcca ggccggtaac gcgcacattg aacggcttca gctccttctg gatatagttg
      241 gccgtgtact cgccctcgaa gttgggattt gtagcgagga tgatctcgcg caggtgcatg
      301 gtgcgcacgc gctgcaacag ctcatcgatg cgcaggtctt tcgggccgac gccatccacg
      361 ggcgagattg cgccgtgcag cacgtggtaa agtcccttgt acgcgctcgt cttttcgagg
      421 gccagcacgt caagcggctc ctcgacgaca cagatcatct cctggtcgcg gttcggattg
      481 ctgcagatcg ggcagggatc ggtttcggtg atgttgaagc agcgcgagca gtagatgatg
      541 ctctctttaa cacgcatgat ggcctcggcc aggcgacgcg cctggtcggt cgggctgcgc
      601 aggatgaaga aggtcaggcg ctgcgccgtt ttgacaccga cgcccggcag cttcgagagt
      661 tcttcaatca gcgccgcgac gggtggggca atatcgctca tggtcaggta tcctagaaca
      721 ggccgggaat tttaaggccg ccggtaacgc tgctggtcat cttttgctga gcatccgaaa
      781 ccttctcgta cacggctttg caggcagcca gcaccatcac ctccagctcc ccgatatctt
      841 ccgggttaaa ggcttccggg tcaattttga cggcggtgat ttcatatttg ccgctcatgg
      901 tgatcgtcac agcgccgccg ccagccgtgc cggtgaactg ggatgcttcc agttcttcct
      961 gcatcttcat cagccgctgc tgcactttct ggatttcgcg catgttcata atggacaatc
     1021 tgctccttac tttggatgaa tatcgacgat gttcgcctta aacgttcgca tcacttcctg
     1081 cacgacgggg tcgtgacgcg ccttttgttc gagcgtctct ttggcctctc ttgactcgtg
     1141 tgaagcagat aatgcttcct gtctattctc tcttatcacg tgctttctgg caagtcccgt
     1201 gcctggcgac aagccaccgg catcagcagc atacgagttc agcgaagacg acgcatcgct
     1261 atcttgctcg ccatcatgcg ttgattgagg cgcagacatg gttggctcgg caggttcgat
     1321 cgtcataggc ggctcgcgga atgcggaatc ctgtggcgcg gccaatggcg aaacgctggt
     1381 agaatagatt cgcggcgctt cggcgatggt tggcgactga tctggtgaga gcagacgcac
     1441 ctggcacgag ggcagattga actccacctg cagcgcccat tccagctctt tgtagcggtt
     1501 ttcgtccttc acatatttca aatgcgtcgc ctttgtcgcc cggatcacta ccacaggctg
     1561 ttcagccgtt ccttcgatgc caactacctc aatataggcc aggtacgccg ccagcattgc
     1621 gctcttctgt ttggcgcgtt tgcgcacatt ctcccaggcg cgcgccacct gcaaaacggt
     1681 cagggtcggt cgtccgctct ctccactgct ttcgccgcct gttggggcag aactcaccgc
     1741 ctgccggggt ggctccggcg cgatggtctc ctgggcaggc cggtcaatgc gctgcgcagg
     1801 ggagcggcca ttctgcgccg ctggcgcggc tcgcatcggc gcatcgcgtt ccggtgtcgc
     1861 ctgctgcggt agggcgactc gctgttcctg cggtcgcgca ggcacggggg cgacaggcgc
     1921 ggcgacggcg cgcacaggct ggcccgcttg cgcgcggcga tgcacttcga tgcagtcgag
     1981 ggcggcgagt tccaggccaa gctggggcgt tccctggttc ttctgcgtca gttcgttttg
     2041 cgcgaagatg cgcgcgcatt ccgtcagctc ttcgagtatg aagcgctcgg cgagcagctt
     2101 gatgcccttc atctcatctt caatcaggtc aagaatttcg gcggcattcg cgcctgccct
     2161 ggtgagcatc agcgcgcgcc agtattcgat gatctgcgca ttgacctggc gcaggtccgc
     2221 gccagcttcg gaaaggtcgt tgatgagatg cagcacggcg gagccatcga attcggcaat
     2281 actggtgatg aatgtcgaga tagcgcgcgg gtcggccaca ccgagcatgg actgcacgtg
     2341 ggccagcgcg atgtcgctgc ccgcgtaagc gatggcctgg tcaagcaggc tcaaagcgtc
     2401 gcgcatcccc ccggctgccg ccctggcgat caattc
//
LOCUS       DASZAK010001035        14307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_310536, whole genome shotgun sequence.
ACCESSION   DASZAK010001035 DASZAK010000000
VERSION     DASZAK010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 14307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14307
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310536"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1956)
                     /locus_tag="VGT44_18210"
     CDS             complement(211..1956)
                     /locus_tag="VGT44_18210"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2582798.1"
                     /translation="MGMTNDGLSNWQAEDGGIEILEMTPGQLLDVRAEEMPDREALVY
                     SCYPEFGGALDIRWTYREYRERVDAVARGLMALGLQKGEHIAVWAINLPEWPLLELAA
                     SKAGLVLVTVNPVLRAQEVEYILKQGDVAALFFMARVRDHDCLATVRERVTPGAAHGE
                     VSSEMLPHLRYVSLLGAPPAGLLEQEGWRPALFREMVAAGERVSMEALRERQAAVQPG
                     DPAQILYTSGTTGFPKGAIQTQRAILNNGRIFALRWGIQPGEHVCTAMPFFHAGGCVL
                     GIMATLGIGGTLHPLIAFDPLKALQILSSERCSYFGGVPTMLLAILQHPDFGSFDLSS
                     LKSVVSGGSPVPVYLMEQVKERMGADATIVFGQTEASAAITLTKREDSFELKSSTVGV
                     PMPHGAVRIVDPATGEAVPCGERGELVCRGYLVMSGYYHMPEKTAEAIDSEGWLHSGD
                     LATMNAQGYINIVGRLKDMVIRGGENIFPREVEEFLIRHPRIADVQIVGVPDKFFGEE
                     LLAVVIPQQGETLTEAELREYCKGQISHQKIPRYFQFIESYPLTASGKVQKFVLRQNA
                     VKSLGLEEAAQIKTA"
     gene            complement(2252..2719)
                     /locus_tag="VGT44_18215"
     CDS             complement(2252..2719)
                     /locus_tag="VGT44_18215"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV2582799.1"
                     /translation="MDISSENIVEVINSTRTGSVWEWPDIRLVTAEKDKVVATMPIGP
                     NHRQQVGYLHGGISVTLAESVASLGTVLNIDAANQMAFGLEINANHLRPKRDGQLTAT
                     ATPVHRGRTTHVWDVRITDENDKLVCISRCTVAVVDRPPDTGSPFARNLPSFG"
     gene            2866..3315
                     /locus_tag="VGT44_18220"
     CDS             2866..3315
                     /locus_tag="VGT44_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEV2582800.1"
                     /translation="MFDKSNIGRSFTPFTIEVERSKIREIAQAIGDDNPIYQSTAAAQ
                     AAGYKDIPLYPTSPTSFGFWGNRGMGMQLASTGLNVMRILHGEEEYEYLAPIYPGDTL
                     TGVVTILDGKTRQGQAGSMDIVTTETSYTNQHGKPVLKARTVVIVRE"
     gene            3332..3781
                     /locus_tag="VGT44_18225"
     CDS             3332..3781
                     /locus_tag="VGT44_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEV2582801.1"
                     /translation="MTTNASAPAKLFYDDVQVGDNIPALEKAPVTHYQLVRYAGASGD
                     FNPLHTDPKVGEMIGVGGIISHGMLIMGFVGQLLSDYVGPAALRKFGVRFKGMTHLDD
                     VITCTGTITEKYEENGEFLIAGKVQAADQNGDVKVTGSFVAALPRRS"
     gene            3835..4662
                     /locus_tag="VGT44_18230"
     CDS             3835..4662
                     /locus_tag="VGT44_18230"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2582802.1"
                     /translation="MGMLDGKVAIITGSGRGIGAATARLLGSEGARVIVSDLDPTPAE
                     ETAAAIRAAGGRAAVIAGDVTDPAFPKQLIQGTLDAFGGIDIIVNNAGYTWDAVIQNM
                     TDKQWYAILDVHTTAPFRILREASHFIRDAAKKEAAANGQANPRKIINISSVSGVYGN
                     AGQANYSTAKAGITGLTKTIAKEWGRFNVQSNCVCYGFIDTRLTASKEGAEKIQREGE
                     EIALGIPDQMRQMAALTISLGRPGTPQEAAGPVLFLASPLSNYVSGIVLEVTGGRAL"
     gene            4845..7514
                     /locus_tag="VGT44_18235"
     CDS             4845..7514
                     /locus_tag="VGT44_18235"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2582803.1"
                     /translation="MLDKLAQQSLKALLHKNQVFTDMASRLSYEADGGIDRAVPDGVV
                     LPQSAQDVTRVMDWAAQQGVPLVGRGAGTGLSGGAVAEHGGLILEFSRMNHLLEIDVW
                     GRTALVEPGLINLRLDEQARERDLYFPPDPASQRASTIGGNVSENSGGPHCFKYGVTT
                     NYIMGMQVVLADGRIAQVGGPAFDYPAYDLCGLLTGSEGTLALMTAITARLVRNPPAA
                     QTLLAVFDSVEQAGKAVSAVIAAGLVPATMEMMDQKITRIVEDYVHAGLPIDAEAILI
                     IDMDGYPASLDAQSAEIARILQAHGGYNLRTAQSAEERAQIWFARKSAAGALAKLSPA
                     QYTIDITVPRSRLAEALVEVNEICDRHQLRVGYVFHAGDGNLHPLILIPDPQDKALMA
                     RVLAAGRETVEVAVRKNGSLSGEHGIGIEKRAFMPLMFNEAEMSAMLDIKHVFDPQNL
                     LNPGKIFPESPSTQTTELSPRLPNDPSVQTVTNAIAPASVAETSRLLQDCTQARQPVH
                     ITSKTGDIHTSNHVTLSTSVLRGIISYAPEDMYITVGAGTPLSEVQEFLAVHGQQVPL
                     ASPWPDATIGGLVAANTNAPLRMRYGAIRDLVLCATVALADGRIIRAGRPVIKNVAGY
                     DLVKLFVGSRGTLGLLTDITLKVFGLTRARRSLVVPFDDLRYGLIQARQLYPRSLVAS
                     AILLVSSEHILETIQHVPGNIPTPHIRDTFKGCPYTLIYTVEGLAEDVQAEIEQVRAA
                     LQADEAAGVALGEPVEIEGISGTDVWTHFLGQASKAVQLRIGLPVRDLPVYVQDQATL
                     LAGGDFLVDYASGFVYCLLPAQDVASVAKTVEQLRASTLQAGGYAIVTHLPDEWTGII
                     DRWGYLPDTLDMMRKLKARWDPAGILNSGEFVV"
     gene            complement(7530..8132)
                     /locus_tag="VGT44_18240"
     CDS             complement(7530..8132)
                     /locus_tag="VGT44_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEV2582804.1"
                     /translation="MTTTSDRLPMVMRADWVSGPQQGRWTYKEYAALPNDGNRYEVVN
                     GVLYMSPAPSGSHQDAALEIASYLRAHVKLTGLGLVRIAPFDVELAPNVVVQPDVLVV
                     LKENFDKVADNRIIGAPDLVVEVASPSTAIYDRHDKLIAYARAGVPEYWIVDPDARTV
                     EVLILEAPGMYLSQGIFRGKSLLPSQAVPNFSVHVEQFFD"
     gene            complement(8191..9006)
                     /locus_tag="VGT44_18245"
     CDS             complement(8191..9006)
                     /locus_tag="VGT44_18245"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV2582805.1"
                     /translation="MLEYEILARGLYRPDQVRVTYDPALRMPITPAIQSWMDDYWERR
                     LAIAKTQGYPLFDTPLYRYISVETCDDSTLQLLVGDTGYKEYVTTRAPEFFNGRGRQE
                     LGNALSVCSVVETSDGLILLDKRQGVDVYAGRYHVIGGYFERDRDNALDGQPDPFAAM
                     RREIREETGITGEDIQEQFCLGLVYDVATPHAELCFLTRLGITLAETQTRVPEEDEIK
                     QLETLQVTTESLRAFLLTHHGHISATGEPNLLLYGALLFGHNWMDDIVYRWSK"
     gene            complement(9010..9846)
                     /locus_tag="VGT44_18250"
     CDS             complement(9010..9846)
                     /locus_tag="VGT44_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582806.1"
                     /translation="MANSSRRPSRKQPDDEKRAESAPLKFPVDPDRHQGPWEDDDINV
                     DDDEIASDPPRSPTSAIRYQPSPPAGSQRRTGTRTDRDTTRQPTPNVPGGQPRRAGQV
                     SDAFPGPVAVRAGRTTATGNAPGAAAPPPVYRRSWQSLHWMFYVGVGMIAALALWFSF
                     STILAWGTAKYNDLVYGYPRFYQTDAVVGHGDSAAHPSHFVAMNLHGQVIVIELPASN
                     PSKSYEYIGPDLIASGDDLIPITLSFSDVNHNGKPEMIIHIENREVIFCNDGAKFTQC
                     SS"
     gene            complement(10048..10935)
                     /locus_tag="VGT44_18255"
     CDS             complement(10048..10935)
                     /locus_tag="VGT44_18255"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2582807.1"
                     /translation="METARAEVLHVLRRDPRQCGQDTSRWTLASLLGQLPQVQLRTPA
                     GLWLHLQRLGIHYKRARDYVHSPDTHYQAKLAVVDTLQTQVQASAGAEVLLYQDELTF
                     YRQPSLAWAYEQAGKTQPLARRSYRSNTPTRVVSTLDAHTGGVLFRRGSKIGIHELVA
                     FYQQVVAAYPHAQRFWIVLDNWPIHFHPDVLVALEAQETVFPFPRPPSWPQQPSAAAQ
                     KAWGALHLPIQFVPLPTYASWCNPIEKLWRWLQQDLLHLHRLADDLSALREAVDAFLR
                     DFAGSSQALLRYVGLHVPY"
     gene            complement(10943..11218)
                     /locus_tag="VGT44_18260"
     CDS             complement(10943..11218)
                     /locus_tag="VGT44_18260"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2582808.1"
                     /translation="MPHLRTLQLSEAAHESLEEMSKHHPKAYLRERAAALLKIERGRR
                     PAEVARTGLLRCRKPDTVYTWLDRYEQFGLRGLYLRAGRGRKLGRSP"
     gene            11928..13628
                     /gene="nadB"
                     /locus_tag="VGT44_18265"
     CDS             11928..13628
                     /gene="nadB"
                     /locus_tag="VGT44_18265"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEV2582809.1"
                     /translation="MANSQPFDVVIIGGGIAGLSVALRLPASLRVALFTKGQLGESNT
                     RYAQGGLTAALGADDSPELHTQDTLVAGAGLCDENAVRILAQSAPAAVQWLIESGVRF
                     DRAQPGDEHATPGGLLLGREAAHSRWRVLHAGGDATGAEIERALTTALRKRPSITVYE
                     ETHVRRLLVEDGVCVGLEAAHRVGQTFVVNAEATVLASGGAGGLWQHTSNPAGATADG
                     LALAWRAGAALTDLEFMQFHPTVLAANGGSHLISEAVRGEGAYLRNHAGERFMPRYSD
                     LAELAPRDVVARSILSEMLREGVDCQYLDLRHLPAERMRQRFPTISAICRGYGLDLAQ
                     DLLPVAPAAHYCMGGVMTGINGHTSIPGLYAVGEVACTGVHGANRLASNSLLEGLVFG
                     LRLADSLSRGTDAGVETMQPSRRAITVPLAREIDRYAVLLQCQPGEQTMLQARSELRR
                     IMWQYVSLSRDQAGLLEACARLDSLQTDLLAHQPSRPAAEETAPAWQETVNMFEVADL
                     VIAAALQRRESRGSHWRTDYDYLDDALTGAHYVLTRPQKADQSRIYKKGIPSYATIDA
                     "
     gene            13609..>14307
                     /gene="nadC"
                     /locus_tag="VGT44_18270"
     CDS             13609..>14307
                     /gene="nadC"
                     /locus_tag="VGT44_18270"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV2582810.1"
                     /translation="MQQSTLDLIRRALDEDGVDADVTTLATVPARQQVRASIIARQAG
                     VIAGLAVAAAVFREVDPAIAVNLEVADGAPVQPGQVIAILSGPARPLLSAERTALNFL
                     GRLSGIATLTARCALAVEGTRARILDTRKTTPGLRSLEKEAVRLGGGHNHRTGLYDGV
                     LIKDNHIKAAGGITPAIDAARRSAHHLLKIEVECETLDEVREALTAHADVILLDNMDI
                     ESMRRAVEVIRGAAP"
BASE COUNT         2976 a         4352 c         4153 g         2826 t
ORIGIN      
        1 gagataccga caatttgttt catataatag taactccttt ccctttatcc ccaggacata
       61 gcgaacaacc agggcgaccg cctcgcacga ctggtcgtca aatgtcattg gtcgtcgaat
      121 gtcatcctga gcgcagcgaa ggatctcagc ccccggagcg tcagatcctt cgctgcgctc
      181 aggatgacag gctcggttgt ccccggtaca tcacgccgtc ttgatttgcg cggcctcttc
      241 caaccccagc gacttgacgg cgttctggcg cagcacgaac ttctgcacct tgccactggc
      301 ggtcagggga taggattcga tgaactggaa gtagcgcggg attttctggt ggctgatctg
      361 gcctttgcaa tactcgcgca gttccgcctc ggtcaacgtc tcgccttgct gtggaatcac
      421 aacggccagc agctcttcgc cgaagaattt atcggggacg ccgacaatct gcacatccgc
      481 aatcctggga tggcgaatta gaaattcttc aacttcgcgc gggaagatgt tttcgccgcc
      541 gcgaatgacc atatctttca ggcgacccac aatgttgatg tagccctgtg cgttcatggt
      601 cgccaggtcg ccggaatgca gccagccctc gctgtcgatc gcctcggcgg tcttctccgg
      661 catatggtag tagccggaca tgaccaggta gccccggcag accagttcgc cgcgttcgcc
      721 gcacggcaca gcttcacccg tggcgggatc gacaatcctg accgctccat gtggcatcgg
      781 cacgcccacc gtcgaggatt tcaactcaaa cgagtcctcg cgtttcgtca gcgtaatggc
      841 cgcgctggcc tcggtttgcc caaaaacgat ggttgcgtcc gcgcccatgc gctccttgac
      901 ctgctccatc aggtagactg gaacgggcga gccgccgcta acaacgcttt tcaatgagga
      961 gaggtcgaaa ctgccaaaat ccgggtgctg caagatggcc agcagcatcg taggaacgcc
     1021 accaaagtag ctgcatcgct cgctgctgag aatctgcaag gccttgagcg gatcgaaagc
     1081 aatcagcgga tgcaacgtgc cgccgatgcc cagcgtggcc ataatgccca gcacacaacc
     1141 acctgcatgg aagaacggca tagcagtgca aacatgctcc cctggttgta tgccccagcg
     1201 cagggcaaag atgcggccat tgttgaggat ggcgcgctgc gtctggatgg cgcctttggg
     1261 gaaaccggtc gttcccgatg tatagagaat ctgcgccggg tcgccaggct gcaccgctgc
     1321 ctgccgttcg cgcagcgcct ccatgctgac ccgctcaccc gccgcaacca tctcgcggaa
     1381 aagcgcgggt cgccagcctt cctgctctaa caggccagcc gggggcgcgc ccagcaggct
     1441 gacatagcgc agatgaggca gcatctcgct gctgacttcg ccatgcgcag cgcccggtgt
     1501 gacgcgctcg cgcacggtcg ccaggcaatc atgatcgcgc acacgcgcca tgaagaacag
     1561 ggccgccaca tcgccctgtt tcaaaatata ttcaacctcc tgcgcgcgca aaaccgggtt
     1621 caccgtgacg agaaccagcc ccgcctttga cgctgccagt tcgagcagag gccattccgg
     1681 caggttgatg gcccagacgg ctatatgctc ccctttttgc aggcccagtg ccatcaggcc
     1741 gcgcgccaca gcatcaaccc gctcgcgata ctcgcgatac gtccagcgaa tatcgagcgc
     1801 gccgccaaat tcgggatagc atgagtagac cagcgcctcc cggtcgggca tctcctccgc
     1861 gcgcacgtcc aacaactggc caggagtcat ctccaggatt tcaataccgc catcttcggc
     1921 ctgccagttc gacagaccat cattggtcat gcccatcagc attctccttg ttgctctcca
     1981 caaaacgtgc ttgtagcata ccttctatga agctgagttg caagcaatca gcccctataa
     2041 tgtgtgaatt ttatttgggc atcaatcaac cgtaggagcc ggttcaccgc gctcaccacc
     2101 gatttatcgg cctgtccatg taccttaccg acacgcatcc caggccaatg atgaacgttc
     2161 ccaaccaaag tcggaaatgt catcctgagc gaagcgaagg atctgtcagc ccatccactg
     2221 agacccttcg cttcgctcag ggtgacatac tttacccgaa actgggcaaa ttcctcgcga
     2281 acgggctgcc ggtatccggc gggcggtcaa cgacggcaac cgtgcagcgc gaaatgcaga
     2341 ccagcttatc gttctcgtcg gttatgcgaa catcccagac gtgcgtcgtg cgcccgcgat
     2401 gtacaggcgt agcagtggcc gtcaactgcc catcgcgttt gggccgcagg tgattggcgt
     2461 tgatctccag gccaaacgcc atctgatttg ccgcgtcgat attcagcacc gtgccgagcg
     2521 aggcgacgga ctccgccagc gtcaccgaga taccgccgtg caaatagccc acctgctggc
     2581 gatggttcgg gccaatcggc atggtcgcaa ccaccttgtc cttctccgcc gtcaccaagc
     2641 gaatatcggg ccattcccac accgatcccg tgcgggtgct atttatcact tctacgatgt
     2701 tttcgctgga aatatccatg tgcaatacct cttacagttg aatgtactca tcgagggcga
     2761 ccatctccct tattccatat tatcgcacac cgtccttgac aagccgcttt gcgaaaaggg
     2821 atactatctg tacaatctgg ttgtacagca aaggagcaac ctttcatgtt cgacaaaagt
     2881 aatattggcc gcagctttac ccccttcact atcgaggttg agcgcagcaa gattcgtgaa
     2941 atcgcgcagg ccatcggcga tgataaccct atttatcaaa gcacggcagc ggcccaggcg
     3001 gcagggtaca aagatatccc gctctacccg acttctccaa cctccttcgg cttctgggga
     3061 aatcgcggca tgggaatgca gctggcaagc accgggctga acgtcatgcg catcctgcat
     3121 ggcgaagagg agtacgagta tctcgcgccc atctaccccg gcgatacgct caccggcgtc
     3181 gtcaccattc tggacggcaa gacgcgccag gggcaggccg gctcaatgga tatcgtcacc
     3241 accgagacaa gctataccaa ccagcacggc aagcccgtct tgaaagcccg caccgtcgtg
     3301 atcgtgcgcg aataacaagg agaaccccct aatgaccacc aacgcttcgg ctccggccaa
     3361 attgttttac gacgatgttc aggtaggcga caacatcccg gccctggaaa aagcgccggt
     3421 cacgcattat caactggtgc gctatgccgg cgcgtcgggc gatttcaacc cgctgcacac
     3481 cgacccgaag gtcggcgaga tgatcggcgt gggcggcatc atctcgcacg gtatgctgat
     3541 tatgggcttc gtagggcagc tgttgagcga ttatgtgggg ccagccgcgc tgcgcaagtt
     3601 cggcgtacgc ttcaagggca tgacccacct cgacgatgtg attacctgca ccggcacgat
     3661 taccgaaaaa tacgaagaga acggtgaatt cctgatcgcc ggcaaggtgc aggctgccga
     3721 ccagaacggc gatgtcaagg tgacaggcag cttcgtggca gccctaccgc gccgctcgtg
     3781 acctatcgct gagcatgaca atcgtcacat ttcattatga ggagaaatac acatatgggt
     3841 atgcttgatg gcaaagtagc cattattacc ggctccggac gcggcatcgg cgcggccacc
     3901 gcgcgattgc tcggctcaga gggcgcgcgg gtgatcgtga gcgacctcga tcctacgccc
     3961 gccgaagaga ctgccgccgc cattcgcgcc gccggcggac gggccgccgt aatcgcgggc
     4021 gatgtgactg acccagcctt ccctaaacag ctcattcaag gaacactgga cgccttcggt
     4081 ggcattgaca tcatcgtcaa taacgctggc tacacctggg atgccgtcat ccagaacatg
     4141 accgacaagc agtggtacgc catcctggac gtgcatacaa ccgcgccctt ccgcatttta
     4201 cgcgaagcct cgcatttcat tcgcgacgcg gcgaaaaagg aggctgccgc caatggccag
     4261 gccaatccgc gcaaaatcat caacatcagc tccgtctcag gcgtttatgg caacgcaggc
     4321 caggccaact actcgacggc caaagcgggc atcaccggcc tgacgaagac gatagcgaag
     4381 gaatggggcc gtttcaacgt gcagtccaac tgtgtctgct acggcttcat cgacacccgc
     4441 ctgaccgcct ctaaagaggg cgcggaaaag atccagcgcg aaggcgaaga aatcgccctg
     4501 ggcattcccg accagatgcg ccagatggcc gcgttgacca tctcactcgg tcgcccaggc
     4561 acaccgcagg aagccgccgg cccggtcctg ttcctcgcct cgccgctctc gaattacgtc
     4621 tctggcatcg tcctggaggt cacaggcgga cgggccttgt aaacccggtt tattggttcc
     4681 gtaggggcgg cggcggtgga agatgtgggg aggggacgct tgcgtcgccc gcgtacctgt
     4741 tcatgtgaag gaatcgtcaa tccaacgggt tacggcgggc gacgcaagcg tctcctcccc
     4801 acatcttccg ccgccgccgc ccctacggat gtagaggaca tgttatgctc gacaaactcg
     4861 ctcagcaatc gctaaaagcc ctactacata aaaaccaggt cttcaccgac atggcttcgc
     4921 ggctctccta cgaggcggat gggggcatcg atcgcgccgt gccggatggg gttgtgctgc
     4981 cgcaatcggc gcaggacgtg acgcgggtca tggactgggc ggcgcagcag ggtgtgccgc
     5041 tggtcgggcg cggcgcggga acgggcctat cgggcggcgc ggtggccgaa catggcgggc
     5101 tgatcctcga attttcccgc atgaaccacc tattggaaat cgatgtgtgg gggcgcacgg
     5161 cgctggtgga acccggcctg ataaacctgc gcctggacga gcaggccagg gagcgcgacc
     5221 tctattttcc gcccgatccg gccagccagc gcgcctcgac catcggcggc aacgtctccg
     5281 agaattcggg cgggccgcac tgcttcaagt acggcgtcac caccaactat attatgggga
     5341 tgcaggtcgt gctggccgat gggcgtatcg cgcaggtcgg cggcccggct ttcgattatc
     5401 ccgcctacga tctctgcggc ctgctgaccg gcagcgaagg tacgctggcg ctgatgaccg
     5461 ccattaccgc ccggctggtg cgcaacccac cggcagcgca aacgctgctg gccgtcttcg
     5521 actcggtcga gcaggcaggc aaagcggtct cggcagtcat cgcggcaggg cttgtcccgg
     5581 ccacgatgga gatgatggac cagaagatca cacgcattgt agaagactac gtccatgccg
     5641 ggctgcccat cgatgccgaa gctattctga ttatcgacat ggatggctac cccgccagcc
     5701 tggacgcgca gagcgcggag atcgcgcgta tcctgcaagc tcatggcggc tacaacctgc
     5761 gcacagcgca gagcgcggag gagcgcgccc agatctggtt cgcgcgcaaa agcgcggcgg
     5821 gtgcgctggc caaactttcc ccagcgcagt acaccatcga catcaccgtg ccgcgtagcc
     5881 gcctcgcgga ggccctggtc gaggtcaacg aaatctgcga ccgccatcaa ttgcgcgtcg
     5941 gctacgtctt tcatgccggt gacggcaacc tgcacccgct cattctcatc cccgatccgc
     6001 aggacaaagc tttaatggcg cgcgtcctcg ccgccgggcg cgaaactgta gaagtggccg
     6061 tgcgcaaaaa cggcagcctg agcggagaac atggcatcgg catcgaaaaa cgcgccttta
     6121 tgcccctcat gttcaacgaa gccgagatgt cggccatgct cgacatcaaa cacgtcttcg
     6181 atccacaaaa cctgctgaac cccggcaaaa tattcccaga atcaccgtca acgcaaacaa
     6241 ccgaactttc gccccggttg cccaacgatc catcagtcca aacagtcaca aatgcaatcg
     6301 cccccgcatc cgtagcagag acatcccgcc tcttgcagga ttgcacacag gcaaggcaac
     6361 ccgtccacat caccagcaaa acaggcgata tacatactag caaccatgtc accctcagca
     6421 catccgttct gcgtggtatc atttcctacg cgccggagga tatgtacatt accgttggcg
     6481 cgggaacgcc gctgagcgaa gtgcaagaat tcctggcagt gcatgggcaa caggttcctc
     6541 tggcgagtcc ctggcccgat gctacaatcg gcgggctggt agcagccaat acaaacgcgc
     6601 cgctgaggat gcgctacggc gctattcgcg acctcgtgct atgcgccacc gttgcgctgg
     6661 ctgatgggcg aattattcgc gccgggcgac ccgtcatcaa aaacgtggcg ggctacgacc
     6721 tggtaaagct tttcgtcggc tcgcgcggca ccctcggcct gctgaccgac atcacgctga
     6781 aagtgttcgg attgacgcgc gcgcgacgct cgctcgtcgt ccccttcgat gacctgcgct
     6841 acggcctgat ccaggccagg cagctctatc cccgctcgct cgtggcatcg gcgatcttgc
     6901 tggtcagcag cgaacatatc cttgagacga tacagcatgt gccgggcaac atccctacgc
     6961 cacatatacg ggacaccttc aaggggtgtc cctacacatt gatctacacg gttgaagggc
     7021 tggccgagga tgtgcaggcc gaaattgagc aggttcgtgc ggcattacaa gcagatgagg
     7081 ccgcaggagt agcgttggga gagccggtag aaatagaggg catttctggc accgatgtct
     7141 ggacgcattt cctggggcaa gcgtctaaag ctgtacagtt gcgcattggt ctgcctgtgc
     7201 gcgacttgcc tgtatatgtc caggaccagg cgacgctgct cgctggtggc gacttcctgg
     7261 tagattacgc cagtgggttc gtctactgcc tcctgcctgc tcaagacgtg gcatctgtcg
     7321 cgaaaacggt tgagcagttg cgcgcgtcca ctctgcaagc cggtggttat gccattgtga
     7381 cacatctgcc ggacgaatgg acaggcataa ttgatcgatg gggctacctg cccgatacgc
     7441 tggatatgat gcgcaagctc aaggcacgat gggacccggc ggggatattg aacagtggag
     7501 aatttgtggt ttaacccttt gctcaaagac taatcgaaaa attgttcgac atgcactgaa
     7561 aaattcggca cagcctgcga tggaagcagg gacttcccac gaaagatacc ttgcgacaaa
     7621 tacatccccg gcgcttctag aattagcact tcaaccgtgc gagcgtcggg atcaacgatc
     7681 cagtattccg gcacacctgc gcgcgcataa gcgatcaact tatcatgtcg atcatagata
     7741 gctgtgctgg gagaggctac ttcgacaacc aggtcaggcg ctccgatgat gcggttgtca
     7801 gcgactttat caaaattctc cttcaaaacc acaagcacat ctggttgaac aactacattg
     7861 ggcgccagtt caacgtcaaa tggggcaatt cgtaccaatc caagcccggt cagtttgacg
     7921 tgagcgcgta aataagaagc aatttcaagt gcggcatctt gatgcgatcc gctaggcgct
     7981 ggcgacatat aaagcactcc attcacaact tcgtatcgat tgccatcgtt cgggagagct
     8041 gcatattctt tataggtcca gcggccttgt tgagggcctg aaacccaatc agcgcgcatt
     8101 accataggga gtctgtcaga tgttgtcgtc atgtcagccg cgcctttcgt agaagcaaac
     8161 gataccactt tattatacgc tatcagacga ttacttgctc cacctgtaca cgatatcatc
     8221 catccaatta tgtccaaaga gcagcgcgcc gtagagcagc agattcggct cgcccgtggc
     8281 ggaaatatgg ccgtgatggg tgagcagaaa ggcacgcagg ctctccgtcg tcacctgcaa
     8341 ggtttccagc tgcttgattt catcctcttc cggtacgcgc gtttgcgtct cggccagcgt
     8401 gataccgagg cgcgtcagga agcatagttc ggcatgagga gttgcaacgt cgtagaccag
     8461 gcccagacag aattgctcct ggatgtcttc gccggtgatg cccgtctcct cgcgtatctc
     8521 gcggcgcatg gcggcgaacg ggtcaggctg cccatcaagt gcattgtcgc ggtcgcgctc
     8581 gaagtagccg ccgatgacgt ggtagcggcc cgcgtaaaca tccacgccct gccgcttatc
     8641 aagcaggatc aggccgtcgc tcgtttccac aacggagcag accgagagcg cgttgccaag
     8701 ctcctgacgt ccgcgcccat tgaaaaactc aggggcgcgc gtggtgacgt actccttata
     8761 gccggtatct ccaaccagga gttgcagcgt gctatcatcg catgtttcca cgctgatgta
     8821 gcgatagagg ggcgtgtcga atagcggata gccctgcgtc tttgcgatag ccagcctgcg
     8881 ctcccagtag tcgtccatcc agctttgaat cgccggagtg atgggcatac gcagagccgg
     8941 gtcgtaggtg acgcgcacct gatcggggcg gtagagacca cgcgcgagaa tctcatattc
     9001 gagcatgggt cacgagctac attgcgtaaa cttggccccg tcgttgcaga aaatgacctc
     9061 gcgattttca atatggataa tcatttcggg cttaccattg tggttgacat cgctgaacga
     9121 aagggtaatc ggtatcaggt catcgccgct ggcaatcaga tcagggccga tatattcata
     9181 ggacttcgag ggattgctcg ccggtaattc gatcacgatc acctgcccat gcaggttcat
     9241 agcaacgaaa tggctggggt gcgcggcgct atcgccatga ccgacgaccg cgtcggtctg
     9301 gtaaaagcgc ggatagccat agacgagatc gttatatttc gcggttcccc acgccaggat
     9361 cgtcgaaaac gaaaaccaca gcgccaacgc ggcaatcatg cccacaccca cgtagaacat
     9421 ccaatgcagg gactgccagg agcgccggta caccggcggt ggcgcggcgg cacccggcgc
     9481 gttgccggta gcagttgtgc gcccggcgcg caccgccacc ggtcctggga aagcatcgga
     9541 cacctggcct gcgcggcgcg gctgcccacc agggacgttc ggggttggct ggcgagtcgt
     9601 atctcgatcc gtgcgcgttc cagtgcgacg ctgactgcct gctggtggcg aaggttgata
     9661 gcgaatggcg ctggtcggcg aacgaggcgg atcagaagcg atctcatcat cgtctacgtt
     9721 tatatcgtca tcttcccagg gcccctgatg cctatcgggg tcaaccggaa acttcaacgg
     9781 tgccgactca gcccgctttt cgtcgtcggg ctgcttccgt gaaggtcgcc tgctgctatt
     9841 cgccattgcg catcctttct gatagcgcga actgtcgcgt acagcaatgg tactacgctc
     9901 atccacaaat tgcaatcaat cacgaatgac tgagcctatt gtacgtacca cgcttctagc
     9961 aattgtatca caatgcatca atttgtatca tagtggcctg tcaaggttta aacgaaatgt
    10021 aggggcgcaa tgatgaactt taacaaatta atagggtaca tgaaggccga catagcggag
    10081 taaggcttgc gaggagccag cgaagtctcg caagaaggca tccaccgcct cacgtaaggc
    10141 cgacaagtca tcggcgagcc gatgcaagtg gagcaggtct tgctgaagcc agcgccagag
    10201 cttctcaatc ggattacacc aggaggcata ggtcggcaaa ggcacgaact ggatgggcag
    10261 gtgcagcgcg ccccaggctt tctgggcggc cgcgctgggc tgctggggcc aactgggtgg
    10321 gcgagggaag ggaaagaccg tctcttgcgc ttccagggcc accagcacat cgggatggaa
    10381 atggatcggc caattatcca gcacgatcca gaagcgctgg gcgtggggat aggccgccac
    10441 gacctgctga tagaaggcca ccagttcgtg gatgccgatc ttgctgccgc gtcgaaagag
    10501 gacccctccg gtgtgggcat ccagcgtact gaccacgcgc gttggcgtat tgctgcgata
    10561 ggagcgccgg gccagtggtt gggtcttgcc ggcttgctca taggcccagg ccaggctggg
    10621 ctggcgatag aaggtcagtt catcctggta gagcagcacc tcggctccgg cgctggcttg
    10681 cacctgcgtc tggagcgtgt ccaccaccgc gagcttggcc tgatagtgcg tatcggggct
    10741 atgcacatag tcacgggcgc gcttgtagtg gatgcccagc cgctgcaagt gcagccacag
    10801 cccggccggc gtccgtagct gcacctgggg caactgcccc aagaggctcg ccagcgtcca
    10861 gcgcgaggtg tcctgcccgc attggcgcgg atcgcgccgc agcacgtgca gcacctccgc
    10921 gcgcgcggtc tccaccgact gctcagggag accgtcccag cttgcgtccg cgccccgcgc
    10981 gcaggtagag accccgcaga ccgaactgct cgtagcgatc aagccacgta tagaccgtgt
    11041 cgggcttgcg gcaccgcagc agtcccgtgc gcgccacctc tgcgggccga cgcccgcgct
    11101 caatcttcag taaggcggca gcgcgttcgc gcagataggc tttcggatga tgcttgctca
    11161 tctcttccaa gctctcatga gcggcttcac tcaactggag ggttcgtaaa tgcggcatcg
    11221 gggggactcc tttctgggtc tcgaggcctt catccattcc ctcctagtat accctattaa
    11281 tttgttaatg ttcataattg cgcccctaca cgcctaatcc atcttgataa aacattacga
    11341 tacgatgcat tgaaaaacag caataaaaaa cgcccctcat cccatgccag ggacgagaag
    11401 cgcgatgctt tcgtggtgcc acccatgttc agatcaatcg cgactgtcgt gcaaacaatc
    11461 gaatcgatcc ctttgtgccg gatacgctct gatatcctgt cctcgataac gggggacgtt
    11521 ccgaatgagt cctacttgct ccggctggaa cgttcgctca tttgctccca ggcccattca
    11581 acgaaggcgt tggtgccggg ttccaccgct cccgactctc taggacacac gcccgttcgt
    11641 ctactcttcc tgttccgcgc gttacgtatg cggttgtttc tggtgtaagt ataccgacga
    11701 cgttgtggtt tgtcaagcat tttttcgctc atttcaccac cagccggtct aatcagtgga
    11761 tgaaatcgct atatcgttag acttgctttt ccctcgattt ggtggtaaac tttttttcac
    11821 gggagttagt gtacaaagaa caccttttta atttgtcttt ggacaaaacg ttttttttac
    11881 aattatgtaa gtgtacaaag tacacttact cgaccaggag caacgcaatg gcaaacagtc
    11941 agccgttcga tgttgtcatc atcggcgggg gtatcgcggg attatcggtg gcgctgcgcc
    12001 tgcccgcctc cctgcgcgtg gcgctattta cgaagggaca acttggagaa agtaatacgc
    12061 gctacgctca gggagggttg acggcggccc tgggcgcgga cgatagtccc gagctacaca
    12121 cgcaggatac gcttgtcgcc ggagctggtt tgtgcgatga gaacgccgtt cgcatcctgg
    12181 cacagtcggc tccggcggct gtgcaatggc tgatcgagag cggtgtgcgc ttcgaccggg
    12241 cgcagcctgg ggatgaacac gccacgcccg gcgggctgct gctgggacgc gaagcggcgc
    12301 acagtcgctg gcgtgtgcta cacgccggcg gcgacgcgac gggagcggag atcgaacgcg
    12361 ccctgacgac tgctctgcgg aagcgtcctt ccatcaccgt ctatgaagag acacatgtgc
    12421 gccgcctgct ggtggaggat ggcgtctgtg tcgggctgga agccgcgcat cgcgtaggac
    12481 agacctttgt tgtgaacgct gaggcgacgg tgctggcgag cggtggcgcg ggcggtctct
    12541 ggcagcacac ctcgaacccg gcaggcgcga ccgccgatgg cctggccctg gcgtggcgcg
    12601 cgggcgcggc gctgaccgac ctcgaattta tgcaatttca tccgaccgtg ctggcagcaa
    12661 acggcggttc gcacctgatc tcggaggctg tgcgcggcga aggcgcgtac ctgcgtaacc
    12721 atgccggtga acgcttcatg ccgcgctact ctgaccttgc ggaactggcc ccgcgtgatg
    12781 tcgtggcgcg ctccattctc agcgagatgc tgcgcgaagg cgtcgattgc cagtacctcg
    12841 acttgcgcca tctcccggca gagcgtatgc gccagcgctt cccgaccatt tccgccatct
    12901 gtcgcggcta tggtctggac cttgctcagg atttgctgcc ggttgcgccc gccgcgcact
    12961 attgcatggg cggcgtcatg accggcatca atggacatac cagcatccct ggcctgtacg
    13021 ccgttggcga ggtagcctgt accggtgtgc atggcgcaaa ccgactcgcc agcaactcgc
    13081 tgctcgaagg gctggtcttc ggcctgcgcc tcgctgatag cttgtcgcga gggacagatg
    13141 cgggcgtcga aaccatgcag ccatcccgcc gggccataac ggtcccactt gcgcgcgaaa
    13201 tagatcgcta cgccgtgttg ctacaatgcc aacctggcga acaaaccatg ctccaggcgc
    13261 gcagcgaatt gcggcgcatc atgtggcaat acgtctcgct cagccgcgat caagctggct
    13321 tgctggaagc gtgcgcccga ctcgacagcc tgcaaaccga tctgctcgcc catcaaccat
    13381 cgcgtcccgc cgctgaggag actgctccag cctggcagga aaccgtgaac atgttcgagg
    13441 tagcagacct ggtaatcgcc gccgccttgc agcgacgcga aagccggggc agccactggc
    13501 gcacggatta cgattatctg gatgatgccc tgaccggcgc gcactacgtg ctgacgagac
    13561 cgcagaaagc cgaccaatcc aggatatata aaaaggggat accatcttat gcaacaatcg
    13621 acgcttgacc tgatccggcg cgcgctcgat gaagatggcg tggatgctga tgtgaccacg
    13681 ctggcgaccg ttccggcgcg gcagcaggtg cgcgccagca ttatcgcgcg ccaggcaggg
    13741 gtcatcgccg gtctggcggt agcggctgcg gtgtttcgtg aggttgatcc ggcgattgca
    13801 gttaatctgg aagtggccga tggcgcgccg gtacagcccg gccaggtcat cgcgatcctg
    13861 agtggcccgg ctcgcccgct gctcagcgcg gaacgtaccg ctttgaactt cctgggtcgc
    13921 ctgtccggga ttgccacgct gacggcgcgt tgcgcgctgg cggtcgaggg aacgcgggcg
    13981 cgcatcctcg atacgcgcaa aacgacgccc ggactgcgga gcctggaaaa ggaagcggtg
    14041 cgcctcggcg gaggacataa ccaccgcacc ggcctgtacg atggagtgct gataaaggac
    14101 aaccacatca aagctgccgg tggcatcacc ccggccatag acgcggctcg tcgctcggca
    14161 catcacctgc tcaagatcga ggtcgagtgc gaaacgctgg acgaggtgcg cgaggcgttg
    14221 actgcgcacg ccgacgtgat cctgctggat aacatggata tcgagtcgat gcgccgcgcg
    14281 gtcgaggtca ttcgcggcgc agctccc
//
LOCUS       DASZAK010001036         5619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_632519, whole genome shotgun sequence.
ACCESSION   DASZAK010001036 DASZAK010000000
VERSION     DASZAK010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632519"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..659)
                     /locus_tag="VGT44_18275"
     CDS             complement(180..659)
                     /locus_tag="VGT44_18275"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEV2582811.1"
                     /translation="MRNAVREHRTAYDFEMRRRHIPRVLLSDGTESSAIVADAEGSSQ
                     GVLRGTPASPGTVTAKARVILDPTGAHLAPGDILVAPSTDPGWTPLFLTAGGLVMEMG
                     GAISHGAVVAREYGIPAVVAVAGATERITTGSRITVDGAAGTITIEEEAQAPAEQVR"
     gene            complement(738..2933)
                     /locus_tag="VGT44_18280"
     CDS             complement(738..2933)
                     /locus_tag="VGT44_18280"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEV2582812.1"
                     /translation="MSLPQQGEQYQAQDSSGVFVLPFSLLHRDMLALVGGKAANLGVL
                     TNAKFPVPPGFCVTTNAYALVAADTGLQRLLDTYAAREHGDAKRLEDLAQAARDCMLA
                     ATIPTDIADAIAAAYGSLGSGAPLPVAVRSSATAEDLPFASFAGQQDTYLNIVGVEAV
                     LDAVRRGFASLWTDRAVSYRQSLGLDQSSVKLAVVVQRMVESSVAGVLFTAHPVTGKR
                     RQAVIDANPGLGEAVVSGATNPDHFVVNTATGELVERRLGEKSVVIRGAAGGGTLQSS
                     ESSGRNEACLTDEQIRALADLGARVEAYYGEPQDIEWAIDPEGVLWLTQSRPITTLYP
                     LPTSAPTSEDDLRVYVSANVVQGVYRPFTPAGLSTFRLIASGIAAGFGYPQPDPLSGP
                     PVLIEAGQRLFLDMTPVLRNKVGRRVLIGAASQGEARSAPLLQQLSSDPRLPLIATKR
                     WPLLRAFGRVFEKTRAPIYALQAVFWPKAAVRRVQMTAERVRAMNFITPDLTNEERLL
                     RMEHIVGDFLGVIGGIAPVLPVGLGSQALAGKLLGDLAKPEERQVMLRGLPHNPTTEM
                     DLELWRLAQRVTAHPDIIAHVHECTPEHLAQEYRTGTLPRPLQQGLAEFLAVYGHRGV
                     AEIDLGLPRWSEDQTHILGALANYLQVQAPSLAPSVHFQRGAQEAESMLAELVQRARG
                     KGWMRSRLVHFFLRRARELVGLREMPKFIIVLLMARMRELWWTVGKELK"
     gene            3126..3650
                     /locus_tag="VGT44_18285"
     CDS             3126..3650
                     /locus_tag="VGT44_18285"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2582813.1"
                     /translation="MTPWLADVQAVLSTTPERWQRLVSTLPIELLTRPPATGEWSALQ
                     CLQHLLDAERLVFPVRFHAFLAGEEFVDFDPNEPRAGTTALTPEQLVAAFAGARQGSL
                     LLFQQVTDDDLGRTKQHPKLGTVTLAEMLHTWAAHDLNHTVQAERALIHPFLLGCGPW
                     RTFFRDHEIAGQKE"
     gene            3897..4772
                     /locus_tag="VGT44_18290"
     CDS             3897..4772
                     /locus_tag="VGT44_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEV2582814.1"
                     /translation="MKIYLVDGTYELFRNHFGAPPRKAPDGREVGATLGLLRSLLLLL
                     ITPGITHVACAFDHVIESFRNGLYPGYKTGAGVDPDLIAQFELAEEAVAALGLVVWPM
                     VEFEADDALASATTRFKNKAEVEQIVLCSPDKDLAQLVSGSRIVGWDRRRARLIDEAA
                     VVEKFGVCPPSIPDWLGLVGDAADGFPGIPGWGEKSAAAVLAKYEHLEAIPADPLTWG
                     LTASRAARLAASLAQHRAEALLFRTLATLREDVPLQETLADLEWQGADERLKDICQRL
                     GDDKFPGRVPRWRQS"
     gene            complement(5136..>5619)
                     /locus_tag="VGT44_18295"
     CDS             complement(5136..>5619)
                     /locus_tag="VGT44_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2582815.1"
                     /translation="NTPDILFLGGHCDGWTKSHLLAAVAHDLVQQGHSVLWTPTAQLM
                     QRLLAAKRDLRLPQELAKLHRYACLVLDDIGYVQHDQDEMEVLFTLLSDRYEQRSVCL
                     STNLVFSEWERIFKNPLTTVAAIDRVVHHSVILDMMQVRSFRVQQATDQRAPEANVHS
                     "
BASE COUNT         1157 a         1674 c         1685 g         1103 t
ORIGIN      
        1 aatctgatgc tctggaggca gtgtccacca agcaagctgc gacgggcgat gatgaaccat
       61 cccgctcctg gctttgacac agtgctgacc agttcttcac ttgctcccct ggcaagctac
      121 aaaattgctg agcacggcat cagaactgct tgaaaaagcg caggaggttg tcaaaaggcc
      181 taacgcactt gctcagcagg agcctgggcc tcttcctcta tggtgatcgt cccggctgca
      241 ccatccacgg tgatgcggct tccggtggtg atgcgctcgg tggctcctgc cactgcaacg
      301 accgccggga tgccatactc gcgggccacg acagcgccat gggagatggc cccgcccatt
      361 tccatgacca gcccgccagc cgtgaggaag agaggggtcc agccgggatc ggtggagggg
      421 gcgaccagga tgtcaccagg agccagatgc gcccccgttg gatcgaggat cacgcgcgcc
      481 ttcgctgtca ctgtccctgg cgaggcaggc gtcccccgca gcacgccttg agacgagccc
      541 tcggcatccg caacaatagc gctggattcg gtgccgtcgg agagcagcac gcgcgggatg
      601 tgacggcggc gcatttcgaa gtcgtaggcc gtgcggtgct cgcgcaccgc gtttcgcata
      661 tcttgtcccg ccagtgcagc gtggacctct ggcagcgaga ggaaaaagat gtctccggca
      721 gtttccagcc ggccagccta tttcaattct ttcccaaccg tccaccacag ctcacgcatg
      781 cgcgccatca gcaagacgat gatgaacttt ggcatctcac gcaacccaac cagctcgcgc
      841 gctcttctca ggaagaagtg cacgagccga ctccgcatcc agcccttgcc ccttgcccgc
      901 tggaccagtt cggcgagcat gctttccgcc tcctgtgctc cccgctggaa atgcacagaa
      961 ggagccaagg atggagcttg cacctgcagg tagttggcca gcgcaccgag aatatgggtc
     1021 tggtcttccg accagcgcgg caggccgagg tcgatctcgg ccactccacg gtgtccatag
     1081 acggcgagaa attcggccag tccctgctgg agcggacgag gtaaggtgcc tgtacgatac
     1141 tcctgtgcca ggtgctcggg agtgcattcg tggacatgcg caatgatgtc gggatgcgcg
     1201 gtgacacgtt gtgccaggcg ccagagttcc agatccatct cggtcgttgg attatgcggg
     1261 aggccacgca gcatcacttg ccgctcctcg ggttttgcaa ggtcgcccaa gagcttgcca
     1321 gctagcgcct gactgcccag accgaccggc agcaccggcg caataccgcc gatgacaccc
     1381 aggaagtccc ccacaatatg ctccatccgc aagagacgct cttcgttcgt gaggtcaggt
     1441 gtgatgaaat tcatggcccg tacacgctcg gcggtcattt gcacccgcct gactgcggcc
     1501 ttcggccaga acactgcttg aagggcatag atgggggctc gtgtcttctc aaatactctc
     1561 ccgaaagcgc gcagcagggg ccatcgtttc gtcgcaatga gcgggagacg tggatcgctc
     1621 gagagttgct ggaggagggg tgccgagcgg gcctccccct ggctcgccgc gccgatgagt
     1681 actctgcgtc ccaccttgtt gcgcaacact ggcgtcatgt cgaggaagag acgctgaccg
     1741 gcctcgatca ggaccggtgg gccgctcaaa ggatcgggct gagggtagcc aaatccggcg
     1801 gcaatcccag atgcgatcaa acggaaggtc gaaagccctg ccggagtgaa gggacgatag
     1861 actccctgca ccacgttggc tgaaacatag acacgcagat catcctcgct cgtcggggcg
     1921 ctggtcggca gcgggtagag cgtggtgatc gggcgcgact gggtgagcca gagcacgcct
     1981 tcaggatcga tggcccactc gatatcctgt ggctccccat aatacgcctc gacgcgtgct
     2041 ccaagatcag ccagtgcgcg gatttgctca tcggtgaggc aggcttcatt cctgccggag
     2101 ctttccgagc tttgcagcgt gcctcctccc gcggctcccc tgatgacgac gctcttctct
     2161 cccagcctgc gctcgacaag ctcaccggtc gcggtattca ccacaaagtg atcgggattg
     2221 gtcgcgccgg agacgacggc ttcccccagg ccgggattcg cgtcgatgac agcttgacgt
     2281 cgcttgcctg tcacaggatg agcggtgaag agcacgccgg ctaccgaaga ttcgaccata
     2341 cgctgcacca ccacggccag cttgacgctg ctctgatcga gtcccaggct ctgtcgatag
     2401 ctcaccgccc gatcagtcca cagcgaggcg aagcctcgcc gtactgcatc caacaccgcc
     2461 tcgacgccca cgatgttgag gtaggtgtcc tgctggccgg caaagctggc aaagggaagg
     2521 tcttcagccg tagcagagga gcgcactgcc accgggagag gtgctccaga gcccaaagag
     2581 ccataggctg cggcgatagc atccgcgatg tctgttggga tggttgcggc cagcatgcag
     2641 tcgcgcgcag cctgagccag atcttcgagc cgtttcgcat ctccatgttc tcgggccgca
     2701 taggtgtcca gaaggcgttg caggccagtg tccgcggcaa cgagcgcgta ggcgttagtc
     2761 gtgacgcaga agccgggcgg cacggggaac ttcgcgttgg tcagcacccc gagattggca
     2821 gccttgccac cgaccagcgc cagcatgtca cgatgcagta aactaaaagg cagcacgaaa
     2881 accccagagc tatcttgtgc ctggtactgc tctccttgtt gtggaagact catgtggtgc
     2941 tctccatttt ctgccagctt tggcaggcag aaccatgcct ctcatagtat gagaaagagt
     3001 aagacgaata tggagaaatg tcaatggatg gctaagcatc cgttttttgg ggtatgattg
     3061 aggtacgagg aagtaacata ttgctgttca ctttgggcaa aggatcagac aacaggaggt
     3121 gttcattgac tccctggtta gcagacgtcc aagccgtcct ttccaccact cccgagcgat
     3181 ggcagcggct ggtcagcacg cttcctattg agctgctgac tcgtccaccg gccacaggag
     3241 agtggtcagc gctccagtgc ttacaacatt tactggacgc tgagcgcctg gtttttcctg
     3301 tccgcttcca tgcttttttg gcaggagagg agttcgtcga tttcgatcca aacgaaccgc
     3361 gcgcaggcac aacggcactg actcccgaac agctggtggc ggcgttcgca ggcgcgcggc
     3421 aaggcagcct tctcctgttc caacaggtga cggatgacga tctggggcgg acgaaacagc
     3481 atcccaagct gggtaccgtc acgctggcag agatgttgca cacctgggcc gctcacgatt
     3541 tgaaccatac cgtgcaggcc gaacgcgcgc tcatacaccc cttcttgctc ggctgtggcc
     3601 cctggcggac gttttttcgg gaccacgaga ttgctggtca gaaggaataa agcgcgcctg
     3661 cttatgctca atgtcgaatg gtataacaag ctgggggaga ttttttgcga gcggttgaga
     3721 cttgtatcaa ctttaagaag caagagcgac cggcacgcgg ggctcaatca cctctctcgg
     3781 cttcactctg cgaagccgca tgagtgggag gatattcctt cactcgccct ctggtgatag
     3841 ctcttttcgc agagactgga cacgtctgaa atgatgggag gagcaccggt tattcgatga
     3901 agatctacct ggtggatggg acctacgaac tatttcgcaa ccacttcggc gcgccgccgc
     3961 gcaaggcacc ggatgggcgc gaggtcggcg caacgcttgg cctgttacgc agcctgctgc
     4021 tgctgctcat cacgccgggc atcacccacg tggcctgcgc gttcgatcac gtgatcgagt
     4081 ccttccgcaa cggcctctac cccggctaca agaccggcgc gggcgttgac cccgacctga
     4141 tcgcccagtt cgagcttgcc gaagaggccg ttgccgcact agggctggtg gtctggccga
     4201 tggtcgagtt cgaggccgac gatgccctgg ccagcgcgac cacccgtttc aagaacaaag
     4261 ctgaggtcga acagatcgtg ctttgctcgc cggataagga tctggcccag ctggtctcgg
     4321 gcagccgcat cgttggctgg gaccgccgcc gcgccaggct catcgatgaa gcggccgtgg
     4381 tggagaaatt tggcgtgtgc ccgccgtcga tccccgactg gctggggctg gtgggcgatg
     4441 cagccgacgg cttccccggc atcccgggct ggggtgaaaa atccgcggcg gccgtgctgg
     4501 ccaaatacga gcacctcgag gcgatccccg cagatccgct cacctggggg ctcacggcca
     4561 gccgcgccgc gcggctggct gcgagcctgg cgcagcatag ggcggaggcc ctgcttttcc
     4621 gcacactggc tactctgcgc gaggatgtgc cactccagga aacgctcgcc gatctggaat
     4681 ggcagggtgc cgacgagcgg ctgaaggata tctgccagcg gctgggcgat gacaagttcc
     4741 ccgggcgggt tccgaggtgg cgccagagct gaatgtggca gcgcaaagaa gtgcagaaga
     4801 agcggaaaca cgcatgaatt gttcatgggg tgagtgatcc tgattcgttt tcgtgcagtc
     4861 agagaatttg gaagcaaggt gacctcatct ggaactgctc actcacgatg aagcaagacg
     4921 acacaataga gagaacaatc ttatcgatat gctccatttt tgaaaaaaat gctgaatgtt
     4981 aaaagtcaac taccatagga ctatgctgcg aattaaaagt gccgatcaat ttccttcctt
     5041 aattactgag tcactgtgag aaaaaaagct tcctggtcgg cttccctctc gcttttcaag
     5101 caagcgagta gcgacaatga ggcagaggat gcctgtcaac tatgcacatt agcctccggt
     5161 gcccgttgat cggtagcctg ctgcacccga aaactgcgca cttgcatcat atccagaatg
     5221 accgagtgat gcacgacccg atcaattgcc gcgaccgtgg tgagcggatt tttgaagatg
     5281 cgctcccact ccgagaacac cagattggtc gagaggcaca cactacgttg ctcatagcgg
     5341 tcactgagca aggtaaagag cacctccatc tcatcctgat catgctgcac atagccgatg
     5401 tcatcgagca cgagacaggc atagcgatgc agtttcgcca attcttgcgg caagcgcagg
     5461 tcgcgtttag cggccagcaa ccgttgcatc aactgtgccg taggcgtcca caagacagag
     5521 tgcccctgct gcaccaggtc atgggctact gcagccaaga gatggctttt agtccatcca
     5581 tcacaatgcc ctcctaggaa taagatgtcc ggcgtgttg
//
LOCUS       DASZAK010001037         5664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_383153, whole genome shotgun sequence.
ACCESSION   DASZAK010001037 DASZAK010000000
VERSION     DASZAK010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383153"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VGT44_18300"
     CDS             complement(<1..1159)
                     /locus_tag="VGT44_18300"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEV2582816.1"
                     /translation="MAEASVFQIIHLPFLLLLPTTLFPDTIATVLIGGACAFVLAYAL
                     TFAVKAFCYKFNWLDRPEPRRIHKLPVPRLGGVAIFLAFVVVALLFYSPGTNSNPTEI
                     SIFWLLLAASTLIVVVHAYDDVRGLKPLPKFIAQTIAAIIVMGPWIPTFRGVLLYYFS
                     NPFVHLNLPWFHESNIYLFMTQADFSIAAIPAVFFTWFWVTGMMNTVNLIDGLDGLAT
                     GVVGITALFVTIISVMLGQISIAVLAAVFTGAIFGFLPHNWSPAKIFMGDSGAMFLGL
                     GLAVLSIMGGAKLALALMVLGIPILDVAVVAINRIRRGQSAVHFDNSHMHYRLLATGL
                     SVKQICLLFYGLTIMFGVLALRLSHIYKFVGLALVALTMFCLILFVDRRLRQ"
     gene            complement(1287..2159)
                     /gene="prmC"
                     /locus_tag="VGT44_18305"
     CDS             complement(1287..2159)
                     /gene="prmC"
                     /locus_tag="VGT44_18305"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV2582817.1"
                     /translation="MTTTIQAALTRGEQLLSGHRTARLDAQVLLAHILGVDRSALYAH
                     PERELSPEQDLRYQNLLERRAQGEPVAYLTGHKEFFGLDLLVDRRVLIPRPETELLVE
                     AALRICRERLDAGQAPVVADIGTGSGAIPIAIAASERHLPYLYASDISEDVLEVASLN
                     CRRHHVEDRVHLLLGDLDAPLPEPVDILTANLPYISTQEESRMDEEVLRYEPSLALFS
                     EQNGLAHIERLFTNLAFSSTLLPGAAMLLEIGYAQRETVTRLLATLWPLARVSYIQDY
                     SGWDRVVQVILDVS"
     gene            complement(2163..2555)
                     /locus_tag="VGT44_18310"
     CDS             complement(2163..2555)
                     /locus_tag="VGT44_18310"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEV2582818.1"
                     /translation="MAHYFTREEAEALLPAIIPVLEQIRDQRRAMEQAESELGELHMQ
                     SRNNGHHLHERMTRVRQTLERLIEGLQGLAGQLEQFGCVLKDPDTGLIDFLSLRDSRE
                     IYLCWRLGEARILYWHYLDTGFAGRQPL"
     gene            complement(3006..4916)
                     /locus_tag="VGT44_18315"
     CDS             complement(3006..4916)
                     /locus_tag="VGT44_18315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582819.1"
                     /translation="MIILLLALIGAVIVMAILSIGRLVFGRREQFVARKFIRWFIGYF
                     IFNFIFDLAILYFGEPALTGPFGGWQWLLWPLVLSSIGNLFAVARPALISFEDLSAAA
                     QGRASSTRSSGTRIPANISRGTVAAGIFGLVAAAFIGIIVAGLIVVFTTWFDSNAKAL
                     AAIPSVTVEQPATPLVPTDPNHIVLVSQSIASFKGQQVLGSNGQNLGSSYNLDPNSYT
                     LQSINHHLYYVAPLQYNNIFINLSNSSTPGFVVVDAENPNVQPTLHVAPGDSIAYLPG
                     AILNQDLLRHVYLSGYTYGELLDPTLELDDSFHPYWTISLMQPTRGYIGDMLKQVLIV
                     DAHTGDIKVYQPQDVPPWVDRVMPSATVSQYLSWWGLYHAAPWFNPSGAGQQQPAGDP
                     QLVYNNADQPVWLVPMTSNSANDNSSTGVFLFDTHKNQAFFYPAAAGLGIGTNVQNTF
                     ASTRANILHYNVDSFQLYSICGVPTWVAIYSSNSIFQDVGILDARELNGGNVQFESTL
                     SAALTDYQTWLTTNSITNTCGATSPGGGITQTVTGKVQRISSVQSGTTTTYYIQIAGQ
                     HAIFTADLKLSPKLPLVQAGDTVTVTFLNIPNSTSATVQLKTFDDTSINLGPSGTATP
                     TATVSPTVTATP"
     gene            5198..>5664
                     /locus_tag="VGT44_18320"
     CDS             5198..>5664
                     /locus_tag="VGT44_18320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2582820.1"
                     /translation="MQLFGKKSRLDIEKLENGEDRLPPGQYLTKKWPVLSYERTPSKL
                     PDNCRLKISGAVEHPFELTWQELLALPRATITTDIHCVTTWSRYDNTWEGVHIREILK
                     LAKPLPGAKYVMAHSWTGYTTNLPLVDLDDDDVLIALKHDGEDIEDEHGGPVRL"
BASE COUNT         1260 a         1485 c         1739 g         1180 t
ORIGIN      
        1 gctggcgcag gcgacgatcg acgaagagga tcaggcagaa catggtcagc gcgacgagcg
       61 ctaacccgac gaatttgtag atgtgcgaga ggcgcagggc cagtacgccg aacatgatgg
      121 tcaggccgta gaagagcagg cagatctgct tgacgctcag cccggttgcc agcaagcgat
      181 aatgcatgtg ggaattgtcg aagtgaacgg cggattgccc gcggcggatg cggttgatgg
      241 ccacgacggc tacgtcgagg atggggatgc ccagcaccat caaggccagc gccagtttcg
      301 ccccgcccat gattgagagg actgccaggc ccagacccag gaacatcgcg ccgctatcgc
      361 ccatgaagat tttcgcgggg ctccagttat gtggcaggaa gccgaagatt gcaccggtga
      421 agacagctgc cagcaccgca atactgatct gcccaagcat gacgctaatg atggtgacga
      481 agagcgcggt gatgccgacc acgccggttg ccagcccatc caggccgtcg atcaggttga
      541 cggtgttcat catgccggtt acccagaacc aggtgaagaa gacggctggt attgcggcaa
      601 tgctgaaatc ggcctgggtc atgaacaggt agatgttgga ttcatggaac cagggtaagt
      661 tcaggtgaac gaacgggttg ctgaagtagt agagcaggac accgcgaaat gtgggtatcc
      721 agggacccat gacgatgatg gcggcaatag tctgcgcgat aaatttgggc agcggcttta
      781 acccgcgcac atcgtcgtaa gcatgcacga ccacgatcaa tgtggaggca gccagcagca
      841 accagaagat ggagatttcg gtcggattag aattggttcc aggcgaatag aagagcagcg
      901 ctaccaccac gaacgccagg aagatggcca cgccgcccag gcggggaact ggcaatttat
      961 ggatgcgccg gggttctgga cgatcaagcc agttgaattt atagcaaaaa gccttaaccg
     1021 cgaaggtcag cgcatacgcc agcacaaagg cgcaggcccc accgatcagc acagttgcaa
     1081 ttgtgtctgg aaacagcgtt gtaggaagca gcaggagaaa tggtaggtga ataatttgaa
     1141 agacgcttgc ctctgccata gaatacttcc ttttgctatg cagaacggac tatacatata
     1201 agacgaataa ggtttgagaa agtgaacatg ctctaatcta attgcacagg ttctgtttta
     1261 gagaacgtat acgccggttg attttatcat gagacatcta gtattacctg taccacccta
     1321 tcccatcctg aataatcctg tatatacgac acgcgcgcta atggccagag cgtcgcgagc
     1381 aggcgcgtca ctgtctcgcg ctgcgcgtac ccaatttcga ggagcatggc agctcctggt
     1441 aatagtgtac ttgaaaaagc aagattcgta aacaagcgtt cgatgtgagc cagcccattt
     1501 tgttcgctaa agagggccag gctcggctcg tagcgcagaa cctcctcatc catccgcgat
     1561 tcttcttgcg tgctgatgta gggcaggtta gccgtcagta tatcgaccgg ctcaggcaag
     1621 ggtgcgtcca ggtcgccgag gagcaggtgg acgcggtcct cgacgtggtg cctgcggcaa
     1681 ttgagcgagg cgacctccag gacgtcttca gagatgtcgc tggcgtacag gtagggtaga
     1741 tgccgttcgc tggcggcgat ggcgatgggg atggccccgc tgcctgtgcc gatgtcggcg
     1801 acgacaggcg cttgcccggc gtccaggcgt tcgcggcaga tgcgcagcgc cgcctcgacc
     1861 agtaattcgg tttccggtcg ggggatcagc acacgccggt ctaccagcaa gtcgagtccg
     1921 aaaaactcct tatgccctgt caggtaggcg accggttcgc cctgtgcgcg tcgttcgagc
     1981 aagttctgat aacgtaggtc ctgttcaggt gataactccc gctctggatg ggcgtagagg
     2041 gcgctgcggt caacaccgag gatatgcgcg agcaagacct gggcatcgag ccgggctgtg
     2101 cgatgcccgg agagcaactg ttcgccgcgc gtgagggctg cctggatggt ggtcgtcata
     2161 gcctagagtg gctggcggcc cgcgaagccg gtatcgaggt agtgccagta gagaatacgc
     2221 gcttcaccga gccgccagca gaggtagatt tcgcgactat cgcgaagact caggaagtcg
     2281 atcaagcccg tgtcggggtc cttgagtacg cagccgaact gttctaactg acctgccagg
     2341 ccctgcagtc cctcgatcag ccgctccaac gtctgtcgca cgcgagtcat gcgttcgtgc
     2401 aggtgatgtc cattattcct ggactgcatg tgcaattcgc ccaactcgct ctccgcctgc
     2461 tccatcgcgc ggcgctggtc gcgaatctgc tctaataccg ggatgatagc cggaagcaaa
     2521 gcctcggcct cttctcgcgt gaaataatga gccatatctt ttctccgctc gcactacccg
     2581 ctattcgttg cctttttcca agccccggtt ttctcccttt tccatcccct cttgcagtat
     2641 ttcctggtag acccaggagt cttctaaaaa gtttatattc atggcaaaca tcctcaatca
     2701 acgaaacgaa atcctgggga tgctctcgaa agagccgctt gatcgaatca tcccacactt
     2761 cagccataag cgctacgaat ccttcccacc ttgatggaac aagtgtagca tacctattga
     2821 tcgttgtcga gaagcactgg tagagttttt caggatttgt aaatgattaa tcagggttcc
     2881 tgaatcgcga ttacgcactc tcgtaatcgc gattcaggaa ccccatcgat accgttcaat
     2941 caacgggtta cggcgggcga cgcaagcgtc cccaccgtcc ctcatcatcc tctcccgacc
     3001 ctacgtcatg gtgtggctgt cactgttggc gacaccgttg ccgttggtgt ggctgtaccc
     3061 gacggcccaa ggttgatgct tgtgtcgtcg aaggttttga gctgcaccgt tgcgctggtg
     3121 gagttgggga tgttcagaaa ggtgacggtc acggtgtcac cggcctgtac gagcggcagc
     3181 ttgggggaga gtttcaggtc ggcggtgaag atggcgtgct gccccgcaat ttggatatag
     3241 taggttgtgg tggtacccga ttgcacagat gagatgcgtt gcaccttgcc cgttacggtc
     3301 tgggtgatgc ccccgcctgg cgaggtggcc ccgcaggtgt ttgtgatcga gttggtggtg
     3361 agccatgttt gatagtcggt cagcgcggca gagagggtcg actcgaactg cacattgccg
     3421 ccgttgagtt cccgggcatc caggataccc acgtcctgga agatgctgtt gctgctatag
     3481 atggcgaccc aggtggggac accgcagatc gagtagagct ggaagctgtc cacgttgtag
     3541 tgcaggatat tggcacgcgt cgatgcgaag gtattctgta cgttggtgcc gatgccgaga
     3601 ccggcggcag ccgggtagaa gaatgcctgg tttttgtgcg tgtcgaagag aaagacgccc
     3661 gtgctggagt tgtcgttggc cgagttcgag gtcattggga cgagccagac gggctgatcg
     3721 gcattgttgt agaccagttg cggatcgccg gcgggctgct gctggcccgc gccggatggg
     3781 ttaaaccagg gtgcggcgtg gtacagaccc caccaggaca ggtactggct gacggtggcg
     3841 gatggcatga cgcggtccac ccacggtgga acgtcctgcg gctggtagac tttgatgtcg
     3901 ccggtgtgcg catcgacgat caagacctgt ttcagcatat cgccgatgta cccgcgcgtg
     3961 ggctgcatga gcgagatggt ccagtaggga tggaagctat cgtcaagctc caaggtgggg
     4021 tcgagcagtt cgccgtaggt atagccgctc aggtagacgt ggcgcagcag atcctggttg
     4081 aggatcgcgc cgggcaggta ggcgatggag tcgcctggag cgacgtgcag cgttggctgg
     4141 acattcggat tctcggcatc cacgacgaca aagccaggtg tggatgaatt tgataggttg
     4201 atgaagatgt tgttgtattg cagtggcgca acgtagtaaa ggtgatgatt gatcgactgc
     4261 agcgtgtagc tgtttgggtc gaggttgtag gatgagccga ggttctggcc gttggaacca
     4321 agcacctgct gccccttgaa gctggcgatg ctctggctga ccagcacgat atggttgggg
     4381 tcggttggca cgagcggcgt cgcaggctgc tcgacggtga cgctggggat ggcggccagc
     4441 gctttcgcgt tgctgtcgaa ccaggtggtg aagacgacaa tcagcccagc gacgatgatg
     4501 ccgataaaag ccgctgctac caggccaaag atacctgccg cgaccgtacc gcgcgaaatg
     4561 ttggcgggaa tgcgtgtacc ggatgaacgg gtcgagcttg ctctgccctg tgcagcggct
     4621 gagagatcct cgaagctgat cagcgcgggc cgcgcgacgg cgaacaggtt gccgatgctg
     4681 ctcaagacga gcggccagag cagccactgc caaccaccga acggtccggt cagcgcaggt
     4741 tcgccgaagt agaggatggc aagatcgaag atgaagttga agatgaagta gccgataaac
     4801 cagcgaatga acttgcgcgc gacgaactgt tcgcgtcgcc cgaagaccag tcgcccgatg
     4861 ctcagaatcg ccatgacaat caccgcgccg atcagcgcga gcaacagaat aatcataacc
     4921 gtctctcttt ctaactatag cgctatgcaa taatgacgga atatcttccc ctattgtagt
     4981 gttgttacgg gtaatacgca actaccaatt ctggacgcaa agatggagca gaatcccaat
     5041 aatcgcgtcc ctacgacggg atgtaacatt tcgaggctcg ggttgtctat acatcaacgc
     5101 ctacccattg cggatcatca tcgtcttcct gttcaatgta ctatataata ctgttagccc
     5161 tgttcgccgt tcagaacgca aaggagtcat caagatcatg caactgttcg gaaaaaaatc
     5221 gagactggat atagaaaagc tggagaatgg agaggatcgc ctgcctcccg gacaatatct
     5281 gacgaagaag tggccggtgc tgagttacga gcgcacgccg agcaagcttc ccgataactg
     5341 tcgcttgaag atcagcggcg cggttgagca tccctttgaa ttgacctggc aggagctgct
     5401 ggcgctgccg cgcgcgacca ttacgacgga tatccactgc gtcacaacgt ggagccgcta
     5461 cgacaatacg tgggagggtg tgcatatccg cgaaatcctg aagctggcga agccactgcc
     5521 cggcgcgaag tatgttatgg cgcattcctg gaccggctac acgaccaatc tgcccctggt
     5581 cgacctggac gacgacgatg tcctgatcgc cctcaaacac gacggcgaag atatcgaaga
     5641 cgaacatggc ggcccggtgc gcct
//
LOCUS       DASZAK010001038         1778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_61521, whole genome shotgun sequence.
ACCESSION   DASZAK010001038 DASZAK010000000
VERSION     DASZAK010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61521"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VGT44_18325"
     CDS             <1..332
                     /locus_tag="VGT44_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582821.1"
                     /translation="LARSAVEVLVCYRHDSVLPIPVVELESGNTDRPHEFPHVFTSYE
                     EAYHSRSKTGRLTIVGGLLCGVQRAYDLLETLPESTRRKYETSAHQYQKRRRGRQLAL
                     MPGAEAI"
     gene            633..1139
                     /locus_tag="VGT44_18330"
     CDS             633..1139
                     /locus_tag="VGT44_18330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582822.1"
                     /translation="MTTRTEMTLPAGTALVETFDGRWFPAFVPSEENPLRWVHLIDGP
                     LDIPPALDGDPEAGYQMRAEAIAACWAWQEAAVLPFEWEAVARQIEVYPERAAWYIDE
                     IAQLTGNTRQVQRSGPFASVSVAQPSDQETTITAHGANPDEAIENLYQCVYEWACPQQ
                     MLPLQRAS"
     gene            1237..1455
                     /locus_tag="VGT44_18335"
     CDS             1237..1455
                     /locus_tag="VGT44_18335"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2582823.1"
                     /translation="MRKNTNRHKLDEPFPSAEVKKLLKVGDVADILGVSPVKVYDLLK
                     NGLPSVKIDGARRIQPDKLQAWIEQHCA"
     gene            1530..>1778
                     /locus_tag="VGT44_18340"
     CDS             1530..>1778
                     /locus_tag="VGT44_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582824.1"
                     /translation="MMQAPHDDLSTRKLPALEELQARPQWVCWRKEKRQGKPTKVPYS
                     AMTGKRAETDNPATWASYAQAVQAQRARRYDGIGYVFHQ"
BASE COUNT          412 a          533 c          530 g          303 t
ORIGIN      
        1 acctggcgcg ctcggcggtg gaggtgctgg tgtgctaccg gcatgatagc gtcctgccca
       61 tccccgtcgt ggaactggag agcggcaaca ccgaccgccc gcatgagttc ccccatgtgt
      121 tcacctcgta tgaggaggcc tatcactccc gctccaaaac gggccggctg accatcgttg
      181 gcggcctgtt gtgtggcgtg caacgggcct atgatctgct cgaaaccctg ccggaaagca
      241 ccaggcgcaa gtatgagacc agcgcccacc agtatcagaa gcgcaggcgc ggcagacagc
      301 tggcgctcat gccaggcgca gaggcgatat agccaggaat gcctcggata tcgcccttcg
      361 acccacgtca atagaacagg catgacaggc gaggcttctc ctgcgcgcat tcaggaacat
      421 caccccgatc actggaggcg tttcccaaag ggaggtcgaa cagtgcgcga ttggcagcga
      481 gattggcaca ggattgcgac gctcccatga gcagcggcgg ctcacagcgg tcgaaaacaa
      541 gaagcgaaga gcaatcaagc agaccaggag caatgtccct gatctgctct ttttgtccag
      601 aaggaacagg tttcaatgag gagaacacag caatgacaac gagaacggaa atgacgctcc
      661 cggcaggcac ggcgctggtc gaaaccttcg atgggcgatg gtttcctgcc tttgtcccct
      721 ctgaggagaa cccgctgcgg tgggtgcatc tcattgatgg gccgctcgat atccctccag
      781 cgcttgacgg agatccagag gctggatacc agatgcgagc ggaagcgatc gctgcctgct
      841 gggcctggca ggaagcagcc gtccttccct tcgagtggga ggcagtggca agacagatcg
      901 aggtctatcc ggagcgcgcg gcctggtata tcgatgagat cgcgcagttg accggaaaca
      961 cgcggcaagt acagcgctct ggccccttcg cctcggtgag cgttgctcag ccatctgacc
     1021 aggagacgac tatcaccgcg catggcgcaa accccgatga ggcgatcgag aacctctacc
     1081 agtgtgtgta cgagtgggct tgcccgcagc agatgctccc cctccaacgc gccagctagc
     1141 tcacactcca cagcaacaga cccagatgag cagggacgac cttgccagcc tgggctgttt
     1201 gtttttcatc tcactattgg aaggaagtaa agaaccatga ggaagaacac gaacagacac
     1261 aaactggatg aaccttttcc ctccgctgag gtgaagaagc tgctcaaggt gggtgacgtt
     1321 gctgacatcc tgggcgtcag tccggtcaag gtctacgacc tgctgaaaaa tggcttaccc
     1381 tcggtcaaga ttgacggcgc gcgccgcatt caaccggaca aactgcaagc ctggatcgag
     1441 cagcattgtg cgtgacctgc tccgccgcgt gagtccatct cagcccaccg gctcaccgca
     1501 tgaccagcac tggaaggaag gcctgaggaa tgatgcaagc accgcatgac gatctatcca
     1561 cgcgcaagct ccccgccctg gaagaactgc aagcgcgtcc ccagtgggtc tgctggcgca
     1621 aagagaaacg ccagggcaag cccacgaaag taccctacag cgcgatgacc ggcaaacgag
     1681 cggaaactga taaccctgcg acatgggcca gctatgccca ggcagtccag gcgcagcgtg
     1741 ccaggcgcta tgatgggatt ggctacgtgt tccaccag
//
LOCUS       DASZAK010001039         1695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_702141, whole genome shotgun sequence.
ACCESSION   DASZAK010001039 DASZAK010000000
VERSION     DASZAK010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702141"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VGT44_18345"
     CDS             complement(<1..359)
                     /locus_tag="VGT44_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HEV2582825.1"
                     /translation="MGRVWMLGDNVNTDVIIPGRYNVTTDRAQLARYCLCEVMPEFAQ
                     QVQAGDVLMAGHNFGCGSSREHAPAAIQACGVRVVIARSFARIFFRNAVNIGLPVLVC
                     EEAAPASAAGQEIEVDLA"
     gene            complement(385..1635)
                     /locus_tag="VGT44_18350"
     CDS             complement(385..1635)
                     /locus_tag="VGT44_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEV2582826.1"
                     /translation="MGTLTEEIFSHKLGRAVEQGETVVVDVDRVMSHDTTTPLAIQAF
                     RKMADLTGGRVFDARRSHIVFDHIIPAATIAAATLHREVRSFAQEQDISILQEGICHQ
                     VMPERGYVIPGDIIVGADSHSCTYGALGAFATGMGSTDIGVAYATGRAWFRIPQTINV
                     RLEGALPAGVYTKDVSLEIVRHLGVDGANYRAIEYTGSFIDSISVSERFTLCNMAIEM
                     GGKAGLIAADETTRAWLRGRTEREYPMLAPRHPVYEQVVEIDVTALQPLVACPPDVNN
                     LAPVSEVEHIEIDQVFLGTCTNGRLDDLAIAANILRGKTIHPRTRMVVIPASREVYLE
                     AMRLGYLEIFAQAGASVGTPGCGPCIGRHFGVLAPGERALTTMNRNFTGRMGDPTAEI
                     YLGSPATVAASALTGHITDPRNFV"
BASE COUNT          316 a          529 c          535 g          315 t
ORIGIN      
        1 gccaaatcga cctcgatctc ctgcccggcg gcgctggccg gcgctgcttc ttcgcagacc
       61 agcacgggca ggccgatgtt gacggcgttg cgaaagaaga tacgcgcgaa actgcgcgca
      121 atcacgacgc gcacgccgca cgcctggatc gcggcggggg catgttcgcg cgacgaacca
      181 caaccgaagt tgtgacccgc catcagcaca tcgcccgcct gcacctgctg cgcgaattcc
      241 ggcatgacct cgcacaggca atagcgcgcc agttgcgcgc ggtcggtagt gacgttgtac
      301 cgtcccggaa tgatgacatc ggtgttgacg ttatcgccaa gcatccatac acggcccatt
      361 gttgttgatt cctttcattg tgtcctatac gaaattgcgc ggatcggtga tgtggccggt
      421 cagggcgctg gcggcgacgg tggccgggct gccgaggtag atctcggcgg tgggatcgcc
      481 catgcgcccg gtgaagttgc ggttcatggt tgtcagggcg cgctcgccgg gggcaagcac
      541 gccaaaatgc cggccaatgc aggggccgca gccgggcgta ccgaccgacg cgcccgcctg
      601 ggcgaagatt tccaggtagc ccaggcgcat cgcctcaagg tacacctcgc gggaagccgg
      661 aatgaccacc atgcgcgtgc ggggatggat ggtcttgccg cgcaggatgt tcgcggcgat
      721 ggccagatca tcgagtcgcc cattcgtgca ggtgccgaga aaaacctggt ctatctcgat
      781 atgctcgacc tcgctgaccg gggcgagatt gttgacatcg ggcgggcagg caacgagggg
      841 ctgtaacgcc gtcacgtcga tctcgaccac ctgctcgtaa acgggatggc gcggcgcaag
      901 catggggtat tctcgctcgg ttcgcccgcg cagccaggcg cgcgtcgtct cgtcggcggc
      961 gatcaggccg gccttgccgc ccatttcaat cgccatgttg cagagcgtga agcgctcgct
     1021 gacgctgatc gagtcgatga acgagccggt atactcgatc gcgcggtagt tcgcgccatc
     1081 gacgcccagg tgacgcacga tctccaggga gacatctttg gtatacacgc ccgccggaag
     1141 cgcgccttca agacgcacat tgatcgtctg cgggatgcga aaccaggcac gcccggtggc
     1201 ataggccacg ccgatgtcgg tcgaacccat acccgtcgcg aatgcgccca gcgcgccata
     1261 cgtgcagctg tggctgtcgg cccccacgat gatatcgcca ggaatgacgt agccgcgctc
     1321 cggcatgacc tggtgacaaa tgccctcctg gagtatcgag atgtcctgct cctgcgcaaa
     1381 gctgcgcacc tcgcgatgca gcgtggcagc cgcaatcgtc gcggcaggga tgatgtgatc
     1441 gaagacgata tgcgacctgc gcgcgtcaaa gacgcgcccg ccggtcaaat ccgccatttt
     1501 gcgaaatgcc tggatcgcca ggggcgttgt ggtatcgtgg ctcatgacgc ggtcaacgtc
     1561 caccacgacc gtctcgccct gttcaacggc gcgacctaac ttgtgactga agatttcttc
     1621 ggttagcgtt cccatacgag atggctcctt tcctgttgcc agatgagaat aaaactatcg
     1681 acttcatcct gtttc
//
LOCUS       DASZAK010001040         3539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_702148, whole genome shotgun sequence.
ACCESSION   DASZAK010001040 DASZAK010000000
VERSION     DASZAK010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702148"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VGT44_18355"
     CDS             complement(<1..203)
                     /locus_tag="VGT44_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582827.1"
                     /translation="MSASQWFDGIPVIGKLPPEQAVEKLREVGEDEIADMLEVQQGSA
                     AKSFEIAKRGWWPFPAREKPWLHT"
     gene            598..2118
                     /gene="mptA"
                     /locus_tag="VGT44_18360"
     CDS             598..2118
                     /gene="mptA"
                     /locus_tag="VGT44_18360"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00294.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HEV2582828.1"
                     /translation="MSIDLNYMQPISHNGKTPVVTHTIFLALGSNLGDRRANLAAALQ
                     RLRNAVEISDISSVYETEPVGYLDQPRFLNMVLRGHSSLSPEALLAYVKTIEATLGRQ
                     ATFRNGPRLIDIDILFYDDLLDVQEHLSIPHPRFSERAFVLAPLAEIAPDVIDPASGK
                     SAQQLLDAVSQDGVQRLDNGLRISLEHDIQSGKPAVHVKLGRTGVTGITKAILIGNEG
                     HQQWFNATFGLYAELDSAQAGVHMSRFSDALDDVLEETVRESWSHLELLAESLAKRIV
                     EKQHARRAEVHIRTTLPVQRWTPVSGRPTQEIYGMLAEAVATPDYTRRMIGVEVEGMV
                     ACPCAQDMVHSYAAVRLQEEGFPKNVIEKMLSVTPLATHNQRGRATLLIGTDSSNGAE
                     HHIDARDLIDLAESAMSSENYALLKRPDELYIVNKAHAHPQFVEDVARELLRSAIERY
                     HELPDESYLWVHQRNEETIHKYDVEAEGWGTLGELRSEILQNIPVARHTSKEAWLG"
     gene            2220..2579
                     /gene="rsfS"
                     /locus_tag="VGT44_18365"
     CDS             2220..2579
                     /gene="rsfS"
                     /locus_tag="VGT44_18365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV2582829.1"
                     /translation="MEGVLSDPVQLARAAADIAYDKKASDILVLDIKDISSFADYFVI
                     CSGNNTRQITAIADALDEELDKQGARVLHREGTSESGWLLLDFGDVIVHIFGLKEREY
                     YRLEHLWNEAKTVVYLQ"
     gene            2603..2785
                     /locus_tag="VGT44_18370"
     CDS             2603..2785
                     /locus_tag="VGT44_18370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582830.1"
                     /translation="MTFFRNRMFLEGIAIGGICGIVIGSIIAFTLGESSIENLRKVIN
                     KRVPGRGRVPFEYLSQ"
     gene            2904..>3539
                     /locus_tag="VGT44_18375"
     CDS             2904..>3539
                     /locus_tag="VGT44_18375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582831.1"
                     /translation="MSVTASPGFAASLTPTCAPLAPPPVPGSPVSPPATPGIVVFNEI
                     LTNPGSNWNCAEQGGAYTIMSDSWVELYNPQNQPFNLYEVHAYIDDGPNTFRFYLPFG
                     AAIAPHGFLVVFPNSAANSTLLAGSNLRLMFATSSAAIDEVSFPGLAVNDAYARIPDG
                     SPTWEITTTPTIDSSNIETSSIKATPTTSSSGSSSSGGLSSSGGSTQGTTTP"
BASE COUNT          796 a         1022 c          961 g          760 t
ORIGIN      
        1 gtgtgcagcc agggcttctc tcgcgcggga aaaggccacc agcctcgctt cgcaatctcg
       61 aaagactttg ccgcgctgcc ctgctgcacc tccagcatgt cggcgatttc atcctcaccg
      121 acctcccgca gcttctcgac agcctgctct ggcggcagct tcccaatcac cggtatacca
      181 tcaaaccact gcgatgcgct catacactta tagcctcact tgcttgattt ctttgtatga
      241 tttgcactat atcattctat gtatgcaaaa tcaatgatgc attacccgta acacagggta
      301 tgtaaatgat tgctgaggtg cgcggagggc gaccacctac tctcgtgtac atactagggg
      361 caacttatga tgagtgttca ggaccacgat cgtcacccac gtctgtcatc ctgagcgcag
      421 cgaaggatct cggcgtccag agcataagac ccttcgctgc gctcagggtg acattacccg
      481 accgatcctg gtcctgggaa cttataataa gtaagtacca atgggatgaa acagaccacc
      541 gacgctgttc tatactatgg atatgcatca aaccagtagt gaaaggtatt attgtgcatg
      601 agcatagatt tgaattatat gcagccgatt tcccacaatg ggaaaacgcc ggttgttacc
      661 catacgattt ttcttgccct gggttctaac ctgggcgatc gccgcgccaa cctggcagcg
      721 gcgctccaac gcctgcgtaa cgctgtcgag attagcgata tctcgtccgt gtatgaaacg
      781 gagccggttg gctatctcga tcaaccgcgt ttcctcaata tggtgctgcg cgggcattcg
      841 tcgttgagtc ctgaagcgct gctggcctac gtcaagacga tcgaggctac gctgggccgg
      901 caggcgacct tccgcaatgg gccgcgcctg atcgacatcg atattctgtt ttacgacgac
      961 ctgctcgatg tgcaggaaca tctcagcatc ccgcacccgc gtttcagcga acgggcgttt
     1021 gtgcttgctc cgttggcaga aatcgcgccg gatgtgatcg accccgcgag cggcaagagc
     1081 gcgcagcaat tgctcgacgc ggtttcgcag gatggggtgc agcggctgga caatggcctg
     1141 cgtatctcgc tggaacacga tatccagagc ggcaagccag ccgtgcatgt caagctggga
     1201 cgcacgggag tgaccggcat caccaaggcc atcctgattg gcaatgaggg ccaccagcag
     1261 tggttcaacg ccacgttcgg cctgtatgcc gaactggatt cagcccaggc aggcgtccac
     1321 atgtcgcgtt tcagcgacgc cctggatgat gtgctggaag agacggttcg cgaatcctgg
     1381 tcgcaccttg agctgctggc ggagagcctg gcgaagcgca ttgtcgaaaa gcagcacgcg
     1441 cgccgcgccg aggtgcatat ccgcaccacc ttgcctgtgc agcgctggac gccggtctcc
     1501 ggcagaccca cccaggagat ctacgggatg ctggcagaag ccgtcgcgac gccggattac
     1561 acgcgccgca tgatcggcgt cgaggtcgag ggcatggtgg cctgtccctg cgcccaggat
     1621 atggtgcatt cctacgccgc cgtgcgcctg caagaggaag gcttccccaa aaatgtcatc
     1681 gagaaaatgt tgagcgtgac gcccctggcg acccacaacc agcgcgggcg cgccacgttg
     1741 ttgatcggca ccgatagctc aaatggcgcg gagcatcaca tcgacgcgcg cgacctgatt
     1801 gacctggcgg aaagcgccat gagttcggag aactacgccc tgctgaaacg ccccgatgaa
     1861 ctctacatcg tcaacaaagc ccacgcccac ccacaatttg tcgaagatgt ggcgcgcgaa
     1921 ctgctgcgct ccgccatcga gcgctatcac gagttgcccg acgagagcta cctctgggta
     1981 caccagcgca acgaagaaac catccacaag tacgacgttg aagccgaagg ctggggtacc
     2041 ttaggtgaac tacgctccga aattttgcag aatattcccg tcgcacgtca tacaagtaag
     2101 gaagcgtggc tggggtgaca ctcttgcctg accatgctat aatgcgggta caatcagtaa
     2161 agaacaacag gaacgtgcat caaaacgttt gtctataata aagagtatag ggagcatttg
     2221 tggaaggagt cctttcagat ccggttcagc tggcgcgcgc ggcggctgat attgcctatg
     2281 acaagaaagc ttcagatatt cttgtgctgg acatcaagga tatctcgagc ttcgcagatt
     2341 actttgtcat ctgcagcggg aataatacgc ggcaaatcac tgcaattgcc gacgcgcttg
     2401 acgaagaact ggataaacag ggagccaggg tactgcatcg cgagggcact tcggaatcgg
     2461 gctggttact cctggacttt ggagatgtca tcgtacacat cttcgggtta aaggaacgcg
     2521 aatattaccg gctggaacat ctctggaatg aagcaaagac ggtcgtctac ctccaataaa
     2581 ttaaaagaga aggtgatcaa cgatgacatt ttttcgtaat cgcatgtttc tcgaaggaat
     2641 agccatcggg ggcatttgtg gcatcgtgat tggctccatc atcgctttta cgcttggcga
     2701 aagcagcata gagaacctgc gcaaggtcat caacaagcgc gtaccggggc gcggtagagt
     2761 cccgtttgaa tatctgtcgc aataaataac tgtgcggctg cgagccggag ggggccagtg
     2821 tgcaggcgag gtcagacgtt gctcggcgta tatcatatgg ttgttttgcc tgtctctccc
     2881 tcgtccttgc gttagccgcc ctcttgagcg taaccgcttc accgggcttc gcagccagtc
     2941 taacgccaac ctgcgcgcct ctcgcgcctc cacccgttcc aggttcgcct gtttccccgc
     3001 ctgccacacc gggcatcgtt gttttcaacg agattttgac caatcccggc tctaactgga
     3061 attgcgccga gcagggtggg gcgtatacga tcatgtccga ttcctgggtc gagctgtata
     3121 acccgcaaaa ccagccgttc aacctgtacg aagttcacgc ttacatcgat gatggcccaa
     3181 acacctttcg attctacctt cccttcgggg cagccatcgc accacacgga ttcctggtcg
     3241 tcttcccaaa tagcgcggcg aactctacgc tgctggcggg cagcaacctg cggctgatgt
     3301 tcgcgacatc gagcgcggcc attgacgagg tcagttttcc cggcctggct gtcaacgatg
     3361 cctacgcgcg cattcccgac ggctccccaa cctgggaaat taccacgacg cccaccatcg
     3421 actccagcaa catcgagaca tcttccatca aagccacacc cactacttct tcgagcggtt
     3481 caagtagttc tggcggatta agcagttcgg gtggctctac gcagggaacg acaacacca
//
LOCUS       DASZAK010001041         2532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_99310, whole genome shotgun sequence.
ACCESSION   DASZAK010001041 DASZAK010000000
VERSION     DASZAK010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99310"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VGT44_18380"
     CDS             complement(<1..294)
                     /locus_tag="VGT44_18380"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582832.1"
                     /translation="MSTSPKASTASTAVLSSRLLGGRAPRWITIILVVAVILIFFSVA
                     NDEWLTIANYTLIAAIAALSLNVLSGYTGQVSLGIAFFMAIGAYTTAYLGGNVP"
     gene            complement(291..1190)
                     /locus_tag="VGT44_18385"
     CDS             complement(291..1190)
                     /locus_tag="VGT44_18385"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2582833.1"
                     /translation="MAYFLQLVFAGIALGCIYALIGLGFSIIFKASEVINFAQGELLL
                     VGAYVVSSGVFNWHLNFLVSVLLGVVVTVLIGLLFERFVLQRMIGRPVFSILMITIGL
                     DTILRTGVIVGWGSNPLPAAAPYTVTSGFNISGVHLGANDLLTIIVTIVLCAMLFYFF
                     QYTRYGLAMRATALDQEAALAVGINIRTVYALAWGIAAAIATIGGVFLAIGSFAMDPT
                     LGGAALLAFPAIILGGIDSITGAVVGGIIIGLVQDLTAGYESHVASFLGAGIHQITPY
                     LIMILVLLIRPYGLFGTRKVERI"
     gene            complement(1206..2072)
                     /locus_tag="VGT44_18390"
     CDS             complement(1206..2072)
                     /locus_tag="VGT44_18390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2582834.1"
                     /translation="MSVAPTGGQQLAQPTAPLLTVEDISVRFAGVKALTDVSLQVRSG
                     ELFAVIGPNGAGKTSFFNVLSRVYQPVTGRVTFDGDDLLRLKPHQVARVGIARTFQNL
                     GQFPTMTVLDYLLLGRHTRMRSGILLGGVYLGPARSEEKRNRAYCQHLLDLLGLQRMQ
                     NHALGSLPYGLQKRADIARALALQPKLLLLDEPVAGMSIEETEDIAVVILDIKGQLGV
                     TQVLVEHDMALVMGIADRVLVLDFGRVIAQGTPAVVQANPDVIKAYLGEEFGEKVADT
                     VSIPTEDGRSIP"
     gene            complement(2144..2532)
                     /locus_tag="VGT44_18395"
                     /pseudo
     CDS             complement(2144..2532)
                     /locus_tag="VGT44_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          619 a          747 c          685 g          481 t
ORIGIN      
        1 cggcacgttg ccaccaagat aagcggtagt gtacgcgcca atcgccatga agaacgcgat
       61 acccagcgaa acctgccctg tatatcccga aagcacgttg agcgaaagcg cggcaatcgc
      121 cgcgatcagc gtatagttgg caatcgtgag ccattcatcg ttcgccaccg agaagaagat
      181 gagaatgact gctaccacca gaataatggt gatccagcgc ggcgcccttc ctcccaggag
      241 tcgtgatgat agtacagcag tggatgcagt gctggccttt ggagatgtgc tcatatgcgc
      301 tccacttttc tggttccaaa aagcccgtaa ggccgaatga gcagtacaag aatcatgatc
      361 aagtagggag taatctggtg aatacccgcg cccaggaagc tggcgacgtg gctttcgtag
      421 ccggcagtca ggtcctgcac caggccaata ataattccac ccacgacggc cccggtaatc
      481 gagtcaatac cgcccaggat gatggccggg aaggccagca gtgccgcgcc tcccagcgtc
      541 gggtccatag caaaggaacc aatagcaaga aaaaccccgc cgatggtggc gatggcggcg
      601 gcgatacccc atgccagcgc ataaaccgtg cgaatgttga tacccaccgc cagcgcagct
      661 tcctggtcga gcgcagtagc gcgcatggcc agtccatagc gggtgtactg gaagaaataa
      721 aagagcatgg cgcacaaaac gatagtcacg ataatcgtca gcaggtcgtt cgcgcccagg
      781 tggacgccgc taatgttaaa gccgctcgtg accgtataag gagctgctgc cgggagagga
      841 ttcgatcccc atcctacgat aacgcctgtg cgcaggatcg tatccagccc aatcgtaatc
      901 atcaaaatgg agaagaccgg gcggccaatc atgcgctgca gcacaaagcg ttcaaagagg
      961 aggccgatca gcacggtgac gacaacaccc aggagcactg agacgaggaa gttcaggtgc
     1021 caattgaaca cccccgacga aacaacatag gctcctacca gcagcagttc accctgtgcg
     1081 aagttaatga cctcgctggc tttgaagata atagaaaaac cgagcccgat gagtgcatag
     1141 atacagccca gcgcaattcc agcaaagacc agttgaagaa aatacgccat gttccctcct
     1201 gtaagtcacg ggatcgagcg gccatcctcg gtcgggattg acacagtatc cgcgactttc
     1261 tctccaaact cttcacccag gtaggcttta atcacgtcag ggttggcctg aaccacggcc
     1321 ggcgtcccct gcgcgatcac gcgcccgaaa tcgaggacca gcacgcggtc agcaataccc
     1381 atgacaagcg ccatatcatg ctcgaccaga acctgggtaa caccaagttg ccctttgata
     1441 tccaggatga caactgcaat gtcttcggtc tcctcgatgc tcatgcccgc caccggctcg
     1501 tccagcagga gcagcttcgg ttgcaaggca agtgcgcggg caatgtcggc gcgcttttgc
     1561 aagccatagg gcaggctccc cagcgcgtgg ttttgcatgc gctgcaatcc caacaggtcg
     1621 agcaagtgct ggcagtaggc gcggttcctt ttttcttcgc tgcgcgccgg gccgagatag
     1681 acgccgccga gcaggatgcc actgcgcatg cgcgtatgcc gacccagcag cagatagtcc
     1741 agcaccgtca tggtagggaa ctgtccaaga ttctggaagg tacgcgcaat gcccacgcgc
     1801 gctacctggt gcggttttag ccgcagcagg tcgtctccat caaaagtcac ccggcctgtc
     1861 acaggctggt agacccgcga taacacgttg aagaacgatg ttttcccggc gccattaggg
     1921 ccgatcacgg cgaacaattc tcccgacctc acctgcaagg agacatccgt cagcgctttg
     1981 acaccggcga aacgtaccga aatatcctca acggtgagta aaggtgcagt gggctgtgcg
     2041 agttgctgac ccccagttgg agcaacgctc atatggccca tctcctttca ggtcgccgct
     2101 ggcgaatcgt tcccatgttg ctccgttccc ctccctcgtg caatcctaaa taaaacttgc
     2161 gtacgctttc gtcctgcaag agttgggggg cgggttgcgc cagcaccact ttaccggttt
     2221 ccagtacata gccataatga gcaaccgaga gtgccatctg cgcattctgt tctaccagta
     2281 atacggcagt tccaccggca tttatctccg aaataatgga acgaatttgt tgtaccatga
     2341 agggagccaa tccgagcgag ggttcgtcca gcaagagcaa cttcgggttt gacatcagac
     2401 cccgtccaat cgccagcatc tgctgctcgc cgcctgaaag atagccagca tgacttttgc
     2461 ggcgctcacg cagcaccgga aacaggtcca taactcgatc atatgcctgc tgcgtaccgg
     2521 caggattgcg cg
//
LOCUS       DASZAK010001042         1696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_239376, whole genome shotgun sequence.
ACCESSION   DASZAK010001042 DASZAK010000000
VERSION     DASZAK010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239376"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..620
                     /locus_tag="VGT44_18400"
     CDS             180..620
                     /locus_tag="VGT44_18400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582835.1"
                     /translation="MQGTQNQQNNPAAPDIAQLRRRAWRINGRSTLYLLVVCAPLAWA
                     ALLFFTFFVQPQSILAFAVFFLLLDLALTCMLVPLAYFIGLRFISSRLYRATMRHALR
                     QGVLLSLLIVFNLLLRALRSWSIFTALVSLAIVAVIEVLALARK"
     gene            635..1555
                     /locus_tag="VGT44_18405"
     CDS             635..1555
                     /locus_tag="VGT44_18405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV2582836.1"
                     /translation="MNIVIIRPGAIGDTLLTFPVLRALRAKYPDAHVTFVSNATVLPL
                     AQEAGLADEVFDFQLVQWSELFSTAGIRTPSLRALLHESDLAIGWMGDPDHLVARNLR
                     EAGVGQVMIAQGRPPQGERVHVTEYLARTIGIEALPGVLDLRGDEKPHRNAGATSIAL
                     HPGSGGKQKCWPVERFAEVIERVWQQRWPVLLLFGPADGKQLAYLRQRLVPPAPELLT
                     MLVDAPLIEVARQLRDCQCYLGNDSGITHLAAMLGLPTIALFGPSDPAVWHPVGPDSG
                     LVRVVHEPELAKLPVETVLELIMKFHNQDR"
BASE COUNT          312 a          487 c          531 g          366 t
ORIGIN      
        1 gtgatctgcc gcgttcgccc gcgcatcacg ccgcacggcg gggaaagcaa ggacgcgccc
       61 gacatctggc agaagcattt cagcccggag gccgaggaga gtcgccgccg ccacgaagcg
      121 cccttgaatg gagatcgcag ctttaagcta ctgaaatgaa cacacggaat tcacagcact
      181 tgcagggaac gcaaaaccag cagaataacc cggctgcacc ggatatcgct cagctccgtc
      241 gccgcgcctg gcgcatcaat ggccgctcca cgctttatct gctagtcgtg tgcgcgccgc
      301 tggcgtgggc cgcgctgctg ttcttcacat tttttgtgca accgcagagc atactggcct
      361 tcgccgtctt cttcctgctg cttgacctgg cgctcacctg tatgcttgtc ccgttggcgt
      421 acttcatcgg cctgcgcttc atctcctcgc ggctctatcg cgctaccatg cgtcacgccc
      481 tgcgccaggg tgtactgctc tccctgctga tcgtcttcaa cctgctgctg cgcgccctgc
      541 gcagttggag catcttcacc gccctcgtca gcctggcgat tgtggcggtg atcgaggtgc
      601 tggcgctagc gcgcaagtga atagcaggca aacgatgaat atagtcatca tccgtcccgg
      661 cgcaatcggg gacacgcttc ttacatttcc cgttttgcgg gcattgcgcg cgaaatatcc
      721 tgatgcacat gtcacattcg ttagcaatgc tactgttctg ccgctcgcgc aggaagctgg
      781 ccttgctgac gaggtttttg atttccaact ggtgcaatgg agcgaactgt tctccactgc
      841 tggcatacgg acaccttcac tgcgcgcttt actgcatgag agtgacctgg cgataggctg
      901 gatgggcgac cccgatcacc tggtcgcgcg gaatctgcgc gaagcgggcg tagggcaggt
      961 catgatcgcg caggggcgac caccgcaggg ggagcgcgtc cacgtcactg agtacctggc
     1021 gcgaacgata gggattgaag ctctgccagg tgtgttagat ttacgtggag atgaaaagcc
     1081 gcataggaat gcaggagcaa cgagtatcgc acttcatcct ggcagcggtg gcaagcagaa
     1141 atgctggccg gttgagcgat tcgctgaggt gatcgagcgg gtatggcaac agagatggcc
     1201 ggtgttgctg ttgtttgggc cagccgatgg caagcagctc gcctacctcc ggcagcggct
     1261 tgtcccgcct gcgccagagt tgctcacgat gctggtggat gcgccgttga ttgaggtggc
     1321 gcggcaattg agggattgcc aatgctacct gggcaacgac tccggcatta cacacctggc
     1381 ggcaatgttg ggtctgccga ccatcgcgtt attcgggccg agcgacccgg cggtctggca
     1441 cccggtcgga cctgatagcg ggctggtgag ggttgttcat gagccggagc tggcgaaact
     1501 gccggttgaa actgttttgg agctgataat gaagtttcac aaccaggatc ggtaagaagt
     1561 tgtcatcctg agcgcagcga aggatctcgg tccctggcgg gtgagatcct tcgctgcgct
     1621 caggatgaca ctcggcggca ctttctttcg gtgatcgccc cgccgggggg attgggcgcg
     1681 atgtagtgct ggaagc
//
LOCUS       DASZAK010001043         2354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_595791, whole genome shotgun sequence.
ACCESSION   DASZAK010001043 DASZAK010000000
VERSION     DASZAK010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_595791"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VGT44_18410"
     CDS             complement(<1..338)
                     /locus_tag="VGT44_18410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582837.1"
                     /translation="MEAILFYASALLVYFFIYNILTWGLNIQFGYTGILNFAFIVFMA
                     IGAYIYGVTSLTPPDQIAGQTYVLGLSWPFPLNLLAGGVVAALFGVLVGFVALKRLRS
                     DYMAIVTVAVG"
     gene            complement(343..1218)
                     /locus_tag="VGT44_18415"
     CDS             complement(343..1218)
                     /locus_tag="VGT44_18415"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2582838.1"
                     /translation="MLIYLHAIGFGLVTASILALASVGLSLQFGVTNYINFAYGELLT
                     FGAYFAWIANNQYGWNIWVALLVGAIMVGIVAVVINVLILQQFIRRQYPTLFMLVVTF
                     GLSLILANVILAIWGGDFRRYNVAPDTQVNIGPFLFTGQQLIIMGIAVVLMLALHVLL
                     TYTKLGKAMRAMSDNATLARNCGINTDLVTNFTWLLSGFMGGIAGIVLALNITSFQPA
                     FGGDFLFVIFAAVILGGIGRPYGAMAGALVIGIVTEVAAVPLNSSFKNDAAFAILILV
                     LLLRPQGIFSSEGKA"
     gene            complement(1390..2283)
                     /locus_tag="VGT44_18420"
     CDS             complement(1390..2283)
                     /locus_tag="VGT44_18420"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEV2582839.1"
                     /translation="MLVLRSLLFAPANHARHVEKALSGAADGAILDLEDAVAIDEKPA
                     ARLAAQRVLEQRASPPTFTKAYVRINALTTPFAYQDMLEVVRPGLDGIILPKTESAEQ
                     VATLDWLLSQLEGERGMPVGGVDLMPIIETASGLLHAQEIAGASPRVRRLNFGAGDFS
                     LDTNMTWTSGNEGILWARVQLVVASRAARLEPPLDTVFPNLDDLEGLATEAEQAKRLG
                     FQGKACIHPKQVEVVNATFTPGAEEMRQARLLIDAFEQAEAAGIASLRVGAQFVDYPV
                     AERARRVVELGEAVERRKTSR"
BASE COUNT          561 a          702 c          646 g          445 t
ORIGIN      
        1 cccacggcca ccgtgacgat tgccatgtaa tcgctgcgca gccgcttcaa ggcaacgaag
       61 ccaaccagca ctccaaacag ggccgctacc actccaccgg ccagcagatt gagcgggaag
      121 ggccaggata agcctagcac gtaagtctgc ccggcaattt gatcaggtgg cgtgagactg
      181 gtcacgccat agatgtacgc gccaatcgcc atgaagacga taaaggcgaa gttaaggatg
      241 ccggtatacc cgaactggat attcaatccc caggtcagaa tgttgtagat aaagaagtac
      301 accagcagtg ccgaggcgta aaacaagatc gcttccatcg ggttatgcct ttccttccga
      361 agaaaaaatc ccctggggcc gcagtaacag cacgagtatg aggatagcaa aagcggcatc
      421 gttcttaaag gacgaattca gagggacagc ggcgacctcc gtgacgatgc cgatgaccag
      481 cgccccggcc atcgctccat acggacgccc gataccgccc agaatgaccg ccgcgaaaat
      541 gacgaagagg aaatccccgc caaacgctgg ctggaacgag gtaatgttca gcgccagcac
      601 tatcccggcg atgccgccca tgaaaccact cagcagccag gtaaaattgg tgaccaggtc
      661 ggtattgatg ccgcagttgc gcgcgagtgt ggcgttgtcc gacatggcgc gcatggcctt
      721 ccccagcttc gtataggtca ataggacgtg gagcgcgagc atcaggacaa cggctatgcc
      781 catgatgatc agttgctgcc ctgtgaagag gaaggggccg atgttgactt gcgtgtcagg
      841 agcaacgtta tagcgtctga agtcgccgcc ccagatagct aatatcacgt tcgccaatat
      901 aagcgacagg ccaaaggtga ccaccagcat aaagagtgtc ggatactggc ggcgaataaa
      961 ctgttggaga atcagtacgt tgatcactac cgcgacgatg ccgaccatga tagcgccgac
     1021 gagcagcgca acccagatat tccacccata ctgattgttg gcaatccagg cgaaatatgc
     1081 cccaaaggtc agcaattcac cataggcgaa attgatgtaa ttcgtcacgc cgaactggag
     1141 cgacaagcct actgatgcca gcgccagtat tgaggcagtg accagtccaa atccaatagc
     1201 gtgcaggtaa ataagcatgg gctaccgtct tccctttctc tctgcttcga gtacaaccag
     1261 gcggcctaac ctcttagcac agctctatac cgaaaatgta atcgataggg cgtaaatagc
     1321 gcataaacgt cgtctacctc gctataaata gcgcgtaaat attcgtcgtc cgcgcccaaa
     1381 caaccagcat taccggctcg ttttcctcct ttctaccgct tctcccagtt cgacgacgcg
     1441 gcgcgcccgc tcggctactg gataatccac aaactgcgcg ccaacgcgca gcgaggcgat
     1501 gcccgccgcc tctgcctgct cgaaggcgtc gatgaggagt cgcgcctgcc gcatttcctc
     1561 cgcgccgggg gtaaacgtgg cattcaccac ctcaacttgt ttggggtgaa tgcacgcctt
     1621 gccctgaaag cccaaccgct ttgcctgttc tgcctcagtc gccagccctt ccaggtcgtc
     1681 cagattggga aagacggtat cgagcggagg ttccaggcga gccgcacgcg aggcaacaac
     1741 cagttgaacg cgcgcccaca ggataccctc attgccggat gtccaggtca tgttcgtatc
     1801 caggctaaag tcgccggccc caaaattgag ccgccgcacg cgcggcgaag ctcccgcgat
     1861 ttcctgggca tggagcaggc cgctcgccgt ctcaataatc ggcatcaggt ccactccgcc
     1921 gacaggcatc ccgcgctcgc cttctagctg gctcaagagc cagtcgagcg tggcgacctg
     1981 ttccgccgat tcggtcttgg gcagaatgat gccatccagg ccggggcgga ccacttccag
     2041 catatcctgg taggcaaacg gcgtagtcag cgcattgatg cgcacatacg ctttcgtgaa
     2101 ggtaggagga gaggcgcgct gttcaagaac gcgctgagcc gctagccgtg cggcgggttt
     2161 ctcgtcgatg gctaccgcgt cttccaggtc aagaatcgct ccatccgccg cgccgctcag
     2221 cgccttctca acgtggcgcg cgtgattggc cggggcaaag aggagactac gtagtacaag
     2281 catgattttc tccaacatcg gcatcggacg cgatcatcgc acccttacta atctgtcaag
     2341 gttgaagtgg cgtg
//
LOCUS       DASZAK010001044         3444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_667340, whole genome shotgun sequence.
ACCESSION   DASZAK010001044 DASZAK010000000
VERSION     DASZAK010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667340"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="VGT44_18425"
     CDS             <1..259
                     /locus_tag="VGT44_18425"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEV2582840.1"
                     /translation="GDLAAGGIGYMLGSRSAQQQAPQYQAPQYQPPVPQYQPNAAYQP
                     APAAQANNERLAQLKLLGHLRDSNLLTNDEFEEEKQKILHG"
     gene            complement(282..1040)
                     /gene="phoU"
                     /locus_tag="VGT44_18430"
     CDS             complement(282..1040)
                     /gene="phoU"
                     /locus_tag="VGT44_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV2582841.1"
                     /translation="MARVLLDKELQELDGQIQRLGALVDEALEKALEALGSGDIAKSG
                     MVIEGDTIIDSLRMAVEEHTIRLLTLQQPLGGRDLRFLTSALSIASDLERTGDGAAGI
                     AQIILRMAPLRGNTVQDEAKAQEGMNGADQVSEASILHSMLELGKDARRVLQGTMDAY
                     ARRDAKAARYIWEEDDVVDVRYHLVRHDLMAQLEGAQAIPALQSDSRVLQRATYLLWI
                     AHKLERVGDHCSNICERVVFIVEGETSIKHSQEV"
     gene            1291..2127
                     /locus_tag="VGT44_18435"
     CDS             1291..2127
                     /locus_tag="VGT44_18435"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV2582842.1"
                     /translation="MSTKRLLGVYAHPDDEGGVGGTILHYNTLGIESGLAYSTRGEVG
                     EISDPALATPENLGDVREGEMRAAAEVLGVKHLWFLGYRDSGMAGTPENDDPCAFAQT
                     SAADAVGKLVAIIRQFRPQVLVTFDETGGYGHPDHIAIYRHTTSAFYAAADAVQYPEH
                     GPAHMVSKLYYSSFPRSFIRQIGEFMQNEGYNGSFSNLDPNKLGLPDELISVRMNVEP
                     WIETKNRSWGMHRTQMNPDNPMARMPQDIMRKWRAYEYFQLAATRVGPDIEGENNLFA
                     RT"
     gene            2150..2473
                     /locus_tag="VGT44_18440"
     CDS             2150..2473
                     /locus_tag="VGT44_18440"
                     /inference="COORDINATES: protein motif:HMM:NF041216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CU044_2847 family protein"
                     /protein_id="HEV2582843.1"
                     /translation="MSEVENIKAELPNGSTLHIQTVMLGGEEPVADLGLPSFKNVTDA
                     IEGIATSVVDTLKKVKPNSATVEFGVEIGMESGQLTALLVKGTGTATLKITLQWGEIS
                     AGSNS"
     gene            2491..>3444
                     /locus_tag="VGT44_18445"
     CDS             2491..>3444
                     /locus_tag="VGT44_18445"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV2582844.1"
                     /translation="MAQSIYELVTNCTVRINVGNSKGTGFFVAPGTILTCAHVITPAQ
                     TSGIPIQIAWQGQSYTGTFQNDWVAIGPDLALLKVDISHHPCVLLAGSADPFSDLFSY
                     GFPDTEPTGTPTTFKCVGWLGPQYEFVKFKEGQVRPGMSGSPILNNETGSVCGIVQIT
                     LDRTSAAGGKGLLTKVIYQTFPALEEAQKQFHTGDKHWSDALTQSQRERLSLTWLPAP
                     TITGKIQVFFSYSHKDDDLKDDLDTALVMMRREGLIENWYDRDIEAGTAIDEEIEKHL
                     NDAHIILLLVSPAFLRSDYCYQTEMTSAMKRHETKTARVVPI"
BASE COUNT          848 a         1021 c          855 g          720 t
ORIGIN      
        1 cggcgacctg gcagctggag ggataggcta tatgctagga agcagatctg ctcaacagca
       61 agcgccgcag tatcaagcgc cgcaatacca acctccagtt ccgcaatacc agccgaatgc
      121 tgcttatcag ccagcgccag cagcccaggc aaacaatgaa aggcttgcgc agctcaagtt
      181 actcggacat ttacgagact ctaaccttct cacaaacgac gaatttgagg aagaaaaaca
      241 gaaaatcttg cacgggtaag cgcgtcgtcg cgcttgatcg gttacacttc ttgcgagtgc
      301 ttgatgctcg tttcgccttc gacgataaac accacgcgct cgcaaatgtt ggagcagtgg
      361 tcgccgacgc gctccagctt atgcgcgatc cagagcaggt aggtcgcgcg ctgtaagacg
      421 cgcgaatcac tttgcagcgc gggaatagcc tgcgcgccct ccaactgcgc catcaggtca
      481 tgacgcacca ggtggtagcg cacatcaacc acgtcgtcct cctcccagat atagcgcgcc
      541 gccttcgcat cccgccgcgc gtaggcatcc atcgttccct gcaaaacacg ccgcgcatcc
      601 ttgcccagtt ccagcatact atgcaggatc gatgcctcgc tgacctggtc tgcgccattc
      661 atgccctctt gcgcctttgc ctcgtcctgg acggtgttcc cgcgcagagg cgccatacgc
      721 aggataatct gcgcgatgcc agccgcgcca tcgcctgtgc gctccaggtc actcgcaatc
      781 gaaagcgcag aggtgaggaa gcgcaggtcg cgcccgccca agggctgctg caaggtcagc
      841 aggcgaatgg tatgctcttc gacagccata cgcaaactat cgataatcgt atcaccttcg
      901 ataaccatgc ccgatttcgc gatatcccca ctccccagcg cctccagtgc cttctccaac
      961 gcttcatcaa ccagcgctcc caatcgctga atctgcccgt ccagctcctg caattcttta
     1021 tctaaaagta cacgtgccat aatagcctgc tcccttctgc ggtcttatcc tattatatcg
     1081 tataatttct caacccggag ttcgctcccg ctaatacttt agcatatttc ttgcagaaaa
     1141 gatagagtat atttgtaaaa cgttcttcta ttcttaaagg ataagtgtaa tcctgctctt
     1201 atttgacagc gcatgagcta tgatgttaca gtacatctac agcatcgctg ctcatccttt
     1261 aagaccaatc aagaaagtac aggatcattc atgtcaacga aacgactgct aggagtctat
     1321 gcccatcccg acgatgaagg tggggtcggc ggtacgatat tgcattataa cacgctgggt
     1381 atcgagtctg gcctcgctta ctctacacgc ggcgaagtag gtgaaatctc cgacccggcg
     1441 ctggctacgc cggagaacct cggagatgta cgcgagggcg agatgcgcgc ggcagccgaa
     1501 gtgctgggcg tcaagcatct ctggttcctg ggctaccgcg actccggtat ggccggtacg
     1561 ccggagaacg acgacccgtg cgcctttgcc cagaccagcg ccgccgatgc cgtcggcaaa
     1621 ctggtcgcca tcattcgcca gttccgcccg caggtgctgg tcacattcga cgagaccggc
     1681 ggctacggac atcccgacca catcgccatc taccggcaca ccaccagcgc cttctatgcc
     1741 gccgccgacg ccgtacaata tcccgaacac ggcccggccc acatggtttc caagctctat
     1801 tattccagct ttccgcgcag tttcatccgt caaatcggcg aattcatgca gaacgagggc
     1861 tacaacggtt cattcagcaa cctggacccc aacaagcttg gcctgcccga cgaactcatc
     1921 agcgtgcgca tgaacgtcga accctggatc gaaaccaaaa accgctcgtg gggtatgcat
     1981 cgcacgcaga tgaaccccga caaccccatg gccaggatgc cccaggacat tatgcgcaaa
     2041 tggcgcgcct acgaatactt ccagctcgcc gccacgcgcg tcggccccga tatcgaagga
     2101 gaaaacaacc tgttcgcgag aacgtaaagg aggcaagcgg agggaatgca tgtccgaagt
     2161 agaaaacatt aaggcagagc tgccgaatgg ctcgacccta catattcaaa ccgtcatgct
     2221 cggtggcgaa gagcctgtgg ccgatttagg tcttccatca ttcaaaaatg taaccgatgc
     2281 catcgagggc attgctacct cggtagttga cacactcaaa aaggtgaaac caaacagtgc
     2341 caccgtcgag tttggagtgg aaattggcat ggagtcgggc caactgactg ctctccttgt
     2401 caaaggaacg ggaacggcaa ccttgaaaat tactctgcaa tggggcgaaa ttagcgccgg
     2461 ctctaattcg tagaggagga aaaaacgctc atggctcaat cgatctacga gcttgtcaca
     2521 aattgcacgg tgcgcatcaa tgttggcaat tcaaaaggaa ccggcttctt tgttgcgccc
     2581 ggtacgatac ttacctgcgc gcacgtcatc acacccgcgc aaacttccgg gataccgatc
     2641 cagattgcct ggcagggaca atcctatacg ggtaccttcc aaaacgattg ggttgccatt
     2701 ggtcctgatc tcgccttgct taaagtagat atatctcatc atccctgcgt gctgctggct
     2761 ggtagcgcag atccatttag cgatctgttc agttacggat ttcccgatac agaaccgacc
     2821 ggcacaccaa ccaccttcaa gtgcgttggc tggctcggcc cgcaatatga attcgtcaaa
     2881 ttcaaagaag gccaggtgcg acccggcatg agtggttcgc ccatcctgaa taatgaaacc
     2941 ggcagcgtct gtggcatcgt ccagattacc ctcgatcgta ccagcgcggc gggcggcaaa
     3001 gggttactta caaaagttat ctatcaaact ttcccggcgc ttgaagaggc acagaagcag
     3061 ttccatacag gagataaaca ttggtccgac gcgcttacac aatcacagcg cgagaggctg
     3121 agcctgacgt ggttgcccgc gccaaccatc accggcaaga ttcaggtgtt tttctcctat
     3181 tcgcataaag atgatgacct caaagatgac cttgataccg ccctggtcat gatgcgaaga
     3241 gaaggtttga tcgaaaactg gtatgatcgg gatatcgaag ctggtacagc gatagacgaa
     3301 gaaatcgaaa aacatctgaa cgatgctcac atcatactcc tcctggtcag ccccgccttc
     3361 ctacgctcag attactgtta ccagacagag atgacgagcg ctatgaaacg gcatgaaacg
     3421 aaaaccgccc gcgtcgttcc gatt
//
LOCUS       DASZAK010001045         5106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_202655, whole genome shotgun sequence.
ACCESSION   DASZAK010001045 DASZAK010000000
VERSION     DASZAK010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202655"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..3164
                     /locus_tag="VGT44_18450"
     CDS             171..3164
                     /locus_tag="VGT44_18450"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV2582845.1"
                     /translation="MSRDPNTAHPRMIGWIGTTALAMGGSNQSLFLIGALIVSQGSAA
                     VPLLMLGLLLSWAAAFGWTELVLMWPNRVGGIAATCAEAFRPYSPILANLTGVCYWWG
                     WVPTCGLTAILSATAITEWYLPRVPVPLLASLLVLGFMFVNLCGVRWVTRLAIPLASL
                     SAALAFLSGVIPVVAGQVDWRQAFTFHLINPFPGFFGGLTSAMAGLYLVGFAAPAFEA
                     ATCHVGETIRPERNVPRAVFASGFMATIYFAFLPIIWLGVLGPAPLTGDLAQTLGPTY
                     APWLGALAHAAAIWFMMFNMFHGTLQPLAGAARTLAQLAEDGLLPRVLARRSRTDAPW
                     VATLLTAGVAIAFLLTGDPTWIIAAANLTYLIGICLPNVAVWLLRRDEPDMPRPYRAP
                     RGTIVLGLMASVVWGIATLLGFEQFGLPTVIAGLVLAYSGSILYALRRWDDRRREGKR
                     LSFRSLHLKLTGAMLLVLALDGSGYVLAISCISSKDMALITGLEDIFVAVAILTISVG
                     LVLPGMIGHAVGEVARVADQLATGTLADLADAMQALQMGNLEKAEVRVDITPIVVRSK
                     DEVGGMAASFNTMQAQLGLVAVSLYGAREGLSLAYAKLKTSNTELQVAHDELATLAIT
                     DHLTGLPNHRALVATLAHELERATRYSRPCSILFVDLDHFKALNDSCGHAAGDAVLRE
                     LGEVIRESLRNIDTVGRWGGEEFLAVLPEADEASALAAAERVRVAVAGHVFGVGGGTY
                     LTCSLGIAVFPLDATDRSHLVEAADRAMYAAKKLGRNQVRTASEPAVLLVDGGSRAVG
                     SREDVALTGAVEALAALVEARDQYTGDHTSEVATLATQIALRLGLSEADARMVGLAGH
                     LHDLGKVAIPDDVLQKPSGLTPEEWELMRTHPVVGADVVSRMPSLRVLVPAIRGHHER
                     WDGEGYPDRLSGEDIALGARIIAVADAYGAMTTDRPYRKAGSPSWAMRELRRCAGVQF
                     DPTIVDALEQVLSTTMPLALYEEAV"
     gene            complement(3197..>5106)
                     /gene="ptsP"
                     /locus_tag="VGT44_18455"
     CDS             complement(3197..>5106)
                     /gene="ptsP"
                     /locus_tag="VGT44_18455"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV2582846.1"
                     /translation="PAALTALSELVQANFFETAAPASSAPTTPVTPITPAAPVVQPGR
                     AEHTWRGIPTSPGVALGPAYLFTSGAPSLHAIERLGIAPQQTAAEQERLKQAMLNAAQ
                     ELQHLATDLQSQIGQEQAAIFEAQALMLRDPSLLQTALNMIDEQRIDAASALAVVGEQ
                     QAAALEALDDALLAARAIDVRDALGRAVRLLRGKEEPKRAAFDRPVILLAQELTPSDT
                     AQLSLANVLGICTTRGGPTSHSAILARALGIPAIAGLDEAALHLIRPGDEIGLDAGSA
                     AQVYHLPSPETRQQLMQRVEAQRQERAARISAAQQGHAPLVIGARRILTMANIGSAAE
                     AEAARQWGAEGVGLLRTEFLFASAATLPGEEEQRQQYVKVFRAFIGATPRQAGPFVAR
                     TLDAGADKPMPALERALGATVEANPALGVRGVRIHLAHEELLEQQLGGLLRASAGTGI
                     ELHIMFPMITTVEELTAARAVFDRVYTRLQAQGLPLPTRVPVGIMVEVPAAALMAPEL
                     ADLADFFSIGANDLMQYTLAVDRTNAALTSLYNLMQPSTLRLIAQVAQAGRRAGKPVA
                     VCGEIASDARLAPLLVALGVDELSMTPTALADVRSELARWSAEQLDSLSKRFMRAKTV
                     AEVEQIFRPTE"
BASE COUNT          822 a         1497 c         1750 g         1037 t
ORIGIN      
        1 cacaacaccg taatcggtgg gtgtaggaac tagtttctac acccccttgc ctttttatac
       61 tttcttaata aaggggggtg aagagccgaa taaagactca tgacacatat tcgggatgag
      121 cttgtgctgg tggtagggag acaggcgaca gaaaggtgac accaggcgtc atgagtcgag
      181 accccaatac cgcgcatcca cgcatgatcg gctggatagg aacgactgcg ctcgctatgg
      241 gagggagtaa tcagagtctt ttcttgatcg gggcattgat tgtgagtcag ggcagcgcgg
      301 cggtgccgct cttaatgctt ggcttactgc tgagctgggc tgcggcattt gggtggacgg
      361 aactggtctt gatgtggccc aatcgggttg gcgggatcgc ggctacctgc gcggaagcct
      421 tccgccctta cagtccaata ctggcgaacc tgaccggcgt gtgctactgg tgggggtggg
      481 tgccgacgtg cggcctgacc gctattctct cggcaacggc tatcacggaa tggtatttac
      541 ccagagtgcc tgtgccattg ctggcgtcgc tgctggtgct ggggtttatg ttcgtcaacc
      601 tgtgcggagt gcggtgggtg acgcgcctgg cgatccctct tgcgtctctg tcggctgcgc
      661 tggcctttct ttcgggggtg ataccggttg ttgcaggcca ggttgactgg cgccaggctt
      721 tcacctttca tctgatcaat ccgtttccag ggtttttcgg cggcctgacc agcgccatgg
      781 ccggactcta cctggttggt ttcgccgcgc ccgcctttga agcggcaacc tgccacgtag
      841 gcgagacgat tcgcccggag cgcaatgtgc cgcgcgcagt ctttgccagc ggcttcatgg
      901 cgacgattta ttttgctttt ttaccgatca tctggctggg tgtgcttggc ccggctccgc
      961 ttaccggcga cctggcacaa accttaggac cgacgtatgc cccctggttg ggcgcgctgg
     1021 ctcacgcggc tgccatctgg ttcatgatgt tcaatatgtt ccacggcacg ttgcagccac
     1081 tggcaggcgc agcgcgcaca ctggcgcaat tggcggaaga cggtttgctg ccgcgcgtgc
     1141 tggcgcgccg ctcgcgcaca gacgcgccct gggttgcgac actgctgacg gcgggggtgg
     1201 caattgcctt cctgctgacc ggcgacccca cctggattat tgccgctgcc aacctgacct
     1261 acttgatcgg catctgcctg ccaaacgtgg cggtctggct gctgcgccgc gatgagccgg
     1321 acatgccgcg cccataccgt gccccacgag gcactatcgt cctggggctg atggcttccg
     1381 tcgtctgggg gatcgcgacg ctgctcggct ttgagcagtt cggtttgccc acggtgatcg
     1441 ctggacttgt cctggcgtat tccggctcga ttctctacgc gctgcgccgc tgggacgacc
     1501 ggcgcaggga aggcaaacgc ctgtcgttcc gctcgctcca tctcaagctg accggcgcga
     1561 tgctgctggt tttggcgctt gacggttcag gatacgtgct ggccattagc tgtatcagta
     1621 gcaaggatat ggctctcatc accggcctgg aagatatctt cgtggccgtc gctattctca
     1681 ccatttccgt tggcctggtg ctgcccggca tgatcggtca cgctgtaggg gaggtggcgc
     1741 gggtcgcgga ccaactcgct accgggacgc tggcagacct tgccgatgcg atgcaggcgc
     1801 tccagatggg caatctcgaa aaggcagagg tgcgcgtcga tatcacaccg attgtcgtgc
     1861 gctcaaaaga cgaagttggc ggcatggcgg ccagctttaa tacgatgcag gctcagcttg
     1921 ggctggtggc cgtatcgctg tatggcgcgc gcgaagggtt gagcctggcg tatgcaaagt
     1981 tgaaaacctc taataccgaa ttgcaagtcg cccacgacga actggcgacc ctggcgatta
     2041 ccgatcacct gaccgggctg ccgaatcacc gcgcgctggt cgcgacgctc gcgcatgaac
     2101 tggaacgggc gacgcggtac agccgaccat gcagcattct ctttgtggac cttgaccatt
     2161 ttaaggcgct gaacgatagc tgtggccatg ccgccggaga tgccgtgctg cgcgaacttg
     2221 gcgaggtcat ccgggaaagt ttgagaaaca tcgataccgt cggacgatgg ggaggtgaag
     2281 agtttctagc tgtgctgccg gaggccgacg aggcatcggc cctggcggcg gctgaacggg
     2341 tgcgtgtggc agtcgcgggg catgtcttcg gcgtcggggg cggcacgtat ctaacctgtt
     2401 cgctcggtat cgccgtcttc ccgctcgatg ctacagatcg ctcgcatctt gtcgaggctg
     2461 ctgatcgcgc catgtacgcg gcgaagaagt tgggccggaa ccaggtgcgc acggcgtcgg
     2521 aaccggcggt cctcctggtt gatgggggaa gtcgcgcggt cggctcacgt gaagatgtgg
     2581 cgctgacggg ggcagttgaa gcgctggcgg ccctggtgga ggcgcgcgat caatataccg
     2641 gcgatcatac aagcgaggta gcgacgctcg ctacgcaaat cgcgctgagg ctgggtctga
     2701 gcgaggctga cgcgcgcatg gtcgggctgg ccggccacct gcacgacctg gggaaggtgg
     2761 cgatccccga tgatgtcctg caaaagccgt ccggcctgac gccggaggaa tgggaattga
     2821 tgcgcacgca cccggtcgtc ggcgcggatg tggtgagccg tatgccgtcg ctgcgcgtgc
     2881 tggtgccggc tattcgcggg catcacgaac gctgggacgg tgaggggtat ccagatcgtt
     2941 tgagtggtga agatattgcg ctcggcgcgc gtatcatcgc tgtcgccgac gcatatggcg
     3001 ctatgacgac cgaccgtcct tatcgcaaag caggcagccc atcctgggct atgcgtgagt
     3061 tgcgccgctg cgccggtgtc caattcgacc caaccattgt agatgccctg gagcaggtac
     3121 tctccactac aatgcctctt gcattgtatg aggaagcggt gtaagtagga gacccttgcg
     3181 gtcgccctcc tcctctctac tccgttggcc gaaaaatctg ttctacttcc gcgacggttt
     3241 tggcgcgcat gaagcgcttt gacagggagt cgagctgctc ggcggaccag cgcgccagtt
     3301 cgcttctgac atcggcgagc gcggtggggg tcatgctcaa ttcgtccacg ccgagcgcga
     3361 cgagcagcgg agcgaggcgg gcgtcgctgg cgatctcgcc gcagacggcc accggcttgc
     3421 cggcgcgccg cccggcctgc gccacctggg cgatcagccg tagcgtggat ggctgcatga
     3481 ggttgtagag gctggtgagg gcggcgttgg tgcgatcaac ggccagggta tactgcatca
     3541 ggtcgttcgc gccgatactg aaaaagtcgg cgagatcggc gagttcgggc gccatcaggg
     3601 cggcagccgg gacctcgacc atgatgccca ccggcacgcg cgtgggtagg ggcagcccct
     3661 gcgcttgcag gcgcgtgtag acgcgatcaa agacggcgcg agcggcggtg agttcctcga
     3721 cagtggttat catggggaac atgatgtgca gctcaatgcc cgttccggcg gaggcgcgca
     3781 gcagaccgcc gagctgttgt tcgagcagtt cctcgtgcgc caggtgaatg cgcacgccgc
     3841 gtacccccag tgcaggattg gcctcgacgg tcgcgcccag cgcgcgttcc agcgctggca
     3901 tgggtttatc cgcgcccgcg tcgagcgtgc gcgccacgaa tggcccggcc tggcgcggtg
     3961 tagcgccgat gaaggcgcgg aacactttca cgtactgctg gcgctgctct tcttcccctg
     4021 gcagggttgc ggcggaggcg aacaggaact cggtgcgcag cagcccgacg ccttccgcgc
     4081 cccactgccg cgccgcctcg gcttctgccg cgctgccgat gttcgccatt gtaaggatgc
     4141 ggcgcgcgcc gatcacgagg ggcgcgtgtc cctgttgggc ggcgctgatg cgcgcggcgc
     4201 gctcctgccg ctgcgcctcg acgcgctgca tcaactgctg gcgcgtctct ggtgatggta
     4261 ggtgatagac ctgggcggcg ctcccggcat ccaggccgat ctcatcgccg ggacgaatga
     4321 gatgcagcgc ggcttcatcc aaccctgcga tggcggggat gccaagcgcg cgcgccagaa
     4381 tcgccgaatg cgacgttggc ccgccgcgcg tggtgcagat gccaagcaca ttagcaagac
     4441 tcagttgcgc tgtgtcggag ggtgttagtt cttgcgcgag caggatgacg gggcggtcga
     4501 acgcggcgcg tttcggctct tcctttcctc tgagcagacg gacggctctg ccgagggcgt
     4561 cgcgcacatc gatggcgcgc gccgccagga gtgcatcgtc gagcgcctcc agagcggcgg
     4621 cctgctgctc gccgacgaca gccagcgcgc tggccgcgtc gatgcgctgt tcatcgatca
     4681 tgtttaaggc ggtttgcagc agcgatggat cgcgcaacat cagcgcctgg gcctcgaaaa
     4741 tggcggcctg ctcctgcccg atctggcttt gcaggtcggt cgctagatgc tggagttcct
     4801 gcgcggcatt cagcatggcc tgtttcaggc gttcctgttc ggcggcggtt tgctgcggcg
     4861 cgataccaag gcgttcaatc gcgtggagcg atggcgcgcc ggatgtgaag agataggccg
     4921 gtcccagggc gacgccgggc gaggttggta tgccgcgcca tgtatgctca gctctgcctg
     4981 gctggacgac cggcgcggcg ggcgtgattg gcgtgacggg cgtggttggt gcagatgatg
     5041 caggggctgc ggtttcgaag aaattggcct gtaccagctc actcagggcg gtaagcgcgg
     5101 ctggcg
//
LOCUS       DASZAK010001046         5093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_525646, whole genome shotgun sequence.
ACCESSION   DASZAK010001046 DASZAK010000000
VERSION     DASZAK010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525646"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..996)
                     /locus_tag="VGT44_18460"
     CDS             complement(253..996)
                     /locus_tag="VGT44_18460"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV2582847.1"
                     /translation="MTQNTSVTPASPKTKTKPAKNLYEPYDTPMWFWRGLRAFARFIF
                     AIILDVHISGLEHIPKQGPCIVASNHLAWMDVPLVPAYMRRKVVYMAKEQLFYGKVGW
                     LVRLLGAFPVKRGEADRQSLRAADTQLKAGKIFIIFPEGTRSKNHQLAKGHVGLGMIA
                     LRSGVPVIPVAVWGSEKSLKTFRPRVTITYGEPMTLTPKGNKITREDIEEATDAVMSR
                     IASMLPEQYRGVYAVYGPSDAQEESEQSQ"
     gene            complement(993..1682)
                     /gene="cmk"
                     /locus_tag="VGT44_18465"
     CDS             complement(993..1682)
                     /gene="cmk"
                     /locus_tag="VGT44_18465"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV2582848.1"
                     /translation="MHRPGRIAIDGPAGAGKSTVGEQLARKLGYLYIDTGAMYRAVAW
                     LALREGVSVDDGPALARLAQRAEIAILHPHVDDGRQYTVTVDGQDVTWDIRNTAVTRA
                     VPPVSAHPDVRAILIARQREMAYRQDGIVMVGRDIGAVVLPDAELKIYLTATVQERAR
                     RRYAELEERLGKDNPALPSLDDVLADIERRDEIDKDHSAPAHDAIVIMSDNLSVEQVM
                     ELIESLVEDTP"
     gene            complement(1758..2015)
                     /locus_tag="VGT44_18470"
     CDS             complement(1758..2015)
                     /locus_tag="VGT44_18470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582849.1"
                     /translation="MQLIGILILIGVLALLLIGVFYLGSTQKTHTPPESSLDDFYRST
                     GFGNVVDANKRIREMGLSGGQRRHSRGKKPHRQQGKHEGEK"
     gene            complement(2006..2809)
                     /locus_tag="VGT44_18475"
     CDS             complement(2006..2809)
                     /locus_tag="VGT44_18475"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEV2582850.1"
                     /translation="MTRQEQGREEEQQGPQEQNEERLARYLAHAGVASRRHAEELIAA
                     GRVQVNGATVTTQGTRVVPGQDRVTVDSQLVQLPVSQVYILLHKPVGYVTTALDPQNR
                     PTVLDLLSEELRRLRVYPVGRLDIDSSGLLLLTNDGDFALRLSHPRYATQKHYEALVK
                     GLPTRDALNALRQGVEIKEDNGQLYHTSPAIVRVLRQAGATSWLSLTIHEGRKRQVRR
                     MLMAAGHPVLELRRVGIGALKLGDTPEGKWRYLTQDEVTTLLEGKKQCN"
     gene            complement(2806..3927)
                     /locus_tag="VGT44_18480"
     CDS             complement(2806..3927)
                     /locus_tag="VGT44_18480"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HEV2582851.1"
                     /translation="MLCIREVREIPQETDLAQRWHRLPLQTTLPLSSGARCRLVYAGR
                     PGGPQGPDIRDALVAFGHDVLCGNVEFHVRCSDWYTHRHHTDPHYNNVILHVVLIYDS
                     PAPVTRQDGQTVPVFSFNDIAPQRNPFPPATYPCERKMPEMAAERRDALLRQAGLERF
                     ELKTAHFLKLLRDAQPCEPFSAYDTCLIPSLAEALGSGRDRDFFRAAGLRLVGLLHDA
                     DLPEPQGRAPYPSPLDAGRLQALRKLIQQWRASGVWETIRHIVGNRQLIQALRAIFPG
                     ISSSRADIVICNVVLPFAVAVAIREDDMELYARARQAYLDYVGLASNQVTRAMSKQLR
                     LSREPDNACQQQGLHHIYTQTCREKRCGTCLVTQWRLEL"
     gene            complement(3936..4964)
                     /locus_tag="VGT44_18485"
     CDS             complement(3936..4964)
                     /locus_tag="VGT44_18485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582852.1"
                     /translation="MGRKQDTDAPRDERDESSKALRKREQKILVRLQEAQQAQAKAQA
                     RVERAQARLQKRTARVARIEARLLLIRQQLAEPEQPTPAEQLEVASEASLPEQSEATA
                     EEWQPAEARAVAEATEQRIRISAERATQVASQATPVSAPDRADIPDIPDIVDAPDTPV
                     EEDEQPEQPAQEEEPASIPIPPELLQIEEEEALLEASSAGTFAQIAAERATKTRVAAE
                     LSSEQTREARMQAQWAEQALGRVRLDIRYGVLSGEDADIALRQAEREVTRTQAALADA
                     EAAEEQALQAAINAEAAAEVAEGMAYATSDHLELLEYAGEGSEIAEDGEITLQMPVVH
                     PPSEESPA"
BASE COUNT          885 a         1557 c         1502 g         1149 t
ORIGIN      
        1 gtaaagtccc ttaaataacc atcccgctta ctaaagaaaa gtggcgtatc ctttcataag
       61 catagaataa cacagaaatg cctactttca aatgttacgg cctggcaagg aagccatcga
      121 gagttggatt gaggcaagcc acatctgggg cgatcctatt cctcctcctc gcaaactgac
      181 cacggcttca cgataggtgg gtttctgcac acgcagtggg tctgaggttc gagtcccatc
      241 gctctacatg agctactgcg attgttctga ttcctcctga gcatcagaag gtccatagac
      301 ggcatacacg cccctatatt gctcaggcag catcgacgcg atgcggctca ttacggcgtc
      361 ggtggcttcc tcgatgtctt cgcgcgtgat cttgttgcct ttgggggtta gcgtcatggg
      421 ttcgccgtag gtgatggtga cgcgcgggcg gaaggttttg agcgattttt cgctgcccca
      481 cacggcgacg gggatgacgg ggacgccgga gcgcagggcg atcatgccca ggccgacgtg
      541 tccctttgcc agctggtggt tcttgctgcg cgtcccctcc gggaagatga tgaagatttt
      601 tccggccttg agctgtgtat cggcggcgcg cagagattgc cggtcggcct cgccgcgctt
      661 gacggggaac gcgccgagca ggcgtaccag ccagcctact ttgccataga aaagctgttc
      721 ctttgccatg tagacgactt tgcgccgcat ataagccgga acaagcggca catccatcca
      781 cgcgagatga ttcgaggcga cgatgcaggg gccttgcttc gggatgtgtt ccaggccgga
      841 gatgtgtaca tccaggatga tcgcgaaaat gaaacgcgcg aaggcgcgca gcccacgcca
      901 gaaccacatc ggcgtatcgt aaggttcata caggtttttc gccggtttcg tcttcgtttt
      961 aggggacgcc ggggttacgc tggtattctg cgtcatggcg tgtcctccac gagactctca
     1021 atcagttcca tgacctgctc aacgctgagg ttgtcggaca taatcacaat ggcgtcgtgc
     1081 gcgggtgcgg aatggtcttt gtcgatctcg tcgcgcctct cgatgtcggc cagcacatca
     1141 tcaagagatg ggagcgcggg gttgtctttg cccaggcgtt cttccaactc ggcatagcgg
     1201 cggcgcgcgc gttcctgcac ggttgcggtc aggtagattt tcagttcagc gtctggcagc
     1261 acgaccgcgc cgatatcgcg cccaaccatc acgatgccgt cctgcctgta tgccatttca
     1321 cgctgccggg cgatcaagat ggcgcgcacg tccgggtgcg ccgaaacggg gggaacggcg
     1381 cgcgtgactg ccgtattgcg gatgtcccat gtgacatctt gcccatctac cgtgacggta
     1441 tactggcggc catcgtcgac atgcgggtgg aggatggcga tttcggcgcg ctgtgccaac
     1501 cttgccagcg cgggtccatc atcaacgctg accccttcgc gcagcgccag ccaggctact
     1561 gcccggtaca tcgcgcccgt atcgatgtat aaatacccaa gcttgcgcgc cagttgttcc
     1621 cctacggtgc ttttgccggc tcccgccggt ccgtcgatgg cgatgcgccc aggccgatgc
     1681 atgctccctc cccgtgcgtc ataaataggt ggcacataat ttagagggat tatatcacat
     1741 gctctcggcc tcttgtttta cttctcgccc tcatgtttcc cctgctggcg atgcggcttt
     1801 ttcccccgtg aatgccgtcg ttgccctccc gataaaccca tctcgcgaat gcgcttattc
     1861 gcatccacaa cgttcccaaa tccagttgag cggtaaaagt catcgagtga tgactccggt
     1921 ggcgtatgcg tcttttgggt actgcccaga tagaagacac caatgagcag gagagccaga
     1981 actcctatga ggatcagtat cccgatcagt tgcattgctt tttcccttcc agaagcgtcg
     2041 tcacttcatc ctgcgtcaaa tagcgccact tcccctcggg cgtatctccc agcttcaacg
     2101 ccccgatgcc cacacgtcgc agttccagca caggatgtcc cgctgccatg agcatgcgcc
     2161 gcacctgccg cttgcgccct tcgtgaatcg tcaacgagag ccagctagtt gctcctgctt
     2221 gccgcagaac gcgcacaatg gcaggggatg tatggtacaa ctgcccgtta tcttctttga
     2281 tctcgacacc ctggcgcagg gcgttgagcg cgtctcgcgt cggcaagcct ttgaccagcg
     2341 cctcgtaatg cttctgcgtg gcgtaacgcg ggtgggacag gcgcaatgcg aaatcgccgt
     2401 cgttggtcag gagcagcagg ccgctgctat cgatatcgag gcggccaacg ggatagacgc
     2461 gcaggcggcg cagttcctcc gacagcaaat cgagcacggt cggcctgttc tgtggatcga
     2521 gagccgtcgt cacatatccc accggcttat gcagcaagat gtatacctga ctgactggca
     2581 actgaacgag ttgactatcg acggtgacac gatcttgtcc aggaacgacg cgcgtcccct
     2641 gcgtcgtcac cgttgcgcca ttgacctgca cgcgcccggc tgcgatcaac tcctccgcgt
     2701 gacggcgtga ggcgacgccc gcgtgtgcca ggtagcgcgc caggcgttct tcattctgct
     2761 cttgcggacc ttgctgttct tcttcacgcc cctgttcctg ccgagtcata actcaagcct
     2821 ccattgcgtg acgagacatg tcccgcagcg tttctcccgg cacgtctgcg tgtagatatg
     2881 atgcagcccc tgctgctggc aagcgttgtc cggctcgcgc gagaggcgca actgtttgct
     2941 catcgcgcgt gtcacctggt tcgaggccag cccgacatag tccaggtacg cctgccgcgc
     3001 ccgcgcatac agttccatgt cgtcttcccg tattgccacc gccaccgcga agggcagcac
     3061 gacattgcag ataacaatat cagcgcgcga ggaactgatg ccagggaaga tagcccgaag
     3121 cgcctgaatc agttgccgat ttccaacgat atgtcggatc gtttcccaga cgccagaggc
     3181 gcgccactgt tggatgagtt tcctcagagc ttgcaggcgt cccgcatcga ggggcgaggg
     3241 atagggggcg cgtccctgcg gttcggggag gtcggcgtcg tgaagcagcc cgaccaggcg
     3301 cagaccggcg gcgcggaaaa agtcgcgatc tcgcccggag cccagcgcct cggccagcga
     3361 ggggatcagg caggtatcgt aggcgctgaa cggctcgcag ggttgcgcgt cacgcagcag
     3421 tttgaggaaa tgtgctgttt tcagctcgaa acgctccagt cccgcctgcc gcaaaagcgc
     3481 gtcgcggcgt tctgcggcca tttcaggcat cttgcgctcg cagggatagg tcgccggggg
     3541 gaaagggttt ctttgcggcg cgatgtcgtt gaacgaaaag acgggaacag tctgcccatc
     3601 ctgccgtgtg acgggcgcgg gcgagtcgta gatgagcacg acatgcagga tgacgttgtt
     3661 gtaatgtgga tcggtgtggt gccggtgcgt ataccagtcg ctacagcgca cgtgaaactc
     3721 gacgttgccg caaagcacat cgtgcccgaa agcgaccagc gcgtcgcgga tatcaggccc
     3781 ctgcggcccg cctgggcgac cggcatacac caggcgacag cgcgcgccgc tgctgagcgg
     3841 cagggtagtc tgcaatggca ggcgatgcca gcgctgcgcc aggtcggttt cttgtggtat
     3901 ttcccggact tctcgtatgc acaacataag gcatcctatg cgggtgactc ttccgatggt
     3961 ggatgcacga ccggcatctg caaagtaatc tcgccatcct ctgctatttc actaccctct
     4021 ccggcgtact ccagcagttc gaggtgatcg cttgtggcgt aggccatgcc ttcagctacc
     4081 tctgcggctg cttcggcatt gatggcggct tgcagggcct gctcctcggc tgcttcggca
     4141 tcggccaggg ctgcctgggt gcgcgtgacc tcgcgttccg cctgtcgcag ggcaatgtcg
     4201 gcatcttcgc cgctcaacac gccatagcgt atatccaggc gtactcgacc gagcgcctgc
     4261 tcagcccatt gcgcctgcat gcgcgcttcg cgtgtctgtt cgctgctgag ttctgcggca
     4321 accctggtct tcgttgcgcg ctctgccgcg atttgcgcaa atgtcccggc ggacgacgct
     4381 tccagtagcg cctcttcttc ctcgatctgg agcaactcag gcggaatcgg tatcgaagcg
     4441 ggttcttcct cttgcgcagg ttgctctggc tgctcgtctt cctcgacggg ggtatctggc
     4501 gcatccacta tatccggtat atctggtata tcggctctat caggcgcgct caccggcgtg
     4561 gcctgtgacg cgacctgtgt ggcgcgttca gccgagatgc ggatacgctg ttcggtcgct
     4621 tctgcgacgg cgcgcgcttc tgctggctgc cattcctcgg ctgttgcttc ggactgctca
     4681 ggaagcgatg cctcgcttgc aacctcaagt tgctcagcag gggtgggttg ctccggctcc
     4741 gccagctgtt gccggatgag caataagcgc gcttcgatac gcgccacgcg cgccgtgcgt
     4801 ttttgcagcc gcgcctgcgc tcgctcaacg cgcgcctgtg cttttgcctg agcctgctgc
     4861 gcctcctgca agcgtacaag tatcttttgc tcgcgtttgc gcagcgcctt gctcgattcg
     4921 tcccgctcat cgcgcggtgc atcggtgtct tgcttcctgc ccatggtggc tgccctccag
     4981 cgctatgctg ctactttttg cctttggcct tctttttcgt ccgcccattc aattttacat
     5041 cgtgcggcac tgctactttg taatcaaacg tatcgatagg ctgactctcc ttg
//
LOCUS       DASZAK010001047         3518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_202674, whole genome shotgun sequence.
ACCESSION   DASZAK010001047 DASZAK010000000
VERSION     DASZAK010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202674"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VGT44_18490"
     CDS             <1..324
                     /locus_tag="VGT44_18490"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEV2582853.1"
                     /translation="LGASGTARASVSQDKPVFGATHVYMRDEAFSPARIQVVLGTTVT
                     WTNQDTVPHSVRLSPAMMSSSDDWESGLLYPGQSFSYTFTSRGTFPYYCQEHPYEMGG
                     TVTVT"
     gene            complement(429..986)
                     /locus_tag="VGT44_18495"
     CDS             complement(429..986)
                     /locus_tag="VGT44_18495"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEV2582854.1"
                     /translation="MWRSEIRLAGAGGQGLGLAAVVLAESALACGYEASLMQAYGPEA
                     RGGASYADLVIADAPIANPRFEHPSMLLVLNQKAWNRFARFPPREDTITVIDALLVHP
                     ACIIPGLLAFPFERIAREELREKIAANMVAVGVLGHLLGRIPSEVLERTAALRSTEQF
                     RRTNREAVGRGWQLVASSPLSEVSL"
     gene            complement(973..1818)
                     /locus_tag="VGT44_18500"
     CDS             complement(973..1818)
                     /locus_tag="VGT44_18500"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2582855.1"
                     /translation="MISVKPLLRLDMMPHILCPGCGHGIVMGAMLRVLARRFTSLDQV
                     ALVAGIGCSSRIVGYIDACTLHTTHGRALTFATGLKLARSELTVIVVTGDGDGLAIGG
                     NHLIHAARRNIDLTCLLFNNEIYGMTGGQAAPTTVPHAYTTTTPYHNEELPFDAARLV
                     EAAGGTFVARAQAFRPDLLEEVLDQAIEHPGFAFIDVMTDCTEYFARKNSLGSGSDLL
                     FSQGRSEGEIAQDLSARGTLHPTDGWPIRATQPMGVLHRSRRPTLEEQLGLVLAKGDG
                     ANVAQ"
     gene            complement(1815..2972)
                     /locus_tag="VGT44_18505"
     CDS             complement(1815..2972)
                     /locus_tag="VGT44_18505"
                     /inference="COORDINATES: protein motif:HMM:NF006412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEV2582856.1"
                     /translation="MPAKRLVQGNEACAMGALAADCRFFAGYPISPSSEIAEYMARQL
                     PRVGGVFIQMEDEMASMGAVLGAALGGKKAMTATSGPGFSLMQENLGYASLAEIPCVV
                     VDVMRVGPSTGVPTAPAQGDVMQARWGSHGDRAAIALAPASVAEVYELTIASFNLAER
                     LRTPVILLYDEVIGHMREGVVLAEQAAADFWRRPFPDQEPGAGFQPYRPNDEGVAALP
                     DFGVGYRFHVTGLMHDERGYPTTDPVAVNRLLERLDKKVAGVEDWLAPERYRLEDADV
                     VFIAYGITARMARQAVDHLRERGMQAGLFRPRALWPFPAAAVQAATRQARAVVVAEMN
                     LGQWVYAVEHALAGAVPVVRCLKVGGDLITEHELEEQALPLFQGQQGVIVR"
     gene            complement(2962..>3518)
                     /locus_tag="VGT44_18510"
     CDS             complement(2962..>3518)
                     /locus_tag="VGT44_18510"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEV2582857.1"
                     /translation="PAALSDHLHGQFAYPLEEIDHPQLTVIDAFALAKEAGDGRAQGT
                     VLLGALSHRMGFPVETWREAIARWVPTKALAVNLQAFDLGRQWQPATLTCSPAQTRPP
                     ELRATYRVTIEPAWCKGCAICLDVCPERCFVLDEQERAVAVHQERCTGCQLCVKLCPD
                     FALEVNTVSLAAHVQEKGDTVNAS"
BASE COUNT          714 a         1136 c         1036 g          632 t
ORIGIN      
        1 ttgggagcga gcggcacggc gcgggcgtcg gtctcgcaag acaagccggt ctttggcgcc
       61 acgcatgtct acatgcgcga tgaggcgttc tctcccgcgc gtatccaggt ggtgctcggc
      121 acgacggtga cctggacgaa ccaggacacc gtccctcata gtgtgagact ctcccctgcc
      181 atgatgtcct cgtccgacga ctgggagagt gggctgctct acccggggca gtcgttcagc
      241 tacaccttca cgtcgcgcgg gacattcccg tactactgcc aggagcatcc ctacgagatg
      301 ggcgggacgg tgacggtgac gtagcaagcc ggcgaaagga tggtaggcag caagtttccc
      361 tacaagccca aacgctgaac accggtcaag taatcgagaa agggactggc agagggagaa
      421 aagcatggtc agagcgaaac ctcactgagt ggtgaactgg ccaccagctg ccacccacga
      481 cccaccgcct ccctatttgt gcggcggaac tgctcagtag aacgcaatgc ggcggtgcgt
      541 tccagcacct ccgacgggat acgtcccagc agatgcccca acacgcccac ggcgaccatg
      601 ttcgccgcga tcttctcacg cagttcttca cgcgcaatgc gttcaaaagg gaaagcaagc
      661 aagccgggga tgatgcatgc tggatggacc aggagcgcat cgatcacagt gatggtgtct
      721 tcccggggtg ggaagcgggc aaagcgattc catgctttct ggttgagcac caggagcatg
      781 gaaggatgct caaagcgtgg gttggcaata ggagcatcgg caatgacgag atcggcgtag
      841 ctggcgccgc cgcgcgcttc aggaccatat gcctgcatga gggatgcttc atagccacac
      901 gccagggcgc tctccgcgag caccacggca gccaggccga gtccctggcc gcccgccccg
      961 gccaggcgaa tctcactgcg ccacattggc accatctcct ttcgccagca ccaggccgag
     1021 ctgctcttca agcgtcggac gcctgctgcg gtgcaacacg cccatcggct gcgttgcgcg
     1081 aatcggccag ccgtctgtcg ggtgcagcgt accgcgcgcg ctcaagtcct gtgcgatctc
     1141 tccttcggac cggccctggc taaacagcag gtccgatccg ctccccaggg agtttttgcg
     1201 agcaaagtat tcggtgcagt cggtcatcac gtcgataaag gcaaagccag ggtgttcgat
     1261 tgcctggtcg aggacctctt ccagcagatc cgggcggaag gcttgcgccc gtgcgacgaa
     1321 cgtccccccg gcagcctcca ccaggcgagc ggcgtcgaat ggcagttcct cgttgtgata
     1381 gggagtggtc gtcgtataag catggggaac cgtcgtagga gcagcctggc cgcccgtcat
     1441 cccatagatt tcattgttga acaggagaca ggtgaggtcg atattgcggc gcgccgcgtg
     1501 aatcaggtgg ttgccgccaa tggccaggcc atcaccgtcg cctgtcacaa caatcacggt
     1561 cagttctgat cgcgccagct tcaggcccgt cgcaaacgta agcgcccgac cgtgggtggt
     1621 gtgtaaggtg caggcatcaa tgtagccaac gatgcggctg gaacagccga tgcctgctac
     1681 caacgccacc tggtcgaggc tcgtgaagcg ccgggccagc acgcgcagca tggcgcccat
     1741 cacaatgcca tgcccgcatc cgggacatag gatatgaggc atcatgtcga gccgtaaaag
     1801 cggctttact gatatcatcg tactatcact ccttgctgac cttgaaagag cggcagcgcc
     1861 tgctcctcaa gttcgtgctc ggtgatcagg tcgccaccga ctttaaggca gcgaacgacc
     1921 ggtaccgcac ctgctagcgc atgttccact gcatagaccc actggccgag gttcatctct
     1981 gctaccacca cggctcgcgc ctgccgcgtg gcagcctgca ctgccgcagc tggaaacggc
     2041 cagagcgccc gtggacgaaa cagcccggcc tgcatgcctc gctcgcgcag gtgatctacc
     2101 gcctgccgcg ccatacgggc agtgatgccg taggcgatga agaccacgtc agcatcttcc
     2161 agccggtaac gttccggggc aagccagtcc tcaactcctg ccaccttctt gtccagtcgt
     2221 tccagcagcc gattgactgc cacagggtcc gtcgttgggt agccacgctc atcgtgcatc
     2281 aacccggtca catggaagcg gtagccgaca ccgaagtcgg gcagcgcagc caccccttca
     2341 tcatttggcc ggtagggctg gaaacccgcc cctggctcct ggtcagggaa gggacgccgc
     2401 cagaaatcag cggcagcctg ctccgccaac accacccctt cccgcatatg accgatgacc
     2461 tcgtcgtaga gtaagattac cggtgtgcgc aggcgctcgg cgagattaaa actggcgatg
     2521 gtcagttcgt atacttcagc aaccgaagcc ggggccagcg cgatcgcagc gcgatctcca
     2581 tgtgatcccc agcgcgcctg cattacatcg ccctgggcag gggcggtcgg aacgccggtg
     2641 ctcggcccaa cgcgcatgac atccaccacc acgcagggga tctccgccag cgaggcataa
     2701 cccaggttct cctgcattaa ggaaaatccc ggtcccgaag tcgcggtcat cgcttttttg
     2761 ccaccgaggg cggctccaag cactgctccc atggaagcca tctcgtcctc catctggata
     2821 aacacgccgc caacgcgcgg taactgccgc gccatatact ccgcaatctc agaggaaggc
     2881 gagatgggat agccggcaaa aaagcggcag tcggcagcca gcgctcccat ggcacatgct
     2941 tcattcccct gtacaagccg tttagctggc attgaccgtg tccccctttt cctggacgtg
     3001 cgctgcaaga ctgaccgtat tcacttcaag tgcgaaatcc ggacacaact ttacgcacaa
     3061 ctggcagcct gtgcagcgct cctggtgaac ggccactgct ctctcctgct catccagcac
     3121 aaagcagcgc tcagggcaga catcaaggca aatcgcacag cctttgcacc aggcaggctc
     3181 aatcgtcacc cggtaggttg cccgcaactc cggcggcctg gtttgcgcag gcgaacaagt
     3241 gagcgtggcg ggttgccatt gccgccccag gtcaaaggct tgcaaattga ccgccagcgc
     3301 tttcgttggt acccaacgcg cgatggcttc gcgccacgtc tcgaccggaa accccatgcg
     3361 gtgcgagagc gcccccagca ggactgtccc ctgtgcgcgc ccatcgccgg cctcttttgc
     3421 cagcgcaaac gcatcaatca ctgtcagttg cgggtgatca atctcttcga gtgggtaggc
     3481 aaactgtcca tggaggtgat cactcagggc tgctggcg
//
LOCUS       DASZAK010001048         3489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_239460, whole genome shotgun sequence.
ACCESSION   DASZAK010001048 DASZAK010000000
VERSION     DASZAK010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239460"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VGT44_18515"
     CDS             <1..300
                     /locus_tag="VGT44_18515"
                     /inference="COORDINATES: protein motif:HMM:NF014848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA2 domain-containing protein"
                     /protein_id="HEV2582858.1"
                     /translation="SKRPVLPLSRSAAFVRQTMQRLTHERGYTSFLFMIVDIINMQCT
                     LLIIGGERAVAEVLKSPLQSGGHAIIVEGLVSRKKQLVPLLGRIQALLATERSPS"
     gene            complement(302..1429)
                     /locus_tag="VGT44_18520"
     CDS             complement(302..1429)
                     /locus_tag="VGT44_18520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582859.1"
                     /translation="MHVLCRFIAFCLLLMLCLTLSASQVHADGGAPNLAYVAGTSQGI
                     SVIDILQQKVTRTIAVDGDPYTILLSLDGRFLYVTQPATGRVTVIIARTGQTFCSVDL
                     PGHPTLLASGTDTTTLYAADNAGNIAIIDSTTCTVRKTIETHNQVNGMAVVGVISGNA
                     LTDQLWIAGSDALLLFNEQGQQLASVPIVGGPQSLCIPAGLTAYVTTRQGTVVAVDLG
                     TLQVSPPLLSGGTFGTMDYDAITLEVFVPDQEAKQIDVLSPFIVSEALPPQEPERVIR
                     LSGSPQAVAISSDGQFAFVALSRGEVEMLDLPARRAIKTITVGGTPHFIITGLYPPPG
                     TIAPLQAVASPLIPEVIVSALAVGALLVALWFAWRQRSKPG"
     gene            complement(1649..2647)
                     /locus_tag="VGT44_18525"
     CDS             complement(1649..2647)
                     /locus_tag="VGT44_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582860.1"
                     /translation="MLLSRKALQRVLGVLWLIDGLLQFQPQMFTMNMVNGIMKPMLSG
                     QPGLFEPSLQFIVTQTTLHLVEVNLLIGVVQVLLGLGFLLLPERWVKELVIASFVWAF
                     IVWYGGEGMSMLLTGTGSVLSGAPGAVLLYPLLGLAVYPRKRSKASSKEAAAWSRDNG
                     LLSRRVLRWVLAGFWFFAALLQLQPNWWQSGQISGAIGAMVGQGGLNRVLVDPVLTQI
                     SNATANIEVLLNIALIAVFLAIGIGLAVARDNQLRPFLIASILMSVVIWYCSEAFGMI
                     LTGMATDFNSGLLLVAMALAVGPYVRIKQAESARTRYAREMREDSQVVGSGSTQRA"
     gene            3309..>3489
                     /locus_tag="VGT44_18530"
     CDS             3309..>3489
                     /locus_tag="VGT44_18530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582861.1"
                     /translation="MAGTTQGISIINIARRRVTGTMAVAGKTHTVLLSPDGYALYVTQ
                     PTLGRVAAIAAKTGKT"
BASE COUNT          759 a         1021 c         1020 g          689 t
ORIGIN      
        1 tcgaaacggc cagtccttcc actatcgaga agcgccgcgt tcgtgcgcca gaccatgcag
       61 cggctgacgc acgaacgcgg ctacacgtcg tttctcttta tgattgttga tataataaat
      121 atgcaatgca ccctgctcat cattggagga gaacgcgccg ttgccgaggt gttgaaaagt
      181 ccgctccagt cgggaggaca cgcgattatc gtggaagggc tggtctcgcg caaaaaacaa
      241 ctcgtgccac ttctggggcg tatccaggcg ctgctcgcaa ctgagcgctc ccccagctag
      301 tttagccggg cttcgagcgt tgccgccacg cgaaccagag tgccacaagc agcgctccta
      361 cagcgagggc agagacgatg acttcgggga tcagcggtga cgctaccgct tgcagtggag
      421 cgatagtgcc cggtggtgga tagagaccag taatgatgaa gtgtggcgta ccgccgacgg
      481 taatcgtttt gatcgctcgc cgggcgggaa ggtcgagcat ctccacctcg cccctgctga
      541 gcgcaacgaa ggcgaattgc ccatcgctac tgatggcaac tgcttggggt gagccgctca
      601 gccggatcac gcgctccggt tcctgcggcg gaagggcttc acttacaata aatggggaga
      661 ggacatcgat ctgtttcgcc tcctgatcgg gaacaaagac ttccagggtg atcgcgtcat
      721 aatccatcgt gccgaatgta cccccactca gcaagggagg agagacctgc aacgtaccca
      781 ggtctaccgc gacaacggtc ccctggcgcg tcgtcacata ggcggtcagc cccgccggga
      841 tgcagagcga ttgcggaccc cccacaatcg gtacgctggc aagctgttgc ccttgctcgt
      901 tgaaaagcag gagcgcatcg ctcccggcga tccagagctg gtcggtcaat gcattaccgg
      961 agatgacgcc gacgaccgcc atgccattga cctggttgtg cgtctcaatg gtcttccgca
     1021 cagtgcaggt cgtggaatca atgatagcga tattccctgc attatctgcc gcatacagcg
     1081 tggtcgtatc tgtcccagac gccaggagcg tcggatgtcc gggcagatca acgctgcaaa
     1141 atgtctgacc ggttcgagca atgatgactg tcactcgtcc agttgccggt tgcgtcacat
     1201 acaagaaacg cccgtccagg ctcaataaga tcgtataggg gtcgccgtcc acagcgatcg
     1261 tgcgggtgac tttttgttgc aagatatcga taacggagat tccctgcgag gttccggcga
     1321 cgtaggccag gttcggcgcg cctccatcgg catgcacctg cgaggctgag agggtcaaac
     1381 agagcatgag cagcaggcag aacgcaatga atcggcataa gacatgcatt tctcaccttg
     1441 atcgagttgt gggatcacgt ggtgttttga ccagtgtagg ggatgtggtc gaaaacgtca
     1501 agtgtttttc tcaccccttc ttctttccat cagttcaatg aaaactgctt gttataccac
     1561 acctcctatt ggttgatgat cgtagtacac agacgcggct cggcatccgg tgcgcataca
     1621 ggctgcatgc gcaccggatg gccgttagtt aggcgcgttg ggtcgagcct gagcctacca
     1681 cctggctgtc ctcacgcatt tcacgggcat accgggtacg agccgactcc gcctgcttga
     1741 tgcgcacata aggcccgacc gccagcgcca tggccaccag caacaacccc gaattgaaat
     1801 cggtcgccat gccggtcagg atcatgccaa aggcttcgga acaataccag attaccacgc
     1861 tcatgaggat cgaggcgatc aggaaggggc gcagctggtt gtccctggcc accgccagac
     1921 cgatcccgat cgccaggaag acggcaatca gcgcgatgtt gagcagcact tcgatgttgg
     1981 ccgtggcgtt ggaaatctgc gttaagacgg ggtcgaccag cacacggttc agcccgccct
     2041 ggccgaccat cgcgccgatg gcccccgaga tctgtcccga ctgccaccag ttgggctgca
     2101 gctggagcag cgccgcgaag aaccagaagc ccgccaggac ccagcgcagc acccgccgcg
     2161 agagcaggcc gttgtcacgg ctccacgccg cggcctcctt cgaggaagcc ttcgagcgct
     2221 tgcgcgggta aaccgccagg cccagcagcg gatacagcag cactgccccc ggcgcacccg
     2281 acaggacgct gcccgtcccg gtgagcagca tgctcatacc ctcacctccg taccagacga
     2341 tgaaggccca cacgaaggag gcgatcacca gttctttgac ccagcgctca ggaagcaaga
     2401 ggaagccgag acccagcagg acctggacga cgccgatgag cagattgacc tcaacgaggt
     2461 gcagcgtcgt ctgggtgacg atgaactgca ggctcggctc aaaaaggccg ggttggccac
     2521 tcagcatggg cttcatgatg ccattgacca tgttcatggt gaacatctgc ggctgaaact
     2581 gtaagaggcc gtcaatcagc cacagcactc ccagtacccg ctgcaacgct ttgcgcgata
     2641 gtagcataat ggtatcgtat cctttcaaat cttctccgga agcaccgacg caggcgcccg
     2701 tgatcgtgcg tagcgtcact cgctcggggg aggtcgctca accgtaaagc tgttccggtt
     2761 ggaagcggat caccctatgt gactatagag cagtgcacct cagtcgtcta ttgggagaag
     2821 tccctatttt tctttggggg tctgatcgaa cagtcaaagc gttctcccct gcaaaacgcc
     2881 cttttcggtg ccatcgatcg ctccagggta tcagcaagaa atacgtgcgc agcgcttccc
     2941 agcctggggt gtaagcgaag aggagaaacg agccgctagt ggggcagttt gagtcagatt
     3001 agacaccccg cacgggtagg aggaatccct agtattcact ttagggtgca ggtcacctta
     3061 ggtcaggtgc aggcgcagaa ccgacaagcc cgtatccctc gacggctttg ctataatacg
     3121 agagacgacc ttacccgggc gatctgcgtc agtctatggc atggtggcac gaaacacaga
     3181 tggaacacat gatgattatc tttcgcagat atggctctct ttgctgtttt atctgtctgc
     3241 tgtgcttgtt cgctggagtg ctctcgccgt cagctcatgc cgatggagga gctccccaac
     3301 tcgcctatgt ggcagggaca acccagggca tcagtatcat taatatcgcg cgccggcgtg
     3361 tcactgggac gatggctgtc gctgggaaaa cacacaccgt cttgctgagc cccgatgggt
     3421 acgcgctgta tgtcacgcag cccacgcttg gacgggttgc ggcgatcgcg gcgaaaacag
     3481 ggaagacgc
//
LOCUS       DASZAK010001049         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_418053, whole genome shotgun sequence.
ACCESSION   DASZAK010001049 DASZAK010000000
VERSION     DASZAK010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418053"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..864)
                     /locus_tag="VGT44_18535"
     CDS             complement(<1..864)
                     /locus_tag="VGT44_18535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582862.1"
                     /translation="MRIDTLSYNNVQTQGARRRSWLQQHSPQHLRECAALVLRALELR
                     PAHTSHSTLVLGAGACTEVPLADLARRSDDVILADLDLLSMEKARAELASATMRKATR
                     LVTGDLTGGISPALNRLLERQPWQELISQGAGAVFDAAAQCLDACPVADPPVIEGLYS
                     GECGVVISSLTLTQLFSYPLLDLLDHIQQRAPAFVGEQERHYRYQEAAQSFRTRVIRA
                     HLRLLRTLIDVGGLVVLLSDVRGFAFNSSDAEQDTAQRRTIPLVPRVFPDLVRDTFDV
                     VEERRWEWITDL"
     gene            complement(874..1683)
                     /locus_tag="VGT44_18540"
     CDS             complement(874..1683)
                     /locus_tag="VGT44_18540"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2582863.1"
                     /translation="MRSDYQHLLVQQDGGVLTITMNRPEVLNAFNDVMLTEVTEAMQA
                     AARDEGVRCVVLTGAGRAFGSGQDLRSLSDARASGSLGNETGRVSEHLGNYHTLIVAM
                     RSMPKPVVAAVRGVAAGISCNIALAADMRIVTDDARFIEAFARIGLVPDGGGAYFLTH
                     LVGVGKAMEMAMLADEVSGPEAERLGLVNKCVPAAEFESAVAAFAQRLAHGPTRAYAL
                     IKQQIYAATESDFHSALRLEGELQDMAFETEDHKAAVLAFLQKRKPEYQGR"
     gene            complement(1736..2776)
                     /gene="holB"
                     /locus_tag="VGT44_18545"
     CDS             complement(1736..2776)
                     /gene="holB"
                     /locus_tag="VGT44_18545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV2582864.1"
                     /translation="MNSTREATLEATNQGWGIVGHEHAIDTLRRALLSDRVRHAYLFS
                     GPDHIGKSLLVRRFAQTLLCTGGQDPAGSPQNPCNTCLSCRKVMHNNHPDVHYIARPP
                     DKQFILIDQVRALQGDSARKTMEGRRNIFIITGMHEMNVQAANCLLKTLEEPEPDVVL
                     LLTAPDPGALLPTILSRVQQIPLHLLTTAQIKAALEQFWSVEEREAALVAALAAGRMG
                     WAVQAIEDEDMLANRKTQLEMLARLPMLSRARRFEVAQQLSADGDRLRGILELWLLWW
                     RDLVLAAHNSLDLIVNVDMLALLEKQAAKVGQAEAQRMVRAILGTMEALDQNVNARVA
                     LEVLMLDLPMMR"
BASE COUNT          561 a          902 c          840 g          518 t
ORIGIN      
        1 caaatccgta atccattccc aacggcgctc ctcgacgaca tcgaaggtgt cgcgcacaag
       61 atcagggaac acgcgaggaa ccagtggaat agtacgacgc tgtgcggtat cctgttcagc
      121 gtcagaactg ttgaacgcaa agccccgcac atcgctgagc aagacgacga gaccgccaac
      181 gtcgatcaac gtacgcagca ggcgcaggtg cgcccggatc acgcgcgttc ggaaggattg
      241 cgcagcctcc tggtaacgat agtgccgctc ctgctcgccg acgaatgcgg gagcgcgctg
      301 ctgaatatga tcgaggaggt caaggagagg atagctgaag agctgcgtca gtgtcagaga
      361 actgataacc acaccgcatt cgccgctgta cagaccctcg atgaccggcg gatcggcgac
      421 aggacacgca tccaggcatt gcgcggcggc atcgaagacc gctccggctc cctgcgagat
      481 cagttcctgc cagggctgcc gctccagcag gcggttcagc gccggactga tgccgccggt
      541 cagatcgcct gtaaccaatc gtgttgcttt gcgcatcgtg gcagaagcca gttccgcgcg
      601 ggccttctcc atcgaaagca gatcaagatc ggccagaatg acatcatcgg agcggcgcgc
      661 cagatcggcc aggggaacct ccgtgcaggc ccccgcgccc agcacaagcg tgctgtgcga
      721 ggtatgcgcc gggcgcagtt caagggcgcg cagcacgagc gcagcgcact cgcgcaggtg
      781 ctgcgggctg tgttgttgca gccagctacg ccgccgcgcc ccctgggtct ggacgttatt
      841 ataggaaagg gtatcgatgc gcacgaatcc gacctatcgc ccctgatatt ctggtttgcg
      901 cttctgcaag aaagccagca cggcagcctt atgatcttcc gtctcgaagg ccatatcttg
      961 caactcacct tccaggcgca gagcggaatg gaaatcggac tccgtcgcgg catagatttg
     1021 ctgcttaatc agcgcgtagg cgcgcgtcgg gccatgcgcg agacgctgcg cgaaagcggc
     1081 aaccgcgctt tcgaattccg ccgccgggac gcatttgttg accaggccca gccgttcggc
     1141 ctccgggccg ctcacctcgt cagccagcat cgccatctcc atggccttgc ccacaccgac
     1201 aagatgcgtc aggaaatatg cgccgccgcc atcgggaacc agcccgatgc gcgcgaaggc
     1261 ttcgatgaag cgcgcatcat cggtaacaat gcgcatatcg gctgccagcg cgatgttgca
     1321 cgagatgccc gccgcgacgc cgcgtaccgc cgcgacgacc ggtttgggca tcgagcgcat
     1381 cgccacgatc agcgtatggt aattccccag gtgttcgctg accctgcccg tctcgttgcc
     1441 aagcgagcca ctggcgcgtg cgtcggacag actgcgcagg tcttgccccg acccgaaggc
     1501 ccgtcccgca cccgtcagca caacgcagcg cacaccctca tcacgcgcgg cggcctgcat
     1561 cgcctccgtc acctcggtca gcataacatc gttgaacgca ttcaatactt ccggtcggtt
     1621 catggtgatg gtcagtacgc caccatcctg ttgcacgagc aggtgttggt aatcgcttcg
     1681 caaaagactc gcctcctttg agacttgcgg gaatgaaatt ctaaaattta cctgattacc
     1741 tcatcatagg caaatccagc atcagcacct caagcgccac gcgcgcattt acgttctgat
     1801 ccagggcctc catcgttccc aggatggcgc gcaccatgcg ctgtgcttcg gcctggccta
     1861 ccttcgcagc ctgcttttcc agcagggcga gcatatcgac gttgacgatc aggtcaaggc
     1921 tgttgtgagc ggctagaacc aggtcgcgcc accagagcag ccacaactca agtataccac
     1981 gcagccggtc gccatcggcg ctcaattgct gcgcgacctc gaaccgccga gcgcggctga
     2041 gcataggcag tcgcgccagc atctccagtt gcgtcttgcg attggcgagc atatcctcgt
     2101 cttcgatggc ctgtacagcc cagcccatgc ggcccgccgc cagcgccgca accagcgcgg
     2161 cctcccgctc ctccaccgac cagaattgct ccagcgcggc cttgatctgc gccgtcgtga
     2221 gcaggtgcag gggaatctgc tgcacgcgcg acaggatggt gggcagcagc gcccctggat
     2281 cgggcgcggt gagcagcagc acaacatccg gctccggctc ttccagtgtt ttgagcaggc
     2341 agttggccgc ctgcacgttc atctcgtgca ttccggtaat gatgaagata ttgcggcggc
     2401 cctccatcgt cttgcgcgcg ctatctccct gcaaggcgcg tacctggtcg atcagaataa
     2461 actgtttatc gggcgggcga gcaatataat gcacgtcggg atggttgtta tgcatcactt
     2521 tgcggcagga gaggcaggta ttgcacggat tctgcggcga gcctgccggg tcctgcccgc
     2581 cggtgcagag caaggtctgc gcgaaacgcc gcacgagcag gctcttgccg atatgatccg
     2641 ggccgctaaa aaggtaagca tgacgcaccc ggtcggagag cagtgcgcgg cgcaacgtgt
     2701 cgatggcgtg ttcgtgtcca acgatgcccc atccctggtt ggttgcttcg agcgtagctt
     2761 cgcgcgtgct gttcatggcg tcatagtagc agatagcgcg aagaatgaca agctaccagg
     2821 g
//
LOCUS       DASZAK010001050         1933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_632795, whole genome shotgun sequence.
ACCESSION   DASZAK010001050 DASZAK010000000
VERSION     DASZAK010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632795"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VGT44_18550"
     CDS             complement(<1..484)
                     /locus_tag="VGT44_18550"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2582865.1"
                     /translation="MDNLPLVNLVRQPLPEASNAPLLLLLHGVGSNEHDLFGLAPLLD
                     QRFIVLSLRAPNTLGPGSYAWFEVAFTPQGPVINPEQAEQSRQLLIDFIGQAVATYGA
                     NPRQVYLMGFSQGAIMSASVALTRPDLVAGAVLMSGRILPEIQPLVATPEQLKGLPVL
                     V"
     gene            complement(500..694)
                     /locus_tag="VGT44_18555"
     CDS             complement(500..694)
                     /locus_tag="VGT44_18555"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HEV2582866.1"
                     /translation="MPIIRIEMLEGRPPAVKTELIRRVTDAVVETLKVAPEQVRVLLY
                     ELPPQHWGVGGKTKAETEKS"
     gene            complement(721..1329)
                     /locus_tag="VGT44_18560"
     CDS             complement(721..1329)
                     /locus_tag="VGT44_18560"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2582867.1"
                     /translation="MRDIPEPIPLKAQHCKQASGVLGRAFHQDPMMRLIVPDDAKRAR
                     LLPSFFAIAARYCLFYGEGYITPELDGVACWLRPGDTTPGIGRLVRIGIRGAPVGIGL
                     PGLRRFMDVSRYADEVHARCVPGKHWYLWCIGVDPARQGQGIGGRLMQPVLAKASAEG
                     VPCYLETMNEANLPFYEKYGFIVVNDGVVPRHGLRAWAMLRK"
     gene            complement(1336..>1933)
                     /locus_tag="VGT44_18565"
     CDS             complement(1336..>1933)
                     /locus_tag="VGT44_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2582868.1"
                     /translation="CIEHDVVVISDEVYEHLLYDEAEHVPIATLPGMFERTLTISSAG
                     KLFSATGWKIGWMFGPPEMVEGPARAHMFVTFAVHHPTQEAIAYALNLPDSYYSSYKA
                     MYARKRGFMLSALERAGFTYCVPQGAYYVMADYSRIFDGTPLECVHYLTREVGVACIP
                     PESFYSREHTSIGQSYIRFAFCKTDEMLEQAQERLARK"
BASE COUNT          380 a          623 c          549 g          381 t
ORIGIN      
        1 cgaccagcac cggcagcccc ttgagttgct caggcgtcgc cactaggggc tgtatttccg
       61 gcaggatgcg cccgctcatc agcaccgcgc cagccacgag atcaggccgc gtcagcgcga
      121 cactggcgct catgatcgcc ccctggctga agcccatcag gtagacctgg cgcggattcg
      181 cgccataggt cgcgaccgcc tgcccaataa aatcgatcaa taactggcgg ctctgctcgg
      241 cctgctccgg gttgataacc ggcccctgcg gcgtgaaggc aacttcaaac caggcatagc
      301 tgcccggtcc taacgtgttc ggggcgcgca ggctgagaac gatgaagcgt tgatcgagca
      361 gcggggccag cccgaacaaa tcgtgctcat tgctgcccac cccgtgcagc agcagtaaca
      421 gtggcgcatt actcgcctcc ggcagcggct gtcgcacgag atttacaagt ggtagattgt
      481 ccaatgaata tctcctcttc tacgatttct ccgtctcggc tttcgtcttt ccgcccacac
      541 cccaatgctg cggcggcagc tcgtagagca ggacgcgcac ctgttccggc gcaaccttga
      601 gcgtctcgac tacggcatcg gtgacgcgcc ggatcaattc ggtttttacg gcgggcggac
      661 gcccttccag catctcgata cggatgattg gcatactatt cttccctcac tattacctgg
      721 ctacttgcgc agcatggccc aggcgcgcag gccgtgtcgc ggcacaacgc catcgttgac
      781 tacgatgaac ccgtactttt cgtaaaacgg cagattggcc tcgttcatcg tctccaggta
      841 gcacggcaca ccctccgcgc ttgccttcgc cagcacgggc tgcatcagtc gcccgccgat
      901 accctggccc tggcgggccg ggtcgacgcc aatgcaccac aggtaccagt gtttacccgg
      961 aacgcagcgc gcatgtacct cgtcggcata tcgtgacacg tccatgaagc ggcgcaatcc
     1021 tggcaggcca atcccaaccg gtgcgccgcg tatccctatc cgcaccaggc gacctattcc
     1081 tggcgtagta tcgcctggtc gcagccagca ggccacgcca tccagttctg gcgtgatgta
     1141 gccctcgcca tagaagagac agtagcgcgc agcgatagcg aagaacgaag gcagcaggcg
     1201 cgcgcgtttc gcatcatccg ggactatcag acgcatcatc ggatcttgat gaaacgcgcg
     1261 gcctagcaca ccggatgcct gcttgcaatg ctgtgccttg agcggaatgg gttctggaat
     1321 atcgcgcatg tcgttctact tccgcgccag tcgttcctgc gcctgttcca gcatctcgtc
     1381 ggttttgcag aaggcgaagc ggatatagct ctgcccgata gaggtgtgtt cccggctgta
     1441 gaacgactcc ggcgggatgc aggccacgcc gacctcgcgc gtcaggtaat gcacgcattc
     1501 gagcggcgtt ccatcgaaga tgcgcgaata atccgccatg acgtaatagg ccccctgcgg
     1561 cacgcaatag gtaaacccgg cgcgttccag ggccgagagc ataaaaccgc gttttctggc
     1621 atacatagcc ttgtatgacg aataatatga gtcgggcagg ttgagggcat aggcaatcgc
     1681 ctcctgtgtg gggtgatgca cggcgaaggt tacgaacatg tgcgcgcgcg ccgggccttc
     1741 taccatctcc ggcggcccaa acatccagcc gattttccag cccgtcgcgc tgaacagctt
     1801 gcctgcgctg ctgatcgtca gcgtccgctc gaacatgccc ggcagcgtgg cgatgggtac
     1861 atgctcggcc tcgtcataca gcaaatgttc gtacacctcg tcggagataa ccactacatc
     1921 atgctcgatg cac
//
LOCUS       DASZAK010001051         3020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_702450, whole genome shotgun sequence.
ACCESSION   DASZAK010001051 DASZAK010000000
VERSION     DASZAK010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702450"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..616
                     /locus_tag="VGT44_18570"
     CDS             71..616
                     /locus_tag="VGT44_18570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEV2582869.1"
                     /translation="MTSPAEQTSGRILIACVGNIFLGDDGFGVEVARGLIGRKYPEGV
                     QVIDFGIRGMELAYTLLDDYDALVLVDCISRGGTPGTLYLIEPDLAGVVPENGAEAGR
                     AALDAHSMDPAKVLAFASVLGARPIRTLIVGCEPAKIITGEDDTDMQMGLSEPVQAAV
                     GEAVKMIDSLVGRLLASTPVT"
     gene            824..1174
                     /locus_tag="VGT44_18575"
     CDS             824..1174
                     /locus_tag="VGT44_18575"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEV2582870.1"
                     /translation="MHELAIAQSIVDIVETRAAECHATHVKGVRLRIGEAIGVVTDSL
                     TFCFELLTSLDPTLAGAQLSIDTVPHQAQCRHCAREFPVVNFIAQCPVCKEWSSDVVS
                     GTELQILEMEYEAQ"
     gene            1307..2011
                     /gene="hypB"
                     /locus_tag="VGT44_18580"
     CDS             1307..2011
                     /gene="hypB"
                     /locus_tag="VGT44_18580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEV2582871.1"
                     /translation="MTIAQNILAANDTIAQQIQQSLAALNIRTINIMSSPGAGKTTLL
                     ERTIERLSGQLGIAVIEGDVETSADAERIEAAGAETVQINTRGACHLEAHMIRDALAQ
                     IDLERVDLLVIENIGNLVCPSGWNLGEDLRVVVASTTEGDDKPAKYPQMFAVSQVMII
                     NKLDLLPYLDYDVEKVKRQALAINPHLRIFELSCRTGAGLEAWCDWLIAFVRSEAQQP
                     AKGALREEHSLGISQR"
     gene            2025..>3020
                     /locus_tag="VGT44_18585"
     CDS             2025..>3020
                     /locus_tag="VGT44_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HEV2582872.1"
                     /translation="MSSVIGSTIQRQRIVIQGIVQGVGFRPFVYGQALRWNLVGFVLN
                     DSNGVTIEVEGPPDALDSFQRALREEIPPLARIDSIFTELVPVLHDSAFAITHSQAGI
                     ERNTLISPDTATCSDCLHELFDPVDRRYRHPFINCTNCGPRFTIVQDVPYDRDKTTMR
                     IFPMCAACRAEYDDPLNRRFHAQPNTCPDCGPHVSLLDWTCRGDPRDRPATLDPIAAA
                     AQRLASGGILAIKGLGGYHLACDALNAEAVRKLRQQKHREAKPFALMVPDLETAQWLC
                     HVSDAEAELLHSRRRPIVLLSKRPDCPVAPDVAPAYDSLGIMLPYTPLHHLLLRAF"
BASE COUNT          682 a          810 c          836 g          692 t
ORIGIN      
        1 gtgttctccc tggtcaccgt ttctacttct ttcccgatga agtcgagccg ttaggggaga
       61 agccctgagt atgacatctc cagcagaaca gacatccggg cgcatactga tcgcgtgcgt
      121 tggcaatatt ttcctgggtg atgacggttt cggagtggag gtagccagag gcctgatagg
      181 tcgtaagtat ccggagggcg tgcaggtcat cgatttcggt atccgcggta tggaacttgc
      241 gtatactctg ctcgatgact atgatgcact cgtcctggtc gattgcattt cacgcggagg
      301 aacgccggga acactgtatc tcatagaacc cgacctcgca ggcgtggttc ctgagaacgg
      361 agcagaagcc ggtagggctg cactggacgc ccatagtatg gacccggcga aggtgctcgc
      421 cttcgcaagt gtcctgggag cgcgtccaat ccgcaccttg attgttggct gcgaaccagc
      481 taagattatt acaggtgaag atgacactga catgcagatg ggattgagcg aacccgtaca
      541 ggctgccgtc ggtgaggcag taaagatgat cgactcgctc gtcgggcggt tgctcgcgtc
      601 aacaccagtc acgtaacagt ctgtgaacaa aaaaagaaag gaacattgct atgttcaaat
      661 ggcttgccct ggctatggtc attgcacttg gatacgtagc atatcgatca ctaccggatg
      721 tccagcgtta tctgaaaatg cgtaatatgt aatggaacaa gagtgtccgc aagggtacag
      781 taagcttcgc ccagaatagt gcattcgagg agtgaggttg gatatgcacg agcttgcaat
      841 agctcaaagt atcgttgaca ttgttgaaac tcgagcagcc gagtgtcatg ccacacacgt
      901 gaagggtgtg cggctgcgca ttggcgaggc catcggtgtc gtcaccgact cgttgacttt
      961 ctgctttgag ttgttgacca gcctggatcc aaccctcgca ggcgcgcaac tgtcgatcga
     1021 taccgtacct caccaggcgc aatgtcggca ctgcgccaga gaatttcccg tcgtcaactt
     1081 tattgcccag tgtcccgtgt gtaaagaatg gtccagcgac gtagtttctg gtaccgaact
     1141 gcaaatcctt gagatggagt atgaagccca atagggactt gattaatcat gctcatcgcc
     1201 gattatagct ttaaaaaatt ttccagacat ctgtaggtgc cgatttatcg gcccttgtgg
     1261 atgttctgaa tcaaccccac cggagggagt ggcgactttg cccaaagtga caatagccca
     1321 aaatatactg gcagctaacg atacaattgc ccagcagatc cagcagtcgt tggcggcgct
     1381 caacattcgc acgatcaaca tcatgagctc gccaggcgct ggtaagacga ccttgctgga
     1441 gagaactatc gagcgactca gcggacaact cggcattgct gtgatcgagg gcgacgtcga
     1501 gacaagcgct gacgcggaac gtatcgaggc agccggggcc gagacggtcc agatcaacac
     1561 gcggggagca tgtcatcttg aggcgcatat gatccgcgat gcactcgcgc agatcgacct
     1621 ggagcgcgtc gacttacttg tcatcgagaa cattgggaac cttgtatgcc cctccggatg
     1681 gaatctggga gaagacttgc gcgttgttgt tgcgagtacc accgaaggcg acgacaagcc
     1741 tgcaaagtat cctcaaatgt ttgcggtctc acaggtaatg atcattaata agcttgatct
     1801 gctcccttat ctagattatg acgtggagaa ggtcaaacgg caggcgttag ctatcaatcc
     1861 acacctgcgc atcttcgagc taagttgtcg gacgggcgcg ggactcgaag cgtggtgcga
     1921 ctggcttatc gcatttgtgc gtagtgaagc gcagcaacct gcaaagggtg ctcttagaga
     1981 agaacactcg ctagggatat cccaacgttg aaaaggtggt atgtatgtca tcggtaatag
     2041 gttcgaccat tcagcgacag cgcatcgtca tccagggcat tgtccagggc gtaggcttcc
     2101 ggccttttgt ctatgggcag gcgctgcgtt ggaatctggt cggttttgtg ctgaatgaca
     2161 gcaacggtgt gaccatcgag gtcgaaggac cccccgacgc gctcgacagc tttcagcgtg
     2221 cgttgcgcga agagatccct cccctggcgc ggatcgattc tattttcacc gaacttgtac
     2281 cggtgctcca cgatagcgcc ttcgcgatca cacacagcca ggctggaatc gagcgcaata
     2341 cgctcatttc gccggatact gccacctgta gcgactgttt gcatgaactc ttcgaccctg
     2401 tagaccgtcg ttatcgtcat cctttcatta actgcaccaa ttgcggcccg cgcttcacca
     2461 ttgtccagga tgttccctat gaccgtgata agacaaccat gcgtatcttc ccaatgtgcg
     2521 cggcctgccg ggcggagtat gatgatcccc tgaaccgccg ttttcacgcc cagcccaaca
     2581 catgtcctga ctgtggcccc catgtcagcc tgctcgattg gacatgtagg ggcgaccctc
     2641 gcgatcgccc ggcaactctt gaccccatcg ccgcggcagc acagcgcctc gcttcaggcg
     2701 gcattctcgc gataaaagga ttgggaggtt accacctggc ttgcgatgca ttgaacgcgg
     2761 aggcagtacg caagttgcga caacaaaagc atcgcgaggc taaaccattc gcgctcatgg
     2821 tgcctgacct ggagacggct caatggctct gccatgtgag cgatgccgag gcggagctac
     2881 tccactcgcg cagacgtcct atcgttctct tgagcaagcg tcctgattgt cctgtggctc
     2941 cagacgtcgc gccagcttat gacagcctgg gaattatgct tccctacaca ccactgcatc
     3001 accttctttt gcgcgctttc
//
LOCUS       DASZAK010001052         4232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_27209, whole genome shotgun sequence.
ACCESSION   DASZAK010001052 DASZAK010000000
VERSION     DASZAK010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27209"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..558)
                     /locus_tag="VGT44_18590"
     CDS             complement(196..558)
                     /locus_tag="VGT44_18590"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV2582873.1"
                     /translation="MQDDALTRLREICLALPESSEQGGVGNPSFKVRDKIFAMRHGFD
                     GRMSMWCKAPPGAQEVLVGSNPARFFVPPYVGHHGWIGLWLDGEIDWDEVADLVRESY
                     RMTAPKRLLAMLDRTNSS"
     gene            779..1615
                     /locus_tag="VGT44_18595"
     CDS             779..1615
                     /locus_tag="VGT44_18595"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2582874.1"
                     /translation="MKTEQSAEHDVAALNQQVRAIWNQNAGFWDEKMADEGNDFQKLL
                     IGPAAERLLNLQAGELVLEVGCGNGVFARRMAALGAQVIATDFSEAMIERAQARTVDH
                     ADRLEYRVVDATSEEQLLSLGRQRFDAIVCLMLIMDMAAIEPLMRAVGQLLKPQGRFV
                     FSIMHPCFNSNGALMMAEEHVEDGNYIVDYAIKVKEYLDVPVQTGIGIAGQPTPHYYF
                     HRPLHVLLGTCFNGGLVMDGIEEPAFHHQNDGSKPSTRALGWVNYQQIPPVLLARMRL
                     LS"
     gene            1638..2528
                     /locus_tag="VGT44_18600"
     CDS             1638..2528
                     /locus_tag="VGT44_18600"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2582875.1"
                     /translation="MLSIKDETFEGTFPFAPHYYSAGDFDMHYVDEGSGEPVVMLHGD
                     PTWGYLYRNFISPLSRRARCIVPDHMGMGKSSVPPERGRYRLEQHCANLEALLLHLDL
                     RDITLVLHDWGGPVALGFAIQHPDRIKRLVLTNTWAFAPWPGGPFPRLLELIRSERGE
                     AFVLQKNGYLAPALQGTTFHRERLTPEVMQAYQAPYPTPESRLAMLCWSRDIPVQESD
                     VSFAAMKRIEQGLSGFNDIPVLLIWGMQDPVLPAPVLQRWQQLYPHATTHVLEDASHF
                     LQEDAPERIVPWIEAFINAT"
     gene            2746..3627
                     /locus_tag="VGT44_18605"
     CDS             2746..3627
                     /locus_tag="VGT44_18605"
                     /inference="COORDINATES: protein motif:HMM:NF037411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582876.1"
                     /translation="MKHIIFSRKGFDASVGKVASPILPSGELCSLPIPEPRSYDGAPQ
                     YAGLRVGEHSLGKLVGDLSRGKITPDLATHLDPDLRKESLPRQPGWKPLFGQAGAAER
                     HLQNRHVQPGDLFLFYGWFRRVEQCNGAYRYVPGAPDLHVLFGWLQIERRIPVAQGED
                     IPSWATYHPHSRRSQVGLPYSTLDSLYIATEHLHLPGASPTLPGAGVFRHYHPALCLT
                     HTDPFTSRRLWQLPSWMYPAQGKTPLTYHPSNTLWQRATDGSTLLRTAGRGQEFVLNC
                     DEYPQALEWVAQLLTCH"
     gene            complement(3621..4055)
                     /locus_tag="VGT44_18610"
     CDS             complement(3621..4055)
                     /locus_tag="VGT44_18610"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV2582877.1"
                     /translation="MAHAEESITINRQVDTVFNFVLDGMNNPRWRPAVIDIQQAPGKP
                     SGLGAVYKQGLKGPGGRRIDGDYEIVKCQPNELIEFQVIAGPARPTGMYTFATEGNST
                     RITFTLHFEPKGLARLMNSMITRTMQSEVATLSNLKAYLESQ"
BASE COUNT          871 a         1280 c         1177 g          904 t
ORIGIN      
        1 agttagaaca ggcgagtgtc tcagacatct tcatcgccga tgaactaacg catccagaac
       61 gcctcaaagc gcacctcgac aaagtcatcg accggcatca atgatgtcaa cactctaatt
      121 tcatcgtagg gacgcgaatg atcgcgtcct ggtggccttg tacgaccgag gacgaggact
      181 cgatcattcg cgtccctacg acgaatttgt gcggtccaac atcgctaaca ggcgctttgg
      241 ggccgtcatg cgatagctct cacgcaccag gtccgcaacc tcgtcccagt cgatctcgcc
      301 gtcaagccac aggccgatcc agccatgatg gccgacgtag ggcggcacga agaagcgcgc
      361 agggttggag ccaaccagta cctcctgcgc gcccggcggg gccttgcacc acatggacat
      421 gcgcccatca aagccgtgcc gcatggcgaa aatcttgtca cgtaccttga acgatggatt
      481 ccctacgcct ccctgctctg acgattccgg cagcgccagg catatttcgc gcagccgcgt
      541 gagtgcatca tcttgcatac tgtatccttt caggggatta tcgccaacca accaacaccg
      601 attctgtcat cctgagcgca gcgaaggatc tcggtgtcgg gcacatcaga cccttcgctg
      661 cgctcagggt gacaacacca taccgatctt gtttgtgaaa actcataatc ggcgacgggc
      721 gcggtgataa aatatcataa acgtctctat cctattctac ataacgaaag agccagaaat
      781 gaaaaccgaa caaagtgcag agcatgatgt tgcggcgctc aaccagcagg tgcgagcgat
      841 atggaaccag aatgcaggct tctgggatga gaagatggcc gatgaaggaa acgattttca
      901 gaaactcctt attggacctg cggcagagcg gcttctgaac ttgcaggcgg gggaactggt
      961 attggaggtc ggatgcggca acggcgtctt cgctcgccgc atggccgcgc ttggggcgca
     1021 ggtgatcgcc accgatttta gcgaggcgat gattgagcga gcgcaagcgc gaacggttga
     1081 ccatgctgat cgcctcgaat accgcgttgt cgatgccacc agtgaagaac aactcctctc
     1141 gttgggcagg cagcgctttg acgccatcgt ctgcctcatg ctcataatgg atatggccgc
     1201 gattgagccg ttgatgcgcg ccgttgggca attactcaag ccgcaggggc gtttcgtttt
     1261 ctcgatcatg catccctgtt tcaactcaaa tggcgctttg atgatggccg aggagcatgt
     1321 cgaggatgga aactatatcg ttgactatgc catcaaggtg aaggagtatc tggatgtgcc
     1381 ggtgcaaacg ggcatcggca tcgccgggca acccactccc cattattatt tccacagacc
     1441 gctgcatgtc ctgctgggaa cctgtttcaa cggcggcctg gtgatggatg gcattgaaga
     1501 accggccttt catcaccaga acgatggttc taaacccagc acccgcgctc tgggctgggt
     1561 aaattaccag cagattccgc cggtgctgtt agccagaatg cgcctgttgt cataactgac
     1621 tggagatact caaatctatg ctatccatca aagacgaaac gtttgaggga accttcccgt
     1681 ttgcaccgca ttactactct gctggcgact tcgacatgca ctatgtagat gagggaagcg
     1741 gcgagccggt cgtgatgctg cacggcgacc cgacgtgggg ctacctctat cgcaacttca
     1801 tctcgccgct ttccaggcgc gcgcgctgca tcgttcccga ccacatgggc atgggaaagt
     1861 cttccgttcc gccagaacgg gggcgttacc gcctggaaca gcactgcgcc aaccttgaag
     1921 cgctgctgct gcatcttgac ctgcgcgaca tcacgctcgt cctgcacgat tggggcggcc
     1981 cggtcgcgct tggttttgcc atacagcacc ctgaccgcat caaacggctc gtgctgacga
     2041 atacctgggc gttcgctccc tggccgggcg gaccttttcc gcgcctgctt gaactgatcc
     2101 gttcagaacg cggagaagcc ttcgtgctgc aaaagaacgg ctacctcgcg cccgccttgc
     2161 agggcacgac cttccaccgc gaacggttga ctccggaggt catgcaggca taccaggccc
     2221 cttatccaac gccagaatcc cgccttgcca tgctctgctg gtcgcgcgat atcccggtgc
     2281 aggaaagcga tgtgtccttt gccgccatga agcgtatcga gcaaggtctg tccggcttta
     2341 atgacattcc tgtgttgctg atatggggca tgcaagaccc tgtcctcccc gcgccggtct
     2401 tacagcgctg gcagcaactc tatccgcacg ccacaaccca cgtactggaa gatgcaagtc
     2461 acttcctaca agaagacgcc ccggagcgca ttgtgccgtg gatcgaggca ttcattaacg
     2521 ctacatagtt tttccagggc gaccgccgtg tgtggcctgt tgtcggatgt caccctgagc
     2581 gcagcgaagg gtcttcctcg ccgagtgccg agatccttcg ctgcgctcag gatgacacgc
     2641 aacgagggta ggggcgatgc tagccatcgc cctggtggaa atgcgccgct caggatgaca
     2701 cgcaaagagg gtaggggcga tgctggccat cgccctggaa aaactatgaa acacattatc
     2761 ttcagtcgca aaggctttga cgccagcgtt ggcaaggttg ccagtcctat cctgccgtcg
     2821 ggcgaactct gctcgctgcc catcccggaa ccacgcagct acgatggcgc gccgcagtat
     2881 gccgggctgc gtgtcggtga gcattcgctg ggcaagctgg taggcgacct gtcgcgcggc
     2941 aaaatcacgc ccgacctcgc tacacatctc gaccctgatc tgcgcaaaga gagccttccc
     3001 cggcaacctg gctggaagcc gctcttcggg caggccgggg cagccgaacg ccatctccag
     3061 aacaggcatg tgcagccggg cgatcttttc ctcttctacg gctggttccg gcgggtcgag
     3121 caatgcaacg gtgcttatcg ctatgtccct ggcgctcccg atttgcacgt cctcttcggc
     3181 tggctgcaaa tcgagcggcg tatccctgtt gcgcaaggcg aggatatccc atcatgggca
     3241 acctatcacc cacattcccg acgctcgcaa gttggtttgc cttactcaac tctcgattca
     3301 ctttacatcg cgaccgaaca cctgcatctg cctggcgcgt cgccaactct ccctggcgcg
     3361 ggcgtctttc gccattatca tcccgccctc tgcctgacgc atactgaccc attcacatca
     3421 aggcggctgt ggcagcttcc gtcatggatg tatcccgcgc aaggcaaaac gccgctgacc
     3481 tatcacccca gcaacacgct atggcagcgc gccacggatg gttcaacgct gctcaggacg
     3541 gcaggacgcg gccaggagtt cgtgctgaac tgcgacgagt acccgcaggc gctggagtgg
     3601 gtggcgcagc ttctcacgtg tcactgactc tccaggtaag ctttaagatt ggaaagcgtt
     3661 gcgacctcgc tctgcatagt gcgggtaatc atagagttca tgagcctggc cagccctttc
     3721 ggctcgaaat gtagggtgaa ggtgatgcgg gtcgagtttc cctcggtcgc aaatgtgtac
     3781 atacccgtgg gccttgccgg tccggcgatg acctggaact cgatcagctc gttcggctgg
     3841 cacttgacga tctcgtagtc gccatcgatg cgcctgccgc caggcccttt cagcccctgt
     3901 ttgtagaccg ccccaaggcc cgatggtttg cccggcgcct gctgtatatc gattacggca
     3961 ggtcgccagc gtgggttgtt catgccgtcc agaacgaaat tgaacacggt atccacctgt
     4021 cggtttattg tgatgctttc ttccgcgtga gccatgatct gtctcctttc aaatccaatc
     4081 gaaacgttgt acatcgtcga ccggtgtagg ggcgcaattc attgcgccct ggtggaggcg
     4141 cgtcacccgg accgagggcg caatgaattg cgcccctaca ccggtcactt ccaccttgac
     4201 aaagcagtag ttgtttcgta acgctcctac ct
//
LOCUS       DASZAK010001053         2289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_99653, whole genome shotgun sequence.
ACCESSION   DASZAK010001053 DASZAK010000000
VERSION     DASZAK010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99653"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VGT44_18615"
     CDS             complement(<1..751)
                     /locus_tag="VGT44_18615"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2582878.1"
                     /translation="MDFELPEEHQLAYESAFAFAQDEIRPHNAEIERTDDFPPWIWKR
                     LAQQGYTGIAIPEEYGGSGGDFLMAALVARAIGRANSGISMSYGAHLNLCAHNILRNG
                     TEAQKESYLPGMASADMIGGLGLTEPNAGSDAMGIQMTARETSGGYLLNGTKVFITNG
                     PIAHLLIVYAKTRPDLGSRGITAFIFETNTPGYHVARKLEKVGMHGSPTGELVFEDAF
                     VPAENVLGKVNEGYKVVMSGLDLERAFYAVSM"
     gene            1044..>2289
                     /locus_tag="VGT44_18620"
     CDS             1044..>2289
                     /locus_tag="VGT44_18620"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2582879.1"
                     /translation="MSSGVPSNPQRIGKYELRERLGSGGMAEVWKAYDPQLERFVAIK
                     ILHPDLQTDPEFLTRFVREARVSASLHHPNIIQIHDFQTIQSADASSPMAYMVMDYIE
                     GPTLARYIRSTSCVGKFPTADEIVQLFASISRAVDYAHQHNMIHRDIKPANILLDQRN
                     RMQNPMGEPILTDFGIARIIGISSGTASGGWLGTPIYIAPEQALGQPGTERSDIYSLG
                     VILYEICTGVQPFRGENISSLMMQHIHTAPTAPSLINPNIPPALSMVILRCLNKDPMN
                     RFSSAAALAAALGEALNVQLPADISQHWMLAEEMSQPTMLSPLRPGTPISITPLLPPS
                     SLPGTVQLAQSGSQMVSPLPAQVGTKPINPTTPFPGSFTPTSSATGQQAALLSPPFVN
                     APPLQTPVFAQPDPPVKKQRRGM"
BASE COUNT          469 a          642 c          646 g          532 t
ORIGIN      
        1 ccatgctgac ggcgtagaag gcgcgttcga gatcgaggcc gctcatgacg actttgtagc
       61 cctcgttgac cttgcccagc acgttttcgg cgggtacgaa ggcgtcctcg aagacgagtt
      121 cgccggtggg agagccgtgc atcccgactt tttcgagctt gcgtgccacg tgatagcccg
      181 gcgtattcgt ttcaaagatg aaggccgtga tgccgcgact gcccaggtcc gggcgcgtct
      241 tggcgtagac gatgagcagg tgggcgatgg gaccgttggt gatgaagacc tttgtgccgt
      301 tgagcaggta gccaccgctc gtctcacgcg ccgtcatctg aatgcccatg gcgtctgagc
      361 cggcgtttgg ctcggtcagc cccagtccgc cgatcatgtc ggcgctggcc atgccgggca
      421 ggtagctctc tttctgcgcc tcggtgccgt tgcgcaggat attgtgggcg cacaggttca
      481 ggtgcgcgcc gtaggacatc gatatgccgc tgttggctct gccgatggcg cgcgccacga
      541 gcgccgccat taaaaaatcc ccgccgctgc cgccgtactc ttccggaatg gcgatgccgg
      601 tatagccctg ttgcgccagc cgcttccata tccagggtgg gaaatcatct gtgcgctcga
      661 tctccgcgtt atggggccgt atttcgtcct gggcaaaggc gaaggcgctt tcgtaggcaa
      721 gctggtgttc ttcgggcagt tcaaagtcca tggggcagag gtcctttctg gcgtaggtga
      781 cgagacacga tgctgcgact aatcgtaagt ttacgttctg attggagaga atgcaagcct
      841 atttcttcga gcaacgtaac gctcaggttt tagaaaaaat cctgtaaaaa cgcgcattga
      901 caacgtcttt atctattgat tattatggta gtggtataag catctgttat cctcttcgtc
      961 gtgtattgct tgcttgttgc tcgaacgtcc gtgagttcat tggtgataga gaccggagct
     1021 aagcttcaga aggggaacct gtcatgagca gtggcgtacc ttccaatccg cagcgcatcg
     1081 ggaaatacga gttgcgcgaa cgtttgggga gtgggggaat ggccgaggtg tggaaagcct
     1141 acgatccaca acttgagcgt tttgtcgcta tcaaaatcct tcatccagat ctgcagaccg
     1201 atcctgagtt cctgacgcgc ttcgttcgcg aggcacgtgt cagcgcctca ctgcaccacc
     1261 cgaacatcat acagatccac gattttcaga cgatccagtc tgccgatgcg agcagtccta
     1321 tggcatatat ggtaatggat tatatcgagg ggccgacact tgctcgttat attcgttcaa
     1381 cctcgtgtgt gggtaagttc cccactgccg acgaaatcgt gcagttgttc gcgtctatca
     1441 gccgggcggt tgattacgct catcagcaca atatgatcca tcgcgatatc aagccggcca
     1501 atattctact agaccagaga aatcgcatgc aaaatccgat gggcgagccg attctgaccg
     1561 atttcggcat tgccaggatc atcggcatca gtagcggcac agcaagcggc ggctggttgg
     1621 gtacgcccat ttatattgcg cctgagcagg ctctgggcca acctggcaca gagcgcagcg
     1681 atatctattc gctcggagta atcctgtacg agatatgtac cggagtgcag ccattccggg
     1741 gggaaaatat ctcttccctg atgatgcagc atatccatac ggcgcctact gcgccttcgc
     1801 tgatcaatcc caacattccg cctgccctgt ccatggtgat tcttcgctgc ctgaataagg
     1861 acccgatgaa ccgtttcagc agcgctgctg cgctggcggc tgcgctggga gaagcgttga
     1921 atgtgcaact cccggcggat atcagccagc attggatgct ggcggaagaa atgagccagc
     1981 cgaccatgct ctctccgctt agaccaggca cacctatcag cattacacct ctattaccgc
     2041 caagttcatt gcctggcacg gtgcagcttg cccaatccgg gtcgcagatg gtatcgcctt
     2101 tgcccgcgca agttgggaca aagccaatta accctacgac tccctttcca ggaagcttta
     2161 cgccaacatc ctcagcgact ggccagcaag cggcgctcct gtcgcctcca ttcgtcaacg
     2221 cgcctccatt gcagaccccc gtctttgctc agcccgatcc gcctgtaaag aagcagagaa
     2281 gggggatgg
//
LOCUS       DASZAK010001054         2104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_132307, whole genome shotgun sequence.
ACCESSION   DASZAK010001054 DASZAK010000000
VERSION     DASZAK010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132307"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VGT44_18625"
     CDS             <1..1051
                     /locus_tag="VGT44_18625"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEV2582880.1"
                     /translation="STRHVRASTLALCRAMAGADAPGLIDGPGDFKDETLVDMAVIAA
                     RRALASAGRDASEIDTVIGASSSDNDAFPTIAGQVQYRLGLGPIRATMLKGACACQTE
                     AFQTCAEILAASSAKLVLMVTAEGLLPNIMNVLDWKTSSLFGEGASAYLLEIGDEDTY
                     VINGSDAAQAPALYYQTPLRKDVLDMAEVDLKMRQHYAAGNGKELNAILAQYHVGYTK
                     MNGKEVFREAPRAMAEAIDALCRHAGLSHDELAHIIPHQANSRITRRMGELLIHDYGW
                     PSSTMEKLVDNFRYYGNLSNASIATAMAELLRKSNLHDGEWMALPAVGGGMNYGCWLL
                     RYHGLAHPEAVMGDG"
     gene            complement(1048..1932)
                     /locus_tag="VGT44_18630"
     CDS             complement(1048..1932)
                     /locus_tag="VGT44_18630"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2582881.1"
                     /translation="MPELEVRQAREEDREAVLVFCRETWEWGDYIADVWDTWLHDENG
                     ALLVAVKEGKPVGIANLRMLNAQEAWMEGMRVDPAFRQQGVASALFDAQIAEARRRGA
                     STARLITNSTNTSAIRLIERTPMRRVGAYAIYETGQPATDSKRTYGLETPVLATPEDI
                     DEVIDYLNVSNIFPSVGGLYYQGFTAFTITAGLIEQKITAGQIYLLRRWQRLDGLVMC
                     ETREWREGRHLFIGYIDGTTESISLLAYALRAMLPQLGLEKAQAHIPDLMMVRDAFVG
                     AEYEWDGHVFYTYEWELI"
BASE COUNT          438 a          649 c          591 g          426 t
ORIGIN      
        1 cagtacgcgg cacgtgcgcg ccagcacgct tgcactgtgt cgcgccatgg cgggagccga
       61 cgcgcctggc ctgatcgatg gccccggcga tttcaaagat gaaacgttgg tcgacatggc
      121 ggtgatcgcc gcccggcgcg cgcttgccag tgcggggcgc gatgcctccg aaatcgacac
      181 ggtcatcggc gcgtcgtcat ccgataatga tgcgtttccc actatcgccg gccaggtgca
      241 gtaccgcctg ggcctgggtc ctatccgcgc taccatgctc aaaggggcgt gcgcctgcca
      301 gaccgaggca tttcagactt gcgccgagat acttgcggcc agttctgcga agctggtatt
      361 aatggtcacg gcagaggggt tgttgcccaa catcatgaat gtgttggact ggaaaacaag
      421 ttcgcttttt ggtgagggtg cgtccgctta tttgctcgaa atcggcgacg aagatactta
      481 cgtcattaat ggatcggacg cggcccaggc ccccgcgctc tattatcaaa ctcccctgcg
      541 caaggatgta ctggacatgg ccgaggttga tctgaaaatg cgccagcatt acgcggcggg
      601 caacggcaag gaattgaacg ctatcctcgc gcaataccat gtcggctata ccaaaatgaa
      661 cggcaaagag gtctttcgcg aggcccccag ggccatggcc gaggcaattg atgccctctg
      721 ccgccatgcc gggttgagtc acgacgaact cgcgcacatc atcccacacc aggccaattc
      781 gcgcatcacc cgccgtatgg gcgaactgtt gatccacgat tacggctggc catcatccac
      841 gatggagaag ctggtcgata atttccgcta ctacggcaac ctgtcgaacg ccagcatcgc
      901 gacagctatg gcggagctgc tgcgcaaaag caacctgcat gatggcgaat ggatggcgct
      961 gcccgccgtg ggtggcggca tgaactacgg ctgctggctg ctgcgctatc acggattggc
     1021 gcatcccgaa gccgtgatgg gtgacggtta aatcagctcc cactcatacg tgtagaacac
     1081 atgcccgtcc cattcgtact ccgccccgac aaaggcgtcg cggaccatca tcagatcggg
     1141 aatatgagcc tgtgccttct caaggccgag ctgcgggagc atcgcgcgca aagcataagc
     1201 tagcaggctg atcgattcgg tcgtgccgtc gatatagcca atgaagaggt gcctgccttc
     1261 gcgccactcg cgcgtttcac acattaccag cccatccagg cgttgccagc ggcgcaggag
     1321 atagatctgt cccgccgtga tcttttgctc gatcaacccg gcggtgatgg tgaatgctgt
     1381 gaagccctgg tagtagagtc cacctaccga tggaaaaata ttggagacgt tgaggtagtc
     1441 aatcacctcg tcgatatcct ctggcgtcgc cagcacaggc gtttcgaggc catacgtacg
     1501 ctttgagtcg gtcgcgggtt gtccggtctc gtagatggcg tatgcgccta cgcgccgcat
     1561 gggtgtgcgt tcgatcaagc gaatagcgga agtgttggtc gaattggtaa tcagccgcgc
     1621 cgtggacgcg ccgcgccgcc gcgcctcggc gatctgcgca tcgaacaacg cgctggccac
     1681 gccctgctgc cggaaagccg ggtcgacacg catcccctcc atccaggctt cctgcgcgtt
     1741 gagcatacgc aggttggcga tccccactgg tttcccctcc ttcacggcca ccagcagcgc
     1801 gccgttctcg tcatgcagcc aggtatccca gacatcagca atgtagtcac cccattccca
     1861 cgtctcgcgg caaaacacca gcaccgcctc ccggtcctcc tcgcgcgcct ggcgcacttc
     1921 caactctggc atcgaatctt actcctctgt atcgttcttc tctaaaagac tgtagcccga
     1981 aaagtcccat ccgtcaaccc ggtatagtaa accatccagt tcgattgccc ttacacgtag
     2041 ctggctaaac aagcagccga aacaatccag gaagatgatt cgtaacaata ctatccgtta
     2101 agat
//
LOCUS       DASZAK010001055         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_27256, whole genome shotgun sequence.
ACCESSION   DASZAK010001055 DASZAK010000000
VERSION     DASZAK010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27256"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VGT44_18635"
     CDS             complement(<1..862)
                     /locus_tag="VGT44_18635"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV2582882.1"
                     /translation="MHILPLRVALAQINVTVGDLEGNARKIRQAMERAADEGAHIICT
                     PELALTGYPPEDLLLKPDFVDANLRALDQLVEASRAFPGLTAIIGFVDRDHDSYNAAA
                     VIGEGRLYGKYHKHFLPNYGVFDENRYFQAGSTAPIFLVKDVHVGVNICEDIWYPTGP
                     MTTQAHAGAEVIININGSPYYASKGHFRQEMLATRAADNSVIVVYLNMVGGQDELVFD
                     GGSMVFNEAGTLIARAKEFEEDLLIVDLDTASVFRTRLHDPRRRQERLQLRDGEVPMI
                     TISGESSSAAK"
     gene            900..1460
                     /locus_tag="VGT44_18640"
     CDS             900..1460
                     /locus_tag="VGT44_18640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582883.1"
                     /translation="MDIQLYAIHVVPVILIVYFAFALFLHTPKEALLASLLGGLVMAI
                     LNALGDLLAYYASWWHYAASGLILQLPLPFYLTPLFIFGGLAYLLTWRFSKGREQAGE
                     GAKSSGANWPTLALLLGVPLLGFARDLLNAGITHSSFLVWDSPLAGPIDFVLWLVMFY
                     AGYTVFRRVLASRGLVQTMPGEKRAG"
     gene            complement(1557..>2460)
                     /locus_tag="VGT44_18645"
     CDS             complement(1557..>2460)
                     /locus_tag="VGT44_18645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582884.1"
                     /translation="ETTWATPPMRLTTSRDQDIELVASVTYQLQPEDAYIAVMQIADW
                     ESDLHQHFLAVLKSYAYELSPDDFIAWTHHVHSRPHIIDELASPMAETRWDRLNAAIM
                     RRLQDQVATRGIKINMVHILDITVISRGSHSIASTRSTPVDTGIARGQSPSMPPLSAR
                     QTEIAAQPTAQATPMVLPSQAPTPPLNLTPSTIQFMKDTFDSIRDGRITDLTIITKLL
                     HEFEQIANTPELDAQFPYDARKAASTLSYRLNYIKASQDVAKAQRASIPASPIPAPTV
                     PSEQSVRPARRLPTLPNDNLAGGG"
BASE COUNT          452 a          623 c          811 g          574 t
ORIGIN      
        1 tttttgccgc ggaagatgat tccccggaga tcgtaatcat gggaacctcg ccatcgcgca
       61 gctgtaagcg ctcctggcgg cggcgcgggt cgtgaagccg cgtgcggaag actgaggcgg
      121 tgtccaggtc aacgatgagc aaatcttcct caaactcctt ggcgcgcgcg atcaatgtgc
      181 ctgcttcgtt gaagaccata ctgccgccat cgaagaccag ttcgtcctgg ccgccgacca
      241 tgttgaggta gacgacgatg acgctgttat ctgctgcgcg cgttgccagc atctcctggc
      301 gaaaatggcc tttgctggcg tagtagggcg agccattgat gttgatgatg acttcggctc
      361 ccgcgtgggc ttgcgtcgtc atagggccgg tcggatacca gatatcctcg cagatgttga
      421 cgccaacatg tacatccttg acaaggaaga tgggggctgt gctgccggcc tggaagtaac
      481 gattctcgtc gaagacgcca tagttgggca ggaagtgttt atggtacttg ccgtagagcc
      541 tgccctcgcc gatgactgct gccgcgttgt agctatcgtg atcgcgatca acgaagccga
      601 tgatagcagt caggccgggg aaggcgcggc tggcttcgac cagttgatct agcgcgcgca
      661 ggttggcatc gacaaagtcg ggtttgagca gtaaatcctc gggaggatag cctgtgaggg
      721 ccagttcggg tgtgcagatg atatgcgctc cctcgtcggc tgcgcgttcc atcgcctggc
      781 gaatcttgcg ggcattgcct tcaagatcgc caaccgtcac attgatctgt gcaagcgcga
      841 ctcgaagcgg tagaatatgc ataggtacct ccggggtgcc gaatcagaaa ggtgaaacga
      901 tggacataca attatatgcg atacatgttg tgcctgttat tttgattgtg tatttcgcct
      961 ttgcgctgtt cctgcatacg ccgaaagagg ctctgctggc ctcgctgctc ggcgggctgg
     1021 tcatggcgat cctcaatgcg ctaggcgacc tgctagctta ttatgcctca tggtggcact
     1081 atgctgccag tgggctgatc ttgcaattgc ccctgccatt ctacctgacg ccgctgttca
     1141 tcttcggcgg gctggcctat ctgctgacct ggcgtttctc gaaggggcga gagcaggcgg
     1201 gtgaaggcgc aaaaagcagc ggcgccaact ggccgacgct ggcgctgctg cttggcgttc
     1261 ccctgctggg cttcgcgcgc gatctgctca acgcggggat cacgcattcc agcttcctcg
     1321 tgtgggatag cccgctggcc gggccaatcg attttgtgct gtggctcgtc atgttctacg
     1381 caggatatac tgtcttcagg cgtgtacttg ccagtagggg tcttgtacaa actatgccgg
     1441 gggagaagcg ggccgggtaa tcagaagatg ctttcgtatg aactgagtca ttgctgataa
     1501 agcgcggagt acgccttaaa ccaggctggg ccatgggggt atgattcttg cagctattac
     1561 cccccacctg caaggttatc attaggcagg gtgggcaggc ggcgcgccgg gcgcacggat
     1621 tgttctgatg gcacggtggg cgctggaatg ggactggcgg gaatgctggc gcgttgagcc
     1681 tttgccacgt cttgcgatgc tttgatgtag ttgaggcggt agctcagcgt gctggcggct
     1741 ttcctggcgt cgtaggggaa ctgcgcgtcg agttcagggg tgttggcaat ctgctcgaac
     1801 tcgtgcagca gtttggtgat gatggtcagg tcggtaatgc gcccgtcgcg gatcgaatcg
     1861 aaggtgtctt tcatgaactg aatcgtggat ggtgtcaggt tgagcggcgg cgtgggtgcc
     1921 tgggagggca gcaccatagg cgttgcttgc gcggtgggct gcgcagctat ttcggtctgc
     1981 cgcgcggata aaggtggcat actgggcgac tggccgcgag cgatacctgt atcgaccggt
     2041 gtggagcgcg ttgatgcaat ggagtgcgaa ccgcgcgaga tcacggtgat atccaggata
     2101 tgcaccatgt tgatcttgat gccgcgcgtc gccacctgat cttgtaagcg ccgcatgatg
     2161 gctgcgttga gacggtccca gcgcgtttca gccatagggc tcgccagttc gtcgatgata
     2221 tgcggacggc tgtggacatg gtgcgtccag gcaataaaat cgtcgggcga gagttcatat
     2281 gcgtagcttt tcaacactgc caggaaatgc tggtgtaagt cgctctccca gtccgcgatt
     2341 tgcataacgg cgatgtaggc gtcttccggc tgcaactggt aggtgacgga ggcgacaagt
     2401 tcgatatcct gatcgcggga ggtggtgaga cgcatagggg gagtggccca ggtggtttcc
//
LOCUS       DASZAK010001056         1932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_169113, whole genome shotgun sequence.
ACCESSION   DASZAK010001056 DASZAK010000000
VERSION     DASZAK010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169113"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="VGT44_18650"
     CDS             complement(<1..872)
                     /locus_tag="VGT44_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2582885.1"
                     /translation="MSTTEQSAVQAARAKVLAQPREGGTFLRSFNNSLVTILVVVIAV
                     LWSIPTFGLFVSSFRTPQAIASSGWWSGLLPPWNFTIQNYANVIQSSGMGQAFLNSLI
                     IVVPATIFPILFGAFAAFAFAWMRFPFRNTLFFGVVAMLVIPNQIALAPVLYWFTQLG
                     LTGQYASLWIAHTAFGLPFAVFLLRNFFAALPRDLIESAYIDGASNFRTFWTIIIPLS
                     VPALASLAIFQFMWVWNDLLIALIFVGSNQAVAPMTVVVANLVGSYGENWQLLTAAAF
                     LSMALPLIVFFALQR"
     gene            complement(869..1789)
                     /locus_tag="VGT44_18655"
     CDS             complement(869..1789)
                     /locus_tag="VGT44_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2582886.1"
                     /translation="MATVARERVVATAPPAKGREGISWTALIWIAPTVLLILAFFIYP
                     LFQTLVFSFQNADSTKFVGLKNYQFVFTDPDMLESLRNNLLWLVLATILTVGLGLIVA
                     VLVDRVKIEAIIKSALFIPMALSFVAASVIWKFVYDYAPPGSTQLGLLNAFLGLFGGQ
                     PQTWLQNPAIANFALIAIYTWMWTGFSMVIISAALKGIPDDVIEAAKMDGANRVTMFW
                     RIMVPMIAPTLGVVTTTMLINILKIFDVIYVETGGNYGTNVVAYQFYNQLFTFGNYGR
                     GSALAILLTVCVIPVMIINVRRMRREERGR"
BASE COUNT          500 a          508 c          555 g          369 t
ORIGIN      
        1 cgctgcaagg caaagaacac aatcagcggc agagccatcg agaggaacgc cgcggcggtc
       61 agcagctgcc agttttcgcc ataggaaccg accaggttgg ccaccacaac agtcatcggc
      121 gcgaccgcct ggttgctacc cacaaagatc agggcgatca gcaggtcgtt ccagacccac
      181 atgaactgga agatcgccag cgatgccagg gcaggcaccg acaacgggat aatgatggtc
      241 cagaaggtac ggaagttcga agccccatcg atataggccg attcgatcag gtcacgtggc
      301 agcgcggcaa aaaagttgcg cagcaggaaa accgcgaacg gcaggccaaa ggcggtgtga
      361 gcgatccaca gcgaggcata ctggccggtc aatccaagct gtgtgaacca gtagagaacc
      421 ggcgccaggg caatctggtt cggaatgact agcatcgcga cgacgccaaa gaagagcgta
      481 ttgcgaaaag ggaatctcat ccaggcgaaa gcgaaggcag cgaatgcgcc gaacaggatc
      541 gggaagattg tcgccgggac gacgatgatc aggctattga gaaatgcctg gcccatgcca
      601 cttgactgga tcacattcgc gtagttctga atagtgaaat tccagggcgg gagcagccct
      661 gaccaccagc cagatgacgc gattgcctgg ggcgtgcgaa acgaactgac aaacaggcca
      721 aaggtgggta tcgaccagag tacggcgata accacgacaa ggattgttac caggctatta
      781 ttgaaggagc gcaggaacgt accaccctcc cgtggctgtg ccaggacttt cgcgcgcgct
      841 gcctgtacgg cgctttgctc tgtcgtgctc atcgtccgcg ctcctctctg cgcattctgc
      901 gcacattaat aatcatcacc ggtatcacac acacggtcag gaggattgcc agcgcgctgc
      961 ctctgccgta gttaccgaag gtgaacaact ggttgtaaaa ctggtaagcc accacgttgg
     1021 tgccgtaatt gcctccggtc tcgacgtaaa tgacatcgaa gatcttgagg atgttaatga
     1081 gcatggtggt agtgacgacg cccagggttg gagcgatcat gggcaccatg atgcgccaga
     1141 acatggtaac gcggttggct ccatccattt tcgccgcctc gatcacatcg tccgggatgc
     1201 ctttgagcgc tgctgagata atcaccatgc taaagccggt ccacatccag gtatagatcg
     1261 caatcaacgc gaagttagcg atggccggat tctggagcca ggtctggggt tgccctccga
     1321 acaggcccag gaaggcgttg agcaggccga gttgggttga accgggcggc gcgtagtcgt
     1381 agacgaattt ccagatgacg ctggcggcca caaatgaaag cgccattggg atgaagagcg
     1441 cggatttgat gatggcctcg atctttaccc ggtctaccag aaccgcaacg atcaggccca
     1501 ggccgactgt caggattgtt gccagcacca gccagagcag gttattcctc agcgattcga
     1561 gcatgtccgg gtcggtgaag acaaactggt aattttttag cccgacgaac ttcgtggaat
     1621 cagcattttg aaagctgaag acaagggttt gaaacagcgg gtagatgaaa aaggccagga
     1681 tgagtaatac ggttggcgcg atccatatca aagccgtcca ggaaatacct tccctccctt
     1741 tcgcaggggg agcagtagca acaacgcgtt ctctagctac cgttgccata catgcctcct
     1801 ttgtcaaaat agtaatgtac gagtaccaca gtgcattcag gataccagga agacaacacc
     1861 tcgatcacga ggaaggtgaa ggatatgaac tgtgtcaatt ataccattac ggatatttct
     1921 tccacctcct ct
//
LOCUS       DASZAK010001057         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_383558, whole genome shotgun sequence.
ACCESSION   DASZAK010001057 DASZAK010000000
VERSION     DASZAK010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383558"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..993
                     /locus_tag="VGT44_18660"
     CDS             139..993
                     /locus_tag="VGT44_18660"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2582887.1"
                     /translation="MSNRLKFGLTGSNGGVTSHPEELVRLAQTAEKAGLDSLWAGEHH
                     IIATSSTIIPATSRFLNPVVALTYVAALTHTLRLGTGILLLPQHQPLILAKELASLDV
                     LSGGRLIVGIGVGWAEAEYEALGVPYHERGARADEYLAAMRAIWSEETPAYHGRYVSF
                     QDVQAFPHPVQQPTPPIVIGGRAPAVLRRTVEQANGWFGYALNLDETAAMLAQLREAA
                     SRYPRPASLGEIEISVAPRIPLDKDTAQRFADLGVHRLIMIPPRGLDAPSLEQWVTTI
                     GETLVDRV"
     gene            complement(1036..2025)
                     /locus_tag="VGT44_18665"
     CDS             complement(1036..2025)
                     /locus_tag="VGT44_18665"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2582888.1"
                     /translation="MMSAEMSAAVLRKHATPPEYDLQWPIPQRGSGQALVRVLAVPIS
                     PLDLLCASGTSYFGAPKLPYIPGTQGVGIVMEADVLASGQRVWFSCDAGMKPGDGSMA
                     QYCLIEEASALALPAEIESDLAAALGLSAIAAWMALTWRGRLQPGEQVLVLGASGAVG
                     QVAVQAARLLGAGRVIAASRDKDGLALALSRGADAAVDLSGDDVDEVSRRIAAACDGP
                     LQLVIDPVWGLPAEAAGRVLATGGRFVNIGSAAGASARFESAIVRSRLHNILGYTNNA
                     LTHEQKAQALSQILTHAAAGRCTVEREMVPLAQAGSAWERQAASARRKLILVP"
BASE COUNT          411 a          734 c          656 g          407 t
ORIGIN      
        1 cctggcgcac ccttgcagtc gccatgcctc gaacccaagt acagatttgc gcccccgata
       61 cgtttgtgac aaaataaagc ggaggcaaca tgaggtgtct caaccaccac actcaatcat
      121 tcccaatgga gataaagtat gtcaaatcgt ttgaaattcg ggttgaccgg cagcaacggg
      181 ggagtaacca gccatccaga ggagcttgtg cgtctcgcgc aaacagccga aaaggccggg
      241 cttgactcgc tgtgggcggg cgaacaccat atcatcgcca cgtcctcgac catcattccg
      301 gcgaccagcc gcttcctcaa cccggtcgtc gctctcacct acgtggcggc cctcacccat
      361 acactccgcc tcggaaccgg catcctgctg ctcccgcaac accagccgct catcctggcg
      421 aaggaactgg ccagcctgga tgtcctctcc ggcggcaggt tgatagtcgg gattggcgtc
      481 ggctgggcag aggcagagta cgaagcgctg ggcgttccct atcacgaacg cggcgcgcgc
      541 gcggatgagt acctggcagc catgcgcgcc atctggagtg aggaaacgcc ggcctatcac
      601 ggtcgctacg tctcgttcca ggacgtgcag gcgttccccc atcccgtcca gcagcctact
      661 ccccccatcg tcataggtgg tagagccccc gcagtcttac gccgcacagt tgagcaggcc
      721 aatggttggt tcggctacgc gctgaatctc gatgaaaccg ccgcgatgct cgcccaattg
      781 cgcgaagccg ccagccgcta ccctcgtccg gcctcgcttg gcgaaattga gattagcgtc
      841 gcccctcgca taccactcga taaggacacc gcgcagcgct ttgccgacct gggcgttcac
      901 cgcctcatca tgatcccgcc gcgcggcctg gatgcgccat ccctggaaca gtgggtaacg
      961 acgataggtg agacgctggt ggatcgggtt tgaaggagcg cagcaagtta gcctggtacc
     1021 gtcaggcttt actcatcatg gaacaaggat gagcttgcga cgagctgacg cggcctggcg
     1081 ctcccaggcc gagccggcct gtgccagcgg caccatttct ctctcgaccg tgcagcggcc
     1141 tgcagcggcg tgtgtcagga tttgcgagag tgcctgggct ttctgctcgt gggtcagcgc
     1201 attgttggta tagccgagga tgttgtgcag cctgctgcgc acgatggccg actcaaaacg
     1261 cgccgaggct cctgcggcag agccgatatt gacgaacctt cccccggtgg ccagcacgcg
     1321 accggcggcc tccgccggga gaccccaaac agggtcgata acgagctgca aaggaccgtc
     1381 acaggctgcg gcgatgcgcc tgctgacctc gtccacgtcg tccccagaga ggtcaacggc
     1441 ggcatcagcg ccgcgtgaga gcgccagggc gagcccgtcc ttgtcacgcg aggcggcaat
     1501 cacgcggcca gctccaagca gcctggccgc ctgtactgcc acctgtccga ccgcgccgct
     1561 ggctcccagg accagcacct gctcaccagg ctggaggcga ccgcgccagg tgagcgccat
     1621 ccaggcggca atcgcggata gtccgagcgc cgcggcgaga tcgctctcga tctcggcggg
     1681 aagagcgagc gccgacgcct cctcgatcag gcaatactgc gccatgctgc cgtccccagg
     1741 cttcatgccg gcatcacaac tgaaccacac gcgctgtccg gacgctagga catcggcctc
     1801 catgacgatg ccgacaccct gcgtacccgg aatataggga agctttggcg ccccaaagta
     1861 ggacgtgccg gacgcgcaca gcagatccag cgggctgatg gggaccgcca ggacccgcac
     1921 cagcgcctgc ccgctccctc gttgaggaat gggccattgg agatcatatt cgggtggagt
     1981 ggcgtgtttg cggagtactg ccgccgacat ctccgcgctc atcatggcat cctttcattc
     2041 gctgccagtc ttactatcgc tgtgcgcatc tgttcgtaaa gtacgctatt actcctgcgg
     2101 ggatgttcgt caagcttccg cgctcttctg ttgtgctgcc ttcccatgtt cccggtcggt
     2161 gtaggtatac gggttctcaa tcaattcgac cgctgccttc tcggggtt
//
LOCUS       DASZAK010001058         3861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_132353, whole genome shotgun sequence.
ACCESSION   DASZAK010001058 DASZAK010000000
VERSION     DASZAK010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132353"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VGT44_18670"
     CDS             <1..788
                     /locus_tag="VGT44_18670"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2582889.1"
                     /translation="CVNVTYEAPPTFDMVAMSRLIDAAVATKPDGLVVSIPDANALGP
                     SIKAAVAAGIPVISINSGSDVAQSLGVLVHIGQTEEVAGMGGGQKMGAAGVKHALCVN
                     QEVGNAALELRCKGFTEGLTAADGGTVKEIGVNLNNPTQTKQTIEAAIQHDPTIDGIL
                     TLGPTGATPALQALTDLNDVGKVKLATFDLSSDVLTAIKNGQMLFAIDQQQYLQGYLP
                     IVLLTLYKTNLNTIANQVLLTGPGYVTSQNVAQVIQLTSQGPR"
     gene            947..2008
                     /locus_tag="VGT44_18675"
     CDS             947..2008
                     /locus_tag="VGT44_18675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2582890.1"
                     /translation="MSTTTQGGSGAPASSERMLRSSPLRRIFIRPETGAVAGSVAVWI
                     FFAIVSGSTGFLSLLGTANYLTVAADLTIQAVPVALLMIGGEFDLSIGSTMGACGMII
                     AILSAQYGLNLWVAIVVALVFAALIGVLNGYLVVRTGLPSFIITLGTSYILLGLTTGL
                     TSALTGLTVVGGVDKTAGYNAAHAIFGSKIIIGGAGFPVDILWALIIVAVATWVLLGT
                     SFGNWIFGVGGDANAARNIGVPVRSVKILLFVGTAVLSCLLAIIQVVSFTSSQVLLGQ
                     GNEFLAIVSAVIGGVLLTGGYGSAIGAMFGALAFGIVQQGIVFAGVDINWYKAVVGVI
                     LLLAVLLNSYVRKLAMEKR"
     gene            2005..2931
                     /locus_tag="VGT44_18680"
     CDS             2005..2931
                     /locus_tag="VGT44_18680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2582891.1"
                     /translation="MMSMIDQPTAGRTGVPLLEVSHISKYFGSVIALKDISMKVYAGE
                     VMCLLGDNGAGKSTLIKILSGVHRPSEGDYMVEGKQANFSSPRDALSAGIATVFQDLA
                     MIPLMGISRNFFLGSEPTRGWGPFKNFDVKKADGITREELWKIGIQVRDTTQPVGTLS
                     GGERQSVAIARAVYFGAKVLILDEPTAALGVKEAGIVLRYIAQARARGLGVIFITHNP
                     HHAYPIGDHFTLLNRGRSMGTFAKSEITREGLTSMMAGGEELETLSHELAEFARTDAI
                     AGAKLAEAAQEFEAEAESMEDGHQGLPSTQSE"
     gene            2944..>3861
                     /locus_tag="VGT44_18685"
     CDS             2944..>3861
                     /locus_tag="VGT44_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2582892.1"
                     /translation="MIQDGHLFNYVGGKWKRSSASDFLDVRNPATGEAMVRVPLSSRA
                     EVDEAARAAQTAFADWRRTPPLERVQYLFKFKRLLEEHFDDIARLTTQECGKTLDEAR
                     GELQRGLENVEVATGIPSLMMGYNVEDIARGIDEHMLRQPVGVVAAITPFNFPGMIPL
                     WFLPYAIACGNCFILKPSEKVPMTMSRVFELVDQLGLPPGVLSLVNGAREAVEALLDH
                     PDVRGISFVGSSPVAKYIYRQASANGKRVQCQGGAKNVVVVLPDADLETATRIVADSS
                     FGCAGQRCLATSVAITVGEARSPFTEQITH"
BASE COUNT          749 a         1116 c         1174 g          822 t
ORIGIN      
        1 tgtgcgtcaa tgtaacctat gaagcgcctc cgaccttcga catggtggcg atgtcccgac
       61 tgatcgatgc ggcggtggcg acgaagccgg acgggctggt cgtctcgatc cccgatgcca
      121 acgcactcgg cccctcgatt aaagccgccg ttgccgccgg tattccggtg atctcgatca
      181 actccggctc cgacgttgcg cagagcctgg gggtgctggt ccacatcgga cagaccgagg
      241 aggtcgctgg tatgggtggt ggccagaaga tgggggctgc cggtgtcaaa catgctctgt
      301 gcgtcaacca ggaggttggt aacgccgcgc ttgaactgcg ttgcaagggc tttacggaag
      361 gtctgacagc ggcggacggc ggcaccgtga aggagatcgg cgtcaatctg aacaacccca
      421 cgcagaccaa gcagactatt gaagccgcga tccagcacga tcccactatt gacggcattc
      481 tgacgctcgg cccaacaggc gctacccctg ccttgcaggc gctgaccgac ctgaatgacg
      541 ttggcaaggt caagctggca accttcgacc tgtcatccga tgtgctgact gccattaaga
      601 acggccagat gttgttcgcc attgaccagc agcagtacct ccagggctat ctacccatcg
      661 tcctgctcac gctgtacaaa acgaacctca acaccatcgc taaccaggtg ctgttgaccg
      721 gccctggcta tgtcacatcg cagaacgtcg cccaagtcat tcagttgacg tcgcaaggac
      781 cgcgctaaac cgtcccccgg cagggggata tgaatggagg tgtaggacag cgttgtaggg
      841 gcgcaattca ttgcgccctc gcgccaggcc gatgcgtgga gaaaaccagg gcgcaatgaa
      901 ttgcgcccct acaacgcttt cctacaccct ctgaatggag ggaaagatga gtacaactac
      961 ccagggtggc tctggtgcgc ctgcttcaag cgagcgaatg ttacgtagca gcccgctgcg
     1021 tcgcatcttc attcgcccgg agaccggtgc agttgccggt tccgtagctg tctggatttt
     1081 cttcgctatc gtctcaggga gtaccggttt tctcagcttg ctcggcacag caaactatct
     1141 gaccgtcgcg gcagacctga cgattcaggc tgtcccggtc gcgttattaa tgatcggcgg
     1201 agagtttgat ctctcgatcg gttcgacaat gggtgcctgc ggcatgatta tcgctattct
     1261 cagcgctcag tatggtttaa acctctgggt cgcaattgtc gtcgccctgg tcttcgcagc
     1321 gctgattggc gtgttgaatg gctacctggt ggtacgcacc ggcttgccat ctttcatcat
     1381 tacacttggt acgtcctata ttctgcttgg cttgacgacg gggctgacct ccgctctcac
     1441 cggattgacc gtggtaggcg gtgtggacaa gacggctggc tacaatgccg ctcatgccat
     1501 attcgggagc aaaatcatta ttggcggcgc cgggttcccg gttgacattc tctgggcgtt
     1561 gattatcgtc gcggtcgcaa cctgggtact gctcggaacc tcgttcggta actggatctt
     1621 cggcgtcggc ggggatgcca atgccgcgcg caacatcggc gtaccggttc gcagcgtgaa
     1681 gatcctactg tttgtcggaa cggcagtgct ttcttgcttg ctggccatta tccaggttgt
     1741 gagcttcacc agttcgcaag ttctgcttgg tcaggggaac gaatttctgg caatcgtctc
     1801 ggcggttatc ggtggcgttt tgctcaccgg cggctatggc tcggccatcg gcgcgatgtt
     1861 tggcgcgctg gcctttggca ttgtacaaca gggcattgtc ttcgcgggtg tagatatcaa
     1921 ctggtacaaa gcagttgtgg gagtgatcct gttgctcgcg gtgctgctca acagctacgt
     1981 gcgcaaacta gcaatggaga agcgatgatg agtatgatcg atcaaccgac tgctggcagg
     2041 acaggcgttc cactgctaga agtgagtcat atctccaagt acttcggcag cgttatcgcc
     2101 ttgaaagaca tctccatgaa ggtctatgca ggggaagtaa tgtgcctgct gggagacaat
     2161 ggcgcgggta aatccaccct gattaaaatt ctttccggtg ttcaccgtcc cagcgagggt
     2221 gactacatgg tcgagggcaa gcaagccaac ttctcctctc cgcgcgatgc gctgagtgct
     2281 ggtatcgcga ccgtcttcca ggacctggcc atgatcccgc tcatgggtat ctcgcgcaac
     2341 ttctttcttg gctcagaacc aaccagaggc tggggaccat tcaaaaactt cgacgtgaaa
     2401 aaggctgatg gcatcacgcg cgaggaactg tggaagatcg gcatccaggt gcgcgataca
     2461 acgcagccgg tcggcacgct ctccggcggc gagcgccagt cggtcgccat tgcgcgcgcc
     2521 gtctactttg gcgctaaggt cctgatcctc gacgagccaa cggctgccct gggcgtcaaa
     2581 gaggcgggca tcgtcctgcg ctacatcgcc caggcgcgcg cgcgcggcct gggggtcatc
     2641 ttcattaccc acaacccgca ccacgcctat cccatcggcg atcacttcac gctgctcaat
     2701 cgcgggcgca gtatggggac cttcgccaag tcggagatca cgcgcgaggg attgacctcg
     2761 atgatggccg gtggcgagga actggagacc ttgagccacg aactggccga gttcgcgcga
     2821 accgacgcaa tcgccggcgc gaaattggcc gaggctgccc aggaatttga ggcagaagcg
     2881 gagtctatgg aagacgggca ccagggcttg ccatctactc aaagcgagtg aggaggtcta
     2941 tatatgattc aagatggaca tctgttcaat tatgtcggcg gaaagtggaa acgttcgagc
     3001 gcgagtgatt ttctggatgt gcgcaatccc gcgacgggcg aggcaatggt acgtgtgccg
     3061 ctctcgtcgc gcgctgaagt ggacgaagcg gcgcgcgcgg ctcagacggc cttcgctgat
     3121 tggcgtagga cgccgccgct cgagcgcgtc cagtatctct tcaagttcaa gcgcctgctg
     3181 gaagaacact tcgatgacat cgcgcggctg accacgcagg agtgtgggaa aacgctggac
     3241 gaagcgcgcg gcgaactgca gcgcggcctg gagaacgtcg aagtcgcaac gggcattcca
     3301 tctctgatga tgggctacaa cgtcgaagat atcgcgcgcg gcatcgacga gcacatgctg
     3361 cgccagccgg tcggggtcgt cgccgctatc acgcccttca actttcccgg catgatcccg
     3421 ctctggttct tgccctatgc gattgcctgc ggcaactgct tcattctcaa accatcggaa
     3481 aaggtgccga tgacgatgag ccgcgtcttc gagctggtcg atcaactcgg tctgcccccc
     3541 ggcgtcctca gcctggtcaa cggggcgcgc gaggctgtgg aggcgctgct cgatcacccc
     3601 gacgtgcggg gcatctcctt cgttggctct tcgccggtcg cgaagtacat ctaccgccag
     3661 gcatcggcca atggcaagcg cgtgcagtgc cagggcggcg cgaaaaacgt tgtcgttgtc
     3721 ctgccggatg ctgatctaga gacggcgacg cgcattgtgg ccgactcgtc ctttgggtgc
     3781 gcgggccagc gctgcctggc aacctctgtg gctatcaccg ttggtgaggc gcgctcgccc
     3841 tttacggagc agatcaccca t
//
LOCUS       DASZAK010001059        10084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_383574, whole genome shotgun sequence.
ACCESSION   DASZAK010001059 DASZAK010000000
VERSION     DASZAK010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 10084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10084
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383574"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VGT44_18690"
     CDS             complement(<1..461)
                     /locus_tag="VGT44_18690"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2582893.1"
                     /translation="MDVVNSSNGLVTLQFLTGPLAGRAIPINKPIVTIGREPTNDISV
                     PDPQVSRQHARLVYNSGQWLIEKIPAQNTISVNSRDVQQSPIANRDIIALGTGTTFVF
                     LTSPDIAGAGQASLPPAQRYVPPPYGGPQSTPQQLQQPGQPAAPAQQPFPQP"
     gene            complement(561..2150)
                     /locus_tag="VGT44_18695"
     CDS             complement(561..2150)
                     /locus_tag="VGT44_18695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582894.1"
                     /translation="MSDEYDTTRDDWDESPEEDSSSRQSRPSRQPRQRLPQDNTQRPG
                     RNLPPGRRFTPARNTGRYQNQRPPQQSQPSSRSTSPGQQRSPNRQQEPRDSQVEDNEY
                     EQFQRYRRYQQAQASRQQARRPRTEDEEYEQFQRYQQYRQSSQRATRQSRGDTSEQTR
                     RSPGQPMYVHDDQEDTYQRIPPRRVTRPIPADLPVTTSTRPVRPRRRVWSTLFIGCVS
                     AILTIAVLAAIIVFVVFRVLPNTVPGFNLGATGYHDKQQTLSLPITTNISQLAIHNPA
                     GNITITTDANATTGTLTYKKNTLATSQGNASAEFARITVTVKPGSGSDTTIPSGCPAT
                     TCLEVTTTIPNASNDAVDLQLTLPPQNPSPIFMLSATTQTGNLSVQNVKGLLSLTDDT
                     GTMSVMGGLLASGSCLQDRIGNITFAETLETTVPPTINPCTGSPVNPSGASSQQPWYS
                     IKTGSGNVDATFNTVSTNIQLDASIRNQGKINSDFPIAITQNPDGTSSYFGPLLPNTQ
                     PTALLTLTVDVAGNITLHKAS"
     gene            complement(2147..3910)
                     /locus_tag="VGT44_18700"
     CDS             complement(2147..3910)
                     /locus_tag="VGT44_18700"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582895.1"
                     /translation="MQHDKETRYSNRMALLAMLAACCAVFLTAIDQTVVVTALPRIIT
                     DLSINVQQLDRAAWIVSGYLLGYIIAMPLMGRISDMYGRRSVFLLCLGIFAAGSFLCG
                     RSLDLGNSVDISFLQHIGLNVASPDNIGAPGTLSPGLVWLVAARFIQAIGGGALIPVA
                     MAVAGDFYGEKQRSLALGLIGMVMETGGVLGPLYGALIVQTLGWQAIFYLNLPIVAVL
                     AALTWFYIPSRKQPLAATDALPIRITRSRVDIAGALLLGASLACLSLGLSQEAATATT
                     GISANPNAAPIQNNPWLIGASLLLLAVFLLREWWLARRETRPGKKIIGSEDGLNRVPT
                     TRQVLPIVELSVFRRAAFSAAALVSLLVGSALIIAMVDIPFFIDTVLGGSALDSGLAL
                     LRLTAMIPVGALLGGYLCPRITCRITAALGLLLSALGFFLMHLWPLDVNWLQITASTL
                     IGGFGFGLVIAPIGTTAINAVRSTQMGMASSTVTVLRMTGMIIGLAALTSWGLGRFHT
                     LMLAFQPPPGAKPFDPAYNAALTHSATAAGHDVYTSIFLAAGIITLVAVLPALLLEGK
                     QASPYALSRSKGFTALKRGLE"
     gene            complement(4011..4724)
                     /locus_tag="VGT44_18705"
     CDS             complement(4011..4724)
                     /locus_tag="VGT44_18705"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEV2582896.1"
                     /translation="MQTRQRTSQTKIPIMLALLLALVLLLAACDNNSGNTTPSAQTLI
                     KSAQQAIQKVSAYHFNLQAQNIGTSGKFPITSADGDIVVPDRLKATATISGNAVQIVA
                     IGTRQYINLFGGWQTVSGLIDPRTLSDPKTGVSAILGHIQNPSQPTDSSVNGTNCWLI
                     KGKLDPVLLAGITGGGAPTGTRDDVSVCIGKADNLPYQIVITGPAAQGDTSQTSRTIT
                     LSKFNETITINPPTGTSIV"
     gene            4884..5546
                     /locus_tag="VGT44_18710"
     CDS             4884..5546
                     /locus_tag="VGT44_18710"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEV2582897.1"
                     /translation="MQKGLEHIMEQKQVNVPLRVRLRLSAERTPGEEGRVPAWVEQMG
                     KAALEAAEVTSVGQQQVDRANEGMRQVREATLQSARAIKRLGEGGQEINNTVVELTDL
                     TTRMNLLALNAAIEAVRAGEQGQGFAVIAQEIRALSAHCAEAARKVSARLHAIQSEVA
                     VASKSVDASITQVVVQCELVTQMGVALDAIGIVTEQMATLAQGLGATSPAYRLEDSSS
                     LY"
     gene            complement(5500..5874)
                     /locus_tag="VGT44_18715"
     CDS             complement(5500..5874)
                     /locus_tag="VGT44_18715"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2582898.1"
                     /translation="MKEHVWVIDDQQDILDVVQIVLDDAGYQTSTYLTGASLLQPMEH
                     PPDLILLDILLSGEDGRVICRQLKSREQTRQIPVVLLSAHFNAAKAIEECGADDFLAK
                     PFHLDELVNTVKKNLPDDTPEK"
     gene            complement(6311..6700)
                     /locus_tag="VGT44_18720"
     CDS             complement(6311..6700)
                     /locus_tag="VGT44_18720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582899.1"
                     /translation="MADSDPIASSPSPQSETQLPPEAQGETNGGPLGCCLGVVVGLLL
                     SLSIAILSRLYATPLFDALHGWLSFTVRAVMIIVGLLASIVFGYLGWRIGARIYREYE
                     PSPRQKQRLERLQQKQRRKQRARRKYI"
     gene            complement(6700..7413)
                     /gene="upp"
                     /locus_tag="VGT44_18725"
     CDS             complement(6700..7413)
                     /gene="upp"
                     /locus_tag="VGT44_18725"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV2582900.1"
                     /translation="MAETNTSSGGRPNPPMRGVPQPSREEVMQLPRVHISSHPVMKHK
                     MTALRDKNTPPPEFYNLVKEIGALLAYEATSNLTLEPRQIETPMSPMIGERLAGGIGV
                     TPILRAGLGLAEGFRQVIPDVQVWHLGLRRDEHTLEAMEYYNQLPHKVDLQVCYAVDP
                     MLATGGSAIDAINVLKRRNIPRLCYVGIIAAPYGLLKLSQTHPDIDIYIAALDESLND
                     IGYIVPGLGDAGDRQFGTF"
     gene            7551..7841
                     /locus_tag="VGT44_18730"
     CDS             7551..7841
                     /locus_tag="VGT44_18730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEV2582901.1"
                     /translation="MIVGVCRITLHLPGCHSLKEKRQVVKSLMARVRNRFEVAIAEVE
                     EQDRWQTAVLGVTCVSNSSQHADEVLGHVQRFIEETRPDILVTNNEMEIMHW"
     gene            7871..8665
                     /locus_tag="VGT44_18735"
     CDS             7871..8665
                     /locus_tag="VGT44_18735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HEV2582902.1"
                     /translation="MSTELEPHRWRSSRVLGVRVDAVTREQALDAIEQFAAKRRASLE
                     TGDVLACQQVVTVNTEFVMEAQRNSAFREAINAAALVVADGVGIVWATRLRGTPVPER
                     ITGTDTVMDVAKRCARSGYRLYLLGAAPGVAELAAERLQAIAPGLEIAGTYAGSPAPE
                     EEDGIVERLNAARADVLCVAYGAPAQELWIRRNLERLPVAVAMGVGGAFDFLSGRQRR
                     APRIMQRLGLEWLYRLYREPWRWRRMLAIPQFLLYVLLKIRGDKSS"
     gene            8995..10014
                     /locus_tag="VGT44_18740"
     CDS             8995..10014
                     /locus_tag="VGT44_18740"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HEV2582903.1"
                     /translation="MKEELHIANNPLQAIIRGFAGKRVLVIGDMVADEYLVGRPARIS
                     REAPVLILELYEERTVPGGATNVAVNARSLEAEVFLAGVVGDDVPGQRLRRAVDSLGM
                     HQEGLVTHPTRPTSTKTRIMAGSSQIVQQHIVRIDRVDTSDLDDGSQAAIIEYVRAVL
                     PSMDAVVLSDYENGVISPKILEVCLPMARELDKVIVADSHELLLRFQGVTAITPNQPE
                     AERTLGVTINSRAELDEAGQRLLAGSAAQSILITRGAEGMSLFEVGKPPLHLPVYRLD
                     HASEIVDTNGAGDTVAATFTLALTAGASFAEAAYLANAAAALVVRRLGCASNTPQELM
                     SALHE"
BASE COUNT         1957 a         2490 c         3496 g         2141 t
ORIGIN      
        1 ggttgcggga acggttgttg ggctggagca gcgggctgcc cgggttgttg caactgctgt
       61 ggcgtcgatt gtggtccgcc atagggaggc ggaacgtacc gctgcgccgg tggaaggctg
      121 gcctgccccg cgcccgctat atcgggtgag gtcaaaaata cgaaggtggt gccggtacca
      181 agggcgatga tgtcgcgatt ggcgatcggg ctttgttgca catcgcgcga attgacggag
      241 atcgtgttct gcgccggaat tttctcgatg agccactgtc cgctattgta gaccaaccgg
      301 gcgtgttgac gcgagacctg tgggtctgga acggagatat cgttggttgg ttcgcgtccg
      361 attgtcacga tgggcttatt gatcggtatg gcgcgtcccg ccagtgggcc ggtaaggaat
      421 tggagcgtca ccaatccgtt tgagctgttc accacgtcca tatgctcgct ccttgctgga
      481 atgatttatg gcaacccgct gtgtgtaagg ccattatact gtagcgattg tctgctggca
      541 atatgggaaa tttgagttcc ctacgaggct ttatgcagcg tgatattccc ggcgacatcg
      601 acggtcaggg tcaggagggc tgttggctgg gtattgggaa gcagcgggcc aaaatagctg
      661 gaggtgccgt ctggattctg ggtaatggca atcgggaaat cgctgttgat tttgccctgg
      721 ttgcggatgg aggcatcgag ctggatattc gttgaaactg tattgaacgt ggcatcgacg
      781 ttgccggaac ctgtcttgat gctgtaccag ggctgctggc tcgacgcgcc tgatgggttg
      841 acaggtgagc cggtgcaggg attgatggtc ggaggtacag tggtttcgag cgtttcggca
      901 aacgtgatgt tgccaatgcg gtcctggagg cacgaaccgg aggcaagcag gccgcccatg
      961 acgctcatgg tgccggtgtc gtcggtgagc gagagcagac ccttgacatt ctgcacagag
     1021 agattgccgg tttgcgttgt ggcgctgagc atgaagatag gagatggatt ctgcggcggc
     1081 agtgttaact gtagatcgac cgcgtcgttg ctggcgttag ggatggttgt tgtgacctcg
     1141 aggcaggtgg ttgccggaca gccggaaggg atggttgtat ccgagccgct gccgggtttg
     1201 acagtaacgg tgatgcgcgc gaattcggcg gaggcgttac cctggctggt ggccagggta
     1261 ttttttttgt aggtcagcgt gccggtggtg gcattcgcat cggtcgtaat ggtgatattg
     1321 ccagcagggt tgtggatggc gagctgggag atattggtgg taatgggcag cgagagggtc
     1381 tgctgcttgt cgtggtaacc ggtcgcgccg aggttgaaac cgggaacggt attgggcagg
     1441 acgcggaata cgacgaagac gataatggcg gcgaggacag cgatagtgag gatagcgcta
     1501 acgcagccga tgaagagcgt tgaccagacg cgacggcgcg ggcgaaccgg tctcgtcgag
     1561 gtggtcacag ggaggtcagc aggaattggc ctggtgacac ggcgcggcgg aatgcgctgg
     1621 taggtatctt cctggtcgtc gtgaacgtac atgggctggc caggggagcg cctggtttgc
     1681 tcagatgtgt ctccgcggga ctggcgtgtt gcgcgctggg aggattggcg gtattgctgg
     1741 tagcgttgga actgctcgta ttcttcatcc tcagtgcgag gtctgcgcgc ttgctgccgg
     1801 gatgcctgag cctgctggta gcgcctgtag cgctggaatt gctcatactc gttatcttcc
     1861 acctgggaat cgcggggttc ctgctggcga ttgggcgaac gctgctgtcc tggcgacgtt
     1921 gatctgctcg acggttgcga ctgttgtggc gggcgctggt tctggtagcg gccagtattg
     1981 cgggcagggg tgaatcgacg acctggggga aggttcctgc ccggccgctg tgtattatcc
     2041 tggggcaaac gttgacgggg ctggcgcgaa ggtcgggact gccttgaaga ggaatcctct
     2101 tcgggtgact catcccaatc gtctcgcgtg gtatcatact catcgctcat tctaagcctc
     2161 ttttcagcgc cgtgaaacct ttgctccttg atagggcata gggtgatgcc tgtttgccct
     2221 ccaacagcag agcaggtagt accgctacaa gcgtaattat accagcagcg aggaagatcg
     2281 aggtgtagac atcgtgaccc gccgcagtag cagagtgtgt gagggcggcg ttgtaggctg
     2341 gatcgaatgg ctttgctcca gggggcggct ggaaggcgag catgagggta tggaagcgac
     2401 ccaggcccca cgaggtcagc gcggcaaggc cgataatcat gccggtcatg cgcagcacgg
     2461 tgacggttga agaggccatg cccatctgcg ttgatcgcac agcgttgatg gcggtggtgc
     2521 cgatgggagc gatgacaaga ccgaagccaa agccgccgat cagcgtgctg gcggtgatct
     2581 gaagccagtt aacatccagg ggccacaggt gcatcaggaa gaagccgagc gcgctgagca
     2641 gcaggcccag cgccgccgta atgcggcagg tgatgcgggg acacaggtag ccgcccagca
     2701 gcgcgccaac ggggatcata gcggtcaggc gcagcagtgc cagcccgcta tccagcgcgc
     2761 tgccgcccag caccgtatcg atgaagaagg ggatatcgac catcgcgata atcaaggcgc
     2821 tgcctaccag caggctgacc agcgcggctg ccgaaaacgc cgcgcgccgg aagaccgata
     2881 actccacgat aggcaacacc tgtcgcgtcg ttgggacgcg attgagcccg tcctcgctcc
     2941 cgataatttt tttaccgggg cgggtttccc tgcgggcgag ccaccattct cgcaggagga
     3001 acactgccag caacagcagc gaagcgccga tcagccaggg gttgttctgg ataggggcgg
     3061 cgttcgggtt ggcgctgatg ccggtggtgg cggtcgcggc ttcttgcgac aggcccaggc
     3121 tgaggcaggc cagcgacgcg cccagcagga gcgcgccggc gatatcgacg cgacttcggg
     3181 taatgcggat aggaagagca tcggttgctg ccagaggttg cttgcgagag ggaatgtaga
     3241 accaggtcag cgcggcaagg acggccacga tgggcaggtt gaggtagaag atggcctgcc
     3301 agcccagcgt ttgcacgatg agcgcgccgt acaacggccc cagcacgccg ccggtttcca
     3361 ttaccatacc gatcaggccc agcgccaggc tgcgctgctt ctcgccgtaa aagtcgcccg
     3421 cgacggccat ggcgacggga atgagcgcgc cgccaccgat ggcctggatg aagcgcgccg
     3481 cgaccagcca gaccaggcca ggtgagagcg tgcctggcgc gccgatgttg tcaggcgagg
     3541 cgacattcag gccgatatgt tggagaaagc tgatatcaac cgagttgccc aggtccagcg
     3601 agcgaccaca gaggaacgac ccggcggcga agatgccgag gcagagcaag aagacactgc
     3661 gccgcccgta catgtcggag atacgcccca tgagcggcat ggcgatgatg tagccgagca
     3721 ggtagccact gacgatccag gcggcgcggt caagctgctg cacgttgatc gacaggtcgg
     3781 tgatgatgcg cggcagcgct gtcacaacga ccgtctggtc gatggcggtg aggaaaaccg
     3841 cgcagcaggc cgccagcatt gccagcagcg ccatgcgatt actatatcgg gtctctttgt
     3901 catgctgcat acttattcac tccgaaatgt gtagggacag ggggagcggc ggagagcggc
     3961 gggggccttg cgcctgtccc tacagatagt gagatagata tacataggta ttacacgatt
     4021 gatgtgccgg ttggcgggtt gatggtgatg gtttcgttga atttcgagag cgtgatggtg
     4081 cgtgatgtct gggaagtgtc gccctgggct gcggggccag taatcacgat ctggtagggc
     4141 aggttatcgg ctttgccgat gcagaccgag acatcatcgc gtgtgcctgt gggtgcgccg
     4201 ccgccggtga tgcctgccag cagtaccggg tcgagcttgc ctttgatgag ccagcaattg
     4261 gtgccgttga cgctggaatc ggtcggctgg ctgggattct ggatgtggcc aagtatggcg
     4321 gagacgccag tcttcgggtc ggagagcgtg cgcgggtcga tcaggccgct gacggtttgc
     4381 cagccgccga agaggttgat gtattgcctg gtgccgatgg cgacgatttg cacggcgttg
     4441 ccagagatgg tggcggtggc tttaagccgg tcaggaacca cgatatcgcc atccgcgctg
     4501 gtgatgggga atttaccact ggttccaata ttctgcgctt gcaggttgaa gtgataggcg
     4561 gataccttct ggatagcctg ctgcgcgctt ttaatcaggg tttgggcgct gggagtggta
     4621 tttccgctgt tgttgtcaca cgcggcgagc agaagcacga gagcaagaag cagcgcgagc
     4681 atgatgggga tttttgtttg tgatgttctt tgtcttgtct gcatagctgc cccccttgtt
     4741 tttccaggcc ggttcgcgtt cgtatgttca ctattgtacc ataggctata tgcgcttagc
     4801 ccggaggagg ctcacaccat ttttcctacg tttcttgtat aagggaggct ggtatgctat
     4861 gctcgatggg tggagatatt ccgatgcaaa agggattaga gcatatcatg gagcagaagc
     4921 aggtcaatgt gccgttgcgg gtgcgtttgc gcctgtcggc ggaacgcact cctggggaag
     4981 agggtcgagt accggcgtgg gtggaacaga tggggaaggc agcgttagag gcggcggagg
     5041 tgacgagcgt gggacagcag caggtggatc gcgccaatga gggaatgcgc caggtgcgag
     5101 aggcgaccct gcaatcggca agggcgatca agcggctggg cgaaggcggg caggagatca
     5161 acaataccgt ggtggagctg accgacctga cgacgcgcat gaatctgctg gcgctgaatg
     5221 ccgcgatcga ggcggtgcgc gcgggcgagc agggccaggg cttcgcggtg atcgcgcagg
     5281 agatacgtgc gctgtcggcg cactgtgccg aggctgcgcg caaggtatca gcgcggctac
     5341 acgcgattca gagcgaagtc gcggtggcgt cgaagagtgt tgacgcgagc atcacgcagg
     5401 tggtcgtgca gtgcgaactg gtcacacaga tgggcgtggc gctggatgcc atcggcattg
     5461 tgaccgaaca aatggcgacg ctggcacagg gattgggcgc tacttctccg gcgtatcgtc
     5521 tggaagattc ttcttcactg tattgaccaa ctcgtcaagg tgaaagggct ttgccagaaa
     5581 gtcgtctgca ccgcactcct cgatggcttt tgcggcgtta aagtgcgccg agagtaggac
     5641 cactggaatt tgcctggtct gttcgcggct tttcagctgc ctgcaaatca cccttccatc
     5701 ttcgcctgac aataaaatat caagcagaat caggtcgggt ggatgttcca taggttgtag
     5761 caggctggca ccggtcaggt aggtactcgt ctggtagccc gcgtcatcca gtacaatctg
     5821 tacgacatct aagatatctt gctggtcgtc aatgacccag acatgttctt tcatcgctcg
     5881 tcctcatcta ggtgtgtggc atggtctggt cctgattgac cctgggagaa tgggagggat
     5941 agataagagg ttgaaccttg ccctgtctcg ctttctactc gcatagttcc gcaaaaatga
     6001 tcgtccaggt gtaacgtgtc ggaggatgcg gcatcagggt tgggaaaatc ttcttcgtgg
     6061 ggggcaaccg tggaagagtg acgaggtacc tgccgttgtt tcttcatggt atgagacacg
     6121 ctttgttacc gcccttctac taaataaccc ggccttatgg acgcttcgcc aggatgccga
     6181 aaaagcgcct attaccgcaa acacagtgta ttatataact gatgtcaagc atttttgaag
     6241 attcgagata gaatgaggac tattggtgca ttgatataaa taatggtcaa atgggggccg
     6301 cattgttcac ctaaatatat ttgcgacgcg cgcgctgctt tcgtcgctgc ttctgctgca
     6361 agcgttccag gcgttgtttc tggcgcgggg agggttcgta ttcgcggtag atgcgcgctc
     6421 cgatgcgcca gccgagatag ccgaagacga tggaggcgag caggccgacg atgatcatga
     6481 cggcgcgcac ggtgaacgag agccagccgt gcagagcgtc gaagagggga gtcgcgtaca
     6541 ggcggctgag gatggcgatg ctgagactga gcagcagccc gacgacgacg ccgaggcagc
     6601 agccgagcgg gcctccattg gtttcgcctt gcgcctccgg cggtagctgt gtctcggatt
     6661 gcggcgacgg cgaggatgca attgggtcag aatcagccat tagaatgttc caaactggcg
     6721 gtcgccggcg tcgccgaggc cggggacgat gtagccgatg tcgttgaggc tttcatcgag
     6781 cgccgcgata tagatgtcga tgtcgggatg ggtttgcgag agcttgagca gtccataggg
     6841 agcggcgatg atgcccacgt aacatagacg cgggatgttg cgccgcttca agacgttgat
     6901 ggcatcgata gccgagccgc cggttgccag cataggatcg acggcgtagc agacctggag
     6961 atcgaccttg tgcgggagtt ggttgtagta ttccatggct tccagggtgt gttcgtcgcg
     7021 gcgcaggccc agatgccaga cctgcacgtc ggggatgacc tggcggaatc cttcggccag
     7081 gcccaggccg gcgcgcagga tgggtgtgac gccgatgccg ccggccagcc gctcgccgat
     7141 catgggtgac atgggtgtct cgatctgtct cggctccagc gtgaggttgg aggtcgcttc
     7201 ataggccagc agcgccccga tctctttgac caggttataa aattccggag gcggcgtgtt
     7261 tttgtcgcgc agggcggtca ttttgtgctt cataaccggg tgcgacgaga tatgcacccg
     7321 cggcagttgc atgacttcct cccggctggg ctgcggcacc ccccgcatgg gcggattggg
     7381 acgcccgcca ctggatgtgt tcgtttctgc catacgatag ctcctttgaa gttgttgctc
     7441 tgtagtgtat tttaacaggt ctgggaagag gtttgtagcg ggcaaggttc ttcggtgcgt
     7501 ttgtatgaaa tacagtagtg ttctgggcgg gaatctgcta tgatgagggc atgatagtgg
     7561 gagtttgccg gattacgctg cacttgccgg ggtgtcattc gttaaaagag aaacgccagg
     7621 tcgtcaaatc gctgatggcg cgggtgcgca accgctttga ggtggcgatt gcggaggttg
     7681 aagagcagga tcgctggcag acggctgtgc tgggcgtgac ctgcgtgagt aatagcagtc
     7741 agcatgcaga cgaagtgctt ggccatgtgc agcgttttat tgaggagacg cgccccgata
     7801 tacttgtgac caataacgaa atggagatta tgcactggtg aacatgcgcc ggcgcagttg
     7861 ataagaggga atgtctacag agttagagcc gcaccgttgg aggagtagcc gagtcctcgg
     7921 agtgcgtgtt gacgcggtga cgcgggagca ggcgcttgat gccatagagc agttcgcggc
     7981 gaaacgacgc gccagcctgg aaacgggaga tgtcttagct tgccagcagg ttgtgacggt
     8041 caataccgag tttgtgatgg aggcgcagcg taatagcgcc tttcgcgagg caatcaacgc
     8101 ggcggcgctg gttgttgctg atggcgtcgg cattgtgtgg gcgacgcgct tgcgcggaac
     8161 acctgtgccg gagcgcatca cgggaaccga taccgttatg gatgtagcga aacgctgcgc
     8221 gcggtcgggg taccggctct acctgcttgg ggccgcgccg ggcgtggcgg aactggcggc
     8281 agagcgtctg caggctatcg cgccggggct ggaaatcgcc gggacgtatg ctggatcgcc
     8341 cgcgccggag gaggaagatg gcatcgtgga gcgcctgaat gccgcgcgag ccgatgtgct
     8401 gtgtgtggcc tatggcgcgc ccgcgcagga actctggatt cggcgcaacc tggagcgact
     8461 gcccgttgcg gtggcgatgg gtgtcggcgg cgcgttcgat ttcctgtcgg gccgccagcg
     8521 ccgcgcgccg cgtattatgc agcgcctggg cctggaatgg ctttaccggc tgtaccgcga
     8581 accgtggcgc tggcggcgaa tgctggccat tccccagttt ctgttgtatg tgttgttgaa
     8641 aatccgagga gataaatcgt cgtagggacg cgatgatgta cttcttatga ttttttatga
     8701 ccaagatcgg caagagggtc tggaggagcg atcaagaacg tcatcctgag cgcagcgaag
     8761 gatctgatgc gccggggacc gagatccttc gctgcgctca ggatgacatt cccggctttt
     8821 gttatgaaac gctattatcg gtcacgacgg gcgttaacgg caagcactca ggccgatgaa
     8881 tcggcggtgt gcgcgatcaa tcggcaccta caggtttatc acgttaattg ttgaatgttc
     8941 atcatcgtgt ttctatgacg atgtcatgat tacaaaagtt tgtgtgagta tgtaatgaaa
     9001 gaagaacttc atatagccaa taatccgcta caggcgataa tccgggggtt cgcggggaag
     9061 cgtgtgctgg tgattgggga tatggtcgcc gatgagtacc tggttggcag gccggcgcgc
     9121 atttcgcgtg aagcgcctgt cttgatcctg gaactgtacg aggaacgcac ggttccgggc
     9181 ggggcaacga atgtggccgt gaatgcgcgc tcgctggagg cggaggtctt cctggcgggc
     9241 gtggtgggtg atgatgtgcc ggggcagcgt ttgcggcgcg cggtagatag tctggggatg
     9301 catcaggagg ggttggtgac gcatccgacg cggcctactt cgacgaagac gcgcattatg
     9361 gcgggcagct cgcagattgt gcagcagcat atcgtgcgta tcgaccgcgt tgatacgtcc
     9421 gacctggatg atggcagcca ggcggccatt atcgagtatg taagggcggt tctgccttcg
     9481 atggacgcgg tcgtgctgtc ggactatgag aatggggtta tcagcccgaa aatactcgag
     9541 gtatgtttgc cgatggcgcg cgaattggac aaggtgatcg tggccgattc gcatgagctg
     9601 ctcttacgct tccagggtgt gacggccatt acgcccaacc agccggaggc ggagcggacg
     9661 cttggggtga cgatcaacag ccgcgcggaa ctggacgagg ccgggcagcg gctgctggcg
     9721 ggcagcgcgg cgcagagcat cctgattacg cgcggcgcgg agggcatgag cctgttcgag
     9781 gttggtaagc ctcccctgca tttgcccgtc taccggctcg accacgccag cgagatcgtc
     9841 gatacgaatg gcgcgggcga tacggtcgcg gccaccttca cgctggcgct cacggcaggc
     9901 gcgagcttcg cagaggctgc ctaccttgcc aatgcagcgg cagcgctggt ggtgcggcgt
     9961 ctcggctgcg ccagcaatac accgcaagaa ttgatgagcg cgttgcatga gtgagatgaa
    10021 cgatagaaac gaactagatt cgatgcgaaa agatacgcgc gtgaaaatta aaagccgcga
    10081 cgag
//
LOCUS       DASZAK010001060         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_169155, whole genome shotgun sequence.
ACCESSION   DASZAK010001060 DASZAK010000000
VERSION     DASZAK010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169155"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1440
                     /locus_tag="VGT44_18745"
     CDS             <1..1440
                     /locus_tag="VGT44_18745"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEV2582904.1"
                     /translation="LGGVLVVLLAVFAALTLLPAALAILGTRVNAFPVRLPRFGRARA
                     QAANREIHHGFWYRLSNFVMRYPVRVFVTVLLLMVALGLPFLGVRFSAPDASILPKSV
                     PSRAAYDLLNSRFDVQDTTPILLAVQTTGDALTPDNIRNLYNYVRRIEADPRVARVDS
                     IVSADPRLTLAQYELLYAHPQQITDPYFAAVLKNEVAGNTTMILVISNYPMLDQRSVE
                     LVQTIRNTPPGNAMTTLVDGGTAGNIDYVQSLYSDFPIAALIVAITTYLVLLLFFRSL
                     VLPLKAILMNTLSILASYGMLVVIFQNGFLHSLLGFTPLGFVEASSPILLFCTLFGLS
                     MDYEVFLLSRIQEAFWQTGDNTRSVALGLQRSGGIITSAALIVIVVSGCFATADIILV
                     KALGLGMALAVALDATLVRGLLVPATMRLLGNINWWLPFVGVQRHPFARREGYSEDAE
                     KNPTSTKQWLVQDSIKEPPEGDRKGARLQ"
     gene            1535..2638
                     /locus_tag="VGT44_18750"
     CDS             1535..2638
                     /locus_tag="VGT44_18750"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HEV2582905.1"
                     /translation="MIYDKKYIQLAQLLPIIIVFASCGFPGLTSTAAQLPVVQSTPTQ
                     ASLPPVRFPQDEAPHRDLTEWWYYTGHLNATIHGQLHHYGFELVFFQVLRADFPPIYA
                     SHFAISDVTRGEFHFDQRRQAEVNAVLPNGTTTTGFDVNIGDWSIRGVNGLDHLAAGM
                     SNYTLRIDVRGLKPPVLHNGDGLITFGLGGFSYYYSRTRMAVSGTLVDHGQALAVSGE
                     AWMDHQWGNFVTLGGGGWDWFSIQLANNTELMLYLIRDATGKIVSTYVGYILPDAQER
                     LLPPQSLRVQVLDHWRSSVTGVVYPSGWLVQIHDAQLYATLTLLPELKNQELVVYQST
                     GNSYWEGAVSIVGQDNGSAISGEGYVELTGYQP"
     gene            2798..>3264
                     /locus_tag="VGT44_18755"
     CDS             2798..>3264
                     /locus_tag="VGT44_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582906.1"
                     /translation="MGVCCEKQRQVHEEAEIQTHLQEDTFSIIERVARIVANVRGTKT
                     DYAHLASELAPVFPFDLFGVVLLRYDRQAVRVTACTREAGRWVAHYHQHPLADSMLER
                     FVGQDEAALAKPVLPAQVDGEEERGMLIRIYPGGVDGSPAECGDALSGYPRLRA"
BASE COUNT          611 a          984 c          937 g          732 t
ORIGIN      
        1 ctgggcggtg tgctggtcgt cctgctggcc gtcttcgccg ccctcacgct gctgcccgcc
       61 gcgcttgcca tcctgggaac gcgcgtcaat gcctttccgg tgcgtctccc acgattcgga
      121 cgcgcaaggg cgcaggcagc caatcgcgaa atccatcacg gcttctggta tcgcctctca
      181 aattttgtga tgcgctaccc cgtgcgcgtc tttgtgaccg tcttgctgct gatggtcgcg
      241 ctcggtctgc ccttcctggg tgtgcgcttt tccgcgccgg atgcctcgat cctgccgaaa
      301 agcgtgccgt cgcgcgccgc ttatgatctg ctcaacagcc gcttcgacgt gcaggacacg
      361 actcccatcc tgctggcggt gcagacgacg ggcgatgcac tgacacccga caatatccgc
      421 aacctctata actatgtgcg gcgcatcgaa gccgatccgc gcgtggcgcg cgtcgatagc
      481 atcgtcagcg ccgacccgcg cctgacactc gctcaatacg agctgctcta cgcgcacccg
      541 cagcagatca ccgatcccta cttcgcggct gtcttgaaga acgaggtggc gggcaatacc
      601 accatgatcc tggtcatcag caactatccc atgctcgacc agcgttccgt cgaactggtg
      661 cagaccatcc gcaacacgcc gcccggcaat gccatgacga cgctggtgga tggcggcacg
      721 gcgggcaaca tcgattatgt gcaatcgctc tacagtgatt ttcctatagc cgcgttgatc
      781 gtagcgatca cgacctatct cgtgctgctg ctcttcttcc gctcgctagt cctgcccttg
      841 aaagccattc tgatgaacac gctctcgatc ctggcgagct atggcatgct ggtggttatt
      901 ttccagaatg gtttcctgca ttcgctgctc ggattcacgc cgctcggctt tgtcgaggcg
      961 tccagtccca ttctgctatt ttgcacgctc tttggtctgt cgatggatta cgaggttttc
     1021 ctgctgtcgc gcatccagga ggcattctgg cagacgggcg ataatacgcg ctccgtggcg
     1081 ctgggcttgc agcgcagcgg cggcatcatc accagcgcgg cgctgatcgt gatcgtcgtg
     1141 agcggttgtt tcgccaccgc cgatatcatc ctggtcaagg cgctgggcct gggcatggcg
     1201 ctggccgtcg cgctcgatgc cacgctggtg cgcggcctgc tcgttcccgc cacgatgcgg
     1261 ctgctcggca acatcaactg gtggctgccc ttcgtcgggg tgcagcgcca tcccttcgcg
     1321 cgacgcgagg gctatagcga ggacgctgaa aagaacccca cctctacaaa gcaatggctg
     1381 gtccaagatt ccatcaaaga acccccggag ggcgaccgca agggtgcgcg cctacaatga
     1441 tccgacggga gaaacgcgcc gaatcacttc gggatcatgg taggggcgca cccttgcggt
     1501 tgccctccgg ggattacccg agttggagga aacaatgata tacgacaaaa aatacataca
     1561 acttgctcaa ttattgccaa taatcatcgt gttcgcctcc tgcggttttc cggggctgac
     1621 ctccacagcc gcgcaactgc cggtcgtgca gtccacgccg acgcaggcgt ccctgcctcc
     1681 tgtgcgcttc ccccaggatg aagcgccgca ccgcgacctg acggagtggt ggtattatac
     1741 cggccatttg aacgcgacaa tccatggaca actgcatcac tatggcttcg agctggtctt
     1801 cttccaggtc ttgcgcgccg acttcccgcc catctatgcc tcgcattttg ccatcagcga
     1861 tgtgacgcgc ggcgaatttc actttgacca gcgccgccag gccgaggtga acgcggtcct
     1921 gcccaatggc acgaccacca ccggctttga tgtgaatatt ggcgactggt cgattcgcgg
     1981 cgtgaatggt ctggatcatc tcgcggccgg catgtcgaac tataccctgc gtattgatgt
     2041 tcggggtctc aaaccacccg tgctgcacaa cggcgatgga ctcattacct tcggcctggg
     2101 cggcttttcg tattactact cgcggacgcg catggccgtc tccggtacgc tggtcgacca
     2161 tgggcaagcg ctcgcggtga gcggggaagc ctggatggat caccagtggg gcaattttgt
     2221 cacattgggc ggcggtggct gggactggtt cagcatccag ctcgccaaca acaccgagtt
     2281 gatgctctat ctcattcgcg acgctaccgg gaagatcgtc tccacctacg tcggctatat
     2341 cttgccggac gcgcaggaac gcctcctccc tcctcaatca ctgcgtgtgc aggttctcga
     2401 tcactggcgc agttccgtta ccggcgttgt ttatccctct ggctggcttg tgcaaattca
     2461 tgacgcgcag ctctatgcta ccttgacgtt gctacccgag ctcaaaaacc aggaactggt
     2521 cgtttatcaa tcgaccggga acagctattg ggagggcgcg gtatccatcg tcggccagga
     2581 caatggcagc gcgatcagcg gcgagggcta cgtggaattg acggggtatc agccctaatt
     2641 tgtgttatac tgttcaggta ccttcgggct atacaattcc gtaatttgaa ggatttgtat
     2701 acatacgcta acattgtcat gcatcgtatg ctacaatgtt taaccgtatc tggttgtgtt
     2761 gatccgtata ccgtcaggtt gctttaggat aagcagggtg ggagtgtgct gcgagaagca
     2821 gaggcaggtc cacgaggagg ccgagattca aacgcatttg caggaagata cattttctat
     2881 aatagagcgt gtcgcgcgca tcgttgcgaa tgtgcgcgga acgaaaacgg actatgcgca
     2941 cctggcgtca gagcttgctc cagtatttcc atttgatctc tttggagtcg tgctgttgcg
     3001 ctatgatcgc caggcggtgc gcgtaaccgc ctgtacacgc gaagcaggca gatgggtagc
     3061 gcattaccac cagcatccac tggccgattc gatgctagaa cgctttgtcg ggcaagacga
     3121 ggcggcactg gcgaagcccg tattacccgc acaggttgat ggcgaagagg aacgagggat
     3181 gctgattcgc atctaccctg gcggagtgga cggttcacct gccgaatgcg gcgacgcatt
     3241 aagcggctat ccgcgcctgc gcgc
//
LOCUS       DASZAK010001061         2484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_702592, whole genome shotgun sequence.
ACCESSION   DASZAK010001061 DASZAK010000000
VERSION     DASZAK010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702592"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VGT44_18760"
     CDS             complement(<1..1112)
                     /locus_tag="VGT44_18760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEV2582907.1"
                     /translation="MLTLRRIFVVIVLALLVAACAPSGSSGTSSTPTPASNSTVVTTT
                     PNHVASTPTHVGTTPQASATAKPGASPTPVVDTSPLTCAANILGSHPDANCQTPRSLR
                     VAYGVESLMQRGYTGSGQTVVDIVSFGSPILQRDMNIFDQQFGLPPIAIQVIAPIGTA
                     PFNPANKLMQGWAVETELDVQIIHAIAPGAHIVVLTSPVAEVEGTTGLPQFLQLEQYA
                     INHHLGSIISQSFGASEATLKDSASQQQIQQWDAFYKQATTQQGMTFLGASGDNGATD
                     FTDANASVLATTPTTSFPTDDPWVTSTGGTTLDRTQNGFAEMAWNNSLGASGGGFSAF
                     FSTPSYQQSLSTVVQQLLNKRRGVPDVAADADPNTGL"
     gene            1264..>2484
                     /locus_tag="VGT44_18765"
     CDS             1264..>2484
                     /locus_tag="VGT44_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582908.1"
                     /translation="MIQQPVPPPEYDPTIQVSDALLAEKLGDSALVGKWFAFLRADWL
                     NRSGMDRLLSLAGVTGVEHVTPRSAILFRGREELTLPGATIAPTPLPASRLISLSSDK
                     ALYRAHHDTVRLLIAAPQQPGETLKLRLRLGSNIYGEYPLTLDAFGLHLWSLSGLPEG
                     AYEAELEGIEDIVCRFEVAEYRLAPLNAELLDQQLSGEALRYTLAVSAFGQPYTGTIE
                     VELQERDRRVDQREKLSCNREGQCRGVVKLTGAGPYTLNIYAGERTATVALKGSEQQR
                     RETLVISELGEIRSISLLPLPQSNACRGVYVSRGGSNDAPFVMQRAIGSEAEITPRVD
                     IEQLKVIIVDPARGSLDEREWTTLKAGQPILLPVPAPYGVVLLGAFIDARAWEGWCAV
                     LRSPALQTQCEAPAQ"
BASE COUNT          504 a          740 c          743 g          497 t
ORIGIN      
        1 aggccggtat tcggatcagc atccgccgcg acatctggca cgccgcgccg cttattcaac
       61 agttgctgca cgactgttga caggctctgc tggtacgacg gcgtggaaaa gaacgcgctg
      121 aatccgccac cactcgcgcc caaagaattg ttccatgcca tctctgcaaa gccgttctga
      181 gtgcggtcca gcgtcgtgcc tcccgtactc gtcacccagg gatcatccgt ggggaagctg
      241 gtggttggcg tcgtggcaag cactgatgca ttcgcgtcgg taaagtcggt cgcgccatta
      301 tcgcccgagg cgcctaagaa ggtcatgccc tgctgcgtcg tcgcctgctt atagaaggca
      361 tcccactgct gaatctgctg ctggctcgcg ctgtccttca gcgttgcctc ggacgcgcca
      421 aagctttgtg agatgatcga acccaggtga tggttgatgg cgtattgttc cagctggagg
      481 aattgcggca ggccggtcgt cccctcgacc tcggcgacgg ggctggtcaa caccacgata
      541 tgcgcgcctg gagcgatggc gtggatgatc tgcacatcca gttcggtttc gaccgcccaa
      601 ccctgcatca atttattggc aggattgaaa ggcgccgtac caatcggtgc aatcacctgg
      661 atcgcgatag gcggcaggcc aaactgctgg tcgaagatat tcatatctcg ttgcaatatc
      721 gggctgccga acgagacgat atcgaccacc gtctgcccgc tgccggtgta gccgcgctgc
      781 atcagcgact caaccccgta agcaacgcgc agggaacggg gcgtctggca gttcgcgtcc
      841 gggtggcttc ccagaatgtt cgccgcgcag gtcaatggcg atgtatcaac aaccggcgtc
      901 ggcgaagcgc caggtttagc agttgcggat gcctgtggcg ttgttccaac atgggtcggt
      961 gtggaagcta catgatttgg tgtggtcgtc acaacagtag agttagaagc gggtgtaggt
     1021 gtgctgctcg ttccgctact gccagatggc gcacaggctg cgaccagcag cgccagtaca
     1081 attacaacga agatacgtcg aagcgtaagc atgaacaggt actttctaac tttacaagag
     1141 tctttcaata cgtacattct agcaagtggc cagggcgatg gcaagcaact tccccttgta
     1201 cttttcgtag agatggtgta ataataatcc ttgcaataga gaacatgatc ggagagcagt
     1261 gctatgatcc aacaaccagt accaccgcct gaatatgacc ccacgataca agtttccgac
     1321 gctttgttgg cagaaaagct gggcgacagt gcgctggtcg gcaaatggtt tgcgtttttg
     1381 cgcgccgatt ggctcaaccg ttcaggaatg gatcgcctgc tctccctggc cggtgtaacg
     1441 ggtgttgagc atgtcacacc ccgctcggct atccttttcc ggggccggga agaactcacg
     1501 ctgccaggcg ctaccatcgc ccccacgccc ctgcccgctt cgcggttaat atcgttaagc
     1561 agcgacaaag cgttataccg cgcccaccac gacacggtgc gcctgctgat cgccgcgccg
     1621 caacagccgg gcgaaacgct gaaattgcgt ctgcgcctgg gcagcaacat ctatggcgaa
     1681 tatcccctca cgctggatgc ctttggcctc catctctggt cgctatcagg cctgcctgaa
     1741 ggcgcatacg aagcagagtt ggaaggcatc gaagacatcg tttgccgttt cgaggtcgcg
     1801 gaatatcgcc tcgcgccgct caacgccgag ctgctagacc agcagttgag cggggaagcg
     1861 ctgcgctata ccctggccgt cagcgccttt gggcaaccct acactggcac aatcgaggtc
     1921 gagctacagg aacgcgaccg gcgcgtcgac cagcgcgaaa agctgtcctg caatcgcgag
     1981 ggccaatgtc gcggcgttgt gaagctcaca ggagccggac cgtataccct caacatctac
     2041 gcgggagagc gcacggcaac cgtcgccttg aagggatcgg aacagcagcg gcgtgaaacg
     2101 ctggtcatca gcgaactggg cgagatccgt tcgatcagcc tgctgccgct gccacagtcg
     2161 aatgcttgcc ggggcgtcta cgtatcgcgc ggcggcagca acgacgcgcc gttcgtcatg
     2221 cagcgcgcca tcggcagcga ggcggagatc acgccccgcg tcgatatcga acagctcaag
     2281 gtcatcatcg ttgatccggc gcgcgggtcg ctcgacgagc gcgaatggac aacgctgaaa
     2341 gccgggcagc ccattcttct gccggttccc gcgccatatg gggtcgtgtt gcttggcgcg
     2401 ttcatcgatg cccgcgcctg ggagggctgg tgcgccgtcc tgcgctcgcc agcgctgcaa
     2461 acacagtgtg aagcgcctgc acag
//
LOCUS       DASZAK010001062         2580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_632965, whole genome shotgun sequence.
ACCESSION   DASZAK010001062 DASZAK010000000
VERSION     DASZAK010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2580
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_632965"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1559
                     /locus_tag="VGT44_18770"
     CDS             144..1559
                     /locus_tag="VGT44_18770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2582909.1"
                     /translation="MTPAQTMLEKIARQACQFICCDAALLVIDCPAPELRHPLLALFP
                     ATTPPAARMFGASRLASLLRSGEARAAYDIACQRGEIQYCELTVEGERECTQSLAVAP
                     LAGPAGILGYVLLANASGFTRGDDALLLSFVKENLYNVESAVRTLLLASLLRAERLLV
                     KNAGTDGGQHQLPEMLNSISMVSHELRSPLAAIKGYAGLLQAYSGAGPQQDSLPEEDA
                     PFSLTPAQQQRYLDMILEESQRLEELIADLLDASRMQSGKLTLSFGAVDVGEACERAA
                     RRARRRIEQVRHARPRLECQVTPHLPPMYADAKRLQHILDNLLDNAIKYSPQGGTIEL
                     LAGIFPQQYESAQRSSHDRNKMNAQTITIVVRDQGIGIPADQRARLFQPFSRIDNPQV
                     PRIQGVGLGLYITRQLIEAMHGTIDISGQVDRGTVVTLCFPAAEQSYLNDIASTYVLP
                     SVMSHSLTLSHEINSRMRFYC"
     gene            1670..1867
                     /locus_tag="VGT44_18775"
     CDS             1670..1867
                     /locus_tag="VGT44_18775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEV2582910.1"
                     /translation="MQLNKGHGRGFASMTPEKKREIASKGGKAAHAMGTAHKWTSEEA
                     QAAGRKGGSISRRRSRSVARS"
     gene            complement(1951..>2580)
                     /locus_tag="VGT44_18780"
     CDS             complement(1951..>2580)
                     /locus_tag="VGT44_18780"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEV2582911.1"
                     /translation="SIFVVPLDSSQHASTISQLIGNLFSSSNSQPGIIDTMQQSITPT
                     NGPTSGDSGGSCLGTDIWSTCALYVTDSGVEGTGQMQRPIVGSVISQYFGHLEYQYWC
                     GCWKPHTGIDLAAPYGTPIMAADSGQVIWVGWDWSGLGWAVKISHGHYIATIYGHMDH
                     YIVKVGESVSKGQTIGYEGSTGASTGPHLHFMVLVHNIWVNPLLYVALP"
BASE COUNT          567 a          710 c          739 g          564 t
ORIGIN      
        1 caaagaacgg ccagtggcgg gtggagaccc ttgcggtcgt cctgagttta tatgcggaag
       61 tccgcgttaa ttattgaatt ttcatcattc cccatgctat gtagagtgtc gttattcggg
      121 ggagataggt aggggatcga ctgatgacac cggcgcaaac gatgctcgaa aaaatagcgc
      181 ggcaggcgtg ccaatttatt tgctgcgatg ccgcgctgct ggtgatcgac tgccccgcgc
      241 ctgaattacg ccatcccttg cttgccttat ttcctgcgac gactccccct gctgctcgta
      301 tgtttggcgc aagtcgcctg gcgtcgttgt tgcgcagtgg ggaggcgcgc gcggcctacg
      361 atatagcctg ccagcgcggg gagattcagt attgcgagct gacggttgag ggcgagcggg
      421 aatgcacgca atctctcgcg gttgcgcccc tggctggccc ggctggcatc ctcggatacg
      481 tcctgctggc gaatgcctcc ggttttacgc ggggagatga cgcgctttta ctttcgtttg
      541 taaaagagaa cctgtacaat gtcgagagcg ctgtgcgcac actgctgctt gccagcctct
      601 tgcgggctga gaggctcctg gtgaaaaacg ctggcacaga cggcggtcag catcaattgc
      661 cggagatgct caacagcatt tcaatggtca gccatgaatt acgttcgccc ctggcagcta
      721 tcaaaggcta tgccggtttg ctccaggcat atagcggcgc ggggccgcag caggatagct
      781 tgccggagga agatgccccg ttttccttga cgcccgcgca acagcagcgc tacctggata
      841 tgattctgga agaatcgcag cggcttgaag aattgatcgc cgatttgctg gatgcgtcgc
      901 gcatgcagtc tggcaagctc accttgagtt ttggcgcggt agatgtgggc gaagcctgcg
      961 agcgcgcggc tcggcgcgcc cggcggcgca tcgaacaagt gcgccacgca cgaccccggc
     1021 tggaatgcca ggtcacacca catctgcccc ctatgtacgc ggatgcaaaa cgcctccaac
     1081 atattctaga taatctgctc gataacgcca tcaaatactc accgcaaggt ggtaccatcg
     1141 agctactcgc cggaatcttt cctcaacaat acgaatctgc tcagcgctct tcacatgacc
     1201 ggaacaagat gaacgcgcag acgattacga ttgttgtgcg cgatcaaggc atcggtatcc
     1261 cggcggatca gcgcgcgcgc ctcttccagc cgttcagtcg catcgataac ccgcaggtgc
     1321 ctcgcatcca gggtgtcgga ttgggcctct atatcacccg ccagctcatc gaggcaatgc
     1381 atggaaccat cgacattagc ggccaggtgg atagaggaac ggtggtcacg ctttgttttc
     1441 ctgctgctga gcaatcgtat ctcaacgata ttgcaagtac atacgtatta ccatcagtga
     1501 tgtctcacag cctgacgtta tctcacgaga tcaacagtag gatgaggttt tattgttgac
     1561 ttgtgctttt atggttcagg ctggtatgct tgttatgtag atacatgaca gtgagttggc
     1621 atcatgtatg tacaaaccga ccattacaag aaagcaagga gaacacacaa tgcagttgaa
     1681 caaaggacat ggtcgaggtt ttgcgtccat gactccagag aagaagcgag aaatcgccag
     1741 caagggcggc aaagcagccc acgccatggg aactgcgcat aagtggacca gcgaagaggc
     1801 ccaggcagcc ggccgtaaag gcggttcgat cagtcgtcgc cggtcaagaa gcgtcgcccg
     1861 gtcgtaatcg taggaatcaa cgatggtgat ttgagaaatg ggcaatgtgg cgctgccaca
     1921 ttgcccattt ctcaaatcac catcgctccg ttatggtaat gcaacgtaca ggaggggatt
     1981 gacccagatg ttatgcacca gcaccatgaa gtgcagatgc ggacccgtcg atgcgccggt
     2041 gctgccctca tacccgatcg tctgcccttt ggagacgctc tcgccgacct tgacgatgta
     2101 gtggtccata tggccgtaga ttgtcgcaat gtagtgtcca tggctgatct ttaccgccca
     2161 ccccaacccc gaccagtccc agcccaccca gatcacctgt cccgaatcgg ctgccatgat
     2221 cggcgtgcca tagggggcgg ccaggtcgat gccggtatgc ggcttccagc agccgcacca
     2281 gtactggtac tccaggtgac caaagtactg tgagatcacc gatcccacaa tcgggcgctg
     2341 catctgtccg gtaccttcca caccgctatc cgtcacatac aaagcgcagg tactccagat
     2401 atcggtaccc aggcatgacc ctccgctatc gccgctggtg ggaccattgg taggggtgat
     2461 cgactgttgc atagtgtcga tgatgccggg ctggctgttg ctgctagaaa acaggttgcc
     2521 gataagctgg gagatagttg aagcatgctg gctgctgtcg agtggcacga cgaatatgga
//
LOCUS       DASZAK010001063         4734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_346538, whole genome shotgun sequence.
ACCESSION   DASZAK010001063 DASZAK010000000
VERSION     DASZAK010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346538"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..839)
                     /locus_tag="VGT44_18785"
     CDS             complement(108..839)
                     /locus_tag="VGT44_18785"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEV2582912.1"
                     /translation="MIVFQYHPDIMCRFPGIVGGVIYARGMTNGPTPAALREAFEAEQ
                     QAVLRRIGETSLSQLPSLAAWRGAFRDFGVEPTQYRSAAEALLRRLTKKGDIPSINTL
                     VDIGNLVSIRYALPTAVFDTRAIQGTLTVHVADGSERYTTLGQEEVDHPAPGEVVFSD
                     ETGLVMARRWCWRQSEQSAAQSDTTTAIITIEGHHAAAHRVIKAALLDLLALLNAYAG
                     GSYMYDILCAGEAGHAVFVIECSNP"
     gene            complement(844..2091)
                     /gene="trpB"
                     /locus_tag="VGT44_18790"
     CDS             complement(844..2091)
                     /gene="trpB"
                     /locus_tag="VGT44_18790"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV2582913.1"
                     /translation="MQLQETDKAYRHQYYPDERGRFGAFGGKFVPESLMQPLAELEAA
                     YEQARSNTAFQQELRSLLRSFVGRPTTLHYAPRYSAMVAPGIKVYLKREDLAHTGAHK
                     NNNALGQGLLAKRMGKQRIIAETGAGQHGVATATVCAMLGLECIVYMGEEDIHRQSLN
                     VFRMKLLGAEVRSVTSGARTLKDAINEAIRDWVTNVESTFYLIGSVVGPHPYPRIVRD
                     FQSIIGIEAREQILAQEGRLPDLIVACVGGGSNAIGIFYPFAADAGVRLLGVEAGGAG
                     DAGLRAGQHAATLVAGRPGVLHGAYSYLLQNADGQVIDTHSISAGLDYPGVGPEHSYL
                     KETGRATYVSASDETALRGLQALSRSEGIIPALEPAHALGYLLEMGERGEIAPGTLAI
                     LNLSGRGDKDMLTVAKALNVTLE"
     gene            complement(2159..3565)
                     /gene="trpCF"
                     /locus_tag="VGT44_18795"
     CDS             complement(2159..3565)
                     /gene="trpCF"
                     /locus_tag="VGT44_18795"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006945.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HEV2582914.1"
                     /translation="MFLDRIVTQTRLDLEQRKREQLQEELERRAAAQPAPLDLYEALR
                     AGSWPAVRLIAEVKRASPSKGLLAPHLDPLEAARVYEANGAAAISVLTEPHFFLGSPE
                     HLSAIKRTVHVPVLRKDFIFDDYQVYEARAWGADAILLICAILDDRQLLRLHSLAREL
                     GMRCLVETHTAEEARRAVEAGASIIGINSRDLATFEMHPRLARDLRPLIPADRVVVAE
                     SGIHNAADARRLARCDVQAMLVGESLVVSDDIPAQMQALLHGANESAQIKICGLLTEE
                     HARTAIEAGADMLGLIFYEPSHRYISPHDARALRERIEPLRNTIDLVGVFVNREADYI
                     NDIADLIGLDIVQLHGDETPEFCHRIQRPVIKALHLNNREDAGRVKAYHQASWRILLD
                     TPTPQWGGTGATHDWSLARSIAQITPLLLAGGLNPENVREAMRRVRPWGVDVSSGVET
                     NKVKDVEKIRAFIRNARQ"
     gene            complement(3569..4612)
                     /gene="trpD"
                     /locus_tag="VGT44_18800"
     CDS             complement(3569..4612)
                     /gene="trpD"
                     /locus_tag="VGT44_18800"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV2582915.1"
                     /translation="MIREAIGHVAAGAVLAEDEAAAVMEEIMTGVATPSQMGAFLTAL
                     RLRPGGETVEEIAGMARVMREKAVQVHLDERTAARALDTCGTGGDGAGTFNVSTGAGI
                     LAAAAGAQIAKHGNRSATSRCGSADVLEAMGMRIDLSPEQVASCVQQVGFGFMFAPAY
                     HPAMKHVGPTRREIGIRTVFNILGPLTNPAHTRYQALGVADGSLLRKMGEALLHLGCQ
                     RALIIHGEDGIDECSLSAPTRICEVRKGEGLREYTITPEEVGLASISDRQAFQGGDPA
                     FNASLLRDLLRGQTHSPAADMLCLNTGAALLANEQVGSLKEGVRLAQSVLREGKAARK
                     LEEVAACSTALAR"
     gene            complement(4609..>4734)
                     /locus_tag="VGT44_18805"
     CDS             complement(4609..>4734)
                     /locus_tag="VGT44_18805"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582916.1"
                     /translation="LKHRAFPVEGVQFHPESIMTPTGKDLLQNFLTIYAAERSSI"
BASE COUNT          782 a         1535 c         1497 g          920 t
ORIGIN      
        1 ccgtaggtgc cggttcaccg cgcacaccgc cgattgatcg gccaccatgg gcgttaacgg
       61 caaccaaccg ggccgatcaa tcggcggtgt gcgcggtgaa ccggcaccta cgggtttgag
      121 cattcaatga cgaaaaccgc gtgaccagcc tcacccgcgc acaagatgtc atacatataa
      181 ctccctcctg cgtaggcgtt cagcagcgcc agcaggtcta gcagcgcggc cttgataaca
      241 cggtgcgcgg cggcatgatg gccttcgatg gtgatgatgg ccgttgtggt atccgactgg
      301 gcggcgctct gctcgctctg tcgccagcac cagcggcgcg ccatgaccag gccagtctca
      361 tcgctgaaga ccacctcacc tggcgcgggg tggtcaacct cttcctgacc aagcgtcgtg
      421 tagcgttcgg acccatcggc gacatgcacg gtcagcgttc cctggatagc gcgcgtgtcg
      481 aagacggcgg tgggcagggc atagcgaatg ctgaccaggt tgccgatatc gaccagggta
      541 ttgatgctcg gaatgtcgcc cttcttggtc aggcggcgca gcaacgcctc tgccgcgctg
      601 cgatactgcg tcggctcgac gccaaagtcg cgaaatgcgc cgcgccacgc cgccagcgaa
      661 ggcaactggc tcaacgaagt ctcaccaatg cgccgcagta ccgcctgctg ctccgcctcg
      721 aatgcctcgc gcagcgccgc aggggtcggc ccgttggtca tgccccgcgc gtagatcacg
      781 ccgcccacaa tgcctgggaa gcggcacatg atatcgggat gatattgaaa gacgatcatg
      841 atgttactcc agggtgacgt tcaacgcttt ggcgacggtc agcatgtctt tatcgccgcg
      901 ccccgacagg ttcaggatcg ccagcgttcc gggtgcgatc tcgccgcgtt cgcccatttc
      961 caacaggtat cccagggcgt gggcgggttc gagcgcgggg atgatgccct cgctgcggct
     1021 gagcgcctgc aggccgcgca gcgcggtttc gtcgctggcg gagacatagg tagcccggcc
     1081 tgtctctttg agatagctgt gttccggccc gacgccgggg taatccagcc ccgccgagat
     1141 gctgtgcgtg tctatcacct gaccgtcggc gttttgcagc aggtagctat atgctccatg
     1201 cagtacgcct ggcctgccag cgacgagcgt agctgcgtgc tgccctgccc gcaatccggc
     1261 atctcccgcg ccgcccgcct cgacgccgag caggcgcacc ccggcatcag cggcgaaggg
     1321 atagaagatg ccgatggcgt tgctgccgcc gcccacgcag gccacgatga gatctggtaa
     1381 gcggccttct tgcgccagga tttgctcgcg cgcctcaatg ccgatgatgg attggaaatc
     1441 gcgcacgata cgcgggtagg gatgcggccc gacgacggag ccaatcagat aaaaagtgct
     1501 ttcgacgttc gtcacccagt cgcggatggc ctcgttgatg gcgtctttca acgtgcgggc
     1561 gccgctcgtg acagagcgca cctcggcgcc cagcagcttc atgcggaaga cgttgagcga
     1621 ttgccggtga atatcctctt cgcccatgta gacaatgcat tccagcccca gcatggcgca
     1681 gaccgtcgcc gtcgccacgc cgtgctgccc cgcgcccgtc tccgcgatga tgcgctgctt
     1741 gcccatgcgt ttcgccagca agccctgtcc cagcgcgttg ttgttcttgt gcgcgccggt
     1801 gtgcgccagg tcttcccgct tcaaatatac cttgatgccg ggcgcgacca tcgccgagta
     1861 gcgcggcgcg tagtgcagcg tggtcggtcg ccctacaaag ctgcgtaaca gcgagcgtaa
     1921 ctcctgttgg aaggccgtat tgctgcgcgc ctgctcgtag gccgcttcca gttcggcgag
     1981 cggctgcatc aggctttcgg ggacaaattt accgccgaac gcgccaaatc gcccgcgttc
     2041 atcagggtaa tactggtgcc ggtaggcttt gtcagtttct tgtaattgca ttgtcgtatg
     2101 ttgcctttct caatcgactt gccgacccac cccggatgcc cacaaggggt ggggatcgtt
     2161 actgccttgc attcctgata aacgcccgaa tcttctcaac atccttcacc ttatttgtct
     2221 ccacgccgct gctgacatcg actccccagg gacgaacccg gcgcatagct tctctcacat
     2281 tctcaggatt tagcccacct gccagcagga gcggtgtgat ctgcgcaatc gagcgagcca
     2341 gcgaccagtc gtgggttgcg ccggtgccgc cccattgcgg cgtgggcgtg tcgagcagga
     2401 tgcgccagct tgcctgatgg tacgctttga cccgcccggc atcctctcga ttattcaggt
     2461 gtaatgcctt gatgacgggt cgctgaatac gatggcagaa ctctggcgtt tcgtcgccgt
     2521 gcaactgcac gatatccagg ccaatcaggt cggcgatgtc gttgatataa tcggcctcgc
     2581 ggttgacgaa gactccgacg agatcgatag tgttgcgtaa tggctcgatg cgctctcgca
     2641 gcgcgcgcgc gtcatgcggc gagatgtagc ggtggctcgg ctcatagaag atcaggccga
     2701 gcatatccgc gcctgcttcg atggctgtcc gcgcgtgttc ttccgtgagc aacccgcata
     2761 ttttgatctg cgcgctttca ttcgcgccat gcaacagcgc ctgcatttgt gccgggatat
     2821 cgtcggaaac gaccagcgat tcgccgacca gcatggcctg gacatcgcag cgcgccaggc
     2881 ggcgcgcgtc tgccgcgtta tggatgccac tttcggcgac gaccacgcga tccgccggga
     2941 tgagcgggcg caggtcgcgc gccagccggg gatgcatttc gaacgtcgcc aggtcgcggc
     3001 tgttgatgcc gatgatgctc gcgcccgcct cgaccgcgcg ccgcgcctcc tcagccgtgt
     3061 gcgtctccac caggcagcgc atccccagtt cgcgcgccag cgagtgcagt cgaagcaact
     3121 ggcgatcgtc aaggatggcg cagatcagca ggatggcgtc cgcgccccac gcccgcgctt
     3181 catagacctg gtagtcgtcg aagatgaagt ctttgcgcag cacaggcaca tgcacggtac
     3241 gtttgatggc gctcagatgc tccggcgaac cgagaaagaa atgcggctcg gtcagcacgg
     3301 agatagcggc ggctccattg gcctcgtaga cgcgcgctgc ctccagcggg tcgaggtgtg
     3361 gggcgagcag ccccttggag ggcgaggcgc gtttgacctc ggcgatcaac cggacggctg
     3421 gccaggaacc cgcgcgcagc gcctcgtaca ggtcgagcgg cgctggctgc gcggcggctc
     3481 gccgttcgag ttcttcctgt agctgctcgc gtttgcgctg ctccaggtcg aggcgcgtct
     3541 gtgtgacgat gcggtcgaga aacatgcgtc accgtgccag cgctgttgag caggccgcca
     3601 cttcttccag tttgcgcgcg gccttacctt cgcgcaggac gctctgggcc agccggacgc
     3661 cttccttcag cgagccaact tgctcgttag ccagtagcgc cgcgcccgta ttcaggcaga
     3721 gcatatcggc ggccgggctg tgcgtttgcc cgcgcagcag gtcgcgcagc aggctggcat
     3781 tgaaggccgg gtcgccgccc tggaatgcct ggcggtcgct gatgctcgcc aatcctacct
     3841 cttcgggagt gatggtatat tcgcgcagcc cctcgccttt gcgcacctcg cagatgcgcg
     3901 tgggcgcgct gagcgagcat tcgtcgatcc cgtcttcgcc gtgaataatc agggcgcgct
     3961 ggcagccgag gtgcagcagc gcctcgccca tcttgcgcag cagcgagccg tcggccacgc
     4021 ccagcgcctg gtagcgcgtg tgggcaggat tggtgagcgg gccgaggatg ttgaagacgg
     4081 tgcgaatgcc aatttcgcgg cgcgtcgggc cgacatgctt catggcgggg tgataggccg
     4141 gggcgaacat gaagccgaaa ccgacctgct gcacacagct ggcgacctgt tccgggctga
     4201 ggtcgatgcg catgcccatc gcttccagca catccgcgct gccgcagcgg ctggtggccg
     4261 agcggttgcc atgtttggcg atctgcgcgc ccgccgccgc cgccaggatg cccgcgccgg
     4321 tggagacgtt gaaggttccc gcgccgtcgc cgcccgttcc gcaggtgtcc aacgcccgcg
     4381 ccgccgtgcg ctcgtccagg tggacctgca ccgccttctc tcgcatcacg cgcgccattc
     4441 ccgcgatttc ttcgacggtt tcgccgccgg gtcgcaggcg cagcgcggtc aggaacgcgc
     4501 ccatctgcga tggtgtggca acccccgtca taatctcttc catgacggcg gcggcctcgt
     4561 cttccgccag tacagccccg gcggcgacat ggccaattgc ttctcgaatc atatactgct
     4621 cctctctgcg gcgtaaatag tcaggaaatt ctgtaacagg tctttgccgg tgggcgtcat
     4681 gatcgactcc gggtggaact gtacgccctc caccgggaag gcgcgatgct tgag
//
LOCUS       DASZAK010001064         7087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_239660, whole genome shotgun sequence.
ACCESSION   DASZAK010001064 DASZAK010000000
VERSION     DASZAK010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7087
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239660"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..982
                     /locus_tag="VGT44_18810"
     CDS             119..982
                     /locus_tag="VGT44_18810"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEV2582917.1"
                     /translation="MSWQPQQGQPPQSAPQQQPAADLPEHARQRLMQMRGDATRRGLF
                     TSDLSVNEFLLTREAGFAPLGLVMGSSIYHIGINIANWNKNQEMQVMSQAMYHARNLA
                     MTRMQEEANVLSADGVIGVRLIVNRYQWGEAIAEFMAIGTAVRALSGQSFRNARGLPF
                     TSDLSGQDFWTLLRAGYRPVSMVMGSCVYHVAHMGLRQAMKTVGQNIEMVNYTQALYD
                     ARELAMERMQAEAMSFGAQGVVGMRVQEGSHGWESHVIEYFAVGTAVIPISEEHQVPP
                     PTMTISLNDPS"
     gene            994..2226
                     /locus_tag="VGT44_18815"
     CDS             994..2226
                     /locus_tag="VGT44_18815"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2582918.1"
                     /translation="MDSKQPPNARSLWRNGDFLLLWSGQSVSVLGTNISALALPLLVL
                     AVTHSPAQAGLIAAVQQLPYLLFSLPAGALLDRWDRKKTMIRCDLLRWIALGSVPLAY
                     AFGHLTLAQFYIVAFVDGSAFVFFSIAQIAALPQVVAKDHLPRAYSLTTMSEYAGQLL
                     GPSLAGFIIGLAPVVVAGAIMVYLLDSASYLVSVITLRFMRVSFQVERAPAQRSLRRD
                     IAEGLRFLWQQPILRIMALLATSGNFLLSSVTLIVIVLAQGTLHLNVQVLGLIISAGG
                     IGGVLGGFVAPWIRARLRFGQVMIGSIFTWLLAIVALAIASSPLVLIAGVGLIGFTWL
                     VYGVTLVSYRLSLSPDHLQGRVNSAFRFLSYGAEPLGAAFFGLLLSLINPRLVLGLIA
                     AGLAVNGTVACLTRLRKA"
     gene            2433..4388
                     /locus_tag="VGT44_18820"
     CDS             2433..4388
                     /locus_tag="VGT44_18820"
                     /inference="COORDINATES: protein motif:HMM:NF025224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEV2582919.1"
                     /translation="MKQASLDRKSVGELQQRFSSQSKDVHEWGCQYNAEGGLISLNLS
                     DLGLSEVPSEIWKLTSLQELDMRSNQLAYLPAEFGQLTNLKKLSLQRNRLAHLPAELG
                     RLTHLQKLYLNDNQLEHLPVEIGQLACLEMLYLGNNCLAQLPGELGRLSKLRWLELGD
                     NQLTRLPGELGQLTSLQWLHLFNNRLTQLPAELGRLPNLQMILVKGNPDLRIPPPEAV
                     ARGPKAMLTYLRSLPPTPVERSEIKPPVAIEGEMLLPSQPPVSSVEQARIEMRYTMSF
                     FPTDLMSRMLERTQHYSQQARWTGGARLAYQGQQAQVELDPLQRQVRLIAWGPFPSTF
                     FTILKETLDLILSCYEGIAIKQELACPCAQQASIPHFYSYAELVKQLEKGGKSEITCP
                     ESGRRFSITSLLFGLHSSTTAQVVATIQQTRRGLSPTALKLDELIKRLDRIELGQQGI
                     LRVQLRSWNLQMQQLHKSCPGLFLLTRSQAFAINPRDWVSRPYRLHLLCQYPAGPHRI
                     EGEPGYEIRQGKEWWIDISPWLRCLVEVLQIGMPLGRSLGAIANQAMMNRFNNELNVF
                     QEILNDLLVFGAINAAQNGRIDLQAPPAEQQAQQAALRVLYHHLDKVDPTHRWQGLAQ
                     VVTSDGMILWLCDEHRKEFDPSSRGKQ"
     gene            4455..6143
                     /locus_tag="VGT44_18825"
     CDS             4455..6143
                     /locus_tag="VGT44_18825"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2582920.1"
                     /translation="MSLAYFCGKCGALQNSSDAFCRTCGASLAHPQTSSSTGQPYPGT
                     GRQSSLLTGQQFLGTRQQPSPSTGQPYPGTGQQPFPSTGQPYPGTGGQQPFPSTGQPY
                     PGTGRQPPGKILHGRYRLIQIIGQGGMGAVYIAHDLQLGNRLVALKEMSMSRLEPQEV
                     PRAIQQFQQEAYLLASLQHPNLPTIYEYFSEDGRWYLAMTYINGQSLQSCLDQAPGHK
                     FAVTEAVRIGIELCTVLNYLHTHTPQIIFRDLKPLNILMTSTGQIYLIDFGIARHFKA
                     DQTRDTSYYYSVGYAAPEQYGTSQTSARSDIYSLGATLHQMLSGLHPANRPFHFAPLN
                     MLDPTIPEPLAKLVAKMVEMDEESRPASVALVKQEMEKVLAPPPEPPQDVRTETETKP
                     PDKKKGLFGREKTRKSDGKKEAAPLHVAEKEPVRDEQVGTPQPSNQLPFPDQQGRKSE
                     TEKEQPSPLRDQILPSATSSDAALSPRGQNTGPSGTPTPQPSKKRAKRRLSPVVVAII
                     MIGIIALVIGVLYQGLVFGYHPARATAGIAGGVILLIIGLAWMLVTRSRSRAKR"
     gene            complement(6464..>7087)
                     /locus_tag="VGT44_18830"
     CDS             complement(6464..>7087)
                     /locus_tag="VGT44_18830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2582921.1"
                     /translation="PTGAGKSTIAGLMARFYEVVDGAILVDGYDIRDVTQHSLRSQIG
                     IVLQEPFLFTGTIRENIRYGRLDATNAEIEEAARAVGLHDLVAQLPDGYDTPIRERGR
                     NLSMGQRQLISFARALLADPRILILDEATANIDTFTELLVQQGLRRLLHGRTALVIAH
                     RLSTIKGADNIVVLQNGRIIEQGTHTELLQRDGAYASLYAMGFRHTA"
BASE COUNT         1547 a         2074 c         1987 g         1479 t
ORIGIN      
        1 attcatcggc ctatggagcg tatcgaacgg ctatctggcc gatgaatcgg cgatgcgcac
       61 ggtgaaccgg cgcctacgaa gtagtaatcc attctatatt taaaggagac aattactcat
      121 gtcgtggcaa ccacaacagg ggcagcctcc ccagagcgct ccacaacagc agcccgcagc
      181 cgatttgccc gaacacgcgc gccagcgttt gatgcagatg cgaggcgatg cgacgcggcg
      241 cggcctgttt accagcgatc tgtcggtcaa cgagtttttg ctgacgcgcg aagccggttt
      301 tgcgccgctc ggcctggtga tggggagttc catctaccat atcggcatca atatcgcgaa
      361 ctggaacaag aatcaggaaa tgcaggtgat gtctcaggcc atgtatcacg cgcgcaacct
      421 ggcgatgacc cgcatgcaag aagaggcgaa tgtgctgagc gcggatggcg tcatcggtgt
      481 caggctgatc gtcaatcgct accagtgggg cgaggctatc gcggagttta tggcgatcgg
      541 gacggctgta cgcgccttga gcggccagag ctttcgcaac gcgcgcggac tgccattcac
      601 atccgacctg agcggccagg atttctggac gttgctgcgc gccggttatc gaccggtcag
      661 catggtgatg ggcagttgcg tctaccacgt cgcccacatg gggctgcgcc aggccatgaa
      721 gacggtcggc cagaacatcg agatggtcaa ctacacgcag gcgctctatg atgcgcgcga
      781 gctggcgatg gaacgcatgc aggctgaagc gatgagtttt ggcgcgcagg gcgttgtcgg
      841 aatgcgcgtc caggaaggca gtcacggctg ggaatcgcac gtcatcgagt atttcgccgt
      901 cggcaccgcc gtgatcccaa tctcggaaga acaccaggtc ccgccgccga cgatgacgat
      961 ttccctgaac gatccttcat agccacaagg cgcatggatt caaaacagcc gcccaatgct
     1021 cgctcgttat ggcgcaacgg cgacttcctc ctgctgtgga gcgggcagag cgtgtccgtg
     1081 cttggcacaa acatctcagc cctggcgttg cccctgctcg tgctggccgt gacgcactcg
     1141 ccagcgcagg ccgggttgat cgcggcagtg caacagttgc cctacctgct cttcagcctt
     1201 cccgctggcg ccctgctcga ccgctgggat cgcaagaaga ccatgattcg ctgcgacctg
     1261 ctgcgctgga tcgccctggg ttctgtgcca ctggcctatg ccttcggcca tctcacgctc
     1321 gcgcagttct acatcgtggc ctttgtcgat ggctccgcct ttgtcttttt cagcatcgcg
     1381 cagatcgccg cgctgcccca ggtcgtcgcg aaggatcacc tgccacgcgc atattccctg
     1441 acgaccatgt cggagtacgc cgggcaactg cttggtccga gcctggcggg cttcatcatc
     1501 ggcctcgcgc ccgtggtcgt cgcgggtgca atcatggtgt acctgctcga tagcgcgtcc
     1561 tatctcgtct cggttatcac gttgcgcttc atgcgcgtct cgtttcaggt ggaacgcgcg
     1621 ccagcgcagc gctcgctgcg cagagacatc gccgaggggc tgcgctttct ctggcagcag
     1681 cctatcttgc gcatcatggc tcttctggcc acgagcggca atttcctgct cagctcggtg
     1741 acgctaatag taattgtgct ggcgcaaggc acgctgcatc taaacgtgca ggtgttaggc
     1801 ctcatcatct cggcaggcgg catcggcggc gtgctgggcg gctttgtcgc gccctggatt
     1861 cgcgcgcgac tgcgttttgg gcaggtcatg atcggctcga tcttcacctg gctgctggcg
     1921 atcgtcgcgc tggccatcgc atcttctccg ctcgtgctga tcgccggggt tggcctgatc
     1981 ggtttcacct ggctggtcta tggcgtcacc ctggtgtcct accgcctctc gctctcgccc
     2041 gatcacctgc aaggccgcgt caacagcgcc ttccgcttcc tctcctacgg cgcggagccg
     2101 ctcggcgcag ccttcttcgg cctgctgctc tcgctaatca acccccgcct tgtgctggga
     2161 ctgatcgcgg cagggttggc ggtgaatggc acggtggcct gcctgacccg attaagaaaa
     2221 gcttagatga tgttttctgt gttcatggtc ggttatgaac ttttataaat gaaactggac
     2281 acgtgttttt gtgtaaatcg tgaaagcaaa gttagctata ttcccattgt tcgttttatc
     2341 tatcaagaag ctcatccctt tcttaataaa aagggactat gagtacggca cgcaccaaag
     2401 gtggaagaga gagactagac aaggcgtaat cgatgaaaca ggcatcactc gataggaaga
     2461 gtgtggggga gcttcaacag cgattctcaa gccagagcaa agatgtgcat gagtggggtt
     2521 gtcagtataa tgcggaagga ggtctcatca gcctcaattt gagtgacctg ggtctctctg
     2581 aagttccgtc tgaaatctgg aaactgacct ctctacaaga actggacatg cggagtaatc
     2641 aactggcata tctacccgcg gaatttgggc aacttaccaa cctgaaaaag ttgtccttac
     2701 aacgcaatcg cctggcgcac ctgcccgcgg aactgggccg gctcactcac ctgcaaaaat
     2761 tatacctgaa cgataaccag cttgagcacc ttcccgtaga aattggccag ctcgcgtgcc
     2821 tggaaatgct ctacctgggc aacaattgcc tcgcccaact gccgggagaa ttggggcgac
     2881 tgagcaagct gcgctggttg gaactaggcg acaatcaact gacccgactt ccgggggagt
     2941 tgggccaact caccagcttg caatggctgc acctgttcaa caaccggctg acgcagcttc
     3001 ctgcggaact ggggcggctt cccaacctgc aaatgatttt ggtgaaaggc aatcctgatc
     3061 ttcgcattcc tccgccggag gcggtggcgc gcggcccgaa agcaatgctg acctacttgc
     3121 gcagccttcc cccaacacca gttgagcgat cggagataaa accaccggtg gcaatcgagg
     3181 gagagatgct gcttccatcg caaccgcctg tttcgtcggt tgaacaggcg cgcatcgaaa
     3241 tgcgatacac gatgagcttt ttccctacag acctgatgag ccgcatgctg gagcgcacgc
     3301 agcactacag tcagcaagcg cgctggacag gcggcgcgcg gctggcctac cagggtcagc
     3361 aggcacaggt ggaactcgac cccctgcagc gccaggtacg tctgatcgcc tggggaccat
     3421 ttccctccac cttttttacg atcctgaaag agacgctgga tctcatactg agttgctatg
     3481 aaggaattgc catcaagcag gaactcgcct gcccctgcgc tcagcaggca tctatccctc
     3541 acttttattc gtatgccgaa ctggtgaaac aattggagaa gggtggtaag tccgagatca
     3601 cctgtccgga gagtggaagg cgcttttcca taaccagcct gctctttggt ctgcattcca
     3661 gcacgacggc gcaggtggtg gcgactattc agcagacgcg gcgagggctt tctccaaccg
     3721 ctctgaagct cgacgagctg atcaaacgcc tggatcgcat cgagctgggc cagcagggca
     3781 tcttgcgcgt gcagctccgt tcctggaacc tgcagatgca gcagttgcat aaatcatgcc
     3841 ccggcctctt tcttctcaca cgaagtcagg ccttcgcgat caatcccaga gactgggtaa
     3901 gccgccccta tcgtctccac ctgttgtgcc agtatcctgc aggtccacac cgcatcgaag
     3961 gcgaacccgg ctacgaaatc cgccagggga aagagtggtg gattgatatc agtccctggc
     4021 ttcgctgcct ggtggaggta ctccagattg ggatgcccct gggcagatca cttggagcga
     4081 tcgccaacca ggcgatgatg aatcgtttca acaatgagct aaatgtgttc caggagattt
     4141 tgaacgacct gctcgtcttc ggagcaatca atgcagccca gaatggccgc atcgatctgc
     4201 aggcgccgcc tgctgaacag caagctcaac aggctgctct gcgggtactc taccatcatt
     4261 tagacaaggt cgatccaacc caccggtggc agggacttgc ccaggtcgta acatccgatg
     4321 gcatgatctt atggctttgt gatgagcaca ggaaggagtt tgatccgtca tcccggggaa
     4381 aacaataggc aaacagggac agaagaagaa ccggtccgct ttcgtcaaaa tagcaattgt
     4441 aaggagagaa gcaaatgagt ctcgcgtatt tctgtggcaa atgcggcgca ctacagaact
     4501 cgagcgatgc gttctgtaga acatgcggcg cgtctttagc ccatccacag acatcgtcct
     4561 caaccgggca gccatatccg ggaaccgggc ggcagtcatc acttttaacc gggcagcaat
     4621 ttctaggtac aaggcaacag ccatcaccct caaccgggca gccatatccg ggaaccgggc
     4681 agcaaccatt cccttcaacc gggcagccat atccaggaac tggaggacag cagccattcc
     4741 cttcaaccgg gcagccatat ccaggaaccg ggcggcagcc gcctgggaaa atcttacatg
     4801 ggcgctatcg cctgatccag ataatcgggc aaggagggat gggcgccgtc tatatagccc
     4861 acgacctgca gttgggcaac cggctagtcg ctctcaaaga gatgagcatg agcaggctcg
     4921 agcctcaaga agtaccgcgc gccattcagc agtttcaaca ggaggcgtac ctgctggcca
     4981 gcctgcagca ccccaacctg cctaccatct acgagtattt tagcgaggat gggcgctggt
     5041 acctggccat gacttatatc aacggccaga gcctgcaatc ctgtctcgat caggctcccg
     5101 gacataagtt tgccgtcacc gaggcggtgc gcatcggaat cgagttgtgt acggtactca
     5161 actacctcca cacccacacg ccgcagatta tcttccgcga cctgaagccg ctcaacattc
     5221 tcatgacctc tacaggccag atttacctga tcgattttgg aattgcgcgc catttcaaag
     5281 cggaccagac ccgcgataca tcctactact actctgtcgg ctatgccgct cccgaacaat
     5341 acggcacgag ccagacgagt gcgcgctcgg atatctacag cctgggcgca acgctccacc
     5401 agatgctctc agggttgcac ccggccaaca ggcccttcca tttcgcgccc ctgaacatgc
     5461 ttgacccaac aattcctgaa ccactggcaa aactagtggc caaaatggtg gaaatggacg
     5521 aagagagccg gcccgccagt gtagcgctgg tcaaacaaga gatggaaaaa gtcctggctc
     5581 ctccacccga gccacctcag gatgttcgta cagagacaga gacgaaaccg cccgataaga
     5641 aaaagggcct tttcggaaga gaaaaaacca ggaagtcgga tggaaaaaaa gaggcggcac
     5701 cgcttcacgt cgccgaaaag gagccggtgc gtgatgaaca agttggtaca ccacaaccga
     5761 gtaatcagtt gcccttcccc gatcagcaag gacgcaaaag tgagactgag aaggaacagc
     5821 catctccgtt gagggaccaa atattgccgt cagccacctc ttcagatgcg gctctgtctc
     5881 cccggggcca gaacacaggc ccttcaggca cgcccacacc tcaaccatcc aaaaagcggg
     5941 ctaaacgtcg gctctcacca gttgtcgtcg ccattattat gattggtatt attgcactgg
     6001 tgataggcgt gttataccag ggactggtct ttggctacca tcccgcgcgc gctactgccg
     6061 gtattgcggg gggagtgatc ttgcttatta ttggccttgc ctggatgttg gtgacgcgga
     6121 gcaggagtag agcaaaaaga tgaaccctgc ttgctcaatg ctatgttttt ggcatggatg
     6181 ccgactagca gtgcaactcg aatacgagcg cgaaccaagc tgcgggatct cgggtatcac
     6241 aggtaactgt gaccgtgagt ttttacctgt tctggagaag tgtacggcta taacggcgcg
     6301 ctgactctgg cacaaaactt gctgaaacag catatgcgac agcaatatcc cacctatatg
     6361 ttggtgggca atatttcagc aatcatccag gcggcaacga tgatatactt ccaaccaatc
     6421 cttctcagat catctttaca atatcaggag gctgaccacg cccttacgcc gtatgcctga
     6481 accccatcgc atacagagag gcatacgcgc cgtcgcgctg caacagttca gtgtgtgttc
     6541 cctgctcgat gatgcggcca ttctgcagaa caacgatatt gtcggctcct ttgatagtcg
     6601 agaggcggtg cgcgatgacc agcgccgtgc ggccatgcag cagacgccgc agtccctgct
     6661 gcaccagcag ttcggtgaag gtgtcgatat tggcggttgc ctcgtccagg atcaggatgc
     6721 gcgggtcggc cagcagggcg cgcgcgaacg agatcaactg gcgttgtccc atgctcaggt
     6781 tgcgcccgcg ctcgcggatt ggcgtgtcat agccatcggg caactgcgcc actaggtcgt
     6841 gcagacccac tgcgcgcgcg gcctcttcta tctctgcgtt ggtggcatca agccggccat
     6901 agcggatatt ctcgcggatc gtaccggtga acaggaacgg ctcctgcaaa acgatgccga
     6961 tctggctgcg cagtgaatgc tgggtcacat cgcgaatatc gtagccatcg accagaatgg
     7021 ctccgtccac tacctcgtag aagcgtgcca tcagcccggc gatggtgctt ttcccggcgc
     7081 ctgtcgg
//
LOCUS       DASZAK010001065         2832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_633072, whole genome shotgun sequence.
ACCESSION   DASZAK010001065 DASZAK010000000
VERSION     DASZAK010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_633072"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VGT44_18835"
     CDS             complement(<1..749)
                     /locus_tag="VGT44_18835"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV2582922.1"
                     /translation="MTTNVILPALGMAQENGTIVRWLKDEGEQVTKGEPIAEIETDKA
                     TVEIEAPASGQLVSITAAAGDVVPTGQVIARIVSADENKRSASPLASRIAAEHQLDVS
                     LVPSTGRRIQKADVVSYLQSQQRPAQAVSPGQERAPRLLPASPKARRLAREYGANLAA
                     IKGTGPEGAVLAADVARVGAPPSAATVPVAAAQAASPAEMNGAAFEAGEMEQAGETVS
                     ESASELAVGTIWRVMAERTAQSWSGTPHFYLV"
     gene            complement(762..2120)
                     /locus_tag="VGT44_18840"
     CDS             complement(762..2120)
                     /locus_tag="VGT44_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEV2582923.1"
                     /translation="MAIKTYGLMGVDWEQRVNFERLRTERLARIKRLLKQSSMGALLC
                     FDMTNVRYITATHIGTWAMDKLARFTLLPQDDEPILWDFGSAARHHQLYCPWLGERSR
                     AGISTLRGAMLPEAGRAEDVARKIRIELEMRGLLNEPVGIDAVELPVLFALQAEGIKV
                     VDGQQLMQQARLIKTQDEITLLASACMMVDAAYDELYRFMKPGVRENECVGLVSKVLY
                     DLGSEHVEGVNAISGERCSPHPHVFSDRALRPGDPAYFDILHSYNGYRTCYYRTFAVG
                     SASQSMVDAYKRCRDILDVAIAGIRPGVTTTDIVKLWPRAEEFGFPNEEAAFALQYGH
                     GVGLSIWEKPIFSRLVSLDHPEVIEEGMVFALETFWPASDGWTAARIEEQLVVTKDGC
                     EVITRFPAEELLVAGVRYYTTGGPLPTTRETQSNLNTQAGTQNGHQSGWATVSEGDRK
                     GI"
     gene            complement(2124..>2832)
                     /locus_tag="VGT44_18845"
     CDS             complement(2124..>2832)
                     /locus_tag="VGT44_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2582924.1"
                     /translation="DQIVNQAAKVHYMSGGKLKVPLVIRTTLGATRRTAAQHSQSLHA
                     WVSHIPGLKVALPSTPYDAKGLMKTAIRDDNPVIFFEDKMMYQLKGMIPEGDYTIPFG
                     VADIKRAGTDLTIVATSSMVQVALAAAETLATLDISVEVVDPRTTAPLDTQTLIESAK
                     KTSRAIVMDEGYERYGVTAEIAAAIAEGAFYYLDAPVKRMGAMDVPVPFSPVLEDETV
                     PTPTTVVEMAKTLCGRN"
BASE COUNT          479 a          923 c          864 g          566 t
ORIGIN      
        1 accaggtaga aatgcggcgt cccgctccag ctctgcgcgg tgcgctcggc catcacccgc
       61 cagatcgtgc cgacagccag ctcgcttgcc gattcgctca ctgtttcacc cgcctgctcc
      121 atctcacctg cctcgaacgc cgcgccgttc atctcagcag gagacgctgc ctgcgccgcc
      181 gctactggaa cggtagccgc gcttggcggc gcgcctaccc gcgcaacatc ggcagccagt
      241 accgcgccct ccggccctgt ccccttgatg gcagccaggt ttgcgccgta ttcacgcgcc
      301 agccgccgcg ccttcggaga agcgggcagc aggcgaggag cgcgctcctg gccggggctg
      361 acagcctgtg ctggccgctg ctggctctgc aaatacgaca ccacatcggc cttctggatg
      421 cgccgcccag tgcttggtac gaggctcaca tcgagctggt gttcggcggc aatgcgcgac
      481 gccagtggtg aggcgcttcg cttgttttca tctgccgaga cgatgcgcgc gatcacctgc
      541 cccgtaggga cgacgtcacc ggcagcagcg gtgatggaga cgagctggcc tgaagcaggc
      601 gcctcgatct cgacggttgc cttgtcggtt tcgatttctg cgattggctc gcccttcgtc
      661 acctgctcgc cctcgtcttt gagccagcgc acgatggtac cattttcttg agccatacct
      721 aaggccggta gtatgacatt cgtcgtcatc ggtgatcctt tttatatccc cttgcggtcg
      781 ccctcagata ccgttgccca gccggactga tgcccgttct gcgtccccgc ctgcgtgttc
      841 agattcgact gcgtttcgcg cgtggtgggc agcgggccgc cggtcgtata gtagcgcaca
      901 cccgccacga gcagttcctc ggcgggaaaa cgggtgatga cttcgcagcc gtccttcgtg
      961 acgacgagct gctcttcgat gcgcgccgcc gtccagccgt ccgaggctgg ccagaaggtt
     1021 tcgagggcga agaccatgcc ctcttcgatc acctccgggt gatcgagcga gaccaggcgg
     1081 ctgaagatcg gcttctccca gatcgagagg cccacgccat gcccatactg gagcgcgaaa
     1141 gcggcttctt cgttggggaa gccaaactct tcggctctgg gccagagctt gacgatatcg
     1201 gtcgtcgtca cccccggcct gatgccggca atagccacgt ccaggatatc gcgacagcgt
     1261 ttgtaggcat cgaccatcga ctgcgacgcg ctgccaacgg caaaggtgcg gtagtagcag
     1321 gtgcgatagc cgttatagct gtgcaggatg tcgaaatagg ccgggtcgcc gggacgcagt
     1381 gcgcggtcgc tgaagacgtg cgggtgcggg ctgcagcgct cgccggaaat ggcgttgacc
     1441 ccctcgacat gctcggagcc gagatcgtaa agcaccttgc tgacgagacc cacgcactcg
     1501 ttctcgcgca caccgggctt catgaagcga tacagctcat cgtaggcggc gtccaccatc
     1561 atacaggcgg aggccagcag cgtgatttcg tcctgcgttt taatcaggcg cgcttgctgc
     1621 atcaattgct gcccatcgac gaccttgatg ccctcggcct gcagcgcgaa gagtaccggc
     1681 aactcgaccg cgtcgatgcc gaccggctcg ttcagcagcc cgcgcatctc cagctcgatg
     1741 cgaatcttgc gcgccacgtc ttccgcgcgc ccggcttccg gcagcatcgc gccgcgcagg
     1801 gtagagatcc cggcgcgcga acgctcgccc agccagggac agtacaattg atgatggcgc
     1861 gccgccgagc cgaagtccca caggatcggc tcgtcatcct ggggaagcag cgtgaatcgc
     1921 gccagcttat ccatcgccca cgtgccgata tgcgtcgcgg tgatatagcg cacgttcgtc
     1981 atgtcgaagc acaacagcgc ccccatcgac gattgcttga ggagccgttt gatgcgggcc
     2041 aggcgctcgg tgcgcaaccg ttcaaagttg acccgctgct cccagtccac acccatcagt
     2101 ccatatgttt ttatcgccat agttcaattc ctcccgcaca gcgttttcgc catctccacg
     2161 acggtcgtgg gggtgggtac ggtttcatct tccaggacgg gtgaaaaagg caccgggaca
     2221 tccatcgcgc ccatgcgctt gacgggcgca tccaggtagt agaacgcgcc ctcggcgatg
     2281 gcggcagcga tctccgccgt gacgccgtag cgttcgtagc cttcgtccat tactatcgcg
     2341 cggctggtct ttttcgccga ttcgatcagc gtctgcgtat cgagcggcgc ggtcgtgcgc
     2401 gggtcaacca cctcgacgct gatgtccagc gtcgccagcg tctcggcagc ggcgagcgcg
     2461 acctgcacca tactgctggt ggccacaatc gtcaggtcag ttccggcgcg tttgatgtcg
     2521 gcgacgccga aggggatggt atagtcacct tcggggatca tgcccttcaa ctggtacatc
     2581 attttgtctt caaagaaaat gaccgggtta tcgtcgcgaa tggcggtttt catcagccct
     2641 ttagcgtcgt atggcgtcga aggcagcgcg actttcaaac cggggatatg gctgacccag
     2701 gcgtgcaggc tctggctatg ctgcgcggct gtgcggcgcg tggcgcccag ggtcgtgcga
     2761 atcaccaggg gaaccttcag cttgccgccc gacatgtaat gcactttggc ggcctggttg
     2821 acgatctggt cc
//
LOCUS       DASZAK010001066         4033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_525954, whole genome shotgun sequence.
ACCESSION   DASZAK010001066 DASZAK010000000
VERSION     DASZAK010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525954"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VGT44_18850"
     CDS             <1..1274
                     /locus_tag="VGT44_18850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEV2582925.1"
                     /translation="YDLLAGRLNVKRHHHLSRARVQKLAPTLVTKGLKEGFVYYDAQT
                     HDARLTMALIRTAAQYGATIANYAEVTSFVIEKDKAIGAHVHDRLNDQQLTVYARHIV
                     NATGVYSEEIEALTGDTPQVQIEPSKGVHLVLSREDLKLGDSAIVLPETDDRRILFIV
                     PWETRAIYGTTDTGSGDLNHPAANRDDIAYLLEHLNRYLSISLTEEDIISTYAGYRPL
                     LKPRQNSEHSSAKLSRTHAVLQSHAGIVTIVGGKLTTYRRMAQDTIDVLNKRDGSAVV
                     HPTLNMPLHGSAGWPAVKRDLQTKGKALGLSEETVTHLGKSYGAESLAVLHLVESDSS
                     LAELLISDLPYIKAEVIYACRNEMAMTPDDMLARRTSITLLDRQRGMGIVDDVAALMA
                     PELGWSPVEQQNVIDAFNAAMHDQMVAEKSV"
     gene            1409..2410
                     /gene="dhaK"
                     /locus_tag="VGT44_18855"
     CDS             1409..2410
                     /gene="dhaK"
                     /locus_tag="VGT44_18855"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEV2582926.1"
                     /translation="MKKLINQVENVVKEALMGMAAAHPDLIDVDLEQQIVVRANAPVQ
                     GKVGVISGGGSGHEPMHGGFVGYGMLDAACPGAVFTSPVPDQMLAATKAVNGGVGVLH
                     IVKNYTGDVLNFEMAAELAAADGIEVAAVVTNDDVAVQDSLYTAGRRGVGVTVLLEKI
                     VGGLAETGAPLTQVADLARKVNSQGRSMGMALTSCIVPAAGKPTFDLGDDEMEIGIGI
                     HGEPGRRRVKLASADDITEMLATPVIEDLGLKAGDQVLAMVNGMGGTPLIELYIVYNA
                     LNRILGGRNITIARNLVGNYITSLEMAGCSITLVRLDNELTQYWDAPVHTPALRWGM"
     gene            2618..3244
                     /gene="dhaL"
                     /locus_tag="VGT44_18860"
     CDS             2618..3244
                     /gene="dhaL"
                     /locus_tag="VGT44_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HEV2582927.1"
                     /translation="MTNEDVKRWLERVADVLHENAGYLTQLDSAIGDADHGINMDRGF
                     KAVREKIAAIPTPDIATLLRTVGTTLVSTVGGASGPLYGTAFLRAAGAVVGKEELAAA
                     DFVAMLEAFLGGIVARGKAQPGEKTMVDALTPALAAAKEALNAGASLAETTRRAADAA
                     EAGMKATIPMLATKGRASYLGERSIGHQDPGATSSSLMLRTLTETAAS"
     gene            3376..>4033
                     /gene="dhaM"
                     /locus_tag="VGT44_18865"
     CDS             3376..>4033
                     /gene="dhaM"
                     /locus_tag="VGT44_18865"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HEV2582928.1"
                     /translation="MTVGVVIVSHSAQLARGVAELAGQMAQGKARIEPAGGAIDGSLG
                     TSADTILAAIQSLDGPDGVLVLLDLGSALLSAEVALEMLNDEQRAHTRLTYAPLVEGA
                     VAAAIEATLGRSLAEVQQAAEKTAGTAQLQLLKPISQPEHEPVPASTPTLPLPTPNEI
                     EAKLLLTNPTGLHARPASLFVQTAAKFQATIQAQVEGRARQANAASIMEVLSLGARNG
                     D"
BASE COUNT          885 a         1251 c         1165 g          732 t
ORIGIN      
        1 tgtatgatct gctcgccggg cggcttaacg tcaaacgcca tcaccatctg agccgcgcgc
       61 gcgtgcagaa acttgcgcca acgctggtga cgaaagggct gaaagagggc tttgtgtact
      121 acgatgcgca gacccacgat gcccgtctca ccatggcgct cattcgcacg gcagcccagt
      181 acggcgcgac tatcgccaac tacgcggaag tgacctcgtt cgtcatcgaa aaggacaaag
      241 cgattggtgc gcacgtgcat gatcgcctca acgaccagca actgacggtc tatgcccgcc
      301 acatcgtcaa cgccaccggt gtctactccg aagaaatcga agcgctgacc ggtgacacgc
      361 cccaggtaca gatcgaacca agcaagggtg tccacctggt tctctcacgc gaagacctga
      421 agttgggtga ttccgctatc gtcttgcccg aaaccgatga ccggcgcatt ctctttattg
      481 tcccctggga gacacgcgcg atttacggca ccaccgacac gggttcgggc gacctcaacc
      541 acccggcggc aaaccgcgac gacatcgcgt atctcctcga acatctcaac cgctatcttt
      601 ccatttccct caccgaagag gatatcatca gcacctatgc cggctatcga cccttgttga
      661 aaccccggca gaactctgaa cactcgtcgg cgaaactgtc gcgcacacac gccgttttgc
      721 aaagccacgc cggaatcgtg acaattgtcg gaggaaaact gacgacctac cggcgtatgg
      781 ctcaagatac gatcgatgta ctgaacaaac gcgatggttc cgcggtcgtc catcccacgc
      841 tcaatatgcc gctgcatggc agcgcgggct ggcccgccgt gaagcgcgac ctgcaaacaa
      901 aaggcaaagc actcggtctg agtgaagaga ccgtaactca cctggggaaa agctacgggg
      961 cagagagcct cgcggtgcta cacctggtcg aaagcgactc ttctctcgcc gaactgctga
     1021 ttagcgacct gccatatatc aaagcagaag tcatctatgc ctgtcgtaac gagatggcga
     1081 tgactcccga cgatatgctg gcgcgccgta cctcgatcac cttgctggac cgtcagcgtg
     1141 gcatgggcat tgtcgatgac gttgcagcac tcatggcccc tgagcttggc tggtcgccgg
     1201 tggagcaaca aaacgtcatc gacgccttca acgctgccat gcacgaccag atggttgccg
     1261 agaagagtgt ttgacaggag gtgtagcatg gcgtctgcaa gagtcgccac tacatgacat
     1321 aataccatca tggcgagtca ttcgaaatgt agtggcgacc ctcgtggtcg ccatgcaacc
     1381 ttacgtagga attcaagaag gaggtagcat gaaaaaactc atcaaccagg tagaaaatgt
     1441 cgtcaaagaa gcattgatgg gcatggcggc tgctcaccct gacctcatcg acgtcgatct
     1501 tgagcagcag attgtggtgc gcgcgaacgc cccggtgcag gggaaagttg gggttatttc
     1561 cggcggcggc agcggccacg agccgatgca cggcggcttt gtcgggtatg gaatgcttga
     1621 tgcagcctgc cccggcgcgg tcttcacctc gcccgttccc gaccagatgc ttgccgcgac
     1681 gaaagccgtc aatgggggag ttggcgtact gcacatcgtc aagaactaca ccggcgacgt
     1741 gctgaacttc gagatggcgg cggaactggc cgccgccgat ggcattgaag tggccgccgt
     1801 cgtcaccaac gacgacgtag ccgtgcagga cagcctctac acggctggtc ggcgcggcgt
     1861 cggcgtgacc gtgctgctcg aaaagattgt cggcggcctg gccgagacgg gtgcgccact
     1921 gacccaggtc gccgatcttg ctcgcaaggt caacagccag ggtcgcagca tgggcatggc
     1981 cctcacctcc tgtatcgtgc cggcagcagg caaacccacc tttgatctcg gcgacgacga
     2041 gatggagatc ggcatcggca ttcacggcga gccgggccgc cgccgcgtca aactcgcctc
     2101 ggctgacgac atcaccgaga tgctggctac tcccgtcatc gaagacctcg gcctcaaagc
     2161 aggcgaccag gtactggcga tggtcaacgg catgggcggc actccgctca tcgaactcta
     2221 catcgtttac aacgccctga atcgcatcct gggcggcaga aacatcacca tcgcgcgcaa
     2281 cctggtcggc aactacatca cctcgctcga gatggccggt tgctcgatca cccttgtgcg
     2341 cctggataac gaattgacgc aatactggga tgcccccgtg catacccccg cgctgcgctg
     2401 gggcatgtaa cttttcgcca ccatttttgg gtgacatcat ctgtaggtgc cgattgatcg
     2461 gcaacagcct gtcatcctga gcgcagcgaa ggatctcggt cctcgcgcat cagatccttc
     2521 gctgcgctca ggatgacaac ttactaccga tctcgctcgt aaaagttatc aatcggctcc
     2581 tatcaccttg atagtatttg aacagaaagc ctgaattatg accaacgaag atgtcaaacg
     2641 ctggctagaa cgtgtcgctg acgtgctgca tgaaaacgcc gggtacctga cgcaactgga
     2701 ctccgctatc ggtgacgcgg atcacggcat caacatggat cgcggcttca aggcagtacg
     2761 cgagaagatt gcagctatac caacacccga tatcgccacg ctgctgcgta cggttggcac
     2821 cacgctggtt tcaacggtag gcggtgcaag cggtccgctc tatgggacgg ccttcctgcg
     2881 cgccgccggg gccgtggtag gaaaggaaga actcgccgcc gccgatttcg tcgcgatgct
     2941 cgaggcattc ctcggcggca tcgtcgcgcg cggcaaggcc cagcccggag agaaaacgat
     3001 ggtggacgcc ctcacgcctg ccctggccgc tgcgaaagag gcgcttaacg caggcgcgag
     3061 cctggccgag accacacggc gtgcagctga tgctgccgag gcaggcatga aggccactat
     3121 ccccatgctg gcaaccaaag ggcgtgcgag ctatctgggc gaacggagca tcggacacca
     3181 ggaccccggc gcaacttcga gttcgttgat gctgcgaacc ctgaccgaaa ccgctgcatc
     3241 gtaggcaggt ggatgaaccc gttgtagggg cgcaattcat tgcgccctgg ttcgctcctt
     3301 gcatcagcct ggcacgaggg cgcaatgaat tgcgccccta caacgggttc atcagaaaag
     3361 aagaagaaat agactatgac ggttggggtt gtcatcgttt cccatagcgc ccaactggca
     3421 aggggtgttg ccgagcttgc cgggcagatg gcccagggca aagctcgcat tgagccagca
     3481 ggaggtgcca tcgatggaag tttaggcacc tcggctgata ccatccttgc cgcgattcaa
     3541 tcgctggacg ggccagatgg cgtcctcgtg ctgctcgacc tgggcagcgc gctgctttct
     3601 gccgaggtcg cgctggagat gctgaacgac gagcagcgcg cccacacgcg cctgacctac
     3661 gcgccgcttg tcgagggcgc agtcgctgcc gctatcgaag ccacgcttgg ccgctcgctt
     3721 gcggaggtac agcaagcggc ggagaagacc gccggaacag cacagttgca actgctcaaa
     3781 cccattagcc agccggagca tgagcctgtt cccgctagca caccaaccct gccacttccc
     3841 acacccaacg agatcgaggc gaagctgctg ttaaccaacc cgaccggttt acacgcccgc
     3901 cctgccagtc tcttcgtaca aaccgccgcc aaatttcaag ccaccatcca ggcgcaggtc
     3961 gagggcagag cgcggcaagc caacgccgcc agcatcatgg aagtcctctc gctcggcgcg
     4021 cgcaacggcg aca
//
LOCUS       DASZAK010001067         3816 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_132514, whole genome shotgun sequence.
ACCESSION   DASZAK010001067 DASZAK010000000
VERSION     DASZAK010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132514"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VGT44_18870"
     CDS             complement(<1..553)
                     /locus_tag="VGT44_18870"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2582929.1"
                     /translation="MTDIPAVVSSGSTPSIGPQARLQMELFRPPLHYPVHPYAELLAH
                     TAEKYPENEAMLTRDVTLTYRELNALVNMFANALLTQGIQKGQKVCLFMMNRAEYMIS
                     WVAAARIGAVVSQMNPSYKEREVAYQMHNCEAAAVIVQYELLPLVQAVRAEVPSLRQV
                     IVIGAGGRSLPVDVISYSRILQAQ"
     gene            756..1031
                     /locus_tag="VGT44_18875"
     CDS             756..1031
                     /locus_tag="VGT44_18875"
                     /inference="COORDINATES: protein motif:HMM:NF017793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HEV2582930.1"
                     /translation="MSGTIHVNTELMRDLGRRFQLNCQYAQEKIIDELRAMSGQMEGD
                     WVGVSRIHYDELFQHWCQSAQSLITWGQEIGVHLDKTANYFEQADNS"
     gene            1296..2132
                     /locus_tag="VGT44_18880"
     CDS             1296..2132
                     /locus_tag="VGT44_18880"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2582931.1"
                     /translation="MHTILEDFSANALLNAMEVNLQEAWVRFGRWIGANVHEEPGLFW
                     FDSAYSFQLANGIVRTQFPLDGQEQKLDARIQQLTAEHVAMACMVGPSTRPADLGNRL
                     EAHGWVLDAAPGMAVDLQTLSEQPRPTSPLTIERVHDTEMLKTWLRVMMIGSEIPEAG
                     LNILLDIVSQQGYKDSPSAHYYLGWLDGVPVATSLLFLAGGVAGIYNVATLLAFRGKG
                     IGSALTVVPLLDARALGYRIGVLQSSPMGFNLYKRLGFKQYCTFYAYFWQAANLDSSE
                     KE"
     gene            2230..3813
                     /locus_tag="VGT44_18885"
     CDS             2230..3813
                     /locus_tag="VGT44_18885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2582932.1"
                     /translation="MTTLDNSATPANAVPASQRPPHYLDVHALMRPDKPALICEDRIL
                     TYAEFNARARRVANVLSMLGVQAGDRVGVIAHNSIELLEVAGGLSKLSAIGVLLSFRL
                     REQEIAYILNDSQAKVAIAGPGLASVVNLARPSVDHKIAFIAIGDDEIGESDWLLYEA
                     LLTGASAEIPESESGLGSTMSYTSGTTGKPKGAYRPRGVSPLHILRLVQIFGLSETDV
                     HLLAGPYYHSAPNFFTAAHLLLGATVVIQPKYDPADALQLIERHHITTTFMAPTLLHR
                     LCDVPQDEFARYDVSSLHSIILGGAPCAYALKVRAIERFGEIVWEFYGATETGFVTIL
                     RPEDQLRKPGSCGTVGPGQEILLLDAEGNEVPDNVPGEMWARNDFLAEYYNKPEATAS
                     NTKDGFFSVGDIAYRDAEGYYFICDRKIDMVISGGVNIYPAEIEAVLTAHPAIADVAV
                     IGVPDEKWGESVMAVVELLPGVSATAQELIEFCSARLADYKRPRSVDFVDQLPRNPAG
                     KLLKTRIREPYWKDVGRRI"
BASE COUNT          870 a         1042 c         1023 g          881 t
ORIGIN      
        1 attgcgcctg taggatgcga ctatatgaaa tgacatcgac aggcagcgat cgtccgcccg
       61 cgccaatgac gatcacctgc cggagcgagg gtacttccgc ccgaaccgcc tgcaccagtg
      121 gcagcaattc atactgtaca atgaccgccg ccgcttcgca gttatgcatc tgatatgcta
      181 cttcgcgctc cttatatgat ggattcattt ggctgaccac cgctccgata cgcgccgcag
      241 cgacccagct aatcatgtat tccgcccggt tcatcatgaa caggcagacc ttctgaccct
      301 tctgtatgcc ttgagtaagc agcgcgttcg caaacatatt gaccagcgca ttcaattctc
      361 tataggtcag cgtgacatcc ctggtcaaca tcgcttcgtt ttcagggtac ttttcggcag
      421 tgtgcgccag caattcagcg tatggatgaa ccggatagtg taaaggcggg cgaaataact
      481 ccatttgtaa gcgcgcctga gggccaatcg agggcgtcga cccagacgac acaacagcag
      541 gtatatcggt catgcgcttc tctccaatca aatttgaaag gtcagaatac cggcttgatt
      601 tcctacaaca ttagtatcgt tgaaagatcg taatgtcaag gcaaccgttc ggttatgcgg
      661 aaagcatccg catttggtga cttcttttat actacaaata agcggcgagc aagccgcatc
      721 cgaaaggcaa tttatttttg attggagtac acttcatgag cggtacaatt catgtcaata
      781 cggaattgat gcgcgatctt ggcagacgct tccaactgaa ctgccaatac gcgcaggaaa
      841 aaatcatcga cgaacttcgt gccatgtcgg gacagatgga aggcgactgg gttggcgtca
      901 gccgcatcca ttatgacgag ctgttccagc actggtgtca gagcgctcag agcctgatta
      961 cctggggtca ggaaatcggt gttcacctcg acaaaacggc caactacttc gagcaggccg
     1021 acaattcata gccaccgttc tcctttcctt ccccaataga gcctcgagaa aatatctctc
     1081 gaggctcttt tcgctgccct taaactatag tatagccgca aagttcatac gccatattct
     1141 tctagagtta tgtggaacgt cttttaatat atactgtatt tatgtataat agttgacaaa
     1201 tacgttattt tgtgctatcc tttcttcgcg tgagcatccg aaatcccgta ggagggctaa
     1261 tagtcaacaa ttacctttag aaaggataac ataccatgca cacaatcctg gaagatttct
     1321 cggctaacgc actactaaat gctatggagg ttaatctcca ggaagcgtgg gtgcgctttg
     1381 ggcgctggat tggggcaaac gtacacgaag aacctggatt gttctggttc gactcagcgt
     1441 attcatttca gcttgccaat ggcattgtca gaacgcaatt tcccctggat ggtcaggagc
     1501 aaaagcttga cgcgcgcatc cagcagctta ctgctgagca tgtcgcgatg gcctgtatgg
     1561 tcggcccatc cactcgccct gctgatctgg ggaatcgttt agaggcgcac ggctgggtgc
     1621 ttgatgcagc gcccggtatg gctgtagacc tacagaccct gtccgagcaa cctcgcccga
     1681 catctccgct cactatcgaa cgggttcacg atacagagat gctgaaaacc tggctgcgcg
     1741 tcatgatgat tggatcggag attccagaag ccggactcaa tattctgctg gacattgtat
     1801 cccaacaggg ctataaagat agtccttccg ctcactacta tcttgggtgg ctcgatggcg
     1861 tgccggtagc aacttcgctg ctctttctgg caggaggcgt tgcagggatt tataatgtgg
     1921 caaccctcct tgcatttcga gggaagggca tagggagcgc attgacggtt gtgcctttat
     1981 tggatgcgcg cgcattgggt tacaggattg gcgtcttgca atcatctccg atgggattca
     2041 atctctacaa acgcctggga ttcaagcagt attgcacatt ttacgcttac ttctggcaag
     2101 ctgcaaacct cgacagcagc gaaaaggaat aactggaacc gtgatataat ccctatattc
     2161 cggcgtgttc cattcatttg taccgatgtc gctaccgtca gtccgtcctg aagagaagga
     2221 gaaagcccgt tgactacctt agacaactca gctacccccg ccaacgcagt accagccagc
     2281 cagagaccgc cccactatct tgacgttcat gcgctgatgc gcccggacaa gccagcgctg
     2341 atctgcgagg atcgcatcct cacctatgcc gaattcaatg cgcgggccag gcgggtcgcc
     2401 aacgttctga gcatgctggg tgtgcaggca ggcgacaggg taggcgtgat tgcgcacaat
     2461 tccatcgagt tgctagaggt tgctggaggg ctgtcgaagt tatctgcgat tggcgtcttg
     2521 ctgagttttc gcctccgcga acaggaaatc gcctacattc tgaacgacag ccaggcgaaa
     2581 gttgctatcg ccggaccagg gctggcaagt gttgtgaacc tggctcgccc ttcagtcgat
     2641 cataagatcg ccttcatcgc catcggagac gacgaaatag gagagtcgga ctggctgctc
     2701 tacgaagcat tacttaccgg ggccagcgcg gaaataccag aaagcgagag cgggttgggt
     2761 tccacgatga gctacacatc aggcacgacg ggtaagccaa agggagctta tcgcccgcgc
     2821 ggcgtatcac ccctgcatat tctgaggctt gtgcagatct tcggattgtc cgagacggac
     2881 gtccacctgc tggctggccc ttactatcac agcgcgccca attttttcac ggcggcgcat
     2941 ctcctgctgg gcgcaacggt cgtgatccag cccaaatacg acccggccga tgccctgcaa
     3001 ctgatcgaac gccatcatat caccacgacc tttatggctc cgacgctgct gcaccggctc
     3061 tgcgacgtgc cacaagacga gttcgcccgc tacgatgtct cttcgctgca ctcgatcata
     3121 ctcggcggcg caccctgtgc ctatgccttg aaggttcgcg ccatagagcg tttcggcgag
     3181 attgtgtggg agttttacgg cgcgaccgag accggctttg tgacgatcct ccggcccgaa
     3241 gatcagttgc gcaagccggg gtcgtgcggc acggtcggtc ctggacagga gattctcctg
     3301 ctcgacgcgg aagggaacga ggtgccggat aatgtccccg gcgaaatgtg ggcgcgcaac
     3361 gacttcctcg ccgagtacta taataagccg gaggcgacgg ccagcaacac aaaggatggc
     3421 ttcttttcgg tcggtgatat cgcctatcgc gatgcggagg gatattattt catttgcgac
     3481 cgcaagatcg acatggtgat ctccggcggc gtcaacatct acccggcgga gatcgaggcc
     3541 gtgctgacag cccatcctgc catcgcggac gttgcagtga tcggcgttcc cgacgaaaag
     3601 tggggcgagt cggtcatggc cgttgtcgaa ctgctgccgg gagtgagcgc caccgcacag
     3661 gagttgatcg agttctgttc cgcgcgcctg gccgattaca aacggcctcg ctcggtcgat
     3721 tttgtcgatc aactcccgcg taacccggcg ggcaagctgc tcaagacccg catccgcgag
     3781 ccatactgga aagacgttgg tcgccggatt taagag
//
LOCUS       DASZAK010001068         1667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_132519, whole genome shotgun sequence.
ACCESSION   DASZAK010001068 DASZAK010000000
VERSION     DASZAK010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132519"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1522
                     /gene="gatA"
                     /locus_tag="VGT44_18890"
     CDS             44..1522
                     /gene="gatA"
                     /locus_tag="VGT44_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV2582933.1"
                     /translation="MSELYQLSIHEAGELLRERKISSVELTKAHLERIREVDGRVKAY
                     TLVTDGLALRQAAEADRRFSNGATVSPLTGIPLAIKDVISTKGITTTCGSNMLKDFKP
                     PYDATVMERLNMAGVVMLGKTNMDEFAMGSSTEHSAFFTTQNPWDLERAPGGSSGGSA
                     AAVAAGMAMGALGSDTGGSIRQPGALCNVVGFKPTYGRVSRYGLVAFASSLDQIGPFA
                     RDARDAALLMQAIAGHDPLDSTSAPEQVPDYSASLTGDIRGLRIGVPAEYWVEGAQPG
                     VVQAVRDDIEKLRELGAIIEEVSLPHTKYGVPVYYIIAPAEASANLARYDGVKYGYSY
                     RDTGDMWEAMEKTRQYGFGQEVKRRIMLGTYSLSSGYYDAYYKRAEKVRALITQDFTR
                     AFERFDVLVSPASPSVAFKIGEITDPYEMYLQDVFTIPANLSGICGVSIPGGFSQGLP
                     VGLQILGNFFGEPTILRVADAFQRVTDYHTRWPQAVEKAKIK"
BASE COUNT          313 a          549 c          525 g          280 t
ORIGIN      
        1 atcattacac gcagtcacta cctggaaagg ggtgtcgtag atcatgagcg aactctatca
       61 attgagcata cacgaggcgg gggaattgct gcgggaacgc aagatctcct cggtggagct
      121 gacgaaagcg catcttgagc gcattcgcga ggtggatggg cgcgtcaaag cctatacgct
      181 ggtgacggat ggcctggcgc tcaggcaggc tgccgaggcc gaccggcgct tcagcaatgg
      241 ggcgaccgtt tcgccgctga cgggcattcc gctggccatc aaggatgtca tcagcacgaa
      301 gggcatcacc accacctgcg gctccaatat gctcaaggac ttcaagccgc cctacgacgc
      361 gaccgtcatg gagcgcctca atatggcggg ggtcgtcatg ctgggcaaga ccaacatgga
      421 cgagttcgcc atgggttctt cgacggagca ttcagccttc ttcaccacgc aaaacccctg
      481 ggacctggaa cgcgcgcctg gcggcagcag cggcggctcg gcggcggcgg tcgcggctgg
      541 catggccatg ggcgcgctcg gcagcgatac gggcggctcg attcgccaac ctggcgcgct
      601 ctgcaacgtc gtcggattca aaccaaccta cgggcgcgtc tcgcgctacg ggctggtggc
      661 ctttgcctcg tcgctcgacc agatcggccc cttcgcgcgc gatgcccgcg acgccgcctt
      721 gctcatgcag gccatcgccg ggcatgaccc gctcgactcg accagcgcgc cggagcaggt
      781 ccccgactac agcgcgtcgc tgacgggcga cattcgcggc ctgcgtatcg gcgtgcctgc
      841 cgaatactgg gtggagggcg cgcagcccgg cgtcgtgcag gccgtgcgcg acgacatcga
      901 gaagcttcgc gagctgggcg cgatcatcga agaggtctcg ctgccgcata cgaaatacgg
      961 cgtgcccgtc tactacatca tcgccccggc ggaagccagc gcgaacctgg cgcgctacga
     1021 cggcgtcaag tacggctatt cctatcgcga caccggtgat atgtgggagg cgatggagaa
     1081 gacgcgccag tacgggttcg gccaggaggt caagcggcgc atcatgctcg gcacctattc
     1141 gctctcgtcc ggctattacg acgcctacta caaacgcgcc gaaaaggtgc gcgcgctcat
     1201 cacgcaggac ttcacgcgcg ccttcgagcg cttcgacgtg ctggtcagcc cggcctcgcc
     1261 ctcggtagcc ttcaagattg gcgagatcac cgatccctac gagatgtact tgcaggacgt
     1321 gttcaccatc ccggccaacc tgtccggcat ctgcggcgtc tccatccccg gcggcttcag
     1381 ccagggcctg cccgtcggcc tgcaaatcct gggcaacttc ttcggcgaac ccaccatcct
     1441 gcgcgtcgcc gacgccttcc agcgcgtcac ggactatcac acccgctggc cgcaggcggt
     1501 ggagaaggcg aagataaagt aggacacatg ggatcccccc aaagggcgac cgcaagggtc
     1561 tccaccccac atccacccca ctcccgccct tacaatgact acgaggccgc ccgacaagct
     1621 gattcgtcat ctttgtaagg gcgggagtgg tagggagtgg ggtggag
//
LOCUS       DASZAK010001069         2840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_99880, whole genome shotgun sequence.
ACCESSION   DASZAK010001069 DASZAK010000000
VERSION     DASZAK010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99880"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1822)
                     /gene="ppdK"
                     /locus_tag="VGT44_18895"
     CDS             complement(<1..1822)
                     /gene="ppdK"
                     /locus_tag="VGT44_18895"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEV2582934.1"
                     /translation="MTQQITRAGLDEAVAQSPNKWVYLFSEGSAKMRDLLGGKGAGVA
                     EMTNAGLPVPPGFTITTEACNAYYDAGKQFPRGMWEQALAALKIVEEQTGKGFGKQGN
                     PLLVSVRSGAKFSMPGMMDTVLNLGINDETVQGLVRLTGDERFAYDAYRRFIQMFSKI
                     VLDTDPRDFEQLLDEYKERAGVKTDAEIPAEALKKLVGDFKQIAERQSGEPFPTDVYQ
                     QLHKAIEAVFSSWNNRRAIDYRNFNKIAHDLGTAVNVQSMVFGNMGSDSGTGVAFTRN
                     PSTGEKQLYGEYLLNAQGEDVVAGIRTPSKISRLHEELPHVFQQFQEIAQRLEKHYRD
                     MQDLEFTIEKGRLFMLQTRSAKRSAPAAVKVAVDMVEEGLITEQEALKRVEPGQVYQL
                     LLPRFDEAEKKRAEQEGRLLAKGLNASPGAASGKAIFDADRAEQLGKAGTPVVLVRPE
                     TSPDDVHGMLVAKGILTARGGATSHAAVVARGLGLPCVAGTEGIRVHEEEHLFRVVGK
                     DIVLREGDDISIDGTTGEVFAGIIRTVDADYDREVDLKKLLTWADKYRVLGVWANADY
                     PRDAKRAVVFGAQGIGLCRTEHMVMEQERLPIVQKMILAET"
     gene            complement(1897..2481)
                     /locus_tag="VGT44_18900"
     CDS             complement(1897..2481)
                     /locus_tag="VGT44_18900"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEV2582935.1"
                     /translation="MSHMFTNRHDPGESQEELERRILEGVSEQERSLRGQDADSPRSG
                     PLHTASYTEEDEEEEGNSSREGDLQDALGLFAEAAQDAATASVELGLGRHFACADYCN
                     QVAEKSAQAVSLLRFGRPVRYDHDLRALGERVNAPPDILNEMAMLTPFHPESFYADTP
                     PEQADEVIDHEAASAYLEYARHVLRWARNIVLNT"
BASE COUNT          533 a          914 c          764 g          629 t
ORIGIN      
        1 tcgtttcggc caggatcatc ttctgtacga taggcaggcg ctcctgctcc ataaccatat
       61 gctcggtgcg gcacaggccg atgccctgtg cgccgaagac gacagcgcgt ttagcgtcgc
      121 gcgggtagtc cgcgttggcc cacacgccca gtacgcgata tttgtcagcc caggtgagca
      181 gcttcttcag atcaacttcg cgatcgtaat cggcgtccac cgtgcggata atgcccgcga
      241 agacctcgcc ggtcgtgcca tcgatggaga tgtcgtcgcc ctcgcgcagc acaatatctt
      301 tgccgacgac gcggaagaga tgctcttcct catggacgcg aatgccttcg gttcctgcca
      361 cgcagggcag gcccaggccg cgcgccacga cagccgcgtg actggtcgcg ccgccgcgcg
      421 ccgtcaggat gcctttcgcc accaacatgc catgcacatc atcggggctg gtctccgggc
      481 gcaccagcac gacaggagtg ccggccttgc ccagttgctc ggcgcggtcc gcgtcgaaga
      541 ttgccttgcc gctggcagcg ccgggcgagg cgttcaaacc tttcgccagc aaacgtccct
      601 cttgttcggc gcgcttcttc tctgcctcgt cgaaacgcgg cagcagcagt tgatagacct
      661 gtcccggctc aacgcgcttg agggcctcct gctcggtaat cagtccctct tccaccatat
      721 cgacggccac cttcaccgcc gccggagcag aacgcttggc cgagcgcgtt tgcagcatga
      781 acaggcgacc cttttcaatc gtgaattcca ggtcctgcat atcgcgataa tgcttctcaa
      841 ggcgctgcgc aatctcctgg aactgctgga agacatgcgg cagctcttcg tgcaggcggc
      901 tgatcttgga gggggtgcga atgccagcca ccacgtcctc gccttgcgca ttgagcaggt
      961 attcgccgta gagctgcttc tcgcccgtgc tggggttgcg cgtgaaggcc acgcccgtgc
     1021 cgctgtcgct gcccatgttg ccaaagacca tgctctgcac attgacagcg gttcccaggt
     1081 cgtgcgcaat cttgttgaag ttgcggtaat cgatagcgcg ccggttattc caggatgaga
     1141 agacggcttc gatggccttg tgcaattgct ggtagacatc ggtcgggaac ggctcgcccg
     1201 actgccgctc ggcaatctgc ttgaaatcgc caaccagctt cttcaacgcc tcggcgggaa
     1261 tttcggcatc ggtcttcacc ccggctcgct ctttatattc atccagcagt tgctcgaagt
     1321 cgcgcgggtc ggtatccagc acaatcttgc tgaacatctg gatgaagcgg cggtaggcgt
     1381 cataggcgaa acgctcgtcg ccggtcagcc tgaccagccc ctgcaccgtc tcgtcgttga
     1441 tgcccaggtt cagcacggtg tccatcatgc ctggcatcga aaatttcgcg ccggagcgca
     1501 ctgaaacgag caggggattg ccctgcttgc cgaagccctt gcccgtctgc tcttcgacga
     1561 tcttcaaggc cgccaacgcc tgctcccaca tacctcgcgg gaactgcttg cccgcgtcgt
     1621 agtaggcatt acacgcctcg gtggtaatcg taaatcccgg tgggacaggc agccccgcgt
     1681 tggtcatctc ggccacgccc gcgcctttgc cgccgagcag gtcgcgcatc ttcgcgctgc
     1741 cttcgctaaa taaatacacc catttgttag gggattgtgc tactgcctca tccaatcccg
     1801 cgcgtgttat ctgctgtgtc attcatagcc tccatttgtt tatttaagca agggtcgccc
     1861 ttccgacccc ctcgacacga aaatgacgca cacccgttac gtattcaata caatatttct
     1921 tgcccaacgc aatacgtggc gcgcatactc tagataggca ctcgccgctt cgtgatcgat
     1981 gacttcgtcg gcctgctccg gcggtgtatc agcataaaat gattcagggt gaaatggcgt
     2041 cagcatggcc atctcgttca ggatatccgg cggcgcgttc acacgctcgc ccagcgcgcg
     2101 caggtcgtgg tcatacctca caggccgtcc aaagcgcagc aggctgaccg cctgggccga
     2161 cttctccgcg acctggttac aataatcagc gcaggcgaaa tgccgaccca aacccagttc
     2221 tacgctggcc gttgccgcat cctgcgcggc ctccgcgaac aaccctaacg cgtcctgcag
     2281 gtctccttct ctgctggaat taccctcctc ttcttcgtcc tcctccgtat acgacgctgt
     2341 atgcaatggc ccggaccggg gggaatcggc gtcctggccg cgtagagaac gctcctgctc
     2401 gctcacgccc tccaggatac gtcgctccag ttcctcctgg ctctcccctg gatcgtggcg
     2461 atttgtaaac atatgagaca tccaacctca tccttgcatg atacctgtac ttttccgcct
     2521 tgtgaacatc atgccttcac ttgccgtact ttatgcagta ctaccgtgct tatattattc
     2581 ctcgtaaagc attgtgtcaa tgttcgcaac acaaagaggt gtaggaacta cttgtcggca
     2641 ttttatcaac cgtaggagcc ggtttaccgt gctcgcagcc gattgatcgg cgcgaatcaa
     2701 agtcgacctg actggctgga ctggacgata atgaactatt acgaacaaga ttggctaacc
     2761 cgtaggcgcc gggtcaccgt gcgcaccgcc gatttatcgg ccaaagagat tgtcaacgcg
     2821 ctccacaggc cgataaatcg
//
LOCUS       DASZAK010001070         1569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_559643, whole genome shotgun sequence.
ACCESSION   DASZAK010001070 DASZAK010000000
VERSION     DASZAK010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559643"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VGT44_18905"
     CDS             <1..225
                     /locus_tag="VGT44_18905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582936.1"
                     /translation="YAAITIFALTRLLPLYSLLAWITFPLAFSNVRAVLAATDRRAFA
                     LGIKRTAMLHLQFGVILAVGIGIAVIAGTR"
     gene            335..907
                     /locus_tag="VGT44_18910"
     CDS             335..907
                     /locus_tag="VGT44_18910"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEV2582937.1"
                     /translation="MVGSSLEQLEEHYDVAITWHSFELHPAGSPPISPQYRERIIAGR
                     PQLLRRAREQYGVEMNPGPLGANSRPALELEKYAETQGKSAAFHVAAMRAYWEQARDI
                     GDQEVLREVAEQVGLNTKDFEQILASEAFDGQVSADVELAHEYGLTGVPALIFADRYL
                     VMGAQPYPMLKQVVERVIEEQKDGSTEEPE"
     gene            913..>1569
                     /locus_tag="VGT44_18915"
     CDS             913..>1569
                     /locus_tag="VGT44_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582938.1"
                     /translation="MFALKLLLSPLLIGVVSLAGRRWGPSVSGWLVGLPLTSGPVVFL
                     LALDQGTAFAARAAQGTLLGLISVVSFCLVYCWCSFRMNWLGSLLIGWCAFFLSTALL
                     LRVSLPLLLAFVCVVGCTALALVLMPRRKEQALPPLAPSWETPLRMVMAATFVLLITG
                     ISGLIGPQLSGLLTPFPMFASILGAFTHRFQGAEAARQSLVGVVIGSFASSVFLAVVA
                     A"
BASE COUNT          261 a          470 c          489 g          349 t
ORIGIN      
        1 tatgccgcca tcaccatctt cgccctgacc cgcctgctgc cgctctacag cctgctggcc
       61 tggatcacct tcccgctcgc attcagcaat gtacgagcgg tgctggctgc gaccgataga
      121 cgagccttcg cgctgggcat caagcggacg gcgatgctgc atttgcagtt tggggtgata
      181 ctggcggtgg ggattgggat agcggtcatt gccggaacaa gataacgggc atcgatgtta
      241 tatactacaa ccagtaccat cacccaatag aaggatttca cctatgtcca ttcgcatcga
      301 tgtctggtcg gactttgttt gacccttttg ctacctggta ggctccagtt tggagcaact
      361 cgaagaacac tacgatgtag ccataacctg gcactcgttt gaattacatc ccgccggttc
      421 gccgcccata tcgccgcaat atcgggaacg catcatcgcg gggcgaccgc agttgctgcg
      481 gcgggcgcgc gagcagtatg gcgtggagat gaaccctggc ccgctcggcg ccaatagcag
      541 acccgcgctg gagcttgaga agtatgccga gacgcagggt aagagcgctg ctttccacgt
      601 cgccgccatg cgcgcttact gggagcaggc cagggatatc ggcgatcagg aggtgctgcg
      661 cgaggtagcg gagcaggtcg gcctcaacac caaagatttc gagcaaatcc tggcgagcga
      721 ggcgttcgat ggacaggtct ccgccgacgt agagctggcg cacgaatacg gcctgaccgg
      781 cgtacccgcg ctgatcttcg ccgaccgcta cctggtgatg ggcgcgcagc cctatccaat
      841 gctgaaacag gtcgtcgagc gagtcataga agaacagaag gacggttcta cggaagagcc
      901 ggaatagcgc ctatgtttgc gctcaagttg ttgttgtcgc cgctgctgat cggtgtagtc
      961 agcctggcgg gccgccgctg ggggccgtcg gtgagcggct ggctcgttgg cctgccactc
     1021 acatcggggc cggtcgtctt cctactcgcg ctggaccagg ggacggcatt cgctgcgcga
     1081 gctgcgcagg gaacgctgct ggggctgatc tcggtcgtga gtttctgcct ggtctactgc
     1141 tggtgttcgt ttcgcatgaa ctggttgggc agcctgttga tcggctggtg cgccttcttt
     1201 ctttcgaccg cactcctgct gcgcgtctcg ctcccgctcc tgctggcctt cgtgtgtgtc
     1261 gtcggatgca ccgcactcgc cctggtattg atgccgcgcc ggaaggagca ggcgctgccg
     1321 ccactcgcgc catcctggga aacgcccctg cgcatggtga tggcggccac cttcgtgctg
     1381 ttgattaccg gcatctccgg cctcatcggt cctcagttga gcggattgct cacgcctttt
     1441 cctatgtttg ccagcatttt aggcgcgttc actcaccgtt ttcaaggagc ggaagctgcc
     1501 cggcagtcgc tcgtcggcgt ggtaatcggc tcgtttgcct cctctgtctt cctggcagtc
     1561 gtggcggcg
//
LOCUS       DASZAK010001071         4299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_346758, whole genome shotgun sequence.
ACCESSION   DASZAK010001071 DASZAK010000000
VERSION     DASZAK010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346758"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="VGT44_18920"
     CDS             complement(<1..600)
                     /locus_tag="VGT44_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2582939.1"
                     /translation="MSADMQTTTSDASTTASHPAGGDAAVVVENLTKRYGANTVVDQL
                     SFTVQRGEIFALLGPNGAGKTTTIETLEGYRRPDVGNVRVLGLDPIREARALKPRIGV
                     MLQQDGLYPGLTAREVVQLFAGYFRSPQHPDTLLDRLGLQHAKKTRYRRLSGGEKRRL
                     ALAVTLVGNPSLLFLDEPTTGMDPQARLATWEIIRELKRG"
     gene            831..1001
                     /locus_tag="VGT44_18925"
     CDS             831..1001
                     /locus_tag="VGT44_18925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEV2582940.1"
                     /translation="MIGNVLWAVIVVLVVLWLIGLLAGIGGGLIHLLLIIALIVLIYN
                     LVVRTRSRRTVL"
     gene            1198..1851
                     /locus_tag="VGT44_18930"
     CDS             1198..1851
                     /locus_tag="VGT44_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582941.1"
                     /translation="MQGETTSTTNATGTAAKSAAPPHHFTGVLFHHEPHQHQPRNINA
                     IHKAEQQEGGLNTRIAIGLTRSVGTMWTAYAFTVLAIIGLLGILGLLNPITILLVGWT
                     SQTLIQLVLLPVIMVGQNVLGRKSELQADEAFKTTMSTYHDIEQIMQHLSAQDAELLR
                     HAKLLIHLLEKNGISLQQLEAEGTTTTHLVDPYAQSPAATNVPAPSAAAGSQEQSAS"
     gene            1994..>4299
                     /locus_tag="VGT44_18935"
     CDS             1994..>4299
                     /locus_tag="VGT44_18935"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV2582942.1"
                     /translation="MSQLERYTAEGQKALMNAREEAASLRHRIIEPEHLALGILKVSD
                     PVIESLFARLQADTARLEETLMFVVGRGNKAILSRPTFGQAARAVLQRAGEIAGRMNT
                     PLVGIEHLLLGVLGDKNGIATGVMENFGITYEAAQEEVESLINSQDQSSLSNYYYRRY
                     SETPTLNLVSRDLSLAALAGKLDPIVGREAELERTMQILMRRSKNNPVLIGQAGVGKT
                     AIAEGLAIRIIEGRVPEHLRHCRVVTLDVGLLTVGTRFRGDFEERLKQIMQEILKAKE
                     LLVVIDELHALVGTGVAEGSIDAANLFKPMLARGEFRCIGATTLDDYRKTIEADPALE
                     RRFQPVMINETTAEETLAVLRGLRDCYADFHRVAIDDDALVAAVQMSSRYVQGRCQPD
                     KALDLVDEAAARIRVQRAGMSDEVHHLRATLQAMQRIKDEAILRGDYTVAAHQRREER
                     QARRSLIDVEDAWLEQRQQERPVVDQQAIAAVVSMWTGIPVEQIAGEEATRLLALEEN
                     LHRRVIGQDEAVQAVAKAIRRARTGIRDSHRPIGSFLFVGPTGVGKTELARALAHTLF
                     GDENALLKLDMSEFMEQHTASRLLGTPPGYVGYDQGGQLIEAVRRRPYSIILFDEIEK
                     AHPGIFDLLLQVLNDGCLTDAQGHTASFQHCVIILTSNAGTAQLRQSQMSFTPARRGK
                     QAQQASALAHLRAQILPALNDVFRPELLNRFDEIVVFHPLEHEHLREIVDIMIAHTRQ
                     RIAEQTITLSVTEPARHLLVEQGYDPTYG"
BASE COUNT          936 a         1247 c         1236 g          880 t
ORIGIN      
        1 gccgcgtttc aactcgcgga tgatctccca ggttgccagg cgcgcctgtg ggtccatgcc
       61 ggtcgtcggc tcatccagga agagcagcga gggattgccg accagcgtta ccgccagcgc
      121 gagccgccgc ttctctccgc cggacagccg ccggtagcgc gtctttttcg catgttgcag
      181 cccgaggcga tccagtagcg tatcaggatg ctgcggcgag cggaagtacc cggcgaacaa
      241 ctgaaccacc tcgcgcgctg ttagtccagg atacaaccca tcctgttgca gcatcacccc
      301 gatgcgtggt ttgagtgcgc gcgcctcgcg gatgggatcg aggcccagta cgcgcacatt
      361 gcccacgtcg gggcggcgat agccttccag cgtttcaatc gtcgtcgtct tgcccgcgcc
      421 atttgggccg agcagcgcga aaatttcacc gcgctgcacg gtgaaactca gttggtcgac
      481 tacggtattc gccccgtagc gtttcgtcaa gttctcaacg acaacggcgg catcccctcc
      541 cgccgggtga ctggcggtgg tcgatgcatc cgaagtcgtt gtctgcatat ccgcagacat
      601 ggtcatctcc ttcaacgtga actatgtgtc actatttcct gtatacctta cagcagtttt
      661 gcgatgatga caagccctta caagccagta atcgaatgca acataaaacg atttcccagg
      721 cgagtacgtg ataagaggta gatgggataa acaatccctg gggcgtttag catgagggaa
      781 atgcctcaat tgctgaaagt gtcacaagtg aagataaagg agcataaaac atgataggaa
      841 atgtcctctg ggctgtaatc gttgtgctgg tcgtgctgtg gctgataggc ttgctggcgg
      901 gtatcggtgg tggccttatc cacctgctgc tcatcattgc gcttatcgta ctcatctaca
      961 acctggttgt acgtacgcgc tcgcgccgca ctgtattata agcaggcgat gcaagaactg
     1021 cggagggatc ggcaaaccga tccctccatt tttttgtctg taaggtctcg tttgagagaa
     1081 agggcaggtt cctacgtata ttcaaataag acacctattc aagaaagtcg aaggatgtta
     1141 tagtgtaggc tatcttcgag taggcaagac attataaaca atttggaggc taataatatg
     1201 caaggagaga ccacatcgac cactaatgca acaggaaccg ccgctaagtc agcggcgccc
     1261 ccgcaccatt ttactggcgt tctatttcat cacgaacccc atcagcacca gccgcgtaat
     1321 atcaatgcta tccacaaagc ggagcagcaa gaaggtggcc tcaataccag gatcgctatt
     1381 ggacttacgc gaagcgtagg aacgatgtgg acggcctatg cctttacggt actggcgata
     1441 atcggactcc tggggatact tggattgctc aatccaatca ctatcttgct tgttgggtgg
     1501 acttctcaaa cactcatcca gcttgtactc ttgccggtga ttatggtggg gcaaaatgtt
     1561 ttaggccgaa agtcggaact ccaggcagac gaggcattta agacaacgat gagtacctac
     1621 cacgatatcg agcaaattat gcagcatctc tcggcgcagg acgccgaact gctgcgccac
     1681 gccaaattgc tcatccattt gctggagaaa aacggcatct cgctgcaaca actggaggct
     1741 gaaggaacca ctactactca cctggtagac ccctacgccc agtccccggc ggccaccaat
     1801 gttcctgctc cttccgccgc tgctggaagc caggagcaga gcgcatcata ggagaccttc
     1861 taaggacttt ccgcttgatt ccacggctcg atgagtccat tcgcctgatt gaactggccg
     1921 gctgacccga tatctcttca tagacaagtt tcaaagcact cgcagaggtg ctatcgcagt
     1981 gaggagtatc agcttgagcc agcttgaacg ttacacggca gagggacaga aggcgttgat
     2041 gaatgcgcga gaagaggcgg catcgcttcg acatcgcatc atcgaacctg aacacctggc
     2101 gctgggtatt ctcaaggtca gcgatccggt gatagagtcg ctctttgctc gactccaggc
     2161 cgatacggcc cgcctggaag agaccctgat gtttgtcgtg ggacgaggga ataaggcgat
     2221 cttgagcagg ccgaccttcg gacaggcggc acgagccgtc ttgcagcgcg ccggggagat
     2281 cgccgggcgc atgaacacgc cgctggtggg catcgaacat ctgctgctcg gcgtgctggg
     2341 tgataaaaac ggcatcgcga cgggtgtgat ggagaacttt ggcatcacct atgaggctgc
     2401 gcaggaggaa gtcgaatcgc tgatcaacag ccaggatcaa tcatcactgt caaattacta
     2461 ttaccggcgc tatagcgaaa cgccgacgct caacctggtg agccgcgacc tttctctggc
     2521 agccctggcc ggaaaactcg accccatcgt tgggcgcgaa gctgaacttg agcgtaccat
     2581 gcaaatcttg atgcgccgct cgaagaataa tccggtcttg atcggccagg ctggcgtcgg
     2641 caaaacggcc attgcggagg gcctggccat acgcatcatc gaggggcgtg tccccgaaca
     2701 cctgcgacac tgccgcgttg tgacgctgga tgtcggcctg ctgacggtcg gcacccgctt
     2761 ccgtggcgac tttgaagaac gtttgaagca gatcatgcag gagatcctca aggccaaaga
     2821 actgctggtt gtgatcgatg aactccacgc gctcgtcggc acaggtgttg cggagggttc
     2881 tatcgatgcc gccaacctct tcaagccgat gctggcgcgc ggtgaatttc gctgtatcgg
     2941 ggcgaccacc ctggatgatt accgcaagac gatcgaggcc gatccggccc tggagcgtcg
     3001 cttccagccc gtgatgatca atgagacgac ggcggaagaa acgttggccg tgctgcgcgg
     3061 cttgcgtgat tgctatgccg attttcaccg cgttgcgata gatgacgacg cgctggtcgc
     3121 tgccgtccag atgtcttcgc gctacgtgca gggtcgctgc cagccggata aggcgctcga
     3181 tctggtcgac gaggcggcgg cgcgcatccg cgtacaacgt gcgggtatgt ccgacgaagt
     3241 gcatcacctg cgcgcgacac tgcaagccat gcagcgtatc aaggacgagg ccatcttgcg
     3301 cggcgattat accgtcgcgg cgcatcagcg gcgcgaggag cggcaggcgc gtcgctcgct
     3361 gatcgatgtc gaggacgcct ggcttgaaca gcgccagcag gaacgcccgg tcgtcgatca
     3421 gcaggccatc gccgctgttg tctcgatgtg gaccggcatc cccgtagagc agattgcagg
     3481 tgaagaagcc acgcgcctgc tcgcgctcga agagaacctg caccggcggg tcattggcca
     3541 ggatgaagcg gtgcaggcag tcgcgaaagc cattcgccgc gcgcgcaccg gcattcgcga
     3601 tagccatcga ccaataggct ccttcctgtt cgttggccca acgggcgtgg gcaagaccga
     3661 gctggctcgc gcgctggctc acacgctttt cggcgatgaa aacgcgctgc tcaagctgga
     3721 tatgtccgaa tttatggaac aacataccgc ttctcgtctg ctcggtacgc ctcccggcta
     3781 tgtcggctac gaccagggcg gccagctcat cgaggcggtg cggcggcgcc cctacagcat
     3841 catcctgttc gacgagatag agaaagcgca ccccggcatt ttcgacctgc tgctccaggt
     3901 gctgaatgac ggctgcctga ccgatgcgca gggccacacg gccagtttcc agcattgcgt
     3961 catcatcctg acctcgaatg ctggtacagc gcagcttcgc cagagccaga tgtcctttac
     4021 accagcgcgc cgcggtaagc aagcgcagca ggccagtgcg cttgcgcacc tgcgcgccca
     4081 aattctgcct gccctcaacg acgtgttccg cccagaactg ctcaatcgtt tcgatgagat
     4141 cgtcgtcttt catcccctgg aacacgaaca cctgcgcgag attgtcgata tcatgatcgc
     4201 gcacacccgg cagcgaatcg ccgaacaaac gatcacgctc agcgtcaccg aacccgcgcg
     4261 ccacttgctc gtggaacagg gctacgatcc gacctacgg
//
LOCUS       DASZAK010001072         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_277184, whole genome shotgun sequence.
ACCESSION   DASZAK010001072 DASZAK010000000
VERSION     DASZAK010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277184"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..434
                     /locus_tag="VGT44_18940"
     CDS             84..434
                     /locus_tag="VGT44_18940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEV2582943.1"
                     /translation="MPQANVVMNVRQVNATASIIDIQGEVSAFAEKVLMEAYTEASSP
                     TTRTIILNFSGLEYMNSSGIGLIVTLLIRVNRQKQHLLAFGLSEHYRRIFEITRLNDA
                     IRVYHSEDEALAAA"
     gene            450..2129
                     /locus_tag="VGT44_18945"
     CDS             450..2129
                     /locus_tag="VGT44_18945"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEV2582944.1"
                     /translation="MPVYQVSSRSVPEVPPPVKKPRRLPPQWSIAIHFIIAVGGAGGI
                     FALLTIFKGPTDTIFLLLPVLVYLAAMQFQSYNPNIPAFFFTVFFRRTPAYLLFTVCF
                     AFVYYGAITGIELFIHQATVSHIILITTALTWTIMLDPVRVFVQRRIERRFNPLDREV
                     VKEIEAFTETLREEIDLDQLRERFLTVIQHALQPYFVSLWVQAFNNHRKQAGSTEVIV
                     VADDDPLLAYLLKHPGVLEIDRLRMDSPAIQGLKLHAAELLVPLTSQGELIGLVVLGS
                     HLKGEVYTNEELKILVTLAPQVAPSLRVAQLVQEQQAQVLERERIEQELRTAQAIQHA
                     FLPKEVPVFPGWQLVTYYRPAREVGGDFYDFLPLKGGRWGLIIGDVTGKGVPAALVMA
                     TVHTMLRSTAQGSLSPSEVLARVNDLLVPDIPQGMFVTCFFALLDPASGRLYYSNAGH
                     EPPYRQQDRNVTELWATGMPLGMLPGSRYEEHEATITPGESLIFYSDGLVEAHSPAKE
                     MFGFPRMQKVLEERCEDVSLIETLLAELHSFTGEGWEQEDDVTLVSLQRTP"
     gene            2134..2907
                     /locus_tag="VGT44_18950"
     CDS             2134..2907
                     /locus_tag="VGT44_18950"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2582945.1"
                     /translation="MLKQSIRCLLFDLGGTLWKHGEEGVTLVYERAANLYALAHLRRI
                     AGNNVFAGVSEMEAGAQLRKVVERAIRVKTRENPLYEPDFAQAATEALQTLGIPNVTT
                     SLGGMIYEALRVRSVDSRLLFEDAIQTLATLKARGYTLGVVTNRHYGGEPFHQDLCEM
                     GLLDYFDYNHMAISADLGIRKPQPGIFTHALNGLCVTPAETAMVGDSLKADIAGAKAL
                     NITAIWKPYRLHRNRRDAEEDGIVPDRTIVQLKDLLDLF"
     gene            2988..3467
                     /locus_tag="VGT44_18955"
     CDS             2988..3467
                     /locus_tag="VGT44_18955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEV2582946.1"
                     /translation="MSNTLGASVQKVQDVLKSLGFTGEVVVFPDSTRTAAEAAQAVGC
                     DVGQIVKSLVFRGKHTGKPVLVEASGANRVNERHVGEMLGEPIEKADADFVRQHTGFA
                     VGGVPPVGHAGPIATFIDEDLLQYQEIWAAAGNPHAVFRLTPGELQALTGGKVITIT"
BASE COUNT          797 a          933 c         1010 g          782 t
ORIGIN      
        1 ctcggacggg cgaccatgaa ggctcgcccc tactggccaa atcgacatcg tcgctagaat
       61 gcatagtatc aaggagaaga ttcatgcccc aggccaatgt tgtaatgaac gtccgccagg
      121 ttaacgccac tgccagcatt atcgacatcc agggagaggt ttcagccttt gctgaaaagg
      181 tgctgatgga ggcgtacacc gaggccagct cgccgacgac gcgaacgatt attctgaact
      241 tcagcggatt ggaatacatg aacagtagtg gtatcgggct gatcgttacg ctacttatcc
      301 gcgtcaatcg ccagaaacag cacttgctgg cattcggttt aagcgaacac taccggcgta
      361 tctttgagat cacgcgcctg aacgacgcca ttcgagtgta tcattccgaa gacgaagctc
      421 tggcagcggc atagagaagg actagcgcta tgcctgttta ccaggtctca tcgagaagtg
      481 tgccggaggt ccccccaccc gtaaagaagc cccggcgctt gcctccgcaa tggtcgattg
      541 ctatccactt tattattgca gtaggcggcg cgggcggcat ttttgccttg ttgacgatat
      601 tcaaaggacc aaccgatacc atcttcctcc tcctgcccgt cttagtgtac ctggctgcta
      661 tgcagtttca gagctacaac cccaacattc cggcgttctt ttttactgtg tttttccgca
      721 gaacaccggc ctatctcttg tttacggtct gcttcgcctt cgtttactat ggcgcgatca
      781 ccgggattga attattcatc caccaggcca ccgtttcgca tatcatcctt atcacgacgg
      841 cgctcacctg gacgatcatg cttgatcccg tgcgtgtgtt tgtgcagagg cgcattgaac
      901 gccgctttaa tccacttgat cgagaggtgg tcaaagaaat cgaagccttt accgagacgc
      961 tgcgcgagga gatcgatctt gaccagttgc gcgagcgctt tctcacggtc atccagcacg
     1021 ccttgcagcc gtattttgtc tccttatggg tgcaggcatt caacaaccat cgaaaacagg
     1081 ctggatcgac cgaggtaatc gtagttgccg acgatgatcc actgctggcc tatttattga
     1141 agcatcccgg cgttctagaa atagatcgcc ttcgaatgga ttcgccagcc atacaagggc
     1201 tgaaactcca cgccgcggaa ctgcttgttc ccttgaccag ccagggtgaa ctgattggcc
     1261 tggtcgtact ggggtcgcat ctcaagggtg aagtctatac caacgaggaa ctcaagatcc
     1321 ttgtgacgct cgctcctcag gtagccccgt cgctgcgtgt ggcgcaactg gtgcaagaac
     1381 aacaggcgca ggtactcgaa cgcgaacgca ttgagcaaga gttacgcacc gcgcaagcaa
     1441 ttcaacacgc tttcttgccc aaagaggtgc cagtcttccc cggctggcag ctcgtcactt
     1501 actaccggcc cgcgcgcgag gttgggggcg acttttatga ctttcttccc ttgaaaggtg
     1561 gccggtgggg tcttatcatt ggagatgtga ccggtaaagg cgtcccggca gccctggtga
     1621 tggcgacggt tcatacaatg ctgcggagta ccgcgcaagg atcgctctcg ccgagcgagg
     1681 tgttggcacg ggtcaacgac ctgctcgtgc ccgatattcc gcaagggatg tttgtgacgt
     1741 gcttctttgc tctgcttgac ccggctagcg ggcggttata ctactccaac gccgggcatg
     1801 aaccgcccta tcgccagcaa gatcggaacg tgacggaact gtgggcgacg ggtatgcctc
     1861 tggggatgct gcctggctcg cgctacgagg agcatgaagc cactattacc cctggagaga
     1921 gcctcatctt ttacagtgat ggcctggtgg aagcgcatag tcctgcaaaa gagatgttcg
     1981 gctttccgcg tatgcagaag gtactcgaag agcgatgcga agacgtatcg ctgatcgaga
     2041 cattgctcgc agaactccac agctttactg gtgagggatg ggagcaggag gatgatgtga
     2101 cattggtatc gttgcaaagg acgccataaa aaaatgctta aacaatctat tcgctgcctg
     2161 ttgttcgatc tcggtggcac actctggaag cacggagaag agggtgtgac cctggtctac
     2221 gaacgggcgg ccaatttata cgccctcgcg cacctgcgtc gcatagctgg taacaatgtg
     2281 tttgctggcg taagtgaaat ggaggcaggc gcacaattac gcaaagtggt tgagagggcc
     2341 atccgagtca agacgcgcga aaaccccctc tacgaaccgg attttgccca ggcggccacc
     2401 gaggcgctgc aaactctggg tataccaaac gtgacaacca gcctgggagg catgatctac
     2461 gaggcgctgc gcgttcgttc agtggattcg cgccttttgt ttgaagatgc tattcaaaca
     2521 ttggcgaccc tcaaagcgcg cgggtatacg cttggggtcg tgaccaatcg ccactatgga
     2581 ggcgaaccat tccatcaaga cttgtgcgaa atgggcctgc tggactactt cgattacaat
     2641 cacatggcca tttccgccga tttaggcatc cgcaaaccgc aacccggcat cttcacacat
     2701 gcgctgaatg ggctatgtgt cactcccgcg gaaaccgcca tggtcggcga ttcgttgaaa
     2761 gcggatatcg ccggtgcgaa agcgctgaac atcacggcga tctggaaacc ttatcggttg
     2821 cacagaaaca ggcgcgacgc agaagaagat ggcattgtgc cggataggac aattgtgcaa
     2881 ttgaaagatc tgctcgatct attttagtca gaggggtagt aagctgatcg taggcgcacg
     2941 gtaaaccggc gcctacgatg agtcaattgc tagaaagagg attactaatg tcgaatacac
     3001 tcggcgcgag tgtgcagaag gtgcaggatg tactgaaatc actcggcttt acgggtgagg
     3061 ttgttgtctt tcccgactcg acgcgcacag cagcggaggc ggctcaagct gtgggttgcg
     3121 atgtggggca gattgtgaag tcgctggtat tcaggggcaa gcacacaggc aagccggtgc
     3181 ttgtcgaggc gagtggggcg aatcgcgtga atgagcggca cgtcggcgaa atgttgggcg
     3241 agccgattga gaaggccgac gctgattttg tgcgccagca taccggcttc gcggtcggtg
     3301 gcgtgccgcc agtcggccac gcgggaccaa tcgcaacctt catcgacgag gatttgttgc
     3361 aataccagga gatctgggca gcggcaggca atccccacgc ggtattccgg ttgacgccgg
     3421 gcgagttgca agcgctaacc ggggggaaag tgatcactat tacatagaca gagtcctgtc
     3481 atgctgagcg cagcgaagca tctctcggcc catctagaca ag
//
LOCUS       DASZAK010001073         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_169533, whole genome shotgun sequence.
ACCESSION   DASZAK010001073 DASZAK010000000
VERSION     DASZAK010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169533"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VGT44_18960"
     CDS             complement(<1..923)
                     /locus_tag="VGT44_18960"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEV2582947.1"
                     /translation="MTMIETSPDEQSIFATPETVYREQEPAREVGTPGGPMDEKITYR
                     QQVSFCGKPRCRKCRDGIGHGPYWFAYSQTPEGRTVRTYIGKQLPPGISAPVSSTASD
                     TDIAAQPMTPEAARDGSEIEALNRLLAQNSSDEATALRLMIALAKAKRRGEALRIYKR
                     YTSLLQSHTGRTPSLEMQDLYETIQRGEDISTSLPEPDASNPDAPHPDAHKGHPYIST
                     DPTSQAGKDNTRLVYGTSIQGRSNQSPLVGRDGELERLRRLLSTAQHGMAARSAESQG
                     SLRSPALALQRVQPQYVVLMGESGIGKTRLA"
     gene            complement(995..>2287)
                     /locus_tag="VGT44_18965"
     CDS             complement(995..>2287)
                     /locus_tag="VGT44_18965"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2582948.1"
                     /translation="HRDIKPENILIHQEDRRLVITDFGIARSLEPGARVGKTITVRGT
                     VGYMAPEQTSGVVDPRSDQFGSAVVLYEMLTGYHPFDSTGGGPIIPPTSLNHELPSGL
                     DTVIARGLAPRPEQRYTDMMEFLREFNTALRPSGSMRAITPSAPSAPQRDSQRIPAAR
                     SVPNPITPANLSGKERGDSTGRQQAVRINTSSVREKCQEGDALLKQQQYTQALQAYEE
                     ALRLDARNFHAWNGKGTAHYSLGNYKKAYEAYQRATEIDADNPVVWVSTGLALNRLAR
                     HSQALVHFERALALDPSYVTAWNGKADTLLTMNMPEDAMTAYMKALAYDQQSFSAWNG
                     LGNARTYLRDYPGAVEAYDHALLVNPRSVVAWCNKAEALMRYGHTFAAIDALNEATEL
                     DRSYAPAWTLKAEAYEAVGNHQEAQKARIRARPWGTMG"
BASE COUNT          373 a          668 c          739 g          507 t
ORIGIN      
        1 tccgccaggc gcgtcttgcc gatgcccgat tcgcccatca agaccacgta ctgcggctgc
       61 acccgttgca atgccagagc cggtgatcgg agcgacccct gagactcggc agacctggct
      121 gccatcccat gttgcgccgt cgacagcaag cgccgcagtc tctctagctc tccgtcgcgt
      181 ccaaccagcg gactctgatt gctgcgcccc tgtatactgg tcccatacac caatctcgta
      241 ttatctttgc cagcctggga tgtggggtct gtcgatatgt agggatgccc cttgtgggca
      301 tccgggtgtg gtgcatccgg gtttgaggcg tctggctctg gtagcgacgt tgaaatgtcc
      361 tcgccgcgtt gtatcgtttc gtacaaatcc tgcatttcga gggatggcgt acgaccggtg
      421 tgggactgca agagagaggt gtaacgcttg taaatgcgta gtgcctcgcc gcgccgtttg
      481 gccttcgcca gcgcgatcat caggcgcagt gcggtcgcct cgtcagacga attctgcgcc
      541 agcaggcggt ttaacgcctc gatttcgctc ccgtcgcggg cggcttcggg cgtcatgggt
      601 tgtgctgcaa tgtcggtatc agaagcagtc gaggatacag gagcagatat gccaggtgga
      661 agctgtttgc cgatgtaagt gcgcaccgtg cggccttcgg gggtctggct gtaagcaaac
      721 cagtacggac catgtccgat gccatcgcgg cacttgcggc atcgcggctt cccgcaaaag
      781 ctcacctgct ggcgataggt gatcttttcg tccatcggcc ctcctggtgt gccaacctcc
      841 cttgccggct cttgctccct atacaccgtt tcaggagtgg cgaaaatgct ctgctcatca
      901 ggggatgtct caatcattgt cattataacg caatcgtcaa catttttcag gctcaactaa
      961 tgatctgaaa ttatggacct catagcagca gttattaccc catcgtaccc cagggcctgg
     1021 cgcgaatgcg cgccttctgc gcctcctggt ggttgccgac cgcttcgtat gcctcggcct
     1081 tgagcgtcca ggcgggggcg tagctgcggt ctagctcggt ggcctcgttg agggcgtcga
     1141 tggcggcgaa ggtgtggccg tagcgcatga gggcttcggc tttgttgcac caggccacga
     1201 cgctgcgcgg gttgacaagc agagcatggt cgtaagcttc cactgcgccg ggatagtcgc
     1261 gcaggtaggt gcgggcgttg cccaggccgt tccaggccga gaagctttgc tggtcgtagg
     1321 ccagcgcctt catgtaggcg gtcatggcgt cttccggcat gttcatggtc agcagtgtat
     1381 cggccttgcc gttccaggcc gtcacatacg agggatcgag cgcgagggcg cgttcaaaat
     1441 gtaccagcgc ctgcgagtgg cgcgccaggc ggttgagcgc caggccggtg ctgacccaca
     1501 ccacagggtt gtcggcgtcg atctcggtgg cacgttggta agcttcatag gcttttttgt
     1561 agttgcccag gctatagtgt gccgtgcctt tgccgttcca ggcgtggaaa ttgcgcgcgt
     1621 ccaggcgtaa cgcctcttca taggcttgca gcgcctgcgt gtactgttgc tgcttgagca
     1681 aggcgtcgcc ctcctggcac ttctcgcgta cactggaagt gttgatgcgc actgcctgct
     1741 ggcggcccgt ggagtcgccg cgctcttttc ccgataggtt cgcgggcgta atcgggttgg
     1801 gaacgctccg cgcggctgga atgcgctgcg agtcgcgctg aggagccgag ggagcggaag
     1861 gagtgatggc gcgcatactc ccacttgggc gcagggcagt gttgaactcg cgcaggaact
     1921 ccatcatgtc ggtgtagcgc tgctccggcc tgggagcaag cccgcgcgca atcactgtgt
     1981 ccaacccgct cggcagttcg tgattcaggc tggtcggcgg gatgatcggc ccgccgccgg
     2041 tcgagtcgaa ggggtggtag ccggtcagca tctcatagag gacaaccgcg ctgccaaact
     2101 ggtcgctgcg cggatcgaca acgccgctgg tctgctccgg ggccatatag cccacggtgc
     2161 cgcgtacggt gatggtcttg cccacgcgcg cacccggttc gagcgagcgc gcgatgccga
     2221 aatcggtgat aaccaggcga cggtcttcct ggtgaatcag gatgttttcg ggcttgatat
     2281 cgcggtg
//
LOCUS       DASZAK010001074         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_667956, whole genome shotgun sequence.
ACCESSION   DASZAK010001074 DASZAK010000000
VERSION     DASZAK010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667956"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..546
                     /gene="rpsU"
                     /locus_tag="VGT44_18970"
     CDS             355..546
                     /gene="rpsU"
                     /locus_tag="VGT44_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002808376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV2582949.1"
                     /translation="MSEVKVNPGDSFEQALKRFNRKVQQDGVLSEARRRTHYESPQAR
                     RKRKAAAQRRKQRRSRPVS"
     gene            complement(643..1407)
                     /locus_tag="VGT44_18975"
     CDS             complement(643..1407)
                     /locus_tag="VGT44_18975"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2582950.1"
                     /translation="MRIGINMAWHNWQDILRMARAADEYGFDSIGFLDHYHSHILEQD
                     YLCGWSMYGALAMATSRIHFVPMVIDRMNYLPGVLAKETSTLSIISDGRFELGIGAGD
                     YFEEARAWGLPVPPAAERIAGLRETVEALRQVWRGENTTFEGAYIHLKDAACVPAPPA
                     PPRVVVGIGNSKRLLSDAVQYADELNVYSDDDFIALAQQAIEASGRNVTLSTLVWGLR
                     DDLADKLPAWETMGVTRTFVSLWKLEEQLAQLARLL"
     gene            complement(1574..2584)
                     /locus_tag="VGT44_18980"
     CDS             complement(1574..2584)
                     /locus_tag="VGT44_18980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEV2582951.1"
                     /translation="MHIDAKPIDLNLTTPFRISRAVSQTAPNVIVQINHKDWTGYGEA
                     APDEFYGETQETVLACVAAFAGNLGDDPFAIEDTMSRLDHIIRLNPSAKAAVDMALYD
                     LVGKMLNAPLYRLLGLNPANTPQTSFTLGIDTPANMAKKALLASQYPILKVKVGTKHD
                     LDNMKAIREVSAATIRVDANTAWTPKEAIKMINALADYNIEFVEQPITPRDLDGLRLI
                     RENVPLPIIADESCITVDDIPRIAGCVDGINIKLMKCGGVRHALKMIHVARAHNLRIM
                     IGCMIESSLAITAAAHLTPLVDYADLDGHLLIDNDPFEGVKVVNGKLMLPDTPGLGVR
                     KT"
BASE COUNT          596 a          715 c          860 g          680 t
ORIGIN      
        1 ttaatcagtg atgtcagcgt tgacatatgc tcactccttt gtttcgatct aactggcgct
       61 tgaaacactt ccaagcgcgt ctgcaatttg gctttcatgc atctatgttt ccctaacggg
      121 acaatatacg ggacgataaa gaagttataa atacatcgta acacgatata attataccag
      181 ataaagattg gatcgtcaag tatgaatggg agaacagttt cggttcttca taggaatttt
      241 gtcggaaagg gtagtgcatt ttctgtccag gtatgctata ctctgtggac gttgaagagc
      301 gtactaatcc attgagaggt tcggcagaac aataaactgc gttggtgatg attcatgagt
      361 gaagtaaaag tcaatcctgg cgattcgttt gagcaggcgc tgaagcgctt taaccgcaaa
      421 gtccagcaag atggcgttct ttcggaagcg cgtcgccgca cgcattacga aagcccgcag
      481 gcgcgccgca aacgcaaggc cgccgcgcag cgtcgcaagc agcgccgctc tcgcccggtg
      541 agctaggctc tcggttgctg gcgagaaaat aagagggtat tacgcaatgt aacccagaat
      601 gaccgttgtg gtcacgctgg gttatttgtg ttgtccgaat gatcacagca gtcgcgccag
      661 ttgtgcgagt tgttcttcga gtttccagag cgatacgaag gtgcgggtga cgcccattgt
      721 ttcccaggcg ggcagcttgt cggcgaggtc gtcgcgcaga ccccatacga gcgtggagag
      781 ggttacgttg cgcccggatg cttcgatggc ttgctgtgcc agggcgatga agtcgtcgtc
      841 gctgtagacg ttcagttcgt cggcgtactg gacggcatcg ctgagcaggc gtttggagtt
      901 gccgatgccg actaccacgc gcggcggcgc tgggggagca ggcacacagg cggcatcttt
      961 gaggtggata taggcaccct cgaaggttgt gttctcgccg cgccaaacct ggcgaagcgc
     1021 ctcgaccgtt tcgcgcagcc cggctatgcg ctcggcggct ggcggaacgg gcaggcccca
     1081 ggctcgcgcc tcctcgaagt agtcgcccgc gccgatgccc agctcgaaac gcccatcgct
     1141 gatgatcgag agcgtggatg tttccttcgc cagcacaccg gggaggtagt tcatgcgatc
     1201 aatgaccatc ggcacgaagt ggatgcgcga ggttgccata gccagcgcac catacattga
     1261 ccagccgcag agataatcct gctccaaaat gtgggaatga tagtggtcga gaaagccgat
     1321 ggagtcgaag ccgtattcgt ccgcagctcg cgccatcctg agaatatcct gccagttgtg
     1381 ccatgccata ttgatgccga tgcgcattgt cttcttctct cctttagaat ctgtctgaac
     1441 ggaacgtgtt tcgcaggaga tagtatagcc gacaatactt gacagagcat gtcaggaggt
     1501 aatggcaagc gagctttcga tatagaagag tttccgggat cacatcgttt ggatagctgg
     1561 tagacggtga gcttcaggtt ttccgtactc ccaacccagg cgtatccggt aacatcaact
     1621 tgccgttgac cactttgaca ccttcaaacg ggtcattgtc gatgagtaaa tgtccgtcaa
     1681 gatcagcgta gtcaaccagg ggcgtcagat gtgcagcggc agtaattgcc agcgagcttt
     1741 cgatcataca gccgatcatg atgcgcaggt tgtgcgctct tgcgacgtgg atcatcttca
     1801 aggcgtggcg cactcctccg catttcatca gcttgatgtt gatgccatcc acacacccgg
     1861 cgatgcgcgg gatatcatcg acggttatgc agctttcgtc ggcgataatc ggcaggggaa
     1921 cgttctcgcg gataaggcgc aggccgtcca ggtcgcgcgg cgtgatgggc tgctcgacga
     1981 actcgatgtt gtagtcggcc agcgcgttaa tcatcttgat ggcctctttg ggcgtccagg
     2041 cggtattggc atcgacgcgg atggtggccg cgctgacttc gcgaatggcc ttcatgttgt
     2101 ccaggtcgtg ttttgtgcct actttgactt tgaggatggg gtactggctg gccagcaggg
     2161 ccttttttgc catgttggcg ggcgtatcga tacccagggt gaacgaggtt tgcggtgtat
     2221 tggccgggtt cagtcccagc agcctgtaga ggggcgcgtt cagcatcttg ccgaccaggt
     2281 cgtacaaggc catgtctacc gcggccttcg cggatggatt gaggcggatg atatgatcca
     2341 ggcggctcat tgtatcttcg atggcaaagg gatcatcgcc caggttgccc gcgaaggccg
     2401 cgacgcaggc cagcaccgtc tcctgcgttt cgccgtaaaa ctcgtccggc gcggcctcac
     2461 cgtagcctgt ccagtccttg tggttgatct ggacaatcac gtttggggcg gtctgcgaga
     2521 cagcgcgcga gatgcgaaac ggcgtggtga gattgaggtc gataggtttg gcgtcaatgt
     2581 gcatggcgtc ctcttccttg tggcttcatg atagttgtct tgttagtatt atgcaacaag
     2641 ttcatcatcc ccgtgatata aatacgtaac gaatacattg gttgctttga ctattttcta
     2701 aaaggagatg aaaagatatg gctgcaacga cccattacga tgcgattgtg attggcgcgg
     2761 ggcaggcggg cggaccgctt tcgactgctt tggcgcaggc aggacggagg accgctatca
     2821 tcgagcgggt gcatgtgggc ggcacctgta t
//
LOCUS       DASZAK010001075         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_346804, whole genome shotgun sequence.
ACCESSION   DASZAK010001075 DASZAK010000000
VERSION     DASZAK010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346804"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="VGT44_18985"
     CDS             <1..1326
                     /locus_tag="VGT44_18985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEV2582952.1"
                     /translation="AEQQRMNEKEVHFRGQLQEAYDQQQVLMQELDRLYREQALAAVT
                     DAVTGLPNHRAVMGRLDEEVSRSLRYETSFAVIFVDLDHFKRVNDTWGHRAGDAILRE
                     VASRLSTNVRLEDFVGRYGGEEFAVILTGTDVVGASQTAERLLVALNSQPCFWETDDA
                     QEVASIPISGSFGLALYQLHGTTREELIESADYAMYQAKRGGRNRVCIADVDRLAQEQ
                     PLVRADEREEDSVSSSSRVELVQLTTGLHALTAAATARDGYTAGHAHRMVELAVATAR
                     QLGQPEEDLNMLRLSAVLHDIGKIGIPDAILHKPGKLTDDEWLIMRTHPDIGYHILQE
                     VGGVFQQLANVVVAHHERWDGRGYPRHLAGEDIPLHSRILTVVDSFDAMTSRRVYREP
                     MTVKQARAELLRCSGTQFDPNIVEAFLSALDNAEGLLQTGTQVVPALPA"
     gene            complement(1355..2350)
                     /locus_tag="VGT44_18990"
     CDS             complement(1355..2350)
                     /locus_tag="VGT44_18990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2582953.1"
                     /translation="MVAIAKKALKHGEYRRVKPSAYYAEDEIVHEVDFREDEEEERDE
                     EDESGEDEEQDNHTTDNAIRQYLAEIGRFPLLTADQELSLAWRVTEGDMEAQQRLVEA
                     NLRLVVSIAKRYNTHGISLLDLVQEGNLGLIRAAQKFDPARGFRFSTYATWWIRQAIS
                     RAVAEYTRTIHVPVHVVEVIYKIRRITRALYQELGRDPLAEEIAKVAHISKERVVELQ
                     SMAETPISLDAPLADDEQYHLADTLEDTSASTPADVVAHQALRAQIGLALDILNLRER
                     QVIELRYGLHDGYCHTLEELSSYFKLTRERIRQIEVKALRTLRHPIQAHLLRDFA"
BASE COUNT          566 a          852 c          798 g          595 t
ORIGIN      
        1 gccgaacagc agcgcatgaa tgagaaggaa gtacattttc gcgggcaact acaagaagca
       61 tacgaccagc agcaggtgtt gatgcaggaa ctggaccgcc tctaccgcga acaggcgctg
      121 gcggcggtga ccgacgccgt caccggcctg cccaatcatc gcgccgtgat gggtcgcctg
      181 gatgaagagg tgtcgcgcag cctgcgttac gagacctcgt tcgctgtcat cttcgttgac
      241 ctcgaccact tcaaacgggt caacgacacg tggggacacc gcgccggtga cgctatcctg
      301 cgcgaagtcg cgtcacgcct gagtaccaac gtgcgcctgg aagatttcgt cgggcgctat
      361 ggcggtgaag aattcgcggt gatcttgacc ggcacagacg tggtgggcgc gagtcaaacg
      421 gcggagcgat tgctggtcgc cctcaactcc cagccatgtt tctgggagac agacgatgcg
      481 caagaggtgg cttccatccc gatcagcggc agtttcggcc tggcgctgta tcaactgcac
      541 ggcacgaccc gcgaagaatt gatcgagagc gccgattacg ccatgtacca ggcgaaacgc
      601 ggcggcagga atcgcgtctg tatcgccgac gttgatcgtc ttgctcaaga gcaaccactc
      661 gttcgcgcag atgagcgaga agaggatagc gtttcttcaa gcagccgcgt cgaactggtg
      721 cagctgacta ccggcctaca cgcgctcaca gcggcggcaa cggcgcgcga cggctataca
      781 gcaggacacg cgcatcgcat ggtcgagctg gcggttgcta ccgcccggca gcttggccag
      841 cccgaagagg acttgaacat gctgcgtttg agcgccgtcc tgcacgatat cggcaaaatt
      901 ggtattcccg acgccattct gcacaaaccc ggcaaactga ccgacgatga atggctcatc
      961 atgcgtacgc accccgatat cgggtatcac atcctccagg aggtcggcgg cgtcttccag
     1021 cagctcgcca acgtggttgt cgcccatcac gaacgctggg acggcagagg ctatccccgc
     1081 caccttgcag gcgaagacat cccgctccac tcgcgcatcc tgacggtggt agactccttc
     1141 gacgccatga cctcgcgccg cgtctaccgc gagccaatga cggtcaagca ggccagagcc
     1201 gaactgctgc gctgctccgg cacccagttc gatcccaaca tcgtcgaagc cttcctctcc
     1261 gcgttggaca acgcggaagg attgctgcaa acaggtacgc aagtcgtgcc tgctctaccc
     1321 gcataaacga agctggaaaa tatcctgctg cgcgctacgc gaaatcgcgc agcaggtgcg
     1381 cctggatggg gtggcgcagg gtgcgcagcg ccttgacctc gatctggcga atacgctcgc
     1441 gagtcagctt gaagtaggag ctgagttctt cgagcgtatg gcagtagcca tcgtggagac
     1501 catagcgcag ttcgatgacc tggcgctcgc gcaggttgag gatgtcgagc gccaggccga
     1561 tctgggcgcg cagcgcctgg tgcgccacga catcggctgg ggtgctggcg ctggtgtctt
     1621 ccagcgtatc ggcgagatga tattgctcgt cgtcggccag gggcgcgtcc agcgagatag
     1681 gcgtctccgc catgctctgc agctcgacga cgcgctcctt gctgatatgc gcaaccttcg
     1741 caatctcctc agccagcgga tcacgcccta gttcctggta cagcgcgcgg gtgatgcgcc
     1801 tgatcttata aataacctcg accacgtgga ccggcacgtg gatagtgcgc gtatattcgg
     1861 cgacggcgcg actgatggcc tggcgtatcc accaggtggc gtaggtgctg aagcggaagc
     1921 cgcgcgccgg gtcaaatttc tgcgcggcgc gaatcagccc caggttgcct tcctgcacca
     1981 ggtcgagcag cgagataccg tgcgtgttgt accgcttcgc gatactgacg accagccgca
     2041 ggttggcttc gaccagccgc tgctgcgctt ccatatcgcc ttcggtcacg cgccaggcaa
     2101 gcgacaactc ttggtcggcg gtcaataaag ggaaacgccc aatttccgcg aggtactggc
     2161 ggatagcgtt gtctgtcgtg tggttgtctt gctcctcgtc ttccccgctt tcgtcctctt
     2221 cgtctctttc ttcctcctcg tcttccctaa aatccacctc gtgaacaatt tcatcttccg
     2281 cataataagc cgacggcttt acccgccggt attccccatg cttcaaggcc tttttcgcaa
     2341 tagctaccat tacgatccta ctccttttcc tcatccactt gacactattg tttgtgggaa
     2401 ataggcctta cgccggatac atggcatgat ccggctctat ccccccactc actatctatg
     2461 caagatatgt gccaatctac atattcaccg ccctcctcgc gctattgcat ctcttgaagc
     2521 ctcttcctgt gccgcttgaa atgtttggca tatcgtaatc accgctatcg atgttgttgc
     2581 gcaacctgga aattatttcc actttgtggg gaatattccc cacttgcgaa aagccggaaa
     2641 aagcccgttt atttgattgt ggctcctggg aagtattagc gaaaccctgt acgaaagaat
     2701 gaacgtatgc agacagatgt atggctgacc gacgtcggaa accagaatcg atgtcctatg
     2761 tgcagcgcgc cgctcaattc tgattcggaa acgtgttttg cctgtggttt c
//
LOCUS       DASZAK010001076         4073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_203317, whole genome shotgun sequence.
ACCESSION   DASZAK010001076 DASZAK010000000
VERSION     DASZAK010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203317"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..290)
                     /locus_tag="VGT44_18995"
     CDS             complement(123..290)
                     /locus_tag="VGT44_18995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582954.1"
                     /translation="MTASWHKIIQGDSREVLKEFSDGYFNLIVTSPPYADARQDHYDS
                     VKPDEYADIWR"
     gene            complement(295..597)
                     /locus_tag="VGT44_19000"
     CDS             complement(295..597)
                     /locus_tag="VGT44_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582955.1"
                     /translation="MQKGNRNVKIENATKRPYSVNTEVWLSDFFWPFFSNYSYQEIIH
                     AVLEVLLEVPKPDAMDIEIPEKLLTAFGACCAAKGLIDANGFFNDAQKLVNFFVGG"
     gene            complement(792..2777)
                     /locus_tag="VGT44_19005"
     CDS             complement(792..2777)
                     /locus_tag="VGT44_19005"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEV2582956.1"
                     /translation="MLLPQPHRRPLRKRQLRPLGSRQISPLALLEMIVMLSLLLPLLA
                     SCGATATQPPPGPVVSATDNEFTPRELDIQPGQTVTWVNNGQTAHTVTADDGSFDSGL
                     FQPGAGFKHTYTKPGRYPYYCSLHGAAGGYGMAGVIVVGGSSSASASSGFPRSQPHAP
                     GAILHVPGSFPSIQAAVNAAKPGDLVMISPRTGTDSAYHESVTVKTPDITIRGQDRNA
                     VLLDGQYKLDDGFEVVADNVTIENMTARHYTGNGFYWTGVNGYRGSYLTAYGNGDYGI
                     YAYNSVNGQFDHSLAAGQPDSGFYIGACHPCDALITDVISEDNALGFSGTNAGGNLVI
                     SDSIWRDNMGGIVPNTLDTEPKPPQDGVTIINNLVENNNNIDAPAKTLEYPSIGNGIM
                     LAGGNNNVVEDNRVSGHRYYGILVISNIDTNFWEPGGNTVMNNIVSNSGVADLALAAL
                     SAGNNCFSNNTVARTSPPFLQFTHACGSLLDRAGAGDPSVAVVVFNHFMQANLGRFQA
                     RDWKTATLPGDAIQPGMPDTTIEPQGIHTLSEGTPIDMTPAAAAVAPGLTLAGLGLAT
                     PFFEVIVGFYLYYLPLALYGAWLSVITWDVVRRDDLGTGSRLGWLAIAYLIPVLGPLA
                     YFLLGISKIQRTTRFSLAIGVPVVYLVVAAVLLFFIS"
     gene            3324..>4073
                     /locus_tag="VGT44_19010"
     CDS             3324..>4073
                     /locus_tag="VGT44_19010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HEV2582957.1"
                     /translation="MATTTQTGTSVPSSAFQLAVQKVASVDVPQGKHIALAFSGGLDS
                     SLCVKLSQNKYHASKVTAITVDVGQGDEEMQQSVNRAVELGITPIVIDAKAEFTEEWL
                     SKAILANSDYNGYPVSTSMTRQLIAGKVAKKALEIGADALMEGSTGKGNDQYRMHNVF
                     SLFAPGLNIFVPVRDFDLTRGEEQLLMEYYGVPVEELIVGGDDKTMWCRSIASGGIDL
                     ATVLPDHIWMWLVPPAKAPDRPTTVTISWLHG"
BASE COUNT          955 a         1140 c         1085 g          893 t
ORIGIN      
        1 cgtggccaga tccggacgtt cccctcgtct caacgcctct gccaccactg ctttatcgtt
       61 tcgataccat gccatcttct tcctccttta gaacttctct tctagtctat cacctctttc
      121 ccttatctcc aaatgtctgc atattcatcg ggcttgacgc tatcataatg atcttggcgc
      181 gcatcagcat aagggggaga ggtaacaatc aaattgaagt atccatctga aaattctttc
      241 aatacctcac gtgagtcgcc ctgaataatt ttatgccagg aagctgtcat cagattaccc
      301 tcctacgaaa aaattaacga gtttttgcgc atcattgaaa aaaccattgg catcgattaa
      361 tcctttcgcc gcgcaacatg ccccaaaggc agtaaggagc ttttcaggta tctcaatatc
      421 cattgcatca ggtttaggca cttccagcaa cacttccaga acagcatgga tgatttcttg
      481 atagctataa ttagaaaaga aaggccagaa gaaatctgac agccagactt ctgtgtttac
      541 tgaatatggc ctttttgtgg cattctctat cttgacgttc ctgtttcctt tttgcattgt
      601 aatgccaaaa acacatagat aaggtccaac tccacttcca aactacaatg taacatttgc
      661 tggccgaagt atattaaagc cctgttggta tgtcaagggt cggtttttcc ctgacctgac
      721 cttctcttct cattacaagt attctctttt ttaacagaaa aacggttgaa tggcctgcag
      781 cgccagtcgc atcacgagat aaaaaacaac agcacagccg ctaccacaag gtagacgact
      841 ggtacgccaa tggccaggga gaagcgggta gtgcgctgaa tcttcgatat gccaagcagg
      901 aaataggcca gcggcccaag caccgggatc agataggcga tagcgagcca gcccagacgc
      961 gagccggtgc ctagatcgtc gcggcgcact acatcccagg taatcacgct gagccacgct
     1021 ccataaagag ccagcggcag atagtagaga taaaagccca cgatgacttc aaaaaatgga
     1081 gtggcgagtc ccaggccggc cagcgtcagg ccgggagcca cagcagcggc ggcaggagtc
     1141 atatcgattg gcgtcccttc tgaaagtgta tggattccct gcggttcgat ggtcgtgtcc
     1201 ggcatgccgg gttggatcgc atcgccgggc agtgtggccg ttttccagtc gcgcgcctgg
     1261 aagcgtccca ggttggcctg catgaagtga ttgaagacca ccacagccac actggggtcg
     1321 cccgcgcccg ccctgtcaag cagtgagcca caggcgtgtg tgaactggag aaatggcggc
     1381 gacgtgcgcg caaccgtgtt gttcgagaag cagttattcc ccgccgagag agccgctagc
     1441 gcgagatcgg ccacgcccga attactcacg atattgttca ttaccgtgtt cccgcctggc
     1501 tcccaaaagt ttgtatcaat atttgaaatc accagaatgc cgtagtagcg atgtccgctg
     1561 acgcgattgt cctcaacaac gttgttgttg ccaccggcca gcatgatgcc attcccgatc
     1621 gaagggtatt ccagtgtttt ggccggggca tcgatattgt tattattttc caccaggttg
     1681 ttgatgatcg tgactccatc ctgcggtggt ttgggttcgg tgtcgagcgt gttaggcaca
     1741 atgccgccca tattgtcacg ccagatcgag tcgctaatca ccaggttccc gcccgcattt
     1801 gtgccggaaa agcccagcgc gttatcctcg gagatgacat ccgtaatcag agcatcgcag
     1861 ggatggcatg cgccaatata gaagccggaa tcaggctgtc ctgcggcaag tgaatgatcg
     1921 aattggccat tgacggaatt ataggcgtag ataccataat cgccgttgcc ataggccgtc
     1981 aggtagctgc cccggtagcc gttgacaccc gtccagtaga acccgttgcc ggtgtagtgc
     2041 ctcgccgtca tattctcaat ggtaacattg tccgccacaa cctcgaaacc atcgtcgagt
     2101 ttgtactggc cgtccaatag aacagcatta cgatcctgtc cgcgtatggt gatatccggc
     2161 gtcttaaccg tgacggactc gtgatacgcg ctatctgtac ccgtcctggg gctaatcatc
     2221 accaggtcgc ccggtttagc cgcgttcacc gcagcctgaa tggatgggaa gctgccagga
     2281 acgtgtaaga tagcgcctgg ggcgtgtggc tggctgcgcg gaaagccgct tgaagcgctc
     2341 gccgatgacg aaccgccaac aacgatcacc cccgccatac cataaccgcc agccgctcca
     2401 tgcagcgagc agtagtaggg atagcggccc ggctttgtgt aggtgtgctt gaaccctgcg
     2461 cctggctgga ataaacccga gtcgaaactg ccatcatccg cggtgacggt atgcgccgtc
     2521 tggccattat tgacccatgt gacggtctgc cccggctgaa tatccagttc tctcggcgta
     2581 aattcattgt ctgtcgccga cacgactggg ccaggcggcg gctgcgtggc tgttgctccg
     2641 cacgaagcca gcaggggcaa gaggagtgag agcatcacga tcatttccaa caaggcaagt
     2701 ggtgatattt gccgcgatcc cagcgggcga agctggcgtt tgcgtagggg acgacgatgg
     2761 ggttgtggca gaagcatcgc aatctctcct tatgtgccag gttaagatat attgccagta
     2821 tagacgctgg acaatacgaa tgcaagagct tctcttcaga ccgcgccacc cttttacttt
     2881 ctatgcactt gttgactctg cggaaatatg gtatgatttc atacacaaac acaaacgcaa
     2941 ctgtcccgcg ttgatcgaac acagtacgag gcaggccgcc gacagcgatc cgatacagag
     3001 tggaaggtcg gaatggatgc gcctcctccg aacgaattcg tgagcggctg accgaagaaa
     3061 gtcatactca ggtgtagcaa tgctcctcaa atatggcaag taggaacagg cgggtggttc
     3121 ccgcgcaggc aacaaccggt aaagagtggc ctttccaacg ttcgctgcgc cgacaatcgg
     3181 acaggaacga gcaaaaggca aacagggtgg caccacggga gcgattattt cctctcgtcc
     3241 cttcttcaat gttcaggaag gggcgggagt tttttattgg ctgtctcctt ccagttcgca
     3301 ttccgttgaa ggagaaacaa cgcatggcaa ctacgacaca aaccggtacc tccgtcccca
     3361 gtagcgcgtt ccaacttgca gtccagaaag tcgcctcggt cgatgtcccg cagggcaagc
     3421 atatcgcgct ggccttttcg ggcggcctcg actcgtcgct ctgcgtcaag ctctcgcaga
     3481 acaaatacca cgcgagcaag gtcacagcca tcaccgtcga tgtcgggcag ggcgacgagg
     3541 agatgcagca gagcgtcaac cgcgccgttg agcttggcat cacgcccatc gtgatcgacg
     3601 cgaaggcgga atttaccgag gagtggctca gcaaagcaat cctggccaac tccgactaca
     3661 acggctatcc cgtctcaacg tccatgacgc gccagttgat cgcgggcaag gtcgcgaaga
     3721 aagccctgga gatcggcgca gacgcgctga tggaaggctc aaccggtaaa ggcaacgacc
     3781 agtaccgtat gcacaacgtc ttcagcctct tcgcgccggg tctcaatatc ttcgtccccg
     3841 tgcgcgactt tgacctgacg cgcggcgaag agcagctgct gatggagtac tacggcgtgc
     3901 cggtcgaaga actgatcgtc ggcggcgacg acaaaaccat gtggtgccgc tccatcgcca
     3961 gcggcggcat cgacctggcg accgtgctgc ccgatcacat ctggatgtgg ctcgtcccac
     4021 cggccaaagc gcctgacaga cccacgaccg tcacaattag ctggctgcat ggc
//
LOCUS       DASZAK010001077         1963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_455493, whole genome shotgun sequence.
ACCESSION   DASZAK010001077 DASZAK010000000
VERSION     DASZAK010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455493"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..>1963
                     /gene="nuoL"
                     /locus_tag="VGT44_19015"
     CDS             35..>1963
                     /gene="nuoL"
                     /locus_tag="VGT44_19015"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEV2582958.1"
                     /translation="MAFYQASWLILLSPLFSFAVIIFGTRMWDLTIRHREAAATAGDA
                     ISHDAQGDQDAHAGHGEGEHAAHGLEDDEDPKVPHLTIGARASAYVGIGIMALACLYS
                     WLLLLNSAGVLSLAPPLGPNGVTVLSYDWFVQGTANYTVSFHLDNLGIAMMVVVTTIS
                     LLVQFYSQGYMENSAGYARFFAYLSLFTFSMLDIVFAQNFLVIFVGWELVGLSSYLLI
                     GFWINKTAKPEQDRPSPATAAIQAFITNRVGDVGFIIGIMILFTQTGTFDFPTLASKT
                     DPHGVIHAFAGNTTLLTIAMILIFCGAIGKSAQVPLTVWLPTAMEGPTPVSALIHAAT
                     MVAAGVYMVARTYPLFAAADPAALQVVAWVGAITALYAALCALTQTDFKRVLAFSTIS
                     QLGYMFVGLGVAAGTDYGPAVGMFHLFTHAFFKALLFLGAGSVINSLHHATHQEVQDM
                     RKMGGLAKFMPITAITWLIATLSISGFPFFAGFYSKDTIIGLAFDQGDYALWIITLGT
                     AGLTAFYMLRAFIVAFGGKGGSFGGLWGGTYRGEGTPHEAPRTITIPLVILAIASILA
                     GYWTGFFSFAQPGAPNLSLAEILSNGLTWIGVAVALIGLAWAYILYTRFDLATIHDVV
                     EGNAVLRFLHRLTLRKFYI"
BASE COUNT          337 a          654 c          550 g          422 t
ORIGIN      
        1 atagatacga tgaaggggtg aaagggaaga agacatggca ttttatcagg cttcctggct
       61 gatcttactc tcaccgctct tctctttcgc ggtgattatt ttcggaacgc gcatgtggga
      121 cctcaccatc cgccaccgcg aagcagcagc cactgctggc gacgctatct cgcatgatgc
      181 gcaaggcgac caagatgccc acgctggaca tggcgaaggc gaacatgcag cgcatgggct
      241 ggaggacgac gaggacccca aagtgccgca tttgacgata ggcgcacgag ccagcgccta
      301 cgtcggcatc ggcatcatgg cgctcgcctg cctctattcc tggctgctct tgctcaactc
      361 ggcaggcgtc ctcagcctcg cgccgccgct gggtccgaac ggcgtaacgg tgctgtccta
      421 cgactggttc gtccagggaa cagcaaacta taccgtcagc ttccacctgg ataacctggg
      481 catcgctatg atggtcgtcg tcaccaccat ctcattgctc gtgcagttct attcgcaggg
      541 ctacatggag aactctgccg gttacgcgcg cttcttcgcc tacctctcgc tcttcacctt
      601 ctcgatgcta gacatcgtct tcgcgcagaa cttcctggtg atcttcgtcg gctgggagtt
      661 ggtcggcttg agttcttacc tgttgatcgg cttctggatc aacaagacgg ccaaaccgga
      721 gcaggatcga ccgtccccgg ccaccgccgc gatccaggcg ttcatcacca accgcgtcgg
      781 cgatgtcggt ttcatcatcg gcatcatgat cctgtttact cagaccggta ctttcgattt
      841 ccccacactg gcctcaaaga ccgacccgca cggcgtgatc cacgccttcg cgggcaacac
      901 cacgctgctg accatcgcca tgatcttgat cttctgcggc gcgatcggca aatcggcgca
      961 ggtgccgctc accgtctggc tgccgaccgc tatggaaggc ccgacgcccg tcagcgcgct
     1021 gatccacgcg gctacgatgg tcgcggctgg cgtttacatg gtggcgcgca cttatcccct
     1081 cttcgcggcg gcagaccccg ccgcgctgca ggtcgtggcc tgggtcggcg cgatcaccgc
     1141 gctgtacgcc gccctgtgcg cgctgacgca gaccgacttc aagcgtgtgc tggccttctc
     1201 gaccatcagc cagcttggct acatgttcgt cggcctgggc gtggctgccg gaacagacta
     1261 cggccccgcc gtcggcatgt tccacctctt cacgcatgcc ttcttcaagg cgctgctctt
     1321 cctgggcgcg ggcagcgtca ttaattccct gcatcacgct acacatcaag aggtgcagga
     1381 catgcgcaag atgggtgggc tggcaaaatt catgcccatc accgccatca cctggctgat
     1441 cgcgacgctg agtatttccg gcttcccgtt cttcgccggc ttttatagca aggataccat
     1501 catcgggctg gccttcgacc agggcgatta cgcgctgtgg atcatcacac tcggtacagc
     1561 cggcctgaca gccttctaca tgctgcgcgc cttcatcgtg gccttcggcg gcaagggtgg
     1621 cagtttcggc gggctgtggg gcggcacgta tcgcggcgaa ggcacaccgc acgaagcgcc
     1681 gcgcactatc accatcccgc tcgtcatcct ggcgattgcc tcgatcctcg cgggctactg
     1741 gactggcttc ttcagcttcg cccagcccgg cgcgcccaat cttagcctcg cggaaatcct
     1801 ttctaacggg ctgacgtgga tcggcgtagc ggtggccctc atcggcctgg cctgggccta
     1861 catcctgtat acgcgcttcg accttgccac cattcacgat gtcgttgaag gcaacgccgt
     1921 gctgcgcttc ctgcaccgcc tgacgctgcg caagttctac atc
//
LOCUS       DASZAK010001078         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_346899, whole genome shotgun sequence.
ACCESSION   DASZAK010001078 DASZAK010000000
VERSION     DASZAK010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346899"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VGT44_19020"
     CDS             complement(<1..203)
                     /locus_tag="VGT44_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582959.1"
                     /translation="MPASPAVAQTGWAAQRLLPAAGPPVSLDVYDVRNAPFDIVLSAS
                     VASGLINRQQARIYLLSDVDAAE"
     gene            398..2008
                     /locus_tag="VGT44_19025"
     CDS             398..2008
                     /locus_tag="VGT44_19025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2582960.1"
                     /translation="MSEKMVAPELFRPQVDFPDVPYDHLLRAAASRNPDRPAMIYHDL
                     RLTYREVVSMVNSIANGLHSLGLRKGNRICLLTLNRPEYTITFIAAATMGIISSPMNP
                     AYKEREIGYQLENSEADAILIQRELLPLLRLVLSQKDLPNLKHIIVTGDGPVEGMPNA
                     IPFARLMRESSPLHPPHVDISGDDLVALPYSSGTTGFPKGTMLTHRNLTSNNLQFTIA
                     LQVDITDVALIFLPFYHIYGVMLTGSFLACGGTQVIMERFDLMQSLELSEKHQVTYYF
                     AVPPIVLALANAPVDLGKMKTVKYVFSGAAPLPMDPARKLQEKSGVTVVQGYGMTEAS
                     PLTHAQPKDPAHIRIDSVGMPVHNTEQKIVDIETGTRELPPGQDGEIIIRGPQIMTGY
                     WKAPAETANALRDGWLYTGDIGHADTDGYTYIVDRKKEMIKYKGFGIAPAELESLLME
                     HPAVMDSAVIGIPDDEAGELPKGFVVIRKGHEGVTPDDILAFANGKLAGYKKIHMVEF
                     IEAIPKVASGKILRRELKDLEKARRAAQ"
BASE COUNT          479 a          668 c          632 g          446 t
ORIGIN      
        1 aattcggcgg catccacgtc gctcaagagg tagatgcgcg cctgctgccg gtttatcaac
       61 ccgctcgcaa ccgatgcgct caacactata tcgaatggcg cgttccgcac gtcgtagaca
      121 tccaggctga ccggaggtcc ggcggctggc agcaggcgct gagcggccca accggtttgg
      181 gcaactgctg gtgaggcggg catagtgtgt ctttctagcg atgcgatgtt tataccctac
      241 gggcgtattg tatcatcttg cacttgaccg cgttacctca actgcgatat gctacatcgc
      301 aagaagcgcg accttgcata caacgaggtt gcgaatgcct gattgtttgt ccgtgtgcct
      361 gaaatgctct atgatgagtc agaaaggact ctttggcatg tctgaaaaaa tggttgcgcc
      421 tgaattgttt cgcccgcagg ttgatttccc cgatgttcct tacgatcatt tgctgcgcgc
      481 ggcggcatcg cgaaaccctg accgcccggc gatgatctac catgacttgc gtttgaccta
      541 ccgcgaagtt gtttcgatgg tgaacagcat cgccaatggt ctgcacagcc tggggctgcg
      601 caagggcaac cgcatctgcc tgctgaccct caaccggcca gaatatacca tcaccttcat
      661 cgcggcggct acgatgggca ttatctccag ccccatgaac ccggcctaca aagagcgcga
      721 aatcggctac cagcttgaaa attccgaggc tgacgccatc ctgatccagc gcgaactgct
      781 gccgctgttg cgcctggtac tttcgcagaa agacctgccc aacctcaagc atatcatcgt
      841 gacgggagac ggcccggtcg aggggatgcc gaatgccatc ccatttgccc ggctgatgcg
      901 cgagtcgtct ccgctgcacc cgccgcatgt cgatatttcc ggcgatgacc tcgtggccct
      961 gccctactct tctggcacga ccggcttccc caaaggcacc atgctgacgc accgcaatct
     1021 gaccagcaac aatctgcaat tcaccatcgc gctgcaggtt gatattacag atgtcgcgct
     1081 gatcttcctg ccgttctacc acatctacgg cgtgatgctg acgggcagct tcctggcctg
     1141 cggcggtacg caggtcatca tggaacgctt cgacttgatg cagtcgctgg agttaagcga
     1201 aaagcatcaa gtgacctact actttgccgt gccgccgatt gtgctggcac tggcaaatgc
     1261 gcctgttgac ctgggtaaga tgaagaccgt caagtacgtc ttctccggcg ctgcgcccct
     1321 gccgatggac ccggcgcgca agttgcagga gaagagcggt gtcacggtcg tgcagggcta
     1381 cggcatgact gaggcatcgc cgctgacaca cgcgcagcca aaggaccccg cccatatccg
     1441 catcgacagc gtcggtatgc cggtgcataa taccgagcag aagatcgtcg atatcgagac
     1501 ggggacacgc gaactgcccc ccggccagga tggcgagatc atcattcgcg gcccgcagat
     1561 catgacgggt tactggaaag cgcctgctga gacggccaac gccctgcgcg acggctggct
     1621 ttataccggc gatatcggcc acgccgatac cgacggctat acctacattg ttgaccgcaa
     1681 gaaggagatg attaagtaca agggcttcgg catcgccccg gcggaactcg aatcgttact
     1741 gatggagcat cctgccgtga tggattccgc cgtgatcggc attcccgacg acgaggcggg
     1801 cgaactgcct aagggcttcg tggtgatccg caaaggccat gaaggggtca cgcccgatga
     1861 cattctggcc ttcgccaacg gcaaactggc cggctacaag aagatccaca tggtcgagtt
     1921 catcgaggcc atcccaaagg tagcctccgg taaaatcttg cgccgcgaac tgaaagacct
     1981 ggagaaagcg cgccgcgccg cccaatgatg ccgattaaca aaataaccga gccatatatg
     2041 tagggacagg gagaggcagg gtggagcggc gggggccttg tgcccaatac atttcaaata
     2101 aggcccatcg gcgtcacctc cgtcagcagg gtagaccctt ggggtcgcag cagcacgacc
     2161 acgaaatcct caaaacgctc ctctggctca aagggcttcg gtgggacccc attgcgcttc
     2221 ttggg
//
LOCUS       DASZAK010001079         7775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_240094, whole genome shotgun sequence.
ACCESSION   DASZAK010001079 DASZAK010000000
VERSION     DASZAK010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240094"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VGT44_19030"
     CDS             <1..1285
                     /locus_tag="VGT44_19030"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV2582961.1"
                     /translation="WRTEPEIDEERQKYLTERRSTTPNIELGIYPFKDIKLSRADVEW
                     LLATHENGCGPIDWNDVSQRERVGLDLRGADLSFANLRGLPLASTIGGLSQAEWVRTT
                     PEQSSAAAVHLIRANLQEAHLEGANFRKAILEGAYFHKAYLKEADFRRAHLRGAYFRE
                     ADLEGSDFRLAYLQEADFRNSHLQQTIFRGAHLEEADLRGAQLAQADLKGAVLDTVTK
                     LEGVTFGNKEQGFALLAGLHWGDADLSEIDWTHIRYVGDEKEANRLRNPTGELKDSIT
                     TLNEYRKAVIANRRLAVALQSQGLNEDAARFRYRAQKLQQIVFRRQKNFGQSLFSLFL
                     DLLAGYGYKPGRSFLAYILVISIFSAVYHLLGTHLAWNEAIVISMTAFHGRGFFPDQF
                     HPGDPQALVAAIEAFVGLLIEVTFIATLTQRLFGK"
     gene            1931..2122
                     /locus_tag="VGT44_19035"
     CDS             1931..2122
                     /locus_tag="VGT44_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582962.1"
                     /translation="MNNTAAFIVAIVLGILALVVGYLYNANLYLGYHPSRAYAAWAVG
                     AVLIIVGIVGMVMARRRYQ"
     gene            2140..2331
                     /locus_tag="VGT44_19040"
     CDS             2140..2331
                     /locus_tag="VGT44_19040"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV2582963.1"
                     /translation="MAGVVLEFLVQPGDQVAVDQDVVMLESMKMQIPVQATAAGKVVA
                     LKVNAGDFVDDGDVLLEVE"
     gene            2333..3265
                     /locus_tag="VGT44_19045"
     CDS             2333..3265
                     /locus_tag="VGT44_19045"
                     /inference="COORDINATES: protein motif:HMM:NF023734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DA1"
                     /protein_id="HEV2582964.1"
                     /translation="MNRVCKLCGKALTGSYISALGADWHPDHFLCAACGLPVRDASFT
                     VKDGQPYHPQCYVERILPRCAYCNAPLVGEYLVDGWGTSFCAKHRQEYPTCDFCGRLI
                     PPSRQERGDPLAIQSKRCAVCRSKAVENWQQARPLYDEVTRWVKTQLPGFPNTAFALE
                     LCDRATLNRYISVMGTALVGDMHTLGVTRSTRQTLNGREVSTDVDGIAVLRGLPTPLF
                     QGVLAHELGHAWLITQGITGLAPWLEEGLCEMLAHRYYTHLNTPEGRYYANTIEQNPN
                     PVYGEGFRKLRATINRVGFLNYMTQLRANKHPPA"
     gene            complement(3270..5015)
                     /locus_tag="VGT44_19050"
     CDS             complement(3270..5015)
                     /locus_tag="VGT44_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582965.1"
                     /translation="MHTIKAGPLKVQSPLSVQGLCALGVLALGCGIRLALILAGWPHT
                     NFDEGTIGQMAMNIALHGEHPAFFYGQDYMGSLQAYLGAAFFHLFGVSLLSLRLGTVL
                     LDTLFLISMYALARLLYPPGMALLSTLLLGLASVMIYYREIQAIGGYPETLLFGALLF
                     TLASWLALTARQAHVSRRGQLARLLAYTAWGMAAGLGLWSNLLVLPYIFCAGLLLLVT
                     CWRDLRSLAPLFLLLGVVIGGLPLILYNFQPGSHANSLDIVLFLFHGGAGAPNYTLQR
                     LLPRSIPSAVLVGIPTMTSYPSVCSVSSTLYLGGKGPAALQCAVTQGAWSIFWIALWL
                     CATVLAILGILDARRSKADKVAGDGAETSEDTAAAAERWQAYARNCACLALLLALALT
                     VVLYVVSPASVIQPYLNARYLFCLQIGIPALIYPLWCWASLTPYDRWVNMLRVMFGRV
                     MLIALTLVLALATVLTFADIPGTLAMNAQQQDLVQRLEHIGAFHIYSDFMSCDRIEFQ
                     SYERITCSDLLPNLQPVGDRYKPNGLIVRSDPLSSYVFPVGSPQVSAVAQKFKQEGQM
                     YHEYLFDGYVVYQPE"
     gene            5259..5750
                     /locus_tag="VGT44_19055"
     CDS             5259..5750
                     /locus_tag="VGT44_19055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsinothricin resistance N-acetyltransferase
                     ArsN1 family A"
                     /protein_id="HEV2582966.1"
                     /translation="MRARTATLADAAAIAAIYNQGIEDRIATFETHPRTADDIAQWFD
                     ARHPIVVVEDEQGVIAFGSTSTYRPRECYNGIAECSVYVTRDKRHAGVGRLALQALID
                     AAEAAGFWKLVSRVFVDNRASRGLIGSLGFREVGVYEKHGKLDGAWRDVIIVERLIEA
                     NIT"
     gene            5764..6387
                     /locus_tag="VGT44_19060"
     CDS             5764..6387
                     /locus_tag="VGT44_19060"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEV2582967.1"
                     /translation="MYLPAHFREDDLPTLQAFIRENSFGILVTQREGAPEATHLPFLY
                     DAERGPYGTLIGHIALANQQWQAFDGAQEGLAIFSGPHAYITPSWYEVELSVPTWNYA
                     AIHAYGKLQRIDDPSELYALLKNLIQLHESQFEHPWTYPLPEEYVEKMMRGVVGFTMP
                     ITRLEGKYKMSQNRNENEQKRVIAELQETSPQVAQTMQEVYRRKRGG"
     gene            complement(6518..6955)
                     /locus_tag="VGT44_19065"
     CDS             complement(6518..6955)
                     /locus_tag="VGT44_19065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582968.1"
                     /translation="MRALRNGLLAGLFAGLALAMFNFVQDGAPGRTLPDALHWFGISL
                     SDGTTSKYIGFILFIVLGSIAGLVYGLLQRGASTTLPRALSLGLLIGFAWWFVLPFLL
                     NNITHHSFAFSFSDTLRSLPIDLLFGVVLGAAYFQWQGADAKA"
     gene            complement(6983..7567)
                     /locus_tag="VGT44_19070"
     CDS             complement(6983..7567)
                     /locus_tag="VGT44_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582969.1"
                     /translation="MSISSSTKSRATTVQRETQPVTLSERSLVGIAFVRVFVGYLWFQ
                     QLFWKLPPDFAGLYKYIVRESHNALIPGYGALLSHTFLAGCASPVSPAGCTLFVPLAA
                     GVWTAELLVAISLMFGLFTRFGAILSCILAAQLYFGLAYADWYWQYGMLILLGLALVA
                     IPAGRRFGIDQWLSPRLRDARDHSRLARIAAWFV"
BASE COUNT         1847 a         2140 c         2219 g         1569 t
ORIGIN      
        1 ctggcgtacc gagccagaaa ttgatgagga aaggcaaaaa tatcttactg aacggcgaag
       61 taccacgcca aatatcgagc taggcatcta cccattcaaa gatattaagt tgagtcgtgc
      121 tgatgtggaa tggctccttg caacacatga gaatggatgt gggcctattg attggaatga
      181 tgtaagtcag agggaacgtg tagggttaga tttacgaggt gcagacctta gttttgcaaa
      241 tttaagaggt ttacctttag cctcgacaat aggtggtcta agccaagcag agtgggtaag
      301 aactacccct gaacagagtt cagcagcagc agtgcattta ataagagcta atcttcaaga
      361 agcgcatctt gaaggagcta actttcgtaa ggcgatctta gaaggagcct acttccataa
      421 ggcttatctg aaagaagccg attttcgccg agcacattta cgtggtgctt actttagaga
      481 agctgattta gaaggttcgg atttccgctt agcatatctg caagaagcag attttcgtaa
      541 ttcgcatcta cagcagacca tttttcgagg agcgcattta gaagaggctg atcttcgggg
      601 ggcacaattg gctcaggcag accttaaagg cgcagtttta gataccgtaa caaaactgga
      661 aggagtcacc tttggtaata aggagcaagg ctttgctttg ctcgctggtt tacattgggg
      721 tgatgctgat ctttctgaaa tagactggac acatatcaga tatgtaggtg acgagaaaga
      781 agcaaaccga ttaagaaacc ctactgggga gttaaaggat agcatcacca cactaaacga
      841 gtatcgaaaa gcggtaattg ccaatcgaag acttgccgtt gcattgcagt ctcaaggctt
      901 aaatgaagat gcagcacggt ttagataccg tgctcaaaaa ctacaacaaa ttgtttttcg
      961 gcgacaaaaa aattttggtc aatctctttt ctcacttttt ctcgacctcc tagctggcta
     1021 tggttacaaa ccagggagaa gctttcttgc ttacatcctc gtaataagta tcttctccgc
     1081 tgtttatcat ctgcttggta ctcatcttgc atggaacgag gcaatagtaa taagcatgac
     1141 agcttttcat ggacgtggct tcttccctga ccaattccat ccaggcgacc cgcaagcact
     1201 agtagcagcc atcgaagctt ttgtaggact actgattgaa gtcacattca ttgcaacact
     1261 aacccaaagg ctgtttggga aatgatgctc attctcaaaa accacccagc ttgttgaaag
     1321 actcgaatgc acatcctcca tagtaagcgg tgatacttct ttgagaaact agtgatgctc
     1381 tgcaagaaaa ccacgcagct ttcagtaaat tacctacatt ttgagcgtaa agttgttgca
     1441 aacatgaggc ctccgagcag ctacacttta ccccgtatga tatgataatg ggcgaatata
     1501 gttgttggga cgcgaacatg aacttgaaat agtaaggcgg gtacatgtct tcgtaggtgc
     1561 cgatttatcg cgcacaccgc cgatcatgca tattgaataa atactccggc caccggaaca
     1621 gggaagacga gggcgaccgc aagggtctcc accccgctcc gctccacccc cgcccttaca
     1681 aagaacggcg agtggcgggt cgagcggctt cggagtcttt gtaagggcgg gggtggagac
     1741 ccttgcggtc gccctggggc tgcctctgcg atgtccgcat tatttattca atatgcatga
     1801 tcggcctgct ggattgccaa tcacgcccaa cggggccgat caatcggcgg tgtgcgcgat
     1861 aaatcggcac ctacgaatgt tcatcatcgt ctccccaaaa aaatttcatg aaataaggag
     1921 aatcatacca gtgaacaata cggcagcatt cattgtggca atcgtgcttg gcatcctggc
     1981 gcttgttgtg ggctatctct acaatgcgaa cctctacctc ggctatcatc cttcccgcgc
     2041 gtatgccgcc tgggccgtcg gcgcggtact catcatcgtc ggcattgtcg gcatggtcat
     2101 ggcgcgccgc aggtaccaat gaaacgcatc gtgtcgaata tggcgggcgt cgtgctggaa
     2161 tttctggttc agcccggcga tcaggttgcc gttgaccagg atgtcgtcat gctggaatcg
     2221 atgaagatgc agatcccggt acaggcgacg gcagccggga aggtcgtcgc gctcaaagtc
     2281 aatgcaggcg attttgtgga tgacggggat gtcctgctcg aagttgaata acatgaatcg
     2341 ggtttgtaaa ctgtgcggca aagcgctgac gggatcgtac attagcgcgc ttggcgcgga
     2401 ctggcatccc gatcactttc tgtgcgctgc ctgtggtttg ccggtaaggg atgccagctt
     2461 caccgttaag gatggtcagc cctaccatcc tcaatgctac gtcgagcgca ttcttccgcg
     2521 ctgcgcgtac tgcaatgcgc cgctcgtggg tgagtacctg gtcgatggct ggggaacgag
     2581 cttttgcgcg aagcaccggc aggaatatcc cacctgtgat ttctgcgggc gtctcatccc
     2641 accatcgcgg caggagcggg gcgatccgct cgctatccag agcaaacgct gcgccgtctg
     2701 ccggagcaag gcagtcgaga actggcagca ggcgcgtccg ctttacgacg aagtgacgcg
     2761 ctgggtcaaa acacaactgc ccggctttcc caacaccgcc ttcgcgttag aactctgtga
     2821 ccgcgccacg ctgaaccgct atatcagcgt gatggggacg gcgctggtcg gtgacatgca
     2881 tacgctcggc gtgacccgca gtacgcgaca aacgctcaac gggcgcgaag tcagcacgga
     2941 tgtggacggt atcgccgtgc tgcgaggtct gcccacgccg ctcttccagg gtgtactcgc
     3001 gcacgaactg ggacacgcct ggttgataac gcagggcatc accggccttg cgccctggct
     3061 ggaggagggc ctgtgcgaaa tgctcgcgca tcgctactac acgcacctga acacgcccga
     3121 aggccgttat tatgccaata ctatcgagca gaatcccaat cccgtctacg gcgagggctt
     3181 ccgcaaactg cgcgccacta tcaatcgcgt cggcttcctg aactatatga cgcaactgcg
     3241 cgccaacaag catcctcctg cgtagtgctt cattccggct gatacaccac atacccatca
     3301 aatagatact cgtgatacat ctgaccctcc tgcttgaatt tttgcgcaac cgcgctgacc
     3361 tgtggcgagc caacggggaa gacgtagctt gagaggggat cggagcgcac gatgaggccg
     3421 ttcggtttat agcgatcgcc tacaggttgt agattgggta gcaagtcgct gcacgtgatg
     3481 cgttcatagc tctggaactc gatgcggtcg cagctcataa agtccgagta gatgtggaaa
     3541 gcgccgatgt gttccaggcg ctgtaccagg tcttgctgct gcgcgttcat cgccagtgtg
     3601 ccggggatgt cggcgaaggt gaggacggtt gccagggcga ggacaagcgt cagagcgatc
     3661 agcataaccc gtccaaacat caccctgagc atgttcaccc atcgatcata cggtgtgagg
     3721 ctggcccagc accagagagg atagatcaac gcgggtatgc cgatctgcaa acaaaacagg
     3781 tagcgcgcgt tcaggtaggg ttggattacg gaggcaggac tgacgacata gaggacgacc
     3841 gtcagtgcga gcgccagcag cagggccagg cacgcgcagt tgcgcgcgta tgcctgccat
     3901 cgttcagccg ccgctgccgt atcttcgctc gtctctgcac catcgcccgc gaccttgtcg
     3961 gccttgcttc tcctcgcgtc caggatgccc aggatcgcca ggacggtagc gcagagccac
     4021 agcgcgatcc agaagatcga ccacgctccc tgcgtcacag cgcactgcag cgcggctggc
     4081 cctttgccgc ccaggtacag cgttgatgag actgagcaga cgctgggata gctggtcatg
     4141 gttgggatgc cgaccagcac cgcgctgggg atgctgcggg gcaacaggcg ttgcagcgta
     4201 tagttgggcg cgcctgcgcc gccgtgaaag aggaacagca cgatatcgag cgaattggca
     4261 tggctgcccg gctgaaagtt atagaggatc agcggcagtc cgccgatgac gacgcccagc
     4321 agcaggaaca gcggcgcgag cgagcgcagg tcgcgccagc acgtgacgag cagcagcaat
     4381 cctgcgcaaa agatgtaggg cagcacgagc agattgctcc acaagccaag ccccgcagcc
     4441 atgccccagg ctgtatacgc cagcagccgc gccaattgac cgcgccgcga aacatgcgct
     4501 tgccgcgcgg tcagcgccag ccagcttgcc agcgtgaaga gcagcgcgcc aaagagcagc
     4561 gtttcggggt agccgccgat ggcctggatc tcacgatagt agatcatcac cgaagctagc
     4621 ccgagcagga gcgttgagag cagcgccatg ccgggaggat agagcaggcg tgccagcgcg
     4681 tacatgctga taagaaacag ggtatcgagc aacaccgtcc ccaggcgcag cgagaggagc
     4741 gagacaccga agaggtgaaa aaatgccgcg ccgagatagg cttgcagcga acccatgtaa
     4801 tcctgcccat aaaagaaggc cgggtgttcg ccgtgtaaag cgatgttcat ggccatctgc
     4861 ccgatggtgc cttcgtcaaa atttgtatgc ggccatcccg ccaggatcaa ggccaggcgg
     4921 atgccgcacc ccagcgcgag gacacccagc gcgcaaagac cctgcacact cagcggcgat
     4981 tgtactttca gcggcccggc tttgatggta tgcatcgggt tgacacctta attgcctaaa
     5041 tcggcaccta cggattactc gattaattcg taaaaattca taattgcgcc cctacaacgg
     5101 ttcatgcacc ccggaaatac taccagaaaa catgctctca atacaaatta taggaggtat
     5161 gggcgtccgt tcctgattgg ctctctcacg catacgcgcc tggctgtggc aatcgccgcc
     5221 tccggttgtg tacaatagtc gtttaggagg atatgtaaat gcgcgcgcgc acggctacac
     5281 tggcggacgc ggctgccatt gccgcgatct acaaccaggg catcgaagac cgtatcgcta
     5341 cctttgagac gcacccgcgc acggcggatg atatcgcgca gtggttcgat gcgcggcatc
     5401 ctatcgtggt tgtcgaagac gagcaaggcg tgattgcgtt tggctcgacc tccacgtatc
     5461 gcccgcgcga atgttataac ggcatcgccg aatgctccgt ctatgtcaca cgcgacaaac
     5521 gtcacgccgg tgtgggacgc ctggcattgc aggcgctcat cgacgcggct gaagctgccg
     5581 gtttctggaa gctcgtctcg cgcgtctttg tcgataaccg cgccagtcgc gggctgatcg
     5641 gctcgctcgg cttccgcgaa gtgggcgtct acgaaaagca cggcaaactc gacggcgcct
     5701 ggcgcgatgt cattatcgtc gaacggctca tagaggcaaa tatcacctga agggaccaac
     5761 atcatgtacc taccggcgca tttccgcgaa gacgatctgc ccaccctgca agcctttata
     5821 cgcgagaaca gcttcggcat cctggtgacg cagcgcgaag gcgcgcccga agccacgcac
     5881 ctgccgtttc tctacgacgc ggagcgtggt ccatatggga cgttgatcgg ccatatcgct
     5941 ctagccaatc agcagtggca ggcatttgac ggcgcgcagg aggggctggc gatcttttcc
     6001 ggccctcacg cctacattac cccttcctgg tatgaagtcg agttgagcgt cccaacgtgg
     6061 aactatgccg ccattcacgc ctatggcaag ctacaacgta tcgacgatcc ctctgaactc
     6121 tacgccctgc tcaaaaacct tatccagctt cacgaatcgc aattcgagca tccctggacc
     6181 tatcccctgc cggaggagta tgtcgagaaa atgatgcgag gtgtggtcgg cttcaccatg
     6241 ccgatcacac gcctcgaagg aaaatacaag atgagccaga accgcaacga aaacgagcag
     6301 aagcgcgtca tagccgagtt gcaggagacc agcccgcagg tggctcaaac gatgcaggag
     6361 gtgtataggc gaaaacgggg aggatgagat atgaagagcg accgtatgta taggcgcaag
     6421 caggggaatg agatacgaag ggcgagcaca ggcatggcga cccttgcggt cgccatgtgc
     6481 gcggtcgcca tgtgtgcggt ggtggttgcc atgcccctta cgcctttgca tcagctccct
     6541 gccattggaa ataggccgcg ccgagcacga cgccgaagag caggtcgata ggcaatgatc
     6601 gcagcgtgtc gctgaacgaa aatgcgaagg agtgatgcgt gatgttgttg agcaggaacg
     6661 gcagcacgaa ccaccaggcg aagccgatca gcaggccaag cgatagggcg cgtgggagcg
     6721 tcgtggaggc gccgcgttgc aggaggccat agaccaggcc ggcgatgctg ccgagtacga
     6781 tgaacaggat aaatcctata tatttgctcg ttgtgccatc gctcaggctg atgccgaacc
     6841 agtgcagcgc atcgggcagt gtgcgccctg gcgcaccatc ctgcacaaag ttgaacatgg
     6901 ccagcgccaa tcctgcgaat aaccccgcca gcaatccatt tcgtaaggcg cgcatagtgc
     6961 tatcatctcc ttccatcttg catcagacga accaggcggc gatgcgcgcc aggcgagaat
     7021 ggtcgcgtgc gtcgcgcagg cgcggtgaga gccattgatc gatgccgaac ctgcggccag
     7081 ccgggatcgc gaccagcgcc aggccaagca gaatcagcat gccatattgc cagtaccagt
     7141 ccgcatatgc caggccaaag tataactgcg cggccaggat acacgataag atggcgccga
     7201 agcgggtgaa caggccaaac atcaagctga tcgccaccag caattcagcc gtccagactc
     7261 ccgccgccag cggcacgaac agcgtacatc ccgcgggact gacggggctg gcgcagcccg
     7321 ccagaaaggt atgcgataga agcgctccat agccggggat cagggcgtta tgcgactcgc
     7381 gcactatata cttatatagc ccggcaaagt ccggcggcag cttccagaac agttgctgga
     7441 accacaggta gcccacaaag acgcgaacga aggctatccc aaccaggctg cgctcgctca
     7501 gcgtcaccgg ctgcgtttcg cgctgcaccg tcgtcgcgcg gctctttgtg gaagaagaaa
     7561 tgctcatcga gatactactc cccttgcaac ttgtcgggac aaggatacaa taatacaggg
     7621 tataccgttg gtaacacgcg cgtgaggcca gttttttacg ttgaaaaagg cggtgtcctc
     7681 ctgcgtgtaa ttccgtaggt gccgataatg gggtctaaca aaataaagcg gcttcccgat
     7741 cagggcaacg agggcgaccg caagggtctc caccc
//
LOCUS       DASZAK010001080         6012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_203457, whole genome shotgun sequence.
ACCESSION   DASZAK010001080 DASZAK010000000
VERSION     DASZAK010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6012
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203457"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..660)
                     /locus_tag="VGT44_19075"
     CDS             complement(283..660)
                     /locus_tag="VGT44_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV2582970.1"
                     /translation="MALQTGMTGEATTTVVHENTAAAVGAGGVEVFATPMMIALMVNA
                     AWRAVEKALEAGYVTVGTLVNVYHLAATPLGQQVRATATLSEIDGRRLVFTVEAYDEH
                     QKVGEGKHERYIVNLERFLQRLG"
     gene            869..1630
                     /locus_tag="VGT44_19080"
     CDS             869..1630
                     /locus_tag="VGT44_19080"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEV2582971.1"
                     /translation="MPDHVQLVAHRGGSALAPENTLAAFRNALRYPIDAVELDVQMSR
                     DGQVIVFHDNTLERLTNGSGNILDHDFADLRSLNAAAHFPGGWPAPQQMPTLREVFEL
                     VKGRAQVYIEIKTSKRDGVTGRYPHLVESVIADVRAAYMLGQVYIISFDWTVLPEIKA
                     MEPVLPVGALVSKDAWDPQKAPFGVLAELVKSLGFEWLNMDFKLYAPDMPAIVHANGL
                     KIGLWTVNTLDDLRNCAADGVDSLTSDHPDLFVLL"
     gene            1852..2865
                     /locus_tag="VGT44_19085"
     CDS             1852..2865
                     /locus_tag="VGT44_19085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2582972.1"
                     /translation="MQQTRLPTTLIEPETLPAGEQAQQVALSIVIPVMNEDQSVRPLY
                     EKLAEQLESLGKNYEVIFVDDGSTDATFAELTKLHDEYPGVVRVIRFRRNFGKTPALV
                     AGFSRSRGEVVFTMDGDLQDDPQEIPRYLEQIEAGYDVVSGWKFPRLDPLSKTFPSRI
                     FNGLVSRTTGVHLHDINNGFKAYRREVIEDTHLKLYGEFHRFVPVMAHWRGYRVTEIK
                     VKHHPRQFGVSKFGARRFARGLIDLLNVLFLTTFLRMPLRFFGPLGFWTFLLGILVDV
                     YVVVRSVYFIHEPIHNQPLLFVGILLMIFGIQFILTGLQSEMSRHYAFRPDEEYSIRQ
                     ELD"
     gene            2865..3938
                     /locus_tag="VGT44_19090"
     CDS             2865..3938
                     /locus_tag="VGT44_19090"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEV2582973.1"
                     /translation="MPTSRISITPAMKKAAKRLIQIGLPLLILVFFFIQVKNNWGQLT
                     AHSFQWNPWLLALGFVGFLLQELSFGIIWRAVLLRLGYRLSLRVSLRIYLSSEFVRYI
                     PGNIWHVLTRILWVNKYGVPRSVALASMTIELITKLASGVLIFAVSLLFWRDITALGS
                     FAQKSIVLALGIGAIVALFVVLYPPILNGLLNLALRILKREPVSLSVRYRDILLVTLG
                     WCISWIVAGSGFYLMVLALWPSAPLVAWPICVGIYALGWDVGFVSFITPSGLGFREAA
                     ISALLALSLPISFSLAVIIALLSRFVSTIAELLCVSLAYLGGGQQQVQELQRHEQPPE
                     QRQEASEEQREMLHMVEGGVADA"
     gene            3931..>6012
                     /locus_tag="VGT44_19095"
     CDS             3931..>6012
                     /locus_tag="VGT44_19095"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEV2582974.1"
                     /translation="MPNTPWRKGFAVLRNRLYLYPPPELKPYTRWSWLALGVVALLAA
                     LYAGYFIAYMVSSHNAYLTHAEDLGTMDQAIWSILHGPVFHQTVCNIVSDTNCYSING
                     ISRFAIHFEPILFPVSLLYLLWPDAKTLLVLQTLVVAAGAFPAFWLARLRLRNDLAAV
                     AIALLYLLYPMQQQAVTFDFHAVTFTASLLLFVLYFMYTRRTIWMFAFAILAMACKEE
                     IPVVVAVYGLWSVIFQQRWRSGLGLVGLSVAWVGVYFLVEHLASPTGHSLLTPRYSDV
                     SSLLLHPKALLQQYILEPQHIQYLKLLLSPAAYLPLLAPWIFVLAVPTLALNMLSSDP
                     QQYSGLFQYNAEMVPVLIFATIEAIVLILWVLQRLFAPFYEPQRVAQPSTVETESQQN
                     EVQSGQAAQVGATTTPLPVRLLYAGVLAFLLLGITFTTAREDYLFNGKLPFSQGFQWP
                     QTTAHTQLAQKFIDEIPDDESVSAQSALLPHISHRQSVYLFPYGDEQADYIFLDISGD
                     VYPFYGSVPYTLEVKRILMSGEYGIKDAQDGYLLLEKGLPPPGISPASVTQNSKNLDV
                     VLPNLPDSLCTYIQTTPGKIVHSTLATFTSADGASSMNLVGYNVSAASPFSLAAGYMQ
                     VTTDWQLLTPTMPSLLLQVLVADESGAEHLITTDFPAQFWCPTNTWKPGMILQLSSML
                     FQIKGHSLKIGD"
BASE COUNT         1195 a         1849 c         1568 g         1400 t
ORIGIN      
        1 cgtggccaga tccggacgtt cccctcgtct caacgcctct gccaccactg ctttatcgtt
       61 tcgataccat gccatcttct tcctccttta gaacttctct tctagtctat cacctctttc
      121 ccttatctcc aaatgtctgc agtttgtaaa ccacccttgc tgcttccaac cagcatatca
      181 atcgccagtt taatgagcca gatcacgcaa gtaataacga cagatgccag gacaagaaag
      241 atcactaatg cgaggatgac gttcatacta tcacgcggct acttatccta atctttgcag
      301 aaaccgttcc aggttgacga tgtagcgttc gtgtttgccc tcgcccactt tctggtgttc
      361 gtcgtaggct tccaccgtaa agaccaggcg gcggccgtcg atctcagaga gcgtggccgt
      421 ggcgcgcacc tgctggccga gcggcgtcgc agccaggtgg taaacattga ccagcgttcc
      481 gaccgtcaca taccctgcct ccagcgcctt ctccacagcg cgccatgccg cgttcaccat
      541 cagcgcaatc atcatgggcg tcgcgaagac ctccacgccg cccgcgccaa ccgccgccgc
      601 cgtgttttca tgcacaaccg tggtggtcgc ctcgcccgtc atacctgtct gcaatgccat
      661 atgtatctcc cctttgtttg aagtagtgtg gaaatactat acaacaaaat tgccccggca
      721 tggcgcaccc ttgcggtcgc catgcctcca atccctcata atcacactat gatacaactg
      781 atacaataga tacaatcagc ctgcttgcag acatggtatc atgaaataga gaaaccaaca
      841 catgcctgga gaggacaatc atacagcgat gccagatcac gtgcaacttg tggcgcaccg
      901 gggcggttcc gcgctcgcgc cggagaatac cctggcagcc tttcgcaatg ccctgcgtta
      961 ccccatcgat gccgtcgaac tggacgtgca gatgagccgc gacggacagg tgatcgtttt
     1021 tcacgacaat accctcgaac gactgacgaa cggctccggc aatatcctcg atcacgactt
     1081 tgccgacctg cgcagcctca acgccgccgc gcactttccc ggcggctggc ccgcaccgca
     1141 gcagatgccg acgttgcgcg aggtgtttga actggtgaag ggccgcgcgc aggtctatat
     1201 cgagatcaag accagcaagc gcgacggcgt gaccgggcgc tatccgcatc tcgtcgagtc
     1261 ggtcatcgct gatgtgcgag cagcgtacat gctcggccag gtctacatca tctcgttcga
     1321 ctggacggtc ttgccagaga ttaaagccat ggagccggtc ctgccggtcg gcgcactggt
     1381 ctcaaaagat gcatgggacc cgcaaaaagc gccgtttggc gtgctggcgg agctggtgaa
     1441 gtcgctgggc tttgaatggc tcaacatgga ttttaaactg tatgcgcccg atatgcccgc
     1501 cattgtccac gcgaacggct tgaagatcgg tctgtggacg gtcaacacgc tggacgacct
     1561 gcggaactgc gctgctgacg gcgtcgattc gctgacctcc gatcaccccg atctgtttgt
     1621 cctcctatag ttcctatctg cgtgtctttt tcccctcgta acccgttata tctctagtga
     1681 gcagatgaat gtagcatgag aaggtaccta agtaccttct cttaacgttt ttcatgtggc
     1741 ctatgttata attcgctgca atcgcaagcg gcagcgaaag agagacatgc agcgcaagaa
     1801 cctataacga gaaagacatc ctcccggtga tgtatgtgga gatgatctga tatgcagcaa
     1861 acccgattgc caacaacatt gatcgagccg gaaacccttc ctgctggcga gcaagcgcag
     1921 caggtggcgc tctccatcgt gattccggtc atgaatgagg atcaaagtgt gcgcccgctc
     1981 tatgaaaaat tggcggaaca gcttgagagc ctgggcaaga actatgaagt catctttgta
     2041 gatgatggca gcaccgatgc caccttcgct gaactgacaa agttgcatga cgaatacccc
     2101 ggcgtcgtgc gcgtgatccg cttccggcgc aacttcggca aaacccccgc gctggtggcg
     2161 ggcttcagcc gcagccgtgg cgaagtcgtc tttacgatgg acggcgacct gcaggatgac
     2221 ccgcaggaaa taccgcgcta cctggaacaa atcgaggctg gctatgatgt cgtctccggc
     2281 tggaaattcc cgcgcctgga cccactctcc aagaccttcc cctcgcgcat cttcaacggg
     2341 ctggtcagtc gcacgaccgg cgttcacctg cacgatatca acaacggctt caaagcctat
     2401 cgtcgtgaag tcatcgagga tactcatctc aaactctacg gcgaattcca ccgcttcgtg
     2461 ccggtcatgg cgcactggcg gggctatcgc gtcaccgaga tcaaagtgaa acaccaccca
     2521 cgccaattcg gcgtctccaa atttggcgca agacgtttcg cgcgcggcct gatcgatctc
     2581 ctgaacgttc tcttcctgac gaccttctta cgcatgccac tgcgtttctt cggcccactg
     2641 gggttctgga cgttcttact tggcatactg gtagatgtct acgtggtggt gcgcagcgtc
     2701 tacttcattc acgagccgat tcataaccag cccctgctgt ttgtcggtat tctgctcatg
     2761 atcttcggca tccagtttat cctcacgggc ttacagagcg aaatgagtcg gcattatgct
     2821 ttccgacccg atgaagagta cagtatcagg caggaacttg attgatgcct acgtctcgca
     2881 tatcgataac acctgcgatg aagaaagccg cgaaacggct cattcagatc ggtctgcccc
     2941 tgctgatctt agtctttttc ttcatccagg taaagaataa ctggggccag ttgaccgcgc
     3001 atagctttca atggaatccc tggctgctcg cgctgggctt tgtcggcttt ctgctgcaag
     3061 aactctcgtt tgggatcatc tggcgggcgg tgctgctgcg cctgggctac cggttgtccc
     3121 tgcgagtcag cctgcgcatc tacctgtctt ccgagtttgt gcgctacatt cccggcaaca
     3181 tctggcacgt cctcacgcgc attctgtggg tgaacaagta cggcgtacca cgctccgtgg
     3241 cgcttgccag catgaccatc gaattaatca ccaagctggc gtcgggcgtg ctgatcttcg
     3301 cggtcagcct gctcttctgg cgcgatatta ccgcacttgg ctcattcgcg caaaaaagca
     3361 tcgtcctggc gctcggcatc ggcgccatcg tcgccctctt tgtggtgctc tatcctccca
     3421 tcttgaatgg cctgttgaac ctcgccctgc gcatcctgaa acgcgaaccg gtcagcctct
     3481 cggtgcgcta ccgcgatatc ctgcttgtga cgctcggttg gtgcatcagt tggatagtgg
     3541 cgggaagtgg tttctacctg atggtgttgg cgctctggcc gtccgcgccg ctcgtcgcgt
     3601 ggcccatctg cgtcggcatc tatgccctgg gctgggatgt cggctttgtc agcttcatca
     3661 ccccaagcgg cctcggtttc cgcgaagcgg ccatcagcgc gctgcttgcg ttgtcgctgc
     3721 cgatctcgtt ctctctggcg gtcatcatcg ccttgctgtc gcgcttcgtt tccaccatag
     3781 ccgaattgct ctgcgtcagc ctcgcctacc tgggcggcgg acaacaacag gtgcaggagc
     3841 tacagcggca tgaacaaccg ccagaacaac ggcaagaagc gtcggaagag cagcgcgaga
     3901 tgcttcacat ggtggaagga ggggtcgcag atgcctaaca caccctggcg caaaggcttt
     3961 gccgttctgc gcaatcgcct ctatctttac cctccgccgg agttgaaacc ctatacaagg
     4021 tggtcatggc tcgcgctcgg tgtggtagcc ctgctggcgg cgctctacgc gggatatttc
     4081 atcgcctaca tggtgtcgtc ccataacgct tacctgaccc acgcagaaga cctggggacc
     4141 atggaccagg ccatctggag tattttacat gggccggtgt tccatcaaac cgtctgcaac
     4201 atcgtcagcg atacgaattg ttacagcatc aatggcattt cgcgcttcgc catccatttc
     4261 gagccaatcc tgttccccgt ttcgctgctt tacctgctct ggccggatgc gaaaacgctg
     4321 ctcgtgctgc aaacgctggt cgtggcggcg ggtgcattcc cggccttctg gctggctcgc
     4381 ctgcgcctgc gcaacgacct cgccgcagtc gccatagccc tgctctacct gctctatccc
     4441 atgcagcaac aggcggtcac gttcgatttc cacgccgtca cttttaccgc gtccctgctg
     4501 ctcttcgtgc tgtatttcat gtatacccgt cgcacgatct ggatgttcgc cttcgccatt
     4561 ctcgccatgg cctgcaaaga ggaaattccc gttgtggtgg ccgtgtatgg cctctggtcg
     4621 gtgatcttcc agcagcgctg gcgttccggc ctgggattgg ttggcctgag cgttgcctgg
     4681 gtgggcgtct atttcctggt ggagcatctc gccagtccga ccggtcattc gctgctgacg
     4741 ccacgctata gcgatgtctc ttccctgctg cttcatccca aggccttgct ccagcagtac
     4801 atcctggaac cgcaacatat ccagtatttg aaactgctgc tctcgcccgc cgcgtacctg
     4861 ccgctgctgg ctccctggat ttttgtcctc gcggtgccga cgctcgcgct caacatgctg
     4921 agttctgatc cccagcagta ctctggcctc ttccagtata acgcggagat ggtgccagtc
     4981 ctgatttttg ccaccatcga ggccattgtg ctgatcctgt gggtcttgca gcgcctcttt
     5041 gccccgttct acgagccaca gcgcgttgct cagccatcga cagttgaaac agagtcccaa
     5101 caaaacgaag tgcagagcgg tcaagcggca caggttggag caaccacaac cccgctgccg
     5161 gtgcggctgc tctacgctgg agtgctggcc tttttactgc tgggcattac ctttaccacg
     5221 gcgcgcgaag attacctgtt caacggcaag ttgcctttct cgcagggctt tcagtggccg
     5281 cagacgacgg cgcacacgca actggcccag aagttcatcg atgagatccc agacgacgaa
     5341 tcggtctcgg cgcaatccgc gctcttgccc catatcagcc atcgccaatc ggtctatctt
     5401 ttcccatacg gagatgagca ggccgactat atctttctgg atatcagcgg agatgtctac
     5461 cccttctatg gctcggttcc ctatacactc gaagtgaaac gcatcctgat gagtggggag
     5521 tatggcatta aagatgctca ggacggctat ctcctgctag aaaagggtct gcccccgccg
     5581 ggcatttcgc ctgcctcggt aacgcagaac agtaagaacc tggatgtcgt gctgcccaat
     5641 ttgccggaca gcctctgtac ctatattcaa accacgcctg gaaagatcgt ccattcgacc
     5701 ctggccacct tcaccagcgc cgacggtgcg agcagcatga acctggtggg ctacaatgtg
     5761 tcagccgcca gcccgttcag cctggcagca ggctatatgc aggtcacgac cgattggcag
     5821 ctcctgacgc cgaccatgcc gtcgctgctc ctacaagtac tcgtagcaga cgaaagcggc
     5881 gctgaacacc tgatcaccac tgacttcccg gcccagttct ggtgtcctac caacacctgg
     5941 aagcccggca tgatcttaca gctatccagc atgttattcc agattaaagg gcatagtttg
     6001 aagataggag at
//
LOCUS       DASZAK010001081         1986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_668172, whole genome shotgun sequence.
ACCESSION   DASZAK010001081 DASZAK010000000
VERSION     DASZAK010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668172"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1986)
                     /locus_tag="VGT44_19100"
     CDS             complement(<1..>1986)
                     /locus_tag="VGT44_19100"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2582975.1"
                     /translation="TALRIALYHLRHTLEVGTGPEHTPHLLIQHEMLGLDPGSSIDLD
                     LHALREAWSLARNRTAREEAQGEARRAMIDRLQHAVALYRGSFLEDFTLRDTVDFDNW
                     MGIQRNYWYQRIEQIFDWLSQLLSVEGAFERAIATVERWRSLDPLNEDVSLRLMQLQL
                     ASGNRVAALKTYESTVEVLMEELSAKPSPKLAALAEFIRNAPAHRVALKRKQTGPSSS
                     RSLLAVPFVGREVEMGMLMSLYEKASRGQPQVVVIEGEAGIGKSRLAATFLDWARAQG
                     ADVLAGRAFHSHQHLAYQPLVDPLRERLAREPDLRRLLSDTWLAELSRLLPDLRERYP
                     DLPPASVDEVFEAPRFLEALGRLDQALAGRATLLVFIDDLQWTDRATLDVFQYLGMQW
                     AKRSTPVMVLLSRRVETRSADPWLNEWFARLKNDLPVTRLGLRPLSAKDTLQIVRSVS
                     GEDSEQDDGQSQHSTLPSTSRAERAASGETGAERLSRWLYAETKGQPFFVRTTLEAML
                     RRGVLVPRLVKGRGWVFEPQEAALRPEVLGSILPSDVREAIQRRLAQLSPPARELLVA
                     GAVLDHDFTFEFLSQVAQLTTQEALTALDEALESLLLQESQRHSEGREVPAYTFAHDK
                     IREVVYAGAGNARSRIFHARAVEVLERAGASAAELAY"
BASE COUNT          366 a          615 c          631 g          374 t
ORIGIN      
        1 ggtaggcgag ttcggcggcg gatgcgcccg cccgctccag tacctcgacg gcgcgcgcgt
       61 ggaaaatgcg gctgcgggca ttgccggccc cggcgtacac gacctcgcgt atcttgtcgt
      121 gggcaaatgt gtacgccgga acctcccggc cttcactgtg acgctgagac tcttgcagga
      181 gcagactctc cagggcttca tccagggcgg tgagtgcctc ttgcgttgtc aactgtgcaa
      241 cctggctgag aaactcgaag gtgaagtcgt ggtcgaggac cgcgcctgcc accagcagtt
      301 cacgagcagg gggagagagc tgcgccaggc gccgctggat ggcctcgcga acatcggatg
      361 gcaggatgga gccgagcacc tcgggacgaa gggcggcctc ttgtggctcg aacacccagc
      421 ctctcccttt taccaggcga ggcaccagca ccccgcgccg gagcatggct tcaagcgtcg
      481 ttctcacaaa gaacggctgt cccttcgttt cggcatagag ccacctgcta agtcgctcgg
      541 cgcctgtctc tccggacgcc gcccgttccg cgcgtgaggt ggatggaagc gttgaatgct
      601 ggctctgtcc atcgtcctgc tcgctatcct caccggagac ggagcgcacg atttgcaggg
      661 tgtctttcgc ggagagcggc ctgagtccca ggcgcgtcac ggggagatcg tttttcagcc
      721 gggcaaacca ctcgttgagc cacgggtcag cagaccgtgt ctccactcgc ctgctcagca
      781 gcaccatgac gggcgtcgaa cgcttcgccc actgcattcc caggtactga aatacgtcca
      841 gggtcgccct gtcggtccat tgcaggtcgt cgatgaagac gagcagcgtt gctcgcccgg
      901 cgagagcctg gtcgagccgc cccagcgctt ctaagaaacg aggggcttcg aaaacctcgt
      961 ctacgctggc aggaggcagg tcgggatagc gctcgcgtag atcggggagc agccgggaaa
     1021 gctcggccag ccaggtgtcg ctcaacaggc ggcgcaggtc cggctcacgc gctaaacgct
     1081 cgcgcaaagg atcgacgagt ggctgatagg ccaggtgttg atggctatga aaggccctcc
     1141 ccgcgagtac gtcggctccc tgtgcccttg cccagtcgag aaatgtggca gccaggcgcg
     1201 atttgcctat gccggcctcc ccttcgatca ccacaacctg gggctgtccg cgagacgcct
     1261 tctcgtagag cgacatgagc atgcccatct ccacctctct tccgacgaag gggaccgcca
     1321 gcaatgagcg cgaagaggaa ggccctgtct gcttgcgttt aagcgcgaca cggtgagccg
     1381 gtgcattgcg gataaattct gccagggctg ccagcttcgg cgagggtttg gcggagagtt
     1441 cctccatcaa gacctctaca gtggactcgt aggtcttcag cgccgcgacg cggttgccgc
     1501 tggcgagctg gagctgcatc aggcgcaggg agacgtcttc gttgagcgga tcgagcgagc
     1561 gccagcgctc gacggttgcg atagctcgct cgaacgcacc ctccacgctc agcagttgcg
     1621 agagccagtc gaagatctgc tcaatacgct ggtaccagta gttcctttgt attcccatcc
     1681 agttatcgaa gtcgactgtg tcgcgcaagg tgaaatcttc gagaaagctg ccgcgataca
     1741 gtgccaccgc gtgctggaga cgatcaatca tggcgcgtcg agcctcaccc tgcgcctctt
     1801 cacgtgcagt tcggttgcgc gccaggctcc atgcctccct gagcgcgtgc agatcgaggt
     1861 cgatactaga acctggatcg aggccgagca tctcatgctg aatgagcagg tggggtgtgt
     1921 gttcaggccc ggtgcccacc tcaagcgtat ggcgcagatg atataaagct atgcgcagcg
     1981 ccgttc
//
LOCUS       DASZAK010001082         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_418858, whole genome shotgun sequence.
ACCESSION   DASZAK010001082 DASZAK010000000
VERSION     DASZAK010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418858"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(27..683)
                     /locus_tag="VGT44_19105"
     CDS             complement(27..683)
                     /locus_tag="VGT44_19105"
                     /inference="COORDINATES: protein motif:HMM:NF039470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6483 family protein"
                     /protein_id="HEV2582976.1"
                     /translation="MTQRDYILRIAEDIGRALAQVLYNQPKQNYQGAHDFIDEQFRQM
                     LGMGAGFVHTAPEETLLAMLTSMGELDIEKCWLLATLLKADGDAYEAQENENESYYSY
                     LKALNLYLAVLTGEREHRRLDPVAEVEGLLYKLASYELPVRTKTLLFRYYEQTGNFAS
                     TESTLQELLEVDPDNQEIIENGRDFYRRLLRLDQASLAAGGLTRDGVEDGLMKLMLLE
                     "
     gene            complement(680..2212)
                     /gene="gltX"
                     /locus_tag="VGT44_19110"
     CDS             complement(680..2212)
                     /gene="gltX"
                     /locus_tag="VGT44_19110"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV2582977.1"
                     /translation="MTQTDHRLEEESTVAREKTPRLRFAPSPTGFLHIGGFRTALFSW
                     LYARHTGGKIVLRIEDTDVARTVEGAVEDIIEGFAWLGMDFDEGPVVGGPFGPYYQTQ
                     RRALYQQYAHQLIESGHAYRCYCTPERLARVRQEQMARKQPPRYDRHCRYLSEEERAA
                     NDAAGLTWTVRFAMPLDGETVVHDELHGDITFKNTDVDDFIVLKTDGLPTYHLAHIVD
                     DHLMEITHLLRAEEWISSAPRHIHIWNALGWEIPLLYHVPDVLGKDKRKLSKRHNAPA
                     WKDFRDQGYLPEAVFNFMALIGWSYDDKTELFTREELIKVFTLDRIGVAGGIYDAEKL
                     AWMNGVYIRKLSLEEFTRRALPYMERPEAQGGLPDSITRPLDFDETSRILALEQERLR
                     TLGEAAHAVSFFYTDEIEYETHLLIQKGMDAERTRAALLRARDLLNGLDAWEHDTMEL
                     AMRALAEELGLKPGQLFGAVRVAVSGRTATPPLFAMMEALGRERTMRRIEQAIGRLEQ
                     QS"
     gene            2303..>2549
                     /locus_tag="VGT44_19115"
     CDS             2303..>2549
                     /locus_tag="VGT44_19115"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2582978.1"
                     /translation="MSQSPEIVVTRDGPVTIIKLNRPHVRNAVNKATADALREAWLAF
                     DADESALVGILTGGDKVFCAGADLRDLEGLASGDAGKH"
BASE COUNT          493 a          786 c          732 g          538 t
ORIGIN      
        1 atgccaccgc tacggttgtc acgctgctat tccaaaagca tcaacttcat caacccatcc
       61 tcgacgccat cgcgtgtcag gccgcccgcc gccaggctcg cttgatccag ccgcagcaat
      121 ctccggtaga agtctcgtcc gttctcgatg atttcttgat tgtcgggatc gacttcgagc
      181 agttcttgca gggtgctttc cgtgcttgca aagttgcccg tttgctcgta gtagcggaag
      241 agcagcgtct tcgtgcgaac aggcaattcg tagctggcga gcttgtacag cagtccctcc
      301 acctcagcga ccgggtcgag ccggcgatgt tcacgctcgc cggtcaggac cgcgaggtac
      361 agattgagcg ctttgagata gctatagtaa ctttcgtttt cgttttcctg cgcctcgtaa
      421 gcgtccccgt cagccttgag cagcgttgcc agcagccagc acttctcgat atcgagttct
      481 cccatcgagg tcagcatcgc cagaagcgtc tcctccggcg cggtatgcac aaatcccgca
      541 cccataccga gcatttgccg gaattgctcg tcgatgaagt catgagcgcc ctggtaattc
      601 tgctttggtt ggttgtacag gacttgcgcc agggcgcgcc cgatgtcttc ggcgatacgc
      661 aggatataat cgcgttgcgt catgattgtt gttcaagtct cccaattgct tgctcgatac
      721 gccgcatggt gcgctcgcgg ccaagcgcct ccatcatggc gaaaagcggc ggagtggcag
      781 tgcgcccgct cacagccacg cgcacagcgc caaaaagttg ccctggtttc agacccagct
      841 cttccgccaa tgcgcgcata gccagttcca tcgtgtcatg ttcccaggcg tccaggccgt
      901 tcagcaggtc gcgtgcgcgt aagagcgcgg cgcgggtgcg ctcggcatcc atacccttct
      961 ggatcagcaa gtgcgtctca tactcgatct cgtcggtgta gaagaaggag accgcgtggg
     1021 cagcctcgcc aagtgtgcgc aggcgctcct gttccagcgc gaggatgcgg ctggtctcat
     1081 cgaaatccag cgggcgcgta atgctgtcgg gcaggccacc ctgggcctcc ggtcgctcca
     1141 tatagggcag cgcgcgccgc gtgaactcct cgagcgagag tttgcgaatg tagacgccgt
     1201 tcatccaggc cagcttctcc gcgtcgtaga tgccgccggc cacgccgatg cgatccagcg
     1261 taaagacctt gataagctcc tcgcgtgtga acaactcggt cttatcgtca taggaccagc
     1321 cgatcagggc cataaaattg aagaccgcct ccggcagata gccctggtcg cggaagtctt
     1381 tccaggcggg cgcgttgtga cgcttgctga gcttgcgctt atctttgccc agcacatccg
     1441 gcacgtgata gagtagcggt atctcccagc cgagcgcgtt ccagatgtgg atatggcgcg
     1501 gcgcgctgga gatccattcc tcggctcgca ggaggtgggt gatctccatg agatgatcgt
     1561 ccacgatatg cgcgaggtga taagtcggca agccatccgt tttgagaaca atgaaatcgt
     1621 caacatcggt atttttgaag gtgatgtcgc cgtgtaattc gtcatgcacc accgtctcgc
     1681 catcaagcgg catagcaaag cgcaccgtcc aggtcaaacc agccgcgtcg ttggcagcgc
     1741 gctcctcctc gctcaggtag cggcagtggc ggtcatagcg aggcggctgc ttgcgcgcca
     1801 tttgctcctg tcgcacacgc gccagccgct cgggtgtaca gtagcagcga taagcatggc
     1861 cagactcgat caactggtgg gcgtactgct ggtagagcgc tctccgctgt gtctggtaat
     1921 agggaccgaa tggcccgcca acgaccggcc cctcatcaaa atccatgccg agccaggcga
     1981 agccctcgat gatatcctca accgctccct caaccgtgcg cgccacgtcc gtatcttcaa
     2041 tgcgcagcac aatcttgccc cccgtatgac gcgcgtacag ccagctaaac agcgccgtgc
     2101 ggaaaccgcc aatgtgcaga aaccccgtgg ggctgggagc aaagcgcagg cgcggcgttt
     2161 tctctcgcgc caccgttgac tcctcttcga gacgatgatc ggtctgtgtc ataatgtctg
     2221 cttcttctcc tcgtgcaaca taacgtgtag tgtttattgt aacatgcagg gacaagtgta
     2281 actctgacga aaggataata ccatgtccca atcacccgaa atcgttgtca cccgcgacgg
     2341 tcccgtcacc atcatcaagc tgaaccgccc gcatgtgcgc aatgccgtca acaaggccac
     2401 cgccgacgcg ctgcgcgaag cctggctggc ttttgacgct gatgaaagcg cgctggtggg
     2461 catcctgacc ggcggcgaca aggtcttctg cgccggggcc gatctgcgcg acctggaggg
     2521 gctggcgagc ggcgatgctg gcaaacatg
//
LOCUS       DASZAK010001083         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_418875, whole genome shotgun sequence.
ACCESSION   DASZAK010001083 DASZAK010000000
VERSION     DASZAK010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418875"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VGT44_19120"
     CDS             complement(<1..304)
                     /locus_tag="VGT44_19120"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV2582979.1"
                     /translation="MGDQPTAIALYASILTGLYRREKTGQGCHVSTALLAVGTWTAAT
                     LVQAALDGAEFYDNIDRKNPPNALLNSYASGREESGYPKKFGFPALFIDSDTLVGTL"
     gene            complement(310..813)
                     /locus_tag="VGT44_19125"
     CDS             complement(310..813)
                     /locus_tag="VGT44_19125"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV2582980.1"
                     /translation="MANLFSGLKMLDMATFIAGPGATTILSDFGADVIKIEVPGKGDP
                     YRLTGFFPPNPRSPKNYLWQLANRNKRSMTLNLKSPRAKDILRKLIDWADVFVINFPP
                     PTRERLGLTYEQVSAMNPRIIYADITGFGNRGPEADSPGFDITAYWARSGLMDATRNA
                     GSPPPAR"
     gene            complement(945..1778)
                     /gene="phaZ"
                     /locus_tag="VGT44_19130"
     CDS             complement(945..1778)
                     /gene="phaZ"
                     /locus_tag="VGT44_19130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HEV2582981.1"
                     /translation="MASETTRPQAETRTITARGQRLRVAIRKGDGTRTPLLLMNGLGV
                     NLEVLQPFVDALDPAYEVIRFDVPGTGGSPAPRIPYRFSAHARLVREMLDQLGYQQVD
                     VLGISWGGALAQQFAYQYPMRCRRLILVSTGTGALMVPARPSVVAKMATPRRYQDPAH
                     MVQIAGEIYGGKVRTQPELAHDFARSVRSGGSRGYFYQLLGGVGWTSIRWLRKLQQPT
                     LILHGDDDPLVPLVNAKIMHRLIPNSQLYIFHDGHLGLGTSAKELAQVVDRFLTAPPL
                     K"
     gene            complement(1807..2919)
                     /locus_tag="VGT44_19135"
     CDS             complement(1807..2919)
                     /locus_tag="VGT44_19135"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEV2582982.1"
                     /translation="MYLALSDEQKMLAQTVRQFVKKELPSAKVRQIMATESAFDEPLW
                     QKMAEQGWMSLTVPVECGGLAESWLTIAVLFKQLGWGMVPGPFLATVGLAAPLLAKAG
                     SSKQKARWLPAIAEGKCRASVAYLESDASYELDAIKTTASEVGGEYTLSGTKMFALDA
                     GVADMIIVAAKEEGKLGLFLVEKGTRGLTVEPLEGMDLSRRLTRIHLKNTPAEKLDVC
                     NEPRQMLEQALNCASFALSAEGVGGMQWILDTAVAYAKSRQQYGRVIGSFQGVAYPLV
                     DRTLDLISARSAVYNAAYALDHRPDEIAEAVSMAKAHVSDSYRQAGYTGVQTLGGIGF
                     TWEHDMQLYFRRGSGTWSLFGDPNWHRERLLQAIKM"
BASE COUNT          621 a          898 c          811 g          682 t
ORIGIN      
        1 caagggtgcc gacaagcgtg tctgagtcaa taaagagtgc cgggaaaccg aacttcttcg
       61 gatatccgct ctcttcgcgt ccactggcat aggagttcag caacgcgttg gggggattct
      121 tgcgatcaat attgtcatag aattcagcgc cgtcgagggc agcctgtacc agggttgctg
      181 ccgtccacgt tcctaccgcg agcaacgcgg tagagacatg gcatccctgc cccgtcttct
      241 cacggcgata gagtccggta aggatagagg catagagagc tattgcggtg ggctgatcgc
      301 ccatacctgt tatcgggctg ggggagggct accggcgttt cgcgtagcat ccatcaatcc
      361 gcttcttgcc caataggccg taatgtcaaa gccgggactg tccgcctctg ggccacgatt
      421 cccaaaccct gtaatatccg cgtaaataat gcgcggattc atcgcggaca cctgttcata
      481 ggtcaagccg agtctctcac gcgtaggcgg cggaaaattg ataacgaaca cgtcggccca
      541 atctataagc ttgcgcagga tgtctttcgc cctagggctt ttcagattca gggtcatact
      601 tcgtttattg cgattcgcca gttgccacag gtagttcttc ggggaccgag gattcggcgg
      661 aaaaaagccg gtgagccgat acggatcacc cttaccagga acttcgattt tgatgacatc
      721 ggcaccaaaa tcggacagga tggtggttgc ccccggtccg gcgataaagg ttgccatatc
      781 cagcatcttc aggcctgaga atagattcgc catatgtttc tctccttgag aagtaaggta
      841 ttatgcccaa ttacacataa gatgaaggac ctctgcactc tctggtcata aagcgacgag
      901 agaggttgcc atgaatggtt gtccctatag aacttatgcg ctcgctactt cagtggcggt
      961 gctgtgagga agcgatcaac gacttgcgca agttccttcg cactcgtccc caagcctaga
     1021 tgtccatcat ggaagatata gagctgggaa ttggggatca ggcggtgcat gatcttcgcg
     1081 ttgacgagtg ggacaagcgg gtcgtcatcc ccatgcagaa tcagtgtcgg ctgctggagt
     1141 ttgcggagcc agcgaatgct ggtccaccct actcctccca gcagttgata gaagtatccg
     1201 cgcgagccac cagagcgaac gcttcgtgcg aaatcgtgcg ctagctcggg ttgtgtgcgt
     1261 accttccccc cgtagatttc tccagcgatc tggaccatgt gggccgggtc ctgatagcgt
     1321 cgtggcgtgg ccatcttcgc cacgaccgag ggacgggcgg gaaccatgag tgccccggtt
     1381 cccgtgctga ccaggatcag tcgccgacag cgcatggggt attgataagc aaactgctgg
     1441 gcaagcgcac caccccagga gatgcccagc acatcgacct gctggtagcc caactgatcc
     1501 agcatctccc tcaccaaccg ggcatgggcc gaaaaacggt acgggatgcg tggggccggt
     1561 gagccgccgg tgcccggcac atcaaagcga atgacctcat aggccgggtc gagggcgtcg
     1621 acgaagggct ggagcacttc gaggttgacc ccgagcccgt tcatcagcag cagcggcgtg
     1681 cgggtgccat cgcctttacg gatggcgacc cgcaggcgtt gccccctggc ggtgatggta
     1741 cgtgtctcgg cctgtggcct ggtggtttct gatgccatga atcttccttc cttactactc
     1801 cccttgctac atcttgatag cttgtaacaa gcgttcccta tgccagtttg gatctccaaa
     1861 aaggctccag gttccactgc cacgccggaa atagagttgc atatcgtgtt cccaggtgaa
     1921 gccaattcct cccagcgtct gaaccccggt gtagccagct tgccggtaac tatcgcttac
     1981 gtgcgctttt gccatgctca ctgcctcggc aatttcatct ggtcggtggt ccagggcgta
     2041 ggcggcgttg tagacggctg agcgtgcgct gatcaggtca agggtccggt ctaccaacgg
     2101 ataagccacc ccttggaaac tgccgatcac acgcccgtac tgttgacggg atttggcata
     2161 cgccacggca gtatccagaa tccactgcat cccgcccacg ccttccgcgc tcagcgcgaa
     2221 actggcgcaa ttcaaggcct gttccagcat ctgacgaggc tcattgcaga catcgagctt
     2281 ctcagccggg gtatttttca ggtggatgcg ggtcaagcgc cgactcaaat ccataccttc
     2341 caggggttcc accgtcagac ccctggtgcc cttctccacc aggaacaggc ccaactttcc
     2401 ttcctctttg gctgccacaa taatcatatc ggccactccg gcatcaaggg caaacatttt
     2461 tgtgccgctc aacgtatatt cgccgcccac ttcgcttgct gttgtcttga tcgcgtcgag
     2521 ctcatagctg gcgtcggact ccagataagc cacgctggcc cggcacttcc cctccgcaat
     2581 cgctggcagc caacgggctt tctgcttgct gctcccagcc tttgccagca gtggggccgc
     2641 cagcccgact gtagccagga acgggcccgg caccattccc cagcctaatt gcttgaacaa
     2701 gactgcaatg gttagccagg actctgctaa cccgccacat tcgaccggca ccgtcaagct
     2761 catccagccc tgctcggcca tcttctgcca cagtggctca tcaaaagcgc tttcagtcgc
     2821 cataatctgc cgcactttag cagagggcaa ctccttcttg acaaactggc gaaccgtttg
     2881 cgccagcatc ttttgctcat cgctgagagc caaatacatg tttctcgctc ctctttcaca
     2941 tgcaatcgtt tgtctcagat ccaaaggtca gcttttcgtg cttatcattg ccaactccgt
     3001 tctcagttct ca
//
LOCUS       DASZAK010001084         4480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_455579, whole genome shotgun sequence.
ACCESSION   DASZAK010001084 DASZAK010000000
VERSION     DASZAK010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455579"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1908
                     /locus_tag="VGT44_19140"
     CDS             <1..1908
                     /locus_tag="VGT44_19140"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2582983.1"
                     /translation="DLCIPQTPHLIDADTNYLTLSNYARDPSQISQSLSLVIVDIEPL
                     YRLSLHVERALEILLGLGLIIIALGVTAYTLIARTFFIRPIRRLQARVSTLVSNANSE
                     PVVDTTFDELSMLARSFNLLSESLTSQESESNAITSRMSDLLALSDILISTLNLEDLL
                     REIVSRLGIIMHTRSVSLLLYGRDMLSPWAVAQWSDTVEATSDGRVLTISGKSGAVTV
                     HTDPAGDITMAATTKMAAISLANRPDRATNSATSSPSASGKRRAIRATKLAPPPEQVG
                     QRNPRIPRPALRDLDLLLARMAIQKQKITYAEDVHTIHRERQETWTRLALEADYQSVI
                     AVPLFIQDQPIGAVMLYSNQPHMLTARDTFLLSTASIQAAMAIQNALLFAEVKNKNEA
                     LERVNRLKSQFLATVTHELRTPLHSIISYGSLLLDGYVDGELSVEQQEHLTFMVNRAE
                     LLSRLVDDMLDFSKIEADRIEVKVEPLVLARNLLEVVNELKPNASKNALYLALEVDDN
                     LPLALADSHRLRQIVTNLVSNALKFTEQGGVTIRCSVLERYDMLRISVSDTGIGISPA
                     ALDYIFESFRQADGSTTRRFGGTGLGLAIARRLIELQGGEVTVESTAGQGSTFSFTLP
                     IAARARVHASA"
     gene            1925..2335
                     /locus_tag="VGT44_19145"
     CDS             1925..2335
                     /locus_tag="VGT44_19145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2582984.1"
                     /translation="MYIQVEPGVPASGTRQPTILIVENEASNRILIERVLSTRGYRCL
                     SASNGLEALNILDIEQVDLILTDLFMPVLDGYRATQLIRARPNMAHVPIVAVTAYALN
                     DESEAALQIGCTEYLTKPFKPRQLLEVVDRLLPR"
     gene            2501..4045
                     /locus_tag="VGT44_19150"
     CDS             2501..4045
                     /locus_tag="VGT44_19150"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: protein motif:HMM:NF014165.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEV2582985.1"
                     /translation="MLLQEKTTFEFSTDDARMAELIGLAQQIEDVKALSALAEWDQNT
                     EMPEGAGEVRGNQMATIQGVLHEFRTNPRLGTLLDELETRIGQAEYTDADAGLLREMR
                     RMYDQATRLPRTLVEELARVSAGSFEAWRRARERNDFASFAPWLKRMVALQREVADRF
                     GYEETRYDALLDLYEPGMTVSKLDKLFEPVRAVSATLLRRIEASGHSVDDSCLQGDFD
                     PDKQLELGRKILKGMGYDFSRGALAVSPHPFTTGLGSPFDVRVTVLPHRDLLQASIMA
                     AIHEGGHALYEQGSAETLARTPIAGGASMGVHESQSRLWENAIGRSTAFWQGQFAAMR
                     ETFPRPYAEVDPAAFARALNKVQPSLIRIEADEVTYNLHIIVRYELEKAMVNGDVDIE
                     SLPRLWNAKYREYLGIEPQRDSEGVLQDIHWTSGFGYFPSYTLGNLYSAQIYATLRQV
                     YPDFDERLSHGDTAFILNWLREHMYTAGATYLPDTLMKQVTGEAPTPAYFVRYLTGKF
                     ERIYGL"
BASE COUNT         1027 a         1364 c         1180 g          909 t
ORIGIN      
        1 gacctgtgca ttcctcaaac gccgcatttg atagacgcgg acacgaacta tttgaccctc
       61 tcaaattacg cccgcgatcc ttctcaaatc agccaatcac tctcgctggt gatcgtagat
      121 atcgagccat tgtacaggct gagcctgcac gttgaacgcg cgctggaaat tctgctcggc
      181 ctcggcctca tcatcatcgc gctgggcgtc accgcctata cgctgatcgc tcgcacgttc
      241 ttcattcgcc cgatccgccg cctgcaagcg cgcgtctcta cactggtgtc aaacgcaaac
      301 tcggagccgg tggtagacac caccttcgac gaattgagca tgttggcacg ctcattcaac
      361 ttactctcag agtcattgac ttcccaggag agcgagagca acgccatcac cagtcgcatg
      421 agcgatctcc tggccttaag cgacatcctc atctccacgc tcaacctgga agacctgctc
      481 cgagaaattg tctcgcgcct ggggatcatc atgcatacgc gcagcgtctc actgctgctc
      541 tacgggcgcg atatgctctc gccctgggca gtggcgcaat ggtcggatac ggtggaagcc
      601 acttccgatg gtcgagtact tacaatctcc ggcaaaagcg gcgcggtaac agtccatacc
      661 gacccggcag gcgacatcac gatggcggca accaccaaaa tggcggcaat ctccctggcc
      721 aaccggcctg accgtgccac aaatagcgca acatcctcac catctgccag cggcaagcgc
      781 agagccatcc gagcgacaaa gctcgcgcct ccgcctgagc aggtcggcca gcgcaatcca
      841 cgcatcccac gtccagctct gcgcgacctc gacctgctgc tggcgcgcat ggcgatccaa
      901 aagcagaaga tcacctacgc cgaggacgta cacaccattc atcgagagcg ccaggaaacc
      961 tggacacgcc tggcattaga ggcagactac caatccgtca tcgccgtgcc gctcttcatc
     1021 caggaccagc ccatcggcgc agtcatgctt tactccaatc agccgcatat gctgactgct
     1081 cgggatacct tcctgctcag tacagcctcg atccaggcgg ccatggcgat ccagaacgcg
     1141 ctgctttttg ccgaagtgaa aaacaaaaac gaggcgctgg aacgggtaaa tcgcctcaag
     1201 tcacaatttc tggctaccgt cacccatgaa ttacgtaccc ccctgcatag cattatcagt
     1261 tatggttcgt tactcttaga tggatacgta gatggcgaac tgagcgtcga gcagcaggaa
     1321 cacctgacgt tcatggtcaa tcgcgcggaa ctactctcgc gcctggtgga cgacatgctc
     1381 gatttctcta aaatcgaggc tgaccgcatc gaggtcaagg ttgagccgct cgtactggcg
     1441 cgcaacttgt tggaagtcgt gaacgaactc aagccaaacg ccagtaaaaa cgcgctctac
     1501 ctggctctgg aggtagacga caatctgccg ttagctctgg ctgacagcca tcgtctcagg
     1561 caaattgtaa cgaacctggt ctcgaatgcc ctcaaattta ccgagcaggg cggcgtgacg
     1621 atcaggtgca gcgttctgga acgttacgat atgctgcgta tctccgtcag cgataccggc
     1681 atcggtatct caccggcggc ccttgactac atttttgagt cattccgcca ggccgacggc
     1741 agcacgacgc gccgtttcgg aggcaccggc ctcggactgg ccatcgcgcg caggttgatt
     1801 gaactgcaag gcggtgaagt gactgtggaa agcacggcgg gccagggatc gacattctcg
     1861 ttcacattgc ctatcgcagc gcgcgccaga gtacacgctt ctgcctaatc aagggaagag
     1921 agagatgtat atacaagtag agccaggtgt cccggcatcc ggcacacggc aaccaaccat
     1981 cctcatcgtc gaaaatgaag catcgaaccg cattctgatc gagcgggtcc tctccacgcg
     2041 cggctatcgc tgcctttccg cgtcgaacgg tttagaggcg ttaaacattc tggatatcga
     2101 acaggtcgat ttaatcctga ccgatctctt catgcccgtg ctggatggat accgcgccac
     2161 acagttgatt cgcgcccgcc ccaacatggc gcatgtgcca atcgtggcag tcaccgccta
     2221 cgcgctcaac gacgaaagcg aagccgccct gcaaatcggt tgtaccgagt atttgaccaa
     2281 gccctttaag ccgcgtcaat tactcgaggt cgtggaccgg ctgctgcccc gttaaaacta
     2341 tacaaaagtc acacatgttt tgcagaatgt agcacttttc tgcaaaattt gctatgattc
     2401 ttgcagctac acgtgatctc gtgatcggct cgctatggct ttccccgtcg caggaacccc
     2461 aggctgaacc atttcaaaaa aaactataga gaggtttcgt atgttactcc aagagaagac
     2521 gacatttgag tttagcaccg atgatgcgcg catggccgaa ctgatcgggc tggcgcagca
     2581 gattgaagac gtgaaggcgc tctccgcgct tgccgagtgg gaccagaata ccgagatgcc
     2641 ggaaggcgcg ggtgaggtac gtggcaacca gatggcaacc atccagggcg tcctgcacga
     2701 gttcaggacc aaccctcgcc tggggacact actcgacgaa ctggaaacgc gaattgggca
     2761 ggccgagtac accgacgccg atgccggatt gctgcgcgaa atgcgacgca tgtacgacca
     2821 ggcgaccagg ctgccacgca ctctggtgga agagctggcg cgcgtgagcg cgggcagctt
     2881 cgaggcatgg cgacgcgcgc gcgaacgcaa tgacttcgcc agcttcgccc cctggctgaa
     2941 acgcatggtc gccttgcagc gcgaggtagc ggatcgcttc ggctacgaag agacgcgcta
     3001 cgacgccctg ctcgacctgt acgagccagg catgacggtc agcaagctcg ataagctgtt
     3061 cgagccggtg cgcgcagtca gcgcaacgct gctgcggcgc atcgaagcta gcggtcacag
     3121 cgtagacgac agctgcttgc agggcgactt tgatccggat aaacagcttg aattgggccg
     3181 caagatactc aaaggcatgg gttacgattt ctcgcgcggc gcgctcgccg tctcgcccca
     3241 tcccttcacg accggcctgg gcagcccctt cgatgtgcgc gtgacggttc tcccgcaccg
     3301 cgacctcctt caagccagca tcatggcggc tatccacgag ggcggccacg ccctctacga
     3361 gcagggcagc gccgaaacgc tggcgcgcac ccccatcgcc ggaggggcct cgatgggcgt
     3421 tcacgagtcg cagtcgcggc tatgggagaa cgccatcggt cgctccaccg ccttctggca
     3481 ggggcaattc gccgccatgc gtgagacctt cccacgtccc tacgccgagg ttgacccggc
     3541 tgccttcgcg cgcgccctca acaaagtaca gccgagtctg atccgcatcg aggccgacga
     3601 ggtgacgtac aacctgcaca tcatcgtgcg ctacgagctg gaaaaggcca tggtcaacgg
     3661 cgacgtggac atcgagagcc tgccccgtct ctggaatgcg aagtatcgcg aatacctggg
     3721 aatcgagcca caacgcgatt ctgagggcgt cttgcaggac attcactgga caagcggctt
     3781 cggctacttc ccgtcctaca cgctcggcaa cctctacagc gcgcaaatct acgccaccct
     3841 gcgccaggtc tatcccgact tcgacgaacg cctgtcgcac ggcgataccg ccttcatcct
     3901 gaactggctg cgcgaacaca tgtacaccgc cggcgccacc tacctgcccg ataccctcat
     3961 gaaacaggtc acaggcgaag cgcccacgcc ggcctacttc gtgcgctatc tgacaggcaa
     4021 atttgagcgg atctacggac tgtagtttgc agggcaaaac aaaagcaggg caagtcttac
     4081 aattgccctg cttttgtttt gccctgcaaa caaattagcc actgagagcg tttcgtaaat
     4141 aggacaaatg accctttttt ctcctattgg cctggcagtg tttttctgac gaaaagatac
     4201 gacgtaccat ataaccagga aaatttacta cttttattcg aagtgacaac aaaagaagaa
     4261 acggacggag aagccaattc atttacaatt gatctctttc tttttaattg acagtaccat
     4321 ataataacac ttatatggta ctgttcagac cttacatcaa gcactggtat atttgatttt
     4381 gagagggttt gtacgtttag accactagat cgtttcttgt atgcttcgac gtaggtttgt
     4441 tagcgctgac aactctctga gactattcac acgaattcct
//
LOCUS       DASZAK010001085         6027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_132821, whole genome shotgun sequence.
ACCESSION   DASZAK010001085 DASZAK010000000
VERSION     DASZAK010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6027
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132821"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VGT44_19155"
     CDS             complement(<1..534)
                     /locus_tag="VGT44_19155"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopC"
                     /protein_id="HEV2582986.1"
                     /translation="MRKANHIHILAIFISIGLFFAITGTAAAQTTTPDTPALPAHASV
                     TRAIPAIGSTISQAPTSVTIFTAENINPDPKVSNLFVYGPVGAASDVLISQGNAQVSI
                     TNPEEMSINITPNLQHVNGVYVVHWITKSALDGDPDEGAFIFTVNTGAAATATPAATT
                     TSSTGQITTPPTTNTSGG"
     gene            complement(680..1654)
                     /locus_tag="VGT44_19160"
     CDS             complement(680..1654)
                     /locus_tag="VGT44_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582987.1"
                     /translation="MTDRIEPRKAWDDGLEALVYENPDAFIHWLLGDGLFKEKLPNTL
                     KTWKLEVDALLKVGFEGEDMLLHIEFQTYHDAIMPERLLRYNVLLRGEYQLPVSSFVI
                     YLLKNGPVAEPPLVWKVPPRGHEMLHFDYDSIELSDMTPEDLLEIGQTGLLPLLPLTR
                     GGARREVTNLMLERLRTADKQELISIGGTLAAYMMGRENQDDLIWLQRRLREMHDILR
                     ESPYYQEILEEGREEGLEKGLEKGLEKGREEGRELERLEHMQELRQAIINIVLGRYPK
                     LLRFARKQVAVVDDLATLRGLIVELSLAESPEQAKRSLEKVDEDEDED"
     gene            1767..2522
                     /gene="rnc"
                     /locus_tag="VGT44_19165"
     CDS             1767..2522
                     /gene="rnc"
                     /locus_tag="VGT44_19165"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV2582988.1"
                     /translation="MEYEEKLVELEKLLQVGFRDRVLLLRAITHHSCCPDTAVYDSYD
                     TLEFLGDSILGAHVVEHIYRSFPDASEGEMTALKSEAVSRRVLARIGQELGLFPYIRV
                     DIANLRTFNERSRDSLCADVIEAIIGAILIDQGYEAARDFVARVILPVVVPLSQQTLD
                     TNPKGQLQKLVLQRTGSLPRYRVMEQSGRNNDRFFTVGVFEHEFLLGVGKASSIKEAG
                     RLAAREALEILREQEAEVDQEQEEYLEQEERQV"
     gene            2541..4160
                     /locus_tag="VGT44_19170"
     CDS             2541..4160
                     /locus_tag="VGT44_19170"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEV2582989.1"
                     /translation="MPRTGILDKQQERAGLSCAPGVSDLFNWYEAGMPAMERKRRGHY
                     STPLPLVDQILDACGYTPEADLRRVRVLDPACGSGNFLAMAARRLIASTVGMGCSRRE
                     QAQLLERNIWGFDPDPVSCFLAKMQLSSQFAGNWHIHQADSLALNWAPCVDLFLANPP
                     YLAAKNADLSGYRSALQRGQADSYLLFLELAMRVVRPGGWIGLVLPDPLLARLNAARE
                     RAQLLRQMTLQHLWHLSDVFAAEVGAVVLVAQNVPPATTHQVAWERGHWTASLPLLVA
                     KAQKAETRRVAQARLLSQPAAELRYLLSGEHGELLDRMRAVLEDTGERRLAPLGDFVT
                     ISRGEELGRESQFIRPNTGMLDNTHRRGYAVLRGGIDMRPYTQPQAEWRIDHEAVTKP
                     MQRYLAPKLLVVKSTDRLQATIDTRGHVALQTLYLLHVRDCQEVQEPGDRLYFFLALL
                     NSRLLREYVSVLHTAYKWVQPQIEQRVLASLPVPLIERAARQKIIDQAKQLERACSEG
                     GGVVEWSEEIKALYETLERLIRALYLSGCGV"
     gene            4188..4619
                     /locus_tag="VGT44_19175"
     CDS             4188..4619
                     /locus_tag="VGT44_19175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEV2582990.1"
                     /translation="MAEIHSTRPIRVLVAKPGLDGHDRGAKVIARALRDAGMEVIYTG
                     IRQTPEMIVEAAIQEDVDAILMSILSGAHMAIFPKVMELLQQNGVDDVLVAAGGILPD
                     EDIPAIKAMGIKGCFGPGTSTEEIIEFVRANVQPDRLTADV"
     gene            4627..5589
                     /gene="meaB"
                     /locus_tag="VGT44_19180"
     CDS             4627..5589
                     /gene="meaB"
                     /locus_tag="VGT44_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEV2582991.1"
                     /translation="MQNIVERLLSGDRRALARMVTLIENDAPVARQYLAELHMHAGNA
                     HIVGITGAPGAGKSTLVTHMTRELRRRERKVGIVAVDPSSPFSGGAILGDRIRMMELA
                     GDPNVFIRSMASRGNLGGLSASTRDVVRAMDAAGYDPIIIETVGTGQAEVEVMRAAQT
                     VLVVAAPGMGDEVQAIKAGILEIADIFVVSKADKPGAEQTVAELAMLLSLDPMRRQHD
                     KSQPYWRIPVLKTSAIKDQGLVELIDAVEQHRAFLVKSGTLSQRAQRQVRSEVQALLI
                     HAMMRALQQEVSEEEWRELIEDINARERDPYSVARALQEKIGLK"
     gene            complement(5596..>6027)
                     /locus_tag="VGT44_19185"
     CDS             complement(5596..>6027)
                     /locus_tag="VGT44_19185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582992.1"
                     /translation="AAADHYMESLAIAALDQQVRLQLINYVAMAELRLDENRLDEART
                     YCDNADEICSEIEGDHHDCGMMYLVRGKVEQRAAHQDEDDCLLKDALVYFEQARQHLT
                     NSQARTTLSQLYGHMAEVYEELGQSDEALVHWKQAFAARSA"
BASE COUNT         1199 a         1563 c         1901 g         1364 t
ORIGIN      
        1 tccaccgctc gtattcgtgg tgggtggtgt ggtgatctgc ccggttgatg aggtggtggt
       61 agcggcgggg gtagcggtgg ccgccgcgcc ggtattgacg gtgaagataa aggcgccctc
      121 gtccgggtcg ccgtcaaggg cggatttggt gatccagtgg acgacgtaca caccgttcac
      181 gtgctggagg tttggtgtga tgttgattga catttcttcc gggttcgtaa tcgaaacctg
      241 ggcgttgccc tggctgataa gaacatcgct cgctgcgccg acaggcccat acacgaagag
      301 gttgcttacc tttggatcgg ggttgatatt ctcggcggtg aagatggtga cgcttgtggg
      361 ggcctgcgag atggtcgagc cgatggcggg tatagctcgc gtcacgctgg cgtgtgctgg
      421 aagagcgggt gtgtcgggtg tggttgtctg ggcggcggca gttcccgtta tggcgaagaa
      481 caggccgata cttatgaaga tggctaaaat atggatgtga tttgctttac gcatactatg
      541 tgttcgcttt gcatcataaa ggctcactgc attcaggcga ttgaatcatt tgcagcgcaa
      601 gcacagtata ctccgacgct caagaggcga ctatcggtac aaatccctat tctgtcacct
      661 gatttttgag tctcgcgatt cagtcctcat cttcgtcttc atcgactttc tctagagatc
      721 gtttggcttg ctccggcgac tcagcgaggc ttagctcgac gattagcccg cgcagggtgg
      781 cgagatcatc gacgactgcg acctgtttgc gcgcaaaacg cagcaattta ggatagcgcc
      841 ccagcacgat attgataatc gcctggcgca gttcctgcat atgctcgagt cgttccaatt
      901 cgcggccttc ctcccggcct ttttctaatc ctttttctaa tcctttttct aatccttctt
      961 ctcggccttc ctccagtatt tcctgataat agggagattc tctaagaatg tcgtgcatct
     1021 cgcgcagcct cctttgtagc catatgagat catcctggtt ctctcgaccc atcatatacg
     1081 ctgctaacgt gcctccaatt gagatcaact cttgtttgtc agccgttcgc agccgctcaa
     1141 gcatcagatt ggtgacttcg cgccttgccc cgcctcgcgt tagtggcaag aggggcaaca
     1201 gtcccgtttg tccgatttcc agcaggtctt ctggtgtcat gtcgctcagt tcaatgctat
     1261 cataatcaaa atgcagcatc tcgtgtccac gcggaggaac cttccagact agcggtggtt
     1321 cggccaccgg gccattcttg agtaagtaga tcacgaacga cgagactggc aactgatatt
     1381 cgcctcgcaa taagacatta taccgcaaga gccgctctgg catgatcgca tcgtgatagg
     1441 tctgaaactc gatgtgtagc agcatgtcct caccctcaaa tccgaccttg agtaatgcat
     1501 ccacttctag cttccaggtt ttgagcgtgt tcgggagctt ttctttgaac agtccatcac
     1561 cgagtaacca gtgaataaag gcatctggat tttcatatac cagggcctcc aagccgtcgt
     1621 cccacgcttt gcgtggttca atcctatccg tcatacgaga gatgcgctcc tacttaacca
     1681 ttcttaacca gagtgtaaca tagcggaaat ggcttgtcga gagctaattc tcgacgtcat
     1741 atgttttgtt acggtataat atgcgcatgg aatacgagga aaagctggtc gaattagaga
     1801 agctgctgca agtcgggttt cgtgatcgcg tgttgttgct gcgcgcgatc acgcatcatt
     1861 cgtgctgccc ggatacggcg gtctatgatt cctacgatac gctggagttt ctgggggact
     1921 cgatcctggg ggcgcatgtc gttgagcata tttatcgcag cttccccgat gcctcggagg
     1981 gcgagatgac ggccctgaag tccgaggcgg tgagccggcg cgtgctggcc aggatcggac
     2041 aggaactggg gctgttcccc tatattcgcg tggatattgc gaatttgcgc accttcaacg
     2101 agcgttcgcg cgattcgctg tgcgctgacg tgatcgaggc gatcattggc gcgatcctga
     2161 tcgaccaggg atatgaggcg gcgcgtgatt ttgtagcgcg cgtgattctg ccagtggtgg
     2221 tgcctttaag ccagcaaacg ctcgatacca atcccaaggg acagctacag aagctggtct
     2281 tgcagcgcac aggcagtctg ccgcgctacc gcgtcatgga gcagagcggg cgcaataatg
     2341 atcgtttctt cactgttggc gtgtttgagc atgagttttt gctgggcgtg ggaaaggcat
     2401 ccagtatcaa ggaagcgggt cggctggccg cgcgcgaggc gttggaaatc ttacgcgagc
     2461 aagaggcgga ggtcgatcag gagcaggaag aataccttga acaggaagag cgccaggtat
     2521 aaggccgttt gggaggatgt atgccgcgca cagggattct cgataagcag caagaacggg
     2581 ctggactatc ttgcgcaccg ggcgtatctg acctgttcaa ctggtacgag gccggtatgc
     2641 ctgcgatgga acgcaagcga cgcggtcatt actcaactcc cctgcccctg gtggatcaga
     2701 tactggatgc ctgcggctac actcctgaag cagacctgag gcgtgtgcgt gtgctggacc
     2761 ctgcttgtgg cagcggcaat ttcctggcga tggcggcgcg gcgtctgatt gcctctaccg
     2821 taggtatggg atgttcgcgg cgagaacagg cgcagctgtt ggagcgaaat atctgggggt
     2881 ttgatcctga tcccgtctcg tgcttccttg cgaagatgca actctcctcg caattcgcgg
     2941 gcaactggca tattcaccag gcggatagcc tggcgctcaa ttgggcgccg tgtgtcgatt
     3001 tatttctggc gaatccgccc tacctcgcgg cgaaaaatgc cgatttgagc gggtatcgct
     3061 cggcgctgca gcgcgggcag gctgatagct acctgctgtt tcttgaactg gcaatgcggg
     3121 tggtgcgacc gggaggatgg atcgggctgg tgctgcccga cccgctgctg gcgaggctga
     3181 atgccgccag ggagcgggcg caattgctgc ggcagatgac gctgcaacat ctctggcacc
     3241 tgtcagatgt cttcgcggcg gaggtcggcg cggttgtgct ggtggcgcaa aatgtgccac
     3301 cggcgacgac gcaccaggtc gcgtgggaac gggggcactg gacggcttca ctgccgctat
     3361 tagtggcgaa agctcaaaag gcagagacgc gcagggtggc gcaggcgagg ctattgagcc
     3421 agccggcagc cgagttgcgc tatctgttga gtggcgagca tggcgagttg ctcgatcgga
     3481 tgcgcgctgt tctggaagat acaggtgagc gccggcttgc gccgctgggc gattttgtga
     3541 cgatttcgcg tggcgaagaa ttgggccgcg agagccagtt tatcaggccg aacacaggta
     3601 tgctggataa cacacaccga cgaggctatg ctgttttgcg cggtggcatc gacatgcgac
     3661 cctacacgca accgcaagcg gaatggcgca ttgaccacga agcggtgacg aagcccatgc
     3721 agcgctacct tgcgcccaag ctgctcgtgg tgaagagcac cgaccgtttg caggcgacga
     3781 ttgatacacg cggacatgtg gcgctgcaaa cgctgtattt gctgcacgtc cgggactgtc
     3841 aagaggtgca ggagccaggt gatcggctgt atttcttcct ggcgctgctc aattcgcggc
     3901 tgctgcgcga gtatgtctct gtcctgcata cggcctataa atgggtgcag ccgcagatcg
     3961 agcagcgtgt gctggcctcg ctgcccgtgc cgctcatcga acgcgccgcg cgccagaaaa
     4021 taatcgatca agcgaagcaa ttggaacgcg cttgcagcga gggagggggt gttgtagaat
     4081 ggagcgagga gatcaaggcg ctgtacgaaa cgctggagcg attgatacgc gcgttgtatt
     4141 tgtctggttg cggtgtttaa cgatttgtag ggacagcgag gtgggatatg gctgaaatcc
     4201 actctacacg cccgatccgt gttctggtag ccaagccggg gctggatggg catgatcgcg
     4261 gggcaaaggt gattgcgcgt gcgctgcgcg atgccggtat ggaagtgatc tatacgggca
     4321 ttcgccagac acccgaaatg atcgttgagg cggctatcca ggaggacgtg gatgcaatct
     4381 tgatgagcat tctctccggc gcgcacatgg cgatttttcc aaaggtgatg gagctgttgc
     4441 agcagaatgg tgtcgatgat gtgctggtgg cggctggggg cattctgccg gacgaggata
     4501 ttccagcgat caaggctatg ggcatcaagg gctgttttgg ccctggcacc tccactgaag
     4561 aaatcattga atttgtgcgt gcgaatgtgc agcccgaccg gctgacggca gatgtttagg
     4621 tgcagtgtgc agaacattgt cgaacgcctg ttgagcggag accgccgcgc gctcgcccgc
     4681 atggtgacgc tgatagagaa tgacgccccc gtcgctcgcc agtacctggc tgaattgcac
     4741 atgcacgccg ggaatgcgca tattgtcggc atcaccggcg cgcccggcgc gggcaagagt
     4801 acgctggtga cgcatatgac gcgcgagctg cggcggcgcg agcgcaaggt cggcatcgtg
     4861 gctgttgatc cgagcagccc tttttctggc ggggcgatcc tgggagatcg tattcgtatg
     4921 atggaactgg ccggagaccc caatgtcttc attcgcagca tggcgagtcg cggcaatcta
     4981 ggcggtcttt ccgcttcgac gcgcgacgtg gtgcgcgcca tggacgcggc ggggtatgac
     5041 ccgatcatca tcgagacggt cggcaccggc caggcggagg tcgaagtgat gcgggccgcg
     5101 caaacagtgc tggtggttgc cgcgccgggc atgggcgacg aggtgcaggc catcaaggct
     5161 ggtatcctgg agatagcgga tatattcgtt gtcagcaaag ccgataagcc aggcgcggag
     5221 cagaccgtcg cggaactggc gatgctgctc agcctcgacc ccatgcggcg gcagcacgac
     5281 aaatcgcaac cctactggcg tattccggtt ctgaaaacgt cggcgattaa ggatcagggt
     5341 ctggtagaat tgatcgacgc ggtcgaacaa catcgcgcgt ttctggtgaa gagcgggaca
     5401 ctttcgcagc gcgcacagcg ccaggtgcgc agcgaagtgc aggcgctgct gatccatgcg
     5461 atgatgcgcg cgctgcagca agaggtgtcc gaagaagagt ggcgcgaatt aattgaggat
     5521 atcaatgcac gcgaacgcga tccgtacagt gttgcgcggg cgttgcagga aaagatcggg
     5581 ctgaaatagc cgctgctagg ccgacctggc ggcgaaggct tgtttccaat gaaccagggc
     5641 ttcgtctgat tgtcccaact cctcgtagac ttcggccatg tgaccgtata actggctcaa
     5701 tgttgtgcgc gcctgggagt tggtgagatg ttgcctggcc tgctcgaaat agacgagcgc
     5761 gtccttcaag aggcagtcgt cctcgtcctg gtgggcggcg cgctgctcaa ccttcccacg
     5821 caccaggtac atcatgccac agtcgtggtg gtcgccctcg atctcactgc aaatctcatc
     5881 agcgttatcg cagtaagtcc tggcctcatc caggcggttt tcgtcgaggc gtagctcggc
     5941 cattgcgaca taattaatca gttgcagacg gacctgttga tctagagcgg cgatcgcgag
     6001 cgattccata taatggtcgg cggcggc
//
LOCUS       DASZAK010001086         3584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_132822, whole genome shotgun sequence.
ACCESSION   DASZAK010001086 DASZAK010000000
VERSION     DASZAK010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3584
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132822"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VGT44_19190"
     CDS             <1..386
                     /locus_tag="VGT44_19190"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2582993.1"
                     /translation="VNFAPLGGSAPLRAIIAALRKNEVVLITGDRAIVGESVERDFFG
                     ARTRLPSGPISLALRTGAMVVGAVGWYKRRGRIGGHFTPISLAMPEEERVNEERLHTK
                     VVEMMEREIRCHPEQWLVFAPVWED"
     gene            complement(383..1120)
                     /locus_tag="VGT44_19195"
     CDS             complement(383..1120)
                     /locus_tag="VGT44_19195"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEV2582994.1"
                     /translation="MNNIHNTSGYSRADTHMHTNLGDGWASPAQVIKIALMRDLQLIA
                     ITDHDHVEGAKRVADLLARQELPLQMITGVEVSTRQGHLLGLFVQKAPRSLRSVEESI
                     EAIKEQGGLVIVPHPLGRLVPSLSRDKIDELLNKGFAIDGIEIYNPSPANASMRLAVR
                     NANQQWRLAETGGSDAHFWQHIGSAYTLFPGSTPDDLRRSILERTARPGGQEIPPARL
                     SPAAYVAQCAWSWFVDPPRRIARSLRS"
     gene            complement(1144..2310)
                     /locus_tag="VGT44_19200"
     CDS             complement(1144..2310)
                     /locus_tag="VGT44_19200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV2582995.1"
                     /translation="MKIGLVSPYDWSYPGGVRDHVSHLAEQFIAMGHDVRILAPASGI
                     KAKIHEKNVVKMGWTTPIPINGSIARILIDPSLAIRVRRVLQREHFDIIHVHEPLVPG
                     LSQQVLRFSHALNVGTFHAASYSHMYSTSQLAYASTSPLLRPLFRKLAGCIAVSTAAR
                     QFIARYFPADYRIIPNGISLDRFHPDILPLAKYMDGKLNILFVGRFEKRKGAKYLLRS
                     IPYIRERHPNTRFIFVGEGRLRPGFQRYVEKQGWHNVVFTGYVSDEDLPRYFASAKVF
                     CAPAIGGESLGIVLLEAMAAGKPVVASDIRGYATVIDNGIDGLLTPPRQPEELANAIN
                     TLLENEALCQRFIQNGLLKARDYAWPYVAKSIMNYYMELLEGRNRQTRRFASNA"
     gene            complement(2340..>3584)
                     /locus_tag="VGT44_19205"
     CDS             complement(2340..>3584)
                     /locus_tag="VGT44_19205"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HEV2582996.1"
                     /translation="DAVLWTDLPPGTSPLTSEQVPFSHPLWVLYSSGTTGLPKPIVQS
                     QGGIIIEHLKALHIGKDLTTDDRFFWFTTTGWMMWNLLLGGLLLGVPVLLYDGSPGYP
                     DMNVLWKFAEQSGMTFFGTSAGFILACMKAGVTPGKSFDLSKLRGLGSTGSPLPPEGF
                     QWIYEQIKSDLWLASISGGTDVCSAFLGGCVLLPVYAGELQTRLLGAKIEAFDENGQP
                     LVDEVGELVITEPMPSMPIFFWNDPDGKRYRESYFDMYPGVWRHGDWIKLTHRGSAII
                     YGRSDSTITRKGIRMGSSEIYRVVEDLPEVLDSLIIGIERPGGDYFLPLFVVLRPGVE
                     LDDALKARIRNRLRTSLSPHHVPDDIFVIPEVPRTLSGKKLEVPVKKIFMGVPIEKAI
                     SVDAMSNPQVLQYFVDFAHKQQ"
BASE COUNT          710 a          950 c         1181 g          743 t
ORIGIN      
        1 gggtcaactt tgcgccgtta ggcggaagcg cacccctgcg cgccataatt gcggcgctgc
       61 gcaagaatga agtggtctta atcacggggg atcgcgccat cgtgggtgaa agcgtagagc
      121 gcgatttttt cggcgcgcgg acgcggctgc cctctggccc gatctcgctg gcgctgcgca
      181 cgggcgctat ggtggtgggc gcagtcggtt ggtataaaag gcgggggcgc attggaggcc
      241 atttcacgcc gatttcactg gcaatgccgg aagaggaacg tgtcaacgaa gagcgtttgc
      301 acacgaaggt cgttgagatg atggagcgag agatccgatg ccacccggag caatggctcg
      361 tgtttgcgcc ggtatgggag gattaacttc tcagcgatcg cgcaatcctg cgcgggggat
      421 cgacgaacca gctccaggcg cactgcgcga cgtaggcggc aggcgagagg cgcgcgggcg
      481 ggatttcctg gccgccgggg cgcgcggtgc gttcgaggat ggagcggcgc aggtcgtcgg
      541 gcgtgctgcc ggggaagagc gtataggccg agccgatgtg ctgccagaaa tgagcgtcgc
      601 tgccgcccgt ttcggccagg cgccactgct ggttggcgtt gcgcacagcc aggcgcatag
      661 aggcgttggc gggcgacgga ttgtagattt cgatgccgtc gatggcaaag cccttgttga
      721 gcagttcgtc gattttgtcc cggctgaggc tgggaaccag ccgtcccagc gggtgaggca
      781 caatcaccag cccgccctgc tctttaatag cctcgatgct ctcttcgacg gaacgcaaag
      841 acctcggcgc tttctggacg aagaggccga gcaggtgtcc ctggcgcgtc gagacttcga
      901 cgccggtgat catttgcagg ggcagttcct gccgcgccag tagatcggct acgcgctttg
      961 cgccctcgac atggtcgtgg tcggtaatgg cgataagttg caggtctcgc atcagcgcga
     1021 ttttgatgac ctgcgccggg ctggcccaac cgtcgccgag gttggtgtgc atgtgggtat
     1081 cggcgcggct gtagccgctc gtgttatgta tgttattcat tactttcttc ttctttcgtg
     1141 caattatgca ttcgatgcaa agcgcctcgt ttgccggttg cggccttcga ggagttccat
     1201 atagtagttc atgatactct tcgcgacgta gggccaggca tagtcacgcg ctttgagcag
     1261 gccgttctgg ataaaacgct ggcacagcgc ctcgttttcg agcagcgtat tgatggcgtt
     1321 ggccagctct tcgggctggc gcggcggcgt gagcaggcca tcgatgccgt tatcgatcac
     1381 agtggcatat ccgcgaatat ccgaggcgac gacgggtttt ccggcggcca tggcttcgag
     1441 cagcacgatg cccaggctct cgccaccgat ggccggggcg caaaagacct tcgcgctggc
     1501 gaagtagcgc ggcaaatcct catcggagac gtagccggtg aagacgacgt tgtgccagcc
     1561 ctgcttttca acgtagcgct ggaagccagg acgcagacgg ccctccccga cgaaaatgaa
     1621 gcgcgtgtta gggtgacgtt cgcgaatata cgggatagag cgcaacaggt atttggcccc
     1681 tttgcgtttc tcgaagcgtc ccacgaacag gatattcaat ttgccatcca tatatttcgc
     1741 aagcggcagg atgtctggat gaaaacggtc caggctgatg ccattcggga tgatgcgata
     1801 atcggctggg aagtagcgcg cgatgaactg ccgcgcggcg gtcgagacgg cgatgcagcc
     1861 cgccagcttg cgaaagagtg ggcgcagcag cggagacgtc gaggcatagg cgagctgtga
     1921 agtcgagtac atatgcgaat acgacgcggc gtgaaaggtt cccacgttga gcgcgtggga
     1981 gaaacgcagc acctgctgtg acaggccagg cacgagcggc tcatgcacgt gaatgatgtc
     2041 gaaatgctcg cgctgcaaga ccctgcgcac acgaatggcc agcgatgggt cgatgaggat
     2101 gcgggcgatc gatccgttga tggggatggg ggtggtccag cccatcttca caacattttt
     2161 ttcgtggatc ttcgctttga tgccagaggc cggggcaagg atgcgcacgt catgtcccat
     2221 ggcaataaat tgttcggcaa ggtgtgaaac atgatcgcga actcccccag gataactcca
     2281 atcatacggt gacacgaggc caattttcat gcgcgacttc cttatccgtt tctgtcggtt
     2341 tattgttgtt tgtgggcgaa atcaacgaaa tattgcaaca cctgtggatt gctcatcgca
     2401 tcgacgctga tggctttttc gattggcacg cccatgaaga tttttttgac cggcacttcc
     2461 agcttcttgc cgctcaaagt gcgaggcact tcaggtatga cgaatatatc atcgggaaca
     2521 tggtgtggtg agagactggt gcgtagtcga ttgcggattc tggctttgag cgcatcatcc
     2581 agttcgacgc cgggacgcag cacgacgaag agtggcagga agtaatcgcc gccggggcgc
     2641 tcgatgccaa ttatcaggct atccaggact tctggaaggt cttcgacgac acggtagatt
     2701 tcgctgctgc ccatgcgaat acctttgcga gtgatggtgg agtcggagcg accgtagatg
     2761 atggcgctgc ccctgtgggt gagcttgatc cagtcgccat gccgccagac gccgggatac
     2821 atgtcgaagt agctctcgcg gtagcgtttg ccatctggat cgttccagaa aaagatgggc
     2881 atggacggca tgggttcagt gatgacgagt tcgcccacct cgtcgacgag aggctggccg
     2941 ttttcgtcga aggcttcaat tttcgcgccg agcaagcgag tttgcagctc gccggcgtag
     3001 acgggcagca gcacgcagcc gcccaggaag gccgaacaga catcggtgcc gccgctgata
     3061 gatgccagcc agaggtcgga cttgatctgc tcgtaaatcc actggaagcc ttcgggcggc
     3121 agcggcgagc cggttgagcc aagcccgcgc agtttgctca ggtcgaagct tttgccgggc
     3181 gtcacgccgg ccttcataca ggccaggatg aagcccgcgc tggtgccgaa aaaggtcatg
     3241 ccggattgtt cggcgaattt ccagaggaca ttcatatcgg gatagccggg gctgccgtcg
     3301 tagagcagca cgggaacgcc gagcagcagt ccgccgagca gcaggttcca catcatccag
     3361 ccggtggtgg taaaccagaa gaaacggtca tcggtggtca gatccttgcc aatgtgcagg
     3421 gctttgagat gctcgataat gatacctccc tggctctgca cgatgggctt tggcaggcca
     3481 gtcgtcccgg aggagtagag tacccagagt gggtgtgaga acggcacctg ctcggaggtc
     3541 aggggcgatg taccgggtgg caggtctgtc cagaggacgg catc
//
LOCUS       DASZAK010001087         2656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_277519, whole genome shotgun sequence.
ACCESSION   DASZAK010001087 DASZAK010000000
VERSION     DASZAK010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277519"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..779)
                     /gene="pgl"
                     /locus_tag="VGT44_19210"
     CDS             complement(42..779)
                     /gene="pgl"
                     /locus_tag="VGT44_19210"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEV2582997.1"
                     /translation="MHIAVFPDLDTISQHAAQYVTRVANEAIVTRGRFTIALSGGNTP
                     RKLYHLLGTEPYRGQIDWNFVDIFWSDERCVPPDDPESNYRMAREELLDHIQTPASQV
                     HRMPADQPDRDAASLAYTREMQRVFSTDGIPTFDLIQLGMGPEGHTASLFPHQPSLHE
                     TARLVMPVSVPKPPPDRLTFTPPLLNVARNILFLATGADKADVMQMVAEGPHNPDEYP
                     AQVVNPAHGEVVWMLDTAIAKNLTVGR"
     gene            complement(801..2054)
                     /locus_tag="VGT44_19215"
     CDS             complement(801..2054)
                     /locus_tag="VGT44_19215"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HEV2582998.1"
                     /translation="MTNNTANGPGIRLPWAGKNVRIDEVEQRLASLWSMSHDNLRTGA
                     NVNVRTSVLNLIICTPDIETASRANKVLRELASSHLARVTILILDRSAENSAMTAWVT
                     LRCFSMISDLMRHCFEQTTMVASGSAIRAASNMLQPILKPELPVYLWWLGDPPDSSDP
                     TFQNLVDLSSRIIFDSTTFFQPEEDIRTLAELCDAAPRSALSDLNWGRITPWRQLISQ
                     FFDVAEYRPYLAGIHSIEIEHAVAPLAQQVQTVEGDVSPNPSRALLLSSWLKDRLGWS
                     MAEDTSKNHQHTATGYYHWEMVRQAPSRGTRQLGSTRSRAGANATITIRPKPDTEVRP
                     GSLSLVRLTSTLDNRQAVFTIGRENEPDHVYTSVEIGSDVRQRRVVSIPISQREFELL
                     HEELEITSHDFIFEQTIKDIAGLFD"
     gene            complement(2170..>2656)
                     /locus_tag="VGT44_19220"
     CDS             complement(2170..>2656)
                     /locus_tag="VGT44_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV2582999.1"
                     /translation="GVPFYLRTGKHLPKRVSEIAIQFKQAPLVLFKRSEAYGQVEPNV
                     LTLRIQPDEGISLKFGAKVPGTEMQIRSVNMDFIYGSSFFREQPEAYERLLLDCMLGD
                     STLFTRRDEVEAAWGFLQGILDEWRSEPADTIQTYESGSWGPQLADEFIWRDGRRWRR
                     P"
BASE COUNT          524 a          705 c          844 g          583 t
ORIGIN      
        1 agggcgcaat gaattgcgcc cctacatcgg ttaacatcct atcacctgcc tacggttaga
       61 ttttttgcaa tcgccgtatc cagcatccac accacctcgc catgcgccgg gttcacgacc
      121 tgcgccgggt attcatccgg gttgtgaggt ccttccgcga ccatctgcat gacatccgct
      181 ttgtccgcgc ctgtcgcaag gaagaggatg ttgcgcgcga cgtttagcag ggggggagtg
      241 aaggtcaggc ggtcgggtgg gggcttggga acggagacag gcatcacgag gcgcgcggtt
      301 tcatgcagcg agggttggtg tgggaagagc gatgcagtat gcccctccgg tcccatgcca
      361 agctggatca ggtcgaaagt cgggatgcca tcggtactga agacacgctg catctcccgc
      421 gtgtaggcaa gcgaggcggc gtcgcgatcg ggctggtcgg ctggcatgcg atgtacttgt
      481 gaggcgggtg tctgtatgtg atccagcagt tcttcgcgcg ccatgcggta gttgctctca
      541 gggtcatctg gcggaacgca gcgttcatcg ctccagaaga tatcgacgaa gttccagtcg
      601 atctggccgc gatatggttc tgttccgagc aaatgataca gtttcctggg agtgttgccg
      661 ccggaaagcg cgatagtgaa acggcctcgt gtcacgatgg cctcgttcgc gacgcgcgtg
      721 acatattggg cggcgtgctg gctgatcgta tcgagatcag gaaaaacggc aatatgcatg
      781 ggcttctccc tacaggtcgg tcaatcgaat agtcccgcga tatctttgat ggtttgttcg
      841 aagataaaat cgtgcgaggt aatctccagt tcctcgtgta gcagttcaaa ttcacgctgg
      901 ctgatcggaa tgctgacgac gcgccgctgc cgcacgtccg agccgatttc aacgctggtg
      961 tagacgtgat cgggttcatt ctcccggcca atcgtgaaga cggcctgtcg gttgtcgagt
     1021 gtgctggtca gccgcacaag tgaaagcgag ccggggcgta cctcggtatc tggctttggg
     1081 cgaatggtaa tcgtagcgtt cgcgcccgcc ctgctgcgtg tcgagccgag ctggcgagtg
     1141 ccgcgcgatg gtgcttgtcg caccatttcc cagtgataat agccggtagc ggtgtgttga
     1201 tggtttttcg acgtgtcctc tgccatcgac cagccgaggc gatctttgag ccaggaagaa
     1261 agcagcagcg cgcgcgaagg gttgggcgag acatcgccct cgacggtttg cacctgctgc
     1321 gcgagcggcg cgacggcatg ttcaatttca atggaatgaa tgcccgccag gtaggggcga
     1381 tattccgcga catcgaagaa ctggctgatg agctgccgcc agggggtgat acgaccccag
     1441 ttgaggtcgc tcagcgcgct tctgggcgcg gcgtcgcaca gttcggccag ggtgcgaatg
     1501 tcctcttccg gctggaaaaa ggtggttgag tcgaaaatga tgcgactgct caaatcgacg
     1561 aggttctgga aggtcgggtc gctggagtcg ggtgggtcgc ccagccacca caggtatacc
     1621 ggtagttctg gtttgagaat cggctggagc atattgctgg cggcgcgaat ggcgctgccg
     1681 gaggcaacca tcgttgtctg ctcaaagcaa tggcgcatca agtcggaaat catcgagaag
     1741 cagcgcagcg tgacccaggc ggtcatagcg gagttttcgg cgctgcggtc gagtatgaga
     1801 atagtcacac gggccaggtg cgagcttgcg agttcgcgca gcaccttatt ggcgcgggag
     1861 gccgtttcga tatcgggggt acagatgatg agattcaaga cgctcgtgcg aacattgacg
     1921 ttcgcgccgg tgcgcaggtt gtcatgcgac atgctccaca atgaggctaa ccgctgttcg
     1981 acttcatcga tgcgtacatt ttttccggcc cagggcaaac gtatgcccgg tccattggcg
     2041 gtattattcg tcatgctcgt ttcccatttc tatgatggat acgccggttc tttactggtt
     2101 agacgcgata gaagacgccc tatcgagttg tcataatgac cagcacgtgc ctgaaatatg
     2161 acacagggct tatggccgac gccagcgtcg gccatcgcgc cagataaact catcggccag
     2221 ttgcggcccc caggaacccg attcgtaggt ctggattgtg tccgcaggct cactgcgcca
     2281 ttcatccagg atgccctgca ggaagcccca cgcggcctca acctcgtcgc ggcgcgtgaa
     2341 gagcgttgag tcgcccagca tacagtcgag caagagccgc tcgtaggctt cgggctgttc
     2401 gcggaaaaag gatgaaccgt agatgaaatc catattgacc gagcgtatct gcatttcagt
     2461 gccgggtact ttcgcgccga atttcaatga aatgccctcg tccggctgga tgcgcagcgt
     2521 cagcacgttc ggctcgacct ggccgtaggc ttcactgcgt ttgaagagaa cgagaggcgc
     2581 ctgtttgaac tgaatagcga tttcagagac gcgctttggc aggtgcttgc cggtgcgcag
     2641 gtaaaacggc acccct
//
LOCUS       DASZAK010001088         7412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_203626, whole genome shotgun sequence.
ACCESSION   DASZAK010001088 DASZAK010000000
VERSION     DASZAK010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203626"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VGT44_19225"
     CDS             complement(<1..528)
                     /locus_tag="VGT44_19225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583000.1"
                     /translation="MRQHDSFSSDFTTMLNALAQPGAFPFALPDSEVIEVMQTHASAV
                     LLAADRAYKLKKPKNFGFFDYSTPALRRHFCGQEVLLNRRLAPHVYLGVAPVLCYADG
                     RLRFGPASSPEKVAMPGAVVERGTVVDYAVVMARLPNEATLEYLVRAGTATPELLAAI
                     ARYVAKFHASTPTSEH"
     gene            complement(583..1746)
                     /locus_tag="VGT44_19230"
     CDS             complement(583..1746)
                     /locus_tag="VGT44_19230"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583001.1"
                     /translation="MNIPTRGELRYLMEPQGGPCITIMVSTHRIGMERQSDQLKLRHA
                     IRETEQRLHLEGVSAIDALLAPVKALLADEASWQQRGDGLVIYRSPRLFHLYCVPLHV
                     RDQVIISDHFSLKPLLPLLVGDGRFYVLALSQNAVRLLEGTRYSIQEIALPEAVPHSL
                     AEALKYDDIEENDVRHHSSSSGAVVGKGGRRAAVFHGQGVGIDDLKDQLLRYFQQVNR
                     GLHELLREEMVPMVLAGVDYLIPIYRQANTYPHLLDQEIVGNPDRLKAETLREQAWDI
                     VESILLKQEREAASRYRDLVGTWLASDAFEEALPAASYGRIESLFVALDQEQWGRFDP
                     ATNTLTLHTEAESGDIDLLDVAAIQTLLHGGKVYAVERREMPGDSLVAAVFRY"
     gene            complement(1866..2333)
                     /locus_tag="VGT44_19235"
     CDS             complement(1866..2333)
                     /locus_tag="VGT44_19235"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEV2583002.1"
                     /translation="MSMLQKKDPFEALVPLREAMNRLFEESFFWPGRLEFFTGRTFPV
                     DVIETEDKQQYTIKASLPGFKPEDIQITAEGDTLTIRVAKKGEEKEEKPTYVRHERYE
                     GEMIRSFTLPGRFEADQIAATYEHGVLSLTVPKAEAIKPKQIPITVKEAVGAR"
     gene            complement(2479..2946)
                     /locus_tag="VGT44_19240"
     CDS             complement(2479..2946)
                     /locus_tag="VGT44_19240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEV2583003.1"
                     /translation="MSILPLEVLVIGVGNMLRGDDGVGPAIIAALAARGLPGAQLMIC
                     QGDGLQLMETWKRADRVILIDAVTTGRKPGTVYRFDADQQALPADLSFSSTHAFGVVE
                     AIELARALDQLPSRLVIYAIEGQRFAIGTGLSPEVGRGLDDVVGRIVGEVDFM"
     gene            complement(2959..4248)
                     /locus_tag="VGT44_19245"
     CDS             complement(2959..4248)
                     /locus_tag="VGT44_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEV2583004.1"
                     /translation="MSDKTIKVDYLARVEGEGALYVKISGEQVTDVKFRIFEPPRFFE
                     AFLRGRAFGEAPDITARICGICPVAYQMSAVHAMEHAFGITVGGQLRALRRLLYCGEW
                     IESHTLHVYMLHAPDFLGYPDVIRMAQDHPDLVKRALQMKKTGNAIVSFLGGREIHPI
                     NVRVGGFYKVPAKREFGPLVEQLKWGRDAALETVRWVAQLPFPDFEQDYEFVALYHPN
                     EYPFNEGLLVSNKGLSIPVHEYEDHFVEEHVPYSNALHSVLKGRGAYFVGPMARYNLN
                     FDQLSPLAQEAARGAGLGQVCRNPFQSIVVRSVEILYAFDEALRIIEEYEQPDAPSVR
                     VRPVAATGCACTEAPRGILYHRYRLDEDGIIRDAKIIPPTSQNQKTIENDLWRFVEQH
                     KALPEEQLTWQCEQAIRNYDPCISCATHFLKLQVERE"
     gene            complement(4375..5151)
                     /locus_tag="VGT44_19250"
     CDS             complement(4375..5151)
                     /locus_tag="VGT44_19250"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583005.1"
                     /translation="MNREHKPKLAVWKFASCDGCQLSLLDCEDELLALTGEIEIANFA
                     EASRAVVKGPYDLSLVEGSITTPHDAERIQHIRRSSRFLVTIGACATAGGIQALRNFK
                     DVNEFISIVYATPQYIEVLNTSTPISDHVKVDFELRGCPINKRQLLEVISAYLNGRKP
                     NTPPHSVCMECKLRGTVCVMVAQHIPCLGPVTHAGCGAICPSYARGCYGCFGPKETPN
                     TASLSHQWQHLGAGRQEVIRVFRSFNAAAGPFRKASDQLS"
     gene            complement(5153..5992)
                     /locus_tag="VGT44_19255"
     CDS             complement(5153..5992)
                     /locus_tag="VGT44_19255"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEV2583006.1"
                     /translation="MSTPLSDPMLPKLYQVQRVRREIPGTFTMELEPQDGSAVPPFAS
                     GQFNMLYVFGVGEIPISISGDPDRPTPLVHTTRAVGTVSGAMQELKPGDTIGVRGPFG
                     SHWPIEQAAGKDVVIVAGGIGLAPLRSTVYQLIARRAQYGKIALLYGARTPSDMLFRH
                     ELERWRARLDLDVYITVDRGDASWRGSVGVVTQLIRRAVFDPHNTLALICGPEIMMRF
                     TTAELAKHGLVADHVYVSMERNMKCAVGLCGHCQYGPHFVCKDGPVFQYSQVQHLLVK
                     AEI"
     gene            complement(5989..6465)
                     /locus_tag="VGT44_19260"
     CDS             complement(5989..6465)
                     /locus_tag="VGT44_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEV2583007.1"
                     /translation="MKTLEPILANHPFFKGFGPRYMQLITGCASNVRFPAESYIFREG
                     EPASSFYIIRRGKVALEAAAAQRGSVVIQTIEEGEVLGWSWLFPPYRWHFDARTIEET
                     RAIALDGVCLREKAEADHDFGYELVKRVALIMMERLQATRLQALDIYDVPSVEASV"
     gene            complement(6462..>7412)
                     /locus_tag="VGT44_19265"
     CDS             complement(6462..>7412)
                     /locus_tag="VGT44_19265"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEV2583008.1"
                     /translation="KKRRDRALFGYNVGPHSWKQFLHPPTIRLWQAERKENGFQILPE
                     QQQPEKLAFLGVRSCELHAIAIQDRVFTNAHYRDPIYTTRRENVCIIAVNCGQAGETC
                     FCVSMQTGPQATGGFDLALTEVLDEERHFFVIEVGTDLGAAILQDVPYRAASEDDLRL
                     AGSAVANAAAHMGRSMETSDIKGLLYRNLDHPRWENVASRCLTCGNCTMVCPTCFCTT
                     VEDTTDLAGTQAERWRRWDSCFTTDFSYIAGGSIRASSKSRYRQWMTHKLASWIDQFG
                     TSGCVGCGRCITWCPVGIDITEEVRAIRESETARGREEQV"
BASE COUNT         1382 a         2270 c         2249 g         1511 t
ORIGIN      
        1 gtgttcgctg gtcggggtcg aggcatggaa ctttgcgaca tagcgtgcga ttgctgccag
       61 cagttcgggc gtcgccgttc ccgcgcgaac caggtattcc agcgttgctt cgttgggcag
      121 gcgcgccatc acgaccgcgt agtccaccac cgttcctctt tccaccacgg cgcctggcat
      181 cgcgactttc tcgggggaag aggcggggcc gaagcgaagc ctgccatcgg cgtagcacag
      241 cacgggcgcg acccccaggt agacatgcgg tgcgagccgc cggttcagca gcacctcctg
      301 accgcagaag tgacgtcgca aggctggggt cgagtagtcg aagaagccaa agtttttcgg
      361 ctttttcaat ttgtacgccc ggtcagccgc caggagtacc gccgaggcat gggtctgcat
      421 gacttcgatc acttcgctat cggggagagc gaaagggaat gctccaggct gtgcgagggc
      481 attgagcatt gttgtaaaat cgctggagaa ggagtcgtgc tgcctcatgg atttctcatc
      541 ggcctccctg gctttccctc ttaagcctgc acgggctgat cgctaatagc ggaagaccgc
      601 cgctactaac gaatcgcccg gcatctccct ccgctcgacc gcgtagactt tccccccatg
      661 cagcagcgtc tgaattgcgg cgacgtccag caggtctatg tcaccggatt ccgcttctgt
      721 atggagcgtg agggtgttcg tggccggatc aaagcggccc cattgctcct ggtcgagggc
      781 gacaaacagg ctctcgatgc gcccgtagga ggcggcaggc agcgcttcct cgaaagcatc
      841 ggaagcgagc caggtcccta ccaggtcccg gtagcgggag gcggcttcgc gctcttgttt
      901 caggaggata gattcgacga tgtcccaggc ctgctcgcgc agcgtttccg ccttcagcct
      961 gtcggggttg ccgacaatct cctggtcgag caggtgggga taggtattgg cctgtcgata
     1021 gatcgggatg agataatcta ctccggccag caccatcgga accatttcct cgcgcaggag
     1081 ttcatgcaat ccacgattga cctgctgaaa gtagcgcagg agctggtcct tgagatcatc
     1141 gatgcctacg ccctgcccgt ggaagaccgc tgcacgccgt ccccctttgc ccacgaccgc
     1201 gcccgatgaa gaactgtgat ggcgtacatc gttctcttcg atatcatcgt acttgagcgc
     1261 ctctgccagg ctgtggggaa ccgcttcggg cagcgcgatt tcctggatac tatagcgcgt
     1321 cccttcaagg agccggacag cgttttgaga gagagccagc acataaaagc gcccatcacc
     1381 aaccagcaga ggcaacagtg gcttgaggga gaagtgatcg ctaatgatga cctggtctct
     1441 tacgtggagt ggtacgcaat acaagtgaaa caggcgtggc gaacggtaga ttaccagccc
     1501 gtcgcctcgc tgttgccagg atgcttcatc tgccaacagc gccttgaccg gcgccagcaa
     1561 tgcatcgata gcgctaactc cttcaaggtg caagcgctgt tccgtttctc gtatggcatg
     1621 tctcagcttc aattgatctg attgcctctc cataccaatc cggtgcgtgg agaccatgat
     1681 cgtgatgcag ggaccgccct gcggctccat cagatatctg agttcgcctc ttgtcggaat
     1741 gttcattttg atcgactcct tccagctgcc ttcaaaggaa acaggaactg gaacacactg
     1801 gcaatcactg cctgctccag ttcctgctcc gcacaagggg cctcattgcc cctcacgctt
     1861 accgcttagc gcgcgcccac cgcttccttg accgtgatcg ggatctgctt tggctttatg
     1921 gcctcggcct tcggaacggt cagggacagc acaccgtgtt cgtaggtggc ggcgatctgg
     1981 tcggcctcaa agcgaccggg cagggtaaag gatcggatca tctcaccctc gtaacgctcg
     2041 tggcgcacat aggtgggctt ctcctccttc tcttcaccct tcttcgccac gcggatcgtc
     2101 agggtgtctc cctccgcggt gatctggatg tcctccggct tgaaaccggg caggctggct
     2161 ttgatcgtat actgctgctt atcttccgtc tcgatcacat cgaccgggaa ggtgcggcca
     2221 gtaaagaact ccaggcgccc aggccagaag aagctctcct caaacaggcg attcatggcc
     2281 tcccgaagcg gcaccaacgc ctcgaagggg tcttttttct gtagcattga catgctcttt
     2341 ctcctttggt gtctcaacat actccaactt tgtctctttc cacatcctcc ccactccccc
     2401 accgggcagt atagaacctc gatgttacaa gcgcgttaca gaaacgggtc gttttctcac
     2461 aggtgaatca cagtgggttc acatgaaatc tacctcgccc acaattcgtc ccaccacatc
     2521 gtccaggccg cgtcctactt ccgggctgag tccagtgccg atggcgaagc gctggccttc
     2581 gatggcgtag atcaccaggc gagatggaag ctgatccagc gcccgtgcca gctcgatggc
     2641 ttcgacgacg ccgaaggcat gcgtcgagga gaaggacagg tctgccggga gagcttgctg
     2701 atctgcgtca aagcgataga ccgtgccggg tttcctgccg gttgtgaccg cgtcgatgag
     2761 gatgaccctg tcggcgcgct tccaggtctc catcagttgg agaccatccc cctggcagat
     2821 catcaactgt gcgcccggca gcccgcgcgc tgccagggcc gcgatgatgg cggggccgac
     2881 tccgtcgtcg ccgcgcagca tgttgccgac gccgatgacc agcacctcca gtggcaatat
     2941 cgacatagcc gctcctcttc attcccgctc gacctgcagc ttcaggaagt gcgtggcgca
     3001 ggagatacaa gggtcatagt tccggatcgc ctgctcgcac tgccaggtca gttgctcttc
     3061 cggcagcgct ttgtgctgct cgacaaagcg ccacaggtcg ttttcgatgg tcttctggtt
     3121 ctgcgatgtg ggcgggatga tcttcgcgtc gcggatgatg ccgtcctcat cgaggcgata
     3181 gcggtgatac aggatgccgc gcggcgcttc cgtgcaggcg cagcccgtcg cggccaccgg
     3241 gcgaacccgg acagaggggg catccggctg ctcgtactcc tcaatgatgc gcagcgcctc
     3301 gtcgaaggcg tacaggatct ccacactgcg cacgacaatg ctctgaaagg ggttgcggca
     3361 cacctggccg agaccagcgc cacgcgcggc ttcctgcgcg agcggcgaga gctggtcgaa
     3421 attcagattg taacgcgcca tagggccgac gaaataggct cctcgcccct tcaatacgct
     3481 gtgcagcgca ttggaatacg ggacatgctc ctctacaaag tgatcctcat actcgtggac
     3541 cgggatggag aggcctttgt tcgagaccag taagccctcg ttgaagggat actcgttggg
     3601 gtgatacagc gccacgaact cgtagtcctg ctcgaagtcg gggaagggca gctgcgccac
     3661 ccagcgcacc gtctctaacg ctgcatcgcg tccccacttg agctgctcca ccagcggccc
     3721 gaactcccgc tttgccggga ccttgtaaaa tccgcccacg cgcacgttga tgggatgtat
     3781 ttctctgccg ccgaggaacg agacgatggc attgcctgtt ttcttcatct gtaaggcgcg
     3841 cttgaccagg tcggggtgat cctgcgccat gcggatcacg tcgggatagc ccaggaagtc
     3901 gggcgcgtgg agcatgtaga cgtgcagggt atggctctcg atccactcgc cgcagtagag
     3961 cagcctgcgc agcgcgcgca gttgcccccc gacggtgata ccgaaggcgt gttccatggc
     4021 gtgaacggcg ctcatctggt aggcgacggg gcagatgccg cagatgcgcg cggtgatatc
     4081 aggcgcctcg ccgaacgcgc gcccgcgcag gaacgcctcg aagaagcgcg gcggctcaaa
     4141 aatcctgaac ttgacatccg tcacctgctc gccgcttatc ttgacgtaga gcgcgccttc
     4201 gccctcgacg cgcgccaggt agtctacctt gatggtctta tcgctcatgc gcttcactcc
     4261 tcttgctatg cagctctcca acgaggggct tattccgact gacaacgcgg ctgacgccgc
     4321 tactgctgcg cagtcagtca gtcggaaagg aagactgcgt ctctaccctg cgggtcagga
     4381 gagctgatcg gaagcctttc taaatggccc ggcagcggcg ttgaagctgc ggaaaacgcg
     4441 gatgacttcc tgccttcccg cgccgagatg ctgccactga tgactcaacg aggccgtatt
     4501 gggtgtttcc tttgggccga agcagccgta gcacccgcgc gcgtaggagg ggcagatcgc
     4561 gccgcacccg gcgtgtgtga cggggccgag gcaggggatg tgctgcgcga ccatcacgca
     4621 caccgtcccg cgcagcttgc attccatgca cacgctgtgc ggcggcgtgt tcggcttgcg
     4681 cccgttcaaa taggcgctga tgacttcgag gagctggcgc ttgttgatgg ggcagccgcg
     4741 cagttcaaag tcgaccttga cgtgatcgga aatgggcgtg gaggtgttca gcacctcgat
     4801 atattgcggc gtcgcgtaga caatcgagat gaactcgttc acgtctttga agttgcgcag
     4861 cgcctggatg ccgcccgccg tcgcgcacgc gccgatggtg acgaggaaac gggaggagcg
     4921 gcgtatgtgc tgtatccgtt ccgcgtcgtg cggcgtggtg atggacccct ctactagcga
     4981 gaggtcatac ggccctttga cgacggcgcg cgaggcttca gcaaagttgg caatttcgat
     5041 ctcgccggtc agggccagta attcatcctc gcagtccagc aggctcagct ggcagccatc
     5101 gcaggaggca aatttccaga ccgccagttt gggtttgtgt tcgcgattca tgttatatct
     5161 ccgctttcac cagcaggtgc tgcacctggc tatactgaaa gaccgggccg tccttgcaga
     5221 caaagtgcgg cccgtactgg caatgtccgc acagcccaac cgcgcacttc atattgcgct
     5281 ccatcgagac gtagacgtga tcggcgacca ggccgtgttt cgccagttct gctgtggtaa
     5341 agcgcatcat gatttccggg ccgcagatca gggccagcgt attgtgcggg tcaaatactg
     5401 cccggcgaat gagctgcgtg accacgccga cactgccccg ccaggatgca tcacctcgat
     5461 caaccgtgat gtagacatcc agatcgaggc gcgctcgcca gcgctcgagt tcgtggcgaa
     5521 agagcatatc cgaaggcgtt cgcgcgccat agagtagcgc gatcttgccg tactgcgctc
     5581 gccgggcgat gagctggtac acggtggaac gcagcggcgc cagtccgatg ccgcccgcga
     5641 cgatcacgac atctttcccg gcggcctgct cgatgggcca gtgactgccg aacgggccgc
     5701 gcacgccgat agtatcgcct ggcttcaact cctgcatggc gcccgacacc gttcccacgg
     5761 cgcgggtcgt gtggacgagt ggagtgggtc tgtcgggatc gccgctgatc gatatgggga
     5821 tctcacccac gccaaagacg tagagcatgt tgaactggcc ggaggcaaac ggcggaacgg
     5881 cgctgccatc ttgcggttcc agttccattg tgaacgttcc tggtatttcg cgccgcacgc
     5941 gctgaacctg gtagagtttc ggcagcattg gatcggagag cggggtactc atacgctcgc
     6001 ctccaccgat gggacatcat agatgtcaag cgcctgcaag cgcgtggcct gcaagcgttc
     6061 catcatgatg agcgccactc gtttcaccag ctcgtagcca aagtcgtggt cggcctccgc
     6121 tttctcgcgc aggcagacgc catccagggc gatggcgcgc gtctcttcga ttgtgcgcgc
     6181 gtcgaagtgc cagcgatagg gtgggaagag ccacgaccag cccaacacct cgccctcctc
     6241 gatggtctgg atgaccaccg aaccgcgctg ggcggctgcg gcttccagcg cgactttgcc
     6301 tcggcggatg atatagaacg acgaggccgg ttcgccctcg cgaaaaatat agctctcggc
     6361 tgggaagcgc acattggaag cgcagccggt gatgagctgc atgtagcgcg gcccaaagcc
     6421 cttgaagaag ggatggttcg ccaggattgg ttcgagcgtt ttcatacctg ttcctccctg
     6481 cctcgcgccg tttcgctctc gcggatggcg cgcacttctt ccgtaatatc gatgccgacg
     6541 ggacaccagg tgatgcagcg cccgcagccg acgcaccccg atgtgccgaa ctggtcgatc
     6601 caactcgcca gcttgtgcgt catccactgg cgataacgcg acttcgacga ggctcgtatg
     6661 ctgcctccgg cgatgtagga gaagtccgta gtgaagcagg aatcccaccg gcgccagcgt
     6721 tcggcctgcg ttcctgccag gtcggtcgtg tcctcgacgg tggtgcagaa gcaggtcggg
     6781 cagaccatcg tgcagttgcc gcaggtgagg cagcgcgaag ccacattctc ccagcgcggg
     6841 tgatcgagat tgcggtagag cagccccttg atatcgctgg tctccatgct ccggcccatg
     6901 tgcgcggcag cgttcgccac agccgacccg gccaggcgga gatcatcctc gctggctgcc
     6961 ctgtaaggga catcctgtag gatggccgcc cccaggtcgg tgccgacctc gatcacaaaa
     7021 aaatgccgct cctcgtccag cacctccgtc aacgccaggt cgaagccgcc ggtcgcctgc
     7081 ggcccggtct gcatcgagac gcagaagcag gtctcacccg cctggccgca attgacggcg
     7141 atgatacaca cgttctctcg cctggttgtg tagatcgggt ccctgtaatg agcgtttgta
     7201 aaaacgcggt cctggatggc gatggcgtgg agttcgcacg aacgcacgcc gaggaaggcg
     7261 agtttctccg gctgctgctg ttcgggaagg atctgaaagc cgttctcttt gcgttcggcc
     7321 tgccataaac ggatggtggg cgggtgcagg aattgtttcc aggaatgcgg tccgacattg
     7381 tagccgaaga gcgcccggtc acggcgtttc tt
//
LOCUS       DASZAK010001089         1551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_100376, whole genome shotgun sequence.
ACCESSION   DASZAK010001089 DASZAK010000000
VERSION     DASZAK010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100376"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VGT44_19270"
     CDS             complement(<1..332)
                     /locus_tag="VGT44_19270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583009.1"
                     /translation="MENAQETDDFEIEIIDIPQDEGRNTSKALLFSLIMDFLLLLKIP
                     LPPSSCCTRIMQECQFGSVTFIGEHGTFLCTYPQLAATPSWPRGLGEVGKSILRRGAE
                     ETYTAQLA"
     gene            complement(435..998)
                     /locus_tag="VGT44_19275"
     CDS             complement(435..998)
                     /locus_tag="VGT44_19275"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV2583010.1"
                     /translation="MVIDEILAHNEQFVRQKTLPIIGHAPRKRMAVITCMDCRLVQMF
                     EQALGLERGDVLELRTAGATISQPEREGAASDLIRSLAGGIYLLGVREVAVIGHTQCG
                     LAHANPTALTASMQALGVDPQQLIERENLGNLEGLVGWLGAFSDVHINVREVVNVIRN
                     SPYLPKIPVHALVIDIETGKLEMVERG"
     gene            complement(1003..>1551)
                     /locus_tag="VGT44_19280"
     CDS             complement(1003..>1551)
                     /locus_tag="VGT44_19280"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907161.1"
                     /note="catalyzes the formation of 2-oxoglutarate from
                     isocitrate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEV2583011.1"
                     /translation="HNAWLRTIEDGIHTYDIYHDGISKQKVGTHEFSDAVIARMGMAP
                     QQLKAVNYTTAPQHASYQPVMIEHPAEQKTLVGVDVFLDWHSGSAAHLGDTFKQVSSN
                     GLELTMITNRGAKVWPDGMPETFCTDHWRCRFMARDKGAITHSQITKLLTRIDEAGFD
                     FIKIENLYNFDGKPSYSAGQGE"
BASE COUNT          292 a          430 c          484 g          345 t
ORIGIN      
        1 cacgccaatt gcgctgtgta agtctcctca gcgccccgcc gcaaaattga cttgccaact
       61 tcccccagcc cacgtggcca cgagggagta gcagccagct gcgggtacgt acacaggaac
      121 gttccatgct ctccaatgaa cgtcactgag ccgaactggc attcttgcat gatccgtgtg
      181 cagcaggaag aaggtggtaa tgggattttg agcagcaata gaaagtccat tatcaaactg
      241 aagaggagcg ccttcgatgt gttgcgccct tcatcctgcg ggatgtcgat gatctcaatc
      301 tcaaaatcat cagtctcttg agcattctcc atcggttgct ccttttgcat tttcgcggga
      361 agcactcgac caggcacgag ggcgcaatga attgtgcccc tacacaccca ttcaactttg
      421 acagggtact acgtttaccc gcgctcgacc atttccagct tgccggtttc gatatcgatg
      481 accagggcgt gaactggaat tttggggagg taggggctgt tgcgaatgac gttgaccact
      541 tcgcgcacgt tgatatgcac gtcgctgaac gcaccgagcc agccgaccag accttccagg
      601 ttgccaagat tttcgcgctc gatgagctgc tgtgggtcga caccgagcgc ctgcatggag
      661 gcggtcagcg cggtcgggtt agcatgggcg aggccgcact gagtatggcc gatgacggcg
      721 acttcgcgca cgcccagcag gtagatgccg ccggccaggg agcggatcag atcgctggcc
      781 gcaccttcgc gctcgggctg cgagatggtt gcgccggcgg tgcgcagttc cagcacatcg
      841 ccgcgttcca ggccgagagc ttgctcgaac atctgcacga ggcggcaatc catgcaggtg
      901 atgacggcca tgcgcttgcg cggcgcgtgg ccgatgatgg gcagcgtctt ctggcggacg
      961 aactgctcgt tatgcgctaa aatctcatcg atgaccatga agttattctc cctgcccggc
     1021 tgaatagctg ggcttgccgt cgaagttata gaggttttcg attttgatga agtcgaatcc
     1081 agcctcgtcg atgcgcgtca gcagcttggt gatctgcgag tgcgtgatgg cgcctttgtc
     1141 gcgcgccatg aagcggcagc gccagtgatc ggtgcagaag gtttcgggca tgccgtccgg
     1201 ccagaccttc gcgccgcgat tggtaatcat ggtcagttcc aggccgttgc tgctgacctg
     1261 cttgaaggta tcgccgagat gggccgccga gccgctgtgc cagtccagga agacatcgac
     1321 gcccaccagc gttttctgtt cggcaggatg ctcgatcatg accggctggt aggaggcgtg
     1381 ttgtggggca gtggtatagt tgacggcttt gagctgctgc ggcgccatgc ccatgcgggc
     1441 gatgacggcg tcggagaatt cgtgcgtacc gaccttctgc ttgctgatgc cgtcgtggta
     1501 gatatcgtag gtgtggatgc cgtcttcgat ggtgcgcagc caggcgttat g
//
LOCUS       DASZAK010001090         1731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_703348, whole genome shotgun sequence.
ACCESSION   DASZAK010001090 DASZAK010000000
VERSION     DASZAK010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703348"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VGT44_19285"
     CDS             complement(<1..302)
                     /locus_tag="VGT44_19285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583012.1"
                     /translation="MKTVNAYPMLSERSRGWLKYLHRKVNTPDDWDRGSHPSPMWDDK
                     STPPLLCFARFDLIDSSVGMALMAQTTPAWREVYGSILDQLLQRYGTYWGAIDWQT"
     gene            complement(501..1547)
                     /locus_tag="VGT44_19290"
     CDS             complement(501..1547)
                     /locus_tag="VGT44_19290"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEV2583013.1"
                     /translation="MSIPKTMRVAQLLAYKRPLEIREVPVPEPRGENVLIRIVGSGLC
                     HSDLHFMDGEVPIIPSFPFTLGHENAGTIAAVGENVHAVQIGDPVVVFGGWSVKSDRF
                     TRSGQEQLSDMNAWVGVGQPGGYADYLLVPTYRYLLPAQGLDLVEAAALTDAGLTPYR
                     AVKKLVPVIYPGSAVVIIGAGGLGQFGVQYARLLLPAATTVVAVDVDSQKLEIARDLG
                     ADVVINSRNEDPVQRIKELTGGEGAQGVVDFVGADTTLRQAYQSAGRQARIVIVGLAG
                     GTLPFTALSLNEIEVTTSRWGSILELSEVLALARKGLLRTRIQRVGFDEMNQTFDKLA
                     HGQIEGRAVLVPES"
BASE COUNT          380 a          500 c          480 g          371 t
ORIGIN      
        1 tgcgtctgcc agtcgatggc tccccagtag gtcccgtagc gttggagcaa ctggtcgagg
       61 atggagccat acacctcgcg ccaagctggc gtggtctggg ccatcagggc catcccaacg
      121 ctggagtcga tcaagtcaaa gcgggcaaaa cacagtagcg gcggagtgga tttgtcgtcc
      181 cacatcggcg aagggtggct cccgcggtcc cagtcgtcgg gcgtgttgac cttgcgatgc
      241 aggtatttga gccatcctcg ggagcgctcg gagagcattg ggtatgcgtt gacggttttc
      301 ataaagcttc ctcctcgttc tttgtctcca tgcgatacgt gtggaaagac cgcctgctcc
      361 ttaaaactga agagatgacg ctccaacggc agatcataca tcaccgtcga tccatcccca
      421 gactcgtttt caaagagtaa caattcgact ggacgaacaa tccactaaga tcaggccaaa
      481 caaagcgaga gacgcatagg ctagctctca ggaacgagaa cagcacgacc ctcaatctga
      541 ccatgagcca gtttgtcaaa ggtctggttc atctcgtcaa agccaacgcg ttggatgcgg
      601 gtgcgaagca gtcctttgcg ggcaagggcc agtacctcgc tgagttccag gatgctgccc
      661 cacctgctgg ttgtgacttc aatctcgttt aaggacaggg ccgtgaaagg caaggtgcct
      721 ccggccagac ccacgatgac gatgcgtgcc tgccgtcctg ctgactgata ggcttggcgt
      781 aacgtcgtat cagcccctac aaagtcgacg acgccctggg caccttcacc gccagtgagt
      841 tccttgatgc gctggacagg atcttcgttg cggctattga tgaccacatc agcgccaagg
      901 tcgcgagcaa tctcaagttt ctgggagtcc acgtctacag ccacgaccgt ggtggcggct
      961 ggaagaagca gcctggcata ttgcacaccg aattgcccca ggccacccgc cccaatgatg
     1021 accaccgcgc tgccaggata gatgaccggc acgagtttct tcacggcccg atagggcgtt
     1081 aacccggcat cagtaagtgc ggcggcttcg acgaggtcga gtccttgagc aggcaagagg
     1141 tagcgatagg taggtacgag cagatagtcc gcgtaaccac caggctgccc aactccgacc
     1201 catgcgttca tatcgctcaa ttgctcctgg ccactgcggg tgaagcggtc gcttttgacg
     1261 ctccatcctc cgaagacgac gacaggatcg ccaatctgaa cagcgtggac gttctcgccc
     1321 actgcggcga tagtacctgc attttcgtgg ccaagcgtga agggaaagct ggggatgatc
     1381 ggcacttcgc catccataaa gtgcaagtca gaatggcaga gccctgaacc gacgatacgg
     1441 atcaagacat tttcaccccg cggctcgggc acaggcacct cacgaatctc taacggccgt
     1501 ttgtaggcaa gtagctgcgc tactcgcatc gtttttggga tactcatctt tgctgttcct
     1561 tttcgggttt gtcttcgtaa acgttcactg acctcctgac tcgcctctta ttcgccagta
     1621 aatcgtggtg ggcgtttctc gaagaaggca ctgactccct cctgctggtc tttggtactg
     1681 aacaaactgg cgaaggacaa cgattccatc gtgatggcag cccctagttc c
//
LOCUS       DASZAK010001091         1708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_560217, whole genome shotgun sequence.
ACCESSION   DASZAK010001091 DASZAK010000000
VERSION     DASZAK010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560217"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1699
                     /locus_tag="VGT44_19295"
     CDS             23..1699
                     /locus_tag="VGT44_19295"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEV2583014.1"
                     /translation="MKSKESARQPAQRKRPDGGFKKRRGLQLRMTISYVAVSVATALL
                     LELLLLAIFFFVILRLPFVDPNTLKAAQQIARSYALEAAVQANGGHLDPRSTFQPGQP
                     SSLVLPGADSTESVPYTATRSSSPQTTAFALLIAPNGQVLASSYPALYPALTPAARLL
                     PDQAQLIRGALAGKAGNLIRITAQGHVLSVVQPVLNRENKPVGAIYIQMPPAFNPNGN
                     VFSFVQFWLFTALFWLLLTAPIGAIFGVLTTRSLVRRLHRLVKATGQFASGDYSQRVA
                     TTKRDEVGQLEQQFNTMAQQLVESIEQQKTLVEQHTREEERARIEQELHTAQLIQLSL
                     LPKERPTLPGWRIATYYQAAREVGGDLYDFLPFEDGRLGLVIGDVTDKGMPAALVMAS
                     TRSMLRAAAQATDSPGEVLARVNDLLYADIPEKMFVTCFYAILDPVGGRLRYANAGHD
                     LPYRQHAGSACELRATGMPLGLMPGMRYEEQEVVLARDENILFYTDGLVEAHNSGREM
                     FGFPRLQTLLAGHSGGASLIDFLLTELKGFTGEEWEQEDDLTLLTLQRTP"
BASE COUNT          344 a          563 c          475 g          326 t
ORIGIN      
        1 tctcgttaga aagaggagcg tagtgaagag caaagagagc gccaggcagc cagcgcaacg
       61 caaacgcccc gatggcggat tcaaaaagcg ccggggcctg cagttgcgta tgaccatctc
      121 atatgtggcc gtatccgttg ccaccgcgct gctgctggaa ctcctgctcc tcgccatctt
      181 cttttttgtc atcttacgcc ttcctttcgt agacccgaat acgctgaagg ccgcgcagca
      241 gatcgcgcgt tcctacgcgc tggaagcggc tgtccaggca aacggaggcc atcttgaccc
      301 tcgctccacc tttcagcctg gtcagccctc ttcgcttgtc ttaccaggag cggactccac
      361 ggagagcgtc ccctacaccg cgacgcgctc ctcttctccc cagacgaccg cgtttgcgct
      421 gttgatcgct cccaacggcc aggtcctcgc cagttcttac cctgcgctct atcccgcctt
      481 aacgcccgcc gcccgtttac tgccggacca ggcacagctc attcgtggtg ccctggcggg
      541 gaaagcagga aacttgatca ggattacggc gcaaggtcac gtgctctcag tcgtccagcc
      601 agtcttgaac cgggaaaaca aaccggtagg cgcgatctat atacagatgc cgccagcatt
      661 caatcccaat ggcaatgtct tctcgttcgt gcagttctgg ctctttaccg cgcttttctg
      721 gctccttctg acagctccaa tcggtgccat cttcggcgta ctgaccacgc ggagcctggt
      781 gcgtcgcctt caccgcctgg tgaaagccac aggacagttc gccagcggcg actactcgca
      841 gcgggtagcg actacgaaac gcgatgaagt cggacagttg gaacagcaat tcaatacgat
      901 ggcgcagcag ctggtcgaga gcatagagca gcaaaaaaca ctcgttgagc agcacacgcg
      961 cgaagaagaa cgcgcccgca tcgaacagga attgcatacc gctcagctca tccagctctc
     1021 cctcctgccc aaagaacggc ccacacttcc cggatggcgc atcgccacct actaccaggc
     1081 ggccagagaa gtcggggggg acctgtacga cttcctgcca ttcgaagatg gccgcctggg
     1141 gctggtgatc ggcgatgtca cggacaaagg tatgcctgct gccctggtga tggccagcac
     1201 ccgcagcatg ctgcgcgccg cggcgcaggc aacggactcg ccgggcgaag tcctggcgcg
     1261 ggtcaacgat ctgctctacg ccgatattcc ggaaaagatg ttcgtcacct gcttctacgc
     1321 catcctcgat ccggtcggcg gcaggctccg ctacgccaac gcgggacacg atctcccata
     1381 ccgccagcat gcgggttcgg cgtgcgaact gcgcgcgacc ggtatgcccc tcggcctgat
     1441 gcccggcatg cgctatgaag aacaggaagt ggtcctcgcc cgcgatgaaa acatcctgtt
     1501 ctacaccgac ggcctggtgg aggcgcataa ctccgggcgc gagatgttcg gctttcctcg
     1561 tctccagacc ttgctcgctg gacactccgg cggggcatcg ctcatcgact tcttgctgac
     1621 cgaactcaaa ggcttcaccg gcgaggagtg ggagcaagag gacgacttga ccctgctgac
     1681 gctgcaaaga acgccctgac aactcctc
//
LOCUS       DASZAK010001092         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_419146, whole genome shotgun sequence.
ACCESSION   DASZAK010001092 DASZAK010000000
VERSION     DASZAK010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419146"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..862
                     /gene="ccsA"
                     /locus_tag="VGT44_19300"
     CDS             119..862
                     /gene="ccsA"
                     /locus_tag="VGT44_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEV2583015.1"
                     /translation="MAITKHETAAGQRASATTARPHTARFPLVSLLLGALSFAGMLVS
                     IWMIFLYAPMDAVQGQPQRIFYFHVPIAWLAMLGFGVVAVGGIGYLITKNESWDWAAR
                     AAAELGALFATLTLITGSIWGKTIWGTWWTWDARLTTTLILWFIYIGYLMLRSYMGRT
                     PESARAGTVLAIIGVIDVPIIYESVNWWRTLHPAQQVGVAGALPPSVVLTLMVSLTSF
                     TLLFAFLMIQLYQLQRMQTLAQRLRANLM"
     gene            876..1043
                     /locus_tag="VGT44_19305"
     CDS             876..1043
                     /locus_tag="VGT44_19305"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HEV2583016.1"
                     /translation="MDGTYYLVAAYIIAWVGLFAYLAFVMLRIRGVRTELAAVEELVR
                     EQSEKQPDHSV"
     gene            1061..1504
                     /locus_tag="VGT44_19310"
     CDS             1061..1504
                     /locus_tag="VGT44_19310"
                     /inference="COORDINATES: protein motif:HMM:NF022700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HEV2583017.1"
                     /translation="MAAKKRVYAAERPYRQSVILLVAGDIMCFLIFSIVGDASHNEFS
                     GLAAVPHIIAITLPFLAGWFLVAPFFGLFRKEMTTAPRAMAYRTAFAWIPAWIIAMLL
                     RGLFIDHAVPGSTFMLIAFVFNLAVLELWRWPFALNNLARKQSVK"
     gene            1796..>2193
                     /locus_tag="VGT44_19315"
     CDS             1796..>2193
                     /locus_tag="VGT44_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583018.1"
                     /translation="MDIHQPSSTQGIDPAALPPTRPKRSRKLGQRAAQVALAFLLCLG
                     IFLSLLPWGRAFTRGALLLPALLSASAPAPLVLAGDPVRFTRLTLFPNTARTVYLDIY
                     EPATPPPPIPGGREAIIEVPGAGDNRTVPQL"
BASE COUNT          414 a          712 c          583 g          484 t
ORIGIN      
        1 ccccgtccat caacgagccg ccctggcttg gcctgatcac cgccttcgat gtcatatttt
       61 tgagcgtcgc catgatgacg tttgaattcg tcgtagagga ataattcgga gcaatactat
      121 ggcaattact aaacatgaaa cagcagccgg gcaacgcgca tccgcaacaa ccgcgaggcc
      181 acacaccgcg cgcttcccgc tggtatcgct gctgctgggc gcgctctcct tcgcggggat
      241 gctcgtctca atctggatga tcttcctgta cgcgcccatg gatgccgtgc agggccagcc
      301 gcagcgcatc ttctattttc acgtgcctat cgcctggctc gccatgctcg gtttcggcgt
      361 cgtggctgta ggcggtattg gctaccttat caccaaaaat gaaagctggg actgggccgc
      421 ccgcgcggcg gctgaactgg gcgcgctctt cgccacactg acgctgatta ccggatcgat
      481 ctggggcaaa accatctggg gaacatggtg gacatgggat gcgcgcctga ccacgacgct
      541 gatcctctgg ttcatctaca tcggctacct gatgctgcgc agctacatgg gtcgcacgcc
      601 cgaaagcgcg cgcgccggca ccgtgctggc catcatcggc gtcatcgatg ttcccatcat
      661 ctacgaatcg gtcaactggt ggcgcactct gcaccccgca cagcaggtag gcgtggctgg
      721 cgcgctgccg ccctccgtcg tcctgaccct gatggtgtcg ttgacatcgt tcaccctgct
      781 cttcgctttc ttgatgattc aactatatca attgcagcgt atgcaaaccc tggcacagcg
      841 tttgcgcgcc aatctcatgt aaggataaca tcaccatgga cggaacatac tatctcgtcg
      901 ccgcctacat catcgcctgg gtcggcctct tcgcctacct cgccttcgtc atgctgcgca
      961 ttcgcggcgt gcgcaccgaa ctggccgccg tcgaagaact cgtgcgcgaa caaagcgaga
     1021 aacaacccga ccattctgtt taaataaagg agcaagcagc atggctgcca aaaaaagagt
     1081 ttacgcggcg gagcgaccgt atcgccaatc ggtcatcttg ctggtagctg gcgatatcat
     1141 gtgctttctg atcttctcga ttgtgggcga tgcgtcacac aatgaatttt cgggactggc
     1201 tgccgttccg cacatcattg ccatcacgct cccctttctg gcaggctggt ttctcgtcgc
     1261 gccattcttc ggcctgtttc gcaaagaaat gacgactgcc ccgcgcgcca tggcctaccg
     1321 cactgcgttc gcctggatac ctgcctggat catcgccatg ctcttgcgcg gtctcttcat
     1381 tgatcacgcc gtacccggat cgaccttcat gttgatcgcc ttcgtcttca acctggcagt
     1441 cctcgaactc tggcgctggc cattcgccct gaataacctg gcgcgcaaac aaagcgtgaa
     1501 gtaagggcat acagatatcg tcggcgtatt cacagaggcg accaggcgtt gtagttttta
     1561 cctggcttcc atcgatggat tctacgcttt agcaacatgg ccatcgccct ggtcatcgcc
     1621 acatgacact gatgcctgat ctccgcatca gtgacctgct gacgcgctga agcgtcttat
     1681 cctctaggaa agaaaagcag tatgcacacg tctgtcggct cacgtgctgg tcgcccggcg
     1741 acagcgcctg gacgagccgt gtcaaccggc acgagaagcg cgagacggca ctgcaatgga
     1801 cattcatcag ccatcttcca cgcagggaat cgatccggct gcgctgccac caaccaggcc
     1861 gaagcgcagc cgaaaactgg gccagagagc agcgcaggta gctctcgcct tcttgctgtg
     1921 cctcggcatt ttcctttcgc tgcttccctg gggccgggct tttacgcgcg gtgccttgct
     1981 gctcccggcg ttactcagcg cctcagcgcc cgcgccactt gtgctggccg gcgaccctgt
     2041 gcgcttcacg cgcctcacac ttttcccgaa cactgctcgc acagtttatc tggacatcta
     2101 cgagccggct acgcctccgc cgccgatccc cggcgggcgt gaagccatca tcgaagtgcc
     2161 gggcgccggc gataaccgca ccgtcccaca act
//
LOCUS       DASZAK010001093         1738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_240410, whole genome shotgun sequence.
ACCESSION   DASZAK010001093 DASZAK010000000
VERSION     DASZAK010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240410"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VGT44_19320"
     CDS             <1..867
                     /locus_tag="VGT44_19320"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2583019.1"
                     /translation="SQLFRIVGTLQSKGSQGFANSDDVIFVPYSAANARLKPSPLYVD
                     QIQVLVDDINNITQVQQEITTLLRARHHLTGPAPSTSSQPIAGFGAGAGGGVVVGKGG
                     GGAGGLGGAGRGSAGGGSGLGSGAGRATPGAKTGAGQANDFQIINGSQLVQTAQQSTA
                     ELTILLVGIAAISLTVGGIGIMNIMLVSVTERTRDIGICMAIGARQRDIRNQFLLEAL
                     LLSVIGGLIGIMLGLGFGFELTSKIGFPFVFNPSALALAFGVSAVVGIVFGLYPAVRA
                     SKLDPIVALRTE"
     gene            complement(938..1423)
                     /locus_tag="VGT44_19325"
     CDS             complement(938..1423)
                     /locus_tag="VGT44_19325"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEV2583020.1"
                     /translation="MAANSQTETKTLRVGDVAPDFSLKTGKGETWRLADFRDKKNVVL
                     AFVPFAFSSVCSAQIPSYEAELERFKDYDTEVVSVSTDSGHALRAWAKSMGTSIANLS
                     DFYPQGQVADLYGVRNAGGFSNRVVVLIDKKGIIRFIEFMNSPGDSTDNEALFEALRK
                     L"
BASE COUNT          377 a          488 c          482 g          391 t
ORIGIN      
        1 agccagctct tccgcattgt tgggacgctc cagtcaaagg gttcgcaggg ctttgccaat
       61 tcagacgacg tgatttttgt gccatatagc gctgcaaacg cgcgtctgaa accgtctccg
      121 ctctatgtcg atcaaatcca ggtgctggtc gatgacatca acaatatcac gcaggtacag
      181 caagagatca caacgctcct gcgggcgcga catcacctca caggtcccgc cccatccact
      241 tcaagtcagc ctatcgcagg ttttggagca ggcgctggcg ggggcgtagt agtaggcaag
      301 ggaggcggtg gcgcaggagg attaggtggc gcggggcgtg gatcagccgg cggtggaagc
      361 ggattgggta gtggcgcggg acgtgcaaca cccggggcga aaactggagc ggggcaagcc
      421 aatgacttcc agattatcaa tgggagccag cttgtccaga cagctcagca gagtactgcc
      481 gaactgacca tcctgctcgt cggtatcgct gcgatctcgc tgaccgtcgg tggcattggt
      541 atcatgaaca tcatgctggt ttctgtcacc gagcgcaccc gcgacatcgg tatctgtatg
      601 gcgattggcg cgcgccagcg cgatatccgc aaccagttcc tgctggaagc cttgctgctg
      661 agtgtcatcg gaggactgat cggcatcatg ttaggattag gcttcggctt tgaactcacc
      721 agtaaaattg gctttccctt tgtcttcaac cccagcgcgc tggccctggc ctttggcgtt
      781 tcagccgtcg ttgggatcgt cttcggcctt tatcctgctg tccgcgcgtc caaactcgat
      841 cccatcgttg cgctgcgaac ggaataagaa aagaagatgt gaatgccagg caagaccgct
      901 gcgcggtctt gcctggcatt cacatcttct ttttacttta cagtttgcgc aatgcctcga
      961 aaagcgcctc gttgtccgtc gaatcgccag ggctgttcat gaactcgata aaacggatga
     1021 tacccttttt atcgatgagg accaccacgc ggttcgagaa gccgccagcg ttccgcaccc
     1081 catacagatc ggcaacctga ccctgtggat agaagtcgga tagattggcg atggaagttc
     1141 ccatcgattt cgcccaggct cgcagcgcat gtccgctatc ggtgctgaca ctgaccacct
     1201 cggtatcgta gtccttgaag cgttccagct ccgcctcata agagggaatt tgcgccgagc
     1261 agaccgagct gaacgcgaat gggacgaagg ccagtaccac attcttctta tctcggaagt
     1321 ctgccagccg ccaggtttcc cctttaccgg ttttcagtga gaaatcgggt gccacgtctc
     1381 cgacgcgcaa tgtcttagtt tcggtctggc tattcgctgc catacactgt tattcctttc
     1441 tcgacctgtg cgctgccatg cgccaggcca caatcgatcg acttgccgac gttacttgcc
     1501 acaaacacga aacgtcatgc aagtgtgata tttctggttt gcaccgggta tgcatttgca
     1561 aacaacaccc acattcggga ttttagcact cgcgaaaagc gcgtgtcaaa cacaacagag
     1621 ggaaagcgca ctcttgcatg ttacaagaag aagtcactca accgcggcca ccggaggagg
     1681 cggagtctaa cctggcgaaa gagaaatggc tggcgcgcaa cgggacgctt gtctatcg
//
LOCUS       DASZAK010001094         5208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_384445, whole genome shotgun sequence.
ACCESSION   DASZAK010001094 DASZAK010000000
VERSION     DASZAK010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384445"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1418
                     /locus_tag="VGT44_19330"
     CDS             <1..1418
                     /locus_tag="VGT44_19330"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2583021.1"
                     /translation="PNFTMSPCAYVVSLLRPEIIRDLELHRYGFDAYVKDPQMFVPFL
                     DGKYLFFRASTEKTIEGIRKLSKRDAEAYPKFLRFFERASEILNPILLEEPPSVANLA
                     ARFRGEDEEIYRYLMFGNLYDMLADYFESDYLRAAWAGQGVIGSFIGPRTPGSVYVAW
                     HHMFGEVNGNPGMWGYVRGGMGRISFAMAASAEAHGAEIRTNTRVAKILTHNSKAEGV
                     RLENGDEVRAAAVLSNADPKRTFLQFCQDADLDKDFLKRIARFKTESAVIKLNIALKE
                     LPSFTCLPGTKPSLQHAGSLDISPTPDWVQNAYEDAMRGALSQKPYIEAYMQSATDPS
                     VAPAGYHTISMFCQYAPYHLRGREWSDAVKNEMADRIISTVTEFAPNFADAVLHRQVL
                     SPVDIEQRYGMPNGNIFHGEITPDQLFSLRPAPECARYRTPLEGLYLCGSGTHPGGGV
                     MGAPGHNAAKALLSDWAKKLP"
     gene            1528..2373
                     /locus_tag="VGT44_19335"
     CDS             1528..2373
                     /locus_tag="VGT44_19335"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEV2583022.1"
                     /translation="MRFKVAVVQFEISQFEPEANLAKAEEFIKQAASASADLIVFPED
                     VVVGPLNGRANLADFDGRYVKHFQQLADQYSIDIVPGSIIEGDETGLYNTTYYIDHTG
                     DILGKYRKVHLWLPERGYITPGEEVCVVETRFGKVGLIICWDLMFPETFRAMIEEDVE
                     IVICPSYWCLEDAGDGQRLNRFAEVTLVNALCIARAFEQEIVLIYANAAGKTVIDDNI
                     CTLVGQSQITVPFQGPLDIAMHNDEVMFIQEVDTSVLAIAEQAYEIRNDLRSGQVSSK
                     ASLIH"
     gene            2383..3129
                     /locus_tag="VGT44_19340"
     CDS             2383..3129
                     /locus_tag="VGT44_19340"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV2583023.1"
                     /translation="MNNHNLNKRPHVPDIELKAVLFDLDDTLFDHRYSSRASLSAISQ
                     QHTCLQSSTLDELEQMHRQFLEDAHLQMLAGHLSLDAARVIRLQRLFEQHGERIAPAM
                     AVEISRESRRHYQAARRAVPGAIALVEALRSRLKIGIVTNNLSEEQQEKLRHIQLAPL
                     IDVLVISEEVGVTKPDPAIFHIALERLVCQACEVVMIGDSWSTDIIGAHQLGIRAIWL
                     NRYGVDCPDPAMATEIASLEPLEEVLALLF"
     gene            complement(3154..3558)
                     /locus_tag="VGT44_19345"
     CDS             complement(3154..3558)
                     /locus_tag="VGT44_19345"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEV2583024.1"
                     /translation="MCNFLQLYGTSYLFVRITLTGPLPENVHVECYIPHALLLPHCSA
                     VICHGGPGTTLNSLALGLPLLILPQGADQFVVGDLVRASGAGLCLTPQHVNASTVRQS
                     VLALLDEPGYRANARRLQREIIAMRGLRRQSN"
     gene            4063..4455
                     /locus_tag="VGT44_19350"
     CDS             4063..4455
                     /locus_tag="VGT44_19350"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2583025.1"
                     /translation="MLNHVSDLDSVFHALADPGRRLMLERLGQGPASVSELGKSLPMT
                     LAAVVQHVQVLEASGLVRTQKLGRTRMCSLNLVPLRSAERWISERRAFVERSLGRLGQ
                     YLAETTETTDPPTNAGQPGLHPSEEETS"
     gene            4452..4931
                     /locus_tag="VGT44_19355"
     CDS             4452..4931
                     /locus_tag="VGT44_19355"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEV2583026.1"
                     /translation="MTEQPVIHNTFVIERTYPTPVSRVFAAFTTKEAQDIWRDTGDLD
                     APESSINGGDSEFDFRVGGHERFGFGYQGVSQRYDATYYDIVPERRIIYSYEMYADDV
                     RSSVSVATIEFVPVDGGTALTWTEQGAYLNGVDGDEAPHLRRGGTSEMLDGLAKYLG"
BASE COUNT         1161 a         1518 c         1447 g         1082 t
ORIGIN      
        1 tcccaaactt cacgatgtcg ccatgcgcct atgtggtgag cctgttgcga ccggagatta
       61 tccgtgatct cgaactgcac cgctacggtt ttgatgcgta tgtgaaagac ccgcagatgt
      121 ttgtgccgtt cttagacggt aagtaccttt ttttccgcgc cagtaccgaa aaaaccattg
      181 aaggcatccg caagctctcc aagcgcgatg ccgaagcata cccgaaattc ctgcgtttct
      241 tcgagcgcgc ctcggagata ctcaacccta ttttgctgga agagcctccc tcggtcgcga
      301 acctggcggc tcgttttcgt ggcgaggatg aagagatcta tcgctatctg atgtttggca
      361 acctgtacga tatgctggct gactacttcg agtctgacta tttacgtgct gcctgggcgg
      421 gtcagggagt gattggttcg tttatcggac ccaggactcc gggttcagtg tacgttgcct
      481 ggcatcatat gttcggcgag gtgaacggaa atccgggcat gtggggatat gtgcgcggcg
      541 gcatgggtcg catcagcttt gctatggcgg cttccgccga ggcgcatggc gcggaaattc
      601 gcacaaacac gcgcgtcgcg aagatactca cccataacag caaagctgag ggggttcgcc
      661 tggaaaatgg cgacgaggtg cgcgcggctg ccgtcctctc caatgcagac ccgaagcgca
      721 ccttcctgca attctgccag gacgccgacc tggataagga cttcctcaaa cgcatcgcgc
      781 gcttcaagac ggagagcgcg gtaatcaagc tgaatatcgc gttgaaagaa ctgcccagtt
      841 tcacctgcct gcctggcaca aagccatcct tgcagcacgc cggttcgctc gatatttcac
      901 caacgcccga ttgggtgcag aacgcctacg aggatgccat gcgcggcgca ctttcgcaaa
      961 agccctatat tgaggcgtat atgcaatcgg ccaccgaccc atccgtcgct ccagcaggct
     1021 atcacaccat ctcgatgttc tgccagtacg cgccctacca cctgcgcggg cgcgaatggt
     1081 cggacgcggt caagaacgag atggcggatc gcatcatctc caccgtgacc gagttcgcac
     1141 cgaactttgc cgatgccgtg ctgcatcgcc aggtactcag ccccgtcgat atcgagcagc
     1201 gctatggtat gcccaacggc aatatttttc atggcgagat cacgcccgac cagctcttct
     1261 cgttgcgacc cgccccggaa tgcgcgcgct accgcactcc tctagagggt ttgtaccttt
     1321 gcggatcagg cacccatccc ggcggcggcg taatgggggc tcctgggcat aatgccgcaa
     1381 aagcgctgct cagcgattgg gcgaagaaat tgccatgatg cccgaaagtc ccgctcatct
     1441 tggctcgcta gcaggcgtat agtatactta cattatgcgt aaatatacga tgaacgatgc
     1501 ataatggaca cttttcaagg atatactatg cgattcaagg ttgctgtcgt ccagtttgaa
     1561 atcagccagt ttgagccaga ggcaaacctc gcgaaggcgg aagagtttat caagcaagcc
     1621 gcctcggcat cggctgacct gattgtgttt cctgaggatg ttgtcgttgg accgctcaat
     1681 ggtcgcgcca acctcgcgga ttttgatgga cgctatgtga agcattttca gcaactcgca
     1741 gatcaatata gcatcgacat cgttcccggc tcgatcatcg agggagacga gactggcctg
     1801 tacaacacca cctactatat cgatcacacc ggcgacatcc tgggaaaata ccgtaaggtg
     1861 catctctggc tcccggaacg cggctacatt acgccagggg aagaggtctg tgtcgtcgag
     1921 acgcgcttcg gcaaggtcgg tttgattatt tgctgggatt tgatgtttcc cgagaccttc
     1981 cgtgcgatga ttgaggaaga cgtggaaatc gtgatctgcc ccagctactg gtgcctggag
     2041 gacgcaggcg acgggcagag actcaaccgc ttcgccgagg tgacgctggt caacgcgctg
     2101 tgcatcgcca gggccttcga gcaggagatc gtgctgatct acgccaacgc cgccggcaag
     2161 acagtgattg acgataatat ctgcaccctg gttggacaat cccagatcac cgtgccattt
     2221 caaggaccgc tcgatatcgc catgcacaat gacgaagtca tgtttattca agaggttgat
     2281 acctccgtgc tggccatcgc cgagcaagcc tacgaaatcc gcaacgacct gcgcagcggc
     2341 caggtcagct cgaaagcatc gctcatccat tagcacactt cgatgaacaa tcacaacctc
     2401 aacaagcgac cccacgttcc cgatatagaa ctcaaggccg tactgtttga cctggatgat
     2461 accctcttcg atcaccgcta tagcagccgc gccagcctct cggccatcag ccagcaacat
     2521 acctgcctgc aaagcagtac gctcgacgag ttggagcaga tgcaccgcca gttccttgag
     2581 gatgcgcatc tccagatgct ggcaggccat ctttcgctgg acgccgcgcg agtgatccgt
     2641 ttgcaacggc tgttcgagca gcatggcgag cgcattgctc ctgcgatggc cgtagagata
     2701 tcccgggagt cacgcaggca ttaccaggct gctcgccgtg ccgttccagg agctatcgcg
     2761 ctggtagaag ccctgcgatc tcgcctgaaa atcggcatcg tcaccaacaa cctgtcggaa
     2821 gagcagcagg agaaattgcg tcacatccag ctcgccccgc tcatcgatgt actggtcatc
     2881 tccgaagagg taggcgtgac caaacctgac ccagccatct ttcacatcgc gctcgaacgc
     2941 ctggtatgcc aggcctgcga agtcgtcatg atcggcgatt catggtcgac ggatatcatc
     3001 ggcgcgcacc agctcggcat ccgcgctatc tggctcaatc gctacggcgt agactgccct
     3061 gatccagcga tggctacgga aattgcatcg cttgaaccgc ttgaggaagt gctagcattg
     3121 ctgttttagc cggtcaactg gcgatctcct ggatcagttg gactgcctcc tcaggccccg
     3181 catagcgata atctcgcgct gcaagcgacg ggcgtttgct cggtagccgg gttcatccag
     3241 gagagcgagc acgctttgcc gcacggtcga tgcgttgacg tgctggggcg tcaggcaaag
     3301 cccggcaccc gatgcgcgga cgaggtctcc gacgacaaac tgatcagctc cctgcggcag
     3361 aatcagcaga ggaaggccca gcgccagcga atttaacgtg gtaccaggac cgccgtgaca
     3421 gatcaccgca gagcagtgtg gaagcagcag tgcgtggggt atgtagcact ctacatgcac
     3481 attttcaggc aatgggcctg tcagtgtgat tctgacgaaa agatacgacg taccatataa
     3541 ctgaaggaag ttacagatcg ctttcctctt ctaacgacag cgagaattgg cctttcggta
     3601 gtttttcgac gataggatag cgtgtacctg ataagtagcc cctttagagg tcattacctg
     3661 tcgcatccgc aagtaagact gacccgagga ttccaccaac tatcccagca aacaccccaa
     3721 tgattcctgc aacctcacct gctgttgaaa tggctgatgg gattgggata ctgaccagct
     3781 atccatccac aaagaacgaa ataaatccca tggtcctcgc gtgggcttgc ttcctgatta
     3841 cctgttggga atgagcaagc gcatatgtcg cgggtcaagt tgtgagcacg ttggtgggca
     3901 gtgtggtgaa gtgggaactt gacttcagtc atagccacga ttggcgacag catgacgcgc
     3961 gggagaggtg aatagcggat ggatgagggg cgacgctcta ctgagggcaa atacttaagc
     4021 taacccttga ctattcagca aagtcagggt atactaaagc atatgctaaa ccatgtatcg
     4081 gatctggaca gcgttttcca tgcgctcgcc gatcctggtc gccgactcat gctggaacgg
     4141 ctcggccagg gtccagcttc ggtcagcgaa cttggaaagt cgttacccat gacgctcgct
     4201 gcggttgtgc agcatgtgca ggtccttgag gcaagcggtc tcgtgcggac gcagaagctt
     4261 ggtcgcaccc gcatgtgcag cctcaacctg gtgcccttac ggtcagccga gcgctggatc
     4321 tctgagcgac gcgccttcgt cgagcggagc ttgggccgtc tcggccagta cctggctgag
     4381 accactgaga ccaccgaccc accgacgaac gccggacaac ccggccttca tccatcagaa
     4441 gaggagacct catgacagag caacctgtta tccacaacac attcgtgatc gagcggacct
     4501 acccgacacc tgtgtcacgc gtgttcgccg cctttaccac gaaggaggcc caggacatct
     4561 ggcgtgacac gggcgaccta gacgcacccg agtcgagcat caacggcggc gactcagagt
     4621 ttgacttccg ggtcggcggt cacgaacgct tcggcttcgg ctatcagggt gtctcccagc
     4681 gctatgatgc cacctactac gacatcgtcc cggagcggcg aatcatctac agctacgaga
     4741 tgtacgccga tgatgtgcgc agctccgtct ccgtcgccac catcgaattt gtcccagtgg
     4801 acggcggaac cgcgctgacg tggaccgagc agggcgccta cctcaatgga gtcgacggcg
     4861 atgaggcgcc gcatctgcgc aggggaggca ccagcgagat gctcgacggt ctcgccaaat
     4921 acctgggcta agacgcgatc acgagaccga cggaacgagc cagagaagaa cactatgcgg
     4981 gcagtgatag cgcgaatctt cgactattca ctcgacggca tcgtcgccga ggaaggcacc
     5041 agcttcttcg acttctgttg tacacatcat ggggcgcaaa cactaccaag acgctgcgca
     5101 gtattttcct gaggcagtcg accatcccta cgccgacgtc ctgaacgccg cgcgcaaggt
     5161 catcttcagc cagaccatcc agaccatcga ctgggctaac tcctccat
//
LOCUS       DASZAK010001095         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_62735, whole genome shotgun sequence.
ACCESSION   DASZAK010001095 DASZAK010000000
VERSION     DASZAK010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62735"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1081
                     /locus_tag="VGT44_19360"
     CDS             173..1081
                     /locus_tag="VGT44_19360"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV2583027.1"
                     /translation="MTNIDPFLVSGMSGDERVHVFRRVFDVQGAFDGLEVDAYIVTTE
                     RYIVMLDTMLFPDDAAAMMQQVVISRMERSVLGVNSHADWDHAWGNAYFSGTNAAPII
                     AHDHCRERLLSEEARQELDEFRQINASFQQVRLVAPTITFSESLTIDGGDLTIELLTA
                     PGHHLDEIVAWIPELRLLLAFDAVEYPLPSVDGAQGVPLMFATLERLIALEPRRVLCS
                     HGKTTDPQLIQTNLAYLREIERRCNVYLGDETRPLQQVDTPPSQLIAFPFDEVIAGHD
                     GEFDRTFYSQAHEENIDAVLRWIKDL"
     gene            complement(1086..>2092)
                     /locus_tag="VGT44_19365"
     CDS             complement(1086..>2092)
                     /locus_tag="VGT44_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583028.1"
                     /translation="DGVYMSADGGLHWQRRSAGLPTPSAIYALAFDDSGKKLFAAADT
                     GLFFSTNAGQSWMIMSHLPNDGYSSIAFDLKAAQSIFVASRHHGLLYSSNGGLSWASI
                     SAGLPVSMTINSLTFDSNQHQLWVATDQGIYLSSNNGSSWLAANIGLPTGASINDVLP
                     ASVYGGNPDVIYAGTSQGFYLSQDQGAHWMPSQTALVRVNILAILIDVQSASTVYIAT
                     DKVGVLRSTDNGQNWSSVAPGLPTTKPVYAIVQGATNYSQLFAATNDVYLFSGTGSIF
                     DPTRLLPLLLVVVFFLLLIRLSSRSRRGARNMLKPERIIESNGTPTDEAKPDNPPHKA
                     "
BASE COUNT          437 a          560 c          628 g          467 t
ORIGIN      
        1 cggcaggccc tgaatacccg ccgagcctgg aggctcacgt gctacggcgc catcatgctg
       61 gtgtgcctga tgctgttcgc gctctacttt gtggtggcga taatcggtgg gcgtcactga
      121 tattattgca acaatattga cggtagatat cagcggatag gggagcaatc gaatgaccaa
      181 cattgacccg tttctggttt cgggcatgag cggtgatgag cgtgtacacg tctttcgccg
      241 cgtttttgat gtacagggtg cgtttgatgg gctggaagta gacgcttaca tagtgacgac
      301 cgaacgctac atcgttatgc tggataccat gttgttccca gacgatgcag ccgcgatgat
      361 gcagcaggtc gtcatatcac gcatggagcg cagtgtactc ggcgtgaaca gccacgccga
      421 ttgggatcat gcctggggca acgcttattt tagcgggacg aatgctgccc caattatcgc
      481 ccatgaccac tgccgtgaac gtctcctatc cgaggaagcc aggcaagaac ttgatgaatt
      541 tcgccagatc aacgcgtctt ttcagcaggt acgcctggtt gcgccgacca tcaccttctc
      601 ggaaagcctg accatcgatg gtggtgattt gaccattgaa ctgctgaccg cgcctggcca
      661 tcacctcgac gagatcgtag cctggattcc tgaactgcgc ctgctgttgg ctttcgatgc
      721 tgtcgagtat cccctgccta gcgttgatgg agcgcagggt gtgccgctca tgttcgccac
      781 gctggaacgg ctcatcgcgc tggaaccccg gcgggtgctc tgctcgcatg gcaagacgac
      841 cgatccccag cttatccaga cgaacctggc ttacctgcgc gagatagagc ggcgctgcaa
      901 cgtctatctt ggtgatgaaa cccgccctct gcaacaggta gacacaccac cttctcaact
      961 catcgcattc ccattcgacg aagtgatcgc cggtcatgat ggcgagttcg atcgtacgtt
     1021 ttacagccag gcgcacgaag agaatatcga cgctgtgctg cgctggataa aagatctata
     1081 gtccgtcaag ctttatgggg agggttatcg ggtttagcct cgtcggtcgg cgtcccattt
     1141 gactcgatga tgcgttccgg tttcagcatg ttgcgggctc ccctgcgact acgagaactc
     1201 agccggatca aaaggaggaa gaatacgacg actaggagca gcggaagaag ccgggttggg
     1261 tcgaaaatgc tgcctgtgcc ggagaacaag tagacatcgt tcgtcgcggc aaacagctga
     1321 ctgtagttcg tcgcgccctg gacgatggcg tagacgggtt tggttgtcgg caagcccggc
     1381 gcgacgctgc tccagttctg cccgttatcg gtactgcgca gcacgcccac tttgtccgtc
     1441 gcgatataga ccgttgaggc gctttgtaca tcgatcagga tggcgaggat gttcacacgc
     1501 accagcgcgg tctggctggg catccagtgc gcgccttgat cctgagagag gtaaaagccc
     1561 tggctggtgc cggcgtagat aacatccggg ttgccgccgt agacggaggc gggcagcacg
     1621 tcgttgatgc tggctcccgt gggcagtcca atattcgcag ctagccagct agagccgttg
     1681 ttgctggaaa gataaatgcc ctggtcggtc gcgacccaga gctggtgctg gtttgaatcg
     1741 aaggtaaggc tgttgatggt catgctcacc ggcagacccg cgctgatcga tgcccaggat
     1801 aggccgccgt tggagctgta aagcaggcca tgatgcctcg atgcaacaaa gatggattgg
     1861 gctgctttga gatcgaaagc gatagaggaa taaccgtcgt tgggcagatg cgacataatc
     1921 atccaggatt gacctgcatt tgtgctgaag aagagacctg tatcggcggc agcaaagagt
     1981 tttttcccag aatcatcgaa tgccagcgca tagattgcgc tgggggttgg cagacccgcg
     2041 ctcctgcgtt gccagtgcaa cccgccgtcg gcgctcatgt agacgccatc ct
//
LOCUS       DASZAK010001096         4516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_455866, whole genome shotgun sequence.
ACCESSION   DASZAK010001096 DASZAK010000000
VERSION     DASZAK010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455866"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..778
                     /locus_tag="VGT44_19370"
     CDS             158..778
                     /locus_tag="VGT44_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEV2583029.1"
                     /translation="MEIGFFLRSLLIGLSIAATVGPMSILCIQRTLQKGYVYGLVSGL
                     GVATADGCYGSVAAFGLTIISTFLVSQQSWIRLIGGLFLVYLGTRTILSRPPERAAVP
                     KAGVGTLLGAYTSTFLLTLTNPLTILSFAAIFAGLGVASTGRNYLAATLVVAGVFCGS
                     ALWWCILTGGISLLRARFTTTWMLWLNRLSGCIIAAFGIIALVSLI"
     gene            complement(790..1734)
                     /locus_tag="VGT44_19375"
     CDS             complement(790..1734)
                     /locus_tag="VGT44_19375"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEV2583030.1"
                     /translation="MCETCGCQGGIISRAASETSTETTTSESAPQPFAERLSDRLQQI
                     IGVLVGANRKPPRRFKSFLNGSWLGHPLHPALTDVPIGAWLLTVVFDVTWLVSSNAWA
                     ARGAEAAVIVGILGALGAAATGLTDWSDTYGAERRVGLNHAIFNVSALVLYIISLVLR
                     LIQPAGDSISGAIFGFAGFILVAYAAYLGGDMVYTKGTNVNHTAWETGSDTFEAIMPL
                     EQVEEGKLFRVMASGVPVVLVRHDSQVFALSATCTHAGGPLDEGTLEGDVVQCPWHGS
                     RFSIRDGRVLAGPATAREPRYEVRVNNGQVEVRRASDQ"
     gene            1883..2503
                     /locus_tag="VGT44_19380"
     CDS             1883..2503
                     /locus_tag="VGT44_19380"
                     /inference="COORDINATES: protein motif:HMM:NF016581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEV2583031.1"
                     /translation="MNWYRRFFLSTRGRILSLIRRESRTVDDLADALGLTDNAVRAHL
                     AALERDGFVQQRGARKGSSKPAFVYELAEGAEPLFPKAYGQILNELLAVLDKQLNAEE
                     LEELLRATGRHIAARWNRLSGSTYARLEAAVEILNELGGLADLEPLNGGYCIRSNSCP
                     LAAVVPDHPAACHLAETLIAELVGVPVQERCEKGDSPRCRFEVTPI"
     gene            complement(2570..3808)
                     /gene="rpoD"
                     /locus_tag="VGT44_19385"
     CDS             complement(2570..3808)
                     /gene="rpoD"
                     /locus_tag="VGT44_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV2583032.1"
                     /translation="MVNEEVLLEKVNTEPLHFVEGAFEVSILDGNADFDMNTLSGHTH
                     AATGGSFVTPVDTTESFDLFKLQDDESMDTLDPNKEPPVDWEPGPEDEIDVEAEAQDK
                     WDELHDGLGIVMADLESTLDDPVRMYLREIGRVPLLTGEEEVRLARRMERGRTELRKP
                     ALMRDYRLIEDGEEAQRRLTEANLRLVVSVAKKYIGRGMSLLDLIQEGNIGLIRAVEK
                     FDYVKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINRLIRISRRLLQDLGR
                     EPTSEEIAEQMEISPEKVREIIKVSQEPVSLETPIGEEEDSHLGDFIEDHTALAPADA
                     ASHQLLKEQVEDVLASLTERERKVLQLRFGLDDGRSRTLEEVGKEFHVTRERIRQIEA
                     KALRKLRHPSRSRKLKDYLD"
     gene            complement(4018..>4516)
                     /locus_tag="VGT44_19390"
     CDS             complement(4018..>4516)
                     /locus_tag="VGT44_19390"
                     /inference="COORDINATES: protein motif:HMM:NF012974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HEV2583033.1"
                     /translation="GIGTFGLDRHGKNRIQWEDYLIGLLLANPELSSEVCAIITDGDF
                     AGTDTRELYRIFNSVNQRGASPLFQSLEQAVPSALLSTLERARKSTELRSSLEGAGLI
                     KAAVQCATRLKKASLSQQNTELQYLMKEAGEAGDAEAVRYYRQQLSALHQQRRTIDLA
                     THLQG"
BASE COUNT          957 a         1329 c         1193 g         1037 t
ORIGIN      
        1 ttattttttc agcgaaggat tgcagcgatt cattttgcat agtcggtaac ataggtaaag
       61 cgtcttcccg aataaagcgt aaacacattt tctcatttcc agtatagcag gggcttagga
      121 attttctgtt cgcgtcaaac atcgaaaagg aaaaaaaatg gagatcggtt tctttttgcg
      181 cagcctgttg attggcctgt cgattgccgc cactgtcggc cctatgagca tcctgtgcat
      241 tcaacgcact ctgcaaaaag gctacgttta tggcctggtg tccggcctgg gagtcgcgac
      301 ggctgatggt tgctacggca gcgtggccgc ctttggcctg acgatcatct cgaccttcct
      361 ggtcagccaa caaagctgga ttcggctgat cggcggcctg ttcctcgtct acctcggcac
      421 aaggacaatc ctgagccgtc cgccagaacg cgccgccgta ccgaaagccg gagtaggcac
      481 cttgctcggc gcctatacgt ctaccttcct gctgaccctc accaacccgc tcacgatcct
      541 ctccttcgcg gccatcttcg ccggcctggg agtcgcgagt acaggccgca attacctggc
      601 agcaacgctg gtggtggccg gggtgttctg cggctctgca ctgtggtggt gtatcctgac
      661 aggaggtatc agcctgctgc gcgcgaggtt cacgacgaca tggatgctct ggctcaatcg
      721 cctttcggga tgcatcatcg cggcctttgg gattatcgcg ctggttagtc tcatatgatc
      781 gttgtgatgc tattggtcgc ttgctcgacg tacctcaacc tgcccattat tcacgcgcac
      841 ctcgtaacgc ggctcgcgcg cggtggccgg gccagccagc acacgaccgt cgcgtatgct
      901 gaagcgcgag ccatgccagg gacactgcac cacgtcccct tcaagcgtac cttcatccaa
      961 tggtccacca gcatgagtgc aggttgcgga taaggcgaat acttgcgaat cgtgacgcac
     1021 tagcacaacc ggaacgccgg aagccatgac acgaaagagc ttgccttcct caacctgctc
     1081 aagcggcatg atggcctcaa aggtgtcgct tcctgtttcc catgccgtat ggttgacgtt
     1141 cgtgcctttc gtgtaaacca tatcgccgcc aagataggct gcgtaggcta ccaggatgaa
     1201 gccagcaaaa ccgaagatcg cccctgatat gctatcgcca gccggttgaa tcaatcgcaa
     1261 caccagcgag ataatataga gaacgagtgc cgatacatta aaaatggcat ggttcagacc
     1321 aacccggcgt tcagcgccat aggtatcgct ccaatccgtc aagccggttg cagcagctcc
     1381 cagcgcgccg agaataccga caattaccgc ggcctcagcg ccacgcgcag cccaggcgtt
     1441 agaagaaacc agccaggtca cgtcaaaaac tactgtcaaa agccaggcac cgatcggaac
     1501 gtcggtcagc gcgggatgta ggggatgtcc taaccagctt ccattgagga aactcttgaa
     1561 gcggcgcggt ggcttgcgat tcgcgccaac cagcacccca atgatctgct gcaaacgatc
     1621 gctcaaccgt tccgcgaatg gctgaggcgc tgactcagat gtcgttgttt cggtggacgt
     1681 ttcactcgcc gcccgagaga tgatgccgcc ctgacaacca caggtttcac acatgtgatg
     1741 ctgctccttt cattttgcca tgaaggtcat acttcttcat ataacacgaa aagcagagaa
     1801 agtcaattat tttcttgact ttattgacaa acgacaacgg aattggtaca ctgaaagctg
     1861 agtaaaagga ggagggcgca tattgaactg gtatcgtcgc ttttttttga gtacgcgggg
     1921 gcgcatcctg tcattgattc gacgcgagag tcgcacagtg gatgatctag ccgacgcact
     1981 tggcctgacg gacaatgcgg tgcgcgctca tctcgccgcc ctggaacgcg atggcttcgt
     2041 gcaacagcgc ggagcgcgta agggaagcag caaacctgcc tttgtttacg aactggcgga
     2101 gggagcagaa ccgcttttcc ccaaggcgta tggacagata ttgaacgaac tgttggcagt
     2161 gcttgataag caattgaatg cagaagagct ggaggaactc ctgcgcgcca ccggcagaca
     2221 cattgcggcc aggtggaaca ggctgtcagg cagcacttat gcgcgtcttg aagcagccgt
     2281 tgagatactc aatgaattag gcggacttgc cgatctggag ccgctcaatg gcggctattg
     2341 catccgcagc aatagctgtc ctctcgccgc agtggtaccc gaccaccctg ccgcctgtca
     2401 tctcgccgaa acactgatag ccgagctggt tggcgtgcca gttcaggagc gctgcgaaaa
     2461 aggtgattct ccgcgctgcc gttttgaagt cacgccgata taagcaagaa gaaggggagg
     2521 gcaggccaat ggcctgccct ccccttcttc ttgcttacta aacaatctac taatccaggt
     2581 agtccttcag cttgcggctg cggctgggat ggcgcagttt gcgcagggcc ttcgcctcga
     2641 tctggcgaat gcgctcgcgc gtgacgtgga actctttgcc cacctcttcc agcgtgcggc
     2701 tgcggccatc gtcgagaccg aagcgcagct gcaagacctt gcgctcccgc tcggtcaggc
     2761 tggccagcac atcctcgacc tgctccttga gcagttgatg gctggcggca tcggcagggg
     2821 ccagcgcggt gtgatcttcg atgaagtcac ccaggtggct gtcctcttcc tcgccaatcg
     2881 gcgtctccag gctcaccggc tcctggctga ccttgatgat ctcgcgcacc ttctccgggc
     2941 tgatctccat ttgctcggcg atctcttcgg aggtcggctc gcggcccagg tcttgcagca
     3001 gtcggcggct gatgcggatc aggcggttga tcgtctccac catgtgaacg gggatacgga
     3061 tcgtgcgagc ctggtcagcg atggcgcgcg taatggcctg gcgaatccac cacgtcgcat
     3121 acgtgctgaa tttgaagccc ttcacgtaat cgaacttctc gacggcacga atgaggccga
     3181 tgttgccttc ctggatcagg tccagcaggc tcataccccg gccaatatac tttttagcta
     3241 cgctgacgac caggcgcaag ttggcctcgg tcaaacggcg ctgcgcctcc tcgccatctt
     3301 cgatcaagcg gtaatcgcgc atcagggcgg gcttgcgcag ttcggttctg ccgcgctcca
     3361 tgcggcgcgc caggcgcact tcttcctcgc cggtgagcag gggaacacgc ccgatctcgc
     3421 gcaggtacat gcgcacggga tcgtccagtg tgctttccag atcggccatc acaatgccga
     3481 ggccatcatg caactcatcc catttatcct gggcttccgc ctccacgtcg atctcatcct
     3541 ccggcccagg ctcccagtca acgggtggct ctttgtttgg gtcaagggta tccatagatt
     3601 catcgtcctg caacttgaac aggtcaaacg actcagtcgt atcgacagga gtcacaaagc
     3661 tgcctcccgt tgcggcgtga gtatgaccgc tgagcgtgtt catatcaaaa tcggcgttcc
     3721 catccaggat ggaaacctca aacgctccct ctacaaaatg caacggttcg gtgttaactt
     3781 tttctaacag cacttcttca ttcaccattt cggcatcccc cttcagcaaa caggtctctc
     3841 agaataagaa gaacgatgat agctcctgtt tcctgactct ggttcctaca attgtttccc
     3901 tcgcggcccc ggcgcggttc ccggcatcgt tcaggactct acaaaaagac aatcattaag
     3961 aataattgcg ccaatgttgt ttcactcatc catccacttc gtccaatcac tcactctcta
     4021 tccctgcaag tgcgtcgcca aatcaattgt gcgcctctgt tgatgtaagg ccgacaattg
     4081 ctgccggtaa taccgcaccg cctcggcatc tccggcctca cctgcttctt tcatcaggta
     4141 ttgtaactcc gtattctgtt gtgacaggct ggcctttttg agccgcgtcg cacactggac
     4201 tgctgctttg atcagccctg ctccttctaa tgaagatcgc aactcagtgc ttttccgcgc
     4261 gcgttcaagc gttgacagca gcgcggaagg cacagcctgc tctaaggatt gaaataaggg
     4321 ggaggcacca cgttgattca ccgagttgaa tatgcggtac aactctcgcg tgtctgtccc
     4381 tgcaaagtca ccatcggtta ttatagcaca aacttcggaa cttagctcag gattcgccaa
     4441 gagtagccca attaggtagt cttcccattg tatcctattt ttaccatgtc tgtcaagtcc
     4501 aaatgttcca atcccg
//
LOCUS       DASZAK010001097         2435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_28184, whole genome shotgun sequence.
ACCESSION   DASZAK010001097 DASZAK010000000
VERSION     DASZAK010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28184"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1034)
                     /locus_tag="VGT44_19395"
     CDS             complement(<1..1034)
                     /locus_tag="VGT44_19395"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2583034.1"
                     /translation="MKPTATDQKLRGGYYTPKPIATFLANWAIQTPDTSILEPSCGDG
                     MLLEAAAKRLTECGATDTSIHTSIQGIEFDGFEAQKAVERLRAIYDSYAPQIMTGDFF
                     TYCQKYLNQERRFDAIIGNPPFIRYQHFPENQRAIAFYLMQRAGLHPNRLTNACVPFL
                     VASTSLLSKTGKLAMVIPAELLQVNYAAELRYFLSQHYSYITLITFKKLVFDGIQQEV
                     VLLLAERNGSEHTGIRTIELDGIDDLLAYEHTVLSKDKLKVMDHSTEKWTMYFLEEAE
                     KLPLPLIGADTLDIQQLDALLHDGNIYAALDITDHVLLREGLGLSSKDTAMLRTIWQK
                     LRDRRINRKL"
     gene            complement(1031..2245)
                     /locus_tag="VGT44_19400"
     CDS             complement(1031..2245)
                     /locus_tag="VGT44_19400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HEV2583035.1"
                     /translation="MPSEVIELRGHIIDSWILPRAWDTIMDRGGNFEVEEMQVGKHKT
                     ETSYVRLKIEAPDDETLELILSEVQQLGGVLLHGNDVQTARVEQDGVLPAKFYSTTNL
                     PTQVRLNGQWVEVEQIEMDVAIVIDRAARRAYGKPMHDVVIGEEVVIGHEGLRVTPFE
                     RAREREVFAFMQSDISSEKAKVQTIHRIAHEMQETRARGGKILFVLGPAVIHTGAGRY
                     VADMIRRGYVNVIFGGNAIITHDIESALFGTSLGVDLQSGEQVEGGHRNHLRAINAIR
                     ALGTVEHAIETGLLHEGITYEATRHDVKMVLAGSVRDDGPMPGVINDMQQAQRAMRAA
                     LPGVEMAIIVASMLHAIATGNLLPAAVRTVVVDMNPSVVTKLADRGSFQAVGLITDAE
                     LFLRELAEALSS"
BASE COUNT          472 a          628 c          724 g          611 t
ORIGIN      
        1 tcaagctttc tgttgatacg ccggtcgcgc aacttttgcc agattgtgcg caacatagct
       61 gtatctttgc tgcttaatcc taacccctcg cgtaaaagaa catggtcggt aatgtcgagc
      121 gccgcgtaga tattcccgtc atggagaagt gcatcgagct gttgtatgtc aagcgtgtct
      181 gctccaatta aaggcaaggg cagtttttcc gcttcttcaa gaaaatacat cgtccacttt
      241 tctgtagagt gatccatcac tttcagcttg tcttttgaca agaccgtgtg ttcataagca
      301 aggagatcgt caatgccatc aagctcaata gttctaatgc ctgtatgttc gctgccattt
      361 cgctctgcca ggagtaggac gacttcctgt tggataccgt caaagacaag tttcttgaag
      421 gtaatgagtg taatatagct gtagtgctgg ctgaggaagt agcgcagttc tgccgcgtag
      481 ttgacctgga gtaactcagc cgggatgacc atggctaact tgccagtctt gcttagcagt
      541 gaggtagatg caacgaggaa tggaacgcag gcattcgtta gtcggttggg atgtaagccc
      601 gcgcgctgca tgaggtagaa ggcgatggcg cgttgatttt ctggaaaatg ttgatagcga
      661 ataaaaggag gattaccaat gatggcatca aatcgccttt cttgattgag gtacttctgg
      721 cagtaggtga agaagtcgcc tgtcattatc tggggagcgt atgaatcata tatggcgcgt
      781 aggcgctcaa cagccttctg tgcttcaaag ccatcgaatt cgatgccctg aatagaggta
      841 tggatagaag tatccgttgc gccgcactct gttagccttt tagcggcagc ctcaagcagc
      901 atcccatctc cgcagcttgg ctccaagatg cttgtgtcag gggtctggat ggcccaattt
      961 gcgagaaaag tagcaattgg ttttggggtg taatatcccc ctcgcaattt ttgatctgtt
     1021 gctgtgggtt tcatgaagac agcgcctcgg ccagttcccg caggaacagt tcggcatcgg
     1081 taatcaggcc gacggcctgg aagctgcccc ggtcggccag tttggtgacg acggaggggt
     1141 tcatatcgac gacgacggtg cgcacggctg ccgggagcag gttgccggtg gcgatggcgt
     1201 gcagcatgga ggcgacgatg atggccattt ctacgccggg aagggcggct cgcatggctc
     1261 gctgcgcctg ctgcatgtcg ttgatgacgc cgggcatggg gccgtcgtcg cgcacggaac
     1321 cggccagcac catcttcaca tcatgcctgg tggcttcgta ggtgatgccc tcgtggagca
     1381 ggccggtttc gatggcgtgt tccaccgtgc cgagtgcgcg aatggcgttg atggcgcgca
     1441 ggtggttgcg atggccaccc tcgacctgct cgccgctttg cagatcgacg cccagcgaag
     1501 tgccgaagag cgccgattcg atgtcgtggg tgatgatggc gttgccgccg aagatgacat
     1561 tgacgtagcc gcgccggatc atgtcggcga cgtaacggcc agcgccggtg tggatgaccg
     1621 ccgggccaag cacgaagagg attttgccgc cgcgcgcgcg cgtctcttgc atttcgtgtg
     1681 cgatgcgatg gattgtctgg actttggctt tctcggaaga aatatcgctt tgcatgaagg
     1741 cgaagacctc gcgttccctg gcgcgctcga agggcgtcac gcgcagtccc tcgtggccga
     1801 tcaccacttc ttcgccgatc accacatcgt gcatcggctt gccataggcg cgcctggcgg
     1861 cgcggtcgat cacaatcgcc acatccatct cgatctgttc cacctcaacc cactgcccgt
     1921 tcaggcgcac ctgggttggt agattggtcg tagaataaaa tttggcgggc agcacgccat
     1981 cttgctcgac cctggcggtc tgtacgtcgt tgccatgcag caacacgccg ccgagctgtt
     2041 gcacttcgga gaggatcagt tccagcgtct catcgtcggg cgcttcgatt ttcaagcgca
     2101 catagcttgt ctcggttttg tgtttgccga cctgcatctc ttcgacctcg aagttgccgc
     2161 cccggtccat gatggtgtcc caggcgcgcg gcagaatcca ggaatcgatg atgtgacctc
     2221 gtagttctat gacttcgctt ggcatggctg tttcaactct ctttcgttgc gcttatttat
     2281 tcttgccaat gacgcagagg ggtttgaaat cgcattggcg gcatttggct gctttctggc
     2341 ttgccagggg cggcggctgt tgctggctgc atttctgcat ggttgccagc aggcgaatga
     2401 ccttcttttt gagcgcgggg gtataatcga cggta
//
LOCUS       DASZAK010001098         2286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_526639, whole genome shotgun sequence.
ACCESSION   DASZAK010001098 DASZAK010000000
VERSION     DASZAK010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526639"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VGT44_19405"
     CDS             complement(<1..349)
                     /locus_tag="VGT44_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583036.1"
                     /translation="MVVARRTRMPAPPAGQGAPIIHGNPVLHKWLTHYWQKLELPARE
                     LGLLAVTQDRQEYARWTGKRLNIMALGCYCYLPAPSAARARTRKRAPSPSLPGFGDTT
                     RVAQAAAHRHLIFI"
     gene            complement(452..1606)
                     /locus_tag="VGT44_19410"
     CDS             complement(452..1606)
                     /locus_tag="VGT44_19410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583037.1"
                     /translation="MRIYRLNIMFPLLLLALLLAACSGGNSNAPAQATPKPSPAPEQG
                     AQLLAKAGTALNGAKTLHALLDIGITGSGFNGTVNAEVWSESPGKNRTVVLQSTLAQL
                     SSGEITVSNGQQVWQYDPAKKVVYTAKLNGTNTSTGQDTSQFLFSLVRSIFSSSQATL
                     VTSSASVKGHAVYDVHVAQSMKTNGSGGTSATPGAGNADLNYIGDVYLDKTTYLPVEV
                     VLTIQGFGQATIDFPTLQLNQPVSEQLFTFAPPAGVKVLPFPQSTGDTGSLTLAQAQQ
                     QAGYHLLSIPASQAAYAMQSVNALGAPGNQIYTLNYLANGSTPFTISEGKALANLPAS
                     GQPVKVRGANGAIATVGSIATLSWTENGVGIQVAGALSSAQLMTVAGLLA"
     gene            complement(1768..>2286)
                     /locus_tag="VGT44_19415"
     CDS             complement(1768..>2286)
                     /locus_tag="VGT44_19415"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2583038.1"
                     /translation="REVGYRRGEGIALNNLGGVYDDLGQKQKALGYYEQALAIYREVG
                     DRGGEGATLNNLGEFNRTSGQKQEALGYYEQALAIRREVGDRGGEGITLHNIGTIYRE
                     QENYDKALACFLLARGIFEELQSPHRDDEQGWIDGLRKKVGEEQYSALLAQVEPRARE
                     IVEQALREGGML"
BASE COUNT          421 a          716 c          664 g          485 t
ORIGIN      
        1 cgatgaagat caggtggcga tgtgcggcgg cttgggctac gcgggtcgtg tcgccaaatc
       61 cgggcagcga tggagatgga gcgcgtttgc gtgtacgcgc acgcgccgcg gaaggggcgg
      121 gaaggtagca atagcagcct agcgccatga tgttgagacg cttgcccgtc cagcgcgcat
      181 attcctggcg atcctgtgtg accgccagca gtcccagctc gcgcgctgga agttctaatt
      241 tctgccagta gtgggtcagc cacttatgta aaacgggatt cccgtgaata atcggcgcgc
      301 cctgccccgc tggaggagca ggcatcctgg ttcgacgcgc gacaaccatg tccgcagccc
      361 ttctcctgat ccctgatcgt acatatcgta cataacgcac gttttgcgca tcgccagtat
      421 agcaggcacg gacgagagat gcaagagccg attacgccag cagccccgcg accgtcatca
      481 attgcgcgct gctcaatgcc ccggcgacct ggatgcccac gccgttttcc gtccacgaga
      541 gcgtggcgat gctgccgacc gtcgcgattg cgccattcgc gccgcgcacc ttgaccggct
      601 ggccggaggc gggcagatta gccagcgcct tgccttcgga gatggtgaac ggcgtgctgc
      661 cgttggcgag gtagttcagc gtgtaaatct ggttgcccgg cgcgcccagg gcattcacgg
      721 attgcatggc ataggccgcc tgggaggcag gaatgctgag gaggtgatag cctgcctgct
      781 gctgcgcctg cgccagcgta agcgagccgg tatcgcccgt actttgcggg aagggcagca
      841 ccttgacgcc cgcgggaggc gcaaaggtga aaagctgctc gctgaccggc tggttgagtt
      901 gcagcgtcgg gaaatcaatc gtagcctgcc cgaagccctg gatcgtcagc acgacctcga
      961 cgggcaggta ggtggtcttg tccaggtaga catcgccgat atagtttaaa tcggcattgc
     1021 ccgcgccggg tgtggcgctt gttccgcccg atccattcgt cttcatgctc tgcgccacat
     1081 gcacatcgta gacagcatga cccttgacgc tggcggagga agtcaccagc gtggcctggc
     1141 tgctgctaaa gatggagcgc acgaggctaa acaaaaactg gctggtatcc tgtcccgtac
     1201 tggtattggt gccattcagc ttcgcggtat acaccacctt cttcgccggg tcgtattgcc
     1261 agacctgctg gccgttgctc accgtaatct ccccgctgga caactgcgcc agcgtcgatt
     1321 gcagcacaac cgtgcggttc ttacccggcg actcgctcca gacctcggca ttgaccgtgc
     1381 cgttgaaccc cgacccggta atgccgatat cgagcagcgc gtgcagcgtt ttagccccat
     1441 tcaacgcggt tccggccttc gccagcaact gcgccccctg ctccggcgcg ggcgacggct
     1501 tcggcgtagc ctgcgcgggc gcgttgctgt taccgccgct gcacgccgcc agcagcagcg
     1561 cgagcaggag gagaggaaac atgatattca ggcgatatat gcgcatggat tactccttat
     1621 tcaaggtttg tgaatgattg ctgaaagtgt gaggcaggag gtcagcaggg cgaccacaag
     1681 ggtacgccct gctgaccgtt ccatatatca ttcatatgcc ctggttccat ttcgtgtcat
     1741 tagacaattg acgatccact acccatttta caacatcccg ccttccctca acgcctgctc
     1801 cactatctcc cgcgctcgtg gctcaacctg ggccaacaac gcgctgtatt gctcttctcc
     1861 gacctttttg cgcaggccat cgatccatcc ttgttcatcg tcgcgatgcg gactttgtaa
     1921 ctcctcgaag atgcccctgg cgagcagaaa acaggcgaga gccttatcat aattttcttg
     1981 ttcacgataa attgttccaa tattatgcag agttatccct tcgccgcccc ggtcgcccac
     2041 ctcgcgtcgg atggccagcg cttgctcgta gtagcccagc gcctcctgct tctgccccga
     2101 agtacgattg aattcaccca ggttgttgag ggtcgcgcct tcgccgcccc ggtcgcccac
     2161 ctcgcggtag atggccagcg cttgctcgta gtagcccagc gctttctgct tctgccccag
     2221 atcatcatag accccgccca ggttgttgag agctatgcct tcaccgcggc gatagcccac
     2281 ctcgcg
//
LOCUS       DASZAK010001099         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_28242, whole genome shotgun sequence.
ACCESSION   DASZAK010001099 DASZAK010000000
VERSION     DASZAK010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28242"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..1478)
                     /locus_tag="VGT44_19420"
     CDS             complement(15..1478)
                     /locus_tag="VGT44_19420"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV2583039.1"
                     /translation="MRISSPIWTRLKWPGRVGLVLAPTLLLTVLLAAHVVPLPLPAKT
                     VVFGTPVIPNAFRQPLQLSSDPYKDGSSQPQSEVEPGAFAAGDTIVTAFQAGRYQDGG
                     SANNGWATSKDDGHSWKSGFLPGTTQFAGGPYTRLSDPSVAYDAAHNIWLIASLAIVG
                     SGSTLATHTIIVNISTDGGFTWSKPYKVVDGGSTYYDKDWITCDSTPSSPFYGHCYVE
                     WDNNDLGGLILVSTSSDGGHTWSKPQTTADHALGIGGQPLVQTNGTVVVPISSIDGTH
                     LLSFLSTNGGKSWSKTTIIAPVNGYVLATAAIDGANTIYVVWADCRFEKQCVSQGGSS
                     DTTPPAGQQDDLVMSTSSDGRHWSAPRLIPADPLGSGVEHTITGLGVDPNTWGSSAHL
                     ALTFYYHSINCPSDCPYSVGFTSSEDGGKHWTPKIQIAGPMNTSWLPTGRNKVGDYIS
                     TVICNGLAFPFFSSADQPDANGPLSETIDTITGGLNV"
     gene            1657..>2306
                     /locus_tag="VGT44_19425"
     CDS             1657..>2306
                     /locus_tag="VGT44_19425"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEV2583040.1"
                     /translation="MTVHVRTLGAGKQRIVEAVRAGGLDGMLLCSPEHVYYTTGLPAV
                     QGSGNPILFALRNQLPYFVYVGADGTMTLLCWIGATMGFSFDVDEVRSFFSTSSALDE
                     LRDFLKATLRPGGKVGIESSCPWNVVTLMQKVAPSTELSEIDELMLGLRMVKTTEELE
                     CIRRATTIVEATVTDLRASVRPGISRVWLIGEAKRLMLAHGASGIGHTTIAFGSSNA"
BASE COUNT          466 a          622 c          712 g          506 t
ORIGIN      
        1 cggactacaa gagtttaaac atttagcccg ccggtaatcg tatcgattgt ctcgcttaat
       61 gggccgtttg cgtcgggttg gtcagcgcta gagaaaaacg ggaaggccag accgttgcag
      121 atcacagtcg agatgtagtc accgactttg ttcctgccgg tgggaagcca ggaggtattc
      181 attggcccgg caatctggat ttttggcgtc cagtgcttcc ctccgtcctc ggatgacgtg
      241 aagccaaccg agtaggggca atcgctgggg cagttgatgg agtggtagta gaaggtcagg
      301 gccaggtgtg cgctacttcc ccaggtgttc gggtctacgc ccaggccggt gatggtgtgt
      361 tcgactccac tgcccaacgg gtcagccggg atgagacgcg gcgcagacca atgtctacca
      421 tcgctgctgg tagacatcac caggtcatct tgctgaccgg caggcggcgt tgtatcgctg
      481 gagccgccct ggctcacgca ttgcttctcg aagcggcaat cagcccacac aacatagatg
      541 gtatttgcgc catcgatagc tgccgttgcc agcacatagc cgttcaccgg ggcaatgatg
      601 gttgtcttgc tccaactttt gccgccattc gttgaaagaa acgagagcag gtgcgtacca
      661 tcgatagagc tgatcggaac aacgacggtg ccgttcgtct gcacgagtgg ctggccgccg
      721 atacccaggg catggtcagc cgtcgtctgc ggtttgctcc aggtatgccc gccatcgctg
      781 gatgtactca cgaggatgag gccgcccaaa tcgttgttgt cccactcgac gtagcagtga
      841 ccatagaagg ggctggatgg cgttgagtcg caggtaatcc aatccttgtc gtagtaggtg
      901 ctgccaccat ccacgacttt gtacggcttg ctccaggtaa agccgccatc agttgagata
      961 ttaacgataa tggtatgcgt ggccagggtt gacccgctgc ccacaatcgc cagtgatgca
     1021 atcagccaga tgttatgcgc ggcatcatag gccaccgatg gatcgctcag gcgcgtgtag
     1081 ggcccgcctg cgaattgcgt cgtgccgggc aagaagccgc tcttccagct atgcccatcg
     1141 tccttcgagg tggcccagcc gttgttggcg ctgcctccgt cctggtagcg tcccgcctga
     1201 aacgccgtca cgatcgtatc gccagcggcg aatgcacccg gctcgacctc cgattgaggc
     1261 tggctgctgc catccttata ggggtcactg ctcaattgca gcggctgccg gaaagcgttg
     1321 gggatgaccg gtgtgccaaa gaccaccgtc tttgcaggca gcggaagagg aacaacatgc
     1381 gccgccagca agacggtgag cagcagcgtg ggcgccagca cgaggccaac acgccccggc
     1441 cacttcaagc gagtccagat tggtgatgaa atgcgcatag cgttgtccac cggtaattgg
     1501 gactataacc tacatatata ttctacgcta tattaacgcg actggtgggt aattcttgag
     1561 attcggttgt actggtaaaa ccgctccatt cctggtatgc ttgatatggt tgagcatggt
     1621 gccacaattc ttgaggaagg aagtcaactc accacaatga ctgtgcatgt gcgaacgctc
     1681 ggagcgggaa agcagcgtat cgtggaggcg gtgcgcgccg gtggactgga tggcatgttg
     1741 ctttgttcgc cggagcatgt gtattacacg actggcttgc ccgccgtgca gggtagcggc
     1801 aacccgattc tattcgccct ccgcaatcag ctcccctact ttgtttacgt cggcgcggat
     1861 ggcacgatga ccctgctgtg ctggatcggc gcgacgatgg gattttcgtt cgatgtcgat
     1921 gaggtgcgca gtttttttag cacgagcagc gcgctcgacg aactgcgcga ttttctgaaa
     1981 gctacgctgc gcccaggggg caaggttggt attgagagta gctgcccctg gaatgtcgtc
     2041 acgcttatgc aaaaggttgc gccttcaaca gagttgagcg aaatcgacga gttgatgctc
     2101 ggcctgcgta tggtcaaaac gaccgaggaa ctggagtgca tacgcagggc gacgacgatt
     2161 gtcgaggcga cagtgactga cctgcgggcg agtgtcaggc cgggtatttc gcgcgtgtgg
     2221 ctgatcggcg aggcgaaacg gctcatgctg gcgcatggcg cgagcgggat cgggcatacc
     2281 actatcgcgt ttggctcctc caatgc
//
LOCUS       DASZAK010001100         5280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_384559, whole genome shotgun sequence.
ACCESSION   DASZAK010001100 DASZAK010000000
VERSION     DASZAK010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384559"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..588)
                     /locus_tag="VGT44_19430"
     CDS             complement(4..588)
                     /locus_tag="VGT44_19430"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEV2583041.1"
                     /translation="MKKLIVTSLLVLLIVLSVAIYNYSRRVTGTILPGMTPAGWQPAQ
                     LQIPALNINAPVMGVGQAADGTMDAPVSNQYNSPYWTNVFWYDLGAAPGQPGNVVIAG
                     HVDRVGGDPAVFWSLGRLQPGDTITVVTQDGTTYHYRVDRTVRYPANFPGQQAINDVF
                     GPTNAHHLNLITCSGVWTGNGYDERLVVFTSQVG"
     gene            complement(629..1531)
                     /locus_tag="VGT44_19435"
     CDS             complement(629..1531)
                     /locus_tag="VGT44_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2583042.1"
                     /translation="MQLSIKHLTKVYKGDVRALDGIDLTIPGGMYGLLGPNGAGKTTL
                     MRILAGILRPTGGDVLIGEHDGNTEKGRTAVKRVLGYLPQELGVYPDLTAREFLDYVG
                     ILKGLDDRRARQHRVGELLELVSLSQVANRKLKAFSGGMKRRIGIAQALLNDPRLLIV
                     DEPTAGLDPEERIRFRNLLSELGGDRTVLLSTHIVEDVAQTCRNLAIMQNGRIVFQGT
                     TLDLLGEARGKVWIVSTGGTKPQGDLTIVSTLHMGDSVQYRLVGALDEPEQYDASPAE
                     PSLEDSYVWLMRAQRTSEKMTTAK"
     gene            complement(1578..2408)
                     /locus_tag="VGT44_19440"
     CDS             complement(1578..2408)
                     /locus_tag="VGT44_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583043.1"
                     /translation="MMQVGQTEIMRHASRAINQRTIVWGVLRYEFLMQIRRRVLWLAY
                     GGFSLLLFNTALGVLHNPEQPVLSLPLMQMVASVTVQLNWLPVIGIGIFLADRLPRDR
                     ANKVDELFEAMPGTLRSRLAGKYFGCVLASLIPAFLTYIIFIGVLAVYKHTLLVFPVA
                     LLTYSVIVLPGILFVAAFTLACTAVLWVPLYQFLFVGYWFWGNMLSPHIGLPTLSGTI
                     LTPIGSVICAGIFGVVPYKYMQNATPLEGATSLFVLLAIPLLVMLLFYYLLKWEQARK
                     "
     gene            complement(2405..3190)
                     /locus_tag="VGT44_19445"
     CDS             complement(2405..3190)
                     /locus_tag="VGT44_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583044.1"
                     /translation="MNEQLKAFSGVVKYEFLMQIRRPVLWIAYLCLSLLFVRLGRGGP
                     FYTLIFNPYNQSIMALSAEWTRNLMFVFPIVYGVLLADRLARDRRRKVEEILLTLPSA
                     TTTRMLGKYVGSSLAALLPLFLYDCAGIGFLLFSSHNLMALPDGLLKFAVVALPGLLF
                     VSAFSIACPSIMWVPLYQVLFTGYWFWGNILGPSSLIPSLSTTILTPVGGYMASGFFG
                     DNLQLIEHATAVQGMESILLLLGITALVMYSLCKVVAWQQERQ"
     gene            complement(3187..3924)
                     /locus_tag="VGT44_19450"
     CDS             complement(3187..3924)
                     /locus_tag="VGT44_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583045.1"
                     /translation="MTLERLYYELRLMGKRVILTPVLLATGFALFAAFLATRQIDPAR
                     FLSGGLEMLLPLATGMIVATIASHDPALELQLTVPRAYAATAVKRMALVAGWAIAIAL
                     LASALLSALRLTYVPQQLNSWPTALAFLTNQLTWLAPLCWMVAIGLCLALLLRSRTAS
                     SAIVGGIWILEIIFKDGLAAIPWLHPVLLFPTTLILFPSPFISNDVFWTWLTSRCEIL
                     GTGLLLLVLAWLLLRNPEGLLKGASEE"
     gene            complement(3937..4932)
                     /locus_tag="VGT44_19455"
     CDS             complement(3937..4932)
                     /locus_tag="VGT44_19455"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEV2583046.1"
                     /translation="MNEHSLPRQQASHDSVQKNRLTRTGHISDLLPAYLNGRLDAHSG
                     AEVRAHLALCQTCEAELASWQAISKATHAEEEALPLPAAHTMSRVWEKLDAMEIQQAQ
                     APRHTLQEIAQHMWLVFTRQIPLIHKSIWIASVLVNILMLVLVFLSDPFSIHAHERSI
                     EGVLALFTTVVAAAGIAFIYQAEKDAGYEVILSTPTSIRVMMICRMILVIGHNFLLAA
                     ITSGIIASITGGNVWDFAQLWFGPMLLLASISLSFSVMLGSAVGIAASLVIEVLQAMA
                     TSVEKVVPALQFLRDTIGQTTPWTVLAALALLAFAIYYAPRQPRLSGASSTYNQR"
     gene            complement(4929..>5280)
                     /locus_tag="VGT44_19460"
     CDS             complement(4929..>5280)
                     /locus_tag="VGT44_19460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2583047.1"
                     /translation="SVRTWLIGIARRQAHNTLRQRKLLLVDESEMAEMAASDPEPEAL
                     TLASIACDELTELFQRLAPYHREVLALIFVQELSYQETATILDVPLGTVKSRLNNARR
                     ALRALLDAREEAHR"
BASE COUNT         1176 a         1501 c         1572 g         1031 t
ORIGIN      
        1 gaatcagccc acctgtgagg taaacacgac cagccgctcg tcgtagccgt taccggtcca
       61 gacgccgctg caggtaatca ggttgaggtg atgcgcgttg gtcgggccaa aaacgtcgtt
      121 gatagcctgc tggccgggga agttagcggg atatctgacc gttcgatcaa cccggtagtg
      181 ataggtggtg ccatcctgcg tgacaaccgt gatggtatcg ccgggttgca gcctgcccag
      241 cgaccagaac acagcagggt caccgcccac gcgatcaacg tgcccggcga tgaccacatt
      301 gcccggctgt cccggcgccg ctcccaggtc gtaccagaag acgttcgtcc agtagggcga
      361 attatactgg ttggagaccg gtgcatccat tgtgccatct gccgcctgtc ctacacccat
      421 caccggcgcg ttgatgttca gcgcgggaat ctgcaactgc gcgggctgcc agcctgcggg
      481 cgtcatacct ggcaggattg tgcctgtgac gcgcctggaa taattgtaga tagccacgct
      541 cagcacgatc aacaggacaa gtaaggatgt aacgatcagc tttttcattc gatgggcaat
      601 tccctcacct gcatggtgac aggagaactc attttgcagt agtcattttc tcggatgtgc
      661 gctgcgcgcg cataagccag acgtagctat cttccaggct cggctctgcc ggggatgcgt
      721 cgtactgctc tggctcgtcc agcgcgccga ccagccgata ttgcaccgag tcacccatgt
      781 gcagggtcga gacgatagtg agatcgccct gcggcttcgt gccgccggta gatactatcc
      841 atactttacc acgcgcctca cctagcaggt caagcgtggt accctgaaag acaatgcgcc
      901 cattctgcat gatggcgagg ttgcgacagg tctgcgccac atcctcgacg atatgcgtcg
      961 agagcagtac cgtgcggtct ccgcccaact ctgagagcag gttgcggaaa cggatgcgtt
     1021 cctccgggtc aagccccgcc gtcggctcgt caacaatcag cagcctgggg tcgttgagca
     1081 gggcctgcgc gatgccgatg cgccgtttca tgccgcccga aaacgctttt agcttgcgat
     1141 tggcgacctg gcttaacgac accagctcca gcaactcgcc gacgcggtgc tggcgcgccc
     1201 tgcgatcatc cagccccttg aggatgccga cataatcgag gaactcgcgc gcggtcaggt
     1261 cgggatagac gcccagctcc tgcggcagat agcccagcac gcgcttgacc gccgtgcgcc
     1321 ccttctcggt attgccgtcg tgttccccga tcaggacatc cccgccggtt ggccgcagaa
     1381 tgcccgccag aatgcgcatc aacgtcgtct tgcccgcgcc gttcggcccc agcaacccgt
     1441 acatgccgcc aggaatcgtc agatcgatgc cgtccagcgc gcgtacatca cctttataga
     1501 cctttgtaag atgtttgatt gatagttgca ttacataatc ctcctctatt gctctgattg
     1561 tagggacagg gggcttctca ttttctcgct tgctcccatt ttagcaaata ataaaaaagc
     1621 agcatgacca gcaggggaat ggcgagcagg acgaacaggc tggttgcgcc ttccagcggc
     1681 gtcgcgtttt gcatatattt ataagggacc acgccgaaga tgcctgcgca gatgacgctg
     1741 ccaatcggag tcaggatggt gcctgaaagc gttgggaggc cgatatgcgg gctgagcata
     1801 ttgccccaga accagtaacc cacgaaaaga aactggtaga gcggtaccca taacaccgct
     1861 gtgcaggcca gcgtaaaggc ggcgacgaag aggatgcccg gcagcacgat cacgctgtag
     1921 gtcagcagcg caaccgggaa aacgagcaac gtatgcttgt agacagccag gacgccgatg
     1981 aaaatgatgt aggtgagaaa tgccgggatg agggaggcca gcacgcagcc gaaatatttg
     2041 cccgcgagtc tcgagcgcag cgtccctggc atggcttcga acagttcatc aactttgtta
     2101 gccctgtcgc gtggtaagcg gtctgccagg aagatgccga tgccgataac cggcagccag
     2161 ttcaactgca cagtgaccga tgctaccatt tgcatgaggg gtagcgacaa cacaggctgt
     2221 tcgggattat gcaaaacgcc gagggcggtg ttgaacagca gcagggaaaa gccgccgtag
     2281 gcgagccaca ggacgcggcg gcgaatctgc atcaggaatt cgtagcgcag gacgccccat
     2341 acgatcgtgc gctgattgat cgctctactt gcatgtctca ttatctcggt ttgaccaact
     2401 tgcatcattg tctttcctgt tgccaggcaa ctactttaca cagactatac atgaccagcg
     2461 ctgttatgcc aagcaagagc aggatgcttt ccatcccttg cacggcggtg gcatgttcga
     2521 tcagttgcag attatcgcca aagaaacccg atgccatgta cccgccgacc ggcgtgagaa
     2581 tggtggtact caggctggga atcaggctgc tcggaccgag gatattaccc cagaaccaat
     2641 agccggtaaa aagaacctgg tagagcggca cccacatgat agaggggcag gcaatcgaga
     2701 aggcgctaac gaagagcaga ccgggcagcg caacaaccgc aaacttcaac agcccgtcgg
     2761 gaagagccat caggttatgg ctgctgaaga gcaggaaacc gatcccagca caatcgtaga
     2821 gaaatagggg gagaagcgca gccagcgagc ttcccacgta tttgcccaac atgcgtgtgg
     2881 ttgtagcgct cggtaaggtc agcaggattt cttcgacttt tcttcgccgg tcacgcgcca
     2941 aacgatcggc cagcaagacg ccgtatacga tgggaaacac aaacatcaga ttgcgagtcc
     3001 actcggctga cagcgccata attgactggt tatatgggtt gaatatcagc gtatagaatg
     3061 gcccgcctct gccgagtctg acgaagagga ggctcagaca gagataggcg atccacagga
     3121 cgggccgacg tatttgcatg agaaattcgt attttacgac gccggaaaac gccttcagct
     3181 gttcgttcat tcttcgctgg ctcctttcag caacccctcc gggttgcgca gcagaagcca
     3241 ggcgagtacc agcaataaaa ggccggttcc caggatttca cagcgcgagg tcagccatgt
     3301 ccagaacaca tcgttggaga tgaacggcga cggaaagaga ataagcgtcg ttggaaacag
     3361 caatacggga tgaagccagg gaatagccgc caggccatcc ttgaagataa tttcgagaat
     3421 ccagatgcca cctactatag cgctgctggc cgtgcggctg cgcagcagca gcgccaggca
     3481 aagtcctata gccaccatcc agcataaagg cgcaagccag gttaactggt tggtcagaaa
     3541 tgcgagcgcc gttggccagc tattcaattg ctgagggaca taggtcaggc gcagcgcgga
     3601 gagaagcgcg ctggcaagca gcgctatcgc gatcgcccag ccggcgacga gcgccatacg
     3661 tttcacagcc gtcgctgcat aagctcgtgg gacggtcagc tgcaattcga gcgcgggatc
     3721 gtggctggcg atggtcgcga ctatcatgcc cgttgccaga ggcagcagca tctccaaccc
     3781 acccgaaaga aaacgcgccg ggtcaatctg tcgcgtggct aagaatgcgg cgaacagggc
     3841 gaaaccggtg gccagcagta caggggtgag aataacgcgc ttccccatca agcgcagttc
     3901 ataatagagg cgttccagtg tcatacgata tatccttcaa cgctggttat atgtgctaga
     3961 tgctcccgat aagcgtggct ggcgcggcgc gtagtagatg gcgaaggcca gcagagccag
     4021 ggcggccagc accgtccagg gtgtggtctg cccgatagtg tcgcgcagga attgcagcgc
     4081 cggaaccacc ttttcaacgg atgtggccat cgcctgcagc acctcgatga ccagcgaggc
     4141 ggcgatgccg accgccgagc caagcatgac cgagaacgag aggctgatgg atgccagcag
     4201 cagcatgggg ccgaaccaga gctgcgcgaa gtcccacacg ttcccgccgg tgatggaggc
     4261 gataatgccc gaagtaatgg cggcaagcaa gaaattgtgg ccgattacca ggatcatgcg
     4321 gcagatcatc atcacacgga tcgatgtcgg cgttgataga attacctcgt acccagcgtc
     4381 tttttcggcc tggtagataa aggcgatgcc cgctgccgcc acgacggtcg tgaagagcgc
     4441 cagcactccc tcgatgctgc gttcatgggc gtggatgctg aatgggtcgc tgaggaacac
     4501 cagcacgagc attagtatat tgaccaacac cgaggcaatc cagatgcttt tgtggatgag
     4561 cggtatctgc cgggtgaaga ccagccacat atgttgcgcg atctcttgaa gcgtgtgacg
     4621 aggcgcttgc gcctgctgaa tctccatcgc gtcgagtttc tcccagacac gactcatggt
     4681 atgcgccgcc ggtagtggga gcgcttcttc ctctgcatga gttgctttgc tgatagcctg
     4741 ccatgacgcc aactcggcct cgcaggtctg gcagagcgcc aggtgagcgc gtacttccgc
     4801 gcctgagtgg gcgtcgagcc ggccattcag ataggcgggc aacaggtcgg atatatgccc
     4861 ggtgcgcgtg agacggttct tctggacgct gtcgtgcgat gcttgctgcc ggggcagtga
     4921 atgttcgttc atcggtgcgc ctcctctctc gcgtcgagca gtgcgcgcag tgcgcgccgg
     4981 gcattgttga gacggctctt gaccgtgcca agtggcacat ctaggatcgt cgccgtttcc
     5041 tggtaagaga gttcctgcac gaagattagc gccagcactt cgcggtgata gggcgcgagt
     5101 cgctggaaga gttccgtcaa ttcatcgcaa gcgatgctgg cgagggtcag cgcctccggt
     5161 tcggggtcgc tggcagccat ctccgccatc tcgctttcat ccaccagcag cagcttgcgc
     5221 tggcgcagcg tattatgggc ctgcctgcgc gcaatgccga tcaaccacgt gcgtacgctt
//
LOCUS       DASZAK010001101         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_133125, whole genome shotgun sequence.
ACCESSION   DASZAK010001101 DASZAK010000000
VERSION     DASZAK010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133125"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VGT44_19465"
     CDS             <1..374
                     /locus_tag="VGT44_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583048.1"
                     /translation="ECLVMDMVTTPTVPPDKRSEYGRTMISHLLRELQAWGEQGVEIT
                     KFHAVSSTPAGQHILASAKFVSGTKITAGRVTFELDVSNSDAIWLRGYKAALEQWKLR
                     QKVPATSSPSAGKTRGASINP"
     gene            complement(601..1917)
                     /locus_tag="VGT44_19470"
     CDS             complement(601..1917)
                     /locus_tag="VGT44_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583049.1"
                     /translation="MSLMLMICRAIIAGAFWIATRLSNEIKISGRQYDNGAARTYLGM
                     THKRDLDPMVLIPTLVFHHGWRRLRGELRFALRGDGFSPGYLGRIVMQPRWLSRLLRV
                     LAIGPVLRWLGAYSVEALLRPGEEWIREALQNGGDRPAREVIAPFLLEALANTSGESY
                     EQIADARLSHLLSWQYHLPLQHFYGAEAIIGAVRRPIQQRHVTRIKSELAELDEWLWG
                     GGSLYSSPEGHLTPNGRFSSINAAFHRLMRAAPPDTRVVPIVLIYDFMTTQRLRIFID
                     FAPAIEHTPALPLDKLDAALLTAWRRYARFTCTQLASGFLRQASQEGLTAFSLDDLAK
                     NIHVQANRLALEGRLVDARLLSLRGARKRARSYLAYAERRLLVQKDRHGAYLPTVAES
                     PMRLRPREVGYDQAPLAYACNELEDMLAFSFDSVTVMITSPGKRRI"
     gene            complement(2035..2553)
                     /locus_tag="VGT44_19475"
     CDS             complement(2035..2553)
                     /locus_tag="VGT44_19475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583050.1"
                     /translation="MSYNPYSDETVKARAETYPPMDELQLKQPVQAYAMPTSPVTETP
                     RPPEQAEAQGSQLKAKSTKFAIGKFNDYLSWFVMVLETMLALRFLFRLIGADLNNPFF
                     GFLYALTSILLFPFENIVGSPVISSAHQNAVQLVEFPTLIGMLVYFLFYYALRRFFHL
                     LISRPEDEAAAE"
     gene            complement(2649..>3167)
                     /locus_tag="VGT44_19480"
     CDS             complement(2649..>3167)
                     /locus_tag="VGT44_19480"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEV2583051.1"
                     /translation="RFLAALKDRFAHQALPAWRDRQWERFSLRMQPLARPLPSFRLQW
                     VLFAWITLMFGLNFLIAKPQLNIFWANHEPDGREQHIEQLLSMIPPDASVSASDDLNP
                     HLSERQLLAVFPSVCLDARCDQVVQYVIVDLNSLTTENQATAARELNSLHNQYRVLAQ
                     AGGVVLLIRRSA"
BASE COUNT          728 a          870 c          942 g          627 t
ORIGIN      
        1 tagaatgcct cgttatggac atggtgacga ccccgacagt accaccagac aagcgttcag
       61 aatatggtcg cacgatgatt tcacatcttc tgagagaact acaagcctgg ggagaacagg
      121 gagtagagat caccaagttc cacgcagtga gcagcacgcc cgcagggcag catatcctcg
      181 caagcgcaaa gttcgttagc ggaaccaaga taacagcagg ccgcgtcacc ttcgagctag
      241 atgtcagcaa ttctgatgca atatggctca gagggtacaa agcagctctc gaacagtgga
      301 aattgcggca gaaagtaccg gcaaccagca gcccatcagc tggcaagacg agaggggcat
      361 cgatcaatcc atagtttcca caaaaaccat ataaaccata cattaagtta agaagcgctt
      421 ttcagatgca gctcgacaca cgcgccggaa aagcgctcta attcaattcc ggttcgctgt
      481 tgccctatct taactcccga caccttgcca aatctcgaca ggaatagacc cgttctagga
      541 gccagttcat agcaagcgag cagccgaaga gccgaaccgt gagaaccagt taagatacgg
      601 ttaaattcgc cgcttccctg gtgaggtaat cattaccgtt acactgtcga agctaaaagc
      661 cagcatatct tcgagttcgt tgcaggcata agccagtggg gcctggtcgt agccgacctc
      721 gcgggggcgc aggcgcatgg gtgactctgc aactgtcgga agatacgcgc cgtgtctatc
      781 tttctgaacc agcaggcgtc gttcagcgta ggcgaggtag ctcctggcgc gtttccgcgc
      841 tccgcgcagg gagagcaggc gcgcgtcaac gaggcggcct tctaaggcca gcctgttcgc
      901 ctggacatga atgttcttcg ccaggtcgtc gagcgagaac gccgtcagcc cttcctggct
      961 ggcctgccgc aagaagccgc tggcgagctg tgtgcaggtg aagcgagcat agcgccgcca
     1021 ggcggtaagg agggcggcat cgagtttgtc gagcggcagc gctggagtat gctcgatggc
     1081 gggcgcgaag tcgatgaaaa tgcgcaggcg ctgcgtcgtc atgaaatcgt agatcaagac
     1141 gatgggaacg acgcgtgtat cgggcggggc tgcccgcatc agccggtgaa aggcggcatt
     1201 gatcgaactg aacctgccgt tcggcgtaag atgcccttcg ggcgaactgt agagggaacc
     1261 gccgccccag agccactcat ccagttcggc cagctcggac ttgatgcgcg tgacgtggcg
     1321 ctgctggatg ggacgccgga cagctccgat aatcgcttca gcgccgtaaa aatgttggag
     1381 tggaagatgg tattgccagg aaagcaggtg tgagaggcgc gcgtcggcga tttgctcgta
     1441 ggattcgccg ctggtgttgg ccagcgcttc gagcagaaat ggggcgataa cctcgcgggc
     1501 tggccggtct cctccgtttt gtagcgcctc gcgaatccat tcctcgccag gccgcagcag
     1561 cgcttcgacc gagtatgcgc ctagccagcg cagcaccggg ccgattgcca gcacgcgcag
     1621 gagacgcgac agccagcgcg gctgcatcac gatccgtcca aggtagcctg gcgaaaaacc
     1681 gtcgcctcgc agcgcgaaac gcagttcgcc tcgcagcctg cgccatccgt gatggaaaac
     1741 gagtgtggga atcagcacca tcgggtccag gtcgcgcttg tgcgtcattc ccaggtacgt
     1801 gcgcgccgct ccattgtcat actggcgtcc cgatatcttg atctcgttgc tgagccgcgt
     1861 cgcaatccag aacgccccgg cgatgatcgc ccggcagatc ataagcatta gtgacatgat
     1921 ttgcttcata gcacaccgtc gctctcgtcg tcggatgctt gccaatacgc tgcatggcga
     1981 ccgcccgggt cgccatgcat tcatgcttgc tgtagaactg ttttgtatcg ccgttcattc
     2041 cgccgcagct tcatcctctg gcctggagat gagcaggtgg aaaaagcgcc tgagcgcgta
     2101 gtagaacagg aagtagacga gcataccgat taaggttggg aactcgacca gctgcaccgc
     2161 gttctggtgg gcagagctaa tcacgggaga tccaacaatg ttctcgaagg gaaatagtaa
     2221 gatggaggtc agggcataga gaaagccgaa aaacgggttg ttcaaatcgg ctccgatcaa
     2281 ccggaagagg aaacgcagcg ccagcattgt ttcgagaacc ataacgaacc aggacaggta
     2341 atcgttaaat ttgccaatcg cgaatttggt ggactttgct ttaagctggc tgccctgagc
     2401 ctcagcctgt tcaggcgggc gcggtgtctc agtcacaggc gatgttggca tggcatatgc
     2461 ctgcaccggc tgcttgagtt gcagttcatc catgggagga taggtttcgg cacgcgcttt
     2521 gacagtttcg tctgaataag gattgtagga catacgtaca tgctcctcca tgatgttgat
     2581 gaattgccca cgcggtggat agccgcagaa taatatgctc ctgtatcgat tataacatga
     2641 tttgcccgtt aagcgctacg gcgaatcagc aaaacgacgc ccccggcctg cgcaagaacg
     2701 cgatactgat tatgcaggct attcaattct ctggccgccg ttgcctggtt ttctgtcgtc
     2761 aagctgttga gatcgacaat cacgtattgt accacctggt cgcagcgcgc atccaggcaa
     2821 accgagggga agacggccag caactgccgc tcgctcaggt gcgggttcag gtcatcgctg
     2881 gccgagacgg aggcgtcggg tggaatcatg gacagcagtt gctcaatatg ctgctcgcgc
     2941 ccatccggct cgtgatttgc ccagaagata ttcagctgcg gcttcgcgat caggaaattt
     3001 agcccgaaca tcaacgtaat ccaggcgaac agaacccact gcaaacggaa agaggggagc
     3061 gggcgcgcca ggggttgcat acgcaggctg aagcgttccc actgcctgtc ccgccatgcc
     3121 ggaagcgcct ggtgcgcgaa gcgatctttc aaggcggcca ggaagcg
//
LOCUS       DASZAK010001102         2214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_311814, whole genome shotgun sequence.
ACCESSION   DASZAK010001102 DASZAK010000000
VERSION     DASZAK010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2214
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311814"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..>2214
                     /locus_tag="VGT44_19485"
     CDS             175..>2214
                     /locus_tag="VGT44_19485"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583052.1"
                     /translation="MSDAGTWEERNAHYLSVALTWLRLRLERLAQHDMSPGPIPQPKQ
                     EPPQKHSFLGRPAGEQTAPTPVIPVTAMTSATLDERIKQLEAELAAAEATSDPPALIA
                     LSQRLGLSAFEREILFLCVALELDTRIAALCARAQNDTNRPYPTFALAFMLFDDPPWE
                     SLAPDRPLRHWHLIEINQPGALALTNSPLRADERIVNYVKGLNRLDDRIAALTEPLSL
                     AKDTLDLPPSQQEIVQRIVHYWQQMAVMAPMASTSSFPYVQLVGSDEGSKELVALRTT
                     AQLNRQLYCLPMHMFPVQGTEIDLLARLWQRESLLLPLALYLDAHEMDASATSEGQVQ
                     ALSRFLAHNGGICFLSLRESLPRLDHAHLVLDVARPMPAEQHEAWTTMLREKAGTIPA
                     LLTSHFHLNTTTIERVARMALADTKEDEHELAERLWDACRLSVRPHLDMLAQRLDSAV
                     TWDDLVLSQEETTMLHQVADQVRQRSKVYEVWGFAARMKRGLGINALFAGESGTGKTL
                     AAEVIANDLHLSLYRIDLSAVVSKYIGETEKNLRRLFDGAEDGGALLFFDEADALFGK
                     RSEVKDSHDRYANIEINYLLQRMEAYRGLAILATNMKSALDTAFLRRLRFVIDFPFPS
                     LVERKRMWQKVFPQEMPKEQLDYDRLARFNLTGGGIRNSALNAACNLWRWFVAMGT"
BASE COUNT          476 a          666 c          622 g          450 t
ORIGIN      
        1 cttgccatta caatcgccga atgctcccgc gacgcagcaa ctggtgttcg acgtgccaaa
       61 cctcaccgct ggcgactact ttgtgcgtct gcgcgtcgat ggtatcgata gtctgctggt
      121 aaatcaaagc accacaccac caatgtttga tcaatcgcag agagtgacga tacaatgagt
      181 gatgcaggaa cctgggaaga gcgcaatgcc cactacctct cggtggcttt aacctggcta
      241 cgcctgcgcc tggagcgact ggcacagcac gatatgagcc ctgggccgat tccgcagccg
      301 aagcaggagc caccacagaa acatagcttc ctgggccgac cggcgggaga gcaaactgct
      361 cctacccctg taattcctgt aaccgccatg accagcgcaa cgctcgacga aaggataaag
      421 cagcttgagg ccgagctggc tgcggcagag gcgacgtcgg atccgcctgc actcattgcc
      481 ctgagccagc gactgggcct ttcagcgttt gagcgtgaaa tcttgtttct gtgcgttgcc
      541 ttagagcttg atacgcgcat cgcggcgctg tgtgcccgcg cccagaatga cacaaaccga
      601 ccctatccaa ctttcgctct tgcgtttatg ctctttgatg atcccccatg ggaatcgctg
      661 gcaccagatc gccctctgcg ccattggcat ctcatcgaga tcaaccagcc aggagcgctg
      721 gcgctcacaa acagcccgtt gcgggccgac gagcgcattg tcaactatgt caaaggactc
      781 aacaggctcg atgatcgcat tgctgctctg acggagccgc taagtttggc aaaggataca
      841 ctcgacctac ctccttcgca gcaggagatt gtgcagcgca tcgtccatta ttggcagcag
      901 atggcagtaa tggctccaat ggcttcgacc tcatcgttcc cttatgtgca actggtcggt
      961 tcggacgagg gcagcaagga gctggttgct ctgcgcacca ccgcccaact gaataggcaa
     1021 ctctattgcc tgcccatgca tatgtttccc gtgcagggaa ctgagatcga tctcctggcc
     1081 cgactctggc agcgcgagag cctcctgctt ccgctggccc tctacctgga tgcgcacgag
     1141 atggatgcct ctgctaccag cgagggacag gtgcaggcac tcagtcgctt cctggcgcat
     1201 aatggcggta tctgtttcct cagcctgcgc gagtcgctcc cacgcctgga tcacgctcac
     1261 ctcgtgctcg acgttgcccg gccaatgcct gctgagcagc acgaggcatg gactactatg
     1321 cttagagaga aagccgggac gataccggca ctgctgacaa gccactttca cctcaatacg
     1381 accaccatcg agcgagtagc acggatggca ctcgcagata cgaaggaaga cgagcatgaa
     1441 cttgctgagc gcctatggga tgcctgccgg ctcagcgtac gaccccacct ggacatgctc
     1501 gcgcaacggc tggacagcgc cgtgacgtgg gatgaccttg tactgtcgca ggaggagacg
     1561 acaatgttac accaggtggc agaccaggta cgccagcgca gcaaagtcta cgaggtatgg
     1621 gggttcgccg ctcgcatgaa gcgcggactc ggcattaacg ctctcttcgc aggcgaaagc
     1681 ggcacgggca aaacgctggc ggcagaagtg atcgccaatg atctgcatct tagcctctat
     1741 cgcatcgatc tctcggcggt ggtcagtaaa tatatcggcg agacggaaaa gaatctgcgt
     1801 cgcctgtttg acggtgcgga ggatggcgga gcgcttctct tcttcgacga agccgatgcc
     1861 ctcttcggca agcgcagcga ggtcaaggac agccatgatc gctacgccaa catcgagatc
     1921 aactatctct tgcagcgtat ggaggcctat cgcggcctcg ccatcctggc caccaatatg
     1981 aagagcgccc tggatactgc cttcctgcgc cgcctgcgct tcgtcatcga tttccctttc
     2041 cccagcctgg tcgagcgcaa acgcatgtgg cagaaggtat ttccacaaga gatgccaaag
     2101 gagcagttag attacgaccg cctggcccgg ttcaatctga cgggtggtgg cattcgtaat
     2161 agcgctctga atgcggcctg caacttgtgg cgctggttcg ttgcgatggg cact
//
LOCUS       DASZAK010001103         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_668681, whole genome shotgun sequence.
ACCESSION   DASZAK010001103 DASZAK010000000
VERSION     DASZAK010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668681"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..1323
                     /locus_tag="VGT44_19490"
     CDS             259..1323
                     /locus_tag="VGT44_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HEV2583053.1"
                     /translation="MEQMEPTKVQAKARMPRGKFNGINAVADKSGIIAAAAMDQRGSL
                     RKAIAKAKGSDASEAEVSEFKVLVTEVLTPYASAILLDPEYGLEAVKHRAKQAGVFLA
                     YEKTGYDATVKGRLPDLLPEWSVRRLVDAGADVIKILLYYDPDDTEQINTVKHAFIER
                     VGAECRAYDVPFFLEAIAYSDEIGDENGFAFAPVKPSKVKKYMQEFSQPQYGVDVLKV
                     EVPVNMRYVEGSKANPDGQVAYSREEAKQHFREAAAATRLPFIYLSAGVSDEVFRETL
                     ELATEADTPFSGVLCGRATWQDGIPVYGQGGAAALRSWLEDQGVRNIQALNEVLMKGA
                     KPWWDFYGGKENIELVDAQV"
     gene            1372..1839
                     /locus_tag="VGT44_19495"
     CDS             1372..1839
                     /locus_tag="VGT44_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV2583054.1"
                     /translation="MKAEDVMTTRVITVTEDQTKQQAARLLSQHRISGLPVVNNDHVV
                     VGVVTEYDVIGKEGQTVGEIMTRGVISVTPDTDLEEVSHILVHQRIKRLPVIDRGKLL
                     GIVSRADLVREVAMSWVCPVCGEVVRSEEQPERCPRCGARNVPASLEPVPPGS"
BASE COUNT          486 a          523 c          658 g          419 t
ORIGIN      
        1 ttgattttac cgtgatgtgt gtacctcttt taaagaatgt gtgagtttta aactgatatc
       61 gtatatcgtg ggcgagattt aatagtagcg tgcaactgat gaaggccagc ctggggctgg
      121 ataacaatgc cgtcgggctc ctcgttcgtg atccagagca atatgagcga ccccgacgtc
      181 tcgaagtacc tctaaaaatc ttcgtgctga gtaaatccag ggggaggagc gcagactcct
      241 cctctcacgg gagaagggat ggaacagatg gaaccaacca aagtacaagc caaagcgcgg
      301 atgccccgcg gaaagttcaa tgggatcaat gccgttgccg ataaaagtgg gatcatcgcc
      361 gccgcagcga tggatcagcg tggttccctc cgcaaagcta ttgctaaggc gaaggggagt
      421 gatgcctctg aagccgaagt gagcgagttc aaggtgctgg tgaccgaggt actcacgcca
      481 tacgccagtg cgattttgtt agacccggag tacgggctcg aagcggtgaa gcatcgggcc
      541 aaacaggctg gggtgtttct cgcttacgaa aaaaccggtt acgatgccac ggtcaagggg
      601 cggctgcccg atctgctccc ggaatggtcg gtgcgacggc tcgtcgatgc gggcgccgac
      661 gtaatcaaaa tcttgctgta ctatgatccc gacgatacgg agcagatcaa cacggtgaaa
      721 catgccttca tcgagcgagt gggggccgaa tgtcgtgcct acgacgttcc ctttttcttg
      781 gaggctattg cctacagcga cgagattggc gatgagaacg gcttcgcgtt cgctccggtc
      841 aaaccctcca aggtgaagaa gtacatgcag gaattctccc agccacagta cggagtagac
      901 gtgttgaaag tcgaagtccc ggtgaatatg cgctacgttg agggctcgaa ggccaaccca
      961 gatgggcagg tcgcctatag tcgggaagag gccaagcagc acttccgcga ggccgcagcc
     1021 gctacgcgcc tgccgttcat ctacctcagc gccggtgtga gcgacgaggt cttccgcgag
     1081 acgctggagc tggccaccga agctgatacg ccgttctctg gggtgttgtg tgggcgggcg
     1141 acgtggcagg acgggattcc cgtgtatggg caaggcgggg ccgcggcatt acgaagctgg
     1201 ctggaagatc agggggtacg gaacatccag gcattgaatg aagtgctcat gaagggggcg
     1261 aagccgtggt gggacttcta tgggggcaaa gagaacattg agctggtaga cgcgcaggta
     1321 taacgcctca gtccttgcgc taaacgaaac accgaggaag gagatgcagg catgaaagct
     1381 gaagatgtaa tgaccaccag ggtgattaca gtcacagaag accagaccaa acagcaggcg
     1441 gcgcgcctgc tatcgcagca ccgtatcagc ggtctacccg ttgtcaacaa tgatcatgtc
     1501 gtggtgggtg tggtgaccga gtacgatgtc attggcaaag aagggcagac ggtcggtgag
     1561 attatgaccc gtggggtcat cagtgtgact ccagataccg acctggaaga ggtgagccat
     1621 atcctcgtgc atcagcgcat taagcgttta ccagtgatcg atcgaggcaa gttgcttggt
     1681 attgtcagcc gtgccgacct ggtcagagaa gtggctatga gctgggtgtg ccctgtgtgc
     1741 ggggaagtgg ttcgtagtga ggaacagccg gagcgttgcc cccgctgcgg agcaaggaat
     1801 gtgcctgctt cgcttgagcc ggtgcctcca ggatcatgag aagccgattc tactggtatc
     1861 cttcatattt cgcacaagcc gtaggggccg ggctacgccg ctcggctact tcgcctttga
     1921 caagaaagga tcaggtgcct cttggacacg acaagcaaac aacaagtgga tgcgcagggc
     1981 gctgcaactg tccgcgagca agcacaggcc gccatccctc caggggaaga tcgcgatacc
     2041 ctgctccgct actaccacca gatggtgctc attcgtcgct ttgagg
//
LOCUS       DASZAK010001104         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_456058, whole genome shotgun sequence.
ACCESSION   DASZAK010001104 DASZAK010000000
VERSION     DASZAK010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456058"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1027..1488
                     /locus_tag="VGT44_19500"
     CDS             1027..1488
                     /locus_tag="VGT44_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583055.1"
                     /translation="MKHKVIRITLVAIEAFIGLGAIFGGIALLTGAFNFEQWLPVAWL
                     QGTPFSGYTIPGLLLLIVVGGGMLLAATTVFVKREWAVLLSAAMGLVMIGFEVVEVAI
                     IDRYTQAIVPPTIVQQILMSTLGLLIFGLAGYLWMREYRSHSFLSRHISHA"
     gene            1527..>2861
                     /locus_tag="VGT44_19505"
     CDS             1527..>2861
                     /locus_tag="VGT44_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583056.1"
                     /translation="MSTDIEQVDEKVVGGQAPKRRIGYEILDALSGLPLFATAPLYRH
                     WHVRWGATDDEVRSSMPGDELVPQASLDATRAITIDASPERVWPWIVQMGYRRAGFYT
                     YDLIDNDGYPSADRVLQEYQHIEVGDWAFPMNGFFGIRFPLNETTAFKVKAFETNKWL
                     LWEKPDSTWAWLLKPLPGGRTRLISRQKAHYPEPSTMILMEFGDPPMVRRVLKGIKAR
                     AERVGAAPVAPASADHRIPSLIHVFTRATRPIRAAAKTAVFFLKVLPMLPSKPVNWVT
                     SPPVIERVRYPTLHGQVEGELYRPSTGGPYPGIVMCLGVVPFGVDHPQVPRLEEALAR
                     SGFAALIYWSPAMRDLRLDPEDIENIALAYHWLLEQPYVDPGRSGLFGTCVGGSFALM
                     AAASPLIRDRVAFVGAFAPYSSMWTFAQDIASATRPGREGRSPWQVDQLTRKV"
BASE COUNT          625 a          796 c          805 g          635 t
ORIGIN      
        1 tgtcgttggc ggcacgcgcc atgatgtcgc cgacgcgctg gcgattgtgg aacgtctgac
       61 ttttgcccag caaactgaga tacaattcgg cacgagcgtc acgcgccagg cgtttgccca
      121 gtagttctgg aaagaaccgg gcgcacaagt cgaggccccc accgattatg atgacggcaa
      181 gtagtgcgag ggaaactact agcaagcgat tacgtgccaa ctcaccttgc aatacgatgt
      241 taaaggctac tccggtgagt actggaatga ctgcgtagag tatattggtc aaaatcacgg
      301 caaacatgaa gctgagaatt aaatgcttgt aacgcatcaa gtgagagata atccagcgta
      361 cggggcttaa gtgattgtag tggtattcat cggcaaccgt aaattcgcgt tttagcaagt
      421 tccacctttc ccattagcag ggaattcctc gtagagctac gagctatctt agatccacag
      481 gaggatagta tacaacgatt cggtttactc tcctagtcct gcgacgtatc ggtaagaggc
      541 aggaccacca acgcgaggtg tctatcgctt tgaggattgt gccaacgaag gttgagctgc
      601 attttcaagc tgtttccttc gacgacaaaa tgatcgattt gtgcaaagat cctgggattt
      661 ctctggccga gacgaagaga gggccacatc taagtaggga gtaattggcg ctcaaccttc
      721 agagggccac ggtgagagcc cgcatctttt ctcgaagcag aggctatgga gtagcctttc
      781 ccctgcacag aatgacacgt tactcctatt acttaccacc ttttctcccg ttcttcaact
      841 gcgtgtcatc attgtgcggc gtggagtcca gtccgaaatg tgatcattct gtgagaatgg
      901 aatgatccat tgtgagggca atgtgaggcc aatcgacgat cctttattca gaaatagagt
      961 gctgttccat gtgcaagaca ctggctggag aggaagcgaa ttctaccaga gcaaaggaga
     1021 ataatcatga aacacaaagt cataaggatc acactcgtcg ccatcgaagc ctttatcggt
     1081 ctgggcgcca tcttcggcgg catcgctctt ctgactggag catttaactt tgagcaatgg
     1141 ctaccagtcg cctggctaca aggcacgcct ttcagtggct acacgatccc tggcctgctc
     1201 ctcttgatcg tggtaggagg tgggatgctc ctggccgcaa ctactgtctt cgtcaagcgc
     1261 gaatgggcag tgctcctctc agcggcgatg ggcctagtga tgattggctt cgaggtcgtc
     1321 gaggtcgcca tcatcgaccg atatacgcaa gctatcgttc ctcctacaat tgtgcaacaa
     1381 atcctcatgt ccacgcttgg tctgctgatc tttgggctag cagggtacct gtggatgagg
     1441 gaatatcgca gtcacagctt tcttagcagg catatcagtc atgcctaatg gaccaggagc
     1501 aaaaccaatc taactggagg tgaaagatgt ctacagatat agagcaggtt gatgaaaaag
     1561 ttgtaggagg tcaagcaccc aagcgcagga tcggatatga gatactcgac gcgctcagcg
     1621 gtctaccgct cttcgccact gccccactct accggcactg gcatgtgcga tggggggcga
     1681 ccgacgacga ggtgcgaagc tccatgcctg gggacgagct cgttccccag gcgtccctcg
     1741 atgccacccg ggccatcacc atcgatgcat ctcctgagcg tgtttggccg tggatcgtcc
     1801 agatggggta ccgcagagcg gggttctaca cctatgacct gatcgacaac gatggctacc
     1861 cgagcgccga tcgcgtgctc caggagtatc agcacatcga ggtcggcgac tgggcttttc
     1921 ctatgaatgg cttttttggc atccgcttcc cgctgaacga gacgacggcg ttcaaagtga
     1981 aggccttcga gaccaacaag tggctactct gggaaaagcc cgacagcacg tgggcctggc
     2041 tactcaagcc cttgcctggc ggccgaactc gcttgatctc gcgacagaag gcgcactacc
     2101 cggagccttc cacgatgatc ctcatggagt tcggcgaccc tccgatggtg cgaagggtcc
     2161 tgaaaggcat caaggcccgc gccgagcggg tgggcgcagc accggtggca cccgcctcag
     2221 ctgatcacag gatacccagc ctgatccacg tcttcacccg tgcgacgcgg ccgatccgtg
     2281 cagcggccaa gacggcggtc ttctttctca aagtcttacc gatgctgccg tccaaaccag
     2341 tgaactgggt gaccagccca cccgtgatcg agcgtgtgag gtatccaacg cttcatggcc
     2401 aggtcgaagg cgagctctac cggccatcga caggcggtcc ctatcccggc atcgtgatgt
     2461 gtctaggcgt cgttcctttc ggcgtcgatc acccacaggt tccgcgcctc gaggaagccc
     2521 tggcccgttc aggcttcgcg gctctcatct actggtcgcc tgcgatgcgg gacctccgcc
     2581 tggatcccga ggacatcgag aatatcgccc tggcgtatca ctggttgctt gagcagccgt
     2641 acgtggaccc tgggagatcc ggtctgttcg ggacgtgtgt gggaggctcc ttcgccttga
     2701 tggcagctgc cagtcccctc attcgtgacc gcgtcgcctt cgttggggca ttcgcgccct
     2761 attcctcgat gtggacgttc gcacaggaca tcgccagtgc cacgcgcccc ggcagagagg
     2821 gacgttcccc ctggcaggtc gatcagctaa ctcgcaaggt g
//
LOCUS       DASZAK010001105         8904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_133248, whole genome shotgun sequence.
ACCESSION   DASZAK010001105 DASZAK010000000
VERSION     DASZAK010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133248"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1510
                     /gene="uxaC"
                     /locus_tag="VGT44_19510"
     CDS             110..1510
                     /gene="uxaC"
                     /locus_tag="VGT44_19510"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEV2583057.1"
                     /translation="MHNTRYFSAEPSVRAIATQLYSQVATSPLLCLHGHVDPRVFADP
                     QYSFGTPVDLLIIPDHYVFRMLYSQGVALEQQGIPRRDGQPVEQDHRHIWQAFAERFY
                     LFRGTPTGTWLRDELRDIFGIEEKLTGATAQRIYDRIVEQLATPAFRPRSLYERFNIQ
                     TLCTTDAATDALTHHAALRASGWQGDVRPTFRPDAVVNICAPGWSEHIAALSRVSGID
                     VHDFASYIAALEDRRAFFKSMGATATDQGVLTPYTGELSGREADVIFQRALRGEADAN
                     DEARFTGHMLMEMARMSIEDGLVMQVHSGSLRNHNAPLYEGFGPDMGADIPTRLEYTR
                     NLRPLLNKYGNDARLALILFTLDESTLSRELAPLAGHYPALKLGPPWWFFDSLNGMAR
                     YFDRVIETTGLYNTVGFNDDTRAFCSIPTRHDLWRRASANWLAGLVAREIIDLDDANE
                     MIYAMAYGLAKDAYKL"
     gene            1520..2341
                     /locus_tag="VGT44_19515"
     CDS             1520..2341
                     /locus_tag="VGT44_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2583058.1"
                     /translation="MNPNTLFDIQGQVAVITGGSGVLGSVMARSLAQAGAKVAILGLH
                     AEKAQAVAASIQEQGGQANGIACDVQDRASLEHALEQVARSFGPPDILVNAAGGNAPR
                     ATTSATQTFFDLDQAAIEHVVGLNFTGTLLSCQVFGRAMAERGQGCIVNIVSMNALRP
                     LTRIPAYSAAKAAVANFTQWLAVHLAQECSPRIRVNAIAPGFFLTEQNRYLMTTPQGD
                     WTPRGQAVIQHTPANRLGAPEDLVGTLLWLVSPASAFVTGVVVPVDGGFSAFSGV"
     gene            complement(2356..3417)
                     /locus_tag="VGT44_19520"
     CDS             complement(2356..3417)
                     /locus_tag="VGT44_19520"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2583059.1"
                     /translation="MPPRLTDWSIALAAVLAFTLGIISLVSGFPQEWFIFALHGIAGV
                     WLLLLLWGKLRRVWPRLIHLRRWDRRTVFGLLALLLVTLALVSGIWWVGGGNMYFVGF
                     NLLNWHILLGFVVTAAIVIHMFARAKRLRKRDIIGRRRMLHFSALFVGSVSIWPAQQL
                     AERVLKLPGARRRFTGSYENSSYAGNAFPTSSWVADAPRPIDVQTWRLSLGGAVTTSR
                     DFSYDELVAAQDKEEATLDCTGGFFSTQHWRGIRVGHLLDRAVLHTDARYVSFVSVTS
                     YRWSLPIEEARTALLATHVDEEPLSHEHGFPLRLVAPGRRGFEWIKWITRIEVLTAPD
                     PGQVLSIFTSSFTDAGRGG"
     gene            3654..3968
                     /locus_tag="VGT44_19525"
     CDS             3654..3968
                     /locus_tag="VGT44_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583060.1"
                     /translation="MNELSTEENKHMSSVRMSATSAYSARIQEAMRQNKLRTSDYSLV
                     MSIMRAISYGDTEEKWKTEQQRAVKSLLEEPTDKKMAHAEAANLYEQTIAGLQELVLW
                     PW"
     gene            4092..5102
                     /locus_tag="VGT44_19530"
     CDS             4092..5102
                     /locus_tag="VGT44_19530"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEV2583061.1"
                     /translation="MAWFASVAASAAVVGVAIGSGTLLLGHRFIKEFTRPGVTINRGT
                     PQWGGWTFPESVIEPPPELQRSVTFQAADGALLCGEFWAQTRSAPTVIISHGFHLPCE
                     HFRSVAALEYAHGANILLFDYRGHGKSSQIPTTCGNAEVNDLLAAVDVAASQAETTQG
                     QVYIHGFSMGAAVAILLPPHAAVAGIIADSSYARLDEMTRLLIGQVFDQETSGWRGPA
                     RVLRSLIPSLTRLTFLGGQLLFHLRFRHPLVARPDQAISDHHVKHMVRAPGIIAPPIL
                     LIHARHDPLIALNHAHRLVALARAAGRSIREYYTPCDIHCGSYGYDPRRYMALLLEFM
                     TL"
     gene            5633..5950
                     /locus_tag="VGT44_19535"
     CDS             5633..5950
                     /locus_tag="VGT44_19535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583062.1"
                     /translation="MTLRVLGKDFYPSVFGNGNFATCENLMAATAAGVQIAISVKQET
                     PPFVDVRGKTAYDIQVCTGPTARYIWFKNLDACAELDYGDTAHESSNFVTFTVEPRRE
                     TTG"
     gene            complement(6042..6872)
                     /locus_tag="VGT44_19540"
     CDS             complement(6042..6872)
                     /locus_tag="VGT44_19540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583063.1"
                     /translation="MTTSPQAATLLPIIVPFSERLSETWLLERTYHAQLGSGKELSVP
                     DLTLAMKQLSDFDAPLSLQSSKSKLAERVPLTKSTNFYAPLSTWKEISQADARASYQQ
                     GIPILLYGEHNWEHSQGDSRAWSPNKNMRVISFGNASVKPEAVSGTDYAVCYIDPNLG
                     TFSNTVWKAWFASDTVTLLEGGNHGAITFFGPCVQFPYTTHYTVIAADGLVHDYATSA
                     EALQGFQILPAEGNRSQVQMVSPQFSFYHEVTCPSGVYRIEFFGPRMDKPGYQVEEVA
                     "
     gene            complement(6972..7349)
                     /locus_tag="VGT44_19545"
     CDS             complement(6972..7349)
                     /locus_tag="VGT44_19545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583064.1"
                     /translation="MERAVQIKVITYSSPGQYARDLRHQQQDGWQPVDSVAFQQRRNV
                     AELAVVPLALVRGRKNKLVITYQRVSEYPDTFSGRMQEATARLNARKATQEQHIADIK
                     AADPTSRIGEKIGPKWLGIRRLR"
     gene            complement(7402..8229)
                     /locus_tag="VGT44_19550"
     CDS             complement(7402..8229)
                     /locus_tag="VGT44_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEV2583065.1"
                     /translation="MNLLVLFVVAAIVLVLVIAALSAIRVVQQYERGVIFVLGRLIGA
                     KGPGLFLVPPFISRMAKVDLRIVTLTVPPQEVITRDNVTIRVTAVVYFYVVDPIAAVI
                     KVVNFNQATSQIGQTTLRNVLGQSELDELLAERKKINHDLQAIVDEQTERWGVKVTAV
                     EIKDVELPPTMQRAMAKQAEAEREKRAKVIHAEGELLASTKLAQAAEIIGSQPSALQL
                     RYLQTLTEIAVEKNSTIVFPLPLDLIEPFIHMMRHEASEPAHELLNGSRSSEPSLTR"
     gene            complement(8290..>8904)
                     /locus_tag="VGT44_19555"
     CDS             complement(8290..>8904)
                     /locus_tag="VGT44_19555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2583066.1"
                     /translation="SMQTYLASNKRLSDLADDDLLRQAQSGDPGAFEMLVERYSAMLF
                     RLICRLVGDKHLAHDVLQHVFIQLYRSLPTLIPEGTLKAWLCQVARHRSLDELRRRRP
                     LLFSEIEPFPDGGEYSFLATLPDPDRQPEEQVERRELRQRLLEAIETLPSRYRAVVLL
                     RYNTQWSYRKIGQALRIPETTAKTHFSRAREQLRTVLGSEFAYD"
BASE COUNT         1949 a         2575 c         2491 g         1889 t
ORIGIN      
        1 aactgctgtt catccgcgag ctgatcctat cggccctgtg gcgctcgccg gaacaatggg
       61 ggctgtctgc ggaataatgt agtagactgt caaggtcgat aggagttcga tgcataacac
      121 gcgttatttc agcgcggaac cgtccgtgcg cgccattgcc acgcagctct attcgcaggt
      181 tgccaccagc ccgctgctct gcctgcatgg gcacgtggac ccgcgtgtgt tcgccgaccc
      241 gcagtattct ttcggcacac ccgtcgatct gcttattatc ccggatcact acgtctttcg
      301 tatgttatat tcgcagggcg ttgcgcttga gcaacaggga atcccgcgcc gcgatgggca
      361 gccggtggaa caggatcacc gccatatctg gcaagccttt gccgagcgtt tctacctgtt
      421 ccgaggcacg ccgacgggta cctggctgcg ggacgaactg cgcgacattt ttggcatcga
      481 agagaaactg acgggcgcga ctgcgcagcg aatctacgac cgcatcgtgg agcaactggc
      541 cacgcccgcg tttcgcccgc gctcgctgta tgagcgattc aatatccaga cgctctgcac
      601 gaccgacgcc gcgaccgatg cgctcacgca ccacgctgcg ctgcgggcat cgggctggca
      661 gggagatgtg cgcccgacgt ttcgcccgga cgccgtcgtc aacatctgtg cgccgggatg
      721 gagcgagcat atcgcggcgt tgagccgggt cagcggcatc gatgtgcatg attttgcctc
      781 gtacatagcc gccctcgaag accgccgcgc cttcttcaaa agcatgggcg cgactgccac
      841 cgatcaaggg gttctcacac cctacaccgg cgaactttcc gggcgcgagg cggatgtgat
      901 cttccagcgc gcgctgcgcg gcgaagcgga cgccaacgac gaagctcgct tcaccggaca
      961 tatgttgatg gagatggcgc gcatgagcat cgaggatggc ctggttatgc aggtgcattc
     1021 cggttcgctg cgcaaccata acgcgccgct ctacgaaggc ttcggccccg atatgggcgc
     1081 ggatattccg acacgactgg aatacacgcg caatctccgt cctttgctca ataagtatgg
     1141 caacgacgcg cgcctggcgc tgatcctgtt cacgctggac gaatcgacct tgagccgcga
     1201 actcgccccg ctcgcgggac actaccctgc cctcaaactt ggcccgccct ggtggttctt
     1261 cgacagcctg aacggcatgg cgcgctactt tgaccgggtg atagaaacga cggggttgta
     1321 caacacggtg ggctttaacg atgatacacg ggcattctgc tcgatcccca cccggcatga
     1381 tctgtggcgg cgagccagcg cgaactggct ggctggactg gtcgcgcgtg agatcatcga
     1441 tctcgacgat gccaacgaga tgatttacgc gatggcttac gggctggcga aggatgccta
     1501 caaactttag gagcgccaga tgaacccaaa cacactcttc gacattcaag ggcaggtcgc
     1561 cgtcatcacg ggcggcagcg gcgttctcgg cagcgtcatg gcgcgctcgc tggcacaggc
     1621 gggggcaaag gtcgctattc tcggcctgca cgcggagaag gcgcaggcgg ttgccgcgtc
     1681 tatccaggag caaggcggcc aggccaacgg catcgcctgc gatgtgcagg atcgcgcctc
     1741 gctggaacac gcgctggaac aggtagcgcg ctcgttcggc ccgccggata tcctcgtcaa
     1801 cgcggcgggg ggcaacgcgc cgcgggccac cacgtcggcc acacagactt tcttcgacct
     1861 ggatcaagcg gccatcgaac acgtcgtcgg cctgaacttc accggcacgc tgctgtcgtg
     1921 ccaggtcttt gggcgcgcga tggctgagcg cggccagggc tgcatcgtca acatcgtctc
     1981 catgaacgcc ctgcggccac taacgcgcat ccccgcctac agcgcggcaa aggccgccgt
     2041 cgccaacttc acgcaatggc tggccgtcca cctggcgcag gaatgcagcc cgcgcatccg
     2101 cgtcaacgcc atcgctcccg gcttcttcct gaccgaacag aaccgctacc tgatgactac
     2161 cccgcaaggc gactggacgc cgcgtggtca ggcggtcatc cagcacacac ccgctaacag
     2221 gctgggcgcg ccggaagacc tggtcgggac gctgttatgg ctggtcagtc cggcatcagc
     2281 gttcgtcacg ggcgtggtgg tgccggtcga tggggggttc tcggcgttca gcggtgttta
     2341 attttaccca ttatttcatc ctccgcgtcc ggcgtcggtg aaactgctgg tgaagatgct
     2401 gagtacctgg cctggatcgg gcgcggtcaa cacctcaata cgggtgatcc atttgatcca
     2461 ttcaaagccg cgccgacctg gggcgactaa acgtaaggga aagccatgct cgtgagaaag
     2521 cggctcttca tcgacgtgtg tcgccagcag ggctgtacgt gcttcctcta tcggcaggct
     2581 ccaacgatag ctcgttactg agacgaaact cacatagcgt gcatctgtgt ggaggacggc
     2641 acggtcgaga agatgcccaa cacggatgcc tcgccagtgc tgagtactaa agaagcctcc
     2701 cgtacaatcc aatgttgctt cttctttatc ttgagccgcc accaactcgt cataggagaa
     2761 gtcgcgggat gtagtgaccg caccaccaag tgaaagccgc cacgtctgaa catcaattgg
     2821 acgcggcgca tcggcaaccc aactggaagt ggggaaggca ttgcccgcat agctgctgtt
     2881 ctcgtaggaa ccggtaaagc gacgcctggc gccgggtagc ttaaggacgc gctcggcaag
     2941 ctgctgcgcc ggccaaattg acacgctgcc aacaaacaat gcgctaaaat gcaacatccg
     3001 tcgccttcca ataatatcgc gtttgcgcaa ccgtttggcg cgcgcaaaca tgtgtatcac
     3061 gatcgctgcc gtgaccacaa agcccaggag aatatgccag ttcaacagat tgaacccgac
     3121 aaaatacata ttgccgcctc cgacccacca gatacctgag acaagcgcca gcgtgaccag
     3181 cagcagcgcc agcagtccaa agacggtgcg gcgatcccaa cgccggagat ggatcaggcg
     3241 gggccagacg cgccgcaact tcccccataa gagcagcagc agccacacac ccgctatccc
     3301 atgcagcgca aagatgaacc actcttgcgg gaagccgctc accaggctaa taatgccgag
     3361 tgtgaaagca aggacggcgg caagcgcgat gctccaatcg gtgaggcgcg gaggcataag
     3421 agagaccatc cttatttatc gctattactc gatacgtcga gagtcctggt ggcaaaagca
     3481 gttcaccatg catcaaaatc tatcaaatta tgtcacaaaa cagacgatga gatagtctac
     3541 ttaacttcat tatttttgaa acaaacaata tcccaataga tcattccttg aaggatgtga
     3601 cataatgtac tcgtgacaaa taacgttcat ctaacaagaa acggaaccag ggtatgaatg
     3661 aattatccac tgaagaaaat aagcatatgt cctcagtcag gatgtctgct acatcagctt
     3721 actcggcacg tatacaagag gcgatgagac agaataaact ccgcacctcg gactattccc
     3781 ttgtgatgtc gatcatgcgc gcgatttcat atggagacac cgaggagaag tggaaaacag
     3841 agcagcagcg ggcggtgaaa tcactgctag aggaacctac agacaagaag atggctcatg
     3901 ctgaagcggc taacctgtac gagcagacga ttgccgggct tcaggagctt gtactctggc
     3961 cctggtagga catttgctgc gcctttatac attcggaaaa ctcgtgcaac aaaagtagtg
     4021 gtttctcgct ctaaataggg aggtgtttga acgaatcacc tgttgagtac aaagtcctga
     4081 ggagaacgca tatggcctgg ttcgcgagtg tcgctgcatc tgcggccgtt gtaggtgtag
     4141 ctattgggag cggcacattg ctgcttggcc atcgcttcat taaggagttt actcgtcccg
     4201 gcgtcaccat caatcgaggg acaccgcagt ggggcggctg gaccttcccc gaatcggtaa
     4261 ttgaaccccc tcctgaattg caacgttctg tgacgttcca ggcagcagac ggcgcgctct
     4321 tatgtggaga gttttgggca cagacgcgca gcgcgccgac cgtcatcatc agccatgggt
     4381 ttcacttacc ctgtgaacac ttccgctcgg tcgcagcgct cgaatatgcg catggagcca
     4441 acatcttgtt gttcgattat cgcggtcatg gaaagagttc gcagatcccg accacgtgcg
     4501 gcaacgcgga agtcaacgac ctgcttgcag ccgtggatgt cgcggcgagc caggcagaaa
     4561 ccacgcaggg ccaggtctac atccacggct tttcgatggg ggccgccgtc gcgattttgc
     4621 tgccgcccca cgctgccgtc gcaggcatca tcgccgatag ctcctatgca cgcctggacg
     4681 agatgacccg cctgctgatc ggccaggtct tcgatcagga gacatcagga tggcgcggcc
     4741 cggcgcgggt cctgcgttcc cttattccat cgctgacgcg cctgacgttt ctgggtggac
     4801 agctcctgtt tcacctacgc tttcgtcatc cactggtggc gcgacctgac caggcgatca
     4861 gcgaccatca cgtcaagcat atggtgcgcg caccagggat aatagcgccc ccgattctcc
     4921 tgattcacgc caggcatgat ccgttgatag ctctcaatca cgcccaccgc ctcgtggcac
     4981 ttgcccgcgc ggctggacgc tcgattcgag aatactacac tccctgtgac atccattgcg
     5041 gctcgtatgg atatgatccg cggcgctaca tggctctgct tttggagttt atgacactat
     5101 aatttatgcc gtcatcaagt aaaaatatga aagttcacgg cgggagtttt gataaatatt
     5161 gcacaatcgt taaaaatagt gtataattac acgataatct actccttccc atgaaccatg
     5221 gcactcacat gaaacgatac gtaaagcaca tcccctttcg ggagtggtat ggctcacatg
     5281 tcttcacatt tccatctccc caccggggag cgtgatttca ggggagatac ggcagaaaag
     5341 gatattacga tgccaccatt catactcctg ttgctcgccc tggtgttctt tgcatgcgct
     5401 ttcggctata tgatgtttcg tcacgaagtt gagaggcggg ctgcggtagc gcacgaaaag
     5461 agacggaacg aattccttga ccgcctctaa gatgaccagg aaccttcggt ttcctgcacg
     5521 aatagaacat gagatagagt atcaagcacg cactcttctc gctctcggag caaagcattc
     5581 gccacacatc cagctcgcta agaagaaagg atgtctcgac gctaaccatt gtgtgacgct
     5641 cagggtcctc ggtaaggatt tctacccatc cgtcttcggg aatggcaatt tcgcgacctg
     5701 tgagaacctg atggcggcaa cagcggcagg ggtccaaatc gctatctccg tgaagcagga
     5761 aactccaccg tttgtggacg tcagggggaa aacggcctat gatatccagg tctgtaccgg
     5821 cccgaccgca aggtacatct ggttcaaaaa ccttgatgcg tgcgccgaat tggactatgg
     5881 agacacggcc cacgaatcca gcaacttcgt gacctttacc gttgagccgc gccgcgaaac
     5941 cacagggtga ctcccatgac gatgactctc gttatcctgc ctgcctcatc agaaagttcc
     6001 tcagaataga tgcagcctac cactgatggt ctggttcatg attatgctac ctcctcgacc
     6061 tggtaccccg gcttgtccat gcgaggtccg aaaaactcga tgcggtagac gccactcgga
     6121 caggtgacct cgtgatagaa actgaactgt ggggagacca tttggacctg gctcctatta
     6181 ccctctgctg gcaatatttg gaatccctgg agtgcctcag cgctggtcgc atagtcatga
     6241 accagcccat cggcggcaat cacagtgtag tgggtggtat aaggaaactg aacacagggg
     6301 ccaaagaatg tgatggcgcc gtggttgccg ccttcgagca gcgtgacggt gtcagaagcg
     6361 aaccaggctt tccaaacggt attggaaaac gtgcccagat tggggtcaat atagcacacg
     6421 gcgtagtcgg ttcctgagac tgcctccggc ttcacactgg catttccaaa gctgatgaca
     6481 cgcatgtttt tattcgggct ccatgctctt gagtccccct gcgaatgctc ccagttgtgc
     6541 tcgccataga gcaggatggg gatgccttgt tgatagctcg cccgcgcatc tgcttgcgag
     6601 atctccttcc acgtggagag aggagcgtag aagttggtac tcttcgtcag aggtacccgc
     6661 tctgccaatt ttgatttcga ggactgcaat gagagcggcg catcgaagtc gctgagctgc
     6721 ttcatcgcga gcgtgaggtc gggaacagaa agttccttgc cactccccaa ttgtgcgtga
     6781 tacgttcttt cgagcagcca tgtttcactg agacgctcag aaaatgggac gattatcggc
     6841 aggagcgtag ccgcctgtgg tgaggttgtc ataactatga cctttcttgg gtcgagttct
     6901 tttctatcgc agtctcggtc agcattcgca ggtagcgaag ttgcagggcg gagggctgtg
     6961 cgccaaatac atcagcgtaa cctccttatg cccaaccatt taggaccaat cttttcaccg
     7021 atccgcgacg ttggatcggc tgcttttatg tcagcgatat gttgctcctg agtagccttg
     7081 cgggcgttta aacgcgcagt agcttcctgc atacgtccgg aaaatgtatc aggatactca
     7141 cttacccgct ggtatgttat caccagcttg ttcttacgac cgcgcaccag cgccagggga
     7201 accaccgcca actcggccac atttctacgt tgctgaaaag caacgctatc tacgggctgc
     7261 caaccatcct gttgctggtg ccttaagtcc ctggcatact gaccagggga cgagtacgtg
     7321 ataactttta tttgaacagc tcgttccata ggattgttaa ttcctttccg aaacatcctc
     7381 cactcttatt gcagagtcca ttcagcgcgt tagagatggc tcactactac gagaaccgtt
     7441 gagtagctcg tgggctggct cgcttgcctc gtgtcgcatc atgtggatga acggctcgat
     7501 caggtcgagg ggcagcggga agacgatggt cgaattcttt tccaccgcga tctcggtcag
     7561 cgtttgcagg tagcgcagtt gcagagcgga gggctgcgag ccgatgattt cggcagcctg
     7621 ggccagcttg gtcgaggcga gtaattcccc ttcggcatga atgactttgg cgcgcttctc
     7681 ccgctcagcc tcagcctgtt tggccatagc gcgctgcatc gtcgggggca gttccacatc
     7741 cttaatctca accgccgtca ccttgacgcc ccagcgctcc gtctgctcgt cgacaatcgc
     7801 ctgcagatcg tggttgatct ttttgcgctc ggccagcagt tcatccagtt ccgattggcc
     7861 cagcacgttg cgcagggtgg tttgaccaat ctgcgaggtg gcttgattga agttcaccac
     7921 tttgatcacc gccgcaattg ggtcgacgac gtagaagtag accactgccg tcaccctgat
     7981 cgtcacgtta tcgcgggtta ttacctcttg cggcggcacc gtaagcgtga cgatacgcag
     8041 atcgactttg gccatgcgtg agatgaaggg gggtaccagg aacagcccgg gacctttggc
     8101 cccgatcaag cgccccagca cgaagatcac accgcgctca tactgttgca ccacgcgaat
     8161 cgctgagagc gcggcgatca ccagcacaag cacgatggcg gcaacgacaa acaacacgag
     8221 tagattcata ataagcctcc ttacgacctg aaaaaacgga gaggggaaaa gctcatgctt
     8281 ctccttccct cagtcgtacg caaactccga ccccagcacg gtacgcaact gctctctcgc
     8341 ccgggaaaag tgcgttttgg ccgtcgtttc tgggatgcgg agcgcctgcc caattttacg
     8401 gtagctccat tgggtgttat agcgcagcag cacgactgcg cggtagcgcg agggcaatgt
     8461 ctcaatcgcc tcaaggagcc gctgtcgcag ctcgcgccgt tcaacctgct cctccggctg
     8521 ccgatctggg tcgggaagag tggcaagaaa tgagtattca cccccatccg ggaacggctc
     8581 gatctcagaa aagaggaggg gccgcctgcg acgcagttca tcgagagagc ggtgacgtgc
     8641 cacctggcat aaccatgcct tgagcgtccc ctctggtatc agtgtgggga gcgatcggta
     8701 caattggatg aagacatgtt gcaagacatc gtgagcgagg tgtttgtccc ccaccaggcg
     8761 gcagatcagc ctaaagagca tggcgctgta ccgctcgacc agcatttcaa acgcgccggg
     8821 atcgccgctt tgcgcttgtc ggagcaggtc gtcatccgcc agatctgaaa gccgcttgtt
     8881 tgacgcgaga tacgtttgca tcga
//
LOCUS       DASZAK010001106        10576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_703713, whole genome shotgun sequence.
ACCESSION   DASZAK010001106 DASZAK010000000
VERSION     DASZAK010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703713"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1241
                     /locus_tag="VGT44_19560"
     CDS             54..1241
                     /locus_tag="VGT44_19560"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2583067.1"
                     /translation="MINEIQDLVIIGGGVMGLCTAYAAAPFGGKITMLERSRIGDKQT
                     ASFGYTRSIRNDYLDPQYARLAYEARRLWLQIEQHAEEPILINCGCLNIARESITPDL
                     DASYAEQSYRVLSDLHLKTEIFTREELRERYPQFDADLGRLDVEAGFVYVPAATQTLL
                     AALNERHVRIEEEVEVVAIERLQGKIVVYTGDGSQFATRKLVITAGHGTNDVLARIEG
                     CRTRFPISPDRPSQCRYFLPPAERLEEFVAGALPVFAYLDVGIYGHPIYAGKTPGVKI
                     GYYNPPDVRVACTRIHDIASFIEECMPSLRGVASVDVTDVDQCNYDLVADDNFILGAL
                     PDMPDIAVGVGWRGTGYKYAPWVGQTLMQLAFQQGSVYDISRFAPGRFADSADTEITR
                     RKS"
     gene            1238..2275
                     /locus_tag="VGT44_19565"
     CDS             1238..2275
                     /locus_tag="VGT44_19565"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEV2583068.1"
                     /translation="MNNRTSRGILERLREGVVMGAEGYVFELERRGYIKAGPYVPEVV
                     LDFPEAVRELHREFLRAGSDVMVALTYYAHREKLKDVGREGDLERMNRQAVRLANEAA
                     REGDALVAGNICNTWAYDPANVRESSRVVRAMYEEQLGWAVEEGIDFVISETNDYLGE
                     ALIALEVIKQLGLPAMVTLASVQPGETRDGYDYVEACKRLADAGADVVGLNCDRGPVT
                     MLPIIERVRAAVGCYVATQPVPYKTDAQHLTFESLRTAGEEQAFPLALEPHLCNRFEM
                     ADFARRARAIGVNYIGICCGGAPHYVRAMAEALGKTTPASKYSPAMELHPMLGEQVEE
                     KDQHFLADWKS"
     gene            2277..3167
                     /locus_tag="VGT44_19570"
     CDS             2277..3167
                     /locus_tag="VGT44_19570"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEV2583069.1"
                     /translation="MMLRFATYLAPILYDTYQSIARYIGEQVGAATTLVVGQSLHDFA
                     VGKVDVGFTCGLPYALMVATYAKRDAPACPIELLAAPVLLGERYRGRPIYFSDVVVRS
                     DSPYASFDDLQGCRWAYNQPESHSGWNLVCYNLLKRGKTPAFFGQLIQSGSHQRSLEM
                     VLAGEANAAAIDSHVLDVFLARDKEAAASLRVIDMFGPSSIPPIVVAIALDLALKRRV
                     QDALLAMHEDPCAAGVLHEGAIARFARVRDGEYDDLREMHRVAKGNVGAQFIAPSSQA
                     DARHGGRNELRPYISPFNQV"
     gene            3200..4501
                     /locus_tag="VGT44_19575"
     CDS             3200..4501
                     /locus_tag="VGT44_19575"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEV2583070.1"
                     /translation="MATSSLKTDDQAVQTTLSLLQDVFGSVQLSNFSVRLWDGTAWQS
                     RPEAGEAPSFTLVLQHPGALRAMLLPPSELNLGEAYIYNDYDVEGDLAGAFEMGDFLM
                     DERWGKMAQARYGKRLLRLPRTGQARPRDTAAKLHGSLHSKERDRQAVTYHYDRSNDF
                     YALWLDRRMVYSCAYFAAPDDDLDTAQERKLDYICRKLRLRPGERLLDIGCGWGGLVT
                     YAAQRYGVETYGITLSQPQAELAQQRIQEAGLADRCRVEVRDYRDVQENGAYDKIVSV
                     GMFEHVGETLLPAYFRQAWQLLRPGGVFLNHGIASVITNPPQRESAFNQRYVFPDGAL
                     VPISTTLRVAEASGFEVRDVESLREHYALTLRHWVRRLESHAEEARRYTNDVTYRIWR
                     VYMSGSIHAFQTGAANIYQTLLSKPERGKSRLPLTRADWYA"
     gene            complement(4515..5276)
                     /locus_tag="VGT44_19580"
     CDS             complement(4515..5276)
                     /locus_tag="VGT44_19580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEV2583071.1"
                     /translation="MTLQRERNAILALLLILAAVAWALLVWQSTMLSPMSMGLTMGMG
                     AVLFLAIWVVMMVAMMFPTAAPMILMFARVHRDKRAGGKAFVPTWVFVSAYLLLWTLF
                     GVLAYVGAVEAGRLAQQVPWLAAHAPQIGGGVLALAGLYQLSPLKRSCLAKCRTPLDF
                     VLGSWRDGYLGSFRMGLEHGVYCLGCCWLLFVIFFPLGMMNVAAMAVVTLLIFAEKSF
                     PLGQRIAQLAAVALIIYGAVVVFVPGALPLGQTGM"
     gene            complement(5281..5583)
                     /gene="nrtS"
                     /locus_tag="VGT44_19585"
     CDS             complement(5281..5583)
                     /gene="nrtS"
                     /locus_tag="VGT44_19585"
                     /inference="COORDINATES: protein motif:HMM:NF038050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NrtS"
                     /protein_id="HEV2583072.1"
                     /translation="MSAESTQPGNCTRCEQSLADRAAYLIDGAYRCLRCTLQYRPLLR
                     RSAQTSLVVGTLLVAINQGAVLLQGHISPALLWEIPLTYLVPFCVSTWGALTNTRR"
     gene            complement(5573..6199)
                     /locus_tag="VGT44_19590"
     CDS             complement(5573..6199)
                     /locus_tag="VGT44_19590"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEV2583073.1"
                     /translation="MADWKMKGQYLKNCSCLASCPCDTIGVPAPHKFCEGVVGMNIQE
                     GHFDNVDLSGLKWAANVHWPGALHEGNGTIEVFVDESASEAQRNALLTILTGQAGGTL
                     FEILSQIVTTVHGPHFVKIGWEFDKAKRRARLVIPGFVETTSEPLSVPATGEEQRVIV
                     RMPNGFEYREMEVAQSAVLKGTGEIKFDWKGTHSSLAEVEHTPAGLVG"
     gene            6550..10047
                     /locus_tag="VGT44_19595"
     CDS             6550..10047
                     /locus_tag="VGT44_19595"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV2583074.1"
                     /translation="MKKAARAIPNRQLRQERELRCWSQLEVADQIGTTSFNVSRWERG
                     ITFPTAHFRQELCALFGKSAVELGFLQDDDSHKSLLPHSLPTGQDVVSLPPQTARIAE
                     ERKLVTFLLADMTESTELGETLDPEDLHALMGRYYAHARRVIESYGGTLEPLIGSTMT
                     AAFGLPHAHSDDAERALAAALRLREAVAIDSLPGGNLLLRTGISTGVVIATNNPSGGA
                     FLLTGDAVNTAARLQQAASFGEILVSERTAAATQAAFLFHDARLVEVKGKRHPLRAFP
                     LWQARTLRQVERPALVGRQPDLRQLELLGERALMERRPQLVSIVAPAGTGKTRLLEEF
                     LAHLELAEGFQVAVARCPPYGQTLIYWPLRGLLSGLLGEASGKPPVVEAFVRGGQTLE
                     DASRLADLVLTTLGVEQEEVADRESIFAAWRLLLESLALKAPRIVVFEDLHWASESLL
                     DLVEYIMHLHVQSSLLVIVLSRPELLDRRPTWGGGQQNFTAVTLQPLSEVQTRELVRH
                     VAMELNEALQERVVDRSGGNPFFALELIHGLVENSATVGVLPDTVHAAVLARLDMLSS
                     HERMIAQAASVAGRVFRVAALRAVLEGLTTSEFDRALDGLMTRHLVAQTSGWGFTFQH
                     VLIRDVAYGSLSRSERVRLHSAIAAWFEAFEAGGLDEFTELIAYHYREAVKLTRQSAI
                     PLPVDLTRVVRALERASLLASRSGALAEARAYLQSAIELAEEREHLRLYEQLGDALLQ
                     GHSAVDAYRKAIESWRGTADQDPLVGGRLLRKLLMAYTRWNAWDVQARPTQEELNEVL
                     IEAQRLAETSGDEDERWRVRLARIRLLVWSGNSTLQEAEEGRGLSLAIAAYFEERNDL
                     ISYREALNGYIALSYRVDADHDALEASQLRLSISDLPLVERADALQVMAATLLNLGNY
                     SRCIEVVREALTQLRPGEPVVHFDAAIAFAMWALLYGGRWSEISDFRSALENIWEQIQ
                     HGVGANTHVAGSYLCLLHIALARENSSEVDAAISVLEQCFSSEQVNARALLAAYREDD
                     PRHLNFESTSDEWTIPMLMFFNDRGISAPRALIARLRALISTLPIDSLIRIVEIAEAL
                     EHEDDVRLSAAIEGAEAHGAIAHAARMRLVLARRTGDLTQLDRTRPILEQLGDRQFLR
                     RLARATNELVTKADPRHTF"
     gene            10063..>10576
                     /locus_tag="VGT44_19600"
     CDS             10063..>10576
                     /locus_tag="VGT44_19600"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2583075.1"
                     /translation="MRGRYPEEEGFIERDGVRIFYEVFGAGEPTVLLLPTWSVVHSRI
                     WKAQVPYLARHCRVITFDGRGNGKSSRPATPDSYTDREFAEDALAVMDATATSQAVLL
                     SLSAGTYWSLLLAAEHPERVAGAIYIGVSYPSLPNHPERMVYSFDDVLDTDEGWAKDN
                     RHYWLKNYRSF"
BASE COUNT         2161 a         2988 c         3261 g         2166 t
ORIGIN      
        1 atccggcata cgttacaaaa ccatgtttga gccgcaattg aaggaaggaa tacatgataa
       61 atgaaataca ggacctggtg ataattggcg gcggcgtgat gggattatgc accgcttatg
      121 ccgccgcgcc ctttggcggc aagatcacca tgctcgaacg gtcgcgtatc ggcgataagc
      181 agactgcttc gtttggctat acacgctcga ttcgcaacga ctacctcgat ccgcagtatg
      241 cgcggctggc ctatgaagcg cgtcgactgt ggctacagat cgaacaacat gccgaggagc
      301 caatcctcat caactgcggc tgcctcaata tcgccaggga gagcatcact cccgacctgg
      361 acgcgagcta tgccgagcaa agttatcgcg tcctcagcga cttgcatctc aaaacggaaa
      421 tcttcacgcg tgaggagcta cgagaacgct acccgcagtt cgatgctgac ctggggcgat
      481 tggatgtgga ggcgggtttt gtctacgtgc ctgccgcgac gcagacactg ctcgccgcgc
      541 tgaacgaacg gcatgtacgc attgaagagg aagtcgaggt tgttgccata gagcggctac
      601 aggggaagat cgttgtgtat accggcgatg ggagccagtt tgcgacgcgc aagctggtca
      661 tcacagcggg acatggcacg aacgacgtgc ttgcgcgcat cgaaggctgc cgcacacgtt
      721 tccccatcag ccctgacaga cccagccagt gccgctattt cctgccgccc gcggagcggt
      781 tggaagagtt tgtggcgggc gcgctgcccg tttttgctta cctggatgtc ggtatatacg
      841 gccatccgat ctatgccggg aaaacgccgg gcgtcaagat cggctattac aatccgccgg
      901 acgtgcgcgt cgcctgcacg cgcatccacg acatcgccag ctttatcgag gaatgtatgc
      961 cctcgctgcg cggcgtcgcg tcggtcgatg tgacggatgt cgatcaatgc aactacgatc
     1021 tcgtggccga cgataacttt atcctcggcg cgctgcccga catgcccgac atcgccgttg
     1081 gtgtgggctg gcgcggcacc ggctataaat acgccccctg ggtaggtcag acgttgatgc
     1141 agctcgcctt ccagcagggc agcgtatacg acatcagccg ctttgcgccg ggcaggttcg
     1201 cggatagcgc ggacacagaa ataacaagga ggaaatcatg aacaatagaa catccagggg
     1261 tatcctggaa cggctgcgcg agggtgtggt gatgggagcc gagggatatg tcttcgagct
     1321 tgagcgcagg ggctatatca aggccgggcc gtatgtgccg gaggtggtgc tggatttccc
     1381 ggaggcggtc agagaattgc accgcgaatt cttgcgggcg ggcagcgatg tgatggtggc
     1441 gctgacctac tatgcccatc gtgaaaagct gaaggatgtt ggcagggaag gcgacctgga
     1501 acgcatgaat cgccaggcgg tgcggctggc caacgaggcc gcgcgcgagg gtgacgcgct
     1561 ggtggcgggc aatatctgca atacgtgggc ctacgatccc gccaacgtca gggaatcctc
     1621 gcgcgttgtg cgggcgatgt atgaagagca actgggctgg gcggtagagg aaggcatcga
     1681 tttcgtcatc tccgagacga atgattacct gggagaagcg ctgatcgccc tggaggtcat
     1741 caagcagctt ggcttgcccg cgatggtaac gctggcctct gtgcagccgg gcgagacacg
     1801 cgatgggtac gactacgtcg aggcgtgcaa gcggctggcc gacgcggggg cggatgtcgt
     1861 cggcctgaat tgcgaccggg ggccggtaac gatgctgcct attatcgaac gggtacgcgc
     1921 cgccgttggc tgttacgtgg ccacccaacc cgttccctat aaaaccgacg cacagcattt
     1981 aaccttcgag tcactgcgca ccgcaggcga agaacaggca ttccccctgg cgctcgaacc
     2041 gcatttatgc aaccgtttcg agatggccga ttttgccagg agagccaggg ctatcggcgt
     2101 caactacatc ggcatctgct gcggcggcgc gccccactac gtgcgcgcca tggcggaggc
     2161 gctgggcaaa accacgcccg ccagcaagta ttcgcccgcg atggaactgc accccatgct
     2221 gggagagcag gttgaagaaa aagaccagca ttttctggcc gattggaaga gttgagatga
     2281 tgctgagatt cgcgacgtat cttgctccca tcctttacga cacctaccag tccatcgcgc
     2341 gctacatcgg ggaacaagtg ggcgcggcca ctacgttagt cgttggacaa tcgctgcatg
     2401 acttcgccgt aggcaaggtt gatgtaggat tcacctgcgg tctgccctat gccctgatgg
     2461 tggctaccta tgcaaagagg gacgcgcccg catgtcccat cgaactgctt gctgctcccg
     2521 tcctgctggg agagcgttac cggggcaggc ccatctactt ctccgatgtc gtggtacgca
     2581 gcgatagccc ctatgcctcg ttcgacgatc tacagggatg cagatgggcc tacaaccagc
     2641 cggaatcgca ctccggttgg aacctggtct gctacaacct gctcaaacgc ggcaaaacac
     2701 ccgccttctt cggccagttg attcaatccg gttcgcacca gcgttcgctc gaaatggtgc
     2761 tggcaggcga ggccaatgca gccgcgatcg attcgcacgt actggatgtg tttctcgcca
     2821 gggacaaaga ggcggcggca tccctgcgcg ttatcgacat gttcggcccg tcaagtatcc
     2881 cgcccatcgt ggttgctata gcgctggact tggccctgaa gcgccgcgtg caggatgcgc
     2941 ttttggcgat gcacgaagac ccgtgcgctg ccggggtgct acacgaggga gcaatcgccc
     3001 gcttcgcgag ggtgcgcgac ggggaatatg atgatttgcg cgagatgcac agggtggcga
     3061 agggaaatgt aggggcgcaa ttcattgcgc cctcttccca ggccgatgca cgacatggtg
     3121 ggcgcaatga attgcgcccc tacatttctc cattcaatca agtgtgatag gccacttacg
     3181 agcaggaagg agcaatcgca tggctacatc gtcgttgaag acagacgacc aggccgttca
     3241 aactacattg tcgttgttgc aggacgtttt tgggagtgtt cagctgtcca atttcagtgt
     3301 gcggttgtgg gatggcactg catggcagtc caggccggaa gcgggagaag cgccgagctt
     3361 tacgctggta ttgcagcatc ctggcgcgtt gcgcgctatg ttgttgccgc cgagcgagct
     3421 gaacctgggc gaagcatata tctacaacga ttacgatgtt gagggagacc tggcgggcgc
     3481 ttttgagatg ggagattttc taatggatga gcgctggggg aagatggcgc aggcgcgcta
     3541 tggtaaacgc ctgttgaggc tgcccaggac gggtcaggcg cgcccgcgcg acacggctgc
     3601 gaaactgcat ggctcgctgc actcgaagga gcgcgaccgg caggccgtga cctatcatta
     3661 cgatcgctcc aacgactttt acgcactctg gctagaccgg cgcatggtct actcgtgcgc
     3721 gtatttcgcc gcgcccgatg atgaccttga cacggcgcag gaacgcaagc tggattacat
     3781 ctgccgcaaa ttacgcttgc gtccggggga acggctgctc gacatcggct gcggctgggg
     3841 tgggctggtg acgtatgccg cacagcgcta tggcgttgaa acttatggca ttacgcttag
     3901 ccagccgcag gccgaactgg cgcagcagcg catccaggag gcgggattag cggatcgctg
     3961 ccgcgtggag gtacgcgatt accgggatgt acaggagaac ggtgcttacg acaagattgt
     4021 cagtgtgggc atgtttgagc atgtaggaga gacgcttttg cctgcctatt ttcggcaggc
     4081 atggcagcta ctacgtcctg gtggtgtctt cctcaatcat ggtatcgcga gtgtcataac
     4141 caatccgccg cagcgcgagt cggcgttcaa tcaacgctat gtcttccccg atggcgcgct
     4201 tgttcccatc agcacgacgc tgcgcgtagc cgaggcgagc ggtttcgagg tgcgtgatgt
     4261 ggagagcctg cgcgagcatt acgctctcac actgcgacac tgggtgcgta ggctcgagtc
     4321 tcacgcggag gaggcgcgcc gatataccaa cgatgtcacc tatcgcatct ggcgtgtgta
     4381 tatgtccggc tcgatccacg catttcagac cggggcagca aatatctatc agacgctgct
     4441 ctcgaagcca gagcggggga agagccgttt gcccctgacg cgcgctgatt ggtatgcatg
     4501 aagggctgct gtcgttacat ccctgtttgt cctagcggga gcgcccccgg gacgaacacg
     4561 acaacagccc cgtaaatgat cagcgctaca gcggctagtt gagcaatgcg ctgccccagc
     4621 ggaaaggact tctcggcgaa gatgagcagg gtaacgacgg ccatcgctgc tacattcatc
     4681 atgccgagcg ggaagaagat cacgaagagc agccagcagc agccaagaca gtaaactcca
     4741 tgctcaagcc ccatgcggaa agatcccagg tatccgtcac gccaggaacc gagaacgaag
     4801 tctagaggtg tacggcattt cgccaggcag gaacgcttca gtggggagag ctgatagagt
     4861 ccggccagcg cgagtacgcc cccgccgatc tgaggggcat gagctgccaa ccacggcacc
     4921 tgctgcgcca gcctccctgc ctcgaccgcg ccgacatagg ccagtacgcc gaagagggtc
     4981 cagagcagca ggtacgcgct gacaaacacc caggttggga caaaagcttt cccacccgca
     5041 cgcttatcgc gatggacgcg ggcgaacatc aggatcatcg gtgctgcggt aggaaacatc
     5101 atagccacca tcatgaccac ccagatcgcc aggaacaaca cggctcccat gcccatggtc
     5161 aggcccatgc tcataggaga cagcatggtg gattgccaga cgaggagtgc ccaggcgaca
     5221 gcagccagga tcagcagcaa ggccaggatg gcgtttcgct cgcgttgcag cgtcatacga
     5281 ttacctccgg gtattggtga gcgcgcccca ggtagagacg cagaacggca caagataagt
     5341 gagcggaatc tcccacagca gcgccgggga gatgtgtcct tgcagcagga cggcgccctg
     5401 attgatagca accagcagcg ttcccaccac cagcgatgtc tgcgctgaac gccgcaggag
     5461 cggtcgatac tgcaacgtgc agcgcagaca tcgatacgct ccgtcaatca ggtacgccgc
     5521 acggtccgcc agggactgct cgcaacgtgt gcagttccct ggctgcgtgg actcagccga
     5581 caaggccagc cggggtatgc tcgacttcgg ccagcgagct atgagtccct ttccagtcaa
     5641 acttgatctc gccggtcccc ttaagtacag ccgactgcgc gacttccatc tctcggtact
     5701 cgaagccatt cggcatgcgc acgatgaccc gctgctcttc gcctgttgcc ggtacgctga
     5761 gcggttcgct ggtcgtctct acaaagccgg gaatgacgag tcgagcccgt cgcttcgctt
     5821 tgtcgaattc ccagccaatc ttgacgaaat gtgggccatg aacggtggtc acgatctgcg
     5881 acaggatctc aaagagcgtg cctcctgcct gacccgtcag gatcgtgagc agggcattgc
     5941 gctgcgcctc gctagcgctc tcgtccacaa agacctctat cgtgccattg ccctcgtgga
     6001 gggctccagg ccagtgaaca ttcgccgccc acttgagccc gctcaggtcg acgttgtcga
     6061 agtgaccttc ctggatgttc atcccgacga cgccctcaca gaacttgtgg ggtgctggaa
     6121 cgccaatggt gtcgcaagga caggacgcca ggcaactgca gttcttgaga tactgccctt
     6181 tcattttcca atccgccatg ttaaatctcc cttctgaata ctttgtttcg cttgcctttt
     6241 gataaaggct tcttgctcat gatgcttcta gtatgcctgg taaggcggct ggtagggaat
     6301 ggcatcaact ggtcaagtaa tagtcaagta gtagcacaca ggattcgttg tctcgatggg
     6361 gagaatctga tacaatgacc tcaccaatcg tttacttcaa cgatagtgac tcactttttg
     6421 atgcagtaag catcgtttag tgtagcagcc gcgtgaaccg cgattgctca catgtgaatt
     6481 ggatgttcgt cgtgccagaa cctatggggg agaagagtag agagggacaa agaagggagg
     6541 gagccagagg tgaaaaaggc tgctcgtgcg atcccgaatc ggcaattgag acaggagcgt
     6601 gaactgcgct gctggtcgca gttagaagta gcagatcaga taggcactac gtctttcaac
     6661 gtgagtcgat gggagcgcgg cattacattt cccactgctc acttcaggca ggaactctgc
     6721 gcgctttttg gcaagagcgc cgtggaactc ggctttctgc aagacgatga cagtcacaag
     6781 tcccttcttc cccattcact gccgactggc caggacgtgg tctctctccc tccacaaacg
     6841 gcgcgcatag cggaagagcg caaactcgtg acgttcctcc tggccgatat gaccgagtca
     6901 acggaactgg gagagacact ggatccagag gatttgcatg ccttgatggg tcgctactac
     6961 gcccatgccc gccgtgtcat tgaaagctat ggcggcactc tggagccgtt gattggaagt
     7021 actatgacgg ctgcctttgg gttgccgcat gcccatagcg atgatgctga gcgagctttg
     7081 gccgccgccc tgaggctgcg tgaggcggtg gcaatcgact cgttgccagg cggaaacttg
     7141 ttgctgcgca cggggattag tacgggtgtg gtcatagcca caaacaatcc ctccggcggc
     7201 gctttcctgt taaccgggga tgcggtcaac accgcggccc ggctgcaaca ggccgccagt
     7261 tttggcgaaa ttctcgtcag cgagcggacc gcggctgcga cccaggcagc ctttctcttc
     7321 catgacgcgc gactggtcga ggtcaagggt aaacgccatc ccctgcgcgc ctttcccctc
     7381 tggcaggcgc gtacgctgcg gcaggtcgag cggccggcgc tggtgggtcg ccagccggac
     7441 ttgcgccagc tggagctgct gggggagcgc gccctgatgg agcggcgacc gcaactggtc
     7501 tcgattgtgg cgccagcagg tacgggcaag acgcgcctcc tggaggagtt tctggctcac
     7561 ctcgaactgg cagagggatt ccaggtcgcg gtggcgcgct gcccgcccta cgggcagacg
     7621 ttgatctact ggccgctgcg gggcctgctg agcggcctgc tgggcgaagc aagcggcaaa
     7681 ccaccggtcg tcgaggcatt tgtacgagga ggacagacgc tcgaggatgc ttcacgcctg
     7741 gccgatcttg tgctgaccac gctgggcgta gaacaggagg aggttgctga ccgcgagagt
     7801 atcttcgccg cctggcgact gctgcttgag tccctcgcac tcaaggcgcc acgtattgtg
     7861 gtttttgaag atctgcattg ggcaagcgag agtctgctcg acctggtcga gtatatcatg
     7921 catctccacg tgcaatcctc cctgttggtg atcgttctga gcagaccgga gttgctggat
     7981 cgccgtccca cctggggtgg aggacagcag aacttcaccg cagttaccct gcaaccgctc
     8041 tccgaggtcc agacgcggga actggtcagg cacgtggcga tggaactgaa tgaggcactc
     8101 caggagcgcg tcgtggatcg ctccgggggc aatcccttct ttgcccttga actgatacac
     8161 ggcttagtgg agaacagcgc cacagtcggc gtgctgccgg atacggttca cgcggcggta
     8221 ctcgcccgtc ttgatatgct ctcgtcacac gagcgaatga ttgcgcaagc ggcttccgtc
     8281 gccggtcgag tttttcgcgt tgcggcgctg cgggcagtcc tggaagggct gaccacgagt
     8341 gagtttgaca gagcgctcga tggcttgatg acacgccatc ttgttgcgca gacttcgggc
     8401 tggggtttta cattccagca cgtactcatt cgtgacgtgg cctatgggtc gctctcccgc
     8461 tctgaacggg tgcggctaca tagcgcgatt gcagcgtggt ttgaagcgtt cgaagccggg
     8521 gggctggacg agttcaccga actgatcgcc tatcactatc gagaggccgt gaagctcacg
     8581 cggcaatcgg caatccccct gcctgtcgat cttacccgtg tcgtccgcgc gcttgagcgg
     8641 gcgagcctgc ttgccagccg ttccggcgca ttggccgagg cacgcgccta tcttcaaagc
     8701 gccattgagc ttgcggagga gcgggagcat ttgcggctct atgagcaact tggcgacgct
     8761 ctgctccagg gtcacagcgc cgttgatgcg tatcgtaagg ccattgaaag ctggcgtggg
     8821 acggcggatc aagatccatt ggtcggagga agattactcc gtaagttact gatggcctac
     8881 acccgttgga acgcctggga tgtccaggcg cgacccacac aagaggaact gaatgaagta
     8941 ctgatcgaag cgcaaaggct cgcggagacg tcgggtgatg aggacgagcg ctggcgcgtg
     9001 cgcctcgcaa ggatcagatt gctcgtctgg agcgggaata gcactctgca agaagccgag
     9061 gaaggacgtg gtctctctct cgcaatagcg gcatatttcg aggaacgaaa cgatttgatc
     9121 tcttacaggg aggcactgaa tggatacatt gcgctgtcgt atcgtgttga cgcggatcac
     9181 gatgcgcttg aggcatcaca actccgcctg agtatttctg atctgccact ggttgagcgc
     9241 gccgatgctt tgcaggtgat ggccgcgact ctcctcaacc tcggcaacta ttcccgctgt
     9301 atcgaggttg tgcgcgaggc gttgacgcag cttcgacctg gcgagccggt ggtgcatttt
     9361 gatgcggcaa ttgcctttgc aatgtgggcg ctcctctacg gtggacgctg gtctgagatc
     9421 agtgacttca gatccgcgct tgaaaatatc tgggaacaga tccagcacgg tgttggcgct
     9481 aatacacatg tggccggcag ctatctctgc ctgcttcata ttgcactggc gcgcgagaac
     9541 tccagcgagg ttgacgctgc tatctcggtg ctggaacagt gtttctccag cgaacaggtc
     9601 aacgcacgcg ccttgcttgc agcctaccgc gaggacgacc cacgccatct caactttgag
     9661 tcgacgagtg atgagtggac tatccccatg ctgatgttct tcaacgatcg tggaatatcc
     9721 gcaccgcgag cgcttattgc gcgacttcgc gctttgatat ccaccttacc gatcgattcg
     9781 ttgatccgta tcgttgagat cgccgaagcg cttgaacacg aggacgatgt acggctgtca
     9841 gcagccatcg agggggcgga agctcatggc gcgattgcgc acgcagcacg tatgcgcctt
     9901 gtactcgctc ggaggacggg cgatcttaca caactcgacc gtacccgacc gatactggag
     9961 cagctcggag accgccaatt cctccgccgg ctagcgcggg ctacaaatga gcttgttaca
    10021 aaagccgatc cgcggcacac attttgaagg agggaacgat gcatgagggg gcgttatcct
    10081 gaggaagagg gctttatcga gcgcgacggc gtccgcatct tttatgaagt ctttggagcg
    10141 ggtgaaccca cggtacttct cctccccacg tggtcggttg ttcactcgcg catctggaag
    10201 gcacaggttc catatctcgc ccgccactgc cgcgtcataa ccttcgatgg gcgtggcaac
    10261 gggaagtcga gtcgccctgc gactcccgat tcctataccg atagggagtt tgcggaggac
    10321 gcgctggcgg tgatggatgc gacggctacc tctcaggccg tactcctctc gttgtcggct
    10381 ggcacgtact ggtcactgct cctggctgct gagcatcctg agcgagtcgc aggggcaatc
    10441 tatattggag tgtcataccc ttctcttcca aatcatccag agcggatggt ctattcgttc
    10501 gatgatgttc tcgataccga cgagggttgg gcaaaggata atcgccatta ctggctcaag
    10561 aactaccgga gctttc
//
LOCUS       DASZAK010001107         4546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_596980, whole genome shotgun sequence.
ACCESSION   DASZAK010001107 DASZAK010000000
VERSION     DASZAK010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_596980"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="VGT44_19605"
     CDS             <1..435
                     /locus_tag="VGT44_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583076.1"
                     /translation="DDYLTKPFSIDELLARVRAVLRRAQFSVRENTQGMQSTLTTGDL
                     TIDYTQHLVMLAGQEIALTPTEYRMLAYLAQNAGHIVTQDLLLEHVWGAEYLGESHML
                     QVNINRLRHKIETDPTLPRYILTKVGVGYSLAALPPTAMATG"
     gene            complement(468..704)
                     /locus_tag="VGT44_19610"
     CDS             complement(468..704)
                     /locus_tag="VGT44_19610"
                     /inference="COORDINATES: protein motif:HMM:NF021520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HEV2583077.1"
                     /translation="MTHSTAMPRRPVCSLWKRRGELFVRIMEAAASIKPGETLVIIAP
                     FEPVPLYDVLGVRGYLHDTASIAVDEWVVRFSRA"
     gene            785..1456
                     /locus_tag="VGT44_19615"
     CDS             785..1456
                     /locus_tag="VGT44_19615"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEV2583078.1"
                     /translation="MSIDVEELRHIPHFATLSGEELAHVAAMTVERHYERGDLILLEG
                     DTGGALHYVRSGLVKVYKTSSAGKDQVLRLIAGGNTFNDVPALDGGPNPASAAALEPS
                     AIYVIQRAELRKLILTRPEVAEAVVKTLANALRHLVSLVEDLSLRHVTARVAKILLDQ
                     EASTLEGQQVYRLTQQEMAALAGTAREVVGRALKELEAAGAIEMRQGRAVVMNPERLR
                     MLSSG"
     gene            1624..2781
                     /locus_tag="VGT44_19620"
     CDS             1624..2781
                     /locus_tag="VGT44_19620"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEV2583079.1"
                     /translation="MSKQLLEPGQRPEEPSTAQTTERTATQATNGTYAPAPVRVPYAE
                     PRRKPWGLTFMTASVLAAVLILALALVGVAALANRSNAQSPASSSTQTAASNSTPVPQ
                     IAPPAHQLYNAQAPSTPQGNTVNLTLTVKEVLVSIAPGVAYHAWTFNGTVPGPVLRVR
                     QGQTIHFTLVNDGTMPHSIDFHAAQTPWNVNYQEVAPGKSFSFDWKANYPGVFMYHCG
                     APTVIYHMANGMYGAIIVDPATGWVPAKEFVLVQSEFYTTQLPDGSYFVDPNKLSAGN
                     PDYVVFNGYANQYKTSPLVARAGERIRIFIVNAGPTLFSAFHVIGAIFSDTYADGNPA
                     NHMVGNQTVTVPPGGAMMVELTIPQAGLYPFVTHSFADVGKGAIGILKITP"
     gene            2904..4328
                     /locus_tag="VGT44_19625"
     CDS             2904..4328
                     /locus_tag="VGT44_19625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583080.1"
                     /translation="MPTTAYEKIRPLPMPEGVHKQQPFSRVAPILKTAMLMGVGGGFL
                     LATILTVTYTLSLPLGTWWEALAQAHGHLQLYGWAGLFVLGVALHFLPRLRGAPLVGA
                     RLVPWLLGILIASLVLRAISQPLLSVSPSAAWQIGLLVSSGVLEVVAYAVILALLVLT
                     ALSGPRPTTRPAYWSVLPFLAGAFCSLGIASIVNLLNVIQAAQESGLAPYTGDTLNVT
                     LGLFGFLVPVALAMSARSLPMYAGLDGFPRRVLWPLAGAYFAGVILLCLGSGGPLAAP
                     WVSIIDGLGMALLGGVVLLFVGIFLSLMRRRGRLPERVAKLAPSPETLAQTYQKQVKQ
                     EQTHYGPFVGLVASAYLWAMLGALLLLIDGGSVLATGSELVAFDAIRHAFAIGFLALL
                     ICGIAPRMLPSFSGGRIASPKLVSATLWLGNAAAVLRVGALLFAPLFAGTQGFTFDTI
                     LFGLSGPFGLAMAICLAINLWPAL"
BASE COUNT          925 a         1422 c         1300 g          899 t
ORIGIN      
        1 gatgactacc tgaccaaacc attcagtatc gatgaattac tggcgcgagt tcgcgctgtc
       61 ctgcgccggg cgcaattctc ggtcagggaa aacacccagg ggatgcaatc gacgctgaca
      121 acgggcgacc tgacgatcga ctacacgcag cacctggtca tgctggcagg gcaagaaatc
      181 gcactcacgc ccacggaata tcgcatgctg gcctacctcg cgcagaatgc cggacatatt
      241 gtgacccagg atctgttgct tgagcatgtc tggggggcag aatatcttgg agagagtcac
      301 atgctgcaag tcaacatcaa ccgcttgcgc cacaagatcg aaaccgaccc cacactcccg
      361 cgttacatcc tgaccaaagt aggtgtgggc tattcgctgg ccgctctgcc cccaactgca
      421 atggcaaccg gctaacaatc tattcgaatt gcaaacgctc tggtatctta cgcacgtgaa
      481 aaccgcacta cccactcgtc gacagcaatg ctggctgtgt cgtgtaagta cccacgcaca
      541 cccaggacgt cataaagcgg aaccggttca aagggggcga taatcaccag cgtttcgccg
      601 ggcttgatgg aagccgctgc ctccatgatg cgtacaaaca gttcacctct tcgcttccac
      661 aatgagcaga ctggccgcct gggcatggct gttgagtgag tcatcagatt gacacacaag
      721 tccttgctct gctattgttc tctcatagca gcggactatc ccattggcgg gaaggaggag
      781 ccttatgtcg atcgatgtcg aagagctgcg tcatatccct cactttgcta ccctgagcgg
      841 agaggaactg gcgcatgtgg cagccatgac ggtggaacgg cactacgagc gtggcgatct
      901 catcctgtta gagggcgaca cgggcggagc attgcattac gtgcgctcag ggctggtcaa
      961 agtatacaag acgtcgtcgg cgggcaaaga ccaggtgctt cgactcatcg cgggaggcaa
     1021 caccttcaac gatgtgccgg ccctcgatgg cggtcctaac cccgcaagcg cggcggcctt
     1081 agagccaagc gccatctacg tcattcagcg cgccgaactg cgtaagctca tcctgacgcg
     1141 gccagaggta gcagaagcag tggtcaagac gttggcgaat gcgctgcgtc acctcgtttc
     1201 cctggtagaa gatctctcgc tccgccatgt cactgcccgc gtggcaaaaa tactcctcga
     1261 ccaggaagcc tccacccttg aagggcaaca agtctatcgt ctgacccagc aagaaatggc
     1321 agcgctggcc ggcaccgccc gcgaagtcgt gggtcgcgcg ctcaaagagc tggaagcggc
     1381 gggcgctatc gaaatgcgcc agggtcgtgc tgtggtaatg aaccccgagc gtctccgcat
     1441 gctcagttcg gggtaaagaa gcgcttcctc gctacactca ttgcgccgta tcacaatcac
     1501 ctcacaacgc agcatgtgtg acaaaagtcg cttcgcaagc gagcgcgaat accgtattgt
     1561 tttgctcaga gacgaaatca attctgaatt catcctcgat cagagaggag agggagaaat
     1621 cctatgagca aacaactgtt agagcctggg cagcgtcctg aagagccatc aacggctcag
     1681 acaaccgaac gaaccgcgac ccaggcgaca aatggcacat acgcgcccgc tccggtacgt
     1741 gttccctatg cagaaccgcg ccggaaaccc tgggggctga cattcatgac ggcctcggtc
     1801 ctagccgcgg tgttgatcct ggcgctcgct ctcgtcggag tggcggcact cgccaataga
     1861 agcaacgccc agtcacccgc aagcagcagc acccagaccg ccgccagcaa tagtaccccg
     1921 gtcccgcaaa ttgcgccgcc cgcgcaccag ctctacaacg cacaggcccc atccacgccg
     1981 caaggcaata ccgtcaattt gacgctgacc gtcaaagagg tgcttgtttc catcgcgccg
     2041 ggggtagcct accacgcctg gacgttcaac ggcacggtcc ccggcccggt gctgcgcgtg
     2101 cgccaggggc agaccattca tttcacgctg gtgaatgatg gcacgatgcc gcactccatc
     2161 gacttccatg ccgcgcagac accctggaac gtcaactacc aggaagtcgc gcctggcaag
     2221 agcttctcct tcgactggaa ggccaattat cccggcgtct tcatgtacca ctgtggcgcg
     2281 ccaaccgtga tttatcacat ggccaatggc atgtacgggg cgatcatcgt tgatccggcg
     2341 accggttggg taccggcaaa ggaattcgtg ctggtgcaaa gtgaattcta cacgactcaa
     2401 ctcccggatg gctcgtactt tgtcgacccg aataagttga gcgccggcaa cccggactac
     2461 gtggtcttca atggctatgc caaccagtac aaaacgtcgc cgcttgtggc cagggcggga
     2521 gagcgtatcc gcatcttcat cgtgaacgca ggcccgacgc tgttctcggc cttccacgta
     2581 atcggagcca tcttcagcga tacctacgcc gacggcaacc cggccaacca catggtcgga
     2641 aaccagaccg tcacggtccc acccggcggc gccatgatgg tagagctcac catcccacag
     2701 gctggattgt acccattcgt aacccactca ttcgctgatg tcggaaaagg cgccatcggc
     2761 atcctcaaaa ttacaccgtg atcacgccat cacaaagcgc gatccagagg ggatgtcctg
     2821 gtacaaagga catccccaca cgacatcagt agcagcagag aacccgacca tgatggtaca
     2881 atgatgggat ggagcggcga gaaatgccca caacagcata tgagaaaatc aggcctctgc
     2941 caatgccgga aggggtacac aaacagcagc cgttctcgcg cgtagcccca atcctgaaga
     3001 cggcgatgct catgggtgtt ggcggcggct tcctgctggc aacgatcctg accgtcacat
     3061 acaccctctc cctaccgctc ggaacgtggt gggaggctct tgcgcaggcg catgggcatc
     3121 tccagttgta cggatgggcg ggactgttcg tgctgggcgt cgccttgcac tttctcccgc
     3181 gattacgagg cgcgcccctg gtcggcgcgc gcctcgttcc gtggctgctg ggcatcctca
     3241 tcgcaagcct ggtcttgcgc gccatcagcc agccgttgct gtcggtttct ccctcggctg
     3301 cctggcaaat aggactactg gtgagcagcg gcgttctcga agtggtcgcc tacgctgtca
     3361 tcctcgcgct gctggtactg accgcgctct ccggcccccg tccaacgaca cgcccggcct
     3421 attggagcgt cctcccgttc ttagcgggtg cgttctgctc gctgggcatc gccagcatcg
     3481 tcaacctgct gaatgtgatc caggccgcgc aggaaagcgg actggcaccg tacacgggcg
     3541 acacgctgaa tgtgacgctg ggactctttg gcttcctcgt cccggtggcg ctggcgatgt
     3601 cggcccgctc cctgccgatg tacgcggggc tggatggctt cccgcgccgc gtgctgtggc
     3661 cgctggctgg cgcgtacttc gctggggtca tactcctgtg tctcggttca ggtggaccgc
     3721 tggcagcgcc ctgggtcagc atcatcgatg gcctggggat ggcgctgctg ggaggggtcg
     3781 tgctgctgtt cgtcgggata ttcctgagcc tgatgcgcag gcgaggccga ctgcccgagc
     3841 gcgtggcgaa gctcgcgcca tcacccgaaa ccctggcaca gacctatcaa aagcaggtga
     3901 agcaggagca aacgcattac gggccattcg tggggctggt ggccagcgcc tatctctggg
     3961 ccatgctcgg cgcgctgctg ctgctcatcg atggaggcag tgtgctggca accggcagcg
     4021 aactggtcgc ttttgatgct atccgccatg cattcgccat cggctttctc gccctgctga
     4081 tctgtgggat tgccccgcgc atgctgccaa gcttctccgg cggcaggatc gcttctccga
     4141 agctcgtgag cgcaacgctc tggcttggca acgccgctgc cgtgttgcgt gttggcgctc
     4201 ttctcttcgc tccgctcttt gccggtaccc agggatttac cttcgatacc atcctctttg
     4261 gcctttcggg tccattcggt ttagcgatgg cgatctgcct ggcgatcaac ctctggccag
     4321 cattgtgagc ttcgcgaagg agccggtcca gcgtgagtcg agggtcgcaa aaaatttgga
     4381 agctatcgct tacccagctt gaccacgatg gcgctgatat tgtcttcacc tccacccgcc
     4441 aacgctgcct gcataagtgc ctgggccgtg tttgaagcat ccgtcccggg cgaggtcagg
     4501 atcgcggcga tctgctgatc gcgcaccatt tcccacaacc catcgg
//
LOCUS       DASZAK010001108         1528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_384754, whole genome shotgun sequence.
ACCESSION   DASZAK010001108 DASZAK010000000
VERSION     DASZAK010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384754"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VGT44_19630"
     CDS             <1..1312
                     /locus_tag="VGT44_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEV2583081.1"
                     /translation="GYDTDHQLAAGEFGKCGVAISSLADMEILFDGLPLDKITTSMTI
                     NSPASVIWAMYIVNAEKRGFPMAKLGGTLQNDILKEYSAQKEFLFPPEPSMRLVTDTI
                     EFGTRHMPRWNTISISGYHIREAGATAVQELAFTIADGLAYVDAALHRGLKIDEFAPR
                     LSFFFDVHNDFFEEIAKFRAGRRLWARLMRERYGAKDPRSWLMRCHAQSAGVSLTAQQ
                     PDNNVIRTAIQALAAVLGGTNSLHTNSLDEALALPTERAVLIALRTQQVIASESGVVN
                     TVDPLGGSYFVESLTDETERAAMDYLNRIDALGGVLACIQNGFFQREIAESAYRYQQE
                     IDQHRRTIVGVNDYIIEEDIKVPTLYIDREGERAHLERLNRVRRERDNAAVKRALENL
                     HRVAEGTENTMPAIIEAVKAYATLGEIMDVFRTVFGEYMEPAVF"
     gene            1325..>1528
                     /locus_tag="VGT44_19635"
     CDS             1325..>1528
                     /locus_tag="VGT44_19635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583082.1"
                     /translation="MPDTHTQGLIKKQILTEAEVTAIEQLIYACNNYEGLHMRLSLDA
                     LRKRTGNETNDFLYYEDGNLVGYL"
BASE COUNT          369 a          465 c          379 g          315 t
ORIGIN      
        1 cggctatgac accgaccatc aacttgcggc gggcgagttt ggcaaatgcg gcgtcgccat
       61 ctcttcgctt gccgacatgg aaatcctttt cgatggtctc cctctcgaca aaattaccac
      121 ttccatgacc atcaatagcc cggcttccgt catctgggcc atgtatatcg tcaacgccga
      181 gaagcgcgga ttccccatgg caaaattggg tggtacgcta cagaacgata tcctcaagga
      241 atacagcgcc caaaaagaat tcctgttccc gccagagcca tctatgcgcc tggtgaccga
      301 caccatcgaa tttggaacgc gtcacatgcc gcgctggaat actatcagca tcagcggcta
      361 tcacattcgc gaggcaggcg caaccgccgt ccaggaattg gctttcacca ttgccgatgg
      421 ccttgcctat gtcgacgctg ctctgcatcg cggcttgaag atcgatgaat tcgcgcctcg
      481 tctctctttc ttcttcgacg tgcataacga cttcttcgaa gaaattgcca agttccgtgc
      541 cggtcggcgc ctgtgggcca gacttatgcg cgagcgctac ggagccaagg atccacgttc
      601 atggctcatg cgctgccacg cgcagagtgc tggtgtctcc ctgacagccc agcagcccga
      661 caataatgtc attcgtaccg ccattcaggc cctggccgct gtactcggcg gcacaaactc
      721 cctgcacacc aattcgcttg acgaagcgct ggctctacct accgaaaggg ctgtactgat
      781 cgctttgcgc acgcagcaag tcattgccag tgagagtggt gtcgtcaaca cagttgaccc
      841 cctgggtggc tcctactttg tcgaaagtct gaccgacgag acagaacgag cggcgatgga
      901 ttacctgaat cgtatcgatg ccttaggtgg cgttctggcc tgcatccaga atggcttctt
      961 ccagcgtgaa attgcagagt cagcctatcg ttaccagcag gagatcgacc agcacaggcg
     1021 caccatcgtt ggcgtgaacg actatatcat cgaggaggac atcaaagttc ccacgctgta
     1081 catcgaccgc gaaggcgaac gtgcgcacct cgaacgcctc aaccgcgtcc gccgcgagcg
     1141 agacaacgcc gccgtcaaac gcgccttgga gaacctccat cgtgttgccg agggaacaga
     1201 aaacaccatg cccgccatca tcgaagccgt caaagcctat gccacgcttg gcgaaatcat
     1261 ggacgtcttc cgcaccgtct tcggtgaata tatggaaccc gccgtgtttt aagcaggagt
     1321 attaatgcct gacactcaca cacaaggcct tatcaaaaag caaatcctca cagaggcaga
     1381 agtaacagcc atagaacaac tcatatatgc ttgcaacaac tacgaagggc tgcatatgcg
     1441 actgagcctg gacgccttgc gcaaacgtac cggcaacgaa acaaacgact tcctctacta
     1501 cgaagatgga aacctcgtag gctacctc
//
LOCUS       DASZAK010001109         8061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_100834, whole genome shotgun sequence.
ACCESSION   DASZAK010001109 DASZAK010000000
VERSION     DASZAK010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100834"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /gene="hisI"
                     /locus_tag="VGT44_19640"
     CDS             complement(<1..587)
                     /gene="hisI"
                     /locus_tag="VGT44_19640"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: protein motif:HMM:NF000768.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEV2583083.1"
                     /translation="MLNFDRQGLIPVAIQDDTTNEVLMLAFMNEEAYRLTRETGYTHF
                     YSRSRQAIWRKGEQSGHVQEVRGIFVNCEENSLLMRVKQHGGAACHTGYRSCYYRRIQ
                     PDGSYEIIAERIFDPEAVYGTAIQEEVEVALESSLRKLYDVYQFLRDRDLSEQSNTSR
                     LLHESLEPASGTTGYLLSRLADELEELMEVQSGEHV"
     gene            complement(574..1347)
                     /gene="hisF"
                     /locus_tag="VGT44_19645"
     CDS             complement(574..1347)
                     /gene="hisF"
                     /locus_tag="VGT44_19645"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEV2583084.1"
                     /translation="MLTRRIIPCLDVTEGRVVKGVNFLNLRDAGDPVELAAFYNAEGA
                     DELVFLDITASYEKRATMLDVVRRTAEQVFIPVTVGGGIRSVEDIRATLGAGADKTSL
                     NTAAVQNPALLREGAEQFGAQCIILAIDARANAAMPSGYEVFIHGGRTPTGLDVVEWA
                     RRGVSLGAGEILLTSMDRDGTQQGYDLGLTALVSAAVTVPVIASGGVGTLEHLYEGFA
                     KGGADAALAASIFHFGQFRISDAKRYIAAKGLPVRYVEL"
     gene            complement(1418..2149)
                     /gene="hisA"
                     /locus_tag="VGT44_19650"
     CDS             complement(1418..2149)
                     /gene="hisA"
                     /locus_tag="VGT44_19650"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEV2583085.1"
                     /translation="MIILPAIDIKNGQCVRLYQGDFARATVYDADPAQVARRFEEAGA
                     LWLHIVDLDGALSGQPVNVGVIDQIRAATSLRIELGGGLRRLNHIESMLSIGIDRVIL
                     GTTALMNRDFLRDALARWGERIAVGLDAREGRVAIAGWQKISRVQATILAKELVAEEG
                     VSRFIYTDIARDGAMQGPNLPALREMQQTIADTGASLIASGGVHSLDDLRALAALGVE
                     GAIVGKALYTGDVKLEEALRMFNSP"
     gene            complement(2146..2814)
                     /gene="hisH"
                     /locus_tag="VGT44_19655"
     CDS             complement(2146..2814)
                     /gene="hisH"
                     /locus_tag="VGT44_19655"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEV2583086.1"
                     /translation="MIAIVDYGAGNIRSIEKALAYLGARVEVTDDAAVVARASAVVLP
                     GVGSGGAAMARMKQRGLDDAIRQATEQYRPFLGICLGMQLLADHHAEGEVDGLGLFRG
                     EVRRIGWGAYVERSQHSDAGGGPHGPKIPHMGWNQVMPLRDGLPIFAGIPADAYFYFA
                     HSYYVEPQDQNGVAAVTDYGSPFCSVIVSEQCWGTQFHPEKSGPVGLRMLRNFIEWVE
                     DREP"
     gene            complement(2811..5420)
                     /locus_tag="VGT44_19660"
     CDS             complement(2811..5420)
                     /locus_tag="VGT44_19660"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEV2583087.1"
                     /translation="MSNLIVFDLDGVITSEAAYWDAAGLALGEVMYSPYYWNLAGGNP
                     GAAHVALQSTAPYHAPTKAAASRSLSRAIFPEPEIQALKARAINSNWDTTYAAVCLRL
                     VDLLARLPAASLFPLRPWDTRWIAAFRETLASDSQDVLQKARPFSLLAALEAPLLSGY
                     TGMELINRFDAYASERLGRVIQGVFSRYSPFWEFCRDIFQQWFLGDELYARTYGHAPA
                     QPGKPGCIHFEQPLLPLEQTRATLETLRRQGYILGIATGRDRQEAIYPLKQYGLLAYF
                     DEQRISTYDDVERAEAILREKGDQTLLTKPHPFPFLVAAYSVSKTGEALSLPGTRATQ
                     AFPPRPESTPAPTVGTAAGSPYSPFVVVGDSTSDVLGGRAAGAITVAVLTGARTVEAR
                     ERLAQSGPDFTIGNITELPGLLADIDSLATIQRLQFLEREKAERLLRRWFERHMRLAV
                     EDVTLTPKAVSLNSFNGFYRSDGEEFFFKTHVEEQGTLEEYYHAERLSQAGYNIVQPL
                     RTLHEGGRQMVIYPVVRWPVVFDLMRAVETGDAEKSMGVTAEALTAAERAECARLLDI
                     YRSTLQFSTAEEHAQAPVHQLFWHRLAGERLKTFYEGKQIALPAGNGDLPFDALLRCR
                     WRINGALQQHTLGELIEQGKSVLRPERAMMTVVGHGDAHFGNVFLEELQGYLYFDPAF
                     AGRHAPLLDVVKPLFHNIFATWMYFPREVARDVCVTVSLKQGSDGHPERGEGLQDGGI
                     CHPERSEGSNAGAREILRCAQDDNIEEGGNAKEGDIIEVKHDFTLTAVRQAILRAKVE
                     HLLRPLVAELAARVALPDDWREIVQLALMCCPLLTINLADGERFPPEISWLGLSLAVQ
                     MGNSGISSWGFEL"
     gene            complement(5417..6508)
                     /gene="hisG"
                     /locus_tag="VGT44_19665"
     CDS             complement(5417..6508)
                     /gene="hisG"
                     /locus_tag="VGT44_19665"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEV2583088.1"
                     /translation="MSAEAVIRLAVPGKGTLEASTLTFLAECGMRVKRSNPRQYIAQI
                     ESIPDLEVVFLRAADIPLLVQDGDATLGITGYDILAEHRGTGDGTDDEGENFDTEHEE
                     LIVLERNLGFGRCRLVVAVPEDWIDVSTCADLWHLAGYYQAHKGRSLRVATKYPILTE
                     QFLRRHGITRCKIVSPHGALEAAPLTKTVDFIVDLTETGTTLRENHLKLLDDGEVLQS
                     QACLIANASLLRQNERALRLTSMMLELMEARVQARNRSLLTAYMQAESAEEKHRACAR
                     LYQRLKALVSSREFQVSSSEQVGEAKSQWYTISCVVNTGQSAAELLEVVAIFRGAGAV
                     RIDTVPLNYRFAEESMNVRTLRERLKRTH"
     gene            complement(6505..8034)
                     /locus_tag="VGT44_19670"
     CDS             complement(6505..8034)
                     /locus_tag="VGT44_19670"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HisS family protein"
                     /protein_id="HEV2583089.1"
                     /translation="MKKRVERLRGMHDLLPEEYLRQRKVIERMMNDLALAGYSPVDAP
                     VLEQSDLFHNSFGQELWQNLYTFRLHHRDLCMRPEYTASICRMYLDHFQQHPLPLRFQ
                     YAGPIFRYEAPGRNRYRQHTQLGVELFGGHVASADAEILQLACDQLNQLGITRCQVEL
                     GHIGIASGFINRLRLDDHAARLLLSLMEQVSRSEQGEQAAQSRLETLYPASARGNEMI
                     VDAAGATGTADAAEPGAGGETRLIASLMSSIRMPFNDETRQEIIDRYLWKMGRGEQGR
                     QIQRALDFLRALHATAGSPPGVFAALRDLLKSYEIETEELRNVEQLVGVIQQCGVPGE
                     NIIVNLSLGRGVNYYTGLVFEIHAQDDEGYDTQLCGGGRYDRLIRMVGGSRDVNACGF
                     AFGVDRLLALLPKNELPLVNAIEALVIPVSAQDMAYALRVARAARAGGMTTELDVSGH
                     GVGAGLKLAARRQARLALIVGEDERRAGLVTVRDLEARDEQRLPVEQLATVVVMKEQR
                     L"
BASE COUNT         1568 a         2719 c         2243 g         1531 t
ORIGIN      
        1 acatgctcgc cgctctgcac ctccattaac tcctccaact cgtcagcaag ccgcgaaagc
       61 aggtagccgg tcgtgccgct cgccggttcc aggctctcgt gcagcagccg cgacgtgttg
      121 gactgctcgc tcaaatcacg atctcgcaag aactggtaaa cgtcgtacag cttgcgcagg
      181 cttgattcca gcgcaacttc tacttcctct tgtatggccg ttccatacac tgcctccggg
      241 tcgaagatgc gttccgcgat gatttcatac gagccatctg gctggatgcg tcgatagtaa
      301 caactgcgat agccggtatg gcaggccgcg ccgccgtgct gcttgacacg catcagcagg
      361 ctgttctcct cgcaattgac gaagatgccg cgcacctcct gaacatggcc cgactgctcg
      421 cctttgcgcc agatcgcctg gcgcgagcga ctataaaagt gcgtatagcc cgtctcgcgc
      481 gtcaggcggt aggcttcctc gttcataaaa gccagcatca gcacttcgtt ggtggtatca
      541 tcctggatgg cgacagggat caacccctgg cgatcaaagt tcaacatagc gcaccggtaa
      601 tccttttgcc gcaatatagc gcttcgcgtc actgatacga aactgtccaa aatgaaagat
      661 cgaggccgcc agcgccgcat ccgcgccgcc cttcgcaaag ccctcgtaga gatgctccag
      721 cgtaccgacg ccgcccgatg cgatcaccgg caccgtcaca gccgccgaga caagcgcggt
      781 caggcccagg tcatagccct gctgcgtccc gtcgcggtcc atgctggtca gcaggatttc
      841 gcccgctcct agcgacacgc cgcgccgcgc ccattcgacg acatccagcc ccgtcggcgt
      901 gcgacctcca tgaatgaaga cctcgtagcc gctaggcatg gcagcgttgg cgcgcgcgtc
      961 gatggccaga atgatgcact gcgctccaaa ctgctccgcg ccctccctca gcagcgccgg
     1021 gttctgcacc gctgccgtgt tcagcgaggt cttatcagcc cccgctccca gcgtcgcgcg
     1081 gatatcctcc acgctgcgaa tgccgccgcc aaccgtgacc ggaataaaga cctgctctgc
     1141 cgtgcgccgc accacgtcaa gcatcgtggc gcgtttctca tacgaggcag tgatatccag
     1201 aaacaccagc tcgtccgcgc cctccgcatt ataaaacgcc gccagttcca ccgggtcccc
     1261 cgcgtcgcgc aaattgagga aattgacccc cttcacaacg cgcccctcgg tcacatccag
     1321 gcacggtatg attcgcctgg taagcatgat tgcacctcac attgtcgatg ccggttatac
     1381 gcattggcaa cttaacacat tcatcgtcgc cacgtcccta cggactgttg aacattctca
     1441 gcgcctcttc cagctttaca tctcctgtat acaatgcttt gcctacgata gctccctcga
     1501 cgcccaacgc agccagcgcg cgcaggtcgt cgagcgaatg cacaccgccg gaggcgatca
     1561 gcgacgcgcc tgtatccgcg atggtctgct gcatctcgcg cagggcaggc agattggggc
     1621 cttgcattgc gccgtcgcgg gcgatgtcgg tatagatgaa gcgcgagacg ccttcttcgg
     1681 cgacaagttc ttttgcgagg atggtggcct gtacccgcga gatcttttgc cagcctgcga
     1741 tggccacgcg accttcgcgc gcgtcgaggc cgacggcgat gcgctcgccc cagcgcgcca
     1801 gggcatctcg caggaagtca cggttcatca gcgccgtcgt gccgagaatg acgcgatcaa
     1861 tgccaatgct cagcatggac tctatatgat tgagcctgcg caggccccca ccgagttcga
     1921 tacgcagcga tgttgcggct cgtatctggt cgatcactcc aacgttgacg ggctggccgg
     1981 agagcgcgcc atccaggtct actatgtgca gccagagcgc ccctgcctcc tcgaaacgcc
     2041 ttgccacctg cgccgggtcg gcatcataca cggtggcccg ggcgaaatcg ccctggtaga
     2101 ggcgcacgca ctggccgttt ttgatgtcga tggcgggcag tataatcacg gttctcgatc
     2161 ctccacccac tcgataaagt tcctgagcat tctcaatccc accgggccgc ttttctccgg
     2221 gtgaaactgc gtcccccagc actgttcgct cacgatcacg ctgcaaaatg gcgatccata
     2281 atccgtgacg gcagccactc cgttctgatc ctgcggctcg acatagtagg aatgggcaaa
     2341 gtagaaatag gcgtccgccg ggataccggc gaagataggc aggccatcgc gcagcggcat
     2401 gacctggttc cagcccatat gcgggatctt cggcccatgc ggtcccccac ccgcatcgct
     2461 atgttggctt cgctcaacat acgctcccca ccctattcgc ctcacctcac cccggaagag
     2521 gcccaggcca tcgacttcgc cctcggcatg atggtcggcc agcagctgca tccccaggca
     2581 gatgcccagg aatgggcggt actgctccgt tgcctgccga atggcatcat ccagcccgcg
     2641 ctgcttcatg cgcgccatcg ccgcgccgcc agaaccgacg cccggcagca ccaccgcgct
     2701 cgccctggcg accaccgccg cgtcgtccgt cacctccacg cgcgcaccca ggtacgccaa
     2761 cgccttctcg atgctgcgga tattacccgc gccataatca acaatcgcaa tcatagctcg
     2821 aacccccagg aacttatgcc gctatttccc atctgcacag ccagcgatag acccagccag
     2881 cttatctcag gcggaaagcg ctcgccatcc gccaggttga tggtcaacaa cgggcagcac
     2941 atcagcgcca gctgcacgat ctcacgccaa tcgtcgggca aagcgacacg cgccgccagt
     3001 tccgcaacga gcggcctcag cagatgctcc accttcgccc gcaagatggc ctgccgcacc
     3061 gccgtcaagg taaaatcatg tttaacctca ataatgtcac cttccttggc attgccaccc
     3121 tcctcaatat tgtcatcctg agcgcagcga aggatctctc tcgcgcccgc attagatcct
     3181 tcgctgcgct caggatgaca aatgccgccg tcctgaagtc cctcgccgcg ctcaggatga
     3241 ccgtcgctgc cctgctttag cgaaactgtc acacacacat cgcgcgccac ctctcgtggg
     3301 aaatacatcc acgtcgcgaa gatgttgtga aagagcggct tgactacgtc cagcagcggc
     3361 gcatgacgcc ccgcgaaggc cgggtcaaag tagaggtagc cctgcagctc ctccaggaag
     3421 acgttgccga aatgcgcgtc gccatgccca acgacggtca tcatggcgcg ctccgggcgc
     3481 agcacactct taccctgttc gatcaattct ccaagtgtat gctgctgcaa cgcgccattg
     3541 atgcgccagc gacagcgcag caacgcatca aacggcagat cgccatttcc agccggaagc
     3601 gcgatctgct tcccctcgta aaacgtcttg agccgctcgc cagcaaggcg atgccagaaa
     3661 agctggtgaa ctggcgcctg tgcgtgttcc tccgccgtac tgaattgcag cgtggagcgg
     3721 taaatatcga gcagccgcgc gcattccgcc cgctcagcag ccgtgagggc ctcagccgtc
     3781 acccccatgc tcttctcagc gtcgcccgtc tcgacagcgc gcatcaaatc aaacacaacc
     3841 ggccagcgca ccaccgggta aatcaccatc tgccgcccgc cctcgtgcag tgtacgtaac
     3901 ggctggacaa tgttataccc tgcctgagac agccgttcgg catgataata ctcttcgagc
     3961 gtcccctgct cttcaacatg cgtcttaaag aaaaactcct cgccatcgct gcgatagaag
     4021 ccattgaagg agttaagcga aacggcctta ggcgtcaaag tcacatcctc taccgccagg
     4081 cgcatatgcc gctcaaacca gcgccgcagc agccgttccg ccttctcacg ctccaggaat
     4141 tgcagccgct ggatggtggc aaggctatcg atatccgcaa gcaatcccgg cagctccgta
     4201 atattcccaa tcgtgaagtc agggccgctc tgcgccaatc gttcgcgcgc ctccaccgtg
     4261 cgcgcccccg tcagcaccgc caccgtaatc gcccccgccg cccgcccccc caaaacatcg
     4321 ctggtagaat cccccacaac cacgaacggg gaatacggcg atcccgctgc cgtgccgacc
     4381 gtaggggcgg gggtggactc ggggcggggt gggaacgctt gcgtcgcccg cgtacccggt
     4441 agcgatagcg cttcccccgt cttggacaca ctatacgccg ccacaagaaa cggaaacggg
     4501 tgcggcttgg tcagcaaggt ctgatcgcct ttttcgcgca aaatggcctc ggcgcgttcg
     4561 acatcgtcgt aggttgatat gcgctgttca tcaaaatagg ccagcaggcc gtattgtttg
     4621 agcggataga tggcttcctg gcgatcgcga ccggtggcga tgcccagaat gtatccctgc
     4681 cgtcgcagcg tctccagcgt cgcgcgcgtc tgttccaggg gcagcagggg ctgctcgaag
     4741 tggatgcagc cgggcttgcc gggctgcgca ggcgcgtggc cgtaggtgcg ggcatacaac
     4801 tcgtcgccca ggaaccactg ctgaaagatg tcgcggcaaa attcccagaa gggactatag
     4861 cgcgagaaaa cgccttgaat aacacgcccc agtctttcag aggcatacgc atcgaagcgg
     4921 ttgatgagtt ccatgccggt atagccactc agaagcggcg cttctagcgc ggcaagcagg
     4981 ctgaacgggc gcgccttctg tagaacgtcc tgcgaatcgc tcgccagtgt ttcgcgaaac
     5041 gcggctatcc agcgcgtgtc ccaggggcgc aagggaaaca gcgaggcagc gggcagccgc
     5101 gcgagcagat cgaccaggcg cagacacacc gccgcgtagg tggtatccca gttggaattg
     5161 atggcgcgcg ccttgagagc ctgtatctcc ggctccggga agatggcgcg actcaggctg
     5221 cggctcgcgg cggccttcgt gggcgcgtgg tagggcgcgg tggattgcag cgccacgtgc
     5281 gcggccccag ggttgccacc ggccaggttc cagtaatacg ggctgtacat cacctcgccc
     5341 agcgccaggc ccgccgcgtc ccagtacgcc gcctcgctag tgatgacgcc atccaggtca
     5401 aagacgatca ggttgctcaa tgcgttctct tcaatcgctc gcgcagtgtg cgcacgttca
     5461 tcgactcttc cgcgaaacgg tagttcagcg gtacggtatc aatacgcacg gccccggcgc
     5521 cgcgaaagat cgccaccact tccaacaatt cagcggcgga ttgcccggtg ttgacgacac
     5581 atgaaatagt ataccactgc gacttcgcct caccaacctg ctcggaactc gatacctgga
     5641 actcgcggct cgacacgagc gctttgagtc gctggtagag ccgggcgcag gcgcgatgct
     5701 tctcttcagc gctctcagcc tgcatatagg ctgtcagcag cgaacggttg cgcgcctgca
     5761 cgcgcgcctc catcaactcc agcatcatcg aggtcaggcg caaggcgcgt tcgttctggc
     5821 gcagcaggct cgcgttggcg atcaaacacg cctgtgattg cagcacttcg ccgtcgtcga
     5881 gcagcttcaa atgattttcg cgcagcgtcg tacccgtctc ggtcaagtct acgataaagt
     5941 cgaccgtctt ggtgagcggc gcggcctcta acgcgccatg cggggagacg atcttacagc
     6001 gcgtgatgcc atgcctgcgc aggaattgct ccgtcaggat aggatacttt gtcgctacgc
     6061 gcaggctgcg ccccttgtgc gcctggtaat aaccggccag gtgccagagg tcggcgcagg
     6121 tactcacgtc gatccagtct tctggcacag ctacgaccag gcgacagcgg ccaaagccga
     6181 ggttacgttc cagcacgatt aactcttcgt gttctgtatc gaagttttcg ccttcatcgt
     6241 cagtgccatc gcccgttccg cgatgttcgg ccaggatatc atagccggta atgcccagcg
     6301 tcgcatcccc atcctgcacc agcaggggaa tatcggcggc gcgcaggaag actacctcaa
     6361 ggtcggggat gctctcgatt tgcgctatat actggcgcgg gttgctgcgt ttgacccgca
     6421 ttccgcactc ggcgagaaag gttagcgttg atgcttccag cgtccctttg cccggaacgg
     6481 ctaaacgaat aacggcttcc gcgctcatag tcgctgctcc ttcattacaa cgactgtggc
     6541 cagttgctcg accggcagac gctgctcgtc gcgcgcttcg aggtcgcgca cggtgacgag
     6601 tccggcgcgc cgttcgtctt cgcccacgat cagcgccagc cgcgcctgcc tcctggccgc
     6661 cagcttcagg cccgcaccga cgccatgccc gctcacatcg agttcggtcg tcatgccgcc
     6721 cgcgcgagcc gcccgcgcca cacgcagggc ataggccata tcctgcgcgc tgacggggat
     6781 caccagcgcc tcaatcgcgt tgacgagcgg cagttcattt ttgggcaaaa gcgccagcag
     6841 gcgatcaacg ccgaaggcaa agccgcaggc attcacatcg cgcgaaccgc ctaccatgcg
     6901 gatcagccgg tcgtagcgcc cgccgccgca gagctgcgta tcgtacccct cgtcgtcctg
     6961 tgcatggatc tcgaagacca gcccggtata ataattcacc ccgcgcccca acgaaagatt
     7021 gacgatgata ttttcgcccg gcacgccaca ctgctggatg actcccacca gttgctctac
     7081 gtttctcaac tcctccgtct cgatctcgta gcttttgagc aaatcgcgca gcgcggcgaa
     7141 gacacccggc gggctgcctg ccgtggcatg cagcgcgcgc aggaagtcga gggcgcgctg
     7201 gatctgccgc ccctgctcgc cgcgccccat cttccacagg tagcggtcga taatctcctg
     7261 gcgcgtttca tcattgaacg gcatcctgat gctgctcatc aacgacgcga tcaggcgcgt
     7321 ctctccgccc gctccaggct ccgccgcgtc cgctgtgcct gttgcgcccg ctgcatcgac
     7381 aatcatctca ttgccacgcg cgctggccgg gtacaacgtt tccaggcgcg actgcgccgc
     7441 ctgctcgcct tgctcggaac ggctgacctg ctccatcagg ctgagcagca ggcgcgcggc
     7501 gtgatcgtcc aggcgcaggc ggttgatgaa gccgctggcg atgccgatat gccccagttc
     7561 tacctggcag cgtgtgatgc ccagttgatt gagctgatcg caggccagtt gcaatatttc
     7621 ggcgtcagcc gaggccacat gaccgccgaa tagctccaca ccaagctgcg tatgctggcg
     7681 atagcgattg cgcccaggcg cttcgtagcg aaaaatcggc ccggcgtact ggaaacgcag
     7741 cggcagggga tgctgctgga aatgatccag gtacatgcgg cagatcgatg cggtatactc
     7801 cgggcgcata catagatcgc ggtgatgcag ccgaaaagtg tacaggtttt gccacagctc
     7861 ctgaccgaag ctgttatgaa agaggtcgct ctgctccagc accggcgcat cgacggggct
     7921 ataccccgcc agcgccaggt cattcatcat acgctcgatc accttgcgct gccgcaggta
     7981 ttcttccggc agcagatcat gcatcccacg cagccgctca acgcgctttt tcatactatg
     8041 cttccttcag gcatctgcgc g
//
LOCUS       DASZAK010001110         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_28449, whole genome shotgun sequence.
ACCESSION   DASZAK010001110 DASZAK010000000
VERSION     DASZAK010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28449"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..858)
                     /locus_tag="VGT44_19675"
     CDS             complement(79..858)
                     /locus_tag="VGT44_19675"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEV2583090.1"
                     /translation="MPNTILSQLINELHSGRLRVVDLTTPLGPDTPVIDLPPMFAPSP
                     GLSMSEISHYDDRGPAWYWNTLTLGEHTGTHFDAPVHWVTGKDLPNNATDTILPEKFV
                     GPACVIDVTADVEQDADFLLTVERVEQWESQYGRLPAGAWLLVRSGWSRHKGRAEFLG
                     LRDGGSHSPGFHPTCSAFLAKERAILGVGVETVGTDAGAAGGFDPPFPSHTFMHGAGK
                     FGITSLVNLDQLPATGAIVIAAPLKIVNGSGSPLRVIAITP"
     gene            complement(883..1464)
                     /locus_tag="VGT44_19680"
     CDS             complement(883..1464)
                     /locus_tag="VGT44_19680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEV2583091.1"
                     /translation="MLRIEALTAAYPELENRIVVTIMGAVAAELYALGHRHNFFYLEH
                     AMGLASSMGLGIALAMPQHKVIVLDGDGSLLMNLGTLTTMARYSPGNLLHIVFDNESL
                     LSVGGFPTATSTGADLAGIARASGIPSVMEANTPASFADSVRGALASDTLTTIISKVD
                     AIGPKSFHMDVGLLENRFEFKRALAAMKGQTKE"
     gene            complement(1468..1701)
                     /locus_tag="VGT44_19685"
     CDS             complement(1468..1701)
                     /locus_tag="VGT44_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583092.1"
                     /translation="MEKRNLICYRGHFGEKDPWQTQGGLATEPLLRALNIPYCMVSSP
                     GDVARRVKDAQTMANASLHPVAVLLTRELMWEE"
     gene            1881..2393
                     /locus_tag="VGT44_19690"
     CDS             1881..2393
                     /locus_tag="VGT44_19690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfopyruvate decarboxylase"
                     /protein_id="HEV2583093.1"
                     /translation="MPVSVNNSRLIYEALKACDVKIISALPETWLVHLIRLAEEDPDM
                     TLVRLAKEEEGVGISAGAHLAGVNSAMLMQNHGFLASINGIVSLALLYKIPLTLFISY
                     RGHFGEKDPWQTQGGLVTEPVLRALNIPIWHLNNVVDLQRRINDAYTLAHASLHPVAV
                     LLHREVMWEE"
BASE COUNT          550 a          700 c          709 g          570 t
ORIGIN      
        1 acaaattcat attgatcttg gtcatgtaac ttcatcattg cgtctctacg gcgagggcaa
       61 ttcggttatg catgcccgct acggtgtgat ggcaatcaca cgcaaagggc tgcctgagcc
      121 gttaacgatc ttgagcggcg cagcgatcac gattgccccg gtggcgggca gttgatccag
      181 gttcaccagg ctggtgatgc cgaatttgcc cgcgccatgc ataaaggtat ggctgggaaa
      241 gggtgggtca aagccgcctg ctgcgcccgc gtctgtccct actgtctcaa cgcctacacc
      301 cagaattgcg cgttcctttg ccaaaaacgc cgagcaagtg ggatgaaaac cgggcgaatg
      361 cgagccgccg tcgcgtaaac ccaggaattc cgcgcgtccc ttatgcctgc tccaaccgct
      421 gcgtaccagc agccacgcgc ccgcaggcag tcgcccatac tggctctccc attgctctac
      481 ccgctcaacg gtcagcagaa aatccgcgtc ttgctcgaca tcggcggtga catcgataac
      541 acaggccggg ccgacgaact tttctggcag gatcgtatcg gtcgcgttgt tgggcaggtc
      601 tttgcctgtc acccagtgta cgggcgcgtc gaaatgcgtc ccggtatgct cgcccaacgt
      661 cagcgtgttc cagtaccacg ccggcccacg atcatcatag tgcgaaatct cgctcatact
      721 caaccctggc gacggcgcga acatcggcgg aagatcaatg acaggcgtat ccggccccag
      781 cggcgtagtg agatcaacca ccctcaagcg accggaatgc agctcattga tgagctgcga
      841 gagaattgtg tttggcatga tcggttcctt tcctacgtag ctttattctt tcgtttgccc
      901 cttcatcgct gccagcgcgc gtttgaactc gaagcggttc tccagcagac ccacatccat
      961 atggaacgat ttgggaccga tagcatcaac cttcgagatg atggttgtga gcgtatcgct
     1021 cgccagcgcg ccgcgcacgc tgtccgcgaa actggcgggc gtgttggcct ccataaccga
     1081 ggggatgccc gatgccctgg cgatgccggc caggtctgcg ccggtcgagg tcgcggtggg
     1141 aaagccgccg accgagagca ggctttcgtt gtcgaacacg atgtgcagca ggttgcccgg
     1201 actgtagcgc gccatcgtcg tgagcgtccc caggttcatc agcagcgagc catcgccgtc
     1261 cagcacgatc actttgtgct gcggcatggc gagcgcgatg cccagtccca tcgacgaggc
     1321 caggcccatg gcgtgttcga ggtagaagaa gttgtggcga tggcccaggg catagagttc
     1381 ggcggccacc gcgcccatga tggtgacgac gatgcggttt tccagttcgg gatacgccgc
     1441 cgtcagcgct tcgatgcgta acatggccta ttcctcccac atcagttcgc gagttaagag
     1501 gactgctacc gggtgcagcg aggcattcgc catcgtttga gcgtctttga cgcgccgcgc
     1561 cacgtcacca ggcgaggaga ccatacagta gggaatgttg agggcgcgca gcagcggctc
     1621 ggtcgccaat ccgccctgcg tctgccaggg gtctttctcg ccgaagtggc cgcgatagca
     1681 gataaggtta cgcttttcca ttgtcaagat gtactcctgt ctcggttgtg ctggctgaac
     1741 taaaggacat tttagcacaa tgccctgtgt gaaagacagg gcaagaaagg attcaatcta
     1801 tcaagtgtta tcaagtttct aaataacatg ctatactaaa gactatcata ggttggcttc
     1861 tatattttgg ggaggctgct atgcccgttt cggtaaacaa ttccagattg atctacgagg
     1921 ctcttaaagc gtgcgacgtg aagattattt ctgcgctgcc tgaaacctgg cttgttcatt
     1981 taatccgttt ggctgaggaa gaccctgaca tgacgcttgt acgtcttgcg aaagaggaag
     2041 aaggcgttgg tatttcagcg ggggcgcatc tggcgggcgt caactctgcg atgctcatgc
     2101 aaaatcatgg ctttctggca tcaatcaatg gcatcgtctc actggcactg ctctacaaga
     2161 taccattgac gctatttata tcgtacagag ggcattttgg ggaaaaagac ccctggcaaa
     2221 cgcaaggggg cctggttaca gagccagttt tgcgtgcgct gaacatcccg atttggcatc
     2281 tcaataatgt cgttgatctt cagcgccgta ttaatgatgc gtatacgcta gctcatgcgt
     2341 ccttacatcc agttgccgtc ttactgcatc gtgaagtgat gtgggaagaa taaccaataa
     2401 tgaactcaca caagggatag cgtcatgaag cggcacatag acccatcaaa gcggcttgtc
     2461 caggaatttc atcctggaca gccacaattg tccatagaca agcttgtggt tcagtacatc
     2521 aggcaaagc
//
LOCUS       DASZAK010001111         5220 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_668804, whole genome shotgun sequence.
ACCESSION   DASZAK010001111 DASZAK010000000
VERSION     DASZAK010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5220
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668804"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VGT44_19695"
     CDS             <1..846
                     /locus_tag="VGT44_19695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2583094.1"
                     /translation="YRSVKVDGLSIFYREAGSKDTPTILLLHGLPSSSRMFEPLFTRI
                     SDRYHLVAPDYPGFGHSDWPDPKTFAYTFDHYAQIMNHFTEALGLSRYTLYMQDYGGP
                     VGFRMALADPDRIEALIVQDAVAHNEGLGANWQPRRAFWADRAANESTLRTNLLSLPT
                     TRTRHVGNDPNVERYDPDLWTDEFAFLTQPGQADIQSDLFYDYRTNVDAYPKWQAWMR
                     EKQPRLLVLWGKYDLSFELSEPEAYRRDVPNAQVHVLDAGHFALDTAADEIAALVRGF
                     VASSR"
     gene            complement(1497..2660)
                     /locus_tag="VGT44_19700"
     CDS             complement(1497..2660)
                     /locus_tag="VGT44_19700"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2583095.1"
                     /translation="MPGTFRIGKIAGIDIDIHVSWIIILVFLTVSLDTGWFPQLYPGW
                     STATYWLIAFLSSLLLFVSVLLHELAHSLVARRRGLPVKSITLFIFGGVSNIEREPQS
                     PGVEFQIAFVGPLTSLVIGAICFLLQVPLRGSNSPLEGILFYLAVTNILLGVFNLIPG
                     FPLDGGRVLRSIVWRLTGNLRQATRVASLTGQVIAYLFILLGIWIFFVGDILDGIWFG
                     FIGWFLLSAAQSANAQGMLTSVLSGVTVGEVMNPKPATVPADISLQQLVDAYFLPGGL
                     RYALVMQADHLVGLITLGDIRHIPREQWGQVPVSTAMIPLSRLHVVTPQQSLSDVLPL
                     MAGRDVNQLPVLENDALVGILSRDAIVHYLEVRRSLGVENAQSDAHNHLSRAA"
     gene            2774..3133
                     /locus_tag="VGT44_19705"
     CDS             2774..3133
                     /locus_tag="VGT44_19705"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEV2583096.1"
                     /translation="MAFFFPYLSLAELFSASRFPGAFRELLGLSTRLAESAYPDESQY
                     LAPCSMIDTEERSYALASLPPKLRSIVHLRCFRQLTFAEIGRTLNMPEPTVKTYFYRS
                     LPLTRRALAATATCHVH"
     gene            complement(3256..3927)
                     /locus_tag="VGT44_19710"
     CDS             complement(3256..3927)
                     /locus_tag="VGT44_19710"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEV2583097.1"
                     /translation="MFHLATSTLTHLLATYGYLAVLLFVTIESTGIPFPGETILLVAA
                     IAAGTTHQLSIAWVIVAAASGAILGDNLGFWVGREGGYRLLRRYGRYIHLEERRLKLG
                     QYLFLKHGGKVVFFGRFVAVLRAWAAFLAGTNRMRWPRFLLFNAAGGIVWATLYGLGG
                     YFLGDNIHRLVGPVGIVLLVLAVLLIIAGIFIVRRNEERLADEAERALPGPLAISLPR
                     GKRPG"
     gene            complement(3997..4323)
                     /locus_tag="VGT44_19715"
     CDS             complement(3997..4323)
                     /locus_tag="VGT44_19715"
                     /inference="COORDINATES: protein motif:HMM:NF023010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 domain-containing protein"
                     /protein_id="HEV2583098.1"
                     /translation="MKGEVLPGVGEWLLVRSDGVGELDTRITLQTDDGALIYETFRGY
                     LTRVAELLPRWMAGEQIPNEEYYFVITPHFETSASQYAWIQHEVTIGMGSLIPGGVSF
                     QVYAVR"
     gene            complement(4320..4445)
                     /locus_tag="VGT44_19720"
     CDS             complement(4320..4445)
                     /locus_tag="VGT44_19720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583099.1"
                     /translation="MIQHDKQTQPESAETTPTRFVHVREVRFAYRRFGNPVGTLA"
     gene            complement(4466..5170)
                     /locus_tag="VGT44_19725"
     CDS             complement(4466..5170)
                     /locus_tag="VGT44_19725"
                     /inference="COORDINATES: protein motif:HMM:NF037020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583100.1"
                     /translation="MRVEYPIAIDNDYAIWSAFNNHYWPALYLVDAQGRIRSHHFGEG
                     AYEQSEMMLQQVLAEAGIGGFAHELVSVDAQGAEAAADWGDLRSPENYVGDARTENFA
                     SPGGAVLDQPRVNTAPARFSLNQWALAGEWTVGEQAVALHTANGRIAYRFHARDLHLV
                     MGPAARGTSVRFRVFLDGQAPGAAPGSDVDEQGHGTATEQRLYQLIRQPGHIADRQFE
                     IEFLDAGVEAFAFTFG"
BASE COUNT         1110 a         1575 c         1488 g         1047 t
ORIGIN      
        1 tatcgctcgg tgaaggttga cggattgtca atcttctatc gggaagcagg atcgaaggat
       61 acccccacga ttctcttgct ccacgggctg ccctcttcgt cccgcatgtt cgagccgctc
      121 ttcacccgga tctccgatcg ctaccacctg gtcgcgccag attacccggg cttcggacac
      181 agcgactggc cggacccgaa aacattcgcg tacacgttcg atcactacgc ccagatcatg
      241 aatcacttca ccgaagcgct cgggctctcg cgctacacgc tgtacatgca ggattacggt
      301 ggccccgtag gttttcgcat ggccttggcc gatccggacc gtatcgaggc tctcatcgtc
      361 caggatgctg tggcgcacaa cgaaggcctg ggggccaatt ggcagccgcg gcgggccttt
      421 tgggccgatc gcgcggccaa cgaaagtacg cttcgcacca atctcctgtc gctaccgacg
      481 acgcggacgc gccatgtcgg caacgatcct aacgtggaac gctatgaccc ggatctgtgg
      541 accgatgaat ttgcctttct cacccagccc ggccaggccg acattcaaag cgacctgttc
      601 tacgactatc gaacgaatgt ggacgcctac ccgaagtggc aagcctggat gcgcgagaag
      661 cagccgcgcc tgctggtgct ctgggggaag tatgaccttt cgttcgagct ctcagagccg
      721 gaagcctatc gtcgagacgt gcccaacgct caagtgcacg ttctcgacgc cggtcatttc
      781 gcattggaca cggctgcaga tgagatcgcc gcattggtgc gaggctttgt cgcttcttca
      841 cgttgagggg tcctggcgcg gcacgttggc tgagcccgct ataagaacgc cgacttagaa
      901 tatcacgttc ctgccgcgtt ctctctagtt cccgtttgag tcggcgattt tcttcctcta
      961 acgcagtttg atgcccactg cctggaaagg cttcggggcc gtgttcagct aactctttgc
     1021 gccattgatg gatcgagctg tcagaaatgc ccagctcacg ggcaacctgg gcaatcgact
     1081 tgccactggt ctgagacagc cgcagcgctt cacgcttaaa ctcctgcgta taggtctttt
     1141 gaactttgtg catgatcctg gttctccctc tggaatagta tctcctaaac gttcactact
     1201 ccacaaaagt ggggaaacct cacttacctc tgttggaata acctcgtaga ggtcactgac
     1261 acgtcctttc gcagatgctg cttttctgag aaaccttcgt atgacgaaca tggaagtcaa
     1321 ccctaccagg aagatagccg ggccccaaat gaacattgcg ccaagagcga gtaccagcaa
     1381 ccagtgttgg gcacctctca ggggcagagc agtgaagccc ccggccaggc atggaactcc
     1441 catagcgagt agcataaggg aaagggtcca tcttttcatg agggacctct ggttctttaa
     1501 gcagcgcgac tcagatgatt gtgcgcatcg ctttgtgcgt tctcaacacc aagactccgt
     1561 cgcacctcca ggtaatggac gatggcatcg cggctcaata tgccaaccag ggcgtcattt
     1621 tctagcacgg gcaactgatt cacgtcgcga cctgccatta acgggagcac gtcgctcaaa
     1681 ctctgctgcg gggtcacgac gtgcaagcgc gagagcggga tcatggcagt gctgactggc
     1741 acctgtcccc actgttcacg cggaatatgg cgaatatccc cgagcgttat cagaccgacc
     1801 aggtgatcgg cttgcatcac aagcgcgtag cgcaagccac ctggcagaaa gtaggcgtcg
     1861 accagttgtt gcagcgagat gtcagcaggg acggtcgcgg gtttgggatt catgacctca
     1921 cccacggtca cgccactcaa aacggatgtc agcatgcctt gcgcgttggc tgactgggcg
     1981 gcgcttaata ggaaccaccc gatgaaccca aaccagatgc catccaggat gtcgccaacg
     2041 aagaagatcc agatgccgag caagatgaac aggtaggcga tgacctggcc tgtcagcgag
     2101 gcgacacgcg ttgcttgacg cagattgccg gtgagcctcc atacgatgga acgcagtacg
     2161 cggccaccgt ccagggggaa gccaggaatg agattaaaga cgcccaacaa gatattggtc
     2221 actgccaggt agaacaggat gccttccagc ggggagttgg acccgcgtaa gggcacctgg
     2281 agcaggaaac agatggcgcc aattaccaga ctggtgagag gcccgacgaa ggctatctgg
     2341 aactccacac ccggactctg cggctcacgc tcaatattgg agacgccccc aaagatgaac
     2401 agcgtgatac ttttcacggg caatccacgt ctgcgtgcca ccagcgagtg ggccagttca
     2461 tgcaagagca cggagacaaa cagcagcagg gatgagagga atgcgatgag ccaataggtg
     2521 gcggttgacc atccgggata caactgggga aaccaaccgg tgtcaaggga caccgtcaga
     2581 aagaccagaa taatgatcca gctgacatgg atgtcgatgt cgattccggc aattttgccg
     2641 atacggaacg tgccaggcat tgagatactc cttttctatc tgggttttca tccatttatc
     2701 gggggcagaa cgaattttcc ctccgacaaa tatgagtata ccaggagggc ggggtgaagt
     2761 cagcgtccat gagttggcat ttttcttccc ttacctctca ctggcagaac tgttctcggc
     2821 ctctcgcttc cctggggctt ttcgtgagtt gctgggcctc tcgaccaggc tggctgagtc
     2881 agcataccct gacgaaagcc agtatctcgc tccttgcagc atgatcgaca cggaggaacg
     2941 aagctatgct cttgcttctc tgcccccgaa gttgcgttcg atcgtgcatc tgcgctgttt
     3001 ccggcaactg accttcgcgg aaattgggcg cacactcaac atgccggagc cgactgtgaa
     3061 aacctacttc taccgctctt tgccgctcac gcgcagagcc ctggcagcaa cagcaacctg
     3121 ccatgtgcat tgatctcgta gagggcttcg ttttcacggt tggctccagt ggcttctgcg
     3181 caatcgccca taaccggcgt ggtcgcggcc gctggagccg aagaagtctg atcgtgttcc
     3241 ctgctggtgg agcgtctacc ctggacgttt ccctctcggt aaggagatgg ccagtgggcc
     3301 gggcaaggcc cgttccgcct cgtccgccag gcgctcttcg ttgcggcgca cgatgaagat
     3361 ccccgcaata atgaggagaa cggccagtac gaggagcacg atgccgaccg gcccgaccag
     3421 ccggtgaatg ttgtcaccga ggaagtaccc tcccaggccg tagagcgtgg cccagacgat
     3481 gcctccggcg gcattgaaga gcaggaagcg cggccagcgc atgcggttgg tgccggctag
     3541 aaaggcggcc caggcccgca gaaccgccac gaaccgcccg aagaagacca ctttcccgcc
     3601 gtgcttcagg aacaaatatt ggcccagttt gagccgtcgc tcctctaaat ggatgtagcg
     3661 cccgtagcgg cgcagcaggc ggtagccgcc ctcgcgtccc acccagaagc ccaggttgtc
     3721 gcccaggatg gctccgctgg ccgcggccac gatcacccac gcaatggaga gttgatgggt
     3781 cgtccccgcc gcgatggcgg ccacgagcag aatggtctcg ccagggaagg gaatgccggt
     3841 gctctcgatg gtcacaaaga gcagcaccgc caggtaacca taggtggcca gcaggtgcgt
     3901 cagggttgac gttgccaggt gaaacatagc ctctcctcct ttgcagcgtt ggggccgcat
     3961 ccctagggtt tatccctgga tacccgcttc atcagttcac cgaaccgcat agacttgaaa
     4021 gctgacgcct cctggaatga gcgagcccat gccaatagtg acttcatgct gaatccatgc
     4081 atactgtgat gcactcgtct cgaaatgtgg agtgattacg aagtaatact cctcgttcgg
     4141 gatctgctcc cctgccatcc accggggaag caactccgca accctggtga gatagccccg
     4201 gaacgtctca taaatgagag cgccatcgtc cgtctgcaag gttatccgcg tatcgagttc
     4261 gccaacacca tctgatcgca ccaacagcca ttcgccaacg ccgggaagaa cctcgccttt
     4321 caagcgaggg tccccaccgg gttgccgaaa cgccggtagg caaagcgcac ctcccggaca
     4381 tgcacaaagc gtgtgggagt ggtctcggca ctctctggct gtgtttgctt gtcatgttgt
     4441 atcatccact cgtcctttct ttggatcagc cgaaggtgaa cgcaaacgcc tccacgcctg
     4501 catcgagaaa ctcgatctcg aactggcgat cagcaatatg ccccggttgg cggatcagtt
     4561 gatacagccg ctgctcggtc gccgtgccgt ggccctgctc gtcaacgtca ctgccgggag
     4621 ccgcgcccgg cgcctgtcca tcgaggaaca cgcgaaagcg caccgacgtt cctcgcgctg
     4681 ccggtcccat gaccaggtgc agatcgcggg cgtgaaagcg gtacgcgatg cgcccgttgg
     4741 ccgtgtgcag cgcaacggcc tgttccccca ccgtccactc acccgccagg gcccactgat
     4801 tgagcgagaa gcgcgcgggg gcagtattga cgcgcggctg gtccaggact gccccaccgg
     4861 gagacgcaaa gttctcggtg cgcgcgtccc cgacatagtt ttctggagac ctcaagtcgc
     4921 cccaatcggc ggcagcttca gcaccctggg catcgaccga gaccagttca tgagcgaagc
     4981 cgccgattcc ggcctcggcc agcacctgtt gcagcatcat ttccgactgt tcgtaggcgc
     5041 cctcgccgaa gtgatgagag cgaatacggc cctgcgcatc gacgagatag agggccggcc
     5101 aatagtggtt attgaaagca ctccagatcg catagtcgtt atcgatcgcg atcggatact
     5161 cgacccgcat gttcttcgcg gcttggcgca cgttctcgag gtcgtgctca aaggggaact
//
LOCUS       DASZAK010001112         6399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_28484, whole genome shotgun sequence.
ACCESSION   DASZAK010001112 DASZAK010000000
VERSION     DASZAK010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28484"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VGT44_19730"
     CDS             <1..437
                     /locus_tag="VGT44_19730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583101.1"
                     /translation="GDMIQQMIREESHSSRQLYIPAQDLAAEATTSMSPEAAQVIADS
                     RVRTILQLFNRDYLYGQGRFDEYRDGLLLKWGDGYSRTHIWLSVENGNLLFETSHQKK
                     CEKPYCNGTHHVLSRELYTNLDVVNQELADLFRRPVQEPTED"
     gene            439..846
                     /locus_tag="VGT44_19735"
     CDS             439..846
                     /locus_tag="VGT44_19735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583102.1"
                     /translation="MIILALEKDLFFAVKMRDTLRHHGMEVRTTRTLAAFEQGLQAGA
                     DRQDERPALVIVNTATSGVDWEAAIRQARAAGYPVLAFGSHMDLDARARALEAGAQRV
                     VANSKFTSDMPGLVSRMISAPEAGEPETIESGE"
     gene            complement(827..1045)
                     /locus_tag="VGT44_19740"
     CDS             complement(827..1045)
                     /locus_tag="VGT44_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583103.1"
                     /translation="MSRRIAHRPRYNSTRPTPHAYLRAHHEFLHIRWSYRRQTAPFQK
                     RSPGQSLLNVARIPWSYHLFRGAIPLIQ"
     gene            complement(1174..1575)
                     /gene="erpA"
                     /locus_tag="VGT44_19745"
     CDS             complement(1174..1575)
                     /gene="erpA"
                     /locus_tag="VGT44_19745"
                     /inference="COORDINATES: protein motif:HMM:NF010147.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEV2583104.1"
                     /translation="MIEQTPQSIVETSIITMTTSAADKVRELLTQENDPSLALRIFVA
                     GGGCSGLQYGMTLDEEQDGDTVIPLQGIRVLVDEMSVGYISGSEVDYVDSLMGAGFTV
                     NNPNAVSSCGCGHSFKTAEGGGDARSCGCGH"
     gene            complement(1703..3337)
                     /locus_tag="VGT44_19750"
     CDS             complement(1703..3337)
                     /locus_tag="VGT44_19750"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV2583105.1"
                     /translation="MAAYANELTGIPDGLGYWIPGEMVVVVRLHRHPVAEAQDILVEQ
                     VQRQLNALLAQYGMALEPYGASGRWRDVTGAAKMRRSFIFGRHRQQPLIAIFYQVRQP
                     SLLAQDPMPIALAYIQSNLEQLAQAGLQIVAAMPNWLVTAAPSSYGSGGPALPPVQAP
                     NVTTLEASSALSGWHLSFAEPGFPLDPKGAEDVLIAVLDTARHPGVLQAAATHPDLKR
                     NWLLQQLVSDLRSESGSFEIEYDRFPIISDVCTGQDSQGQPGYYAMPDHGIFVAGVIR
                     DLAPRARIRLIRILNDYGGGDLYNLFAALTDLEQEVVSGAIRRLVINVSLNIMPDIRR
                     LPYIWFDHRQWPSTQLAGAVRVLAHIEEGLRLLFESLHAQGALIVAAAGNDSYEARKQ
                     NQRPRAPRAPARYDTVMSVSSVNSRLAASQFANAACLPPSNVGIATFGGDVYGKLDAN
                     ELPDAVRGLYISSTFPNGEQNRTGWADWSGTSFSAAIISALGAHLMSQSWSASHAMTR
                     IASGRERRTDKLFGPLPDAPELLANVIRVQQSFGLA"
     gene            3568..4668
                     /locus_tag="VGT44_19755"
     CDS             3568..4668
                     /locus_tag="VGT44_19755"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV2583106.1"
                     /translation="MSLDRLTATLIELVGIPSTTSHEEQVRAYLKRHLARLGLRVTID
                     EAGNLVGLLPASAGSGGRPMLLNAHMDRVPPGLGHQPVLRDGALYSDGATNLGADDAA
                     GIVIILEVLRRLVEQSLPHPPVVVVFTVQEEVGLVGASAFDPFAWDVTEGIVFDNAFE
                     AGAVVSRGATYVAFDVELTGHTGHPGKDLSSTVSVIDIFRDAAYPHGSLVGDQTRILV
                     SRIQAGSARNAVPAAAHIEGELRSHEPPEAREGYMQAIAEAFEQAALRHGGSASVTFN
                     PHCTSYSIDEREPLLATYRSVLERRGEKLVMHPSFIGSDTSAFRPRVKTFTVSTGVMR
                     EHSTEEYVELAPLDQIVQDTLQVLRLWGEQTR"
     gene            4665..5354
                     /locus_tag="VGT44_19760"
     CDS             4665..5354
                     /locus_tag="VGT44_19760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583107.1"
                     /translation="MRRAPGQPYQSASRQPNAWNIGRREVIAALFGVILYGFLSHISL
                     IFFVSTNTLDAFLPGLLVPLLFGVIYGPWVGLIVGGFGFLLGDYVASLWLNNLFWDNG
                     YIFVGNFFGSSLANFRDLLGWNGIPGYMANALIGMVAGLATVGARRRYNTLYALALVG
                     ILSTIGITIATAIVDYSAVLIYQSPYYRFNEATLAFFDTALPNLLVALVLLPLLLWGT
                     DALGQWRNGGI"
     gene            5357..>6399
                     /locus_tag="VGT44_19765"
     CDS             5357..>6399
                     /locus_tag="VGT44_19765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2583108.1"
                     /translation="MEQRLGNYHLTRLLARGGFAEVYLGLHVYLNNQAAIKVLLTQLS
                     DHDKASFLNEARTLVNLIHPNIVRVLEFGIEDTTPYLVMDYAPNGSLRQCYPKGERLP
                     LSSVVPYVKQAASALQLAHDQRLIHRDIKPENMLMGRNNELLISDFGIAAFAHSTISE
                     STQQIVGTAAYMAPEQVQGKPRPASDQYSLAIVAYEWLCGERPFHGSLTELATQQLFT
                     DPPPLREQAPDVSPAVEQVIMTALAKDPSQRYGSIQEFANALEFASQSSLQPAMLQPN
                     NPMFSQALVVKSNESAEAVSPSSPTSEVVPPSSPASKEQASVKGLSGIRGGTFFTTQG
                     KRKVLNVTIGVLL"
BASE COUNT         1331 a         1908 c         1910 g         1250 t
ORIGIN      
        1 aaggcgatat gatccagcag atgattcgcg aggagtcgca ctcttcgcgc cagttataca
       61 ttccggcgca agacctggca gctgaggcta cgacaagcat gtcaccggag gcggcgcagg
      121 ttatcgccga tagccgcgtg cgcacgatcc tccagttgtt caatcgcgac tacctgtatg
      181 gtcaggggcg tttcgacgaa taccgcgatg gactgctgct caaatggggc gacggctatt
      241 cgcgcacaca tatctggttg agcgtggaga acggcaattt gctcttcgag accagccacc
      301 agaaaaaatg cgagaagccc tactgcaacg gcacgcacca cgtcctttcg cgtgaacttt
      361 atacgaatct ggatgtggtc aaccaggaac tggccgattt gttccgcagg ccggtacagg
      421 aaccgaccga ggattagtgt gattatttta gccttagaaa aagacctgtt tttcgccgtg
      481 aagatgcgcg atacgctgcg ccatcatggc atggaggtca ggacaactcg cactctggcg
      541 gcctttgagc agggattgca ggcgggcgct gacagacagg acgagcggcc cgcgctggtg
      601 atcgtgaaca ccgcgacgag cggcgtcgat tgggaggccg ccatcaggca ggcgcgagcg
      661 gcagggtatc ccgtgctggc atttggttcg cacatggacc tggacgcgcg cgccagggcg
      721 ctcgaagccg gggcgcagcg cgtcgtggcg aactcgaaat tcaccagcga tatgccagga
      781 ttggtcagcc gtatgataag cgcgccggaa gctggcgagc cggagactat tgaatcaggg
      841 gaatagcccc gcgaaagaga tgatagctcc aggggatacg agcaacattc agtaaactct
      901 ggccgggtga gcgtttctgg aacggagcag tctggcggcg ataggaccag cggatgtgga
      961 gaaattcgtg atgagcgcga aggtaagcgt ggggagtggg tcgagtcgag ttataacgcg
     1021 ggcgatgggc gatgcgtctg ctcattgaag ctcctttatc taaggaacaa cggacacaac
     1081 aaagaggtct ccctcacact atactacgtt tgaggggacc tctttttcac acacatattg
     1141 ctcacaatcg tagctggtca gcaataccgg cgattagtgg ccgcagccgc aactgcgcgc
     1201 atcgccgccg ccttcagcag tcttgaagga atggccgcag ccacagcttg agacggcgtt
     1261 gggattgttg acggtgaaac cagcgcccat caggctgtca acatagtcca cctcgctgcc
     1321 ggagatgtag ccgacgctca tctcgtcgac cagcacgcgg atgccttgca gcggaatgac
     1381 ggtatcgcca tcctgctctt cgtcgagcgt cataccatat tgcaggccag agcagccgcc
     1441 accagcaaca aagatacgca gcgccaggct cggatcgttt tcctgcgtaa gcagttcgcg
     1501 caccttgtcg gcagcggacg tggtcatggt gatgatgctg gtctcaacga tagactgcgg
     1561 agtttgctcg atcatgcgta ttacctcttt caaaagggtt gaagcttcag ccctcaatga
     1621 ttacgtaaat gttcctgagt atgtaatcgg acgtttcgcg tgtttccact tgccagtatt
     1681 atatactaca caaaatgaag cgtcaagcca atccgaagct ttgttggaca cgaatcacat
     1741 ttgcaagcaa ttccggcgcg tccgggagcg gcccgaacag cttgtcggtg cgccgttctc
     1801 ggcctgacgc aatgcgcgtc atggcgtggg acgccgacca gctctgcgac atcaggtgcg
     1861 cgcccagagc gctgatgatg gcggcggaga acgaggtccc cgaccagtcg gcccagccgg
     1921 tacggttctg ctcgccgttt gggaaggtgg acgagatata gaggccgcgc accgcgtcgg
     1981 gcagctcgtt ggcatcgagt ttgccataga catcgccgcc aaaggtagcg atgccaacat
     2041 tcgacggcgg aagacaggca gcgttggcga attgcgaggc ggccaggcgg ctgttgacgg
     2101 acgagacgct catgaccgtg tcgtagcgcg cgggggcgcg cggggcgcgg gggcgctggt
     2161 tttgcttgcg cgcctcgtaa gagtcgttac cggcagccgc cacgatcaag gccccctggg
     2221 cgtgcaggct ctcgaagagc aggcgcagcc cttcctcgat atgcgccagg acgcgcaccg
     2281 cgcccgccag ttgcgtgctg ggccactggc gatgatcgaa ccagatgtag ggcagacggc
     2341 ggatatcggg catgatgttc aggctcacgt tgatgaccag gcggcgaatc gcgcccgata
     2401 cgacctcctg ctcaagatcg gtcagggcgg caaagaggtt gtagaggtct ccgccgccat
     2461 agtcgttgag gatgcgaatg agccggatac gcgcgcgcgg agccaggtcg cggatcacac
     2521 ccgcaacgaa gatgccgtga tcgggcatag cataatagcc gggctgaccc tggctatctt
     2581 gcccggtaca gacatctgaa atgatgggga agcgatcata ttcgatctcg aacgaaccac
     2641 tttcgctgcg caagtcggag acgagttgtt gcagcagcca gttccgtttc agatcggggt
     2701 gtgtggcggc tgcctgcaat acgccggggt gccgtgccgt atccagcacg gcgatcagca
     2761 cgtcttcagc gcccttcggg tcgagtggaa agcctggctc agcgaaagag aggtgccagc
     2821 cagaaagagc gctgctcgct tcgagggtgg tcacattagg ggcctgaacg gggggcagcg
     2881 ccgggccgcc gctgccatag ctggacggcg cggctgtcac cagccagttc ggcatggcag
     2941 ccacgatttg cagtcctgcc tgggcaagct gctccagatt actctggatg taggccagcg
     3001 cgatgggcat ggggtcttga gccaggaggg agggttggcg cacctggtag aaaatggcga
     3061 tgagcggctg ctggcgatgc cgtccaaaaa tgaacgaacg ccgcatcttc gccgcgcctg
     3121 ttacatcgcg ccaacgcccg gaggcaccgt atggctccaa tgccatccca tattgcgcca
     3181 gcagcgcatt cagttgtcgc tgtacctgct cgaccaatat atcctgcgct tcggccacag
     3241 ggtgccggtg cagcctcact accactacca tctcgccggg tatccaatac cctaacccat
     3301 cgggtattcc cgtcaactca ttcgcataag cggccatgca cgtactcccc cctatccgct
     3361 ctgtgatgaa agatgcgcga gtcgctgcga ggcgctaacc gctcgccaat atgatgtaaa
     3421 ataagcagta gcgattgttt ctgcttatgc gcattataca ggaacgtcta tacttgtggc
     3481 aacccgatga agtagatagc ggcgagcttg gcgatccctt gcggtcgcca tgctcgccaa
     3541 cgaacacaac ctggaaggta aacatacatg tcactcgacc ggctcacagc cacgctgatc
     3601 gaactcgtcg gcattccgag tacgaccagc cacgaagagc aagtgcgcgc gtacttgaag
     3661 cggcacctgg cgcggctggg tttgcgcgtc acgatagacg aggcgggcaa cctagtcggg
     3721 ctgctcccgg ccagcgcggg cagcgggggc aggccgatgc tgctcaacgc gcacatggat
     3781 cgcgtaccgc cgggcctcgg ccaccagccg gtgctgcgcg acggcgctct ctatagcgat
     3841 ggcgcgacca acctcggcgc ggacgacgct gccggtatcg ttatcatcct ggaagtgctg
     3901 cgacggcttg tcgagcaaag cctgccccat ccgccggtgg tggttgtatt tacggtgcag
     3961 gaagaggttg ggctggtggg cgcatcggcc ttcgaccctt tcgcctggga tgtcaccgag
     4021 ggcatcgtct tcgacaacgc ctttgaagcg ggcgcggtcg tgtcgcgagg cgcaacctac
     4081 gtggccttcg atgtggagct gaccggccat accgggcatc ctggcaaaga cctgtcctcc
     4141 actgtcagcg tcatcgacat ctttcgcgac gcggcctacc cgcatggctc gctggtcggc
     4201 gaccagacgc gcatcctcgt cagccgcatc caggcaggca gcgcgcgcaa cgccgttccc
     4261 gccgccgcgc atatcgaggg cgagctgcgc agccacgaac cgccggaagc gcgcgaaggc
     4321 tatatgcagg ctatcgcaga ggcgttcgag caggccgctt tgcggcacgg tggcagcgcc
     4381 agtgtgacct tcaacccaca ctgcacgtct tacagcattg atgagcgcga accgctgctg
     4441 gctacctatc gctccgtgct ggaacgacgc ggggagaagc tggtcatgca tccgtccttc
     4501 atcggcagcg atacaagcgc gttcaggccg cgcgtcaaaa ccttcaccgt atcgaccggc
     4561 gtcatgcgcg agcattcaac ggaagaatac gtcgaacttg cgccattgga tcaaatcgtg
     4621 caggacacat tacaggtcct gcggctttgg ggagagcaaa caagatgaga agagcgcccg
     4681 gacaaccata ccaatccgcc agcaggcagc ccaacgcctg gaacattggc aggcgcgagg
     4741 tgatcgccgc tctgttcggg gtaatcctct atggctttct cagccacatc agcctgatct
     4801 tttttgtctc gaccaatacc cttgatgcct tcctccccgg actgctcgtt ccgctgctat
     4861 tcggcgtgat ctacggcccc tgggtcggcc tgatcgtggg tggctttggc tttctattgg
     4921 gtgattatgt cgccagtctc tggctgaaca atctcttctg ggacaatggc tacatctttg
     4981 tcggcaactt cttcggcagc tcgctcgcta actttcgcga cctcctcggt tggaacggta
     5041 tccccggcta catggccaat gccctcatcg gcatggtcgc ggggctggct accgtcggcg
     5101 cgcggaggcg ctacaacacg ctctacgcgc tggcgctcgt cggcatcctc tctacaatcg
     5161 gcattaccat cgctaccgcc atcgtcgact acagcgccgt actgatctac cagtccccct
     5221 attaccgctt caacgaagcc acgctggcct tcttcgacac cgcgctgccg aacctgctgg
     5281 tcgcgctggt gctgctgcca ctgctgttgt gggggaccga cgcgctggga cagtggagaa
     5341 atggtgggat ctgagtatgg agcaacgact cggaaactac cacttaaccc gcttactggc
     5401 gcgcggaggc ttcgctgaag tctacctcgg cctgcacgtg tacctcaaca accaggccgc
     5461 catcaaagtc ctcctgacac agctttcaga ccatgataaa gcaagctttc tcaacgaagc
     5521 gcgcaccctg gtcaatttga tccaccccaa cattgtgcgc gtgcttgagt tcggcatcga
     5581 ggatacgact ccctacctgg tcatggacta tgcgccgaac ggctcgcttc gccagtgtta
     5641 tcccaaaggc gaacgactcc cactatcgag tgtcgtaccc tatgtcaaac aggccgccag
     5701 cgcattgcag cttgcccacg atcaaaggct cattcatcga gatatcaagc cggaaaatat
     5761 gctgatggga cgcaacaacg agctgctcat cagcgatttt ggcatcgccg cttttgcgca
     5821 tagcacgatc tctgaaagca cgcagcagat cgttggcacc gcggcctaca tggctcctga
     5881 gcaggtgcag ggcaagccgc gcccggccag cgaccagtat tccttagcca tcgtggctta
     5941 cgagtggtta tgcggagagc gaccctttca cggcagcctg acagagttgg ccacccagca
     6001 gctatttacc gacccacccc cgctgcgcga gcaagcgccg gatgtctctc ccgctgttga
     6061 acaggtgatt atgaccgcgc tcgccaaaga ccccagtcag cgctatggct ccatccagga
     6121 gttcgccaat gcactagagt tcgccagcca gtcctctctg caaccggcca tgcttcagcc
     6181 aaacaaccct atgttttctc aagcgctagt ggtaaagagc aacgaatccg cagaagcagt
     6241 atcaccttcc tccccgacga gcgaggtagt gccgccttct tctccagcga gcaaagaaca
     6301 ggccagcgtc aaagggcttt ctgggataag aggcggaacg ttcttcacca ctcaaggcaa
     6361 acgcaaggtg ctcaacgtga caattggggt gctgctcta
//
LOCUS       DASZAK010001113         1507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_597054, whole genome shotgun sequence.
ACCESSION   DASZAK010001113 DASZAK010000000
VERSION     DASZAK010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597054"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1416
                     /locus_tag="VGT44_19770"
     CDS             <1..1416
                     /locus_tag="VGT44_19770"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEV2583109.1"
                     /translation="RKPANRPSSPDAPAHTTRANQPSRPATVAPSRRSGTVTRSIQTW
                     RERPRPLFTRKRLATGAGVTVLLLLLLVPLLLRASHFGASSDGEIVTTDSTQIAGVGA
                     QPTPDHNLIVKPQDLDHPAPPVMAASAYLLDANTGNTLYAYNAFEHLPMLSTTKLMTA
                     ILALEQQGSNLDQRITISAAMSNDISKLSPDSALFGIKKGEIYTLRDLLYGLLFVSGN
                     DAAIAIADALAGDMQHFVDEMNAKAQQLGMLDTHFVNPHGLLQNGQYSSAHDLALLGR
                     FAMTIPTLHSISGNKAYYIPAGGNHPQRYLLNENQFLWWYPGVDGGKTGYDGASDFIQ
                     VMSVTRNNHHLIGVVMDTNNWWTDMRDLMNWGFDTFAWVSPALVDAQHPIPYDSLWNY
                     FAKDTINTTIPTADKGRYYIYTGFEINGPIMAYFDAHGGLKSFGYPISLTKTSNSTQI
                     SQQFQNATIQCGLSKLSCQKV"
BASE COUNT          342 a          514 c          384 g          267 t
ORIGIN      
        1 agaaagccag cgaaccgacc gtcgtctccc gatgcgcccg ctcacacaac gcgcgccaac
       61 cagccgtcgc ggcctgcgac agtcgcgccc agccggcgat cagggacggt cactcgctca
      121 atccagacat ggcgcgagcg acctcgacca ctcttcaccc gcaagcgctt ggcaacgggc
      181 gcaggcgtca ccgtcctgct gctgctcctg cttgtccccc tgctcctgcg agccagccac
      241 tttggcgcat cgtcggacgg cgagattgtc accaccgaca gcacacaaat tgcaggggtc
      301 ggcgcgcagc caacgccgga tcacaatctg atcgtcaaac cccaggacct cgatcatcca
      361 gcgccgcccg tgatggctgc ctcggcctat ctgctcgatg ccaataccgg caacacgttg
      421 tatgcctata acgccttcga gcatctcccc atgctcagca cgaccaagct catgacggcc
      481 attctggccc tcgaacagca gggcagcaat ctcgatcagc gtatcacgat cagcgcggcc
      541 atgagcaatg acatcagcaa gctcagtcca gacagcgcgc tcttcggcat aaaaaaggga
      601 gagatctaca ccctgcgcga tctgttgtat ggcctgctct tcgtctccgg caacgacgcg
      661 gccatcgcca tcgccgatgc gctggccggc gacatgcagc actttgtgga cgagatgaac
      721 gcgaaggcgc agcagcttgg gatgctcgat acccatttcg tcaatccgca cggcctcttg
      781 caaaacggcc agtattccag cgcgcacgac ctggcgctgc taggccgctt cgccatgacc
      841 atcccaacgc tgcatagcat cagtggcaat aaagcgtatt acatcccggc aggcggcaac
      901 cacccgcagc gctatcttct caacgaaaac cagtttctct ggtggtatcc cggtgtggat
      961 ggcggcaaga ccggctacga cggggcatcg gatttcatcc aggtcatgtc ggtgacgcgc
     1021 aacaatcatc acctcatcgg cgtcgtcatg gatacgaaca actggtggac agatatgcgc
     1081 gacctgatga actggggctt cgacaccttc gcctgggtct cgccagccct cgtcgacgcg
     1141 cagcatccca tcccctacga cagcctgtgg aactacttcg cgaaggatac catcaacacg
     1201 acaatcccca ccgcagacaa aggacgctac tacatctaca ccggcttcga gatcaacggc
     1261 ccaatcatgg cctatttcga cgcgcacggc ggcctgaagt cgttcggcta cccgatcagt
     1321 ctgacgaaaa catccaactc gacccagatc agccagcaat tccagaacgc caccatccag
     1381 tgcggcctct caaaactatc gtgccaaaag gtctaatgag gaataggatc acaaccccag
     1441 taggagccgg tttaccgtgc tcgcagccga ttgatcggca tgaatccctc gtcgtcgtca
     1501 aaaggct
//
LOCUS       DASZAK010001114         3621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_204130, whole genome shotgun sequence.
ACCESSION   DASZAK010001114 DASZAK010000000
VERSION     DASZAK010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204130"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VGT44_19775"
     CDS             <1..919
                     /locus_tag="VGT44_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583110.1"
                     /translation="ELARENRAYSDIASKFFEHFVYIAHAMNEMGDEGFSLWNEEDGF
                     YYDVLHVEGAGHLPLKVRSMVGLIPLFAISILEADLVDKLPRFKRRMNWFIQNWEDIS
                     EHIEQSQTDGHNDHLLLSIVNREKLPRILCVMLDEAEFLSHYGIRALSRYHLEHPYML
                     DIGGINNRIAYEPAESSSGLFGGNSNWRGPIWFPVNYLIIESLRTFDHFYGETLKVEF
                     PTGSAHWLTLAEVADELATRLKRIFLRDEQGHRAVFGGAELFQRDPHWRDLLLFYEYF
                     HGDNGAGIGASHQTGWTGLIANLFQQHMI"
     gene            962..1459
                     /locus_tag="VGT44_19780"
     CDS             962..1459
                     /locus_tag="VGT44_19780"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HEV2583111.1"
                     /translation="MHIKTDARIFISLGAKQALLLDVSARRTIEACGVLIGSIDEAGN
                     WHVESIHPLRNIFDSPTYFEFAPEELLAADLAYPGQVIGVYHSHPTGFAKPSQTDRQN
                     MKRVNISQRIPWAWLIVCGPFQTSSLDTKQAEPAFKAEATILAFHHYADDGLCSIPLL
                     IAPEV"
     gene            1602..2726
                     /locus_tag="VGT44_19785"
     CDS             1602..2726
                     /locus_tag="VGT44_19785"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2583112.1"
                     /translation="MRTPLSHRILPPTRFKRAQMLFALISLVVFLAACGSSPTTTGTT
                     PSSGGGTTSTSPTCDSSAGLTLYSAQGYDSDAAKAFQQQTGITTNLVDDSTGPLLAKI
                     AAEGNNPQWDVAWFDGNVTMQSLDDQGLLLKWQSPNLSNLTAQGMKYVSSDYSYYPSG
                     LTTVGAIAYNKNHIPAAGLPQDWSDLLKPAYKNLVAMNDPAYSGPTYPLIAGVAQLLG
                     GENQGKQYFTQLKANGLKIFQTNDPTLNSVETGAREFGIVQDSAIYGAIHSGQPLGII
                     YPTSGVIGLPGVIGISANAPHKGCAEKFVNWVLSNAGQTVMVHHDITDGDRYFIPVVQ
                     GITNTANREYSGINFIDLNIPQWATAENEYKQWFHANIVQ"
     gene            2699..>3621
                     /locus_tag="VGT44_19790"
     CDS             2699..>3621
                     /locus_tag="VGT44_19790"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2583113.1"
                     /translation="MVPRQYRPVGVVGEQEQGIADIAAIPSSPSFWKHLLQRLLQGSV
                     IVLPVLLLALLILYPLAAIILQSVFPNLFAASPNLIPSWSALQEVVQNSQNYLALFNS
                     MWLAGITALLSCIIGASLAILSRRTNMPLARFLDIPVWIVFFTPSFLIGEAWSLTLIR
                     GGIPDQYLHFSDGFINWFFSPVGVVFILSLKSFPYVYFSVAAALRWLGSEFEDAARIS
                     GARGWRAWLSINAPLLLPAILAAGLIAFAEALSDFGTAATIAQNANVTLVTYQIYAAI
                     NTAPVDFGLAAALSLLLFIAIAVALLVQAGIL"
BASE COUNT          748 a         1154 c          935 g          784 t
ORIGIN      
        1 ggaactggca cgcgaaaacc gtgcctacag cgatatcgcc agcaaattct tcgagcattt
       61 cgtctatatc gcccatgcca tgaacgagat gggagacgag ggtttctcgc tctggaacga
      121 agaggacggc ttctattacg acgtgctgca tgtcgaaggc gcagggcatc tgccgctcaa
      181 ggtgcgctcc atggtgggat tgattccgct cttcgccatc tctattctgg aggctgacct
      241 ggtcgataag cttcccagat tcaagcggcg catgaactgg ttcatccaga actgggagga
      301 catctctgaa catatcgagc aatcacagac cgatgggcat aacgatcacc tgctactttc
      361 gattgtgaac cgcgagaagc tgccgcgcat cctgtgcgtg atgctggatg aagcggagtt
      421 cctctcgcac tacgggattc gcgcgctgtc ccgctatcac ctggaacacc cctacatgct
      481 ggatatcgga ggcattaaca accgcatcgc ctatgaacct gccgagtcgt cctccggcct
      541 gttcggaggc aattccaact ggcgcggacc gatctggttc cccgtcaact acctgattat
      601 cgagtcgctg cgcacctttg accacttcta tggtgaaacg ctcaaggtgg agtttccaac
      661 cggttcagcc cactggttga cgcttgccga ggtggcagat gagctggcca ctcgcctgaa
      721 gcgcattttc ctgcgagacg agcagggaca cagggcagtc ttcggcggcg cggagctatt
      781 ccagcgcgac ccgcactggc gtgacctgct gctcttctac gagtactttc atggcgacaa
      841 cggcgcgggc atcggcgcga gccaccagac aggctggacg ggcttgatcg ccaacctgtt
      901 ccagcaacat atgatatgat aaagcatcat caacggtacg aagggatatt ccatcgctca
      961 tatgcatatc aagacagatg cgcgcatttt tatcagcctg ggcgcgaagc aggcgctgtt
     1021 gctggacgtg tctgcacggc gcaccatcga agcctgtggc gttctgatag ggagcatcga
     1081 tgaagcaggg aactggcacg tggaaagcat tcatccacta cgcaacatct tcgattcgcc
     1141 tacatacttt gaattcgccc ccgaagagct gctggccgcc gacctggcct accccggcca
     1201 ggtcatcggc gtctatcaca gccatccgac cggcttcgcc aaacccagcc agaccgaccg
     1261 ccagaatatg aagcgcgtca acatcagcca gcgcatcccc tgggcctggc tgatcgtctg
     1321 cgggccattc cagacttcgt cgcttgacac aaaacaggca gaaccagcgt tcaaggcgga
     1381 agcaacgatc ctggccttcc atcattatgc cgacgacggc ttatgcagca tccccttgct
     1441 tattgcccca gaagtttaac aagctttcac aatccgatta cattccgtta atcttctctt
     1501 gctcaacgct taatacgcgc tttctatact ctctaataag caggagcatt aacatgtccc
     1561 tgcctcgttg tgaacaccgc gaccaaggag agtattttct catgcgcaca cctctgtctc
     1621 atcgtatctt gccaccgacg cgcttcaaac gcgcgcaaat gttgtttgca ttgatcagtc
     1681 tcgttgtatt tctggccgcc tgcggcagca gcccgaccac caccggcacc acaccttcca
     1741 gcggcggagg gacaacttca acctcaccta cctgtgatag ctcggctggc ctgacgctct
     1801 actcggcgca aggctacgac tcggatgccg ccaaagcctt ccagcagcag accggcatta
     1861 cgaccaacct ggtcgacgac agcaccggcc ccttactcgc caaaatcgcc gccgaaggca
     1921 acaaccccca gtgggatgta gcctggttcg atggtaacgt caccatgcag tcgctcgacg
     1981 accaggggct gttgttgaaa tggcaatcgc ccaacctgag taacctgacc gcgcagggca
     2041 tgaaatacgt gtcgagcgac tacagctatt acccctccgg cctgacgacg gttggcgcca
     2101 tcgcctataa taagaaccac ataccggcag ccgggctacc gcaagattgg agcgacctcc
     2161 tcaaaccggc ctacaagaac cttgtcgcca tgaacgaccc ggcctattcg gggccaacct
     2221 atcccttgat cgccggcgtc gcgcagcttc tgggcggcga aaaccagggc aagcagtact
     2281 tcacccagct taaagcgaac gggctgaaga tcttccagac caacgaccct acgctcaaca
     2341 gcgttgagac cggcgcgcgc gaattcggca tcgtgcaaga ctcggccatc tacggcgcga
     2401 tccattcggg ccagccgctg ggcatcatct accccacttc gggcgtgatc ggcctgcccg
     2461 gcgtcatcgg tatctcggcc aacgcgccgc ataaaggctg cgccgagaag ttcgtcaatt
     2521 gggtgctgtc caacgccggc cagaccgtca tggtccacca cgacatcacc gacggcgacc
     2581 gctacttcat tccggtggta cagggcatca ccaataccgc caatcgcgag tacagcggca
     2641 tcaacttcat cgacctgaac atacctcagt gggctacagc ggagaacgag tacaaacaat
     2701 ggttccacgc caatatcgtc cagtaggtgt tgtcggagaa caggagcagg gtatagcgga
     2761 tatcgccgct ataccctctt cgccatcttt ctggaaacac ctgctacagc gcctgctcca
     2821 gggttcggtg atcgttcttc ccgtcctgct gctcgcgctg ctgattctct atcccctggc
     2881 ggccatcatc ctgcaaagcg tcttcccaaa cctgtttgcc gcctcgccaa atctgattcc
     2941 gagctggtcg gcactgcaag aagtcgtaca gaattctcag aattacctgg cgctcttcaa
     3001 ctcgatgtgg ctggcgggca tcactgcatt gctaagttgt atcatcggcg cttccctggc
     3061 gatcctgtca cgccgcacca acatgccgct cgcccgcttc ctcgatattc ccgtctggat
     3121 cgtcttcttc acgccctcgt ttctgatcgg cgaggcgtgg tcgcttactc tcatacgcgg
     3181 cggcattccc gaccagtacc tgcatttctc cgatggcttt atcaactggt tcttttcgcc
     3241 ggtcggcgtc gtcttcatcc tgagtttgaa aagctttccc tatgtctatt tctcggtcgc
     3301 ggcggcgctg cgctggcttg gctcggaatt tgaagacgcg gcgcgcattt cgggcgcgcg
     3361 aggttggcgc gcctggctca gcatcaacgc gcccttgcta ctgcctgcca ttctcgcggc
     3421 tggcctgatc gccttcgccg aagcgttatc ggatttcggc acagccgcta ccattgcgca
     3481 gaacgcgaac gtgacgctgg tcacgtacca gatttacgcc gccatcaata ccgcccccgt
     3541 cgattttggc ctggctgccg cgctgtccct gctgctcttt attgccattg ccgtcgctct
     3601 gctcgtgcag gcgggcatcc t
//
LOCUS       DASZAK010001115         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_100902, whole genome shotgun sequence.
ACCESSION   DASZAK010001115 DASZAK010000000
VERSION     DASZAK010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100902"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..1450
                     /locus_tag="VGT44_19795"
     CDS             422..1450
                     /locus_tag="VGT44_19795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583114.1"
                     /translation="MEERLLLYLEYHPQVAWYGRGDISPTFASTYKLTTPGPVPFTIS
                     YLFEGKAHGYLPDAIGQLLDGRLLIAEAGMQQDKRRARNRAKAEAARKVAEQQAGVFW
                     IGTEATLSPVRHANLVFLHARRQSFPAWQELEEALQVIWPWGEVACVQELVERLGERW
                     PAAEREAAIWKRCAESAASGHLLVDLGSVSLSRLTPLLCLSPDEAAILPDPLQEDVTE
                     SSETALVGSEEEAEPEPSARQSLSSTFDASTLPDSKRERFLRNLRAVEAVLAGATVVE
                     TAQETGVACSTLSRLVRRTRELGALACVPYATYSRERALHPAFQEVIHLLYTRPSRLS
                     LRAKALAC"
     gene            1674..1916
                     /locus_tag="VGT44_19800"
     CDS             1674..1916
                     /locus_tag="VGT44_19800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583115.1"
                     /translation="MELYVITPDGIPVTRRVHAAVLVCVKTAAIMSAVLALGPLKEED
                     YMRPDLGGTGTQGSPGPASRVSAPLALFRQASSRLS"
     gene            1954..2856
                     /locus_tag="VGT44_19805"
     CDS             1954..2856
                     /locus_tag="VGT44_19805"
                     /inference="COORDINATES: protein motif:HMM:NF020859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HEV2583116.1"
                     /translation="MLVDRLGIITEQAPPYCPSAKGTVEALFRWMTQRFERRLPNTSH
                     GSHSAEAAAQAGAMTLEDLEGYFLRAIVDDYQQSWDGLRRQIRTVLWEEAVRHTGVPQ
                     YLGAPDDLKLLLMKAANRKARSHSYRVHDGSRLSFQGRWYVCPGLLNRLQGKEFELYY
                     DRRDISVIYLFVEGRYVGEAYCPALMGRRVSEWEATAQRKNDSQRAKAAASASQEVRA
                     RIQEEIETAKQRRRRLTREQEYSRQLDRQREEIHPPHVLDQLAQMTPPAPSSLRLPEA
                     KPDPKGDYPVQLLAIRPFDQENDS"
BASE COUNT          634 a          907 c          884 g          587 t
ORIGIN      
        1 ccgcttctag agccatcttc ccagattcga atcagtcgat tccaatgatt gacccaaatt
       61 acccaccatt gacctcaaaa acggctcttg ctcaattccg gtagaggtcg cttgcttgga
      121 aagtggtaaa taggctgaag gttcgatcga atcagaccca atgatcgaat tatggaccca
      181 aggttcgaac agaaacggct gagcggccct tagacgagct tgtcccctga gtatactgct
      241 cttgaagagg tggaaattcg cctctagtgc attgagcgac accgaaaggg ggcatggcat
      301 gcctgagacc acagttcgtg gcattgactt attcaaaatt gccggagaga gcatctcggg
      361 atggatcccg agctttaaaa cgggagaggc ccggccgact cggcaaccct tctgtagtca
      421 gatggaagaa cgactcctgt tgtacctcga atatcacccg caggtggcct ggtatgggcg
      481 cggcgatatc agccccacat ttgcttcgac ctacaagctc accactcccg ggccggttcc
      541 cttcaccatc agctatctct tcgaaggcaa ggcccatggc taccttcccg atgccatcgg
      601 ccagctgctc gatggtcgcc tgctgatcgc agaagccggg atgcagcaag acaaacggag
      661 ggcccgcaat cgagccaaag ccgaagcggc gcgcaaagtc gccgagcagc aggcaggggt
      721 cttttggatt ggcactgaag cgaccttgtc gccagtcagg cacgccaatc tcgtgttttt
      781 gcatgcccgg cgccagtcct ttcctgcctg gcaagagctg gaagaggcgc tgcaagtgat
      841 ttggccctgg ggcgaagtcg cctgcgtgca agagttggtc gaacgcctgg gagaacgctg
      901 gcccgcggct gagcgagaag cagcgatctg gaagcgctgt gccgagagcg cggcatcagg
      961 tcatctgctg gtcgatctcg gctccgtctc cctcagcagg ctgactcctc tgctgtgtct
     1021 ttcccctgac gaggcagcca ttctcccaga ccctctgcaa gaggacgtga cggagagcag
     1081 tgagacagca ctggtaggct cagaggaaga ggctgaacca gagccttctg cgaggcagag
     1141 tctctcgtcc acttttgatg cctccactct cccagacagc aagcgcgagc gctttctgcg
     1201 caatctgcga gccgtcgaag cggtcctagc cggggctacg gtcgtcgaga cagcacaaga
     1261 aacgggagta gcctgctcga cgctctcgcg tctagtgcga cgcacccgcg aactgggtgc
     1321 gcttgcctgt gtcccgtatg cgacctacag ccgagagcga gcactgcatc ctgcctttca
     1381 agaagtcatt catctcttgt acactcgtcc gagcaggctc tcgctacgag ccaaggctct
     1441 cgcatgctga actcaagcgg gtcgcttccc gcctccagca agacacaggc acggccatcg
     1501 cgctgccttc ctacgaccag gtccgggcct atgtacggac cctcaagcag gaaccgaagg
     1561 tacgaggggc acgcgaagcg ctgcccagac ctgggcgcga gcggcagtcc ccacgctcct
     1621 ttgcgctctc cattccagca ccggcacaac tggtccaggt ggatgaacat agcatggaac
     1681 tctatgtcat tacgccagac ggcattcctg tcacgcgtcg tgttcatgcc gccgtgctcg
     1741 tgtgtgtgaa aacagccgcc atcatgagtg ccgtgctggc gttggggcca ctcaaagaag
     1801 aagactacat gcgacccgat ctcgggggca ctggaaccca aggatcgcct ggtcctgcaa
     1861 gccgggtgtc agcacccctg gccttgttcc ggcaagccag cagtcgtctt tcatgaccgg
     1921 ggcaaaatct ttacctccga acgagcccgg catgtgctgg tggatcgact cggcatcatc
     1981 accgagcaag cgccgcccta ttgtccttca gcgaaaggca ccgtggaggc cctctttcgt
     2041 tggatgacgc aacgctttga gcggcgtttg cccaatacca gtcatggctc acattctgcc
     2101 gaagcggcgg ctcaagcagg ggcgatgacc cttgaggacc tggaggggta ttttctgcgt
     2161 gcgatcgtgg acgattacca acaaagctgg gatgggctgc gtcgtcagat ccgcacggtg
     2221 ctctgggagg aagccgtgcg ccacaccggc gttcctcagt atctgggggc gcctgatgac
     2281 ctcaagttgc tgttgatgaa ggccgccaat cgcaaagcgc gcagccacag ctaccgggtc
     2341 catgatggct cacgcctctc ctttcaaggg cgctggtatg tgtgccctgg cttactcaat
     2401 cgactgcaag gcaaggagtt cgagctctat tacgatcgac gggatatctc ggtcatctac
     2461 ctgtttgtcg agggcaggta tgtgggggag gcctactgcc cggccctgat gggcaggcgg
     2521 gtgagtgagt gggaagcgac agcccagcga aagaatgact cccaaagggc aaaagccgct
     2581 gcctccgctt cccaggaggt ccgagcgcgc atacaagaag agattgagac ggccaagcag
     2641 cggcgcaggc ggctgacgcg cgagcaggaa tactctcgcc aactcgatcg ccaacgcgag
     2701 gagatccacc cgccgcatgt gctggatcag cttgcccaaa tgacgccccc tgcgccttcc
     2761 agtttgcggc tcccagaggc caaacctgat ccgaaaggcg actatcctgt gcagctgctg
     2821 gccatccgtc cctttgatca ggagaacgac tcatgaagcg accgactctg ccactcttcg
     2881 aaggctttat cgagacccct ttcgaacagc gttttcaggg gctcttgcgc caggcgtgga
     2941 agctgaggcg ttggcatgtc attgtcgcag aaccaggcag cggcaaaacc atgggcatcc
     3001 gcgatgtgag cg
//
LOCUS       DASZAK010001116         5457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_240824, whole genome shotgun sequence.
ACCESSION   DASZAK010001116 DASZAK010000000
VERSION     DASZAK010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5457
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240824"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VGT44_19810"
     CDS             complement(<1..242)
                     /locus_tag="VGT44_19810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583117.1"
                     /translation="MPGKVRITIGIVTAVAVVMALVLGLHVGGKAVLRIGLYSQLAGA
                     FIGGFLAIISVNFPLRRGEQIEPWLRRERLAWTLIGA"
     gene            529..1485
                     /locus_tag="VGT44_19815"
     CDS             529..1485
                     /locus_tag="VGT44_19815"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV2583118.1"
                     /translation="MSSKRLSVIPRGVYPPLPTFFDEQEELDLATFQRHIANLAHTGI
                     AGCVVMGSNGEAVHLTADERVQVIEAAREMVAGETPLIAGCGEQSTRSTIANCRRAAQ
                     AGADAALILPPVYYKSQMNRRALVAHYQALADSSPLPIVIYNMPANAAGLDLDADTVC
                     ILAEHPNIICIKDSAGDMVKLAQIVACVPATFHVFAGSAGYFLPALSVGVVGVVAALA
                     NIFPRQVCRVQELFDAGRMEQARDLQAALVAANSAVTSIYGVPGLKAALELTAGQEGH
                     LPAHCGILRSPLQPLTELERARLQEIIKGVPRIEEWDIIKSE"
     gene            complement(1486..2589)
                     /locus_tag="VGT44_19820"
     CDS             complement(1486..2589)
                     /locus_tag="VGT44_19820"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583119.1"
                     /translation="MFTALRSAWIIMSKDIAVWLRQPVNIAVTLLPPLGFLLVAALGS
                     AAVGRSPVALVTLDSGAKGQQMAQIIHNADVFRLTDATPTQAQVLLNNIDVVAIITIP
                     ADFTQRVDAHLSAPINVTVNNLNLDFTNDIRRSVPDAITQYYQAQGSSSPVLVTMHET
                     NLRSRDVQLFQYDVLPTILLLLTISGLINGGLSTAREWERNTVKELLLSPTARGAIIA
                     GKVLAGFAITFVLGTLVLLLGDLLGWTQPEGIYWVNALLIIALVSLFSAGVGVAIGAA
                     LRRIQVVIAVSINVALYLFFLAGGIGVLAFEPGWLQTIAAFVPLTFGRHALEQAIFYS
                     ASDQLGLDITVLAAFTLAALVLGTLSMRRGIAR"
     gene            complement(2676..3791)
                     /locus_tag="VGT44_19825"
     CDS             complement(2676..3791)
                     /locus_tag="VGT44_19825"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583120.1"
                     /translation="MFQRTKYDLRVVWACMKKDITSALTERVFTIISIFVPVNILILL
                     SLFVVSGGQAPTAVVMNDSGPYAQQFYNAMSHAHSFVLQTASASDAQNLITTGKIVAV
                     VTIPADFDARIRQNQPVQVGVQINNLNTDFTNDIRRAIPLSITSFYAKAFPSLVNITP
                     SESDLQPQDTDYIPYLTVSILVIGLMLGGLLQSGTSTAREYENETIKELLLSPASRWA
                     VIVGKMLGAFVMSISAVIVVLLVLIVIIGVRPDHWGEVVGFTLLAMLLFIALGAVLGT
                     LIKNRQPVIALSFGVSIPLFMLSGAFGPISFTTPAIQVVAQIFPIYYAIVLQQHAFHN
                     FILNTYGLAANVLILGAYALGLVLIAAFVLRRSTLAH"
     gene            complement(3838..4824)
                     /locus_tag="VGT44_19830"
     CDS             complement(3838..4824)
                     /locus_tag="VGT44_19830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583121.1"
                     /translation="MTQEITQAGASTGTGKKDGVPAIEMQDLCKNFGTFRAVDHLSLS
                     VRQGEIFGLLGPNGSGKTTTINMISGLSIATSGEVRVMGYDVRHESRKIRGMLGSVPQ
                     ETALYDELTAWANMDFHANLFNIPRKEKKERITSLLTLVELLDRKDSRVGTFSGGMKR
                     RLALARALLHDPHLIYLDEPTLGVDVQARRAIWDYILALRDQGKTVLITTNYLEEAQA
                     LCERLAIIDHGKLIAVDTPDHLKQTYGGSIIEVETARPTQQAVEAIRHLPGVTHIDQE
                     GARLKITTSGSDGNVAQIVNILAQESDIRDIAIREPNLDEIFLLLTGKALRD"
     gene            complement(4852..5358)
                     /locus_tag="VGT44_19835"
     CDS             complement(4852..5358)
                     /locus_tag="VGT44_19835"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV2583122.1"
                     /translation="MTFQAEDFLGYWLFRAQRSVVYAFYEALKACCQEYDKPYVVTPP
                     QFGVLSLLDEQRGMTIGAISQKRGVDAATITGIVTRLEQSGLVERRHDRKDRRQVYVF
                     LTSEGSDIMRYLPDVAAALTGTLMKGFTQDEQQDLIAKLQQILANLSAVEPDPKVGAD
                     LLPNFREG"
BASE COUNT         1185 a         1645 c         1530 g         1097 t
ORIGIN      
        1 gcgccgatca gcgtccaggc cagtcgctcg cgtcgcaacc agggttctat ctgttctccg
       61 cggcgaagcg ggaaattcac tgagatgatg gccagaaacc caccaatgaa ggccccggct
      121 aattgagaat aaagcccgat gcgcagaacc gccttaccgc caacatgcaa ccccagcaca
      181 agagccatca ctaccgctac cgccgtgact ataccaatcg taattcgcac ttttccaggc
      241 atgttgttcc tcctacctgc taatagggtg gagcgcaaag ggagttgtgg gataacccat
      301 tgagcttgat taatgtgtgg cacccgcata cataacaatc gttcaatcaa gtctacctgc
      361 tttactattg acagtcaagc tttatcatta ctttcaggcg atgataggag gaccgcagcc
      421 ttgacagaca aagacaggac gtgcctgaat gatagtctaa cgcattcgca caacggttgc
      481 ctacaccctt cgcacaaccc gctatcgcga gaaggagcat gagcgcatat gtctagcaaa
      541 cgtctctccg ttattccaag aggggtctat ccgcccctac cgaccttttt tgatgagcag
      601 gaggagctgg acctggctac cttccaacgg cacatcgcca acctggcgca cactggcatc
      661 gcgggctgcg ttgtaatggg gtcaaatggc gaggcggttc acctgaccgc cgatgagcga
      721 gtacaggtaa ttgaggcggc gcgtgagatg gtcgctggcg agacgccgct gatcgccgga
      781 tgcggcgaac aatccacgcg ctcgaccatc gccaactgcc gcagggctgc gcaggccggg
      841 gccgatgccg cgctgatcct gccgccggtc tattacaaaa gccagatgaa ccggcgcgcg
      901 ctggtggctc attatcaggc cctcgccgat agcagcccgc tgcccatcgt catctacaac
      961 atgcctgcca acgccgccgg ccttgacctg gacgccgata ccgtctgtat cctggcggaa
     1021 catcccaata tcatctgtat caaggatagc gccggcgata tggtcaaact ggcgcagatc
     1081 gtggcgtgcg tccccgccac atttcacgtc ttcgcgggca gcgcgggcta cttccttccc
     1141 gccctgagcg tcggagtggt gggcgtcgtc gcggcgctgg ccaacatctt cccccgccag
     1201 gtgtgtcgcg tccaggaact gtttgacgcc ggtcgtatgg agcaggcgcg cgacctgcag
     1261 gccgccctgg tcgccgccaa ttcagccgtt acctcaatct acggtgtccc tggcctgaaa
     1321 gctgcgcttg agttgaccgc cggtcaggag ggacacctgc ctgcccactg cggcatcctg
     1381 cgctcccctt tgcaaccact caccgaacta gaacgcgcca ggctgcaaga gatcatcaag
     1441 ggagtaccgc gcattgaaga atgggacatc attaagagcg aatagctacc gcgcaatgcc
     1501 ccgccgcatc gacagtgttc ccagcacaag cgccgccagc gtgaatgccg ccagcaccgt
     1561 gatatccagc cccaactggt cggaggcgct gtaaaagata gcctgttcca gggcgtggcg
     1621 gccaaaggtc agcggcacga aagcggcgat ggtttgcagc cagcccggct cgaaggccag
     1681 cacgccgatg cccccggcca ggaagaacag gtagagcgcg acattgatcg agacggcgat
     1741 cacgacctgg atgcgccgca gcgcagcgcc gatggccaca cccacacccg cgctaaagag
     1801 cgagacgagc gcgataatca gcagcgcatt gacccagtag atgccttcag gttgcgtcca
     1861 gccgagcagg tcgcccagca gcagcaccag cgtccccagc acgaaggtga tggcgaagcc
     1921 ggccagcact ttgccggcga taatcgcacc gcgcgctgta ggcgacagca gcaattcctt
     1981 gaccgtattg cgttcccact cgcgcgcggt cgatagcccg ccgttgatga ggccgctgat
     2041 ggtgagcagc agcagaatcg tcggcaacac atcgtactga aagagttgca cgtcacggct
     2101 gcgcaggttg gtttcatgca tcgtcaccag caccgggctg gagctgccct gcgcctggta
     2161 atactgcgtg atggcatcgg ggacggagcg gcgaatatcg ttggtgaaat ccaggttcag
     2221 gttattgacg gtcacattga taggcgcgct gaggtgcgca tcaacacgct gcgtgaaatc
     2281 ggcggggatc gtaatgatcg ccaccacatc gatgttgttg agcagcacct gcgcctgcgt
     2341 gggggtcgcg tccgtcagcc ggaaaacatc cgcgttatgg atgatctgcg ccatctgctg
     2401 gcccttcgcg ccagagtcga gcgtgaccag cgccaccggg ctgcgcccga cggcggcaga
     2461 ccccagcgcg gccacgagca agaagcccag cggcggaaga agcgtcacag cgatattaac
     2521 cggctggcgc agccagacag cgatatcttt gctcataata atccaggcgc tgcgtaatgc
     2581 agtaaacata ttccctcctc ctttcgtgcc tcctgagcgc agctagaacg cagttcgttg
     2641 gaggcacggg tttcaagtct actcctgcgc tcttgctaat gcgccagtgt gctgcggcgc
     2701 agcacaaacg ccgcgatgag caccagcccc agggcatacg cgcccaggat caagacattc
     2761 gcggcgaggc cataagtgtt caaaatgaaa ttgtgaaagg catgctgctg caagacgatg
     2821 gcatagtaaa tggggaatat ctgggcgacc acctggatgg cgggcgtcgt aaacgaaatc
     2881 gggccaaacg cgccgctcag catgaaaagc gggatagaga cgccaaagga cagcgcaatc
     2941 accggctggc gatttttaat caatgtccca agcaccgcgc ccagcgcgat aaagagcagc
     3001 atggccagca gggtaaagcc aacgacttcg ccccaatggt cgggccgcac accgatgatg
     3061 acgatcaaca cgagcagcac aacgatcacc gccgagatgc tcatgacaaa tgcgcccagc
     3121 atcttgccga cgatcaccgc ccagcgactc gctggtgaga gcagcagctc tttaatcgtc
     3181 tcgttctcgt actcgcgcgc cgtggacgtg ccggattgca gcagaccgcc cagcatcagg
     3241 ccgatcacca ggatcgagac ggtcaggtag gggatgtaat ccgtgtcctg cggctgcaaa
     3301 tcgctttcac ttggcgtaat attcaccagg ctgggaaagg ctttcgcgta aaacgaggtg
     3361 atcgacagcg gaatggcgcg ccggatatcg ttggtgaaat cggtattcag gttgttgatc
     3421 tgcacgccca cctgcaccgg ctggttctgc cggatacgcg catcgaaatc ggcggggatg
     3481 gtgacaactg ccacgatctt gccggtggta atcagattct gcgcatcgct cgcgctggcc
     3541 gtttgcaaga caaaggaatg cgcgtggctc atagcgttat agaattgctg cgcatagggg
     3601 ccgctatcgt tcatcacgac cgccgtgggc gcctggccgc ccgaaaccac aaacaggcta
     3661 agcaagatca gaatgttgac cggcacaaag atgctgatga tggtaaagac gcgttcagtc
     3721 agagcgctcg tgatatcctt tttcatacag gcccacacga cccgcagatc atatttcgtg
     3781 cgctggaaca tggtatgcct tcctctcaga atgatcccaa catccccacg ggtataatta
     3841 gtcgcgtaac gcttttccgg tcagcagcag gaagatttca tccaggtttg gttcgcggat
     3901 ggcgatatcg cgaatgtcgc tttcctgcgc caggatgttg acgatctggg ctacgttgcc
     3961 atcgctgccc gatgtcgtga tcttcaacct ggctccctcc tggtcgatgt gcgtcactcc
     4021 tggcagatgc ctgatggcct cgacagcttg ctgcgtaggc cgcgccgtct ccacctcgat
     4081 gatgctgccg ccataggtct gcttaaggtg atcgggtgta tcgacggcga tcagtttgcc
     4141 gtggtcgata atggccagcc gctcgcatag cgcctgcgcc tcctcaaggt agttggtggt
     4201 aatcaagacc gttttgccct ggtcacgcag cgccaggatg taatcccaga tggcgcgccg
     4261 cgcctgcaca tcaacgccca gcgtcggctc gtcgaggtag atgagatgtg gatcgtgcag
     4321 cagcgcgcgg gccagcgcca gcctgcgctt catgccgccg gaaaaggtgc caacgcgcga
     4381 atccttgcgg tccagcagtt cgacaagcgt caacagactc gtgatgcgct cctttttctc
     4441 tttgcgggga atgttgaaca ggttggcatg aaaatccatg ttcgcccacg ccgtcaattc
     4501 gtcgtacagc gccgtctcct gcggcaccga gccgagcata ccgcgtatct tcctggactc
     4561 gtgacgtaca tcatagccca tgacacgcac ctcgccgctc gtcgctatgc tgaggccgct
     4621 aatcatgttg atggtggtcg tcttgcccga accgtttggc cccagcagcc cgaaaatctc
     4681 gccctgccgc acgctgagcg acaggtgatc gacggcccgg aacgtgccga agtttttgca
     4741 caggtcttgc atctcgattg caggaacgcc atccttcttc cctgttccgg tcgatgcgcc
     4801 tgcctgggtt atctcttgcg tcatgcgttc tctcctcgct ccatggtcag atcatccttc
     4861 acgaaaattt ggaagcaaat cagcgccaac tttgggatcg ggttcaactg ctgataggtt
     4921 ggcgagaatc tgctggagtt ttgcaatcaa atcttgctgc tcgtcctggg taaatccctt
     4981 catcaatgtt cccgtaagtg ccgctgcaac atccgggagg tagcgcatga tatcactgcc
     5041 ctcgctggta agaaacacat agacctgccg cctgtccttg cgatcatgtc tgcgctccac
     5101 aagcccactc tgctccagcc gcgtcacgat gcctgtaatc gtggcagcat ccacccctcg
     5161 cttctggctg attgcgccga tggtcatgcc cctttgctca tccaacagcg acaatacgcc
     5221 aaactgtggg ggggtgacca catacggttt gtcgtattcc tggcagcacg ccttcaatgc
     5281 ctcatagaag gcatacacta cgctgcgctg cgcacgaaat agccagtacc ccagaaaatc
     5341 ctccgcttga aacgtcataa tccatcctcg ttgcgatcat atttagtgcg taaacaattt
     5401 gagtgctaat atttagtata ttaacaatat actgcgaaga aaggaagttg tcaagtg
//
LOCUS       DASZAK010001117         3677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_560653, whole genome shotgun sequence.
ACCESSION   DASZAK010001117 DASZAK010000000
VERSION     DASZAK010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560653"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VGT44_19840"
     CDS             <1..246
                     /locus_tag="VGT44_19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding protein"
                     /protein_id="HEV2583123.1"
                     /translation="KERGQKSLPMLASAAAPGPLDAHQPRDAFGEMRKKVAIKCDLCA
                     GYNNQACVQACPTGAAIRVQPNAFFGTTEDILQQRAR"
     gene            299..1183
                     /locus_tag="VGT44_19845"
     CDS             299..1183
                     /locus_tag="VGT44_19845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583124.1"
                     /translation="MALATKSPEIRPGQRKRIWNAPASKAWWMWLAASLLLYALLLVL
                     YLNAVNTQKFPGPFSDPLRSFGIVAFLLVLATASFSLRRRFARNLPGKAQDWLWMHIW
                     LGITAILVALLHENFGLILRNYCANASCLTNAYGGDSALIALIVLVLSGVIGRALDQW
                     QARGIAQDASTNGVGIVRAIEERMLELELTIERLSAGKSEAFQQYCLQALESPGSNPA
                     IPVVPPQEERDFQRARNTLGSYAIMGQSLQRQKRARLIFRGWRIIHVVLAILALLIIS
                     IHSIMELLTNVLHLVKPV"
     gene            1199..2557
                     /locus_tag="VGT44_19850"
     CDS             1199..2557
                     /locus_tag="VGT44_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583125.1"
                     /translation="MQCPHCHSLLDDETIFCGNCGRQIAPLQASGATVVAAEDDTLTA
                     RSNASYAPPRPAPVDQQRARPHSDDATFVSFQSKSTPPVSPGQIVTPPPADQRPPRAP
                     NRRVLLIVGVLVILLAGGVVFALAQLQKNGSGGNPGANASGQVLFFDGANGTGNTDAL
                     KISVNGLSAPPSGSHYDAWLVNTVAEQSVALGTLNSNSGTFSVNFAGNGSNGQPGKNL
                     LSEGNQLEITLEQGNVTLPTGQVVLTATFPPMAFVHIKHLLFAFPTTPGHVGLLVGLD
                     QQTNLLNAQALVLQNVAANHNGSAIQCAAQSIIDIAEGQNGSNFKPLPTTCGSANATG
                     DGFGILGTNGYASTAAQHASLAATRTDSTATIRQHASEVEIGTANITGWVTTVDQDAL
                     KLLANPNNTALVQEIVTLSDHAYKGVDANGNGQIDPIKGEEGAVTTYLRGQLMATLTL
                     VP"
     gene            complement(3086..3214)
                     /locus_tag="VGT44_19855"
     CDS             complement(3086..3214)
                     /locus_tag="VGT44_19855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583126.1"
                     /translation="MQDTQINKFEMVEGTLPGPGQILLESSDRAVATAWGVSIIKQ"
     gene            3415..>3677
                     /locus_tag="VGT44_19860"
     CDS             3415..>3677
                     /locus_tag="VGT44_19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEV2583127.1"
                     /translation="MNQKGPLPKTMFEKIWEAHIVREVPGESTILYIDRHLVHEMTSP
                     QAFAGLHMAHRDIHRPLATIAVLDHNVPTVQGRKMLDVVDKESL"
BASE COUNT          831 a         1038 c         1070 g          738 t
ORIGIN      
        1 aaggaacgcg ggcagaaatc gctgcccatg ctggcaagcg ccgccgcgcc cggcccgctc
       61 gatgcacacc agccgcgcga cgctttcggg gagatgcgca agaaggtggc gatcaaatgc
      121 gacctctgcg ccgggtacaa caaccaggcg tgcgtgcagg cgtgtccgac cggcgcggct
      181 atccgcgtac agccgaacgc tttctttggc accaccgaag atatcctgca acaacgagcg
      241 cgttaaatat attctagtga cgacacgtgc ggtcgccacg ccgggaagga tgaagccgat
      301 ggctctcgcg acaaaatcgc ctgaaatacg accaggacag cgcaagcgta tctggaacgc
      361 gcccgcctcg aaagcgtggt ggatgtggct ggcggcttcg ctgctgcttt atgcgctgct
      421 gcttgtgctt tacctgaatg ccgtcaacac acagaagttc cctggcccgt tcagtgaccc
      481 actgcgtagt ttcggtattg tggcgttcct gctggtgctg gcgactgcct cgttttcgct
      541 gcgccgccgc ttcgcgcgca acttgccggg caaggcgcag gactggctgt ggatgcacat
      601 atggctgggc atcacagcta ttctggtggc gctgctgcac gaaaacttcg ggctgatcct
      661 gcgaaactac tgcgcgaatg ccagttgcct gaccaatgcc tacggtgggg acagcgcgct
      721 gatcgccttg attgtgctgg tgctgagcgg tgtcatcggc cgcgcactgg atcaatggca
      781 ggcgcgcggc atcgcccagg acgccagcac caacggcgtc ggcattgtgc gggccatcga
      841 agaacgcatg ctggaactgg aattgaccat cgagcgtctc agcgcaggca aatcggaggc
      901 gttccagcaa tattgcttgc aggcgctgga aagccctgga agcaatccgg ccattcctgt
      961 agtccctccg caggaggagc gcgatttcca gcgtgcgcgc aataccctcg gttcttatgc
     1021 aatcatgggg caatcgctgc aaaggcaaaa acgcgcccga ctcatcttcc gcggttggcg
     1081 tataattcat gttgtattag caattctagc gctgcttatc attagtattc attcaatcat
     1141 ggaattgctc acgaatgttt tgcacctcgt caaaccagta taaaaaggga ggaacagtat
     1201 gcaatgccca cattgtcata gcctattaga cgatgagacc attttctgcg gcaactgtgg
     1261 caggcagatc gcgcctttgc aggccagcgg cgcaaccgtt gtcgctgccg aggatgatac
     1321 gctcaccgcg cggagtaatg cgtcgtatgc gcccccgcgc cccgcgccgg tcgatcaaca
     1381 gcgagccagg ccgcacagtg acgacgcgac gttcgtgtcg tttcagtcaa agtcaacacc
     1441 gccggtgtca ccgggacaga tcgtgacgcc accaccggca gatcagcgcc ctccgcgcgc
     1501 ccctaaccgg cgcgttctgc tgattgtggg tgtgctggtg atcctactcg cgggcggcgt
     1561 tgtattcgcg ctggcccagc tccagaagaa tgggtcgggc ggcaaccctg gcgcgaatgc
     1621 gagcggccag gtgctgttct tcgatggcgc gaatggcacc ggcaacaccg acgcgctcaa
     1681 gattagcgtg aatggcctga gcgcaccgcc ttccggctct cactacgacg catggctggt
     1741 gaatactgtc gcggagcagt cggtcgcgct ggggacgttg aacagtaaca gcggaacatt
     1801 ttcggtaaac tttgccggaa atgggagcaa tggccagccg ggaaaaaacc tgctcagcga
     1861 agggaaccag ttagagatca cgctggagca gggcaacgtg acgctgccaa cgggccaggt
     1921 tgttctgacc gctacatttc ccccaatggc ttttgtgcat attaagcacc tgctcttcgc
     1981 ttttcccacg actcctggcc acgttggcct gctggtcggc ctggaccaac agaccaatct
     2041 tttgaacgca caggcgctgg tgctacaaaa cgtggccgcc aatcataacg gttcagcgat
     2101 ccagtgcgcc gcgcagagca tcatcgatat cgccgaggga cagaatggct cgaacttcaa
     2161 gccgctccca accacgtgtg gttctgctaa cgcgacgggc gatggttttg gcattctcgg
     2221 cacgaacggt tacgcctcga cagcggcgca gcacgcctca cttgccgcca cacgcaccga
     2281 ctcgactgcg acgattcgcc aacacgccag cgaggttgaa attggcacgg ccaatattac
     2341 gggctgggtg acgaccgtcg accaggacgc gctgaaattg ctcgccaacc cgaacaatac
     2401 ggcgcttgtg caggaaatcg tcacgctctc cgatcacgcc tataaagggg tggatgccaa
     2461 cggcaatggc cagattgacc ccatcaaagg cgaagagggg gcggtgacca cctacctgcg
     2521 cgggcaattg atggcgacgc tgacgcttgt gccgtagagg tgacgtctgg tggggacgct
     2581 tgcgtcgccc gcgtagcctg gaggacggaa cgaatcgtga tgcaaccagg tacgcgggcg
     2641 acgcaagcgt ccccactccg caccacgtcc acccccgccc ctacgggcga ggtcggttga
     2701 ccattttttt gacagtttcc gttaaaaaaa atgattcata cgcaacctcg ctcagcgagg
     2761 atacaatgaa gagcgaaaga ccgaagactt gtgtagccat catttttgct tgtggcataa
     2821 attatagaca caatgactat tgtcgagata gggtgagaca tggtatactt cataggtctc
     2881 ctctgaaggt tgcaaacgaa agaggaaggc catcaagggg tgtaggcgac tgatatctgg
     2941 acgaaggaag ggccaggagc cgaaggactt gatgcagacc cacacagaca ggcgtagtcc
     3001 ttcacaagtc aaagtgggcc accgagaaag agtcgagcaa gacaaagtac aagtcttgac
     3061 tcggcttttt catgcttgcg cttttttact gcttgatgat actcacaccc catgctgtgg
     3121 cgactgcacg atcactggat tcgagcaaaa tctggccagg tcccggcagc gttccttcta
     3181 ccatctcgaa cttgttgatc tgagtatcct gcatgatcgt ctcccggttg ttcactttgc
     3241 caggaaggga gcgtcggagg ccacttcagc tgccccaaaa atgacagtac aaaagatagt
     3301 tatgccgttg ggaacaggtg aaaatgtgag aagatgtgag agcccaaaat gtgcgataat
     3361 gggagggagt acgctcaatg acgagtctca cacgaccctg gagggacgag aagaatgaac
     3421 caaaaaggcc cgctgccgaa gactatgttt gagaaaattt gggaagctca cattgtgcgt
     3481 gaggtgcctg gtgagtccac catcctttat attgatcgcc atcttgtgca tgaaatgacc
     3541 tcgcctcaag cgttcgcggg tctgcacatg gcacatcgcg atatccaccg gcctcttgcg
     3601 accatcgcgg tactcgatca taacgtgccg acagttcagg gacgcaagat gctggatgta
     3661 gtggacaagg aaagcct
//
LOCUS       DASZAK010001118         1659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_703924, whole genome shotgun sequence.
ACCESSION   DASZAK010001118 DASZAK010000000
VERSION     DASZAK010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_703924"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1297
                     /gene="ppk1"
                     /locus_tag="VGT44_19865"
     CDS             <1..1297
                     /gene="ppk1"
                     /locus_tag="VGT44_19865"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEV2583128.1"
                     /translation="VVNPAMPPRVRTLLLDELEIQAGDMTVIDGPLGLGDLMELYSLD
                     RPELKDPPFIPRIPAVLRNEQNLFAAIQQQDILVHHPYDSFNSVFEFTHAAAHDPQVL
                     AIKQTLYRVGSNSPVVNSLLDAVENGKQVAVLVELKARFDEENNIEWARALERAGVHV
                     VYGLIGLKTHAKVALVVRREEDGLHRYVHLGTGNYNASTARIYEDVGFFTCRPDIGAD
                     ASDLFNSLTGYSLQSTYRKLLVAPSGLRKGIVERIQREIRVQQETGNGRLIFKLNALV
                     DPEIIHYLYRASQAGVVIDLIIRGICCLRPGVESVSDNIRVISIVGRFLEHSRIFYFG
                     NGGNEEIFLGSADMMQRNLDGRVETLFPIEDPTLREVIRTQLLDRFMADTVNASMLQS
                     DGQYVPVRPKPGEESLIPTDNAALASSYAQPVGKVALIS"
     gene            complement(1330..>1659)
                     /locus_tag="VGT44_19870"
     CDS             complement(1330..>1659)
                     /locus_tag="VGT44_19870"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906047.1"
                     /note="catalyzes the formation of
                     (R)-2,3-dihydroxy-3-methylbutanoate from
                     (S)-2-hydroxy-2-methyl-3-oxobutanoate in valine and
                     isoleucine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV2583129.1"
                     /translation="MYQGGMSSMRYSISDTAEFGDYVSGPRIIDERVRNEMRKVLREI
                     QDGTFATRWILENQAGRPSFLAMRRSNAAHQIEQVGSELRSMMPWLNAPNKKPEIAET
                     KNEPVSM"
BASE COUNT          353 a          444 c          451 g          411 t
ORIGIN      
        1 cgttgtcaat cccgcaatgc cgccgcgtgt tcgcactctg ctgctggatg aactggagat
       61 acaagctggc gatatgacgg tgatagatgg cccactgggc ctcggcgacc tgatggaact
      121 ctatagcctg gatcgtccag agctaaaaga cccaccgttt ataccgcgca tacccgctgt
      181 tttgcgcaac gaacaaaacc tgtttgctgc cattcagcag caggatatcc tggtgcatca
      241 tccctatgac agcttcaact cggtctttga gtttacccat gccgccgcac acgacccaca
      301 ggtgctggcg attaagcaga cgctttaccg cgttggctca aattcgcccg tagtcaattc
      361 gctgcttgat gccgtggaga acggaaagca ggtcgcggtc ctcgtcgaac tgaaggcacg
      421 tttcgacgag gaaaacaaca ttgagtgggc gcgagctttg gagcgtgcgg gggtgcatgt
      481 cgtctatggg ctgattggtt tgaagacgca tgccaaggtg gctcttgttg tgcgcaggga
      541 agaggatggt ttgcaccgct atgtgcatct tggtacaggc aactacaacg cttcgaccgc
      601 acgcatctac gaagatgtcg gtttctttac ctgtcgcccc gatattggcg cggacgcctc
      661 tgacctcttt aactcactaa ccggctactc gctccagagt acctatcgca agttgctggt
      721 cgcacccagt ggcctgcgaa aaggtatcgt ggaacgcatt caacgcgaaa ttcgcgtgca
      781 gcaggagacg ggcaacggac gcctgatttt caagctaaac gcgctggttg atcctgagat
      841 tattcactat ctctaccgtg cttcgcaagc aggcgttgtc attgatctga ttattcgcgg
      901 catatgttgc ttacgacccg gtgtcgagag cgtcagcgat aatattcgcg taatcagcat
      961 cgtggggcgt ttcctggaac acagccgtat tttctacttt ggcaacggcg gcaatgaaga
     1021 gattttcctg ggcagcgccg atatgatgca gcgcaacctg gatggtcgcg tggagacgtt
     1081 gtttcccatc gaagacccca ccttgcgcga agtcatccgt acccagttgt tggaccgttt
     1141 tatggcggac accgtgaatg cctctatgct tcaatccgat ggacagtatg tgcctgtccg
     1201 cccaaagccg ggagaagaat ctcttattcc gaccgacaac gctgctctcg catctagcta
     1261 cgctcagccg gtcggaaagg tagccttgat ttcatgaccg ttttatgtaa gcaaccgtca
     1321 ggatcgcttt tacatagata cgggttcgtt tttggtctca gcaatttcgg gttttttgtt
     1381 tggagcgttc agccagggca tcattgagcg cagttcgctg ccaacctgct cgatctgatg
     1441 cgcggcattg ctccggcgca tggccaggaa gcttgggcga ccggcctggt tttcgagaat
     1501 ccagcgtgtg gcaaaggtgc catcttgaat ttcgcgtaat actttgcgca tctcattgcg
     1561 cactcgttca tcaatgatgc gcgggccgga gacgtaatct ccaaactcag cggtgtcgct
     1621 gatcgagtag cgcatagaac tcatgccacc ctgatacat
//
LOCUS       DASZAK010001119         3446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_419568, whole genome shotgun sequence.
ACCESSION   DASZAK010001119 DASZAK010000000
VERSION     DASZAK010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419568"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1662)
                     /locus_tag="VGT44_19875"
     CDS             complement(139..1662)
                     /locus_tag="VGT44_19875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV2583130.1"
                     /translation="MQLCPNCGTQLPDGARFCGKCGRISNIVPNANQANQLSGQDNGG
                     SIDAYPVKRHARQPYRYTPPADNDAQSQPFGQSNPSAQPNQPSPANASSWSPAPVSAY
                     NQNTSRVPDQSPSAWQPGTYSSSPAKAASYPNEQPFQSYNAQPPAQPLVLPPATPAPA
                     SGNAKGFFPFRRRSPNRPRRRRGCAIGCLGVLLLLVIFGIIVVPMTQKVLAFGSAIST
                     QAPLSSQTGYMNTSDRVNILIMGYGGAGHDGAYLTDSMMVMSLIPSTHHTTLISVPRD
                     LWVQNPANSGNYTKINAVYPVAAGNTNTDPVAGGNAAAAKVSQILGMQVNYWLTINFA
                     GFKDFIDAIGGIDVNVPDGFTTLYPANDDPSINPNWMTVVFSKGLQHMNGARAISYAR
                     AREVTVNLAEGTDFARSKRQQLIMQAALAKLKDWHNWSSLYNAMGALQKTIYTNLSLA
                     DLGEFALHMDLTNAHHLGLSNQNVLVDATSSDGQSILQPGSGGWPSIITYVKNGLYN"
     gene            complement(1993..2766)
                     /locus_tag="VGT44_19880"
     CDS             complement(1993..2766)
                     /locus_tag="VGT44_19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEV2583131.1"
                     /translation="MSGLFEELKGQLHQEKGTALATIVRGETNVGAKLLVYPNKTAHG
                     SLGNAALEALVIEDAERAIWNAEARTHTYSVESETGAQAFDVFIEGFPPPPMLIIVGA
                     GHIAIPLTVFAKTLNFRVVIVDARSAFATRERFPMADELIVAWPDEVLEQMDLYPSTY
                     VAVLTHDPKFDEPTLKVVLSREVGYVGAIGSRKTKEERDERLRKQGLTDEQLKRIHGP
                     IGLNIGAESPEEMALAIMAEIVATQHRKDPSTMRDWTKK"
     gene            complement(2837..3154)
                     /locus_tag="VGT44_19885"
     CDS             complement(2837..3154)
                     /locus_tag="VGT44_19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEV2583132.1"
                     /translation="MRDILPDIERWREHGERIAIATVVSMAGSAPRRPGAKLAVSESG
                     ELSGSVSGGCVEPAVFESAQEVIKSGKPQMLEFGISEEENFEKIGLACGGTIHVFVER
                     LDW"
BASE COUNT          725 a          923 c          996 g          802 t
ORIGIN      
        1 gagcatgggg ccggatgtaa tcaccggccc ggtctgatag atgccgccga tcacgcgagg
       61 gttgcgctgg atcatggtct tctccctgtt cgcttgccag gttttctctt agattaccac
      121 aatcttgcgg ggctgtcatt aattatacag cccgtttttc acataggtga tgatcgatgg
      181 ccagccgcca ctacctggtt gcagaataga ttggccatcg ctggaagtgg catcgaccag
      241 cacgttctgg ttggaaaggc ccaggtgatg cgcgttggtc aggtccatat gcagcgcaaa
      301 ctcgccgagg tcggccagcg agaggttggt gtaaatcgtc ttttgcagcg cgcccatggc
      361 attatacagg ctggaccagt tatgccagtc cttaagcttc gccagggctg cctgcataat
      421 caactgctgg cgctttgagc gagcaaaatc ggtgccttcc gcgaggttga ccgtcacttc
      481 gcgcgcgcgt gcatagctga tcgctcgcgc gccattcata tgctgcaagc ccttcgagaa
      541 taccaccgtc atccagttcg ggttaatgct gggatcatcg ttggcaggat ataaggtggt
      601 gaagccatcc ggcacgttga catcgatgcc gccgatggca tcgatgaaat ctttgaagcc
      661 ggcaaagttg atcgtcaacc agtagttgac ttgcatgccc agaatctgcg agaccttcgc
      721 cgccgccgca ttgccgccgg cgaccgggtc cgtgttggta ttcccggctg ccacaggata
      781 aacggcgttg atcttggtgt agttgcccga attagcggga ttctgcaccc ataggtcgcg
      841 cggcaccgag atcaacgtcg tatggtgggt cgaagggatc aggctcatga ccatcatcga
      901 gtcggtcagg taggccccgt cgtgacccgc gcccccatag cccatgatga ggatattgac
      961 acggtcgctt gtattcatat aaccagtctg cgatgagagc ggcgcctgtg tagagatggc
     1021 cgagccgaaa gccagcacct tctgcgtcat tggcacgacg atgatgccga aaatcacgag
     1081 gagtaacagg acgcccaggc aaccaatggc gcagcccctg cggcggcgcg gacgatttgg
     1141 cgaacgccgg cgaaatggga aaaatccttt ggcgtttccg cttgccggcg cgggcgttgc
     1201 gggcggcaaa accagtggct gcgcgggcgg ctgtgcatta taagactgaa acggctgttc
     1261 attgggatag ctggccgctt tcgccgggga tgaagaataa gtacccggct gccaggcgga
     1321 gggagattga tccggcacac ggctggtgtt ctggttatag gccgagaccg gcgcaggcga
     1381 ccatgacgac gcgttagcag gcgatggctg gttcggttgc gcagagggat tcgactggcc
     1441 aaacggctgc gactgtgcat cattgtcagc cgggggagta tagcgatatg gttggcgtgc
     1501 atggcgtttc accggataag cgtcaatgga gccgccatta tcctgcccgg aaagctgatt
     1561 ggcctggttg gcattcggta caatattgga gatacggcca catttgccgc agaagcgcgc
     1621 gccgtcggga agttgtgtgc cacaatttgg acagagctgc atatgcgata actccttcat
     1681 gattttctat agcttgtaag agatacataa aagtataagg tgcatcataa tagagcatta
     1741 tacactatct atccgccatt agcatgtgaa gatcccaatc aatctgttac gtaccaggat
     1801 cagtcatcaa ttgtcaccct gagcgcagcg aagggtcttg gtcgataggc cgcgagatgc
     1861 ttcgctgcgc tcagcatgac agcaccgttg gtggttgaca aacttcatca tcgcgtccct
     1921 tcaacaaggc ctgtttatca atcttgacat tatttaaaaa cgattctgcc cgccgatggt
     1981 ttcaagctaa tgttatttct tcgtccaatc ccgcatggtt gaggggtctt tgcggtgctg
     2041 tgtggcgacg atttcggcca tgatcgccag ggccatctct tcgggtgatt ccgcgccgat
     2101 gttcaggccg atggggccgt gaatgcgttt caactgctcg tcggtcaagc cttgtttgcg
     2161 caggcgttcg tcgcgctctt ccttcgtctt acggctgccg atggcgccga cgtaacccac
     2221 ttcgcgcgat agtaccactt tgagcgtggg ttcatcgaac ttgggatcat gggtcaggac
     2281 cgcgacgtag gttgagggat acaggtccat ttgctcaagt acttcatccg gccaggctac
     2341 gatcagttcg tcggccatcg ggaagcgctc gcgcgtggca aaggcagaac gcgcatcgac
     2401 gatgacgacg cgaaagttaa gggttttggc aaaaacggtc aggggaatgg cgatatggcc
     2461 ggcgcccacg atgatgagca taggaggggg cgggaatccc tcgatgaaga catcgaaggc
     2521 ttgcgcgccc gtctcgcttt cgacgctata ggtgtgcgtg cgcgcctccg cattccagat
     2581 agcccgttcc gcgtcctcga taacgagcgc ctccaacgcg gcattgccca gcgaaccatg
     2641 cgccgtcttg ttggggtaga cgagcagttt cgcgcccacg ttggtctcgc cgcgcacaat
     2701 ggttgccagc gccgtgcctt tttcctggtg caactggcct ttcagttcct caaacagtcc
     2761 gctcatatgc tcttatccat ctaataacgc ccactgtagt tatctgtcat gcttcaatta
     2821 gtctgtaggg gccggtttac caatctaaac gttcgacgaa aacgtgtatg gtgccgccgc
     2881 aggccaggcc gattttctcg aagttctcct cttcgctgat gccgaattcc agcatctgcg
     2941 gcttgccaga tttaatgact tcctgggcgc tctcgaaaac cgccggttcg acgcagccgc
     3001 cgctgaccga gccggaaagc tcgccactct cactgactgc taattttgcg cctggtcggc
     3061 gaggcgctga acctgccatg ctgacgacgg tggcaatggc tatgcgttcg ccatgctcgc
     3121 gccagcgctc tatatcggga agaatgtctc gcatgttcta cctacctgcc tttcataagg
     3181 cataaaacta tagcatatcc aaccatacta tatcacatgg tgatggatgt gggtttctga
     3241 tcccccgaga tcccttgcgg tcgccatgcc ttgatagggc tgccgtgaag cgaaacacga
     3301 ctgtgacacg tgtacaacat gtggctctgg tagatgctca ttactggaaa gcgcttaaac
     3361 gatcactctt tatgaacata cagctagaca gtagagatga agggcatctg gggcagcaga
     3421 aaggatgacg cggttatgga aacatt
//
LOCUS       DASZAK010001120         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_63270, whole genome shotgun sequence.
ACCESSION   DASZAK010001120 DASZAK010000000
VERSION     DASZAK010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63270"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..963)
                     /locus_tag="VGT44_19890"
     CDS             complement(244..963)
                     /locus_tag="VGT44_19890"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV2583133.1"
                     /translation="MRVHQLIRTVRQSCKASNFVASVGVVALVGLLFVGHSEPQQTVA
                     GAAVHQPVPCPICATFKATTYNPLPMRLRPERYFAMRALARRAHVNSKQPARSATSST
                     GTTGVQPSKNTSTTTASPQGGSDINVFPYGTCTWWADQRYYQLHGDYVPWHSQANAWQ
                     WTARAYDFGWHVSSTPVLGAIINLQPGVDGAYSLGHVAIVEKILGNGHVIASNMAWGA
                     NPWVVTYVEFAPGPGVTFLYQ"
     gene            complement(1163..1384)
                     /locus_tag="VGT44_19895"
     CDS             complement(1163..1384)
                     /locus_tag="VGT44_19895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583134.1"
                     /translation="MDTDKQLETTPEQTKTLQKSTLPPLPKWPTSKRGPMVTEPPSGS
                     KRMHLRPEQVTETDYDQEHGKRETPKESY"
     gene            1568..2608
                     /locus_tag="VGT44_19900"
     CDS             1568..2608
                     /locus_tag="VGT44_19900"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV2583135.1"
                     /translation="MRVLITGATGLLGGHLIKELQQRGEEIRALVLPVENADKLKAQG
                     VEVARGDITDASTLGAAVNDVELVFHLAAMMGVWRPLADYRLVNVTGSENLYRAAQKA
                     GVRRYVHTSSHCVYGLGLGRFLTEKDPLRPDTHPYSVTKAEGDRLMRRLMLNSEVETV
                     ILRPGSFFGPGDKLNFGRMAQRMRDGKGAIIGRGDNHLPFCYITDIVQGFMLAAYHKQ
                     APGNVYNITNDRPLTQLEMFNAIADDVGGVRPTRHLPYAPIYYGSIVAEKVVAPVIRR
                     EPFVTTHGALMFGSDNKHSVEKARRELGFEPKVDLRVGIRLASAWFNEGGMAMPAVTD
                     ASRNAPLAGATR"
BASE COUNT          563 a          715 c          790 g          581 t
ORIGIN      
        1 atcttgtcag tggagttacc tattatatcc aggtctttgg agattatgca tcaacaccgc
       61 aagttgcttt catagcacaa taggcgcgcg cttgattgtc gttgcggggg aaccggtagc
      121 aggtttgctg aacgaatatg cgccagagat ccttcgctgc gctcaggatg acaagctccc
      181 cggcggtcat cctgagcgca gcgaaggatc tctggtggac ttatgagtaa tcaccagggg
      241 ttatcactgg taaaggaacg tgacgccggg gccgggagca aactcaacgt aggttactac
      301 ccagggattc gcgccccagg ccatattgct ggcgatgaca tggccgttgc ccaaaatctt
      361 ctcaacgatt gcaacatggc ctaaactata ggcgccgtct acgcccggct gcaaattgat
      421 gatcgcgccc agcaccggtg tgctggagac gtgccagccg aaatcatagg cgcgcgccgt
      481 ccactgccag gcattggcct gggaatgcca gggaacgtaa tcaccgtgca actgataata
      541 gcgttgatca gcccaccatg tgcaggtgcc atatgggaag acgtttatat ccgatcctcc
      601 ctgtggcgat gccgtcgtgg tcgatgtgtt cttcgacggc tggacgccgg tcgtacccgt
      661 actcgacgta gcggaccgtg ctggttgctt gctattgacg tgcgcgcgac gcgcgagcgc
      721 tcgcatggca aaatatcgct cgggtctcag gcgcattggg agtggattgt atgttgtcgc
      781 cttgaacgtg gcgcaaattg gacaggggac cggctgatgt accgccgcgc cagccacggt
      841 ctgctgcggc tcgctatgac caacaaataa cagaccaacc agcgcaacca cccccacact
      901 tgctacaaag tttgacgctt tacaggattg acgaactgtt cgaatgagct ggtgtactcg
      961 caatataccc ttcctttggt ctccaacttg cagcaccgca agttattgcc gagatacctc
     1021 aactccagca agacacgcgg ttacgaggac cggcgtatct cgtaaaaggg gaagtagtgg
     1081 caacgttgta gttataccat aggattaaag agatacagtt atatgaacag ttctatagac
     1141 atatcgggct aggagattgc ggctaatatg actctttggg tgtttcgcgt ttgccgtgtt
     1201 cctggtcgta gtccgtctcg gtgacctgtt ctgggcgcag gtgcatgcgt tttgagccgg
     1261 aaggtggctc cgtcaccatc gggccgcgtt tcgatgtcgg ccatttgggt agcggtggca
     1321 gtgtggactt ctgtaatgtc tttgtttgct ctggcgttgt ttctagctgt ttatccgtat
     1381 ccataaaata ccctccttac aggctgttgc tgcttgtatt ctatcacctg gacgcaatct
     1441 cctttagtac ttcagggccg ttcggctcta gtacctcgcg ctaactcgtt gcataattag
     1501 gggtaaagga cgtttgtcct ggtgtttact ctatctagag catacatgtc atatggaagg
     1561 ataattcatg cgagtattaa ttacaggcgc taccggcttg ctgggagggc atttgattaa
     1621 ggagctacag cagcgtggcg aggagatacg cgcgctggta ttgccggtgg agaatgctga
     1681 taaattgaag gcgcaaggag tggaagtggc gcgcggcgat attacggacg cgagtacgct
     1741 gggagcggcg gtcaatgatg tcgaactggt ctttcacctg gcggccatga tgggcgtctg
     1801 gcggccactg gccgattacc ggctggtcaa tgtcaccggc agcgaaaatc tgtaccgcgc
     1861 ggcgcagaag gccggtgtgc ggcgctatgt gcataccagc tcgcactgcg tctacggtct
     1921 tggcctgggg cgcttcctga ccgagaagga cccgctgcgc ccggatacgc atccgtacag
     1981 cgtcacgaag gccgaaggtg accgactgat gcggcggttg atgctcaaca gcgaggtgga
     2041 gacggtgatc ctgcgtccag gcagcttctt tggcccggga gacaagctga actttggccg
     2101 catggcgcag cgcatgaggg acggcaaagg cgccatcatc gggcggggag ataaccacct
     2161 gccgttctgc tatatcaccg atatcgtcca gggcttcatg ctggcggctt accacaagca
     2221 ggcccccggc aatgtctaca acatcaccaa cgaccggcct ctgacgcagc tagagatgtt
     2281 caacgccatt gccgatgatg tcggcggtgt gcgccccacg cggcacctgc cctacgcgcc
     2341 catctactac ggcagcatcg tcgcggaaaa ggtggtcgcg cccgtgatcc gtcgcgagcc
     2401 gtttgtcacg acgcacggcg cgctgatgtt cggcagcgac aacaaacaca gcgtcgaaaa
     2461 agcgcgccgc gagctgggct ttgagccaaa agtagacctg cgcgttggca tacggctcgc
     2521 ctccgcgtgg ttcaacgagg gcggcatggc catgccagcc gtgaccgacg ccagccgcaa
     2581 cgcccctctg gccggcgcca cgaggtaata gcatcacaga gtaagagaac aacagaagaa
     2641 gcgcggtcg
//
LOCUS       DASZAK010001121         2978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_456364, whole genome shotgun sequence.
ACCESSION   DASZAK010001121 DASZAK010000000
VERSION     DASZAK010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456364"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..245
                     /locus_tag="VGT44_19905"
     CDS             <1..245
                     /locus_tag="VGT44_19905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583136.1"
                     /translation="DVTSQVRYVAVTTYAVGSDRVIHFAVGDKSYSFSISSTTNLSSF
                     GGNPQTIQSGTSVIVTVQYNGNSATVLSISHSIHPI"
     gene            complement(328..1173)
                     /locus_tag="VGT44_19910"
     CDS             complement(328..1173)
                     /locus_tag="VGT44_19910"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEV2583137.1"
                     /translation="METFTDYYTVLGVDTGASSDTIKAAFKKLALQYHPDVYKGEDAE
                     ERMRLILLAYQTLSDPEARKHFDRQRAGHLGYAFDGAGASPRAAGSNSAAASAAKKKE
                     QNGRFAFPDLEGSGPVEFVLEGFTYTLWLDDALLLRQQGMLRGNAPHPRAAQGASSQI
                     LYACHRCQHRWNIAGKGNPPAACPSCHARDWADYLLLRCAHCHAVFESRELSDKLRGG
                     QLYNPYELFPLCPNCRRPQWCPAEDARVSILRAAEARRRAIMLIGVTVVALLVLGALA
                     LAFAR"
     gene            1394..2521
                     /locus_tag="VGT44_19915"
     CDS             1394..2521
                     /locus_tag="VGT44_19915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEV2583138.1"
                     /translation="MSEEINHTHHPEEHSDHDHEHNHSHDDHEREHAHDHSHDDHDHD
                     EQGGHSHGHGGHGHEHGRVDADLYGNKAGLRAVQISTLGMFLVAAIQFSIAGVGGSAG
                     LFADALHNLGDVFTTVALWIAFALSSRAANQRYTYGYYRSEDLAGIFIVLVIIASAVA
                     GAVESIQKLTSGNVPTQIYLSMAAALVGVIGNEVMAQYKISVGKRINSVPLVADGQHS
                     RIDGLTSLAAFVGLLFARFGLPIADPIAGIVITVVIVTVVYSTSRSVLQRLLDAVDPR
                     VVPSILTSASAVPGVEDVTDVRARWVGHTMHIAMNIEVDAELTLAKAHDIAEEVRHNL
                     FHSIQGVSEVVIHTDPSGASGDPHEALAHHLQEAQRPVVER"
     gene            complement(2565..>2978)
                     /locus_tag="VGT44_19920"
     CDS             complement(2565..>2978)
                     /locus_tag="VGT44_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2583139.1"
                     /translation="RANRHASQWAFAELHGLIAYKAVLSGSSLAIKVDADYTSQACPR
                     CGYTDRRNRPGNGLLFVCRNTQCPYHLRTGRPYRLHADLVGARNIAMRTLLTRQDWVG
                     TGVLSVRPDVSDRETKAARLARYAELRWSPDTSSL"
BASE COUNT          639 a          903 c          860 g          576 t
ORIGIN      
        1 gcgatgtcac cagccaggta agatacgttg ctgttacaac ctatgcagtt ggttcggaca
       61 gagtgattca tttcgcagtt ggcgacaaaa gctatagctt ttctatcagc tccactacta
      121 acctgagcag tttcggaggc aacccccaga caattcagag cgggacatca gtcatagtga
      181 cggtacaata caatggtaat agcgcaacag tactgagtat cagccactcg atccatccaa
      241 tctaatgctt ggatcagatc gatcagacta ctatatatcg gccaaattac atcactcgtc
      301 tcattgcgca cagcactgca taaaaattca ccgcgcaaac gccagcgcca gagcgcccag
      361 caccagcagg gcaaccaccg tcactcctat caacataatt gccctccggc gcgcttccgc
      421 ggcgcgcaga atggacacgc gcgcatcctc ggcgggacac cactgcggcc tgcggcagtt
      481 gggacagagc gggaagagtt cgtaggggtt atacagctgg ccgccgcgca gtttgtcgct
      541 caactcgcgg ctctcgaaga cagcatggca gtgcgcgcag cgcagcagca ggtaatcggc
      601 ccagtcgcgc gcatggcaac tggggcaggc cgcgggcgga ttgcccttcc cggcaatatt
      661 ccagcgatgc tggcagcgat ggcaggcata gagaatctgc gacgacgctc cttgcgcagc
      721 gcgcgggtgt ggcgcattgc cgcgcagcat accctgctgc ctcaggagca gggcatcatc
      781 cagccagagc gtataggtga aaccctccaa gacaaattcg acagggcctg acccctccag
      841 gtcggggaag gcaaaacgac cgttctgctc cttttttttc gcagcagaag cagccgcgct
      901 attgctcccg gcagcgcgcg gcgacgcgcc cgcgccatcg aaagcatacc ccagatgtcc
      961 ggcacgctgc cggtcgaagt gcttgcgcgc ctccgggtcg ctcaaggtct gataagccag
     1021 caagatgagg cgcatccgct cctcagcatc ttcgccttta tagacatccg ggtgatattg
     1081 cagcgccagc ttcttgaagg cagccttaat cgtatcgctg gacgcgcccg tatcaacgcc
     1141 taacacagtg tagtaatcgg taaatgtttc catcctataa ctgcaacgcc tatctcccct
     1201 ggttcgtgat aattcaactg gtcaacttat tatgcgctgt aatcgcagtt gttgacaatg
     1261 atttatctat ttgacgcttc agggtgagaa aagttgcaga actgctggat cgggacgaaa
     1321 tcacggctcg gtggtcagag actatgtgct atgctgtaaa tagcggaggc aacagagttt
     1381 gaaggaggca gtcatgagcg aagagatcaa ccatacgcat catcccgaag aacatagtga
     1441 ccatgaccac gagcataacc atagccatga cgaccatgaa cgcgaacatg cccacgatca
     1501 cagccatgac gaccatgacc atgatgagca gggcgggcat agccacggac acggcgggca
     1561 tggtcacgag catggccgcg tggatgctga cctgtacggc aacaaagccg ggctgcgtgc
     1621 ggtacaaatc tcgacgctgg gcatgtttct ggtagcggcc atccagttct ccatcgcggg
     1681 tgtcgggggc agcgcgggac tgtttgccga tgccttgcac aacctgggcg atgtcttcac
     1741 caccgtcgcg ctgtggatcg ccttcgcgct ctccagccgc gccgcgaacc agcgctacac
     1801 gtatggctat tatcgcagcg aagacctggc gggtatcttc attgtgctgg tcatcatcgc
     1861 cagcgcggta gccggagcgg tcgagtcgat tcagaagttg accagcggca acgttcccac
     1921 gcagatttac ctgagcatgg cggcggcgct ggtgggcgtg atcggcaacg aggtgatggc
     1981 gcagtataaa atctcggtcg gcaagcgcat caacagtgtg ccactggtgg ccgacggcca
     2041 gcactcgcgt attgacgggc tgacctcgct ggcggccttc gtcggactac tcttcgcgcg
     2101 tttcggactg cccatcgccg accccatcgc gggcatcgtc attaccgtcg tgatcgtcac
     2161 cgtcgtctac tccacctcgc gcagcgtcct acaacgcctg ctcgatgccg tcgatccgcg
     2221 tgtcgtcccc tcgatcctca catcggccag cgccgtgccg ggcgtcgagg atgtgaccga
     2281 tgtgcgcgcg cgctgggtag gacataccat gcacatcgcc atgaacatcg aggttgacgc
     2341 tgaattgacg ctggccaagg cgcacgacat cgcggaagag gtgcgccata acctcttcca
     2401 ctccatccag ggtgtttcag aggtagtcat ccataccgac cccagcggcg cgagcggcga
     2461 tccccacgag gcattggcac atcatctaca ggaggcgcag cgccctgtcg ttgagcgata
     2521 atcgctaccc cagcgccctg aacatatggc aagtcaatta ctccttacaa ggagcttgta
     2581 tccggactcc accgcaattc agcgtaccgg gcgaggcgcg ccgctttggt ctcacggtcc
     2641 gacacatcgg ggcgtactga caagacgccc gttcccaccc agtcctgccg ggtgagcagc
     2701 gttcgcatcg cgatgttgcg cgccccaacc aggtccgcat ggagtcggta ggggcgtccc
     2761 gtccgtaggt ggtacggaca ctgagtgttc cgacagacga acagcagccc gttgccgggg
     2821 cggttgcgtc tgtccgtata gccacagcgc gggcaggcct gactggtgta gtccgcatcg
     2881 accttgatgg ccagcgacga tccggacagg accgctttgt aggcaatcag gccgtgcagt
     2941 tcggcaaagg cccactgcga ggcgtggcga ttggcgcg
//
LOCUS       DASZAK010001122         3693 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_170667, whole genome shotgun sequence.
ACCESSION   DASZAK010001122 DASZAK010000000
VERSION     DASZAK010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170667"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..421
                     /locus_tag="VGT44_19925"
     CDS             <1..421
                     /locus_tag="VGT44_19925"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV2583140.1"
                     /translation="ANMRGYDKRDIVSDVLRFLSQSIDLALSAGVAWERIIIDPGIGF
                     GTTPQENLTLLRRLGELRALGRPILLGTSRKSTIGHVLGGLPPAERLEGTAATVALGI
                     AQGADVVRVHDVREMMRVVKMSDAVVHGSLEIEKEYS"
     gene            418..1836
                     /locus_tag="VGT44_19930"
     CDS             418..1836
                     /locus_tag="VGT44_19930"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HEV2583141.1"
                     /translation="MNYREALAYLYSLSDYERGGPFSRDPGEKLPREARLLERLDNPQ
                     RRYSSTLIAGTKGKGSTAAFIERVLRAAGLRTGLYTQPDLHTFRERMRVDGRLISEQE
                     VGELAPVVRAEVEQIEQQGELGSFNTYQVATALMFLYFARRHVQHAVLEVGLGGRLDA
                     TNIAQPLVSVIASISYDHMQILGNTLREIATEKAGIIKENGIVVTSAQSSEALLAIAE
                     VSRARHARLVRIGAASGDPAQAEVDAGRLPALIYRYALEERAAAHQRLTVYTPERVYR
                     GLEIPLAGQHQLENATLALAALELLREKGVTWDEEALRTGLREVQWPARIEVVGHDPM
                     VVVDGAHNADSMQKLLEALYSSFTLHRLIVVLSVARDKDLIGIAQVLQHVDIVVLTRV
                     ANPRAATIEELQAVFARHAPRVQVYSAGESSAAIHLALDLADAGDLVCATGSLYLAGE
                     ALRWIAARGNTDVAASIEGVDH"
     gene            complement(1830..2027)
                     /locus_tag="VGT44_19935"
     CDS             complement(1830..2027)
                     /locus_tag="VGT44_19935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HEV2583142.1"
                     /translation="MKLPMKSGRRRGLAAMSPERRREIASMGGRTSQARGTAHQWTAE
                     EASAAGKKGSARYMLKRIEHQ"
     gene            2237..>3693
                     /locus_tag="VGT44_19940"
     CDS             2237..>3693
                     /locus_tag="VGT44_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV2583143.1"
                     /translation="MQGLMMDYALTLQHAYNRAVRLFPMKEIVTQLDEGLHRYTYREW
                     GRRTAQLANALLKVGVKPGEPIATLAWNTYRHLELYFAVPCIGAVLHTLNMRLFADQL
                     VYIINNAEDKMIFVDGDLVPLLESFADKLPTVRQYVIMGDAPGATGKLQPSIDYETFI
                     AGQPEQVDWPSLDENAAAAMCYTSGTTGSPKGVVYSHRSIFLHSMATGMADSLALSEQ
                     EVILPIVPMFHANCWGLPHAGMMQGAKFVLPGRFMDPLRMARLMESERVTFAGGVPTI
                     WIGVLNALERETFDLSSVRMLICGGSAVPQSLIENLQRKGLTLVQAWGMTETSPLASV
                     SRIRSHQRDLPEEEQFRLRARQGTIVSGVEFRIVNVETGEQVPWDDTTFGELQVRGPW
                     IASAYYKDPATGEKFMDGWLRTGDVAVVNADGVIQLVDRTKDLVKSGGEWISSVELES
                     LIMGHFKVLEACVIGVPHPKWDERPLACVVAKPDFA"
BASE COUNT          688 a         1127 c         1137 g          741 t
ORIGIN      
        1 ggccaatatg cgcggctacg ataaacgcga catcgtcagc gatgtgctgc gcttcctctc
       61 gcagagcatc gacctcgccc tttcggcggg tgtagcgtgg gagcgcatca tcattgatcc
      121 cggtatcggc tttggcacaa cgccgcagga aaatttgacc ctgctgcgcc gcctgggcga
      181 gctgcgcgcg ttggggcggc caatcctgct tggcacatcg cgcaaatcga ccatcggcca
      241 cgtgctgggc ggcctgcctc ctgccgaacg cctggaagga actgccgcca ccgtcgcgct
      301 cggtatagcg cagggggccg atgttgtgcg tgttcacgac gtgcgtgaaa tgatgcgcgt
      361 ggtaaaaatg agcgacgccg tcgtgcatgg cagcctggaa atagaaaagg aatactcatg
      421 aattatcgag aggcgctggc gtacctctat agcctgagcg attatgaacg cggcgggccg
      481 ttttcgcgcg accccggcga gaagctgcct cgcgaggcgc gcctgctgga acggctggac
      541 aacccgcagc gccgctatag ctcgacgttg atcgcgggta cgaagggcaa aggctcgacc
      601 gccgctttta tcgagcgcgt gctgcgggca gctgggctgc gtaccggcct gtatacccag
      661 cccgacctgc ataccttccg cgagcgaatg cgcgtcgatg ggcggctcat cagcgagcaa
      721 gaggtggggg aactggcgcc ggtcgtgcgc gcggaggtcg agcagatcga acagcagggc
      781 gagcttggct cgttcaacac ctaccaggtc gcgaccgcgc tgatgttcct ctattttgcc
      841 cgtcgccacg tccagcacgc cgtactggag gtcgggttgg gtggccgcct ggatgccacc
      901 aacatcgcgc agccgcttgt ctcggtgatt gcctcgatca gctacgatca tatgcaaatc
      961 ctcggcaata cgctgcgtga gatcgccacc gagaaagccg gcatcattaa agaaaatggc
     1021 attgtcgtca cctcggcgca gtcgagcgag gcattactgg ctatcgcgga ggtgagccgc
     1081 gcgcgccacg cccgcctggt tcgcattgga gccgcgtcgg gcgaccctgc ccaggcggag
     1141 gttgatgccg ggcgcttgcc cgccctcatc tatcgctatg ccctggagga gcgcgccgcc
     1201 gctcatcaac gcctgaccgt ctacactcct gaacgggtct accggggcct ggaaataccc
     1261 ctggcaggcc agcaccagtt ggaaaatgcc acactcgcgc tggccgcgct ggagttgctg
     1321 cgagaaaagg gtgtcacctg ggacgaagag gcgttgagaa ccgggctgcg cgaagttcaa
     1381 tggccggcgc gcatcgaggt ggtcgggcat gatccgatgg tggtggtgga cggcgcgcac
     1441 aacgccgatt ccatgcagaa gttgttggag gcgctctaca gttcgtttac actgcaccgg
     1501 ctgattgtcg tgctgagcgt cgcgcgcgat aaagacctta taggcatagc acaggttcta
     1561 cagcatgtcg atatagttgt gctgacgcgc gtcgccaacc cgcgcgcggc cactatcgag
     1621 gagttacagg ccgtctttgc gcgtcatgcc ccgcgtgtac aggtctattc cgccggggag
     1681 agcagcgccg ccatccatct cgcgctcgac ctggcggatg cgggcgatct cgtctgtgct
     1741 accggctcgc tctacctggc cggggaagcc ctgcgctgga tagccgcgcg cggcaatacc
     1801 gatgtcgcgg cctcgatcga aggggtcgat cactgatgct ctatacgctt cagcatgtag
     1861 cgcgcgctac ccttcttccc ggcagccgag gcttcctccg cggtccactg atgcgctgtg
     1921 ccacgcgcct gcgatgtgcg cccgcccatg ctggcaatct ctcgtcggcg ttcgggactc
     1981 atcgccgcaa ggccgcgccg tcgcccactc ttcataggta gcttcatgct ccgctccttt
     2041 cgttttgtgc gtgtcgtatc acttcctaca gcataacagg aagtaacctg aacagcaaga
     2101 tgtttctggc tgtttgctag aaactaagca aattctaatg tggttgtgac cttgcgcttc
     2161 atctcgaatg gcatgtacaa tgaagcatct cgatcaagcc aattctttgc atcatttgca
     2221 taccgggagg aaactcatgc aaggcttaat gatggactat gctttgacgc tgcaacatgc
     2281 ctacaaccgc gccgtgcgcc tgtttcccat gaaagaaatc gtgacgcaat tggatgaagg
     2341 gctgcaccgt tacacctatc gcgagtgggg caggcgcacc gcgcagctcg ccaacgcgct
     2401 cctcaaggtc ggtgtgaaac caggagagcc gatcgccacg cttgcctgga atacctatcg
     2461 tcacctggaa ctgtatttcg ccgtcccttg catcggcgcg gtactgcata cactcaacat
     2521 gcgcctcttc gcggaccaac tggtctacat catcaataac gccgaggata agatgatctt
     2581 cgttgatggc gacctggtgc cgctgctgga gtccttcgcc gataaactgc cgacggtgcg
     2641 gcaatatgtg attatgggcg atgctcccgg cgcgacgggc aagttgcagc cgagcatcga
     2701 ctacgaaacc ttcatagctg gccagcctga gcaggttgac tggccctccc tggatgagaa
     2761 cgccgccgcc gccatgtgct acacctccgg cacgaccggc agcccgaaag gagtggtgta
     2821 tagccaccgc tcgattttcc tgcactcgat ggctaccggt atggcggatt ccctggcgct
     2881 ctccgaacag gaggtgatcc tgccgattgt gccgatgttc cacgccaatt gctggggtct
     2941 gccccacgcc ggcatgatgc agggcgcgaa attcgtcttg ccgggccgct ttatggaccc
     3001 tttgcgcatg gctcgcctga tggagtccga gcgcgttacc ttcgcgggcg gcgtgccgac
     3061 gatctggatc ggcgtcctga acgcgcttga acgcgagacg ttcgatctct cgtcggtgcg
     3121 gatgctgatc tgtggcggct ccgccgttcc gcaatcgctg atcgagaacc ttcaacgcaa
     3181 gggcctcacg ctggtgcagg cctggggcat gaccgaaacc tcgccgctgg cctcggtctc
     3241 gcgcatccgc agccaccagc gcgacctgcc ggaagaagag caatttcgcc tgcgcgccag
     3301 gcagggaacc atcgtatcgg gcgtggagtt ccgcatcgtc aatgtcgaga cgggcgagca
     3361 ggttccctgg gatgatacca ctttcggcga attgcaggta cgtggcccct ggatcgccag
     3421 cgcctactac aaagaccccg cgaccggcga gaagttcatg gatggctggt tgcgcaccgg
     3481 cgacgtggcc gttgtgaatg cagatggtgt gattcagctg gttgaccgca cgaaggacct
     3541 cgtgaaatct ggcggcgaat ggatttcttc cgtcgaactg gagagtctca tcatgggtca
     3601 tttcaaagtg ctggaagcct gcgtgattgg cgtgccccac ccgaagtggg acgagcgccc
     3661 actggcctgc gtcgtcgcca aacccgactt tgc
//
LOCUS       DASZAK010001123         4543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_133637, whole genome shotgun sequence.
ACCESSION   DASZAK010001123 DASZAK010000000
VERSION     DASZAK010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133637"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2925
                     /locus_tag="VGT44_19945"
     CDS             <1..2925
                     /locus_tag="VGT44_19945"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HEV2583144.1"
                     /translation="HTVSQVGQVRERGTAYIPQWRYVPATQLGRAESDVLVEEEVRKA
                     LIRLNPEINARPALADEVIYRLRAILLSVRSDGLVRANEEFTAWLRGERSMPFGSNNE
                     HTSVALIDFKHPKNNTYIMTTQLTYRNPEKRFDVVLLVNGIPLVVGETKTPTRPAITW
                     VDGADDIHNDYEVNVPAFFVPNVFSFATEGKTYRFGSIRMPLELWAPWRDVGEEQEAA
                     KAGKASEAGGMSGLAELQTATQSMLRPEVILDILQHFTLFATDKQHRKIKIICRYQQY
                     QAVNQIVERVVAGRIKKGLIWHFQGSGKSLLMVFAAQKLRQHPALKNPTVLIVVDRID
                     LDTQITATFNAADVPNMLAVDKRETLQTLLAQDTRKVLITTIHKFSEAPGVLNMRSNI
                     ILMVDEAHRTQEGDLGDKMRRALPNAFLFGLTGTPINKRDRNTFWAFGAVEDEQGYMS
                     RYSFEESIRDNATLPLHFEPRLVKLHVDKDAIDEAFANLTGHLSEDDQANLARMSARM
                     AALVKSSERVRAVVADIVQHFREHVEPNGFKAQIVTFDRQSCLQYKQELDKLLPKEAS
                     EIVMTVNASEKEYREYDRKKEEEEKLLDRFRNPRDPLKFLIVTSKLLTGFDAPILQAM
                     YLDKPMKEHSLLQAVCRTNRPYPNKSYGLIVDYLGVFDDVAAALDFDEQSMRRVITGL
                     EGLRAQLPGAVESCLQYFPAVDRAIVGYEGLLAAQECLPDNDRRDAYAEDYSVLARLW
                     EALSPDACLQPHMEDYRWLTQVYESVKPPGGHGKLLWHALGAKTTELINANIHVITTR
                     DDLDKLVMDAGALADILGLQEPKQVKEFELKIIARLRKHQDRPKFIELGRRLEALKER
                     HELGLITNLTFINQLLELARDVVQAEKEVEPVEERDEGKAALTELFNEARNGNTPVMV
                     ERIVNDIDEIVRIVRFPGWQETVAGDREVKQALRKTLFKYKLHQEQALFERAYEYIKQ
                     YY"
     gene            complement(2952..4406)
                     /locus_tag="VGT44_19950"
     CDS             complement(2952..4406)
                     /locus_tag="VGT44_19950"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEV2583145.1"
                     /translation="MKNNKVFKQKRFDTHHAIVIGGSMAGLLAARVLSDHFEQVTIIE
                     RDQLIEEAQPRKGVPQGRHVHALLAGGAAILGTYFPDLFASLARDGAVPVGTADIRRY
                     QLGVRVAAVPGPIKSLWQSRPFLEQHVRACLAARTNVQILDSCSVTRLRTEEERITGV
                     VLQRRGQQHEEDLAADLVVDASGRGSRAPQWLAALGYGQVEETSVTIDVGYASRIYRC
                     PDHPPTAWKVLIVLGPAPDYKRAGVIFPIEGGDWMVTLGGWLRDYPPDDDAGFLDYAR
                     GLSQPDLYEAIKQAEPLTPIALYKYSANRWRHYERLSRLPEGFIVIGDAVCAFSPVYG
                     QGMSVAAMEAKALDTCLREQQSRAGTLHSTSFPQRFHQAIAKNIKAAWMLSTGEDLRY
                     PETQGHRSLGTRLFTWYIRRVVGLTASRPQVAAAFFQVWHLLNPLRSLFEPRIFLAVL
                     GRELLFSRQKPGVVRPPDVPSSPGPVPPLEEAMR"
BASE COUNT         1011 a         1242 c         1382 g          908 t
ORIGIN      
        1 cacaccgttt cgcaggtagg acaggtgcga gagcggggaa cggcgtatat tccacagtgg
       61 cgctatgttc cggcgactca gcttgggcgg gcggagagtg atgtgctggt cgaggaggag
      121 gtacgcaagg cgctgattcg attgaacccg gagatcaacg cgcggcctgc gctggcggac
      181 gaggtgattt ataggctgcg ggcaattttg ctgtcggtgc gcagcgacgg actggtgcgg
      241 gccaacgagg agtttacggc gtggctgcgc ggcgaacgtt ccatgccttt tggttccaat
      301 aacgagcata cctccgttgc cttgatcgac ttcaagcatc ccaagaacaa tacctatatt
      361 atgacgacgc agttgaccta tcgcaatcca gagaaacgct ttgatgtggt actgctggtc
      421 aacggcatac cgctggtggt gggcgagacg aagacgccta cgcgaccggc gatcacgtgg
      481 gtcgatggcg cggacgacat tcacaacgat tacgaggtga atgttccggc ctttttcgta
      541 cccaacgtct tttctttcgc gaccgagggc aaaacgtacc gttttggctc gatccgtatg
      601 ccgctcgaac tgtgggcgcc ctggcgcgat gtcggggaag agcaagaggc ggcgaaggca
      661 ggcaaggcaa gcgaagcggg tggaatgagc gggctggcgg aactccagac ggccacacag
      721 agtatgctgc ggccagaggt gattctggat atcttgcagc acttcacgct ttttgccacc
      781 gataaacagc atcgcaagat caagatcatc tgccgctatc aacaatacca ggcggtcaac
      841 cagatcgtcg agcgcgtggt agccgggcgc atcaagaagg gactgatctg gcattttcag
      901 gggtcgggca agtcgctgtt gatggtgttt gccgcgcaga agctgcgcca gcatcccgcg
      961 ctcaagaatc ccactgtgct gatcgtggtt gatcgcatcg acctggatac gcagatcacc
     1021 gctaccttta acgcggcgga cgtgccgaat atgctggcgg tggacaaacg cgagacgctg
     1081 cagacgctgc tggcgcagga tacgcgcaag gtgttgatta ccaccattca caagtttagc
     1141 gaggctcccg gcgtgctgaa tatgcgcagc aatattatcc tgatggttga cgaggcgcat
     1201 cgcacccagg agggcgacct gggcgataag atgcgcaggg cgctgcccaa cgcttttctg
     1261 tttggcctga cgggtacgcc catcaacaag cgcgaccgca acacgttctg ggcctttgga
     1321 gcagtcgagg acgagcaggg ctatatgagc cgttattcct tcgaggaatc gattcgcgac
     1381 aatgccacgc tgccgctgca ttttgagcca cgcctggtaa aattgcacgt tgataaggac
     1441 gccatcgacg aagctttcgc caacctgacc ggccatctca gcgaagacga ccaggccaac
     1501 ctggcgagaa tgtcggcgag gatggccgcg ctggtcaagt cctcggagcg cgtgcgggcc
     1561 gttgttgctg atatcgtgca gcactttcgc gagcatgtcg agccgaatgg cttcaaggcg
     1621 caaatagtga cctttgatcg ccagtcctgc ctgcaatata aacaagagct ggataagctg
     1681 ctgccgaagg aagccagcga gatcgtgatg acggtcaatg cgagtgagaa agagtacagg
     1741 gagtacgacc ggaagaaaga ggaagaggag aaactgcttg atcgcttccg caaccccaga
     1801 gacccactca aattcctgat tgtcacctcg aaactgctga ctggttttga cgcgcccatt
     1861 ttgcaggcaa tgtacctcga caagccgatg aaagagcata gcctgctgca ggccgtctgc
     1921 cgcaccaatc gcccttatcc aaataagagc tatggtctga ttgtggacta cctgggcgtt
     1981 ttcgatgatg tcgcggcggc gctggacttc gatgaacagt cgatgaggcg cgttatcacc
     2041 ggcctggaag gactgagagc gcaactgccc ggcgcggttg aaagctgttt gcaatacttc
     2101 cccgccgtag accgcgctat tgtgggctat gaaggattgc ttgccgcgca agaatgcctg
     2161 ccggataacg acaggcgcga tgcctacgcg gaggattatt ctgtgctggc gcgcctgtgg
     2221 gaagcgctct cgcctgacgc atgtctccag ccacatatgg aggattaccg ctggttgacg
     2281 caggtctacg aatcggtcaa gccgccggga ggtcacggca aactgttgtg gcacgcgctg
     2341 ggagcgaaaa cgaccgaact gattaacgca aatattcacg tgattacgac gcgcgacgac
     2401 cttgataagc tggtcatgga tgccggggcg ctcgcggata tcctcggcct gcaagagcca
     2461 aagcaggtca aagagtttga actcaagatc atcgcgcgac tgcgcaagca ccaggatcgc
     2521 ccaaagttca tagaattggg gcggcggctg gaagctttga aagagcggca cgagctgggc
     2581 ttgatcacca atctgacctt tatcaatcaa ctgctcgaac tggcgcgcga cgttgtacag
     2641 gctgaaaaag aggttgaacc cgtcgaggaa cgggacgagg gcaaagcggc gctgaccgaa
     2701 ctgttcaacg aagcgcgcaa cggcaataca cctgtgatgg ttgagcgcat tgtcaatgat
     2761 atcgatgaaa tcgtgcgcat cgtgcgcttc cctggctggc aggagacggt cgcgggagac
     2821 cgcgaggtga agcaggcgct gcgcaagacg ctgttcaaat acaagctgca ccaggagcag
     2881 gcgctgtttg agcgcgcgta tgagtatatc aagcagtatt attaacagtg ctgcctgggt
     2941 gtatcattcc ttcacctcat tgcttcctcc agaggaggca ccggccctgg tgaactcggc
     3001 acgtccgggg gtctcactac gcctggcttc tgccgactga agagcaactc tctgcccagc
     3061 acagccaaga aaatacgtgg ctcgaacagc gaacgcaagg ggttgagcag gtgccacacc
     3121 tgaaagaaag ccgccgccac ctgggggcga gacgcggtca gaccaaccac gcggcggatg
     3181 taccaggtga aaagacgtgt gcctaacgat cggtgtccct gggtttctgg atagcgcaag
     3241 tcttctcctg ttgacagcat ccaggcagcc ttgatatttt tggcaatcgc ttgatggaag
     3301 cgctgtggga aactggtcga atggagggtt ccagcgcggc tctgttgttc gcgcaggcag
     3361 gtatccagcg cctttgcttc catggctgcc acggacatgc cctgcccata cacgggacta
     3421 aaggcgcaca cggcgtcacc tatgacgatg aagccttcag gcagacgcga caggcgttca
     3481 tagtgccgcc agcgattggc gctgtactta tacagagcga tgggagtgag cggttcggcc
     3541 tgcttgatgg cttcatagag gtctggctgg gacagaccgc gggcatagtc cagaaagccg
     3601 gcatcatcat cggggggata atcacgcagc catcctccca gcgtgaccat ccaatcgccg
     3661 ccctcaatcg ggaagatgac gcccgctcgt ttgtagtcgg gggctggtcc aaggacaatc
     3721 aagaccttcc aggcggtggg agggtgatcg gggcagcgat agatgcgaga ggcatagccc
     3781 acatcaatgg tgacgctggt ttcttccacc tgaccgtatc ctaaggcggc aagccactgg
     3841 ggcgcacgcg atccgcgccc gctggcatcg accaccaggt cggcggccag gtcttcctcg
     3901 tgctgttgcc cacggcgctg caagacgacg ccggtgatcc gctcctcctc cgtcctcaag
     3961 cgcgtgaccg aacagctatc caggatctgc acattggtcc tggctgcaag acaggctcgc
     4021 acatgctgtt ccagaaaggg tcggctttgc cacagcgact tgattgggcc tggaaccgct
     4081 gccacccgca caccaagctg atagcggcga atgtcagcgg tacccaccgg gacggctccg
     4141 tctcgggcga gtgaggcaaa gagatcgggg aagtacgttc ccaggatggc tgctccaccg
     4201 gcgagtaagg catgcacgtg ccgtccctgc gggacgcctt tgcgcggctg cgcctcttcg
     4261 atcaattgat cacgttcaat aatcgtaact tgttcgaaat ggtccgagag tacacgagcg
     4321 gccagcaacc cggccatact tccaccgatc acaattgcat gatgcgtatc gaatctcttc
     4381 tgcttgaata ctttattgtt tttcataaat gttctgcttc ctgatggtat tggcatttcg
     4441 attcatcggc ctaagagacc aaagcctttc aggagcgcgc atgacgctcg cgctccctcg
     4501 tgcttcttac aactaagata gccgagcaga gcggtgggcg tca
//
LOCUS       DASZAK010001124         1600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_312331, whole genome shotgun sequence.
ACCESSION   DASZAK010001124 DASZAK010000000
VERSION     DASZAK010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312331"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /gene="dnaX"
                     /locus_tag="VGT44_19955"
     CDS             complement(<1..1120)
                     /gene="dnaX"
                     /locus_tag="VGT44_19955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV2583146.1"
                     /translation="MASQSLYRKWRSQKFGELIGQEPIIRTLKNALSSGKPAHAYLFT
                     GPRGTGKTSTARLLAKTINCANPHDGEPCNECQQCREITAGNSFNVIEIDAASNRGID
                     SIRDLREKVMMPPSTGKYKVYVLDEAHMLTTEAFNALLKTLEEPPGYAIFVLATTDVH
                     KMLPTVLSRCQRFDFKRITTRQIVEHLNFVAEQEHITLERAAAELIARAAAGGMRDAL
                     SLLDQAIAYCGETISLAQVQTMLGVADPRAIYKLIMHVADLNSSGGLHLIHELAEAGA
                     DLRQIIAQVGEYWRALMLARAGADIAAILDLTEDETSEITQTARLFGLEELTECARIF
                     AQNDLVQKNQGTPQLGPELAFLACVELHRRPQVSQLPVV"
     gene            complement(1184..1406)
                     /gene="ffs"
                     /locus_tag="VGT44_19960"
     ncRNA           complement(1184..1406)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VGT44_19960"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT          340 a          452 c          458 g          350 t
ORIGIN      
        1 caacaactgg taattgactt acttgtgggc ggcgatgcag ctcaacacac gccaggaagg
       61 ccagctccgg gcctagctgc ggtgtcccct ggttcttttg caccaggtca ttctgcgcga
      121 agatacgggc acattcggtc aattcctcca gcccaaacag ccgggccgtt tgtgtaatct
      181 cgctggtctc gtcttcggtc aggtccagga tcgccgcaat gtcggcaccc gccctggcaa
      241 gcatgagcgc acgccagtac tctcccacct gggcgatgat ctggcgcaag tcagcgccag
      301 cttcggcgag ttcgtggatc aggtgcagcc caccagaact gttaaggtcg gctacatgca
      361 tgataagttt gtagatagcc cgtgggtcgg ccacgccgag catcgtctgc acctgagcca
      421 gggatattgt ttcaccgcag tacgcaatgg cctggtctaa cagactaagt gcatcgcgca
      481 tacccccggc ggcggctctg gcgatcagtt cagcggcggc tcgctccagt gtgatatgct
      541 cttgctcagc cacgaagttg aggtgctcca caatctgtcg cgtggtaatg cgtttgaagt
      601 cgaaacgctg gcaacgcgag aggacggtgg gcagcatctt atgcacatct gtggtcgcca
      661 ggacgaagat ggcgtagcct ggtggctctt ccaacgtttt gagcagggca ttgaatgctt
      721 ctgtcgtgag catgtgcgct tcgtcaagca catagacctt gtacttaccc gtcgaaggag
      781 gcatcatcac cttctcgcgc aggtcacgaa tgctatcgat accgcgatta gaggcagcgt
      841 caatctcgat cacgttgaac gagttgcctg cggtgatctc gcgacactgc tgacattcgt
      901 tgcatggctc accgtcgtga ggattggcgc aattgatggt tttggcaagt agacgggcag
      961 tgctggtctt gccggtacct cgcggtccgg tgaaaagata ggcgtgcgcc ggtttgccgc
     1021 tgctcagagc attcttgagc gtgcggataa tcggctcctg accaatgagt tcaccgaatt
     1081 tctgagagcg ccatttgcgg tagagcgact gtgaggccat attacaactc ccgctgccat
     1141 gcccaatgat agatcaccag ggttcaacca gaatcgaagc cgaaaacaca aagccgattt
     1201 tcgcccggtc ctgccgatca gcgtaaccag gccaccctac ggcacccaga gaggtcatcg
     1261 taccgttgct tcctcacaga cctagcgggg tttgaagaca tctgtcgcgc aggacccgat
     1321 tacgccgatc ggcaggaccg gaagaaaacc ggcccgttac cccacgataa atcgggggtc
     1381 tacagtagaa cagccgtatt gtactcctac agaggcgaaa tgtcaagaaa tgtacttccc
     1441 aatcagcagg ctatacttct ccttgacctc cgccggaatc ttgctacggt cggtctgaat
     1501 cgaagtcgcc agcacgctgg ggcagctgca ggtgttggcg ctacgatcag cggggatttt
     1561 cctggcgacg ctgcgcagaa tgcgttgggc attggcaata
//
LOCUS       DASZAK010001125         6636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_385124, whole genome shotgun sequence.
ACCESSION   DASZAK010001125 DASZAK010000000
VERSION     DASZAK010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385124"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /locus_tag="VGT44_19965"
     CDS             complement(<1..1606)
                     /locus_tag="VGT44_19965"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha/beta"
                     /protein_id="HEV2583147.1"
                     /translation="MRWFDPLNSALLADLQQLYNRLISKTIEVECSGASRLLPQTQIC
                     PECKTELGAGNRRYSRYAVCDTCGHHLVWSAFKRVEHLADPGTFKPIGRPLQPVDMFD
                     FADEHPYSARLIENQKKTGLRDAILVGRCRIGQIKAILGVLDFRFMGGSMGSVVGEQI
                     TLAFEYARRHRLPVVIVVNSGGARMQEGIISLMQMPKTAAAIQRFHAAGLLYISILAN
                     PTTGGVFASFASLGDVVLAEPKALIGFAGPRVAEQAVGQKLPAGSHQAESMLASGLLD
                     AIVEREDLPHVVAALLKPTVIQARTRHSHHLPTDQLQSFTALSPATTAWETVNIARHP
                     DRPTARDYIHSLSPNFLELQGDRCYGDDPTIISGLGDIDGRAVIFIGQQRLYGSRDDR
                     PAETQARPKPEGYRKAIRMMHLAAQLHCPLVTFVDTIGADPGVESEKHGMAWSLAHCL
                     STMSSLPVPIITAIIGEGASGGAVALAVADRIMMLQHAIYEIISPEGAATILYRDVRR
                     AEEVAARLKLTAADCLQLGVVDVIVPE"
     gene            1802..4060
                     /locus_tag="VGT44_19970"
     CDS             1802..4060
                     /locus_tag="VGT44_19970"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583148.1"
                     /translation="MRHLLRQKIGLQLFSLYLLFIIPVLLGGAGLYVYQQNVLTQNAF
                     QADLALSQAVALEVGANLRAASEIDSELSTAQAAKDLNLKQLTSVFANAAGAHPDISL
                     FFVCNLSGKMIINYPISPSSIGQNLGTSDYFQTALKSDTPYISSGSISTISDSYVVSI
                     AARITNDRGQIVGVMVIDLSLDQLTSRLNTVHQRLSPDSKVGLWVIDHKGQTIATTKQ
                     IFAFLTLPEDYAGLIQAAHDALQGQAGNFITHGQTTDWLYSYVPIPEEGWAVVVQRPT
                     DITFAVLTNFQHGLIVALALLILGASFFWLMMHLRVISPLTRLAKAVSLIRPDQPDQV
                     MHIDLLAKDQNRQDEIGELVQVFSVMESQIRSHFQKSDATIQTQFYTLDAIMRSIDEG
                     VLLESPDGLIVYANHIFNRAVGIPPQELMGKHLHDSELEKRASAILDCSNSYHEVAEP
                     ALRGHGPHMTEFQIRGTYKTRGRFVPGRRDIRMRIFHVRDAKGVLLGRGRIFQDITQQ
                     NEVEKIKRNLLAIVSHELRTPLTAIKGFATSLLDDADGEVDTNWQHYSLNQIVTESNR
                     MADLVTNLLDMAQVEAGTLKLYPALNSLDSLLDDAQAGASIEPSHVHFNVPEEMPMLY
                     VDRRRIEVVLRNLLENAQRYGGPDVNVEISATCGQQQTGLTINIADTGPGIPTHLIER
                     VFDRFYQVESGHERSSNGVGLGLAICRGFIEAHGGRIWAENRTDGTRGAIFHLWFPPT
                     ALHSQARSVTQS"
     gene            4093..4785
                     /locus_tag="VGT44_19975"
     CDS             4093..4785
                     /locus_tag="VGT44_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583149.1"
                     /translation="MPQEKCVLVVDDEQNILTFIRHSLEKHAFKVTTASNGWEALTEF
                     EQKTFVLVILDIMMPRMDGLEVCRRIRQISTVPIIILTALGEEADKIAALNQGADDYL
                     TKPFGVGELLARVQAVLRRASWADTPRSVGTQRFGNIEIDFEQRLVWRDGERVRLTPT
                     EFSLLQELALHPGKVFTHEVLLRRVWGTEHSRDAEYLRVYIGRLRRKIESDSSKPSYL
                     LTEPGIGYYLVK"
     gene            complement(4848..5570)
                     /locus_tag="VGT44_19980"
     CDS             complement(4848..5570)
                     /locus_tag="VGT44_19980"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2583150.1"
                     /translation="MSNEAAALSVQGITAGYGGPPIIDKVSLTARRGEITAIVGPNGA
                     GKSTLLKAVAGLIRSSTGRVFVEGNEVSGLPAEKLIRRGVAYVPQVENVFPDLTIREN
                     LDMGGYVRKSGVRERIEQLFLLFPDLKVASHRRASTLSGGQRTMLALARGLMVDPAVL
                     ILDEPSAGLSPRFRGLIWERIEQIRSTNVAVLIVEQNTRETLRHAQWAYVLVLGQNRL
                     EGTGADLLQDDEVVDLYVGRLS"
     gene            complement(5567..>6636)
                     /locus_tag="VGT44_19985"
     CDS             complement(5567..>6636)
                     /locus_tag="VGT44_19985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583151.1"
                     /translation="GVVVMILLIQESTRFIPDIPVFSTYIPPLREIIIGAVLVLFLRF
                     RPQGILPERRYIDRTPPNRVDVVADESPPLPVPETVAVASVAQPPDQDRPGEPIIVPM
                     ALVVEGLYKHFGGVVAVDGCSFQAPKGQVTGLIGPNGAGKSTAIDLISGFKVPDAGIV
                     VFEGAALQGLPPHRISRLGLIRTFQTPREWPGLTVLENVFLAHWDPERETLWRGLAGL
                     KQAHRPTTKTELERAREIRAELGLDHLVNERAGNLSGGQKRLVEFARIRMAEPQLVIL
                     DEPMGGVNPVLGERMAVAIEGFITAGTSVIIVEHNLPFIERVAQHVIVMSQGSVIAAG
                     PFESLRSNQSVIDAYLGEVTV"
BASE COUNT         1526 a         1817 c         1919 g         1374 t
ORIGIN      
        1 gctcagggac gatgacatcg acaacaccca gttgcaggca gtccgcggcg gtcaatttca
       61 agcgcgcagc aacctcctcg gctcttcgca cgtctcgata caagatagtt gccgctcctt
      121 caggggaaat gatctcgtag atcgcgtgtt ggagcatcat gatacgatcc gcgaccgcca
      181 gcgcgactgc tccaccactg gcgccttcgc cgatgatcgc ggtgatgatt ggcacgggta
      241 agctgctcat cgttgatagg caatgcgcca gggaccaggc catgccgtgt ttctcactct
      301 caacgcctgg atcagcgcca atcgtatcaa caaatgtgac caggggacag tggagctggg
      361 cagctaagtg catcatgcgg atggctttgc gatagccctc cggcttcggt cttgcctgtg
      421 tctctgcggg acggtcatcg cgagagccat atagacgctg ctgcccaatg aagataacgg
      481 cgcgtccgtc gatgtctccc agtcccgaaa tgatagtagg gtcgtcacca tagcagcggt
      541 ccccctgcaa ctccaggaaa tttggcgata gggaatggat gtaatcgcgc gcggtggggc
      601 ggtctggatg gcgagcgatg ttgaccgttt cccaggcggt ggtggcaggc gacagcgcag
      661 tgaaagattg aagctggtcg gttggtagat ggtgggagtg gcgagtcctt gcctggatca
      721 cggtcggttt caaaagcgcc gcgacaacgt gcggaaggtc ctctcgttcg acgatggcat
      781 cgagcagtcc agatgcaagc atcgattcgg cttgatgtga gccagcagga agtttttgac
      841 ccacagcctg ttctgccacg cgcggccccg cgaaaccaat cagcgccttt ggttcagcca
      901 gcaccacgtc ccccaggctg gcgaagctgg caaaaacgcc gccggtggtg gggttggcca
      961 gaatggagat atagagcaag cccgccgcgt gaaagcgttg aatggctgcg gcagttttcg
     1021 gcatctgcat caggctgatg atgccctcct gcatacgcgc gccaccactg ttgaccacga
     1081 tcacgaccgg caggcgatgt cgcctggcat actcaaaggc aagggtaatt tgctctccaa
     1141 cgaccgaacc catgctcccg cccatgaaac gaaaatctaa tactcccagg atggccttta
     1201 tctggccgat gcgacacctg ccgaccagga tggcatcacg cagcccggtt ttcttctggt
     1261 tctcgatgag ccttgcagaa tagggatgtt cgtcggcgaa gtcaaacata tctaccggtt
     1321 ggagcggccg accgatgggc ttgaaggtgc caggatcagc gagatgttcg actcgtttga
     1381 atgccgacca gacaaggtga tggccacagg tatcacaaac ggcgtaacgg ctataccgcc
     1441 tgttccccgc gccgagttct gtcttacact cagggcaaat ctgggtctga gggagcaatc
     1501 tagatgcgcc ggagcattcc acctcgattg tctttgagat caggcggttg tatagctgtt
     1561 gcaggtcagc cagtaaagct gaattgagcg gatcgaacca tctcatacat ctctccttaa
     1621 aagctctgtc acaattatag gtgaggcgct gtaaacatac cgtaaacagg aagaaggaaa
     1681 cataactaat tgcataacag tttcgcgtaa gaatgacttt cacagaaaaa ataattgcca
     1741 agcagggagg cgtaaggcat actaagaggt agtactgttt ttcttttcag cgaggtatag
     1801 tatgcgccac ctgttacggc aaaagattgg gctgcaactg ttcagtctct acctgctctt
     1861 tatcattcca gtgctgctgg gcggcgccgg attgtacgtg taccaacaaa acgttctaac
     1921 acaaaacgct tttcaagccg acctggctct ctcacaggca gtagcactgg aagtcggagc
     1981 aaatctgcga gccgcgtcag agatagatag cgaattatcg acagcccagg cagctaagga
     2041 cctgaacttg aagcagctca catccgtctt cgccaatgcc gcgggcgcgc accctgatat
     2101 cagccttttc tttgtctgca atctctccgg taagatgatt atcaactatc ctatcagtcc
     2161 aagtagcatc ggacaaaatc tggggaccag cgattatttt caaacggcgc tcaaaagcga
     2221 taccccgtat atctcatctg gcagtatcag cactatttcc gattcttacg tcgtctcaat
     2281 cgcggcacgt atcaccaacg acagggggca aatagtcgga gtcatggtga ttgacctgtc
     2341 tctcgatcaa ctgacttctc gtttgaatac cgtccaccag cgcctatcac ctgatagcaa
     2401 ggtgggtctg tgggtaatcg accacaaggg tcagacaatc gcgacgacca aacagatatt
     2461 cgcctttctc acactccccg aagactatgc gggtctgatc caggccgctc atgatgcgct
     2521 tcagggtcag gctggcaatt ttatcacaca cggtcagacc actgattggc tctacagcta
     2581 cgtgcccatt ccggaggaag gttgggcggt cgtggtacag cgcccgaccg atattacctt
     2641 tgccgtactc acaaattttc agcatggact catcgttgcg ctggcgctac ttattctggg
     2701 ggcaagtttc ttctggttga tgatgcacct gcgtgtcatc tcccccctca ccaggcttgc
     2761 gaaggccgtc tcgctgattc gaccagatca gcctgaccag gtgatgcata tcgacttgct
     2821 tgcgaaagac caaaaccggc aggacgagat aggcgaactg gtgcaggttt tttcggtgat
     2881 ggaatcacaa atccgctccc attttcagaa gagcgatgcc acgattcaaa cgcagtttta
     2941 tacgctggat gccatcatgc gcagcatcga cgagggagtg ctgctggaaa gtcccgatgg
     3001 gttgatcgta tacgccaatc acatttttaa ccgggcggtc ggtattccac cgcaggaatt
     3061 gatgggcaaa cacctgcacg atagcgaact ggagaagcgg gcgtctgcta ttttggattg
     3121 ttccaatagc tatcacgagg tggccgagcc agcactgaga ggacacggac cgcacatgac
     3181 cgaatttcag atccgtggca cgtataaaac gcggggtcgg tttgtgcctg gccgccgcga
     3241 catccgcatg cgcatttttc atgttcggga cgcaaaaggc gtcctgctcg gacgcgggag
     3301 aatatttcag gatatcaccc agcagaacga ggtagagaag atcaagagga acctgttagc
     3361 catcgtctcg catgaactcc gcacaccgct cacggccatt aagggattcg ccacgagtct
     3421 gttagatgac gccgacggcg aagtggatac caactggcaa cattacagtt tgaaccagat
     3481 cgtaacggag agtaaccgca tggcagatct cgtcacaaac ctgctggaca tggcgcaggt
     3541 ggaggcaggc acactcaaac tctatccagc gctcaactcg ctcgactcat tacttgatga
     3601 cgcccaggcg ggcgcatcga tagaaccgag ccatgttcac ttcaatgtac cagaagagat
     3661 gccaatgctc tacgtagata gaaggcggat tgaggtcgtg ttgcgcaatc tcttagagaa
     3721 tgcgcaacga tatggcggtc ctgatgtcaa cgtcgagatt tcagccacgt gcggtcagca
     3781 gcaaacgggg cttacgatca atattgccga tacgggacct ggtattccaa cacacctcat
     3841 cgagcgtgtc ttcgatcgct tctaccaggt cgaaagcggc cacgaacgta gcagcaacgg
     3901 agttggtctg ggactggcta tctgccgggg gttcatcgaa gctcacgggg ggcgcatatg
     3961 ggctgagaat agaacagatg gaacgcgagg agcaatattt catttatggt ttcccccgac
     4021 cgcgctgcat agtcaggccc gatcagtgac gcaatcatga gggaggatac ccgttgagaa
     4081 aggagcgcga agatgcctca agaaaagtgt gtgctagttg tcgatgatga gcaaaatatc
     4141 ctgacattta tacgccactc gctggaaaaa cacgccttca aagtgactac tgcgagcaat
     4201 ggatgggaag cgctgacgga gtttgagcag aagacttttg tactcgtgat attagacatc
     4261 atgatgccaa gaatggatgg gctggaggtg tgccgtcgca tacggcaaat cagcacggtg
     4321 ccgatcatca ttctcaccgc tctgggtgaa gaggccgaca aaatagccgc gctgaatcag
     4381 ggcgcagacg actacctgac caagcccttc ggcgtgggcg aactgctggc gcgggtacag
     4441 gcggtgctgc ggcgcgccag ctgggctgat acgcctcgtt cagttgggac gcagcgtttc
     4501 gggaatatcg agattgattt cgagcagcgg ctcgtctggc gtgacggcga acgggtcaga
     4561 ctcactccaa ccgagttcag tctgctgcaa gagttagcgc tgcatccagg caaggtattt
     4621 acgcacgagg tgctgctccg gcgcgtatgg gggacagaac acagtcgaga cgccgaatat
     4681 ttacgagtct atatcgggcg gctgcggcgc aaaatagaga gcgactcatc gaaaccgagt
     4741 tacctcctga ccgaacccgg catcggctat tacctggtca agtaacggga aactatccct
     4801 acgcacatag cgattgtcct gtagggcaat caaagattga cagatactta cgaaaggcgt
     4861 ccgacgtaga ggtcaacgac ctcatcgtcc tggaggaggt cggcgccggt gccttccagg
     4921 cggttctgac ccagcacaag gacgtaggcc cattgtgcat ggcgaagggt ctcgcgggtg
     4981 ttttgttcga cgatgagaac agccacgttg gtagaacgga tctgctcgat gcgctcccag
     5041 atcagcccac ggaaacgggg cgacagaccg gcgctcggct cgtccaggat cagaacggca
     5101 ggatcgacca tcaggccgcg cgccagggcc agcatcgtgc gctggccgcc gctcaacgta
     5161 ctggcccgcc ggtgcgaagc cactttgaga tcggggaaaa gcaggaagag ctgctcaatc
     5221 ctctcgcgaa cgccggactt gcggacatac ccacccatat cgagattctc ccgaatggtg
     5281 aggtcgggaa agacgttctc tacctggggg acgtaggcaa cgccgcgccg gatgagcttc
     5341 tccgcgggca ggccgctgac ctcatttccc tctacgaata ctctacctgt tgaagaccgg
     5401 atcagtccgg ccaccgcctt gagcagcgtg gatttgccgg ctccgttcgg tcccacgatg
     5461 gcggtgattt caccccggcg cgcggtcagg ctgaccttgt caatgatggg cgggccgccg
     5521 taaccggcgg taataccctg gacgctgagc gccgctgctt cgttactcat accgtcacct
     5581 cgccgaggta ggcgtcaatc acgctctggt tcgagcggag agactcgaac ggcccggcag
     5641 cgatcaccga gccttgggac atcacgatta catgctgggc gacccgttcg atgaagggca
     5701 ggttgtgttc gacgatgatg acggaggttc cggcggtgat aaacccctcg attgccaccg
     5761 ccatgcgctc gccgagcacg gggttcacgc ctcccatcgg ctcatcgagg atgacaagct
     5821 gcggctcggc catacgaatg cgggcgaact cgaccaggcg tttctggccg ccgctcaggt
     5881 ttcccgcgcg ctcgttgacc aggtggtcga ggcccagttc cgcccggatt tcgcgtgccc
     5941 tttcgagttc tgttttagta gttgggcgat gagcctgttt cagcccggca aggccgcgcc
     6001 agagcgtctc gcgttccggg tcccagtgag ccaggaaaac gttctccagg acggttagac
     6061 ccggccattc tcgcggcgtc tggaaggtgc ggatcaggcc aagacgggag atacggtgtg
     6121 gaggcagacc ctggagcgcg gcgccttcga agacaacgat ccccgcatcg ggaaccttga
     6181 accccgaaat gagatcgatg gcggtgcttt tgcctgcgcc gtttgggccg attagcccgg
     6241 tgacctggcc tttgggggcc tggaaggaac agccatccac ggcgacgaca ccgccgaaat
     6301 gtttgtaaag tccttcaact accagcgcca tcggcacgat aattggctcg ccgggtcggt
     6361 cctggtcagg tggttgtgct accgacgcaa cggcaacagt ttctggaact gggagaggag
     6421 gagactcatc ggctactaca tcaacccgat tagggggagt gcggtcgata tagcggcgct
     6481 ccggtaaaat accctggggc cggaagcgca agaaaagcac aagtaccgcg ccgataatga
     6541 tctcacgcag tggaggaatg taggtcgaga ataccggaat atcggggatg aagcgcgtcg
     6601 actcctggat cagcaagatc atgaccacca cgccca
//
LOCUS       DASZAK010001126         1793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_597393, whole genome shotgun sequence.
ACCESSION   DASZAK010001126 DASZAK010000000
VERSION     DASZAK010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597393"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VGT44_19990"
     CDS             <1..1077
                     /locus_tag="VGT44_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEV2583152.1"
                     /translation="DAKRAVAFGAQGIGLCRTEHMFMEQERLPIVQKMILAETKEERQ
                     AALDQLLPFQRSDFKGIFEAMVNPQTGEGYPVVIRLIDPPLHEFLPSYEDLLVEVTRL
                     EVSGGHEGELAEKRKMLEAVGAMREMNPMLGLRGCRLGLLFPEINVMQTRAILEAASE
                     LAKQGKKLQPKIMIPLVGHVNELKEVRRQLEAVAKEITAEAGAPIEYKFGTMIELPRA
                     ALTADQIAPVADFFSFGTNDLTQTTFGISRDDAEGKFLLKYVDGLEEPGLKTRVKILA
                     TNPFQTLDRDGVGQLVRMAAEKGRKANPHLELGICGEHGGDPDSIEFCHMVGLDYVSC
                     SPFRVPVARIAAAQAALTGAEKDK"
     gene            complement(1149..>1793)
                     /locus_tag="VGT44_19995"
     CDS             complement(1149..>1793)
                     /locus_tag="VGT44_19995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583153.1"
                     /translation="TNANTAAPCLGQFLGYGSTCDFNGQDCHTFCTLQEPPGVASTTR
                     WDVFAVIKTLWDYTTLSGQVVTQNQPAETDNAGNEYLINLYITWDGSQWHVTTSVPGN
                     TTDFVTPGPDCVAASDYVNMAAGGIYNSISVPGNPYEQIYWSSQQAANPASGCLLTAY
                     LQSGGVANPQPVAHCLYRFGVLLALDSAAQKNWPQMPLANAFEQTIAQQIAAQK"
BASE COUNT          369 a          511 c          578 g          335 t
ORIGIN      
        1 gacgccaaac gcgcggtggc ctttggcgcg cagggcatcg gcctgtgccg caccgagcat
       61 atgtttatgg agcaggagcg cctgccgatt gtgcagaaga tgatcctggc tgagacgaaa
      121 gaggagcgcc aggccgcgct cgatcaacta ctgccattcc agcgcagcga cttcaagggc
      181 atcttcgagg cgatggtgaa cccgcagacg ggcgagggct acccggtagt gattcgcctg
      241 atcgacccac cgctgcacga attcctgccc tcttatgaag atctgctggt cgaggtgacg
      301 cgcctggaag tcagcggcgg tcacgaggga gagctggcgg agaagcgcaa gatgcttgag
      361 gcggtcgggg cgatgcgcga gatgaacccc atgctggggc tgcgcggctg ccgcctgggc
      421 ttgcttttcc cggagatcaa cgtcatgcag acgcgcgcca tactggaagc cgcgagcgag
      481 ctggcgaagc agggcaagaa gttgcagccc aagatcatga tcccgctggt cgggcatgtg
      541 aacgaactca aagaggtgcg ccgccagctt gaagcggtgg cgaaggagat caccgccgaa
      601 gctggcgcgc ccatcgagta caagttcggc acaatgatcg agctgccgcg cgcggccctg
      661 accgccgatc agatcgcccc ggtggccgat ttcttcagct ttggcacgaa cgacctgacg
      721 cagaccacct tcggtatcag tcgcgacgat gccgagggca agttcctgct caagtatgta
      781 gatggcctgg aggagccggg tttgaagacg cgcgtcaaga ttctggccac gaaccccttc
      841 cagacgctcg accgcgacgg cgttggccag ctcgtgcgca tggctgccga gaagggtcgc
      901 aaggccaacc cgcacctgga actgggcatc tgcggcgaac acggcggcga tcccgacagc
      961 atcgagttct gccacatggt cggcctcgac tacgtcagtt gctcgccctt ccgcgtgccg
     1021 gtcgcccgca tcgcggctgc gcaggccgcc ctgacgggag cagagaagga taagtaaaca
     1081 ccaatagagc attatggcat ggcgaccgca agggatcgcc atgccaggac ttgtaaaccg
     1141 ctcgtctgtt acttttgcgc ggcgatctgc tgcgcgatgg tttgttcaaa ggcgttggct
     1201 agcggcattt gaggccagtt tttctgcgcg gcgctatcca gcgccagcag gacgccgaaa
     1261 cggtagagac agtgcgcgac gggctgggga ttggcgacgc cacccgactg tagataggcg
     1321 gtgagcagac agcccgaggc gggattggca gcctgttgag aactccagta aatctgctcg
     1381 taggggttgc ctggcaccga aatggaattg tagatgccgc cagcggccat gttcacataa
     1441 tcactcgccg cgacgcaatc gggtccaggc gtcacaaagt cggtcgtgtt gccggggacg
     1501 ctggtcgtaa catgccactg actgccatcc caggtgatat aaaggttaat caggtattca
     1561 ttcccggcat tgtcggtttc ggcgggctgg ttctgcgtca ccacctgccc actgagggtc
     1621 gtataatccc acagtgtctt gatgactgcg aagacatccc agcgagtcgt tgatgcaact
     1681 ccaggtggtt cttgtaacgt gcagaaggta tgacaatcct gcccgttaaa gtcacaggtt
     1741 gagccgtatc ccagaaattg tccaagacat ggagcggctg tattggcgtt ggt
//
LOCUS       DASZAK010001127         1631 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_560921, whole genome shotgun sequence.
ACCESSION   DASZAK010001127 DASZAK010000000
VERSION     DASZAK010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1631
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560921"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..1555
                     /locus_tag="VGT44_20000"
     CDS             332..1555
                     /locus_tag="VGT44_20000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2583154.1"
                     /translation="MIDMKPIASGATLLDNDTELNPNLTQQYHEIVDRYLSKELDYNE
                     AECNTECAGKDEQDLEEDIATMDTAPLELDEMHDMQEELPQMPTTPAMETSSILALPV
                     MGGEEITPARRVSMRRRRSEDQEEHPVNTADAGESDAVMTYLREIGRVPMITHEREIE
                     LAQRIEAGDRDAMQQFILANLRLVVSIAKRYVGRGLTLLDLIQEGNIGLIRAVQRYDW
                     RRGHRFSTHATWWIRQAISRAVADKGRTIRLPVYVNTALNRIRRERQRLLQELGREPT
                     EEELAEATGLDPIRMVELQAAPGAPVSLELPVGEDEEQELGDVLADTESASPEDIATT
                     QTLKNEVQRVLESVLTPRERLVLQLRFGLGNSQAHPLEQVGRELGITRERVRQIEAGA
                     LAKLRQPPVLERLRG"
BASE COUNT          388 a          493 c          434 g          316 t
ORIGIN      
        1 cgtaactttt tttatttcca atcgtatata gagtcataaa gagtaacggg catctgacgc
       61 aatgctgacc aactgcgaag cattcgtcgc ttcatcagtt gatgaaaacg cacttttgcg
      121 ttataattag aaggagcgac ggaatatttt cggccattcc tgtgtttcta aaagtagaaa
      181 gacagaacca gtgcatcata gacttcctcc cactcggttt tcggacgacg tgtccctatc
      241 gcaggagcgc ttcccctgat atcaggaccg aaccgacgga agatactgtt atcttgctgc
      301 ttgagatatt actgttcatg gagggcgcga tatgatagat atgaagccaa tcgcatcagg
      361 cgctacgctt cttgacaacg atacagaact gaatcccaac ctgacccagc aataccacga
      421 gatcgtcgac aggtacctct caaaagaact cgattacaac gaggcagaat gcaacacgga
      481 atgcgcgggc aaggacgaac aggacctcga agaggacatc gcgactatgg ataccgcgcc
      541 tctcgaactg gacgaaatgc acgatatgca agaagagttg cctcagatgc cgactactcc
      601 tgctatggaa acctcctcga tccttgctct cccggtgatg ggcggtgaag aaatcactcc
      661 agcccgccgt gtatccatgc gtcgccggcg ctcagaagat caagaggagc atcctgtcaa
      721 tactgcggac gcgggcgagt ccgatgctgt catgacctac ctgcgcgaga ttggtcgcgt
      781 accgatgatt acccacgagc gggaaatcga acttgcgcag cgcattgagg cgggagaccg
      841 cgacgcgatg cagcaattca ttctcgcaaa ccttcgcctg gtcgtcagta tcgccaaacg
      901 ttacgttggc cgaggcctga cgctgctcga cctgatccag gaaggcaaca ttggtctgat
      961 ccgtgccgta cagcgctacg actggcggcg cgggcatcgc ttctccactc acgccacgtg
     1021 gtggattcgc caggccatca gccgcgccgt cgctgacaaa ggccgcacca tccgcctgcc
     1081 tgtctacgtc aacaccgccc tgaaccgcat tcgccgcgaa cgccagcgcc tccttcagga
     1141 actcgggcgc gaaccaaccg aggaagagtt ggccgaggca accggtctcg accccatccg
     1201 catggtcgag ttgcaagccg cccccggcgc gcccgtctca ctcgaactgc cggtcggcga
     1261 ggacgaggag caggaactgg gcgatgtgct ggccgacacc gaatcggcct cgcccgaaga
     1321 tatcgccacc acgcagacgc tcaaaaatga agtccagcgc gtcctcgaat ccgtcctgac
     1381 gccccgtgag cgccttgtat tgcaactgcg cttcggcctc ggcaacagcc aggcccaccc
     1441 gctcgaacag gtaggccgcg aactgggcat cacccgcgag cgcgtgcgcc agatcgaagc
     1501 cggcgcgctg gcaaaattgc gccagccacc tgtattagaa cggctacgag gataaattca
     1561 tcaaaggagg ggcgcaatga attgcgtccc tccttttgtc ctggaaatac ctttctcatc
     1621 cctgtgtagg c
//
LOCUS       DASZAK010001128         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_241202, whole genome shotgun sequence.
ACCESSION   DASZAK010001128 DASZAK010000000
VERSION     DASZAK010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241202"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..118
                     /locus_tag="VGT44_20005"
     CDS             8..118
                     /locus_tag="VGT44_20005"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2583155.1"
                     /translation="MDRRYPLEHIAEAHRYVDTGHKKGNVVITVAHTHTT"
     gene            220..840
                     /gene="lspA"
                     /locus_tag="VGT44_20010"
     CDS             220..840
                     /gene="lspA"
                     /locus_tag="VGT44_20010"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV2583156.1"
                     /translation="MTKRRARIYDGLALLLALLVVTADQWTKALVVANLQPPDAGPQV
                     SIIGQYLILYYIQNNGAAFSLFANSVVLIVLILAAIALVVYLYARMINNGSLPSKLVF
                     GMIIGGALGNLIDRARHGGYVVDFISFRIPQIGFYFAIFNIADAAISVGVVLLFLMLL
                     FSGLRRTPKVPSPISPTDSGAGNQNGIAGVDSGKAANDAGQRSMPR"
     gene            840..1817
                     /locus_tag="VGT44_20015"
     CDS             840..1817
                     /locus_tag="VGT44_20015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV2583157.1"
                     /translation="MSFQSQPSLTTGDLFSYSIDSEAAGQRLDRYLTQAMQEMSRTVV
                     QQLIVDGAVLVNDKTSKPGYVLRAGDAVQVTTPGNRQSQQAMRPLALDLDIIYEDNDI
                     LVVNKPAGLVVHPAPGHRDDTLVNALLAYLPQASSADSSGSLDAPARAGIIHRLDKDT
                     SGLLIVAKNARAQQALVEQMKRREIVKRYLALVDGVVSPDRGSIDAPIGRDPRNRQQM
                     AITAVGSRDARTHFQVVQRFARHTLLLLQLETGRTHQIRLHLKAIGHPVVADPVYGSG
                     NVLKHVPLHRQFLHASQLQFRHPITGQQLELEAPLPDDLRAVLAKKEKL"
     gene            2102..2998
                     /locus_tag="VGT44_20020"
     CDS             2102..2998
                     /locus_tag="VGT44_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583158.1"
                     /translation="MQVLESTDRIPNDGDMPPRSLPPLRPILQEKKAQAKSKQREKLT
                     EEQPTAIIPVAQRASKLRARQTEDDGVNQEVKQWKALKAGETEDGEKTQHRASKKADA
                     IAPAVTDEPTSTTTETIAPAGKKPVKLPRPKIRSARTGRAWYTHPFFYLAVGMIFMLA
                     LWAILTSVASWWSVKSDDIQFGRPRTFQIDAVVGHNDSPSNPSHFIAINLNGRIEIIE
                     FPGGDGSKARIYIGPQLYGTGEDLVPVTLSFIDVNGNHRPDMVLHFQNTQIVFVNENG
                     GFRPATAQELPIIQRYIQQHGG"
BASE COUNT          833 a         1027 c         1005 g          794 t
ORIGIN      
        1 ctcagtcatc gatcgacgct atcccctgga acacatcgcc gaggctcacc gctatgtcga
       61 caccggacac aaaaagggca atgtcgtcat cacggtagcc catacccaca caacctgagt
      121 gtcaatccca ctattctgct ggacagtaaa gagggcttct caatcgttgt cagataggat
      181 acaatatgcg cgtatcgtcg aggaaagaga gaatatatcg tgacgaagag acgcgcgcgt
      241 atctacgatg gactggcact gctgctggca ctgctggttg tgacggctga ccagtggacg
      301 aaagccctgg tggtggcaaa cctgcagccg cccgacgctg gcccgcaggt ttcaatcatc
      361 ggccagtatc tgatcttgta ctacattcaa aacaacgggg cggcatttag cctgtttgcc
      421 aatagcgttg tcctgatcgt gctcatcctt gccgccatcg cgctcgtggt ctacctgtac
      481 gcgcgcatga tcaataatgg ctcattgccc tccaagcttg tctttggtat gattatcggg
      541 ggcgcgctgg ggaatctgat cgacagggcg cggcatggcg gctatgttgt tgattttatc
      601 tcgttccgca tcccgcagat cggcttctat tttgcgatct tcaacatcgc ggacgccgcc
      661 atttccgtgg gcgttgttct tttgttcctg atgttgctct ttagcggtct gcgccgtact
      721 ccaaaagtcc catctcctat ctcgccaacg gattctggcg caggcaatca aaacggcatt
      781 gcaggcgttg atagtggaaa agccgctaac gacgccgggc agaggagtat gccgcgctga
      841 tgagttttca atcccaacca tcactaacaa cgggcgattt gttcagctac tctatcgact
      901 ctgaggccgc cgggcagcga ctcgaccgtt acctgacaca ggcaatgcag gagatgtcgc
      961 gcaccgttgt tcaacagtta atcgtcgatg gcgcagtgct ggtcaatgat aaaacgagca
     1021 agccgggcta cgtgctgcgc gctggggatg ctgtccaggt aacaacgccg ggcaaccggc
     1081 agagccagca agctatgcgg ccacttgcac tggatttaga cattatctac gaggacaacg
     1141 atatcctggt ggtcaataag cccgccggac tggtggtgca tcccgcgcca ggccatcgcg
     1201 acgatacgct ggtcaacgcg ctgctggcgt acctgcccca ggcgagcagc gcggactcgt
     1261 ccggctcgct cgacgcaccg gcgcgagcag gcatcatcca ccgcctggac aaagacacat
     1321 cgggcctgtt gattgtggca aagaatgctc gcgcacagca ggcgctggtg gagcagatga
     1381 agcggcgcga aatcgtcaaa cgctacctgg cgctcgtcga tggcgtggtt tcgcccgacc
     1441 ggggcagtat tgacgcgccg attggtcgcg atcccaggaa ccgccagcag atggctatta
     1501 ctgccgttgg cagcagagat gctcgcacac acttccaggt tgtgcagcgc ttcgcccgcc
     1561 ataccctgct gctcctgcaa ctcgaaaccg ggcgcaccca tcagattcgc ctgcacctga
     1621 aggctattgg gcaccccgta gttgctgatc ctgtgtacgg ttccggcaat gttttgaagc
     1681 atgtgccgct ccaccggcaa ttcctccacg cctcccagct ccagttccgg catccgataa
     1741 ccggccagca gctcgaatta gaagcgcctt tgccggatga tcttcgggca gtactggcta
     1801 aaaaagaaaa actgtgattg ctcagatttt tcctgtagtc ccctggcggc atttgcctcg
     1861 cctatggtat actatgtcca gcgatgaact tcctcaccca acatcatcaa cgacataaaa
     1921 gtcagggtcg atgcctgtcc ctgatctatt tcttccaaac acgattggta gagcgttttg
     1981 tctattggag gctctaaccg actcacattc gtagcaacct ggccagaaag gttcaccgtt
     2041 tgcctgctat ttctcgtgta tcaagtattg ttttgttcca ggccgaaagg agtaacctga
     2101 catgcaagtg ctagaatcca ccgatcgcat cccgaacgat ggtgacatgc cgcctcgcag
     2161 tttgccgccg cttcgaccca tcttgcagga gaagaaagcc caggcaaaga gtaagcagcg
     2221 cgagaagctg accgaggagc aacctaccgc tatcataccc gttgcgcaac gcgcgtcaaa
     2281 attgcgtgcc aggcagacgg aggatgacgg ggttaatcag gaggtgaaac agtggaaagc
     2341 gttgaaagct ggcgaaacgg aagatgggga gaagacacag catcgcgcat caaaaaaggc
     2401 tgatgctatt gctcctgctg tgactgatga gccaacgtca acgacgaccg agaccattgc
     2461 cccggctggc aaaaagcccg tgaaactgcc gcgcccaaag atacgctctg ccaggacggg
     2521 acgggcgtgg tatacccacc cctttttcta cctcgccgtc ggtatgatct tcatgctggc
     2581 gctgtgggct atactcacgt cggtggcaag ctggtggagt gtcaagtcgg acgatatcca
     2641 gtttggccgc cccaggacgt tccagatcga cgcggtagtg ggtcacaatg actctccgag
     2701 caatccaagc cattttatcg ccattaatct gaatggccgt attgaaatca tcgagtttcc
     2761 cggcggcgat gggtcgaagg cacgcatcta cattggcccg cagctctatg gcaccggcga
     2821 agacctggta ccggtcacgc tcagtttcat cgatgtcaac ggcaaccatc gaccggatat
     2881 ggtcttacat ttccagaaca cccagatcgt cttcgtcaac gaaaatggag gcttccgtcc
     2941 ggcaacggcg caggagctgc cgatcatcca gcgctatata caacagcacg gcggataagc
     3001 catccggcaa gcaaagggag cgcgccgcca gggcaagaat acctggcggc gcgctccctt
     3061 tgctgaggta aggctaggat ctctgcgaaa atcgatcaca aagcaatttg ttatattagg
     3121 catgtgagca tgacatctgt ctccggttgc acgagggcga tagctagaag tggcgtccaa
     3181 aaccgtggcc gcaggaagtg cagggttggg tgccgttgcc tctatagcct ccgcacccac
     3241 agaaccacca atctctacat tgtccccaac cattggtaat gagaggttgt gtcgtgctgg
     3301 caggatcaac gtgtcggacg aggggaaagt gtagacgctg ctgggtagtg tcaggctgtg
     3361 taacttcaat tgggggttca agaactgctg cgttggacat agcattcttc ctttctctcg
     3421 aaagagcagt aaacaaactg ttcttttcct ttcatacttg acctaaagcc gagatacgcg
     3481 gcaaatagga cagaggaaaa aacaactttg aggaccggat ccattgggga agcgccaggc
     3541 atgggcatca gctcatccga ccttcgcgtg tttttcgcag gctggtggcc tcacgctgct
     3601 tcgcagtcct gtgggcatga ggccactcct tctggtgcga aggctccccg aaagggatt
//
LOCUS       DASZAK010001129         3350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_241297, whole genome shotgun sequence.
ACCESSION   DASZAK010001129 DASZAK010000000
VERSION     DASZAK010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241297"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..1064)
                     /locus_tag="VGT44_20025"
     CDS             complement(114..1064)
                     /locus_tag="VGT44_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEV2583159.1"
                     /translation="MLVKRISVISMLFCLALLTSCAKPSPSITTTTPTRTIAFTPTAV
                     PALTPSQSVTADTTPTHYTSHVLLSGIGRPDDLTFDPQGRLLFSDFYNGTISRLNANG
                     SVTILISGLAGPEGMVLLSDGTMIIAEQRTNRILTLAPGATSLTVLRALPGKPAGPPC
                     KDGVDNIALDATTNTLIVPDSPTGSVYRMSLDGKQLTLLASGIARPVGAAVDAQGTIY
                     IADECGGAVVRITPDGKSTRIAGFGKPDDVALDQHGNLLVIDLLPSIHALIRVRQSTG
                     KHEILESKGFIEPQGLVVDGHENIYVSDDYANIIVEFKPV"
     gene            complement(1247..2083)
                     /locus_tag="VGT44_20030"
     CDS             complement(1247..2083)
                     /locus_tag="VGT44_20030"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HEV2583160.1"
                     /translation="MSASTIYNEIQTSFKRGERIAIATVVKTAGAAPCGVGSKMLVRA
                     DGATSGSFAGPKTDGKVTQEALQAIRDGHSYLTHIHLDADQGEAVGSCGATLEVFFEV
                     LRPEPRLIIAGAGYVAQALARLASALDFRIVVVDDRRDLADPIVFDDKVQLTFGDIPQ
                     TIHELEPDESSWIVIVTRGHHLDKDALQAALETNATYVGMIGSPSKVKRIFKELLKEG
                     ISRERLEQVHAPIGLDLGAETPDEIALSIAAEMVMIRKKASGASLKTIHHLLEEEVPV
                     ES"
     gene            complement(2333..2971)
                     /locus_tag="VGT44_20035"
     CDS             complement(2333..2971)
                     /locus_tag="VGT44_20035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583161.1"
                     /translation="MVRVLIADDHPVFRFGLRTLLKADPAMEVVGEATNGEEAIAQAA
                     SLHPDVILMDLNMPGMNGIEAIRRIQADHPQVHILALTMFDDDDSVFAAMRAGACGYL
                     LKGAEGRETLQAIHVVSTGEAIFSPAIATRLMQYFGAPRPPGPSQPFPDLTEREREVL
                     VLIAQGYTNQAIAEQLVISPKTVRNHISSIFSKLQVTSRLEAILRAKDAGMS"
     gene            complement(3013..>3350)
                     /locus_tag="VGT44_20040"
     CDS             complement(3013..>3350)
                     /locus_tag="VGT44_20040"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583162.1"
                     /translation="YRASGITFSIQTPEPLPPLPAAVEVAIFRIVQEALTNVVRHAHA
                     HTCVVRLVCREQVTIEIIDDGQGFPPAYRHGVGLTSMRERAEELGGRWLIEPAPAGGT
                     CGRAQIPYR"
BASE COUNT          740 a          908 c          941 g          761 t
ORIGIN      
        1 gatttcgtga cctgccgcga aagggatgag cggttcaagc gctaaaccga tggcaacaga
       61 tgatccatcg gggacgtatt gcgctatcgc ggcggccatg gcagtgagtt tgctcataca
      121 ggtttgaact ccacgatgat gttggcatag tcatccgaca cgtaaatatt ctcatggcca
      181 tcaacaacca ggccctgtgg ctcaatgaaa cccttgcttt ctagtatttc gtgtttgcct
      241 gttgactggc gcacgcgaat aagcgcgtgg atcgaaggca agagatcgat gacaagcagg
      301 ttgccatgct gatcgagtgc aacatcgtct ggcttgccga agccagctat gcgagtgctc
      361 ttcccatcgg gcgtaatgcg cacgacagcc cccccgcatt catcggcgat atagatggtt
      421 ccctgcgcgt caactgcggc gcccacaggt cgcgcaatgc cggaagcgag caaggtcaac
      481 tgtttcccgt ccaggctcat gcgatacaca ctgcccgtgg ggctatctgg cacaatcagc
      541 gtgtttgtgg tagcgtctaa agcaatgtta tccactccgt ctttacaggg cggcccagcc
      601 ggcttacccg gcagcgctcg cagaaccgtg agtgaggtcg ctcccggcgc cagggtcagg
      661 atacggttgg tacgctgctc tgcgataatc attgtaccat cagaaagtaa aaccatgccc
      721 tcgggtcctg ccaatccact gatgaggatt gtcactgaac cgttcgcgtt aagtcgactg
      781 atcgtgccat tatagaaatc gctgaacagc aaccgcccct gtgggtcgaa ggtcaggtca
      841 tccggtcgcc caatgcctga gagcaagacg tgagacgtgt agtgtgttgg cgtggtgtca
      901 gcagtgacgc tctgacttgg ggtcagagct ggaactgcag ttggagtgaa agcaatagtc
      961 cgggtgggag tagtagtcgt aatcgaagga gatggtttag cacaggatgt gagcaaagcg
     1021 aggcaaaaaa gcatagagat gacgctgata cgtttgacga gcataaaaac cttcctcata
     1081 aagagatcgc cgggtttgcg gtttcaattg gcacaaccag cgtgctttta ccaatgcaaa
     1141 aaaccctcga gctacagtat taacgtttta ggcatgctgg aagtttcggg agtaatatgg
     1201 ggaatcttgg cgcagtagta acagaaccca gcccaaatcg caacttttag ctctcgacag
     1261 gaacctcttc ttccaggaga tggtgtatcg ttttgagtga agcgccactg gccttcttgc
     1321 ggatcatgac catttcggca gcaatactca gggcgatttc atcgggggtc tctgctccca
     1381 ggtctaatcc gatgggggcg tgtacctgct ccagccgctc gcgcgaaatg ccctctttga
     1441 gcagctcctt gaagatgcgc ttgactttgc tgggactgcc gatcatgcct acataggtag
     1501 cgttggtttc tagcgctgct tgtagggcat ccttatcgag gtggtggccg cgagtgacaa
     1561 tcacgatcca gcttgactcg tcaggctcca gttcgtgaat agtctgcggg atatctccaa
     1621 atgtcagttg cactttatcg tcgaagacaa tcgggtcggc caggtcgcgg cgatcatcca
     1681 ctaccacgat acggaagtca agcgcggacg cgagccgtgc cagcgcctgt gccacgtagc
     1741 cagcgcctgc aatgatcaat cgaggctctg gacgcaggac ttcgaaaaaa acctccaggg
     1801 tagctccaca actacctact gcttcaccct gatcggcgtc caggtggata tgggtcaggt
     1861 aagaatgacc atcacgaatg gcctggagcg cttcttgcgt taccttccca tcggtttttg
     1921 gcccggcgaa actgccgctg gttgcgccat cggcgcgcac taacatctta ctgccaacac
     1981 cgcacggagc agcgccggca gttttgacga cggtggcgat ggcaatcctt tcaccacgct
     2041 taaaggacgt ttgaatctcg ttgtaaattg ttgaggcact cattggctgt tacccgttct
     2101 tttttcgttc ctggctatgc tcatacattg agtactatgt aatttatatc atacaacata
     2161 atgtggggta agtggtatgg cattccagtt tagaaacatc aattatgctc atggttaact
     2221 gatgtatttg gatcaagttc ggttggccta tcggtgtgct tctgacgaaa agagacgatg
     2281 taccatataa ctagcaacaa ggagatacac tgaaacaggc tttccttgct tgctagctca
     2341 tacctgcatc ttttgcacgc aggatggcct ccagacgact ggtcacttgc agcttgctga
     2401 agatcgagga gatgtgattg cgcaccgttt tggggctgat gaccagttgc tccgcaatcg
     2461 cctggttcgt gtagccttgc gcaatgagca caaggacctc tcgttcacgc tccgtcaggt
     2521 cagggaacgg ctgggaagga cctgggggac gtggtgctcc aaagtactgc atcaggcgcg
     2581 tggcgatagc cgggctaaaa atggcctctc cggtgctgac cacgtggatg gcctgcaacg
     2641 tctccctgcc ctcagcaccc ttgaggaggt agccacatgc cccggcacgc atggccgcaa
     2701 agaccgaatc gtcatcatca aacatggtca gcgccaggat atgcacctgg ggatgatcgg
     2761 cctggatgcg ccggattgcc tcgatgccat tcatccctgg catgttgaga tccatgagga
     2821 tcacatccgg gtgcagtgaa gcggcctggg cgatcgcctc ctccccattg gtcgcttctc
     2881 ccacgacctc catcgccggg tccgctttca acagggtgcg taacccaaag cggaagacgg
     2941 gatgatcatc ggcaatcaac acgcgaacca tacatctctc tcctttcgcc tcgatgccaa
     3001 acgctcccgc actcaccggt acgggatctg tgcgcgacca caggtaccgc cagccggtgc
     3061 gggttcgatc agccaccttc ctccgagttc ctcggcgcgt tcgcgcatgg aggtcaagcc
     3121 cacaccgtga cgataggcgg gaggaaagcc ctgcccgtcg tcgatgattt caatggtcac
     3181 ctgctctcga cacaccaggc gaacgacgca ggtatgggca tgggcatgac gcacgacgtt
     3241 ggtcagtgct tcctgcacga tgcggaagat cgccacctcg accgcggctg gcagtggagg
     3301 aagtggctct ggggtctgga tggagaaggt gatgccgctt gcccggtact
//
LOCUS       DASZAK010001130         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_204688, whole genome shotgun sequence.
ACCESSION   DASZAK010001130 DASZAK010000000
VERSION     DASZAK010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204688"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(482..937)
                     /locus_tag="VGT44_20045"
     CDS             complement(482..937)
                     /locus_tag="VGT44_20045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV2583163.1"
                     /translation="MATRTTMHVDVVTAERELYSGEAEELIAPGSEGEMGILPRHAAL
                     LATLKAGQMIIKLGGLEEPLFVSGGFIEVSNNQVTVLADSAEHAEEIDQARAEAARRR
                     AQELLEQSTSNTERADLTAALERAVSRLRVAELARRRPSRRIEMPRQEQ"
     gene            complement(949..2364)
                     /gene="atpD"
                     /locus_tag="VGT44_20050"
     CDS             complement(949..2364)
                     /gene="atpD"
                     /locus_tag="VGT44_20050"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV2583164.1"
                     /translation="MTTQSLTKGTVVQVLGAVVDVEFPQDHLPEIYNEVRVRLAGADH
                     DLSLEVEQHLGNNWVRCVAMGPTDGLQRGIEVIDTGQPITVPVGPKTLGRIFNVLGQA
                     VDNKPEVEDAPRRPIHRLAPTLEDQAATIEVFETGIKVVDLICPFMKGGKIGAFGGAG
                     VGKTVIIQELINNIAKGHGGYSVFAGVGERTREGNDLYHEMQEAKVIDRLAMVFGQMN
                     EPPGARLRVALSGLAIAEYFRDEEGKDVLLFIDNIFRFTQAGAEVSALLGRMPSAVGY
                     QPNLAEEMGELQERITSTKKGSVTSMQAVYVPADDYTDPAPATTFAHLDSTLVLERAI
                     FEQGIYPAIDPLTSTSRILDPNVVGREHYDIARAVQRVLQRYKDLQDIIAILGIDELS
                     DEDKLTVARARKLQRFFSQPFTVATVFTGLEGRYVPLKETVRGFKEILEGKYDHIGET
                     NFYMKGSIDEVVESYEKEQKG"
BASE COUNT          456 a          702 c          675 g          545 t
ORIGIN      
        1 tgggtattgt gtgtacatca agcggctcag cgatgtagag ctgccaatcc ttgagcaaat
       61 cgtacaacag tcctatgagt acatcaagtc aaagtcccaa gacgggcctc tagaccgaat
      121 agtatggcaa actgaaaaat aggcgtttct gcctccatgg aactcccgcc tcttcttttc
      181 tgagtaggtg ctcttgatac ggtctctcaa gtatttctct ccaacgggta atctcatctc
      241 tgatgtggtg tgaacttttg cccacttgct gcaagaagag gaagcgatct ttttattcgg
      301 cgagacatac ccctctgtct ggctgtagat cccaggatag aatgggagaa aattgagtga
      361 atgaagcggg cagttctctc tcgtgtcaga gagagtcgcc cgcttcatta tacagattcg
      421 gcctcactta attccgtgat ctacaggatg acaacttctt accaaaccgt gtccgcctct
      481 attactgctc ctgtcggggc atttcgatgc ggcgcgatgg gcggcggcgc gccagttcgg
      541 ccacgcgcag gcggctgacg gcgcgttcca gagctgccgt cagatcggcg cgctcggtat
      601 tcgaggtgga ctgctccagc agttcctggg cgcggcggcg cgccgcctcg gcgcgcgcct
      661 ggtcgatctc ctcggcgtgt tcggcggaat cggccagcac cgtcacctgg ttattggaaa
      721 cctcgatgaa gccgccggag acgaagagtg gctcttctaa gccgcccagc ttaataatca
      781 tctgaccggc tttgagcgtg gcgagcaggg cggcgtgtct gggcagtatt cccatctcgc
      841 cctctgagcc aggcgcgatc agctcttctg cctcgccact gtagagttcg cgctcggcag
      901 tcacaacatc gacatgcata gtggtgcgtg tcgccatgct ttcgcccctt agcctttctg
      961 ctctttctcg tagctctcca ccacctcgtc gatgcttccc ttcatgtaga agttggtctc
     1021 accgatgtgg tcgtacttgc cttccaggat ttccttgaag cctcgcacag tctctttgag
     1081 aggcacatag cggccctcca gtccggtgaa gacggtggcg acggtgaacg gctgcgagaa
     1141 gaaacgttgc aacttgcggg cgcgcgctac ggtaagttta tcttcgtcag aaagctcgtc
     1201 aatacccaga atagcgatga tgtcctgcaa atccttgtaa cgctggagca cgcgctgcac
     1261 ggcgcgcgca atatcataat gctcgcgacc caccacgttc gggtcaagaa tgcggcttgt
     1321 cgaggtcagc gggtcgatgg cgggatagat gccctgctcg aagatggcgc gttccagcac
     1381 aagcgtcgag tccaggtggg caaatgtcgt tgccggagcc gggtcggtat agtcgtcggc
     1441 tggcacatag acggcctgca tcgatgtgac cgaacccttc ttcgttgagg tgatgcgctc
     1501 ttgcagctcg cccatctctt ccgccaggtt gggctggtat cccaccgccg acggcatacg
     1561 accgagcagc gccgatactt ccgcgccggc ctgggtgaaa cggaagatgt tgtcgatgaa
     1621 gagcagcacg tccttgccct cttcgtcgcg gaaatattcg gcgatggcaa ggccgctcaa
     1681 ggccacgcgc aggcgcgcgc cgggcggctc gttcatctgg ccgaagacca tcgccaggcg
     1741 gtcgataact ttggcttcct gcatttcgtg gtaaaggtcg ttgccctcgc gggtgcgctc
     1801 gcccacaccg gcgaagacgc tatagccgcc gtgtcccttc gcgatgttgt tgatcagctc
     1861 ctggatgata acggtcttgc cgacacccgc gcctccgaac gcgccaatct tgccgccctt
     1921 cataaagggg cagatcaggt cgacaacctt gatgccggtt tcaaagactt caatcgtggc
     1981 agcctggtct tccagcgtgg gcgcgaggcg gtgaataggc cggcgcggcg cgtcttccac
     2041 ttccggcttg ttatcgactg cctggccaag cacattgaag atgcggccaa gtgtcttcgg
     2101 gccgacgggc acggtaatcg gctggccagt atcaatgacc tcgatacccc gttgtaggcc
     2161 gtcggtcgga cccatcgcta cgcagcgcac ccagttatta cccaggtgct gctctacctc
     2221 aagcgagagg tcatggtccg ccccagcgag acgcacacgc acttcattgt agatttccgg
     2281 gagatgatcc tgtggaaact ccacgtctac aacagctccc agcacctgca cgactgtgcc
     2341 ttttgtcagt gattgtgttg tcatatactt tctcccat
//
LOCUS       DASZAK010001131         1536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_101573, whole genome shotgun sequence.
ACCESSION   DASZAK010001131 DASZAK010000000
VERSION     DASZAK010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101573"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1070
                     /locus_tag="VGT44_20055"
     CDS             111..1070
                     /locus_tag="VGT44_20055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583165.1"
                     /translation="MNTEITTETVPLLQVLDVSKYFGSVIALKDISMTLHAGEVMCLL
                     GDNGAGKSTLIKILSGVHQPSEGHYLFEGKEVHFSSPRDALSVGIATVYQDLAMIPLM
                     GISRNFFLGSEPTQGWGPFSRYNAAFADRVTREELAKIGIQVRETSQPVGTLSGGERQ
                     SVAIARAVYFGAKVLILDEPTAALGVKEAGIVLRYIAQARARNLAVIFITHNPHHAYP
                     VGDRFTILNRGRSYGTFAKSEISREELTGMMAGGEALEELSHELAEFARTDEKARAAL
                     AEAAQQFKVQAQELHVEQLPSKFEKAEQQYADQLPPADGEGKQ"
     gene            1067..>1536
                     /locus_tag="VGT44_20060"
     CDS             1067..>1536
                     /locus_tag="VGT44_20060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2583166.1"
                     /translation="MIQDGQLLNYIGGRWQRSRTSEYLDVRNPATAETMVRVPLSSRD
                     EVDEAVRAAQAAFAGWRRTPPVERIQYLFKLKRLLEEHFDEIARLTTQECGKTLAESR
                     GELQRGLENVEVATGIPALMMGYNVEDIASGIDEHMLRQPVGVVAAITPFNFPG"
BASE COUNT          330 a          436 c          485 g          285 t
ORIGIN      
        1 gtcttcgcag gcgtaaatgc tgagtggtac aaggctgtca ttggaataat actgttgctc
       61 gctgttctgc tcaacagcta tgtgcgcaaa ctggcaatgg aggcgcgacg atgaatacag
      121 agataacgac ggaaaccgtt cccttgctcc aggtgctaga tgtctcgaag tactttggca
      181 gcgtgattgc gctgaaagat atctccatga ccctgcatgc gggcgaggtg atgtgtctac
      241 ttggcgacaa cggcgcgggc aaatccacgc tgatcaaaat cctgtccggc gtgcaccagc
      301 ccagcgaggg ccactacctg tttgagggga aagaagtgca tttttcctct ccgcgcgatg
      361 cgttgagtgt aggtatcgct accgtctatc aggacctggc gatgatcccc ttgatgggca
      421 tttcgcgcaa cttctttctc ggttcggagc caacacaggg ctgggggcca ttcagccggt
      481 acaatgcagc attcgccgac cgcgtgacgc gcgaggaact ggcaaagata ggtatccagg
      541 tgcgcgagac gtcgcaaccg gtcggcaccc tgtccggtgg cgagcgccag tccgtcgcga
      601 ttgcgcgcgc ggtgtacttt ggggccaaag tcttgattct cgacgagcca acggctgcgc
      661 tgggtgtgaa agaggcgggc atcgtcctgc gctatatcgc acaggcgcgt gcgcgcaacc
      721 tggcggtgat ctttatcacg cacaacccgc atcacgccta tccggtcggc gaccgcttca
      781 cgatcctcaa tcgtgggcgc agctatggca cgttcgccaa atccgaaatc agccgcgagg
      841 agctgactgg gatgatggcc ggaggcgaag cgttggagga actgagccac gagctggccg
      901 agttcgcacg cacagatgag aaggcacgcg cagcactggc ggaagcagcg cagcaattta
      961 aggtgcaggc gcaagagctg catgttgagc agcttccgtc gaaattcgag aaggcggagc
     1021 aacaatacgc cgatcagtta ccgccagcag atggagaggg taagcaatga tccaggatgg
     1081 acaactgctc aactatatcg gcggcaggtg gcagcgctcg cgcaccagtg aatatctcga
     1141 cgtgcgcaac cctgcgactg ccgagacgat ggtgcgcgtg ccgctttcat cgcgtgacga
     1201 ggtcgacgaa gcagtacgcg ccgcgcaggc agccttcgcc ggctggcgga gaacgccacc
     1261 agtcgagcgc atccagtacc tcttcaagct caagaggttg ctggaggagc acttcgacga
     1321 gattgcgcgc ctgacgacgc aggaatgcgg caagacgctc gccgagtcgc ggggcgaact
     1381 gcagcgcggt ctcgaaaacg tcgaggttgc gaccggcatc ccggcgctga tgatgggcta
     1441 caacgtcgaa gatatcgcta gtggcatcga cgagcacatg ctgcgccagc ccgtcggcgt
     1501 cgtggcggcc atcactccct tcaacttccc gggcat
//
LOCUS       DASZAK010001132         2025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_527518, whole genome shotgun sequence.
ACCESSION   DASZAK010001132 DASZAK010000000
VERSION     DASZAK010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527518"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2025
                     /gene="pheT"
                     /locus_tag="VGT44_20065"
     CDS             <1..>2025
                     /gene="pheT"
                     /locus_tag="VGT44_20065"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV2583167.1"
                     /translation="DAETILVGRIVSVQPHPNADRLVLVEVDYGAGAPEQVVTGAPNL
                     FQYKGMASLPTLKVAFARAGAVLIDAYSDEQPRPKKKLKPSKIRGVASNGMVCSEREL
                     GLSEEHEGILLLPEDAPVGVPLRDYLGDKVLSLGLTVDMARCLNMIGVAREVSALTGA
                     ALHMPPDELALTDGQDAGQYVAVEIDDSDLCNRYTGMVIRDVQIGPSPRWMQDRLNKA
                     GMRPISNVVDISNYVMLEWGQPTHAFDYDLLVERARRSGASKPVIIVRRAREGEKLTT
                     LDGVERELDDTMLLITDTLGPIAIAGVMGGAETEVHDGTRNVLLESATFDNISNRRTA
                     QKLRLHSEASQRFTRGIPAGLNPIGARRAAALMRDYAGGQVVPGMVDTYPVPQATRVA
                     YTTESNVRRLLGIDVSLDTIAASLARLDITSEWRQRLPASREELGSAAFGLHIEADEP
                     LLRCTAPWHRLDIQVPADLAEEVARVVGYETIPMTLMVDALPTQHRNLPLETDEKIRD
                     ILAGCGLQEIISYSLTTPENHDKVMRQPLGYGEEELHVPFVTLLNPLSPKRRVMRRDM
                     LVSAMENLAYNYRYTQRYAVFEIGRVYWPETGDGVRPDEDPRLCILLTGPRRPSSLYP
                     DPAGTENFDFFDLKGVIETMLQRLGFDPGGVEYVPEGHRAYTATCAWIKL"
BASE COUNT          384 a          604 c          677 g          360 t
ORIGIN      
        1 ggacgcggag accatcctgg taggccggat tgtctcggtg cagccgcatc caaacgcgga
       61 ccggctcgtg ctggtcgagg tagattatgg cgcgggcgca ccggagcagg tcgtcacggg
      121 cgcacctaac ctgttccagt acaagggcat ggcgtcgctg ccaaccctga aagttgcctt
      181 cgcgcgtgcg ggtgcggtgc tgatcgatgc gtatagcgat gagcagccca ggccgaagaa
      241 gaagctcaag ccatccaaga ttcgcggcgt tgcctcgaac ggcatggtgt gttcggagcg
      301 cgagctgggt ctgagcgagg aacacgaggg catcctgctc ctgccggagg acgcgcctgt
      361 gggtgtgccc ttgcgcgatt acctggggga caaggtgctg tcgctgggcc tgacggtcga
      421 tatggcgcgc tgcctgaaca tgatcggcgt ggcgcgcgaa gtgtcggcgc tgaccggcgc
      481 tgcgctgcac atgccacccg acgagctggc gctgacggac ggccaggatg cggggcaata
      541 cgtagcggtc gagattgatg actctgacct gtgcaaccgc tataccggta tggtgatccg
      601 cgatgtgcag atcggcccgt cgccgaggtg gatgcaggat cgtctcaaca aggccgggat
      661 gcgtcccatc agcaacgtgg tggacatcag caactatgtc atgctggaat gggggcagcc
      721 gacgcatgcc ttcgactatg atctgctggt cgagcgggcg cggcgttcgg gcgcgagcaa
      781 gccggtcatc atcgtgcggc gcgccaggga gggcgagaag ctgacgaccc tggatggcgt
      841 ggagcgcgag ctggatgaca ccatgctgct gattaccgat acgcttggcc ccatcgccat
      901 cgcaggcgtg atgggcggcg cggagaccga ggtgcatgat ggcacgcgca acgtgctgct
      961 ggaatcagcc accttcgaca acatcagcaa tcgccgtacc gcgcagaaac tacggctgca
     1021 cagcgaggcc agccagcgct tcacgcgcgg cattccagcc gggctgaacc ccatcggcgc
     1081 gcggcgggcc gccgctctga tgcgcgatta tgccggtggg caggtcgtgc cgggcatggt
     1141 ggatacctat cctgtgccgc aggcgacgcg cgtggcctat acgaccgaaa gcaatgtgcg
     1201 ccgcctgctg ggcatcgatg ttagcctgga caccatcgcc gccagcctcg cgcgattgga
     1261 tatcaccagc gaatggcgcc agcgtttgcc cgcttctcgc gaagagttgg gcagcgccgc
     1321 gttcgggctg catatcgagg cagacgagcc gctgctgcgc tgcaccgcgc cctggcaccg
     1381 cctggacatc caggttccgg ctgacctggc ggaggaagtc gcgcgcgtgg tcggctatga
     1441 gaccatcccg atgactctga tggtcgatgc gctgccgacg cagcatcgca atctgcccct
     1501 ggaaaccgac gaaaagatac gcgatatcct ggcgggctgc gggctgcagg aaatcatcag
     1561 ctattcgctc accacgccgg aaaaccatga taaagtgatg cgtcagccgt tgggatatgg
     1621 cgaagaggaa ctgcatgttc cgttcgtcac actgctcaat ccgttgtcac cgaaacgacg
     1681 agtgatgcgg cgcgacatgc tggtcagcgc gatggaaaac ctggcttaca actaccgcta
     1741 cacgcagcgc tacgccgttt tcgagattgg cagggtctac tggcctgaaa caggggatgg
     1801 tgtgcggcca gatgaagacc cgcgcctgtg catcctgctg acggggccgc gccgcccatc
     1861 cagcctttat cccgacccgg ctggcacgga aaacttcgat ttcttcgatc tgaaaggcgt
     1921 gattgagacg atgttgcagc gactcggttt tgatcctggc ggtgtcgaat acgtccctga
     1981 gggccaccgt gcctacaccg cgacctgcgc ctggatcaag ctaaa
//
LOCUS       DASZAK010001133         3650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_420207, whole genome shotgun sequence.
ACCESSION   DASZAK010001133 DASZAK010000000
VERSION     DASZAK010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420207"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1014)
                     /locus_tag="VGT44_20070"
     CDS             complement(82..1014)
                     /locus_tag="VGT44_20070"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2583168.1"
                     /translation="MYDDSNEIADSKANPPSITYTGHSDSIFCIAFSPDTSRIASASR
                     DTTVRIWRISGGEDVLIYREHASSLLSVAWSPDGRFIASGDTVGIIHVWDASNGDTVN
                     AYHGHTRFARCLAWSPDGRYIVSGGDYGDNTAQVWEASTGKLVYKHTGQYRIFAVGWE
                     PAGKHSSRIVSCSFDASVQIWDAFTGKIALTYSQHSGPVFTTAWSPDGGMIASGGQDG
                     HARSWQADTGETVSVYSGHTNAVKALGWSPDGRHIVSGGDDLTLQVWRAATGDRIALF
                     DGYKKWIRSVAWSPDGRYIASVSGRIIQIGANVV"
     gene            complement(1018..1506)
                     /locus_tag="VGT44_20075"
     CDS             complement(1018..1506)
                     /locus_tag="VGT44_20075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEV2583169.1"
                     /translation="MITVGVLTISDSGAIGARQDTSGENIRALVTEQMPDAVISAGAI
                     IPDEREQIEGILREWSDEKRLNLILTTGGTGLAPRDVTPEATRAVLEREAPGIAEAMR
                     AASLQHTPFGMLSRGVAGTRGHTLIINLPGSPKAVRECLECILPVLPHAVNLLIQGPR
                     EH"
     gene            complement(1517..2170)
                     /locus_tag="VGT44_20080"
     CDS             complement(1517..2170)
                     /locus_tag="VGT44_20080"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HEV2583170.1"
                     /translation="MSRSTTLRLTCACGEPLTITVYDYVNVAQDPQLRYTVMVGLLNS
                     AVCPMCGRRTALAHPFIYSDPAHQLLAYVHPRGDAPDDARQLILDRLRETYLNIVDNA
                     GSATNGHNHAHMNGVNGAHNGATEIPPLQVIFGIDQLSDVIDAVLSQHERLGKLALST
                     QSRDTAERAQMLHLARKLALEMQCQVEVEDLPDEYTVWLFGARRQIGAIMRELSPRS"
     gene            2493..3389
                     /locus_tag="VGT44_20085"
     CDS             2493..3389
                     /locus_tag="VGT44_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583171.1"
                     /translation="MIYYGKYFEHSCILDVLMNNIVENTVAGVIVTAISALFALFLHT
                     VGVSTPGVASVALLVLIGLFFLMRRIYPGFIRGLTIWLVRDALRPKVRNAEAESLKEQ
                     VMDLVTREAHGANPGEHYFIHSYPNQDVCESKIQAAFRSAKKVKILTIRGERYFSGAR
                     SILHDICKNKQDEGCTIQVLVLSPGSSHITDALSANLGHSSSERTRRKMQNTLLELRH
                     LEESNHNIQVKCFNETPNFKMLMFDDSMFVSVYIKAKNDRNTNMLRVRREHQVLFTGL
                     ERDFDDLWKRSFAAKDVLDGRV"
     gene            3389..>3650
                     /locus_tag="VGT44_20090"
     CDS             3389..>3650
                     /locus_tag="VGT44_20090"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2583172.1"
                     /translation="MAAEIFATNEELLQDILSSFFEEDIGPIAHAWDFAATHYGAFHH
                     PTGKPYLQYVLRVAKLLVDLGSAPIVVAAAIVFPPLPQYKGFF"
BASE COUNT          850 a          882 c         1041 g          877 t
ORIGIN      
        1 aatgttgtgt aggggcgcaa ttcattgcgc cctcgtcctt gatcgaagcg gcctggcaag
       61 cgggcgcaat gaattgcgcc cctacacaac attcgcgcct atttgaataa ttctaccgct
      121 caccgaggcg atatacctgc catcgggaga ccacgctacc gaccgaatcc atttcttata
      181 gccatcaaat aaggctatac ggtctccggt ggcggctcgc catacctgca aagtcaggtc
      241 atccccgcct gaaacgatgt gcctgccatc gggcgaccag cccagcgctt ttaccgcgtt
      301 cgtgtggcca gaatagacgc tcaccgtctc gccggtatcg gcctgccatg agcgtgcatg
      361 gccatcctgc ccgccggagg cgatcatacc accgtcggga gaccatgccg tggtgaagac
      421 cggcccgcta tgttgcgagt aagtcagggc aatcttgccg gtgaaggcgt cccatatctg
      481 tacgcttgca tcgaagctac atgagacgat gcggctgcta tgcttgccag caggctccca
      541 acctaccgcg aagatacggt actggccagt gtgcttgtat accagcttgc cggttgatgc
      601 ttcccatacc tgggcggtgt tgtcgccgta gtcgccgccg gaaacgatgt agcggccgtc
      661 gggcgaccag gcgagacagc gcgcgaaacg agtgtggccg tgataggcgt tgacagtatc
      721 accgttggat gcatcccaga catgaataat gcctaccgtg tcgccggagg caatgaaacg
      781 gccatcgggc gaccaggcga cagagagcag actggaggcg tgttcgcggt agataagaac
      841 atcttcgccg ccggatatgc gccagatacg cacggtggtg tcgcgcgatg cggaggcgat
      901 gcgcgatgtg tcgggcgaaa aagcgataca gaagatgctg tcgctgtgtc ctgtgtaggt
      961 gatgctggga gggttagcct tgctatcggc tatctcgttg ctgtcgtcat acattggtta
     1021 atgctcgcgc ggcccctgga tcaacaggtt gacggcatgt ggcaggacgg gcaggatgca
     1081 ttcgaggcat tctctgaccg ccttgggact gcccggcagg ttgataatca gcgtgtggcc
     1141 gcgcgtgcct gctacgccgc gcgataacat gccgaaaggg gtatgttgca gactggcggc
     1201 gcgcatggcc tcggcgatgc cgggcgcttc gcgttcaagc acggcgcgcg tggcttctgg
     1261 ggtgacatca cgcggggcga gaccggtgcc gccggtggtg agaatgagat tgagccgctt
     1321 ttcgtcgctc cattcacgca ggatgccttc gatctgctcg cgctcatcgg gaatgatggc
     1381 gcccgccgag atgactgcat ccggcatctg ctccgtcacc agggcgcgga tgttttcgcc
     1441 gctggtatcc tgccgcgcgc ctatcgcgcc gctatcgctg atggtcaata cgccaactgt
     1501 tatcatatgg tttttcctac gaacgggggg agagttcgcg catgattgcg ccaatctggc
     1561 ggcgcgcgcc gaagagccag acggtatact cgtcaggcag gtcctcgact tcaacctggc
     1621 attgcatctc cagggccagt ttgcgggcga ggtgcagcat ctgcgcgcgt tcagcggtat
     1681 cgcggctctg tgtgctaagg gcgagcttgc cgagccgttc atgctggctg agaacggcgt
     1741 cgatgacatc gctgagctgg tcaataccga aaatgacctg caacggcggt atttctgtcg
     1801 cgccgttatg agcaccattc acaccgttca tatgggcgtg gttgtggccg tttgtggccg
     1861 atcctgcatt gtctacaata tttaagtatg tttcgcgcag acgatccagg atgagctggc
     1921 gcgcgtcgtc cggggcgtcg ccgcgcggat gcacataggc cagcaactga tgcgccgggt
     1981 cggagtagat gaacgggtgc gcaagggcgg tacgcctgcc acacatgggg cagacggcgc
     2041 tattgagcag ccccaccatg acggtatagc gcagttgtgg gtcctgcgcg acattgacat
     2101 agtcgtaaac agtgattgtg agcggctccc cgcacgcgca ggtgagtcgt aaggttgttg
     2161 aacgagacat aatcaaaggg cttccaggat aaatgaaaca taaccatctt gaaataagta
     2221 tatcataaca aggcagtatg gatgatactc acaaatcttc tggcttgact gacatttcag
     2281 gaagatatag gaaagacagg tatcagggtt attggaaata aaaagcattg tttcgtccat
     2341 tatagggtgc ttatgaaacc aaagcggcac gtgcattagc gagtttcaca gataccctgt
     2401 agagtattga gtactacatg gttatacgcc agtggctggc tcgtcaatag aattgatttg
     2461 tagtttatat tgcatcagca tagagaagat ttatgatata ttatggtaaa tacttcgagc
     2521 atagttgcat cttagatgtg ctgatgaata acatagttga aaacaccgta gcaggtgtga
     2581 ttgtcactgc aatatctgcg ctatttgcat tatttttgca tacagttggt gtgagtacgc
     2641 caggtgtcgc ttctgtcgca cttcttgtgc tgataggtct atttttcttg atgaggagaa
     2701 tatatcctgg gtttataaga ggattaacca tatggcttgt gagagatgct ctgaggccga
     2761 aagttaggaa tgctgaagct gagagcctga aagagcaagt tatggatctg gtgaccaggg
     2821 aagctcatgg agccaatcct ggcgagcatt attttattca ttcatatccc aatcaggatg
     2881 tatgtgaatc gaagatacaa gcggcttttc gtagtgcaaa gaaggtaaaa atcctcacca
     2941 ttagaggcga gcgttacttt tcgggcgcca ggagcatatt acacgacatt tgtaagaaca
     3001 agcaggatga gggttgcacc atacaagtgt tggtgctgtc accaggctca tcccatatca
     3061 ccgatgcctt atctgcaaac ttaggacaca gttcatctga acgaacgaga agaaaaatgc
     3121 aaaacacttt gctcgaatta aggcatcttg aggagagcaa tcataacatt caggtaaaat
     3181 gtttcaacga gacacccaac ttcaagatgc tcatgttcga tgatagcatg tttgtaagcg
     3241 tctatatcaa agcaaaaaat gatcgcaaca caaatatgct tcgtgtcagg cgagaacacc
     3301 aggtactttt cactggactt gagcgtgact ttgacgattt gtggaagcgg tcctttgcag
     3361 caaaagatgt acttgatggg agggtttgat ggcggctgaa atttttgcga caaatgaaga
     3421 gttactccag gatatactga gcagtttttt tgaggaagat attggcccta ttgcgcatgc
     3481 ctgggatttt gcggcgacac attatggcgc ttttcaccat ccaacgggaa agccatatct
     3541 gcaatatgtg ctgcgagtgg caaagttact ggttgatctt ggctccgcac caattgttgt
     3601 agctgctgct atcgtgtttc ctccattacc acaatataaa gggttttttc
//
LOCUS       DASZAK010001134         3654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_597862, whole genome shotgun sequence.
ACCESSION   DASZAK010001134 DASZAK010000000
VERSION     DASZAK010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597862"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1261
                     /locus_tag="VGT44_20095"
     CDS             <1..1261
                     /locus_tag="VGT44_20095"
                     /inference="COORDINATES: protein motif:HMM:NF024303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HEV2583173.1"
                     /translation="ARIRDRIAGEFDMTFADEAYARSLAQLRRCLSPAGFLGSPNDID
                     NYARVWARDGIIAGLAAIASGDASLVDGMRSTLLTLARHQGPHGEIPSNVTADGGKVS
                     YGHLVGRVDAPLWYVIGVCAYVHHTGDMVYAASALPGVERALFLVGCWEFNNRGLLYT
                     PLSGNWADEFILEGYLLSDQLLYAMALRNAGVVFQRPAWTDKAASLLALLEVNYWPRF
                     DARDDALVYHPHAYRAYLDAHGEPLHWLAAFSPGGYADRFDGLAHALALLAQLGGDAQ
                     RQQAIAYVQQLSEQIGSELLPAFWPVIQPGDLEWQLLQANHLYGMLKNQPFTYHNGGL
                     WAVVTGLYAVGLACCGQRERAGQLLHAINEANARGRDGQQWEFAEYYHAQTHMPMGTS
                     HVDWSAAAGILAHQAMRQDKVASLFFH"
     gene            1382..3571
                     /locus_tag="VGT44_20100"
     CDS             1382..3571
                     /locus_tag="VGT44_20100"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HEV2583174.1"
                     /translation="MNSGKSIHLGLVLHNHQPVGNFPWVFQQVYEESYLPMIEALERH
                     PHVRLSLHYTGSLLDWMQEEHPEFLERIGALVRRNQVEMVSGGYYEPIFPSIPGRDRL
                     AQIQRLSAWLTEHFDVHATGMWIAERVWEPEMPRLLHEAGIAWTVLDDVHFKNVGLED
                     SDLYGYYVTEDQGKAVKVFATSQAMRYTIPWRPAAETIATLRGLATYDGRRIAVMGDD
                     GEKFGSWPGTHEYCWGDKSKSGWIEEFFTLLDENRAWLHTIPPGEYSQSYPALGRIYI
                     PTSSYIEMMEWALPPKKSYTFGKLLHQLQDEHRTDLLQFMRGGFWRNFLVRYSEVNIQ
                     HKKMLRVHDAVYRAGANVENGAVDLWKAQANDTYWHGLFGGIYMGHVRSAIFHHLIKA
                     QNAADTAVMGKEHWQRYEFADFDCDSQEELLLEGDLQNLYIDPQRGGTLFEWDMRRSA
                     HNLLCVMMRREEGYHQVLRDFERERRRFAAQQAATDRQGAHDEVVSPHAAVKVKEPDL
                     DRHLVVDRYRRHSLIDHFFDAGTSLSTFAQMQYEEYGTFVEQPYATHARQGDDGLTVT
                     LTREGHVRRGGALAPVPVRITKSLIAPLGEERLVVRYTIENRGQARLQTRFGCEWNMH
                     LLGGGANDQAYYAIDGHTLENSHFDSTGEVKDVISFHVGNSWIGQDVGFTLDRPATLW
                     RFSIETVTGSEAGFERNHQGSCMTLLWPLVLEAGQSWQAEIRCETGE"
BASE COUNT          724 a         1085 c         1108 g          737 t
ORIGIN      
        1 tgcaaggata cgagatcgca ttgccggaga atttgatatg accttcgctg atgaagccta
       61 cgcgcgttcc ctcgctcagc tgcggcgctg cctttccccg gctggctttc ttggctcccc
      121 caacgatatt gataactacg cccgtgtctg ggcgcgcgat gggattatcg ctggcctggc
      181 agcaatagcg agcggcgatg cttctctggt cgatggaatg cggagtaccc ttctcacgct
      241 tgcccggcac caggggccgc acggggaaat accaagcaac gtcaccgccg atggaggcaa
      301 ggtgagctac ggacacctgg tgggccgcgt tgacgcgccg ctctggtatg tcatcggcgt
      361 ctgtgcctat gttcatcata ccggcgatat ggtctacgcc gcgtctgcgc ttcccggcgt
      421 agaacgcgcg ctgttcctcg tgggctgttg ggaattcaat aatcgcggac tgctgtatac
      481 tcctctctct ggcaactggg ctgacgagtt catcctggaa ggctatcttc tctccgacca
      541 gctactgtac gcgatggcgc tgcgcaacgc tggagtcgtg ttccagaggc cagcgtggac
      601 ggacaaggcg gcctcactgc tggcgctgct ggaggtaaat tattggccca ggttcgatgc
      661 gcgtgatgat gcgctggtct atcatcccca tgcctatcgc gcgtatctcg acgcgcatgg
      721 cgaaccgctc cactggctgg ctgccttctc accaggcggc tacgcggatc gcttcgatgg
      781 cctggcgcac gcgctcgcgc tgctcgcgca gttgggaggc gacgcacagc ggcagcaggc
      841 catagcgtat gtacagcagt tgagcgagca gattggcagc gagctgctgc cagctttctg
      901 gccggtgatc cagcccggtg atctcgaatg gcagttgctc caggccaatc acctctatgg
      961 gatgctaaaa aaccaaccgt ttacctatca taatggagga ctgtgggcgg tcgtcacggg
     1021 gttatacgca gtcggcctcg cgtgctgcgg ccagcgtgaa agagccgggc aactgctcca
     1081 cgccataaac gaggcgaacg cgcgtggaag agacggccag cagtgggagt ttgccgagta
     1141 ttaccacgcg caaacgcaca tgccgatggg aaccagtcac gtcgattgga gcgccgccgc
     1201 cgggattctg gcgcaccagg ccatgcggca ggataaggtc gcctcgctct tcttccatta
     1261 gtacatggaa gatggacagt tgacacagtc agaaccctgg caagcttgac ccctcttgtt
     1321 tcattacgat atagtggatg caatccaatc ttattttcgg gtgaggtcta agacaatggt
     1381 aatgaatagt ggtaagagca tccacctcgg tttggtcctg cataatcacc agccggtcgg
     1441 gaatttcccc tgggtgttcc agcaggtgta tgaagagtcg tatttgccga tgatcgaggc
     1501 gttggagcgc catccccatg tgcggctctc gctgcactac accggctctc tgctcgactg
     1561 gatgcaggag gagcaccctg aatttctgga gcgcatcggc gcattggtca ggcgcaacca
     1621 ggtagagatg gttagcggcg gctactacga gccgattttc ccttcgatcc ccggtcgcga
     1681 ccggctggcg caaatccaac gcctttccgc gtggcttacc gagcactttg atgtccatgc
     1741 caccggcatg tggattgcgg agcgtgtctg ggagccggag atgccccggc tgctgcacga
     1801 agctggcatc gcctggacgg ttctcgacga tgtgcatttc aaaaatgtcg ggttggagga
     1861 tagcgacctg tacggctact acgtcaccga ggatcagggc aaagctgtca aggtcttcgc
     1921 aaccagccag gccatgcgct acaccattcc ctggcgaccc gccgctgaga ccatcgccac
     1981 gctgcgcggg ctggccacgt atgatggcag gcgcatcgcc gtgatgggcg acgacggcga
     2041 aaagttcggc agctggcccg gaacgcacga gtactgctgg ggtgacaaaa gcaaaagtgg
     2101 ctggatcgag gaatttttta ccctgcttga cgagaatcgc gcgtggctgc atactattcc
     2161 gcccggcgag tatagccagt cctatcccgc gcttggacgc atttacatcc ctacctcgtc
     2221 ctatattgaa atgatggagt gggcgctgcc ccccaaaaag tcgtatacct tcggcaagct
     2281 cttgcaccag ttgcaagacg agcatcgcac cgacctgctg caatttatgc gcggcggctt
     2341 ctggcgcaat ttcctggtgc gctacagcga ggtcaatatc cagcacaaga aaatgctgcg
     2401 cgtgcatgat gctgtctacc gcgcgggcgc gaacgtggaa aacggcgcgg tagacctgtg
     2461 gaaggcgcag gccaacgata cttactggca tggactcttc ggcggcatct acatggggca
     2521 tgtgcgctcc gccatcttcc atcacctcat caaggcgcaa aatgccgccg acaccgccgt
     2581 gatgggcaag gaacactggc agcgttacga gttcgccgat ttcgactgcg atagccagga
     2641 ggagttgctg ctcgaaggcg acctgcaaaa cctctatatc gatccgcagc gcggagggac
     2701 gcttttcgag tgggatatgc ggcgctccgc gcataatctg ctgtgcgtga tgatgcgccg
     2761 cgaggagggt tatcaccagg tactgcgcga tttcgagcgc gagcgccgcc gcttcgccgc
     2821 tcagcaagca gctacagaca ggcaaggcgc gcatgacgag gtcgtctcgc cgcatgcggc
     2881 ggttaaggtc aaggagcctg acctggatcg ccatctcgtc gtggatcgct atcgccgtca
     2941 tagcctcatc gatcacttct tcgatgccgg taccagcctc tcgacgtttg cgcagatgca
     3001 atacgaagaa tatggcacct ttgtcgagca gccctatgcc acccacgcgc ggcagggcga
     3061 cgacggtcta accgtcacgc tcacccgcga aggccatgtg cggcgcggcg gcgcgcttgc
     3121 ccctgtcccg gtccgcatca cgaaatcgct gatcgcgccg ctcggcgaag agcgcctggt
     3181 cgtgcgctac accatcgaaa acaggggaca ggcgcgcctg caaacgcgct tcggctgcga
     3241 atggaatatg catctgctgg gaggcggcgc gaacgaccag gcctactatg ccatcgatgg
     3301 ccacacgctc gaaaacagcc atttcgacag caccggcgag gtgaaggacg ttatatcttt
     3361 ccatgttggc aattcctgga taggccagga tgtcggcttc accctcgacc ggcccgccac
     3421 actctggcgc ttctcgattg agaccgtcac cggctcggaa gccggcttcg agcgcaacca
     3481 ccagggaagc tgcatgaccc tgctctggcc gctcgtgtta gaggccgggc agtcctggca
     3541 ggcggagatt cgctgcgaaa caggggagtg atcgggcgac cgcaaaggta tgccgccagt
     3601 ggtgttctcc gaaggcacct ccctctgtca tgctgagcgc agcgaagcat ctac
//
LOCUS       DASZAK010001135         1530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_312839, whole genome shotgun sequence.
ACCESSION   DASZAK010001135 DASZAK010000000
VERSION     DASZAK010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312839"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="VGT44_20105"
     CDS             complement(<1..358)
                     /locus_tag="VGT44_20105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583175.1"
                     /translation="MKIIRHYRLFSWLSGILLIGLLAACGTTQPTTNGPTPTSPAGTT
                     PGATATLSGGHPPVQTPTTAPEPPTQTNCPATGTARAAVLANLALGNHPNIVYITDLY
                     QGATPVPTSAALKRYDV"
     gene            1015..>1530
                     /locus_tag="VGT44_20110"
     CDS             1015..>1530
                     /locus_tag="VGT44_20110"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911257.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV2583176.1"
                     /translation="MVAEEKYVDEIIDQEDNFSQWYNQVVRKAELADYAPVRGCMIIR
                     PYGYAVWENIQRLLDARFKETDVMNAYFPLLIPRSFLEKEAKHVEGFAPELAWVTRGG
                     GEELEEPLAIRPTSETIIGYSYAKWVQSYRDLPILINLWNNVMRWEKRTTLFLRTTEF
                     LWQEGHTAHRNL"
BASE COUNT          350 a          399 c          482 g          299 t
ORIGIN      
        1 tgacatcata gcgtttcagc gcggcagacg tgggaactgg cgtggcgcct tgatacaggt
       61 cggtgatgta gacgatattc gggtgattac caagcgccag gttcgccagc accgccgcgc
      121 gcgctgtgcc ggtcgccggg caattcgttt gcgtgggcgg ctccggcgcg gtcgtgggcg
      181 tctggaccgg cgggtgaccg ccgctcagcg tggccgtcgc tccgggtgtc gtgcctgcgg
      241 gactggtggg agtcggccca ttggtggtcg gctgcgtcgt gccgcaagca gccagcaagc
      301 cgatcagcag aatgccggac agccatgaga agagacgata atggcgaata attttcatat
      361 gaatcttcct ctcaataatg tacagtgacg agttccatcg gccccgtcat aaggacgcga
      421 tgatgaactc gaacgaccaa agtcgataat gtcatcctga gcgcagcgaa ggatctccct
      481 cgtcggcgac ccagatcctg agcgcagcga aggatgacat tccagggttt gttttgaaca
      541 ttcttcatcg cgtccctacg acggaagccg ctggaaggct ccgcccgcag tataacggag
      601 aatcggcggc ggggaaagtg aagcgctcac agctacagcg tgaaaggggt cacatcgcgc
      661 ttgacaggtt ccatgcattc atgtatgctt agcgcagcaa aacacaggga acgcgctgac
      721 cgggattagt aagcgcgggc ggaatccagc agcgagccgg gagagtgcaa gccggtagag
      781 agcgccgcgt tgaacccacc tgagagcgtc agtgacaagc tgaccggaac ctcaccgtta
      841 acggaggcgc agttataacg aaatttgagt gagactgcat aagcggggct gcgtcacgag
      901 gcgcgactca agcaaggtgg taccacgagc aaacatgctc gcccttacag acaagggcga
      961 gtttttttat ggatttttgt cgtgtacaag gttgtatcga gaaaggaaaa ggttatggtt
     1021 gccgaagaaa agtatgttga tgagatcatc gaccaggagg acaacttttc gcagtggtat
     1081 aaccaggttg tgcgcaaggc ggaactggcc gactatgctc ccgtgcgcgg ctgcatgatt
     1141 atccggccat atggctatgc cgtctgggaa aatatccagc gcctgctgga tgcgcgtttc
     1201 aaggaaaccg acgtgatgaa cgcctacttt ccactgctga tcccgcgcag tttcctggaa
     1261 aaagaggcca agcacgtcga gggtttcgct ccggagctgg cctgggtgac gcgcggcggc
     1321 ggcgaggaac tggaggaacc cctggcgatt cgccccacca gcgaaaccat catcgggtac
     1381 agctatgcta aatgggtgca gagctatcgc gatctgccga tcctgattaa cctgtggaac
     1441 aatgtgatgc gctgggagaa gcgcaccacg ctcttcttgc gcacaacgga attcttgtgg
     1501 caggaaggtc atacggcgca tcgcaacctg
//
LOCUS       DASZAK010001136         8509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_457021, whole genome shotgun sequence.
ACCESSION   DASZAK010001136 DASZAK010000000
VERSION     DASZAK010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457021"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VGT44_20115"
     CDS             <1..939
                     /locus_tag="VGT44_20115"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV2583177.1"
                     /translation="QGNYASYDIEPLEAGYGVTLGNALRRVLLSSLPGAAVTSVRVDG
                     VQHEFQDIPNVKEDVTDIVLNVKKLRLRSFSDHPVSMRLEVHGEREVTAADIMAPSTV
                     EIVNPDLYIATLDNDNARLEMELVVETGKGYVPADSKEDQPIGVIPVDAIYTPVQKVN
                     YTVEHTRVGQMTNFDKIVMEVWTDGTITPDEALRQSSDILVRHFTQLSNYHATLAEPE
                     KQPLSSMPIPSKIYDTPIEELDLSVRAYNCLKRSNITKVGQVLSMNEDDLLGVRNFGE
                     KSLQELREKLLVRNFLPNPRINANGADLDGDHEMEE"
     gene            941..1567
                     /locus_tag="VGT44_20120"
     CDS             941..1567
                     /locus_tag="VGT44_20120"
                     /inference="COORDINATES: protein motif:HMM:NF013369.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HEV2583178.1"
                     /translation="MRHRVAGNRMSMPEPRRRSARRNLMAGLFRYDRIKTTESRARAI
                     KGEAEKLLTVAIKGQIAAHNHLASVIEDEEKAAAMLAFARRGRYTLDKHVFTNDERAE
                     IGLAPLTDAGRKFAEKKLKDRRAELLRIISNENEAARALEAAYKAMTIELHARRQILK
                     ALPDELVVKRIFDELAPRYANRHGGYTRIIKLGKRLGDAADMAQISLV"
     gene            1606..2418
                     /gene="truA"
                     /locus_tag="VGT44_20125"
     CDS             1606..2418
                     /gene="truA"
                     /locus_tag="VGT44_20125"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV2583179.1"
                     /translation="MKLACGIEYDGTGYHGFQRQAEMQGPTIQGTLEAAIARICGDST
                     TVYGAGRTDAGVHASGQAIHFDCGAQSRLTPQEWMRAMNAVLPDSITVRWIAVVADSF
                     HARYSAVSRSYRYTIWNDAAPSPLRARYSYHCVRPLAVDAMHEASQFLPGRRDFGAFG
                     RSPDEGNPLKAGPHSSVRTILEASCQRQDALIYFDVTADAFLTGMVRRIVGTLLLVGQ
                     KRLGIDDFATIVQRAEKTHPGSAAPSHGLCLVKVTYPNGLLQWEKMDNEKDI"
     gene            2402..2905
                     /gene="rplM"
                     /locus_tag="VGT44_20130"
     CDS             2402..2905
                     /gene="rplM"
                     /locus_tag="VGT44_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV2583180.1"
                     /translation="MKKTYSAKAADLDPQWYVIDAEGQVLGRLASQIAQILRGKHKPT
                     YTPNLACGDFVVVINADKIAVTRNRLDKKIYYRHSQYPGGLKQTTLRQTLEGRFPERA
                     LRHAVRGMVMHNRLGGQLMSRLKIYSSKEYPEAPHHPHTAQKPVPWTGPEGLLKQRQA
                     AESAAEN"
     gene            2936..3334
                     /gene="rpsI"
                     /locus_tag="VGT44_20135"
     CDS             2936..3334
                     /gene="rpsI"
                     /locus_tag="VGT44_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV2583181.1"
                     /translation="MKGEYYYGMGRRKTAVARVRLFPNGDGSIVINGKNANQYFGPRE
                     SYHETMTAPMRLLELADAYTMTVRVVGGGVTGQAGAIRHAVARALLRVNPEWKPALRK
                     AGYLTRDPRMKERKKPGLKRARKAPQYTKR"
     gene            complement(3718..4692)
                     /locus_tag="VGT44_20140"
     CDS             complement(3718..4692)
                     /locus_tag="VGT44_20140"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEV2583182.1"
                     /translation="MSKANVLFMNGEFIPAEQGVISVRTHGFAYGTGCFEGIRGYWNQ
                     QDEQVYLFRLREHYQRLHNSCKILHITLPYIVEQLIEISIELVRRNGFRQDIYLRPVA
                     YKGDQIIGVRLHNLEDHFIITAEPLGDYINTGGMSVGVSSWRRIDDNAIPSRAKIIGS
                     YVNSALAKTEAMLNGFDEAIMLTQEGHVAEGSAENIFLVINGELVTPPVTENILQGIT
                     RDSIMELARRELGRITRERQIDRTELYTASEIFLCGTGAQIAPVVKVDHRPIGDGTVG
                     PISSAIQSMYFDVVRGRRLEYRQQWCTPVYDVVEERHESSALGTSAEA"
     gene            4980..6632
                     /locus_tag="VGT44_20145"
     CDS             4980..6632
                     /locus_tag="VGT44_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV2583183.1"
                     /translation="MGQVVSYSRKVRPGRRPAVLICLCVLAVVLAACGSGPTTPTSPN
                     LAANQTLIFPNVGTPDIGIMDPALGPDANSAIAVGMVYSGLVKFDKNLNVVPDQATWS
                     ISADRKTYTFTLKPGLKFSDGTPVTAETYVYTLTRALFPEVASPIATFFLGNILGAND
                     VANKKTRVLQGVKALNSTTLQITLIQPAEYFLDIMADSIAFALNENVIDQYGETGWPL
                     EVAGNGIGTGPFMVKEWDHNAKMILVPNPYWYGNKTKLKEVDMIFVNDASTAYKAYQA
                     GQYAFTWNITAQDLSTAKGLSGFTTQSLLQTDLLFFNNTKPPFSHPEVRQAFAYATDK
                     NTLASAIFKGAAVPATTIIPPGMPGYQSGYKGIPYDPAKAKSLLQSVYPDLSKVPTIT
                     FSYPNSQITPLESQALQQMWQNALGIQVKLNPVELTAYNQETATQPPVVQFGFTQWTA
                     DYPDPYDWLYLNLLSTAPNNNGDWHNATFDQTLQQAEQTSGAARIQLYNQAEQIAISN
                     VGWLPLDHQALSAIIPSWVHGVSLNSTGLFFGDWSDVYLLQH"
     gene            6811..8067
                     /locus_tag="VGT44_20150"
     CDS             6811..8067
                     /locus_tag="VGT44_20150"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEV2583184.1"
                     /translation="MAVEMVNPYDVAVAQFDEAADRLGLSQAMRAILRKPKRELIVNF
                     PVRMDNGDVEMFTGYRVQHNINRGPAKGGIRFSPEVSLDEVRALAMWMTWKCAVVGIP
                     FGGAKGGVICDPHHMSRAELERLARRYATEISILIGPDSDIPAPDMNTNPQIMAWIMD
                     TYSMHQGYSVPAVITGKPLAIGGSEGRLEATARGVLVVTREAMHDLGMIPEESAVVVQ
                     GFGNVGSITARLLQDELKCKVVGLSDISGGVYNPRGIDIHRALRHSKEHGTLHGLIDT
                     ESVTNAELLELPCDVLIPAALENQLTGRNAARVKARLVIEAANGPTTPDADVILNDRG
                     VTIVPDIMANAGGVTVSYFEWVQDLQRFFWAEHEINNRLESIMTRSYDAVRQKAAEQD
                     CNLRMGAYLLAVARVAEATEIRGVYP"
     gene            8192..>8509
                     /locus_tag="VGT44_20155"
     CDS             8192..>8509
                     /locus_tag="VGT44_20155"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583185.1"
                     /translation="MMRARPFSLVLSGCCFLLLLAGCGYTQPAGTISVATYTPSANNG
                     GIPEALEWSPDGTRLATAYSSGYIEIWQATTGKRLLVYTGHKVSNRVGGLYTVAWSPD
                     GNSI"
BASE COUNT         1886 a         2425 c         2457 g         1741 t
ORIGIN      
        1 cagggaaact atgcgagcta tgatattgag cctttagagg cggggtatgg agtgactctg
       61 ggcaacgcgc tgcgtcgcgt gttgctctcc tcgctgcccg gagcggcggt gacttctgtc
      121 cgcgttgatg gggtacagca cgaatttcaa gacatcccca atgtcaagga agatgtcacc
      181 gatatcgtgc tgaatgtgaa gaagctgcga ctgcgctcct tctcggacca tcccgtctca
      241 atgcgcctgg aggtgcatgg cgagcgcgag gtgacggcgg cggatatcat ggctcccagc
      301 acggtggaga tcgtcaaccc cgatctttat attgccaccc ttgataatga caatgcccgc
      361 ctcgaaatgg agctggttgt tgagaccggc aaaggctatg tgcctgccga ctcgaaagaa
      421 gatcagccca tcggggtgat acccgtcgat gctatctata cgccggtgca aaaggtcaac
      481 tataccgttg aacacacacg tgtagggcag atgacgaact ttgataaaat tgtgatggaa
      541 gtctggacgg atggcacgat tacgccggat gaagctctgc gacaaagctc tgatatcctg
      601 gtgcgccact tcacgcagct ttcgaactac catgcgacac tggccgaacc ggagaagcag
      661 ccactgagta gcatgccaat cccgtcgaag atctacgata ctcccattga ggagcttgat
      721 ctttcggtgc gcgcctacaa ctgcctcaaa cgcagcaaca ttacgaaagt gggccaggtg
      781 ttgtcaatga atgaagatga cctgctgggt gtgcgcaatt tcggtgaaaa gagcttacag
      841 gagcttcgcg agaagctgct ggtacgcaac ttcttgccga atccgcgcat caacgcgaat
      901 ggggccgatt tagacggcga tcacgaaatg gaggaatgag ttgaggcacc gagttgccgg
      961 taatcggatg agtatgcctg agccgcgccg ccgctccgcg cgtcgcaatt tgatggctgg
     1021 ccttttccgt tacgaccgca taaaaacaac cgagtcgcgc gcgcgcgcca ttaaaggcga
     1081 agcagaaaaa ctgcttaccg tcgcgattaa agggcaaatt gctgcccaca atcacctggc
     1141 ttccgtcatc gaagacgagg agaaggctgc tgcgatgctc gccttcgccc gcagaggccg
     1201 ctatactctg gataaacacg tcttcacgaa cgatgagcgc gctgaaattg gccttgcgcc
     1261 cctgacggat gccggtcgca agtttgccga gaagaagctg aaagatcgac gcgccgagct
     1321 gctgcgcatc atctcgaacg aaaatgaggc agccagggct ctggaggcgg cttacaaggc
     1381 gatgaccatc gagttgcacg cccgccgtca gattctcaaa gcgctgcccg acgagctggt
     1441 cgtcaagcgc atcttcgacg agctggctcc gcgctacgca aatcgacatg gcggttatac
     1501 ccgcatcatt aagcttggca aacgcctggg tgatgccgcc gacatggccc agatttccct
     1561 ggtgtaggga cgcgatatat cgcgtcccta ttttggcaga agattatgaa acttgcctgc
     1621 ggcattgaat acgacggaac cggctatcac ggctttcaac gccaggcgga gatgcagggg
     1681 ccgacgatcc agggaacact ggaggcggct atcgcgcgta tctgcggcga ctcgacaacg
     1741 gtgtacggcg cgggccgcac ggatgccggg gttcacgcca gtggccaggc gatccacttc
     1801 gattgcggcg cgcaatcgcg gttaacgccg caggagtgga tgcgagccat gaatgcggtc
     1861 ctgccggaca gcatcacggt acgctggata gcggtggttg ctgatagctt tcatgcccgc
     1921 tacagcgcgg tgagccgctc ctatcgctat acgatctgga acgacgcggc tccgtcgccg
     1981 ttacgggcgc gctacagcta ccattgcgtc aggccgctcg cggttgacgc gatgcatgaa
     2041 gcgagccagt tcttgcctgg acgaagagac tttggagcct ttgggcgcag tcccgatgag
     2101 ggcaatccgt tgaaagcggg gccgcattcc agcgtgcgca ccattctaga agcctcttgc
     2161 cagcgccagg atgcacttat ctattttgat gtgactgcgg acgccttcct gacgggcatg
     2221 gttcgcagga ttgttggaac actgctgctc gtagggcaga agcgactggg catcgacgac
     2281 tttgccacga tcgtgcaacg cgccgagaag acgcacccag gttcagcggc tccatcacac
     2341 ggtctatgcc tggtaaaagt tacgtatcct aacggtttgt tacaatggga gaaaatggac
     2401 aatgaaaaag acatatagcg cgaaagccgc cgatctggat ccccagtggt atgtcatcga
     2461 tgcggaaggg caggtactcg gtcgcctggc atcgcagatt gcgcagattc tgcgcggcaa
     2521 gcacaagccg acctacacac cgaatcttgc ttgcggcgac tttgtggtcg taatcaacgc
     2581 ggacaaaatc gccgtgaccc gcaaccgcct ggacaagaaa atctactacc ggcacagcca
     2641 gtaccccggc ggtctgaaac agacgacgct gcgccagacg ctcgaaggcc gcttcccgga
     2701 gcgcgccctg cgacacgctg tgcgcggtat ggtgatgcat aatcgcctgg gcgggcagtt
     2761 gatgtcgcgc ctcaaaatct attccagcaa ggaatatccg gaggccccac atcatcccca
     2821 tacagcgcag aagcctgtac cctggacagg gccggaggga ttgttgaaac aacggcaggc
     2881 cgccgaaagc gcggcggaga actaaagaag aggagaatcg acagtggccg ataccatcaa
     2941 aggcgagtac tactatggca tgggacgacg caaaacggca gtggcccgcg tgcgcctctt
     3001 ccccaacggc gacggcagca tcgtcatcaa cggcaagaac gcgaaccagt attttggccc
     3061 ccgcgagtcc taccacgaaa ccatgactgc cccgatgcgc ctgcttgaac tggccgacgc
     3121 ctacaccatg actgtgcgtg tcgtcggcgg cggcgtcacc ggccaggccg gcgcaatccg
     3181 tcacgcggtg gcgcgcgccc tgctgcgcgt caacccggaa tggaaacccg ccctgcgcaa
     3241 agccggctac ctcacccgtg acccgcgcat gaaggagcgc aagaagcccg gcctcaaacg
     3301 cgctcgcaaa gctccgcagt acacgaagcg ctaagcgttt cgataggccg tacccaaatg
     3361 tcgtaaagag cgaggaggac gagggcgacc cttgcggtcg ccctggaact taataccaat
     3421 gttcgtagta atttatcatt gcatattatt gccttccaca gatcgattgt tgtcattcca
     3481 tctaacgaat atcccgcaag agcagcccct gaaacatacg tcgctcggtc gccggggtag
     3541 cattcataaa acgtgttctc ctgtcaggcg cattggcata gaaatttgcc agtcaggccg
     3601 tcctcagaaa gaaaagcata tctcccaggt attttgccag agcagaaaag aagcagccgc
     3661 ccagaaaaaa tcgctgagcg gctgtgtatg cattttatct ggcggaagct ggcttcctta
     3721 cgcttccgcg ctcgtcccaa gcgcgctcga ctcgtgccgc tcctccacca cgtcgtaaac
     3781 gggcgtacac cactgctggc ggtattcgag gcggcgaccg cgcacgacat cgaagtacat
     3841 ggactggatc gcgctgctga tcgggccgac ggtgccgtcg ccgatggggc gatgatcgac
     3901 tttcacgacg ggggcgatct gcgcgccggt gccacacagg aagatttcgc tggcggtata
     3961 gagttcggtg cgatcaatct gcctctcgcg cgtgatacga ccaagctcgc gacgggccag
     4021 ttccatgatg gagtcgcgcg tgatgccctg caggatattt tctgtaaccg ggggggtgac
     4081 gagttcgcca ttgatgacca gaaagatgtt ctccgcgctt ccctcggcaa catgcccttc
     4141 ctgggtcagc atgatggcct cgtcgaagcc gttcagcatc gcctcggtct tggcaagcgc
     4201 cgagttgacg taggagccga tgatcttggc gcgcgagggg atggcgttgt cgtcgatgcg
     4261 cctccacgag gagacgccaa cgctcatacc acccgtgttg atataatcgc caagcggctc
     4321 agccgttatg atgaagtgat cttccaggtt gtgcaggcgc acaccgatga tctggtcgcc
     4381 cttgtacgcg acgggccgca ggtagatgtc ctgccggaaa ccgttgcgcc gcacaagctc
     4441 gatgctgatt tctatcagtt gttcgacgat gtaggggagc gttatatgca ggattttgca
     4501 ggagttgtgg agtcgctggt aatgctcgcg cagccggaac aggtacacct gctcatcttg
     4561 ctggttccag tagccgcgaa taccttcaaa gcaaccggtt ccataggcga aaccgtgcgt
     4621 cctgacagaa atgacacctt gttcggcggg gataaactcg ccgttcataa acaaaacgtt
     4681 tgccttcgac atgctgcttc ctcttgctct tcattgaaca gaacggtaat gagcttccat
     4741 ccctgttctc cattgaacag aacgatgctg tatgttcatt ataggctgtc gggagggaga
     4801 tgtcgagagt caatatagac aagacaggga aagccaatgc tgccatgatg tgaagtatta
     4861 tcccggcagg gcgtaattca gttagaaagc tccccttgca gtcaccgtgg gacggtcata
     4921 caatagcggt aatatctcat gcgtactcgt tggcttcatc tcagaaagtg aggcacctca
     4981 tgggtcaagt tgtttcatat agtcgcaagg tgcggccagg acgccgcccg gcagtgctta
     5041 tttgtttgtg cgtcctggct gtcgtcctgg ccgcgtgtgg cagtggtccc accacaccta
     5101 ccagtccgaa cctggcggca aaccagacgc tgatctttcc caacgtcggt acccctgata
     5161 tcggcatcat ggacccggcg ctgggaccgg atgccaattc ggccatcgct gttggcatgg
     5221 tttacagcgg cctggtgaag ttcgataaaa acttgaatgt cgtaccagac caggccacct
     5281 ggtcaatttc ggcagaccgg aaaacctata cgttcaccct caagcccggt ctcaagttct
     5341 ctgatggcac gccggtcacg gcggagacct atgtctacac gctgacacgc gcgttgttcc
     5401 ctgaagtagc atcgccgatt gcgacgtttt tcctggggaa tattttgggc gcgaacgatg
     5461 tggccaacaa aaagaccagg gtactgcaag gcgtcaaggc gctcaatagc accactttgc
     5521 agatcactct gatccagccc gccgagtact tcctggatat catggccgac tcgattgcct
     5581 tcgcactgaa tgagaatgtg atcgaccagt atggtgaaac aggctggcca ttggaagtgg
     5641 ccgggaacgg tattggcacc ggtccattca tggtcaaaga atgggatcac aacgctaaaa
     5701 tgatactggt gccgaatccc tactggtacg gcaataagac gaagctgaag gaagttgata
     5761 tgatcttcgt caacgatgct tcgacggcgt acaaggcgta ccaggccggt cagtatgcgt
     5821 ttacctggaa cattaccgcc caggatttga gtaccgcgaa agggctatcc ggttttacga
     5881 cgcagtcgct gttgcagacc gacctgctct tctttaataa caccaaaccg ccattcagtc
     5941 acccggaggt gcgccaggcg ttcgcctacg ccaccgacaa gaacacgctg gccagcgcca
     6001 tcttcaaagg ggcggcagta cccgctacca ctatcattcc accggggatg cctggctatc
     6061 agtcaggtta caagggaata ccgtatgatc cagccaaggc gaagtcccta ttgcaatcgg
     6121 tctaccccga cctgagcaag gtaccaacga ttaccttctc gtaccccaac tcgcagatca
     6181 cgccgcttga atcgcaggca ttgcagcaaa tgtggcagaa tgccctgggc attcaggtca
     6241 agctgaaccc ggtcgagtta accgcctata accaggagac ggccacgcag ccgccggtcg
     6301 tccagttcgg cttcacgcag tggacggcgg actatcccga cccgtatgac tggctgtacc
     6361 tcaatctgct ctcaaccgcg cccaacaata acggtgactg gcataacgcc acgttcgacc
     6421 agacgctgca acaggctgaa caaacgtcgg gcgcggcgcg tatccagctc tacaaccagg
     6481 ccgaacagat cgccattagc aatgttggct ggctgccgct ggatcaccag gcgctctccg
     6541 ccatcatccc gtcctgggtg catggcgtca gtttgaatag caccggcctc tttttcggcg
     6601 actggtcgga tgtgtacctg ttgcaacatt agcagccttg cctcgaccat tcatcaggtg
     6661 atatactttc ttcagaaagg gaatgcccgc cgcgaggaaa tcaggcgggc tttcttgtat
     6721 aggcgcacac cttcaagtta tcattgaagc aacgatgctt ctactgctgt gttgcataat
     6781 gaccaatgcc tggtcaagga ggtatttaca atggctgttg aaatggttaa tccgtatgac
     6841 gttgctgtcg ctcaattcga tgaggctgct gaccgcctgg ggctttcgca ggcgatgcgc
     6901 gcgatcttgc gcaaacccaa acgcgagctg atcgtcaatt tcccggtgcg tatggacaac
     6961 ggcgatgtgg agatgttcac cggctaccgc gttcaacaca atatcaatcg cggccctgca
     7021 aaaggcggta ttcgtttcag ccccgaggtt tcgctcgacg aggtgcgcgc gctagccatg
     7081 tggatgacgt ggaagtgcgc ggtggtcggc attccctttg gcggcgcgaa gggcggcgtg
     7141 atctgcgacc cccaccatat gtcgcgcgcc gaattggagc ggctcgcgcg ccgctatgcc
     7201 acggagattt cgatattgat cggccccgat agcgatattc ccgcccccga tatgaatacc
     7261 aacccacaga ttatggcctg gattatggac acctactcca tgcatcaggg ctattcggtc
     7321 cccgccgtga ttaccggcaa gccgctggcc atcggcggca gcgagggtcg cctggaagcc
     7381 accgcacgtg gcgtgctggt ggtgacgcgc gaggcgatgc acgatctcgg catgatcccg
     7441 gaagagagtg cagttgttgt gcagggcttc ggcaacgtcg gcagcattac agcgcgtctc
     7501 ttgcaagatg aactcaaatg caaggtggtc gggctgagcg atatctccgg cggcgtctat
     7561 aacccgaggg gtatcgacat acatcgcgcc ctgcgtcatt ctaaagaaca tggcacgctg
     7621 catgggctta tcgataccga gtcggtcacg aacgcggaac tgctcgaact gccctgcgat
     7681 gttctgatcc cggctgcgct cgaaaaccag ttgacgggca ggaacgccgc gcgcgtcaag
     7741 gcgcgtctgg tgatcgaggc tgccaacggc ccgaccacgc cggacgccga tgtgatcctg
     7801 aatgatcgcg gcgtgacgat tgttcccgac attatggcca acgcgggcgg cgtgaccgtc
     7861 agctatttcg agtgggtgca ggacttgcaa cgcttcttct gggccgaaca cgagatcaac
     7921 aatcgcctgg aaagcatcat gacgcgctcg tatgacgcgg tgcgccagaa ggcagcggag
     7981 caggactgca acctgcgcat gggcgcgtac ctgctggcgg tggcgcgcgt ggccgaggcg
     8041 accgagattc gcggcgtgta tccgtaagat gatacgtgcc tgatacctcc ttgctatcta
     8101 catgttcctc gccttcgagt acagttactc ttgtagcaag atgctacacg gctctaagac
     8161 aggaagcaaa atcccgcaag gaggtggcag tatgatgcgc gcccgtccct tttctctcgt
     8221 tttgagtggc tgctgttttt tgttgctgct tgctggctgc ggctacaccc agccggcggg
     8281 aaccattagc gtcgctacct atacgccgtc cgcgaacaat ggcggtatcc ctgaagcgct
     8341 ggagtggtcg ccggatggga cgcgcctcgc gaccgcctac agcagcggct acatcgaaat
     8401 atggcaagcc acgaccggca aacgcttgct cgtctatacc ggacacaagg tgtccaaccg
     8461 cgtaggggga ctgtacacgg ttgcctggtc gccggatggc aactcgatc
//
LOCUS       DASZAK010001137         7007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_597947, whole genome shotgun sequence.
ACCESSION   DASZAK010001137 DASZAK010000000
VERSION     DASZAK010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7007
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_597947"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..616
                     /locus_tag="VGT44_20160"
     CDS             185..616
                     /locus_tag="VGT44_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583186.1"
                     /translation="MIDIYAVAGTFADPKRLAIDAATTVKTIEGVPDIPMFRKNTAAF
                     VHELPAGTLANVDGDETYVRVQVLTNEGALDREKQLAVVAELTKLVSTASGDTTLKDR
                     TWVLLIEAKEGGWGLWGHAHTNAELVAAARDEIAKLKSASS"
     gene            848..1696
                     /locus_tag="VGT44_20165"
     CDS             848..1696
                     /locus_tag="VGT44_20165"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2583187.1"
                     /translation="MQSGYDRQPAVDVEDLTITDGPSAQVALRILRPQNAHAPLPVIL
                     YIHGAGWVFGSPQTHDRLVRELAVGAGAAVVFPVYRLCPEVRYPTALEECYTAARWVG
                     RCGREHGLDAGCLAVAGDCVGGNLATVLTLLSRERGEPDIRLQVLFYPVTDAAFDTAS
                     YHQFAEGYHLRRDTMMWCWDQYTRHPSERSEITASPLRASLAHLHGLPPALIITAEAD
                     IVRDEGEAYADNLRQAGVRVAAVRFLGTIHDFVTLNALAHSAAARGAIALATAWLREG
                     FSTQLN"
     gene            1819..2541
                     /locus_tag="VGT44_20170"
     CDS             1819..2541
                     /locus_tag="VGT44_20170"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2583188.1"
                     /translation="MGIFASKRTTNKANIVLVHGFWADGSGWSKVIPALQAQGHFVIA
                     EQNGLTSLADDVTTTRQILAELDGPTLLVGHSYGGFVISEAATDVPGVVGLVYISAFI
                     LDEGETLLGSAAQFPPLDVFQHLLVSAQGLAWIDPDVFPQNFANDLDLEQARTLAVSQ
                     VPPSASIFETKAGRPAWKSLPSWSLFGSEDRMIHPDQHRWMAQRSTATTREISSSHIP
                     FLSHPDEVAHFILQAANEAVPA"
     gene            2720..3241
                     /locus_tag="VGT44_20175"
     CDS             2720..3241
                     /locus_tag="VGT44_20175"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2583189.1"
                     /translation="MSAEEHEWVHRLYQDTTHVGLKSRCHIILLSAEYYSVPQIASLL
                     FSSEDTVARCIHEFNRAGLEGILPQERVGHPAKITEEFLKKLLELVESDPRDLGYPFS
                     TWTAELLAVALQEQTGLQVSERSVGRSLHREGYGVQRPVRSVSSPDPEYEQKAVRLQE
                     LQRQAEAGEIELY"
     gene            3257..3751
                     /locus_tag="VGT44_20180"
     CDS             3257..3751
                     /locus_tag="VGT44_20180"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV2583190.1"
                     /translation="MALLPGVMRCWSKTGHQRKIDTPRQNHKQYGAGLVDWVSGKLYW
                     ATSDHKNNALFRSALSQLIEPDAGQPVRKKYVVVDNYRIHFAKPVLAFLEAHQDEIEL
                     VCLPTYSPKLNPVERFWKHLRRQVTHNHFFQTMQRLMEAVLSFFQDMAASPELVRRVA
                     GLAA"
     gene            4185..4694
                     /locus_tag="VGT44_20185"
     CDS             4185..4694
                     /locus_tag="VGT44_20185"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEV2583191.1"
                     /translation="MNAPRQVITFKPGGMRFTYRVGGVLIQQEHVLCQSASEEGFFFL
                     PGGRAEVGESARVSLLRETQEELGEALQVGRLLYVVENFFSDDRGAWHELGLYFLLSA
                     LAGSFLMHGLEPFTRQDEVGNTLCFDWLPLAHLEAFPLYPPFFQTALKDLPEHPVHIE
                     EHRSRSEHA"
     gene            5010..5447
                     /locus_tag="VGT44_20190"
     CDS             5010..5447
                     /locus_tag="VGT44_20190"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEV2583192.1"
                     /translation="MPQKAPRLSYFSVMLYHLTGERAYRGSADSPAGFLKLTTTGRKS
                     GKQRSVHLIYIRDGSAYVVTASNGGKQRPPGWLFNVRSNPQVTIQVQDRQVSAVAEVA
                     EPDKRRELWARLLSIAPFYAGYEKRTSREIPMVILQPVDEQRP"
     gene            complement(5530..5811)
                     /locus_tag="VGT44_20195"
     CDS             complement(5530..5811)
                     /locus_tag="VGT44_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583193.1"
                     /translation="MMTSDFRTQQSAFDASTAFRVASGFQAETLVSQLPRRERWVLLL
                     LDGKRSVAQVARLTRRTELDVSYTLARFLQWGYIEAVDTGIWRADGPGR"
     gene            complement(5910..6869)
                     /locus_tag="VGT44_20200"
     CDS             complement(5910..6869)
                     /locus_tag="VGT44_20200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2583194.1"
                     /translation="MIEHQALARLQDHLEFEHVLIDEMGELLPGKLLEECDLTHAQSM
                     LEIGCGGGEWLRAVARQYPDLQCIGIDQDEHLVKAANALSYRDGLSQVAFLAMDMSEL
                     SPALFPETSFDLVQLSFLGRYILTADYPGLARTCAALCRPGGVVCWIEAELPLTTSVA
                     FERLVALVCEALQRAGQSFISESMWEWTELVATRSGKPGVDRSSYQRRHLGITPMLGS
                     FQRDAGCGAQRERPIFSIWGIDERVIHQTAYVLEVSAGQPAHEGFVALARRFAHQVKQ
                     FLLRSGVIEELEHTTLCHQLDTELASQEFCGLSFLLRAWAPRP"
BASE COUNT         1498 a         2051 c         2114 g         1344 t
ORIGIN      
        1 cgcgttgcta tgtgatgcaa tgtgtggtgc gaaagtgtcg caatgtccgg tgacttcgcg
       61 tccgctgctc agtatactac aaaacagaag cctcctcgca atgaacggct tggacctgtt
      121 cagagcggtc ctgtaagtct acacatcaac tactcattca gacagagagg ggaacgctat
      181 gccaatgatc gatatctacg ccgttgctgg gacgtttgcc gatcccaagc gactggcaat
      241 tgatgcggcc actaccgtga aaacgatcga gggagtgccg gatattccga tgttccgcaa
      301 aaacaccgcc gctttcgtgc acgagttgcc cgcgggcaca ctcgccaatg tggatgggga
      361 cgaaacctat gtccgcgtgc aggtcctgac caacgagggg gccctggacc gcgaaaaaca
      421 gctcgccgtt gtagcagagc tgacaaagct cgtgagtact gcttccggtg acacaacact
      481 gaaagaccgc acgtgggtgc tcttaataga agcgaaagag gggggctggg gcctctgggg
      541 ccatgctcac accaatgcgg aattggtggc tgcggcacgc gatgaaatcg cgaagctgaa
      601 gtctgcgtca agttaacaac attatgggag agcgaaagaa caacagcgca aatttttcgg
      661 gctcaccgca aaggaacgtt cttgtgcagc agacacgctg tgcgctgtca gatgtcattg
      721 aggagccaga tccatgaccg accagattca gctggaaccc gaggcgcacg ccttcgcaga
      781 ggcggctgct cgatcacctt tgctgtacac gcttggccca gagcagggac ggctcgctct
      841 cgatgagatg caatctggct atgatcgcca gcccgcggtc gatgttgaag acctgaccat
      901 taccgatggt cccagcgcgc aggtggcgct gcgcattctg cggccccaaa acgcgcatgc
      961 cccgctgcca gtcatcctgt atatccatgg cgccgggtgg gtcttcggca gtccgcagac
     1021 gcatgaccgg ctggtgcgcg aactcgcggt cggagccggg gccgccgtcg tcttcccggt
     1081 gtaccggctc tgtccggaag tgagataccc gaccgccctc gaggagtgct acacggcggc
     1141 gcggtgggtc gggagatgcg ggcgggagca tggcctggat gccgggtgcc tcgcggtggc
     1201 cggcgattgc gtggggggca acctggccac ggtgctcacg ctcttgtccc gggagcgcgg
     1261 ggagccggac atccggctgc aggtgctgtt ctacccggtc accgatgcag ccttcgacac
     1321 ggcgtcctac caccagttcg ccgagggcta ccacctgcga cgcgacacga tgatgtggtg
     1381 ctgggatcaa tacacccgcc acccgagcga gcgcagcgag atcaccgcct ccccgctgcg
     1441 ggccagcctg gcgcacttgc acggactccc cccggcgctc atcattaccg ccgaggccga
     1501 cattgtgcgc gacgaggggg aggcctacgc tgacaatctg cggcaggccg gagtgcgcgt
     1561 agcggcggtc cggttcctgg gcaccatcca cgacttcgtg acgttgaacg ctctggcgca
     1621 cagcgcggcg gcgcggggag caatcgcgct tgcgacggcg tggctgcgcg agggcttctc
     1681 aacgcagctc aactaggcgc tgtgtgctac caccagcatc aggagaggaa cgcgcgaaca
     1741 tcgttacatg gagtgcagat atttcaggaa agcaagagca gcgttgctgc tcaaaacatg
     1801 aaaaaaagga gaacaggtat gggcattttc gcgtccaaac gcaccaccaa caaagcgaac
     1861 attgtcttag tccacgggtt ctgggccgat ggctccgggt ggagcaaagt gataccggcg
     1921 ctgcaggccc agggccattt cgtgatcgcg gagcagaacg gcctcacctc cctggctgac
     1981 gacgtaacca caacgcgtca gattctggcg gagttggatg gaccgacact gctggtcggc
     2041 cactcctatg gcggcttcgt catctccgag gcggctaccg acgtgccggg agtggtgggc
     2101 ctggtctata tctcggcctt catcctggat gagggagaaa cgctgctcgg ctccgctgca
     2161 cagttcccac ctctggacgt cttccagcat ctgctcgtta gcgcgcaggg gttggcctgg
     2221 atcgatcccg atgtcttccc ccagaacttc gccaacgacc ttgatctgga gcaggcgcgt
     2281 actctggctg tgtcccaggt gccccccagc gcgagtatct tcgagacgaa agccggacgg
     2341 ccagcctgga aatccctgcc ctcgtggtcg ctgtttggct ccgaggatcg catgatccat
     2401 cccgatcagc accgctggat ggcgcagcgc agcacggcca cgacccgcga gatctcgtcc
     2461 agccatatcc cgttcctctc gcatcccgac gaggtggcgc acttcatcct gcaagccgcg
     2521 aacgaggctg ttcccgcctg acggcgaaca taacacgcgt caggagtacc ccgtaaaatc
     2581 gatgtgccgt gtgcttgcag tttctgaaag tggctaaata aaccggcgaa ataaatgcgg
     2641 gaaatctggt aaaataaagg gtactaagac aaacgaagga ctgagtatga gcaggccaag
     2701 cgaagtgtag gtgcgctcct tgtcggcgga agaacacgaa tgggttcatc gcctctatca
     2761 agacacgacc catgtcggct tgaaaagtcg atgtcatatt atcttgctgt ctgctgagta
     2821 ctacagcgtc ccgcaaatcg cctcgctgct cttcagtagc gaagatacgg tcgcgcgctg
     2881 catccatgag ttcaaccgag cgggcttgga agggatcttg ccgcaagaac gggtgggaca
     2941 cccagccaag atcaccgagg agttcctcaa gaaattgctg gaattggtcg aaagtgatcc
     3001 gcgggacttg ggctatccct tctcgacgtg gaccgccgag ttgctggccg tggctttgca
     3061 agagcagacc ggattgcaag tcagtgaaag gagtgtgggt cgcagcttgc accgcgaggg
     3121 atacggtgtg cagcgtccgg ttcgctcggt cagcagcccc gatccagaat acgagcagaa
     3181 ggctgttcgc ttgcaggagt tgcaacgaca ggccgaagct ggtgaaatcg agctgtatta
     3241 agaggatgag gtcgacctgg ctttgctgcc cggcgtgatg cgctgttgga gcaaaacagg
     3301 ccaccaacgc aaaattgaca ccccgcgcca gaatcacaaa cagtacggcg cagggctcgt
     3361 cgattgggtg agtgggaagc tctactgggc caccagcgac cacaagaaca acgctttgtt
     3421 tcgctcggct ctcagccaac tgatcgagcc agacgctggg cagccagttc gcaaaaagta
     3481 tgtcgtggtg gataattatc ggatccactt tgccaagcct gtgctggcct ttctggaggc
     3541 ccatcaggac gagattgaac tggtgtgctt gcccacctat tcgcccaagc tcaatccggt
     3601 ggagcgcttc tggaaacact tgcgtcgtca ggtcactcat aaccatttct tccagaccat
     3661 gcaacgactc atggaggccg tcctctcctt ctttcaggat atggcggctt cccctgagct
     3721 cgttcgtcgg gtggcaggat tagccgcata aattacgccg gtttatttag tctgcacagc
     3781 gtagtatagc gaaatatgag gcaagatgga gagacaagtg ttgcagattt atcgagagaa
     3841 gggttgtgct cttgtgagac agttgtagag taaaatgcag tatactaggg cgaagaccat
     3901 cggggccatt ggagagctga cttgttcgca tcgcacgctg aatgcttcca catcctttgt
     3961 cagaaggtgc cgggtgccta ctctgccgag aggaaccgtc aagacctctg ttccggtcga
     4021 ggcgcctggt ctgtatgaaa acacggtgcg ggcgcaactg ggagcgggtg aggcgagagc
     4081 acccgacgga acttacttcc atccatcaga cctgtctcac gctctccaac tgctgacaag
     4141 agcgatgggc cgagcgagcg actaccgaag agaggttgga cctgatgaac gcacctcgac
     4201 aggtgattac cttcaaaccg ggcggaatgc gcttcaccta ccgtgtcggt ggcgtcctca
     4261 tccagcagga gcatgtgctg tgccagtcgg caagcgaaga gggcttcttt tttctgcccg
     4321 ggggacgcgc ggaggtgggg gagtccgcgc gagtctcgct gctgcgggag acacaggagg
     4381 aactgggcga ggcccttcag gtcgggcgtt tactctacgt cgtcgagaat ttttttagcg
     4441 atgaccgtgg cgcctggcat gagctaggat tgtatttcct gctgtctgct ctagccggtt
     4501 cgtttctcat gcacggcctg gagccattca cacgccagga cgaggtgggc aacaccctct
     4561 gcttcgactg gctgcccctc gcgcaccttg aggcctttcc tctctatccc ccctttttcc
     4621 aaacagccct caaggatctg ccggagcatc cggtccacat cgaagagcac cgctcccgct
     4681 ccgagcatgc ctgacttttc cggcatgtgc tgactcaagc aatgcgatcg ggagtaacca
     4741 gaacagtacc aggagggaca cgcacggtgc agattgctcg agagcaggaa agagtgcatg
     4801 tggactggga caaggaggac cgggagtcga gatgtgaaga atgaagaagg atcatcagga
     4861 gttgtggacc ctctggtgcg actgaactgg actggggcac ctgaacatgc tacactgaca
     4921 gcaaaagcgg atctcaggcc acgagccatc cctacgcatg cagaggtgca cagcctggag
     4981 acctcccacg agatgaaagg aagcgaacca tgccacaaaa agcccccaga ctgagctact
     5041 tctccgtcat gttgtaccat ctgacgggag aacgggccta ccgaggcagc gcggacagcc
     5101 cggcgggctt cctcaagctc accaccaccg gacgcaagtc tggcaaacaa cgcagcgtgc
     5161 atctgatcta tatccgcgat gggtcagcct atgtggtgac tgcctcgaac ggggggaagc
     5221 agcgccctcc tggctggttg ttcaatgtgc gcagcaatcc acaggtcacc atacaggtgc
     5281 aagacaggca ggtgagcgcc gtggcagagg tagccgaacc tgacaaacga agggaactgt
     5341 gggcacggct gctctcaatc gccccgttct acgccgggta tgagaagcgt acctcgcggg
     5401 aaattccgat ggtgatcttg cagccagtag acgagcagcg accctagctg ctcccacatc
     5461 cggtcaacgt catcttccct atggcctctg cgttgaccga aggagaaagc tgagcgaagc
     5521 agcgccatct caacgtccag gtccatctgc tcgccatatc cccgtgtcga ctgcctcaat
     5581 gtagccccat tgcaggaacc tggccagcgt ataagataca tcgagttcgg tgcgccgcgt
     5641 caaacgtgcc acctgggcaa ctgagcgctt gccatcgagc agcaggagga cccagcgttc
     5701 ccggcgcggg agctgactca caagcgtctc tgcctggaag ccggaggcaa cgcggaacgc
     5761 ggtcgaggca tcgaaagcgc tctgctgtgt gcgaaaatca gaagtcatca ttcctcaccc
     5821 tcttcgaaat ggcgctggaa cgtaccatca caacgtgctt ctttgcggtg tacgcataca
     5881 acctgtgtct gctagacgac aaagcatggt cacggacgag gtgcccaggc acgtaggagg
     5941 aaggacagtc cgcagaactc ctggctggcc agttctgtgt cgagctggtg gcacagcgtc
     6001 gtgtgttcca gttcctcaat taccccgctg cgcagcagga actgcttgac ctggtgggca
     6061 aagcgcctcg ccagagccac gaacccctcg tgagcgggct gacctgctga cacctcaagc
     6121 acatatgccg tctggtgaat cacacgctca tcgatccccc agatactgaa gatgggcctt
     6181 tcgcgctgcg cgccacagcc cgcatcgcgc tggaagctgc ccagcatggg agtgatgccc
     6241 aggtgacggc gctggtacga ggagcgatca acccctggct tgccggagcg ggtcgccaca
     6301 agctcggtcc actcccacat gctttcggaa atgaagctct gcccggcccg ctgcaacgcc
     6361 tcgcagacga gcgcgaccag ccgctcaaat gccacgctcg tcgtcagcgg cagttcggcc
     6421 tcgatccagc acaccacgcc tcctggacga cacagggcag cgcatgtcct ggcgagtccg
     6481 gggtagtccg ccgtcaggat gtagcgaccg aggaagctga gctggacgag atcgaagctc
     6541 gtctcgggaa agagcgcggg ggaaagctcg ctcatgtcca tggcaagaaa ggcgacctgt
     6601 gatagcccat ctcgatagga caaggcattg gccgctttca ccaggtgttc atcctggtcg
     6661 atgccgatac actgcaggtc ggggtattgt cgcgctaccg cacgaagcca ctccccaccc
     6721 ccgcagccga tctccagcat agactgggcg tgcgtcagat cgcactcctc cagcagtttc
     6781 ccagggagga gttcacccat ctcatctatg aggacatgct caaactccag gtggtcctgc
     6841 aggcgcgcca gtgcttggtg ctcgatcatc catttcctcc ctgcgtcgtc tgatggctcg
     6901 cggccagtgc cagcggctga tctggtaagt atgattcggt ggcatggtcc tcatcgtgag
     6961 cgactccaag atgcatctct agaagcgcga ggatggtgtg ggtgcga
//
LOCUS       DASZAK010001138         5721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_134180, whole genome shotgun sequence.
ACCESSION   DASZAK010001138 DASZAK010000000
VERSION     DASZAK010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134180"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..802)
                     /locus_tag="VGT44_20205"
     CDS             complement(<1..802)
                     /locus_tag="VGT44_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEV2583195.1"
                     /translation="MLYIGCPMWGYKEWVGNFFPPNTTQSAFLRLYSRRLTSVEGNTS
                     FYAVPDAATIARWRGETPDSFRFCFKIPREISHTPRLDTRQRELQAFLARMRPMGERL
                     GPFFLQLPPAFGPKQIEQLAAFLDYWPTGQNGLGNPRLAVEVRHPDFYQEPYTSLLDT
                     MLKQHQVARVIMDTRPIRTGSTQEQQVLQARERKPDLPLHIAATADYAFVRYIGHPRM
                     EVNDPLLDAWAERCAHWIQSGMTLYIFCHCPFEVHSPDTCRALYERVRR"
     gene            1023..1415
                     /locus_tag="VGT44_20210"
     CDS             1023..1415
                     /locus_tag="VGT44_20210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583196.1"
                     /translation="MPVTCTPDIDELVPPETETQTLRQRGGWRLRPQPRVGLDLGSDD
                     ELLMVENMTGVRWIIYHNYHRLSIIEANELLVFHIHKHGSLSARPYEDSSNAVEYLVL
                     PLNYSVTYVHIYRHSISQGIDVYDMRVA"
     gene            1533..2213
                     /locus_tag="VGT44_20215"
     CDS             1533..2213
                     /locus_tag="VGT44_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583197.1"
                     /translation="MLFSGSSYRRHRVIISGALILMALLGLFVQTGLADGALKSIRYS
                     LSAFSLSNNPQGFDISTELPPMPFSAADHVVREDSAARDQYYSDYQWHVWSYSSCSGF
                     AMEMSINAYGNHYIAADFLQKEADLGVWNVAAGLTGGEAGIAETASYFGFKALPNPPR
                     TLQALIYAVNHGYPVIVGSPSHIMVARGADSTYIYVADSSLVDRTAMTYAQFMAWWDG
                     FSVVLVPR"
     gene            complement(2210..3010)
                     /locus_tag="VGT44_20220"
     CDS             complement(2210..3010)
                     /locus_tag="VGT44_20220"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV2583198.1"
                     /translation="MLELLQQVFRQVRAYMHSDAFNRQSVYSQSNDHTTMLFDREAED
                     IIIKGLQGSRYSFEIISEERPPLLTHPNPAYRIVIDPVDGSTNVEHGIMTATLALAVL
                     PIDAPVTPEQVQWALVGELYSGIVYEASRGDGAFCNGRKCQVSKTRSYHNSLVSINMD
                     GRCRDALRALLIEGEPPAHVRRSGSSAMDLVYVANGAYDAYIDIGDILTGESFLASAS
                     IVIEAGGIVSDGQGNPLRPITSLNEHYSIIAAGSRPLHKAILERVQAR"
     gene            complement(3118..4167)
                     /locus_tag="VGT44_20225"
     CDS             complement(3118..4167)
                     /locus_tag="VGT44_20225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEV2583199.1"
                     /translation="MPELNWKTKSTPAFPNRESTPPFSLITTETIYPSSTLPPNDPTW
                     HNRLLHGDASCVLPALLPEFAGRVDLIYIDPPFMTGRDFMNGSQLAYSDKWGSSLDAY
                     LSWLYEICLRLYDLLALEGSLYVHLDWRVAHYVKVLLDELFGPNLVGDGPGFQNEIIW
                     HYQSGGRSTRHYARKHDTILLYTKSADYCFHPERIGERRGAQKRNHMRKVVDTDGRVH
                     WTIKSAGRVYSYSEDSLMTPSDVWSDINHLHQKDPERAGYATQKPAALLERILLASTE
                     EEDFILDCFCGSGVTPSVAQQLGRRWIACDQGELAIATTRARLYAQERVYPFVLQHLE
                     HVVAVHEKPSSYDMI"
     gene            complement(4315..5631)
                     /locus_tag="VGT44_20230"
     CDS             complement(4315..5631)
                     /locus_tag="VGT44_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583200.1"
                     /translation="MRAIRAHFKYGFGLVIIVISLFMAGCASQNTPQMVKRSTQTPPH
                     GLEPSTWQSIPLPRLDLELLAYVVSPVDPATIYACTSNGSSQAQNRIALWRTQDTGQH
                     WSSLHLPASPGTGCQLSLIPTQPPRLAVLVTNPYGNLRPCDNDSLYLSDDGGNSLQHV
                     PYSSIAPEGAQTVFCQVMMSSHYLYLYYSFDDRGNALQMSLLERTGDMGVTWTRIDTT
                     FGANALFLPPQVGAKDSLAVIVRHLPLAVKDEPVLWTSLDGGASWKRVGTLPDQIGTF
                     LLAPPSANTSWLTATTPFYALAEEQIPSNLYRLRVFESGNGQQWSPTPPLPVPGASTT
                     QPGLLQALAVFADGRLFAFGVNPKNGLSGSASTQRKTSAFWLWMWNPHSSRWQVLPTP
                     LNYLADESCGLCWNAQASSSSNQANNLYVYQPDTHGSFFRVLLPSS"
BASE COUNT         1277 a         1447 c         1649 g         1348 t
ORIGIN      
        1 ggcggcgcac acgttcatag agcgcgcgac aggtgtcggg ggaatgaacc tcgaatgggc
       61 agtggcagaa gatgtagagg gtcatgccgc tctgtatcca atgcgcgcat cgttccgccc
      121 aggcgtcgag cagcggatcg ttgacttcca tgcggggatg gccaatgtag cgcacgaagg
      181 cgtaatctgc ggtggcagcg atatgcaagg gaagatcagg cttgcgttcg cgcgcctgta
      241 ggacctgctg ttcctgggtc gaaccggttc gaatggggcg tgtatccatg atgacgcgag
      301 caacctggtg ttgcttgagc atagtgtcta agagcgaagt gtatggctct tgataaaagt
      361 cggggtggcg cacctcgaca gcgaggcgcg ggttgcccag gccgttctgg ccggttggcc
      421 agtagtcgag gaaggcggcg agctgctcga tctgctttgg cccgaaggcc ggtggcaatt
      481 gcaggaaaaa tggtcctagc cgttcgccca ttggtcgcat tcgcgcgagg aacgcctgta
      541 gttcgcgttg cctggtatcc aggcgcgggg tgtgactgat ttcgcggggt attttaaagc
      601 agaaacgaaa gctgtccggc gtctcgccgc gccagcgagc gatggttgcg gcatcaggca
      661 cggcgtaaaa gctagtgttg ccctcgacac tcgtcaagcg cctgctgtac aggcgcagaa
      721 atgcgctctg cgttgtgttg ggcgggaaga aatttccgac ccactcctta taaccccaca
      781 tgggacagcc aatgtagagc attgatctca ccgcctttca tcgatattgt atcatgacaa
      841 acgttgatgc aaagtgatgt ctcgcgcata ggtattctgg tgagatttat gctataattg
      901 ttctggcttt caggcgggat gtgtgatatc agtggcgagg ctcttacagt caacatcgaa
      961 gttgttgtag cgtctttagc gtaaattttg tttgttgtcc actgagaagg attgacgcat
     1021 ccatgcctgt gacttgcacc cccgatatcg acgaactcgt tccacctgaa actgaaacgc
     1081 agacgctgcg ccagcgcggc ggttggaggc tgcgcccgca accgcgcgtc gggcttgacc
     1141 tcggcagcga tgatgaactg ctcatggtgg agaatatgac cggtgtgcgc tggatcattt
     1201 atcacaacta ccatcgcctg agcatcatcg aggccaacga actgctggtc ttccacattc
     1261 ataagcacgg ttcgctgagc gcgcgcccct acgaagatag cagcaacgca gtcgagtacc
     1321 tggtactgcc actcaattac agcgtgacct atgtgcatat ctaccgacat tcgatcagcc
     1381 agggcattga cgtgtacgat atgcgcgttg cgtaaggctc tctactccta aatagtactt
     1441 tctctgctat gccttcattt ttgctctgaa aatttcctgt taatccgtta ttctcttcag
     1501 taagagtatt tggaacagga gcgaagtcgc gtatgctatt ttccggcagc agctaccggc
     1561 gtcatcgcgt catcatttcg ggcgcgttga tcttgatggc cttacttggg ctgtttgtgc
     1621 agactggcct ggctgatggg gcattaaaga gtatacgata tagtctcagc gcgttcagtc
     1681 tctcgaacaa tccccagggt tttgacatca gcaccgaact acctcctatg ccgttctcgg
     1741 cggcagatca cgttgtgcgc gaagactctg ccgcgcgcga ccagtattat tccgattacc
     1801 agtggcatgt ctggtcctac tcttcttgct cgggctttgc tatggagatg tcgattaatg
     1861 cctacggcaa tcattacatt gccgccgatt tcttgcagaa agaagctgat ctgggtgtgt
     1921 ggaatgtagc ggcagggctg accggcggcg aagcaggtat cgccgaaacg gccagctatt
     1981 ttggcttcaa agccctaccc aatccgccta gaacgctcca ggcattgatc tacgcagtca
     2041 atcatgggta tccggtgatc gtgggcagtc ccagccatat tatggtggcg cgcggagcag
     2101 atagtaccta catctacgtg gccgactcct cgctcgtcga ccgcacagcc atgacctacg
     2161 cgcaatttat ggcatggtgg gacggcttca gcgtggtgct ggttccccgc taacgcgcct
     2221 gcactcgttc aaggatggct ttgtgtaagg gcctgcttcc ggcggcgatg atggaataat
     2281 gctcattgag cgatgtaatg gggcgcagcg ggttgccctg accatcgctg acgattccac
     2341 cggcctcgat tacgatgctg gccgacgcca ggaatgactc tcccgtcaga atgtccccga
     2401 tgtcgatgta ggcatcatac gcgccattgg caacgtagac caggtccatc gcggaacttc
     2461 ccgaacggcg cacatgggct ggcggctcgc cctcgatcag cagggcgcgc agggcatcgc
     2521 gacagcgacc atccatattg atactgacca ggctgttgtg atagctgcgc gtcttgctga
     2581 cctggcattt acgcccgttg cagaacgcgc catctcctcg gctggcctca tagacgatgc
     2641 cgctgtagag ttcgccgacc agcgcccatt gaacttgctc cggcgtgaca ggcgcgtcga
     2701 tgggcaggac tgccagcgcg agcgtggcag tcatgatgcc gtgttcaacg ttggttgaac
     2761 catccaccgg gtcaatcacg atgcgataag cagggtttgg atgtgtcaag aggggagggc
     2821 gttcctcgct aatgatctcg aaactgtaac ggctcccttg tagacccttg atgatgatat
     2881 cttcggcctc gcgatcaaag agcatggttg tatggtcatt tgactgcgaa tagacgctct
     2941 ggcgattgaa ggcatcggag tgcatgtaag cgcgcacctg gcgaaaaacc tgctgtaaca
     3001 attctaacat gctggtgtaa cttcactttt ctctaaggaa atacaatcag gttggaacga
     3061 gcgtttgtgg ccgactgcgg cttgttgctg tgcttaactg ctcccttgct tcgtcgatta
     3121 tatcatatca tatgatgaag gtttttcatg tactgcgaca acgtgttcga ggtgttgcag
     3181 gacgaatgga tacacgcgct cctgagcata gaggcgcgcg cgcgtggtgg caatggcgag
     3241 ttccccctgg tcgcaggcga tccagcggcg gccaagttgc tgcgctacgg agggtgtgac
     3301 gccgctgccg caaaagcaat cgagaataaa atcctcctct tccgttgagg ccagtaagat
     3361 gcgctcgagt agcgctgccg gtttctgcgt ggcgtaaccg gcgcgttcgg ggtctttctg
     3421 gtgcaggtgg ttgatgtcgc tccagacatc cgatggcgtc atgagcgagt cctcgctata
     3481 ggagtaaacg cgtcccgctg acttgatcgt ccaatgcact cgaccatctg tatccaccac
     3541 tttgcgcatg tggttgcgtt tctgcgcgcc cctgcgctcg ccgatgcgtt ccgggtgaaa
     3601 gcaatagtcg gcggactttg tatagagcaa gatcgtatcg tgcttgcgcg cgtaatgcct
     3661 ggtcgagcga ccgccagatt gataatgcca gataatctcg ttttgaaagc cagggccgtc
     3721 cccgaccagg ttagggccga acagctcatc cagcagcact ttgacataat gcgcgacacg
     3781 ccagtccagg tggacataca ggcttccctc aagcgccaac aaatcataga gcctcaagca
     3841 tatttcgtat agccacgata aatacgcatc gagactgctg ccccacttat cgctataagc
     3901 cagttgagag ccattcatga aatcgcgccc ggtcataaat ggcggatcaa tatagatcag
     3961 atcgaccctg ccagcgaatt cgggaagcag agcgggcagc acacacgaag cgtcgccatg
     4021 aaggaggcga ttgtgccagg tcggatcgtt gggagggagt gtggaagagg gatagatggt
     4081 ctcggtcgta ataagtgaaa aaggtggggt actttctcga tttggaaaag ccggagtcga
     4141 tttcgttttc cagttgagtt cgggcatgtg agttcgctct acttttttaa ttcagattgt
     4201 atcaccagca tcatatgcaa gaagcggctg agtgtcaaac attgagacta ttgcccagga
     4261 atactcactg atctcacctg gtgccagatt attttgttaa cctccatcaa cgaatcaaga
     4321 agatgggagg agtacacgaa agaagcttcc atgagtatcc ggctgataga catagagatt
     4381 gttggcttga tttgagctac ttgaggcttg cgcattccag caaagcccac aactttcgtc
     4441 ggcaagataa ttcaatggtg tggggagaac ctgccagcga gatgaatgag gattccacat
     4501 ccagagccag aaagcggacg tttttcgttg ggtgctggct gagccggata acccattttt
     4561 cgggttaacg ccaaaggcaa acaggcggcc atcggcgaag actgccagcg cctgtaacag
     4621 gccaggttgc gtcgtacttg ctcctggtac cggcaatgga ggagtaggag accattgctg
     4681 cccgtttcca ctctcaaata cacgtaatcg ataaagattt gatgggattt gttcctctgc
     4741 cagagcgtag aatggagttg ttgccgtcaa ccaggaagta tttgccgagg gtggcgcgag
     4801 caagaatgtg ccaatctggt caggaagagt tcctacgcgc ttccaggagg cgccaccatc
     4861 aagactcgtc cacagtaccg gctcgtcttt cacagcgagc ggtaagtgcc gcacgattac
     4921 agctagcgaa tctttagcgc cgacctgcgg aggcaagaag agcgcattgg cgccgaatgt
     4981 tgtatcaata cgtgtccagg tgactcccat gtcgccagtg cgttccagca agctcatctg
     5041 cagtgcgttt cctctatcat cgaatgagta atagaggtag aggtaatgac tactcatcat
     5101 gacctggcaa aagacggttt gtgcgccttc aggagcaatg gagctatagg gaacatgctg
     5161 caggctattg cccccatcat cgctcaagta caggctatcg ttgtcacagg ggcgcaaatt
     5221 tccgtaggga ttggtcacta aaacagcgag gcgcggcggt tgagtgggga tgagcgatag
     5281 ttgacatccg gtgccgggac tggcgggaag atggagcgat gaccagtgct gaccagtatc
     5341 ctgcgtgcgc cagagagcta tgcgattttg cgcttgcgag cttccattgc tggtgcaggc
     5401 atagattgtc gcgggatcga ccggcgaaac gacgtatgcg aggagttcga gatctaatct
     5461 gggcaaaggg atcgactgcc acgtagacgg ctcaagccca tgtgggggag tttgtgttga
     5521 ccgcttgacc atttgtggcg tgttctgaga agcgcaacca gccataaaca gggaaataac
     5581 aatgataacc aggccaaatc cgtatttgaa atgggctcta atcgctctca taagatgaac
     5641 tccttccaac ctgtttggtg cattgagatt ggttagagaa gaaaattatg taccctgatc
     5701 tgtattatac agtacctcat g
//
LOCUS       DASZAK010001139         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_29278, whole genome shotgun sequence.
ACCESSION   DASZAK010001139 DASZAK010000000
VERSION     DASZAK010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29278"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VGT44_20235"
     CDS             <1..657
                     /locus_tag="VGT44_20235"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEV2583201.1"
                     /translation="GSGRIDRVVAVVNREPILQSDIEDEMQLSVLDPSTNANEQMTQK
                     AALERLISRALIQQQMQREDLEATEPKAGDVAARIEEIRTEVPACVRADCRSDAGWKT
                     FLAHHELTPAQVEIYIRHRMEILNFIELRFQQGIRIAPDEIETYYSGTLLPQYPAGQK
                     PPPLEQVSSRIEEILLQQRVNVLFDSWLSNLRKQGQIEVLDPALETADAANDSGATKE
                     "
     gene            654..>2069
                     /locus_tag="VGT44_20240"
     CDS             654..>2069
                     /locus_tag="VGT44_20240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583202.1"
                     /translation="MSETSKIPAAPPDSPDENQKPQLAWHRRYLRHVLRVGAAIVLLL
                     VVGLVGLYFWASSSSFENIMRRRLIARIDAAMGGRTEIASFHWNLLKLQVEADGFVLH
                     GREAPGEAPYARMDSLKVDVDVLGFFSPRILLRELVLTHPQVHLIVYADGSTNQPQPV
                     QKTETHPLDTLFDLQAGHVEVNHGAIQYDDRADANDFQNRRIPVDFEANDVSLLLKYV
                     AGNGVKPESYHLDAGIRDIRLVRGTAAHPDAPPVEGFAEASVDFTNNAVYLRSLVLTA
                     HSKGSADRVLRIAGELDDFTHPRWNASVQGELDLKLMEPALGYPSTPEGIAKLNLVAA
                     GHAGEFRIDGTVHADNASYIGTGVVARGVRLDAHVHADALRLQITNVTARLKAGGQLE
                     GEVLLDHWIPPLSGAPVVQAAEPPKREKKGRIHEPATPPATAKVDTNLHTDGKVNAYF
                     RDVTLDTLLDMVSQPPFQRLGM"
BASE COUNT          410 a          639 c          647 g          373 t
ORIGIN      
        1 gggtcgggcc gcatcgatcg cgtggtcgcc gtggtgaacc gggagccgat tctccaaagc
       61 gatattgaag acgagatgca gctgtcggtg ctcgacccga gcaccaacgc gaatgagcag
      121 atgacgcaga aggcggcgct cgagcggctt atcagccgtg ccctgatcca gcaacagatg
      181 cagcgcgaag acctggaggc gactgagccg aaggccggcg atgtcgccgc gcgtatcgaa
      241 gaaattcgca ctgaggtccc ggcgtgcgtt cgcgcggatt gccggtcgga cgcgggatgg
      301 aagactttcc tcgcgcacca tgagctgaca ccggcccagg tcgagatcta catccggcac
      361 cgaatggaga ttctcaactt tatcgagctg cgattccagc agggcattcg catcgcgccc
      421 gacgagattg agacctacta ctcagggacg ctgctgccgc aatatccggc cggccagaag
      481 ccgccgcctc ttgagcaggt gtcgtcgcgc atcgaggaga ttctgctcca gcagcgcgtc
      541 aacgtgctgt tcgacagctg gctctccaat ctccgcaaac aaggtcagat tgaagtactg
      601 gacccggcgc ttgaaacggc ggatgccgct aacgattcgg gagcgaccaa ggaatgagcg
      661 agaccagcaa gattcccgcg gctcctcctg attctcctga cgagaaccag aagccgcagc
      721 tcgcctggca tcggcgatat ctgcgccacg tgctccgggt gggggccgcg attgtacttc
      781 tgctcgtcgt cggtctggtg gggctctact tctgggccag ttcctcgtcg tttgaaaaca
      841 tcatgcgccg gaggctgatt gcgcggattg atgcggcgat gggcgggcgc acggagattg
      901 cctccttcca ctggaatttg ctcaagctcc aggtcgaggc cgacgggttt gtcctgcatg
      961 gccgcgaagc gccgggcgag gcgccgtacg cccgcatgga ttcgctcaag gtcgacgtcg
     1021 acgtgctggg cttcttcagc ccgcgaattc tgctccggga actcgttctc acgcatcccc
     1081 aggttcacct gatcgtctac gccgacggat ccaccaatca gccacagccg gtccagaaaa
     1141 cagagaccca tccgctggac accctgtttg atctccaggc cggccacgtc gaggtgaatc
     1201 acggcgcaat tcaatacgac gatcgagccg atgccaacga ctttcagaac cggcgtatcc
     1261 cggtggactt cgaagccaac gatgtttccc tccttctgaa atacgtcgca ggaaatggcg
     1321 tcaagccgga gagctatcac ctggacgccg gcattcgcga catacgcctg gtgcgcggaa
     1381 cagccgcaca tcccgacgcg ccgcccgtgg aagggttcgc cgaggcctcg gtcgacttta
     1441 caaacaatgc cgtgtacctg cgttccctgg tgttgacagc gcacagcaag ggatccgcgg
     1501 atcgcgtact gcgcatagcc ggcgagctcg atgacttcac tcatccgcgc tggaacgcct
     1561 cggtgcaggg ggagcttgac ctcaagctca tggagccggc tctgggttat ccgtctacgc
     1621 cggagggtat tgcaaagttg aacctcgtgg cggcaggcca cgcaggcgag ttccgcattg
     1681 atggaacggt gcatgccgac aacgcttcct acatcggaac gggcgtggtg gcgcgaggcg
     1741 tgcgactgga cgcgcatgtg cacgccgatg cgttgcgcct gcagattacc aatgtgacgg
     1801 cgcgcctcaa ggccggcgga cagcttgagg gcgaggtgct gctggatcat tggataccgc
     1861 ctctttccgg tgcgccggta gtgcaggcgg ccgagcctcc gaagcgtgag aagaagggcc
     1921 ggatccacga gccggccacg ccgcccgcaa ccgcaaaggt tgacaccaac ctgcataccg
     1981 acggcaaggt gaacgcctat ttcagagacg tcacgctgga tacgctgctc gacatggtaa
     2041 gccagcctcc cttccagcgc ctgggaatg
//
LOCUS       DASZAK010001140         6447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_385669, whole genome shotgun sequence.
ACCESSION   DASZAK010001140 DASZAK010000000
VERSION     DASZAK010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385669"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="VGT44_20245"
     CDS             <1..1098
                     /locus_tag="VGT44_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S53"
                     /protein_id="HEV2583203.1"
                     /translation="NYSGTAANSGSDVIGMTKLGPAFYFGDMAVGAQSGGSFASFLTI
                     LNPPASPTATVTATYYAGGIQVGQQVVIVAGGTRGTIYPNRASQALPARVAVVVTSNQ
                     PVAVERPTYFDHIAAGNAGTISGAADVIGVQTLSNDWLFAEGYTGGKFQENLVIANVD
                     LAHAAAAVTVTLEFPNGTTQTSNFNVPANSQYVYNVNTLGGGQSVSAEVKSSGAQIVA
                     EREMFFQYSHNANGRILNAVGGTDVLGQSGPAALSNYSFAEGYVNVGYDEWLTIQNPT
                     AVTEAITVGLVNAKGTVYIFQLSVVGHSRYTVDITGTVIQHLYHTGDGFMGYEVAMVV
                     QSSSPFIAERPMYWNTSGTTGGSATIGYVGG"
     gene            complement(1108..4350)
                     /gene="uvrA"
                     /locus_tag="VGT44_20250"
     CDS             complement(1108..4350)
                     /gene="uvrA"
                     /locus_tag="VGT44_20250"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV2583204.1"
                     /translation="MPQDKIVIRGAREHNLKNIDVTIPRDQLVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFLDKMEKPDVDHIDGLSPAISIDQKGATHNPRSTVGT
                     VTEIYDYLRLLYARVGHPHCPNCGREVSRQTVQQIVDAVMSLPEGSRIQLLAPLVQGR
                     KGEYKQVFEEMRRAGYVRVRVDGTIYDLSDEIDLDKQKKHTIEVVVDRLVIRTRARNV
                     YDESRQPRALRKVAERPALYDVNAQRDVVDGTDGAVANDGAHPIELRAVQVDEMPAAE
                     DLDLSFRQRVTDSLETTLKLGNGVVLVSIIDGEEILYSEKLSCVYCGISLPEIEPRTF
                     SFNSPHGACPTCTGLGTQQEIDAELVVTHPELSLNQGAIAPWSKVVNGSLWHSAILEA
                     LAKKYSFSLDTPWRELSADHQKKVLYGSSEALTIRYTPQQGGSRSYTIHFEGVIPNLD
                     RRYKQTDSESIREEIEVYMSARICPACHGARLKPEVLAVTVGGHNIVQVSRLAIVHAQ
                     RFFMELESEQAEITGQAGSGNVRLLNGKNGNGNGKKRKDGEESLLGDPLTPIDPNGPM
                     VDVTLTERERFIGRQVLKEIRARLQFLVDVGLDYLTLDRAAASLSGGEAQRIRLATQI
                     GSGLMGVLYILDEPSIGLHQRDNARLIKTLVRLRNLGNTLLVVEHDEDTMRAADYIID
                     IGPGAGEHGGYVVGEGTYKDIIASPNSITGDYLARRKLIPIPARRREGNGKSLLIKGA
                     RENNLKHIDVQIPLGEFVVVTGVSGSGKSTLITDILYRKLAHVLHRAHDKPGAHGAVE
                     GIEHLDKIIDIDQSPIGRTPRSNPATYTNAFTGIRELFAQVPESRIRGYQPGRFSFNV
                     KGGRCEACKGEGIVKIEMNFLPDVYVPCEICKGKRYNREALEIHYKGKSIADVLDMTI
                     EEASEFFANVPSIYNKMKTLNEVGLGYMRLGQPATTLSGGEAQRVKLATELSRRATGR
                     TMYILDEPTTGLHFADVDRLLHVLQRLVDAGNSIVVIEHNLEVIKSADWIIDLGPEGG
                     DAGGYVLATGTPEDVAEQEQSFTGYYLKRLFTEEAALANVRHASETLPVE"
     gene            4637..5386
                     /gene="lepB"
                     /locus_tag="VGT44_20255"
     CDS             4637..5386
                     /gene="lepB"
                     /locus_tag="VGT44_20255"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV2583205.1"
                     /translation="MNPELDFEKRFRLVREVIETLVLTLLMFLVIRFAVQNFNVEGPS
                     MEPTLHDGELILVDKWSYLFRAPTRGDVIVFVAPPHPDQDYVKRIIGLPGDRITVDDT
                     RVYVDGVLLNETYVSQVDQGNPFNYKTINNVVIPAGMYFVLGDDRIRSSDSRDWGLVP
                     RSNIIGRAALIYWPLHQDNDGFLANVSNVYAAVHQPGSSSSMAPTNSLIFAGTNPFVM
                     LAMPGALLVFSRKRKKRVLSPKRRQSADRQQ"
     gene            complement(5416..6252)
                     /locus_tag="VGT44_20260"
     CDS             complement(5416..6252)
                     /locus_tag="VGT44_20260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2583206.1"
                     /translation="MNCSRCQAPLEEGARFCRNCGLPVPAADYAAPVASNPASGQPLS
                     QPYAPTQPFTPASPAMPAAPLPYTQPVGGAAPVNQQFGQNQPGQFVQQAPAGPVKRKR
                     HRLRGTIITLLVLIVLVVGGWFVVARPILHNYAQGQIDQVVTSNINSIVPVPINSLVA
                     TEQTIDNLIVLNSSSGPVTNAAVHISPPVFASDGTYTGGMQLDFNIFGFPCSVIAIPK
                     ASNGFIVVTHLQVNGVIAWVMSAQELTDDLNSHLHAVNGRLLRSISSVTLKTGEIDVT
                     LA"
BASE COUNT         1314 a         1933 c         1857 g         1343 t
ORIGIN      
        1 aactacagcg ggaccgcagc caatagcggc agcgatgtca tcggcatgac gaagctggga
       61 ccggccttct acttcggtga tatggccgtt ggcgcgcagt cgggcggcag ctttgcttcc
      121 ttcctcacca tcctgaaccc acccgccagc ccaaccgcca ccgtcacggc tacctactac
      181 gccggtggca tacaagtcgg ccagcaggta gttatagtag cagggggaac gcgcggcacc
      241 atctacccca accgggccag ccaggcgctg cccgcgcgcg tggccgtcgt cgtcacctcg
      301 aaccagcccg tcgccgttga gcggcctacc tacttcgacc acatcgcagc gggcaatgct
      361 gggaccatct ccggcgcggc tgacgtgatc ggcgtccaga cgctgtcgaa cgactggctc
      421 ttcgccgaag gctataccgg cggcaagttc caggaaaatc tcgtgattgc caatgtggac
      481 ctcgcccatg cagcggcggc ggtcaccgtc accctggaat tcccgaacgg cacaacgcag
      541 acgtcgaact tcaatgtccc ggctaatagc cagtatgtct ataacgtcaa tacgctagga
      601 ggcggccagt cggtctcggc tgaggtcaag tccagcggcg cgcagattgt ggcggaacgt
      661 gaaatgttct tccagtacag ccacaacgcc aatggacgca tcctcaacgc ggttggcggc
      721 accgatgtgc tgggccagag cggtcctgcc gctttgagca actacagctt tgccgagggc
      781 tacgtcaacg tgggctacga tgagtggctg acgatccaga acccgacggc cgttaccgag
      841 gctatcaccg ttggcctcgt caacgccaaa ggcaccgtgt acatcttcca actgagcgtc
      901 gtcggccatt cgcgctacac cgtcgatatc accggcacgg tgatacagca cctgtaccat
      961 actggcgacg gctttatggg ctacgaagtg gcaatggtgg tgcagtcatc gagtcctttc
     1021 atcgcggaac gccctatgta ttggaataca tctggcacaa cgggcggcag tgccaccatc
     1081 ggctacgtcg ggggctagac ggttaaatta ctccaccggc agcgtctcgc tggcgtggcg
     1141 cacattggcg agggcggctt cctcggtgaa gagtcgcttt aagtagtagc cggtgaagga
     1201 ctgctcctgc tcggccacgt cttccggtgt gccggtggcg agaacatagc caccggcatc
     1261 gccgccctcc gggccgagat cgatgatcca gtcggcgctt ttgatgactt ccagattatg
     1321 ctcgatcacc acaatcgagt tgcccgcgtc gacgaggcgc tgcaggacgt gcagcaggcg
     1381 atcaacgtcg gcgaagtgca ggccggtggt cggctcgtca aggatataca tcgtgcggcc
     1441 cgtggcgcgg cgcgacagct cggtggccag tttgacgcgc tgcgcctcgc cgccggacag
     1501 cgtcgtagcc ggctgaccca ggcgcatgta gcccaggccg acctcgttga gcgttttcat
     1561 tttgttgtag atcgacggca cattggcgaa aaactcagag gcttcctcga tggtcatatc
     1621 cagcacatcg gcgatacttt tgcctttgta gtggatctct aacgcctcgc gattgtagcg
     1681 cttgcctttg cagatctcgc acggcacgta cacatccggc aggaagttca tctcgatctt
     1741 gacgatgccc tcgcccttac aggcttcgca ccgcccgcct ttgacgttga aggagaagcg
     1801 ccccggctgg tagccacgga tgcgggactc cggtacctgc gcgaagagtt cgcgaatgcc
     1861 ggtgaaggcg ttggtgtacg tcgcgggatt cgagcgcggt gtgcgcccga tgggcgactg
     1921 gtcgatgtcg atgatcttgt ccaggtgttc gatgccctcg acggccccgt gcgctcctgg
     1981 cttgtcgtgg gcgcggtgca gcacgtgcgc cagcttgcgg tacagaatat cggtaatcag
     2041 cgtactcttg ccggagccgg agacacccgt caccaccacg aactcgccca ggggaatctg
     2101 cacatcgatg tgcttcaggt tattctcacg cgcgcccttg atgagcagcg acttgccatt
     2161 gccttcgcgc cgcctcgccg ggatggggat gagcttgcgc cgcgcgagat agtcgcccgt
     2221 gatcgaattg ggcgaggcga tgatatcttt gtaggtccct tccccgacga catagccgcc
     2281 gtgttcgccc gcgccggggc cgatgtcgat gatatagtca gcagcgcgca tggtatcctc
     2341 gtcgtgctca accaccagca acgtgttgcc caggttgcgc aggcgcacca gcgtcttgat
     2401 aagacgcgcg ttgtcacgct ggtgcaggcc gatactcggc tcatccagga tgtagaggac
     2461 gcccatcagg ccagagccga tctgcgtcgc caggcgaatg cgctgcgcct cccctccgga
     2521 aagactggcg gcggcgcggt ccagcgtcag gtaatccagg ccgacatcca ccaggaattg
     2581 caggcgcgcg cgaatctctt tgaggacttg ccgcccgatg aagcgttcgc gctccgtcag
     2641 cgtcacgtcc accatcggcc cgttgggatc gatcggcgtg agcggatcac ccagtagaga
     2701 ctcttcgcca tctttgcgct tcttgccatt tccattgcca tttttgccgt tcaacaggcg
     2761 cacatttcca gaaccggctt gccccgtaat ctcagcctgc tcggactcca gttccatgaa
     2821 gaagcgctgc gcatgtacaa ttgcgagccg cgagacctgt acgatattgt gaccgccgac
     2881 ggtgacggcc aggacttccg gcttaagacg cgcgccatga catgcgggac aaatacgcgc
     2941 cgacatatac acttcgatct cttcgcgaat gctctcactg tcggtctgct tgtagcgccg
     3001 gtccaggttg gggatgacgc cctcgaagtg gatggtgtag ctgcgcgagc cgccctgctg
     3061 cggcgtataa cggatcgtca gcgcctcgga tgacccgtag agcacctttt tctggtgatc
     3121 cgcgctcagt tcgcgccagg gcgtatccag gctaaagctg tatttcttcg ccagcgcttc
     3181 caggatggcc gaatgccaca ggctgccgtt gacgaccttg ctccagggcg caatagctcc
     3241 ctggttcagg ctcagctcgg gatgcgtcac gaccagttcc gcgtcgatct cctgctgcgt
     3301 tcccaggccg gtgcaggtgg gacacgcgcc atgcgggctg ttgaagctga aggtacgcgg
     3361 ctcgatctct ggcaagctga tgccacagta gacgcaggac agcttctccg agtagaggat
     3421 ctcttctccg tcgataatcg acaccagcac cacgccgttg cccagcttca gcgtcgtctc
     3481 cagcgaatcg gtgacgcgct ggcgaaatga caggtccagg tcttctgcgg cgggcatctc
     3541 atcgacctgg accgcgcgca gttcaatcgg atgcgcgcca tcattggcga ccgctccatc
     3601 tgtaccatcc accacgtctc tctgcgcgtt cacgtcgtag agcgcggggc gttcggcaac
     3661 tttgcgcagc gcgcgcggct ggcgcgactc atcgtagaca tttctcgctc tcgtgcggat
     3721 caccagccgg tcaacgacaa cttcgatggt atgcttcttt tgcttatcca ggtcgatctc
     3781 atcgctaaga tcgtaaatcg tgccatcgac acgcacacgc acatagcccg cgcggcgcat
     3841 ctcctcgaag acctgcttgt attcgccctt gcgcccctgc acgagcggcg cgagcaactg
     3901 gatgcgcgat ccctccggca ggctcatcac agcgtccaca atctgctgga cggtctgccg
     3961 gctcacctca cgcccgcaat tggggcaatg cggatggccg acgcgcgcgt agagcaggcg
     4021 caggtagtcg tagatttcgg tgacggtgcc gaccgtcgat cgcgggttat gcgtcgcgcc
     4081 tttctggtcg atagagatag ccggtgacag cccatcgata tgatcgacgt caggcttctc
     4141 catcttatcg agaaattgcc gcgcgtaggc cgagagcgac tcgacatagc gacgctgccc
     4201 ctcggcaaaa atggtatcaa aggccaggct gcttttgccc gaaccggaga ggccggtgat
     4261 gaccaccagt tgatcgcgcg gaatagtgac atcgatattt ttcaggttat gttcgcgagc
     4321 gccacgaatt acaattttgt cctggggcat acaactccac ccaattagaa acaaacgttc
     4381 aacagcagga ttataccata ctatacatgt cagatagcgt caagtatttc ggcgagtaat
     4441 ctaattcttc catacaatac aacaaaaaaa tagtagccct tatttcctac aaagatagca
     4501 acgcaaatgc cgatagcatt acactacagg ggaagcgaac cattacaaca gcatgaaacg
     4561 tcaatacgta taatggaata cagggatgat acgatacaat gccgcaccaa acgtccattg
     4621 tgcctgggga gtctgaatga atccagagtt agattttgag aaacgctttc gcctcgtgcg
     4681 cgaggtgatt gagacgcttg tcctgacctt gttgatgttc ctggtgatcc gctttgccgt
     4741 acaaaacttc aatgttgagg ggccaagcat ggaaccaacc ctgcacgatg gcgaactgat
     4801 cctggtggat aaatggtcgt acctgttccg cgccccgacg cgcggtgatg tgatcgtctt
     4861 cgtcgctcct ccgcaccccg accaggacta cgtcaagcgc atcatcggtc tccccggcga
     4921 ccgcatcaca gttgacgata caagggtcta cgttgacggc gtgctgttga acgaaacata
     4981 tgttagccag gtcgaccagg gaaacccctt taactataaa acgatcaaca acgtggtgat
     5041 cccggcgggc atgtatttcg tgctgggcga tgatcgcatt cgcagctccg attcgcgcga
     5101 ctggggcctg gtgccgcgct caaacatcat cgggcgggca gcgctcatct actggccgct
     5161 ccatcaggat aacgacggct tcctggccaa cgtctccaac gtctatgccg ctgttcacca
     5221 gcccggtagt tcctcctcca tggccccaac aaactcgctc atcttcgccg gaactaaccc
     5281 ctttgtgatg ctggcgatgc ccggcgcgct gctcgtcttt tcacggaaga gaaagaaaag
     5341 ggttctctcc cccaaacgga ggcagagcgc tgacaggcag cagtagaatc cacgtactcg
     5401 tttgaaaacg ctactctaag ccagcgttac atcaatctcc ccggttttca gcgtgacgct
     5461 agatatggag cgcagcaggc gaccattgac cgcgtgtaga tggctgttga ggtcatcggt
     5521 caactcctgg gcagacatga cccaggcgat gacgccgttg acctgcaggt gcgtcacgac
     5581 aatgaagccg ttgctggcct tgggaatggc tatgaccgag caggggaagc caaagatgtt
     5641 gaagtcgagc tgcatcccgc cagtataggt accatcgctg gcgaagacgg gcggcgaaat
     5701 gtggacagcc gcattggtca ccgggcctga agaagaattc agcacgatca aattatcgat
     5761 cgtttgttct gtggctacca gcgaattgat cggtaccggc acaatcgaat tgatgttgct
     5821 cgtgacgacc tggtctatct ggccttgcgc gtagttatgc aggattggac gcgctaccac
     5881 aaaccagccc cccacaacca gcacgattaa gacaagcagg gtaatgatgg ttcctcttag
     5941 ccggtgccgt ttgcgtttga cgggaccagc cggtgcctgt tgtacgaact gtcccggctg
     6001 gttctgtccg aactgctggt ttaccggagc agcgccccct accggctggg tatacggcag
     6061 cggcgcagca ggcatagctg gggaagcagg cgtgaagggt tgtgttggcg catatggttg
     6121 cgaaagcggc tgtccagagg cgggattgga cgccaccggc gcggcataat cagcggcagg
     6181 cacgggcaga ccgcagttgc ggcaaaagcg cgcgccctcc tccagcgggg cttgacagcg
     6241 agagcaattc ataaatgtat gcgcctttca aagtattgaa gttcaccggg ataatcaaag
     6301 tatacgcttt tctaaaagcc tcgtaaatga aatcgctcac atttttaggc tttgtgacgt
     6361 tcgcgcagca gaacctggtc catcgattgc atgattcgct ggcgcatttc cgcatcatgt
     6421 tcaatcgcct gcgccacctg ctcgatc
//
LOCUS       DASZAK010001141         2446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_598017, whole genome shotgun sequence.
ACCESSION   DASZAK010001141 DASZAK010000000
VERSION     DASZAK010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598017"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VGT44_20265"
     CDS             complement(<1..812)
                     /locus_tag="VGT44_20265"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2583207.1"
                     /translation="MTPTSMPANQPYLNKELFRPSLQYPSHPYDEMLTRTAQRFPENE
                     AVLFKDANLSYRELDALVNAFANALLDVGISKGQTVCLFMTNRPEYLVCWFAIARIGA
                     IVSPMNPSYREREVAYQLSNSEAVAIVVQQELLPLVEVVRAETPMLEHVITVGSGQQV
                     APTYVHSFSQMVRTHAPTLRAGSGSGWEDVLALPYSSGTTGLPKGVMLSQKNLVVNAY
                     QSVATARITFQDRMLVFVPLYHIYGIMLMGLAAMTGATMVLMARFDPGECLRL"
     gene            complement(835..2358)
                     /locus_tag="VGT44_20270"
     CDS             complement(835..2358)
                     /locus_tag="VGT44_20270"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV2583208.1"
                     /translation="MILGLPTWLQRLMDWNITRLHSRDDWMRRGRSYARRGLSEHGKV
                     DVVLGTLTMTRGRAEEVDFSDTYFVASQALLVTSASAAHATQDLDGTSVAVVKQSTAP
                     EALRTHLPRAHALLVGNFTEALQALDSGKAMALLADDAILQGLMKQHPGRYRLLATLP
                     TKEYYAAAVAKDHPELLAAVNRAVRNFNSSEAWSSSIARHLPDLSVAGPPQLALETTL
                     ADISGWKSTRSPVDGTVSAQNAGRATLLDRIKARGYLVVAVKENVPGFGYRDPQTGQL
                     QGLEIDLARAIAQEIFGDPKKVEFRPAQTSERLPLVRSFLKFFDAPVKLFSIISTALD
                     SNWWHLGMAGKLPEFLCPRECVGQQDFVGIDYYWGISTFGLHRIQELMSAMHGYYDQA
                     PVWPGELYKTLRYHAKLFPGKEILIVENGCVDEADGYDRARYIERHVQEVQRAYQSGV
                     KVVGYDCWSITSNREWGLPFNRGSDFGLYHIDLDDDPDLKRVPTPAVGVYKRIIEQP"
BASE COUNT          535 a          723 c          650 g          538 t
ORIGIN      
        1 agccgcaaac actcacctgg atcgaagcgc gccatcagca ccatagtagc gcctgtcatt
       61 gcagcgagcc ccatcagcat aataccatag atgtgataga gcggcacgaa gaccagcatg
      121 cgatcctgaa acgtgatgcg agcggtagca accgactggt atgcattgac cacgagattc
      181 ttttgactga gcatgacgcc cttcggcaaa ccagtcgtgc cactactgta cgggagcgcc
      241 agcacatctt cccaccctga tcctgagcct gcacgcaacg ttggcgcatg ggttcgcacc
      301 atctggctaa aggaatgcac ataggtgggg gcgacttgct ggcctgagcc aaccgtaatg
      361 acatgttcca acattggagt ttctgcgcgt actacctcta ccagcggcag taactcctgc
      421 tgaaccacga ttgctaccgc ctcgctattg ctcaactggt aggccacttc gcgctcccta
      481 tatgatggat tcatagggct aacaattgct ccaatgcgcg caatggcgaa ccagcacacc
      541 aggtattcag gccggttcgt catgaaaagg cacactgttt gccccttgct tatgcccacg
      601 tccagcaagg cgttggcaaa ggcgttcacc agcgcgtcca attcccggta tgacaggttc
      661 gcatccttga acagaacagc ctcgttctcc gggaagcgct gcgccgtgcg agtcagcatt
      721 tcatcatagg gatgagaggg atactgaaga gatgggcgaa aaagctcctt gttcaaatac
      781 ggttgattag ctggcattga agtaggggtc atgtctcttc actccttgtg ttgttcaggg
      841 ttgctcgata atccgcttgt agacccccac agcaggcgtt ggcacgcgct tgagatcagg
      901 gtcgtcatcc aggtcgatgt gatatagccc gaagtcgctg ccccggttaa atggtagacc
      961 ccactcacga ttggaagtaa tgctccagca gtcataccca accactttca cgccgctctg
     1021 ataggcgcgc tgcacctcct ggacgtgtct ctcgatatac cttgcccggt catacccatc
     1081 cgcttcatct acacagccat tctcaacgat caggatctcc ttccctggaa acagcttcgc
     1141 gtgatacctg agcgtcttgt acaattcacc cggccagacc ggcgcctggt cgtaatatcc
     1201 atgcatggcc gacatcaact cctggatgcg atgcaggcca aacgtgctaa tgccccagta
     1261 atagtcgatg cctacaaagt cctgctggcc aacgcactct cgcgggcaga gaaactccgg
     1321 cagcttcccc gccatgccaa ggtgccacca gttgctgtcc agggcagtcg aaatgatact
     1381 gaacagcttg acaggcgcgt cgaagaactt cagaaacgag cgtaccagcg gtaggcgttc
     1441 gcttgtctgg gccgggcgaa actcgacctt cttcgggtca ccgaaaatct cctgcgcgat
     1501 agcccgtgcc aggtcaatct ccagcccttg caattggcca gtctgtggat cgcgatagcc
     1561 aaaaccaggc acattttctt tcactgccac cacaagatag ccacgagcct taatgcgatc
     1621 cagcagcgtc gcgcgcccag cattctgagc gctgactgtt ccatctactg gactgcgcgt
     1681 cgacttccac ccgctgatgt cggcgagtgt tgtctccagt gcaagttgag gaggccctgc
     1741 aactgaaaga tcgggaaggt gtctggcgat gcttgatgac caggcttctg agctgttgaa
     1801 gtttctcacc gccctgttaa cggccgctaa cagttccgga tggtctttcg ctacggcagc
     1861 agcatagtat tctttagtag gaagtgtagc gagcaagcgg tagcgccctg gatgttgctt
     1921 catcagtcct tgaaggatgg catcgtcggc cagaagtgcc atcgctttcc cgctatcaag
     1981 agcctgcagc gcttcagtga aatttcctac cagcagcgcg tgggccctgg ggagatgggt
     2041 gcggagcgct tccggcgctg tagactgttt gacgaccgct acgcttgtgc catcaaggtc
     2101 ctgggtcgca tgagctgcgc tcgcggaggt aacgagcagc gcctggcttg cgacaaaata
     2161 ggtatcggag aaatcgactt cttcagccct gccgcgcgtc atcgtgaggg tgccgaggac
     2221 gacgtcgacc ttgccgtgtt cgcttaaccc gcgcctggca tagctgcgtc cccgtcgcat
     2281 ccaatcgtcc cggctatgca acctggtgat attccaatcc atcagccgct gcagccaggt
     2341 cggcaaaccc agaatcatcg gattgacacc aacctgcgca tccgggtagc tttgcttaat
     2401 cagttggcga gctgcggtat gcgcctggaa gagattacgc atctac
//
LOCUS       DASZAK010001142         3974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_348538, whole genome shotgun sequence.
ACCESSION   DASZAK010001142 DASZAK010000000
VERSION     DASZAK010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348538"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2797
                     /locus_tag="VGT44_20275"
     CDS             <1..2797
                     /locus_tag="VGT44_20275"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV2583209.1"
                     /translation="NRSAYRYPSEDVVAALESDARWGFTAGEARSRLQRYGRNELPSS
                     PPVPAWQQFLAQFWNPLTILLLVATVISFVVWWIERDSAFPYESLTIFAIVLLNGILG
                     YVQEHRAERAVAALQAMTAATARVLRDGNVQTIPTTEVAPGDILVIEEGDTIAADARV
                     LEAIALRLAEATLTGESTPVSKSSTPIDQEEGIGDQTNMVFSGTAAAAGRGRAIVTAT
                     GAGTELGRIAGSLQRTVEKETPLQRELDRVGKLLGIVVMLIAIVMSVTILLVDRVGAL
                     SELVDILLLAVSLAVAAVPEGLTAITTIVLSLGMQRMAQRNVIVRKLSAVETLGSTTV
                     ICTDKTGTLTRNEMTVRAVVTASGRVDFTGAGYDPRGVALQAGQPLNNPVLLEEVETA
                     LRAAYLVNNATLLSRDGIWTIQGDPTEGALLVAAHKVGLEEEDLEGRFPRMAEVPFSS
                     ERKLMSTSHEDTAHNERLIIFSKGAPDVLLSRCTYERFGPGGQARPLTTERRTEILAD
                     VEHLAAEALRTIGVALRTLPREKIDEAGELDERVEQEFTWLGVIGMIDPPRPEAAESV
                     RLAQLAGVRVIMITGDHPITAEAIARELGIIKANERTMTGAKLEKASEEELRGIVRDI
                     SVYARVAPEHKLRLVQALKANGEIVAMTGDGVNDAPALKMADIGVAMGMAGTDVAKDA
                     ADMILTDDNFASIVAAIEEGRAIFANIQKFLRYLLSSNIGEVLTMFFGVILGSAIGLT
                     HEVGTAIVVPLLSTQILWINLLTDSGPALGLGVDPRDPRQMGRPPRNPKNRVISNTMW
                     VGIIIVGVIMTISSLLIIDWSLPGGLIPGQGTLHYAQTMTFTTLVVAQLFNVFNARSD
                     VTSAFRSLFTNKWLWLAILISLLLQIAVVYIPLLQTAFKTVPLQITDWLFCLVVSSMV
                     LWGRELTKLAMLQPRIP"
     gene            3003..>3974
                     /locus_tag="VGT44_20280"
     CDS             3003..>3974
                     /locus_tag="VGT44_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583210.1"
                     /translation="MSSISQVSDALKRVLTRRAKELERETGFVERSTAQLDGPTFVQT
                     TVFGWMDNAEASYAQLRHVAASLGVSVSTQAVEQRFGTESAALLREVLQEAVGEVLSS
                     EASAPALLSRFNGVYVQDGTVITLPAELRDEWRGCGGSTPEAGQSGMRVQIRLDLAQG
                     GMQGPWLQEGRAAERSGEAHEAPLPQGCLYNVDAGYFTLAEMRAHGQAGCYWLTTANA
                     GTPLIDERGQCWDLVSFLRAQTGKEVDVQVLLGKYERLPVRLIARRVSPEQAERRRKS
                     ANRQQEVKAKGVQRPNVRKRRGAGDKRKRTRKRRKTGKARLQLLDWTI"
BASE COUNT          847 a         1074 c         1314 g          739 t
ORIGIN      
        1 gaatcgctcg gcctatcgtt acccctcaga ggacgtggtc gcagcgctgg aaagtgacgc
       61 caggtggggg ttcacggcgg gggaggcgcg gtcccgcctg caacgctacg gcaggaatga
      121 gttaccgagc tcgcctcccg tcccggcctg gcagcagttc ctggcccagt tctggaatcc
      181 gctcaccatc ctgctgctcg tcgccaccgt catctcgttc gttgtctggt ggatcgagcg
      241 agacagcgcc tttccctacg agtcgctgac gatcttcgcc atcgtgctgc tcaatggcat
      301 tctgggctat gtgcaggagc accgggcaga gcgggcagtc gcggcactac aagctatgac
      361 cgcggcgacc gcgagggtgc tgcgcgatgg aaacgtgcaa accatcccga caaccgaagt
      421 ggctccgggt gacattctcg tcatcgagga aggggatacc attgctgccg atgcgcgcgt
      481 gctggaggca attgcactgc gcctggcgga ggcaactctg acgggcgaga gtacacctgt
      541 ctccaagagc agcactccga ttgaccagga ggaaggcatc ggggatcaga ccaacatggt
      601 cttcagcggc acggcggcag cggcgggacg cgggcgtgcc atcgtcacgg cgacgggagc
      661 cgggaccgaa ctaggacgga tcgcaggttc gctccagcgg acagtggaga aagagacgcc
      721 gctccaacgg gaactcgacc gggttggaaa attgctgggc atcgttgtaa tgctcatcgc
      781 gatcgtaatg agcgtgacga tcctgcttgt agaccgggta ggggccctca gcgaacttgt
      841 cgatattttg ctgctcgcgg tctcgctggc ggtggcagcc gtaccggagg gattgactgc
      901 tatcacaacc attgtcctct cgctcggcat gcagcgcatg gcacagcgca acgtcattgt
      961 gcgcaagcta tcagcggtcg agacgctcgg atcaacaacg gtgatctgca ccgacaagac
     1021 gggaacactg accaggaacg aaatgaccgt gcgggcggtg gtgacggcca gtgggcgcgt
     1081 agacttcact ggtgcgggct atgatccgcg cggagttgcc ttgcaggcgg gacagccgct
     1141 caataatccc gttctcctgg aagaagtgga aacggcgctg cgcgcagcct acctggtgaa
     1201 caatgcgacg ctgctttctc gtgatggcat ctggaccatc cagggagatc cgaccgaagg
     1261 cgctttgctg gtcgcggcgc acaaggtagg cctcgaggag gaggacctgg agggacgctt
     1321 cccacgcatg gcagaggtac cgttctcctc tgagcggaag ttgatgagca cgtcacacga
     1381 ggacacagct cacaacgagc gtctgatcat cttttccaaa ggcgcccccg acgtgctgct
     1441 atcccgctgc acatatgaac ggtttggtcc cggaggccag gctcgacctc ttaccacaga
     1501 gcgccgcact gaaattctag cggatgtaga acacctcgcg gctgaggctt tgcgcacgat
     1561 tggtgtggct ttgcgtacat taccgcgcga gaagatagac gaagcggggg aactcgacga
     1621 gcgcgtggag caagagttta cctggcttgg ggtgatcggc atgatcgacc cgccgcgacc
     1681 agaggccgcg gaatcggtgc gtctggcaca gttggcgggg gtcagggtga tcatgataac
     1741 gggcgatcac ccgatcactg cagaagcgat agcgcgcgaa ctaggcatta tcaaagcaaa
     1801 cgagcgtaca atgaccgggg ccaaactgga gaaagcaagc gaagaagagc tgcgtgggat
     1861 tgtgcgcgat atctcggtgt acgcgcgggt ggcacctgaa cataaactgc gcctcgtgca
     1921 ggcgctcaaa gctaacggtg agatcgtcgc catgactggc gatggagtca acgacgcgcc
     1981 cgcgctcaag atggctgaca tcggagtagc tatgggcatg gcaggaacag atgtcgcaaa
     2041 agatgccgcc gatatgattc tgaccgatga caactttgcc tcgattgtcg cagcaataga
     2101 ggaggggcgc gcgatcttcg ccaacataca gaagttcttg cgctacctgc tctcctcgaa
     2161 tattggcgag gtgctgacga tgtttttcgg cgttatcctg ggcagcgcca ttgggctcac
     2221 ccacgaagta ggtactgcga tcgtggtgcc gctgttgtcg acgcagatcc tgtggatcaa
     2281 cctcctgacc gattcggggc cggcgcttgg cctgggggtt gatcctcgcg atcccaggca
     2341 gatgggcagg ccgccacgca atccaaagaa tcgcgtcatc tcgaacacga tgtgggtggg
     2401 catcatcatc gtcggcgtga tcatgactat tagcagtctg ctcatcatag actggagctt
     2461 gcctggtggg ctgattccag gccagggcac cctgcactat gcacagacca tgacatttac
     2521 cacgctcgtg gttgcacagt tgtttaacgt ttttaacgcg cgttccgatg tgaccagcgc
     2581 gttccgctcc ctttttacga ataaatggct ttggctggct atcctgatat cgctgctgtt
     2641 gcagattgca gtggtgtaca tcccgctgct ccagaccgcc tttaagacgg ttcctttaca
     2701 aatcaccgac tggctgttct gtctcgtggt atccagtatg gtactctggg gacgagaact
     2761 gacgaaactg gcgatgctcc agcccaggat tccgtagggg cgaccttcat ggtcgcccgg
     2821 gggtggggct gctggctgtt ccgttcagcg atgatccacc atcttcaggc ggcccacggc
     2881 gggcgaccat gaaggtcgcc caataggcat caacctaagg aaggaaaagc cggaaaagaa
     2941 gactcctgaa gtgctagaat cttagtattg aagcaagatc tagcagcaac aggaggaaaa
     3001 tcatgagcag tatatcacag gtcagcgatg cgttgaagcg cgtgttgacg aggcgcgcca
     3061 aggaactgga acgggagacc gggtttgtgg agcgcagcac ggcgcagttg gatgggccca
     3121 cgtttgtgca aacgacggtc tttggctgga tggacaatgc ggaggcgagc tacgcccagt
     3181 tacggcacgt agcagcgagt ctgggagtgt cggtgagtac gcaggccgtg gagcaacgct
     3241 ttgggaccga gtcagcagca ttgctgcgcg aggtgttgca ggaggcggtg ggggaagtgc
     3301 tcagcagtga agcgagcgcg ccagcgttgc tgagccgctt caatggagta tatgtgcagg
     3361 atggtacggt gatcacgctg ccagcggagt tgcgcgacga gtggcgcggc tgtggtggca
     3421 gcacccccga agcgggacaa agtggcatgc gggtgcaaat acgtctggac ctggcgcagg
     3481 gaggcatgca agggccatgg ctgcaagaag gacgagccgc tgaacgcagt ggagaggcac
     3541 acgaggcccc attgccccag ggctgtctct acaatgtgga tgcgggctat ttcacgctcg
     3601 ccgagatgcg agcgcatggc caggcaggct gctactggtt gacgaccgcc aacgctggga
     3661 cgccactcat cgatgagcgc gggcagtgct gggatctggt gtcctttctg agagcgcaga
     3721 cgggcaagga ggtggatgtg caggtgctgc tcgggaaata cgaacggctg ccagtgcgac
     3781 tgattgccag gcgcgtgagt cccgagcaag ccgagcgacg acgcaagagc gccaatcgcc
     3841 agcaagaggt gaaggccaaa ggggtgcagc gacccaatgt gcgcaaacgg cggggagcag
     3901 gggacaagcg caagcggact cgcaagcgca gaaagacggg caaggcgcgc ctgcagttac
     3961 tggactggac gatt
//
LOCUS       DASZAK010001143         3558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_598086, whole genome shotgun sequence.
ACCESSION   DASZAK010001143 DASZAK010000000
VERSION     DASZAK010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3558
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598086"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VGT44_20285"
     CDS             <1..298
                     /locus_tag="VGT44_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEV2583211.1"
                     /translation="EDIVDRGQTLHYLMDVLRQRKPLSLRVCALLDKGRERIKPVHLD
                     YTGFQIPDRFVVGYGLDYAQRYRNLPYIGILKPSVYQDEIVVPQKQHKSPAEDH"
     gene            342..1352
                     /locus_tag="VGT44_20290"
     CDS             342..1352
                     /locus_tag="VGT44_20290"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV2583212.1"
                     /translation="MTAVGVIGSGAWGTTLALLLAKKGFATTLWEYRPDRAAEMQHSR
                     ENQLFLPGFVFPPALSVTSDIAEATSSKDLLLLVTPSQTMRKNVRLLAPYLGQQTLCI
                     SGSKGIEVGSLKRMTEVIADELPNAAHRVAALSGPTISLEVAQGRPTAAVIAALDEQV
                     AVRVRDLLATPAFRIYTSNDVIGVELGGALKNIIAIGAGFNEGMQYGENAKAAFITRA
                     LAEIARLGIAAGAQPLTFAGLAGIGDLIATCASPLSRNQQLGRRLAAGETLEYIMAST
                     HSVAEGIYATRAALQLAARFNVELPITRLLSQVLFEGLDPHKAVPELMMRDPKSELEG
                     MG"
     gene            complement(1359..2639)
                     /gene="tyrS"
                     /locus_tag="VGT44_20295"
     CDS             complement(1359..2639)
                     /gene="tyrS"
                     /locus_tag="VGT44_20295"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV2583213.1"
                     /translation="MNAYEEFQWRGMLYDASEGLREALAKESLTGYIGFDPTAASLHV
                     GSLLPIMGLMRLQQYGHTPIALVGGGTGMIGDPSGKTVERQLLSKEQLEFNLAGIREQ
                     LSRFLDFERKTNAAMLVNNADWLGPLGLIDFLRDIGKHFTVNYMLAKESVRRRYEQGD
                     GISFTEFSYMLLQSYDFLVLHDRYGCTLQLGGSDQWGNIVAGAELIRKLRGEQGGRGF
                     GLVFPLVMTSSGIKFGKSEAGTVWLDANLTSPYRFYQYWINTDDRDVVKYLKFFTMFN
                     QDEIAALEASVEIAPEKREAQRQLAQDVTRRVHGEHELRKAMQASQVLFGGEIADLSA
                     RDVLDIFAEVPSSDLPRASFEGDGLPIIDAVLAAGYATSRGAARRLIEGGGIYVNNRR
                     VSNPQATLRLSELIDGQIMVLRKGAKDYHLIRAV"
     gene            3023..3319
                     /locus_tag="VGT44_20300"
     CDS             3023..3319
                     /locus_tag="VGT44_20300"
                     /inference="COORDINATES: protein motif:HMM:NF023159.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV2583214.1"
                     /translation="MPDIATDTTQQPLQAILKQFQTIPGIGKSIALDLWNQGYRSLDE
                     LKGQDPEEMYVRQCALQGGPVDRCLLYTFRCAVYYVSNEQHEPELLKWWNWKDR"
     gene            3322..>3558
                     /locus_tag="VGT44_20305"
     CDS             3322..>3558
                     /locus_tag="VGT44_20305"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEV2583215.1"
                     /translation="MERLNISTGTTWEPIVGYSRAVRVGNIVHVAGTTATDAGGNIVG
                     IGDPYTQAVQILKNIETALTRAGASLKDVVRTRIY"
BASE COUNT          755 a         1173 c          977 g          653 t
ORIGIN      
        1 agaagacatc gttgataggg gccagaccct gcactattta atggacgtgt tgcgacagcg
       61 caagccgctt agcctgcgcg tctgcgcact gctcgataaa gggcgagagc gcatcaagcc
      121 tgtccacctc gattacaccg gcttccagat acccgaccgt ttcgtcgtcg gctacggcct
      181 ggactacgcc cagcgctatc gcaatctgcc ctacatcggc attttgaaac cgtcggtcta
      241 ccaggacgaa atagtcgtcc cgcagaaaca gcataaaagc ccggcggaag accactaatc
      301 cactcacgat catgggcatg tatctagagg gagagccagc gatgacagca gtcggcgtaa
      361 tcggcagcgg ggcctgggga actaccctgg cgctcctgct ggcgaagaaa ggcttcgcca
      421 ccaccctgtg ggaataccgg cccgatcgcg ccgccgagat gcagcacagc cgcgaaaacc
      481 agctcttcct gcccggcttc gtctttcccc ccgcgctcag cgtcacctcc gacattgcgg
      541 aggcgacgag cagcaaggac ctgctgctgc ttgtcacgcc ctcgcagacg atgcgcaaga
      601 acgtgcgcct gctcgcgccc tacctcggcc agcaaacgct ctgcatcagt ggcagcaagg
      661 gcatcgaggt tggcagcctc aagcgcatga cggaggtcat cgccgacgag cttcccaacg
      721 cagcccatcg cgtcgcggcg ctgagcggcc ccaccatctc gctcgaagtg gcgcagggca
      781 ggcccaccgc cgccgtaatc gccgccctgg acgagcaggt cgcagtgcgc gtccgcgacc
      841 tgctggctac cccggcgttt cgcatctata cctcaaatga tgtcatcgga gtggaactgg
      901 gaggcgcgct gaagaatatc atcgccatcg gcgcgggctt caacgagggt atgcagtatg
      961 gcgagaacgc caaggctgcc ttcatcacgc gcgcgctggc cgaaatcgcg cgcctgggta
     1021 tcgcggcggg cgcgcaaccg ctcaccttcg cgggtctggc gggcatcggc gacctgatcg
     1081 ccacctgcgc cagcccgctc agccgtaacc agcagctagg ccgccgcctc gccgccgggg
     1141 aaacgctgga gtatatcatg gcctccaccc acagcgtcgc cgagggcatc tacgccacca
     1201 gggccgccct gcaacttgcc gcgcgtttca acgtcgaatt acccatcacc cgcctgctca
     1261 gccaggtcct ctttgaaggt ctcgatcccc acaaagccgt gccggaattg atgatgcgcg
     1321 acccaaagag cgaacttgaa ggcatgggat aagcgcgcct acaccgctcg aataaggtga
     1381 taatccttcg cgcccttgcg cagcaccata atctgcccat cgatcagctc tgagagcctc
     1441 agcgtggcct gcgggttgga gacgcggcga ttattgacgt agatgccgcc gccctcgatc
     1501 aagcgccgcg ccgcgccgcg cgaggtggca tagcctgccg ccagcactgc gtcgatgatg
     1561 ggaaggccat caccctcgaa gctagcgcgg ggcagatcgc tggatggcac ttcggcgaag
     1621 atgtcgagca catcgcgcgc gctcaggtcg gctatctcgc cgccgaacag cacctgcgat
     1681 gcctgcatcg ccttgcgcag ttcatgttcg ccatgcacgc ggcgcgtcac atcctgcgca
     1741 agctggcgct gcgcctcgcg cttctccggc gcgatctcga cggacgcctc cagcgctgcg
     1801 atctcatcct ggttgaacat tgtaaagaac ttcagatact tcaccacgtc gcgatcatcg
     1861 gtattgatcc aatactggta gaagcgatac ggcgaggtca ggttggcgtc cagccagacc
     1921 gttccagcct ccgacttgcc gaacttgatg cccgacgagg tcatgacgag agggaagacc
     1981 aggccaaagc cgcgcccgcc ctgctcgccg cgcagcttgc ggatcagttc agcgcccgcc
     2041 acgatattgc cccactggtc gctaccgccc agttgcagcg tgcagccata gcggtcgtgc
     2101 agcaccagga agtcgtagga ttgcagcagc atatagctga attcggtgaa cgagatgccg
     2161 tcaccctgct cataacgccg cctgaccgac tccttcgcca gcatatagtt gacggtaaaa
     2221 tgcttgccga tatcgcgcag gaaatcgatc aggccaagcg gcccaagcca gtcagcgttg
     2281 ttaaccagca tcgccgcgtt ggtctttctc tcgaaatcca gaaagcggct caactgctcg
     2341 cgaatgcccg ccaggttgaa ctccagttgc tccttagaaa gcaactgccg ctcgaccgtc
     2401 ttgccgctgg gatcgccaat catccccgta ccgccgccca ccagcgcgat aggggtatgc
     2461 ccatactgct gcaagcgcat tagccccatg atgggcagca gcgagccaac atgcaggctg
     2521 gccgccgtcg ggtcaaaacc tatatagccc gtcagcgact ccttcgccag cgcttcccgc
     2581 aagccttcgg atgcatcata gagcattcca cgccactgaa attcttcgta cgcattcata
     2641 acggtaatct acctcttcgt tcctcacaat gtggtctgat gttatcatgc catgatgtcc
     2701 cggctattgt agcagtctat catggcaacc gcaagggttg ccactacgat aacggtttgg
     2761 tttggtacat ccccattatc gtagtggcaa cccttgcggt tgccataccg cccatatcgc
     2821 tactgacata ctgttgtcac cagcatatgc tacactaagc tcagtgctaa catcctggtt
     2881 gttcgtcccg gtgcaatcgg tgtgtagggg cgcaatgaat tgcgccctcg tcccggtcgc
     2941 atcggcctgt caagcgggcg caatgaattg cgcccctaca cacttggtcc accttgacag
     3001 cattattgca aaggagccag ccatgccaga catcgcaacc gatacaaccc agcaaccgct
     3061 gcaggccatt ctcaaacaat tccagactat ccccggcata ggcaaaagca tcgcgcttga
     3121 cctttggaac cagggctatc gctcgctgga tgaactaaag ggccaggacc cggaagaaat
     3181 gtacgtgcgc cagtgcgccc tgcaaggcgg cccggttgat cgctgcctgc tgtacacctt
     3241 tcgctgcgcc gtctactacg tctccaacga gcagcacgaa ccggaactac tgaaatggtg
     3301 gaactggaaa gacaggtaag catggagcgc ttgaatatct caaccggaac aacctgggaa
     3361 cccatcgtcg gctattcgcg cgccgtgcgc gtcggcaaca tcgtacatgt agcaggcaca
     3421 accgcgaccg acgccggtgg caacatcgtc ggcatcggcg atccctacac ccaggctgtg
     3481 caaatcctca aaaacatcga aaccgccctc acacgggcag gcgcgagcct caaagatgtg
     3541 gtgcgcacgc gcatctac
//
LOCUS       DASZAK010001144         2182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_312968, whole genome shotgun sequence.
ACCESSION   DASZAK010001144 DASZAK010000000
VERSION     DASZAK010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312968"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            674..1267
                     /locus_tag="VGT44_20310"
     CDS             674..1267
                     /locus_tag="VGT44_20310"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2583216.1"
                     /translation="MTEHQLSLLPFYAGWGIYQQRLVAAIAPLTGEQLALRNTPQNWS
                     IGMYATHIVADRAWWFHARMGEGSADLTSLELWALGICEGGVDPFHPAAELVAGLEKT
                     WQMIQKTLARLTPADLEQVFPSLDEAERVRHAKLVEPALQPFAQMWLDAAALSGEVRP
                     AVSRQQIIWGVLEHDIHHGSEISTILGVHGLPVVELD"
     gene            complement(1672..1971)
                     /locus_tag="VGT44_20315"
     CDS             complement(1672..1971)
                     /locus_tag="VGT44_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583217.1"
                     /translation="MTVFPFTVAGAVGTVKVLAVVRVTAAIGALVAGVVVGPLLVVPQ
                     LLTVPTSTGARTMSQAFFGTLLIRVPQSKQGARLVSSSPQACARKRCAPATDTTM"
BASE COUNT          429 a          665 c          588 g          500 t
ORIGIN      
        1 cggagctccc aatttttttt gccatgtggc aagtattttg ctggtccgcc cgtgtcatgt
       61 gcctgtgtat ctctcctcta ccgatccaag cgtcccggat cgagttcgcc aggttcgcca
      121 acccatcctt gacaggatca tctctctccc tgtatagtag tgaagtagtc actcgttttt
      181 tgggaggttt tgacatctat gccagtgtgc cgctaactcc atcctgtttc ctgttcaaac
      241 agtgaaacag acgttcccat tcttgcttcc atctctcgcg tgtctctcat cagacatgag
      301 cgctcagccg ttctacgcct ggaatgcgag aggcgcatga ttcctgctcg acggccccac
      361 gggcctcgcc tgcgcgagac ggcggatagt gagaacgcaa gcatttgatg gtgggaagcc
      421 gatatggaga agcgtgcggc aaagaggtgt caggcatgga actccggcga acgcatcgct
      481 gatgttcccc atccaccata ccattggcag atggatgcca tcttttcccc tgtcgtggca
      541 gccgcatatt cgatcattgt gcaagcgatg acctgctctc tctcctccct ctggtctctc
      601 ccatcgtttc ctcgcgcgag ttgatacgat cgtgctgttt tttctcgaag acaaaaagca
      661 aggaatacct atcatgacag aacatcagct ttcactcctc cccttctacg cgggttgggg
      721 tatctaccag cagcgtcttg tcgcggccat cgcgcccctt acaggagagc agcttgcctt
      781 gcgcaacacg ccgcaaaact ggtcgattgg catgtacgcg acgcatattg tcgcagatcg
      841 cgcctggtgg ttccacgcac ggatgggcga gggaagcgcc gatcttactt ccctcgaatt
      901 atgggctctc ggaatctgtg aggggggcgt ggatccattc cacccggcgg cagaactggt
      961 tgccggactt gagaagacct ggcagatgat ccagaagacg cttgcccgcc tgacccctgc
     1021 tgatcttgag caggttttcc cgtctctgga tgaggcggaa cgtgtgcgtc acgcgaaact
     1081 ggtcgagcct gcgctccagc cattcgccca gatgtggttg gatgcggcgg ctctttctgg
     1141 agaagtgaga cccgcagttt cgcgccagca gatcatctgg ggtgtgctcg aacatgacat
     1201 ccatcacggc agcgaaatct ccaccattct cggcgtccac ggcctgccag tcgtcgagtt
     1261 ggattaagaa caggaacgtc ttcagttccc ctctctcatg ggtggatagg ttgcgcatga
     1321 tgcggtcgct gaagctccaa ggtcgcccct ggagcttcag cgaccttcca tgctcatttg
     1381 ttttaaccat aatacggcct attaggcccg ccaacgaatt ttacaacgtt aaggtcgttc
     1441 ccaacgagat ggcagcaagt agacagtata agcggcttcg gatgccttga ctcaagactc
     1501 cgactctctg ctccgccgtg gccttctttc cgtgagacgc tcctccccct catggcagcg
     1561 ctgtctctct tgccccctgg tgatcgttct gctcaggtcc atgccaccgg gcctttgtaa
     1621 cctcctgacg tattacctat agtagggcaa gtgtgcgccc agactcgtga gctacatcgt
     1681 tgtgtccgta gcgggtgcgc accgcttcct cgcacacgcc tggggggagg aactgaccag
     1741 gcgagcgcct tgcttactct gggggacacg tatgagaaga gttccaaaga atgcctggct
     1801 catagtccgc gctcctgtgc tggtggggac cgtaaggagt tgcggcacga ccaggagtgg
     1861 gccaaccacc accccggcca cgagcgcccc gatggcggct gtcaccctga ccacggccag
     1921 caccttcacc gtgccgaccg cgccagccac ggtgaacggg aaaaccgtca cgatcttgac
     1981 tgacggtcaa ggcaggaccc tgtactcctt cacccctgat gcagcctcaa aaacgctatg
     2041 caccggcagt tgtgcccaga cctggccccc cgctgctctt caccggaaca ggcaagccca
     2101 cggcctccac ggtactactg ggggaatgag aagtgtacaa gcatgccaat ggcgagcagg
     2161 tcatctgaaa tgaccatcct tg
//
LOCUS       DASZAK010001145         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_457181, whole genome shotgun sequence.
ACCESSION   DASZAK010001145 DASZAK010000000
VERSION     DASZAK010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457181"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="VGT44_20320"
     CDS             complement(<1..684)
                     /locus_tag="VGT44_20320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583218.1"
                     /translation="MTERLQIRVSRVIRARLCWCAIASLLVLTRLGATREKGGAADDQ
                     GREYAAEAPKTILELQQFRQSHSIQIRSKGGRQGAATLVDLNPAINVWYLLKVVWKDG
                     GPELAYHLENPKPHARKLLLDEKYSAGLVVAEGKNRYFCDLFGADALGQAKATGLIFS
                     PMCEGHVYLRNPATGHRTNLEAATELLRQHVWGAEKIIALGHILMGDVHRETGKIQTA
                     AGAKTGREAG"
     gene            complement(681..2153)
                     /locus_tag="VGT44_20325"
     CDS             complement(681..2153)
                     /locus_tag="VGT44_20325"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEV2583219.1"
                     /translation="MTGAADFRPINSAEQSIQSPPLVVDLDGTLVKTNLLLEAALALL
                     KEKPQYIFVLPVWLLKGKAYLKQQVTRRVSLDVSVLPYREEFLDYLKAQRTEGRSLVL
                     ATAGNLQIARQVAEHLKLFDLVFASDGTTNLSGESKRDRLAREFGEKGFDYAGNDRHD
                     LVVWSSARKAIVVNPTRLASSGVASVAQVDRIFIFEDPGKRRVDYLKPLRPQHWLKNL
                     LIFVPLLAAHRFEEIALSGKALLAFLAFGCVASAGYLINDLLDLQADRHHPGKRFRSF
                     ASGDLPLSYGLAMIPALVGLGYLIGMLVSPLFLYVLLIYLALSLTFSLHVKKVAVLDV
                     ILLAGLYTMRIMAGCAAIAIWPSHWLLAFSIFLFFSLALVKRYGELVIMRTIDGDGAK
                     ARGYELSDGELLAAMGTASGYLAVLVLALYISSNTAQVLYGRYELIWFLCPLLLYWIS
                     HLWLTAHRGRMPDDPVVFAISDRVSRILMLLMLATTVLAL"
     gene            2620..3153
                     /locus_tag="VGT44_20330"
     CDS             2620..3153
                     /locus_tag="VGT44_20330"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV2583220.1"
                     /translation="MLVKDLMTRNVSSCRQEDNLAELAKVMQKQRCGALPIVDGSGRV
                     TGIITDRDICIALGTRNLRASDVRVRDVSPPGCISCSPDNDVRDALRKMASQEVSRLP
                     VVDEVGQLVGIISIDDIIFRAGGGRSGLTDREIIDTMRAMREERIHQPEAAAKEGSNL
                     SPDIRMPMNYEHIRSKK"
BASE COUNT          762 a          898 c          870 g          640 t
ORIGIN      
        1 gcccgcttct cttccagttt tagcgcccgc ggcagtctga atctttccag tctcacggtg
       61 tacatctccc atgaggatgt gaccgagagc aatgatcttc tccgcgcccc aaacgtgctg
      121 gcggagaagc tctgttgcgg cctctagatt tgtgcgatgc ccggtcgcgg gattgcgaag
      181 atacacatgg ccttcgcaca tgggggagaa gatgaggccc gtggctttgg cttgacccaa
      241 agcatcggct ccaaacagat cgcagaaata tcgattcttc ccctccgcga ccaccagacc
      301 cgccgaatat ttctcatcca ggaggagttt tctggcgtgc ggcttcggat tctccagatg
      361 ataggccaac tccgggccgc cgtctttcca tacaaccttg agcaggtacc atacgttgat
      421 cgcaggattc aggtcgacca gagtagccgc gccctgtctt ccccccttgg atctgatctg
      481 gatggaatgc gattgccgaa actgctgcag ttccagaatg gtcttgggcg cctcggcggc
      541 gtactccctc ccttgatcgt ctgctgcgcc ccctttttcc cgagtggccc caagccgtgt
      601 gaggacaagc aggctcgcaa tagcgcacca gcaaaggcgt gctcgtatga ctcgactcac
      661 gcggatctgc aagcgttccg tcatagcgcc aggacagtcg tggccaacat gagcagcatc
      721 aggatgcggc tgacccggtc gctgatggca aaaacgaccg gatcatcagg catccgccca
      781 cgatgagccg tcaaccagag gtggctgatc caatagagca acagtgggca gagaaaccat
      841 atcagttcgt agcgcccgta caagacctgc gctgtattgc tgctgatata gagggcgagc
      901 accagcaccg ccaaataccc actggcggtt cccatagccg ccagcagttc tccatcgctg
      961 agttcataac ctcgtgcctt ggcgccatcg ccgtcgatcg tccgcatgat caccagttcc
     1021 ccatagcgct tcaccagtgc caggctaaag aacaggaaaa tcgaaaacgc caataaccag
     1081 tgcgacggcc agatagcaat ggccgcgcaa cctgccatga tccgcatagt gtaaagaccg
     1141 gccagaagga ttacgtccag aacagcaacc ttcttcacat gcagagaaaa ggtcagactc
     1201 aaggcgagat agatcaacaa tacgtacagg aagaggggcg agaccaacat gccgatcagg
     1261 tacccaaggc caaccagagc tggaatcatc gcaaggccgt aggagagcgg gaggtcgccg
     1321 gacgcgaaag accggaatcg cttccctgga tgatgccgat ctgcctgcag gtcgagcaag
     1381 tcattgatca ggtacccagc cgacgcgaca cacccgaacg ccagaaacgc cagtagcgcc
     1441 tttcccgaca aggcaatctc ttcgaaacga tgggccgcca ggagaggaac aaaaatcagg
     1501 aggttcttca gccagtgctg gggacggagg ggcttcagat agtcaactcg acgttttccc
     1561 ggatcctcga agatgaagat gcggtcgact tgcgctacac tcgccacacc tgaacttgcc
     1621 agccgggtgg gattcaccac aatggctttc cgcgctgaag accaaaccac tagatcgtga
     1681 cggtcattgc cggcataatc gaagcccttc tccccgaact cacgcgcgag acgatcccgt
     1741 ttggactcac cggaaagatt ggtcgtccca tcgctggcaa agaccaaatc gaagagcttc
     1801 aaatgttccg ccacctggcg ggcgatctgc aggtttccag cagttgccag aaccagggag
     1861 cgtccttcag tgcgctgcgc tttgagatag tccaggaatt cctctcgata cgggagaacg
     1921 ctgacatcca aggagacccg gcgggtcact tgctgcttaa gataggcttt ccccttcagc
     1981 aaccagacgg gcaagacaaa gatgtattgc ggtttctcct ttaacagcgc cagcgcagcc
     2041 tccagcagga gattcgtctt caccaaggtg ccatccaggt ccaccactag gggcggagat
     2101 tggatggatt gctctgccga gttgatcggc ctgaagtctg cggcgcctgt cattaggaat
     2161 actgctacac gatgactgag cagttttcgc gccaatgccc acgctgccct tgatgaacgg
     2221 ctgccatatc tttcgcgatc gagctgagac tgagcttaat cattgcgcta ctacacccat
     2281 gcggggcaag aatagcgccg ccacacacga ccaatggcag gagaatctta ggtcagatct
     2341 cttcctagtc agattggaac ttcgcatgct gcatgccgga tgtgcctgtg gctgtcttcc
     2401 cagccttcca aacgaaacaa gatattcccc tgatcacact atttaccgaa gctatcgcgg
     2461 gtactcgatg tccctcacgg gtgctgctca tggaccaatc ggaagattgg ctgtctgaca
     2521 cgcgggggca ttggcaaacc gtcttttcag taacttaaat tgctccagca tttctaaggt
     2581 ggaagcagag ttgcacgcag ttgtaagagg gaggttgcca tgctagtgaa ggacttgatg
     2641 accagaaacg taagctcctg ccgtcaggaa gacaatctgg cagagttagc gaaagtcatg
     2701 cagaagcagc gatgcggcgc cctgccaatt gtggatggct ccggtagggt gacgggcatc
     2761 attacggatc gagatatctg tattgccctg gggactagga acctcagggc gtcggacgtg
     2821 cgtgtacgag atgtttcacc tccggggtgc atcagttgca gtcccgacaa tgatgtgcgg
     2881 gatgctctgc gaaaaatggc ctcacaggaa gtcagccggt tgcctgttgt cgatgaagtc
     2941 ggtcaactag ttggcattat ttcgattgac gacatcatct ttcgcgcagg cggcggacgt
     3001 tcaggtctca ctgaccgcga aatcatcgac accatgagag cgatgcggga ggaacgcatt
     3061 caccaaccgg aggcggccgc gaaagaggga agcaatttga gtccggatat cagaatgccg
     3121 atgaactacg agcacattag aagcaaaaaa tgatcgtcca gcaacaggct
//
LOCUS       DASZAK010001146         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_313003, whole genome shotgun sequence.
ACCESSION   DASZAK010001146 DASZAK010000000
VERSION     DASZAK010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313003"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1462
                     /locus_tag="VGT44_20335"
     CDS             89..1462
                     /locus_tag="VGT44_20335"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEV2583221.1"
                     /translation="MTQVEQLAAFAVQTRYEDLSEQARHQLKIRILDALGCAFGALEG
                     PPIKMLRSQLEDFGGRPLVSLIGGNKTAPDRAALYNSALVRYLDYNDSYIAKKETCHP
                     SDNLGAVLAASEYAHRNGKEFLTALALAYQVQCRLSDVAPVRAKGFDHTTQGAYSVAA
                     GVARALGLDQRGIANAVAMSGTAYNALRVTRTGSLSHWKGLAYPNTAFGATHAAFLAM
                     RGVTGPLEVFEGNKGLMEAITGPFELDWSKETLEAVRRTSVKKYNAEFHSQSALEGIL
                     ELRAAHHILPEEIEEIKIDIFDVAFHIIGGGEEGDKQRVRTKEEADHSLPYMVAVALL
                     DGDVSPGQYSPERIVREDVQSLLRRVTILPDEALSKRFPDQMPCRIAITLKDGRTLRI
                     EKQDYEGFYARPMSWEKAAAKFELLAAPFTEAIQRAAIVETVANLETKGVAQLTGLLR
                     AENAPIR"
     gene            1485..2306
                     /locus_tag="VGT44_20340"
     CDS             1485..2306
                     /locus_tag="VGT44_20340"
                     /inference="COORDINATES: protein motif:HMM:NF014709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphosulfolactate synthase"
                     /protein_id="HEV2583222.1"
                     /translation="MSDRAFPFIRLNEREGKPRARGITEIRGPYYTPMGKRYLEDVLE
                     TMGEYADSLKFAGGSFTLMPEKELKAIIDLAHRYNVLVSTGGFIEHVLTQGPEVVNRY
                     IEECRNVGFDILEISSGFITIPVDDWLRLVEKVQHAGLKAKPEVGIQFGAGGASAAEE
                     LESEGTRDVDWAIGQARRFLDAGAYMIMIESEGITENVKTWRTDAAARIIGALGLEKV
                     MFEAADPEVFAWYIKNYGPEVNLFVDHSQIVQLECLRAGIWGTKSLWGRVMTYNG"
     gene            complement(2338..3312)
                     /locus_tag="VGT44_20345"
     CDS             complement(2338..3312)
                     /locus_tag="VGT44_20345"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2583223.1"
                     /translation="MQAMVFIQFGGPEELVLTEIPTPVPENGEVLIKVKALGINRAEV
                     YMRMGLFGEVTPVSGIECVGQVEYDPSGTLYSGQTVATITGGMGRTRNGSYAEYTCVP
                     IQNVYPLETDLDWATLAAIPESYATAWSCLFANLRISAGQVLFVRGGTSALGQAAINI
                     AKQEGVTVLTSTRSAIKVALLTELGAERVLIENGNLSEEVRAFYPDGIDGVLDIIGNS
                     TLLDSLRMARKGGRVCVAGFLGSSEPVAFSWLANMPFGVDLNAFASLFFGTKDFPHSD
                     VPMQKIVDRVANGIYKSKPVKVFPFEHIPEAHRLMESNSANGKIVVVR"
BASE COUNT          815 a          934 c          940 g          748 t
ORIGIN      
        1 aatattatat caaacaggtg ttagacgtat caactattct ggcttatact ttctctatag
       61 tcattttcgg agtagaaaag agaaagatat gacacaggtt gaacaactgg cagcttttgc
      121 cgtgcaaacg aggtatgaag acctttcaga gcaagcgcgt catcagctca agattcgcat
      181 actcgatgcc ctcggctgcg cctttggggc gctcgagggt cctcctatta agatgctgcg
      241 ctcccagctc gaagacttcg gcggcagacc cctggtctcg ctgatcggcg gcaacaaaac
      301 agctcccgac cgcgccgctc tttacaactc ggcgctggtt cgctacctgg actacaacga
      361 tagctacatc gccaaaaagg agacctgcca ccccagcgac aacctcggcg cggtcctcgc
      421 agcaagcgaa tacgcgcaca gaaatggtaa ggaattcctg accgctctgg ccctcgccta
      481 ccaggtccag tgccgtctct cagacgtagc cccggtgcga gcgaagggct tcgatcacac
      541 gacgcagggt gcctactctg tagcggctgg cgtagccagg gcgcttggtc tcgaccagag
      601 agggatcgcg aacgccgttg cgatgagcgg aacagcctac aatgccctgc gcgtcacccg
      661 cacgggaagc ctctctcact ggaaagggct ggcttatccc aataccgctt ttggcgctac
      721 ccacgccgcc ttcctcgcca tgcgcggtgt aaccggtcct ctggaagttt tcgaggggaa
      781 caaaggcctg atggaagcca ttaccggacc tttcgagtta gactggagca aggagacgct
      841 tgaggccgtg cggcgcacca gcgtcaagaa gtacaacgcg gagtttcatt cgcagtccgc
      901 gcttgagggt atcctggagc tgcgtgcggc ccaccatata cttcccgagg aaattgagga
      961 gatcaagatc gacatttttg atgttgcgtt tcacatcatc ggcgggggcg aagagggaga
     1021 caagcagcgg gtgcgcacca aggaagaggc cgatcacagc ctgccctaca tggtagcagt
     1081 cgcactgctg gatggtgatg tttcgcctgg tcagtactcg cctgagcgca tcgtgcgcga
     1141 ggatgtgcag agcctgctgc gcagagtaac gatcctgccc gatgaggccc tgagcaagcg
     1201 cttcccagac cagatgccct gccgcatcgc gatcacactc aaagatggtc ggacactgcg
     1261 tattgagaag caggactacg agggatttta tgcgcgaccg atgtcttggg agaaggctgc
     1321 ggccaagttt gagctcctgg cagccccctt taccgaagca atccagcgtg ccgccatcgt
     1381 agagacggta gccaacctgg aaacgaaagg tgtagcgcag ttgaccgggt tattacgcgc
     1441 agaaaatgcg ccaatccgtt aatcggagaa agagagacaa tacaatgagc gatcgagcat
     1501 ttccctttat tcgcctgaat gagcgcgagg gcaagccacg cgcgcgcggt atcaccgaga
     1561 ttcgcggccc ctactacact ccaatgggca agcgctacct ggaggatgtc ctggagacga
     1621 tgggcgaata tgccgattcg ttgaaattcg cgggtggctc ctttaccctc atgcctgaga
     1681 aagagctgaa agcgattatt gatcttgccc accgctacaa cgtgctggtc tccaccggcg
     1741 gctttatcga gcacgtgctg acgcaggggc cagaagtcgt gaaccgctat atcgaggagt
     1801 gtcgcaacgt gggtttcgac attcttgaaa tctcgagcgg ctttatcacc atcccggtcg
     1861 acgattggct gcgcctggtt gagaaggtgc agcacgctgg tctgaaggcg aagccggagg
     1921 ttggcatcca gttcggcgcg ggtggagcca gcgcagccga agaacttgaa tcagaaggga
     1981 caagagatgt ggactgggcc attgggcagg ccaggaggtt tctcgatgcc ggggcctata
     2041 tgatcatgat cgagtccgaa ggcatcaccg aaaacgtaaa gacgtggcgc accgatgccg
     2101 cagccaggat catcggtgct ttaggattgg aaaaggtgat gttcgaggct gccgatccgg
     2161 aggtctttgc ctggtacatc aagaattatg gccccgaggt caatcttttt gtggatcaca
     2221 gccagattgt ccagctcgaa tgcctccgcg cgggcatctg gggaaccaag agcctctggg
     2281 ggcgcgtcat gacctacaac gggtagaatc gaaagggtac tgatgattta cattcatcta
     2341 gcgtacgaca acgatcttcc cattagcgct atttgattcc atcagacgat gggcttcagg
     2401 aatgtgttca aatgggaaga ccttcacagg cttcgactta tagatcccat tagccactcg
     2461 atcaacgatc ttttgcatag gaacatcaga atgtggaaag tccttcgttc caaaaaagag
     2521 gctcgcgaaa gcattcaaat cgacgccaaa gggcatattc gccaaccaac tgaatgcaac
     2581 aggctcgctg ctcccgagaa atccggcaac acaaacacgg ccacctttcc ttgccatacg
     2641 caatgaatca agcagtgtgc tattgccgat gatatccaga acgccgtcga tgccatcggg
     2701 atagaaagct cggacctcct cgctgagatt cccgttctca atcaggactc gttcagcgcc
     2761 gagctcggtg agcaacgcca ccttgattgc gcttctcgtt gaggtgagga ccgtcactcc
     2821 ctcctgtttg gcaatgttga tggcagcctg tccaagggcc gatgttccgc cacgcacgaa
     2881 cagcacctgg cctgccgaaa tgcgtaaatt tgcaaagaga caactccagg ctgtcgcgta
     2941 agattctgga atggcagcca gggttgccca gtccaggtca gtttcgagag gatagacgtt
     3001 ttgtataggc acacaggtat attcggcata gctcccgttg cgcgtgcggc ccattcctcc
     3061 cgttattgtc gccactgtct ggccggaata cagagtcccc gatggatcat attccacctg
     3121 tcccacacat tcgatcccac tcaccggtgt gacctcccca aagaggccca ttcgcatata
     3181 cacttccgct cgattgatgc ccagggcttt gactttgatc aagacttcgc cgttctctgg
     3241 aactggcgtt ggaatctccg taagcactaa ctcttctgga ccaccgaact ggatgaatac
     3301 cattgcttgc atttgatagc tccttctatc gtttgtatta actttatatg ttagtattta
     3361 ctaataacgt taatattata gaaggcaata agcgttttgt caaggacagg tgcggaaaac
     3421 attgattgaa atcattg
//
LOCUS       DASZAK010001147         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_101997, whole genome shotgun sequence.
ACCESSION   DASZAK010001147 DASZAK010000000
VERSION     DASZAK010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101997"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VGT44_20350"
     CDS             <1..192
                     /locus_tag="VGT44_20350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583224.1"
                     /translation="RVAIVPTGLSYSRTRGRRWQVAVRYGPARFRSEFDNAEQLLHAV
                     EEDVHALSNPTPPALPPAQ"
     gene            complement(332..1582)
                     /locus_tag="VGT44_20355"
     CDS             complement(332..1582)
                     /locus_tag="VGT44_20355"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2583225.1"
                     /translation="MSHRFKSLLLTVGFCALLAPLLASCDIQTAPSHPQQPRPVSVAA
                     CHGTLRPASATGTPSRSVPRSIYFGTSQGELYAVNAQTGSPRWCITLISVNHTSASVG
                     TPAIVDGVVFVCASIDGQTNGSLYALNASDGALRWQTRTYCSIGGLGDAGLPLVNSGV
                     VYSGYYAVREQDGQPLWEVPSALVQESIFLAVSNGVLYGCTYGNVFALNTANGSMLWR
                     YPPYKFNIQGAKVVSPQLIVVGTQEPVNVQTSALYVLNTDTGTLRWYFLMGSYVGATY
                     LNNVVYVGAGDGYVYAFDAKSGNVLWHDHFATGAFTYGAFAPALSNGVLYINGDGAYA
                     LRSDTGSVLWHNTLGTKNSEITPPASYDYVSFQPSTVSNGIDYVAITGRGAATTLYAL
                     NASTGAEEWHSQLFPQLYSSLAVA"
     gene            complement(1630..>2465)
                     /locus_tag="VGT44_20360"
     CDS             complement(1630..>2465)
                     /locus_tag="VGT44_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583226.1"
                     /translation="AALAECLFSTDSRLCTLIDTVGVGKPVMLSSQAGGLKITMWITP
                     GPYFLGEMLVADLSLTNYAETTYLLQGWPSVPGFETQAACHPPFAVHVTGGSPTDPAI
                     GNAIAYLANCWDGPGSNGTVQVQPGQTINFHQVIALTSSGNVTLSVQGVFQQAVLETN
                     GVNIVNGTGPLDGHWPALHITVASQSPPARVISLQRDGLQVRVVAPPALNNQMLYWPT
                     TSCGHAGGGSSWAAWIALPSETITPDCGGPVTVTPELVNSWVFIVGAPGYAFALEKYP
                     S"
BASE COUNT          524 a          732 c          721 g          488 t
ORIGIN      
        1 cgtgtagcga tcgtcccgac cgggcttagc tattcacgca cgcgcggcag gcgctggcaa
       61 gtcgccgttc gctacggtcc cgcgcggttt cgcagcgaat tcgataacgc cgaacaactg
      121 ctgcatgccg tcgaggaaga tgtgcatgcc ctctcgaacc ctacgccgcc tgccctacct
      181 cccgcgcaat gaaccttcac cctcacccca tcagggtgcc tctgccccac ctgcgtggtc
      241 tgatttgtgc gttaccagct tcgccctcct caagggaaga cacgctcacc atctgctcgc
      301 tctttgcgct ttttaccacc tatctcgtct atcaagccac cgccagcgat gagtagagct
      361 gggggaacaa ctggctgtgc cattcctccg ccccggtgct ggcattcagc gcgtagagcg
      421 tggtagccgc acctctaccc gtgatggcta catagtcgat gccattcgag acagtagatg
      481 gttgaaaaga gacgtagtca taactggccg gaggagtaat ctcgctgttc ttcgtaccga
      541 gagtattgtg ccagagcaca cttcccgtat cgctccgcag ggcgtatgcc ccatcgccat
      601 tgatgtagag tactccgttc gagagcgctg gggcgaatgc gccataggtg aaagctccag
      661 tggcgaagtg gtcgtgccag agcacatttc cgctcttggc atcgaatgca tacacatatc
      721 catccccagc gcccacataa actacattgt tgaggtacgt cgctcccaca tacgacccca
      781 tcaggaaata ccagcgcagc gtgccggtgt ccgtgttgag gacatagagg gcactggttt
      841 gcacattaac tggttcttgt gttccgacca cgatcagttg aggggagacc acctttgcac
      901 cctggatgtt gaacttgtag ggagggtagc gccagagcat cgagccattc gccgtattga
      961 gcgcaaaaac attgccgtag gtacagccgt agagaacccc attggacact gccaggaaga
     1021 tgctctcctg gacaagcgcg gaagggacct cccacagcgg ctggccgtcc tgctctcgga
     1081 cggcgtagta acctgagtag acaaccccac tgttcaccag tggaagtcct gcatctccaa
     1141 ggccgccgat cgaacaatac gtcctggttt gccagcgcag cgctccatcg ctggcgttga
     1201 gcgcgtacag ggacccatta gtctgcccgt caatggacgc gcagacgaag actacgccat
     1261 cgacaatagc gggcgtcccg acggacgctg atgtgtggtt caccgagata agcgtgatac
     1321 accagcgcgg acttcccgtc tgggcgttga ctgcgtaaag ctcaccctgg ctggtgccaa
     1381 aatagatcga gcgtggtact gaccgggagg gagtgccggt ggccgaggcc gggcgcagcg
     1441 tcccatggca ggcagcgacc gacacagggc gcggttgttg ggggtgggat ggtgccgtct
     1501 gaatatcgca gctcgcgagc aacggagcaa gcagggcgca gaaacccact gtaagcagta
     1561 gggatttgaa ccgatgtgac atcaccaaat tccttccatg tgcacctttt cagagcttaa
     1621 tcagcccggt caggatgggt acttttccag ggcgaaggca tagccgggcg caccgacgat
     1681 gaaaacccag ctattgacca attcaggagt gaccgtgaca ggaccgccgc aatcaggcgt
     1741 gatggtctcc gacggcaggg caatccaggc agcccaggaa gagcctcctc cggcatggcc
     1801 acaactggtg gtcggccagt agagcatctg gttgttcagt gcgggcggcg cgacgacacg
     1861 tacttgcaac ccatctcgtt gaagcgagat gacacgcgcc ggtggggact gactcgcgac
     1921 cgtgatgtgc agggcgggcc agtgcccgtc gagcggtccg gtgccattga cgatattgac
     1981 gccatttgtc tccagcacgg cttgttgaaa gacgccctga acgctgaggg tcacgttgcc
     2041 gctgctcgtc agcgcaatga cctggtgaaa gttgatggtc tggccaggtt gtacctgcac
     2101 cgtcccattg gaacccggcc catcccaaca gttggcgaga taggctatcg catttccaat
     2161 cgccgggtca gtcgggctac cacctgtcac atggacagca aagggcggat gacaggcggc
     2221 ttgggtctcg aatcctggta ctgacggcca tccttgtagc aagtaggtag tctcggcata
     2281 attggtcagc gagaggtccg ccaccagcat ttcgccgaga aagtaagggc cgggcgtgat
     2341 ccacatcgtt atcttcaatc caccggcctg tgaactgagc atcaccggct ttcccacccc
     2401 tacagtatcg atcaacgtgc aaaggcgact gtcagtggag aacaggcatt cggccagggc
     2461 cgccg
//
LOCUS       DASZAK010001148         1766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_561680, whole genome shotgun sequence.
ACCESSION   DASZAK010001148 DASZAK010000000
VERSION     DASZAK010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561680"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VGT44_20365"
     CDS             <1..888
                     /locus_tag="VGT44_20365"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV2583227.1"
                     /translation="YARTTPANKLRIVQVLQQQGERVAVTGDGINDAPALAAADIGIA
                     MGETGTDVAREAADIVLEDDNFTTIVNAIREGRVLFGNLKKGVRYYLACKAALVSVVL
                     LPVLLGVPVPFAPIQIILMELFMDLAASATFANEPAEADVMHEPPRNPKARFMDKPMV
                     ASIFGAGAGLFAAVSVAYLVTWYTSGGDLVRAQTVAFVAWLLGHVFLAINLRSEREPL
                     SRLGFFSNRLMILWAVAAIAFVLFATLVPGVQNLLKTTTLSGSDWLLVLIATVIGTFW
                     IEIPKVILWRGHSQREGTA"
     gene            1161..>1766
                     /locus_tag="VGT44_20370"
     CDS             1161..>1766
                     /locus_tag="VGT44_20370"
                     /inference="COORDINATES: protein motif:HMM:NF019634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583228.1"
                     /translation="MSTISRRETILLGWYVAVAAVGTAADVYRELFLHFFSRRWQYDY
                     TVKAIHRLDPRALEFILAPTARPLQFVAGQFVFVHFGGSAEWERHPFTVSSAPQERDL
                     RLAQALGDYTQHLSDTLQPGAPAIVSIAFGLFDYRQGTREQVWIAGGIGITPFRSWIR
                     SFPPEQQIEFDIDLYYTVRTEDEALFLDEIRTAATQHPSFRP"
BASE COUNT          329 a          524 c          538 g          375 t
ORIGIN      
        1 tacgcgcgca cgactccagc aaacaaactg cgcattgtgc aagtcttgca acaacagggg
       61 gaacgggtgg ctgtcaccgg tgatggcatc aatgacgctc ctgccctggc cgctgctgac
      121 attgggatag cgatgggcga gacaggcacc gacgtggccc gcgaagcggc cgacatcgtg
      181 ctggaggatg acaacttcac caccatcgtc aacgcgatca gagaaggacg ggtgctgttt
      241 ggcaacctca agaaaggggt acgctactac ctggcctgca aagccgctct ggtgtcggtg
      301 gtcttgctgc cggtgctctt gggcgtgccg gtcccctttg cccccatcca gatcatcttg
      361 atggaactct tcatggacct ggccgcctcg gcaaccttcg cgaacgaacc ggctgaggcc
      421 gatgtgatgc acgaaccgcc gcgcaaccca aaggcccggt tcatggataa gcccatggtg
      481 gcaagcatct tcggtgccgg ggccggattg tttgccgctg tctctgtggc ctatctcgtt
      541 acctggtaca cgagtggtgg cgacctcgtg cgggcgcaaa cggtcgcgtt tgtggcgtgg
      601 ttgctggggc acgtctttct ggcgatcaat ctgcgttcgg aacgggaacc gctctcccgc
      661 ctgggcttct tctccaaccg gctgatgatt ctctgggcag tggcggccat cgccttcgtc
      721 ctctttgcga ccctggtccc tggggtgcag aacctcctca agacgaccac gctcagcggt
      781 tcggactggc tcctggtgct gatcgcgacg gtgattggaa cgttctggat cgaaattcct
      841 aaagtgatcc tttggcgagg ccattcccaa cgggaaggaa ctgcctgacg gttcgactgc
      901 gctactgatt ggagatgaga gaatgttaac aataaccaat tatgatgcca tcataacttc
      961 ctgaacgtgg gagcgcgacc ggctgtgccg tccttgagcg gccttgaaca ggtcaacgcg
     1021 ttgaattcga cgacgatcat gttatgctat gagcgtactc gagcaccgca tagcagagtg
     1081 gactccgttc catgtgtgat cacatggtca ggcgttctcc agtacacggg gcaagttggt
     1141 gcagtgaaat gtcctggccc atgagcacga tttccagaag agagacgatc ctgctggggt
     1201 ggtacgtggc ggtggcggcg gtcgggaccg ccgccgacgt gtaccgggag ctgtttctcc
     1261 acttcttctc gcggcggtgg cagtacgact atacggtgaa ggcgatccat cggctggacc
     1321 cgagagcgct cgagttcatc cttgctccca cggcccgacc cctgcagttt gtcgcggggc
     1381 agttcgtttt cgtgcacttc ggtgggagcg ccgagtggga acgccaccct ttcaccgtca
     1441 gcagtgcccc gcaggaacgc gatcttcgcc tcgctcaggc gctgggcgat tacacccagc
     1501 acctctcgga caccctccaa cctggggcac cggcaatcgt gagtattgcg tttggtctgt
     1561 tcgattaccg ccaaggtacc cgtgagcagg tgtggatcgc tggcggcatc gggatcactc
     1621 cgtttcggag ctggatacgc tcgttccccc cagagcagca gatagagttc gacatcgacc
     1681 tgtactacac cgtgcgtacc gaagacgagg cgctgttcct cgacgagatc aggacggcgg
     1741 ccacacagca cccatccttc cggcct
//
LOCUS       DASZAK010001149        11175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_279149, whole genome shotgun sequence.
ACCESSION   DASZAK010001149 DASZAK010000000
VERSION     DASZAK010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 11175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11175
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279149"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1008
                     /locus_tag="VGT44_20375"
     CDS             187..1008
                     /locus_tag="VGT44_20375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2583229.1"
                     /translation="MNQRISFTVEKNRVLVPVGEPCPFGCRYCYTRGGEVGLSRVSAE
                     DVLSRFRQFALEASFDTIQFGYDGDPFARPERGITMLKQLATMHKHVNFSTKAFLEGA
                     TIDALHEIRRYMETAGTTLSALVSLSCWDSASAVEPHTPTPRERIMTIENLTRIGIPA
                     FIAVRPVLPHILDSEYERIAVEGIRAGCEGFILGPLYADARGRFVRFIPPATLAKVPG
                     RTGSVSWSAHAPMWTRYEDPARLQRLLSMIERKGGRTFLSSADAVALVSQMERIA"
     gene            1005..1634
                     /locus_tag="VGT44_20380"
     CDS             1005..1634
                     /locus_tag="VGT44_20380"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV2583230.1"
                     /translation="MIEVELKFEVPTASRALFQAELENLPKVRWFGQIYNSDSYYDTA
                     NFDCLQQAVFIRVRNHEQLEMKFHDRADPGHTHSTERSFLLESPRMKEINLLCSRFIS
                     RWQAANSIEEAFRINGLMEYVHIANKRTQYEHGNLMLSVDDVEGLGNFFEIETACEEG
                     TEIEQALTRLRGFVSDFAFPALRPVKTGYVEQWLRLHNPQVYQLRKVPA"
     gene            1631..1933
                     /locus_tag="VGT44_20385"
     CDS             1631..1933
                     /locus_tag="VGT44_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583231.1"
                     /translation="MSEILSDVAYFRKQQALSEEAALLGLTGFAITATHESITARMER
                     CAARILCLIDEGRHEEAQAILNTDSWYADDQACTGEQTGIPTGKKARAEAQEHEPA"
     gene            2422..4872
                     /locus_tag="VGT44_20390"
     CDS             2422..4872
                     /locus_tag="VGT44_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV2583232.1"
                     /translation="MTSHTTAGLERRREDYTLITGQGRYVDDLRPPPGRPATLHMAVV
                     RSPYAHAAIQEIRLDAARALPGVVAAFGADELVASMPNMDGLPIPPGLKQTPRKPLAV
                     QKVRYVGDPLAVVLAENLYAAWDARDLVEVDYQPLPVVADPEAALAPGAPILYEEFGT
                     NLGFNAQSSGGDIAATFAQAEHTVRLRVVNQRVAASSLEPRACLFDFDPASGQFTAWV
                     STQAIYSTRDMLAQFLGLDRNKIRVYNADVGGGFGTKTSFVGEEIIAAALAVRYGQPV
                     KWIETRSENLQSQTHGRGQINYIDAAFQSDGRLLGLRVRTIADLGGFLARSTTLVPNG
                     TSSMLNGPYRIQAIQSQVLGAFTNKVPTGAYRGAGRPEAAYILERTMDRIAHELGLDP
                     VEVRRRNLIAPDAFPYQTLTGMAYDSGNYQATLDAAIKLADYQDWRAKQQQQRAQGNA
                     KMLGIGLATFVEVSGGPMGGPRAPKEAATVRIRSDGSILVQSGVATNGQGHFTAFAQI
                     AATIFNLPGTQIEVQMNDSALPAFGFGTFGSRTLQTAGAAVHQAAEAARAKALSVAAR
                     HLEAALADLELANGRVSVRGVPSRAVTLGELARMVEERPDLIEHERPNPANGTPIEGL
                     AAWRDFSPPNAGFSSGTHLAVIEVDSETGDINILNYVAIDDCGTVLNHYLADAQAHGA
                     LAQGIGQALYEETLYNDDGQLLSSTLMDYTMPNAQQVPSFQTTFVETPSPNNPLGAKG
                     IGEGGTIGAPPTIVNAVLDALAPLGIKTLDMPLRPEKVWEKIQAAKEGTLNEGEPTVP
                     EVFDKVQGALEGGSSVFV"
     gene            5013..5615
                     /locus_tag="VGT44_20395"
     CDS             5013..5615
                     /locus_tag="VGT44_20395"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEV2583233.1"
                     /translation="MTNILTMFSASHVVQGIIVGGVLAFLTAQIMMNAVVKAMTTTVN
                     GWSTTFKCGQPGNGILLRAACARNLPAVNVVQEAAYWTTTVDSAGRRLSGQRAYVLHF
                     PAGQLPPNDAFWSLTMTDVVGFMVSNPIDRSSVGSRSGLVPNADGSIDIAIQRVAPIG
                     HESNWLPAPAGYFKLMLRAYLPGRAILDGEYHVPPVMKVP"
     gene            5617..6273
                     /locus_tag="VGT44_20400"
     CDS             5617..6273
                     /locus_tag="VGT44_20400"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HEV2583234.1"
                     /translation="MNRLILKYKYPITFAILAVLAWVVYRYFLMGGSQLITFVVVAVL
                     VWGLGTCVFIYFWPPITYSAYKRAVVQHGLGGGPIPVNTLYAVPTLSSPSASNSSLLA
                     TGTNDVLYVGGLLDLSKGAQVLHVPDMAGRYYSVQFTDPSDGTNFAYVGKRTTGTEAG
                     DYLMSGPGWKGAVSQGMTQIASPNNSVLVIGRVFVESDSDLSTAYGLAKQIQLTPLSH
                     "
     gene            6412..9537
                     /gene="fxsT"
                     /locus_tag="VGT44_20405"
     CDS             6412..9537
                     /gene="fxsT"
                     /locus_tag="VGT44_20405"
                     /inference="COORDINATES: protein motif:HMM:NF040586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxSxx-COOH system tetratricopeptide repeat
                     protein"
                     /protein_id="HEV2583235.1"
                     /translation="MKNRRLKQQFGHYRLLSLLGEGGFSEVYLGEHIYLKTQAAIKIL
                     TIPLEQDERSRFLSEARIIAHLDHPHIVRVLDFGLQDRVPFLVLSYAPHGSMRQLYPK
                     GAALPLSTIVDVVRQLASALQYAHSQQLIHGDVKPENILVGRGQEVLLSDFGVAVVAS
                     HSLHRSDISGTVAYMSPEQLRGEPCFASDQYALGVVVYEWLCGSRPFIGSFTEIAMQH
                     LHTPPPSLLDRQPELSPAIEQVVLRALAKDPQQRFSNILDFAKTFVAACQPLLDTNGD
                     NIDAGKTVAMQEGAFIAAPSHEQPQQEAVFRFLPFFLPVHRNPYFTGRETLLKRLYGL
                     LREPTSRMNAHLAHAPAVAALCGMGGIGKSQIAAEYAYSYQHEYTAILWARAESHEVL
                     FADFVALAQYMKLADAVAFQPPSQNAASQQAVQAVTDWLAAHAGWLLILDNIEEMSLA
                     YRFVPPVSQGHILLTTRAQSTSTLAPRLDVEKMTIDEGALFLLYRSKMYLPGTQREQI
                     SGPDWLEAQDIYHLMDGLPLALDQAGAYIEESGCSLYRYIERFRQQRAMLLELRGEAS
                     LLHPEAVSATVLLAINKVEQSNPAAVDLLRLCAFLDSGQVNEELLEAYDLDTLVAALR
                     RYSLIVRSPDESALSLHRLVQAIIYDHMEPDMQCAWAERAVETVSQAFPPGEKVAAWP
                     RCERCMPHVYTCFQHIERWQINSPPAASLLDRAGLYLLTQGLYTQAVRLLQKALRIRE
                     TLLPQEQPEQQAMSETINSLACCYLYLGEYAQAGSMFTKALAICETLNGPSHPDVAVI
                     LNNLALTHHKCGKYAQAEQCLHQALAIWEQLPQTAHPDFARTLNDMALLHHQQGRWRE
                     AEACYRRGLVIWEQALGPSHPDLAVHLNNLAMLYAQQRDYAQADPLFRRALEIRERTL
                     GPDHPITAHSLVYLARSFEYQWRYNEAELCYTQALAIRKRALGPDHPEIAYSLCNLAK
                     LYVSQGKHAQAEPLYRQALAIREQALGSDHPEVASLLQSYAILLVSVKRNDEAIQLAR
                     RAKTIHATQHD"
     gene            complement(9681..9878)
                     /locus_tag="VGT44_20410"
     CDS             complement(9681..9878)
                     /locus_tag="VGT44_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583236.1"
                     /translation="MVPTLCKSLRKLRIVLIALPLLVLMLSLSPAPPLAHSSIPSQHP
                     RMAMFCLIGGICVDVIINVNV"
     gene            complement(10039..>11175)
                     /locus_tag="VGT44_20415"
     CDS             complement(10039..>11175)
                     /locus_tag="VGT44_20415"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV2583237.1"
                     /translation="QRGFTHMTTVVRSEPLFVSTLPGRYYYDPAIYELELEKIFSHMW
                     VCIGRADALPNPGAYRVVTLGRESVIVARNREGMLNAFLNVCRHRGARLCSQETGQLK
                     GSIQCRYHAWTYGLDGRLIGAPNVMNDERFERVTFGLFPVALEVWHGFIYLSLDEHPA
                     PVAEQLNPVILERFGSLQPFERYGVGDLKVGKSITYDVQANWKLLLENFMECYHCSPM
                     HPELCDLLPGFRTGKSYIDGDAATLAEGIEAFNMTGRASRPLLKGLRPEDGRKYYGEV
                     LLPNVLINLLGDHVVAHTVWPLAPDRSLVVCDWLFDADVAAAPGFDPMDAVELFDIVN
                     KQDWEVCELTQQSMSSRAFKNGGVYAPNERHIRAFADFVLVKLD"
BASE COUNT         2429 a         3514 c         2996 g         2236 t
ORIGIN      
        1 tattgtaaca tttcttccaa cttcatcaag ttgcccatcg tttcggaaat attcgacata
       61 ttttaaaaaa tcgcgatttt cttaacaatt gctatatttt tacgaaatat tgctaattgt
      121 cttgattatg acatttgtta tgttatactt ttcttaaagc ttgcttgcac tagtcaggaa
      181 acttgaatga accaacgcat ctcgtttacc gtagaaaaga atcgcgtact tgttcccgtt
      241 ggtgagcctt gccccttcgg atgccgctat tgctatactc gaggaggaga ggtcggtctc
      301 tcgcgtgtca gcgcggagga cgttctttct cgcttccggc agttcgccct ggaagcctct
      361 ttcgatacga ttcagtttgg ctacgacggt gatccgtttg ctcgccctga acgcggcata
      421 accatgctca agcagctggc aaccatgcac aaacacgtga actttagcac aaaagcgttt
      481 cttgaaggcg caaccatcga cgcgctgcac gagattcgcc gctacatgga aacagcagga
      541 actactttaa gtgcgctggt ttctctgagc tgctgggatt ctgcttccgc cgttgaacct
      601 cacacgccta ctccgcgcga gcggattatg accattgaga acctcacacg catcggcatt
      661 cccgcgttca tagccgtgcg gccagtgctg ccacatattc tcgatagcga gtacgaacgt
      721 atcgccgttg aaggcattcg cgcgggatgc gaaggattta tcctgggacc gctctacgcc
      781 gacgcgcgtg ggcgctttgt gcggttcatc ccacctgcga cgcttgcgaa ggtgccaggc
      841 cgcacaggaa gtgtctcctg gtctgcccac gcgcccatgt ggacacgtta cgaagacccg
      901 gctcgtctcc agcgactgct ctcgatgatc gagcggaagg gcggacgtac cttcctctct
      961 tcagcagacg ctgtagcgct tgtttctcaa atggagcgta tagcatgatc gaagtcgagt
     1021 taaaattcga ggttcccacg gcgtcacgag cgctgttcca ggcagaacta gagaacctgc
     1081 caaaagttcg ctggtttggc caaatttaca attcagacag ctattacgac acggcgaatt
     1141 tcgactgcct gcagcaggcg gtatttatcc gcgttagaaa ccacgagcag ctggaaatga
     1201 aattccatga tcgagctgat ccagggcata cacacagcac agaacggtca tttttattgg
     1261 agtcgcctcg aatgaaagag atcaatttgc tttgttctcg cttcatctca aggtggcaag
     1321 cagccaattc gatcgaagag gcatttcgaa tcaacggatt gatggaatac gtacacattg
     1381 cgaataagag aactcaatat gagcacggaa acctgatgct cagcgttgac gatgttgaag
     1441 gacttgggaa ctttttcgag attgaaactg cctgtgaaga ggggacagag atcgaacagg
     1501 cgctgacaag actgcgaggc tttgtctctg actttgcttt tcccgccctg cggcctgtga
     1561 agacaggata tgtcgagcaa tggctccgtc tacacaatcc acaggtctat caactgcgaa
     1621 aggtgccagc atgagcgaga ttctgagcga tgtagcatat ttcagaaaac aacaggcact
     1681 tagcgaagag gcggctctgc tcggcctgac cggctttgcc attaccgcaa cccatgagtc
     1741 gattacggcc cgcatggaac ggtgcgcagc acgtatctta tgcctcatcg atgaaggaag
     1801 acatgaagag gcccaggcga ttctgaatac cgattcatgg tacgcggatg accaggcatg
     1861 taccggggaa caaacaggca tcccaaccgg caagaaagcg cgagcagaag cacaagagca
     1921 tgagccagcg tgaggtgcat acaatgcctg tactttctgt caatttccat gacctggcag
     1981 cgctcaggag cgtcattctc ggctacctgt cctacgcacg ccggacgatt ccaccctcac
     2041 ggcaacggga gattcaaatt cgcttgctcg aaggattata ccagcgactg tcaggcattc
     2101 catccgactc gacggaagtc cgtattttta cttcagaata cagaaataca ggcgctcaat
     2161 aacgccatac aggttttcgc tatctttgtc cggcagaagg tttcgccatc actagaacgg
     2221 aacgagacac tggacagcct ggaacactta cgccaggcgc tggtgcaact gctttctatc
     2281 gtctaataac acctatgcat ccttgaaaca gccagggcga tggccagcat cgccctggct
     2341 gttctccttg cgctctcaca atcacttttg atatagtgaa agaacagcac attgcgaaca
     2401 ttcatcacag gagcaacaaa catgacatcc cacactactg ccggtttgga gcgcaggcgg
     2461 gaagactata cactgattac cggccagggc cgctacgttg acgacctgcg accacctccg
     2521 ggacgaccgg ccacattgca catggccgtc gtgcgcagtc cctatgccca tgccgccatc
     2581 caggagatcc ggctggacgc ggcccgcgcc ctgccgggcg tcgtcgccgc gttcggggct
     2641 gatgagctgg tcgcgtccat gcccaatatg gacggtctgc ccatacctcc tggcctcaaa
     2701 caaacgcccc gcaagccgct cgcggtacag aaagtgcgct acgtcggcga cccgctggcc
     2761 gtcgtgctgg ccgagaacct ctacgctgcc tgggatgcgc gcgacctcgt ggaagtggac
     2821 taccagcccc tccctgttgt ggccgacccg gaagcagcac tcgcgcccgg cgcgcctatc
     2881 ctctacgaag aatttggcac aaacctgggc ttcaacgcgc agagcagtgg gggcgacatc
     2941 gcggcaacat ttgcgcaggc cgagcatact gtgcggctgc gcgtcgtcaa ccagcgcgtc
     3001 gcggcaagct cattggagcc gcgcgcctgt cttttcgatt tcgacccggc cagcggccag
     3061 ttcaccgcct gggtctcgac gcaggccatc tattccacca gggacatgct ggcgcaattt
     3121 ctgggtctcg accgcaacaa gatacgtgtc tataacgctg atgtcggcgg cggcttcggc
     3181 accaagacat cctttgtcgg tgaagagatc atcgcggctg ctctggccgt gcgctacggc
     3241 cagcccgtca agtggatcga gacgcgcagc gagaacctgc aaagccagac gcatgggcgc
     3301 ggtcagatca actatatcga tgcggccttt caatccgatg ggcgactgct cggcctgcgt
     3361 gtgcgcacca tcgccgacct gggcggcttc ctggctcgct caaccacgct cgttcccaac
     3421 ggcacctcct cgatgctcaa cggtccctac cgcatccagg ccatccagag ccaggtgctg
     3481 ggcgcgttta ccaacaaagt cccaacaggg gcttaccggg gggcgggtcg tcccgaagca
     3541 gcctatatcc tggaacgcac gatggaccgc atcgcccacg aactgggtct cgatcccgtc
     3601 gaagtgcgcc gccgcaacct gattgcgcca gatgccttcc cctaccagac cctcaccggc
     3661 atggcgtatg atagcggcaa ctaccaggcc accctcgacg cggccatcaa gctggcggac
     3721 taccaggatt ggcgagcaaa acaacaacag cagcgtgccc agggcaacgc gaagatgctg
     3781 ggcattggcc tggcgacctt cgtagaggta tccggcggcc ccatgggcgg cccgcgcgcg
     3841 ccaaaagagg ccgcgacggt gcgcattcgc agcgacggca gcatcctggt acagagcggc
     3901 gtcgccacca acggccaggg ccatttcacc gccttcgcgc agatcgccgc cactatcttt
     3961 aatctccccg gcacgcagat cgaggtgcag atgaacgact ctgccttgcc cgctttcggc
     4021 tttggcacgt tcggcagccg cactctccag accgccggcg cagccgtcca ccaggcagcg
     4081 gaggcagcgc gcgcaaaagc cctctcggtc gcggcgcgcc acctggaagc agctctcgcc
     4141 gacctggaac tggcgaacgg tcgcgtcagt gtgcgcggcg tcccctcgcg agccgtgacg
     4201 ctgggcgaac tggcgcgcat ggttgaagag cgtcccgacc tgatcgaaca cgagcgaccc
     4261 aatcccgcca acggtacgcc catcgaggga ttggccgcct ggcgcgattt cagcccgccc
     4321 aacgccggct tctcctccgg cacgcacctc gccgtcatcg aggttgacag cgaaactggc
     4381 gacatcaata tcctgaacta cgtggcgatc gatgactgtg gcactgtcct caatcattat
     4441 ctggccgacg cgcaggcaca cggcgcgctg gctcagggca tcggccaggc gctctacgaa
     4501 gagacgctct acaacgacga cggccagctg ctctccagca cgctcatgga ctacaccatg
     4561 cccaacgcgc agcaagtgcc gtccttccag accaccttcg tcgaaacccc ctcgcccaac
     4621 aaccccctcg gcgcaaaagg catcggcgaa ggcggcacta tcggcgcgcc acccaccatc
     4681 gtcaacgccg ttctggacgc gctcgcaccc ctcggcatca aaaccctcga catgcccctc
     4741 cgcccagaga aagtctggga gaaaatccag gccgccaaag aaggtacact gaacgaggga
     4801 gaaccgacag tgccagaggt gttcgataaa gtgcagggcg cgctggaggg cggttcgtcc
     4861 gtgtttgtgt aggcagaatc cgaaccttcg tctgaacctt cgtagaagcc gattatgaat
     4921 attaacaacc aaagtcggta atgcctgatt gtcatcctga gcgcagcgga ctcttccttg
     4981 acgttcacag ctcgtagtag tatactcgag gtatgacaaa tatcctcacc atgttctcag
     5041 cctctcacgt cgtgcaaggc attattgtcg gcggcgtgct ggcgtttctc acggcacaga
     5101 tcatgatgaa cgcggtggtg aaagccatga ccacgaccgt caacgggtgg agcaccacct
     5161 tcaaatgcgg ccagcccggc aatggcatcc tcctgcgggc cgcctgcgcc agaaacctgc
     5221 ccgccgtcaa tgtggtgcaa gaggctgcct actggacgac gaccgtcgac agcgcggggc
     5281 ggcggctctc cggccagcgc gcttacgtcc tgcattttcc tgcaggacaa cttccgccca
     5341 acgatgcctt ctggtcgctg accatgaccg atgtggtcgg gttcatggtg agcaacccca
     5401 tcgatcgctc cagcgtcggc tctcgctccg gcctcgtgcc gaacgccgat ggctccatcg
     5461 acatcgctat tcagcgcgtg gctcccatcg ggcacgagtc caactggctg cctgcaccgg
     5521 caggctactt caagctgatg ctccgcgcgt atctgcccgg ccgtgctatc ctcgacggcg
     5581 agtaccacgt gccgccagtc atgaaagtgc catgacatga acagattgat tctgaaatac
     5641 aaatatccca ttacgttcgc catcctggca gtcctcgcat gggtcgtcta caggtatttc
     5701 ttgatggggg ggagccagct cattacattt gtcgtcgtgg ccgtcctcgt ctggggactc
     5761 ggcacgtgcg ttttcattta tttttggccg cctatcacgt acagtgccta caaacgagcg
     5821 gtagttcagc atggactcgg cggcggcccc atccccgtca acacgctgta cgcggtgccc
     5881 acgctctctt cgccctcggc gtcaaactcc agcctactcg caacaggaac caacgacgtg
     5941 ctctacgtcg gcggcttgct cgacctctct aaaggggcgc aggttttgca cgtgccggac
     6001 atggcgggcc gctactacag cgtgcagttc actgatccct cggacggcac taattttgcc
     6061 tacgtcggca aacgcaccac gggaaccgag gctggcgatt acctcatgag cgggcctggc
     6121 tggaaaggag ccgtgtcgca gggaatgaca cagattgctt cgccgaacaa ttcggtgctc
     6181 gttatcggtc gtgtctttgt cgagagcgac agtgatctct cgaccgccta cgggctggcg
     6241 aaacagatac agctcacacc actcagtcac tgataccccg atgtttttca ggattgacac
     6301 tccgtattcc tctttttcga ttttcttgca ttctgacaaa aaatacatta tgctcatcag
     6361 aggattagct cggcagttgg tgaacgtgcg gcatgaaagg agcttcccct gatgaaaaac
     6421 cgtcgcttga aacagcaatt cggacactat cgcctgctct cgctgctggg cgagggcgga
     6481 ttttcggaag tgtacctggg tgagcatatc tacctcaaga cgcaggcagc catcaagatt
     6541 ctcacgattc cactggaaca ggatgaacgg tcgcgcttcc tctcggaggc gcgcattatc
     6601 gcgcacctgg accacccaca tatcgtgcgc gttctcgatt tcgggctgca agatcgtgtc
     6661 ccgttcctcg tcctttccta cgcgccgcac ggctccatgc gccagctcta tcctaaaggc
     6721 gctgcgctgc ccctctcgac catcgtcgat gtcgtcaggc aactcgccag cgctctacaa
     6781 tatgcccact cgcagcagct aattcacggt gatgtcaagc cggagaatat cctggtggga
     6841 cgcggccagg aagtcctgct cagcgatttt ggcgtcgcgg tcgtcgcctc ccactcgctg
     6901 caccgcagcg acatcagcgg taccgtcgcc tacatgtccc ccgaacagct gcgcggcgaa
     6961 ccctgttttg ccagcgacca gtatgcgctg ggcgtcgtcg tgtatgaatg gctctgcggc
     7021 agccgccctt tcatcggttc attcacagaa atcgccatgc agcacctgca cactccacct
     7081 ccgtcgctgc tcgataggca gccagagctg tccccagcta tcgagcaggt cgtcttgcga
     7141 gcgctggcca aagacccaca acaacggttc tccaacattc tcgatttcgc gaagacattt
     7201 gtcgcggcct gccagccatt gcttgacacg aatggagaca acatcgacgc gggaaagacg
     7261 gttgcgatgc aagagggcgc gttcattgct gcaccgtcgc atgaacaacc tcagcaggaa
     7321 gcggtctttc gcttcctgcc gttcttcctc cctgttcatc gcaatcccta ttttaccggg
     7381 cgcgaaacat tgctaaaacg tctctacggc ttgttgcgcg agcctacctc cagaatgaat
     7441 gcccacctcg cacacgctcc agcagtcgcg gcgctctgcg gcatgggcgg catcggcaaa
     7501 tcgcagatcg ccgcggaata tgcctacagc tatcagcacg aatacacggc catcctctgg
     7561 gccagagccg agtcgcacga ggtacttttt gccgattttg tcgccctcgc gcagtacatg
     7621 aaactggcgg atgcagtggc attccaaccg ccttcgcaaa atgccgcgtc gcagcaggcg
     7681 gtccaggccg tcaccgactg gctggctgca catgccggct ggctgttgat tctggacaac
     7741 atcgaagaaa tgtcgctggc ctatcgcttc gttccgcccg tcagccaggg ccacatcctg
     7801 ctcacgacac gcgcgcaatc caccagcaca ctggctccac gcctggatgt cgagaagatg
     7861 acgatcgacg agggcgcgct cttcctgctc tatcgcagca aaatgtacct gcccggcaca
     7921 cagcgagagc agatttctgg ccccgactgg ctcgaagcgc aggatattta ccacttgatg
     7981 gatggcttgc cgctcgcact cgaccaggcg ggggcttaca ttgaagagag cggctgctcg
     8041 ctttatcgat acatcgaacg cttccggcag cagcgcgcca tgttgctcga actgcgcggt
     8101 gaagccagcc tgctgcatcc cgaagcagtc agcgctaccg tgctgcttgc catcaacaaa
     8161 gtcgagcaga gtaaccccgc cgccgttgac ctgctgcgcc tctgcgcctt cctggattcc
     8221 gggcaggtga acgaggaact gctggaagcc tatgacctcg atacgctggt tgccgcgctg
     8281 cgccgctact ccctcattgt acgcagtcca gacgaaagcg ccctctcgct gcatcgcctc
     8341 gtgcaggcca tcatctacga ccatatggag ccggatatgc agtgcgcctg ggctgaacgc
     8401 gccgtcgaaa ccgtcagcca ggcatttccg cccggcgaaa aggtggcggc ctggccgcgc
     8461 tgcgaacgct gtatgccgca cgtctacacc tgtttccagc atatcgagcg ctggcagatc
     8521 aactcaccgc cagcagccag cctgctcgac agggccggtc tctacctgct gacccaggga
     8581 ctctataccc aggccgtgcg cctgctacaa aaagcgctgc gcatacgcga aacgctcctg
     8641 ccgcaggaac agccagagca acaggccatg agcgaaacca tcaatagcct ggcctgctgc
     8701 tacctctacc tgggcgaata cgcgcaggcc gggtcgatgt ttacaaaagc tctggccatc
     8761 tgcgagacac tcaacggccc ttctcaccca gacgtggcgg tcatactcaa caacctggcc
     8821 ttgactcacc ataaatgcgg caagtatgcc caggctgaac aatgcctgca ccaggcgctc
     8881 gccatctggg aacaactacc ccaaacagcg catcctgact ttgcccgcac gcttaacgac
     8941 atggcgctgc tgcatcacca gcagggacga tggcgcgaag cagaagcctg ctaccggcgc
     9001 ggactggtga tctgggagca ggcgcttggt ccatcccacc ccgacctggc agtgcatctc
     9061 aataacctgg ccatgctcta cgcgcagcag cgcgactacg cgcaggccga cccactcttc
     9121 cggcgcgccc tggaaatccg cgagcgcaca cttggccccg accatcccat caccgcgcac
     9181 agcctggtct acctggctcg ttcattcgag taccagtggc gatacaacga agccgagcta
     9241 tgctacaccc aggcgctcgc cattcgcaag cgcgccctgg ggccagacca tccagaaatc
     9301 gcgtacagcc tctgtaacct ggccaaactc tacgtttccc agggcaaaca cgcccaggcc
     9361 gaaccactct accgccaggc gctcgccatc cgcgagcaag cgctcggctc cgaccatcca
     9421 gaagtagcat ccctgctcca gagctacgcc atcctattgg tcagcgtcaa acgcaacgac
     9481 gaagccatcc agctcgcccg ccgcgctaaa accatccacg ccactcaaca tgattgataa
     9541 tgaagtttta caagccagat cgggaatgtc atgcttcgct gcgctcagca tctcagtgga
     9601 gaggcgcaca gatgcttcgc tgcgctcagc atgacaactt cttcccgaac ctggttcttt
     9661 aaattcatta tcaccacata ttacacattc acatttataa taacatccac acagatgccc
     9721 ccaatcaagc agaacatagc catgcgcggg tgttgagacg ggatgctcga atgcgccaac
     9781 ggtggcgcgg gagacaggga gagcatcaga accagcaggg gcagcgcgat gaggacgatg
     9841 cgcagctttc tcaggctttt gcatagtgtt ggtaccataa gtatagacct cctgatagca
     9901 ggttgaacac gaggagattg agtcgcggtt ggcgggggga attgacgctc aaccggctag
     9961 ccgcgcacac gatttcctca tgttgtagac tttcccacta tctaccatcg acacatgcga
    10021 acatagatga aggtctattt aatccaactt caccagcaca aaatcggcga aggcgcgaat
    10081 gtggcgctcg tttggcgcgt agacgccgcc gttcttgaaa gcgcgggacg acatgctctg
    10141 ctgcgttaac tcgcagactt cccagtcctg cttgttgacg atatcgaaga gttcgactgc
    10201 atccatcggg tcgaaacccg gcgcggcggc cacgtcggcg tcgaagagcc agtcgcagac
    10261 gaccagcgag cgatccggcg cgagcggcca gacggtatgt gccaccacat gatcgccgag
    10321 caggttgatg agtacattgg gcagcagcac ctcgccgtaa tatttacgcc catcttccgg
    10381 gcgcaatccc ttgagcagcg ggcggctggc tctgcccgtc atgttgaatg cctcgatgcc
    10441 ctcggccagc gtggccgcgt cgccatcgat gtagctcttg ccggtgcgga aaccgggcag
    10501 cagatcgcat aactccgggt gcatcgggct gcaatggtag cactccatga agttttccag
    10561 cagcagcttc cagttggcct gcacatcata ggtgatgctc ttgcccacct tcaaatcgcc
    10621 gacgccgtaa cgctcgaacg gctggagcga accaaaacgc tccaggatca ccggattcag
    10681 ctgttcggcc accggggccg gatgttcatc gaggctcaag taaataaagc cgtgccagac
    10741 ctccaatgcc accgggaaca ggccaaaggt gacgcgctcg aaacgctcat cgttcatcac
    10801 attcggcgcg ccgattaagc gcccatccag gccatacgtc caggcatgat aacggcactg
    10861 gatcgagcct ttgagctgtc ccgtctcctg cgaacataat cgcgcgccgc gatgcctgca
    10921 cacgttcagg aaggcattca acatcccctc gcggttgcgc gccacgatca cgctctctct
    10981 gccaagcgtg acaacgcgat acgctccagg atttggtaaa gcatcggcgc gtccaataca
    11041 cacccacata tgcgagaata tcttctccaa ctccaactca taaatagcgg gatcataata
    11101 gtagcggcct ggcagcgtgg agacaaagag gggttcggag cgtaccactg tcgtcatgtg
    11161 cgtaaaacct cgttg
//
LOCUS       DASZAK010001150         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_134426, whole genome shotgun sequence.
ACCESSION   DASZAK010001150 DASZAK010000000
VERSION     DASZAK010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134426"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="VGT44_20420"
     CDS             <1..686
                     /locus_tag="VGT44_20420"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEV2583238.1"
                     /translation="GGRLELRPEDLRQKVRERKTRALLIFVVDGSDSVMARQLMPQMK
                     RALLAFTQHAYEKRDRVAMLVFRFAQGRVVLPPTRNFTLARRSIDALYVGGCTPLATG
                     LLEGLRLAETERMRDPNLAPVLVILTDGLANIDLSGNMRSITPRQDALSVAREIAARR
                     IPAIMIDTTVKRPASPFTSDESWQESAATNEEISPCRELARALDADYYQLCPPGNGVH
                     TNDVLLKRG"
     gene            883..1989
                     /locus_tag="VGT44_20425"
     CDS             883..1989
                     /locus_tag="VGT44_20425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEV2583239.1"
                     /translation="MKTRTAVLYAPGEPIRVEEIELDPPKEHEVQVRMVAAGICHSDY
                     HIISGELPSYLPFALGHEGAGIVEEVGSLVTSVKPGDHVVLTFIPSCGTCRYCTSGHA
                     NLCNLGASILAGPQLDGTFRMHAADGTGVGQMCVISTFSERSVVPEASVVKIEDYYPL
                     NRAVLVGCGVATGVGAVIHRAKIEPGSTVMVIGCGGIGMNAVQGAVIAGARMIIAVDK
                     VDFKLEKAKEFGATHTINSTREDPVQISKDLTWGEGVDYAFEAIATPATIGQAYACLG
                     KNSTLVVIGLTPYTAESIPIPPLDLVLWQKSIMGTIYGDSQPRNDIPNLLKMFHAGKL
                     KLNELVTRTYTLDQVNEAYADMLAGKNIRGVIEF"
BASE COUNT          413 a          643 c          592 g          386 t
ORIGIN      
        1 agggcggcag gctggaactg cgtcccgaag acctgcgcca gaaggtgcgc gagcgcaaga
       61 cacgcgccct gctgatcttc gtcgtggacg gcagcgactc ggtgatggcg cgccagttga
      121 tgccgcagat gaaacgcgcc ctgctggcct tcacgcagca tgcttacgag aagcgcgacc
      181 gcgtggcgat gctggttttt cgcttcgccc agggccgcgt cgtcctgcct cccacccgca
      241 acttcacgct cgcacgtcgt tcaatcgacg cgctctacgt cggcggctgc acacccctgg
      301 cgaccggcct gctcgaaggc ttgcgcctcg cggaaaccga gcggatgcgc gatccgaacc
      361 tcgcgccggt gctggtcatc ctgaccgatg ggctggccaa catcgacctg tccggcaaca
      421 tgcgcagcat caccccgcgc caggacgcgc tttcggttgc ccgtgaaata gccgcgcgcc
      481 gcatcccggc catcatgatc gacacgaccg tcaagcgacc tgctagccca tttacatccg
      541 acgaatcctg gcaagagtca gccgccacga acgaagaaat cagcccctgc cgggaacttg
      601 cccgggccct ggacgccgac tattaccagc tctgcccacc gggcaacggt gtgcatacca
      661 acgatgtttt gctgaagagg ggatgaagac acacgcatac tcgtaggagg aagagggcga
      721 tcgcaagggt ctcctacggt tggttgctag cacacccctg gacgtaaacc ctgttaatgt
      781 gcatatactg attggtgtac gttaccttac ctgtaggttg ttgtccaacg acgcccgcaa
      841 gcattccaaa gcgggaattc acttgctcag gaggtacatt ccatgaaaac acgaaccgcc
      901 gttttgtacg cgcccggtga gccgatccgc gttgaagaga tcgagcttga cccgcctaag
      961 gaacatgaag tgcaggtacg tatggtcgcc gccggtatct gccactccga ctaccacatc
     1021 atttcgggcg agctgcccag ctatctcccc tttgcgcttg gtcacgaagg tgcgggcatc
     1081 gtggaagaag tcgggtcgct tgttaccagt gttaagcctg gcgatcacgt ggttctgacc
     1141 ttcattccgt cctgcggcac ctgccgctat tgcacgtcgg gtcacgccaa cctatgcaac
     1201 ctgggcgctt ctattttggc agggccacag ctggatggta ccttccgaat gcacgctgca
     1261 gacggtactg gtgtcggcca gatgtgcgtc atttctacct ttagcgaacg ctccgtcgta
     1321 cccgaagcat ccgtcgtcaa gattgaggat tactacccgc tcaaccgcgc cgtgctggtg
     1381 ggctgcggcg tcgcgaccgg cgtcggcgcg gtcatccatc gcgcaaagat cgagccgggt
     1441 agcaccgtca tggtgatcgg ctgcggcggc atcggcatga acgctgtgca gggcgctgtc
     1501 atcgccggtg cgcgtatgat tatcgccgtt gataaggtgg acttcaaact ggaaaaagct
     1561 aaagagttcg gcgcgacgca caccatcaac tccacgcgcg aagacccggt acagatttcc
     1621 aaagacctga catggggcga gggcgtggac tacgcctttg aagccatcgc cacacccgcc
     1681 acgatcggcc aggcgtatgc ctgcctcggt aaaaacagca cgctggtggt gatcggcctg
     1741 acaccctaca cggcggagtc gatcccgata cccccgctcg atctcgtgct gtggcagaag
     1801 tccatcatgg gaacgattta cggcgactcg cagccgcgca acgacatccc caacctgctg
     1861 aagatgttcc acgcgggcaa gttgaagctg aatgagctgg tgacgcgcac ctacacactc
     1921 gaccaggtca acgaagccta cgccgacatg ctggccggta aaaacattcg cggcgtcatc
     1981 gaattttaac aaccagcgcc ggatgcccgc gcgcgggcat ccggcgctgg ttgt
//
LOCUS       DASZAK010001151         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_64226, whole genome shotgun sequence.
ACCESSION   DASZAK010001151 DASZAK010000000
VERSION     DASZAK010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64226"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            276..620
                     /locus_tag="VGT44_20430"
     CDS             276..620
                     /locus_tag="VGT44_20430"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2583240.1"
                     /translation="MDEWRELKTTLKALGDMARLTIVYHLAHAQEVTVTDLADLLGIS
                     QPLVSWHLRKLRRAGIISTRRVGRQSYCSLDTLAIRHGMQLLERLIDPAFAPETYPIG
                     TELLEAEVGLEE"
     gene            792..1595
                     /locus_tag="VGT44_20435"
     CDS             792..1595
                     /locus_tag="VGT44_20435"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HEV2583241.1"
                     /translation="MPQRQTSEMEYSNQLNSGTAMLKKRRRMTLRQAYTLGKLGRADL
                     HMHSTYSDGIGSIEQILRHVQQCTNLAVMALTDHDVIEGALRARDLWAMGNYHFDFIV
                     GEEISTLEGHLLALFIEKRIRPGLSMERSIDLVHEQGGLAVVAHPLNRIFRHSCHREV
                     LDRIARSRNTWLDGIETWNASFCGIYANRVAMSTNRRVYGWPELGNSDAHTLSSIGSG
                     YTWFEGQTAQELRECVEAGLTAPGGRLWSVNDYVHLAGHHLNKRRLRVA"
     gene            1663..>2462
                     /locus_tag="VGT44_20440"
     CDS             1663..>2462
                     /locus_tag="VGT44_20440"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV2583242.1"
                     /translation="MFSRRIQQRVRQFVTSIMQPLARLGITPNTLTVLGLLLSCITAA
                     VIAQGSLFIGGLLVLFAGIFDMFDGALARAQNTASTFGAFFDSTLDRYSESIILFGLL
                     WYALHTQALQNSFLIRWPPGNEQAWMISLIYAAVVGSLMVSYAKARAEGLGLECKTGL
                     LARPERVVILAVGLITGTGIWAVLLLAVFSNVTAVERIAYIWRVTRRTTRQRAHSQAT
                     AAGQVGAFSADGHAEVIEPWEDKALNTNRQGSVGASEGVEPSIQPVRT"
BASE COUNT          564 a          640 c          705 g          553 t
ORIGIN      
        1 atgggcgcag ttcaccgagc tcgacggcaa cgaagacggc gtctgggcgc ctgcctggta
       61 aattcccctt cccattggat agagggccga tgcaaagttg tgcgaggcat ggcgatccct
      121 tgcgggcgcc atgcctcgca caatcgtttc tcgccttttg ccgcccttct tcacgaacct
      181 tgataacatc accttgattt ttttgccata agcgggtaat atataaatat ctctttatat
      241 aaatgttcat ttatgtgaac atgataggca ggcatatgga cgaatggcgc gaactgaaaa
      301 ccaccttgaa ggcgctaggg gatatggctc gcttgacgat tgtctaccac ctggcgcacg
      361 ctcaggaagt gaccgtcacc gatctggcag acttactggg catcagccag ccgctggtat
      421 cgtggcattt gcgtaaattg cggcgagcgg gcatcatctc gacgcgccgc gtggggcgac
      481 aatcatattg ctccctggac acccttgcta ttcgtcatgg catgcaactg cttgagcgac
      541 tgatcgaccc cgccttcgcg ccagagacct atccaattgg aacagaattg ctggaagccg
      601 aagtcggatt agaggaatga gcattacagc gagtccgtca tgctgctctc gcggtgaaga
      661 gagacgatat tcactttatg aattgttaag cttaaaactt aacatttctg tgctagtgtt
      721 gtagtgaggc gcgatggaga ggcgcattta agggttgata ggtaactaga aagcttttac
      781 aaggattaga gatgccccaa agacaaacga gtgagatgga gtactcaaat caattgaatt
      841 cgggaactgc catgttgaag aagcgacgta gaatgacgct acgccaggca tacaccctgg
      901 gaaagctggg gagggccgac ctgcacatgc acagcaccta tagcgatggc atcggcagta
      961 tcgagcagat cctgcggcac gtccagcagt gtaccaacct ggctgtcatg gcgcttaccg
     1021 atcacgatgt gatcgaggga gcattacgcg cgcgcgatct ctgggccatg ggcaactacc
     1081 actttgattt tatcgttggc gaagagatca gcaccctgga aggccatttg ctggcgctgt
     1141 tcatcgagaa gcgcattcgg cctggcttga gcatggagcg cagcatcgat ctggtacacg
     1201 aacaaggtgg gctggcggtc gtagcgcacc ccctcaaccg tatctttcgt catagctgcc
     1261 atcgcgaggt attggatcgc atcgcccgct cgcgaaatac ctggttggat ggcatagaga
     1321 catggaatgc cagcttctgc ggcatttatg ccaatcgcgt cgccatgtct accaatcgcc
     1381 gcgtgtacgg ctggccagaa ctgggcaaca gcgacgccca tacgttgagt tccatcggca
     1441 gcggctatac ctggttcgag gggcaaacgg cgcaagaact gcgcgaatgt gtcgaggccg
     1501 ggctgaccgc accgggcggc agactctgga gcgtcaatga ctacgttcac ctggccggtc
     1561 atcacctgaa taaacgccgg ctgagagtgg catgatttgt gaggacacga tggaatcgcg
     1621 tccctactag cccaatttta ggaagtatgg tacaataagg tcatgtttag cagacgcata
     1681 cagcaacgag tgcggcagtt tgttacatca atcatgcaac cgctcgctcg tctgggtatc
     1741 acgccaaata ccttgaccgt gctgggactg ctgctcagtt gtataaccgc agcggtcatt
     1801 gcgcaagggt cactttttat tggcggcctg ctggtgttgt ttgctggaat ttttgacatg
     1861 ttcgatggag cgttagcgcg cgcccagaat accgccagca cttttggcgc attctttgac
     1921 tcaacgctag accgctactc agagagcatc atcctgttcg ggttgctctg gtatgccttg
     1981 cacacgcagg cgctgcaaaa cagctttctc attcgctggc cgcctgggaa cgagcaagcc
     2041 tggatgatta gcctgattta tgctgcggtc gtcggttcgt tgatggtgag ctacgcaaaa
     2101 gcgcgcgccg aagggctggg gttagagtgc aaaaccgggc tgctggcccg tccagaacgt
     2161 gtcgtcatac tggctgttgg actcattacg gggactggca tctgggcagt attgctgctg
     2221 gccgtttttt caaacgtgac cgccgttgag agaatcgcct acatctggcg agtgacgcgg
     2281 cggaccacac gacaacgcgc acatagtcag gcaacagcag cgggccaggt cggcgctttc
     2341 tcagccgatg gacacgcaga agtgatagaa ccctgggagg acaaagccct caataccaac
     2401 cggcaggggt cggttggcgc cagtgaaggt gtagaacctt caatacagcc tgtacgcacc
     2461 ag
//
LOCUS       DASZAK010001152         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_134512, whole genome shotgun sequence.
ACCESSION   DASZAK010001152 DASZAK010000000
VERSION     DASZAK010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134512"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VGT44_20445"
     CDS             complement(<1..579)
                     /locus_tag="VGT44_20445"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV2583243.1"
                     /translation="MALLRQGYSFVAGLDEAGRGCLAGPVVAAAVILPLDDETCAERF
                     AGVCDSKQLSAQARENLYDVITRSAVAVAVGIGSVELIDERNILQATKHAMRSAFHQL
                     TPAPHALLLDALLLPDVSLPQRSIIKGDALCLSIAAASIIAKVTRDRLMLQLHEQYPL
                     YGFARHKGYGTEAHLAALREYGASPVHRQSFAP"
     gene            complement(734..1063)
                     /gene="rplS"
                     /locus_tag="VGT44_20450"
                     /pseudo
     CDS             complement(734..1063)
                     /gene="rplS"
                     /locus_tag="VGT44_20450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004415194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(1143..1409)
                     /gene="rpsT"
                     /locus_tag="VGT44_20455"
     CDS             complement(1143..1409)
                     /gene="rpsT"
                     /locus_tag="VGT44_20455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV2583244.1"
                     /translation="MPNNAAAKKRMRQETKRRLHNRSVKSLVKTQVTKARVAITSGQD
                     SEEAVRAAVSELDRAAKKGVIHRNNAARRKSRLMKKLNAANATR"
     gene            complement(1555..>2522)
                     /locus_tag="VGT44_20460"
     CDS             complement(1555..>2522)
                     /locus_tag="VGT44_20460"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEV2583245.1"
                     /translation="AIPYVGAVKLQQFSNDVNLLKQYGANYTPFQQKLTSAQQALKNA
                     KSVSDFLKFSAQIDNDTSTIQFPLAQGQANYLLKKFHAEVTSWGKTHQYHDAYDGGVY
                     NLDYEYDQQGIGSDADAAVQSSQTLDDYQSAIDLLNNDFINLHAMEADYGDPTAWNHW
                     HSTDVSLMQHYGVFGANSGPVMVVSLIEQTLRYYNNGKLVRAFQVVTGQYLKPSPPGF
                     WSIFLREHPTVFKSSEPPGSAFWYPPTPIQYAMEYHSGGYFFHDSWWRGVYGQGMNFP
                     HGDPTSAYGTTTFNDNGSHGCVNMNPNDVAWLYTQIPWGTPVIMY"
BASE COUNT          477 a          674 c          798 g          573 t
ORIGIN      
        1 gggggcgaag ctctggcggt gaacgggtga cgcgccgtat tcgcgcaagg cggccaggtg
       61 ggcttcggtg ccatagcctt tgtgccgggc gaagccgtag agggggtatt gctcgtgcag
      121 ttgcagcatc aagcggtcac gggtaacttt ggcgatgatg gaagcggcgg cgatggagag
      181 gcagagtgcg tcacctttga tgatggagcg ctgcggcagc gacacgtcgg gcaagagcag
      241 ggcatccaac agcagagcgt gtggcgcagg cgtgagttgg tgaaatgctg aacgcatggc
      301 gtgtttggtg gcctgcaaaa tgttgcgctc atcgatcagt tcaacggagc cgatgccgac
      361 ggcgaccgcg accgcgctgc gcgtgataac gtcgtagagg ttctcccgcg cctgggcgga
      421 caactgcttt gagtcgcaca cgccggcgaa gcgttccgcg caggtctcgt cgtcgagcgg
      481 caggatgaca gccgccgcta ccaccggtcc ggcgaggcag ccgcgccctg cttcgtccag
      541 cccggcgaca aaggaatagc cctgacgaag cagggccatt tcttcctcaa gggtcggttg
      601 tgatctactc gttggtgctt tcaacgggcg cagtttccgc gatgttgggg gtactgacag
      661 cggcggtcgc tacagccgcg gcgcgagcgg ccctggcggc ttccttcgcc gccgcctctt
      721 tggaggtggt cgggcgaatt tccttcaagc gagcggcttt gccggtcaag ccacgcaggt
      781 agtagagctg cttgcggcgt acgcgcgcgt ggcgaacgat ttcgattttg tcgatacgcg
      841 gtgagttcag gagaaatgta cgttcgacac cgacgccatg tgtaatgcgg cgcacggtaa
      901 agttgcgatt aaccccgccc ttgcgcaggc gcatcaccac accctcgaag ggctgaagac
      961 gctcgcggct tccttcaacg accttgactt gcaggcgtac cgtatcgccc gacttgattt
     1021 ccggtatatc ttttcgaata tgagggcgct ccacgacggc catcagcgct ctcccggcag
     1081 gtttggtaat aacttctttc tctgccatcc tggttcaatc tgcctttccc gactagcgga
     1141 atctacctgg ttgcgttcgc cgcgttgagc ttcttcatca ggcgcgactt gcggcgagcg
     1201 gcgttattgc gatgaatgac gccttttttg gcggcgcgat ctagctcgct gacggcggcg
     1261 cggacggcct cttcagaatc ctggccgcta gtgatagcga cgcgagcttt ggtgacctgc
     1321 gttttcacga gtgatttaac gctgcgatta tgcaagcggc gcttcgtttc ctggcgcata
     1381 cgcttctttg cagctgcatt attgggcatg tgagtggtaa acctccccga atcaaccgcc
     1441 ccatcacatg ggcgagttta caaagatgcc gacgcttgtg tcggactgag tgcctacgga
     1501 ttatagcaga agttcgatag acctgtcaaa ccttttccga cggtagagtt catgttagta
     1561 cataatcacc ggcgttcccc agggaatctg ggtatagagc caggcgacat cgttggggtt
     1621 catgttgaca caaccgtgac tgccattgtc gttgaaggtg gtcgtgccat aggcggacgt
     1681 cgggtcgcca tgcgggaagt tcatgccctg gccatagacg cctctccacc agctatcgtg
     1741 gaagaagtaa ccacctgaat ggtattccat ggcatactgg atcggcgtgg gcggatacca
     1801 gaaggccgaa ccaggcggct cgctcgactt gaagacagtt ggatgctcgc gcaggaagat
     1861 gctccaaaat cctggcggtg atggcttcag gtactggccg gtgacgacct ggaaggccct
     1921 gacgagcttg ccgttgttat agtagcgcag ggtttgctcg atcagtgaga ccaccatcac
     1981 cgggccggaa ttcgcgccaa agacgccata atgctgcatg aggctgacgt cggtagaatg
     2041 ccaatgattc caggcggtcg ggtcgccgta atcggcctcc atcgcgtgga gattgataaa
     2101 gtcgttattc aacaggtcga tggctgactg gtaatcatcc agcgtttggg acgactgcac
     2161 ggctgcatcg gcgtccgaac cgatgccctg ctggtcgtat tcgtaatcca ggttatagac
     2221 accgccgtcg taggcatcgt gatactggtg ggttttgccc cagcttgtga cctcggcgtg
     2281 gaactttttg agcaggtagt tggcctgacc ctgcgccagt gggaactgga ttgtgctggt
     2341 gtcgttatcg atctgagcgg agaatttcaa gaaatcgctg acagactttg catttttgag
     2401 cgcctgctga gcgctggtca acttttgctg gaaaggcgtg tagttggcgc cgtattgctt
     2461 aagcaggttg acatcgttgc tgaactgctg gagcttgacc gcgccgacgt agggtattgc
     2521 ct
//
LOCUS       DASZAK010001153         3255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_29590, whole genome shotgun sequence.
ACCESSION   DASZAK010001153 DASZAK010000000
VERSION     DASZAK010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29590"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(363..1892)
                     /locus_tag="VGT44_20465"
     CDS             complement(363..1892)
                     /locus_tag="VGT44_20465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEV2583246.1"
                     /translation="MYIPLQLRLTLFYGLLLGLALLVFGNVVYAQAEQRAYSDLDNAL
                     SSRAASVQIGKDLLAQQNPARLPMILSDSINGLEGGDIAIQVLDSHLNLLATTTFDTT
                     PDPGLTSVSGFTTSPVPWDKQAARQVLAHPVDSSGNTVAIYTTITYQGQHVRVYTTIN
                     PNFGLDHLIQTAQSESAIEQSLNNLRVLLWSGGALVMALALLGGWLLTWGVLATTRRI
                     SRTAQAISTSQDFSQRVPEQTRLGKNELTLLASTFNTMLGNLEQVYQQQQRFVADASH
                     ELRAPITSIRCNLDLLAKAPDLAPAEVAAALSETRAEADRMGRLVNELLALARADNAK
                     QEARNNDYKKPDGYLNAVDLDSLLLEVFRSYRSAYEQGQEMHIASGPRIVLQDITPAQ
                     TRGDADKLRQALVALLDNAVKYTPSEGSVALSLTVEDTRALIRISDTGIGILPEDLPH
                     IFERFYRADRARSRDRGGSGLGLAIARSIVEEHGGSIAAESTPGKGSTFVVRLPLHIQ
                     K"
     gene            complement(2086..2805)
                     /locus_tag="VGT44_20470"
     CDS             complement(2086..2805)
                     /locus_tag="VGT44_20470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583247.1"
                     /translation="MERETSPGAHILVVDDDSRVISALRRTLAYEGYQVSAAVNGEQA
                     LFLARTKRPDLVILDRMLPGIDGLEVCQRLRAAAANESGGDVAILMLTARDAVADRVA
                     GLETGADDYLVKPFALEELLARVRALLRRRQPPDIVRETLVYEDLELDTATRQARRGS
                     RLINLSTTEYELLALFMRNPRIVLTRALLMDRIWGNDFEGGPNVLEVYIGHLRARLEC
                     NGEKRLIQTMRGTGYVLRANE"
     gene            complement(2818..>3255)
                     /locus_tag="VGT44_20475"
     CDS             complement(2818..>3255)
                     /locus_tag="VGT44_20475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583248.1"
                     /translation="YAANGALGIVTEISLKNGGTSNVFSDQISATGHFDPAISSLTQE
                     QKTRVLRNSAVLSPDQSILYFAGVDGIWSVKTSDLGARHPTFTHLLQGQTFTGIALSA
                     DSQTLYAVDPAHGITMMDVATGQAEQVIQGPARAPWDIDWIYG"
BASE COUNT          611 a          944 c          985 g          715 t
ORIGIN      
        1 aacatatccc aactgggcct gggaagcaga aaggtcaaca tattggcgag acccgccaga
       61 cccgtcatca tgcctaaaat cagacgtatt cgtctctgct tgagcaatgg tagggattcc
      121 ataggtggga gcgttttgtt ccacatagag acattctttc ttttaattgc ataggagtta
      181 tcacatattc tttgaatgct ttacaaaaca gatttcatga aattcctcca atgaatctcc
      241 ataaagtgta cggtggtgag catcagaatt catgtacctc ttttctatgg aggtgacggt
      301 gggaaccgcc tggaagttcc tggaaatggg tgtgaataat tcacaaaatt ggtgtgaacc
      361 tgtcactttt ggatgtggag cggcaatcgc actacgaacg tgcttccttt accgggcgta
      421 ctctccgctg caatcgagcc gccatgctct tcgacgatac tgcgagctat ggccaggccc
      481 aggccgctgc cgccgcggtc gcgcgatcgc gcccggtcgg cgcgatagaa ccgctcgaag
      541 atatggggca ggtcttcggg caatatcccg atgcccgtgt cgctgatgcg tatgagcgcg
      601 cgcgtatcct cgacggtcag agaaagcgcg accgatccct ctgatggcgt atatttcacg
      661 gcgttatcga gtaaggccac cagcgcctgg cgtagcttgt cggcgtctcc gcgcgtctgg
      721 gcgggcgtga tatcctgcaa cacgatgcgc ggcccgctgg ctatgtgcat ttcctgtccc
      781 tgttcgtagg cggaacggta ggagcgaaag acttccagta gcaggctatc cagatcaacg
      841 gcattgagat atccgtcagg cttcttatag tcgttgtttc tcgcctcttg tttcgcatta
      901 tcggcgcgcg ccagtgccag cagttcgttg accagtcggc ccatgcggtc ggcttcggcg
      961 cgggtctcgc tcagcgcggc ggcgacctcg gcgggggcga gatcgggcgc ttttgccagc
     1021 aagtcgagat tgcagcgaat gctggtaatc ggcgcgcgca gttcgtgcga ggcatcggcc
     1081 acgaaacgct gctgctgctg gtagacctgt tccaggttac cgagcatggt attgaaagtg
     1141 ctggcaagca gggtcagttc attcttgccg aggcgcgttt gttccggtac gcgctggctg
     1201 aaatcctgcg atgtgctgat ggcctgcgcg gttcgagaga tgcgccgcgt cgtcgccagt
     1261 acgccccagg tgagcagcca gccgccgagc aacgccaacg ccatcaccag cgcgcccccg
     1321 ctccatagca ggacgcgcag gttgttcaac gattgctcga tggcgctttc cgattgcgcc
     1381 gtttgaatga gatgatccag gccaaagttt gggttaatag tggtgtagac gcgcacatgc
     1441 tgaccctggt aggtgatcgt agtgtagata gcgacggtat tccctgagct gtcaacggga
     1501 tgtgccagca cctgccgcgc ggcctgtttg tcccagggaa caggactggt ggtgaagcca
     1561 ctgacgctgg tcagccctgg gtcaggagtg gtgtcaaagg tggtagtcgc gagcagattc
     1621 agatgggaat cgagtacctg aatagcgata tcgccccctt caagcccatt gatgctgtcg
     1681 ctcagaatca tgggaaggcg ggcagggttt tgttgggcca gcaaatcttt gccgatctgg
     1741 acactggcgg ctctgctgct cagcgcgtta tccaggtcgc tgtaggcgcg ctgctccgcc
     1801 tgggcataca cgacattgcc gaataccagg agggccagcc ccaacaatag accataaaac
     1861 agcgtcagac ggagttgaag aggaatatac atcgtgcggt ccctccttct ctgatcagga
     1921 tgagcgtttt tgccagggcg accgcgaggg tcgcctctcc cagcagagcc tgtcatcctg
     1981 agcgcagcga aggatctcgg taccctgcga gtcagaccct tcgctgcgct cagggtgaca
     2041 ctcgacggca gggtgcctgg cggtcgccct ggcatcatct ccctcctact cattcgccct
     2101 cagcacgtag ccggtgccgc gcatggtctg gatcaagcgt ttctcgccgt tgcattccaa
     2161 cctggcgcgc aggtggccaa tatagacctc cagcacgttc gggccgccct cgaagtcgtt
     2221 accccagatg cgatccatca ggagggcgcg tgtcaggacg atgcgcgggt tgcgcatgaa
     2281 gagcgccagc agttcgtatt cggtggtgct gaggttaatc aggcggctgc ctcgcctggc
     2341 ctggcgtgtg gcagtatcca gctccagatc ttcgtaaacc agcgtctcgc ggacgatgtc
     2401 gggcggctgt cgcctacgca gcaaagctct cactcgcgcc agtagttctt ccagcgcgaa
     2461 gggcttcacc aggtaatcgt ccgcgccggt ctccagccct gctacccggt cggccaccgc
     2521 gtcgcgcgcc gtcagcatca agatggccac atccccgccg ctctcgttgg ccgcggcggc
     2581 gcgcagtcgc tggcagacct ccagcccatc gatgcccggc agcattcggt cgaggatgac
     2641 caggtcaggc cgtttggtgc gcgccaggaa cagcgcctgc tcgccattca cggcggcgga
     2701 gacctggtag ccctcgtatg ctaacgtgcg gcgcagggcg ctgattacgc ggctgtcatc
     2761 atcgacgacc aggatatgcg cgcctggcga cgtttctctc tccatactat gctctgttca
     2821 accgtaaatc caatctatat cccagggtgc gcgcgcaggt ccctggatga cctgttccgc
     2881 ctggcccgtt gcgacatcca tcatcgtgat gccatgcgcg ggatcgacag cgtacaacgt
     2941 ctggctatcc gcgctcaagg cgatgccggt gaaggtttgc ccttgcaaca ggtgtgtgaa
     3001 cgtgggatgc cgtgcgccga ggtcagaggt ctttaccgac cagatgccgt ccacgcctgc
     3061 gaaatagagt atcgattggt cgggtgaaag taccgcgctg ttgcgcagta cgcgcgtctt
     3121 ttgctcctgg gtcagactgc tgatcgccgg gtcaaaatgc ccggttgcgg agatctggtc
     3181 gctgaagacg ttgctggttc ctccattttt caagctgatt tcggtgacga tccccagcgc
     3241 gccgttggcc gcgta
//
LOCUS       DASZAK010001154         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_64328, whole genome shotgun sequence.
ACCESSION   DASZAK010001154 DASZAK010000000
VERSION     DASZAK010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64328"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2212)
                     /locus_tag="VGT44_20480"
     CDS             complement(<1..2212)
                     /locus_tag="VGT44_20480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEV2583249.1"
                     /translation="MVEIVQPPRRLEHTPLFQVMFAWQNNQQGTFELPDLNVEPAGIP
                     FDQIKFDLGLMLREAEGTIVGRLSYAKTLFDAATIERQSCYLLALLRAMVADAQQSVA
                     RIDVLPSSERDLLLETWNRTETTYPAHLCIHQLFEQQVRQTPEAIALVFEDQALTYAE
                     LNVQANRLAHLLIKNGVKPDERVAICVERSPTMVVGLLAILKAGSAYVPLDPSYPTER
                     LIQLLNDAEPVLLLADSTGQQALGEAAIAHLHTIDLDQLSSTCALFPTNNPDPQTLGL
                     TSRHLAYVIYTSGSTGTPKGVMVEHAQVVNFLRWSSQVFEPLETVRTLFSTSINFDLS
                     VYECFVPLIQGTTLHLVDDVLTLMRIPQEISLINTVPSAIRSLLDAQAVPLSVHTVNL
                     AGEPLKAALIRNIFVSTQTQRVCNLYGPSETTTYSTWMQMSRDDLIIESIGRPIANTR
                     IYLLDNYGQPVPLGAVGEIHIGGAGVARGYLNRPELTAERFLLDPFSSVPGARMYKTG
                     DLARYLPDGNLEFLGRNDQQVKIRGFRIELGEIEARLAEHPTVHEATVVAREDTPGDK
                     RLVAYVVAAENAESDLAAILRTHLSARLPEYMVPAAYVRLDALPLTPNGKLDRKALPA
                     PEGDAYARQAYAPPQGEIEQTLATLWAELLGIERISRHDNFFELGGHSLLAVQLMERL
                     RRLGLGAEVRTLFATPTLAELAAILGSYRDVYIPPNAITPHSTAITPDMLPLIDL"
BASE COUNT          486 a          567 c          732 g          475 t
ORIGIN      
        1 gtagatcgat cagcggcagc atgtccggag tgatggcggt gctatgtggc gtgatcgcat
       61 taggtggtat atagacgtcg cgatagctgc ctaagatggc ggccagctcg gccagagtag
      121 gagtggcgaa gagggtacgc acctcggcac ccagaccaag gcggcgcagc cgttccatca
      181 gctggacggc gagcagggag tggccaccga gttcgaagaa gttgtcgtga cgactgatgc
      241 gctcgatgcc gagcagttca gcccagaggg tagccaaggt ctgctcgatc tcgccttgcg
      301 gtggcgcgta ggcttgtcgc gcataagcat cgccctcagg agcgggcagg gccttgcggt
      361 cgagcttgcc attgggagtc agcggcaggg catcgagtcg tacataggca gcgggcacca
      421 tgtactcggg cagacgagca ctgaggtggg tacgcaagat cgcggcaagg tccgactcgg
      481 cattctcagc ggccaccaca taagccacca gacgtttatc acctggcgtg tcttcgcggg
      541 cgacaaccgt ggcttcatga acggtcggat gttcggccag acgggcctcg atctcgccta
      601 gctcgatgcg gaagccgcgg atcttgacct gctgatcgtt gcgaccgagg aactccagat
      661 tgccatcggg caggtagcga gccaggtcac ctgttttata catgcgtgcg ccgggcacgg
      721 agctgaaggg atcgagaagg aagcgctcgg cggtcagttc gggacggttg agatagccgc
      781 gggccacacc ggcgccgccg atatggatct caccgacagc gcccagtgga actggttggc
      841 catagttgtc cagtagatag atgcgcgtgt tcgcaatcgg tcggcctatg ctctcaataa
      901 ttagatcatc tcgggacatc tgcatccacg ttgagtacgt ggttgtctcg gaggggccgt
      961 aaagattgca aacccgctga gtttgagtgc ttacaaatat atttctgatt aaagcggcct
     1021 tcaacggctc accggccaaa tttacggtgt gaactgacaa cggtactgcc tgagcgtcaa
     1081 gcaaggatcg gatcgcagaa ggcaccgtat tgatgagaga gatctcttgc ggtattcgca
     1141 tcagtgtcaa gacgtcatct acgagatgca gggtggtgcc ttgtataagc ggaacaaaac
     1201 attcgtagac ggatagatcg aaattgatcg atgttgagaa cagcgtccgc acggtctcca
     1261 atggttcaaa aacctggcta ctccagcgca gaaaattgac gacttgggca tgctcgacca
     1321 taactccctt gggagtgccg gtagagccgg aggtatagat gacataggcc agatgacggg
     1381 aagtcaggcc caaggtttgt gggtcgggat tgtttgtggg gaacagggcg caggttgacg
     1441 acaactggtc gaggtcgatg gtgtgcagat gtgcaatggc tgcttcgccg agggcctgtt
     1501 ggcctgtgct gtcggcgagc aggagcaccg gctctgcatc gttcagcagt tggatcagac
     1561 gttcggtagg atagctaggg tcgagtggga cataggcgct gccggctttg agaatggcca
     1621 gcagacccac gaccatggtt ggactgcgtt cgacgcagat cgcgacacgc tcgtcaggct
     1681 tgacgccgtt ttttatcagc aggtgagcca gacgattggc ctggacgtta agttcagcat
     1741 aggtcagagc ctggtcttca aagaccaagg cgattgcctc gggggtctga cgtacttgtt
     1801 gttcgaacaa ctgatgaatg cacaagtggg ccggataggt cgtctcggtg cgattccatg
     1861 tttccagcag caggtcacgt tcgctggaag ggagtacatc gatgcgcgcg acggattgct
     1921 gtgcgtcggc gaccatggcg cgcagcagcg cgagcaggta acaactttga cgctcaatgg
     1981 tggcggcatc gaaaagagtc tttgcgtaac tcagccgccc aacgatagtg ccttcagcct
     2041 cccgcagcat gagccccaaa tcgaacttga tctgatcgaa gggtattcct gcaggttcaa
     2101 cgttcaggtc aggaagttcg aaggttcctt gctggttgtt ctgccaggcg aacatgactt
     2161 ggaacagtgg ggtgtgctcc agccgcctgg gcggttggac gatctctacc acctgctcga
     2221 agggcaggtc ttgatgatct tgggcagtca atgcggtatg
//
LOCUS       DASZAK010001155         3275 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_64364, whole genome shotgun sequence.
ACCESSION   DASZAK010001155 DASZAK010000000
VERSION     DASZAK010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64364"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="VGT44_20485"
     CDS             <1..380
                     /locus_tag="VGT44_20485"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEV2583250.1"
                     /translation="LHNSPRTLHCYIFAPLEQRIANVMEHQHLNQEEASEMVAHRDYE
                     HEAYLRRYYGTHQERPELYHLLINTGLFPFDLAANLIRETLPLVGAFSNAGTSPIPSI
                     DEADRTETLGAIATAETTETEES"
     gene            complement(493..756)
                     /locus_tag="VGT44_20490"
     CDS             complement(493..756)
                     /locus_tag="VGT44_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583251.1"
                     /translation="MKRTFFIIAIVLAALCVVLGVYYSIPGVPHPFFVAGYGKIIFVN
                     PLTQPIAARYTHHKYTAAFLVLALLLGGVAFFSRGRKSVVNVA"
     gene            complement(774..1388)
                     /locus_tag="VGT44_20495"
     CDS             complement(774..1388)
                     /locus_tag="VGT44_20495"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2583252.1"
                     /translation="MQDEEQPQAQEQTIPDMVDDVRAGKMNRRQLIKRLTIMGVSAAG
                     AGAIAAVAAHQFSSNAAAPQNNGDNTNAQQQIDQHDQHIAHQSTGNIQQLHQDYHENA
                     IVEDSMYPAPFVGRQAIMARKSAGMAAIPNVQINVTKRIVHGNTLTVEWTASGTHVHD
                     YPGLPATGRTFSIPGVTVVVRQNGKIMRESLYYDMAEVRRQFGL"
     gene            1703..2293
                     /locus_tag="VGT44_20500"
     CDS             1703..2293
                     /locus_tag="VGT44_20500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583253.1"
                     /translation="MQQTNTSPLSQKPLPFALISLLGGAAVLLGLGLIIFAHYHERAY
                     VLLGLGAAGLIGGIAGAVGAGSKVKTALGYGLIALGIVGVAVGVNYLVDVYGYFTSRH
                     YGEIVLILSAIALVAGIAAALFANPRGGIATLSSIVTLGVIASSGVVGLILGAIYLVI
                     YERQAHAYSLMAAGALCLVGGIACGIIAQRKAKLAV"
     gene            complement(2342..2977)
                     /locus_tag="VGT44_20505"
     CDS             complement(2342..2977)
                     /locus_tag="VGT44_20505"
                     /inference="COORDINATES: protein motif:HMM:NF017007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HEV2583254.1"
                     /translation="MDNYPRYDQREPQQQQQQAQWQAPQSHQWQPEWQAPPYTVPPQY
                     TPYAGQVPVMVMEPQKSWLATVLLCQFLGTLGVHRFYTGRIVSGIFQLLTFGGFGIWT
                     FIDLIMIVSGEFKDQYNRPLDQPPVMGGSRSWVTTAFLCMFLGWLGVHRFYTGHVVSG
                     IFQLLTFGGFGIWTLIDLIMIYTDSFKDDMGLLLTRMERAGSNVYPPQVKD"
     gene            complement(3050..>3275)
                     /locus_tag="VGT44_20510"
     CDS             complement(3050..>3275)
                     /locus_tag="VGT44_20510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583255.1"
                     /translation="LGVLLVIDGLAWMLYLSPPLANQLFSLIAVGSGLAEIPLMLWLL
                     IVGVNAQRWKEQASAAEERDLSVSGQAAVL"
BASE COUNT          716 a          964 c          846 g          749 t
ORIGIN      
        1 tgctgcataa ctcaccgcgc acactgcatt gctacatttt cgcgcccctg gagcagcgca
       61 tcgcaaacgt gatggaacac cagcatctca accaggaaga ggcatcagag atggtggcgc
      121 accgcgatta cgagcatgaa gcctacctgc gccgctacta cggcactcac caggaacgcc
      181 ccgaactcta tcacctgctc atcaacacgg gcctgttccc cttcgatctc gcggccaact
      241 tgatccgcga gacgctgccc ctggtaggcg cgttcagcaa cgcgggaaca tcccctatcc
      301 catccatcga cgaggccgat cgaacagaaa ccctgggcgc gatagctact gcggaaacca
      361 cagaaacaga ggaaagttga tcttgcgcgc attgttctgt ttatgtatac tggttacagg
      421 gcgctggcgt cccctgaccc ggccaaccaa caatggcgtt ggaaggtaag ggtcgggtgc
      481 ccacccatca ttctatgcta cgttgacgac agacttcctc ccacggctga agaaagcgac
      541 tcctcccagc agcagcgcca gcacaagaaa cgcagccgta tacttatgat gcgtgtacct
      601 cgccgctatc ggctgcgtca gcgggttgac aaagatgatc ttcccatacc cggcaacgaa
      661 gaaaggatgc gggacgccgg gtatggaata atagacgcca agcacgacac aaagcgccgc
      721 cagcacaatc gcaatgatga agaacgtcct tttcatggag accctcgcgc tgcttagagg
      781 ccgaactgcc gccgtacctc ggccatgtca tagtagagcg actcgcgcat aatcttgccg
      841 ttctggcgca ccacgaccgt cacgcccgga atcgagaagg tgcgaccggt ggcaggcagg
      901 ccggggtaat catgcacatg cgtaccggag gcagtccact ccaccgtcag cgtattgcca
      961 tgcacaatcc gtttggtgac gttgatctgc acattgggaa tagcagccat acccgcgctt
     1021 ttccgcgcca tgatggcctg gcgtccaaca aaaggcgctg gatacatgct atcttccaca
     1081 atggcgtttt catgataatc ctggtgcaat tgctggatat tgccggtgga ctggtgggcg
     1141 atatgctgat cgtgctgatc gatctgctgc tgggcgttgg tattatcccc gttgttctgt
     1201 ggcgcggcag cattcgatga aaactggtgc gctgcaaccg ctgcaatcgc gcccgctccc
     1261 gcggcggaca cacccatgat tgtcagcctt ttaatgagct ggcggcggtt cattttgccc
     1321 gcgcgcacat catctaccat atcaggaata gtctgttctt gtgcctgtgg ctgttcttcg
     1381 tcctgcatct cgtttcctct gtttcctctt aaatatacat agcactcata ccactactgt
     1441 cggccagcac gtctctcgac tgtacctcgt atgctataca gtacactacc atagcgagag
     1501 caagataccg gttacaatct tgttaagaaa gcgcaagaag ttcaaagaca cggttgcgat
     1561 ttatcaaccg tcctctatct acccactttg agaatgacca atctacctat cctggcaacc
     1621 atctccgttc ctaaatcgta tgtatctagt ggtgtgggtg gtaagatccc caattagaaa
     1681 acatatgtaa ggagttcttt ctgtgcaaca aaccaatact tcacctctct ctcaaaagcc
     1741 tctcccattt gcgcttattt ctctgcttgg cggcgcagcc gtgctgctgg gcctgggtct
     1801 gatcatcttc gcgcattatc acgaacgcgc ttatgtcttg cttggcctgg gcgcggctgg
     1861 cctgatcggc ggcattgccg gagcggtcgg agccggatcg aaagtcaaga cagccctggg
     1921 ctatggcctg atcgcgctag gaatcgtagg cgttgcggtt ggcgtcaact acctggtcga
     1981 tgtctatggc tacttcacca gccggcatta tggtgaaatc gtgctgatct tgagcgctat
     2041 tgccctcgta gcgggtatcg cagccgcgct attcgccaac cccagaggcg gcatcgccac
     2101 gctctccagc atcgtgaccc tcggtgtaat agcctcctct ggcgtcgtcg ggctgatcct
     2161 cggcgccatc tacctggtca tttacgagcg gcaggctcac gcctattcgc tgatggctgc
     2221 gggcgccctg tgcctggtcg gcggcatcgc ctgcggcatc atcgcccagc gcaaagcgaa
     2281 gctcgcggta tgaccgtcac cccattcaac ttactgtcat gagttttctc caaagtgtct
     2341 ttcaatcctt gacctgggga ggataaacgt ttgaccctgc gcgctccatc ctggttagaa
     2401 gcaggcccat gtcatccttg aagctatctg tgtagatcat tatcaggtca atcagcgtcc
     2461 agatgccaaa gcccccgaag gtgagcagtt gaaagatgcc gctcacaacg tgccccgtgt
     2521 agaagcgatg cacaccgagc caacccagaa acatgcataa aaacgccgtg gtgacccagc
     2581 ttctactccc ccccatcaca ggcggttgat cgagcggacg gttatattga tctttgaatt
     2641 caccgctgac gatcattatc aggtcaataa aggtccagat gccaaaacct ccgaaggtga
     2701 gcagttgaaa gatgccgctg acaatacgcc ccgtgtagaa gcgatgcacg ccgagggtgc
     2761 ctaaaaattg gcacagcagc acggtggcaa gccagctttt ctgcggctcc ataaccatca
     2821 ccggtacctg tcctgcatag ggagtatatt gtggcggcac cgtataaggc ggggcttgcc
     2881 attctggctg ccactgatgc gattgtggcg cttgccattg ggcctgttgc tgctgctgct
     2941 gaggctctcg ctgatcgtaa cgcggataat tgtccataat tcttgttccc tttctctttt
     3001 ccgatggatt taacattcac gtgagtcacc ttcgtgatgc tttcgttaat cataacactg
     3061 ccgcctgccc tgacacgcta agatcgcgtt cctctgctgc gctggcctgc tctttccatc
     3121 gttgagcatt cactccgacg atgaggagcc agagcatcag cgggatttcc gccaggccag
     3181 agccgacagc gatgagggag aacagctggt ttgcgagcgg tggtgagagg tagagcatcc
     3241 aggctaatcc atctatcacc aacagcaccc ccaga
//
LOCUS       DASZAK010001156         5767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_348822, whole genome shotgun sequence.
ACCESSION   DASZAK010001156 DASZAK010000000
VERSION     DASZAK010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5767
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348822"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="VGT44_20515"
     CDS             complement(<1..920)
                     /locus_tag="VGT44_20515"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2583256.1"
                     /translation="MTTNALDFPSWFSDGCYIGLVCCMVAIMVNALHTAWRKRGTTRQ
                     LATSLVCCVTSALLLLPAILWFNVRFSSLQASASALEIALMLVYVSLWGLVVPFSSTA
                     VFCLYTQPRDSNTSFRIPRPRSKRTTVGNAASIPAASAAGVPANLPHRLPDRPAPFVY
                     SEDQPWGWLTHRNGRFQGQRLALKRNVITIGREEDNDIWLDDETSSRYHAELAWMNDQ
                     PYVTDCNSLNGILLNGRRMRGTLALAHGDFVEIGAHRFLFESAPRPVASNELDDPLLP
                     HRRRPLSVAPLSDMPDGADLYVSEPLPVPPR"
     gene            complement(933..2021)
                     /locus_tag="VGT44_20520"
     CDS             complement(933..2021)
                     /locus_tag="VGT44_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583257.1"
                     /translation="MGKKMTILLAMLVVVTAISGGFVVGGIQAYSRFQQSSLAMQEQC
                     GGQPPVHICVQAPTEVFSAYYPFYLSTHTAQFFVSYSSDSLLTLIINASVVGFTQLET
                     HTVNATTVTQQKGFIPTLLNSAVRSQVADTHTSLSVRVTDTRGKLYYTNDSPLLLHSH
                     RLMQWVAANRLKIAAWVTPDDPAVKSLVAKAASLLPNEPGPAPVAMLGYANHATARAV
                     RDQVDAIFDAMSQDYHMHYSQAPVPYGGPGDTNISLQNIDLPAETLAQRSGMCIELTV
                     LLASAVEQIGLHAEIVIIPGHAFLGVATTPILTRNTAYEYWDAVDVNSNVAGDSANIY
                     TDQRYKTYQIVDTIAISDARQAGVEPMV"
     gene            complement(2077..3243)
                     /locus_tag="VGT44_20525"
     CDS             complement(2077..3243)
                     /locus_tag="VGT44_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HEV2583258.1"
                     /translation="MSTAAPRFTLGIEEEFQLVDRQTGQLSSSILTLLEKGAAQFGDK
                     IKAEMLQSTIELITDVCPTLADARLELLRLHTALLQLVEQEGLTLISAGTHPSDSWLK
                     QLSTPNPRYLELEEQMQDVVRSLLIFGLHVHVGVESFELAIALMNQLRTWIPHFLALS
                     SNSPFWEGRLTGMKCYRPVVWRHLPRNGVSEPVASWREFEDYVQSLTQAGFIDNAKKI
                     WWDIRPHPFFKTIEFRVFDMPATLDDTLAIAALCQALVARLSWMYARGMSMPILPRHF
                     IDENKWRAMRYGLDADIFDFAHGRAMHMRDDIGELLAFIDDVVDDLGSRREIDYLRAL
                     LANPRGTGADRQVAVYQQTGSVQSVTRFLMEQTLQGFALAIPTSPIALSARETG"
     gene            complement(3800..5557)
                     /gene="polX"
                     /locus_tag="VGT44_20530"
     CDS             complement(3800..5557)
                     /gene="polX"
                     /locus_tag="VGT44_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEV2583259.1"
                     /translation="MDKNQVAQTLEEVASLLELKEGSNPFETRAYENAARAVSALDGD
                     IEQLTREGRLKGTPGLGSTIIKRIEELVNTGHIALLDELIAETPQIKLEMRRIPGVGP
                     KKINAIVNQLHVTTIAELEQACNDDRVAKLPGFGKKTQDNIMQGLAFLAQHEGRFLFP
                     VAEDEAERMRLVLADIPTIVRLQVGGSLRRRRETIGDIDMVASVADDAGEETRREIMN
                     VFTGQPGVQAITGKGETKSSVVLKSGINMDLRVVNDSQFPATLHHFTGSKEHHIPLRR
                     RALSMGMTINDYGLFRGKEPHLELVPCNEEADIYTALGMAYIEPELREDTGEIEAAIQ
                     GTLPTLVQMSDLRGILHCHSTWSDGQNTIREMAEATIARGYHYLGITDHSKVAAYAGG
                     LNEDALRRQGEEIDRLNEDFKGRLHILKGTECDILRDGSLDYADDVLASLDFVVASIH
                     SLFNLPKEEQTRRMLRAIANPYVDIIGHPTGRILLGRDGYELDIEAIIDAAAEHGVCI
                     EINAHPSRLDLDWRYLRRARDRGIKIPVNPDAHTTDGLDVIRYGINIARKGWLRASDV
                     LNTMSTEEITAYFRKRRHA"
BASE COUNT         1173 a         1619 c         1780 g         1195 t
ORIGIN      
        1 cggggaggaa ccggaagggg ctcgctgacg tacaggtcgg ctccatcggg catatcggac
       61 aagggcgcga cgctcaaagg acgcctccgg tgcggcagca ggggatcgtc cagttcgttt
      121 gatgcaacgg ggcgcggcgc agactcgaaa aggaagcggt gagcgccgat ttccacgaag
      181 tcaccgtgcg cgagggcaag cgtaccgcgc atgcgccgac cattcagcaa gatgccgttc
      241 aggctgttgc agtcggtgac gtagggctga tcgttcatcc aggccagttc ggcatggtag
      301 cgcgacgagg tttcgtcgtc cagccagata tcgttatctt cttcgcgtcc gatggtaata
      361 acgttgcgtt tcaaggccag ccgctgcccc tgaaaacgac cgttgcgatg tgtgagccag
      421 ccccagggtt gatcctcgct gtacacaaat ggagcaggcc gatccggcag gcggtgcggc
      481 agattcgcag gcacaccggc agcagatgca gcgggaatgc tggcggcatt gccgaccgtt
      541 gtgcgctttg agcgcgggcg tggaatgcga aacgaggtat tggagtcgcg cggctgcgta
      601 tagagacaga agaccgccgt tgagctgaaa ggaacgacaa gtccccacag gctaacatac
      661 accagcatga gcgcgatctc cagcgcactg gcactggcct gcagcgagct gaaacgcacg
      721 ttgaaccaga ggatggcggg gagcaagagc agcgccgagg tgacgcagca gacgagcgag
      781 gtggcgagct ggcgcgttgt gccgcgcttg cgccaggcgg tgtgcagcgc gttgaccatg
      841 atagcgacca tgcaacagac cagcccgata tagcagccat cgctaaacca gctcggaaaa
      901 tcaagtgcgt tcgtcgtcat ctccctctct cactaaacca tcggctcgac acccgcctgc
      961 cgggcgtcgc taatcgcaat cgtatcgaca atctgatagg tcttatacct ctggtcggta
     1021 tagatattgg ccgaatcgcc cgcgacattg gaattgacat cgacggcatc ccagtattca
     1081 taggcagtgt tgcgcgtcaa aatgggcgtc gtagcgacgc caagaaaggc atggccggga
     1141 ataatcacaa tttcggcatg cagcccgatc tgttcaacgg cggacgccag cagcaccgtc
     1201 agctctatgc acatgccact gcgctgcgcc agcgtctccg ccgggagatc gatattctga
     1261 agcgagatgt ttgtatcgcc gggtccgcca tacggcacgg gagcctggct atagtgcata
     1321 tgataatcct ggctcatcgc gtcgaatata gcatctacct ggtcgcgtac cgcccgcgcg
     1381 gtggcatgat tggcgtagcc gagcatggcc acaggagcag gccccggctc gttgggcagc
     1441 aagctggcgg ccttagcgac cagcgacttc acggcggggt cgtcgggcgt gacccaggcc
     1501 gctattttca ggcggttggc agcgacccac tgcatcagcc ggtgcgaatg gagcagaagc
     1561 gggctgtcgt tcgtatagta cagcttgccg cgtgtatccg taacgcgcac actgagcgaa
     1621 gtatgcgtat ctgctacctg cgaacgcacc gccgagttga gcagcgttgg gataaaaccc
     1681 ttctgctgtg tcacggtggt ggcattgacg gtatgcgtct caagctgggt gaatccgacc
     1741 acgctggcgt taatgatgag cgtaagcagg ctatcgctgc tataagagac gaagaactgc
     1801 gctgtatggg ttgaaagata gaacggataa taggcgctga acacttcagt cggggcctgt
     1861 acacagatat gtacgggggg ctggccgccg cactgctcct gcatggcgag gctgctctgc
     1921 tggaaacggc tatacgcctg gattccgcca acgacgaaac cacccgagat cgccgtaacg
     1981 acgacgagca tcgccagcag gatcgtcatc tttttgccca tgtaatcctt gcctcatgtc
     2041 cttgcccgac cagtgtattg tacaggagaa attatatcat cctgtttcac gggcggacaa
     2101 cgcaatggga cttgtgggta tagccagggc aaaaccttgc aacgtttgtt ccatcaaaaa
     2161 gcgggtgacg gattggacac tgccggtctg ttgataaacg gcgacctggc ggtcggctcc
     2221 cgtgccacgc ggattggcaa gcaaggcgcg caggtagtcg atttcgcggc ggctgcccag
     2281 gtcgtctacc acatcatcga tgaaggcgag cagctcgccg atgtcgtcgc gcatatgcat
     2341 agcgcggcca tgcgcgaagt cgaaaatatc agcatccagg ccgtagcgca tggcgcgcca
     2401 cttgttctca tcgatgaagt gacgcggcaa gatcggcatg gacatgccgc gcgcgtacat
     2461 ccaggagagc ctggcgacca gcgcctggca gagcgcggcg atggcgagcg tgtcgtcgag
     2521 ggtggcgggc atatcgaaga cacgaaattc tatcgttttg aagaagggat gcgggcgaat
     2581 atcccaccag atctttttcg cattgtcgat gaagcctgcc tgcgtcaagg actgcacata
     2641 gtcctcaaac tcgcgccagg acgcgaccgg ctcggagaca ccgttgcgcg gaagatgccg
     2701 ccagacgacc gggcgatagc atttcatacc ggtcagccgc ccttcccaga aaggcgagtt
     2761 ggaggagagc gcgagaaaat gcggtatcca ggtgcgcaac tggttcatga gcgcgatagc
     2821 cagctcgaag ctttcgacac cgacgtggac gtgcagcccg aagatcaaaa gggagcgcac
     2881 aacatcttgc atctgctctt ccaattccag gtagcgcggg ttgggcgtgg agagctgctt
     2941 cagccatgaa tcgctgggat gcgttccggc gctgatcagc gtcaggccct cctgctcgac
     3001 cagttgcagc agcgcagtgt gcaggcggag caattcgaga cgcgcgtcag caagcgtggg
     3061 acagacatcg gtaatcagct cgatggtcga ttgcagcatc tccgccttga tcttatcgcc
     3121 gaattgcgca gcgcccttct ctaagagtgt cagaatactt gaagacagtt gacccgtttg
     3181 acgatccacc agttgaaatt cttcttcgat gccgagcgta aaacgcggcg cagccgttga
     3241 catgccgagg ctcctttacc agcaatggga ttgtcgcagt accgcgagaa gcaagagaat
     3301 gggggaaaac agtcaggcag attttgagga actatcacaa tgctaccagg acacacgaaa
     3361 atagagcggc atagatggtc atcatcgtct aagctggctc gcgctctcat gcaagctcct
     3421 tgtgagaaag cgggtcacgc tacatggcgg aaaacttctg ggaatctaag cgtacacaag
     3481 aaccaggcga tttgtcaagt gctgatagct ctgagggtgt aggaactact tgtaggcaag
     3541 caatcaagta ggaagagagc caaatccctg taggagccgg tttaccgtgc tcaaagccga
     3601 tttatcggcc agtccagcct tgcgtatcaa cgaggattcg cgccgataat gaatatttac
     3661 aaccaggatc ggcaatagtt tgtcatcctg agcgcagcga aggatctcac tcgccgagga
     3721 ccgagatcct tcgctgcgct caggatgaca aaaccggctt tggtcgttca agttcatcat
     3781 cgcgtcccta cgacgggggt catgcatggc ggcgcttacg gaaataggcg gtgatctcct
     3841 cggttgacat ggtattcagc acatcgcttg cgcgcagcca gcctttacgg gcgatattta
     3901 taccatagcg tatcacatct aatccatcgg tggtatgcgc gtcgggattg acggggattt
     3961 tgatgccccg gtcgcgggcg cggcgcaggt agcgccagtc caggtcgagc cgcgaggggt
     4021 gggcgttgat ctcgatgcag accccatgtt cggctgccgc gtcgatgatg gcttcgatat
     4081 ccagttcgta tccatccctg ccgagcagga tgcgcccggt tgggtggccg atgatatcga
     4141 cgtaggggtt ggcgatggcg cgcagcatac gccgcgtctg ctcttccttc gggaggttga
     4201 acaggctgtg gatggacgcg acgacaaagt caaggctggc cagcacgtca tccgcgtaat
     4261 cgagtgagcc gtcgcgcagg atgtcgcact cggtgccttt gaggatatgc aggcgccctt
     4321 tgaagtcctc gttcaggcga tcgatctctt cgccctggcg gcgcagcgcg tcttcgttca
     4381 gtcctccggc gtaggcggca accttgctgt ggtcggtgat gcccaggtag tggtagccgc
     4441 gcgcgatggt ggcctcggcc atttcgcgga tggtgttctg cccgtcactc caggtggagt
     4501 ggcagtgcag tatgccgcgc aggtcgctca tctgcaccaa tgtaggcagc gttccctgga
     4561 tggcggcctc gatctccccg gtgtcctcgc gcagttccgg ttcgatatag gccatgccga
     4621 gcgcggtgta gatgtcggcc tcttcattgc acggcaccag ttccaggtgc ggctctttgc
     4681 cgcgaaagag gccatagtcg ttgatggtca tgcccatgct gagggcgcgc ctgcgcagcg
     4741 ggatatggtg ctctttggag ccggtgaagt gatgcagcgt ggcaggaaac tggctatcgt
     4801 tgacgacgcg caggtccatg ttgatgccgc ttttcagtac gacgctcgat ttggtctcgc
     4861 ccttgccggt gatggcctgc actcctggtt gaccggtgaa gacgttcatg atctcgcgcc
     4921 gcgtctcctc gccggcatca tcggccacgc tggccaccat gtcgatatcc ccgatcgttt
     4981 cgcggcggcg gcgcaggctg cctccgactt gcaaacgcac gatagtgggg atgtcagcca
     5041 gcacgaggcg catgcgctcg gcctcgtcct ccgcgacggg aaagaggaaa cggccttcgt
     5101 gctgcgcgag gaaggccagc ccttgcatga tgttgtcctg cgtctttttg ccgaaaccgg
     5161 gcagcttcgc cacgcggtca tcgttgcagg cctgttccag ttccgcgatg gttgtgacat
     5221 gtaactgatt gacgatggcg ttgatcttct ttggcccgac gcctgggata cggcgcatct
     5281 ccagtttgat ctgcggtgtt tcggcgatca gctcgtccag cagcgcgata tgcccggtgt
     5341 tgaccagttc ttcgatgcgt ttgatgatgg tcgatcccag gccaggcgta cccttgagcc
     5401 ggccttcgcg cgtcaattgc tcgatgtcgc catccagcgc gctgacggct ctcgctgcgt
     5461 tctcatacgc gcgcgtctcg aaggggttgc tgccttcctt caattcaagc aggctggcta
     5521 cttcttccag cgtctgcgca acctggtttt tgtccataga tactctccat ataatttcaa
     5581 cgggctacgg cgggcgacgc aagcgtcccc actccactcc acacctctcc cgcccctacg
     5641 gagcgagttt catgtcgaag atctcgggcg gttgggctgt tacctcgaat gggttgcggc
     5701 ctttcaggtc gcggttgagg tagcgcttgc agtcgtaatg caccagcagc cagcaatgcc
     5761 attcgtt
//
LOCUS       DASZAK010001157         1873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_279303, whole genome shotgun sequence.
ACCESSION   DASZAK010001157 DASZAK010000000
VERSION     DASZAK010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279303"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..371)
                     /locus_tag="VGT44_20535"
                     /pseudo
     CDS             complement(1..371)
                     /locus_tag="VGT44_20535"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(605..1093)
                     /locus_tag="VGT44_20540"
     CDS             complement(605..1093)
                     /locus_tag="VGT44_20540"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HEV2583260.1"
                     /translation="MRNRTVLLIVGIVVFVLLATGGTVGAMALTHSGFMGNGYSNGMM
                     SGQQGYQGVMSGRYNQGGMMGGYQQNPTQQGAPVTGVTHMNMQNFAYQYANMQVKVGT
                     TVTWTNHDSAPHSVTFKNGMKDSGLLYQGQSFSYTFNTPGTYQYYCTIHPYMGATVTV
                     VS"
     gene            complement(1539..>1873)
                     /locus_tag="VGT44_20545"
     CDS             complement(1539..>1873)
                     /locus_tag="VGT44_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEV2583261.1"
                     /translation="EGDRRRGSPDMMRTSDMVPTRTIFREEETQMMYGFGDFGGWWLI
                     GMGMMVLFWVAIILLVVWVVRSLFPRQARSGRNQALETLQQRYAAGEINAAEYEQARA
                     RLEEIPVG"
BASE COUNT          358 a          576 c          516 g          423 t
ORIGIN      
        1 ggctgccgaa aggcgcagga tctcatcctc gtcgaagccg ttgttcgcca ccacattcgt
       61 cagctcgggc ttgcccttcg taatcgtccc cgtcttgtcc aggacgatgg cttgcaggcg
      121 catcgtcgtc tgcagcgcat caccggagcg gatcaggatg ccctgctccg cgcccttgcc
      181 aatgccgacg gtcagactgg tgggggtggc aagtcccagc gcgcacgggc aggcgatgat
      241 cagggtggtc acaaagacga tcagggcata tttgagcctt gtgcgtcctg caccaggcga
      301 atgatctggg ccagtgcggt atccttcccg actttggtgg cgcggaagcg caagctgccc
      361 acctggttca tggtcgcgcc gatgacctcg tcgcccgcgt gtttctcgac agggatcgac
      421 tcccctgtcg catacgctgc tgtggatagc gtgagggaaa gctgaggcta cgctgaggat
      481 tggagaaaca cgctgctatc aggcgaggtc ggggaaagca aataatcaat agccggtgga
      541 gcgcttcagc atgggtgatg ggacgcgttc cactaggcta tctggtagtc gtatggccgc
      601 ggcgctatga aacgacggtc acggtggcac ccatgtaggg gtggatcgtg cagtagtatt
      661 ggtaggtgcc aggcgtgttg aaggtgtagc tgaacgactg cccctgatag agcaggccgc
      721 tatccttcat accgttcttg aacgtcacgg aatgcggcgc gctgtcatga ttcgtccagg
      781 tgacagttgt gccgaccttc acttgcatgt tcgcgtactg gtacgcgaag ttctgcatgt
      841 tcatgtgggt cacgcctgtg accggagcgc cttgttgggt ggggttttgc tgatacccac
      901 ccatcatacc gccctgattg taccgcccgc tcatcacgcc ctggtagcct tgctgcccgc
      961 tcatcatgcc attgctatag ccgttgccca tgaacccgct gtgtgtcagc gccatggcac
     1021 caaccgtgcc gccggtcgcc agcagaacga atacaacgat accaacaatc agtagaactg
     1081 ttctattacg cattgctttc tctccttctt gattaagctt ctacctgctc tacagtactc
     1141 tctgtctctg ctcagggagc tgctctgtcg cctgaagccg ctctcgcatg tgctcaaacg
     1201 ttgcggtgtc aatttcgccg cgcgcatacc gctggcgcaa gatttctaac gcggatgggc
     1261 tggctgaccc ggatggacct gacacgtgcg ctcttctttc ccaccagcga atcaaggccc
     1321 agaccaggat cactaagatg gcaatccaca ggacaccgcc aaatgccatc atgaaccaac
     1381 cccaaccgaa accatagccc cacatcattt tcgtaccctc gctttctttc tgctttgttc
     1441 tgctaccgaa cgagcttgtc tgtcatggac gagtatgctt tactgctccg atcgctgaca
     1501 tcgctcccgg agacattctc ggatgtctcc aggcgaggtt agccgaccgg gatctcctct
     1561 aacctggcgc gcgcctgctc gtactcagcc gcgttgatct cacccgcagc atagcgctgc
     1621 tggagtgttt ccagcgcctg gttgcgcccc gaccgcgcct ggcgtgggaa caggctgcgc
     1681 actacccaga cgaccagcag gatgatagcc acccagaaga gcaccatcat gcccattcca
     1741 atcagccacc agccgccaaa atcgccaaat ccgtacatca tctgcgtctc ctcctctcgg
     1801 aagatggttc tcgtgggaac catgtcactt gttctcatca tgtcggggga acctctgcga
     1861 cggtcgcctt cgt
//
LOCUS       DASZAK010001158         3512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_705102, whole genome shotgun sequence.
ACCESSION   DASZAK010001158 DASZAK010000000
VERSION     DASZAK010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705102"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="VGT44_20550"
     CDS             complement(<1..1512)
                     /locus_tag="VGT44_20550"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583262.1"
                     /translation="MAEQLNIILVPHTHWDREWYLTFQQFRMRLVQVVNSLLDILDRD
                     PAFTHFMLDGQTIVLDDYLEACPEQEERLKRYTRTGRISVGPWYLQPDEFLVSGESLI
                     RNLHIGLSRAAEFGGAMRVGYVPDTFGHIAQLPQILRGVGLDNAVYWRGVGAEARRSE
                     FFWAAPDGTTVLVLHLADSMGYSNARDMPLAPDEFVTRTLLLASNLLPKATTSTLLFM
                     NGSDHLSPQQGLPAAIAAANQRTAHLDPDHAQALTALGHVSYDSVNVRIGSLAEYVDA
                     VRQQMQQEAGKAQTLIGEMRSSQYAHLLPSVLSTRMWIKQRNASIEHLLERRVEPLTA
                     WAALHGSPYPKGLIALAWKYLLHNHPHDSICGCSIDQVHRENRVRFDQSEQIGQQLVA
                     DATRQLVARVDTRPPATAVPGQRDRAAIPLVVFNTAPGPRSEAVQVEIPLAALNQHAV
                     IVDEQKRSMPLAVIKSGRQELGSMPLAREVIAAAVALQGTPNPGDFIRLAESTI"
     gene            1873..2832
                     /locus_tag="VGT44_20555"
     CDS             1873..2832
                     /locus_tag="VGT44_20555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583263.1"
                     /translation="MARGSSYGDPQDETPISVTSHASVPPRRTRVLPEVKDALTAHAE
                     DADHGEDDGGLEHDDEERRALVPVSDGKIQSYGYMHYTPLARHRSWISLLLICVVCVT
                     VLLGVAISAGMFQRHDGVQVVPYAGQTFSIQVGGSYNAFNLWENSNGPIPTSTAVPIP
                     THLGPYSVLGKPTITATFINQVLAYYHSPAAGMGQQMYDLGVKYGIDPVYALAFFMHE
                     SLFGTTGEARATLSLGNLRCIPTRPCIDQNRGGYAQMYSWVDGFEQWYKLIRNLYVAQ
                     WGLVTVDQIIPKYAPNSDGNNEAEYIATLKHEVDTWHAGVLTP"
     gene            complement(2822..>3512)
                     /locus_tag="VGT44_20560"
     CDS             complement(2822..>3512)
                     /locus_tag="VGT44_20560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583264.1"
                     /translation="ASSREQLVSQTPTAQAARIRILIVDNQAVTRAGLRRLLESYADL
                     QVVGEAIDGVQAVSETAELGPQVVILDAQLPQGQSLEALRQVKQLNLDTHVLLLSTLD
                     REEYLYETLRAGAEGFVLKDIAPDELAQAVRAVARGEVLVQPQIAGRLISRFGKQGHA
                     GSLLDALTPRELEVLRLLARGLRNKEIAARLFVSERTVNFHLANIYQKMNVSGRTEAL
                     SKAHEQGLITA"
BASE COUNT          689 a          942 c         1160 g          721 t
ORIGIN      
        1 gatagtactt tcggccaggc gaatgaagtc gccgggattc ggtgtgcctt gcagggcaac
       61 ggcggcggca atcacctcgc gcgcgagagg catcgaacca agctcctgcc gcccgctttt
      121 gatgacggcg aggggcattg atcgcttctg ctcatcgacg atgacggcgt gctggttcag
      181 tgcggcgagc ggtatttcaa cctgcacagc ctcgctgcgc ggacctggcg cggtattgaa
      241 gacgacgagt gggatggcgg cgcggtcgcg ttgtcctggt acggcggtcg cgggcgggcg
      301 agtatcgacg cgcgccacga gctgccgcgt ggcatcggcg acaagctgct ggccgatctg
      361 ttcgctctgg tcgaagcgca cgcggttttc gcgatgcacc tggtcaatac tacagccgca
      421 gatgctatcg tgcgggtgat tgtgcaggag atatttccag gcaagcgcta tcagtccttt
      481 gggatagggg gagccgtgca gcgcggccca ggcggtgagc ggctctaccc ggcgctccag
      541 caggtgttcg atggaggcgt tgcgctgctt gatccacatg cgcgtggaaa gcacggacgg
      601 cagcaggtgg gcgtactggc tgctgcgcat ctcgccgatg agcgtttggg ctttgcccgc
      661 ctcttgctgc atttgctgac ggacggcatc tacgtactct gccagcgagc cgatgcggac
      721 gttgacgctg tcataggaga cgtgaccgag ggcggtgagc gcctgggcgt gatccgggtc
      781 gagatgcgcc gtgcgctggt tggccgctgc gattgcggcg ggcaatccct gctggggcga
      841 gagatggtcg gacccgttca taaagagcag ggtcgaggtg gtggctttgg gcagcaggtt
      901 ggaggcgagc agtagtgtgc gggtcacaaa ttcgtccggc gcaagcggca tatcgcgggc
      961 attggaatag cccatagaat cggcgaggtg cagcaccagg acggttgtgc catcaggcgc
     1021 ggcccagaag aactcgctcc tgcgggcctc cgcgccgacg ccgcgccagt aaacggcgtt
     1081 atcgaggccg acgccgcgca ggatttgcgg caactgcgca atatggccga aagtatccgg
     1141 cacatagccg acgcgcatgg ccccgccaaa ttcggcggcc ctacttagcc cgatgtgcag
     1201 gttgcgtatc aaggattcgc cactgacgag aaattcgtcg ggctggagat accaggggcc
     1261 gacagagatg cgcccggtgc gtgtgtagcg tttcaggcgc tcttcctgtt ccgggcaggc
     1321 ttccaggtag tcatccaaca caatcgtctg cccatccagc atgaagtgag tgaaggcggg
     1381 gtcgcggtcg agtatgtcca gcaggctatt gacgacctgc accagacgca tgcgaaattg
     1441 ctggaaagtg agataccatt cgcggtccca gtgtgtgtgg ggaaccagga tgatgttgag
     1501 ctgttcggcc ataatccctc gagcttaaca tggcgaccgc aagggtcgcc actacgataa
     1561 cgttgcgtaa caggcgggtg gcatcggaat atcgtagcgg cgacccttgc ggtcgccatc
     1621 acgcactcga tgcaacctgg cagtgtgtta taattgccgg taagacaaat aagacaatcg
     1681 taccaggatg ttgaaggttg gtgttttcgc cattaccata tcatagcgtg ccaaggcgcg
     1741 tcaacatgaa gcggtgatgg gagggcgacc acaagggtac gccctcccga aagcaaagct
     1801 tgtttacaga cgagcgacgg aatgatataa tctctggtaa agtacacttg ttctagagga
     1861 ggattcctga atatggcacg gggcagtagc tatggagacc cgcaagatga gacgccgatc
     1921 tctgtaactt ctcacgccag tgttccgccg cgaaggactc gtgttttgcc agaggtgaag
     1981 gatgcgctta cggcgcatgc tgaggacgcg gatcatggcg aagatgatgg cgggttagag
     2041 catgatgatg aggagcggcg cgcgctggtc ccggtgagtg atgggaagat tcaatcgtat
     2101 gggtacatgc actatacgcc gctggcgcgc catcgctcgt ggatttcgct tttgctgatc
     2161 tgcgtggttt gcgtcacagt gctgctggga gtcgctattt cggcgggtat gtttcagcgg
     2221 catgatggcg tacaggtggt gccatacgcg gggcagacct tctcaattca ggtgggcggg
     2281 tcgtacaatg cctttaacct gtgggagaac agcaacggac caatacccac gtcaaccgcc
     2341 gttcctatac ctacgcatct ggggccatac tctgtgcttg gcaagccgac gattacggct
     2401 acttttatca accaggtgct ggcatattat cattcaccag cggcaggcat ggggcagcag
     2461 atgtatgacc tgggcgtgaa atatggcatc gacccggtgt acgcgctggc atttttcatg
     2521 cacgagagcc tgttcggcac gacgggtgag gcgcgcgcga cgctctcgct gggcaatctg
     2581 cgctgcatcc cgacacggcc atgcatcgac cagaatcgcg gcggatacgc gcagatgtat
     2641 agctgggttg atggcttcga gcagtggtac aagctgatcc gcaacctgta cgtggcgcaa
     2701 tgggggcttg tgacggtgga tcaaatcatt ccgaaatacg cgccaaatag cgacggcaat
     2761 aacgaggccg agtatattgc gacgctcaag catgaagtcg atacatggca cgcgggtgtg
     2821 cttacgccgt gatcaaaccc tgttcgtggg ctttgctgag ggcttcggtg cggcctgaga
     2881 cgttcatttt ttggtagatg ttggcgagat ggaagttgac ggtgcgttcg ctgacgaaga
     2941 ggcgcgcggc gatttctttg ttgcgcaggc cgcgtgctaa cagacgcaat acctccagtt
     3001 cgcgaggggt cagggcgtcc agcaggctcc ccgcgtgacc ctgtttgccg aaacgtgaga
     3061 tgaggcgccc ggcgatctgt ggctgtacta atacctcacc gcgagctacc gcgcgcacgg
     3121 cctgggcgag ttcgtcggga gcgatatcct tgaggacaaa gccttccgcg cccgcgcgca
     3181 gggtttcata gaggtattct tcgcgatcca gggtcgagag cagcaagaca tgcgtatcga
     3241 gattgagctg cttgacctgg cgcagcgcct ccaaactctg gccttgcggc aattgcgcgt
     3301 ccaggatgac tacttgcggt cccaactccg ccgtctcgct gacggcctgc actccatcga
     3361 tggcctcacc cactacttgc aggtcggcat agctttcgag caggcggcgc agaccagcgc
     3421 gcgtcacagc ctggttatcg acgatcagta tgcgaatgcg cgcggcctgg gctgtaggtg
     3481 tttgcgagac gagctgttcg cgcgaagacg cg
//
LOCUS       DASZAK010001159         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_313286, whole genome shotgun sequence.
ACCESSION   DASZAK010001159 DASZAK010000000
VERSION     DASZAK010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313286"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VGT44_20565"
     CDS             complement(<1..758)
                     /locus_tag="VGT44_20565"
                     /inference="COORDINATES: protein motif:HMM:NF013197.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583265.1"
                     /translation="MESIEQRIARVRDDREHGSRWLMRETITLLRDIASDTASIPGER
                     MRYLWSAGIDLVAARPAMAAIAGAVWRILGAPGDISSNTAEIDGIAHAAGQLLQTYES
                     AVTQITTHTRSLLNGTIMTHSLSGTVLDVLATCASQLDSIIVLEGRPRYEGRTMAEAL
                     SKAGLPITLITDAQAAIFLPQCAAVVLGADSILADGGILNKAGTSLLAWAARGTNKPF
                     YVLSETLKIAPMRWTANPAQLEEKEATEVLERPIP"
     gene            889..>2488
                     /locus_tag="VGT44_20570"
     CDS             889..>2488
                     /locus_tag="VGT44_20570"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV2583266.1"
                     /translation="MKQPENHGAFDQQHRQDGERPTRPIFTTPITAMIRLDRANGLPL
                     YRQICQGLREAILTGELAEGVRLPTERALARELGVNRTTVMNAYNELASEGLIEGHVG
                     RGTLVRRNHYGYNGDEYDERETASWLLGLPTGEQEALGPDARLLSELASATDRKEIIS
                     LAPGNPAPDLLPAEMLSAIFADGLINARQSALGYNPVEGLLSLRRNIAAHMRRRGVVV
                     NAPQILILSGSTQGIGLVGRFLLNPGDEVVVEVPTYLGAIQAFRALHARVIGVPTDGD
                     GMRIDLLESILARRRPRLIYTLPTFQNPTGAVMSLERRRRLLLLAKRYQTPIMEDDPY
                     GEIYFEGNPPQPLKALDTHGQVIYLSTFSKTLAPGLRVAWLVAPEPMIERLSLHKQVF
                     DLNTNAIGQWAVSEIMQRNLLDDHLERIRQVYRKKRDLTLQAIAAHWPNEVRVNHPAG
                     GFHLWCRLPGDLRARTLLREAANEQVAFTIGEPFHVDGGGQQHFRLSYAYPEEAHLDE
                     GVRRIGSAVKRLLARRSLREEAQRQ"
BASE COUNT          469 a          751 c          781 g          487 t
ORIGIN      
        1 ggtatgggcc gttccagcac ttctgttgct tccttctctt ccaattgagc cgggttggct
       61 gtccagcgca tgggagcaat tttgagggtt tcgctcagga cgtagaacgg tttgtttgtg
      121 ccgcgtgccg cccacgccag cagcgaggtc ccggctttgt tgaggatgcc gccatcagcg
      181 aggatgctgt ctgctccaag gaccaccgcc gcgcattgag gcaggaaaat agcggcctgc
      241 gcgtccgtaa tcaaggtgat gggcaatcct gctttgctca acgcctccgc catagtacgg
      301 ccttcgtagc gcggtctgcc ttccagcact attatgctgt ctagctgcga ggcacaggtt
      361 gccaggacat ccagcacggt tcctgagagg ctgtgcgtca tgattgtgcc gttgagcaaa
      421 gagcgagtat gcgttgtgat ctgcgtcacg gcgctctcgt aggtttgcag cagttgtccg
      481 gcggcgtgcg cgatgccgtc tatttcggcg gtgttgctag agatatcgcc tggcgcgccg
      541 agaatgcgcc ataccgctcc cgcaatggct gccatcgccg ggcgagcggc aaccaggtct
      601 atgcctgccg accagagata tcgcatacgt tcgccgggaa tcgaggcggt atcgctcgct
      661 atatctcgca agagcgtgat cgtctcgcgc atgagccagc gcgaaccatg ctcgcgatca
      721 tctcgcacgc gcgcgatgcg ttgttcgatg gattccatag tgtactccct tgtgagtata
      781 tgataccgca aatgctattg gctgcaagac ataccaacca atggcccctt gttcgtggtg
      841 aaaatggcgg ttatacttat attggtatga gccaatcagg agatgagaat gaaacagcca
      901 gagaaccacg gtgcatttga ccagcagcac aggcaggatg gagaacgtcc cacccggcca
      961 atcttcacaa cgccgattac cgccatgatc cgcctggacc gcgcgaatgg cttgccgctt
     1021 taccggcaaa tttgccaggg actgcgagag gccatcctta cgggagaact cgcggagggt
     1081 gtgcggctgc caaccgaacg cgcactggcg cgcgaacttg gcgtcaatcg caccacagtg
     1141 atgaatgcct acaacgagct ggcgagcgag gggctgatag agggacacgt gggacgcgga
     1201 acgctcgtgc ggcgcaatca ttatggctat aatggcgatg agtatgatga acgcgagacg
     1261 gcttcgtggc tgctcggcct gccgacgggc gaacaggagg cgctgggacc ggatgcgcgg
     1321 ctgctgagcg aactggcctc ggccactgac cgcaaggaaa tcatttctct cgcgcccggc
     1381 aatcctgccc ctgatttgct gcccgccgaa atgctcagcg ccatcttcgc ggatggcctg
     1441 atcaatgccc ggcagtctgc cctgggttat aatccagtag aggggctgct cagcctgcgg
     1501 cgcaacatcg cggcacatat gcggcggcgc ggtgtggtgg tgaacgcgcc gcaaatcctg
     1561 atcctgtccg gctcgacgca gggcattggc ctggtgggcc gcttcctgct taaccctggc
     1621 gacgaagtgg tggttgaggt gccgacctac ctgggggcta tccaggcgtt ccgcgctctc
     1681 catgcgcgcg tgatcggcgt gccgaccgat ggcgatggaa tgcgcatcga cctgctcgag
     1741 tcgatcctgg cgcgccgccg accccgcctg atctacacct tgcccacctt ccagaatccc
     1801 accggggcgg tcatgtccct cgaacgccgc cgacgcctgc tgctgctggc gaagcgctac
     1861 cagacgccaa tcatggagga tgacccgtat ggcgaaattt atttcgaggg gaatcctccc
     1921 cagccgttga aggcgctgga tacacatgga caggtgattt acctgagcac cttttcgaag
     1981 acgctcgctc cggggctgcg cgtggcctgg ctggttgcgc cggagccgat gatcgagcgg
     2041 ctgagcctgc acaagcaggt cttcgatttg aataccaacg ccatcggcca atgggctgtg
     2101 tccgagatta tgcagcgcaa cctgctcgat gaccacctgg agcgtattcg ccaggtgtac
     2161 cgcaaaaaac gcgacctgac cttgcaggcc atcgcagcgc attggcccaa cgaggtgcgc
     2221 gtcaaccacc cggcgggcgg gttccacctg tggtgccgct tgcccggcga cctgcgcgcc
     2281 cgcacgttgc tgcgcgaggc ggcgaacgag caggtggcct tcaccatcgg cgagccgttt
     2341 cacgtcgatg gcggcggcca gcagcacttc cggctaagct atgcgtatcc agaggaagcg
     2401 catcttgacg aaggcgtgcg gcgcatcggc agcgcggtca agcgcctgct ggcgcgccgc
     2461 agcctgcgcg aagaggccca gcgacagc
//
LOCUS       DASZAK010001160         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_171765, whole genome shotgun sequence.
ACCESSION   DASZAK010001160 DASZAK010000000
VERSION     DASZAK010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171765"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VGT44_20575"
     CDS             <1..610
                     /locus_tag="VGT44_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEV2583267.1"
                     /translation="DPLVAGRLFVAVEAGALLRSLDGGELWEDRKAGGPFDTHTLVMH
                     RLAPDCLYSAAGDGFMQPGNGFAQSDDGGETWYRPGEGLRHHYLWGAAADPADPSTIV
                     ISAAPGPQQAHNPTNALSALYRREGLGQWQLLRDGLPAQQGTLASVIAVNRAEAGVFY
                     VANNRGVYRSTDAGRTWEPLPIAWPAALQGNRPNALVAVSEA"
     gene            772..2070
                     /locus_tag="VGT44_20580"
     CDS             772..2070
                     /locus_tag="VGT44_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HEV2583268.1"
                     /translation="MPEIDSDHTFGFDTLSVHAGQRPDPTTGARAVPIYQTTSFVFDD
                     ADHAAHLFALQRFGNIYTRIMNPTTAAFEERIAALEGGTGSVATASGMAAQMATMMTL
                     LRPGDDVISSSSLYGGTHTQFDLTLRTWGVNTIFVDSTDPQNYRRALTPRTRAFYGET
                     IGNPRGDVLDIRAVADIAHEAGVPLIIDNTFATPYLCRPFEHGADLVVHSATKFIGGH
                     GTSIAGVLIESGRFPWDNGYFPHIVDPSPGYHGVRFYETFGDFAFVMKTRCETVRDTG
                     GCISPFNSFLLLQGLETLSLRMERHCQNAQRVAEYLQEHPAVSWVHYAGLPGDPSYQL
                     AKRYLLRGAGAIFTFGLKGGYEAGKQAINKVRLLSHLANVGDARSLIIHPASTTHQQL
                     SEEDQLAGGITPDLVRLSIGLENIDDILWDLDRALRGTSM"
BASE COUNT          433 a          668 c          655 g          430 t
ORIGIN      
        1 cgatccattg gtagcaggtc gcctgttcgt tgcggttgaa gccggagcgc tgctgcgcag
       61 cctggatggg ggtgaactct gggaggatcg caaagccgga ggtccgttcg acacacatac
      121 gctggtcatg caccggctcg cgccggactg tctctactcg gctgcgggtg atggctttat
      181 gcagccgggc aatggcttcg cgcaaagtga cgatggcggc gagacctggt atcgtccggg
      241 cgaaggtctg cgccaccact acctgtgggg cgccgcggct gatcctgccg acccctcgac
      301 cattgttatc tcggcagcgc caggccccca gcaggcacat aatccaacaa atgctctctc
      361 agcactttac cggcgcgagg gcctcggcca gtggcaattg ctcagggacg ggctgcccgc
      421 gcagcaggga acgctggcct cggtgatcgc agtcaacagg gcggaagcag gcgtctttta
      481 tgtagccaat aaccggggtg tttaccgttc gaccgacgcg ggacgcacct gggagccgct
      541 gccgattgcc tggcctgccg cgctccaggg gaaccggcca aacgcgctgg ttgcggtctc
      601 tgaggcataa attatgcaag aggctggctc agtaactcca ggctgctgag agcggaaaaa
      661 acatatccat aaccgcatgg tgtacaatgt tggggactct gtggttttgt acaaatgcct
      721 gtgtacaggt gtttgggaaa gaattgcata gagaaaaggc agtagtacgc catgccggaa
      781 atcgacagcg accatacctt tggatttgac acgctctccg tccacgcggg gcagcgaccg
      841 gacccgacga caggcgcgcg ggcggtgccg atctatcaaa cgacatcgtt cgtctttgat
      901 gatgccgacc acgccgcgca cctgttcgcc ttgcagcgtt tcggcaatat ctatacccgc
      961 atcatgaacc ccacaacggc ggcgttcgag gagcgcatcg ccgcgctgga gggcggaacg
     1021 ggatcggtcg cgacggcttc gggtatggcg gcgcagatgg caaccatgat gacgctgctg
     1081 cgcccaggcg acgatgtgat ttcatccagc agcctctatg gtgggacaca tacgcagttc
     1141 gacctgactc tgcgcacctg gggcgtcaat actattttcg tcgatagcac cgacccgcaa
     1201 aactaccggc gcgccctgac gccgcgcacg cgcgccttct acggcgagac cattggcaat
     1261 ccgcgcggcg atgttctaga tatacgcgcg gtcgccgata tcgcccacga ggcgggtgtg
     1321 ccactcatca tcgataatac cttcgctacg ccgtatctct gccgcccgtt cgagcatggg
     1381 gccgacctgg tcgtgcattc cgccaccaaa tttatcggcg ggcatggcac ctctatcgcg
     1441 ggcgtcttga tcgaatcggg cagatttccc tgggacaatg ggtacttccc gcatatcgtt
     1501 gacccttcgc caggctatca tggtgtgcgc ttttacgaaa ccttcggcga tttcgccttt
     1561 gtgatgaaaa cgcgctgcga aactgtgcgc gacacgggcg gctgcatcag ccccttcaac
     1621 tccttcctgc tgctgcaagg gttggaaacg ctctcgctgc gcatggaacg ccactgccag
     1681 aacgcgcagc gtgtggcgga atacctgcaa gaacacccgg cggtcagctg ggtgcattat
     1741 gccggtctgc ccggcgatcc ttcatatcaa ctggcgaaac gctacctcct acgcggcgct
     1801 ggcgcgatct tcacctttgg cttgaagggc ggctacgaag caggcaaaca ggcgatcaac
     1861 aaggtgcggc tcctctctca cctggcaaac gtaggtgacg cgcgcagcct gattatacat
     1921 ccagccagca caacgcacca gcagctctcc gaggaagatc agctggcagg aggcatcacc
     1981 cccgacctcg tgcgtctctc gattggcctg gaaaacatcg acgacatcct gtgggattta
     2041 gaccgcgctt tgcgtggcac atccatgtag gggccgattg atcgcgccca ccaccgctga
     2101 tgaacgttaa caatcaaagt tggacatccc gtaggcgccg gttcaccgtg cgcaccgccg
     2161 atttatcggc ctgtggagcg cgtcgg
//
LOCUS       DASZAK010001161         2409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_420756, whole genome shotgun sequence.
ACCESSION   DASZAK010001161 DASZAK010000000
VERSION     DASZAK010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2409
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420756"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VGT44_20585"
     CDS             <1..918
                     /locus_tag="VGT44_20585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2583269.1"
                     /translation="EGYRYYVQLILEGKPYHKPKHTVGTGVVGLDLGPSTIAIVPREG
                     EARLLPLCEELTPDACAKRRLQRKLDRQRRANNPQNYDDKGRIKKHGKRRLAWKNSKG
                     YLATRRRLVHQERKLAAHRRSLHGRLVHEIVRTGNTVRTEKLSYRAWQKQYGKSVGHS
                     APGMLLDQLRRTVASTGGTLIEFPTRTTKLSQYCHGCGAYAKKPLSQRWHECPCGIGP
                     VQRDLYSAWLAAHLDLPDTIPSIAHEDWEGADLRLRAAVEVLQQRAKEGQALPRSVGI
                     PRAGARLPKSLAPNQQELLYRRGRIEALR"
     gene            973..1512
                     /locus_tag="VGT44_20590"
     CDS             973..1512
                     /locus_tag="VGT44_20590"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HEV2583270.1"
                     /translation="MSSINPVVQLAQQLMLGGAVKQNHALEHATIVLLSKKYPDVRLA
                     GVSFAAGFFVFGDVPTEAILPSAQEALHLLRTTTPELAIHERCGTNLAVTGIVTSLSA
                     MTVAKMRRPYGSFNNVMLATTAALILSRPLGLTVQRYITTQTPNATMTISGVKATRFL
                     GNPAHFVSTDNPGAAGLFS"
     gene            1527..2396
                     /locus_tag="VGT44_20595"
     CDS             1527..2396
                     /locus_tag="VGT44_20595"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEV2583271.1"
                     /translation="MDRSRFLVETSWLHERLDDPRIRIVDMRGYVRTIERDGVQLAEY
                     VGARDEYVQGHIPGAVYIDWSRDIVDPDDAVEAQVASPARFAEVMSRLGIGDQHLVVA
                     YDAHPASQFATRLWWALRYYGHDEVVVLNGGLPKWQREGSPLTADVPAYPSATFTPRA
                     QPALRATAEDVLSLIGKSGVALLDARDRGQYSGKLVRGHGRAGHIPGAINIPREEVID
                     PASGLFRSNEELSRIFSQANVSPTERVVAYCNGGVAATTILFSLAMLGYPHLANYDGS
                     WNEWGSREDLPIL"
BASE COUNT          498 a          748 c          710 g          453 t
ORIGIN      
        1 gagggctatc gctattatgt acagttgatt ctagaaggca agccctacca caaaccaaag
       61 cacactgtcg gcaccggtgt cgtcgggcta gatctgggtc cctcgacgat tgccattgtg
      121 ccacgcgaag gggaggcacg gctgttgcct ctctgtgagg aactcacacc cgatgcgtgc
      181 gccaaacggc gtctccagcg caagctcgac cgccagcgcc gcgccaacaa tccacagaac
      241 tacgacgaca aaggtcgtat caagaagcac ggtaaacggc gtctcgcctg gaagaacagt
      301 aagggttatc tggcaacaag acggcgactg gttcaccagg agcgcaagct cgcggcgcat
      361 cggaggagtc tgcatggacg cctcgtgcat gagattgtcc gtactggcaa cactgttcgg
      421 acggagaagc tttcctaccg cgcctggcag aagcagtatg gcaaaagtgt gggccactcg
      481 gctccaggga tgctcttaga tcaattgaga cgcacggttg caagtaccgg cggcaccctg
      541 atcgagttcc ccacacggac gaccaagctg tcccaatact gtcacggctg cggcgcctat
      601 gccaaaaagc cgctctcgca gcgctggcac gaatgcccct gtggcattgg cccggtgcag
      661 cgcgacctgt actcggcgtg gctagcggcg catcttgatc tcccagatac tatcccctct
      721 atcgcccatg aggattggga aggtgcggac ctgcgcctgc gggcagcggt agaggtctta
      781 caacaacgcg cgaaagaggg gcaggctctg ccccgaagcg tgggcatccc ccgcgccgga
      841 gcgcgtctgc ccaaaagtct tgcgccgaac caacaagagc tactttaccg acgtggtaga
      901 atagaagcgt tgagataggt attagaacct cccaccagag gcgggagagg gtcagcagga
      961 aggaacactc gtatgagttc aataaaccct gtcgtccagc ttgcgcagca gctcatgctg
     1021 ggaggcgcgg tcaagcaaaa ccacgcgctt gaacacgcca cgattgtgct gctcagcaag
     1081 aaatatcccg atgtccggct ggccggtgtc tcctttgccg ccggattctt tgtcttcggc
     1141 gatgtaccga ccgaggccat tctcccatcg gcacaagaag cgctccattt gctgcgcacc
     1201 acgacgccgg aactcgcgat ccacgaacgc tgcggcacca acctggccgt gacgggtatc
     1261 gtgaccagcc tctcggcgat gactgtcgcc aaaatgcgcc gcccatatgg cagcttcaac
     1321 aacgttatgc tggcgaccac cgccgcgctg atactctcgc gtcccctggg cctcaccgtg
     1381 cagcgctaca taacgacgca gacacccaat gctaccatga ccatcagcgg cgtcaaggcc
     1441 acgagatttc tgggcaatcc cgcccacttc gtcagcaccg ataaccccgg tgccgccggc
     1501 ctgttttcgt agcgaaggtg catcctatgg accgcagtcg ctttcttgta gaaacatcct
     1561 ggcttcacga acgtctcgac gatccccgta tccgcatcgt cgatatgcgt ggctatgtac
     1621 gtactatcga acgtgacggc gtccagctgg cggaatacgt gggcgcgcgt gatgagtatg
     1681 tacaagggca tatccccggc gcggtctata tcgactggtc gcgcgatatc gtagacccgg
     1741 atgacgctgt cgaagcccag gtcgcgtcgc ccgcgcgctt cgctgaggtg atgagcaggc
     1801 tcggcatcgg cgaccagcac cttgtggtgg cttatgatgc gcacccggcc tcgcaatttg
     1861 cgacgcgctt atggtgggcg ctgcgctact acggccatga cgaggtggtg gtcttgaacg
     1921 gcggtctgcc caaatggcag cgcgaaggct cgcccctcac ggctgacgtg ccagcgtatc
     1981 cctctgcaac cttcacgccg cgcgctcaac ccgcattgcg cgcaaccgcc gaggatgtcc
     2041 tgtctctcat aggtaagtcc ggcgtggcgc tgctcgatgc gcgtgatagg ggccagtaca
     2101 gcggcaagct ggtacgcggg catggccgcg cgggccatat ccccggcgcc atcaacatcc
     2161 cccgcgagga ggtcatcgat ccggccagcg gcctcttccg cagtaacgaa gaactgtcac
     2221 ggatcttctc ccaggccaat gtttcaccaa cggagcgcgt cgtcgcttat tgcaacggcg
     2281 gcgtggcggc gactaccatc cttttcagcc tggcaatgct gggctatccg cacctggcaa
     2341 attatgatgg atcatggaac gagtggggca gtcgcgagga tttgccgatt ttgtaggcat
     2401 gctggagga
//
LOCUS       DASZAK010001162         5342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_528127, whole genome shotgun sequence.
ACCESSION   DASZAK010001162 DASZAK010000000
VERSION     DASZAK010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528127"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..3899)
                     /locus_tag="VGT44_20600"
     CDS             complement(72..3899)
                     /locus_tag="VGT44_20600"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2583272.1"
                     /translation="MITLKYVKVEQFRSLRDVELHFPQRGSILITGPNEAGKSTLLES
                     IYFALYGESLAQPRGKRPATLDELVRYGEKRAVVTLALSIGATEITITREIEREKGQS
                     IVVQVRKLGMPAEKPVTDLQKANQRIITELGRMDGKTLRNSCFIEQRGLDRLEQLSGR
                     EREAALHHLLGLEKLARLAEQFSLGIDDERRLAETTLRLRLAEVQARIPELSSKLGDL
                     EAALDAVSVNEDLAAIVQQEADIEEQQQELEHIEARRTELKNSQARISQLNKAGLIID
                     EIIAAYDTIAEAQRELPELEHQIVELERREREELPVLEQRVRELADLTRSFGTLERMA
                     SDLLNAVNTIKELEQGIKQQEHLEETLADLDGQIAYTRQLVEETRQGQHELEEQHRST
                     RPQLEERQKRLQALAEKLTALSAARQTRASRDGQRQQANENFAQIAKLREELSASEQE
                     LALVEKEAQQTQENADAQEKRWRQLSIRHQVQEWHRLKNLSRGLTDAQQHVEAADRQR
                     EQLNSALLATRRSATTQLGIFIACITLAVLSGGGALVEALRQGYAFATIAGMAALVLG
                     AIGGVNLQTYGKTREEERLLDHQLQEANNQVGMMVAAREAALRLGGSQEELAPIEHEI
                     RSLGGSVPGTLEEAQYLLQQAPAHDESIADEQQRLTETRSAAAAARGQVSVTMEAVAA
                     LRKEKARLQELRQQEGWDDFDARLRADQAAIESLQNEITAAAGQEGLPIPNFSDQEPE
                     SQEQPEAELKSKLDETIEATAFQMAAIDDRMDNLPDVATKVKLHQDTLDALLARKLSL
                     VERHEQLESSDPMRQIEQARQQQLALREALRALQDSLRERVVPLGVSFGQTAISIAET
                     TARKQLNALQVALGQKQSLQSRYTDQATRLRDSQEALSEHYRKLAKYSNSLGGWIVPP
                     NPFADALHALRARCDREMQAANEQGILKEQEQLHTREGASRAKIELCRQEIDGTHERI
                     TTLLATRGRSPAKDGTHAEIVAVWPLVGEHSPQDRARLESEIVSTASELRKLEQLDLS
                     LSEKLGIGKTTLDLEQSRRRLAQQERVYATKERAGLLIAATFDRLMRKMLPRTEFYMQ
                     QLLPVLTRGRYHDVSLTTEPEEGISSGGPLQVGLWEPAAKEYIPLASLSGGIADQVSL
                     ALRLAFAIAALPRELNVAPGFLLLDEPLHLANPDRAQSLVEIVVSDQLGQHFEQILFV
                     SHDTPPDASRFPYRVSIDNGQVIESNLPTAPAMETIAVEPEEEGGNGNEHNYTLNGST
                     ENMVLEAVTIANP"
     gene            complement(3934..5217)
                     /locus_tag="VGT44_20605"
     CDS             complement(3934..5217)
                     /locus_tag="VGT44_20605"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV2583273.1"
                     /translation="MQEQDQEQNVITIVLTADNHLGYSIPGQPARKCDELRQRLRRAF
                     QMATDFAIGQGVDLFVQAGDLFDTSHPDEQDRSFVAERLAELKQAGIRAFALGGLHDT
                     PVEMQMHQGDALTAALAPQLSYARLGALHYFPPLAVSQSGETLSPVLLDIRGTLVGLC
                     GFSATQHAGPSGDPLAHIRIDGEIERAAIPLLLLSMPLDEEAVASALSQISFRAVLAG
                     YAHDFQRFTSGQTDVIVAGATQRFDLQDPASEPGFVFLGIAADGVRWCRHVAVDSIHL
                     RRLEIPLAQWWSDTDTTDDEEDAAGATEPTMRLLEELRPLCTEDAMVQLRLTGEISRQ
                     RYHQLELSSIRRYGEAHTFAFDIDDSALTFLPGDETQTSRRSAFEERLSPREELIALA
                     DEWIAAAQDEQERKALLATKEELLAALWTTALHPA"
BASE COUNT          910 a         1637 c         1640 g         1155 t
ORIGIN      
        1 agctggcctg tcaagggcct ccaccaggac aggcacaagg ccccgccgct ctaccccacc
       61 gccccctgtc cctacggatt ggcaatcgtc acggcttcca gcaccatatt ttctgtcgag
      121 ccgttcagtg tataattatg ctcgttccca tttccccctt cttcctccgg ctctaccgct
      181 atggtctcca tggcgggggc ggtggggagg ttgctttcga tgacctggcc gttgtcgatg
      241 gagacgcggt aggggaagcg cgaggcgtcg gggggggtat cgtgcgagac gaagaggatc
      301 tgctcgaagt gctggccaag ctggtcgctg acgacgattt ccacgagact ctgcgcgcgg
      361 tcgggattgg cgaggtgcag aggctcgtcc agcagcagga agccgggggc gacgttgagt
      421 tcgcgcggga gggcggcgat ggcaaaggcc aggcgcaggg cgagcgagac ctggtctgcg
      481 atgccgccgg agagcgaggc gagcgggata tattccttcg cggcaggctc ccacaggcca
      541 acctggagtg ggccgccgct tgagatgccc tcttccggct cggtggtcag gctcacgtcg
      601 tggtagcgac cgcgagtgag tacgggcagc aactgctgca tgtagaattc ggtgcgcggc
      661 agcatcttgc gcatcagacg atcgaaggtt gcggcgatga gcagaccggc gcgctctttg
      721 gtggcataga cgcgctcctg ctgcgccagg cggcggcggg actgttcaag gtcgagagtg
      781 gtcttgccga tgccgagttt ttcgctcagg ctcagatcaa gctgctccag tttgcgcagt
      841 tcagaagcgg tgctgacgat ctcactctcc agacgagcgc gatcttgcgg ggaatgctcg
      901 ccgacgagcg gccagacggc aacaatctcg gcgtgagtgc cgtcctttgc aggcgaacga
      961 ccgcgcgtgg ccagcaaggt cgtgatacgt tcgtgggtac catcgatctc ctgccggcat
     1021 agctcgattt tggccctgga tgcgccctct cttgtgtgca actgttcctg ttctttcaag
     1081 atgccttgct cattggccgc ctgcatctca cggtcgcagc gcgcgcgcag ggcatgtagg
     1141 gcgtcggcaa agggattggg aggcacgatc cagccaccga gcgagttgct gtattttgcc
     1201 agtttgcgat agtgttcgga gagcgcctcc tggctatcgc gcagccgggt agcctgatcc
     1261 gtgtagcggc tctggagcga ttgtttctga cccagcgcaa cttgcagggc attgagctgc
     1321 ttacgcgccg ttgtttctgc gatgctaatg gccgtctgcc caaacgagac acccagcggc
     1381 acaacacgct cgcgcagcga atcttgcaag gcgcgcagag cttcacgcaa ggcaagctgc
     1441 tgctggcgcg cctgctcgat ctggcgcatt ggatcgctgc tttccaattg ctcgtggcgc
     1501 tccaccaggg atagcttgcg tgccagcagc gcatcaaggg tatcctggtg cagtttgacc
     1561 ttcgtggcca catcgggcag gttatccatc ctgtcatcga tggcggccat ctggaatgcg
     1621 gtcgcctcga tcgtctcatc aagtttgctt tttagctcgg cctccggctg ctcctgggac
     1681 tccggttcct ggtcggaaaa attgggaatg ggtagacctt cctgcccggc tgcggcggta
     1741 atctcgttct gaagagattc aatggcggct tgatccgcgc gcaggcgcgc gtcgaaatca
     1801 tcccagcctt cctgctggcg caattcttgc aggcgcgcct tctctttacg cagcgcggcg
     1861 acggcttcca tcgtgacact gacctggccg cgcgcggctg ccgctgcgct gcgggtctcc
     1921 gtcaggcgct gctgctcgtc ggcgatactt tcatcatgcg cgggcgcctg ctgtaaaagg
     1981 tactgcgctt cttcgagtgt gcctggcacg ctgccaccca gcgagcgaat ctcatgctca
     2041 atcggggcca gttcttcctg acttccaccc aggcgcagcg cggcttcgcg cgcggccacc
     2101 atcatgccca cctggttatt cgcttcttga agctgatggt cgagcaggcg ctcttcttcg
     2161 cgcgtcttgc cgtaggtctg caaattgacc ccgccgatcg cgccgagaac cagcgcggcc
     2221 atgcccgcga tagtggcaaa ggcatacccc tgccggaggg cttcaaccag cgcgccgccg
     2281 ccgctcagca ccgccagggt gatacaggcg atgaaaatgc cgagctgtgt tgtggcggaa
     2341 cggcgcgtgg ccagcagcgc ggagttcaat tgctcgcgct ggcgatcagc ggcctccaca
     2401 tgctgctgcg cgtccgtcag cccgcgcgac aggttcttca ggcggtgcca ctcctgcacc
     2461 tggtggcgaa tgctgagctg ccgccagcgt ttctcctgcg cgtcggcgtt ttcctgcgtc
     2521 tgctgcgcct ctttttcgac cagcgccagt tcctgctcgc tcgcgctcag ctcctcgcgc
     2581 agctttgcga tctgcgcgaa gttctcattg gcctgctggc gctgcccgtc gcggctggcg
     2641 cgcgtctgcc gggcagcgct cagcgcggtg agcttctcag ccagtgcctg caagcgtttc
     2701 tggcgctcct ccaattgcgg gcgtgtagag cgatgctgct cttcaagttc atgctgcccc
     2761 tggcgcgtct cttcgactaa ctggcgtgtg taggctatct gcccatccag gtcggccagc
     2821 gtctcttcaa ggtgttcctg ctgcttgatg ccctgctcca gttccttgat agtattgacc
     2881 gcgttcaata aatccgaggc catacgttcg agggtgccga aggagcgcgt gagatcggct
     2941 agctcgcgca cccgttgttc caggacgggt aactcttcac gctcgcgccg ctcaagttcg
     3001 acgatctggt gttcgagttc gggtagttcg cgctgcgcct cggcgatggt atcataggca
     3061 gcgataattt cgtcgataat cagcccggct ttgttcaact gggagatgcg cgcctggcta
     3121 ttcttgagtt cggtacggcg agcttcgata tgctccagtt cctgctgctg ctcctcgatg
     3181 tcggcctctt gctggacgat ggctgccagg tcttcgttga cgcttaccgc gtccagcgcg
     3241 gcctccaggt cgcccagttt gctgctcagt tcggggatgc gcgcctggac ctctgccagt
     3301 cgtaaacgca gggtggtctc agccaggcgg cgttcgtcat cgatacccag ggagaattgt
     3361 tccgcgagcc gcgccagttt ttcgaggccg agcaggtgat gcagcgcggc ctcgcgctca
     3421 cggccactca actgttcaag gcgatccagc ccgcgctgct cgataaaaca ggagttgcgc
     3481 agtgtcttgc catccatgcg ccccaactcg gtaataatgc gttgattggc tttctgtaga
     3541 tcggtaacgg gtttctctgc tggcatcccc aacttgcgca cctgcacaac gatgctttgc
     3601 cccttttcgc gctcgatctc gcgcgtgatg gttatttcgg ttgcgccaat cgagagggcc
     3661 agcgtcacca ccgcgcgctt ctcgccgtag cgcaccagtt cgtcgagtgt ggcgggacgc
     3721 tttccgcgcg gctgcgccag cgactcgcca tagagcgcga agtagatgct ttccaacagc
     3781 gtcgattttc cggcctcgtt cgggccggtg atgagaatac tcccgcgttg tggaaaatgc
     3841 agttcgacat cgcgcagcga gcggaactgt tcaaccttca catatttcag ggttatcatc
     3901 tattcatcca ttccttagaa agccatcctt cgctcaggca ggatgcaaag cggtcgtcca
     3961 gagggctgcc agcagttcct cttttgtagc caataatgcc ttccgttctt gctcatcttg
     4021 agcggcggcg atccattcgt cggccagggc aatcagttcc tcgcgaggag aaagacgttc
     4081 ttcaaacgcg ctacgtcggg aagtttgcgt ctcgtcgccg ggcagaaacg tcagggcgct
     4141 gtcgtcgata tcgaatgcga aggtatgcgc ctctccataa cggcgaatcg aactcagctc
     4201 gagctggtga tagcgctggc gcgatatttc cccggtgaga cgcagctgca ccatcgcatc
     4261 ttcggtgcaa agcgggcgca gttcttcgag caggcgcatc gtcggttcag tcgcccccgc
     4321 cgcgtcctct tcatcatctg tggtatcggt gtccgaccac cattgcgcga gcggtatctc
     4381 caggcggcgc aggtggatgc tatcgaccgc cacatggcga caccagcgca caccatcagc
     4441 ggcaatgccc agaaagacaa aacccggttc gctggcaggg tcttgcagat cgaagcgctg
     4501 cgtcgccccg gcgacgatca cgtccgtctg accagaggtg aagcgctgaa agtcatgcgc
     4561 gtaacccgcc agtacagcgc gaaacgagat ttgcgagaga gccgaagcga ctgcttcctc
     4621 atcaagcggc atggagagca gcagcaacgg gatggccgcg cgctcaatct cgccgtcgat
     4681 gcggatatgc gccagcgggt cgccggatgg ccccgcatgc tgcgtggcgc tgaaaccgca
     4741 gagtccgacc agtgtaccgc gtatatcgag caggacggga gacagtgtct cgccgctctg
     4801 cgagacagcc aatggcggga aatagtggag cgcgccaagg cgcgcatagc tcaattgcgg
     4861 tgcaagcgcg gcggtgagcg catctccctg gtgcatctgc atttcgacgg gggtatcgtg
     4921 cagcccgccc agcgcgaatg cgcgaatgcc ggcctgtttt agctcggcca ggcgctctgc
     4981 cacaaaactg cggtcctgct catcgggatg gctcgtatcg aaaagatcgc cggcttgcac
     5041 aaaaaggtct acaccctggc caatcgcgaa atcggtggcc atctgaaagg cgcggcgcag
     5101 gcgttggcgc agttcatcgc atttacgcgc aggctgacct ggaatgctat accccagatg
     5161 attatcggca gtcagcacaa tcgtgatgac gttctgttcc tgatcttgtt cttgcatcgc
     5221 ggtttcgtac cctccatagc catcctgcca acgaacggcg ttctagcttc gctcaggcag
     5281 gatgcaagga attattctcc atagccatcc tgccaacgaa cggcgttcta gcttcgctca
     5341 gg
//
LOCUS       DASZAK010001163         4624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_242248, whole genome shotgun sequence.
ACCESSION   DASZAK010001163 DASZAK010000000
VERSION     DASZAK010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242248"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2818
                     /locus_tag="VGT44_20610"
     CDS             <1..2818
                     /locus_tag="VGT44_20610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583274.1"
                     /translation="IGGERDKSAPTSWLITSSIGGLMYILEDDQETSDRLRKKDFLNT
                     SYTQAVPNEPVEVGTMGATEHAGWVGGAGEAQPSLPPTQPALPPSNSSSFARLFSPQQ
                     ITSSFKALATPGKHVSGVGSNRAALLAMIVACVAVFFTALDQTVVVTVLPKIIADPGI
                     NISFVHLDHAAWIVSAYLLGFIIAMPLMGRVSDIYGRRRILLLCLLIFGTGSILCGFA
                     PFFGENIDLSFLNFTGIDTSSPGLIFLISARFIQAIGGGAVVPVAMAIVSDFYGQARR
                     GLALGIVGAVTEAGGVVGPLYGALVAQNLDWTYIFYFNVPIVLALMTATWFIIPRGKR
                     LNDGIDWIGALLLGAALTCLSLGLAQQGTDLGPTTINGAAPQNNPISLALAVIFLVLF
                     IIVERKVRHPVIDLSVFKRFTFSASSLVSLLVGAALIIAMADIPLFVDTVMSKSIPEN
                     QVSLFSGLALLRLTAMIPIGAFLGGWLSGRISSRFTGVIGLLFTAVGFYLMSRWPANV
                     DWNQITVSTVTTGLGFGLVIAPISTTAINSVRANQSGMGAAIVTSLRMAGMIFGLAAL
                     TSWALAYFKQLASTFPSLPTTATADQFAKWSQDYANHLINSAHTVYSAVFFTTMFLCL
                     LAIVPAIFLWGNKPAVSEGEIEPEPEALPPSPSDPTEEIQFALAGTFLAGADMYDTVN
                     PSVVPPIPPTLDTMQNGNQGRKPRRRRKKLLLALCALLLLLLIGGVSLAILSNQPDNG
                     SPATTTTGNTPRPGVTTTPTPVSGPRMIELALNDIALTSIFVQQLGSTQGALSNINVA
                     PAANDGVVIALNLTINASGIHRVLPIEIDGVIGLDKQGDIQLQVLHLKRDGLDAGQAA
                     AQSMQQALNQLLITSVMPNLRSTLKAAKLVAVHTSASIVCGAGMEMLTLLIMAPPIQG
                     IPAQPTPTALCFKTPIDFSKLLPS"
     gene            2833..3975
                     /locus_tag="VGT44_20615"
     CDS             2833..3975
                     /locus_tag="VGT44_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583275.1"
                     /translation="MKELRFIRGAWALLLVALVLGVSVITPALASSNTQQHHAATQPR
                     AIAGGTSIPATIYVTLSSIEPQFQARIDQDVPQSFSNAITTLIGKLPQQDQGWALQMA
                     TTLIQPSGSLQSLVTQQNGLAMTVQISLYSGDPKPTVSTMLISFSVLDSSTIQVSASP
                     VSGPALSSGPLETITIPIGILNAVNTTPNCGDAALGFNLQIPVALKGQSTGATTSTAS
                     VMTHGNPMATLQRDSLNNGVNTFVEIPAASLAALSGSLGTIDMGNSLTAQNISIVVQS
                     GAIHILSDVYWSGLNVGTADSTLVPGASNGNLVMHVTSTNFNLFGLFQINENSYDQQI
                     EQLINAKLGNAFAGEFSVSQAQIGPNSHLPCAKSDSLVLTGASSIG"
     gene            complement(4045..4539)
                     /locus_tag="VGT44_20620"
     CDS             complement(4045..4539)
                     /locus_tag="VGT44_20620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583276.1"
                     /translation="MMRQMRRYPRGRYAWRIGALFCALAVLFVVAACSSGTSGQGGSS
                     TATPLTTQNCGSLHSTVAGLLQADKAVAQQDETCFYQAYKQCHPATLTYQAASLDTGA
                     IHHFSITARSGSCTLTDGFQHYIAPNPPGGAITYTCAAVQMQSDGLHITACGNAGDVV
                     IPVQ"
BASE COUNT          904 a         1513 c         1278 g          929 t
ORIGIN      
        1 aatcggcggt gagcgcgata aatcggctcc tacgagttgg ttgattacat cctctatcgg
       61 gggtcttatg tacattttag aagatgacca ggagacatcg gatcgcttgc gcaagaagga
      121 ctttctcaac acgtcctaca cgcaggctgt gccgaatgaa ccggtcgagg tggggacaat
      181 gggcgcgact gagcatgccg gctgggtcgg cggagccggc gaagcgcagc catccctgcc
      241 gccgacgcag ccagcgcttc ctccatccaa ttcatcctcg ttcgcccgct tgttctctcc
      301 acagcaaatt accagttctt ttaaggcgct cgccacgccg ggcaagcacg tcagcggcgt
      361 gggcagcaac cgtgcggcgc tgctggcgat gatcgtggcc tgcgtcgccg tctttttcac
      421 cgcgctcgac cagacggtcg tcgtgacggt gctgcccaaa atcatcgccg atccgggcat
      481 caacatttcg ttcgtccatc tcgatcacgc cgcctggatc gtcagcgcgt acctgctcgg
      541 ctttatcatc gccatgccgc tgatggggcg cgtctccgat atttacgggc ggcggcgcat
      601 cctgctgctg tgcctgctga tctttggcac cggctccatc ttgtgcggct tcgcgccctt
      661 cttcggcgag aatatcgatc tgagcttttt gaatttcacc ggcatcgata cctcatctcc
      721 cggactgatc tttctcatct cggcacgctt catccaggcc atcggcggcg gcgcggtcgt
      781 gccggtggcg atggcgattg tgagcgattt ctatggccag gcgcggcgcg ggttagcgct
      841 gggcatcgtc ggcgcggtaa cagaggcggg cggcgtggtc ggcccgctct acggcgcgct
      901 ggtagcgcag aacctggact ggacctacat cttctacttc aatgtgccaa tcgtgctggc
      961 gctgatgacc gctacctggt tcatcatccc caggggcaag cggctgaacg acggcatcga
     1021 ctggattggc gcgctgctgc tcggcgcggc gctcacctgc ctcagcctgg gacttgcgca
     1081 gcagggaacc gatctcggcc cgacgaccat caatggtgcg gccccgcaaa ataacccgat
     1141 ttccctggcg ctggcggtca tcttcctggt cctctttatc atcgtagagc gcaaggtgcg
     1201 tcaccctgtc atcgacctgt cggtgttcaa acgcttcacc ttcagcgcca gttcgctcgt
     1261 cagcctgctg gtcggcgcgg ccctgattat cgccatggcc gacatcccgc tgttcgtgga
     1321 taccgtcatg agcaagtcca tcccggaaaa ccaggtctcg ttgttcagcg gcctggcgct
     1381 gctgcgcctg acggccatga tccccatcgg cgcgttcctg ggcggctggt tgtcgggccg
     1441 tatctcctcg cgcttcaccg gtgtcatcgg cctgctcttc accgccgtgg gtttctacct
     1501 gatgagccgc tggccggcca acgtggactg gaaccagatc accgtcagca ccgtcacgac
     1561 cggcctgggc ttcggcctgg tgatcgcgcc gatcagcacg acggccatca attccgtgcg
     1621 agcgaatcag tccggcatgg gagcggctat cgtcacatcg ctgcgcatgg ccggtatgat
     1681 cttcggactg gcggccctga cctcgtgggc gctggcctac ttcaagcagc ttgcctcaac
     1741 cttcccttcg ctgccgacga ccgccaccgc cgaccagttc gcgaagtggt cgcaggatta
     1801 tgccaatcac ctgattaact cggcgcacac cgtctatagc gcggtcttct tcacgaccat
     1861 gtttctctgc ctgctggcga ttgtacccgc catcttcctg tggggcaaca agccagccgt
     1921 cagcgaaggc gaaatcgagc cagaaccgga ggcgctcccg ccgtcgccct cggaccccac
     1981 cgaggaaata caatttgcgc tggccggaac cttcctggcg ggcgcggaca tgtacgatac
     2041 cgtgaatccg tccgtcgtgc cgcccatacc gcccacgctc gatacgatgc agaacggcaa
     2101 tcaaggccgc aagccgcgcc gccgtcgtaa gaagctgctg ctggcgctct gcgcgctgct
     2161 cctgctgctg ctcatcggcg gtgtttcgct cgccattctc tcgaaccagc ccgataacgg
     2221 cagcccggct acgaccacaa ccggcaacac gccgaggccc ggcgtcacaa caacgcccac
     2281 gccagtatcc ggcccgcgta tgatcgaatt ggcgctgaac gatatcgcgc taacctccat
     2341 cttcgtgcag cagctcggct cgacgcaggg cgcgctgtcg aacatcaacg tcgccccggc
     2401 agccaacgac ggcgttgtga ttgccctgaa cctcaccatc aacgcgagtg gcattcaccg
     2461 cgtgctgccc atcgaaatcg acggcgtcat tggactggac aaacaggggg acattcagtt
     2521 acaggtgttg cacctcaaac gcgatgggtt ggacgcgggc caggccgccg cgcaaagtat
     2581 gcagcaggcc ctcaatcaat tgctcatcac gtcggtgatg cccaatctac gctccacgct
     2641 gaaagcggcg aagctggtcg cggtgcatac cagcgcctcg atcgtctgcg gcgcgggtat
     2701 ggagatgttg acgctgctca tcatggcgcc ccccatccag ggcattcccg cgcagccaac
     2761 accgaccgcg ctctgcttca aaacgcctat cgatttcagc aagctactgc cgagctaaga
     2821 gaggaaaacg tgatgaaaga actacgcttt attcgcggcg cgtgggcgct gctgctggtt
     2881 gcgctagtgc tgggtgtttc agtcatcaca ccagccctgg cttcgagcaa cactcaacag
     2941 catcatgctg cgacgcagcc gcgcgccatt gccggaggaa cctcgattcc cgcgacgatc
     3001 tacgtgacct tgagttctat cgaaccacag tttcaggcgc gcatcgacca ggatgtgccg
     3061 caatcgttca gcaacgccat cactaccctg atcggcaaat tgccacagca ggatcagggc
     3121 tgggctttgc agatggccac gaccctgatc cagccatccg gctcgttaca aagcctggtg
     3181 acacagcaga acggattggc catgaccgtc cagatctcgc tctactcggg cgacccgaag
     3241 ccaaccgtct cgactatgct catcagcttc agcgtgctgg actcgtcgac cattcaggtc
     3301 agcgcgtctc ctgtgagcgg cccagccctc tccagcggcc cgttggagac gatcacgatc
     3361 cccattggca tattgaacgc cgtgaatacc acgcccaact gcggcgatgc cgccctgggc
     3421 ttcaacctgc aaatacccgt cgcattaaag ggacaatcca ccggcgccac aacttccaca
     3481 gcaagtgtca tgacgcacgg caatcctatg gccacgctcc aacgcgacag cctcaataac
     3541 ggcgtcaata ccttcgtcga gattcccgcc gcgtcgctcg cggcgctgag cggcagcctc
     3601 ggtactatcg acatgggcaa cagtctcact gcccagaaca tcagcatcgt cgtgcaaagc
     3661 ggtgctattc acattctctc ggatgtgtat tggagcggtc tgaacgtggg caccgccgac
     3721 tcaacgctcg tacctggagc cagcaatggc aacctcgtca tgcacgtcac cagcaccaac
     3781 ttcaacctgt tcggactgtt ccagatcaac gaaaacagct atgaccagca aatcgagcaa
     3841 ttgattaacg ccaagctggg caacgccttc gccggtgagt tcagcgtatc gcaagcgcag
     3901 atcggcccca acagccatct cccctgcgcc aaaagcgaca gcctggtgct gacaggagca
     3961 tcaagcatag gataactcca gttcaataaa atagacggac agaccggcgg atgcctgcgg
     4021 catccgccgg tctgtccgtc tattttattg aactggaatc accacatcgc ctgcattacc
     4081 gcacgcggtg atatgcagac catccgactg catctgcacc gctgcgcagg tataggtgat
     4141 cgccccgccg gggggattgg gcgcgatgta gtgctggaag ccatcggtca aggtacacga
     4201 gccacttcta gcggtgatag agaaatggtg tatcgccccg gtatccaggc tggccgcctg
     4261 gtacgtcaac gtagccgggt ggcactgctt gtatgcctgg tagaagcagg tctcgtcctg
     4321 ctgagccaca gctttatcgg cttgcagcag ccccgccact gtactgtgca ggctgccaca
     4381 attctgtgtc gtcaaaggcg tcgccgtgct agaaccaccc tgcccacttg tgccagaact
     4441 gcacgccgcc acaacaaata gcaccgccag cgcacagaat agagcgccta tcctccaggc
     4501 ataacgacct ctcggatagc gtcgcatttg tctcatcatg cttctcctcg tatctagctg
     4561 tgtgaaattg tttctacgag gaagacgaac acagcgcaca aatggttccc caccatccgt
     4621 aaat
//
LOCUS       DASZAK010001164         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_242262, whole genome shotgun sequence.
ACCESSION   DASZAK010001164 DASZAK010000000
VERSION     DASZAK010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242262"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..992
                     /locus_tag="VGT44_20625"
     CDS             270..992
                     /locus_tag="VGT44_20625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583277.1"
                     /translation="MRHSLRSDTKEWDKHTRFWLMIIITLLSSLLIAACDTPGINGNG
                     SNNGVGNGNAGNCNGNNVCNTTNNANSSATPGTPSGVLVSSPTPTPTLEPCPSLLSPS
                     SGVTAIVKGGCQYSITWVFGFKSSNAPGETGINAAGFLNYGSTGNSGGLSNLGIHAGV
                     AYPGGPPTLYAWLVGLGGGCAIVISDSNIQPSAGQLGGAEETSGYVGYPGGPYLPDTY
                     VLAHTTPQPGGGDYQFNLVVAT"
     gene            1358..2854
                     /locus_tag="VGT44_20630"
     CDS             1358..2854
                     /locus_tag="VGT44_20630"
                     /inference="COORDINATES: protein motif:HMM:NF036421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583278.1"
                     /translation="MPHTTSPIARFARHILGRPLYPYQCEIAEAILDSIEGGHGRIFS
                     VMMARQSGKNQLSAVLEAYLLATMPQGTIIKAAPTFTPQIVTSRLRLLSLLDTPLTRD
                     RVWSSDGYMLGLAPRADPALLRNHAGPRIMFYSASPESNVVGATADLLLEIDEAQDVS
                     PDKFDRDFRPMASTTNSTTILYGTAWSDTTLLARQRAINLEHEQRSGLRLHFEHDWRT
                     LAAINPAYRAFVEQEIARLGETHPAIQTQYLLRPITGAGMFLNSLQLTMLQGSHAWED
                     EPRAGGWYIAGMDVAGEARPAAQMSGEARQSSHYGRSKRDSSVLTIGRVHFNEFNLPC
                     IEVTHQQWWTGRSHLDQYAATLALVERWNIRALVIDATGLGAGLASMLASKLGAERVM
                     PFTFTRPAKSRLAYQLLSLLNSGRLKLYEAREAPASIYDECWRQLRLARYRLPASETL
                     DFYVDPVDGHDDFLASLALLTEAVQALPAPPASTLIRPKGLYGSEGRF"
BASE COUNT          636 a          871 c          796 g          591 t
ORIGIN      
        1 atctcgctac aaatcagtgc tgagaaggtt gcgcaggggt accttccaac tcggcgagga
       61 cctcccgcgc ttctcaggaa gcagaagggc gcgaaggcca taaatcaagg ccagaccctg
      121 attaagacgc tgacgcataa aagcctcttt cgacagggaa atgagccaaa tgacactttt
      181 gactgtaaac aggaaatata ctacaatagt agaggttgat tgtctttgct cataagaaaa
      241 gtctcctgta ccaggaaggg agaaaaacag tgcggcattc gcttcgttca gacacaaagg
      301 aatgggataa gcacacccgt ttctggctga tgataataat tactctgctg agtagtctgt
      361 tgatcgccgc atgtgataca ccaggcatca acggcaatgg cagtaacaac ggcgttggta
      421 atggtaatgc tggaaattgt aatgggaata atgtctgtaa cactaccaat aatgcaaatt
      481 caagtgcaac tcccggcaca ccatcaggcg tactcgtttc ttctccaaca cctacaccaa
      541 cgctggagcc gtgtccatca ttgctcagcc ctagttcagg ggtgacagca atagtgaaag
      601 gcggatgcca atactcaatc acctgggtat ttgggtttaa gagcagcaat gctccgggag
      661 aaaccggcat caatgccgcc ggttttctta actacggctc cactggcaac agtggggggt
      721 taagcaacct cggtatccac gccggggtgg catatcccgg agggccgcca accttgtatg
      781 cttggctggt gggattgggt ggaggatgtg ccattgtcat atccgactcc aatatccaac
      841 cctctgctgg gcagctcggt ggtgctgagg aaacatcagg ttacgtaggg tatccgggag
      901 ggccatatct cccggatact tatgtactcg ctcatactac tccccagccg ggaggagggg
      961 actaccagtt caacctggtc gtggcgacat gaggggaaga gggaagaaca cacgctcttc
     1021 atggggcttc tgctgctttg ttcttcctct tccaggtcta agcttccaac ccagaggtat
     1081 gaaggtgagg caaacgggtc gtaatgccat ggaacaaggc cagtcctcgg cttagaggtt
     1141 gacgcatttc ttcgccagtt ttgactcatg acgcataaag gcccgctttg gcgaacttat
     1201 ttgtcaaacg acagccagta tcgccttgca acttcttgca acttctgcat ttccttgaaa
     1261 aacgctcttg aaatcaactc ttcgttatgg tatgctctaa tagcaaccga ccggtgatgt
     1321 ttgcgtaatc atccttcacc tcttttcata atcagcaatg ccacacacca cctcacctat
     1381 cgcgcgtttc gcccgccata tcctcggcag gccgctctat ccttaccagt gcgagatcgc
     1441 ggaagctatt ctggactcta tcgagggagg gcatggccgt atctttagcg taatgatggc
     1501 gcgacaaagc ggcaagaacc agctttccgc cgtgctggag gcgtatctgc tggcgacgat
     1561 gccgcagggg acaatcatca aggccgcgcc gaccttcacg ccgcagattg ttaccagccg
     1621 cctgcgcctg ctctcgctgc tggatacgcc tctaacgcgc gaccgcgtct ggagcagcga
     1681 cggctacatg ctcggcctcg cgccgcgagc cgatcccgcg ctgctgcgca atcatgccgg
     1741 gccgcgcatc atgttctact ccgcttctcc ggagagcaat gttgtgggag cgactgccga
     1801 cctgttgctg gaaatcgacg aggcgcagga tgtctcgccc gacaaatttg atcgcgattt
     1861 cagaccgatg gcttcaacca ccaattcgac cacgatttta tacggcacgg catggtccga
     1921 cacaactctg ctggctcgcc agcgcgccat caaccttgaa cacgaacagc ggagcggcct
     1981 gcgcctgcac tttgaacacg actggcgcac gctggcggcc atcaacccgg cctaccgcgc
     2041 cttcgtcgag caggagatcg cgcgcctggg cgagacgcac ccggccatcc agacgcaata
     2101 cctgctgcgt cccatcaccg gcgcgggcat gttcctgaac agcctgcaac tgaccatgct
     2161 gcaaggcagc catgcctggg aggatgaacc gcgcgcggga gggtggtata tcgccggtat
     2221 ggacgtggca ggcgaagcgc gaccagccgc gcagatgtcc ggcgaagcgc ggcaatcgtc
     2281 tcattatggg cgcagcaaac gcgacagcag cgttctcacc atcgggcgcg tgcatttcaa
     2341 cgaattcaac ctgccgtgca tcgaggtgac gcatcagcag tggtggacgg gccgctcgca
     2401 cctcgatcaa tacgccgcca cgctcgccct ggtcgaacgc tggaacatcc gcgccctggt
     2461 aatcgacgcg accggcctgg gcgctggcct ggcctccatg ctcgccagca aactcggcgc
     2521 ggagcgcgtg atgcccttca ccttcacgcg ccccgccaag tcgcgcctgg cctaccagct
     2581 gctctcgctg ctcaacagcg gtcgcctcaa actctacgag gcgcgagagg ccccggcgtc
     2641 gatctacgac gagtgctggc ggcagcttcg cctggcgcgc taccgtctcc ccgcgtccga
     2701 aacgctcgac ttctacgtcg atcccgtcga cggccatgac gacttcctcg ccagcctggc
     2761 cctgctcacc gaagccgtcc aggcgctgcc cgccccgccc gcctcaacgc tgataaggcc
     2821 aaaggggctg tatgggagcg agggaaggtt ttgaacggat gattgcacac aggatcggcc
     2881 ctgtcatcct gagc
//
LOCUS       DASZAK010001165         2487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_313378, whole genome shotgun sequence.
ACCESSION   DASZAK010001165 DASZAK010000000
VERSION     DASZAK010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313378"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1602)
                     /locus_tag="VGT44_20635"
     CDS             complement(172..1602)
                     /locus_tag="VGT44_20635"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583279.1"
                     /translation="MKLLRSLRVRLLLMFMFVVIVTLATVAFVQAQATANAFQDYTNN
                     AKRIDQVRGRIGQMIDSILIAYRAGDSKSVQIQMMQVATENEVRIILVNSNNRIVLDS
                     NDITLNPVKTASKLSNAPSTPVTSGTQLTPALLETSSLPVILISTNTTLPHLVPTSLP
                     SGTTSTTLAQNFLNAIAHSLWIVIVLAWLLALLLTLALSNRILKPVRELTRVANHMEQ
                     GDLSQRVRIRTQDEMGKLAHAFNTMADSLAHSEQLRRNLVSDVAHELRTPLTNIRGYL
                     EALRDHVIDPTGDMIASLYDESLLLSRLVVDLQELSLAEAGQLRLARRQLSPDEVLIQ
                     AVSALQLQAEAKQLALHVDLPACLPRVEADAERVGQVLRNLLSNAIIHTPPGGTITVS
                     ACIHEDEVLVSVQDTGAGIEAQHLPYIFERFYRADSSRSRTTGGTGLGLAIVKQVVEA
                     HGGHVAAESTPGRGSSFTFSLPVACL"
     gene            complement(1599..2318)
                     /locus_tag="VGT44_20640"
     CDS             complement(1599..2318)
                     /locus_tag="VGT44_20640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583280.1"
                     /translation="MNHSILVVDDDRKTVDLIRLYLEREKYRVLAAYDGQQALMLARQ
                     KYPSLVILDWMLPAVDGLDVCRILRAESNVPIIMLTARATEEDTLQGLDLGADDYVTK
                     PFSPRELVARVRVMLRRAYAQEEPPEELRIGPLLIDFRAHEVWRDGERIDVTPKEFRL
                     LETLAREPGRAFSRLELVQRVFGLDYEGFERTVDVHIMNLRKKIEPSPNEPIYLQTVY
                     GVGYKFRGDLIQLAGEGRKLT"
BASE COUNT          513 a          700 c          741 g          533 t
ORIGIN      
        1 gcaattgagc gagcacctgc taacggtggt cgaggagacg atgcagccga gccacgtctc
       61 gctctggtta cgccctgcca gccacgagag aaccgaacac taccgatcgt gaggtgcgag
      121 cgtacagtcg gccaatcgaa ccttgacggt ccactacagg ggttcagaca cctacaggca
      181 ggccacaggc aaactgaacg taaaagaaga gccacgtccc ggcgtactct cggctgctac
      241 gtggccgccg tgcgcctcga ccacctgctt gacgatggcc agccccaggc cggtgccgcc
      301 ggttgtgcga gagcgagagg agtcggcgcg gtagaagcgc tcgaagatgt aggggagatg
      361 ctgcgcctcg atgccagcac ctgtgtcctg tacactgacc aggacttcat cttcatgtat
      421 acaagcgctt actgtaatcg ttccgccagg gggcgtatgt atgatggcgt tgctcagcag
      481 gttacgcagc acctgcccaa cgcgctccgc gtcggcctcg accctgggca ggcaggcggg
      541 cagatcgaca tgcagggcaa gctgcttcgc ttcggcctgt aattgcaagg cggagacagc
      601 ctggatgagg acttcgtccg gggagagttg cctgcgcgcc aggcgcaact gcccggcctc
      661 ggccaggctg agttcctgta gatcgaccac caggcggctc agcagcagcg attcgtcgta
      721 cagcgaggcg atcatgtcgc cggtcgggtc gattacgtgg tctctaagcg cttccaggta
      781 gccgcgaatg ttcgtcaggg gggtacgcag ctcgtgcgct acgtcgctga ccaggttgcg
      841 ccgcagttgt tcggagtgcg ccaggctatc ggccatcgtg ttgaaggcgt gggccagctt
      901 gcccatttcg tcttgcgtgc ggatgcgcac tcgctggctg aggtcgccct gttccatgtg
      961 gttcgcgacg cgcgtcaatt cacgcactgg cttgaggata cggttgctca aggccagcgt
     1021 caacagcagc gccagcagcc aggccagtac aatcacgatc cagagcgagt gcgcgatggc
     1081 attcaggaaa ttctgtgcca gtgttgtaga agtcgtaccg gatggcagtg aagtgggaac
     1141 caggtgcggc agtgtggtat tggtcgatat tagtattact ggcaaggaag aagtttccag
     1201 cagtgccgga gtcagttgtg ttccacttgt aactggcgta gatggcgcgt tagacagttt
     1261 tgacgcagtt ttgacaggat ttagcgtaat atcattagag tccaaaacga tacgattatt
     1321 ggaattgact aaaataatcc gtacctcatt ttctgttgct acttgcatca tctggatctg
     1381 tacactcttg gaatctcccg cccgataagc gatgagaata ctatcgatca tctggcctat
     1441 tcttccacgc acctggtcga ttcttttggc gttgttggta tagtcctgga aggcgttggc
     1501 tgtcgcctgc gcctggacga aggcgacggt tgccagcgtc acgatgacga cgaacatgaa
     1561 catcagcagc agacgaacgc gcaggctacg cagcagtttc atgtcaattt ccttccttcc
     1621 ccggcaagct ggataagatc gcccctgaac ttataaccaa cgccgtagac cgtttgcagg
     1681 tagatcggtt cgttggggga tggctcgatc ttcttgcgca ggttcatgat atgcacatcg
     1741 acggtgcgct caaagccttc gtagtccagg ccaaagacgc gctgcaccag ttccaggcgg
     1801 ctaaacgcgc ggcccggctc cctggcgagc gtctccagca ggcgaaattc tttgggggtc
     1861 acgtcgatac gctcgccatc gcgccagacc tcgtgcgcgc gaaagtcgat cagcagcggc
     1921 ccgatgcgca gttcttcggg cggctcctct tgcgcgtaag cgcgccgcag catgacgcgc
     1981 acgcgcgcca ccagttcgcg cgggctgaac ggcttggtaa cgtaatcatc cgcgcccagg
     2041 tcaagcccct ggagtgtgtc ctcctcggtc gccctggcgg tcagcatgat gatcggcacg
     2101 ttcgattcgg cgcgcaggat gcggcagaca tccagcccat cgactgctgg cagcatccaa
     2161 tcgaggatca ccaggctggg gtatttctgg cgagccaaca tcagagcctg ttgcccatca
     2221 tacgccgcca gcacccggta cttttcgcgt tccaggtaca ggcgaatcag gtcaaccgtt
     2281 tttctatcgt cgtccacgac gaggatactg tggttcatat gcagaatgca aggaagtata
     2341 tgtccccgcg taggagtacg gtaaaccggc tcctacgcgg ggagtgatag cctacaaaca
     2401 attcgtatgt cctaagtata cacaaaagtt gtgaagttct tatgaagcag aggggtttac
     2461 cgcatcctga tcgattcttc atcagtt
//
LOCUS       DASZAK010001166         6172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_457578, whole genome shotgun sequence.
ACCESSION   DASZAK010001166 DASZAK010000000
VERSION     DASZAK010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6172
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457578"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..1398
                     /locus_tag="VGT44_20645"
     CDS             85..1398
                     /locus_tag="VGT44_20645"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2583281.1"
                     /translation="MSCRPRNCPIFARKACGFTERHLMEVVNLNLAHRWYVGYDLGEA
                     LPHHSSLSKIRTRYGLAVFERFFEQIVERCCEAGLVWGEELYFDGTRVQANASMQKMK
                     PRFYVQAKDHVVSLFAQEEQTPSEEEHDSNEQQVGASSLVQRYGTYQETERSASRYHP
                     LADYRINQTDPDATPLRSSGEDKPHCGYHLHYVVDGGKARVILAALVTPATITDNIPM
                     LDLARWVRFRWKVHPRIAVGDTKYGTIANIAELEQDGIRAYLPMRDYANDMPFFPPEQ
                     FRYDARRDIYWCPQDQQLTRWTNKTEAQAVLYRADAAVCNACPVKAQCTDSTSGKALR
                     RSWFQEELDRVKGYWDTAAYAKAMRKRQVWVEPMFAEAKEWHHLWRFRLRRLEKVNIE
                     ALMTAAGQNLKRLLNQRKRRPRPKGEASAMAISKLPASSEERLAS"
     gene            1570..2490
                     /locus_tag="VGT44_20650"
     CDS             1570..2490
                     /locus_tag="VGT44_20650"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV2583282.1"
                     /translation="MKRTAHPALSPEGLEALASYESWLREREDLAAASIRNYLSDLQH
                     FIAWYETEREGHVHDSFTPQGITTPALTRYRSYLQTVQRQKPASVNRTLIRLKRYFGW
                     VIQQHLITYDPSTAVKLVGQEESAPRHLDDQEEQALVAAVTKAGTLRDRVLIVLLLHT
                     GLRAREICRIRRDQVKLGKRSGLLEIIGKRNKYREVPLNATSRKVLEEYLPTLPSDAI
                     FLFPSGKTRKALSERALGSIVKKYACSAKLPDVSPHDLRHRFGYRMAESVPLHRLAQI
                     MGHDSLDITRLYIQGTKHDLQQAVETIAWT"
     gene            2578..3120
                     /locus_tag="VGT44_20655"
     CDS             2578..3120
                     /locus_tag="VGT44_20655"
                     /inference="COORDINATES: protein motif:HMM:NF024954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2583283.1"
                     /translation="MRPYLRVRSLSQEEYTQLKRMSISRKLCAGRVKRAQIILLSNQG
                     HLPTEIDKKLNIHERTARRWIGRFNRLGIAGLEEGPREGRPRVYSTEEVGVVLQTALT
                     PPSQLDQPFHSWTLDRLVIYLTEVKGIAIKRSRLSEIFRHEGLRWRHQEGWFGERIDP
                     EFAQKRGPSKPSTPPLQTTV"
     gene            3147..3713
                     /locus_tag="VGT44_20660"
     CDS             3147..3713
                     /locus_tag="VGT44_20660"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2583284.1"
                     /translation="MSARSHPGPELVATQPTISKPAGRAKQEVDYGRTQREGYVFGAL
                     QPASGEVVTATYTSRSIKNFVDFLDQVEATIPTSTERIYAVLDNLATHKAYDVLLFNL
                     AHPRWEFVFQPKYAAYLNLIEPWWKTLKSLALKGKRFETWEEVEPAIARASSYWNKHK
                     HPFVWGKRRRHRPPRKLGIACLPKATSI"
     gene            complement(3830..4195)
                     /locus_tag="VGT44_20665"
     CDS             complement(3830..4195)
                     /locus_tag="VGT44_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583285.1"
                     /translation="MVDGYGLLQRHAQGVRIAVLLGGLGPCQRLLKLAHIEAERRIRA
                     PLQRLGVCVHKMASFRECLLQAMHQLAQVGARLGFGRVRPEQKGEVVASLGCIAMEHE
                     IGQQRSQARRVNVADKRCA"
     gene            4091..>6172
                     /locus_tag="VGT44_20670"
     CDS             4091..>6172
                     /locus_tag="VGT44_20670"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEV2583286.1"
                     /translation="MSEFEEALTGAEAAEQHSDPHALRVALEQAIAVYHGDLLPSCYD
                     DWILPERERLRQAFTGALERLLLLLESQGDPRAAIACAQRLLRHDPLREDTYRTLMRL
                     QAACGDRAGALRVYHTCATVLERELGVEPSAATRAAYAHLVKGEAQARPVMPSPPANT
                     NLPVPLTSFVGREAQMAEITHLLQTTRLLTLTGPGGCGKTRLALEGATHVVSEYADGV
                     WLVELAPLADPALVPQTVAATLGVREQPGHPILGALMDYVRTKTLLLILDNCEHLIET
                     CAHTAEGLLRVAPGVTILASSRESFGIAGETSYRVPSLSLPDPRQLHDLDALAQNACV
                     RLFVDRASAAYPPFRLTAKNAPAVAQIGLRLDGIPLAIELAAARTKVLPPEQIAARLS
                     DRFRLLTGGSRTALERHQTLQALIDWSHQLLSEAERVLLRRLSVFAGGWSLEAAQAVC
                     GDELVDRVLETLARLVDKSMVVVEEPMAAGEGRYRLLETIRQYARDKLLASGESEAVR
                     ECHLEFFLRFSEEVEPKLRGAEQVAWLDRVETGYDNLRTALAWSLERGKCESALRLAG
                     ALYYFWELRGYWSEGQKWLDEALAQASRAPSGRGAVGDPSLPVRTQAARRAKALYAAG
                     RLHFLTLDLEGARTMLEESSRLWRELGDKWWMAAALELLGLLSLFEGDLQTARARWEE
                     GVSLGRAVEDRW"
BASE COUNT         1274 a         1768 c         1870 g         1260 t
ORIGIN      
        1 ataactgcgc gagtgacgtt tggctcagtg tttctcgaag gccagcgaac aaccgttctc
       61 tgaagtcgct gatgcttgtt ttccatgtct tgccgcccgc gtaattgtcc gattttcgcc
      121 cgcaaagctt gcggatttac agagcggcac ctcatggagg tagtcaacct gaacctggca
      181 catcgttggt atgtggggta tgatctcggt gaggcccttc cccaccatag ctccttgagc
      241 aaaatccgca cccgctatgg cttagcggtc tttgagcgct tctttgaaca gatcgtcgaa
      301 cgctgttgtg aggcaggatt agtctggggc gaggagttgt actttgatgg gacgcgtgtc
      361 caagccaatg cctccatgca aaagatgaaa ccacgatttt atgtgcaagc gaaggaccat
      421 gtggtgagct tgttcgcaca ggaggagcaa actccttctg aggaggagca cgactcaaac
      481 gagcagcagg tcggagctag cagtctggtg caacgctatg gaacgtatca ggaaacggag
      541 cgcagtgctt cgcgctatca tccgctggct gactatcgca tcaaccaaac tgatcctgac
      601 gccaccccct tgcgttcctc tggcgaggac aagccacatt gtggctatca cttgcattat
      661 gtcgtcgatg gaggaaaagc gcgggtgatt ctggcggcct tggtgacgcc ggcaaccatc
      721 accgacaaca taccgatgct cgatttagcg cggtgggtgc gttttcggtg gaaggtgcat
      781 ccgcgcattg cagtgggtga taccaaatat ggaaccattg ccaacattgc cgaactcgaa
      841 caggacggga ttcgagctta tcttcctatg cgggactatg ccaatgatat gccgtttttc
      901 cctccggaac agtttcgtta tgatgccagg cgtgacatct actggtgtcc gcaggatcag
      961 cagttaactc gctggacgaa caaaaccgaa gcacaggctg tactctatcg ggctgatgcg
     1021 gcggtctgta atgcgtgccc agtcaaggct cagtgtacgg acagtacaag cggcaaggcc
     1081 cttcgccgtt cgtggtttca agaagaactt gaccgggtga agggctattg ggacaccgca
     1141 gcctatgcca aggccatgcg caagcgtcag gtgtgggtcg agccgatgtt tgccgaggcc
     1201 aaggaatggc atcatttgtg gcggtttcgt cttcgccgac ttgaaaaggt gaacattgag
     1261 gccctgatga ctgcagctgg acagaatctc aagcgcctcc tcaatcaacg caagcgcaga
     1321 cctcggccca aaggagaggc gagcgcaatg gccatctcga agcttcccgc ttcttctgaa
     1381 gagcgtttgg cttcttgatg aggaagagca ctttttgtag agctacgtct gtgatttttc
     1441 aacacgctga tacctttgca gaaataaatg tggcaatgca ccaggacttt tttctgctaa
     1501 tgttgccact atcccttgaa agacatttct gctaaggttg ttggcacgag cgagaagaca
     1561 aggtggtcca tgaagcggac ggcacatcca gcactttcac ccgaaggcct tgaggcactg
     1621 gcttcctatg agagctggtt gcgcgagcgc gaggatttgg cggcggcatc cattcgcaat
     1681 tacctgagcg acttacaaca cttcatcgcc tggtatgaaa ccgagcgaga ggggcatgtc
     1741 catgattcct ttaccccgca aggaatcacc actcctgcat tgacgcggta tcgaagctat
     1801 ttgcagaccg tgcaacgaca gaaaccggcc tcggtcaacc gtaccctcat taggctgaag
     1861 cgctacttcg ggtgggttat acaacagcac ctcatcacct atgatccttc caccgctgtc
     1921 aaactcgtgg gacaagagga gagcgcccct cgccatctgg acgatcaaga agaacaggcc
     1981 ctggtggcgg cggtcacgaa agcaggcacc ctcagggatc gcgtcctcat cgtcctcttg
     2041 ctgcacacgg gactgcgcgc gagggaaatc tgccgaatcc gacgtgacca ggtaaaacta
     2101 ggtaagcgga gcggcttgct cgaaattatt gggaaacgca acaagtatcg cgaggtgccg
     2161 ctgaacgcga cctcccggaa agtgttggag gaatatcttc ccactcttcc atctgatgct
     2221 atcttcctct ttccatcagg caagacgaga aaggctttgt cagaacgcgc tctgggctct
     2281 attgtcaaaa aatatgcatg ttccgcaaag ctgccagatg tcagtcctca cgatttgcgg
     2341 caccgctttg gctatcgcat ggcggagtct gttccactcc accgtctggc acaaatcatg
     2401 gggcatgact cactggacat caccaggctc tacatccagg gaacgaagca cgatttgcag
     2461 caagcggtcg aaacgatcgc gtggacatag aagaggctta gtggtgcatc tgctaagttg
     2521 tgtgccataa tgagaggagt aaatgaagcg gttcacgttc agcagaggag gtgcctgatg
     2581 cggccttatc ttcgtgttcg ctctctttcc caggaggagt acacccaact caaacgcatg
     2641 tccatctcgc ggaaattgtg tgctggtcgc gtcaagcgtg ctcaaatcat tcttctctcc
     2701 aatcagggcc atcttcccac tgagatagac aagaagctga atattcacga gcgaacggct
     2761 cgccgctgga tcggcaggtt taatcgctta ggcatcgcgg gcctggaaga aggccccaga
     2821 gaaggccgcc cacgggtcta ttccacagaa gaggtggggg tcgtccttca aactgcctta
     2881 accccaccta gccaactgga tcagcccttt cactcctgga cgctcgaccg cttggtgatc
     2941 tatctcaccg aagtgaaagg cattgccatc aaacggagcc gattgagcga gattttccgg
     3001 catgaaggtc tgcgatggcg gcatcaagag ggctggttcg gtgagcggat tgatcccgag
     3061 ttcgcccaaa aaagggggcc atcgaaaccc tctacacctc ctctccagac aacagtgtag
     3121 tcgtctgcat tgatgagatg ggaccattgt cagcacgctc ccatcctggg ccagagttgg
     3181 tagccaccca accgacgatc agcaagcccg ctggcagggc gaaacaggag gtggattatg
     3241 gaagaacgca gcgagagggc tatgtcttcg gagcgctcca accagctagc ggtgaggtgg
     3301 taacggctac gtacaccagc cggtctatca agaatttcgt tgacttcttg gaccaggtcg
     3361 aagctacgat ccctacttcg acggagcgaa tctatgctgt gctggataat ttggcgacgc
     3421 ataaagccta cgatgttctg ctttttaact tggctcatcc acggtgggaa tttgttttcc
     3481 agcccaaata tgcggcctat ctcaatttga ttgagccgtg gtggaagacg ctgaagtcgc
     3541 ttgcactcaa aggcaagcgg ttcgagacgt gggaagaggt cgagcctgcg attgcgagag
     3601 ccagttccta ctggaacaag cacaagcatc cctttgtctg gggaaaacgg cgcagacatc
     3661 gcccaccaag aaaacttggc attgcctgct tgcccaaggc gactagtatt tagcggatgc
     3721 accattagcg ttagaaatgt tgtcggcggg ctgaagtcgg cctgcgtgaa aggaagccag
     3781 agcgatgccc gagttgcaca ttcacctgct cggcgatttc cgcctcgcct caggcgcatc
     3841 gcttatcagc aacgttaacg cgccgcgcct gcgatcgctg ctggcctatc tcgtgctcca
     3901 tcgcgatgca ccccaagctc gccaccacct cgcctttctg ctctggcctg acacgaccga
     3961 agcccaggcg cgcaccaacc tgcgccaact ggtgcatggc ttgaagcaga cactccctga
     4021 agctggccat tttgtgcaca cagacgccca aacgctgcag tggcgccctg atgcgccgtt
     4081 ccgcctcgat gtgagcgagt ttgaggaggc gctgacaggg gccgaggccg ccgagcagca
     4141 cagcgatccg cacgccctgc gcgtggcgct ggagcaggcc atagccgtct accacgggga
     4201 cctgctgccc agttgctacg acgactggat cctgcccgaa cgtgagcggt tgcgccaagc
     4261 ctttacggga gcgctggaac gcttgctgct gctcttggaa agccagggcg atccgcgtgc
     4321 tgcgattgcc tgcgcccaac gcctgttgcg ccacgacccg ctgcgcgagg acacctaccg
     4381 caccctgatg cgcctgcagg cggcctgtgg tgaccgcgcg ggggcactgc gcgtctatca
     4441 cacctgcgct accgtcctcg agcgcgaact gggggtcgag ccgagcgcgg ctacgcgggc
     4501 cgcctatgcg cacttggtga agggagaggc gcaggcgcgg cccgtgatgc cctcgccgcc
     4561 agcgaacacc aacctgcccg tgccactgac cagtttcgtg gggcgcgagg cgcagatggc
     4621 cgagatcacc cacctgttgc aaaccacccg cctgctcacg ctcacggggc caggcggctg
     4681 cggcaagacg cgcctcgcgc ttgagggggc gactcacgta gtgagtgagt atgcggatgg
     4741 ggtgtggctc gtcgaattgg cgcccctcgc cgacccggcg ctcgtcccgc aaacggtggc
     4801 ggcgacgcta ggcgtccggg agcagcctgg acaccccatc ctgggcgcgc tcatggatta
     4861 tgtgcggacg aaaaccctcc tgctgatcct ggacaactgt gaacacctga tcgagacctg
     4921 cgcccatacg gccgagggcc tgctgcgcgt ggctccgggc gtgaccatcc tggccagcag
     4981 ccgtgaatca ttcggcatcg ctggcgaaac atcgtaccgc gtgccgtccc tctcactgcc
     5041 ggacccacgg caactccacg acctcgacgc cctcgcgcag aacgcctgcg tgcgactctt
     5101 tgtggatcgc gcctcggccg cgtacccacc ctttcgcttg accgcaaaga acgcacccgc
     5161 cgtcgcgcag atcggcctgc gcctggacgg gattccgctg gcgattgaac tcgcggcggc
     5221 gcgcaccaag gtccttccgc cggaacagat cgcggcgcgg ctctccgatc gctttcgcct
     5281 gttgacgggg gggagtcgca ccgcactgga gcgccatcag acactgcagg cgctgataga
     5341 ctggagccat cagttattgt ccgaggcgga gcgcgtgctg ctgcgccggc tctccgtctt
     5401 tgccggcggg tggtcgcttg aggcagcgca ggcggtgtgc ggtgacgaac tggttgacag
     5461 ggtactggag acgctcgcgc gcctggtgga taaatcgatg gtcgttgtcg aagagcccat
     5521 ggcggcgggt gagggacgct atcgcttgct cgagaccatc cggcagtacg cgcgcgacaa
     5581 gttgctcgca tcgggcgaat cggaagcggt ccgcgagtgc cacctggagt ttttcctgcg
     5641 cttctcagag gaggtggaac cgaagttgcg cggcgcagag caggtggcgt ggttggaccg
     5701 cgtggagacc ggctacgata acctgcggac cgcgctggcg tggtcgctgg aacgtggtaa
     5761 gtgtgagagc gcgctgcgac tggcgggagc gctctattac ttttgggaat tgcgcggata
     5821 ctggagcgaa gggcagaagt ggcttgacga ggccctggcg caggcttcgc gcgcgccgag
     5881 tggaaggggt gcggtgggag acccctctct tcctgtccgc acgcaggcag cgcggcgtgc
     5941 caaggcgctc tatgctgctg ggaggctcca ctttttgaca cttgacctgg agggtgcgcg
     6001 cacgatgctc gaagagagct cgcgcttgtg gcgcgagctg ggagacaagt ggtggatggc
     6061 tgcggctttg gaattactgg gactgctctc gctatttgag ggcgacctcc agacggcgcg
     6121 tgctcggtgg gaggagggcg tgtcgctggg gcgagcggtc gaggaccgct gg
//
LOCUS       DASZAK010001167         7336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_670012, whole genome shotgun sequence.
ACCESSION   DASZAK010001167 DASZAK010000000
VERSION     DASZAK010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7336
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670012"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..686)
                     /locus_tag="VGT44_20675"
     CDS             complement(81..686)
                     /locus_tag="VGT44_20675"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HEV2583287.1"
                     /translation="MSTNLGSVDILGAEAVGQAGQRRFRLFALAGARSLVLWMEKEQL
                     NTLSLALDRVIAQITDGALLRTIAQSTPLPQPEGLPANFPRRPSIEFQVGQIRIGYDE
                     QNNLLALIASPIEVLLEPGQEPRAVIRDDESVSFLFTVQQSQQLTKTISALVVSGRPV
                     CPLCNTPLDGSPHACIRQNGHREVAQELEEDEDIDDIDEEE"
     gene            complement(733..1719)
                     /locus_tag="VGT44_20680"
     CDS             complement(733..1719)
                     /locus_tag="VGT44_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEV2583288.1"
                     /translation="MVSTRLKVASAFRFSSESQQMLNEAAGTQIIYVTSDEELAACLA
                     NLDVLCGYTLPANWREAAPRLHWLQFPGAGVDSLQSSGLLERGSPIVITTAAGIHATT
                     IAEYVFGSMLMFNWNWPQMLRLQDQHIWARSASWYNLGKHELSGQTLGIVGLGSIGRR
                     IAQVGRAFRMRVLATRHTPPREGQHDPDADELFPRERLHDLLSQSDYVVLSIPLSPDT
                     EGLIGEAELRAMRQDAYLVNVARGRIVDEAALIRALREGWIAGAGLDVTETEPLPAES
                     PLYTMPNVILTPHISGNSIHYNERLAALFADNLRRYRAGEPLLNRYDPARGY"
     gene            2083..3777
                     /locus_tag="VGT44_20685"
     CDS             2083..3777
                     /locus_tag="VGT44_20685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583289.1"
                     /translation="MLVNNDVSRGGGSNSGLESKNGQSALGWKELKPASRKTKGRWRR
                     RLFTFVTAVLLLTLIFAAVRAASVASSTDEQLTLSIGGQQSALLDLRQSVPISPYLNG
                     VNVFPEQGSNSIDAISSGFMSYGPEVVNGLKSAGVSLLRFPGGSWGEQQTLTNHILSY
                     QQLYDFSNLLSETGAQGMIQARISSPSDKFSKYTSLDLRADLAGSWVDFMNNPKSQWR
                     AKNGYAKKQIHPVLLWSVGNEPDNLLNPDTGKPFTVADYVKDFIQFSIVMHQNDPQIT
                     VLGPELSQFDGIGVGPADSQGQLWMEGFLQGVASYEKAHNLPSKTMPFHLLDGVSFHA
                     YPFLDGKTSPYVLMSSPQEWYYLLPQLRQTIRQIMGRDLPVAITEINATPSSAQHPSI
                     GNEALWWADTLGTLMNEQADYVSYFSAQGVPQPYPLFTGDQAPQQTATYRVFQLFSHL
                     QRNLIPLQVQHDPVDAFATVDNTRQTISIMFVNKSAGNQTAQISSLNQFFGFSPWQSQ
                     EVSIAGYSIVVITLHRNGGAQAYSYVVPTADTTAITPLKQTVCGSKYDPLAFATPC"
     gene            3793..5073
                     /locus_tag="VGT44_20690"
     CDS             3793..5073
                     /locus_tag="VGT44_20690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2583290.1"
                     /translation="MALTYIGYFFLSIYLLIILLMVFQAVFNIRLRLFIWETPEHAHL
                     NHAPTIYRDPCLSFTILLPAKNEEDVYRETLQKVYDLNYPRELMQIVAICREDDPGTI
                     AEAQAKIDELGDPNVQLLIYNDYPISKPHGLNLGLQVARGDIVTIFDAEDEPHPDILN
                     IINTTMLNEDVDAVQSGVQLMNHNTKWFCFLNVLEYFFWFKSSMHFFARIGMTPLGGN
                     TVFVRRELMEQLGGWDETCLTEDADLGIRLSLSHARIRIIYEDEFVTREETPHTIEQF
                     IKQRTRWNQGFIQILFKWEWLKLETLSQRLLAFYVLILPEVQALFAILVPVAIIMFFT
                     VKFPLWLAMLTFAPLYCFVLDVFMDVAGLHEFLKAHGQKWSWREAFILVMAFFPYQWI
                     LSLGALRAVYRATRGATNWEKTAHIGQHREKIAA"
     gene            5096..>7336
                     /locus_tag="VGT44_20695"
     CDS             5096..>7336
                     /locus_tag="VGT44_20695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2583291.1"
                     /translation="MTVSYTPQQDQDPQQPARQTPGADAPGTARQPGSPTDEPTRVVE
                     PIRRKIVVRSPVTPSSGMRYFASDQADGPQSQPLTGQPLTPNEPRPQNGVADVDRLPT
                     SALPAPSAPPVDGHYRQLGQQVQNIRQDMAQVRQPSALRNGASNSVIDRIATAVSPAS
                     TPSAPPSRAATASSRGTAARPEVENGNIILYRTHNFFLRTAFRSGQTGNPLRRPSGHT
                     SVMPRVMPDQEKRVAASDTHVMPSIIVNPGKQARAIPIPSWSEAILVMFALIGTCVAH
                     AYNMFNFPRYELDEGTYMSYAWAILHGNIASEPYGYGHPPLGWIQIAGWIQMTGGFFT
                     FGTAINSGRVLMLLYALGSALLVYLIVRRMSGSASAGLLALVIFSFSPLSITYQRQVF
                     LDNIAVFWLLMSFYLLVVGNSRLKFIIGAAISFGVAFLSKETSAVFFPAIVYAAWLYS
                     TKFQRKFSMTAFIYTIMAVCSTWVLMAVLKGELFPYTWHLPWDTHPHLSMLDTFVGQA
                     QRGVESGSLFSSFQIWQENDPVFIILSIAAPAFNLLYGWWNRKHLMLALMAALYWLLL
                     VRGGQVLSFYVIMLIPVTALNTAMALNAILNWGSRIGHWRWLNMAFTVTRALLLLVAL
                     AAILPYDIQETSFRFYQHPTSAQEQAMVWIRDHVSRDSFIVINSYLYLDLRLPGGTGV
                     GDSAPFPHAEVYWEVAYDPTLHDKGGALQDNWDRIDYIVADSEMLNDIKTYGGPMDLI
                     KTAYEHS"
BASE COUNT         1562 a         2217 c         1971 g         1586 t
ORIGIN      
        1 tggtgcttca caaacttatg attgaggcag acgaggaact ttacctgcaa aaagccaaca
       61 aatacctttg cagcgaataa ttattcttcc tcgtcgatat cgtcgatatc ctcgtcttcc
      121 tccagttcct gagcgacctc gcggtgtcca ttttgccgga tgcaggcatg ggggctgcca
      181 tctagcggag tattgcagag cgggcagacg gggcggccac ttacgacgag cgcgctgatg
      241 gtttttgtca attgctggga ttgttgtacg gtgaagagga aagagaccga ttcatcgtcg
      301 cgaatgacag cgcgtggctc ctgtccaggc tcaagcagca cttcgatggg cgaggcaatc
      361 agggccagca aattattttg ctcgtcgtag ccgatgcgaa tctgccctac ctgaaattcg
      421 atcgaaggac ggcgaggaaa gttggcgggc aagccttctg gttgtggcag tggcgtggac
      481 tgggcgatgg tgcgcagcaa cgcgccatcg gtaatttgcg cgatgacccg gtctagcgca
      541 agcgagagcg tattcaactg ctccttttcc atccagagta cgagcgagcg agcgcccgct
      601 agcgcgaaca ggcgaaagcg gcgctgcccg gcctggccaa cggcctcagc gccgaggatg
      661 tccacagaac ccaggttggt actcatctca aaatcttcct cacaacagta ggaacagctt
      721 gatacgccag ggttaatacc ccctcgctgg atcatagcgg ttcaacaacg gttcaccggc
      781 gcgatagcgg cgcaggttat cggcgaacag agccgccagg cgctcattat aatgaatact
      841 gttccctgaa atatgcggtg tcagaatcac attaggcatc gtgtacaatg ggctttcggc
      901 gggcagcggc tccgtctccg tcacatccag tcccgcgccg gcgatccatc cttcacgcaa
      961 ggcgcgaatc agcgcggctt catcgacaat gcgcccgcgc gcgacattca ccaggtaagc
     1021 gtcctggcgc attgcccgca attcggcttc accgatcagt ccctccgtat caggcgataa
     1081 ggggatggat aaaacaacat agtcactctg gctcaagagg tcatgcaggc gctcgcgcgg
     1141 gaaaagctca tcggcatcgg ggtcgtgctg cccctctcgt ggcggtgtat ggcgcgtcgc
     1201 cagcacacgc atcctgaagg cgcgccccac ctgggcaatg cgtcgaccga tactgcccag
     1261 cccaacgatc cccagcgttt gcccagataa ctcgtgcttg cccaggttat accagctcgc
     1321 gctcctggcc cagatatgtt gatcttgcag gcgcagcatc tgcggccagt tccaattgaa
     1381 catcagcata ctgccaaaaa catactcggc aattgtcgtg gcatggatac ctgccgccgt
     1441 cgttatcact atcgggctgc cgcgttcaag cagcccgctc gactgtaacg aatccacccc
     1501 cgcgccgggg aattgcagcc aatgcagacg cggagcggcc tcgcgccagt tcgcgggcag
     1561 cgtatagccg cacagaacat cgagattggc aaggcaggcc gccaactcct cgtcgcttgt
     1621 cacataaata atctgcgtcc ccgccgcctc gttgagcatc tgctgcgatt cggatgagaa
     1681 acgaaacgcg cttgctactt tcaaacgagt agaaaccatt cagcactcct cgataatcaa
     1741 ctccgtaggg gaataacgcc acaatacgta gtctatacga tgcaacggct ctgtggcaag
     1801 tctactcttg catgatacta tcgtttgcat gatttgatac agatgtttct tgtgcatctg
     1861 tggcaagttt gttacgctac acatcaagtt ttctcggcgc gaagccgata attacggaca
     1921 ggagtatatc ctggaggtag gttggacaat tacctatgtc ctacttttct cgtcattcga
     1981 tgcagtcgga tgtgatagcg cgtttgaaca gttagataca tcaatgaaat aagggatatc
     2041 cgggcgcgca agcagccatc tctattaggg agtttatcat atatgctggt aaataatgat
     2101 gtgagccggg ggggaggctc taattcaggg cttgaaagca agaatgggca atccgccctg
     2161 ggatggaagg agctcaagcc ggcctcgcgc aaaacgaaag gccgctggcg tcgccgcctc
     2221 tttacattcg tgaccgccgt gctgctgttg acgcttattt tcgctgctgt gcgggcagcc
     2281 agcgtggcca gcagcaccga cgagcaactc acgctgagca tcggcggtca acaaagcgcg
     2341 ctgctcgacc tgcggcaaag cgttcccatc agtccctatc ttaacggcgt caatgtcttc
     2401 ccggagcaag gctccaactc gatcgacgcc atctcttccg gctttatgtc ctatggcccg
     2461 gaggtggtga atgggctgaa aagcgcgggc gtcagcctct tgcgtttccc tggcggcagt
     2521 tggggcgagc agcagacgct caccaaccat atcctatcgt accagcaact ctacgatttc
     2581 agtaacctgc tctctgagac cggggcgcag ggtatgatcc aggcgcgtat ttccagccca
     2641 tctgataaat ttagtaagta taccagtctt gatctgcgcg ccgacctggc gggcagctgg
     2701 gttgatttca tgaacaaccc caaaagccag tggcgcgcga agaacggcta tgcaaagaaa
     2761 cagattcatc ccgttctcct ctggtcggtg ggcaacgaac ccgataatct gctcaacccg
     2821 gacaccggca agcccttcac ggtggcggat tatgtcaaag actttatcca gttctcaatc
     2881 gttatgcacc agaatgaccc ccagattacc gtcttggggc cggaactcag ccagttcgac
     2941 ggcatcggcg tagggccggc ggattcccag gggcaactct ggatggaagg tttcctgcag
     3001 ggcgttgctt cgtatgagaa agcgcataac ctgccctcta aaaccatgcc ctttcatctg
     3061 ctcgacggtg tctcgtttca tgcctacccg ttcctggatg gcaagacttc accctacgtg
     3121 ctgatgagca gcccgcagga atggtactac ctgctgcccc aattgcgcca gacgatccgg
     3181 caaatcatgg ggcgcgatct gccggttgcc atcaccgaaa tcaatgccac gccttccagc
     3241 gcccagcatc cgagcattgg caacgaggcg ttgtggtggg ccgatacact cggtacgctc
     3301 atgaacgagc aggctgacta cgtgtcctac ttctcggcgc agggcgtccc gcagccctat
     3361 cccctgttca ccggcgatca agcgccgcag cagaccgcga cgtaccgcgt cttccagttg
     3421 ttctcgcatc tccagcgcaa cctgataccc ttgcaggtac agcacgaccc ggtcgatgcg
     3481 tttgcgacgg tggacaacac gcgccagacg atcagtatca tgttcgtcaa caaatcagcc
     3541 ggtaatcaga cggcgcaaat cagttcgctg aaccagtttt tcggcttcag cccctggcag
     3601 tcgcaggagg tcagcatcgc cggctatagc atcgtggtga ttacgctgca tcgcaacggc
     3661 ggcgcgcaag cctacagcta tgtcgtacca accgccgata ccacagcaat cacgcccttg
     3721 aaacaaactg tgtgcggcag caaatatgac ccgctcgcgt tcgccacccc atgttgatag
     3781 agaggaccaa tcgtggcact gacatatatc gggtatttct ttcttagcat ctacctgctc
     3841 attattctgc tgatggtctt ccaggctgtc ttcaatattc gcttgcgcct tttcatctgg
     3901 gaaacgccgg aacatgctca tttgaaccat gctccgacga tctatcgtga cccgtgtctc
     3961 tccttcacga tcctcctgcc ggccaaaaac gaggaggatg tgtaccgcga gacgcttcag
     4021 aaggtctacg atctgaacta tcccagagaa ctaatgcaga tcgttgccat ctgccgcgag
     4081 gatgaccccg gcacaatcgc ggaagcgcag gctaaaatcg acgaactcgg cgacccgaat
     4141 gtgcagttgc tcatttataa tgactacccc atcagcaagc cgcatggcct gaatctgggc
     4201 ctgcaggtcg cgcgcggcga tattgtgacg atcttcgacg ccgaagacga gccacatccc
     4261 gatatcctca atatcatcaa taccactatg ctgaatgaag atgtggacgc agtgcagagc
     4321 ggggtgcaac tgatgaacca taatacgaaa tggttctgtt tcttaaacgt acttgaatac
     4381 ttcttctggt ttaaatcgtc catgcacttc ttcgcccgca tcggcatgac gccgctcggt
     4441 ggcaataccg tctttgtgcg gcgcgaactg atggaacagc tcggcggctg ggacgaaacc
     4501 tgcctgaccg aggatgccga tctcggcatt cgcctgagcc tctctcacgc gcgcatccgg
     4561 atcatctacg aagacgagtt tgtcacgcgc gaggaaacgc cgcacaccat cgaacaattc
     4621 atcaagcagc gcacgcgctg gaaccagggc tttatccaga tcctcttcaa gtgggaatgg
     4681 ttgaagctgg agacgctttc ccagcgcctg ctggcatttt atgtcttaat cctgccggaa
     4741 gtgcaggcgc ttttcgcgat cctggtaccc gtcgccatca tcatgttctt taccgtcaag
     4801 ttcccgctct ggctggccat gttgaccttc gcgccgctct attgcttcgt gctggatgtc
     4861 ttcatggatg ttgcgggatt gcacgagttt ttgaaggctc acgggcagaa atggagctgg
     4921 cgagaagcct ttattctggt aatggccttt ttcccgtacc agtggatact gagcctcggc
     4981 gcactgcgcg ccgtttaccg cgcgacacgc ggcgcaacca actgggagaa aacggcgcat
     5041 attggacagc accgcgagaa aatcgcggcg tgaatatcat agttaagaga gtaccatgac
     5101 agtttcctat acaccgcaac aagatcaaga cccacagcaa ccagcgcgac agacgccagg
     5161 cgcagacgct cccggcacag cgcggcagcc cggctctcca accgacgagc cgacgagggt
     5221 tgttgagcct atccggcgga agattgtcgt gcgctctcct gtcacccctt catcaggtat
     5281 gcgctacttc gcttctgacc aggcagacgg cccgcagagc caacctttga caggccagcc
     5341 gctgactccc aacgaacccc ggccccagaa tggcgtggca gacgtggatc ggctgcctac
     5401 cagcgcgctg cccgccccat ccgctccgcc agtcgatgga cactatcgac aactcggtca
     5461 gcaagtacaa aacatccgcc aggatatggc gcaggtgcgc cagccctctg ctctgcgcaa
     5521 cggcgcgagc aatagcgtga tagatcgtat cgctactgct gtctcgcctg cctctacgcc
     5581 ttctgcccct cccagtcgcg ctgcaaccgc ctcatcgaga gggactgctg ccaggccaga
     5641 ggtcgagaac ggcaatatta tcctgtatcg cacgcacaac ttcttcttgc gtaccgcctt
     5701 ccggtcaggg caaacgggca acccgctgcg gcgaccgtcc gggcatacga gcgttatgcc
     5761 gcgtgtcatg cccgatcagg agaagcgggt cgcggccagc gacacacacg tcatgcccag
     5821 catcatcgtg aatcccggca agcaggccag ggctattccc ataccctcct ggtcagaggc
     5881 catcctggtg atgttcgcat tgatagggac ctgcgtggca catgcctaca acatgttcaa
     5941 cttcccgcgc tacgagctgg atgaaggcac ctacatgtcg tatgcctggg ccattttgca
     6001 cggcaacatc gcctccgaac catacggcta cggccacccg ccgctggggt ggattcagat
     6061 cgcgggctgg atacaaatga cgggcggctt cttcaccttc ggcactgcta tcaacagtgg
     6121 gcgcgtcctg atgctgctgt acgcgcttgg cagcgcgctg ctggtctatt tgatcgtgcg
     6181 ccgcatgagc ggcagcgcca gcgcgggtct gctggccctg gtgatcttct cgttttcgcc
     6241 cttgagcatc acctaccagc gccaggtctt cctcgataac atagcggtct tctggctgct
     6301 gatgtcgttc tacctgctcg tcgtgggcaa cagccgcctg aaatttatca tcggcgcggc
     6361 gatcagcttc ggcgttgcct ttctgtcaaa agaaacctct gccgtctttt tccctgctat
     6421 tgtctacgcg gcctggttgt actccacgaa attccagcgc aagttcagta tgaccgcctt
     6481 catttataca atcatggcgg tctgctcaac ctgggtgctg atggccgtgc tgaaaggcga
     6541 actgtttccc tatacctggc atctgccctg ggatacgcac ccgcacttga gtatgctcga
     6601 taccttcgtc ggccaggcgc agcgcggagt ggaaagcggc agcctgttca gcagcttcca
     6661 gatctggcag gagaacgacc cggtcttcat catcctcagt attgccgcgc cagccttcaa
     6721 cctgctctat ggctggtgga accgcaagca cctcatgctc gcgctgatgg ccgcgctcta
     6781 ctggctgctg ctggtgcgcg gcggccaggt gctgtcgttc tatgtcatca tgctcatccc
     6841 ggtcacggct cttaacaccg ctatggcgct caacgctatc ctgaactggg gaagcagaat
     6901 tggtcactgg cgctggctga atatggcctt tactgtcacg cgcgcgctgc tgctgctcgt
     6961 cgccctggcc gccatcctgc cctacgacat tcaggagacc agcttccgct tctaccagca
     7021 cccgacatct gcccaggagc aggcgatggt ctggattcgc gatcacgtct cgcgcgactc
     7081 cttcatcgtc atcaacagct acctgtacct cgacctgcgc ctgcccggtg gcaccggcgt
     7141 gggcgatagc gcacccttcc cacatgccga ggtctactgg gaggttgcct acgacccgac
     7201 cttgcacgac aaaggcgggg cgctccagga taactgggat cgcatcgatt atatcgtcgc
     7261 cgactcggaa atgctgaacg acatcaagac ctacggcggt cctatggatc tcatcaaaac
     7321 agcatatgag cattcc
//
LOCUS       DASZAK010001168         2953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_670054, whole genome shotgun sequence.
ACCESSION   DASZAK010001168 DASZAK010000000
VERSION     DASZAK010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670054"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VGT44_20700"
     CDS             <1..649
                     /locus_tag="VGT44_20700"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583292.1"
                     /translation="RVEYQPKTATRLVHLPLIDNEAGLIWFANEGAIEFHLWSSHVPD
                     LTKPDQAIFDLDSGETASFQDLREAALRLHDRLEQAGVKGYPKTSGGRGLHIFVPLAA
                     GYTFERVRPWVKAVGQQLATTYPDLISIAHGPTHKGGRVTIDYAQNSVGRNTAAPYTL
                     RASARHPTVSTPLTWEELDAGNVHPADLTPQVVLERVQRNGDLFAPVLQADQHIA"
     gene            1056..2885
                     /locus_tag="VGT44_20705"
     CDS             1056..2885
                     /locus_tag="VGT44_20705"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV2583293.1"
                     /translation="MSLSSRSKVGFLLSLISIVAIVGIIMVNLAFQRPAAHAFNADVL
                     VSNGSPSTPFSQNKQNEPALAVDANHPNILVAGSNDEIDMEACNAGTDNTCPFTPGVG
                     TSGVYFSFDSGTTWTQPTYQGLTGRGCLGVVGDTDPPCTPQVGSIGTLPHYFENGLVS
                     DGDPALAFGPSPDASGHFSWSNGSRLYYANLTSNLPGTGTFKGFEAVAVSRTDNVTAA
                     ATGDATAWMNPVIITKQSSTTFSDKEQIWADNAASSPFFGNAYLCNASFRSDSHGNAS
                     PQPLLVATSTDGGATWTQKQVTSASNNPFNTKQGFGRSGCTVRTDSHGVVYVFANQFA
                     VGMPAHSSHLMIKSFDGGKSWTRPVNIGLAVDNCFLVQFDGSGFRCVMDGVGGARSDL
                     SASPSVDIANGAPTGAGATNEILRTWADGRDGVNHEHVFVSFSTDGGNFWSAPTATET
                     AGDRGYYTAIAISPSGTDAYLVYNAFTTPLRNDTTSPRTLVGVVKHADIDATGVPGAW
                     SELHRGAAGDPRGSAQNNLWLEFLGDYVYAVATNTYGAGVWNDTRNAADCPAIDNWRA
                     AAQAAVLNGTTVPVKPAPQQDCGPNNTFGNSDIFGGSFADPTP"
BASE COUNT          619 a          913 c          812 g          609 t
ORIGIN      
        1 gagagtcgag tatcagccta agactgccac gcgcctggtt cacttgccgc tgatcgacaa
       61 tgaagcgggc ctgatctggt tcgccaatga aggggccatc gaattccacc tctggagttc
      121 acatgtcccg gacctcacta agcccgatca ggccattttt gatctggatt ccggcgagac
      181 cgcgtccttc caggatcttc gagaggccgc gctgcgcctg catgataggc tcgagcaagc
      241 aggagtaaag ggatatccga agaccagcgg tggacgcggc ctgcatatct tcgtgccgct
      301 agcagcaggg tataccttcg agcgcgtgcg cccctgggtc aaagcggttg gacagcagct
      361 cgctaccacc tatccagatc tgatttcgat tgctcatggt ccgacacaca agggaggtcg
      421 tgtgacaatt gactacgcgc agaacagcgt aggccgcaac acagcagccc cctataccct
      481 ccgcgccagc gctcgacacc cgacggtttc cacccctctc acctgggaag aactggatgc
      541 cggcaacgtt catcccgcag atctaacacc tcaggttgta cttgagcgag tccagcggaa
      601 tggcgatctc ttcgcaccag tactacaggc ggatcagcac atcgcatagc ttttcagcgc
      661 atcgactcgg gtagtgggcc gggcgccctc aagggatagt gttggcgaat gaccgtgtgc
      721 tttcccacgg cgagatggga aagcgaccag ggcaggggcg aggacaagcc atcgccccat
      781 ggacgagcct gccaggccgc tacgtcagca tagtaggggc gaggcttgtc ctcgccctgg
      841 ggaatgaaca gagaaatctc accctcaatc gagaaatctt aaccaatcct caacctgatc
      901 ataaccctat tgctcttgta tctatcatgc ctgctcttat gattgctatg atgcgcgatt
      961 cttctctaat cttagagaag agcattgcat gagctctcgt ctcaagtaat gtgcatttga
     1021 acattgagca gttttcttga aagtgaggtt cttgtatgtc tctctcttca cgctcaaaag
     1081 taggtttcct cctatccctc atcagcattg tcgccattgt gggcatcatt atggtgaatc
     1141 tagcctttca acgtcctgct gcccacgctt tcaacgccga tgtcctggtc agcaacggga
     1201 gtccttctac ccctttctct cagaacaaac agaacgaacc agcattggca gttgatgcca
     1261 accacccgaa tatcttagta gctggctcca acgacgagat cgacatggag gcctgcaatg
     1321 ccgggaccga caacacttgc ccattcactc ctggtgtagg cacatcgggg gtctacttct
     1381 catttgacag cggtaccacc tggactcaac ccacgtatca gggcttgacc ggtcgtggct
     1441 gcctgggagt cgtcggggac accgacccgc catgcacccc ccaggtagga tcgattggta
     1501 cgctgcccca ctattttgaa aacggactgg tctccgacgg tgaccccgcg ctggcgttcg
     1561 gccctagccc tgatgccagt ggccatttct cctggtctaa cggctcgcgt ctctactacg
     1621 cgaacctcac ctcgaacctc cctggcacgg ggacatttaa gggctttgaa gccgtcgccg
     1681 tctcgcgcac ggataatgtg acagcggcag caacgggtga tgcgactgcc tggatgaacc
     1741 cggtaatcat caccaagcag tcatcaacga ccttcagcga caaggagcag atctgggctg
     1801 acaatgccgc gagtagtccg ttctttggca atgcctacct ctgtaacgct tcgttccgca
     1861 gcgatagcca tggcaatgcc tcgccacagc ccctgctcgt tgccacctcc acggatggcg
     1921 gcgcgacctg gacccaaaag caggtcacat cagccagcaa caaccccttc aacaccaagc
     1981 agggcttcgg gcgatccggc tgcaccgtcc gcaccgacag ccatggcgta gtctacgtct
     2041 tcgccaacca gttcgctgtt ggcatgcccg cacacagctc tcacctcatg atcaagtctt
     2101 tcgacggggg caagtcgtgg acacggcccg tgaacatcgg gcttgccgtt gacaactgct
     2161 tcctcgtcca gttcgacgga tctggcttcc gctgcgtgat ggacggcgtg ggcggcgcgc
     2221 gctccgacct ctcggcatct cctagtgtgg atatcgccaa cggtgcgccg acgggtgccg
     2281 gtgcaaccaa tgagatcctg cgcacatggg ctgatggtcg tgacggggtc aaccacgagc
     2341 acgtgtttgt gagcttctcg accgatggag ggaacttctg gtcggctccc acggcaacag
     2401 aaaccgcagg tgaccgcggc tactataccg ccatcgctat ctcgccgagc ggaacagatg
     2461 cctacctcgt gtataacgcg ttcaccacgc cattgcgcaa tgataccacc agcccccgca
     2521 cgctggtagg cgtagtgaag cacgcggaca tcgatgcaac tggagttcct ggcgcgtgga
     2581 gcgagctgca ccgtggtgcg gccggagatc cacgaggttc ggcccagaac aacctgtggc
     2641 tggagttcct cggcgactat gtgtacgcgg tcgcgaccaa cacctacggt gcaggggtct
     2701 ggaatgacac ccggaacgcg gcggactgtc cagcaattga caactggcgc gccgcggccc
     2761 aggctgccgt gctgaacgga accactgtgc cggtgaagcc cgctccgcag caggattgtg
     2821 gcccgaataa caccttcggc aactcggata tcttcggagg gtctttcgcc gatcccacac
     2881 cgtaagccag gttggatcgg tagacctgag cgtttcgcca gggatgtcct tcaggcatcc
     2941 ctggtacgtg aat
//
LOCUS       DASZAK010001169         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_491424, whole genome shotgun sequence.
ACCESSION   DASZAK010001169 DASZAK010000000
VERSION     DASZAK010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491424"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VGT44_20710"
     CDS             <1..506
                     /locus_tag="VGT44_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEV2583294.1"
                     /translation="TVVLKLFSIVRPRLAYFGQKDAQQVAVIARMVTDLNLPVQLRIL
                     PTVREADGLAMSSRNAYLNKEDRLAARILYRALEAGRAAFEEHAGGPASLASDAMKAV
                     LASEPLARLDYGEVRDPSSFLRQEMLRTPAVLVIAAHVGPARLIDNFLLRADGIWDTG
                     VFVSTAL"
     gene            541..1395
                     /gene="panB"
                     /locus_tag="VGT44_20715"
     CDS             541..1395
                     /gene="panB"
                     /locus_tag="VGT44_20715"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEV2583295.1"
                     /translation="MREKVTVPAIRARKGGAKIKVVTAYDAPTAHIADNAGADIILVG
                     DTLAHVVLGYEDTLPATVDIMIHHTAAVARAKPDALIVGDMPWLSYHLSVEDAVRNAG
                     RLIREGHAGCVKLEGGRKRLPVVEAILAAEIPVMGHLGLTPQSINTFGGYRVQGRQAE
                     VAREMLEDARALAAAGIFALVLEGVPDVLAQLITASVPVPTIGIGAGPHCDGQVLVFH
                     DVLGMHNDHLARFVRQYAHLADTATEALQRFFEDVQSGAFPSDAESYHMSEEAARELR
                     EMLEQIDG"
     gene            1528..3504
                     /locus_tag="VGT44_20720"
     CDS             1528..3504
                     /locus_tag="VGT44_20720"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEV2583296.1"
                     /translation="MNVQASEPSSMRYRVAAIQYEPALGEKERNVSDLLRLTEEAARH
                     GARLIVHPEMATTGYCWLSREEIAPFVEPLPGPTTDRFSQLAAQYDCYIATSLPEVDL
                     TSGVYYNSLALIGPAGLIGVYRKVHSYISEPRWARDGDLGFPVWDTPLGRLGGVICQD
                     ASFFESTRLLALRGADVLLFPTNWLDEKCPSSWWMARAFENGCYFIAADRYGLERGVQ
                     FSGGSCIINPDGTLQAYLDNGEGIVYGDVDLLRSRDKRWGPASLEPVGNRIEDRRPAE
                     YITLTHNTYLWEPLMFHGLYDLGELPPGQLSCVGIIQTDLQDFHPLQPQSQISAQTSS
                     LELLGGMLRATLRDHEPTRPDVLALPELMLPRPFAGQGDVAAMQSHAIEIPGSETEAL
                     TALAAEFQIALVVGVAERAYEQGTMGGAPTYYNTVLLIDPEGVYGKYRKLHLTASDRR
                     WASPGNLGLPTFDTPAGRIGLATGYDVLFPETLRVLAGKGADLVCAPTYLNFPDPLGL
                     PPSAIRYKVPMQPEEYDPYHAMLWRVRADEHEVYLAVANWRGSAGGLRANGQSGIFSP
                     SCSRYPWNEVIAEDEPDADGQPALMMMTIDTREQRTGRRSTATLRYAPGEVAGSLTGE
                     LDYDRRETIPGNAVRGKPLLRKRVPHWYLDLIKK"
BASE COUNT          689 a         1122 c         1127 g          700 t
ORIGIN      
        1 cgacggtcgt gctgaaactc ttctccatcg tgcgcccgcg cctggcttac ttcgggcaga
       61 aagacgccca gcaggtagcg gtgattgcgc gcatggtgac ggatctgaac ctgcccgtcc
      121 aactgcgtat cttgccgacc gtacgcgaag ctgacggcct ggcgatgagc agccgcaacg
      181 cctatctcaa caaggaagat cggctggccg cgcgtatcct ctaccgtgcg ctggaggctg
      241 gccgcgcggc gttcgaggaa cacgcgggcg gccctgcatc gctggcgagc gacgcaatga
      301 aggcggtctt agcgagcgag ccgctcgcgc ggctcgacta cggcgaagtg cgcgacccgt
      361 ccagctttct gcggcaagag atgttgcgca cccccgcggt gctggtgatt gccgcgcacg
      421 tgggaccggc gcgcctgatc gacaattttc tcttacgcgc cgacgggatc tgggatacgg
      481 gtgtattcgt ctcgacggcg ttataagctg gcactcatgt tggaaaggaa ctgatcgacc
      541 atgcgagaaa aagtgaccgt cccggccatt cgagccagaa aaggtggggc aaagataaag
      601 gtcgtcaccg cctacgatgc tcccaccgct catatcgctg ataacgcggg cgcggatatc
      661 atcctggtcg gcgatacact tgcgcatgtt gtgctgggtt atgaagatac gctgcctgcc
      721 accgttgata tcatgattca ccacacggca gccgttgctc gcgccaaacc ggacgcgctg
      781 atcgtaggcg acatgccctg gctcagctac catctctcag tagaagatgc cgtgcgcaac
      841 gctggtcgcc tcattcgtga aggccacgcc gggtgcgtca agctggaagg cgggcgcaaa
      901 cgcctgccgg tggtcgaggc catcctggcc gccgagatcc ccgtgatggg ccacctgggc
      961 ctgacgccgc aatccatcaa tacctttggc ggttatcgcg tgcagggcag gcaggcggag
     1021 gtggcgcggg aaatgctcga agacgcgcgc gcgctggctg ccgccggtat cttcgccctg
     1081 gtgctggaag gcgtccccga tgtgctggcg cagttgatta ccgcctcggt cccggttcct
     1141 actatcggca tcggcgcggg tccccattgc gatggccagg tgctggtctt ccacgacgtg
     1201 ctggggatgc acaacgacca cctcgcccgc tttgtgcgcc agtacgccca tttggcggat
     1261 accgcgaccg aggccttgca gcgcttcttc gaagatgtgc aatccggcgc gtttccctct
     1321 gacgcggaaa gctatcatat gagcgaagag gcggcacgag aactgcgaga aatgctcgag
     1381 caaatcgatg ggtaaagtag ccatgccaga ttggtgatta tcgttacttt ccccattgac
     1441 gcttttcatc cctggtggta tggttattcc agcctcaacc attgagtcgc ccgaaggtta
     1501 tcaaacggac aacgaggggg aataaccatg aacgtgcagg catcggaacc ctcttctatg
     1561 cgctaccggg tcgcggcgat ccagtacgaa ccggcattgg gcgagaagga gcgcaacgtc
     1621 agcgatctgt tgcggctgac cgaagaggct gcgcggcacg gcgcgcgact gatcgttcac
     1681 cccgagatgg ctacgacggg ctactgctgg ctctcgcgtg aagaaattgc cccctttgtt
     1741 gaacccctgc ctggtccgac tactgaccgc ttttcgcaac tcgctgctca atacgattgt
     1801 tatatcgcca ccagcctgcc cgaagttgac ctgaccagcg gtgtctacta caacagcctg
     1861 gcgctgattg gaccggctgg attgatcggc gtctatcgca aggtgcattc ctatatctcg
     1921 gagccgcgct gggcacgcga tggcgacctg ggttttccgg tctgggacac cccgcttggc
     1981 cgcctgggcg gggtgatctg ccaggatgct tcgttcttcg agtcaacgcg cctgctggca
     2041 ctgcgcgggg cggatgtgct gcttttcccc accaactggc tcgacgagaa atgccccagc
     2101 agctggtgga tggcgcgcgc cttcgagaac ggctgctatt tcattgccgc cgaccgctac
     2161 ggcctcgaac gcggtgtgca gttcagcgga gggagctgta ttatcaaccc cgacggcacg
     2221 ctccaggcgt atcttgataa cggtgaaggc atcgtctatg gcgatgttga cctgctgcgc
     2281 agccgtgaca aacggtgggg gccagcgagc ctggagccgg tcggcaaccg tatcgaagac
     2341 cgccggcctg ccgaatatat taccctgacg cacaacacct acctgtggga acccctaatg
     2401 tttcacggcc tctacgacct gggcgaactg cctcctggcc agctctcatg cgtcggcatc
     2461 atccagaccg acctgcaaga ttttcatccg cttcagccgc aatcacaaat aagcgcgcaa
     2521 acgagcagtt tagaactgct gggcgggatg ctgcgcgcca ccttgcgcga ccatgaaccg
     2581 acgcgccctg atgtgctggc gctgcccgaa ctgatgctgc cgaggccgtt cgccgggcaa
     2641 ggcgacgtgg cagctatgca atcccatgcg attgagatac ctggctcgga aaccgaggcg
     2701 ttgaccgcgc tggctgccga gtttcagatt gcgctcgttg tgggcgtggc ggaacgagcc
     2761 tacgagcaag gcacgatggg cggcgcacca acctactata atacggtgct gctgatcgat
     2821 ccagagggcg tctacgggaa gtatcgcaag ctgcacctga cggccagcga ccgccgttgg
     2881 gccagccccg gcaacctggg cctgccaacc ttcgatacgc ctgccggtcg catcggcctg
     2941 gccaccggtt acgacgtgct gtttcctgaa acgctgcgtg tcctggcggg caagggcgcg
     3001 gacctggtgt gcgcaccgac ctacctcaat tttcccgatc cgcttggcct gccgccctcg
     3061 gccattcgct ataaagttcc gatgcagccc gaggagtacg acccatacca cgctatgctg
     3121 tggcgcgtgc gcgccgatga acacgaggtc tacctcgctg tcgccaactg gcgcggcagc
     3181 gccggcggcc tgcgcgccaa cggccagagt ggaatatttt cgccttcatg ttcgcgctat
     3241 ccctggaacg aggtgattgc cgaggacgag ccggatgctg acggacagcc agcgctgatg
     3301 atgatgacga ttgacacgcg cgagcagcgc acaggccgcc gttcgacagc gacgctgcgc
     3361 tacgcccccg gcgaagtggc cggttccctg acgggggaac tcgattacga ccggcgtgaa
     3421 acgatccctg gtaatgcagt gcgcggcaag cccctgctgc gcaagcgtgt gccgcactgg
     3481 tatctcgact tgatcaaaaa atagtaagga gaggttaccc ctcatgagac tcaaggacaa
     3541 agttgcgctg attaccggct ctgctggcgg catgggccag gccgccgccg aactcttcgc
     3601 gggcgaggga gccagtatta tcgtgaccga tatcgtcg
//
LOCUS       DASZAK010001170         3516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_349236, whole genome shotgun sequence.
ACCESSION   DASZAK010001170 DASZAK010000000
VERSION     DASZAK010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349236"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2660)
                     /locus_tag="VGT44_20725"
     CDS             complement(<1..2660)
                     /locus_tag="VGT44_20725"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEV2583297.1"
                     /translation="MPEERKLVTILFSDVTGSTALGESLDPEDVRTLMGRYYSHARDV
                     ITHHGGTIEKFIGDAVMAVFGLVQTHGDDAERALAGALALREAVDGDEILGLAFQLRM
                     GVNTGEVVASSDPSSGDFLVTGDAVNVAARLQQHAQPGEIVAGERTVSAAQTAFCFDE
                     PRLVEVKGKKLPLRVYPLKGAQTTRKVFRPPFVGRKQDLMQLMLLRERILEEERPQLV
                     SIVAPAGTGKTRLLEEFLKRLDPDEGFQVAMVRCLPYGQTLTYWPLRGLLDGLLLGEP
                     IAKSTVERVFAGGGYKEDDAARLADLVLSTLGIEGEGMAGAGDRESIFTAWRLLIEVF
                     AKQAPRVLIFEDLHWASDSMLDLVEHIISVHIQARMLLIVLSRPELLDRRPTWGGGRQ
                     NFTALALQPLSTRQTQELIKQLTTDLPDSLRELIVERSGGNPFFALELVRGQVERERA
                     GESAPLDVLPDTVHAAVQARLDMLLKQERTLLQVASVATRAIRSPMLRAMLGDASAQQ
                     IDEALASLVERDLLVPVDAETYTFRHILIRDVAYSTLSRAERIRLHASIAAWLEAAAA
                     DHLDEYAELIAYHYREAVMLAKQSAVPKPMAVETAKAVHYLERAGELAARAGAFAETK
                     MHLRNALSLASESDTMRLNEKLGDCLVWGDTVVDAYSAALERWRAEGQHDALTGARLM
                     RKLLMAYNKTNKQPERAVLDALWNEAEQLVVQANNEAELWRFRTDSLVRRLLQALTRG
                     AIDEAESRDALATCQAAADFFEAQGDWEFLDEVLDIWIGFLGTIGAHVDALAVSQRRL
                     ATPGITTRERGDATGGLASIYFLLGDYDRCIATACEAVAALHPGEPIEYFAGAVSVAM
                     WATYASGQWEEATALLKTLEQIWERLQLRPG"
     gene            complement(2653..3327)
                     /locus_tag="VGT44_20730"
     CDS             complement(2653..3327)
                     /locus_tag="VGT44_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583298.1"
                     /translation="MSSQPTPTTPPQARSSPLMPSPRPPRFANVRGWLRGRWGRIVVP
                     IIALILGFALGITALFVYGLSGEGTVMGTPAPGKQDIVVEADKAFLTNLVKYNLQHAG
                     MPGTIQNVTVVLATGDQMTISGDDTFGVLGLGVTKHFQLVVQPYIASCVLQVHVVHAD
                     LSGIPVTGLAQTFESQTNQQLAQKPSGLPKDFQYCASSVRTEPGGIFVAYSATPLAMN
                     GAVFYA"
BASE COUNT          770 a         1069 c          947 g          730 t
ORIGIN      
        1 ccaggtcgta gttgcagccg ctcccagatt tgttccagcg tcttaagcaa agccgtagct
       61 tcttcccatt ggcctgatgc gtaggttgcc cacatagcaa ctgagactgc tcctgcgaag
      121 tactcgatag gttcgccagg atgcagcgct gccacagctt cgcaagcggt agcgatacat
      181 cgatcgtagt caccaagcag gaagtagatt gatgccagcc cgcccgtggc atcgccgcgc
      241 tccctggtag tgatgccggg agttgcgagg cgacgctgac tgaccgccaa agcatcaaca
      301 tgcgccccaa ttgtccctaa aaatcctatc cagatatcca ggacctcatc gagaaattcc
      361 caatctcctt gcgcctcgaa aaaatccgca gcagcctgac aggtcgctag agcatcccgc
      421 gactcggcct cgtcaatggc tccgcgcgtt agtgcttgca gtaagcgcct gaccagggag
      481 tcagtgcgga agcgccacaa ttcggcctcg ttattcgcct gcacgacaag ttgttcggcc
      541 tcgttccaga gggcatcaag cactgctcgt tccggctgtt tgttggtctt attataggcc
      601 atcagcagct tgcgcatcaa cctggcgcct gtcagcgcat catgctggcc ctccgctctc
      661 cagcgttcga gcgcggcgct ataggcatcg acgaccgtat caccccatac aaggcaatcg
      721 cccagtttct cgttgagtcg catcgtatcc gattcggacg ccagggaaag cgcgttgcgt
      781 agatgcattt tcgtctcggc aaacgccccg gcgcgcgcgg caagctcacc ggcgcgttcc
      841 aggtaatgaa cagccttcgc tgtctcaaca gccatcggct ttggtacggc agattgtttc
      901 gccagcataa ccgcctcgcg ataatgatag gcgattaatt cggcatactc gtccaggtga
      961 tcggcggcgg cggcctcaag ccaggccgcg atgctcgcat gcaggcgaat gcgttcggcg
     1021 cgcgaaagtg tgctgtaggc cacatcgcga atgaggatat ggcggaacgt ataggtctct
     1081 gcatcgaccg ggaccagcag atcgcgctcg accaggctag caagcgcctc gtcgatctgc
     1141 tgtgcgctgg catcgcccag catggcgcgc aacatcgggg agcgaatggc gcgggtcgct
     1201 accgaagcca cctgcaacaa cgtcctctcc tgtttcaaga gcatatccag ccgcgcctgc
     1261 actgccgcgt gaacggtatc gggcaacaca tccagcgggg ccgattcacc cgctcgctcg
     1321 cgttccacct ggccgcgcac gagctcaagc gcgaagaaag ggttgccgcc agagcgctca
     1381 acaatcagct cgcgcaggct atcgggcaag tctgttgtaa gctgtttgat aagctcttgc
     1441 gtttgcctgg tcgaaagggg ttgtaatgcc agagcagtaa agttctgccg accgcctccc
     1501 caggtgggac gtcgatcgag caattccggg cggctcaaca cgatcaagag cattcgggcc
     1561 tggatatgca cgctgatgat atgctcgaca aggtcaagca tactgtcact ggcccagtgc
     1621 agatcttcga agatcaggac gcgcggagcc tgcttcgcga agacctcgat caaaagccgc
     1681 cacgcggtaa agatgctttc tcgatcaccg gctcctgcca tgccttcacc ttcgataccc
     1741 agcgtcgaaa gcacaaggtc agcaaggcgt gcagcatcat cctctttata accacctccc
     1801 gcgaaaacgc gctctaccgt agattttgcg atcggttcgc cgagcagcag gccatccagc
     1861 agaccgcgca ggggccagta ggtgagggtc tgaccgtagg gaagacagcg caccatcgcc
     1921 acttgaaatc cctcatcggg atcaagtcgc ttcaagaact cttccagcaa acgtgttttc
     1981 cctgttccag caggcgcaac gatcgacacc aactgcgggc gttcttcttc caggatgcgc
     2041 tcccgtaaga gcatgagttg catcaaatcc tgtttgcgcc ctacaaacgg gggccggaag
     2101 accttgcgcg tggtctgcgc tcccttaagg ggatagacac gcagtggcag ttttttgcct
     2161 ttcacctcca ccaggcgcgg ctcgtcgaag caaaatgccg tttgcgccgc gctaactgtg
     2221 cgctctccgg cgacaatctc ccccggctgg gcgtgttgtt gcaagcgcgc cgctacattc
     2281 acggcgtcac ccgtcaccag aaaatcgccg ctggatggat cgctacttgc caccacctcg
     2341 ccggtattga cgcccatccg taattggaac gccagaccga ggatttcgtc accatccact
     2401 gcctcgcgca gggccagcgc ccccgccagc gcgcgctcgg cgtcatcccc gtgcgtctgg
     2461 accagcccaa agacagccat caccgcgtcg ccaataaatt tctcgattgt gccgccatga
     2521 tgggtaatca catcgcgcgc atggctataa tagcgcccca tcaatgtccg cacatcttcc
     2581 gggtcaagcg actcaccaag cgcggtcgaa ccggtcacat cagagaacag gatcgttacc
     2641 agtttgcgtt cttcaggcat agaatactgc tccattcatt gcaagtggcg tagccgagta
     2701 ggctacaaag atgccacctg gctcggtgcg cacactgctc gcgcaatatt ggaagtcctt
     2761 tggaagacca gatggtttct gggcaagctg ctggttggtt tgcgattcaa acgtctgcgc
     2821 caggccggta acgggaatgc cactgaggtc ggcgtggaca acgtggactt gcagcacaca
     2881 agaagcaatg tagggctgga cgacgagctg aaaatgcttg gtgacaccca gacctaacac
     2941 accgaacgta tcatccccgc taattgtcat ctggtctccc gtcgccaaaa cgaccgtgac
     3001 attttgtatc gtccctggca ttcctgcgtg ttgcaggttg tatttcacga ggttggtgag
     3061 aaaagccttg tcggcctcca caacgatgtc ttgcttgccc ggcgcgggag tacccatgac
     3121 cgttccttca ccagataggc cgtacacgaa cagcgcagtg atgcccaggg caaacccaag
     3181 tatcaaagcg atgattggca cgacgatgcg tccccatcga ccccttaacc acccacgcac
     3241 gttcgcaaaa cggggagggc gaggtgacgg catcaaggga ctcgaacgcg cttgcggcgg
     3301 tgtggtaggc gtaggctgag aactcataaa actaagctcc tctaaattgg cttcatctat
     3361 attatatgct tctcaatgag aacgcaagct ttcgttcgtt ttgtgcaagt tgcacaaata
     3421 cctgacaagt tcttttagac aaactaactg tagtattcgc ctcctttacc ctttacgata
     3481 gtaggagaaa ttggatagat cagtggtcaa acgccc
//
LOCUS       DASZAK010001171         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_598825, whole genome shotgun sequence.
ACCESSION   DASZAK010001171 DASZAK010000000
VERSION     DASZAK010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_598825"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1111)
                     /locus_tag="VGT44_20735"
     CDS             complement(<1..1111)
                     /locus_tag="VGT44_20735"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEV2583299.1"
                     /translation="MVKHLIRRSIVGAPVSLLLITMLLFLSGQSLTAVNAASGFSFKT
                     FELSSTPGVNCPNAANTCTNGAAEPQIRADKAGHFYASSENGLGGGTEAWNSTNGGKN
                     FLALVSPNAGSSTNNTGFAPGGGDTDVAVAPVKNTSGQYNVYIASLSLANVDVSTSTD
                     GGKTWALNLTSATIPGDDREWIAADGASKVCISYHDAATFNINVNCSSDAGSTFTQLG
                     EAFDTNHLWLAQNNSTGNLVIDPTNHYVYQTFSGIANSGEVMCSQQGTCGYHVVWVAV
                     STDGGKTFTDHMVYDNPTTTVSYGHQFVNLSIDREGNLYSVYSDNHNTFYSFSTSHAT
                     TWSAPVQVNGAGAATAIMPWSVANNAGHLDIVFYGT"
     gene            complement(1326..2993)
                     /locus_tag="VGT44_20740"
     CDS             complement(1326..2993)
                     /locus_tag="VGT44_20740"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV2583300.1"
                     /translation="MAKASVPSGLFVRQASGLVRDVSMANALFFNIAAFVGTAIGWAV
                     AFYALGPEWQGLGISAFAWMAILTGIFCYFLGMIFASLTTAMPRSGGDYVFTSRIISP
                     FLGWIESWTLVGSALTIIGFEIIVATHNVQLTAILLGVAFPGSAFANAPNWLQDNTSK
                     AIVGAIILLVIFGISILRTRTFHRVVTGLTIFAIISVAISFFGAFFISSSQVDAALPA
                     LGVKSAQDLLNAASKGGWSLGSPDFSFLGLIGLAGLVAIVLFQFIGFQFSSYIAGEVK
                     GNVRRSIMFAVIGALLFAVFMNSLYQDVLGNHFGFPLVNAWGYLYANGLGAPLNGQPP
                     FTPVLATAVYPGAWPLWLIVSIGNTVLNVLLCPVYAIFLSRIVLAWSLDRQVPEWFSV
                     VNERTNAPLRVIVLAILLGAVFFVLTFFGYTLANTIFFGILLAGLTWIMPGLNAILIP
                     FRRPDLFQLTNNTGRFLGLPRLVWSGILWLIFIVPVYAAAFVYPVVQGIRAQGGAYLS
                     LSTSNTSGIGWALVFILVGIVIYFVMRFVNVSKGINVKMIYQELPPD"
     gene            complement(3074..>3702)
                     /locus_tag="VGT44_20745"
     CDS             complement(3074..>3702)
                     /locus_tag="VGT44_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583301.1"
                     /translation="RRKMIERVGEWVELAEERIGKSGVQFYMMPGNDDDFGVDGMIAR
                     SSYVVNPVGKTIALNEFHEMISFDYANHTPWHTPREWSEEEYYEHVKERASGLQDVKN
                     AVFMIHVPPYDSGLDTAPELDKTMRPKVTMGDVLRVPVGSTGVRKVIQELQPVVSVHG
                     HVHEAGGQAKIGRTTCFNAGSEANQGILRGFVFDLGKDKLERAFRVQG"
BASE COUNT          873 a         1036 c         1085 g          708 t
ORIGIN      
        1 aggtgccgta aaagacgata tcgagatgcc ccgcgttgtt agcgacgctc cacggcatga
       61 tggcggtagc tgctccagcg ccgttcacct ggacgggagc cgaccacgtg gtcgcgtggc
      121 tggtcgagaa gctatagaag gtgttgtggt tgtcgctata cacggagtag aggttgccct
      181 cacggtcaat gctcaggttc acgaactggt ggccataact gacagtcgtg gtgggattgt
      241 catagaccat gtgatcggtg aacgtctttc cgccatcggt cgaaacagca acccagacta
      301 catgatagcc acaggtgcct tgctggctac acatcacctc gccagagttg gcaatgccgc
      361 tgaaggtctg gtagacatag tggttggtcg ggtcgatcac caggttcccg gttgagttgt
      421 tctgagccag ccatagatga ttggtatcga acgcctctcc caactgggta aacgtggaac
      481 cggcatcgct gctgcaattc acattgatat tgaacgttgc ggcatcatgg taggagatgc
      541 agactttgga cgcgccatcg gcagcaatcc attcccggtc atcgcctgga atagtggcgc
      601 tggtgagatt gagagcccag gtcttgcccc catcggtact ggtactgaca tcgacatttg
      661 ccaggcttaa actggcgatg tacacgttgt actgcccgct cgtattcttg acgggcgcga
      721 cagcaacatc cgtatctccg ccgcccggcg cgaatcccgt attattcgtg ctagacccgg
      781 cattgggcga aacaagcgcc aggaaattct taccgccatt ggtggaattc caggcttcgg
      841 tgcctcctcc cagcccattt tcagaggaag catagaaatg acccgctttg tccgcccgaa
      901 tctgcggctc agccgcgccg ttggtacagg tattggcggc gttggggcag ttgactcccg
      961 gagttgaact cagctcaaac gtcttgaagc tgaagccgct ggcagcattc accgcggtca
     1021 ggctctgccc tgaaaggaag agaagcattg tgatgagaag cagggacacc ggcgctccca
     1081 caatggagcg tcgaatcaga tgtttgacca tacatcctcc ttgttatggt ctctttagct
     1141 ttgcagctat gcgtagaggg cgctgcccag gtagcagcgc gagagtagtt gccagttcta
     1201 taaactggac taatctctgt atggtaaaca cgtatgagat tacgaaaagg acttattctc
     1261 aaaaagaaaa gggtaagtag agaaatttaa tctctactta cccttgagcg gtaatgcaac
     1321 acacgctaat ccggcggcaa ctcctggtag atcatcttga cgttgatgcc cttgctgacg
     1381 ttaacaaagc gcatcacgaa gtagatcaca atgcctacca ggatgaagac gagcgcccag
     1441 ccgatgccac tcgtatttga agtggagagc gacaggtagg cgccgccctg cgccctgatg
     1501 ccctgcacca ccgggtagac aaacgcggcg gcatagaccg gcacaataaa gatcagccac
     1561 agaatgcccg accagaccag gcgtggcagc cccaggaacc tgcccgtatt gttggtcaat
     1621 tggaagaggt cgggccgccg gaaggggatc agaatggcat tcaagcccgg catgatccag
     1681 gtcaggccgg ccagcaggat gccgaaaaag attgtattcg ccagagtata gccgaagaat
     1741 gtcagtacga agaacactgc accgagcagg atggcgagaa cgattacgcg cagcggggca
     1801 ttggtgcgct cattcaccac gctgaaccac tcgggaacct gccggtcaag cgaccaggcc
     1861 agcacgatac gagagaggaa gatggcgtag accgggcaga gcagcacgtt cagcaccgta
     1921 ttgccgatgg agacgatcag ccagagcggc caggcaccag gatagacggc ggtcgcgagt
     1981 accggcgtga agggaggctg gccgttgagc ggcgcaccca ggccgttcgc gtagaggtag
     2041 ccccaggcgt taaccaacgg gaagccgaaa tggttgccca gcacgtcctg gtagagcgag
     2101 ttcatgaaca cagcgaagag cagcgcgccg atcacggcga acatgatgga gcgccgcaca
     2161 ttgcccttga cctcgcccgc gatgtacgag gagaactgga agccgatgaa ctggaaaaga
     2221 acaatggcta ccagccccgc cagcccgatc aatcccagga atgagaaatc gggcgagcct
     2281 agcgaccacc ctcctttact cgccgcgtta agcaaatctt gggcggattt gacgccaagc
     2341 gcgggcagag cggcatcaac ctgactggag gagatgaaga acgcgccgaa gaagctgata
     2401 gccaccgaga tgatggcgaa gatggtcagg cccgtcacga cgcgatggaa ggtgcgcgtg
     2461 cgcaggatcg agatgccgaa gatcaccagc aggataatcg cgccgacgat ggccttgctg
     2521 gtattgtcct gaagccagtt gggcgcgttg gcaaaggcgc tgccgggaaa ggcaactccc
     2581 agcaggatgg cggtcaactg gacgttgtgt gtagctacga taatttcaaa gccgatgata
     2641 gtgagcgccg aaccgacgag cgtccaggat tcgatccagc ccaggaacgg gctgatgatg
     2701 cgcgaggtga agacatagtc gccaccgctg cgcggcatag ccgtcgtcag actggcgaag
     2761 atcatgccca ggaagtaaca gaaaatgccg gttagaatgg ccatccacgc gaaggccgag
     2821 atgccgagac cctgccactc cgggccgagg gcatagaagg caactgccca gccgatggcc
     2881 gtgccgacaa aggccgcgat gttgaagaag agcgcgttgg ccatactcac atcgcgcacc
     2941 agccccgatg cttgccggac gaataagccg ctgggaacgc tggcttttgc cataagtcca
     3001 aaaccccttt cttaacgaga gcagcgtctg cgacaatgaa ggaagaagga gccgcgctgc
     3061 tcccaggaca agactatccc tggacgcgga aagcccgctc cagtttgtct ttaccaagat
     3121 caaagacaaa gccgcgcagg ataccctggt tggcctcgct tcccgcgtta aaacaggtcg
     3181 tgcgcccgat tttagcctgc ccaccggcct cgtgaacatg cccatgcacc gataccaccg
     3241 gctgcaactc ctggatcact ttccgcacgc ctgtgctgcc caccggcacg cgcaagacat
     3301 cgcccatcgt gactttaggc cgcatggtct tgtccagttc tggcgcagta tccaatccgc
     3361 tgtcataggg aggaacatgg atcatgaaca ccgcgttttt gacatcctgt aagccgctcg
     3421 cgcgctcctt gacatgctcg tagtattcct cttcgctcca ttcgcgcggt gtgtgccagg
     3481 gtgtatggtt ggcatagtcg aagctaatca tctcgtggaa ttcgttcagg gcgatggttt
     3541 tgccaaccgg gttgacgacg tagctggagc gcgcgatcat cccatccacg ccaaaatcgt
     3601 cgtcgttgcc gggcatcatg tagaactgga cgccgctctt gccgatgcgt tcctcggcaa
     3661 gctcgaccca ttcgcccacg cgctcgatca tcttgcggcg aa
//
LOCUS       DASZAK010001172         3874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_64874, whole genome shotgun sequence.
ACCESSION   DASZAK010001172 DASZAK010000000
VERSION     DASZAK010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3874
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64874"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(106..813)
                     /locus_tag="VGT44_20750"
     CDS             complement(106..813)
                     /locus_tag="VGT44_20750"
                     /inference="COORDINATES: protein motif:HMM:NF025636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2583302.1"
                     /translation="MRDQVDLRVRRTQANLREALIDLIEEKGFDAVTVGDIAERAMVN
                     RTTFYRHYPDKYALVTGIFEDAVNQMFREIGPLPKNLEGLLSSESKLDARAVQSYIES
                     QLAAWSAFFEHVARHARLYRVMLGKRGSSWFTVQMRDYFAQAIRSRARTLSAAGLRVA
                     DDPQAAPAEFVIISLAHWLVGMLAWWIENGMASSPQQMTLWTARFALYGYPAVLGLNI
                     PHIIGGVAPSDWLSKQS"
     gene            965..1957
                     /locus_tag="VGT44_20755"
     CDS             965..1957
                     /locus_tag="VGT44_20755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583303.1"
                     /translation="MTNTPAILAEGLSKAYGKIHALDGLDLVAEAGTVLGVLGPNGAG
                     KTTTVRILTTLIKPDRGRAEVAGLDVVKHAATVRERIGLTGQYAAIDELLTGYENLKM
                     FGQLSRLPGAVARRRAHDLLARFDLEEAAGRLVKTYSGGMRRRLDLAASLILSPPILF
                     LDEPTTGLDPRGRLALWEVIRDLAAQGTTVLLTTQYLEEADHLAHQIVVIAQGRVIAQ
                     GTASELKTQIGGERLELTVAPHSDLDAAWQAIRPYSSGEIQLDHERRHLTVPVTNGAT
                     LLAAIVRDLDGAQITLSDLALRRPTLDDVFFSLTGHAVSEAEQPEEARLQRSQR"
     gene            1954..2775
                     /locus_tag="VGT44_20760"
     CDS             1954..2775
                     /locus_tag="VGT44_20760"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583304.1"
                     /translation="MKNPVLLTREEVIPRSHFALYWTFAHAWVLAKRRLTQIPRRPDE
                     LLIATLQPTMLVLLFRYVFGGAIRVAGTSYANYLMAGVFVLASLVGSLATGIGLATDL
                     QQGLLDRFRSLPMARSAVLTGRTLADLVRNGFVVLVIWAVGLLVGFRPAGPPLAWVAA
                     TGVLLLTTFTFSWLAVLLGLVVSSPEAVQGASLVLFYPLLFGSNAFVPTSTMPDWLRA
                     FADHQPISVVIGAVRGLLLNQPDAATIGQALTWCVVLLMIFIGLSIWAYGRRTAK"
     gene            2888..3874
                     /locus_tag="VGT44_20765"
                     /pseudo
     CDS             2888..3874
                     /locus_tag="VGT44_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919957.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          768 a         1137 c         1151 g          818 t
ORIGIN      
        1 cttgctgagc ttgtcttcga gagaggtctg gaagcgatgc tccacacgct gttctaatcg
       61 aaccattctt gtgctgcgga gagaggcgaa aaggaaacca accggttacg actgtttcga
      121 gagccaatca gacggggcaa ccccgccgat gatgtggggg atgttgaggc cgagaaccgc
      181 aggatagccg tacagagcaa agcgtgcggt ccagagcgtc atctgttggg gagaggaggc
      241 catcccgttc tcaatccacc aagccagcat ccccaccaac cagtgcgcta gactgatgat
      301 cacgaactcc gctggcgcgg cctgcgggtc atcggctact ctcaggcctg ctgcggagag
      361 ggttcgggcg cggcttcgaa tggcttgcgc aaagtagtca cgcatctgga cggtaaacca
      421 ggaactgccg cgtttgccaa gcatcacccg atagagcctc gcgtgcctgg cgacatgctc
      481 gaagaacgct gaccaggctg ccaactggga ctcgatgtag gactgtacgg ccctggcatc
      541 aagtttgctc tcggacgaga ggagaccctc caggttcttc gggaggggac cgatctcgcg
      601 gaacatctga ttgaccgcgt cctcaaagat gcccgtgacc agggcatatt tgtctgggta
      661 gtggcgataa aacgtcgtcc ggttgaccat cgctcgctcg gcaatatctc ccacggtgac
      721 tgcatcgaac cccttttcct cgatgagatc gatcagggct tcgcgcaagt tcgcctgggt
      781 tcgtctgacg cgtaaatcaa cctggtcgcg catcagtttt gctcctgtgt tgcatatgca
      841 acattctcat gaatttgctt cttgctcttg gctgacactt tgagtacgct tcttttagac
      901 aacattctat cacaaaggta agagagtatt gcaaaacgca gaccccattg ggaggaaaga
      961 tacgatgaca aacaccccag ccattctggc agaaggactg tccaaagcgt atggcaagat
     1021 ccacgcccta gatgggttgg acctggtggc ggaagcaggg acggtcctag gcgtgctcgg
     1081 tcccaacggc gcgggcaaaa cgacgacggt gcgcatcctg acgaccctca tcaagcctga
     1141 tcgtggacgc gcggaggtgg cagggctgga cgtggtgaaa cacgccgcga ccgtgcgcga
     1201 gcgcatcggg ctcaccggtc aatatgccgc catcgatgaa ctgctcaccg gctacgaaaa
     1261 cttgaagatg ttcgggcaac tctctcgctt gccaggcgca gtggccaggc ggcgagcaca
     1321 cgacctgctt gcacgctttg atctcgaaga ggccgccgga cgcctggtca aaacgtactc
     1381 aggaggcatg cgccggcggc tcgacctggc ggccagcctg atcctttcgc ctccgatcct
     1441 gttcctggat gagcccacga ccggtctgga tccgcgtgga cgcctcgcgc tatgggaggt
     1501 gatccgtgac ctggcggccc agggcacgac ggtgttgctg accacccagt acctggagga
     1561 agctgatcac cttgcccatc agattgtggt gattgctcag gggcgcgtga ttgctcaagg
     1621 cacagcctcc gaactgaaaa cgcagatcgg cggggagcgc ctggaactca ccgtggcacc
     1681 gcacagcgat ctggacgccg cgtggcaagc gatccgtccg tacagctcag gtgagattca
     1741 gctcgaccac gaacgccgtc acctcaccgt cccggtcacg aatggagcga cgctcctcgc
     1801 cgcaatcgtg cgcgatctcg acggagcaca gattacgctt tctgatctgg ccctgcgtcg
     1861 cccaacgctc gatgatgtct ttttctcgct caccgggcac gcggtgtccg aagccgagca
     1921 gcctgaagag gcacgactac aaaggagtca acgatgaaaa accctgtcct cttaaccagg
     1981 gaagaggtga tcccgcgttc ccatttcgcg ctctattgga cgttcgccca cgcgtgggtg
     2041 ctggcgaaac gccgactcac gcagatccca cgtcgtcccg acgagttgct gatcgccacg
     2101 ctgcaaccca ccatgctggt cctgctcttc cgctacgtct ttggcggagc cattcgcgtt
     2161 gctggcacga gctatgcgaa ctatctaatg gccggggtgt tcgtgcttgc atccctggtc
     2221 ggttctctgg ccaccgggat tggcctggca actgacctcc agcagggctt gcttgaccgc
     2281 tttcgctcgt tgccgatggc gcgctcggca gtccttaccg gtcgcacgct ggccgacctc
     2341 gtccgcaatg gcttcgtcgt gctggtcatc tgggccgtcg gtctgctcgt gggcttcagg
     2401 ccagcaggtc cccccctcgc ctgggttgcg gcaacgggcg tgcttctgct gaccaccttt
     2461 acgttctcgt ggctcgcggt cctgcttggc ctggtagtca gcagtccaga agctgttcag
     2521 ggggccagtt tggtactgtt ttatcccttg ctctttggga gcaatgcgtt cgtccccacc
     2581 agcaccatgc ctgactggct gcgtgcgttc gcggaccatc agcccatttc ggtggtcatt
     2641 ggcgccgtac gtgggctgct gctcaaccag ccggatgctg cgaccatcgg gcaggcgctc
     2701 acctggtgtg tggtcctgct gatgatcttc atcgggcttt caatctgggc ctatggacgc
     2761 cgcaccgcga aatagaacgg atgagaaaag tgagaatgct atggcaagaa aaaacggtcg
     2821 ccccgctgca acgtcggaac aggtgctcac tcccttgtgt acgcactgtg ctggatgtgg
     2881 tcacccgatg cggatcgctt atcggacctg gcggaccatt acgaccttgc aggggatctg
     2941 ccgtctgacg ctccgtgttc gccgatgtcg caaccaagag tgcccacgat atcattgtgt
     3001 gtatcgaccc gaagaagagg gaagatgggc tttaccacac ggcgagtttg gtctggatgt
     3061 gattgcgctg gttggcacgc tgcgctatat cgcacatcgg agcattcctg aaatccatca
     3121 ggcgctccgt gatcgaggtg ttccgattgc cgaacggacc gtaacgcatc tgcttcagcg
     3181 ctatgaggaa ttagtcgcgt tgcatctggc agaccagcga cgattacacg agcgcttgaa
     3241 agagcaggga cagattgtgc ttgccatcga cggattacaa ccagatgttg ggcatgaggt
     3301 cctctgggtg ctgcgtgact gcctctctgg ggaagtgctc ttggctcgca gtttgctcag
     3361 cgcttgtgaa acggagctgg ccagtctgct ccgcgaggtg caacgagccc ttgctgtccc
     3421 cattcgtgga gtgatctccg acggacagca ctctcttcgc aaggctgttc aagctgtgct
     3481 gccggaggtt ccgcatcagt tgtgccactt ccattacctg cgcgaagcgg ccaagccagt
     3541 gtatgaagca gatcggcatg ccaaaaagga tttgaaaaag tatctgcgtg gggtgcgccc
     3601 aattgagcgg gccgttgaac accgtagcga tgcggaagcc gaagtgatcg gtggttattg
     3661 cttagcagtt cgcagcgctt taaccgatga tggcagacca ccgttagcgc agatgtgacg
     3721 caaggatatg actgggtgca tcgcgccgcc catatcctct ccaatgacga caagctgtcg
     3781 gcaacagagg tacgtcagac ctacgaaacg ctcttgaaag agatggagca cacgctgacg
     3841 tcctctgagc cccttgccac catgctctcg acct
//
LOCUS       DASZAK010001173         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_491727, whole genome shotgun sequence.
ACCESSION   DASZAK010001173 DASZAK010000000
VERSION     DASZAK010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491727"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            238..1887
                     /locus_tag="VGT44_20770"
     CDS             238..1887
                     /locus_tag="VGT44_20770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV2583305.1"
                     /translation="MSLTGRLLTWWMHLPPAETHDIVITHDRQIPMPDGTVLLADHYA
                     PRTSPKLPTLLIRSPYGRRGFFGGVMALPYAERGYQVLIQSCRGTAGSGGQFVYARDE
                     HEDGLTTIEWIKQQEWFSGEFATLSGSYLGFVQWAVAADAGPELKAMVPQVTTSDFNH
                     FRFQGGSFNLEAHLGWSTMMTAQAATGLQISLWSILAQGRRARKLEQAFGHLPLGEAD
                     RMVINRPSEHFQSPLLHGPDDDYWKPVDFSPRVAEVNAPAYLMGGWYDVFLDWQLKDY
                     QALRAAGKQPYLLVGPWTHTAFSADAMTVQESLVWLDTHVKGKPGRLREAPVRLYVMG
                     ENKWRDFADWPPPARSERWHLQPAGALAPAIPPASEPDHYFYDPSDPTPAVGGNSLGA
                     RKRMGPKDNRSLEARPDVLVYTSAALEQDMEIIGPITADLYVRSSLEHTDFFARLCVV
                     EESGKSLNLCDGILRLTPGSITPEPDGSLHIAIEVWPTAYHFRKGQRLRVQISSGAHP
                     RFARNLGSGEPLATGTKLCVANQTIYHDPEHPSALLLPVLL"
     gene            complement(1898..>2644)
                     /locus_tag="VGT44_20775"
     CDS             complement(1898..>2644)
                     /locus_tag="VGT44_20775"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2583306.1"
                     /translation="VAWSPDGSRLAIGYTGDKRDTETIHIILVTTGERIFTYSGGGSF
                     WGAQDDRSINALAWSPDGARIAWGGGENKVEVWDAQMRRQLAVYKGHNSAVYALAWSH
                     DGRQIASSGADDAVHIWDASTGRNAATFFKHSSVVTGVAWSSNGQRVATASHDRTALV
                     WNAVTGTTLTTHRGHTDRVLALSWSPDGSRLATASRDGQAHIWDSSRGVNTYTYTGHV
                     GSVNAISWSPDGKRIASAGADHTVHVWRAV"
BASE COUNT          503 a          818 c          789 g          534 t
ORIGIN      
        1 gtatgctctt ctttacacta cgatcccata cagcacaatc atatactgtt cacacggatt
       61 tttcagcttt tacgttgctt caaccttccc ttgcgtagac tcatctgctc atggtacagt
      121 gtgaacacat ccatttcggt ccgtaggcgc cccttttcgt cattccagag acgcttcaca
      181 gacagtccgg gacgtagtgg tgaagcgagg tacgttgcag aacacaggag gtatccgatg
      241 agtttgactg gccgtctcct cacctggtgg atgcacctgc caccggctga aacgcacgat
      301 attgtgatca cccacgaccg gcagattccc atgcctgatg gaaccgtcct gcttgcggac
      361 cattatgcgc ctcgcacgag tccaaagctc ccaacgcttc tgatccgctc cccgtatggc
      421 aggagaggct ttttcggcgg cgtgatggct cttccctatg ctgagcgcgg ctaccaggtc
      481 ctgattcaga gctgtcgcgg gaccgcaggc tcgggaggtc agttcgtcta tgctcgcgat
      541 gaacatgagg atgggctgac cacgatcgag tggattaagc agcaggagtg gttctccggc
      601 gagttcgcca cgctcagcgg cagctacctc ggctttgtgc aatgggccgt ggccgccgac
      661 gccggaccgg aactaaaagc catggtaccc caggtcacca cctctgattt caaccatttt
      721 cgcttccagg gtggatcgtt caatctggaa gcccaccttg gctggtcgac catgatgacc
      781 gcccaggccg ccacgggact acagatcagt ctctggtcga tcctcgcgca gggccgacgg
      841 gcgcgcaaac tggagcaggc ttttggacac ctgccgctcg gcgaggccga ccggatggtg
      901 atcaaccggc catctgagca tttccaatcg cccctcctgc atggacctga cgacgactac
      961 tggaaaccgg tcgatttcag ccctcgcgtc gcggaagtga acgcgcctgc gtatctcatg
     1021 ggcggctggt acgatgtttt cctggactgg cagctcaagg actaccaggc cctgcgcgct
     1081 gccgggaagc agccgtactt gcttgttggt ccctggacgc atacggcgtt ctccgccgac
     1141 gcgatgacgg tgcaggagtc gctggtctgg ctggatacac atgtcaaagg caagccaggc
     1201 aggctgcgcg aagcccccgt ccggctgtat gtcatgggcg agaacaaatg gcgcgatttc
     1261 gcggattggc ctccacccgc ccgcagcgag cgctggcacc ttcagcccgc aggcgcgctg
     1321 gcacccgcca ttccaccagc ctcggaaccg gaccattact tctatgatcc gtccgatccc
     1381 acgcctgcag tggggggaaa ttcgctcggg gccaggaaac gtatggggcc aaaggataat
     1441 cgctcgctag aagcacgccc ggatgtgcta gtgtatacca gcgccgcact ggagcaagac
     1501 atggagatca tcggcccgat cactgccgat ctctatgtcc gttccagcct ggaacacacc
     1561 gatttcttcg ccaggctatg cgtggtggag gaatcgggca aatctctcaa cctgtgcgac
     1621 ggtattctgc gcctgacgcc gggcagcatc actccagaac ccgacggcag tctgcatatt
     1681 gcgattgagg tctggccaac ggcttaccat ttccgcaaag ggcagcgtct gcgcgtccag
     1741 atctccagcg gcgcgcaccc acgcttcgcg cgcaatttag gcagcggcga accactggct
     1801 accgggacga agctctgtgt tgccaaccag acgatctatc acgatccaga acatccttcg
     1861 gctcttctgc tgccagtcct tctgtaacga gtggaggtta tacggctcgc cagacgtgga
     1921 cggtatggtc tgcgcctgcg gaagcgatgc gtttgccgtc gggcgaccag gagatggcgt
     1981 tgacgctgcc gacatggccg gtgtaagtgt aggtattgac gccccggctg gaatcccaga
     2041 tatgcgcctg gccgtcgcgg ctggcggtag cgaggcggct gccatcgggg gaccacgaga
     2101 gagcaaggac ccggtcagtg tgtccgcgat gggtggtcag ggttgtgcct gtgactgcat
     2161 tccagacgag cgcagtgcgg tcgtggctgg ccgtcgccac gcgctgcccg ttggacgacc
     2221 acgccacacc agtgacgacg ctggaatgct tgaagaaggt ggcggcattg cggcctgtcg
     2281 aggcatccca gatatgtacg gcatcgtccg cgccgctcga agcgatctgg cgaccgtcgt
     2341 gcgaccaggc cagggcatag acggcgctgt tatgtccctt gtagacggcc agttggcggc
     2401 gcatctgcgc atcccagacc tcgactttgt tttcgccccc gccccaggca atgcgcgcac
     2461 catcagggga ccaggccagc gcgttgattg atcgatcatc ttgcgcgccc cagaagcttc
     2521 cgccgccaga ataggtaaag atccgctcgc cggtcgtcac caggatgatg tgtatcgtct
     2581 ccgtgtcgcg cttatctccc gtatagccaa tggcgaggcg cgaaccatca ggcgaccagg
     2641 cgac
//
LOCUS       DASZAK010001174         1583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_102614, whole genome shotgun sequence.
ACCESSION   DASZAK010001174 DASZAK010000000
VERSION     DASZAK010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102614"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VGT44_20780"
     CDS             complement(<1..183)
                     /locus_tag="VGT44_20780"
                     /inference="COORDINATES: protein motif:HMM:NF022317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 family protein"
                     /protein_id="HEV2583307.1"
                     /translation="MSPQALATAILAVHLGVVLFNVGGLVLVPLGAWLGWRWVRNFWL
                     RVLHVLSLAVVAAQALM"
     gene            complement(451..1470)
                     /locus_tag="VGT44_20785"
     CDS             complement(451..1470)
                     /locus_tag="VGT44_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV2583308.1"
                     /translation="MNEISTVGLDIAKKVFQVHAVDGAGIVVSRRSLRRSQVLAWFAK
                     LPPCLVGLEACATAHYWARELQKLGHDVRLIPPAYAKAYVRRNKNDPADAAAICEAVG
                     RPSMRFVTIKTAQQQAMVGIHRVRDLLMKQQTMLRNQLRGLMGECGIVAATGQRGLGE
                     LLAILADPADQRIPRLLREALGVIAEMLRGFERKLAGIDRQIVQTGRGDATYRHLITA
                     PGYGPILSSAMAAMVVNPAAFRRSSDFAASLGLVPRQEGTGGKVRLGPISKRGNGYLR
                     RLLVNGAMSVLRSKRAKDDPWLAKLLASKPRKVAAVALANRMARIGWALMTRQEDFRT
                     LPATA"
BASE COUNT          294 a          531 c          468 g          290 t
ORIGIN      
        1 catgagagct tgcgccgcga cgacggccag cgacaacacg tgtagcacgc gcagccagaa
       61 gttccgcacc cagcgccagc ccagccacgc gcccagcggc acgagtacga gcccgccgac
      121 attgaataga acgacgccga gatgaaccgc gaggatcgcc gtggcgaggg cttggggcga
      181 catctctaat ctctaatccg tcatgtgtgg atggcccccc gcttgccagg gttttgatgc
      241 gcatggccgt gcggttacct gcggtcatgt gtacggcctc tgatagcggc atcgcatggc
      301 cgcgggccct gatgggtacc ggagatcgag cctgatcatg ccctcggggt cgcaatgccc
      361 catgacgaat agcaggtttg tctcgatccg tcggttgacc gacattgcca tcacgcgtgg
      421 tcaccctcgc aagtcttccg cgtctgccgg ttaagctgtc gccggcaggg ttctgaagtc
      481 ttcctggcgc gtcattagcg cccagccgat gcgtgccatt ctattggcga gcgccactgc
      541 ggccaccttg cgcggcttgg aagcgagcag ttttgccaac caggggtcgt ctttggcgcg
      601 tttgctcctc aggaccgaca tggcgccatt gaccagcagc cggcgaaggt agccgttgcc
      661 gcgcttgctg atcgggccga gcctcacctt gccgccggtc ccctcttggc gggggaccag
      721 acccagcgac gcggcgaagt ccgagctccg gcggaacgcg gccggattga cgaccatcgc
      781 cgccatcgca ctcgagagga taggcccata gccaggagcg gtgatcaggt ggcgataggt
      841 ggcatcaccg cggcccgtct gaacgatctg tcgatcgatc ccggcgagct ttcgttcgaa
      901 gccgcgaagc atctccgcga tcaccccgag cgcctcgcgt agtaaacgcg gaatgcgctg
      961 atctgccgga tccgccagga tagccagcaa ttcgccgaga ccgcgctggc cagttgccgc
     1021 gacgataccg cactctccca tcaaaccgcg gagctgattc ctgagcatcg tctgctgctt
     1081 catcagcaga tcgcgaaccc gatgaatgcc aaccatcgct tgctgctgcg cggtcttgat
     1141 cgtgacgaag cgcatcgagg gacgacccac cgcctcgcag atcgccgccg cgtccgccgg
     1201 atcgttcttg ttccgacgca cgtaagcctt cgcataggcc ggtggaatca atcgtacatc
     1261 atgaccaagc ttctgtagct cgcgagccca atagtgcgcc gtcgcgcatg cctccaggcc
     1321 caccaggcac ggcggcagct ttgcaaacca tgccaatacc tgagagcgcc gcagcgaacg
     1381 ccgcgacacc acgatgcccg caccgtcaac cgcatgcacc tggaagacct tcttggcgat
     1441 gtccagaccc accgtgctaa tctcgttcat ggatggctcc cgtttaagtt ggttcctaac
     1501 agtccaactt tggcacatcg atgccgtaaa gggggccatc cacccatcat gcccggactt
     1561 gttccgggca tccacgtcgg gcc
//
LOCUS       DASZAK010001175         5808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_421259, whole genome shotgun sequence.
ACCESSION   DASZAK010001175 DASZAK010000000
VERSION     DASZAK010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421259"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..734
                     /locus_tag="VGT44_20790"
     CDS             429..734
                     /locus_tag="VGT44_20790"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HEV2583309.1"
                     /translation="MNFDDKTLRCRECGSDFVFTAGEQEFYQQKGLMNQPSRCPACRT
                     ARRASGGVGNRAERAPREMHTVICAECGVETQVPFLPKNDRPVYCSSCYDKVRVARG"
     gene            1067..3343
                     /locus_tag="VGT44_20795"
     CDS             1067..3343
                     /locus_tag="VGT44_20795"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV2583310.1"
                     /translation="MTLEDLLAETRQYLAPPDLERIQRAFHVADVAHGGVLRRSGEPY
                     IEHPLEVALLLADMRIDADSIVAALLHDVVEDTDFPLDELRAQFGAAVAGIVDGVTKF
                     DFFAKKESGNAFEPPSRETPEQLEQRQHLTRDLKRRQRSETVRKMLLAMAEDPRVVVL
                     KLADRLHNMRTLGVMDHSQQQNKARETREIYSPLARRLGMSLVQAELEDLAFSYLEPD
                     KFTQLAHEVEEERRKRQPQIDEICRMLREEMEKAGIHAEVRFWQKHLSSINRKLEQSG
                     TELSQIHDLVSYRILVDTVHDCYLALGHIHALWRPKDGRIKDFIATPKVNGYQSLHTT
                     VFCLDNQLAEIQIRTHEMERIADYGLASYWYLRERGGARLSTREMVTWIEQLREWQRE
                     LPENADEFVEAVKGDIFQEQIFVFTPKGEVKDLPRGSTPLDLAYRIHSDIGDHCAGAR
                     IITNLEESGRLITRIVPLDYELKGGEIVDVMVSAEAHPTREWLSFARTTSARTKIRRY
                     LKTYEHEVNLQLGRERLDIALKALGAPGMRVISDDDLLGMHVSKRYGSSDDVLVALGR
                     GDLQVGAVLECLTPLLQERGDLRHETDGAKNAKNGHASDGAGENELPQAVVKLAHCCC
                     PLPGDAIVGLLNPNKGIIVHASDCRTLRRFRGQEADRLLEMNWLQIDPQRYLAPVVIV
                     AHDRSGLLRDVAAVVADAGINMTAVSSNTNTSLQKATITATLEIDRVDQIEPIFERMR
                     QVKNVVSVSRDRSRRIQG"
     gene            3436..>5808
                     /locus_tag="VGT44_20800"
     CDS             3436..>5808
                     /locus_tag="VGT44_20800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEV2583311.1"
                     /translation="MSEPALAQQLFDLVRRSMPPDDVAAIEQAFALAQAACGERRGEK
                     QLLLIEHALAISVILAEMHIDAIGVASGLVFEAVDADLLPLERVEQALGVPVARVVGS
                     MLRLNILERKKQSMAQEYKAANGESEATREQKKLRIREALRRQQAETVRKMFFAMSED
                     PRVVLLKLAYRLHAMRLIVAKRYPVDTQESQVMAQESREIYAPLAGRLGMSRVESELD
                     DLAFEVLEPDKYAWVREQVEVESTHWRDYVERVCGVLRDEMQKLGLHAEVNGRVKHFY
                     SFYKKARRNAGDVERLEDLKMAVDINQIHDLIAFRILVDTTPDCYIALCHVHSLWKPK
                     DGRIKDFIANPKPNGYQALHTTVFCLENQLVEIQIRTFAMHELAEYGVAMHWHYKDVG
                     DHATASARELLSWLGRLADWQRELRAATTTDSEFVEAVRDEIFDEQIFVFTPKGEVKD
                     LPVGSTPLDFAYRVHSKIGDRCAGARVTSQSNDGERLVTRLVPLDYELRSGDIVDIVT
                     NRTAHPTRDWLALARTAAARSKIRRYLKTHERDINIQIGRERLDRELKTMGLRGIEAV
                     NEDAQSWLPGEFQKGSFDDLLVAVGGDEIRQHAVAVKLMDYLREREQKEGKDQKEDDD
                     ALALPLASKGSSSARLQVAGVSGLMTSLANCCCPLPGDEIVGFISRGKGAVIHRADCR
                     NIERYRERDRERLVNVNWTSMGQQRYLAPIAIMARDRSGLIRDIASVVSEVGVNMTSV
                     ASSVSAGKETVMINVTLEIDGQEQLQRVFTRLERVKGLMGVERDLGKVKKS"
BASE COUNT         1267 a         1489 c         1846 g         1206 t
ORIGIN      
        1 catgttggct tcggtcctaa actttcatca ttgcgctcct acggatcttt ctcactcgac
       61 tgtcactata cataacgtat gatgtggtac agagtagcca tttctgtatt cagtaccggg
      121 tgtcatcggt acgcttgaag gatatgaatc cgcgtgtatg gatgagctta tccctttttg
      181 acgttactta gtgtacaaaa tacacataaa aaatgtataa agatgcaggt atttagataa
      241 attgcggagg caaaaggctt gcaaattggc ggaaaagcgt gtatgcttgt gggcggtatc
      301 cgatctcggt ctggcatcag tgctcttcca gaataatagg acccgcgctg ctaggggtaa
      361 cacaaactgg tgactctgca aggatatgga cccggaagaa taccatcata tcacaaggag
      421 gtctctagtt gaacttcgac gacaaaaccc ttaggtgtcg ggaatgcgga agcgatttcg
      481 tattcactgc gggtgaacaa gagttttacc agcaaaaagg attgatgaac cagcccagcc
      541 gctgtcctgc gtgccgcacg gcgcgccgcg catccggtgg tgtgggcaat cgcgcggagc
      601 gtgccccacg cgaaatgcac acggtgatct gcgccgagtg cggcgtggag acgcaggtgc
      661 cgttcttgcc aaagaacgac cgccccgtct attgtagttc atgctacgat aaggtgcggg
      721 tcgcgcgcgg ctaagctata cctgcgcaag cgggcgtagt ggaagccacc acatgaatca
      781 tttgggataa ggccaaggta tgttcgcggc cttgtctctt tttttgtttg ctttgcatgc
      841 acacgtcttt acattggggt gggtgctgtt acgttcggca tagattgttc gtaccaaatg
      901 ttatatttta tagggctaca tatgcgttgc attgtctatg cctgtaaact acactatgtt
      961 atagtatgat taaacaaaag cgagtagaag cagtctggct tcccggattg cccagggtgg
     1021 ccctatgtta gttgtgaatc cgattgaatc agtggcgatc tctcatgtga cacttgaaga
     1081 tctgctggcg gagacgcgcc agtatctagc tccacctgac ctggagcgga tacaacgtgc
     1141 ttttcacgtt gcggatgtag cacacggggg agttttgcgt cgttcgggcg agccatatat
     1201 tgagcatccc ctggaggtag cgctgctgct ggccgatatg cgtatcgatg ccgatagcat
     1261 cgtggccgcg ttgctgcatg acgtggttga agacaccgac tttccgctgg atgaattgcg
     1321 cgcgcagttt ggggctgccg ttgccggcat cgttgatggc gtgaccaagt tcgacttttt
     1381 tgcgaaaaag gagagcggca atgcgtttga gccgccatcg cgcgagacgc cggagcagct
     1441 tgagcagcga caacacctga cgcgcgattt gaagaggcgg cagaggtcgg aaacagtgcg
     1501 gaagatgcta ctggcgatgg cggaagaccc gcgcgtggta gtacttaaac tggccgaccg
     1561 gctgcataat atgcgcacat taggcgtgat ggatcactcg cagcagcaga ataaggcgcg
     1621 cgaaacaagg gagatttact ctccgctggc gcggcgactg ggcatgtcgt tggtgcaggc
     1681 ggaactggaa gacctggcgt tcagctacct ggaaccggac aaattcacgc agctggcgca
     1741 cgaggtggag gaggagcggc gcaaacggca gccgcaaatc gatgagattt gcaggatgct
     1801 gcgcgaagag atggagaagg cgggcattca tgctgaggta cgtttctggc agaagcacct
     1861 gtcgagcatc aaccgcaaac tggagcagag cgggacagaa ctcagccaga ttcacgacct
     1921 ggtctcctat cgtattctgg tcgatacagt gcatgactgc tacctggcgc tggggcatat
     1981 tcacgcgctg tggcggccca aagatgggcg gatcaaggac ttcatcgcca cgcccaaggt
     2041 gaacgggtac caatcgctgc acacgaccgt cttctgcctg gacaatcaac tggcggagat
     2101 acagattcgc acgcacgaga tggagcgcat cgccgattat ggactcgcca gttactggta
     2161 cctgcgcgag cgcggcggag cgaggttatc gacgcgcgag atggtgacat ggatcgagca
     2221 actgcgcgag tggcagcgcg agttgccgga gaatgccgac gagtttgtcg aagccgtcaa
     2281 aggagacatc ttccaggagc agattttcgt tttcacgccc aagggcgagg tgaaggacct
     2341 gccgcgtggc tcgacgccgc ttgacctggc ctatcgtatt cactcggata ttggcgatca
     2401 ctgcgccggg gcgcgcatca tcaccaacct ggaggagagc gggcggctga taacgcgcat
     2461 cgtcccgctg gactacgaac tcaagggcgg cgaaattgtg gatgtgatgg tcagcgcgga
     2521 ggcgcatccc acgcgcgagt ggctctcgtt tgcgcggacc acatcggcgc gcacgaagat
     2581 tcgacgctac ttgaaaacct atgaacacga agtgaacctg cagctgggac gcgagcggtt
     2641 ggatatcgcg ttgaaggccc tgggcgcgcc ggggatgcgg gtcatttccg acgatgattt
     2701 gctggggatg catgtgagca agcgctatgg ctcgtccgat gatgtgctgg tggctttagg
     2761 gcgaggggat ctccaggttg gcgcggtgct ggaatgcctg acaccgctcc tgcaagagcg
     2821 tggtgacttg aggcacgaga ccgatggcgc taaaaatgcg aagaatggac atgccagcga
     2881 tggcgcgggc gagaatgagc tgccccaggc ggtggtcaag ctggcgcact gctgctgtcc
     2941 attgccgggc gatgcaatcg tcgggttact caatccaaat aaagggatca ttgtgcatgc
     3001 cagcgattgc cgtacactgc ggcgctttcg tggccaggag gctgatcgct tgctagagat
     3061 gaactggctg cagattgatc cgcagcgtta cctggcgcct gtggtcatcg tggcgcatga
     3121 ccggtccggg ctgttgcgtg atgtggcggc ggtggtggcc gacgctggca tcaatatgac
     3181 ggcggtctcg tccaatacca acacctcgct gcagaaggcc accatcaccg cgacgctgga
     3241 aatcgatcgc gttgaccaga tcgagccgat ttttgagcgg atgcggcagg tgaagaatgt
     3301 ggtcagtgtg agccgggacc gcagccgccg tatccagggc tgatatgttt caattgatga
     3361 attgtactgg aaacgtccat gatagatata cctgtaagtc aacctgatca acctgtagtc
     3421 agtcaatcca cgactatgtc tgagccggca ctggcccaac agttgttcga cctcgtgcgg
     3481 cgctcgatgc ctcccgacga tgtagccgcc atagagcagg ccttcgcgct ggcccaggcg
     3541 gcctgtggag agagacgtgg cgaaaaacaa ctgctgctga tcgaacatgc cttagcgatc
     3601 tctgtgatcc tggcggagat gcatattgat gccatcggcg tcgcctccgg cctggtcttc
     3661 gaggcggtcg atgctgatct gctgccgctg gagcgggtcg aacaggcgtt gggtgtgccg
     3721 gtggcgcgtg tcgtgggcag tatgctgcgc ctgaatatcc tggagcgcaa aaaacagagc
     3781 atggcgcagg aatataaggc ggccaacggt gagagtgagg ccacgcgcga gcagaagaag
     3841 ctgcgtatcc gtgaagcgct gcgccgccag caagccgaga cggtacgcaa gatgttcttc
     3901 gccatgtcgg aggacccgcg cgttgtgttg ctcaagctgg cctatcggct gcatgccatg
     3961 cgcctgatcg tggcgaagag gtatcccgtc gatacgcagg agtcgcaggt gatggcgcag
     4021 gagtcgcgcg agatctacgc gccgctggcc gggcggctgg gtatgtcgcg cgtcgaaagt
     4081 gagctggacg acctggcctt cgaggtgttg gaaccggata aatatgcctg ggtgcgcgaa
     4141 caggttgagg tcgaaagcac acattggcgc gattatgttg agcgcgtctg cggcgttttg
     4201 cgggatgaaa tgcagaaact tgggctgcac gctgaggtca acgggcgcgt caagcatttc
     4261 tacagttttt ataagaaggc gcggcgcaac gctggagatg ttgagcggct ggaagatctc
     4321 aaaatggcgg tggatatcaa ccagattcat gatttgatcg cgttccgcat tctggttgat
     4381 acgacgcccg attgctatat cgcactttgc cacgttcaca gcctgtggaa accgaaggat
     4441 gggcgcatca aagacttcat cgccaatccc aagccgaatg gctaccaggc gctgcatacg
     4501 actgtcttct gcctggagaa ccagctcgtc gagatacaga ttcgcacgtt tgccatgcac
     4561 gaactggcgg aatatggtgt ggcgatgcac tggcattaca aggatgtggg cgatcatgct
     4621 acagcttcgg ccagagagct gctttcgtgg cttggtaggc tggccgattg gcagcgcgag
     4681 ttgcgcgcgg ccaccacgac cgacagcgag tttgtcgaag cggtgcgcga cgaaattttc
     4741 gacgagcaga ttttcgtctt cacgcccaaa ggcgaggtca aagatttgcc agttggttcg
     4801 acgccgcttg attttgccta tcgcgtccac tcgaagatcg gcgatcgctg cgccggagcg
     4861 cgtgtgacat cgcagagcaa cgatggcgaa cgactggtga cacgcctcgt gccgctcgat
     4921 tatgaactgc gcagcggcga tatcgtggat atagtgacca atcgcaccgc gcatcccacg
     4981 cgcgattggt tagctctcgc gcgcactgcc gccgcgcgca gcaagattcg ccgctacctc
     5041 aaaacgcacg aacgcgatat caatatccag atcgggcgcg aacgactgga ccgcgaactc
     5101 aagacgatgg ggttgcgcgg catcgaggcg gtgaacgaag acgcgcagag ctggctgccg
     5161 ggcgaattcc agaagggcag cttcgatgac ttgctggtag cggtcggcgg cgacgagatt
     5221 cgccagcacg ccgtcgccgt caagttgatg gattacttgc gcgagcgcga acaaaaagag
     5281 ggcaaggatc agaaagaaga cgatgacgcg ctggcgctgc ctctcgccag caaaggctct
     5341 tcctcggctc gcctgcaagt cgccggtgtg agtggcctga tgaccagcct ggcgaactgc
     5401 tgttgcccgc tgcccggcga tgagatcgtc ggcttcatca gccggggcaa aggcgcggtc
     5461 attcaccgtg ccgattgccg caatatcgag cggtatcgcg aacgtgaccg ggagcggctg
     5521 gtgaacgtca actggaccag catgggccag cagcgttacc ttgcccccat cgcaatcatg
     5581 gcgcgcgatc gttccggcct gatccgcgac atcgccagcg tcgtctcgga ggtcggtgtc
     5641 aacatgacct cggtggcttc cagtgtgagc gcgggcaagg agacggtaat gatcaatgtc
     5701 acactggaga tcgatggaca ggagcagctt cagcgcgtct tcacacgact ggaacgggtg
     5761 aagggattga tgggagtcga gagggatctg gggaaagtca agaagagc
//
LOCUS       DASZAK010001176         2394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_102662, whole genome shotgun sequence.
ACCESSION   DASZAK010001176 DASZAK010000000
VERSION     DASZAK010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102662"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="VGT44_20805"
     CDS             <1..419
                     /locus_tag="VGT44_20805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583312.1"
                     /translation="PPVFLARRPAEPLDSDLQAFYLKLLATVKASGLREGEWHLCERS
                     GWPDNSSYLNLVAWCWSQGEAPYLVVVNLSEYRSQAQVHLPWADLAGSTWQLADALNN
                     TLFERDGDDMQTTGLYVDLPAWRFHFLRFQSGQNAG"
     gene            complement(427..1287)
                     /locus_tag="VGT44_20810"
     CDS             complement(427..1287)
                     /locus_tag="VGT44_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583313.1"
                     /translation="MAETYVERVHTENAVLDIGEDIGALVIYTGQELLGEEIEVSPKG
                     TNAQKTHTAVLERKVNGRTMYAALFLALPEGAYNTCSIPSSAITITGGHVAELDWRES
                     NVIIEPKTPYGSHPHRHEYAVLEMLPPRYRNGKVISAAPMGSAPMRYTEDGQVAWDEM
                     WTDFCDLALAGGPPHRDTLLEPVPPDEVKVDPENYERVVSEIERGWRLVTRLPTVRSE
                     KSGWVGLQCEDEEMALWILRAIVVENVCVRREGSVLFLPAGPAFRLDKEIKNVITVVA
                     KTHHYWTEHL"
     gene            complement(1375..>2394)
                     /locus_tag="VGT44_20815"
     CDS             complement(1375..>2394)
                     /locus_tag="VGT44_20815"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEV2583314.1"
                     /translation="STLLASNLPCPPCNIGPRSTPNSQQLAGAAVQKLPGGGRVFAGQ
                     RAEGFYVDLGSVFDLADLRPFQNLHLIPSPAQPGVNGTQGYNVHSIALQLPKKLLTAD
                     GSTPTDPADPRSVIGVYTSASRQRSTIIQGNGQFTESGAFVKVSRLGEPLINEVIIPL
                     GQKDFWNSQDPQGDSQFANFYAHPELAALLPVLYPGVFPNLAAYSKARADLEAILLTG
                     IPAGIIPGFQNFTGTTQADFLRLNMSIAPTAQPNILGILGGDLAGFPNGRRVFDDIVA
                     VELRAVAGVTIPLVDSSFKPDAAASQVNDFSPPAIPSGVRYQQQFPYLGLPFSGYASR
                     PHPGA"
BASE COUNT          501 a          676 c          698 g          519 t
ORIGIN      
        1 ggccgccggt cttcctggca cgccgccccg ccgaaccact tgattcagac ctgcaagcgt
       61 tctatctcaa actactggcg acggtcaaag catcgggact gcgcgaaggt gaatggcatc
      121 tgtgcgagcg ctctggttgg cccgataaca gcagctactt gaacctcgta gcctggtgct
      181 ggagccaggg ggaggcgcct tacctggtcg tcgtgaacct ctcggagtac cggtcgcaag
      241 cgcaggttca cctgccctgg gccgacctgg ccggaagcac gtggcaactg gcagacgcgc
      301 tcaacaacac gctattcgag cgcgatggcg acgatatgca aacgacgggc ctgtatgttg
      361 atttgcccgc ctggcgcttt cacttcctgc gcttccaatc gggccagaac gccgggtgaa
      421 aaagttttat agatgttccg tccaatagtg atgcgtctta gcaaccaccg tgatgacgtt
      481 cttgatctcc ttatcgaggc ggaaggctgg cccggcgggc agaaagagca cgctgccctc
      541 gcgccggacg catacatttt ccacaacaat ggctcgcaat atccagagcg ccatttcctc
      601 gtcttcacat tgcaagccaa cccaccctga tttctcactt cgtacagttg gcagtctggt
      661 gaccaggcgc cagccacgtt cgatctcgga gaccacgcgt tcgtagtttt ccgggtctac
      721 tttgacctca tctggaggta caggttccaa taacgtgtct cgatgcggag gacccccggc
      781 cagcgccagg tcgcagaagt ctgtccacat ctcatcccag gctacctgcc cgtcttctgt
      841 gtaacgcatt ggagcagagc ccattggcgc tgcgctgata acctttccat tgcgatagcg
      901 cggcggaagc atctctaata cggcgtactc gtggcgatgt ggatgactgc catagggagt
      961 ctttggttca atgatgacgt tggattcccg ccagtccagc tcagcgacgt gaccaccagt
     1021 gatcgtaatc gcactggagg gaatactgca ggtgttgtaa gccccttcag gcagcgcgag
     1081 aaagagagca gcgtacatgg tacgcccatt gaccttacgt tccaggacgg cggtatgtgt
     1141 cttctgggca ttggtgcctt tcgggcttac ctcgatctcc tcaccaagca gctcctgccc
     1201 ggtatagatc accagcgccc cgatatcctc cccgatatca agcacagcat tctccgtatg
     1261 aaccctctca acatacgtct cagccatcag ctctctcctc tcggttgaac agaagtggag
     1321 cccggcagtt caaaaccacc gagctcctct aatcgcgctc gggtagagca gcttttatgc
     1381 tccaggatgc ggtcggcttg catagccact aaacggcagt ccgagatacg ggaactgctg
     1441 ttgataacgt accccgcttg gaatagccgg aggactgaaa tcgttgacct gggatgccgc
     1501 tgcatcaggt ttgaaactcg aatcgaccag ggggatggtg acgccagcaa ccgcgcgcag
     1561 ctcgaccgct acgatgtcat cgaagacgcg ccggccgttc gggaatccgg ccaggtcgcc
     1621 acccaggatt ccaagtatgt tcggttgtgc tgttggggca atggacatat tcagtcgcag
     1681 gaaatccgcc tgtgtagtac cggtgaaatt ctggaagccg ggaatgatac ccgctggtat
     1741 cccggtgagt aagatggctt ccagatccgc tcgcgctttg ctgtaggccg cgagattggg
     1801 gaacactcct ggatagagga caggcagcag ggcagctaac tctggatgag cataaaagtt
     1861 ggcaaattga ctatcgccct ggggatcttg cgagttccaa aagtccttct ggccaagggg
     1921 gatgatgact tcgttgatga gtggttcgcc gagtcgcgag accttcacga atgctccgga
     1981 ctctgtgaac tgaccattgc cttgaataat tgttgatctc tgacggctcg ccgaggtata
     2041 cacgccgatc accgatcgag gatcggctgg atcagtcggc gttgagccat ccgccgtcag
     2101 gagcttcttg ggcaactgga gcgcgatgga gtgaacgtta tacccttgag ttccattcac
     2161 gcctggttga gcaggactgg gaatgaggtg caggttttgg aaaggtcgga ggtcggcaag
     2221 gtcgaagacc gagccaaggt ccacgtagaa tccctcggcg cgctgtccgg cgaaaactct
     2281 cccgccaccc ggaagctttt ggacggcggc cccggctagt tgctgggagt tgggtgtgga
     2341 tcgagggcca atgttgcagg gaggacaggg tagattgctc gccaggagcg ttga
//
LOCUS       DASZAK010001177         1543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_421281, whole genome shotgun sequence.
ACCESSION   DASZAK010001177 DASZAK010000000
VERSION     DASZAK010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421281"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VGT44_20820"
     CDS             <1..228
                     /locus_tag="VGT44_20820"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HEV2583315.1"
                     /translation="YAYDIEEAVEELCCVAAPIRDHSGEVVAAMSLSVPTYRFKQYTN
                     QYRLAIVGAARQVSENLGYIAVKRCSSSGSR"
     gene            306..1307
                     /gene="argF"
                     /locus_tag="VGT44_20825"
     CDS             306..1307
                     /gene="argF"
                     /locus_tag="VGT44_20825"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV2583316.1"
                     /translation="MSLSLSGRSFLKLDDFTPQEITFLLQLSAELKADRYAGTEQQRL
                     KGKRIALIFEKDSTRTRSGFEVAAYDQGAHVTYLGPGGSHIGSKESMKDTARVLGRLY
                     DGIEYRGFYQADAEELARYAGVPVWNGLTNEAHPTQVLADLLTMQEHSRKPLEEVALC
                     FLGDAGHNMADSLMIGAAKMGMDMRLAGPGTTWPTEERLSVARAFTAETGGRITLTED
                     VEAAVNGCDFLYTDVWVSMGEPADLWAERITEMLPYQVNGRLMAMTHNSATKFMHCLP
                     ALHNTETKLGKEIYENYGLDALEVTDDVFESQASIVFDQAENRLHTIKAIMVATLTS"
BASE COUNT          351 a          430 c          446 g          316 t
ORIGIN      
        1 tacgcctatg acatagaaga agccgttgag gaactctgct gcgtagccgc gccgatccgc
       61 gatcacagcg gcgaagtcgt ggcggccatg agcctgtccg tgcctaccta tcgcttcaaa
      121 caatatacga atcaatatcg cctcgccatc gtcggtgcgg ccaggcaggt ttcagagaat
      181 ctcggctata tcgctgtaaa acgctgttcc tcgagtggat cgcgttaatt cctcctcttc
      241 gcgtgatacc gaatgccggt tatataatgg ggtcatgcac atcttgtata cgaggaggga
      301 ataggatgag cctcagcttg agtggtagaa gtttcttgaa gctggacgat ttcacgccgc
      361 aggaaatcac gttcttgctg caattatcag ccgaactgaa agcggacagg tatgcaggaa
      421 ccgagcagca gcgtctgaaa gggaaacgga tcgcgcttat ttttgaaaag gactcgacgc
      481 gcacccgcag cggctttgaa gtcgccgcgt atgaccaggg ggcgcatgtg acgtacctcg
      541 gcccaggtgg ctcgcacatc ggctcgaagg aatcgatgaa agacaccgcc cgcgtgcttg
      601 gccgcctgta cgacgggatc gagtatcgcg gcttctacca ggccgatgcg gaagaacttg
      661 cgcgctacgc tggcgtcccc gtgtggaacg gtctgaccaa cgaggcgcac ccgacgcagg
      721 tgctggccga tttgctcacg atgcaggagc atagccgcaa gccgctagaa gaggttgcgc
      781 tctgctttct gggcgatgcc ggtcacaata tggcggattc cctgatgatc ggggcagcca
      841 agatgggcat ggatatgcgc ctggcgggtc ccggcacaac ctggcctacg gaggaacggc
      901 tgagtgtggc ccgtgccttc actgccgaga cgggcggacg cattactctg acggaggacg
      961 ttgaggctgc cgtcaacggc tgtgacttcc tctacaccga tgtgtgggtc tcgatgggcg
     1021 aacctgccga cctgtgggcc gagcgcatta cagagatgct gccctaccag gtgaatggcc
     1081 ggctgatggc catgacacac aattctgcca cgaaattcat gcactgcctg cctgccctcc
     1141 acaacactga gacgaaactt ggcaaagaga tctacgaaaa ctatggactg gacgccctcg
     1201 aagtgaccga tgacgtgttc gagtcgcagg cgtcgatcgt ctttgaccag gccgagaacc
     1261 gcctgcacac catcaaggcc attatggtcg cgaccctcac ctcctgaggt gcgcttgaaa
     1321 aagagcgcct gcttgaccgt gtataccttg tgaatccacc gatgtttttg tacaacgaga
     1381 gaagtgaaga atggatcata tcgatactct tgacacggcc ccgattctaa cgcccaggca
     1441 ttacaacgct ccctcgatat ttaccccaca aaacttgttg cgggaagccc ggcggcaaaa
     1501 aaggttgcca ccaggagcag ttccagacat ctgtgtgctt gat
//
LOCUS       DASZAK010001178         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_349441, whole genome shotgun sequence.
ACCESSION   DASZAK010001178 DASZAK010000000
VERSION     DASZAK010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349441"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..774)
                     /gene="tatC"
                     /locus_tag="VGT44_20830"
     CDS             complement(<1..774)
                     /gene="tatC"
                     /locus_tag="VGT44_20830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEV2583317.1"
                     /translation="MATSNTDQRNTMSAVDDDNILDEDAELDGATMSLVDHLEELRWR
                     IFKSLIAVVVGAIVTFIFRVQLMNFLTLPLPKTADQIGNGRLVATGLAEGFTVFLKLS
                     VAGGILLAIPVLLYQVWAFISPGLYAHEKKKALPFVFVGIFLFLAGIALGFIVLQYPV
                     NWLVSFASTSFTELVTADSYFTFVAFFLLAFGIVFEIPLVLTFMAQLGLITSQTLRRK
                     RPTAHVGMWIAATFLTPGADFYSPIILGIAMSCLFELTII"
     gene            complement(953..1126)
                     /locus_tag="VGT44_20835"
                     /pseudo
     CDS             complement(953..1126)
                     /locus_tag="VGT44_20835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            complement(1268..1564)
                     /locus_tag="VGT44_20840"
     CDS             complement(1268..1564)
                     /locus_tag="VGT44_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583318.1"
                     /translation="MQNDSIETLVLRHYGNAAPTPDGLEATLLASVRHEARELERRTS
                     RATAWRERPISRRRVLRLVAFGTAGLSVVGVGLENLRRVEASLLGQDTNKPAYS"
     gene            complement(1582..>2111)
                     /locus_tag="VGT44_20845"
     CDS             complement(1582..>2111)
                     /locus_tag="VGT44_20845"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEV2583319.1"
                     /translation="IAVNIVRMQQRRSRLFRIIPFARLEKDQAERSTTRTNSSFDVID
                     QQAIPNQPPGYNAPQQPDLEDMVAERDAIQRTLQRISAPLRECLMLSSIGQFSSAEIA
                     AMLDIDEAAVRQRIARARKQFQQIYTQLGGEELADAPSPPTSSTPATSMSQNRLDMHH
                     SQREHFERHFSHFLS"
BASE COUNT          447 a          537 c          651 g          476 t
ORIGIN      
        1 gatgatggtc agctcgaaga ggcaggacat ggctatgccg agaataatcg ggctgtagaa
       61 gtccgcgccg ggcgtgagga aggttgccgc gatccacatg ccgacgtgcg ccgttgggcg
      121 tttgcgtcgt aatgtctgcg aggtgataag accgagctgc gccatgaagg tcaacacgag
      181 cgggatctca aagacgatgc cgaaggccag taggaagaag gctacaaagg taaagtagct
      241 atcggctgtg accagttcgg tgaagctcgt cgatgcgaag ctgaccagcc agttgaccgg
      301 gtattgcagc acgataaacc ccagtgcgat gcccgccaga aagaggaaga tgccaacaaa
      361 gacgaatggt aaggcttttt tcttctcgtg cgcgtacaga cctggtgaaa tgaaagccca
      421 gacctggtat aacagcactg gaatggcgag caggataccc ccggcaaccg agagcttgag
      481 gaagacagtg aagccctccg cgagtccggt ggcaacaagc ctgccgtttc caatttgatc
      541 ggcagttttg ggcagcggca acgtcaggaa attcatgagt tgtacgcgga agatgaaggt
      601 cacaattgcg ccgacgacaa cagcgatcaa cgatttgaag atgcgccagc gcagttcttc
      661 cagatggtcc accagcgaca tggtggctcc atccagttca gcatcctcgt cgagtatgtt
      721 gtcgtcatca acagccgaca tggtattgcg ctgatcagtg ttactggttg ccatatttgc
      781 tcctgtagag cgggttgcgc gtgtttacga ggtccccgct gggtggcaac cactcttact
      841 caaccggcgt ttccgcgcgt gggacctcgt tgctgacggc cttcgcgctg gtttcgccct
      901 cataagcgac gctgccgagg cccagtttgc tcatatcgcg cgaactgacg ggcatcttcg
      961 gctcttcttc ttctttcggc gcggtcaatt cattcatgcc cttgcggaac tctttgatgc
     1021 ttttgccgat ggccgacccc atctccggca gctttttggg gccgaagatc aagagggcga
     1081 tgatcagaat gatgaccaga tcgaaaccgt gaaatcctgg aatcatggga agtgtatcct
     1141 ttcatatcaa gcgacaatca aacgctggtt gtttgctttc tatcctgtac tacgttgaac
     1201 tcccaccgcc ggatgttcga tactccttgt ggccgataaa tcggcaccta cgggaggtcg
     1261 ggtttcatca tgaataggcg ggtttgtttg tatcctggcc gagcaaagaa gcttctactc
     1321 tacgcagatt ctctaaaccg acgccgacga cgctcaagcc ggctgtgccg aatgccacca
     1381 gtcgtaacac gcgtcgtcgg ctgatgggtc gttcgcgcca ggcggtggca cgcgaagtgc
     1441 gccgctccag ctcccgtgcc tcgtggcgaa cggaagcgag cagcgttgcc tcaaggccgt
     1501 cgggtgtggg agccgcgttc ccgtagtggc gcagcaccag tgtctcgatc gagtcgttct
     1561 gcatgacttc tccgtgagcg gtcaggatag aaaatgagag aaatgacgct caaaatgttc
     1621 tctttgacta tgatgcatgt cgagacgatt ttgtgacatg gatgtcgcgg gggttgagga
     1681 tgtgggaggt gatggcgcat cggcaagctc ttcgccgccc aactgcgtgt agatttgctg
     1741 gaattgtttg cgcgcacggg cgatgcgctg gcgcactgcc gcttcgtcga tgtccagcat
     1801 ggcggcgatt tctgccgagg agaactggcc gatactggag agcatcagac attcacgtag
     1861 aggggcgctg atgcgttgta gagtgcgttg gatggcatcg cgttcagcta ccatatcttc
     1921 gaggtcgggc tgctggggcg cgttgtagcc aggtggctgg tttggaatgg cctgttggtc
     1981 gatgacgtcg aaagaggaat tggtccgcgt ggtcgagcgt tcggcctggt ctttttccag
     2041 gcgagcgaaa gggatgatac ggaagaggcg actgcgacgc tgctgcatac gcacgatatt
     2101 gaccgcgatt t
//
LOCUS       DASZAK010001179         1813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_65022, whole genome shotgun sequence.
ACCESSION   DASZAK010001179 DASZAK010000000
VERSION     DASZAK010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65022"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..973
                     /locus_tag="VGT44_20850"
     CDS             <1..973
                     /locus_tag="VGT44_20850"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2583320.1"
                     /translation="FFMAIGAYTAAYLGGNVPSSPLDPLGLALPFYIWLPAAGIVAAL
                     VGALIGPTALRLKGFYLGIVSLALVFIGQYLFLNARGITGGPQGRTFPVPAIGTASID
                     QQNSFFGIALTSGQQYFLLILIVLALAAFFVYNVMRSRAGRAFRAVRDNETGATIMGV
                     NLFEAKMGAFILSSFLAGIAGALFASYSRYIIPDYWSLTLSIQFVAAIIIGGIASVWG
                     SILGAAFVFGLPLVIDQFSLLPASGSGIITSGDLNAILYGLLIIVFLLFEPGGVIGLA
                     RRVQALTRKFSSSKDEGGEPAEITDLPSSTGSQPDSGNSTLTKGESA"
     gene            1083..>1813
                     /locus_tag="VGT44_20855"
     CDS             1083..>1813
                     /locus_tag="VGT44_20855"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2583321.1"
                     /translation="MLTKKKLFACLGIIIVGVMALSGCASNSSSGTSGSVLKTGPGVD
                     VAHKTITLGILSPSSGPVAAPIGDPLALGVKVFFDHVNDNGGINGYKVNFLEEDTQYN
                     PQIEVQQYQKIHNQVLMIADSLGTPTTFAIKPLADADHMLVYAASLSSALAREKYLIL
                     AGTPYRLQVENAFDYVVNKLGVKNPATGIIYQNDEYGQDGLTGYKEAVSGYQLHDVAQ
                     ASYAPTDTDFTAQVSQMKAAGAKYV"
BASE COUNT          372 a          505 c          517 g          419 t
ORIGIN      
        1 gttcttcatg gcgattggcg cgtacactgc cgcttatctc ggtggcaatg tgccgtcatc
       61 tccgctcgat ccgctcggcc tggccttgcc tttctacatc tggcttccgg cggcaggcat
      121 tgtcgccgcg ctggtgggag cgctgatcgg tccgacagcg ctgcggctca aagggttcta
      181 cctgggcatt gtatcgctgg ccctggtctt catcgggcaa tacctctttc tgaacgcgcg
      241 cggtataaca ggcggcccgc agggaaggac gttccccgtc ccagccattg gaactgcttc
      301 aattgaccag caaaactcct tctttggtat tgcgctcacg tcgggccagc aatatttctt
      361 gttgattctc atcgtattag cactggcggc attctttgtg tataacgtca tgcgttcgcg
      421 ggccgggcgc gcgttccgag cggtgcggga caacgaaacg ggcgcgacca tcatgggtgt
      481 caacctgttt gaagcgaaga tgggcgcgtt catcctttcc tcgtttctgg ccggcattgc
      541 aggcgctctg tttgcctcgt atagccgtta tattatccct gactactgga gtttgacgct
      601 ctctatccag tttgtcgcgg caatcatcat cggcggcatc gccagcgtct ggggatcaat
      661 cctgggagca gcatttgtgt tcggattgcc gctggtcatt gaccaattct cactgctgcc
      721 tgcaagcggt tcgggaatca tcactagtgg cgacctgaac gcgattcttt atggcctgct
      781 gattatcgtc ttcctgctct ttgagccggg aggcgtgatt ggacttgcgc gccgggtcca
      841 ggcgttgacc cgcaagttca gttcatctaa agacgaagga ggtgaaccag ccgagataac
      901 cgatctgccg tcgagcacgg gatcccagcc tgacagcggg aactcgacgc tcacaaaagg
      961 cgaaagcgca tagtcttttg cgcgcggcgg gataggtaaa acagagcagg attcaggaga
     1021 tgtagcgcat tttacatgct caatcgtcgt tattttgaat cttcgatgaa gaggtgcaac
     1081 acatgcttac caagaaaaag ctctttgcgt gcttgggtat cattatcgtc ggcgtgatgg
     1141 cgctctccgg atgtgccagc aacagctcaa gcggcaccag tgggagcgta ctcaaaactg
     1201 gtcccggcgt cgatgtagcg cataagacca tcaccctggg aatactctcg ccttcctccg
     1261 gtccggtggc agccccgatt ggggatccgc tggcgctagg cgtgaaggtg ttctttgacc
     1321 acgtcaatga taatggcggg atcaacggct acaaggtaaa cttcctggag gaagatacgc
     1381 agtacaatcc acaaattgag gtgcagcaat accaaaagat tcataaccag gtgctgatga
     1441 tcgccgatag cctgggcacg ccgacgacgt tcgcgatcaa acccctggct gacgctgatc
     1501 acatgctggt gtatgcggcc tcgctctcct cagccctggc gcgggagaag tacctcatcc
     1561 ttgcgggcac accctaccgc ctgcaggtcg agaatgcctt cgattacgtc gtcaacaagc
     1621 tcggcgtcaa gaatcccgcg acgggcatta tctaccagaa tgacgagtac ggccaggatg
     1681 gactgaccgg ctacaaagag gctgttagcg gctaccaatt gcacgatgtg gcccaggcca
     1741 gttacgcgcc gaccgataca gactttaccg cgcaggtctc gcagatgaaa gcagcggggg
     1801 cgaagtacgt ctt
//
LOCUS       DASZAK010001180         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_670522, whole genome shotgun sequence.
ACCESSION   DASZAK010001180 DASZAK010000000
VERSION     DASZAK010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670522"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..512)
                     /locus_tag="VGT44_20860"
     CDS             complement(150..512)
                     /locus_tag="VGT44_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEV2583322.1"
                     /translation="MTTNVGYLYAAIMLAAGFAFVILTMTVSNLIAPHKRTKEKLQTY
                     ESGETPIGTARVQYPLGFYIFALLFVAFDVDIVFIISWAVIFKQLSFFGFFEILIFII
                     ILALGLAYAWRKGVIRWI"
     gene            complement(749..>2028)
                     /locus_tag="VGT44_20865"
     CDS             complement(749..>2028)
                     /locus_tag="VGT44_20865"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEV2583323.1"
                     /translation="VLLLVLVSLVFGRRRALRKKNQEEMDRFKEEAAAARRNAPLAAR
                     RIEEETQRRRRQPDDAGAPYGQQPSALQAPPAPVPSVPLVLPTAPLPQPGQQSPPDYA
                     FPPSDELDTPTMPISRKNGYGDTDRQKEGHVVRELHNVSLAVGSLSNVGIKRQHKPNE
                     DSLFAMQGARTHNSQPQQFGLFVIADGMGGHANGGDASRTAIQNMIDFMLPKISVADP
                     TMDDQGYLKLLEEGVQRANLAVHQRNLQDRADMGTTMTTALVIGAMAYVANVGDSRTY
                     LYRQSTGLTKVTRDHSVVASLVDAGIIKPDDIYTHPKRNQIYRSLGEKPVVEVDSFTV
                     ELQAGDKLLLCSDGLWDLVRDPDIQRIIASTDSNPTRTTEELIKAALDGGGDDNVSVI
                     VVSITDIDGHTGLTGIHILSKPDTITVPELPTM"
BASE COUNT          413 a          542 c          628 g          445 t
ORIGIN      
        1 gacctcgatg cccagataat cgccttgcaa tgtgcgagcc ccaacttcct tgccggtaat
       61 ctgcgcgatg ggcgccaggt cacgggcgag cgcagcattc tgatcccgat agccggtagt
      121 tgcgctgggc aggctccttg cgcgcggtac taaatccatc gtataactcc cttgcgccag
      181 gcataggcca ggccaagcgc gagaatgatg atgaagatca ggatttcaaa gaatccgaag
      241 aagctcaact gtttgaaaat tacggcccag gagatgataa acacgatatc gacatcgaag
      301 gcgacgaaga gcaaagcgaa gatatagaag ccgagtggat actgcaccct ggctgtgccg
      361 atgggcgtct cgcccgattc gtaggtttgc agtttttcct tcgtgcgttt gtgcggggca
      421 atcaagtttg acactgtcat ggtgagtatg acaaaggcga agcccgccgc gagcatgatg
      481 gctgcatata aataaccaac gttggtagtc acgtgccttc ctccaaattg cccacgcgct
      541 gatcatttgc cgcgcgtaga ggccctaggt gctcttattc cgaccggcaa cgacgggctt
      601 attccgctct gcaacgacgc tgcgcgtcta cactgcgtgt cagcagaggg gaaaggagta
      661 ctgcgtcctg gctgcgccca gccggtcgga gcggtagaat gctgttttga ttatagcata
      721 ggggtgaagg ggcgacaaca aaacatcatc acatggtcgg caattccggc acggttatgg
      781 tgtccggctt ggacaggata tggataccgg tcaggccggt atgcccatcg atatctgtga
      841 tggagacgac gatgacactg acattatcgt cgccgccgcc atcgagggcg gccttgatca
      901 attcctcggt ggtcctggtg ggattggaat ccgttgaggc gatgatacgc tggatatcgg
      961 ggtcgcgtac cagatcccac aggccatctg agcagagtaa gagcttatcg ccggcctgca
     1021 gctccaccgt gaacgaatcg acctcgacga ccggcttttc gcccaggctg cgatagatct
     1081 gatttcgttt gggatgggta tagatatcgt ctggtttgat gatgccggca tcaaccaggc
     1141 tggcgaccac ggagtgatcg cgcgtgacct tcgtgagtcc cgttgattgc cggtaaagat
     1201 aggttcgact atcgccaaca ttggcgacgt aggccatcgc gccaatcacc agggcggttg
     1261 tcatggttgt tcccatgtcg gcgcgatcct gcaaattgcg ctggtgtacg gccaggttgg
     1321 cgcgctgtac gccttcttcg agcaatttga gatagccctg gtcgtccatg gtgggatcgg
     1381 caaccgagat tttcggcagc atgaaatcga tcatgttctg gatggcggtg cggctcgcgt
     1441 cgcctccgtt ggcgtggccg cccatgccat cggcgatgac gaagaggccg aattgctgtg
     1501 gctgcgaatt atgggtcctg gctccctgca tggcgaacag gctatcctcg ttcggcttat
     1561 gctggcgttt aatcccaaca ttggagagcg atccgactgc cagactcaca ttatgcaatt
     1621 cgcgcacaac gtggccttct ttttgacggt cggtatcgcc gtatccattc ttccggctga
     1681 tcggcatggt tggcgtgtct agctcatccg agggagggaa agcataatcc ggtggggatt
     1741 gctggccagg ctgaggtagg ggagcagtag gaagcaccag gggcacggaa ggaacagggg
     1801 cagggggcgc ctggagtgcc gacggctgct gaccgtatgg cgcgcccgcg tcgtctggct
     1861 ggcgacgacg acgctgcgtt tcctcttcga tgcggcgcgc agccagtggg gcattgcggc
     1921 gtgccgcggc ggcctcttcc ttgaagcgat ccatctcttc ctgatttttt ttacgcagcg
     1981 cgcgccgtcg cccaaagacc aggctgacca ggacgaggag cagcacaa
//
LOCUS       DASZAK010001181         4507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_599034, whole genome shotgun sequence.
ACCESSION   DASZAK010001181 DASZAK010000000
VERSION     DASZAK010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599034"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VGT44_20870"
     CDS             <1..388
                     /locus_tag="VGT44_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583324.1"
                     /translation="AFTIVLASISGWMLGMRNAMITTLTEDYMLLAEAKGLSERRIML
                     AYAARNAILPNVTGFALSLGFVVGGALLTEAVFSYPGIGFALLQAAQNSDYPLLQGIF
                     LLIAMAVLGANFIADLVYVILDPRVR"
     gene            520..1440
                     /locus_tag="VGT44_20875"
     CDS             520..1440
                     /locus_tag="VGT44_20875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583325.1"
                     /translation="MQWQHSSPLASLLRSLWRIFTLNRKATIGFCIVLFFVLVALIGP
                     LFIHTDPSAFSNDVLVPPSAAHWLGTTQTGQDIFSQLVVGTRVSILWSFTTGLVVTAF
                     SVIVGLTAGYLGGIVDDLLNLFINVFLVLPGFPLAVVLAAYLPLKGPLTVALVITLTS
                     WAYQARVLRAQTLSMRHRDFVDASRSNGESTRRIIFFEVFPNEIAIVAAGFVGTAIYV
                     ILAAAGLEFLGLGDITVIDWGTMFYWAQNNDALLLGAWWWYVAPGMSIALLGAGLALI
                     NFGIDEVANPRLRVETRLFKRIWLFHVFRK"
     gene            1510..2910
                     /locus_tag="VGT44_20880"
     CDS             1510..2910
                     /locus_tag="VGT44_20880"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEV2583326.1"
                     /translation="MSIIPNTLSQTQVETDLAERFPTHFLWGAATASYQIEGAIHEDG
                     RGASIWDDFAATPGKTYQGQSGEIAADHYHRVEEDTNLMVELGLSAYRFSIAWPRILP
                     EGRGAINPAGLDFYDRLVDTLLAKGIQPFATLYHWDLPSSLEQAGGWLNRATAYAFAD
                     YAELVTRRLGDRVTSWITLNEPWCSAYLGYGVGAHAPGLRNRQAAIDAAHHLLLGHGL
                     AVSRMRAYLAADAQVGITLDLQQIYGADERAETARDMELAKAFNNGWFLDPIYHGAYP
                     ERFFEHLALNPPPIQEGDLAIIAAPLDFLGVNTYSRILIRGAHTQPLADRFEAVSPVP
                     NACYTDMGWEIYPHGLRDLLVEVGRNYPVGNLYVTENGAAFPDVWDGGNTVSDPQRVD
                     YLRSHITACAEAIEQGAPLRGYFVWSLMDNFEWAEGYTKRFGIIYIDYSTQRRVIKES
                     GRWYATLLRAFRNKDR"
     gene            3312..4280
                     /locus_tag="VGT44_20885"
     CDS             3312..4280
                     /locus_tag="VGT44_20885"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2583327.1"
                     /translation="MSEQQIQKVRVKRLAHVGLWATDVAAQTRFYRQALGFHLRATEI
                     NSTDQDVEFEDANTFLSLGDEHHCLGLFNDTRTATSNGRSPVSHTRLHHTAYEVDTDA
                     ELAALAARLNQSGIKLALEPRDGNPQAGDTLWFNDPDGNRIEISVTPDDSLTLPPASS
                     EGRRTRLHPHALQHLAICTSRMEAMVDFYTEALGFDISDWLLRECVWLRCSNDHHTLI
                     IMRGKPDIDHIGYSVPTGPELLAWADYLSRQQTPILWGPGRHGAGNDLFLRFADAENV
                     HIELSAELQQYYDHDVTTPPRLWHSRPTALNLWGVMPAWVREEAHV"
     gene            complement(4315..>4507)
                     /locus_tag="VGT44_20890"
     CDS             complement(4315..>4507)
                     /locus_tag="VGT44_20890"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2583328.1"
                     /translation="TEDEVYYIISGRGFIRVGAEEHPVEAGTLVYVKAHVEHRFHTIT
                     EDLRVLVFFAPAEYSAAQS"
BASE COUNT          936 a         1236 c         1228 g         1107 t
ORIGIN      
        1 ggcctttacg attgttctgg cctcgatctc cggctggatg ttggggatgc gcaacgcgat
       61 gattactacc ctgaccgagg actatatgct gctagccgag gccaagggac tgagcgagcg
      121 ccggatcatg ctcgcctatg cagcccgcaa tgctattctg cccaatgtga ccggctttgc
      181 cctttctctg ggctttgtcg tcggcggcgc gctgttgact gaggcggtct tctcctatcc
      241 cggcattggt tttgctctgc tgcaggcggc gcaaaactca gactatccac tgctgcaggg
      301 aattttcctg ctgatcgcca tggccgtgct gggtgccaat tttattgctg acctggttta
      361 tgtgattctt gatccgcgtg tacgctaaag gaaggagcga ttgagatgag tctatttcca
      421 caatcgcgcg ggagcgaatc acaacctgcg gtttctccct cgcttgtaga gagcgttgtt
      481 ccctcctcag taagatggtc tgagaagggg aacgcatccg tgcagtggca gcactcgtcg
      541 ccgctggcca gtcttttgcg ttccctgtgg cggattttta ccctgaatcg caaagcgact
      601 attgggttct gtattgttct cttttttgtt ctggtggcgc ttatcggccc gctatttatc
      661 cataccgatc ccagcgcgtt ctcaaatgat gtgcttgttc caccttcagc ggctcactgg
      721 ctgggtacaa ctcagacagg acaggatatc tttagccagc ttgttgtcgg aacacgcgtc
      781 tcgattctct ggagctttac gactggtctg gtggtgacag cgttctctgt aatcgtgggt
      841 ctaacggcgg gctatcttgg tgggattgtc gatgatctgc tcaacttatt catcaatgtc
      901 tttctggtcc tgccgggatt ccccctggcg gtggttctgg cggcttatct gccgctgaaa
      961 ggcccgctca cagtggccct ggtgatcaca ctgacaagtt gggcctatca ggcacgtgtg
     1021 ctgcgcgccc agacgctttc gatgcgccac cgcgactttg ttgatgcctc gcgctccaac
     1081 ggtgagtcga cccggcggat tatcttcttt gaggtctttc ccaatgaaat cgcgattgtg
     1141 gctgcgggtt ttgtgggcac ggcgatctat gtcattctgg ccgcagccgg cctggagttt
     1201 ctcggtctgg gagacataac cgtgatcgac tggggcacga tgttctactg ggcacagaat
     1261 aacgacgcac tcttactggg agcctggtgg tggtatgtgg cgcctggcat gtctattgcc
     1321 ctgttgggtg ctggcctggc tctgatcaac tttggcatcg atgaggtggc caatccgcgc
     1381 ttgcgcgtag agaccaggct gttcaagcgt atctggctct tccacgtttt tcgtaaatga
     1441 gagacacagg ccagctaaaa cataaatact tttttaaatg gtatcacttc acaacgaata
     1501 ggagaacaca tgagcattat tcccaacaca cttagccaga ctcaggttga gactgatctg
     1561 gcagagagat tccccacgca tttcctctgg ggcgcggcca ctgcctctta tcagatcgag
     1621 ggtgcaatcc acgaggatgg ccggggtgcc agcatatggg acgatttcgc ggccacgcca
     1681 ggcaagacct atcaaggcca gagcggggag atagcagccg accattatca ccgggttgaa
     1741 gaagatacca acctgatggt agagcttggc ctgagcgcat atcgtttctc gattgcctgg
     1801 ccgcgcatcc tacccgaggg acgtggcgcg atcaacccgg ccggcctgga tttctatgat
     1861 cgcttggtgg acacgctgtt ggcgaaaggc attcagccct ttgcaacact ctatcactgg
     1921 gatcttccct cgtcgctgga gcaggccggt ggctggctca atcgcgccac cgcctatgcc
     1981 ttcgccgatt atgccgagct cgtgacccgg cgccttggag accgcgtcac gagctggatt
     2041 acgctcaacg agccctggtg tagcgcgtat ctgggctacg gggtcggtgc gcatgcaccc
     2101 ggcctgcgta atcgccaggc ggcgatcgac gctgctcatc atctcctgtt gggacacggc
     2161 ctggccgtat cacgaatgcg tgcttatctg gcggccgacg cacaggtggg aattactctt
     2221 gaccttcagc agatctatgg tgccgacgaa cgggccgaga cggcgcgcga tatggaactg
     2281 gccaaagcct ttaataacgg ctggtttctt gatccgatct atcatggtgc ctatcccgaa
     2341 cggttcttcg agcacctggc actcaatccg ccaccaattc aggagggtga cctggcgatt
     2401 atcgcagctc ccctggattt tctgggtgtg aatacctact cgcgcatcct gattcgcggc
     2461 gcgcacacgc agccgctggc cgatcgcttt gaagctgtct cgcccgtgcc caatgcctgc
     2521 tataccgata tgggctggga gatctatccg cacggtctgc gcgatctgct tgtcgaagtg
     2581 gggcgcaact atccggtggg caatctctat gtgaccgaga acggcgcggc cttccccgat
     2641 gtctgggacg gcggcaacac ggtcagcgat ccccagcgcg tcgactatct acgctcgcat
     2701 atcacagcct gcgccgaggc catcgagcaa ggagctccac tgcgcggcta ctttgtctgg
     2761 tcgctgatgg acaattttga gtgggccgag ggctatacca agcgctttgg gataatctat
     2821 atcgactatt ctacccagcg cagggtgatc aaagagagtg gccgctggta tgccacgctg
     2881 ctgcgcgcct ttcgcaacaa ggatagatga aaacaattct taaaaaagtc agagatcatg
     2941 tgcagacgtc aagcttgcac atgatctgtt gtcatttgtc gaatatcatg cttgccctac
     3001 cacatcgata ccaaaatccc acacacaccc gcctgcagac gccgttcatg gcaatcgaca
     3061 gatataaggg ggatgtggag attctggctt gattctcgtg tgtcgtccgt tgacgcgtgc
     3121 tattgctcca ttagggtcac tctaggacgg atgacagcaa tgcggcacgg atacagacgg
     3181 acaaaagagc atcaccgcga agcgccatgt gaccttcggg taatttcgtt gaccctcacg
     3241 gtacgcctgt gtatgataca cgttaataca tgtgaataga tagcacatgc attgtgtacc
     3301 aggaggtcat tatgagcgag cagcaaatac agaaggttcg agtcaagcgg ttggcccatg
     3361 tcgggctatg ggcaacggat gtggcagccc agacacgatt ttaccgccag gcgctaggat
     3421 ttcatctgcg ggctaccgaa atcaattcaa cagaccagga tgtcgaattt gaagatgcaa
     3481 acacctttct ttctcttggg gatgagcacc attgcctggg actatttaat gatacaagaa
     3541 ctgctaccag taatggacgc agtcctgtgt cgcacactcg cctccatcat accgcctacg
     3601 aagttgacac cgatgctgaa ctcgctgctt tagctgctcg tctcaaccaa tctggtatca
     3661 agttggcatt agagccgcga gacggaaatc cacaggcagg tgacacactc tggttcaatg
     3721 atcctgatgg gaaccgcatc gaaatatcag ttacaccaga cgattcgctg acactacctc
     3781 ccgcgagtag tgaaggacga cggacacgtc tccacccaca tgcattgcaa cacctggcga
     3841 tctgcacctc gcgtatggag gccatggtcg atttttatac cgaggcgcta ggtttcgata
     3901 tttccgactg gctgttgcgt gaatgtgtct ggctgcgctg cagcaatgat catcataccc
     3961 tcattatcat gagagggaaa ccagatatcg atcatattgg ctatagcgtc cctaccggcc
     4021 ccgaattact tgcctgggcc gattatctga gtcgtcaaca gacaccaatc ctgtggggac
     4081 ctggtcgcca tggcgctggg aacgatctat ttttgcgttt tgccgatgca gaaaatgtgc
     4141 atatcgaact ctcggcagag cttcaacaat actatgatca cgatgtgacg acaccgccac
     4201 gcctatggca ctcgcgtccc accgcgctca acctctgggg agttatgcca gcctgggttc
     4261 gtgaagaagc acacgtgtga gatatccgat cttacctgaa atcgtcactt ctgtttatga
     4321 ttgcgctgcg ctatactctg ctggcgcaaa gaagaccagc actctcaagt cctccgtgat
     4381 cgtgtgaaag cgatgttcaa catgtgcttt tacgtagacc agcgttcctg cttcgactgg
     4441 atgttcctcc gcgcctactc gaatgaaccc tcgcccgctg atgatgtagt agacttcatc
     4501 ttcggta
//
LOCUS       DASZAK010001182         1910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_65100, whole genome shotgun sequence.
ACCESSION   DASZAK010001182 DASZAK010000000
VERSION     DASZAK010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65100"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VGT44_20895"
     CDS             complement(<1..1141)
                     /locus_tag="VGT44_20895"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV2583329.1"
                     /translation="MKFATNAIHAGQEPDPTTGAIMTPIYQTATYAQAGLGEHKGYEY
                     ARTGNPTRTALEACIAALEEGRYGLAFASGLAAESAILSLLNAGDHLISCDDTYGGTY
                     RIFERVLRRYNVAASYVPASDVAAYERAIQPNTRMIWLESPTNPLLRLVDIEAIASLA
                     HQHNLLVVVDNTFASPYFQQPLKLGADIVVHSTTKYLNGHSDVIGGALVTSNEEVYNS
                     IKFYQNAAGGVPGPFDAWLTLRGIKTLAVRMKQHEANALAVAKFLLEHPLVEKVYYPG
                     LPSDPDHALAMQQMSGFGGMVSFQFKGTYDDVARVVRRFRIFTLAESLGGIESLVCHP
                     ASMTHGSIPRELREARGLTDTLLRLSVGIEDIDDLLGDLEQTLNIP"
     gene            1305..1823
                     /locus_tag="VGT44_20900"
     CDS             1305..1823
                     /locus_tag="VGT44_20900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583330.1"
                     /translation="MSHPLSSKYVVRLGVVACVLLFVLAACGAGENGPVANNTNGSSG
                     TSSSQKTPTAPSAGLAQFVVTRVDMSVSPSSIDGTHCGANATITYTATFHVVANSPGG
                     TVQFGFTINNGRGESMTSLTFAPGQTAMTYTFTWSGALPGDHTYPGLGGVDVTSPNQL
                     LSELVMSAGQCS"
BASE COUNT          379 a          516 c          600 g          415 t
ORIGIN      
        1 acggaatatt taacgtttgc tctaaatctc cgagcaaatc gtcgatgtct tcgatgccga
       61 ccgagaggcg caggagggta tcggtcaggc cgcgcgcctc gcgcagttct ctgggaatgg
      121 agccgtgcgt catggaggcc gggtggcaga cgagcgactc gatgccgccg aggctttcgg
      181 ccagggtgaa gattcgaaaa cggcgcacca cgcgcgccac gtcgtcgtag gttcctttga
      241 actggaatga gaccatgccg ccgaagccgc tcatctgctg cattgccagg gcgtggtcgg
      301 gatcagaggg caggccgggg tagtagacct tttcgacaag aggatgttcg agcaggaact
      361 tcgcgacggc cagcgcgttg gcttcgtgct gcttcatgcg cacggccagt gtcttgatgc
      421 cgcgcagggt cagccaggca tcgaaaggac ccggcacacc accggcggcg ttctggtaga
      481 atttgatcga attgtagact tcttcgttgc tcgtgaccag cgccccgccg atgacatcgc
      541 tgtggccgtt caggtatttg gtcgtgctgt gtaccacgat atccgcgccc agcttcaacg
      601 gctgctggaa ataggggctg gcgaaggtat tatcgacgac gaccagcagg ttgtgctgat
      661 gcgccaggga tgcgatagcc tcgatatcta ccagccgcag cagcgggttt gtgggcgatt
      721 ccagccagat catccgagtg ttgggctgga tggctcgctc gtatgcggct acgtcgctgg
      781 cggggacata acttgctgcg acgttgtagc ggcgcaacac gcgctcgaaa atgcggtagg
      841 tgccgccata ggtgtcatcg cacgaaatga ggtggtcgcc cgcgttaagc aggctgagga
      901 tggcgctttc tgccgccagg ccggaggcga aggccagccc gtagcgtccc tcttccagcg
      961 ccgcgataca ggcttccagc gcggtacggg ttggattgcc ggtacgcgcg tattcgtagc
     1021 ctttatgttc acccagcccg gcctgcgcgt aggtcgcggt ctggtaaatc ggcgtcatga
     1081 tcgccccggt ggtcgggtca ggctcctggc cggcatggat ggcgttggtc gcaaatttca
     1141 tggtatcgct ccttcattcg gatagttcgc ttgcaagcga ctatagcact tttgtggcga
     1201 aagggcaatg aaaaatgaac tacttcacag cgcggcgggt ttattcaggg taacatacct
     1261 ggataagtgc agttggtact catattaccg aagggagaga aattatgtca catccattgt
     1321 cttcaaagta tgtcgtacga ttgggggttg ttgcttgcgt attgctcttc gttctggctg
     1381 catgtggcgc cggtgagaac ggccctgtcg ccaataatac gaatgggagc agcgggactt
     1441 catcctcgca gaaaactccc acggctccat ctgccgggct ggcgcagttc gtagtcacca
     1501 gagtcgatat gtcggtcagc ccatcctcta tcgatggcac acactgcggc gcaaacgcga
     1561 ccattacgta caccgctacc ttccacgtgg tagcgaacag ccctggtggc acggtgcagt
     1621 ttggctttac catcaataac gggcgcggcg agtcgatgac gagcctgacc tttgcgcccg
     1681 gtcaaaccgc gatgacctat acgtttacct ggagcggggc gctgcctggc gaccatacct
     1741 atcccggact gggtggggta gatgtgacca gcccgaatca gttgttgtcg gagctggtga
     1801 tgtcggcggg acaatgcagt tgaccgagac gtaacctccg cccttgtcga aacgtagtaa
     1861 tctgtgaggt tgttacatct caacgcagct acacgacaag gaaagaaggg
//
LOCUS       DASZAK010001183         2572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_491913, whole genome shotgun sequence.
ACCESSION   DASZAK010001183 DASZAK010000000
VERSION     DASZAK010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491913"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VGT44_20905"
     CDS             complement(<1..683)
                     /locus_tag="VGT44_20905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEV2583331.1"
                     /translation="MFEIIAEPNRRAILSLLVSSQQSVGEIARQLRMPQSTVSKHLRV
                     LREAGFVESTVDAQRRLYRLKPEPLQEVDAWLAPFRRFWSAHVDALERHLDHMDQSTP
                     TKRKTRRNEMKIKLTAVYVDDQDKALRFYTEVLGFAKKADVRQGPFRWLTVASPEEPD
                     GTELQLALNDNPAAKAYQQAIFQQGQPAAMFFTDDVKGDYERIKARGAEFTMPPTDVT
                     ASTIAMLNDT"
     gene            complement(794..1660)
                     /locus_tag="VGT44_20910"
     CDS             complement(794..1660)
                     /locus_tag="VGT44_20910"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2583332.1"
                     /translation="MQNEPKKEYPSTYYVQGKQSEQELERLTIQGQMMTEAMGGPLSE
                     QAEPSSLRRVLDIACGPGEWVIATAKTYPTMSLTGIDISRNMIQYAREQAGAEHLEDR
                     VSFHVMDALLLLEFPASFFDLVNLRCCVGFMRTWDWPKMIKEMRRVSRAGAVVRITDA
                     EAGHTCPASPALTQFSEWFQNALFQAGNLFEPAPSGLTGHLASLLGQHGCKAVQARTY
                     SMTYQAGTPSAKVYYDDMQRAFQTLRPFILKWGPRTAPDDYDALCQQALAEIQQSNFQ
                     ATWNLLTAWGTS"
     gene            complement(1877..2434)
                     /locus_tag="VGT44_20915"
     CDS             complement(1877..2434)
                     /locus_tag="VGT44_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2583333.1"
                     /translation="MDPLWKTALWRQFGAAIDMLENALVACPAPLWTQRLWSAPSDHS
                     LPPEFAEFWYITYHTLFWLDLYLSGSREEDFAPPAPFIWTELDPAVSPEQPYTKDELR
                     AYLATTRRKCHATLVALTDERARQPVEYPWAEGQYVSFLELQMYAMRHVQEHAAQLSL
                     FLGQHAIPDAALDRVARAKGEPGSQ"
BASE COUNT          501 a          727 c          798 g          546 t
ORIGIN      
        1 gtgtcgttca gcatcgcaat ggtcgaggcg gtcacgtcgg tgggcggcat cgtgaactcg
       61 gcgccgcggg ccttgatccg ctcgtagtcg cccttgacgt catcggtgaa gaacatggcc
      121 gcgggctggc cctgctgaaa gatagcctgc tgatacgctt tggccgcggg gttgtcgttc
      181 agcgccagct gcagctcagt gccgtccggc tcctcaggcg aggccacggt cagccagcga
      241 aatgggccct gcctgacatc ggccttcttg gcaaagccca gtacctccgt atagaagcgc
      301 agggccttgt cctggtcgtc cacgtatacc gcggtcagtt tgattttcat ttcgtttctc
      361 cttgtcttcc ttttcgttgg tgttgattga tccatatggt cgaggtggcg ttcgagagca
      421 tctacgtgag ctgaccagaa ccgacggaac ggagccagcc aggcatccac ctcctgaagt
      481 ggttcaggtt tcagccggta gagacggcgc tgtgcgtcca ccgtggattc cacgaaaccg
      541 gcctcgcgca gcacgcgcag gtgctttgac acggtcgact gcggcatacg aagttgacgc
      601 gcgatctctc caaccgactg ttgtgacgag accaggaggc tcaatatcgc gcggcggttc
      661 ggctccgcaa tgatttcgaa cacaggttcc attctgttag tatactccaa agagcatatg
      721 cgtgtcaagg tatgtacagg cagtggggcc aaggcaacgg tttcagtcgg agtgcgggga
      781 tgcacttgat gcgtcaggac gtaccccagg ccgtcagcaa gttccaggtg gcctgaaaat
      841 tactctgctg tatctcagca agggcttgct gacacagggc atcatagtca tcaggtgcgg
      901 tgcgggggcc ccatttcagg atgaagggac gcagtgtctg gaaggcacgc tgcatatcat
      961 cgtagtagac cttcgcagat ggagtgccgg cctgataggt catggaatag gttcttgcct
     1021 gcaccgcctt gcatccatgt tgcccgagga gcgaggcgag gtgaccggtc agacctgagg
     1081 gtgctggctc aaagaggttg ccggcctgga acagggcgtt ctgaaaccat tcggagaact
     1141 gcgtgagtgc gggactggca gggcaggtat ggcctgcctc tgcatccgtg atgcgcacga
     1201 cggctccggc acggctgacg cgccgcatct ctttgatcat cttcggccag tcccaggtgc
     1261 gcatgaaacc gacgcaacaa cgcaggttga ccaggtcaaa gaaggacgcg ggaaattcga
     1321 ggaggagtaa ggcgtccatg acgtgaaacg agacgcgatc ttcgagatgc tcggcgccag
     1381 cctgttcgcg tgcgtactgg atcatattcc ggctgatgtc gatgccggtg agcgacatcg
     1441 tcggataggt cttcgccgta gcaatgaccc actcgcccgg tccacaggcg atatcgagga
     1501 cacgccgcaa gctactcggc tcggcttgct ccgacagcgg accacccatc gcttccgtca
     1561 tcatctgacc ctgaatcgtt aatcgctcga gttcttgctc gctctgctta ccctgcacgt
     1621 agtaggtgct ggggtactct ttctttggct cgttctgcat cccgtagtcc tctcttctgc
     1681 attcgcgagt ggcctgattc cgcgcctttc ttcccgtccc agaccagaga atccacctgg
     1741 ccgtcaagct caattatacc ggaagcgggg aagtatcgca acagcagata ctgtccttct
     1801 ggtcgaagtc gggcagacgc tttcgggtgt atactgtttg aacgcatcga gagcgggtaa
     1861 cccactccgc aaacgtttat tggctgccag gttcaccctt cgctcgcgcg acccggtcga
     1921 gcgcggcgtc cgggatggcg tgctgtccga gaaagaggct cagttgggcc gcgtgttcct
     1981 gcacgtggcg catggcgtac atctgtaact ccaggaagct gacgtattgc ccctccgccc
     2041 aggggtattc gacgggctgg cgcgcccgct cgtccgtcag cgcgaccagc gtggcgtggc
     2101 acttgcggcg cgtagtcgcg agataggcgc gcaactcatc cttcgtatag ggctgttcgg
     2161 gcgacacggc ggggtctagc tcagtccaga taaagggagc cgggggagca aaatcctctt
     2221 cccgggagcc ggagagatac aagtcgagcc agaagagggt atgatacgtg atataccaga
     2281 actccgcgaa ctccggtggc agggagtggt ccgagggtgc gctccagagg cgctgggtcc
     2341 acagcggggc gggacaggcc accagggcgt tttcaagcat atcgatggcg gcgccgaact
     2401 gccgccagag ggcggtcttc cacaagggat ccatctgttt acctccaagt gtaagaatga
     2461 ttgacagctt gtcactccag ctgattcacg cgcgtcgtag taaattgggc caaacttcga
     2521 ccgattttac agaaacggcc cacagagtct tgggatgaca ggtgtgttca ag
//
LOCUS       DASZAK010001184         3478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_386698, whole genome shotgun sequence.
ACCESSION   DASZAK010001184 DASZAK010000000
VERSION     DASZAK010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3478
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386698"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VGT44_20920"
     CDS             complement(<1..415)
                     /locus_tag="VGT44_20920"
                     /inference="COORDINATES: protein motif:HMM:NF018822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LppX_LprAFG lipoprotein"
                     /protein_id="HEV2583334.1"
                     /translation="MGSIQRSFGRSLALVALCMASALLLSSCRLPWQHTPAAPVSNLG
                     AKPTAQQVISTMQKNFRAVTAFHVNMKVDNLGTASDGDIQIRSADGDVLMPDKVKAQA
                     NVLLSGQAVTINLVSIGATQYITDPITGQWRVVTGV"
     gene            complement(668..3085)
                     /locus_tag="VGT44_20925"
     CDS             complement(668..3085)
                     /locus_tag="VGT44_20925"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEV2583335.1"
                     /translation="MSTLEQQDLLAGLNEPQRQAVTTTQGPLLILAGPGSGKTRVITH
                     RIAYIVQHEQVSPWRILAVTFTNKAAKEMRERLEKLVGVNESKEMMLGTFHAICARVL
                     RIEADYLAPLGLNRSFVILDTGDQTTLIKQAVRDLNLDEKQYKPANMQAMISRAKNDM
                     QNPDQMAEKAAKYTEEVAARVYKKYQKLMRDNNSVDFDDLLLLTEQLWRREPEVLRRY
                     QRRWEYIHVDEFQDCNLPQYKLIRQLGFGTDDRHEGLGNVCVVGDDDQMIYTWRGASV
                     ENVIRFERDFPHTQVILLEQNYRSTQTILDAAQNVVQRNRLRKDKRLWTALGQGEKVI
                     MHEAYDEESEAEYVGIESQRLLLRGEVERRGDIAVMYRTNAQSRALEEQFMRLNIPYK
                     VIGSRKFYERKEIKDMVAYLRLLLNPYDDLSLRRIINVPNRKIGPKTLGELQQWATAE
                     HSTPYEAIQRIADHPTLGKSAKQALATFGALMKDFQQAMEELQLPELIDRIAERSGLG
                     PELREQADGDERWSNVLELRRVADDYAEIETRTALELFLENVALVGGADTTQTGEDGK
                     LITEENRDAVTLITLHAAKGLEYPVVFIIGMDEGSLPHARSVDKPEQLEEERRLAYVG
                     FTRAMRRLYLLRARRRSYFGDVVPTQPSRFLDDIPTKLVTQSGGAGSFSSSTARSNTA
                     PARRSSWDDYNQDSYAARETGRVFGSGKPPTSSSPPARPSTSSPLPAPARPSPVPPPL
                     PTSSPAPRPQSQQFKPGDRVRHATFGEGIILKSEMEQQTEFVEVQFHGKHGKKRLSLD
                     FARLEKL"
BASE COUNT          639 a          949 c         1112 g          778 t
ORIGIN      
        1 gaacacccgt cacgacgcgc cactggccgg tgatggggtc ggtgatatac tgcgtagcgc
       61 cgatgctgac gaggttgatg gtgacggcct ggccggagag cagcacgttg gcctgcgcct
      121 tgaccttatc gggcatcagg acatcgccgt ccgcgctgcg aatctggata tccccatcgg
      181 atgccgttcc caggttgtcg accttcatgt ttacgtgaaa cgccgttacc gcgcgaaaat
      241 tcttctgcat ggtgctgatg acctgctgcg cggtcggctt cgcgcccagg ttgctgaccg
      301 gcgcggcggg agtgtgctgc cagggaaggc ggcagcttga tagaaggagc gcgcttgcca
      361 tgcagagcgc cacaagggcc agcgagcggc caaacgaacg ttgaattgaa cccatatgag
      421 atagcctccc caatagtagt ctaccaacct tgaaatgacc ggtaggggcg agcctttatg
      481 gtcgcccgcc ccttgaggca cccaaacatt tggtgaggca caccgggcga ccataaaggc
      541 tcgccccaac cggtcatgaa aaatggaaag cagtgcttag aaatgccttg ctgaaacaaa
      601 tcaacgcttg ctgttgacct ttcggggcaa tcaggcagga gaggcaggaa gggtggtact
      661 ttgtggctta tagcttttcc aaccgggcga agtcgaggct gaggcgtttt ttgccatgtt
      721 tgccgtgaaa ctgcacttcg acgaattcgg tctgctgctc catttcgctt ttgaggatga
      781 tgccttcgcc gaaggtggcg tggcgcaccc tgtcgcctgg cttgaactgc tggctctgcg
      841 gcctgggagc cggggatgat gttggcagag gcggcggcac cggcgaaggc ctggctgggg
      901 caggcagggg cgacgatgtg gaagggcgcg cgggcggcga cgaactggtc ggtggtttgc
      961 cggagccgaa gacgcgacct gtttcgcgcg cagcatagct gtcctggttg tagtcgtccc
     1021 acgaggagcg gcgggcaggc gcagtattag agcgggcagt tgatgaagag aaggagccag
     1081 cgccgccgga ttgggtgacg agcttcgtgg gaatgtcgtc gaggaaacgc gatggctgcg
     1141 tcggcacgac atcgccgaaa tacgagcgac gccgggcgcg cagcaggtag aggcgacgca
     1201 tggcgcgcgt gaagccgacg taggccaggc ggcgctcttc ttcgagctgt tccggcttat
     1261 cgaccgagcg ggcgtggggc agcgagcctt cgtccatgcc gatgatgaag accaccgggt
     1321 actccagccc tttggcggcg tgcagcgtga tgagggtcac ggcgtcgcgg ttctcctcgg
     1381 tgatgagctt gccgtcttcg cccgtctggg tggtatccgc gccgcccacg agcgccacgt
     1441 tttcgaggaa gagttccagc gcggtgcgcg tctcgatctc ggcatagtcg tcggcgacgc
     1501 ggcgcagctc cagcacgttg ctccaacgct cgtcgccgtc ggcctgctcg cgcagttcag
     1561 gccccaggcc gctgcgctcg gcgatgcggt cgatcaactc cggcagttgc agttcttcca
     1621 tggcctgctg gaaatccttc atcagcgcgc cgaaggtggc cagcgcctgc tttgccgact
     1681 tgccgagcgt tggatggtcc gcgatgcgct ggatggcctc gtagggcgtg ctatgctcgg
     1741 cggtggccca ctgctgcaac tcgcccagcg tcttcgggcc gatcttgcgg ttggggacgt
     1801 tgatgatgcg ccgcaggctc aggtcgtcat acggattgag cagcaggcgc aggtaagcta
     1861 ccatgtcctt gatttcttta cgctcataga acttgcggct gccgatgacc ttgtagggga
     1921 tgttcaggcg catgaactgc tcttccaggg cgcgggactg cgcgttggtg cgatacatga
     1981 cagcgatgtc gccgcgccgc tcgacttcgc cgcgcaacag gaggcgctgc gactcgatgc
     2041 cgacatattc ggcctcgctc tcctcgtcgt atgcttcgtg cataatgacc ttttcgccct
     2101 gaccgagcgc cgtccagagg cgtttgtcct tgcgcaggcg gttgcgctgc acgacgttct
     2161 gcgcggcgtc caggatggtc tgggtggagc gatagttttg ctcgagcagg atgacctgcg
     2221 tgtgggggaa gtcgcgctcg aagcggatca cgttttccac gctcgcgccg cgccaggtgt
     2281 agatcatctg gtcgtcgtcg cccaccacgc agacgttgcc caggccctcg tgccggtcgt
     2341 cggtgccgaa accaagctgg cggatgagct tgtactgcgg caggttgcag tcctggaact
     2401 catcgacgtg gatgtactcc cagcgccgct ggtagcggcg cagcacttcc ggttcgcgcc
     2461 gccagagctg ctcggtgagc aggaggaggt catcgaaatc gacgctgttg ttgtcgcgca
     2521 tcagcttctg gtatttctta tagacgcgcg ctgctacctc ttcggtatac ttggcggcct
     2581 tctcggccat ctggtcgggg ttctgcatgt cgttcttggc gcgcgagatc atggcctgca
     2641 tattggccgg tttgtactgc ttctcgtcga ggttcaaatc cctgacggcc tgtttgatga
     2701 gcgtggtctg atctccggta tcgaggatga cgaacgagcg gttgaggccg aggggcgcga
     2761 ggtagtcggc ctcgatgcgc aagacgcgcg cgcagatggc gtggaaggtg ccgagcatca
     2821 tctccttcga ctcgttgacg cctaccagct tttccagccg ctcgcgcatc tcctttgccg
     2881 ccttgttggt gaaggtgacg gccaggatgc gccagggcga gacctgctcg tgctggacga
     2941 tgtaggcgat gcggtgggta atcacgcgcg tcttgccgct gcccggtccc gccaggatta
     3001 acaacggccc ctgcgtcgtc gtgaccgcct gccgctgtgg ctcattcaaa cccgccagta
     3061 aatcttgctg ttccaacgtg ctcatatgct cgtcaacttc cctgcctatg tggtagtgga
     3121 tgtgtggtgt aaagtcctgg ctattgtacc acaggcggca aaacgatgtg gactttttta
     3181 ggagaaatgg gagacagaga tggaatcgct tgtggatcat gaatattctt tcattatcgc
     3241 ataaatcggt taatagaagc tatagagcat gattacagca tgtaacgtat ttgcttgctt
     3301 gaagaaacaa caaatgaaga aagatggaag atgaatgatt tgtggggtat ctatgaacaa
     3361 caattcacct tattcgtatc agagtatccc aaaacgtttc attttgtaat tgatgtcctt
     3421 ttattatgtc tagtatcatt aattgatatt acaataattt catataacaa aattagta
//
LOCUS       DASZAK010001185         2270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_242952, whole genome shotgun sequence.
ACCESSION   DASZAK010001185 DASZAK010000000
VERSION     DASZAK010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242952"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VGT44_20930"
     CDS             complement(<1..732)
                     /locus_tag="VGT44_20930"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583336.1"
                     /translation="MAETILTNTPTPEKEPPQERSRKSFLSRLGSIFLRQREASILII
                     AIILIIYFQFSSSNFLTSDNISTLFGFIYATAIIAAGEAMLLICGEIDLSVGQVSALA
                     PFIMYFVYQAGLPLWVGILAGLLVSAVVGLFNGIVTVWFKIPSLISTLGMLFLLTGIT
                     LTLSGGVPVLTPSAGLLNQVMGANAYWEITWAIAVMIVVQIILSFTRWGKHTVATGGN
                     LLGASEAGVNVSRVKIGNFMLASTLA"
     gene            complement(967..2025)
                     /locus_tag="VGT44_20935"
     CDS             complement(967..2025)
                     /locus_tag="VGT44_20935"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2583337.1"
                     /translation="MPIKRRRLLQTAVGSSAGAAILAACGATNPTTPSGSSSSGGGGN
                     FPSHPKWNFVFVNHVTTNPFFVPTQYGIQDACALVNCTYQWTGSDNSVVADMVNSMNA
                     AISAKADGIAVAIIDATAFNTPVQNALSAGIPVVSYNADAPASSNNARLAYIGQDLYQ
                     SGVALGNRIVSLVPSGDIPGFIATPGSLNIQPRIDGAKAAIQASGKSINFVEIATTAT
                     VNQELSVIEAYYLGHQNLKGMVAVDAGSTQGVSEVMEKYGLAAKGIHAGGYDLLPKTL
                     QEIQAGNLDFTIDQQPYLQGFIPVLQLFLYKLSGGLMFPAETDTGLLFVTKNNVGPYL
                     NTQTRFEGSSSQQKVVTA"
BASE COUNT          547 a          623 c          604 g          496 t
ORIGIN      
        1 cgccagcgta ctcgccagca taaagtttcc aattttgacc cggctcacat tgacaccggc
       61 ctcgctcgct ccaagcagat tcccgcccgt cgcgacggta tgcttacccc agcgtgtgaa
      121 ggagaggatg atctgcacga caatcatcac cgcgatagcc caggtgattt cccagtaggc
      181 attcgcaccc atcacctggt tgagcagccc ggcagaagga gtaagcaccg gcacaccccc
      241 cgaaagagtc agtgtgatac cggtcaacag aaagagcatt cccagcgtgg agatcagcga
      301 ggggatcttg aaccagaccg tcacaatgcc attgaacagg ccaacgacgg cagagacgag
      361 cagcccagcg agaatgccca cccacaaagg caggcccgcc tggtagacga agtacatgat
      421 aaaaggggcc agggcagaca cctggccgac cgacaggtcg atttcgccac agatgagcag
      481 catggcttcg ccggcggcga tgattgccgt ggcgtagata aatccgaaga gggtgctgat
      541 gttgtcagac gtcaagaaat tcgaactgga aaactggaaa tagatgatca ggatgatagc
      601 aataattaaa atactcgctt cgcgctgccg caaaaagatg ctgcccagcc ttgacaggaa
      661 gctctttctt gaacgttctt gtggcggctc tttctccggc gtcggagtat tcgtaagaat
      721 agtctcggcc atacgcccgc tcctttacac aatcgaacga tcatcgttcg caagcagaat
      781 cgatacctac cccttcaagc taaagccctt atgatcgctt tgtagtatac attactgcaa
      841 tagtaatccg gctaaaccag aaagtaaagc tccctgtgga tgtctgcatg aatcattgca
      901 gcttcacgca gacactcaca ggaaagctga gaataaaccg ctgccgagcg gtcttcaacc
      961 aaaaggttac gcggtcacaa ccttttgttg gctcgagctt ccttcgaagc gcgtctgggt
     1021 attcaggtaa gggccaacgt tgttcttagt gacgaagagc agacccgtgt ccgtttccgc
     1081 cgggaacatc agtccacccg acagcttata caggaaaagc tgcaagacgg gaatgaaccc
     1141 ctggagatac ggctgctggt caatggtgaa atcgagattg cccgcctgta tctcctgaag
     1201 cgtcttgggc agcaggtcat aaccaccagc atgaatgccc ttggcggcaa ggccgtactt
     1261 ttccatgacc tcggacacac cctgggtgct gcccgcgtca accgcgacca tacctttcaa
     1321 gttttggtgg cccaggtagt aggcttcaat gacggagagt tcctggttga ccgtcgcggt
     1381 tgttgcgatc tcgacgaagt tgatcgattt gcctgaagcc tgaattgccg cctttgcccc
     1441 gtcgatgcga ggctggatgt tcaatgatcc aggggtggca atgaatcccg ggatatctcc
     1501 gctgggaacc agcgagacga tgcgattgcc caatgcgaca ccggattggt agaggtcctg
     1561 accaatgtac gccaggcgcg cgttattgct gctggctggg gcatcggcgt tataggagac
     1621 aacgggaatg ccagcagaca acgcattttg tacaggtgtg ttgaacgctg tcgcatcgat
     1681 gatagcgaca gcaataccat cagctttggc cgagatcgcc gcgttcattg aatttaccat
     1741 atccgcgacg acgctattgt ctgagcctgt ccactggtag gtgcaattga cgagtgcgca
     1801 cgcgtcctgg atgccgtact gggtcggcac aaagaacggg ttggttgtta cgtgattgac
     1861 gaagacaaag ttccacttgg gatggctggg gaagttcccg ccccctccag acgaacttga
     1921 accactcggg gtcgttggat tagtggcgcc acaggctgcc agaattgccg cgcccgccga
     1981 acttcccact gctgtttgca gcaaacggcg acgtttgatg ggcaatcgcg aaaaatactc
     2041 cctaaagtta gtgtctgcca tgtgtttacc tccgcttaca gacgtttgtt gtcctcggta
     2101 gtcgaacggt aaaaagctaa ccagcgcgaa ccaactcctt cgatcggagc cggttgacac
     2161 caacatctct tctgcgttca tcctcctttc ctgaaaacta ttgcccctat cgctcgatgg
     2221 cgatccaaca gggcaatttt gctttattct cctctagttg cgacgcgact
//
LOCUS       DASZAK010001186         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_206147, whole genome shotgun sequence.
ACCESSION   DASZAK010001186 DASZAK010000000
VERSION     DASZAK010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206147"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VGT44_20940"
     CDS             <1..868
                     /locus_tag="VGT44_20940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2583338.1"
                     /translation="RYLELIERNDRETNSKPRVRLYTHHRVVPGEGEGFISCPDGRKR
                     IRIEALQEFDFKGQFVRVRVPGSEVIDLGVPELFMIAQGFSSADAKRLGFEQHDVTVD
                     HKDGRGPVVAQADFLAGLIEVLVGGRLRRRISSAFDQDGREYWVRQIAVGHENDPEVG
                     WVLVQVPDFKTFDPIEAGLLPEGTDPTSPEFFASYQQLIYDYYMKEAADILDIPREEL
                     RKVQMIYGPTLFSLIERVGDDALIAANGVVAGDSFGNGHFLTSGGAMTGMIGHSARPR
                     ILAGAGCRHLTR"
     gene            924..1742
                     /locus_tag="VGT44_20945"
     CDS             924..1742
                     /locus_tag="VGT44_20945"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2583339.1"
                     /translation="MSATEYSQAVPINFGAERIKQISKASGADPNARANSIDAARRQR
                     HALIPLDPSNWRRLFVRNGRVLSALPELEENHPLQRPEREASLLVVMQSLPFLQKRVS
                     EQPGTDQGLAVGEQTLLRPKHKREQDLAVGDETIIMPKLSIGPVAPESAKIYAFLDIQ
                     QNEQVVQRIAIKENQVVVGRVDPSRGIAPEIDLTPFDISNTVSRQHARIRVGKDFFSI
                     EDLKSRNKTRLGESTLTPLKPEALQNGDVVSFGAVKATFRLLGTSKLPVSWSQS"
BASE COUNT          502 a          645 c          685 g          479 t
ORIGIN      
        1 ccgctacctg gaactgattg aacgaaatga tcgggaaacc aacagcaagc cccgggtgcg
       61 gctgtacact caccaccgtg tggtcccggg tgagggcgag ggattcatct cctgccccga
      121 tggtcgcaag cgcattcgca tcgaggccct ccaggagttc gacttcaagg ggcagttcgt
      181 ccgtgtgcgg gtaccaggca gtgaggtgat tgacctgggt gtacccgagc tattcatgat
      241 cgcccagggc ttcagcagtg ctgacgcgaa acgcctggga ttcgagcaac atgacgtgac
      301 ggtcgatcat aaagacgggc gtggacccgt ggtggcgcag gcggatttcc tggccggtct
      361 gatcgaagtc ctcgtcggcg ggcgcctgcg ccggcgcatc tcgtcagcct tcgaccagga
      421 tggcagggaa tactgggtgc ggcagattgc tgtgggccat gagaatgatc cagaggtggg
      481 ttgggtactg gtgcaggtgc cggacttcaa aaccttcgat cccatcgaag cggggctcct
      541 tccggaaggg actgacccca cttcgcccga attcttcgct tcctaccagc agttgatcta
      601 cgactactac atgaaggagg ccgcggacat cctggatatt cccagagaag agctgaggaa
      661 ggtgcagatg atctatgggc caacgttgtt cagtctcatc gaacgggtgg gagacgatgc
      721 gctaattgct gccaatggtg tcgtcgccgg tgattccttc ggcaatggtc acttcttgac
      781 cagtggtggg gcgatgacgg gaatgatcgg acacagcgcg cgtcctcgaa tactggcagg
      841 cgcgggatgc aggcacctca ccagatgacg ctcttcgccg gctcgccgac cgcatcagag
      901 aagacactca tgcctggctt caggtgagcg cgacggaata cagccaggca gtcccgatca
      961 acttcggcgc tgaacgcatc aaacagatca gtaaggcgag cggtgccgac cctaatgcgc
     1021 gggctaactc tattgacgcc gctcgacgcc agcgccacgc gctgattccg ctggatccat
     1081 ccaactggag aaggttattc gtgcgcaatg gaagggtgct ctcggcgctg ccggagcttg
     1141 aagaaaacca tccgctgcag cgtcctgagc gcgaggctag cctgcttgtc gtgatgcaat
     1201 cgttgccttt cctgcaaaaa cgcgtcagcg aacagcctgg cacagatcag ggcctggcag
     1261 taggtgagca gaccctgctc agacccaaac ataagagaga gcaggacctg gcagtaggcg
     1321 atgagaccat catcatgccc aaactcagca tcgggccggt ggcgccagag agcgcaaaaa
     1381 tctacgcatt tctggacatc cagcagaacg agcaggtggt gcagcgcatc gccatcaaag
     1441 agaaccaggt ggttgtcggg cgggtcgatc ccagccgggg aatcgcgccg gaaatcgacc
     1501 tcacaccgtt cgacatctcg aacaccgtct cacgccagca cgcgcggatt cgcgtgggga
     1561 aagacttctt ctctatcgaa gatctcaaga gtcgcaataa gacgcggctg ggcgagtcga
     1621 ccttaacccc gctcaagcca gaagcgctcc agaacggaga tgtggtcagc tttggtgcgg
     1681 tcaaggccac cttccgcctg cttggaacga gcaaactgcc cgtgtcctgg tcgcagtcgt
     1741 agctttatag gtcgagcttg ttggtacgct cggcctggtc atctcgcaaa cagtgatctg
     1801 atcgttagga acacgatgcc actttgttaa gcttcaggta tctacttccc agagactgga
     1861 agcgtcaggc caaaaaggtt ggcgagcgcc tgggtcattt cttttaatac cgttagttca
     1921 accgtgaagt agttagtacc ggatggacgg cgggatacgc atgcagccgc gctgtcatac
     1981 ggcttttctg cctcgtcttc taatgccacg tccataaccc gtgtaactaa cattgtcgag
     2041 agggagaaac cggtgtgctg atgcgctccc tctcattgtt tgttgtgcaa gcttgtttca
     2101 aacaagcgat ggaaagaaca tcctcatcga tagtggactg ccagcggact acaccccacc
     2161 accagggaca accagcattc cgtcaacaac cattgatcat cttctttgtc tgcttagatg
     2221 gactgctgtg gagcagctgt tgcctcgtct ccggtatttg gcgttccggt tggctcgatc
     2281 aagagcaggt gcgtttcttc ctctgctcgg g
//
LOCUS       DASZAK010001187         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_313971, whole genome shotgun sequence.
ACCESSION   DASZAK010001187 DASZAK010000000
VERSION     DASZAK010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313971"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /gene="nuoF"
                     /locus_tag="VGT44_20950"
     CDS             <1..1019
                     /gene="nuoF"
                     /locus_tag="VGT44_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEV2583340.1"
                     /translation="NPHQLIEGVIITSYACRVSTAFIYVRGELALAGRQVQRAIEEAH
                     AAGYIGKNILGSNYSLDLIMHRGAGAYICGEESALMESLEGRRGYPRLKPPFPAVVGL
                     YNGPTVINNTETLATIPAIIIHGAEWYASFGTEKSKGTRVFCLSGHVKKPGNYELPLG
                     TPLRTLIYDEQYGGGILDDKQLKAIIPGGSSTFILGADSVDTPLDFEAIAAAGSMLGS
                     GGVVVMHEDTCIVGAVLRMTEFYRDESCGKCTPCREGTYWLTEILHRIEHGHGEESDI
                     DLLMDICDNISGKSFCPLGDAATSPITSSIKLFREEYEYHIREGRCMAGAQRQDEEAM
                     LIGT"
     gene            1066..>2067
                     /locus_tag="VGT44_20955"
     CDS             1066..>2067
                     /locus_tag="VGT44_20955"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV2583341.1"
                     /translation="MPEEKKDELVHLTIDDIAVAVPPGTLVWAAAQQIGIEIPIYCYH
                     PKMPPLGACRMCFVEVEKMPKPPQTACTTPVSEGMVVHTNTEKVLKARRGTLEFLLIN
                     HPLDCPICDKGGECDLQDFTLRHGPGGTRFDLHKRHYPKPIPVSDNVMLDRERCILCQ
                     RCTRFSSEISMDNGLVMISRGYKMEVGTAPDHAFDSIFSGNTVEMCPVGALTAASFRF
                     KSRPWELKHTPSVCNNCSVGCNVRVDVRVDKVMRLMSRTNDDIDDGWLCDRGRWGFEF
                     VNSPQRLRTPLMRVHRNKKLEPATWDEACYFIATRLRGIIDKHGAQAVGGIGSTRTTN
                     "
BASE COUNT          503 a          621 c          527 g          416 t
ORIGIN      
        1 agaacccaca tcaactaatc gaaggcgtca tcatcaccag ctacgcctgt cgtgtttcaa
       61 ccgcctttat ttacgttcgt ggcgaattgg cactggcagg acgccaggtc caacgagcca
      121 ttgaagaagc acacgccgct ggctacatcg gcaagaatat ccttggcagc aactacagcc
      181 tggacctgat catgcatcgt ggtgctggcg catacatctg cggtgaggaa agcgccctga
      241 tggaatcgct cgaaggcagg cgcggctatc cacgtttgaa gccccctttt cccgccgtcg
      301 tcggcctgta caatggtcca actgtaatca ataacactga aacgctcgca accatcccgg
      361 cgatcatcat acatggagct gaatggtacg catcatttgg cactgagaag agcaagggca
      421 cacgcgtctt ctgcttaagt gggcacgtca agaagcccgg caactatgaa ttgccgctgg
      481 ggacaccgct acgcaccttg atctacgacg agcaatacgg tggaggcatc ctcgatgaca
      541 agcaactcaa agccatcatc ccgggcggat cgtctacctt catcctcggc gcagacagcg
      601 tagacacgcc cctcgacttt gaggccatcg ctgccgcagg ctccatgctc ggctccggtg
      661 gagtcgtcgt catgcacgaa gatacctgca tcgttggtgc tgtgttgcgc atgaccgagt
      721 tctaccgcga tgaatcctgc ggcaaatgca ctccctgtcg cgagggcacc tactggttaa
      781 ccgagatcct ccatcgcatc gaacatggcc atggcgagga gagcgacatc gacctactta
      841 tggacatctg cgataatatc agtggcaagt cattctgtcc cttaggcgac gccgctacca
      901 gcccgattac cagcagcatc aaactcttcc gagaagaata cgaatatcac atccgcgaag
      961 gacgctgcat ggccggtgct cagcgccagg acgaggaagc catgctcata ggcacttaat
     1021 ctttacaagc aggtgctcct gctaaacaat aacaagagaa aacatatgcc agaagagaaa
     1081 aaagacgaac tcgtacacct gacaatagac gacatagccg tcgcagtccc gcccggaaca
     1141 ttggtctggg cggcagcaca acagatcggc attgagatcc ctatctattg ctaccatccc
     1201 aaaatgccgc cactgggtgc ttgccgcatg tgcttcgtcg aggtcgaaaa aatgcccaag
     1261 ccaccacaga ccgcctgcac cacacccgtc agcgaaggca tggttgtcca caccaacacc
     1321 gaaaaggtgc tcaaagctcg caggggaacc ctggaattct tactcatcaa ccatcctctt
     1381 gattgcccca tctgtgacaa gggcggcgag tgtgatttgc aagacttcac cctgcgtcac
     1441 ggacccggcg gcacacgctt cgacctccac aagcgtcatt atcccaagcc gattcccgtc
     1501 agcgacaacg ttatgctcga ccgcgagcgt tgcattctat gtcagcgctg cacgcgcttc
     1561 agttcagaga tatcaatgga caatggcctc gtcatgatct cacgcggcta caaaatggaa
     1621 gttggcacag ctcccgacca cgcctttgac tcgatcttct cgggcaatac tgtcgaaatg
     1681 tgccctgtgg gtgccctgac ggccgcaagc ttccgcttca aatcgcggcc atgggagttg
     1741 aagcacacac cgagcgtctg caacaattgt agtgtggggt gcaatgtgcg cgtcgatgtg
     1801 cgcgtcgaca aagtcatgcg cttgatgtcg cgcaccaacg atgacattga cgatggctgg
     1861 ctctgtgacc gcgggcgctg gggcttcgag tttgtcaaca gcccgcaacg gctacgcact
     1921 cccctcatgc gcgtccaccg caataaaaaa ttagagccag caacgtggga tgaggcttgc
     1981 tacttcattg ccacacgtct cagaggcatt attgataagc atggtgctca ggccgttggc
     2041 ggcatcggct caacccgtac taccaac
//
LOCUS       DASZAK010001188         6644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_492070, whole genome shotgun sequence.
ACCESSION   DASZAK010001188 DASZAK010000000
VERSION     DASZAK010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492070"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VGT44_20960"
     CDS             complement(<1..847)
                     /locus_tag="VGT44_20960"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2583342.1"
                     /translation="MAEFYTVPNIYSDISNQSQGYFAFRFHCQRCGTALESLPIRSTV
                     ATTQNIMDVGIGMLGGFWGRAAQEGEQMFGAKWHQEQAQALTKAWQQVQHEFHTCPKC
                     HSTVCMRCWNTALNLCTGCAPDLKADGASFQHELNIDAQRQQIQQGYQAPQFNVSAVP
                     SAVTPDMVVQPGQQRPSLPPAQQAYPVQSGQPAYPAQQPMPHTPQQQGAPVGNSPLAG
                     SRFDTPGFPRQVMCTVCRKMGPPGKFCDNCGTKLPMPDLFCPNCSEPVQPTTRFCPEC
                     GTKLGQ"
     gene            complement(934..1779)
                     /locus_tag="VGT44_20965"
     CDS             complement(934..1779)
                     /locus_tag="VGT44_20965"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV2583343.1"
                     /translation="MGVRIVTDSTADIPREQAEALGIAVVPLTVFFGDEAYLDGIELD
                     NAGFYRKLAASRDLPRTSQPSPASFQETYTRLIDEGADAILSVHLSAKLSGTYQSART
                     ARDSLPEDIRRIPIEVVDSQSISVGMSRAILQAAQDARDGQSLEEIKSLLLDQLARTR
                     ILAVLDTLEYVKRGGRIGGARAMLGNMLSVKPIISLKDGEVVPVEQPRTRSKAYTRVA
                     EILKASGKIEYVAIAEASEEVGQQLAQVIKTSYTGELPIYRLGAVLGTHTGPGTAAIA
                     YVIAS"
     gene            complement(1792..3600)
                     /locus_tag="VGT44_20970"
     CDS             complement(1792..3600)
                     /locus_tag="VGT44_20970"
                     /inference="COORDINATES: protein motif:HMM:TIGR03599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV2583344.1"
                     /translation="MQEPPTGPTSQSNRAEASVVSAEPTTAVAPAAHTAYPVRPQRQR
                     RISVFDGQDLKKAMLAGAAWLEEHRDAINALNVFPVPDGDTGSNMSATMQAAVRDLPA
                     SKDTSAGVIAAKISRGALLGARGNSGVILSQILRGLAQSLDKKQTFTAHDLADALKAA
                     QQLAYRAVLKPVEGTILTVIRETAQAAQEGASRGDDLVALMQEIVIAARQSVARTPDL
                     LPILKQAGVVDSGGQGLCTILEGIWRYVRGEATGIVESAQETLYAAGKTGRTTGQAAE
                     PVVKKGRVTIEEEFGYEVVFLLSGKRLDVEAIRQRIIDMGGVSTVVAGDEKLLKVHTH
                     TEWPGKILDYGVSLGSLLDINIENLQEQSLTYAAASAAEHAGDEADQPQARQISTVAV
                     VSGPGFEQVFEGLSVSSIVPGGQTMNPSTEELLAAVEACPSNDVILLPNNGNVILSAR
                     QVVNLTHKRVYIVPTDTMPQGIAALTRFNFEADFEANCAAMSEAASSIHTAEITTAVR
                     SVQIGGVRVREGDFIGLIDGNLAIAGKDMQRVIRETLEHMDIDRFEIATLYFGNDVTE
                     AEAEATARRIKEQHSQLEIEVVNGGQPHYAYIISAE"
     gene            complement(3620..4003)
                     /locus_tag="VGT44_20975"
     CDS             complement(3620..4003)
                     /locus_tag="VGT44_20975"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV2583345.1"
                     /translation="MSRTSAIQPQHGIRPLGKIEVLPNAIHSIAVQAISECYGVVGIA
                     APRLHSGQAILLEAGQINQGVQVRIVDDQIMLEVYVVLEYGLRISEIAHNIMSGVKFA
                     VEKMLGMPVARVNVNVQGLSHSANS"
     gene            4235..4429
                     /gene="rpmB"
                     /locus_tag="VGT44_20980"
     CDS             4235..4429
                     /gene="rpmB"
                     /locus_tag="VGT44_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV2583346.1"
                     /translation="MSGRCEICERKPMHGNNSNFSQKRSRRRFVLNVQRRRIEINGTT
                     KTVNICTRCLRTMNKLPKAR"
     gene            complement(4478..5623)
                     /gene="rlmN"
                     /locus_tag="VGT44_20985"
     CDS             complement(4478..5623)
                     /gene="rlmN"
                     /locus_tag="VGT44_20985"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV2583347.1"
                     /translation="MSLSPITLNTTQKTDLLSLSLPQLQQWLAERGEPAFRAKQIYQW
                     LYQQLVSDFAAMTNLPLALRSRLAEEASIGPVTVRSEQHSKDDRTRKILLELEDGKLI
                     ESVLMLYPPLGEQSARATVCVSSQAGCSYGCTFCATGQMGFDRHLSAGEIVAQVLHFA
                     RELRAAPWTAKGLPGSAPIDHITNIVLMGMGEPLHNYDNVLQALRILNSPEGFCLGAR
                     HMTVSTVGLVPAIRKLSQEQLQVNLAISLHAPTDELRSRTMPINRKYPIAQLIAACQD
                     YIAATHRQVTFEYVLLAGVNDTPDYAHKLGELLAPLKQFAHVNCIPVNVTAADYRPPS
                     GETIRAFRDILYAHTVSNSVRAERGDDIAAACGQLRTRFELRKKQTQ"
     gene            complement(6058..>6644)
                     /locus_tag="VGT44_20990"
     CDS             complement(6058..>6644)
                     /locus_tag="VGT44_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583348.1"
                     /translation="RKLASNSMSAVIGLVHDSHRIALLPGDLDELGLENLLMDQGNIE
                     ASVLIFPHHGGSANSRDNYNFSRKLCSLVNPRLVIFSFERGRLYGKHRTENPRDDIMR
                     GIISSCPNAHIMCTQLSGKCAAKNPNSDFSHLTNLPALGSTNDSCCGGTMRIRINGNQ
                     TTYSPQRSSHAAFISDSSNVPTPMCLLHFNEVQI"
BASE COUNT         1303 a         1822 c         2007 g         1512 t
ORIGIN      
        1 cctgccccaa ctttgtgccg cattcggggc agaagcgcgt ggtcggttgg accggctcgg
       61 aacagttcgg gcagaacagg tccggcatgg ggagtttcgt gccgcagttg tcgcaaaact
      121 tgcccggcgg ccccattttg cggcagaccg tacacatcac ctgcctgggg aaaccgggtg
      181 tgtcgaagcg cgaaccagca agcgggctgt tgcccaccgg cgcgccttgc tgttgtggag
      241 tatgaggcat gggttgctga gccgggtacg ccggttgccc tgattgaacg gggtatgcct
      301 gctgagcagg cggcagcgat gggcgttgct ggcccggctg taccaccata tccggcgtca
      361 ccgccgaggg aaccgcgctg acgttgaact gcggcgcctg gtagccctgc tggatctgct
      421 ggcgctgcgc atcgatgttc aactcgtgct ggaagctggc cccgtcagcc ttgaggtcgg
      481 gcgcgcagcc ggtacacagg ttgagcgcgg tattccagca gcgcatacag acggtcgaat
      541 gacattttgg gcaggtatga aattcgtgct gtacttgctg ccaggctttc gtgagggcct
      601 gagcctgctc ctggtgccac ttcgcgccaa acatctgctc gccctcctgg gcagcgcggc
      661 cccagaagcc gccgagcata ccaatgccaa catccataat gttctgggtt gtagctaccg
      721 tcgagcggat gggcaaagac tctaacgcgg tgccgcagcg ctggcaatga aagcggaaag
      781 cgaaatagcc ctgtgactgg ttacttatgt ctgaatagat atttggcaca gtatagaatt
      841 ctgccataga tttctccttt gtaaagtgtt cggcggaaac gcggtaataa acaatagcaa
      901 atcaagcaag gctcgcccct acggacatcc ttgttacgat gcgataacat aggcgatggc
      961 ggcggttcct ggcccggtat gggtgccaag caccgcgccc agccggtaaa tcggcagttc
     1021 acccgtatag ctggttttga tgacttgcgc cagttgctgg ccgacttcct cgcttgcttc
     1081 ggcgatggcg acatactcga tcttgccgga tgccttcagg atttcggcga cacgtgtata
     1141 ggctttgctg cgagtgcgcg gctgttcgac agggacgacc tcgccatctt tcaaagaaat
     1201 gatgggtttg acgctcaaca tgttgccgag catggccctg gccccgccga tgcgcccgcc
     1261 gcgcttgacg tattccaggg tatccagcac agccaggatg cgcgtgcgcg cgagctgatc
     1321 gagcaggagg ctcttgatct cctcgaggct ctgcccatcg cgcgcatcct gtgctgcctg
     1381 gagaatggcg cgcgacatac cgacgctgat gctttgcgaa tccaccactt caatcggaat
     1441 tcgccggata tcttccggta gcgaatcgcg ggcggtgcgg gcggattggt aggtgccgct
     1501 cagtttggcg gagagatgca ccgagaggat agcatcagcg ccttcgtcga tgaggcgcgt
     1561 ataggtttcc tgaaagctgg cgggcgatgg ctgcgaagta cgcggcagat ccctgctggc
     1621 tgccagtttg cggtaaaagc ccgcgttatc cagttcgatg ccatccaggt aagcttcatc
     1681 gccgaagaag accgtgagcg gcacgacagc gatgccgagc gcctcggcct gctcgcgtgg
     1741 gatgtcggcg gtgctatcgg tcacaatacg aacacccata ggaacctccg gttattccgc
     1801 agaaataatg taggcgtaat gcggctgtcc tccgttgaca acctcgattt cgagctgcga
     1861 gtgctgttct ttgatacggc gcgcggtggc ttccgcctct gcttcggtca catcgttgcc
     1921 aaagtagagc gtggcgatct cgaagcgatc gatatccata tgttcgagcg tctcgcgaat
     1981 gacgcgctgc atatctttcc cggcaatggc caggttgccg tcgatcaggc cgataaaatc
     2041 gccttcgcgc acacgcacgc ctcctatttg gacgctgcgc acggcggtgg tgatttcagc
     2101 cgtgtggatg ctgctggccg cttcgctcat ggccgcgcag ttcgcctcga aatcggcctc
     2161 gaagttaaaa cgagtcagcg cggcgatgcc ttgtggcatg gtatcagtgg gaacgatgta
     2221 gacgcgcttg tgggtcagat tcacaacctg tcgcgcgctg aggatgacgt tgccgttatt
     2281 cggcaggaga attacgtcgt tggatgggca ggcttcaaca gcggcgagca attcttcggt
     2341 gctggggttc atagtttgcc cgcctggtac aatggaactc acgctcagtc cctcgaagac
     2401 ctgttcgaag ccgggaccgg agacgacggc caccgtcgaa atctgccgcg cttgtggttg
     2461 gtctgcttcg tctcctgcgt gttcggcggc gctggctgcc gcgtaggtca gactctgctc
     2521 ctggagattc tcgatattga tgtcgagcag gctgcccagg ctgacgccgt aatccagaat
     2581 tttgccgggc cactcggtat gcgtatgcac tttcagcagc ttctcatcgc ccgcgacgac
     2641 ggtagagacg ccccccatat cgatgatgcg ctggcggatg gcttccacat ccaggcgctt
     2701 gccgctcagc aggaagacga cttcgtagcc aaactcttcc tcaatcgtga cgcgcccttt
     2761 tttgacgacc ggctcggctg cctgtcctgt tgtccgcccc gttttgcctg ccgcgtacag
     2821 cgtctcctgc gcgctctcga cgatgcccgt tgcttcgcca cgcacgtaac gccagatgcc
     2881 ctccaggatg gtgcaaagcc cctggccgcc agaatcgacg acgcccgcct gtttgagtat
     2941 gggcaacagg tcgggggtgc gcgcgacgga ttgacgcgcc gcgatgacaa tttcctgcat
     3001 gagcgcgacc aggtcgtcgc cgcgactcgc gccctcctgg gctgcctgcg ccgtttcgcg
     3061 gatgaccgtc aggatggttc cctcgaccgg cttgaggacg gcgcgatagg cgagctgctg
     3121 ggccgctttg agcgcgtcgg cgaggtcatg cgcggtgaag gtctgtttct tatcaaggct
     3181 ctgtgccaac ccgcgcagaa tttgcgagag gatcacgccg gagttgccgc gcgcgccgag
     3241 cagcgcccca cgcgagattt tggcggcgat gacgccagcc gaggtatcct tgctggcggg
     3301 gagatcgcgc acggcggctt gcatggtggc cgacatattc gagccggtgt cgccgtccgg
     3361 cacggggaag acgttgagcg cgttgatggc gtcgcgatgc tcttccagcc aggcagcccc
     3421 ggccagcatc gctttcttga gatcctgccc atcgaacaca ctgatacgac gctggcgctg
     3481 gggtcgcaca ggataggcag tatgcgcggc gggcgctacg gcggtagtgg gttcagcgga
     3541 tacaacggat gcctccgccc ggttcgattg acttgtggga ccagttggtg gttcctgcat
     3601 agttaacgta acgtccttgt tatgaattcg cgctgtggct cagtccctgc acattgacgt
     3661 tgacccgcgc aaccggcatc cccagcatct tttcgacggc gaatttcact ccgctcataa
     3721 tgttgtgggc aatttctgat atgcgcaggc cgtactccag cacaacgtag acttcgagca
     3781 tgatctggtc atctacaatg cgcacctgca cgccctggtt gatctgcccc gcctccagca
     3841 ggatagcctg tccgctgtgc aggcgcggcg cggcgatgcc cacgactcca tagcattcgc
     3901 tgatagcctg caccgcgatg ctgtggatgg cgttaggcag cacttcgatc ttgcccagtg
     3961 ggcgaattcc atgctgtggt tgaatagccg atgttcgcga catacgtctt tgctccacaa
     4021 aaatttccga aaacccttgc cagatgaggc caatagtgct atactcgcag ggtgtgctta
     4081 gaatgcctca cgtccagtta cgtgtgtccg agcgtgtgac tccggttctg agaaacatct
     4141 gcattgtatc aaaagcacca gtgataatca agggaggggc gtacccgcgc ggtcgccgtt
     4201 ggaacctcat caataaagag aggaaatgaa gatcatgtca ggtagatgtg aaatatgcga
     4261 gcgcaagccc atgcatggca ataattcgaa cttctcgcag aaacgctcgc gccgccgctt
     4321 tgtgctgaac gtgcaacgcc gccgcatcga aatcaatggg acgaccaaga cggtgaatat
     4381 ctgcacccgc tgcctgcgta ccatgaataa gctgccgaag gcgcggtagg ggaagcagac
     4441 ccaataaaac cccccatcgg tctaccgatg gggggtttta ttgggtctgc ttctttctca
     4501 gctcaaacct cgttcgtaac tgcccgcagg cggcggcgat gtcgtcacct cgttcggcgc
     4561 gcacgctgtt gctgacggtg tgggcgtaga ggatgtcgcg aaaggcgcgg atggtctcgc
     4621 cgctcggcgg gcgataatcg gcggcggtga cgttgaccgg gatgcagttg acgtgggcga
     4681 actgcttgag cggggcgagc agctcaccga gtttgtgcgc gtagtcgggg gtgtcgttga
     4741 cgccggccag cagcacgtat tcaaacgtga cctggcgatg agtggctgcg atgtaatcct
     4801 ggcaggcagc gatcagttgc gcgatgggat atttgcgatt gatcggcata gtgcggctgc
     4861 gcagttcgtc ggtcggcgcg tggagtgaga tggcgaggtt gacctgcaat tgctcctggc
     4921 tgagcttgcg gatggcggga acgaggccga ccgtcgagac ggtcatgtga cgcgcgccca
     4981 ggcagaagcc ctccgggctg ttgaggatgc gcagcgcctg caagacgttg tcgtagttgt
     5041 gcagcggctc gcccatgccc atcaacacga tgttggtgat atggtcaatc ggcgcgctgc
     5101 ccggcagccc tttcgccgtc cagggcgctg cgcgcagttc gcgcgcaaag tgcagcacct
     5161 gcgcgacgat ctcgcccgcg ctgaggtgac ggtcgaagcc catctggccc gtcgcgcaaa
     5221 acgtgcagcc atacgagcag cccgcctggg aggagacgca cacggtcgcc cgcgcgctct
     5281 gctcgcccag cggcgggtag agcatcagca cggactcgat cagcttgcca tcctccaact
     5341 ccagcaggat tttgcgcgtg cgatcatcct ttgaatgctg ctcgctgcgc acagtgaccg
     5401 gcccgatgct ggcctcttcc gccagtcggc tgcgcagcgc cagcggcagg ttggtcatag
     5461 ccgcaaagtc gctcacgagc tgctggtaga gccactggta gatctgcttc gcgcggaatg
     5521 ccggctcgcc gcgctcggcc agccattgct gcaattgcgg cagcgagaga ctgagcaggt
     5581 cagttttttg ggttgtattg agcgtgattg gtgatagaga catgggtgta ttatagcacg
     5641 acggtgatag aggttgctaa gtattcgtcg agtggctctc ggagcgcctc gacgattttt
     5701 ggtcggtgcc aatttgtcta ttgttgtcta ccgttgttcg cggttttctc tcggctttgc
     5761 tgtcagtttt gctgtgcgct ttggtctggt gatcgttgct cctatgactg ttctaaacgt
     5821 ggatggtggt acaagaaggt cgatttcatt acccctcttg actgactcat ggaatagacg
     5881 aaggtaagcg catggaaggc ttgtttcctg tccaccattg taaaccttta ataaccaaag
     5941 tcaatgggat tgactgtcaa cttgactgtc aaggcaaaag gatttagaca aattaatacc
     6001 ctggttctgc aatcttttgg agtgtgaacc aaggtagtag catgaatgta ggattggtta
     6061 tatctggact tcattaaagt gtaataagca cattggcgtg ggaacattac ttgaatcgga
     6121 aataaacgct gcatgtgaag agcgttgagg agagtaggtt gtttggttac cgtttattct
     6181 gattctcatg gttcccccac aacagctatc atttgtagag ccaagggcag gtaagttggt
     6241 aagatgacta aaatctgaat ttggattttt agcagcacat tttccagata actgagtaca
     6301 cataatatgg gcattggggc atgatgatat tatacctctc attatgtcat ctcttggatt
     6361 ttcagtgcgg tgcttaccgt acaagcgtcc tctttcaaaa gagaagatta ctaacctggg
     6421 gttaactaag ctgcatagtt ttcgggaaaa attataatta tctctactgt tggcagagcc
     6481 tccatgatgc gggaagatca aaacagatgc ttcaatattc ccttgatcca ttaaaaggtt
     6541 ttccaaacct aactcatcaa ggtctcctgg caataaagct atcctatgag agtcatgtac
     6601 caggcctatt actgccgaca tagagttaga tgctaatttg cgtc
//
LOCUS       DASZAK010001189         3678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_528788, whole genome shotgun sequence.
ACCESSION   DASZAK010001189 DASZAK010000000
VERSION     DASZAK010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528788"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VGT44_20995"
     CDS             <1..565
                     /locus_tag="VGT44_20995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583349.1"
                     /translation="HAARHERIEELLEVVALTQVAHRKLKTYSGGMKRRIGIAQALLN
                     DPALLIVDEPTAGLDPEERIRFRNLLSELGSNRTVLLSTHIVEDIAQTCQRLAILKGG
                     HVTFQGTATDALREAQGKVWIISTSGNKPEGNFTVVAALHLGTAMQYRVVGELSPRAG
                     AVAVEPTLEDSYVWLMREQHTPIRASI"
     gene            590..1369
                     /locus_tag="VGT44_21000"
     CDS             590..1369
                     /locus_tag="VGT44_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583350.1"
                     /translation="MNQFTTFLGALSYEFRMQIHRRSVWITFVALGLFFVVFHQPWYR
                     PITTPAGDAIVYWTGEVQSFLAIAVGILLADRLPRDRRTRVDEVLNTLPGAMSARVWG
                     KYLGSTFATLVPTFAVYSVGVGYIMYRWHAVETLPIALAAFATIALPGILFIGAFSIA
                     CPAILWVPVYQFLFVGYWFWGNLLPNFGIPTLSETILTPVGGYMCTGFFNPGGHEGVC
                     SPGIQGATALQGVESILLLLGIAVIVILLLPLFLKRRLSWG"
     gene            1429..2127
                     /locus_tag="VGT44_21005"
     CDS             1429..2127
                     /locus_tag="VGT44_21005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583351.1"
                     /translation="MATILLVEDAPDLAQIIIRELEANGYSALLASDGLTALALHAKE
                     HPELVILDWMLPRLSGLEVLRRLRQDSSTPVLMLTARGEETDRVVGLELGADDYLTKP
                     FNMRELIARVRAVLRRAELVRQMLITDQTAVGDRLSYGTLKLDAQAHNTLLDGAPLDL
                     SPTEFALLHLFLRNPGRAFSRAYLLDTVWDETYVGGDRSVDNTVLRLRKKLGSLGEAI
                     ETVWGIGYRLRPER"
     gene            2135..>3678
                     /locus_tag="VGT44_21010"
     CDS             2135..>3678
                     /locus_tag="VGT44_21010"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583352.1"
                     /translation="MPRSSLIFNMLPVRAILETLLAGLTLWLVLLLFRPLLASLIWRL
                     SLCLGIGVICVIFFALRLLRSAGSRQQILLYETALGGAICLIMSCLELITLLILIQGV
                     SLSPPWRDTNRPLLTALIALLLNLVVFFVTRSVIRLWFFWNQLRRRQLLWALTHAHLM
                     ILLLGAGLLLVVLETLVLLNSPDVFLIVSTTLGLIVLSITGLLLVLPLLALFSYLVIR
                     RTTQRLQTLAAATSALRQGNYAIRVPVGGEDEVARLQADFNGMAADLEHAMLALQEER
                     DRVAALLQARREMIATVSHELRTPVATLRGYLETILLHWEEPSNLTLHRDLEVMEEEV
                     LHLQFLVEELFTLARTDVGRLTLHCEPTDIGAIAQRVVETMAPLAWRTSKIEMIAEAP
                     AVLPPALVNANRLEQVLQNLLHNAVRHTAPGGIVAVMVTEEQTAITLQVKDTGEGIAA
                     ADLPHIWERFYQIEDTRTRTNGGVGLGLALVKEWIEEMSGTVTVESVVGEGSCFHIRL
                     PKVVEEG"
BASE COUNT          774 a          977 c         1059 g          868 t
ORIGIN      
        1 acacgccgcg cgccacgagc gcatagagga attgttggag gtagtagctt taacacaggt
       61 cgctcatcgc aagcttaaaa cctattctgg aggaatgaag cgtcgtattg ggatcgccca
      121 ggcgctactc aacgatcccg cgttattgat tgtcgatgag ccaacggccg ggcttgatcc
      181 agaagaacgc atccgttttc gcaacttgct atcggagctg ggcagcaacc ggactgtgct
      241 gctttcaacg catatcgtag aggatatcgc gcagacatgc cagaggctgg ccatcttgaa
      301 gggcggtcac gtcaccttcc agggaacagc gaccgatgcg ctacgtgagg cacaggggaa
      361 ggtctggatc atcagcacgt cgggaaacaa accggagggg aatttcaccg ttgttgccgc
      421 gctccacctg ggaactgcta tgcaatatcg cgtcgtcggc gaactttccc cacgagcagg
      481 ggcagtcgct gttgagccaa ctctagagga tagctatgtc tggttgatgc gagaacaaca
      541 tacacccatc agggcaagca tctaaagtag acacggaaag tagatattca tgaaccaatt
      601 tacaacattt ttgggcgcgc tctcctatga attccgtatg cagatacatc gtcgttccgt
      661 ctggataacg ttcgtcgccc tgggtctgtt tttcgtcgtg tttcatcagc cctggtaccg
      721 accgattaca accccggctg gggacgcgat tgtgtactgg acaggagaag tacagtcctt
      781 tctcgccatt gctgtaggga tattactggc agaccgcctg ccgcgagata ggcggacgag
      841 agttgacgaa gttctcaata ccttgcctgg cgcaatgagt gccagagtat gggggaaata
      901 cctgggtagc actttcgcca cgcttgttcc cacatttgca gtctacagcg ttggcgtagg
      961 gtacatcatg tatcgttggc acgccgtaga gacgctgccc attgccttgg cagcatttgc
     1021 gactatcgct cttcccggca tcctattcat cggggcattc tccatagcct gcccggccat
     1081 tctctgggta ccggtttacc agttcctctt cgtgggttac tggttctggg gcaacctgct
     1141 cccgaatttt ggcattccta ccctgagcga gacgattctt actcctgtag gtggatatat
     1201 gtgtacagga ttcttcaatc ctggaggaca cgagggagtg tgttcgccgg gaatccaggg
     1261 agcaaccgct ttgcaggggg ttgaaagtat cctgcttctg ttaggcatag ctgtgatagt
     1321 aatactgctt ttgccactgt tcctgaaaag acgactatcg tggggatagc agggtgatga
     1381 catagtgtat actgggacaa atacggaaac tgagtaggat tgcgcgaaat ggcaacgatt
     1441 ctcctggtcg aggacgcgcc tgacctggca cagatcatca tacgagaact ggaagcgaac
     1501 ggctacagcg cgctgctcgc gagcgatggt ctgacggcgc tcgcgttaca tgcaaaagag
     1561 caccctgaac tggtaatact ggactggatg cttcccagac tcagcgggtt agaggtgctg
     1621 cgccgactgc gacaggattc ctctacgccg gtgctgatgc tgacggcgcg tggggaagaa
     1681 acagatcgag tggtcggcct cgaactgggc gctgacgact atctcacaaa gccctttaat
     1741 atgcgcgagc tgatcgcgcg cgtgcgggca gtgctgcgtc gcgccgaact tgttcggcag
     1801 atgctgatta ccgatcagac tgccgtgggc gacaggctca gctatggcac gctgaaactg
     1861 gatgcgcagg cccacaacac cttactggat ggcgcgccgc tcgatctcag cccgaccgag
     1921 tttgcgctct tgcatctctt cttgcgcaat cctggtcgag cttttagccg ggcgtacctg
     1981 ctcgatactg tgtgggatga gacctacgtc gggggtgatc gatcggtaga caacaccgtt
     2041 ctgcggctac gcaaaaagct gggttcgctg ggggaagcta tcgagacggt ctggggtatt
     2101 ggttatcgac tgcgacctga aaggtaacga tgcaatgccc cgctcttccc tcattttcaa
     2161 tatgctgccg gtgcgcgcaa tccttgagac gcttctggcg ggactgacac tctggctcgt
     2221 cctgctgttg ttccgtcctc tgctagcgtc tctcatctgg agactcagtc tctgcctcgg
     2281 cattggtgta atatgcgtca tctttttcgc tttgcgattg ctgcggtcag caggttcccg
     2341 gcaacaaatc ctgctctacg aaaccgccct tggcggtgcc atctgcctta taatgagctg
     2401 cctggagctg ataacacttc ttattctgat ccagggagtg tcgcttagcc caccatggag
     2461 agacaccaac aggccgctat tgactgcctt gattgcgctc ctcctgaatc tcgtggtctt
     2521 ctttgtcacc cgctcggtaa tacgcctgtg gttcttctgg aatcaattgc gacgcagaca
     2581 gcttctgtgg gctttgacac atgcccatct gatgatcctc ctattggggg caggcttgct
     2641 gctggtggtg ctggagacgc tcgttctcct taactcaccc gatgtattcc tgatcgtgtc
     2701 gacgacactc ggtctgatcg tcttgagtat caccgggctg ctcctggtcc ttccactcct
     2761 ggcgctgttc tcgtatcttg ttatacgccg taccacacaa cgtctgcaaa cgctggctgc
     2821 cgcgacgagc gcgttgcggc aaggcaacta tgccatacgt gttccagttg gaggtgaaga
     2881 tgaagtagcc aggctccagg cagacttcaa tggcatggct gctgatcttg aacatgccat
     2941 gctggcgctt caggaagagc gcgatagagt tgcagctttg ctgcaagcgc gccgtgagat
     3001 gatcgccacc gtttctcacg agttacggac gcccgtagcg acactacgcg gctatcttga
     3061 aacgattctc ctacactggg aagagccgtc aaacctgaca cttcatcgcg acctggaagt
     3121 gatggaggag gaagtgttac acctccaatt cctcgtcgaa gagcttttta cgctggcgcg
     3181 aacggatgta ggcaggctaa cgctacattg tgagccgacc gatattggag cgatcgcgca
     3241 gcgcgttgtc gagaccatgg cgccgctcgc gtggcgaacg agcaagatcg agatgatcgc
     3301 ggaagcgcct gccgtgttgc ctcccgccct ggtgaacgcg aatcgcctgg aacaggttct
     3361 ccagaatttg ctgcacaatg ccgtgcgcca caccgcgcct ggaggcatcg tggctgtaat
     3421 ggtaacggaa gaacagacag ctattacgct ccaggtgaaa gacaccggtg agggtattgc
     3481 tgcggcagat ctaccccata tctgggaacg cttttaccag atagaggata cccggacacg
     3541 gacgaatggc ggtgttgggc ttggccttgc gctggtcaaa gagtggatcg aggaaatgag
     3601 cggaaccgta acggttgaga gcgtggttgg tgaaggtagt tgtttccata ttcgcctgcc
     3661 aaaagtcgtg gaggaggg
//
LOCUS       DASZAK010001190         1779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_492091, whole genome shotgun sequence.
ACCESSION   DASZAK010001190 DASZAK010000000
VERSION     DASZAK010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492091"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /gene="panC"
                     /locus_tag="VGT44_21015"
     CDS             <1..846
                     /gene="panC"
                     /locus_tag="VGT44_21015"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEV2583353.1"
                     /translation="SSKNVGFVPTMGYLHEGHLSLVRQGRKENEILTASIFVNPTQFG
                     PHEDLGSYPRDLPRDLQMLENLGVDIVFVPTTDEIYPPGFVTYVDPTGPLTMEAEGAS
                     RPGHFRGVATVVLKLFQIMQPHQAYFGQKDAQQVAVISRMVTDFDLPVKLRILSTIRE
                     ADGLAMSSRNAYLDPAERAASSVLYRALQTGRMVFEAHAKTRSREGVSEVVKAMTGTV
                     LKEPGAHLDYAEVRDPDSFMPLEATRAPALLLIAAKVGPARLIDNFLLRADGSWDTGI
                     PVNQQ"
     gene            873..1724
                     /gene="panB"
                     /locus_tag="VGT44_21020"
     CDS             873..1724
                     /gene="panB"
                     /locus_tag="VGT44_21020"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEV2583354.1"
                     /translation="MRDKVTVPAILARKGGSKLKVVTAYDTPSAHIADRAGADIILVG
                     DTLAHVVLGYEDTLPATIDVMIHHTAAVARAKPDALVLGDMPWLSYHISIEETVRNAG
                     RLIREGRAEAVKIEGGRKRLPMVEALLAAEIPVMGHLGLTPQSIHAMGGYRVQGRRAE
                     AARDLVEDAHALAATGVFAIVLEGVPDLVAQVVTQEVPVPTIGIGAGPNCDGQVLVYH
                     DVLGLHHDRLAKFVRQYAHLADLATEALEHFFADVETGAFPSDAESYHMDEQSARAFR
                     ALIRNEK"
BASE COUNT          419 a          483 c          503 g          374 t
ORIGIN      
        1 agctccaaaa atgtcggatt tgtccctact atgggatatc tccatgaagg gcatctttcg
       61 cttgtgcgcc agggacgcaa agagaacgaa atccttaccg cgagtatttt cgttaaccca
      121 acccagtttg gcccgcacga agacctggga agctacccgc gcgacctgcc acgcgatctg
      181 caaatgcttg agaatcttgg cgtagacatt gtcttcgttc caacaacgga tgagatttac
      241 cctcctggct tcgtgaccta tgtagaccca acagggccgc tcactatgga ggcagaggga
      301 gccagccgcc ccggacattt tcgcggtgtg gcgacggtag tgttaaaact cttccagatc
      361 atgcaacccc accaggcgta cttcggtcag aaggacgcgc agcaggtggc agttatctca
      421 cgcatggtga cagatttcga cctcccggtg aagttacgca tcctttcgac gattcgcgaa
      481 gctgatggcc tcgcaatgag cagcaggaat gcttatcttg acccggcgga acgcgcagct
      541 tcaagcgtgc tgtacagagc attgcaaact ggccgtatgg tgttcgaagc tcacgcgaaa
      601 actcgctcga gagaaggagt atctgaggta gtcaaagcga tgaccgggac agttctgaag
      661 gagcccggcg cgcaccttga ttatgcagag gttcgagacc ctgatagttt catgccatta
      721 gaggctacac gcgccccggc tttgctgctc atcgctgcaa aggtcggtcc agcccgtttg
      781 attgacaatt tcttattacg cgcggatggc agctgggata ctggcatccc tgtcaaccag
      841 caataaacga accaggtgaa aggaacaata ttatgcggga caaagtaact gtacctgcga
      901 tacttgctcg caagggtgga tcaaaactga aggtagtaac cgcctatgat accccgtcgg
      961 cgcacatcgc tgatagggca ggagcagata ttatcctggt aggcgatacg ctcgctcatg
     1021 tagtgctggg ctacgaagac acacttcctg ccacgataga tgtaatgatt caccatacgg
     1081 ctgctgttgc gcgcgcaaaa ccagacgcgc tggtgttagg cgatatgcca tggcttagct
     1141 atcacatttc tatagaagag accgtgcgca atgcgggccg tttaatacgt gagggccgcg
     1201 ccgaagcagt gaaaatagag ggcggacgga aacgattacc aatggttgaa gcactcctgg
     1261 ctgccgagat accggtgatg gggcaccttg gacttacccc gcaatcaatc catgccatgg
     1321 gtggctaccg ggtgcaaggt aggagggccg aggccgcgcg agacctggtt gaggatgcgc
     1381 atgcgctagc agcaacgggg gtcttcgcca tagttctgga aggcgtaccc gatttagtag
     1441 cgcaggtggt aacccaggag gtgccggtgc ccaccatcgg catcggcgcc ggccccaatt
     1501 gcgatgggca ggtgcttgtc tatcatgatg tattaggcct gcatcacgac cggctagcga
     1561 agttcgtccg ccaatacgca cacctggccg atctagctac cgaagccctt gagcacttct
     1621 tcgccgatgt cgagacagga gcttttccct cggacgccga gagttatcat atggacgagc
     1681 agtccgcgcg cgcttttcgc gccctgataa gaaatgagaa ataaacccat ccatcagggc
     1741 gtggcaagcc ccctcgctgg tctctcaagt ccgcgccta
//
LOCUS       DASZAK010001191         5483 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_102960, whole genome shotgun sequence.
ACCESSION   DASZAK010001191 DASZAK010000000
VERSION     DASZAK010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102960"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3154)
                     /locus_tag="VGT44_21025"
     CDS             complement(<1..3154)
                     /locus_tag="VGT44_21025"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HEV2583355.1"
                     /translation="MQALENKKPAPPAQEIFNRPPRIWPSFPEGKVIVPTPPRRENLP
                     PRPPGITLVMPVIMVGMMLCVYALVERSSPQQLVFLLPMAMFSIMTPITTVITSRQNI
                     RRVKRQWKKNEKQYRAILDGLRVELTEKLDEQRRLALLKDPDNTDLERYVKERAHLWE
                     RRPDDPDFLAVRVGSGTLPFAIEIQAPEIDTAEPMLPDVQQFQKEFTTVSGVPNCVSL
                     TKVKSLGLTGPRQIVADFARGLLCQIATLHSPEDVRIVGLFPTSQQQDWEWLKEIPHT
                     LPLKGSRHNKVERLFAAGQEEADQVLNLLLEELSQRATKEGEGQVSPVANNGVEEMPL
                     PHIVVIVHDYIEARKHPALSNAFKLGEQLGVSVIYLVAQQSAIPSECRGVVRILDADC
                     LTYAAAGYAGDTLRDVRPDIMEVAMARNIAKALAPLHIATEGEDAVDLPTNARLLDLL
                     GIPYADQFNVEQWWNLGGSGGQPPFGRLRVPMGAGQDGTVWIDFSDNAHGPHGIIAGT
                     TGAGKSELLSSLIIGLALTHHPHLVNFVLVDFKGGAAFKPFEKMPHTVGMVSDLSGNL
                     TERALHALKSELRRREHILGEANVKKIAQYQALHAQNPTTFPPLPNLFIIIDEFAELA
                     KEHPTFMDGLVSVVQKGRSLGVHLILATQKPTGSVNANIWSNLKFRICLRVASVQDSR
                     DILGRSEAGLLPISIPGRTYFQVGAEIFELFQSARISQPARVADESLLVAKPGGEEVT
                     DQRVLVSAMEQYTEEMQRELFLPWPDPLPRGVSLQELAQRHDLPVPIDKDMQDTPRYG
                     WLTFPTGLVDLPIEQRQEPLMLDLPRQGGHIVVAGASASGKSVFLRTLILSLAQTHTP
                     AQLHLYLIDYGGQALRVFEQLPHVGGVFGESHAEYIRRLLRKLDGIIEERKEICRTQQ
                     IDDFLAYQRRRLDNPSLPEMAAVVLVIDKFIEFKLAHEKELDSLLSIARSGRGYGVFL
                     VISADRPIAVPPQLMSLIDIRFGLRLIEPTDSLILLGKHDAAHLDPALPGRAYRRGKT
                     LDEVHFALPIMGDDDDEQTRM"
     gene            3585..4922
                     /gene="dnaB"
                     /locus_tag="VGT44_21030"
     CDS             3585..4922
                     /gene="dnaB"
                     /locus_tag="VGT44_21030"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV2583356.1"
                     /translation="MEKLLPQNIEAECGVLGSIIIDPEAIVQISDFLRSDDFYRDAHR
                     TIFEVIVQLYEEREPADFITICDELERRSQLEEVGGASYITSLINQVPTSGNVEYYGR
                     IVERSAILRRLIHAAGQIAAVAYESSDADAALEKAEQLIFAISQRHARSDFTHLREIL
                     ADYMDKLDQLHERRGSIVGVPTGFTDLDRLTGGLQKSDLIVLAARPGLGKTSLALSVA
                     HNAAAKYQRSVAVFSLEMSKEQLAQRLLSMDASIDQQRLRTGWIEDDEWERIVIARDK
                     LEEVNIWIDDTAGITTTELRSKARRLQSEYGIDLIIIDYLQLMQATIGGKRNENRVME
                     ISEISRSLKALARELNLPVLALAQLSRAVESRQSKVPQLSDLRESGSIEQDSDIVMFI
                     YRDDVYNQESEKKNIADVIIAKHRNGPLGEISLYFQANQTRFSDLEANPGMEE"
     gene            4938..>5483
                     /locus_tag="VGT44_21035"
     CDS             4938..>5483
                     /locus_tag="VGT44_21035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583357.1"
                     /translation="MTLFAGFPAGKNPYVPVPEVFFTVLLPEIEDMAELKVTLHLFWL
                     LAQKKGEPRCVSDRELIGDAVLLRSLKRKGDPRPVEERLQHGLEQAIARGTILRIYLK
                     LLNDISDEEELVGWYFFNTARSRKVVQELQGGEVLPASLLEAETEPVAQEQDAAVALG
                     AGGGSGRPTAYTRRETQAHVQV"
BASE COUNT         1135 a         1582 c         1664 g         1102 t
ORIGIN      
        1 acatgcgtgt ctgttcatca tcatcatcac ccatgatcgg cagggcaaaa tgcacttcgt
       61 cgagcgtttt gccacgccgg taggcgcgtc ctggcagcgc cggatcgaga tgcgccgcgt
      121 cgtgcttgcc gagcaggatc agcgagtccg taggttctat cagccgcagg ccaaagcgaa
      181 tatcgatcag gctcatcaac tgcgggggta ctgctatagg acgatcggcg ctgatgacca
      241 ggaagacgcc gtagccgcgt ccagaacgcg cgatggagag cagcgagtcc agttctttct
      301 cgtgcgccag cttgaactcg atgaatttgt cgatgaccag cacgacggcg gccatttcgg
      361 gcagcgatgg attgtcgagg cggcggcgct ggtaagccag gaaatcgtcg atctgctggg
      421 tgcggcaaat ctccttgcgc tcctcgataa tgccatccag tttgcgcagc aggcggcgga
      481 tgtattctgc gtgcgattcg ccgaatacgc cgccgacgtg cggcaattgc tcaaagacgc
      541 gcagcgcctg gccgccatag tcgatcaggt acagatgcag ttgagcgggc gtgtgcgttt
      601 gcgccagtga gaggatcagc gtgcgcagga acacgctttt gcccgaagcg gacgcgcccg
      661 cgaccacgat atgaccgccc tggcggggca ggtcgagcat gagcggttcc tgcctctgtt
      721 caatcggcaa gtcaaccagg cccgttggga acgtcagcca gccatatctg ggcgtatcct
      781 gcatatcctt atctatgggc acgggcaggt cgtggcgctg cgccagttct tgcaggctga
      841 cccctctggg cagcggatcg ggccagggca ggaacagctc gcgctgcatc tcctccgtat
      901 attgctccat tgcgctgacc agcacacgct ggtcggtgac ctcttccccg cccggcttgg
      961 cgaccaggag ggactcatcc gccacgcgcg cgggctgcga gatgcgcgcg ctctggaaca
     1021 gctcgaagat ttccgcgccg acctggaagt aggtgcgccc cggtatgctg atgggcagca
     1081 agcccgcctc ggagcggccc agtatgtcgc ggctatcctg caccgaggcg acgcgcaggc
     1141 agatacggaa cttgaggttt gaccagatgt tggcgttgac cgagccggtc ggtttctgcg
     1201 ttgccaggat cagatgcacg cccaggctgc ggcccttttg caccacgctc accagcccgt
     1261 ccataaaggt gggatgctct ttggccagtt ccgcgaactc gtcgatgatg ataaacaggt
     1321 ttggcagcgg cggaaaggtg gttgggtttt gggcgtgcag cgcctggtac tgcgcgattt
     1381 tcttgacatt ggcctcgccc agaatatgct cgcgccgcct gagctcgctt ttgagcgcgt
     1441 gcagggcgcg ttcggtcagg ttgccgctca ggtcgctgac catgccgact gtgtgcggca
     1501 tcttctcgaa gggcttgaag gccgcgccgc ctttgaaatc taccagcacg aagttgacca
     1561 ggtgtgggtg gtgcgtcaat gccaggccga tgatgagcga agagagcagc tcgcttttgc
     1621 ccgcgccggt ggttcccgcg atgataccgt gcgggccgtg cgcgttatcg ctaaaatcga
     1681 tccagactgt cccgtcctgc ccggcgccca tcgggacgcg caggcggcca aaaggcggtt
     1741 gcccgccgct tcctcccagg ttccaccact gttcgacgtt gaactgatcg gcataaggaa
     1801 tacccagcag gtcgagcagg cgtgcgttgg taggaagatc gacggcatcc tcgccctcgg
     1861 tggcgatatg cagcggagcg agcgctttgg cgatgttgcg cgccatggcg acctccatga
     1921 tgtcggggcg cacgtcgcgc agcgtgtcgc cggcataacc tgccgcagca taggtcagac
     1981 aatcggcgtc caggatgcgc accacgcctc gacattcgct ggggatggcc gactgctgtg
     2041 cgaccaggta gatcaccgag acgcccaatt gctcgcccag cttaaaggca ttgctcaagg
     2101 ccggatgttt gcgcgcctcg atgtagtcat gcacgatgac gacgatatgc ggcagcggca
     2161 tctcttccac accgttattc gccaccggag agacctgacc ctcgccctcc ttcgttgcgc
     2221 gctggctcaa ctcctccagc agtaaattga gtacctggtc ggcttcctcc tggccagcgg
     2281 caaagagccg ctcaaccttg ttatgcctgc tccccttcaa aggcagtgtg tgcggaatct
     2341 ccttgagcca ttcccagtcc tgctgctgcg aggttgggaa caggccaacg atgcgcacgt
     2401 cctccggtga atgcaatgta gcgatctggc agagcaaacc acgagcgaag tcggccacga
     2461 tctggcgggg gcccgtcagg cccaacgact ttactttggt gagagaaacg cagtttggca
     2521 cgccgctgac ggtggtaaac tctttctgga actgctgcac gtctggcagc atcggttcag
     2581 cagtgtcgat ctccggcgct tgaatttcga tggcgaaagg cagtgtgcca cttcctacac
     2641 gcaccgccag aaagtcgggg tcgtcgggcc gccgctccca gagatgcgcg cgctctttga
     2701 catatcgctc caggtcggta ttgtcggggt ctttgagcag cgccaggcgg cgctgctcgt
     2761 caagcttttc ggtcagttcc acgcgcagcc catctaaaat cgcccggtat tgcttttcgt
     2821 ttttcttcca ctgccgcttc acgcgccgaa tattctgcct ggatgtgata accgtcgtaa
     2881 taggcgtcat aatcgagaac atggccatcg gcagcaggaa tacgagttgc tgcggcgaac
     2941 ttcgctcaac cagcgcgtac acgcacagca tcatacccac cataatcacc ggcatcacca
     3001 acgtaatgcc aggcggcctg ggagggagat tctcgcgcct gggcggagtg ggtacgatga
     3061 ccttcccctc cgggaacgaa ggccagatac gcggcgggcg gttaaagatc tcctgcgcag
     3121 gcggtgccgg ttttttgttc tcaagcgctt gcatcgtctc tgctccatgt gtgctaagca
     3181 ggtcttgctg gttcgaccaa atcgtgactc aggctaaaca atcgtcgacc cggcaggtcg
     3241 atgccagtgc tgaaagtccc aactacaacg aatttctgtg gatatccatg tggataaggt
     3301 tggggataag cggggataac ctggtcattt gcgtggataa ccgggggaaa accatactta
     3361 tgtcacgctg ctcgcacaag ccacgcacga gagaaatcag acatatccac atatccacag
     3421 agcatatcgc ctgcttgctg attgatttac cgccgaagat tcgctacact agtgaggcac
     3481 ccaatttcaa ttgccagttt gccctgggaa taatcgaact tatgtgctat aatcggcctt
     3541 agaaggcaac tctatttaca tcgaagcagg ggacatcgaa catcgtggag aagctcttac
     3601 cgcaaaatat agaagcagaa tgtggtgtgc taggcagcat cattattgat ccagaagcga
     3661 tcgtgcaaat ttccgatttt ctgcgttcgg acgactttta tcgcgacgcg caccgcacca
     3721 tcttcgaggt gatcgtgcaa ctctacgagg agcgcgaacc ggcagacttc atcaccatct
     3781 gtgatgagct ggagcggcgc agccagttgg aagaggtcgg cggagccagc tatattacct
     3841 cgctcatcaa ccaggttccc accagcggca atgtggagta ttatgggcgt atcgtcgaac
     3901 gttccgccat cctgcgccgc ctgatccatg ctgccgggca gatcgccgcc gttgcctatg
     3961 aatccagcga tgccgatgcc gcgctagaaa aagctgagca gctgattttc gccatcagcc
     4021 agcgtcacgc tcgctccgac ttcacccacc tgcgcgaaat cctggcggac tacatggaca
     4081 agctcgacca gttgcacgag cggcgcggta gcatcgtggg cgtccccacc ggtttcaccg
     4141 acctggaccg gctgaccggc ggcctgcaaa aatcagacct gatcgtgctg gctgctcgtc
     4201 ctggattagg caaaacgtcg ctggcgctga gcgtagcgca caacgccgcc gccaaatacc
     4261 agcgttcggt ggcagtcttc agtctggaga tgagcaaaga gcaactggca cagcgtctgc
     4321 tctcgatgga cgcctccatc gatcagcagc gcctgcgcac cggctggatc gaggacgacg
     4381 agtgggagcg catcgtgatc gccagggata agctggaaga ggtgaacatc tggatcgacg
     4441 ataccgccgg tatcaccacc accgaactgc gcagcaaagc ccgccgcttg caatccgagt
     4501 atggcatcga tctgattatc atagactatc tccagttgat gcaagccacc atcggcggca
     4561 aacgcaacga gaaccgtgtc atggaaatct cggagatcag ccgcagcctg aaagcgctcg
     4621 cccgcgaact caacctgccg gtgttggccc tggcgcagct ctcgcgcgcg gtggagagcc
     4681 ggcagtcgaa ggtgccgcag ttgtcggacc tacgcgaaag cggaagtata gagcaggata
     4741 gtgatatcgt catgtttatc taccgtgacg atgtgtataa ccaggagtcg gagaagaaga
     4801 acatcgcgga cgtgatcatt gccaagcacc gcaacgggcc gcttggcgaa atcagcctgt
     4861 actttcaggc caaccagaca cgcttctctg acctggaagc aaacccgggg atggaagaat
     4921 aaaaggactg tatttgtatg acactctttg ccggatttcc agcggggaaa aatccgtatg
     4981 tgccggtacc ggaagtattt tttacggtac tgctgcccga gatcgaggac atggcggaac
     5041 tcaaggtgac gctgcacctc ttctggctgc tggcgcaaaa gaagggcgag ccgcgctgcg
     5101 tcagcgaccg cgagctgata ggcgatgccg tgctgctgcg cagtctcaag cgcaaggggg
     5161 acccgcgacc ggtggaagaa cgcctgcagc atgggttgga acaggccatc gcgcgcggca
     5221 ccattttacg tatttatctc aagctgttga atgatatcag tgatgaagag gaactggtgg
     5281 gatggtattt ctttaatacc gctcgcagcc gaaaagttgt gcaggagttg cagggaggcg
     5341 aggtgctgcc ggccagcctg ctcgaggcgg agacggaacc ggtggcgcaa gaacaggacg
     5401 cggctgtggc gttgggagca ggtgggggaa gtgggcggcc aacggcgtac acacgccgcg
     5461 agacccaggc gcatgtgcag gtg
//
LOCUS       DASZAK010001192         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_30652, whole genome shotgun sequence.
ACCESSION   DASZAK010001192 DASZAK010000000
VERSION     DASZAK010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30652"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="VGT44_21040"
     CDS             complement(<1..1309)
                     /locus_tag="VGT44_21040"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV2583358.1"
                     /translation="MTQTAQPLMVGTSLPRQDGPDKITGRARYAGDQTVPGMLYAYLV
                     TSPYAHARILNIDTSAAVSLPGVVAAFTAETLGMAQAESLSRSQSPLAVQEALWCGHP
                     VAIVVGETEASAEDGAAAVEVDYEPLPAAIDPEAAMRLDSPLARLQSEIPATTGGGAH
                     AAIPREGAAFEQEEHSQNVSQARPLRMGDIEAGLREADVVVESSYRTHPVHQSYMEPH
                     SVTVAPSLSGHHLVIWPSTQGMFNVRSAVASALKMPERQIRVESVPIGGGFGGKETLL
                     EPLAAAVASRLRKPVRLVYTRQEDLLAGNPAPQMAITVKLGAKRDGTLTAIQVRMILD
                     AGAYPNPLAGFSGFHFAGIYRCPNLDIRCDIVQTNKPGTGAYRAPSGPQAYFALESTV
                     QDLCQQLGMDPLRFRQINALKGGDPSMMLGRWPRIGLLECLEQI"
     gene            1511..>2003
                     /locus_tag="VGT44_21045"
     CDS             1511..>2003
                     /locus_tag="VGT44_21045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEV2583359.1"
                     /translation="MRKLRYCLVDVFTDRAFGGNPLAVFTDADGISPVMMQALAKELN
                     LSETTFVLPARAATHDYSVRIFTPAVELPMAGHPTIGTAFVLAHEHSIQAHETETTVL
                     FEEGVGIIPVTLTFQDDHIQSIQMSQPLPTFGSQFVNREAIAQMLSLDTAALDRTLPL
                     EVVS"
BASE COUNT          427 a          570 c          551 g          455 t
ORIGIN      
        1 ggatctgctc gaggcactcc agcaggccga tccgtggcca ccggcccagc atcatagagg
       61 ggtcaccacc tttgagggca ttgatctggc ggaaccggag gggatccatc ccaagctgct
      121 ggcagaggtc ctgtaccgtc gattccagcg cgaagtaggc ttgcgggcct gaaggggcgc
      181 gataagcgcc ggtgccgggc ttgttggtct gaactatatc gcaacgaatg tccaggttgg
      241 gacagcgata gataccggcg aaatggaagc cgctaaagcc ggcaagagga tttgggtagg
      301 ctccagcatc aagaatcatc ctgacctgga tagcggtgag cgtgccatcc ctcttcgcac
      361 cgagtttgac agtaatcgcc atttgcggcg cggggttccc tgctagaaga tcttcctgtc
      421 gcgtgtagac caatcgtacc ggcttgcgca ggcgtgaggc cactgctgct gccagcggct
      481 ctagcagggt ctccttcccg ccaaaccctc caccgattgg cactgattcg acacggattt
      541 gtcgctctgg catctttaag gcacttgcga ctgccgagcg cacattgaac atcccctgtg
      601 tgctgggcca gatcaccagg tgatgaccgg aaagactggg cgctactgta accgaatgtg
      661 gttccatata ggactggtgt accgggtggg tgcgatagct gctttcgacc accacatcag
      721 cctcgcgcag accagcctcg atatcaccca tccttagagg ccgcgcctgg ctgacatttt
      781 gcgaatgttc ctcttgctcg aacgctgccc cctctcttgg gatggcggcg tgtgcgcccc
      841 ctccggtggt tgccgggatc tcactctgca agcgagccag gggcgaatcc agccgcatag
      901 cagcctcggg atcgatggca gctggtagag gctcgtaatc gacttccacg gcggcggctc
      961 catcctctgc gctagcctcc gtctcaccga ccacgatggc tacaggatga ccgcaccaca
     1021 acgcttcttg cactgccagt ggggattgcg aacgagagag cgattctgcc tgggccattc
     1081 ccagcgtttc ggcggtaaaa gccgctacga ctccaggcag cgacacagca gccgacgtat
     1141 caatgttaag gatacgtgca tgagcatatg ggcttgtgac caggtatgca tacagcatac
     1201 cgggcaccgt ctgatctcca gcataacgcg cacgcccggt tattttgtca ggtccatcct
     1261 ggcgtgggag agatgtgcca accataagag gttgcgctgt ttgcgtcata gggaaaatat
     1321 ctccttctct gtaacagaca cagttctgta cagtattcta tctcatcaaa ctgaatagtg
     1381 tgtgggtgac cgaggtgtag acgattggat agctgagtcc tcgtacctgc gacaggtgat
     1441 agcgcgagtt gaaacggttg atgtgataca ctcttcattg agccatcttg taccattgaa
     1501 tggagattgt atgcgaaaac tgcgctattg cctggtcgat gtctttacag atcgagcctt
     1561 tggcggcaat ccgctcgctg tatttacaga tgctgacggc atttcccccg tgatgatgca
     1621 agcactcgcc aaagaactga atctctcgga aacaactttc gtacttccgg cgcgtgccgc
     1681 cacacatgat tactctgtgc gcatttttac ccccgctgtt gagctgccga tggcgggcca
     1741 cccgaccatc ggcaccgcct ttgtcctcgc tcatgagcat tcgatacagg cccacgaaac
     1801 tgaaaccact gtcttatttg aagaaggggt aggtatcatt cccgtgacac ttacatttca
     1861 ggatgaccat atccaatcga tccagatgag ccaaccgctt cctacctttg gttcgcagtt
     1921 cgttaatcga gaggcaatag cgcaaatgct atcgcttgac acagccgctc tcgatagaac
     1981 gctgccgctc gaagtagttt cat
//
LOCUS       DASZAK010001193         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_172509, whole genome shotgun sequence.
ACCESSION   DASZAK010001193 DASZAK010000000
VERSION     DASZAK010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172509"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..421
                     /locus_tag="VGT44_21050"
     CDS             266..421
                     /locus_tag="VGT44_21050"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2583360.1"
                     /translation="MFVAQVGETVAGLTEIYLLQDEAHPLIVTYRYGYLQSLVVSESF
                     RKHGIWR"
     gene            539..1138
                     /locus_tag="VGT44_21055"
     CDS             539..1138
                     /locus_tag="VGT44_21055"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEV2583361.1"
                     /translation="MLGKSHLLVGITGGVVLDSLLHVSGPPLTGASHITLELLVNKCI
                     FYAMVAFGALLPDIDNARSTYGRKLGWVSREIQHVAGHRTLFHSLLGLLLGSVLAVGL
                     QQLVIYLLSQRGLTPPALYIGESQLVLVAVLLGCILHIAADALTEGGVPLFWPSHKRY
                     GFPPNPHWRFRTGDWPEPVIVFTFMFLVGLGIYYSVIIV"
     gene            1154..>2500
                     /locus_tag="VGT44_21060"
     CDS             1154..>2500
                     /locus_tag="VGT44_21060"
                     /inference="COORDINATES: protein motif:HMM:NF021379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2079 domain-containing protein"
                     /protein_id="HEV2583362.1"
                     /translation="MIKRVEGIKKLFNLRSQLWLAWAFLALVTIIYSLEMSHQAILRY
                     DTFKATAFDLGNMDQVLWNTIHGRLFQFTNQAIDWYGPPTRLAIHFEPIILPLSLLYA
                     FHADPRILLVFQTLALASGTVPVFLLTRKLIPAWPLLAALMATAYLLSPALLGLNIFD
                     FHPVALATPLLLFALLALEHRRVAWFVILCVLAACTKEDVPLAIALLGILVMWKYKLP
                     RLGLALLIGGALWSLLAFKVIIPHFYPGLQASNFWYRYEELGSSPGQAILNLFVKPWL
                     FFGLFFTMDRVYYLASLLRSTGFLPLLAPEWLLPTLPSLAVNLLSTDPLLYSGVYHYN
                     AAIIPFIMMAAIMGTRRLLSQWQAWREEDTIPDTPDAPVVAAAHASPWPLFASITAFA
                     RRRLQKLSNLRFLAAVKDRFARLALPARRDRQWARFSMRMRPLARSLPTFRLQWVLF"
BASE COUNT          434 a          783 c          667 g          616 t
ORIGIN      
        1 gcaaaggtac cgctgggaat tccacgaatg gcgccatgca caatatcctt gcgatctgtc
       61 tctccctcat ttatgagggt cagagttagc gcctcatcca tacgcttctc ctcctggctc
      121 aacatgatta gtacacagta gctttttcag ccctcagcag atgcgtacac atgtactatt
      181 ctgtttatat cataaacgcg cggcaagacg ctaatcccca tcactcataa ccaattctgg
      241 ctatcacgct tccagatgac ccgcaatctt tgttgcgcag gttggcgaaa ctgtggcggg
      301 gctgaccgag atctatctgc tccaggatga agcccatccc ctcattgtta cctatcgcta
      361 cggctacctg caaagcctgg ttgtctctga gtcctttcgc aagcatggca tatggcggtg
      421 aaccttaaat gaacattgac atgtgttcta aaacccccta caatgcggga aatgatgtac
      481 tgagaggcgc acccttgcgg tcgccctccc agagtacagg tgaggagttc tttcgcgcat
      541 gttagggaag tcgcatttac tggtcgggat caccggcggc gttgtgctgg atagcctctt
      601 acacgtatcg gggccgccgc tgacgggcgc gtcgcatatc acgttagagc tgctggtgaa
      661 caagtgcatc ttttatgcca tggtcgcttt cggcgcgctt ctgccggata tcgataacgc
      721 gcgcagcacc tatggccgca agctgggctg ggtcagcaga gaaattcagc acgtggcggg
      781 ccatcgtacc ctctttcata gcttgctggg gctgttgctg gggtcagtgc tggcggtcgg
      841 cctgcaacaa ctggtgattt atttgctgag ccagcgcggc ctgacgccgc ccgccctata
      901 tatcggggag tcgcagctgg tgctggtcgc ggtgctgctc ggctgcatcc tgcatatcgc
      961 cgccgatgcg ctgaccgagg ggggcgtgcc actgttctgg cccagccaca aacgctatgg
     1021 gtttccgccc aatccgcact ggcgttttcg taccggtgac tggccagagc cggtaatcgt
     1081 cttcacattt atgttcctgg tcgggcttgg catttactat tccgtgatta ttgtatgagg
     1141 catgtcgctt ctcgtgataa agcgtgtcga aggcatcaaa aaattattta acctgcgcag
     1201 ccagctctgg ctggcctggg cgtttctggc tctggtgaca atcatctata gcctggagat
     1261 gagtcaccag gccatcctgc gctatgacac cttcaaggcc accgccttcg atctcggcaa
     1321 tatggaccag gtactgtgga acaccattca tggccgcctc ttccagttca ccaaccaggc
     1381 catcgactgg tacgggccgc caacgcgcct ggcaatccat ttcgagccga tcattttgcc
     1441 actctcgctg ctctatgcct ttcacgccga tccgcgcatc ctgctggtct tccagacgct
     1501 cgcgcttgcc tccggcacag tgccggtctt cctgctgacg cgcaagctca tccctgcatg
     1561 gccgctgctt gccgcgttga tggcgacggc ctacctgctc tcgcccgcgc tgctgggctt
     1621 gaatatcttc gacttccatc ccgtcgcgct cgccacgccc ctgctgctct tcgcgctgct
     1681 ggcattggaa catcgccgcg tggcctggtt cgtgatcctg tgcgtgctgg cagcctgtac
     1741 caaagaggat gtgccgctgg ccatcgccct gcttggcata ctggtcatgt ggaaatataa
     1801 actgcctcgc ctcggcctcg cgctgctgat cggcggcgcg ctgtggagct tgctggcatt
     1861 caaggtcatt atcccgcatt tctaccccgg cttgcaggcc agcaacttct ggtatcgcta
     1921 cgaggaactg ggcagttcgc cggggcaggc tatcctcaac ctcttcgtca agccctggct
     1981 cttctttggc ctcttcttca cgatggatcg cgtctactac ctggccagtt tgctgcgcag
     2041 tacgggcttc ctgcccctgc tggcccccga atggctcctg cccaccttac ccagcctggc
     2101 tgtcaacctg ctgagtaccg atcccctgct gtacagcggc gtctatcact acaatgccgc
     2161 tatcatcccc ttcatcatga tggccgccat catgggtacg cggcggctgc tctcgcaatg
     2221 gcaggcatgg cgcgaagagg ataccatccc tgatacccct gatgccccgg tggttgccgc
     2281 agctcatgcg tccccgtggc ctctctttgc atccataaca gcatttgccc ggcgcagact
     2341 gcaaaagctt tccaatctgc gcttccttgc cgctgtgaaa gatcgtttcg cgcgcctggc
     2401 gcttccggca aggcgggaca ggcagtgggc gcgcttcagc atgcgtatgc gacccctggc
     2461 gcgttcgctc cccactttcc gtttgcagtg ggtcctattt
//
LOCUS       DASZAK010001194         4042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_670851, whole genome shotgun sequence.
ACCESSION   DASZAK010001194 DASZAK010000000
VERSION     DASZAK010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670851"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1361
                     /locus_tag="VGT44_21065"
     CDS             <1..1361
                     /locus_tag="VGT44_21065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583363.1"
                     /translation="VTARQWSKLMDANRVRHGAHDTGAADERQYIRLRYSTQFTTGGR
                     AHTIEMEVPVPLGASPELRDQLIREAEANMEQLYRRVEARARGQRPSESTQRPAPPSQ
                     QPTQPAPSQQPQPYQPAQPARSTSPDQRTQPPQSAPAASPAPAAASVRESPSAISLTD
                     RRAGASSIPSAASASSAASAQQSQARPELPSVPGLASMGGGTMKLSEFIQIIRENWGL
                     APKEAMELLQVKSLNNMNYRDLLRQLETLVAQASESSASLNNRPASSDTPAQPGSFSP
                     ARPPMPAPSPAPRPATPRREQAPSSRPSAPSSPSSLPSRMEGPANIPVYPLRENIVRE
                     PAGVYKFDEEEEDDDEEMEIEVQEEGSMSEAMARIKIDELRDMRGTVAPNPGRLVVLH
                     NLLDSQISDDQFLQLIERLWSAVSDKKLKQDQVEGLISWAKEDYFEDEVRAVLSVLNK
                     QA"
     gene            1376..1942
                     /gene="rsmD"
                     /locus_tag="VGT44_21070"
     CDS             1376..1942
                     /gene="rsmD"
                     /locus_tag="VGT44_21070"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV2583364.1"
                     /translation="MRVVTGEAKGRRLKAPKTSGTRPIIDRVKTALFDILSTRVEDAR
                     FLDLFAGTGSVGIEALSRGASHATFIEMSHSVLKLVRENLKITGLGDRAETLHADAFK
                     FLQANATSAATREYDIIYVAPPQYHELAARALALLDTSPLVAPGGLVIVQVHPKERPI
                     VTAVPLTRLSLTDERKYGSTLLLFYEGV"
     gene            1946..2329
                     /locus_tag="VGT44_21075"
     CDS             1946..2329
                     /locus_tag="VGT44_21075"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2583365.1"
                     /translation="MPIQFQKLDYIIVYVSDMQRSVAFYRDILGLPMKFTSPGWTEFI
                     VGSTTLALHGAGTAPSPAEQVALPPSGQAQLGFMVDDLQATYEALVAQDIHFSLPPKK
                     QNSGVLLAVFHDPDGLGITIQQRQS"
     gene            2340..2825
                     /locus_tag="VGT44_21080"
     CDS             2340..2825
                     /locus_tag="VGT44_21080"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2583366.1"
                     /translation="MLNKPGSARFAFVDAGEAARMLGIDRVTLEQWVRDGRLKPHRGV
                     GRDSFYRTADLQALYNELHPATELAEAIAADERDSAGEQDSQSEAKAPARKKQDPQMR
                     VYLRLQADAKWFDTSEDDIQTWFNQLAPDGYERNKRNAEHTIRKLQFLVGLIEEAQGR
                     E"
     gene            complement(3167..>4042)
                     /locus_tag="VGT44_21085"
     CDS             complement(3167..>4042)
                     /locus_tag="VGT44_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583367.1"
                     /translation="FHATDIATAEYVAGVIHGLDWGQGEGRHLLTHDDVIAFKQAGGY
                     NNDWDMCYLLATLCTARLREWRGTALAERSFAEWAELAYQATQAGHGGMEWVRAVMPA
                     SAQLDYGVIGQIYHEFYWGADELRKRYGREARFLPDFYGFVHNEEMLFPPDFFRELRG
                     MGIAHLGIITGRVGPEVDMALEMMEAHAGERWWEVVVSADSAPKPDPRALQLAIAGIP
                     GGVGGGLFIGDTGDDLDVVKRYRAVKQVGEPEILAVMLVYEHEVEVYKERGADFIVRT
                     VTDVVGCLPRATRND"
BASE COUNT          906 a         1444 c         1023 g          669 t
ORIGIN      
        1 ttgtcacagc aaggcagtgg agcaagctta tggatgcgaa tagagtaagg cacggcgcgc
       61 acgacaccgg cgcagccgat gagaggcagt acatccggct gcgctatagc acgcaattta
      121 ccacgggcgg gcgcgcgcac accattgaga tggaagtgcc ggtgccgtta ggcgcaagcc
      181 cggagctgcg cgatcaactg atccgcgaag ccgaggccaa catggagcag ctctatcgcc
      241 gcgttgaagc gcgcgcgcgc ggccagcgcc cgtccgaatc cacccagcga cccgcgccgc
      301 catcccagca gccaacgcag cccgctcctt cccaacagcc tcaaccatat caaccggcgc
      361 agccagcccg ctcaacatcg cccgaccagc gcactcagcc gccgcaatcc gctcccgccg
      421 catcaccagc tcctgccgcc gcatcggtgc gcgaaagtcc atctgccatt tctttgaccg
      481 atagacgcgc aggagcctcg tctatcccgt ctgccgcatc tgcctcatca gccgcatctg
      541 cgcagcagtc ccaggcgcgc ccggaactac ccagcgtacc cggcctcgcc agcatgggcg
      601 gtggcacgat gaagctcagc gaattcatcc agattatccg ggagaactgg ggattagcgc
      661 ccaaagaagc catggagcta ttgcaggtca aatcactcaa taatatgaac tatcgcgacc
      721 tgctacggca actcgagaca ctcgtcgcgc aagcatcgga aagctcagca agcctgaaca
      781 accggcctgc ctcgtccgac acacccgcac agcctggctc attcagcccg gcgcgccctc
      841 ccatgcccgc accatcgccg gcaccgcggc ctgccacgcc tcgccgcgag caagcgccat
      901 cgtcacggcc ttccgcgcct tcatcaccct catcgctacc gtcgcgcatg gaaggtcccg
      961 cgaatatccc ggtctacccc ttgcgcgaga atatagtgcg cgagccagcc ggcgtctaca
     1021 agttcgacga ggaagaagag gacgacgacg aagaaatgga aatcgaagtg caggaagagg
     1081 gcagcatgtc ggaggccatg gcacgcatca aaatcgacga gctgcgcgac atgcgcggca
     1141 cggtcgcccc caatcctggc cgcctggtcg tgctgcacaa cctgctcgac tcgcagatca
     1201 gcgacgacca gttcctgcaa ctgatcgagc ggctctggag cgccgtatca gataagaaac
     1261 tcaaacagga ccaggtagaa ggcttgatct cctgggcgaa agaagactat ttcgaggacg
     1321 aagtccgagc cgtgctatcc gtcctcaaca aacaagcata gaaagtaatt gaaatatgcg
     1381 tgtagttacc ggcgaagcca aagggcgcag gctcaaagcc cccaaaacat cagggacgcg
     1441 ccccatcatc gaccgcgtca agaccgccct cttcgacatt ctttcgacgc gcgtagagga
     1501 tgcgcgtttc ctcgacctct tcgccggcac cggcagcgtc ggcatcgagg cgctgagccg
     1561 cggcgcatca cacgccacct tcatcgaaat gagccacagc gtcctgaagc tggtgcgcga
     1621 aaacctcaaa atcaccggac ttggcgaccg cgccgaaacc ctccacgccg acgccttcaa
     1681 attcttgcaa gccaatgcta ccagcgcagc cacgcgcgag tatgatatca tctacgtagc
     1741 gccgccgcag tatcacgagc tggccgcgcg cgccctggcc ctgctggaca cctccccgct
     1801 cgtcgcaccc ggcggcctgg taatcgtcca ggtacaccca aaagagcgcc caatcgtcac
     1861 cgccgtcccg ctcacccgcc tatccctgac cgacgagcgc aaatacggga gcacattgtt
     1921 actgttctac gaaggagtat aaataatgcc catccaattc caaaaactcg actatatcat
     1981 cgtctacgtc agcgacatgc agcgctccgt cgccttctat cgcgacatac tcggcctgcc
     2041 catgaaattc acctcgcctg gctggacaga atttatcgtg ggcagtacga ccctggctct
     2101 gcacggcgca ggcaccgcgc catcgccggc agagcaggtg gcgctgccgc cctccggcca
     2161 ggcacagctt ggctttatgg tagacgacct gcaggccacc tacgaagcgc tggtcgcgca
     2221 ggacatccac ttctcgctgc cacccaaaaa gcagaactcc ggcgtgttgc tggccgtctt
     2281 ccacgacccc gatggcctgg gcatcacgat ccagcagcgt cagagctagg agtacactca
     2341 tgctaaacaa acccggcagc gcgcgtttcg cctttgtcga cgccggcgaa gccgcccgaa
     2401 tgctcggcat cgaccgtgtc acgctggagc agtgggtgcg cgatggccgt ctcaaaccgc
     2461 atcgaggcgt gggtcgcgac tcgttctacc gcaccgccga cctccaggcg ctctacaacg
     2521 aactgcaccc cgccaccgaa ctggccgaag ccatcgcagc cgacgaacgc gacagcgcag
     2581 gcgaacagga cagccaatcc gaagcaaaag cgcccgcgcg caaaaagcaa gacccccaga
     2641 tgcgcgtcta cctgcgcctg caagccgacg ccaagtggtt cgacacctcc gaagacgaca
     2701 tccaaacctg gttcaaccag ctagcccccg acggctacga acgcaacaaa aggaacgccg
     2761 aacacaccat caggaagttg cagtttttgg tggggttgat tgaggaggcg caaggacgag
     2821 aatagaggca ctttcaaccc accgtaggag ccggtttacc gcgctcaaag ccgatttatc
     2881 ggcgcgaatc ctcgtcgatc cggtcaatgg aaccgaccga taaggcatac cgacaaatta
     2941 atacggacat ctgtgctgta aatcccaggg cgaccgcaat ggggtggaga cccttgcggt
     3001 cgccctcgta taccctgaac aggttcagcc ctttcactcc caacgcatct ccctttccac
     3061 atcttcccag gctatacatt tcccctcatt catctcctga accccttgcc tgtacgaggc
     3121 catcagttcc ttatcctgta caatttccag cgtctcctcc aacgattcaa tcgtttcgag
     3181 tagctcgcgg caaacacccc accacatccg tcaccgttcg cacaataaaa tccgcgccgc
     3241 gctccttata cacctctacc tcatgttcat aaaccagcat caccgcgagt atctccggct
     3301 cgcctacttg tttgacagcg cgatagcgct tcaccacgtc caggtcatcg cccgtatcgc
     3361 cgataaacag cccgccgccg acaccgccag ggatgcccgc gatggccagt tgcagcgcgc
     3421 gcgggtccgg cttgggcgcg ctatccgctg agacgaccac ctcccaccag cgctcgcccg
     3481 cgtgcgcctc catcatctcc agcgccatat ccacctccgg cccgacgcgc ccggtaataa
     3541 taccgagatg tgcgatgccc atgccgcgca gctctcggaa gaaatcaggc ggaaacagca
     3601 tctcctcgtt atgcacaaag ccatagaaat ccggcaggaa gcgcgcctcg cggccatagc
     3661 gtttgcgcag ttcgtccgcg ccccagtaaa actcgtgata aatctgtcca atcaccccat
     3721 aatcaagctg cgccgacgcg ggcatcaccg cgcgcaccca ctccatgccg ccatgtcccg
     3781 cctgcgtcgc ctggtacgcc agctcagccc actccgcgaa gctcctctca gccagcgccg
     3841 tccccctcca ctcgcgcagc cgcgccgtgc agagcgtcgc cagcaaatag cacatatccc
     3901 aatcattgtt atagcccccc gcctgcttga acgcgatcac atcatcatgc gtcagcagat
     3961 gcctcccctc gccctgtccc caatccagcc catgtatcac ccccgccaca tattccgccg
     4021 tcgcaatatc cgtcgcgtga aa
//
LOCUS       DASZAK010001195         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_206338, whole genome shotgun sequence.
ACCESSION   DASZAK010001195 DASZAK010000000
VERSION     DASZAK010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206338"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VGT44_21090"
     CDS             complement(<1..672)
                     /locus_tag="VGT44_21090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583368.1"
                     /translation="MQRNRRERTSQIQQRQQTVVATDKANSEVALGQIDQAFTDRQSG
                     VDEFEQVTTTGDGDVATIRLLLVDDHVLVREGLRRLLELENDIVVIAGAANGQEALQA
                     AHSLRPDVVLMDISMPAVDGIAVTRQMVEELPSVAVIMLTVFRQQQHILRAMRSGARG
                     YLLKSATLEEVAQAIRKVHEGGTFISSELTGELVNEYRRLSDATATSRPSIHALNETE
                     IEIIRR"
     gene            888..1547
                     /locus_tag="VGT44_21095"
     CDS             888..1547
                     /locus_tag="VGT44_21095"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV2583369.1"
                     /translation="MHTTIDESQVLSAVYDEVQQRFAAFNDPAHGWEHIERVYSLAIH
                     LARQESADGFIVGMAALLHDLGRAAHDESRHHADLSVEQASDLMARYQLPASAQEAIV
                     HAIAAHSFSRGIQPRTPEARVVRDADRLDSLGAIGIARWAITGTMRRSLLTRTYNPLD
                     PFGEHHMLDDERYMLDHFYSKLLKLVEGMSTETGRRLAEERTLFMRLYLDEFRRELAI
                     L"
     gene            complement(1548..>1991)
                     /locus_tag="VGT44_21100"
     CDS             complement(1548..>1991)
                     /locus_tag="VGT44_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583370.1"
                     /translation="EKMCLRFIREGALPMLPTMQNESLQSFAEKITQTRMDLRLAKRA
                     GLRSTAHKPASSLYSVPPTLEPVQEQDRLAEVPLEEREYLMEPLVLKEGAIEPEIPME
                     TIVDDEGDDTWLNDRVPIWREPAPHLPILKEQSIDDQALTCWLSL"
BASE COUNT          427 a          584 c          518 g          462 t
ORIGIN      
        1 gcggcgaatg atttcgattt ctgtctcgtt cagggcatga atggagggtc ggctggtcgc
       61 ggtagcatcg gacaggcgac gatactcgtt gacaagttcg ccggtcagct cggacgaaat
      121 gaacgttccc ccttcatgaa ccttgcgaat cgcctgcgcc acttcctcca gcgttgcgct
      181 tttgagcagg tagcccctgg caccactgcg catggcgcgc aggatatgct gctgctggcg
      241 aaagaccgtc aacataatta ccgccaccga gggcagttct tcgaccatct ggcgcgttac
      301 ggcgatccca tcgacagccg gcatactgat atccattaac accacgtccg ggcgcagact
      361 gtgtgccgct tgcagcgcct cctggccatt cgccgccccc gcgatcacga cgatatcgtt
      421 ctccaactcc agcaggcggc gcagaccttc gcgcaccagg acgtgatcgt ccacgagcaa
      481 aaggcggatg gtcgcaacat ccccgtcgcc agtcgttgtg acctgttcga attcatcaac
      541 acccgactgc ctatcggtga atgcctgatc tatctggccg agtgctacct cggaattggc
      601 tttatcggta gcaacaaccg tttgttgcct ttgttgtatt tgcgacgttc tctctcttcg
      661 attgcgttgc acgaatggct cctcttcact cacgtatacc agcgcatgag tgaatgcgct
      721 cacatgccag cacgcctgtc tggtgacaaa ccagccgcgc atgtggttta atgagacgaa
      781 tctcatatcc agtatgacca accaggcggt gttctgcatc actcacctgg gggatttctt
      841 gcattcgttc gctcctcgtt ttacttgaaa ggagacgatt tccaggtatg cacacaacga
      901 tagacgaatc acaggtctta tcggctgtgt acgacgaagt acagcagcgt tttgctgcgt
      961 tcaatgatcc tgcgcacggg tgggaacata tcgagcgcgt gtattctcta gccatccatc
     1021 tcgcgagaca ggagagcgca gatggcttta ttgtcggcat ggccgccctg ctgcacgacc
     1081 ttggccgtgc ggcacatgac gagtcccgac atcacgcaga cctctcggtt gagcaggcca
     1141 gcgacctcat ggcgcgctac cagcttcccg cgtcggcgca agaagccatc gtccatgcta
     1201 tcgccgcgca cagcttcagc cggggcatcc agccacgcac accggaagcg cgcgtcgtgc
     1261 gcgacgcaga ccgcctcgat agcctgggcg ctattggcat tgcgcgctgg gccatcaccg
     1321 gaacaatgcg acgctcacta ctgacgcgca cctataatcc actcgatccg tttggggaac
     1381 accacatgct cgacgatgag cgctatatgc tcgaccactt ctacagcaaa ttgctcaaac
     1441 tggtcgaagg aatgtctacc gagacaggcc gcaggttggc cgaagagcgc acattattta
     1501 tgagactgta ccttgatgag ttcaggagag aacttgctat cctgtaatca caacgagagc
     1561 cagcacgtca gggcttgatc gtctattgac tgttccttaa ggataggtag atgcggagca
     1621 ggttcgcgcc agatcgggac tctatcgttc agccatgtat catcgccctc atcatcaacg
     1681 atcgtttcca ttggtatctc aggctcgatt gctccctctt ttaacaccag cggctccatc
     1741 aggtactcgc gctcctcaag cggtacttct gccaatctat cctgctcttg aacaggctct
     1801 aacgttggcg gcacagaata gagagacgaa gctggtttat gcgcagtgga gcgcagcccg
     1861 gcgcgctttg ccaggcgtaa atccatgcgt gtctgtgtta ttttttcagc gaaggattgc
     1921 aacgattcat tttgcatagt tggtaacata ggtaaagcgc cttcacgaat aaagcgtaaa
     1981 cacattttct c
//
LOCUS       DASZAK010001196         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_670917, whole genome shotgun sequence.
ACCESSION   DASZAK010001196 DASZAK010000000
VERSION     DASZAK010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670917"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VGT44_21105"
     CDS             <1..286
                     /locus_tag="VGT44_21105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583371.1"
                     /translation="AGLVIPTRGAPPEKSLDVGRCWFRLTPEQVQSFQQRLEDLCMEY
                     GALSQNAPAEGTQFYEVLQAFYPTLEHSPTVGEGQMERGQWPTPPPKTDD"
     gene            302..1588
                     /locus_tag="VGT44_21110"
     CDS             302..1588
                     /locus_tag="VGT44_21110"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583372.1"
                     /translation="MASADTNSLKRTKTIDFWKFWTGETISNLGSSFTLFALPLLVFK
                     LTGSALNLGIATAAAFLPYLLFGLVIGAWVDRLDRKRMMILVDIGQALVIASIPVMFL
                     LGALSVWWVYGVAFVSTTLKIFFDAGEFATIPSLVDQDDLVTANGRIQASFFGASILG
                     PLLAGVLLFVMPLPTLMFIDASSFLVSACTLSLIFTSFDVAEKREKKSIRHDVAEGLR
                     YVLHHPVLRNISMMMALVNFVGSTTYAQLVLFSERQLHATDSQYSLMNAAGSAGVVVL
                     SLLAGQFRKRWSFSRVALTALATEGLLTIALAFTPWFWLAVPLWALIQGIGILFNINT
                     GSLRQAIVPNQMLGRVLTIASVLAWSAIPLGTLLGGAIIKATGNVMLVYAGIGVLIFL
                     IPTAFSFTALGHAERYLPKKEEQAEASVPSMAPGSR"
BASE COUNT          294 a          514 c          502 g          365 t
ORIGIN      
        1 cgccgggctg gttattccta ccaggggcgc accgccagag aaatcgctgg atgttgggcg
       61 ctgctggttt cggctaaccc cggagcaggt acagtcgttc cagcagcggc tcgaagacct
      121 ctgcatggaa tatggagcat tgagccagaa cgcgccagcc gaaggcacgc agttctacga
      181 agtcttgcag gcgttttatc caacacttga acattccccc actgtcgggg aagggcaaat
      241 ggagcgtgga caatggccca cgccgccacc gaaaaccgac gactaacaga aggagcatgc
      301 tatggcatcc gccgacacca actccctcaa acgcacgaag accatcgact tctggaagtt
      361 ctggacgggc gagacgatct ccaacctggg cagctcattc accctgttcg ccctgccgct
      421 gctcgtcttc aagctgaccg gctcggcgct gaacctgggc atcgccactg ccgccgcctt
      481 cctgccctat ctgctctttg gcctggtcat cggcgcgtgg gtggaccggc tggaccgcaa
      541 gcgcatgatg attctggtcg atattgggca ggcgctggtc atcgcctcca ttccggtcat
      601 gtttttgctc ggcgcgctca gcgtctggtg ggtctatggt gtggcctttg tcagcaccac
      661 gctcaagatt ttcttcgatg ccggtgagtt cgcgaccatt cccagcctgg tcgatcaaga
      721 cgatctggtg acagccaacg ggcgtatcca ggccagcttc tttggggcca gcatcctggg
      781 gccgctgctg gcgggcgtgc tgctgtttgt catgcccctg cccacgctca tgtttatcga
      841 cgccagctcg tttctcgtct cggcctgcac cttgtcgctg atcttcacca gcttcgacgt
      901 cgccgaaaag cgcgagaaga agagcatccg gcacgatgtt gccgaggggc tgcgctatgt
      961 gctgcatcat cccgtgctgc gcaatatctc gatgatgatg gcactggtca actttgttgg
     1021 cagcaccacc tacgcgcagt tggtgctgtt ttctgagcgg cagcttcacg caaccgacag
     1081 ccagtacagt ctgatgaacg cggcaggcag cgcgggcgtg gtggtgctat cgctgctggc
     1141 ggggcaattc cgcaaacgct ggagcttcag tcgcgtagcg ctcaccgcgc tggcgacgga
     1201 ggggctgtta acgattgcgc tggccttcac gccttggttc tggctggctg tgccgctttg
     1261 ggcgctgatt cagggcatag gcattctctt taacatcaac accggctctt tgcgccaggc
     1321 gattgtgccc aaccagatgc tgggccgcgt tctaacgatt gccagcgtgc tggcctggtc
     1381 ggcgatcccg ctaggcacac tgctgggcgg cgcgatcatc aaagccactg gcaatgtgat
     1441 gctggtctac gccggcatcg gcgtgctgat cttcctgatt cccaccgcgt tttcgttcac
     1501 ggcattgggc cacgcggagc gctatctgcc caagaaggag gagcaggctg aggcgagcgt
     1561 cccgtcaatg gcccccggat ctcggtgagt gcgtaacccc gctcgtggag ggctgcaagc
     1621 aactgttcca catgcttacg gtcacgtgtc tccagcgtga gagtcacttc cacat
//
LOCUS       DASZAK010001197         1633 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_206398, whole genome shotgun sequence.
ACCESSION   DASZAK010001197 DASZAK010000000
VERSION     DASZAK010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206398"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /gene="manB"
                     /locus_tag="VGT44_21115"
     CDS             complement(<1..1217)
                     /gene="manB"
                     /locus_tag="VGT44_21115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909030.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEV2583373.1"
                     /translation="MNTAPVPVDPTLFKAYDVRGIAGENLTEEVAYRIGRAAAHYLNV
                     PEIAVGRDMRLSSPQLAAALLRGIIDQGTNAIDLGMVTTDGLYFAVGAFDYPAGVMIT
                     ASHNPGQYNGMKFCRAKAFPISLDSGLAAIRDLAVRGDFPTPVHTGQVTKHEIIDAYV
                     KHVLSFIDVNKVKPLKVVIDAGNGMAGLVLPRVFRHLPCELVPLYFELDGSFPHHPAS
                     PIEPENMIDVQQRVREVGADLGAAFDGDADRMFPVDEQGTIVDGSMVTALVSENLLHK
                     HPGSTILYNLIVSKSVPELITKAGGKAIRTRVGHSYIKEEMRKRYAIFGGEHSGHFYF
                     RDNWYADSGLIALLLMLELVSAESKPLSYLLKPFDQWVRSGEINSTVSDVQGKLHALE
                     ERYSKEAESIDHL"
BASE COUNT          365 a          474 c          443 g          351 t
ORIGIN      
        1 tccaggtgat cgatggattc tgcctccttg ctataccgct cctctaacgc atggagcttg
       61 ccctggacat cgctcaccgt gctgtttatt tcgccactac gcacccactg atcgaatggc
      121 ttgagcaggt atgagagggg cttgctttcg gctgacacca gttccagcat gagtagcaat
      181 gcaatcagac ccgaatcggc ataccagttg tcacggaagt agaagtggcc agagtgttca
      241 ccgccaaaga tggcatagcg cttacgcatc tcctccttga tgtaggagtg ccccacgcga
      301 gtacggatgg ccttgccccc tgctttggtg atgagttcag gtacgctttt cgacacaatc
      361 aagttgtaga gaatggtcga gccggggtgc ttatgcagca aattctctga gaccagcgcc
      421 gtcaccatcg agccatccac gatggtgccc tgctcgtcca cagggaacat ccgatccgca
      481 tcaccatcga aggcggctcc cagatcagcc ccgacctcgc gtaccctctg ttgcacgtca
      541 atcatgttct caggctcgat gggactggca ggatgatggg ggaagctgcc gtccagttca
      601 aagtagagcg gcacgagctc gcagggcaga tgcctaaaca cacgaggcag caccagcccc
      661 gccatgccat tgcccgcatc gatcaccacc ttaagtggct tcaccttgtt cacgtcaatg
      721 aacgagagga catgcttgac ataggcatca atgatctcgt gtttcgtcac ctgcccggtg
      781 tggacaggtg tgggaaagtc tcctcgaacc gccaggtcgc ggatagcagc caggccggag
      841 tccaggctga tggggaaggc cttggcacgg cagaacttca tgccattata ctgcccggga
      901 ttgtgcgagg cggtaatcat caccccggca ggatagtcga atgcccccac ggcaaagtac
      961 agtccatcgg tcgtgaccat gcccaggtct atcgcgttcg taccctgatc aatgatacca
     1021 cggagcaggg cggcggccaa ttgcggtgat gaaagccgca tatcgcggcc cacggcaatc
     1081 tccggcacgt tcaagtagtg agcggcagca cggccaatac gataggcgac ttcctccgtg
     1141 agattctcgc cggcgatgcc gcgcacatca taggctttaa atagagtcgg atcaacagga
     1201 acaggtgcag tattcatctc caaatccttt gctctagcag gtcccttgcc gcctgtacat
     1261 cggcctcgcc aggtttcaga gagaacgcgc cgctctctcc atcgagtatg cacggcgtgc
     1321 cgttgggaat atcgagcacc acatcggctg ctctggcgga caaccagatc tagcgctcat
     1381 caatcgtgat catcatcgcc tccaaaaggg ttcatgagaa agggcccatt ctatggtggg
     1441 gtcacgagat cctctcgacg tttttgtgcg cttaagaact agcacggctc ggaaagccgc
     1501 ttcaaagaca ttaccccttc ttcccagcgc gctcattgct cgatggttgt cacactggta
     1561 tctctgagga gtgtagctga ccgcaagggg acaagacagt acccaaaagg agtaattcct
     1621 gggtgatttt tgc
//
LOCUS       DASZAK010001198         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_386921, whole genome shotgun sequence.
ACCESSION   DASZAK010001198 DASZAK010000000
VERSION     DASZAK010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386921"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1389
                     /locus_tag="VGT44_21120"
     CDS             <1..1389
                     /locus_tag="VGT44_21120"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEV2583374.1"
                     /translation="YEDGHTLSGQSPTRVAAVLSDLGVDVMGANCSVGPAVTLDALQE
                     MIAAIGEQRGAGSARKQLFSAQPNAGLPSRVGHRFFYVSTPDYFAEYALQFAKAGVRL
                     IGGCCGTTPHHIAAMRKAIDAAYAPAATPLLNGKHRTAPIEISSGASTIARLIEEEVI
                     LPQQGSMTHVQEKLAAGEFVVSVELDPPKGLNPAKILEGAALLQKAGVDCINIADSPM
                     ARVRMSCIALARLIQDHLGIETIIHFTTRDRNLMALQSELLGAHALGIRNILALTGDP
                     LHVGDYPGTTGVWNVDSVGLIQVIRGMNDGHDAAGSSIGARASFHIGAALNLNMTGEQ
                     TEQEIEKYRRKIDAGAHFIMTQPIYEIAPLQRFLDRVGTPPVPIILGCIPLHSSRHAE
                     YLHNEVPGITIPDDVRLRMKQAGDQGHEEGLRQAHEMLSETRHLVQGVYLMPSYGRYD
                     IVGRLASMLHDR"
     gene            1539..2264
                     /gene="infC"
                     /locus_tag="VGT44_21125"
     CDS             1539..2264
                     /gene="infC"
                     /locus_tag="VGT44_21125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV2583375.1"
                     /translation="MRETRVNERIRAHQVRLIDENGENVGVMPPQRALDIARERNLDL
                     VEISPNANPPVCKLMDYGRYKYEQAKKENEARKNQKTITLKEIRLSPRTDDHDIDVKT
                     RKIQEFLADGDRVRVSVKFRGAEMRHPDIGRKLLDGIAEVLKGTAVLERPPIMEGKMM
                     SMIVSRAPGWEPPKKQPAAPAVASKKHQAQPAEEAEPAQEAEQTASPAQTDVQTPTEP
                     IEQELSPALVSERKEAATASQEN"
BASE COUNT          557 a          688 c          687 g          435 t
ORIGIN      
        1 tatgaggatg gccacacgct ctccggccag tcgcccacgc gcgtcgccgc cgtgttgagc
       61 gacctgggcg tagatgtgat gggcgcgaat tgcagtgtgg ggccagccgt gacgctggat
      121 gccttgcagg agatgatcgc cgcgattggc gaacagcgcg gagcaggttc agcgcgcaag
      181 cagttgtttt cggcgcagcc caacgcgggt ctgccctcgc gcgtcggcca ccgtttcttc
      241 tatgtctcga cgcccgatta ctttgccgaa tacgcgctcc aattcgcaaa ggccggcgtc
      301 aggctgattg gaggctgctg cggcaccacc cctcaccata tcgccgccat gcgcaaagcc
      361 atcgacgctg catatgcgcc agcagccaca cctctgctga acggtaagca tcgcacggct
      421 cctatcgaaa ttagctccgg cgcgtctacg attgccaggc taatcgagga agaagtcatc
      481 ctgccgcaac agggatcgat gacgcatgta caagaaaagc tggcagccgg tgaatttgtg
      541 gtgagcgttg agctggaccc acccaaggga ttgaaccccg ccaaaatcct tgagggcgcg
      601 gcgctattac agaaagcggg ggttgattgc atcaacatcg ccgacagccc catggcgcgc
      661 gtgcgcatga gctgtatcgc cctggcgcgc ctgatccagg atcacctggg catcgaaacc
      721 atcatccact tcaccacgcg cgaccgcaac ctgatggcgc tccaatcgga gctgctgggc
      781 gcgcacgcgc tcggcatccg caatatcctg gcgctgacgg gcgatccgct gcacgtgggc
      841 gattatcctg gcacgacggg ggtctggaac gtcgattcgg tgggcctgat ccaggtcata
      901 cgcggcatga atgatggaca tgacgcggcg ggatcatcga tcggggcacg cgcttcgttc
      961 cacatcggcg cagccctgaa cctgaacatg accggggaac aaaccgaaca ggagatcgag
     1021 aaataccggc gcaagatcga cgcaggcgcg cattttatca tgacccagcc gatctacgag
     1081 atcgcgccgc tgcagcgttt cctggacagg gtcggcacac cccccgttcc aatcatcctg
     1141 ggatgcatcc ctctgcacag ctcgcgccat gccgaatatc tgcataatga agtgccgggc
     1201 atcaccatcc ctgacgatgt gcgcctgcgc atgaagcagg cgggcgacca ggggcatgaa
     1261 gaaggtctgc gccaggcaca tgagatgttg agcgagacgc ggcatctcgt gcagggtgtg
     1321 tacctcatgc cctcctacgg acgctacgac attgtgggca ggctggcaag catgttgcac
     1381 gaccggtaaa atttgactag tcccatgtag tatgctacac tgtatatggt atcttgggct
     1441 tagtctgtaa ttcatcaaag tagtgttttt acaccatgta ccatcaccat aaggaggaat
     1501 agcctattaa cagcagggat cttcggggga atgaccgggt gcgtgagacg cgagtcaatg
     1561 agcgtatccg ggcgcatcaa gtacgcttga tcgatgaaaa tggcgaaaat gttggagtga
     1621 tgcccccaca acgtgcgctc gatatcgccc gcgaacgcaa ccttgacctg gttgagatct
     1681 cacccaatgc gaacccgccg gtctgcaaat tgatggacta tggccgctac aaatatgagc
     1741 aggccaaaaa agagaacgag gctcgcaaga atcaaaagac catcacgctc aaagaaattc
     1801 gcctcagccc gcgcaccgat gaccatgata tcgatgtaaa gacgcgcaaa atccaagaat
     1861 tcctggccga tggcgatcgt gtgcgtgtca gcgtcaaatt caggggcgcg gaaatgcgtc
     1921 acccggatat cgggcgaaag ctgctggatg gcatcgcgga agtcctgaag ggaacagccg
     1981 tccttgagcg cccgccaatc atggagggca agatgatgtc gatgattgtc tcgcgcgctc
     2041 ctggctggga accaccaaag aaacaacctg ccgcccccgc tgttgccagc aagaagcacc
     2101 aggctcaacc agcggaggaa gcagaacctg ctcaggaggc agagcaaaca gcttctcccg
     2161 cccagaccga tgtgcaaacc cctaccgagc cgatcgagca ggagctatcg ccagcccttg
     2221 tttcggagag gaaagaagcg gcaacggctt cacaggagaa ttaacctggc gtcgagcgcg
     2281 tacaaaaaag ccagggcgac atacgcggta tgtcgccctg gcttttttcg tcggatttca
     2341 tgaaattcaa taaccaacat cgatgct
//
LOCUS       DASZAK010001199         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_386931, whole genome shotgun sequence.
ACCESSION   DASZAK010001199 DASZAK010000000
VERSION     DASZAK010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386931"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1211)
                     /gene="mnmE"
                     /locus_tag="VGT44_21130"
     CDS             complement(<1..1211)
                     /gene="mnmE"
                     /locus_tag="VGT44_21130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV2583376.1"
                     /translation="MQNDTIAAIATPQGYGGIGIVRVSGSEAFAIVLPLLRRHGKGDH
                     SELPLPPSHQLTYGHIVVPTTQEILDETLVTFMRAPHTYTREDVVEIQGHGGPLVLQR
                     ILLLVLAQGARMANAGEFTLRAFLNGRLDLAQAEAVMDLISSQTEMGHRLAIQQLQGR
                     ISEQVQDARHDLLGVIARIEASIDFPEEEVPQPQPEELAPLILAAQSKVQRLLAGFEQ
                     GRLYKQGLRTAIIGRPNVGKSSLLNALLRSERAIVTPFAGTTRDTVEEVANVRGLPLH
                     LIDTAGITAPSASSDPVEQIGVERSRAAAESADLVLLVFDGSEPLNEQDRRVSDEVWS
                     MGFGGRNNEIDQDFARRRPAIIVINKTDSRQQVEIEQLKQMWRDAPFAHTSALTGAGL
                     PELEEAIVNLAL"
     gene            complement(1219..>2070)
                     /gene="fdhD"
                     /locus_tag="VGT44_21135"
     CDS             complement(1219..>2070)
                     /gene="fdhD"
                     /locus_tag="VGT44_21135"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEV2583377.1"
                     /translation="VTSMQVTHWQEQKQQPREEMLTVEEPLEIRLNQHSLAVIMRTPG
                     HDRELAAGFLFTEGIISSSDDMLSLEEAVDADGLPLANVIDATLRPAEERPNDGQEQR
                     ITAAFERHFAVSASCGLCGKNSIADLMLNVAPLASDDLYIAASTLYKLSGQLREQQAV
                     FTHTGGLHAAGLFTTTGELLLLREDVGRHNAVDKLIGHGLLHGALPYSYHILLVSGRT
                     SFEIIQKALLARIPCVAAISAPSSLAVDLASEGGMTLIGFLRDHSMNVYTHPERIDAC
                     PPEAHRV"
BASE COUNT          391 a          589 c          648 g          442 t
ORIGIN      
        1 agcgccaggt tcacgatagc ctcttccagt tctggcagtc cagctccggt gagagccgag
       61 gtatgcgcaa aaggcgcatc gcgccacatc tgtttgagct gctcgatttc cacctgctga
      121 cggctatctg ttttattaat aactatgata gcgggtcttc ttcgcgcaaa atcttgatca
      181 atttcgttgt tcctgccgcc aaaacccatc gaccagactt catccgagac gcgcctgtcc
      241 tgttcgttga ggggttccga gccatcgaag accagcagca ccaggtcggc gctttcggcg
      301 gcggcgcggc tgcgttccac gccgatctgc tcaacagggt cgctgctcgc tgagggggca
      361 gtaatgcccg ccgtatcgat caggtgcagc ggcaagccgc gcacgttggc aacctcttca
      421 actgtatcac gtgttgtccc ggcgaagggt gtcacgatgg cgcgctctga gcgcagcaga
      481 gcgttcagca ggctggattt gcccacgttg ggtcgtccaa taatagcggt gcgcaatccc
      541 tgtttgtaaa gtcgcccctg ctcgaagcct gccagcaagc gctggacctt gctctgcgcg
      601 gcgaggatca gcggcgcgag ttcttccggc tgcggttgtg gcacttcctc ttcagggaag
      661 tcgatgctgg cctcgatgcg cgcgataacg cccagcaggt cgtggcgggc gtcctgtacc
      721 tgctcggaga tgcgcccctg gagttgttgt atagccaggc gatgccccat ttcggtctgc
      781 gagctaatca agtccatgac cgcctcggcc tgcgccaggt caagtcggcc attcaggaaa
      841 gcgcgcaagg tgaactcacc cgcgttggcc atgcgcgcgc cctgggccag cacgagaagc
      901 aggatgcgtt gtaggaccag cggcccgccg tgaccctgaa tctcgacgac atcttcgcgt
      961 gtgtaggtgt gcggcgcgcg cataaaggta acgagcgtct catccaggat ctcttgagtg
     1021 gttggcacga caatatgccc gtaagtcagc tggtgcgatg gcggcagcgg aagttccgaa
     1081 tggtcgccct tgccatgacg ccgcaggagc ggtaagacga tggcaaatgc ttcggagccg
     1141 ctcacgcgga cgatgccaat cccaccatac ccctgtggtg tggcaattgc tgcaatcgta
     1201 tcattctgca tataacaatt atactcggtg tgcctctggt gggcaagcat ctatgcgttc
     1261 tggatgggta tacacgttca ttgagtggtc gcgcaaaaag ccgatcagcg tcatgccgcc
     1321 ctcgctggcc agatcaacgg cgagactgga tggagcggag atggcggcta cgcaggggat
     1381 gcgagccagc agggctttct ggatgatctc gaacgaggta cgtccactga ccagcaggat
     1441 gtgataggaa tacggcagcg cgccgtgtag aagtccgtgg ccgatcagct tatcgacggc
     1501 gttgtggcgg cctacgtcct cgcgcagcag aagtagctct ccggtggtgg tgaatagtcc
     1561 ggcggcgtgc aggccgccgg tatgtgtaaa gacagcctgc tgctcgcgca attggccgga
     1621 aagcttgtag agggtagatg cggcgatgta gagatcatcg cttgccaggg gagcaacgtt
     1681 gagcatcagg tcggcgatgc tgttctttcc gcacaggccg cagctggctg agaccgcgaa
     1741 atgtcgctcg aacgcggcgg taatacgctg ctcctgccca tcattgggac gttcttcagc
     1801 ggggcgcagt gtcgcgtcga tgacgttggc gagcggcagg ccatcagcat cgacggcttc
     1861 ttcgagagaa agcatatcgt cgctactgga aatgatgcct tccgtgaaga ggaagcccgc
     1921 cgccagctcg cgatcgtggc cgggtgtgcg cataatcacc gccaggctgt gctggttcag
     1981 gcgaatctcc aatggctctt cgacagtcag catttcctcg cgtggctgtt gcttttgctc
     2041 ctgccagtgt gtcacctgca ttgatgtgac
//
LOCUS       DASZAK010001200         2971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_458551, whole genome shotgun sequence.
ACCESSION   DASZAK010001200 DASZAK010000000
VERSION     DASZAK010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458551"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VGT44_21140"
     CDS             complement(<1..497)
                     /locus_tag="VGT44_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2583378.1"
                     /translation="MEVTWSLGETTVYGTLVRPGGTGPFPAVVMVAGSGPTDRDWNSP
                     LLPGTNGSARLLAEALARAGIASLRYDKRASGPHARENMQSLIGKLSMQSHVDELAGA
                     VRTLSSQAYVRDGRIFALANSEGTLHALNYQLHNPAIPFAGLVLTGPPGRAVGTVARS
                     QLAAQA"
     gene            complement(691..>2971)
                     /locus_tag="VGT44_21145"
     CDS             complement(691..>2971)
                     /locus_tag="VGT44_21145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEV2583379.1"
                     /translation="SIGLGHIATQLREARERIVQSEASLASSQKESAVARPERPGAVE
                     RRRKLIDIGQYRFRRDGEYHAYNPLIIRALQKAAQSGEVEDYRRFTELVYQRPPTVPR
                     DQFAFAPTTPIPLEQVEPLESIRVRFVISAMSLGALSPEAHRAIAAAMNSIGGRNNTG
                     EGGEDPNWYHETLNGAPVSSKIKQVASARFGVTPEYLVRAEELEIKMAQGSKPGEGGQ
                     LPPEKVTPFIARLRHTAPQVALISPPPHHDIYSIEDLAQLIYDLKQVNPFARVGVKLV
                     SSLGVGTIAAGVAKAHADYVQISGYDGGTGASPIQSIKHAGIPWERGLAETQQVLARN
                     GLRGRVKVRVDGGLKTGRDVVIAAMLGAEEFGFGTGALVSLGCDMARQCHLNTCPTGI
                     ATQREDLRAKFTGTPQMLINYLTLVAQEVRELMAMLGVSRLDDLVGRADLLQATGDTG
                     IDLAQLLVPVPESSSPSTKAGIPNKLPQSKLAERLLEDAQQAINGGNSVLIQQVIHND
                     DRTIGAGLAGEIARRYGNTGMPSATITCFLQGSAGQSFGAFSVPGMRLILHGEANDYV
                     GKGMSGGEIVLAPPADARFAAHENVILGNTVLYGATGGQLFAAGRAGERFAVRNSGAL
                     AVVEGVGAHGCEYMTGGVVVILGDTGRNFAAGMSAGAAYVLDMNGTFPARCNTELVEF
                     QRIDDPDESEAVRTIIQWHSKKTRSWRAAQILSEWSRIQRCFWRVLPRGSAFSALDYV
                     NAQEGCHTGQFVEAFQYAR"
BASE COUNT          537 a          954 c          899 g          581 t
ORIGIN      
        1 gcctgcgcag ccaattggga tcgcgccacc gtgcctactg cccgtccagg agggccggta
       61 agcacgagtc ccgcaaacgg aatcgcgggg ttgtgcagtt ggtagttcag ggcgtgcagg
      121 gtcccctcgc tattggcaag cgcgaagatc cggccatcgc ggacatacgc ctggcttgac
      181 agggtacgga cggctccggc gagttcgtcg acatgacttt gcatgctgag cttcccaatc
      241 agggattgca tgttctctcg agcatgaggg ccggaagcac gcttgtcgta gcgtaaggaa
      301 gcgatccctg ccctcgcgag cgcctccgca agcagacgcg cgctgccatt ggttcccggc
      361 agcagtggcg aattccaatc gcggtcagtg gggccactgc ctgccaccat caccaccgca
      421 ggaaacggtc cggtgccacc tggcctgact aacgtgccat acacggtcgt ctcgcctagc
      481 gaccaggtaa cttccaagct ctctccatat atatcctgat cagtcattct tcacatcctt
      541 tcacggttct tatgcgtcct caatttcaat acttcaacac gtattgaaat agtaacgcaa
      601 aaaagtccca tagtcaagga gtagtaccga gacgaagcgt gtcaactggc ataatgaagc
      661 ggtgagagac tgtcttattt cccacctgca tcagcgggcg tactggaacg cctccacaaa
      721 ttgcccggta tggcatcctt cctgcgcatt gacgtagtcc agggcggaaa atgcactgcc
      781 gcgtgggaga acgcgccaga agcagcgttg gatgcggctc cattcggaca ggatttgcgc
      841 tgcgcgccag ctgcgcgtct tcttgctgtg ccactggata atggttcgca cagcctcgct
      901 ttcgtcggga tcgtcgatgc gctggaattc caccagttcg gtattgcagc gcgccgggaa
      961 ggtgccgttc atgtccagga cgtaggccgc gcccgccgac atgcctgccg cgaagttgcg
     1021 cccggtatcg cccaggatga cgaccacgcc gcccgtcata tactcgcagc catgcgcgcc
     1081 gactccctca acgaccgcca gcgcgccgct attgcgcacg gcgaagcgct caccggcgcg
     1141 acccgccgcg aagagctgcc cgcccgtcgc gccgtagagg acggtattgc cgaggatcac
     1201 gttttcgtgc gctgcgaaac gcgcatcggc gggcggcgca agcacaattt caccgccgct
     1261 catacctttg ccgacgtagt cgttggcctc gccatgcagg atcaggcgca tcccaggcac
     1321 cgaaaacgcg ccgaagctct ggccggctga cccctgcaag aaacaggtga tggtcgcgct
     1381 cggcataccg gtgtttccat agcggcgcgc aatctcaccc gcgagtccgg cgccgatggt
     1441 gcgatcatcg ttgtgaatca cttgctggat caagacactg ttgcctccgt tgatggcttg
     1501 ctgcgcatct tccagtaagc gctcggccaa ctttgactgc ggcagtttgt tggggatacc
     1561 ggcttttgtc gagggcgacg aggattcagg caccggaacg agcagttgcg cgaggtcgat
     1621 accagtatct cctgtggcct gcagcaggtc agcgcggcct accaggtcat ccaggcgcga
     1681 gacgcccagc atcgccataa gttcgcgtac ctcttgcgcc accagggtca ggtagttgat
     1741 gagcatttgc ggcgtgccgg tgaacttggc gcgcaggtct tcgcgctgtg tcgcaatgcc
     1801 cgtcggacag gtgttgaggt gacactgacg cgccatgtcg caccccagcg acaccagcgc
     1861 gcccgtgcca aagccgaact cttccgcgcc tagcatcgcc gcgattacga catcgcgccc
     1921 ggtcttcagg ccgccatcga cacgaacttt gacgcgcccg cgcaggccgt tgcgagccag
     1981 cacctgctgc gtttccgcca ggccgcgctc ccaggggata cccgcgtgtt tgattgactg
     2041 gatgggcgaa gcgcctgtcc cgccatcgta gccgctgatc tgcacgtaat cggcatgtgc
     2101 cttcgccacg cctgccgcaa tggtccccac acccaggctg gataccagct tgacgccaac
     2161 gcgcgcgaat ggattgacct gtttcaagtc atagattaac tgcgccaggt cttcgatgct
     2221 atagatgtcg tgatgcggcg ggggcgagat caatgcgacc tgcggcgcgg tatgacgcag
     2281 gcgcgcgatg aagggcgtca ccttctccgg tgggagttgg ccgccctcgc ccggcttcga
     2341 tccctgcgcc atcttgatct ccagctcttc agcgcgcacc aggtattcgg gcgtcacgcc
     2401 gaaacgcgcc gaggccacct gcttgatctt gctgctgacg ggcgcgccat tgagcgtttc
     2461 gtgataccag ttggggtctt cgccgccttc accggtgttg ttgcgcccgc cgatactgtt
     2521 catcgcggcg gcgatggcgc gatgcgcctc cgggctgagc gcgccgagtg acatggccga
     2581 tatgacgaaa cgcacgcgaa tcgactccaa cggctcaacc tgctcgagtg gaatgggcgt
     2641 ggtcggcgcg aaggcgaact ggtcgcgcgg cacggtcggc gggcgctggt agaccagctc
     2701 ggtgaaacga cgatagtcct ccacctcgcc gctctgcgcc gccttttgca gggcgcggat
     2761 aatcaagggg ttataggcgt gatattcgcc gtcgcgccgg aaacggtact ggccgatgtc
     2821 gatcaacttg cgccgccgct caaccgcgcc gggtcgttca ggcctggcga cagccgactc
     2881 cttttgcgag cttgccaggc tcgcctcgct ctgcacgatg cgctccctgg cctcacgcaa
     2941 ctgcgtggca atatgtccca ggccgatcga g
//
LOCUS       DASZAK010001201         3194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_636314, whole genome shotgun sequence.
ACCESSION   DASZAK010001201 DASZAK010000000
VERSION     DASZAK010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3194
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636314"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2156)
                     /locus_tag="VGT44_21150"
     CDS             complement(<1..2156)
                     /locus_tag="VGT44_21150"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEV2583380.1"
                     /translation="MSTQERPAQVGVSLPRQDGPDKVTGRARYAGDQTAQGLLYARLV
                     TSPYAHARILRIDTTEALALPGVVAVFTADTLGMAQADALSRAQSPLAQQETLWCGHP
                     VAVVVGETEAVAEDGASAVEVDYEPLPAAIDPEAAILPGAPPARLKAESTAATGGGAP
                     TGIPHATPVEEEGYSPNVSRTPPMRMGDIEAGLREADVVVECYYRTHPVHQSYMETHS
                     VTVAPSPSGHHLVIWPSTQGLFNVRSSVASALNMPERQIRVEPVTIGGGFGGKETLLE
                     PLTAAVAQRLRKPVRLVFTRQEDLLAGNPAPQMVMTIKLGAKRDGTLTAIQARIILDA
                     GAYPNMLAGLSGFHFAAIYRCPNLDIRCDVTQTNKPGTGAYRAPAGPQAYFALESTVQ
                     ELCQKLGMDPLRFRQINALKVGDPNIMRSQWPRIGLLECLEQIEHHPLWAQREQARQQ
                     APQELSGWKIGIGLAAGGWPGGTEPAAALCRLEQDGSFTIVVGSVDISGSDASLALIA
                     ADTLDMPASAVNVTHDSTDSMPYSGLSAGSKTTYTVGSAVLAAAREARAQIIAIAAEM
                     LEAAVEDLELRNGMVQVRGVPDKHITLQQIAGNTMRFGAPYEPILGRGRSANRVSSPM
                     FAVHVAKVAVDPQTGEVRVLDYVAAQDVGRAINPAEVEGQIHGGVVQGIGWALFEGML
                     YDDDGQLLTSTLMDYALPHSADVPTITPLLVEVPSEL"
     gene            2351..>3194
                     /locus_tag="VGT44_21155"
     CDS             2351..>3194
                     /locus_tag="VGT44_21155"
                     /inference="COORDINATES: protein motif:HMM:NF022834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HEV2583381.1"
                     /translation="MSISLFALDYSRPMRALFEQAQRAGTIHSVFRRATNIALDETLV
                     TILSSELPRMPNGLRLPSLTANELLSSLQPGMRVWIGDGRLTIPSQNCTLWLPEDAAW
                     EPVPDVSSGQWHCETVTRHATMLAHYLSRVATDEGLASLVRPLLLGQSAEETPLARIA
                     LPALRLLECASREQDIAHVEEAASRLAGLGPGLTPSGDDALGGFIAILALLSQYMSDA
                     AAPCDAIVAAIVRAARPGTGTISAALLAYAARGEVAEPVGALLLALASESPDPVMLAA
                     RHVLA"
BASE COUNT          573 a          987 c          968 g          666 t
ORIGIN      
        1 agttccgacg gcacttcgac cagcaggggt gtaatcgtgg gaacatccgc gctgtgtggc
       61 agcgcatagt ccatcagcgt cgaggtgagc aactgtccgt cgtcgtcata gagcattcct
      121 tcgaacagtg cccagccgat gccctggacg accccgccgt gaatctgacc ctctacctct
      181 gccgggttga tggcgcggcc tacatcctgc gccgcgacat agtcgagcac acgcacctcg
      241 ccggtttgcg ggtcaacggc caccttcgcc acgtgtactg cgaacattgg cgacgagacg
      301 cgatttgccg agcgcccgcg accgagaatc ggctcgtaag gcgcgccaaa gcgcatggtg
      361 ttcccggcga tctgctgtaa ggtgatatgc ttgtctggta ctcccctgac ctgcaccata
      421 ccatttcgca attccaggtc ttcgactgcc gcttcgagca tctctgccgc gattgcaatg
      481 atttgcgcgc gcgcttcgcg agccgccgcc agtaccgcag atccgacggt gtaggtggtc
      541 ttgctcccgg cggacaggcc gctatagggc atcgagtcgg tgctatcatg cgtcacgttc
      601 accgcacttg ctggcatgtc cagcgtgtcc gcagcgataa gggccagcga ggcgtccgaa
      661 ccggagatat cgacactgcc gacgacgatg gtaaagctgc cgtcctgctc caatcggcaa
      721 agggcagcgg ctggctccgt tccgcctggc cagcctccgg cggcgaggcc gataccgatc
      781 ttccacccgc tcagttcttg cggcgcttgc tgccgggcct gctcgcgctg cgcccacaac
      841 ggatgatgtt cgatctgttc aaggcactcc agcaggccaa tgcgcggcca ctggctgcgc
      901 atgatattag ggtcgcccac tttgagggca ttgatctggc ggaagcgcag cgggtccata
      961 ccgagcttct ggcagagttc ctgcaccgtc gattccaggg cgaagtatgc ctgcggacct
     1021 gccggggcgc gataagcgcc cgtgccgggc ttgttggtct gcgtcacgtc gcagcgaata
     1081 tcaaggttgg ggcagcgata gatcgccgcg aaatggaagc cgctcagacc ggcaagcatg
     1141 ttagggtatg cgcctgcatc caggatgatc ctggcctgga tcgcggtaag cgtaccgtcg
     1201 cgcttcgcgc cgagtttgat ggtcatgacc atttgcggcg cggggttgcc ggccagcagg
     1261 tcttcctggc gtgtaaagac caggcgcacc ggcttgcgca agcgctgcgc taccgccgcg
     1321 gtcagtggtt caagcagcgt ctccttgccg ccaaatcccc caccgatagt caccggctcg
     1381 acccgtatct gtcgctcagg catgttgagc gcactggcaa cggaggaacg cacgttgaac
     1441 aatccctgcg tgctgggcca gatcaccagg tgatggccgg acgggctggg ggcgaccgtg
     1501 accgagtgtg tctccatata ggactggtga accgggtggg tgcgatagta gcactcgacc
     1561 accacatcgg cttcgcgcag accggcttcg atatcaccca tcctcattgg cggcgtccgg
     1621 ctcacattcg gggagtatcc ctcttcctca accggcgttg catgagggat gcccgtcggc
     1681 gcgcctcctc cggttgcggc ggtagattcc gctttcaagc gcgcaggtgg cgcgcctggc
     1741 aggatggcgg cttcgggatc gatcgcggcg ggcagtggct cgtagtcaac ctccactgct
     1801 gaggcgccat cctcggcaac ggcctccgtt tcgcccacga caacagcgac cgggtggccg
     1861 caccataacg tttcttgttg tgccaatggg ctttgcgcgc gtgaaagcgc atcagcctgc
     1921 gccattccca gcgtatcagc ggtaaagacg gctacgacac ccggtagggc taacgcttcg
     1981 gttgtgtcga tgcgcaagat gcgcgcgtga gcataggggc tggtgaccag gcgcgcgtac
     2041 aacaatccct gcgccgtctg atctcccgcg taacgcgccc gaccggttac tttatccggc
     2101 ccatcctgtc tggggagcga tacgccaacc tgcgcaggtc gctcctgcgt tgacattcct
     2161 tgcatcattg aggcaagctc ttctttctat tgcaattgac tattgtgact aatgtcttca
     2221 ttggcatttg tcctcatcat atcgtaattt acatcgcttt gtacaggtct ggatgcaaag
     2281 tccactgcct cttctgatat ggtaatatgc aggtagattt catcaacaga cgccttgcga
     2341 aaggtaaagc atgtctattt ccctgtttgc tctcgattac agccgaccaa tgcgcgcgct
     2401 cttcgagcaa gctcaacgcg caggcacaat ccatagtgtc ttccggcggg caactaatat
     2461 tgcccttgat gaaacgcttg tgaccatctt gagcagtgag ttaccacgta tgccgaatgg
     2521 cctgcgactg ccatcgctga cggcgaacga gctgctgagc agcctgcaac cgggtatgcg
     2581 agtctggata ggggatggaa ggctgactat tccttcacag aattgtacgc tctggcttcc
     2641 tgaagatgca gcctgggagc cagtgccaga tgtatcgtcg gggcaatggc attgcgagac
     2701 ggtgacgcgc catgccacaa tgcttgctca ctatctctcg cgtgtcgcga ctgatgaagg
     2761 gctggcatcg ctggttcgac cgctactgct ggggcaatcg gcagaagaga cgccgctcgc
     2821 acgcatagct ttaccggcgc tgcggctgct ggaatgcgcg agccgggagc aagatattgc
     2881 acatgttgag gaagcagcat cccggctggc tggcctcggt cccggcctaa cgccctccgg
     2941 cgacgacgcg ctgggcggtt ttatcgcaat cctggcgctg ctcagccagt acatgagtga
     3001 cgccgccgct ccttgcgatg ctatcgtggc ggccattgtc agggccgcgc gcccaggcac
     3061 cggcactatc agcgccgctt tgctggccta tgctgctcgc ggcgaagtgg ccgagccggt
     3121 cggagcgctt ttactggccc tggcaagcga atcgcccgat ccggtcatgc tcgccgcgcg
     3181 tcacgtcctg gcaa
//
LOCUS       DASZAK010001202         6320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_458582, whole genome shotgun sequence.
ACCESSION   DASZAK010001202 DASZAK010000000
VERSION     DASZAK010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458582"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1690)
                     /locus_tag="VGT44_21160"
     CDS             complement(<1..1690)
                     /locus_tag="VGT44_21160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEV2583382.1"
                     /translation="MKYSRWLVPVIILVMLLSVSVSLFSLYQTRASMAALESVPAVSP
                     LVARSAPIGDAVPSMRLSLSINLSLRNTADLARYVQAEYTPGSYLFHRYLSAAEFAAL
                     YGPTAQDVQQVAGFLRAQGFSVTRAVAGQQVIDFSGTVAQAEQSFGVQIHTYRAGNGR
                     VFYANSAAPRVPLALRPLIANINGLDNATARAHPPLRSPRSISPARSPRSIVCPGPGS
                     DALEYLLPSQFSSAYNFTGAYNAGLRGEGQGIALFELDGYSGSDIAAFQSCYDKNSPT
                     RINNVLIDGGVATPGSGAIEVELDMEVVLGTLNGLTNLFVYQAPNTATGYNDEWNQIV
                     QDDIPVVSTSWGLCEPQMSASDVQAENGFFVQADAQGQSILTAAGDNGAFDCGDGTLA
                     VDDPASNPNITGVGGTHLIVNSSSSYSSESVWSGTPNSFNGGGGGISQLWTMPSYQSG
                     PGVINGSSSGSPCSAPSGQYCREVPDVSLNADPTVGYVVYCTVATAQCDPSAPFIPVG
                     GTSAAAPMWAGVVALTDQYELEHGGTNLGFLNPLLYTLFSSSTLYSRVFHDVTSG"
     gene            1932..2573
                     /locus_tag="VGT44_21165"
     CDS             1932..2573
                     /locus_tag="VGT44_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEV2583383.1"
                     /translation="MSIVKNLRPRAATQVPEPPLARFLFADTRMAWFWLIVRVYVGWQ
                     WLVAGIHKLTGYSLDFSTFGQHTGNSWIFTDHLGAPLSGFLNGVITPANIANPATGWY
                     VWFVQHVAIPGAGVFTYMVTFGEILVGLGLIFGVLTGIAAFFGLFMNLNFLLAGAISI
                     NPIIGGLALFVMLAWRVAGYWGIDRYLLPLLGTPWTGALTRKAQNIPPTPAPA"
     gene            complement(2685..3392)
                     /locus_tag="VGT44_21170"
     CDS             complement(2685..3392)
                     /locus_tag="VGT44_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583384.1"
                     /translation="MSIKKTTILTADDDPQLLRLVTRNLQLEGYDVLPVSDGQQALEQ
                     IEQKSPDLVLLDVMMPRMDGFSVCYKVREFSTVPIILVTARGQDQDKVRGLDLGADDY
                     LTKPFSVDELLARVRAVLRRSEFSAKENTRGLLATASTGDLVIDYSQHLVTLNGKEVP
                     MTPTEYRILAYLAQNAGHIVTQDLLLEHVWGLEYMGESHMLQVNINRVRRKIESDPTH
                     PRYLLTKVGVGYYLASL"
     gene            3555..6227
                     /locus_tag="VGT44_21175"
     CDS             3555..6227
                     /locus_tag="VGT44_21175"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583385.1"
                     /translation="MAILHPRNKLTRRAETALKSQRFARMRTLFHTWRELPYSGVVTL
                     LLSTLLATLLVMLFDRSVVTLVNPGLIYLPIIAMIAYHWKWYLAVIGALLQLFCVYFF
                     FLPPFNSFSVSSEQAIAQLIVLAAAMGFVLAIVQLARYGRSNAERAAARFEALNRIGA
                     ALTSELDESRLLHTIAETARSLTNAGFAAFTLRPINELGEPLVPSEGHLFHLAAVVGV
                     TKEQEQLLRHMPLGGEGLLAPIFHHGMAVRVPDALVYLQQSGGAHGGKVRMTSEDRLL
                     ARQAAFDYAHGNLDRDGLRSMGVPRGHPIVRSFLGVPLLDRAGNVRGGLLLGHSDPDC
                     FTLADEQLLVGLAAQAAIALENARLYSAAQTQARELDAIFESIADGIMLVDEQGEVLR
                     ENRTARHLREQVASMQADQSSPTQQPRVEDLLREAATRAQVDATEPGGIPATIIDNAG
                     DAREFVINASPLRPPSLPLLSEQDNLATHNELKAMNGSVVVWHDVTEAHRLLIEQQAH
                     AETEARRALLQTVIDELPGGVYLVRGHDARLVLANRAIADVWGATWQYDQPMIEFLEQ
                     NDIRLFHFDGRSLTSGELATLRAVRRGESIYHQQEIIRHKDGTALPVLVNAVALDPLV
                     LGWNGESVPAESDPEPAAIVVHQDVTALREAERLKDEFIGIAAHELRTPLAVIKGFAQ
                     MLISQTARGKGPALADWQSEAIQDIDQATIRLVELTEDLLDVTRLQGGRLELHPEPTD
                     LVALAQRIAARVRVTTSKHSVTIHTGTEHLVATIDPRRIEQVLSNLLNNAIKYSPDGG
                     EITITLREDRPTCSAILTVQDNGIGIPAGQQGRIFGRFARADNARDSNISGTGLGLYL
                     CRELVERHNGRIWFESVEGHGSTFYVALPLEKSA"
BASE COUNT         1307 a         1962 c         1772 g         1279 t
ORIGIN      
        1 tcccgctggt cacatcgtgg aagacgcggc tgtacagcgt cgatgaactg aacagcgtat
       61 agagcaatgg attgaggaaa ccaagattgg tgcctccatg ctccagttcg tactggtccg
      121 tcagagccac aacgccagcc cacatgggcg cagccgccga agtgccgcct accgggatga
      181 aaggagcgct ggggtcgcat tgcgccgtgg cgaccgtaca atacaccaca tacccaaccg
      241 tcgggtcggc gttcaacgaa acatcgggaa cctcgcggca gtattgccct gacggtgccg
      301 agcagggaga gccgctggat gagccgttga tgaccccagg gccgctctga taggaaggca
      361 tagtccagag ctggctgatg ccgccgccgc cgccattgaa ggaattcggc gttccagacc
      421 agacgctttc gctgctgtag ctactgctgc tgttgacgat aaggtgcgtg ccgcccacac
      481 cggtgatgtt ggggttgctg gccggatcgt ccaccgccag cgtgccatcg ccacagtcga
      541 acgcgccatt gtcccccgcc gccgtcagga tgctctgccc ctgtgcgtcg gcctgtacga
      601 agaagccatt ctccgcctga acgtcggacg cgctcatctg cggttcgcac aggccccaac
      661 tggtgctgac aacggggata tcgtcctgca cgatctggtt ccattcgtcg ttataaccag
      721 tagcagtatt tggcgcctgg tacacgaaga ggtttgtcag cccattcaac gtgcccaaca
      781 ccacctccat gtccagttcg acttctatcg cgcccgatcc cggcgttgcg actccgccgt
      841 cgatcagcac gttgttgatg cgcgtcggcg agttcttgtc gtagcacgat tggaacgcgg
      901 caatgtcgct tcctgaatag ccatccagct cgaagagggc aatgccctgc ccttccccgc
      961 gcaagccggc gttataggcg cccgtgaaat tataggccga cgagaattgc gatggcagca
     1021 gatattccag cgcgtcactg ccgggaccgg ggcagacaat cgagcgtggc gagcgcgccg
     1081 gcgagatcga acgtggcgag cgcagcggcg gatgtgcgcg cgccgtcgcg ttatccaggc
     1141 cgttgatgtt ggcgatcagc ggtcgcaacg ccagcggcac gcgcggtgcg gcgctgttcg
     1201 cgtagaaaac tcgcccattc ccagcccggt atgtatgtat ctgtactccg aagctctgct
     1261 ctgcctgggc gaccgtcccg gaaaaatcga tcacctgctg gcctgccacc gcgcgcgtca
     1321 cgctaaagcc ttgcgcgcgc aaaaatcctg ccacctgctg cacatcctgc gcggtcggcc
     1381 catacagtgc cgcaaactca gcagcgctaa gatagcgatg aaacaggtac gaaccaggcg
     1441 tatattccgc ctgcacataa cgcgccaggt cagccgtgtt gcgcaaactc agattaatgc
     1501 tgagcgacag tcgcatcgat ggcaccgcat cgccgattgg cgcgctgcgt gccaccagtg
     1561 gcgaaaccgc cggaacgctt tctaacgcag ccatactcgc ccgcgtctga tacaacgaaa
     1621 agagcgagac gctgacgctg agcaacatca ccagtatgat aactggaaca agccacctgg
     1681 aatatttcat atcaccttcc tcacaagaaa acagtacgta ctatgttcag tatagtaagg
     1741 gtaccgaaat tatttctccc ccgccttctg tgatgcattg gtgatttttt tacctgtccc
     1801 tgtgatgtgg ttgtgactgg atgaatctac gattaaagcg aggaacggat tgggaagcag
     1861 tctaaagaga gcctcacatg agtatgagga tcaaggttca tatccatccg ttccaacagg
     1921 agtatgaagt catgagtatc gtcaagaatt tacggccacg ggccgcaacg caggttccgg
     1981 agcccccact cgcgaggttt ttgtttgctg atacacgtat ggcgtggttc tggttgattg
     2041 ttcgtgtgta cgttggctgg caatggctgg tagcaggcat ccacaaactg accggctatt
     2101 cccttgattt cagcacattt ggacagcata ccgggaattc ctggatcttc accgaccatc
     2161 taggagcacc gctgagcggc ttcctcaatg gcgtgatcac tccggccaac atcgcgaacc
     2221 cggcaacggg ctggtacgtc tggttcgtac agcatgtcgc gataccaggc gctggtgtct
     2281 tcacctacat ggtcaccttc ggtgaaatcc tagtcggact tggtttgatc ttcggggttt
     2341 tgaccggcat tgccgccttc ttcgggttgt ttatgaacct gaacttcctg cttgcgggcg
     2401 cgatcagcat caacccgatc attggtggcc tggccctgtt cgtcatgttg gcctggcgcg
     2461 tcgccggcta ctggggcatc gaccgctacc tgctgccgct gctcggcacc ccctggaccg
     2521 gcgcgctgac caggaaagcg caaaacatcc caccgacgcc tgccccggca taaaccggtc
     2581 aggtgtagag ggcaagaaga agcccctgcg agcggcgctc gcaggggctt cttcttgccc
     2641 tctacacttt ctcaactttc gcaaaggcgc tgccatcaag tgcatcacaa cgacgccagg
     2701 taatagccga cgccgacttt cgtcagcaga taacgcgggt gggtcggatc ggactcaatt
     2761 ttacggcgta cgcggttgat attgacctgc agcatatggc tctcacccat gtattccagt
     2821 ccccagacgt gttcgagcag caaatcctgc gtcacgatat gcccggcgtt ttgcgcgagg
     2881 taggccagga tacgatactc ggtgggcgtc atcggcacct ctttgccgtt caaggttacg
     2941 agatgctggg agtagtcgat gacgagatcg ccagttgagg cggtggccag caagccgcgc
     3001 gtgttttctt tggcggagaa ttccgagcgg cgcagcactg cgcgtacgcg cgccagcaac
     3061 tcatcgacgc tgaacggctt ggtcaggtaa tcgtccgccc ccaggtcgag gccgcgcact
     3121 ttgtcctggt cctgcccgcg cgccgtcacg agaataatcg gcaccgtaga aaattcgcgt
     3181 accttgtagc agaccgaaaa gccgtccatg cgtggcatca ttacatccaa caggacaagg
     3241 tcgggcgact tctgctcgat ttgctccaat gcctgctgcc catcgctgac cggcagcaca
     3301 tcgtaaccct ccaactggag gttgcgtgtc accaggcgca agagttgtgg gtcgtcatcg
     3361 gcagtcagta tggttgtttt cttgatggac atgtaattct tctcctctaa tatatacggc
     3421 tctctctccc cttctttgca acatacttga gatttattat aaaatagcta cgttcacatt
     3481 gaaatcacag aacatagcaa cacatcacaa atgtatcaca ttacgttgtt tggcctggat
     3541 aggcaaaaaa aacgatggca atattacacc cccgcaataa actgacgcgg cgagcagaga
     3601 ccgcgttgaa atcacagcga tttgcgcgaa tgcgcacgct attccacaca tggcgcgagc
     3661 taccctactc cggcgttgtg acgctgctgc tcagtaccct gctggcaact ctactcgtca
     3721 tgctgtttga ccgctcggtc gtcacactcg ttaatccggg gctgatctac ctgcccatta
     3781 tcgctatgat agcttatcac tggaaatggt acctggccgt catcggcgcg ctgctccaac
     3841 tcttctgcgt ctatttcttt tttctaccac cattcaactc cttcagtgta tccagcgagc
     3901 aggccattgc tcaactgatt gtcctggcag ctgctatggg ctttgtgctg gctatcgtgc
     3961 aacttgcccg ctatggccgc tccaacgcgg aacgcgctgc agctcgcttc gaggcgctca
     4021 accgcatcgg cgcggcactg acgagcgaac tggacgagtc gcgcctgctg cacactattg
     4081 ctgaaaccgc tcgcagcctg accaacgcag gttttgccgc ctttacgctg cgccccatca
     4141 acgaactcgg cgaaccgctg gtaccctcgg aaggccatct attccacctg gcggcagtgg
     4201 tcggcgtaac gaaagaacag gagcaactcc tccgccatat gccgctcggc ggcgaaggac
     4261 tgttagcgcc catcttccat cacggtatgg ccgtgcgtgt ccccgacgcg ctcgtctacc
     4321 tccagcagag cggaggcgcg catggcggca aagtacgcat gaccagcgag gatcgcttgt
     4381 tggcgcggca agctgccttc gactatgcgc acgggaatct agaccgcgac ggcttgcgct
     4441 ctatgggcgt accgcgcgga catcccatcg tgcgcagctt ccttggcgtt cccctgctcg
     4501 accgcgcggg caacgtgcgc ggcggcctgc tgctcggcca cagcgatcca gactgcttta
     4561 cattggcgga tgagcaactg ctggtcggcc tggccgcgca agccgctatc gccctggaaa
     4621 acgcgcggct ctacagcgcg gcgcagacgc aggcgcgcga actggatgcc atattcgaga
     4681 gcatcgctga tggtattatg ctcgtcgatg agcagggcga ggtactgcgc gagaaccgta
     4741 ccgcgcgtca tctacgcgag caagtggcat cgatgcaggc ggaccaaagc agccctacac
     4801 agcaaccgcg tgtggaagac ctgctgcgcg aggctgcgac gcgagcgcag gtcgacgcaa
     4861 ccgagccggg cggcataccg gcgaccatca ttgacaacgc gggagacgcg cgcgaattcg
     4921 tcatcaacgc ctcgcccttg cgtcctccat ctctcccgtt gctgtccgag caagacaacc
     4981 tggcgacaca caacgagtta aaagctatga acggctccgt cgtcgtctgg catgatgtca
     5041 cggaggcgca ccggctcttg atagagcagc aagctcatgc agagacggaa gcgcgccgcg
     5101 ccctcttgca aaccgtcatc gacgaactac caggcggagt atacctcgtg cgcgggcatg
     5161 atgcgcgtct cgtcctggcc aaccgcgcca tagccgatgt gtggggcgca acctggcaat
     5221 atgaccagcc tatgatcgaa ttcctcgaac agaacgacat ccgcctcttc cacttcgacg
     5281 ggcgttctct cacttcaggt gaactcgcca ccttgcgcgc cgtgcggcgc ggtgaatcga
     5341 tctaccacca gcaggaaatc atccggcata aagatggcac agccctgccc gtcctggtca
     5401 atgccgtcgc gctcgacccg ctcgtgcttg gctggaacgg cgagagtgtg ccagctgagt
     5461 ctgacccgga accggctgct atcgtcgttc accaggatgt gacggcgctg cgcgaggcgg
     5521 aacggctcaa ggatgaattc atcggcatcg ccgcgcacga actgcgcacc cccctggccg
     5581 tcatcaaagg cttcgcgcag atgttgattt cccagaccgc gcgcggcaaa ggtcccgcgc
     5641 tggccgactg gcaaagcgag gctatccagg atatcgacca ggctacgatc cgcctcgtgg
     5701 aactgaccga agacctgctc gatgtaacgc gcctgcaagg tggtcggctg gagctgcatc
     5761 ctgagccaac cgacctcgtg gcgctagcgc agcgcatcgc ggcgcgcgtc cgcgtgacaa
     5821 ccagcaagca cagcgtcaca attcataccg ggacagaaca tcttgtcgca accatcgacc
     5881 cccgtcgcat tgaacaggtc ctgagcaacc ttctcaacaa cgccatcaag tacagccccg
     5941 acggcggcga gatcaccata accctgcgcg aggataggcc aacctgctcc gccattctca
     6001 ccgtgcagga caatggcatt ggcatccccg ccggtcagca gggccgcatc tttggccgct
     6061 tcgcccgcgc cgacaacgcc cgcgactcca acatcagcgg caccggtctg ggcctctacc
     6121 tttgccgcga actggtcgag cgccacaacg gccgcatctg gttcgagtcc gtcgaaggcc
     6181 acggatcaac gttttatgta gcactgccat tagagaagtc cgcctgattc gtaagtcgag
     6241 gcatggcaac cgcgagggtt gccactacga taatgtgaga tacgctaacc cggcccgtta
     6301 tcgtagtggc aatccttgcg
//
LOCUS       DASZAK010001203         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_314290, whole genome shotgun sequence.
ACCESSION   DASZAK010001203 DASZAK010000000
VERSION     DASZAK010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314290"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..741
                     /locus_tag="VGT44_21180"
     CDS             10..741
                     /locus_tag="VGT44_21180"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV2583386.1"
                     /translation="MKQSLPTYPPPLADEPVIFTLAGMMHGARKALPLAASVFFVGLV
                     FGVLARQARMGGVEATLMSVLVFAGASQFVALSLWMIAPFPVMTIILTTFVVNMRHLL
                     MGASLRPWFSRLPRLAAYCTLFFMVDESWALTMGELNAGGRDGAFLLGSGLLLYVAWV
                     ASSIVGLVAGASIQNPAQWGLDFAFTAVFIALLAGMWRGRSQFLPWAIAAGAALAAYR
                     WLPGEWYIVLGGLAGSITGAIFYAR"
     gene            731..1033
                     /locus_tag="VGT44_21185"
     CDS             731..1033
                     /locus_tag="VGT44_21185"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV2583387.1"
                     /translation="MPGEIIVVMTIIGMALVTYAARAGGLWLMGFVTPSARVEAWLRQ
                     IPGAVLIAIIAPSVFLTSLAQTLAALATLLAAIYTKNILVAMLVGVVAVWLLRKAL"
     gene            complement(1036..3288)
                     /locus_tag="VGT44_21190"
     CDS             complement(1036..3288)
                     /locus_tag="VGT44_21190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2583388.1"
                     /translation="MSIALRHIGKYELYQRLASRKLNETWKAFDTQARRYVKINIFYT
                     TFPADSEQIAQFRQQAEKVAALHRPDIERIYDTLVFPSKRVDSPVASMVCLISEYIEG
                     STLADYIHHTQSIGKSRPGSDCVQFFTTLSQAIDDAHGQGIMHGNIQPANIVLRGSNS
                     QSGQLEEPVLTDFDFIKLLRDHSIANNSLYLSPEQIKGQPATKRSDTYSLSVILYELC
                     TGVLPFRGNRPVAIMMQHLTTPPTPPALLNPTIPASLTQVIMRGLTKEPERRFASASS
                     LTIALARSLSLPIPEELNRPAFLRDALQSPDDGDTLQPGRQTGTLPATSGVTSSHWTT
                     SRKGVQFDTMPSREANNRQRKRLPGPRYTIALIILLLATLSTIGAALLFSQNKPPGAA
                     DQLVGHAFFLNSGQINADTAQGINDELQIDLSGIPDPPAGKSYFAWLLVDRSVSESLP
                     VLLGTLRIDQGNVHFLYHGDGQHSNLLATTSRFLVTLDDTQHPTSNPLLDTSTWRYYA
                     EIPSIPSPTDALHFSMLNHLRHLLVESPELQIRGLHGGLAFWLARNISTISASANDAR
                     DAWHNQDTKTIHTRLVQILDLLDGVLFARSDLPPGTPLLADAHSVQIPLLGPAPNAPD
                     PPGYAYSGEVPPGYVYLISEHMSGAIQSPQTTPDQKQLAIKINTGLDSVKSLLAQIHQ
                     DAIALLKMDNSQLLQPASLSQLDNLAIEAQIAYTGQLSPSTGQSESGALWIYGNLQRL
                     AAFDVRPYLQ"
     gene            3476..>3669
                     /locus_tag="VGT44_21195"
     CDS             3476..>3669
                     /locus_tag="VGT44_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583389.1"
                     /translation="MEQRAENVGHKLKPLVTREARWWTWRKVVVATALVLVILETGSY
                     AFNWTWTGFKDNNTVWDWLQ"
BASE COUNT          722 a          905 c         1134 g          908 t
ORIGIN      
        1 taagtattca tgaaacagtc gcttccgacg tatccgccgc cgttagcaga tgagccggta
       61 atcttcacgc tggcaggcat gatgcatggc gcacgtaaag cgcttccact tgctgccagc
      121 gtcttttttg tcggactggt cttcggggtg ctggcgcggc aggcaaggat gggcggagtt
      181 gaagcgacct tgatgagcgt gcttgttttc gccggagctt cgcagttcgt cgcgctctct
      241 ttatggatga ttgcgccatt tccggtgatg accattattc tgacgacctt cgtcgtcaat
      301 atgcgacacc tgctgatggg cgcttcgctg cgtccctggt tctcgcgtct tcctcgcctg
      361 gccgcgtact gcacgttgtt cttcatggtt gatgagagct gggccttgac aatgggcgag
      421 ttgaacgcgg gcggacgtga cggggccttc ctgctcggca gcggcctgtt gctttatgta
      481 gcctgggtcg cttcgtctat cgtcgggctg gtcgcggggg cgtccatcca gaatcccgcg
      541 cagtggggac tggattttgc cttcaccgcc gtctttatcg cgctgctggc ggggatgtgg
      601 cgcggaaggt cgcagttcct gccctgggct atcgcggcag gagcagcgtt ggccgcatat
      661 cgctggctgc cgggtgagtg gtatatcgtg ctagggggcc tggcgggcag catcacggga
      721 gcgatttttt atgccaggtg aaatcatcgt cgtgatgacc attatcggga tggcgctcgt
      781 cacctacgcc gcacgcgcgg gtggtctctg gctgatgggc tttgttacgc cttctgcgcg
      841 cgttgaggcc tggctgcgcc agattccagg tgcggtcttg atcgcaatta ttgccccttc
      901 ggtcttcctc accagccttg cgcagacgct ggccgcgctc gctaccctgc tggccgcgat
      961 ctatacgaag aatatcctgg tcgcgatgct tgtcggcgtg gtggcggtct ggctcctgcg
     1021 caaggcgctt tagcgttatt gtaaatatgg tcgcacgtca aaggcagcca ggcgctgcag
     1081 gttgccgtag atccagagtg cgccgctttc cgactggcca gtcgaggggc taagttgacc
     1141 ggtgtaggca atctgagcct ctatcgccag gttgtcgagc tgcgaaagtg aggcgggttg
     1201 caagagctgc gagttgtcca tcttcaggag cgctattgca tcctggtgaa tctgcgctaa
     1261 caaacttttc acgctgtcca gtcccgtgtt aatttttatg gcgagctgct tctgatctgg
     1321 cgttgtctgg ggagactgaa tcgccccact catatgctcg ctgatgagat agacataccc
     1381 aggcggtact tcaccgctgt aggcataccc aggcgggtct ggagcgttcg gggctggacc
     1441 gagcaaggga atttgcacac tgtgtgcgtc ggcgagcaat ggtgtgccgg gagggaggtc
     1501 gctgcgcgcg aacaacaccc cgtcgagaag atcaagaatt tgaacgagcc gggtgtgaat
     1561 cgtcttcgta tcctggttgt gccaggcgtc acgcgcgtcg ttggcagagg cggaaatagt
     1621 cgaaatgttt ctggctagcc agaaagccag tcctccatgt aatccacgaa tctgtaattc
     1681 gggtgactca accagcaggt gacgcaggtg attgagcata ctgaaatgta gtgcatcggt
     1741 aggactggga atggagggaa tctcggcgta atagcgccag gtgctggtat caagcagtgg
     1801 attgctggtg gggtgttgtg tatcatcgag cgtgaccagg aagcgacttg tcgttgccag
     1861 taaattgctg tgctgtccgt ctccatgata gaggaaatgt acgttgccct gatcgatgcg
     1921 taacgttcca agcagcaccg gcagcgattc gctcacgctt cgatcgacaa gtaaccaggc
     1981 aaaatagctt ttcccggctg gcggatcggg aatgccggag aggtcgatct ggagttcgtc
     2041 attgatgcct tgcgctgtat cggcgttgat ttgcccgcta ttgagaaaaa aggcatgacc
     2101 caccagttgg tccgcggcgc cgggcggctt attctgtgaa aagagcagag ctgctccgat
     2161 ggtactcagc gtggcaagca gtaagataat cagcgcgatc gtgtatcttg ggccgggcaa
     2221 tcgtttcctt tggcgattgt tggcctccct cgaaggcatc gtgtcaaact gcaccccctt
     2281 cctgctggtt gtccagtgcg atgaggttac tccagaagta gccgggagag ttcctgtctg
     2341 ccttcctggt tggagcgtgt cgccatcgtc cgggctttgt agggcatctc ggaggaaggc
     2401 gggccggttc agttcttcag gaataggcaa actgagcgag cgcgccagcg ctatcgtgag
     2461 cgaggaggcg ctggcgaatc gtctttctgg ctctttggta aggccgcgca taatgacctg
     2521 tgtcaaggag gccgggatcg tcggattcag caaggcagga ggcgttggcg gcgtcgtgag
     2581 gtgctgcatc atgatggcga cgggcctgtt tccgcggaaa ggcaagacgc cggtacagag
     2641 ttcatagagt atgacgctga gcgagtaggt atcgctgcgt ttggtggcag gttgtccttt
     2701 gatttgttct ggcgaaagat agagcgagtt attcgcgatg ctgtgatcgc ggagcagttt
     2761 gatgaaatca aaatccgtca ataccggctc ttccagttgg cctgattggg aattgctccc
     2821 acgcagcacg atattcgccg gttggatgtt accgtgcatg ataccctgac cgtgggcatc
     2881 gtcgatggcc tggctgagag tagtgaaaaa ctggacgcaa tccgaaccgg ggcggctttt
     2941 gccgatgctc tgggtatgat ggatgtagtc tgccagggtt gaaccttcga tgtattcgga
     3001 gatgagacat accatcgagg ctaccgggct atctacccgt ttggaaggaa agacaagcgt
     3061 atcgtaaatc cgctcaatgt caggtcgatg aagggcggcg actttttcag cttgctgccg
     3121 aaattgtgct atctgctcag aatccgccgg gaacgtcgta tagaagatgt tgattttcac
     3181 gtaacgccgt gcctgggtat cgaaagcctt ccacgtctcg ttcaatttac gactggccag
     3241 gcgctgataa agttcgtact tcccgatatg acgcaaagct atactcatgc tcatcctctt
     3301 attcattttt ccaaacctta ttacgagaac gggttacagc ttgacagaat tacgagcaat
     3361 tctttatgct gttatcagcg gataatgtca tgtagcgcct ttatctcatc atttcgaccg
     3421 gctcaaaggt ttgaaacaat tgtgcgaccc tcactgaaaa caaaaggagc gcagcgtgga
     3481 acaaagagct gagaatgtgg gccataagct caaacctctt gttactcgcg aggcgagatg
     3541 gtggacatgg aggaaagtag ttgtagcaac tgcgctagtt ctggtgattt tggaaacggg
     3601 gtcctatgcc ttcaactgga cctggacggg cttcaaggac aataatacgg tctgggactg
     3661 gctgcaatt
//
LOCUS       DASZAK010001204         6599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_636386, whole genome shotgun sequence.
ACCESSION   DASZAK010001204 DASZAK010000000
VERSION     DASZAK010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636386"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VGT44_21200"
     CDS             complement(<1..232)
                     /locus_tag="VGT44_21200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583390.1"
                     /translation="MNEILSDAAYFRQQQALRDEAAQRGLSGFAISATHESITARMQA
                     GAERVLRLIEEGKLEEALVILNTDDWCLEEKEI"
     gene            complement(366..1763)
                     /locus_tag="VGT44_21205"
     CDS             complement(366..1763)
                     /locus_tag="VGT44_21205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583391.1"
                     /translation="MATTNNVPSRMMSTVIFSSTVGTAIEWYDFFLYGTMATLVFPKV
                     FFPASNQLVGTILSLFTFLVGFIARPFGGALFGHMGDRVGRKSTLITTLLLMGISTLL
                     IGFLPGYATLGVAASLILVLLRLCQGLGVGGEWGGSVLLAMEYGHRRNRGFWASWPQM
                     GVPVGLILSTVIVNIVQISAGTSFVTGFAWRIPFFISAALVLIGLYIRLRVLETPLFE
                     EVKNQRREVSAPIIEAFRHSTPEILLSAGARFVEQAPFYLFTTFVITYGVDKLALGRT
                     DILNAITLAAIVELFTIPLFGYLSDRVGRRRWYLVGCVLMAAFAFPYFLLLNSGSIAI
                     FTLSVVLSVAIFHAWVYGPQAALIAERFGTRSRYSGASLGYQLAAPFAGGLAPIIGVL
                     LLNGKSSLSALGLSKVVVNIGGGSWQAVSIYMIILAVISFASVLGLKELTRADISNVD
                     AYKITEEQIVLAPTD"
     gene            complement(1934..3274)
                     /locus_tag="VGT44_21210"
     CDS             complement(1934..3274)
                     /locus_tag="VGT44_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583392.1"
                     /translation="MATLHLEKTEESPPREQILPVIVASALGSAIEWSDFFYYSFLAV
                     TVFPTVFFSPLNPSIGILASFTANFIGFVARPLGGVLFGRIGDRVGRKSTLAATLLLI
                     GVTTMFMGVLPSYAQLGIAAPLLLVSLRFLQGVGVGGEWGGAVLLTMEFSNQRRRGFW
                     TSWPQIGVPVGLALSAASILLFKSLYPGEAFQVIGWRIPFLLSTILVALGLYIRLRIP
                     ETPAFLRLVASHRQAVNVTLTACRLYWREILLTALIRTGEQAPYYIFTTFALTYGTLI
                     LHLDVTLIFISMIAASGVSSCMMPCFGALSDRVGRRRTTIYGALMMMVCAFPYFLLFN
                     TRNPLLVILALALALGCPHACLYGPQSSLIAERFPTRLRYTGASLGYQLASIIAGGPA
                     PIIATYLLTRSSAQPLALPAWVLIAVYIIAAALITLLAALPLKEYAGRAAREDG"
     gene            3464..5461
                     /locus_tag="VGT44_21215"
     CDS             3464..5461
                     /locus_tag="VGT44_21215"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583393.1"
                     /translation="MLRILERKIALQLLVFYGLFVIPLLLGGAELYFFQYGALQQSAE
                     QADLGLAEAIAQNVELQAQGTAKGTIDPAQLNARLIAEQQQLTTAGEGRIWILDSNGD
                     LIADTVGIPPHSNLLQRIPGLRAALQGKPGSVVAQDQQRDWLYSYVPITGTNWAVAVG
                     RPTDVTFAAVISFQHSLIIALIMLLAGASIFWFVMHGWMVAPLGHLARAVAMMRPDQT
                     VKVMDGKQLTREKRRQDEIGRLVNAFATMEEEIHTLFRKSDEQSFSRLQTLDAIMRSM
                     EEGVLLETPAGQIVYANHSFMQFVGFSTRDRQPDSLQESDLTERMESLMEDLKAYRES
                     LHSAASGEGPQVIEFQVRGYYNQLGQLVPSRRYLRMRLFQVYDMANQLIGRGKIYNDV
                     TQQSEAEKVKKNLLAIVSHELRTPLTAIKGYATSLLETDVELDATTQRDFLQRIVEEE
                     DRMAELVTSLLEMSQLEAGTLKLAPGYCRLDTLIQRAIEAAQCEQLCVTLSLPAEAPV
                     LYVDQRRMEMVLRNILENAQRYAVGAGDPGGETEIEITARYALENEDQGVYLSIVDNG
                     PGLPGDLTERIFERFYQVDGGRLRSSSGVGLGLSICRGFVEAHGGRIWAENRTGNENN
                     HGNSGHESNDGSGAAFHIWLPSRLLYVPGSRRVPFELEKAL"
     gene            5486..6178
                     /locus_tag="VGT44_21220"
     CDS             5486..6178
                     /locus_tag="VGT44_21220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583394.1"
                     /translation="MAQEKHILVVDDEPSIQHFVKRNLELRAFKVSLASNGLEALAQF
                     EHANFALIILDIMMPSLDGLEVCRRIRQHSTVPIIILTALGEESDKIAALDQGADDYL
                     TKPFGVGELLARVKAVLRRNHWADQPVSIGTTRFGDLEIDFEQRRVWKEGTLIKLTPT
                     EFELIQELALHPGKIFTHEALLRRVWGTEYRSESEYLRVYIGRLRRKLENDPARPAHI
                     LTEPGVGYYMVK"
     gene            6404..>6599
                     /locus_tag="VGT44_21225"
     CDS             6404..>6599
                     /locus_tag="VGT44_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583395.1"
                     /translation="MQQPRPRSQAFALFGTYALEDIPKTYSWFEEMRLHHPVFQDERT
                     RLWQVFRHEDVLAVLTDYTRF"
BASE COUNT         1619 a         1815 c         1918 g         1247 t
ORIGIN      
        1 tgatttcctt ttcctccagg caccaatcat ctgtatttaa aataaccagg gcttcttcaa
       61 gtttgccctc ctcaatgaga cgcaggacgc gttccgcacc tgcctgcatg cgtgctgtaa
      121 tcgactcgtg cgttgcagag atggcaaatc ccgataggcc tcgctgcgct gcttcatctc
      181 ttaaggcctg ttgttgtcta aaatatgctg catcactcag tatctcgttc atactttcaa
      241 ctcaagccag cgaaccgtcc gcctgggtca ggaattatct gtaggtaagc acccagacag
      301 aaatggtaat gaaacccgta ggaccgataa agcggcccta cgagtacgat tgggtgagac
      361 gtatctcaat cggtgggcgc caacacgatc tgctcctcgg tgatcttgta cgcatcaacg
      421 ttcgagatgt cggccctcgt caattctttc agacccagca ccgaggcgaa cgagatgacc
      481 gccagtatga tcatgtagat cgaaaccgcc tgccaggaac ccccgccgat attgacaacg
      541 actttgctca ggcccagggc gctcaggctg cttttgccgt tgagcaacag taccccgatg
      601 atcggggcca ggccacccgc gaagggcgct gcaagctggt agcccagcga tgccccgcta
      661 tagcgcgacc gcgtcccgaa ccgctcggcg atcagcgcgg cctgcgggcc atacacccag
      721 gcgtggaaga tggcgaccga aaggacgacc gacagggtga agatggctat tgatccggaa
      781 ttcaacagca agaagtaggg gaaagcaaac gccgccatca gaacgcagcc aaccaggtac
      841 catctcctcc tccccacccg gtcggacaaa tagccgaaaa gcgggattgt gaagagttcc
      901 acgatggcag cgagggtgat ggcattcagg atatccgttc taccaagcgc cagtttgtct
      961 accccgtagg taatcacaaa cgtagtgaag aggtagaatg gggcctgctc gacgaagcgc
     1021 gcgccggcgc tgagcaaaat ctcaggggta ctgtgccgga atgcctcgat gatcggcgcg
     1081 gaaacttcgc gccgctgatt tttcacctcc tcaaagagcg gcgtctccag cactctcagg
     1141 cggatataga gcccgataag caccaaagcc gcgctgatga agaacgggat gcgccacgcg
     1201 aagccagtga caaaactcgt accagcactg atctggacaa tgttgacgat cacggtcgag
     1261 agaattagcc cgaccggcac tcccatctgc ggccagcttg cccagaagcc gcgattacgc
     1321 ctgtggccat actccatagc gagtaggacc gagccgcccc attcgccgcc gacgcccaga
     1381 ccctgacaca ggcgcaggag gacgaggatc agggacgcgg cgacaccgag ggtagcatag
     1441 ccaggcagga aaccaatcag gagcgtcgag atgcccatta acaggagcgt agtaatcagt
     1501 gtggatttgc ggccaacacg gtcacccata tgcccgaaga gcgcgccgcc gaatggccgg
     1561 gcgatgaagc caaccaggaa ggtgaacaat gacaggatcg tgcctacgag ctggtttgac
     1621 gcggggaaga aaacctttgg gaagaccagg gtagccatcg tgccgtacag gaagaagtcg
     1681 taccactcga tggctgtacc aacagtgctt gagaagatca ccgttgacat catacgtgat
     1741 gggacattat tcgttgttgc catgtgttcc tccagtttgt gttaatgacg cagagatgta
     1801 ctacaacaag gcagggaata gcgcgacaag gacaatcctg ggtgtgtgca tgctacacac
     1861 gaaaagtata gaaggcaggg ataaatatca cgtaaacaaa gcggagaaga tgtaaacaat
     1921 tctgtaaata aattcagcca tcttccctgg cggcgcgccc ggcatattcc ttcaaaggca
     1981 gagctgccag cagcgtgatg agcgcggcgg cgatgatgta cacggcgatc aagacccagg
     2041 ccggcagtgc caaaggttga gcggatgagc gagtaaggag gtaggtggca ataatcgggg
     2101 cagggccgcc agcgatgatc gaggccagtt gatagcccag cgatgcaccg gtataacgaa
     2161 gccgtgtagg aaagcgctct gcgatcaagg aagattgagg cccatacagg catgcgtggg
     2221 gacatccgag agcgagcgcg agcgccagga tgactagcag tggatttctg gtgttgaaca
     2281 acaggaaata cggaaaggcg cacaccatca tcatcagcgc gccatagata gtggtgcgcc
     2341 tccgcccaac ccggtcggag agcgcgccga aacacggcat catgcaggac gagacgccag
     2401 aagcagcgat catgctgatg aagatcagcg tcacatccag gtgcagaatc agcgtgccat
     2461 acgtgagcgc gaaggtcgta aagatgtaat agggcgcctg ctcgccggtc cgtatcagag
     2521 cagtcaggag gatttcacgc cagtagaggc ggcaggcggt cagggtgacg ttgaccgcct
     2581 gccggtgaga ggcgacaagg cgcagaaacg cgggcgtttc gggaatgcgc aggcgaatat
     2641 agaggccgag tgcaaccagt atagtactca gcaggaacgg gatgcgccag ccaatgacct
     2701 ggaacgcctc accgggatag aggctcttga agagcaggat cgaagcggcg gagagcgcca
     2761 ggccgaccgg cacgccgatc tgtggccagc ttgtccagaa gccccgccgg cgctggttgc
     2821 taaactccat cgtcagcagc acggccccgc cccattcgcc tcctacgccg acaccttgca
     2881 gaaagcgcag cgataccagc agcagcggcg cggcgatgcc cagctgcgcg tagctgggca
     2941 gcacacccat gaacatcgtg gtaacgccga tcagtaggag cgtcgccgca agggttgact
     3001 tgcgcccgac gcggtcgccg atgcgaccaa ataagacgcc gcccaggggc cgggccacga
     3061 agccgatgaa gttcgcggta aacgaggcta atatgccgat cgaaggatta aggggcgaaa
     3121 agaagacggt ggggaagact gtcacggcca gaaagctgta gtagaagaaa tcagaccact
     3181 cgatggccga gccgagcgcg ctggccacga tgactggtaa gatttgctcg cgcggggggg
     3241 attcttccgt tttttctaga tgcagcgttg ccattgatct tcctccagca ctaagcatag
     3301 cccatgacgt ataaaaaagc tataaacgga aacggcaggg gtgtaaacaa acatataaat
     3361 atttcttgta gtgatgaggc tgcatcctct cacctattga attactcacc agattaccac
     3421 atactataca tgatgtcgtt gcctcgcgga ggagaagctt gcgatgctca ggatactaga
     3481 gcgcaaaata gccctgcagt tgctggtctt ttatggcctg tttgtcattc cccttctgct
     3541 gggcggcgcg gaactctatt ttttccagta tggcgcgcta cagcagagtg cagagcaggc
     3601 cgaccttggg ctggcagagg ccatcgcgca gaatgtcgag ttgcaggcgc agggcacggc
     3661 gaaagggact atcgacccgg cgcaactgaa cgcgcgcctg atcgccgagc agcaacagtt
     3721 gaccaccgcc ggcgagggac gcatttggat actggatagt aacggtgatc tcatcgcgga
     3781 tacagtgggg attccgcctc atagcaatct gttgcagcgc attcccggcc tgcgcgccgc
     3841 actgcaaggc aaacctggca gcgtggttgc gcaggatcag caacgcgact ggctctatag
     3901 ctatgtgcct atcaccggca caaactgggc cgtggctgtg gggcgtccaa ccgatgtcac
     3961 tttcgccgcc gttatcagct tccagcacag cctgattatc gcgctgatta tgctgctggc
     4021 cggtgccagc atcttctggt ttgtgatgca cggctggatg gttgccccgc ttggccacct
     4081 ggcgcgggcc gtcgcgatga tgaggccgga tcagaccgtg aaggtcatgg acggcaagca
     4141 gttgaccagg gagaagagac gccaggacga gataggtcgc ctggtgaacg ccttcgcaac
     4201 gatggaggag gagattcaca ccctcttccg taagagcgac gaacagagct tttcgcgcct
     4261 gcaaaccctg gacgcgatca tgcgtagcat ggaagagggt gtcctgctgg aaactcctgc
     4321 cgggcaaatc gtctacgcca accacagctt catgcaattt gtgggcttct caacgcgaga
     4381 caggcaacct gactccttac aagaatccga cctgaccgag cgcatggagt ctttgatgga
     4441 agaccttaaa gcttatcgcg agtcgttgca tagcgctgca agtggcgaag ggccgcaggt
     4501 gatcgagttc caggtgcgcg ggtactacaa ccagcttggt cagctcgtcc catccaggcg
     4561 gtacctccga atgcgtctct tccaggtgta cgacatggcg aaccagttga tcgggcgcgg
     4621 caagatttac aacgatgtga cgcagcaaag cgaagccgaa aaggtcaaaa agaacctgct
     4681 agcaattgtc tcacacgagc tgcgcacgcc gctgactgcc atcaaaggct acgccaccag
     4741 cctgctggag accgacgtgg aactggacgc aaccacgcag cgcgactttt tgcagcgcat
     4801 cgtcgaagaa gaagatcgca tggccgagct tgtcaccagc ctgctggaga tgtcacagct
     4861 cgaagcagga acgctgaaac tagcccccgg ctactgccgc ctggataccc tgatccagcg
     4921 cgccatcgag gcggcgcaat gcgaacaatt gtgcgtcact ctctccctcc cggcagaagc
     4981 gcccgtgtta tatgttgatc agcgccgcat ggagatggtg ctgcgcaata tcctggagaa
     5041 cgcgcagcgc tatgcagtcg gcgccggaga tccgggcggc gaaacagaga tagagataac
     5101 cgctcgctac gcgctggaaa acgaggacca gggcgtttac ctgagcatcg tggacaatgg
     5161 gccgggccta ccaggagacc tgaccgagcg catcttcgag cgtttttacc aggtcgatgg
     5221 cgggaggttg cgcagcagta gtggcgtggg tctgggtctc tcgatttgcc ggggctttgt
     5281 cgaggcacat ggtgggcgca tttgggcgga aaatagaaca gggaacgaaa acaaccacgg
     5341 taacagcggc catgagagta acgatggcag cggggcagca ttccatatat ggcttcccag
     5401 cagactgctt tatgttcccg gttcgcggcg cgtaccgttc gagttagaga aggcgctgta
     5461 aattaatgaa gaggtatgtc agcgtatggc acaagaaaag cacattctcg tcgtcgatga
     5521 cgagccgagt atccagcatt tcgttaaacg aaatttagaa ttgcgcgcct tcaaggtctc
     5581 gctggccagc aatggactgg aggcgctggc tcaatttgag cacgccaact tcgcgctaat
     5641 catcctcgac atcatgatgc ccagcctgga tggcctggag gtctgccgca gaatacgcca
     5701 gcacagcacc gtccctatta tcatcctcac cgcgctagga gaagagtccg acaagatcgc
     5761 cgcgctcgac cagggagccg acgattacct gaccaagccc ttcggcgtcg gcgaactgct
     5821 ggcgcgtgtc aaggccgtac tgcggcgcaa ccactgggcg gatcagcctg tttcgattgg
     5881 cacaacgcgt ttcggcgacc ttgaaatcga cttcgagcag cggcgcgtct ggaaagaggg
     5941 cacactgatc aaacttacgc ccacggaatt tgagctcatc caggagcttg cgctgcatcc
     6001 tggcaagatt ttcacccacg aagccctgct caggcgcgtc tggggaacag aataccgcag
     6061 cgaatctgaa tacctgcgcg tctacatcgg tcgcctgcgg cgcaaactcg aaaacgatcc
     6121 cgcccggccc gcgcatattt tgacggaacc gggagtaggt tactatatgg tcaagtgaac
     6181 ctcaactatc ctggccactt agcatgccca ttcgaaaaca agcttcttct tttagctgta
     6241 ccaacttgat tgcaactttc aatccgatat atcgtaaatg aaaaatagca agacatatcg
     6301 cactggttca tttggtaaac gcgattatag gacgcgaatt ctcgcgtccc gtcctacatt
     6361 ttgcaagaag ttttcgcaaa aagtgaaaag aaaggcatgt tgaatgcaac aaccgagacc
     6421 tcgttcacaa gcgttcgctt tatttggtac ctacgcgctc gaagatatcc cgaaaaccta
     6481 ctcctggttt gaggagatgc gtctacatca tccagtgttc caggacgaga gaacgcgcct
     6541 gtggcaggta tttcgtcatg aagacgtgct agcagtactg accgattata ctcgctttt
//
LOCUS       DASZAK010001205         6762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_349970, whole genome shotgun sequence.
ACCESSION   DASZAK010001205 DASZAK010000000
VERSION     DASZAK010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6762
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349970"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..588)
                     /locus_tag="VGT44_21230"
     CDS             complement(154..588)
                     /locus_tag="VGT44_21230"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV2583396.1"
                     /translation="MGKKSIVWRLGRLGRSLKDLIETVQALEQCSIELRSLKENIDTT
                     TPTGKLMFHMTAALAEFERDVIRERTWLDWTPHVSVDTQEADAKPARCCALSNWNAQN
                     NCTLPGRTALLKLWPLLASRAALRSINMSSMAQTLRGPQVEV"
     gene            494..1003
                     /locus_tag="VGT44_21235"
     CDS             494..1003
                     /locus_tag="VGT44_21235"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2583397.1"
                     /translation="MLHCSRACTVSIRSLSERPRRPSLQTIDFFPIGKATGIDVWKEA
                     EQSGNALWVTEHNAKLEGVAERVVLHTADMRHLPFFDASFDLVVSSMAIHNIPTPSGR
                     KQALHEAVRVLKPGGKLVIVDFRQTGHYEQHLRTLGMDRITHHPLGWRFWYVGPWTAP
                     KLVRALKPA"
     gene            complement(1354..3984)
                     /locus_tag="VGT44_21240"
     CDS             complement(1354..3984)
                     /locus_tag="VGT44_21240"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2583398.1"
                     /translation="MQPDHPFGAWLRQQRKALDLTQAELAQRVGCAPVTMKKIEAGVR
                     RPSKQIAERLAAVLAIQPPLQAAFVAFARGLSAVPPTMASPGAGPVHHVPSQPTAFVG
                     RSRELAYLQRLLDAPDSRLMTIVGPGGSGKTRLAVEAAHRNRTSFAHGAHFVSLAAVT
                     SADAWLFAIADVLEYSLVGHEAPAVQLAASLRDKTVLLLLDNVEHVLDGVPVLVDLLA
                     GAPHLKLLITSRERLNLHGEWVLPLDGLDYPLTADADDDHLASYSAVQLFVQSAQRVQ
                     PDFALQGMAPDVLRICQQVEGMPLALELAAAWVRLLPCSHIAEQLAASLDFLASPLRD
                     VPERHRSLRAVFDQAWNVLSPPEQAALARLSVFRGPFDLEAAREIGGASLPVLASLAD
                     KSLLQVSGPGWYALHELLRRYAAAHLLYQGVEAGHAERAIDYLSQAAQRAGRAAAQRQ
                     AAGLLGQAVAIAEEAGLSDLASSLHHKRGQALLEACLWVEARPAFAAALAAAGLEQLD
                     RQVQLLLASAEVDFYLHDVAAQRHHVSEALRQAEEAQRADLVVAAMVKLGDVETNVGH
                     LTEAVSRYERALAGGGDAHHHLGRTYYWLGRYPEALTSLRAAVAVRQGQPLKQIFPLQ
                     DLGLALVATGQYTEAVRAFGEAQHLSREHEVWPLLARAVANLAGLHLEVFDYAGHEAL
                     AEEARALARSADFVLAEVSAGLDLLFNYARRNEVGRAAHLLADVAATVEKAHGSHGWL
                     WRLRLAQARAELALAQQEWEETLRLADLAMQHSRQAGRVKYLALALKTRAQALVARGH
                     SREALSDLRKALDLTGPTSDPGVFLQIAASLLAVEGDDALAREAYAVVQRLSAALPDD
                     EMRQSFAAAEPVRQLVRWMG"
     gene            4283..4609
                     /locus_tag="VGT44_21245"
     CDS             4283..4609
                     /locus_tag="VGT44_21245"
                     /inference="COORDINATES: protein motif:HMM:NF020382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhR family protein"
                     /protein_id="HEV2583399.1"
                     /translation="MTKTILQINYRFNASRADHTALVTPMAAPIAAVPGLLWKVWLMN
                     ENEQEAGGMYLFESGEAAQAFVSSPPITSFAAHPSLSSFSVKLFEAVESLSKITRGPL
                     LVAETV"
     gene            5817..6347
                     /locus_tag="VGT44_21250"
     CDS             5817..6347
                     /locus_tag="VGT44_21250"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEV2583400.1"
                     /translation="MTVLTTGDTAVHLLHFMAPRTGQSSGTGSIVVDAVISVLFFLIG
                     LFSLYVSVRGGLNVFNLSRNSVVVKGSITAHRSRSTGAGGRVVRAVGEQYLATYRYEY
                     GGKTYSHEQAVGGIPLVGEAVSVRCVAHHPNVALLEGPASASLIKERKVALVVMPLLA
                     ATFLGVAIFEALQLLR"
BASE COUNT         1370 a         2036 c         1989 g         1367 t
ORIGIN      
        1 agaaccgtgt ggaagcgaat ttggcaagtg gtgtttggta taccagatgt gatatcttat
       61 agccgtacca cctccgatgc gcgcgagcat tgtgcataag ctcacttgtc atttcaaaaa
      121 ttaaatgacg ctgaatttga atatttattt ggctcacacc tctacttgtg gacctctaag
      181 ggtttgagcc attgacgaca tatttataga acgtagcgcg gctcttgaag ccagtaaggg
      241 ccataatttc agcaacgctg ttctgccggg cagcgtacag ttgttttgcg cgttccagtt
      301 gctcagggcg cagcatctcg ctggctttgc gtcggcctcc tgtgtgtcca cggatacgtg
      361 cggcgtccag tccagccagg tgcgctcgcg gatcacatcg cgctcgaact cggccagagc
      421 agctgtcatg tggaacatca acttgccggt gggcgtggta gtgtcgatat tctccttcag
      481 gcttctcagc tcaatgctgc actgctccag ggcctgcacg gtttcaatca ggtctttgag
      541 tgagcgtccg aggcggccga gcctccagac aatagacttc ttccccatag ggaaggcaac
      601 cgggattgac gtgtggaagg aggcagaaca atctggcaac gctctgtggg tgacagaaca
      661 caatgccaaa ctggaaggcg ttgcagagcg ggttgtcctg cacacagcag acatgaggca
      721 tcttcctttt tttgacgctt ccttcgatct ggtcgtctca agtatggcta tccacaatat
      781 cccaacgcct tcaggtcgca aacaggctct tcacgaagca gtgcgtgtcc tcaagcccgg
      841 cggcaaactc gtgattgtcg atttccgcca aacaggacac tatgagcaac atctgcgcac
      901 attgggaatg gatcgtatca ctcatcaccc acttggctgg cgcttctggt acgttggtcc
      961 ctggacggct ccaaagctgg tgagggcgct caaacctgca tgaggggtcc gttggtcgag
     1021 gatgacgatg caagaggagc attcatcgat gtcatgatct caattccaag atagccatcg
     1081 agagaaatcg gggtatgtac aactttctct catcgtcctc tagacgagga gtggtttttc
     1141 cgggcatcga tgcgtcaagt tccatgattc agtgcaactt cctaccctta cgtgcaaaat
     1201 cggcccaccc ttcagtgaat ttaccatcaa cagcataatt accaaaattt cgccatcata
     1261 gctcgcaatt ttacaccgtg ggagttccac tttctgcttc acctctcatt caggcagcaa
     1321 agaaacacac tggaatgaag cggaggccgc cggttacccc atccacctga ccagttgtcg
     1381 gactggttct gcagccgcaa agctctggcg catctcgtca tctggaagcg cggcactcag
     1441 gcgttgtacc accgcgtagg cctcccgcgc cagagcatcg tcaccttcca cggcgagcaa
     1501 gctggcagct atttgaagga acactcccgg atcggaggtg ggtccggtga gatccagggc
     1561 cttcctcaga tcgctcagcg cctcacgcga gtgtccacgt gcgaccaggg cctgcgcgcg
     1621 cgttttgagg gccagggcca gatatttgac gcgaccggcc tggcggctgt gctgcatcgc
     1681 caggtccgcc aggcgcaggg tttcctccca ctcctgctgc gcgagtgcca gctcggcacg
     1741 ggcctgcgcc agccgcagcc gccagagcca gccatgactg ccatgcgcct tttccaccgt
     1801 tgcggcgacg tccgccagca ggtgggcggc gcgccccacc tcgttgcggc gcgcgtagtt
     1861 gaacagcagg tcgagaccgg cgctcacctc ggccaggaca aagtcggcgg aacgggccag
     1921 ggcgcgcgct tcttccgcca gcgcctcgtg gccggcgtag tcgaacacct cgaggtgcaa
     1981 gccggccaga ttcgccaccg cacgcgccag caggggccag acctcatgct cgcgactgag
     2041 gtgctgcgcc tcgccaaagg cacgcacggc ctccgtgtac tgcccggtgg cgaccagggc
     2101 gagtcccagg tcctgcagcg ggaagatctg cttgagcggc tggccttgcc gcactgcgac
     2161 ggctgcacgc agagaggtca gcgcctctgg gtaccgaccg agccagtagt acgtcctgcc
     2221 aaggtgatgg tgtgcatccc cccctcccgc taacgcgcgc tcatagcggc tcaccgcctc
     2281 cgtaaggtgg ccgacgttgg tttccacatc gcccagtttc accatcgcgg ccaccaccag
     2341 atcagcgcgc tgcgcttcct cggcttgccg caaggcctcg ctcacgtggt gccgctgcgc
     2401 cgcgacgtcg tgcagataaa agtcgacctc cgccgaggcc agcagcagct ggacctggcg
     2461 gtcgagctgt tccagtcctg ccgccgccag ggccgccgcg aaggcgggac gcgcttccac
     2521 ccacaggcac gcttccaaca gtgcctggcc gcgcttgtgg tgcaacgagc ttgccaggtc
     2581 cgacaagccg gcctcttccg cgatggcaac cgcctggccc aaaagacccg cggcctggcg
     2641 ctgagcagcc gcgcggccgg cgcgttgggc cgcctggctg agatagtcaa tcgcccgctc
     2701 ggcgtggcca gcctccaccc cctggtacag caggtgggct gcggcatagc ggcgcagcaa
     2761 ttcatgcaac gcataccatc caggaccgct cacctgtagg agcgatttat cggccaggct
     2821 ggccagcact ggcagggagg ccccaccgat ctcgcgagcc gcctccaggt caaacggacc
     2881 ccgaaacacg gacagtctgg cgagtgccgc ttgctcgggg ggggaaagca cattccaggc
     2941 ctggtcaaac accgcccgca ggctgcgatg ccgctctggc acatcccgca gcggggaggc
     3001 aaggaagtcg aggctggccg ccaactgctc ggcgatgtgc gagcagggga gcagccgcac
     3061 ccaggcggcg gccagttcaa gcgcgagcgg catcccttcg acctgctggc agatgcgcag
     3121 cacatcggga gccatgccct ggagcgcaaa gtccggctgc acgcgctgcg cactctgcac
     3181 aaacaactgg accgccgagt agctcgccag gtggtcatcg tcggcgtcgg cggtcagggg
     3241 atagtccagg ccatcgaggg gcaggaccca ttcgccgtgc aggttgagcc gttcgcggga
     3301 ggtgatgagc agcttcaggt ggggtgcgcc cgccagcagg tcgaccagca ccggcacccc
     3361 gtccaacacg tgttcaacgt tatccagcag gagcagcacg gttttgtcac gcagggaggc
     3421 ggccagttgc acggcgggcg cttcgtgtcc taccagcgag tactccagga cgtcggcaat
     3481 cgcaaagagc cacgcgtcag ccgacgtgac cgccgccagc gagacgaaat gcgcgccgtg
     3541 ggcaaagctg gtgcggttcc tgtgggccgc ctccacggcc aggcgcgtct tgccgctgcc
     3601 gcctggccca acgatcgtca tcaagcggga gtcgggggcg tcgagcaggc gctgaaggta
     3661 ggcgagctcg cggctcctgc cgacaaaagc cgtgggctgc gacggcacat ggtgaaccgg
     3721 tcctgcccca ggagatgcca tcgtgggagg taccgcactc agcccccgcg caaacgccac
     3781 aaaggccgcc tgcagcggcg gctggatcgc caggacagcg gccaggcgtt cggcgatctg
     3841 cttggaggga cgacggaccc cggcttcgat cttcttcatc gtcaccgggg cgcagcccac
     3901 gcgctgtgcc aactctgctt gcgtcaggtc aagggccttg cgttgctgtc tcagccaggc
     3961 gccaaacggg tgatctggtt gcatcgtgct tccttgcttg cttgctccgc tcatgcgcat
     4021 gcgttacccc aagcccgccc tgttgaacag tctatcacat ctgcatccgc ttcgctaccg
     4081 gcctggcgtc atagggctcc tctcgcgatg ggagcgactt tccgccccgc gcaacggatc
     4141 gcgcgtgtat ccctgcttgt acccctggcg agaccggcac tggtatcccc gaacggcttc
     4201 cagtccgagg cctcgtatgc tacagttgct tcagacacac atctttgcgc atcacagtat
     4261 tcatgcaaga aggagatcac ccatgaccaa gaccatttta cagatcaact accggttcaa
     4321 cgcatcccgc gccgatcaca ccgcgttggt gacccccatg gccgcaccga ttgcggcggt
     4381 gccagggctg ctctggaaag tctggctcat gaacgaaaac gagcaggagg caggcgggat
     4441 gtacctcttc gagagcggtg aggcagctca ggcctttgtc agcagcccgc ccatcacgag
     4501 ctttgcggcg catccctcgc tctcctcttt cagcgtcaag ctgtttgagg ccgttgaatc
     4561 gctcagcaaa ataacacgcg gaccgttgct ggtggccgag accgtctgat gagcgcacct
     4621 ggccatcggt gagagcgtgc cgagcgtgcc acggccctgc tgctcatggt cgccaagtgg
     4681 ccgtgaaggc gaagattcct gatcagcaaa taaagtgtcg gtccgcgcgt cactcttgca
     4741 gtacctatcg actgctctcc tgaaagagtg catcgtcagt gaagattctt tacaggtccc
     4801 tgagtgtaaa tttgggaatt ccatgggtct ggcacaattt aggtgaagga tgggcttctc
     4861 tgacgagtgt tctccttttt ctgagcgtgg aaagaagggt caaatagctg ctactgatgt
     4921 gctttcacca agattgagcc agttccactg aatggcggcg aaatttgaga attctttgac
     4981 gtaatagcgg cgttgccttt tccctcccaa agctctggaa aaggctttgc aggctggttg
     5041 aagagctttc tttttgagaa ggccatctgc gagaagcgtc atatcattat caaaaaacta
     5101 cgccattcag taatcacagt tacacctaaa ggagcacaca gttgtgaaaa aaagaccttc
     5161 acccgtttgt gcggagagag gcaggctcat gcccaggcaa cgatttgaac gaaatactgc
     5221 gacccatggg cgccatcagc ttcgcccatt gctcactgat cccacccaac tgatctatga
     5281 gatcattccg ttccaacttg ctctagggag gactcttgag gatggcgtgc tcacccatgg
     5341 gtctgctgtg cagatgcatc tagtactcca gaagagagga gagttgtatg cccttatttg
     5401 gacgatctgg ttcccccaat gtgaagcaac tccgccaaag ccgggatgtg gaaggattgc
     5461 tggcagctct cactgatccc accgaccagg tacggcaagc ggccgcagac gccttaggcc
     5521 acatcggaga tgctcgtgcc gtagaggttt tgatcaccgt actcacggat ccaggcagcc
     5581 cagaacaagc agcaaacaga gcggccttta tgttaataca tgtgagcgga gatcttcatg
     5641 ccgtcaagcg cctgcatttt agtccacaga atgaagcgta tcgggatgct gtccgattgc
     5701 aaagctgcgc aaacttttga tcgggtatac ttgttgagta aaagtgaagt tgagcagtag
     5761 aaggcattcc tcttgctgcg tgcagagcag cctggcatgc cgaggaggga caaatcatga
     5821 ccgtactgac caccggggac acggcggtcc atcttctcca ctttatggcg cccaggactg
     5881 gccaatcgtc cggcactggc tctatcgttg tggatgctgt catcagtgtc ctgttcttcc
     5941 tgattggcct ctttagcctc tatgtcagtg tgagaggcgg gctgaatgtg ttcaatctga
     6001 gtcgaaacag cgtcgtggtc aaaggctcca tcactgccca ccgtagccgc tccacgggcg
     6061 ctgggggccg agtggtgaga gctgtcggtg agcagtacct cgctacgtat cgatacgagt
     6121 atggagggaa gacctactcc catgagcagg cagtgggcgg tattcccctg gtgggtgaag
     6181 cggtctcagt ccgctgcgtt gcgcaccatc cgaacgtagc gctactggaa ggcccagcca
     6241 gtgcatctct gatcaaagag cgcaaagttg ccctggtcgt catgcccttg ctcgcggcga
     6301 cctttcttgg cgtggcgatc tttgaggcgc tgcaactgct cagatgagtc tgcttcgatg
     6361 ccctccaccc attcgagggc ctgagcgagg atggatctcg tcgggcatca gggacagcga
     6421 ggtgctcagc atttgacctg ttctacttga tgggcgtttc gactacttta gacgtttggg
     6481 caaccccgtt tacagaggat tgcccaccgg aggaatgttc cggctcgatc aaccatgaag
     6541 aagttggctg aaaaagccaa gggagaagga atcctccacc aaacgtttac aacggatgct
     6601 cctggcaaaa gtgagggtac catgcaagag aagaaggaac tgttagaacc ctccacgcgg
     6661 aagaagccgg gtggaacagg cgcaggcgat gccgcgctta ttgctcatct ccagcttggg
     6721 ccagtcgaag aggtctacac acttgcccgt gtccagcgaa tt
//
LOCUS       DASZAK010001206         6315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_529069, whole genome shotgun sequence.
ACCESSION   DASZAK010001206 DASZAK010000000
VERSION     DASZAK010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529069"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..366
                     /locus_tag="VGT44_21255"
     CDS             25..366
                     /locus_tag="VGT44_21255"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2583401.1"
                     /translation="MLEPLSLHPLGTTNLLVPSLCVGCAALGDMPGTFAYSVAEAQAL
                     ATIRDIFDDPISFADTAASYGDGESERLIGMVLRERGGLPPGFVLYYCWYQRAGALTG
                     NCEVGPTSPAG"
     gene            444..1421
                     /locus_tag="VGT44_21260"
     CDS             444..1421
                     /locus_tag="VGT44_21260"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV2583402.1"
                     /translation="MVSLSDASVPGPAEALIRIRQVGICGTDYHAFHGNQPFFTYPRI
                     LGHELGVEIVAVGTDVRHLVKGDHCAVRPYLSCGSCPACLRGKPNCCVYLQVFGVHVD
                     GGLREFTVLPAAYLHPARTLTFEQLALIEPLAIGAHAVAVAEIAKEERVLVVGVGPIG
                     LAVTQFALLMGAQVIVTDISQQRLAFCQNLWPSVTCIDGREPVVETLEHLLGDDLPTA
                     IFDATGNAQSMQATFTCVGYGGRVIFVGLSQKDITFSDPLFHSREVTLFASRNALAAD
                     FDRIIAALQADEFDLTPWITHRATLETAADAFPQWSDANSVMIKGLISI"
     gene            1561..3075
                     /locus_tag="VGT44_21265"
     CDS             1561..3075
                     /locus_tag="VGT44_21265"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HEV2583403.1"
                     /translation="MEMQLSDSTRQIFTQVRVYPSSVTQQGELTYALGEPKQGQRHLV
                     ILAPPACRLLEQFVGEASAGGDQTMLVGPLESHNARTLRSQLSWLNPVTLGLGTSAGF
                     GDRLGIATPGHLRALREAGGHIAPIAAQQSIREMTRTGRSPQQVFDDAMWGVFAEGWR
                     AGFAADADHLKSTEDIDRCVAAGYTFYTFDPGEHVDHTADTAPAAALHQTFEAIPWSA
                     LEDSPKDLLARYLAPQHVEGRILQFDGETLVRASVKYGRAIAHVARLYRHLATVSSGR
                     AWEVEISVDETETPTTHAEHFYFATELRRLGVQWVSLAPRYVGRFEKGVDYIGDLASF
                     TADFAIHAAIARTLGPYKLSLHSGSDKFSIYAIAARETRGMVHLKTAGTSYLEALRTV
                     AQVQPDLLRDIYAFSLERYETDRASYHVSAELHRAPTPTDMPDAAVPMLLDQFDARQI
                     LHVTFGSVLTARDPGGRRRFYDRMMEVLNAHPEAYAANLRSHFLRHLRPFAAYS"
     gene            complement(3408..3917)
                     /locus_tag="VGT44_21270"
     CDS             complement(3408..3917)
                     /locus_tag="VGT44_21270"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEV2583404.1"
                     /translation="MANILETARAWHAYNKYVKGLESVEGLIEALSGPGPFTVFIPTD
                     AAFERRSDDQQANLFADPGKLAKVVKYHIVQGYFTADDLLDRVFLKTLEGPRLSVWSD
                     ISTTPLGEEETDIDQDALSYIVRESVTTQVRESIKINGANIVEANIKADNGILHVIDK
                     VLVPPFTRL"
     gene            complement(4174..5022)
                     /gene="phaZ"
                     /locus_tag="VGT44_21275"
     CDS             complement(4174..5022)
                     /gene="phaZ"
                     /locus_tag="VGT44_21275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006112 - energy reserve metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly(3-hydroxyalkanoate) depolymerase"
                     /protein_id="HEV2583405.1"
                     /translation="MASETGRPQAETRGIFVKGQLLRVVIRKGDGASTPLLMMNGIGV
                     NLEVLQPFVDALNPAIEVIRFDVPGTGGSPAPRIPYTLSMHARLAAQILDQLGYGQVD
                     VLGISWGGALAQQFAFQYPARCRRLVLVSTGTGAFMIPGRPSALVKLATPRRYRDPAY
                     LERIAGEIYGGKMRTRPDLAREFARMASVGSALGYFYQMLGATGWTSLPWLHRLKQPT
                     LILHGDDDPLVPLVNAKIMHRLIYHSKLYIFHDGHLGLGTSARELAQVVDKFLAAPAA
                     SSSDQA"
     gene            complement(5077..>6315)
                     /locus_tag="VGT44_21280"
     CDS             complement(5077..>6315)
                     /locus_tag="VGT44_21280"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2583406.1"
                     /translation="YETGGASVVRGLTHMLEDLANNGGMPSQVNMKAFQAGKDLGISP
                     GAVVFKNEVLELTQYKPATPQVHQRPLLIIPPQVNKFYVMDLAPGKSIVEYLVANGQQ
                     VFAISWRNPTPEQRDWGFEAYDRAMLEAIEAIRSITGSPDVNMVGSCLGGMTLATLLG
                     HLAALGDRRVHAVTFLVTVLDTSVESTMGLFATPEMIATARESSRMRGVVEGQEMARV
                     FAWLRPNDLVWNYWVNNYLIGKDPAAFDVLYWSNDTTRLPARLHSDLLDLFETNAFTH
                     AGTLNVLGTPIDLSKVTSDAYIVAGTTDHITPWQGCYATTQLLGGNVRFILSNSGHIQ
                     ALLNPPGNPKASYLVSERHPADPDQWLAKAQKRAGSWWDDWRDWLGQRSGEQKAAPRE
                     LGNKQYPAGKQAPGSYVFEP"
BASE COUNT         1249 a         1842 c         1867 g         1357 t
ORIGIN      
        1 gcgcccagaa ggagcgtgta accgatgctg gaaccgctat ctcttcatcc cctggggaca
       61 accaatttac tggtgccgtc cctgtgtgtg ggctgcgccg cacttggaga catgccagga
      121 acatttgcct attcggtcgc agaagcgcag gcgctcgcaa ccatccggga catcttcgac
      181 gacccgattt catttgcaga cacggcagcc tcttatggtg atggagagag cgaaagactc
      241 attggtatgg tactgcggga gcgcggcggc cttccaccag gatttgtcct ctactattgt
      301 tggtatcagc gggccggagc gcttacagga aactgtgagg ttggtccaac aagtcctgcc
      361 ggatgatatc tggcaggaaa tcgcagaaga agaaaggatc tcagatgcgt gcgcttcgtc
      421 ttgatgctcc tggtaaaata cacatggttt ctctttctga tgcatctgtg cctggacctg
      481 cagaggcact gattcgcatc aggcaggtag gcatctgcgg gactgactac cacgcctttc
      541 acggcaatca gcctttcttt acgtatccaa gaattcttgg tcatgaactc ggtgttgaaa
      601 tagtggcggt cggcacagac gtgcgccatc ttgtgaaagg cgatcattgc gcagtacgtc
      661 cctatctctc ttgcggttcc tgcccagcct gtctcagagg caaaccgaat tgctgcgtct
      721 atctgcaagt ttttggggtc catgttgacg gggggttgcg cgaatttact gtgctgcccg
      781 ctgcctattt gcatcctgcc aggacactca cctttgaaca acttgccctg atcgagccac
      841 tcgccatcgg cgcccacgcg gtcgcagtag cagagattgc aaaagaggag cgagtgctcg
      901 tcgtaggagt aggcccgatc ggcctggccg tcacacaatt cgcgcttctg atgggtgccc
      961 aggtgatcgt gacagatatc agccagcaac ggcttgcctt ctgccagaac ctatggcctt
     1021 cggtcacgtg catcgatggg cgcgagccag tggtcgagac ccttgagcac ctcctgggcg
     1081 atgatctgcc caccgcaatc ttcgacgcca ctggtaatgc tcaatcgatg caagccacct
     1141 tcacctgcgt tggctatggg ggacgagtga tctttgttgg cttatcacag aaggatatca
     1201 ccttctccga cccgcttttt cacagccgtg aggtgacatt gttcgccagc cgtaatgccc
     1261 tggccgccga ttttgaccgc atcatcgctg cgctacaagc agatgagttc gatctgaccc
     1321 cctggattac tcatcgtgcc acgctcgaaa cggctgcgga tgcgtttcca caatggagcg
     1381 acgctaatag cgtcatgatt aaaggtctga tcagcatcta gtagtgtgtt tggcttcatc
     1441 aaacctcgtg aaactgtcat tctgagcgaa gcgaagaatc ggtgcattgg ggcgaaccga
     1501 cacggcacac tgattcttcg cttcgctcag aatgacagca gaaagagaaa aggagacgac
     1561 atggagatgc agttgagcga cagcactcgc cagattttca cacaggtgcg cgtctacccc
     1621 tcctcagtca ctcaacaggg ggagttgacc tatgcacttg gtgagccgaa gcaggggcaa
     1681 cgccacctcg tcattcttgc gccacctgca tgcaggttgt tagaacagtt cgtaggagag
     1741 gccagcgcgg gcggcgacca gacgatgctg gtcggtccgc tcgaaagcca caatgccagg
     1801 acactgcgca gtcaactttc ctggttgaat ccggtcacac tgggactcgg aacaagtgcc
     1861 ggtttcgggg atcgcctggg gatcgccacc ccggggcatc tgcgtgccct tagagaagca
     1921 ggcgggcata tcgccccgat tgctgcacag caatccattc gcgagatgac ccgcaccggg
     1981 cgaagtccac aacaggtatt cgatgatgcc atgtggggcg tctttgccga gggctggcgt
     2041 gctggctttg ctgctgatgc agatcacctg aagtccactg aagatattga tcgttgcgtg
     2101 gctgctggct acacattcta tacctttgat cctggggagc atgtcgatca cacagcagat
     2161 acggcccctg ctgccgcgct gcatcaaacg ttcgaggcga tcccctggag cgcgctggaa
     2221 gattccccca aggatctttt ggcacgctac ctggctcctc agcacgtcga gggacggatt
     2281 ctccagtttg atggagaaac cctcgtgcgt gccagtgtca agtacgggcg cgctattgcc
     2341 catgttgcac ggctttatcg ccaccttgct accgtttcct ccgggcgtgc ctgggaggtt
     2401 gagatctcgg ttgacgagac cgagacacca acgacccacg ccgaacattt ttatttcgca
     2461 accgagttgc gtcggctggg ggttcagtgg gtgagtctcg cgccacgtta cgtcgggcgc
     2521 ttcgagaagg gggtggacta cattggcgat ctcgcctcat tcacggcaga ctttgccatc
     2581 catgcagcca ttgcgcgcac cctggggccg tataagctga gcctgcactc ggggtcggac
     2641 aaattcagca tctacgcgat cgcggcgcga gaaacccggg gcatggtgca tctgaagacg
     2701 gcaggcacca gctacctcga ggcgttgcgg acggtcgctc aagtgcaacc ggatttactg
     2761 cgcgatattt atgccttctc gctcgagcgc tatgaaacag atcgcgccag ctatcatgtc
     2821 tcggccgagc ttcatcgtgc tcctactccg acggacatgc cagatgcagc agttcccatg
     2881 ctgctcgacc agttcgatgc gcgccaaatc ttgcatgtca ccttcggctc ggtcttgacg
     2941 gcgcgagacc caggcgggcg caggcgtttc tatgaccgga tgatggaggt cttgaatgcc
     3001 catcctgaag cgtatgctgc aaatctcagg tcgcatttcc tccgccacct gcgccctttc
     3061 gcagcctata gctaagaaac gaaggggtag atatcgaaaa tatcttcccc ttcttccttt
     3121 gagcgatatc attaagggac tgcatggagg ctcatgagga acgctgcacg cggaaggtga
     3181 gcgtgaagat atctcctggt ttcacatact gcataaatgg ctcatataac gcctgcatca
     3241 agggaagtgg gtccgcggtc ggctccggca gttcctccat ctctcttgtt cgagatattc
     3301 ccttttcgct tctccctgtt cgagaagtcg cggcgcccgc ccgttcgttc cgagccgcgc
     3361 ggttcaggct cgcgtagttc ggaacgaatg ggggccactg ccaggggtta gagcctggtg
     3421 aatggcggca cgagtacctt atcgatcacg tgcaggatgc cattgtccgc tttgatgttg
     3481 gcctcgacga tgttggcgcc gttgatcttg atgctttccc gcacctgggt ggtcacggac
     3541 tctctaacga tatagctcag cgcgtcctgg tcgatgtccg tttcttcctc ccccagtgga
     3601 gtcgtggaga tgtccgacca cacgctcaac cgtggcccct cgagcgtttt aaggaagacg
     3661 cggtccagca ggtcgtcggc ggtgaagtag ccctgcacaa tgtgatactt gacgaccttc
     3721 gccagcttgc caggatcggc gaagagattg gcctgctggt cgtccgaccg gcgctcaaaa
     3781 gcggcatcgg tgggaataaa gacggtaaag gggccagggc cactgagggc ctctatcagt
     3841 ccttccacgg actcaagccc tttgacgtac ttgttgtacg cgtgccaggc acgcgcggtt
     3901 tccaggatgt ttgccatgaa atgtttctcc ttgttcgcat tctaacgaca ttgatgaggg
     3961 tacaggcgcc ggtttcactc cccggacccg tgtctatgga ccgggagagt tcctgcactg
     4021 atacaccctg tgtactgcag agcgttcgac tacacggttt atcatggaac agagttcgct
     4081 cagttctgtg gcactcgcgt acatagccag cttagacggc gggcgtaaaa aaaagcgtgg
     4141 gaagcgttgt gaaaatctgg ggcagggaag gcatcaggcc tgatctgacg aggaagcggc
     4201 aggggccgcg agaaacttgt cgacgacctg cgcgagttcc cgcgcgctcg tcccaagtcc
     4261 caggtggcca tcgtggaaga tgtacagctt ggagtggtag atcaggcggt gcatgatctt
     4321 cgcgttgacg agtgggacaa gcgggtcgtc atcgccatgc agaatgagcg ttggctgctt
     4381 gagccggtgg agccagggaa ggctggtcca gccggtggct cccaacattt gatagaagta
     4441 gccgagcgcg ctgcctacgc tcgccatgcg cgcgaactcg cgagcgagat cgggccgcgt
     4501 gcgcatcttg cccccgtaaa tctccccggc gatgcgttcc aggtaagccg ggtcgcgata
     4561 gcgccgcggc gtggctaact tcaccagagc cgaagggcga ccgggaatca tgaacgcccc
     4621 cgtccccgtg ctgaccagga ctaatcgccg gcagcgcgcg ggatattgaa aggcgaactg
     4681 ttgggccagc gcgcctcccc atgagatgcc cagcacatcg acctgcccgt agcccaactg
     4741 atcgagaatc tgcgccgcca ggcgcgcatg cattgagagc gtgtaaggga tgcgcggggc
     4801 cggggagcca ccggtgcccg gcacatcgaa gcggatcacc tcaatggccg ggttgagggc
     4861 gtcgacgaag ggctgaagca cttcgaggtt gactccgatc ccgttcatca tcaagagcgg
     4921 cgtgctggcg ccgtcacctt tacgaatgac gactcgcagg agctgcccct tgacaaagat
     4981 gccgcgtgtc tcggcctgtg gtctgccagt ttccgatgcc atcagttatt ctacctcaca
     5041 acgaaacgat acaaaacccg aagggagcgc gacgtgttac ggctcgaaca cgtaggagcc
     5101 gggtgcctgc ttccctgctg gatactgctt attgcccaat tctcgcggcg cggccttttg
     5161 ctcaccggaa cgctgtccca gccagtctcg ccagtcatcc caccaggagc ctgcccgctt
     5221 ctgcgccttc gccaaccact gatcggggtc agccgggtga cgctcgctca cgaggtacga
     5281 cgccttcggg ttgcccggcg ggttcagcag ggcctggata tggccgctgt tgctcagaat
     5341 aaaccgaaca ttgcctccca gcagttgcgt agtcgcatag cagccctgcc acggggtgat
     5401 gtggtcagtg gtgcctgcga caatataggc atcgctggtc actttggaga gatcgatcgg
     5461 tgttcccagc acgttgagcg tgcccgcatg agtaaacgcg ttggtttcga acaggtcgag
     5521 caggtcgctg tgcagccggg cgggcaaacg tgtcgtgtcg ttactccagt agagcacgtc
     5581 gaaggcggcc gggtccttgc cgatcaggta gttgttgacc cagtagttcc agaccaggtc
     5641 gttggggcgc agccaggcga atacacgggc catttcctgg ccctcgacca cccctctcat
     5701 gcgcgaggac tctctggcgg tcgcgatcat ctccggcgtg gcgaacagcc ccatcgtcga
     5761 ctcgacgctg gtatcgagta ccgtgaccag gaaggtcaca gcatgcacgc gccggtcccc
     5821 cagcgcggcc aggtggccca gcagcgtggc gagcgtcatg ccccccaggc acgacccgac
     5881 catgttgaca tccgggctgc cggtgatcga gcgaatcgcc tcaatcgctt ccagcatggc
     5941 tcgatcgtag gcttcgaagc cccaatcacg ctgctcgggc gtgggattgc gccagctaat
     6001 agcgaacacc tgctgtccgt tggcgaccag gtactctacg atgctcttgc ccggtgccag
     6061 gtccatcacg taaaacttgt tcacctgcgg cgggatgatg agcagcgggc gctggtggac
     6121 ctgcggcgtg gctggcttgt actgggtcag ttccagcacc tcattcttga acacaaccgc
     6181 tcccggcgaa atgccaagat ccttgccggc ctggaacgcc ttcatattga cctgagaggg
     6241 cataccacca ttgttcgcaa ggtcttcgag catgtgggtg aggccgcgta ccacgctggc
     6301 cccgcccgtc tcgta
//
LOCUS       DASZAK010001207         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_314344, whole genome shotgun sequence.
ACCESSION   DASZAK010001207 DASZAK010000000
VERSION     DASZAK010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314344"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2505
                     /locus_tag="VGT44_21285"
     CDS             <1..2505
                     /locus_tag="VGT44_21285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2583407.1"
                     /translation="SNDLNYFTKLQSDKTITSYTAVINSTGSLNAAASSFSSFTVEAV
                     DPGNFPVVSQPTFASPANGSVSSLLTNNQVIVTQSFLDNSDKKIGDSFDVYIKSKVGS
                     GETRRVTIAGVVTDTGIFAQSGTLLLVSTHDYQAAALSTPLTYSVIDVTTADQAHTDA
                     AVKDINQHFAFATTQTVADALKSQQSSIDNISKFLEIAGLLALLIGGVGIVNTMQVLL
                     SRRKTEIAMLKTTGYRRVDLYLLFGLEAGLLGLVGGIIGAGAATGVSYIARILVQNTG
                     ITIPFLLNPWTIAGGVAIGLVTALIFGLMPIAQAANIRPLNVVREQSENRGASSFALT
                     IALLVILSILFCALAIVILNNDVLLGIEAVYGAFAFLLLLSVFFGLVVLIVSKLPVPE
                     RFNLKQLAIVMAGVVVSTLVYLALPVFGLIMLGISLTGSLVVLLPRTWKVSTKMALRN
                     IGRQRARTTTTLLALFIGVFTVGLVLALGVDLQSQINSAFSQNLTYNVVATTSGSDTA
                     TLQSKLSSVAGLTKSRSDILTQVLPIAIDGQPLQQALPTGKNRQETFQFLGTVEGYNL
                     AQEAPSLTLSQGRNLAASDVGTNDIVISDLLSSAGPFGMHLKPGDTVTFASVDGKHLQ
                     TATIVGIYSRSSFAGHVGSVLAPAAMVSALSPATTGATTITYMQIDSAQVNNALNTIG
                     KIAPNASVQNLADIGAYIGQMLNNILDILVAIASLSVIAGVIIVANAVALAMLERRRE
                     LGILKSVGYTSSTVLSEVLIENGIVGGLGALLAALLAAAGVTLLGSLFFNLAFSISPL
                     IVLSLVGGSALVAMLTAALVAWGSVRVRPLEVLRYE"
     gene            2556..>4016
                     /locus_tag="VGT44_21290"
     CDS             2556..>4016
                     /locus_tag="VGT44_21290"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEV2583408.1"
                     /translation="MESQRFTQPFRQLRGKLTFSYTLTSVVTFLLVEIIGILVAFSVA
                     SADLNTIVANNLEQEAPLAASYYTNGVPNEVALTTWLHIINVSLSNDWLLSNNLPIYL
                     TVVDPQGQTIASIGTQPVPLHTAIQPYLSSQNWNTLRAQLHSSGLQHGITNQENDGAL
                     VAIAPIDQGGKVVGALVMKIAPPDRLKQFQGFLALVTVTATIVTLIAAISGLIFGYLT
                     ARGLVRRLKRLSGAADRWGRGDFSAAAHDASEDELGQTARQLNRMAEQLQNLLKARQE
                     LATLEERNRLARDLHDSVKQQVFAISMQIGATKLLLNRDPAAAETHLEEAEKLVHQAQ
                     QELTSLIRELRPVALEGKGLVAALRELAADWSQQTNIVANLRVEDSQALSLEVEEALY
                     RVAQEALSNAARHSQATLAQMILTTTEERATLTIVDNGQGFDASRQGRLGVGLLSMQE
                     RMKALGGDAQIESTPGKGTKVVAYCSRTGKTPAKMED"
BASE COUNT          836 a         1287 c         1142 g          751 t
ORIGIN      
        1 tcgaacgacc tgaactactt caccaagctc cagagcgaca aaacgattac gagctacacg
       61 gcggtcatca actcgaccgg ctcgttgaac gcggcggcat cgtcgttttc atccttcacg
      121 gtcgaggcgg ttgatccggg caacttcccg gttgtttccc agccgacctt cgcttcaccg
      181 gcgaacggca gcgtttcaag cctgctcacc aataaccagg tgatcgtcac acagagcttc
      241 ctcgacaact ccgacaagaa aataggcgat agcttcgatg tctatatcaa atcaaaagtc
      301 ggttcgggcg agactcggcg cgtcacgatt gccggagtcg tcaccgatac gggcatcttc
      361 gcccaatcgg gcaccctgct gctcgtctca acgcacgatt accaggcggc agcgctcagc
      421 actcctctga cctatagcgt gatcgatgta acaacagcag accaggcgca taccgacgcg
      481 gcggtcaagg acatcaacca gcacttcgcc ttcgccacga cccagacggt cgccgacgcg
      541 ctgaaatcac agcagtcctc catcgataac atcagcaaat tcctggaaat cgccgggttg
      601 ctggcactgc tgatcggcgg cgtcggcatc gtcaacacca tgcaagtcct gctctcgcgc
      661 cgcaagactg agattgccat gctcaagacg accggctacc ggcgagtaga cctctacctg
      721 ctctttggct tagaggccgg gctgctcgga ttggtaggcg gcatcattgg agcgggcgcg
      781 gccacgggcg tcagctacat cgcgcgcatc ctggtacaga atacaggcat caccattcca
      841 ttcctgctca acccctggac tatcgcgggc ggcgtcgcaa tcggcctggt caccgccctg
      901 atcttcggcc tgatgcccat tgcgcaggcc gccaatatcc ggccattaaa cgttgtgcgc
      961 gaacagagcg agaacagagg cgcgagcagc tttgccctga cgattgctct gctggtaatc
     1021 ctctccatcc tgttctgcgc gctggccatc gtgatcttga ataacgatgt gctgctaggc
     1081 atcgaggcag tctacggcgc gttcgccttc ctgctgctgc tgagcgtctt cttcggattg
     1141 gtcgtgctga tcgtcagcaa actgcccgtg ccggagcgct tcaatctcaa acaactcgcg
     1201 atcgtcatgg cgggcgtggt cgtctcgacc ctggtctacc tggcgctgcc ggtgttcggc
     1261 ctgataatgc tcggcatatc cttaaccggc agcctcgtcg tgctgctgcc gcgcacctgg
     1321 aaggtcagca cgaagatggc gctgcgcaac atcgggcggc agcgcgcccg caccacgacg
     1381 acgctgctgg cgctcttcat cggcgtcttc acagtcgggc tggtgctggc gctgggcgtc
     1441 gatctgcaat cgcaaatcaa cagcgcattc tcgcaaaacc tgacctacaa cgtcgtcgca
     1501 accacatctg gcagcgatac tgctaccttg caatcaaagt tgagcagtgt agcgggcctg
     1561 acaaagtcgc gttcagacat tcttacacag gtactgccaa tcgccatcga cggacagcca
     1621 ttgcaacagg cgctgccgac tggcaagaac cgccaggaaa catttcaatt cctcggcacg
     1681 gtggagggct acaacctggc gcaggaagct ccctcgctga cgctctcgca ggggcgtaac
     1741 ctggccgcga gcgacgtggg tacgaacgac atcgtcatca gcgacctgct gagcagcgcc
     1801 ggtccgtttg ggatgcacct gaagccgggc gacaccgtca ccttcgccag cgtggacggc
     1861 aagcatttgc agaccgcaac cattgtgggc atctattcgc gcagctcatt tgccggccat
     1921 gtcgggagcg tactggctcc ggcggcaatg gtgagcgcgc tcagcccggc cacgacaggc
     1981 gcaacgacca tcacctacat gcaaatcgat agcgcgcagg tcaacaacgc cctgaacacc
     2041 attggcaaga tcgccccgaa cgcctcggtg caaaaccttg ccgatattgg cgcgtacatc
     2101 gggcagatgt tgaacaacat cctggacatc ctggtagcga tagcctcgct ctcggtcatc
     2161 gccggtgtca tcatcgttgc caacgccgtt gccctggcca tgctggaacg tcgccgcgag
     2221 ctgggtatcc tcaagtcggt cggctatacc agcagcacgg tcttgagcga agtgttgatc
     2281 gagaacggca tcgtcggcgg acttggcgcg ctgctggccg ccctgctcgc agccgccggg
     2341 gtgacgctgc tcggctcgct gttcttcaac ctcgcattca gcatctcgcc cctgatcgtg
     2401 ctgagcctgg tgggaggctc cgccctggtc gccatgctga cagcggccct ggtcgcctgg
     2461 ggttcggtgc gcgtccgtcc gctcgaagtg ctgcgctacg agtaaaaagt gctatactaa
     2521 ctttacggta atcgacctga gatcgggaaa aagccatgga gtcgcaacgt ttcacacaac
     2581 cgttccggca actgcgaggg aaactgacat tttcctatac attgacatcg gtagtgacgt
     2641 tcctgctcgt cgagatcatc ggcatcctcg tcgctttcag cgttgccagc gccgatctca
     2701 acaccatcgt cgccaacaac ctggagcagg aagcgcccct ggcggcttcc tactatacga
     2761 acggtgtgcc gaacgaggtg gcattgacga cgtggctgca tatcatcaac gtcagtctct
     2821 ccaacgactg gctattaagc aacaatctgc cgatctatct aactgtcgtc gacccgcagg
     2881 ggcagaccat cgcttcgatc ggcacccaac cggtccctct acatacagcc attcagccct
     2941 atctttcctc gcaaaactgg aatactctca gagcgcagtt gcacagcagc ggcctccagc
     3001 acggcattac caaccaggaa aacgacggcg cgctggtggc aatagcgccc atagatcagg
     3061 gtggaaaagt ggtgggcgcg ctggtcatga agattgcccc gccagacaga ttgaagcagt
     3121 tccagggctt cctggcgtta gtcaccgtta ccgcgaccat cgtgacactt atcgccgcca
     3181 tttcaggctt gattttcggc tacctgacgg cgcgcggcct ggtacggcgc ttgaaaaggc
     3241 tctccggcgc ggcggaccgc tgggggcgcg gcgatttctc cgccgcagcg cacgacgcct
     3301 cggaggatga attaggccag acggcgcgcc agttgaaccg catggccgaa caattgcaga
     3361 acctgctcaa ggcccgacag gagcttgcca cgctggaaga gcgcaatcgc cttgcgcgcg
     3421 atctgcacga cagcgtcaag cagcaggtct tcgccatctc catgcaaatt ggcgcgacaa
     3481 aactgctgct caatcgcgac ccggcggcgg ctgaaacgca cctggaagaa gcggaaaagc
     3541 tcgtccacca ggcgcagcag gaactgacct cgctgatccg cgaactgcgc cccgtcgccc
     3601 tcgaaggcaa agggctggtc gccgcgctgc gcgaactggc tgccgactgg tcgcagcaga
     3661 cgaatatcgt ggcaaacctg cgcgtagaag actctcaggc gctctccctc gaagtcgaag
     3721 aggcgctcta ccgcgtggcg caggaggccc tgtcgaacgc ggcgcgccat agccaggcca
     3781 ccctcgcgca gatgatcctg accaccactg aagagcgggc cacgctcacc atcgtcgata
     3841 acggccaggg cttcgacgca tcgcgccaag gccgcctggg cgtcggcctg ctctcgatgc
     3901 aggaacgcat gaaggctctg ggcggcgacg cgcaaatcga gagcacgccc ggcaaaggca
     3961 caaaggtcgt ggcgtattgc agccggacag gcaaaacccc tgcgaaaatg gaggat
//
LOCUS       DASZAK010001208         3661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_135810, whole genome shotgun sequence.
ACCESSION   DASZAK010001208 DASZAK010000000
VERSION     DASZAK010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135810"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VGT44_21295"
     CDS             <1..1141
                     /locus_tag="VGT44_21295"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEV2583409.1"
                     /translation="PGGSMEAVLSDVVVIDLSRVLAGPYCTMMLGDLGATVIKVEHPG
                     KGDDTRHFGPPYIAGESAYYLGLNRNKQSILLDFSTPEGKERLLKLVSTATVLVENFR
                     PGSLERQGLGYEALHAINPGLIYCSISGYGQTGPYASRPGYDFVAQAESGLMSVTGEI
                     DGDPQRVGSPVGDVSAGMFACMSILAALRVRDHTGKGQYIDISLLETTISLLSNVSSN
                     YLISGEEAQRFGNGHPNIVPYQAFHTEDGYVVVSCGNDRLYQALCHLLAREDLAADPR
                     FATNPQRVRNRKELVPVLQEEFLRRRTDEWLPELRAAGIPCGPINTVSQIFNDPHIQA
                     RGVVWECEHPTAGKIKLSGSPMHLSETPTRLYKAPPLLGEDDDKV"
     gene            1311..2450
                     /gene="dnaN"
                     /locus_tag="VGT44_21300"
     CDS             1311..2450
                     /gene="dnaN"
                     /locus_tag="VGT44_21300"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV2583410.1"
                     /translation="MKITCKQQDLSRGLTAVSHAVSSRSTLPILSNILLSTDHGRLKL
                     SATNLEIGINCWVDAQIHEEGTTTVPAKLLTDFVNSLPQAPVDMALVEDTNTLNVKSL
                     GSNANIKGMDASEFPLIPNAEDGEPPVVLDATLLKEMIAEVAFAAADDDSRPVLTGVL
                     VQVGGEKITFAAADAFRLAVRVAPLPGDDHPRGDILIPARTLTELARILPSEGTVEMI
                     VTPNRSQVLFHTQNLDLVSRLIDGTFPNFRQIIPKEHTTRSVVETKQFAEAIKRAALF
                     ARDSSNITRVKINPGENDGLEPGSLTIEATAEDLGDNTSTINAAVDGPELQIIFNVKY
                     LADVLAVIGTPEVALEANATTKPGVIRPVGADDYTYVIMPMHINK"
     gene            2531..>3661
                     /locus_tag="VGT44_21305"
     CDS             2531..>3661
                     /locus_tag="VGT44_21305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV2583411.1"
                     /translation="MHLSHLSLKDFRNYKQLDLSLGPGLFLFQGENAQGKTNLLEAVA
                     MLATSNSFHATSDREIVNWHASDRITRIAASVKRHNDNVKIEIVLIDPTPQVNGASGD
                     GGSKFIAPNDRQRKHIKINSVPKKGMDLIGLVTVVLFSPNDLSLVDGSPEERRRFLDR
                     GLCQMQPHYCQALLKYRKIVAQRSALLKRIRENQEDPRMLDYLDEKLTELATMITFER
                     RRMVVSLNQHVDELQAIISGGREHLKIVYRPSFHIDPAWDTMETLQNFRTQLNEARRK
                     EIHQGVCLLGPHRDDLEFLVNDVNMLTYGSRGQQRTSALAAKLGELAYMRAVTGEEPI
                     LLLDDVFSELDPLRREHLLSEVLRHEQVLITATDFESFPEEIL"
BASE COUNT          863 a         1001 c          971 g          826 t
ORIGIN      
        1 accaggaggt tctatggaag ctgtactctc tgatgtagta gttattgatc tctcgcgcgt
       61 gctggccggc ccatactgta cgatgatgct gggcgacctg ggagcaactg tgatcaaggt
      121 tgagcaccct gggaaaggtg acgacacacg ccacttcggc ccgccttata ttgctggcga
      181 aagcgcttat tacctggggc ttaaccgcaa caagcagagc attctacttg atttcagcac
      241 gccggagggc aaagagcgac tcctgaaact cgtgagtaca gcgacggtgc tggtcgaaaa
      301 ctttcgtccc gggtcgttgg aacgccaggg attaggctac gaagctctac acgcaatcaa
      361 ccctggactg atctactgct cgatcagtgg ctatggtcag actggcccct acgcatcacg
      421 tcccggctat gactttgttg cgcaggccga atcgggcctg atgtcggtga ccggagagat
      481 cgatggcgat ccccaacgtg tcggttcgcc ggtgggggat gtttcggctg gtatgttcgc
      541 ctgtatgtct atcctggcgg cgctgcgcgt gcgcgaccac acggggaaag ggcagtacat
      601 cgatatttca ctgcttgaga ccactatctc actgctgagc aacgtttcat cgaattacct
      661 gatttctggc gaagaggcgc agcgctttgg caacggtcat cccaatattg tgccgtatca
      721 ggccttccat acagaagatg gatatgttgt cgtctcctgc gggaatgacc gcctctacca
      781 ggcgctctgc cacctgttgg cacgcgaaga cctggcggca gacccccgtt tcgccaccaa
      841 tccacagcga gtgcgcaacc gcaaggagct tgtgcctgtc ttacaagaag agttcctgcg
      901 ccgcaggact gacgaatggc tgccggagct gcgtgcagcc ggcataccgt gcggcccgat
      961 caatactgtc agccagatct tcaacgatcc gcacatccag gcaagggggg tcgtatggga
     1021 atgcgaacat cccacggcag ggaaaatcaa gctatctggt tcgccaatgc atctttcaga
     1081 gacaccaacg cggctgtaca aggcaccgcc tttgttggga gaagatgacg ataaggtgta
     1141 ggggtgcaat caactgcatc tctattcctt ccacttctct gctctatcgt tggaatacgt
     1201 tatagtaaag caacgaattt gtaccacatg ccttgttctc tctattagaa tcctgtatac
     1261 taaccaggaa ctttttcgtg cctttggttg tcccttggag gaaaaacaca gtgaaaatta
     1321 catgcaagca gcaagacctg agccgggggc ttaccgccgt cagccatgcc gtctcaagcc
     1381 gcagcacact cccgatcctg tcaaatattt tactctctac cgatcatggt cgtttgaaac
     1441 tatcggctac caacctggaa attggcatta attgctgggt cgatgcgcag attcatgaag
     1501 aggggacaac aaccgtcccc gcaaaactgc tcaccgattt tgtcaatagc ctgccacagg
     1561 ccccggttga tatggcgtta gttgaagata ctaataccct gaatgtcaag agcctgggca
     1621 gcaatgcgaa catcaagggg atggatgctt ccgagtttcc gctcatcccc aatgccgaag
     1681 atggcgagcc tcccgtggtg ctcgacgcga ccctgctcaa agagatgatt gccgaggttg
     1741 cttttgccgc tgccgacgat gattcgcgcc ctgtcttgac cggcgtgctg gtgcaggtcg
     1801 gtggcgaaaa aataaccttt gccgctgctg atgcattccg cctcgcagtg cgcgttgcgc
     1861 ccttgcccgg cgatgatcat cctcgcggcg acattctcat cccggcacgc accctgaccg
     1921 agctcgcgcg tatcctgccg tctgagggaa ccgttgagat gattgtgacg cccaatcgca
     1981 gccaggtgct gtttcatacg cagaatctcg atctcgtctc acgattgatc gatggcacct
     2041 tccccaattt ccgccagatt attcccaaag agcacacgac gcggtctgtc gttgagacga
     2101 agcaattcgc agaggctatc aagcgtgccg cgctgttcgc ccgcgacagt tcaaatatca
     2161 cacgcgtcaa gatcaatcct ggtgaaaatg atggactgga accaggctcg ctcacgattg
     2221 aggcaacggc tgaagatctg ggagataata ccagtacaat caatgcagcc gttgatgggc
     2281 ctgaactgca aatcatcttc aatgtgaaat atctcgcgga cgtgctggcc gttattggta
     2341 cgcccgaggt ggcactcgag gctaatgcca ctacaaaacc aggcgtgatc agacccgtgg
     2401 gcgcggacga ctatacgtat gtgattatgc ccatgcatat caataaataa cactatggag
     2461 caggggggca aatgcccccc tgccggggaa tgggggcatc cccgaaaatc tcctcctcct
     2521 ggacatatac atgcacctgt ctcacctctc gctgaaagac tttcgtaact acaagcaact
     2581 cgatctctct ctagggccgg gtctatttct ctttcagggt gagaacgccc agggtaaaac
     2641 caatctgctt gaagctgtag caatgctggc gacatccaac tcttttcatg ccacatcaga
     2701 ccgtgaaatc gtcaactggc acgcgtctga tcgcatcaca cgcattgctg cttcggtcaa
     2761 acggcacaac gataacgtga agattgagat tgtgctcatc gacccaacac cgcaagtaaa
     2821 tggggctagc ggcgatggag ggagcaaatt catcgcgccc aacgacaggc aacgcaagca
     2881 cattaagatc aatagcgtgc caaaaaaggg gatggatctg atcgggctgg tgacagtggt
     2941 gctgttctca cccaatgatt tgagcctggt agatggttcc ccagaagagc gacggcgctt
     3001 tctcgatcgc gggctttgcc agatgcagcc tcactattgc caggcgttgc tcaagtatcg
     3061 taagatcgtc gcgcagcgat ccgccttgct caagcgtatt cgcgaaaacc aggaagatcc
     3121 acgcatgctg gactacctgg atgaaaaatt gaccgaactg gcaaccatga ttacctttga
     3181 gcgccggcgt atggtcgtct cgctcaacca gcacgttgat gaattgcagg ccattatcag
     3241 tggcgggcgc gaacatctca agattgtcta tcgcccatca tttcatattg atcctgcctg
     3301 ggataccatg gaaaccctgc aaaacttccg cacacaattg aatgaagcgc ggcgtaaaga
     3361 aattcaccag ggggtgtgct tgctggggcc acatcgagat gacctggagt ttctggtgaa
     3421 cgacgtaaat atgctcacct atggatcgcg cggtcaacag cgtacatcgg ccctcgccgc
     3481 caaacttggg gagctagctt atatgcgtgc agtcaccgga gaagagccga tcctgttgct
     3541 tgacgatgta ttcagcgagc ttgatcccct gcggcgcgag catttgctaa gcgaagtatt
     3601 gcgacatgaa caggtgctga ttaccgccac agatttcgaa agttttcctg aggagattct
     3661 a
//
LOCUS       DASZAK010001209         1615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_492429, whole genome shotgun sequence.
ACCESSION   DASZAK010001209 DASZAK010000000
VERSION     DASZAK010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492429"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VGT44_21310"
     CDS             complement(<1..395)
                     /locus_tag="VGT44_21310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583412.1"
                     /translation="MKRSDSGDFYMLYIEPEDDRHSVYDALTAISRQHKKPVVMMLPV
                     TGRARAFARPEDFSDLKRIKRQYELLILFVSASNEHLRHLAARNSFPAYPSIDELSDA
                     LLRGQISFAHQRVMAKTPTPSIPTSRRIE"
     gene            complement(418..1527)
                     /locus_tag="VGT44_21315"
     CDS             complement(418..1527)
                     /locus_tag="VGT44_21315"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV2583413.1"
                     /translation="MRKMKFFAGAAFFLNTLCFATYYAVAKEALGRIDPIMFTYFEMM
                     TLAPVSVIILLCSWRHITRAVVRRGLLLGSTLCLALFTIAIALNYTSATSVAFFPALN
                     GFLAGVFAWLVLRQPMRKTTWLAGLLSLAGVVLLAFNSSMGGWRGPLIAFLGGLFFTG
                     YVFVSDMGAKQSGQEGKQGDHTHWPLFGIELLTMAVWANLVVLLFGDWHTIHPSLPKD
                     IYVIAYVAGVCTFLPTLIAVLMQRYISPVTVSFIYILEPVLGALISTLYLGESLPLAG
                     YAGGLLIVLGAIIHTWASARVTQTREIEEIDETRVIVEPGIEYYEEYDLLPDWESARS
                     PLQRGLVTYSVARKGEKQLLQQRRQQRISRLRLVG"
BASE COUNT          359 a          482 c          465 g          309 t
ORIGIN      
        1 tcctctatcc gccgcgaagt agggatggat ggagtggggg tcttcgccat gacgcgctgg
       61 tgcgcgaatg agatttgccc ccgcagcagc gcgtcgctca gttcatcgat ggaagggtat
      121 gccgggaaac tgttgcgcgc ggccaggtgc cgtagatgct cgttactcgc gctcacaaac
      181 aggatcagca gctcgtattg ccgtttgatg cgtttgaggt cgctgaaatc ttctgggcgc
      241 gcgaatgccc tggccctgcc cgtcaccggc aacatcatca cgactggttt tttgtgctgg
      301 cggctgatgg cggtcagcgc atcatacacg ctgtggcggt cgtcctccgg ctcgatgtag
      361 agcatataaa aatcaccgga atcgctgcgc ttcatgtatt cctccgatta cggcatttca
      421 accaaccagg cgcaaccgcg atatacgctg ttggcggcgc tgctgcaata attgcttttc
      481 ccctttgcgc gctacggaat atgtcaccaa tcctcgttgg agtggtgatc gtgccgactc
      541 ccaatcgggc aacaaatcgt actcctcgta gtattcgatg cccggctcaa cgatgaccct
      601 ggtttcgtcg atctcctcga tttccctggt ctgtgtaacc cgcgcgctcg cccaggtatg
      661 gatgattgcg ccgagaacga tgagtagccc gccagcgtaa cccgccagtg gcagcgactc
      721 gcccaggtag agcgtggaga tgagcgcgcc cagcaccggc tcgaggatat agatgaatga
      781 gacggtaacg ggtgaaatgt agcgctgcat cagcacggcg atcagcgtgg gcaggaaagt
      841 acacacgccc gcgacatagg cgatcacgta gatatccttc ggcagcgagg gatggatggt
      901 gtgccagtcg ccgaagagta gcacaaccag gttggcccac accgccatcg tcagcagttc
      961 gatgccgaag agcggccaat gtgtgtgatc cccctgcttg ccctcctgcc cgctctgctt
     1021 cgcgcccata tcgctaacga agacatagcc cgtgaagaac agcccgccca aaaaagcgat
     1081 caacgggccg cgccagccac ccatgctgga attgaacgcc agcagcacta cgcccgccag
     1141 ggagagcagg cccgccagcc atgtcgtctt gcgcatgggc tgccgcagca caagccaggc
     1201 gaagacaccg gccaggaacc cgttcaaggc cggaaaaaac gctacgctcg tcgcagaagt
     1261 atagttcagc gcgatggcga tggtgaagag cgccaggcaa agtgtgctgc cgagcagcaa
     1321 gccgcgccgc acgaccgcgc gcgtgatatg ccgccacgag cagagtagga ttataaccga
     1381 gacgggcgcg agcgtcatca tctcgaagta cgtaaacatg atcgggtcga tgcgccccaa
     1441 cgcctctttc gccaccgcgt aatacgtggc aaaacacagt gtattcaaaa agaaagctgc
     1501 tcccgcgaag aatttcatct tcctcatagg cgttcccccc gatcaagatg caacagatac
     1561 cggctccgat gctttagagc cttttaaagt tccaggcatg gcgaccgcaa gggat
//
LOCUS       DASZAK010001210         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_599794, whole genome shotgun sequence.
ACCESSION   DASZAK010001210 DASZAK010000000
VERSION     DASZAK010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599794"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VGT44_21320"
     CDS             <1..846
                     /locus_tag="VGT44_21320"
                     /inference="COORDINATES: protein motif:HMM:NF019643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BBE domain-containing protein"
                     /protein_id="HEV2583414.1"
                     /translation="RKDIHEQFALTPGAQVVPTEQELLAQWGFIAQEITFLLWPLEQA
                     AEVMKFWRDFILNAPEEINGWFAFLTVPPAPPFPEQFHLQKMCAIVWCSSAPPEQAEK
                     LFQPIRASIPPVVDFAGPIPWPVLQSLFDALYPPGLQWYWKNDFFSDLSEKAIELHVK
                     YGAQLPTMHSTMHIYPINGAAHRVGKNDTAFSFHDANFDEVIVGVDPDPTNNERMIEW
                     ARDYWMALHPYSAGGGYVNMIMDEGQDLVQAAYCDNYTRLAQIKATYDPDNLFHVNQN
                     IKPTR"
     gene            997..1344
                     /locus_tag="VGT44_21325"
     CDS             997..1344
                     /locus_tag="VGT44_21325"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV2583415.1"
                     /translation="MKYTMQLISRDELKRKLEREDQFKLVMALGDWEYRAKHIPGSLH
                     FLTPQEALASLAQDDEIVVYCSNSACPASAFAYEYLVHHGYKHVRRYAGGILDWEEAG
                     YPLEGEMVAAKTP"
     gene            1368..3026
                     /locus_tag="VGT44_21330"
     CDS             1368..3026
                     /locus_tag="VGT44_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2583416.1"
                     /translation="MKPVIMAVDDEPTALAAIERDVQREDGADFRIVSATSGNEALAM
                     LKQLKLRNEAVALLLIDQRMPHMTGVEFLEQAMELYPDAKRVLLTASSDGDVPISTIR
                     PAKPDYYLRKPWDSPEEQLYPVLKDLLADWQVTYRPPSEGLRVIGHRWAANTYRVRDL
                     LARNQVRYQWFDLDTSEEACRLLAALGSETPSLPVLLFPDGSWLADPTPIEVAEHFGL
                     RTQAQLPFYDLIIVGAGPAGLGAAVYGASEGLHTLLIEREAPGGQAGMSSKIENYLGF
                     PAGLSGAELTRRALMQASRFGVELLLPHEVTGISVEGSTRLVHLADGATLRCHTILLA
                     TGVSYRTLDVPGSERLQGAGIYYGAGMSEAPLYRDQDIYLVGGANAAGQAAMYFSRYA
                     RSVTLLVRGPSLDESMSQYLIDQITAIPRIKVLTSTRVVEVKGETNLEAITTTNTETC
                     ETQVLPTTALFVFIGAMPQTDWLAGLVERDEHGYILSGLDMLHDGYRPAGWTLERDPF
                     PLETSVPGVFVAGDTRLHSMKRVASAVGEGAMSVRLVHDYLRSL"
BASE COUNT          650 a          901 c          952 g          625 t
ORIGIN      
        1 cgcaaggata tccatgaaca gtttgcattg acgccaggtg cgcaagtggt gccaacggaa
       61 caggagttgc tggcccagtg gggttttatc gcccaggaaa ttactttcct gctctggccg
      121 ctcgaacagg cggcagaggt gatgaagttc tggcgcgact tcatcttgaa cgctcccgaa
      181 gagatcaacg gctggttcgc gtttctcacg gtaccaccag cgccaccgtt cccggagcag
      241 tttcacctgc agaagatgtg tgccatcgtg tggtgctcta gcgcgccgcc ggagcaggcc
      301 gaaaagctgt tccaaccaat tcgagccagc atccctccag tagtggattt cgccgggccg
      361 ataccgtggc cggtgctgca gagcctgttc gacgcacttt accctccggg tttgcagtgg
      421 tattggaaga acgacttctt cagcgacctg agtgagaagg caatcgagtt gcatgtgaag
      481 tatggtgcgc agctaccgac gatgcactcg acgatgcaca tctacccgat caacggtgcg
      541 gcccatcgag tcggcaagaa cgataccgcg tttagcttcc atgacgctaa cttcgatgag
      601 gtgatcgttg gggtcgaccc cgacccgacc aataacgagc gcatgatcga gtgggccagg
      661 gactattgga tggcgctgca tccctattca gcgggtggag gttatgtcaa tatgataatg
      721 gatgagggcc aagaccttgt gcaggcagca tattgcgaca actacacccg gctggcacag
      781 atcaaagcaa cctatgatcc ggacaatctg ttccacgtga atcagaatat caagccaacc
      841 aggtgagacc acccgagagc ttgatggata ggagcacttc gcggaccgcg ctcttgcgat
      901 tcgctccaat cgtagttgac gaccggctag tgagtaccag atgccaccat cagcgacgta
      961 ctcatgctcg aacccgaatg aaaaggagat tgaacgatga aatataccat gcaactgatc
     1021 agccgtgatg agttgaagcg gaaattggag cgcgaggatc agtttaagct agtgatggct
     1081 ctgggggact gggagtatcg tgcgaaacat atccctggct cgcttcactt cctcacgcca
     1141 caggaagcgc tggcctcgct cgcacaggac gatgagattg tcgtctactg ttccaactct
     1201 gcatgtcctg ccagcgcctt cgcctacgag tacctggtgc atcacggcta caagcatgtg
     1261 cgccgctatg cgggcggcat tctcgactgg gaggaggccg gctatcccct cgagggggag
     1321 atggtcgctg ccaagacccc gtaagcaaga ggaaggaggg tatgacaatg aaacctgtaa
     1381 tcatggcagt ggacgacgag cccaccgcgt tagcagccat cgagcgtgac gtacagcgcg
     1441 aggatggtgc tgactttcgg attgtgagtg cgacctctgg caacgaggcg cttgcaatgc
     1501 tcaaacagct caagctgcgc aatgaagcgg tggccctcct gctcatcgat cagcgcatgc
     1561 cacacatgac gggcgtcgag tttctggagc aggccatgga actctacccg gatgccaagc
     1621 gcgtcctgct gacggcctct tctgatggag acgttccgat ctccaccatt cgcccggcga
     1681 aacccgacta ttacctgcgc aagccatggg actcacccga agagcagctc taccccgtcc
     1741 tgaaagacct gctcgccgat tggcaggtca cctaccgccc accttccgaa ggactacgcg
     1801 tcattggcca tcgctgggca gccaacacct atcgtgtgcg ggatttactc gcccgcaacc
     1861 aggtgcgcta ccagtggttc gatctcgaca cgtccgagga ggcgtgccgc ctgcttgcgg
     1921 cccttgggtc ggagacgccc tccctgccgg tgctgctctt ccccgatggg tcctggctgg
     1981 cagatcccac acccatcgag gttgctgaac actttgggct gcggacgcag gcccagttgc
     2041 cgttctacga cctgatcatt gtgggagcgg gaccggctgg gctgggggca gctgtctatg
     2101 gcgcctcgga aggcttgcat accctgctca tcgagcggga agccccaggc ggccaggctg
     2161 ggatgagttc gaagattgaa aactacctgg gcttcccggc cgggctgagc ggggcagagt
     2221 tgacacggcg tgccctcatg caggcctccc gcttcggggt cgaactgctc ttgcctcacg
     2281 aggtgacagg catcagcgtc gaggggtcta cccgcctggt tcatttggcg gatggggcca
     2341 cccttcgttg ccatacaatc ctcctggcca ccggcgtttc ctatcgcacc cttgatgtgc
     2401 ccggcagcga gcgattacag ggggcaggca tctactacgg tgccgggatg agcgaagcgc
     2461 cgctctaccg tgaccaggac atctacctgg tgggtggggc caatgccgcc gggcaggcag
     2521 ccatgtactt ttcccgctac gcccgcagcg tcacgctgct ggtacgtggg ccatcgctgg
     2581 acgagagcat gtcgcagtac ctgatcgatc agatcacagc gataccgagg atcaaagtcc
     2641 tcacgtctac gcgcgtggtc gaggtcaagg gagagaccaa tcttgaggcc atcacgacca
     2701 ccaatacaga gacttgtgaa acgcaggtgc ttcccacgac agcgctcttt gtgtttattg
     2761 gcgcaatgcc acagactgac tggttagccg gtctggtgga gcgggatgaa cacgggtata
     2821 tcctgtctgg actcgatatg ctccacgatg ggtatcgccc ggcaggatgg acgcttgagc
     2881 gtgacccgtt cccgctcgag acaagtgtgc ccggggtgtt cgtggccggg gatacacggc
     2941 tgcactctat gaagcgggtg gcttcggcag tcggggaggg agcgatgagc gtgcggctgg
     3001 tgcatgatta cctgagaagc ttgtgatgct ggtgcaggcc ctttcagacc cagcaccaga
     3061 tctcccgctt cgcggatagc acggacaagg ggctgcaagg cggactggcg cagatgaagg
     3121 aagcttcc
//
LOCUS       DASZAK010001211         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_30993, whole genome shotgun sequence.
ACCESSION   DASZAK010001211 DASZAK010000000
VERSION     DASZAK010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30993"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..466
                     /locus_tag="VGT44_21335"
     CDS             74..466
                     /locus_tag="VGT44_21335"
                     /inference="COORDINATES: protein motif:HMM:NF024485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEV2583417.1"
                     /translation="MTLLDITLQIFRYKPGQQPRYDTFTVQVEDTAHVIDAIDAVWAK
                     HDHTLTFRRACHHSSCGSCALRINGVEKLPCITRFSEVWDGSSPLRLEPLRNFPIVSD
                     LVVDVSGFFWAEWQTWVDRRCSLKGKGD"
     gene            613..2081
                     /locus_tag="VGT44_21340"
     CDS             join(613..1492,1492..2081)
                     /locus_tag="VGT44_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611833.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HEV2583418.1"
                     /translation="MLSGLWRMTLVVRQCIQPSCPRFHQPYRPEEEGRWALPHGEFGL
                     EVIALIGQWRFREHRSVPEMHQALQARGMSIAQRSVTYLMQRYEELVTLRITDQERIK
                     ARLQKQGHVILALDGLQPDVGHEVLWVVRDCLSQEILLARPLLSSTQGDLTALLKEVK
                     AQLKQLKVAVKGVISDGEETIGSAVAFVFPGVAHQLCQFHYLKDATKLLYEADRHAKT
                     QLKKQLRGVRPIERALEEHSTPENEAIRGYCLAVRASLTDDGRSPLQPSGLKLYDRIT
                     QVNDSIARVQEKKGCHRPLKLLQQLLTKGVKATAALWSPLQRAYGLVHQAAQILANEE
                     QHTGAQVRERYLAHVCQMQGQKASLEPLGEAIEHFCHITDNFASGLFECYDVEGLPRT
                     NNDLEHCFGVARVHERRATGRRGAIPGVVVRGSVRVIAAVVTKQQSFSAEELQPRDYQ
                     RWRDLRAHLQQCEETRRQQFRFRKNPATYLAALEAQLLR"
BASE COUNT          571 a          662 c          702 g          556 t
ORIGIN      
        1 gcattgcggc cgagtgtcgc acgcgcgagg taccactcgc ctcgtatcgt ttgttctttc
       61 aaggaggtca acaatgacgt tgctcgatat tacgctgcaa atctttcgct acaagccagg
      121 tcagcagcct cgttacgaca cgttcactgt tcaggtagaa gacactgccc atgtgatcga
      181 tgccatcgac gctgtttggg ctaagcatga tcacacactc accttccgtc gtgcctgcca
      241 tcattcctcg tgtggttcgt gcgctctgcg cattaatggt gtcgaaaagc ttccctgcat
      301 tacgcgtttt agcgaggtat gggatggatc cagtccactc cgcctcgagc cgctgcgcaa
      361 ctttcccatc gtcagtgacc tggtcgtgga tgtgagcggt tttttttggg ctgaatggca
      421 aacctgggtt gacagaaggt gtagcctgaa aggaaaagga gattaaataa gcacaacagg
      481 agaagaacga tggctcgacg aagcgcacgc cccgaagcgg cgcatgaagt gaccttgacc
      541 ccgttgaaag agcgttgtga gcagtgtggt cagttcttct gggtggccca tcacagccac
      601 cggacggttg ccatgctctc tggtttgtgg agaatgaccc tggtagtacg tcagtgtatc
      661 cagccaagtt gtccgcgctt ccatcagcct taccggccgg aggaagaagg gcgctgggcg
      721 ttgccacacg gcgaatttgg cttggaggtg attgcgctga ttggtcagtg gcgctttcgg
      781 gaacatcgca gtgttccgga gatgcaccag gcacttcagg cacgcggcat gagcattgcg
      841 cagcgcagcg tgacctattt gatgcagcgc tatgaagaac tcgtgacctt gcgcatcacg
      901 gatcaggagc gcatcaaggc acgcttgcag aagcaagggc acgtcatctt ggcgctggat
      961 ggattgcaac cggatgtggg ccacgaagtg ctctgggtgg tgcgtgactg cctctcccaa
     1021 gaaatcctgt tagcccgtcc cttgctcagt agtacgcaag gagacctgac ggctttactc
     1081 aaagaagtca aagctcagtt gaaacagctt aaggtggcgg tcaaaggcgt gatctccgat
     1141 ggcgaagaaa ccattggcag tgccgttgcc tttgtgtttc caggggtggc gcatcaactc
     1201 tgtcagtttc actacttgaa agacgccacc aagctgctct acgaagctga tcgacatgcc
     1261 aaaacgcagt tgaagaagca gctgcgaggc gtgcgaccga tcgagcgtgc attagaagag
     1321 cacagcaccc ccgaaaacga ggcgatccga ggttattgcc tggcggttcg agcctcctta
     1381 accgacgatg ggcgttcacc tttgcagccg tcggggctca agttatacga tcgcatcacc
     1441 caggtgaatg actccattgc ccgtgtccag gaaaaaaaag gttgccaccg gctttaaagc
     1501 tgttgcagca acttctgacc aaaggggtga aggctactgc cgcgctgtgg tccccactgc
     1561 agcgtgcata tggactggtg caccaggcgg cacagatcct agcgaatgag gagcagcaca
     1621 ctggggcaca ggttcgtgag cgctacctgg cacacgtgtg tcagatgcaa gggcagaaag
     1681 cttctctgga accgctgggc gaggccatcg agcatttttg ccacatcacc gacaactttg
     1741 catctgggct ctttgagtgc tatgacgtgg agggcttgcc gcgtaccaac aatgacctgg
     1801 aacattgctt tggggtagca cgagttcatg agcgacgagc tacaggccga cgtggggcca
     1861 ttccaggcgt ggtcgtgcgt ggatccgtcc gcgtgatcgc tgcggtcgtc accaagcagc
     1921 agagcttctc cgccgaggag cttcaacccc gcgattatca gcgctggcgc gacctgcgag
     1981 cgcacttgca acagtgtgag gaaacccgca ggcagcagtt ccgttttcgc aagaatcctg
     2041 ccacttacct ggctgctctc gaagctcaac ttctcaggta aggtttgcca ttcagccaaa
     2101 aaaaccgtta cttcctttat atgaaaccac agtagtccgt cgcccactac ggtcaaggtt
     2161 ttacctggat ttgtgcgaaa ctgctttaca gcattgacaa tgtaatgggc agctccccct
     2221 atcatgacgg atatgtatgc cgttagcaac ccttgtaacg tcagatcttt tgaaaaaaca
     2281 tgaaactgat agaaaagcac tacgagcgcg ccacccaaga taacggctaa gatgttgcca
     2341 aaaatgctag ccgctcgtcg tagcggattt ggcgaggtgc gatagatcag cccattctct
     2401 acatccacta atccctgtac cattaaagga tggtcataca ggtagttcag ggccagagtc
     2461 tgggcctgca atgtcggtat ctcatcggag t
//
LOCUS       DASZAK010001212         2837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_422112, whole genome shotgun sequence.
ACCESSION   DASZAK010001212 DASZAK010000000
VERSION     DASZAK010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422112"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VGT44_21345"
     CDS             complement(<1..401)
                     /locus_tag="VGT44_21345"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEV2583419.1"
                     /translation="MQRMVRTILECMQQAGASRVTNVQLVLGASGHFTVDAAYQHFEA
                     LTKGTPAQDASLTILWLPAQYQCFFCLHRFESSEPSSQVTCPKCGEVALEVGHQDVCF
                     VSAIDVAFQEGEETALPTLITQEEPRIDAHI"
     gene            complement(490..747)
                     /locus_tag="VGT44_21350"
     CDS             complement(490..747)
                     /locus_tag="VGT44_21350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583420.1"
                     /translation="MSNRVSPVKYRVRVTNYSPFRGLEGTIQVVDRVADDPEGPLCFY
                     LIALEGASIPQPVWFDSQEVELIDAPLALQQSQELCGALSA"
     gene            complement(1032..2357)
                     /locus_tag="VGT44_21355"
     CDS             complement(1032..2357)
                     /locus_tag="VGT44_21355"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEV2583421.1"
                     /translation="MHPSLSQHQRHQRRDSGSVLQRFLLILMLLSFSAIMVCVLVLWQ
                     FWQRTPTPGSHPTPSPVVALSPRPSPAPSPTLVPTLTPTADLAQRVDAYIDHLTQAQQ
                     IGQLLMLAVYTNGYTAALNQPLQQWDIANAIVYNQYNGGPLMPTTLSGWTQLVHDLQA
                     HAAQALIIATDEEGGNVDRLAPYYGPTPSPQQLAATGDPRFAYAQAQLDAERLLASGI
                     NADFAPLADVYQGGAIDPSRMFGTTPDQVITYAGAFLDGLQQSGVEGTLKHWPGIGSA
                     SVNPDYGLPTITHSQAELNAIDFAPFRALLSHQPGMIMVTHVMVSAYDPSEPASLSPV
                     LIDQVLRGELGYQGVVVTDAMDGGGLLQFMRQQGYSDPAQAIAEASVRAILAGNDIVE
                     CPMEPDRLAAVVTALTTAVHSGRISQTRLRQSLRRIIALKVKMGLISLP"
BASE COUNT          617 a          801 c          829 g          590 t
ORIGIN      
        1 aagatgtggg catcgatgcg cggctcctcc tgggtgatga gcgtgggaag cgcggtctct
       61 tctccctctt gaaacgcgac atcaatggcg ctgacaaagc acacatcctg gtggccgact
      121 tcaagggcca cttctccgca ctttgggcag gtgacctggc tggatggctc gctgctctcg
      181 aagcggtgca ggcaaaagaa acactggtac tgtgccggca gccacagaat cgtgagcgag
      241 gcgtcttgag cgggcgttcc ttttgtcagc gcctcgaaat gctggtaggc tgcatccaca
      301 gtgaaatgcc ccgatgcgcc cagcaccagc tgcacgttgg tgacgcgaga ggcgccggcc
      361 tgctgcatac attccaggat ggtacgcacc atcctttgca taacggctgc ttcatgcatg
      421 attttcctct ctttccatgc ccgcgctgcg gaactgtgca gcgcaagcag atcacaacct
      481 ataatcaaac tatgccgaaa gagcgccaca cagttcttgc gattgttgaa gggcgagagg
      541 agcatcgatc aactcgacct cctgggaatc gaaccagaca ggctgcggga tagaggctcc
      601 ttctagcgcg atcaaataga agcacaacgg cccttccgga tcgtcggcga cccgatcgac
      661 gacctggatc gtcccttcca gtcccctgaa agggctataa ttcgtgacgc gaacccggta
      721 cttcaccgga gagacgcggt tactcattgc aacttaaact cctttaacgt tggctggcga
      781 aatagcgcga ccctcgcagc aggcgatgga gcattttcct caatagtaag acacacactt
      841 cacaaaaacc tcacagttac cgcgctccgc gtcacgaatc catcacgatt gatttgaaat
      901 cctcatcata aactcatcac catgtttttg ctttcgcagt cgaggggaaa gctgttgaat
      961 gtcacataca atcctatgcg atgtgctggc tggaagccga tattgaactg catggagcgt
     1021 gagcagaggc cctagggcag cgagatcagg cccatcttca ctttgagcgc aatgatgcgg
     1081 cgcagcgact ggcgcaggcg cgtctgcgag atgcgtcctg agtgcacggc agtggtcagg
     1141 gccgtgacca ccgccgccag gcgatccggc tccatcggac attccacgat gtcgttgccc
     1201 gccaggatgg cccgcacgct ggcctcggcg atcgcttgcg ccggatcgct atagccctgc
     1261 tgccgcatga attgaagcag gccaccccca tccatcgcat cggtaaccac aacgccctgg
     1321 tagccgagct cgccgcgcaa gacctgatcg atcagcacgg gcgagagcga ggccggttcg
     1381 gatgggtcat aggcagaaac cataacatgc gtcaccatga tcatccccgg ctgatgcgac
     1441 agcagggcgc gaaacggcgc gaagtcgatg gcgttcagct cggcctggct gtgggttatg
     1501 gtcggcaacc cataatcggg attgaccgat gccgagccaa tacccggcca gtgcttcaag
     1561 gtgccctcga cgccgctttg ctgtaagcca tccagaaatg ctccggcata cgtaatcacc
     1621 tggtcggggg tcgtgccaaa catgcgactc ggatcgatgg ccccgccctg gtagacatcc
     1681 gcgagcggcg caaagtcggc gttgatgcca ctcgccagca ggcgctccgc atccagttgt
     1741 gcctgggcgt aggcgaacct ggggtcaccg gtcgccgcca gttgctgtgg cgaaggcgtg
     1801 gggccgtaat acggagccag gcgatcgacg tttccccctt cctcgtctgt ggcaatgatc
     1861 agcgcctgag cggcgtgcgc ctgaaggtca tgcaccagtt gagtccatcc tgagagtgtg
     1921 gtgggcatca gcggtccccc gttgtactgg ttgtagacga tggcgttggc gatgtcccat
     1981 tgctgtaacg gctggttcag cgccgctgtg tagccattag tatagacggc tagcatgagc
     2041 aactggccga tctgctgcgc ctgtgtcaga tgatcgatgt atgcgtcgac gcgctgcgcc
     2101 agatcggcag tcggcgtaag tgttgggact agtgtgggcg atggagcggg tgagggtcga
     2161 ggagagagcg caacgaccgg ggagggagtc ggatgactcc caggcgtggg tgtgcgttgc
     2221 cagaactgcc acaggacgag cacacagacc atgatcgccg agaaactcag gagcatgagg
     2281 atgagcagga agcgctgcag aacgctgcca gaatctctgc gttgatgccg ctggtgctgt
     2341 gagagggaag gatgcatcgt caaagccgcc tttcgtctcc tcaagagcga ccggcaagcc
     2401 tatcacggcc tttcgatcgc tctgctcgcg cctgtttcgt ccgcaagcgg gtgttacaac
     2461 catttataag gttcaatatt aagtatacac gcttcatctc actgatttat cataggcgct
     2521 gcttctcacc tcccaggcgc atcacagaga agcgcgtctt catttttcct gagaaccaga
     2581 ctttgtgatg ctcctgagag cttgcctcct tgttttgtga agcaattggg atgctttggt
     2641 gatacagaat aagtagagaa acaaagtgaa gttcgggcca aaggagggaa agaaccatga
     2701 ccaacaaatt cactctgcaa gattttgagc aagccgatag tgagcttcgc gccgaggcga
     2761 gccggagaca cttctccatt catgtactcc tgtatgtcgt gaccaatatc gtgttgatca
     2821 ttctcaacat gactgcg
//
LOCUS       DASZAK010001213         6756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_492562, whole genome shotgun sequence.
ACCESSION   DASZAK010001213 DASZAK010000000
VERSION     DASZAK010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492562"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2225)
                     /locus_tag="VGT44_21360"
     CDS             complement(<1..2225)
                     /locus_tag="VGT44_21360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEV2583422.1"
                     /translation="MSVKSSVFKPVAAADRVDYTALEKTIQRWWDEQGTMQQYLHRND
                     QSDRQYSFMDGPMTANNPMGVHHAWGRTYKDLYQRFKTMQGYKQRYQNGFDCQGLWVE
                     VEVEKELGFKSKLDIEAFGIDKFVELCKQRVFTFARRITEQSIRLGYWMDWGNDYYTL
                     SDENNYTIWMFLKRCNEQGLIYKGHDVMMWCPRCATGISNMETLTEDYKETEHLSLYV
                     RFPLVDRPGEYLLVWTTTPWTLAANVAAAVHPDLPYAKVEQDGEFYYLAEELLPILKA
                     LKGRDKGDYHVVETLKGSDMVGWRYRGPFDELPAEQGVEHRVIPWKDVSATEGTGIVH
                     IAPGCGREDFALSKEFDLAVVAPIDEFGVYLEGYDWLTGQEASQVPEAIEKNLAQKGF
                     LYRRQMYRHRYPICWRCKTELIFRLVDEWYISMEQLRYEIMNSVKQVTWIPSFGFDRE
                     MDWLRSMDDWMISKKRYWGLALPIYDCPDCGAFEVIGSEHELKERAVSGWEAFEGHSP
                     HRPWVDAVTIRCKSCGAEVSRIKDVGNPWLDAGIVSFSTLNYRSDRDYWRQWFPADFV
                     TESFPGQFKNWFYSLLVMSTVLEETTPFKTLFGYGTLMGEDGTPMHKSKGNLVVFDDA
                     AERVGSDTMRWLFANHIPEQNLNFPSIPTEAAMEKAVLTGQPVRLSEKWMLVRRTFDK
                     IWNIYSFFVTYANIDGFDPTRHTLAVEDRSELDRWILSELQQTIRALSDGLERYDSAR
                     P"
     gene            complement(2240..2959)
                     /locus_tag="VGT44_21365"
     CDS             complement(2240..2959)
                     /locus_tag="VGT44_21365"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEV2583423.1"
                     /translation="MKTKLLYHENSFTFECSARVVAVEGDEIALDATVFFPGGGGQMP
                     DRGIITWDNRQQEGSVIALNKREDVVWHSLDRMPPPVGTEVVCTIDWDFRYRMMRTHT
                     ALHLLCGIIWKEFGVQVTGGQMYPDKARMDFAMENLNKERIAYIEERVNEAIEADYPI
                     RVYTLPREKAFAIPDLVRTKINLLPPDIDVVRIVEIVGLDLQADGGTHVNHTKEVGGF
                     KITKTENKGKINKRIEIRLTD"
     misc_binding    complement(3014..3330)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3494..6310
                     /locus_tag="VGT44_21370"
     CDS             3494..6310
                     /locus_tag="VGT44_21370"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEV2583424.1"
                     /translation="MNDLLQTFYGPNAGYVLELYERYQQNPATVDSATRAFFENWSPP
                     IESAPSAQQTALPAVPAAIAPAQVAHILAASALAHDIRSRGHLGAHLDPLGSEPMGDP
                     ALLTESHGITDADLTKLPPDIIGGHSAAGASSALEAITNLRAMYSGTISYEFDQVKSP
                     DERQWLRDAVGLRLYRLPDGRDARLNLLRRLTQVESFERFLHTTFPGQKRFSIEGTDV
                     LVPMIDEIIRGAVNTGTREVIIGMAHRGRLNVLAHVLEKPYTTILSEFRHMKHEEGVP
                     LTDTFGFGYTGDVKYHLGAEHQLGEDQGTSVDLKIILSPNPSHLEYVNPVVEGMARAT
                     QERRKHSGSPEQQVNQSLPILIHGDAAFPGEGIVAETLNLWNLKGYRAGGTIHIIANN
                     QLGFTTEPRDYRSTHFASDLAKGFAIPIIHVNADDPEAALMAVRIAHAFRDTFHKDIL
                     VDLVGYRRWGHNEGDEPAFTQPQMYEIIRAHPTVRELYAQRLQDAGVATPDDAATMQK
                     EALQVLEEARREADASEPEPEEANGQNGHTFVEAYPSPLSMETLATYNAALLAWPEGF
                     TPNPKLERLLKRRAETLGPEGGIDWGQAESLAFAAILADGTAIRLTGQDSERGTFSHR
                     HAVLHDQHSGALYIPLQHLPQARAPFSIYNSPLCESGALGFEYGYSVHAPRVLTLWEA
                     QFGDFANVAQVIIDQFIASARAKWKQLSSLVLLLPHGYEGQGPEHSSARLERYLELSA
                     QDNWRVANCSTAAQYFHLLRLQAFNLERNPRPLVVMTPKSLLRHPFAAASAQELAEDH
                     FHDVLDDRDASRRPDAVRRVVLCTGKMAVDLLGNAGQAEDVAIVRVELLYPFPENALK
                     QLLAHYSRAREAVWVQEEPENMGAWYYMAPRLAAMLEPGWTLQHISRPPQSSPATGFS
                     DLFQLEQETIIHQALHTQASVRSA"
     gene            6342..>6756
                     /locus_tag="VGT44_21375"
     CDS             6342..>6756
                     /locus_tag="VGT44_21375"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEV2583425.1"
                     /translation="MSNEVRVPELGESVVSAVIASWLKREGDAVNQGDALVELETDKV
                     NVEVSAEQSGVLQKIIKQVGDEVAIGDVLAVLGDAAGTQETQGQQEQSAQQASPRPVA
                     AAPVTNGGSAPVSPLAQKIAADNNIDLAQVKGSSAH"
BASE COUNT         1365 a         2010 c         1982 g         1399 t
ORIGIN      
        1 ctcggcctgg cgctgtcgta gcgttccagt ccatcgctca aggcgcggat ggtctgctgt
       61 agttcggaga gaatccagcg atccagctcg ctgcggtcct caaccgccag cgtatggcgc
      121 gtcgggtcga agccatcgat attggcgtag gttacgaaaa agctgtagat gttccagatt
      181 ttatcgaacg tgcggcgcac cagcatccat ttctcgctca ggcgcacggg ctggccggtc
      241 agcaccgcct tttccatcgc cgcctcggtc gggatgctgg ggaagttgag attctgctcc
      301 gggatgtggt tggcgaagag ccagcgcatg gtgtcggagc cgacgcgctc ggcggcatcg
      361 tcgaagacca ccaggttgcc cttcgatttg tgcatgggcg tgccatcctc gcccatcagt
      421 gtgccgtagc caaagagcgt cttgaaaggc gtggtctctt ccagcacggt actcatgacc
      481 agtagggagt agaaccagtt tttgaactgg ccggggaacg actccgtgac aaagtccgcc
      541 gggaaccact gtcgccagta gtcgcggtcg ctgcgataat tgagcgtcga gaacgaaacg
      601 ataccggcgt ccagccaggg attgcccacg tccttgatgc gcgagacctc cgctccgcag
      661 ctcttgcagc gaatcgtcac cgcgtccacc caggggcgat gcggtgaatg tccctcgaag
      721 gcttcccagc cggaaacggc gcgctctttt agctcatgtt cgctgccgat tacctcgaag
      781 gccccgcagt cggggcagtc gtagatgggc agcgccagcc cccagtagcg cttcttcgag
      841 atcatccagt catccatgga gcgcagccaa tccatctcgc ggtcgaagcc gaacgaggga
      901 atccaggtga cctgcttgac cgagttcatg atctcgtagc gtaactgctc cattgagata
      961 taccactcat ctaccaggcg gaaaattagc tcggtcttac atcgccagca gatggggtag
     1021 cggtgccggt acatttgcct gcgatagagg aatccctttt gcgccaggtt cttctcgata
     1081 gcctccggca cttgcgaggc ttcctgtccc gtcagccagt cgtagccttc gagatagacg
     1141 ccaaattcat cgatgggggc gacgactgcc aggtcgaact ccttcgagag cgcgaagtct
     1201 tcgcgaccac agccgggggc gatatgcacg atgccagtgc cttcggtggc gctcacgtcc
     1261 ttccagggga tgacgcgatg ctcgacgccc tgctcggcgg gcaactcatc gaatggcccg
     1321 cgatagcgcc agcccaccat gtcgctgcct ttgagcgtct ccaccacgtg ataatcgccc
     1381 ttgtcgcgcc ctttgagcgc cttcaagatg ggcagcagtt cttccgcgag gtagtagaac
     1441 tcaccgtcct gctccacttt tgcgtagggc aggtcaggat gcacggctgc cgcgacattg
     1501 gcagctagcg tccagggcgt ggtcgtccag accagcaggt attcgcccgg gcgatcaacc
     1561 agggggaagc gtacatagag gctgagatgt tcggtttcct tgtagtcctc ggtcagcgtc
     1621 tccatgttgg agatgccagt ggcgcagcgc ggacaccaca tcatcacgtc gtgtcccttg
     1681 tagatcaatc cctgttcgtt gcagcgcttc aggaacatcc agatggtgta gttgttttcg
     1741 tccgaaagcg tgtagtagtc gttgccccaa tccatccaat agcccagccg gatcgattgc
     1801 tcggtgatgc gcctggcgaa ggtgaagacg cgctgcttgc agagttccac gaacttgtcg
     1861 ataccgaagg cttcgatatc gagcttcgat ttaaagccaa gctctttttc gacctcgacc
     1921 tcgacccaca gtccctggca gtcgaagccg ttctggtagc gctgcttgta gccctgcatg
     1981 gttttgaaac gctggtacag gtctttgtag gtgcgacccc aggcatgatg cacacccatc
     2041 gggttgttgg cggtcatcgg accatccatg aacgaatatt gccggtcgct ctggtcgttg
     2101 cggtgcaggt attgctgcat cgttccctgc tcgtcccacc agcgctgaat ggtcttctcc
     2161 agcgcggtat aatctacccg atctgccgcc gccactggtt tgaagacact ggatttaact
     2221 gacacgtcag cctcctcgat caatccgtta gccgaatctc gatccttttg ttgattttgc
     2281 ctttattttc cgtcttggtt atcttgaatc caccgacttc tttggtatga ttgacatgcg
     2341 taccgccgtc cgcctgcaag tccaggccga cgatctctac gatacgcact acatcgatgt
     2401 ctggcggaag caggttgatt tttgtgcgca ccaggtccgg tatggcgaac gccttctcgc
     2461 gcggcagcgt gtagacacgg atgggatagt ccgcctcaat cgcctcattg acccgttcct
     2521 cgatataggc gatgcgctct ttattgaggt tttccatcgc gaagtccata cgcgctttgt
     2581 ccgggtacat ctgcccgcct gtgacctgta cgccgaactc tttccagatg atgccgcaca
     2641 gcaggtgcag cgccgtgtgg gtgcgcatca tgcgatagcg aaaatcccaa tcgatggtac
     2701 acacaacctc cgtgccgact ggtggcggca tgcggtcaag cgagtgccac accacgtctt
     2761 cgcgcttgtt cagggcgatg acgctgccct cttgctggcg gttgtcccag gtaattatgc
     2821 cgcgatccgg catctggccg ccgccgcctg ggaagaacac ggttgcgtcg agcgcgatct
     2881 cgtctccttc gacggcgacc accctggcgc tgcattcaaa tgtgaaactg ttttcgtgat
     2941 agagcagctt cgtcttcatt ggctagctcc tgtaacaaac tacaatcgac tcaaaaaaaa
     3001 tcctctcatc cccgccaggg acgagaggag agaactcccg tggtaccacc ccgattagcc
     3061 acccctgctt gcgcctggct gactcacttg tactcgttgc aacgtacatc ctggcaggtt
     3121 ttccctggta aagtaatgaa gaaccacaaa aagttagaga tgcacatgca atgcctctgc
     3181 gataacggga aggttccggc cagggctacc ttacgcttcg tcctggcggc tcagggatga
     3241 tcttcgactg cctcgctgta cccggtcgca ccagtcccgg actctctttg acagatttgg
     3301 gtagtctact cgttccttcg ttgcctttgc atggctattc taccatagaa tgcgcgttta
     3361 gtctaccatt tcgcaggtag cgcacctggc agacgagatg agcaaatgat tttacgccat
     3421 gccattactt ctaagctgct tccaggtgat aaaatacaac cagataacca cagagcatca
     3481 aggaaattcg cagatgaatg atctattaca aacgttttac ggccccaatg ccgggtatgt
     3541 cctggaactc tacgaacgct accaacagaa tccagcaacg gtagactccg ctactcgtgc
     3601 attctttgag aactggtcgc cccctataga atccgcgcct agtgcacagc agaccgcctt
     3661 gcctgctgtg ccggcggcca tcgcgcccgc tcaggtcgcg catatccttg cagcctcggc
     3721 gctggcgcat gacatccgct cgcgcgggca tcttggcgcg catctcgacc cgctcggcag
     3781 cgagccgatg ggcgacccgg cgctgctcac cgaatcgcat ggcatcaccg acgcggacct
     3841 gacgaagctg ccgccggaca tcatcggggg acactccgcc gcgggggcaa gcagcgcgct
     3901 cgaggctatc accaacctgc gggcgatgta ttcggggacg atcagctatg agttcgacca
     3961 ggtgaaaagc cccgacgagc gccagtggct gcgcgatgcg gtcggcctgc ggctttatcg
     4021 cctgccggat ggccgcgatg cgcgcctcaa tctactcaga cgcctgaccc aggttgagtc
     4081 gtttgagcgc tttctgcata ccacgtttcc ggggcagaaa cgcttttcga tcgaggggac
     4141 cgacgtcctg gttcccatga tcgatgagat cattagaggc gcggtcaaca ccgggacgcg
     4201 cgaggtgatt atcggcatgg cgcatcgggg taggctgaat gtgctggcgc atgtactgga
     4261 aaagccctac acgaccattc tgagcgagtt ccggcatatg aagcatgagg aaggtgtgcc
     4321 gctgactgat actttcggct ttggctatac cggtgacgtg aaatatcacc tgggcgcgga
     4381 gcatcagctt ggcgaggatc agggaacgtc ggttgacttg aaaatcatac tatcgccgaa
     4441 tccgagccat ttggaatacg tgaaccccgt ggtcgagggc atggcgcgcg ccacacagga
     4501 aagacggaag cattccggca gccccgaaca acaggttaat caatcgctgc ccatccttat
     4561 tcatggtgac gccgcttttc ccggcgaggg tattgtcgcg gaaacgctca atctgtggaa
     4621 cctcaaaggc tatcgcgccg gcggcacaat ccatatcatc gccaacaacc agcttggctt
     4681 caccaccgag ccgcgcgatt atcgatcgac gcattttgcc agcgaccttg caaaggggtt
     4741 tgctattccg atcattcacg tcaacgcgga cgatccagaa gcagcgctga tggcggtgcg
     4801 cattgcccat gccttccgcg acacatttca taaagatatc ctggttgacc tggtgggtta
     4861 ccggcgctgg ggtcacaacg aaggcgacga accggccttc acgcagccgc aaatgtatga
     4921 gattatccgt gcgcacccga cggtgcgcga actgtacgcg caacgattgc aggacgcggg
     4981 tgtggcaacg cccgatgacg cggcgaccat gcaaaaagag gcgctgcagg tgctggaaga
     5041 ggccaggcga gaggctgacg cgagcgagcc agagccagag gaggccaacg gtcagaatgg
     5101 ccatacgttc gtcgaggcat acccgtcacc gctctcgatg gagacgctgg caacctacaa
     5161 cgccgcgctg ctggcgtggc cggagggttt cacgccgaat cctaagctcg aacgcctgct
     5221 gaagcgccgc gcggagacgc tggggccgga agggggcatt gattgggggc aggccgaatc
     5281 gctggcgttc gcggcaatcc tggctgacgg aaccgccatc cgtctgacgg gccaggattc
     5341 cgagcgcggc acgttcagcc accgccacgc cgtgctgcac gaccagcata gcggggcgtt
     5401 gtacatcccg ctccagcatc tcccacaggc gcgcgcgccg ttctcgatct acaacagtcc
     5461 gctttgcgaa agcggcgcgc tgggctttga atatggctac agtgttcacg cgccgcgcgt
     5521 cttgacgctg tgggaggcgc agtttggcga cttcgccaac gtcgcgcagg tcattatcga
     5581 tcagttcatc gcctcggctc gcgccaaatg gaagcaactc tcatcgctgg tgttacttct
     5641 gccgcacggc tacgaggggc aggggccgga gcattcgagc gcgcgcctgg aacgctacct
     5701 ggaactgagc gcgcaggaca actggcgcgt cgccaattgc tcgaccgccg cgcaatactt
     5761 ccacctgctg cgcttgcaag ctttcaacct ggaacgcaac ccgcgcccgc tcgtcgtcat
     5821 gacgcccaag agcctgctgc gtcacccgtt cgcggcagcc agcgcgcagg aactggccga
     5881 agaccatttc cacgacgtgc tggatgatcg cgatgcctcg cgccgccccg acgcggtccg
     5941 gcgcgtggtg ctatgtaccg gtaagatggc tgttgacctg ctggggaatg caggccaggc
     6001 cgaagacgtg gcgattgtgc gtgtggagct actttaccct ttccctgaaa acgcactgaa
     6061 acagctattg gcgcactatt ccagggcgcg agaagcggtc tgggtgcagg aagaaccaga
     6121 aaatatggga gcatggtatt acatggctcc gcgcctggca gccatgctgg aaccaggatg
     6181 gacgctgcaa cacatttctc gtccgccgca atctagcccg gccaccggct tttccgatct
     6241 cttccaattg gaacaggaaa ccatcatcca tcaggcgctg catacccagg cgagcgtgcg
     6301 cagcgcataa tattgctatt ttgaaaggta ggaatgtata gatgtccaac gaagttcgcg
     6361 tcccggaact gggcgaatcg gtcgtgagcg ccgtgatcgc gtcgtggctc aagcgcgaag
     6421 gggatgctgt caaccagggt gatgcactgg tagagctgga gactgacaag gtcaatgtgg
     6481 aggtcagcgc ggagcagagc ggtgtgctgc aaaagatcat caagcaggtg ggcgacgagg
     6541 tggcaattgg cgatgtgctg gccgtgctgg gagatgctgc cgggacgcag gaaacgcaag
     6601 gccagcagga acagtcggca cagcaggcat cgccgcgccc tgtagccgcg gcccccgtga
     6661 caaatggggg gagcgcgcct gtttcgccgc tggcgcagaa aatcgccgcc gataacaaca
     6721 tcgacctggc gcaggtcaag ggcagcagcg cgcatg
//
LOCUS       DASZAK010001214         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_706491, whole genome shotgun sequence.
ACCESSION   DASZAK010001214 DASZAK010000000
VERSION     DASZAK010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706491"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VGT44_21380"
     CDS             complement(<1..438)
                     /locus_tag="VGT44_21380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583426.1"
                     /translation="MDKQQSNHTYVRFETASVNGATGAITAPLHAVQQAARPTAVRTG
                     ELQGQTNKWVMLAISASATFMTTLDGSIVNIGLPSIARTFNTAISGSTEWIIIGYLVV
                     IAATLLTFGRLADMIGRKSVFLTGLVIFILGSALSGLAPSLLLL"
     gene            579..1460
                     /locus_tag="VGT44_21385"
     CDS             579..1460
                     /locus_tag="VGT44_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV2583427.1"
                     /translation="MELRHLRYFVTVAEELHFGRAAARLYIVQPSLSQQIRQLEEELG
                     FPLLYRTKRSVELTDAGKVFLVEAKRILAQVQEAKRAAQRANRGEVGRLVIGYISSST
                     YDLLPMMLRLYRERFPEVDVALRELTTREQLRAMEEENIHVGLLRLPISAPLVDIEVV
                     RREPIVCALPEEHPLAVHQRIAVSMLANEPFVLQASQQGAGYYVQLVQLCLASGFSPN
                     VIQEVTEVHTIVSLVAAGMGVSLVPLSARNIRSQGVVYRELEGPATMTEMAAAWLRNS
                     RSTLVQNFLTVAREIAT"
     gene            1738..>3041
                     /locus_tag="VGT44_21390"
     CDS             1738..>3041
                     /locus_tag="VGT44_21390"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2583428.1"
                     /translation="MPTFNDFHLQPATYAALASMEITTPTPVQAEAIPALMAGNDLVG
                     QSATGSGKTLAYGIPLVERLAKDKRSVQALVLVPTRELAIQVNAVLNRLAAGRRLTTA
                     LLVGGRPYGSQMGALRYGSQIVVGTPGRVKDHLDRGTLSLSGLRMCILDEADQMLDSG
                     FAPAIEEILETTPETRQTALFSATIPDWIEKLQQKFLKNPVKVAISTAQGGESTIEQI
                     AYQVPQDQKIAALCTLLNSTEGESSLIFGRTKFGVERLGKQLSKLGFTVGTLHSNKSQ
                     SAREKVLTAFRHGEVQTLLATNVAARGLDIQGIHQVINFELPESSDLFTHRVGRTGRM
                     GRHGKAITLLAPSEVSKWRRMTRTLGHEVTLQRLAIDKQALEIRADAQPEEALENESN
                     ATSQPSRDRKQFSPNQDRGRRERPAYDKYAASVPQRRQNERV"
BASE COUNT          742 a          783 c          883 g          633 t
ORIGIN      
        1 gagcagcagc aaagaaggag cgaggccgga aagcgcagag ccgaggataa aaatgaccag
       61 tcccgtcaag aaaaccgact tcctaccgat catatcggca agtcgaccaa aggtgagcaa
      121 cgtcgcggcg atcacaacga gatacccgat aatgatccat tcggtagagc cgctaatcgc
      181 tgtgttaaag gtgcgcgcaa tgctcggcag accgatattg acaatcgaac catccaatgt
      241 ggtcataaag gttgccgagg ccgatatggc aagcatgacc cacttattcg tctgaccctg
      301 gagttcgccg gtgcggacag cggtgggcct ggctgcctgc tgaaccgcgt gtaatggggc
      361 ggtaattgcg ccggtcgctc cattcacact ggctgtttca aatctcacgt atgtatgatt
      421 gctttgctgt ttgtccatgc tttcctcatt cattgtttca atggtaattg cctcaccacc
      481 tatcccttga ctcaagtata atgtgattac acctgcatgt ccaatacata aatatgaggc
      541 gattaataga tactacctat gagaagggag aagcgagcat ggaactacgt catttacgat
      601 actttgtcac cgtcgcggaa gaactgcatt ttggaagagc ggcagcgcgt ctatacatcg
      661 tgcaaccatc attgagccag cagattcgcc aacttgaaga agagttaggt tttcccctct
      721 tgtatcgaac aaaacgctca gtggaactga cggacgcggg gaaagtcttt ctagtggagg
      781 cgaagcggat ccttgcgcag gtgcaggagg cgaaacgcgc agcgcagcgc gcgaatcgcg
      841 gagaggtagg acggctggtg atcggctata tcagctcaag cacctacgac ctgctgccca
      901 tgatgctgcg cctctatcgc gagcgttttc ctgaagttga tgttgctctg cgagagctta
      961 cgacgcgcga acagttacgc gcgatggaag aggaaaacat tcatgttgga ctactacgct
     1021 tacccatcag cgctccactg gtggatatag aggtggtgcg acgcgaacca atcgtgtgcg
     1081 cgctcccgga ggaacaccca ctggcagtac atcaacgaat tgctgtgtcc atgctggcca
     1141 atgagccgtt tgtgctccag gcgagccagc agggagcggg ttactatgta cagttggtgc
     1201 agttgtgcct tgcgtctggt ttcagcccca atgtgatcca ggaagttacc gaagttcata
     1261 cgattgtgag cctggtcgcc gccgggatgg gcgtctcact ggttcctcta tcagcccgaa
     1321 atatacgaag ccagggtgtt gtctatcgag agttggaagg accggctacg atgactgaga
     1381 tggcggcggc ctggctgcgc aattcccgct caacgcttgt ccagaacttt ctgacggtgg
     1441 caagggaaat cgcgacttga cggattgcgc cggaagaggt ataatgcaag cgttataatt
     1501 gtttgcatgg catggaacag gctgccagat gatctgttcc ctgaataaag acgctaaaga
     1561 gctttgagca atcctcgata gatcacctct tcctgcatac aagtttgcac ctgatatcag
     1621 ctagcagcat tgcttccgtg tcctacggcc tgctcctggt actccctgtg ggcattgggc
     1681 cttgagggag ctgtaccgtg tcctcaaggg gcgcgatggc cgaaaggatt ttttttcatg
     1741 ccaactttta atgatttcca tttgcaaccc gcgacctacg cggcactggc aagtatggaa
     1801 ataaccacgc cgacgcctgt tcaggccgag gccattccgg cgttgatggc cgggaacgac
     1861 ctggtgggac aatcagccac cggctcagga aagacgctcg cctatggtat ccccctggtg
     1921 gagcggttag cgaaagacaa acgcagcgtg caggcgctgg tactcgtgcc aacgcgcgaa
     1981 ctggctattc aagtgaatgc tgtgctgaac aggctggcag ccggtcggcg gcttacaacg
     2041 gcgctgctgg tgggcggacg gccctatggc agccagatgg gggcgttgcg ctatggatcg
     2101 cagattgtgg taggcacccc gggacgagtg aaggaccatc tcgatcgcgg tacgttatcg
     2161 ctgagcggac tgcgtatgtg catactggac gaagcagatc agatgctgga tagtggtttc
     2221 gcgccagcca tcgaggagat actggagacc acgccagaga cgcgacaaac agcgctcttt
     2281 tcggcgacga taccggattg gatcgagaag ctccagcaga agttcctcaa aaatccggtg
     2341 aaggttgcca tttcaaccgc tcaaggcgga gaaagcacga tagaacagat tgcctaccag
     2401 gtgccacaag accagaaaat agcagcgctc tgcaccctct tgaactcgac agagggagaa
     2461 agcagtctga tctttggcag gacgaagttc ggtgttgagc ggttagggaa acagctcagc
     2521 aagctcggct ttacggtggg aacattacac agcaacaaga gccagagcgc gcgagaaaaa
     2581 gtgctgaccg cgttccgcca cggtgaggtg cagacactgc tcgccacaaa cgtagccgcg
     2641 cgtggtctgg atattcaagg aatacatcaa gtcatcaatt ttgaactgcc agaatcgagc
     2701 gatctgttta cccaccgggt tggccgcacg ggcagaatgg ggcgacatgg caaggcgatt
     2761 acgctgctgg ccccgtcgga ggtctctaaa tggaggcgta tgacgcgcac gttaggacac
     2821 gaagtcacct tgcagcgact tgctatcgac aagcaggctc ttgagatacg agcggacgct
     2881 cagccagagg aagcgctaga aaacgagtcg aacgctacgt cgcagccatc gcgtgaccgc
     2941 aaacagttct ccccaaatca ggatcgcggc aggcgtgagc gaccagcgta tgataaatat
     3001 gcggcatccg tcccccaaag gcgacagaac gagcgtgtca g
//
LOCUS       DASZAK010001215         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_387256, whole genome shotgun sequence.
ACCESSION   DASZAK010001215 DASZAK010000000
VERSION     DASZAK010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387256"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1490)
                     /locus_tag="VGT44_21395"
     CDS             complement(<1..1490)
                     /locus_tag="VGT44_21395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2583429.1"
                     /translation="MVEYLYQSAELPTNQHINISTHQFTGLLASKGVHLLKREFTVAD
                     EYVFNQSSPVMWIISHLTRYPRLIIVYMLVCLTTDALVSVIPLQTGAAFNAVLQGDHR
                     GLLVISLEMLGVVLLTSTFDLSSRVFPEFLSKRLARDAREELYVNLLGKSQTFHNRQR
                     VGDIMARAANDMSQLSFMVAPGLDIVFDSYGSLVIALIFIGFINWQLLLVPLLFTAAL
                     LLALRRYSRQLEPVADSMREQFGELNAVLTESVTGVEVVKATAQEEQEKRKFDHNAGL
                     YRDYFVKNGLVQAWYLPPLLLAIAMVAGFLHGLLLVTLHQLSIGGLVSFMSLLTLLHY
                     PTFNSNWAFSQVQLGLAGAQRILAILKEETDLDENEGGYNAVISGEIAFENVSFSYGD
                     TPVLKNVSFRAAPGQTIAIVGQTGAGKSTLTKLINRIYDVSEGRVLVDGVDVRSWSLD
                     ALRSQVSTIEQDIFLFSRSITENIAYGLGQQADQPAIEQAARDAQA"
     gene            1529..1909
                     /locus_tag="VGT44_21400"
     CDS             1529..1909
                     /locus_tag="VGT44_21400"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV2583430.1"
                     /translation="MHDPIEEFLEGYPPEMQVVSRILRARVRDAMPCAREVLFARHNH
                     FAYALSESRADTIVYICPMKEYVRLGFMFGTHLNDPQQLLVGTGKQLRHVKVRTVEEA
                     NMPALRGLVEEAWADAQTHMKKRV"
     gene            complement(1948..>4026)
                     /locus_tag="VGT44_21405"
     CDS             complement(1948..>4026)
                     /locus_tag="VGT44_21405"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEV2583431.1"
                     /translation="LMSYVPSSAIAAHFSLHGPCASFATGCSAGADAIGQAFWAIQEG
                     HADRMLAGGTDSAINASSINVFSVMKALSTRNDAPEQASRPYDAERDGFVMAEGAGVL
                     LLEERHVALARGAYIYAEIIAFASNSNAYHMTALPEDGLPLQLLLRQVTHEASISPQQ
                     IGYINSHGSSTPSNERAETAAYKAAFGPHAYTIPISSTKSMIGHSQGAASAIETIVTA
                     LALDRQLLPPTINQRTPDPECDLDYIPNIARPARLDVALTHSSGFGGVNTALVLARPQ
                     RMNAPAPPNTSRRVVVTGLGIVAPNGIGREAFWRATSRGMVGIQPIQRFPIHDLPVQV
                     AGEVTGFRVEDYLDRKLANRSDRVTHLALVAVAEALRDAGLSLREIDPLRAGTVIANT
                     FGGVEFVIEQIQALYTRGPRAMSAFSAIAWLQGANAGQVSLRHGLQGYCKVPINDSIG
                     GMDALGIAYRAIQRGAADVLIAGGCEAPLHPFVLHTLANANQLAPGDDPAAYRPFDCR
                     ANGLILAEGAGICILEAYDHAMQRGAHIYGEITGYRQTNDALTLHSHPGDGARYAQAI
                     SSAMQEGSLSATDIGYASLDGRALPAADLAEADALMRVFGPDIDTLPVSVPRTMFGHS
                     YAAAGAIDTITALLALECGIIPPTINTSPSPAYRLNLIRESRETALASVLIGGRSMNG
                     VNSALTIRRT"
BASE COUNT          785 a         1035 c         1401 g          805 t
ORIGIN      
        1 tgcgcctggg cgtcgcgggc ggcctgctcg atggcgggct ggtcggcttg ctgaccgagg
       61 ccataggcga tgttttcggt gatggagcgc gagaagagga aaatgtcctg ctcgatcgtt
      121 gagacctgcg agcgcagagc atccaggctc caggaacgaa catccacgcc atcgaccagt
      181 acgcggcctt cactgacatc atagatgcgg ttgatcagct tcgtcagagt gcttttgccc
      241 gcgccggttt gcccgacgat ggcaatggtc tgcccgggcg cggcgcggaa cgagacgttt
      301 ttgaggactg gcgtatcgcc atagctgaag ctgacattct cgaaggcaat ctcgccgctt
      361 atcaccgcgt tgtaaccgcc ctcgttctcg tccaggtcgg tctcttcttt cagaatggcc
      421 aggatgcgct gcgcgccggc cagaccaagt tgtacctggc tgaaggccca gttggagttg
      481 aaggtgggat agtgcagcag cgtcaacagg ctcatgaagg agaccaggcc gccgatggag
      541 agctggtgga gggtgactag cagcaggcca tgtaagaatc cggcgaccat ggcgatagcc
      601 agcagcaaag gcggcaggta ccacgcctgc accaggccgt tcttgacgaa gtaatcgcgg
      661 tagaggccgg cgttatgatc gaatttgcgc ttttcctgct cttcctgcgc ggtcgcctta
      721 acgacctcca cgccagtgac cgattcggtc agaacggcgt tcaattcgcc gaactgctcg
      781 cgcatactat ccgcgacggg ttctagctgg cgtgaatagc gccgcaaggc cagcagcagg
      841 gcggcggtaa aaagcagtgg gaccagcagc aactgccagt tgatgaaacc gatgaagatg
      901 agcgctataa ccaggctgcc gtatgagtca aagacgatgt cgaggccggg cgcgaccatg
      961 aagctcaact gcgacatgtc gttggcggcg cgcgccatga tatcgccgac gcgctggcgg
     1021 ttatggaagg tctggctttt gcccaaaagg ttgacataca gttcttcgcg agcatcgcgc
     1081 gccaggcgtt tgctcaggaa ttccgggaag acacgcgagg acaggtcgaa agtgctggtg
     1141 agcaggacga cgccaagcat ctccaggcta atgaccagca agccgcgatg gtcgccctgc
     1201 aagacggcgt tgaaggctgc gcccgtctgg agcgggatga ccgagacaag cgcatcggtc
     1261 gtcaggcaga ctagcatata gacaataatc aggcgcgggt aacgggtaag atgggatata
     1321 atccacatga ccgggctgga ttggttgaag acatattcgt cggcaaccgt aaattcgcgt
     1381 ttgagcaagt ggactccctt cgaggcgagc aagccggtaa actggtgagt cgatatgttg
     1441 atgtgttggt ttgttggcag ttcagcagat tggtacagat attctaccat atttagggcc
     1501 tggtttgcaa ggcttaaaag gagcatttat gcatgatcca attgaggagt ttcttgaagg
     1561 ttatccgccg gagatgcagg ttgtcagcag gatattgcgc gcgagggtgc gggatgctat
     1621 gccgtgcgcg cgcgaggtgt tgtttgccag gcataatcac tttgcctacg cattgagcga
     1681 gtcgagggca gatacgatcg tttatatttg tccgatgaag gagtatgtgc ggctggggtt
     1741 tatgtttggt acacacctga atgacccgca gcaattgctg gtcggcacgg gtaaacagct
     1801 gcgtcacgtc aaggtgagaa cggttgagga ggcgaatatg cctgcgctgc gtgggctggt
     1861 tgaggaggca tgggcggatg cgcagacgca tatgaagaag agggtgtgat gtgggatgaa
     1921 agcggtcgcc accggaaatc ttatctttta tgttcgcctg attgttagcg cggagttaac
     1981 cccgttcatg ctccttcctc caataaggac actggcgagc gcggtttcgc gagattctcg
     2041 aatgaggttg agtcgatatg ctggggaggg tgaagtattg attgttgggg gaatgatacc
     2101 gcattcgagg gcgagcaggg cggtgatggt gtcgattgcg ccggctgccg cgtagctgtg
     2161 gccgaacatc gtgcgcggaa cgctgacggg gagcgtatcg atgtcggggc cgaagacgcg
     2221 catgagcgcg tccgcttctg cgagatcggc ggcgggcagg gcgcgtccat ccaggctggc
     2281 gtagccgatg tcggtagctg acaggctgcc ttcctgcatg gcgctcgaga tggcctgggc
     2341 gtaacgcgct ccatcgcccg gatgcgaatg gagggtcagg gcatcgttgg tctggcggta
     2401 gccggtgatt tcgccgtaga tgtgcgcgcc gcgctgcatg gcgtggtcgt aggcttccag
     2461 gatgcagata ccagcgcctt cggccaggat cagtccgttg gcgcggcaat cgaaggggcg
     2521 atacgcggcg ggatcgtcgc caggggcgag ctggttggcg tttgccaggg tgtgcagcac
     2581 gaaaggatgc aaaggcgctt cgcagccgcc agcgatcagc acatccgccg cgccgcgctg
     2641 gatggcgcga taggctatgc ctagagcgtc catgccgcca atgctatcat tgataggtac
     2701 cttgcaatac ccctgtaagc catgccgcaa cgagacctgg ccagcgttgg ccccctggag
     2761 ccaggcgatg gcggaaaagg cgctcatggc gcgcggcccg cgcgtataca gcgcctggat
     2821 ttgctcgatc acaaattcta cgccgccaaa ggtattggcg atgaccgtac cagctcgtaa
     2881 ggggtctatc tcgcgcagcg agagaccggc gtcgcgcagg gcttcagcca cggcgaccag
     2941 ggccaggtgg gttacgcggt cggagcggtt cgccagcttg cggtcgaggt agtcttccac
     3001 tcgaaaacct gtgacttcgc cggcgacctg gacgggtaag tcgtggatgg gaaagcgctg
     3061 gatgggctga atgccaacca tgccccggct ggtggcgcgc cagaaggcct ctctgccgat
     3121 gccgttgggc gcgacgatgc ccaggccggt gacaacgacg cggcgtgagg tgttcggtgg
     3181 tgcaggcgca ttcatccttt gtggacgcgc cagcacgagg gcggtgttga cgccgccgaa
     3241 gccgctggag tgggtcaggg cgacatcgag gcgcgcgggg cgagcgatat tggggatgta
     3301 gtccaggtcg cattcgggat cgggcgtgcg ctggttgatg gtggggggaa gtaattgccg
     3361 gtcgagagcc agggcggtca cgatggtttc gatggcgctg gcggctccct gtgaatggcc
     3421 gatcatggac ttggtagagc tgatgggaat agtataggcg tgtgggccga aggcggcctt
     3481 gtacgcggcg gtctcggcgc gctcgttgga gggcgttgaa ctgccgtgcg agttgatata
     3541 accgatctgc tgcggggaaa tactggcttc gtgtgtgacc tggcgcaaga gcagttgcag
     3601 gggcagacca tcctctggca aggcggtcat gtgataggcg ttgctgttgg aggcgaaggc
     3661 gatgatttcc gcgtaaatgt atgcgccgcg cgccagggcc acatggcgtt cttccagcag
     3721 cagcacgccc gcgccctcgg ccattacgaa gccatcacgt tccgcgtcgt aggggcgcga
     3781 agcctgttcg ggcgcgtcgt tgcgcgtgct taacgctttc atgacggaga agacgttgat
     3841 actggaggca ttgatggcgg agtctgtgcc tccggcgagc atccggtcgg cgtggccttc
     3901 ctggatggcc cagaatgcct ggccgatggc gtccgcgcca gccgagcaac cggtggcgaa
     3961 ggaggcacat ggaccatgca ggctgaagtg agcggcgatg gcgctgctgg ggacgtagct
     4021 catcag
//
LOCUS       DASZAK010001216         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_135997, whole genome shotgun sequence.
ACCESSION   DASZAK010001216 DASZAK010000000
VERSION     DASZAK010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135997"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..>2074)
                     /locus_tag="VGT44_21410"
     CDS             complement(4..>2074)
                     /locus_tag="VGT44_21410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEV2583432.1"
                     /translation="WVESRAREKHTPAVDGERLAKGVENDLRQGGALSQSLSGYEERP
                     AQLDMALAVANALTKQHHLLAEASTGTGKSLAYLLPLIRAGEGAIISTANKALQEQLF
                     FKDIPFVQKHIKEFDAALVKGVGNYICLDRLNAERQESLEGQMPGNTLERLWNIVREF
                     ELTISGDIETIGVTVSPELRSRVNMDRDECTWMKCDFYEKCYVRAMKEQAAKAQILVV
                     NHTLLLLDANAEGFILPQRAVTVVDEAHHLEEEATRAFTISINQSQIYGLLALTRLRA
                     HCAAHLQDEARAQAVAAWDELARVAHPGFKGRTKLREPLEEALKLATALDKVALSLEE
                     QRPQKMEGLKGEREELLYNRLVARVTAAASQIRHVFSVKQPDKYIYYVETVALHSRQR
                     TSPLQVSAAPLDVAQMLAANLFTRSHVIATSATLTTVDPNPVDPEANGPTFAYFRKRV
                     GLDREAFPDVQECILPLTFDYEHNALLYLPRHLPVPAYGDSDASLGYTKSIADEMMRL
                     VVASRGRAFLLFSSKRMLDDVLREFHRLPPGANLRLLRQGDMSRIELVKTFRASEGAV
                     LFGLKSFWEGVDIAGEALSLVVIDKMPFDPPDDPVHEARVQMMKERGEDWFNTYVLPQ
                     AVLRLKQGLGRLLRTHEDRGVMAILDTRLHRKNYGRRVLEALPPARRTHDIRAVERFF
                     EEQDAPF"
BASE COUNT          341 a          632 c          680 g          421 t
ORIGIN      
        1 tatttagaat ggggcgtcct gctcctcgaa gaagcgctcc accgcacgga tatcgtgtgt
       61 tcggcgcgcc ggtggcagcg cctccaggac gcgcctgccg tagtttttgc ggtgcaggcg
      121 cgtatcgaga atggccatga cgccgcgatc ttcgtgcgtg cgcagcaggc ggccaagccc
      181 ctgcttgagg cgcagcacgg cctgcgggag tacgtaggtg ttgaaccagt cttcgccacg
      241 ttccttcatc atctgaacgc gcgcctcgtg gacgggatca tcgggcgggt cgaagggcat
      301 cttgtcgatc accaccagcg agagcgcctc cccggcgatg tccacaccct cccagaagct
      361 tttcaggccg aacaggaccg ctccctcgct ggcgcggaag gttttgacca gctcgatgcg
      421 cgacatatcg ccctggcgca gcaggcgcag gttggcgccg ggcggcagac gatgaaattc
      481 acgcagcacg tcgtccaaca tgcgtttgct ggagaagagc aggaaggcgc ggccccgtga
      541 ggccacgacc aaccgcatca tttcatcggc tatggatttc gtatagccaa gcgaggcgtc
      601 gctatcgccg taggcgggaa ccggcaggtg gcgcggcagg tagagcagcg cgttgtgttc
      661 gtagtcgaag gtcaggggca ggatgcattc ctgtacgtcg ggaaatgcct cgcggtcgag
      721 gccgacgcgc ttgcggaagt aggcgaaagt cgggccgttt gcttcgggat cgaccgggtt
      781 ggggtcgacc gtcgtcaacg tggcggaggt ggcgatcacg tgcgagcggg tgaacaggtt
      841 ggcggcgagc atctgcgcga catccagcgg cgcggcggag acctgcaagg gcgaggtgcg
      901 ttgtcggctg tgcagggcaa cggtctccac ataataaatg tatttgtccg gctgcttgac
      961 ggagaagaca tggcgaatct gagaggcggc ggcggtcacg cgggcaacca gccggttgta
     1021 gagcagttct tcgcgttcgc ccttcaagcc ctccatcttc tgggggcgct gctcttccag
     1081 tgatagcgcc actttgtcca gggcggtcgc cagtttcagc gcttcttcca gcggctcgcg
     1141 cagctttgtg cgtcccttga agccgggatg cgcgactcgc gcgagttcgt cccaggcggc
     1201 caccgcctgc gcgcgcgctt catcctggag atgcgcggcg cagtgcgcgc gcagccgcgt
     1261 cagggcgagc aggccgtaga tctgcgactg gttgatactg atcgtaaagg ctctggtggc
     1321 ttcttcctcc aggtggtgcg cctcgtccac gaccgtcacg gcgcgctgcg gcaagatgaa
     1381 gccctcagcg ttcgcatcga gcagcagcag ggtgtggttg acgaccagta tctgcgcttt
     1441 ggcggcctgt tccttcatgg cgcgcacgta acatttctcg taaaagtcgc acttcatcca
     1501 ggtgcattcg tcgcggtcca tattgacgcg gctgcgcagc tcgggggaga ccgtcacacc
     1561 gatggtttca atatcgccgg agatggtcag ttcaaactcg cgcacgatat tccacaggcg
     1621 ctcaagcgtg ttgcccggca tctgcccttc caggctctcc tggcgttccg cgttcagccg
     1681 gtccaggcag atgtagttgc caacgccttt gaccagcgct gcgtcaaact ccttgatatg
     1741 tttctgcacg aaggggatgt ccttgaagaa tagttgctct tgcagggctt tgttggcggt
     1801 gctgatgatc gctccctccc cggcgcggat gagcggcagg aggtaggcca acgacttgcc
     1861 cgtcccggtc gaagcttccg ccagcaggtg gtgctgcttc gtcagggcat tcgcgaccgc
     1921 cagcgccatg tcaagctggg ctggccgctc ctcgtatccg ctcaggcttt gcgacagcgc
     1981 cccgccctgg cgcaggtcgt tttcgacccc ctttgccaat cgttcgccat ctacagcggg
     2041 ggtgtgcttt tcccgcgccc ggctctcgac ccag
//
LOCUS       DASZAK010001217         3084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_103422, whole genome shotgun sequence.
ACCESSION   DASZAK010001217 DASZAK010000000
VERSION     DASZAK010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103422"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VGT44_21415"
     CDS             complement(<1..375)
                     /locus_tag="VGT44_21415"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEV2583433.1"
                     /translation="MDEIKEQHPHRGDWLREIVFGLNDGLVTTLVFIMAVSEVAPARL
                     LLVVLSEVLAGGVSMALGGYLAARTAKQILDQRIATERYEIEHEPEEERAELVAIYQN
                     KGFTGPLLQRVVSHLTADRDRWH"
     gene            586..2712
                     /locus_tag="VGT44_21420"
     CDS             586..2712
                     /locus_tag="VGT44_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEV2583434.1"
                     /translation="MSLRSLTSIHEHPTLRTIYGACPHDCPDCCALETQVDQQGRAVS
                     VRGRSDHPVTRGWLCAKVNRYLDRVYHPDRLLYPMRRVGPKGSGAFERISWNEAIAEI
                     SGRWRDIIAQHGAQCILPYSYAGTLGLVNGAVTDSRFWNRLGACRLERAICGHAAEEA
                     VMLTIGGRLAPSPEMLIHSKLVLIWGSNPASTAPHIMPFLREAQRNGTRIIVIDPIRT
                     LTARSADQHIQPFPGTDAALALAMMHVIVTEGLHRPDWIAQHTIGWEHLLERIMQFPP
                     ERAAHITGLAAETIIDLACSYATTAPALLRVTDGINRHTNGGQTVRTLACLPAITGQY
                     GLLGGGLMYSTSDWLKWDGLAVTHLHEPTCPPASRTLNMNRLGAILTAEADPPIYSLY
                     VYNANPVASSPNAGKIVEGLMREDLFTVVHELFETDTARYADILLPATSQLEHVDLHK
                     PYGHLSLQYNMPAIAPQGEARSNWDVMRALAAAMGFTESWLQDDANKVIREVLEATAR
                     KTPLLAGITLECLQAEGSIPLAIPSDQQVPFADGIFRTPSGKVEFYSAQAAAKGYDPV
                     PGWEPEVEARTREITTTSPESDARLPLLCPAAHHFVSSTFGNQDCLRTKERAPTLRIH
                     PSDAIPRGIRSGQLVRVSNERGWCRLVAEVTEEVRPGVLATTTVWWPKFSPDQRNVNW
                     TTSDRLADLNGGSTFYTNIVSVEVDG"
     gene            complement(2888..>3084)
                     /locus_tag="VGT44_21425"
     CDS             complement(2888..>3084)
                     /locus_tag="VGT44_21425"
                     /inference="COORDINATES: protein motif:HMM:NF036989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583435.1"
                     /translation="HFTRRDLEALRATGDMLQLNVLGLVSIAEDVSPELALATIDSIE
                     VLGAFRASAAVKAALAGRVR"
BASE COUNT          651 a          951 c          817 g          665 t
ORIGIN      
        1 atgccaacgg tcacggtcgg ccgtcagatg gctgacgaca cgctgtaaga ggggaccagt
       61 gaatcctttg ttctggtaga tggccaccag ttccgcccgt tcctcttccg gttcatgctc
      121 aatctcatag cgctcagtgg cgatgcgctg gtcaaggatt tgcttggcgg tccgtgcagc
      181 cagatagcct cccagcgcca tcgaaacccc tccggccaac acctcactga gcacgaccag
      241 gagtaaacgg gcaggagcaa cctcgctcac cgccatgatg aataccaggg tggtgaccag
      301 gccatcgttg agtccaaaga caatctcgcg cagccaatct cctcggtgag gatgttgttc
      361 tttgatctca tccatatctg ctcctttgtg acgtttcact gctttgtccc tctacccgtt
      421 ctgaaaggtc cctgtttgtc catgcccaca cattatacga taagcactga atcaatgttc
      481 ctcatcctct gcaaaaagtg gcaattggat gattacatat acgcgcctcc tgttgtatag
      541 ttaggaatat ggcagatgat caagtcacta ctgaaaggaa acgacatgtc actcaggtca
      601 ctcacatcca tccacgagca tcctaccctc cgcactatct acggtgcatg cccacacgac
      661 tgccctgact gctgtgcgct ggagacccag gtagaccagc agggacgagc tgtgagtgtg
      721 cgtggtcgct ccgaccatcc agtaacacga ggctggctgt gtgccaaggt caaccgctac
      781 ctggatcgcg tctatcatcc tgatcggctg ctctacccca tgcggcgagt tggccccaaa
      841 ggcagcggcg cgtttgagcg tatttcgtgg aatgaagcga ttgccgaaat cagcggacgc
      901 tggcgtgaca tcatcgccca acatggcgca caatgtatcc tgccctacag ctatgctggc
      961 acactgggac ttgtgaatgg ggccgtcacc gacagtcgtt tctggaatcg gctgggtgcc
     1021 tgtcgattgg aacgcgccat ctgcggccat gccgccgaag aagccgtcat gctgaccatc
     1081 ggtggccgcc tggcaccttc accagagatg ctcatccaca gcaaactggt cctcatctgg
     1141 ggcagcaacc cggccagtac cgcgccacat atcatgccct tcttgcgcga agcccagcgt
     1201 aacggcacac gcatcatcgt catcgacccc attcgcaccc ttaccgcgcg ttctgccgat
     1261 caacatattc aaccattccc tggtacagat gctgcactcg cgcttgctat gatgcatgtg
     1321 atagtgaccg aggggctgca tcgaccagac tggattgcgc agcataccat cggctgggag
     1381 catctcctgg aacgtatcat gcagtttcca cctgagcgtg cagcacacat cacgggtctc
     1441 gccgccgaga ccatcatcga tcttgcttgc agctatgcca ccaccgcacc ggctctcctg
     1501 cgtgtgaccg atggcatcaa ccgccatacc aatggcggtc agactgtccg tactctggcc
     1561 tgccttccgg ccatcacagg gcaatatggc ctgctcggtg gcggattgat gtacagcacc
     1621 agcgattggc tgaagtggga cgggttggcg gtgacacatc tgcacgagcc tacctgtcca
     1681 cctgcgtcgc gtacgctgaa tatgaaccgg ctcggtgcta tcctcaccgc tgaggccgac
     1741 ccacccatct actcactcta tgtctataat gccaatccgg tcgcctcctc gcctaatgca
     1801 ggcaagattg ttgaagggtt gatgcgcgaa gacttgttta cggtcgtgca tgaactcttc
     1861 gagaccgata ctgcccgtta tgccgatatc ctactccctg cgaccagcca gcttgagcac
     1921 gtagacctgc acaaacccta tggtcatctc tccctgcaat acaacatgcc cgctatagct
     1981 ccgcagggtg aggcgcgcag taactgggat gttatgcgtg cgcttgctgc tgctatgggc
     2041 ttcaccgagt cctggttgca agatgatgcg aacaaggtca ttcgtgaggt actggaagct
     2101 acagcgcgta agaccccgct gctcgccggc atcacgctag aatgcctgca ggctgaaggt
     2161 tcaattcccc tggccattcc ctccgaccag caggtacctt ttgccgatgg catcttccgc
     2221 acgccttccg gcaaggtaga attttattct gcgcaggctg ccgcgaaggg atacgatccc
     2281 gtccccggtt gggaacccga agtcgaagca agaaccagag agattactac tacctcgcct
     2341 gagtctgacg cacgtctccc gctcttatgt cctgcggctc atcactttgt cagctcgaca
     2401 tttggcaacc aggattgctt gcggacgaaa gaacgtgccc cgacactgcg catccatccc
     2461 agcgatgcca tcccacgtgg tattcgcagt gggcaattgg tgcgcgtgag caacgagcgc
     2521 ggctggtgtc gcctggtggc cgaagtgaca gaagaagtgc gtcctggtgt gctggcgacg
     2581 acgaccgtct ggtggccgaa gttcagcccc gaccagcgca acgtcaactg gaccacctcc
     2641 gaccgattgg ccgacctcaa cggcggcagc acgttttata ccaacatagt gagtgtagaa
     2701 gtggatggtt agtggccccg gcgctgaacg aggtgtccgc agacgtgctg cgctggtcag
     2761 tcaacaggac gggactttgc tcctcctggg atgctcgttg aaaaagcgta ccatctcggg
     2821 tatgtttctg tgttccctgc cgtggcgaca agttcccaac cctatgcgac cgcagcagaa
     2881 cacatgctca cctgacacgc cccgctaatg cagctttcac tgcggcactg gcccggaagg
     2941 ccccgagcac ctcgatcgag tcaatggtcg ccagagccag ttccggggag acatcctcag
     3001 cgatactaac cagaccaagt acgttgagct gtaacatgtc accagttgca cgcagcgcct
     3061 ccaaatcacg gcgtgtgaag tgtt
//
LOCUS       DASZAK010001218         4645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_671317, whole genome shotgun sequence.
ACCESSION   DASZAK010001218 DASZAK010000000
VERSION     DASZAK010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671317"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="VGT44_21430"
     CDS             <1..691
                     /locus_tag="VGT44_21430"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV2583436.1"
                     /translation="IMSINTPPITPQQYEVLHGGGAEAAEIANQRLSRRRFLRRSLLA
                     VFGLSATASVAGAVDLLYPNLAGQFGAALTLGTKAEFPAARPGDFKINQAGVFYRQEA
                     KSYIVHLAKDTAFSLHGATLADQLAVETIVKDTDGSYWIGLYQRCVHLGCTVPFRDDC
                     VSFKCPCHGSHYHVTGEFLDGPAPRSLDRFALSFNGEDVVIDTGTLNNLVPHPDDTTR
                     LIPVPTVLCSA"
     gene            707..1273
                     /locus_tag="VGT44_21435"
     CDS             707..1273
                     /locus_tag="VGT44_21435"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEV2583437.1"
                     /translation="MRKYDDQAESSNSSAPLHLANDARASRSAQARCKEKVQVGDLTP
                     DFTLLDQASRPVSLGDFLGKTHLVLYFYPKNNTSLCREEACAFRDSYEVFKDAGADVM
                     GVSSDSVESHRQFANEHQLPFTLLSDTDGAIRKRYGVPTAFGLPGRVTYIIDRQGMVR
                     RIFFSQFTSKRHVDEALETLQKIHEEQA"
     gene            complement(1440..2552)
                     /gene="hypE"
                     /locus_tag="VGT44_21440"
     CDS             complement(1440..2552)
                     /gene="hypE"
                     /locus_tag="VGT44_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HEV2583438.1"
                     /translation="MDAPTQNDAERIDDVLKKIERTRQVRRHKFYLRDEKITLSHGSG
                     GKATHNLIEGVFAPAFSNPLLDPMDDAAVFGINGTGQRLAFTTDTYVVSPLFFAGGDI
                     GKLAVHGTINDLAMAGAEPLYLSAGFILEEGFPISDLRRIVASMASAAADAGIAIVTG
                     DTKVVQRGKADGLFINTAGVGIIRATWPLGQTQLQPGDKVLLSGPIGDHGISIMLARE
                     ALEIETDVESDTAPLHSLVAALLAAAGDGVHCLKDPTRGGVATSLNEMALGSEVSIGL
                     DEQAVPIRPGVRGACEILGLDPLTIANEGKLLAIVAPEKAELALAALRAHPLGLEAAI
                     IGTVQAEPPGMVFLRTDIGGMRVLDMLVGDPLPRIC"
     gene            complement(2586..3731)
                     /gene="hypD"
                     /locus_tag="VGT44_21445"
     CDS             complement(2586..3731)
                     /gene="hypD"
                     /locus_tag="VGT44_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HEV2583439.1"
                     /translation="MSMKYVDEYRDADLAKRISEEIAKLSADRTLKLMEVCGGHTHTI
                     YKHGIEDVLPRSIDLVHGPGCPVCVLPMGRIDDAIAIARMDGMIFTTFGDMMRVPGSR
                     GSLLDAKADGADVRFVYSPLDALKLARQHPDRQVVFFAIGFETTAPSTAVTLLRARAE
                     GVKNFSIFCNHVTIIPAIKAILDSPDLRLDGFVGPGHVSMVIGMRPYNFIAREHHKPV
                     VIAGFEPLDIIQSVYMIVKQISEGRAEVENQYARVVRPEGNLKALEAMARTMELRPFF
                     EWRGLGFITHSALKLRSEFADWDAELRYEVPGLRVADPKACQCGEVLKGVIKPWECKV
                     FGTACTPETPIGTCMVSPEGACAAYFNYGRFSMASERTALNILPTTR"
     gene            complement(3846..4541)
                     /locus_tag="VGT44_21450"
     CDS             complement(3846..4541)
                     /locus_tag="VGT44_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV2583440.1"
                     /translation="MMHDSEGSEYDQLFYPFLFEGGKASIEDVLAQVRHSTLEKCREV
                     IALRHTTLESSGEQIVAAGVAMARAFADGATLLAFGNGGSTTDAQDMVTELLNPPFLA
                     WSPLPAIALTSDIAVVTAVGNDVGFDNVFARQIIAHGRRGDIALGISTSGNSINVLVA
                     LEQAKKQGLLTVGLAGYDGGKMMRSKAMDFCINSPSDHIPRIQEAQATVYHALLEVIQ
                     GALILMKNEPSHP"
BASE COUNT         1063 a         1344 c         1239 g          999 t
ORIGIN      
        1 catcatgagt attaatacac cacctattac accacaacaa tatgaagttt tgcatggcgg
       61 aggggccgaa gctgctgaaa tcgcaaatca gcggttgtcg cggcggcgtt tccttcgtcg
      121 atctctgtta gctgtcttcg gactgtctgc aaccgcaagt gtggccggtg cagtcgatct
      181 actctatccc aacctggccg gtcagtttgg agctgccttg acacttggca caaaagcgga
      241 gtttccggcc gccaggccag gtgatttcaa gatcaatcaa gcaggtgttt tctatcgcca
      301 ggaggctaag tcctacatcg tacacctggc taaggataca gcgttctcct tacatggggc
      361 gacgcttgcg gaccagttgg ccgtggagac gatcgtcaaa gatactgatg gttcgtactg
      421 gattggttta tatcaacgtt gtgtccatct aggttgtacc gtcccgtttc gcgatgactg
      481 cgtgagcttc aaatgtccct gtcacggttc gcactatcat gtgaccggtg aattcctgga
      541 cggaccggct cccaggagcc tggatcgctt cgctctttcc ttcaatggag aagatgtggt
      601 cattgatacg ggaaccctca acaacctcgt accgcatcct gacgacacca cacgtttaat
      661 cccagtacca actgtactgt gcagcgcgta aaaaggaggc ctcctaatgc gcaagtatga
      721 cgatcaggca gaatcatcga acagctcggc acctctccat cttgccaacg acgctcgcgc
      781 gtctcgctcc gctcaggcaa gatgcaagga gaaggtgcag gtgggcgacc tgaccccaga
      841 ctttaccctg ctagaccaag caagcagacc agtcagcctt ggggattttc tcgggaagac
      901 ccatctcgtg ctgtatttct atccgaagaa taacacctcc ctctgtaggg aagaggcatg
      961 tgcctttcgg gacagctatg aagtgttcaa ggacgctggc gcggacgtca tgggggtcag
     1021 ctccgattcg gtggagtcac atcgacagtt cgccaacgag caccaactcc ccttcacctt
     1081 gttgagtgac acggatggcg ccatcagaaa gcgctacggc gtgcccacag cgttcgggct
     1141 gccggggcgc gtgacctaca tcatcgacag acagggcatg gtgcgtcgta tcttcttctc
     1201 gcaattcacc tcaaagagac atgtcgatga agcgctcgag acgctgcaaa agatacatga
     1261 ggaacaggcg tagttgtcaa cgtatgcagc caactttcca gcgaaagcac ctggatgact
     1321 ttggctcgcc aattctcagc gctacattga acgtgaaagt cttcttaact gacagtaaag
     1381 ttgatagcca agcccatgat gctcagcaca attcagtgcg tgattacaag gcaaccgtct
     1441 cagcaaatac gaggcaacgg atctccaaca agcatatcca gtacacgcat accaccgata
     1501 tcagtgcgca agaagaccat gccaggaggc tcggcctgga cggtgccgat gattgcagct
     1561 tcaagcccca gtgggtgggc gcgtaaagct gccagcgcaa gctctgcctt ctcgggggca
     1621 acaatggcca ggagtttgcc ctcgttggcg attgtcagag ggtccagacc aagaatctcg
     1681 caggctcctc gcacaccagg gcgtataggg acggcctgct cgtcgagccc aattgaaacc
     1741 tccgagccta aagccatctc attcaacgag gttgctacgc cgccacgtgt agggtctttc
     1801 aggcaatgca cgccgtcgcc ggctgccgcg agcaatgcgg ctaccagcga atggagtggc
     1861 gcggtgtcgc tctccacatc agtttctatt tccagcgcct ctcgagccag catgattgag
     1921 atgccgtggt caccgatggg gccactcaac agcactttgt ccccaggctg caactgagtt
     1981 tgtccaagtg gccaggtggc acggatgatg ccaacgccag cagtgttgat gaacaaccca
     2041 tccgctttgc cgcgctggac caccttcgta tcgcctgtga caatagcgat gcctgcatct
     2101 gccgccgcgc tagccatcga cgcgacgata cggcgcaggt cgctgatagg gaaaccttct
     2161 tccaggataa agccagccga gagatatagt ggttccgcgc cggccatagc aaggtcgttg
     2221 attgtgccat gaacggccag cttcccgata tccccacccg cgaagaaaag cgggcttacg
     2281 acgtacgtgt cagtggtgaa tgccaggcgt tgacctgtcc cgttgatgcc aaacaccgct
     2341 gcatcatcca ttggatcaag cagtgggttt gagaaggcgg gtgcaaagac tccctcaatc
     2401 aggttgtggg tggccttacc gccactccca tgactgagcg tgatcttctc gtcacgcaaa
     2461 tagaatttat ggcggcgtac ctgcctggtg cgctcgattt tcttcagaac atcgtcaatg
     2521 cgttccgcat cgttctgtgt gggtgcatcc atggaaacct cctataaaag ggcgaccgcc
     2581 caagcttatc ttgtcgtcgg aagaatgttc agggcggtgc gctcagaagc catggagaaa
     2641 cggccgtaat tgaagtaggc ggcgcacgct ccctctggcg aaaccatgca ggttccaatg
     2701 ggcgtctcag gcgtacaggc tgtcccgaag accttgcact cccaaggctt gatgactccc
     2761 ttgagcacct caccgcattg acaagccttg gggtcggcaa cacgcagccc aggtacctca
     2821 taacgcaact cggcgtccca gtcggcgaac tctgaacgta acttcaacgc actatgggta
     2881 ataaaaccaa gaccacgcca ctcgaagaaa ggccgcagtt ccatggtgcg agccatcgcc
     2941 tccagcgcct tgaggttgcc ctccgggcgc acgactcgcg catactgatt ctcaacctcc
     3001 gctcgaccct cgctgatctg cttcacgatc atatagacgg actggatgat atcgagtggc
     3061 tcgaaaccgg cgatgaccac aggcttgtgg tgttcgcgcg caatgaagtt gtaaggccgc
     3121 atgccgatga ccatgctgac atgcccaggg ccgacaaagc catcgaggcg caggtcgggc
     3181 gagtcgagga tagctttgat cgcgggtatg atggtcacat ggttgcagaa gatcgagaaa
     3241 ttcttgaccc cttcagccct ggcccgcaaa agtgtgacgg ctgtcgaggg cgcggtagtc
     3301 tcaaagccga tggcaaagaa tacgacctgg cggtcgggat gctggcgagc aagcttgagg
     3361 gcatcgagcg gtgaatagac gaagcgcaca tcagcgccat cggctttggc gtccaggagg
     3421 ctgcccctgg aaccaggcac gcgcatcata tcgccgaagg tagtgaagat cattccatcc
     3481 atgcgagcaa tggcaatggc atcatcgatt cgacccattg gcagtacgca aacggggcat
     3541 cctggcccat ggacgagatc tatcgagcgc ggcaacacat cctcgatccc atgcttgtaa
     3601 atagtgtgcg tatgcccgcc gcagacctcc atcagcttga gcgtccgatc tgcgctcaat
     3661 ttagcaatct cctctgagat gcgcttcgcc agatccgcat cgcggtattc gtcaacatac
     3721 ttcatgctca tgtgattgct ccttcattac ttaacgacct gattatgcac actaactttt
     3781 tcatctggat atcgggaaag atgcccaggg ccgataaatc ggcgatgtgc tcgataaatc
     3841 ggcacctacg ggtggctggg ttcatttttc atcagtatga gcgcgccctg gataacctcg
     3901 aggagcgcgt gatagaccgt tgcctgcgct tcctgaatac gcgggatatg atcactcgga
     3961 gagttgatgc aaaaatccat ggctttcgag cgcatcatct tcccgccatc gtaaccggcc
     4021 aaccctactg tcagcagccc ctgcttcttc gcctgctcaa gggccaccag tacattgatc
     4081 gaattgccgc tcgtcgagat acccagggcg atatcacctc tccgcccgtg cgcaatgatc
     4141 tgtcgggcaa aaacattgtc gaatccgacg tcgttcccca ccgccgtgac taccgcgatg
     4201 tcgctagtca gcgcaattgc cggcaaaggc gaccaggcga ggaatggcgg gttaagaagt
     4261 tcggtcacca tgtcctgagc atcggtggtg ctgcctccgt tgccaaaagc aagcagggta
     4321 gctccatcag caaaagctcg cgccattgca acaccggctg caacgatctg ttcgccagaa
     4381 ctttcgagtg tcgtatggcg cagcgctatc acctcgcgac acttctcaag cgtcgagtga
     4441 cgcacctgcg ccagcacgtc ctctatactg gccttgcctc cctcgaagag aaaaggataa
     4501 aataattggt cgtactcaga accttcggaa tcatgcatca ttcgtctcgc tgctcatagg
     4561 ttagcaatcg ccacgcctcc gtgaaccaga agcaaatcgc ccgggtccac actttccaca
     4621 agcgttatat caatctcttc cgttg
//
LOCUS       DASZAK010001219         3076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_103470, whole genome shotgun sequence.
ACCESSION   DASZAK010001219 DASZAK010000000
VERSION     DASZAK010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103470"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /gene="fgd"
                     /locus_tag="VGT44_21455"
     CDS             complement(<1..890)
                     /gene="fgd"
                     /locus_tag="VGT44_21455"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HEV2583441.1"
                     /translation="MAMHPLNFGYKASAEQFGPRQLLDFTTLAEELGFDSVWISDHFQ
                     PWRHTNGHAPFALSWLAAAAERTRRVTLGTSVLTPTFRSQPAIIAQAFGTLGDLYPGR
                     IILGVGSGESLNEVAVTASEWPTAKERLARLREAVTLIRKLWSEEMVTFDGQYYHTRN
                     ATIYDKPAQPVPIYISAGGPVAAKFAGREGDGFICTSGKGDELYRDQLLPAVQEGALS
                     AGRNPSEIEHTIEVKVSFDTDKARAMADTRIWAALALSAEDKVSIHDPREMERKAEEV
                     ADQAHRRWLVSSDPEEHVEQV"
     gene            1085..1900
                     /locus_tag="VGT44_21460"
     CDS             1085..1900
                     /locus_tag="VGT44_21460"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV2583442.1"
                     /translation="MQLPVSTYVIGDVHGQLKKLTRLLQDAQLISDHLTWEAGTTALW
                     FIGDLLDRGPDSIGVLDFVMRLQGEAAAAGGQVGCLLGNHELLMLGAYRFGRRSTGLG
                     SNFIAKWKQNGGNRKDLARLTMQHLDWLAQLPAMAMVEDRLLLHADAPFYIRYGNSIE
                     EVNANIRGLMSRSDALAWEELLEDFARRGVFASRENGEDFARRFLSIFGGQQIVHGHT
                     PISMLTRHALKHIIEPLVYAGGQCINVDGGMFLGGQGFVYQFSPQSLDGSAPG"
     gene            complement(1897..2883)
                     /locus_tag="VGT44_21465"
     CDS             complement(1897..2883)
                     /locus_tag="VGT44_21465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2583443.1"
                     /translation="MPATYNQGSKLDHYEIIRMLGHGGMNHVYRARDLRNQQEVVLKI
                     PNDDLIGNVAVFERYKREAAIGSRLQHPHIQQLLNTDEERNSHYLVVEFIKGRTLREI
                     LDDHPHEPLLQEEALRIIRQVCEALAYAHEHGVFHRDIKPENIMITESGDIKIIDFGI
                     ALLEGARRVTWRGLSGTVGTPDYMSPEQLRGERGTAASDIYAVGIVLYEMLCGHTPFE
                     SENIFAVMNQHISQDPPSILQFNPHISPALATVVMRAVRRDPLKRYKSMREMLHDLEH
                     LEDVAATPYKPDAPVSSRNRRIIIQSTVAILIFFSIATAIGLLLQYLHHAAH"
BASE COUNT          595 a          894 c          917 g          670 t
ORIGIN      
        1 acttgctcta cgtgttcctc cgggtcgctt gagaccagcc agcggcgatg cgcctggtca
       61 gcgacttcct ccgccttgcg ctccatctcg cgcgggtcgt ggatgctcac cttgtcctcg
      121 gctgatagcg ccagcgccgc ccagatgcgc gtgtctgcca tagcccgcgc cttatcggta
      181 tcaaacgaga ctttgacctc gatggtatgt tcgatctcgg aagggttgcg gcccgctgat
      241 aacgcgcctt cttgcacggc tggcagcagc tggtcgcggt acagctcgtc gcctttgccg
      301 gatgtgcaga tgaacccgtc gccctcacgg cccgcaaatt tcgccgcgac gggaccgccc
      361 gcgctgatgt agatgggaac cggctgagcg ggtttgtcat aaatggtggc gttacgtgta
      421 tggtagtact gaccatcgaa cgtcaccatc tcctccgacc agagcttgcg gatcagcgtg
      481 accgcctcgc gcagccgggc caatcgctct ttcgccgtcg gccattcgct agccgtgacc
      541 gcgacctcgt tgagcgactc gccgctcccg acgcccagga tgatgcgacc agggtagagg
      601 tcgcccaggg tgccaaacgc ttgcgcgata atcgcaggtt gggagcggaa ggtaggcgtc
      661 aacacgctgg ttcccaacgt cacgcgcctg gtgcgttccg ccgccgccgc cagccaggaa
      721 agcgcgaagg gggcatgtcc gttagtatga cgccagggct ggaaatgatc gctgatccag
      781 acgctatcga agccgagttc ttcggccagt gttgtgaaat ccagcagttg gcgcgggcca
      841 aattgctctg ccgaggcttt atatccaaaa ttcagtgggt gcattgccat cgttgttctc
      901 tctatctttc tcattagcac cctctaggag ccggtttacc gtgctcctag aggggtttcc
      961 tttgctcctc tccattttcg cagcaaatgg ggtctttggc aaggtgaaca ggatttgcaa
     1021 aaatgccgcg atgctggtac tgttaaataa aggcaatcat ccacaacagg gaaaacaaca
     1081 ccacatgcaa ttacccgttt ccacctacgt gattggcgat gtacacggcc aactgaaaaa
     1141 gcttaccagg ctgctgcaag atgcgcagct catctccgat catctcacgt gggaagcggg
     1201 tacgaccgcg ctctggttta tcggcgacct gcttgatcgc ggacccgaca gtatcggcgt
     1261 gcttgacttc gtgatgcgct tgcagggtga agctgcggca gccgggggac aggtgggctg
     1321 tctgctgggc aatcacgaac tcctcatgct gggcgcgtat cgcttcggca ggcgctcaac
     1381 cgggttgggc agcaatttca tcgcgaagtg gaagcagaac ggcggcaatc gcaaagacct
     1441 ggcgcgcctg accatgcagc acctggactg gctggcgcaa ctgcccgcta tggcgatggt
     1501 ggaagaccgg ctgctgctgc acgccgatgc gccgttctac attcgctatg gcaattccat
     1561 cgaggaagtc aatgccaaca ttcgcgggct gatgagccgg agcgacgcgc tggcctggga
     1621 ggaactgctg gaggattttg ccaggcgcgg cgtgtttgcc tctcgcgaga acggcgaaga
     1681 tttcgcccgg cgtttcctct ccatctttgg cgggcagcag atcgtacatg gtcatacccc
     1741 catcagcatg ttgacacgcc acgcgctcaa gcatattatc gagccgctgg tctatgccgg
     1801 cgggcaatgt atcaacgtgg atggggggat gttcctcggc ggccagggct ttgtctacca
     1861 gttctcgcca caatcccttg atgggtctgc gccgggctaa tgcgcggcat ggtgcaggta
     1921 ctgcaagagc aggccgatgg cggtggcaat gctaaagaag atcagaatgg caacggtgga
     1981 ctggatgatg atgcggcgat tcctgctgga aacgggggca tccggtttat aaggtgtcgc
     2041 ggctacatcc tccaggtgtt cgagatcgtg cagcatctcc cgcatactct tgtagcgttt
     2101 cagcgggtcg cgccggaccg cgcgcatcac gaccgtcgcc agcgccggtg aaatgtgcgg
     2161 gttgaattgc aggatcgatg gcgggtcctg cgagatatgc tggttcatca cggcgaagat
     2221 attttcgctc tcgaaaggcg tatgcccgca cagcatttcg tagaggacga taccgaccgc
     2281 gtagatatcg gaagcagccg tgccgcgctc gccccgcagt tgttccgggg acatataatc
     2341 gggggtgccg accgtgccgg aaaggccgcg ccacgtcaca cgtcgcgccc cttccagcag
     2401 ggcaatgccg aagtcgataa ttttgatatc gccgctttcg gtaatcatga tgttttccgg
     2461 cttgatgtcg cgatggaaga cgccatgctc atgcgcgtag gccagcgcct cgcagacctg
     2521 gcggataatg cgcagcgctt cctcctggag cagcggctca tgcgggtgat cgtccaggat
     2581 ttctcgcaga gtacgccctt tgatgaattc aacgacgagg taatggctgt tgcgctcctc
     2641 gtccgtgttc agcaactgct gaatgtgcgg atgttggagg cggctgccga tcgccgcctc
     2701 gcgtttgtag cgctcgaaga ccgcgacgtt gccgattaaa tcatcgttgg gaattttcag
     2761 cacaacctcc tgctggttac gcaggtcgcg cgcgcgatac acgtgattca tgccgccatg
     2821 tcccaacatg cggataatct cgtaatggtc tagcttactt ccctggttgt acgttgccgg
     2881 catagacgct gcccattcac tccatgttca aatagcgctg ctcaccatct accatagtat
     2941 atcttttcta agccattgtc tgccaaccgg ccactcgtaa gagggcagat gcaaagtcga
     3001 gaaacaagcg tgaccggcat ggcgaccgca agggatcgcc actacgataa cgctgacggt
     3061 cgcctatccc gtcctt
//
LOCUS       DASZAK010001220         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_350275, whole genome shotgun sequence.
ACCESSION   DASZAK010001220 DASZAK010000000
VERSION     DASZAK010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350275"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VGT44_21470"
     CDS             complement(<1..617)
                     /locus_tag="VGT44_21470"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEV2583444.1"
                     /translation="MTERDADRTRGEQPEFTVHQLSVFCTVARHLSYTKAAEALYLSQ
                     PAVSQQVRTLEQTLGLPLFARSGRGIVLILAGQELLQHAERLLALLAETAPVVQEIHS
                     LERGSLLVGASNSAGTYVLPPLLGAFRAQHPRIHVTLTVANRRSVEEQLLTRQLDLAV
                     MSLIERSDQFVVESLMPYELIVVAAASHKLANRSGLTLLDLLKET"
     gene            728..>2123
                     /locus_tag="VGT44_21475"
     CDS             728..>2123
                     /locus_tag="VGT44_21475"
                     /inference="COORDINATES: protein motif:HMM:NF003252.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HEV2583445.1"
                     /translation="MITTETVSQQRNRWASGVIPYAQMGYWQPDYEPKVTDILCAFRI
                     VPQEGVEPIEAAAAVAGESSTATWTVVWTDRLTAYENYQAKAYRVVPVPGTDQYIAYI
                     AYDIDLFEEGSIANLTSSIIGNVFGFKALKSLRLEDMRIPPHYVKTFQGPPHGIVMER
                     EYLDKYGRPLLGATTKPKLGLSARNYGRVVYEALRGGLDFVKDDENINSQPFMRWRDR
                     FLYCMEAVNKAVAETGEIKGHYMNVTAATMEDMYERAEFAKELGSVIVMIDLTIGYTA
                     IQSMSRWARKNGLILHLHRAGHGTYTRQKTHGVSFRVIAKWMRLAGVDHIHAGTVVGK
                     LEGDPMSIRGYYQTLRGAKYDAEPSLGLFFEQDWASMPGVMPVASGGIHAGQMDMLLH
                     YLGEDVVLQFGGGTIGHPQGIAAGATANRVAVEAMIQARNEGRDVAKEGQEILEKAAR
                     WCGPLRAALELWKDI"
BASE COUNT          467 a          602 c          624 g          430 t
ORIGIN      
        1 aaggtctctt taagcaagtc gagcaacgtc agcccggaac ggtttgccag tttgtgagaa
       61 gcggcagcca cgacgatcag ttcatagggc attaaggact caaccacgaa ctggtctgat
      121 cgctcgatca ggctcatcac cgccaggtct agttggcgag tcagcagttg ctcctcgacg
      181 cttcgacggt tggcgaccgt gagtgtgaca tggatgcgcg ggtgttgcgc gcggaacgct
      241 cccagcaacg gcggcaagac atacgttccg gcgctgttgc tcgcgcccac cagcaaactc
      301 ccacgctcca gggagtgaat ctcctgcaca acgggggctg tctccgccag cagagccagc
      361 aaccgttcgg catgttgcag caattcctga ccggccagta tcagcacaat gcccctgccg
      421 ctgcgcgcga aaagtggcag ccccagcgtt tgctcgaggg ttctcacctg ttgcgagacg
      481 gcgggttgag agagatatag cgcttcggcg gctttcgtat aactgagatg gcgcgctact
      541 gtgcagaaca cgctcaactg atgcactgtg aactccggtt gttcgcccct cgttcgatcc
      601 gcgtcgcgtt ctgtcataat gtctatccta tgaacttgat agcaaactgc tactacaact
      661 aataagtata ccatgctaca ctttgctcga aagagagtca catctgaaac tgcgaggagc
      721 gaacagcatg attacaacag aaaccgtatc tcaacagcgt aatcgctggg catcgggagt
      781 catcccgtat gcgcagatgg gctactggca gcctgactac gagccgaaag tcacggacat
      841 cttatgcgcg ttccgtattg tgccgcaaga gggcgtcgaa ccgatagaag ctgcggcagc
      901 cgttgccggc gaatcatcga ccgctacctg gaccgttgtc tggactgacc gtttgaccgc
      961 ctatgagaac taccaggcca aggcgtaccg ggtggttccc gttcccggaa ccgatcaata
     1021 tattgcttac attgcttacg acatcgatct gtttgaagag ggatcgatcg ccaatctgac
     1081 atctagcatc atcggcaacg tctttggctt taaggcgttg aagagcctgc gcctggaaga
     1141 tatgcgcatt ccaccgcact acgtgaagac cttccagggg ccgccgcatg gcatcgtcat
     1201 ggagcgcgaa tacctggata agtatggccg tccattgctc ggcgcgacga ccaagccgaa
     1261 gcttggcctt tccgcgcgca actatggccg tgttgtctat gaagcgctgc gcgggggcct
     1321 ggatttcgtc aaggacgacg aaaatatcaa ctcgcagccc ttcatgcgct ggcgcgatcg
     1381 ctttctctac tgcatggaag cggtcaacaa agctgtggcc gagacgggtg agatcaaggg
     1441 gcattacatg aacgtgaccg ccgcgacgat ggaagacatg tacgagcgag ccgagtttgc
     1501 caaagaactg ggcagcgtca tcgtcatgat cgacctcacg attggctaca ctgccatcca
     1561 gtccatgtcc agatgggcgc gcaagaatgg cctgatcctg cacctgcacc gcgcaggtca
     1621 cggcacctac acgcgccaga agacgcatgg cgtttccttc cgcgttattg cgaaatggat
     1681 gcgccttgcc ggagttgacc acattcatgc cgggacggtg gtcggcaagc tggaaggcga
     1741 tcctatgtca attagaggct attaccagac gctgcgtggc gcgaaatatg atgctgaacc
     1801 gtcgctgggc ctgttcttcg agcaggattg ggcatccatg ccgggcgtca tgccggtcgc
     1861 ttccggcggg attcacgccg gccagatgga tatgctgctg cactacctgg gcgaggatgt
     1921 cgtactccag tttggcggtg gcaccattgg tcacccacag ggcatcgccg ccggagcaac
     1981 cgcgaaccgc gtagcggtag aagctatgat ccaggcgcgc aacgaggggc gcgatgtcgc
     2041 gaaagaaggg caggaaatcc tggagaaggc cgcgagatgg tgcggccccc tgcgcgccgc
     2101 gcttgaactc tggaaggata tca
//
LOCUS       DASZAK010001221         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_422315, whole genome shotgun sequence.
ACCESSION   DASZAK010001221 DASZAK010000000
VERSION     DASZAK010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422315"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            387..749
                     /locus_tag="VGT44_21480"
     CDS             387..749
                     /locus_tag="VGT44_21480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583446.1"
                     /translation="MQTGKLGTGILAGFVAGLTSTIISSLVSLILTLPNLDALRQNLQ
                     QAANQQHVSITYTNSLVIEVLAISLLFSLVLGSLLALAGGAIGGYMGKGSALPPPQEE
                     YREAMFVPPTQQPSEPEP"
     gene            complement(876..1640)
                     /locus_tag="VGT44_21485"
     CDS             complement(876..1640)
                     /locus_tag="VGT44_21485"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2583447.1"
                     /translation="METSLNRSSGQLARRHGEYGFDEPVWPLLFGLLGVIFLVLGFLS
                     FWMFDIPVLGVIWLVCAILFLFTAASYVYTTRRGKFQVWAEILLQLGLRGDEQIIDLG
                     CGRGAVLLMAANLLPRGKATGIDVWKANEQSGNALSVTQQNAQREGVAGSVALHTADM
                     QQLPFPDGSFDLVVSSLAIHNIRKSEGRKQALREAARVLKPGGRLVIADFFTRRYEEC
                     LREFGMTDVTLHAFDWRFWYGGPWAVPRLVSARKPS"
     gene            complement(1698..2165)
                     /locus_tag="VGT44_21490"
     CDS             complement(1698..2165)
                     /locus_tag="VGT44_21490"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEV2583448.1"
                     /translation="MHTHTDLTQALQRTLHTEIPITRHIGLLVVSYDGSRLALSAPIE
                     RNINHKATAFAGSLNSVATLAGWGLIWILLQEAGIEATIVIQDSSSHYRRPITHDFTA
                     SCQKPDEAQVAQFTRMLREKGRARLELQAEIHENDAVAMSFHGRYVAQLHAPS"
BASE COUNT          550 a          667 c          704 g          504 t
ORIGIN      
        1 gcctcgccaa actggaacag gacctgcgct ccatcggcgg tctcggcgga gaggatcaaa
       61 agaagaaaag atagcaggag ggcatggcgc acccttgcgg tcgccatgcc ctcctgccgc
      121 gcccacttgt ttctttgcat aaactagagt aagatgcagc cagacaaatc gcactcaggt
      181 tatcgacata catcattcag gaggcacgca tgaacaacgt tctttcgggc aggcccgccc
      241 tccggcaagg aatcattttc ggtatcatcc tcacagtggt ggaaattgca tttagtttta
      301 tcagtccaag tttaggctcg ttaggtagca tcatagcgct cgcgatttac ctcgttttcg
      361 ggatagtggc aggccgacga gcctcaatgc agactggtaa actgggtacg ggcatcctgg
      421 caggatttgt cgcagggttg accagcacga tcatttcctc gcttgtctca cttatcctga
      481 ctcttcccaa tcttgatgct ctccgccaga acctccaaca ggccgcgaac caacaacatg
      541 tgagcatcac ctatacgaat tcgcttgtca tcgaagtcct ggccatatcc ctgctttttt
      601 cgcttgttct cggctcgctg ctcgcactgg ccggcggcgc aatcggaggc tatatgggta
      661 agggcagcgc gctccctcct ccccaagagg agtaccggga agcgatgttc gttccaccga
      721 cgcaacagcc atccgaaccg gagccgtagg gacgcgatga tgcacatgaa cgaaatactg
      781 cggacagtgg ggtcccttag cctgccccaa accgttctga gagcctggaa ttacgtcaaa
      841 cgaaaatttc cgaccgtgaa gagcagacgc agatctcagg aaggtttgcg tgcgctgacc
      901 agcctgggaa ctgcccaggg gccaccatac cagaagcgcc agtcgaacgc gtgaagggtc
      961 acgtcggtca tcccgaactc gcgcagacat tcctcgtagc gtcgcgtgaa gaaatcggca
     1021 atcacgagcc tgccgccggg tttgagcacg cgtgccgctt cgcgcagggc ctgcttgcgt
     1081 ccctctgact tgcggatatt gtggatcgcc aaacttgaga caaccagatc gaatgagcca
     1141 tcagggaaag gaagttgctg catatctgcc gtgtgcaagg cgacgctccc ggcaactcct
     1201 tcccgttggg cgttctgctg cgtgactgag agagcgttgc ccgattgttc gttcgccttc
     1261 cacacatcaa ttccggtggc cttccccctg ggaagaaggt tggccgccat caagagcaca
     1321 gcgccgcgac cacatcccag atcaattatt tgctcatctc cacgtagccc aagctggagt
     1381 aggatttccg cccacacctg gaatttgccc cggcgcgtgg tgtacacata gctcgccgcg
     1441 gtgaagagaa acaagatggc acacacgagc cagatcacgc cgagaacagg gatgtcaaac
     1501 atccagaaac tcaagaaccc aagaacgagg aagatgacgc cgagcaggcc aaataagaga
     1561 ggccacaccg gctcatcaaa gccatattcg ccgtgtcgac gagcgagttg ccccgacgac
     1621 ctgttgagag aggtttccat cgtagaactc ctttctagat tggtacaagc gaccagtggc
     1681 ccgaaatcct ggaaagccta cgacggggcg tggagttgag cgacgtagcg gccatgaaac
     1741 gacatagcta cggcgtcgtt ctcgtggatt tctgcctgga gttccaggcg cgccctgccc
     1801 ttctcgcgca gcatacgtgt aaattgggcg acctgcgcct cgtcgggctt ctggcatgaa
     1861 gcggtaaaat cgtgcgtaat gggcctgcga tagtggctgg agctgtcttg aataacaatc
     1921 gtcgcctcga tgcccgcttc ttgcaagagt atccagatga gaccccatcc cgccagcgtc
     1981 gccaccgagt tgaggctccc cgcaaacgct gttgccttgt gattaatgtt gcgctcaatc
     2041 ggagcggata acgccaggcg cgagccgtcg tagctgacaa ccagcaagcc gatgtgccgg
     2101 gtgataggga tttccgtgtg gagggtgcgc tgtagagcct gggtgaggtc tgtatgcgtg
     2161 tgcatgatat gtactgtcaa atccttttca tcgttagtgc tttattcagt gtaacacacg
     2221 cttatggagg caaccaggca agggaggatt acaacgacgg ggtttgggtg acgggggtga
     2281 cggtggacgc gggtaaggtc ggcagtgagg gggcgggcag gctagtaggg ttggtgaaaa
     2341 tgagcgtgat ctgcgagacg ttgtagggaa gggtgtctcc gatgccaagt agagagtgaa
     2401 cggagatgga gaccgagttc acgtc
//
LOCUS       DASZAK010001222         1939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_206882, whole genome shotgun sequence.
ACCESSION   DASZAK010001222 DASZAK010000000
VERSION     DASZAK010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206882"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..1288
                     /locus_tag="VGT44_21495"
     CDS             341..1288
                     /locus_tag="VGT44_21495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEV2583449.1"
                     /translation="MHILMLNETEVQRLLDPDALLDELAKGFKAQSSGLVDAPKRIGA
                     SVPNTGFLLAMPAYQQGREVTVKLVSVFHGNKRLGIPSHQALICLFDPQTGTPLSFMD
                     GTYITALRTAGAAALSTRLLARTDTRVLAIVGAGVQGRSHLKMLPRVRPFSEIRIASR
                     HFSDAEQLAAADSRARAVETAEEAVRGADVVCLCTSAGEPVVSLDWLAPGMHVTSVGY
                     APPGGELDRRVIEHGRLFVETRLAFEPPPAGCGELSGLDPSVGTELGEVLLGRSLGRQ
                     SINEITVYKAMGHASEDMAAASLVYHRAKQEGAGRMVVL"
     gene            complement(1318..>1939)
                     /locus_tag="VGT44_21500"
     CDS             complement(1318..>1939)
                     /locus_tag="VGT44_21500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583450.1"
                     /translation="ATGAGKTSLISLISRFYDVQKGAVLVDGVDVRDVRQKDLRQHVG
                     AVLQDPFIFSGTIAGNIRMHEKHISDEQIRQAAEYVNAAHFIELLPHKYNEEVRERGA
                     GLSVGQKQLLAFARAIAFNPEILLILDEATSSVDTETEVLIQDALNKLMLNRTSIIIA
                     HRLSTIRNVDRIIVMHKGRIVEEGTHEQLLNSQGVYRRLYDFQYAN"
BASE COUNT          383 a          522 c          589 g          445 t
ORIGIN      
        1 cagtgcgacg acgacgggcg caagtccgac gggatcaatg acggtgaaga atgtgacgaa
       61 tgcgctaatg gtgaatgtga gtgttgctgc tgctgtcatg gtggcactgt agcactactt
      121 cccctttcaa gtcaagcttg ccattctggc tacagggatt ctcaaaagtc tcttgtggca
      181 tggtcgccta cggcgaccgg gaagagaagc cttgatcaaa cgttagagtt agcttgttat
      241 ggcttcgtat ccgggggtat actggtttga gtcgagcgga gttgggagaa gaacgaggaa
      301 gtggtctcag gtgaagtacg ctgactgcaa ggagggccat atgcacattt tgatgctcaa
      361 tgagacggag gtccagaggc tgttggaccc agacgcgctc ctggacgagc tagccaaggg
      421 tttcaaggcg caaagtagcg gcctcgttga tgccccgaaa cgtattgggg cctccgtccc
      481 gaacacgggg tttctgctcg ccatgccggc ctatcagcag ggccgtgaag tcaccgtcaa
      541 gcttgtctcc gtttttcacg gcaataagcg gctcggcatc ccctcccacc aggccctcat
      601 ctgcttgttc gatccccaga caggtactcc cctctcattc atggacggaa cctacatcac
      661 agcgctgcgc acggcgggcg cggccgccct ctcgaccagg ctgcttgcac gcacggatac
      721 ccgcgtgctt gcgattgtag gcgccggagt gcagggccgc tcgcacctga agatgcttcc
      781 ccgcgtgcgc ccgttctccg agattcgcat tgcttcgcgt cacttctccg acgcggaaca
      841 gctcgcggca gcagattcca gggcacgcgc ggtagagacc gctgaggagg cagtgcgtgg
      901 agccgacgtc gtatgcctat gcaccagtgc aggtgaaccg gtggtaagcc tcgactggct
      961 cgcgcccggg atgcatgtga cctctgttgg ctacgcgcct ccaggaggag aactggaccg
     1021 cagggttatc gagcatgggc gcctctttgt ggagacccgg ctggccttcg agcctccacc
     1081 ggctggttgc ggtgaattgt ctggtctcga tccgagtgtt ggcactgagc tgggcgaggt
     1141 cttgctgggc cggagtttgg gacggcagtc catcaacgaa ataaccgtct ataaggcaat
     1201 gggacacgcc agcgaggata tggctgctgc aagcctggtc tatcaccgcg ctaagcagga
     1261 aggtgctggc cgcatggtgg tgctctaaga tgctggcggg atgggacaag ttgattccta
     1321 atttgcatac tgaaaatcat agagccttcg atatactccc tgactgttca gcaattgctc
     1381 gtgtgtgcct tcttccacga tgcgcccttt gtgcatgacg atgatgcggt cgacattacg
     1441 gatggtcgaa aggcgatggg cgatgatgat cgaggtgcgg ttgagcatca atttgttcag
     1501 agcgtcctgg atcaatactt cggtttcggt gtcaacgctg gatgtcgcct cgtcgaggat
     1561 cagtaggatt tctggattga acgcaatggc cctggcgaaa gcgagcagtt gtttttgccc
     1621 gactgaaagt cccgcgccgc gctcccgcac ttcctcgttg tatttatgag gcagtagttc
     1681 gatgaaatgc gccgcgttta cgtactctgc tgcctggcgt atctgctcat cactgatatg
     1741 cttttcatgc atgcgtatat taccggcaat agtgccggag aagataaacg gatcttgcag
     1801 caccgctccg acgtgctggc gcaggtcttt ttggcgaacg tcgcgcacgt caacaccatc
     1861 gaccaggact gcgcctttct gcacatcgta gaagcgcgag atcaggctga tcagtgaagt
     1921 tttacccgcg cctgttgct
//
LOCUS       DASZAK010001223         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_458974, whole genome shotgun sequence.
ACCESSION   DASZAK010001223 DASZAK010000000
VERSION     DASZAK010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458974"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..883)
                     /locus_tag="VGT44_21505"
     CDS             complement(119..883)
                     /locus_tag="VGT44_21505"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV2583451.1"
                     /translation="MTQTVNGKNGTGTKQSQRLRRPGQRQQERLDRQARRRRRQRLWT
                     SGIAAVAIIALSTFGFIEYQRYTGAQLAAQQATATAQAAARNATATSIANKNATATAV
                     VLSQNCFISSTGVALPAIYASSATPTAGPTTSPALAGTPVTLAGGLKYVDIKVGSGPA
                     AKNNSTVTVEYTGWLASTCQKFDSSYDRSAQSLPVTVGKGQVIKGWDEGLVGMKAGGI
                     RRLYIPASLGYGAAGSSPAIPPNAALIFDVQVLTIK"
     gene            complement(970..2265)
                     /locus_tag="VGT44_21510"
     CDS             complement(970..2265)
                     /locus_tag="VGT44_21510"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR02022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HEV2583452.1"
                     /translation="MKYLAPDYIYAPAGLQTDVAIAIDDDGRIAALTSREAIQHTDAA
                     SVTALPGVALLPGFVNVHSHVFQRALRGHTHRPLSRHDTFWTWRKAMYAEAQRLNPGL
                     LYDMALATSREMLAAGYTSVGEFHYVHHQPGGFPYADANAMSQAVLAAGRDAGMRVVL
                     LMAAYAQSGFDAPPEPEQLRFCDASVELYLDRVESLRSSGVPVGVAPHSVRAVPEAWF
                     RAIASYSQEKRIPLHVHAAEQLAEIEQCQAAYQCGPIELLARYGALGAQTTVVHATHA
                     STREIELLARNGCTVCVCPTTEGDLGDGIAPYAELLAAGIPLAIGSDSNTRLDPIEEL
                     RWAEYTARMRYQRRRVLVADDMASPGPLLLDYGSRMGAAALGIAAGCIAPGMLADFVA
                     IDLRHPALAGWTPQDLLDVLFFGASSQVIVGTWVNGNPV"
     gene            complement(2262..>2678)
                     /locus_tag="VGT44_21515"
     CDS             complement(2262..>2678)
                     /locus_tag="VGT44_21515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583453.1"
                     /translation="TCDVSGASFVVASKAWQMYRAGEADPALFGIFPGGPGGESFIRS
                     GLARDLAALNKQELLCQDAWGLGDLEGGLSEDDLALLDRIANLSSSEHDLAELRAVYE
                     NDNRLRVPKVIKCYTQDGVKKVNVATGEYVEDVRPS"
BASE COUNT          559 a          793 c          829 g          497 t
ORIGIN      
        1 tcactggtcg ctgcccgacc catcaaaggc gacgggcagc gaggaggaac agttggcggt
       61 atatcgccgt gtgcgcgatg ccctacgggc gcgtatcgaa gcagaattgt tgtaacgatt
      121 acttgatggt cagcacctgt acatcaaaga tgagcgccgc gttgggtgga atagctggcg
      181 agctgcctgc tgcgccgtat cccaggctgg ccggaatgta caggcggcga atgccgccgg
      241 ccttcatacc caccagtccc tcatcccagc ccttgatgac ctgccccttg ccaaccgtca
      301 ccggcaagga ttgtgcgctc cggtcatacg agctatcaaa cttctggcaa gtactcgcca
      361 gccagcccgt gtattcgacc gtgacagtac tgttattttt ggcagcaggc ccactgccga
      421 ctttaatatc aacatacttc aacccgccag ccaatgtaac cggcgtaccc gcaagggcag
      481 gcgaggtggt tggaccagca gtcggtgtgg ccgaactggc ataaatcgca ggaagggcca
      541 ctccggtaga agagataaaa cagttctgcg acagtactac agctgtagca gtggcgtttt
      601 tattcgcgat ggaggtggca gtggcattac gcgcggctgc ttgcgcagta gcagtagcct
      661 gctgcgcggc aagctgcgcg ccggtatagc gttgatattc aataaagccg aaggtggata
      721 gggcgataat cgcgacggcg gcaatgccgc tggtccacag gcgctggcgg cggcggcggc
      781 gagcctggcg gtcaagccgc tcctgctggc gctgtcctgg ccggcgcaaa cgctgtgact
      841 gcttcgtccc tgtgccattt tttccgttca cggtttgtgt catacgagtt gagtctccca
      901 attacaatat gatgttgttg aagaattgca tacagatact accatccttg ccgcaatctg
      961 gcaagtagtt catacaggat tcccattgac ccacgttcca acgatgacct gcgacgacgc
     1021 gccaaagaat agtacatcaa gcaggtcttg cggcgtccag cccgccagcg cggggtgacg
     1081 caggtcgatg gcaacgaagt cggccagcat accgggagcg atgcagcccg ccgcgatgcc
     1141 gagtgcggcg gctcccatgc gcgagccata gtcgagcagc agcgggccgg gcgaggccat
     1201 gtcgtctgct accagcacac gacggcgctg gtagcgcatc cgcgccgtat actctgccca
     1261 gcgcagttcc tcaatgggat caaggcgcgt gttgctatcc gagccgatag cgagcggaat
     1321 tccggcagcc agcagttcgg cataaggagc gatgccatcg cccaggtcgc cctcggtggt
     1381 gggacagacg cagacggtac acccattgcg agccagcagt tcgatctcgc gtgtgctggc
     1441 gtgggtggcg tggacgacgg tcgtttgcgc acccagcgcg ccgtaacgag cgagcagttc
     1501 gatggggccg cactggtacg cggcctggca ctgctcgatc tcggcaagct gctcggcggc
     1561 gtgaacgtgc aatgggatac gcttctcctg gctataggaa gcgatggcgc gaaaccaggc
     1621 ttcgggaacg gcgcgcacgg aatggggcgc gacgccgacg ggaacgccgg acgagcgcag
     1681 ggattccacg cggtcgaggt acagctcaac cgaggcgtcg cagaagcgca actgctccgg
     1741 ctccggcggc gcgtcgaaac cgctctgcgc gtaggcggcc atgagcaaga ccacgcgcat
     1801 cccggcgtct ctgcccgccg ccagcactgc ctgcgacatg gcattggcgt cagcgtaggg
     1861 gaagccgccc ggctggtgat gcacgtagtg aaactcgccg accgacgtat agccagccgc
     1921 cagcatctcg cgagaggttg ccagagccat gtcatagagc aggccggggt tgagccgctg
     1981 cgcctcggca tacatggcct tgcgccacgt ccagaaggtg tcatgccggg ataagggcct
     2041 gtgggtatgg ccgcgcaggg cgcgctggaa gacgtggcta tggacattga cgaagccggg
     2101 cagcagcgcg acgccgggca gcgccgtcac gctggcagcg tcagtatgtt gtattgcctc
     2161 tcgcgaagtc agggcagcga tgcgaccatc atcatcgatg gcgatagcta catcggtttg
     2221 caaaccagcg ggagcgtaaa tataatcagg ggccagatat ttcatgatgg acgcacatcc
     2281 tctacatatt cgcctgtcgc aacattcacc tttttcactc catcctgggt ataacacttg
     2341 atgacctttg gtacgcgtaa acggttatca ttttcataca ccgcccgcaa ttcagccaga
     2401 tcatgctctg acgatgagag atttgcgatg cgatccaaca atgccaggtc atcctcggaa
     2461 agaccgcctt ccaaatcgcc cagtccccag gcatcctggc ataagagttc ctgcttattg
     2521 agcgcggcaa gatcgcgcgc caggccggag cgaatgaacg attcgccgcc aggaccgccg
     2581 gggaagatgc caaatagtgc cgggtcggcc tcgccagcgc ggtacatctg ccaggcttta
     2641 ctagcaacaa cgaacgaagc gcctgaaaca tcgcaggt
//
LOCUS       DASZAK010001224         3937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_314756, whole genome shotgun sequence.
ACCESSION   DASZAK010001224 DASZAK010000000
VERSION     DASZAK010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314756"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VGT44_21520"
     CDS             <1..285
                     /locus_tag="VGT44_21520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV2583454.1"
                     /translation="REPAPRREGAAKTMLVMATAHQPGSLHKCLGVLAARNINVLKLE
                     SRPSRNRIWEYVFYLDFEGHRDDPLVRGALADLAGHTTFCKILGSFARNA"
     gene            528..1412
                     /locus_tag="VGT44_21525"
     CDS             528..1412
                     /locus_tag="VGT44_21525"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEV2583455.1"
                     /translation="MIPGEELEPGVENAAVLGLGLIGGSFALALRRNDLARHIAGFDA
                     DPQVTQRAKQRGAITQVCATPEEAVYEADLVVLASPVLAMRELLERIAPALKRGALVT
                     DTASTKALVIEWAESLLPAHAAFVGGHPMAGKEYSGIDSAEAGLFEGCVYCLARAQQT
                     SPGAIARLSGIVERLGAHPYILDAEQHDRLVAGISHLPFLLSSTMVQTLGAAQDWPEM
                     ARLAASGYRDMSRLAAGSPTMYRDICVTNREAILGWLDALEAQLHTLRSYIAINDEAL
                     KDYFAQAKYMRDTAVPRQ"
     gene            1468..2547
                     /gene="aroF"
                     /locus_tag="VGT44_21530"
     CDS             1468..2547
                     /gene="aroF"
                     /locus_tag="VGT44_21530"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV2583456.1"
                     /translation="MIITIRSNVSHDERLQLTALLGNVTGSQRPALSTMLGEQEVIVL
                     DEAKIDAGAGAIIRQQRAVERVVELQTPYQLVSTAFKAESSSIRVGGSNGGHALMIGG
                     DVAPVVMAGPCAVESREQLMTTARAVKAAGAQILRGGAFKPRTSPYQFQGLGIEGLEM
                     LAEAREETGLPVITEVMEPDMVETVAGYADILQIGSRNMQNFPLLYAAGRNSRRRPVM
                     LKRGISATIDEWLLAAEYVMAAGNPNVILCERGIRSFDTKTRNLLDLACVPLLHELTH
                     LPVIVDPSHGTGRSELVPVMSRAGIAAGADGLILEVHPDPDSALCDGRQSITPEQLRS
                     VIAEMRLMNQLLAIKAVQEVPAWVA"
     gene            2570..2959
                     /gene="aroH"
                     /locus_tag="VGT44_21535"
     CDS             2570..2959
                     /gene="aroH"
                     /locus_tag="VGT44_21535"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV2583457.1"
                     /translation="MYCRGIRGATSVEQNEQEEILAATRELLQAIAQQNELQIDDIAS
                     ITFTVTDDLDAVFPALAARQLGWTEVALMCMREIPVPGSLGRCIRVLLHVNTSRGLSE
                     MRHVYLREAVILRPSFALPTDASSFMD"
     gene            2966..>3937
                     /locus_tag="VGT44_21540"
     CDS             2966..>3937
                     /locus_tag="VGT44_21540"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583458.1"
                     /translation="MQKSSSFAIQDRVSIYPCGPLRGALTPPSSKYHTLRYTLAACLA
                     PGTSIIHNPAISDDTEVLLRACGQLGAEIQVAENTLTIRGTGGILRVPQGGSIDVGNA
                     GAVLRLLMGVCVVVPEPVTFVTTYAESLGRRPNADLLAALEQLGAVVQSNSAEGTLPV
                     TVDGRNVRGGKVRVSGKKSSQYISSLLFLAPLLPEGLDIEIADGLTSASFVDLTIDIL
                     RVSGIVVLEEARYRHYIVPGAQTYRPGEYRIPGDYPSAAALLAAAAVTRGDIALTNLP
                     PGDAGGETILKVFAEMGMEITWTGTTIHARAERPLRGITFDGNTAIDS"
BASE COUNT          794 a         1140 c         1236 g          767 t
ORIGIN      
        1 agagaaccgg ccccccggcg cgagggcgcg gccaagacca tgctggtcat ggcgaccgcg
       61 catcaacctg gttcgctaca taaatgtctg ggcgtactgg cggcccggaa catcaacgta
      121 ctcaagctgg agtcgcgccc atcgcgtaat cgcatctggg aatacgtctt ctacctggat
      181 ttcgagggac atcgcgacga cccgctcgtg cgcggcgcgc tggcggacct ggccggccat
      241 acgacttttt gcaaaatact cggctcattc gccagaaacg cataatattc gtcaaaatcg
      301 cttgacagct catgcgtttc ccatgtacaa tacagccaac atgaaaacga tacaggactt
      361 gccaactcaa ctgaatacca cttttagctg gtacttttat tttagccctc cggcctgacg
      421 gagggcctgt tgagttgcgc aacaggccag agagtgaggc cggagaaagc agaggaaaac
      481 acctctgctt ttttgtttgt tcatacttgc atgtaggaga tacaccagtg ataccaggag
      541 aggaacttga gccgggcgtg gagaacgctg ctgtgctggg tctgggttta atcggcggct
      601 cgttcgcgct tgctctgcgc cgcaatgatc tggcgcggca catcgcgggg ttcgacgccg
      661 acccgcaggt gacgcagcgc gcgaagcagc gcggggctat cacgcaggtc tgcgctacgc
      721 cggaggaggc cgtatacgag gccgacctgg tggtattagc ctcgccggtg ctggccatgc
      781 gcgaactgct ggaacgtatc gcgcccgcct tgaaacgggg cgcgcttgtg acggacaccg
      841 ccagcaccaa agcgctggtg atcgaatggg ccgaaagcct gctgcctgcc catgccgcct
      901 ttgtcggcgg acatcccatg gcgggcaagg aatattccgg catcgacagt gcggaggcgg
      961 ggctatttga gggctgtgtc tactgcctgg cgcgggcgca gcagacatct ccaggggcta
     1021 tcgccagatt gagcggtatc gttgaacggc tgggggcgca tccgtacata ctcgacgcgg
     1081 agcagcacga tcgcctcgtg gctggcatca gccatctgcc atttctgctc tcttcgacga
     1141 tggtgcagac cctcggcgcg gcgcaggatt ggccggagat ggcgcggctg gcggcgagcg
     1201 gctaccgcga catgtcgcgg ctggcggctg gcagcccaac catgtatcgt gacatttgtg
     1261 tgaccaaccg ggaagccatc ctggggtggc tcgacgcgct cgaagcgcaa ttacacaccc
     1321 ttcgttccta catcgccatc aatgacgagg cgctgaaaga ctatttcgcg caggcgaaat
     1381 atatgcgtga caccgctgtc ccgcgccagt aaacaacgca acttttgcag ttatcaaatc
     1441 attgatagac ggaaaggacc tcgtagtatg ataatcacca ttcgtagcaa cgttagccat
     1501 gatgaacgtt tgcagttgac agcactgctg ggcaatgtga ccggcagcca gcgcccggcc
     1561 ctctcgacca tgctcggcga gcaagaagtg atcgtgctgg atgaggcaaa gattgatgcc
     1621 ggagctggag ccataattcg ccagcagcga gcagtcgagc gcgtcgtcga gttgcagacc
     1681 ccgtaccagc tcgtcagcac ggcgttcaag gccgaaagca gcagcatccg cgtgggcggc
     1741 agcaacggcg gccacgcgct aatgattggc ggagacgttg cgccggtcgt gatggccggg
     1801 ccgtgcgccg tcgaaagccg ggagcagctc atgaccaccg cgcgtgccgt caaagcggca
     1861 ggcgcgcaga ttttgcgcgg tggggcgttc aaaccgcgca cgtcacccta ccagttccag
     1921 ggattgggca tcgagggact tgaaatgctc gcggaggcgc gcgaggagac tggcctgccg
     1981 gtgattaccg aggtcatgga acctgatatg gtcgagacgg tagcgggcta cgccgatatt
     2041 ttgcagatcg gcagccgcaa tatgcaaaac ttccccctgc tctacgctgc cgggcgcaac
     2101 agccggcgcc gtcccgtcat gctcaaacgt ggcatatcgg ccacgattga tgaatggctg
     2161 ctggcggcgg aatacgtcat ggcggcgggc aaccccaacg tgatcctgtg cgagcgtggc
     2221 atccgcagct tcgataccaa gacgcgcaac ctgctcgacc tggcctgcgt cccactactg
     2281 cacgaactga cgcatctgcc cgtcatcgtt gaccctagtc atggcaccgg gcgcagcgag
     2341 cttgtgccgg tgatgtcgcg cgcaggcatc gcggcaggag ctgacggcct gatcctcgag
     2401 gttcatcccg atccagatag cgcgctgtgc gatggccgcc agtcgatcac gccggagcaa
     2461 ttgcggtcgg ttattgcgga aatgcgcctg atgaaccagt tgctggctat caaagcagtg
     2521 caggaagtcc cggcctgggt agcataaata tcgtacgagg aggagcatca tgtactgccg
     2581 gggcatccgg ggcgcgacaa gcgttgagca aaatgagcag gaggagatac tggcggcgac
     2641 cagggaactc ttgcaggcca tcgcgcagca gaacgagttg cagattgacg atatcgccag
     2701 cataaccttc accgttaccg acgatcttga tgctgtcttt cccgcgctgg ccgcccggca
     2761 gttaggttgg acggaggtcg cgttgatgtg tatgcgcgag attcccgtac cgggcagcct
     2821 gggcagatgt atccgcgtcc tgctgcacgt caacacctcg cgcggcctgt ccgagatgcg
     2881 ccacgtttac ctgcgcgaag cggtaatctt acgaccttcc ttcgcgttgc cgacggatgc
     2941 atcatcgttt atggattaaa agcatatgca aaaatcatcg tcgttcgcga tacaagacag
     3001 ggtgagtatc tatccatgcg gcccattgcg gggcgcgctg acaccgccat catcgaaata
     3061 tcatacattg cgctacactc tggcagcctg tctcgcaccg ggaacgagca ttattcacaa
     3121 tccagccatc agcgacgata cggaagtgtt gttgcgcgcc tgcgggcagt tgggggcgga
     3181 gatacaggtt gcagagaata cgctgacgat acgcgggacg ggcggcatat tgcgtgtgcc
     3241 acagggcggg agtatcgatg ttggcaacgc aggggcggtg ctgcgtctcc tcatgggtgt
     3301 ctgcgtggtt gtaccagagc cagttacgtt tgtgacgact tatgctgaat cgcttggccg
     3361 tcgcccgaac gctgatctgc ttgctgcgct ggaacagctt ggcgcggttg tccagagcaa
     3421 ttcggctgag ggaacgctgc cggtgacggt ggatggaagg aacgtgcgag gcggcaaggt
     3481 gcgggtctcc ggcaagaaga gttcgcagta tatcagctcg ctactcttcc ttgcgccgtt
     3541 gctgccggaa ggattggata tcgagatcgc agacgggctt acttcggcct cgtttgtgga
     3601 cctgaccatc gatattttgc gcgtgtcggg catcgtcgtt ctagaggaag cgcgttaccg
     3661 gcattacatc gtccccggcg cgcagaccta tcgccccggc gagtaccgca tccccggcga
     3721 ttatccatcc gccgccgcgc tgcttgccgc tgccgccgtc acgcggggcg atatcgcgct
     3781 gacaaacctg ccgccgggcg atgctggcgg cgagacaatc ctgaaggttt tcgccgagat
     3841 gggcatggag atcacctgga cgggtacgac tatccacgcg cgcgccgagc ggcctttgcg
     3901 cggcatcacc ttcgatggca acactgccat cgacagc
//
LOCUS       DASZAK010001225         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_706731, whole genome shotgun sequence.
ACCESSION   DASZAK010001225 DASZAK010000000
VERSION     DASZAK010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706731"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VGT44_21545"
     CDS             complement(<1..788)
                     /locus_tag="VGT44_21545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2583459.1"
                     /translation="MEQKVMFEAEKPAEPERESIQESDLNSLAQSDSLRLYLREISRI
                     SLLTAVREASLAERAETGDKDARNSLIEANLRLVVSIAKKYVGQGLSLEDLIGEGNIG
                     LIRAVTKFDYRKGFRFSTYATWWIKQAITRAILEGTRTVRLPVYIMEEVMRVKRMTRQ
                     LYQELGREPTPENIGERLNIPPERVNELLIWAEKVFSLDAPLSEEEENTLGDILEDVR
                     ERGPVEITDQRLLREEIRKVLNQLTMRERQVIELRFGLVDDHDHT"
     gene            1322..3010
                     /locus_tag="VGT44_21550"
     CDS             1322..3010
                     /locus_tag="VGT44_21550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2583460.1"
                     /translation="MTTSMSLMPGALVGGHYVVGALINRGGFGAVYRGIDTSEGNRPC
                     AIKETYDVTPAARRQALMEAAVLFTIDSDHLPKVYDAFEDNGRFYLVMQLIEGQNLLE
                     MMRQRGAPCSEQETLRWLLPIAEVLHELHSRRPAVMHRDIKPGNIIVTPTQTAVLVDF
                     GLTKLYDPNVDTQTNVRAVSEGFSPIEQYRGKTSPQSDIYSLAATMYYLLTGYVAPAA
                     MQRNVRETLIPPRQLNPRISPHVEQALLAALAQNATNRYDSMRDFIAALQPFQSPQQQ
                     LRLAQPSSAPSQPSYNGYADQTFAGSLIENGAAAGPSPAQASAIPSTSPALPASTLAP
                     PPPQSPPLHRPWPARQKPGKGANAPDAPVYPYLPPRPKVAQPAPVIPIYKSLPHPTSQ
                     GCLWGMLQGVLSALLVLSSVVGATLKNPGSIYVAIFMGLFFYIVAGFFTTRKGGSALR
                     GIWAGLWGGISSIVVFWIVFVVGLIIKLEQNIQQHASSAPPIAQASNLQKDLGNIVNQ
                     VASLLFNHSTTQQNNNGLVVYLVVGLVCAMAAGLLGGILGAARFRARMRKKGYI"
     gene            complement(3092..3346)
                     /locus_tag="VGT44_21555"
     CDS             complement(3092..3346)
                     /locus_tag="VGT44_21555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583461.1"
                     /translation="MIRSFPLLASPHEYLRHQLFRPGHNRLAAEEDAHKVQFQHVGFA
                     GEPGRIVDVRTVLLEGVVAAGITWRSGIDRYLEEGAASAA"
     gene            3345..>3619
                     /locus_tag="VGT44_21560"
     CDS             3345..>3619
                     /locus_tag="VGT44_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583462.1"
                     /translation="MTRRYLIFNADCPTCNSLASVIATLADGKIQTMKLDSPEAKELL
                     DQAFPRGWTQQPYLATVKNGRVSVATRYGIVVRLGLLLGPKKSWQAY"
BASE COUNT          748 a         1092 c          984 g          795 t
ORIGIN      
        1 gtgtgatcgt ggtcgtccac caggccaaag cgcagctcga tgacctggcg ttctcgcatc
       61 gtgagctggt tcagcacttt gcggatttcc tcacggagca ggcgctggtc ggtgatttcc
      121 accggcccgc gttcgcgcac atcctcgagg atatcgccga gcgtattttc ctcttcctcc
      181 gaaaggggcg catcgaggga aaagaccttc tcggcccaga tcaacagttc gttcacacgt
      241 tcgggaggga tgttgaggcg ctcgccgatg ttttcgggtg tcggttcgcg gcccagctcc
      301 tggtacaact ggcgtgtcat acgtttgacg cgcatcacct cttccatgat gtagaccggc
      361 aggcgaactg tgcgggtgcc ttccaggatc gcgcgggtga tggcctgttt gatccaccac
      421 gtggcgtagg tggagaagcg aaaccccttg cggtagtcaa acttggtaac ggcgcgaata
      481 agtccaatgt tgccttcgcc gatcaggtct tcgagagaaa gtccctgtcc aacatacttc
      541 ttggcgatgc tcactaccag gcgcaggttc gcctcgatca acgaattgcg cgcatccttg
      601 tctcccgttt ccgctcgttc ggcaagcgat gcttctcgca cggcagtgag gagggagata
      661 cgtgagattt cgcgtaggta gaggcgcaga ctatcgcttt gcgccaggct attcaagtct
      721 gactcctgga tggattcgcg ttctggctcg gctggtttct cggcttcaaa cataactttt
      781 tgttccaatt actcgctctc ttctaccttc gctctcctgc tagcggctca agagatcgca
      841 cacctcctgc tccccgcagc agaatgcatc atagatcggt taacgatggt gtcattgtat
      901 cacacctcct ggttattgca agcatcgatg tgacaggccc gccagtctaa caaccaccag
      961 gctatagacc ataatcggca gcgagcgccc ttttagtaga gataaccaac ttctcaccat
     1021 gttggaacgt agataatggt ggttgttcct atcgttggga cgtgatgatg aactttcccg
     1081 atcaaagccg atcctgtcac cctgagcgca gcgaaggatc tcggtatccg gcgtgtgaga
     1141 cccttcgctg cgctcagggt gacaataaaa gaccgaacga gtccctatga ctgagccact
     1201 tagatttgtt ttacccaata tttgcgcgtc tatgtagaga gaagatcata ttcatcgatc
     1261 ttatgctata ctatggcact atatggcaca tcttgatctt cttttgtaaa ggacttattg
     1321 tatgacgact tctatgtcgt tgatgccggg cgcactcgtc ggggggcatt atgtcgtggg
     1381 cgctctcatc aatcggggag gattcggcgc ggtctatcgc gggattgata ctagcgaggg
     1441 caaccggcct tgcgccatca aggaaaccta cgatgtgacg cccgccgcgc gtcgccaggc
     1501 tctgatggaa gccgccgtcc tcttcaccat cgatagcgac cacctgccaa aggtctacga
     1561 tgccttcgag gataacgggc gcttctacct ggtaatgcag ttgatcgaag ggcaaaacct
     1621 gctggagatg atgcgccagc gcggcgcgcc atgcagcgag caggagacgt tgcgctggct
     1681 gttgcccatc gccgaggtgc tgcacgaact gcacagccgc cgaccggccg tcatgcaccg
     1741 cgacatcaag ccaggcaata ttatcgtcac gccaactcaa acggcggtgc tggtggattt
     1801 tggtctgacc aagctgtacg accccaacgt tgatacacag accaacgtgc gcgccgtatc
     1861 ggaaggcttc agccccatcg agcaatatcg gggaaagacc agcccacagt ccgatatcta
     1921 ctcgctggcc gcgactatgt attacttgct cacaggctat gtagcgccag ccgccatgca
     1981 gcgtaacgtg cgcgagacgc tcattccgcc gcgccagctc aacccgcgca tctcaccgca
     2041 tgtcgaacag gcgctgctgg ccgcgcttgc gcagaatgct accaatcgct atgactcgat
     2101 gcgcgatttc atcgcagcat tgcagccgtt tcaatcgccc caacagcaac tcaggctcgc
     2161 gcagccgtct tcagcgccat cacaaccgtc ctacaacggc tacgctgacc agaccttcgc
     2221 gggtagcctc atagagaacg gcgctgccgc cggaccatcg ccagctcagg ccagcgccat
     2281 tccatcgacc tcacctgcgc taccagcttc tactctcgcg ccgcccccac cgcaatcgcc
     2341 gccactccat cgcccctggc ctgcgcgaca gaagccaggc aagggggcga acgcgccgga
     2401 tgcgccggtc tacccttacc tgccgccgcg cccgaaagtc gcacagcccg cgccggtgat
     2461 cccgatctac aaatcactcc ctcaccccac cagtcagggc tgcctgtggg ggatgctcca
     2521 gggtgtcctc tccgcgctgc tcgtgctatc gtccgtagtg ggcgccacac tgaaaaatcc
     2581 aggcagcatc tacgtggcaa tttttatggg actgtttttc tacatcgtcg ccgggttttt
     2641 cacgacccgt aagggcggca gcgcgctgcg tggtatctgg gccggcctct ggggcggcat
     2701 ctccagtatt gttgtgttct ggatcgtctt tgttgtcggc ctgattatta agttggagca
     2761 gaacatccag cagcatgcga gtagcgcgcc gcccattgct caagcaagta acttgcagaa
     2821 ggatcttggc aacattgtca accaggttgc ctcgctcctg ttcaaccatt cgacgacgca
     2881 acagaataat aatggcctgg tagtatacct ggttgtcggc ctggtctgcg cgatggccgc
     2941 gggtttgctc ggtggcatcc tcggcgcggc acgctttcgc gccaggatgc gcaagaaagg
     3001 atatatctag gtgcgcgcca tcatttcaca cgccctgaaa actcacctgc aacaggtggg
     3061 cagcgacttc ctgacggtga cgctcgaacc cttacgctgc tgaagcggca ccttcttcga
     3121 ggtatcggtc gatacccgag cgccacgtga tgccagccgc tacgactcct tcgagcagga
     3181 cggtacgcac atctactatt ctcccaggct cgcccgcaaa tccgacgtgc tggaactgga
     3241 ctttgtgcgc gtcctcttcc gcagcaagcc gattatgtcc gggccggaag agctggtggc
     3301 gcaggtactc atggggagat gctaacaaag gaaaagagcg gatcatgacc aggcgctatc
     3361 tcatcttcaa tgccgactgc cccacctgta actcacttgc gagcgtcatt gcaaccctgg
     3421 ccgatggaaa aatacagacc atgaaactcg actcgcctga agctaaggag ttgctggacc
     3481 aggcatttcc gcgcggctgg acacaacagc cctatttagc aacggtgaaa aacgggcgcg
     3541 taagtgtagc cactcgttac ggcatcgtcg tgaggcttgg tctcctgctt ggcccgaaaa
     3601 aaagctggca agcctacag
//
LOCUS       DASZAK010001226         2362 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_173246, whole genome shotgun sequence.
ACCESSION   DASZAK010001226 DASZAK010000000
VERSION     DASZAK010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173246"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /gene="serA"
                     /locus_tag="VGT44_21565"
     CDS             complement(<1..1273)
                     /gene="serA"
                     /locus_tag="VGT44_21565"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEV2583463.1"
                     /translation="MNTTHGKGEEAVHILVADRIAQEGIDLLKHELPEAQVDIQPGLS
                     HEQLLARIGMYTALVVRSETQVTADILAAAGKLKIVGRAGVGVDNIDTEAATRQGIMV
                     VNSPTGNIVAAAEHTIAMLMALARHVPSANSSMKAGKWEKSRFLGVEVRHKTLGVIGL
                     GKVGREVAQRAQGLEMQVIAFDPYVSQEHASKLGVTMFSMDEVLQQADFVTLHTSLTS
                     GPQGTRGLIGARELNLLKPGARLINCARGGLIDEEALLHALNENRLAGVALDVFSQEP
                     VRDDTVLRQLLAHERVIATPHLGASTEEAQVGVATDVAEQIVSVLRGGFPRAAVNAPL
                     ILPETLKALEPYMRLVEQMGRLYTQLQPGPLSTVELSCSGEIATYDLRPLQAALIKGL
                     LESVSDAHVNMINAQILARQRGLEIVEQKSPT"
     gene            complement(1293..>2362)
                     /locus_tag="VGT44_21570"
     CDS             complement(1293..>2362)
                     /locus_tag="VGT44_21570"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEV2583464.1"
                     /translation="TPVPLEILEEMTNPVINHRGPEFGAILERVTLRLQYFFQTTSPV
                     LTYASSGTGGQESAIANLFSPGDRVLAISIGVFGNRFASIAEIYGLQVERLECEWGQA
                     ADPAIIEERLQSAAPYKGVLMTHNETSTGVTNDVEQLAALVRRYTPDALILVDAVSSL
                     GCVPLEMDPWGIDVVFTGSQKGWMVPPGLMMIAASPRAWEANKSAKLPRFYFDWELTR
                     KNLEKWQHPVTPPVSLFFALDLALEKMLDEGREAIFERHRSIGEYVRWRAKEMGLQLL
                     ADHAYASNTVTAIYTPAGIDTKALLKDLRVEDGVVLGGGQGKFDGKIFRVGHLGYFEE
                     VDLVEAMDKLERRLRLFGFQK"
BASE COUNT          423 a          786 c          699 g          454 t
ORIGIN      
        1 gcgtgggcga cttctgctcg acgatttcca ggccgcgctg ccgggcaagt atctgcgcgt
       61 taatcatatt gacatgcgca tcggaaaccg attcgagcag ccccttgatg agcgcggcct
      121 gcagcgggcg caggtcgtag gtcgcgattt cgccgctgca cgagagctcg actgtgctga
      181 gcggccccgg ctgcaactgc gtatagaggc ggcccatctg ctcgaccagc cgcatgtagg
      241 gttctagcgc cttgagcgtc tccggcagga tgagcggcgc gttgaccgcc gcgcgcggga
      301 aaccgccgcg cagcacggag acgatctgct cggcaacgtc ggtagccacc ccgacctggg
      361 cctcttcggt cgatgccccc aggtgcggcg tggcgatcac ccgttcgtgc gccagcagtt
      421 gccgcaggac cgtatcatca cgcaccggct cctggctaaa cacatccaac gccacacctg
      481 ccagccggtt ctcgttcagg gcgtgcagca gcgcctcctc gtcgatcaat ccgccgcgcg
      541 cgcagttaat cagccgcgcg cccggcttga gcaggttcaa ttcgcgcgcg ccgatcagcc
      601 cgcgcgttcc ctgcggcccg ctcgtcagcg aggtgtgcag cgtcacaaag tcggcctgtt
      661 gcagcacttc atccatgctg aacatggtga cgcccagctt gctggcgtgt tcctgcgaga
      721 cgtagggatc aaaggcgatc acctgcatct caagcccctg ggcgcgctgg gcgacttccc
      781 ggcccacttt cccaagcccg atgacgccga gcgtcttgtg ccgcacttca acacccagaa
      841 aacggctctt ctcccacttc ccggccttca tgctgctgtt cgcggaggga acgtgccgcg
      901 ccagcgccat caacatagca atggtatgct ctgcagccgc cacgatgttg ccggtggggg
      961 agttgacgac catgatgccc tgccgcgtag ccgcctcggt gtcgatattg tccacgccga
     1021 cgccggcacg cccgacaatc ttcaacttcc cggctgccgc caggatgtca gccgttacct
     1081 gcgtttcgct gcgcactacg agcgcggtat acatgccgat gcgcgccagc agctgctcgt
     1141 gggataaccc cggctggata tcaacctgcg cctccggcag ctcgtgcttg agcaagtcga
     1201 tgccttcctg cgcgatgcgg tcggccacca ggatatgcac cgcttcttcc cccttgccat
     1261 gagttgtgtt catcaccctg cgatcctctc tcctacttct ggaagccgaa caggcgcagt
     1321 cggcgctcca gcttatccat ggcctcgacc aggtctactt cctcgaagta gccgaggtgc
     1381 ccgacccgga agattttccc atcaaattta ccctggccac cgcccaggac aacgccatct
     1441 tcgacgcgca ggtctttgag caacgccttc gtgtcgatgc cagcaggcgt ataaatggcc
     1501 gtgaccgtgt tcgaggcata ggcatgatct gccagcagtt gcaagcccat ttcttttgcg
     1561 cgccagcgca cgtattcgcc gatgctccta tggcgctcga agatcgcctc gcgtccctca
     1621 tccagcatct tctccagcgc gagatcgagg gcgaagaaca gcgaaacggg cggtgtgacg
     1681 gggtgctgcc atttctccaa attcttgcgc gtcagctccc agtcgaagta gaagcgcggc
     1741 agtttcgcgc tcttgttggc ctcccacgcg cgcgggctgg cagcaatcat catcaggccg
     1801 ggcggcacca tccagccttt ctgcgagccg gtgaagacga catcgatacc ccaggggtcc
     1861 atctccaacg gcacgcaccc cagcgagcta accgcgtcaa ccaggatcag cgcgtcggga
     1921 gtgtagcggc gcaccagggc ggcaagctgt tcgacatcgt tggtgacgcc ggtcgaggtc
     1981 tcattatgcg tcatcagcac gcccttgtac ggcgctgccg actgcaagcg ttcttcgatg
     2041 atagcgggat cggcagcctg cccccactcg cactcgaggc gctcaacctg caaaccgtaa
     2101 atctcggcga tgctggcaaa gcggttgccg aagacgccga tcgaaatggc cagcacgcgg
     2161 tcgccgggcg agaagaggtt cgcgatggcg gattcctggc cacccgtgcc ggaagaagcg
     2221 taggtcagga cgggcgaggt ggtctgaaag aaatattgca ggcgcaatgt cactcgttcc
     2281 aggatggccc cgaactccgg tccgcgatgg ttgatgaccg ggttggtcat ctcttccaat
     2341 atctctaatg gcactggcgt gg
//
LOCUS       DASZAK010001227         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_671569, whole genome shotgun sequence.
ACCESSION   DASZAK010001227 DASZAK010000000
VERSION     DASZAK010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671569"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1931
                     /locus_tag="VGT44_21575"
     CDS             <1..1931
                     /locus_tag="VGT44_21575"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEV2583465.1"
                     /translation="VISNPMTRFYDDTSGGIVIGDPLTHGVADLFNGAYDTMLLMLLR
                     FFAHTEETDAELEHLSRATLRLMTTVIRPLGEALTKLPVDRARWPGKTAGPGFGYNRD
                     VHLLPHKPSAWVFFGERLWQLAKLATKLRLNPGLPTEIQEATAALQDLACQFTPSDGS
                     RGVAAKVAELKQMQAELGCSIQASLGGPYLVTNADTLLTWLGERIPARPQMALCRCGG
                     SAIKPFCDGTHARIGFTGQKDPQRVPDRRDTYIGQQVTVLDNRGLCAHAGFCTDRLAS
                     VFLLGQDPFVDPNGARMEEIIRAVRACPSGALSYAIDGREARDQVDQERPPAIEVSRD
                     GPYRITGGIPLKDGQGDDEPRNQGASLEHYSLCRCGHSQNKSFCSGMHWYVDFHDPVA
                     DPDHEPTVFEWVGGLPALTRLTRLFYEKYVQEDPLIGPLFANMSPDHPERVAAWLGEV
                     FGGPKNYTQAYGGYTRMLTQHLGKGLTEEQRARWASLLYQAANEAGLPNDPEFRSAFT
                     SYIEWGSRLAVENSQPGCHPPQNMPVPRWDWGTAGPPGSRVSALSPQNEEESAAFVLP
                     AADEPVGFAQHIKPLFRAMDRQSMEWALDLWSYQDVKSHAAGILQRLQNGSMPCDGAW
                     SQEKVETFQRWVESGMQE"
     gene            2137..2505
                     /locus_tag="VGT44_21580"
     CDS             2137..2505
                     /locus_tag="VGT44_21580"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2583466.1"
                     /translation="MDVTHASAIARAVDQALSTFGRIDVLVNNAGFGLTGAIEEVSDD
                     EGTACMPPMSSGLPHMRDRQSGHIITISSIAGVSGSAGLGAYSASKFAVEGMSEALET
                     PIFWGKTTIPRTRIWDIING"
     gene            2574..>2754
                     /locus_tag="VGT44_21585"
     CDS             2574..>2754
                     /locus_tag="VGT44_21585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583467.1"
                     /translation="MTKEPPIVIEDREELIFMLSEAAALEHMIMCEYLFAAFSLKRDV
                     SEGVTVAQLTAIKGWE"
BASE COUNT          552 a          830 c          852 g          520 t
ORIGIN      
        1 cggtgatctc caacccgatg acgcgcttct atgacgacac ctccggcggc atcgtcatcg
       61 gtgacccatt gacccacggg gtcgccgatc tcttcaacgg tgcatatgac accatgctgc
      121 tcatgttgtt gcgcttcttt gctcataccg aggaaactga tgccgaactc gagcacctct
      181 cacgggccac gctgcggctg atgacgaccg tcattcgccc gctgggcgag gcgctcacga
      241 agctgccggt agatcgtgcc aggtggccag gcaagaccgc gggcccgggc tttggctaca
      301 accgtgacgt tcacctgctg ccgcacaagc cctcggcctg ggtgttcttc ggtgagcgac
      361 tctggcaact ggcgaaactt gcgacgaagc tgcgcttgaa ccctggcctt ccgactgaga
      421 ttcaggaagc cacggccgcg ctgcaagacc tggcctgcca gtttacgccg tcggatggtt
      481 cgcgtggcgt cgcagccaaa gtggccgagt tgaaacagat gcaggccgag ctgggttgtt
      541 ccatccaggc ctcgctgggt ggtccctatc tggtgaccaa tgccgacacc ctactgactt
      601 ggctgggcga gcgcataccg gcgcggcctc agatggcgct gtgccgctgt ggcggctcgg
      661 ccatcaagcc gttctgcgat ggcacccatg cccgcatcgg gttcaccggc cagaaggatc
      721 cacagcgggt ccctgaccgc cgtgatacct atattggtca gcaggtgacg gtgctcgaca
      781 accgcggcct ctgcgctcat gctggcttct gcacggaccg gctggcttcg gtgttcctcc
      841 tcggccagga ccccttcgtc gatcccaatg gtgcacggat ggaggagata atccgcgccg
      901 tgcgagcctg cccctccggc gcgctcagct acgcgatcga cggcagggaa gcccgcgatc
      961 aggtcgacca ggaacgccca cccgcgatcg aggtctccag ggacggcccg tatcggatca
     1021 ccggtggcat cccgctcaag gacgggcaag gcgacgatga gccgcgtaat cagggcgcct
     1081 cgctggagca ctacagcctc tgtcgctgcg gccactccca gaacaaatcg ttctgtagcg
     1141 ggatgcactg gtatgtcgac ttccacgacc cggtggccga cccggatcac gagccaaccg
     1201 tgttcgagtg ggtaggtggc ctacctgcgc tgacgaggct cactcgcctg ttctatgaga
     1261 agtacgtcca agaggaccca ctgattgggc ctctgttcgc caacatgtcc ccagaccacc
     1321 ctgagcgggt ggccgcctgg ctcggagagg tgttcggagg tccaaagaac tatacccagg
     1381 catacggtgg ctatacccgc atgcttaccc agcacctcgg gaaagggctc accgaggaac
     1441 agagggcacg gtgggccagc ctgctctatc aggctgccaa cgaagccgga ctgcccaacg
     1501 atcctgagtt tcgctcggct tttacctcct acatcgagtg gggttcccgg ttggcggtgg
     1561 agaactccca gccaggctgc catcccccgc agaacatgcc ggtaccgcgc tgggactggg
     1621 gcactgcggg gccacccgga tcaagggtct cagcactctc cccccaaaac gaggaagaat
     1681 cagcggcgtt cgtcctgcca gcagccgacg agccggtggg cttcgctcaa cacatcaagc
     1741 cgctgtttcg tgcgatggac cgccagtcga tggaatgggc cctcgacctg tggtcttacc
     1801 aggacgtgaa gagccatgcc gctggcattc tccagcgctt gcagaacggg tcgatgccgt
     1861 gcgacggggc gtggtcccaa gagaaggtgg agactttcca gcggtgggtc gagtccggca
     1921 tgcaggagta gcgagtgtat gagttggaaa tgacaggagt tcaacgatga ctgaaacaca
     1981 acgtgtaagg tttattaccg ggtgctgcag cggatttgga cgtgcccacg cccaggctgt
     2041 gattgcgcat ggcaacaaac tcattgccac tgctcgcaac gtagaaaccc tgcacgatct
     2101 gacggcacag gcactagacc gggccttggg gcggcgatgg atgtcacgca tgcgtcagcg
     2161 atcgccagag ctgttgatca ggcactgtca acctttgggc ggatcgatgt tctggtgaac
     2221 aatgccggct ttggcttgac gggagctatc gaagaggtca gcgatgacga gggcaccgcc
     2281 tgtatgccac caatgtcttc gggcttgccg catatgcgtg accgacagag cggccatatc
     2341 atcactatct catcgattgc cggggtgtcg ggttcagcag gcctgggagc ctatagcgcc
     2401 agcaagttcg ccgtcgaagg gatgtcggaa gcgttggaga ctccaatttt ctggggcaag
     2461 accacgattc ccaggaccag gatttgggat ataataaacg gatagcagat atctgtagag
     2521 gatgccatta tcgccatatc tcatagagaa aacagacaga aggagcaagc accttgacca
     2581 aagaaccccc gattgtgatc gaagaccgcg aagagctcat cttcatgctc tccgaagcgg
     2641 cggcgctgga gcatatgatc atgtgcgaat atctcttcgc cgccttcagt ctgaagcggg
     2701 acgtgagcga gggcgtgaca gtggctcagc tcacggcgat caaagggtgg gagc
//
LOCUS       DASZAK010001228         2010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_314849, whole genome shotgun sequence.
ACCESSION   DASZAK010001228 DASZAK010000000
VERSION     DASZAK010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314849"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            410..1015
                     /locus_tag="VGT44_21590"
     CDS             410..1015
                     /locus_tag="VGT44_21590"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV2583468.1"
                     /translation="MGKIIISENVSLDGVIQDPSGDEGFRHGGWIGRIGDRGREEAAK
                     VLLDEALGTEAMLLGRRSYEFLASRWPSRTGTLADRLNSKPKYVVSSTLQDPAWNNTT
                     VLHGDVVNEISKLKQQINGDIVVAASFQLLHTLLEHDLADELRLMIYPVVLGTGKRLV
                     GETSDKKPMRLVGNKTVAGDLVFLTYEFVWEAKPSVAGEKT"
     gene            1309..1977
                     /locus_tag="VGT44_21595"
     CDS             1309..1977
                     /locus_tag="VGT44_21595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583469.1"
                     /translation="MHNAFVMEGPQPGIAIARSLGDLSHNLYTPVEGLGNLSAASPGE
                     LLFIDYWADPGGMETFFSNPFVQEAADHLYSSREEAEWIPARDAFTFHVPAVVGTTAR
                     FVGMMRAPVRASSDAVTVLSELIWANLRASRRLGHLSHQLFLRHAAVVEARPAANARR
                     SGGEDTALPVEPVEVLAVDFWPTLEGLTEHYRALLSTNGLDEVLAGSLTVSVWEAASG
                     FVAW"
BASE COUNT          434 a          613 c          594 g          369 t
ORIGIN      
        1 ctcgcgccac gccgcgaccc gctttgccct ccaacaccag ctcctctagc tgcatcctgt
       61 tccgctctct cctgcatggc ctcctccctc gcacttcacc gcaccggtgg ccagaccaac
      121 acctttacct aggtaaggtg agagccgtga ggcagtgtcc ggcccccgca tcactcccag
      181 atcggcaagc cgaatacgtt gcattgccga tgtgaaagac agcctgacac ggtagactct
      241 tggtagtgac gcaaggagag acagtacaag ctccgcaatc acggaagctc ttacgagggg
      301 cagcggacgt atcgagcaga ctccttgagt cgctcgatca gtacacttga gtgcacgcag
      361 attcgcagga ggtagtcggt ctgacgacac caacgaaagg agaatcgaaa tggggaaaat
      421 catcatcagc gagaatgtct cgctcgacgg agtcatccag gacccgagcg gtgacgaggg
      481 cttcaggcac ggcggctgga tcggtcgtat tggcgatcgg ggtcgcgaag aggccgccaa
      541 ggtcctgctc gatgaggcgc tgggcaccga ggccatgctg ctgggccggc gcagctacga
      601 gttcttagcc tcgcggtggc catccaggac tggtaccctg gcggacaggc tgaacagcaa
      661 gcccaagtac gttgtgtcct cgaccctcca agatcccgcc tggaacaaca cgaccgtact
      721 acacggtgac gtggtgaacg agatctcaaa gctgaagcag cagataaacg gggacatcgt
      781 cgtcgccgcc agcttccagc tcctgcacac gctgctagag cacgacctcg ccgacgagct
      841 gcggctgatg atctacccag tcgtgctcgg gaccggcaag cgcctcgtcg gcgagaccag
      901 cgacaagaaa cccatgcgcc tcgtcggcaa caagaccgtc gctggcgacc tcgtcttcct
      961 cacctacgag ttcgtctggg aggcaaagcc gagcgtagcc ggcgaaaaaa cctgaaaccc
     1021 gctctgctcg cataaggaag ttcatcgcac tgttagcgaa caaacagaaa ctctacccgt
     1081 agtcaggatt tcatgtaagg caagcaggag gggtctgaag acgcgaaaaa agcttgagct
     1141 tttcttctca tgtgagacgc gcaggcagcg agagcggagt gaaaccctgt ccttgagtcg
     1201 ctcaatcagt acacgtgaaa catcgaagga ggcatcacta tcgagaccct tattctgcac
     1261 accactcgcg gtatcctcaa cccgaagacg ctgaaggaag cgcaagcaat gcacaacgct
     1321 ttcgtgatgg agggcccgca gcccgggatc gcaatcgctc gttcgctcgg agatcttagc
     1381 cacaacctgt atacgcccgt ggagggtctg gggaatctgt cggcagcttc gccaggcgaa
     1441 ctgctcttca tcgattattg ggccgatcca ggcggtatgg agacattttt ctccaatccg
     1501 ttcgtacagg aggcggcgga ccacctgtat tcatcgcggg aggaagcgga gtggataccc
     1561 gcgcgcgatg cgttcacgtt ccatgtccca gcggtagttg gcacgactgc gcgtttcgtc
     1621 gggatgatgc gggcaccggt gcgtgcgtcc tcggatgcgg tcacggtcct ctcggaactc
     1681 atctgggcaa acctccgtgc atcgcgcaga ctcggccatc tctcgcatca gctcttcctg
     1741 cggcatgctg ccgtcgtgga agccaggcca gcagcgaatg cacgccgcag cggtggtgaa
     1801 gacaccgcct tgccggtcga gccagtcgaa gtccttgccg tcgatttctg gccaacgctt
     1861 gaagggctca ccgagcacta ccgtgccttg ctatccacga acgggctcga cgaggtgttg
     1921 gccgggtccc tcacagtgtc cgtctgggag gcggcgagcg gattcgtcgc gtggtaacac
     1981 gcacgaaagg aaaccgtctc ggctggcacg
//
LOCUS       DASZAK010001229         6462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_280928, whole genome shotgun sequence.
ACCESSION   DASZAK010001229 DASZAK010000000
VERSION     DASZAK010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280928"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VGT44_21600"
     CDS             complement(<1..497)
                     /locus_tag="VGT44_21600"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2583470.1"
                     /translation="MTVLNSRNDLDTIPVLSDDVPVTMPELPVRFTITSFEQFKAISE
                     PMRSRILGIIQNQPATAKQIAERLGATPGAIGHHLHVLEDAGLAKIMARRLVRGIVAN
                     YYTRTARIFNYNLPEEVSGSMDASLEIMNKAHNEMMEAHSTIKDDPYRRDGFPHIRLS
                     QERAQ"
     gene            800..1264
                     /locus_tag="VGT44_21605"
     CDS             800..1264
                     /locus_tag="VGT44_21605"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV2583471.1"
                     /translation="MKEKIKGILAGLIYRIIVILTLGQISPLLGAGIIIEQEGKILLI
                     DRADGLGYTVPGGIVRHKETVEQCVLRETREETGYSVQITGLVGVYSSPNRDPRFRAV
                     AIAYTGKIIDGTPRGSGEGQPCWRTPDEVLGHMAFDCEQMLKDYLNGKINLS"
     gene            1406..2446
                     /locus_tag="VGT44_21610"
     CDS             1406..2446
                     /locus_tag="VGT44_21610"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV2583472.1"
                     /translation="MPKQPGKRNKSVAVAIEIAGSAAKIALVDRSGSIQRRVDARTLW
                     GRSSLATLEPYLRSIEDMLAYAKGQGWRVRGIGVALPGILDAEARRPLHLPVLPSLNN
                     FPLCDLLEARYKLPAHLVVDVDAALIGEWYHGAGQGYERLLYITLNAVVGASSIIDGQ
                     LVRGAGGSQSAVGHVAHVTISAAGQRCGCGKRGCINSFVSQEALQKMVLRALRRDEET
                     SLSQRLAQREPLSVQMLVEEAQRGDSVAKYIYYDIERWLSEAIARALRLFEPDILILG
                     GAMPTTSPTFLARIREAGMTADYGFHIAKTELGSDGTLIGVTTPFFTRRPDPVTESHI
                     PALYKVQADTPL"
     gene            2625..3482
                     /locus_tag="VGT44_21615"
     CDS             2625..3482
                     /locus_tag="VGT44_21615"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV2583473.1"
                     /translation="MSPLAIGLVLVAAVLHAIWNLLVKRAREKQVMTWLALVTGVVIY
                     LPIVIVQPIDVWRVWPFIICSALVEAIYYIALISAYQHGDFSLVYPMARGTAPALLLI
                     WTTIFLGERPKLTGVLGILLLVLGLVIVGGKAWWSLRKTASLASNGLAIALGVAMCIS
                     VYTTIDGAAVQRVNPLPYTVIVIALTTAIITPAIVLRYGKVKIIEEWRSNWPRIMLVG
                     AFTLLAYILVLKAYTLTRVSYAGSVREVSVVFAALLGWKLLREEFGAARVIGSICIFS
                     GILVIALAG"
     gene            3510..4274
                     /locus_tag="VGT44_21620"
     CDS             3510..4274
                     /locus_tag="VGT44_21620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583474.1"
                     /translation="MHDTTIPVIVIDDTPVKLRGICEFVEETPQLRLAARAGDMNAAI
                     VYVDQFCKEQAHTNPTLEGWMILSDLHLGTDNGVRLGRLLLERAAGLRVVIYTQDPSW
                     TMAAEIFRQEYTRGAQRGASKGAKKPPGLHGYALFGNIEPAYLERIAAAVVLRRETFI
                     DPEVLNYLLKRLRDQRLTPRQEECAMLIARGLSNDDIALRMGFFNAQGHPNMGPLENI
                     VSELYSFFAIEGAPSDPGRRVLLAHAYEAYAGLRPA"
     gene            complement(4277..6235)
                     /locus_tag="VGT44_21625"
     CDS             complement(4277..6235)
                     /locus_tag="VGT44_21625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583475.1"
                     /translation="MKDRTTQRSWRQLRRRLTFLYLVVGFTLLLISGIGHARLLSEVG
                     KTFGGFFWAIDTDKQVVVVSTPPQLATFSADPGSLTNTTVLVGVDGKPISALISVYQH
                     TTPGQTIHYKVEQNGQLRTIDRAASIFTWDMWFSNYGWALIAGICWLLVGGILLATSQ
                     DWTGAVEGITLLPPAMLFLLYSHWGNVQQAYPADLVFQLLWVPAFALLGAGFIHLSLT
                     YRPQALRTPRNPSRWIDGAPYLPLVALLAFDVSTLLIRGSVPTRIEFILALGYAVLGG
                     LVSLAVGLTSLMRVLRILPGTAAVPVPVRRRLGDLLTLWIGGVGLGFGLGILPILLTG
                     RPLVPLLVFYVLAAAYPLVLLYAIRSLRLIARLEVILEQREDALREQQKTAEQLRQSN
                     RKLEEATSLLLYADAHQRSILSQRIHDQPKQHALRIRSLLGHWQHKLKLETENDAHLR
                     AFAQPVIEALGKVRKISEELESDLRGLQLLVEDAYRRRNLGLKLHLETLIREDLPALH
                     PESQLKIRPDLWALDDLSHDLEQETEGEKFAEAISYTVTQALLNIYNHAGATFATVRA
                     ARTNGHIEIMITDDGHGFDTNAIPPEKTSLFKARLKAEEAGGALTMFSAPRSQAQHGT
                     TILLHLPLPPSQLATHSGPLAESVMEEL"
     gene            6261..>6462
                     /locus_tag="VGT44_21630"
     CDS             6261..>6462
                     /locus_tag="VGT44_21630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583476.1"
                     /translation="MQNRHAQGKNTEPSPWHLAIARGSLAIALAQVYAKPGSLPHLKH
                     IAVVNRQNLVRPHYFAAIVRDRA"
BASE COUNT         1330 a         1841 c         1914 g         1377 t
ORIGIN      
        1 tactgggcgc gttcctgtga aaggcgaatg tgtggaaaac catcgcggcg atacggatcg
       61 tctttgatgg tggaatgagc ttccatcatt tcattatgtg ctttgttcat gatctcaagc
      121 gaagcatcca tgctacctga cacttcctct ggcaggttgt agttgaagat acgcgctgtg
      181 cgtgtgtagt aattggcaac gattccgcgc accagccgcc gggccataat cttagccagt
      241 cccgcatctt ccagcacatg caaatgatgc ccaattgcac ctggcgttgc cccaagtctc
      301 tccgctatct gcttggccgt agcgggctga ttctgaatta tgccaaggat acgcgagcgc
      361 atcggctcgc taatagcctt gaactgctcg aaagaggtga tagtgaaccg cacaggcaac
      421 tcaggcatgg ttacaggtac atcatccgac agcaccggga tagtatcgag atcgtttctg
      481 ctattcaata cagtcacaaa atgttccttt ctcgcatgtg caagaaaaat ggtatgtgct
      541 aatatttcag cacatgatga aagtatagta cgaaaaataa ggaatgtcaa tggacgggtg
      601 caggatccta cacccatgat gcgatgatgc gtctatgcct caaaaccttg ccatctatcg
      661 cattcattca tatacttgca agtatcattg aggcaattcg cgcattggcc gaacttatag
      721 gcatcttctt gctacaatat gtgagggaga caggtcgcga aaatttgaag cggatacctg
      781 gaagggaaca gcgtcgagca tgaaagagaa aatcaagggc atcctggcgg gacttattta
      841 tcgcatcatc gtaatactca ccctcggaca aatttccccg ctgttgggcg cgggcatcat
      901 catagagcag gagggcaaaa tcctgctgat cgatcgcgcc gacgggctgg gctataccgt
      961 tcccggcggc attgtgcgcc acaaggaaac ggtggaacag tgcgtcctgc gcgagacacg
     1021 cgaagagact ggctacagcg tccagattac cgggctggtc ggcgtctatt catcacctaa
     1081 tcgcgaccca cgtttccgag ccgtcgccat cgcctatact ggcaaaatca tcgatggcac
     1141 cccgcgcggc tccggcgaag gccaaccctg ctggcgcaca cccgatgagg tgctgggcca
     1201 catggccttc gattgcgagc agatgctcaa agactacctg aacggcaaaa tcaacctctc
     1261 ttgaacatct gctcttgaca aatgttctta cggctcatta tactttgatt acatcaaatg
     1321 gtgtaatagt acctgtaatt gttgctgtag ccctgtgaat aatagaacgc acgttctata
     1381 tatttgctag aatcttgagt tccaggtgcc gaaacagccg gggaagagaa acaagagcgt
     1441 cgcggtcgcc atagagatag cgggcagcgc ggctaagatt gcgctggttg atcgttccgg
     1501 cagtatccag cgcagggtcg atgcccgcac cctctgggga agatcatcgc tggccacgct
     1561 tgagccatac ttgcgctcca ttgaagacat gctggcctac gcaaagggcc agggctggcg
     1621 cgtgcgcggc atcggcgtcg cgctgcccgg catccttgat gctgaggcgc gcaggccact
     1681 gcacttgcct gtgctgccct cgctcaacaa tttcccgctt tgtgacctgc tggaggcgcg
     1741 ctataagctt cccgcccacc tcgtcgtcga cgtggatgcc gcgctgattg gagaatggta
     1801 tcacggcgca ggccagggct acgagcgcct gttgtatatc acgctcaacg ccgtggtagg
     1861 ggcgtcatcg atcatcgatg ggcagctcgt aagaggcgcg ggcggctccc aatctgctgt
     1921 cgggcatgtt gcgcacgtga cgattagcgc ggctggtcag cgctgcggct gtggcaaacg
     1981 cggctgcatc aactcgtttg tgtcgcaaga agccttgcaa aaaatggtac tgcgcgccct
     2041 gcggcgtgac gaggagacca gcctctcgca gcgacttgcc caacgcgaac cgctcagcgt
     2101 gcaaatgctg gtagaagagg cgcagcgcgg cgactcagtg gcaaagtaca tatattacga
     2161 catagaacgc tggctgagcg aggccatcgc gcgcgcgctg cgcctgtttg agccggatat
     2221 cctcattctg ggcggcgcga tgcccacaac cagcccgacc tttctcgccc gcatccgcga
     2281 agccggcatg accgccgatt atggctttca tatagccaaa acagaactcg gcagcgatgg
     2341 cacgctaatc ggtgtcacca cgcccttttt cacccgacgt cccgaccccg tgaccgaatc
     2401 gcacatcccc gcgctgtata aagtacaggc tgatacgcct ctgtaggggc gggggttgat
     2461 gtggtgtggg gaggggacgc gtgcgtcgcc cgcgtacctg gtggcctatg tttcccttgc
     2521 atcacgttgt cgatgcatga accggctacg cgggcgacgc aagcgccccc tcccttcccc
     2581 cgttcctacg gacagatata cggacacacg aaggagaata gtcaatgtcc ccgcttgcta
     2641 tcgggttggt gttggtagcg gcagtattac atgccatatg gaatctgctg gtgaaacgcg
     2701 ccagggagaa gcaagttatg acctggctgg cgctggtgac cggggttgtc atctatttgc
     2761 ccatcgtgat agttcagccc attgatgtgt ggcgtgtctg gccgttcatt atttgcagcg
     2821 cgctggttga ggcgatctac tatatagcct tgatctccgc ctatcaacac ggcgattttt
     2881 cgctggtcta cccgatggcg cgtggcacag ctcctgcgtt gcttttgatc tggacgacga
     2941 tctttcttgg cgagcgtcca aaactcaccg gggttctggg catcttgttg cttgtgcttg
     3001 gcctcgtgat tgttggggga aaagcctggt ggtcgctgcg gaaaaccgcg tcgttagcct
     3061 cgaacggcct cgcgatagcg ctgggtgtcg ccatgtgcat ctccgtctac accaccatcg
     3121 atggcgcggc tgtccagcgc gttaatccct tgccatatac cgttattgtc attgcgctca
     3181 cgaccgccat catcacaccc gctattgtgc tgcgctatgg caaagtgaag atcatcgagg
     3241 agtggcggtc gaactggccg cgcatcatgc tcgtgggcgc ttttacacta ctcgcctata
     3301 tcctggtgct gaaagcctat acgctgacgc gcgtcagtta tgccgggtcc gtgcgcgagg
     3361 tcagcgttgt ctttgccgct ctgctaggtt ggaaactgct gcgcgaggaa tttggcgcgg
     3421 cgcgcgtgat cggctcgatc tgcatcttct ccggcatcct ggtgatcgcg ctcgcgggtt
     3481 aagccggttg tgataggata taaagtgcta tgcatgatac aacgatcccg gtcatcgtca
     3541 tcgatgatac gcctgtgaaa ctgcgcggca tctgcgagtt cgtcgaggag acgccgcagt
     3601 tgcgcctcgc cgccagggcg ggcgatatga acgctgctat tgtctatgtc gatcagttct
     3661 gcaaggaaca ggcacatacc aatccaacgt tggagggttg gatgatcctc tccgacctgc
     3721 atcttggcac ggacaatggt gtgcgcctgg ggcgactcct gttagaacgc gctgcgggat
     3781 tgcgcgtggt gatctacacc caggacccca gttggacgat ggcggcggag atcttccgcc
     3841 aggaatatac gcgaggagcg cagcgcggcg cgagcaaggg cgcgaaaaag ccgccagggt
     3901 tgcacggtta cgcgctgttc ggcaacatag aaccggcgta tttagagcgc atcgccgccg
     3961 ccgttgtgtt gcgccgcgaa acctttatcg accccgaagt gctgaattat ctactcaagc
     4021 gcctgcgcga tcagcgcctg acaccgcgcc aggaggaatg cgcaatgctg atcgcgcggg
     4081 ggctgagcaa cgacgatatt gcactgcgca tgggcttctt taatgcgcag ggacatccta
     4141 acatggggcc gttggagaat atcgtcagcg aactctacag cttctttgcc atcgaaggcg
     4201 cgccgagcga tcccggcagg cgcgtcctgc tggcccatgc ctacgaagcc tatgccggtc
     4261 tgcgaccggc gtagcattac aattcctcca tcactgactc cgcaagtgga ccgctgtgcg
     4321 tcgccaattg tgagggcggt aggggcaggt gcagcaggat cgtggtgcca tgttgggcct
     4381 ggctgcgcgg cgcggaaaac atggtaagcg cgccgcccgc ctcctctgcc ttgagtctgg
     4441 ccttgaacag gctggttttt tcgggcggga tcgcgtttgt atcgaagccg tgaccatcgt
     4501 cggtaatcat gatctctatg tgtccgttcg tgcgcgctgc ccgcacggtg gcgaaggtcg
     4561 cgccggcgtg gttgtagata ttcagcagcg cctgtgtcac ggtgtaagaa atggcctccg
     4621 cgaacttttc cccttcggtt tcttgctcca ggtcatggct cagatcatcg agtgcccaca
     4681 ggtcggggcg aatcttgagc tgggattccg ggtgcagcgc aggcaggtct tcgcggatga
     4741 gcgtctccag gtggagcttc agccccaggt tgcgccgccg gtaggcatcc tcgaccagca
     4801 gttgcagccc gcgcaggtcg ctttcgagtt cttcgctgat cttgcgcacc ttgcccagcg
     4861 cctcaatcac cggctgcgca aaggcgcgca ggtgggcgtc gttctccgtt tccagcttga
     4921 gtttgtgctg ccagtggccg agcagagaac ggatgcgcag cgcgtgctgt ttgggctggt
     4981 cgtggatgcg ctgcgaaagg atggatcgct ggtgcgcatc ggcgtacaac agcagcgagg
     5041 tcgcttcttc cagcttgcga ttcgactggc gcagctgctc cgccgtcttc tgctgctcgc
     5101 gcagagcgtc ttcgcgctgc tccaggatga cttccaggcg ggcgatcagg cgcagggagc
     5161 ggatagcgta gagcagcacc agtgggtagg cggctgccag cacgtagaag accagcagcg
     5221 gaacgagcgg tctgcccgtc agtagaattg gtaggatgcc aaggccaaaa cccaggccga
     5281 ctccgccgat ccacagcgtc aacaggtcgc ccaggcggcg gcgtaccggc actggcacag
     5341 cggcagtgcc cggcaagatg cgcaagacac gcatcaaaga cgtgaggccg actgccaggc
     5401 tgaccagccc gcccagcacg gcgtacccca gcgccaggat gaactcgatg cgcgtcggta
     5461 cgctgccccg gatcagcagc gtactcacgt cgaatgccag cagcgccacc aggggcagat
     5521 agggcgctcc gtcgatccag cggcttggat tgcgcggggt tctcaatgct tgcgggcggt
     5581 aggtgaggct gagatggata aatccagcgc ccagcagcgc aaaggccggg acccacagca
     5641 gttgaaagac caggtccgcc gggtaagcct gctggacgtt gccccagtgg ctgtagagca
     5701 ggaagagcat cgccgggggc aatagcgtta tgccttcgac cgcgcccgtc caatcctgcg
     5761 aagtggccag cagtataccg ccaaccagca gccagcaaat gcccgcgatc aaggcccagc
     5821 cgtagttgct gaaccacata tcccaggtaa agatactcgc tgcacgatca atcgtcctca
     5881 actgcccgtt ttgctcaact ttatagtgaa tggtctggcc cggcgtcgtg tgttgataga
     5941 ccgagatgag cgcggaaatg ggtttgccgt caacgccgac cagcacggtt gtattcgtca
     6001 gcgaacccgg atcagccgag aaggtagcaa gttgcggcgg cgtcgagaca acaacgacct
     6061 gtttatctgt atcgatggcc cagaaaaacc ctccgaaggt tttgccgact tcgctcaaga
     6121 gacgcgcatg tccgatgccg ctgatgagga gcagcgtgaa tccaacgacc agatagagga
     6181 atgtcaggcg tcggcggagt tgcctccacg aacgttgtgt tgtgcgatct ttcatcaaaa
     6241 tagacacact ctagtgtaac atgcagaaca ggcacgcgca aggcaaaaat actgagccat
     6301 cgccctggca cctggccatc gcccgggggt cacttgccat cgccctggct caagtgtatg
     6361 cgaaaccagg ttcgcttccc cacctgaaac acatcgccgt tgtgaatcgt caaaacctgg
     6421 tccgaccgca ctattttgct gccattgtgc gtgatcgcgc ct
//
LOCUS       DASZAK010001230         1937 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_387560, whole genome shotgun sequence.
ACCESSION   DASZAK010001230 DASZAK010000000
VERSION     DASZAK010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387560"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VGT44_21635"
     CDS             complement(<1..300)
                     /locus_tag="VGT44_21635"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEV2583477.1"
                     /translation="MSELIVETRYGKVLAYEQGSISIWKGIPFAQPPTGERRFRAPQP
                     PQPWTGVREATAFSPMAPQAPEVGASMVGAVGAERAVEPRPMSEDCLYLNIWSPGA"
     gene            445..846
                     /locus_tag="VGT44_21640"
     CDS             445..846
                     /locus_tag="VGT44_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583478.1"
                     /translation="MRAIMSDGLRFLERVEPTESPHAALRATFEELASLYQQQRTSFL
                     LHYQVAMQIPSVVPIYLAVLIETELALCDMLYSCLETGTNRNQVRFLVALYMTALRVA
                     LEEWLEQEAQGDLVSLLRGYLDSFSSLSHQA"
     gene            complement(869..>1937)
                     /locus_tag="VGT44_21645"
     CDS             complement(869..>1937)
                     /locus_tag="VGT44_21645"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEV2583479.1"
                     /translation="YPMQSYYGRGDPGGRPGRRTIMPDLTTTYLGLALKNPLVASASP
                     LSRKLDTARRLEDAGASAIVMYSLFEEQITHESHELDHFLERGTHSYAESLSYFPDLD
                     HYNLEPEQYLEHLQRVKQAVAIPVIGSLNGISSGGWVEYARKIEQAGADALELNIYYV
                     PTDVDLSGAELEENYVQLVRDVRARVTLPIALKLSPFFTSLPHMARSFVGAGANGLVL
                     FNRFYQPDFDLEALEIVPNLVLSTPNDLRLPLRWVAILYGRVAADLALTSGVHTAQDV
                     LKAMMAGANVAMMASTLLHNGIGRIMHILNDLQEWMEVHEYESITQMRGSMSQRAVAD
                     PAALERANYMKELNSFNSYVR"
BASE COUNT          369 a          590 c          537 g          441 t
ORIGIN      
        1 tgcgcctggc gaccagatgt tgaggtacag acaatcctcg ctcatcgggc gcggctctac
       61 ggctctctct gctcctaccg ctcctaccat gctcgcgccg acctccggcg cctggggcgc
      121 catggggcta aacgcggtcg cttcgcgcac acccgtccag ggctggggcg gctgcggtgc
      181 ccgaaaccga cgctctccgg tggggggctg cgcgaaaggt atgcctttcc agatgctgat
      241 cgagccttgc tcgtaggcca gaactttgcc ataccgcgtc tcaacgatca attcactcat
      301 ctatttgctc ctgcagcttt ttctctttct ctggtgagaa acgactacgc taaatccagg
      361 acgactgctt ctttcctctg cgctcaagaa gtgagctcct tttttcgtta ctttgcgtcc
      421 aaagaggagg tgctctttgc gccgatgcgc gcgatcatga gcgatggtct gcgctttctt
      481 gagcgcgtgg agccaacgga gtcaccgcat gcagccctgc gcgcgacttt tgaggagctg
      541 gccagcctct atcagcagca gcggaccagc tttctgctcc actatcaggt cgccatgcag
      601 atcccatcgg tcgttcccat ctatcttgct gtcctgatag aaacagagct agctctctgt
      661 gatatgctgt actcttgcct ggaaacggga acgaatcgca accaggtgcg cttcctggtg
      721 gccctctaca tgaccgcgct acgggtggca cttgaagagt ggcttgagca agaagcgcag
      781 ggagatctgg tttccttgct tcgtgggtac ctggactcct tctcctcgct ttcccaccag
      841 gcctagacgc gcacttacca tcggtgtctt acctcacgta tgaattaaac gaattaagct
      901 ccttcatata attggcgcgt tcgagggccg cggggtcggc gacggcgcgc tggctcatac
      961 tgccccgcat ctgcgtaatc gactcgtatt cgtgtacttc catccactcc tggaggtcat
     1021 tcaagatgtg catgatccgc ccaatcccgt tgtgcaggag cgttgaggcc atcatcgcga
     1081 catttgctcc ggccatcatg gccttcaaga catcctgtgc ggtgtgtact ccgctcgtca
     1141 gcgcgagatc ggcggcgacg cgcccataca gaatggcaac ccagcgcagg ggcaggcgca
     1201 aatcgttcgg cgtactcaga accaggtttg gcacgatctc caatgcttcc aggtcaaagt
     1261 ccggctggta gaagcgattg aacagcacca gtccattcgc gcctgctccg acaaaacttc
     1321 tcgccatatg tggcagcgat gtgaaaaacg ggctgagttt gagcgcgatg ggcagcgtca
     1381 ccctggcgcg cacgtcgcgc accagctgga cgtagttctc ttccagttcc gcgccgctca
     1441 ggtcaacgtc cgtcggcaca tagtaaatgt tcaactcaag cgcgtcagcg cccgcctgtt
     1501 cgatcttacg cgcgtactcg acccagccgc cggacgagat gccgttcaga ctgccgatca
     1561 ccgggattgc aaccgcctgt ttgacgcgct gcagatgctc aagatactgt tccggctcca
     1621 ggttgtagtg atccaggtcg ggaaagtagc tgagcgattc ggcgtaagaa tgcgtgccac
     1681 gttccaggaa gtgatccagc tcgtgacttt catgcgtgat ttgttcttca aacagcgaat
     1741 acatcacgat cgctgaggcg ccggcgtctt ccaggcggcg cgccgtatcc agcttgcgcg
     1801 agagcggcga cgctgaagcc accaacggat ttttcagtgc taaccccagg taggttgtcg
     1861 tgagatcggg catgatagtt ctccttccag ggcgaccgcc agggtcgccc ctaccatagt
     1921 acgattgcat cggatac
//
LOCUS       DASZAK010001231         5674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_422505, whole genome shotgun sequence.
ACCESSION   DASZAK010001231 DASZAK010000000
VERSION     DASZAK010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422505"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..887)
                     /locus_tag="VGT44_21650"
     CDS             complement(<1..887)
                     /locus_tag="VGT44_21650"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEV2583480.1"
                     /translation="MRFSLQDIPKRVQRRGGELTVTLHFLRRGEARDEIARLIAYHEQ
                     MLGQPQRAFSIDDARTLVGDYRLAHCLLAALSAWYSWRSPGWDDVIGRMGGDAGIGAL
                     AETGITSPVQLRLALFDYVNARHAGFLDAHNRLSALEQFAASYGLAVADLEYLLLVDT
                     DEEAVLVRDGSERPPSPDEVAALYNQWVFEAAMFNASEAHFVIDCAAFLAASKEHTAS
                     STPAGIGSVVKRLCFFARKLGVYYDLVYEGANLLHLTLYGPQDMTGAPQQYGARLARL
                     CRLLLGYGAPARSSKPTPTA"
     gene            complement(900..2306)
                     /locus_tag="VGT44_21655"
     CDS             complement(900..2306)
                     /locus_tag="VGT44_21655"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV2583481.1"
                     /translation="MTTLDHRGRDLAIIPDESLLHLPTEALRSALFGSHLPASVAASM
                     ERDKRQHFLRTQPMHFAAVKRALQAQGVPFHIAFEERPALPFETGLGIEARPYQQEAL
                     AHWLAAGSAGVVILPTGAGKTFVAAMALHETRLWTLVVVPTLDLLRQWRVALAGALSL
                     DAEEIGIFGGGEKEIKPITIITYDSAALYPRELRRFGLLVFDECHHLPAPGYRLIAES
                     AFTPLRLGLSATPERGDMAHLDLDYLIGPEVYRRSPAELTEERYLAQYEEVRIDIALS
                     SEDEARYNEQRRIYRAFLQRRHIRITSPEEFQQKVIFMSARDAQAREAMLAWREARNI
                     AMNAPAKYEEIERLLHQHAGDQVIIFSEYNQVVDEIRRRSCLPSITYKTPAEERRIVL
                     DRFRSGQYTKLVTGRVLNEGVDVPDCRVAIIVSGNSTKREYIQRLGRVLRPKTGQAVL
                     YELVTSGTTEEDIARRRK"
     gene            complement(2293..3309)
                     /locus_tag="VGT44_21660"
     CDS             complement(2293..3309)
                     /locus_tag="VGT44_21660"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEV2583482.1"
                     /translation="MERDLQEQACWLLLVFESGLSTRVANNLLVEWCHQRKRSLREFF
                     AADAAEWKEVCQLNDDAIKKLEASKEKLVGQAFLAEQLAHNQVHLLTVLDDDYPKLLK
                     VSLKREQTPTVLFYAGDLGILERKAIAIIGSRNASEESLAFTRAAARYCAEQGANVIS
                     GNARGVDRAAYEGATSGDGRTTVVLPHGMRKLSGAQMRALTPQIEAGQALLISQFHPD
                     AQWVVSRAMERNKVVTGLAQIVIVAQSDVQGGTWEGANGALKQHRPLYVRQPGAGTPL
                     PGNQALIERGGHPLAWPLADEAALATELHPLVEESAVLQQQKEEQAPRPDQLSLFAGT
                     NDNA"
     gene            complement(3401..5446)
                     /locus_tag="VGT44_21665"
     CDS             complement(3401..5446)
                     /locus_tag="VGT44_21665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEV2583483.1"
                     /translation="MTTAVTWQSNTPEQLLKERFHISTGFHAGQREIIEQLVQGKRLL
                     VIQRTGWGKSLCYQMASLYYPHLTLVFSPLKALMRDQCQRCNDVYGIPAGIVSSEFEP
                     EENRAMLERAIEGQLKILFIAPERLDNSDWQRSITQMRVSMIVIDEAHCISTWGHDFR
                     PHYQRIVRLLSALPGNVPVLALTATANKRVEADVLAQIGAGAQVVRGTMQRPHLYLNV
                     VELRGEREKLAYLAELLPRLPGTGIIYTATQHDAEMVAAFLQGQGVEAEYYHAKRDED
                     VRLAIEQKLMANGYKVVCSTNALGMGIDKPDIRFVIHFQVPGSPIHYYQEIGRAGRDG
                     KVAWCLLLFDQNDLRVQEYFIQSAKPAAQCYEFVLSLLQIKPCGQKEIMQLTGYAQKT
                     VQQILFDLEELHVIERDAKARTYSAIQQLAKIEFSAYDAVREQKQRELQDMQDYARLR
                     TCYMEYLTTYLGDEPGHGCRCCGNCRASNFPVVRYSTRIYQTAGHFLEVEYLPHIEKR
                     GGERRPEHEAGWSLSYHGGTRTGKLVRASKYEEAGPFASDLVRRAVEVIRARYPIDAI
                     DGIVSVPPTKSGALVETFARQVAVQLGIAYMPVVEKVRETDEQKSFTNWVQKQKNVKG
                     AFSVPEPEHMAGCALLLIDDIYDSGYMLREVGLTLMRAGAKAVYPFTITRTAHSDDQ"
BASE COUNT         1117 a         1816 c         1600 g         1141 t
ORIGIN      
        1 gcggtcggcg ttggtttcga cgaccgcgca ggcgcaccgt accctagcag caaacggcac
       61 agccgcgcca accgcgcacc atactgctga ggcgcgccgg tcatatcctg cggaccatag
      121 agcgtaaggt gcaataaatt cgcgccctcg tataccaggt cataatacac acccagcttg
      181 cgcgcgaaga aacagaggcg tttcacaacc gacccgatac ccgccggggt agaagaggca
      241 gtatgctcct tactggcagc cagaaaagcc gcgcaatcaa tcacaaaatg cgcctcggac
      301 gcattgaaca tggcggcctc aaacacccac tgattgtaga gcgccgctac ttcatccggc
      361 gagggaggcc gctcgcttcc gtctcgaact aacacggctt cctcatcggt atcaaccagc
      421 agcaggtatt ccaggtcggc gacggccaga ccatacgacg cggcgaattg ctccagggcg
      481 gacaaacgat tgtgcgcgtc cagaaagcca gcgtgacgcg cattgacgta gtcgaacaga
      541 gccagcctga gctgcacagg cgatgtaatg cccgtctcgg ccagcgcgcc aatgcctgca
      601 tcgccgccca tgcgccctat cacatcatcc cagccgggcg agcgccagct ataccacgcg
      661 ctaagcgcag ccagcaggca gtgcgccagg cgataatcgc ccaccagggt gcgcgcatcg
      721 tcgatgctaa aggcgcgctg cggctggccc aacatctgct cgtgataggc gatcagccgc
      781 gcgatctcgt cacgcgcttc gcccctgcgc agaaaatgca gggtgacggt cagttcgcct
      841 ccgcgccgct gcacgcgctt cggaatgtct tgcaggctaa aacgcactca ttgctccttt
      901 tatttccggc gtctcgctat atcttcctcg gtcgttccgc tcgtgaccag ttcatacagc
      961 accgcttgcc cggtcttggg gcgcaggacg cggcccaggc gctggatata ttcgcgcttc
     1021 gtgctgttgc cgctgacgat gatggccacg cggcaatccg gcacatccac gccctcgttg
     1081 agaacgcggc ccgtcaccag tttggtatac tggccggagc ggaagcggtc gaggacgatg
     1141 cggcgttcct cggcgggtgt cttgtaggta atactgggca ggcacgaacg gcggcgaatc
     1201 tcatcaacaa cctggttata ctccgagaag atgataacct ggtcgcccgc atgttggtgc
     1261 agcaagcgtt cgatctcttc atatttggcg ggcgcgttca tggcgatgtt gcgcgcctcg
     1321 cgccatgcca gcattgcctc gcgcgcctgg gcatcgcggg cgctcatgaa gataactttc
     1381 tgctgaaact cttcgggcga ggtgatgcga atatgccgcc gctgcaagaa ggcgcgatag
     1441 atgcgccgct gctcattgta gcgcgcctcg tcctcgctcg acagggcaat atcgatgcgt
     1501 acctcctcgt actgcgccag gtagcgttct tctgtcaatt cggcgggcga gcggcggtag
     1561 acctctggcc cgatcaggta atccaggtcg agatgcgcca tgtcgccgcg ttccggcgtg
     1621 gcgctcagcc ccaggcgcag tggtgtgaag gcgctttcgg cgatgagcct gtagccgggc
     1681 gcgggcaggt ggtgacattc atcgaagacc agcagcccga agcggcgcag ttcgcgcggg
     1741 taaagcgcgg cggaatcata ggtgataatc gtgatgggct tgatctcttt ttcgccgccg
     1801 ccaaaaatgc cgatctcctc cgcgtcgagc gacagcgcgc ccgccagcgc cacacgccac
     1861 tgtcgcagca gatcaagcgt cggcacgacc accagcgtcc acaagcgcgt ctcatgcagc
     1921 gccatcgccg cgacaaaggt cttgcctgcg cccgtcggca agataaccac gcccgcgctg
     1981 cccgccgcca gccagtgcgc caatgcctcc tgctggtacg gtcgggcttc aatgcccagc
     2041 cccgtctcaa acggcagcgc aggccgttcc tcaaaggcga tatgaaatgg cactccctgc
     2101 gcctgcaaag cgcgtttcac tgcggcaaaa tgcatcggct gtgtgcgcag gaaatgctgg
     2161 cgtttgtcgc gctccatact cgcggcaacg gatgcgggaa ggtgcgagcc aaacagggcg
     2221 ctccgcaacg cctccgtcgg taaatgcagc aaggattcgt caggaatgat ggcgaggtcg
     2281 cggccccgat gatcaagcgt tgtcatttgt ccccgcaaac aaactcaact gatccgggcg
     2341 cggcgcctgc tcctccttct gctgctgtaa gaccgcgctt tcctctacga gcgggtgaag
     2401 ttctgtcgcg agggctgcct cgtctgccag tggccaggcc aggggatggc cgccgcgctc
     2461 gatcaacgcc tggttgcccg gcaggggagt accggcgccg ggctggcgca cgtagagcgg
     2521 gcgatgctgt ttgagcgcgc cgttcgcgcc ttcccaggtg ccgccctgca catccgattg
     2581 cgccacaatc acgatctgcg ccaggccggt gacgaccttg ttgcgctcca tagcgcggct
     2641 gacgacccac tgcgcgtcag ggtgaaactg gctgataagc agcgcctggc ccgcctcgat
     2701 ctgcggcgtc agcgctcgca tctgcgcccc gctcagcttg cgcataccat gcggcagcac
     2761 gacggtcgtg cggccatcgc cgctggtcgc gccctcgtat gcggcgcgat caaccccgcg
     2821 cgcgttaccg ctgatgacgt tcgcgccctg ttccgcgcaa tagcgagcgg cggcgcgtgt
     2881 gaaagccagg ctctcctcgc tggcattgcg cgagccgatg atagcgatgg ccttgcgttc
     2941 caggatgccc aggtctccgg catagaacag cacagtcggt gtctgctcgc gcttgagcga
     3001 gaccttgagc agttttggat aatcatcatc gaggacggtc agcagatgta cctggttgtg
     3061 cgcgagctgt tcggccagaa acgcctgccc gacgagcttc tctttgctgg cctccagctt
     3121 cttgatcgca tcgtcattga gctggcagac ctccttccac tccgcggcat cggcggcgaa
     3181 gaactcgcgc agcgagcgtt tgcgctggtg gcaccattcc acgagcaggt tattggccac
     3241 gcgcgtcgat aatccgctct cgaaaacgag caacagccag cacgcctgct cctgtaaatc
     3301 gcgttccatc tcaatgtatc ctttactaaa agcgtgattt ggtggagcat ggcgaccgca
     3361 agggtcgcca tgctcctaca cgccatactc acacatgcat ctactgatcg tccgaatggg
     3421 ctgtgcgcgt aatcgtaaac ggataaaccg ccttcgcgcc cgcccgcatg agcgttaacc
     3481 caacctcgcg cagcatatag cccgaatcgt aaatatcgtc gatgagcagc agcgcgcagc
     3541 ccgccatatg ttcgggttcc ggtacgctga aggcaccttt gacgttcttc tgtttctgca
     3601 cccaattcgt gaaacttttc tgctcatctg tctcgcgcac cttctcgaca accggcatgt
     3661 aggcgatgcc gagctgtacc gccacctgcc gggcgaaggt ttccaccagc gcgccgcttt
     3721 tcgtgggcgg cacgcttact ataccatcga tcgcgtcgat tggatagcgc gcgcgaatga
     3781 cctcgacagc gcgccgcaca aggtcggacg caaacgggcc tgcttcttca tacttgctgg
     3841 cccgtactaa tttcccggtg cgcgttccgc cgtgatacga tagcgaccag cccgcctcgt
     3901 gctccgggcg acgctcgccg ccgcgtttct cgatatgcgg caggtactcc acttcaagga
     3961 aatgccctgc tgtctgatat attcttgttg aatagcgcac aaccggaaaa ttcgacgcgc
     4021 ggcaattccc acaacaccgg catccgtgtc ccggctcatc gcccaggtac gtcgtcagat
     4081 attccatata acaggtcctg agccgcgcat agtcctgcat atcctgcaac tcgcgctgtt
     4141 tctgctcgcg cacggcatca tatgcgctaa attcgatctt cgccagttgt tgaattgccg
     4201 agtacgtgcg cgccttcgcg tcgcgttcga tcacatgcaa ctcttcgaga tcgaacagaa
     4261 tttgctgcac ggttttctgc gcataacccg tcaactgcat gatctctttc tggccacacg
     4321 gcttgatctg tagtagtgaa agcacaaatt cataacattg cgcggctggc ttggcagact
     4381 ggatgaaata ttcctggacg cgcagatcgt tctgatcgaa cagcagcaag caccacgcga
     4441 ccttgccatc gcgccccgcg cggccaatct cctgataata atgaattggg gagccgggaa
     4501 cctgaaagtg aataacgaaa cggatatccg gcttatcgat gcccatgcca agcgcgtttg
     4561 tcgagcagac caccttgtag ccattcgcca ttagcttctg ctcgatagcc aggcgcacgt
     4621 cttcatctct cttcgcatga taatattcag cctcgacgcc ctgcccctgc aaaaaggccg
     4681 cgaccatctc agcgtcatgc tgcgttgcgg tatagatgat gcctgtccca gggagacgcg
     4741 gcagcaattc cgccaggtac gccagctttt cgcgttcccc gcgcagttcc acgacgttca
     4801 ggtacaggtg cggtcgctgc atcgtgccac gcaccacctg cgcgcccgca ccgatctgcg
     4861 ccagcacatc cgcctcaacg cgcttattgg ccgtcgcggt cagtgccagc acaggcacat
     4921 tgcccggaag cgcggagagc agccgcacga tacgctggta atgcggtctg aaatcatgcc
     4981 cccaggtgga gatacaatgc gcctcgtcaa tcacgatcat gctgacgcgc atctgcgtaa
     5041 tgctgcgctg ccagtcgcta ttatccaacc gttcaggcgc gatgaaaagt atcttgagct
     5101 gcccctctat cgcccgttcc agcatagcgc ggttctcctc cggctcaaac tcagagctga
     5161 cgatgccagc cgggatgcca tacacatcgt tgcagcgctg gcactggtcg cgcatcagcg
     5221 ccttcaacgg cgaaaagacc agtgtgagat gcgggtaata caggctcgcc atctgataac
     5281 acaacgactt gccccacccg gtacgctgga tcacaagcag ccgcttcccc tgcacaagct
     5341 gctcgataat ctcccgctgc ccggcatgaa agcccgtcga aatgtggaac cgttccttga
     5401 ggagttgctc tggtgtgttg ctctgccacg tgaccgccgt agtcataaga tgtaccccct
     5461 catctttggg tgcgtgtgat agagagaaat acgcctatcg cgctattttt gcattgtagg
     5521 cttgcctgat ggaattgtca atcagccatc aattgtcacc ctgagcgcag cgaagggtct
     5581 cagtcgttga gccatgagat gcttcgctgc gctcagcatg acaaaacaag atcagtcagc
     5641 atctgtcacc ctgagcgcag cgaagggtct cagt
//
LOCUS       DASZAK010001232         4104 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_563502, whole genome shotgun sequence.
ACCESSION   DASZAK010001232 DASZAK010000000
VERSION     DASZAK010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563502"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VGT44_21670"
     CDS             complement(<1..1104)
                     /locus_tag="VGT44_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583484.1"
                     /translation="MKKTRRSPFKPVSSTRRGRFSPLILIGAGVTLIIALVGAAVFVV
                     PRIVSHADTINMDCTLIVPRHPLTAKGLATPYQLTATNADNGPCNEGNPKQASFVQGA
                     VIDTVTGQISIYNPLVIDQGTKPAIMPVLPKLPLHSVVALWFGSNANTLTLQDNGGSL
                     RQGNCVNGLEDSIFGQFSYCNAPVFFQAANTAIQAGKLKIPALGTGRDGMTCPTTRDF
                     SIVDQDQSDNVTTAYIVVASGQTAQDTPANTAALQGQSVAKNGSDERLLTLVDSAIGC
                     TPWAVPDLASAGQAQMLTGLPLNELQAAANQGAPIALVPSHDPMVLVNNHSNLDKINA
                     YRAGVDQPIAQNLGAASTQQYCQNLLDIGPARLQ"
     gene            1579..3156
                     /gene="purH"
                     /locus_tag="VGT44_21675"
     CDS             1579..3156
                     /gene="purH"
                     /locus_tag="VGT44_21675"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV2583485.1"
                     /translation="MRAIISVDNRVGLAELGRALQARNVDIFSTTGTANALHEAGVNA
                     QPISTLTNFPEILGGRVKTLHPAVFGGILARRDNQDHVRELQEHHLVQIDVVVVNLYP
                     FVETVARQDVTLHEALEQIDIGGVSLIRAAAKNFEDVLVLVRPQDYAPVMQEWEETGQ
                     VSFETRRRLAAIAFQHIAAYDTAIAGYLRVQAAEPETFPEDLTFSLRRIQSLRYGENP
                     HQQAALYTWNKPSHAGGQVAKATITGAEVLHGKELSFNNLLDLDAALAAASSFSAPTV
                     VIVKHTNPCGLACDDTVVDAYKKAHAGDPISAYGGIIGCNRTVDADTANEINRLFYEA
                     IVAPEYTPEALAILSQKKNIRLLATHAPLEPGSLTRQQGQRLDVRSLSGGLLVQTLDV
                     LGEQEVAYRVVTVREPTLEEATDLMFAWKVVKHVKSNAIVLAHKLAVLGVGAGQMNRV
                     ASVHLALERAGERARGAVLASDAYFPFSDGVEAAARAGITAIIQPGGSIRDEESIRMA
                     NRYGIAMIFTGQRHFRH"
     gene            complement(3161..3571)
                     /locus_tag="VGT44_21680"
     CDS             complement(3161..3571)
                     /locus_tag="VGT44_21680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583486.1"
                     /translation="MNRGLSKIVGSAILTVLWILCFIYIRPTLVIDFGAGIITNFKFF
                     VILVGLLIIILYHIFYRSSPEATKLSLTVFLTISWIALVFFYPFQPLQGTEAEAAAGI
                     EYDGAVGFFALIGGLAICVLWVRFFSDEISLRDE"
     gene            complement(3741..>4104)
                     /locus_tag="VGT44_21685"
     CDS             complement(3741..>4104)
                     /locus_tag="VGT44_21685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583487.1"
                     /translation="PMINVSGITGAAPIWHEAMLVAEQGHPIRNFTDPGGLVRATVTY
                     PDGVETTDLFLPGTVPNFNTVASPTPITVNNGIAISLANNPPPPSGAPAPTPYCPSYS
                     FAFPPPPNNVPSPVPGWW"
BASE COUNT          906 a         1104 c         1222 g          872 t
ORIGIN      
        1 ctgcaagcgc gccggaccta tatccagcaa gttttgacaa tattgctgcg tactggctgc
       61 tccgaggttc tgcgcgattg gctgatctac tccggcgcga taggcgttga tcttgtcaag
      121 gttggaatgg ttattgacca gtaccatcgg gtcatgcgac ggtacgagcg cgatcggcgc
      181 gccctgattg gcggcggctt gcaactcgtt caatggcaag ccggtcagca tctgcgcctg
      241 gccagcgctg gctagatctg gtaccgccca gggcgtacag ccgattgcgc tgtctaccag
      301 cgtgagcagc cgctcgtcgc tgccgttctt cgctaccgat tgcccttgca aggcagcggt
      361 attggccggt gtatcctgcg cggtctgacc ggaggcgacc acaatatatg cagttgtgac
      421 attgtcgctc tgatcctggt caacgatcga aaagtctctc gtggtgggac aagtcatgcc
      481 atcccttccc gtgcccagcg ccggtatctt cagtttcccc gcctggattg ccgtattggc
      541 tgcctggaaa aaaacgggtg cgttgcaata actaaactgc ccaaagatag aatcctcaag
      601 cccatttaca cagttgccct gccgcaggct gccaccatta tcctgcaagg tcagtgtatt
      661 ggcgttcgag ccgaaccaca gcgccacaac cgagtgtaga gggagtttcg gcagcaccgg
      721 catgattgca ggctttgttc cctggtcgat caccagcggg ttgtaaatgg agatctgacc
      781 cgtcaccgtg tcgatcaccg cgccctggac gaaagatgcc tgcttggggt tgccttcgtt
      841 acaggggccg ttatcagcat tggtagctgt taattgatag ggtgttgcca gccccttcgc
      901 tgttaacggg tgccttggga cgattagagt gcagtccatg ttgatcgtgt cagcatggga
      961 aacgattcgc ggcaccacga aaacagccgc gcccaccagc gcaataataa gggtgactcc
     1021 tgcgccgatc aggatcagtg gtgaaaagcg cccgcgtcgt gttgatgaaa ctggcttgaa
     1081 tggactgcgt cgggtctttt tcatggtaac atctactcct tgacatcttt cgatgtatgc
     1141 tgcgaccgga caacccgttc tggctgcgga caacttcctg tgccgggcat aacgatgccc
     1201 ggctgcccac atgttgtgga agctgctcgg tcacatctgt gttcctaccg cgcgtcacac
     1261 cccggaacgt taccgtccaa acgcgcgttt gcctgtctct ttccagaggc agtaaagata
     1321 gttcgctgtg atatgcgatg aaaagttttg aaactttatg aaatcactta atacggttcg
     1381 gatattacca tagcgctaat gatgtgcttg catgtctgcc tttatttttg gtaggaaagt
     1441 accaattctg gttaaggttt cttcaccaac tctcgttgaa tatgaggtat actagggtcg
     1501 tagggacgcg attcatcgcg tcatggtgga tcatacgcgg ccgaggacga ggacgaagga
     1561 aaaggataaa ttatcatcat gcgagcaatt atcagtgtag acaatcgtgt gggattggcc
     1621 gaattggggc gtgcgctgca ggcgcggaac gtcgatattt tctcaacgac cggtacggcc
     1681 aatgcgctgc acgaggccgg agtaaacgcg caaccgatca gtacgctgac gaactttcca
     1741 gagatactgg gtgggcgtgt caagacgctg cacccggcag tgtttggcgg catcctggcg
     1801 agacgcgata accaggacca tgtacgcgaa ttgcaggagc atcacctggt gcaaatcgat
     1861 gtggtggtgg tgaacctgta tccatttgtc gaaacggttg cgcgccagga tgtcacattg
     1921 catgaggctt tggagcagat cgatatcggc ggcgtctcgc tcatccgggc ggcggccaag
     1981 aatttcgagg acgtgctggt cctggtgcga ccgcaggact acgcgccggt catgcaagag
     2041 tgggaggaga ccgggcaggt cagcttcgag acgcgccgcc gcctggctgc tattgccttc
     2101 cagcatattg ctgcatacga taccgctatc gcgggctatt tacgcgtgca agccgccgag
     2161 cctgaaacct tccccgaaga tctgaccttc tcactgcgac gcatccagtc cttgcgatat
     2221 ggcgaaaatc cgcaccagca ggccgcgctt tatacctgga acaagccatc gcacgcagga
     2281 gggcaggtcg ctaaagccac gattaccggc gcggaggtct tgcacggcaa agaactctcg
     2341 ttcaacaacc tgctcgacct ggacgcggcc ctggcagccg cgagcagttt tagcgcgccc
     2401 accgtcgtga ttgtcaaaca taccaatccg tgcgggctgg cttgtgacga tacggtggtg
     2461 gatgcctata aaaaggccca tgccggcgat ccgatctcgg cctacggcgg cattatcggc
     2521 tgcaatcgaa cggtggacgc ggacaccgct aatgagatca accggctttt ctacgaggct
     2581 atcgtcgctc ccgagtatac tcccgaagcg ctggcaattc tgagccagaa gaagaatatt
     2641 cgtttgctgg cgacgcatgc ccccttagag ccgggcagcc ttaccaggca gcagggtcag
     2701 cgcctggatg tgcgcagcct gagcggggga ctgctggtac aaacgctcga cgtcttgggg
     2761 gaacaggaag tggcctatcg cgtggtaacg gtgcgcgagc caaccttaga agaggcgacc
     2821 gacctgatgt ttgcctggaa agtggtgaaa cacgtcaagt ccaacgcgat cgtgctggcg
     2881 cacaaactgg ccgtgctggg cgttggcgcg ggccagatga accgggtggc cagcgtacat
     2941 ctggccctgg agcgagcggg ggaacgcgcg cgcggcgcgg tgctggcctc agatgcctat
     3001 ttcccatttt ccgatggtgt cgaagcggct gccagagcgg gtatcaccgc aatcatccag
     3061 ccgggcggct ccattcggga cgaggaaagt attcgtatgg cgaaccgcta cggcatcgct
     3121 atgatcttta ccggccagcg ccatttccgg cattgacgac ttattcgtct cggagagaga
     3181 tttcgtccga gaagaagcgc acccacagaa cgcaaattgc cagcccacca atcagcgcaa
     3241 aaaagccgac cgcgccatca tattcaatgc ccgcggcggc ttcagcctcc gtcccttgca
     3301 ggggttggaa cgggtagaag aataccaggg ctatccagga aatcgtgaga aatacggtca
     3361 ggcttagctt ggtggcttca gggctggaac gatagaagat atgatacagg atgataatga
     3421 gcaggcccac taaaataacg aaaaacttga agttcgtgat gatccctgcg ccaaaatcaa
     3481 tgacgagcgt ggggcgaata taaatgaagc acagtatcca taacacagtc aggatcgcgc
     3541 ttccaacaat cttgctcaat cctctattca cgacaagagg ctcctttcaa ggagtactga
     3601 cgatacaaga gacaacgggg cagcgcccca ggggaaaccg ggtctgatgt atgtattgta
     3661 gtcctaacac gtaaagaaat caactgccaa aacacatggc tctgcatcct ttaagatgga
     3721 ggcccttcta actcttttca ctaccaccac cccggaaccg gcgacggcac attattgggc
     3781 ggaggcggaa aggcgaagct atagcttggg caatagggtg tcggagcggg cgcgccggac
     3841 ggcggcggcg ggttattggc aagcgagatc gcaatgccgt tgttgacggt gatcggcgtc
     3901 ggagacgcta ccgtgttgaa gttcggcacc gtgccgggga ggaacaggtc ggtggtttcg
     3961 acgccatcgg gataggtcac agtggcgcgc accaggccgc cggggtcagt aaagtttcgg
     4021 atggggtgac cctgttcggc gacgagcatg gcctcgtgcc agatgggagc cgcgccggtg
     4081 atgccggaga cgttaatcat gggc
//
LOCUS       DASZAK010001233         3248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_281017, whole genome shotgun sequence.
ACCESSION   DASZAK010001233 DASZAK010000000
VERSION     DASZAK010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281017"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VGT44_21690"
     CDS             complement(<1..213)
                     /locus_tag="VGT44_21690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583488.1"
                     /translation="MGRFQGVNGTALHKVGAGQGLGEIALAILPRADVHFLREGTRNE
                     QNNILYLLTRNMLAHFCPDIGQQQTGN"
     gene            277..1203
                     /locus_tag="VGT44_21695"
     CDS             277..1203
                     /locus_tag="VGT44_21695"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2583489.1"
                     /translation="MKSACGIYTNRLEAEHPDTLATLNALALLYQAQRRYAEAEELML
                     RVQQIYEKKQEPLDVATSLSNLASLYNAQYRYTDAEPRLRRALEIRQKRLGEDHLDVA
                     TTKNELGLTCLAQSRYDEAETLLKSALVIRQRVLKEDDPDVATTKSILADLYQALSRY
                     NEAEPLLQEALSIREKALGKDHADVAITLSNLATLYYEQDRYEEAEPLFKQALDILKK
                     LRKEDDPDVAAILNNLGRLYYNQARYKESEPLIKQALDILQKALEEDDSQLIAIQWLY
                     VSLLRQVKKEQEAAALEKHIRQIQESAQQERG"
     gene            complement(1223..1603)
                     /locus_tag="VGT44_21700"
     CDS             complement(1223..1603)
                     /locus_tag="VGT44_21700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583490.1"
                     /translation="MDPTMLCLDALIIGAGANTRYSSITPDSTKALYNDLKTELQQRI
                     AGQPEAATLLFNWDEHCADLRLPMQEALDKAHITEDEEVVQMARKLLKWLQPQQALQR
                     GYIQHMTGNIQDQAKNEYLRIITK"
     gene            1823..3241
                     /locus_tag="VGT44_21705"
     CDS             1823..3241
                     /locus_tag="VGT44_21705"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEV2583491.1"
                     /translation="MATTGETRVERDSMGEMQVPIDAYYGASTQRAVLNFPISDLRFP
                     RAFIRALGQIKQAAAQVNEGLGLVDAQVAEAIERAAQEVIDGKLDSHFVLDIFQTGSG
                     TSTNMNANEVIANRASELLGGSRGSRKVHPNDHVNFGQSSNDVIPTAIHLSALVSVEH
                     ELIPALQGLQQALEQKADEFMPIVKTGRTHLQDATPIRLGQEFLGYAGQIERSIARLR
                     HAQNEFSEVALGGTAVGTGVNTHPAFAQRVCERLSAMNGVTVRETSNHFQAQNTLDNI
                     VEASGALNTLAVSLMKIANDIRWLGSGPRAGIGEIDLPAVQPGSSIMPGKVNPVIAES
                     VCMVCAQVMGNHTTISIAGQSGNFEINVMMPVTAYNLLQSISLLAAAARNFTEQCIKG
                     IQATTRGPEMVERGLAICTSLAPVIGYDAAANLSKEAYKVGKTIREVARERTNLTDEE
                     LDRILDPASMTKPGLEGGPAGG"
BASE COUNT          756 a          881 c          933 g          678 t
ORIGIN      
        1 attcccggtc tgctgctggc caatatccgg gcagaaatgg gcgagcatat ttcgcgtgag
       61 caggtagaga atgttattct gctcgtttct cgtgccttcc cggagaaagt ggacatcagc
      121 acgtggaagg attgccagcg ctatctcgcc caggccctgg cctgcgccca ccttgtgcag
      181 cgctgtacca ttgacaccct ggaagcggcc caattacttg atcgagctgg tcgatacctg
      241 cgcgagcgcg cgcaatatag cacggcagag ggtctattga aatccgcatg cggtatttac
      301 acgaatcgcc tggaagcaga gcatcccgac acgcttgcca ccctcaatgc cctggcctta
      361 ctctaccagg cgcaacgtcg ctacgcagag gcggaagagt tgatgctgcg cgtccagcag
      421 atttacgaga aaaaacagga gcctcttgat gtcgctacca gcctttctaa cctggcatct
      481 ctgtacaacg ctcaatatcg ctatactgac gcggaaccgc gtttgcgccg cgccctggaa
      541 atccgtcaga agcggctggg agaagatcat cttgatgtcg ctacaacgaa gaacgagttg
      601 ggtttgacct gtctcgcgca aagtagatat gatgaggccg aaaccttgct taaaagcgcc
      661 cttgttattc gtcagcgggt gttgaaggaa gacgaccctg atgtggccac gacaaagagc
      721 attttagccg acctctacca ggctctgagc aggtataacg aggctgaacc actactccag
      781 gaggcgctat ccatccgtga aaaggctttg gggaaagacc acgctgatgt agctattact
      841 cttagcaatc tggccacgct ttattatgaa caagaccggt atgaagaggc agagccttta
      901 ttcaagcaag ctctggacat acttaaaaag ttgcgtaaag aagatgatcc tgatgtagcc
      961 gctattctca ataatctggg caggctctat tataatcaag cccggtataa agagtcagag
     1021 ccgctaatta agcaagctct ggacatactt caaaaagcgc tggaagaaga tgactctcaa
     1081 cttattgcta tacagtggtt gtatgtttcc ctccttcgcc aggtgaagaa ggagcaggaa
     1141 gcggcagcgc tggagaaaca catacgacaa atccaggaga gtgcccaaca agaacgggga
     1201 tgaaagtctt gtatggtccc tgttatttag tgatgatgcg aaggtactcg tttttcgcct
     1261 ggtcctgtat atttccggtc atgtgctgga tataaccgcg ctgtaaggct tgctgcggtt
     1321 gcagccactt caggagcttg cgcgccatct gaacaacttc ctcgtcttca gtaatatggg
     1381 ctttgtccaa cgcttcttgc ataggaaggc gcaagtcagc acaatgctca tcccaattga
     1441 agagaagggt cgctgcctca ggttgtccgg caatgcgctg ctgcagctca gtcttcaaat
     1501 cgttatataa agctttcgtg gaatcggggg taatggagct atagcgagta ttcgcgcctg
     1561 ctcctattat caatgcatca agacaaagca ttgttgggtc catgatgcgc cctgaccttt
     1621 catagaatat gctctcgaat cttaaacgag ccagcattac gcaagttgca gatgactgcc
     1681 tgctacgaca gagtatgaca gaaatgtgtc acttctgtaa acgtaagtgt agcatcaaat
     1741 tgagcgtact gcaagctctg gcgaggtgcg tggtataata ccctgtacat tctacattac
     1801 ccactcgacg aggagcaact acatggccac tacaggagaa acgcgcgtcg aacgcgattc
     1861 gatgggcgag atgcaggtac cgatagatgc ctattacggc gcaagcacgc agcgcgccgt
     1921 cttgaatttt ccgatcagcg atctacgttt tccgcgcgcg ttcattcgcg cgttggggca
     1981 gatcaagcag gcggcagcgc aggtcaatga ggggctgggg ctggtcgatg cgcaggtcgc
     2041 ggaggccatt gagcgcgcgg cgcaggaggt catcgatggc aagctcgaca gccatttcgt
     2101 gctggatatt tttcagaccg gctcaggcac ctctaccaac atgaatgcca acgaggtgat
     2161 cgccaatcgc gccagcgagc tgctgggcgg gtcgcgcggc tcgcgcaagg tgcatcctaa
     2221 cgaccacgtc aactttgggc aatcctccaa cgatgtgatc ccgaccgcca tacacctgtc
     2281 ggcgctggta agcgtagaac atgagttgat cccggcgctg caaggcttgc agcaggcgct
     2341 ggagcagaag gccgacgagt ttatgcccat cgtgaagacc gggcgcacac atttgcagga
     2401 cgccacgccg atccgcctgg gtcaggagtt tctaggctat gcggggcaga ttgaacgcag
     2461 catcgcgcgg ttgcgccacg cgcaaaacga attctcggaa gtggcgctgg gcggcacggc
     2521 ggtcggcaca ggcgtgaata cgcacccggc ctttgcgcag cgcgtctgcg agcggctctc
     2581 ggcgatgaat ggcgtgacgg tgcgcgagac gagcaatcat tttcaggcgc agaatacgct
     2641 ggacaacatc gttgaagcga gcggcgcctt gaatacgctg gcggtcagcc tgatgaagat
     2701 tgccaatgat attcgctggc tcggctccgg cccgcgcgcg ggtattggcg agattgacct
     2761 gccagccgtg cagcccggca gttcgatcat gcccggcaag gtcaacccgg tgattgccga
     2821 gtcggtctgc atggtttgcg cccaggtgat gggcaaccat accaccatca gcatcgcggg
     2881 ccagtcgggg aatttcgaga tcaacgtgat gatgcccgtc acggcctata acctgctgca
     2941 atcgatcagc ctgctggcgg ctgccgcgcg caacttcacc gagcagtgca tcaagggcat
     3001 ccaggccacc acgcgcggcc cggagatggt cgagcgcggc ctggccatct gcacctcgct
     3061 cgcgccggtg atcggctacg atgccgccgc caacctctcg aaggaagcct acaaggtcgg
     3121 caaaaccatc cgcgaagtgg cgcgcgagcg taccaatctg accgatgagg aactcgaccg
     3181 catccttgac cccgcgagta tgacgaagcc gggactggag ggcggcccgg cgggaggcta
     3241 ggatactg
//
LOCUS       DASZAK010001234         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_136411, whole genome shotgun sequence.
ACCESSION   DASZAK010001234 DASZAK010000000
VERSION     DASZAK010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136411"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2135)
                     /locus_tag="VGT44_21710"
     CDS             complement(<1..2135)
                     /locus_tag="VGT44_21710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583492.1"
                     /translation="MNKMMTSWWKSLSFARWSLRRRVGQFIPIAASFVLLVVAVQSIG
                     ALHDVSSTLVQQQIARSWRDSYDLLVRPQSAVSAPERGAGWIDPQSMLETYGGIDQQQ
                     LDSISTIAHVVQVSPVAMVGWQPVDVRMPLHLPGQGVYRVTATWSNQWASDTVAHYVD
                     VTDLAHVVQDQTIASPTIEFLLPGHTPAMYVLTLQALQAIMGVSSTGENTLLPHSVSI
                     STMRLMLRVERLRGGMDSLPGCVQRQACWEAQAAQQGTSSYQIDGVQLLRYTRVQYEA
                     TSQQLAAGQVTIVPAGKDTQGLLYRVPLTTSVALPPQSADAPVLQALSPVPFSMAERS
                     SILPVTPHVIPLAEACQINGTACYSGVYVSLSDVDQYSQRSLALLQATAVAISARTGL
                     HVDILDGSSLRTITFAQASQATASLQADWRVVGVAVQIAHGVDGLQATLLVLCSIVCL
                     LSIGMAGVLVGIGRRKNALLLRQLGWQGDALALVFLCDALALCAPGCLLVVGWTIIAT
                     KAWPTSVPASIIWTLIGVGALVYCGLLVGSANLWHERFGNGKATRHERLEGNTRATAR
                     VPWYFPRHRQGRGALLRFPQSTPLIIQGVGNLAIALAVFLIVEGYVLMSGFNQALIVT
                     ILGKQVHTVLEGPQLLLLAVIIGAALLTVGFCSTLLLRGRRDEIQLLARIGWERGHVL
                     LRLMRDSCSPAMVSGGIGVLAALGVAALVA"
     gene            complement(2132..2584)
                     /locus_tag="VGT44_21715"
     CDS             complement(2132..2584)
                     /locus_tag="VGT44_21715"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HEV2583493.1"
                     /translation="MRPYTGRLNRDKTLWKSSSWLPLLLLALILTSCFGGSAQSQPDP
                     RLKVFHVLSGRIIMQDNQAEVDGTIQNSGHDPFPYDVTISATFYDGSGNVIGQAQGVA
                     EDVFPGTVGAFVLIGQVNSLRYSHMQLTLVSLRERRVEHLTPTPPVSP"
     gene            2869..3102
                     /locus_tag="VGT44_21720"
     CDS             2869..3102
                     /locus_tag="VGT44_21720"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2583494.1"
                     /translation="MKTTSSKERGVKPHGLPHLCVLRQRNGLSLGQLSDMTGLSKDTI
                     THLENGRQDPQPYQVRLLARVLNVHQPDLVSPS"
BASE COUNT          733 a          973 c          857 g          575 t
ORIGIN      
        1 gcaaccaatg ccgcaacgcc caatgccgcc agcacgccta tgcccccact caccatcgcc
       61 ggtgagcagc tatcgcgcat caagcgcagc agcacatgcc cgcgttccca cccgatcctg
      121 gcaagcaact gtatctcgtc gcgcctcccc cgcaacaaca acgtcgaaca gaaaccgacc
      181 gtcaacagag ccgcgccaat tatcactgcc agtagcagca actgaggtcc ctccaacacc
      241 gtatgcacct gcttccccag gatagtgacg atcaatgcct ggttaaaacc agacatcagt
      301 acgtatcctt cgactataag aaatacagca agagcgatgg ccaggttgcc tactccctgg
      361 atgatgagcg gtgtgctttg cgggaaccgc aagagtgcac ctctaccctg acgatgacga
      421 gggaaatacc agggcaccct tgcggtcgcc ctggtatttc cctccaaccg ttcatgcctc
      481 gtcgcctttc cgttcccaaa ccgttcatgc cacaaatttg cgctgccgac gagcagaccg
      541 cagtagacca gcgcgcctac gccgatcagc gtccatatga tcgatgcagg aacgctcgta
      601 ggccatgcct ttgttgcgat gatcgtccag cccaccacga gcaggcagcc gggagcgcac
      661 agagccaggg catcgcagag aaacaccagc gccagcgcgt caccctgcca accgagctgg
      721 cgcagaagca gggcattttt tctgcggcca atgcccacca gcacaccggc catgccaatc
      781 gaaagcaagc acacgatgga gcagaggacg agcagcgttg cctgcaaacc gtccacgcca
      841 tgcgcgatct gcactgccac tcccacaacg cgccagtcgg cttgcaggct ggcagtcgcc
      901 tgcgaagctt gcgcaaaggt gatcgtgcgc aatgaagagc catcgagaat atcgacgtgc
      961 aggccggtgc gcgcgctgat ggccaccgcc gttgcctgca acagcgcgag gctgcgctgg
     1021 ctatattggt caacatcact caggctgaca tacacgccgc tataacaagc ggtgccattg
     1081 atctgacacg cctccgccag tgggatcaca tgaggcgtga ccggcaggat cgaagagcgt
     1141 tcggccatgg aaaacgggac aggactcaat gcctggagaa ctggtgcatc cgcgctttgc
     1201 gggggcagcg ccaccgatgt tgtcagtggc acacgataga gcagcccctg tgtatctttg
     1261 cccgccggta caatcgtcac ctgcccggcg gcaagttgct gagaagtggc ctcgtactgt
     1321 acacgggtgt agcgcagcaa ttgcacgccg tcaatctgat agctgctcgt tccttgctgg
     1381 gccgcctgcg cctcccaaca tgcctgacgt tgaacgcatc ctggcaagct gtccataccg
     1441 ccgcgcagcc gttcgacgcg cagcataagg cgcatcgtgg agattgacac ggaatgaggc
     1501 aacagcgtat tctctccggt tgatgaaacg cccataatcg cttgaagcgc ctggagcgtc
     1561 aagacataca tcgcgggcgt atggccaggt aacaggaatt ctatagttgg actggcgata
     1621 gtttgatcct gaacgacatg cgccaggtcg gtcacgtcca cataatgcgc gacggtatcg
     1681 ctcgcccatt gattgctcca ggtcgcggtg acgcgataga cgccttgccc cggcaggtgg
     1741 agcggcatac gcacatccac cggctgccac cccaccatcg cgacgggtga aacctgcacg
     1801 acgtgggcga tagtgctgat gctgtctagc tgctgctggt caatgcctcc gtaggtttcg
     1861 agcatactct gcggatcgat ccagcccgcg ccgcgttcgg gcgcgctcac ggcggactgc
     1921 gggcgcacca gcaaatcgta ggagtcgcgc cagctgcgag caatctgctg ttgaactagc
     1981 gtactggaaa catcatgcag cgcgccgatg gactggacag cgaccaccag cagcacaaac
     2041 gaggccgcga taggaatgaa ctggccaacg cgacgacgca agctccaccg cgcaaacgag
     2101 aggcttttcc accaggatgt catcatcttg ttcatggcga caccggtgga gtcggcgtaa
     2161 gatgttccac acgccgttcg cgcagggaga cgagcgtcaa ctgcatatgg ctatagcgca
     2221 ggctattgac ctgcccgatc agcacaaaag cgccgaccgt gcctgggaac acatcctccg
     2281 cgacaccctg cgcctggccg atgacattac cggaaccatc gtagaaggtg gcgctgatgg
     2341 tcacgtcata gggaaaggga tcatgcccgc tattctggat cgtgccatcg acctccgcct
     2401 ggttatcttg cataataatg cgtccggata ggacatgaaa gaccttcaaa cgcgggtcgg
     2461 gctgcgactg cgcagaaccg ccaaagcagg atgtcaggat cagggcaagt aagagaagag
     2521 gcagccagct agaagatttc catagtgttt tgtcacggtt caatcgaccg gtgtaggggc
     2581 gcaattcatt gcgcccaggg aagacacatc acccggaacg agggcgcaat gaattgcgtc
     2641 cctacaccgg tcgattgaac cttgacagaa cactagccct tttttgtcac cgttttgaca
     2701 ttgcagcgcc attgcttcca tgctatgata tgatgtaatc aaggtaatgt gggggcatga
     2761 ccatcagggt cgcccgttca tcaaactatc gtcctattca tgcacagcgc cacaccagca
     2821 atgacgcttg gactatgaat ggcgctcaac ctcaaaggag aggattacat gaaaacgacc
     2881 tcctcgaaag agcgaggggt taagccacat ggcttgcccc atctgtgcgt cctccggcaa
     2941 cgcaacggcc tgagtctcgg acaactctcc gacatgaccg ggctaagcaa agacaccatc
     3001 acccacctgg aaaatggtcg ccaggacccg cagccctacc aggtccgcct cctggcgcgc
     3061 gtcctcaacg tccaccaacc cgacctggtt tccccgtcgt aataatgcga tgatgaattt
     3121 tcaataatta atgggcta
//
LOCUS       DASZAK010001235         6269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_66275, whole genome shotgun sequence.
ACCESSION   DASZAK010001235 DASZAK010000000
VERSION     DASZAK010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66275"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1031
                     /gene="waaF"
                     /locus_tag="VGT44_21725"
     CDS             <1..1031
                     /gene="waaF"
                     /locus_tag="VGT44_21725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEV2583495.1"
                     /translation="TRALKRTYPQAEIDLLALPSSAKIVEGDPDLASIITYDPNVWRR
                     PKALFRAGNWREALRLVQRLRGRRYDLAVSVFGNWAAILAVLSGARRTVGFKDEGYPG
                     FMTDDVPGRHWKAGERLHEVDYCLQLARAAGVMPTPQDREPRLYVTEQASQQIEALLA
                     SAGIQPGKALIACHVSSNNGHSKRWPLPYWAALLERLIREDEAQIVLTGAPVDLPYIE
                     NVTRRMSEQTAGKVLNLAGKTSLGQLAALLQRADLLISGDSGPMHIAAAVGTPLIAIH
                     GPTDPAQSGPISPRATVLRSDIWCSPCYQAKDTADCRFFTTLCMKDITPNQVYHAIMQ
                     KQVVPHIQ"
     gene            1054..1872
                     /locus_tag="VGT44_21730"
     CDS             1054..1872
                     /locus_tag="VGT44_21730"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2583496.1"
                     /translation="MILQNLAMPDLAEAIEANFTEEMACFGRARSQWELHKTPELYWL
                     YCGPGELNGVLLASFASDDDTYVHAYIDEMLDFFTSRHTSFDWTTGPSTRPAHLASLL
                     EAHRFVQVDQTVGMAVDLRALNERMHVNTKLTITEVENLDALHTLCLVEQHGFGLSEE
                     KGQRFYTTYANAGFGKGLPWHHYIGWLNDTPVASASLLYHAGVAGIYGISTIPAARRQ
                     GVATTMTLHALREARTTGYRVAVLSPTDMSEALYRRIGFQTYCTLVHYEWVLGD"
     gene            1883..3073
                     /locus_tag="VGT44_21735"
     CDS             1883..3073
                     /locus_tag="VGT44_21735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEV2583497.1"
                     /translation="MPTSTFTLPPDARILVVKLATIGDLLLATPALRALRETYPRARI
                     DLLVTPASAGLLDGWEVIDNVIVLDKYLFDYPQQLLKNPRNMLKLRPLWHNLRDGKYD
                     VVILMHHLTLFFGRLKHQVLMRATGARWRVGLDNGHGWFLNVRVKDGGFGAMHEAEYC
                     LALAEALGASTNDRRLTLPLSEAERQQARELVCGQDPSGAVGEATPPTAHPIIALHPG
                     SGGYSTARRWSPRHFAQLADTLYREFGGQLLLVGGPEEAELHQQIIAMLRTAMPARSL
                     AGKGSIKVSGAVLELADLFIGNDAGPMHLAAAVGTPTVAIFGLSNSQAWGPYTGCAPG
                     QPDRQTVVVKLDLPCMPCFYRGHDLGTPEGCMTRDCLAMLGVEPVAAAARHLLRASGI
                     GVDL"
     gene            3070..3906
                     /locus_tag="VGT44_21740"
     CDS             3070..3906
                     /locus_tag="VGT44_21740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2583498.1"
                     /translation="MILSYIQAQPLLEARKLGQAVAETSLDLGKSTARVTLNTEGVSF
                     ATGERLDWAGVEKISQDTIKCYAISAEGIQPVQIFSERTNRLCSLLPTRGAPSMLIAG
                     FVMHRIKEIDPWQHAQRMIAAISPVSGSVLDTTTGLGYTAILAARTAESVTTIELDPG
                     VQAIAQCNPWSQELFDNPKITRVIGDASEVVPTIDDGSFNRIVHDPPTFKLAGELYSG
                     AFYRELYRILKRGGRLFHYIGDPDSKASGGITRGVMRRLQEAGFSRVVRHAEAYGVAA
                     FK"
     gene            complement(3951..5213)
                     /locus_tag="VGT44_21745"
     CDS             complement(3951..5213)
                     /locus_tag="VGT44_21745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2583499.1"
                     /translation="MTNPYLIYCTPGGEIREERHLRALAFDHQPLTSADLLPLPDGVT
                     LSMMPDRLAVGLNAGGKAQSIPQSRGWAAAALLPIGYTRTHLPAYEKVPGTEPLPFFG
                     YSAVAGMHGRLYVAAVQTDDPHKWHPRAFPKRDLARLVREKQSAFPTNRVVAQHAHCA
                     LDYACPTASNLFFGRWEMAIAVSPGCNARCVGCISKQEEEDLISPQDRLTFIPTIGEI
                     VEVAVPHLEQAEEAIVSFGQGCEGEPLLQYRRIEQAIREIRARTGRGVININTNASNP
                     RWLQRLYDAGLDTLRASTISGHDETYTAYYRPIGYRFEDVKESLKRARDAGVYSSINL
                     LCFPGMIDREREVEALIAFIKETGLRLIQLRNLNIDPEVLLPRMPALESMGRAVGMRA
                     LIELLRKEAPEVEIGNFSRPVQRAEAIV"
     gene            complement(5226..5516)
                     /locus_tag="VGT44_21750"
     CDS             complement(5226..5516)
                     /locus_tag="VGT44_21750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583500.1"
                     /translation="MYRFKGFDSAQCGDSMSALESMVNAWMETERPRIRHMSQSLRGN
                     HILLSFVYEDTRELEHRVAAQTTTALPRFFDDDYVDERPTGARIPATPPPSR"
     gene            5960..>6269
                     /locus_tag="VGT44_21755"
     CDS             5960..>6269
                     /locus_tag="VGT44_21755"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2583501.1"
                     /translation="MVSKQHIIIIGAGIIGLSTAYALLHEGQRRVTVLEQATVDHERG
                     ASHGFSRLLRFEYGPDPLYTHMVRLSLDRWHQLEQATGNTLYLRSGVLTLGRKDDHCT
                     H"
BASE COUNT         1266 a         1889 c         1883 g         1231 t
ORIGIN      
        1 tgacgcgcgc gctcaaacgc acctatccgc aggcggagat tgatctgctg gccctgccct
       61 ccagcgcgaa aattgtcgag ggcgacccgg accttgccag catcatcacc tacgatccga
      121 acgtctggcg gcgtcccaag gcgttgttca gggcgggcaa ctggcgcgag gcgctgcgcc
      181 tggtccagcg tctgcgcggg cggcgttacg acctggctgt gagcgtgttt ggcaactggg
      241 cggcgatcct ggccgtgctg agcggcgcga ggcgcacggt tggcttcaag gatgaaggct
      301 atcccggctt catgaccgat gacgttcccg gtcggcactg gaaagcgggc gagcgcctgc
      361 acgaggtcga ttattgcctg caactggcgc gggctgccgg cgtcatgcca acgccgcaag
      421 atcgcgagcc gaggttgtat gtcaccgaac aggccagcca gcaaatcgaa gcgctgctgg
      481 caagcgccgg tattcagccc ggcaaagcgc tgatcgcctg ccatgtcagt tccaacaatg
      541 ggcattccaa acgctggccc ctgccctact gggccgcgct gctcgaacga ctgatacgcg
      601 aggatgaggc gcagattgtg ctgaccggcg cgcccgttga cctgccctat atcgagaatg
      661 tcacgcggcg tatgagcgag cagaccgccg gcaaggtcct caacctggcg ggcaagacca
      721 gcctgggaca gcttgccgcg ctcctgcaac gcgccgacct gctcatttcc ggcgacagcg
      781 gcccgatgca tatcgccgcc gccgtcggta cgccgctgat cgccatacac ggcccgaccg
      841 atcccgcgca atcaggcccg atcagcccgc gcgccaccgt cctgcgcagc gacatctggt
      901 gcagcccatg ctatcaggcc aaagacaccg ccgattgccg ctttttcacc acgctgtgca
      961 tgaaagatat cacgccgaac caggtgtatc atgctataat gcagaaacag gtggttccgc
     1021 atattcagta agaaaaggcc agggaaatac tgtatgatcc tgcaaaatct agcaatgccc
     1081 gacctggctg aggctatcga agcgaacttt accgaagaaa tggcctgctt cggtcgcgct
     1141 cgaagccagt gggaactcca taaaacgccg gaattatact ggctctactg cggaccaggc
     1201 gaactgaatg gcgtcttgct tgccagcttc gccagcgacg atgataccta tgtgcatgca
     1261 tatatcgatg agatgctcga ctttttcacg tctcgacaca catcgttcga ttggacgacc
     1321 ggcccctcta cccgaccggc acacctggca agcctcctcg aggcacaccg gtttgtccag
     1381 gtagaccaga cggtcggcat ggccgttgat ctgcgggcat tgaatgagcg aatgcatgtg
     1441 aacacaaaac tcaccatcac tgaagttgag aacctggatg ccctgcatac cctttgcctg
     1501 gtcgaacagc acggttttgg tttgtcagaa gagaaagggc agcgctttta tactacctat
     1561 gcaaacgcgg ggtttggcaa gggtttgccc tggcatcact atatcggctg gctgaacgat
     1621 acgccggtgg catcggcctc cctgctctat catgcgggcg tcgccggtat ctatggcatc
     1681 tcgaccattc cagcggcgcg gagacaggga gtggcgacga ctatgacgct tcacgcgctg
     1741 cgcgaggcgc gcactaccgg ctaccgggta gcagtcttat cgccgaccga catgagcgag
     1801 gcattgtacc ggcgtatcgg ctttcagaca tattgcacac tcgtgcatta cgaatgggtg
     1861 ttgggggatt aataaacact acatgcctac atctacgttt accctgccgc cggacgcgcg
     1921 catcctggtc gtcaaactgg cgacgatcgg cgatttactg ctggccacgc ctgccttgcg
     1981 cgccttgcgc gagacctatc cacgagcgcg catcgatctg ctcgtcacac ccgcctcggc
     2041 gggtctgctc gatggctggg aagtcatcga caacgtgatt gtgctggaca aatatctctt
     2101 cgactacccg cagcaattgc tcaaaaatcc gcgcaacatg ctcaagctgc gcccgctctg
     2161 gcacaatctg cgcgatggaa agtacgacgt ggtgatcctg atgcatcacc tgacgctctt
     2221 ctttggccgc ctgaagcacc aggtcttgat gcgcgcgacg ggggcgcgct ggcgcgtcgg
     2281 gctggacaat gggcatggct ggttcctcaa cgtgcgcgtc aaagacggcg gcttcggagc
     2341 gatgcacgag gcggagtatt gcctcgcgct ggcggaagcg ctcggcgcat cgaccaatga
     2401 ccggcggctg acgctgcccc tcagcgaggc ggaacgccag caggcgcgcg agctggtctg
     2461 cgggcaagac ccatccggcg cggtgggcga ggcgactcca cccacggcgc atccgatcat
     2521 cgccctgcac cccggcagcg gcggctatag cacggcgcgc cgctggtcgc cgcgccattt
     2581 cgcgcaactg gccgacacgc tgtaccgcga attcggcggc cagttgctcc tggtgggcgg
     2641 gccggaagag gcggagctgc accagcagat catagccatg ctgcgcacgg cgatgccggc
     2701 gcgcagtttg gccgggaagg gcagcatcaa ggtcagcggg gccgtgctgg aactggccga
     2761 cctcttcatc ggcaacgatg ccgggccgat gcaccttgcc gccgccgttg gcacaccaac
     2821 cgtcgccatc ttcggcctgt cgaattccca ggcatggggg ccgtataccg gctgcgcgcc
     2881 agggcaaccg gacaggcaga ccgttgtcgt caagcttgac ctgccctgta tgccctgctt
     2941 ctatcgcggc cacgacctcg gcacgccgga aggctgcatg acgcgcgact gcctggccat
     3001 gctcggcgtt gagccggtag cggcggcggc gaggcacttg ttgcgcgcgt cgggaattgg
     3061 agtagatctg tgatactctc ctacatccag gcgcagccac tactggaagc gcgcaagctc
     3121 ggccaggccg tcgccgaaac ctcgcttgac ctcggcaaaa gcaccgcccg tgtcaccttg
     3181 aacactgaag gcgtgagctt tgcgacgggc gaacgcctgg attgggccgg cgttgaaaag
     3241 ataagccagg ataccatcaa atgttacgcg ataagcgccg aaggaataca gcctgttcag
     3301 atcttctctg agagaacgaa ccgcctatgc agcctgctgc caacgcgcgg cgctcccagt
     3361 atgttgatcg ccggcttcgt gatgcatcgc atcaaagaga tcgatccctg gcaacatgcg
     3421 cagcggatga tcgcggcgat ctcgccggtg agcgggtctg tactcgacac gacgaccggc
     3481 ctgggctata cggccatcct ggcggccaga accgctgaga gcgtgacgac aatcgagctt
     3541 gatccgggcg tccaggccat cgcgcaatgt aatccctggt cacaggaact cttcgacaac
     3601 cccaaaatca cgcgggtgat cggtgacgct agtgaagtcg tgccgaccat cgacgacggg
     3661 agctttaacc gcatcgtcca cgacccgccg acctttaagc tggccggtga actgtactcc
     3721 ggcgctttct atcgtgaact ctatcgtatc ctcaagcgag gcgggcggct cttccactat
     3781 atcggcgatc ccgatagtaa ggccagtgga ggcatcacgc gcggcgtcat gcggcgctta
     3841 caggaagctg gcttctcgcg cgtcgtgcgg cacgcggagg cgtatggggt ggcggcgttt
     3901 aaatagcgaa caggaaggaa atcgcctgga aaaagtcgct ggctgcccgc ctatacaatc
     3961 gcctcagcgc gctgcaccgg cctggagaaa ttgccaattt cgacctccgg cgcttcttta
     4021 cgaagcagct cgatcagcgc gcgcatcccg acggcgcggc ccatgctttc tagcgcgggc
     4081 attcgcggca gcagcacctc cgggtcgatg ttcaggttgc gcaactggat caagcgcagg
     4141 ccggtttcct tgatgaaggc gatcagcgct tcgacttcac gctcgcggtc gatcatgccg
     4201 gggaagcaga gcaggttgat ggagctatag acccccgcgt cgcgcgcgcg tttgagcgac
     4261 tctttgacat cctcgaagcg atagccgatg gggcgatagt aagcggtata ggtctcgtcg
     4321 tggccggaga tcgtgctggc gcgcagggta tccagcccgg catcgtagag gcgctgcagc
     4381 cagcgcgggt tgctggcgtt ggtgttgatg ttgatgacac ccctgccggt gcgcgcgcgg
     4441 atttcacgga tggcctgctc gatgcggcga tattgcagca gtggctcgcc ctcgcagccc
     4501 tggccgaagc tgacgatggc ctcctccgcc tgttccaggt gcggcaccgc cacctcgaca
     4561 atttcgccga tggtcgggat gaaggtcagg cgatcctgcg gcgagatcag gtcctcctct
     4621 tcctgtttgg agatgcagcc gacacagcgc gcgttgcagc cgggcgagac cgcgattgcc
     4681 atttcccagc ggccaaagaa gaggttgctg gcggttggac aggcataatc aagcgcgcag
     4741 tgggcgtgct gcgccacaac gcgattggtg ggaaaggcgc tctgcttctc gcgcaccagg
     4801 cgagcaaggt cgcgttttgg aaacgcgcgt ggatgccatt tgtgcggatc gtctgtctgc
     4861 acggcagcca cgtacaggcg gccatgcatt ccggccaccg cgctgtagcc gaaaaatggc
     4921 agcggctcgg tgccaggcac tttttcgtag gcgggcaggt gcgtgcgcgt atagccaatc
     4981 ggcagcaggg cagccgccgc ccagccgcgc gattgcggga tgctttgcgc tttgccgcca
     5041 gcgttcaggc caacggccag gcggtccggc atcatcgaca gcgtcacgcc gtcgggcagt
     5101 ggaagcaagt cggcggaggt gagcggctgg tgatcgaagg caagcgctct caggtggcgc
     5161 tcttcgcgaa tttcgccgcc gggcgtgcaa taaatcagat acgggttggt catggatatc
     5221 ctgtattagc gtgacggagg gggagtcgcc ggaatgcgcg ctcccgttgg ccgctcgtct
     5281 acataatcat catcgaaaaa acgcggcagg gcagtcgtcg tctgagccgc aacgcgatgc
     5341 tccagttcac gtgtgtcctc gtagacgaag ctcaacagaa tatgattgcc gcgcaaactc
     5401 tgactcatat gccggatacg cggtcgctcc gtttccatcc aggcattgac catcgactcc
     5461 agcgcagaca tgctatctcc gcactgagcg ctatcaaagc ccttgaacct gtacacgcgc
     5521 gtttcctttc gactccacat tgttcgctca aatgtatcgc ctattgtatc attatcccgc
     5581 gcattttacc agacaagatc aaaagaactt gccctatctc gtcaccaaca tttgccggtt
     5641 tatccaacga aaacgataga caaggccgcc acaagtgcat aacaatatat gatgcaatta
     5701 gagtacattt taattttatg taattatcta tgtaatttgt ggattttatt gacattaagt
     5761 ttaatttctg gtatactcaa ccatgtcatc atcactattg tcgcagaaaa atgtgggttt
     5821 ggttgcaggg acgcgatgtt gagatttcac aaacaacata gtcaagcttc atttgtgtac
     5881 tacgagatgt ccgatcttgg tcgtgtaaac tcatcatcgc gtcccgacga ccaaacagca
     5941 ctatgggaga gttcttcgta tggtcagcaa gcagcatatc atcatcattg gagcaggcat
     6001 tatcggtctc tcaacggctt acgcgcttct acacgagggg cagcggcgcg tgaccgtact
     6061 ggaacaggcg acggtcgatc acgagcgcgg cgcttcgcat ggcttctcgc gactgctgcg
     6121 cttcgaatat ggtcccgacc cactttacac acacatggtg cggctcagcc tggatcgctg
     6181 gcatcaactg gagcaggcga ccggcaacac gctgtatcta cgctcaggtg tattgacgct
     6241 aggcaggaaa gacgatcatt gcacacacg
//
LOCUS       DASZAK010001236         5296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_173445, whole genome shotgun sequence.
ACCESSION   DASZAK010001236 DASZAK010000000
VERSION     DASZAK010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173445"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1345)
                     /locus_tag="VGT44_21760"
     CDS             complement(92..1345)
                     /locus_tag="VGT44_21760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583502.1"
                     /translation="MTAAPLLQRLVPIRRARKRTHTPKRRIALRLRIALISAALALVG
                     CFTLVLFINTIALGSFPQIIRVNPAFIVKIVERHSLPHGISIPNNGANFLQLNPLERA
                     LLLELRTISLIGFGLVALVTGVGAYWLAGYSLRPVQRVSEAARRISASTLNTRLALDG
                     PNDEIKELADTFDAMLERLQLTFDSQGRFMGDVAHELRTPLASLRTNLEVVSSDPQAT
                     LEDYREMSAAQERALTRLERLVADLLILARSEQPPVRASVALGPLLEEVCSDLQCAAA
                     SHRITLELQSDLDVMVRGDSTLLARVFSNLVENGIHYNHPGGKVTLSLDQKQSLAIVL
                     IVDTGIGIPAEQQTRIFERFYRADSSHTCHQGGVGLGLSIVAAITQQHGGQAQVESAP
                     GEGSIFTIVLPIESTPTASPASSAT"
     gene            complement(1342..2037)
                     /locus_tag="VGT44_21765"
     CDS             complement(1342..2037)
                     /locus_tag="VGT44_21765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583503.1"
                     /translation="MRLLIVEDEHDIANALVRGLRREGYAVDVAYDGMEGWRLAEIND
                     YDLLILDLNLPQMDGLEVCRRLRQARPLLLILMLTARSQPDERVRGLDNGADDYLVKP
                     FHFAELTARVRALLRRDLRVRAPLLEFADLKLDPTARVVWQRNRRVELTSKEFGILEY
                     LIRRQGEVISQETLLEHVWDREANPLTNTVRVHINSLRRKLEGTTDMPRYIETVVNQG
                     YRLGIPAPVEISS"
     gene            complement(2407..3600)
                     /gene="menA"
                     /locus_tag="VGT44_21770"
     CDS             complement(2407..3600)
                     /gene="menA"
                     /locus_tag="VGT44_21770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HEV2583504.1"
                     /translation="MQDRDSQQSVPSTRNKPKTSAQIVTAESVQAEEVPTIPIGSMQT
                     VNALQPEIAIHDISSTTSMRLPAPLIVHPEEYRRSPGEWLRIWWNGIRPSYLPLSLLP
                     LLLGSALAWIQSITPASLRGNFHPQRFVALLLAILLLQIGAHLVNDYYDFVHGIDTTN
                     SLGPGGLIQQGFIKPVSVLTLGLVMLGIGALCGLLVLTATGSVLLLTIILFGLFCAYF
                     YSATSHSLSSIALGELISYLLFGPLLTMSAYLVQTGQINYTVLLYSLSPGLLAAAFIH
                     LNNMRDAESDAQAGKHTLASLLGLRLSRLIYLLLLFGAYAPIIALGLPHHGPHLILIT
                     LWTLPGLVVLISGVTRTDAPASLHLHMQKTLKLEALFILLLLAALIITAYWPLAHLPS
                     ISLPF"
     gene            complement(3691..4329)
                     /locus_tag="VGT44_21775"
     CDS             complement(3691..4329)
                     /locus_tag="VGT44_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583505.1"
                     /translation="MTEQIDTIGQEAQQYFTLKHAARERALPKSRATIRACANSIRAS
                     HRQEFETAGDLLRQAAALLAEMAQDLRAHQDIFYAGFVQDAQKEYAEAATFTALAQNR
                     PMPLAPDLTIDWAPYLNGLGEAIGELRRYVLDQLRRGNYDSCEMILRHMDDIYALLTT
                     IDYPDAITGGLRRTTDAARGILEKTRGDLTVAVSQAQLQRAMLALQRDLKER"
     gene            complement(4417..4902)
                     /locus_tag="VGT44_21780"
     CDS             complement(4417..4902)
                     /locus_tag="VGT44_21780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583506.1"
                     /translation="MNINEQLEHLLANLGVQMEYSEDEQRGDKSEVPGERDSHHLDEI
                     AGAEPEISIFQSSRDHYGVFYRLDIIESMPQLRIMVPVEHGALKMEMYLVRLNSQATL
                     HNPFASIVAYHGSTAFERSREASLQHLLYAAAEMMKQLFWAGDLDSMTFPPEIEVRRL
                     V"
BASE COUNT         1149 a         1414 c         1612 g         1121 t
ORIGIN      
        1 gtcatggtaa gggcgtaccc ttgcggtcgc ccttccaagc gtggctgcta tcagttattc
       61 tcattgtagc aaaagaggaa taaaatggct gttaagttgc ggatgaggcg ggtgaagccg
      121 ttggggttga ttcgatgggc agcacaatgg tgaagatgct gccctcaccg ggggcgcttt
      181 ccacctgcgc ctggccgccg tgttgctgtg tgatggcggc tacgattgag aggcccagtc
      241 ccacgcctcc ctggtggcag gtgtgggagc tatccgcgcg gtagaaacgc tcgaagatgc
      301 gggtctgttg ctctgcgggg atgccgatac cggtgtccac aatgagcaca atggccagtg
      361 actgcttctg atcgagggag agcgtgactt tgccgccggg atgattgtag tggatgccgt
      421 tttcgaccag gttgctgaag acgcgcgcca gcaaggtgct atcgccgcgt accatcacgt
      481 ccaggtcgct ttgtaattcc agcgtgatac ggtgtgaagc ggcggcgcat tgcaggtcgc
      541 tgcaaacttc ttccagcaag ggaccgagcg cgacactggc acggacgggg ggctgctcgc
      601 tgcgcgccag gatcagcaga tcggccacga gacgttccag gcgcgtcagg gcgcgttcct
      661 gggcggcgga catctcgcga taatcttcca acgtggcctg tgggtcggac gagacaacct
      721 ccagattagt gcgcagcgag gccaggggcg tgcgcagttc gtgggcgaca tcgcccataa
      781 agcggccctg gctatcaaag gtgagttgca agcgttccaa catggcatcg aaggtgtcgg
      841 ccagttcctt gatttcatca ttcggcccat caagtgccag gcgtgtattg agcgtactgg
      901 cgctgatgcg ccgcgccgcc tcgctgacgc gctgtaccgg gcgcagtgaa tagcccgcca
      961 gccagtacgc gcccacgccg gtaacgaggg cgaccagccc gaaaccgatc aacgaaatag
     1021 tgcgcagttc aagcagcagc gcgcgttcca ggggattcag ttggaggaaa ttggcgccgt
     1081 tgtttggaat ggagatgccg tgcggtagtg agtgtcgctc tacgatcttg acgatgaagg
     1141 ccgggttgac gcggataatc tgggggaagg agccgagcgc gatagtattg ataaagagca
     1201 ccagcgtgaa acaaccgacc agcgccagcg ccgcggagat cagcgcgatg cgcaggcgca
     1261 gcgcgatgcg acgtttagga gtatgagttc gtttgcgggc gcgccggata ggaacaagac
     1321 gctggagcag tggggcagcg gtcatgatga aatctccacg ggagccggaa tgccgaggcg
     1381 atagccctgg ttgacgacgg tttcgatgta gcgcggcatg tctgttgtgc cttcaagctt
     1441 gcggcgcagc gaattgatgt ggacgcgcac ggtgttggtt aacggattgg cctctctgtc
     1501 ccagacatgt tcgagcaatg tctcctggct gataacctcg ccctgccggc gaatcaaata
     1561 ttccagaatg ccaaattcct tgctcgtcag ttcgacgcga cgattgcgct gccatactac
     1621 gcgcgctgtc ggatcgagct tgaggtcggc gaattccagc agaggcgcgc gcacacgcag
     1681 gtcgcgccgc agcagtgcgc gcacacgcgc ggtcagctcg gcgaagtgga acggttttac
     1741 caggtagtcg tccgcaccgt tatcaagccc gcgcacacgc tcgtccggct ggctgcgcgc
     1801 cgtgagcatc agaatgagca gtaggggtcg cgcctggcgc aggcggcgac agacctccag
     1861 cccgtccatc tgcggcaggt tgaggtcgag gatcagcagg tcgtagtcgt tgatctcggc
     1921 cagtcgccaa ccttccatgc cgtcgtaggc aacatccacc gcatatcctt cgcgccgcag
     1981 cccgcgtacc agtgcgttgg cgatatcatg ctcgtcttca acaattagca gtctcatgcg
     2041 ttgttatcct gttggtatgc cccattcaca ccatcgtggc cgataatgag tgttcacgac
     2101 caggatcgga catctcgtag gagccgattg atcgcgctcg cagccgattg atcggccagt
     2161 cctaacccat gccagggatt cgcgccgatc aatcggctcc tacgagatat taccgatgtt
     2221 gtttgtaaaa aatcataatc ggcacctacg attaccctac caccgaaacg tatgcctggg
     2281 ttcaagattc tgtaaaaatt tggtaaaacg acgcggagcg tcaagtctat tataccaaaa
     2341 gtgatatgat ggcatggcga tcccttgcgg tcgccatgct gggatgtaaa tggctctgat
     2401 tggtacttaa aacggcagcg aaatggaggg aagatgcgcc agcggccagt aggcggtgat
     2461 aatgagcgcg gccagcagca gcaggatgaa cagtgcctcc agcttcaggg ttttctgcat
     2521 gtgcaggtgc agcgaggcgg gcgcgtctgt gcgcgtaacg ccgctgatga gtacaaccag
     2581 gccgggtaat gtccagaggg tgatgaggat caggtgaggc ccgtgatgtg gcagacccag
     2641 ggcaatgatg ggagcataag cgccgaacag gagcaaaaga taaatcaggc ggctcaggcg
     2701 caggccaagc agggatgcca gagtgtgttt accggcctgg gcatcgctct ccgcgtcgcg
     2761 catattgttc aggtggatga aggctgctgc cagcagacca ggcgaaaggc tgtaaagcaa
     2821 cactgtatag tttatctgac ccgtttgcac caggtaagcg ctcatggtga gcagcgggcc
     2881 gaagagcagg tagctaataa gctcgcccag ggcgatagac gatagtgagt gagacgttgc
     2941 actgtagaaa taggcacaaa acagtccgaa caggatgata gtaagcagca gcacgctgcc
     3001 agtcgcggtc agcacgagca gcccgcagag cgcgccgatg ccaagcatga ccagtcccag
     3061 ggtcagcacg ctcaccggct tgatgaatcc ttgctggatc aaccctccag gccccagcga
     3121 gttagtagta tcgataccat gtacgaaatc gtagtagtcg ttgacgagat gcgctccgat
     3181 ttgtagcaag aggatggcca gcagcagagc aacaaagcgc tgcgggtgaa aatttccgcg
     3241 cagggatgcc ggagtgatac tctggatcca ggccagcgcg ctgccaagca ggagcgggag
     3301 gagcgagagt ggcaggtagg atggccggat gccattccac cagatgcgca gccattcgcc
     3361 agggctgcgg cggtattctt ctggatgcac gatcaggggc gcgggaagac gcatggaggt
     3421 tgtggaagaa atatcatgaa tggcgatctc gggctgaagc gcgttgacgg tctgcatgga
     3481 accgattggg atagttggca cctcttcagc ctgcaccgat tcagccgtga ctatttgcgc
     3541 ggaggtcttc ggcttattgc gcgtcgaagg cacactctgc tgcgaatcgc gatcttgcat
     3601 atgttcctca tcatctctat ctgggggata gatagattat gcccctcaac ataattaacg
     3661 tttcatatta aagaaccgtt ccgccgccat ttatcgctct tttagatcac gttgtagcgc
     3721 aagcatagct cgttgtaatt gcgcctggct caccgcaaca gtcaggtcgc cgcgtgtttt
     3781 ctccaaaatg ccgcgagctg cgtcggtagt acggcgcaag ccaccggtaa tggcatcggg
     3841 atagtcgatg gtagtgagca gagcgtagat atcgtccatg tgccgcagga tcatctcaca
     3901 gctatcgtaa ttgccccggc gcagttgatc cagcacgtag cgacgtaatt ccccaatcgc
     3961 ttcgccgagg ccgttcaggt agggtgccca atcgatggtg agatcgggcg cgagtggcat
     4021 gggacggttc tgagcgagcg cggtgaaagt cgcggcctcg gcatactctt tttgcgcatc
     4081 ctgcacgaaa ccggcgtaga agatatcctg gtgggcgcgt aaatcctggg ccatctcagc
     4141 gagcagcgcc gccgcctgtc gcagcagatc gcccgccgtt tcaaattcct ggcggtgcga
     4201 ggcgcgaatg ctattggcgc aggcacgaat agtcgcccgt gatttcggca gcgcccgctc
     4261 cctcgcggca tgtttcaacg tgaagtattg ttgcgcttcc tgtccaatgg tatcgatttg
     4321 ctcggtcata cactccccct ctctgcctga ttctattata ccaagtgtaa cacagcaagg
     4381 agggaatgta tacaccagaa ctgtgtcaca tctggcttag accagccgcc gaacttcgat
     4441 ctccggcggg aaggtcatgc tatccaggtc gccggcccag aagagctgct tcatcatctc
     4501 ggcagcggca tagaggaggt gctgcaagga tgcttcacga cttcgctcaa aggctgtgct
     4561 gccatgatag gcgacgatgc tggcgaaggg attatgcagc gtagcctggg aattcaggcg
     4621 caccaggtac atctccatct ttaatgcgcc gtgttcgacc ggcaccataa tgcgtagttg
     4681 cggcatcgac tcaatgatat ccaggcgata aaagacgcca taatgatcgc gcgaagattg
     4741 gaaaatggaa atctctggct ccgccccggc tatttcatcc aggtgatgcg agtcgcgttc
     4801 gcctggcacc tcgctcttgt ctccgcgctg ttcatcttcc gaatattcca tctgcactcc
     4861 taaatttgcc agcaagtgct ctagctgctc attgatattc atacactgat tcttctttct
     4921 acccctgttt gctgcggggc ctgtatccat gccttctgtt tggttattct tataactggg
     4981 cgagaaagcg gaaagtaggc catacctata aatgtgtagg agccgattga tcgtgctcgc
     5041 agccgattat gaacttttac aaccaatgtc ggccatgtca tcctgagcga agcgaaggat
     5101 ctcatggccc atcgaccgag atcctgagcg aagcgaagga tgacatggcc ggacttgatc
     5161 gttaaaactc attatcggcc tgttaacttg gccggagcga ggaggattcg tgccgatcaa
     5221 tcggctgcga gcacggtaaa ccggctcctg ctagtccgtc aaagttcaat cggcatgtag
     5281 gggcgcaatt cattgc
//
LOCUS       DASZAK010001237         4664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_529836, whole genome shotgun sequence.
ACCESSION   DASZAK010001237 DASZAK010000000
VERSION     DASZAK010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529836"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1412
                     /locus_tag="VGT44_21785"
     CDS             141..1412
                     /locus_tag="VGT44_21785"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV2583507.1"
                     /translation="MPISDNVSAATTAPGTRSAEDIRQDFPILSRLVHGRPLVYLDST
                     ASSQKPNAVIEAMSAYYETTHANVHRGVYEISEEATARMEKARVKVARFINARHSKQI
                     IFTRNTTESINLVAYSWGNANIHAGDLIVLTELEHHSNLVPWQLLAQRTGARLEFVPV
                     SDDGLLRLDVYAQLLQQQPKLVAFAHMSNVLGTITPAQEMIAQAHAAGALVLIDAAQS
                     VPHLPVDVQALDADFLCFSAHKMLGPTGVGVLYGKRDLLEAMPPFMGGGDMIRTVGLR
                     ESTWNDLPWKFEAGTPAIAEAIGLGAAVDYLNALGMKQVAAHEQEITAYAMQRLQEVP
                     GLTIYGPPASQRGGVISFTLADIHPHDLASILDQEVGVAIRAGHHCAQPLMERFGLSA
                     TARASFYVYTIKQDIDTLAQGLHKALEIFNL"
     gene            1424..1828
                     /locus_tag="VGT44_21790"
     CDS             1424..1828
                     /locus_tag="VGT44_21790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV2583508.1"
                     /translation="MSMDYREYILDHYRNPRNYGKLESPNAHAEDSNPLCGDQLAIDL
                     QVEGDQVTAVRFQGRGCAISQAAASMLSEMIEGKTVEEVIRLGKDDVLDALGIPISPA
                     RMKCAFLSLRVLHRSLALAGLERPKEIDDTDE"
     gene            1840..2574
                     /locus_tag="VGT44_21795"
     CDS             1840..2574
                     /locus_tag="VGT44_21795"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV2583509.1"
                     /translation="MRLQNKVAIVTGVSHAGQVGFALAEGFAREGASLAISSRSVERT
                     TARAQTLQAQGATVIGVPADLTSEEGAQELIAQTVKHFGRVDILVNLAGGLTKYGPSE
                     ELSLADWESELNNNLRSAFLCSRAAWPVMKQQGGGKILNFSTAGGSHGASAMMLAYNC
                     AKAGVDALTRTLAKEGKQVGIYVNAIGPGLIVTQSNIESMKPSPEELAKKWVSKEQII
                     EAALFLVSSASDGVTGAILPVQGRGI"
     gene            2583..2900
                     /locus_tag="VGT44_21800"
     CDS             2583..2900
                     /locus_tag="VGT44_21800"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEV2583510.1"
                     /translation="MTDFIKVAQVSDLEDGEMMAVEVDGEPVCIAKVEGDVFAFTDNC
                     THISGPLNEGELDGDVLTCPWHGAQFNVRTGKVLRGPARQDIQTYAVRVDNDDIMISI
                     PDA"
     gene            3000..4475
                     /locus_tag="VGT44_21805"
     CDS             3000..4475
                     /locus_tag="VGT44_21805"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583511.1"
                     /translation="MSDISSSQNLGSSKPKSSRYASYVFSIMFLISFLNYMDRNVLAG
                     AAATVAKELGFGNDGIGLLATAFIFVYAVFTIPLGIWADRAKRKNVVAICVAIWSVAT
                     ALTALATNFTTLFLSRMALGIGEAGYFPAGTALMSDYFSRRRRSRIMSWWSVGQLVGI
                     LVGFVIGGELAGIFIGSWRLAFIFTGIPGLILAFLAWKLREPRRNEADEEELALYPFT
                     VGELAEIEEPPHTIAVSAAARSQKSVAGGTIAQIKVIFSELGLLLRIRTLVVLIVMQI
                     FAFFVLGVNVIFLPTYLQQKDLFGLKSGTAGLFSGGVIVLAGLVGTVLGGYMADWLNR
                     RYQGARVLACGIGFLLSAPSFVFAITSRNFFVFSIFFVLTTILLTIYTGPSTAATQDV
                     VPSALRASSIAISLLIAHLLGDALSPAIVGILATHYDPHPILDAKLLMTGQYLSQALL
                     ITCTPALVIAGLVGVFGARWMKSDIAAAENVDRLAREAASV"
BASE COUNT          945 a         1446 c         1335 g          938 t
ORIGIN      
        1 tttctcgcgc tcccccccga tttatcggcc accattggtg tcggtgagga ggcaggactg
       61 gccgataaat cggcggtgag cgcgataaat cggctcctac ggggtattgt agggcgacgc
      121 ttgccccgga gatgaaaaag atgcccatat cggataacgt atcggcagcg acaaccgcgc
      181 cgggaacacg cagcgcggaa gacatccgcc aggattttcc catcctgtcg cgcctggtgc
      241 atggcaggcc gctggtctac ctggacagca ccgccagctc gcagaagccc aacgccgtca
      301 tcgaggccat gagcgcctat tacgagacga cacacgccaa cgtgcatcgc ggcgtctacg
      361 agatcagcga agaggcgacc gccaggatgg agaaagcgcg cgtcaaagtg gcgcgtttca
      421 tcaatgcgcg ccacagcaag cagatcatct tcacgcgcaa caccaccgag agcatcaacc
      481 tcgtggcgta tagttggggc aacgccaaca ttcacgcggg cgacctgatc gtgctgaccg
      541 agcttgagca tcacagcaac ctcgtcccct ggcaactgct cgcgcagcgc accggcgcac
      601 ggctggagtt cgtgccggtt agcgacgatg gcctgctgcg cctggatgtc tacgcccagc
      661 tcttgcagca gcagccgaag ctggtggcct tcgcccacat gtcgaacgtg ctcggcacca
      721 tcactccagc ccaggaaatg atcgcgcagg cccacgccgc cggggcgctt gtgctgattg
      781 acgccgcgca gagcgtcccg cacctgccgg tagatgtaca ggcgctcgac gccgacttcc
      841 tctgctttag cgcgcataaa atgctgggtc ccaccggcgt cggcgtcctc tacggcaagc
      901 gcgacctgct ggaagccatg ccccccttca tgggcggcgg cgacatgatc cgcaccgtcg
      961 gcctgcgcga atcaacctgg aacgatcttc cctggaaatt tgaggccggg acccccgcta
     1021 tcgccgaggc aattggcctg ggcgcggcgg tcgattacct caacgcgctc ggtatgaaac
     1081 aagtcgcggc acacgagcag gagattaccg cttacgccat gcagcgcctg caagaggtgc
     1141 cagggctgac catctatggc ccgccagcct cgcagcgcgg cggcgtcatc tccttcaccc
     1201 tggcggatat ccacccgcac gacctggcct ccatcctcga tcaagaggta ggcgtggcca
     1261 ttcgcgccgg tcatcactgt gcgcaaccgc tcatggagcg tttcggcctc tccgccaccg
     1321 cgcgcgccag cttctacgtg tatactatca agcaagacat cgatacgctg gcgcaaggac
     1381 tgcataaagc gttagaaata ttcaatctgt agggacataa cctatgtcaa tggattatcg
     1441 cgaatacatc ctcgatcatt atcgcaaccc gcgcaattat ggaaagctgg aaagccccaa
     1501 cgcgcacgcc gaggactcca atcccctgtg tggggaccag ctcgccattg atttacaggt
     1561 cgagggcgac caggtcacag ccgtgcgttt ccagggacgg ggatgcgcca tcagccaggc
     1621 ggcggcctcg atgctctcgg agatgatcga gggcaaaacc gtcgaggaag tcatacggct
     1681 cggcaaagac gatgtgctgg atgccctggg cattcccatc agccccgccc gcatgaagtg
     1741 cgccttcctt tcactgcgcg tcttgcaccg cagcctcgcg ctggccggat tggaacgccc
     1801 caaagagatc gacgacaccg acgaataaag aggcatcaca tgcgcttaca gaataaagtt
     1861 gccattgtca ccggcgtcag ccatgccggg caggtcggtt tcgcgctggc cgagggtttc
     1921 gcgcgtgaag gcgcgtcgct ggccatcagc tcgcgcagcg tcgaacgcac caccgcgcgc
     1981 gcccagacat tgcaggccca gggcgcaacc gttatcggcg tgcctgccga cctcaccagc
     2041 gaggaaggcg cgcaagagtt gatcgcgcag accgtgaaac acttcgggcg cgtcgatatc
     2101 ctggtcaacc tggcgggcgg cctgacgaag tacggaccat cggaagagct gagcctggcg
     2161 gattgggaat cggaactcaa caataacctg cgttccgcct tcctctgctc gcgcgcagcc
     2221 tggccggtga tgaaacagca gggtggcggc aaaatcctca acttcagcac cgccggtggg
     2281 tcgcatggcg caagcgccat gatgctggcc tacaattgcg ccaaagccgg cgtagacgcc
     2341 ctgacgcgca cgctggcgaa agaaggcaaa caggtcggca tttacgtcaa tgccatcggc
     2401 ccagggctga ttgtcacgca gtcaaacatc gaatcgatga aaccttcccc cgaagagctg
     2461 gcgaaaaaat gggtcagcaa agagcagatc atcgaagccg cgctcttcct ggtgtcttcc
     2521 gccagcgacg gtgtaaccgg cgcgatcttg cccgtgcagg gtcggggcat ctaagggatt
     2581 ttatgacaga ttttattaag gtagcccagg tcagcgacct ggaagacggc gaaatgatgg
     2641 cggtcgaagt agatggcgaa cccgtctgca tcgcgaaggt cgagggcgat gtgtttgcct
     2701 tcaccgataa ttgcacgcac attagcggcc ccctcaacga aggcgagctg gatggtgatg
     2761 tactgacctg cccctggcac ggcgcgcagt tcaatgtgcg caccggcaag gtgctgcgcg
     2821 gccccgcgcg ccaggacatc cagacctatg cagtgcgggt agacaacgac gatatcatga
     2881 tcagcatccc cgatgcatag cggcatatca ccccccttca catcacctgc aatctatggt
     2941 acagttgagt tgtcgaaatg gcctcgttac tctgcgtatc cccttcattg gaggcgtctg
     3001 tgagtgatat ttcttccagc cagaatctag ggtcttcaaa gccgaagagc agccgttacg
     3061 ctagttatgt cttctcgatt atgttcctca tcagtttcct gaattatatg gaccgcaacg
     3121 tgctggccgg ggctgctgcc accgttgcca aagaactcgg cttcggcaat gacggcatcg
     3181 gcctgctggc aaccgcgttc atctttgtct atgccgtttt cacaatcccg cttggcatct
     3241 gggctgaccg ggcaaaacgc aagaacgtcg tcgccatctg tgtcgctatc tggagcgtcg
     3301 caaccgccct gaccgccctg gcgaccaatt tcaccaccct ctttctctcg cgtatggcgc
     3361 tgggtatcgg cgaagccgga tatttcccgg ctggcaccgc gcttatgagc gattatttta
     3421 gccgcaggag acgctcgcgc atcatgagct ggtggagcgt cggccagttg gtcggcattc
     3481 tggttggctt cgtcatcggc ggcgaactgg caggcatctt cattggaagc tggcggctgg
     3541 ccttcatatt caccggcatc cccggcttga tcctcgcctt cctcgcctgg aaattgcgcg
     3601 aaccgcgacg caacgaggcc gacgaagaag aactggcgct ttatcccttc accgtgggtg
     3661 aactggccga gatcgaagaa ccgcctcaca ccatcgctgt ttccgcagcc gcgcggtcac
     3721 aaaaaagcgt tgcgggcgga acgatagcgc aaataaaggt catcttttca gaactgggat
     3781 tgttgctgcg catcagaacg ctggttgtct tgatcgttat gcagattttc gcttttttcg
     3841 tgctgggcgt caatgtcatc ttcctgccca cctatctcca gcagaaagat ctctttggcc
     3901 tgaaatcggg taccgccggt ctcttttcgg gcggcgtgat cgtgctggcc ggcctggtcg
     3961 gcaccgtcct gggcggctac atggccgact ggctgaaccg gcgctaccag ggcgcgcggg
     4021 tgctggcctg cggcatcggc ttcttgctca gcgcgccatc cttcgtcttc gctatcacca
     4081 gtcgcaactt tttcgtcttc agcatcttct tcgtgttgac taccatcctg ctgaccatct
     4141 ataccggacc cagtactgcc gcgactcagg atgtcgtgcc atcagccctg cgcgcttctt
     4201 ccatcgccat ctcactgctg atcgcgcacc tgctaggtga tgcgctctct ccggcgatcg
     4261 tcggtattct tgcaacacac tacgatccgc acccgattct cgatgcgaaa ctcctgatga
     4321 ccggccagta cctcagccag gcgctgctga ttacctgcac gcccgccctg gtcatcgccg
     4381 ggctggtggg agtcttcggc gcgcgctgga tgaagtccga tatcgccgca gccgagaacg
     4441 tcgaccgctt agctcgcgaa gctgcgagcg tgtaggaatg tgactcaatt cgcaaatttc
     4501 gagtaacatt ctctactgat aaaaggacat gaatatgtta taattccgcc tgagaacacc
     4561 tttgttgtag gtgtatgaaa acgctgtagg ggtgcaattc attgcgccct gggtctctct
     4621 gcgcatcggc ttggcgtgag ggcgcaatga attgcacccc taca
//
LOCUS       DASZAK010001238         3440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_136523, whole genome shotgun sequence.
ACCESSION   DASZAK010001238 DASZAK010000000
VERSION     DASZAK010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136523"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VGT44_21810"
     CDS             <1..1002
                     /locus_tag="VGT44_21810"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2583512.1"
                     /translation="TAAPVAVLPRSIYIEPTSRCNEFCQQCPRTLLSREDDRDLSFDN
                     FRRIVDQFPLLERVVLHGLGEPLLNKELPRMVHYLKERGVYVLFNSNGVALTPKRGRE
                     LIDAGLDEYRLSMDGATREMYARVRGVDAFDKIWRNVRAFVALQNELQADNPRVSLWF
                     TAMKENLPELPNLIDLAYEHGIREVHLQRLVYFEEGLAHSKQALFRRSSREELELVRR
                     CEQICLERGILFSAAGSATPLDSLVRDFGDRPWSGCQRPYTLTYITSSGNVLSCCFAP
                     FGHRSAKEYYEERVLGNLFEEPIEAIWHGERYEAFRRAFESDHPARHCSQCGLNWSY"
     gene            1004..1966
                     /locus_tag="VGT44_21815"
     CDS             1004..1966
                     /locus_tag="VGT44_21815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2583513.1"
                     /translation="MTLKIGILVPQGWRMDLVGITDPVEAYETMTRVGLEAEALGFDS
                     IWLFDHFHTVPTPTQEVTFESWTSTAALARDTKRVRIGQLVTCNNYRNPALLAKMAST
                     VDVLSHGRLEFGIGAGWHESEFLAYGYDFPDGPTRLRQMRDAVRIILKMWSDEHAVYE
                     GQYYHVNGAINQPKGVQKPHIPLMIGGSGEQVTLKLVAQYADACNITHQDNEGMARKF
                     DIIRRHCETVGRDYNTIRRSVLMNCAIAETEEEAWAKASSPLFSRNIPSGRYREQGLV
                     GTPDAIRKRLVEIEQAGAQELIMWLPDARFLTPLRMFAEACIAS"
     gene            1963..2946
                     /locus_tag="VGT44_21820"
     CDS             1963..2946
                     /locus_tag="VGT44_21820"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEV2583514.1"
                     /translation="MSKRIGWQGLPALALLVGLVACQSPEQGGVATPMPNPTARQNNY
                     GGSIQVGGLTRTYLVHLPPGQNAPMPLVLVFHGGGGSSRGMNAVTRFNSLADAQGFIV
                     VYPDGYDKSWADGRGTTAADRFGVNDVAFVSALIDQLAHTLSINARRIYAAGISNGGF
                     FTLRLGCQLATKIAAIAVVAATMPANLAPTCHPARPVPAQFYHGSADPLVPAAGGSMT
                     VGAGGDILSINDSVALWARLNGCQSAPATTMLPQSINDGTQVQRAAYAGCQQGADVVF
                     FNIIDGGHTWPGGLQYLPVSVIGRTTRNLNATNVMWAFFEAHPWGTSSYDD"
     gene            complement(3005..>3440)
                     /locus_tag="VGT44_21825"
     CDS             complement(3005..>3440)
                     /locus_tag="VGT44_21825"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HEV2583515.1"
                     /translation="AWTPLETTTTEEAVFLAPLDHVCARGRAKLLFGFDYVWEVYKPL
                     PQRMFGYYTLPILWGDRLVARFDSKLDRTTNTFVILGLWLEDEALGLDEAFAEALAGG
                     LVRFVRFLGASTLDATSIREPLLRRHACSSQEICESFHRTGA"
BASE COUNT          658 a         1106 c         1062 g          614 t
ORIGIN      
        1 accgccgcac ctgtagccgt attgcctcgc tcgatctata tcgaaccgac cagtcggtgc
       61 aacgaatttt gccagcaatg cccgcgcacg ctgctctcgc gcgaggacga ccgcgacctg
      121 agtttcgaca acttccggcg catcgtcgat caattccctt tgctggagcg cgtggtgctg
      181 cacggcctgg gtgagccact gctcaacaaa gagctgccgc gcatggtaca ctatttgaag
      241 gagcgcggcg tctacgtgct gttcaactcc aacggcgtcg cgctgacgcc caaacgcggc
      301 agggagttga ttgatgccgg actggacgaa taccgcctgt cgatggacgg ggcaacgcgc
      361 gagatgtacg cccgcgtgcg cggcgtggac gccttcgata aaatctggcg caacgtgcgc
      421 gcctttgtcg cgctgcaaaa cgagctacag gccgacaatc cgcgcgtctc gctgtggttc
      481 actgctatga aggagaacct gcccgagctg cccaacctga tcgacctggc ttacgagcat
      541 ggcattcgcg aagtgcactt gcagcggctg gtctacttcg aggagggcct ggcgcactca
      601 aagcaggcgc tgttccgccg ttcgagccgt gaagagctgg agctggtgcg gcgctgcgag
      661 cagatctgcc tggagcgcgg cattctcttc agcgcggcgg ggtcggcgac gccgctagac
      721 agcctggtgc gcgatttcgg cgaccgcccc tggtcgggct gccagcgacc ctataccttg
      781 acgtatatca cgtcgagcgg caacgtgctt tcgtgctgtt tcgcgccatt cggccatcgc
      841 agcgccaaag aatactacga agagcgcgtg ctgggcaacc tcttcgagga gccaatcgag
      901 gccatctggc acggcgaacg ctatgaagcc tttcgccgcg ccttcgagag cgatcatccg
      961 gccaggcatt gttcgcaatg cggattaaat tggagctatt aacatgacct tgaagattgg
     1021 catcctcgtt ccccaggggt ggcgcatgga cctggtgggc attaccgacc cggtcgaggc
     1081 gtatgaaacc atgacgcgcg tcgggttaga ggcagaggcg ctgggtttcg attcgatctg
     1141 gctgttcgac cactttcata ccgtgccaac gcccacgcag gaagtcacgt tcgagagctg
     1201 gaccagcacc gccgcgctcg cccgcgacac gaagcgcgtg cggatagggc aactggtcac
     1261 ctgcaacaac taccgcaacc cggcgctcct ggccaagatg gccagcacgg tcgatgtgtt
     1321 gagccatggc cgcctggagt tcggcatcgg cgcgggctgg cacgagtcgg agttcctggc
     1381 gtatggctac gacttccccg atgggccgac gcgcctcagg cagatgcgcg acgccgtgcg
     1441 gatcatcctc aagatgtggt cggatgaaca cgccgtatac gaggggcagt actatcacgt
     1501 caacggggcc atcaaccagc cgaaaggcgt acagaagccg catatcccct tgatgatcgg
     1561 cggaagcggc gagcaggtga cgctcaaact ggtggcgcag tacgcggatg cctgcaatat
     1621 tacgcaccag gataacgagg gcatggcgcg caaattcgac atcatccggc gccactgcga
     1681 gacggtcggg cgcgactaca acaccatccg ccgcagcgtg ctgatgaact gcgccatcgc
     1741 cgaaaccgag gaggaagcct gggctaaggc ttcctcccca ctatttagcc gcaacattcc
     1801 ttctggccgc tatcgcgagc aaggactggt cggcacgccc gacgccattc gcaagcgcct
     1861 cgtcgagatc gagcaggcgg gcgcgcagga actcattatg tggctccccg acgcgcgctt
     1921 cctgaccccg ctcaggatgt tcgctgaagc gtgtatcgcg tcgtgagcaa acgaatcggc
     1981 tggcaaggcc tgcccgcgct ggcattgctg gtcggcctgg tcgcctgcca atcaccggaa
     2041 cagggaggag ttgcgacgcc catgcccaac cctaccgcga gacaaaataa ctatggcgga
     2101 tcaatccagg ttggcgggtt gacgcgcacc tacctggttc accttccgcc tggccagaac
     2161 gcgcccatgc cgctcgtgct ggtcttccac ggcggcggcg gcagtagccg gggcatgaac
     2221 gccgtgaccc gtttcaatag cctggcggac gcgcagggct tcatcgtggt ctatcccgat
     2281 ggctacgaca aatcctgggc cgatggccgg ggcacgaccg ccgccgaccg cttcggcgtg
     2341 aacgacgtgg ccttcgtttc cgcgctgatc gaccagcttg cgcatacgtt gagcatcaac
     2401 gccaggcgca tctacgccgc cggtatctca aatggcggct tcttcacgct gcgcctgggc
     2461 tgccagctcg ccacgaagat cgccgccatc gccgtcgtcg cggccaccat gcccgccaac
     2521 cttgcgccca cctgccatcc cgcgcggcct gtcccggcgc agttctatca cggcagcgcc
     2581 gaccccttag tcccagccgc aggcggctcg atgacggtcg gagccggggg cgacatcctg
     2641 tccatcaacg attcggtcgc gctgtgggcc aggctgaacg gctgccagtc cgcgcccgcc
     2701 acgacgatgc tgccgcagtc catcaacgac ggtacgcagg tgcagcgcgc ggcctacgcc
     2761 ggctgccagc aaggcgcgga tgtcgtcttt ttcaacatca tcgacggcgg ccacacctgg
     2821 cccggcggcc tgcaatacct gccggtcagc gtgatcggca ggacaacgcg caatctgaac
     2881 gcgacgaacg tgatgtgggc gttcttcgag gcgcatccct ggggcacctc gtcatatgat
     2941 gattgaaagg ggtgttcagt cgaattgggc aaaaagcccg atgtccattc cggcatgctg
     3001 cggcttacgc gcccgttctg tgaaagcttt cgcatatttc ctgtgaagag catgcgtgcc
     3061 gacgcagcag cggctcgcgg atcgacgttg catccaacgt gctcgcgccc agaaacctca
     3121 cgaagcgcac cagcccacca gccaatgcct ctgcaaatgc ctcatcgagg ccgagcgcct
     3181 cgtcctccag ccacaagccc aggatgacga aggtgttggt cgtccggtcg agtttgctgt
     3241 cgaaacgggc gacgagccgg tcgccccaga gaatgggtaa cgtgtagtag ccgaacatgc
     3301 gctggggaag aggcttgtag acctcccata cgtaatcgaa gccgaacaac agtttggctc
     3361 gcccgcgcgc gcagacgtga tcaagcggtg cgaggaagac ggcctcttca gtcgtggtcg
     3421 tttccaacgg cgtccaggcc
//
LOCUS       DASZAK010001239         1941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_103985, whole genome shotgun sequence.
ACCESSION   DASZAK010001239 DASZAK010000000
VERSION     DASZAK010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103985"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VGT44_21830"
     CDS             <1..314
                     /locus_tag="VGT44_21830"
                     /inference="COORDINATES: protein motif:HMM:NF017102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV2583516.1"
                     /translation="CTWWAQYERKNENLTHMGAAMYWAAGAARRGYRVGSTPRAGATV
                     VFQPGVQGAGGAGHVAHVIAVYPGGWFLQSEMNFYWNGGGWGRVDYRYAHVGWGVAFI
                     Y"
     gene            418..1677
                     /locus_tag="VGT44_21835"
     CDS             418..1677
                     /locus_tag="VGT44_21835"
                     /inference="COORDINATES: protein motif:HMM:NF040570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV2583517.1"
                     /translation="MVSAFCTRWAECLYVEQQRVRKTYQYRLKPTPAQERALVSVLLR
                     CRTLYNVALEQRKTWWGRGQGISASYHQQKAELPVLKAACPEYTEVHSQVLQDVLLRV
                     ERTYHGFFRRVTNGEPPGYPRFQGTKRYHSFTSPQYGNGTVLDGGVLSLSKIGRIPIR
                     LHRPLQGPPKTVTIRREADGWYVSFSCAEVPTQPLPRTGCETGIDVGLKVFLVTADGE
                     QVANPRYHRKAEKQLTKAHKKVARRKKGSKRWWKAVHQCARKYQKVKRQRRDFQHKTA
                     LDLVRTYAVIYLEDLQVGNMSRRPAPKPNGNGGYLKNAASAKSGLNTSIQAAGWYAFR
                     RILVCKAAWAGKRVEAIPPAFTTQDCSGCGERVAKSLSIRMHVCPTCGLVVDRDENAA
                     LNMLVAGQQRAGQARQALTWPDTASVV"
BASE COUNT          372 a          584 c          638 g          347 t
ORIGIN      
        1 agtgtacgtg gtgggcgcag tacgagcgca agaacgagaa cctgacccac atgggcgccg
       61 ccatgtactg ggcagccggc gcagcgcgcc gcggctatcg cgttggcagc accccgcgag
      121 cgggcgcgac cgtcgtcttc cagccgggtg tgcagggcgc gggtggcgcg ggccacgtcg
      181 cgcacgtcat cgccgtctac cccggcggct ggttcctcca atccgagatg aacttctatt
      241 ggaacggggg cggctggggt cgggtagact atcggtatgc gcatgtcggc tggggcgtcg
      301 ccttcatcta ctgacgcggc tccgcagccg actttagcat accttccggc tgaagcccgc
      361 tgcctttagg catggggatg aaagccgggt gacccgtaag ggccacgcat acccgggatg
      421 gtttccgcgt tttgcacccg ttgggcggaa tgtctctatg tggaacagca gcgtgtgcga
      481 aaaacctacc agtatcggct gaagccaacg ccagcacagg agcgggcgct ggtgtcggtt
      541 ctgctgcgct gtcgcacgct ctacaatgtc gccctggaac aacgcaagac ctggtgggga
      601 cgcggccagg gcatcagcgc gagctaccat cagcagaagg ccgagttgcc ggtgctgaaa
      661 gccgcgtgcc ccgaatacac agaagtgcat tcccaggtcc tgcaggatgt cttgttgcgg
      721 gtagagcgca cgtatcacgg gttcttccgg cgcgtcacga acggcgagcc gcccggttat
      781 ccccgcttcc agggcaccaa gcgctatcac tcctttacct cccctcagta cggcaatggc
      841 actgtgctag acggtggcgt gctcagtctc tccaagatcg gacgtattcc cattcggctg
      901 catcgcccac tccaaggccc ccccaagacc gtcacgatcc gccgggaagc ggatggctgg
      961 tatgtttcgt tctcgtgtgc cgaggtgccc acccagccct tgccccgcac aggatgtgag
     1021 accggcatcg atgtgggctt gaaggtcttc ctggtgacgg cggacgggga gcaggtagcg
     1081 aaccctcggt accatcggaa ggcagaaaag cagctcacga aggcccacaa gaaggttgct
     1141 cgccggaaga agggcagtaa gcggtggtgg aaagcggtgc accagtgcgc caggaagtat
     1201 cagaaggtca aacggcaacg ccgcgacttc cagcataaga cggcgcttga cctcgtgcgt
     1261 acatatgccg tgatctacct ggaagacttg caggtgggca acatgagtcg ccgccctgcg
     1321 cccaaaccaa acggaaacgg tggctatctc aagaacgcgg ccagcgcaaa gtccggcttg
     1381 aatacgtcta ttcaagccgc ggggtggtat gccttccgtc gtatcctcgt ttgcaaggca
     1441 gcgtgggccg gcaagcgagt ggaagcgatc cctcctgcgt tcaccacgca ggactgtagc
     1501 ggttgcggcg aacgggtggc gaagagcctc tccattcgca tgcatgtttg ccccacctgc
     1561 gggttggtcg tggaccgcga cgagaacgcg gcgctgaaca tgctcgtcgc cggtcagcag
     1621 cgggccgggc aggcccgtca ggcgctaacg tggccggata cggcgagcgt cgtctgagaa
     1681 gcccccgcct ttaggcgtgg ggagcatgtc actaaactct gtgctgatgt ctgtgatacc
     1741 gtgtcgcctt gcaccctcct gaaaatcgtc aggagggtgc tgggagagcc tcgagggtga
     1801 cagcgaagcc caaccgttcc aagcggtgga tgagacgcag ctggacacgt tcgcgcgcat
     1861 gctcgtcgca gtactgcgcc cccagctcgt ggtagggttc gcggcgagtg agcatgtgat
     1921 agatcgcaac gaggaccgag t
//
LOCUS       DASZAK010001240         6391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_315132, whole genome shotgun sequence.
ACCESSION   DASZAK010001240 DASZAK010000000
VERSION     DASZAK010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315132"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..2698
                     /gene="alaS"
                     /locus_tag="VGT44_21840"
     CDS             56..2698
                     /gene="alaS"
                     /locus_tag="VGT44_21840"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV2583518.1"
                     /translation="MSVTLKLTGTEIRQKYLDFFVSKGHLRVPSSSLVPRNDPTVLLT
                     TAGMQQMIPYFLGRETPPAQRLTSAQKCFRTTDIDKVGNERSLTFFEMMGNFSVGDYF
                     KRDAIAFAWELLTKIFMIPADKLYPTVHPQDDEAPRWWSEIAGYPDETITRLEDNWWG
                     PPGASGPCGPDSEIYYDRGVEHGCGRADCAPGCDCERFLEIWNLVFMQFYQDLDGTRT
                     PLPKKNIDTGLGLERVSMVLQGKESVFDTDLFRPIIDKFASLARTTYGSNPKADTSLR
                     VIADHGRALVFLAADGVLPSNEGRGYIFRRILRRAVRHGKLLGLDKPFLSEAADTVID
                     LMKSHYVELGLQRDRIVEILSLEEKKFSQTLNTGLALLTTLLDDLKQRGKHVVPGADA
                     FKLYDTHGFPLELTQEVAAEHGFTVDITAFEQALLQQQERSRATAAFEQSTDVDPLTG
                     LLQRVGPTEFTGYQGITGSGKVVGLVVDGVEVEEISAPQSALVILDSTPFYAESGGQI
                     GDRGNITGSMGVFQVQDTQKPLKGLIVHYGRIAEGFMRVGDSVQASVNGQRREDTIRN
                     HSATHLLHKALRDMLGPQVEQRGSLVEPERLRFDFASPRALTSADLARLDEQINDWIR
                     ADYAVQTRIMPLQDALQTGAMALFGEKYDENVRVVTMGGSTELCGGTHCASTGQIGEY
                     ITIQETSISAGIRRIEALTGRGADAYLRRRVSLVDSISAKLQTQPDQLEVRVEQLAQE
                     LATARKQLAQYQREAAQQQTETLARTAREIEGVPVVATVAAVPDDKLLREMGDMVLRK
                     LTRPGVVVLAGNLDERVGLQVSVSPELVKRGIHAGKIAGAVGERLGGRGGGRPESAQG
                     GGKNKAELGAALDIVAKLVKESLK"
     gene            2833..4161
                     /locus_tag="VGT44_21845"
     CDS             2833..4161
                     /locus_tag="VGT44_21845"
                     /inference="COORDINATES: protein motif:HMM:NF025806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division FtsA domain-containing protein"
                     /protein_id="HEV2583519.1"
                     /translation="MNNPLQRIRNFITGRTTGNSVEYERDGHAHSADDDQRQLFTALD
                     IGTAYAKALIIEVQDEIAIVLGAGRNAQSYHHMSDGIVIDIHSVIENCNQALLKAEEA
                     AGGVVAPSTIIGIAGEMVKGSSTTVSRQRQDPMKLITPEELEGMITSAQKKLHKEASA
                     RIAAETGYSNVEVRLTNASVISARIDGQSVHNPVDFRGRNLELTIFSSFAPLMQLGAL
                     ETVARGLDLTLISIVAEPYALARCLSANAGPDSGAIFIDIGGGTTDIALVRQGGIEET
                     RMFALGGRTFTRRLASSKGLSMKEAEKLKLLYSSGAVKGAEREEVRAILAPEAQLWME
                     SVELLIDELAKNELLPPAIYLVGGGSGLPDLREKLEQFPWKDRLQFSRDPIIETIQPG
                     MVTAINDPSGLLKNAQDITPMALAYQAVELQDQNNALERAMTRVIQRMHI"
     gene            4202..>6391
                     /locus_tag="VGT44_21850"
     CDS             4202..>6391
                     /locus_tag="VGT44_21850"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEV2583520.1"
                     /translation="MSDEQMIYLDPDDDLNRVTERLTAAARQRVTLIVPPQTQMRSNV
                     FWRLLFSRAREMGKDVTVVSADRQVRSVAKIAGFRTDGAPDSSATSKGRSDRGGRTIR
                     SQLESRLGNQQRPQPARPTPRRSGTLPRNQPGSQSNTAQGRPSQQMWRERIPSQADEA
                     FTGPEQHTPDIPAPQIEQDDYDRPFEFRIGASQAIPLSPSTAHQDEREERDPFMPDYE
                     KSRGFLQSWQSGQAAPPATPVQPEPEKTGERTIDFPLSPTQYEPTSSAPSVLPRSTFP
                     ERSLRPPDPFDEFDDQLLAPLPEQRGSAFVHDVEDDVPDIADKPAEVAGAYKIEDLGE
                     DEEETPPASQERQWPDILGDDPEEYEPRRVYGSRPRSSRSGSRRPVRSFYDDEEELAP
                     VDDQPTGPSILPVQSAVPAPMPQQPSRQANTPAAARLTGGAAAPSQRPPRNVTLPQPG
                     APDRVNRTGVTPTVRPALRPTAKPRSSRRGSRALATVLILLILIFLGGVGLFYYGTSA
                     TVTLTVPWKPFTQSKLTLVASTNPQNKAQNTVVSQVLPFIASATGTVPATGTTPQGSS
                     TAKGIATITNNGNVQIDIPTGTTISTSAGPGTLGIQFVTLADALIQSGNSTPIPIQAK
                     SAGVSGNVGAGKIIIIPADSLTAIAAHNHVLTTSLNLSITNSNATTGGGVINVPSATK
                     NDIAALKLKLHQQIQSQVKSWLQAQLESKDQHGAPMPDVQSSATPLSG"
BASE COUNT         1385 a         1970 c         1915 g         1121 t
ORIGIN      
        1 gttgggttta tcatagttta tcataagaac gccgctttta gagagggagg ctcccgtgtc
       61 cgtaacgttg aagctgactg gcaccgaaat tcgccagaag tatctcgatt tcttcgtcag
      121 caaaggccat ttgcgagtgc caagttcgtc gcttgtgccg cgcaacgacc cgaccgtgct
      181 gctaactacc gccgggatgc agcagatgat cccctacttc ctgggacgcg agacgccccc
      241 agcccagcgc ctgacctccg cgcagaaatg ttttcgcacg accgacatcg acaaggttgg
      301 caatgaacgc tcgctgacct tcttcgagat gatgggcaat ttctccgtcg gcgactattt
      361 caagcgcgac gccatcgcct tcgcctggga actgctgaca aagattttca tgatccctgc
      421 cgataaactc tacccgaccg tacatccgca ggatgacgag gccccgcgct ggtggagcga
      481 gattgcaggc taccccgacg agacgattac gcggctggaa gacaactggt ggggacctcc
      541 gggcgcgtcc ggcccatgcg gacccgactc ggagatttac tatgatcgcg gcgtcgagca
      601 tggctgtggg cgggccgact gcgcgcccgg ctgcgactgc gagcgcttcc tggaaatctg
      661 gaacctggtc ttcatgcagt tttaccagga cctcgacggg acacgcacac ccctgccgaa
      721 gaagaacatc gacaccggcc tgggcctgga gcgcgtcagc atggtgctgc aaggcaaaga
      781 atcggtcttc gataccgacc tgttccgtcc catcatcgac aaattcgcct cgcttgccag
      841 aaccacctac ggcagcaacc ccaaagcgga cacctcgctg cgcgttatcg ccgaccacgg
      901 gcgagcgctg gtcttcctgg ccgccgatgg cgtcctgccc agcaacgagg gacgcggcta
      961 catcttccgc cgcatcctga gacgcgcagt gcggcatggc aagctgctcg gcctcgacaa
     1021 gcccttcctg tcggaggccg cggacaccgt catcgacctg atgaaaagcc attatgtcga
     1081 gctgggcttg cagcgcgacc gcatcgtcga gattctcagc ctggaagaga agaaattcag
     1141 ccagacgctc aacaccgggc tggctctgct caccaccctg ctcgacgacc tcaagcagcg
     1201 cggcaagcac gttgttcccg gcgcggatgc ctttaagctg tatgatacgc acggctttcc
     1261 gctggaactg acgcaggaag tcgcggctga gcatggtttc acggtcgata tcaccgcctt
     1321 cgaacaagcc ttgctgcagc agcaggagcg cagccgagcc accgccgctt tcgagcagag
     1381 tacggacgta gacccgctga ccggcctgtt gcagcgcgtc ggcccgaccg aattcaccgg
     1441 ctaccagggc attaccggca gcggcaaggt ggtcgggctg gtcgtggatg gcgtcgaggt
     1501 cgaggagatt agcgcgccgc agtcggccct ggtgatcctt gactccaccc cgttctacgc
     1561 ggaaagcggc gggcagatcg gcgatcgcgg caacatcacc ggctcgatgg gcgtcttcca
     1621 ggtgcaggat acgcagaagc cgctcaaggg gctgatcgtc cactatgggc gcatcgccga
     1681 gggattcatg cgcgtcggcg acagcgtgca ggccagcgtg aacggccagc gccgcgaaga
     1741 caccatacgc aaccacagcg ccacgcacct gctgcacaaa gcgctgcgcg atatgctcgg
     1801 cccgcaggtt gagcagcgcg gctcgctggt tgagccggaa cgcctgcgct tcgacttcgc
     1861 cagcccgcgc gccctgacca gcgctgacct ggcgcgcctc gacgagcaga tcaacgattg
     1921 gattcgcgcc gattatgccg tacagacgcg catcatgccg ctgcaagatg cgctgcaaac
     1981 gggcgcgatg gcgctcttcg gcgaaaagta cgacgagaac gtgcgcgtcg tcacgatggg
     2041 cggcagcacg gagttgtgcg gcggtacgca ctgcgcctcg acggggcaga tcggcgaata
     2101 tatcacgatc caggaaacca gcatctccgc cggtatccgc cgcatcgaag cgctgactgg
     2161 gcgcggcgcg gacgcctatc tgcgccgccg cgtgtccctg gtcgatagta tcagcgcgaa
     2221 gttgcagacg cagcccgatc agcttgaggt gcgcgtagag cagcttgcac aggagcttgc
     2281 caccgcgcgc aagcagctcg cgcagtacca gcgagaggcc gcgcagcagc agaccgaaac
     2341 gctggcgcgt accgccaggg agatcgaagg cgtaccggtg gtcgccaccg tcgccgccgt
     2401 tcccgacgac aaattgctgc gcgagatggg cgatatggtg ctgcgcaagc ttactcgccc
     2461 cggcgtcgtc gtgctggcgg gcaacctcga tgagcgcgtc ggcttgcagg tcagcgtctc
     2521 tcccgagctg gtcaagcgcg gcatccacgc gggcaagata gccggggccg tcggcgagcg
     2581 tctcggcggg cgcggcggcg gcaggccgga gtcagcccag ggtggaggca aaaacaaagc
     2641 cgaactcggt gccgcactcg atatagtggc aaagctggtg aaagaatcac taaaatgacc
     2701 gtaatatctg tagggacagg gggtggcagg gtggagtgaa gagcggctta gctgcatttg
     2761 caagcgtgaa acttttgtgc tataacgtag gtacgtaaca gccgacagga cacgcaaaag
     2821 cagaaacgcg acatgaataa tcccttacag cgcattcgca acttcattac aggcagaact
     2881 acgggaaaca gcgtcgaata cgagcgcgac ggacacgccc actctgctga cgacgaccag
     2941 cggcagttgt tcactgcgct tgatatcggc acggcctacg cgaaggcgct catcatcgag
     3001 gtgcaggacg aaatcgcgat cgtgctggga gcggggcgca acgcccagag ctatcaccat
     3061 atgtctgatg gcatcgttat cgatatccac agtgtcattg agaactgtaa ccaggcctta
     3121 ctgaaggcgg aggaagcggc gggtggcgtt gttgcgcctt ccacgatcat cggcatcgcg
     3181 ggcgagatgg tcaagggcag ttctacaacg gtttcgaggc agcgccagga ccccatgaag
     3241 cttattacgc cggaagagct ggagggcatg attaccagcg cgcagaagaa actgcataaa
     3301 gaggccagcg cgcgtatcgc cgcggaaacc ggctattcaa atgtggaggt gcgcctgacc
     3361 aatgcctcgg tcatttcggc gcgcatcgat gggcagagtg tccataaccc cgtcgacttt
     3421 cgcgggcgca acctggaatt gactattttc agctcgttcg cgccgctgat gcagttgggc
     3481 gcgctggaaa ccgtcgcgcg cggactcgac ctgacgctaa tctcgattgt ggccgagcca
     3541 tacgcgctgg cgcgctgcct gagcgcgaac gccgggccag atagcggcgc aatctttatc
     3601 gacatcggtg gtggcacgac cgatatcgcg ctggtgcgtc agggcggcat agaagagacg
     3661 cgcatgtttg ccctgggcgg gcgcaccttc acacgtcgcc tggcatcgag taagggattg
     3721 tccatgaagg aagccgagaa gctcaagctg ctctacagca gcggagccgt caaaggagcc
     3781 gaacgcgaag aggtgcgcgc gatcctggca ccagaggcac agctatggat ggagagcgtc
     3841 gagttgctga tcgacgagct ggctaaaaac gaactactgc ccccggccat ctacctggtt
     3901 ggcggtggct cagggctgcc cgatctgcgc gaaaaactcg aacaatttcc ctggaaggac
     3961 cgcctgcaat tctcgcgcga ccccatcatc gaaaccattc aaccgggcat ggtgacggcc
     4021 attaacgatc ccagtgggtt gctgaaaaat gcccaggata ttacaccgat ggccctggca
     4081 tatcaggcag tggaattgca ggaccagaat aacgcgctcg aacgcgccat gaccagagtc
     4141 atccagcgca tgcatatcta gcatcctttc aacgttatag accaaggggg agagcagggc
     4201 aatgtctgat gagcagatga tctatctcga tccggatgat gacttaaacc gcgtgacgga
     4261 gcggttaacg gcggcagcca ggcagcgcgt cacactgatt gtgccaccgc agacacagat
     4321 gcgcagcaac gtgttctggc gtctgctgtt ttcacgcgcg cgggagatgg gaaaagatgt
     4381 gacggttgtg agcgctgatc gccaggtgcg ctcggtggca aaaattgccg ggtttcgcac
     4441 cgatggcgct ccagactcct cagcgacgag caagggccgt agcgaccggg gaggtcgcac
     4501 aattcgctct cagctggaaa gtcggcttgg gaaccagcag cgccctcaac ctgcgcgccc
     4561 cactccaaga aggagtggca cgttgccgcg caatcagcct ggctctcaat ctaatactgc
     4621 acaaggacga ccgtcacagc agatgtggcg cgagcgcatt ccttcacaag cagacgaggc
     4681 gtttaccgga ccggagcagc atacgccgga tattccagcg cctcagatag aacaagacga
     4741 ttatgaccgt ccattcgagt ttcgcattgg cgcctcgcaa gctattccgc tcagcccatc
     4801 aacggcgcat caggatgaac gtgaagagcg tgaccccttc atgccggatt atgaaaaatc
     4861 acgcggcttt ctgcaatcct ggcaaagtgg ccaggctgca ccacctgcta ctccagtcca
     4921 acctgagccg gagaaaactg gtgaaaggac catcgatttt ccgctctcac caacacagta
     4981 cgagccaacg agcagcgccc ccagcgtact cccccgtagc acctttcctg agcgcagctt
     5041 gcgacccccg gaccctttcg acgagtttga cgatcagttg cttgcgccac tgccggaaca
     5101 gcgtgggtca gcctttgtgc atgatgtcga ggatgatgtt cccgatattg ccgataaacc
     5161 cgccgaggtc gccggcgcgt ataagattga ggacctgggc gaggatgagg aggagacgcc
     5221 ccccgccagc caggagcgcc agtggcccga tatattaggc gatgatccag aagaatacga
     5281 gccgcggcgg gtgtatggct cgcgtccgcg cagcagtcgc tccggctcgc gcaggccagt
     5341 gcgcagcttc tacgacgatg aagaggagct tgcgcctgta gacgatcaac cgaccggccc
     5401 gtccattcta ccggtgcagt cggctgttcc cgctcccatg ccccagcagc catcgcggca
     5461 ggcgaatacc ccggcggctg cccgcttgac cggcggcgcg gcagcgccat cccagaggcc
     5521 gccacgtaat gtgaccttgc cgcagcccgg cgcgcctgat cgcgtcaaca gaactggcgt
     5581 gaccccaact gtccggcccg cgctgcgtcc caccgcaaag ccccggtcat cgcgccgtgg
     5641 cagccgcgct ctagcgactg tgttgatcct gctgatcctg atcttcctgg gaggcgtggg
     5701 actgttttac tatgggacgt ccgcgaccgt cactttaaca gtgccgtgga aaccattcac
     5761 acagagcaag ctcacgctcg ttgcgtctac taatccacag aataaagcgc agaataccgt
     5821 cgtctcacag gtgttgccct tcatcgccag cgcaaccgga actgtccctg ccaccgggac
     5881 cacaccgcag ggcagctcaa ccgcgaaagg catcgcaacc attaccaaca acgggaacgt
     5941 ccagattgat attcccacag gtaccaccat ctccacctcg gcaggcccgg gaacgcttgg
     6001 catccagttc gttaccctgg cagatgcgct catccagtct ggcaattcta ctccgatacc
     6061 aatccaggcg aagagcgcgg gtgtcagcgg caatgttggc gcgggcaaaa ttatcatcat
     6121 cccggcagat agcctgactg ccattgcggc acacaatcat gttctcacta cgtcgctcaa
     6181 cttgagcata acgaattcga atgccacgac aggcggcggg gtcatcaacg taccctcggc
     6241 gacaaaaaac gatattgccg ccttgaagct gaagctccac cagcaaatcc agtctcaggt
     6301 gaaaagctgg ctgcaagccc aactagagag taaggatcag catggcgctc ccatgccgga
     6361 tgtgcaaagc agcgccactc ccctgtccgg t
//
LOCUS       DASZAK010001241         1936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_281258, whole genome shotgun sequence.
ACCESSION   DASZAK010001241 DASZAK010000000
VERSION     DASZAK010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281258"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..>1936
                     /locus_tag="VGT44_21855"
     CDS             167..>1936
                     /locus_tag="VGT44_21855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583521.1"
                     /translation="MQSIARTMTVVKVVGWIAAAMIVIVVFHLGGIVALRVGQFSPLT
                     GAFIGGTLTLACVLVPMRKREQIEPWIKHERVAWALIGCGVLMWGFGESFWRYYISIG
                     QQPFPSTADIGYSSFPPLVFLGLLLQPSPGTGNRRLLLVLDSLIAMGSILSIAWYLLL
                     GSLAQAPGEANLAKFLGLYYPVADMALLSCVVFLVMRGQGRTYLATARRAGLLVIALG
                     LCFFVFSDFLFNVQNNAGTYVEATWIDLGWPLGLMTIGIAACLRRFLPVTSIEVIEER
                     MDRLAEQDGFGLSRYVPYILLTLLFIALTANVLSTDSGQIAIRPVLLFATMGVVALVV
                     ARQLLTLWENIRLSARQAEALEDLERANHRIEEQAHHIADHSEQLERGIAHLKHVQAQ
                     LSNGNLQARAELTSGVLLPLAGSLNLMAERLTRLGQASVYTRRLLHSLGELSVALERA
                     ASGANFTIPASCHEFVEIDRLLVALRSNNRHLMASYTPEPPSTPLPRASQSLPPVPSM
                     PPMPVTPSASRPGTEPLLVRPAGRMVFGSSASRPFPASGSLRASAPAQSTPADGMRPL
                     PSSGSLPPLQSIRRPPSLEAFGGD"
BASE COUNT          345 a          585 c          575 g          431 t
ORIGIN      
        1 tcagggtatt tacttgctac caggtgtcca tattgagaga cgcgctgaac ccgtatttgt
       61 aaaaaatcac ggatattcct gtgtagcctg ctgggatatg acggaaatgt gacatgggta
      121 tactgcctct ggttgtataa gcaatttagc agaatgggag gtactcatgc aatcgatagc
      181 caggacaatg acggttgtga aggtcgtcgg gtggattgcg gcggccatga tcgtcatcgt
      241 ggtattccac ctggggggca ttgttgcgct gcgagtgggg cagttctcac cgctcaccgg
      301 cgcattcatt ggcggaacgc tgacgctggc ctgcgtgctg gtgccgatgc gtaagagaga
      361 acagatcgaa ccctggatca agcacgaacg ggtcgcctgg gcattgatag ggtgtggagt
      421 gcttatgtgg ggcttcggcg aaagcttctg gcgctactat atttctatcg ggcagcagcc
      481 ctttccgtca acagccgaca tcggctactc cagctttcca ccgcttgtct tcctgggcct
      541 gctgctccaa ccatcgccgg gaacaggcaa tcgccgactc ttgctggtgc tggatagcct
      601 gatcgccatg ggatcgatcc tgtctatcgc ctggtatttg ttgctcggtt cgctggcgca
      661 agcacccggc gaggctaacc tggcgaaatt cctggggcta tattatccag tcgcggacat
      721 ggctctgttg agctgcgtcg tcttcctggt gatgcgcggg caaggtcgaa cctacctggc
      781 gactgcgcgg cgcgccggct tactggtaat cgccctgggt ctctgcttct tcgtcttctc
      841 agatttcctt ttcaacgttc agaacaatgc cgggacgtat gtcgaggcga cctggatcga
      901 cctgggctgg ccgctgggtt taatgaccat aggcatagca gcctgcctgc ggcgtttcct
      961 gccggtcaca tcaatagaag tgatcgagga gcgtatggac cgcctggcgg agcaagacgg
     1021 cttcggctta tcgcgttacg ttccctatat cttgctcact ctgctcttca ttgcgctgac
     1081 cgccaatgtg ttatcgacgg acagcggtca gatcgctatt cgaccggtgc tgctcttcgc
     1141 cacgatgggc gtggtggcgt tggtggttgc tcgccaactg ctgacgctat gggagaatat
     1201 ccggctatcg gcgcggcagg ccgaagcgct ggaagacctg gaacgcgcga accaccgcat
     1261 cgaagagcaa gcgcatcata tcgccgacca cagcgagcaa ctggagcgcg gcatcgctca
     1321 tctgaagcat gtgcaggcgc agctgtccaa cggcaatcta caggccagag ccgagctgac
     1381 gagcggcgta ctgctgcccc tggcgggtag tctgaatctg atggccgagc gactgacgcg
     1441 cctgggtcag gccagtgtct atacccggcg tctgctgcac tccctgggcg aactcagtgt
     1501 cgccctcgaa cgagcggcct ccggcgcgaa tttcaccatc cctgcttcgt gccacgagtt
     1561 tgtcgagatc gaccgcctgc tcgtagctct gcgctcgaat aacaggcatc tcatggcctc
     1621 ctacacgcct gagccgccat caacgccgct cccacgcgcg agccaatctt tgccgcctgt
     1681 gccttcgatg ccaccaatgc cggtcacacc ctcggcgagt cggccaggca ccgagccgct
     1741 cttagtgcga cccgccgggc gaatggtctt cggttcgtcg gcatcgcgtc cgttcccggc
     1801 gtccggctcc ttgcgcgcct cggctcccgc tcaatccacg cccgccgacg gtatgcgccc
     1861 cctgccatca tcaggatcgc tgccgccctt acaaagcatt cgccgcccgc cgtccttgga
     1921 ggcatttggg ggagac
//
LOCUS       DASZAK010001242         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_136637, whole genome shotgun sequence.
ACCESSION   DASZAK010001242 DASZAK010000000
VERSION     DASZAK010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136637"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="VGT44_21860"
     CDS             <1..724
                     /locus_tag="VGT44_21860"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEV2583522.1"
                     /translation="PPHPLILQSGEVHAWRAALEQPDAVCARLQATLAPDELAKAVRF
                     HFARDRQHYIIARGILRTLLYGYTKVHPLSMRFAYNAYGKPSLDMAGVEQDIRFNLAH
                     SRDLALYAFTLSHEVGIDVEYMKTDIDAEQVARHSFSPEEQAALRALPPGERLQGFYN
                     GWTRKEAYIKARGMGVSLALDSFDVALKPGDPAALLRSREDAREPMRWKLMELEPGEG
                     YAGALAVEGQHWRLHYFQWHGD"
     gene            complement(859..1455)
                     /locus_tag="VGT44_21865"
     CDS             complement(859..1455)
                     /locus_tag="VGT44_21865"
                     /inference="COORDINATES: protein motif:HMM:TIGR04342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EXLDI protein"
                     /protein_id="HEV2583523.1"
                     /translation="MPNRTIYVADADLPIFEKAQKLTGDNLSAAIARALRSFVEQEEA
                     RQGGYEDVSIKVGKGRPFLQQHFRGRLLARRHIRISNDARILTLSVYQTAKGRFAVYS
                     KNQPNWNGWSQYWAKQSKQQRQSRPGNQDWDWLQNWDWSQNSNWSQNWDWSTYQDDEE
                     YRLDVFESLDQLKENVPEELFDAANRYLNGEDAEFLDI"
     gene            1670..2542
                     /gene="galU"
                     /locus_tag="VGT44_21870"
     CDS             1670..2542
                     /gene="galU"
                     /locus_tag="VGT44_21870"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV2583524.1"
                     /translation="MRIRKAVLPVAGLGTRVLPASKVIPKEMLPLVDKPTLQYIVEEA
                     VDAGVEEIIFVTSRSKRSIEDHFDSFPELEAVLERKGKRKELAELRALQTMATYTSVR
                     QPEPLGLGHAVLCAKELVGDEPFIVMLGDILIPPETPCLPRLIEVYEQYGGPVLSLVP
                     LPPEVVPTYGIASIEQLDGDAVKITHLVEKPALEEAPSNLGVYGRYVLTPDIFALLER
                     TPPGKGGEIQVTDAIEMQVRAGQCYGVSYPGERYDTGTPLGLLTTSIAFALKRPDLAD
                     DLRAYMREVLGVSL"
BASE COUNT          577 a          767 c          770 g          572 t
ORIGIN      
        1 accgccgcac ccgctcatcc ttcaatcagg cgaagttcat gcctggcgcg ctgcactgga
       61 acagcccgat gcggtatgtg cgcgcctgca agccacgctt gcaccggatg aactggcgaa
      121 agccgtgcgt tttcactttg ccagggaccg ccaacactat atcatcgcgc gcggcatctt
      181 acgaacccta ctctatggct atacgaaggt tcacccgctt tcaatgcgct tcgcctataa
      241 tgcctatggc aaaccttccc tggacatggc cggtgttgaa caagatatcc gcttcaatct
      301 cgcgcattcc cgcgacctcg ccctctatgc cttcacgctc agccatgaag tgggcattga
      361 cgtggaatat atgaaaaccg atatcgacgc ggagcaggtg gcgcggcaca gcttttcgcc
      421 agaagaacag gctgccctgc gcgcgctgcc gccgggagag cgattgcagg gattttacaa
      481 cggctggacg cgcaaagagg cgtatatcaa agctcgtggg atgggcgttt cgctcgcgct
      541 cgatagtttc gatgtagcgc tgaaaccggg agatccagcc gcgctgttac gtagccggga
      601 ggacgcgcgg gaaccaatgc gatggaagct aatggaattg gagccgggtg agggatacgc
      661 aggcgcgctt gccgtcgaag ggcagcattg gcgcctgcat tactttcagt ggcatggcga
      721 ctaatcttcc gggtggaaag agtgcatggc gctgactaca tcttcttcga gtgggcggag
      781 tgctgtttag cgcatccctt ccattcgatg gtcatcgcgc tgcgagacgc gaagtacatc
      841 ctcgaattcg acgataagct aaatgtccag aaattccgcg tcttcaccgt tgagatagcg
      901 gttggccgcg tcgaacaact cttcagggac gttctctttg agttgatcga gcgactcgaa
      961 gacatccagg cggtattcct cgtcatcttg ataggtggac caatcccagt tctgggacca
     1021 gttcgagttt tgggaccagt cccagttctg caaccaatcc caatcctggt ttccagggcg
     1081 agattggcgc tgctgtttcg actgcttggc ccagtattgc gaccagccat tccagttcgg
     1141 ctggttcttc gagtacaccg cgaagcggcc tttggctgtc tggtagacgc tcaacgtcag
     1201 aatgcgcgcg tcgttgctga tgcggatatg acggcgggcg agcaaccgcc cgcgaaaatg
     1261 ctgctgcaag aaaggtcgcc ctttcccaac tttgatgctc acatcttcat atccgccctg
     1321 ccgcgcctct tcctgttcga caaagctgcg caaggcacgc gcaatggcgg cggagagatt
     1381 gtcccccgtc aatttctgcg ccttctcgaa gatcggcaaa tcggcgtcag caacataaat
     1441 ggttcggttc ggcatagcaa acgctccttg ttccatcatt tccggtactc cttgtgctgg
     1501 gtatggcaac tgcgatacgc ccggtagaat attgtctata ttgtaatata atatatgtat
     1561 acgtatatgt caatagcaag aatggccagg gcgatggcaa gcatcgccct ggccattctt
     1621 gctataatgg aggtagcgaa aacctgcatt attgtgttga ggaggattca tgcgtatacg
     1681 aaaagcggtg ctgccagtgg cgggacttgg gacgcgcgta ctgccggcca gcaaggtgat
     1741 cccaaaagaa atgctgccac ttgtcgataa gccgacattg caatacatcg ttgaggaggc
     1801 ggttgatgcc ggtgttgagg agatcatttt cgtcaccagc cgcagcaaac gcagtatcga
     1861 agatcacttt gactcgtttc ccgaactaga agccgtcctg gagcgcaagg ggaaacgcaa
     1921 agaactggcg gaacttcgcg ccctgcaaac gatggcgacc tacacctcgg tgcgccagcc
     1981 agagccgtta gggctgggtc atgctgtgct gtgcgccaaa gaactggtgg gggatgaacc
     2041 gttcatcgtc atgctcggcg atatcctgat cccgccggag acgccgtgtc tgccgcgcct
     2101 gattgaggtc tacgagcaat acggcggtcc cgttctctcg ctcgttcccc tgccgccgga
     2161 ggtcgtcccg acctacggca tcgccagcat cgagcaactg gacggcgacg cggtgaagat
     2221 tacccatctg gtagaaaagc ccgccctgga agaagcgcct tctaacctgg gcgtctatgg
     2281 ccgctatgtg ctgacgcccg acatcttcgc tctgttggaa cgcacaccgc caggtaaagg
     2341 cggggaaatc caggtgacgg acgccatcga aatgcaggtc cgggctggcc aatgctacgg
     2401 cgtgagctac cccggtgagc gctacgatac cggcacgccg ctcggcctgc tgaccacctc
     2461 gattgccttc gccctgaaac gccccgacct cgccgacgat ctgcgcgcct atatgcgaga
     2521 ggtgttaggg gtatctttgt gacgcacaac acacggcgag cgcacaacac atggcgaccg
     2581 cagaagacat ggcgaccgca gaagacatgg cgaccgcaag ggtcgccact acgataacgg
     2641 accttcgcag gccgattcac atgcgttatc gtagtggcga cccttg
//
LOCUS       DASZAK010001243         1640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_136658, whole genome shotgun sequence.
ACCESSION   DASZAK010001243 DASZAK010000000
VERSION     DASZAK010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136658"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VGT44_21875"
     CDS             <1..715
                     /locus_tag="VGT44_21875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEV2583525.1"
                     /translation="LVGGGAQAEYVVAHEGLLVAIPDNLDFEQAAGVPEAFMTAHDAL
                     FTQAGLQMGERVLVHAVGSGVGTAAVQLAGAAGATTYGTTRTPAKLEAAKVLGLNVGL
                     SDQGFAAEVARLTNNEGVHVVIDFVGAPYLEQNLQALASWGRIVFLSTMGGVHADVNL
                     GMLMQKRISMRGVTLRTRTLEEKLAVTRRFATGVLPLLASGKVRPVIEQVYPLEQIAE
                     VHKVMGENRNFGKLVVRVD"
     gene            complement(712..1458)
                     /locus_tag="VGT44_21880"
     CDS             complement(712..1458)
                     /locus_tag="VGT44_21880"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEV2583526.1"
                     /translation="MHLGVFFTPSSIVTTDSSANDIYIVIDVIRATTSIAVMFDRGAE
                     RVLVAGSIEQAREAAQKTPGRLLCGERNVQKIPGFDYGNSPTEFALLDLSGRELIATT
                     TNGTRAFYACPAQATRLAGSMYNAGVVAARALQLAQAQGSATINLVCSGELNYFALDD
                     TVCAGYLALEIQQLSPDIQLWESALAAITLYEAYKPPKVLEVANSAQTVFQGGLVDDP
                     PFCMRINVSTSVPVVVGREAATGLLVLEKG"
BASE COUNT          323 a          415 c          550 g          352 t
ORIGIN      
        1 gctggtcggc ggcggcgcac aggccgagta tgtcgttgca cacgaagggc tgctggtcgc
       61 tattcccgac aatctcgatt ttgagcaggc cgccggtgtg ccggaggctt tcatgacagc
      121 gcatgatgcg ttgttcaccc aggcaggttt gcaaatgggc gagcgtgtgc ttgtgcatgc
      181 agttggcagc ggtgttggca cggcagccgt gcaactggcg ggtgcggcgg gcgcgactac
      241 ctatggaact acgcgcacac ccgccaagtt ggaagcagcg aaggtattag ggctgaatgt
      301 tggtctctcc gatcagggct ttgctgccga ggtcgcgcgc ttgactaata atgagggcgt
      361 acacgtggtg attgattttg ttggcgcgcc ctacctggaa cagaacctgc aggcgctggc
      421 ttcctggggc cgcatcgttt tcctctctac gatgggcggc gtacacgcag acgtgaacct
      481 gggtatgttg atgcagaaac gcatctccat gcggggcgtg acgctgcgca ctcgcaccct
      541 cgaagagaag ctggccgtga cgcgccgttt cgctactggc gtgttgccct tgctggcaag
      601 cgggaaggtc aggccggtga tcgagcaggt gtacccgctg gagcagatcg cggaggtaca
      661 caaggtgatg ggggagaatc ggaattttgg gaagctggtg gtgagggtgg attaaccttt
      721 ctccagtacc agcaacccgg tcgctgcttc tctccccacg acgaccggca cgcttgtact
      781 cacgtttatg cgcatacaga atggcgggtc gtctaccagg ccgccctgga agacggtctg
      841 ggcggagtta gcaacctcaa gcacctttgg cggtttgtag gcttcgtaga gggtgatggc
      901 ggccagggcg ctttcccata gttggatatc aggggagagc tgttgaatct ccaatgcgag
      961 atagcccgcg cagacggtgt cgtccagggc gaagtagttg agttcgccgg agcagacgag
     1021 gttgattgtg gcgctgcctt gggcctgcgc cagttgcagg gcgcgagcgg caacgacccc
     1081 ggcgttgtac atgctgcccg cgagacgagt ggcttgcgcg gggcaggcgt agaaggcgcg
     1141 ggtgccgttg gtggtggtgg caatcagttc gcgcccgctc agatcgagca gcgcgaactc
     1201 ggtgggtgag ttgccgtagt cgaagccggg gattttctgg acgttgcgct caccgcagag
     1261 caggcgaccg ggggtctttt gcgccgcttc gcgcgcttgc tcgatggaac ctgccaccag
     1321 cacacgctcc gcgccgcggt cgaacatgac ggcgatagag gtggtggcgc gaatgacatc
     1381 gatgacgatg tagatgtcat tcgcgcttga gtctgtagtg acgattgagg aaggagtaaa
     1441 aaagacgcct aaatgcaaga ttacctcaaa ttcacatgtc gtagcaccct attcaggagg
     1501 atatacctgt tcgcaatcaa agggatgaat ggcgcaacaa ggaatatctg atactatggt
     1561 atcacgaaag ccagtgcgtt tcatcaagtt ggcatgagag ttgaaggaat gtttttcgct
     1621 tatggcactt cactcaactt
//
LOCUS       DASZAK010001244         4111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_281304, whole genome shotgun sequence.
ACCESSION   DASZAK010001244 DASZAK010000000
VERSION     DASZAK010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281304"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2056)
                     /locus_tag="VGT44_21885"
     CDS             complement(<1..2056)
                     /locus_tag="VGT44_21885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2583527.1"
                     /translation="MSTHPRRLGKYELQERLGQGGMAEVWKALDTQLRRYVAIKLLHA
                     NLKEDPHFIARFEREAQLIASLHYPNIVQIHDFQAASSEQEGTFAYMVMDYIEGQTLA
                     DYIHQTSGRGNFPSPTEIVQLFASIGLAVDYAHQQGMIHRDLKPANILLDQRQTTINP
                     MGEPILTDFGLAKLLGSSSATLTATQLGTPLYIAPEQASGFAGNERSDIYSLGIILYE
                     MVTGVLPFQGDSPTAVMAQHLKATPPSPSLINPNIPPALEFVVMRCLAKDPAARFPSA
                     SSLAAAIAESLKVPVPESLGKPTYPVDAEYMPTYLTPSPSSLTPGATPSSPALPIVKT
                     STPQPPVIATAGSSPGLSSSGGQVTPPLSMGSNPGISTPPYASTPGTPFPPSQSFSTA
                     TFSTQTPPGALSGRNPGSGSFAPAQSPAAAPAPLLDTTPTSRPPRQRRWLLFGLIALI
                     IVLLGSSLGAYFLSLRSGGPVPPAPNPIVGHAFYVSSGQLSDGAQGIADELQVDLQNV
                     APPPAGKSYYLWLLGDKDTTPVADLLVPPPIHPPILVTNNLPVQSNGNVHYLYQGDAQ
                     HNNLLSETSRLLITLENAGSTPASPSTDRSTWIYYAELPQALIPKDASGLSLRGLDHI
                     RHLYYNENHLPVLGLYGGLDIWVFRNTESILEAATSARDDFGLPGSYPTMHSLFTTIL
                     DYL"
     gene            complement(2072..>4111)
                     /locus_tag="VGT44_21890"
     CDS             complement(2072..>4111)
                     /locus_tag="VGT44_21890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2583528.1"
                     /translation="AEIVNLLAPIASALDFAHQRNVIHGALRPAAILLGKQGETSPGE
                     PKLTDFGLNHMQNPLALPLDDVQYVSPEIAQGLASTQRSDLYSLGVILYEICTGTVPF
                     HGDTSSDILMQHIHGTPPSPVLINPYIPPGLTAVIMRSLAREQAARYPSAIALVAAVA
                     KAANVNMPESISRALPSVSSINPPSFSGISGPLDTMNSPTYLSQQSQQLPPKALPVPP
                     VVASRDTPALPRNPVISPSTPVLPLMQTGERYAPTAQIAQPSQSVSATTPVLAPAPLK
                     QRRRPTWLYIALAAAVLLVVVGGSALGLYLYNSRTVPPSSPPIVGHAFFMSSGLLGGI
                     SSNQGITDELEISLQNIPNPQPGKSYYAWLLNDTQTSLPAVPLGPLLVTHGQVTKLYV
                     DQLHNNLLTNYSRFLITEEGTSPPPVTPSLDNTAWVYSAAFPTTPNPADTVNHFSVLD
                     HLRHLLSQDPKLKTAGLGGGLDIWLFRNVTKVVEQAGSARDAQGQCTPAPNNASCGDI
                     HRAVVRVLDYLDGSTYVNTEVPSNTPLLINQTIARVALLEFDPVHQQPPGYLDHIGTH
                     LRELANAPGVTTGQHNLAIQIGQAINNVQGWLQAVRADAVKLVQMNNSQLSQPAALTL
                     LNDLLTQAKYALVGFLDPNTNTVRDGVAQIHDDIQGLATFNVTPCTTTNGKNSCA"
BASE COUNT          844 a         1059 c         1332 g          876 t
ORIGIN      
        1 ccagataatc caggatggta gtgaacagcg agtgcatcgt tggataactt cctggtaagc
       61 cgaagtcgtc tcgcgcgctg gtagccgcct ccagaatact ttctgtattc ctaaagaccc
      121 agatgtccag tccgccatac agtcctaaca cagggaggtg attctcgtta tagtacagat
      181 gccgaatatg gtccagcccc cttaaactca aaccagaggc gtctttcgga atgagcgcct
      241 ggggcagttc cgcgtagtag atccaggtcg agcggtcggt cgatgggctg gcaggggtgc
      301 ttcctgcatt ctccaaagta atgagcaagc gactggtttc tgagagtaga ttgttatgct
      361 gtgcatcacc ctggtagagg taatggacgt tcccattcga ctgcacgggc aagttgttcg
      421 tcaccaggat gggcgggtgt atgggcggtg gtacgaggag gtctgcgact ggcgtcgtgt
      481 ctttgtcacc cagcagccag aggtaatagc ttttccctgc cggaggaggc gcgacgtttt
      541 gcagatcgac ctgcaactcg tcggcgatgc cctgtgcgcc atcactcagt tgtccgctgc
      601 tgacatagaa ggcatgtccc acaatcggat ttggcgcggg gggtacggga cctccagagc
      661 gtaaggacag gaagtaagcc cccagcgagc tgcccagcag gacgatgatg agcgcgatca
      721 acccgaagag cagccatctg cgctgccggg gaggtcgcga cgtcggggtt gtatccagca
      781 gaggcgcagg tgccgccgct ggtgattggg ctggcgcgaa tgatccgctg ccagggtttc
      841 taccagatag cgcccccggg ggcgtctgtg tgctaaaggt tgcggtagaa aacgattgcg
      901 atggcggaaa aggggtaccc ggcgttgacg catacggagg cgtggaaatt ccagggttac
      961 tgcccatcga gagcgggggt gttacctgtc cgccgcttga ggagagacca ggcgatgaac
     1021 cagctgtggc aatcaccgga ggctgcggcg tagaggtttt cacaatgggc agcgcgggtg
     1081 aagagggcgt cgcccctggc gtgagactgg atggactagg ggtgagatag gttggcatat
     1141 attcagcgtc gaccggatag gtcggtttgc ccaggctttc cggcaccggc acttttagcg
     1201 attcggcgat ggctgccgcc agcgacgagg cgctggggaa gcgcgccgcc gggtcttttg
     1261 ccaggcagcg catgacgaca aattccagcg caggcggaat gttcggattg atgagcgaag
     1321 gagagggcgg ggtcgccttg agatgctgcg ccatcaccgc tgttgggctg tctccctgaa
     1381 agggcaggac gccagtaacc atttcataca ggatgatgcc cagcgagtag atatcgctgc
     1441 gttcgttgcc agcaaagccg ctcgcctgtt cgggcgcgat gtacaggggc gtcccaagct
     1501 gcgtcgcggt aagggtcgcc gaggacgagc cgagcaactt cgccagccca aagtcggtga
     1561 gaatgggttc gcccatgggg ttgatggtgg tctgccgctg gtcgagcagg atattggcgg
     1621 gtttgaggtc gcggtgaatc atgccctgct ggtgagcata atcgacggcc aggccgattg
     1681 aggcaaagag ttgcacgatc tcggttggag agggaaagtt gccacggccc gaggtctggt
     1741 gaatgtagtc ggccagcgtc tggccttcga tgtaatccat gaccatatag gcaaacgtcc
     1801 cttcttgctc ggaagaagcg gcctgaaagt cgtggatttg cacaatgttg ggatagtgca
     1861 gcgaggcaat caactgcgct tcacgctcga accgggcgat aaaatgaggg tcttccttca
     1921 gattggcgtg cagcagcttg atggcgacgt agcgccgcag ttgcgtatca agcgctttcc
     1981 agacctccgc catgcccccc tgcccgagtc gctcctggag ttcatattta ccgaggcgtc
     2041 gtggatgggt actcattgct catctcctca tctatgcgca ggaattcttg ccattcgtgg
     2101 tggtacaggg cgtcacattg aaggttgcca acccctgaat gtcgtcgtga atttgtgcca
     2161 ctccgtcccg cacggtattg gtgttggggt cgagaaagcc gaccagggcg tacttggcct
     2221 gggtgagcag gtcattgagg agggtcaggg ccgccggctg ggacaactga ctattgttca
     2281 tctgcaccag cttcaccgcg tcagcacgca cagcttgtag ccagccctgc acattgttga
     2341 tggcctgccc aatctgaatc gcaagattat gctgacccgt ggtcacgccg ggagcattgg
     2401 ccagttcgcg caggtgggtt ccaatatggt ccaggtagcc gggaggctgt tgatgcaccg
     2461 ggtcgaactc gagcaaagca acgcgcgcaa tagtctgatt aatcaacaga ggggtattgg
     2521 atggaacctc tgtgttgaca taggtactgc catccaggta gtccagaaca cgcaccactg
     2581 cccggtggat gtcgccgcac gaagcattat ttggagcagg cgtgcattgg ccctgcgcgt
     2641 ctctggcgct gcccgcctgt tctacgacct tcgtaacatt cctgaacagc cagatgtcca
     2701 gccctcctcc caacccggcg gtcttgagct tagggtcctg ggagaggaga tggcgaagat
     2761 ggtcgaggac actgaaatga ttgacggtat ccgccggatt gggcgtcgtt gggaaggcag
     2821 cggaatagac ccacgctgtg ttgtcgagag agggggtaac aggaggggga ctcgtgcctt
     2881 cctcggtgat gagaaaacga ctatagttgg tgagcaggtt gttatgaagc tgatcgacat
     2941 acaatttcgt gacctgccca tgagtgacga gtagcggacc aagcgggaca gctggcaaac
     3001 tcgtctgggt atcgttcagc agccaggcat aatagctttt gccgggctgc ggattgggaa
     3061 tgttctgcaa gctgatctct agctcatcgg tgatgccctg gttgctgctt ataccaccca
     3121 acaggccgct gctcataaag aaggcatgtc ccacgattgg aggggaactc ggaggcacag
     3181 ttcgcgagtt gtagagatag agtcctaaag ctgagccgcc gaccaccacc agcagcacgg
     3241 ctgccgccag cgcaatatag agccacgttg gcctcctcct ttgtttgagt ggcgcaggcg
     3301 caagcacagg cgtcgtcgcc gagacagatt gagatggctg cgctatttga gcggttggcg
     3361 cgtacctctc tcctgtctgc atcaaaggca agacgggtgt ggaaggcgag atgacgggat
     3421 tgcgcggcag agcaggcgtg tccctgctgg ctacaaccgg tggaacgggg agcgcttttg
     3481 gcggcaattg ttgtgactgc tggctgagat acgtcgggct attcatcgta tccaggggac
     3541 cgctgattcc actgaacgat ggcggattaa tgctgctcac cgatgggagc gcgcgactga
     3601 tgctttccgg catgttcacg tttgccgcct tagctactgc tgcaaccagc gctatcgcgc
     3661 tggggtaacg cgcggcctgc tccctggcga ggctgcgcat gatgaccgca gtcagcccag
     3721 gcggtatata gggattgatg agtactggcg agggaggcgt accatgaata tgttgcatca
     3781 aaatatcgct ggatgtatcg ccgtggaaag gcacggtgcc cgtacatatt tcgtagagta
     3841 tcacgccaag cgaatacaga tcgctgcgct gcgtactggc aagaccctgg gcaatctctg
     3901 gtgagacata ttgaacgtcg tcgagtggga gcgcgagcgg attctgcatg tgattcaagc
     3961 caaaatcggt gagtttcggc tctcccggtg atgtttcccc ctgcttgccg agcagaatgg
     4021 ctgccggtct aagcgcgcca tgtatcacat tgcgctggtg ggcgaagtca agtgcggagg
     4081 cgattggcgc cagcaggttc acaatctcgg c
//
LOCUS       DASZAK010001245         1763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_66503, whole genome shotgun sequence.
ACCESSION   DASZAK010001245 DASZAK010000000
VERSION     DASZAK010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66503"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VGT44_21895"
     CDS             <1..208
                     /locus_tag="VGT44_21895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV2583529.1"
                     /translation="GPLKGILAFTDEPLVSSDFRGDPHSAIIDGLSTMAIGNMVKVIA
                     WYDNEWGYSSRVADLTNFIAQKGF"
     gene            227..1426
                     /locus_tag="VGT44_21900"
     CDS             227..1426
                     /locus_tag="VGT44_21900"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV2583530.1"
                     /translation="MNKKTVRDIDVQRKRVLERVDFNVPLDAERHITDDTRIRAALPT
                     ILYLLDHGAAVTLMSHLGRPDGKVVEKLSLAPVAERLSELLGRPVAMAADCIGPQVEE
                     QARSLQAGQVLLLENLRFHKEEEKNDPAFAQQLASLGEVYVNDAFGTAHRAHASTEGV
                     TRYLPAVAGFLMEKELNYLGSALESPRRPFASIVGGAKVSDKIAVLERLISMVDSLLI
                     GGGMANTFLKAEGYEIGDSLFEESKVEMARDLLAKARQRGLNLLLPSDVVIADRFAAD
                     AAHKVVAAGSVPQGWRILDIGPRTVEAFRIALADAKTVVFNGTLGVAEMTAFARGTNA
                     IIDILAQCTQAGATTIIGGGDSAAAVEAAGAAEKMSHVSTGGGASLEFLEGRVLPGVA
                     ALQDKDA"
     gene            1423..>1763
                     /locus_tag="VGT44_21905"
     CDS             1423..>1763
                     /locus_tag="VGT44_21905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV2583531.1"
                     /translation="MTAQQSTQRTAIIAGNWKMNYGPRHAAEFAHEIVPQLHSLMQTN
                     EHIMSILCPPAISLSAVHGVLHAHPHERIELGAQNMFFEEKGAFTGETAPSMVRELCT
                     TVILGHSERRT"
BASE COUNT          369 a          551 c          523 g          320 t
ORIGIN      
        1 cgggccgctc aaaggtattc tcgcgtttac cgacgagccg ctggtctcaa gcgatttccg
       61 gggggacccg cactccgcca tcattgatgg gctttccaca atggctattg gaaacatggt
      121 caaggtcatc gcctggtacg acaacgaatg gggctactcg tcgcgcgtcg ccgatctgac
      181 caacttcatt gctcagaagg ggttttaacc cttacaggat atcgtcatga ataagaaaac
      241 agtacgcgat atcgatgtgc agcggaagcg cgtcctggag cgcgtcgatt ttaacgtgcc
      301 cctggacgca gagcggcaca tcaccgatga cactcgcatt cgagctgccc tgcccaccat
      361 tctgtatctg ctcgatcatg gagccgctgt gaccctgatg tctcacctgg gtcggcctga
      421 tggcaaggtt gtcgaaaagt tgagcctcgc gcccgttgcc gagcggctga gcgaactgct
      481 cggcaggccg gtcgcgatgg cggcggactg tatcggcccg caggttgaag agcaggcgcg
      541 ttcgctgcaa gcgggacagg tcctgttgct ggaaaacctg cgcttccaca aggaagagga
      601 aaagaacgat ccagcctttg cgcagcagct cgcctcgctc ggcgaagtct acgtcaacga
      661 tgccttcggt acagcgcatc gcgcccatgc ctcaaccgaa ggtgtaacac gctacctgcc
      721 cgccgtggcg ggcttcttga tggaaaagga actgaactat ctcggctctg ccctggaaag
      781 cccgcgtcgt cctttcgcct caatcgtagg cggcgcgaag gtctccgata aaatagccgt
      841 gctggaacgc ctcattagca tggtcgatag cctgctgatc ggcggcggca tggcgaatac
      901 gtttctgaag gcagaaggct acgaaatcgg cgattcgctc ttcgaagaga gcaaagtcga
      961 gatggcgcgt gatctgctgg cgaaggcgcg acagcgcggc ctcaacttgc tcctgccatc
     1021 cgacgtagtg atcgccgacc gcttcgccgc cgacgccgcg cataaggtgg ttgccgccgg
     1081 tagcgttccg cagggctggc gcatcctcga catcggcccg cgcacggttg aggcattccg
     1141 aatagcgctg gccgacgcga agaccgtcgt cttcaatggc acgctcggcg tggccgaaat
     1201 gaccgccttc gccagaggca ccaacgccat catcgacatc ctggcgcaat gcacgcaggc
     1261 gggcgcgacc accatcatcg gcggtggcga ctcggcggcg gcggtcgagg cggcaggcgc
     1321 ggcggagaag atgtcgcacg tctcgaccgg cgggggcgca tccctcgagt tcctcgaagg
     1381 ccgcgtgtta ccgggtgtgg cagcactaca ggacaaagac gcatgacagc acaacagagt
     1441 acacagcgca ccgccatcat cgcgggtaac tggaaaatga actacggccc cagacacgcg
     1501 gctgagttcg cgcacgaaat cgttcctcaa ctgcatagcc tcatgcagac caacgaacac
     1561 atcatgtcga tcctctgccc gcccgctatt tcgctctcag ccgtacacgg cgtcctacat
     1621 gcgcaccctc acgagcgcat cgaactcggc gcgcaaaaca tgttcttcga ggagaagggc
     1681 gcgttcaccg gcgaaacagc gccctcaatg gtacgcgaac tctgcacgac cgtcatcctg
     1741 ggtcactccg aacgtcgcac cat
//
LOCUS       DASZAK010001246         3655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_637412, whole genome shotgun sequence.
ACCESSION   DASZAK010001246 DASZAK010000000
VERSION     DASZAK010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3655
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_637412"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..1062
                     /locus_tag="VGT44_21910"
     CDS             148..1062
                     /locus_tag="VGT44_21910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEV2583532.1"
                     /translation="MEPAHFSGQTLVLLNPAANRGNMHRHRAAVRQRAEQAGAEYIET
                     TLQGEAKERAQQAAAEDRPIIIVGGDGSVHEAVNGILASGRRVPLGIVAAGSGNDFAW
                     NTLKLPHDPQAAVERAFNGEPVEVDAGVVNERYFANSFSVGIDADIAVAAGKMKRYPF
                     MSGARLYYTTTIKQLLFGYGRCPWLAFELDGGDVIQAGERRYILMAVTNGPTYGAGFR
                     INPRADHADGLFDICAISHTPLARALKLLPVVQKGEHESLPEVTFYHARTVSIKSRLP
                     VNIQMDGETTRATSFHAECLPGALLVRV"
     gene            1070..1702
                     /locus_tag="VGT44_21915"
     CDS             1070..1702
                     /locus_tag="VGT44_21915"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEV2583533.1"
                     /translation="MLYNVLRALFWGFTHLVCRYRVVGLERVPSSGSLLIVANHLSFY
                     DPMLLGVILPRRAWIFTKAEIFRWPIAGAACKYTGQIPVRRGESDRSALEKALVYLQE
                     GKALMLFPEGTVERQEQMIPARAGAAMLAARSGATILPIAHVGTRRILRTPRSWFPRV
                     TITIGEPFTPTIPTDVPRKVGLQHVTDEIMQRIATMLPPENRGVYAENLL"
     gene            complement(1750..2421)
                     /gene="nth"
                     /locus_tag="VGT44_21920"
     CDS             complement(1750..2421)
                     /gene="nth"
                     /locus_tag="VGT44_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV2583534.1"
                     /translation="MELDTPTYAGPTPGSPEQVTAIMAELRRLYPDAKCSLNFSTPLE
                     LLVATQLSAQCTDERVNLVTADLFKKYRAVEDYATVGQEEFEKDIKSTGFYRNKARNI
                     RAAAQRIISEYGGEVPREMNAILTLPGVARKTANVVLGNAYGIVEGVVVDTHVGRLTR
                     RFGWTTNEDPVKVEQDLMRILPRKDWLDASHLMIYHGRALCLARKPLCAQCDLARLCP
                     SAFRV"
     gene            2555..2782
                     /locus_tag="VGT44_21925"
     CDS             2555..2782
                     /locus_tag="VGT44_21925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583535.1"
                     /translation="MKQRWFRALGWIYYPVSWQGFLLVLLVLAFCVQVFLAVDSRSHS
                     VSDTLYGIFPYVVPSLLVLLWIASKTSAREG"
     gene            complement(2886..3158)
                     /locus_tag="VGT44_21930"
     CDS             complement(2886..3158)
                     /locus_tag="VGT44_21930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583536.1"
                     /translation="MQQIPATNDADREYQDGYSRIMSFAAYAQSRGWRLSDRQLVHEI
                     MQRERAAMIRDKSSLPIVGRGIHSAAYNRGQADALRSLLREQRQTK"
     gene            3327..3400
                     /locus_tag="VGT44_21935"
     tRNA            3327..3400
                     /locus_tag="VGT44_21935"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3361..3363,aa:Glu,seq:ttc)
BASE COUNT          766 a         1148 c          967 g          774 t
ORIGIN      
        1 tccgggtcac gcgcctccac cagggcgcaa tgaatggcgc ccctacacgc cacatcaacc
       61 tggacaaaac gctacaattg ttgagccatg ccaaacgtgg tacaatcatt gaaaaccgca
      121 tagtccaggg aaggagtcac acgcgcaatg gagccagcac acttctccgg ccaaaccctc
      181 gtcctgctca atcctgccgc aaatcggggc aatatgcaca ggcaccgcgc cgccgtgcgc
      241 cagcgcgcag agcaggcggg agcggagtac atcgaaacca cgctgcaggg cgaggccaaa
      301 gagcgcgcgc agcaggccgc cgccgaagat cgtcccatta tcattgtcgg cggggatggc
      361 tccgtacacg aggccgtcaa tggtatcctg gcgagcggca ggcgcgtccc cctgggcatc
      421 gtcgcggctg gttcaggcaa cgacttcgcc tggaacacgc tcaaactgcc gcacgacccg
      481 caggcagccg tcgaacgcgc cttcaacggg gaaccggtgg aggttgacgc gggtgtggtc
      541 aatgaacgct acttcgccaa ttcgttcagc gtgggcatcg atgccgacat cgcggtcgcg
      601 gcgggcaaga tgaagcgcta ccccttcatg agcggcgcgc ggctctacta caccaccacc
      661 atcaaacagc tactctttgg ctacggtcgc tgtccctggc ttgcattcga gctggatggc
      721 ggcgatgtga tccaggctgg cgagcggcgt tacattttga tggctgtcac caatggtccc
      781 acctacggcg cgggcttccg catcaacccg cgcgccgatc atgccgacgg tctgttcgac
      841 atctgcgcca tcagccacac gccgctcgcg cgcgccctca agctgctgcc ggtcgttcaa
      901 aaaggagaac acgaatcgct gcccgaagtg actttctacc acgccagaac cgtctcaatt
      961 aaaagtcgtc tgcccgtcaa catccagatg gatggagaaa ccacgcgcgc caccagcttt
     1021 catgccgaat gcctgcccgg cgcgctctta gtgcgagtgt aattacaaca tgctctacaa
     1081 tgtcctgcgc gccctcttct ggggctttac ccacctcgtc tgtcgttatc gtgttgtcgg
     1141 cctggagcgc gtgccatcga gcggttcgct gttgatcgtc gccaatcacc tcagcttcta
     1201 tgacccgatg ctgctcggcg tcatcctgcc gcgccgcgcc tggatcttca ccaaagccga
     1261 gatctttcgc tggccgatcg ccggggctgc ctgcaaatat accggccaga taccggtgcg
     1321 gcgcggtgaa agcgaccgca gcgccctcga aaaagccctg gtctacctgc aagaaggcaa
     1381 agccctgatg cttttccccg aaggaacggt cgaacgccag gagcagatga tccccgcgcg
     1441 cgccggggcc gccatgctcg ccgctcgcag tggcgctacc atcctgccca tcgcccatgt
     1501 cggtacgcgc cgcatcctgc gcaccccgcg cagctggttc ccgcgcgtca ccatcaccat
     1561 cggcgaaccc ttcacaccca ccatcccaac cgatgtgccg cgcaaagtgg gcctgcaaca
     1621 cgtcaccgat gagatcatgc agcgcatcgc cactatgctt cccccggaaa atcgcggggt
     1681 gtacgcggaa aacttactct gagtcaacct cctggttctc gttttcggtt gctcggccat
     1741 agagccaggt tacactctaa acgccgacgg acacagtcgc gccaggtcgc actgtgcgca
     1801 caacggcttg cgggcaaggc atagggcgcg tccatgataa atcatcaaat gcgaggcgtc
     1861 cagccaatcc ttacgcggca gaatacgcat caaatcctgc tccaccttga ccgggtcctc
     1921 gttcgttgtc cagccgaaac gccgcgtcag gcgtcccacg tgcgtatcca ccacgacacc
     1981 ctcgacaatg ccataggcat tccccagcac cacattggcc gttttgcgcg ccacgcccgg
     2041 cagtgtcaat atggcattca tctcgcgtgg cacctcgcct ccatactcgc taataatgcg
     2101 ttgagccgcc gcgcggatat tcctggcctt gttgcgataa aaccccgttg atttgatatc
     2161 cttttcaaac tcctcctgcc ccaccgtcgc atagtcctct accgcccgat atttcttgaa
     2221 caggtcagcc gtcactagat tgacgcgctc atccgtacac tgcgccgaca actgcgtcgc
     2281 caccagcaat tccagcggcg tcgaaaaatt gagcgaacac ttcgcatcgg ggtataaccg
     2341 ccgcagttcg gccatgattg ccgtgacctg ttccggcgag cctggggtcg gcccggcgta
     2401 tgtgggagta tctaattcca tagaacatcc ttcctgttct tatttttgcc tcattataac
     2461 atagacccat ctgcactcaa gtgcttgccc ttcgaccctc ttttcattta ctatgtagca
     2521 tagaagttgt tgctcaattc aagcaggagg acttatgaaa caacgctggt ttcgcgcgtt
     2581 gggctggatc tattaccccg tttcgtggca ggggtttcta ctcgttttgc tcgtcctggc
     2641 cttttgcgtg caggtattcc tggccgtaga cagccgctcg cattcggtca gcgatacgct
     2701 ctacggtatt ttcccctacg tggtaccgag cttgctggtg ctattgtgga ttgcatcaaa
     2761 aaccagcgcg agggaagggt aaactgctgc tcgtattggc tgatgaggcg cgggattcag
     2821 caaatataaa taaattgaag ccattacctt gtgagaggag gatgcccgcc acacacgctt
     2881 agtcgctact tcgtctggcg ctgctctcgc aggagcgacc gcagggcgtc ggcctggccg
     2941 cgattgtagg cggcggaatg gatgcctcgg ccaacgatgg gcagggaact cttatcgcgg
     3001 atcatggcgg ctcgctcgcg ctgcataatc tcatgtacca gctgccggtc actcaagcgc
     3061 cagccgcgcg attgcgcata cgccgcaaac gacataatgc ggctgtaccc atcctggtac
     3121 tctcgatcag catcgttggt tgccggtatc tgttgcatgg ctcaatctta ctccgaatgc
     3181 gccgccgcag cgcttgatat gctctacgtt gtgctatcac cattatgaca aaaaagtggg
     3241 gagaatgtca atacacaatt tttcttgaca aagcgcattg ccatagagta caatcatgtc
     3301 gccgctacag aagtagcttc agacatgcct cgttcgtcta gtggtccagg acgccgccct
     3361 ttcaaggcgg agatcacggg ttcgactccc gtacgaggta ctattttcag aaaagcctgg
     3421 tacgcccgtg atatttggtg tgccaggctt ttctgcttgc catgtttgca acccatcgta
     3481 gggacgcaat gatacaactg taggtatcga tttatcgcgc acatcgccga tttatcggcc
     3541 aaatgtacgt tgtacagcag gcatccaggc cgataatgaa ctttaacaaa ttaatccggc
     3601 aataagccaa cgtagtgcag caagtcggga gagggagact catactgagc caacc
//
LOCUS       DASZAK010001247         3419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_388025, whole genome shotgun sequence.
ACCESSION   DASZAK010001247 DASZAK010000000
VERSION     DASZAK010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388025"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..614
                     /locus_tag="VGT44_21940"
     CDS             240..614
                     /locus_tag="VGT44_21940"
                     /inference="COORDINATES: protein motif:HMM:NF024757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HEV2583537.1"
                     /translation="MCNQNDTREVARQAERRFFSHSLSAPGIGWYRASFTLSLPSNMA
                     VPIGLHIADATYHYRALIFVNGWLIGRYWNTVGPQNTFYLPAGILNTRGTNDVAIAVW
                     GLDAPGGGLGQVSLTSYGQYTQ"
     gene            complement(846..1721)
                     /locus_tag="VGT44_21945"
     CDS             complement(846..1721)
                     /locus_tag="VGT44_21945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEV2583538.1"
                     /translation="MSVPAAFDYHVAHSVDEAIALMSQYGDDAKFLAGGHSLLPTMKL
                     RLAQPGHLIDLGRIHGLSYVHDDANHIAIGAMTTYATLEKSSVVHQNFPLFTDGIPLI
                     GDQQVRNRGTIGGSVAHSDPAADMPGMVLALKANIHVKGPNGGRTIHADNFFQDLFQT
                     SLEPGEVVTELTFAKRAAHSGSAYEKLANKASHYAVVGCAAVITLDAGGLCTAASVVI
                     TGASVKPSRAGAVEVALVGNHLGEVHIEAAASHAADGLEIVSDIHGSQAYRAQMAAVM
                     AKRAITRAVHRAHGG"
     gene            complement(1735..>3419)
                     /locus_tag="VGT44_21950"
     CDS             complement(1735..>3419)
                     /locus_tag="VGT44_21950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEV2583539.1"
                     /translation="TVWCSSQNPHILRNILSALIGHPLHQVRVIVPEVGGGFGSKIDI
                     YPEEMMAAVISKKLGRPVKWVEDRSENIAVTTHGRDQVDYIEVAATKEGKVTGLKLHG
                     ISDMGAYCQLFTDVIMIAFGLPVACGAYDIKNVHIDADIVFTNKAPTDAYRGAGRPEA
                     TYITERAMDLVARELGKDPAEVRRVNFIQPDQFPYKSAAGAVYDTGDYEKALDKALAL
                     AGYQEMRAEQARLRAQGKLVGIGVSSYIEVCGIGPKGFAPIGLYESARMRVENNGMVM
                     LYTGSSPHGQGEETTFAQIVADEFTISPDQVMVIHGDTDSSPEGRGTYGSRTTAVGGS
                     AAFTAAQKLKEKMKHIASHMLEASPSDVTLEDGKFSVAGSPQKSVSFAEVATTAHFSN
                     TLHPSIEPGLETTVFWEPEANTFPFGTHICMVEIDKETGHVELKRYVAVDDCGRQINP
                     MLVQGQVHGGIAQGVGQAMWEEVIYGDDGQLLTATLMDYAMPLAYGLPHFELASTVTP
                     TTINPLGVKGVGEAGTIGSTPAVSNAVIDALGVAHLDMPYTSEKIWNVIHSK"
BASE COUNT          683 a          968 c         1037 g          731 t
ORIGIN      
        1 gattcaggcg gcggagccga tccacaatcg ccctcgtttc ttctgtcacg accccttcgg
       61 caacctgttg gagttcacga cgattctggg cgattacctg gatgaacaga cgggcgagtg
      121 aaagaccctt tgcatcgccc cactcgccca ctgttattgc cccatccgct gtcatcgccc
      181 cacccgctcg ctgtcatcct gagcgcagcg aaggatctga gactgtgtga aaagcgctca
      241 tgtgcaatca aaatgacact cgagaagtgg ctcgacaggc cgaacgacga ttcttttcac
      301 acagtctctc tgcgcccggc atcggctggt atcgcgccag ctttacgtta tctctcccct
      361 caaacatggc tgttccgatt gggctgcaca ttgccgacgc cacctatcat taccgggcgc
      421 tgattttcgt taacggctgg ctgattgggc gatactggaa tacagtcggc ccgcaaaaca
      481 cgttttacct gccagcaggc atcctgaata cacggggaac aaatgacgtg gcaatagcgg
      541 tatggggtct cgacgcgcca ggagggggat tgggtcaggt gagcctgacc agctacgggc
      601 aatatacaca atgaagagaa gttgctaata ctgaagtatc acgaaggata atgaagtttt
      661 cacgaacaac atcggtaata gcttgtagga gccgattgat cggctaatac atgcaaaatg
      721 aaatgatatg cataggagcc gataatggag attaacaaaa tcctgcggct atccgagacg
      781 ggaggacgcg agggcgaccg caagggtgga gacccctgcg gtcgccctcg tcttcccagt
      841 tcaggctaac cgccgtgtgc gcggtggacg gctcgcgtga tggcacgttt cgccatgact
      901 gccgccatct gagcgcgata cgcctgcgag ccgtggatat cggaaacgat ctccaggccg
      961 tctgctgcgt ggctggcggc ggcctcaata tgcacctcgc ccaggtggtt gccgacgagc
     1021 gctacctcga ctgcgccagc tcgtgagggt ttgaccgatg cgccggtgat gaccacgctg
     1081 gcggcggtgc agaggccacc ggcatccagg gtgatgacag cggcgcaacc cacgacggcg
     1141 tagtgtgagg ctttgtttgc cagcttttca tacgcgctgc ccgaatgagc ggcgcgtttg
     1201 gcgaaagtta gctcggtcac gacttcccca ggctcaaggc tggtctggaa gaggtcctga
     1261 aagaaattat cagcgtggat ggtgcgtcca ccattgggac ccttgacgtg gatattggcc
     1321 ttgagcgcca gcaccatacc gggcatatcg gcagccgggt cggagtgcgc gacgctgccg
     1381 ccgatggtgc cgcgattgcg cacctgctga tcgccgatta gcgggatgcc gtccgtgaac
     1441 agcgggaaat tctggtgaac gacgctcgat ttctccagcg tagcgtaggt cgtcatcgcg
     1501 ccgatagcga tgtggttcgc gtcatcgtgg acataggaaa gcccgtggat gcggccaaga
     1561 tcgatcaggt ggccaggctg ggccaggcgc aacttcatgg tcggaagcag gctatgccca
     1621 ccggccagga acttggcgtc gtcgccgtat tgcgacatga gcgcaatggc ttcatccacc
     1681 gaatgggcga cgtgataatc aaatgcagcg ggaactgaca tgctactttc tcctttattt
     1741 cgagtggatg acgttccaga tcttctccga cgtatacggc atatcgaggt gcgccacgcc
     1801 gagcgcatcg atcacagcat tggaaaccgc cggtgttgaa ccgatcgtgc cggcctcgcc
     1861 cacgcccttc acacccagcg ggttgattgt cgtcggcgta acggtcgagg caagttcaaa
     1921 gtgcgggagt ccatacgcca gtggcatggc atagtccatc agcgttgcgg tcaaaagctg
     1981 gccgtcatcg ccgtagatga cttcctccca catagcctgc ccgacgccct gcgcgatgcc
     2041 gccgtggacc tggccttgca caagcatagg gttgatctgg cgtccgcaat cgtcaactgc
     2101 aacatagcgc ttgagttcga cgtggccggt ctctttgtcg atctcgacca tgcagatgtg
     2161 cgtaccgaat gggaaggtat tcgcctctgg ctcccagaag acggtcgtct ccaggccggg
     2221 ttcgatgctg ggatgcaggg tattggagaa atgggcagtc gtggccactt cagcgaacga
     2281 gaccgatttc tgtggagaac cggcaacgga gaatttccca tcctcgagcg tgacatcaga
     2341 agggctggct tcgagcatat gggaggcgat gtgtttcatc ttttccttca gcttctgggc
     2401 agcggtgaag gccgctgatc cgccgactgc tgtggtgcgg ctgccatagg tgccgcgtcc
     2461 ttcgggtgaa gagtcggtat cgccgtgaat aaccatgacc tggtcggggg agatggtgaa
     2521 ctcgtcggcg acgatctgcg cgaaggttgt ttcttcgccc tggccgtgcg gcgaggagcc
     2581 ggtgtagagc atcaccatcc cgttgttctc cacgcgcatt cgggcgcttt catacagccc
     2641 gataggagca aatccctttg gtccaatgcc gcaaacctcg atataggagg agacgccgat
     2701 gccgaccagc ttgccctggg cgcgcagccg agcctgttcc gcgcgcattt cttgatagcc
     2761 agcgagcgcc agcgctttgt ccagggcttt ctcataatcg ccggtatcgt agactgcgcc
     2821 tgccgcagat ttgtagggga actggtcggg ctggatgaag tttacgcggc gtacttctgc
     2881 cgggtctttg cccagttcgc gcgcaacgag gtccatagcg cgctcggtga tgtaggtcgc
     2941 ctccgggcgg cccgcgcctc gataggcatc ggtaggcgcc ttgttggtga agacgatgtc
     3001 agcatcgatg tggacgtttt tgatatcata cgcgccgcag gcgaccggta agccgaaggc
     3061 gatcatgatg acatcggtga agagctggca ataagcgccc atatcggaga tgccatgcag
     3121 cttgaggccc gtcactttgc cctcttttgt cgcggccact tcaatgtagt ctacctggtc
     3181 gcgtccatgt gtcgtgacgg caatgttttc gctgcggtct tcaacccatt tcaccgggcg
     3241 gcccagcttc ttcgagataa cggcggccat catctcttct ggatagatgt cgatcttgct
     3301 gccgaagccg ccgcccactt caggcacgat cacgcgcacc tggtgcagcg ggtgtccgat
     3361 caacgcggac aggatatttc gcaggatgtg cgggttctgc gaagaacacc agacggtca
//
LOCUS       DASZAK010001248         5149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_493139, whole genome shotgun sequence.
ACCESSION   DASZAK010001248 DASZAK010000000
VERSION     DASZAK010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493139"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1818)
                     /locus_tag="VGT44_21955"
     CDS             complement(7..1818)
                     /locus_tag="VGT44_21955"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2583540.1"
                     /translation="MGRRLGLLIGINHYQDSTFQPLRYAEPDASMFARWLGNERGGDW
                     PSSEIYTLTGAEATKEQVESLVAQLCLSAAQPDDLVLIYFAGHAFIDEASGDGLLASV
                     HTSYRRPASAIHLPSLVRQVMLPSRAGQVIVLLDCFQTGGVWNAQRATPYDFRPFIGQ
                     PSTIALQQAQERILYCSCRGNDLAPESGEQGCGSFIYRTIIGLSGPARNAATGQATLQ
                     QLHTYLSGKLSPQHQPIGFGQSATPIVLIGEAPVYAGVASPAYAASSSVSQSAYSLNG
                     QAGQVTFQGVGESAPLIAPDTPRASHATGQLASSTSGPISISQVEQNYQQQCLKLMQQ
                     ARQQLGAQRLPEALQLVEQVLRMAPNYSDALTLKTQILGTTARYQEALATNELLLQIE
                     PGNALAWSMYAALLTNTGRLQEALTAINRALTLQPGNQEALAMRQSIQSSLPAQLPGT
                     SASARSPYATRQPARESAASFLVGAGIQLLAFILGLLGAAIQVYLPSLPNALGFLLES
                     FGLAGLCVLAARGSFRYGASRLLSTSLLCIAAAAILGGLYKFEYVALINKIVATPSLL
                     VPIVFFAIWLIAAASLPLLAGLGGLILGLVMGVRRKR"
     gene            2019..2624
                     /gene="coaE"
                     /locus_tag="VGT44_21960"
     CDS             2019..2624
                     /gene="coaE"
                     /locus_tag="VGT44_21960"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV2583541.1"
                     /translation="MPRIIGVTGNIACGKTSVGQMLLEMGAERYIDADAVVHRLYQAG
                     QAIAVQVAVTFGPAVMYADGSVNRAALGEVVFHDAALMQRLESIVHPAVGVAIAQELA
                     EVSPTGIAIIDAVKLLEGGSAMFCQSKWLVICREEQELARLMARNQLSREEAEARLRA
                     QPSVEARIRLVDEVINNGGSLDETRTQVAAAFARFRTRFPA"
     gene            2910..4256
                     /locus_tag="VGT44_21965"
     CDS             2910..4256
                     /locus_tag="VGT44_21965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2583542.1"
                     /translation="MKRRWSRIFSLALGIPLMLAILAACGSGTTGTGGNTPTAGSTTI
                     KIATELPVSGKDESSGKPTENGAHLAVDQANANHTIPGYTLVFDPKDDVGPSGTNDPV
                     VGAANIRALIGDALVSAVVGPFNSAVAKSEMPISNLAPLAQISPANTNTCLTQEGAAV
                     GCSGATDLVPTLRPSGKVTYFRIATTDSHQGPAGADYLYKTKGYHSVYIIDDTTTYGV
                     GIADAFSKEWTTDGGTVLGRSSEPPTTTSYVSLLTQIASKHPSVIYFGGLDSTGGILI
                     RQQMEQVPALANLPFGGGDGLVTSTFSKTIGTSGGGPIFGTVATIDVASAPGSQAFLS
                     AYDSTYGVSNLGAYSAAGYDCANIIIQAIKAALAGGAKTPTSSSDAAGAKLFRQAVIN
                     AIQNIQFTGVLGPQAFDANGDTTNRVITVYQVGPNPSGIPFSTPGWNPVAAVKIGS"
     gene            4419..>5149
                     /locus_tag="VGT44_21970"
     CDS             4419..>5149
                     /locus_tag="VGT44_21970"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV2583543.1"
                     /translation="MTLFLQELIVGLTTGAIYALIALGYTMVYGIIELINFAHGDIFM
                     IGTFVSITIFGLLGITSTSHPAGLALVTILVVTCLGSMLICAVLGVVIERVAYRPLRN
                     APRLAPLISAIGVSLILENFGSLWKGVSYISFPQIFPQITYTLVNTANGPVTISSVNL
                     LVLVVALVLMVGLQWMVTSTRIGRAMRAVAQDREAAALMGVNVDRIIAITFFIGAGLA
                     GAAGFIYGLQYGSSIFYIGFQLGLIA"
BASE COUNT         1063 a         1360 c         1553 g         1173 t
ORIGIN      
        1 tgtcggctac ctcttcctgc gcacacccat caccaggccg aggatcagcc cgcccagtcc
       61 cgccagcagg ggcaggctgg ctgccgcgat cagccagatg gcgaaaaaga cgatgggtac
      121 gaggagcgac ggggtagcta cgattttatt gatgagcgcg acgtactcga acttgtacag
      181 gccgcccagg atggcagccg cagcgatgca gagcagacta gttgaaagca ggcgactggc
      241 gccgtaccgg aagctgcccc tggcggcgag aacgcacagt ccggccagtc cgaagctttc
      301 cagcagaaag ccgagagcgt tgggaagcga ggggaggtag acctgaattg ccgcaccaag
      361 cagccccagg atgaaagcca ggagttgtat tcccgcgccg actagaaagg aagcggcact
      421 ctcacgcgct ggctggcgag tcgcataggg agatcgcgca gaggcggagg tgcctggcag
      481 ttgagcgggc aggctggatt gaatagactg gcgcatggcg agagcttctt gattgccagg
      541 ctgaagggta agcgcgcgat taatggctgt cagcgcctcc tggagcctgc ccgtgttggt
      601 cagtagtgcc gcgtacatgc tccaggcaag cgcgtttcca ggttcaattt gaagcagtag
      661 ctcgttcgta gcaagcgcct cctgatagcg agcggtggtg ccaagtatct gtgtctttaa
      721 ggtcagcgca tcagagtagt tgggggccat acgcagcacc tgctcgacca gttgaagagc
      781 ctcgggcagg cgctgagcgc cgagctgctg cctggcctgc tgcatcagct tgaggcattg
      841 ctgctggtaa ttctgttcca cctgggagat ggaaattggg ccagacgtcg atgaagcgag
      901 ctggcctgtc gcgtgacttg cgcgaggcgt atcgggggcg atcagcggcg cgctttcgcc
      961 gacgccttga aatgtgacct ggccggcctg accgttaagt gagtatgccg attgtgatac
     1021 cgacgaggag gccgcatatg ctggtgacgc gacccctgca tagaccggag cttctccgat
     1081 cagcacgatg ggcgtggcac tctgcccgaa gcctatcggt tgatgttgtg gcgacaattt
     1141 accggacaga taggtatgaa gttgttgcag cgtggcctgc ccggttgcgg catttcgagc
     1201 gggaccgctc agcccgataa tcgtgcggta gatgaagctg ccgcatccct gttcgcccga
     1261 ctctggagcc aggtcgttgc cccggcatga gcaatagaga atgcgttctt gagcctgctg
     1321 cagggctatg gtggaaggtt gcccaatgaa cgggcgaaaa tcgtaggggg tggcgcgctg
     1381 tgcgttccag accccgccgg tctggaaaca gtcgagtaga acgatcacct gtccggcgcg
     1441 gctgggaagc atgacctgcc tgacaaggct gggaagatga atggcggaag cggggcggcg
     1501 ataggacgta tgaacgctgg cgagcagtcc gtcaccgctg gcttcatcga tgaaggcgtg
     1561 tcccgcgaag tagatcaaga caagatcgtc aggttgagca gcgctgaggc agagttgtgc
     1621 gaccaggctt tctacttgct cctttgtcgc ctccgcgccg gtcagtgtgt atatttcaga
     1681 ggaaggccag tcacctccgc gttcgttccc aagccaacgc gcgaacatgg acgcatccgg
     1741 ttcagcgtag cgcaggggct ggaacgtgct gtcctggtag tgattgatgc cgatgagtaa
     1801 tcccaggcgt ctgcccacgc tgtaactcct ttcttctgaa tactcgcttg actacaggct
     1861 gattgctgac gaacacactt ttgtaattgt accgtaatgt gcgtaaagtt tgggcatttc
     1921 tgggaaaaat caggtacttt tggcgattcc gataaggtat gtacgggtgt gatatactct
     1981 gtcagcgcgc attatatcca gggaaggatg catcgatcat gccacgtatc attggcgtca
     2041 ccggcaatat cgcctgcggg aaaacatctg tagggcagat gctgctcgaa atgggcgcgg
     2101 aacgctatat tgatgctgac gcggttgtgc atcggctcta ccaggcggga caggcgatcg
     2161 cggtacaggt agcggtgacc ttcggaccgg cggttatgta tgctgacggg agcgtgaatc
     2221 gcgcagcgct gggcgaggtg gtttttcacg atgcagcgct gatgcagcgc ctggagtcga
     2281 tcgtacaccc ggcggtggga gtcgccattg cccaggagct tgctgaagtc agtccgaccg
     2341 gcatcgccat catcgacgcg gtcaagctgc ttgagggcgg ttccgccatg ttttgccaga
     2401 gcaagtggct ggtgatctgc cgcgaggagc aggaactggc gcggttgatg gcgcgcaatc
     2461 agttgagccg ggaagaggcc gaggctcgct tgcgcgcgca gccctctgtg gaggcgcgca
     2521 taaggctggt cgatgaagtc atcaataatg gcggctcgct cgacgagaca cgcacgcagg
     2581 tggctgccgc attcgcgcgt tttcgtaccc gttttccagc atagaaactg cggaaaattt
     2641 ctattgacag cagcgaatgg gccacgtata atttgctcat ccataacact ctttgtttcc
     2701 tggttcacgg gtggtattcc tttaagcaca tcgaactcta tcaaagcaga acaatatagc
     2761 tcttaagaac agtgagattg agatgcttta cgacgtagtt atcacatgag ggggtgttgc
     2821 ttctcttaat tgggtgcagc gcttgcgccc ctcgcgaacc cattgagcat gtggtttatt
     2881 tggtaaattt taaggaggct gaccaggtca tgaagagaag atggtcacgt attttttcgc
     2941 tggcactggg tattcccctg atgctggcga tactggctgc ctgtggttca ggcacgactg
     3001 gcactggtgg caatacgccc accgcaggtt ctaccactat caagattgct accgaactgc
     3061 cggtatccgg caaggacgag agcagtggca agcccaccga gaatggcgcg cacctggcag
     3121 tagaccaggc taacgccaat catacgatcc ccggctatac gctggtcttt gatcccaaag
     3181 acgatgttgg cccgagcggc acgaacgatc ccgttgtcgg tgcagcgaac atcagagcat
     3241 tgattggcga tgcgctggtg tccgccgtcg ttggcccctt taacagcgcc gtagcgaagt
     3301 cggagatgcc gatcagcaac ctggctccgc ttgcacagat tagcccggcc aacacgaata
     3361 cctgcctcac gcaggaaggg gcggctgtcg gctgcagcgg cgccaccgat ctggtaccga
     3421 ctctgcgacc ttcaggcaag gtcacttact tccgtattgc caccacggac agtcaccagg
     3481 gacccgcagg ggctgattac ctctacaaga caaaagggta ccacagtgtg tatatcatcg
     3541 acgataccac gacttacggc gtgggtatag ccgatgcctt cagcaaggag tggacaacag
     3601 acggtggaac agtgctggga cgcagtagtg aacctcccac gaccacttcc tacgtctccc
     3661 tgctcacgca gattgcttcg aagcaccctt ctgtgatcta ctttggcggc cttgactcta
     3721 ccggcggtat cttgatccgc cagcagatgg agcaggttcc ggcattggcg aacctcccct
     3781 ttggcggtgg tgatggcctg gtgacgagta ccttctccaa gacgattgga accagcggcg
     3841 gcggcccaat ctttggtacc gttgcaacca ttgatgtggc ctcggcgcct ggttcgcaag
     3901 ccttcctcag cgcttacgac tcaacctatg gtgtatccaa ccttggggca tacagcgctg
     3961 cgggctatga ttgtgccaat atcatcatcc aggcgatcaa ggctgcgctg gcgggtggcg
     4021 cgaaaactcc cacgagttcg agcgatgctg ctggagccaa gctcttccgc caggcggtga
     4081 ttaacgcgat ccagaacatc cagtttacgg gtgtcctggg accgcaggct ttcgatgcga
     4141 atggggatac caccaatcgg gtcatcacgg tctaccaggt cggtcctaat ccgtcaggta
     4201 tacctttcag cacccctggt tggaatccag ttgcggcagt gaagatcggg agctaatttt
     4261 cgatgacgag ggccaggtgg atgtgaactg aataggcgcg acatctggct cacagatcag
     4321 gcgggggata gtcggcgtat ctgcgctgta agactacccc cctgcttgtt attttttaac
     4381 cttaggagtg tcgcctgtat attttaggaa ggtaagctat gaccctgttt ttgcaggaac
     4441 tcatcgtagg gctcaccacc ggagctattt acgctctgat tgctctgggg tatacaatgg
     4501 tctacggtat catcgagctg attaactttg cgcacggtga tatcttcatg atcggcacgt
     4561 ttgtttcgat aactattttt ggactcctgg ggataacctc tacatcgcac cctgcaggat
     4621 tggcgcttgt aacgatactg gttgttacct gtctgggttc aatgctcatc tgtgccgtac
     4681 tcggtgtggt aattgaacgt gtggcgtatc gtcccctgcg caatgctccc cgtctcgccc
     4741 cgctgatctc tgccattggt gtctcattga ttttagaaaa ttttggatcg ctctggaaag
     4801 gtgtctccta catctccttt ccacagattt tcccccagat tacctatact ctggttaata
     4861 ctgctaatgg tccggtaact attagcagtg tcaatttact ggtactcgtt gttgcactcg
     4921 tgctgatggt tggcctgcag tggatggtta ccagcacacg catagggcgt gcgatgcgtg
     4981 cggtggccca ggatcgtgaa gcggctgcgc tcatgggagt aaacgtcgac cgcatcattg
     5041 cgatcacctt ctttatcggt gcggggcttg ccggagcagc cggtttcatt tacggcttgc
     5101 aatatggcag ttccattttc tatattggtt tccaacttgg tctaattgc
//
LOCUS       DASZAK010001249         3059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_459628, whole genome shotgun sequence.
ACCESSION   DASZAK010001249 DASZAK010000000
VERSION     DASZAK010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459628"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VGT44_21975"
     CDS             complement(<1..753)
                     /locus_tag="VGT44_21975"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HEV2583544.1"
                     /translation="MNLFEKQSRLNQLFTQSPVNAAYLAGSLSTRTSFGHLTDVDIAI
                     LLMEQIKSDQFLDYQLYFFSELAKRLESDNIDVVILNQASMLLKLQVIKYGQILFSRD
                     EKMRISFETKAVMDYLDFKRFDEIQNQALSRRLYGQILPVDKELAQRYLKQAREAVTI
                     LRDLGKTDREEFTSDYRLFGLAERYLQVAIDSCLRLCNVLVSSLGLRRPDGFHELLSI
                     IAGQQIIPQTLAYRLEVLTDFRDALVHDPSALN"
     gene            complement(909..1322)
                     /locus_tag="VGT44_21980"
     CDS             complement(909..1322)
                     /locus_tag="VGT44_21980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEV2583545.1"
                     /translation="MEPVILITREPLDRDVLIASVGDPGVGGIVIFEGVVRDNARGKQ
                     VRYLEYDVYPEMAIAQIRAIVTEAEQRWGIERVAVAHRVGRLEIGEASVIIVVASPHR
                     SEAFDACRYIIDTLKTSVPIWKKEVATNGEEWVEG"
     gene            1684..2544
                     /locus_tag="VGT44_21985"
     CDS             1684..2544
                     /locus_tag="VGT44_21985"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV2583546.1"
                     /translation="MSHEDQPVERSVGSFQDQEGLHPSPLKDYLDEAGVADPFALWAA
                     PSDQPLAPLPTSETSTRRPGGRRTKSDPRSASRPVQQNRRKLVKLLAVGTVGIVTVVT
                     VGGVSFARSPLSPTRSPSRLGDDQDEGSSSIAAHRERDDWEEEGRGKPPHKTPTPTKS
                     PTPKPTPSPTKTPSPTATPPTATPTPSGTVIGSTTQATNTAVAFTNPADGQASWLVHM
                     SNGNFVAVEQACTHAGVPVKYNASSGQFNCPAHGAIFNADGTNPQVPATRPLPPVHIT
                     VNANGTITVP"
     gene            2694..>3059
                     /locus_tag="VGT44_21990"
     CDS             2694..>3059
                     /locus_tag="VGT44_21990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583547.1"
                     /translation="MMIQQVGSETREEEETAKGEGNVQQQETGTCPVETAFRPFTRVE
                     RRAVWFKEYGEQDVALHMWTRLVEQQDLEIEMLLQMHGLLVFGVLVSTLRYVQFYIDL
                     NEELHREREPETADALRDYY"
BASE COUNT          667 a          825 c          902 g          665 t
ORIGIN      
        1 gttgagggca ctggggtcat gcaccagggc gtcgcggaag tcggtcagga cttccaggcg
       61 ataggccagc gtttgcggaa tgatctgctg tccggcgatg atgctcaata gttcgtggaa
      121 tccatccggg cggcgtaagc ccagcgagga taccagcacg ttgcaaaggc gcaggcacga
      181 gtcgatggca acttgcaggt agcgttcggc cagcccgaag aggcgataat cgctcgtgaa
      241 ctcttcgcga tccgttttgc cgaggtcgcg caggatggtg acggcctcgc gcgcctgctt
      301 caggtagcgc tgcgccagtt ctttatcaac cggcaggatt tgcccgtaga ggcggcgact
      361 gagcgcctgg ttctggatct catcgaagcg cttgaagtcc aggtaatcca tgaccgcttt
      421 ggtctcgaac gagatgcgca ttttctcatc gcgactgaac agtatctgcc catatttgat
      481 gacctgcaac ttgagcagca tggatgcctg gttgaggatc accacgtcga tattgtccga
      541 ttccagccgt ttcgccagct cgctaaagaa gtagagctgg taatcaagaa actggtcgga
      601 tttgatctgt tccatcagga gaatggcgat atcaacgtcg gtgaggtgtc caaaagaagt
      661 gcgtgtcgaa agagagccgg ccaggtacgc ggcattgacg ggactctgcg tgaagagctg
      721 gttgagccgg gattgttttt cgaataagtt catccatacc tccgggagaa tgcccaggct
      781 agcgcaagct gcttcaggat gccagggcga ccgcgagggt cgccccctac ccttcagtgt
      841 gccagggcga ccgcgagggt cgcccctacc cttatggata taactatacc atatggaaaa
      901 gcccggtgct atccttcgac ccattcttcg ccgttggtag cgacttcctt tttccagatg
      961 gggacgctgg ttttgagggt atcgatgatg tagcggcagg cgtcgaaggc ttcagagcgg
     1021 tgtggcgagg cgacgacgat gatgacgctg gcctcgccga tttccaggcg gcctacgcgg
     1081 tgcgcgacgg ccacgcgctc aatgccccag cgctgctcgg cctcggtcac gatggcgcgt
     1141 atttgcgcga tggccatttc ggggtagaca tcgtattcga ggtagcgcac ctgtttgccg
     1201 cgcgcgttat cgcgcacaac tccttcgaag attacaatac ctccgacgcc tggatcgccg
     1261 actgaggcaa tcagcacatc gcggtcgagt ggttcgcggg tgatgagaat gacgggttcc
     1321 atatctcccc tttctgacgg gttttgggcg gctttctcat gtattcagcc agtctgatcg
     1381 ctctttcatg aacaagcata tcatacagcg gcttcgtttg ctgcctctgt tattgtacag
     1441 ggttgtgttg tactcggaaa agaacagttt catgcgcgag aggacagagg ttagctaatt
     1501 tttgtaaaga gtccagatct ctctcctcct tttcagtcct gtgttatgca cggcgtcttg
     1561 acgctctacc aaccttccag gtaccttgac gtttgttagg tagtttcgaa ctgctcgtcc
     1621 ggtcaaccct gaagagttat gagcagtaaa ggatgtattt tgaaaggagt tgttgtattg
     1681 aacatgtcac atgaagatca acccgtagag cgatcagtcg ggagctttca agatcaggaa
     1741 ggtctgcacc cttcaccatt gaaggattac ctggatgagg ctggagtcgc tgacccgttt
     1801 gcgctgtggg ctgctccatc ggaccagccc ctggccccgc taccgacgtc tgaaacatcc
     1861 acccggcgtc ctggagggcg taggacgaag tctgatccac gctccgcttc tcggcctgtg
     1921 cagcagaatc ggagaaaact cgtcaagctc cttgcagtgg gtaccgttgg aattgtgacg
     1981 gtggtcacgg tcggtggagt tagctttgcc cggtctcctc tcagcccgac gcgttcacca
     2041 tcgcggctcg gcgatgatca ggatgagggg tcaagctcaa tcgctgcaca tcgagagagg
     2101 gacgattggg aagaggaagg gagagggaaa ccgccgcata agaccccgac tcctaccaag
     2161 tcgcctaccc caaagcctac accttcgcca acgaaaacgc cctcgccaac agcgacacct
     2221 ccaaccgcga cgccaacacc gtcaggaacg gtgattggtt cgactaccca ggccacgaac
     2281 accgccgtcg ctttcaccaa tcccgccgat ggccaggcga gctggctggt ccatatgagc
     2341 aatggcaact tcgttgccgt tgaacaggcc tgcacacacg cgggtgtccc ggtcaagtac
     2401 aatgcctcct cagggcagtt caactgtccg gctcatggcg cgatttttaa tgcggacggc
     2461 accaaccccc aggtaccggc gaccaggcct ctgccgcctg tccatattac tgtcaatgca
     2521 aacgggacga ttacggtgcc gtaactggca acgtaaccgt ttctccgtgt tgagcgcgct
     2581 ctcgaactgc cggaatggac tgggagattt ggagattgtt cgggagcgcg tctatgcagg
     2641 tgcagcgaaa actgagttca gaggcgctta acagaaagta agacacacac gctatgatga
     2701 tacaacaggt cggttctgaa acgagagagg aagaagagac ggcaaaggga gagggaaacg
     2761 tacaacagca ggagacaggc acgtgtccgg ttgaaacggc ttttcgtcca ttcacgcgcg
     2821 tcgagcggcg agccgtgtgg ttcaaggaat atggtgagca ggacgtcgcg ttgcacatgt
     2881 ggacgcgact ggtagaacaa caggacctgg aaatcgagat gctgctccag atgcacggcc
     2941 tgctggtctt tggcgtgctg gtcagcacac tgcgctacgt ccagttttac atcgatctaa
     3001 atgaagaact acatcgcgag cgcgagccgg aaacggcgga tgcgctgcgc gactactac
//
LOCUS       DASZAK010001250         2257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_350746, whole genome shotgun sequence.
ACCESSION   DASZAK010001250 DASZAK010000000
VERSION     DASZAK010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350746"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..291
                     /locus_tag="VGT44_21995"
     CDS             148..291
                     /locus_tag="VGT44_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583548.1"
                     /translation="MSTSVLVAKERQPGSPFGFGQKQAKKQKNTAQMRHSCALFDVLS
                     IFM"
     gene            468..1910
                     /locus_tag="VGT44_22000"
     CDS             468..1910
                     /locus_tag="VGT44_22000"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurT ligase domain-containing protein"
                     /protein_id="HEV2583549.1"
                     /translation="MIKEDTHSRPVTPSGLERARLALAISAGKLAGASGRLLGVGGGT
                     SLPGLIARRIDPNVLKSVVGASQARKIVITGSNGKTTTARMTAAMAVSNGKRVSHNRA
                     GSNMLQGVTAVAVNFADVFGRLNSDVLLFEIDEGTMPLVMPEIQPDVVVITNIFRDQL
                     DRYGELYSVARALDKTLEDLPETSTILLNGNDPQVANFGQKAKAKRLFFGLESTEVGT
                     PVPEQSADIIRCIHCQEDLVYEVAYMSHLGIYRCPNCGYTLPPLDIAATSIKLAPDGE
                     GPTHVEMRTPHGNMTLDISLPGIHNVYNAAAAIGASLAAGFEKEQLPAALVDIKPAFG
                     RLEKIQAGDQTIYLSFVKNPTSFNLILRLIAQHPGKKHVLLAASHTIVDGEDFSWLWD
                     IDVEAVAQGIADVVCSGNKPEELAMRLKYAEIPVDKISLVEDREAALDAALKNAGPGG
                     ALYILSSYTPTNELRRIMQKRGWVKHTWQE"
     gene            1917..>2257
                     /locus_tag="VGT44_22005"
     CDS             1917..>2257
                     /locus_tag="VGT44_22005"
                     /inference="COORDINATES: protein motif:HMM:NF019305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583550.1"
                     /translation="MSEQVQDTGMVLKIGHLYPTLMSVAADRGNLFSIQKRCQWRGIA
                     TEVEQIYVRQTPDFTKYDLILFHGGADREMELASRDIQAKVPSLIEAAESNTVFLSVC
                     AGFQLLGHYYKP"
BASE COUNT          545 a          611 c          621 g          480 t
ORIGIN      
        1 acctttccga ccggctgagc gaagctagga cgcagtccgt tgccggtcgg aataagattg
       61 agtactacct ttccgaccgg ctgagcgaag ctaggacgca gtccgttgcc ggtcggaata
      121 agatcgagtc attcaggagt atcgatcatg agcacctcag tgttggtcgc taaagagcgt
      181 cagccaggct ccccatttgg ttttgggcag aagcaagcca aaaagcaaaa aaatacggcg
      241 caaatgcgcc acagttgcgc gctattcgat gttctatcga tttttatgta atgctctgca
      301 acgagacggc gttcaacccg gtccaggctt ggctttcggg attttctgct atacgccgtt
      361 ccacaacagt tggtacggcc tcaattcatc acgctcaggc tacttgctaa gcttgcccta
      421 aacgcactct tttgctatac tgcacacaac aacagcagcg aggttttatg ataaaagaag
      481 atacacatag caggcctgtc acgccatcag gcctggaacg agcgcgcctt gcgctcgcga
      541 tctcggcagg gaaactggcg ggcgcatcgg gacgcctgct gggcgtcggc ggcgggacca
      601 gtttaccagg tttgatcgca cggcgcattg atcccaacgt actcaaatcg gtagttgggg
      661 ccagtcaagc caggaagata gtcataacgg gaagcaacgg caagaccaca actgcgcgca
      721 tgaccgccgc tatggccgta agcaacggga aacgggtctc acataaccgg gcaggctcca
      781 acatgttgca aggagtcacc gcggtggctg ttaactttgc cgatgtcttc ggacggttga
      841 acagcgacgt actgctgttt gaaatcgacg agggcacaat gcccctggtc atgccggaga
      901 ttcaacccga tgtcgtcgtg attaccaaca tctttcgtga ccagctggat cgctatggtg
      961 agctctattc ggtcgccaga gcacttgaca agacgcttga agacctaccc gaaacatcta
     1021 cgatcctcct gaacggcaac gacccacagg ttgctaactt tgggcagaag gcgaaagcca
     1081 aacgtctctt ctttggactg gaatcaaccg aggtcggaac accggttccc gaacaatcag
     1141 cggatatcat tcgctgtatc cactgccagg aagatttagt gtatgaggtt gcctacatgt
     1201 cgcacctggg catctatcgt tgtcccaatt gcggctatac gctcccgccg ttagacatcg
     1261 cggccacttc gatcaagctg gcgcctgacg gggaaggccc cacacatgta gagatgcgca
     1321 caccgcacgg caacatgacg ctggatatct cactaccagg gatacacaat gtctacaacg
     1381 ctgctgcggc catcggagct tcgctggcgg ccggttttga gaaagagcag ctaccggcag
     1441 cactcgtcga tattaagccc gcttttggac ggttggagaa gatacaggct ggcgaccaga
     1501 cgatctacct atcttttgtg aaaaacccca cgtcgtttaa cctcatactg cgcctgatcg
     1561 cccagcatcc gggtaagaag cacgtgctac tagcagcaag ccacactatt gtggatggcg
     1621 aagacttttc ctggctctgg gatatcgatg tcgaagcagt tgcgcaaggt atcgcggacg
     1681 tagtttgcag cggcaataag ccggaagaac tggcgatgcg cctgaaatac gcagagatac
     1741 ccgtggataa gatcagcctg gttgaagatc gcgaggcggc cctggatgcc gcgcttaaaa
     1801 atgcaggacc cggcggcgca ctttacattt tgagcagtta tacccccacc aacgagctgc
     1861 ggcgcattat gcagaaacgc ggttgggtta aacatacctg gcaggagtaa tttagcatga
     1921 gcgagcaagt acaagacact ggcatggtgt tgaagatcgg ccacctctac cccacgctga
     1981 tgagtgtggc ggcggaccgt ggcaacctct tttcaatcca gaagcgctgt cagtggagag
     2041 ggatcgccac cgaggtcgag cagatctatg tgcggcagac acctgatttc actaaatacg
     2101 acctgattct ttttcacggc ggggccgacc gcgagatgga gctggcttcg cgcgatatcc
     2161 aggcaaaggt gccctcgttg atcgaggctg cggagtcaaa cacggttttc ttgagcgtgt
     2221 gcgcaggttt ccaactcctt ggccattact ataaacc
//
LOCUS       DASZAK010001251         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_173794, whole genome shotgun sequence.
ACCESSION   DASZAK010001251 DASZAK010000000
VERSION     DASZAK010001251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173794"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VGT44_22010"
     CDS             complement(<1..203)
                     /locus_tag="VGT44_22010"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583551.1"
                     /translation="MVERAGQQEIVTPHNLPAQLTPLVGREQEIQTLAVLLRREDVRL
                     VTLTGPGGVGKTSLATRVAAMLL"
     gene            470..1057
                     /locus_tag="VGT44_22015"
     CDS             470..1057
                     /locus_tag="VGT44_22015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583552.1"
                     /translation="MIERQSKTSPLQQPLQSSRRPMNWRERGVGMARIIFGLVWVVDA
                     ILKWQPQFQNTFVAQVVAARDGQPAFVQAWISIWVNVVGLNPLLFARAEATTESALAI
                     FLLLGVFSNLTSVIGILISLVIWTVPEGFGGPYIPGSSTDIGTAFPYAILFVVLLCLA
                     AGRYYGLDEWLTPRLGRWGFLASSSIRRGRNRTSI"
     gene            1141..1716
                     /locus_tag="VGT44_22020"
     CDS             1141..1716
                     /locus_tag="VGT44_22020"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HEV2583553.1"
                     /translation="MIDQAGTATVGTKGTKREGFQRLAKRLGGFLMSYYAIIIAVFFA
                     MLILAAILAPILSYLGLDVIAKPIFYAMHFVCAQTPSHSFYIFGHQLCLCERCLAIYS
                     AMFLGSLVFALSKLRLPGLRLWQALLLTVPIAADGFTQMFGLRESTWELRLITGALFG
                     FAAIWYALPYAQKVLKEEAAYALAQQAKRSF"
     gene            complement(1738..2136)
                     /locus_tag="VGT44_22025"
     CDS             complement(1738..2136)
                     /locus_tag="VGT44_22025"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2583554.1"
                     /translation="MASQHVYNLMIAEDEEVERQAIQLLVERQLPRIRLVGAAATTSE
                     LLVRLNLARPHLVILDSHLPGERLVNTLNLLLSPNAALKVIIFADYDEESLMRQCLRF
                     GAFAYLARPVQPQRLLNVLKRAIVVLDNTV"
     gene            complement(2218..>2432)
                     /locus_tag="VGT44_22030"
     CDS             complement(2218..>2432)
                     /locus_tag="VGT44_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583555.1"
                     /translation="VGISREQVQKLLSDEPDNHWSAHTTGLGLRHVRDRLRHYYGEKC
                     QLVIESELGSGTTVRVLLPLDGLSGK"
BASE COUNT          520 a          703 c          653 g          556 t
ORIGIN      
        1 ctcaacagca tggcagccac ccgcgtcgcc aggctggttt taccgacgcc accgggacct
       61 gtgagggtca caaggcgcac gtcctcacgt cgcagtaaaa cagcaagcgt ctgtatctct
      121 tgctcgcgtc cgaccagggg cgtcaactgc gcgggcaagt tatgaggtgt cacgatttct
      181 tgctgtcctg ctcgctcaac catgctcaac ctcctcatgg ggatagacct gcaggtgaac
      241 cacatctact atatcgcaca caggcaatat tatcaacaca accatgcctt gtaaccccgc
      301 tgcgaatgaa cctgtgtaag aacaagctaa acaacagatc atcgagagca accactggct
      361 tctccaccgc tgaatatctc cgtaatgatt caaggacgac tcagtgatat ggtgattctt
      421 tgctcaaatt ttggtgaagc tgtacaaaaa tctcaaagga gtacattaca tgatagaacg
      481 acaatcaaaa acgtcacccc tccagcagcc gctgcaatcg tcacgccggc caatgaactg
      541 gcgcgaacga ggggtcggca tggcgcgcat catcttcggc ctggtctggg ttgtagacgc
      601 catcttgaag tggcagccac aattccaaaa tacctttgtc gcccaggttg tcgcggcaag
      661 ggatgggcaa ccggcgttcg tgcaagcctg gatatcgatc tgggtcaacg tagttggctt
      721 gaatccgctg ttgtttgccc gcgctgaggc taccacagaa agcgcgctcg ccatctttct
      781 acttctgggc gttttcagca acctgaccag cgtcattggc atcctgatat cgctcgtcat
      841 ctggacggtt cccgaaggat ttggtggtcc ctatatcccc ggctccagca ccgatatcgg
      901 cacggctttt ccatatgcta tactctttgt agtgctactc tgcctggcgg caggacgcta
      961 ttacggattg gatgagtggc tcacgccacg cctgggacgt tggggcttcc tggcttccag
     1021 ttccattcgg cgcggtcgga atcgaacgtc aatttaacct atgcaggtac acatttccgt
     1081 aggtgccgat tgatcggcac ctacggagat atctaagcgc ttgttatgag aggcatagca
     1141 atgatagatc aagcgggaac ggcaacagta gggacgaaag gcacgaagcg cgagggcttt
     1201 cagcggctgg cgaaacggct gggcggcttc ttgatgtcct attacgccat catcattgcg
     1261 gtgttcttcg ccatgttaat cctggcagct attctcgccc ccattctctc ctacctgggg
     1321 ctggatgtga ttgcgaagcc gatcttttat gccatgcact ttgtctgcgc tcagacacct
     1381 tcgcattctt tctacatttt tggacaccag ctctgtctat gcgagcgctg cctggccatc
     1441 tacagcgcca tgttcctcgg cagcctggtc tttgccttga gcaagttacg cttgccgggg
     1501 ctgcggctct ggcaggccct gctgctcacc gtgccgatcg cggcagatgg ctttacacag
     1561 atgttcggcc tgcgcgaaag cacctgggaa ttacgcctta tcaccggcgc gctcttcggt
     1621 tttgccgcga tctggtacgc actcccctat gcgcagaagg tcctcaagga agaagcagcc
     1681 tacgcgctcg cgcaacaggc aaagagatcg ttttaaccgc ctcgtaaaga tagaagatca
     1741 caccgtattg tccagcacaa caatcgcccg cttgagtacg ttcagcaggc gctgaggctg
     1801 tacgggcctg gcgagatagg caaacgcgcc gaagcgcagg cattgccgca tcagggattc
     1861 ttcatcatag tcggcaaaaa tgatgacctt gagcgcggca ttcgggctga gcagcaaatt
     1921 gagcgtgttc accaggcgct cacccggcag gtgactgtca agaatcacca gatgcgggcg
     1981 cgccaggttc aaacgcacca gcaattcgct ggtggtcgcc gccgcgccga ccagcctgat
     2041 gcggggcagt tgccgctcca ccagcagttg gatggcctgt cgctcaacct cttcatcctc
     2101 ggcgatcatt aagttgtaga cgtgttgtga agccatgttc ttagccgggg gaccgagatc
     2161 cttcgctgcg ctcaggatga cagaaccgaa gttggtgttg aaacttcatt atatcgatta
     2221 ctttcccgac aggccatcta agggcaacaa cacgcgcacg gtggtgccgc tgcccaattc
     2281 gctttcgatg accagctggc atttctcccc gtaataatga cggagacggt cgcgcacatg
     2341 ccggagtccg agaccggtgg tgtgcgcgct ccagtgattg tccggctcgt cggacagcag
     2401 cttctgcacc tgttcgcgac tgatgcccac gc
//
LOCUS       DASZAK010001252         2485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_530226, whole genome shotgun sequence.
ACCESSION   DASZAK010001252 DASZAK010000000
VERSION     DASZAK010001252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530226"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VGT44_22035"
     CDS             complement(<1..326)
                     /locus_tag="VGT44_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583556.1"
                     /translation="MMNRDRISRGRSNFGNSGTTFGLDERLERTLLYPLSALLAIFTP
                     LGWLLGLGVFLIEKNRNVRGHAAQAGVLFGTLSIAHWLVDLLGALLSHIFVVGSVLGL
                     AFGLVSL"
     gene            514..876
                     /locus_tag="VGT44_22040"
     CDS             514..876
                     /locus_tag="VGT44_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HEV2583557.1"
                     /translation="MGGLTLIIMDNQGIFLADLFGLLLSLPTALFLAFWMSAVKRKAA
                     VVIGAFVGAFLGFVVILGWVGELIYDTVLPGATSGSTFFGSVLICSAAGLVFGILADL
                     LVARLTRKDYRRPSAITE"
     gene            879..1940
                     /gene="holA"
                     /locus_tag="VGT44_22045"
     CDS             879..1940
                     /gene="holA"
                     /locus_tag="VGT44_22045"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV2583558.1"
                     /translation="MFYLVHGTDEFTCREQVKKLRRQGDFGYNSDIYIAGETPLATII
                     ATCDTFPFLTEQRLVVVEGLPKRRKGEESSASAEPARVEKEAASPNEKPEKPKKGKKS
                     AKNSTESRAGFEKGLAEYAARLPETTVLILLVDEELTASNPLLKTAQEQGKVVTCTLP
                     KGAALESWITKRAQSIGVKLTPEALSLLANYIGNQLRMLANELDKLAMYVGERGVIEA
                     DDVRKLSAQVQEARIFDLTDALAQRNRTQALDLLHDLLADGEPPLRLISTITSQVRSL
                     LLVKELAAEGLRAQQIASQTGMAPFVAEKALRQVGKFQMVQLEGAYRQLLATDAALKR
                     SRMTPEMALDLLVVNFGSL"
     gene            1937..>2485
                     /locus_tag="VGT44_22050"
     CDS             1937..>2485
                     /locus_tag="VGT44_22050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEV2583559.1"
                     /translation="MTTELQQRILERIRHEGPIPFAEYMRMALYEPGLGYYVAGAAKM
                     GWQGDYFTSADISELFAHCMGRQLLHMWEQLRRPSPFIVVEQGAGRGNLAQGVMAWAR
                     WAAPALLEALDYRIADARTGQDALAPGAALLAPSEHPSVLLSNELVDAFPVHIIELSQ
                     EQMREVYVGEAGEQAGQLCETLG"
BASE COUNT          542 a          700 c          728 g          515 t
ORIGIN      
        1 agggagacca ggccgaaggc caatcccaaa acggacccta ccacaaagat gtgagagagc
       61 agcgcgccta gcaggtcaac cagccagtgc gcgatggaga gcgtaccgaa gagcacacct
      121 gcctgcgcag cgtgtccgcg cacattacgg ttcttctcga tcaggaaaac gcccaggcca
      181 agtagccagc ccagtggcgt gaaaatggcc agcagcgcgc tcagcggata gagcaacgtc
      241 ctttccaacc gctcatcgag accgaaagtg gtgccactat tgccaaaatt tgatctgcct
      301 ctactaattc tatctctatt catcatattc ttcctcgtgc tttctgcacc ctatgagccg
      361 gacacttgct ccgctcatgt tgtatttgtg cttctgcaca catatacgca catgtttacg
      421 cgcggttgca actcttgcag gcataaaggg actgccgtat aatgagagta cactatcatc
      481 gcaaagaaaa acctttcagg aggattgacg ataatgggcg gtctcacact gatcattatg
      541 gataatcaag gcatttttct ggccgatctt ttcggcttgc tcctttcgct gccgaccgcg
      601 ctcttcctgg cgttctggat gagcgccgtc aagaggaaag ctgctgtggt gattggggcg
      661 tttgtcggcg cttttctcgg cttcgtcgtg atcctgggct gggttggtga gctaatctac
      721 gataccgtct tgcccggcgc gaccagtggc tccacgttct tcggttcggt gctgatctgc
      781 tcggctgccg gactggtgtt cggcatcctc gccgatctgc tcgtggcgcg cctgacccgc
      841 aaagattatc gcagaccatc ggccattaca gagtaagtat gttttacctg gtacatggca
      901 cagatgaatt cacctgccgc gaacaagtca agaagctgcg ccggcagggg gatttcggct
      961 ataacagtga catttatatc gccggcgaga cgcccctggc gacgattatt gccacctgtg
     1021 acacctttcc tttcctgacc gagcagcgtc tcgtcgtggt cgaagggctt cccaagaggc
     1081 gtaaaggcga ggaatcgtct gccagcgcgg aaccggcgcg cgtcgagaag gaagccgcct
     1141 cacccaacga gaagcccgaa aagccgaaaa aaggcaagaa gagcgcgaaa aactcgaccg
     1201 aatcgcgtgc tggcttcgag aaagggctgg ccgagtatgc tgcccggctg cccgaaacga
     1261 ccgtgttgat cctgctggtc gatgaagagc ttaccgccag caatccgctc ctgaaaactg
     1321 cgcaggaaca aggcaaggtt gtgacatgca cattgccgaa aggggctgcg ctcgaaagtt
     1381 ggatcacgaa acgcgcgcag agcatcggcg tcaagctgac acctgaagcg ctatcgctgc
     1441 tggcgaacta cattggcaac cagctgcgca tgctggcgaa tgaactggat aagctggcga
     1501 tgtacgttgg cgagcgaggg gttatcgagg ctgacgatgt gcgcaagctc tcggcgcagg
     1561 tgcaggaggc gcgcatcttc gatctgaccg atgccctggc gcagcgcaat cgcacccagg
     1621 cgctcgatct gctgcatgac ctgctggccg acggcgagcc acccctgcga ctcatcagca
     1681 cgatcacctc tcaggtgcgc tcgctgctgc ttgttaaaga actggcagca gaagggttgc
     1741 gcgcgcaaca aatcgcgtcg caaacaggca tggcgccctt cgtggcggaa aaagccttgc
     1801 gccaggttgg aaaatttcag atggtccagc ttgagggcgc ttatcgccag ttgctcgcca
     1861 ccgacgcggc gctcaaacgc agccgaatga cccccgaaat ggctctggat ctgctcgtcg
     1921 tcaactttgg gagcctatga ccacagaact acagcagcgc atcctggagc gtatacgcca
     1981 cgaaggacca attccctttg ccgagtatat gcgtatggct ctctatgaac cgggcctggg
     2041 ttattatgtc gcaggcgcgg caaagatggg ctggcagggc gattacttca ccagcgccga
     2101 tatcagtgaa ttgttcgcgc attgcatggg gcgtcaactg ctgcacatgt gggagcaatt
     2161 gcgtcggcct tcgccattca tcgtagtgga acagggcgcg ggacgcggaa acctggcgca
     2221 aggcgtgatg gcctgggcaa gatgggctgc ccctgcgctg ctcgaggcgc tcgactatcg
     2281 catcgctgat gcgcgcaccg ggcaggatgc acttgcaccg ggtgcagcac ttctcgcgcc
     2341 gagcgaacat ccctcggtgc tgctctcgaa tgagcttgtt gatgcttttc cggtgcatat
     2401 tattgaacta agccaggaac aaatgcgcga ggtctatgtt ggtgaggccg gagagcaggc
     2461 tggacagctt tgtgagacac tgggc
//
LOCUS       DASZAK010001253         1982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_136932, whole genome shotgun sequence.
ACCESSION   DASZAK010001253 DASZAK010000000
VERSION     DASZAK010001253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136932"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VGT44_22055"
     CDS             complement(<1..750)
                     /locus_tag="VGT44_22055"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583560.1"
                     /translation="MIKQRILSKRAGGVSLLRLSLLNMGLLDELLAGFFVIGLPLSRQ
                     QLGLSYGQIGLIFSVAALAGMVFDPVINLLSDRGSKRWWILGGLCGLVIADILAGSIA
                     TFPVLLVAWALGYTSGGAAIGLSQAALIDGAPGESTRTMTRWTMVSGVGDLLSPLAVT
                     TILAAGLGWTGLCLISAGLWLAVALVILPQRFPRPGASYSHENEEHDGGTGKSDVENL
                     YDTEAHVSILQGLRQGLRDRILLRWATLEVLT"
     gene            1208..>1982
                     /locus_tag="VGT44_22060"
     CDS             1208..>1982
                     /locus_tag="VGT44_22060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2583561.1"
                     /translation="MTQTRTPHMTDYEAERRNFHLEVPEYFNFATDVIGKWAADPQKP
                     AMLWVGPHGEERGLTFAHFADYSSRTANAFADLGIQKGDRVLVMLQRIPEWWESVLGL
                     MKIGAVPIPCTTLLTPKDIQFRAEVAEVAAFITDSEGAAKFDQVRAQCPSVKYTILVD
                     ASASDRENWTNYNRIVNEASPEFSGPKTRSDDPCLVYFTSGTVGYPKMVLHTHASYPI
                     GHTITGKYWLDLHEDDLFWNLSEMGWAKAAYSNLFGPWIM"
BASE COUNT          476 a          621 c          515 g          370 t
ORIGIN      
        1 cgtcaacact tccagagtcg cccaacgtaa caggatgcga tcgcgcaaac cctgcctcaa
       61 accctgcaaa atgctcacgt gggcttcggt gtcgtacagg ttctctacat cgctcttgcc
      121 cgtgccgcca tcatgctcct cgttttcatg gctatacgaa gctcctggcc tgggaaatct
      181 ttgcggcaaa atgacaagcg caactgccag ccataaccct gccgagatca ggcaaagtcc
      241 cgtccagccc agcccggctg ccagaatggt tgtcacggcc agaggtgaga gcaggtcgcc
      301 cacaccgctg accattgtcc agcgcgtcat ggtgcgggtg ctttcgcccg gcgcgccatc
      361 gatcaaggcc gcctgggaga ggccaatcgc cgccccgcct gacgtgtatc ccagcgccca
      421 ggccacaagt agtacaggaa aagtggcgat gctcccggcg aggatgtcag cgattaccag
      481 gccgcacagc ccacccaaaa tccaccagcg cttcgagcca cgatcactca gcagatttat
      541 caccggatca aacaccatcc ccgcaagcgc ggccacgctg aaaatcaggc cgatctgccc
      601 ataactcaac cccaactgct ggcgcgacag tggcagacca atcacaaaaa atccggcaag
      661 tagctcatcc agcagcccca tattgaggag actgaggcgc agcagcgaaa cgcctccagc
      721 acgtttcgat agaatgcgtt gctttatcat taacaggtaa tcctctctga acatgcaatc
      781 aaaacgggaa aaagttaaaa cacgttcgag agggcggcgc gcgggcatga tgaatcgcat
      841 cagcattgaa agctgacgcg caaaagcgag gattatggaa gcatcccggc catcgccggg
      901 caacaatcag ttagcaggga ctacgcgcgc gcatggatga tgctcctgag ttggactata
      961 gttctgctga tagtatacac aatattttca ataatttctt gcctcatcga gtgtcatcgt
     1021 gagcgcagcg aaggatctcg gtggccacga gccagatcct tcgctacgct cacgatgaca
     1081 ctcgagggcg gtgcgtaccc ttgcggtcgc tcttctcgct tctatcacaa agcactcgac
     1141 gcttacctcg catttgggct atactatggg caagcttacc gtcatgtatg caaaggagcg
     1201 tcgaaccatg acacagacac gcacgccgca tatgaccgat tacgaggccg aacggcgcaa
     1261 ctttcacctc gaagtccccg aatattttaa cttcgcgact gacgtgatcg gcaaatgggc
     1321 tgccgatccc caaaagcccg ccatgttgtg ggtcgggccg cacggcgaag agcgcgggct
     1381 gaccttcgcc cacttcgccg actattccag ccgtaccgct aacgccttcg ccgacctggg
     1441 catccagaaa ggcgaccgcg tgctggtcat gttgcagcgc ataccggaat ggtgggagag
     1501 cgtgctagga ctgatgaaga tcggcgcggt gcctatcccc tgcacaacgc tgctcacgcc
     1561 caaagatatc cagttccgtg cggaagtcgc cgaggtcgca gcgttcatta ccgatagcga
     1621 aggtgccgcc aaattcgacc aggtgcgcgc tcaatgcccc agtgtgaaat acaccatcct
     1681 ggtcgatgcc agcgccagtg accgcgaaaa ttggacgaac tataaccgca tcgtcaacga
     1741 ggcatccccc gaatttagcg ggccaaagac gcgcagcgac gatccctgcc tggtatattt
     1801 cacctccggc acagtcggct atcccaaaat ggtgctgcac acacacgcca gctaccccat
     1861 cggccacacc atcaccggca agtactggct tgacctccac gaagacgacc tgttttggaa
     1921 cctttcggaa atgggctggg ccaaagccgc gtacagcaac ctgttcggtc cctggatcat
     1981 gg
//
LOCUS       DASZAK010001254         1991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_600762, whole genome shotgun sequence.
ACCESSION   DASZAK010001254 DASZAK010000000
VERSION     DASZAK010001254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600762"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1113)
                     /gene="recA"
                     /locus_tag="VGT44_22065"
     CDS             complement(37..1113)
                     /gene="recA"
                     /locus_tag="VGT44_22065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV2583562.1"
                     /translation="MADRSATVVSTATTEREKAIEQALSQIERRFGKGAIMKLGDISK
                     IHVEVIPTGSIALDLALGVGGIPRGRVTEIYGPESSGKTTLCQHIIANAQRGGGYAAF
                     VDAEHALDRTYAARCGVDTNNLLISQPDTGEQALEIVDALVRSNAVDVVVIDSVAALV
                     PRAEIEGEMGDAHVGLQARLMSQAMRKLTSAISSSNTAVIFTNQLREKVGVMFGNPET
                     TSGGKALKFYASVRLDIRRTDTIKQGQDVMGNRTRVRVVKNKVAPPFRVAEFDIMYSE
                     GISREGGLIDLGLEMGLVKKSGAWFNLGEIRLGQGRENAKDYLRQNSDVAFALEEQIR
                     GNITSYKAAEVEGQADDDDEPEDE"
     gene            complement(1496..>1991)
                     /gene="recR"
                     /locus_tag="VGT44_22070"
     CDS             complement(1496..>1991)
                     /gene="recR"
                     /locus_tag="VGT44_22070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEV2583563.1"
                     /translation="SPSDQARRLAEAILRVKESIIYCSRCFNITEVDPCATCSDRTRD
                     QEIICVVEEPLDVLALEKTGYYKGLYHVLHGALSPVEGIGPKDLRIDELLQRVRNSHL
                     REIILATNPNFEGEYTANYIQKELKSFNVRVTGLARGLPMGGDLEYADEGTLGRAIEG
                     RREM"
BASE COUNT          386 a          596 c          556 g          453 t
ORIGIN      
        1 gcggcgcagg catcatatcc catcgttgag cggtcattat tcgtcttcag gctcatcatc
       61 gtcgtcggct tgaccttcga cttcggcagc tttgtagctc gtgatattac cgcgaatctg
      121 ctcttcgaga gcaaaggcga catcactgtt ctggcgcagg taatctttgg cgttttcgcg
      181 tccctggccg agccggattt cgccgaggtt gaaccaggcg ccgctcttct tgaccaggcc
      241 catttccagg ccgaggtcga tcaggccgcc ctcgcgcgaa atgccctcgc tgtacatgat
      301 gtcgaattcg gccacgcgga aaggcggcgc gaccttgttc ttgacaacgc gcacgcgcgt
      361 gcggttgccc atcacatcct ggccctgctt gatcgtatcg gtgcggcgaa tatcgaggcg
      421 aacggaggcg tagaacttga gcgctttgcc accggaagtc gtttccgggt tgccgaacat
      481 tacgccaact ttttcgcgca actggttggt gaagatcaca gcggtattgc tgctgctgat
      541 agccgaggtg agtttgcgca tagcctggct catcaggcgg gcttgcaggc ccacgtgcgc
      601 gtcgcccatt tcgccctcga tttcggcgcg tggcaccagg gcggcaaccg agtcgatcac
      661 gacgacatcg acggcattgc tgcgcaccag tgcatccacg atctcaagcg cctgctctcc
      721 tgtatcgggc tgcgagatca ggagattgtt cgtgtcaaca ccgcagcgag ccgcgtaggt
      781 gcggtcaagc gcatgttcag catcgacgaa ggctgcgtag ccgcccccac gctgcgcatt
      841 ggcaatgata tgctggcaga gcgtcgtttt acccgacgac tccgggccgt aaatctcggt
      901 gacgcgcccg cgtggtatgc cgccgacgcc cagggccagg tccagagcga ttgacccggt
      961 cgggatgacc tcaacgtgaa tcttggagat atcgcccagt ttcatgatag cgccctttcc
     1021 gaagcggcgc tcaatctgcg agagcgcctg ttcgatggcc ttctcgcgct cggtggtggc
     1081 cgtagagacg acggtcgcgc ttcgatccgc caaagacttg tcctttttgt tcatcgactc
     1141 tctcatctgc aaggggacgg cggcgtgaag gcgaccgttt gctccctgca ttcctccttt
     1201 ttcaatgctg tacctatccc cttgtagaag ggagacgttc cttttgagta attgctggca
     1261 agaaatagta aatgtgtggc tcctcaccac aagacatgcg tgtccagtaa tctattctcc
     1321 ggcagggttg cgaaaacgga aacaggctct ctcccagcct gttccttcac gatgtagaac
     1381 gattgtacca taagcacata cgtatgtcaa gaaaaacttc agcttaccca acccattaat
     1441 cgttaggcaa tccgaggaga cccgcaacag agatatcttc gtcattatac accatctaca
     1501 tctccctgcg tccctcgatg gcgcgcccca gcgtcccttc gtcggcgtat tccaggtcgc
     1561 cgcccatggg cagaccgcgc gccaggccgg taacgcgcac gttgaacgac ttcagctctt
     1621 tctggatgta gttggccgtg tactcgccct caaaattggg attggtagcg aggatgattt
     1681 cgcgcaggtg gctgttgcgc acgcgctgca gcagctcgtc gatacgcagg tctttcggtc
     1741 caataccttc cacgggtgag agcgctccat gcagcacgtg atagagtccc ttgtaatacc
     1801 cggtcttctc cagggccagc acatccagtg gctcctccac gacacaaatg atctcctggt
     1861 cacgggtcct gtcgctgcaa gtcgcgcagg gatcgacttc agtgatattg aaacagcgcg
     1921 agcagtagat aatactctcc ttcacgcgca ggatagcttc agccaggcga cgagcctggt
     1981 cggacgggct g
//
LOCUS       DASZAK010001255         1987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_315486, whole genome shotgun sequence.
ACCESSION   DASZAK010001255 DASZAK010000000
VERSION     DASZAK010001255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315486"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VGT44_22075"
     CDS             complement(<1..584)
                     /locus_tag="VGT44_22075"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV2583564.1"
                     /translation="MAQVSFVGLLIVALVAFLTPLLLGLSPARRLPSVAIEIVAGIVI
                     GPSVLGWVGIDLPISILSLIGLAFLLFLAGMEVELERLRGRLLLYVGGAFVASMGLAF
                     LVGAALFAAGQVRAPLFIAIILVATALGVVVPLLADAGESATDFGQLVIAGAMFAEFG
                     SIILLSLFFSREATGTATKVVLLIGFALLAAAFA"
     gene            complement(863..1321)
                     /locus_tag="VGT44_22080"
     CDS             complement(863..1321)
                     /locus_tag="VGT44_22080"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEV2583565.1"
                     /translation="MDTTTTNTSRTAHLLALMKQGDDAFNARDFAAVDAVHHPDMIAH
                     IPGNAEPIYGREAHAAAMRQLVRMFPDIHVYTPYPIQFGSGDWITVVTRATGTFSGEM
                     IRPDGQVIAPTGKAFDLEFGQTTKWDGDQLIVISAFYDAALLARQIGLAQ"
BASE COUNT          439 a          573 c          579 g          396 t
ORIGIN      
        1 aaggcaaagg ccgctgccag caaagcgaag ccgatcagca ggacgacctt ggtcgcggtg
       61 ccggtcgcct cacgagagaa gaacagcgac agcagaatga tcgagccgaa ctcggcaaac
      121 atggccccgg cgatgacaag ctggccaaaa tccgtggcac tttcacccgc atcggcgagc
      181 agaggtacca ccacgcccag cgccgttgct accaggatga tggcaatgaa gagcggtgct
      241 ctcacctgcc ctgctgcaaa aagggcagcg ccgaccagga acgccagccc catggaagcg
      301 acgaaggccc cacccacgta cagcagcagg cgacccctca agcgctcaag ttcgacctcc
      361 atccccgcca gaaagagcag gaaggccagg ccgatgagcg agagaatcga gatcggcaga
      421 tcgatgccca cccacccgag cacagacggg ccaatcacga tccccgccac gatctcgatc
      481 gctactgagg ggagacggcg ggccggagag aggcccagca ggagcggggt cagaaaggca
      541 accagggcaa cgatcagcaa tccgacgaag gagacctgcg ccatacgaac ctcgcaatta
      601 cagagaaggg gtcataggct taaccatatc caccaggaaa atgcggaagc gtcgtcggtg
      661 gcgaactgtt cgaacatatg cgccagtcga ctcgttgcga atgggatcgc tcggtgtcgg
      721 agtggtctga gcagtgcaag tcgtacaaca cctgtccttc tcacccaacc atgtgaggcg
      781 ggcgaacagg gcgatctttc acgatgacat cttctcatca cacccctgcg acaccgagcg
      841 gtccagttca cgactgccag gcctattggg cgagcccaat ctgccgcgcc agcaaagccg
      901 cgtcatagaa ggcggaaatg acgatgagct ggtcgccgtc ccacttcgtg gtctggccga
      961 actcgaggtc gaacgctttg cctgtcgggg cgatcacctg gccgtcgggc cggatcatct
     1021 ccccgctgaa ggtcccggtg gcgcgggtga ccacggtgat ccagtcgccg ctgccgaact
     1081 gaatggggta cggggtgtac acgtgtatgt cggggaacat gcgtaccagc tgcctcatcg
     1141 ccgcggcgtg cgcttcccgt ccgtagatcg gctcggcgtt gccggggatg tgcgcgatca
     1201 tgtcgggatg gtggactgcg tccaccgctg cgaagtcgcg ggcgttgaag gcgtcgtcgc
     1261 cctgcttcat gagcgcgagc aggtgtgcgg tccggctggt gttggtggtt gtggtgtcca
     1321 taatggttcc ctttctgaca ggttcttctc ccttgcattg agcgaggata tccgtctgat
     1381 gatacgtttc tctcttttag gtcatcgttt caaggcttcg ttggaattcc tcaatatgcg
     1441 tctgcaccat ctgttgatgc tcacgggatg cgcgaacgcg ctgccccttc agctaaagtg
     1501 gcttcacagg gccgttcaac gctctcgcgt gtcgtatgtt ctgtcctcct ttcttgttcg
     1561 ctcactgctc tctttttaca cctgggattg gtctttctgt ttgttcaggc aagcgaccac
     1621 ccgcagagcg ataggcggct ggcgtgcagc aaggcaaaat gacgttgacc tcctgctcgt
     1681 cgcgcggact atagaccatc actgcgttct ctggaaccat ccctgctaca gcgagcggaa
     1741 cgcttggttc acatcgacac aacaagtatt gacgatattt cctgtttgta caagtacgca
     1801 gttttttgtg ctatacttac aaaaaggtta ctactagaga ataagcagga ggaagcaatg
     1861 actacgcaaa agcatcggtg tgccgtagag atgctcacag gtgcgattga aggcaagtat
     1921 aaggcagtca tcttgtatca tctcatgcat ggaacgaaac gcttcaatga acttggacga
     1981 ctcattc
//
LOCUS       DASZAK010001256         5172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_104425, whole genome shotgun sequence.
ACCESSION   DASZAK010001256 DASZAK010000000
VERSION     DASZAK010001256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104425"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="VGT44_22085"
     CDS             <1..334
                     /locus_tag="VGT44_22085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583566.1"
                     /translation="VTQQVRPGTHLNAGDIAFALQVFEQVPQVVGITQFTLRYTVAGA
                     QASNGSFSIVSSDGWIAYLGNASDSNPLSNRLVELQQILKKAQQDQLTLATIDLRSGL
                     RPVFTVKP"
     gene            397..1632
                     /gene="ftsA"
                     /locus_tag="VGT44_22090"
     CDS             397..1632
                     /gene="ftsA"
                     /locus_tag="VGT44_22090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV2583567.1"
                     /translation="MQERVIASIDVGTTKICVLVGVLERDGKLNVVGVGTCPTQGLRR
                     GVVVNIEETVTSIAAALDRAERLSGKAIMSAYVGIAGSHIDSENSKGFVAISPSHRDI
                     VQNDISRAIEVARAIAIPANREVIHVIPRGYVVDGQEGIKNPIGMSGFRLEVETHIIT
                     GATSSIHNLIKCVHKARVEIEDLVLEPLASSEAVLEDGETDLGVALVDIGGGTTDIAI
                     FADGAIWQTIVLPIGGQLITSDIAIGLRLPMPVAEELKVTYGHCNPDSIAEDDMIDLS
                     HYMPDCNDLVPRKLLAEIIQARVEELFEMVHEEIKKSGYDGLLPAGLVVTGGSAVLPG
                     MLDVAGRILDLPARVGMPPNLHGLADSVSHPAYATSVGLLLWGLRHTSQYTGDEEEEQ
                     SGAGDIASRLGRWFRAFIP"
     gene            1676..3244
                     /gene="ftsZ"
                     /locus_tag="VGT44_22095"
     CDS             1676..3244
                     /gene="ftsZ"
                     /locus_tag="VGT44_22095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV2583568.1"
                     /translation="MLPLGNHQVEGFAQIRVIGVGGGGSNAVNRMILANMMGIEFIAV
                     NTDAQALLMTDAPHHIRIGDKLTRGLGAGGNPSVGAKAAEESAEEIYEALKGSDMVFI
                     TAGMGGGTGTGASPIVAQIARELGALTVGVVTKPFGFEGNKRRMAAEEGIANLKQHVD
                     TLITVPNDRLLAIADKKMPLKEAFRLADDVLRQGLLGLSDLITVPGLINLDFADVKTI
                     MSAAGSALMAIGEGIGETRAMDAAHIAIASPLLDIDINGARGVLFNITGGMDLTLYEV
                     NEAADIISKAAHPEANIIFGAVQDPAYEGKVKITVIATGFDNRAPHSQQRPAVQPNRA
                     EYNRSVYYSVPQSANGNGGMRPQQPVPQGPTYSPLPVTPPSVNISRHPTGPLAAAARP
                     AQPLQASAAMHEQARPPMPSMPMMQPLQSMQQAQPMMRGDIGDYREEDEYELNSDSAD
                     MLDEALPPLPPSRLPVPEPEAQPAQRPPHMAADSRQQRPIRRLDPKALDLPRGNRSAP
                     SGDAIDIPAFLRKR"
     gene            3404..3796
                     /locus_tag="VGT44_22100"
     CDS             3404..3796
                     /locus_tag="VGT44_22100"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEV2583569.1"
                     /translation="MEHHKGGFRSIDEYIATFPEDIQALLEAVRATIKASAPDAEERI
                     SYQMPTFALNGNLVHFAALKNHIGFYPTSSGIEAFKDELSVYEGTPGAVRFPISQPLP
                     LELISKIVHFRVTENLKKAGAKARKKKA"
     gene            complement(4017..4892)
                     /locus_tag="VGT44_22105"
     CDS             complement(4017..4892)
                     /locus_tag="VGT44_22105"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2583570.1"
                     /translation="MGLLLITRFTFQEAIRRKIFLAAVILSAVMAGVFAILLNIAVNA
                     QLSSGGGNLDPQLELLAVGIVISILVTWLVYLLSSLLTILMTTGMISSEVEAGTFSVI
                     VPKPLARAEIVFGKWLGYALILSVYIALMFFTFLGIIYWQTGYWPDQPFAAFTMLELI
                     SLSLLALTTLGSSLVPTIVNGAIVLILFIGAPIASLVQFIVQTINPTNSQALQNVTTI
                     VNLVIPTDALWHGVSYYLLPNAIGLATLNMSTNSFNTPFTSAAPIAGALLIWVILYCL
                     TLPTLAAWRFQHRDL"
     gene            complement(4903..>5172)
                     /locus_tag="VGT44_22110"
     CDS             complement(4903..>5172)
                     /locus_tag="VGT44_22110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583571.1"
                     /translation="LFDESLTLELRVSPINEELMHRLGLVALAVRQDADAPSSLLIEV
                     PNEEAAADVAAIVHECGARLYSMSPYRRSLEQLFLQTIDASRRRE"
BASE COUNT         1138 a         1452 c         1604 g          978 t
ORIGIN      
        1 agtcacacag caggtgcggc ccggcacaca cctgaacgcg ggggatatcg ccttcgccct
       61 acaggtgttc gagcaggtgc cgcaggtagt gggcatcacg cagttcacgc tgcgttacac
      121 cgtcgccggt gcccaggcgt caaatgggtc gttcagtatt gtcagttcgg atgggtggat
      181 tgcttacctg ggcaacgcca gcgacagcaa tccactcagc aaccggctgg tggagcttca
      241 gcagattttg aaaaaagcgc agcaggatca attgaccctg gcgaccatcg acctgcgctc
      301 tggtctgcga ccggtgttca ccgtgaaacc gtagagcatt cgtagtgcgg agggtagagc
      361 gtctaccctg actaacctcc ctgaggagcg tgtatcgtgc aagagcgggt gatcgccagc
      421 attgatgttg gcacaacaaa aatatgcgtc ctggtcggcg tcctggaacg cgacgggaag
      481 ctaaacgttg ttggagttgg cacctgccct acacaggggc tgcgccgtgg ggtggtggtg
      541 aacatcgaag agaccgtcac ttccattgcg gcagcgcttg accgggcaga acgcctttct
      601 ggaaaagcca tcatgtctgc ctatgtcggc atcgcgggca gtcatatcga ctcggagaat
      661 agcaaaggct ttgtcgctat ctcgccgagc catcgcgaca tcgtgcagaa cgacatcagt
      721 cgtgccatcg aggtcgcgcg cgccatcgcc attccggcga accgcgaagt cattcatgta
      781 attccgcgcg gctacgtggt ggatgggcag gaaggtatca agaacccgat cgggatgtcc
      841 ggtttccgcc tggaggttga aacgcacatc attacaggcg ctacatcgtc cattcacaac
      901 ctgattaagt gcgtgcataa ggcgcgtgtc gagatcgaag acctggtgtt ggagccgctg
      961 gcatccagcg aggcagtgct ggaggacggc gagaccgacc tgggcgtggc gctggtggat
     1021 atcggcggcg gcacgacaga tatcgctatc tttgccgatg gcgccatctg gcaaacgata
     1081 gtgctgccca tcggcggcca gctcatcacc agcgacatcg ccatcggcct gcgcctgccg
     1141 atgccggtgg ccgaggaact caaagtgacc tacggccact gcaatcccga tagcatcgcc
     1201 gaggacgata tgatcgacct gtcgcactac atgcccgatt gcaacgacct tgtgccgcgc
     1261 aaactgctcg ccgaaatcat ccaggcgcgg gtagaagagc tgtttgagat ggtacacgag
     1321 gagatcaaga agtccggcta cgatggcctg ctcccggcgg ggctggtcgt cacgggcggc
     1381 tccgccgtgc tgccggggat gctggatgtc gcggggcgca tcctggacct gcccgcgcgt
     1441 gtgggaatgc cgcccaacct gcatgggctg gccgattcgg tcagccaccc ggcctatgct
     1501 acatcggtcg ggctgctgct gtggggcttg cgccacacgt cacaatatac cggggacgag
     1561 gaagaggagc aatctggagc gggagatata gcatctcgcc tgggtcgctg gttccgcgcg
     1621 tttattccgt aggattgggc gaccgcgagg ttgcccaggc aaaggaggcc acgaaatgct
     1681 accattagga aaccatcaag ttgagggctt cgcccagatt cgcgtgattg gtgtcggcgg
     1741 cggcggcagt aatgcggtca atcgcatgat cctggcgaat atgatgggta tcgagtttat
     1801 cgccgtgaac accgatgccc aggcgttgct gatgaccgat gcgccacatc acatccgcat
     1861 tggggacaag ctgacgcggg gactgggagc aggtggcaat ccgagcgtgg gcgcgaaagc
     1921 cgccgaggag agcgccgagg agatctacga ggcgctgaaa ggctccgaca tggtcttcat
     1981 cacggcgggc atgggcggcg gcaccggcac gggggccagt cccatcgtgg cgcagatcgc
     2041 gcgcgagctg ggcgcactga cggtgggcgt cgtcaccaag ccgttcggct tcgagggcaa
     2101 taagcggcgc atggcggctg aggagggtat cgccaacctc aagcagcacg tggatacgct
     2161 gattaccgtc cccaatgatc gcttgcttgc aatcgccgat aagaagatgc cgctcaagga
     2221 agctttccgg ctggccgacg atgtgctgcg ccagggcctt ctgggcctca gcgacctcat
     2281 caccgttccc ggcctgatca acctggactt cgcggacgtg aagaccatca tgtcggcggc
     2341 aggttcggcg ctgatggcga ttggcgaggg catcggcgag acccgcgcta tggacgccgc
     2401 gcacatcgcg attgccagcc cgctgctcga catcgacatc aacggcgcgc gcggtgtgct
     2461 gttcaacatc acgggcggca tggacctgac gctttacgag gtgaacgagg cagccgatat
     2521 catcagcaag gcggctcacc cggaggccaa cattatcttc ggcgcagtac aggacccggc
     2581 ttacgagggc aaggtcaaga ttacggtgat tgccacaggc ttcgacaacc gcgctccaca
     2641 ctctcaacaa cgtccagccg tgcagcccaa tcgcgcagag tacaatagga gcgtctacta
     2701 ctcggtgccg cagtccgcta acggcaatgg gggtatgagg ccacagcagc cggtgccgca
     2761 ggggccgacc tatagcccgc tgccagtcac accgccatca gtgaacattt ccaggcaccc
     2821 gaccggcccg cttgcagcgg cagcgcgtcc cgctcagccg ctacaagctt ccgcagcgat
     2881 gcatgagcag gcccgcccgc ccatgccctc aatgcccatg atgcagccgc tgcagtcgat
     2941 gcagcaggcg caaccgatga tgcgcggtga catcggtgat tatcgggagg aagacgagta
     3001 cgagctgaac agcgattcgg cggatatgct ggacgaagcc ctgccacccc tgccaccgtc
     3061 tcgcctaccc gtgccggagc cggaggccca gcctgcgcag cgcccgccgc atatggcagc
     3121 cgactcgcgc cagcagcgcc ctattcgccg cctggacccg aaggcgctcg acctgccgcg
     3181 cggcaaccgc agcgcccctt ccggagacgc catcgatatc ccggcgtttt tgcggaagcg
     3241 gtaatagggg caacataatt acggtgtcgg atatataata gaaacgcact gaggacgttg
     3301 tcatgacagg aacaacggtt cacagcgtga acctggctgt gtgtcgatcg acgtgcatgt
     3361 cggacggttt cttctagaac aaataggagg aatgagaacg cacatggaac atcacaaagg
     3421 tggcttccgt tcaattgacg agtatattgc caccttccct gaagacatac aagcactcct
     3481 tgaagcagta cgagccacca tcaaagcctc tgcgcctgat gcggaggagc gcataagcta
     3541 tcaaatgccc acgttcgcgc tgaacgggaa tttagtccat tttgctgcgc ttaagaatca
     3601 cattgggttt tatccaacat ccagtgggat tgaggcattt aaggatgaat tatccgtgta
     3661 tgaggggaca ccaggcgctg taagattccc catcagtcag cccttgccgt tggaactgat
     3721 cagcaaaatt gttcatttta gagttaccga gaatctcaag aaggccgggg caaaagcacg
     3781 caagaaaaaa gcatgaatat cagaaaaatc tatgcagact cagcgaattc acctggacaa
     3841 tctgcaaaac agcatctcat ctttttgccc ctcagttcat tttccacaaa gctaatccca
     3901 gatattttaa catagtgttt tccctacttt ttcggcatat gaccggggac gcgggcgacg
     3961 caagcgtccc ctccccgctc cacacttccc ccgcccctac ggattatata gatcgattac
     4021 aggtcgcggt gctggaagcg ccacgcggcc agcgttggca gtgtaagaca gtagagtatt
     4081 acccagatca gcagcgctcc ggcgattggc gcagcggagg tgaagggggt gttgaagctg
     4141 ttggttgaca tgttgagggt tgccaggcca attgcattag ggagcaggta ataggagacg
     4201 ccgtgccaga gggcgtcggt cgggatgacg aggttgacga tggtggtgac gttttgcagc
     4261 gcctggctat tggtgggatt gatggtctgc acaatgaact gcacaaggct ggctatcgga
     4321 gcgccgatga agagtattag cacgattgcg ccgtttacga tggtcggcac gagcgagctg
     4381 ccgagcgtgg tgagggccag cagcgacagg gagatcagtt cgagcatcgt gaaggctgcg
     4441 aagggctggt ctggccagta gccggtttgc cagtagatga tgcccaggaa ggtgaaaaac
     4501 atcagcgcga tatagacgct caggatcagg gcatagccca gccacttgcc gaagacgatc
     4561 tcggcgcgcg caagcggttt gggaacgatt accgaaaagg tgcctgcttc gacttcgctg
     4621 gagatcatgc cggttgtcat caggatggtc agcaggctgc tcaacaggta gacgagccat
     4681 gtgaccagga tcgagatgac gatgccgacg gcgagcagtt ccagttgcgg gtcaagattc
     4741 ccgccgccac tggacaattg tgcgttgacg gcgatattga gcaggatggc gaatacgcct
     4801 gccatgacag cgctgaggat cacagctgca aggaagatct tgcggcggat ggcttcctga
     4861 aaggtaaagc gtgttatcaa taatagtccc ataatacacc ctctattcgc ggcgacgcga
     4921 ggcgtcgatg gtttgtaaga agagttgctc aagcgagcgg cgataaggcg acatgctgta
     4981 caggcgcgct ccgcactcat gtacgatggc ggcgacatcg gcggcggcct cctcgttcgg
     5041 cacttcaatc agaagggaac tgggagcatc ggcatcctgg cgcacggcca acgccaccag
     5101 gccgagccgg tgcatcaatt cctcgttgat ggggctaaca cgcagctcaa gtgtcagcga
     5161 ttcgtcgaag ag
//
LOCUS       DASZAK010001257         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_281579, whole genome shotgun sequence.
ACCESSION   DASZAK010001257 DASZAK010000000
VERSION     DASZAK010001257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281579"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VGT44_22115"
     CDS             <1..337
                     /locus_tag="VGT44_22115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEV2583572.1"
                     /translation="VASHSPREYTWERTLLLQGDVVDEIKRLKAQDGPDLQVHGSANL
                     IQTLLKHDLVDELWLKIFPLTLGTGKRLFGEGSIPAAFTLREAKTTPKGVIAASFERA
                     GDVQTGSVA"
     gene            439..1242
                     /locus_tag="VGT44_22120"
     CDS             439..1242
                     /locus_tag="VGT44_22120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007921791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2583573.1"
                     /translation="MPTVTSKDGTKIAFDRVGSGPAVILVSGATAYRAFDPSMGQLAE
                     LLGKHFTVYNYDRRGRGASSDTRPFAKEREIEDLQALVEDAGGTAMIFGISSGAVVSL
                     DATAVTPGITRIAVYEPSLIVDASRQPVPADYAEHLMRLSTEGKRDEAVAYFLTQAVG
                     IPAEYIGGMKQDQAMWSGVTGVAHTLAYDAAFVGDVMQGKPLPPDRWVKVTVPVLVVD
                     GGASDAWIHHGADALANVLPHASRQTLAGQTHMVDPNMLAPILIEFFQQ"
     gene            1378..1737
                     /locus_tag="VGT44_22125"
     CDS             1378..1737
                     /locus_tag="VGT44_22125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583574.1"
                     /translation="MTLLVVLNSTVCVKDRLTGFEGQSTQPRALPPDIADFPGFAARL
                     PAISQVSTMPACNGPVSYRGQAEVARDLANLADALEGVQVAEAFLTAASPGVIALFLE
                     NQYYPTHEAYLSALVRQ"
     gene            complement(1710..>2011)
                     /locus_tag="VGT44_22130"
     CDS             complement(1710..>2011)
                     /locus_tag="VGT44_22130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583575.1"
                     /translation="EGEGFWAGLQRIGQDRPQGNITVVRPLVVAPTEMQPQAIRRDIP
                     HRLVECLDVQGAVVLAPVLTAYPADAEIGTIQLQEKAGFDDGVVFLLHCLTSAER"
BASE COUNT          402 a          605 c          608 g          396 t
ORIGIN      
        1 tgttgcctcg cattcgccga gggagtatac gtgggagcgt accctgctgt tgcagggtga
       61 cgttgtcgat gagatcaaaa ggctgaaagc gcaggatggg cctgatctgc aggtgcatgg
      121 tagcgccaac ctcatccaga cgcttttgaa gcacgacctg gtggacgagc tgtggctgaa
      181 aatcttcccg ctcacgctgg gcaccggcaa gcgattgttt ggcgaaggta gcattccagc
      241 agcatttacc ctgcgcgaag cgaaaactac gccgaaaggc gtcattgctg cctcatttga
      301 gcgcgcggga gacgtacaaa cggggtcggt tgcctgatcg ttcaccttga agtaaaacgc
      361 ccgaagtgaa gcttgcgcat ggacgcagcc ttcgcttcgg gtgtgatgtt catgaccatc
      421 tgcaaagagg agaatcctat gccaacagtc acttctaaag acggcacgaa aattgctttc
      481 gacagggttg ggtccggccc agctgtcatc ctggtcagcg gtgcgacggc gtatcgtgcc
      541 tttgacccga gtatgggaca actggccgaa ctgcttggaa aacacttcac ggtctacaac
      601 tacgaccggc gcggtcgtgg cgcgagctcc gatacgcggc ctttcgcgaa ggaacgcgag
      661 attgaagacc tgcaagcgct ggtcgaggac gccggcggca cagctatgat ctttggcatt
      721 tcgtcaggcg ctgtcgtgag cctggatgcc actgctgtca cgcctggtat taccagaatc
      781 gcggtgtatg agccgagtct cattgtcgat gccagccgcc agcccgtgcc tgccgactac
      841 gcggagcatc tcatgcggct ttcgacagag gggaagcgcg acgaagcggt cgcatacttt
      901 ttgacgcaag ccgtgggcat tccggcagaa tacatcggtg gcatgaagca ggatcaagca
      961 atgtggtccg gcgtgaccgg cgtggcacat actcttgcct acgacgcggc gtttgtcggc
     1021 gatgtcatgc agggcaaacc gctcccacct gatcgctggg tcaaggtgac tgtgccggtg
     1081 ctggtggttg atggcggtgc gagcgacgcc tggatacacc acggcgctga tgcgctggcc
     1141 aacgtcctgc cacacgccag ccgccagacg ctcgcaggtc aaacccatat ggttgacccc
     1201 aacatgctcg ccccgatcct catcgaattc tttcagcaat agcaccggat gtcaatccca
     1261 gaactctgtg ggaaaattta ggacaggtac ctgggtaggc aagcagtctg aaaagagggg
     1321 ctttcggcca ggacccgccc gcagaattat acgaatttct ctcacagagt tctgggattg
     1381 acacttctgg ttgttctcaa ctccaccgtt tgtgtcaagg accgcctgac cggctttgaa
     1441 ggacagagca cgcagccgag agccctcccg ccggatatcg ccgactttcc aggcttcgcc
     1501 gcgcggctgc ccgcgatcag ccaggtctcg accatgcccg cctgcaatgg cccggtgagc
     1561 tatcgcgggc aggcggaggt cgcgcgcgac ctcgccaacc tggccgatgc cctggaaggc
     1621 gttcaggtcg ccgaggcctt tctgaccgcc gcttcgccgg gcgtcatcgc gctgttcctg
     1681 gagaaccagt actacccgac ccacgaagcc tatctctccg cgctggtcag gcaatgaagg
     1741 aggaatacga caccatcgtc gaagccggct ttctcctgca actggattgt cccgatctcg
     1801 gcgtcagccg ggtacgcggt gaggactggc gcgaggacta ccgcgccctg cacatccagg
     1861 cactcaacca ggcggtgcgg gatatcccgc cggatcgcct gcggctgcat ctctgttggg
     1921 gcaactacga ggggccgcac caccgtgata ttcccctgcg gacgatcctg cccgatgcgc
     1981 tggaggcccg cccagaagcc ctctccttcg a
//
LOCUS       DASZAK010001258         2231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_104431, whole genome shotgun sequence.
ACCESSION   DASZAK010001258 DASZAK010000000
VERSION     DASZAK010001258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104431"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..>2231)
                     /gene="recQ"
                     /locus_tag="VGT44_22135"
     CDS             complement(81..>2231)
                     /gene="recQ"
                     /locus_tag="VGT44_22135"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEV2583576.1"
                     /translation="DNIEQALKQHFGHESFRAGQREIIEYALRGYDAFVLMPTGGGKS
                     LVYQLPALLMPGLTVVISPLIALMQDQVDRMRANGIPATFVNSSLSSREQFERERDAL
                     HGKIKLLYVAPERLVSGNSLSFLDQVDRTVGLSLLAVDEAHCVSEWGHDFRPEYRRLG
                     LLRARYPHVPVMALTATATERVRQDILEQLRLRDPHIHIASFNRPNLYYEVRQKNKGS
                     FGELVQLLREQPDAPVIIYCQSRKSVDELSEALRQNGIRALSYHAGMTSEQRREHQSR
                     FIRDDVSVLVATIAFGMGIGKPDVRAVIHYDLPRNLEGYYQESGRAGRDGQTAQCIIF
                     FNHGDRVKVEFMIEQKPDEQEQRIARQQLQQVVAYGESSACRRRVLLGYFGETLSGEK
                     CGNCDNCLRPSTMEDRTGDAQKFLLCVAKTGQRFGIRQVIDVLRGANTQKIRDYRHDQ
                     LPIYGKGKELSADEWQRIGRALLHQGLLTETTDGYPVLRLNALSLEVLRKERAVEIAA
                     PARPVQKRQARSGDPSEMTAGEEELFQRLRVLRKRIADEQGVAPYVVFPDTSLRAMAQ
                     RRPQSRSQFALIPGVGSSKLDAYCEAFTNEIRDHRLEIAPQRTSNVAPVNGGITHQTT
                     LALYRQGLSIEEIASERNLKPSTIIGHLSELFEAGEAIDIDRLVPPEHFPVIADALQQ
                     IGDDLLRPVKDFLGDEYSYEEIRLVRASLRRTYE"
BASE COUNT          496 a          634 c          562 g          539 t
ORIGIN      
        1 cgccacaata ataccagaga tttatttcag tattaaatga gcaaggatgc tgaaacagaa
       61 accacggaag aagttttgta tcattcataa gtccggcgta gtgacgccct gaccagtctg
      121 atctcttcat aagaatattc atcgcccaga aaatccttga ccggcctcag aaggtcatca
      181 ccaatttgtt gcagcgcatc tgctatcacc gggaaatgtt ccggcggcac cagcctatca
      241 atatctatcg cttcgcctgc ttcgaatagc tcagaaaggt ggccaataat agtgctgggt
      301 ttgaggttgc gctcgcttgc aatctcctca atactgagcc cctggcgata caacgccagc
      361 gtcgtttgat gagtgatgcc gccattcaca ggagccacat tcgatgttct ttgtggtgca
      421 atctccaggc ggtgatcccg aatctcgttc gtgaaagcct cgcaatacgc atccagttta
      481 ctactgccga ctcctggaat cagcgcgaac tgcgagcggc tttgtggcct tcgttgagcc
      541 atcgcgcgta aacttgtatc agggaagaca acatacggag ccacaccttg ttcatccgcg
      601 atccgtttgc gtaacacacg cagacgctgg aatagttcct cctcaccggc tgtcatctca
      661 gatggatcac cgctcctggc ctggcgcttt tgtacaggtc tggcgggcgc ggcaatctcg
      721 actgcgcgct cttttctcag gacttccagg gaaagcgcat tcaacctaag caccgggtag
      781 ccatcggttg tctcggtcaa aagcccctgg tgaaggagtg cgcggccgat tcgctgccat
      841 tcatcggcgc tcagctcctt ccctttgccg taaatcggca attgatcatg acgataatcc
      901 cgtatcttct gcgtgtttgc tccgcgcaac acgtcgataa cctggcgaat gccgaaacgt
      961 tgtcctgttt ttgcaacaca taataaaaat ttctgggcat cgccggtccg gtcttccatg
     1021 gttgacgggc gcaggcagtt gtcgcagttg ccacatttct caccactcaa tgtttcaccg
     1081 aagtagccca gcagcacacg acggcgacag gcgctgcttt caccataagc taccacctgc
     1141 tgaagctgct ggcgagcgat gcgctgctcc tgctcgtcag gtttttgttc gatcataaac
     1201 tcaaccttga cccggtcacc atggttgaag aaaatgatgc actgcgcggt ctgtccatcc
     1261 cgacccgccc ggcctgattc ctggtaatag ccctctagat tgcgcggcag gtcgtaatgg
     1321 atgactgcgc gtacatctgg tttgcctatg cccatgccga aggcgatggt tgccactaaa
     1381 accgatacat cgtcacgaat gaagcgcgac tggtgctcgc ggcgttgctc gctggtcatg
     1441 cctgcatgat atgagagcgc gcgaatgccg ttttgtctca gtgcctcgct caattcatcg
     1501 acacttttgc gactctggca ataaataatg accggcgcgt caggctgctc gcgcagcagt
     1561 tgcactaact caccaaacga ccccttgttt ttttgtcgta cttcatagta gaggttgggc
     1621 cggttgaaac ttgcaatgtg aatatgaggg tcgcgcagcc gcaattgttc caggatatcc
     1681 tggcgaactc gttcggttgc cgttgcggtc agagccatta ccggtacgtg tggatagcgc
     1741 gcgcgcaata gccccaggcg ccggtattcc gggcgaaaat cgtgtcccca ttcactcaca
     1801 cagtgcgctt cgtctactgc gagcagcgat aaaccaactg tacgatcaac ctgatctaaa
     1861 aacgagaggg aattcccgct taccagtcgc tctggagcca cgtaaagcaa ctttattttg
     1921 ccatgcaagg catcgcgttc tcgctcaaat tgttcgcggg aactcagcga actgttgacg
     1981 aaggtggcgg ggatgccgtt ggctctcatg cgatctacct ggtcctgcat cagcgcaatc
     2041 agcggcgaga tgaccacggt tagcccgggc atcaataagg ctggaagctg gtagacgagc
     2101 gacttccccc cgccggtggg cattaaaacg aatgcatcgt atcctctcaa tgcatactca
     2161 ataatctcac gctgtccagc ccgaaacgac tcgtgcccga aatgctgctt aagcgcctgc
     2221 tctatgttat c
//
LOCUS       DASZAK010001259         2065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_564175, whole genome shotgun sequence.
ACCESSION   DASZAK010001259 DASZAK010000000
VERSION     DASZAK010001259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564175"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VGT44_22140"
     CDS             complement(<1..1066)
                     /locus_tag="VGT44_22140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583577.1"
                     /translation="MSMPDKWEYPWYASWDLAFHCLPLALVDSTFAKSQLDLLLREWY
                     MHPNGQLPAFEWSFDDVNPPVIAWAAWQVYTIDREQAGQADRMFLESIFHKLLLNFTW
                     WVNRKDSEGKNVFQGGFLGLDNIGVFDRSAPLPTGGHLEQSDSTSWMGMFCLNMMTIA
                     LELALANPVYEDIALKFFEHFLAIAAAMNNIADEGIHLWDEQDGFFYDVLNRPDGTRL
                     SLKIRSLVGLIPLCAVEILEPETLQALPRFNYHLTWFLTHRPELARLVHHLQPSEQPG
                     AGERHLLALAQGDRVKRLLRHMLAPDEFLGDYGIRSLSKFHLDHPYILNVDGTAHTVS
                     YEPAESRTGLFGGNSNWRGPI"
     gene            complement(1066..>2065)
                     /locus_tag="VGT44_22145"
     CDS             complement(1066..>2065)
                     /locus_tag="VGT44_22145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583578.1"
                     /translation="SRAYRWNEDGLAGISDDKGNLCFAIALWNGQDAILKERLFGLTG
                     LQGNHGEDVKEVYYYLDNTPTHSYMRMLYQYPQAAFPYQELIEENALRGRNAPEFELT
                     DTGIFANGRYFDIFVEYAKAAPDDLLIRVNAVNRGPEAAILHVLPSLWFRNTWSWGLD
                     DHMPIVRQGTGNLPDNPSIEAEHHTLGNYRLYCEAADDLLFTENVTNARRLFGAPNAT
                     PYVKDAFHDFLVRGNREAVNPARVGTKAAALYTRTIAAGEMLTLRLRLTRVSSDENTV
                     TGYAPDDQLRTAHADPFADYDAVFDLRKQEADEFYAALQPADLDATPAGDISITSA"
BASE COUNT          389 a          557 c          689 g          430 t
ORIGIN      
        1 agatggggcc gcgccagttg gagttgccgc cgaagagtcc ggtgcgcgac tccgccggtt
       61 cgtaggagac agtatgcgcg gtgccatcga cgttgaggat gtagggatga tcgaggtgga
      121 atttgctgag cgagcggatg ccgtagtcgc ccaggaattc gtcgggggcg agcatgtggc
      181 ggagcagacg tttcacgcgg tcgccctgtg cgagcgccag caggtgacgc tctcctgcgc
      241 cgggctgttc tgacggttgc aggtgatgta ccaggcgggc cagttctggc cggtgggtca
      301 ggaaccaggt caggtgatag ttaaagcgcg gcaaagcctg gagcgtctcc ggttcgagta
      361 tctcgaccgc gcagagggga ataagtccga cgagcgagcg gattttgagg gacaggcgcg
      421 taccgtcggg gcggttgagc acgtcgtaga aaaacccatc ttgctcgtcc cagaggtgaa
      481 tgccctcgtc ggcgatgttg ttcatggcgg cggcaattgc caggaaatgc tcgaagaact
      541 tcagcgcgat atcttcgtag acggggttgg ccagcgcgag ttccagggca atcgtcatca
      601 tgttgaggca aaacatgccc atccagctcg tgctgtcgct ctgttcgaga tgtcccccgg
      661 tgggcagcgg tgcgctgcgg tcgaatactc ctatgttgtc cagccccaga aagccgccct
      721 ggaagacgtt cttgccctcg gagtccttgc ggttgaccca ccaggtgaag ttgagcagga
      781 gtttgtggaa gatgctctcc aggaacatac ggtccgcctg ccctgcctgc tcgcggtcga
      841 tggtgtagac ctgccaggcg gcccaggcga ttacgggcgg gttcacatcg tcgaatgacc
      901 attcaaaggc gggcaactgg ccgttggggt gcatgtacca ctcccgcagg agcaggtcga
      961 gctggctctt ggcgaaggtg ctgtcgacca gcgcgagcgg caggcagtgg aaggccaggt
     1021 cccaggaggc gtaccaggga tactcccact tgtccggcat ggacatcacg cgctcgttat
     1081 agagatgtcg ccagccggtg ttgcgtccag gtcggcgggc tgcaaggcgg cgtagaattc
     1141 atcggcctcc tgcttgcgca ggtcaaagac ggcgtcatag tctgcgaatg ggtccgcgtg
     1201 ggctgtgcgc agttggtcat caggtgcata tcctgtcaca gtgttttcgt cgcttgatac
     1261 cctggtcagg cgcaggcgta aggtcagcat ttccccggcg gcaatagttc ttgtataaag
     1321 ggcggcagcc ttcgttccta cgcgcgcggg attgaccgcc tcgcgattgc cacgcacgag
     1381 aaaatcgtgg aaggcatctt tcacatacgg cgtggcgttc ggcgcgccga agagtcgccg
     1441 ggcgttcgtt acattctcgg tgaagagcag atcatcagcc gcttcgcagt ataggcggta
     1501 gttgccgagg gtgtgatgct ccgcttcgat ggaggggttg tcgggaaggt tgcctgtccc
     1561 ctgccgcaca atcggcatat gatcgtccag gccccacgac caggtattgc gaaaccacag
     1621 gctcggcagc acatgcaata tggccgcctc tggaccgcga ttgacggcgt tgacgcggat
     1681 caacaggtcg tcaggggctg ctttggcata ttcaacgaag atatcgaagt aacgcccatt
     1741 cgcaaagatg ccggtatccg ttaactcgaa ctcaggggca ttcctgccgc gcagggcgtt
     1801 ctcttcgatc agctcctggt aagggaaagc cgcctgggga tactggtaca gcatgcgcat
     1861 gtagctgtga gtgggggtgt tgtcgaggta gtagtagact tctttgacat cttcgccatg
     1921 attgccctgc aggcccgtca gaccaaacag gcgctctttg aggatggcat cctggccatt
     1981 ccagagggcg atggcgaagc acaggttgcc tttgtcatcg ctgatgccag ccaggccgtc
     2041 ttcgttccag cggtaggcgc gcgaa
//
LOCUS       DASZAK010001260         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_707552, whole genome shotgun sequence.
ACCESSION   DASZAK010001260 DASZAK010000000
VERSION     DASZAK010001260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707552"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2681
                     /locus_tag="VGT44_22150"
     CDS             <1..2681
                     /locus_tag="VGT44_22150"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2583579.1"
                     /translation="NIPPMPPLPSLDPEQEKRRRFEILADFLTAQAINKPVFLIVEDL
                     HWSDDTSLEFLHYLARRCAAHPLLLLLTYRSDEVSPDLRHFLAQLDRERLVQEISLAR
                     LARDEVEAMLRAIFELSRSARLELPDPIYALTEGNPFFVEETLKSLIAAGEIYYANGR
                     WDRKPLGELHIPRSVQDAVRQRADQLSEAARRVMTLAAVAGRRFDFGLLQQLTPYDEQ
                     QLLVLMKELIAAQLVIEESAEQFAFRHALTRQAIYADLLVRERKALHRSIADLMEHNY
                     SSTLDTHVADLAYHFYEAGAWEKALAYGQRAGEQAYRLYAPHVTVEQVTRALDAAQRG
                     SIPPPVSLYRLRGRAYETLGDFERARLDDETNLRMIQGAGDSPAEWQALCDLGFLWAG
                     RDYSQAGTYFQQALSLARQMDDPLTLAHSLNRLGNWHLNIEQPRDALHYHQEALTLFQ
                     QARDQHGLAETFDLLGMTATLGGDLLQGTTYYQQAVALFQELDDRQGLASSLATLAVL
                     GGEYETETMVPAPVSFAECLRLAEQAIKIAREIGQRSAEIYALLALGQYLGPRGEYVY
                     ALQVTQEGLLLAEKIEHHEWMTNGYWVLGALYFDLLALPEARISLEHALALAHEVGSW
                     NWIRIVSGFLARVLVLQQDLTKAESTLTAALEPDAGMQTIGQRLVWAARAELALARGD
                     PGQALDITDRLIASATNLSDKHVIPHLWKLRGEALSALDRLMEAEIALRAAQEAAYAQ
                     GLRPLLWRVYVALGRLYQTQGRQEETEQAFATARTIIEELAAPILDETLRASFLSAAR
                     LRLPRSRPLTPRRATNQAFSGLTEREREVAILLAEGKSNREIAQRLVVGTRTVEVHVS
                     NILSKLGFTSRAQIAVWAYEKGLASTPNQGLFGGDR"
     gene            2948..>3839
                     /locus_tag="VGT44_22155"
     CDS             2948..>3839
                     /locus_tag="VGT44_22155"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2583580.1"
                     /translation="MTTQQTIDQAKADAFVGKVLSDTAALAVTVMSSIGDRLGLFKNL
                     AQQGPATSEELAERTHINERYAREWLSAMASAGYLEYDPVRRRFTLPPEHVPVLATEG
                     GPVFFGGVQEEVVGLAGPVNQLMQAFRTGGGVPMEAYDSSTWEGVTRFTSGWFENLLV
                     PVWLPAMPGVQAKLERGALVADVGCGHGKALIKLAQTYPQSHYVGYDSFAPSIEQAKT
                     NAQAAGVGDRVRFEQRDVSEGLPEQYAVITTFDVVHDAINPRGLLRAIRNGLRPGGRS
                     VCLDINSSDKLEENIGLLGAF"
BASE COUNT          802 a         1116 c         1121 g          800 t
ORIGIN      
        1 caaatatacc tcctatgcct ccactgccat cgcttgatcc cgagcaggag aagcgccgcc
       61 gctttgagat cctggcagat ttccttacgg cacaagcgat caataaacct gttttcttga
      121 ttgtggagga cctccattgg agtgatgaca ccagcctgga atttctccac tacctggcac
      181 gacgctgcgc tgctcatccg ctccttctcc tgttgaccta ccgcagcgac gaggtgagcc
      241 ccgacttgcg tcatttcctg gcgcaattgg atcgagaacg actggttcag gagatctcgc
      301 tcgcccgcct cgcacgagat gaggtcgagg cgatgctgcg tgccatcttc gagctctcgc
      361 gctctgcccg gttagaactt cctgatccca tctatgcact tacggagggc aatcctttct
      421 tcgttgaaga aacgctgaag tcactgattg cggcaggaga gatttattac gccaatggcc
      481 gctgggatcg caagcctctg ggcgagttgc acatcccgcg cagcgtccag gacgccgtgc
      541 gccagcgtgc agatcaactg agcgaggctg ccaggcgggt aatgaccctg gcagcagtcg
      601 ccgggcgacg cttcgatttt ggcttattgc agcagctaac accatacgac gagcagcaac
      661 tgcttgtgct tatgaaagag ttgatcgccg ctcaattagt catcgaagaa tcggcggagc
      721 agttcgcctt ccgccacgcg ctgacacgac aggccatcta tgccgacctg ctggtacgcg
      781 aacgcaaggc gctacatcgc agcattgccg acttaatgga acacaactac tcctcgacgc
      841 tcgatacaca cgtggcggac ctggcctacc atttctatga ggcaggagcg tgggagaaag
      901 cgttggcata tgggcagcgc gccggggagc aagcatatcg cctgtacgct ccgcatgtca
      961 ccgtcgagca ggtgacgcga gccctggatg cggcacagcg tggatcgatt ccacccccgg
     1021 tctcgctcta tcgcctacgg gggcgagcct acgagaccct cggtgatttt gagcgggcac
     1081 gcctggatga tgagaccaat cttcggatga ttcagggagc cggagacagc ccggcggaat
     1141 ggcaggcctt gtgtgactta ggttttctat gggcaggacg cgattactcc caggcaggga
     1201 cctattttca gcaggcgctt tcgttggcgc gccagatgga tgatccgctc accctggctc
     1261 atagcctcaa tcgcctgggc aactggcacc tgaatatcga gcagccccgc gatgcgctgc
     1321 actaccacca ggaggcgctc acgctgtttc agcaggcgcg tgatcagcat ggactcgccg
     1381 agacattcga cctgctgggc atgacagcta ctcttggcgg tgatcttcta cagggtacta
     1441 cctattacca gcaagctgtt gcgctcttcc aggaactcga cgaccgtcaa gggttagcct
     1501 ccagcctggc gacgttggcc gttttaggcg gtgagtatga aaccgagacg atggtccccg
     1561 ccccagtcag tttcgccgag tgcctccgcc ttgctgagca agccatcaag attgcgcgtg
     1621 agattggcca gcgctctgcc gagatatacg cgctcctggc cttggggcaa tacctgggtc
     1681 cgcgcggcga gtacgtctat gccttgcagg tgacacaaga gggtctctta cttgcagaga
     1741 agatcgagca tcacgagtgg atgactaatg gatactgggt gctaggagcg ctgtatttcg
     1801 acctcctcgc gctccccgag gcgcgaatct cgctcgaaca cgcgctcgcg ctcgcccacg
     1861 aagttggctc gtggaactgg attcgtattg tctcgggatt ccttgcccgg gtgctcgtcc
     1921 ttcaacaaga cctcactaag gccgaatcga ccctcactgc cgcgcttgaa cctgatgccg
     1981 gtatgcaaac catcgggcag cggctcgtct gggctgcacg cgctgaactc gccctggcac
     2041 gaggtgatcc tggacaggcg ttggacatta ctgaccggct gatcgcatct gccaccaatc
     2101 tgtccgacaa gcatgtcatc ccccacctct ggaaactgcg aggtgaggcg ctgtccgcgc
     2161 tcgacagact gatggaagcc gagattgcgc tacgagccgc gcaagaagct gcatatgcgc
     2221 aggggctgcg accgttgctc tggcgcgtgt acgttgcact gggaagactt tatcaaactc
     2281 aggggcgcca ggaagagacc gagcaggcat ttgccaccgc aaggacaatc atcgaggagc
     2341 ttgctgctcc tatcctggat gagacacttc gagcatcgtt cctgtcagca gcgcggctgc
     2401 ggctgccgcg ttcgcgccca ctcacgccgc gtcgtgcaac caaccaggct tttagtggcc
     2461 tgaccgagcg cgaacgcgag gtcgccatcc tgcttgcgga aggcaaatcg aatcgggaga
     2521 tcgcgcaacg gttggtggtt ggcactcgaa ccgtggaggt gcatgtcagc aacatcttat
     2581 cgaagttagg gtttacctcg cgcgcgcaaa tagcggtctg ggcttatgaa aaaggcctgg
     2641 cgagcacgcc gaatcagggc ttgttcgggg gagatcgctg agcgctgatc gcctcgtccg
     2701 tctatcgctc taccaatccc caggtatgaa cgtattctct ccgctaatca atcccgatac
     2761 tttacgtaaa tttctgcccc atctcttacc tctcttcgca aatactacgt agtttttgcg
     2821 caaaaaattt cggttttccc cactggaaag tcagcctttt tcgggcatac tactggtgac
     2881 ggttggaatc agaccagtgt caaccgcaca aataagcagt attgctgcag aaaaaggaga
     2941 aaaagacatg acaacgcaac agactatcga tcaggcaaag gccgatgcat tcgtgggtaa
     3001 ggtgctgtca gacacagccg cacttgccgt aacggtgatg tcgagcatag gtgatcgcct
     3061 cggcctcttc aaaaatctgg cgcagcaggg accggccacg agtgaagaac tggcagagcg
     3121 cacgcacatc aacgaacgct atgcacgcga gtggctcagc gccatggcca gtgccggtta
     3181 tttggagtac gacccggtac gtcgtcgctt caccctcccg cctgaacacg ttccagtgct
     3241 tgccacggaa ggtggacctg tcttcttcgg cggggtacaa gaggaagtag tgggattggc
     3301 cggaccggtc aaccagttga tgcaagcctt ccgcaccggt gggggcgtcc cgatggaggc
     3361 ttacgactcc agcacctggg aaggcgtgac acgctttacc agcggctggt ttgaaaattt
     3421 gttggtgccg gtgtggctgc cagccatgcc aggggtgcaa gccaaactgg aacgcggtgc
     3481 tctggtggct gatgtgggat gtgggcacgg aaaggccctg atcaagctgg cccagaccta
     3541 tccacagtca cactacgttg gctatgacag cttcgctcca tcgattgaac aggcaaagac
     3601 taatgcccag gcagcaggcg tgggagatcg cgtgcgcttc gagcagcggg atgtgtcaga
     3661 gggactgcct gagcagtacg ctgtgatcac caccttcgat gtcgtgcatg acgcgatcaa
     3721 tccacgcggg ctgctgcgtg ccatccgcaa tggactgcgg cccggtgggc gctctgtgtg
     3781 cctggacatc aacagctcgg acaaactgga ggaaaacatc gggctgcttg gtgcgtttt
//
LOCUS       DASZAK010001261         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_137072, whole genome shotgun sequence.
ACCESSION   DASZAK010001261 DASZAK010000000
VERSION     DASZAK010001261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137072"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1331
                     /locus_tag="VGT44_22160"
     CDS             <1..1331
                     /locus_tag="VGT44_22160"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2583581.1"
                     /translation="SGDPGGRHATPRRTHQSVLCFVMFFLACPVVACSAPATTQTVVR
                     RATPTVVMAGGEAVLPHSQIFYHDAPLPTLADFQVGADAWTLAGYDSDGTRSVAMPAC
                     CSTDSALPLWFAASSAPLLYAPIIGNGHIYLLAADGYLHVLNQQDGSEAWRVAVGGDL
                     TSNGLALAHGLLYVALDGHYIAALDATTGQERWRFDTGGVVRAAPLVVGHILLVESGP
                     NTLWCLDALTSEKYWVFHSEDALAQFWPSRTPPVIASGLVYVALGAANEFNALNLRTG
                     RKVWESSVPERMTGGPVLDQGLGLVYVLTWSGHLLAYDAHSGALRWKIALPGGSESSP
                     ALDEQTHTLYLGSFDGSLYAIDARTGHVQWRLATGSPVTASPLVVQGATQSRVIAATQ
                     AGLCIMVDARSGRRLNTWNLGELRAAPVMAQGILYLASLGKQGLFALELQ"
     gene            1352..2827
                     /locus_tag="VGT44_22165"
     CDS             1352..2827
                     /locus_tag="VGT44_22165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583582.1"
                     /translation="MGRKLSFPRKVLLLLLLIPLGVGLTYAWQYLHPPAPKPRPASPP
                     VLTYKPVVGDVNDPEEDDPDIAIQQSLIPGFQFKQGGYVLSGIARDARTGQPVADAVV
                     WIGLPARAGQPTSPALHTVADAGGNFQFNHLATDVYTLVASRYYNIGDNRYYAERVFS
                     HVTLQGNRSGLVLSLTSIPAPGRRSIGTGRPQNLIVIDLRGFYADSLLNDPLLVNETP
                     NLRAFLRYSTTLTSLWQPYGWRPPDQYALLTGSYPRWATYDTWPNPVPWGVPDGIDTQ
                     FWFTGGRSAHLFGQESIFDVAKGYGMQTGVVAGSDFVLSDATTRNLDLLQQDSAFAPR
                     NWLAQMQDAVLSGAQQANGFMLYGELAPLSADDANASPDASGDAYQQALLRADQTIGQ
                     LLAWLGQQGLLQHTLIALTTSQAQANHTDADNYYGMGITGQGTSKQTMLALSWPGGCI
                     GRGGTSDTTVYSSFVIAPILMRVMGLPAPAEARLSAPGGCI"
BASE COUNT          524 a          917 c          894 g          592 t
ORIGIN      
        1 gtagtggcga ccctggcggt cgccatgcca caccacgccg cacacaccag tccgttctct
       61 gttttgtaat gttttttctc gcctgcccgg tcgtcgcttg cagcgcgcct gcgaccacac
      121 agacggtggt tcgacgtgcg acgccgacgg ttgttatggc tggcggcgag gcggtgctgc
      181 cgcattcgca gatcttttac cacgacgcgc cgctgcccac actggcggat ttccaggttg
      241 gggctgatgc ctggacgctt gccgggtatg atagcgatgg gacgcgctcg gtggcgatgc
      301 ccgcgtgttg cagcacagat agcgcgctcc cgctctggtt cgcggcatca agcgcgccgc
      361 ttttgtacgc gccaatcatc ggcaacggcc atatctacct tctggccgcc gatggctacc
      421 tgcacgtact gaaccagcag gatggttccg aggcgtggcg cgtggctgtc ggaggcgatt
      481 tgacctcgaa cggcctggcg ctcgcgcatg gcctgctcta cgtggcgctg gacggtcact
      541 acatcgcggc gctggatgca accaccggcc aggagcgctg gaggtttgat accgggggcg
      601 tggtacgcgc cgcgccgctg gtcgtcgggc atatcttgct cgtcgaaagc ggtcccaata
      661 ccctgtggtg tctcgatgcc ctgacgagcg aaaaatattg ggtcttccat tccgaggatg
      721 ccctggcgca gttctggccg tcgcgcactc cacccgtcat cgcgagcggg ctggtctacg
      781 ttgcgctggg cgctgccaac gagttcaacg cgctgaacct gcgcaccggg cgcaaggtct
      841 gggaatcttc cgtccctgaa cgcatgacgg gcggcccggt tctcgatcaa ggattgggcc
      901 tggtctacgt cctcacctgg tcggggcatc tgctggccta tgacgcgcat agcggagcgt
      961 tgcgctggaa aatcgcgctg ccgggcgggt cggaatcctc gcccgctctg gacgagcaaa
     1021 cgcacacgct ctatcttggc agcttcgacg gctcgctcta tgccatcgat gccaggaccg
     1081 ggcatgtgca atggcgactt gccaccggca gccccgtcac cgcctcgccg ctcgtcgtac
     1141 agggcgcgac ccaaagccgc gtgatagccg cgactcaagc cggcctatgc atcatggtcg
     1201 atgctcgctc cggtcggcgg ctcaacacgt ggaatcttgg agaactgcgc gccgcgcccg
     1261 ttatggcgca gggcatcctt tacctggcgt cgttaggcaa acagggcctg tttgccctcg
     1321 aattacaatg aatggagatt aatgaaagca agtgggtaga aagctttcat ttccacgcaa
     1381 agtactattg ctgctgttgc tgataccgct tggcgtgggt ctgacctatg catggcaata
     1441 cctgcatccc ccggcgccga aaccacgacc agcgtcgccc ccggtcctga cctataagcc
     1501 ggtcgtgggc gatgtgaacg acccggagga ggatgacccg gatattgcca tacagcaatc
     1561 gcttatcccc ggcttccagt tcaagcaggg gggctatgta ttgagcggca tagcgcgcga
     1621 cgcgaggacg ggacagccgg tagcagacgc tgttgtgtgg attggactgc ctgcgcgggc
     1681 ggggcaacct accagccccg ctttacatac ggtggccgac gcgggcggaa actttcagtt
     1741 caaccacctg gcgacggatg tctatacgct ggtcgccagt cgctattaca acatcggcga
     1801 taaccgctat tatgccgagc gtgtgttctc gcatgtgacc ttgcagggta atcgttccgg
     1861 cctggtattg tctctcacct cgatccccgc ccctggcagg cgatcaatcg gcacgggacg
     1921 gccacagaat ctgatcgtaa tcgatctgcg cgggttctac gccgattctc tgctcaatga
     1981 tccactgctg gtgaatgaga cgcccaacct gcgcgcgttt ttgcggtact ccacgaccct
     2041 gacatcgctc tggcagccct atgggtggag gccgccggat caatatgccc tgctcaccgg
     2101 cagctatccg cgctgggcaa cctacgacac gtggccgaat ccggttccct ggggcgtgcc
     2161 cgatggcatt gacacgcagt tctggtttac cggcgggcga agcgcgcatt tgttcgggca
     2221 ggaatccatc tttgacgtgg cgaaaggcta tgggatgcag acaggagtgg tcgccgggtc
     2281 agactttgtg ttgagcgacg ccacgacgcg caaccttgac ctgctccagc aggatagcgc
     2341 cttcgccccg cgaaactggt tagctcaaat gcaagacgct gtcctctccg gcgcgcagca
     2401 ggcgaacggc tttatgctct atggcgaact ggcgccgctc tctgctgatg atgcgaacgc
     2461 cagccccgac gcatcgggtg atgcctacca gcaggcgctg ctgcgagccg atcaaaccat
     2521 tggacaactg ctcgcctggc ttggtcaaca gggcctcttg cagcacaccc tcatcgcgct
     2581 caccaccagc caggcgcagg ccaatcacac cgacgccgac aactattacg gtatgggaat
     2641 caccggtcag ggaacgagca aacaaaccat gctcgcgctt tcctggccag gaggctgcat
     2701 cggacgcggc ggcacaagcg atacgaccgt gtattccagt tttgtcatcg cgcccatcct
     2761 gatgcgcgtc atgggcctgc ccgcgccagc cgaggccagg ttgtccgccc caggaggatg
     2821 tatatgagca tattacttcg taggggccga tttatcgcgc ccaccgccga tttatcggcc
     2881 tcgtcctggt tggtggcggc gatatcacag gccgataaat cggcggt
//
LOCUS       DASZAK010001262         8662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_174117, whole genome shotgun sequence.
ACCESSION   DASZAK010001262 DASZAK010000000
VERSION     DASZAK010001262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8662
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174117"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..828)
                     /locus_tag="VGT44_22170"
     CDS             complement(43..828)
                     /locus_tag="VGT44_22170"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEV2583583.1"
                     /translation="MTPTEQTAGKRPTTNGHAPATAQTPKSKRIVSANQRRAEIVIAT
                     LFLLTAAASIPAGFFLDPLFNASGYLAWVFPHQGAVALDAVLWSINNIGIVFIAVFAF
                     PVLRKLDETLAAGYLASRIIEGTIMMVGIMATLSLIPLSQEFLKAGAPQNSWFLTLGD
                     VLKQAKFLGLSEVSLPMLGLGGSLFTWMLFRYRLVPRVISVVGLIGYTLVFCYSIAGW
                     FGLIDVTPGASGVGVLVLPVATFEIILLPFWLFFKGFKTPEAP"
     gene            complement(1039..1860)
                     /locus_tag="VGT44_22175"
     CDS             complement(1039..1860)
                     /locus_tag="VGT44_22175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583584.1"
                     /translation="MATTERTSVIKQTVPLKGDKHVKTNRETSATRENNMKITPSKLI
                     RWSGVAAILAGLIFAAYQPIQPPEVLSSVTTSAWAIITPLKTAMCLLFLLGWTGLYAR
                     QVKKAGWLGLAGYLLLSLSWALQFAFVFATAFILPLLATTAPTFVDSLLRSASGTVSS
                     VNLGALPMLYTLVGIGYILGGLLFGIATLRAGILSRWAAGLLVVASALPLAFVLPSVA
                     ALIPPNIQHLAAMPLGIAVAWLGYSLLSERRAQASESSADRVSLQFSQTAAEYVR"
     gene            complement(1885..2142)
                     /locus_tag="VGT44_22180"
     CDS             complement(1885..2142)
                     /locus_tag="VGT44_22180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007913694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583585.1"
                     /translation="MSARHASIEDHDEPAFYEIRLKGHLDDRWADWFEGLTLTREDNG
                     ETLLTGPVVDQAALHSLLRKVRDLGMPLLSVTRVRRGQDDT"
     gene            complement(2356..5046)
                     /locus_tag="VGT44_22185"
     CDS             complement(2356..5046)
                     /locus_tag="VGT44_22185"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEV2583586.1"
                     /translation="MPTPILATKLYLPRLRPNVVSRPRLLERLNEGLHRKLILISAPA
                     GFGKTTLVSQWLAGGPRPAAWLSLDEGENDPSRFLAYLVAALQTIAAHIGEGVLGVLQ
                     PSQPPPSEAILTALLNEIITLPDPFVLVLDDYHLIDAKAVDSALTYLVEHLPPQMHLV
                     IATREDPQLPLARLRARSHLTELRAADLRFTASEAAAFLTQVMGLSLTAADIAALENH
                     TEGWIAGLQLAALSMQGQQDAAGFIRAFAGDHRYIVDYLVEEVLQRQPAPVRGFLLQT
                     SILDQLGGSLCDAVTGQEEGNARLEALERGNFFVVPLDDTRHWYRYHHLFAEVLSAHL
                     RAEQPDQVSILHRRASAWYEQHGSVVDAIRHALEAVDFARAAGLIELAVPAMRQSRQE
                     AMVLGWLKALPDELVRARPVLSLHYAGMLLLNGQLEGIEARLRDVEGWLDTTADRSGM
                     VVVDEEEFRRLPGGIALYRAAMAQARGDVSDTMHYARRVLDLAPEDDHLLRGSAAGIL
                     GLAFWASGDLEGACRMYAECIARVQQVGHISDTIGLSIALADIRIAQGRLREAMHTYE
                     RGLQLATEQGKPVLRGAADMYVGMSELHRERNDLHAATQLLAQSKELGDLLGLPQNRY
                     RWCVAMARIREANGDLDGALDLLNEAERLYMGDFFPNVRPIAALRTQVWVAQGRVSDA
                     LGWTREQGLSVSDDLSYLREFEHITLARVLLARYRSDHADRSLREAMGLLERLLQAAQ
                     AGERTGRAIEILIVQALAHQVQGDIPAALIPLHRALSLAEPEGYIRIFVDEGPPMALL
                     LEKAAKHGIAPNYVHQLLAATGRAPGRTPVTQVLIEPLSERELEVLRLLCTDLNGPEI
                     ARELVVSLNTMRTHTKNIFSKLGVDNRRAAVHRAEELHLF"
     gene            complement(5200..5433)
                     /locus_tag="VGT44_22190"
     CDS             complement(5200..5433)
                     /locus_tag="VGT44_22190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583587.1"
                     /translation="MTGYSCYIEQFPHGLPHREEAELRPCGHYACAPHTITYYGTGDD
                     DELVGDYCLVCYAKTFPQHCPDKLLLQAVMRDE"
     gene            5621..6607
                     /locus_tag="VGT44_22195"
     CDS             5621..6607
                     /locus_tag="VGT44_22195"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEV2583588.1"
                     /translation="MSTPISEMAKFSFGSKIFCADGENGILNGMSIDPASRRLVGIAI
                     KQGRFFGKVVYTAFEHVTGASSEGVKLDVKRGELATVAPFGVNLDDKSVVERVGSTHA
                     GVLKLVAVHPGSGELAYIVAHNLRPGHDVMLRAEYVTALAPERISVTIADDALNALPS
                     YRSDAELQQDVEAVLFDFTPMHIDLKGIKARVLDGVLYLDGNISSSLRSDIAQDQVYG
                     VEGLLEVKNNLLADDSLANDISQALAQDPRTRGLSIGVYPRLGDVRLSGAARTAQQKA
                     AAEEIARTVPGTRSVLNTLVIDPKADMLYVMSSAEGGEMEDKVPGKYVRHTQ"
     gene            6619..7977
                     /locus_tag="VGT44_22200"
     CDS             6619..7977
                     /locus_tag="VGT44_22200"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2583589.1"
                     /translation="MPTKPRDIATQALKELWQELLPDAGLPLIFASPLQRKWPVEHHP
                     RLLDQLVADGKGKKPAPLKAWRMLEAPSNAQGIAAVGEVSALLTPDMVRPAEAGERGA
                     IPASPYGWLHYTFLKGWEALSVYFYDSDSSESYVFNAIPESRQDEWLAFLKQLEDAHD
                     QIWRRGRRGVIEILGGPDEMAEAFRRASLDDLVLPPETFAHIAAQRNIFKKEVLERYA
                     ALHLPRLRKVLLVGPPGTGKTTLLKAEGAQHLKQGGQVLYVCASPSRNSSPWSQLSLA
                     LTLAADSRLPTLVLVEDLELFVSNAPEIQQILNTLDGASTPDNPGGTLLLATTSDPEK
                     IDLRIRDRTGRIDVIIEIGLVEDVEFATGLLSHFLGAAYREEVHTPVAPKLLKQPGSH
                     FREVCIAATLRALERESSEVLADDLLWAHEAIMTGKDVADEAARFIPSPARKRGSYFG
                     RT"
     gene            complement(8033..>8662)
                     /locus_tag="VGT44_22205"
     CDS             complement(8033..>8662)
                     /locus_tag="VGT44_22205"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEV2583590.1"
                     /translation="TTPYLLRPLEHGFDFVIHSATKYMGGHGDTTGGALITSKNAYLQ
                     QARGYATMLGAMLGPFESFLIARGLKTLAVRMDRHCYNALQVARFLQQHPAVARVHYP
                     GLSNHPHHELAARLLRHGHSGGLLSFELREQSREAAFRFMNRLKLCLPATTLGDVYSE
                     VSYPPMSSHRNLTPAERSAFGISEGCIRFSVGIELLEDIIRDLNQALEQ"
BASE COUNT         1751 a         2510 c         2637 g         1764 t
ORIGIN      
        1 ctcaggcttc agcagatcag catcgctacc tctgcgagga tgctagggag cttccggtgt
       61 cttgaagccc ttgaagaaca gccagaaggg gagcaggatg atctcgaacg ttgcgacggg
      121 aaggacaagg acgcccaccc ctgaggcacc aggtgtaacg tcgataagac cgaaccagcc
      181 ggcaatgcta tagcaaaaca ccagggtgta gccaatcaaa ccaactacag agatcacccg
      241 tggaacgagt cggtaccgga acaacatcca ggtaaagagc gatccaccca gcccgagcat
      301 cggtaaagaa acttctgata ggcccaggaa tttcgcctgc ttgaggacgt cgccaagagt
      361 cagaaaccac gagttctggg gcgcaccagc cttgaggaac tcctggctca acgggatcag
      421 cgacaaggtc gccatgatgc cgaccatcat gatggtgccc tcgatgatgc gggacgccag
      481 gtagccggcc gccagggttt catcgagttt tctcagcacg ggaaacgcaa acacggcgat
      541 gaagacgatc ccgatgttgt tgatcgacca cagcacggca tccaatgcga cggctccctg
      601 gtgcggaaac acccaggcca gatagccgga ggcgttgaaa agcggatcca ggaagaaacc
      661 cgcggggata ctggcggccg cggtcagcag gaacagggtc gcaattacga tttctgcccg
      721 gcgctggttg gcactgacga tgcgctttga tttcggagtc tgcgcggtcg cgggggcatg
      781 gccgttggtc gtcggtctct ttcctgcagt ttgctcggtg ggagtcatgc gtgtgtctcc
      841 tttcatagtt gcggtctgct tgtcgtcgcg tctgcgctgc ccctcgcgtt cgtgctgccc
      901 tccgttgctg cgctgagccc gcccaatatc cagcatttag cagcgatgcc gctggggata
      961 gcggtggcct ggccgggttg aggcgggttc ccggtgagac aagcggccgt gcggccgctt
     1021 gtccattccc tgccgtttct agcgaacgta ctcggcggcg gtttggctga actggaggct
     1081 taccctgtca gctgaagatt ccgaagcttg cgctcgccgt tctgacaaga gcgaatagcc
     1141 cagccaggcc accgctatcc ccagcggcat cgctgctaaa tgctggatat tgggcgggat
     1201 cagcgcagca acggagggca gcacgaacgc gaggggcagc gcagacgcga cgacaagcag
     1261 accggcggcc cagcgcgaca ggatgccggc gcgcagcgtc gcgataccaa agaggaggcc
     1321 gccgaggata tacccgatcc cgacgagcgt atagagcatt gggagggctc cgaggttcac
     1381 gctgctcacg gtcccagagg cgctcctcag gaggctatcc acaaatgtgg gtgccgtggt
     1441 cgccaacagc ggcaggataa aggcggtggc gaagacaaag gcaaactgga gcgcccaact
     1501 gaggctcaac aggagatagc ctgccagacc aagccacccg gcctttttca cttgcctggc
     1561 gtagagcccg gtccagccca gcaggaagag gaggcacatg gccgtcttca agggagtgat
     1621 gatggcccag gcgctggtcg tgacggacga aagcacttca ggtggctgga tcggctgata
     1681 tgctgcgaaa atgagccctg ccaggatcgc tgctacaccg ctccagcgga tgagcttcga
     1741 aggtgtgatc ttcatgttgt tctcacgcgt ggctgaggtc tcgcgattgg tcttcacgtg
     1801 tttgtctcct ttcaagggta cagtctgctt gattacgctc gttcgttcgg tggtagccat
     1861 cgttttttct ccttttttgg catctcacgt atcatcttgg ccgcgtctga cgcgggtgac
     1921 cgagagtaag ggcatgccta aatcacgcac ttttctgagc aagctatgca aggcggcctg
     1981 atcgaccacc gggccggtta acagcgtctc gccgttgtct tctcgtgtca gggtcaggcc
     2041 ctcaaaccaa tcggcccacc tgtcatcaag gtgccccttg aggcgaattt cataaaacgc
     2101 gggctcgtca tgatcctcaa ttgatgcgtg ccttgcactc atgctgctct tcgtgccctt
     2161 tcctctctct ggatgaagct ctacgtcgtt gtaatcgttc tttgcttctg atcagaagat
     2221 acacgagaat gtggtgagta ggcatcacca aatgtggtga ttgatgtggt gatttgttga
     2281 tcggaggcta tgagggagaa aaaagccccg gtaggtggtt cgacacacct gccggggttg
     2341 gtgtttgcgg cttcgttaga acaaatggag ttcctcagcc cggtggacgg ctgcccgacg
     2401 gttgtctacc ccgagcttgc tgaaaatgtt tttggtgtgt gtacgcatgg tgttgaggga
     2461 caccaccagt tcgcgagcaa tttccgggcc gttcaagtcg gtacaaagca gccggagcac
     2521 ttccagttca cgttccgaga gtggctcgat caacacctgg gtgacgggcg ttctgcctgg
     2581 agccctgccc gtggctgcca gaagctgatg aacatagttc ggggcgatcc cgtgtttggc
     2641 ggccttttcc agcaggagcg ccataggtgg gccttcgtcc acaaagatgc ggatgtagcc
     2701 ctctggctca gccagcgaga gtgcccgatg cagcgggata agtgcagcag gaatatcgcc
     2761 ttgtacctgg tgagcgagtg cctgcacgat caggatttcg atcgctcttc ccgtccgctc
     2821 ccctgcttgc gctgcttgta ggaggcgctc gagcagtccc attgcctcac gaagggagcg
     2881 gtctgcatga tcgctcctat accgggccag gagcaccctg gccagggtaa tgtgctcgaa
     2941 ctcccgcagg tagctcaagt cgtccgaaac ggagaggcct tgctcgcgcg tccagccaag
     3001 ggcgtcactc actctcccct gcgcgaccca tacctgtgtc cttagcgcgg ctataggacg
     3061 cacattgggg aagaagtcac ccatatacag gcgctctgcc tcgttgagga ggtcgagcgc
     3121 gccatccaga tctccgttgg cctcccgtat gcgagccatc gctacgcacc agcgataccg
     3181 gttttgcggc aaccccaaga gatcccccag ctccttactt tgcgccagga gttgcgtcgc
     3241 ggcatgcagg tcgttccgct cgcggtggag ctcgctcata cccacgtaca tatctgccgc
     3301 tccccgcagc accggcttgc cttgttccgt tgcaagctgc aatccccgct cataggtatg
     3361 catcgcctcg cgcagacgac cctgggcaat ccgtatatca gccagggcga tagacaaacc
     3421 gatggtgtca gagatgtgac cgacctgctg cacacgcgct atgcactctg catacatgcg
     3481 gcatgctccc tccagatccc cacttgccca gaatgcgagt cccagaatcc ccgctgccga
     3541 tccacgcagg agatgatcgt cctcgggggc gagatcaagc acgcgccggg cgtagtgcat
     3601 ggtgtcggac acatcgcctc gagcctgggc cattgcggca cggtacagag cgatcccccc
     3661 cgggagacgg cgaaactcct cttcgtccac aacgaccatc ccactcctgt cggctgtcgt
     3721 gtccagccac ccttctacgt cccgcagtcg ggcctcgatg ccctcgagct ggccgttaag
     3781 cagtaacatc ccggcatagt gaaggctgag tacgggcctg gcgcgcacca gctcgtcggg
     3841 gagcgccttg agccagccaa gcaccatggc ctcttgtcta ctctggcgca tggctggcac
     3901 agccagctcg atcaggcccg ccgctcgcgc gaaatcaacg gcttccagcg cgtggcggat
     3961 cgcatcgacc actgagccat gctgctcgta ccacgcgctc gcgcgccggt gcagaatgga
     4021 tacctgatcg ggctgctccg ccctcaaatg agcagagagc acttcggcaa agaggtggtg
     4081 atagcgatac cagtggcgcg tgtcatcgag cggaacgaca aagaagttgc ctcgctccag
     4141 agcctccaac cgcgcgttgc cttcctcctg gccggtgacg gcatcgcaga gcgaaccacc
     4201 caactggtcg agaatggagg tctgaagtaa gaagccgcgc acaggtgcgg gctgacgctg
     4261 caggacctct tcgaccagat agtccacgat gtagcggtgg tctcccgcga aggcccggat
     4321 gaacccggca gcgtcctgct ggccctgcat cgaaagcgcg gctaattgca ggccggcaat
     4381 ccagccttcg gtgtgatttt ctagcgcggc gatgtctgcc gccgtgaggc tgaggcccat
     4441 cacctgggtg agaaatgcgg cggcttcaga ggcggtaaac cgcaagtcgg cggcgcgcag
     4501 ttcggtcaag tggctccggg cgcgtaagcg ggccaggggt aactgtggat cttcgcgggt
     4561 ggcaatcacc aggtgcatct gaggcggcag gtgctcgacg agataggtga gggccgagtc
     4621 taccgctttg gcatcaatca agtggtagtc gtccaggacg aggacgaacg ggtccgggag
     4681 cgtgatgatc tcattgagca gggctgtcag gatcgcttcg gatggcggtg gctgacttgg
     4741 ttggagcaca cccaacaccc cttccccaat atgtgccgca atcgtctgta aagcagcgac
     4801 caggtaagcc agaaagcgtg aggggtcgtt ctccccttcg tccagcgaca gccaggcggc
     4861 gggtcgaggg cctccggcaa gccactggct gaccagcgta gttttcccaa agccggcagg
     4921 agcagaaatg aggatcagtt tgcggtgcag accctcgttg agccgctcga gcaggcgggg
     4981 acggctgacc acgttcggtc gaagccgcgg gagatacagt ttcgtggcga gaatgggcgt
     5041 cggcataccg gcttattttc cttctaacgg ttcacacgag cacgagtaaa agagtactat
     5101 cctacggtgc gttgttccat ctactcaact ccatctatgc tgttcctgtt ttgctttggc
     5161 tatccattgg agagcctgcc agggtcagct atctcttgct tattcatccc gcattaccgc
     5221 ctgcaatagc agcttatcag ggcaatgctg gggaaacgtt ttcgcgtagc acaccaggca
     5281 ataatctcct accagttcgt cgtcgtcgcc ggtgccatag taggtgatgg tgtgcggcgc
     5341 gcaggcgtaa tgcccgcagg ggcgcagttc ggcctcttcg cggtgcggca ggccatgcgg
     5401 aaattgctcg atatagcagg aatatccggt catagtaaaa tcctcgcttg tcatgtatcc
     5461 attgaacgtg ctacacttct agtaaggaca gggcagcaca gcatcaacag cagcctgtgt
     5521 ctctttatta tattacagtg cgaggttggt tgtatgagaa cattgatggc cagacctcca
     5581 acgcactgcg tgctagcgct gcgcgctctg gaggtttgat atgagtactc ctatttcaga
     5641 aatggccaaa ttcagctttg gaagcaagat attttgtgct gatggtgaaa acggtatctt
     5701 gaacggcatg agcattgatc ctgcatcgcg ccgcctggtt ggtattgcca tcaagcaggg
     5761 gcggttcttt ggcaaggtgg tctacaccgc gttcgagcat gtcaccggcg cttcgagtga
     5821 aggtgtaaag ctcgatgtca aacgtggtga gttggccaca gttgccccat tcggcgtcaa
     5881 tctcgatgac aaaagtgtcg tagagcgcgt cgggtccaca catgcgggcg tcctcaagtt
     5941 ggtagctgtt catccaggga gcggcgagct ggcctatatt gtggcgcaca atctgcgccc
     6001 cggccatgat gtgatgctgc gcgccgagta tgtcaccgcc cttgcgccgg agcgcatcag
     6061 cgtcaccatc gcggatgacg cgctcaatgc gctcccgtcc tatcgctcag atgccgaatt
     6121 gcagcaggat gtggaggccg tgttatttga cttcacgccc atgcatatcg acctcaaagg
     6181 catcaaggcg cgtgtgctgg atggcgtcct ctacctggat ggcaatattt ccagttcgct
     6241 gcgttccgat atcgcgcagg atcaggtcta tggggtagag ggcctgctgg aggtcaagaa
     6301 taacctgctg gccgatgatt cgctggcaaa cgatatctcg caggcgctgg cccaggaccc
     6361 gcgcactcgt ggactgtcta tcggcgtcta cccgcgcctg ggcgatgtgc gcttgagcgg
     6421 cgcggcgcgc acagcacagc aaaaggcagc cgcggaagag attgctcgca ccgttccagg
     6481 gacgcgctcg gttctcaata cgctcgtgat tgaccccaag gccgatatgc tgtatgtcat
     6541 gtcatcggcg gaagggggcg agatggagga taaggtgccg gggaaatacg tccggcatac
     6601 gcaataaggg gtacagctat gcccacgaaa ccccgtgaca tcgcgacgca agccctgaaa
     6661 gaattgtggc aagagctact gccggatgct ggtctgccgc tgatcttcgc ctcgcccttg
     6721 cagcgtaagt ggccggtcga acatcacccg cgattactcg accagctcgt agcagacggc
     6781 aaaggcaaga agccagcgcc gctaaaggcc tggcgcatgc ttgaagcgcc ttccaatgcg
     6841 cagggcatcg ctgcggtcgg cgaggtcagc gcgctgctca cgccggatat ggtgcgcccg
     6901 gctgaagcag gcgagcgcgg cgcgatcccg gcgtctccct atggctggct acactacacc
     6961 ttcctgaagg gctgggaggc gctttccgtc tatttttacg actccgacag tagtgaatcg
     7021 tatgtcttca atgccatacc ggagagtcgc caggatgaat ggctggcgtt cttgaagcag
     7081 ttggaggacg cgcacgatca aatctggcgg cgcggcaggc gcggcgttat cgagattctc
     7141 ggcggcccag atgaaatggc agaagccttt cgacgcgctt cgcttgatga tctcgttctg
     7201 ccgcctgaaa ccttcgcgca tatcgccgcc cagcgcaata ttttcaagaa agaagtgctg
     7261 gagcgctatg ccgcgctgca tcttcctcgc ctgcgcaaag tattgctcgt cgggccgccc
     7321 ggcaccggca aaacgacgct gttgaaagcc gagggggcgc aacatctcaa acagggcggg
     7381 caagtattgt atgtctgtgc ctcgccatcg cgcaactcca gcccctggag tcagctctcc
     7441 ctcgccttga cgctagccgc ggatagtcgt cttccaaccc tggtgctggt cgaggattta
     7501 gaactgtttg tttcaaatgc gccggagata cagcaaatct taaataccct ggatggcgcg
     7561 tcgacgccgg ataatccggg aggaaccttg cttctggcaa ccacgagcga cccagaaaaa
     7621 atcgatctgc gcatccgcga tcgcaccggg cgcatcgacg ttatcatcga aattggcctg
     7681 gtagaagatg tagagttcgc gacgggtctg ctgtctcatt tcctcggcgc tgcctaccgc
     7741 gaggaagtac acacgcccgt tgcgccaaag ctgctcaagc agcccggcag ccatttccgt
     7801 gaagtctgta ttgccgcgac cctgcgcgcc ctggaacggg aaagtagcga ggtgcttgcc
     7861 gacgatctcc tctgggcgca cgaggcgatc atgacgggca aggatgtcgc ggatgaggcc
     7921 gcgcgcttta ttccttcccc ggcgcgcaaa cgcggaagtt atttcggtcg cacgtagcag
     7981 cccgtcaaag ttcgatgggc gtgtaggggc caatgacaac tttttaccga tcttactgtt
     8041 ccaacgcctg gttcaggtct cgtataatgt cctccaacaa ctcgatgccc actgagaagc
     8101 ggatgcagcc ctcactgatg ccgaacgcgg agcgttccgc cggggtcagg ttgcggtgcg
     8161 aggacatggg cgggtagctt acctctgaat acacgtcgcc gagggtcgta gcgggcaggc
     8221 agagcttcag ccggttcatg aagcggaaag ccgcctcgcg tgattgctcg cgcagctcga
     8281 acgacagcag accgccggaa tgcccgtgcc ggagcaggcg cgcggccagt tcgtgatggg
     8341 gatggttgct caatcctgga taatgcacgc gcgcgaccgc aggatgctgc tgtaagaagc
     8401 gcgcaacctg caatgcgttg tagcagtgac gatccatgcg caccgccagt gtttttagcc
     8461 cgcgcgcgat cagaaacgat tcaaatgggc cgagcattgc gccgagcatg gtggcatagc
     8521 cgcgcgcctg ctggagatag gcgttcttcg aggtgatgag cgcgccgccg gtcgtgtctc
     8581 catgcccacc catatacttc gtcgcgctgt gtatcacaaa atcgaagccg tgttcgagcg
     8641 gtcgaagaag ataaggcgtc gt
//
LOCUS       DASZAK010001263         1641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_66952, whole genome shotgun sequence.
ACCESSION   DASZAK010001263 DASZAK010000000
VERSION     DASZAK010001263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66952"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VGT44_22210"
     CDS             complement(<1..242)
                     /locus_tag="VGT44_22210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HEV2583591.1"
                     /translation="MAKVKITRGKYEGLNACSDKRGVIAALAVDHRGNLLQEIARSRG
                     ESGTASAADMLAFKTAVVKTLTPHASAILLDPEYGLG"
     gene            complement(258..1277)
                     /gene="iolG"
                     /locus_tag="VGT44_22215"
     CDS             complement(258..1277)
                     /gene="iolG"
                     /locus_tag="VGT44_22215"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HEV2583592.1"
                     /translation="MLVNSLKMGLIGAGRIGLLHAEHLTSRVPEADLLMVADTFEQAA
                     RECAERYSIPFFETDYHAILNHPDIQAVVICSSTNTHARIIEEAALAGKHIFCEKPIA
                     LDLASIDRALDVVERAGVKLQIGFNRRFDANYRRVRQVVEEGVIGQPHLLHIVSRDPD
                     PPSIEYIRVSGGIFLDMTIHDFDMARFLIGSEVEEIFAMGGVMVNPAIGAAGDVDTAV
                     VLLHFSNGVIGTIDNSRQAAYGYDQRVELLGSGGAISTGNDYPNTAVISDSRSVRRDL
                     PLHFITERYTESFVAEMVAFVDAVLRDEPVAVTGHDGRVPVVMALAAGKSLAERRPVR
                     LSEIE"
     gene            complement(1300..>1641)
                     /locus_tag="VGT44_22220"
     CDS             complement(1300..>1641)
                     /locus_tag="VGT44_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HEV2583593.1"
                     /translation="GHYLYGTGSNDPTRVEDGLHRFGERIWHVHFKDCQPRVAEEART
                     SGWDYFTALRHGVFCELGQGRVPFGAVVAWLRARNYDGWIVVEQDILPGMGSPKASAE
                     RNRAFLSSIGL"
BASE COUNT          382 a          498 c          428 g          333 t
ORIGIN      
        1 ccaagaccgt attctggatc taagagaatt gcgctggcat ggggagtcag ggttttgacg
       61 accgctgttt tgaacgcaag catatctgcc gccgaagcgg ttccgctttc accgcgtgac
      121 cgggcaatct cctgcaggag attgccacgg tgatctaccg ccagcgcggc gatgacacca
      181 cgcttgtcgg aacaggcatt cagaccttca tatttgcccc tggttatctt gacctttgcc
      241 atagaattcc ccgttcatta ctcgatttcg ctgagccgga ctggccggcg ttccgcgagg
      301 gacttaccgg cagccagcgc catgaccacc gggacacgcc cgtcatgccc ggtgacagca
      361 acaggctcgt cacggagcac agcatcgacg aaagccacca tctcagcgac aaagctctcg
      421 gtatagcgct ccgtgatgaa gtggaggggc aagtcgcgcc ggacactgcg actatcgctg
      481 atgacggctg tgttcggata gtcgtttccc gtggagatag cgccgccact acccagtaat
      541 tccacgcgct ggtcatagcc ataggcggct tgacggctgt tatcgatcgt gccaatcacc
      601 ccattgctaa aatgcaacag gacaaccgcg gtatccacat cgcctgccgc tccgatggca
      661 gggttaacca tgacgccccc catggcgaag atctcctcca cctcactccc gatcaaaaaa
      721 cgggccatgt cgaagtcatg gatcgtcata tccaggaaga taccaccaga cacccggata
      781 tattcaattg atggcggatc ggggtcacgg ctgacgatat gcagcaggtg aggttgaccg
      841 atcactccct cttctacaac ctggcgcacg cggcgataat ttgcgtcaaa acgccgattg
      901 aagccgatct gaagcttcac accggcccgt tccacgacat ccagcgcccg gtcaatcgac
      961 gccaggtcga gagcgatcgg tttctcgcag aaaatgtgct tgcctgccag tgccgcctct
     1021 tcgatgatcc gggcatgggt gttggtagaa gaacagatca ctaccgcttg aatatctgga
     1081 tgattcaaaa ttgcatgata atctgtctcg aagaaaggaa tagagtagcg ctccgcacac
     1141 tccctggccg cctgctcgaa tgtatcagcc accatgagca ggtcggcttc agggacacgt
     1201 gaggtaaggt gctcggcatg caagaggccg atacgtccag cgccgatcag gcccatcttc
     1261 aatgaattta caagcacagg catctccttt ctttgaccgc tacaacccaa tggagctaag
     1321 aaaagcccgg ttgcgctcag cacttgcttt gggacttccc atgccaggca gaatatcctg
     1381 ctccacaaca atccaaccat catagttgcg cgcgcgtagc cacgcgacaa ctgccccaaa
     1441 tggcacgcga ccctgaccca gttcacaaaa caccccgtga cgaagggctg tgaaatagtc
     1501 ccagccactg gtacgagctt cttctgcaac ccgcggctgg caatctttga aatggacatg
     1561 ccagatgcgc tcgccgaaac ggtgtagccc atcctcgaca cgcgtgggat cattgctgcc
     1621 ggtcccgtag aggtaatggc c
//
LOCUS       DASZAK010001264         3320 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_530457, whole genome shotgun sequence.
ACCESSION   DASZAK010001264 DASZAK010000000
VERSION     DASZAK010001264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530457"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VGT44_22225"
     CDS             <1..673
                     /locus_tag="VGT44_22225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583594.1"
                     /translation="AVVNRVDAGLFSFNSKGACPNCQGLGVTYVDLAFLEDVKQPCEV
                     CGGKRFKDEVLAYTFNGKSIADVLSMTINQALSFFTMKEVRPKLQAMSDVGLDYLTLG
                     QPLSTLSGGECQRMKLASELHKQGSIYVMDEPTTGLHLSDIGRLLTIVNRLVDGGNTV
                     IVIEHHLDVIRNADWIIDLGPEGGNKGGTVIFEGTPRELLGARHSLTSQYLSQTGWPS
                     GGQAH"
     gene            complement(773..2230)
                     /locus_tag="VGT44_22230"
     CDS             complement(773..2230)
                     /locus_tag="VGT44_22230"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV2583595.1"
                     /translation="MMSRAPYSLEEVLKPRAVAILGASRSHHKWGHVAARQLIEGGFP
                     GELYLVNPSVPEVLDRATYPSLRDVPSQVDLAIVATAFQHVPQAIDDCIAHGVKGIVI
                     ITAGFSETGPHGRALEEELVARCREHGIRVIGSNCMGIYVRNAQMNALGMAFPLPDGP
                     IGLVSQSGNLGMYFYAQAHLDGLGFTTFLSVGNAADVSFPECIAYLAGDPHTCVIAGY
                     VEAIQEQTLREVTRDMRAQGRYKPIVILLSGATEVGVRASLAHTGTISTIRPDHDTSL
                     LGSGVVRVVRSDELFPVAQALATQPPTPLGRNRIAIIGDGGGSVVATGDAAVRAGLAV
                     PILCDDTQARLRQLMPARATSTNPVDVAGAADEDPLAFAWLSEVCLKDPTIDGVIITG
                     LFGGYRWLLSEAFGPREEAAARELGRLVRHYHKPVLVQTIYARHDIPALRILRETGIP
                     YYESIEITCRAMAALSEVGLFYKQNSADGDSHCVS"
     gene            complement(2227..3195)
                     /locus_tag="VGT44_22235"
     CDS             complement(2227..3195)
                     /locus_tag="VGT44_22235"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEV2583596.1"
                     /translation="MAFVHYLNKPELRSPVVIAAFSGWNDAADAATTAVKVLVDRWEP
                     TKFAEIDAEEFFDFTETRPTVAANKGRVRSISWPSNTFMAHTRPSMERDVILFLGSEP
                     QLKWKTFTSTFLEVCKGFEVSEVLFLGAFLANIPHSFDVPISGLSSTEEMQERLNEID
                     VQGTRYEGPTGIVGVLQEATTSANIPVSSLWAAAPHYLVATPNIKVTAALLTHANAFL
                     SFDLDLSDIQADATNFEEQITRLVARDPDASAYVRKLEEQIEQAVDDDDDDDDEETAE
                     SPSINPDRPTGTGPLPSADTLIRRVEELLRKERENNQHNGDSDEEQ"
BASE COUNT          665 a          934 c         1016 g          705 t
ORIGIN      
        1 tgcggtggtg aatcgcgtcg atgctggcct gtttagcttt aactctaagg gtgcctgtcc
       61 gaactgtcag gggcttggcg ttacctatgt cgacctggca tttcttgagg acgtcaagca
      121 accatgtgaa gtgtgtggcg gcaagcgttt taaggacgag gtgctggcct ataccttcaa
      181 cggcaaatct attgccgatg tcctgagcat gaccatcaac caggcactct cgttttttac
      241 gatgaaagaa gtgcggccaa agctccaggc gatgagcgat gtgggcctgg attacctgac
      301 gctgggccag cccctgagca cgctctctgg cggcgaatgc caacgcatga agttggccag
      361 cgaactccac aagcaaggca gtatctatgt catggatgag ccaacgaccg ggctgcacct
      421 gtcggatatt ggtcgcctgc tcaccatcgt gaaccggctg gtcgatggcg gcaacaccgt
      481 gattgtgatt gagcaccatc tcgatgtgat tcgcaacgcc gactggatca ttgatctggg
      541 tccagaaggc ggcaataagg gcggcacggt tatcttcgag gggacaccgc gagaactact
      601 cggcgccagg cattcgctga cgagccagta tttgtcacag accgggtggc cgagcggcgg
      661 tcaagcgcat tgactccaac ttcttcacct gtggagatgg gcgttgtccc ttccatggca
      721 tagatcggcg tacctcattt gggctgagga gtgccgctgc ccccgcatcc attcaagata
      781 cgcagtggga atcaccatcg gcgctgttct gcttgtaaaa caagccaacc tcgctcaatg
      841 cagccatcgc gcggcaggtg atctcgatgg attcgtagta gggaatgcct gtttcgcgca
      901 ggatgcgcag ggcggggatg tcgtggcggg cgtagatggt ctgcaccagc accggcttgt
      961 ggtagtggcg caccaacctg ccgagttcgc gcgcggcggc ctcttcgcgt gggccgaatg
     1021 cctcggagag cagccagcga tagccgccga acagcccggt gatgatgacg ccgtcgatgg
     1081 tggggtcttt caggcagact tcggacagcc aggcgaaggc cagggggtct tcatcggcgg
     1141 cgcccgccac gtctaccggg ttggtcgagg tggcgcgcgc gggcatgagc tggcgtagcc
     1201 tggcctgggt atcgtcgcag aggatcggga cggccagccc tgcgcgcact gccgcgtcac
     1261 cggtggcaac cacgctgccg ccgccgtcgc cgatgatggc gatgcgattt cttccaagtg
     1321 gcgttggcgg ctgcgtggcg agcgcctggg cgacggggaa gagttcgtcg gagcgcacga
     1381 cgcgcacgac gccgctgccg agcaggctgg tatcgtgatc gggtcggatt gttgaaatcg
     1441 tgccggtatg cgccagcgag gcgcgcactc ctacctcggt tgcgccactc agcaggatca
     1501 cgatgggctt atagcgcccc tgcgcccgca tgtcgcgtgt gacctcgcgc agcgtctgtt
     1561 cttgaatagc ctccacgtac ccggcgatga cacacgtatg cgggtcgccc gcgagataag
     1621 caatacattc ggggaagctc acgtcggcgg cgttgcctac gctcaaaaag gtggtgaagc
     1681 cgaggccatc caggtgcgcc tgggcgtaaa agtacattcc caggttgccg ctctgcgaga
     1741 ccagcccgat aggcccgtct ggcaggggaa aggccatgcc cagcgcgttc atctgcgcgt
     1801 ttctgacgta gatgcccatg caattgctgc caatcacgcg aatgccatgt tcgcggcagc
     1861 gcgccaccag ttcctcttca agggcgcggc catgcggccc ggtttcgctg aagcccgcgg
     1921 tgataatgac gatgcccttc acgccatgcg cgatgcaatc atcgatggcc tgggggacgt
     1981 gttggaaggc tgtggcgacg atggcaagat caacctgaga cggcacatcg cgcagcgacg
     2041 ggtaggtagc gcggtccaat acttctggca cggatggatt gacgagatac aattcgccgg
     2101 gaaatcctcc ctcgatcaac tgcctggccg ccacgtggcc ccatttatga tgcgagcgag
     2161 aagcgcccag aatggctaca gcgcgaggct tgaggacttc ctccagggaa tagggagcgc
     2221 ggctcatcat tgttcctcgt ctgagtcacc gttgtgctgg ttattttcgc gttccttgcg
     2281 cagcaactct tctacccgcc gaatcaacgt atcggcgctg ggcagcggcc cggtacctgt
     2341 cgggcgatcg gggttgatcg atggcgactc agccgtttcc tcgtcgtcat cgtcatcatc
     2401 gtcatcgaca gcctgttcaa tctgctcttc gagctttcgg acgtacgcac tggcgtctgg
     2461 atcgcgcgcg acaagacgtg taatttgctc ctcaaaattg gtggcatcgg cctggatatc
     2521 gcttagatcg agatcgaacg acaaaaaagc gttcgcatgg gtgagcagcg ccgctgtgac
     2581 cttgatattc ggcgtggcga ccaggtaatg cggagcggct gcccacaggc tggaaacggg
     2641 gatgtttgca ctggttgtgg cctcttgcaa gacgccaacg atgccggttg gcccctcgta
     2701 gcgcgtcccc tgcacatcga tttcgttcaa gcgttcctgc atctcctcgg ttgaagacag
     2761 gccggaaatc ggcacatcga agctgtgggg gatatttgcc aggaacgcac ccagaaataa
     2821 tacttccgag acttcgaagc ctttgcacac ttccaggaag gtagacgtaa acgtcttcca
     2881 tttcaattgt ggctcggagc cgaggaaaag aataacatcg cgttccattg aaggacgggt
     2941 atgcgccatg aaggtattgc tcggccagga aatagagcgc acacgccctt tatttgctgc
     3001 aacggttggc cgtgtttcgg taaagtcgaa gaactcttcc gcgtcgatct cagcaaactt
     3061 ggttggttcc cagcgatcca ccagcacctt gacggcggtg gtcgccgcgt cggcggcatc
     3121 gttccatccc gaaaatgctg cgataaccac cggactgcgt aactcaggtt tattgagata
     3181 gtgtacgaaa gccatctttc ctcccctata gatgcatgtg acattgcgaa atcgggttgt
     3241 acctattgta tcatgcatga aaagagcttg ttaaagacga atttcctata gccatatgat
     3301 attgaccagt cccatgatac
//
LOCUS       DASZAK010001265         3336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_244702, whole genome shotgun sequence.
ACCESSION   DASZAK010001265 DASZAK010000000
VERSION     DASZAK010001265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3336
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244702"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1787)
                     /locus_tag="VGT44_22240"
     CDS             complement(156..1787)
                     /locus_tag="VGT44_22240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV2583597.1"
                     /translation="MLLVNFSKVSKDFGGNQVFRDIDLDIMEGERIGLVGENGGGKST
                     LFKLLAGLDEPTSGTIAPRRNLTIGYLPQEVDHGLGHKTVFEAVSEATQELAGLPELL
                     SNLEERMADPAVANNPRELARVLEEYGRAQERFESSGGYEGSHNVEAVLHGLGFGPEW
                     YDMEVGALSGGEKKLVYLARILLKTPDLLLLDEPDNHLDLEAKAWLEEYIRSYPGTVV
                     IISHDRRLLDRAVKKVFELEDGRISTYAGNYSYYFEERQRRLQQQFELYTRQQDEIKR
                     LEGIWRQYQGWSKLNDKFATKAEDTLKRIERIKRDGVSKPVMLRDRIKVNLDADRSGK
                     KVLEIKGLSKAYNSHTLFRPFDFTMLYGERVGIVGANGAGKTTLLKTMLELLPADSGT
                     VKIGASVVPGYYAQEQETLPLDSTPLDFIRRLKPMNEPQAISFLSGLLFSYDDIRTPI
                     RRLSGGEKSRLQLARLTLSDANFLLLDEPTNNLDLASVEVLEVALQAFEGTILTVSHD
                     RYFLDKIVTKIVAIGDDGYVRVYPGNFSYYQAHRR"
     gene            2326..2631
                     /locus_tag="VGT44_22245"
     CDS             2326..2631
                     /locus_tag="VGT44_22245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583598.1"
                     /translation="MNLLNISRQRAAGLMTILLLMLLLAACGTPANAASSGSSQGTSA
                     QQTQQRQPTSSNATTSSAKSGMRSTDQQVQSLLRQLDGAQGDVNNADAGATQDSGQP"
     gene            2708..>3336
                     /locus_tag="VGT44_22250"
     CDS             2708..>3336
                     /locus_tag="VGT44_22250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583599.1"
                     /translation="MKLFVMFRKLTWRAAGVAVLALFALGALLPSTAFAATASSTPPC
                     ASTDTQCFIKLGDQFIAARQTALTTLSGKVSGRLNQQLISSDQAAVLQADISTNQQGL
                     SALETKLNGETNALAAKQDVVNVFFQFRIYAVVLPRDYRRLYLDVAITVDAKLRGFGP
                     KVQEAIQNAPASEQAQLKTLFSDYQNQLSTAEGQFDSAAADIVTLMPEN"
BASE COUNT          700 a          923 c          948 g          765 t
ORIGIN      
        1 gttacttccc gaccctcctg acgctccatt gctagaacca gaattccctg tatgataatt
       61 gctcaaggca agatgcctcc aatacttact actgatacca cttcattaag tataccacac
      121 tacctgacct ttttatagtc cattgtgata tgtccctacc gacggtgtgc ctgataatac
      181 gaaaagttgc cggggtagac gcgcacatac ccatcatcgc cgattgccac gatttttgtg
      241 acgatcttgt cgaggaagta gcggtcgtgc gagacggtca ggatggtccc ctcgaacgct
      301 tgcagggcga cctccagcac ttcgaccgag gcgagatcga ggttgttggt cggctcgtcg
      361 agcagcagga agttggcatc gctgagtgtc aggcgggcca gttgcaggcg gctcttctcg
      421 ccaccgctca ggcggcgaat gggtgtgcga atgtcgtcat acgagaagag caggccgctc
      481 aagaaagaga tagcctgcgg ctcgttcatg ggcttgagtc ggcgaatgaa gtcgagcggc
      541 gtgctgtcga ggggcagcgt ctcttgctcc tgggcgtagt agccggggac gacgctggct
      601 ccgatcttga ccgtgccgct atcggcgggg agcagttcca gcatggtttt gagcagcgtg
      661 gttttgcccg cgccgttggc tcccacaata ccaacgcgct cgccgtagag catggtgaag
      721 tcgaaaggcc ggaatagcgt atggctattg taggctttgc tcaacccttt gatctccagc
      781 acctttttgc cgctgcggtc ggcgtccagg ttgaccttga tgcggtcgcg cagcatgacg
      841 ggcttgctca ccccgtcgcg tttgatgcgc tcaatgcgtt tgagcgtatc ttcggctttg
      901 gtggcgaact tatcattgag ctttgaccag ccctggtatt gacgccagat gccttccagg
      961 cgtttgattt cgtcctgctg gcgtgtatat agctcaaact gctgctggag acgccgctgc
     1021 cgttcctcaa agtagtagct gtagttgccc gcgtaggtcg agatgcgccc atcctctagc
     1081 tcgaaaacct tcttgacggc gcggtcgagc aggcggcggt cgtgggagat aatcacgacc
     1141 gtgccgggat agctgcggat atattcttcc aaccacgctt tcgcctcgag atcgagatgg
     1201 ttatccggct cgtcgagcag cagcaggtcg ggggttttga gcaggatacg cgccaggtag
     1261 accagcttct tctcgccgcc gctcagcgca ccaacctcca tatcgtacca ctccggcccg
     1321 aaccccaggc catgcagcac cgcttcgacg ttgtgggagc cttcgtaacc gcccgatgac
     1381 tcgaagcgtt cctgcgctcg cccgtattcc tccagcacac gcgcgagttc gcgcgggttg
     1441 ttggcaacgg cgggatcggc catgcgctcc tctaaattac tcagcaattc cggcagtccc
     1501 gccagttcct gcgttgcctc cgagacggct tcaaagaccg tcttatgtcc cagtccgtga
     1561 tcgacttcct gtgggaggta gccgatcgtc aggttgcggc gcggcgcgat ggtgccgctc
     1621 gtcggctcat ccaatccagc cagcagcttg aagagcgtcg atttgccgcc tccgttttcg
     1681 ccgaccagcc cgatgcgctc gccttccatg atgtccaggt caatgtcgcg gaatacctgg
     1741 tttccgccaa agtctttgga gacttttgag aagttcacga gtagcatgtg tgcatctcct
     1801 ttgtatcact tgtatcactt gtgtctattg tttcgctcat tgttgtcagg aattttcttt
     1861 aagaatcagg gacaatgccg taaggagttg aggagtagtg cgcctgacaa cagatgcgat
     1921 gcgaaacaga gaatggcgag atggctggag tgagtgacgg tggagagcag attggatgat
     1981 gaattaacct gtccggagca gaaatattgc attgtgcaga ttctgccctt tacaggttta
     2041 gaggcatcag cgctactttc cgccgcataa gcaaatgtgc caggagcgct tatgcggcag
     2101 gaacatgtgt gatgtgttta tatggaaaat gatggactgc tttattgctc atgggttaat
     2161 gctaccatac gattttgcga tgtgtcaagc attcagagag ccacaaacaa actgagcaaa
     2221 tattgaatgc atcttgagtt ctcctagagg tttggccgct acaatgggct gtgtgggaag
     2281 aagtcccacg gatgatttgt aaacaaaacc ttgtaggaga tacaaatgaa tctgttgaac
     2341 atctcgcgac agcgggcggc gggcctcatg accatcctgc ttctcatgct gctgctggcg
     2401 gcttgcggca caccggcgaa tgccgctagc tcaggcagct cgcagggtac gagcgcacag
     2461 cagacgcagc agcgccagcc cacaagttcc aatgccacta ccagctcggc gaaaagcggt
     2521 atgcgcagta ccgatcagca agtgcagagc ttgctgcgcc agctggatgg cgcgcagggc
     2581 gatgtgaata acgccgacgc gggcgccacc caggatagtg gccaaccgta gacctcgaaa
     2641 catcagatga cgcataacta tcaacaatga aatcacctgt accatacctg tatgaaagga
     2701 tcgtcgtatg aaactcttcg taatgtttcg caaactcacc tggcgcgctg cgggcgtcgc
     2761 tgtactcgca ctcttcgcgc ttggcgcgct tcttccatca accgctttcg cggcaactgc
     2821 gtcttcgacg ccaccctgcg cctcaaccga tacgcagtgc tttatcaagc tgggcgatca
     2881 atttatcgcc gctcgtcaaa cggcgctgac aacgctgagc ggaaaagtta gcgggcgact
     2941 gaaccagcag cttattagca gcgaccaggc tgccgtgctg caggccgata tcagcactaa
     3001 tcagcagggg ctgagcgcgc tcgagacaaa gctgaacggg gagacgaacg cgctggccgc
     3061 gaagcaggat gtggtgaatg tcttcttcca gttccgcatt tacgccgtcg ttctgccgcg
     3121 tgattatcgc cgcctctacc tggatgttgc cataacggtt gatgccaaac tgcgcggctt
     3181 tggaccaaag gtgcaggaag ctatccagaa tgcccccgcg agcgagcagg cgcagctcaa
     3241 gacgctcttc tccgattatc agaaccagct ctcgacagcg gaggggcagt ttgactcggc
     3301 tgccgctgat atcgtgacgc tcatgcctga gaattt
//
LOCUS       DASZAK010001266         2983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_351073, whole genome shotgun sequence.
ACCESSION   DASZAK010001266 DASZAK010000000
VERSION     DASZAK010001266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351073"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VGT44_22255"
     CDS             <1..397
                     /locus_tag="VGT44_22255"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2583600.1"
                     /translation="PREGEWSAIDVLLHVRTIAAIDDAAITAVLERGEQPPDIEDTIV
                     PAPLETTRTELLADLERFRKQLVAVVLQADPEAHLDITWSHREFGPMQWREWLLFARV
                     HTLDHTRQIQAIAATLVSPGEGAGHESSH"
     gene            381..1448
                     /locus_tag="VGT44_22260"
     CDS             381..1448
                     /locus_tag="VGT44_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HEV2583601.1"
                     /translation="MKVAIDLGFPDVQWEPHITYTVEAERLGVDFAWSPEAWGYDAVT
                     PLAYLAAKTSRIRLGTGIMQIGARTPAMIAMTAMTMAALSGDRFILGLGVSGPQVMEG
                     WHGVRFAHPLQRTREVIDIVRMITRGERLSYAGEIYQLPLPGGEGKALISAARPRPNL
                     PIYLATLGPKNLELTGELANGWLGTSFIPEHARMFFDHIEAGATRADRSLADIDLQVN
                     AGVVDFSDDLDRLIAPRKPGLAFTLGAMGSRKHNFYNEVYQRAGYAEVALQVQDLWLK
                     RQREAAVALIPDELVLKTNLIGTKEMVRERLRTHQRAGVTTLRVSPEGQSLNERLSIL
                     GELVDLVNEVNAEASKRKAEN"
     gene            1482..1967
                     /locus_tag="VGT44_22265"
     CDS             1482..1967
                     /locus_tag="VGT44_22265"
                     /inference="COORDINATES: protein motif:HMM:NF019632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV2583602.1"
                     /translation="MPETITKAALLNNIQSGYNQFEALLAPLSEEQMTIPVVNGPWSI
                     KDNIAHLDTWQDYELNQLRGIIDGEKPQEFMPGLSEDEENEVFYQQNKDRPLAEVVAD
                     FRLSYQRILAAVQSLSEEALNAPFPWRKDGNAVWGSIAGNTYEHYEEHGTIIRRWLGQ
                     A"
     gene            complement(2018..2581)
                     /locus_tag="VGT44_22270"
     CDS             complement(2018..2581)
                     /locus_tag="VGT44_22270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583603.1"
                     /translation="MYNENPYRRPYRNAIGGVAAGIFIIGLAVAIFLGAISGHLFLPI
                     LFVGLAFASLIGAFSSYDHRAAYGGIQGFVWLLGLALCFAIGFWPWILLPIGFSMILG
                     ALAAPIMSGMRSSAFPPANQQYPPPYQTYQHGQPYQPYQWGQPYQPGQPLPPYQPVQP
                     VQPYQPAEQPSQQAPVTSQEGEHQPVQ"
     gene            complement(2714..>2983)
                     /locus_tag="VGT44_22275"
     CDS             complement(2714..>2983)
                     /locus_tag="VGT44_22275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583604.1"
                     /translation="RGEEVSDLFKHVEQVNELADGYALRFPGSDTWANTLVQFINFER
                     ECCPFFGFGLVFEQKQGPIWLHIRGPEGVKAMVEDMIRPIAERES"
BASE COUNT          706 a          828 c          838 g          611 t
ORIGIN      
        1 gccacgtgag ggagagtggt ctgccatcga tgtgctgctg cacgtgcgta ccatcgctgc
       61 aattgatgac gcggccatta cagccgtgct tgagcgcggc gagcagccgc ccgatattga
      121 ggataccatc gttcctgccc cgctggagac aacacgaacg gagctccttg ctgacctgga
      181 gcggtttcgc aagcaactcg ttgctgtcgt cctgcaggct gacccggaag ctcatctaga
      241 cattacctgg agtcatcgcg aatttggccc tatgcagtgg cgagagtggc tgctgttcgc
      301 ccgcgttcat acactcgacc acacccgtca aatccaggcg attgcggcaa cacttgtctc
      361 accaggtgaa ggagccgggc atgaaagtag ccattgacct tggtttccca gatgtccaat
      421 gggagccgca tatcacctac accgttgaag cggaacgtct cggcgtcgac ttcgcctggt
      481 ctcccgaagc atgggggtat gatgccgtca cgcccctggc ctacctggca gcgaagacca
      541 gccgcatccg cctgggaacg ggcatcatgc agatcggtgc tcgaaccccc gcgatgatcg
      601 ccatgactgc tatgacgatg gcagctctct caggtgaccg ctttattctc ggattgggcg
      661 tgagcggacc acaggtcatg gagggatggc acggtgtgcg ttttgcccat ccgctgcagc
      721 gcacgcgcga ggtgatcgac atcgtacgca tgatcacgcg tggagaacgc ctctcctatg
      781 caggcgaaat ctatcagctc ccccttcccg gcggtgaggg caaggcgttg atctcggcgg
      841 cacggccccg accgaacctg cccatctatc tcgcgacgct cggccctaaa aacctggaac
      901 taacgggaga gcttgccaat ggttggctgg ggacctcgtt cattccagaa cacgcgcgga
      961 tgtttttcga ccatatcgag gccggggcaa cgcgtgccga tcgctccctg gccgacatcg
     1021 atctgcaggt caacgcgggt gtggtcgact tctccgatga cctggatcgc ctgattgccc
     1081 cgcgcaaacc tggcctggcg ttcacgcttg gagccatggg atcaagaaaa cataacttct
     1141 ataatgaagt gtaccagcgc gccgggtacg ctgaggtggc tctccaggtg caagacctgt
     1201 ggttgaaacg ccagcgtgaa gcagcagttg ccctcatccc agatgaactg gtgctcaaaa
     1261 cgaatctgat cggcacgaaa gaaatggtgc gcgagcgcct tcgcacccat cagagggctg
     1321 gcgtaacgac gctgcgtgtt tccccagagg ggcaatccct caacgagcgt ctgagcatcc
     1381 tgggcgaact ggtggacctg gtcaacgagg ttaacgcgga agccagtaag cggaaggctg
     1441 aaaattaata tacgcccaat atagaaagag gagatacttc gatgccggaa acgataacaa
     1501 aagctgcgct gctcaacaac atacagagcg ggtataacca gttcgaagct ctgctggccc
     1561 ctttgagcga agaacagatg acgatacctg ttgtgaatgg tccctggtct ataaaagata
     1621 acatcgccca cctggatacc tggcaagact acgaactgaa tcaactgcgg ggaatcatcg
     1681 atggcgaaaa gccccaggag ttcatgcctg gactgtcaga agatgaggaa aacgaagtct
     1741 tctaccagca gaacaaagat cgaccactgg cagaagtcgt ggcagatttt cgcctctcat
     1801 accaacgcat actggccgcc gtgcagagct taagcgaaga agccctgaat gctcctttcc
     1861 cgtggcgtaa agatggcaat gctgtctggg gcagcattgc tggtaatacg tacgagcatt
     1921 acgaggagca tggcactatc atccggcgct ggttgggaca ggcataaaag agccagggca
     1981 catcctctta cgagtgccct ggtattaccg atcaaggcta ctggacgggt tgatgctcac
     2041 cttcctgaga ggttacaggc gcctgttgcg acggctgctc tgctggctgg taaggctgga
     2101 caggttgaac cggttggtac ggtggtaagg gctgacccgg ttggtaaggt tggccccact
     2161 ggtacggctg gtaaggctgg ccgtgttgat acgtctggta tggtggggga tattgctgat
     2221 tcgctggcgg aaaagctgag ctgcgcatgc cactcatgat aggtgctgcc agtgctccca
     2281 aaatcatgga aaatccaatt gggagcaaaa tccacggcca gaagccaatc gcgaagcaaa
     2341 gggcaagtcc gagcaaccag acaaagccct ggatgccgcc gtaggccgct ctatgatcat
     2401 aggagctaaa agcgccgatc agcgaagcga atgccagacc cacaaacaaa atgggcaaaa
     2461 acaagtgtcc actaatggcg cccaggaaaa tggccacggc gagacctatg ataaaaatcc
     2521 cggcagcgac gccacctatt gcatttcggt aaggtcttcg ataaggattt tcgttgtaca
     2581 tgatccgttc ctcctcccgc tacggcagta gcgattctca ctatccgata actatagaaa
     2641 cccttctata taaaggataa gatgccggag ttacggagta atcacagtgg ctcacctcgc
     2701 tatcacatga taatcacgat tctctttcag caattgggcg aatcatatcc tcgaccatcg
     2761 ctttgacgcc ctcgggcccg cgtatgtgca gccagattgg cccttgcttt tgctcgaaga
     2821 ccagcccaaa tccgaaaaat gggcagcact cccgctcgaa attgatgaat tgcacaagcg
     2881 tgttcgccca ggtgtcgcta cctggaaagc gtagggcata gccatctgcc aattcgttta
     2941 cctgctcgac gtgcttgaag aggtcgctta cctcttctcc ccg
//
LOCUS       DASZAK010001267         4407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_244775, whole genome shotgun sequence.
ACCESSION   DASZAK010001267 DASZAK010000000
VERSION     DASZAK010001267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244775"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VGT44_22280"
     CDS             <1..976
                     /locus_tag="VGT44_22280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583605.1"
                     /translation="HPPAAEKYVPSLDAWVSAGQTPQSLVLTSINQTAVEEIGPIVLL
                     PNGTAFAIGATGHTAIYMPPKADASQPGSWATGPDFPTDTSGNKLWPILTAIDAPACL
                     LPHGKVMCVAGVTYGEGQPPTYWSGPTTFFEYDPATNSFTQLANQPATNGNDTWTARL
                     LLLPTGEVLYSSQQNQLALYTPDPADGQPDPSWRPTITQLPQKMVPGQTYSISGTQLN
                     GLSQAVSYGDDAQMATNYPLVQLKNPANGAVVYLRTFNFSSLGVATGNTPQSAHVQVP
                     MGIQVGQWNLVVIANGIASDPVAIEVTDQALFIIDHTEHDRKTRWKHE"
     gene            961..1359
                     /locus_tag="VGT44_22285"
     CDS             961..1359
                     /locus_tag="VGT44_22285"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2583606.1"
                     /translation="MEARISIITLGVIDLEASIHFYQNGLGLPLREGTDGIAFFELKG
                     TWLALFPIEELAADAHVPAQRSGFSGFTLAHNVKSKEEVENVLNEARRAGAIITKAAQ
                     DTDWGGYSGYFQDPNGYLWEVAWNPFFWIE"
     gene            complement(1365..2891)
                     /locus_tag="VGT44_22290"
     CDS             complement(1365..2891)
                     /locus_tag="VGT44_22290"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEV2583607.1"
                     /translation="MRTLEVLIEQNALGAVRPMEVAAQAPISDLLPAIVEELQLPRTD
                     LFGNRLVYLLRHANNGPVLPGDRSLEAAGIDRGAQLALDSYVVDGSVAAVIRGQGTGQ
                     LYQPGFYASETMADAEALSALDLHTSAALPAVRRPKKRRWTRRAFLVMGSMALGASTV
                     GLSYAGYAAYRQYVANRQSPTSLGMMQTTATQKKPVSMPVPTSAQAALVFSQHTQPVR
                     SVVWSPDGKLLASGANDAQLFIWDTMGAVQRQQQQSGQVRAVAWSPDGTLLATAATNR
                     LTWLNAQTGAQQAQSRHTHTGTITTLDWSPQAPYRLVSGATDNTAVVWDSTAFQAQTV
                     FQGHTAPVESASWAADNQTVGTSSHGGVVRVWMASGGQELHAPFIDAQQPMRALAFNQ
                     TTNLLAVGGDDGIVRLWNGLTCQQAGQGQFGAQCLDTPTRIAAQHGVLRALAWSPDGR
                     FLASGGDDGSLALWNPAQSQVPLLTAHHNNPVLALAWSPDGKHIAAASGNTVTLWTLQ
                     "
     gene            3071..>4407
                     /locus_tag="VGT44_22295"
     CDS             3071..>4407
                     /locus_tag="VGT44_22295"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEV2583608.1"
                     /translation="MFRRIAYTGCLLLLALPLLLPSAAFALRWQSGTTANTLVPAVDP
                     DYIYSQLSYMVTQYQRREAGYDNNLPVTVNGHDEFAAYWAQEMMRDLQGFGAQMRRDS
                     FPIKGWQGRPATVAAFNVEVTVPGVTHPDQIVMIGCHYDGEAISNQSAFDDGSGCAIE
                     LGVARAMAAYWRATQTYPARTLRFVIYDAEEQGLFGSFHYLNSTINGDIPNVVAMFNE
                     EQNGIAYPLRYLGKASNPLYPFYISLAPLQNNGAYPDQSKLSPQQRAALARFRALMQQ
                     SVAPTFALFRSEGFQNLTYHTDSGQDAAQPIFTPDQVGHIALQDDPGLGSDQVPFTLA
                     GVPAATLSGNYQPDPKYPKIPGYPFDTPVDTIQLMNTFADGSSQQSQALTLALALPGQ
                     LTAWMLSQPDIVGTTTADGMPIAAIGDIGQTTVGHAVTFDADASFDPLQSGAA"
BASE COUNT          908 a         1372 c         1241 g          886 t
ORIGIN      
        1 ccatccaccg gcggcggaaa aatatgtgcc atcgctggat gcctgggtca gtgccggcca
       61 gaccccgcag agccttgtct tgaccagcat caaccagaca gcggtcgaag aaatcggccc
      121 gattgtgctg ctgccgaatg gcactgcttt tgcgattggg gccaccggtc ataccgcgat
      181 ctatatgcct cccaaggccg atgcgagcca gcctggcagt tgggcaactg gccctgattt
      241 tcccaccgat accagtggga acaagctctg gccgatcttg acggcgattg atgcgcccgc
      301 ctgtctgctg ccccacggca aggtaatgtg cgtggcaggc gttacttacg gagaagggca
      361 gccacccacc tactggtcag gtcccaccac tttttttgaa tacgatccgg ccacaaatag
      421 cttcacgcag cttgccaatc aacccgcaac aaacgggaac gacacctgga cggcgcgcct
      481 gctgctgctc ccaaccggcg aggtactcta tagctcgcag cagaaccagc tcgcactcta
      541 cacgccggac ccggcagacg gtcagccgga tccttcgtgg aggccgacca tcacacaact
      601 cccgcaaaaa atggtacctg gccagaccta ctccatctct ggcacgcaat taaatggcct
      661 atcgcaggcc gtcagctatg gagacgacgc acagatggcg accaattatc cgctcgtcca
      721 actcaagaac ccggccaacg gcgcggtcgt gtacctgcgc accttcaatt tttcgagcct
      781 gggggttgcc accggcaata cgccacaaag cgcccatgtg caggttccca tgggtatcca
      841 ggtgggacag tggaacctgg tcgtgatcgc caacggcatc gcgtccgatc ccgtcgcgat
      901 cgaagtgacc gaccaggctc tctttatcat cgaccatacc gaacatgata ggaagacaag
      961 atggaagcac gaataagcat cattaccctt ggtgtgatag acctggaagc atcgatccat
     1021 ttctaccaga acggacttgg gctgccacta cgcgagggga ctgatgggat tgcctttttc
     1081 gagctgaaag gaacgtggtt agcgctcttt ccaatcgagg aactggctgc tgacgcgcat
     1141 gttccagctc aacgatcagg tttttcagga tttaccctgg ctcacaacgt aaaatcaaag
     1201 gaagaagttg agaacgtcct gaatgaagcc cggcgcgccg gcgcaatcat cacgaaagcc
     1261 gcacaggaca ccgattgggg tggatattcg ggatattttc aggaccccaa tggatacctg
     1321 tgggaggttg cctggaatcc ctttttctgg attgaatagg tctcctattg caacgtccac
     1381 agcgtaacgg tattgcccga cgcggcggcg atatgtttgc catcgggcga ccaggccagg
     1441 gccagcaccg ggttgttgtg atgcgccgtc aagagcggta cctggctctg cgcgggattc
     1501 cagagcgcga gcgatccatc gtctccaccg ctcgccagga aacggccgtc gggcgaccag
     1561 gccagggcgc gcagtacgcc atgctgcgcc gcgatgcgcg ttggcgtatc gaggcactgc
     1621 gccccgaact gcccctggcc cgcctgctgg caggtcaggc cgttccagag ccgcacaatg
     1681 ccatcatccc cgccaacggc gagcaggttc gtggtctggt tgaaggccag cgcgcgcatc
     1741 ggctgctgcg cgtcgataaa gggcgcgtgg agttcctggc caccgctggc catccagacg
     1801 cgcacgacgc cgccatgcga tgaagtcccg acggtctggt tgtctgcggc ccacgaggcg
     1861 gattcgaccg gtgcggtatg cccctgaaaa accgtctggg cttgaaatgc ggtcgaatcc
     1921 cagacgacgg ctgtgttatc ggtcgcgcct gacaccaggc gatagggcgc ttgcggcgac
     1981 cagtccagcg tagtaatcgt gccggtatgc gtatggcgag attgcgcctg ttgcgcgcct
     2041 gtctgggcat tcagccaggt gaggcggttc gtcgcggccg tcgccagcag cgtgccatcg
     2101 ggagaccagg cgactgcacg cacctgcccc gactgctgct gctgtctctg cacagcgccc
     2161 atcgtatccc agataaagag ctgcgcatcg ttcgcgccgc tggccagcag cttgccatca
     2221 ggcgaccata ccactgagcg taccggctgg gtatgctggg aaaagaccag agcggcctgc
     2281 gcgctggtgg gaacaggcat ggaaaccggc ttcttctgcg ttgcggttgt ctgcatcatc
     2341 cccaaactcg tcggcgactg cctgttggcg acatactgcc ggtacgccgc gtagccagcg
     2401 tagctcaggc cgacggtact tgcgcccagg gccatactgc ccattaccag gaaggcgcgg
     2461 cgcgtccagc gtcgcttttt aggacgcctc acggcgggta aggcagcgga ggtatgcaga
     2521 tcgagcgcgg acagcgcttc ggcatcggcc attgtctcgg acgcataaaa gccaggctgg
     2581 tacagctgcc ccgtaccctg acctcgtatt acagccgcca ccgaaccatc cacgacatac
     2641 gaatcgagcg ccaactgcgc gcccctgtca ataccagctg cttcgaggct tctatcgccc
     2701 ggcaagaccg gcccattatt ggcatggcgc agcaaataga ccaggcgatt gccaaacagg
     2761 tccgtgcgcg gcaattgtag ttcttcgacg atggcaggca agagatcgga aatgggcgct
     2821 tgtgccgcca cttccatagg ccgcaccgcg ccaagcgcgt tttgttcgat caaaacttcg
     2881 agtgttctca cgacgtacac cccttcatac tgtgacttcc cttgccacag tgtaccatgt
     2941 tgttttattt ctattcacat ctataaggta aaatggctag caaatcgaga tgcgcaatag
     3001 ttttcttaac cacgagtctt atctggtagt agaggttttt gattgctctt tattcgaggg
     3061 agatggttcc atgtttcgcc gtatcgccta caccggttgc ctcctgctgc tcgcgttgcc
     3121 actgctttta ccatcagcag catttgctct gcgctggcaa tccggcacaa ccgccaacac
     3181 gctcgtcccc gccgtggacc cagactatat ctacagccag ctttcgtata tggtgacgca
     3241 ataccagcgg cgcgaggcag gctacgacaa caatctcccc gtcacggtga atggccacga
     3301 cgaatttgct gcctactggg cgcaggagat gatgcgcgac ctgcaagggt tcggcgcgca
     3361 aatgcggcgc gattcgtttc ctatcaaggg ctggcaggga cgcccggcaa cggttgcggc
     3421 cttcaacgtt gaagtaactg tgccaggcgt cacacatcct gaccagatcg tcatgatcgg
     3481 ctgtcattat gatggggaag ccatctccaa tcaatccgcg ttcgatgacg gcagtggctg
     3541 cgcgattgaa ctgggcgtgg ccagggccat ggcggcatac tggcgcgcca cccagaccta
     3601 cccggcacgc acgctgcgtt tcgtgattta cgatgccgag gaacagggac tgtttggctc
     3661 gttccattat ctcaactcaa ccatcaacgg cgacatcccg aacgttgtgg ccatgttcaa
     3721 cgaggagcaa aacggcattg cgtatcccct tcgttatctc ggcaaggcgt ccaatccgct
     3781 ctaccccttc tacatctcgc tcgcgccatt gcagaataac ggggcttatc ccgatcaaag
     3841 caagctctct ccgcagcagc gagcagctct tgcgcgtttt cgcgcgttga tgcagcagtc
     3901 tgtcgccccg actttcgctc tcttccgctc ggagggattc caaaacctga cctaccatac
     3961 cgacagcggc caggacgctg cgcagccgat ctttaccccc gatcaggttg gccacattgc
     4021 gctgcaggat gatcccggcc tgggcagcga ccaggttcct tttacgctgg ccggtgttcc
     4081 cgccgcgaca ctctccggca actaccagcc tgatccaaag tacccaaaga tacctggcta
     4141 tccctttgac acgccggtcg atacgatcca attgatgaac accttcgccg acggcagctc
     4201 gcagcaatcg caggcgctga cactggcgct ggcgctgccg gggcaactca ccgcctggat
     4261 gctcagccaa cctgacatcg ttggaacaac aacggctgat gggatgccga ttgcggccat
     4321 cggcgacatt gggcaaacga ccgttggtca cgctgtcaca ttcgatgccg atgcatcatt
     4381 cgatccgctt cagagcggcg ccgcgtt
//
LOCUS       DASZAK010001268         5769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_137233, whole genome shotgun sequence.
ACCESSION   DASZAK010001268 DASZAK010000000
VERSION     DASZAK010001268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137233"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            379..1041
                     /locus_tag="VGT44_22300"
     CDS             379..1041
                     /locus_tag="VGT44_22300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2583609.1"
                     /translation="MPAESVDGAVGVMSARKSYAQGAVVESLSDTAVVELVLAGDSDA
                     FATLVERYKDAVQNLAYRMLGNVTEAEDVTQEVFVRAYTQLATYKPAHKFSTWLLSIA
                     SHLSIDQLRRRRFLALPLEDVPFLEWISDLGTGPEQSALEGEQHDEIQQYLQRLPGKY
                     RAVIVLRYWYDLSYEEISQMLRLTPALVKARLHRARELLARSMKETSLNEEAQVDALP
                     QF"
     gene            1022..1459
                     /locus_tag="VGT44_22305"
     CDS             1022..1459
                     /locus_tag="VGT44_22305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583610.1"
                     /translation="MRFRNSERATSSASRPYTRISTERIMYAVEQQKRITNQLESLQA
                     QQQVQLARVGRSGTKIIGVFFLLTSAVMLGLCILFVFQPDLFLDVLSFSSGVIDIAMQ
                     LSRYLSTGLVFITGQSWLLSGVALVVVIMIGIWIRLMRAPQEV"
     gene            1498..2490
                     /locus_tag="VGT44_22310"
     CDS             1498..2490
                     /locus_tag="VGT44_22310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEV2583611.1"
                     /translation="MRHTERLLWFLLVSVGVIAFLILTTGTILSTVTPATQVVRAAGL
                     LDTTNGAYSGACTETTRSPSFGGTVVVATHDVLCSDLTVFGGTVMIHGQLQGNLLAFG
                     GTINIDGGVSGDITLFGGSVNLLTGARVHGSIHLYGGQEIRQPGAFLDGTVDDHSQHS
                     LYLGFQGPGFSVWYLLFMIPLSLLWMRFLPIHTAFVRATIEQHTRRSFLVGLLTCLLA
                     PIIVFLLLALIIAIPVALIILIGLFAAWLSGIIAVSWAIGEQVLRALTSRPVNRKSRY
                     LAVVLGQIALALFASLPVIGWFVGLGAGIIGLGAVLLSRFGTRMYGRPRQLLQV"
     gene            2569..3921
                     /gene="rlmD"
                     /locus_tag="VGT44_22315"
     CDS             2569..3921
                     /gene="rlmD"
                     /locus_tag="VGT44_22315"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV2583612.1"
                     /translation="MSIDVELTDLAYGGDAVGRYEGRVLFVPGGIPGERVRVEVVEER
                     RGHARADLLEILRPAPERIEPEFPLLNDSGCQWQHIAYPAQLTWKAHIVRQLLVRIGK
                     QPDALVHPTLGMPSGISPWHYRNIALFSVGPNNEVGFKLTESHDVQDLETCPLLHPAL
                     DLVYQRIRAKLIEKFGDSLAQMIQGFTLRGAIGAVGLNNTTTPVKAVPTLLNIHARPG
                     SVFDEPQELAQELMTIAPGIVGVVIERVGGRYGRVVAGQEFLTDMVHNKRFRVSADSF
                     FQVNMVQTHLLVERVLSMLEPMRSDVVLDGYSGVGLFSAFLAPRTSRVIAIESQPSAV
                     MDARANATLNNQMNITTLEGTLERLLGQIYYRRERIDLVVVDPPRAGCHPKAIQALQS
                     LAPRTIAYVSCDPSTLARDIASLCSTDRYRLVYAQPLDMFPQTYHIECIALLARKGLR
                     "
     gene            complement(3983..5557)
                     /locus_tag="VGT44_22320"
     CDS             complement(3983..5557)
                     /locus_tag="VGT44_22320"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEV2583613.1"
                     /translation="MSSNAPQNPQTNLWKKKRVHRVIRRFKRGPDGKLHFDGQQPDEE
                     IRRIVREHPIFSVRSALPFVVTLVFTGFFIWGNVTVPQLGIAWRLIEFLAAVALVVTG
                     AYGGYRIFELWWVNVDVVTNKRILTWHGLLSPTRKETTLDKVTQVAVDQDSIFAILVN
                     FGDVKVYLAGGKDLDLLKVSNPKEVRDDIEGIRQSYKEKLPKKAPIPTVQDAQVSEVL
                     AKLAKPEEVPKMEDADKKWEHRRDPRKLRGPLRTFGGPLRIPCEVHYDSEEYTVRYVQ
                     RSRIVLVYSEIIPVAAMIFLVFGALTFHSLLTLFVVAFFVDLLVIGLVFINYIDDVFI
                     LTNKRIIDIERKFIIFYEEHDTTTYDKVAEMTVKSPNIFELAFNIGDLYIRTPGSNPD
                     IYMKRVSDPFALQDEIYAIKGFKEKVDKVKAKNDQKDQLNEWFSTVLSKLDTRINNRG
                     VPDLQSKDLWEAMAQAGELGMRVIPIGESERYPHVPAGKIVEQSPPPGTMVSMEHSDP
                     DHKPQIHVIVSKSVTV"
BASE COUNT         1230 a         1658 c         1551 g         1330 t
ORIGIN      
        1 accgccgatt gatcggcgcg aatcttcgtc gatccggtca cttgactggc caacaatgca
       61 gctagataaa atgcctccac acccatagca gcgaggacga ggccgccctg gaagtaccgc
      121 cctcttgctt gtttttccca ctcatgttac actctttact agagattaca acttttttag
      181 ataccagtcg taatattctt gtaagctgtc tcttttctga aaaatggcca ctatacagaa
      241 aaaagaatgt aaacggtgat agatatggat acaagtctac ctgcttctac agcggtagga
      301 gtctttggta atctgtcaag gggcgcgcgg ggacgacgtg cgggggcgtt atccaactct
      361 gcgcctgcgg gacaggcaat gccagccgag tctgttgatg gggcggtcgg cgtaatgtcg
      421 gcgcgtaaga gctacgccca gggcgctgtc gtagagtcgt tgagcgatac tgcggtcgtc
      481 gagctggtac tggccggtga ctcggatgcg ttcgcaacgc tggttgagcg ctataaagac
      541 gcggtgcaaa accttgcata ccgcatgttg ggcaacgtca ccgaggctga ggatgtcacc
      601 caggaagtat ttgtgcgagc ctatacacag cttgcaacct ataagcccgc tcacaaattc
      661 agcacctggc tgctttcgat tgcctcgcat ctctcgatag accagttgag gcgcaggcgc
      721 ttcctggccc tccccctgga ggatgtgcca ttcctggaat ggatctcgga cctgggaacc
      781 gggccagagc aatcggcctt agagggcgag cagcacgacg agatacaaca atacttgcag
      841 cgcttgcctg gcaaataccg cgccgtgatc gtgttgcgct actggtatga tctctcctat
      901 gaagagatct cccagatgct gcgcctgaca ccggctctgg taaaagcgcg tttacaccgc
      961 gcccgcgagt tgctcgcgcg ctcgatgaag gaaaccagcc tgaacgagga ggcacaggtc
     1021 gatgcgcttc cgcaattctg agcgagcgac atcttccgcg tctcgcccat atacgcgcat
     1081 ctccaccgaa cgcatcatgt acgcggtgga acagcagaag cgcatcacaa atcagcttga
     1141 gagcttacag gcgcagcaac aggtacaact ggctcgtgtt ggaagatctg gcacgaaaat
     1201 cattggtgtg ttcttcttac taacatcagc tgttatgctg ggattgtgca tcctgtttgt
     1261 ctttcaaccc gatctcttcc tggatgttct ctcgttctcg agtggtgtca ttgatatcgc
     1321 gatgcaactg tcgcgctatc tctccaccgg tcttgtcttc attaccggcc agagctggct
     1381 cctatcgggg gtggctttgg tggtagtgat tatgataggt atatggattc gcttgatgcg
     1441 cgctccccag gaggtataag ggcgcgggaa tcgagaacca tacgagggag taccagtatg
     1501 cgacatacag aacgattgct ctggttcttg ctcgtatcag tgggagtgat cgcctttctg
     1561 atactgacaa caggcacgat tctttcgaca gtaacacctg caacgcaggt agtgcgagcg
     1621 gcggggctac ttgacacgac aaacggggca tattccggcg catgtacgga aacaacccgt
     1681 tcaccgtctt ttggcggaac agttgtggta gccacccatg acgtgttatg cagtgacctg
     1741 acagtctttg gtggaactgt gatgatacac gggcagttgc aaggcaatct gctcgctttc
     1801 ggcggcacaa tcaacataga tggaggtgta tcaggcgata ttacgttgtt tgggggcagt
     1861 gttaacctgt tgactggcgc gcgtgtgcat ggttcgattc atctctacgg cggtcaggaa
     1921 atcaggcagc ctggggcatt cctggatggg acggtcgatg accacagcca gcattctctc
     1981 tacctgggtt ttcagggacc tggtttttcg gtctggtacc ttttgtttat gatccccctc
     2041 agcctgctct ggatgcgctt cctcccgata catacggcct ttgttcgcgc gacgattgag
     2101 cagcacacgc ggcgaagttt cctggtcggg ctgctcactt gtctgctcgc gccgatcatc
     2161 gtattccttc ttctggcact cattatcgct attccggtag cgttgattat cttgatcgga
     2221 ttgtttgcag cgtggttatc tggcattatt gccgttagct gggcgattgg ggagcaagtc
     2281 ttgcgcgcgc tgacctctcg cccggtcaac cgtaaatcgc gctatctcgc tgttgtcctg
     2341 gggcaaatcg cgctggcgct cttcgcctcc ctgccggtta tcggctggtt cgtcggcctc
     2401 ggcgcgggca tcatcggttt gggagccgtg ctgctgagtc gattcggcac ccgcatgtat
     2461 ggcaggccca ggcagttgtt acaagtctaa caacactctg ttggaaaggt gtgacggtca
     2521 cgatagtgtt aagagaaagc taacctatca gtggacgttg aggtgaacat gagcatagac
     2581 gttgaactta cagacctcgc ctacggggga gacgccgtag gccgctacga aggacgagtc
     2641 ctttttgtgc ctggcggtat tccaggcgaa cgtgtgcgcg tggaggtggt cgaagaacgt
     2701 aggggacacg cgcgcgccga cctgctcgaa atcctgcgtc ccgcgccgga acgcatcgag
     2761 ccagaatttc ccttgctgaa cgatagcggc tgccagtggc agcacatcgc ctaccctgcg
     2821 cagttaacct ggaaagccca tatcgtccgc cagctgctgg tgcgcatcgg caagcagccc
     2881 gacgccctgg ttcatcctac cctgggaatg ccctccggca tctccccctg gcattatcgc
     2941 aatatcgcgc tcttttccgt ggggccaaac aacgaggtag gcttcaagct gaccgagagc
     3001 cacgatgtac aggacctcga aacctgcccg ctgctgcatc ccgccctcga tctcgtctac
     3061 cagcgtattc gcgcgaagct catcgaaaaa ttcggcgact cattggcgca gatgatccag
     3121 ggcttcacgc tgcgcggcgc catcggcgcg gttggcctga acaacacaac cacaccggtc
     3181 aaagccgtcc ccacgctcct gaacattcat gcccgcccag gctcagtctt cgatgagcca
     3241 caggaactgg cccaggaact catgaccatc gcacccggca tcgtcggcgt ggtgatcgag
     3301 cgcgtcggcg gtcgctacgg tcgcgtcgta gccgggcagg aattcctgac cgacatggtg
     3361 cataacaaac gcttccgcgt ctccgcggat tcattcttcc aggtcaacat ggtgcagacg
     3421 catttgctgg ttgagcgggt actctccatg ctcgaaccga tgcgctcaga tgtcgtgctg
     3481 gacggctaca gcggcgtagg ccttttctcg gccttcctgg cgccacgcac gtcgcgcgtc
     3541 atcgccatcg agtcgcagcc aagcgccgtg atggatgcgc gcgccaatgc caccctgaac
     3601 aaccagatga acatcacgac cctggaaggc accttagagc gcctgcttgg gcagatctac
     3661 tatcgtcgcg agcgcatcga cctggtcgtg gtcgatccac cgcgcgccgg gtgtcatcca
     3721 aaggccatcc aggccctgca atcactcgcg ccgcgcacta ttgcctatgt ctcctgcgat
     3781 ccaagcacgc ttgcccgcga tatcgcttcg ctctgctcca ccgaccgcta ccggctggtc
     3841 tacgcgcagc ccctcgatat gttcccgcaa acctaccata tcgagtgcat cgcgctgctc
     3901 gcgcgcaaag gactgcgata acaatctgag aagagaaaac cgccctggcc gacagccagg
     3961 gcggttttct cttctcttcc acttagacgg taacgctctt gctcacaatc acgtgaatct
     4021 gcggcttgtg gtccggatcg ctatgctcca tactcaccat cgtgccgggc ggcgggcttt
     4081 gctcaacgat tttccccgcc ggtacgtgcg gatagcgttc cgactcgcca atcggtataa
     4141 ccctcatacc gagttcgccg gcctgcgcca tagcctccca caggtccttc gactgcaaat
     4201 ccggcacgcc ccggttatta atcctcgtat ccagcttcga cagcaccgtg ctgaaccatt
     4261 cgttcagctg gtccttctga tcgttcttgg ccttgacttt atccaccttc tccttaaacc
     4321 ctttgatcgc gtaaatctcg tcctggagag caaagggatc agagacacgc ttcatataga
     4381 tatcggggtt gctgccgggc gtcctgatgt acaaatcgcc gatattgaag gccagttcaa
     4441 aaatgttggg actcttaacg gtcatttcgg caaccttatc gtaagtggtc gtatcatgct
     4501 cctcataaaa gatgatgaac ttccgctcga tatcgatgat acgcttattc gtcaggatga
     4561 acacgtcatc gatatagtta atgaacacca gtccgatcac cagcagatcg acaaaaaagg
     4621 ccaccacgaa gagcgtgagc aagctatgga acgtcaatgc cccgaacacc aggaagatca
     4681 tggctgcaac agggatgatc tcactataga cgaggacgat cctcgatcgc tggacatacc
     4741 gcaccgtgta ctcttccgag tcataatgca cctcgcacgg gatacgcagc ggcccgccaa
     4801 aagtgcgcag cggcccacgc aacttgcgag gatcgcgccg gtgctcccat ttcttatcgg
     4861 cgtcctccat tttcggcact tcttcaggtt tcgccagttt tgccagcact tcggatacct
     4921 gggcatcttg aaccgttgga atgggagctt tcttgggcag cttctcctta tagctctggc
     4981 gaatgccctc gatatcatcc cgaacttctt ttggattgga caccttcagg agatcgaggt
     5041 ccttgccacc ggccaggtag accttcacat cgccaaagtt aacgagtatg gcgaatatag
     5101 agtcctggtc gacggccacc tgcgtcacct tgtccagcgt tgtctccttg cgcgtcgggc
     5161 ttagcaggcc atgccaggtc aggatgcgct tattggtaac gacatcgacg ttgacccacc
     5221 acaactcaaa aatgcgatag ccgccgtaag cacctgttac taccaaagcg acggcggcaa
     5281 gaaactcgat caggcgccag gctatcccta actggggaac agttacgttc ccccagatga
     5341 agaagccggt aaaaactagc gtcacaacga acggcagagc tgatctgaca gaaaaaatgg
     5401 gatgttcgcg cacgatgcgc cgaatttctt catccggctg ctggccatca aaatgtaact
     5461 tgccgtctgg gccgcgcttg aaacggcgaa tgacgcgatg cactcgtttt ttcttccaca
     5521 ggttcgtctg gggattctgg ggggcgttgc tgctcatcta ctcacctcgg cttggctggc
     5581 tcggatcgat gacgatacaa agcagtaatt gtttatttct tggtcccgct ttttctaacc
     5641 ttcgtcgata tcagacccac gatcagaact acaaccccaa caaggaagag cacagggaca
     5701 ctatcggtga ggacattctg accaggagtg gcagcactga acgatacgac agaagaattg
     5761 aaaaggctc
//
LOCUS       DASZAK010001269         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_32207, whole genome shotgun sequence.
ACCESSION   DASZAK010001269 DASZAK010000000
VERSION     DASZAK010001269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32207"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /locus_tag="VGT44_22325"
     CDS             <1..1013
                     /locus_tag="VGT44_22325"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583614.1"
                     /translation="AQPKVSAEPPAAESLARGGETRRATLPLHQFRPVISIALPGRPR
                     MISWSRDGNLLISTFFSHPPLLMNRNGDTKMLPSGIARAACWNPAGRLLAVSIPTPQR
                     GQASSQAQIWDGYTPTQPLITLPLHAREIDGLDWSITGELAAWEEQQIKLFHITQRYP
                     QQAQEGITRPISTPADMTCGNTDSLRWSPDGKWLAAGAKNGAIICWHGPTRTVHHQVQ
                     SQGGFVYGLAWSPDSALLAAAFNDHHIEIWNVAEKRRVTQWRDLPHIPRALSFSIHNT
                     LAIASNTQELVLGNVRDAGPSTSHAGHWVAAWSPTRAELATLDAQTGSVLVVWEALTP
                     QS"
     gene            complement(997..2604)
                     /locus_tag="VGT44_22330"
     CDS             complement(997..2604)
                     /locus_tag="VGT44_22330"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2583615.1"
                     /translation="MQYFENAMSAQDFFANKGRAKQTRVVLLNGDIGQISPDQEADLC
                     AFVEKGGGLVCVGDAVEAYHEHPLLGELLGNVHGFCAPRSEIIARVATTDHYVTRRVD
                     PFFAVLDGIYLLDVLPPDAEVLWRASWRYASYALAYARQYGDGRVFCATPGSDAQTWE
                     HPVLRQMIERAVRFASGVETQERVRRVAMLGYGAIGFEHGTAISSVPGLEYALVCDRS
                     EERLSAARHAFPAVQTCTDLEQVAANPDIDVVIVSTPPNTHAAISMQMLRAGKHVVSE
                     KPFCLTTAEADGMIALARERELALTVYQCRRWDPDFLAIQQVIKQQAIGDIFHLETFI
                     GGFAHPCDYWHSHEPVSGGVFYDWGSHYLDWILQLIPDAVVSVRGIEQKRVWHDVTNA
                     DHSIVSLRFAGGQEAEFMHSDIAAAVKPKWYILGTRGAIVGHWRQETVKSRRWSGDLI
                     EERLALSEALPDLYVHTREISGAIHEQHLTLPEPPLYAFHRNLANHLLSGESLAVPPE
                     QSRRNIAVMEAAKRSAERDGEIVTIAV"
BASE COUNT          526 a          833 c          842 g          477 t
ORIGIN      
        1 tggcgcagcc gaaagtgtca gccgaaccac ctgcagctga gagcctggcg cgtggcgggg
       61 agacacggcg ggctacgctc ccacttcacc agtttcgacc ggtaatcagc attgcgctgc
      121 cgggacgccc gcgtatgata agctggtcgc gcgacggcaa cctgctcatc agcacgttct
      181 tttcgcaccc gccgctgctg atgaacagaa acggcgatac gaaaatgctg cccagtggca
      241 ttgcgcgagc ggcatgttgg aatccggcag ggcgcttgct ggctgtcagc atccctacgc
      301 cccaacgcgg ccaggcatct tcccaggcgc aaatatggga cggctatact cccacgcagc
      361 cgctgattac cctcccctta cacgcacgtg agatcgacgg gctggactgg tcgatcaccg
      421 gcgagctggc ggcctgggaa gaacaacaga tcaagctctt ccacatcacg caacgctatc
      481 cacagcaggc ccaggagggt attacgcgcc cgatctctac accggcggac atgacctgcg
      541 gcaatacaga ttcgctgcgc tggtctcccg atgggaaatg gctcgcagca ggagcaaaaa
      601 atggcgccat catttgctgg cacgggccga cgcgcacggt ccaccaccag gtacaatctc
      661 agggcggctt cgtgtatggc ctggcatggt cgcccgacag cgctctactc gctgcggcct
      721 tcaacgacca ccatatcgaa atctggaatg ttgctgagaa acgaagagtg acgcaatggc
      781 gagacctgcc ccatatccct cgtgcgctca gcttttccat ccacaatacg ctggcaatcg
      841 cctcaaatac gcaggaattg gtgctgggaa atgtgcggga cgccgggcct tcgaccagtc
      901 atgcgggaca ctgggtggcg gcctggtcgc ccacgcgcgc ggaactggct acccttgacg
      961 cccagaccgg cagtgtcctg gttgtgtggg aagcccttac accgcaatcg taacgatctc
     1021 cccgtcgcgt tccgccgagc gtttcgccgc ctccatcacg gcgatgttgc ggcgcgattg
     1081 ctccggcgga acggcgaggg actcgccgct gagcaggtgg ttcgccaggt tgcggtggaa
     1141 ggcgtagagc ggcggctccg gcagcgtgag atgctgctcg tggatcgcgc cactgatctc
     1201 gcgggtatgc acgtagaggt cgggcagcgc ttcggagagc gccaggcgtt cctcgatcag
     1261 gtcgcccgac cagcgacgcg acttgacggt ttcctgccgc cagtggccga cgatcgcgcc
     1321 gcgcgttccc aggatgtacc acttgggttt gacggccgcc gcgatatccg aatgcatgaa
     1381 ttcggcctcc tgtccgcccg cgaaacgcag ggagacgatg gagtggtcgg cgttggtgac
     1441 atcgtgccag acacgcttct gttcgatgcc gcgcacactg acgaccgcat cggggatcag
     1501 ctgcaaaatc cagtcgaggt agtgcgaacc ccagtcgtag aagacaccgc cggagaccgg
     1561 ttcgtgtgaa tgccaatagt cgcagggatg cgcgaaacca ccgatgaaag tttcgaggtg
     1621 gaagatatcg ccgatggcct gctgcttgat gacctgctga atcgccagga agtcggggtc
     1681 ccagcggcgg cactggtaga cggtcagagc cagctcgcgc tccctcgcca gggcaatcat
     1741 cccgtcggcc tcggcggtgg tgagacaaaa cggcttttcg gagacaacgt gttttccggc
     1801 gcgcagcatt tgcatggaga tggcagcgtg ggtattgggc ggcgttgaga cgatgacgac
     1861 atcgatatcg ggattggcgg ccacctgctc caggtcggtg caggtctgga cggcgggaaa
     1921 ggcatggcgg gcagcggaca gccgctcctc gctgcgatcg cacaccagcg cgtattccag
     1981 gccgggtacg ctgctgatcg ccgtgccatg ctcgaaaccg atcgcgccat agccgagcat
     2041 cgccacacgc ctgacgcgct cctgggtctc cacgccggag gcaaagcgca cggcgcgctc
     2101 gatcatctgg cgaaggacgg gatgctccca ggtctgcgcg tcgctgccag gcgtggcgca
     2161 gaagacgcgc ccatcgccat actgacgcgc gtaggccagc gcgtaggaag cataacgcca
     2221 ggaggctcgc cacagcacct cagcgtcggg cggcagcaca tccagcagat agatgccgtc
     2281 aagcactgca aagaacgggt caacgcggcg cgtcacataa tggtcggtag tggcgacacg
     2341 cgcgatgatc tcgctgcggg gcgcgcagaa gccatgtaca ttgccgagga gttctccaag
     2401 cagggggtgt tcgtgatacg cctcgaccgc atcgccgacg cagaccagtc cgccgccctt
     2461 ttcgacaaag gcgcatagat cagcctcctg gtcggggctg atctgcccga tatcgccatt
     2521 cagcagcacc actcgcgtct gctttgcgcg gcctttgttc gcgaagaagt cttgcgcgct
     2581 catggcgttc tcgaagtatt gcatgagatc tcctgtatgt agctgcacat ggacgcgaca
     2641 atgcgcatcg ctactgaact ttcactaatt aacgggct
//
LOCUS       DASZAK010001270         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_208158, whole genome shotgun sequence.
ACCESSION   DASZAK010001270 DASZAK010000000
VERSION     DASZAK010001270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208158"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VGT44_22335"
     CDS             complement(<1..1055)
                     /locus_tag="VGT44_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583616.1"
                     /translation="MESRQVRCQTDTHRKGAASSALMLLPVALVSLALVLLAFTISAR
                     PVLAAGAHVDVMTFDTTIDPSSLNYLTDSINIAEGDGAQALVVQINTPGGDIQSMESM
                     KIAELNSKVPIITYVSPTGAMAASAGAFVTLAGQVAAMAPGTTIGASSPVTSSGGDIG
                     STEKAKIESVLVNDMTNIQQTYHRNVPLATAMVTQAASYTDQQALSSNIVDLRATSVS
                     DLLSQLDNRTVQLVNGQVTLHTAGDTLATLNEPTTDALYGLLIDPNVVFLLFVVAVIG
                     IFVEIAHPGGILPGVTGAIALILFLFGAGSIAPNWAGLALMLLAFVLLVLDVKLTTHG
                     VLTIGAVISLVVGALLF"
     gene            1316..2506
                     /locus_tag="VGT44_22340"
     CDS             1316..2506
                     /locus_tag="VGT44_22340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV2583617.1"
                     /translation="MNILQSIGQALLTSFSMFWEILWPLILGFTLSGIVQAVVSHKAM
                     AKTLGCDGPKCLTFATLFGIASSSCSYAAVALARSIFQKGASFVSSMAFELASTNLVI
                     ELGIILIVLMGWQFAAAEFVGGILMVILIALIFRFTLTPKLVQMAKEQAAKNRVGRME
                     GHATMDMSVSGGSLISRIFSSKGFTAISHFFVMDWASVWVDIALGLLIAGALAAWVPN
                     AFWQAFFFSGNPTIAKIEGPLVGPLVSIISFVCSVGNVPLAAVLWRGGISFGGVVSFI
                     FADLIVLPVLDIYRKYYGWKVMGYILLTFYITMALAGYVVEFLFQALGIIPQNRSVAA
                     ITDGIQWNYTTVLNIIFLALAAILLVRFIRTGGAAMLRMMNQSEQEMSHMEHGDEMSR
                     MEHM"
     gene            complement(2555..>2960)
                     /locus_tag="VGT44_22345"
     CDS             complement(2555..>2960)
                     /locus_tag="VGT44_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583618.1"
                     /translation="EFDPANLMPLCRTHHLFIGHLDSWDSFNEKVMDDIATWASLMAQ
                     AQFEQDVSWTTAMQARPGAHYEQWGDAFTQHFKDLMTQWYGEQPQQDLAQLVYTWYQI
                     HIDPQELDAAASADAASTTTPENGSQVAPATN"
BASE COUNT          652 a          836 c          803 g          669 t
ORIGIN      
        1 aagaaaagca gcgcaccgac aacgagcgaa atcaccgctc cgatcgtcag cacaccatgc
       61 gtggtcaact tcacatcgag tacaagcagc acgaatgcca gtaacatcaa ggctaagcca
      121 gcccagtttg gagcaattga cccagcgccg aaaaggaaga ggattaaggc aatcgcgccg
      181 gtgacaccgg gcaggattcc gccgggatgc gcgatttcca caaaaatgcc gatcacggcc
      241 acgacgaaga gcaaaaagac cacgttgggg tcgatcaaca gcccatacag cgcatcggtc
      301 gtcggctcat tcagcgtcgc gagcgtatct cccgccgtat gcagcgtcac ctgaccattg
      361 acgagctgca ccgtccggtt atcgagctgc gagagcaaat cactgacgct ggtcgcccgc
      421 agatcaacaa tgttgctgct gagtgcctgc tggtcggtat acgaagcggc ctgtgtcacc
      481 atcgccgtcg cgagcgggac gtttcgatga taggtctgct gaatgttggt catatcgttg
      541 accagcacgc tttcgatctt ggccttttcg gtgctgccaa tatcgccccc gctgctggtg
      601 acagggctgg atgcaccgat agtcgtgcca ggcgccatag ccgctacctg gcccgccagc
      661 gtgacgaaag ccccggctga agcagccatc gcccccgtcg gcgagacata ggtgatgatg
      721 ggaaccttgc tattcaactc cgcgatcttc attgactcca tcgactggat atcgcccccc
      781 ggcgtgttga tttgcaccac cagggcctgc gccccatcgc cttcagcgat gttgatcgaa
      841 tccgtcaggt aattaagcga agatgggtct atcgtggtat caaacgtcat cacatcgaca
      901 tgagcgcctg cggcaagcac gggacgggcg ctgatagtga aggcaagcaa tacaagggca
      961 agcgaaacga gagcaacagg caagagcatc aaggcagagg atgcagcccc tttgcgatga
     1021 gtgtccgtct ggcaacgaac ctggcgcgat tccatcgtat ataacctccc ttggtaaaaa
     1081 acgacgcgcc tcaactaccc gcatacgtgc gtattgtagc atattcaagc ttgtgataca
     1141 cgcaatgacc acatgtacgc gataataacg atttgctata tcaaatatag catgttgcta
     1201 tgccattgtc aagctttttc gcgccgttga cagaattcag tcataatgat tacaataccc
     1261 ataccagggg ggtatagata ttgctgagct acatgagaaa gggagaccgt gtcgcgtgaa
     1321 catattacaa agcattggcc aggcgctctt gacttcgttc agcatgttct gggagattct
     1381 ctggccgttg atcctcggct tcaccctttc aggcattgtt caggcagtcg tctcgcataa
     1441 agcgatggcc aaaacgctgg ggtgcgacgg tccgaaatgc ctcacctttg ccacactctt
     1501 cggcattgca tcaagctcct gctcgtatgc cgccgtcgcg ctagcgcgtt cgatctttca
     1561 gaagggcgcg agcttcgtct cctcgatggc gtttgaactt gcctccacaa acctcgtgat
     1621 cgagctgggt atcatcctga ttgtgctgat gggatggcag tttgcggcgg cagaatttgt
     1681 gggcggcatc ctgatggtca ttttgatcgc cctgattttc cgcttcaccc tgacacctaa
     1741 gctggtgcag atggcaaagg agcaggccgc gaagaatcgc gtggggcgca tggaaggtca
     1801 cgcgacaatg gatatgagcg tttcgggcgg ctcgctcatc tccaggattt tcagcagtaa
     1861 ggggttcacc gccatcagtc acttcttcgt catggactgg gcttccgtct gggttgacat
     1921 cgcgcttggc ctgctgattg caggggcatt agccgcctgg gtccccaatg ctttctggca
     1981 agcctttttc tttagcggca acccgacgat agcaaaaatt gaaggccccc tggtcggccc
     2041 gctcgtctcc atcatcagct ttgtctgctc ggtcggcaac gtaccgctcg cggccgttct
     2101 gtggcgagga ggaatcagct tcgggggcgt ggtcagcttc atcttcgccg acctgatcgt
     2161 cttgccggtg ctggacattt accgcaaata ctacggctgg aaggtgatgg gctacatcct
     2221 gctcaccttc tatataacga tggctttagc cggctacgtc gtagaatttc ttttccaggc
     2281 gctcggcatc atcccccaga accgctccgt cgcggctatc acggatggca ttcagtggaa
     2341 ctacacgacg gtgctgaaca tcatcttcct ggcgctggct gccatactcc tggtgcgctt
     2401 tatccgcacc ggcggcgcgg ccatgctgcg catgatgaat caatccgagc aagagatgtc
     2461 tcacatggag catggtgatg agatgagccg catggaacat atgtaacggc aaaagccagg
     2521 cgatacgcca gaatcagatg caatcccggt aacgctaatt tgttgccgga gcgacctggc
     2581 tcccattctc tggcgttgta gtcgatgcag catcggcact cgccgccgca tccagttctt
     2641 gagggtcgat atggatctgg taccacgtgt agactaactg ggccaggtcc tgttgtggct
     2701 gctctccata ccactgtgtc atcagatctt taaaatgctg ggtgaacgca tcgccccact
     2761 gctcgtaatg cgctcctggc cgagcttgca tggcagtcgt ccaggaaacg tcttgctcaa
     2821 attgagcttg agccatcagg cttgcccacg tggcgatatc gtccatcact ttttcattga
     2881 acgaatccca actgtcaagg tgcccgatga agagatgatg cgtcctgcac agcggcatca
     2941 agttcgccgg atcaaactcc
//
LOCUS       DASZAK010001271         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_707805, whole genome shotgun sequence.
ACCESSION   DASZAK010001271 DASZAK010000000
VERSION     DASZAK010001271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707805"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..805)
                     /locus_tag="VGT44_22350"
     CDS             complement(143..805)
                     /locus_tag="VGT44_22350"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HEV2583619.1"
                     /translation="MADVSIEREAAPAASAVANPAPLGLSAFAMTTFVLSVVNAGLVH
                     SQVASVVIGLAFFYGGLCQLFVGFWEYRNGNTFGATAFTSYGAFWISFASLITPLFGG
                     QSAVGLLLGSKGAIVGDAGAGIGLYLLGWTIFTGIMAIGTLRLNGALIAVFGFLFLTF
                     LFLTFGWLSDTTTALNDTGTMINIGGWLGIITAVLAWYTAAAGVLASVNSPFKLPVFP
                     MS"
     gene            complement(1192..>2114)
                     /locus_tag="VGT44_22355"
     CDS             complement(1192..>2114)
                     /locus_tag="VGT44_22355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583620.1"
                     /translation="NNSNTSTQTIYSIQSQLLTPSSGGRTPNGTGGTTTVTPVTTPTT
                     ATTPNGTSTPTPGGTTPTSTSTPTSTPTPTNLGSTLPPGSPLPSESACASSIAYSSFE
                     PRTDNYTANHSVPTAAQIANLQPWGPLIGLDGKSDSLRKQITGNFTGTTDEILQWVAC
                     KWGIDVNIVRAQAVQESNWHQSQLGDYTTNQSLCPPGKWNGSSCYQSYGILQIKYTYF
                     KTEWPMSEQDTAFNAEFVYGWIRNCFEGWTDYLYSLTPAPGYPNYHAGDIWGCLGFWF
                     SGGWYTQGAINYIKIIQQDYNQKPWLQPGF"
BASE COUNT          487 a          524 c          621 g          482 t
ORIGIN      
        1 agaaatcttg aaagcctccg gccacatgct cgtgggaaga acagctacgc tcgctgtgac
       61 tcatgtccct gcgctcattt gatgttcaac ctgtttctag tgaccggagg ccctcaagca
      121 caactcacat tgcggagaaa acctagctca tcgggaagac cggtagcttg aatggcgagt
      181 tgacagaggc gagtacgcct gctgccgcgg tgtaccaggc caggactgca gtgatgatac
      241 cgagccagcc accgatatta atcatggtgc cggtgtcatt caaggcagtt gtagtgtcag
      301 acagccaccc gaacgtcaga aacaggaatg tgaggaacag gaagccgaat acagcgatca
      361 aggcgccgtt gagccttaac gtcccgatgg ccatgatacc tgtgaagatg gtccagccca
      421 gcaggtaaag gccgatacct gctcctgcat ccccaacaat tgcgcccttc gatccgagaa
      481 gcagaccaac agcactttgg cccccgaata ggggtgtgat cagggatgca aaggaaatcc
      541 agaacgcacc gtaggaagtg aacgcggtag cgccgaaggt gttgccgttt ctgtattccc
      601 aaaagccgac aaacaactgg cataatccgc cgtagaagaa agcaagacca atcacgacac
      661 ttgcaacctg ggaatggaca agacctgcgt tgacaacgct caagacgaac gtcgtcatag
      721 caaatgcgga aaggccaaga ggagcggggt tggcgacggc tgacgctgct ggtgctgctt
      781 cgcgttcaat tgaaacatct gccataaagg ttcccacctt tcatcaaaag actagcaaaa
      841 catgtacaaa gagtggtaca catcgggtta cgcgggcgcg tttgcctgcg tagctgcttt
      901 gattcgcgga cacccatgtc cgcaggattg gtaggacatg tcatccataa cgctatggat
      961 ggcttcatgc tcgcccgggg agagcgagaa acgcgctaca agaatgagta gctatagttg
     1021 gagaataaca ataactgcgc tgtcggatgc tcctcctttc aggtctcgat ggagtgaatg
     1081 ctgactcaac gcgcattgta tgttcgttga gtccgcttaa tcgacattat agcataaaat
     1141 tagcgatttg taagggggta tgctagttgt gatagctttg ttacttacaa attaaaatcc
     1201 aggttgcagc cagggtttct ggttataatc ctgttgtatg atcttgatgt agttgatcgc
     1261 gccctgcgtg taccagccgc cgctgaacca gaagcccagg cagccccaga tgtcgcccgc
     1321 gtgataattc ggatagcccg gcgcgggggt cagcgagtag aggtagtcag tccagccctc
     1381 gaagcagttg cgaatccagc cgtagacgaa ttcagcgttg aaggcagtat cctgttccga
     1441 catgggccac tcggtcttaa agtaggtgta cttgatctgg aggatgccgt agctctggta
     1501 gcagctactg ccgttccact taccgggcgg gcataggctc tggttggtcg tgtaatcgcc
     1561 gagctggctc tggtgccagt tcgactcctg gacggcctgc gcgcgcacga tgttgacatc
     1621 gatgccccat ttgcaggcga cccattgcaa aatctcgtcc gtggttcccg taaagttgcc
     1681 ggtgatctgt ttgcgcaggc tatccgattt accatccagg ccaatcaacg gcccccaggg
     1741 ctgcaaattc gcgatttgcg ccgccgttgg cacactgtgg ttggcggtat agttatcagt
     1801 gcgtggctca aacgaggagt aggcgatact ggaagcgcag gcgctttcgc tgggcaatgg
     1861 actgcctggc gggagcgttg atccgaggtt cgttggcgtt ggcgtcgagg tcggtgttga
     1921 tgtcgaggtt ggcgttgtgc cgcctggagt aggagtagaa gtgccattgg gcgttgttgc
     1981 tgtggttgga gtagtcacag gggtgactgt tgtcgtaccg cctgtgccgt tcggagtacg
     2041 tccaccgctc gatggtgtca gcagttggga ctgaatcgag tagattgttt gtgtcgaagt
     2101 attcgagtta tttc
//
LOCUS       DASZAK010001272         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_564498, whole genome shotgun sequence.
ACCESSION   DASZAK010001272 DASZAK010000000
VERSION     DASZAK010001272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564498"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..2166
                     /gene="thrS"
                     /locus_tag="VGT44_22360"
     CDS             376..2166
                     /gene="thrS"
                     /locus_tag="VGT44_22360"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV2583621.1"
                     /translation="MSVEAELLESVNYTPVQRMRHSAAHVMAEAVLEVFPEAKLAIGP
                     AIEDGFYYDFDLPRSLTPEDLPDIEQRMGRIVAGKYPFVRDRWPREQALEYFRAKGQD
                     YKVELIENLSDAEVGIYTQDTFLDLCRGPHVENTGQIGPFKLMKIAGAYWRGDEHRPM
                     LQRIYGTAWFTQAELDQYLWRLEEALKRDHRKLGRELKLFVLSDEVPAGVPLFLPNGE
                     MLRYQMESYVRETQEKYGYQHVWTSHLGKVKLYKTSKHWFNYRENMFPVMQGEQEASE
                     DEGYILKPMNCPHHITLFKSQMHSYREMPVRYAEFATLYRYEKAGTLTGLARVRSVTQ
                     DDAHVFLRPDQIQEEFNRAIDLTLEVFNTYGLTDYWISLSLRDPNKKEDYAGSDEVWE
                     QAEKALREAVENKGVQYREMIGEAAFYGPKTDFMVKDALGRDWQCSTVQLDFIQPINF
                     DLEYIGEDGQAHRPVMIHRAVTGSTERFMAMLIEHYAGAFPVWLAPVQAIVIPIADRH
                     IEYANQVQDALKEAGVRAEVDSRGERMNAKVRDAQLQKIPYMLVVGDKEAADNAVAVR
                     LRTNENLGATPLASFVERITGIIASKSREL"
BASE COUNT          472 a          606 c          749 g          437 t
ORIGIN      
        1 actatttact tcttccgcga tccatgttat catagctgta atcgaataat cgcagacgcg
       61 agacatgtcc ctccccggag gtttgtagag aggacgcgcc atcggatgaa agcgcgccag
      121 ccagcaagga gtgtcgatac ccctctccag tatgcgccgc taggaacttg attcgtcggg
      181 ttctaaagcg cacgggtctg accaccgtta gcggtcgttt gagcgcgtag tgcatcgtta
      241 cggcgcaaat ggtagctttg cagtgtcacg agctatgcgg aatctgggtg gaaccacgaa
      301 atgcgtgtat gccttttcgt cccaatgtgg gatggaaggg cttttttgtt gctcttaaca
      361 agaggaggtg tcccaatgtc ggttgaagca gagttgctgg aatcggtcaa ttatacgccg
      421 gttcagcgta tgcggcattc cgcggcgcat gtgatggccg aggcggtgct ggaggttttc
      481 ccggaggcga agctggccat cggaccggct atcgaggatg gcttttacta tgattttgac
      541 ctgccgcgca gcctgacgcc ggaggatttg ccggatatcg agcagcgcat gggtcgcatc
      601 gtggcgggca agtatccttt cgtgcgcgat cgctggccgc gcgaacaggc gctggagtac
      661 ttccgcgcga aggggcagga ttacaaggtc gagctgatcg agaacctgtc cgatgctgag
      721 gtgggcatct acacgcagga tacgttcctg gacctgtgcc gggggccgca tgtcgaaaat
      781 acggggcaga tcgggccgtt caagctgatg aagattgccg gggcctactg gcgcggcgac
      841 gagcatcgcc ctatgttgca gcgcatctat ggcacggcct ggttcacgca ggcagaactt
      901 gatcaatacc tctggcggct ggaagaggcg ctgaagcgcg accatcgcaa gctgggccga
      961 gaactcaagc tcttcgtgct tagcgacgag gtgcctgccg gtgtgccgtt gttcctgccg
     1021 aatggcgaga tgctgcgcta ccagatggag agctacgtgc gcgagacgca agagaagtac
     1081 ggctaccagc atgtctggac gtcgcacctg ggcaaggtca agttgtacaa aacgtcgaag
     1141 cactggttca actatcgcga gaacatgttc ccggtgatgc agggcgagca ggaggcgtcg
     1201 gaggatgaag ggtatatcct caagccgatg aactgcccgc atcacatcac gctgttcaag
     1261 tcgcagatgc acagctatcg cgagatgccg gtgcgctacg ccgagtttgc cacgctctac
     1321 cgttacgaga aggctgggac gctgacgggc ctggcgcgcg tgcgcagcgt gacgcaggac
     1381 gacgcgcacg tgttcttacg ccccgaccag attcaggagg agttcaaccg cgccatcgac
     1441 ctgacgctgg aggtcttcaa cacgtatggt ctgacggact actggatctc gctttcgctg
     1501 cgtgatccaa acaagaaaga ggattacgct ggcagcgatg aggtatggga gcaggccgaa
     1561 aaagcgctgc gcgaggcggt cgagaacaag ggcgtccagt atcgcgagat gatcggcgag
     1621 gcggccttct atggccccaa gacggacttc atggtcaagg atgcgctggg ccgtgactgg
     1681 caatgctcga cggtgcaact cgacttcatc cagccgatca acttcgacct ggaatacatc
     1741 ggcgaggatg gccaggcgca tcgcccggtc atgatccatc gcgctgtgac gggttcgacg
     1801 gagcgcttca tggccatgct gatcgagcat tacgcggggg ccttcccggt ctggctcgcg
     1861 ccggtgcagg ccattgtgat tccgattgcc gaccggcata tcgagtacgc caaccaggtg
     1921 caggacgcgc tgaaagaggc tggtgtgcgc gcggaggtgg atagtcgcgg cgagcgcatg
     1981 aacgccaaag tgcgcgacgc gcagctgcaa aagatcccct atatgctcgt agtaggcgac
     2041 aaagaggcgg cggacaacgc cgtcgccgtg cgcctgcgca cgaacgagaa tcttggcgcg
     2101 acgccgctcg ccagctttgt cgagcggatt acggggatca tcgcgtcgaa gagccgggag
     2161 ttgtaaggga cgcgagcggc gggctggaat ggataagggc attggcgtca agttaaggcc
     2221 gaggggactt agccaccagg gcgaccgcca gggtgcgccc ctac
//
LOCUS       DASZAK010001273         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_423483, whole genome shotgun sequence.
ACCESSION   DASZAK010001273 DASZAK010000000
VERSION     DASZAK010001273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423483"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VGT44_22365"
     CDS             complement(<1..790)
                     /locus_tag="VGT44_22365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV2583622.1"
                     /translation="MNRRSRLFLSIGIPVLLIVVVVGAFLWQQRAAGSGVSPGVAQVT
                     QTPGSECASIPKSAPTQLTTMSLALDWTPNTNHTGIYVALSQQWYRDEGINLSLLPYS
                     ASVSPDALVSSGKADVGISATEGIVTDAALGQPVVSIAAIIQHNTSALVTLASSGLTR
                     PRDLDGKTYGGFGAPYESAVVGEIIKKDGGTGQFKNVTLSVDAMQALESHRIDFVWVF
                     EGWEVIQAQRDGVKLNVFPITNYGVADYYTPNLITSPAEIQQKPD"
     gene            complement(803..1570)
                     /locus_tag="VGT44_22370"
     CDS             complement(803..1570)
                     /locus_tag="VGT44_22370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583623.1"
                     /translation="MTLRRLAGYGPALVLAITLLALWQLYAQISQISPRFLPSPVAVV
                     AALVNNWPIIYENTVPTLLETVLGIAIATLLGLFLAVLLNVSGWMRRAVYPLLVTSQT
                     IPMVALAPLLLIWVGYDISSKLIVVVLYCFFPIAVACADGLASVEPEMIRLMRSMKAS
                     RWQILWMVRLPAAMPAFFSGLRIAATYSVVGAIFGEYVGAEKGLGIYMQRATNSFATA
                     QVFAAIVVTAALSLALFGLVSLIERIALPWYHAAQRD"
     gene            complement(1567..2337)
                     /locus_tag="VGT44_22375"
     CDS             complement(1567..2337)
                     /locus_tag="VGT44_22375"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2583624.1"
                     /translation="METPKLVARDISKTFRQGRQALETLAAIDLAVDAGEFVTVIGPS
                     GCGKSTLFNIIAGIETPDTGSIAIDGEEGVERAGKAGYMPQQPLLLPWKTVEENVMLG
                     LDVRHEPRQAALAQARELLKRFGLAEFAHYYPAALSGGMRQRVALLRTVLFNRSFLLL
                     DEPFGALDALTRLASQMWLLDVWQEFHSSVLFITHDVREAILLSNRIYVLSARPARVL
                     RVLNVNLPYPRRRELLASAEAAQLEQELLDLLLKEQTL"
BASE COUNT          531 a          784 c          750 g          435 t
ORIGIN      
        1 ggtctggttt ctgctgaatc tccgccgggc tggtgatgag attgggcgtg tagtagtcgg
       61 ccaccccgta gtttgtgatg ggaaagacgt tcaacttcac cccatcgcgc tgcgcctgga
      121 tgacctccca gccctcgaag acccacacga aatcgatgcg atgcgactcc agcgcctgca
      181 tcgcgtccac gctcaacgtc acattcttga actggccggt gcccccatcc ttcttaatga
      241 tctcgcccac aacggcagac tcgtagggcg cgccgaagcc gccgtaggtc ttgccatcca
      301 ggtcgcgcgg gcgcgtcaga ccggaactgg ccagcgtgac cagcgccgag gtgttatgct
      361 gaataatcgc ggctatcgag acgaccggct ggcccagcgc ggcatcggtg acgataccct
      421 ccgtcgcgct gatgcccacg tcggccttgc ccgaactgac cagcgcatcc ggcgaaacgc
      481 tggcagaata gggcagcagg ctcagattga tgccctcatc acgataccac tgctgcgaca
      541 gcgcaacgta gatgccggta tggttggtat tgggcgtcca gtcgagcgcg aggctcattg
      601 tggtcaactg tgttggcgca cttttgggga tcgaggcgca ctcagacccc ggcgtctgcg
      661 tcacctgcgc cacgcccggc gacacccccg acccggcagc gcgctgctgc cagaggaacg
      721 cacccaccac aacgacgatt aacaatactg gaatgccaat gctcaaaaac aggcgtgaac
      781 gacgattcat agaaaacctc ctttaatcgc gctgcgcggc atgataccag ggcaatgcga
      841 tgcgttcgat cagcgagacg agaccgaaga gcgccaggct caaagctgcc gtcacgacga
      901 tagcagcaaa gacctgcgct gtcgcgaacg aattggtggc gcgctgcatg tagataccca
      961 gccccttttc cgcgccgaca tattccccga agatcgcgcc caccacgctg taggtagcgg
     1021 cgatgcgcag gccggagaag aaggcgggca tggcggcggg caggcgcacc atccacagga
     1081 tttgccagcg cgacgctttc atgctgcgca tcaggcgaat catctccggc tcaaccgagg
     1141 ccagcccatc ggcgcaggcg acggctatcg gaaagaaaca gtacagtaca accacgatca
     1201 gcttcgatga gatatcgtag ccaacccaga tcagcagcag gggcgcaagc gcaaccatcg
     1261 ggatggtctg cgatgtgaca agcagcggat agacggcgcg acgcatccag cctgagacgt
     1321 tcagcaggac cgccaggaac aggccgagca gtgtggcgat ggcgatgccc agcacggttt
     1381 cgagcagggt cggcaccgta ttctcgtaga tgattggcca gttattcacc agcgcggcaa
     1441 cgaccgctac cggcgatggc aggaaacgcg ggctgatctg gctgatctgc gcgtagagtt
     1501 gccacaacgc cagcaatgtg atggccagca cgagcgccgg gccatacccg gcaagtctgc
     1561 gaagtgtcat agcgtctgct cctttaatag caggtcgagc agctcctgct ccagctgcgc
     1621 cgcctcagcc gaggcgagta gttcgcgccg gcgaggatag ggcaggttga cattgagtac
     1681 gcgcagcaca cgcgccgggc gcgcgctcag cacgtaaatg cgattcgaga gcaggatggc
     1741 ttcgcgcaca tcgtgcgtga taaacaggac gctcgaatga aactcttgcc agacatccag
     1801 cagccacatc tggctcgcca ggcgtgtgag agcgtccagc gcgccaaacg gctcgtcgag
     1861 caggagaaac gagcggttaa atagcacggt gcgcagcagg gctaccctct ggcgcatccc
     1921 gccagagagc gctgccgggt agtagtgcgc gaattccgcg aggccaaagc gtttgagcag
     1981 ttcgcgtgcc tgggccagcg ccgcctggcg cggttcgtga cgcacgtcaa gacccagcat
     2041 gacattctcc tccaccgtct tccagggcag caggagcggc tgctgaggca tatagccagc
     2101 tttaccggcg cgttcgacac cctcctcacc atcaatggcg atgctgcctg tatcaggcgt
     2161 ctcgatgccc gcaatgatat tgaacagcgt gcttttgccg cagccactgg ggccaatcac
     2221 ggtcacaaac tcgcccgcgt ccacggccag gtctatcgcg gccagcgttt ccagagcttg
     2281 ccgcccctgc cgaaatgttt tcgagatgtc acgcgccacc aactttggtg tctccataga
     2341 cgtttcctac ctgagcaaga gagcaacaaa aagaccgtac cctgcgtgag agatacggcc
     2401 tgtgctgcgt cctggcgcgt gtagccctgg ttagaactgg cgcgcgcttg ccgcttccct
     2461 ccgctggtac taaccagatc aggttcatag ggtcagcagc
//
LOCUS       DASZAK010001274         4515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_32336, whole genome shotgun sequence.
ACCESSION   DASZAK010001274 DASZAK010000000
VERSION     DASZAK010001274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32336"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VGT44_22380"
     CDS             complement(<1..596)
                     /locus_tag="VGT44_22380"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEV2583625.1"
                     /translation="MEQDEQASWSLDTLLVHGSTKDAMTDHSGRPTVEPIYHSTTYLH
                     RDVETLDQAFNGQLPGGETSYVYARQGNPNAQSLENLLAEIESGVGAVVFASGMAAIY
                     AALLSAGLTTGAKILASQDLYGPTIGMIRTQFAPIGVQVALRDLCSPDVADVIREEQP
                     DVVFVETLSNPLVKLTDLDAISVTAQEVGAATIVDSTFT"
     gene            complement(654..1520)
                     /locus_tag="VGT44_22385"
     CDS             complement(654..1520)
                     /locus_tag="VGT44_22385"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2583626.1"
                     /translation="MTTQDNQPKSGNVYFTNPESSVEMARLLDQDRLITEGMGGLLAE
                     FDDLSRFHRVLDIACGPGGWAQELAIAHPEIEVVGFDISQVMIDYARAFAQVRKLPNL
                     TFHVMDLQKPLQFDDNSFDLVNSRFINFLPAAAWPKLMQELNRITKPGGVIRMTESEW
                     WYYSNSPALESLNALLIRALKAQGGSFSQSGRFTGVLPMLGQFLQEIGLINIQMKSHA
                     IDYSYGTKAGIGFQQDARANFKLFQPFIIRMGVAKQDELDQLYPQMLSEMASEHFRGL
                     MFPFTIWGEKPQ"
     gene            1830..2870
                     /gene="tdh"
                     /locus_tag="VGT44_22390"
     CDS             1830..2870
                     /gene="tdh"
                     /locus_tag="VGT44_22390"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF003808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEV2583627.1"
                     /translation="MNGKMKAALKEEPAPGATLALVDIPTIGSRDVLVQVKAASICGT
                     DVHIYQWDAWAQHRMTTPRIFGHEFAGIVVEVGHEVTQLVPGDFVAAETHIACGHCYQ
                     CQTGQAHICRNVKILGVDRDGAFAEYIAIPESVAWKTGKEIDHRVASIQEPFGNAVHA
                     TFAGEIAGKTVAVFGCGPIGLWAVGLARISGSSTIFAIEPNHKRLDIAVDMGADYLLN
                     PRDVDPVQKILDVTGGLGVDVVLEMSGHPGAIRQGFKALRNGGRVSLLGIPSSMVELD
                     LADEIIFKGATVLGISGRRIFDTWYRTRRILEAGQLDLQRVITHTLPFEQIHDAMEIM
                     QSGDCGKIVLTF"
     gene            3004..4182
                     /locus_tag="VGT44_22395"
     CDS             3004..4182
                     /locus_tag="VGT44_22395"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEV2583628.1"
                     /translation="MDKLQAFVKGELDTLREKGIFSLPRVLESEQKATVIIDGREVIT
                     LSSNNYLGLTVHPRLRAAAIKAIEDYGVGSGSVRTIAGNMTLHNVLEEKLARFKHTEA
                     SLTIQSGYATNLGVISALMQEGDIIVSDELNHASIIDGIRLNRTPRKVYPHKDMAGLR
                     RALEEAKGANKVMVVTDGVFSMDGDIAPLPEIVALAEEYGAFVMVDDAHASGVLGKHG
                     RGTISHFGLEGRVALQIGTLSKGIGALGGYVACSQDVKDYLLQRARPVLFSTSHPPSV
                     VATCIAALDLLEEDNSLVERLWENTAFFKRGLEQLGFNTGKSETPITPVIVGEGALAL
                     RLSRRLFDEGVFAQGIVFPTVPADKCRVRTIVTALHTREELSKALSAFEKVGKELGII
                     "
BASE COUNT          944 a         1324 c         1306 g          941 t
ORIGIN      
        1 gtgaaggtgc tatcgacgat ggtcgcggcc cccacctcct gcgcagtcac gctgatggca
       61 tccaggtcgg tgagcttgac gagcgggttc gagagcgttt ccacaaagac aacatcgggc
      121 tgctcttcac ggatgacatc ggccacgtcg ggactgcaca ggtcgcgcag cgccacctgc
      181 acgccaatgg gcgcaaattg tgtgcgtatc atgccaatcg ttgggccata caggtcctgc
      241 gacgccagga tttttgcccc cgtcgttaaa ccggctgaga gcagcgcggc atagatagca
      301 gccattccag aggcgaacac gacggctcca accccacttt cgatctcggc cagcagattt
      361 tcaagcgatt gcgcgttcgg gttgccctgc cgcgcataga catacgatgt ttcgccgccg
      421 ggcaactgcc cgttaaacgc ctggtcgagc gtttctacat cgcgatgcag ataggtggtg
      481 ctgtggtaaa tcggctcgac cgttggcctg ccagaatggt ccgtcatagc gtctttcgtg
      541 ctgccatgca ccagcagtgt atcgagcgac caggatgctt gttcgtcttg ttccaatgaa
      601 atcctccaga actatgagtg agcgatcttc ccacgtcttc ttccacgaat accttactga
      661 ggcttctctc cccatattgt aaaggggaac atcaagccgc ggaagtgttc gcttgccatt
      721 tcggagagca tttgcgggta gagttgatcc agttcgtcct gcttggcgac gcccattcgg
      781 atgatgaacg gctggaacaa cttgaagttg gctctggcat cttgttgaaa ccctataccg
      841 gcctttgtcc cgtaagagta atcgatggcg tgtgatttca tttgaatgtt gatgagtcct
      901 atctcttgta ggaattgccc cagcatcggc agaacgcccg taaagcgccc gctttgcgaa
      961 aaactgcctc cctgcgcctt gagcgcgcga atcagcaggg cgttcaggct ttccagtgcc
     1021 gggctatttg aatagtacca ccattcgctt tccgtcatgc ggatgacccc gcccggtttc
     1081 gtgatgcggt tcaactcttg catcagcttc ggccaggcag cagcagggag aaagttgatg
     1141 aagcgggaat taaccaggtc aaacgagttg tcgtcgaatt ggagcggttt ttgcaagtcc
     1201 atcacatgga acgtcagatt gggcagttta cgcacctgcg cgaaggcgcg tgcgtaatcg
     1261 atcatgacct gactgatatc aaaccccact acctcgatct cagggtgggc gatggccagc
     1321 tcttgcgccc agccgccagg cccgcaagcg atgtccagca cgcgatgaaa cctggaaaga
     1381 tcatcaaact ccgcgaggag gccgcccata ccctcggtga tgaggcggtc ctggtcaagc
     1441 aatcgcgcca tttctacgct gctctcagga ttcgtaaaat acacgttgcc gcttttgggc
     1501 tgattgtctt gcgtcgtcat tttgtgcctc tcctccttaa aatgatctgt aggggcgcaa
     1561 tgaattgcgc cctggtgatg ggctagaaca gggtccaggg cgcaattcat tgcgccccta
     1621 cagatcattt gagccttgat agtctagttg tcttgcggat tgcctgtcgc gctttgtaat
     1681 ccctatctgt cttgtgtgat aggatagcag ccactacaac ggctgtcaac cgcctggctt
     1741 gcattcgttc tgcggaaaac gcataataac gatagcgcat tcagcaacgt ggctgatgca
     1801 atcgtatcat catggaggat gtgacgagca tgaatggaaa aatgaaagca gccctcaagg
     1861 aggagccagc gcctggggca acgcttgccc tggtggatat ccctacaatc ggttcgcgcg
     1921 atgtgctggt gcaggtcaag gcagcctcaa tctgcggcac ggatgtccat atctatcaat
     1981 gggatgcgtg ggcgcaacat cgcatgacca cgccgcgtat cttcggccac gagttcgctg
     2041 gcattgtcgt cgaggttggc cacgaggtaa cacaactcgt tcccggcgat tttgtcgcgg
     2101 cggaaacgca tatcgcctgc ggtcactgct accagtgcca gacggggcag gcgcacatct
     2161 gccgcaacgt aaaaattctc ggcgttgacc gcgatggcgc gtttgccgaa tacattgcca
     2221 tccccgaaag cgtcgcctgg aaaacgggca aggaaatcga tcatcgcgtt gcctctattc
     2281 aggagccgtt cggcaacgcg gttcacgcca ccttcgcggg tgaaatcgcc gggaagacgg
     2341 tggcggtctt cggctgcggc cccattggcc tgtgggcggt cggactggcg cgcatctctg
     2401 gctccagcac gatcttcgcc atcgaaccca accataaacg cctagatatc gccgtcgata
     2461 tgggcgcgga ctaccttctc aatccgcgtg acgttgaccc ggtgcagaag atacttgatg
     2521 tcaccggcgg cctcggcgtt gatgtcgtgc tggaaatgtc cggccatccc ggcgcgatcc
     2581 ggcagggctt caaggcgttg cggaacggcg ggcgcgtctc gctgctgggc atcccttcca
     2641 gtatggttga gcttgacctg gccgacgaga tcatcttcaa aggcgcgacg gtgctgggca
     2701 tctccggtcg gcgcatcttc gatacctggt atcgcacgcg ccgtatcctc gaagcgggtc
     2761 agctcgacct gcagcgagtc atcacacata ccctgccttt cgagcagatt cacgacgcaa
     2821 tggagataat gcagtccggc gactgcggga aaatcgtctt gacattctag agtcgagtgt
     2881 caccctgagc gcagcgaagg gtctgatgcg ccggggaccg agatccttcg ctgcgctcag
     2941 agcttgccct gaacgaagtg aagggatgac aggctcggtc gaaaaaaggg agtttaacat
     3001 agcatggata aactgcaagc ctttgtaaaa ggcgaactgg acacgctgcg cgaaaaaggc
     3061 atcttcagcc tgccgcgcgt actggagtcg gagcaaaagg ccaccgtcat catcgatgga
     3121 cgcgaggtca ttacgctctc ttccaataac tacctcggcc tcaccgtgca tccacgcctg
     3181 cgcgccgctg ccatcaaagc catcgaggac tacggcgtcg gttccggctc ggtacgcacc
     3241 atcgctggca acatgacgct gcacaacgtc ctcgaagaga agctggcgcg tttcaagcac
     3301 accgaagcct cgctgactat ccagtccggg tacgccacca acctgggcgt catctccgcg
     3361 ctgatgcagg agggcgacat catcgtcagc gacgaactga accacgccag tatcatcgat
     3421 ggcattcgtc tcaaccggac gccgcgcaag gtctacccgc acaaggacat ggcgggcctg
     3481 cgccgcgcgc tggaggaagc gaagggcgcg aacaaggtga tggtcgtgac agacggcgtc
     3541 ttcagcatgg acggcgatat cgcgccgctg ccggagattg tcgcgctggc cgaggagtac
     3601 ggcgcgttcg tgatggtcga tgatgcccac gccagcggcg tactgggcaa gcatgggcgc
     3661 ggcaccatca gccatttcgg gctggaaggg cgcgtggcgt tgcaaatcgg cacgctctcg
     3721 aaaggtatcg gcgcgctggg cggctacgtc gcctgcagcc aggacgtgaa ggactatctc
     3781 ttacagcgtg cgcgtcctgt cctgttcagc acctcgcacc cgccctcggt cgttgctacc
     3841 tgcatcgcgg cgctcgatct gctcgaagag gacaacagcc tcgtcgaacg cctgtgggag
     3901 aataccgcct tcttcaagcg cggcctggag cagctcggct tcaataccgg caagagcgag
     3961 acgcccatca cgcccgtcat cgtcggcgag ggcgcgctgg ccctgcgcct cagccgccgc
     4021 ctcttcgacg aaggcgtctt cgcccagggc atcgtcttcc ccaccgtccc cgccgacaag
     4081 tgccgcgtgc gcaccatcgt gacggcgctg cacacgcgcg aggaactttc gaaggcgctg
     4141 agcgcgtttg agaaggtcgg gaaggagttg gggatcattt aaccgcgact ggcctggctt
     4201 ctctcaaata gaggacgcaa accactcgat tgattccgaa ggcgtgtgta cccaattatg
     4261 atttttcacg accagcatcg gtgctgccat cctgagcgca gcgaaggatc tcacccgccg
     4321 ggtcccgaga tccttcgctg cgctcaggat gacagcaccg atcttgcttg tgaaaggtca
     4381 ttattagttc aagatcaggc gcgagcgttg gaaactcacc agcgacactc caagcaagat
     4441 tgcatccagc accgcaataa atgccataat aatgagccaa aactgctgat cggttagcga
     4501 cgcgacgaag ctcgc
//
LOCUS       DASZAK010001275         2179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_282007, whole genome shotgun sequence.
ACCESSION   DASZAK010001275 DASZAK010000000
VERSION     DASZAK010001275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282007"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="VGT44_22400"
     CDS             <1..793
                     /locus_tag="VGT44_22400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEV2583629.1"
                     /translation="VFPHEVAGFSGGAKYLFPGIAGPDIINFTHWLGALATSMRTIGV
                     KDTPVRRVITQAAGFLTRPLICLAMVMQGQELHGLYIGSHTEAWSHAADLSAQLDITY
                     VDRPFRRVLSAPAEMYDDLWTAAKAMYKTEPAIADGGEVIIYAPHITEVSYTHGKLID
                     EVGYHVRDYFVKQWDRFKHVPGSILAHSTHVKGTGTYDAASGIETPRISVTLATGIPE
                     ERCRRINLGYADYREIDPEAWKGREQEGLLYVPHAGEMLYRARGI"
     gene            813..>2179
                     /locus_tag="VGT44_22405"
     CDS             813..>2179
                     /locus_tag="VGT44_22405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583630.1"
                     /translation="MTNDNTIRSLLQQLIEDDAVGQLRRRDGLMATLGSEGSALKLDW
                     VEGVARLLADPAMLEEVEVEARDIWDRGIRHLIWAGMGGSVLTVRVLCELGFGGSHDN
                     GQVTIYPLDSTDPAALNTIVRKIVTAKNLAWPSGEEASNPTFLRALLNDVMMVGVAMG
                     MTSEEPITHLEWFTDLLKRAGLRPAEHLLVMTLPDSYLDRFAHEQQAPSRPLQPDGGT
                     GTGGRMSAPATRVFLLPAALYLITHFPGQSGQLRSVLQQAWDEYNLELATSHPAEHPF
                     VQLAVALSAASLDGACRLLLGMPEGWEIFVSWLEQLMEESLGKEGKGVVVFNDQILNH
                     AAPGYQEEGMLHARVVTDITQTGDDRIFVLLQPSLASKEPQDRLAALAASFLGWQLSM
                     ALYGYLQRITFAGQPAVENYKARARALRTQADPLQVTSNWSPSLQDGILTLLAPHGAV
                     DQDTQS"
BASE COUNT          482 a          616 c          628 g          453 t
ORIGIN      
        1 tgtctttcct cacgaagtcg ccggcttctc cgggggggcc aagtatctct tccctggtat
       61 cgctgggccg gatattatca atttcaccca ctggctagga gcgctggcga ccagtatgcg
      121 caccatcggc gtcaaggata ctcctgtccg gcgcgtcatc acccaggcgg caggattttt
      181 gacacgcccg ctcatctgcc tggctatggt gatgcaaggc caggaactgc acggcctcta
      241 catcggctcc cacacagaag cctggagcca tgcagcagat ctctcggcac agctcgacat
      301 cacctacgtg gaccgccctt ttcgcagggt gctctcagca ccagccgaga tgtacgatga
      361 cctatggacg gcggccaagg cgatgtacaa aaccgaaccg gcgattgccg acggaggcga
      421 agtcattatc tacgccccgc atatcacgga ggtctcctat acgcacggta aactgatcga
      481 cgaggtcggc taccatgtca gggattattt cgtcaagcag tgggatcgct ttaaacacgt
      541 gccgggaagc attctggcac atagcactca cgtgaaggga acgggtacct acgacgccgc
      601 gagcggcatc gaaacgccgc gcatctcggt cacgcttgcc accggcattc cggaggaacg
      661 ctgccggcgc atcaacctgg gttatgccga ttatagagag attgaccctg aagcatggaa
      721 aggacgcgaa caggagggat tgctctacgt gccacacgcc ggtgagatgc tctaccgcgc
      781 tcgtggaata taaggggaag gagttttcac ccatgacgaa tgataacacc atacgatctc
      841 tcctacagca actgatcgag gatgatgcag tcggccagtt gcggcggcgc gatggcctga
      901 tggcaacttt aggcagtgaa gggagtgcgc tcaaactcga ttgggttgag ggtgtagcac
      961 gcctgcttgc tgatccggca atgctggagg aagtcgaggt agaggcacga gacatctggg
     1021 atcgcggtat tcgccacctg atctgggctg gtatgggtgg ctcggtcctt acggtgcgag
     1081 ttttatgcga gcttggcttc ggtggcagtc acgacaatgg gcaggtgact atctacccgc
     1141 tggacagcac cgatcccgca gcacttaaca caattgtgcg aaagatagta acagccaaga
     1201 atctggcctg gccctccggg gaagaagcat ccaatcctac tttcttgcga gcgcttctca
     1261 atgatgtgat gatggtcgga gtagcgatgg gtatgacctc cgaagagcca atcacgcacc
     1321 ttgaatggtt caccgatctt ttgaaacggg cagggttacg cccggcagag catttactgg
     1381 tgatgaccct accagactcc tatcttgacc gctttgcgca tgagcagcag gcacccagtc
     1441 gacctttgca accggacggg gggacaggaa caggtggacg catgagcgcc ccagcaacac
     1501 gcgtgtttct gctccccgcg gccctctatc ttattaccca tttccccggt caatccggtc
     1561 aattacggtc agtgctgcaa caggcatggg acgagtataa ccttgaactg gcaacatctc
     1621 acccggcaga acaccccttt gtgcaattgg ccgtcgccct gagtgccgcc agcctcgatg
     1681 gagcttgccg cttgctgctg gggatgcccg agggctggga aatctttgtg tcctggctag
     1741 aacaattgat ggaggagagc ctgggcaaag aaggcaaggg tgtcgtggtc tttaacgatc
     1801 aaatcctgaa ccacgcggct cccggctatc aagaggaagg catgctgcac gcacgcgttg
     1861 tgactgatat aactcaaaca ggtgatgacc gaatctttgt cctcctccaa ccctcccttg
     1921 cgagtaaaga accgcaagat cgactagcgg cgctagccgc cagttttctt ggctggcagt
     1981 tgagcatggc tctctatggt tatctacagc gtatcacgtt cgcgggtcag cctgcggtcg
     2041 agaactacaa ggcgagagcg cgcgctttac ggacgcaggc cgatcctttg caggtcacct
     2101 cgaattggag cccgtcgttg caggatggaa tcctgacgct actcgctcct catggggccg
     2161 tagaccagga cacccaatc
//
LOCUS       DASZAK010001276         9395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_707938, whole genome shotgun sequence.
ACCESSION   DASZAK010001276 DASZAK010000000
VERSION     DASZAK010001276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9395
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707938"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VGT44_22410"
     CDS             <1..206
                     /locus_tag="VGT44_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583631.1"
                     /translation="PNCWAGRSIGGECGKPAPTEVWLYSCSWFSTAQQMLPIATLKCR
                     RDTGSALAPGAAHSLQPNPPVIM"
     gene            complement(674..3106)
                     /locus_tag="VGT44_22415"
     CDS             complement(674..3106)
                     /locus_tag="VGT44_22415"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2583632.1"
                     /translation="MQDQARVVIIGSGIAGSSIAYHLAERGWRDVVVLEQGPLIGGTT
                     SHAPGLVGQLRSSVSLTKMLVYSVSLYKTLGVDGKAGYAGGSSLRLASSKERMEELKR
                     QVGFSKSVGLEAELISASEAKAMFPMMSLQGVEGALWLPTDGSARAPILAQALADRAR
                     EGGVTFHPHTRVTGIEVVNGRVQAVNTSRGRIRTETVVVAAGIWSPRIGRMAGVTIPL
                     IPMQHQYAQTAPLPELSGGLRVPNLRDPDNLVYFRQDGDSLVLGGYERDPLPLDVDDI
                     PAGDNPTVLPFDPPRFESLLRGCVERLPMLREVELTRRVNGLESFTPDGEFILGEAPD
                     VRGFWAACGFCAHGVSGSGAVGKMMAEWIIDGEPSLDLWHMDIRRFGAYTASRRYIAT
                     RVNEVYSTYYDIAYPMQERSSARKLRLSPVYSRLEELQAVFGEKAGWERPNWFASNES
                     LAEGQGWPAPYGWARRYWSPAIGAEHQATRERVALFDETSFSKIEILGPGALAFLQRI
                     TDNQMDQPVGSITYTQMPNPRGGIECDLTVTRLAADRFQIVTGTAFGNHDLTWIRSHA
                     PTDGSVYLNDITSSRCCLGVWGPRARDLLQSVSEDDFSNAAFPYLTAQYVTLGDIPAL
                     ALRVTYVGELGWEIYAPMEYGLKLWDTLWQAGQALGIVAAGYRAIESLRLEKGYRYWS
                     ADIHSEYNPYEAGLGFAVKLQKGDFIGRDALERIKAEGVKRKLCCLVLDDPAAVGLGG
                     EPFLDGERVLGHVTSAGYGYTMRQSIACGYLPVEYAAVGMRVDVQLFGIRYGAMVTKE
                     PLYDTRNEKIKS"
     gene            complement(3120..4025)
                     /locus_tag="VGT44_22420"
     CDS             complement(3120..4025)
                     /locus_tag="VGT44_22420"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HEV2583633.1"
                     /translation="MQTPTSVERIIDQVPGWSAAEAVVAPLEGGITNQNYRVEIHGET
                     FVLRIGGKGTHLLGIDRARESICTAIAARVGVGAEVMHFLAEEEALVTRFIEGSAITP
                     ESAAQAETLRRIVDAMQRYHDGPTFPGFFSPFETVRSYHRLALERGVPFPDTLPQVFA
                     LMARIEEALGHVQQQHPCHNDLLASNFIDDGHNIRILDWEYAGMGDRFFDLGNFAVNQ
                     ALDNERCNLLLSSYFGEARPEDIAHLHLMRLASDLRESFWGFLQMGASELDFDYKEYA
                     HHHLNRFLRNVEISPFEEWLTAVGH"
     gene            4324..5559
                     /locus_tag="VGT44_22425"
     CDS             4324..5559
                     /locus_tag="VGT44_22425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583634.1"
                     /translation="MIQKLLPKRRLTLWLVSCLAILALVPAIILFHPGMPAHASGGGG
                     GGGSPSASIYPTQGSPGTVVNINGFNYTPGTKVNIYFQTKANGVVNVVADPGGFFYAS
                     LTLPNTYTPGVRYYVHVNSVAFNESILFTFIKPNIQIFAGYNGTITFGATAQVSGGGF
                     LPNETVNLTWNIGSLGTQKAGVAVAGYDGSVFSNITMPSLPHGVQASLVATGLISGLT
                     ASTLVVESPAIYSTPTQSVVGTSVKVQGGGFGSNEHVQVLFQNKPVASPTTNAKGAFS
                     ATFVVPTTAQTGYQTNGIIATGKTTGVGAQAFFTVLPNVTISPNSGASGTYITVHGSH
                     FSPNDYVQILWVFPNNTGGSGGSGGGTIFIGSASVNSHGTFTATVQAPGGLVSGVKYF
                     VQVIDYQNGASNQAAFRSL"
     gene            complement(5620..5748)
                     /locus_tag="VGT44_22430"
     CDS             complement(5620..5748)
                     /locus_tag="VGT44_22430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583635.1"
                     /translation="MLGEVQELVRKHDYRYQLEARGDEMTSWRRAAQICAGQPGVR"
     gene            5951..6067
                     /gene="rrf"
                     /locus_tag="VGT44_22435"
     rRNA            5951..6067
                     /gene="rrf"
                     /locus_tag="VGT44_22435"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            6233..8542
                     /locus_tag="VGT44_22440"
     CDS             6233..8542
                     /locus_tag="VGT44_22440"
                     /inference="COORDINATES: protein motif:HMM:NF015476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009044 - xylan 1,4-beta-xylosidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 52 protein"
                     /protein_id="HEV2583636.1"
                     /translation="MRKWRLSGFLFLLGVVVISSLFFSLRSIDASAHASISGSPAAVP
                     AQLRLDSRLPAQTIASGANNVNAKFGTRFDFVVSPSLSAVYSGTDGVYHDNAASYIVG
                     VAPSGTGRTYIFHMGTPDSARGDTYLRNEQWTQGLDTTRWEGDAGTTSLHVTLDIIDS
                     FLGEPGCISIADCARQVQDDTVPVFLIGVTLHNGSIAAQTGDFLFGSNRALAPDNACA
                     QHVTSDGAQVNVLSCNPAADVTGGTLFLAGGQAQWQCNTSVSDRAGLAWHYNLQTGEN
                     QTAYMLIGGWNASQNLFLNTQLPTACQQEVLYSAREWSTRNAVVDFAIDNLATGDNLL
                     GRAQAMENTLINNDTLSPQQRWVIADALHSYKAASWLVGRTCAGANNFNGGYDAAVYE
                     GSFGFLTTVDVMHEYGYFEINRVPWFFKSALLTVFKNVQRDKFGIYFQHDQGGDVNSK
                     GLCTNPGKGIPTIRSTCYAPPRFSFGTPMPTEEDSNVALLTAYYIYVTGDSGLLTDTN
                     NAIMRLIDAGMQHNERVGDPATGIAYNNQDTTTTYDDQKDCLHNSTSNAGDLYYQGLK
                     EATAYRATAYLDSYVQGDTRGATWQRDAGKIEAAMVREYNVIGYIPLAETNDAYNNCA
                     GRTPVTGEGLFYLHLIGLDNTMNRTLLDDLAQEYPTDLKANILTSPLMISLESTNATG
                     SQCYLGGVCLRYEWFSKIMLSSLVADMIYTQYGCSSCARTDTSSAIFVHDTQLLLNFG
                     DGLRSNGSDWPGHYYPRGMISWAFLSAEY"
     gene            8573..>9395
                     /locus_tag="VGT44_22445"
     CDS             8573..>9395
                     /locus_tag="VGT44_22445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583637.1"
                     /translation="MQNLWDDDTSIDAAQHAVKRSAGRQPGRRAPTDAATLAALRAWL
                     LHDYIVPYCHSLAATRIFRRCFWIDGLGESRTTPSNLQDAGAAAQELAAQNRPMLLQT
                     LALQPGKSNRGRASATQDKKAKEQPEQAQETPFVLPKQNSLVNADWPTIAPALLASLD
                     QAAAIFLLNPLANSPSATSPFFAHRDLAPLYTRTAPTELCLLLSHAQIESRLLPALRT
                     PAGAAAFTALLRSDRWKAALAGSLIERPYHTITGHLRAAIRPHFLTAQPIAFPVCT"
BASE COUNT         1895 a         3019 c         2600 g         1881 t
ORIGIN      
        1 atccgaactg ctgggctggc cgatcaatcg gcggtgagtg cggtaaaccg gctcctacag
       61 aggtttggct ctattcctgc tcctggttct ccaccgctca gcaaatgctg cccatcgcga
      121 cactgaagtg ccggcgtgac accggcagcg ctttagcgcc tggcgcggca cacagtctgc
      181 aacccaaccc ccccgtcatc atgtagagcg cgccacggct ctcgcgcgcc aatgcgcagc
      241 cgttttcacc gcacaacctc cccacatccc tgacgctctc tacatgattg ccagccccac
      301 gtttcacaca cccccactgc ctcatccacc cttcagcagg cgctgcctgg caaacaacac
      361 ctgccacctg tgtttccaca ccggcacttc actatcgcga cggggtaaac tgcatttgct
      421 gcttcggaag aataccttac tctgctggag aaaccctcct caccacgtgt acccccacta
      481 ctcccatacg cattcactgc ggtgacgcgc gttcgtcgtc acatagttat tgtagagaat
      541 aaagaacacg ttcgcggtta cgaacgcatg gaggttgagt tgagaaacag gcgttcttca
      601 tgtgaaaatc ttaaccattt atcgaattat ttacgtattt ctatcgatat ttatatattt
      661 cgatgaatag gttttatgac ttaatcttct cattcctggt atcatacaac ggctcctttg
      721 tcaccattgc tccgtacctt atcccaaata attgcacatc aaccctcatt cccaccgctg
      781 catattctac cggcagatag ccgcaggcga tgctctggcg catggtgtag ccgtagcccg
      841 cgctggtgac gtggcccagc acacgctcgc catccaggaa aggctcacca cccagaccaa
      901 cggcggcggg atcgtccagc acgaggcagc acagtttgcg tttcacaccc tcggccttga
      961 ttcgttcgag cgcgtccctg ccgatgaaat cgcccttttg cagcttgacg gcgaagccca
     1021 ggcccgcctc gtagggattg tattcggaat ggatgtccgc gctccagtag cgatagccct
     1081 tttccaggcg cagcgattcg atggcgcgat agcctgccgc gacaattccc agcgcttgcc
     1141 cggcctgcca gagcgtgtcc cagagcttga ggccgtattc catcggggcg tagatctccc
     1201 accccaattc gccgacgtag gtgacgcgca gcgccagcgc cgggatatcg ccgagtgtga
     1261 cgtattgcgc ggtcaggtag ggaaaggccg cgttcgagaa atcatcttcg ctcaccgatt
     1321 gcagcaggtc gcgcgcgcgc ggcccccata cgccgaggca gcagcggctt gaggtgatgt
     1381 cgttcaggta gaccgagccg tcggtcgggg catggctgcg aatccaggtg aggtcgtgat
     1441 tgccgaaggc cgtgccggtc acgatctgga agcggtcggc agccaggcgt gtgacggtca
     1501 ggtcgcattc gatgccgccg cgcgggttgg gcatctgcgt ataggttatg ctgccgacgg
     1561 gctgatccat ctggttgtcg gtgatgcgct gcaagaaggc cagcgcgcct ggaccgagaa
     1621 tctcgatctt gctgaacgag gtctcgtcga acagggcgac ccgctcgcgc gtggcctggt
     1681 gttccgcgcc gatggcgggc gaccagtagc gcctggccca gccgtagggc gcgggccagc
     1741 cctggccttc cgcgagcgac tcgttgctgg cgaaccagtt cgggcgttcc caacccgcct
     1801 tttcgccgaa gaccgcctgg agctcttcca ggcggctgta gacagggctg aggcgcagtt
     1861 tgcgcgcgct gctgcgttcc tgcatggggt aagcgatgtc gtagtaggtg ctgtagacct
     1921 cgttgacgcg cgttgcgata tagcggcggc tggctgtata agcgccgaag cgccggatat
     1981 ccatgtgcca cagatccagg ctcggctctc catcgatgat ccattccgcc atcatcttgc
     2041 cgaccgcgcc ggagccggag acgccgtgcg cgcagaaacc gcaggccgcc cagaagcccc
     2101 tgacatccgg tgcctcgccc aggataaact cgccatcggg cgtgaacgat tccagcccgt
     2161 tgacacgcct ggtcagctcg acctcgcgca gcatgggaag acgctcgacg cagccgcgca
     2221 gcaggctctc gaaacgcggc gggtcgaagg gcaacacagt cggattatcg ccagcaggga
     2281 tgtcatcaac atcgagcggc agtgggtcgc gttcgtagcc acctaagacc aggctgtcgc
     2341 catcctgccg gaaatagacc aggttatcag ggtcgcgcaa gttcgggacg cgcaggccgc
     2401 cggacagttc gggcaagggc gcggtctggg cgtactggtg ctgcatgggg atcagcggaa
     2461 tggtgacgcc cgccatgcgg ccaatgcgcg gcgaccagat gcccgccgcg acgacaaccg
     2521 tctcggtgcg gatacgcccc cgtgaagtgt tgacggcctg cacgcgcccg ttgacgacct
     2581 caatgccggt gacgcgcgta tgcgggtgga acgtcacgcc gccttcgcgc gccctgtccg
     2641 ccagcgcctg agccaggatg ggcgctctgg cggagccgtc ggtcggcagc cacagcgcgc
     2701 cctcgacccc ttgcaggctc atcatgggaa acatggcttt cgcctcgctc gcgctaataa
     2761 gttccgcctc caatcccacg cttttcgaga aacccacctg ccgcttgagt tcctccatgc
     2821 gctccttcga cgaggcgaga cgcaggctgc tgccgcccgc gtagcccgct ttgccgtcca
     2881 cccccaacgt tttataaagc gagacgctgt agaccagcat cttcgtcaga ctcacggagg
     2941 agcgcagttg cccgaccaat cccggcgcgt gcgaggtcgt tccgccgatc agcggcccct
     3001 gttccagtac aacgacatcg cgccacccgc gttccgccag gtgataggcg atgctggaac
     3061 cggcgatccc actcccgatg atgacgactc tggcctggtc ttgcataaat ggatgctcct
     3121 taatggccaa ccgcagttag ccactcctca aatggcgata tctccacatt tcgcaaaaat
     3181 ctgttcaggt gatggtgtgc gtactcttta taatcgaaat ccagctccga cgcgcccatt
     3241 tgcaaaaagc cccagaacga ttcgcgcaga tcggaggcaa gtcgcatcag gtgcaggtgc
     3301 gcgatatcct ccgggcgcgc ctcgccgaaa taggacgaga gcagcaggtt gcagcgctca
     3361 ttgtccagcg cctggttgac ggcgaagttg cccaggtcga agaaacggtc gcccatgccc
     3421 gcgtactccc aatccaggat gcgaatgttg tggccatcat cgatgaaatt gctggcgagc
     3481 aggtcattat ggcagggatg ctgctgctgg acgtgcccca gcgcctcttc gatgcgggcc
     3541 atcagggcga atacttgcgg gagtgtatcg gggaatggaa cgccgcgttc caacgccagg
     3601 cgatggtagc tgcgcacggt ctcgaaggga gaaaagaaac cggggaaggt cggtccgtcg
     3661 tgatagcgtt gcatggcatc gacgatgcgc cgcagcgtct ccgcctgcgc ggcgctctcc
     3721 ggggtaatgg ccgagccttc gatgaagcgc gtcaccagcg cctcctcttc cgccaggaaa
     3781 tgcatgacct ccgcgcccac gcccacgcgg gcggcgatgg cggtgcagat gctctcacgc
     3841 gcccggtcga tgcccagcag gtgtgtgcct ttgccgccga tacgcagcac gaaggtctcg
     3901 ccatgtatct ctacgcgata gttctggttg gtgatgccac cctccaatgg agcaacgacc
     3961 gcctctgccg cgctccagcc tggaacctgg tcgatgatgc gctcaactga cgtgggtgtc
     4021 tgcatgattt ccctccctct tcatgagcat acctgctcga cagggcagcg tcaagcgcta
     4081 gtttgcccat aattcgcgca ggattccagc gaggaatagc tgtggttgag aaatcttgac
     4141 cagatgtgcg cagttcacac tacattagtg aactatctca cttgttccgt aggtgtttct
     4201 accgtatgat tgctagcggt gtgtgaagct caagcaaaat ttccccatga tacccggcaa
     4261 cgaagttggg tggaggattg aatctactaa agtgcctatg ctcgtatcaa aggaggattg
     4321 agcatgatcc agaaattgct tcctaaaagg cgtcttactc tctggcttgt ctcttgcctg
     4381 gctatcctcg cgctcgtacc tgcgatcatc ctgtttcatc caggtatgcc cgcgcacgct
     4441 agcggtggcg gtggtggcgg tggtagccct tcggccagta tctatcctac acaaggcagt
     4501 cccggcaccg ttgtaaacat caacgggttt aactatacgc cagggacaaa ggttaacatt
     4561 tactttcaaa cgaaggccaa cggtgtcgta aatgttgtgg ccgatcccgg cggttttttc
     4621 tacgcctcgc tcacgctgcc gaatacctac acaccgggcg tgcgctacta tgtgcatgtc
     4681 aatagcgtag cgttcaacga gtcgatcctg ttcacgttca tcaagcccaa catccagatc
     4741 ttcgctggat ataacggcac catcaccttc ggcgctacag cgcaggtcag cggcggcggc
     4801 ttcctaccaa acgagaccgt taatctcacc tggaacatcg gctcgctcgg cacgcagaaa
     4861 gctggcgtcg ccgttgccgg gtacgacggc agcgtcttca gcaatatcac catgccgagt
     4921 cttccccatg gcgttcaggc atccctggtc gccaccgggc ttattagcgg cctgaccgcc
     4981 tctaccctcg tggttgaatc cccggctatc tactctacac cgacacagag cgtcgtcgga
     5041 acgagtgtca aggtgcaagg cggcggcttt ggtagtaacg agcatgtgca ggtcctcttc
     5101 cagaataagc cggtagcgtc cccgacgacc aatgcgaaag gcgcattcag cgcgaccttt
     5161 gtcgtgccga cgacggcgca gacaggctac cagaccaacg gcatcatcgc caccggcaag
     5221 actacgggcg ttggcgcgca ggcgttcttc acggttttgc cgaatgtcac gatctcgcca
     5281 aattctggcg caagcggcac gtacatcact gtgcatggca gccacttctc gcctaacgac
     5341 tacgtgcaga tcctgtgggt cttccccaac aatacgggcg gctccggtgg ctcaggcggt
     5401 ggcaccatct ttatcggctc agcctccgtc aactcgcatg gcaccttcac cgcgaccgtc
     5461 caggctcccg gcggactggt cagcggagta aagtacttcg tgcaggtgat tgattaccaa
     5521 aacggcgcca gcaaccaggc agcattccgg tcgctctaaa cctcgctacc aatgcagtag
     5581 gcgccggtca aacggcgcct actgcattgg tccccaccct caccgcacac cgggttgccc
     5641 ggcgcaaatc tgcgcagccc tgcgccagga agtcatttcg tcgccgcgag cttcgagctg
     5701 gtagcggtag tcgtgcttgc gcaccagttc ttgcacctcg cccagcacgc ggcgcgcagg
     5761 gcatcgttgt ctcgcgagcg actggccggt gtttctttgt tcggcataac gcgctggcct
     5821 ttcaaatgct ggtaacgtat acgcaatcgc ggcaatgagc gccaggaatc gccagaaaaa
     5881 gtattgacaa tcccgtcatc ccgtagtatt atagctcttg tcaatacgat ccgcctgtgt
     5941 gcgcaggtga accggtgata atagcgaaga gggtacaccc gttcccatcc cgaacacgga
     6001 agttaagctc ttcagctccg atgatacttg aggggcagcc ctccgggaaa ataggacatc
     6061 gccgtttctc ccgctcaccc aggcggtttt gttcttccct ctcattttct gatgccagca
     6121 gttgtattcc tgttccagaa gtaggtcaac ccgccaaaag tcctctttca caggtctgat
     6181 cgtacgcaat tcgtttgtac acagtagatt gacacaagga agaggtagta ctgtgcgaaa
     6241 atggcgtctc tccggttttt tgttcctgct cggcgttgtg gttatttcca gcctcttttt
     6301 ttcactgcgc tccatcgatg catctgcaca tgcgagcata agcggcagcc ccgccgccgt
     6361 tcccgctcag ctgcgcctcg atagccgtct gcccgcgcag accattgcat ctggcgccaa
     6421 caacgtcaac gccaaatttg ggacgcgctt cgattttgtc gtctctccca gcctcagtgc
     6481 ggtctatagc ggcaccgacg gcgtctatca cgataacgca gccagttata tcgtcggagt
     6541 tgcgccgtcg ggaacgggca ggacctacat cttccatatg ggaacgccgg acagcgcacg
     6601 cggagatact tatctgcgca acgagcaatg gacgcagggc ctggatacga cgcgctggga
     6661 gggagatgcc ggtacgacca gcctgcacgt cacgctcgat atcatcgatt cgttcctcgg
     6721 cgaacccggc tgcatctcta tcgctgattg cgccaggcag gtacaggatg ataccgtgcc
     6781 ggtcttcctg atcggcgtga ccttgcataa tggcagcatc gcggcgcaaa ctggcgattt
     6841 tctcttcggc agcaaccgcg cgctggctcc cgacaacgcc tgcgcgcagc acgtcacctc
     6901 cgatggcgcg caggtcaacg tcctgtcctg caaccccgcc gccgacgtga ccggtgggac
     6961 gctcttcctg gctggcggcc aggcccagtg gcagtgcaat acaagcgtca gtgaccgcgc
     7021 cgggctggcc tggcactata acctccagac cggcgagaac cagaccgcct acatgcttat
     7081 cggcggctgg aatgccagcc agaatctctt cctcaacacc cagcttccca ccgcttgcca
     7141 gcaagaagtg ctgtattccg cgcgagaatg gtcaaccaga aatgccgtcg tcgatttcgc
     7201 catcgacaac ctcgcgacag gcgataacct tctcggacgc gcccaggcca tggagaatac
     7261 cctcatcaac aacgacacgc tctcgccgca acagcgctgg gtaatcgccg atgccctgca
     7321 cagctacaag gccgcgagct ggctggtcgg gcgcacctgc gcgggcgcga acaacttcaa
     7381 tggcggctac gacgcggcgg tctacgaagg ctcgttcggc ttcctgacaa cggtggatgt
     7441 catgcacgag tatggctatt tcgagatcaa ccgtgtcccc tggttcttca aaagcgcctt
     7501 gcttaccgtc ttcaaaaacg tgcagcgcga caaattcggc atctatttcc agcatgacca
     7561 gggcggcgat gtgaacagta agggtctctg caccaacccc ggcaaaggca tccccactat
     7621 tcgcagcacc tgctacgccc cgccgcgctt ctccttcggc acgccaatgc ctaccgagga
     7681 agactccaac gtggctttgc tgaccgccta ctacatctac gttaccggcg atagcgggct
     7741 gctgacggac actaataacg cgatcatgcg gctcatcgac gccggtatgc agcacaatga
     7801 gcgcgtgggt gaccccgcga ccggcattgc gtacaacaac caggacacga ccaccaccta
     7861 cgacgatcag aaagactgtc tgcacaactc cacgtccaat gccggcgatc tctactatca
     7921 aggcttaaaa gaggccaccg cttaccgagc tacagcctat ctcgacagct atgtgcaggg
     7981 ggacacgcga ggcgcgacct ggcagagaga tgccggcaaa atcgaggcgg ccatggtgcg
     8041 agagtataac gttatcggct acattcccct ggccgagaca aacgatgcct acaacaactg
     8101 cgccgggcgc actcccgtca ccggcgaagg actcttctac ctgcacctga tcggcctcga
     8161 taacaccatg aacaggaccc tgctggacga cctggcgcaa gaatacccaa ccgatctgaa
     8221 agccaatatc ctcacttcgc ctctcatgat ctcgctcgag tccaccaacg ccaccggctc
     8281 gcaatgctat cttggcggcg tgtgcttgcg ctatgaatgg ttcagcaaaa tcatgctctc
     8341 cagcctggta gccgatatga tctacacaca gtacggctgt tcatcctgcg cgcgcaccga
     8401 tacctcctcg gccatcttcg tccacgacac gcagctcctc ctgaatttcg gcgacggcct
     8461 gcgcagcaac ggcagcgact ggcccggcca ctactacccg cgtggcatga taagttgggc
     8521 attcctgagc gcggagtatt gaggcatgtg agtatggtaa aataatccca ctatgcagaa
     8581 cctgtgggat gacgatacca gcattgatgc cgcgcaacat gcagtaaaac gtagcgcggg
     8641 gcggcagcca gggagacgcg ctccaacgga cgcggcgaca ctggccgcgc tgcgcgcctg
     8701 gctgctgcat gactacattg ttccctattg ccattcgcta gccgccacgc gcatcttccg
     8761 ccgctgcttc tggatcgatg gactgggcga atcacgcact accccctcca accttcaaga
     8821 tgccggagca gccgcgcagg agttggccgc gcagaacaga ccgatgctgt tgcaaacgct
     8881 ggcgctccag cccggcaaaa gcaatcgcgg cagggcttcg gcaactcaag ataagaaggc
     8941 gaaggagcag ccagagcaag cccaagaaac gccgttcgtc ttgccgaaac agaacagcct
     9001 cgtcaacgcg gactggccga ctatcgcccc cgccctgctc gcatcactgg accaggctgc
     9061 cgccattttc ctgctcaatc cactcgccaa ctcgccctcg gcaacatccc ctttcttcgc
     9121 gcaccgggac cttgcgccgc tttacacgcg caccgctcca accgaactct gcctgctcct
     9181 atcacatgca cagatagagt cccggctgct gcccgcgctg cgcacaccgg caggcgcagc
     9241 cgctttcacc gcgctcctgc gcagcgatcg ctggaaggca gcgctggccg ggtccctgat
     9301 agagcgtccc taccacacta taaccggcca tttgcgggcg gccatccggc cccattttct
     9361 tacagcgcag cccattgctt tccctgtttg caccg
//
LOCUS       DASZAK010001277         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_672783, whole genome shotgun sequence.
ACCESSION   DASZAK010001277 DASZAK010000000
VERSION     DASZAK010001277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672783"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..1592
                     /locus_tag="VGT44_22450"
     CDS             129..1592
                     /locus_tag="VGT44_22450"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV2583638.1"
                     /translation="MNTPEGYITFRYMETTLPVLLSIWPILAGARLVRGEEERGTMDV
                     LLATSQSRIRLLLEKVGALVIALLVIAVLFALGLVGGEAALGGGHVDFVRALLAGLNL
                     GLIAFFFGMVALLISQLTASRRAAAGLASGLLILFVLLNIAGRELSGSWVQYLSPLYY
                     YNLNRPLLAGFPDQPLAALVPLGLSVLCLVGSVILFARRDIGRSAFESQRERAGGTQQ
                     ALRSLSRAEREVSTRSVSLQTLFADGWGSFWWLFGIVAFCAALQFVTPGFQKAFNQAV
                     QQTPWLAAYLSDTPTNTNAAMLGTLVFAFVPALVMILALTLALKWSSDLENGRLELIF
                     STPQSRRRVLLERFGANILIVLLAPVVTWLTITIGAQLANLVIDQGRILAASFSMLPP
                     ALIAMGLVYAASGRLRYGAVLGIVTAYLVLSYVQESLEGNIQFPGWLMSLSVFHLYGN
                     PAFLGMNWTNFLGMTGVGIALVVIGLVQFRSADIQLG"
     gene            1903..2703
                     /locus_tag="VGT44_22455"
     CDS             1903..2703
                     /locus_tag="VGT44_22455"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2583639.1"
                     /translation="METIDEIVQKWPLRRVGLRVQNMASELEYYTHFGLSIVRDGRNG
                     GDGSVGLGAGRREVLTLRELPGGRARPPRTAGLYHFALLVPSDEELGSFLQHLLDQRI
                     PLDGASDHLVSQALYLSDPEGNGIEVYADRPRAQWQAPEPGMGRIRMATNPLNAPALL
                     RLAGSYSGFSDGLRLGHMHLNVGDLDRSREFYGQLGMELMVGLPNSADFVSWDGYHHH
                     LGMNLWAGRNAAPEQPDMYGLDFFEIKRPGLAGGTLQDPNGVTVRVLE"
     gene            2910..>3908
                     /locus_tag="VGT44_22460"
     CDS             2910..>3908
                     /locus_tag="VGT44_22460"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEV2583640.1"
                     /translation="MGYRYAVIGSGRQGTAAAYDLARFGDADSIVVADMALEQAQRAA
                     ERVNRLAGRAVARAAAVNVRDEDAVVALLGGTGTQVFVSGVPYFFNMGLTRAALRARA
                     SMCDFGGNTGQVRQQLAFDAEAKAAGITLIPDCGQVPGLGTSLITYAMSLLDEPRDII
                     MYDGGIPRYPVPPWNYILTFNIEGLSNEYFGTTLFLRDGKQIEMACFEEYELVDFPEP
                     IGRLEAFTTAGGTSTMPWTFEGTLRTLQNKTLRWPGHYAQWKAFNDAGLIGLEPVEVD
                     GQRAIPRHLLHALLEPQILARPGDQDVVIVRIIARGLKDGREHEVVVDLFDYYDEAT"
BASE COUNT          674 a         1114 c         1255 g          865 t
ORIGIN      
        1 attcaccctg gacgttcaat tgcagggtgt gttcatccgc gccttgagcc acttttgcca
       61 caccggcggt ggtgacagcc tttatcatct tcgcgcgcat catcagcttt ctcagcgatc
      121 cctatgcgat gaataccccc gaggggtata tcacctttcg ctacatggaa accaccctac
      181 cggtcctact cagcatctgg cccatcctgg ctggcgcccg cctggtgcgc ggggaagagg
      241 aacgcggcac catggatgtc ctgctcgcta cgtcacagtc acgcattcgc ctgctgctag
      301 agaaggtggg cgcgctcgtg atcgcgctgc tcgtgatcgc ggtgttgttc gctctaggtc
      361 ttgtaggagg ggaagcggct cttggcggtg ggcacgttga ttttgtcaga gcgctgctgg
      421 cggggctgaa cctgggactg atcgccttct tctttggcat ggtagcgctg ctcatctccc
      481 aattgaccgc cagccgtcgc gcggcagcag gcctggcaag cggcctgctg atcctgttcg
      541 tgctcctgaa catagctgga cgggaactga gtggcagctg ggtgcagtac ctctcgccgc
      601 tctattatta caatctcaac cggccgctcc ttgccggttt ccctgaccag ccgctggcag
      661 cgctggttcc gttgggtctg tccgtactct gcctggtggg aagtgtgatc ctgttcgcca
      721 ggcgcgatat tggcaggtcg gcctttgagt cgcagcgcga gagggcagga ggcacacagc
      781 aggcgctgcg cagcctgagc cgggccgagc gcgaagtctc tacacgctcc gtgagcttgc
      841 agacgctctt cgcggatggg tggggttcct tctggtggct gttcggcatt gtggcctttt
      901 gtgccgcgtt gcagttcgtg acgcccggct tccagaaggc atttaaccag gcagtgcagc
      961 agaccccctg gctggccgct tacctgtctg acacccccac caacacgaat gccgcgatgc
     1021 tgggtaccct cgtcttcgcg ttcgtgccgg cgctggtcat gattctggcg ctgacgctcg
     1081 ccttgaagtg gtcttcggac ctggaaaacg ggcgactgga gctgattttc agcaccccgc
     1141 agtcgcggcg acgggtcctg ctggaacgct tcggggcgaa tatcctgata gtcctgctcg
     1201 ctcccgtcgt gacctggctc acgatcacca tcggggcgca gctcgccaat cttgtcatcg
     1261 accagggcag gatacttgcc gccagcttca gtatgctccc tccggcgttg attgcgatgg
     1321 gcctggtcta tgcggcttcc ggacgcctgc gttatggcgc agtgctgggc atcgtgactg
     1381 cgtatctcgt gctatcctac gtgcaggagt cgctcgaagg gaatatccag tttcctggct
     1441 ggctgatgtc gctctccgtg ttccacctgt acggcaaccc ggctttcctc ggcatgaact
     1501 ggactaactt cctgggcatg accggcgtgg gcatcgcgct ggtggtgatt ggcctggtac
     1561 agttccgctc tgccgatatc caactcggct aaaaccactc gatattccgt ctcgcgttgg
     1621 cgatatcgcc ccgtgccttg tctgtggtga atcgtcagcc gagagcaata ttgttgccag
     1681 gatttcgcca gccccagatg cttcgctgcg ttcagcatga cagcccccgg ttgtcaccct
     1741 gagcgcggcg aagggtctcg gtcgatgggc ttagagatgc ttcgctgcgc tcagcatgac
     1801 agtggcagtt tcctctgcct cgtacttgca attctgaacg atatatggca taatatcagt
     1861 aagttactaa gtataagtaa gtattcgata ggagtgcatc caatggaaac aatagatgag
     1921 atcgttcaga aatggccttt gcgccgcgtc gggctgcgcg tacagaatat ggcgagcgaa
     1981 ctggagtatt acacgcattt cggtctgtcg atcgtgcgtg atgggcgcaa tggcggcgat
     2041 ggaagcgttg gactgggcgc tggtaggcgc gaggtgctga cgctgcgtga gttgccgggc
     2101 gggagagcga ggccgccgcg cacggctggc ctgtatcatt ttgctctgct ggttcccagc
     2161 gacgaggagc ttggttcgtt cctgcagcac ctgctcgatc agcgcatccc gctggatggg
     2221 gcgtcggatc acctggtgag ccaggcgctc tacctgagcg atcctgaggg caacggcatc
     2281 gaggtctatg ctgatcgccc gcgcgcgcag tggcaagcgc cggagcctgg aatgggccgc
     2341 atcaggatgg cgaccaaccc gctcaacgcg cccgcgctgt tgcgactggc gggttcttac
     2401 agcggcttct cagatgggct gcgcctcggc catatgcacc tgaatgtcgg cgacctggat
     2461 cgcagccggg aattttacgg gcagctgggc atggagttga tggtcggcct accgaacagc
     2521 gcggatttcg tctcctggga cggctaccac catcacctgg gcatgaacct gtgggccggg
     2581 cgtaacgccg ccccggaaca gccggacatg tacgggttgg atttcttcga gatcaagcgg
     2641 ccaggattgg ccggcgggac gctgcaagat cccaatggag tgacggtgcg cgtgttggaa
     2701 tagggactgg gcactgcgtg tcatgctgag cgcagcgaaa catctagttc gatgagctga
     2761 gatgcttcgc tgcgctcagc atgaccttgg gagatagctt tggtgaacga ctatggggtg
     2821 cgtacctttg cggtcctctt tcaaccttat cctattgata tggtagactc tatctatgga
     2881 tagagtctac catatcgtag gagacacaca tgggctatcg atacgcggtg atcggctccg
     2941 ggcggcaggg gacagcggcg gcgtatgacc tggcgcgttt tggggatgcg gattctattg
     3001 ttgtggcgga tatggcgctg gagcaggcgc agcgagcggc ggagcgcgtc aaccggctgg
     3061 ccgggcgggc ggtggcgcgc gcagctgctg tgaatgtgcg ggacgaggac gcggtcgtcg
     3121 cgctgctggg cggcacaggc acgcaggtct ttgtgtcggg cgtgccgtat ttcttcaata
     3181 tgggcctgac gcgggcagct ctgcgggcgc gggccagtat gtgcgacttt ggcggcaaca
     3241 ccgggcaggt tcgccagcag cttgctttcg acgctgaagc gaaggcggcg ggcattacgc
     3301 ttatccccga ttgcgggcag gtgcccggcc tggggacctc gctcattaca tacgctatgt
     3361 cgctacttga tgaaccgcgc gacattatta tgtatgatgg gggtatcccg cgctatccag
     3421 tgccgccctg gaactatatt ttgactttca atatcgaggg actctcgaac gaatatttcg
     3481 gcacgacgct tttcctgcgc gacggcaaac agatcgagat ggcctgtttc gaggaatacg
     3541 agctggtgga tttccccgaa ccgattggtc ggctcgaggc cttcacgacg gcgggcggca
     3601 ccagcacgat gccctggacg ttcgagggaa cattgcgcac gctgcaaaac aagacgctgc
     3661 gctggcccgg ccattacgcg cagtggaaag ccttcaacga cgctggactg atcggcctgg
     3721 agccggtcga agtcgatggg cagcgtgcca tcccgcgaca cctgctccac gccctgctgg
     3781 agccgcagat tctggcgcgt cccggcgacc aggatgtggt gatcgtgcgt atcatagcgc
     3841 gcggcctcaa agatgggcga gaacacgagg tagtggtaga cctgttcgat tactacgacg
     3901 aggctacc
//
LOCUS       DASZAK010001278         2346 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_423667, whole genome shotgun sequence.
ACCESSION   DASZAK010001278 DASZAK010000000
VERSION     DASZAK010001278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423667"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1690)
                     /locus_tag="VGT44_22465"
     CDS             complement(38..1690)
                     /locus_tag="VGT44_22465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HEV2583641.1"
                     /translation="MIDLEAPIKEVTVYSDRALVTRRGGIQLEAGEHELRVNNLPQFL
                     RDSLRAAGRGTKGIRILNVDVSTAFLSRPPEAELVSLQDELERLVQKQQLLEARRNAL
                     NDRRQWLRALGEQSRDFARGLAQGQMKPQDCADFFSFAANQALQDAEAAQGLELEMKQ
                     VQQEIAAKKRELAQKQGDTLADRLAAVVTVALADAGEFELELSYLVLGAAWHPQYDVR
                     VQMSGEQSEGDVELTYVGVVQQSTGERWENVGLSLSTARPSLAAVLPELDPLYLNVYT
                     PPIPRPMNVPAVPMRAARARIASTAAGNQQPMAMADMLASGEAAFEEEQEEQLAFMPA
                     DVATAAVEQTGTALIFRVGRSVDIPSDGSPHKTTIARDNLPCTFDYVSAPLIDEQVHL
                     RGAITNTSERVLLNGEANIFLSGEYVGATKVKTTAPAEKFKIFLGIDDSIKVKRELIE
                     RSVEKGNALQANMRRITYGYRITVHNYAAATRQVVIRDRLPVSQHERVKVKVQSIQPA
                     PTERTKLELVTWKFPLAAGAEQKIEYRFVVEYPQDLRVIGLP"
     gene            complement(1812..>2346)
                     /locus_tag="VGT44_22470"
     CDS             complement(1812..>2346)
                     /locus_tag="VGT44_22470"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEV2583642.1"
                     /translation="DRRVGFMHGLLNGSILLMNIVSLVLRLTGKRRAGVALSTTGYLA
                     SLFSAYLGGELAFAKGIGINHEAWEGGSDDFVAVMDAQELAEGKLTRVDVAGIPAVLL
                     KQGNSIYAIGAVCTHLAGPLDEGTCENGIVTCPWHGSRFHMSDGSVVNGPAVYAEPTF
                     AVRVRDGKIELRRLDHA"
BASE COUNT          531 a          745 c          557 g          513 t
ORIGIN      
        1 gatgttgacg agtgatcacc atctgattac atcgaattta cggtaaccct atcaccctca
       61 aatcctgcgg gtactccacc acaaagcgat attcaatttt ctgctcagca cctgcggcca
      121 gcgggaattt ccaggtcacc aactccagtt tggtgcgttc ggtaggtgcc ggctgaatgc
      181 tctgcacttt caccttcaca cgctcgtgct gggaaacggg caggcgatcg cgaatgacta
      241 cctgcctggt ggcggctgca tagttgtgca ccgtgatacg atacccatag gtaatgcgcc
      301 gcatattggc ctgtaatgca ttgcccttct ccacactgcg ctcaatcaac tcgcgcttga
      361 ccttgatgct atcatcgata cccaggaata tcttaaactt ttctgccggg gcagtcgtct
      421 tcaccttcgt cgcccctaca tactctccac ttaagaaaat attagcttca ccattcaaca
      481 gcacacgctc gctagtattc gtgatggccc ctcgcaggtg cacctgctca tcgatcaggg
      541 gagcactcac ataatcgaac gtgcacggga gattgtcccg cgcaatcgtc gtcttatgcg
      601 gagaaccgtc ggacgggata tccacagaac ggccaacgcg gaaaatcagt gcagtccccg
      661 tttgctcgac cgcagccgta gcgacatctg ccggcataaa tgctaattgc tcttcctgct
      721 cctcttcaaa cgcagcttcg ccggaggcca gcatatctgc cattgccatt ggctgttgat
      781 ttcctgctgc cgtcgaagcg attctggccc tggcagcacg cattggtact gcagggacat
      841 tcattggcct gggaataggg ggagtataga cattgagata caagggatcg agttcgggca
      901 acactgccgc caggctggga cgtgccgtgg aaagcgagag gcctacgttc tcccagcgct
      961 caccagtaga ttgctgcacc acgcccacgt aagtcagctc gacatcgcct tcgctttgct
     1021 cgcccgacat ctgcacccgt acatcatact gtggatgcca ggcagcgccc agcaccaggt
     1081 aggataattc caactcaaac tcgcccgcat cggccagcgc caccgtgaca accgcagcca
     1141 acctgtcagc aagtgtatca ccttgtttct gtgcaagctc acgctttttc gccgcgatct
     1201 cctgctgcac ctgtttcatt tccagttcca ggccttgagc cgcttcggca tcttgcaggg
     1261 cttgattggc cgcaaagcta aagaaatctg cgcaatcctg cggtttcatc tgcccctgcg
     1321 ccagtcccct ggcaaaatct cgcgactgct cgcccagcgc gcgcagccac tggcggcgat
     1381 cattaagcgc gttccggcgc gcctcgagta actgctgctt ctgcaccagc cgctccagct
     1441 cgtcctgcag gctcaccagt tcggcctccg gcgggcgact cagaaatgca gtgctcacat
     1501 cgacattgag gatgcgtata cccttcgtcc cacgtccagc cgcacgcagc gaatcgcgca
     1561 agaactgcgg cagattattg acacgtaatt catgctcacc cgcctcgagt tggataccgc
     1621 cacgtcgcgt aaccagcgcc cggtcgctat aaacagtcac ttccttgata ggtgcttcta
     1681 gatcgatcaa gatttacctc tcattcttta atagccgtaa aagttatttt gcacagcaga
     1741 agtcctaaaa atagggcgca actcactgcg ccccctacaa acaaatatgc tcgttagcca
     1801 tcgaaactat actacgcgtg atctaaccgc cgcaactcaa ttttcccatc gcgcacgcgc
     1861 acggcgaacg ttggctcggc atagaccgcc gggccattga ccacgctgcc gtcactcata
     1921 tggaatctcg atccgtgcca gggacacgtc acgatcccat tttcacacgt cccttcatcc
     1981 agcgggccag ccaggtgggt acaaacagcg ccaatggcgt aaattgaatt gccctgcttc
     2041 agcagcaccg ccgggatacc agccacgtca acgcgggtga gcttaccctc agccagctct
     2101 tgcgcgtcca tgacagccac aaaatcgtcc gaaccgccct cccacgcctc atggtttata
     2161 ccaatgccct tagcaaaggc aagttcacca cccaaataag cggagaaaag cgacgccagg
     2221 tagccagtcg tcgaaagagc tacacctgca cggcgtttgc ctgtaaggcg cagcaccaac
     2281 gagacgatat tcatcagtag tatactgcca ttaagcagtc catgcatgaa tccaacgcgg
     2341 cgatct
//
LOCUS       DASZAK010001279         3041 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_245139, whole genome shotgun sequence.
ACCESSION   DASZAK010001279 DASZAK010000000
VERSION     DASZAK010001279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245139"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..2499)
                     /locus_tag="VGT44_22475"
     CDS             complement(61..2499)
                     /locus_tag="VGT44_22475"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEV2583643.1"
                     /translation="MRSGLIESRPAGEQERSYPPVYAPSAVQQARVELQPTRAKTRGA
                     RGQARSTTIRPVQGWMAFVFLTIAVYVVVISFIQAGWVDGSVVLLYSTAAGLVVGLGV
                     AKVTRFPQSLLHLSACLAGYWLSVWLASEVALHIQWQSLLVDLRALIGGSLPSTLQVN
                     NQAIFLFYLTFLSFFLGYFGAWLIYRARLPWLVGVVYSSIMLVNLQAAHSDLSMTLVA
                     LMAALLLLIGQMQLNNQLARWTEEGLHTDRGWMRNITGRFMRITSLFMAAIILLCLVL
                     PVFDQPPAGVTFWYYLDNAWNRVLSNPSAIFNPASLLQPGSGSGAAPNFFGDQLPIAG
                     SVNLPSGEVLYYTTTGAGSQQGRYLESVAYDQFDGHTWTTSTSNGASQQYNPGATLPV
                     ESQSGVSQISTSVTIAKPPQGSKAYIFAPLQPKTFSVSMTLYGSGIISAWAQQSSLTA
                     GEKYQVISLMPVATEQQLSATPLLHSPIAAWQGDPNFNLLKNFYLEEPPDLSQTVITT
                     AHAWTQGATNVYEAMNMLVAHLSDQTRFTYSIDNQAVPGNIDAVTWLLQSRRGFCTYY
                     ATAMAMMARVLGVPARVVSGFNQGYYDAKRHEWVVIGEDAHSWTQVYFPNMGWINFDP
                     TPGFSLNPTAASQPTPTLTATPTRPGQTVTPTGTKHLTPTTGPGGGRSGGDPAPQAFG
                     GSQMLFLSFSLVVLVIAMLALAIAAYRYRENKRLEEGTLIAVTFWRLSRLAALAGHSP
                     GKSQTPYEYTYQLARRFPQARDALWRITHLFVRERWGAPQHAPAPGEEQTVEQLWPRL
                     RAIMLRALIFRQKP"
     gene            2794..>3041
                     /locus_tag="VGT44_22480"
     CDS             2794..>3041
                     /locus_tag="VGT44_22480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV2583644.1"
                     /translation="MALSFVLADLALSTIVWWLIVGLIAGFLASLVMRGGGYGIVGDI
                     IAGLVGAFIGGWLFSLLGISAGGGLIGEIIVAFVGALIL"
BASE COUNT          638 a          879 c          919 g          605 t
ORIGIN      
        1 ggcggtgtgc gcggtaaacc ggcacctacg gagatgcagc attccttcgt caatgcgcat
       61 ttatggcttt tgccggaaga tcagcgcgcg cagcatgatg gcgcgcagtc ggggccagag
      121 ctgctcgacg gtttgctctt ctccgggcgc gggcgcgtgc tggggcgcgc cccagcgttc
      181 gcgcacaaag aggtgggtga tccgccacag cgcgtctctg gcctgcggga agcggcgggc
      241 aagctgatat gtgtactcat acggcgtctg cgatttgccc ggcgagtgac cggccagggc
      301 agccaggcgc gagagtcgcc agaaggttac ggcgatcaag gtgccttctt ccaggcgttt
      361 gttttcacga tagcgatacg ccgcgattgc cagggccagc atagcgatca ccagcacgac
      421 cagcgagaac gacaggaaca gcatttgcga tccgccgaac gcctgcggcg ctggatcgcc
      481 acctgatctc ccgcctccgg ggccggtcgt gggcgtgaga tgtttcgtac cggtcggcgt
      541 gaccgtctgc cccggcctgg taggcgtggc agtcagcgtg ggagtcggct gcgacgcggc
      601 agtgggattc aacgagaagc cgggcgtcgg atcgaagtta atccagccca tattgggaaa
      661 atagacctgc gtccagctat gcgcgtcctc gccaatcacc acccattcat gacgtttcgc
      721 gtcgtaatag ccctggttga agccgctgac cacgcgcgcc gggacgccga gcacgcgcgc
      781 catcatagcc atcgctgtag cgtagtaggt gcagaacccg cgtcgcgact gtaacaacca
      841 ggtgacggca tcgatgttgc ccggcaccgc ctggttgtct atcgagtagg taaacctggt
      901 ctgatcgctc aggtgcgcca ccagcatgtt catggcctcg tagacgttcg tcgcgccctg
      961 cgtccaggca tgggctgtcg ttatcaccgt ctgcgacaga tcaggtggct cttccaggta
     1021 gaagtttttg agcaagttaa agttcggatc gccctgccag gcagcgatgg gcgaatgcag
     1081 cagcggcgtc gccgacaact gctgctcggt agcaactggc atcagggaga tgacctggta
     1141 tttctcaccg gcggtcaagg atgattgctg cgcccaggcc gaaatgatgc cgctgccata
     1201 cagggtcata gagacggaga aagtcttagg ctggagagga gcgaagatat atgctttgga
     1261 gccttgcggc ggcttcgcaa tcgtgacgga tgttgagatc tggctcaccc cggattgcga
     1321 ttcgactggt aaagtcgcgc cgggattata ttgctggctc gcaccattcg atgtggaagt
     1381 agtccacgta tggccgtcaa actgatcgta ggctacgctc tccaggtagc gtccctgctg
     1441 gcttcccgcg cccgtcgtcg tataatacag cacttcgccg ctgggcaggt tgacgctgcc
     1501 ggcgataggg agctggtcgc caaagaaatt cggcgccgcc ccggagccac ttcccggctg
     1561 taaaagcgag gcgggattga agatcgccga tgggttgctc aggacacggt tccaggcgtt
     1621 gtccaggtag taccagaagg tgacgcccgc agggggctgg tcgaagaccg gcagaaccag
     1681 gcacaacaaa atgatcgcgg ccataaacag cgacgtgata cgcatgaaac gcccggttat
     1741 gttgcgcatc cagccgcggt cggtatgcag cccttcctcc gtccaacgcg ccagctggtt
     1801 gttcaattgc atctgcccga tcaacaacag cagcgctgcc atcagcgcca ccagcgtcat
     1861 cgaaagatcg ctgtgggctg cttgcaagtt caccagcatg atcgagctgt agaccacccc
     1921 gaccagccag ggcagccgcg cgcgatagat caaccacgcg ccgaaatagc ccagaaagaa
     1981 cgagaggaac gtcaggtaaa agaggaagat ggcctggttg ttcacttgca gcgtcgaagg
     2041 cagcgaaccg ccaatcagcg ctcgcagatc gaccagcagc gattgccatt ggatatgcag
     2101 cgccacttcg ctggctagcc agaccgagag ccagtatccc gccaggcacg cgctgagatg
     2161 cagcagagac tgcgggaagc gcgttacttt cgccacgcca agtccgacca ccagcccggc
     2221 tgccgtgctg tacagcagca ccacgctgcc atcgacccag cccgcctgga taaaagaaat
     2281 caccaccacg taaaccgcga tggtcaggaa cacaaaggcc atccacccct gcacggggcg
     2341 aatagtggta gagcgagcct gtccccgcgc gccgcgcgtt ttcgccctgg ttggttgcaa
     2401 ctcgacacgc gcctgttgta ccgctgatgg cgcatataca gggggataag accgctcctg
     2461 ttcccccgca gggcgcgact caatcaaacc agaccgcatc tacaggctcc tgtatctagt
     2521 ctcaccatag tctgcatgtt tatctatacg ctaaagtata ccagattgtc gcatcacagg
     2581 ccaaaaacac ggcgtttttg gctattaaac ctgatgaact tttccaaaca taacgggtca
     2641 tcgtgtctgt aggggtttct gacacaggca tcgagtttat caacgtttga tgcgctgcat
     2701 acgtgtatag catatatagc ttcacaaacg cctttgttca gagaggcatt tgcgaagatc
     2761 aagagtaaag gccaaagaaa aggagtatga tatatggcac tcagttttgt tttagcggat
     2821 ctcgcgctat caaccatcgt ctggtggtta atcgtcggat tgatcgcggg cttcctggcc
     2881 agtctcgtca tgcggggcgg cggctatggt attgtcggcg atatcatcgc gggcctggtc
     2941 ggcgcattca tcggcggctg gctgttcagc ctgcttggca tcagcgcggg cggtggcctg
     3001 atcggtgaaa tcatcgtcgc gttcgtcggc gcactgatcc t
//
LOCUS       DASZAK010001280         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_351590, whole genome shotgun sequence.
ACCESSION   DASZAK010001280 DASZAK010000000
VERSION     DASZAK010001280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351590"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1638)
                     /locus_tag="VGT44_22485"
     CDS             complement(<1..1638)
                     /locus_tag="VGT44_22485"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: protein motif:HMM:NF008559.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HEV2583645.1"
                     /translation="MAEIMNTSASGRAILHPLEPLTAGEIAEVTRVVRMNEGLSPHFR
                     FISVTLHEPPKELVRTFTEGDSFTREAEVILIDQGKTYEMVVDIVGGRVVEFQHVPGI
                     QAPIPFEEAIECETCVKSYPPFQEAMRTRGITDMDLVMVDSWPAGYYGADDDASRRLS
                     RPLIFVRFGPNDNGYAHPVEGLHLVVNLTNMEVVLFEDHGVMPIPPEAGNYTPDTTGS
                     LRSDLKALEITQPDGPSFLVKDGYLVRWQKWQFRIGFTHREGLVLYNVGYEDQGRLRP
                     ILYRMSVAEMIVPYGDPSALHYRRNVLDMGEHGLGVQANSLELGCDCLGEIAYFDAHL
                     TDGQGNVVTKKNVICMHEEDYGVLWKHTNFRTEEVQVRRSRRLVVSFFTTVGVYDYGF
                     FWYFYQDGSIQVEVKLTGIMSTRGMLTGEPAKPKYGTLVAPGLNAMIHQHFFSFRMDM
                     EVDGPQNSVSEVHIEAEPEGPMNPYGNAFFAKETLFTREQEAQQVIDPLAGRYWKVIN
                     SHSRNRMGDAVGYKLMPDENAVAFAQPGAFFLKRALFT"
     gene            complement(1686..>2443)
                     /locus_tag="VGT44_22490"
     CDS             complement(1686..>2443)
                     /locus_tag="VGT44_22490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583646.1"
                     /translation="VAYFVTTWALLVVEGQNAAFSTGNPVQLVFGTVGSGLGKFAVDV
                     TAVCVMLFFIVVAIIYNVIFARLLMTAGIDQRLPLIVTRLNRNRVPVNAVLIQTGISV
                     VVTLVIFFVLPSFTFLGNPAELTNKAYLTTAAALLLIWAFSFLFPFIDLFIIIVREPR
                     KVFARSRIFPTPVLVVSIIAGIFVCLATIVDTLLFSFAPALIPNGTWSLMVGIYSLIC
                     LVLCAVAGMVATGEADVEQWKFDETLLKDVSKQ"
BASE COUNT          618 a          677 c          634 g          514 t
ORIGIN      
        1 ggtgaagagc gcccgcttga ggaagaaggc acccggttgc gcgaaggcca cagcattctc
       61 atccggcatc agcttgtagc ctaccgcatc gcccatgcga ttgcgtgagt gggagttgat
      121 gaccttccaa tagcgaccag cgagaggatc gatgacctgt tgcgcctcct gctccctggt
      181 aaatagagtc tccttagcga agaaagcgtt gccatagggg ttcattgggc cctcaggctc
      241 tgcctcgatg tgtacttcag agactgagtt ttgaggtcca tccacctcca tatccatgcg
      301 aaagctgaag aagtgctggt ggatcattgc attcagccct ggtgcaacga gcgtcccata
      361 cttcggtttg gccggttcgc ctgtaagcat gccccgcgtg ctcataatcc cagtgagttt
      421 cacctctacc tggattgaac catcctggta gaagtaccag aagaacccgt agtcgtagac
      481 gcccaccgta gtgaagaagg atacgaccag acgtcgcgag cggcgcacct gtacctcctc
      541 tgtgcgaaag ttggtatgct tccacaacac accataatcc tcttcgtgca tacagattac
      601 gttctttttc gtgacgacat tgccttgacc atctgtgagg tgcgcatcaa aataggcgat
      661 ctctccaagg cagtcgcacc ctagctccaa actattagct tgcacgccaa ggccgtgctc
      721 ccccatatcg agaacattgc gccggtagtg tagcgctgag gggtcgccat acggcacaat
      781 catctcagca acagacatgc ggtagaggat cggacgaaga cggccctgat cctcatagcc
      841 aacgttatac agcaccagtc cttcgcgatg ggtaaagcca atgcggaact gccacttctg
      901 ccacctgaca aggtacccgt ccttcaccag gaaactgggc ccatctggtt gggtgatctc
      961 cagcgcttta aggtcactcc gtagcgaacc tgttgtatct ggcgtgtaat tgcccgcctc
     1021 cggcgggatc ggcatcacgc cgtgatcctc aaagagcacc acttccatgt tggtgaggtt
     1081 aaccacgagg tgcaaaccct ccacaggatg ggcatatcca ttatcgtttg gaccgaagcg
     1141 gacaaagatc agaggccgcg ataggcggcg cgatgcatcg tcatctgcgc cgtaataccc
     1201 ggcaggccag gaatcaacca tcaccaaatc catatcggtg atccctcgtg tgcgcatagc
     1261 ttcctggaaa ggcgggtagg acttcacgca tgtctcgcac tcaatggcct cttcgaaagg
     1321 gattggagcc tgaatgcctg gtacgtgttg gaattcgacg acgcgccctc ccacgatatc
     1381 aacaaccatc tcgtaggtct tcccctggtc gatcagaatg acctccgcct cccgcgtaaa
     1441 cgagtcgcct tcagtgaacg ttcgcaccag ttctttaggt ggttcgtgga gagtgacgga
     1501 aataaagcga aagtggggcg aaagtccctc attcatcctg acgacgcggg tgacctcggc
     1561 gatctcaccc gccgtcagtg gctccagtgg atgcaaaata gcacgacctg acgcagacgt
     1621 gttcatgatt tcagccatga aaaataatcc ttatctcttc tagtcagaat caacgaatga
     1681 cataactact gcttggatac atccttcaat aacgtctcgt cgaatttcca ttgctcaaca
     1741 tcggcctcgc cagtagccac catcccagca acggcacata aaaccaggca gatcagtgag
     1801 tagataccta ccattaacga ccacgtgcca tttggaataa gcgcgggcgc gaaggaaaat
     1861 agtagtgtat ccacgatcgt tgccaggcag acaaaaatcc cagcgatgat gctaaccacc
     1921 aggacgggtg ttgggaagat gcgggagcgt gcaaacactt tcctgggctc acgcactatg
     1981 atgatgaaca ggtcgatgaa tgggaagaga aaggaaaacg cccagatgag taggagcgcg
     2041 gcagccgttg tgagataggc cttattggtc agctccgccg gattccccag gaaggtaaag
     2101 gatggtaaca caaagaagat caccagggtc accaccacag aaatgcctgt ttgaatcaag
     2161 accgcgttga ccggaacgcg attccgattg agccgcgtta cgattagtgg caggcgctga
     2221 tcaataccag cggtcatcag caggcgggca aagattacat tataaatgat cgctactacg
     2281 atgaaaaaca gcataacaca cacggcggta acgtcgacag cgaattttcc cagtcccgaa
     2341 ccaacggtgc cgaagaccag ttgcacggga ttacctgtgc tgaacgcagc gttctggcct
     2401 tctaccacca ggagtgccca ggtggtgaca aaatatgcta cga
//
LOCUS       DASZAK010001281         5927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_208541, whole genome shotgun sequence.
ACCESSION   DASZAK010001281 DASZAK010000000
VERSION     DASZAK010001281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5927
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208541"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VGT44_22495"
     CDS             complement(<1..576)
                     /locus_tag="VGT44_22495"
                     /inference="COORDINATES: protein motif:HMM:NF040366.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrzE family protein"
                     /protein_id="HEV2583647.1"
                     /translation="MQNAATQSELRTLHSTGDLPPIQSEPPEPRGLESTEHDQSTETL
                     QGQTPVAGTPSPIQPSGPRAQQHALLPQRGLYYAAIAGVIAGVITALLTLVVTLVFAG
                     TFHAASQQIAVDKLTVKTALALAGLELLTVTLSLLIGFVVGLIVGRIAVRRRLGFLAG
                     ALAGAVFYLITFLVGLIPSYPGNLAVNGTTMT"
     gene            complement(730..972)
                     /locus_tag="VGT44_22500"
     CDS             complement(730..972)
                     /locus_tag="VGT44_22500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583648.1"
                     /translation="MFTLQFILADGVTIKLGDNVWTFGLNFIIYLIVAAVVGLVAEAL
                     VGRRLPFGFVGAIVAALVGAWLLSQVIIINGIGGVV"
     gene            complement(1045..1368)
                     /locus_tag="VGT44_22505"
     CDS             complement(1045..1368)
                     /locus_tag="VGT44_22505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV2583649.1"
                     /translation="MTLATLLLADINLSLSNVLWWLVVGLIAGFLASRVMRGGGYGLI
                     GDIVVGLIGAFIGGWLASFLGIGGSSSLIVTIIIAFIGACILLAILHAVSRGGTSRSR
                     GWGRR"
     gene            complement(1629..2675)
                     /locus_tag="VGT44_22510"
     CDS             complement(1629..2675)
                     /locus_tag="VGT44_22510"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEV2583650.1"
                     /translation="MGHLDNPTPLPIARSSSDYAELKRLIKEAGLLEKHPVSYAPKMA
                     LLVAMLALSVLFLLFVHSFWLQLINAAYLAIVTTQLGLLGHDAGHRQVFHTTWKNNLV
                     GLIVGNLLIGMSNAWWMDKHNKHHSHPNQRDLDPDINIPIIAFGEDDLHRRGWPFQAL
                     IKYQAYFFFPVLLLVAFDLQINGIQFLFREKVKYAALEWLLLGLHFACYFGLLFSHLA
                     AWQAILFILVHQGLSGFYLGSIFAPNHKGMPVLDADSQMDFLHRQVITARNVRGHPFT
                     DFWYGGLNYQIEHHLFPGMPRNQLKKAQGIIRAFCLAHSIPYYETNWWRSYGEIVQAL
                     HLVSAPLRQAKAVS"
     gene            3134..4522
                     /locus_tag="VGT44_22515"
     CDS             3134..4522
                     /locus_tag="VGT44_22515"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583651.1"
                     /translation="MKTEQRDENPSTASAVKDKPVASFRRVLKNRSFLLLWLAQLLSQ
                     IVFNAANYGVIAIVTAITHSTVMVGLAIVSFTLPAVPFSLLAGVYVDHLNKRLVLWVS
                     NALRAVATGLIVLALLWDPQTVIPLYILTFLISLVTQFFTPAEASAIPLLVNKDDLAP
                     ALSLFSITLTIAQALGFLLLGGLITVLFPPFQLSLGFIRVQVLSFDMLFVIVAVVYLF
                     STLLILAIPARALQQKQYEEPELLTSLGKQTWTVIQHDVREAWAYIRLDRALLLALLR
                     VSFVSILLLVIGELAGPFVVNVLYLPVQTMPLILAPAGLGLVLGGLFMPTLMRWLGKS
                     RTIAVGSLATAAGLILIPLGRLLWSRLALPVPGILFYVGAVTFCLGIALDMVNIPAQT
                     MMQEQAIEEERGRMFSFQSMLYNAGSIPVLLFAGVIADTLGVETVMYVVAAAILGFSW
                     WAARYSHRIFIS"
     gene            complement(4554..5219)
                     /locus_tag="VGT44_22520"
     CDS             complement(4554..5219)
                     /locus_tag="VGT44_22520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583652.1"
                     /translation="MLLRSLIQLGGVYRAIQAFHAAREVDLEALKLNEVIVSQPYTPL
                     VTTVVCADYSLTGEWSAAHRYARQTSIIARSDDGVPYTGIPRRLVTEALLRAGDVALA
                     EEYLDLLGQRDGDGRRDRIEYLQACAGLARWQGQLEQALTHLEEARTLAEEIGLPGEL
                     WQIEAALADLYQSRGEQVLASQAYIQAAAVVRDLTGTIEDEALRASFLAAAQVRRVME
                     YGA"
     gene            complement(5143..>5927)
                     /locus_tag="VGT44_22525"
     CDS             complement(5143..>5927)
                     /locus_tag="VGT44_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583653.1"
                     /translation="IEYYEQAWQLLAEQPVGYAMQAKFPAPDVQQLFFSLGRAYELTR
                     TWEHGRHMFGAMLERLLQMAAQDDSANGLAKAQWYRAMLDMYTADQAVAIAHGERALA
                     LARSIEQSDLIVQSLDALAHLKMRLGAWEENEQLVAEARALYAAMRDRAMEADCLCLL
                     ANAHLHRGRPPAGIAQARNALTISQEIENTWGQVNAIHEVTSGLLDIGAYTEALESAL
                     RAVARARTQPALIAGELVASSQSHSIGRRLPGNPGVPCCTRG"
BASE COUNT         1267 a         1698 c         1613 g         1349 t
ORIGIN      
        1 cgtcatggtc gtcccattga ctgcgaggtt gcctggatag ctggggatga ggcccaccag
       61 gaaggtaatc aggtagaaga cggcgcctgc aagagcgcct gccaggaagc cgagccgacg
      121 acggacggca attctgccca cgatcaaacc gacaacaaag ccaatgagca ggctcagcgt
      181 gacagtgagg agttccaatc ctgccagggc aagagccgtc ttgacagtca acttgtccac
      241 tgcaatttgc tggctggctg catgaaaggt gcctgcaaac accagcgtaa ccaccaacgt
      301 cagcagagca gtgatcaccc ctgcaatgac accggcaatt gctgcatagt acaatcccct
      361 ttgagggagc aaagcatgtt gctgagcgcg tgggccactc ggttgtatcg gagacggcgt
      421 tcctgctacc ggagtttggc cttgaagcgt ctcagttgat tgatcgtgtt ctgttgattc
      481 gaggcctcgc ggttccggtg gctcactttg gatgggtggg aggtctccag tgctgtggag
      541 agtgcgcagt tcgctctgcg tggccgcgtt ttgcataatg gttctccttg tccctcagct
      601 catcatcccc gatgtcaggc agtacgactt cggccgcgcc ccagacccaa cgccaggagc
      661 tgccatatcg ccaccaggat gatcgcgccg accagcgctt tgaaaatggg gacaccatac
      721 acgttggtat caaaccacgc ccccaatccc attgatgatg atgacctgac tgagcaacca
      781 tgccccaacg agcgccgcga cgatagcgcc aacgaagccg aagggaagtc gcctgccaac
      841 aagggcctcg gccaccaggc caacgaccgc cgcgacgatc aggtaaatga taaagttgag
      901 accgaacgtc cagacattgt cacccagttt aatggtgact ccatctgcca gaatgaactg
      961 tagagtaaac atctcgtcct ccttattagg aagtgatgtc aaattcactc aggccattgc
     1021 gcctgcacat ccatccattc ccctttatcg acgtccccat cctctcgacc gggatgttcc
     1081 tccgcggctc acagcgtgta agatggcaag cagtatacag gccccgataa aggcaatgat
     1141 gatcgtgacg atcaggctac tggagcctcc gatccccagg aagctggcta accatcctcc
     1201 gatgaaggct ccgataaggc caacgacaat atcgccgatc agcccatagc ctcccccacg
     1261 catcacccgg ctggcgagaa agccggcaat caatccaaca accagccacc agagtacatt
     1321 tgacagacta agattgatat cagccagtag aagtgttgca agtgtcatga ccaggtcctt
     1381 tccttgatac cgatgtatcg ctctccgtgc tttccgtcgc tcatccagat ctactagacc
     1441 tggggaggaa agccatatac actgttttaa gtgtacttac ggagcgttat aaaaagcgtt
     1501 atccccgcgt taaatgcgtc cctctacaaa agcgacttct gtgttgattc cggatcgcca
     1561 ggtgcaagga cgtacccgtg ctcctctgta atcgacaatc ttgaaagcca tccccatttt
     1621 cccaaagatc aggagaccgc cttcgcctgt cgtaatggcg cactgacgag gtgcagagcc
     1681 tgcacgattt ccccatacga tcgccaccag ttcgtctcgt agtagggaat agagtgtgcc
     1741 aggcagaaag ctctgataat gccctgcgct ttcttcaact ggttgcgagg catacctgga
     1801 aagaggtggt gttcgatctg gtagttcagc ccaccatacc agaaatcggt aaaaggatgc
     1861 ccacgcacat tgcgcgcggt gattacctgg cggtgcaaga agtccatctg gctgtccgca
     1921 tccagtacag gcatgccttt atggtttgga gcgaagatcg accccagata gaaaccggag
     1981 agtccctggt ggacgagaat gaagaggatc gcctgccagg ccgctaaatg ggagaagagc
     2041 aggccaaagt agcacgcgaa gtgcagaccc agtaatagcc attctagagc ggcatatttc
     2101 accttttcgc gaaacaggaa ctgaatgccg ttgatctgaa gatcaaaggc gaccagcaaa
     2161 agcacgggaa agaagaagta ggcctggtat tttatgagtg cctgaaaggg ccagcccctg
     2221 cggtgaagat cgtcttcccc aaaggcaatg atcggaatat ttatgtcggg gtcgagatcg
     2281 cgttgattgg gatggctgtg gtgtttgttg tgcttatcca tccaccatgc attgctcatg
     2341 cctatcagca agttgccgac aatcagtcct acaagattgt ttttccacgt cgtgtgaaac
     2401 acctgccggt gtccggcatc atgaccgagg agtccgagct gtgtggtgac aatggccagg
     2461 taggcggcat tgatgagttg aagccagaaa gagtgcacga aaagcagaaa gagtacactc
     2521 agtgcgagca tcgccactaa cagcgccatc ttgggcgcgt aggagaccgg atgcttctcc
     2581 aaaagtcctg cctccttaat caggcgtttt aactcagcat agtcgctact ggagcgagct
     2641 atggggagcg gtgtcgggtt gtctaaatga cccatgtgat gaaatcctct aggtaacttt
     2701 cttagcaaaa tgctttattg gagtgctttc tggaggtact tgccgggggt actctctagc
     2761 ggtctggtcg tcgtatgaca acggaccttc tcttgggagg gaagaagtct tcccccaaaa
     2821 gaaatgatac tgctagagcg ttagaaaaag cgtgagaacg agcgttagtc gctcgcgctc
     2881 gagatgtcgg gatagacctg cttaaccgta tttcttatat gatgaacttt cgcaaccaaa
     2941 gtcggtcttg tcatactgag cgaagcgaag catctcaccg cctatccacc gagacccttc
     3001 gcttcgctca gagcctgccc tgaacgaagt gaaggggtga cactcgcttc ccgaacctgg
     3061 tcgtgaaaat tcatcatata atggtgaggt ctcaaacatg gagcaagtat ttgaaagggc
     3121 gagaagaagc cacatgaaga cagaacaacg ggacgagaac ccctctactg cctctgctgt
     3181 gaaggataaa ccagtcgcat catttcgccg ggtgttgaag aatcgctcgt ttttattgct
     3241 ctggttggcg cagctccttt cgcagatcgt ctttaacgcg gctaattatg gtgtcattgc
     3301 cattgtgacg gcgatcaccc actccacggt gatggtcggt ctggctattg tctcgtttac
     3361 gcttcccgcc gtgccgttta gcttactggc aggcgtctat gtcgatcacc tgaacaaacg
     3421 gctcgtactc tgggtcagca atgccctgcg cgcggtggcc accggtctca tcgtactggc
     3481 gctgctctgg gacccgcaaa cggttattcc gctctacatc ctgacgtttc ttatctcact
     3541 ggtgacacag ttctttacgc cggcagaggc atcggccatc cccctgctcg tgaacaagga
     3601 cgacctggcg cctgccctct cgctcttcag catcaccctc accatcgcgc aggcactcgg
     3661 ttttttgctc cttggcggac tcatcaccgt gctctttccg ccctttcaac tctcgctcgg
     3721 ttttattcgc gtacaggttc tctcctttga catgctcttc gtgattgtcg ctgtggtcta
     3781 tctcttctct accttgctga tcctggcgat ccccgcgcgt gctttgcagc agaagcagta
     3841 cgaggagcca gagttgctca catcgcttgg aaagcagacg tggacggtca tacaacacga
     3901 cgtgcgggaa gcgtgggcct acatccgcct ggatcgcgcg ttgctgctcg ccttactgcg
     3961 ggtctcgttt gtcagcatct tgctgctggt gatcggcgag ctagcaggac cattcgtcgt
     4021 caatgtcttg tatctgccag tccagactat gcccctgatt ttagcgcccg cgggtctcgg
     4081 cctggtgctt ggtggcctgt tcatgcctac tctcatgcga tggctgggta agagccgcac
     4141 catcgccgtg ggcagcctgg ccactgctgc tggtttgata ctcatcccgc tcggccgcct
     4201 cctctggtcg cggttggctc tcccggttcc ggggatcctc ttttatgtgg gcgcagtcac
     4261 gttttgcctg ggtatagcgc tggacatggt gaatattccc gcgcaaacga tgatgcagga
     4321 gcaagcgatc gaggaggagc gagggcgtat gttttccttc cagtcgatgt tgtataatgc
     4381 cggctcaatc cccgtcctgc tctttgccgg tgtgattgcc gacacactgg gggttgagac
     4441 cgtcatgtac gtggtagcgg cggccattct gggattcagc tggtgggcgg cgcgctatag
     4501 ccaccgcata tttatctcgt gagaatgcta aagcataacg aggaagattg ccgttatgct
     4561 ccatattcca tgacgcgccg cacctgagcc gccgccagaa aactcgcccg caaagcctca
     4621 tcctcgatcg tgccggtcag gtctcgtacc acggcagccg cctggatata tgcctggctt
     4681 gccagtacct gttctccccg tgactgatac aggtctgcga gagccgcctc gatctgccat
     4741 agttctcctg gcaagccgat ctcttcggcc agggtacggg cttcctccag gtgggtaagc
     4801 gcctgctcca gctggccctg ccaccgcgcc agacctgcgc aggcttgcaa gtactcaatg
     4861 cgatctcgcc gaccgtctcc gtcgcgctgg ccgaggaggt ccaggtactc ctcggccagc
     4921 gcgacatctc cagcgcgaag caacgcctct gttacaagcc gccggggaat ccctgtgtat
     4981 ggcaccccat catcgctgcg agcgataatc gatgtctgtc gcgcgtatcg atgcgcagca
     5041 gaccattccc cggtaagtga ataatctgca cagacgaccg tggtaactaa tggcgtgtag
     5101 ggttgcgata cgattacctc gttgagcttc agtgcctcca ggtcaacctc gcgtgcagca
     5161 tggaacgcct ggattgcccg gtagacgcct cccaattgaa tgagactgcg aagaagcaac
     5221 gagttcgccc gcgatgagcg ctggctgagt gcgcgcgcgc gctaccgccc gcagtgcact
     5281 ttccagcgct tccgtatagg cgccaatgtc tagcaacccc gaggtcactt catggatggc
     5341 attgacttgt ccccaggtgt tctcgatttc ctggctgatg gtcagagcgt tgcgcgcctg
     5401 cgcaatcccc gccggtggtc gaccacggtg aaggtgtgcg tttgcgagca ggcaaagaca
     5461 atcggcttcc atggctcgat ccctcatggc agcgtagagc gcgcgcgcct ccgcgaccag
     5521 ttgctcgttc tcctcccacg cgcccaggcg catcttgaga tgagccagcg cgtccaggct
     5581 ctggacaatc aggtcagact gttcgatgct tcgggccagc gccaaagcgc gctcaccatg
     5641 agcgattgct actgcctggt ccgctgtata catgtcgagc attgcccggt accattgtgc
     5701 ctttgccaaa ccgttggcgc tgtcatcctg cgctgccatt tgcagcaacc gctcgagcat
     5761 cgccccgaac atatggcgac cgtgctccca tgttcttgtg agctcgtagg ctcgaccaag
     5821 agagaaaaag agttgttgca cgtcgggtgc ggggaattta gcctgcatcg catatccgac
     5881 tggttgttct gccagcagct gccatgcctg ttcgtagtac tcgatgg
//
LOCUS       DASZAK010001282         4599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_460555, whole genome shotgun sequence.
ACCESSION   DASZAK010001282 DASZAK010000000
VERSION     DASZAK010001282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460555"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VGT44_22530"
     CDS             complement(<1..515)
                     /locus_tag="VGT44_22530"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2583654.1"
                     /translation="MLASVKVAGMKKNSKPGLEGIRKLREQREKQGLCTTSTFMKRSI
                     AMPGSFRIGKIAGIDIDINGSWIIILVLLTVSLATGWFPQLYPGWSTATYWVTSFISS
                     LLLFVSVLLHELAHSLVARRRGLPVKSITLFIFGGVSNIEREPTSPGVEFQMAFVGPL
                     TSIVIGIVCFLL"
     gene            complement(520..1047)
                     /locus_tag="VGT44_22535"
     CDS             complement(520..1047)
                     /locus_tag="VGT44_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583655.1"
                     /translation="MSHPIPSTVRADDLLRHLQDLRSGTYEGAMSRRAKEVVYRQGVE
                     LLRPVAVKILEEANTLFLNGTGNVQMIGPEEDGSGGLVTRFELSWPEQRAARVMRGPN
                     GPLQPVRIVVNYSQGFHHPHLSGSTAGFWPFQVTSAADAGRQKGVLAAIVELELHQRI
                     YETDWRILPISWQWE"
     gene            complement(1078..1878)
                     /locus_tag="VGT44_22540"
     CDS             complement(1078..1878)
                     /locus_tag="VGT44_22540"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2583656.1"
                     /translation="MNLGLQEKVVLVTGASKGLGKAIAEAFAAEGSRVALTARNQQEL
                     EQIVQEIEQRGGQALALAADLTNDDDVNPLVAQIIAHFGTVHVLVNNAGSIGRFAAFE
                     DLSDDDWANLFNINLFSAVRITRAVLPAMRKQHWGRIINMASESGIQPDPEMQHYSAS
                     KAAMINLTKSLSKTYAKDGILVNTISPAFIKTPLVERMLADQAKAKGITVQQAEEEFL
                     RHNRPHIELGRAGTPEEVAAAVVFLASEAASFITGTNLRVDGGSVASV"
     gene            complement(2158..3678)
                     /locus_tag="VGT44_22545"
     CDS             complement(2158..3678)
                     /locus_tag="VGT44_22545"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEV2583657.1"
                     /translation="MMTTYRKHYEMLIGGERVDADKVFELVSPATEETVATVARGSVE
                     HADRAVEAARRAHEKGEWRRKSPQERATVIKSMAARMRDRLDEFVKLEALENGVPIRQ
                     ARAFHIGYSIAHLDYFGELALRYPFEQSGPQLVSPTLANGTIRREPLGVCVGIVPWNF
                     PLLLAVWKLGPALAAGNTCVMRPDSLTPLTLLELARIGEECGLPPGVLNVVTGEGAQV
                     GARLAAHPDVRKVAFTGSTAVGREVMRLASENVKKITLELGGKGPNVVLDDADVKTAV
                     DGALFACFMHSGQACESGTRLLLPDSLHDQFVARMIERARTIKIGDPFDAQTDLGPVI
                     SRKQRERIMGYIELGQKEGATLAFGGSVPRGDRFEKGFWVEPTIFTGVRNDMRIAQEE
                     IFGPVISVIRYSDVDEAVRIANATEYGLSAGVWSSDTARALEVANQLEAGTVWINDWH
                     MVSSAYPFGGYKQSGVGRELGPHALDEYTEAKFIHLDLSGRLERRAYGLLLSTPAS"
     gene            complement(3774..3956)
                     /locus_tag="VGT44_22550"
     CDS             complement(3774..3956)
                     /locus_tag="VGT44_22550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583658.1"
                     /translation="MIDPPKTAQQQDRSPDTRMMPSTFDPAYVQGAVKPFFLSAIYQG
                     ERPLLPMIDLTLSDVA"
BASE COUNT          899 a         1377 c         1342 g          981 t
ORIGIN      
        1 agcaggaaac atacgatgcc aatgacaata ctggtgagtg gtccgacaaa tgccatctgg
       61 aactccacgc ctggactcgt tggctcacgc tcaatattgg agacgccacc aaagatgaac
      121 agggtgatac ttttcacggg caacccacgt ctgcgtgcaa ccagcgagtg ggccagttca
      181 tgtaagagca cggagacaaa cagcagcagg gacgagatga agctcgtgac ccagtaggtg
      241 gcggttgacc acccgggata caactgggga aaccaaccgg tagcaaggga caccgtcagc
      301 aagaccagaa tgatgatcca gctgccattg atatcgatgt cgattccggc aattttgcca
      361 atacggaacg aaccaggcat ggcgatactc cttttcatga aagttgacgt cgtgcagaga
      421 ccttgttttt ctctttgctc gcgcaatttg cggatgccct ctaagccggg cttgctgttc
      481 ttcttcattc cggcaacctt taccgaggca agcatgctct cattcccact gccaggagat
      541 gggcagaata cgccaatcgg tttcatagat gcgctgatgc agttctaact cgacaatggc
      601 agccaggacg cctttctgac gcccggcatc ggccgcgctg gtgacttgaa acggccaaaa
      661 gcctgcggta cttccagaga ggtgaggatg gtggaagccc tgggagtagt tgacgacaat
      721 acgcaccggc tgaagcggcc cgtttgggcc ccgcatgact cgtgccgctc gctgttcagg
      781 ccagctgagc tcaaagcggg tcaccagacc gccagagcca tcttcttcgg ggccgatcat
      841 ctgcacattt ccagtgccgt ttaagaacaa ggtattggct tcctctaaga tcttcacggc
      901 gactggcctg agcagttcga ctccttggcg atataccacc tctttcgcac ggcggctcat
      961 tgctccctcg taggtgccgg accgcaaatc ttgcaggtga cgaagcagat cgtcagcgcg
     1021 gactgtggac gggatcggat gactcatgag gcgctccttt cctggaatag ctctgcttta
     1081 cacactggca acggaaccgc catcaacgcg cagattggtc ccggtgataa acgaggcggc
     1141 ctctgatgcc agaaagacga ccgctgctgc cacttcttca ggcgtccccg cgcgtccaag
     1201 ctcgatatgt ggtcgattat gtcgtaaaaa ttcttcctcg gcttgctgta ctgtgatccc
     1261 cttcgctttg gcctggtctg ccaacatacg ctcgacgagt ggcgtcttga taaaggccgg
     1321 ggagatggtg ttcaccagaa taccatcttt ggcgtaggtc tttgaaaggc ttttcgtcag
     1381 attgatcatg gccgccttcg aagcgctgta gtgctgcatc tcaggatcgg gctgaatccc
     1441 agactcggac gccatgttga tgatacgtcc ccagtgctgc tttcgcatcg cggggagcac
     1501 ggcgcgagtg atgcgcacgg cggaaaagag gttgatgttg aacaggttcg cccagtcatc
     1561 atccgagagg tcctcaaatg cggcgaacct gccaatgctt cctgcattgt tgacgagcac
     1621 atgcacggtc ccgaagtggg ctatgatctg ggccaccagg gggttgacat cgtcgtcgtt
     1681 cgtgagatct gcggccagtg cgagggcttg accgccgcgc tgctcaatct cctgtacaat
     1741 ctgttcaagc tcttgttggt tgcgtgcagt gagagccacg cggcttccct cggcggcaaa
     1801 ggcctcggcg atggccttgc ccagtccctt gcttgcccct gtcacaagca ccactttctc
     1861 ttggagtccc aggttcatct tgttcttcct ttctaatctt caatcaggtg agggccttta
     1921 ggcctcacca tattatcccg ggctaaacct acgctaaggt cagtaatgag ttaccatgag
     1981 ttgtgttgtt cctgccagag atgcaaaagg tgtttctcag ccctttcttg aagtgctgaa
     2041 ccctttcaac acatcttcga tagatcagcc tgtgaaggca ttctacggct tagcgtctgt
     2101 ttcacccgaa aaaatatcgt gaccccctta tgatacatca tctccgctcg cagccagcta
     2161 gctcgccggt gtgctcagga gaagcccgta ggcgcgacgc tcgagcctgc ccgataggtc
     2221 cagatgtatg aacttcgcct ccgtgtattc gtcaagcgcg tgcggtccca gttcacgccc
     2281 gacgccgctc tgcttgtagc ccccgaaggg gtacgcgctg ctgaccatgt gccagtcgtt
     2341 gatccagacg gtgccggcct ccagctggtt ggcgacttcg agcgcccgcg ctgtgtcaga
     2401 actccagact cccgcggaga gcccgtactc ggtggcattg gcgatgcgca cagcctcgtc
     2461 cacgtcagaa tagcggatca ctgagatcac cgggccgaag atctcctctt gtgcgatgcg
     2521 catgtcgttg cgcacgccgg tgaagatcgt cggctcgacc cagaaaccct tctcgaacct
     2581 gtcaccccgc ggcaccgagc cgccaaaagc cagcgtcgcc ccctccttct ggccgagctc
     2641 gatgtagccc atgatccgct cgcgctgctt gcgcgagatc accggcccca ggtcggtctg
     2701 ggcgtcgaac gggtcgccga tcttgatcgt cctggcgcgc tcgatcatgc gcgcgacgaa
     2761 ctggtcgtgg agcgagtcag gcaggagcag gcgggtgccc gactcgcatg cctggccgga
     2821 gtgcatgaag caggcgaaca gcgccccgtc gaccgcggtc ttgacgtcgg cgtcgtcgag
     2881 cacgacgttg ggccccttgc cgcccagctc gagcgtgatc ttcttgacgt tctcggacgc
     2941 caggcgcatg acctcgcgac ccacggcggt cgagccggtg aaggcgacct tgcgtacgtc
     3001 gggatgcgca gccagccgcg ctccgacctg ggcgccctcg ccggtcacga cgttgaggac
     3061 accgggcggc agcccgcact cctcgccgat ccgcgcgagc tcgaggaggg tgagcggggt
     3121 cagcgagtcg ggcctcatca cacaggtgtt cccggccgcc agcgccggcc ccagcttcca
     3181 gacggccagg agcagcggga agttccaggg cacgatcccg acgcacaccc cgagcggctc
     3241 gcgcctgatc gtgccgttcg ccagcgttgg ggagacaagc tgcggacctg actgttcgaa
     3301 tgggtagcgt aacgccaact cgccgaagta atcgaggtgc gcgatggagt agccgatgtg
     3361 gaaggccctc gcctggcgga tgggcacgcc attctcgagt gcctcgagct tcacgaactc
     3421 gtccaggcgg tcgcgcatgc gcgctgccat ggactttatc accgtcgccc gctcctgggg
     3481 agacttcctc ctccactcgc ccttctcgtg ggcgcgcctg gccgcctcga ctgcccgatc
     3541 agcgtgctcg acgctgccgc gcgcaaccgt ggccacggtc tcctcggtgg cggggctaac
     3601 gagttcgaag accttgtcag cgtcgacccg ctcgccaccg ataagcatct cgtagtgttt
     3661 tctgtatgtt gtcatcatac gcctcagttc ctctcaactt tgatgagtta aataatgttt
     3721 gcaacagaaa cgtagcaggc tcatagtctc cttcttatcg ggacagtagt gtactatgct
     3781 acgtcgctaa gggtgaggtc gatcatggga agcaacggcc gttcgccctg atagatggcg
     3841 ctgaggaaaa acggcttcac ggcgccctgg acataggccg ggtcgaaggt ggacggcatc
     3901 atcctggtgt cgggagacct gtcctgctgt tgggcagttt tcggtggatc aatcatgttg
     3961 tgctcctttt ctctctttta agatctcaac tgggctgcca tggctgctct gttgcacttc
     4021 ctgacagctt cggtgagagc aggcctctgc ctgagaaggt tgctcaccca cgttctgggt
     4081 gggccatgca cttgcactgc gagaagtata aaaggaggcg aaggcgaagt caccctccat
     4141 cagatggcat gtcgcttctc acctcacatt gcttgcctgc tgacaggcag ataccaaccc
     4201 cgcgtgagcg agaggctcgt cgacagtccg aaaatctgct atgatagagg acgagaggag
     4261 aaaaggttgt atggggtggc ttgaccgtgc ccccatacaa ccctagacag gcgttcaccc
     4321 acgtagcggg cacacccgat acgatcagta tagccgatgg gtcctgggga agtcaccgtc
     4381 catcggatga cgtgtcactt cacatcactg catcgtgttt cactccgctg ggtcacgcag
     4441 aatggagatc gctcgtgaag agatactcct acagtgagcg agactatgcc ttcggtcagt
     4501 tgatgctgac cctgcgcacc accatcggtc tgacgcaagc gggcctgggc gacctgctgg
     4561 gggtctcccg gcgcgcggtg gcgcagtggg aggcaggca
//
LOCUS       DASZAK010001283         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_460561, whole genome shotgun sequence.
ACCESSION   DASZAK010001283 DASZAK010000000
VERSION     DASZAK010001283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460561"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="VGT44_22555"
     CDS             <1..1995
                     /locus_tag="VGT44_22555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583659.1"
                     /translation="TYVGTLRLIAFSDALNDLGASVFSFVVLLPVLFLCYFTFCAIGP
                     WFTLGRVTRLLRALWYALTKRQVGMSGLRRALVDAFWDDPTDVELLEEPLLSWHLARP
                     GLLLAALLMAGLTTTLGVLVIQSLANWVQFGHHLLVPEAVEPSIPVGSSLVDWFPLNL
                     TVLDLSANGAVALVILLVGYSIVRHGILIERSLARRGFFEQWRGIVIVATATAIFIVL
                     LVNFTRSSLGALLLITSLATVAYALFTWSSYTAHDRYIALLGPFVRSTSLRHWLNTNQ
                     QKTEQDLENLFFHLCHDVLAVRFAYLTIMAGSVRRNFSYRWPVETMLELVLPKKRNEP
                     VDGLSSRSRIKEDGIAPEAPKRVHITLHGQPMICWVLPIYDELGLVATLYLGPREDGG
                     AFTDEDMNLAHACGQRILDTLGDHEAMQAVAGLLRRRIVDVKLLGAQQRRVLHDEILP
                     QMHLALLRLETLRSLPDANANPPLSLESAELCREQALNEVIDQISGAHRRLANMMRTT
                     APGAPHRLERDGMMHAIHTMLEQDFQNAFDEVEWCVSEETAACIDEVTPPAIAELIFA
                     AVQEALRNAARHARGSDVHRRLHLILKASFSREAPYLEVIVADDGVGIVSASGSTTGT
                     GGGLLTHNALLAIAGGSLTVKSAPGEGVTVRILLPAASLR"
BASE COUNT          391 a          605 c          541 g          512 t
ORIGIN      
        1 acctatgttg gcacattacg gctgattgcg tttagcgatg ctctcaatga cctgggcgcc
       61 agcgtgtttt cattcgttgt gctattaccc gtattgtttt tatgctactt caccttttgc
      121 gccataggcc cctggtttac acttggccgt gtaactcgtc ttctccgtgc cctgtggtat
      181 gccctgacaa aacgccaggt aggcatgtcc ggcttgcgcc gtgccctggt cgatgcattt
      241 tgggatgatc caaccgatgt cgaactattg gaggagccac ttctctcctg gcacctggct
      301 cgccctggat tgttattagc agcgctgctc atggctggcc tcacaactac gctcggtgta
      361 ctggtcatcc agtcgctagc gaactgggta caattcggac atcacctcct cgtgcctgag
      421 gctgtcgaac cctccatccc cgtagggtcg tctcttgtgg attggtttcc tctcaacttg
      481 acagtactgg atctgagcgc taacggggca gtggcgttag ttatcctgct cgttggctat
      541 tcaatcgttc gtcatggaat cctgatcgaa cgctctctgg cccgccgggg tttcttcgag
      601 caatggcggg gtatcgtgat tgttgccacg gctaccgcca tcttcattgt cctccttgtt
      661 aatttcacac gcagtagtct aggtgcgctg ctgttgatca cctcacttgc aacggttgcc
      721 tatgctctct tcacctggag tagctatact gcccacgatc gctatattgc cctgctgggg
      781 ccattcgtgc gcagtaccag cctgcgtcat tggctgaata ccaaccagca gaaaacagag
      841 caagacctgg aaaatctctt ctttcatctc tgccacgatg tcttagctgt acgcttcgcg
      901 tacctcacca tcatggccgg aagtgtcagg cgcaatttca gctatcgctg gccggtagag
      961 acgatgcttg aacttgttct ccctaaaaag agaaacgaac cggtagacgg cctgagcagc
     1021 aggagccgga tcaaggagga tggtattgca cctgaagctc ctaaacgggt acacattacc
     1081 ctgcatgggc agccgatgat ctgctgggta ttgccgattt acgatgaact tggccttgtc
     1141 gccaccctct atcttggccc ccgcgaagat ggaggagcat tcacagacga ggatatgaac
     1201 cttgcgcatg cttgtggaca gcgtatcctc gataccctgg gcgaccatga agcgatgcag
     1261 gctgtcgccg ggctcctgcg ccgccgcatt gtagatgtga aattacttgg tgctcagcag
     1321 cgccgcgtgt tgcacgacga aatcctgccg cagatgcacc tggccctctt gcgcctggaa
     1381 accttgcgtt ctctccctga tgccaatgcg aatcccccat tgagcctgga atccgctgag
     1441 ctttgccgcg agcaggcgct gaatgaagtc atagaccaga tcagcggtgc ccatcgccgc
     1501 ctggcgaaca tgatgcgtac caccgcgccc ggagctcctc atcgtctgga gcgagacggc
     1561 atgatgcatg ccattcatac catgctggaa caggattttc agaatgcctt cgacgaggtt
     1621 gagtggtgcg tctctgagga gacggctgcc tgtattgacg aagtgacccc gcccgccata
     1681 gctgaactga tctttgccgc tgtacaggaa gccctgcgaa atgccgcccg ccacgctcgc
     1741 ggcagcgatg tccaccgccg cctgcatctt atcctcaagg cctcattcag ccgcgaggct
     1801 ccatacctgg aagtcattgt tgccgatgac ggtgttggca tcgtctccgc cagtggctcc
     1861 actactggta ccggtggtgg cttactcacc cataacgcct tgcttgccat cgccggtggc
     1921 agcctgaccg tcaaaagcgc cccgggagag ggcgtcaccg tgagaatcct gctgcctgcc
     1981 gcatcactac gctaggcgtt tcttatggtc tctctgtgaa gacttcaaat tgagcgattt
     2041 ttcaccgtc
//
LOCUS       DASZAK010001284         3966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_351690, whole genome shotgun sequence.
ACCESSION   DASZAK010001284 DASZAK010000000
VERSION     DASZAK010001284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351690"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1042
                     /locus_tag="VGT44_22560"
     CDS             <1..1042
                     /locus_tag="VGT44_22560"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEV2583660.1"
                     /translation="GEILGLPLALTGRRGLLARRMRSSTAAITVLALSYARRILSNRR
                     RYAGISPVRALLLSLSDVLHRPFVETLSGLAARHHIYLCASTVSPHVRVSTLHHDVKR
                     YGRKGADRVLLPAGPGVYNTAFLWGPDGSLIGTTDKVFLTKSELTTLDLTPGDLQQVR
                     AFDTAMGKVGIAISLDAFTPEYLRVLDAQGTRIVVQNDANDTPWAAPCHSWEWQPQEW
                     LNSVLGSVQDDYPNLTYNICAMQVGNFFDIAFDGQSTITMKSDDEPDPTSNFVGNDGF
                     THTVTGKPMKGSILALSPWVAVDPGAQNPAMSLAERRAALEQVGRQLLPGGPLAHQYR
                     ESVIWADVEVPL"
     gene            complement(1011..1973)
                     /locus_tag="VGT44_22565"
     CDS             complement(1011..1973)
                     /locus_tag="VGT44_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583661.1"
                     /translation="MQSVQRLLHELGLPEGDRHDLPTSVLTFPDGAQYRVEIPSVEGP
                     AAFRAIIEASQERGILIHRISQGSGIMLLSDAEIGEMAQLGKQYGIEVCLFVGPRAPC
                     EGTAQALTPDGKHFGWRHTGMDQLIYAYNDVERAVRLGLRSVLVADEGLLWLINQARQ
                     RGLLPSNLVIKASALLGASNPLGIKLLVDHGLNTINVASDISLARLAALRQVISIPID
                     LYIESPDGLGGFMRYHEIAEIVRVAAPIYLKFGLRNAPNIYPGGIHLEQSAILTARER
                     VRRAALGLEMLARSAQDFAASQAGAPGLGIPELPVTGEPPHQPI"
     gene            complement(2035..3231)
                     /locus_tag="VGT44_22570"
     CDS             complement(2035..3231)
                     /locus_tag="VGT44_22570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007922882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HEV2583662.1"
                     /translation="MKIVDVKTYVLGTAWRNLTFVEVITDDGLTGVGEVRMLNHTDAL
                     RGYLAEAVPNHVLGHDPANIEDLVQRMYRNDYARADEIAMSAIATIEIACWDILGQAQ
                     GKPVYQLIGGAVRDRIKAYANGWYTVERTPGEFHQAARRAIEKGYRALKLDPFGAGFY
                     ELDRAEKNKAVALVEAVRDAVGQDTEILIEMHGRFNPATAIAIARELEPFNPGWIEEP
                     VPPENLAALKKAADKIASPVATGERLHTRYDYRPLFELQAADIIQPDITHFGGLLETK
                     KLAAWAEIYYILVAPHNVGGPVSTAVALHFAASTPNFKIQEHFNDFAEAWVKAAAPGN
                     PEVVDGYFSLPQGPGLGVRLNKDVVREHPQQRIFFNLFAENWHLRQAIQPTVQQEAIP
                     ANDEAN"
     gene            3321..>3966
                     /locus_tag="VGT44_22575"
     CDS             3321..>3966
                     /locus_tag="VGT44_22575"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEV2583663.1"
                     /translation="MTIKGNRVKTFSSLQRQSLSGQVVREIGLSIMRSDLKPGDALLN
                     EPELSLQFNVSRPVLREALKMLSAKGLIESRPKTGTRVRPREGWNLLDPDVLAWQHDV
                     GPDKNFLEAICEIRLMFEPPTAGLAALRASEDEILRIKQSCQSLQDAVDSPDRYIPAD
                     LQFHAAICSASHNELLQKIMDTVDTSLRASRATTSQLPDANQKAMQLHWAITEAI"
BASE COUNT          837 a         1095 c         1127 g          907 t
ORIGIN      
        1 gggcgagatt ctggggctgc cgctcgcttt gacgggaagg cgcggcctgc ttgcccgtcg
       61 aatgcgctcc tccaccgccg cgattaccgt gctggcgctg agttacgcgc ggcgcatcct
      121 gtcgaatcgc cgccgctatg ctggcatttc gcccgtgcgc gcattgctgc tttcgttgag
      181 cgatgttctg catcgcccct tcgttgaaac gctgagcggg ctggctgctc gccaccacat
      241 ctatctctgc gccagcacgg tttcgccgca cgtgcgcgtc tccacgctgc atcatgatgt
      301 caaacgttac ggccggaagg gtgcggaccg ggtgttatta cccgctggac ctggcgtcta
      361 caataccgct tttctctggg ggccagacgg cagtctcatc ggaacaaccg acaaagtctt
      421 tttgaccaaa agcgaactga ccacgcttga tctgacgcct ggcgatttgc agcaggtgcg
      481 tgcgttcgat acagcgatgg gtaaggtcgg cattgcgatc agcctggacg ccttcacgcc
      541 cgaatatctg cgcgttttgg atgcccaggg gacgcgcatc gtcgtccaga acgatgcgaa
      601 cgatacgccg tgggccgctc cctgccattc gtgggaatgg cagccgcagg agtggctgaa
      661 ctcggtgctt ggctcagtac aggatgacta ccccaacctg acttacaaca tctgcgcaat
      721 gcaggtcggc aattttttcg atatcgcctt cgatggtcaa tcgaccatca cgatgaaaag
      781 tgacgacgag ccagacccta ccagcaattt cgtgggcaac gatggcttta cccataccgt
      841 gacgggcaag ccaatgaaag gcagcatact cgccctatcc ccctgggtgg ccgtagaccc
      901 cggcgcgcaa aatcctgcga tgtctcttgc cgagcggcga gctgctctgg agcaagttgg
      961 gcgacaactg ctgccgggcg ggccgctggc gcatcagtac cgggaatccg ttatatgggc
     1021 tgatgtggag gttcccctgt aacaggaagc tcaggtatgc ccaatcctgg cgcgccagct
     1081 tgtgaagccg cgaaatcctg cgcggatcgg gcgagcattt cgagtcccag cgctgccctg
     1141 cgtacacgct cgcgcgctgt taatatggca gactgctcga gatggatgcc gcctgggtag
     1201 atattcggcg cgttccgcag gccgaacttg aggtaaattg gcgctgctac gcgcacgatt
     1261 tccgcaattt catgatagcg catgaatccc cccagaccat cagggctttc gatgtaaaga
     1321 tcaatcggta tcgaaataac ctggcgcagg gctgctaaac gtgccaggga aatatcagag
     1381 gcaacgttga ttgtgttcag gccgtgatcg accaaaagtt tgatgccgag cggattggat
     1441 gcgccaagca atgccgatgc ttttataacg aggttcgagg gcagtagacc tcgctggcgg
     1501 gcctggttaa tcagccacag cagcccttca tcggcgacaa gcacactgcg taggcccagc
     1561 cttacggcgc gctcaacatc gttgtatgcg tagatgagct gatccatgcc ggtgtgtcgc
     1621 cagccgaagt gcttgccatc aggggtcaga gcctgcgctg tgccttcaca gggcgcgcgt
     1681 gggccgacga aaaggcaaac ttctatgccg tactgcttgc cgagctgcgc catttcgccg
     1741 atttcggcat cgctcagtag cataataccg ctcccctgcg aaatgcgatg gataaggata
     1801 cctcgctcct gagaagcttc gataatggca cggaatgccg ccggtccttc gacgctcgga
     1861 atctctacac ggtactgcgc tccatctggg aaggtcaaca cagatgtcgg cagatcatga
     1921 cgatctcctt caggaagccc cagctcgtgc aataaccttt gcacactctg catgattctg
     1981 gtctctttct gcatgtttgc cgttcaatgg gaacgagtga tgaaaattgt cgatctaatt
     2041 tgcctcgtcg ttcgcaggga ttgcctcctg ctggacggtg ggctggatag cctggcggag
     2101 atgccagttc tccgcaaaaa ggttgaaaaa gatgcgctgc tgggggtgtt cgcggacgac
     2161 atccttattc agtctgacac ccaggccagg tccctggggc agcgaaaaat atccgtcgac
     2221 aacctccggg ttgcccggag cggcggcttt cacccatgcc tcggcaaagt cgttgaaatg
     2281 ctcctgaatt ttgaaattgg gggtggaagc tgcaaaatgt aacgcgacag cggtcgaaac
     2341 tggccctccc acattatgtg gcgcgaccag gatatagtat atttctgccc aggcagcgag
     2401 ttttttcgtt tcgagcagac cgccaaagtg cgtaatgtcc ggttgaatga tatcggcggc
     2461 ctgtaactcg aagagtgggc gataatcgta gcgggtatgc aggcgttcac ccgtggctac
     2521 ggggctggcg atcttatcag ccgctttttt cagagcggcc agattctcag gtggaacagg
     2581 ttcctcgatc cagccaggat tgaacggttc cagttcccgc gctatcgcta ttgccgttgc
     2641 gggattgaaa cgcccatgca tctcgatcaa aatctcggta tcctggccga cggcatctcg
     2701 caccgcctct accaatgcga ctgctttgtt tttctcggct ctatcgagtt cgtaaaagcc
     2761 tgctccgaat ggatcaagct tgagtgcccg gtatcccttc tcaatcgccc gcctggctgc
     2821 ctggtggaat tcgccggggg tgcgttcaac cgtataccag ccgttggcgt acgccttgat
     2881 gcgatctcga acagcgcctc cgatgagttg atagaccggc ttgccctggg cctggcctaa
     2941 gatgtcccag caggcgatct cgatggtagc gatagcggac atggcgatct cgtctgcgcg
     3001 cgcgtagtcg ttgcgataca tgcgctgcac caggtcttca atattggccg ggtcgtgtcc
     3061 tagaacatga ttcggcaccg cctccgccag gtagcctcgc aatgcatcag tgtggttcaa
     3121 catgcgtact tcgccgaccc ctgttaatcc gtcatcagtg atgacttcca caaaggtcag
     3181 gttgcgccag gccgtaccca gtacgtaggt tttgacatca acaattttca tgctcgctcc
     3241 ttgtcgggaa tttggttagc aaaccacttg attagggaca gatactcatg tattatataa
     3301 ccattataag gtattattgt atgacaataa agggcaacag ggtgaaaact ttttcttcgc
     3361 ttcagaggca gagcctctca gggcaggttg tgcgtgagat tggtttgagt attatgcgga
     3421 gtgacctaaa accgggcgat gctttattga atgaacctga actcagctta cagttcaacg
     3481 tcagccgacc ggtgctgagg gaggcattaa aaatgctgag cgcgaaaggt ttgattgaat
     3541 cgcgtccgaa gacgggtacg cgggtgcgcc ctcgcgaagg ttggaacttg cttgatcctg
     3601 atgtactcgc ctggcagcac gatgtgggac ctgataagaa cttccttgag gcgatttgtg
     3661 agatccgact catgttcgag ccaccaactg ccgggcttgc ggcgttacgc gcttccgaag
     3721 acgaaatact acggatcaag cagagctgcc agagtttgca agacgcggtt gactctccag
     3781 accgctacat ccctgctgat ttacaatttc atgcggcaat ctgctcggcg tcccataatg
     3841 agctgttgca aaagatcatg gacacagtgg atacatcctt acgagctagc cgggctacta
     3901 catcgcagtt gccagacgcg aaccagaagg caatgcagtt gcactgggca attaccgagg
     3961 cgattg
//
LOCUS       DASZAK010001285         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_423866, whole genome shotgun sequence.
ACCESSION   DASZAK010001285 DASZAK010000000
VERSION     DASZAK010001285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423866"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1515)
                     /locus_tag="VGT44_22580"
     CDS             complement(28..1515)
                     /locus_tag="VGT44_22580"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEV2583664.1"
                     /translation="MNDVHSPIATRARAARLHEGAIGPWGLAALAIGITSPAMGLYAL
                     WGPMQVATGPITPLVFIAAMILVLPTALSYAALNSRAPSAGAASNWLWRSLSPVVGYQ
                     AGLAMSAYFIMAAISQPLLFALFFRDLIAYLAIPIPATFVPVIGVCVGTIPVAAVTLK
                     GAGLSVKTTVGLMLIESLVVVGLSATIFMHKVPEPGAAYLAAFDPRAATHGMSGFWAA
                     MILGAMAFAGFDVVSTAAEETRAPREFIPKTILLTVIGIAVFWAVNAWAFTLAIPHSV
                     VTQYTLQGLTPVTPVARAYWGGGSIIVIATALTGITAVYISCIQGSSRLIFALARHGL
                     LPAALGELSGTKRVPRNAVLAILGLVLALDVVTLLILRNCLEGFTWWANALVFFAVIT
                     FFGVNVANISHFLRIEPDRFHVLRNLLLPLAGAAMNLYLLYAAFFGSLWSAPLRTGRS
                     VVIACVALFIVQMGSVAYVRWNRAELLSQDAPLGADASPAAAEGA"
BASE COUNT          434 a          587 c          585 g          308 t
ORIGIN      
        1 tcgccgtttg atcttgtagg tcgattttca tgcaccctcc gcagctgccg ggctcgcatc
       61 cgcccccagg ggcgcgtcct gcgataacaa ctccgctcga ttccagcgca catatgcgac
      121 gcttcccatc tggacgatga acagtgcgac gcacgcaatc accacgctcc tacccgttcg
      181 caggggggcg gaccacaggg acccgaaaaa ggctgcgtac agcagatata gattcatcgc
      241 cgcgcctgcg agcggcaaga gcaggttccg cagcacatga aatctatcgg gctcgatgcg
      301 cagaaagtgc gagatgttgg ccacgttcac gccgaaaaac gtgatcacgg caaagaacac
      361 cagggcattc gcccaccatg tgaacccttc caggcagttt cgcaggatga gaagcgtgac
      421 cacatccaac gccagcacca ggcccaggat cgcaagcact gcgtttcgcg ggactcgctt
      481 ggtccccgac agctcgccca gcgcggccgg cagcagccca tggcgcgcga gcgcaaatat
      541 cagccgcgag ctgccctgaa tgcagctgat atagacagcg gtgatgccag tcaaagcggt
      601 ggctatcacg atgatacttc cgcctcccca gtacgcccgc gccacaggcg tcacgggcgt
      661 caatccctga agtgtgtact gcgtaaccac ggagtggggg atcgccaacg tgaaggccca
      721 ggcgttgacc gcccaaaata ctgcaatgcc gatgaccgtc agcaaaatcg tcttgggaat
      781 gaattctcga ggcgctctgg tttcttcggc ggccgtggat acgacgtcga acccggcgaa
      841 ggccatcgcg ccaagtatca tcgcggccca aaagcccgac atcccgtgtg ttgcggcgcg
      901 cggatcgaag gccgcgagat aggccgcgcc gggctcaggc accttatgca tgaagatagt
      961 cgctgacagt cccaccacga ccagggactc gatgagcatc agccccaccg tggttttgac
     1021 ggatagccca gcgcccttga gagttactgc agcgacgggg atcgtgccga cgcagacgcc
     1081 tatcaccggc acgaaggtcg cggggatcgg aattgcgaga taggcgatca ggtcccggaa
     1141 aaacagcgca aaaaggagcg gctgcgaaat cgctgccatg atgaaatacg cgctcatcgc
     1201 gagaccggct tgatagccga cgacgggact gagcgacctc cagagccaat tcgaggcggc
     1261 gcccgcggag ggggcgcggc tgttcagcgc ggcgtatgag agcgcggtgg gcagcaccag
     1321 aatcatcgcc gcgatgaata ccaacggcgt gatcggaccc gttgcgacct gcatcggccc
     1381 ccacagcgca tagagaccca tggccggtga agtgatgccg atcgcaagcg cggcgaggcc
     1441 ccaagggccg atggcgcctt cgtgcagacg cgccgctctg gcacgcgtgg caatcgggct
     1501 atggacgtcg ttcatagagt ctcacagatt ggatccaatt tgatcttaag ggatccatgc
     1561 tcccagcgct agcgcgatgc cgcgggccgg ggcgaaagga ttgcgccgag acctccgtcg
     1621 catggattga cgcacggtaa tcggttcgcg aggcaaaggt acaaatgaaa tcagatactt
     1681 gaacaaattc tgccatttgc cgaccgcgac ggcacatcgg cttcccagtg caccccgcgg
     1741 gcggtatctg tatccaaaag gcgctgcgcg atagactgcg cagattgagc agatcgatgg
     1801 actgtgggca gaaccatgca aagaaaacgt gcgaaaggcc ccgagcaggg actgatgggg
     1861 cagcaggtca ccgaaggcat ccgcgatctg atcttatccg ggcagctacc accc
//
LOCUS       DASZAK010001286         7411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_104940, whole genome shotgun sequence.
ACCESSION   DASZAK010001286 DASZAK010000000
VERSION     DASZAK010001286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104940"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1069)
                     /locus_tag="VGT44_22585"
     CDS             complement(41..1069)
                     /locus_tag="VGT44_22585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583665.1"
                     /translation="MSENVFVTLDSKYTISVPQGLEQAYDPDMQQAMDRAFALRHEHF
                     SDDITFHAPGLRRYKTAEYTNQDHRRFVSISLTGAQCALACDHCNMSILKSMASQPKL
                     GGSLFEMCSELACQGATGVLISGGSDKKGRVPLLKHIPDLIRVRRELGMTIRVHPGIP
                     DEETVAGLAQVGIDGAMMDIIGDNDTIRDVYHLAGTTTEHYERALALLDSYNVPSIPH
                     IILGLHYGQMRGEQTALEIIARHNHQAVVLVILMPLSGTPMVHITPPPVEEIMRFFAH
                     TRMTLPESAVMLGCARPAGKSKLLTDRAAVDAGLNGIAYPAEGIITYARQRGLQPKLI
                     NACCGVQW"
     gene            complement(1066..2196)
                     /locus_tag="VGT44_22590"
     CDS             complement(1066..2196)
                     /locus_tag="VGT44_22590"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV2583666.1"
                     /translation="MNEWRLIKDDGVSASFGLAADECLAHRVGTGTSQPTLRLYTYRS
                     HCALVGRFQNIDNEINRAYCEVNGISLNRRPTGGGAIIMGQDQLGIALCSPGREEDSY
                     RHARELMVHFSAGIISGLEALGIQAAFWGKNDIEIHHRKVAGLGVYRAPEGGLLFHAS
                     VLVDIDIAFMLHVLKTPFEKITDKAIATVADRVTTIRRETASSVALDEIRANIAAGYR
                     RTFGVSVAPGNFLPDELEAIDALEKSKYLTPDWVNQTTAIPDTFGSAKAKTPAGLLDV
                     RATMAGNTIKAIFIGGDFFAAEGAVADLEARLRWHPASPERVRETLAAGYAEHANQLN
                     NLPLEALQEAIRQAVRDAQKAESAARADPYGCFVTPQDKKST"
     gene            complement(2193..2621)
                     /locus_tag="VGT44_22595"
     CDS             complement(2193..2621)
                     /locus_tag="VGT44_22595"
                     /inference="COORDINATES: protein motif:HMM:NF013743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEV2583667.1"
                     /translation="MTVERQQYHLPSELYYESQTRLWVRVDGEIATIGLSALALETFG
                     DIAYISTIKAGKPVERGQVIGSIEAAKMVDDLIAPISGEIIAFNEEVQRHPGFINVDP
                     YGGGWLVRVKPSAWDRESAALIHGPALEPWIKEQLGRLEQ"
     gene            complement(2743..5559)
                     /locus_tag="VGT44_22600"
     CDS             complement(2743..5559)
                     /locus_tag="VGT44_22600"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2583668.1"
                     /translation="MTEIPLQHHIAPSGEQITVRDLASCPPPERWHDWVEYDAKAWPR
                     KVEKHYAIIPTICFNCEAACGLMAYVDKETGRVKKFEGNPYHPGSRGRNCAKGPATIN
                     QVNDPERILYPMKRSGKRGEGKWERTTWDEVLDTFATKIRAAIIDDRRDEVMYHVGRP
                     GHDGYMERVLGAWGVDGHNSHTNVCSSSARFGYQIWCGMDRPSPDHANARFILLISSH
                     LETGHYFNPQAQRIIEGKMKGAKLAVMDPRLSNTASMADYWLPTWPGSETAVLLAMAR
                     LLLAEGLYNCEFVRRWVNWQEYLAAEHPSEPQTFDHFIEVLLDLYAQYTPAFAAQESG
                     LSEEVIVDIARQIGKAGSAFSAHTWRAATAGNLGGWQVSRALWFLSVLTGSIGTPGGT
                     APNSWNKFVPAPFLKPSPSDVWNELLFPPEYPLANYEMSILLPYFLRDGRGKLAAYFT
                     RVYNPVWTNPDGTAWIEALTNEQQIELHAALTPVWSETAWYADYILPMGMASERHDLM
                     SQETHAARWIGFRQPVLRVHQERQGRPVQYTYEANPGEVWEEDEFWIELSWRIDPDGS
                     LGIRRYFESPYCPGEKLSISEYYRWIFENSVPGLPEAAEGEQLTPLEYMQKYGAFEIP
                     REKSPVFRLHERALSTTDLEQAQVNEQNGVVKRNGATIGVMIDGKACEGFPTPSRKLE
                     FYSPTLKEWGWPEYSVPGITTSHVHSSNFEHARGEYLLIPTFRLPTLIHTRSGNAKWL
                     YEISNANPVWIHPVDAARIGVETGDLVRVTTEIGHYVNRAWVTEGIRPGIVACSHHLG
                     RWRLETSEGTDRWASALVELKREDDGTWRMRHREGVRPFESDDPDSARIFWREGGVHQ
                     NLTFPVHPDPISGMHCWHQKVKVEPAHQGDRYGDVFVDTAKAYEVYKEWLALTRPQLD
                     RADGLRRPLWMIRPYRPVASAFKR"
     gene            complement(5657..7201)
                     /locus_tag="VGT44_22605"
     CDS             complement(5657..7201)
                     /locus_tag="VGT44_22605"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEV2583669.1"
                     /translation="MPIGVYRTWVKYIEKGEFPNSRRYFLVNRCNHCDNAPCVAICPT
                     RALFKRKDGIVDFDSSRCIGCKSCMQACPYDALYIDPYSHTAAKCNYCAHRTEIGLEP
                     ACVVVCPERAIIAGDLHNPDTEIARIVARQPVRTRKPEQGTGPNVYYLGAEEAAINPE
                     IAVEELPHMTMWSTLLKPNHDDEGEIDGSKSLRALKTITPLKPYVPIEPQRHTPAGAR
                     IVYDTPKNNAPWGWKVSAYLGTKSIGTGVMIVAALALILYGLSGTLSSVLNSLIGIGA
                     PVIGGFFIALTLFLLIADLKRPDRALFLVTRANPTSWLVWGGYILGIFGLIEAAWFIA
                     ALFGLKVVLLWLFIPAMLFGAAGAGYTAFLFGQAEGRDFWQSPLLLPILLVQAVIAGS
                     SALGLLAWPLNVGSSASTLLSVLLLSAITLHVFLIMIEVFGSHSNSHVAMAAHSMRSG
                     RLKTIFWGLFFASGSLLPIAMLCIALLVPTAQPALLAFSGILALVGLFAYEHCFVTAG
                     QVVPLS"
     gene            7106..7309
                     /locus_tag="VGT44_22610"
     CDS             7106..7309
                     /locus_tag="VGT44_22610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583670.1"
                     /translation="MITTVDEKIAARVGKLAFFDVLDPRSVNADGDVMFGFTRYSASM
                     TADALALVDDKSIFRQVGFPLVG"
BASE COUNT         1586 a         2041 c         2105 g         1679 t
ORIGIN      
        1 tacttttgcc atcgaagttc tccttgcatg tagctctgga ctaccactgc accccgcagc
       61 acgcattgat aagttttggt tgaagaccgc gctgccgtgc ataggtaatg atcccttcgg
      121 cggggtaggc gataccgttg agcccagcat caacggccgc acggtcggtc agtagtttgc
      181 tcttcccggc ggggcgggcg cagcccaaca tcaccgcgga ctctggcaga gtcatgcggg
      241 tatgtgcaaa aaatctcata atttcctcaa cgggcggtgg cgttatatgg accatcggcg
      301 tgccggaaag gggcatcaaa atcaccagca cgaccgcctg gtggttatgg cgtgcgatga
      361 tttccagcgc tgtttgttcg cctcgcatct gcccgtagtg cagccccagg atgatatggg
      421 gaatagatgg tacattgtaa ctatcaagga gagcgagcgc gcgctcgtaa tgttcggtgg
      481 tggtaccggc gagatggtac acgtcgcgga tggtgtcgtt atctccgata atatccatca
      541 tcgcgccgtc aatgccgact tgcgccagcc ccgcaacggt ttcctcatcg gggatgcccg
      601 gatggacgcg gatcgtcatg ccgagttcgc gccggacgcg gatcaaatcg gggatatgct
      661 tgaggagcgg cacgcgtcct tttttatcgc tcccgccgga gatcagcacc ccagtcgctc
      721 cctggcacgc cagctcgctg cacatctcga aaagcgaacc acctaatttg ggctgcgagg
      781 ccatgctttt gagaatgctc atgttgcagt ggtcgcaagc cagcgcgcat tgcgcacccg
      841 tcaggctgat cgacacaaag cgccggtgat cctggttggt gtattcggcc gttttatagc
      901 ggcgcagacc cggagcgtga aatgttatgt cgtctgagaa atgttcgtgt cgcagcgcga
      961 aagctctgtc catcgcctgc tgcatatccg ggtcgtaggc ttgctccagg ccttgtggta
     1021 cagagatggt gtatttcgaa tccagcgtaa caaagacatt ttcgctcatg tgcttttctt
     1081 gtcctgagga gttacaaaac agccataggg atcggcgcgg gcggcggatt cggctttttg
     1141 agcatcgcga actgcctgcc gaattgcctc ttgcaatgct tccagaggaa ggttgttcaa
     1201 ctggttggca tgttccgcat accctgctgc cagggtttcc cgaactcttt ccgggctggc
     1261 aggatgccag cgcagccgcg cttcgagatc ggcaacagcc ccttccgccg cgaaaaaatc
     1321 accgccgatg aagatagctt tgatggtatt gcctgccatt gtagcgcgaa catcaagcag
     1381 ccctgcggga gttttggcct tcgctgagcc gaaggtatca ggaatagccg ttgtctggtt
     1441 cacccaatcg ggcgtcaggt atttgctttt ttccagtgca tcaatggctt ccagttcatc
     1501 tggcagaaaa ttaccaggag ccacggacac tccaaatgtc ctgcgataac cggcggcgat
     1561 atttgcgcgg atttcatcca gagcgacaga tgacgcggtt tcgcgacgga tggtcgtgac
     1621 acgatccgca acggtagcga tggctttatc ggtgattttt tcaaacgggg tcttgagtac
     1681 atgcagcata aaggcaatat caatatcgac cagcacggaa gcatggaaca ataacccccc
     1741 ttcgggagcg cgataaactc ccagcccggc cactttccga tggtggattt caatgtcgtt
     1801 tttgccccag aaggctgcct ggataccgag cgcctccaaa ccgctgatga tgcctgccga
     1861 aaaatgcacc atcaactcgc gggcgtggcg gtaagaatct tcctcccggc ctggactaca
     1921 cagcgcgatg ccaagctgat cctggcccat gatgatggct ccgcccccgg taggtcgcct
     1981 gttcagcgag atgccgttca cttcacaata agcccggttg atttcgttgt cgatattctg
     2041 gaatcgaccg accagcgcgc aatgagagcg gtaggtatat aaccgcagag tcggctggct
     2101 tgtgcctgtc ccaacgcggt gcgccaggca ttcatcggca gccaatccaa aggaggcgct
     2161 tacgccatca tctttgatca agcgccattc gttcattgct ccaaccttcc aagctgttct
     2221 ttgatccagg gttccagtgc cggaccatgt atcagagctg ctgactcccg gtcccacgcc
     2281 gagggcttga ctctgaccag ccagccgccg ccgtacggat cgacattgat gaatcctgga
     2341 tgacgctgta cttcttcgtt aaacgcgata atttcaccgc taatgggggc gatcaggtcg
     2401 tcgaccatct tcgctgcttc aatcgacccg atgacctggc cgcgttcgac gggtttgcct
     2461 gccttgattg tcgaaatata ggcaatatcg ccaaaggttt ccagggcaag cgcgctcaaa
     2521 ccgatggtag caatttcccc gtcaacacgc acccagagac gggtttggct ttcataatac
     2581 agttcagacg gaagatgata ctgctgtctt tctacagtca tattgacctc actttatctc
     2641 ggctcacttt ggagcaacca caaatgattt gcgagaaatg tagctctccc acatcacatc
     2701 ttgctctcaa ttgctccctg ccgccttaca atcgactcac agctagcgct tgaaagcgct
     2761 tgccaccggg cgatacgggc gaatcatcca caacgggcgg cgcaggccat ccgcccggtc
     2821 taactgcggg cgcgtcaacg ccagccactc tttgtatact tcgtaagctt tcgccgtatc
     2881 cacaaagaca tcaccgtagc gatccccctg gtgtgcaggt tccaccttga ctttctggtg
     2941 ccagcagtgc ataccactga tggggtcggg atgcacgggg aaggtcagat tctgatgcac
     3001 accgccctcg cgccagaaaa tgcgcgccga gtccgggtca tcgctttcga atgggcgcac
     3061 cccctcgcga tggcgcatcc tccatgtgcc gtcgtcttct cgcttcagtt ccaccaacgc
     3121 cgatgcccag cggtcggtgc cttcgctcgt ttcaagtcgc cagcgaccga gatgatgcga
     3181 gcaggctacg ataccggggc gaatgccctc tgtcacccaa gcgcggttca cataatggcc
     3241 gatctcggtt gtcactctta ccaggtcgcc cgtctcgacg ccgatgcgcg ccgcgtctac
     3301 tgggtgaatc cagaccgggt tcgcgttgga gatctcatag agccacttgg cattgcccga
     3361 gcgcgtgtga atgagcgttg gcaggcggaa agtgggaatc aacaggtact cgccgcgcgc
     3421 gtgctcgaag ttcgaggaat gaacgtggct ggttgtgata cctggtacgc tatattcggg
     3481 ccacccccac tctttgagcg tcggcgagta aaactccagc ttgcgtgaag gcgtagggaa
     3541 accctcgcag gctttgccgt cgatcatcac gccgatcgtt gcaccgttgc gtttgacgac
     3601 gccgttctgc tcgttcacct gcgcctgttc caggtcggtg gttgagagcg cgcgctcgtg
     3661 caaacggaaa acgggtgatt tttcccgagg aatctcaaac gccccgtatt tctgcatata
     3721 ttccagagga gtaagctgct cgccttccgc cgcctccggc aggccgggga cgctgttttc
     3781 gaagatccag cgataatact cgctgatgct cagcttttcg ccagggcaat agggtgactc
     3841 gaagtagcgc cggataccca ggctaccgtc gggatctata cgccaggaga gttcaatcca
     3901 gaattcgtcc tcttcccaca cttcgcccgg attggcctcg taggtatatt gtaccggtct
     3961 cccctggcgc tcctggtgga cacgtagcac gggctggcga aaaccgatcc agcgcgcggc
     4021 atgcgtctcc tggctcataa ggtcgtggcg ctcgcttgcc atacccatcg gaaggatata
     4081 gtctgcgtac catgctgtct cgctccagac cggggtcagc gccgcgtgta actcgatctg
     4141 ctgctcgttg gtcagagcct cgatccaggc ggtaccgtca gggttggtcc agacagggtt
     4201 gtagacacgg gtaaagtagg cagcgagttt gccccggcca tcccgcagga agtagggcag
     4261 caggatgctc atttcgtagt tcgccagggg atactcgggc gggaagagca gttcgttcca
     4321 gacgtcgctg ggagagggtt taaggaaagg cgcggggacg aacttattcc aggagttagg
     4381 ggctgttcct ccgggcgtgc caatgctgcc ggtgaggacc gagaggaacc acagggcacg
     4441 cgagacctgc cacccaccga ggttgccagc ggtggccgca cgccaggtat gcgcggaaaa
     4501 ggcgctcccg gccttgccga tctgccgcgc gatatcaacg atcacctctt ccgatagccc
     4561 gctctcctgg gcagcaaagg cgggagtata ttgcgcgtac aggtcaagca gcacctcgat
     4621 gaaatggtca aaggtctgcg gctcgctggg gtgttcagcc gccaggtact cttgccagtt
     4681 gacccagcgc cgcacaaact cgcagttgta cagtccctcg gccagcaaga gacgtgccat
     4741 cgccagcagt acggctgtct ctgagcctgg ccaggtcgga agccagtagt cggccatgct
     4801 ggcggtattg gagaggcgtg gatccatgac cgcaagctta gcccctttca ttttgccctc
     4861 gatgatacgc tgcgcctgcg gattgaaata gtggcccgtc tcaaggtgcg aactgatgag
     4921 caaaataaag cgagcgttag cgtgatcagg cgatggccgg tccataccgc accagatctg
     4981 gtagccgaag cgtgccgagg aagagcagac gttggtatga ctgttgtgac catcaacgcc
     5041 ccacgctcct agcacgcgct ccatgtagcc gtcgtgccca ggccgcccga catgatacat
     5101 gacctcgtcg cgacgatcat cgatgatggc tgcacggatt ttggtagcga atgtgtccag
     5161 cacttcgtcc caggtggtgc gctcccattt gccttcgcca cgtttgccgg agcgtttcat
     5221 gggatagagg atgcgttccg ggtcattgac ctggttgatg gtagccggtc ctttagcgca
     5281 gttgcgccca cggctgcctg gatgataggg attaccctcg aattttttga cacggcctgt
     5341 ttccttatcc acataggcca tgagaccaca ggcggcttca cagttgaagc agatggtggg
     5401 aatgatggcg tagtgctttt cgactttgcg cggccaggct ttggcatcgt attcaaccca
     5461 gtcgtgccag cgttcgggtg gcggacagga ggccaggtct cgcacggtta tctgttcacc
     5521 gctgggtgcg atgtggtgtt gtaagggtat ctcggtcatc tctatctcca gttagatttg
     5581 tatcagttgt atcagttgta tcagttgttg cacttgtatc ggttgtattg gttgttgcac
     5641 tcctattttt cgctaatcaa ctcaatggaa ctacctgtcc ggcagtaacg aaacagtgtt
     5701 cgtaagcgaa aagaccgacc aatgcaagta tgcctgagaa ggctaatagc gcgggttgtg
     5761 cggttggcac cagtagagcg atgcagagca ttgcgatggg taggagcgaa cctgaagcaa
     5821 agaatagtcc ccaaaaaata gtttttagcc tgccgctcct catagagtgc gcagccattg
     5881 cgacatggct attgctgtgc gagccgaaga cttctatcat gatcagaaat acatggagtg
     5941 tgattgcact tagcaacaag acggatagca atgtgctggc agagcttcca acattaagcg
     6001 gccaggcgag caatcccaga gcggaggagc ctgcaatcac tgcctgtaca agcaggatgg
     6061 gaagcaagag cgggctttgc cagaaatcgc gcccctcggc ctgcccaaac aagaatgccg
     6121 tataaccagc ccctgcagcc ccgaaaagca tggcggggat gaagagccag agcagcacaa
     6181 ctttcaaacc gaatagagct gcgatgaacc aggcggcttc aatcaagcca aagataccga
     6241 ggatgtagcc tccccagacg agccacgagg ttgggttcgc cctggtgacg agaaagagcg
     6301 cgcgatcagg gcgtttgaga tcagcgatta gcaagaaaag tgtcaatgct atgaaaaagc
     6361 cgccgatgac tggtgcgccg atgccgatca aagagttcaa gacgctactc agggtaccac
     6421 tcaatccgta tagtatcagc gccaatgccg ctacgatcat aactcctgtg cctatcgatt
     6481 tcgtgccaag gtaggcagag actttccaac cccatggcgc attatttttg ggcgtgtcat
     6541 agacaatacg cgccccggca ggtgtgtgtc gttgaggttc gataggcacg tatggtttga
     6601 gaggggtgat tgttttgaga gcgcgtagcg acttgctacc gtcgatttcg ccctcgtcgt
     6661 cgtgattcgg cttgagcaat gtactccaca tcgtcatatg cggcaactct tcgacagcaa
     6721 tttcgggatt gattgctgct tcttcggcac ccaggtaata gacatttggg cctgtgccct
     6781 gctctggttt gcgcgtgcgt acaggttggc gagcgacgat cctggcgatt tcggtatcgg
     6841 ggttatgtag atcacctgca atgatggccc gttcaggaca gacaacgacg caagctggct
     6901 ctaaaccaat ctccgtgcga tgcgcacaat agttgcattt ggcggcggta tggctgtagg
     6961 ggtcaatgta taaggcgtca tatggacacg cctgcatgca agatttacag ccgatgcagc
     7021 gtgatgaatc gaaatcgacg atgccgtcct tgcgcttaaa taaggccctg gtagggcaaa
     7081 tagcaacaca tggagcatta tcgcagtgat tacaacggtt gacgagaaaa tagcggcgcg
     7141 agttgggaaa ctcgcctttt tcgatgtact tgacccacgt tcggtaaacg ccgatgggga
     7201 tgtcatgttc ggctttacac gctacagtgc aagcatgaca gccgatgcac ttgcgctggt
     7261 cgatgacaaa agcatatttc gtcaagttgg cttccctctc gttggataga agaggaatga
     7321 tacgattatt gaattgcaga cagcaccgca gctctcgaag ctagattgcg tctcagtatt
     7381 ctgagaggaa cgtatctctt ctaccagtgt a
//
LOCUS       DASZAK010001287         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_494152, whole genome shotgun sequence.
ACCESSION   DASZAK010001287 DASZAK010000000
VERSION     DASZAK010001287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494152"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VGT44_22615"
     CDS             <1..788
                     /locus_tag="VGT44_22615"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEV2583671.1"
                     /translation="CQGILSALPAEEIAQRFEQPVARARILLAGACIITAMMARLRLD
                     EITVSPHGIREGALLAYARYGERWLERVSATSTRAGRRRASTLAHMPPVDTAADGMVG
                     MNGTEPARNETFEQAGQRMLHERLQKFLEWPDEVLKNEDVEAVHKMRVASRRLRATLD
                     AFESICDPKAFKRVYRNVKKAADGLGAARDTDVMIERLRSQLAHSSSDEQPGIDWLIE
                     RLQTFRQEKQRDLGQYLHELEKIDFKRQVEACLPVKKAEAQHG"
     gene            781..1359
                     /locus_tag="VGT44_22620"
     CDS             781..1359
                     /locus_tag="VGT44_22620"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEV2583672.1"
                     /translation="MAKARPITGLDAQAPAASNARLIVKARLEDLLRWEVYVDHPYNI
                     RELHDLRIAAKRLRYSFEVFEEMLPGSCGAFVTEITRLQDELGDLHDSDVLIAMLRLS
                     LAAQDSDAVSPTGQAGQSKQHSGKTLVSSELAAHILQPDTAPTVEQRYGLELLLQRQE
                     QLRDAQYTAFRQHWYQLKARDFRREIITILDK"
     gene            1438..2079
                     /locus_tag="VGT44_22625"
     CDS             1438..2079
                     /locus_tag="VGT44_22625"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583673.1"
                     /translation="MMKPFPVEELDWWRMPQPTQEELWLSGGLRVEDWPHARQVTRLS
                     IQLFFATQPLHELDALALRLLERAAFLHNTGMLIEVRRHHKHSFSLIKEAPLPDFSDD
                     ERYEIACIARYHRRALPSMKHDEYAALSRESRKRVAALAGLLRIADALDYNHDGRVLE
                     IEADPKGCDKEHWTFSLWARPLSDLDLELEHAYEKADLFEKVFRRKVQFLVQE"
     gene            complement(2357..3286)
                     /locus_tag="VGT44_22630"
     CDS             complement(2357..3286)
                     /locus_tag="VGT44_22630"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2583674.1"
                     /translation="MLIGIDHIIVGVNDLDAAAQRFSEQLGLQASGGGVHPFGGTANR
                     VEVIGDTYLELIAVRVAGEAQRSMVERLAKGEGYLNVALGSDDLASDSEAIRRRGVAL
                     IGPTAGQLRAGDGRERGWLRSEIERPDLAQRYPFLIQHDSAGEERRSRLAGWKRPPAH
                     PLGATGVLSATIAVENLQEAARRFAHIYGLEPSIPFAGEADGWDAMLVSFPLGDSGQS
                     LELAEPIPLAAEDDAEIGLEHLPDAGALRGHLARFGESVCRMTLAVESMERARSYLDA
                     HEVTSTYVESERPALWIHPDYACGASIVLREQG"
BASE COUNT          704 a         1116 c          968 g          615 t
ORIGIN      
        1 gctgccaggg catcctgagc gcgctccctg cggaagagat agcgcagcgc ttcgagcagc
       61 cggtggcgcg cgcgcgcatt ttgctggcag gcgcgtgcat catcaccgct atgatggcgc
      121 gcctgcggct cgacgaaatc accgtcagcc cgcacggcat tcgtgagggc gcgctgctgg
      181 cctacgcgcg ctacggcgag cgctggcttg agcgcgtctc ggccacctcc acgcgcgcag
      241 gcaggcggcg cgcgtccaca ctggcacaca tgcctcccgt cgatacagcc gcagacggca
      301 tggtcggcat gaatggcacg gaacccgcgc ggaatgagac atttgagcag gccggccagc
      361 gtatgctgca cgaacggctg caaaagtttc ttgagtggcc ggacgaggtc ttgaagaacg
      421 aggatgtcga ggcggtgcat aagatgcgtg tcgcctcgcg ccgcctgcgc gccaccctgg
      481 atgccttcga gtccatctgc gacccgaaag cgttcaagcg tgtctatcgc aacgtcaaaa
      541 aggcggcgga cggcctggga gcggcgcgcg acaccgatgt catgatcgag cgtttgcgca
      601 gccagcttgc gcacagttcg agcgacgagc aaccgggcat cgactggctg atcgagcggc
      661 tgcaaacctt ccgccaggag aagcagcgtg atttaggtca gtatcttcac gagcttgaaa
      721 aaatcgattt caagcggcag gttgaggcat gtttaccggt aaagaaagca gaggcgcagc
      781 atggctaaag ctagacccat caccggcctg gacgcgcagg cgcccgccgc cagcaacgcg
      841 cgcctgatcg tcaaagcgcg cctggaagac ctgcttcgct gggaggtcta cgtcgatcat
      901 ccctacaaca tccgcgaact gcacgacctg cgcattgcgg caaaacgcct gcgctattcg
      961 tttgaagtat tcgaggaaat gttgcccggc tcctgtgggg ctttcgtcac agaaataaca
     1021 cgcctccagg atgaactggg cgatctgcac gacagtgatg tcttgattgc catgctgcgc
     1081 ctgagcctgg ccgcccagga cagcgacgcg gtctcaccaa cggggcaggc aggccaatcc
     1141 aaacagcact ccgggaagac actggtgtcc tcggagcttg ccgcgcacat cttacaaccc
     1201 gacactgctc ccaccgtcga gcagcgctat ggattagagt tactcttgca gcgccaggag
     1261 caactgcgcg acgcccaata caccgcgttt cgccagcact ggtaccagct caaagcgcgc
     1321 gatttccgcc gcgaaatcat caccatttta gacaagtagc atcgtatatc ttatatgggg
     1381 cgcgcttcac cacgtcatgg tggcatatct atttagaaca gaaagcgggt aaaggacatg
     1441 atgaaaccat ttccggtgga ggaattggac tggtggcgta tgccgcagcc aacacaagag
     1501 gaactatggc tgagtggcgg cttgcgggtc gaagactggc cccatgcccg ccaggtgacg
     1561 cgcctctcga tccagttgtt tttcgccaca caacctctgc atgagctgga cgccctcgcg
     1621 ctgcgcctgc tcgaacgcgc cgccttcctg cacaacacgg gcatgttgat cgaggtgcgc
     1681 cgccatcata aacactcgtt cagcctgatt aaggaagccc cgctgcccga tttcagcgac
     1741 gacgaacgct acgagatcgc ctgcatcgcg cgctaccacc gccgcgccct gcccagcatg
     1801 aagcacgacg agtacgccgc gctcagtcgc gagtcgcgca agcgtgtcgc agcgctggcc
     1861 ggcctgctgc gcatcgcgga cgccctcgac tacaaccacg atgggcgcgt gctggagatc
     1921 gaggccgacc cgaaaggctg cgacaaagaa cactggacct tctctctgtg ggcgcgcccc
     1981 ctctccgacc tcgacctcga acttgaacac gcctacgaaa aagccgacct gttcgagaaa
     2041 gtatttcgcc gcaaagtgca gttcctggtg caagagtaat cactgtaatt acattgtcaa
     2101 ccatgacaga ccggtaggtg ccgattcatc gcgcacaccg ccgattcatc ggccacgacg
     2161 gaggtgagca gcaagcatca aacggatgat gagttttcag taccaggatc ggtcagggaa
     2221 tgtcatcctg agcgcagcga aggatctcag tagccgacgc atcagatcct tcgccgcgct
     2281 caggatgaca ttgccgatgg cagtcgtgaa acttcataat cggtacctac gaggttagca
     2341 tgtcgcgtgc aagcggttat ccctgttccc gcaacacaat cgacgcgccg caggcataat
     2401 cggggtgtat ccacagcgcg ggccgttcgc tctcaacgta ggttgacgtg acctcgtgcg
     2461 catccaggta actgcgcgcc ctctccatac tctccaccgc taatgtcatg cggcaaacgc
     2521 tttcgccgaa gcgagcaaga tggccgcgta atgcgcccgc gtccggcaaa tgctccaaac
     2581 caatctccgc gtcgtcctcg gctgccagtg ggatcggctc ggccagttcc aggctttgac
     2641 cgctatcgcc gagtgggaac gagaccagca tggcatccca gccatcggcc tcgcccgcaa
     2701 aggggataga tggttcgagt ccgtagatat gcgcgaaacg ccgcgcagct tcttgcaggt
     2761 tctcaacggc aatcgtggcg ctgagtacgc ccgtcgcgcc gagcgggtgc gccggtggcc
     2821 gcttccaacc ggccagccgc gaacggcgtt cctcgcccgc gctgtcgtgc tggatcagaa
     2881 aaggatagcg ctgcgccagg tctggccgct cgatttcgct gcgcagccag ccgcgctctc
     2941 ttccatcacc cgcgcgtaat tgtcccgccg tcggcccgat caatgccaca ccgcgccgcc
     3001 gtatcgcctc gctatcggaa gccaggtcat ccgatccgag tgccacattc agatagccct
     3061 cgcctttcgc caggcgctct accatgctcc gctgcgcctc ccccgccacg cgcaccgcga
     3121 tcaactccag gtacgtatcg ccaatcacct caacgcgatt ggccgttccc ccaaacggat
     3181 gaacgccgcc gccgctggcc tgcaagccca gctgctcact aaacctctgc gcggctgcat
     3241 caagatcatt gacgcccact atgatgtggt cgatgccgat aagcatagtt ttcctcgctc
     3301 gagtatccgg tgtcactaca atccgctcgt ctcacctggg tatacaaacc ccagggcgac
     3361 cgcaagggtc tccaccccac tccccaccgc tcccgccctt aca
//
LOCUS       DASZAK010001288         1546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_460616, whole genome shotgun sequence.
ACCESSION   DASZAK010001288 DASZAK010000000
VERSION     DASZAK010001288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460616"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1546)
                     /locus_tag="VGT44_22635"
     CDS             complement(<1..>1546)
                     /locus_tag="VGT44_22635"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEV2583675.1"
                     /translation="DIVYNLPTPGAPQNKPDFVVKMFGSTAFVVEDKSTDERLNEASI
                     EQLRRYTVGTSGYCLWTNARSVIGLRYDANGDHQTLVEVRVDEAFGSSQTAIRQDASF
                     EILHLLFHKKRFTEIASIIEEIAVDEQVWKQQAKSLSDETSLRAFITESRYVLDQLTT
                     AIQARLGNVTLELADAADDLTSSQQKYLTIVSSLFERLKGGGAVNLNALAILEGELVA
                     FASRLADVDTSYIERAKPAMAAATVPIWENAVQEMKGVISTLRERELARTESRRIRAA
                     YLLWLERYKFIEGEERGNDQDIEACRQQAFAEQVSYVFFVRLLLVRVLEDKGIMARLV
                     SDGGFKHWFSFLQTSTLEDRVHEIRGEAFLPLVYSRVVSYYRHFFQQPVFDWFLPDDY
                     LLALVLHRLNMYNFKDVTNDLLGFTYEAFIDRTARNQKGHFLTPPAIVEYMLDRAGYS
                     SSAVIGESLLDPACGSGSFLVHAARRLRTICFAKMAGSDDAQRARFFIEQVKTKLVGL
                     EVNPFSC"
BASE COUNT          332 a          437 c          439 g          338 t
ORIGIN      
        1 agcaggaaaa cgggttcact tcgagtccga ccagcttcgt ctttacctgc tcgatgaaaa
       61 accgggcgcg ctgggcgtcg tcgctgcctg ccatcttcgc aaagcatatc gttcgcaggc
      121 ggcgcgcggc atggaccagg aagctgcctg agccgcaggc ggggtcaagc aagctttcgc
      181 ctatgacggc agatgagcta tatccggcgc gatcgagcat atattcaaca atcgcgggcg
      241 gtgtcaggaa atggcctttc tggttgcgcg ccgtgcggtc aatgaaggct tcataggtga
      301 agccaagcag gtcgttggtc acgtccttga aattgtacat attcaggcga tgcagcacaa
      361 gcgccagcag gtaatcgtcg ggcaagaacc agtcgaacac gggctgctgg aagaaatgcc
      421 ggtagtagct gacgacgcgg ctatacacca ggggcagaaa cgcttctcca cgaatttcgt
      481 gaacgcggtc ttccaacgtg gaagtttgca ggaacgaaaa ccagtgctta aagccgccgt
      541 cggagacgag acgcgccatg atgcccttgt cctccaacac gcgcaccagc agcaggcgca
      601 caaagaagac gtaactgact tgctcggcaa aggcttgctg cctgcacgcc tcgatatcct
      661 gatcgtttcc gcgctcctct ccttcgatga atttatagcg ttccagccag agcaggtagg
      721 cggctcgaat gcggcgtgac tcggtgcgcg ccaactcgcg ctcgcgcagc gtcgaaatga
      781 cgcccttcat ctcctggacg gcgttctccc agattggcac ggtcgccgcc gccatggccg
      841 gtttcgcgcg ctcgatatac gaggtatcaa catccgccag gcgcgatgcg aacgcgacga
      901 gttctccttc gaggatcgca agagcgttga ggttgacggc tcctccacct ttcaagcgct
      961 cgaacaggct ggatacgatt gtgagatatt tttgctgact ggatgtcagg tcatcggcgg
     1021 cgtctgccag ttcaagcgta acattgccca gccgggcctg gatcgcggtc gtgagctggt
     1081 ccagcacata gcggctttcc gtgataaatg cgcgcaggga tgtctcgtcg gagagtgatt
     1141 tcgcctgctg tttccacacc tgttcgtcaa cagcaatttc ctcgataatg gaggctatct
     1201 cggtaaatct ctttttatga aagagcaggt gcagtatttc aaagctggcg tcctggcgaa
     1261 tggcagtttg cgacgacccg aatgcctcat caacacgcac ttccaccagc gtttgatgat
     1321 ctccgttcgc gtcgtagcgc aacccgatca ctgacctggc gtttgtccac aggcagtaac
     1381 ccgaagttcc aaccgtatag cgccgcaatt gctcgattga tgcctcgttc agtcgttcat
     1441 cggtgctttt atcctcaacc acgaaggcgg ttgagccgaa catcttcacg acaaagtcgg
     1501 gtttattctg cggcgcgccg ggcgttggaa gattatagac gatatc
//
LOCUS       DASZAK010001289         1819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_245335, whole genome shotgun sequence.
ACCESSION   DASZAK010001289 DASZAK010000000
VERSION     DASZAK010001289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245335"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="VGT44_22640"
     CDS             <1..751
                     /locus_tag="VGT44_22640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583676.1"
                     /translation="TSFGLAGTMFFLTQYLQLVLGYTPLEAGLRFIPLVLGLIAAAGL
                     SPQISPKVGVKVIISGGLVIVASGIALFALLNASSNYGQVVIALVVLGIGLGMAMVPA
                     TNAIMGAVPPGKVGVGSAVNDTSQEIGNALGVAILGSILAASYHSAINLAVLPVPDAV
                     KALAQDSIGGASVAAAHIGGPVGKAITSAANSAFIAGMNHTALLAAGISLCGALVALI
                     FLPSKRQDAAALHLTEESSLTTMKEEETLIP"
     gene            748..1344
                     /locus_tag="VGT44_22645"
     CDS             748..1344
                     /locus_tag="VGT44_22645"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2583677.1"
                     /translation="MTLSEQVKQKAGRPRSDRSHQAILKATLELLAIEGFETMSIEGI
                     AERAGVGKTTIYRRWSSKSDLVIEAIGKLHAELPVIDTGNLRNDLLAMFRNAWQQEGP
                     SIFESLGIHMIGILKTNPEISQAFFNRLFAPRLQQFTHMIERAKARGELQPDVDVVLL
                     VDLFAGPLIARLFFTSLISPPSEQWPEQVIDAILHGIA"
     gene            1477..1767
                     /locus_tag="VGT44_22650"
     CDS             1477..1767
                     /locus_tag="VGT44_22650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2583678.1"
                     /translation="MPISCRLRLLLARVNVERAIQGAPPLSLRRLSDESGVSLSVLAA
                     LNTGRSQRIDYNTIDHLLDYFNRYFHVTTGDLLAWEQRREGDSRFAFGQEVS"
BASE COUNT          372 a          488 c          542 g          417 t
ORIGIN      
        1 gacttcgttt gggctggcgg ggacgatgtt ttttttgacg caatacctgc aattggtact
       61 cggctacacg cctctcgaag ccggtctgcg tttcatcccc ctggtacttg gattaattgc
      121 tgccgccggg ttaagcccgc aaatttcgcc aaaagttggt gtgaaggtga tcatctctgg
      181 cggactggtg attgtggcta gtggcattgc gctcttcgct ttgctgaacg cgagcagcaa
      241 ctatggccag gttgtgattg cgcttgttgt gttggggatt ggcctgggca tggcaatggt
      301 tccggcgacc aacgccatca tgggtgcggt gccgccgggc aaagtgggtg taggctcggc
      361 ggtcaatgac acctcgcagg aaataggcaa cgcgctgggt gtggccatcc tcggcagcat
      421 cctggcagcc agctaccatt cggccatcaa tctcgcggtg ctgcccgtgc ctgatgcagt
      481 caaggcgctg gcacaggatt cgattggcgg cgcatcggtg gctgctgcgc acatcggcgg
      541 cccggttggg aaggccatca ccagcgcagc taatagcgca tttattgctg gcatgaacca
      601 tacggcgttg ctcgcagccg ggatatcgct gtgcggggcg ctggttgcgc tcatatttct
      661 gccatcgaag cggcaggatg cagcggcgct ccacttgacg gaagagagta gtctcactac
      721 aatgaaggag gaggagacgc tcattccatg actctatcgg agcaggtaaa acagaaggcc
      781 gggcgtccgc gcagcgaccg gtcgcaccag gctattctga aagccacgct tgaattgctg
      841 gctatcgaag gcttcgagac gatgagcatt gaaggcatcg cggagcgcgc cggggtcggc
      901 aagacgacta tctaccggcg ctggtcttcg aagtcggacc tggtgatcga ggccatcggc
      961 aagctacacg ctgaactgcc cgtgattgat acaggcaacc tgcgcaacga cctgctcgct
     1021 atgttccgca acgcctggca acaagagggt ccttcgatct tcgagagcct gggcattcac
     1081 atgattggca tattgaaaac caacccggaa atttctcagg cattcttcaa ccggttgttt
     1141 gctcctcgcc tgcaacaatt tacccacatg atcgagcgcg cgaaggcacg cggtgagctt
     1201 caaccggacg ttgatgtggt gctgctggtt gacctcttcg cggggccact catcgctcgc
     1261 ctgtttttca ccagcctgat ctcccctcct tcggagcagt ggccggaaca ggtcatcgat
     1321 gccattctgc acgggatcgc atgaccgcca ctcgcattgt tacgaaaaat cggcacattg
     1381 tattgacatc ccataacgta tttgctatag taaatagcat cgtaggaatg ctatgatgaa
     1441 tatgagaaga acattgttat cagggagggg gcgaccatgc ctatctcttg ccgactgcgc
     1501 ctgttgctcg ccagggtcaa tgtcgagcgc gctatacagg gcgcgcctcc tttgagcctg
     1561 cgccgcctct ctgacgagag cggtgtttct ttgagcgtac tggccgcgct caacacgggc
     1621 cgcagccagc gtattgatta caacacgata gaccacttgc ttgactattt caaccggtat
     1681 tttcatgtta ccaccggcga tttactggcc tgggagcaga ggcgcgaggg cgactcgcgg
     1741 tttgcctttg gccaggaagt atcatgaaat gaagcgctag cggagaattg agggaattac
     1801 tatggcggtt atgctgaag
//
LOCUS       DASZAK010001290         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_208711, whole genome shotgun sequence.
ACCESSION   DASZAK010001290 DASZAK010000000
VERSION     DASZAK010001290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208711"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VGT44_22655"
     CDS             complement(<1..502)
                     /locus_tag="VGT44_22655"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2583679.1"
                     /translation="MAKVIVPFNEQAEQFQISPDYVRISMAAAIQLGLKPGRMLRGAT
                     CGCINLLQNYPEGCYANCSYCGLARERPGNPEDNTFIRVAWPLYATDLVAEKIAEHAD
                     EVGRVCIAQVQDHRAYKDLVDMTRRVRRLSDVPISALVSATTLNVERLYRIQDAGADI
                     IGVGLDG"
     gene            complement(666..1694)
                     /locus_tag="VGT44_22660"
     CDS             complement(666..1694)
                     /locus_tag="VGT44_22660"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV2583680.1"
                     /translation="MSEAVFVTLDAMRAIPLPGSLEQAYAPDMQQVMDRAFALRQDHF
                     PDEITFHAPGLRRYKTAEYTGQDHRSFVSISLTGAECALACDHCNMSILKSMAALPRS
                     GGSLFEMCSELARQGASGVLISGGSDKKGRVPLLKHIPDLTRVRRELGMTIRVHPGIP
                     DEETVAALAEVGIDGAMMDIIGDNDTIRDVYHLADTTTENYERALALLDRYGVPSIPH
                     IILGLHYGQMRGEQRALEIIARHRHRAVVLVILMPLSSAPMAHVTPPPVEEITRFFAH
                     TRLALPQSPVMLGCARPAGHSKLPTDRAAVDAGFNGIAYPAEGIITYARQRGLQPKLI
                     NACCGVQW"
     gene            complement(1691..>2116)
                     /locus_tag="VGT44_22665"
     CDS             complement(1691..>2116)
                     /locus_tag="VGT44_22665"
                     /inference="COORDINATES: protein motif:HMM:NF021921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate protein ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEV2583681.1"
                     /translation="EAIEALEKSKYLASEWVHQTTAIPDTFGSAKIKTTVGLLDVRAT
                     LAGNTIKAIFIGGDFFAAEGAVADLEARLRWHPASPERVQETLAAAYAERATQLNNLP
                     LEALQEVIRQAVRDAQKAESAARADPYGCFVNPPEKVTS"
BASE COUNT          371 a          638 c          669 g          438 t
ORIGIN      
        1 cgccatccag ccccacgccg atgatgtcgg ctcctgcgtc ctggatgcgg tagaggcgct
       61 cgacattgag ggtcgtagcg gaaaccagcg cggagatcgg gacatcgctc aagcggcgca
      121 cgcggcgggt catatccacc aggtctttat aggcgcggtg atcctgcacc tgggcaatgc
      181 acacgcgccc aacctcgtcc gcgtgttcag cgattttctc cgcgaccagg tccgttgcgt
      241 acaggggcca cgccacgcgg ataaaggtgt tgtcttccgg gttgccgggg cgttcgcgcg
      301 ccaggccgca gtatgagcag ttcgcatagc agccttcggg ataattttgc aagaggttga
      361 tgcagccgca ggtcgcgccc cgcaacatgc gccccggctt caagccgagc tggatcgcgg
      421 cggccatcga gatacgcaca tagtccgggg aaatctggaa ttgctccgcc tgttcgttga
      481 aaggaacgat cacttttgcc atcggggttc tccttctcct ttcgtctgac ctgagtccgc
      541 agggctagcc gcgttatatc ttattccgac ctgcaacgca cttcgtgcta cgcttcgcgt
      601 cagccggtcg gagtggcttg cgcggcgttg gtcagacgga taagcgccga gtacgtttct
      661 acggactacc actgcacgcc gcagcacgca ttgatcagtt ttggttgcaa gccgcgctgc
      721 cgggcatagg taatgatgcc ctcggcggga taagcgatgc cgttgaatcc ggcatcaacc
      781 gccgcgcggt cggtcggtag tttgctgtgt ccggcagggc gagcgcagcc cagcatcacc
      841 ggggactgcg gcagcgccag gcgggtgtgg gcaaaaaacc gcgtaatttc ctcaaccggc
      901 ggcggggtga catgggccat cggcgcgctg gaaaggggca tcaaaatcac cagcacaacc
      961 gcccggtggc gatggcgcgc gatgatttct agcgctcgtt gttcgccgcg catctgcccg
     1021 tagtgcagcc ccaggatgat atgggggatg gacggcacac cgtaacgatc aagcaaagcg
     1081 agcgctcgct cgtagttttc ggtggtggta tcggcgaggt ggtacacatc gcggatggtg
     1141 tcgttgtcgc cgatgatatc catcatcgcg ccgtcgatgc caacttccgc cagcgccgct
     1201 acggtttcct cgtcggggat gcccgggtga acgcggatcg tcatgccgag ttcgcgccgg
     1261 acgcgcgtca aatcggggat atgcttgagg agcggcacgc gccctttttt atcactgccg
     1321 ccggagatca gcaccccgct cgcgccctgg cgcgccagtt cgctgcacat ctcgaacagc
     1381 gagccgccgg acctgggcaa tgcagccatg ctcttgagga tgctcatgtt gcagtggtcg
     1441 cacgccagcg cgcattctgc ccccgtcagg ctgatcgaca cgaagctccg gtgatcctgg
     1501 ccggtgtatt cggctgtttt atagcgcctc agaccgggag cgtgaaacgt aatctcgtct
     1561 ggaaaatggt cctggcgcag cgcgaacgct ctgtccatca cctgttgcat gtccggggcg
     1621 taggcttgct ccaggctgcc gggtagaggg atggcgcgca tcgcatccag cgtgacaaag
     1681 actgcttcgc tcatgatgtt accttctccg ggggattgac aaaacaacca tacggatcgg
     1741 cgcgagcggc ggattcggct ttttgggcat cgcgcactgc ctgcctgatt acctcttgca
     1801 aagcttccag ggggaggttg ttcaactgag tggcgcgttc tgcatacgct gcggccaggg
     1861 tttcctgcac tcgttccggg ctggcagggt gccagcgcag gcgcgcttcg agatcggcca
     1921 ctgcgccttc cgccgcgaaa aaatcaccgc caatgaagat ggctttgatc gtattgcccg
     1981 ccagggttgc gcgcacatcg agcagcccga cggtggtttt gatcttcgcc gagccgaagg
     2041 tgtcaggaat agccgtcgtc tggtgtaccc actcggacgc caggtacttg cttttttcca
     2101 gcgcttcgat ggcttc
//
LOCUS       DASZAK010001291         1515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_673308, whole genome shotgun sequence.
ACCESSION   DASZAK010001291 DASZAK010000000
VERSION     DASZAK010001291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673308"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1478)
                     /locus_tag="VGT44_22670"
     CDS             complement(<1..1478)
                     /locus_tag="VGT44_22670"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV2583682.1"
                     /translation="MQRALASHAWPNGVTVRARIGLYTGEPQLSAEGYVGLDVHHASR
                     IMSAGHGGQVLLSQTTRDLVEHNLPGGVSLQDLGAHRLKDLQHPSHLFQLVMVGLPAD
                     FPPPKTLDSHPNNLPIQPTSLIGREKEVATVQHLLRREDVRMLTLTGPGGTGKTRLGL
                     QVAAEASERFVDGVFFVNLAPISDPALVVPTIAQTLELKETGEQPLLDLLKTSLREKQ
                     LLLLLDNFEQVVSAASQVADLLAACPKLKIVVTSRMVLYVRAEHEFAVPPLALPDPTR
                     LPDLVALSQYDAIALFIQRAQAVKADFQVTNANAPAVAEICARLDGLPLAIELAAARS
                     KLFSPQALLPRLDSRLKLLVGGARDLPMRQQTLRGTIAWSYDLLEEAEKILFRRLSVF
                     VGGCTLEAAEAVCNSNGDLEIDVLDTIVRLVDKSLLRQEAEIDGEPRLLMLETIREYG
                     LEALAVNGETEDTRYAHAQYYMALAEEAEQGLDGPKQVMWLER"
BASE COUNT          305 a          454 c          455 g          301 t
ORIGIN      
        1 cgttccagcc acatgacttg ctttggccca tcgagcccct gttctgcctc ctcagccaat
       61 gccatgtagt attgagcatg agcgtatcgg gtgtcctcgg tctccccatt cacggcaaga
      121 gcctccagtc catattcgcg aatcgtctcg agcatcagta ggcgtggctc accatcgatc
      181 tcggcctctt gccggagcaa gctcttgtcc acgagtcgca ctattgtatc taacacgtcg
      241 atctccaggt ctccatttga attgcacaca gcctcggcag cttcaagcgt gcagcccccg
      301 acaaagaccg agagccgtct gaacagtatc ttctcggcct cctctaatag gtcataactc
      361 caggcgatgg tcccgcgcag cgtctgctgg cgcatcggca ggtcccgcgc tcctccgacc
      421 agcagcttga gccggctatc caaacgtggg agcagggcct ggggggagaa gagcttgctg
      481 cgcgctgccg ccaactcaat agcgagtggg agcccatcca agcgcgcgca aatctctgcc
      541 actgccgggg cgtttgcatt ggtcacctgg aaatcggcct tgaccgcctg ggctcgctgg
      601 atgaagaggg caatggcatc atactgcgaa agcgcgacga gatcaggcaa gcgtgtgggg
      661 tcaggcagtg ccagaggtgg gacagcaaac tcatgttcgg ctcgcacata gagcaccatt
      721 cggctcgtca ccacgatctt cagcttgggg caggcggcaa gcagatctgc cacctgcgag
      781 gcggcactga ccacctgctc gaagttgtcc aagagcagca agagctgctt ttctcgtagg
      841 gacgttttca gcaggtcaag caggggttgc tctccggtct ccttgagttc gagtgtctgg
      901 gcaatggtgg gcacgaccaa agcgggatca ctgatcggag ctaggttcac gaagaagact
      961 ccgtcaacga aacgttcact cgcttccgct gcgacctgca atcccagccg ggtcttgccc
     1021 gtgccaccag gaccggtcaa ggtgagcatc cgtacgtctt cacgacgcag caggtgctgg
     1081 acggtagcca cttccttctc gcgtccaatc aacgaggtgg gctggatggg caggttgttg
     1141 gggtgactgt cgagcgtctt ggggggcgga aaatcagcag gcaagcccac catgacgagc
     1201 tgaaagaggt ggctaggatg ctgtaagtcc ttgaggcgat gcgctcccag gtcctggaga
     1261 ctcactccgc ctgggaggtt atgctccacc aggtcgcggg tcgtctggga aagcagcacc
     1321 tgtcctccat gccctgcgct catgatgcgg gaagcgtgat ggacgtccag gcccacgtag
     1381 ccctcagcag aaagctgtgg ctcacctgta tagaggccga tacgggcacg cacggtcaca
     1441 ccattgggcc aggcatgact agcaagggca cgttgcatcc ctactgctgc tgagatagcc
     1501 tcggtggcgc gagca
//
LOCUS       DASZAK010001292         4309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_708512, whole genome shotgun sequence.
ACCESSION   DASZAK010001292 DASZAK010000000
VERSION     DASZAK010001292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708512"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VGT44_22675"
     CDS             complement(<1..520)
                     /locus_tag="VGT44_22675"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HEV2583683.1"
                     /translation="MIQFQYTLAQVHDREQWDSFVSACGHLLQSWEWGELKAGYGWYP
                     LRLALWDEGGNRIVAAAQALRRAPAHMPPRLGNLAYIPRGPMLGRSFETPGESGAGLL
                     YQAFYTQLLAALYNKGALAVQVELPQVMESEGGVQEQASPEASQVVHSLKAMGFHAIR
                     PVQPLRSILLDLT"
     gene            671..1081
                     /locus_tag="VGT44_22680"
     CDS             671..1081
                     /locus_tag="VGT44_22680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV2583684.1"
                     /translation="MNVVILGCGRVGAQLAKVMDQAGHSVSVIDSSSDSFQRLSSDFR
                     GETHIGNGVDEDVLTRAGIKRADVFVAVTNGDNRNIMASQIAKELFKVKKVVCRIYDP
                     IREETYRELGLETFCPTKLGAQMIFDMIIDRKQK"
     gene            1091..1753
                     /locus_tag="VGT44_22685"
     CDS             1091..1753
                     /locus_tag="VGT44_22685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV2583685.1"
                     /translation="MYIIIGGGGDIGYYLTKSLLSHGHEVLLIEKGSSRYGSLSEELG
                     TSVYRGDACEARTMDEVGASRADVMIAVTGDDEDNLVICQMAKKRFNVGRTIARLNNP
                     KHTEIFQMLGIDVTVSPTKAIVSLIESELPSSRFVQLMTLKNAGLEIVELRVQPGSPV
                     AGKVLRDVNLSRTSNITLILRGKEFIFPTADTQIIANDDIFALVTREGEEELRAAFGA
                     ND"
     gene            complement(1750..2949)
                     /locus_tag="VGT44_22690"
     CDS             complement(1750..2949)
                     /locus_tag="VGT44_22690"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV2583686.1"
                     /translation="MQQINWTKTRDILIVVICIGILLWAAMTVLGVVVHAVVLLLLAM
                     AAAFLVAPLVNFLDRYLPRVLAAVIVYVLILAGLGALCYALVFSLIQQVTYFSNNLPG
                     YVQNIPGTYTNTLDWLLKQGVPPSAIDKALSTISDQANAFVGSVATNLVSIVLIITNV
                     IVNVLLVTVMSFYLVVDGQRVRNALVGLSPKRAMKHVLIFEVALNRVVGNYIRGQLTL
                     AALIGIFAGLGCFFLGLSKFALIIGVLAFLFETIPMVGPALASIPAILISLLLPNPFP
                     RSYEILIYFVIVQMIESNILGPRILGHAVGLHPVASILALIIGAQLFGPFGALLATPV
                     LAACWVVIASLYSTLRGKPVENLLEKKRTPWAWRRKTSPLPMVVPVGGTEPDHEDAPE
                     QQDMLSV"
     gene            3233..3451
                     /locus_tag="VGT44_22695"
     CDS             3233..3451
                     /locus_tag="VGT44_22695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583687.1"
                     /translation="MDITALSTPTQMILAWVMISMLLGWFIVFAILAFHSNPDPNLDS
                     QELWDEQLTPARSFPTVTVQVGQSNTLR"
     gene            complement(3448..>4309)
                     /locus_tag="VGT44_22700"
     CDS             complement(3448..>4309)
                     /locus_tag="VGT44_22700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583688.1"
                     /translation="RVGNFMSDPRLQRARRSLHTPVSPSQQVRITEEEMIRDGVYEDR
                     KPGRLPSSVRRYPVSPDAGTEWSRRRADTYSDAPTRYTEYERTSIPPRRSATQTNLPA
                     VQAPPRRAPGTRGGQAPRSRSPDTDEQYDGYSSGLAVAPRRMRWHWLIFVGLSMFIMV
                     LGWLMFSSLNTWWTNTQNDWQYGRPRTYQVDAVVGHNDSKANPSHFIAINLNRQIIVI
                     EIPGGDASKTRIFNGPTLIGPGQDLTPVTLTFQDVNQDGKLDMVINVQDAHFVFLNEN
                     GTFKPAPGQT"
BASE COUNT         1016 a         1227 c         1151 g          915 t
ORIGIN      
        1 gggtgagatc gagcaggatg ctgcgtaatg gctgcacagg tcgaatcgca tgaaatccca
       61 ttgctttcag gctgtggacc acctggctcg cctccggtga tgcttgctcc tgtacgcctc
      121 cttcgctctc catcacctgc ggtaattcca cctgtacagc tagcgcgccc ttattgtaca
      181 gcgctgctag cagttgcgtg tagaatgcct ggtacagcaa gcctgccccc gactcgcccg
      241 gcgtttcaaa cgaccggccc agcattggtc ctctggggat ataggccagg ttcccaagcc
      301 ggggaggcat atgcgcggga gcgcgccgca gcgcctgggc agcagccaca atccgattgc
      361 caccctcatc ccacagcgcc agccgcaggg gataccagcc atacccggcc ttcaactcgc
      421 cccattccca gctttgcagc aaatggccgc aagctgagac gaagctgtcc cattgctccc
      481 tgtcgtgtac ctgggcaagc gtatactgga actgtatcac aatcctatct cttctcaata
      541 tttgaactta ttccatcacg cattatgata caatatcact cgatagtaac ggttgtagcg
      601 ctgcagctgc tggtgtccgc tggttgttcg gtcgccgcac gactgcgcat acaggaacag
      661 gaggcagttt gtgaatgttg taattctagg atgcgggcgt gttggcgcgc agctggcaaa
      721 agtcatggac caggcggggc attccgtttc ggtcatcgac tccagtagcg actccttcca
      781 acgcctgagt tccgattttc gtggagaaac ccacatcggc aacggcgtcg atgaagatgt
      841 ccttactcgc gccggcatca agcgagcaga tgtcttcgtc gctgtaacaa acggcgataa
      901 ccgcaacatc atggccagcc agatcgctaa agaactcttt aaggtcaaaa aagtggtgtg
      961 caggatctat gatcccatcc gcgaggaaac ctaccgcgag cttggattgg agaccttctg
     1021 ccctaccaaa ttgggcgcgc agatgatttt cgacatgatt atcgaccgca agcagaaata
     1081 agaggcagct atgtacatca ttattggagg tggcggcgat atcggctact acctgaccaa
     1141 aagcttgctc agccatggac acgaagtgct gctgatcgaa aagggaagca gccgctacgg
     1201 atcgctcagc gaggaattgg gaacatcagt gtatcgcgga gatgcttgtg aggcgcgcac
     1261 gatggatgaa gtcggagcaa gtcgcgccga tgtgatgatc gcggtaacag gcgacgacga
     1321 agacaacctg gtgatctgcc agatggccaa gaaacgcttt aatgtcggac gcaccatcgc
     1381 gcgcttgaac aatcccaaac ataccgagat ttttcagatg ctcggcatcg atgtcacagt
     1441 cagcccgacg aaagccatag tgtcgctgat cgaatcagaa ttgccgagtt ctcgctttgt
     1501 ccagttgatg acgctcaaaa acgcggggct ggagatcgtc gagttgcgtg tccagccggg
     1561 gtctccggtg gccggaaaag tcttgcggga tgtcaactta tcccgcacca gcaatatcac
     1621 cttgatcctg cgtggaaaag agtttatctt cccaacggct gacacacaaa ttatcgccaa
     1681 cgacgatatt ttcgccctgg tcacacgcga gggagaagag gagctgcgcg cggcgttcgg
     1741 cgcgaatgat taaaccgaca gcatatcctg ctgttccggc gcatcttcat gatctggttc
     1801 cgtgcctccc accggcacaa ccatcggcaa gggtgatgtt ttgcgccgcc aggcccaggg
     1861 agtgcgcttt ttctccagca gattttctac cggcttgccg cgcagtgtgc tgtacaggct
     1921 ggcaatcacc acccagcaag cggccagcac cggtgtggca agcagcgccc caaagggacc
     1981 gaagagctgc gctccgataa tcaatgccag aatcgaagcg acgggatgca gtccaaccgc
     2041 atgccccaga atacgcggcc ccaggatgtt gctctcgatc atctgtacga tcacgaagta
     2101 gatcaatatc tcgtatgagc gcggaaaggg gttgggcagg agcagggaaa tcagaatggc
     2161 ggggatcgag gccagcgccg ggccaaccat cgggatcgtc tcgaagagga aagccagcac
     2221 accgatgatg agcgcgaatt tgctcaatcc caggaaaaag catcccagcc cggcaaagat
     2281 gccgatcagt gccgccagag tcagttgacc gcgaatgtaa ttccccacta cgcggttcaa
     2341 ggccacctca aagatcagca cgtgcttcat ggcgcgtttc ggcgataggc cgaccagggc
     2401 attgcgcaca cgttggccat ccaccaccag gtaaaaactc atcaccgtca ccagcagcac
     2461 attcacgatc acattcgtaa taatcaagac gatgctgaca agattggttg ctaccgatcc
     2521 gacgaaggca ttggcctggt ccgaaatggt cgacaacgcc ttatcgatgg ccgacggcgg
     2581 cacaccctgc ttgagcagcc agtccagcgt gtttgtatac gttccaggga tgttctgcac
     2641 ataaccgggc aggttattcg agaaataggt cacttgctgg atcagcgaaa agaccagggc
     2701 ataacacaac gcccccagac ctgccaggat cagtacgtag acaatcaccg ctgccagcac
     2761 gcgagggagg tagcgatcca gaaagttgac cagcggcgcc accagaaacg ccgctgccat
     2821 cgccagcagc agcagcacaa ccgcgtgtac cacgacgccc agcacagtca ttgccgccca
     2881 tagcaagata ccgatacaga tgacaacaat caaaatgtcg cgcgttttgg tccagtttat
     2941 ctgttgcatt gagtctcgcc ccgtgtgaat ccgacgacgc attccgcgat catatatacc
     3001 ttttctccca ttgtggcagg tttgcgtcaa aagaacaaga gtcagtcgtt ctcattttgt
     3061 agaatgaacg gcgcgaacag agaagaatag cgcattcaac gcaacttttt ttcagtcacc
     3121 gtcgtgacgt atttacagcg cttcgccgta accaatcggc tagacgcagt taactttgct
     3181 gattttcttc gttcagtata gtaataatga cactcaaaac aaggatgtat tgatggatat
     3241 tactgctctc tcaactccca cccagatgat tcttgcctgg gtgatgatta gcatgttact
     3301 tggctggttc atcgtcttcg caatactggc ttttcactcc aaccccgacc cgaacctcga
     3361 cagtcaagag ctatgggacg agcagcttac gcccgccagg tcattcccta ccgtgaccgt
     3421 ccaggtggga caaagcaaca ccttacgcta agtttgccct ggcgcaggct taaacgtccc
     3481 attttcgttg agaaagacaa aatgggcatc ttgaacattg atcaccatgt ccagcttacc
     3541 atcctggttg acatcctgga acgtcagcgt aaccggggtt aaatcctgcc ctggcccgat
     3601 cagggtcggg ccgttgaaaa ttctggtttt agacgcgtct ccccccggta tttcaatcac
     3661 aatgatctgc cggttgaggt tgatcgcgat aaagtggctg ggattcgcct tcgagtcgtt
     3721 gtggccaacg accgcgtcaa cctggtaagt gcgcggtcgt ccatactgcc agtcgttctg
     3781 cgtgttcgtc caccaggtat tcaacgaact gaacatcagc cagccaagta ccatgataaa
     3841 catcgacagg ccgacaaaga tcaaccagtg ccagcgcatc ctgcgcggcg cgacagccag
     3901 gccgctgcta tagccatcat attgctcgtc ggtgtccggc gagcgagaac gtggagcctg
     3961 cccgccccgt gttcccggcg ctctgcgtgg tggggcctgc actgctggaa ggttcgtctg
     4021 ggtcgccgac cgccgaggtg gtatactcgt tcgttcgtac tcggtgtagc gcgttggcgc
     4081 atcgctataa gtatccgcgc gtcggcggct ccattccgta cccgcgtcgg gcgacacggg
     4141 atagcgacgc acactggaag gaagacgacc aggctttcga tcctcataca ccccatcgcg
     4201 tatcatttcc tcttccgtaa tgcggacctg ttgggaaggt gaaacagggg tatgaagcga
     4261 gcgcctggca cgctgtaaac gaggatcgct catgaaatta cccactctt
//
LOCUS       DASZAK010001293         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_316458, whole genome shotgun sequence.
ACCESSION   DASZAK010001293 DASZAK010000000
VERSION     DASZAK010001293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316458"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2389)
                     /locus_tag="VGT44_22705"
     CDS             complement(<1..2389)
                     /locus_tag="VGT44_22705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583689.1"
                     /translation="MIDSRTPDTSPALELNAERVRLAEAHENIKPWYYWGPYLSERQW
                     GTVREDYSSSGNAWDSFTHDHARSRTYRWGEDGLLGISDEQSRLCFAFSLWNEADPIL
                     KERLFGLTGSEGNHGEDVKEYYYFLDSTPTHSYMKALYKYPQRAFPYAELVAENRRRG
                     RGEPEYELIDTGVFEQDRYFDVIVEYAKVDPQDMLIRITATNRGPDAAPLHLLPTLWF
                     RNTWAWGYDDHDPEPRPELRVVDVPPALDGHARQRLVHANHATVGEYWLASQHDEQQP
                     QILFTENESNAQRLWGVPNRTPYVKDGINDAVVSGALEAVNPAGYGTKAAAHYTFVIA
                     PGSSETIMLRLSATQHAAPFDGAEEVFQSRQAEADSFYLTCGAKNLSEDARLVQRQAF
                     AGLLWGKQFYYYDVDMWLHGDPVGPPIPEAHKLGRNAEWGHLNTADIISMPDTWEYPW
                     FAAWDLAFHCIPLALVDPDFAKKQLILLLRVWYMHPNGQLPAYEWSFGDVNPPVHAWA
                     AWRVYKIEKRMTGKADRDFLERVFHKLLLNFTWWVNRKDSEGHNVFQGGFLGLDNIGV
                     FDRSAPLPTGGHLEQSDGTAWMGMYCLNMLMIALELARENRAYEDVATKFFEHFLYIA
                     DALNNIGGEGISLWDEEDQFFYDVLHMPDGSHVPLKVRSLVGFIPLLAVETIEPELLD
                     ALPDFKIRLEWFLEHRPHLAKLVSRWHEPGMEERRLLALVRGHRMKRLLKRMLDPEEF
                     LSNHGIRGISKYHADHPYVYYANGSAYSVRYEPGESSSALFGGNSNWRGPVWFPIN"
BASE COUNT          682 a          810 c          749 g          646 t
ORIGIN      
        1 aattgattgg gaaccagaca ggtccgcgcc agttggaatt gccgccgaat agtgcgctgc
       61 ttgactcccc aggttcatac ctgaccgagt acgcagaacc gttcgcgtaa tagacatagg
      121 gatgatcagc gtggtactta ctaatgcctc gaatgccgtg gttgctcagg aactcctctg
      181 gatcgagcat acgtttgagc aggcgcttca tgcgatgtcc acgcaccaac gcaagcaggc
      241 ggcgctcttc catccctggc tcatgccagc gcgaaacgag tttcgccagg tgcgggcgat
      301 gctccaggaa ccattccagg cgtattttga aatcgggcaa agcatccagc aactctggtt
      361 caatcgtctc caccgctaag aggggaataa acccgaccag tgagcgaact ttcaatggga
      421 catgcgagcc atctggcata tgcagcacgt cgtagaagaa ctgatcctcc tcgtcccaca
      481 acgagattcc ttctccccca atgttgttga gcgcgtccgc aatatacagg aagtgctcga
      541 agaacttggt ggcgacatcc tcatacgccc gattctcacg ggctaattcc agggcgatca
      601 tcagcatatt caagcaatac atccccatcc aggccgttcc atcggattgc tccaggtgtc
      661 ctcctgttgg taatggcgcg ctgcgatcga acacgccgat gttgtccaaa cccaggaacc
      721 cgccttgaaa gacgttgtgt ccctcgctat ccttgcgatt gacccaccac gtgaaattga
      781 gcaggagctt atggaagact cgctccagga agtcgcgatc agccttgccg gtcatgcgct
      841 tctcgatttt atagacgcgc caggcagccc aggcatgaac aggcgggttg acatctccga
      901 acgaccattc ataggccggt agctgaccat tgggatgcat ataccacacg cgcaggagca
      961 agatgagctg cttcttggcg aaatcggggt ccaccaaagc taatggaata cagtggaacg
     1021 ccaggtccca cgccgcgaac cagggatact cccaggtgtc gggcatcgag atgatgtcgg
     1081 cagtattgag atgcccccac tcagcgttgc gcccgagctt gtgtgcttct ggaatcggtg
     1141 gccctaccgg gtctccatgc agccacatat cgacatcgta atagtagaac tgcttgcccc
     1201 acagcagacc cgcaaacgcc tgccgctgca ctaagcgagc atcctcggac agatttttcg
     1261 caccgcatgt caggtaaaaa ctatcagctt ccgcctggcg tgactggaaa acctcttcgg
     1321 cgccgtcgaa aggagcagca tgttgtgtag cagacaatcg cagcatgatt gtctcgctcg
     1381 atcctggagc aatgacaaag gtgtagtgag ccgcggcctt ggtgccgtaa ccggcagggt
     1441 tcacagcttc caacgcaccg ctcaccacgg catcgttaat gccgtctttg acgtaaggcg
     1501 tacggttcgg cacgccccac aaacgctgtg cattgctttc attctcagtg aaaaggattt
     1561 gtggctgctg ttcgtcatgc tggcttgcaa gccagtattc acctacggtg gcatgatttg
     1621 catgcacgag acgctgcctt gcatgcccat ccagagcagg aggcacgtcc acgacacgca
     1681 gttccgggcg cggttctgga tcatgatcgt cgtatcccca tgcccaggta ttgcggaacc
     1741 acagcgtggg taacaggtgc agaggggctg catctggacc gcgattcgtc gcagtgatgc
     1801 gaatgagcat atcttgtgga tcaacttttg cgtactccac gatgacatca aaatagcgat
     1861 cctgctcaaa aacaccggta tcgatcagtt cgtattcagg ctcacctcgt ccgcgacggc
     1921 gattctccgc cacgagttca gcgtacggaa aggcacgttg cggatactta tagagagcct
     1981 tcatatagga atgggtgggg gtgctgtcaa ggaagtaata gtactccttc acatcctcgc
     2041 catggttccc ttctgagccg gtcaggccga aaagacgctc tttcaggata ggatccgcct
     2101 cgttccacag actgaacgcg aagcacaacc gggattgctc gtcgctgatg cccaggagac
     2161 catcttctcc ccagcggtac gttcgggagc gagcatggtc atgtgtgaag gagtcccagg
     2221 cattgccaga ggatgagtag tcctcgcgaa ccgtccccca ctggcgctcg gagagatacg
     2281 gtccccagta gtaccagggt tttatgttct catgcgcctc ggcaagcctg acgcgctctg
     2341 cattcagttc cagggctggc gatgtatcag gtgttctgct gtctatcatg tttatcgtct
     2401 tctcttcctt caaggtcata aggcaggaca ggttgggcag aggactaagc gctgtgttac
     2461 agtgccccga agttactgtc ttgcttgatg ccaattcgtt acatacagat gtgacattaa
     2521 gaccaaaagg cacaaaaatc cagatgacca aactaatccg atcagccgta gatgccgatt
     2581 tatcgtgcat accgccgatt catcggccct cggtgggctt tcacaatacc cctatgaaaa
     2641 cattcatctg catctttgtg cccgatatcg aaaatcccac aaaccttacg tatatctatc
     2701 atcaggttgt ctaatctggc tgatatgcat ataattgaga agagacaacc tctgctgcgt
     2761 tcagcacttg gttagaggag acaaacattg ggcatttcga acaacccatc actcacagat
     2821 caccagtacg cgggaacagt tgccgaccgg cgtgctaaag gcaagaatct acgtaaaaat
     2881 gtgcccc
//
LOCUS       DASZAK010001294         1636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_424186, whole genome shotgun sequence.
ACCESSION   DASZAK010001294 DASZAK010000000
VERSION     DASZAK010001294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424186"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VGT44_22710"
     CDS             <1..1312
                     /locus_tag="VGT44_22710"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583690.1"
                     /translation="LCKAQRGAVLLAEDHISPRTHEEDAQPAPVHSKALSTLALHNIQ
                     EEEASALLQAFPATTTLVHSPGMTCWITYRLDLNNVATDDADGTGNAPGVPFGDDSHA
                     EQGRHVLLVLGWVNESNEACAALVARCQALLPLVADAIGAALAIIDLRERMHDLERNA
                     VRESLQGMELLKAELLGTVSHELRSPLASVKGYAATLLRHERRLGREERHQFLLAINE
                     ASDRLEVIIERLLEVSQLETGQITINRSPVDLAHLAGEAIAAIAGRVEALFPGRFLFS
                     LRLTQADGAPAEAVPLLLADPRRLREVLDNLLENAVTYSPEGGAIDVTLRPVAQTQAG
                     APSRHSGEGGTGTPVPRDMLELCVSDSGQGIPVEHLQRIFDRFHRVDTRLTRETSGLG
                     LGLTICKRIVELHEGSIWAENRPQGNGCAFYVRLPLEREMARSY"
     gene            1340..>1636
                     /locus_tag="VGT44_22715"
     CDS             1340..>1636
                     /locus_tag="VGT44_22715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2583691.1"
                     /translation="MSAKRTTILTADDDPQLLRLMTRNLQLEGYDVLAASDGQQALEQ
                     IEQKSPDLVLLDVMMPRMDGFSVCHKVREFSSVPIIIVTARGQDQDKVRGLDLGA"
BASE COUNT          343 a          494 c          505 g          294 t
ORIGIN      
        1 cttatgtaaa gcgcagcgcg gcgcggtctt gctcgctgag gaccatataa gcccacggac
       61 gcacgaggaa gacgctcaac ctgctccggt tcatagtaaa gcattgagca cgctggcgtt
      121 gcataatatt caggaagaag aagcctctgc actcttgcag gcattccctg caacaaccac
      181 cctggtccac tctcccggta tgacctgctg gatcacttac cgactcgatt tgaacaacgt
      241 cgcgaccgat gatgctgacg gcacaggcaa cgcacccggg gtcccatttg gagatgattc
      301 tcacgccgaa caggggcggc acgtcttact ggtgttaggg tgggtgaatg aatccaatga
      361 ggcatgtgcg gcactggtgg cacgttgcca ggcactgctc ccactggtgg ccgatgcgat
      421 cggggcggcg cttgccatca tcgatctgag agaacgcatg catgacctcg agcgcaatgc
      481 ggtgcgcgaa tcgttgcagg gcatggaact gctcaaggcg gaattgctgg ggacggtcag
      541 ccacgaactg cgcagtcccc tggcctccgt caagggctat gccgccacgc tgctgcggca
      601 cgaacgccgc ctggggcgcg aggagcgtca ccagtttcta ctggccatca acgaggccag
      661 cgaccgcctg gaagtcatca tcgagcggct gctcgaggtc tcgcaattag agacgggcca
      721 gatcaccatc aaccgctcac cggtggacct ggcgcacctg gcgggcgaag ccatcgctgc
      781 gatagcgggg cgcgttgaag cgctcttccc agggcgtttc ctgttcagcc tacgcctgac
      841 acaggccgac ggcgcgcctg ccgaggccgt tcccctgctg ctggccgacc cgcgccgcct
      901 gcgcgaagta ctggacaatt tgttagagaa tgccgtgacc tattcaccgg agggtggagc
      961 catcgacgtc acccttcgtc cggtagcgca aacgcaagca ggagcgccat cccggcacag
     1021 tggtgagggg gggacaggga cgcccgttcc acgcgacatg ctggaactgt gcgtgtccga
     1081 tagcgggcag gggattccag tcgagcacct gcagcgcatc tttgaccgct tccatcgcgt
     1141 tgacacgcgc ctgacacgcg agacaagcgg acttggcctg gggctgacga tctgcaaacg
     1201 catcgttgaa ctgcatgagg ggagcatctg ggctgaaaac cgaccacagg gcaacggctg
     1261 tgccttctac gtgcggctcc ctctcgagcg agaaatggcc cggtcttatt aagtataaag
     1321 aaggaatgga gaacgaccga tgtctgcgaa gcgaacgacg atcctgactg ccgatgacga
     1381 cccacagctg ttacgactga tgacacgcaa cctccaatta gaaggctacg acgtcctcgc
     1441 ggccagcgat ggccagcagg cgctggagca gatcgagcaa aagtcgcccg acctggtgtt
     1501 actggacgtc atgatgccca ggatggacgg cttttccgtc tgtcacaaag tgcgcgagtt
     1561 ttcctcggta cccatcatca ttgtcacggc gcgcgggcaa gaccaggaca aggtacgcgg
     1621 ccttgacctc ggcgcg
//
LOCUS       DASZAK010001295         4418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_601959, whole genome shotgun sequence.
ACCESSION   DASZAK010001295 DASZAK010000000
VERSION     DASZAK010001295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601959"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1136)
                     /locus_tag="VGT44_22720"
     CDS             complement(159..1136)
                     /locus_tag="VGT44_22720"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEV2583692.1"
                     /translation="MRPIPTATPSAPPLQHIFYIMMENHGLNNILGNTADAPYLNQIA
                     NSYGIATRYFGVTHPSLPNYLAAISGSFQGIWDDCPAGASVTCAPQAFGGQLTGQQAN
                     QAASTAHLFAGQNLVDQLEAHGLTWKAYMQSLPATGFTGGYAGLYGQKHDPFMYFADI
                     RNSLARMQRIVPFTQFSADLQSGNIPNFAWITPDVCNDMHGASNCSGYDSLIAQGDAF
                     VRSAVSAIMSSPAWKAGAAIVIAWDESDASNDGCCNSPVGAAGAVLGGGNVPLIVLAS
                     RGPHHLVLGSQSYNHYSLLATIEQLWGLGCLANTCGISQAQTLTPLFEA"
     gene            complement(1443..1769)
                     /locus_tag="VGT44_22725"
     CDS             complement(1443..1769)
                     /locus_tag="VGT44_22725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583693.1"
                     /translation="MEITAAEHYDTIAHDLAFKDGDVALGKMFGMPCIKVNGKAFAGF
                     HRDEMVFKLTGSAHAQALGLDGARLFDPGMGHPMKEWVQVPFTNAEQWGTLAERALEY
                     VGKRGV"
     gene            complement(1783..3027)
                     /locus_tag="VGT44_22730"
     CDS             complement(1783..3027)
                     /locus_tag="VGT44_22730"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEV2583694.1"
                     /translation="MTSTNTTKSADTASRDWVALEHQYYQGTFKRQPVTFVRGKGTRV
                     WDSDGKVYLDLVAGIAVNVLGHCHPAIVEAVQQQVTQLVHVSNLYYNTRQIELAEQLG
                     LLSGGMRSFFSNSGAEANEGAIKLARKFGRVHKNGAFEVISMERSFHGRTLATTAATG
                     QAFYQATWVPLPDGFKQVPYNDLEALEAAVSDKTVAILLEAVQGEGGIWPGKQKYIQG
                     VRRLCDEQNLVMICDEIQAGMGRTGTFFSWEHYGVVPDIVTMAKGLAGGIPIGAMLSG
                     PRSDLFAAGDHGTTFGGNPIACAAGIATIQTILQENLIDNAAQMGKYCHQQFTHLCAQ
                     YPFIDSPRGIGLMQAVNVKHDLAPVIVQQALEHGLLLNALGKDTLRMVPPLNISKAEI
                     DEATHLLDLALADVAKMPVAEV"
     gene            complement(3068..4279)
                     /gene="argJ"
                     /locus_tag="VGT44_22735"
     CDS             complement(3068..4279)
                     /gene="argJ"
                     /locus_tag="VGT44_22735"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEV2583695.1"
                     /translation="MSDLFREVENGVTAPRGYRAGVTASGIKSDATVKDLAILASDVP
                     CVAAGTFTTSRTCAAPVIVCKERLKGGKAQAVIVNSGNANCATGETGLLNAYRMSEYA
                     ARLLGLENELVLCSSTGIIGRQLPIEKIEAGVKAIELSPDGGNAFSEAILTTDLRAKS
                     IALEFEIDGRFVRLGGATKGAGMIYPNMATMLCYLTTDAAIELDWMRIELRDAVNDSY
                     NMIAMDGDMSTNDTCVLFANGLAGNTPINAQHPQAGVFLEALRRVTQYLAREMARDGE
                     GATKLMTVHVRGARDKADAVKAARSVTLSPLWQCALAGGDPNWGRIVAALGASGCDIN
                     QETYDIFIGDVQVTQSGTAAHYSQEAARAEMAGNEVTVSVDLHLGDGQATAWGCDLTH
                     GYIDENTLYSR"
BASE COUNT          826 a         1321 c         1369 g          902 t
ORIGIN      
        1 ccgagggcga ccacaagggt gcgcccttac catgactggt cgaatggcaa cgcgagagcc
       61 gtttcgccgt catggtaagg gcgcaccctt gtggtcgccc tctgcaacgc ttgccctgcg
      121 cgtaaaaaaa tactttgcag tatgcaacgc atttgcaact acgcctcgaa caaaggcgtg
      181 agcgtctgcg cctggcttat gccgcaggta ttcgccaggc agcctaatcc ccagagctgc
      241 tcgatggtgg cgagcagtga ataatggttg tacgactggc ttcccagcac gagatgatgg
      301 ggaccgcgcg aagccagcac gatcaagggg acattgccgc ctcccagcac tgcgccggcg
      361 gcgccgaccg ggctattgca gcagccgtcg ttgctggcat cgctttcatc ccaggcgatc
      421 acaatggccg cgcccgcttt ccaggctggc gaggacatga tggcggagac agcgctgcgg
      481 acgaaggcgt cgccctgcgc gatcaggctg tcgtagccgg agcagttgga cgcgccgtgc
      541 atgtcgttgc acacatcggg cgtaatccag gcaaagttgg ggatgttgcc ggattgcagg
      601 tcggcggaga attgcgtgaa aggaacgatg cgctgcattc gcgcgaggct attgcggata
      661 tcggcaaaat acatgaacgg atcgtgcttt tgcccataca gtcccgcgta cccgccggtg
      721 aaacctgtcg cgggcaggga ttgcatatac gccttccagg tcagaccatg cgcttccagc
      781 tggtcgacca ggttctggcc ggcaaagagg tgcgcggtgc tggcggcctg gttcgcttgc
      841 tggccggtca gttggccgcc gaatgcctgg ggcgcgcagg tgacgcttgc cccggcggga
      901 caatcgtccc agatgccctg aaagctgccg gagatggcgg ccaggtagtt cggcaggctt
      961 ggatgcgtca cgccgaaata gcgcgtcgcg ataccatagc tgttggcgat ctggttcagg
     1021 taaggcgcgt cggcggtatt gccgaggata ttgttcagcc catgattctc catcatgatg
     1081 tagaagatat gctggagggg cggcgcggat ggcgtcgcag tagggattgg acgcacagtg
     1141 atttcaattt tgaaggtagc attgggagat agcaacggtg agccagtcgg cgagacccca
     1201 ggagcgttgc cgctgccaca gctggagacg aatagcagta gaatgcagcc cgctattagc
     1261 ctcctccatc gcgagatata tgtcctctcg tgtgacttca tcatcagatc ctctattgag
     1321 aggcaaacaa gcatcagaat ccctgtagga gcacggtaaa ccggctccta cagggatttg
     1381 gctctatttc tgcctggttg tttgcccact actattttct agaccccgct acatctctcg
     1441 ccctacacgc ctcgtttccc cacgtattcc agcgcgcgct cggccagtgt cccccattgc
     1501 tccgcgttgg tgaatggcac ctgcacccat tccttcatcg gatgtcccat gccaggatcg
     1561 aagaggcgcg cgccatccag tccgagggcc tgcgcgtggg cgctgccggt cagcttgaag
     1621 accatctcat cacgatgaaa acccgcgaaa gctttgccgt taaccttgat gcagggcatc
     1681 ccgaacattt tccccagcgc cacatcgcca tctttgaagg caagatcgtg ggcaatcgta
     1741 tcgtaatgtt ccgccgctgt aatctccata ccattcctcc aatcatacct ctgctacggg
     1801 cattttggct acatcggcca gggccaggtc gagcaggtgg gtggcttcgt ctatctcggc
     1861 tttggagatg ttgagcggcg gcaccatgcg cagggtatct ttgccgagcg cgttgaggag
     1921 caggccgtgt tcgagggctt gctgcacgat gaccggcgcg aggtcgtgtt tgacgttgac
     1981 cgcctgcatc aggccgatgc cgcgcgggct gtcgataaag ggatattgcg cgcacagatg
     2041 cgtgaattgc tggtggcagt acttgcccat ctgcgcggcg ttatcgatca gattctcctg
     2101 gagaatcgtc tggatggtgg cgataccggc ggcgcaggcg atggggttgc cgccgaaggt
     2161 cgtgccgtga tcgccagccg cgaagaggtc ggagcgcggc ccgctcagca tcgcgccgat
     2221 ggggatgccg ccggccagcc ccttggccat cgtgacgata tcggggacga cgccgtagtg
     2281 ttcccagcta aagaaggtac ccgtgcgacc cataccggcc tgaatctcat cgcagatcat
     2341 caccaggttc tgctcgtcac acaggcggcg cacaccctga atgtacttct gcttgccagg
     2401 ccagatgccg ccttcaccct gcacagcttc gagcaagatg gcgaccgtct tgtcgctgac
     2461 ggcggcttcg agcgcctcaa ggtcgttgta gggaacctgc ttgaagccgt cgggcagggg
     2521 cacccaggtg gcctggtaga acgcctggcc ggtagcggca gtcgtggcca gcgtgcgccc
     2581 gtggaagctg cgctccatgc tgattacctc aaatgcgccg ttcttatgga cgcgcccgaa
     2641 cttgcgcgcc agtttgatcg cgccctcgtt ggcctccgcg ccactgttcg agaagaacga
     2701 acgcataccg ccgctgagca gtccaagctg ctcggccagc tcgatctggc gcgtattgta
     2761 gtaaaggttg gagacgtgga cgagctgcgt cacctgctgc tgcaccgcct caacaatggc
     2821 gggatgacaa tgccccagca cattgacggc gatgccggcc accaggtcaa gatagacttt
     2881 gccgtcgcta tcccacacac gcgtcccttt gccgcgcaca aaagtgaccg gttggcgttt
     2941 gaacgtcccc tggtaatatt gatgctcaag cgcgacccag tcgcggctgg cagtatcggc
     3001 gcttttagta gtattggttg aggtcatcag caatgctcct ttgcaactca ttcacgcggg
     3061 cataaatcta acggctgtac aacgtattct cgtcgatgta gccgtgcgtc aggtcgcagc
     3121 cccaggcggt agcctggcca tcgccgaggt gcaaatcaac ggagacggtc acttcgttgc
     3181 ctgccatctc cgcgcgcgcg gcctcctggc tatagtgagc ggctgtaccg ctctgtgtca
     3241 cctgcacatc cccgatgaag atatcgtagg tctcctggtt gatatcgcag ccactggcgc
     3301 ccagcgcggc cacgatgcgg ccccagttgg gatcgcctcc cgcgagcgcg cactgccaga
     3361 gcggtgaaag cgtgacggag cgggcggctt tgacggcgtc ggccttgtcg cgcgcgccgc
     3421 gcacatgcac ggtcatcagc ttcgtcgctc cctcgccgtc gcgggccatc tcgcgcgcca
     3481 ggtattgcgt gacgcggcgc agggcctcga ggaagacgcc cgcctgggga tgctgcgcgt
     3541 tgatgggcgt gttgcccgcc agcccgttcg cgaagaggac gcaggtatcg ttggtggaca
     3601 tatcgccgtc catcgcgatc atgttatagg aatcgttgac ggcatcgcgt agttctattc
     3661 gcatccagtc gagttcgatg gcggcatcgg tggtcaggta gcacagcatg gtcgccatgt
     3721 tggggtagat catgcccgcg cctttggtcg cgccgcccag ccgcacgaag cggccatcga
     3781 tctcgaattc cagggcgata gatttggcgc gcaggtcggt cgtcaggatc gcttccgaga
     3841 aggcattgcc gccatcgggg gagagttcga tggccttgac acccgcctct attttttcga
     3901 tgggcaactg gcggccaatg atgccggtgg aagaacacag caccagttcg ttctccagcc
     3961 caagcagccg ggcggcgtat tcgctcatgc gataggcgtt gagcaggccg gtctcgcccg
     4021 tcgcgcagtt ggcgttgccg ctgttgacaa tcacggcctg agccttgccg cccttcaagc
     4081 gctccttgca gacgatgacg ggcgccgcgc aggtgcggct ggtggtgaag gttccggcgg
     4141 ccacgcaggg aacatctgat gctaaaatag ccaggtcttt cacggtggca tccgatttaa
     4201 tgccgctggc cgtaacgccc gcgcgatagc cgcgcggggc cgtgacgcca ttctcaactt
     4261 cgcgaaatag gtcagacaag caagtacctc ctccggggat aaactcttgt agccgacctc
     4321 attgtacaat ctggcaggcc gaaaccacaa ccccaaaggc agcaaacaac atcccggcat
     4381 ccgcgcggat gccgggatgt tgtttgctgc ctttgggg
//
LOCUS       DASZAK010001296         2397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_137860, whole genome shotgun sequence.
ACCESSION   DASZAK010001296 DASZAK010000000
VERSION     DASZAK010001296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137860"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VGT44_22740"
     CDS             <1..485
                     /locus_tag="VGT44_22740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583696.1"
                     /translation="TTGVTGVITFSRPKRSVRHPRWGVRYLWAYTVVFLTAIILSVQH
                     WPTDEYLVVLASIGYGFALGGYTARRFRLQPLVRRVVGKQWVVVHIAGMIGSYTVLWT
                     AFYVDNAHLIPGLKELPTLTFWVLPTVIALPFLLVSLSRFAPKILRYTRLDKASKGGI
                     "
     gene            488..940
                     /locus_tag="VGT44_22745"
     CDS             488..940
                     /locus_tag="VGT44_22745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583697.1"
                     /translation="MFLIALATAINGLLAGLNTDTALVKLPARRRIGAVAYATFARGN
                     DLGNGLVVYPLLGVGAALLTVLATALAYIVRSPIEVLLPLSIASLLSVLHSFATTRAA
                     PVMLSIKDAPDDEAILTAKLDRFAHWHAFRATFQVLAFFVLLWALVAM"
     gene            1014..1997
                     /locus_tag="VGT44_22750"
     CDS             1014..1997
                     /locus_tag="VGT44_22750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583698.1"
                     /translation="MREKVRQEASQSYPAGTTGTPAIELRALCKAFGPLQAIDHLTLA
                     VEQGEIFGLLGPNGSGKTTTINLISGLSTPTSGEVRVMGYEVHRYARRIRQMLGAVPQ
                     ETALYEELSAWDNLDFHADLFGMARKEKKERITPMLELVQLQERKHSRVGTFSGGMKR
                     RLALARALLHDPHLLYLDEPTLGVDVQSRRAIWDYILALHKEGKTVLITTNYLEEAQA
                     LCERIAILDHGKLIAVDTPQHLKQTYGGSVVELETAQPSAVADDIRRLRGVKEVIQEG
                     THMQITTQAGSLVVPQIINLVTGQGELRQIAVREPNLDEIFLRLTGTALRD"
     gene            2010..>2397
                     /locus_tag="VGT44_22755"
     CDS             2010..>2397
                     /locus_tag="VGT44_22755"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583699.1"
                     /translation="MHTLDSSLRVIWACLKKDLKSARSEPLFTLVSIILPLNVLFLMS
                     LLVISGGLAPTAVVMQDSGPLAQQFYAAMSDAHSFVLQQATAEQANALIEQGKIVAVV
                     TIPADFDTRIRLHQSIAVGVKINNLNT"
BASE COUNT          497 a          697 c          686 g          517 t
ORIGIN      
        1 tgacgacagg agtgactggc gtgatcacgt tcagcaggcc caaacggagc gttcgccatc
       61 caagatgggg agtacgctat ctgtgggcct acaccgtggt gtttctcacg gccatcatcc
      121 tgtcggtcca gcactggcca acggacgagt acctcgtcgt tctagcctcg atcggctatg
      181 gttttgctct cggaggctat accgcgcgac gctttcgact gcaaccactg gttcggcgcg
      241 tcgtggggaa gcagtgggtc gttgtccata ttgccggcat gattgggtcc tacaccgtcc
      301 tgtggacggc cttttatgtg gataacgccc acctgatccc aggcttgaag gagcttccga
      361 cactcacgtt ctgggtgctg ccaaccgtga tcgcgctccc gttcctgctg gtgtccctct
      421 cccgttttgc gccgaaaatt ctcaggtaca cgcgactcga taaagcatcg aaaggaggca
      481 tatagttgtg tttctgattg cgcttgcaac agcgatcaat gggctgctgg caggcctcaa
      541 tacggatacc gcgcttgtga agttacctgc gcgcaggcgc attggtgccg tggcgtatgc
      601 tacctttgct cgtggcaatg atctagggaa cgggctggtg gtctatccgc tgctgggtgt
      661 cggcgcagcc ctgcttaccg tgcttgccac ggctctggcc tacatagtgc gctccccaat
      721 agaggtattg ctgccgcttt ccattgcctc actgctgagt gtccttcata gcttcgccac
      781 caccagagct gcgccagtga tgctcagcat caaggacgca cccgatgacg aagccatctt
      841 gaccgcgaag ctggaccgtt ttgcacactg gcatgctttt cgggcgacat tccaggtgct
      901 ggcgtttttc gttctgctgt gggcgttagt agcgatgtga cactgatcag cccacgatct
      961 gtgagcaaag acattttttg ttctggttgc tcttaacaga aagagagaga cctatgcgtg
     1021 aaaaagtgag gcaagaagca tcacaatcct atccagctgg aaccacaggg actcctgcta
     1081 tcgagctgcg agcactctgc aaagcattcg gacccctgca agccattgac cacctcaccc
     1141 tcgcggtcga gcagggagag atctttggcc tgctgggacc caacggatca ggtaagacca
     1201 ccactatcaa tctgatcagt ggcctctcca ccccgaccag cggcgaggtg cgggtgatgg
     1261 gctacgaggt acatcgttat gcgcgtagga tacggcagat gctgggtgcc gtgccccagg
     1321 agaccgcgct ctacgaggaa ctctcagcct gggacaacct ggactttcac gcggatctct
     1381 ttggcatggc acgcaaggaa aaaaaggaaa gaatcacgcc gatgctcgaa ctggtccaac
     1441 tgcaggaacg caagcattcg cgtgtgggca ccttctccgg tgggatgaag cgcaggctcg
     1501 cactggcgcg ggcgctgctg cacgatccgc atctgctcta cctcgatgag cctaccctgg
     1561 gcgtggatgt gcaatcgcgc cgcgccattt gggattatat cctcgcgctg cacaaagagg
     1621 gtaagactgt cctgatcact accaattacc tggaagaggc gcaggccctc tgcgagcgca
     1681 tcgctattct cgatcatggc aagctcattg ctgttgatac cccgcagcac ctcaaacaga
     1741 cctacggtgg tagcgtcgtg gaacttgaaa cggcgcagcc aagcgcggtc gccgacgaca
     1801 tccgccgact cagaggcgtc aaggaggtca tccaggaggg aacgcacatg cagatcacca
     1861 cgcaggcagg aagcctcgtc gtaccccaga tcatcaacct tgtcaccggg cagggagaac
     1921 tgcgccagat cgccgtgcgc gaacccaacc tggatgagat tttcctgcgt ttgacgggaa
     1981 cggcgctacg agattaggag gcaagcatca tgcacacact cgactcttcc ctgcgtgtca
     2041 tctgggcctg tctgaaaaag gacctcaaaa gcgcgcgctc cgagccactt tttaccctgg
     2101 tttcgatcat tctcccgctg aatgtgctgt tcctgatgag cctgctggtg atcagcggag
     2161 gactggctcc cacagcggtg gtcatgcagg attccggtcc cctggcgcaa cagttctacg
     2221 cggccatgag tgatgcccat tcgtttgtgt tacagcaagc caccgcggag caggccaatg
     2281 cgttgatcga gcaaggcaag atcgtggcgg tcgtcacgat cccggcagac tttgatacgc
     2341 gcatccgcct gcatcagagt attgcggtgg gggtgaagat caacaacctc aataccg
//
LOCUS       DASZAK010001297         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_105342, whole genome shotgun sequence.
ACCESSION   DASZAK010001297 DASZAK010000000
VERSION     DASZAK010001297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105342"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VGT44_22760"
     CDS             <1..208
                     /locus_tag="VGT44_22760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583700.1"
                     /translation="GHLRAHSVSYDRRDEFFGEFDHSAILDLLADQNETAALAATTGL
                     LRSYAIDVAPETLASELQESGAGR"
     gene            205..1023
                     /gene="cobA"
                     /locus_tag="VGT44_22765"
     CDS             205..1023
                     /gene="cobA"
                     /locus_tag="VGT44_22765"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEV2583701.1"
                     /translation="MIDSLVTPIDPASITAGNPPGKVYLIGAGPGDPELITVKGLRCL
                     SRADVVLYDHLVSPELLAEAPARAELVSVGKRAGHHSMAQAEINALMISYACAGRVVA
                     RLKGGDPFVFGRGGEEVLALIQAGVPFEVVPGISSALAVPASAGIPVTHRDYASSVTI
                     VTGHPADKGEVVNWKALAQLGGTLIVLMGVKSLGDFTCQLREAGLSPDLPAAVIQEGT
                     TPRQRVVVGTLADIATRAQAAGLASPATTVIGHVVRTFSASGDLRRASPCGRPP"
     gene            complement(1058..1975)
                     /locus_tag="VGT44_22770"
     CDS             complement(1058..1975)
                     /locus_tag="VGT44_22770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV2583702.1"
                     /translation="MAVIEKVPVAAVGAQARPRARSRSHWTWQSLTTVFMAIMLIYFV
                     LPLFWLIASATKTNPELFSTFGLWFAPDFNLWNNLVGVFTYDGGIFLTWLGNSIYYAV
                     CSAIGASLIATLAGYVFAKFRFPGRTLIFAIILGSIMVPNTALAIPLYLLLSKVSLID
                     TPLAIILPSLVTPFGVFLMRVYSEQAIPDDLLDAARVDGAGELRIFWSIALRILSPGF
                     VTILLLTFVGTWNNYFLPLLVTNNPNLYPLTVGLANWNQLAQSFSGSQILYNLVITGS
                     LISILPLIVGFLFLQRYWQSGLTFGSVKS"
     gene            complement(1975..2637)
                     /locus_tag="VGT44_22775"
     CDS             complement(1975..2637)
                     /locus_tag="VGT44_22775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV2583703.1"
                     /translation="MLKDRFHRAMLGLALMLALLLDSKIARLKTLFRLGYFLPYAIPS
                     VIGALLWGYLYSQNIGPFAQLATALHWPPPGFLTLAGVLPSIGNIVTWQWTGYNMIIM
                     YAALQAVPAELYDAALVDGASGWDIARYIKIPLIVPAIIVTIVFSIIGTLQLFNEPQI
                     LSAIIPNIIQDHFTPNLYAYNLAFTNQQYNYAAAVSFVLGAVVFVFSYVFMLATNRRG
                     LR"
     gene            2617..2868
                     /locus_tag="VGT44_22780"
     CDS             2617..2868
                     /locus_tag="VGT44_22780"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEV2583704.1"
                     /translation="MEAVLEQGLRVPQDISIIGLDDIVISAHLRPPLTTIAVPKSQLA
                     QEATELLLAQIAGEEELHIARLVEPYLVVRQSTTSPLQE"
BASE COUNT          697 a          907 c          905 g          569 t
ORIGIN      
        1 cggtcattta cgcgcgcata gcgtatccta cgaccggcgc gacgagttct tcggcgagtt
       61 tgaccattcc gctatcctcg atctcctcgc ggatcagaac gaaacggcgg cgctggccgc
      121 caccacgggc ttgctgcgca gctatgctat cgacgttgcg cccgaaacgc tggcaagcga
      181 actgcaagag agtggagcag gccgatgatc gattcactcg tgacccctat cgatccggca
      241 agtatcaccg ccggcaaccc tcctggcaag gtgtacctga tcggcgctgg ccccggcgac
      301 cccgaattga ttaccgtcaa aggattgcgt tgcctgagca gggccgacgt ggtgctgtac
      361 gaccacctgg ttagcccgga attgctcgct gaagcgcctg cgcgcgccga gctggtatcg
      421 gtgggcaaaa gagcgggaca ccacagcatg gcccaggccg aaatcaacgc gctgatgatc
      481 tcctacgcct gcgcaggtcg cgtggtcgcg cggctcaaag ggggcgatcc attcgtcttt
      541 gggcgcggcg gcgaagaagt tttagccttg attcaggcag gagtgccgtt cgaggttgtg
      601 ccgggcatca gttccgcgct cgccgtgcca gcctcggcgg gcattcccgt gacgcatcgc
      661 gattatgcct cctccgtcac aatcgtgacc ggccacccgg cggacaaggg cgaggtggtc
      721 aactggaaag ccctggcgca gctgggcgga acgctcatcg tgctgatggg cgtgaaatcg
      781 ctcggcgact tcacgtgcca actccgggaa gccggactct cgcccgacct gcctgcggcg
      841 gtcatccagg aaggcacaac gccccggcag cgcgtcgtgg tcggcacgct cgccgacatc
      901 gccacgcgcg cgcaggccgc cggtctggcc tccccggcaa cgacggtgat tggacacgtc
      961 gttcggacgt tttctgcatc aggagattta cggagggcgt ccccttgcgg gcgccctccg
     1021 tgaaacctac cctccctatc gcgggtacta tcatttccta cgatttcaca ctgccgaacg
     1081 tcagcccgct ctgccagtag cgttgcagga acaggaaccc tacgatcagg ggaagaatgg
     1141 aaataagcga gccggtgatg accaggttat acagaatctg cgacccactg aacgactggg
     1201 cgagctggtt ccagttggcc agtcccaccg tcagcgggta gagattggga ttgtttgtga
     1261 ccagcaaggg caggaagtag ttattccagg tgccgacaaa ggtcagcagc aggatcgtca
     1321 caaagcccgg cgacaggatg cgcagcgcga tgctccagaa aatgcgcagt tctcccgctc
     1381 catcgacccg cgcggcgtcc agcagatcat ccgggatggc ctgttccgaa tagacgcgca
     1441 tcagaaacac tccgaatggc gtcaccagcg acggcaggat gatggccagc ggcgtatcaa
     1501 tcaggcttac tttgctgagc agcaaataga gcgggatggc cagggcggta ttgggaacca
     1561 taatcgaccc cagtatgatg gcaaagatca gggtacggcc aggaaagcga aacttggcaa
     1621 agacataccc cgccagtgtc gcgatcagag aagccccgat tgcgctgcac acagcgtagt
     1681 agattgaatt gccgagccag gtcagaaaaa tgccgccatc gtaggtgaac acgccgacca
     1741 agttattcca gaggttgaag tcgggcgcga accacaggcc aaaggtggaa aatagttccg
     1801 ggttggtttt ggtcgccgag gcgatcaacc agaacagcgg cagcacgaag tagatcagca
     1861 tgatcgccat aaagaccgtc gtcaggctct gccatgtcca gtggctcctg ctgcgtgcgc
     1921 ggggccgcgc ctgcgcgccg accgcagcga ccgggacttt ctctattact gccatcagcg
     1981 taaccccctg cgattcgttg cgagcatgaa cacatacgaa aagacaaaca caacagcgcc
     2041 cagcacgaac gaaaccgccg ccgcgtagtt gtactgctgg ttcgtgaatg ccaggttata
     2101 ggcatacagg ttgggcgtaa agtggtcttg aataatattc gggataatgg ccgacaagat
     2161 ttgcggctca ttgaacaact ggagcgtacc gatgatggag aacacgatgg tcacgatgat
     2221 ggccggcacg atgagcggta ttttgatgta gcgcgcgata tcccagccgc ttgctccatc
     2281 caccagcgcg gcgtcgtaga gttccgccgg caccgcctgg agtgcggcat acattataat
     2341 catgttgtag cctgtccact gccacgtcac gatattgcca atcgatggca ggacgcccgc
     2401 gagcgtgaga aagcccggcg gcggccagtg cagcgccgtc gccagttgcg caaatggccc
     2461 gatgttctgg ctatagagat aaccccacag taacgcaccg atcacgctcg gaatcgcgta
     2521 gggcagaaaa tagcccaggc ggaagagggt tttcagacgc gctattttac tatcgagcaa
     2581 cagcgccagc atcagggcaa gccccagcat ggcgcgatgg aagcggtcct tgagcaaggg
     2641 ctgcgtgttc cccaggatat ttctatcatc ggcctggacg acatcgtcat atcggcccac
     2701 ctgcgccctc cactgacaac cattgcggta ccaaaatccc aactggcgca agaagcaacc
     2761 gaactgctgc ttgcccagat cgctggagag gaggaactac acatcgcgcg ccttgttgag
     2821 ccgtatcttg tagtgcgcca atccaccacc tcgcctctgc aagaatgaat ctttgcaaaa
     2881 gcgcccctga tgaatagaat aaatcgtgta tagtttaccg tacatcagca attccgtatg
     2941 acagtacatc gcctggcagt atcgtatgat gaggaggacg caccacatgc ctgctgacca
     3001 tcatctccac gacaccaccc cgaatgttgt gtagagatgg cggtcaatca tcagagagca
     3061 gtgaagctgg ctgatacc
//
LOCUS       DASZAK010001298         5686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_138019, whole genome shotgun sequence.
ACCESSION   DASZAK010001298 DASZAK010000000
VERSION     DASZAK010001298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138019"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /gene="ilvN"
                     /locus_tag="VGT44_22785"
     CDS             complement(<1..404)
                     /gene="ilvN"
                     /locus_tag="VGT44_22785"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV2583705.1"
                     /translation="MTSQEAGQSGRSGQSGAPEGEVQAHTLVMLVDERHGALDRIVGL
                     LRRRRARPRKIAISQSETPGVARISVVMDDSEVEIEHLIEQMRKIVDVRQIINMSAQQ
                     SISRELALVKVNNSAARNSEIIELGHLFGAHVV"
     gene            complement(421..2220)
                     /gene="ilvB"
                     /locus_tag="VGT44_22790"
     CDS             complement(421..2220)
                     /gene="ilvB"
                     /locus_tag="VGT44_22790"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEV2583706.1"
                     /translation="MQVAATITRQESGTTMSPQHNVERPQQESDARELAGSQIMCEAL
                     IREGVELLYGYPGGAIMPFYDSLTAYPTLHHVLTRHEQAAAHAADGYARATGKVGVCV
                     ATSGPGATNLVTGLATAYMDSTPIVAITGQVARQFIGRDAFQETDVLGLTQPITKHNF
                     LVQRVEDLADIVHEAFRLARDGRPGPVLIDVPKDIQFAKTMYRPSASICSVDYFRAHR
                     PTQPAPEAIVQAARLIGEAQRPVIMAGHGVIFSRAYDELRAFAEKTNIPVITTLLGLS
                     AFPETHPLSIGMPGMHGPAHVNRAIGETDLIIGVGLRFDDRVTGKVSEFAPRARIIHI
                     DIDPSEMHKVKRAAVPIVADAKAALAALVEATQPGDHRAWINEVRDWEAQNNERIAAM
                     PRGDDSTASPDPITILQAIRAETKGEAMIVSDVGQHQMWTARHYTYTRPNSHITSGGL
                     GTMGFALPAAMGVKMGMPESVVWAIAGDGGIQMNIQELATLRQEGVTVKVAIMNNGYL
                     GMVRQWQQFFHNRNYSETPITGPDYTKLAEAYGLKGMRVTRRADVAAAIHEAMETEGS
                     VIIDFVIEPESNVYPMVAPGAAITNMIQEQVEQ"
     gene            complement(2352..2660)
                     /locus_tag="VGT44_22795"
     CDS             complement(2352..2660)
                     /locus_tag="VGT44_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583707.1"
                     /translation="MLATWTTINRSAMAADRMDGERMPSCQQQAPMSHAGMTGMATQA
                     TPSMIETSVLALVSLFAPVVFILRGLLLVALLALSGLTGLVSLVGLTTELDASALQGD
                     "
     gene            complement(2862..3062)
                     /locus_tag="VGT44_22800"
     CDS             complement(2862..3062)
                     /locus_tag="VGT44_22800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2583708.1"
                     /translation="MAERSDILLTVREVATRLRVDQTTVRRWIKDGALEAVSLPHRGK
                     REVYRVRQSTIDRIIESRQLPK"
     gene            3359..4450
                     /locus_tag="VGT44_22805"
     CDS             3359..4450
                     /locus_tag="VGT44_22805"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEV2583709.1"
                     /translation="MKKAEDAIPNELLSFEREQRLWTQEEVAEHIGAPDANMVGRWER
                     GITRPTSYYRQQLLTLFGKSTRELGFVRKGEIPFWHVPYRQNAYFTGRDELLRQIHTH
                     FKQQQRTRPLPPLALSGLGGVGKTQTALEYAYRYRHEYHTVVWVRAESSEALISDFIA
                     FAGLLNIPDCQQQDHQSTINAVKYWLSAMTRWLFIFDNVDDLAMLNDFLPSAPRGHLL
                     LTTRMQTTGTAAETLAIESMNPMEGAYFLLRRSKIVGFNAAFDDIGKDDHHLAVSISE
                     KLGGLPLALDQAGAYIEETACGLDGYLERYQQLREAFLQRRGHADLHHPEPVATTWSL
                     AFEKASQSNIDSIALLRFLAFFSPDAIPL"
     gene            4658..5629
                     /locus_tag="VGT44_22810"
     CDS             4658..5629
                     /locus_tag="VGT44_22810"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2583710.1"
                     /translation="MLVRAINATFPSGEFGTWQQCERLLSQALRGVELANQWNTISPE
                     AARLTHHVANYLRNRGHFALAEQLYQQALAQYEAAGQSELREVASCLYQMGDLYLTLK
                     DYDKTFAFFQRAVAIQEEAPGDMRPDLARTLDTIGEAYYYLEDYAPAEDYFRRALVLR
                     QDIFGSDHPDTAESLNNIGILLLKCLRRYDEAEIVFQQVLAILTKHRGPDHKDVGICM
                     LNLATTYREQGKYEVGKQWYLRTIALWRRILGEEDTDVAFALRALAKLYALEGSEQAE
                     ATYLQALAIYEKARGPEHRVTLEVKRELEELRQAPFRHLASFKGAGE"
BASE COUNT         1195 a         1681 c         1573 g         1237 t
ORIGIN      
        1 acaacatgcg cgccgaacag gtggccgagt tcgatgattt cactgttgcg cgccgccgaa
       61 ttattgacct tcaccagcgc cagctcgcgc gagatggact gttgcgcgga catattgatg
      121 atctgccgca catcgacgat cttacgcatc tgctcgatca ggtgctcaat ctccacctcg
      181 gaatcgtcca tcacgaccga gatacgcgcc acgccgggcg tctcgctctg gctaatggcg
      241 attttgcgcg gcctggcgcg gcggcggcgc agcaggccca cgatgcggtc gagcgcgccg
      301 tgccgctcgt ccaccagcat caccagcgtg tgcgcctgca cctcgccttc gggagcgccc
      361 gactggcctg atctcccgct ttgccccgct tcttgactgg tcatggttcg catatgcctc
      421 ctactgctcc acttgttcct gaatcatatt ggtgatggcc gcgccggggg caaccatcgg
      481 gtagacgttc gactccggct cgatcacgaa atcgataatc acgctgccct cggtctccat
      541 cgcctcgtga atggctgccg cgacatccgc gcgccgcgtc acgcgcatcc ccttcaggcc
      601 ataggcttct gccagtttgg tataatccgg gccagtgatc ggcgtctcag agtagttgcg
      661 gttgtggaag aattgctgcc actggcgtac catgcccagg tagccattgt tcattatggc
      721 gaccttgacg gtcacgcctt cctgccgcaa ggtggccagt tcctggatgt tcatttgaat
      781 accgccgtcg cccgcgatgg cccacaccac gctctccggc atccccatct tgacgcccat
      841 cgccgccggg agggcaaagc ccatcgtgcc tagcccgccg gaggtgatat ggctgttggg
      901 gcgcgtgtag gtatagtggc gcgccgtcca catctggtgc tgccccacgt cgctgacgat
      961 catggcctcg cccttcgtct ctgcgcgaat ggcttgcagg atggtgatgg gatcgggaga
     1021 cgccgtgctg tcatcgccgc gcggcatagc ggcgatgcgt tcgttgttct gcgcctccca
     1081 gtcgcgcacc tcgttgatcc aggcgcggtg atcgcccggc tgcgtggcct cgaccagcgc
     1141 tgccagagcc gccttcgcgt cggccacgat gggaacggcg gcgcgcttga ccttgtgcat
     1201 ttcggatggg tcgatgtcga tatggatgat gcgtgcgcgc ggcgcgaact cgctcacctt
     1261 gccggtgacg cgatcatcga agcgcagccc gacgccaata atcaggtcgg tctcgccgat
     1321 ggcgcgattg acgtgcgccg ggccatgcat ccccggcata ccgatggaaa gtgggtgcgt
     1381 ctccgggaaa gcgctcaggc cgagcagcgt ggtgataacc ggaatattgg tcttctcggc
     1441 aaaggcgcgt agttcgtcgt aggccctgga gaagatcacg ccatgcccgg ccataatcac
     1501 cgggcgctgt gcttcgccga tgagccgcgc cgcctggacg atggcctccg gcgctggctg
     1561 cgtcgggcga tgcgcgcgga agtaatcgac cgagcagatc gaggccgacg ggcgatacat
     1621 ggttttggcg aactggatat ctttgggaac gtcgatcagc accggccccg gtcggccatc
     1681 gcgcgccagc cggaacgcct cgtggacaat atcggccagg tcttccacgc gctgcaccag
     1741 gaaattatgc ttggtgatcg gctgcgtcag ccccagcacg tcggtttctt gaaaggcgtc
     1801 gcgcccgata aactgccgcg ccacctggcc ggtgatggcg acgatgggcg tcgagtccat
     1861 atacgctgtg gccagtcccg tcacgagatt ggtcgctccg gggccgctgg tcgccacgca
     1921 gacgcccacc ttgcccgtcg cgcgcgcgta gccgtcggcg gcgtgcgcgg cggcctgttc
     1981 gtgccgcgtc aatacgtgat gcagggttgg atacgctgtc agcgagtcat agaacggcat
     2041 gattgccccg ccgggatagc cgtacagcag ttcaaccccc tcgcgtatca gcgcctcaca
     2101 cataatctgg ctccctgcca gttcgcgcgc gtcgctttct tgctgcggtc gttcgacgtt
     2161 gtgctgcggc gacattgttg tcccgctctc ctggcgggtg atggttgcag cgacttgcat
     2221 gtttgctcct tctgatgttc cgtaccgatg atagttgatg ataccgaaaa acaataaaaa
     2281 aacgagaacc gtccgcttcg ggaggtcctc gttaggatac ctgctctgga ttggttgcga
     2341 cgttgcttcc gttaatctcc ctgtaatgcg ctcgcatcta gctctgtagt aagccctacc
     2401 agactcacaa gtccggtaag gccgctaagg gcgaggaggg ctacaaggag taggccgcga
     2461 agaataaaaa caaccggcgc aaacaaagaa accagcgcta atacgctggt ttcaatcatg
     2521 ctgggggttg cctgagtagc catgccagtc atacccgcat ggctcatggg agcctgctgc
     2581 tggcaagagg gcatacgttc cccatccatt cgatcggcag ccatcgctga tcgattgatt
     2641 gtggtccacg ttgccaacat ataactactc ctcaaataga atgctcaagg ccaaatttgg
     2701 cacaaaaaaa tacgtcacag atgatactcc ggcatcatcg ccgaagctgt ctgtacgcac
     2761 ttcatcgctt aaattgaaga tacactaagg agcaggggtt tgtcaacccc tgttccacca
     2821 acaattgcct gcaattgagc ctcttgcgcg aaaagagggt gttatttcgg caattgacgg
     2881 ctttcgataa tccgatcaat ggtcgattgc ctgacacggt aaacctcgcg ttttcctcga
     2941 tgtggaagcg acactgcttc taacgccccg tccttaatcc agcgccgcac ggtcgtttga
     3001 tcgacgcgca ggcgcgtggc tacttccctc actgttaaca agatgtctga cctctccgcc
     3061 atcaggatag ttcctccgtc gtctctgttt caggtgccca gcgacagcca ttgggataaa
     3121 ggtaccccta ctcttgcgct gtaccggcaa ggcaggtaca ctaacaaacc ggtttgacta
     3181 caatttgagc acacacaaac agctccatcg aaacacatcg atagagcgtt tataactcat
     3241 aaataacttc tgagaaattg taagctctac ataaagataa aaacacggca tattataggc
     3301 aagtttgaat agaagaacgt tgagcgtgta gagatttagc atatatggag aagagccggt
     3361 gaagaaggcc gaagatgcga ttcccaatga attgttgagt tttgagcgcg aacaacgcct
     3421 ctggacgcag gaggaggtgg cagagcatat tggcgcacct gatgcgaata tggttggaag
     3481 gtgggagcgc ggcattacac gtcctacttc gtattatcgt cagcaattat taacgctttt
     3541 cggcaagagc acccgcgagc ttggtttcgt tcgtaaaggg gagatcccat tctggcacgt
     3601 tccctatcgg caaaatgcgt atttcacggg gcgagacgaa ctccttcgac aaatacacac
     3661 tcacttcaaa caacagcagc gaaccaggcc gctgcccccg ctggcgctca gcggactggg
     3721 cggcgtcggc aagacccaga cagcgttgga gtatgcctat cgttaccgtc atgaatatca
     3781 tacggttgta tgggtccgcg ccgaatcttc cgaagcgctc atctcggact ttatcgcttt
     3841 cgcgggcctc ttgaatattc cagactgcca gcagcaagac catcaatcta ccatcaacgc
     3901 cgtaaaatac tggctttctg ccatgacgcg ctggctattt atctttgaca acgttgatga
     3961 tcttgccatg cttaacgatt ttctgccctc ggccccccgc ggtcatctcc tgctcacgac
     4021 gcgtatgcaa acgaccggaa cagccgcaga gaccctcgcc atagaatcga tgaatcctat
     4081 ggagggcgct tacttcctac tcaggcgttc gaaaattgtt ggctttaacg ccgctttcga
     4141 tgacatcggt aaagacgacc accacctggc tgtgagtatc tcggaaaagc tgggaggtct
     4201 tcctctggct cttgatcagg caggcgcgta catagaagaa acagcttgcg gacttgacgg
     4261 ctatctggag cgctatcagc agctgcgcga ggcatttctc caacgacggg gccacgccga
     4321 tcttcaccac cccgaacccg tcgccaccac ctggtcactt gcctttgaga aagcctctca
     4381 atcaaatatc gactcaattg cgctgctgcg cttcctggct ttcttctctc ccgatgcgat
     4441 ccctttgtag atcatcacca ccggatgcgc gctccttgaa ggatcgatcg cgacccttgc
     4501 cgcgcatcct ctcctcctcg atgaggcgat tcgagatcta cgccgctttt cgctcatcca
     4561 gcgtactgca accgccaata cccttttgct gcaccgcctc gtccaggtag tactccttga
     4621 cacgatgaca tctgaagagc agcgtcagtt ggcagaaatg cttgtgcgcg cgatcaatgc
     4681 tacgtttcca tcgggtgaat ttggaacgtg gcagcagtgc gagcgcctgc tctcccaggc
     4741 gcttagagga gtagaacttg ccaatcaatg gaacaccatc tcgccggaag cggcgcgcct
     4801 cacccaccac gtcgcgaatt acctgcgcaa tcgcgggcat ttcgccctcg ccgagcagct
     4861 ctaccagcag gcgctcgcgc agtatgaagc ggcgggtcaa tctgagctga gagaggtggc
     4921 ctcctgcctc tatcagatgg gagatctata tttaacgctc aaagattacg ataaaacgtt
     4981 tgctttcttt cagcgcgcag tggccattca ggaggaagcg ccgggcgaca tgcgtcccga
     5041 tctcgccaga acgcttgata ccattggaga agcgtactac tacctggagg actacgcacc
     5101 ggcagaagac tacttccggc gcgccctggt tcttcgccag gacatatttg gatcagatca
     5161 ccccgacacg gctgaaagtt taaataatat cggcatttta ctcctgaaat gcctgagaag
     5221 atacgacgag gccgagattg tctttcaaca ggtgctagcc attttgacga agcatcgcgg
     5281 acccgatcac aaggacgtgg gtatttgcat gctgaacctg gcaacaacct accgcgagca
     5341 aggaaagtac gaggtgggaa agcaatggta cctgcggacc atcgcgttat ggcggcgcat
     5401 cctgggcgag gaagacaccg atgttgcctt tgcgctcaga gcgctggcaa aactctacgc
     5461 cttagaggga tcagagcaag ccgaggcgac ttaccttcaa gcgctggcca tttatgaaaa
     5521 agcgcgtgga ccggagcatc gcgtgacgct agaggtgaag cgcgaactcg aagaactgcg
     5581 ccaggcgcca ttcagacacc tggcctcgtt caagggagca ggcgagtagc cagtttccaa
     5641 tccagcgcta caggctgctc attgcgatca ctttgctctt tgaggt
//
LOCUS       DASZAK010001299         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_68083, whole genome shotgun sequence.
ACCESSION   DASZAK010001299 DASZAK010000000
VERSION     DASZAK010001299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68083"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="VGT44_22815"
     CDS             <1..1303
                     /locus_tag="VGT44_22815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEV2583711.1"
                     /translation="SNWSGSVRGIPREVALPVSVDDLAQRIAGYARSGRHVRVVGSGH
                     SFTPLVRTDDVLISLDHMQGIETIDAEHGSVTILGGTKLKLLGEALLEKGLAQENLGD
                     INVQSIAGAVSTGTHGTGIRFGSLATQVQGFTLVTASGELLECSEEQNLDVFKAAQVS
                     LGALGVIARVKLRVVPAKVMHYQGLRKELDDCLAHLEQYKQENSHFEFFWFPYTKWVQ
                     AKFLNETSNPPDTNTFWSDFNKIVMENWVYGLLSRTVRRFPNLSQRACHISASSIANI
                     DETNYSHRLFSTPRSVRFQEMEYNIPAEQTVAVIHEIQECIAKRRFQVHFPLECRFVH
                     SDDMWLSPAYQRESAYLAVHMYQGMPYRDYFYHIEEIFKRYQGRPHWGKIHTRTAADL
                     AELYPHWHDFLRVRASLDPQGMFLNDYLRALFDVDGPVDAN"
     gene            complement(1401..1703)
                     /locus_tag="VGT44_22820"
     CDS             complement(1401..1703)
                     /locus_tag="VGT44_22820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583712.1"
                     /translation="MGTILSLLAVLGVATAVISIGLRISLYFYRQDALGMQPFVAQST
                     QATSTQQAILERSELLYRDEAISGNSRLAFIFLAFVAIVILLAILLIAGMASGFVP"
     gene            complement(1887..>2395)
                     /locus_tag="VGT44_22825"
     CDS             complement(1887..>2395)
                     /locus_tag="VGT44_22825"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV2583713.1"
                     /translation="PGMRKMTNLIQTDTSINPGNSGGPLVDSLGRVVGITTAMMPMAQ
                     GLGFSIPLDTVKSAIAHINQRRTSRPAVAGVSMGVGGMRVRLDDALLKGLNLAQQFGM
                     EVLEIRQGSPAERAELKRLDIIISADSDTVTQPGDLQRIVRRHRPGEKVPVSFLRGGK
                     LRKVTVIL"
BASE COUNT          534 a          632 c          681 g          548 t
ORIGIN      
        1 gagcaattgg tcgggttctg tgcggggaat accacgtgaa gttgcgttgc cggtcagcgt
       61 ggatgatctg gcgcagcgca tcgcgggtta cgcgcgcagc ggtcgtcatg tgcgggtagt
      121 cggctcaggc cattccttta ctccgcttgt gcgaacagat gatgtgctga tctcgctgga
      181 tcacatgcag ggtatagaga ctatcgatgc tgagcatgga tctgtgacga tactgggtgg
      241 cacaaaactg aaactgttgg gtgaggcatt gctggagaaa gggctggcac aggagaatct
      301 cggcgatatt aacgtacaga gcatcgcggg cgcggtcagc acaggtacac atggcaccgg
      361 catccgtttc ggttcgcttg ccacgcaggt gcagggtttc accttagtca cggcgtcggg
      421 cgagttgctg gaatgctcgg aggagcagaa tcttgatgtg ttcaaggcgg cgcaggtttc
      481 gctgggcgcg cttggcgtga tcgccagggt gaaattgcgc gtcgtgcctg ccaaagtcat
      541 gcactaccag ggattgcgca aagaactgga cgattgcctg gcccacctgg aacaatacaa
      601 acaggagaat agccacttcg agtttttctg gttcccctat acaaaatggg tgcaggccaa
      661 gtttctcaat gagaccagca atccacccga cacaaatacc ttctggagtg acttcaataa
      721 aatcgtgatg gagaactggg tctatggcct gctatcgagg acggtgcggc gcttcccaaa
      781 tctgagccag cgcgcctgtc atatctcggc cagctccatc gccaatatag acgaaaccaa
      841 ctatagtcac cgtctctttt caacgccgcg ctcggtgcgc ttccaggaaa tggaatacaa
      901 tatccccgcc gagcagaccg tcgcggtgat ccacgagatt caggagtgca tcgctaagcg
      961 ccggttccag gtacattttc ccctggaatg tcgtttcgtg catagcgatg atatgtggct
     1021 cagcccggcc taccagcgcg aatcggccta tctcgcggtg catatgtacc agggtatgcc
     1081 ctaccgagac tatttttacc acattgaaga aatcttcaaa cgctaccagg gccgtccgca
     1141 ctgggggaag atccataccc gcaccgccgc cgaccttgca gaactctatc cgcattggca
     1201 cgatttcctc cgcgtccgcg ccagcctcga tccacaaggg atgtttttga acgactacct
     1261 gcgcgcactc tttgacgtgg atgggccagt tgatgccaat taagaatgga gtgtcaccca
     1321 cgagaggtga cactccattt aataggccaa ttgcggattg acggatatcc tacgatggat
     1381 tagagacgta cattgatacg ctacggaacg aagccacttg ccatgcctgc gatgagcaga
     1441 attgccaaca ggatgacgat ggcgacgaag gccaggaaaa tgaaggcgag acgcgaattc
     1501 ccgctgatgg cttcatctct atacaatagt tctgaacgct ctaagatggc ctgctgagtt
     1561 gaagttgctt gcgtggattg agcaacgaac ggctgcattc caagcgcgtc ctgtctataa
     1621 aagtacaggc tgatgcgtaa tccgatggag atcacagctg ttgccacacc cagtactgca
     1681 agcaagctga ggatcgtacc cattgtcagt ttcccttccg gccttcttct ggcctcgcgt
     1741 ttagtgaacc agttcactaa cgttttgtga ctttgttcac tatgatagac gcgcgtttca
     1801 gggaaatctc aaaattttga gcggcaaatc gggcatgaga agctgtgtca cagatttgtc
     1861 agacgcatgc agttgatcgc tcggactcag agaataaccg tcactttacg tagcttgcct
     1921 cctcgcaaaa agctgactgg tactttttcg cctggacgat ggcggcgcac gatgcgctgt
     1981 agatcgcccg gttgcgtcac cgtgtcgctg tccgccgaga tgatgatatc caggcgtttg
     2041 agttcggcgc gctctgccgg gctgccctgg cgaatttcga ggacctccat cccgaattgc
     2101 tgggcgaggt tgagtccctt cagcaatgca tcgtccagtc gcacacgcat tccgccaaca
     2161 cccatcgaaa cacctgcgac tgctggcctg gatgtgcggc gctggttgat atgcgcgatg
     2221 gcggatttga cagtatcaag cgggatggag aagccaagtc cttgcgccat tggcatcatg
     2281 gcggtggtta tacccactac acgacccagg ctatcgacga gtgggccgcc ggaattgcca
     2341 gggttgatgg aggtgtcggt ctggatcagg ttggtcatct tgcgcattcc gggcg
//
LOCUS       DASZAK010001300         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_105536, whole genome shotgun sequence.
ACCESSION   DASZAK010001300 DASZAK010000000
VERSION     DASZAK010001300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105536"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..1231
                     /locus_tag="VGT44_22830"
     CDS             188..1231
                     /locus_tag="VGT44_22830"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2583714.1"
                     /translation="MTRAYANRIGLMADAPNFPAAWDEVVEKVKIADELGYDSIWLGE
                     SWGYELFTSMADLVRATKHIKIGAGIANIYSRTPALIASTVATLDERSGGRIVLGLGP
                     SGANVIEHWHGVPFQKPVQRTREYVEIIRMILRGEKLVYHGEFFNLERGFTLRFKPVR
                     KDIPIYIAAMGPKNVLQAGEISDGVLPVYWPANKWGDLRQQLDEGAKLAGRQPHSAAI
                     APYLTSFILDDNTSEEDRQSARFKAAMPLAYYIGRMGDYYAQMLARNGYGQEVEAVRD
                     AWKKGLQSAVEAVSPALLDITSLVGTPQEIVAKLDTWVAAGVDEPVLSMPPGTPDEVG
                     PQLQRLMQALTHS"
     gene            complement(1218..>2217)
                     /locus_tag="VGT44_22835"
     CDS             complement(1218..>2217)
                     /locus_tag="VGT44_22835"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEV2583715.1"
                     /translation="ALSWQPVAGWFPLQVLLFFLVVMLVYEAINAPISYYSGYVLPRR
                     YGLSVMTIAGWLRDVFIGLALELVLGIVLLEIIYFLLAAQPLTWWLWVALALLFFSVV
                     MANLAPVLLFPLFYKFHPLPEGDLSRRLLALAERAHTHVRGVYTFDMSRKTTAANAAL
                     MGLGNTRRIVLGDTLLKEFTNEEIEVVLAHELGHHVHNDIWKSLVMQSLLTLGGLYLI
                     NEIFHVAVDRLHLYQGLSDVATLPLLLALTTLFGLIVMPLNNAYSRLVEYQADEYALQ
                     STRMIEPFKGAMSRLANQNLAEAEPSPIVEFLFYGHPSIVKRLRHADAFQKAPTSG"
BASE COUNT          508 a          708 c          616 g          385 t
ORIGIN      
        1 gcttgcgtcg cccgcgtacc aggtggagcg aatgcatcgt gaaaccaaca ggctacgcgg
       61 gcgacgcaag cgtccccacc ctacaccacg tccacccccg cccctacggg caattcgtct
      121 cctaactttg tgatatactt gtggcacaaa acaggctcta cattcagcag gaaggaccag
      181 gcatctcatg acgagagcat acgcgaaccg catcgggctg atggccgacg cccccaattt
      241 tcccgccgcg tgggacgaag tggtcgagaa ggtcaagatc gccgacgaac tgggatatga
      301 ctcgatctgg ctcggcgaat cgtggggcta cgaactcttt acctcgatgg ccgatcttgt
      361 ccgcgccaca aaacatatca aaatcggcgc gggtatcgcc aatatttatt cacgcacacc
      421 cgccctgatc gccagcacgg tcgccacgct ggacgagcgt tccggcgggc gcatcgtcct
      481 cggcctgggg ccatcaggcg cgaacgtcat cgaacactgg cacggcgttc ccttccagaa
      541 gcctgtgcag cgcacgcgcg aatacgtcga gatcatccgt atgattttgc gcggcgaaaa
      601 gctggtctat cacggcgaat tcttcaacct cgaacgcggc tttaccctgc ggttcaagcc
      661 ggtgcgcaaa gatatcccca tttatattgc cgcgatggga ccaaagaacg tcttacaggc
      721 gggcgaaatc tccgatggcg tactgcccgt ctactggcca gccaacaaat ggggcgacct
      781 gcgccagcag cttgacgagg gcgcaaagct ggccggtcgc cagccccata gcgccgccat
      841 cgccccctac ctgacctcct tcattctgga tgacaacacc agcgaggaag accgccaatc
      901 ggctcgtttc aaggccgcca tgccgctcgc ctactacatc ggcagaatgg gtgactacta
      961 cgcccagatg ctcgcccgca atggctacgg ccaggaagtc gaagccgtgc gggacgcctg
     1021 gaaaaagggc ttgcagagcg ccgtcgaagc cgtctcgccc gcactgctcg acatcacctc
     1081 gctggtcggc accccacagg agatcgtcgc caaacttgac acgtgggtcg cggcaggcgt
     1141 cgatgagccg gtcctcagta tgccccccgg cacgcccgat gaggtaggac cacagttgca
     1201 gcgcttgatg caggcgctca cccactcgta ggagcctttt gaaacgcatc tgcgtgccgc
     1261 aaacgtttga caatagaagg atggccgtaa aaaaggaact ccacgatagg cgaaggctcg
     1321 gcttcggcca ggttctggtt tgccaggcgt gacatcgcgc ccttgaacgg ctcgatcatg
     1381 cgcgtcgatt gcagcgcgta ctcgtccgcc tggtattcga ccaggcgact gtaggcattg
     1441 ttgagcggca tgacgatcag cccgaagagc gtggtcagcg ccagcagtaa gggcagcgta
     1501 gctacatcgg acaatccctg gtaaagatgt agcctatcga ccgcaacatg gaagatctcg
     1561 ttgatgagat agaggccgcc tagcgtcagc agcgattgca tcacaagcga tttccagatg
     1621 tcgttatgca catgatgccc caactcgtgc gccagcacca cctcgatctc ctcgttggtg
     1681 aattctttca acagcgtgtc gcccagcacg atgcggcgcg tattgcccag gcccatcagc
     1741 gcggcattgg ctgccgtcgt cttgcggctc atatcgaacg tgtagacgcc gcgaacatgg
     1801 gtatgcgcgc gctccgccag cgccagcagc cgtcgcgata agtcgccctc cggcagtgga
     1861 tggaatttat agaacagagg aaagagcagt acaggcgcga ggttcgccat aacgaccgag
     1921 aagaagagca gcgccagtgc gacccacagc caccacgtca acggctgcgc cgccagcaag
     1981 aaataaatga tctccagcag tacaataccc agcacgagct ccaacgccag cccgataaag
     2041 acatcccgca gccagcctgc gatggtcatc accgataggc cgtagcgacg cggcaacacg
     2101 tatccgctat agtacgagat cggcgcgttg atcgcctcgt acaccagcat caccaccaga
     2161 aagaacaaca gcacctgcaa cggaaaccac cctgccaccg gttgccagct caaagcg
//
LOCUS       DASZAK010001301         1972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_316775, whole genome shotgun sequence.
ACCESSION   DASZAK010001301 DASZAK010000000
VERSION     DASZAK010001301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_316775"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VGT44_22840"
     CDS             <1..350
                     /locus_tag="VGT44_22840"
                     /inference="COORDINATES: protein motif:HMM:NF025681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583716.1"
                     /translation="QKHSPSWQAAYAKALAPFGLRGILSGDARKQTAWRVVDGAPVIQ
                     NLGLTSSVQMALDLIKRNTPARITRPHFVSLYMLAWNMTPSDVRTIAQSLDSRYEVVT
                     PGILLDMIARSSR"
     gene            347..1867
                     /gene="mftF"
                     /locus_tag="VGT44_22845"
     CDS             347..1867
                     /gene="mftF"
                     /locus_tag="VGT44_22845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03965.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HEV2583717.1"
                     /translation="MKTPSTHVPPPAGHYRLARGLRVRADHVICEYPLRVFRPGPATL
                     RLLEICAEARSPGELAAALGLPLKRVEQLCEQLRWRGLLEAGPPPPPQKWPGVSIVIP
                     TYNRAEQLERCLRALLQLEYPPEMLEIIVVDDCSTDQTAAIFTQLAPSFTSRGITLQF
                     LRHTLRQGAAQCRNNGAEAARFDLLAFLDSDCVVTHGWLTALVPAFADLALTAAGGQL
                     RAWERRSLSGRYEDRKSSLNMGAQPQRVTFDGPLTYLPTANLLVRREALRKIGGFALM
                     PFGEDVDVCRRLLLEGAHILYLPQSIVLHEYRTSLPAFLHTRVSYASSEAVLQRLHPA
                     TRRVLLLPPEQATFAGMAIGGLWGLLRMEMKRRGRAGVLALLTALLITLIGAGKRLRG
                     ARRLGIGLHPLSVLRATLRGNLAYTYHLCRHITRYYTLPLLAVGIIVPPLLLLLLILC
                     GIVIGVDYTRLRPNMRLGQFAICSLLDDCAYEVGVALGCVRQRTWMPLIPIVRKKL"
BASE COUNT          347 a          678 c          562 g          385 t
ORIGIN      
        1 tgcaaaagca ctccccatcc tggcaggcgg catatgcgaa ggcgctcgca ccgttcgggt
       61 tgcgcggcat cctgagcggc gatgcgcgca aacaaacggc atggcgtgtt gtcgatggcg
      121 cgccggtcat ccagaacctc ggcctgacta gcagcgtcca gatggcgctt gacctcatca
      181 agcgcaatac cccggcgcgc atcacgcgac cgcatttcgt gagcctctac atgctcgcct
      241 ggaacatgac gccatcggac gtgagaacca tcgcgcaatc gcttgacagc cgctacgaag
      301 tcgtgacacc gggtatactg ctggacatga tcgcgagatc atcgcgatga agaccccatc
      361 aacgcatgtt ccgccgccag caggacatta ccgccttgcc aggggcttgc gtgtgcgcgc
      421 cgatcatgtt atctgcgaat acccgctgcg cgtgtttcgc cctggcccgg caacgctgcg
      481 cctgctggaa atctgcgctg aagctcgctc gcccggcgaa ctggctgcgg cgctgggcct
      541 gccactcaag cgcgtcgagc aattatgcga acaactgcgc tggagaggac tgctggaagc
      601 tggcccgcca ccaccgccgc agaaatggcc gggcgtatcg atcgtcatcc ctacctataa
      661 ccgcgcagag cagctcgaac gctgcctgcg cgcgcttctc cagcttgaat acccaccaga
      721 aatgctggag atcatcgtcg tcgatgattg ctccaccgac cagaccgcag caatcttcac
      781 gcagctcgcg ccatctttta cctcgcgggg tatcactctg cagttcttac gccataccct
      841 gcggcaaggc gcagcgcagt gccgcaacaa tggcgcggaa gcggcgcgct tcgatctgct
      901 ggcctttctc gacagcgatt gcgtggtcac acacggctgg ctcaccgcac tcgttcccgc
      961 cttcgccgac ctcgccttga ccgctgctgg cggccaattg cgcgcctggg aacggcgtag
     1021 tctatcagga cgctacgaag acaggaaatc ttcgctgaac atgggcgcac agccgcagcg
     1081 cgtcaccttc gatggcccgc tgacctacct gccgactgcg aacctgctgg tacgccgcga
     1141 ggcgctgcgc aagattggcg gttttgccct gatgcccttc ggcgaggatg tagacgtttg
     1201 ccgccgcctg ctactggagg gtgcgcacat cctctatctc ccccagagca ttgtcctgca
     1261 cgaatatcgc acctcgctgc ccgccttcct ccacacgcgc gtctcctatg cctcctcgga
     1321 ggccgtcctg cagcggctgc atcccgccac gcggcgcgtc ctgctccttc cgccggaaca
     1381 agccacgttc gctggtatgg cgattggcgg attgtgggga ttgctgcgga tggagatgaa
     1441 acgacggggc cgcgccggcg tcctcgctct ccttaccgct ctcctcatca ccctgattgg
     1501 cgcgggcaaa cggctacgcg gtgctcgtcg cctgggcatc gggctacatc cgctgagtgt
     1561 actgcgcgcc acgttgcgtg gcaacctggc ctacacctat catctttgcc gacacatcac
     1621 gcgctactat accctgcccc tgctcgccgt gggaattatc gtaccgcccc tgctgttact
     1681 gctgctcata ttgtgcggca tcgtgattgg tgtggattac acccggctgc gtcccaacat
     1741 gcgcctgggc cagttcgcga tctgctcgct gctcgacgat tgcgcctacg aagtcggcgt
     1801 cgcgctgggc tgcgtcaggc aaaggacgtg gatgcctctg ataccgattg tcaggaaaaa
     1861 gctgtaatcc cggcgggtaa atatatttcc gtaggtgcca gttcaccgcg cacaccgccg
     1921 tgccagcccc agggcgaccg caagggtctc caccccactc ccgaccgctc cc
//
LOCUS       DASZAK010001302         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_175448, whole genome shotgun sequence.
ACCESSION   DASZAK010001302 DASZAK010000000
VERSION     DASZAK010001302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175448"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VGT44_22850"
     CDS             <1..198
                     /locus_tag="VGT44_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583718.1"
                     /translation="LSATMMIYGNPSGPINRRCARETGTYGDAVILTLGVRHRIHMKF
                     VGLLATPSIMHEILLAGNMLF"
     gene            270..2033
                     /locus_tag="VGT44_22855"
     CDS             270..2033
                     /locus_tag="VGT44_22855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEV2583719.1"
                     /translation="MSDPSVIHNAPAGQQNLPDVGRPWTRHYARGVPAVIDIPDHPLT
                     WLLDSTVSTYPSHTAFIYYGTKLSYAQFSSLANRFAMALQRLGIEPGDRVAIALPNMP
                     QYPIAYYGALRAGAVVVPTNPLYTEREMQHQLADSGARAVVMLDMFYPVVRAVREKTA
                     LEHFILTSPADYLPPLLHRLYPLSQRNAKHPEPPLTEKELREDKSLHIMQNLLEAHTK
                     GGIEVYNLPVAARGDDLAVLQYTGGTTGLSKGAMLTHRNLLANTLQTRMWVVDARDAQ
                     EISLCVAPFFHSYGMTACMNLSIAAAATMILLPQFKVKEVVTAIRRYHPTQLPGIPTM
                     YLSIMKEMGSHSQELRSIKYCISGASALPAKVRIDWERLTGGKLVEGYGLSEAAPVTH
                     CNPLNGDIREGSIGLPLPDIDSVILDEQTGKPLPVGEAGEIAVKGPNIMRGYWNQPGE
                     TADIFVHGWMRTGDIGKMDEDGYFYVVDRAKDMIKASGFNIFPREVEEVLFHHPAVQE
                     AAVVGVPHEYRIETVAAVIVLKPGYEPSDATRQDILAFCKKELTPYKVPKIVEFRESL
                     PKSLIGKVLKRELRETIKKPS"
BASE COUNT          486 a          669 c          634 g          446 t
ORIGIN      
        1 ttatcggcca cgatgatgat atacggcaat ccatccgggc cgataaatcg gcggtgtgcg
       61 cgggaaaccg gcacctacgg agatgcggtc attttaactt tgggcgttcg tcatcgcatc
      121 catatgaaat ttgtcggatt gttagctacc ccttctatta tgcacgaaat tttgttagct
      181 ggcaacatgt tattctgaat gcagccgcgc tatactggta ctatgtaatc ctcgtcggtt
      241 gtcgcggctc agagaggagc ttgcaaggaa tgtctgaccc atcggtaatc cacaatgcgc
      301 ccgccggaca gcagaatcta ccggatgttg ggcgtccctg gacgcgccat tacgcgcggg
      361 gcgttccggc tgtcatcgat atccccgatc atcccctgac ctggctgctc gactcgaccg
      421 ttagcacgta cccttcgcat accgctttca tctactacgg cacgaagttg agctacgcgc
      481 agttttcgtc gctggcgaat cgcttcgcga tggcgctgca gcggctgggc atcgagccgg
      541 gcgaccgcgt ggccatcgcg ctgcccaaca tgccacaata cccgatcgcc tattatggcg
      601 cgctgcgagc cggggcagta gtcgtcccca ccaatccgct ctataccgaa cgcgagatgc
      661 agcaccagct cgccgactca ggcgcgcgcg cggtcgtgat gctcgatatg ttctaccccg
      721 ttgtgcgcgc cgttcgcgag aaaaccgccc tggaacactt catcctcact agtcccgccg
      781 attacctgcc gccactcttg cacaggctct atccactcag ccagcgcaat gctaagcatc
      841 ccgaaccgcc tctgaccgag aaagaattgc gcgaggacaa gtcgctgcac atcatgcaaa
      901 atctgctcga ggcgcatacg aagggcggca tcgaggtcta caacctgccg gttgcggcgc
      961 gcggggatga cctggcggtg ctgcaataca caggcggcac gacgggtctg tcgaagggcg
     1021 cgatgttgac gcaccgcaac ctgctggcga acacgctgca aacccgcatg tgggttgtgg
     1081 atgcgcgcga tgcccaggag atcagcctgt gcgtcgctcc gttcttccac tcttatggta
     1141 tgacggcctg catgaatcta tccatcgcgg ctgctgccac gatgatactg cttccgcagt
     1201 tcaaggtcaa agaagtggtg actgccatcc ggcgctacca tccgacgcag ttgccgggca
     1261 ttccaaccat gtacctctcg atcatgaaag agatgggcag ccatagccag gaattgcgct
     1321 cgatcaagta ttgcatcagc ggcgcgtcgg cgctgcccgc gaaggtgcgc atcgattggg
     1381 agcgactgac cggcggcaag ctggtcgagg gctacggcct gagcgaggca gcgcccgtca
     1441 cgcactgcaa cccgctcaac ggcgatattc gcgagggcag catcggcctg cccctgcctg
     1501 acatcgatag cgttatcctg gacgagcaga ccggcaaacc tttgcccgtt ggcgaggccg
     1561 gagagatagc ggtcaagggt cccaacatca tgcggggcta ctggaaccag ccgggcgaga
     1621 cggcggacat cttcgtacat ggctggatgc gcaccggcga tatcggcaaa atggacgaag
     1681 acggctactt ctacgtggta gaccgcgcca aagacatgat taaggccagc ggcttcaaca
     1741 tcttcccgcg cgaagtcgaa gaagtgctgt tccaccaccc cgccgtgcag gaggccgccg
     1801 tcgtgggcgt accgcacgaa taccgcatcg agacggtcgc ggcggtgatc gttctcaagc
     1861 ccggctacga gccgagcgac gcgacgcgcc aggatatcct ggccttttgc aaaaaggaat
     1921 tgacccctta caaggttccc aaaattgtag aatttcgtga aagtttaccc aaatccctca
     1981 tcggcaaagt cctgaaacgc gaattgaggg agaccatcaa aaaaccttcg taggtgccga
     2041 tttatcgcgc acaccgccga tttatcggcc tggatagttg ccgttgatgc ccataggggc
     2101 cgataatgaa cattaacaaa ttaagttgga gataagccaa catagtggag cagatccgat
     2161 gagggctgat cgtactgatc gagccagtcc tggacgcgct cttgcaagtc cttccagcga
     2221 ctgctataac gatgc
//
LOCUS       DASZAK010001303         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_389611, whole genome shotgun sequence.
ACCESSION   DASZAK010001303 DASZAK010000000
VERSION     DASZAK010001303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389611"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            214..1239
                     /gene="pdhA"
                     /locus_tag="VGT44_22860"
     CDS             214..1239
                     /gene="pdhA"
                     /locus_tag="VGT44_22860"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEV2583720.1"
                     /translation="MAISNATLAGLTRTQLLEMHYRMALLRRFEEKSAEEYTRGKIGG
                     FMHLYIGQEAIGVGVMASARQQDKVLASYREHGHAIAKGMAPGAIMAELFGKSDGCSK
                     GKGGSMHMWSNDYGMFGGNGIVAAQLPIAAGVAFAEKYQGTDNIVVCFFGDGAVDEGA
                     FHEALNLASVWKLPVLYICENNQYSMGMAVEKAWAVDSLLPRAAAYNMPFEQVDGMDV
                     VTVYETAKRAIEYTRSGKGPYLLEIKTYRFRGHSMTDPAYYRTREEEQQWRNTRDPIG
                     IFEKKLLELGVATQADFDDNDARATQDAEDAADFAENSPFPTPGELYSDVMVDDSTAL
                     TYRHDRS"
     gene            1262..2263
                     /locus_tag="VGT44_22865"
     CDS             1262..2263
                     /locus_tag="VGT44_22865"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV2583721.1"
                     /translation="MRTITYRDALREAIREEMLRDERVFLMGEDIAGYGGTYAVSKGL
                     FEEFGEKRVRDTPLAEEVITGAAIGAAVNGLRPILEIMTINFSLLATDMIINNAAKFR
                     YMFGGQPSVPLVIRMPGGGGAQRAAQHSQMLETFYAHIPGLKVVMPATPYDAKGMLKA
                     AVRDPDPVIFIEHELLYNVKGEVPDEDEEYIVPLDRGEVKREGRHVTIVSFSRVFHLA
                     MQAAEDLAREGIEAEVIDLRSIRPVDIDLLVDSVKKTNRVVIAEEGWKYYGTGQGLAA
                     LIYEFAFDYLDAPIQHISGADVPMPYSKPLERAALPNKGDIINAVKAIVPAAIAGKS"
BASE COUNT          533 a          635 c          676 g          434 t
ORIGIN      
        1 agggcgtccc caagaggacg ccctttgacc acacctggct ttctccccgt tataggtttc
       61 catatacctc tcaccctata catcccaccc ggcatggggt aaaatgtatt ccagaacaag
      121 cacactgacg tgacctcatg gtcctggcgg agacaacgta caacgttgtg tacagataga
      181 acaaacactg aaggaagtac agggagaggg cttatggcga taagtaatgc aacgctcgcg
      241 gggcttaccc ggacgcagct gctggaaatg cactaccgca tggcgctgct gcgtcgattt
      301 gaggagaaaa gcgcggagga atacacgcgc ggcaaaatag gtggattcat gcatctctac
      361 atcggccagg aggccatcgg tgtaggagtg atggcctcag cgcgccagca agataaagtg
      421 ctggccagtt atcgcgaaca tggtcacgcc attgcgaagg gtatggcgcc tggcgcgatc
      481 atggcggagt tatttgggaa aagcgatggg tgcagcaagg gcaagggcgg ctcgatgcat
      541 atgtggagca acgattacgg catgttcggc ggcaatggca tcgtcgcggc gcagctgccc
      601 atcgcggcag gcgtggcctt cgccgaaaag taccaaggga cagacaacat tgtcgtttgc
      661 ttctttggcg atggcgcggt agacgagggt gcgttccatg aagcgctgaa tctggcttcg
      721 gtctggaagc tgccggtgct gtatatttgc gaaaataacc agtattcgat gggcatggcc
      781 gttgaaaaag cctgggccgt cgattcactc ctgccgcgtg cggcagccta caacatgcct
      841 ttcgagcaag tcgatggcat ggatgtcgtc accgtctacg aaacggccaa acgcgccatc
      901 gagtatacac gcagtggcaa gggtccctat ctgcttgaaa tcaagaccta ccggttccgc
      961 ggccactcca tgaccgaccc ggcctattac cgcacgcgcg aagaagagca gcagtggcgc
     1021 aacacacgcg atccaatcgg catcttcgag aaaaaactgc tggaactcgg cgtcgcaaca
     1081 caggccgatt ttgatgacaa cgatgccaga gctacccagg acgccgaaga cgcggcggat
     1141 ttcgccgaga acagcccctt tccaacaccc ggagagctgt atagcgatgt gatggtggat
     1201 gattcaaccg ccctgactta ccggcacgat cggtcataac tcacaacgta taaggagcag
     1261 tatgcgtacc ataacctacc gcgatgcgtt gcgcgaagct atccgcgaag agatgctgcg
     1321 cgacgaacgt gtttttctga tgggcgagga catcgccggg tatggcggca cctacgccgt
     1381 cagcaaaggt ttatttgagg agttcggcga gaagcgtgtg cgcgataccc cgctggccga
     1441 agaagtcatc accggagcgg ccatcggcgc ggccgtcaat gggctgcgcc caatcctgga
     1501 aatcatgacg atcaatttca gcctgctcgc caccgatatg attatcaata acgcggccaa
     1561 attccgctat atgtttggcg gtcagccatc ggttcccctg gtgatacgca tgcccggcgg
     1621 cggcggcgca cagcgcgcgg cgcagcactc gcagatgttg gagacgttct acgcgcatat
     1681 ccccggcttg aaagtcgtga tgccggccac gccctacgat gccaaaggca tgctcaaggc
     1741 cgccgtgcgc gatcccgatc cagtcatctt catcgaacac gagctgctct acaacgttaa
     1801 aggcgaagtg ccggacgaag acgaggagta tatcgttcct cttgatcgcg gcgaggtgaa
     1861 gcgcgagggc agacacgtca cgatcgtctc cttctcgcgt gtattccacc tggcaatgca
     1921 ggcagcagag gatctggcca gagaaggcat cgaagccgag gtgatcgacc tgcgtagcat
     1981 tcgtcccgtc gatatcgatc tgctggttga ctcggtgaag aagacgaacc gcgtcgtgat
     2041 cgccgaagag ggctggaaat attacggcac gggccagggg ctggctgctt tgatctacga
     2101 gttcgccttc gactacctgg acgcgcccat ccagcatatc agcggcgcgg atgtccccat
     2161 gccctactcg aagccattgg agcgagccgc actgcccaat aaaggcgata tcatcaacgc
     2221 ggtcaaggcg attgtgccag ccgccatcgc aggcaagtca taagactgta ggggcgag
//
LOCUS       DASZAK010001304         4046 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_209455, whole genome shotgun sequence.
ACCESSION   DASZAK010001304 DASZAK010000000
VERSION     DASZAK010001304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209455"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1848)
                     /locus_tag="VGT44_22870"
     CDS             complement(<1..1848)
                     /locus_tag="VGT44_22870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEV2583722.1"
                     /translation="MMMEVAQHTGQLNAAEVLAALLDELGAVTDYRTLRDTLPRRLAR
                     LLQCRCIIVYQRYGDTLQFVSGSFDDQPGWSSALLSVTHINPVSVHGDTLEARAWQQR
                     KAVVEPASLATRVATPLLYRMRGIGVLVAIRGEQPPTGTQSRHDPSAAIVHYPDYWTQ
                     NDAQIVEAITGVVAMLLENARMLERDRDRIQELSLLNSISRQFHASLHNRERVRNHII
                     QRTREISSADLCDCLLPGSGQEYSEWLSPALQELLLRRMAEQHDAPLIIERPGDAHTS
                     PLVEHLDPSIKTFFAIPLWSGRSSESVAGSPTTSRLLGMIAGGYRRPWKLRREEIILL
                     NVLANQASSVIENITLMAEVIEARNEARKLLRKVLEDQRQQELILESIPSGLLTIDLH
                     ERITTCNRAAAAMLDCHAFEAIGQPAKKLLALRTFQQVIQSELPQSETLTISPAQGQE
                     RIVQVTLLPFRSDQGELMGALATLTDMTTTHRLEEEKRRLDRLALLGEMAANVAHEVR
                     NPLASIKTSLQMLLDDLDHQPPLPSNNATDLDDEETDGMSDTVKVALKEVERLHAIVH
                     DLLQFSRPSQLHRVRCSLAEISDRLLHMLEKQAMEAGIFIHRLYRDSPPI"
     gene            complement(1961..3247)
                     /gene="codA"
                     /locus_tag="VGT44_22875"
     CDS             complement(1961..3247)
                     /gene="codA"
                     /locus_tag="VGT44_22875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine deaminase"
                     /protein_id="HEV2583723.1"
                     /translation="MQYDLIIRHAQLHRRTGLVDIAIQDGRFARIALDIPATALHEID
                     TAGRLVSPPFIDAHVHLDAVLTVGQPRYNTTGTLLEGIQIWSERKPSLTREDVKRRVL
                     EEIRWEVAQGTLHIRSHVDVCDPELTALKALLEVREEVRDICDVQLVAFPQDGILSFP
                     NGPELMRRAMELGCDLVGGIPHYEWTRDMGVEDVHVAFALAKEFNRDIDCHIDETDDP
                     HSRFTEVLAADTIQQSWQGRVTASHCTAMHSYDNAYAFKLLRLLARAQVNVVANPFDN
                     VVLQGRFDTYPKRRGIARVKELLASGVNVALGHDSIMDPWFPLGRGDMLAAAQLALFL
                     CHMSGYEEINDVLDLITTNAAKALRITDRYGIEEGKPADFLVLDAPSAFEALRLVPAR
                     LRVFKQGREVANTTPARSVLMRQGEVEGTPVTYCVG"
     gene            complement(3381..>4046)
                     /locus_tag="VGT44_22880"
     CDS             complement(3381..>4046)
                     /locus_tag="VGT44_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583724.1"
                     /translation="LGMAFTTGIANVAAWSAGFGKSGDVGGLLSAVVSPLGGFGKFLL
                     LLLALSIVANNIPNDYSLGLSVQVFGKAFQQVKRYVWTLIGAVIYIVIAVIGSANFTA
                     TLSSFLLDVAYWLGAYAIILVLEHFVFRRGQYNVDDWNTASKLPLGWAAIVALAAGLV
                     GALLGADQLNFQGFITRHINQPLGIDLGFELSVLLAGVVYLILRTIEIRTTPRGEAAA
                     GGK"
BASE COUNT          860 a         1102 c         1227 g          857 t
ORIGIN      
        1 gatgggaggc gagtcgcggt aaagacgatg tatgaagatg cccgcttcca ttgcttgctt
       61 ttcgagcata tgcagcaggc ggtcgctgat ctccgcgagc gagcatcgca cccggtggag
      121 ctggctcggt cgtgagaatt gcaggagatc atgcacaatg gcgtgcaggc gctctacttc
      181 tttcagggca accttgaccg tgtcactcat cccatcagtc tcttcatcgt caaggtctgt
      241 agcgttgttg gaagggagag gtggctgatg gtccaggtca tccagtagca tttgcaggga
      301 tgttttgatc gaagcgagcg gattgcgcac ttcatgggcg acgttggcgg ccatttcacc
      361 aagcaatgcc aggcggtcga ggcggcgttt ctcttcttcc agccggtgcg tcgtggtcat
      421 gtctgtcagg gtggccagcg cgcccatcag ctcaccctgg tcgctgcgga agggcagcag
      481 tgtcacctgc acgatgcgct cctgtccctg cgcaggcgaa atggtcagcg tttcactttg
      541 tggaagctct gattgaatga cctgctgaaa tgtgcgcagc gcaagcaact ttttcgccgg
      601 ttggccgatg gcttcaaagg catgacaatc cagcatggcg gcggcagcgc ggttgcaggt
      661 ggtgatgcgt tcatgaagat caattgtcag caggccgctg gggatgcttt ccaggataag
      721 ttcttgctgg cgctggtctt cgagtacctt gcgcagcaac ttgcgcgcct cgttgcgcgc
      781 ctcgattacc tccgccatca aagtgatgtt ttcaatgacc gaacttgcct gatttgccag
      841 cacgttgagc agaataatct cttcgcggcg taacttccag gggcggcgat agccaccggc
      901 aatcatacct agcaggcgag aagttgttgg ggagccggcc acgctctccg aactacggcc
      961 actccataat ggaatggcaa aaaaggtttt gatggatgga tcgaggtgtt ccaccaacgg
     1021 gctggtgtga gcgtcgccag gtcgctcgat gataaggggt gcgtcgtgtt gttcggccat
     1081 gcggcgcaac aggagttctt gcagcgctgg tgaaagccac tcgctgtatt cctgccctga
     1141 accgggaagc aggcaatcac agagatcggc tgagctaatc tccctggtac gctggatgat
     1201 atggttgcgc acacgctcgc gattgtggag cgaggcgtga aactggcgac tgatgctgtt
     1261 gagcagcgat aactcctgga tgcggtctcg gtctcgttcg agcatacgcg cattttcgag
     1321 cagcatggcc acgaccccgg tgatggcttc gacaatctga gcgtcgttct gcgtccagta
     1381 atcgggatag tggacgatgg cggctgacgg atcatggcgc gattgtgtcc cggtaggcgg
     1441 ttgttcgccg cgaatcgcta ccagtacgcc gatgccgcgc atacggtaga gcagcggtgt
     1501 ggcgacgcgg gtagcaagag atgctggttc aacgacggcc ttgcgctgtt gccatgctcg
     1561 cgcctcaagc gtgtcgccat gcacactgac cggattgatg tgcgtgacag acagcagcgc
     1621 gcttgaccag ccgggttggt catcgaacga gcctgagacg aattgcagtg tatcaccgta
     1681 gcgttggtag acgatgatac atcgacattg taatagtcgc gccaatcgtc gtggcagcgt
     1741 atcgcgaagc gtgcgatagt ctgtgacagc gccaagttca tcgaggagag ctgccaggac
     1801 ctcggctgcg ttcaattgcc cggtgtgctg cgccacttcc atcatcattg cttcccccca
     1861 cgcctattat gagtaaccct acgaactatc cagccccact atagcagaaa accggctgta
     1921 ttgcacgcgc aaacggcaaa aatgaaggaa ttgtcatatg tcagccgacg cagtaggtta
     1981 ccggtgttcc ttcgacttct ccctgtctca tcaagacgct tctcgcgggt gtcgtgttgg
     2041 ccacctcgcg cccttgcttg aagacgcgta gacgcgcggg aaccaggcgc agggcctcga
     2101 acgcggaggg tgcatccagc acgaggaaat ctgccggttt gccttcttcg atgccgtaac
     2161 gatccgtaat gcgcagcgcc ttcgccgcgt tggtcgtaat caaatccagc acatcgttga
     2221 tctcttcgta gccgctcatg tggcagagga acagggcaag ctgcgcggcg gcgagcatgt
     2281 cgccccggcc aagcgggaac caggggtcca taatcgagtc gtgtccgagc gcaacgttca
     2341 cgccactggc cagcagttcc ttgacacgcg caataccgcg ccgtttgggg taggtatcga
     2401 aacggccttg cagcacgacg ttgtcgaatg ggttggctac cacgttgacc tgggcgcgag
     2461 cgagcaggcg cagcagcttg aaggcgtagg cgttgtcgta ggaatgcata gcggtgcagt
     2521 ggctggccgt gacgcgccct tgccagctct gctggatcgt gtcggcagcc aagacctcgg
     2581 tgaagcgcga gtggggatcg tcggtttcat cgatatggca gtcaatgtca cggttgaact
     2641 cctttgccag cgcgaaggcg acatggacat cctcgacgcc catgtcgcgt gtccattcat
     2701 agtggggaat gccgccgacc aggtcgcagc ccaattccat tgctctgcgc atgagttcgg
     2761 ggccattggg gaaggaaagg atgccgtcct ggggaaaggc gaccagttgc acatcgcaga
     2821 tatcgcgcac ctcttcgcgc acttcgagca acgctttgag cgcggtcagt tcgggatcgc
     2881 acacatcgac gtgactgcgg atatgcagcg taccctgggc tacttcccag cgaatctctt
     2941 ccagcacccg ccgcttgaca tcctcgcgcg taagcgaagg cttgcgctcg ctccagatct
     3001 gtatgccttc gagcagcgtt ccggtcgtgt tgtagcgtgg ttgtccgacc gtcagcacag
     3061 catccaggtg aacatgcgca tcgatgaagg gcggagagac caggcggcct gccgtatcga
     3121 tttcgtgcag cgccgtagcg gggatatcca aggcgatacg cgcgaaacgc ccatcctgaa
     3181 tagcgatatc gacgagtccc gtgcgtcgat gcaactgcgc atggcggatg atgagatcgt
     3241 attgcataat attcttcctc ttgtaaagag tgctacctta tccagagact gatgatagat
     3301 agcgagaaat ggtgtgctga aaatacgagg caggcggtta ttacgaaccg cctgcccgga
     3361 acggaaacaa ggtcaatgtg ctactttccg ccagcggcgg cttctccacg tggagtggtc
     3421 ctgatttcga tggttcgcag gatgaggtag acgacaccgg ctaagagaac tgacagctcg
     3481 aagcctaagt caataccaag gggctggttg atgtgccggg taataaaacc ctggaagttg
     3541 agttgatcag cgcctagcaa agcgccgacc aggcccgcag cgagagctac aatcgcagcc
     3601 caacctaacg ggagcttgct ggcggtattc cagtcatcga cgttatactg gccacgcctg
     3661 aacacaaaat gttcgaggac gagaatgatg gcataagcgc caagccagta ggccacgtcg
     3721 agcaggaagc tcgacagtgt agcagtgaaa tttgctgagc cgatcacggc tatgacgatg
     3781 tagatcactg caccgatcag cgtccagaca tagcgtttga cctgctgaaa tgctttgcca
     3841 aacacctgca ccgagaggcc aaggctataa tcgttgggaa tgttgttggc aacaatgctg
     3901 agcgccaata gcaggaggag gaatttccca aagccaccga gcgggctaac gactgcagaa
     3961 agtagcccgc cgacatcgcc ggacttacca aagccagcgc tccaggctgc cacattagca
     4021 atgcccgtcg tgaatgccat tcccag
//
LOCUS       DASZAK010001305         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_105827, whole genome shotgun sequence.
ACCESSION   DASZAK010001305 DASZAK010000000
VERSION     DASZAK010001305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105827"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..479)
                     /locus_tag="VGT44_22885"
                     /pseudo
     CDS             complement(1..479)
                     /locus_tag="VGT44_22885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041836501.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            786..1664
                     /locus_tag="VGT44_22890"
     CDS             786..1664
                     /locus_tag="VGT44_22890"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2583725.1"
                     /translation="MSLEVRELGHITFYEAVPPNPANNTTYFLLHGIGTSLDFWVAVA
                     PLLGKENRTIAIDIPGFGRSSLPKDGPILEAAVRQISEFTRSLDVNNGILVAHSLGAF
                     VALRLAAVERSRFQRLILVDGTLGRALQLIKNPRSILCQPALGAIVSTQFLAGTLPLK
                     KNLTSIIGHSSLLRSLTLRPWVDEPGHLDPGIVTAALSNNGGLGSLRSLAAARQIEYE
                     DLLRGVTQPVDLVWGERDNLINASDIEYVASLVNVDRRMAIPRCGHWPMIEYPIVLAQ
                     FISAWNEDGKVEDRNN"
     gene            1892..>2960
                     /locus_tag="VGT44_22895"
     CDS             1892..>2960
                     /locus_tag="VGT44_22895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583726.1"
                     /translation="MVDQRLKVNDLDPFEFMLLYRGLKKMHTAWLNVIALVVVLMVFF
                     ATCTDGTVGAFRDFQFWADIQRVLGIATKPPSPPDFPFIRDVTSWFLLVIVISGALLL
                     HRQWQYASRCLSSLAKNGAIVPRTQPVSNVFSRVLRIDKIISGAPPEQTFNVLVDRVV
                     FCLSRRSAWLAASMIVTSLILTELLVLGQQHGLFQALAPHGLTPGERQVWLNQAYESW
                     WASRYHVFGYLIYQVLALFGIFVILNFDVVGVAIMYVIIAMHFLMEPRADWLNRDGRF
                     GWSPVARVYRIAIWANALLGAALTAVLLTLGIHNYNWVAVLVIVYMIFMPMFILTPWL
                     VFRHVEVAARTKRISEIEEVMT"
BASE COUNT          640 a          796 c          811 g          713 t
ORIGIN      
        1 ccactcggct ttcttgtcgt gcacagcgcc gggcagcgcc ccggacaccc acacgatgtc
       61 cccggccggg ctggcgatga cctgcaggtt catcccgtgc ttcttgtgct tgccggagca
      121 ggacggccgg tccgcggcga cccggccggc cgggatcagc gtcccgtcca ggaccacgta
      181 tgcccatccg gcctttaccg cgccgcggat cgcctggcgc agcttcggcg cccgggccgc
      241 cagcagcgcc acggtctcgt tcacataccg ccaggccgta gaggtgccga ccccgaaccc
      301 ggcggccagc tcatcgaacg tctcgccttt gcgcaggtaa acgagcacca gcagggcctg
      361 ctgcccggag ttcagcttct gccagcacga ccgatcgcaa cccggtgacg gcggatcatc
      421 ccggacacaa aggtgagggt ccgggacgac aacggcagcg cggaacggta gaaaagcatg
      481 gaagcccctg gtgagagtga agccaggttt tggtcgactg cctcacttac caggggttgg
      541 gtatcagtct cagcgtcgac acgccgcgca gtgaccatcc tgtgacacac gggctagatc
      601 aggccagatc agggatgaaa acgctcaatg aagatctgcg agccgaacgc aagatgaagg
      661 ggctcactgg atatgcgggc ttccgaacgg tcgggtgctc actacagcat tctgagctag
      721 ggcttggtat cattacgata cgatggctgc gcattccttg tccacgtccc atgcagaggt
      781 agccgatgtc acttgaagta agagaacttg gtcatatcac gttctacgag gcagtaccgc
      841 ccaatcctgc aaacaataca acctactttt tgcttcacgg cattggcaca tctctagact
      901 tctgggtagc ggtcgctccg ctattgggga aggaaaatcg aaccattgca atcgacattc
      961 ccggcttcgg aagaagttcg ttgcccaagg atggacccat acttgaagcc gctgtacggc
     1021 aaattagcga attcacacgc tcgctcgatg tcaataacgg tatattggta gcccattcac
     1081 ttggagcatt cgtcgcgcta cgtcttgctg ccgtagagag gagccggttc caacgtctga
     1141 tactagttga tggtacgctg ggccgggccc ttcagcttat caaaaacccg cggtcgatac
     1201 tttgtcagcc ggcacttggc gctattgtct caacgcagtt tttggctgga acccttccgc
     1261 tgaagaagaa ccttactagt ataataggtc attcttcatt gcttcgcagt ctaacgctaa
     1321 ggccgtgggt cgacgagcca ggccacctgg atccaggtat agttactgct gctctttcca
     1381 ataatggcgg ccttggctcc ttgcggagct tggctgcggc gcggcagata gagtatgaag
     1441 acctgttgcg cggagttact caacctgtag atttggtctg gggcgagcga gataacttga
     1501 taaatgccag cgacatagag tacgtagcga gcctagtaaa tgtcgatagg cgcatggcaa
     1561 ttccccgatg cggtcattgg ccaatgattg aatacccaat cgtgctagct caatttatta
     1621 gcgcctggaa tgaagatggc aaagtagagg atcgaaataa ctaaatgtga cgatagcagt
     1681 actctacgtg ttagcagggg agcgacttct ccggtcataa gacgcttcca aggcgaccgg
     1741 cggccgtgtc cggtttgttc taacacttct aagagattgc cggtttatct tgtctctctc
     1801 gcgtggccgc tgccgacacg tagacttcaa gttactaaag cttacgtcgg cgcctacgaa
     1861 ggcctaagct ttctgatgtc atggagcaca gatggtagac caaagactca aagtcaatga
     1921 tctcgatcca ttcgaattca tgctgcttta tcgcgggctc aagaagatgc atacggcctg
     1981 gctgaacgtt atcgctctgg tagttgtgct aatggtattt tttgccacgt gcactgatgg
     2041 taccgtggga gcgttccgcg acttccagtt ttgggctgat atccagcgag tgctgggcat
     2101 tgcaactaaa ccgccttcac cgccggattt cccgttcatc agagatgtga cgagctggtt
     2161 tttgctggtt atcgtaatat ctggcgcact cttgctacac cgccagtggc aatatgcatc
     2221 acggtgccta tcaagccttg caaagaacgg tgcaatagtc cccaggacgc aacccgtctc
     2281 gaacgttttc tcgcgcgtgc tcaggatcga taagataatc tccggcgctc ctccagagca
     2341 aactttcaat gtgcttgttg accgggtcgt tttctgcctt tcgcggcgca gcgcatggct
     2401 tgccgcctca atgattgtga catcgcttat cctgaccgaa ctcctcgtct tggggcaaca
     2461 gcacggcttg ttccaagctc ttgctcccca tggtttaact cccggtgagc ggcaagtatg
     2521 gctgaaccaa gcgtacgaaa gctggtgggc gagtaggtat cacgtctttg gctatctaat
     2581 atatcaggtg cttgctcttt tcggaatttt tgtaattctt aatttcgacg tggttggcgt
     2641 tgctattatg tacgtgatca tagcaatgca cttcctaatg gaaccgagag ctgattggct
     2701 caatcgtgac ggacggttcg gctggtcacc agtagcacgc gtatacagaa ttgctatttg
     2761 ggcgaacgcc ctcctgggtg cagcactaac ggcggttctc ctgacccttg gtattcataa
     2821 ttacaactgg gtcgcagtgc tagtgattgt ttatatgatt ttcatgccaa tgttcattct
     2881 aactccatgg cttgtatttc ggcatgtgga ggtggcggca cgtacgaagc gaatttccga
     2941 gattgaggag gtaatgacca
//
LOCUS       DASZAK010001306         5613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_565586, whole genome shotgun sequence.
ACCESSION   DASZAK010001306 DASZAK010000000
VERSION     DASZAK010001306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565586"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2181)
                     /locus_tag="VGT44_22900"
     CDS             complement(<1..2181)
                     /locus_tag="VGT44_22900"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583727.1"
                     /translation="MKTSMYFNYTTRSLMRGGQRTVLAIFCVAVGVMAIVALQLVGLM
                     ISGALTSNVRDANGGDIAVTSTTKPFTNDDLSYFSQLKSQGTITDYTAVINSTGSMGN
                     SASITDSFTIQAVDAATFPIVTPPTFVEPSNGSIASLLTGNGVIIDKTLADQFHKQVG
                     DTFDVHVGSTTGGSERVLSVKVAGIVTDSGVLSQSSGVLLVSQSYFNAAASGSPLLYD
                     TVDVTTADQSHTDLAVKDINAQFQLATTQTASDALKSQQGLVDNIKKFLEIAGLLALL
                     IGGVGIVNTMQVLLSRRKIEIAMLKTTGYRRVDLYLLFGLEAGLLGLIGGAVGALVAT
                     GISFLVRNLVQQTFGITIPFILDPITIGGGVLIGLLTALIFGLMPIVQAANIRPLNVI
                     RELPGSNRAGSFALTIVLLLLLSVLFCLLAVFILNDVVLGIGSVYGAFVFLGLLSLFF
                     GLVVFLVSILPVPERFTWKYLGLLVLGVAASVLIALALPTFGYLLLAVTLMGFVVVLL
                     PRGWKSSTKIALRNIGRQRARTTTTLLALFVGIFTIGLILILGQDLRDNINNAIASDL
                     TYNVITIASKDEVGALQGGLTTIPGLGKSEQNTIANTVPVAVNNQSINDLLKNVPSGF
                     SFTSLGKQGVLLFLNGVQGYDVAHNQVPDINTLTITSGRNLNASDAGQNNVLIAWQLA
                     NLDPLKGKIGVGSTITLASVDSKQVVTVHVVGVYRSSGINSSFKP"
     gene            complement(2178..2927)
                     /locus_tag="VGT44_22905"
     CDS             complement(2178..2927)
                     /locus_tag="VGT44_22905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV2583728.1"
                     /translation="MDETLREGLTALQESERRVVMDVRDITKSLPLGRERIDILRGIS
                     FQIASGEFVSIVGPSGSGKSTLLGIIAGLDNPTSGQVLIDGVDISSMSEGKLAEVRNN
                     KIGMVFQAFNLIPTLTAQENVEVPLYVGKHKGLPSTRARELLDLVGLSHRLTHRPNQL
                     SGGEQQRVAIARALATDPAFVLADEPTGNLDAKNSENVLKLIAYLRQQTGKTFIIATH
                     DPTVAANADRAIRIVDGQIASIETISRSDPQ"
     gene            3355..4743
                     /gene="secD"
                     /locus_tag="VGT44_22910"
     CDS             3355..4743
                     /gene="secD"
                     /locus_tag="VGT44_22910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEV2583729.1"
                     /translation="MRRGTLTLLLFIVLLGAAAVYIDLPNTPGIHILGINNPLNVKLG
                     LDLQGGVSVLLVPDPNQHYDSNTLNQEMPAVRDAIEQRVNGGLGVSEPSIRLQSSNNL
                     PAISVELPGFSGNQTQAINTLLQTGNLEFWSTGGSPAQIGAAFDPTQYTTYNPNNKAW
                     FTGKDLDPAQTYVSYDQAGRPQISFEMKGAAIGSFGSFTSKNVGQYLTVTLDRTVIES
                     AVIQNAITGPGVITGNFTLDSANQIVTVLKYGALPVVLQIKSEETIGATLGQDSIQKS
                     LIAALIGLSMVILFMLLYYRLPGLLADVALLLYACVTFAIFKLLGVVLSLAGIAGFVL
                     SIGMAVDANVLIFERVKEELRAGRLLSAAIDVGWKRAWPSIRDSNISTLITCAVLYGF
                     GNNFGASIIVGFATTLFLGVLISMFTAVVVTRTFLNLLVPTGVVSHPALFGLPPGSIA
                     TATLARRNSTVS"
     gene            4766..>5613
                     /locus_tag="VGT44_22915"
     CDS             4766..>5613
                     /locus_tag="VGT44_22915"
                     /inference="COORDINATES: protein motif:HMM:NF019187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583730.1"
                     /translation="MFNLVGKRYIFLGISLLIIIPGVLSLIFKGLNVGIDFSGGTSFE
                     FRPQSSFASIAAVQNTIKSFNFSELQVITGADTTVHGNQSVWVRLNTQVDTNVEKDIT
                     TDITGHYGANNVNVQFDDVLLNPLTGQGKATTVTVVTLAFQNVTPKIADIQSVLAHLP
                     DTSNPALGPATSTPTPTPTTAPTTTPTATTTPKVTPTVTTTPKATPTVTTTPKATTTP
                     TTTPTATATVTDTNPSNIKVSVEKVYQGTTTQTITLLTLTTLDQATVLKLKAALANNG
                     NYIYVL"
BASE COUNT         1188 a         1617 c         1663 g         1145 t
ORIGIN      
        1 gggcttgaag gaactgttga tgccgctgct gcgatacacg cccacgacgt gaacggtgac
       61 aacctgtttg ctatcgacgc ttgccagcgt aatcgtgctg ccgacgccga tcttgccctt
      121 gagcgggtcc aggttggcga gctgccaggc gatcaggacg ttgttctgcc cggcatcgct
      181 ggcattgagg ttgcgcccgc tggtgatggt gagcgtgttg atatcgggga cctggttgtg
      241 cgccacgtcg tagccctgca cgccgttgag gaagagcagc accccctgct tgcccaggct
      301 ggtgaaggaa aaaccggacg gcacattttt caggaggtcg ttgatggact ggttattgac
      361 ggccaccggc acggtattgg cgatggtgtt ctgctcggat ttgccgaggc caggaatggt
      421 cgtcaacccg ccttgcagcg cgcccacttc gtccttcgag gcaatcgtga tgacattgta
      481 ggtcaggtcg ctggcgatgg cgttgttgat gttgtcgcgt aaatcctgtc cgaggatcag
      541 gatcaggccg atggtgaaga tgccgacgaa gagcgccagc agcgtcgtcg ttgtgcgcgc
      601 gcgctggcgg ccaatgttgc gcagagctat cttcgtgctg gatttccaac cgcgcggcag
      661 cagcaccacc acaaagccca tcagtgtgac cgccagcagc aggtagccga aggtgggcag
      721 cgccagggca atcagaacgg aggccgccac gcccagcacg agcaggccca ggtatttcca
      781 ggtgaagcgt tcaggcaccg gcaggatgct gacgaggaag accaccagtc cgaagaacag
      841 gctgagcagc cccagaaaga cgaaggcgcc atagaccgag ccgatgccca gcacgacatc
      901 gttgaggatg aacacggcca gcaggcaaaa cagcacggag agcagcagta gcaagacaat
      961 cgtcagggcg aagctcccgg cgcggttgct gcccggcagc tcgcgaatga cgttgagcgg
     1021 gcgaatgttg gcggcctgca caatgggcat caggccaaag atgagggccg ttagcaggcc
     1081 gatcagcacg ccgccgccga tggtgatggg atcgaggatg aaggggatgg tgataccgaa
     1141 ggtctgctgc acgaggttgc gcaccagaaa gctgatgccg gtcgcaacca gcgcgccaac
     1201 cgcgccgccg atcaacccaa gcagtccagc ctccaggcca aagagcaggt agaggtcaac
     1261 gcggcggtag cccgtcgttt tcagcatagc gatttcgatt ttgcgccgcg agagtaagac
     1321 ctgcatggtg ttgacgatgc cgacgccgcc gatcaacaag gccagcagcc cggcgatctc
     1381 caggaatttc ttgatgttat cgacaagccc ctgctgcgat ttcagtgcgt cggacgccgt
     1441 ttgcgtcgtg gccagctgga attgcgcgtt gatgtccttg accgccaggt cggtatggct
     1501 ttgatcggcg gtcgtcacat cgacggtatc gtagaggagc ggcgagccgc tcgcggcggc
     1561 gttgaagtag gactgactga cgagcagaac gccgctggac tgcgacagca cgccagaatc
     1621 ggtgacgatg ccagccacct tcacgctcag aacgcgctcg ctgccgccgg tggttgagcc
     1681 gacatgcacg tcgaaggtat ctcctacctg cttatggaac tggtctgcca gggtcttatc
     1741 gatgatgacg ccattaccgg tcaacagcga tgcaatgctg ccgttcgagg gttcgacgaa
     1801 ggtgggcggc gtcacgatgg ggaaggtcgc ggcatcaacg gcctggatgg tgaaactgtc
     1861 cgtgatagag gccgagttgc ccatgctgcc ggtcgaattg atcacggcgg tatagtctgt
     1921 gatggttccc tggcttttga gctggctaaa gtagctcagg tcgtcgttgg tgaacggttt
     1981 ggtggtggag gtgacggcga tatccccgcc gttggcgtcg cgcacgttgc tggtcagcgc
     2041 gccgctgatc atcagtccga ccagttgcag cgccacaata gccatgacgc cgacagcgac
     2101 gcagaagatc gccagcacgg tgcgctgccc gccgcgcatc agcgagcggg tggtgtagtt
     2161 aaagtacatg gaggtcttca ttgtggatcg ctcctgctta tcgtctcgat cgaggcgatt
     2221 tgcccatcga cgatgcggat ggcgcggtcg gcgttggcgg ccaccgtcgg atcgtgcgtg
     2281 gcgatgatga aggttttgcc ggtctgctgg cgcaggtagg cgatcaattt cagcacgttt
     2341 tcgctgtttt tcgcgtccag gttgcccgtc ggctcgtcgg ccagcacaaa ggccgggtcg
     2401 gtcgccaggg cgcgcgcaat cgcgacgcgc tgctgctcgc cgccggagag ctggttgggc
     2461 cggtgggtca ggcgatgcga aaggccgacg aggtcgagca attccctggc gcgcgtcgat
     2521 ggcaggccct tgtgtttacc gacgtagagc ggcacctcga cattctcctg cgcggtcaag
     2581 gtgggaatca ggttgaaggc ctggaagacc atgccgattt tgttgttgcg cacctcggcc
     2641 agcttgccct cggacatgct gctgatgtcc acgccatcga tcaagacctg cccgctcgtt
     2701 ggattatcca gcccggcgat tatgcccagt agcgtgcttt tgcccgaacc ggaggggcca
     2761 acgatagaga cgaactcacc gctcgcaatc tggaagctga tgccgcgcag aatgtcgatg
     2821 cgctcgcggc ccagcggcag actttttgta atatcgcgca catccatcac cactcgtcgt
     2881 tcagattctt gcaatgcagt aagcccttcg cgtaatgttt catccatgtt tttcttcctc
     2941 ttattatatt ccgaattatt tcaacacaca ttgaaatatt aacgcagaag tccaatggtc
     3001 gtcaaatcga aacaatggag aggttgcgag cattctgtat tgtgaggcaa acatagctca
     3061 gagatttgga gatcgtgtct gtcatcctga gcgcagcgaa ggatctcacc gcccggcgag
     3121 gcagatcctt cgctgcgctc aggatgacag acaggacacc atataagtcc gatgaaggga
     3181 tgtcttctct tcaaacgtct aaaaatacca caggtgatat gcaacaagct tcaactatgc
     3241 tatactgcat aggaatttta tcgtctatcc caggctgacc ctggcgacaa cctgatatca
     3301 tgcatgattg agcgagctat cgtcactaca taaagagcaa ggggaactat acctatgcgt
     3361 cgaggaacat taacactcct cctgtttatt gtcctgctgg gagcggcggc ggtatacatc
     3421 gatctgccta atacgcctgg catccatatt ttgggtatca acaatccgct gaacgtcaag
     3481 ctgggactcg atctccaggg tggggtgagc gtgctgctgg tgccagatcc caaccagcac
     3541 tacgattcga atacgctgaa ccaggagatg cctgccgtgc gcgacgctat cgagcagcgc
     3601 gtcaacggtg gcctgggggt cagcgagccg agcatccgcc tgcagagcag caataacttg
     3661 ccggctatct cggtcgaact gccgggtttc agcggcaacc agacgcaagc catcaatacg
     3721 ctgctgcaaa ccggcaacct ggagttctgg agtaccggcg gttccccggc gcaaatcggc
     3781 gcagccttcg acccgaccca gtacacaaca tacaatccta acaacaaagc gtggttcact
     3841 ggtaaagatc tcgatccggc acagacctat gtcagctatg accaggccgg tcgcccgcag
     3901 atcagcttcg agatgaaggg cgcggccatc ggctcatttg ggtcctttac cagcaaaaat
     3961 gttgggcagt acctgacggt gacgctcgac cgcacggtca tcgaatcggc ggtgatccag
     4021 aacgccatta ccggccccgg tgtgattacc ggcaacttca cgcttgactc ggccaaccag
     4081 atcgtcacgg tgctgaaata tggcgcgctg ccggtggtct tgcagatcaa aagcgaagaa
     4141 accattggtg cgacgctcgg ccaggactcg atccagaaaa gcttgatcgc ggctttaatc
     4201 gggctgagca tggtgatcct cttcatgctg ctgtactatc gcctgccagg actgctggcc
     4261 gatgtagccc tgctgctcta tgcctgtgtg acctttgcca tcttcaagct gctcggtgtg
     4321 gtactctcac tggccggtat cgccggtttc gtcctctcga ttggtatggc ggtggacgcg
     4381 aatgtgctga tcttcgaacg tgtcaaggaa gaactgcgag ccgggcgctt gctgtccgcg
     4441 gccatcgatg tcgggtggaa acgcgcctgg ccttcgattc gcgactccaa catctccacg
     4501 ctaattacct gcgccgtact gtatggcttc ggcaataact ttggcgcgag catcatcgtt
     4561 ggctttgcga cgacgctctt cctcggcgtc ctgatcagta tgttcacggc ggtcgtggta
     4621 acgcgcacat tcctgaacct gctggttccc accggcgttg tcagtcatcc cgcactcttt
     4681 gggcttcctc ctggctcgat tgccacggcg accctggcgc ggcggaatag caccgtttca
     4741 taagatcgaa gaagaggaga aagccgtgtt caatcttgta ggcaaacgtt acatcttcct
     4801 gggcatctcg ctgctcatca tcatccctgg cgtactctcg ctgatcttca aagggctgaa
     4861 tgtcggtatc gacttctcag gtggcaccag cttcgagttc cgtccccaga gttctttcgc
     4921 gtcgattgcc gccgtacaaa atacaatcaa gtccttcaac ttctcagagt tgcaggtgat
     4981 taccggcgcg gatactaccg tacatggcaa ccagagcgtc tgggtgcgcc tgaatacgca
     5041 agtcgataca aacgttgaga aggatatcac gaccgacatc acgggtcatt atggcgcgaa
     5101 caatgtcaac gtccagtttg acgatgtgct gcttaatccc cttacaggac agggcaaggc
     5161 cacgaccgtg acggtggtca ctctcgcatt ccagaacgtc acgccgaaga tcgcggatat
     5221 ccagagcgtt ctcgctcacc tgccggatac ctccaatccg gcgcttggcc ccgccacgtc
     5281 aacaccgacg ccgacgccga cgactgctcc gacgaccacg cccacggcca caaccacgcc
     5341 gaaggtgacg cctactgtga caaccacgcc aaaggcgacg ccgacggtca cgaccacccc
     5401 gaaagcgaca accacgccaa ccacgacgcc gaccgccacg gccacggtga cggataccaa
     5461 cccgtccaat atcaaagtga gcgtggagaa ggtttaccag gggacgacga cgcagactat
     5521 cacgctgctg acgctgacga cactcgacca ggccacagtg ctgaagctca aggcggcgct
     5581 ggcaaacaat ggcaactaca tctacgtgct gga
//
LOCUS       DASZAK010001307         1712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_495056, whole genome shotgun sequence.
ACCESSION   DASZAK010001307 DASZAK010000000
VERSION     DASZAK010001307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495056"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VGT44_22920"
     CDS             <1..200
                     /locus_tag="VGT44_22920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583731.1"
                     /translation="ELSCHPFTIHDDPHLRRFSRHLLIAGQDRRHPHRQVLVASMGGG
                     EPGMTLLIVQKHQGTTAKNLA"
     gene            complement(232..585)
                     /locus_tag="VGT44_22925"
     CDS             complement(232..585)
                     /locus_tag="VGT44_22925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583732.1"
                     /translation="MSACTPCGDSRLAHLSARSRHMLVRTLLFLGAVGLDRTWDLRSY
                     TGDGLGVLTGRPRAYGYFHTERFLSQIAQANGADTFTDGLAQWTARLWNPPTAPTQEV
                     EHLYYIDGQMPPYLR"
     gene            complement(671..1003)
                     /locus_tag="VGT44_22930"
     CDS             complement(671..1003)
                     /locus_tag="VGT44_22930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583733.1"
                     /translation="MARGFCPSRSPGQSFDGLSEARARCVAVGKLLREDGRHGHPAKL
                     RTDERAWLETYCHTSPGTPSRVVQTAFQERFGLQISLGYLNQVRANLGLGSRTHEVEK
                     KTRFVPAQ"
     gene            complement(1143..1595)
                     /locus_tag="VGT44_22935"
     CDS             complement(1143..1595)
                     /locus_tag="VGT44_22935"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEV2583734.1"
                     /translation="MADQQHLAVLKQGVAAWNAWRRQHPEIPPDLREADLGDADLSGA
                     DLRHANLMRANLSRAYLSEASLNEANLSEAELTGANLREALLLRADLRQARFRGANLI
                     GAQLNGAQLSGADFRGADLSGGSLSRTGLHETDFSEANLSKVVITRKW"
BASE COUNT          310 a          535 c          497 g          370 t
ORIGIN      
        1 aggaactgag ctgccatccc ttcacgatcc acgatgatcc gcacctgcgc cgcttttccc
       61 gtcacctgct catagcgggt caggatcgca ggcaccccca ccgtcaagtg ctggtcgcct
      121 cgatgggtgg tggcgagccg gggatgaccc tgctcatcgt gcagaagcac cagggcacga
      181 cagccaagaa tcttgcctga gttcccaatc agcccgcgcg ggatcaacga gtcagcgtag
      241 atagggaggc atctgcccat cgatgtagta gagatgctcc acctcttgtg ttggagcggt
      301 tggtgggttc cacaggcgcg ctgtccactg tgcgagccca tccgtgaagg tgtccgctcc
      361 gttcgcttga gcgatctgtg agaggaagcg ttcggtgtgg aaatatccat aggcccgagg
      421 tctgcccgtg agcacgccca gcccatcacc cgtgtagctg cgcagatccc aggtgcgatc
      481 cagtccgacc gccccgagaa agagcagggt tcgcaccagc atgtgacgag accgcgctga
      541 aagatgtgcc aggcgcgaat cgccacaggg agtacacgca gacacggcgg tttccagagt
      601 ggagagcaga ccagacgcct cggctgccgc aaccaggagc agtccccctg caccctcttg
      661 ccactggggt tcactgggca gggacgaatc gcgttttttt ctccacctcg tgggtgcggc
      721 ttcccaggcc gaggtttgca cggacctggt tcaaatagcc aagactgatc tggaggccga
      781 aacgttcctg aaaggctgtt tgcactacac ggcttggagt gccgggcgac gtgtggcagt
      841 aggtttccag ccaggcccgc tcgtccgttc gcagcttggc agggtgcccg tgtcttccat
      901 cctcgcgcag cagctttccc acggctacgc acctggcgcg agcctccgat aggccgtcga
      961 acgactgacc tggagaccgg cttgggcaga agcctctcgc caccgatgac ctgtttgcat
     1021 aaaggcaatc atctgctctt tggccgcggg tcgctgctcc gcttccatcg ttctctcctt
     1081 gctctgctct cttcacgaaa cggctctcgt ctcctttctg gtgggaacgc ttgaggtggc
     1141 ggttaccact ttctggtgat gaccaccttg ctgaggttcg cctcgctgaa gtccgtctcg
     1201 tggagcccgg tccgactcag gctgcctcca ctgaggtcag ccccacgaaa atctgcgccg
     1261 ctcagttggg ccccattgag ctgcgctccg atcaaattgg cccctctgaa acgtgcctgt
     1321 ctgagatcag ctcgtaagag gagcgcctcc cgaagattgg ctccagtcag ctcggcttcg
     1381 gaaagattgg cctcgttcag actcgcctcg ctcagatagg cacggctcag attagcccgc
     1441 ataaggttgg catgtctcaa gtcggcccca ctgagatcag cgtcaccgag atcggcctca
     1501 cgcaaatcgg gggggatctc aggatgctgt cttctccatg cattccatgc cgcgactcct
     1561 tgcttgagaa cagcgaggtg ctgctgatct gccatcgtgt ttccctcctc ttcgagtgtt
     1621 gctctcatta tatggctctg gagggaactc ttcaaggacg cattgcctgg ctcgtgccca
     1681 agggcaacgt ggctatctca ttctagatct gt
//
LOCUS       DASZAK010001308         1935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_317062, whole genome shotgun sequence.
ACCESSION   DASZAK010001308 DASZAK010000000
VERSION     DASZAK010001308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317062"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..>1935
                     /locus_tag="VGT44_22940"
     CDS             201..>1935
                     /locus_tag="VGT44_22940"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2583735.1"
                     /translation="MSVDKHLFRHRQTDTYDFYEGEHKPARWKSDEQIDRYVPTHCCY
                     CGVQCGMYLKVAGGKVVGVEPREDFPLNHGMLCPKGTTAYQTVNHPDRLTYPLMRRAG
                     KASPLERASWDEVLDAIAGRFRAIQAEYDKDAVAIYSGSSMTNEKCYLMGKFARVALG
                     TRHTDYNGRLCMSSATGANERSLGIDRAANPISDILHADCIFVIGANVGECFPIVTSY
                     LWQARDRGCKLIVADPRQTPIARTADIFLPLRSGTDSALLNGMLHVVIKEGLVDEDFI
                     RERTVGWEETKAMVEQYPPERVAKVCGLRPEAIIEAARMYGNANAALLYTARGLEHQS
                     KGVDNGVTAVNLSLATGNFGRPGAGYGTLTGQGNGQGGREVGQKASQLPGCREIENPE
                     DRAYICSVWGIDEKDLPHAGYPATLMSPAMLRKEIRACFMICSNPMVSLPDQHTVEQA
                     LRGLDLFVVTDFFLSETAELADIVLPGNVWAEDEGTTTSLEGRVIKYNKAVDPPGEAR
                     LDWQIVCELARRLGKGQYFNFHSARDIFDELRLCTRGAKSDYWGITYEKIEQQNGVFW
                     PCPSEDHPGTPR"
BASE COUNT          442 a          521 c          560 g          412 t
ORIGIN      
        1 agtcaactgg aactgtggtc gaggaacaag cgacgaataa gctctggtgg caagatctct
       61 gtccgcagtg caagcgcgtg atgcgcgctc aagcgcacct tgctgcgctc ggccagggag
      121 ggaatcgatt cctatgaatg aactctggaa cgtgtttttg ccagtaatct cattgctgag
      181 actagagagc agagaaacag atgagtgtcg ataaacatct ttttcgccat cgtcaaaccg
      241 atacctacga cttctacgaa ggcgaacata agccagcccg ctggaagtcg gatgagcaaa
      301 ttgatcgcta tgtgccaact cattgctgct attgtggtgt tcagtgcggt atgtatctca
      361 aagtggccgg tggcaaagtg gttggcgtcg agcctcgcga agactttccg ttgaaccacg
      421 gcatgctttg ccccaaaggc acgacagcgt atcagacggt gaaccaccct gatcgcctga
      481 cctacccgtt gatgcggcgc gcaggcaaag caagccccct ggaacgggca agctgggacg
      541 aggtacttga tgccatcgcg ggacgctttc gcgccattca agccgagtat gacaaggacg
      601 ccgtcgctat ttatagtggc tcgtcgatga ccaacgaaaa atgctatttg atgggcaaat
      661 ttgctcgcgt tgccctgggg acgcgccaca ccgattacaa tggtcgtctc tgtatgtctt
      721 cggcgacagg agcaaacgaa cgctcgctcg ggatcgaccg cgctgccaat cctatcagtg
      781 acatactgca tgctgactgt atctttgtga ttggcgcgaa cgttggtgag tgtttcccta
      841 tcgtgaccag ctatctctgg caggcacgcg accgtggctg caaactgatc gtagctgatc
      901 ctcgtcagac acccattgcg cgtacggcag atattttcct ccccttacgt tcaggtaccg
      961 attccgcgct actcaatggc atgcttcacg tggtgataaa agagggattg gtggacgaag
     1021 attttattcg cgaacgcacc gttggttggg aagagacaaa ggcaatggtt gaacagtacc
     1081 cacccgagcg ggtagcgaaa gtctgcgggc tgcgccccga ggctatcatc gaggcggcac
     1141 gcatgtatgg aaatgctaac gccgcccttc tttacacggc gcgtggtttg gagcatcaga
     1201 gcaagggtgt cgataatggg gtaaccgctg tgaacctgtc actggcaacg ggcaattttg
     1261 gcagacccgg cgctggatat ggcacgctta ccggccaggg caacgggcaa ggcggtcgcg
     1321 aagtaggaca gaaagcctca cagttgcctg gctgccgcga gatcgaaaat cccgaggacc
     1381 gtgcgtacat ctgctcagtt tggggtatcg atgagaaaga cctgccacac gcgggctatc
     1441 ccgccacact catgagtccc gcgatgctgc gcaaggagat tcgcgcctgc ttcatgatct
     1501 gctcgaaccc catggtctcg ctgcccgacc agcataccgt tgagcaagcg ctgcggggat
     1561 tagacctgtt cgtagtaacc gactttttcc tctcagaaac tgctgaactc gcagatatcg
     1621 ttcttccagg aaatgtctgg gccgaggatg agggcacgac gacgagcctc gaaggccgcg
     1681 tgatcaaata caacaaggcg gttgaccctc caggtgaggc gcgtcttgac tggcaaattg
     1741 tgtgtgagtt ggcgcgacgc ctgggcaagg gtcagtactt caacttccac tctgcacgtg
     1801 acattttcga tgaactgcgc ctgtgtacca ggggagcgaa atcggactac tggggcatca
     1861 cctacgagaa aatcgagcag cagaacggcg tcttctggcc ctgtccatca gaggatcacc
     1921 cgggcacgcc gcgcc
//
LOCUS       DASZAK010001309         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_673919, whole genome shotgun sequence.
ACCESSION   DASZAK010001309 DASZAK010000000
VERSION     DASZAK010001309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673919"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2751
                     /locus_tag="VGT44_22945"
     CDS             <1..>2751
                     /locus_tag="VGT44_22945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEV2583736.1"
                     /translation="TDDVPEPRFDDLIELDLGTIEPSLAGPRRPQDRVPMQNLGKVFR
                     DAYADRFVPATVNHTTEDALIRLETESNDTGKDPVAMKEDSDKARSAEKRSGNGHNGH
                     LRDVVVTMGNAQAHMTDGSVAIAAITSCTNTSNPSVMIAAGLLAKRAVERGLSVSPTV
                     KTSLAPGSRAVFDYLNKAELVPYLEALRFHLVGFGCTTCIAGGTPVLFANGTARRIEQ
                     MARNGGAALFAPTADGRLGTATQAEMTVQGERECVSLVLQDGRALVCTPDHEILCADG
                     RWVRADQLVLGQDRVVVGLEAPLDEPGDDEAGYALHVGNLAFTMDTSYERQRTLAFAR
                     LLGHLLSDGSISLLGQGRMHVGQAMDREAVLNDIELLTGIRPAATRYDQRKWTIVLPK
                     PFTDAISTLPGVRTGRRIQQASALPAFVLEERCPVTVVREFLGGLFGADGHAPVLHRW
                     GKSEEEATLEPPAYSQSAIPEHVEALKQVMGDVTRLLARCGVKTNGANVYEYPTRRAT
                     STYPAAQDGIPRVEVRLELPDGLTFIERVGFRYCIDKSLRASAAAVYWRLVDQTHRQR
                     LWMSTRLQELHEADYKLSFTHARKKAAVELMEREAVVSPHYALLEGHDRFSRLPQVTA
                     RKFQPLHRDSCDFPSPVELLTEIGAREWFAPLRSRADAETSKRYCVEKEERTLPTLGL
                     QVVERLPAGRRAVFDLAVNDLHTFVAGTVAVHNCIGNSGPLPEPVAEAVQENDLVVAA
                     VLSGNRNFEGRIHPQVRASFLASPPLVVAYALAGTVDIDLAHDPLSYDSNGEAVYLRD
                     IWPSQAEINAVIDKSITPELFEQNYASVFEGDEHWRSLPNTEGELFNWDPDSTYIQEP
                     PFFQNMPTEAAPVKAIQGARVLVMVDDSITTDHISPAGNFGPTSPAGQFLQARGVERR
                     DF"
BASE COUNT          535 a          845 c          877 g          494 t
ORIGIN      
        1 gcaccgacga cgtgccggaa ccgcgcttcg atgacctgat cgaacttgac ctcggcacta
       61 tagagccaag cctggcggga ccgcgccgac cgcaggaccg cgtccccatg cagaacctgg
      121 gtaaggtctt tcgcgacgcc tatgcggatc gttttgtacc cgcgacggtc aaccatacca
      181 cagaagatgc gttgatccgc ctggaaacgg agagcaacga taccggcaaa gacccggttg
      241 ccatgaagga ggatagcgat aaagccagaa gcgcagagaa gcgcagcggt aatgggcaca
      301 acggccacct gcgcgacgtg gtcgtcacga tgggcaacgc gcaggcgcac atgaccgacg
      361 gctcggtcgc catcgccgcc atcaccagtt gcaccaatac ctccaatccc tcggtgatga
      421 tcgcggctgg cctgctggcg aagcgcgccg tcgaacgcgg cctctcggtc agcccgaccg
      481 tcaagaccag cctggccccc ggctcgcgcg cggtcttcga ctacctcaac aaggccgagc
      541 tggtgccata cctggaggcg ttgcgcttcc acctggtagg atttggctgt actacgtgta
      601 tcgccggagg aacgccggta ttgtttgcca acggcaccgc tcggcgcatc gagcagatgg
      661 cgaggaatgg aggcgctgca ctgttcgctc caaccgcaga cggcagactg ggtacagcga
      721 cgcaggctga gatgacggtt cagggcgagc gtgagtgcgt ctcactcgtt ctgcaagacg
      781 gacgcgcgtt ggtctgcaca ccggaccatg agatcctctg cgcggacggg cgctgggtgc
      841 gcgccgacca gctcgtactg ggccaggacc gcgtcgtggt tggtcttgag gctccgttgg
      901 acgagccagg tgacgacgaa gccggttatg cactacacgt gggcaacctg gcattcacga
      961 tggacacgtc atatgagcgc cagcgcacat tggcgttcgc acgcttgctc ggacatctgc
     1021 tgagcgatgg ctcgatcagt ctcctgggac agggccggat gcacgttggg caggccatgg
     1081 atcgcgaagc ggttctcaac gacattgagc tgcttaccgg cattcgtcct gcggcaacgc
     1141 gctacgacca gaggaagtgg accattgtgc tgccgaagcc gttcacggac gctatcagca
     1201 cgctgcctgg cgtgcgcaca gggcggcgca ttcagcaggc gtcggcgctc ccggcattcg
     1261 tgttagaaga gcgctgtcct gtcacggtgg tgcgcgagtt cctgggcggc cttttcggtg
     1321 cggatgggca tgcgcctgtc ttgcaccgtt ggggcaagag cgaggaggag gcgacacttg
     1381 agccgcctgc ctattcccaa agcgcaatac cagagcatgt ggaggcgctc aagcaggtta
     1441 tgggcgacgt gacccgtctg ctggcgcgct gtggggtgaa gaccaatgga gccaacgtct
     1501 acgaatatcc cacacgccgc gcaacttcca cgtacccggc agcacaggat ggtatccctc
     1561 gcgtggaggt tcgcctggag cttccagacg gcctcacgtt catcgagcgc gtgggattcc
     1621 gctattgcat agacaagtcc ctgcgagcca gtgcggcggc ggtgtattgg cgtttggtgg
     1681 atcagactca ccggcaacgc ctctggatgt cgactcgtct tcaggaattg cacgaggcag
     1741 actacaagct ctcgttcacc catgcgcgca agaaagcggc agtggaactg atggagcgcg
     1801 aagcagttgt ctccccgcac tacgcactat tggagggtca tgatcgcttc tcgcggctgc
     1861 cacaggtgac ggcgcgcaag ttccagccac tgcatagaga ttcctgtgac tttccttcgc
     1921 ccgtagaact cttgaccgaa atcggcgcgc gtgagtggtt tgccccgttg cgctcgcgag
     1981 cggatgcaga gacttcgaag cgctactgcg ttgagaagga ggagcgcaca ctgccgacac
     2041 tcgggctcca ggtagtggaa cgactgccag caggcagacg ggcggtgttc gacctggcag
     2101 tgaacgacct acataccttc gtggcaggca cggtagcggt ccacaattgc atcggcaata
     2161 gcggcccgct gccggagccg gtcgccgagg cggtgcagga gaacgacctg gtcgtggcgg
     2221 ctgtgctgag cggcaaccgc aacttcgaag ggcgcattca cccgcaggtg cgtgctagct
     2281 tcctggcctc acccccgctc gtggtggcct acgccctggc cggaactgtc gatatcgacc
     2341 tggcgcacga tcctttgagc tacgattcca acggcgaggc ggtctacctg cgcgatatct
     2401 ggccatcgca ggcggagatc aacgcggtca tcgacaaatc gatcacaccc gaactgttcg
     2461 agcagaacta cgcttctgtc ttcgagggcg atgaacactg gcgatccctg cccaataccg
     2521 agggcgagct attcaactgg gaccccgatt cgacctatat ccaggagccg cccttcttcc
     2581 agaacatgcc gacggaagct gcgccggtaa aggcgatcca gggcgcgcgc gtgctggtga
     2641 tggtggacga ctcgatcacc accgaccata tctccccggc tggcaacttt ggcccgacca
     2701 gtcccgccgg gcaattcctg caggcgcgtg gcgtggagcg gcgcgatttc a
//
LOCUS       DASZAK010001310         2358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_389726, whole genome shotgun sequence.
ACCESSION   DASZAK010001310 DASZAK010000000
VERSION     DASZAK010001310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389726"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..883
                     /locus_tag="VGT44_22950"
     CDS             38..883
                     /locus_tag="VGT44_22950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583737.1"
                     /translation="MRIRWILFIIVSACLIGGLVAGVYARSIEAALTTQPVASQGTGG
                     TQGKPPTVTQSPTTGQSRPSATPTTPPKATPTPATTPLPTGVSVLARDTFQRAAQVFW
                     GTASDGNMWVGDANSIEIFSITHDAGLIAAGQGAFNAILGPSSTDAEIVVSATVNQFA
                     ANGAVNLGVALRWKDGNNWYKALINGSQLQILSRVQGVTKVLAAMPFTAAGGTSYTLR
                     FRAMGANLLAKAWLTGQPEPANWLLQTTDTALTQGMGGIRVVLQNTTTIQVTSFLETT
                     VGIIG"
     gene            1058..>2358
                     /locus_tag="VGT44_22955"
     CDS             1058..>2358
                     /locus_tag="VGT44_22955"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEV2583738.1"
                     /translation="MIARFKAAYDAQRRVRLATLIGLGVAAIALLATGGFPPWAWRFL
                     FQVLSQLSRLWEVRGLAMLLPFVGLLMLALTQAILWCAIVLAAVYVVRDWLQRRRDMQ
                     AFELELAEAGRSVGTPFDAFAAPLAPPFAASAPVALASRQYEQQETRPVGRSSKARTT
                     PVAVVEATEDLEQSVVSLEIGSALDCGIKRKGQPNEDSLLALPNLRSSRKGIQPVALF
                     VVADGMGGHGNGQEASRLAIAALHDALRLALLDGLTDMASNPDMFYEELLAEGTHNAN
                     LAIYQRNRQKQTDMGTTLTAALIVGNTAYIANVGDSRTYLYRQDEGLSQLTRDHSAVA
                     RLVEVGAITPREVYSHPKRNEIYRSLGNSSSVKVDTYSVPLRIGDLLLLCSDGLWEMV
                     YDDDIAAIIASWMPTPAHICEALLQAALRGGGKDNISIIAVR"
BASE COUNT          478 a          723 c          716 g          441 t
ORIGIN      
        1 cagcatttga ggataattta taaaaagagg tgagcatatg cgtatacgct ggattttgtt
       61 tatcatcgtc agcgcctgtt tgatcggcgg gctggtcgcc ggtgtttatg cgcgttcaat
      121 tgaggcggcg ctgacgacgc agccggttgc ctctcaggga acgggaggaa cgcaggggaa
      181 gccgccgact gtgacgcaat cacccacaac cggccagtcc aggccaagcg cgacgccgac
      241 gacgccaccg aaggcgacgc cgacaccagc cactacgccg ttaccgaccg gcgtgagcgt
      301 actggcgcgc gacaccttcc agcgcgccgc ccaggtgttc tggggaaccg cctccgatgg
      361 gaatatgtgg gtcggcgacg ctaatagcat cgagatcttc tccatcacac acgatgccgg
      421 cctgatcgcc gctgggcagg gcgcattcaa cgccatcctc ggccccagca gcaccgatgc
      481 agagatcgtg gtcagcgcca ccgtcaacca gttcgcggca aacggcgccg tcaacctggg
      541 cgtggcgctg cgttggaaag acggcaacaa ctggtacaag gccctcatca acggcagcca
      601 gttgcaaata ctcagccgtg ttcaaggcgt cacgaaggtg cttgctgcta tgcctttcac
      661 cgccgccggc ggcacgtcgt acacgctgcg cttccgggcg atgggcgcga acctgctcgc
      721 aaaagcctgg ctaaccggcc agcccgaacc ggccaactgg cttttgcaaa ccaccgatac
      781 cgccctgacc cagggcatgg gaggcatccg cgttgtgtta caaaacacca ctaccatcca
      841 ggtcacatca ttcctggaga cgaccgtcgg tattatagga taatttgatg ttcgtaggtg
      901 ccgatttatc gcgcacaccg ccgattcatc ggccaagatg gggataatcg gcaatcaact
      961 aggccgatga atcggcggtg tgcgcgataa atcggcacct acggaaaatg caatacattg
     1021 ataattgccc tactatggat agaaggagag gtcagatatg atagctcgct tcaaagcagc
     1081 ttacgatgca caacgccggg tgcggctggc cacgctgatc ggcctgggcg tggccgcgat
     1141 tgcgcttttg gcgaccggcg gcttcccacc ctgggcctgg cgtttcctgt tccaggtcct
     1201 ttcgcagttg tcgcgtctgt gggaagtacg cgggctggct atgctcctgc cattcgtggg
     1261 actgcttatg ctggcattga cccaggcgat cctgtggtgc gctattgtcc tggcggcagt
     1321 gtacgtggtg cgggactggc tgcaaaggcg gcgagatatg caggcattcg agctagaatt
     1381 ggccgaggcc ggtcgctcgg ttggtacgcc gttcgacgcc tttgctgccc cgctcgcgcc
     1441 tcccttcgcg gcctctgctc ctgttgcgct tgcctcgcgc cagtatgaac agcaggaaac
     1501 gcggcccgtc ggacggagca gcaaagcccg cacaacgccc gttgcagttg tggaggccac
     1561 cgaagacctg gagcaaagcg tcgtctcgct ggagatcggc tcggcgctgg attgcggcat
     1621 caaacgcaag ggacaaccca atgaagatag tctgctggcg ctgcccaacc tgcgatcatc
     1681 gcgcaagggt atacagccgg tggcgctctt tgtcgtggcg gatggcatgg gcggacatgg
     1741 caacgggcag gaggcaagcc gattggcgat tgccgctctg cacgacgcgc tgcgactcgc
     1801 gctgctagat ggtctgaccg atatggcaag caacccggat atgttctatg aagagctgct
     1861 ggccgaaggc acgcacaacg ccaacctggc catttaccag cgcaatcggc agaagcagac
     1921 cgacatgggt acgacgctca ccgccgccct catcgttggg aacaccgcct atatcgccaa
     1981 cgtgggcgac agccgcacct acctgtatcg ccaggacgaa gggctttcac agctcacacg
     2041 cgaccactcg gctgtcgcgc ggctggtcga agtgggcgct atcacgccgc gcgaggtcta
     2101 ctcgcacccc aagcgcaacg agatttaccg cagcctgggc aactcatcca gcgtcaaagt
     2161 cgatacatac agcgtgccgc tcaggatcgg cgacctgctg ctgctctgtt ccgatggcct
     2221 gtgggagatg gtgtacgacg acgatatcgc cgctatcatc gcatcgtgga tgccaacccc
     2281 cgcgcatatc tgcgaggctc tactgcaagc cgcgctgcgc gggggcggca aggataatat
     2341 cagcatcatc gccgtccg
//
LOCUS       DASZAK010001311         1853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_209620, whole genome shotgun sequence.
ACCESSION   DASZAK010001311 DASZAK010000000
VERSION     DASZAK010001311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209620"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /gene="deoA"
                     /locus_tag="VGT44_22960"
     CDS             <1..356
                     /gene="deoA"
                     /locus_tag="VGT44_22960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907973.1"
                     /note="Catalyzes the reversible phosphorolysis of
                     thymidine, deoxyuridine and their analogues to their
                     respective bases and 2-deoxyribose 1-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV2583739.1"
                     /translation="DARQIRDPELLPAAPARLMLTAPRSGYIAAIAAEQVGLASMRLG
                     AGRFKKGEQIDHRTGFILQAKVGDYLHVGDPLVEIHARSDSEADSVRDTLLGSYQWSD
                     SPVKAGALVYETIRP"
     gene            complement(361..>1853)
                     /locus_tag="VGT44_22965"
     CDS             complement(361..>1853)
                     /locus_tag="VGT44_22965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583740.1"
                     /translation="IEERRTYHRHAVLADDNAPGSLAKKPLTPAASPMPPATPAHDPI
                     AFPDSRHAQHASSYAPARPTRTPLPQRSRKDLEQEALAMQLASLEDSLQQRIVAHPAP
                     LSRAVGMTKAGGMRGTLGRMKSITASKRLTRRFVQIVIVMLFLFTGALASFFALNYYA
                     HQSKQKLPQQQPTITQPPTIAAQVSWQALPSLPSSQADNTASYVVIQGHAYVYVSGGY
                     RGATAHPSYVRGLYRYDIAAALWENVSVPGFPTMGNNAAAVDEHQRIFFTGGYSADAH
                     AVVSALYMYNPQSNKLQKITPPSQVHLGFGNSMIADQEGHLYVTQGFTTPGNPHALAG
                     TGWYRYDIANGQWEVLAALPVGAGYVLLAPDGQGSILMIGGSEDAGQHMPVAQIYRYD
                     TGHNSWSLAPSASPTDLSGAAGCLDSQGHLIIIGGYDAQHATLLDTVWQMTLATLSWQ
                     QLPALPSGSSLLGAAACAGNGRVYLERGASTGESPTADFLELANGM"
BASE COUNT          365 a          539 c          590 g          359 t
ORIGIN      
        1 gagatgcccg gcagatccga gaccccgaac tactacccgc cgcgcccgcg cgcctgatgc
       61 taacagcgcc gcgttcgggc tacatcgccg ccatcgccgc ggaacaggtc ggcctggcca
      121 gtatgcgcct gggcgcgggc cgcttcaaga agggcgaaca gatcgaccac cgcaccggat
      181 tcatcttgca ggccaaagtg ggagactacc tccacgtcgg tgatccatta gtggagattc
      241 acgcgcgcag cgactccgag gccgacagtg tgcgcgatac gctgctgggg agctatcagt
      301 ggagcgattc gcctgtgaag gcgggagcat tagtgtacga gacgatccgt ccataaattg
      361 tcacatcccg ttagccagtt ccaaaaaatc cgccgtcggg gactcgcctg tgctcgcccc
      421 gcgttccagg tacacacgcc catttccggc gcaggctgcc gcgccgagca gggagctgcc
      481 ggagggcaaa gcggggagtt gctgccagct cagcgtggca agcgtcattt gccagacggt
      541 atcaagcaag gtcgcgtgct gtgcatcgta gccgccgatg atgatgaggt ggccctgact
      601 atccaggcag ccagccgcgc cgctcaagtc ggttggcgag gcagacggcg caaggctcca
      661 gctattgtga ccggtgtcgt agcggtagat ttgcgcgaca ggcatatgct ggccggcatc
      721 ctctgaacca ccgatcatca gaatactccc ctgtccatcg ggggccagca gcacataccc
      781 cgcgccgacg gggagcgcgg ccagcacctc ccattgccca ttggcgatat catagcgata
      841 ccagccggta cccgccaggg cgtgcgggtt gccaggcgtg gtaaagccct gcgtgacata
      901 gagatggcct tcttgatcgg cgatcatgct gttgccaaag ccgagatgga cctgcgaggg
      961 tggcgtaatc ttttgcagtt tattgctctg cgggttatac atgtagagcg ccgataccac
     1021 cgcgtgcgca tcagccgagt aaccgccggt gaagaagatg cgctggtgtt catcgacggc
     1081 tgccgcgtta ttacccatgg tcggaaaacc tggtacactc acgttttccc agagggcggc
     1141 tgcgatgtca taacgataga gaccgcgcac ataggatggg tgggccgttg caccccggta
     1201 gccaccgctc acatagacat aggcatggcc ctggatcacc acatagcttg cggtattatc
     1261 agcctgtgaa gaaggcaagg atggaagcgc ctgccaggaa acctgggctg caattgtggg
     1321 cggctgtgta attgtcggct gctgctgagg cagcttttgc ttcgactgat gcgcgtagta
     1381 gttcagcgca aaaaatgagg cgagcgcgcc ggtgaagaga aagagcatca caatcacgat
     1441 ttgaacaaag cggcgcgtta atcgcttgct tgcagtgatg ctcttcattc tccccagggt
     1501 tcctcgcata ccgcccgctt ttgtcatacc cacagcccgc gagaggggtg cgggatgcgc
     1561 gacgatacgc tgctgcaagc tgtcctccag cgatgccagc tgcattgcca gcgcctcctg
     1621 ttccaggtct ttgcggctcc tctgcggaag aggcgtgcgt gtcgggcgcg cgggcgcgta
     1681 agacgaggcg tgttgagcat gtctggagtc tggaaaagcg attggatcgt gtgccggtgt
     1741 cgcaggaggc atcggagatg ctgctggcgt tagcggtttc ttcgccaacg agccgggcgc
     1801 attatcgtcg gccagcacag catgacggtg gtaggtcctg cgttcctcga tgg
//
LOCUS       DASZAK010001312         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_175769, whole genome shotgun sequence.
ACCESSION   DASZAK010001312 DASZAK010000000
VERSION     DASZAK010001312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175769"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /locus_tag="VGT44_22970"
     CDS             <1..1119
                     /locus_tag="VGT44_22970"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2583741.1"
                     /translation="LMAPPLVARQVERPPRRISRRGLLIGGTALGAALVGLIGTFEVL
                     DTRAFSRMAMTAQPEKVPSFKGNSLIFRGHTDSVISVALSPDGSKIASASADKTVQII
                     DATSGSPVLTYTGHSAGVTGVGWSPDGSSIVSTGLDSTVQVWDAATGQRLHLLGPQPG
                     QTINMAGPIYGGAWSTEVHRIATAVNGNLNLISTDAWESLGGGTGPIVMAPNALSWAP
                     HDPIIAVAEQDHSVYTWGINTEIGGASCVGHTRKVLAVDWSPDGSKLVSGGQDNTVRV
                     WSIPPMNQSATLSGSSLLVYQGHKDWVQAVAWSPDGSKIASAGKDTTVQVWDAATGEM
                     LYTYTGHSAPVNSLAWLLDGSSIISASDDGTVQVWSTM"
     gene            complement(1132..2481)
                     /locus_tag="VGT44_22975"
     CDS             complement(1132..2481)
                     /locus_tag="VGT44_22975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEV2583742.1"
                     /translation="METNQPTAILTHKDAVHEHLSAPATPATPTASTTPGRLPRSEIT
                     TLIIGWILLGGVLLSAAVILAGLALLFIQHVGLSAQALQAFPQTLGQVWSGVVTLQPQ
                     AIITLGLLLLIATPVMRVAVSIVAFALEKDRRYIVITTIVLTILLVSIFVVGSLVAGT
                     ASNAVLQVHFSMLVLALIFVASIVAGLLGSLVGLGGGVLIVPLLTLAFGFPISLAIGA
                     SIISVIATSSGAAAAYVRDHLTNLRVGMVLEIATTTGAITGAFLAGLLAPQLLFIIFG
                     VILLISAAPLVVKIGEELPKDVKNDKWANWLRLSGSYPDQHLGRVVPYQVTRTPLGAG
                     MMYIAGLISGLLGIGSGTFKVIAMDTLMRLPMKVSTTTSNFMIGVTAAASAGIYFSRG
                     DIPPLVAAPVALGILTGALIGARLLVHLSNKTLRLIFLPVIILAALEMVLHGFGIGI"
     gene            2643..>2886
                     /locus_tag="VGT44_22980"
     CDS             2643..>2886
                     /locus_tag="VGT44_22980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583743.1"
                     /translation="MTTITAIKRNIIDGHKLPACCVIHCREHVELYAIQPIERMAISM
                     VQLPRIKLSQRSLKRLLFGGLSGSVGLAGVFLAVSYY"
BASE COUNT          634 a          798 c          882 g          572 t
ORIGIN      
        1 ctcatggcgc cacccctggt ggcccggcag gtggagcgcc cgccccggcg tatttcgcgg
       61 cgcgggcttc tgataggcgg aacggcgcta ggagcggcac ttgttggatt gataggcacc
      121 ttcgaggtac tcgatactcg cgccttttcc aggatggcta tgactgccca gcccgaaaaa
      181 gtcccgagtt tcaagggcaa cagcttaatc tttagaggcc atacggattc tgtgatatcg
      241 gtggccctgt cgcccgacgg cagcaagatc gcctccgcga gcgccgacaa gaccgtacaa
      301 atcatcgacg ccacaagcgg ctcgcccgtc ctgacctata ccgggcatag cgccggagtc
      361 acgggcgttg gctggtcgcc cgatggcagt tccatcgtct cgaccggcct ggatagcacc
      421 gtgcaagtgt gggatgctgc gaccgggcaa cgcttacacc tgcttggtcc tcaaccgggt
      481 cagacaatca acatggctgg tccgatttac ggcggcgcct ggtcaacaga agtgcatcgt
      541 attgccactg ccgtcaatgg caaccttaac ctcatcagca ccgatgcctg ggaaagtctg
      601 ggagggggta caggcccaat tgtcatggcc cccaatgcgc taagctgggc gcctcatgac
      661 ccgatcattg ccgttgctga gcaggaccac agcgtttaca cctggggtat caatacggag
      721 attggcggcg catcctgtgt cggtcatacg cgcaaggtcc tggcggtcga ttggtcgccc
      781 gatggtagta agcttgtctc cggcgggcag gacaacacgg tgcgggtctg gagtatccct
      841 cccatgaacc agtcagcaac gctcagcggg agcagcctgc tcgtgtacca gggacataag
      901 gattgggtgc aggcggtggc ctggtcgccc gatggcagca aaatcgcctc ggctggcaag
      961 gatacaaccg tccaggtgtg ggacgcggcc accggcgaaa tgctgtacac ctatactggc
     1021 cacagcgccc cggtgaattc gctggcctgg ttgctggatg ggtcgtcgat catctcagcc
     1081 agcgatgacg ggaccgtcca ggtgtggagt acgatgtaga ggccccatgc attaaattcc
     1141 gatgccgaaa ccgtgcagca ccatctctag cgcggccagg ataattaccg gcaggaagat
     1201 caggcgcagc gttttattgc tcaggtgaac gagcaggcgc gcgccgatga gcgcgccggt
     1261 caggatgccc agcgccacag gcgcggctac caggggcggg atgtcgccgc gcgaaaagta
     1321 gatgcccgcg ctggcggctg ccgtgacgcc gatcataaaa ttgctagtgg tggtcgacac
     1381 cttcatcggc agacgcatca gcgtatccat cgcgatgacc ttgaacgtgc cgctgccgat
     1441 gcccagcagg ccagagatga ggccggcaat gtacatcatg cccgcgccga gtggggtgcg
     1501 cgtgacctgg taagggacca cgcggcccag gtgctgatcg ggataggagc cggaaagccg
     1561 cagccagttc gcccacttat cgtttttaac gtcctttggc agctcctcgc cgattttcac
     1621 gaccaggggc gcggcagaga tgagcaggat cacgccgaag atgatgaaga gcaattgtgg
     1681 agcgagcaga ccggccagaa acgcgccggt gatcgcgcca gtggtcgttg cgatttccag
     1741 caccataccg acgcgcaggt tggtgaggtg gtcgcgcaca taggcggcag cagccccgct
     1801 ggatgtggcg atgacggaga tgatgctggc cccgatggcc aacgagatcg ggaagccaaa
     1861 ggccagcgtc agcagtggaa cgatcagcac gccgcccccc agcccgacca gcgagccgag
     1921 cagacccgcg acgattgagg caacgaagat gagcgccagt accagcatag agaagtgtac
     1981 ttgcagaacg gcattgctgg ccgtgccggc taccaggctg ccgacaacga agatgctgac
     2041 aagcaggata gtcagcacga tcgtagtaat cacaatgtag cggcgatcct tttcaagcgc
     2101 aaaggcgacg atggagacgg caacgcgcat caccggcgtg gcgatcagca gcagcaagcc
     2161 cagtgtgatg atcgcctgcg gttgcagcgt cacaacgccg gaccagacct ggcccaatgt
     2221 ctgcggaaac gcttgcagcg cctgcgcgct gagtccgaca tgctggatga acagcagcgc
     2281 cagacctgcg agtatgacgg ctgcgcttaa caggacgccg cccagcaaaa tccagccgat
     2341 aatcagggta gtgatctcag acctgggcag tcgccctggc gttgtcgatg ctgttggtgt
     2401 tgccggtgtt gccggtgctg aaagatgctc gtgtacagcg tctttatgag taaggattgc
     2461 ggtcggttga ttcgtttcca taatgtgttg tcgtcgtcct cttacaaacc ggatcatgat
     2521 gtgttctgat aagctctgag acatctagat atctctacta ctattgtacg cgcacttctg
     2581 ttataattcc aatacatgtt tcttatcata tatataacta aaagttatga taagagcagg
     2641 gaatgacaac gattacagca atcaaacgta atataattga tgggcataaa ttgcctgcct
     2701 gctgcgttat acattgtaga gagcatgttg aattgtatgc cattcaacca atagaaagga
     2761 tggcgatttc aatggtgcag ttgccacgca tcaagttgtc tcagcgctcg ctgaaacggc
     2821 tcttgttcgg tggattgagt ggaagtgtcg gactggccgg tgtgttttta gcggtctcat
     2881 actata
//
LOCUS       DASZAK010001313         3155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_531949, whole genome shotgun sequence.
ACCESSION   DASZAK010001313 DASZAK010000000
VERSION     DASZAK010001313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531949"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="VGT44_22985"
     CDS             complement(<1..1446)
                     /locus_tag="VGT44_22985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEV2583744.1"
                     /translation="MEAMHDAIINPDFLAIGLGGTNMMSMLWTVAMGRQAVGVEMRGD
                     PFLGVHWNIREDFYHQLGLIDQLMLERYGEERLPRRGDGRLFRLAECFYSPDTVAGDI
                     VADEIIDGYDSDQHIVGTIHNVEFIDDRWRDGLPNRVITVLDPPTPPDRPDPRKIRTS
                     MVEVLDGPSTFQAEAASIQKLLRRYLELIEQLDLDNNLKPRVRLFTHHRVVPADGDGF
                     IDCPDGRKQIRIEALQEFDFKGQFVRVREPGSEVIDLGVPELFMIAEGFNSTDAKRLG
                     FEQQDVKVDHGDGHGPVVAQADFLAGLVEVLVGGRLRRRISSAFDQDGKEYWVRQIAV
                     GHENDPEVGWVLVQVPDFKTFDPIESGLLPAGTDPSSPEFFASYQQLIYDYYIKQAAE
                     ILEIPRQELMKVQMIYGPTLFSLVERVGADALIAANGVVAGDSFGNGHFLTSGGAMTG
                     MIGHSARVLEYWQSRDAGTSPADAIRRLADSI"
     gene            complement(1486..2754)
                     /locus_tag="VGT44_22990"
     CDS             complement(1486..2754)
                     /locus_tag="VGT44_22990"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV2583745.1"
                     /translation="MLTGQIFIQLIVILLAVQVFGYLCQCVGQPRVIGEILAGLALGP
                     TLLGAILPRVEAAIFPGSALPTLQVLGDIGLVLYMFSLGTHIDTHAMLKQGRKAAVVS
                     LSGVLLPLAMGGAFAFFLYPQFAGSKANLVSFMLLVGTAMAITAFPVLARLLEERGML
                     ATRIGVLALLCAAIDDVVGWCLLALVIAFIHATVAASVAITIALLGLFVGVMLCVVRP
                     LLFFADRHLKSKPALIGLTMILLLASAYATSAIGIHPVFGAFMMGIIMPRRTTYIEQV
                     KHIDQVNNLLFLPLYFVSNGLRTHIGLINSPALWLLCMLVLIVACLSKGLGGSLSLKV
                     FGESWKESLTLGTLMNTRGLVELIVLNIGLDLGVISPRFFAMLVIMAVVTTMMAPPLL
                     SFFGYRQKHVREQEAKETDPTNYKGLLTNK"
BASE COUNT          762 a          914 c          820 g          659 t
ORIGIN      
        1 gatgctatcg gcgagtcggc ggatagcatc agccggtgag gtgcccgcat cccgcgactg
       61 ccaatattcg aggacgcgag cactgtgccc aatcatcccc gtcatcgcac caccactggt
      121 caggaagtga ccattgccga aggaatcgcc agcgaccacg ccgttagccg cgatcagcgc
      181 gtcggctccg acgcgctcga cgagactgaa caacgtcggc ccatagatca tctgcacctt
      241 catcagttct tgcctgggaa tttcaaggat ctctgcggcc tgcttgatat agtaatcgta
      301 gatcaattgt tggtaggagg caaagaactc aggagaactg ggatcagtcc ctgctggaag
      361 cagtcctgac tcgatgggat cgaaggtttt gaagtctgga acctgaacga gaacccaacc
      421 aacctctgga tcattctcat ggcctacagc gatttgccgc acccagtatt ccttgccatc
      481 ctggtcgaac gctgaggaga tgcgccgccg cagacgtcca ccaacgagta cttcgaccag
      541 gccagccagg aagtccgctt gcgccaccac cggcccatgc ccgtctccat gatcgacctt
      601 gacgtcctgc tgctcgaatc ccaggcgttt cgcgtctgta ctattgaacc cctcggctat
      661 catgaacagc tcgggtacac cgaggtcaat gacctcactg cccggttccc gcacacggac
      721 gaactgcccc ttgaagtcga attcctggag ggcctcgatg cgaatctgct tgcgaccatc
      781 gggacagtca atgaaaccat cgccatcagc cgggaccaca cggtggtgag tgaacagccg
      841 gaccctgggt ttgagattgt tgtccaggtc caactgctcg atcagttcca ggtagcggcg
      901 cagcaatttc tggatggagg cagcctccgc ctggaacgtg gacgggccat ctagcacctc
      961 caccattgag gtgcgaatct tgcgaggatc aggccgatca ggtggcgttg gagggtccaa
     1021 tactgtaata acccggttcg ggaggccgtc ccgccaccgg tcgtcaatga attcaacgtt
     1081 gtgaatggtt cctacaatat gctggtctga atcgtagccg tcgatgatct catcggccac
     1141 gatatctccg gcgacggtgt ccgggctgta gaaacactcc gcgaggcgaa agagccgccc
     1201 atctccccgg cgtggcagcc gctcctcgcc gtagcgttcg agcatcaact ggtcgatcag
     1261 gccaagttgg tgatagaagt cttcacggat gttccaatgc acgccgagaa atgggtcgcc
     1321 gcgcatctcg acgccaaccg cttggcggcc catcgccacg gtccacaaca tagacatcat
     1381 attcgtcccg cccagaccta tggccaggaa gtctggattg attatggcat catgcatagc
     1441 ttccattagt agcgtgccct cctcatgagt gagcatacgt tgaggttatt tatttgtcaa
     1501 taatccttta tagttggtcg ggtctgtttc ttttgcttct tgctctcgca catgcttctg
     1561 tcggtatcca aagaaggaga gcagtggagg tgccatcatc gttgtgacga ccgccattat
     1621 caccagcatc gcgaagaacc tgggtgaaat cacgcccaga tccagtccga tgttgagcac
     1681 gatcaactcg accagccctc gtgtattcat cagcgtaccc agcgtgagcg actctttcca
     1741 ggactctccg aatactttga gggaaagtga ccctcccagc cccttgctga ggcaggcgac
     1801 aatcagtacc agcatacaga ggagccataa ggcaggcgag ttgatcagcc cgatgtgcgt
     1861 tcgcagcccg ttagatacga agtagagggg caggaacagc agattgttga cctggtcgat
     1921 gtgcttgacc tgctcaatat acgttgttct gcgcggcatg ataatgccca tcataaatgc
     1981 gccaaacacc ggatgtatcc ctatcgcact ggttgcatag gcagatgcca gcagtaaaat
     2041 catcgtgagc cctataagtg ctggcttcga tttcaggtgc cggtcggcaa agaacagcag
     2101 cggccgtact acacagagca tgaccccgac aaaaagccca agaagcgcta tcgtaatcgc
     2161 cacggaagct gcgactgtgg catgtatgaa tgcaatcact aatgccaaca ggcaccaacc
     2221 cacgacatca tcaatagcgg cgcagagcag cgccagcact ccaattctag ttgccagcat
     2281 gcccctctcc tcgaggagac gcgcaagcac cggaaatgct gtaatagcca tcgccgtacc
     2341 aaccagcagc atgaaagaca ccaggttggc ctttgatccg gcaaactggg gatacaggaa
     2401 gaacgcgaat gcacctccca ttgcaagtgg caggaggaca ccactcaggg agacgactgc
     2461 ggctttacgg ccctgcttca gcatcgcgtg cgtatcgata tgcgttccca gggagaacat
     2521 gtagagcacc aggccgatat cgcccaggac ctgcagggtt ggcaaggcgc tgccgggaaa
     2581 tatggctgct tcgactcgcg gcaagattgc tcccagcagc gttggaccca gcgcaaggcc
     2641 agccaggatt tccccgatca cccgcggttg gccaacacat tgacaaagat agccgaacac
     2701 ctgcaccgcg agcaaaataa cgatcagctg tatgaaaatt tgaccggtga gcatagcgtc
     2761 ctccctcatc gaatcaactg cttcaatatc ctgagcagat cacttcctcg tgagatggtc
     2821 tgctctacct tgatattacc ctgatatcac ctgggaagat agagcacagt tctctttcag
     2881 aatatgaaat gggtaactgt tgaagaagga tcaataatcc ttcgcgcccc ttgagtaaaa
     2941 caaggaggaa cgctaactca ccatgatgaa cgatgcagaa ggcgatctag cgcgtctcca
     3001 gaggacgagg cggaaaagct atatgtcagc gcggctgcat gcgtatcccg ccgtccatcc
     3061 gtcgctaact acctctccat aaacctcact cttttacaag caatgacaca ggaacttatc
     3121 caactttttg gtctcaagct tatcgtatca ctcag
//
LOCUS       DASZAK010001314         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_317215, whole genome shotgun sequence.
ACCESSION   DASZAK010001314 DASZAK010000000
VERSION     DASZAK010001314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317215"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..1084)
                     /gene="plsX"
                     /locus_tag="VGT44_22995"
     CDS             complement(41..1084)
                     /gene="plsX"
                     /locus_tag="VGT44_22995"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV2583746.1"
                     /translation="MSETARTVRVALDVMGGDNAPGETVAGAVQAARDYGIGVYLVGH
                     EDAIRAELAKHDTAGLDLPIVHTDEVIPMDEHDPAAAARSKKQSSMVLALQLVKDGAA
                     VGAVSAGNSGAMMAASLFTLRRLRGVERPALGGIMPTRTGATLVIDMGANTDCKPEYL
                     VQFGLMGSIYMERIFSISSPRVGLLSNGEEEGKGNAQVQEAHQMLKEAATTLGINFIG
                     NVEGRDIPAGNADVVVCDGFVGNVVLKLSEGLAESLIGLLRQQMTSNLPSKLAAAVLR
                     PGLRKVFGRLDYAEYGGVPLLGVNGSAIIAHGRSNAKAIKNALRVARQTAETNVAGAI
                     ADGLSKLGSPSEI"
     gene            complement(1081..1464)
                     /locus_tag="VGT44_23000"
     CDS             complement(1081..1464)
                     /locus_tag="VGT44_23000"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2583747.1"
                     /translation="MAKKILVMDDDPTIADLLREALADEGYETYMTTQSLRFYDALRE
                     HKPDLILLDLMMQYLDGRDELKLMEMADHKAPVIVVTAFPDAIKAEEEFRQAGVVHIV
                     YKPFDLEKLLDLVRATIGEPEGNRV"
     gene            complement(1612..2259)
                     /locus_tag="VGT44_23005"
     CDS             complement(1612..2259)
                     /locus_tag="VGT44_23005"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV2583748.1"
                     /translation="MTQTSRKNGSKPANGKIKRVRLTQKAIVAAAAELFARDGFGATS
                     LDDIASVLGVTKGALYYHVRNKEEILRLIYLTVLTASEEPLRKIVEEDLPPGEKLRRA
                     VEHHVVVAADRSPAMTVFYREHPHLTGPFSKEILLRKKAYERFFEQIIEEGQAAGVFH
                     ADIDHKIATFGLLGMCNWLSQWYRAEGQYTPQQIADMFMRMIGEGMFQRREILLP"
BASE COUNT          492 a          782 c          641 g          542 t
ORIGIN      
        1 ccgaaccgcc tgcggcggtt cgggagccgc ttcttgtcgc ttaaatctca gatggagaac
       61 ctagttttga gaggccatcg gcgatggctc cggcgacgtt cgtttccgcc gtctgccgcg
      121 cgacacgcag agcattcttg atagctttcg cattcgagcg gccatgcgcg ataatggccg
      181 aaccgtttac gcccagcaag ggaacgccgc catattcagc gtagtcgagt cgcccgaaga
      241 ccttgcgcag tccgggacgc aagacagccg ctgccagctt gctcggcaga ttgctggtca
      301 tctgctggcg cagcagcccg atcagcgact cggccaggcc ctcgctcaat ttcagcacta
      361 cattgcccac aaatccatcg cagaccacca cgtcggcgtt gcccgccggg atatcgcgcc
      421 cctcgacatt gccgataaaa tttatgccga gcgtcgtagc ggcctctttg agcatctggt
      481 gcgcctcctg tacctgcgcg ttgcccttgc cctcttcttc gccattcgac aacaggccaa
      541 cgcgcggcga ggagatcgaa aagatgcgct ccatgtagat cgatcccatc aggccgaact
      601 gcacaagata ctccggcttg cagtcggtat tcgcgcccat gtcgatcacc agggttgcgc
      661 ccgtccgcgt tggcataatg ccgcccagcg cgggccgttc aacgcccctc aagcgcctga
      721 gggtaaaaag cgacgccgcc atcatcgcgc cgctattgcc tgccgagacc gctccgaccg
      781 ccgcgccatc cttcacaagc tgcagcgcca gtaccatcga cgattgcttc ttgctgcgcg
      841 cggctgcggc ggggtcgtgt tcatccatcg ggataacctc gtcggtatgc acaatgggca
      901 aatcaagccc cgccgtgtca tgtttcgcca gttccgcgcg aatggcatcc tcgtgaccca
      961 ccaggtagac gccgatgcca tagtcgcgcg ccgcctgcac cgcgcccgcg accgtctcgc
     1021 cgggggcgtt gtcgccgccc atgacatcca gcgcgacgcg cacagtccgc gcggtctcgc
     1081 tcatacgcga ttcccttccg gctcgccaat cgtggctctg accaggtcca acaacttctc
     1141 caggtcgaac ggtttataga cgatatgcac cacgcccgct tgacgaaact cttcttccgc
     1201 tttgatcgcg tccgggaagg ccgtcacaac aatcaccggc gctttgtggt cggccatctc
     1261 catcaacttg agttcgtcgc ggccatccag atactgcatc atcaggtcaa gcaggatcag
     1321 gtccggctta tgttcgcgca gcgcatcgta gaaacgcagg ctctgcgtcg tcatgtaggt
     1381 ctcgtagcct tcgtcggcca gcgcctctcg caagaggtca gcaatagtcg ggtcatcgtc
     1441 catgaccagg atttttttcg ccatcagcgc cccctcgtgt aacaatggat tggaatgcgt
     1501 tttgcttcct cggatgttcc tattataaca tcacgcctga tactatggac gccgttacag
     1561 tcatttttga tgaaatatag gactatggga taagcgcgca agcagagaag cctatgggag
     1621 aaggatctcc cggcgctgaa acatcccctc gccaatcatg cgcatgaaca tatcggcgat
     1681 ctgctgaggg gtatactgac cctcggcgcg gtaccactgc gagagccagt tgcacatccc
     1741 aagtaaaccg aaggtcgcaa ttttgtggtc gatatccgcg tgaaacacgc ccgccgcctg
     1801 gccctcctcg atgatctgct cgaaaaagcg ctcgtaagct ttcttacgca gcaaaatctc
     1861 tttagaaaac gggccggtca gatgtggatg ctcgcggtag aagacggtca tagcaggcga
     1921 acggtcggca gccacaacaa catggtgttc caccgcgcgc cgcagcttct cgccgggcgg
     1981 caaatcctcc tcgacgatct tgcgcagcgg ctcttcgctg gccgtcagca ccgtcaaata
     2041 aatcagccgc aggatttcct ctttgttcct gacgtggtaa tacaacgcgc ccttcgtcac
     2101 ccccagcaca gatgcaatat catccaggct ggtcgcgcca aagccatcgc gcgcgaacaa
     2161 ctcggctgcc gccgccacaa tcgccttctg cgtcagccgc acgcgcttga ttttaccatt
     2221 ggccggtttg ctgccgtttt tcctggaggt ctgcgtcatt ctagattcct gggtatactc
     2281 gatagtggat ttctactgat tgttagaata ataagtggta ttcgaagctt tgtcaatatg
     2341 cagcgaatat gcaacccgta ggagccggtt taccgtgctc ctacggggct tccaaccccc
     2401 tcttgacttt cccaatatta ttgtttacaa ttctaccagt cgttcaaatt tgtgatg
//
LOCUS       DASZAK010001315         1760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_495254, whole genome shotgun sequence.
ACCESSION   DASZAK010001315 DASZAK010000000
VERSION     DASZAK010001315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495254"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1760
                     /locus_tag="VGT44_23010"
     CDS             <1..>1760
                     /locus_tag="VGT44_23010"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEV2583749.1"
                     /translation="QQFATETLGKGVVLCKDTPNFIANRIGVYGMLSTIRRAIDEGYT
                     VSEVDAILGPSMGRPRSAIFRTADLSGLDTSAHVANNLYENAPNDEQRETFRVPEALS
                     EMIQRGWLGEKSGQGFYKRIKNPDGESTILELNLNTLEYQPQQKMRYASLGAARGIDD
                     PTRRMLAVLDGDDRASQLARETTADSLIYAAVRAPEIADSIVAIDEAMRWGFNFDLGS
                     FEVWDVLLQHPQTFAKVFAGRELPELVQRVQSVGKGTFYTGTPGNRQYFDFHTNSYKP
                     VPVLKGAISLAAAKTANRVLRENGSASLINIGDGVACLEFHSKMNTIDEGIIEMLRYV
                     AEEGQQHFRAVVIGSEAADFSAGANVMLVLMGAKAGEWQQIENAINALQQAHQLLKYS
                     PIPIVAAPAGRVLGGGCEIVMHSSHARAFAETYIGLVEVGVGLVPAGGGCKELLIRLG
                     ANLESQLARKTGGPFTPSRRAFETIAFATTSTSAVEARDLRFLRKSDAITINRDLLLR
                     DAKADALRLAEAKEAGQWQPAQPTLLLLPGAGARMVLEQQIDNLLLTGNKVSEHDAVI
                     GRHLAKIVTGGNCSPIIPVA"
BASE COUNT          353 a          532 c          573 g          302 t
ORIGIN      
        1 cagcaatttg ccaccgagac gctgggcaag ggcgtcgtgt tgtgcaaaga tacgcccaac
       61 ttcattgcca accgcatcgg cgtgtacggt atgttgtcca ccatccggcg cgccattgac
      121 gaaggctata ccgtcagcga agtggatgct atcctggggc cgagcatggg ccgtcccagg
      181 tcggccatct tccgcacggc tgacctctcc ggcctggaca cctcggcaca cgtggccaat
      241 aatctctatg agaacgcccc caacgacgag cagcgcgaga ccttccgggt cccggaggcg
      301 ttgagtgaga tgatccagcg cggctggctg ggcgagaaaa gcggccaggg cttctacaag
      361 cgcatcaaga accctgatgg cgaatcgaca atcctggaac tgaacctcaa cacgctcgaa
      421 taccagccgc agcagaagat gcgttatgcc tcgctgggcg cggcgcgcgg catcgatgat
      481 cccacgcggc ggatgctggc cgtgctggat ggcgacgacc gcgcgtcgca gctggcacgc
      541 gagacgaccg ccgattcgct gatctacgcg gcagtgcgcg cgccggagat cgccgacagc
      601 attgtggcca tcgacgaggc catgcgctgg ggcttcaatt tcgatctggg cagcttcgag
      661 gtgtgggatg tgcttttgca gcacccgcag acgtttgcaa aggtcttcgc cgggcgcgaa
      721 ctgcccgaac tggtgcagcg tgtacagagc gttggcaagg gtacatttta tactggtacg
      781 ccaggcaacc ggcagtactt cgatttccat accaacagct acaagccggt gcctgtgctg
      841 aaaggcgcta tctcgttggc cgcggcgaag acggcgaaca gggtcttgcg cgaaaacggc
      901 tcagcctcgc tgatcaacat tggcgacggc gtcgcctgcc tggagttcca ctccaagatg
      961 aacaccatcg atgaaggcat catcgaaatg ctgcgctacg tggccgaaga aggtcagcag
     1021 catttccgcg ctgtggtgat cggaagcgag gcggcggact tttcggcggg ggcgaacgtg
     1081 atgctggtgc tgatgggtgc gaaggcgggc gagtggcagc agatcgagaa cgctatcaac
     1141 gccttgcagc aggcgcatca actgctcaag tacagtccta tccccatcgt ggccgcgcct
     1201 gccgggcgag tattgggagg aggatgtgaa atagtgatgc acagcagcca cgcgcgcgcc
     1261 tttgccgaaa cgtatatcgg cctggtagaa gtcggcgtcg gtttggtgcc ggcgggcggc
     1321 ggctgcaaag agctgctgat acgcctgggc gcgaacctgg agtcgcagct ggccaggaag
     1381 accggcggcc cattcacacc ctcgcgccgc gccttcgaga ccatcgcctt cgccaccact
     1441 tcgaccagtg cggtggaggc gcgagacctg cgtttcctgc gcaaaagcga cgccattact
     1501 atcaaccgcg acctgctgct gcgcgacgcg aaggccgacg ctctgcgcct ggccgaggcg
     1561 aaggaggccg gtcaatggca accggcgcag cccacgctgc tgctcctgcc tggcgcgggc
     1621 gcgcgcatgg tgctggagca gcagatcgac aacctgttgc tgacgggcaa caaggtcagt
     1681 gagcatgacg ccgtgatcgg tcggcacctg gcaaagatag tgacgggcgg caactgctcc
     1741 cccatcatac ccgtggcaga
//
LOCUS       DASZAK010001316         5857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_389880, whole genome shotgun sequence.
ACCESSION   DASZAK010001316 DASZAK010000000
VERSION     DASZAK010001316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389880"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VGT44_23015"
     CDS             complement(<1..271)
                     /locus_tag="VGT44_23015"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2583750.1"
                     /translation="MENKASDTAGVPEISSPRFAALYNWLMGRPLVRRVFDPLRHEIL
                     GQAQGAVLEVGAGGGQNFPFYDPARVVRVEAVEPDEAMLAAARCRL"
     gene            complement(354..905)
                     /locus_tag="VGT44_23020"
     CDS             complement(354..905)
                     /locus_tag="VGT44_23020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583751.1"
                     /translation="MIENQPFLVTAFVNRLSKSSPKARDHPTTDQTTNHHREIGPLGT
                     AVRLLVGLLLVGLIVYGQLASSGHLTPVTWVLGLLGFPAPVLAWHAWRIRRHPAPFSD
                     TNPLSFVLSLVLPLVFYLTGWLVPPLWFSSDATLIFVGASLLLAALRGSAGCEFLALS
                     NWLLRRHDQLACAVFTPIDSLER"
     gene            complement(927..1247)
                     /locus_tag="VGT44_23025"
     CDS             complement(927..1247)
                     /locus_tag="VGT44_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583752.1"
                     /translation="MFPYEAMHDERESGSPGFVSGEELLFNSSAGQKLSQDALGQAPT
                     VGQRLTLALVSLGMLMGMTTLLTILATARSAPAWEVFPFLFILTLFTAAVISINVAFS
                     RRVW"
     gene            complement(1313..1861)
                     /locus_tag="VGT44_23030"
     CDS             complement(1313..1861)
                     /locus_tag="VGT44_23030"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV2583753.1"
                     /translation="MYSEILILAMLRQGPRHGYEIKKDIDRALGGMVVLNNKTLYLAL
                     KHFEERGAVTRQVIPQEGKPKRHLYKLTERGIELVHAYLRDFPAALASNDAEFFTRVA
                     FFDLLEGPEREAVLTTRLAFLEGCLSYLQQMQKLAEGEACAHPADLSMSPAQRVLAFH
                     TRRIHDEYAWIASWLEEMQASR"
     gene            2161..2694
                     /locus_tag="VGT44_23035"
     CDS             2161..2694
                     /locus_tag="VGT44_23035"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEV2583754.1"
                     /translation="MNRPNQRIRKPSKRVLLPHWMALILTPVVFLLGHVAVPQELSLL
                     SARHGWADARPGWLNLLGLLPIGAGFSGLVWCLRLHFVGARGSFETEGTQNYLVVRGP
                     YRFTRNPMYLCGMLIWLGWIILYGSVAVLAGAVVFWGSVTGLVVPWEEHKLEARFGET
                     YLRYKNSIPRWVGKRSR"
     gene            2986..3939
                     /locus_tag="VGT44_23040"
                     /pseudo
     CDS             2986..3939
                     /locus_tag="VGT44_23040"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            4127..>5857
                     /locus_tag="VGT44_23045"
     CDS             4127..>5857
                     /locus_tag="VGT44_23045"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV2583755.1"
                     /translation="MASEIKHAIEENRAVPQTQTQSTAENRTVLALEGMTCASCAMRI
                     EKGLKKVPGVKDASVNFATEQATVTYDPAQTRLEQMVQKVDSVGYKAIPLVMPASRPV
                     ENEPDSESRTVLDLEGMTCASCAMRIEKGLKKVPGVIDANVNLATEQAIVIYDSTQTN
                     LEQMVQKVEAVGYKATPQVTSTRQPLQGTTTIEVTETPGLHVLSITQEDELSRRRQAE
                     ITRKRNLLILGIVLTLPVVILSMFFLNRFPGENYLLLVLTTPVWAIVGWEFHRGAIKT
                     LRHGSANMDTLISLGSTAAYVMSVVATFFPQVVGPITFYDTTALIVTLIFLGKYLEAR
                     AKGQTSEAIRKLIGLQARTAHVVRAGEEIELPIEQVQVGDQLIVRPGEKIPVDGAVLS
                     GISSVDESMITGESIPVEKVEGEPLIGATINQRGLLRMRATKVGADTVLANIIRMVEQ
                     AQGSKAPIQRLADTISGIFVPIVLVIAAITFIGWAIIGNASVSNTAAIMGASNPWIIA
                     IVAAVAVLVVACPCALGLATPTAIMVGTGKGAEQGILIKGGESLERIHAVRAVLLDKT
                     GTITKGKPELT"
BASE COUNT         1279 a         1643 c         1708 g         1227 t
ORIGIN      
        1 ccaggcggca tctggccgcg gccagcatgg cctcatccgg ctcgactgcc tccactcgca
       61 cgacgcgggc tggatcgtag aaagggaagt tctggcctcc accggcaccc acttccagca
      121 cggctccttg tgcctggccg aggatttcgt gccgcagggg atcgaacacc cgcctgacca
      181 ggggccgccc catcagccag ttgtacaacg ccgcaaaacg tggggaggag atctccggca
      241 ccccggcagt atcgctcgct ttgttctcca tcgcctcgct cctctcgctt gtctcaaagg
      301 gacggtgatt ggctgacgcc ttgcagactc ctgcaaaggg aggggcaact ccttcaccgt
      361 tccagggagt cgatgggtgt gaagacggcg caggcgagct ggtcgtgacg gcgcaggagc
      421 cagttggaga gtgccaggaa ctcgcatcca gcggagcctc ggagtgcagc gagcagcagg
      481 gaagcgccaa cgaagatgag cgtggcgtcg ctgctgaacc acaggggcgg caccaaccat
      541 cccgtgagat agaagaccag gggcagcaca aggctgagca cgaagctcag cgggttggtg
      601 tcggagaagg gtgccgggtg gcggcggatc cgccaggcgt gccaggccag cacgggggcg
      661 gggaagccga ggaggccgag tacccaggtg acgggcgtca ggtgccccga cgatgccagt
      721 tggccgtaca cgatgaggcc gacgagcagc agtcccacca gcaggcgcac cgccgtgccg
      781 aggggtccga tctcgcggtg atggttggtc gtctggtcgg tggtggggtg gtctctcgcc
      841 ttcgggctgg atttgcttag gcggttgaca aaagcggtca ccagaaatgg ctggttttcg
      901 atcatcggtt cctccttcgt gatggttcac cacactctgc ggctaaaggc gacattaata
      961 ctgatcaccg ctgccgtgaa cagagtgagg atgaagagga aagggaacac ctcccaggcg
     1021 ggagcgctcc ttgcagtcgc caggatggtc agcagcgtgg tcatacccat cagcatgccc
     1081 agcgaaacca gggccagtgt caaacgctga ccgacggtgg gagcctggcc gagggcatcc
     1141 tgggagagct tttgcccagc gctgctgtta aaaagtaatt cctcaccact cacaaaccct
     1201 ggagagccgc tctccctctc gtcatgcatt gcttcgtatg gaaacatact gttgctcctt
     1261 cttaggcgtg tacatgccgc caagcggacc gggtactcgc gacgggacgc cttcaccggg
     1321 aggcttgcat ctcttcaagc caactggcaa tccaggcata ttcgtcgtgg atgcgtcggg
     1381 tatgaaaggc caggacccgc tgcgcagggg acatcgagag gtccgcagga tgtgcacatg
     1441 cctcgccctc cgccagcttt tgcatctgtt gcagatacga gaggcatccc tccagaaaag
     1501 ccaggcgagt ggtcaagacc gcctcgcgct ctggcccctc cagcaagtcg aagaaggcga
     1561 cccgcgtaaa aaactcggcg tcattgctcg cgagcgcggc gggaaaatct cgcaggtagg
     1621 cgtgcaccaa ttcgatcccg cgctcggtca gcttgtagag atggcgcttg ggcttgcctt
     1681 cctgggggat gacctgccgg gtgacggccc ccctctcttc aaagtgcttg agagccagat
     1741 agagcgtctt gttgttcagg acaaccatcc cacccagcgc ccggtcgata tcctttttga
     1801 tctcgtagcc gtgccgggga ccctgccgca gcatggcaag aattaagatc tcgctataca
     1861 tatgatactg ctctatatga tactgttatc tatcaaataa gaataccaga gaggggcgag
     1921 gatgtcaagg gtatctgttg aaaagacgtg aaggcagaag cgagtcagtg gcgagtctgc
     1981 ctcttgtaat cctggaatat cccatgaatt cgtgatcgag ggagggcctc ggaaacctgg
     2041 tcaaatgaga ctggactctg gtgggcagca tctgctggaa gaacaatcac caggactgaa
     2101 acagatgaac cttgttcttc cccgtgaaaa gctttacaat gggatgaggc gaggaaagcg
     2161 atgaacagac ccaaccaacg catcaggaaa ccatccaaaa gagtccttct tccccactgg
     2221 atggcactca tcctgacgcc ggtggtgttt ttgctcgggc acgttgccgt gccacaggaa
     2281 ctctcgttgc tctcagcccg tcacggatgg gcagacgcgc ggccagggtg gctcaatctg
     2341 ctcggactcc tccccatcgg ggcaggcttc tcaggtctcg tttggtgtct tcgtctgcat
     2401 tttgtcggcg ctcgtggctc ctttgagacg gaggggaccc aaaactatct ggtggtgcgt
     2461 ggcccctacc ggttcacgcg caatccgatg tacctctgcg gcatgctcat ctggctgggc
     2521 tggatcatct tgtatggcag cgtcgcagtc ctagccggcg cagtggtctt ctgggggagt
     2581 gtgaccgggc tggtggtgcc gtgggaggag cacaaactgg aggcgcgatt tggcgagacg
     2641 tatctgcgct acaaaaacag tatcccgcgg tgggtgggca aaaggagccg ctgatgtcct
     2701 ggcttcccac gggagtccag agacctcgcc tccaagaagg ttcatcaacc cctcccattt
     2761 tcttccagca gatgctgccc accagagtcc agtctcattt gaccaggttt cgggcctcgt
     2821 ctctacttct tgccacaggg aaacggagtt tgtcgaactg tgctgcaaag caggcttatc
     2881 ggcacagttt cccttcccac gtggtatatt atcggcagag ctaaccgaat tatctggcca
     2941 agaactgcgc cgataatgca cctgtgccga taacaaggag acgtcatgtg gaatgagatt
     3001 gctgcctggc aggagacgct gacccatcgt cctgatctgt ctgccaaaac ggttgctttg
     3061 tatacccagg atgctcgtcg ctttgccact tggctccaga gtgaacatcc tggtctggga
     3121 gtaaccgaga tcacacccat tgatgccaaa acgtatcgtg atcacctgct cgccaaacgc
     3181 ttagctccca cgaccatcaa tcgtgcgctc atcagccgga tgctcttctt tgatacggtt
     3241 gatggcacga atccctttcg ccacctgacg atggttgatg tgattgaacc tgcgcccgtc
     3301 gccctctcaa agaccgaatg gaacgcggtc cggcggtgtg cagagcaact cgttcgtcgc
     3361 gatcacggac tggcattagc tctcgctact ctcttccgct atgcaggtcc ccgcgtctcc
     3421 gaagtggccg cgctccagat tcccgatgtc caggtctcag cacggcgcgg tttgctcatc
     3481 attcgacgtg gcaagggact caaacatcgc gaggtcccgc tcgtcccgga agcccgcgaa
     3541 ccgctcgatg cctatttcga ccaccgcgaa tatctggctg aacgctagcg agaacgagca
     3601 gcgatccggg gagaagcagt tgcggcctgg agtttctggc ccgatgggca tctttttctc
     3661 ggtcagcgtg gccccttagc tgaacgcggc attcgtgaga ttattgccaa gcttggtcag
     3721 gccgccaaac tctcttcccc tctcggccct cacgatctgc gccatacgtt tgccaaagca
     3781 ctgcttgacc ttgccgctta tggcctggat cggcctccga tgccacttcc agcgattcag
     3841 caattgctcg gccatgctga cattgcgacg actaccatct acacacgggt ctctgctgat
     3901 gatcttgtcc gaatgatggg cacatccgaa gcatactgag acaaaactgt gccgcactta
     3961 tctactttgc ggctacgagg ccccttcctt aacacagggc ctaccacgca cgattaagaa
     4021 tttctcactc caagccttga cataatgctc aatgatacgc tatactatct atacccccac
     4081 caggggggta tgggtaaata tatacgcaaa gacaagagta atcatcatgg cttcagaaat
     4141 taagcatgcg atagaagaaa atagagcagt acctcagacc cagactcagt cgacggcaga
     4201 gaacagaact gtgctcgcat tggaaggaat gacctgcgcc tcgtgtgcca tgcgcatcga
     4261 gaagggactc aaaaaggtgc ctggagtgaa agacgcaagc gtcaacttcg ccaccgagca
     4321 ggccaccgtt acttatgatc ctgcgcaaac aaggctggag cagatggtgc agaaagtaga
     4381 ctcagtgggg tacaaggcaa taccgctcgt catgcctgca tcgaggcctg tggagaatga
     4441 acccgatagt gagtctcgta cagtccttga cctggaagga atgacctgcg cttcctgtgc
     4501 tatgcgcata gagaagggac tgaaaaaggt gccgggtgtg attgatgcga atgtcaatct
     4561 cgcgacggaa caggccatcg tcatctacga ctcaacgcag acaaacctgg agcagatggt
     4621 gcagaaagta gaagcggttg gatacaaggc aacgccccaa gttacatcta cacggcaacc
     4681 attgcaaggg acgacaacca tcgaagtcac cgaaacgccg ggcttgcatg ttttgagtat
     4741 cacacaagaa gatgagctca gcaggaggag acaggcagag atcactcgta agcgcaacct
     4801 gttgatcctg ggtatcgtgc tcacactgcc tgtcgtgatc ctcagcatgt ttttcctgaa
     4861 tcgcttccct ggtgagaact atctcttact cgttcttacc acccctgtgt gggcaatcgt
     4921 gggttgggag tttcaccggg gagctatcaa gacgctgcgc catggtagcg cgaatatgga
     4981 tacgctgatc tcactcggct cgacagcagc ctatgtcatg agcgtcgtgg cgacgttttt
     5041 tccgcaagtt gttggtccta tcacctttta tgacaccacc gctttgatcg ttacactgat
     5101 cttcttgggc aagtatctcg aggcccgcgc taaggggcag accagcgagg cgatcaggaa
     5161 attgatcggg ctgcaggccc gtaccgccca tgtcgtgcgt gcgggagaag aaatcgagct
     5221 gcccatcgag caggtgcagg tcggcgatca actcatcgtg cgtcctggcg agaagattcc
     5281 cgtggatggg gcagttctct ctggcatctc atcggtcgac gaatcgatga ttaccgggga
     5341 aagcattccg gtggaaaagg tcgaggggga gccactgatt ggagcgacga ttaaccagcg
     5401 tggactattg cgcatgcgcg caacgaaagt cggggctgat accgtccttg caaacatcat
     5461 ccgcatggtc gagcaggccc agggatcaaa ggctccgatc cagcgtttgg ctgataccat
     5521 ttcgggcatc ttcgtcccca ttgtgctggt gatcgcggca attaccttca ttggctgggc
     5581 gattattggg aatgcgagtg tgtcaaatac tgctgctata atgggcgcta gcaatccctg
     5641 gatcattgcg attgtggcgg cagttgcggt gctagtggtt gcctgcccct gtgctttggg
     5701 gttagccacg ccaactgcta tcatggtagg aacgggcaaa ggcgctgagc agggtatact
     5761 gatcaaaggt ggtgagagcc tggaacgcat ccatgcggtg cgtgcagtcc tgcttgacaa
     5821 gacaggcacc attaccaagg gcaagccgga actgacc
//
LOCUS       DASZAK010001317         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_138667, whole genome shotgun sequence.
ACCESSION   DASZAK010001317 DASZAK010000000
VERSION     DASZAK010001317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138667"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VGT44_23050"
     CDS             <1..773
                     /locus_tag="VGT44_23050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2583756.1"
                     /translation="PPSGILPAGAPGIRVGPGMAGPPGTALAGQATGLIRAAQDHLAA
                     GRIEQAYAAIQQAYQYDPNNALVHKIYGQVFARRIPPNADLAIQAYNRSVMANPNDAE
                     THKLVGDVFYFLRKQPAQAIPPYTQSIRLNTNDFETHQRLAKCYEETNQLDAAAREYQ
                     EAIRLSPPTIKRTELNYSLGQLTYRMRQWPLAERAFVQVLTANPADHATRFLLSQVYE
                     QEGKLADALRECNFVVQATPTNQAAQIMLQRLNGQAAH"
     gene            850..>2189
                     /locus_tag="VGT44_23055"
     CDS             850..>2189
                     /locus_tag="VGT44_23055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583757.1"
                     /translation="MNTHRFIPSAHHGMGRFALSLCGGLLLALSLLLPPTASAAAPSA
                     PSLSSANVLNLAGVSVVRLSMTYISSKGIKITCTALGTLIASWPASSNNDANNWVVTD
                     STALSLTGGTGKCAPVGDTLATIQILANNLYTSATNPNTTQATLDTLQCGKTTCQDQT
                     SESLSSTAIGGGVLFSFHTDGLNMQPFLDTGIGQPSLSTSVGIELANQSKVGAWPPKP
                     QLTDALQALTFLTPVDAISNAPSLTPGATPSSAPAPGNNVTSFEPGMPLVDAQGAFIG
                     MKVSNSTTLFTVSNLKTLEMAFSTLQSQKSLEANNILKVQWNNGIQQFDQNSFLAAKN
                     DFDTILDHTKVNNPQFQAANTFSQQAAAKLPHPTGSPTTAGSPTTGQGQGGSGLGLFG
                     STLLMIGLGAGLILLVLLLILVSLVFGRRRALRKKNQEEMDRFKEEAAAARRNAP"
BASE COUNT          484 a          711 c          573 g          421 t
ORIGIN      
        1 ggcccccatc gggcattctt ccggcaggcg cgccaggcat ccgcgtgggc ccaggcatgg
       61 caggaccgcc tggcacagct ttggcaggtc aggcaaccgg tctgatacgc gctgcgcagg
      121 atcaccttgc ggcgggtcgc atcgagcaag catacgcggc catacagcag gcttaccaat
      181 atgacccaaa taacgcgctt gtgcataaga tatatggcca ggtcttcgcg cgccgcatac
      241 cacccaatgc cgacctcgcc attcaagcct ataaccgctc agtaatggcc aatcccaacg
      301 acgccgaaac gcataagctg gtgggcgatg tcttctattt cctgcgtaag cagcctgcac
      361 aggccattcc gccatatacc cagtccattc gtctgaacac caacgatttt gaaacgcatc
      421 agcgcctggc aaagtgttat gaagagacta accagttaga cgcggctgcg cgcgagtacc
      481 aggaagccat ccggctgtca cctccaacga tcaagcgcac agaattgaac tactcgcttg
      541 gacaactgac ctaccggatg cggcagtggc ccctggcaga gcgagctttc gtgcaggtgc
      601 tgacagccaa tccggccgat catgcaactc gtttcctgct gagccaggtc tacgaacagg
      661 aagggaagct ggccgatgcc ttgcgcgaat gtaactttgt cgtacaggct actccgacaa
      721 accaggcggc acagatcatg ttacagcgcc tgaatggtca ggcggcgcat tgacatagtt
      781 gtgaaagcgg agagatgata ccaaatccat agttatagga gacccgatac aaaggggtta
      841 gagcgaaaga tgaatacgca tcgatttata ccttcggcgc accatgggat gggaagattc
      901 gcgctgtctc tgtgcggtgg gctgttgctg gcgctatcgt tgctgctccc acctaccgcc
      961 tctgccgccg ccccgtctgc tccatcgctc tcatcggcca atgtcctgaa cctggcgggc
     1021 gtctccgtcg tgcgcctctc gatgacctat atctcttcta aagggataaa gatcacctgc
     1081 accgccctgg gaacgctcat cgccagctgg cccgcctcct caaataacga tgcgaataac
     1141 tgggtcgtga ccgacagcac cgcgctctcc ctgaccggag gcaccggcaa atgcgctccc
     1201 gtcggcgaca ccctggccac cattcaaatt ctcgccaaca acctgtacac cagcgctacc
     1261 aacccgaata ccacccaggc cacgctcgac acccttcaat gcggcaaaac cacctgtcag
     1321 gatcaaacca gcgagtccct gtcctctacc gccatcggcg gcggcgtcct gttctcgttc
     1381 cacaccgacg gtctgaatat gcagcccttc ctggataccg gtatcggaca gcccagcctg
     1441 tccacgagcg tcggcatcga gctggcaaac cagagcaagg tcggtgcgtg gcccccaaag
     1501 ccacagctca ccgacgctct ccaggcgctg acctttctga cgccggtcga tgctatctct
     1561 aacgctccat cactcacgcc aggtgccaca ccgagcagcg cgcctgcgcc gggcaacaac
     1621 gtcaccagtt tcgagccggg catgcccctc gtcgatgcgc agggggcctt catcggtatg
     1681 aaggtctcca actcgaccac gctcttcacg gtctccaatc tcaagacgct ggaaatggcc
     1741 ttttcgacgc tgcaatccca gaagtcgctt gaagccaata atatcttgaa ggtgcaatgg
     1801 aataatggta tccagcagtt cgatcagaat agttttctcg cggcaaagaa tgactttgac
     1861 acgatcctcg accacacgaa ggtcaacaat ccgcaatttc aggccgccaa cactttttcg
     1921 cagcaggcag ccgccaagct ccctcatccc accggttcgc ccacaacggc cggttcgccc
     1981 actaccgggc aaggccaggg tggctccggg ctgggtctct tcggttcgac gctcctgatg
     2041 atcggcctgg gtgcagggct gatcttgctt gtgctgctcc tcatcctggt cagcctggtc
     2101 tttgggcgac ggcgcgcgct gcgtaaaaaa aatcaggaag agatggatcg cttcaaggaa
     2161 gaggccgccg cggcacgccg caatgcccc
//
LOCUS       DASZAK010001318         1673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_352807, whole genome shotgun sequence.
ACCESSION   DASZAK010001318 DASZAK010000000
VERSION     DASZAK010001318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352807"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1256)
                     /locus_tag="VGT44_23060"
     CDS             complement(<1..1256)
                     /locus_tag="VGT44_23060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583758.1"
                     /translation="MDFQIVLSVLSKMRQFRPRDHWTRQQLESYQADALRRLRDYTYA
                     HSPFYQQFHKGLYDAPLQELPVLTKAMMMEHFDELVTDRAIRLEEAKTHMRTLTGDEL
                     FLGRYRVNATSGSSGHPGIFLFNRAEWITVLASFARAREWGGIKLDLIHRVKTATVAS
                     TTAFHMSSRVNATAHSWWMPEIRLAASEPLETIVARLGAWQPEMLIAYASMARILADE
                     QIAGRLQIRPRAVFTSSEVLTPQTRRRIVQAWGERLFNQYAATESGSLAAECDHHRGM
                     HLMEDLVIFEVVDQDNRPVPPGVYGDKLLITVLFNHTQPLIRYELSDSVRLATNPCPS
                     GHPFALIDGIQGRVEDVLSFPGAAGGVVNVQPLVFSRIMDTIPVSGWQVIQEADGLRV
                     LLSGVRGEFGDEMLADTLRQALAEQRV"
     gene            complement(1303..>1673)
                     /locus_tag="VGT44_23065"
     CDS             complement(1303..>1673)
                     /locus_tag="VGT44_23065"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEV2583759.1"
                     /translation="ARALVNDPALILADEPTANLDSKIGHEIMRLLQTIAKEQGRSVV
                     IVSHDQRIQDIADRVLWLEDGQFKEMATMATDPVCGMKIEREKAATTEWEGQSYYFCS
                     RGCRSEFLEGPHQFLNTIPQ"
BASE COUNT          327 a          501 c          478 g          367 t
ORIGIN      
        1 accctttgct ccgccagtgc ctgccgcaag gtatctgcga gcatttcgtc tccaaactcg
       61 ccacgcacac cgctcagcag cacacgcaag ccatcggcct cttgtatcac ctgccagcca
      121 ctcaccggta ttgtatccat gatgcggctg aagaccagcg gttgcacatt caccactcca
      181 ccagcggcgc ccggaaagga cagcacatcc tccacacgcc cttgaatacc atcgatcagc
      241 gcaaaggggt gcccggaagg gcagggattc gtggccaaac gcacactgtc gctcaattcg
      301 tagcggatga gcggctgggt atggttgaag agtacagtga tgaggagttt gtcaccgtag
      361 actcccggcg gcaccgggcg gttgtcctgg tctacgacct cgaagatcac gagatcttcc
      421 atgagatgca tgccccggtg gtggtcgcac tcagcggcaa ggctgccgct ttcggtggcg
      481 gcgtattggt tgaagagccg ctcaccccag gcctgtacaa tgcgccgccg tgtttgcggt
      541 gtcagcacct cggaactggt gaacacggca cgtggcctga tctggaggcg tcccgcaatc
      601 tgctcgtcgg ccaggatacg agccatggag gcataggcaa tcagcatctc tggttgccag
      661 gcacccaggc gcgcgacgat ggtttcgagc ggttcgctcg ccgccaggcg aatttccggc
      721 atccaccagc tatgggccgt tgcgttgacc cggctcgaca tatgaaaggc ggtggtcgag
      781 gcgacggttg ccgttttgac gcggtgtatc aggtctagct tgattccgcc ccattcgcgc
      841 gcgcgggcaa atgaggccag cactgtgatc cactcggcgc ggttgaagag gaagatgccg
      901 gggtgaccac tgctgcctga cgtggcgttg acccggtacc gcccaaggaa aagctcatcg
      961 ccggtcaggg tcctcatgtg cgtctttgcc tcttccaggc gaatggcgcg gtctgtcacc
     1021 agttcgtcga agtgctccat catcatcgct tttgtaagca ccggaagctc ctgtaatggg
     1081 gcgtcgtata gccccttgtg aaactgctgg tagaatggcg aatgtgcgta ggtgtagtcg
     1141 cgcagcctgc gcaatgcgtc cgcctggtat gactccaatt gttggcgtgt ccaatgatct
     1201 ctcgggcgaa actgccgcat cttcgatagc acactcaaaa cgatttgaaa atccatgata
     1261 tcctcctaat tgtccaggca tttcagtgtg gatttcgctc ttctattgag gaatggtatt
     1321 caagaactgg tgaggcccct ctaaaaactc gctgcggcag cctcgcgaac agaagtagta
     1381 ggactgcccc tcccattcgg tcgtggccgc cttctcgcgc tcgatcttca tgccacagac
     1441 cggatcggtt gccatggtcg ccatctcctt gaactgtcca tcctccaacc atagcacgcg
     1501 gtcggcgata tcctggatgc gttgatcatg ggaaacgatg accacacttc ttccctgttc
     1561 ttttgcgatc gtttgcagca gccgcataat ctcatgcccg atcttcgagt cgagattcgc
     1621 cgtcggctcg tcagccagga tcagggccgg gtcattcacc agggcgcgag cta
//
LOCUS       DASZAK010001319         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_602609, whole genome shotgun sequence.
ACCESSION   DASZAK010001319 DASZAK010000000
VERSION     DASZAK010001319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602609"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /gene="proS"
                     /locus_tag="VGT44_23070"
     CDS             <1..1406
                     /gene="proS"
                     /locus_tag="VGT44_23070"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV2583760.1"
                     /translation="GATHEEVVTLLAAEFARSYRDLPQLIYQVQTKFRDEPRPRRGLL
                     RQREFLMKDLYSFDADEDGLDASYRKVAQAYHTIFKRCGLRYVAVEADSGAIGGKQSQ
                     EFLAITEAGEDDAMLCDTCGYAANREKAEFVRSEQELESEAAIEEVFTPGRESIFAIM
                     EFLHVPVTKTMKSVCYSAGGRIIMAMVRGDLEINEVKLANALYHTGINAAGLRMAMPE
                     ELAQAGIVAGYTSPVGKDERVYIVADPSLRLGNNFVAGANKANYHFINVNYPRDYRVD
                     VWEDIASAPDGAACARCSGGKLDAVRGCEIGHIFKPGSYYSDLFGATYLDAAGVARPL
                     LMGSYGIGIGRIMATAVEQNHDEKGIIWPSSIAPYHVALVGLDVDREESRQAAEQLYA
                     DLCASGVEVLYDDRAESPGVKFNDADLLGLPLRVVVSKRSLKNGGVELMRRSSRESRV
                     VPLNAAIEVITHDSTRP"
     gene            complement(1381..>2324)
                     /locus_tag="VGT44_23075"
     CDS             complement(1381..>2324)
                     /locus_tag="VGT44_23075"
                     /inference="COORDINATES: protein motif:HMM:NF013950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="G1 family glutamic endopeptidase"
                     /protein_id="HEV2583761.1"
                     /translation="SMTLSAYVFGQSVTNLAVKFTIKAAGQSPVTICQSVVSDVDTYS
                     CNWDFTLNGALFHNGPVTLGFMIAGNRQSGGTFGPAVNPDGVRAGRITYEQTQASTNY
                     AGYAATNLSGTTDYQKVTGSWTVPSVSCSPGELSASAFWVGMTSRDETSKSLLAQLGT
                     DSDCQNGTPIYYLWWEMFPAPSMTLQLPLQPGDVATASVIFQQGTFQLTIDVPNEGDH
                     FSINQPGSISSTSLAECIVEAPFLIDNPATGQGHIIQLTNFGQISLSCQINNNEPVAN
                     GPQDYVYQMQTDFGTNKATTSGLDQSGTTFTVVWNHG"
BASE COUNT          479 a          640 c          722 g          483 t
ORIGIN      
        1 tcggcgcaac acacgaagag gtagtgacgc tgttggccgc cgagttcgcg cgcagctatc
       61 gcgacctacc ccagctcatc taccaggtac agaccaaatt tcgagatgag ccgcgtccgc
      121 gcaggggctt gctgcgccag cgcgagttct tgatgaaaga cctgtacagc ttcgatgccg
      181 acgaggacgg cctggacgcc agctatcgca aagtggcgca ggcgtatcac accatcttca
      241 aacgctgcgg cctgcgctac gtcgcggttg aggcggatag cggcgcgata ggcggcaagc
      301 agtcgcagga atttcttgcc atcacggagg caggggaaga cgacgcgatg ctctgcgata
      361 cctgcggcta cgcggccaac cgcgaaaagg ccgagtttgt gcgcagcgaa caggagttgg
      421 agagcgaagc cgcgattgaa gaggtcttta ctcccggtcg cgaatcgatc ttcgctatca
      481 tggaatttct gcatgtgccg gtcacgaaaa ccatgaaatc cgtgtgctac tcggctggcg
      541 gcaggatcat catggcgatg gtgcgcggcg acctggagat caacgaggtc aaactggcga
      601 acgcgctcta tcacacgggc atcaacgcgg caggcttgcg catggccatg ccggaggaac
      661 tcgcccaggc gggcatcgtg gcgggttaca cctcgcctgt aggcaaggat gagcgggtgt
      721 atatcgtggc cgacccctcg ctccgcctgg gcaacaactt tgtcgcgggg gcgaacaagg
      781 cgaattatca tttcataaac gtcaactatc cgcgcgatta tcgcgtcgat gtttgggaag
      841 atatcgcctc agcgcctgat ggcgctgctt gcgcgcgctg cagcggcggt aagctggatg
      901 cagtgcgcgg ctgcgaaata ggccacatct tcaagccggg tagctattac agcgaccttt
      961 tcggtgcaac ctacctggac gcggctgggg tggcgcgccc gctgctgatg ggcagctacg
     1021 gtatcggcat cgggcgcatc atggcgacgg cggtcgagca aaaccacgat gagaagggca
     1081 tcatctggcc atccagcatc gcgccctacc acgtcgcgct ggtggggctg gacgtagacc
     1141 gggaggagtc acgccaggcc gctgagcagc tctacgccga cctgtgcgcc tccggcgtcg
     1201 aagtcctcta cgatgaccgc gccgagtccc ccggtgtcaa attcaacgat gccgacctgc
     1261 tcggcctgcc actgcgcgtg gtggtgagca aacgctcgct gaagaatggc ggcgttgagt
     1321 tgatgcggcg ctcgagtagg gaaagccgtg tcgtgccgct taacgcggcg attgaggtaa
     1381 tcacccatga ttccacacga ccgtaaacgt tgtgccagat tgatccagcc cggatgtagt
     1441 tgctttgttg gttccaaaat cggtctgcat ctggtagaca taatcttgcg ggccgttggc
     1501 taccggctcg ttattgttga tctggcagga gagactgatc tggccgaaat tggtgagttg
     1561 tatgatgtgt ccttgcccgg tggcaggatt atctatcagg aatggcgcct ctacaataca
     1621 ctccgccagg ctggttgagg agatgcttcc aggttggttt atggaaaaat ggtcgccctc
     1681 attgggaacg tcgatggtga gctggaatgt tccttgctgg aagatcacgc tggccgttgc
     1741 gacatcgccg ggctgcaagg gcagttggag ggtcatagac ggggccggga acatttccca
     1801 ccataaataa tagatgggag tgccgttctg gcaatcgcta tcggtgccga gttgcgccag
     1861 cagactttta ctggtttcgt ccctgctggt catacccacc caaaatgctg aagcggaaag
     1921 ctcgccgggc gagcaactta ccgatggaac cgtccagctt ccggtcactt tttgatagtc
     1981 ggtcgttccg ctcaaattcg tagcggcgta ccccgcgtag ttggtggagg cttgagtttg
     2041 ttcatacgta attcgcccag cgcgaacacc gtccgggttg acagccggtc caaaggtgcc
     2101 tccgctttgc cggttgccag cgatcatgaa gcccagggta acaggcccgt tgtggaacaa
     2161 ggcgccattc agcgtgaaat cccagttgca ggaataggta tccacatcgc ttactacact
     2221 ctggcagatc gtgactggcg actgcccagc tgccttgatg gtaaatttca ctgccaggtt
     2281 ggtaacgctt tgcccaaaca cataggcgga taaggtcatc gatg
//
LOCUS       DASZAK010001320         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_246437, whole genome shotgun sequence.
ACCESSION   DASZAK010001320 DASZAK010000000
VERSION     DASZAK010001320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246437"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VGT44_23080"
     CDS             <1..243
                     /locus_tag="VGT44_23080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583762.1"
                     /translation="LAGANGLYLLFLLLGGGILPLSHLPVGLAALAQLLPAAALTQAL
                     QASLSNGTTFPTGALLTLVTWAVLILLVSIRMFRWE"
     gene            complement(275..793)
                     /locus_tag="VGT44_23085"
     CDS             complement(275..793)
                     /locus_tag="VGT44_23085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583763.1"
                     /translation="MKRWMLALLVVGAVVLVGGVFVFRFAQARLAPPPVFSHLARAPN
                     LVYKFKLCLEAFTSDSINADPAYKTNYTFRDPFRSAEDTIIPTLISWNSSKKTASFQV
                     VIIVGQIQTFRETITQVSALDGIFVMLPDTQDLLQRANEPQFVVSAINASGSAAVLDY
                     TCSQEWVWGRVK"
     gene            complement(880..1389)
                     /locus_tag="VGT44_23090"
     CDS             complement(880..1389)
                     /locus_tag="VGT44_23090"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEV2583764.1"
                     /translation="MERRRLAKSQVFLLACSLAGAGVAVYLTSVHYQLAPLACSESGF
                     VNCARVLSSTYSVVPGTTIPISIPGLAWSLVMVALVLIELRHPTRWLQLAQFVWSLVG
                     MLTVLYLVYVEIVLLHNLCAWCTAMHVLIFLIFLVALARLAPSQPEALANEAADVESA
                     ETIHVADRR"
     gene            complement(1404..>2300)
                     /locus_tag="VGT44_23095"
     CDS             complement(1404..>2300)
                     /locus_tag="VGT44_23095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEV2583765.1"
                     /translation="SFSIQRGKTLGLVGESGSGKTTIGRTIARLYKPTAGQMLFDELD
                     LATLNGESLRQARQRIQMVFQDPYASLNPRFTIGSLVAEPMHIYHVGSSKEIRDRTVE
                     LLSVVGLRPEYIDRYPHEFSGGQRQRIAVARALAINPEFVIADEPVSALDVSIRAQVL
                     NLLQRLQQQFNLTYLFVSHDLSVVRHVADRIAVMYLGKIVELADRDELYNAPKHPYTK
                     ALLSAIPIPDPQIEKHRQRIILSGDLPSPIKIPSGCRFHTRCPMAQEICREIEPQFEA
                     KEGHEHFAACHFSEKVTPALEA"
BASE COUNT          525 a          634 c          673 g          468 t
ORIGIN      
        1 ctggcgggcg ccaatgggct ttacctgttg tttttgttgc ttggcggagg cattctgccc
       61 ttgagccatc tcccggtggg attggccgcg ctcgcgcaac tgctgcccgc cgccgcgctc
      121 acccaggcgc tgcaagccag cctgtcgaac gggacaacct tccccactgg cgcgttgctg
      181 accctggtca cctgggcagt cctcatcctg cttgtatcga tccgcatgtt ccgctgggag
      241 tgagtcacgc atgaatggag aatatgggcc ggggttattt cacccggccc cacacccatt
      301 cttgcgagca agtataatcc aacactgcag cggacccgga ggcattgatt gcgctcacca
      361 caaattgcgg ctcattcgca cgttggagga gatcctgtgt gtcgggtagc atgacaaaga
      421 tgccatccag cgccgaaacc tgcgtgatag tctcacggaa tgtctgtatc tggccaacga
      481 tgatcaccac ctggaagctc gccgtctttt tggatgagtt ccagcttatc agcgtgggaa
      541 taattgtatc ctcggcgctg cgaaaggggt ctcgaaaggt atagttagtt ttataggcag
      601 ggtctgcatt gatggagtcg gatgtaaatg cttcgaggca caacttaaac ttatatacca
      661 ggttgggcgc gcgcgccagg tgcgagaaga caggcggagg cgcaagcctt gcctgcgcaa
      721 accggaagac aaacacacca cccaccaata caactgcgcc cacgacaagt aaagcgagca
      781 tccagcgttt catcgtgaca cctcctgcta acgaggacga gagtcatgcc tcttcaatag
      841 aggctacacg aatacagaac aatctacgct gaatggcttc tagcgacggt cagcgacgtg
      901 gatggtctcc gccgattcaa catcagctgc ttcgtttgcc aatgcttcgg gttgagatgg
      961 cgcgaggcgc gcgagggcaa ccaggaatat caaaaaaatc aacacgtgca tagcagtaca
     1021 ccaggcgcac aaattgtgaa gcagtacaat ctcgacgtag accaggtaca gaacggtcag
     1081 cattccaaca agcgaccata caaattgcgc cagctgcagc cagcgcgtgg ggtggcgcaa
     1141 ctctatgaga accagcgcca ccataaccag gctccacgcc agccccggaa tgctgatggg
     1201 gatggtcgtc cctggaacca ccgaatacgt gctggagagc acgcgcgcgc aattgacaaa
     1261 gccgctctcc gaacaggcca ggggagccag ctgataatgc accgacgtga gatagacggc
     1321 gacgccggcc ccggccaggg agcaagctaa taaaaaaacc tggcttttcg cgagacgtct
     1381 tctctccatg ctgtccttat tttctaagcc tctaaagcgg gcgtgacctt ctccgagaaa
     1441 tggcaggccg caaaatgctc gtgtccttct ttggcttcaa attgcggctc gatttcgcgg
     1501 caaatttcct gggccattgg gcagcgagta tggaaacgac agcccgaggg aattttgatg
     1561 ggactgggaa gatcaccaga aagtataatg cgctggcggt gtttctcgat ctgcggatca
     1621 gggatgggta tcgccgaaag cagcgccttt gtatagggat gtttgggcgc gttgtacagc
     1681 tcgtcgcggt cagccagttc gacgatcttc cccaggtaca tgaccgcgat gcggtcggcg
     1741 acgtggcgta ccacgctgag gtcgtgcgag acgaacaggt aggtcagatt gaactgctgc
     1801 tgcaatcgct gcaagaggtt gagcacctgc gcgcgtattg agacatccag ggcggagaca
     1861 ggctcgtcgg cgatcacgaa ttcggggttg atggcgaggg cgcgcgcgac ggcgatacgc
     1921 tggcgctggc cgccgctgaa ctcgtgtggg taacggtcaa tatattctgg acgcaggcca
     1981 accacgctca gcaactcgac ggttctatcg cgaatctcct tagaagaacc tacatggtag
     2041 atatgcatcg gctcggcgac gagcgaccca attgtaaaac gcgggtttag cgaagcgtaa
     2101 gggtcctgga agaccatctg aatacgttga cgcgcctgcc gtagtgactc gccattcaga
     2161 gtagcgaggt ctagttcatc gaacagcatc tgcccggcgg tcggcttgta gaggcgtgcg
     2221 atcgtgcggc cgatcgtagt cttccccgag ccgctctcac caacaaggcc gagcgttttg
     2281 ccgcgctgga tggaaaagct
//
LOCUS       DASZAK010001321         2197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_246453, whole genome shotgun sequence.
ACCESSION   DASZAK010001321 DASZAK010000000
VERSION     DASZAK010001321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246453"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..531
                     /locus_tag="VGT44_23100"
     CDS             130..531
                     /locus_tag="VGT44_23100"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV2583766.1"
                     /translation="MSINEHQSTIEEVTGAPNAPDVCGDTARAEMIMERAMCLLGDKW
                     TLMIVYNLRHGKRRFGELLEVLGNISPKTLSQRLKQLEEIRLVERRAYAEIPPRVEYL
                     LTDRGLMLTNILKAIQEFGENCLSDIPNLSL"
     gene            complement(523..1494)
                     /locus_tag="VGT44_23105"
     CDS             complement(523..1494)
                     /locus_tag="VGT44_23105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583767.1"
                     /translation="MLLTLPLILFIVMTELSIGAFAILFLLDWRNQVKRSFLVVYTFI
                     YLFLTGLTYLFQQNYSTAALLNSFSQLDKGWTGYLALPLLLFLLLMVVYTVFLLADKK
                     AGVDGKVEGRTIISRRRWLRLVAGSLTALAGLMTLFVLGMIYRPLASSSLAGVFIVSG
                     FFAAALALGGVMTAMWLGHWYLVTPALSEKPLQFAATLVLLGVLAEAVFAFTGTINAP
                     AQSIVSATPTVTITATATAAPGATATPGAQVKPAGVPTITPLSVGAIGWLRILVAFVM
                     PLVLLVFILKLIHDRSFQSATGMLYLVVVLTLAGEAMARGLFLMGLQ"
     gene            complement(1590..2111)
                     /locus_tag="VGT44_23110"
     CDS             complement(1590..2111)
                     /locus_tag="VGT44_23110"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV2583768.1"
                     /translation="MRTSLVQILETQRLKLLPFTLELKKATLAERARLTEMLAVKIPD
                     DWPGADLLEALPFFIEGMEKDAAGLVWDGIIIQKAEQIAIGGIGFHGGPDEAGMVEIG
                     YNIIPAYEGQGYATEMARCVIDWAFHTPGIQVITAECLDDNIGSIRVLEKVGMRRLAP
                     KGTLLKWELQLHP"
BASE COUNT          529 a          634 c          603 g          431 t
ORIGIN      
        1 ggcacaaggc ccccgccgct ccaccctgcc tctccctgtc cctacatacg ttctgcgatt
       61 agagcgcgaa aggaatgatg ttcgagctgg tttcagccat aggggaacaa agtatggtat
      121 acttaacaca tgagtataaa cgaacatcaa tccacaatcg aagaggtaac aggcgctccc
      181 aatgcccccg atgtctgtgg tgacactgcg cgggcagaga tgattatgga gcgtgccatg
      241 tgcctgttgg gggataagtg gacgttgatg attgtctata acctgcggca tggcaaacgg
      301 cgttttggag agctgcttga agtactcggc aacatcagcc ctaagacgct atcgcagcgc
      361 ctcaagcagt tggaagagat caggctggta gagcggcggg cctacgcgga gataccgccg
      421 cgcgtggaat acctgctgac cgatcgcggc cttatgctca cgaacatcct caaggccatt
      481 caggagtttg gcgagaactg cctctcggac attcccaacc tttcactgta accccatcaa
      541 gaacagcccg cgcgccatcg cctcgcccgc cagggtcagc accaccacca ggtagagcat
      601 ccccgttgcc gattggaatg atcgatcgtg gattagctta aggatgaaga cgagcaggac
      661 gaggggcatg acaaaggcca ccaggatgcg cagccagccg atagcgccca cgctgagcgg
      721 cgtgatggtc ggcacaccgg cgggcttgac ctgcgcgccg ggagtggcgg tcgcgccagg
      781 ggcagccgtt gcggtggcag tgatcgtcac cgtcggggta gctgaaacga tcgattgcgc
      841 cggggcgttg atcgtgccgg tgaaggcgaa gaccgcctcg gccaggacgc cgagcagcac
      901 cagcgtggcc gcgaactgca agggcttttc cgacagcgcc ggtgtgacca ggtaccagtg
      961 tcccagccac atcgcagtca tgaccccgcc gagcgcgagc gcagccgcga aaaagccact
     1021 cacgataaag actcccgcca ggctggacga ggccaatggg cgatagatca tgcccagtac
     1081 gaacagcgtc atcaaccccg caagagcggt caggctgccg gctaccagcc tcagccagcg
     1141 tctccgcgat atgatcgtgc gtccctcgac cttgccatcc acgcccgcct tcttatcggc
     1201 cagcaggaat accgtataga ccaccatcag cagcaggaag agcagcagcg gcagagccag
     1261 gtagcccgtc cagcccttgt caagctggct aaagctgttg agcagcgccg ccgtcgaata
     1321 attctgctgg aataaatagg tgagacccgt cagaaaaaga tagataaacg tgtagacgac
     1381 gagaaaactg cgcttgacct ggttgcgcca atccagcagg aacagtatgg caaacgcccc
     1441 gatggacagc tccgtcatca cgataaacag gatcaacggc aacgtgagta acatagtata
     1501 tattcaatcc ttcatagcat catcaggtgc gaaacgggtt ttggccgttc cactatcgag
     1561 acagattata cagtaagctg gcaaataagt taaggatgta gctgaagctc ccacttgagc
     1621 agcgtgcctt tcggtgcgag gcgacgcatc ccgacttttt cgaggactct gatggagccg
     1681 atattgtcat ccaggcattc agcagttatc acctgaatac caggcgtatg aaaagcccag
     1741 tcgatgacgc aacgcgccat ctcggtggca tatccttgcc cttcatatgc gggaatgata
     1801 ttgtagccaa tctccaccat tcctgcctcg tccggtccgc catggaaacc gatgccgccg
     1861 atagcgatct gctcggcttt ctgtataata atgccatccc agacaaggcc cgccgcatcc
     1921 ttctccatac cttcaataaa aaaagggagc gcttccagca aatcggctcc tggccagtca
     1981 tcaggaatct ttaccgcaag catctccgtg agcctggctc tctcagccag cgttgccttc
     2041 ttgagttcca gcgtgaaagg gagcagtttg agccgttgcg tttccagtat ttgcacaaga
     2101 gatgtcctca cttccgtagt tccttctagc tattgaaagg cagcgtccat ctaagaaaac
     2161 ttttcctggg gcagtatatc atgtctggga cgaagta
//
LOCUS       DASZAK010001322         8053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_106181, whole genome shotgun sequence.
ACCESSION   DASZAK010001322 DASZAK010000000
VERSION     DASZAK010001322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8053
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106181"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1352
                     /locus_tag="VGT44_23115"
     CDS             126..1352
                     /locus_tag="VGT44_23115"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2583769.1"
                     /translation="MTTSRSYKAGLLLLCIASVLAGCNSANTGPSGQTPVTYSIVYGC
                     SDDALTTKSSLPPSHITAYIASFSGSLYAFDTGNGVLRWCLHIQAHGSFSAYAKVAGL
                     AGPPPIPVGLSTPVLAGGILFTDSEDGYVYAFKADTGSLLWQTQSPTGIAYDIGQGPA
                     VVNSTVYASSSNGLFALNAWNGVLRWQSLSGVMLHGTPVIAGNTVYVGADNGKMYAVD
                     ASTGALRWQFQTSTEIQTSPVVVNGILHLVNHDGSAYALDAATGKALWRSRTGYLITP
                     WITSGDGRLYITASDSFIYTLDAATGKLLWRYQTSVGIYAPPSLNNGVLYVASSGIYS
                     LRASDGQLLWHNILDTHLYGLTTPTILRGIIYTTSQGNGSLAPAAMHELRAQDGQLLF
                     SFPIGYNGSNSAPAIA"
     gene            1449..3146
                     /locus_tag="VGT44_23120"
     CDS             1449..3146
                     /locus_tag="VGT44_23120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583770.1"
                     /translation="MQTQRPDSDTIKTLSSPPATAPRGRRRWLTGLGALLVVALLIAS
                     SVVVFSLVGNSHGNQGHGTTGQWKAVQQGYLFLSIKAAPSNPAVLYACATTSAAVSNQ
                     GAPGTVTVLRSADGGDTWQNIGANLLQNSACDLAINPANSDDIYVFSGGNSSQSPAVL
                     HHSTDGGDTWQAIEPRVYISPTRTTQRIFLQQLTFAGNSLLAINWIYTPVPIGIVSPP
                     NLPFTLPRLVRSTDGGQNWTIIDSQLSTQNIAAQSFAVDPSQPDTIYELAGHPYLPVE
                     RPPSGGVPAYGVYQQLYKTTSGGNSWTLVLDKALYGSQVQIAANNPSIIYVGGILGPL
                     PEGAQPQLGAAGAPTGSSTGATTLPQIIGGFHLQVSRDGGATWQAVTPLPDQAAILNW
                     FVSADGHVFSSPTTSSGAGVSGTAIIVGTAVAGTAVVVPPCCKTGGTPQRGSVYPPVP
                     GTAVGNTSGSSSGLADVSPPPGKSPILNYDPASNSWSNVIDPPGIGEMLAVTTGPHGE
                     TVLWFMAVGTSGTQYTLYRDIVELESQRGQSRHSSNFIWRGQSCHAERSEASLVGYA"
     gene            complement(3185..4270)
                     /locus_tag="VGT44_23125"
     CDS             complement(3185..4270)
                     /locus_tag="VGT44_23125"
                     /inference="COORDINATES: protein motif:HMM:NF027641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HEV2583771.1"
                     /translation="MKRTRILLVSYLKSVVLLALVLLTLAAVVVGGYDGIGARASGDR
                     TMPIGRPIINRAPVFPVVINRPALAFYYSWYNPQTWCSCTMSDLPTIKYRSSDESTIE
                     RQVNAAANAGITGFISSWWGQGDQTDSNLAKVLAYSATLERTTHLLFASTIYFESDAP
                     ALQGEGAIVRQLRYLVAHYANSRYFFHWQGKPVIFFWDEFGHGRTAALWAHIRSQVDP
                     HHTMIWSAEGTDLNALSVFDGLHLFSAAYWGIQHNNIAAVDKGFRDEIDAYNKAHHTQ
                     KIWAAGVLPGYNDTRVPGRQGTYVVPRNNGATYRESWNGAVASNPDWITITSYNEWFE
                     GSMLEPSVTYGNLYIDLTGQLTSKWRG"
     gene            complement(4417..7731)
                     /locus_tag="VGT44_23130"
     CDS             complement(4417..7731)
                     /locus_tag="VGT44_23130"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEV2583772.1"
                     /translation="MVKAPLSHRGECSRRRFVLFALPLVCMLVMGSLVSFRLLSIGTS
                     VQASGLVRIAGHVPELENKSTLLGSTDPNTPVQIVIGLRPRNAQALKSQADALAQVRQ
                     AQTRRYLTPDQVATAFAPAAATQSALIAYMQGQGFSLTNSYRDRLVVGFKGTIGIAEK
                     AFGVQINNYRAPGGRVYYAPSNEPGMPANLAPQVQSIVGLDDTIQYTHPPVQGKSIAT
                     RTVAQPNAASCLPAQPGPIYTYYLPSQIQSAYNLVGLYNQGYQGQGQTVALLELDDYT
                     ASDISAYTACYGGASVPISRVLVNGGTGMPPGSNANEVELDIDLVLSAAPKLAGLKVY
                     EAAATTAGYLAVWTQIVNDAVPIVGSSWGDCETNTTSAVYQQENTLFQVAELQGQSLF
                     AAAGDKGTNDCASIPAKFAVDDPASQPYVTGVGGTSLTIGPNNAYGSEAVWNDSFGAG
                     GGGVSSLWTMPTYQSGDANTISATYSSRTPCGAAAGTYCREVPDVSINADPKVGYPIY
                     CTVAASCGPGNTWVAVGGTSAGPPLWAAMTAILNQKLLQNGFFNVGFLNGYLYAIDQG
                     QNGTHYANDFHDVTVGTNDTVNSTHTYPATSDYDMTSGLGSYNAQNLVSDVASIVKAT
                     VAPRTNEANTTWYFAEGSVGGSFTEYLTILNPDPVNIATIQVDYLFQNQPAVLKLHQV
                     LPSTRFTINVNSDLNIASTAPQQAISMIVQSIGIGSTAPVPVVAERPMYFNYRGVKSG
                     TDVLGATNATNTTFYFAEGDNRQSGNAHYSTFITVLNPSNIQTATVTATFYTGACGLS
                     SQPACPTSAITVPPLYRGTILPPVHSQFSAMVTSTSGVVVERPMYFTDVIPTAGGATS
                     GSASAVGATALGPSTGSDWLFAEGYTGPNFQEYLVLADFTSTNTQADVKLEYTNGTVQ
                     TVPVTVPALSQVYFDVNHAYTNPVPGCNCTPTQSVSAEVIASTASIVAERLMYFHYGP
                     QLLSGATDVVGQAGPASKANFSFAEGYTNTNFSEYLTIQNPLNSVNTVSITLFADNTI
                     VEEMLQLQPHSRTTVFINSFIAPMAAAYPTNPVYLGYNVSMSLESSSPIVAERPLYFN
                     YSGDTGGTDVIGYSGG"
BASE COUNT         1780 a         2452 c         2154 g         1667 t
ORIGIN      
        1 ctgtttgtct acaagtattt cctaattctc aacacaatag ggaacacttt gcactccaat
       61 ttcgtcctag ttatgacgga caatcgacgc cagaatattt acatcaaatg aaagaatcca
      121 cgcgcatgac aacatcacgc tcctacaagg caggtctgct gttgctctgc atcgcaagcg
      181 tactggccgg ctgcaatagt gccaataccg gaccatcagg acaaacgccg gtgacgtaca
      241 gcattgttta cggctgtagt gatgacgcac tgactacgaa atcatctctg cctccatccc
      301 acattaccgc ctacatcgcc tctttcagcg gctcgctgta tgccttcgac accggcaacg
      361 gcgtcttgcg ctggtgtctg cacattcaag cgcatggatc gttttctgca tacgctaagg
      421 tcgcaggttt agcaggcccg ccgccgatcc ccgtcggact gagtaccccc gtactcgccg
      481 gtggcatact cttcaccgat tccgaggacg gctatgtgta cgccttcaaa gcagatacgg
      541 gaagcctcct ctggcaaacg caatcgccca ccgggatagc gtatgacatc gggcaagggc
      601 ctgctgttgt gaacagcaca gtctatgcca gcagctccaa cggcctgttt gccttgaatg
      661 cctggaatgg tgtcctgcgc tggcaatctc tctcaggcgt aatgctccac ggtacccctg
      721 taatcgccgg aaataccgtg tatgtcggcg ctgacaatgg gaaaatgtac gctgtagatg
      781 cctcgaccgg cgctctgcgc tggcaattcc agacctccac tgagatccag acatcgcccg
      841 tcgtcgtgaa tggcatcctc catctcgtca accacgatgg cagcgcctac gccctcgatg
      901 ccgccaccgg caaagcgctc tggcgcagcc gcaccggcta cctgattact ccctggataa
      961 cctctggcga tggcaggctc tatatcaccg catccgacag ctttatctat actctggatg
     1021 ccgccaccgg gaaactcctc tggcgctacc agacctcagt aggcatctat gcgcctccct
     1081 cgctcaacaa tggcgtgcta tacgtcgcat ccagcggcat ctacagcctg cgcgcgagcg
     1141 atggccaact cctctggcac aacatcctcg acacccatct ttacggcctg accaccccga
     1201 ccatcctgcg cggcatcatc tacaccacct cgcagggcaa cggtagctta gcgccagccg
     1261 ccatgcacga actgcgcgcg caagacggcc agctactctt ctccttcccc atcggctaca
     1321 atggcagcaa ctccgcgcca gccatcgcct gacccaacgg gaactttcac cccctcaata
     1381 tcgtcataac atgaggaatg gatcattgca ggtgcgaccg aagagaccgg ttgaaaagga
     1441 gacctaccat gcagactcag cgacctgatt ctgacacaat aaaaacgtta tcatccccac
     1501 cagcgaccgc gccacgcggg cgacgtcgct ggcttaccgg gctaggagca ttgctggtgg
     1561 tcgcgctatt gatcgcctcc agcgtggttg tcttctcgct ggtgggcaat tcacacggca
     1621 atcaaggcca cgggactacc gggcagtgga aagccgtaca gcagggctac ctgttcctgt
     1681 cgatcaaagc cgcccccagc aatcctgccg tattatatgc ctgcgccaca acttccgccg
     1741 ccgtctctaa ccagggcgcg ccgggtacgg tgacggtatt gcgcagcgcc gatggcggcg
     1801 atacgtggca aaacatcggc gcgaacctct tgcagaacag cgcctgtgac ctcgctatca
     1861 accccgccaa cagcgacgat atctacgttt tcagcggggg caattcgtcc cagtcgccag
     1921 ccgtgctgca tcacagcacc gacggcggcg atacctggca ggccatagag ccgcgcgttt
     1981 acatatctcc aacccgaact acgcagcgca tcttcttgca acagcttacc ttcgcgggca
     2041 atagcctgct ggcgatcaac tggatttaca cacccgttcc tattggaatc gtgtcgcccc
     2101 caaacctgcc gttcacgctg ccgcgcctgg tgagaagtac cgacggcggg caaaactgga
     2161 caatcatcga cagccagtta agcacacaga acatcgccgc gcagagcttt gccgtcgatc
     2221 cctcgcagcc cgacaccatt tatgaattag ccggacatcc ctacctgccg gtcgaacgcc
     2281 ctccatcggg cggcgtcccc gcctatggcg tctaccagca gctctacaaa acgaccagcg
     2341 gcggcaatag ctggacactg gtcctggaca aggcgctcta tggttcgcag gtacagatcg
     2401 ccgccaacaa cccgtcgata atctatgtgg gcggcatcct ggggcccctc ccggagggcg
     2461 cgcagccgca actgggcgcg gctggcgcgc caaccggcag ctcaaccggc gcgacgacac
     2521 taccgcaaat catcggcggc ttccatctcc aggtcagccg tgacggtgga gcaacctggc
     2581 aagccgtgac gccgctgcca gatcaggcag ccatcctgaa ctggttcgtc agcgccgatg
     2641 gccacgtctt ctcctctccg accacctctt ccggtgcggg tgtatcggga actgcaatca
     2701 ttgtgggaac agccgtcgcg ggaacagcgg tagtggttcc tccatgttgt aagacaggag
     2761 gaacaccgca gcgcggcagc gtctatccgc cggttcccgg cacagcggta ggtaacactt
     2821 cgggcagctc atccggcctt gccgacgtct cgcccccgcc gggcaaaagt cccatcctga
     2881 actacgaccc ggccagcaac agttggagca atgtcatcga ccccccaggc atcggcgaga
     2941 tgctggcagt gacgactggc cctcatggcg agaccgtgct atggttcatg gccgtcggaa
     3001 catcgggaac gcagtacacg ctctaccgcg atatcgtcga gttagagagc cagcgaggtc
     3061 aaagtcgtca tagctccaat ttcatatgga gaggccaaag ctgtcatgct gagcgcagcg
     3121 aagcatctct cgtcggctat gcatagatgc ttcgctgcgc tcagcatgac agctttggcc
     3181 tctgttatcc ccgccatttc gaggtcagtt gcccggttaa atcaatatac aggttgccat
     3241 aggtgacact cggctccaac atcgaccctt caaaccattc attgtagctg gtaatcgtaa
     3301 tccaatcggg attactggca accgcgccat tccagctctc gcgataggtt gcgccgttat
     3361 tacgtgggac aacatacgtt ccctggcggc ctggcactct ggtatcgtta tagccgggca
     3421 ggacgccagc cgcccagatc ttctgtgtat gatgcgcctt attgtaggcg tcgatctcgt
     3481 cgcgaaaccc cttatctacg gcagcgatat tgttatgctg gataccccag taggccgcgc
     3541 tgaacaggtg caggccatca aagaccgaca gcgcgttcag atcggtgcct tccgccgacc
     3601 agatcatcgt gtgatgcgga tcaacctggc tgcggatatg ggcccacagc gcagccgtgc
     3661 gcccatgtcc gaactcgtcc cagaagaaaa tgaccggttt gccctgccag tgaaaaaaat
     3721 accggctgtt ggcatagtgg gcaaccaggt agcgcagttg ccgcacaatg gccccctcgc
     3781 cttgcagggc cggagcatca ctttcaaaat agatcgttga agcaaatagc agatgcgttg
     3841 tccgttccag ggttgccgag tacgccagca ccttcgccag gttgctatcg gtttggtcgc
     3901 cctgccccca ccacgatgaa atgaagccgg taataccggc gttcgcagcc gcgttcacct
     3961 gccgttcaat ggttgattcg tcacttgacc tgtatttgat ggtcggcagg tcagacatgg
     4021 tacacgaaca ccatgtttgg ggattgtacc aggaatagta aaaggccagc gcgggccggt
     4081 tgataacgac cggaaacacc ggggcgcggt tgatgatggg acggccaatc ggcatcgtgc
     4141 gatcaccgct cgcccgcgcg cctatcccat catagccgcc aaccaccacc gccgcgagcg
     4201 tcaacagaac cagcgccagc agaacgacac tttttaggta agaaacaagc agtattcgcg
     4261 tacgtttcat agccgattac ctcctctggg atagatcatc ctgatttaca gtataccaat
     4321 acaggtaatc gtgcatcaga taaaatataa tataggaaaa tggtcacgag ggcgaccctc
     4381 tactgcggtc gctcttgcat tctgcggtcg cccttgtcat cctcccgaat agccaatcac
     4441 atcggtgcct ccggtgtcac cgctgtaatt gaagtagagg ggacgttccg cgacgatagg
     4501 gctactggat tcaagactca tcgagacgtt gtaacccagg tagaccggat tggtgggata
     4561 ggccgccgcc atcggcgcga taaagctgtt gatgaacacc gtcgtgcggc tgtgcggctg
     4621 caactgcaac atctcctcca cgatcgtgtt atccgcgaag agcgtgatgc tcacagtatt
     4681 gaccgaattc agcgggttct gaatagtcag gtactcggag aagttcgtgt tggtatagcc
     4741 ttcagcgaag gagaagttcg ccttactggc tgggccggcc tgcccaacca cgtccgtcgc
     4801 gccagagagc aactgcggcc cgtaatggaa gtacatcagg cgttccgcca cgatagatgc
     4861 tgtgctggca atcacctcgg ctgataccga ctgcgtgggt gtacaattac agccgggaac
     4921 agggttggtg taagcatggt tcacatcgaa gtagacctgg ctgagcgcgg gcaccgtgac
     4981 cggcaccgtc tgcaccgtgc cattggtata ttcgagcttc acgtccgctt gcgtattcgt
     5041 gctggtgaag tcggccagca ccaggtactc ctggaagttc ggccccgtgt agccttccgc
     5101 gaagagccag tcgctgccgg tgctgggacc gagcgcagtc gcgccaaccg cggatgccga
     5161 gccgctcgtc gcgccgccag ccgtcgggat gacatccgta aagtacatgg gtcgctcaac
     5221 cacaacgccg ctggtcgatg tcaccattgc cgaaaattgc gaatgcaccg gcggcaaaat
     5281 cgttccgcga tacagcggag gcaccgtaat cgccgacgtc ggacaggccg gctggcttga
     5341 cagaccacac gcccccgtat agaacgtcgc tgtgacagta gcggtctgga tgttgctcgg
     5401 attcagtacg gtaataaacg tgctatagtg tgcgttgccg ctctgacgat tgtcgccctc
     5461 ggcaaaatag aacgtcgtat tggttgcgtt cgtcgcgccc agcacatccg tgccgctctt
     5521 cacgccgcga tagttgaagt acatcgggcg ttccgctaca acgggtaccg gggcggtcga
     5581 gccgatgcct atcgattgca cgatcatcga gatggcttgc tgcggggccg tagaagcgat
     5641 attcagatcg ctattgacat tgatcgtaaa gcgcgtactc ggcaagacct ggtgcagttt
     5701 cagcaccgcg ggctggttct ggaagagata atccacctgg atcgtggcga tattgactgg
     5761 gtcaggattg aggatggtca ggtactcggt gaagcttccc ccgacgctgc cttcggcaaa
     5821 gtaccaggtt gtattcgcct cgtttgtgcg cggcgcaaca gtcgccttca cgatcgacgc
     5881 cacgtcgctc accaggttct gcgcgttata actccccagc ccagatgtca tatcgtaatc
     5941 tgacgtggcc ggataagtgt gcgtactatt cacggtgtca ttagtgccga ccgtcacatc
     6001 atggaaatcg ttggcatagt gcgttccatt ttgtccctgg tcaatggcat acagatagcc
     6061 attcaagaac cccacgttga aaaatccatt ttggagaagc ttctgattta atatggcggt
     6121 catcgccgcc cacaatgggg ggccagccga cgtgccgcca accgcgaccc aggtgtttcc
     6181 aggaccgcac gacgctgcta ccgtgcaata tatcgggtag ccaacctttg gatcggcgtt
     6241 gatcgagaca tcaggtactt cgcgacaata cgtgccagca gccgcgccgc atggagtgcg
     6301 ggagctgtag gtcgcgctga tcgtgttcgc atcgccgctt tgataggtag gcattgtcca
     6361 gaggctgctc acgcctccgc cgccagcccc aaagctatca ttccagaccg cctctgagcc
     6421 gtaggcatta tttggcccaa tcgtcagcga cgtgccgcca acacccgtca catacggctg
     6481 cgaggcagga tcatcaaccg caaatttggc aggaatgctg gcgcaatcgt tagttccctt
     6541 atcccccgcc gctgcgaata gagactgccc ctgcagttca gcaacctgga acagcgtatt
     6601 ttcttgctgg tagaccgccg aggtagtgtt ggtctcgcaa tcaccccaac tgctgccgac
     6661 gatcggcaca gcgtcattga cgatctgcgt ccaaacagcg aggtacccgg cggtcgtagc
     6721 ggctgcttca taaactttca aacccgccag tttgggagcc gcgctcaaaa ccagatcgat
     6781 atccagttca acttcattag cattgctgcc ggggggcata ccagtccccc cattgaccag
     6841 cacccggctg atcggcacag atgcgccgcc ataacaggca gtataggcgc tgatatcact
     6901 agcggtataa tcatcgagtt cgagcaaggc aacagtctga ccctggccct gatatccctg
     6961 attatagaga ccaaccaggt tataggcgga ctgtatctgt gagggaagat agtacgtata
     7021 tatcggaccg ggctgcgccg gcaaacaact cgccgcgttg ggctgagcaa ccgtccgggt
     7081 agcgatgctc ttcccctgta ccggcgggtg ggtatattgt atcgtatcat ctagcccaac
     7141 gatgctctga acctggggcg cgagattggc cggcatcccc ggctcgttcg atggggcgta
     7201 gtagactctc ccgcccggcg cgcgataatt attgatctgg acgccgaaag ctttttcagc
     7261 tatcccaatc gtccctttga accctacgac cagccggtcc ctatacgaat tggtcaggct
     7321 gaagccctgc ccctgcatat aggcaatcaa ggcggactgc gtggcagcag ccggcgcgaa
     7381 agcggtcgca acttgatcgg gagtcaaata gcgccgcgtt tgtgcctgac gcacctgcgc
     7441 aagcgcgtcg gcctgagact tcaacgcctg cgcattacgc ggacgcaggc cgatcactat
     7501 ttgcacaggt gtattgggat cagtcgaacc gagcagcgta ctcttattct ccagctccgg
     7561 cacatgcccg gctatccgca ccaggccgct tgcctgaacg ctagtgccta tcgacaacag
     7621 ccgaaaggag accagcgacc ccatcaccag catacagaca agaggaagcg cgaacagcac
     7681 aaagcgacgg cgcgaacact cccccctatg actcaaaggc gcctttacca tagagttgtc
     7741 tctcccaaaa atagtttctt gtttccagat tcatcataac ttgacataaa tgaaactaga
     7801 gtgtgaaccc aaagggaagt tttcataaac actgacgtgt ggctaactgc cagataacat
     7861 cggcatatat tgatgggact tgtggttacg ccctccttac gcccgttgac gcaatcagcg
     7921 catcatgaag aaattgtcat taatatggct ctgcaagcgt gaaacaggca caaaacgatg
     7981 acaatttctt catgatcgtg aaccatcgta aagtctgcat ctggaggaat taggcggcat
     8041 ggcgaccgca agg
//
LOCUS       DASZAK010001323         1709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_352924, whole genome shotgun sequence.
ACCESSION   DASZAK010001323 DASZAK010000000
VERSION     DASZAK010001323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352924"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VGT44_23135"
     CDS             <1..510
                     /locus_tag="VGT44_23135"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEV2583773.1"
                     /translation="LADLLAQLEQLDPHSASTIDRSNPRRVMRALEVCLVSGKRFSEQ
                     RGHANPLYRSLLLGISWPREVLYQRIDARVDERMRQGMVQEVVNLLAQGVSHERLDSL
                     GLEYRYISRLLRGEFASEADMTQRLKYAIHDFTRRQLSWFRRDPRITWLDGMDVEQAA
                     TLIARHNSS"
     gene            571..822
                     /locus_tag="VGT44_23140"
     CDS             571..822
                     /locus_tag="VGT44_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583774.1"
                     /translation="MTEKEYILKLMHRAFLDIRVASYSQDNHTCFVLSDVFHNIPLQI
                     NQADKGETSYTDIVIRIQKKYEERKYKSWLDNAVADMTK"
     gene            complement(950..1429)
                     /locus_tag="VGT44_23145"
     CDS             complement(950..1429)
                     /locus_tag="VGT44_23145"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2583775.1"
                     /translation="MLNAQDLSIAFARNVTIVKWQAEGLTNEDSLRQFPNGNCLNWAL
                     GHIAVNRDEVLRALGEAPEMGDAGARYKRGSEPLKDADEGILTLEELLAWLERSQERI
                     ASALDKVDETALAREFGTGDRKRTVGQSVFFLYFHESYHVGQTEMYRQFAGRGDKII"
     gene            1506..>1709
                     /locus_tag="VGT44_23150"
     CDS             1506..>1709
                     /locus_tag="VGT44_23150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583776.1"
                     /translation="MHIIRWQASTPPQERDLRNLMQQQGLSPYSWSNGPHDTYAVHSH
                     SYEKVLYSVRGSIRFTLPDRPEGI"
BASE COUNT          406 a          523 c          415 g          365 t
ORIGIN      
        1 ctggcagacc tgctcgcgca gctggaacag ctcgacccgc acagcgcctc cactatcgac
       61 cgaagcaatc cgcgtcgcgt catgcgcgcc ttggaggtct gcctggtctc cggcaaacgc
      121 ttctccgagc agcgcggcca cgccaacccg ctctaccgct cgctgctgct cggtatttcc
      181 tggccgcgcg aagtcctcta ccagcgcatc gatgcccgcg tcgacgaacg aatgcgccag
      241 ggcatggtgc aggaagtcgt caatctgctg gctcaaggcg tcagccacga acgcctggac
      301 agcctgggac tcgaataccg ttacatcagc cgcctgctgc gaggcgaatt cgccagcgaa
      361 gctgacatga cccagcgtct gaaatacgcc atccacgact tcacccgcag gcaactcagc
      421 tggtttcgcc gcgaccctcg catcacctgg ctggacggca tggatgtgga gcaggcagca
      481 acactcatcg ctcgccacaa tagctcataa aaacaacccc aaaccccact gtttattgtg
      541 aatcttattc tgctgaggaa ggagagaatt atgacggaga aagaatatat tttgaagctt
      601 atgcaccggg catttttaga tatccgtgtt gccagttact cgcaagataa tcacacgtgt
      661 tttgtactct cagatgtatt tcacaacatc cccctgcaaa ttaatcaagc tgataaaggt
      721 gagacgagct ataccgatat cgttatccgg atacaaaaga aatatgaaga aaggaaatat
      781 aaatcctggc ttgataacgc tgttgctgac atgacaaaat agctcagcga tctactcaat
      841 aggataccct acggaatcat ccagggatgc agtgccatgt accacacgtt gccgaatggg
      901 tctttcacgc cagcaatccg tgatcggttt atcacatggc tcaccttcat caaatgatct
      961 tgtcgcccct gcccgcgaac tggcgataca tctccgtctg tccaacgtga tagctctcat
     1021 ggaagtagag gaaaaacacg ctctgcccaa ccgtccgctt tcggtcgcca gtgccaaact
     1081 cgcgcgccag ggcagtctcg tccaccttgt caagcgccga tgcgatacgc tcctgcgagc
     1141 gctcgagcca ggctagcagt tcttccagcg tcaatatgcc ttcatccgca tccttcaaag
     1201 gctcggagcc gcgtttgtag cgcgcgccag catcgcccat ctccggcgct tcacccagcg
     1261 cccggagaac ttcgtcacgg ttcaccgcga tatggcccag cgcccaattc aggcagttgc
     1321 catttggaaa ctggcgcaga ctatcttcgt tggtcaaacc ttctgcctgc cacttaacga
     1381 tcgtcacatt ccgtgcgaat gcgatggaca ggtcttgcgc gttgagcata tgaacctcct
     1441 gagattgaaa tgttgtatat tcaagtatat gacccggtga aggtaaaaga aagattcgat
     1501 tcaccatgca catcattcgc tggcaggcat ccacaccacc gcaggaacgc gacctgcgca
     1561 atctcatgca gcagcaggga ctatctccct actcgtggtc gaacgggccg catgacacct
     1621 acgccgtgca tagccacagt tacgaaaagg tgctgtattc cgtgcgcggc tctattcgct
     1681 tcaccctgcc cgaccgaccg gagggcatc
//
LOCUS       DASZAK010001324         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_425243, whole genome shotgun sequence.
ACCESSION   DASZAK010001324 DASZAK010000000
VERSION     DASZAK010001324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425243"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..365)
                     /locus_tag="VGT44_23155"
     tRNA            complement(291..365)
                     /locus_tag="VGT44_23155"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(331..333),aa:Asn,seq:gtt)
     gene            complement(435..2405)
                     /locus_tag="VGT44_23160"
     CDS             complement(435..2405)
                     /locus_tag="VGT44_23160"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV2583777.1"
                     /translation="MIPLIFLSALLRQSIFDIENHRLGILKDIYVSLNETFPVVTALL
                     IAPSNLSSSNNDLIVPWSQVDQLETEQVHVTVKQSRIAPYTPGSDELLLRRDLLDKQI
                     VDTQGFRVVKVNDLKLAQIKGTARLVGADISFSGLLRRLGILTPIQWLGKIVPVRIDE
                     RTITWNYIEPIQVMRVGTSQLAPAVAGVGGAVGTAVAGGIVPQVQLNVSHTRLADLHP
                     ADIADILEQLNAEEAGAVLDRMDTETAADILSEVESARQTEILSGLDPERASDLLERL
                     APDDAADILAEIPQEEAERLLALMPVEESQPIRDLLRYGAETAGGIMTNEVLSLSQDL
                     TVEEALAYLRQHSEHMEMVYNLYIVDDERRLVGVVSLRELVVAGPYTQLKSLMDRDVI
                     SVKVDDDQEDVARVIAKYDLLGVPVVDAENHLVGLVTVDDVIDVIHEEQAEDFSELAG
                     ASVEEFEEEEHFSLGAALSRVTWLGVNVLAGFALALLLNALLGPYLNAGAAHFQTSGL
                     TAGLRSPTAMNGLVCLLPMLLLTSGSVGTQALGVAGWQLRSANGVDFWIGIYRELRLG
                     TIGGILASIVVGLLTWLLFHSWLLGISIGLGLGLSLLIAAICGLALPALFRRLRLRGS
                     LISAPLLDPLIAAISLSIFLVAALWLIDMLHV"
     gene            3124..3196
                     /locus_tag="VGT44_23165"
     tRNA            3124..3196
                     /locus_tag="VGT44_23165"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3157..3159,aa:Met,seq:cat)
     gene            3249..3321
                     /locus_tag="VGT44_23170"
     tRNA            3249..3321
                     /locus_tag="VGT44_23170"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3282..3284,aa:Met,seq:cat)
     gene            3327..3399
                     /locus_tag="VGT44_23175"
     tRNA            3327..3399
                     /locus_tag="VGT44_23175"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3360..3362,aa:Met,seq:cat)
BASE COUNT          781 a         1087 c          991 g          745 t
ORIGIN      
        1 cctgctttcc cgatcaagca gaaaatcagc aatggcagca cgaatcgtct ttccagcccg
       61 acgcgaagcg gatgaggtgt tgctaccctt cccggtagct ttacgcgagg tttgcttgtt
      121 gcgcgtaccc ataaaaaaga agcctctgtt ctttccgcgc ctcgcaacaa gccaaccggt
      181 catagctttc catgccgaca ggtcacaaac acctgatttg cgacctcgtg acacgtagct
      241 agcgccgctt gcttgtcatc gaagcactct agaactggct tcctttggtg tggctcctcg
      301 aacaggattc gaacctgtaa cctaccggtt aacagccggc cgctctaccg ttgagctatc
      361 gaggaagatc gctcttttcg ctcttcgcga acgattgcag tatacactat gctactttgc
      421 ccgtcaatag tttttcagac atgcagcatg tcgatcagcc acagcgcggc gaccaggaag
      481 atgctcaggc tgatggcggc gatcagcgga tcgagcaggg gcgcgctaat aaggctgccg
      541 cgcaggcgca agcgccggaa tagcgccggg agcgccaggc cgcagatggc ggcgatcagc
      601 aggctcaggc cgagaccgag accgatagag atacctagca gccacgaatg gaaaagcagc
      661 caggtcagca gaccgacgac aatgctggcc aggatgcctc cgatagttcc caggcgcagt
      721 tcgcgataga tgccgatcca gaaatccacg ccgttcgccg agcgcagttg ccagccggcc
      781 acacccaacg cctgtgttcc gacgctgcca ctcgtcagca gcagcatggg tagcaggcag
      841 accagcccat tcatggcggt gggtgagcgc aggccagcgg tcagcccgct cgtctggaaa
      901 tgcgcggccc cggcgttcag gtatgggccg agcagcgcgt tgagcagcag cgccagcgcg
      961 aaaccggcca gcacgttgac gcccagccac gttacgcggc tgagcgccgc gcccagcgag
     1021 aaatgttcct cctcctcgaa ttcctccacg ctggccccgg ccagttccga gaagtcttca
     1081 gcctgctcct cgtggatcac gtcgatcaca tcatcgaccg tgaccagtcc caccaggtgg
     1141 ttttcggcat ccacgaccgg cacgccgagt aggtcatatt tggcaatcac gcgcgccaca
     1201 tcttcctggt cgtcgtccac cttgacgctg atcacatctc tgtccatcaa gcttttcaac
     1261 tgagtgtacg ggccggctac caccagctcg cgcagcgaga cgacgccgac cagccgccgt
     1321 tcatcatcca ctatgtagag gttatagacc atctccatat gctcggaatg ctgccgcagg
     1381 taggccagcg cctcttctac cgtgaggtcc tgcgaaagcg agaggacttc gttggtcatg
     1441 atgccgccgg cggtctccgc gccatagcgc agcagatcgc ggatgggttg cgactcttcg
     1501 acgggcatga gcgccaacag ccgctcggcc tcttcctggg gaatctcggc caggatgtcg
     1561 gcagcatcgt caggcgcgag ccgttccagt aggtcggagg cgcgctccgg gtcaagcccg
     1621 ctcagaattt cggtctggcg cgcggactcg acctcgctga ggatgtcggc agccgtttcg
     1681 gtgtccatgc gatccagcac agccccggcc tcttctgcgt tgagctgctc gaggatatcg
     1741 gcgatatcgg cagggtgcag atcggccagg cgcgtatggc tgacattgag ttgcacctgc
     1801 ggcacgatgc cgcccgcgac ggctgttcct accgccccac ccactccggc gacggctggg
     1861 gcgagctggc ttgtgcctac acgcatgacc tggatcggct cgatatagtt ccaggtgatg
     1921 gtgcgctcgt caatgcgtac cggcacgatt ttgcccagcc attggatggg tgtcagaatg
     1981 cccaggcgac gcagcagacc gctgaagctg atgtccgcgc cgaccagccg cgccgttccc
     2041 ttgatctgcg ccagcttcaa atcattcacc ttgacgacac gaaaaccctg cgtatcgaca
     2101 atctgcttat ccagcaggtc gcgcctcagc agcaattcgt ccgagccggg cgtgtaaggg
     2161 gcgatgcgcg actgcttgac cgtcacatgc acttgctccg tctccagctg atccacctgc
     2221 gaccagggaa cgatcagatc attattactc gaactcaaat ttgatggagc gattagaagc
     2281 gccgtgacga ccggaaaggt ttcgtttaat gagacataga tgtctttgag aatgccgagc
     2341 cggtggtttt caatatcaaa aatgctctgg cgcaggagcg cgcttaaaaa aatcaacgga
     2401 atcaccgcct ttctcattgt tcttgagaag ctagcaacat atccacagcg ctgtggatat
     2461 gtggaaaacc cctttacctc tgtaactttt gttacaagga aaaggcacag gcaagcaata
     2521 cgtatcgaat attcaggtaa cacctgaaca ttgcaacgcg cattccatgc acgatgcgct
     2581 tttggtaaac atggggacgg cttgcgctgg ttctaacacg ctaccgggaa gaaccggcaa
     2641 gcaggttggt gagctggcag gtgagaacat tcttgctcga catcacatcc gacaatgggg
     2701 gtgtcagaac tcatcgagca tggcggtgaa gaagaagttt cagcttaccc tatccgatga
     2761 ggagaagaac attcccgcga gcaggcacat gtatgacgta ttctgtatat cgtacatacg
     2821 cctactatga ctggggccag cgtccatgtc cctcacctcc ttcgcccggc tcgctgcggc
     2881 ttacgcgaac ggcagcgctc gtaataagcg cctacaacaa ctcttcgcgc gccctacaac
     2941 ggcgggtaga cgaattttta tctgtaagca gtatagcacg catgtgcaag attgagcaaa
     3001 tcttataagg ctaatgggat aaatggtaac agatttcgga aggcggcttt aagggtacgc
     3061 tctccgaaac ccttgcattt tccttgacaa cccgcaaagt ctgttgtatg ataatgaagc
     3121 cgacgcgggg tggagcagtg gttagctcgt tgggctcata acccaaaggc cggaggttcg
     3181 aatcctcccc ccgctactaa tttctacgca agtggcaatt ggctcggcaa aatacaacgc
     3241 attcttgacg cgggatagag cagtggttag ctcgtcgggc tcataacccg gaggtcgcag
     3301 gttcgaatcc tgttcccgct actcgtggcg gcgtagctca gtcggtagag caacggactc
     3361 ataagccgtg cgtcactggt tcgattccag tcgccgctac tacaactcac gctctcttgc
     3421 ccactttatc cctttctcct ggttgctctt cgccggtgca aggcgcagac gcatcggagt
     3481 tagctaaaga acagcagccc tatccccagg ccgatcagca ataccgcccc aatcagttcg
     3541 ctcgcctctt tgaacagctt cagccgcgcg cgcaaccccc tgcccagcgc tatgcccagc
     3601 gtgg
//
LOCUS       DASZAK010001325         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_138859, whole genome shotgun sequence.
ACCESSION   DASZAK010001325 DASZAK010000000
VERSION     DASZAK010001325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138859"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..463
                     /locus_tag="VGT44_23180"
     CDS             92..463
                     /locus_tag="VGT44_23180"
                     /inference="COORDINATES: protein motif:HMM:NF016754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV2583778.1"
                     /translation="MPVLGLTTVLTSLDSVQASMLKQVGDAVQNAGIVTEAVAKQRTR
                     VRTGRLRAGNRYVKTGERSCRIGNAVRYAAAVELGHRVRGKSGRTVPPRPFLFPGYLA
                     GKQALKEDLAKIPGLRIRSWS"
     gene            466..780
                     /locus_tag="VGT44_23185"
     CDS             466..780
                     /locus_tag="VGT44_23185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583779.1"
                     /translation="MAQGHIRLLKAAWDKTIPTTLPDWQKALKLPDSYRLVEVRESAC
                     GMYPNWLFVVENDLIPETTGRVKPDIVPIYRKDAGGHVTLDHIETRVWDGSAWCSLDE
                     VA"
     gene            780..1241
                     /locus_tag="VGT44_23190"
     CDS             780..1241
                     /locus_tag="VGT44_23190"
                     /inference="COORDINATES: protein motif:HMM:NF022810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HEV2583780.1"
                     /translation="MALPNGAIMNALITRYRADTALATLLGNPTNPPGAIFDGGGGVP
                     TNQPFPYIAVYIPTSQSGTTLAMDQDAVDSYVQVSVFTQPGAGGGMKQARDIIERVYA
                     LTQPPAPPLDLSASGFAQFFLLFENEQEVPPNQDGLTPMIAHRYHLMTQTL"
     gene            1265..1678
                     /locus_tag="VGT44_23195"
     CDS             1265..1678
                     /locus_tag="VGT44_23195"
                     /inference="COORDINATES: protein motif:HMM:NF017961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HEV2583781.1"
                     /translation="MTAVPTQGFLGFVKVGTSASPTNKVAGVSDISLPFAAAEYDVSN
                     MDVANNGWQQYIPGLKGAKNALKVWYDPTDTNGQLVLQNAWVNGTLLYFIVSINGTNT
                     ATFTAYVSDMSQHGPVNNANEATYTLQMTGSVTFA"
     gene            1693..2661
                     /locus_tag="VGT44_23200"
     CDS             1693..2661
                     /locus_tag="VGT44_23200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583782.1"
                     /translation="MTDHTAVPLPGYKCLVNLSSTPSVSSSDLALTDAGDHQTFTVPL
                     ASAQRYLDRAVAVTVQTQQDEIQTVTITGSPTGGTFTLTFGGNTTTGIAYNAAASAVQ
                     SALTALASIGANNVSVSGSNGGPWTVEFIGSLKDASQSLMTGNGGSLTGGSSPNVSIA
                     RTQAGQAWTTISSSSYTLRYLTAQVLLNAALLGSNIGLRLHAFNYYPYASIALANDIT
                     FAGQRKMLDVTALQGSSGGGWQSFIPGLNGGTFACKAWVYSAGAALYASNLASNALLI
                     LSFVDPTGNHAFESYGYTKGDTWHGAVANANEETINFQCDAIVALS"
     gene            2683..3183
                     /locus_tag="VGT44_23205"
     CDS             2683..3183
                     /locus_tag="VGT44_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583783.1"
                     /translation="MPEIVDRLSARQFLLGDDFRPREENIPTPQLLLDKGFPAESLRL
                     VGFPADKAKQLSDTSNGNQVLYVALCTCACLCWRADGQPVFNMADAPMLACERDYGLL
                     NALAVPVKPFLGIGPAAVDNAKNDSEVQTKGAAGPKNVGGTASPNDSNFPPSEPASEP
                     SNEQTS"
     gene            3260..3460
                     /locus_tag="VGT44_23210"
     CDS             3260..3460
                     /locus_tag="VGT44_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583784.1"
                     /translation="MKRPPSQRVAIRCQVSHNLLAYRDERGIYLFCKECRTEQIFAWS
                     EIVPRSLAASEIRPALDAKPAP"
BASE COUNT          796 a         1027 c         1055 g          733 t
ORIGIN      
        1 gagaatccac tcgaagccaa tgtctcgatg tggctcttgt gccaggagaa ccaggcgcag
       61 gcagtgaact gaacaaggaa ggagtggcgc aatgcccgtg ttaggactta cgaccgttct
      121 cacgagcctc gacagcgtgc aggcatcgat gctcaagcag gtcggcgatg ctgtgcagaa
      181 tgccgggatc gtcaccgagg ccgtcgccaa acagcgaacg cgtgtacgga ccggacgact
      241 gcgagccggg aatcggtacg tgaagacagg cgaacgctcc tgccggattg ggaacgccgt
      301 ccgatacgca gctgctgtgg agctgggaca tcgggtccgt gggaagagtg ggcgcacggt
      361 tccaccccga cctttcctgt tccccggcta tctggctggc aagcaggcac tcaaagagga
      421 tctcgcaaag attcctggtc tgcgcattcg gtcttggagt tgatcatggc acaagggcat
      481 atccgtttac taaaagcggc ctgggataaa accataccga caacacttcc cgattggcag
      541 aaagcgctga agctcccaga cagctaccgg ctggtggagg ttcgggagtc ggcctgtgga
      601 atgtatccaa actggctgtt tgtcgtggag aacgacctga ttccagagac gacgggacgt
      661 gtgaagccag acattgtgcc gatctaccgc aaggatgccg gtggccatgt cacgcttgat
      721 catatcgaga ccagggtctg ggatggctcc gcatggtgct cgctggatga ggtggcttga
      781 tggcactacc gaacggcgcg attatgaatg cgctcattac tcgctaccgc gcggatacgg
      841 cgctggcgac cttactcggc aacccgacga atccaccggg agcgatcttt gacggtgggg
      901 gcggcgtgcc gacaaaccag ccgtttccgt atatcgcggt ttacataccg acctcacaat
      961 cagggacaac cctggcaatg gatcaggacg cagtagacag ctacgtccag gtgtcggtct
     1021 ttacgcagcc aggcgcgggc ggcgggatga agcaggcgag agacatcatc gagcgtgttt
     1081 atgcgcttac acaaccacct gcgccgccgc tcgatctctc agctagcgga tttgcacagt
     1141 tcttcttgct cttcgagaac gagcaggaag tgccgcccaa ccaggacggc ttaacaccaa
     1201 tgatcgcgca tcgttatcat ctgatgacac aaactttgta gtttagaaga aaggcagtga
     1261 ttttatgacc gccgttccaa cacagggctt ccttggcttt gttaaagtcg ggaccagcgc
     1321 gagcccaact aataaagtcg ccggggtcag cgatatttcg ctgcctttcg cggccgctga
     1381 gtatgacgtg agtaacatgg atgttgccaa caacggctgg cagcaataca ttcctggcct
     1441 aaaaggtgcc aaaaacgcgc ttaaagtctg gtatgacccg actgacacaa acgggcaact
     1501 cgttctgcaa aatgcctggg tcaatggaac gctgctctac ttcatcgtga gcatcaacgg
     1561 gaccaacaca gccacgttca cggcctacgt cagcgacatg agccagcacg gccccgtgaa
     1621 taatgccaac gaagcgacct acacgctcca gatgaccggc agtgtgacgt tcgcctaaga
     1681 taggaggctg ccatgaccga tcatactgcg gtgccactgc ctggctataa gtgcctggtc
     1741 aatctcagct cgacgcccag tgtgtcgtca tccgacctgg cgctgactga tgctggcgac
     1801 caccagacgt ttactgtgcc gttggccagt gctcaacgct acctggatcg agccgtggcc
     1861 gtcaccgtcc agacccagca ggacgagatc cagaccgtga cgataacggg gtcgcccact
     1921 gggggcacgt ttacgttgac ctttgggggc aacaccacaa ctggcattgc gtacaacgcc
     1981 gcagccagcg cggtgcagtc tgcgctgacc gcgctggcta gcatcggggc caataacgtc
     2041 agcgtgagcg gcagcaatgg cgggccgtgg acggtcgagt tcatcggcag cctgaaagat
     2101 gcctcacaga gcctcatgac ggggaacggc gggagtctga ctggtggcag ttcgccgaat
     2161 gtgtcgattg cccgtaccca ggctggacag gcctggacaa cgattagcag ctcctcctac
     2221 acactccgct atctgaccgc gcaagtgctg ctcaacgctg ccttgctcgg gtccaatatc
     2281 ggactgaggc tgcacgcctt caactactat ccctatgcct cgattgcgct ggccaatgac
     2341 atcactttcg cgggacagcg gaagatgctg gacgtgactg cgctccaggg ctctagtgga
     2401 ggcggctggc agagcttcat ccctggcctc aacggcggga cgttcgcctg caaggcctgg
     2461 gtttatagcg ccggagctgc tctctacgcg agcaatctgg ccagcaatgc tttgctgatc
     2521 ttgtcgttcg tggatccgac ggggaaccac gcgttcgagt cgtatggcta caccaagggc
     2581 gatacctggc atggcgcagt ggctaacgcg aacgaagaaa ctatcaattt ccagtgtgat
     2641 gcaattgtcg cactcagtta aatcaagaaa ggcatgtgtg gtatgcccga aatcgtcgat
     2701 cgtctcagcg cgcgccagtt cctgcttggc gacgacttca gaccgagaga agagaatatt
     2761 ccgacgccgc aactcctgct cgacaaaggc ttcccggccg agagcttgcg cctggtcggt
     2821 tttcctgccg acaaggctaa gcagcttagc gatacatcca acgggaacca ggtgctctat
     2881 gtcgcgctct gcacctgtgc ctgtctctgc tggcgcgcgg acggccagcc agtcttcaat
     2941 atggcggatg caccgatgct cgcttgtgag cgtgattacg gcttgttgaa tgctctggca
     3001 gtccccgtca agcccttttt gggtatcggc cccgcagcag ttgataacgc aaaaaacgac
     3061 tccgaggtcc agacgaaggg agcggctgga ccgaagaacg ttggtggtac cgcgtcgccg
     3121 aacgattcaa atttcccacc gtccgagcct gccagcgagc catcgaacga gcagacttcg
     3181 taaactgggt ggcctatctc cagcttcagg atgaggatga gcacgaacgg ctggtcgcca
     3241 tgatgggagg acagagatca tgaagcgtcc gccatcacag cgagtcgcca ttcgctgtca
     3301 ggtcagtcat aacttgcttg cgtatcggga cgagcgcggg atttatctct tctgcaagga
     3361 atgcagaacc gaacaaattt tcgcctggtc ggagatcgtg ccgcgatcgc tcgcggcttc
     3421 cgagatccga ccagccctgg atgcgaagcc cgcaccctaa caattgcata ccgatatctt
     3481 tgagcctttg agccagatcc cctacgggat ctggcttttt tcatcgattg gaacatcttt
     3541 tgagcgatct gggcacactc agcgtcggct ttgaagtcga tgttaatagc ctgagcagcg
     3601 gatttgatac c
//
LOCUS       DASZAK010001326         2920 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_209965, whole genome shotgun sequence.
ACCESSION   DASZAK010001326 DASZAK010000000
VERSION     DASZAK010001326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209965"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VGT44_23215"
     CDS             <1..468
                     /locus_tag="VGT44_23215"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2583785.1"
                     /translation="VIESDEATWERTFAVHVRGAFLCSKYTLPAMIDSGHGGVVINVS
                     SVAGLVGLQRMSAYSAAKGAIISLTRAMAADFAQYGIRVNCIAPGTTMTPLGQRLIEN
                     DTPERLAQRLSRYPLGRFGQPEEIARSVLFLASDDSSYATGMCLVIDGGLTSV"
     gene            483..1241
                     /locus_tag="VGT44_23220"
     CDS             483..1241
                     /locus_tag="VGT44_23220"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2583786.1"
                     /translation="MRLANKIALVTGAGSGIGRGIAHRFAAEGAFLVLSDIADAGLQE
                     TVAMIEEAGGRSVSVVGDVAVRADAERMVEAAATHWGRLDIAVNNAGITGSRQATLAH
                     LTPDEEWERVMAVNVSGVFRVASAALRQMLEQGSGTIINIASAAGLVPFPARAAYNAS
                     KGAVISFTRALALDYAPNHIRVNAICPGMVETAMTRWRLEIPELRQQVLDMTPWGRIG
                     QPEDIAAAAVYLASDEADFVTGHMLVIDGGWIIK"
     gene            1276..2223
                     /locus_tag="VGT44_23225"
     CDS             1276..2223
                     /locus_tag="VGT44_23225"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEV2583787.1"
                     /translation="MSIFTSEDKRMELSEAAAIVQDGMTIAIGGGLSWREPMTFLREL
                     IRQERRNLRVVGTAHGVDVDLLCGAGAISVVEESYVGFEQDFGMAPNFRRVCESGRVQ
                     VHDTCCHTIIQQLRAAEFGVPFLPVRSVQGTDFLKLHPEYKTMTCPFTGQPLVLVPAL
                     SPDAAIVHAQYGDAQGNLKILPPLVADLFFIRASKHVIASVEKLLPTDELRAMEPNVP
                     YFWVESVVEVPYGAHPTSCYPFYAYDRAHLTEYYQAARAGQDTFANEYLARYVFGPAN
                     HDEYLSRIGGQTKRQALESWKDGDEAWRALFTAPEGAIA"
     gene            2220..>2920
                     /locus_tag="VGT44_23230"
     CDS             2220..>2920
                     /locus_tag="VGT44_23230"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEV2583788.1"
                     /translation="MNVEPITYATLPEVMIYQIARTIQDGELVFHGYGSPVPTLAMLL
                     ARRTHAPRMVHIEGATYGVNPEPPFLPPTSNDWSLEQGSVMALGISDLFDIAARGDMG
                     RMFLSGVQIDRYGNLNVTAIGDPDSPRIKLPGSGGGCNLSGDVQHITIWTTGHRAPIS
                     SAGKRQFRFVKRIDFISSIGHVSRDGISRQALGLRGGGPDWVVTELGVFDFDPVTHVL
                     RLRALYPDTSVQDVL"
BASE COUNT          600 a          795 c          863 g          662 t
ORIGIN      
        1 gtcatcgagt ccgatgaagc aacctgggag cgcacattcg ccgtccatgt gcgcggtgct
       61 ttcctgtgtt cgaagtacac tcttccggcg atgattgaca gtggacacgg aggtgtggtc
      121 attaatgttt cgtcggttgc cggtctcgtt ggattgcagc gcatgtccgc ctactccgca
      181 gccaagggag ctatcatcag tctgacacgc gcaatggctg ccgattttgc gcagtatggc
      241 atccgcgtga attgcatcgc tccaggtaca acgatgacgc cgttgggtca acgcttgatt
      301 gagaacgata caccagagag gctggctcaa aggctgagtc gctacccact tggtcgtttt
      361 ggacagcctg aagaaatcgc acgttctgta ctgttcttag cttcggatga tagctcgtat
      421 gcgacaggca tgtgcctggt tattgatggt ggattaacca gcgtttaggg aagccattga
      481 ctatgcgtct tgcgaataaa attgccctgg tcaccggtgc aggctccggc attgggcgcg
      541 gaatcgccca ccgcttcgct gctgaagggg cattcctggt gctcagtgac attgctgacg
      601 cagggttgca ggagacggtg gcaatgatcg aagaggcggg gggtcgatct gtttctgtgg
      661 tgggcgacgt ggcagtacgg gcggatgcgg agcggatggt cgaagctgca gccacgcact
      721 gggggcgact ggatatagcg gtaaacaatg ccggtatcac cggctcgcgc caagccacac
      781 tcgctcacct gactcccgac gaagaatggg aacgcgtgat ggcggtcaat gtgagcggcg
      841 tgttccgggt cgccagtgct gccctccggc agatgttgga acagggaagt ggcaccatca
      901 tcaatattgc atcagccgcc gggcttgtgc cttttccagc acgggccgct tacaatgctt
      961 cgaaaggggc ggttatttca ttcacacgtg cgctggcgct ggactatgca cccaaccaca
     1021 tccgggtcaa tgctatctgc cccggcatgg ttgagaccgc tatgacacgc tggcggctgg
     1081 agattccaga actgcgccag caggtgttgg atatgacccc ctggggtcgt atcggccagc
     1141 cggaagacat cgctgctgct gccgtttacc ttgcttcgga cgaagccgat ttcgtcaccg
     1201 gacacatgct ggttatagat ggcggctgga taatcaaata aaattcccca tatcattcga
     1261 gaataggggt aagctatgtc gatctttact tcagaagata agcggatgga gctttccgag
     1321 gccgcagcta tcgtgcaaga tggcatgacc attgcgattg gcggcggcct gtcctggcgc
     1381 gaaccgatga cctttttgcg cgaacttatt cgacaggagc gacgcaatct gcgcgtggtg
     1441 ggaacggcgc acggtgtgga tgtggatctt ctctgcggcg cgggcgctat tagcgtggta
     1501 gaggagtcat acgttggctt cgagcaggat tttgggatgg ccccgaactt ccgccgcgtg
     1561 tgtgaaagtg ggcgcgtaca agttcatgat acctgctgtc ataccatcat tcagcagttg
     1621 cgagcagccg agttcggtgt cccatttcta ccagtgcgga gcgtgcaggg cacagatttt
     1681 ctgaaactgc acccggaata caagaccatg acctgtccat ttacagggca gccactggtg
     1741 cttgtgcccg cgctctcgcc agatgctgcc atcgtgcacg cgcagtatgg tgatgcacag
     1801 ggcaatttga agatactgcc accgctggtc gctgacctct ttttcatccg cgcctctaaa
     1861 catgtaatcg catctgtcga gaaattattg ccaacggacg aactgcgggc gatggaacca
     1921 aacgtgccgt atttctgggt cgagtcagta gttgaggttc cttacggcgc tcatcccact
     1981 tcatgctacc cattctatgc ctatgaccgt gcgcacctga ctgagtacta ccaggcagcg
     2041 cgggcagggc aggacacttt cgcaaatgag tacctggcac gttacgtgtt tgggcctgca
     2101 aaccacgacg agtacctttc ccgtatcggc gggcagacaa aacgccaggc gcttgaaagc
     2161 tggaaggatg gcgatgaagc ctggcgtgcc ctcttcaccg cacctgaagg agccatcgca
     2221 tgaacgtcga accaattact tatgcaacac tccctgaagt gatgatctac cagatcgcac
     2281 gcactatcca ggatggtgag ttagttttcc acggctatgg ttcaccagtg ccgacgctcg
     2341 ccatgctctt agccaggcga acccatgcgc ccaggatggt gcacattgag ggtgccacct
     2401 atggtgtcaa ccccgagcca ccatttctac cgccgacctc caatgactgg tcactggaac
     2461 aagggagtgt aatggcgctc ggtatcagcg acctgttcga catagcagcc cgtggcgata
     2521 tggggcgcat gttcctctcc ggcgtgcaga tcgatcgcta tggcaacctg aacgtcacag
     2581 cgattggcga tccggattct ccacggatca agctacctgg cagcggcggc ggctgcaacc
     2641 tgtctggcga tgtccagcac atcaccatct ggactacggg gcatcgcgcg ccaataagca
     2701 gcgctggcaa gcgccagttt cgtttcgtca agcgcataga ttttatctca tccatcggcc
     2761 acgtctctcg tgacggtatc tcgcggcagg ccttgggcct gcggggcggt ggaccagact
     2821 gggtcgtgac cgagttaggt gtctttgatt ttgatccagt tacgcacgtc ttacgcctgc
     2881 gtgcactgta tcctgatact agcgtccagg acgtgctgga
//
LOCUS       DASZAK010001327         2904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_425340, whole genome shotgun sequence.
ACCESSION   DASZAK010001327 DASZAK010000000
VERSION     DASZAK010001327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425340"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            525..674
                     /locus_tag="VGT44_23235"
     CDS             525..674
                     /locus_tag="VGT44_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583789.1"
                     /translation="MPESRVLQQLTIEQTQLLTQPRASRQGALHIKAIRLAGIEQQVE
                     ASLDD"
     gene            1176..2489
                     /locus_tag="VGT44_23240"
     CDS             1176..2489
                     /locus_tag="VGT44_23240"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583790.1"
                     /translation="MSTQPLFRATGELVNAAGQQPALLLREPLPARTRRLLWLLSASL
                     ALMMSGYGIVLPVFAKRLGELGSGVAALGLMSMGFAFAQFLLSPFLGSVADRIGRKPL
                     ILVALAGEVVANSAFLLASSTGWYLAIRLFQGAIEAGLLPAVLGVVADSVPESGRARW
                     TGIVMGSYGAGFVFGPTLGGVLFDRWGFAAPFGVSAVLALIALVMALVMVPETRSVLG
                     SRQERVASRGRGRLLASLPQPLYLFATLLVLDFIATFGFAFIEPQMMFYLYNQVLLTP
                     TQLGIIVGGYGLAMLIGQATLGQSSDRYGRKPLIVLGLLLSVMFYLSLIFLRSFGWLF
                     LGALVTGIGGALLSPALSAAYLDIAAPEHSSQVMGIRGASASLGGVLGPLLVAVVSLW
                     IAPQGIFAIAGGVTLGAVVLAFLVLKGGTRRRGASAQSVLMNELG"
     gene            complement(2711..2904)
                     /locus_tag="VGT44_23245"
                     /pseudo
     CDS             complement(2711..2904)
                     /locus_tag="VGT44_23245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933337.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          577 a          784 c          889 g          654 t
ORIGIN      
        1 ctcgctaccc cgcgagcgta tagatggcaa tgcaaaggat tgaggagcgc catcagtgat
       61 gctctttttt ggaacggatc tcctgcgctt ccagtatcag gaagaccgaa ttcaaattca
      121 cgacagcctg cttggaaggc agggtgtccc aggatcgccg gtcgcctggt atgaatcgtc
      181 tttagagggg tgactctatt ttttcgggca agacatacgc taagcccacc gcattccctt
      241 aaggcgcatg cttttcactc cttgaaatgg aaaagatgca gatatccctc gcttttgaat
      301 ggtgatcttt cgcaaggaga agcagataga acagcccgag aaggcatctc atctcttaac
      361 ggaagattct cccggaaaaa tatggtcacc ccgttgtgtt ttgtcatagc gatcggtgac
      421 gtagacacgc tcattgtttt ccttgaccca ggcgtagatg tgcttgacgg gcatccgcca
      481 ccgtttcccc caagccagcg atttcggctt gcagagcgcg gacggtgccc gaaagccggg
      541 tcttgcagca gctcacgatc gagcagacgc agcttctcac ccagccaaga gcgagtcggc
      601 agggtgcgct ccacatcaaa gccatcaggt tggctgggat cgagcagcag gtggaagcct
      661 cactcgatga ctaacagcgg atgcttcagg agaaactcgc gcagttgggt ggggtcactc
      721 aagccgtact tgatgcggat caagaaggct ttgagataag ccctttcggg atgagcaggc
      781 cgcccgcgag aggaacgcgc agcaggccct cgctcaatca cagaccaatc gaagcaggaa
      841 aagaaggctc gagagttggt gaccagcgga tcggcatcaa gcagagcggc gatgagagac
      901 tggtcaaaga ggtgagaagc gggtatcaga gcagagaact cctttggagg tggaaggggt
      961 tttcatgaag actcctctgc aaaaggaatt cttgctgggt ctcacccatc ggcttttgtc
     1021 tcatcccaaa tcacccatgt gggaaatgac gcgaactggt tgcgaaacgc ttgatgaggt
     1081 atactagtgg agtgaaggct ttcaagaggc ctgatcgagc agccaagtct ggacctgctt
     1141 gcgtcgagcg agagcaaagc acaaggaaga agcgcatgag tacacaaccc ctctttcgcg
     1201 ccacgggcga gttagtcaac gcggctggac aacagccagc actcctctta cgagaaccac
     1261 tcccagctcg gacacgcagg ttgctctggc tgctgtcagc cagtcttgcg ctgatgatga
     1321 gcggttacgg gattgtcctg ccggtgtttg ccaagcggct gggtgaactt ggctcaggcg
     1381 tggcggcact ggggctcatg tcgatggggt tcgcgttcgc gcagttcttg ctctccccgt
     1441 ttctgggctc agtcgccgat cgcatcggca gaaagccatt gatcctggtg gcgctggccg
     1501 gcgaggtggt tgccaatagc gcgtttctcc tggcttcgtc aaccgggtgg tatctcgcga
     1561 tccgcctgtt tcagggagcg attgaggcgg gactgctccc ggcggtcctg ggcgtggtgg
     1621 ccgatagcgt accggagtct gggcgggcca gatggacggg catcgtcatg ggcagctatg
     1681 gggcaggctt cgtcttcggc ccgacgctgg gaggggtcct ctttgaccgc tggggcttcg
     1741 ctgccccctt tggggtctct gccgtcctgg ccctgatcgc cctggtgatg gcgttggtga
     1801 tggttccaga gacgcgctca gtcctgggga gtcggcaaga gcgggttgcg agcagaggac
     1861 gcgggagact actggcatcc ctgccccaac cattgtacct cttcgccacg ctgctggtgc
     1921 tcgatttcat tgccaccttt ggctttgcct tcatcgaacc gcagatgatg ttctacctgt
     1981 acaaccaggt gctcttaacg cccacgcaac tggggatcat cgtgggaggg tacgggttgg
     2041 ccatgctgat cggacaggca acactgggcc aatcgagtga ccggtatggc agaaagccgc
     2101 tcatcgtgct cggcctgctc ttgagcgtca tgttctatct cagtttgatc ttcctccggt
     2161 cgtttggttg gctcttcctg ggagctctgg tcacgggaat agggggtgcg ctgctgtcac
     2221 cggcattgag tgcggcttac ctggatatag cggcaccaga acatagctcc caggtgatgg
     2281 gcattcgggg cgccagtgct tcacttggtg gcgttctcgg tccgctcctg gtggccgtgg
     2341 tcagcctctg gatagccccg caaggcatct ttgccattgc aggaggggtg acgctgggtg
     2401 cagtcgtgct ggctttcctg gtgttgaaag ggggtacacg ccgtcgtgga gcatcagcgc
     2461 agtcggtgct catgaacgag ctcggctgag ccgagttcgc caacaagggg tgaccatagt
     2521 atctgggcaa aacctccgtt aagcaacaaa gccactcctc aggctcgctc atgagagcgg
     2581 ccaaggggtc aattccctcc gtatctctcc tctcgcatcc gccttttctt ctcttgtgct
     2641 gcaaatgaag gagctagaag acctctcagt gtccttagcg tatgtttctc cccggagaat
     2701 atggttaccc tacattgatt gccgcggcaa tgccaacatg ttgcaaatgg gtcttggctt
     2761 cgccaatgta acgagaacgg cgcatgccaa aggcgtgcac cccttgagag atggtggctt
     2821 cgatgccgga gcgggccgca tattgcctac taaaggtttc tgtggtctgt cgatgacgcg
     2881 ctgcctgcaa ggcctggtac tgct
//
LOCUS       DASZAK010001328         1893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_532218, whole genome shotgun sequence.
ACCESSION   DASZAK010001328 DASZAK010000000
VERSION     DASZAK010001328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532218"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VGT44_23250"
     CDS             <1..723
                     /locus_tag="VGT44_23250"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV2583791.1"
                     /translation="PYLLQPGVGAAFGLPCYSNWRTSWSSLVSGLVNANMLLSFVALT
                     YLTEPFRITGHMAAFRRDSFTEVGGLDGLEQHIDDDFELARRLHSHGLRSVQAPLVYD
                     IDNDLPSPAAYTAQLRRWFVLPRQAMMPSLTPWQRAIAFLTSAALPLPTISALLALLT
                     CRRTAVLSLGASLGVFAMVYALCEARYLKGRTPPARWPMLMVMALLTPLQIARALLSN
                     NVVEWRGQRLRVLRNGTVEVTS"
     gene            720..>1893
                     /locus_tag="VGT44_23255"
     CDS             720..>1893
                     /locus_tag="VGT44_23255"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2583792.1"
                     /translation="MKKLGLAFALLMLTERAWKYRMVERFFRQPIPDPIADPTLVSIL
                     QPVLSGDATLAACLERSLQLKSRYPLEVIWLADEDDSEGQRICRELMGRYPERDARLI
                     ALPPPQEGQNPKTVKLIAGAKVARGEVICVLDDDTMLPDNGLENCLPFLDQPGVGLSF
                     GLPYYVNFASTWSSLVSCFVNSNSLLTYIPYTALTQPFTINGMFYTMRRETLASVGGF
                     EAIERVFADDFAVAWLLRSHGYQLAQSPLLHGISTTVGGPRHYLSLMRRWFVLPRESL
                     LRHLGWYDRTIIYLLGLVPAFFPLLLVLSLLLRPSRRKLGFALLYSGYSFSIFARLNA
                     RYLRNATPWSKVWWIPVVQLIFPAQLLIALLSPQHINWRGNIIQVERGGEFHFIRQR"
BASE COUNT          313 a          649 c          532 g          399 t
ORIGIN      
        1 ccctacctgc tacagcctgg cgtgggcgct gcctttggtc tgccctgcta ttccaactgg
       61 cgcacatcct ggtcgagcct ggtgagcggc ctggttaacg ccaacatgct gctgagcttt
      121 gtagcgctca cctacctgac cgaaccgttc aggatcaccg gccacatggc tgccttccgc
      181 cgagacagct tcacggaagt aggtggactg gatggcctgg aacagcacat cgatgatgat
      241 ttcgagctgg cgcggcggct gcattcgcat ggattgcgat ctgtacaagc gccactggtg
      301 tatgatatcg ataatgacct gccttcacca gccgcgtata cggcgcaact ccgacgctgg
      361 tttgtgctgc cgcgccaggc catgatgccc tcgctcacgc cctggcagcg cgcgatcgcc
      421 ttcctcacca gcgccgcgct gccgttgccg accatcagcg cgctgctggc tctgctgaca
      481 tgccgccgga ctgcagttct gtcgctgggc gcaagtctcg gcgtcttcgc gatggtctac
      541 gcgctatgcg aggcgcgcta tctcaagggg cgtactcccc cggcacgctg gccaatgctg
      601 atggtgatgg cgcttctcac accgctgcaa atcgccaggg cgctgctctc caataacgtt
      661 gttgaatggc gcggccagcg cctgcgcgtc ttgcgcaacg gcacagtgga ggtgacatca
      721 tgaagaaact cggcctggcg tttgccctgc tgatgctcac cgaacgcgcc tggaaatacc
      781 gcatggtgga acgtttcttc cgccaaccca ttcctgatcc gatcgccgat cccacgcttg
      841 tgagcatcct tcagcccgtc ttgagcggcg acgcgaccct tgcagcctgc cttgaacgca
      901 gcctccaact caagagccgt tacccgctcg aggtgatctg gctggccgac gaagacgata
      961 gcgaaggcca gcgtatttgc cgcgagctga tggggcgcta cccggaacgc gatgcgcgcc
     1021 tgatcgcact tccaccgccg caggagggac agaatcccaa aaccgtcaag ctcatcgcgg
     1081 gcgcgaaggt cgccaggggc gaggtaatct gcgttctcga cgacgatacg atgcttcccg
     1141 acaatggcct ggaaaactgc ctgcccttcc tcgatcagcc cggcgtcggc ctgtccttcg
     1201 gcctgccgta ctacgtcaac tttgccagta catggtcaag cctggtctcg tgcttcgtca
     1261 acagcaacag cctcctgacc tacatccctt ataccgcgct cacccagccc ttcacgatca
     1321 acggcatgtt ctacaccatg cggcgggaga cgctcgccag cgtgggcggc tttgaggcca
     1381 tcgagcgcgt cttcgccgac gatttcgcgg ttgcctggct gctccgttcg catggctatc
     1441 agctcgcgca gtctccactg ctgcacggca tcagcacgac ggtcgggggg ccgcgccact
     1501 acctgagcct catgcggcgc tggttcgtgc tgccccgcga atcgctgctg cgccacctgg
     1561 gctggtacga ccgcaccatc atctatctcc tggggttggt cccggcgttc ttccccctgc
     1621 tgctggtcct ttcgctgctc ttgcgcccct cgcgccgcaa actggggttc gcgctgctct
     1681 attccggcta cagcttctcc atcttcgccc gcctgaacgc ccgctacctg cgcaatgcaa
     1741 caccctggtc gaaagtctgg tggatacccg tcgtccagct catctttcct gcgcagttgc
     1801 tgatcgcgct gctctccccg cagcacatca actggcgcgg caacattatt caggttgagc
     1861 gcggcggcga atttcacttt attcgccagc gat
//
LOCUS       DASZAK010001329         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_33988, whole genome shotgun sequence.
ACCESSION   DASZAK010001329 DASZAK010000000
VERSION     DASZAK010001329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33988"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VGT44_23260"
     CDS             <1..749
                     /locus_tag="VGT44_23260"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2583793.1"
                     /translation="TPPAQDTSAQPAQPQAQPPVQDTVLRCPNCGNELPKVATFCPKC
                     GKPLSGQPPRNLQGQSSAPPSQQNRGANNANNPKNMSSEPTMIYAPSQSPTLRRKSPT
                     GPTGAGQPGGMPQAPPSFTPGTQPDQMRQARQMEPPASQFSARALPAQVAQPAMPRPF
                     LQTMTPSSRSAPDGAPSFQISQEDRHAQSISAPPATPSPNSNHLVSPAGIAGGTSTQK
                     LVLYAIFAFGAILVIILILLVSHLGAGHSG"
     gene            802..1872
                     /locus_tag="VGT44_23265"
     CDS             802..1872
                     /locus_tag="VGT44_23265"
                     /inference="COORDINATES: protein motif:HMM:NF012945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583794.1"
                     /translation="MNETSLSELLPSLRIVLLGYGHVARAFMPLLASRHEWIGRELGL
                     RPVIVGLGSRSIGLYTHTDGINAHELAQEVESLNRFRQAGIRANTVEQFIEAGKAAGA
                     TLFIELTTLNPLDGQPALAHIRTALELGMDVITANKGPVAFAQVELQSLARRQQVQFR
                     FESCVMDGLPLLNLAEFTLPAVEMSGFRALLNSTSSLVLGMIEQGYAREEALLKAQQM
                     GISEVDPSFDLDGWDAVMKTTILANTLLEGNLTPRMVEREGIRHLTSDDICSAALAGS
                     PYRLVSEAHRAQGVIRAVVTPQRIQTDDILHAAINLGGIISLETEAMGILSIVEHSNN
                     VTQTAYGVLSDLVNIERNRRAG"
     gene            1893..2816
                     /locus_tag="VGT44_23270"
     CDS             1893..2816
                     /locus_tag="VGT44_23270"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEV2583795.1"
                     /translation="MDTSNLDIIGIGGAGGLIASSLMRGTLTPRIFARGSALKQLRDV
                     GLTIITDQHRAIFAPISCSALDEVATLASIVCIATKSYDIPVLLETIATKLSEDTLFI
                     VVQNGFAAYDMVSAAVGAERVAMGVLYVGAQIISPAVIDIKPGIAQLFLPARHRKCLG
                     RVVDALESGGMEAALVDDIERRMWMKQLFLVPFAIVNTEMRQPIGKVYQNAAARERWG
                     DIAREMAAVATACGIPMPTDPAAASLAVAERFDPQADSSFARDIWANRPHEAEALFGP
                     PLQRAAAHGVPCPLLQEAYERFGKRQGLSAC"
     gene            complement(2996..>3178)
                     /locus_tag="VGT44_23275"
     CDS             complement(2996..>3178)
                     /locus_tag="VGT44_23275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2583796.1"
                     /translation="PDVPIEEVAGAVKDLFEEGKVKHFGLSEAGAGTIRRARLVACSE
                     AVDRSHTWLPQARAPG"
BASE COUNT          676 a         1005 c          862 g          635 t
ORIGIN      
        1 gtacgccgcc cgcgcaggat acgtcggctc aacctgcaca gccacaagca cagcctcctg
       61 tacaggatac cgtgctacgc tgcccaaact gcggaaatga gctgccgaaa gttgccacgt
      121 tctgtccgaa atgcggtaag ccattgagcg gtcagccccc tcgcaacttg caggggcaga
      181 gcagcgcgcc tccctcgcag caaaacagag gcgcgaacaa tgcgaataat ccgaagaaca
      241 tgtcatcaga gccgacgatg atctacgcgc cttcgcagtc accaaccctg cgtcgcaaat
      301 ctccaaccgg cccaacaggc gccggacagc caggaggaat gccacaagcc cctcccagct
      361 tcacaccagg cacgcagcct gatcagatga ggcaggcgcg acaaatggaa ccgcccgcct
      421 cgcaattttc cgcgcgggca cttccggcgc aggtcgcgca accggccatg cccagaccat
      481 ttctccaaac tatgactcca tcgtcccggt cagcacccga cggcgcacca tcctttcaaa
      541 tttcgcagga ggaccgccat gcacaatcca tttcagctcc tcctgctact ccttcaccca
      601 atagcaatca tctcgtttcg ccagcaggca tagccggtgg aacttcgaca caaaaactgg
      661 tgttgtatgc catttttgcg tttggggcaa ttttggtgat tatcctgatt ctgctcgtat
      721 cccatctcgg cgcaggacac tcaggataac gctacttcat atcgggtgga gacttcatca
      781 tcctggcaaa gggacacaac aatgaacgaa acttcgcttt ccgagctgtt gccctcgctg
      841 cgcatcgtgc tgctgggata cggccacgtg gcgcgcgcct tcatgcccct gctggcatcg
      901 cgccacgaat ggatcgggcg cgagttgggc ttgcgacccg tcatcgtcgg cctcggctcg
      961 cgtagcattg gtctctatac ccatactgat ggcattaatg cgcatgaact tgcgcaagaa
     1021 gtagaatcgc tgaacaggtt caggcaagct ggaatccgcg caaacacggt cgaacaattc
     1081 atagaagccg ggaaagcagc cggagctacg ctcttcatcg aattgaccac ccttaatccc
     1141 ctggatggtc agcccgccct cgcacatatc cgaacagccc tggaactcgg tatggatgtc
     1201 atcaccgcca ataaaggccc ggttgctttc gcgcaggtcg agttgcaatc actggcgcgt
     1261 cgccagcagg tgcagttccg cttcgaatca tgcgtgatgg atggtctgcc cctgctcaac
     1321 ctggccgagt tcacactgcc cgccgtcgag atgagcggat tcagggcgtt gctgaactct
     1381 accagcagcc tcgtcctcgg catgatcgag cagggctacg cgcgagagga ggcgctgctc
     1441 aaggcgcagc agatgggtat tagcgaggtc gatccctcgt ttgatctgga cggctgggac
     1501 gcggtaatga aaacaactat cctggccaat acgctgctgg aaggcaacct gacgccccgc
     1561 atggtcgaac gcgagggcat tcgccacctc accagcgatg atatctgctc ggcggcgctg
     1621 gccggttcgc cctatcgcct ggtcagcgaa gcccaccggg cgcagggagt catacgtgca
     1681 gtggtgacgc cgcagcgcat ccagacagac gatattctgc acgcggcaat aaatctgggc
     1741 ggcatcatct cgctagaaac cgaggcaatg ggtatactat caatagtgga acatagcaac
     1801 aacgtcaccc agacagcgta tggcgtgttg agcgacctgg taaatatcga acgcaatcgg
     1861 agagcaggat agcctctctg gagggctaaa gtatggacac gagcaatctt gacatcatcg
     1921 gtatcggcgg agctggaggc ttgatcgcat ccagcctgat gcgcggcacc ctgactccac
     1981 gcattttcgc gcgtggatca gcgctcaagc aacttcgcga cgttggatta accatcatca
     2041 ccgatcaaca ccgggctata ttcgccccga tctcttgctc agcacttgat gaagtagcaa
     2101 cgctggcctc catcgtctgt atcgccacca aatcctacga catccctgtt ctgctggaaa
     2161 ccatcgctac aaagctgagt gaagacacgc tcttcatcgt tgttcagaac ggtttcgccg
     2221 cctatgacat ggtcagcgct gctgtgggcg ctgagcgcgt ggcaatgggc gtcctctacg
     2281 tcggcgcgca gatcatctcg cccgccgtca tcgacattaa gccaggcatc gcacaactct
     2341 ttctgcccgc tcgccatcgc aaatgtctgg gccgcgtggt ggatgcgctc gaaagcggtg
     2401 gcatggaggc cgccctggta gatgatatcg agcggcgtat gtggatgaag cagcttttcc
     2461 tggtgccatt cgccatcgtc aacaccgaga tgcgccagcc tatcggcaaa gtctaccaga
     2521 atgcagccgc gcgcgaacgt tggggcgata tagcgcgcga aatggccgcc gtcgctactg
     2581 cctgcggcat ccccatgccg accgacccgg cagctgccag tctcgccgtt gccgaacgct
     2641 tcgatcccca ggccgactcc tctttcgccc gcgatatctg ggccaatcgc ccccacgaag
     2701 ccgaagcgct cttcggcccg cccctccagc gcgccgccgc gcatggggtg ccttgcccac
     2761 tgttgcaaga ggcatatgag cgctttggaa agcgccaggg cttatccgcc tgctaaaggt
     2821 ttataagtcg aacctggagt acagggtatt cacggtcctc tttttcgctc agagaccggt
     2881 cattcgctcg agttgttcgg gataccgctc cccttgtacc gtgatctttg aaatggcact
     2941 tttgatctcc cgcagatcgt cgggcttcag ttcgacggca agtgcgccga tgttctcatc
     3001 caggcgctcg agcttgcggg agccaggtgt gggaacgatc cacggcttct gagcaagcaa
     3061 ccaggcgagc gcgacggatg gttcctgctc cagcttcaga aaggccgaag tgtttcacct
     3121 tcccttcctc aaacaggtcc ttcaccgccc ccgccacctc ctcgatgggc acgtccgg
//
LOCUS       DASZAK010001330         3824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_138935, whole genome shotgun sequence.
ACCESSION   DASZAK010001330 DASZAK010000000
VERSION     DASZAK010001330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138935"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..891
                     /locus_tag="VGT44_23280"
     CDS             <1..891
                     /locus_tag="VGT44_23280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2583797.1"
                     /translation="GQAFERLGAQVTIIQGPGRILPREDPEVSQTITSVLESEGVQVV
                     TNARFIKAGRNGEKKVITARQGDRLLNFEADEIVLAVGRQPNLESLNLEAAGIAYEEK
                     GITVNDYLQTSASNVLAIGDVVGGYLFTHVAAYQAGVAVRNALVPVAKKKVDYRVVPW
                     CTFTDPEAARIGLTPDQAEKRYKQVRIVKFPWAGIDRAQTEGETVGFIKLVLAGKRDE
                     IVGAHMVGASAGEMLGELSLAMQHNLTLNDVRNTIHAYPTMNIGIQQAAFEGYLESST
                     AASNRKLVRRIANLRDKRIL"
     gene            complement(923..1867)
                     /locus_tag="VGT44_23285"
     CDS             complement(923..1867)
                     /locus_tag="VGT44_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583798.1"
                     /translation="MTLHRDQDYTPLEGSVRSSRHVSRQDILNEKRQRNTDLLPIIES
                     DTTQNGGNARQPNLLRDENRRLRRELEFLQHRLAQQRENEDRFGQEIETIHTGHRLEI
                     EQYQNSLRDLMEELNQKQDALQALDQANQELKYAYHESVERETGKILAEAAQMMEQSP
                     GYTPPVLQGVMTTLEHQLKRTEEHHLAEVMALMRQTQRKNDLLEQELARERESIAQER
                     QKLLVEQTNIREQGKLRQKYVAARLSARFTAFVAVVSTTLVAICVFVQLILVQYFKIP
                     LYWALFSPIFLCFLLALAFARTGSRPDQRKTGKPAKRP"
     gene            complement(2000..2677)
                     /locus_tag="VGT44_23290"
     CDS             complement(2000..2677)
                     /locus_tag="VGT44_23290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583799.1"
                     /translation="MKHTLTPSLLAKFSGICLLFGFMVIIVACGSTAGQVTPGVPAAT
                     VTISFGQVIGSPTSALKGVYCGGWATNATMPYSATGVVNVYGKFTQTGSDGNPTGIGG
                     ATATANISWPDNTSDTISVMTTSDGLAVFTVPMKASAINRIVLIQITFVKGNLSCSIP
                     QAAFFTVIVASPTPTGSVSPSPSGSPNGTNTATPSVTPSITPTGTLTGTPSPVPTLPV
                     PFPSPTV"
     gene            complement(2827..3207)
                     /locus_tag="VGT44_23295"
     CDS             complement(2827..3207)
                     /locus_tag="VGT44_23295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583800.1"
                     /translation="MYKKVFAGLLLFGILTTILAACAIYDEAGTGGPTVHMGNANFVQ
                     TTITISKGQSIDVVDDVAVTHILKNGTWNGGTQVLKSEAGAPTVNVTFNGGDSASIGP
                     FNTAGQFQILCTVHPGMNLTVTVQ"
     gene            complement(3443..3519)
                     /locus_tag="VGT44_23300"
     tRNA            complement(3443..3519)
                     /locus_tag="VGT44_23300"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3483..3485),aa:Glu,seq:ctc)
     gene            complement(3562..>3824)
                     /locus_tag="VGT44_23305"
     CDS             complement(3562..>3824)
                     /locus_tag="VGT44_23305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583801.1"
                     /translation="GATMPYTSVFNEAAHLQGVGRGISQGLVSLISTPSVLLGPPLIG
                     LLFQRTGSITLAFSSILLFSAVAITASFLAGPAVRGETTQST"
BASE COUNT          830 a          996 c         1122 g          876 t
ORIGIN      
        1 gggcaagctt ttgagcgcct cggagcgcag gtcacgatca tccaggggcc tggtcgcatc
       61 ctgcccagag aagacccgga agtgtcgcag accatcacga gcgtcctgga atccgagggc
      121 gtccaggtcg tcaccaacgc gcgtttcatc aaagccgggc gcaacggcga gaagaaggtc
      181 atcactgcca ggcagggtga ccggcttttg aacttcgagg ccgacgagat cgtgctggcg
      241 gtgggaaggc aaccgaacct cgaaagcttg aacctggaag cggcgggcat cgcatacgag
      301 gaaaaaggta tcacggtcaa tgattatctc cagaccagcg cctcgaatgt gctggccatc
      361 ggcgatgtgg tcggtggcta cctcttcacg cacgtggccg cctatcaagc gggggtagcc
      421 gtgcgcaatg ccctagtgcc ggtggcgaaa aagaaggtcg attatcgtgt cgttccctgg
      481 tgtaccttca ccgacccgga ggccgcgcgc atcggcctga cccccgacca ggctgagaag
      541 cgctataaac aggtgcgcat cgtcaaattt ccctgggctg ggattgatcg cgcccagact
      601 gaaggggaga cggtgggttt catcaagctg gtgctggcgg gcaagaggga tgaaattgtc
      661 ggggcgcaca tggtgggggc gagcgcgggc gaaatgcttg gggagctgtc actggccatg
      721 caacacaatc tgacgctcaa cgacgttcga aacaccatcc acgcctaccc aaccatgaat
      781 atcggcatcc agcaggctgc cttcgaaggc tacctggaaa gctcaaccgc cgccagcaac
      841 cgcaaactcg tgcgcagaat cgcaaatttg cgagataagc gcatccttta gtggggcagg
      901 gttgaatagg cgtgtcagag tactacggac gtttggccgg tttccctgtt ttccgctgat
      961 ccggcctgga accggtgcgc gcgaatgcca gggcaagtag aaagcagagg aagatcggcg
     1021 agaaaagcgc ccaatagagc ggtattttga aatattggac caggatcagt tgaacgaaga
     1081 cgcagatggc caccagcgtg gttgacacga cggcgacgaa ggcggtgaat cgagcgctca
     1141 gtcttgctgc gacatacttt tggcgtagct tgccttgctc gcgaatgttc gtttgttcta
     1201 caagcagctt ctggcgttcc tgcgcgatgc tctcccgttc tcgtgccagt tcctgttcga
     1261 ggagatcgtt tttgcgctgt gtctggcgca ttaaggccat tacttcggcc aggtggtgtt
     1321 cttccgtcct tttaagttga tgctccaggg ttgtcatcac gccttgcagg acgggagggg
     1381 tataaccagg ggattgttcc atcatttggg cagcctcggc cagtatcttc ccggtttccc
     1441 tttctaccga ctcatggtac gcatacttga gctcctggtt agcctgatcc aatgcttgca
     1501 gcgcgtcctg cttctggttg agttcctcca tcagatcgcg caggctgttc tgatactgtt
     1561 caatctctag cctatggcca gtatggatgg tctcgatttc ctgcccgaac cggtcttcat
     1621 tctctcgctg ctgcgccagg cggtgttgta agaattccaa ctctcgtcgc agtcgccggt
     1681 tttcatctcg caagagattg ggctggcgag cgttcccgcc attttgcgtc gtatcgctct
     1741 caatgatggg gaggagatcc gtgttgcgct gccgtttctc attcaagata tcctggcggc
     1801 ttacatgacg tgagctacgt accgatcctt ctaacggggt atagtcctgg tcacgatgta
     1861 atgtcatata atcacttcct cgctcgtttt gcgcgcagca atagaaaccg cgtgaatgac
     1921 gcaccgcgtg gatcagatat atatactctc tctgatgtta ttatgcctgt atcgcatcaa
     1981 ttttgcaagt ttttggcttt taaaccgttg gcgaaggaaa ggggactggc agcgtcggga
     2041 ccggtgacgg tgttccggtc aacgttcccg taggcgtgat ggaaggcgtc accgacgggg
     2101 ttgccgtatt cgtgccattg ggcgaaccgg acggagatgg cgataccgaa ccggtgggag
     2161 ttggcgacgc gacaatgacg gtgaaaaaag ctgcctgcgg aattgaacag ctcaggttgc
     2221 ctttaacgaa tgtgatttga atcagcacga tgcgattgat cgcgctggct ttcatcggta
     2281 cggtaaagac ggccagtcca tcgctggtgg tcatgaccga gatggtatcg gatgtgttat
     2341 cgggccagga aatattggct gtcgcagttg ctccgccgat gccggttgga ttgccgtcgc
     2401 tgccggtttg cgtaaacttt ccgtagacgt tgacgacgcc ggtagcggaa tagggcatcg
     2461 tggcattggt agcccatcca ccacagtata cccctttcag cgcggatgta ggagagccga
     2521 tcacctgccc aaaactgatc gtgaccgtcg ctgcaggcac gcctggtgtt acctggcctg
     2581 ctgtgctgcc acaggcgacg ataatcacca taaagccaaa tagcaagcat ataccagaaa
     2641 attttgccag gagagacgga gtcaatgtat gtttcatgcg ttatcgccgt acatcatatt
     2701 gttgcgtttg tccgtgtaaa ccttccgaat caatactggt cagttgtcat tagtggataa
     2761 gatagcatgg actggaagga ttgtcgaatg aaccctgtct atctgccata aaagtactag
     2821 aacatgtcat tgcaccgtca cggtcagatt catgcctggg tggacagtgc agagaatttg
     2881 aaactggccg gcagtattga atggcccgat ggacgcggaa tcgccaccgt taaaggtcac
     2941 attgacggtc ggcgcgcccg cttctgattt gaggacctgc gtcccaccat tccaggtgcc
     3001 gtttttgaga atatgcgtca ccgctacatc atcgaccacg tcaatcgatt gacccttgct
     3061 gatggtgatg gtcgtctgca caaaattggc gttgcccatg tggacggttg gcccacctgt
     3121 gccggcttca tcgtaaatcg cgcaggccgc gagtatggtt gtcaaaatac cgaaaagaag
     3181 tagacccgca aagacctttt tatacataga acgttccttt caaattgggg aaaatacccc
     3241 ttgattgctg ccgagacggt acgcgttgat tatgtgcata cccttactac ttatcatagc
     3301 aaatgggggc gaaagctacc agggggctga aatgtaccgg acccaattgt ttgtaatctt
     3361 ctgatgaatg ataggccgaa agccaggagt ggctgtctca agcaactttt cctgtggcat
     3421 gaggtggttc taggtgcttc gctggtagcg catacgggag tcgaacccgt gatctccgcc
     3481 ttgagagggc ggcgtcctgg accactagac gaatgcgcct cgttgactgt ggcaatatca
     3541 taccgctcac cagggctttt gtcaagtgga ctgcgtcgtt tcgccccgca cggctggccc
     3601 ggccagaaac gacgcggtga tggcaacggc ggagaagagc aggatggagc tgaaggcgag
     3661 cgtgatgctg ccggtgcgct ggaacagcaa gccgatcagc ggcggcccca acagcacgga
     3721 gggcgtcgag atcaggctga ccagcccctg agaaatgccc ctcccaacgc cctgcaaatg
     3781 tgctgcctcg ttgaagaccg aggtataggg catcgtcgcg ccga
//
LOCUS       DASZAK010001331         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_390147, whole genome shotgun sequence.
ACCESSION   DASZAK010001331 DASZAK010000000
VERSION     DASZAK010001331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390147"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VGT44_23310"
     CDS             <1..308
                     /locus_tag="VGT44_23310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979455.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV2583802.1"
                     /translation="LIVHKDSAYVRGRDLCEKMQELIPRQMFEVAVQAAIGSHVIART
                     SVKALRKNVTAKCYGGDITRKRKLLEKQKEGKKRMKQIGTVEIPQEAFLAVLHVGKK"
     gene            353..1144
                     /gene="lepB"
                     /locus_tag="VGT44_23315"
     CDS             353..1144
                     /gene="lepB"
                     /locus_tag="VGT44_23315"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV2583803.1"
                     /translation="MHFDFEAILTLATAFTGIVWLVDALWFAKRRRAVMPAEAAEAPA
                     KARDPILVDYSKSFFPVILLVLLLRSFLAEPFHIPSSSMVPTLLVGDFILVNKYDYGI
                     RLPVLHTKIINVGEPKRGDVVVFRYPGDTSVDYIKRIVGLPGDHIVYRNKTLYINGVM
                     MQKDALGAYSGPDADGAELNREHLGDVVHQTLTIPGQYSREGEWTVPEGEYFAMGDNR
                     DNSSDSRYWGFVPEQNLVGKAFFIWMNIDAFKDSSLWHRVGNTIH"
     gene            1185..1565
                     /locus_tag="VGT44_23320"
     CDS             1185..1565
                     /locus_tag="VGT44_23320"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HEV2583804.1"
                     /translation="MTMRSRQGGMTMISWILLIGLIGFVGIFAFKLIPVYLDYFSLNK
                     AMTTVAKNLQPGETPQQIQFAISSAFDINSVNVMKYTDVSIKVDPDTKAVTLGVDYDD
                     RTNFLANIDLVVHFDKTYSAAGAH"
     gene            1562..>2058
                     /gene="rnc"
                     /locus_tag="VGT44_23325"
     CDS             1562..>2058
                     /gene="rnc"
                     /locus_tag="VGT44_23325"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV2583805.1"
                     /translation="MTGSRTALLRALGRGFSDETLLDLALTHRSVGGRNNERLEFLGD
                     ALINACVADALYQRHTELEEGDLSRLRASLVNQEALADVARELDLGSYLSLGPGELKS
                     GGHRRASILADALEALIGAVFLDAGFEAAHAVVSRLLESRLAAPLSTEALKDSKTRLQ
                     EVLQAR"
BASE COUNT          424 a          603 c          628 g          403 t
ORIGIN      
        1 cgctcatcgt gcacaaggat agcgcctatg tacgcggacg cgacctgtgc gagaagatgc
       61 aggaactcat tccccggcag atgttcgagg tggcggtgca ggcggccatc ggttcccacg
      121 tcatcgcgcg cacttccgtg aaggccctgc gcaagaacgt gacggcgaag tgctacggtg
      181 gcgacatcac ccgcaagcgc aaactgcttg agaagcagaa ggaaggcaag aagcgtatga
      241 agcagatcgg tacggtggag atcccccagg aggccttcct ggcggtgctc cacgtcggca
      301 agaagtgacg cactttcacg ctatcgtcaa aatccaggcg taggaaacaa caatgcattt
      361 cgatttcgaa gccatcctga ccctcgccac cgccttcacc ggcatcgtat ggctggtgga
      421 cgctctctgg ttcgcgaaac gccggcgggc ggtgatgccg gccgaggcgg cagaagcgcc
      481 ggccaaggcg cgtgacccca tcctggtgga ctattccaag tccttcttcc cggtgatcct
      541 gctggtgctc ctgctgcgct ctttcctggc ggaacccttc catataccgt cttcctccat
      601 ggtaccgacg ctgctggtgg gggatttcat cctggtgaac aaatacgact acggcatccg
      661 cctgccggtg ctgcacacca agatcatcaa tgtgggcgag cccaagcgcg gggatgtggt
      721 ggtgttccgt tacccaggcg acacaagtgt ggattacatc aagcgcatcg tcggacttcc
      781 cggcgaccat attgtctacc ggaataaaac gttgtatatc aatggggtca tgatgcagaa
      841 ggacgcgctc ggcgcctatt ccgggcccga tgcggacggc gctgaattga accgcgagca
      901 cttaggtgac gtcgtgcatc agacgctcac catcccgggt cagtacagcc gggaagggga
      961 atggacggtg cccgagggcg agtacttcgc catgggggat aaccgcgaca acagcagcga
     1021 cagccgctac tggggtttcg tgccggagca gaacctggtg ggcaaggcct tcttcatctg
     1081 gatgaacatc gatgctttca aggacagcag tctttggcat cgcgtcggca acacgattca
     1141 ttgaacgtct ggcagcggcc atggccgcac ctgggagaac cacgatgact atgcgttcgc
     1201 gtcaaggcgg catgaccatg atcagctgga tattgctcat cggcttgatc ggcttcgtgg
     1261 gtatcttcgc gttcaagctg atccccgtat acctggatta tttcagcctg aataaggcga
     1321 tgacgaccgt ggcgaagaac ctgcagccag gcgagacacc acaacaaatc cagttcgcca
     1381 tttccagcgc cttcgacatc aatagcgtca atgttatgaa gtacacggac gtgtcgatca
     1441 aggtcgatcc ggataccaag gcggtgaccc tgggtgtcga ttatgatgat cgcaccaatt
     1501 tcctggccaa catcgatctc gtggtccact tcgacaagac ttactcggcg gccggcgcgc
     1561 attgaccggt agccgtacag cgctgctgcg ggcactgggc aggggattca gcgacgagac
     1621 tctgcttgac ctggctctca cccaccgcag cgtcggcggc aggaataatg aacgcctgga
     1681 gttccttgga gatgccctga ttaacgcctg cgtggccgat gctctctacc agcgccatac
     1741 agagttggag gagggtgatc tgagccgtct ccgggcgagc ctggtgaacc aggaagcgct
     1801 tgcggatgtg gcacgggaac tcgatctcgg cagctatctc agtctcggtc cgggagagct
     1861 caagagcggc gggcaccgcc gtgcctccat cctggcggac gcactggaag ccctcatcgg
     1921 tgccgtgttc ctggatgcgg gtttcgaggc cgcccatgcg gtcgtgtcga ggctcctgga
     1981 gtctcgcctc gcagcaccgc tctccacgga agccctgaag gattccaaga cgcgcctgca
     2041 ggaagtcctg caggcgcg
//
LOCUS       DASZAK010001332         4399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_106323, whole genome shotgun sequence.
ACCESSION   DASZAK010001332 DASZAK010000000
VERSION     DASZAK010001332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106323"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1059)
                     /locus_tag="VGT44_23330"
     CDS             complement(94..1059)
                     /locus_tag="VGT44_23330"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEV2583806.1"
                     /translation="MLNDPILINDWHVVAYAPDLQEGKPVAARLLEEDLVLWRAGETI
                     HAWRDLCLHRGTRLSLGKVENDCLICPYHGWTYNEEGQCIRFPAHPHQKPPATAHTKV
                     YRAQERYDWIWVTLGDPARDIPEFPQWDDHAFHKVHCGPYTFHASGPRAIENFLDVTH
                     FPFVHTGLLGDPNYPEVNDYEVETTPGGITARNITVWQPDPDGTGQGASVTYIYNVLR
                     PLTACFTKSSSGPRFAMYFTITPVAERESIAWTYVARDYDAIPDDEVRQFEDMITLQD
                     IPIVESQRPELLPLDLQAELHLRSDRTAIAYRRWLREMGLTFGTA"
     gene            1345..2154
                     /locus_tag="VGT44_23335"
     CDS             1345..2154
                     /locus_tag="VGT44_23335"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV2583807.1"
                     /translation="MERRTLGNAGLSVPVVGMGTWKTFDVSGSTAINNARAVVDAALQ
                     GGADLFDSSPMYGQAEYVLGEALRGRRDQVLVATKIWTQTRREGQEQARRALQYFGGC
                     IDLYQIHNLVNWREHLTMLERLRDAGQVRAIGATHYESSAFGDLSQVMQTGRITAIQI
                     PYNPFEREVEQVILPLAADLGLGVVVMRPFAQRALMQRPPTEKELEPLKPFGVTTWAQ
                     ALLKWILSDPRCHVAIPATTSPARMAENAAAGDPPWFGEQERAYVAGLATR"
     gene            2165..3190
                     /locus_tag="VGT44_23340"
     CDS             2165..3190
                     /locus_tag="VGT44_23340"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEV2583808.1"
                     /translation="MREQPKISEERLRDCLQGQYGLIPATLEFLPRGKDYNAGVYRVV
                     SEQGIPYLLKIKSGALYEPGCLVPGYLSNQGITSVVAPIPAKNNALWTRLENWTVIVY
                     PFIDGDTSLRGMTDEQWKETGTIFRRIHQVMLPPFGFESLRKESFDPTGYARWIRDFE
                     TQHLHSRHGGSASKRALHADWMAHQSTIHTGVTSLEKLAEALHSQTFPYGICHADLHP
                     ANLIRDRAGHVFVIDWDDVMLAPKERDFIFIRELQAGAFFQGYGQTAIDWVALTYYQW
                     ERVVTDLIECAKDVCFRDDLGEETRAEIAELFHEGLVRKGGNVDAAYAAAAHLPSKLA
                     ALLASLL"
     gene            complement(3182..>4399)
                     /locus_tag="VGT44_23345"
     CDS             complement(3182..>4399)
                     /locus_tag="VGT44_23345"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HEV2583809.1"
                     /translation="QAYEARITKTYDEGLGVLFGEEPAPYIRQCANKCVFCFIQGLPE
                     RFSVQPGLPHGMRSTLYIKDDDYRYSFMFGNFITLTNLKELDWQRLDEQKLTPLYVSV
                     HTTDPDLRRKMVDGPRAGDIIDHIKRLGDMGITCHTQLVLCPPINDGPHLERSIRDLA
                     ELRPIVESISVVPVGLTKYNNMIKTGDLPPLRHYTRAEAETVIEQTEAYQRQFAAADP
                     DGSPFVYLSDEWYYLTERDFPPAQHYGTFAQIENGVGMTRYLVDQWASGKRRLPARIS
                     RPRRVTLVTSTMARPIIEQFATDLRAIAGLEVQVLPIVNRFFGPEVTVAGLLCGQDVL
                     AALQANHNLGDLILLPRVMLDNEGTRFLDDMTVEEFKQQLPVRAEFARNAPEMIAAIN
                     ALATQKPTLPVLQ"
BASE COUNT          859 a         1143 c         1480 g          917 t
ORIGIN      
        1 atggtccgag atgcttcgct gcgctcagca tgacagggga ggcaacttcg gtgaacccct
       61 ttgagggtgt atcctcgcgg tcgccatgct acgttacgcc gtcccaaacg tcaatcccat
      121 ctcgcgcagc cagcgccggt aggcgatcgc ggttcggtcg gagcgcaggt ggagttcggc
      181 ttgcaggtcg agggggagga gttcggggcg ctgcgattcg acgatgggga tatcttgtag
      241 ggtaatcatg tcctcgaatt gacgcacctc gtcgtcgggg atggcgtcgt agtcgcgggc
      301 gacgtaggtc caggcgatgg attcgcgttc ggcgacgggc gtgatggtga aatacatggc
      361 gaaacggggg ccgctggatg atttggtaaa gcaggcggtc agggggcgca gcacgttgta
      421 gatataggtg acactggccc cctggccggt gccatcgggg tcgggctgcc agacggtgat
      481 gtttctcgct gtgatgccac ctggtgtcgt ctcaacctcg taatcgttca cttcgggata
      541 gttcgggtcg cccagcaggc cggtatgcac gaaggggaag tgcgtcacgt cgaggaagtt
      601 ttcgatggca cgcggaccgc tggcgtgaaa ggtgtatggg ccgcagtgga ctttgtggaa
      661 ggcgtggtcg tcccattgag ggaactccgg gatatcgcgc gccggatcgc ccagcgtgac
      721 ccagatccag tcgtagcgtt cttgagcgcg gtagactttt gtgtgagccg tcgcaggcgg
      781 cttctggtgg gggtgcgctg gaaagcggat gcactgtcct tcttcgttgt aggtccagcc
      841 gtgataggga cagatcaggc aatcattctc gaccttgccc aacgagaggc gcgttccacg
      901 gtgcaggcac aggtcgcgcc atgcgtggat cgtctcgccg gcgcgccaca ggaccaggtc
      961 ttcttcgagc aaacgcgcgg ccaccggctt gccctcttgc aggtcgggcg cgtaggccac
     1021 cacgtgccag tcgttgatga ggatggggtc gttgagcatg ggttagtccc tttctagcga
     1081 atggatcggt gtaccctcgc ggaatgaaat ttcaccaccg ggaatgtcac cctgagcgca
     1141 gcgaagggtc tgatgcgcca gggaccgaga tccttcgctg cgctcaggat gacaagatca
     1201 gttttggccg ggaaacttta taattcacat gcccagtata acatggcgta acggcggcat
     1261 ggaattgacg tggaaatggg gtataataag tggagttgaa tatgcgtcaa taggcttgaa
     1321 aagctgtatg aagaggagct acgaatggaa cgccgcactc tgggcaatgc cggcctgagc
     1381 gtgcctgtcg ttggcatggg aacctggaag accttcgatg tgagcgggag caccgccatc
     1441 aacaatgcac gcgcggtcgt cgatgccgcg ctgcaaggcg gggcggatct ctttgactcg
     1501 tcgccgatgt atgggcaggc ggagtatgtg ctgggcgagg cgctgcgcgg caggcgtgac
     1561 caggtcctgg tcgcgacgaa gatctggacg cagacgagac gcgagggaca ggagcaggcc
     1621 aggcgcgcac tgcaatactt cggcgggtgt atcgatctct accagattca taacctggtc
     1681 aactggcgcg agcatctcac catgctggaa cgcttgcgcg acgccggtca ggtgcgcgcc
     1741 atcggggcca cacactacga gagttcggcc ttcggcgatt tgagccaggt gatgcagacg
     1801 gggcgcatca ccgccataca gataccctac aatccgtttg agcgtgaagt ggagcaggtg
     1861 attttgccgc tcgccgccga tctcggcctg ggcgtggtcg tgatgcggcc gttcgcccag
     1921 cgagcgttga tgcagcgccc gcctacagaa aaagaattgg agccgctcaa gccctttggc
     1981 gtgactacct gggctcaggc tctgctcaaa tggatactga gcgatcctcg ctgccacgtc
     2041 gctatccccg ccactaccag cccggcgcgc atggccgaga acgctgccgc cggagacccg
     2101 ccctggtttg gcgagcagga acgcgcatat gtggcgggac tggcgacgag ataaaagcat
     2161 agttatgcgt gaacaaccaa agatttcaga ggaacgcctg cgggactgct tacagggaca
     2221 gtacggcctg atccctgcca cgcttgagtt tcttccacgg gggaaggatt acaatgccgg
     2281 ggtataccgc gtggtgagcg agcagggaat tccctatttg ctcaagatca agtctggagc
     2341 gctttacgaa ccaggctgtc ttgttcctgg ctatctcagc aatcagggga tcacatcggt
     2401 tgtggctccc attcccgcga agaacaatgc cttgtggacc cggctagaga attggacggt
     2461 gatcgtgtac ccctttatcg atggggatac gagtttgagg gggatgacgg atgagcagtg
     2521 gaaggagaca ggaaccatct tcagacggat tcaccaggtt atgctgcctc ctttcggctt
     2581 tgagtcattg cgaaaggaga gctttgatcc gacgggctat gcccggtgga tacgcgactt
     2641 tgagacgcaa cacctccatt cgcggcacgg tggaagcgcc tctaagcgcg ctcttcacgc
     2701 cgactggatg gcgcatcagt ctacgataca tacgggagtg acttccctgg aaaagctggc
     2761 agaggcactt cattcgcaga cattcccata cggcatctgt cacgccgatc tgcatccggc
     2821 caatctgata cgtgatcgcg ccggtcacgt gtttgtaatc gattgggatg atgtgatgct
     2881 ggcacccaaa gagcgcgatt ttatctttat cagggaacta caggccgggg cgttttttca
     2941 ggggtacgga cagacggcga ttgattgggt tgccctgacg tactatcagt gggagcgggt
     3001 tgtgacagat ctgatcgagt gcgccaaaga cgtgtgtttc agggacgatt tgggagagga
     3061 aaccagggct gagatcgccg agttgtttca tgagggcctg gtgaggaaag ggggcaatgt
     3121 cgatgctgct tatgcagcag cggcccatct tccctcgaag ctcgccgctc tattggcatc
     3181 tctactgtaa caccggcagt gtcggctttt gcgtggccag agcgttgatg gcggcgatca
     3241 tttctggggc gttgcgtgcg aactcggcgc gtaccgggag ttgctgcttg aattcttcga
     3301 cggtcatgtc gtcgaggaag cgggtgcctt cgttatccag cataacgcgg gggagcagaa
     3361 tcagatcgcc caggttgtgg ttagcttgca gggcggccag cacgtcctgg ccgcagagca
     3421 ggccggcaac ggtgacttcg gggccgaaga agcggttgac gatgggcagg acctgcacct
     3481 ccaacccggc gatggcgcgc aggtcggtgg cgaactgttc gataatgggg cgcgccatcg
     3541 tgctggtgac gagcgtgacg cggcgtggtc gggatatgcg ggcgggcagc ctgcgtttgc
     3601 cgctggccca ttgatcgacc aggtagcgtg tcatgccgac gccgttctcg atctgcgcga
     3661 aggtaccgta gtgctgcgcg ggcgggaagt cgcgttccgt caggtagtac cattcatcgg
     3721 agaggtaaac gaagggggag ccgtcggggt cggcagccgc gaattgtcgc tggtatgcct
     3781 cggtctgctc gatgacggtc tcagcctcgg cgcgggtata gtggcgcagg ggaggcaggt
     3841 cgccggtttt aatcatattg ttgtacttcg tcagcccgac ggggacgacc gagatcgatt
     3901 cgacgatggg gcgcagttcg gccaggtcgc ggatgctgcg ctcaaggtga gggccatcgt
     3961 tgatgggcgg gcaaagcacc agctgcgtgt gacaggtgat gcccatatca cccaggcgct
     4021 tgatatggtc aatgatatca cccgcgcgtg gaccatcgac cattttgcgg cgcaggtcgg
     4081 gatcggtagt atgcaccgag acgtagaggg gcgtcagctt ctgctcgtcg aggcgctgcc
     4141 agtcaagctc tttcaggttg gtcagcgtga tgaagttgcc gaacatgaac gaatagcggt
     4201 agtcgtcatc cttgatgtag agcgtcgagc gcatcccatg cggcaggccc ggctgaacgg
     4261 agaagcgttc gggcaggccc tggataaaac agaagacgca tttattggcg cactggcgaa
     4321 tgtagggtgc gggttcctcg ccgaagagta cgcccaggcc ctcatcgtag gttttggtaa
     4381 tacgcgcttc ataagcttg
//
LOCUS       DASZAK010001333         2553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_532270, whole genome shotgun sequence.
ACCESSION   DASZAK010001333 DASZAK010000000
VERSION     DASZAK010001333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532270"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VGT44_23350"
     CDS             complement(<1..630)
                     /locus_tag="VGT44_23350"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV2583810.1"
                     /translation="MPVFNRHQQRNTVEQLVEEYTTFRINRRQFMQRAMAVGLSTGAA
                     VSLLEACGSPSPTVGSGTPTTVKSIDSLVVISGTELDNYKAVNAAFTAKTGITVNVES
                     TRDLPTVLNTRIRGNNPPDIAAPPLGILQTLAGQGKLLRLDSFLDMSTINSNYSSTWI
                     NLVSVNGGVYGVPFLANTKGCIWYSPKQFQANGWTTPTTWQDMITLSNKI"
     gene            complement(1007..2326)
                     /locus_tag="VGT44_23355"
     CDS             complement(1007..2326)
                     /locus_tag="VGT44_23355"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2583811.1"
                     /translation="MSNQPPCVAWAEKLALRHEDLSPADRDALDVHIQSCSACEATQA
                     DYRFLDARLRALPLPTMQPLPRLAAALAKREEKEEHRADNAEEQTQRRPASRERELPR
                     PTPQHFSVWRVRKLLPAAFVALLILSLIALFSSRFVNTTAGYPLGATLYTYQGHGDFV
                     DAVAWSPDGRYIASGSWDHTVRVWDAKSRTLLLTYSHSDIVDAVAWSPDGRYIASGSW
                     DNTIQVFDVSTGNRIMLYQGHSDVVSSLAWSPDGSYIASGSWDHTVRVWDVYTGGLVF
                     KTRFNDFVDSVAWSPNGRSLVVGGRDETVYVLDARTGAQKFSLSGESSVVNTVAWSPD
                     GRYIAAGYRDGVVQIWSAAGAFLSAYKVDSNEVRSLAWSPDSNYIASGGWDHTVRVWS
                     SKTGVPLVIYRGHTDNVDAVAWSPGGHLVASASWDTTVQVWQATLGG"
     gene            complement(2331..>2553)
                     /locus_tag="VGT44_23360"
     CDS             complement(2331..>2553)
                     /locus_tag="VGT44_23360"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEV2583812.1"
                     /translation="TELLKLALARVSGTYRPCLILYVIEELPQRQIADLLKIKESSVS
                     KYVSRGKEELRQIYHRLMKDQDAPARGRR"
BASE COUNT          552 a          705 c          751 g          545 t
ORIGIN      
        1 gatcttgttc gagagcgtga tcatatcctg ccaggtcgtg ggagtggtcc agccattggc
       61 ctggaactgt ttggggctat accagataca gcccttggtg ttggccagga agggtacacc
      121 gtacacaccg ccattgaccg agaccaggtt gatccaggtc gatgagtagt tcgaattgat
      181 cgtgctcata tcgaggaagc tgtccaggcg cagcagtttc ccctgacctg ccagggtttg
      241 gagaataccc agaggggggg cggcgatgtc gggcggattg ttgcccctga tgcgcgtatt
      301 cagaacggtc ggcaggtcgc gcgtagactc gacgttgacg gtgatgcctg tcttcgctgt
      361 gaatgccgcg ttgacagctt tgtagttatc cagctccgta ccgctaatca ccaccagcga
      421 gtcaatggat ttgaccgtgg tgggggtgcc agatccaacc gtggggctgg ggctgccaca
      481 ggcctctagc aacgacacgg ctgctccggt agacaggccg acagccatag cgcgctgcat
      541 aaactgcctg cgattgatcc tgaatgtcgt gtactcttct actaattgct ccactgtgtt
      601 gcgctgctga tgacgattaa atacaggcat tgttccctcc tttgcagggt gtaggcgact
      661 aaaaggacaa ggtggtcaag gaatgccgtg tcaggctgag atcgctgtac cgggagagcg
      721 agcgtgagtc aggaataagc aatgaatcgt tgcgacacgt cgctcgacag agacccaacc
      781 ttatacgata gtgtaaagta ctacggtact aagaaggaac gtcttttggg gggctaacag
      841 ggggcgtttt gctaagccca tccatagccc catagtaggg acgcgatgat gagttttcac
      901 gttaaattgt taatctccaa catcgtatct acttatacta cgtgggcaca gggcaataga
      961 ctcacgctca agctcacctc atataaggcg ggtaggggcg acagctttac ccgccaagag
     1021 tggcttgcca tacttgaacg gtggtatccc aactggcgga agcgacgagg tgaccgccgg
     1081 gcgaccaggc caccgcatcg acgttgtcgg tatgtccgcg atagatgacg agagggactc
     1141 ccgttttgct actccagacg cgcacagtat gatcccagcc tccggaggcg atatagttgc
     1201 tatctggcga ccacgccagc gatctcacct cgttgctatc tactttgtag gcagagagaa
     1261 aagctcccgc cgcactccat atttgtacga caccatctct gtatccggca gcgatgtagc
     1321 ggccatcggg cgaccacgcc accgtattaa caacgctcga ttctcctgag aggctgaatt
     1381 tctgcgctcc cgtcctggca tccagcacat ataccgtctc gtctcgtcca ccgacaacaa
     1441 gcgagcgacc attgggcgac caggcgacag aatcgacgaa atcgttaaat ctggttttga
     1501 agacaagccc gcccgtgtag acatcccaga cgcgcacggt atgatcccaa ctgccggagg
     1561 cgatatagct gccatcgggc gaccacgcca gcgaactgac gacatcgcta tggccctggt
     1621 agagcatgat gcggttgccc gtgctgacat caaatacctg gatggtatta tcccaactgc
     1681 cggaagcgat atagcggcca tcgggcgacc acgccaccgc gtcgacaata tcgctgtggg
     1741 agtaggtcag gagaagcgtt cttgacttcg catcccagac gcgcacggta tgatcccaac
     1801 tgccggaggc gatgtagcgg ccatcgggcg accacgccac cgcgtcaaca aagtcaccat
     1861 ggccttgata ggtgtaaagg gttgcaccca aaggatagcc ggctgtcgta ttgacgaagc
     1921 gcgagctaaa gagagcgatc agggagagta tcagcagggc aacgaaggct gctggaagca
     1981 gcttcctcac gcgccacacg gagaaatgtt gaggcgtcgg gcgtggtagc tcccgctcgc
     2041 gacttgcagg acggcgctgc gtttgctctt cggcattgtc cgcgcggtgc tcttcctttt
     2101 cctctcgctt tgccagcgca gcggccaggc gcggcagggg ctgcatggtt ggcagaggca
     2161 gcgcgcgcag gcgggcatcg aggaagcgat agtcggcctg ggtcgcctcg cacgccgaac
     2221 agctctggat atgaacatca agcgcgtcgc gatcggctgg cgaaaggtcc tcgtgcctga
     2281 gcgcgagttt ctcggcccag gccacgcaag gaggctggtt gctcatgcag ctatctcctc
     2341 cctctcgccg gagcgtcctg gtccttcatg agccggtgat agatttgtcg taactcttct
     2401 tttccccgac tgacatactt gctcacgctt gactccttga tttttagcag atcagcgatc
     2461 tggcgttgcg gcagttcttc gatgacatat aaaatcaggc atgggcggta ggtcccagag
     2521 acgcgcgcga gggcaagttt cagtaattcc gtt
//
LOCUS       DASZAK010001334         2467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_532276, whole genome shotgun sequence.
ACCESSION   DASZAK010001334 DASZAK010000000
VERSION     DASZAK010001334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2467
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532276"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VGT44_23365"
     CDS             <1..405
                     /locus_tag="VGT44_23365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV2583813.1"
                     /translation="RELLRRPDVTYTQVARLSQSQPQHSAQTSTGDQTVLPLLEETTA
                     EEVELQVKYENYVRKQEQQVHRARRLEERLIPETLDYNVVQHMRTQARQKLLHMRPRT
                     VGQASRVEGVTPADIAILLIYMEKERIALRNI"
     gene            561..1244
                     /locus_tag="VGT44_23370"
     CDS             561..1244
                     /locus_tag="VGT44_23370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583814.1"
                     /translation="MYILVIDDDQFANSLIHFVLSKEGYEVETVDSPRGVIQMIQKRE
                     PDLLIVDVMMPYLNGFEFARKLHDDGYDIPFIFITAQDGIDKKLEGFHIGADDYICKP
                     YNHQELVSRVQAVMRRIKKSGKGGNGTIRCGQVELLPADLQVMIPGHSPVLLTPTEMH
                     VLRVLMSSQGQVVNRDHLLSSVWNENENNSNIVDVYIRRLRMKLEEDADKPQHIMSIR
                     GIGYKFVGK"
     gene            1254..2435
                     /locus_tag="VGT44_23375"
     CDS             1254..2435
                     /locus_tag="VGT44_23375"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583815.1"
                     /translation="MPDRAQEPHQSSLSSTEGEEPVIITALDDAISDMKQHEYLWPLF
                     NNSSNSIIVLNTQYQVIDFNPTTVEKLGWQRETTIGKPCSEVLCCKNLNGLVLCGTSS
                     CPLTRVLKQNKPIPNEELICGALPGHIAEYAVGVTPIPSPIGTVVVFSARDVTSSKVA
                     NSVRANFVSMVSHELRTPLNSVHGFIDLLMQGHMGELTTEQKTYLGYAQEGVQQLMTI
                     VEDILLLTRSDTGQFDMRQEAVNLLTLAKQALRGLQPQAQKASVVLNRDISSPAPMLY
                     ADPQRIMQVLNNLLTNAIKFTPPGGSVTLHARPYDERFVLISVQDTGFGIASEDRPHV
                     FERFYQANHHLQSKMGGYGLGLTIARLIVEQHGGQINFDSHVNQGTTFFFTIPLYTPR
                     A"
BASE COUNT          585 a          725 c          660 g          497 t
ORIGIN      
        1 cgcgaactac tgcgccgacc ggacgtgacc tatactcagg tggctcgcct gtcacagtca
       61 caacctcagc acagcgctca gacctcaacc ggcgatcaaa ccgttttacc tttgctggaa
      121 gagacgactg cggaagaggt cgagctgcag gtaaaatacg aaaactacgt gcgcaagcag
      181 gaacagcaag tacaccgggc gcgccgcctg gaagaacgac tcatcccgga aacgctggac
      241 tacaatgtcg tgcagcacat gcggactcag gcgcgccaga aactgctgca catgcgcccg
      301 cgcaccgtcg ggcaggcttc gcgcgtcgaa ggcgtcacac cggcggatat cgccatcctg
      361 ctgatctaca tggagaaaga gaggatcgcc ctccgaaaca tttaggcgaa cttgacatcg
      421 cgattgcgca aagtgtaaca taaaatgcag tagaactaca attaccttta tgtatcgaat
      481 tgtcgtcctg cttacccgtg tgatcccccc cacgcgggtc ggtagggcca ggaaatcgcg
      541 cgctgaaaag ggggaacagt atgtacattc ttgtcattga cgacgaccag tttgcaaact
      601 cactcatcca ctttgtcttg agcaaagagg gatacgaagt cgaaaccgtt gatagtcctc
      661 gcggcgttat acagatgatc cagaagcgcg agcctgacct tctgatcgtg gatgtcatga
      721 tgccctacct caacggcttt gaattcgcca ggaaactgca cgatgatggc tatgatatcc
      781 catttatttt tatcacagcg caggatggca tagacaagaa gttggaaggc ttccatatcg
      841 gcgcggacga ttatatctgc aaaccttaca accaccagga actggtgtcg cgggttcagg
      901 ccgtgatgcg ccgcatcaag aagagcggga aaggtggcaa tggcaccatt cgctgtgggc
      961 aggtcgaatt gctgccggca gacctgcagg tcatgatccc cggccattca cccgtcctcc
     1021 tgacacccac tgagatgcac gtgctgcgcg tcctgatgtc ctcacagggc caggtggtca
     1081 atcgcgatca cctgctttca tcggtctgga acgaaaacga gaacaacagc aacattgtcg
     1141 atgtctatat tcgccgcctg cgcatgaagc tggaagaaga tgcagacaag ccacagcata
     1201 ttatgtcgat ccgtggtatc gggtacaaat ttgtagggaa atagaggaac atcatgcctg
     1261 atcgcgcgca ggagccgcac cagtcctctc tgtcatcgac ggagggagaa gaaccggtaa
     1321 tcatcactgc cctcgatgat gctatttcag atatgaaaca gcacgaatac ctgtggccgt
     1381 tgttcaacaa ctcatccaac agcattatcg tgctcaatac gcagtatcag gtgatcgatt
     1441 tcaatccgac aacggtcgag aagttaggct ggcagcgcga aacaaccatc gggaaaccat
     1501 gcagtgaggt cctgtgctgc aagaacctga atgggctggt cttgtgcggc acttcaagct
     1561 gcccgctcac gcgcgtcctc aagcagaata agcctattcc caacgaagag ttgatctgcg
     1621 gcgcgctccc cggccatatt gctgaatatg cagtcggggt cacgcccata ccgtcgccaa
     1681 tcggcacagt ggtcgttttc agcgcgcgcg atgtcacctc ctcaaaggtg gccaacagtg
     1741 tgcgcgccaa tttcgtctcg atggtctcgc atgagctgcg cacgcctttg aactctgtgc
     1801 atggcttcat cgacctgctg atgcagggac acatgggcga gctgacgacc gagcaaaaaa
     1861 cctatctcgg ctatgcccag gagggtgtgc agcagttgat gacgattgtg gaagatatcc
     1921 tgctcctgac gcgctccgac acaggccagt tcgacatgcg ccaggaggcg gtcaacctgt
     1981 tgacgctggc gaaacaggcc ctgcgcggct tgcagcccca ggcccagaag gcttccgtcg
     2041 tgctgaatcg cgatatctcg tcgcccgcgc ctatgcttta cgccgatccc cagcgcatca
     2101 tgcaggttct caacaacctg ctgaccaacg ctatcaaatt cacacccccc ggcggctccg
     2161 ttacgctgca cgcccgtcca tacgacgaac gtttcgtcct catctccgtg caagataccg
     2221 gcttcggtat cgcgtccgaa gatcgccccc acgtcttcga gcgcttctac caggccaacc
     2281 accacctgca atccaaaatg ggcggctacg gcctcggcct gaccatcgcc cgcctgatcg
     2341 ttgagcagca cggtggccag atcaacttcg actcgcacgt caaccagggc acgaccttct
     2401 tcttcaccat tccgctgtat actcctcgtg cgtagggaca gggggaggag gcgtggagcg
     2461 gcggggg
//
LOCUS       DASZAK010001335         4101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_68992, whole genome shotgun sequence.
ACCESSION   DASZAK010001335 DASZAK010000000
VERSION     DASZAK010001335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68992"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..625)
                     /locus_tag="VGT44_23380"
     CDS             complement(92..625)
                     /locus_tag="VGT44_23380"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV2583816.1"
                     /translation="MSSAPESIRLEDWIRDIPDFPQKGILFKDITPLLQDAKAFHAAM
                     DRLAAHYAGSGIQVVVGVESRGFIFGAALAYLLNCGFVPVRKFGKLPHQTISVEYSLE
                     YGTNVVEMHADAIRPGQRVLIVDDLLATGGTTSAAMELVEQLGGHIAGIAFLVELAFL
                     KGREQLGGHDVFALIKY"
     gene            complement(762..1223)
                     /gene="mscL"
                     /locus_tag="VGT44_23385"
     CDS             complement(762..1223)
                     /gene="mscL"
                     /locus_tag="VGT44_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV2583817.1"
                     /translation="MASERIRRLERSATGTLGGFRTFILRGNVVDLAVGIVIGAAFSG
                     VVTSLVSNVITPLIPAPGGSLSTWFITIAWTHKPLNIGTFINAIISFLIIALVIYYFV
                     VLPVNTLMARFKPKEVPAPATMRDCPYCFSSIPIQASRCPNCTSQVQPLTP"
     gene            complement(1322..3814)
                     /locus_tag="VGT44_23390"
     CDS             complement(1322..3814)
                     /locus_tag="VGT44_23390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV2583818.1"
                     /translation="MHEKSIQTLEFPKILERVAKEAAFSASKELVRALEPTPNIDEAR
                     RRLAYTSEAYKLIDLRADAGVQGARDIRAHLSRAAREGALSPTDMLEVLATTHSAMHV
                     ARMLEKLDQQSFPLLRQLGLDMPQRPHIARRIEDTVSDEGEVLDTASAALRKLRFDIR
                     GANQRLQDRLRTLVHEFGSALQEPIVTMRSDRYVIPVRAESRGQVRGIVHDQSASGAT
                     VFIEPMVVVELNNRLRELQIAERQEVERILRALSLEIGQEADALRLDVELLAEFDLHL
                     AKARYGRMTRCSEPLLNDQGHIDLREARHPLLTGKVVPINFHLGNEFFIVVITGPNTG
                     GKTVALRTVGLLTLMAQAGLYIPADEHSELTVFEEVFADIGDEQSVEQNLSTFSSHMT
                     RIIEILRRIDEEQQREQPAIRGRPSETLTGKHENDRLRALVLFDELGAGTDPSEGSAL
                     ARAILTFLLERHVTTIATTHYTELKAFAHEQPGVVNASVEFDIETLSPTYHLSIGLPG
                     RSNALAIANRLGLDLRIIEQSREYLGSAGVRMENLLEGLQEERKSLADEKFHLSMERA
                     EAEYQRKQLEQDRHTLEEQRAQILNDARSQALRELDEMQRTLGKIRVESSRSSMTRER
                     LGEARETVRRLEENIPAAIPAPRKAAKQAEPELEAVGGPLQIGDTVRVLSFGQNADLV
                     GLSPDRGEAEVQMGALRFRVSTDNIERLSKRQSAQETRETSATRPARSEPEKDQPALT
                     LPRYEDQPEVATQLDIRGWRVEQALEELESYLNDAVMSGMPSVRIVHGKGTGALRAAV
                     REQLTHHPLVKSYTTAAPQEGGDGVTIVRLNV"
BASE COUNT          758 a         1194 c         1274 g          875 t
ORIGIN      
        1 cggtcgaagg gctgacagat ccttcgctgc gctcaggatg acagggccgt tattgttttg
       61 ggaaacttca tcaccgctcc tacggaatgg gttaatactt tatcagcgcg aagacatcgt
      121 ggccgcccaa ctgctcgcgg cctttgagga aggccagttc gaccaggaag gcgatgcccg
      181 cgatatggcc gcccagttgc tccaccagtt ccatcgcggc ggaggtggta ccgccggtgg
      241 ccaacaggtc atcgacgatc agcacgcgct ggccagggcg aatggcgtcc gcgtgcattt
      301 cgaccacgtt cgtgccgtat tccagcgaat actcgacgct gatggtctgg tgaggcagtt
      361 tgccgaactt gcgcaccggc acaaagccac aattcaatag ataggccagc gccgcgccaa
      421 aaataaagcc gcgcgattcg acgccgacga cgacctggat gcccgaaccg gcataatggg
      481 cggcgagacg atccatcgcg gcatgaaagg ctttggcgtc ctgcagcagg ggggttatat
      541 ctttgaagag gatgcctttc tgtggaaaat cagggatgtc tcgaatccag tcttccaggc
      601 gaatagactc aggtgctgac gacatcggat gcgttctcct ctactaacac agtcatacat
      661 tttgtcggct ggcccgctta cctgcaagcc gtaagcagta taccccagca tatgcagacg
      721 cgcaaggatt tcagggaagt tcacccaaac ttccctgaaa actatggcgt aagcggctgc
      781 acttgcgacg tacagttcgg gcagcgcgac gcctgtatcg ggatgctgct gaagcagtag
      841 gggcaatcac gcatcgtcgc gggggctggc acctctttcg gcttgaaacg agccatcagc
      901 gtattgacgg gtaatacaac aaagtagtag atcacaaggg cgatgatgag gaagctgatg
      961 atcgcgttga tgaaggtacc gatgttgagc ggtttatggg tccaggcaat ggtaatgaac
     1021 caggtggaga gactaccacc aggagcaggt atgagtgggg tgatgacgtt gctgacgagg
     1081 gaagtgacga ccccactgaa agccgcgcca atgacgatgc cgacggccag gtcaacgacg
     1141 ttgccgcgca ggatgaacgt cctgaagccg cccagtgtgc ctgtggcgga tcgctccaat
     1201 cgtcgaattc tttctgaagc catagttaca ggtcctccat gttgaaatag atgtgccagc
     1261 acgtgttgac tccctctacg gagacgcgca tggcacagta gaacgatcaa tttccatgta
     1321 attacacgtt gagccgcacg atggtgacgc catcacctcc ttcttgcgga gcggcagtcg
     1381 tatacgactt cactaacggg tggtgcgtca gttgttcgcg taccgcagcg cgcagcgcgc
     1441 cggtgccttt gccatgcacg atgcgcacgg aaggcatccc cgacatcacc gcgtcgttca
     1501 ggtagctttc cagctcttcc agcgcctgtt ccacgcgcca gccgcgtatg tcgagctgcg
     1561 tggccacctc cggctggtct tcgtagcgcg gcagcgtcaa ggcgggctga tccttctccg
     1621 gctcggagcg cgctggtcgg gttgctgacg tctccctggt ctcctgcgct gactggcgtt
     1681 tgctgagccg ctcgatgtta tccgtgctga cgcggaagcg cagcgccccc atttgcacct
     1741 cagcctcgcc gcggtcgggt gaaagtccga ccaggtccgc gttctggccg aagctcaaga
     1801 cgcgcacggt atcgccgatt tgcagcggcc cgccgaccgc ttcaagctcc ggctcggcct
     1861 gtttcgccgc tttgcgtggg gccggaatgg ccgccgggat attctcttcg agcctgcgca
     1921 ccgtttcgcg cgcctcaccc aggcgctcgc gcgtcatact cgagcggctg gactctaccc
     1981 tgatcttgcc cagggtacgc tgcatttcgt ccagttcgcg cagcgcctgc gagcgggcat
     2041 cgttgagtat ttgcgcgcgc tgctcttcga gcgtatgccg gtcctgttcc aactgcttgc
     2101 gctggtactc ggcctcggcg cgctccatgc tcaggtggaa cttctcgtcc gccaaggatt
     2161 tgcgctcctc ctgcagccct tcgagcaggt tttccatgcg cacacccgcg ctgcccaggt
     2221 attctcgcga ctgctcgatg atgcgcagat cgagacccag gcgatttgcg atggcgagcg
     2281 cattgctgcg tccgggcagg ccgatgctca ggtgatacgt cggcgagagc gtctcgatat
     2341 cgaattcgac cgaggcgttg acgacgccgg gctgttcgtg cgcgaaggct ttcagctcgg
     2401 tgtagtgcgt ggtcgcgatg gttgtaacat ggcgttcgag caggaaggtc aggatggcgc
     2461 gcgccagtgc cgatccttcg cttggatcgg ttcccgcgcc cagttcatcg aagagcacca
     2521 gggcgcgcag gcggtcgttc tcgtgtttgc cggtcagcgt ctcggagggc ctgccgcgaa
     2581 tggcgggctg ctccctttgc tgctcctcat cgatacgacg caagatttcg atgatccgag
     2641 tcatgtgtga cgagaaggtg ctgaggttct gctcgacgct ctgttcgtcg ccaatgtccg
     2701 cgaagacttc ttcgaagacg gtaagctctg aatgctcgtc cgccgggata tagaggccag
     2761 cctgagccat gagggtcagc aggccaaccg tgcgcagtgc aacggtcttg ccgccggtat
     2821 tggggccggt gattacgacg ataaagaact cgttgcccag gtggaagttg atcggcacga
     2881 ccttgccggt cagcagggga tggcgcgcct cgcgcaggtc aatgtgcccc tggtcattca
     2941 gcagcggctc ggagcagcgc gtcatgcgcc cgtagcgcgc cttggccagg tgcaggtcaa
     3001 actccgccag cagttctacg tcgagccgca gcgcgtcggc ctcctgcccg atttccagcg
     3061 agagggcgcg caggatgcgc tcgacctcct ggcgttccgc gatttgcagt tcgcgcagtc
     3121 tgttattgag ttcgaccacg accatcggct cgataaagac ggtcgcgcca ctcgccgatt
     3181 ggtcatgcac gatgccgcgc acctggccgc gactctccgc ccggacagga atgacgtagc
     3241 gatcgctgcg catggtgacg atcggctctt gcagtgcgct gccgaactcg tggacgaggg
     3301 tgcgcaggcg gtcttgcagg cgctgattgg ccccgcgaat gtcgaagcgc agcttgcgca
     3361 gagcagcgct ggcggtatcc agcacttcac cctcatcact gacagtgtct tcgatgcgcc
     3421 gcgcaatgtg ggggcgctgc ggcatgtcca ggccgagctg gcgcagcagg ggaaagctct
     3481 gttgatcgag cttttccagc atgcgggcaa cgtgcatggc gctgtgcgtg gtcgccagca
     3541 cttcgagcat gtcggttgga ctcagcgcgc cctctcgcgc ggcgcggctc aggtgcgcgc
     3601 ggatatcgcg tgcgccctgt acgcccgcgt cggcgcgcag gtcgatgagc ttatacgcct
     3661 cggaggtgta ggccagcctg cgacgcgcct cgtcgatatt tggcgtcggc tctaatgctc
     3721 tgaccagttc tttgctggcg gagaatgctg cctctttcgc tacgcgctct aaaatcttgg
     3781 gaaattccag cgtttgaata cttttctcgt gcatgatgat gtgaatagtc cttgtcttgg
     3841 gaagcggaca acagcagatg gctgccgccg gttggttctc gttggtcgtt tatggcagat
     3901 tcttcacggt gctgcttatc cagtgatgcg ctaacataag ataccatatt tgcgccagga
     3961 atgtaagatg ggttggaact tcgcccagtg tgtcgcgtat actactatta gagaacggaa
     4021 acaaatcaaa ggagcagtgg ctcatgatcc tctatcaagg cgagcgggta tattggggcg
     4081 caccggaagt catttacctg g
//
LOCUS       DASZAK010001336         3102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_176189, whole genome shotgun sequence.
ACCESSION   DASZAK010001336 DASZAK010000000
VERSION     DASZAK010001336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176189"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="VGT44_23395"
     CDS             complement(<1..367)
                     /locus_tag="VGT44_23395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEV2583819.1"
                     /translation="MRVISDVQEMMDAHPAWASPDDVGFVPTMGYLHEGHLALVRQAR
                     RENDVLAASIFVNPAQFGPHEDLNAYPRDLQRDLRLLEDAGVDMVFTPTPEAIYPSGF
                     VTYVDPTGPLVTEAEGASRP"
     gene            complement(486..1181)
                     /gene="rpiA"
                     /locus_tag="VGT44_23400"
     CDS             complement(486..1181)
                     /gene="rpiA"
                     /locus_tag="VGT44_23400"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV2583820.1"
                     /translation="MQHSQQDEWKRMAGEAAVTYIEPGMVIGIGTGSTAVCMIRALAQ
                     RLREGLTIVGAIPTSRATAQLATSLGIPLTDLDSYPEPDLAIDGADEIDPHLFLIKGG
                     GGALLREKIVASAARRFLVIADPTKLVAKLGSLAPLPVETVPFATATVYRRLEQLGAE
                     VTLRQRDGKPFITDNSNVILDCSFPQGIDDAPALQARIRGIVGVVETGLFLHMTERVI
                     IGDTGGVREMRAL"
     gene            1356..>3102
                     /locus_tag="VGT44_23405"
     CDS             1356..>3102
                     /locus_tag="VGT44_23405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2583821.1"
                     /translation="MAQQWRILVVEGDEALNRSIVATLAQDGYSVQGVATGGEAIKIL
                     WANEPHLMIGDRALPDADGFDLLQWIRSYRPKTRMMMLATPGSSGRRTRALEQGAVAY
                     FEKPIDLLLLKVEVRRLLQQTGFTANLDSFDLLDVIQIINMSRKSIALVVHTGLEEQG
                     MLGFQEGEMIWAEYGTLRGEEAFFALAAYKNGMVTQQPWNNVARPNVNQPLSRLIMQA
                     LQYRAKYEAAQQVSGEHKVFDKPFTMQGDSEGASPLDALLTEAPDDRPFVFVAENDGD
                     ASQGEPATPMQPAASMASSAPQAAAQDEPRPWWQESAKVPRALRPTPTENASATKSNG
                     STAQETPRAGVEKMVEGTSAENAAILTNGSSGVSITPSTVRKTPTGQRSDLPSWLMDQ
                     PTQFEVQAVRPSALKGSGQIPVTPLTPTARPAQSSSTPRKTDELRREQAVSASVTVNA
                     SNSARPALTALMTPTPASATFGEVVVAPAKMPVNFPFAASATQQVREQPKQAQPPRKR
                     NYAALVSALETVGYALPGFIAAALVGLDGQPIAQVATDDLDITGICATLSALLQGAVR
                     ALDTGVFGQHEDTIIT"
BASE COUNT          674 a          934 c          923 g          571 t
ORIGIN      
        1 cggggcgact ggccccctcg gcctcggtca caagcggccc cgtgggatcg acataggtca
       61 cgaacccgct gggatagatc gcttcgggtg tcggagtaaa gaccatatcg actccggcat
      121 cttcaagcag tcgcaggtcg cgttgtaaat cgcgcgggta cgcattcaaa tcttcatgcg
      181 gcccgaactg cgccggattt acgaaaatgc tggcggccag cacgtcattc tcgcgacgcg
      241 cctgccgcac gagcgcaaga tgtccctcgt gcaggtagcc catcgttggg acgaaaccca
      301 catcgtcggg gctggcccat gccgggtgcg catccatcat ctcttgaaca tcgctgatga
      361 ctcgcatacg ctcaccactc actccatcac tatcggcacg gaaatcatga cgtagtatac
      421 catgtcgaag aatggtaatg tcaccctgag cgccgcgaag ggtctgttga ttgcccatga
      481 tgaattcata aagcacgcat ttctcgcacc ccgccggtgt cgccaatgat gacgcgctcg
      541 gtcatatgca ggaacaggcc ggtttcgacg acgccaacga tacctcgtat acgggcttgc
      601 agcgcgggcg cgtcgtcgat gccctgcggg aaagagcagt ccaggataac gttgctattg
      661 tcggtgatga agggcttgcc atcgcgctgg cgcaacgtca cctctgctcc cagttgttcc
      721 aggcggcgat acacagtagc agtcgcgaac ggcaccgtct cgacgggcag gggggcgagg
      781 ctgcccagct ttgcgacaag tttggtcggg tccgctatga cgagaaagcg gcgcgcggcg
      841 ctggcgacaa tcttttcgcg cagcagcgcg cccccgccgc ccttgatgag gaagaggtgc
      901 gggtcaattt cgtccgcccc atcgatggcc aggtcaggtt ccgggtagct atccaggtcg
      961 gtgagtggaa tacccaggct ggtggcaagc tgcgcggtgg cccgcgatgt aggaatagcc
     1021 cccacgatgg tcagtccctc gcgcagccgt tgagccagcg cgcgtatcat gcagacagcc
     1081 gttgagcctg tgccaatacc gatcaccatg ccgggttcaa tatacgtgac agccgcctcg
     1141 ccggccatac gtttccattc gtcctgttgt gagtgctgca tcgcattgct ccaatatcca
     1201 tccaaaaatg acgccgaaat atactccatt atcgcatgtc ccgcctggat ttttcacgga
     1261 tgagataccc cttgtcaacg aggcggtctc atgttacact ctgtgagtac aaagaaaagc
     1321 aacagcttcc tatcttattt ttggtggtgt caagcatggc gcagcaatgg cgtatcctcg
     1381 ttgtcgaggg ggacgaggcc ctgaaccgca gtattgtcgc gaccctggcg caagatggct
     1441 atagcgtaca gggtgtggcg acgggcggcg aggcaatcaa aatcctctgg gccaacgagc
     1501 cgcacctgat gattggagat cgagccttgc cggatgcaga cggcttcgac ttattgcaat
     1561 ggattcgctc ctatcgcccc aaaacgcgca tgatgatgct cgccacgccc ggctcgtcgg
     1621 gaagacgcac acgcgccctg gaacaagggg cggtggcgta tttcgagaag cccatcgacc
     1681 tgctcctgct caaagtagaa gtgcgccgct tgctgcaaca gaccggtttt accgccaacc
     1741 tggactcctt cgacctgttg gatgtcatcc agatcatcaa tatgagtcgc aaaagcatcg
     1801 ccctggtggt gcatacgggc ctggaagaac agggaatgct gggcttccag gaaggcgaaa
     1861 tgatctgggc ggaatatggt acgctgcgcg gcgaagaagc ctttttcgcc ctggcggcct
     1921 ataagaatgg catggtcaca cagcagccct ggaacaacgt cgccaggccg aacgtcaacc
     1981 agccgctgtc gcgcttaatc atgcaggcgc tgcaataccg tgccaagtat gaagctgcgc
     2041 agcaggtgag cggcgagcat aaggtcttcg acaaaccatt cactatgcag ggcgactccg
     2101 agggagcatc gccgctcgat gcgttgttga ccgaagcgcc cgacgatcgg ccatttgtgt
     2161 ttgtggcgga aaatgacggg gacgcgagtc agggggagcc agcaaccccc atgcaaccgg
     2221 ccgcgtcgat ggcatccagc gctcctcaag cagcggcgca agacgaaccc aggccctggt
     2281 ggcaagagtc agccaaggtg ccacgcgcgc tgcgcccaac gcccaccgag aacgcaagcg
     2341 cgacgaaaag caatggaagc actgctcagg agacaccgcg cgccggcgtg gagaaaatgg
     2401 tggaagggac gagcgcagag aacgccgcca tacttacgaa tggctcaagc ggagtgagca
     2461 tcacaccgtc cacggtgcgc aaaacgccga caggccagcg ctctgatctg ccatcgtggt
     2521 tgatggatca accgacacaa tttgaagtac aggcggtgcg tccatccgcc ctgaagggtt
     2581 ccggccagat tcctgtcacc ccgcttacgc cgaccgccag gcccgcgcaa agctcttcaa
     2641 cgcccaggaa aaccgatgag ctacggcgcg agcaggcggt cagcgcgagc gtgacggtga
     2701 atgcctcaaa cagtgcccga ccagctctga cagctttgat gacccccacg cccgcgtcag
     2761 ccacattcgg cgaggtagtc gtagcaccgg ctaagatgcc ggtaaacttt ccgtttgctg
     2821 cttctgccac ccagcaagtg agggaacagc cgaaacaggc ccaaccgccg cgcaaacgca
     2881 actatgccgc gctcgtttcc gccctggaaa cggtcggata cgcgctgccc ggcttcattg
     2941 ccgcggcgct ggtgggactg gacggtcagc cgattgcgca ggttgctacc gacgacctgg
     3001 acatcaccgg catttgcgcc acgttgagcg cgctcttgca aggagcagtg cgggcgttgg
     3061 acacaggcgt gtttggacaa cacgaagata cgattattac ca
//
LOCUS       DASZAK010001337         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_139122, whole genome shotgun sequence.
ACCESSION   DASZAK010001337 DASZAK010000000
VERSION     DASZAK010001337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139122"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VGT44_23410"
     CDS             complement(<1..345)
                     /locus_tag="VGT44_23410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2583822.1"
                     /translation="MFNSVAATVLFVRDLARCTAFYRDTLGLEVTESTSELASFHMEN
                     VYFLLLEVSVAAHMVSEEALDLNIEGGPRGLLAAGVEDIDAAYETLKARGVTFLRPPT
                     DQPWGLRTAYFAD"
     gene            complement(489..1178)
                     /locus_tag="VGT44_23415"
     CDS             complement(489..1178)
                     /locus_tag="VGT44_23415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583823.1"
                     /translation="MKKILVVEDSEPNVYLLGQTLRTNGYEVLVALKGKDALESLERQ
                     HIDLVLLDLILPDMSGHDVCKAIRVVDPNLPIIILSVKGDERDKVRALQAGADDYLAK
                     PFSTLELLERIKVQFKHTRRFKVEPDTVRFIAGPLEVDFEQRLVKVHGQEIDLTYTEF
                     ELLNILVRNPGRYVTYDFILSNVWNDEDYSERKNIHVFINRLRKRIELPAGRRYIYNE
                     PKVGYRFLVEE"
     gene            complement(1198..3006)
                     /locus_tag="VGT44_23420"
     CDS             complement(1198..3006)
                     /locus_tag="VGT44_23420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583824.1"
                     /translation="MDTLREEQGVMLFFLRHQLSTGNGLRRSWKRYAKDTLFAAMVAA
                     VLTVPIMLLQSLVNASAMLLFYLAVVVVVAYWRSFRFAVLTAFLTCFICDYFLVSPSD
                     SLLINDPKAGLDLLAYLFCAIVLSFLASRLRDHTVKIKQQKDEEQVRYSVEVSRQAQQ
                     MSAYWAVMQQIREEKELQKQLELLAHTIEELFACCGVRKCTFFGPDIDGQQFLHRWNE
                     DSGPFAGLSTLEEDSAAWVLKHGQSVEVRETPMIAREMGSYLRRVVASNGTKKASQRR
                     DALCSYLVPLLSGQKVLGESGQKVVGVMRLLIEDSDHPDAQVIKKRLGIGCASSEEQE
                     NLFSQLLGHATNLVEQALIERALMEHESQNQELQRRSDALRSTIISSVSHDFHTPLNF
                     IKGVATGLQVRDVQSIEAAEHSQMMELVVNEVDWLERMVMKMLDLSRIEQGALKIEKE
                     LYPIDGIILTTLDRAHMRSLLAGRRVDRHVPDELTPVEVDPLLIEHVLVNLLENAVRY
                     TPEQSPIAIGVREVEGWLIVSVTDSGPGIPKLELERIFESFYRVKSRGAGDVPAAPGS
                     GLGLAVCRGFVEAHGGSIWAENRTGGGAVMQFTLPL"
     gene            complement(3041..3610)
                     /locus_tag="VGT44_23425"
     CDS             complement(3041..3610)
                     /locus_tag="VGT44_23425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2583825.1"
                     /translation="MVSTLEPTLPVKRAQEGDQEAFATLVEHFEHEIRSYLIVHLGDY
                     DEANDAVQQVFLKAWLNLDSLHDMACFRFWLYAIVKHQVCDYWRKKRVRTQSWEDLEL
                     DNRLAGLPGPEDNAERAELIRLTLHTLSTKQRRCLVLQNEGFTPDEIAPIVGISSASV
                     YTYLCAARRQFRATYRQLEDESHSVLLTV"
BASE COUNT          873 a         1108 c          939 g          882 t
ORIGIN      
        1 atcggcaaag tacgccgtgc gcagtcccca gggctggtcg gtcggtggtc tgaggaacgt
       61 gactcccctg gccttgagcg tctcgtaggc ggcgtcgata tcctcaacac cagcggctag
      121 aagcccgcga ggtcctccct cgatattcag atcaagcgcc tcctcgctga ccatatgcgc
      181 ggcaactgag acctccagca ggagaaagta tacattttcc atatggaagg aggcaagctc
      241 ggaagtactc tctgtcacct caagcccgag cgtgtcccga taaaacgccg tacacctggc
      301 cagatctcgg acgaacaaca ccgtcgcggc aacactgtta aacatggttt tcacctattc
      361 tttcattgtc gagtcaacac agaaacaact cctggtcttt ttgcctattc tttggcacag
      421 tagaaaaaat ggagggtgat acttcctacg caaaccatcc tcttcatatg gtacataata
      481 ccgcattctc actcttccac gagaaagcga taccccacct tcggttcgtt gtagatataa
      541 cgacgtccgg cggggagttc gatgcgttta cgcaggcggt tgatgaagac gtggatattt
      601 tttcgctcgg aatagtcttc gtcgttccag acattggaga ggatgaaatc ataggtgacg
      661 tatcttccgg ggttccttac caggatgttc agcagctcaa attcggtata cgtcaggtcg
      721 atctcctgcc catgcacctt gacgagtcgc tgctcaaaat ctacctccaa tggtccggca
      781 atgaatctca cggtgtcagg ttccactttg aagcgcctgg tatgtttgaa ttgaacctta
      841 atacgctcga gtaactcgag cgtggaaaat ggctttgcca ggtagtcatc cgccccggct
      901 tgcagagcgc gaactttatc gcgctcatct cccttgactg acagaatgat gatcggcaga
      961 tttgggtcaa cgacacggat ggccttacag acatcgtggc cgctcatatc aggcaagatc
     1021 aggtcgagca gcaccaggtc aatatgctgg cgttccaggc tttcgagcgc gtctttccct
     1081 ttcaatgcga cgagtacctc gtatccattg gtgcgcagcg tctgcccgag caagtatacg
     1141 tttggctcgc tgtcttcgac aaccagaatt ttcttcaccg gctgctccct cggcctttca
     1201 taagggcaaa gtaaactgca tcaccgcgcc gccaccggtt cgattctccg cccagatact
     1261 cccgccatgc gcctctacaa agccacggca gactgccagg ccaagccctg atcccggcgc
     1321 cgcaggcaca tcgcccgctc cacgcgactt gacacgataa aagctttcaa aaatacgttc
     1381 gagttcgagt ttgggaatgc caggtccgct gtcggtcacc gagacgatca gccacccctc
     1441 gacctccctc acaccaatgg cgattggcga ctgctccggc gtataacgca cggcattttc
     1501 aagcaaattt accagcacat gttcgatcaa caagggatcg acctcgaccg gcgtcaactc
     1561 gtccggcaca tgccggtcaa cgcgccgccc tgccagcagc gaacgcatat gcgcccggtc
     1621 gagcgtagtg agaatgatgc catcgatggg atacagttcc ttttcgattt tcaacgcgcc
     1681 ctgctcgatg cgcgagaggt cgagcatctt catgaccatg cgctccagcc agtctacttc
     1741 gttgacaacc agttccatca tctggctgtg ttcggcagct tcgattgatt ggacatcgcg
     1801 cacctgcaaa cccgtcgcga cgcccttgat aaaattcagc ggcgtatgaa aatcgtgcga
     1861 taccgaggaa ataatcgtcg aacgcagcgc atccgaacgg cgttgcagtt cctggttctg
     1921 gctttcgtgt tccatcaaag cgcgttcgat cagggcctgt tcgaccaggt tggtagcatg
     1981 gccgagcagt tgcgagaaca ggttctcctg ttcctccgaa gatgcacaac cgatgcccag
     2041 ccgctttttg attacctgcg catcaggatg gtcgctatcc tcgatcagca gacgcatcac
     2101 ccccaccact ttctgtcccg actcgccaag cactttctgc cctgaaagca ggggtaccag
     2161 gtaactgcac agggcatcgc gccgttgact cgcctttttc gtgccattgc tggcaacgac
     2221 tcgccgtaga tagcttccca tctcacgcgc gatcatgggc gtctctctta cttccacgga
     2281 ctggccatgc ttcaaaaccc aggcagcgct atcctcttcc agtgtcgaaa gaccggcaaa
     2341 tgggccggaa tcttcgttcc aacgatggag aaactgttgt ccatcgatat ccggtccaaa
     2401 aaatgtgcat ttccgcactc cgcaacaggc aaataactcc tcgattgtat gcgcgagcag
     2461 ctccaattgc ttttgcagct ccttctcttc cctgatctgc tgcatcaccg cccagtatgc
     2521 gctcatctgc tgcgcttgac gactaacttc tacgctgtag cggacttgct cctcgtcctt
     2581 ttgctgcttg atcttcacag tatggtcgcg caggcgcgat gccaaaaagc tcagcacaat
     2641 cgcacaaaag aggtaggcga gcaggtctaa acccgctttc ggatcgttaa tcagtagaga
     2701 gtcagagggt gacacgagaa aatagtcgca gatgaagcac gtgagaaatg ctgtcagcac
     2761 cgcgaagcgg aagctgcgcc agtaggcgac caccaccacc acagcgaggt agaataacag
     2821 catcgcactc gcatttacga gcgattgcag cagcatgatt ggaacggtca agacggctgc
     2881 gaccatcgcg gcgaacagtg tatccttcgc atagcgcttc caggagcgcc tcagcccatt
     2941 gccggtcgaa agttgatgac gcaagaagaa caacataacg ccctgttcct ctctcaaagt
     3001 atccatggac aagctcaata ttgagcataa atcatacgag ttacactgtc aatagaacag
     3061 agtggctttc gtcttccagt tgccggtagg tagcgcgaaa ctggcggcgc gctgcgcaga
     3121 gataagtgta gacgctggcg ctgctgatgc caacgatcgg ggctatttcg tccggcgtaa
     3181 acccctcgtt ttgcagcacc aggcatcgcc gctgcttggt cgagagcgtg tgcagggtga
     3241 gacgaatcaa ctcggctcgc tcggcattat cttccgggcc tggcaaacct gccagcctgt
     3301 tatccagctc caggtcctcc catgattggg tgcgcaccct cttcttgcgc caataatcgc
     3361 acacctggtg tttcacgatg gcgtagagcc agaatctgaa acaggccata tcgtgcagtg
     3421 agtccagatt cagccaggct ttcagaaata cttgctggac cgcgtcattt gcctcgtcat
     3481 aatcccccag atgcacgatc agatagctac ggatttcatg ctcaaaatgt tcaaccaggg
     3541 ttgcaaatgc ctcttgatct ccctcctgcg ctcgtttgac cggcagggtc ggttccaggg
     3601 tcgataccat gctgctcttc ctttcttccg ctcccggcaa tattgctgtg atattgccac
     3661 gcaggaagag catacacgag ggatatgtat tccctctaaa ttctagacga aagttcttac
     3721 aattctctaa attttataag gatgcggtga tgaacgtttc caaaacaaga tcggtcatag
     3781 tttgtcaccc tgagcgcagc ga
//
LOCUS       DASZAK010001338         3646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_69112, whole genome shotgun sequence.
ACCESSION   DASZAK010001338 DASZAK010000000
VERSION     DASZAK010001338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69112"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VGT44_23430"
     CDS             <1..825
                     /locus_tag="VGT44_23430"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEV2583826.1"
                     /translation="GGSGGEWGGDPCGRPGGIQLHRRLFMLNYSEQLQDAAELIEQAR
                     RIVALTGAGISTESGIPDFRSAGSPWVAQPPVSYKEFISNAEARQQYWRTRRQLSGQV
                     SSARPNAAHDALAVLERRGKLLGVITQNFDGLHQDAGNAAERVVELHGTSREAACTLC
                     GARSPMADLQRRVDAGEVDPTCALCGGYMKTATILFGQRVPDAVLARAKTLATECDLF
                     LVVGSSLQVTPAANLPRLALSRDMPLIIVNLAPTPLDDYADVVIHGKAGEVLPTII"
     gene            complement(860..1498)
                     /locus_tag="VGT44_23435"
     CDS             complement(860..1498)
                     /locus_tag="VGT44_23435"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HEV2583827.1"
                     /translation="MSDLLDLYDYRMRVATMYRERNRAMLDGDDAKQVCDRFRATRNA
                     LFKAHPQSALDAEQRAQFEGLRYFPYVPSLCVEAEVDMQVEPAVLTVAMNAEELMTMT
                     RVARLHFTLEGQRKSLAMYWLNVYGGGLFLPFRDASCPEESYGGGRYLFDTIKGSDFL
                     QADGVSHIERILLDFNYAYNPSCAYNSRWVCPLAPVENRLDIAIRAGEQNFH"
     gene            1626..2462
                     /locus_tag="VGT44_23440"
     CDS             1626..2462
                     /locus_tag="VGT44_23440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2583828.1"
                     /translation="MRMSARGKPHRIAYGPEYLQFGELYVPRTPDAHDPYPVILLIHG
                     GYWRARYDLTLMTDLARDLAERGYAAWNIEYRRVGDAGGGWPGTFFDVARAADHVQTI
                     APGYNLDVRRVVAIGHSAGGHLACWLAARRRIPANSPLAPGAQDGSPATLALAGVVSQ
                     AGVLNLETAWKLHLSNDAVVELLGFSWPDAPDRYLAASPAALLPLGVPQIILHGTRDA
                     HVPIEISRDYAAKALAARDPVEYIELEGADHFDLIHPRSQAWSVTLEALKKLLMHISS
                     SL"
     gene            complement(2478..3194)
                     /locus_tag="VGT44_23445"
     CDS             complement(2478..3194)
                     /locus_tag="VGT44_23445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583829.1"
                     /translation="MNFPFQIGLPQVVLLLIVLVGLWLFITALTGLVRRYEIHELKEE
                     EKKAGMRAPRRWGYGRLRWKRGGSGILLLIIAISLLWLTFLVQTYLGLTGDIRVARIH
                     ATATNIPHLMSVEVILYDNNGNPASDKTYIVQGDEWMLQGDIVKFPSWLNILGLHSGF
                     KLTRLEGRYDDVNMERTAPHTVIELNGGDDNFFKTVQAQAWTSPFVDSAYGSSTFLSP
                     DNHTYDIFVSQDALHPEPVK"
     gene            3454..>3646
                     /locus_tag="VGT44_23450"
     CDS             3454..>3646
                     /locus_tag="VGT44_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEV2583830.1"
                     /translation="MILTGRLLTWWMHLPPAETHDIVITRDRQIPMPDGTVLLADHYA
                     PRTSPKLPTLLIRSPYGRRG"
BASE COUNT          740 a         1047 c         1081 g          778 t
ORIGIN      
        1 ggcgggagcg gcggggagtg gggtggagac ccttgcggtc gccctggggg tatacaattg
       61 caccggagac tattcatgct caactacagt gaacaactgc aagatgcggc tgaactgatc
      121 gaacaagcgc ggcgtatagt ggcgctgacg ggcgcgggca tcagcacgga gtcgggcatt
      181 cctgattttc ggagtgctgg gtcgccctgg gtggcgcagc cacccgtcag ctataaggag
      241 tttatctcca acgcagaagc gcgtcagcaa tactggcgga cgcggcggca gctctccggc
      301 caggtgtcga gcgcgcggcc taatgcggcg cacgatgccc tggcggtact ggaacggcgc
      361 ggcaagctgc tcggcgttat cacgcagaac ttcgatgggc tgcaccagga cgcgggcaac
      421 gcggcggagc gcgtcgttga attgcatggc acatcacgcg aggcggcctg tacgctgtgc
      481 ggcgcgcgct ccccgatggc ggatttgcag cggcgcgtcg atgcgggcga ggtcgatcct
      541 acctgtgctt tatgtggtgg ctatatgaaa acagcgacga tcctctttgg ccagcgcgtg
      601 cctgacgcgg ttctggcgcg cgcgaagacg ctggcaacgg agtgcgatct gttcctggtg
      661 gtcggctcat ctttgcaggt cacgcccgcc gcgaacctgc cgcgcctggc gctcagccgc
      721 gacatgcccc tcatcatcgt caatctggcg cctacgccgc tggatgatta cgccgatgtc
      781 gtcattcatg gcaaggcagg cgaagtactg ccaacgatca tttgaacgat tcatgagacg
      841 aaagatacct cgtgtcgctt caatggaagt tttgctcgcc cgcgcgtatg gcgatatcca
      901 ggcggttctc caccggagct aaagggcaga cccagcgtga gttgtaggcg caggacgggt
      961 tgtaggcgta gttaaaatcc agcaggatgc gttctatgtg ggatacgccg tcagcctgta
     1021 aaaagtcgct gcctttgatc gtgtcgaaaa gatagcgccc gccgccgtag ctctcctccg
     1081 ggcaggaagc gtcgcgaaac ggcaagaaca ggcccccacc gtagacgttg agccagtaca
     1141 tcgccaatga tttccgctga ccctcaagcg taaagtgcag gcgcgctact ctcgtcatgg
     1201 tcatcaattc ttcggcattc atggcgaccg tcaacacggc tggctcgacc tgcatatcga
     1261 cctcggcttc gacacacaaa gacgggacat agggaaaata gcgcaatccc tcaaactgcg
     1321 cgcgctgctc agcatccaac gctgactgtg gatgcgcttt gaataaagca tttctagtcg
     1381 cgcgaaaacg gtcacagacc tgcttcgcat catctccatc gagcatagcc cggttgcgct
     1441 cgcggtacat ggtggcgacg cgcatacgat aatcgtagag gtcgagtaaa tctgacattg
     1501 gtttcctttc gtcactactt ttgctttcgc ctacgatacg aatatgaata ctatcctaca
     1561 gtcaatctat acgctgaaat gtttcatatg gacttcatgg tatcattcaa gcaaaggagg
     1621 gtggtatgag aatgtctgct cgcggcaaac ctcatcgcat tgcgtatggg cctgagtatc
     1681 tgcaatttgg tgagttatat gtgccgcgaa cgccggacgc gcacgatccg tacccagtta
     1741 ttctgctgat tcatggcggg tactggcggg cgcgttacga tctaacgctg atgaccgacc
     1801 tggcgcgcga tcttgccgag cgaggctatg ccgcctggaa tattgaatac cggcgggttg
     1861 gcgatgctgg cggcggctgg cccggcacgt ttttcgacgt ggcgcgcgcc gccgatcacg
     1921 tgcaaacgat cgcgccaggc tataacctgg atgtgcggcg tgtcgtggcg atcggacatt
     1981 ccgccggggg acacctggcc tgctggctgg cagcgcgccg ccgcattccc gccaacagcc
     2041 cgcttgcgcc cggcgctcag gacggctcgc ccgcgacgct cgctctggcc ggagtggtca
     2101 gccaggcagg ggttctgaac ctggaaacgg cctggaagct gcacctaagc aacgatgcgg
     2161 ttgtagaact gctgggcttt agctggcccg acgcgcccga ccgctacctg gctgcttcgc
     2221 ctgccgcgct gctaccgtta ggcgtgccgc agatcatctt gcacggcaca cgagacgcgc
     2281 atgtgccaat cgaaatcagc cgggactacg cggcgaaagc gctggccgcg cgtgacccgg
     2341 tcgaatacat cgaactggaa ggcgctgatc acttcgacct gattcacccg cgctcacagg
     2401 cgtggagcgt cacgcttgag gcgttaaaga agcttttaat gcatatatcc agttcgttgt
     2461 aggtacaggg cgcaacatca tttcaccggc tcagggtgta gggcatcctg ggaaacgaag
     2521 atgtcgtagg tatggttgtc cgggctgagg aaggtgctgg agccataggc agagtcgaca
     2581 aagggtgagg tccacgcctg cgcttgcacc gttttgaaga aattatcatc acccccgttc
     2641 agctcgatga cggtatgggg cgcagttcgc tccatgttga catcatcgta acggccttct
     2701 aagcgggtca gcttgaagcc ggaatgcagc cccagaatgt tgagccagga cgggaacttg
     2761 acgatatcgc cctggagcat ccattcatcc ccctgcacga tgtaggtttt atccgaggcg
     2821 ggattgccgt tattgtcgta caggatcact tctacgctca tgaggtgcgg gatgtttgtc
     2881 gcagtggcgt ggatgcgcgc caccctgatg tcgccggtca accccaggta ggtctgcacg
     2941 aggaaggtca accagagcag cgaaatggcg atgatgagca agaggatgcc gctgcctccg
     3001 cgtttccagc gcaggcggcc atagccccac ctgcggggcg cgcgcattcc ggccttcttt
     3061 tcttcttcct tcaactcgtg tatttcgtag cgccgcacca gtcctgtgag cgctgtgatg
     3121 aacagccaga gtcccaccag tacaatcaac agcaggacaa cctgtggtaa accaatctgg
     3181 aaagggaaat tcaccgattg cttcctttct ttagcgtatg ctcttcttta cactacgatc
     3241 ccatacagca caatcatata ctgttcacac ggatttttca gcttttacgt tgcttcaacc
     3301 ttcccttgcg tagactcatt tgctcatggt acagtgtgaa cacatccatt tcggtccgta
     3361 ggcgcccctt ttcgtcattc cagagacgct tcacagacgg tccgggacgt ggtggtgaag
     3421 cgcggtacgt tgcagagcac aggagggtat ccaatgattt tgactggccg tctcctcacc
     3481 tggtggatgc acctgccacc ggctgaaacg cacgatattg tgatcacccg cgaccggcag
     3541 attcccatgc ctgatggaac cgtcctgctt gcggaccatt atgcgcctcg cacgagtcca
     3601 aagctcccaa cgcttctgat ccgctccccg tatggcagga gaggct
//
LOCUS       DASZAK010001339         2795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_283874, whole genome shotgun sequence.
ACCESSION   DASZAK010001339 DASZAK010000000
VERSION     DASZAK010001339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283874"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VGT44_23455"
     CDS             <1..476
                     /locus_tag="VGT44_23455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583831.1"
                     /translation="EVYLFAGTVADNIRYGKLDASRQEIIDAAKRAHAHDFIMALPHG
                     YDTDIGQRGVKLSGGQKQRLSIARVFIKDPPVIIFDEATSALDNESEKAVQDALEKLA
                     DRRTTLVIAHRLSTIRNAQRIVVLSDTGIAEQGTHAELLALNGVYANLYNMQSKI"
     gene            627..1442
                     /locus_tag="VGT44_23460"
     CDS             627..1442
                     /locus_tag="VGT44_23460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583832.1"
                     /translation="MIQALRYEPQGQRQRFEPPEQQEQIRHIHLHVHLNLTMRARQRR
                     ATHPNAIRLRRIFAALLAATVMPLGIFLFAMFTAADGPPPTAWENLMWVSSCLSIVAG
                     LALAWWAFGILIFQVWRESPRGRLGLVLPLYLLLLIPLAAALEQFGPLFLKLLFSIPL
                     LVVLIAYPLLTALLLVWVSREVRALREIAPTPSKLGFIAVAGMVLVLLGGLLWNLFFM
                     LSGGNTQLIFLPAIPIAIIVAICTLVRPPRSHGSMQAYPKDPSPFDTGSHEEP"
     gene            1510..2667
                     /locus_tag="VGT44_23465"
     CDS             1510..2667
                     /locus_tag="VGT44_23465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2583833.1"
                     /translation="MLQAQMHHATGPLKAGSLLAGRYHILDKIGEGGFGLVYKARDTR
                     YRSRLVAIKEIDLGTLSSRQIIEATDSYNREVTLLSRLEHRNLPRVYDHFTDPTHWYL
                     VLQYIKGETLEDYLKRIPGGLLPLKEVCKIGLQLSSVFGFLHVHKPPIIFRDVKPANI
                     MRTSRGRLYLIDFGIARRYNPQKSRDTGPLGSPGYAAPEQYGRAQSTEQTDIYGLGAT
                     LHTLLTGKEPYEDEQDTSPAQPQDRSDQEFQQLLDKMQAIDPADRPRSIGDVRNQLRI
                     IKIRHPLLRILRLKLLPFLIGLFIGSLPYTFLPFAGLAASNNDSLPFYLIPAALLFYF
                     WPVTLFSQIVTAIILYAARRRLMAIGIIIMLILIYLAILLGWLPSQASLVH"
BASE COUNT          584 a          837 c          748 g          626 t
ORIGIN      
        1 aggaggtcta cttgttcgcc ggaaccgtcg cggacaatat tcgctacggg aaacttgacg
       61 ccagccgaca agaaatcatt gacgcggcca aacgggcgca cgcgcatgat tttatcatgg
      121 cgttaccgca tggctacgac acggatatcg gtcaacgcgg cgtcaaactc tccggcgggc
      181 aaaagcagcg gttgagtatc gcgcgcgtct tcatcaaaga tcccccggtg atcatttttg
      241 atgaagccac gagcgcgctg gacaatgaaa gtgaaaaggc ggtgcaggat gcgttggaaa
      301 agctggccga caggcgcaca acgctggtca tcgcccatcg tttgtcaacc atccgcaatg
      361 cccaacgaat cgtggtttta tctgacaccg ggatcgctga acagggcacg cacgcggagt
      421 tactggcatt gaacggcgtc tacgcgaacc tgtacaacat gcaatcaaaa atatgagatt
      481 ggatggggag aatgcagtcc caaaggcaaa ggctagcgca gtggcagctg cactcgtgac
      541 gaggcgcttg ctcttgtcac tggccttgct ctggagtaca atagcgtgaa aagggtcccg
      601 gcacgagccg gggaaggaga tctgcgatga tccaggcgtt acgttatgaa ccgcaaggcc
      661 aacgccagcg ttttgaacca ccggagcagc aggagcagat caggcacatc catctgcacg
      721 tgcatctgaa cctgacgatg cgggcgcgtc agcggcgtgc cacccacccc aacgcgatcc
      781 gtctcaggag aatcttcgcc gccctcctcg ctgcgacggt gatgcctctc gggattttcc
      841 tcttcgcaat gttcacagca gcagacggcc cacctcctac tgcatgggag aatctgatgt
      901 gggtcagtag ttgcctcagt attgttgcgg gcctcgcact ggcctggtgg gcctttggaa
      961 tactcatctt tcaggtttgg cgagagagtc cgcgtgggcg cctgggcctg gttctcccgt
     1021 tatacctgtt actgctcatc cctcttgccg cagcccttga acagtttggc ccactttttt
     1081 tgaaactgct tttctcgatt cctctccttg tggtgctgat tgcctatcct cttctcactg
     1141 ctctcttgct tgtttgggtg agtcgcgagg tgcgcgctct tcgagagatc gcacccacac
     1201 ccagcaagct cggcttcatc gcggtggctg gaatggtact cgtgctgctc ggtggactcc
     1261 tctggaacct ctttttcatg ctgagcggcg ggaatacgca gctgatcttc cttcctgcga
     1321 tccccattgc catcatcgtc gccatttgca cgctcgtgag gcccccccgc tcgcacggga
     1381 gtatgcaggc ataccccaag gacccttctc cctttgacac tgggtcgcat gaggagcctt
     1441 gatggcatca tcttaaaacc ttgaccgcat cgtcgttacc ttctacaata ccctcagggg
     1501 atgagtctca tgctacaagc gcaaatgcat catgccacag ggccgctgaa ggcgggatcg
     1561 ctgctggctg gccgctacca catcctcgac aagattggcg agggtggctt tggcctggtg
     1621 tacaaggcgc gtgacacgag atacaggagc aggctggtcg ccatcaagga gatcgacctg
     1681 gggacgctca gttcccggca gatcattgag gccaccgact cgtacaatcg cgaagtgacg
     1741 ctgctttcgc ggctggaaca tcgcaacctg ccgcgtgtct acgatcattt caccgatcct
     1801 acgcactggt accttgtttt gcaatacatc aaaggcgaga cgctcgaaga ctatctcaaa
     1861 cgcattccag gaggtctcct accgctgaaa gaggtgtgca agatcggttt gcagctttcc
     1921 agcgtgttcg gctttctgca tgtccacaaa ccgcccatta ttttccgcga tgtgaaaccg
     1981 gccaatatta tgcgcacgag ccggggacga ctctacctga tcgacttcgg catcgcgcgc
     2041 cgctacaatc cgcagaagag tcgcgatacc ggcccgctcg gctcgccggg gtacgccgcg
     2101 ccggaacaat atggccgggc gcaatcgacc gagcagacgg acatctatgg cctgggcgcc
     2161 acgctgcaca cgctgctgac gggcaaggaa ccttacgagg acgagcaaga tacatcgccc
     2221 gcgcagccac aggaccgctc agatcaggaa tttcagcaac tgctggacaa gatgcaggcc
     2281 atcgaccccg ccgaccgacc ccgtagtatt ggcgatgtca ggaaccagtt gcgcatcatc
     2341 aaaatacgcc accctctctt gcgaatacta cgcctgaaat tgctcccttt cctgatcggg
     2401 ctgttcatcg gctcgctgcc ctacaccttt ctgccgttcg ccggactggc tgcctctaac
     2461 aacgactctc tgccgtttta cttgatacct gccgcactcc ttttctattt ctggccggtc
     2521 acactcttct cgcaaatcgt tacggcgatc attctgtatg cagctcgcag gcgtttgatg
     2581 gcgattggca ttatcatcat gcttatcctc atctacctgg cgatacttct gggctggctt
     2641 ccatcgcagg cgagccttgt ccactagacc aatctccgag gatgtccgaa tttggatcgc
     2701 cagcaggtcg attccgagca ttcccattcg ttgttatggc tgagcgttac aaaaataact
     2761 ttcacgagca aagccggtcc taacccgtag gcgcc
//
LOCUS       DASZAK010001340         5627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_34300, whole genome shotgun sequence.
ACCESSION   DASZAK010001340 DASZAK010000000
VERSION     DASZAK010001340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34300"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VGT44_23470"
     CDS             complement(<1..369)
                     /locus_tag="VGT44_23470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2583834.1"
                     /translation="MPQEKHILVVDDEPSIQHFVKRNLELRAFKVSLASNGLEALALF
                     EHANFALIILDIMMPGLDGLEVCRRIRQRSTVPIIILTALGEETDKIAALDQGADDYL
                     TKPFGVGELLARVKAVLRRNH"
     gene            complement(394..2361)
                     /locus_tag="VGT44_23475"
     CDS             complement(394..2361)
                     /locus_tag="VGT44_23475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583835.1"
                     /translation="MLRILERKIALQLLVFYGLFVIPLLLGGAELYFFQYGALQQSAQ
                     QADLGLAQAIAQNVEMQVQDTKGGSIDLAQLNARLKAEQQQLTTDGEVRIWILDSDGN
                     VIANTVGVSLHSNLLQRIPGLRDALHSKPGSVIAQDQQRDWLYSYVPVSGTSWAVAVG
                     RPTDVTFAAVISFQHSLIIALIMLLVGASVFWFVMHGWVVAPLGRLAQAATIMRPDQA
                     AKVTDGKQLAREKSRHDEIGRLVNAFSMMEEEMHTLFRKSDEQSFSRLQTLDAIMRSM
                     DEGVLLENPDGQIVYANHRLTQFAGFSTQELQLDSFQETHLTERLESLMEAPDTYREA
                     LHSAELGDGPHVIEFQVRGFYNQLGQLVPSRRLIRMRLFQVYDMANQLIGRGKIYHDV
                     TQQSEAEQIKKNLLAIISHELRTPLTAIKGYATSLLETDVELDATTQRDFLQRIVEEE
                     DRMAELVTSLLEMSQLEAGTLKLTPGYCRLDMLIQRAVDAAKCERLRIHVSLPEEIPV
                     LYIDQRRMEMVLRNILENAQQYATDLEDPGDKAEIEISARYALDDEDEGIYLSVTDHG
                     SGLPGDLTERIFERFYQVDGGRMRSSSGVGLGLSICRGFVEAHGGRIWAENRSGNNTN
                     DSGATFHILLPGRLLYVPGSRRVPFELEKAL"
     gene            2551..3879
                     /locus_tag="VGT44_23480"
     CDS             2551..3879
                     /locus_tag="VGT44_23480"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583836.1"
                     /translation="METLQLEKTEETSAREQLFPVIVASALGSAIEWSDFFYYSFLAV
                     TVFPTVFFSPLTPSIGMVASFSANFVGFVARPLGGVFFGRYGDRIGRKSTLAATLLLI
                     GATTMLMGVLPGYAQLGIAAPLLLVALRFLQGVGVGGEWGGSVLMTMEFSDQRRRGFW
                     TSWPQIGVPVGLALSSSSILLFKGLYPGEAFQAIGWRIPFLLSTILVAVGLYIRLRLP
                     ETPAFSRLIASRQQSVNITLHTWRLYWREILLSALIRTGEQAPFYIFTTFALSYGTLM
                     LHLSSTLIYTSMIAASGVSFCLMPCFGALSDRVGRRRTTIYGALAMMVCAFPFFLLLN
                     TGVPLLVILALALTLGGPHACLYGPQSSLIAERFPTRLRYTGASLGYQLASIIAGGPA
                     PIIATYLLTRSFAQPAWVLIALYIIATALVTLLAALPLQEYAGLAAREEH"
     gene            4072..5469
                     /locus_tag="VGT44_23485"
     CDS             4072..5469
                     /locus_tag="VGT44_23485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583837.1"
                     /translation="MATANNVPSSTMSTVIFSSTVGTAIEWYDFFLYGTMATLVFPKV
                     FFPESNQLVGTILSLFTFLVGFIARPFGGALFGHLGDRIGRKSTLIATLLLMGLSTLL
                     IGFLPGYATLGIVAALILVLLRLGQGLGVGGEWGGSVLLAMEYGHRRNRGFWASWPQM
                     GVPVGLILSTVIVNIVQSSAGTSFVTGFAWRIPFFISALLVLIGLFIRLRVLETPLFT
                     EMKNSGREVSAPIIEAFRHSTAEILLSAGARFTEQAPFYLFTTFVITYGVDKLVLGRT
                     EILNAITFAAIVELFTIPLFGYLSDRVGRRRWYLVGCVLMAAFAFPYFLLMNTGNAVV
                     FTLSVICSLAIFHAWVYGPQAALIAERFGTRSRYSGASLGYQLAAPFAGGLAPIIAVL
                     LLNGKSSLSALGLSGVVVNVGAGSWQAVSVYIVVLAVISFASVLGLKELTRADISNVD
                     AYKITEDQIVLAPTD"
BASE COUNT          995 a         1694 c         1567 g         1371 t
ORIGIN      
        1 gtggttgcgc cgcagcacgg ccttgacgcg cgccagcagc tcgccgacgc cgaatggctt
       61 ggtcaggtag tcgtccgcgc cctggtcgag cgcggcaatc ttgtcggtct cctcgcccag
      121 cgcggtaagg atgatgatcg ggacggtgct gcgctggcgg attctccggc agacctccag
      181 cccatccagg ccgggcatca tgatgtccag gataatcaac gcgaagttgg catgttcaaa
      241 gagcgccagc gcctccagcc cgttgctggc cagcgagact ttgaacgcgc gcagttctag
      301 attgcgtttg acgaaatgct gaatactcgg ctcgtcatcg acgacgagaa tgtgtttttc
      361 ctgtggcata cgctgacaca cctcttcatt gaattacagc gctttctcta gctcgaacgg
      421 cacacgccgc gaaccgggaa cgtacagcag cctgccgggc agcaagatat ggaaggtcgc
      481 cccactgtcg ttggtgttgt taccgctcct gttctccgcc cagatacgcc cgccgtgcgc
      541 ctcgacaaag ccccggcaga tcgacaggcc aagtcctacc ccgctgctgc tgcgcatcct
      601 gccgccatcg acctggtaaa accgctcgaa gatacgctcg gtcaggtctc ctggcagacc
      661 cgacccatga tccgtaacgc tcaggtagat gccctcgtct tcgtcgtcca gcgcgtagcg
      721 ggcggaaatc tctatctctg ccttgtcgcc agggtcttcc agatcggttg catattgctg
      781 cgcgttctcc aggatgttgc gcaataccat ctccatgcgg cgctgatcta tgtataacac
      841 aggtatctct tctggaagag agacatgaat gcgcaatcgt tcgcattttg ccgcgtcaac
      901 cgcgcgctgg atcagcatat ccagtcggca gtagccgggg gtcagtttga gcgttcccgc
      961 ttcgagctgt gacatctcca acaggctggt gacaagctcg gccatgcgat cttcttcctc
     1021 gacgatgcgc tgcaaaaagt cgcgctgcgt tgtcgcgtcg agttctacat cggtctccag
     1081 caggctggtt gcgtagcctt tgatggcagt cagcggggtg cgtaactcgt gcgagatgat
     1141 cgccagcagg ttctttttga tctgctcggc ttcactctgc tgcgtcacgt catgatatat
     1201 tttgccgcgc ccgatcaact ggtttgccat gtcgtagacc tggaagaggc gcatacggat
     1261 gagtcgcctg gacgggacga gctggccaag ctggttgtag aacccacgta cctggaactc
     1321 gatgacgtgt ggcccatcgc cgagttcagc gctgtgcagc gcttcgcggt aggtgtcggg
     1381 agcctccatt aaagactcca ggcgctcggt cagatgcgtt tcctggaacg agtcaagctg
     1441 caactcctgg gtggaaaagc ctgcaaattg cgttagccgg tgattggcgt agacgatttg
     1501 cccgtcggga ttttccagca ggacgccctc gtccatgctg cgcataatgg cgtccagggt
     1561 ttgcaggcgt gaaaagctct gttcgtcgct cttacggaag agcgtgtgca tctcctcttc
     1621 catcattgaa aaggcgttca ccaggcggcc aatctcgtcg tgacggctct tctccctggc
     1681 cagttgcttg ccgtccgtga ccttcgcggc ctggtccggc ctcatgatcg tggcggcctg
     1741 cgccagccgg ccaagcggcg cgaccaccca gccgtgcatc acaaaccaga agacgcttgc
     1801 cccgaccagc agcataatca gcgcgataat caggctgtgc tggaagctga tgacggctgc
     1861 aaaggtgaca tcggttgggc gaccgaccgc taccgcccag cttgtgccgg agacaggcac
     1921 gtaactatac agccagtcgc gctgttgatc ctgcgcgatg acgctgcccg gcttgctgtg
     1981 cagggcgtca cgcaggccgg gaatgcgctg caacagattg ctgtggagcg agacccccac
     2041 cgtattcgcg ataacattgc cgtcgctatc cagaatccag atgcgtactt cgccgtcggt
     2101 ggtcaactgc tgctgctcgg ctttcaaacg cgcgttcagt tgcgccaggt cgatgcttcc
     2161 tccctttgtg tcctggacct gcatctccac gttttgcgcg atggcctgcg ccaggcccag
     2221 gtcggcctgc tgcgcgctct gttgcagcgc gccatactgg aaaaaataaa gttccgcgcc
     2281 gcccagcagc agaggaatca cgaacaggcc ataaaagacc agcagttgca gggcgatttt
     2341 tcgttccagt atcctgagca ttgccagctt ctcctaagca agaggatctc attatgtata
     2401 gtatgtggta atctaacggg cattgcaatg ggtggtacac ctggaacccc gtcaatccag
     2461 aaatatttac accgttgttt acatcccggc ctcacccgtt tatagctttt atacaggtgt
     2521 caggttatgc ttagtgttag aggaagatcg atggaaacgc tccaactaga aaagactgaa
     2581 gaaacctccg cgcgggaaca actgtttccg gtcatcgtgg ccagcgcgct cggctcggct
     2641 atcgaatggt ctgatttctt ctactacagt tttcttgccg tgacggtctt tccaactgtc
     2701 ttcttttctc cgcttactcc gtcaatcggc atggtggcct cgtttagcgc gaatttcgtc
     2761 ggattcgtgg cgcggcccct gggcggcgtt ttctttggcc gctacggcga ccgtatcggg
     2821 cgcaagtcga ccctggcggc gacgctgctg ctgattggcg caaccacaat gctgatgggc
     2881 gtgctgcccg gctatgcaca gctggggatc gccgctccgc ttttgctggt ggccttgcgc
     2941 ttcctgcaag gtgttggtgt aggtggcgaa tggggcgggt ccgtgttgat gacgatggag
     3001 tttagcgacc agcgccgccg gggcttctgg acaagctggc cgcagatcgg tgtgccggtc
     3061 ggcctggcgc tttcgtcctc atcgatcctg cttttcaagg gcctctatcc cggcgaggcg
     3121 ttccaggcca tcggttggcg catcccattc ctcttgagta cgattctggt cgcggtcggc
     3181 ctctatattc gcctgcgtct ccccgaaacg cccgcgttct cgcgcctgat tgcctctcgc
     3241 cagcagtcgg tcaacatcac cctgcatacc tggcggctct actggcgcga aatcctgctg
     3301 tcggcgctga tacgcaccgg cgagcaggcg cctttttata tcttcacgac cttcgcgctc
     3361 tcgtatggca cactgatgct gcaccttagt tcgacgttga tctacaccag catgatcgct
     3421 gcttcaggcg tatcgttctg cctgatgccg tgtttcggag cgctctccga ccgcgttggg
     3481 cggcggcgca cgacgatcta cggcgcgctc gcgatgatgg tgtgcgcttt cccctttttc
     3541 ttgctgctca acaccggagt tccactgctg gtcatcctgg cgctcgcact gaccctcgga
     3601 ggacctcatg cctgcctcta tggaccgcaa tcgtccttga ttgccgagcg ctttcctacc
     3661 cgcctgcgct ataccggggc gtcgctgggg tatcaactag cctcgatcat tgcaggcggt
     3721 cccgccccga tcatcgcaac ctacctgctg acccgctcat ttgctcaacc ggcctgggtc
     3781 ttgattgccc tgtacatcat cgcgaccgcg cttgtcacgc tgctggccgc tctgcctttg
     3841 caggaatatg ccgggcttgc cgccagggaa gagcactgaa tttatttata cattctttta
     3901 catcttactt gctttgttta cgtgaaattt atccttatct tctatactct tcatgtgtgg
     3961 tttatacacc ttgcaggtaa atttgcgctt agcgagaaag gaggcagaaa cagcaatcca
     4021 ggtcttgttg tagtttcata acagcttcat tatcacttac aggaggaaca catggcgaca
     4081 gctaacaacg tcccttcaag tacaatgtca acggtgatct tttcaagcac cgttggtact
     4141 gccatcgagt ggtatgactt tttcctctac ggcacgatgg ccaccctcgt cttcccaaaa
     4201 gttttctttc ctgagtccaa ccagctcgta ggcacaatcc tgtcattgtt caccttcctc
     4261 gtcggtttca tcgcccggcc cttcggcggc gcgctcttcg ggcatctcgg cgatcgcatt
     4321 ggccgcaaat ccacgctgat cgctaccctg ctattaatgg gcctctcgac gttgttgatc
     4381 ggtttcctgc cgggctatgc cactctcggt atcgtcgcag cgctgatact tgttctcctg
     4441 cgcctgggtc agggtcttgg cgtcggtggt gaatggggcg gttctgtcct gctcgctatg
     4501 gagtatggac accggcgcaa tcgcggcttc tgggcaagct ggccgcagat gggcgtgccg
     4561 gtcgggctga tcctctcgac cgttatcgtc aacatcgtcc agtccagcgc gggtacgagc
     4621 ttcgtcaccg gctttgcgtg gcgcatcccg ttcttcatca gcgcgctact ggtactcatc
     4681 gggcttttta tccgcttgag agtcctggag acgccgctct ttaccgagat gaagaatagc
     4741 gggcgagaag tttccgcgcc gattatcgaa gcgttccggc atagcaccgc tgaaattttg
     4801 ctcagcgctg gcgcgcgatt caccgagcaa gcgcctttct acctcttcac caccttcgtg
     4861 attacctacg gtgtagacaa gttggtgctg ggtagaacgg agatactgaa cgccatcacc
     4921 ttcgccgcca tcgtggaact tttcacgatc ccgctcttcg gctacctgtc cgaccgggtt
     4981 ggcaggagga gatggtacct ggtcggctgc gtcctgatgg cggcctttgc tttcccctac
     5041 ttcttgctaa tgaataccgg aaatgcggtt gtcttcaccc tgtcggtcat ctgctcgctt
     5101 gccatcttcc acgcctgggt ctacggcccg caggctgcgt tgatcgccga gcgcttcggg
     5161 acgcgctccc gttacagcgg cgcgtcgctg ggctaccagc ttgccgcgcc gtttgccggt
     5221 ggcctggccc ccatcatcgc cgttctgctg ctcaatggca aaagcagcct ctccgcgctt
     5281 ggcctgagcg gtgtcgtggt caacgttggc gccggttcct ggcaggccgt atcggtctac
     5341 atcgtcgtgc tggcggttat ctcgttcgcc tcggtgttgg gtctgaagga attgacgcgc
     5401 gcagacatct cgaatgttga tgcgtacaag atcaccgaag accagattgt gctggctccc
     5461 accgactgat cgcgcgtatt gcagctacac agtaatgccg ccatatcgct aatctatacc
     5521 ggttggcgat atggcggcgt ttgaacccga tcaggctcga ctaaaatgcg taagccttac
     5581 tattgatgac gacgttatca accaggacaa cctgttgtcc atcggta
//
LOCUS       DASZAK010001341         3482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_566353, whole genome shotgun sequence.
ACCESSION   DASZAK010001341 DASZAK010000000
VERSION     DASZAK010001341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566353"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..262
                     /locus_tag="VGT44_23490"
     CDS             32..262
                     /locus_tag="VGT44_23490"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HEV2583838.1"
                     /translation="MKNQRITLPVYDLSCGGGGVLIIERALARTPGVVYVYVNPATEM
                     AYVEYDPYLYDPDRLVKVVEHAGFRAGVPSLR"
     gene            291..1391
                     /locus_tag="VGT44_23495"
     CDS             291..1391
                     /locus_tag="VGT44_23495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583839.1"
                     /translation="MSLAYRNLLQDKLRSTLSIVGVGLAIMLVLILNGFLVGVDRAAS
                     TYLDHAPGSVVVARKDVKNFIVSSSLLAPGVADTVQKVEGVAKVAPILTQPVYFELHG
                     QKQFVEMIGYDSRFGGGPWNLDKGREPRTDTEMVLDRTLAQQHGIVPGATLPVLNRTF
                     TVVGLSTGTSTWMTSFAFVRKTAAESLLLAPGATGFLLVTPANGISAETVRDRLSTLP
                     GTQVLLKSELVANDVALLTRSFSAPLRLMVTIALLVGVLVVGLIIYTATVERQREYGV
                     LKAIGARNRRLYRVVTTQAILVATVGSMIGIGLALGGAQLIMALRPQFLVVIEPMASL
                     QALAAGFVIAGLSALFPARLLARLAPADVFRR"
     gene            1468..2628
                     /locus_tag="VGT44_23500"
     CDS             1468..2628
                     /locus_tag="VGT44_23500"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583840.1"
                     /translation="MFRYALRNLLQNKIRLVISVGGVALALSLILALDAIMTGVEGRL
                     TAYIDHSGADIFVSQAGVRNLHMVSSWLPASIADKVQAVPGVAAVTPIMYLTDTMDVG
                     PKEDRYSIYVIGLPQGATMGGPWSIARGVSIPADGQAVIDRSVAEQYGLGIGDKVNIL
                     GQELTIAGLSDGTGNLLNSVAFISLNDFARVRGNMAMGNAASTNATMPIISFVLVKVT
                     PGASPDAVAARIEQEVPGVTAQSREAFATQERKIVKDMATDLITTMNLVGFLVGLAVM
                     ALTVYIAALSRRGEYGILKAVGARNRYLYGTVLAQALYSVVVGFALALTITFLLSVAL
                     PNLASTLTLQISSASLLKVGIASLVIAALSALLPIRQIAGLDPATVFKGART"
     gene            2625..3464
                     /locus_tag="VGT44_23505"
     CDS             2625..3464
                     /locus_tag="VGT44_23505"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583841.1"
                     /translation="MTNKATLHIAHVSKHYGSGTALISAVRDVSLEVAPGEIVLMMGP
                     SGSGKTTLLQMMGALLKPTEGTIQLNGTVLSSLAEHRLPGIRLHQFGFIFQDFNLLSA
                     LTVLDNVALVAELASTKRSEARKRAAALLTELGLGERLHFLPEQLSGGEKQRVSIARA
                     LVNDPGLILADEPTANLDSKIGHEIMRLLQMIAKEQGRSVVIVSHDQRIQDIADRVLW
                     LEDGQFKEMATMATDPVCNMKIEREKAVTTEWEGQPYYFCSRGCRREFLEGPHQFLNT
                     IPQ"
BASE COUNT          736 a          958 c         1020 g          768 t
ORIGIN      
        1 gctctgctgc aatgacagga gggagaacaa gatgaagaac caacgtatta cattgcccgt
       61 gtacgacttg agctgcgggg gaggaggggt gctcatcatc gaacgtgcgc tggcccggac
      121 gccaggagtg gtgtatgtct acgtcaatcc tgcgacggag atggcgtatg tcgagtatga
      181 tccctacctt tatgatcccg atcgcctggt gaaagtcgtt gaacatgccg gcttccgcgc
      241 aggcgttccg agcttgaggt gaggtaggta ttgaaggaag gagtgggaag atgtctcttg
      301 cttatcgtaa tctcttacaa gataagctgc gttccacgct cagtatcgtg ggcgtgggac
      361 tggccattat gctcgttctg atcctgaacg gctttctggt cggagtagat cgcgcagcct
      421 caacctacct cgatcatgcc cctggctcgg tggtcgtcgc ccgcaaagac gtgaaaaatt
      481 tcattgtctc cagttcgctc ctggcgcctg gtgtggctga cacagttcaa aaagttgagg
      541 gagttgcaaa ggtggctccc atcctcacgc agccagtcta ctttgagttg cacggacaga
      601 agcaatttgt cgagatgatt ggctatgact ccaggtttgg aggcggaccg tggaacctgg
      661 ataagggacg cgaacctcga acagatactg aaatggtgct ggacaggacg ctggcacagc
      721 agcatggaat tgtgcctgga gccacacttc ctgtgttgaa ccgtaccttc actgtggtcg
      781 ggctctccac aggcacgagc acctggatga cgagcttcgc gtttgtccgt aaaacagcag
      841 cggagtcgct cctgctggca cccggcgcta ccggctttct gctcgtcacc ccagcgaatg
      901 gaatctccgc cgagacggta cgcgatcggt tgagcacgct gcctggcaca caagtcttgc
      961 tcaagagtga gctcgttgcc aacgatgtgg ccctgctcac ccgctccttc agcgcacccc
     1021 ttcgcttaat ggtgacgatt gccctgctcg tcggcgtgct cgtcgtcggg ttgatcatct
     1081 acacagcgac cgtcgaacga cagcgcgagt acggtgtgct caaagcgatc ggcgctcgta
     1141 acaggagact ctatcgtgtc gtgaccacgc aggcaatact tgttgcaacc gtaggatcca
     1201 tgatcggcat cggactggct ttaggcggag cgcaactgat catggcattg cgtccgcagt
     1261 ttctggtagt gatcgaacca atggcctccc tgcaggcgct ggctgctgga ttcgtcatag
     1321 cggggctttc agcgctgttc cctgctcgcc tgctcgccag gcttgccccg gcagacgttt
     1381 ttcggaggta gtcgattcat ccctgctgag tgcgcagcac tagctgcggc agcagcgttg
     1441 cagggatgga tttcaaggag agagacgatg ttccgttatg ccttacgcaa tctcttgcaa
     1501 aacaaaattc gactggtcat ttccgtcggt ggagtcgccc tggcactctc cctgatcctt
     1561 gcgcttgatg cgatcatgac aggtgtggag ggacggctga ctgcctatat cgatcactct
     1621 ggggcggaca ttttcgtttc gcaagccggg gtacgaaacc tgcacatggt ctcctcctgg
     1681 cttcccgcct cgattgcaga caaggtgcag gcggtccctg gggtggcagc ggtcactccc
     1741 atcatgtacc tgaccgacac catggatgtg ggtcccaaag aggaccggta ttccatctat
     1801 gtaattggcc tgccacaagg agccaccatg ggagggccgt ggagtatcgc ccggggggtc
     1861 tccatcccgg cagatggaca agcggtcatc gatcggagtg tggcagagca gtatggcctt
     1921 gggattgggg acaaggtgaa catcctgggc caggaactca ccattgctgg cctttctgac
     1981 ggcaccggca atttgctcaa ctcggtggcc ttcatctccc tgaacgactt tgcacgcgta
     2041 cgcggaaaca tggcgatggg caacgctgca tccaccaacg caacgatgcc aatcatcagc
     2101 tttgtgctgg tgaaagtgac accgggagcc tcgcctgacg cggtcgcagc gcggatcgaa
     2161 caagaggtgc cgggtgtgac agcccaatca cgggaagcat ttgccaccca ggaacggaaa
     2221 atcgtcaaag acatggcgac tgacttaatc acgaccatga acctggtggg attcctcgtc
     2281 gggttagcgg tgatggcgct cacggtctat attgccgccc tctcccgacg aggagagtat
     2341 ggcatcctga aagcagtggg cgcccgcaat cgctacctct atggcacggt cttagcacag
     2401 gcattgtata gtgtagtggt aggatttgcg ctggcactga ctatcacttt cctgctctcg
     2461 gtggctctgc cgaaccttgc atccaccctc acgctgcaaa tcagtagcgc atcattactc
     2521 aaagttggta tagcgtcgct ggtgattgcg gcactttccg cacttctccc catccgacag
     2581 atcgccggac ttgacccggc caccgtcttc aaaggagcac gaacatgacc aataaggcaa
     2641 cattacacat cgcacatgtc agcaagcact atgggtcagg taccgccctc atctcggccg
     2701 tccgtgatgt ttcgctcgag gtggcgccgg gtgagatcgt cctgatgatg gggccatctg
     2761 gttctgggaa aacaaccctg ctccagatga tgggagcctt gcttaaaccg acagagggaa
     2821 cgattcaatt gaacggaacg gtgctcagct cattggcgga acatcgcctg cccggaatcc
     2881 gcttgcatca attcgggttt atcttccagg atttcaacct gctctcagca ctcacggtcc
     2941 tggacaatgt cgctctcgtg gcggaactcg ccagtacgaa gcggagtgag gccaggaaaa
     3001 gggctgccgc gctcctcacc gaactcgggc tgggcgagcg gctgcatttc ctcccagaac
     3061 aactgtctgg tggtgaaaag cagcgggtat ctattgctcg tgccctggtg aatgaccctg
     3121 gcctgattct ggctgacgag ccgacggcga atctagactc gaagatcggg cacgagatca
     3181 tgcggctact acaaatgatc gcgaaagaac agggtcgcag tgtcgtgatt gtctcccatg
     3241 atcaacgcat ccaggatatc gccgaccgtg tgctgtggct tgaggatgga cagttcaagg
     3301 agatggcgac catggcgacc gatccagttt gtaacatgaa aattgagcgt gagaaagcgg
     3361 tcacgacgga gtgggagggg cagccctact acttctgctc gcggggctgc cgcagggagt
     3421 ttttagaggg gcctcaccag ttcttgaata ccattcctca atagaagagc gaaatccacg
     3481 ct
//
LOCUS       DASZAK010001342         3235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_69182, whole genome shotgun sequence.
ACCESSION   DASZAK010001342 DASZAK010000000
VERSION     DASZAK010001342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69182"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /gene="hemW"
                     /locus_tag="VGT44_23510"
     CDS             <1..1344
                     /gene="hemW"
                     /locus_tag="VGT44_23510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEV2583842.1"
                     /translation="GGSGGEWGGDPCGRPGTHLLHVIDDAESGGLYSGTYAPAGTFTH
                     MHTHTFTTETDIQQLLATAALYLHIPFCHTRCHYCDFNTYAGILPMREPYVRALLREI
                     ALAGVMARKDDGEPRRSRTIFFGGGTPSLLTVAQVARLLDACRASFALDEDGEITLEA
                     NPGTLDQGQLSGLRAAGVNRLSLGSQSFDAELLKTLGRIHSPDEIEQALRFARSAGFT
                     SVNLDFMFGLPGQTMRHWQNTLDRALFLRPEHFSLYSLIIEEGTPFYTWVQQGHITPG
                     DEDLCADMYEYADERLQNEGYVNYEISNWALPGHESRHNLTYWRNLPYLGLGAGAHSF
                     FAGKRFSNERDPQRYIDLLKQQRLPIVESETVGRAQEMSETAFLALRTAQGLHLPTFE
                     ERFATSFASFAGDRLRPVEAAGLLEYADDGWLRLSKRGRLLGNEVFMRLLPDDEC"
     gene            1353..3170
                     /gene="recJ"
                     /locus_tag="VGT44_23515"
     CDS             1353..3170
                     /gene="recJ"
                     /locus_tag="VGT44_23515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV2583843.1"
                     /translation="MVDQSSVSTWTTCPQLSRAQFDEYRKVNIEALHAQLLYNRGITT
                     PDNMQRFLAARFDQLYDPFTLNDMGKAVERITRALQGSEHITVYGDFDADGVTSAALL
                     SRALRGLLRPGASLDYFIPHRLEHTRGLSRETLDSISSRGTQLLITTDCGSSDGTEID
                     YAKTLGIDVIITDHHQPPPDLPPAYAVINPWRHDNLYPEQYLCGAGIAFKLAQALYRA
                     YEREEEVKALLDLVAIGTISDVAPMLGENHTLVRLGLEQLNATTNAGLSALIELAGLQ
                     TRRLRERDISYALGPRINAAGRMKDAGIAFELLTTGNETEARTRARELEELNLSRQQI
                     TDELMKRVREQAQEQAGNAVILVYGDKSEWPEGIIGLVAGRLSEEIRRPVFVLSQDSE
                     TSRGSARSQSDFNIIEALRQRADLFERHGGHAQAAGFTIANANIEELRRHLLAWHTTS
                     NGHGNPDEAPPILEAGISPELTVVSEPDTPTETRKVDLKIGKPETQITYDAYMKASQL
                     APFGTGNPEPAFRLDSALIIRRWLSGPEGRHLRLRLRVTGAQYNGTFLRAGSRIDAFP
                     EGARVNIIFCLEPQWNAPENDERREVWLKIMAMEPASAV"
BASE COUNT          697 a         1068 c          901 g          569 t
ORIGIN      
        1 ggcgggagcg gcggggagtg gggtggagac ccttgcggtc gccctggaac tcacttatta
       61 catgtgatcg atgatgccga atccggtggt ttatactctg ggacttacgc cccagcgggt
      121 acctttactc atatgcacac acacacgttc acaaccgaga cggacattca gcagctctta
      181 gcaacagctg cgctctatct ccatatcccg ttttgccata cacgctgtca ctattgcgac
      241 ttcaacacct acgcgggtat cctcccaatg cgcgaaccct acgtgcgcgc gttgctgcgc
      301 gaaatagcgc tcgctggtgt tatggcgcgc aaggacgatg gcgagccgcg ccggtcgcgc
      361 accatctttt tcgggggcgg cactcccagc ctgctcaccg ttgcccaggt cgcgcgcctg
      421 ctcgatgcct gccgcgcctc cttcgcgctt gacgaggatg gcgagatcac gctggaagcc
      481 aaccccggca cgcttgacca ggggcagttg agcgggctgc gcgccgccgg tgtgaaccgc
      541 ctcagcctcg gctcgcaaag cttcgacgcg gagctgctca aaacgctggg gcgcatccac
      601 tcccctgacg agatcgaaca ggcgctgcgc ttcgcgcgct cggcaggctt cacctccgtc
      661 aacctggatt tcatgtttgg tctgccgggc cagaccatgc gccactggca aaacaccctt
      721 gaccgcgccc tgtttctgcg ccccgaacac ttctcgctct actcgcttat catcgaagaa
      781 ggcacaccct tctacacctg ggtacagcaa ggacacatca cccccggcga cgaagacctg
      841 tgcgccgata tgtacgaata tgccgacgaa cggctgcaaa acgagggata cgtcaattac
      901 gaaatctcga actgggcgct gccaggacac gagagccgtc acaacctgac ctactggcgc
      961 aacctgccct acctgggcct cggagcgggc gcgcattcct tctttgcggg caaacgcttt
     1021 tccaatgaac gcgacccgca gcgctacatc gatctgctga aacagcagcg cctgcccatc
     1081 gtcgaaagcg agacagtcgg acgcgcgcag gagatgtcgg agacagcctt ccttgccctg
     1141 cgcactgccc agggcctgca cctgcccacc tttgaagagc gtttcgccac ctccttcgcc
     1201 tctttcgccg gagaccgcct gcgcccggta gaagccgccg gactgctgga atacgccgac
     1261 gacgggtggc tgcgcctcag caaacgaggc cgtctgctgg gcaacgaagt attcatgcga
     1321 ttattacccg atgatgaatg ttgaggcaca ttatggtcga tcaatcatct gtatcaacct
     1381 ggaccacctg tcctcaactc tcccgcgcgc agtttgatga atatcgaaaa gtaaatatag
     1441 aggcactgca cgcacaactg ctttataatc ggggcatcac cacgcccgac aacatgcagc
     1501 gcttcctggc agcgcgcttc gaccagctct acgatccttt cacgttgaac gatatgggga
     1561 aggcggtcga gcgtatcaca cgcgctttgc aaggcagcga acatatcacc gtgtacggcg
     1621 atttcgacgc cgacggcgtg acctccgccg cgctgctttc gcgcgccctg cgcggcctgc
     1681 tgcggcccgg cgcgtcgctc gactacttca ttccacaccg cctggaacat acgcgcggct
     1741 tgagccggga aacgctggac agcatttctt cccggggaac gcaactgctc atcacgaccg
     1801 attgcggtag ctcggatggc accgagatcg actacgcgaa gaccctcggc atcgatgtca
     1861 tcattaccga tcatcaccag ccaccgccag acttgccgcc tgcctacgcc gttatcaacc
     1921 cctggcggca cgacaatctc tacccggagc aatacctgtg cggcgccggt atcgccttca
     1981 aactggcaca ggcgctctat agggcctacg aacgcgaaga agaagtaaag gcactgctcg
     2041 acctggtagc gattggcacc atcagcgacg tagcccccat gctgggcgaa aatcatacgc
     2101 tggttcgcct gggtctggag caactcaacg ccacaaccaa tgctggtttg agcgccttga
     2161 tcgagctggc cgggctgcaa acaagacgcc tgcgcgagcg cgatatctct tacgccctgg
     2221 ggccgcgcat caacgccgcc gggcgcatga aagacgccgg tatcgccttt gaactgttga
     2281 cgaccggaaa cgaaacagaa gcgcggacca gagccagaga actcgaagaa ctcaacctgt
     2341 cgcgccagca gatcaccgac gaactgatga aacgggtgcg tgagcaggcg caggagcagg
     2401 ctggtaatgc cgtcatcctg gtctatggcg acaaaagcga atggccggag ggcattatcg
     2461 ggctggtggc cggtcgcctc tcggaagaaa tccgccgccc ggtcttcgtt ctcagccagg
     2521 atagtgagac cagccggggt tcggcacgca gccagagcga tttcaacatc atcgaagcgc
     2581 tgcgccagcg agccgacctg ttcgagcgac acggcggcca cgcccaggcg gcaggcttca
     2641 ccatcgccaa tgccaatatc gaggagttgc gcagacacct gctcgcatgg cacaccacca
     2701 gcaacggtca cggcaatcca gacgaagcgc cacccatcct cgaagcaggc atctcgcccg
     2761 aactaaccgt cgttagcgag ccggatactc ccacagagac gcgcaaagta gacctcaaga
     2821 tcggcaagcc agagacgcag atcacctatg atgcctatat gaaagccagc cagcttgccc
     2881 cctttggcac gggcaacccc gaacccgcct tccgactcga cagcgcgctc atcatccggc
     2941 gctggctgag cggccccgaa gggcgacacc tgcgcctgcg cctgcgcgtc accggcgcac
     3001 agtacaacgg caccttcttg cgcgccggtt cgcgcatcga cgccttccca gaaggggcgc
     3061 gtgtcaacat tatcttctgc cttgagccac aatggaacgc ccctgagaac gacgaacgcc
     3121 gcgaagtctg gctcaagatc atggcgatgg aacccgcaag cgcggtataa ttacgaggaa
     3181 gtaccgcatc aaggggtcca gaagggcgac cgcaagggta cgcccttaca atgac
//
LOCUS       DASZAK010001343         1790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_210376, whole genome shotgun sequence.
ACCESSION   DASZAK010001343 DASZAK010000000
VERSION     DASZAK010001343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210376"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1362
                     /locus_tag="VGT44_23520"
     CDS             172..1362
                     /locus_tag="VGT44_23520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2583844.1"
                     /translation="MPWYRASRTILPIYLAFHLAFFLITCFAVLFVLKDFSSQTLNID
                     NWLHSWSHWDANQYDYIALRGYEQWWKTVFFPLYPLLQRCLMIFTHDPLFAGLIISNV
                     AGLGMLTVLYRLVEEDFDAERAYRAVLYLSVFPTAFFFAAAYTESLFLFLALLSFYNM
                     RHGHWWFAGLLGLLATLTRQAGLFLLVPFYCEYLRQRGDFKDVWSSTGNGIERLLRSL
                     LRFDFFAGALILVGMGIFVVYCYYRFHILFPFSRAEGLYWNRDLHDPSWGIVRTIQVI
                     AHSPGFLSFSALRNMLDLGQDLFIFVLIVISLLGPWRFRGAVWTYAVYGVTLYLCLQL
                     YPVRDFSFPLQSFPRYMLEVFPAFIVLAGIDQHRRINLVYLVASASLLFLLLTQYVTG
                     HWIT"
     gene            complement(1379..>1790)
                     /locus_tag="VGT44_23525"
     CDS             complement(1379..>1790)
                     /locus_tag="VGT44_23525"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV2583845.1"
                     /translation="NLGSAAFLSAGGYHHHIGLNTWESKDGPPPAPGTTGLYHFAINY
                     PARKDLATALKRLLDHGWSIDGASDHGTHEAIYLHDPDFNGIELAWDRHPSQWPRGDG
                     ALTFSRQPLDFASLLAELEEPDEAGYLKVLSAYK"
BASE COUNT          341 a          526 c          468 g          455 t
ORIGIN      
        1 aacaaacttg atatcaacca aaccgactat agccccgcgg gtgacaccga cagtgatgaa
       61 gagacggcca agatagctgc tttacgagga aacagcacta acccttcagg tgaaagaacc
      121 tccaccacag aaacaggcaa caacagaaac cagcgtataa aatcgttctg gatgccctgg
      181 tacagggcat ccagaacgat tttaccaatc taccttgcct tccacctggc atttttctta
      241 attacgtgtt ttgccgtttt attcgtcctc aaagatttct catcccagac tctgaacatc
      301 gacaactggt tgcattcatg gtcccactgg gatgctaacc aatacgacta catcgcactc
      361 cgtggctacg agcagtggtg gaaaaccgtc ttctttccct tatacccctt gctgcagcga
      421 tgtctgatga tcttcacaca tgacccgctc ttcgcaggcc tcattatctc caatgtcgcc
      481 ggtctgggca tgctcactgt gctctatcgc ctggtcgagg aagatttcga tgccgagcgt
      541 gcgtaccgcg cggtgctcta tctctccgtg tttcccacgg ctttcttctt cgccgctgcg
      601 tataccgagt cgctctttct ctttctggcc ctgttgagtt tttacaatat gcggcatggt
      661 cactggtggt ttgccggtct gctcggttta ctggcaaccc tgacacgcca ggcaggattg
      721 ttcctcctgg tccccttcta ttgtgagtat ctgcgccagc gtggcgactt caaagatgta
      781 tggtcgagta ccggcaatgg cattgagcga ctcctacgct cactgctgcg cttcgatttc
      841 ttcgccggag cactcatcct ggtaggcatg ggcatctttg tggtctattg ctattaccgc
      901 tttcacatcc tcttcccctt ctcacgcgcg gaaggattgt attggaaccg cgacctgcac
      961 gatccttcgt ggggcatcgt ccgtacgatc caggtcatcg cacatagccc aggatttctc
     1021 agcttcagcg cactgcgtaa catgctggac ctggggcagg acctgttcat atttgtgctg
     1081 atcgtgatca gcctcctcgg tccctggagg ttccgcgggg cagtttggac atatgctgtc
     1141 tatggagtca ccctatacct gtgcctgcag ctctaccccg tgcgagattt ctctttccca
     1201 ttacaatcgt ttccgcgcta tatgctagag gtctttccgg ccttcatcgt gctggccggg
     1261 atcgaccagc accgtcgaat caacctggtc tacctggttg cctcggcttc cctgctcttc
     1321 ctgttgctga cgcagtacgt gacggggcat tggataacct gaaagaaaaa gtctttccct
     1381 acttgtacgc cgacaaaact ttcaagtatc ccgcctcgtc cggctcctcg agttcggcga
     1441 gtaggctcgc gaagtcgagg ggctgccggc tgaacgtgag agctccgtcc ccgcgcggcc
     1501 actggctggg gtgccggtcc caggccaact cgatcccgtt gaaatccgga tcgtgcaggt
     1561 agatggcctc gtgggtgcca tgatcggacg ccccgtcgat cgaccagcca tgatcgagca
     1621 agcgtttgag cgcggtggcc agatctttgc gcgccggata gttgatggcg aagtggtaca
     1681 ggccggtcgt gccgggagcg ggaggtggcc cgtccttgct ctcccaggtg ttgagaccaa
     1741 tgtgatggtg gtagccgcca gcggagagga aggctgcgct ccccaggttg
//
LOCUS       DASZAK010001344         3162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_210381, whole genome shotgun sequence.
ACCESSION   DASZAK010001344 DASZAK010000000
VERSION     DASZAK010001344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210381"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2153
                     /locus_tag="VGT44_23530"
     CDS             <1..2153
                     /locus_tag="VGT44_23530"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEV2583846.1"
                     /translation="RNYRSRPDIVAIAESFRHSRLEPDADAIAAESARPGHADAYITL
                     ASAPDQASELHGLVEDMRHKHTQGYAYRDIVALCRTRALARTVTRALVEAGLPVIERG
                     GMLEQVHIRALLSTIMLLSDSGSMGILRAARQPDHPFSQADIEALLAATREEHSGETH
                     ISLRQIFLSGHAPPGLSREGQRSFAKLSAILKSMYFPPAITSIWQLLAQYLFIESSIG
                     RQLLSSDVVGTETGALRADYADLLALARFFDAQALRQREQREEAAGLLDTPGATDAPP
                     IAEQVRAFLDYLAVLLTLRQDGGGSRREAEEQDGATPDVIRVMTVHASKGLEFPVVYL
                     PGVVNQKFPAQRRWQPTPPPRGMIAAGDDEAAAHETGEACLFYVAATRARDYLIISFA
                     ERYSKRVSKPSSYIHALAADLSDERIARVAWPKINREDTQSSQAGDADTAEDQPDGSL
                     SSQPGEAFLAASTPSTLRISAIETYQRCPRQYLYGSIYRFRGEAAAYQLFRRATQQTL
                     DGLQKRIEAAGASDYSEQGERVEARYPTTDEARELYSQHWRAADGHTTPFAPLYEQHG
                     HEVAELLRRKLLASGSSGWQLSPTYTVEVAGQPIEVTIDRVESPASGGAVEPLRFVKT
                     GYGRRKTKVDPTARELLYARAYRQHHPDRPIALHFHNMSTGETFEIKLTQKKEQSLYQ
                     ELEKALGGLARHEYPPKPDMQVCPGCSFFLICPA"
     gene            complement(2202..2591)
                     /locus_tag="VGT44_23535"
     CDS             complement(2202..2591)
                     /locus_tag="VGT44_23535"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEV2583847.1"
                     /translation="MAIDKDASSARRSFYRRPHQVDRLINQFLDAACDPSRRSILELL
                     IPPDGEETPTAYELRAGEIARQIGLAPSTTSEHLHHLMNLHLVSSRKEGNIVYYCLRN
                     RHLIRAFHELLQALSTHYATLDVASEG"
     gene            complement(2746..>3162)
                     /locus_tag="VGT44_23540"
     CDS             complement(2746..>3162)
                     /locus_tag="VGT44_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2583848.1"
                     /translation="MSIEMPETPSSQEKSLQIILVVEDDEAIGDLLVQILKSETPYVT
                     LLVTDAMQALETVKSIKPGLFILDYHLPGINGVELHDRLHAIKGLETVPTLMMSARAP
                     ARQAIGQRHITLLRKPFELATILKAISSLLDPQEES"
BASE COUNT          656 a          982 c          893 g          631 t
ORIGIN      
        1 gccgcaacta tcgctcgcgc cccgatatcg tcgccatcgc cgaatcattt cgtcattcgc
       61 gcctggaacc cgacgccgat gcgatcgcag ccgaaagcgc gcgccccggc catgccgatg
      121 cctatatcac actggcgagc gcacccgatc aagccagcga actgcacggc ctcgtcgaag
      181 atatgcgtca caaacacacg cagggctacg cgtatcgcga tatcgtggcg ctgtgccgca
      241 cgcgcgccct cgctcgcacg gtcacgcgcg ccctggtcga agctggcctg cccgtcatcg
      301 agcgcggcgg gatgctcgag caggtacata ttcgcgcctt gctctcgacc atcatgctgc
      361 tcagcgactc aggcagcatg ggtatcctgc gcgccgctcg ccagcccgat cacccctttt
      421 ctcaggccga tatcgaagcc ctgctggcgg ccacccgcga agagcattca ggtgaaacac
      481 acatttcgct ccggcaaatc ttcctgagcg gacacgctcc gccagggttg agccgcgagg
      541 gacagcgctc cttcgcgaag ctttccgcca tcctcaaatc aatgtatttc ccccctgcca
      601 ttactagcat ctggcagttg ctcgcgcaat acctctttat cgagtcctcg ataggccgcc
      661 agttgctctc cagcgatgtc gtagggacgg agaccggtgc gctgcgcgcg gattacgccg
      721 atttgctggc actggcgcgc tttttcgacg cgcaggcgct tcgccagcgt gagcagcgcg
      781 aggaggcagc cggtctgctt gacacaccag gcgcaactga cgccccaccc atcgcggagc
      841 aggtgagggc gttcctggat tacctggccg tgctgctgac gctgcgccag gatggcggcg
      901 gcagccggcg cgaggccgag gagcaggacg gcgcgacgcc cgatgtgatt cgtgtcatga
      961 ccgtccacgc cagcaagggc ctggagtttc cggtcgtcta tttgccaggc gtcgtcaacc
     1021 agaagtttcc ggcgcagcgg cgctggcagc caacgccccc accccgcggc atgatcgcgg
     1081 ctggcgacga cgaggccgcc gcgcacgaga ccggcgaagc atgcctcttc tacgttgccg
     1141 ccacgcgcgc gcgcgattat ctcatcatca gtttcgccga acgctacagt aaacgagtat
     1201 cgaagccttc gagctatatc catgccctgg ccgccgatct ctccgatgag cggattgcgc
     1261 gggtggcctg gccgaagatc aaccgcgagg atacacagtc gtcgcaagct ggagatgccg
     1321 atacagcaga agaccagccg gatgggtcgc tttcgtcgca gccgggcgag gcttttctcg
     1381 cggccagcac accctccacg ctgcgcatct cggccattga gacctaccag cgctgcccgc
     1441 gccagtacct gtatggctcg atctaccgct tccgtggcga ggccgccgcc tatcagctat
     1501 tccgcagagc cacgcagcag acgctcgacg ggctgcaaaa gcgtatcgag gcggctggcg
     1561 caagcgatta tagcgaacag ggcgagcgtg tagaggcgcg ctacccaacg acagacgagg
     1621 cgcgcgaact ctattcgcag cactggcgtg ccgccgatgg ccatacgacg cccttcgcgc
     1681 ccctatacga gcagcacggc cacgaagtcg ccgaactgct gcggcgcaag ctgctcgcaa
     1741 gcggcagctc cggctggcaa ctctcgccga cctatacggt tgaggtagcc ggtcagccca
     1801 tcgaggtgac gattgaccgc gtcgaatcgc ccgcgtccgg cggcgcggtc gaaccactca
     1861 ggttcgtcaa aactggctac ggacggcgca agaccaaagt cgatccgacc gcgcgcgaac
     1921 tgctttacgc acgcgcctac cgccagcatc atcccgaccg gcccatcgcg ctgcacttcc
     1981 acaatatgag taccggcgaa accttcgaga tcaagctaac gcaaaagaaa gagcagagcc
     2041 tctaccagga acttgaaaaa gctctgggcg gcctggcgcg tcatgaatac ccgccaaagc
     2101 ccgatatgca ggtatgtccg gggtgctcgt tcttcttgat ttgcccggcg taacaggggg
     2161 ctgttggtct ggggttcgag tccctggtcg cccacttctc ttcacccctc cgaagcgaca
     2221 tccagagttg catagtgggt actgagcgct tgaagcagct cgtgaaaggc gcgaatgaga
     2281 tggcgattgc gcaggcaata atagacgata ttgccttctt tacgagaact gaccaggtgc
     2341 aggttcatta gatggtgcag gtgttccgag gttgtggatg gcgccaggcc gatctgccga
     2401 gcaatctccc ctgcccggag ttcatatgca gtgggggttt cctcgccatc gggtgggata
     2461 agcagttcca ggatagagcg ccggctcgga tcgcaggcgg catctaaaaa ttgatttatc
     2521 aagcgatcga cctgatgagg ccgacgataa aaactccttc ttgctgaaga ggcatccttg
     2581 tcaattgcca taacaataca ctactttctc cgtacaggac tctatgtttg tcgtttactt
     2641 catattcctt ttacgacccc gctgagtttt ttgttacagt atgattcacc gcgaacacct
     2701 gccccgcttc acttatgaag aaagataaac tttgcgtagc gcaggttacg attcttcctg
     2761 cggatcaagc agcgatgata tggccttcaa aatggtcgct agctcaaatg gttttctgag
     2821 gagggtgata tgacgctgtc ctatggcctg acgagcagga gcacgagcgc tcatcatcag
     2881 cgtaggcact gtttccagcc ctttgatggc atgtaagcgg tcatgcagtt ccaccccatt
     2941 gatgccaggc aaatgataat ccagaataaa taagcccggt ttgatagact tgactgtttc
     3001 taaagcctgc attgcatctg taacaagcag tgtgacatag ggcgtttcgc tcttaagaat
     3061 ctgcacaagc aaatcgccga tcgcttcgtc atcctcaaca actaaaataa tttgaaggga
     3121 cttctcctgg ctggaaggag tttccggcat ttcgattgac at
//
LOCUS       DASZAK010001345         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_246999, whole genome shotgun sequence.
ACCESSION   DASZAK010001345 DASZAK010000000
VERSION     DASZAK010001345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246999"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="VGT44_23545"
     CDS             <1..806
                     /locus_tag="VGT44_23545"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV2583849.1"
                     /translation="FTTLGVPILAGRDFADTDNAKTPRVGIINEEFAARFLPNVNPLG
                     HIIGSDDGRFSMTIVGVVKNHKYRSIDETPIPMAWWMYAQIPVVGEMHVEMRVNGAPL
                     AILPSAQKVVQQLDPNLPLIKPMTQRAQYDTTISSQIMFARLAEFFGLLAVVLVATGL
                     YGTLSYRVNRRTAEIGVRMAMGAGRGQVVWMILRDSLRLTVIGVVAGVPLAMLIGRAL
                     SSSLYGVKPLDALTYAFAIAGVAVVALFASAAPASRAASVDPLKALRTE"
     gene            complement(810..1505)
                     /locus_tag="VGT44_23550"
     CDS             complement(810..1505)
                     /locus_tag="VGT44_23550"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEV2583850.1"
                     /translation="MKSNIAIANTRAFESARRVNRSLTAGMERRALTWMAERAPGWVT
                     SDGLTLLGLLAQIGAGVFYALSRYNDAGLVGVIVCIVMNWFGDSMDGTLARVRNQQRP
                     RYGFYVDHMVDMLGATAMMCGLGLSGFVHWQVALAVLIAFLLLSAESYLATYTLSCFQ
                     MSQGIFGPTEIRILLIAGNVALLRSPYATVLGHRWLLFDVGGAIAAAAMLAMAIAVGV
                     RHTAELYRQEPLS"
     gene            complement(1530..>2384)
                     /locus_tag="VGT44_23555"
     CDS             complement(1530..>2384)
                     /locus_tag="VGT44_23555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583851.1"
                     /translation="GVETGNLGDMKRRIEGRRTGFQAVGLLIWAMILTPWLAGEAPAT
                     ATAAFNAYAAKLEAGLGEQHRSGNGFVATVGFNGPEDSRLRKGELIIEKLTPTGEADL
                     RGAMLHDWRGTAFIRGASVADFERLMKNYAAYPQRFSPQVMEAKILPEQVGDPNHFMV
                     LMRVRQHHVITVVMDTTYDVTFRRLDAQHAFSISRSTRISEIGSPGTAKEHALSGSEE
                     HGFLWRLNTYWSCEERDGGIYVQIESASLTRSIPAGLGWVIRPFVESVPRESLEFTLH
                     AVCDALRR"
BASE COUNT          468 a          829 c          626 g          461 t
ORIGIN      
        1 tcttcaccac tctcggtgta cccatcctcg caggccgcga cttcgccgat actgacaacg
       61 cgaagacgcc ccgcgtcggc atcatcaatg aggaatttgc agcgcgtttt ctccccaatg
      121 tgaatccgct cggccacatc atcggatccg atgacggacg cttcagcatg accattgtcg
      181 gcgtggtgaa aaatcacaag taccgcagca tcgacgaaac gcctatcccc atggcctggt
      241 ggatgtacgc gcagattccc gtcgtcggag agatgcacgt ggaaatgcgc gtaaacggcg
      301 cgccgctcgc cattcttcca tctgcgcaaa aggtcgtcca gcagcttgac ccgaacctgc
      361 cgctcatcaa gcctatgacg cagcgtgcgc aatacgacac caccatctcc agtcagatca
      421 tgttcgcccg cctcgccgag ttcttcggcc tgctcgccgt cgtgctcgtc gccacgggcc
      481 tctatggaac gctgtcctat cgcgtcaatc gccgcaccgc ggagatcggc gtgcgcatgg
      541 ccatgggcgc gggtcgcggc caggtggtgt ggatgattct gcgcgacagc ctgcgcctca
      601 cggtgatcgg cgtagtggcc ggcgtgccgc ttgcgatgct cattggccgt gccttatctt
      661 cctctctcta cggcgtcaag ccgctcgacg cgctcaccta cgcatttgcc attgcaggag
      721 tcgccgtcgt ggccctcttc gccagtgccg cacccgccag ccgcgctgcc agcgtcgatc
      781 ccctcaaggc gcttcgcacg gagtaatgct caactcagcg gctcctgccg ataaagttcg
      841 gccgtgtgcc gtactccgac agcaatcgcc atggcgagca tcgccgctgc cgcaatggcg
      901 ccgcccacat caaacagcag ccagcgatgc cccagcaccg ttgcataggg gctgcgcagc
      961 agtgccacat tgccagcgat caatagaatg cgaatctcgg ttggcccgaa gattccctgc
     1021 gacatctgaa aacacgacag agtgtaggtt gcaagatagc tctctgccga aagcagcaga
     1081 aacgcaatca gcaccgccag cgccacctgc caatgcacaa accccgaaag accaagcccg
     1141 cacatcatcg ccgttgcgcc cagcatatcc accatgtggt caacataaaa tccatagcgt
     1201 ggccgctgct ggttgcgcac tcgcgccagc gttccatcca tgctgtcgcc aaaccaattc
     1261 ataacaatgc atacaatcac gcccaccaac ccggcatcgt tgtagcgcga aagagcatag
     1321 aagaccccgg ccccgatctg cgccagcagt cccagcagcg tcagtccatc cgaggttacc
     1381 cagcccggcg cccgctccgc catccatgtc agtgcgcgcc tctccattcc ggcagtcagg
     1441 gagcggttga cgcgtcgggc agattcgaat gcccgcgtat tggcgatcgc gatgttcgac
     1501 ttcattctcg tctcctttcc gtgtctccgt tatctgcgca gtgcatcgca gaccgcatgc
     1561 agcgtaaact ccagcgactc acgcggcacg ctctccacaa acggccgaat tacccacccc
     1621 agtcccgccg gaatggatcg cgtcaacgaa gccgattcga tctgcacata gatgccgccg
     1681 tcgcgttctt cgcaactcca gtacgtattc agccgccaca ggaatccgtg ctcctcgctg
     1741 ccgctcagtg cgtgctcctt cgctgtgccc ggcgagccga tctccgagat gcgcgtactg
     1801 cgcgagatgc tgaaggcatg ttgtgcgtca agccgcctga aggtcacgtc atacgtcgta
     1861 tccatgacaa cggtgatcac atggtgctgc cgaacgcgca tcaataccat gaagtggttg
     1921 ggatctccga cttgctccgg cagaatcttc gcctccatca cctggggcga aaagcgctgc
     1981 gggtaagcgg cgtaattctt catcaggcgc tcaaagtccg ccacgcttgc tccgcgaatg
     2041 aacgcggttc cgcgccagtc gtgcagcatc gcgccacgca aatccgcctc cccggtcggc
     2101 gtgagtttct caatgatcaa ctcgcccttc cggaggcgag aatcttcagg accattgaat
     2161 ccgacggtgg cgacaaaccc gtttcccgat ctgtgctgct ccccgagccc cgcctccagc
     2221 ttcgctgcgt aagcgttgaa tgccgcggtc gccgtggccg gcgcctcacc cgccagccat
     2281 ggcgtcagaa tcattgccca gatcaggaga ccgaccgcct gaaaacccgt tcgcctgcct
     2341 tcgatccgac gtttcatgtc tcccagattg cctgtctcga cccc
//
LOCUS       DASZAK010001346         5031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_69305, whole genome shotgun sequence.
ACCESSION   DASZAK010001346 DASZAK010000000
VERSION     DASZAK010001346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69305"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VGT44_23560"
     CDS             <1..265
                     /locus_tag="VGT44_23560"
                     /inference="COORDINATES: protein motif:HMM:NF019228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEV2583852.1"
                     /translation="WNKIEYQFLAFVIQNWCGRSLISHEVIIKLILQTTTPARPTMQC
                     DLDANIDQSGYSDSDAKMAQVLLMRDVFHGEWNYTIIPHRVSK"
     gene            540..>5031
                     /locus_tag="VGT44_23565"
     CDS             540..>5031
                     /locus_tag="VGT44_23565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2583853.1"
                     /translation="MPDSYKSTEDPQTMTQHASNGTFQKEEHNGEVNVDVAEDSPDNT
                     ESWHVFSHTISVKMGFPGSESWSQEDGHRAEVSSDTFELEEEIQPQQNGHRAEVSSGT
                     LELEEETDKPSVDANHQQETMHRTIILAEDKNGHQHINSPAQQPVLLRPKHERRSYPT
                     DLTDAQWLKIAPLLPPSTADGHQHHVELREVVNGILYAVSAGHSWGSLPRDLPPRGTV
                     YSYFKRWQTGGALQQMFAALSLEESAAAAHARQDSFDAVSGHPAAEDAAPAGLLAVEV
                     ILEETTALPDVSEHQDVSHPLLSTLHDLSVVVPTRNEQDNIRPLLESLREALRDLRVE
                     VIFVDDSDDETPAVITEASRTLSSSQFQIHLEHRVAGEARAGGLATAVVRGMNRAQAE
                     FVAVIDADLQHPPELLRTFYDQAVAQDADLVLASRYIKGGSYRGLASVGRRAISKGLK
                     WTAKVLFPGQLLRISDPLGGFFLLRRSLLANVSLRPIGYKILLEILIRCQWRQVLEVP
                     YHFQARAAGQSKANMQQGIQVLQHMQRLWREVPAAGRVWKISFLILVNVSITLLLLLV
                     NKSFPLLWTNLNIVVFALTACLDFVLFNRFIFPLPIATSSAAPSSSSLISIDVIEEVL
                     KEDVLPSDQNGATNGYHPSSKDLIRADLEKDVLPSTQNGATNGHHPPSIDVIEEDLGQ
                     DELPSKELVVSSAVDLIRNSQPPRKKFSYDKFQSLAVVVVIILAVCWISYTQPGALLV
                     LAVLLIGFAIVITKNVNRDQAITMVLAVAVGVASIDYISWRIVATNWHGWWIAVPLLC
                     AEFFGALHVLGYQFTIWPRPPVEIEQGKDPTQYPIFVFIPTVNEGAAILRQTLEGIIA
                     ARAKYLAKYPHGRVTIVVCNDGRVAKTSNWEEIDRLAKELGVCCVTRTEGGGAKAGNI
                     ENARRELQATGDALLVIFDADQVAKPDFLLKTIPPFRDLKMGWVQTGQYYANLDNPVS
                     RWADDQQSMFYNLLCPGKEALNAAFICGTNVVIRASALDEIGGLPQDSVTEDFKASIA
                     LHPSWRSIYLTDILATGLGPLDIPSYLKQQGRWALGTLGVFRSHWREILLPRKHGLQI
                     GQRVQYFLACTHYLCGLRDLIYMVSPILFIFTGIPAVRSAYLSEYLWHFIPYGVLGAV
                     ALWYSARGVTGLRGIIIGFGSFPVLIGSLLSVVLQRKVGFAVTSKQHSGKRSLSYLRI
                     YFFFLLLCVDCLFWATRVKGQQETALFISVLWVVYSMLMLGSFLWLNLKDRRFQTALQ
                     QSGATDEIIAKHPYPSKLLLRPRGLHSVLNLGFAALIAVPILASSSQGILSFLVQSEA
                     TPFVISQDKIAAPYFGVSLPIQLLKSRPQVLEHDLDTQFSIIGRTQDIHDLFDASWAD
                     QLAAQHARPWITLQFGVFGPNQKPPLDANLPAIINGLHDNEIRRWAGDIREYGKPVYL
                     TVFQHADKNWSLSSGVANGGIPQDVPKAWMHVQSIFRAVGANNVAWVWAPADPINDQA
                     YAP"
BASE COUNT         1137 a         1308 c         1362 g         1224 t
ORIGIN      
        1 gtggaacaag atcgaatatc agttcctcgc cttcgttatt cagaattggt gcggtcggtc
       61 tttgatcagc catgaggtta ttatcaagct gattctgcag actacaacac cggcgagacc
      121 cactatgcag tgtgatctcg acgcaaacat cgaccaatca ggatatagcg attcggatgc
      181 caagatggcg caggttctgc tgatgcgaga tgtatttcac ggagagtgga actataccat
      241 tattccgcat cgcgtctcca aatgagacac taattttgtg cattttatta gagtatcatg
      301 ggtaacagcc cccctaaccc aatactctaa atttgaaaaa gctcatcccg aactcaaatt
      361 ccagcggatg cctaaacaga gagaaccttt gtttgttaca acatcatttc atgctgggcg
      421 caaagcactg gggataactg ctgacattgg gcagtatcgc gatctcgtta gatcaaacgc
      481 gtaggagatg tgagcatcca ggcttaccgg tcccttgtaa atgactgaaa gagatagaca
      541 tgccagattc ctataagtcg actgaagacc cacagaccat gacacagcat gccagcaacg
      601 gcacgttcca gaaagaggaa cacaatggag aggtcaacgt ggatgttgct gaagactccc
      661 ccgataatac tgagtcatgg catgttttct ctcacacaat aagcgtgaaa atggggttcc
      721 cgggcagtga atcttggtct caggaagatg ggcatcgtgc agaggtgagc tctgacacct
      781 ttgagcttga ggaagaaatt caacctcagc aaaatgggca ccgtgcagag gtgagctctg
      841 gtacccttga acttgaggaa gaaacagata aaccctctgt ggacgcaaac caccagcagg
      901 aaacaatgca ccgcacaatt atccttgctg aagacaagaa cggacaccag cacatcaata
      961 gccccgctca acagccagta ttgctcaggc caaagcacga gcgcagaagc tatcccaccg
     1021 atttgaccga tgcccaatgg ctcaagatcg cccccctgct gcccccttcc acggccgatg
     1081 ggcaccagca ccatgtcgaa ctgcgcgagg tcgtcaatgg cattctctat gccgtttcgg
     1141 ctgggcacag ctggggctcg ctgccccgtg atttgccgcc cagaggcacg gtctactcct
     1201 attttaagcg ctggcagacc ggcggtgcct tgcaacagat gttcgcggcc ctctcccttg
     1261 aggaaagcgc ggcggcagca cacgctcgtc aggactcttt cgatgcggtg tccgggcatc
     1321 ctgctgctga agacgcggcc cctgctggtc tactcgctgt cgaggtgatc cttgaggaaa
     1381 ccaccgcgct acccgatgtc agtgaacacc aggacgtttc tcaccctttg ctctcgacct
     1441 tgcacgatct cagcgtcgtc gtgcctacca gaaacgagca ggacaatatc cggccgctct
     1501 tagagtcact gcgtgaggcc ttgcgtgact tgcgggtgga agtcatcttc gtggatgata
     1561 gcgatgatga gactccagca gtcatcaccg aggcctcacg cacgctgagc tcgtcgcagt
     1621 ttcagattca cctggagcac cgggtagctg gagaggcgcg cgccggggga ctggcaaccg
     1681 ccgtggtgcg tggcatgaac agagcgcagg ctgaatttgt tgcggtcatc gatgccgatc
     1741 tccagcatcc cccggagctt ttgcgtacgt tttatgacca ggcggttgcg caggatgcgg
     1801 acctggtatt ggctagccgc tatataaaag gggggagcta tcgggggctg gcgagcgtgg
     1861 ggcggcgcgc tatttccaag ggcctgaaat ggacagccaa ggtactcttt cctgggcaat
     1921 tgttgcgtat atcggatcct ctgggcggtt tttttctcct gcgccgatcg ctactggcca
     1981 atgtatcact gcgcccgatc ggctacaaga ttctgctgga aattctgatt cgctgtcaat
     2041 ggcggcaagt gctcgaagtc ccgtaccatt ttcaggcccg ggctgctggg cagagcaaag
     2101 caaatatgca gcagggcatt caggtcttgc aacacatgca gcgcctgtgg cgcgaagtgc
     2161 cagcggccgg acgtgtctgg aagattagct ttctgattct tgtgaatgtg tcgattacgt
     2221 tgctgttatt gcttgtaaac aagtctttcc cgttgctatg gaccaatctc aacatagttg
     2281 tctttgcatt gacagcatgt ttggactttg tcctcttcaa ccgcttcatc tttcctctac
     2341 ccatcgcgac aagcagtgct gcgccatcat catcctcttt gatatccatt gatgtgatag
     2401 aagaggttct aaaagaggat gtgcttccat cggatcagaa cggcgcgact aatgggtatc
     2461 atccatcctc caaagatctg attagagcgg atttagaaaa ggatgtgctt ccatcgaccc
     2521 agaatggagc caccaacggg catcacccac cctctattga tgtgatagaa gaggatttag
     2581 gacaagatga gcttccatcc aaggagctcg tcgtatcctc cgcggtcgat ctgatacgta
     2641 attctcaacc gccaaggaag aaattttcct acgataagtt tcagtccctg gcggtagtgg
     2701 tagtcataat cctggcggtt tgctggatca gctacacgca gccaggcgca ttactggtcc
     2761 tggctgtcct gctaattggg tttgcgatag taatcacgaa aaatgtcaac cgcgatcagg
     2821 caattactat ggtgctggct gtcgcggttg gagtcgcctc aatcgactat ataagctggc
     2881 ggattgtcgc gacgaactgg catggctggt ggattgctgt gccgctgctg tgcgcggagt
     2941 tttttggggc acttcatgtg cttggttatc aatttaccat ttggccccgg ccacctgtgg
     3001 agattgagca gggtaaagat ccgacacagt acccgatttt cgtctttatc cccacagtca
     3061 atgaaggtgc cgcgattctc agacagacgt tggaagggat cattgctgcc cgcgctaagt
     3121 acctggcgaa gtatcctcac gggcgagtga ccatcgttgt gtgtaacgat ggtcgtgtgg
     3181 caaagacgtc aaactgggaa gaaattgaca ggctggcgaa agaactgggt gtctgttgtg
     3241 tgacccgcac cgagggggga ggagcgaagg cgggcaatat tgaaaatgcc cgccgggaac
     3301 ttcaagctac aggtgacgcg ctgctggtca tcttcgatgc cgaccaggtc gcgaaaccag
     3361 atttcttgct caaaaccatt ccaccttttc gtgatctcaa gatgggctgg gtacagaccg
     3421 ggcaatacta cgcgaacctg gacaacccgg tatcccgatg ggcagatgat cagcaatcca
     3481 tgttttataa tctgctgtgt ccgggaaagg aggctctcaa cgccgcgttt atctgtggaa
     3541 cgaatgtagt tattcgcgcg tctgcgctgg acgagatcgg tggcttgccg caagattcgg
     3601 tgaccgagga ctttaaagcc tctatcgccc tgcatccttc ctggcggagc atctatttga
     3661 cagatattct cgctacgggc ctggggccgc tggatattcc atcatatctc aaacaacagg
     3721 ggcgctgggc gctgggcaca ctgggcgtgt ttcgctccca ttggcgtgaa atcttgctcc
     3781 cgcggaaaca tggcttgcag attggacaac gagtccagta cttcctggcc tgtacgcatt
     3841 acctgtgtgg tctgcgtgac ctgatctaca tggttagccc tattctcttc atctttacgg
     3901 gtatccctgc tgtgcgcagc gcgtatttga gcgaatatct gtggcatttt atcccatacg
     3961 gtgtgctcgg cgccgttgcg ctgtggtatt ctgcgcgtgg ggttaccgga ttgcgcggaa
     4021 taatcatcgg ctttggcagc tttcccgtgt tgatcggaag tctgctctcc gtcgtcttac
     4081 aacgcaaggt cggttttgct gtgacctcga aacagcatag tgggaagcgt tcgctcagct
     4141 acttaaggat atacttcttt ttcttgctgc tctgcgtcga ttgcctgttc tgggcaactc
     4201 gggtcaaagg tcagcaagag actgctctct tcattagcgt gctgtgggta gtttactcga
     4261 tgctgatgct gggcagcttc ctctggctga acttaaaaga tagacgcttt caaacggcct
     4321 tgcagcaatc aggggcaact gatgagatca ttgccaagca cccatatcct tcgaagttat
     4381 tgctgcgccc acgcggacta catagtgtat tgaacctggg atttgccgca ttgatagccg
     4441 tccctatcct cgcgagtagc tcccagggca ttctgtcatt tctcgtgcag agcgaggcca
     4501 cgccgtttgt gatcagccag gataagatag ctgcgccata ctttggcgtt tcgctcccga
     4561 ttcagttact aaagagccga ccacaagtgt tggagcacga tctggatacg cagttttcaa
     4621 tcatcgggcg cacgcaagat atccatgatc tgtttgatgc atcctgggca gaccagctgg
     4681 cggcacagca tgcacgtccc tggatcacgc tccaattcgg agtgttcggc cctaatcaga
     4741 agccgccgct cgatgccaat ttgccggcga ttattaacgg acttcatgat aatgagatca
     4801 gaaggtgggc cggggatatt cgcgagtatg gcaagccagt ctacctgacg gtctttcagc
     4861 atgccgataa gaactggtcg ttatcatcgg gtgtcgccaa tggtggtatt ccacaggatg
     4921 taccgaaggc atggatgcac gtacagtcca tctttcgtgc cgttggagca aacaacgtag
     4981 cctgggtctg ggccccggct gatcctatca atgaccaggc gtatgcacca c
//
LOCUS       DASZAK010001347         1520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_495943, whole genome shotgun sequence.
ACCESSION   DASZAK010001347 DASZAK010000000
VERSION     DASZAK010001347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495943"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..1391)
                     /locus_tag="VGT44_23570"
     CDS             complement(303..1391)
                     /locus_tag="VGT44_23570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV2583854.1"
                     /translation="MEDQAKMNVGIIGCGTISPIYLQAPAKFALLRIVACADLIPEVA
                     QAKARQYGVPKVAMVEELLSDPEIDLVLNLTVPAAHTEIGLAALAAGKAVYSEKPLGI
                     NREQGKALLEAGRARQRMIGCAPDTFLGGGLQTCIKLINDGAIGVPIGATAFLLCHGH
                     ESWHPAPDFYYQPGGGPMFDMGPYYLTALIAMLGPVRRVTGSTRITFPERVVTSQPNP
                     GKVIKVQIPTHIAGVLDFANGAIGTIITSFDVWSHQLPHIEVYGTEGTLIAPDPNGFG
                     GPVYVRGAQERAWREVLHTHGYTENSRGIGAADMAHAMRTGQTPRASGELAYHVLDIM
                     EAIHDASREERHITLSSTCQRPAPLLPQ"
BASE COUNT          285 a          438 c          451 g          346 t
ORIGIN      
        1 tgggcacaat caggtcgagg ctctgcattt ttgcttcatc caggtacaca tccagggtgg
       61 tggcgatttc caggtggtag ccctggacgc gtaaaaaagc ggcgaagatg gccgcgcctt
      121 tttctggctc gtgcccttcc cagcctcccc agataatcag tgctgatttc atgagatgga
      181 ttctttcttt gtagttattc agctccccct cttgccacct gcggcgataa aagaagcaaa
      241 agagagatgt gggaacatcc caaaccccga caagtcaatg tccctgcacg ctacttgatc
      301 atttactgag gcagcagtgg cgctgggcgc tggcaagtgc tgctcagggt gatgtggcgt
      361 tcttcgcgtg aggcgtcgtg gatcgcttcc atgatgtcca gcacgtgata ggccagttcg
      421 ccgctggcgc gaggcgtttg cccggtgcgc atggcgtgcg ccatgtctgc tgcgccgata
      481 ccgcgactgt tctcggtata accgtgggtg tgcagaactt cgcgccatgc ccgttcctgg
      541 gcgccacgca catagacggg tccgccgaag ccgttgggat cgggggcgat cagcgttcct
      601 tcggtgccgt agacttcgat gtgtgggagc tggtgggacc agacatcgaa gctggtaatg
      661 atggtgccaa tcgcgccgtt cgcgaagtcc aggacgccgg caatgtgtgt ggggatctgc
      721 accttgataa cttttcccgg attcggctgg ctggtcacca cgcgttcggg gaaagtgatg
      781 cgcgttgacc cggtgacgcg ccggaccggt ccgagcatgg cgatcaaggc agtcagataa
      841 tacggtccca tgtcgaacat tggtcccccg ccgggctgat aatagaagtc cggggccggg
      901 tgccagcttt cgtgaccgtg acacagcaga aaagccgtcg caccaatcgg cacaccgatc
      961 gcgccatcat tgatgagttt gatgcaggtc tgcaagcccc cgcccaggaa ggtatccggg
     1021 gcgcacccga tcatacgttg cctggcccgg cctgcctcca gcagagcttt gccttgctct
     1081 cgattgatgc cgagcggctt ctcgctatag acggctttgc ccgccgcgag cgccgccagt
     1141 ccgatctccg tgtgggctgc aggcacggtg agattcagca ccagatcaat ctccggatca
     1201 gagagcaatt cctcgaccat cgcgaccttt ggcacaccat actgcctggc tttggcctga
     1261 gctacttccg ggatcaggtc tgcgcaggcg actatccgca gaagcgcgaa ttttgcaggc
     1321 gcttgcaggt agatgggact gatggtgccg catccaatga tgcccacatt catttttgcc
     1381 tggtcttcca tgagcgcgct cctcttttct cctctaccgg agtgttcctg ggaaaatgga
     1441 tgctatccag gagcttcctc tagcattgaa ccgcattttc agaggaacgt caactgccga
     1501 attgcctcag tgtgatcgtt
//
LOCUS       DASZAK010001348         9701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_34445, whole genome shotgun sequence.
ACCESSION   DASZAK010001348 DASZAK010000000
VERSION     DASZAK010001348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 9701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9701
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34445"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..858)
                     /locus_tag="VGT44_23575"
     CDS             complement(100..858)
                     /locus_tag="VGT44_23575"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TdeIII family type II restriction endonuclease"
                     /protein_id="HEV2583855.1"
                     /translation="MAATKDLINQVMQNQLGAFANNFKRDYSTGGTKLKNLVNKANNP
                     FVLALGQEIVIYSTLMRSLDSSLGNCLERIARAIAESSYYVYDVVEGDVPIGIDQKIS
                     DLMNMYTSKRRKPQLSDLDEITITATGQTEHKIHKSDYHLVKRSDESQHFLLELKIGG
                     DLDNKKARSEKQALLEQYAILKYSPRIRNDSSIRLYFATAYNFSGEDEIWSQERVRQF
                     FSEDELLIGRDFWNFISDSSEGTAPLTGEIREKD"
     gene            1083..1796
                     /locus_tag="VGT44_23580"
     CDS             1083..1796
                     /locus_tag="VGT44_23580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV2583856.1"
                     /translation="MTINNHVAIITGASRGIGRATAQRFAREGARVVLFSRTAAALEQ
                     VAAEIAREGHAALPIAGDVSREGDVQALFQWAIERYGRVDILVNCAAIVAVQSFAGMD
                     IATWDAVIDTNLRGTFLCCREAFRVMSEQRSGVIINLSSLSGVKGVEKFPGLSAYNVS
                     KAGVASLTEILAVEGKPYNIRVCAVSPGAVDTEMLRQAAPHLKAGMTPGDMADILLFL
                     ADDSGRALSGINLEIFSNS"
     gene            1817..2938
                     /locus_tag="VGT44_23585"
     CDS             1817..2938
                     /locus_tag="VGT44_23585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV2583857.1"
                     /translation="MMRIALVAPLVSPIVQPFLGGSQALLADLAQGLTRRGHDVTLFA
                     RDTSFVPNVTIETIAVPGGVRLEPASFAPDIDANATRAADAGFFTQANLFLDLFLQLQ
                     QRSAEFDVVHMHAFDWPAYACSPLIRDIPVLHTLHLPAVSSEINAALKALDRRGHPVT
                     LVAVSHACALTYSPYTAIDAMIYNGLELDAIPFSPRPSKEAPLLFAGRIAPEKGVEAA
                     IDIAAMAGYRLLIAGGIYDRLYFEERIAPRLLEAGERVTYLGQLERLALWKIMGQSLG
                     LLFPIEWDEPFGLTAVEAMATGTPVIAYRRGAAEEVIRHGETGYLVEEGERTQAAALV
                     EDLYDIPRVGCRAHVEAHFGMDAMIDAYERVYAEATQFI"
     gene            complement(2951..4345)
                     /locus_tag="VGT44_23590"
     CDS             complement(2951..4345)
                     /locus_tag="VGT44_23590"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEV2583858.1"
                     /translation="MFRKHMIFSLGGIALCLMMLAVGLMMSGPGHSHAASSLGSARTP
                     SISHQLAQPFDAVQGNIYYGTGSTSVYALDAQTGAQLWVYPLQCGNGMNFFTVLWVVN
                     GIAYVNTCNPSCLSHLAAIRGSDGTQLWCSTFTFFAPGANGTRFLGVSQGIVYLYTVI
                     PGGDGGEHDTLHALNATDGSLLWSYDQNPQIPDQFLDFFKVVQGVAYISMDQYNPAGG
                     GPYLTCALNTSSGSVNWCAANALTSILAQGVLYATNSTSLIALRASDGSQLWSYAGSF
                     DQIVAHPKVVFVGGPGSLCALNSSNGSQRWCSSVNAYHFFVQGGIIYAGGQAFSASTG
                     ALLWTNQAANIIGLGQSVVYALTGGYYVEEINAMSGSLLWSYSIAQSGTASFSPGDGV
                     IYYNTPHGLRALSASTGTLLWSHFTTSNAILSFQSDSSAIAYVVSNNTTTNSFTLHAY
                     NASNGTLLWQYAMP"
     gene            4697..5728
                     /locus_tag="VGT44_23595"
     CDS             4697..5728
                     /locus_tag="VGT44_23595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV2583859.1"
                     /translation="MYSRSAYPTPVAVPTAAKGTRTGRLFRRVIVALLIVSGMVTLAY
                     STLSIYIATQLVYVPPTPIYATPASLGLQFKDVTFPSRVDQVQLRGWFIPGVLSGGRL
                     TAQRTIVVVHGSRTNRADQAAGLLNLSGQFARHGFAVLAFDMRGMGESPPAPLSLGYF
                     EQRDVLGAVDFLRSGPIPYPDLGRPRLIGGWGVSMGAATLLLAASQEPAIRAVVSDSA
                     YADILPILEREVPKGGHLPPLFTPGALLAARALYGMDFYAVRPVDVVARLAPRPVFFI
                     HGTGDTYIPQVNMALLAAAARTAPDASVQTWLVPGAQHAQAFNTHPQEYVARVMAFFT
                     NALGPDTNP"
     gene            5754..6149
                     /locus_tag="VGT44_23600"
     CDS             5754..6149
                     /locus_tag="VGT44_23600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583860.1"
                     /translation="MSAHAFQRSNVPAQNHPQGGLARGVLLGLIPLALLLALVVAALL
                     LTALARQLTAASGFFTQQQVSLIVLIGGLALAAVVYSVALVLTLRHVASWQPGGAQAR
                     TALLALGITALIVVLPVLLALLLPQAPAP"
     gene            6160..6711
                     /locus_tag="VGT44_23605"
     CDS             6160..6711
                     /locus_tag="VGT44_23605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583861.1"
                     /translation="MLRPHSRLTSLAVTGCLLVLLVACGPFGPADTGPTPTASTVGAT
                     ATPTRGRAASTPTTVPMPATQTACPSPARAAVFAPLVLGSHQTIVYLFNQAGASGSAE
                     IKRYDASTGKKVIILNETSQFPWSNVSRDGSRYVLKITSNNGPTQTTQSLLIGSLGGA
                     SPSTFATVSSTVGSVAIVGWTTM"
     gene            6768..8195
                     /locus_tag="VGT44_23610"
     CDS             6768..8195
                     /locus_tag="VGT44_23610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583862.1"
                     /translation="MLSRRRAARNTRAALSSWSVRIATCAILSVALITLAFPTAPAAR
                     AVQLGGSQPIIHWAANMITPVQNNGNPWGPVGELTTVNGENFTPNVQLRLVLAPGDSN
                     TDATLCQQPGVTVISSTVFTNSTGRFGTAEGFEGFYWPAAANQVNQGYSICSVLASDH
                     SLASSHDDGPFTVQSASPPAIEYSRTNVAAGGTVIFTGQNWVPPQQVLIDLTGCSACS
                     PRNGLITIGSVTSTGLNSGTFQIAIVIPRTIQPANYTVDALAHAPYLEAYNDQILSIF
                     PGSTTPTLTPTPLSSPLPGPSPTSSAIVPSATPAASATTRGASGTVATTSNGSGGTTG
                     TAGDTSESGILIALICVALGFFLVAAVICVMLLLRRKRAGHAAQDPPYYSAPQAGQFG
                     QPGQVGQSNLANNPSPFFAQPGASLVPAEYFTPLPSSPLQQSAMASPYGQSLHLPTCP
                     NCGRSLVPNQPACSVCGMPLSAMRW"
     gene            complement(8210..>9701)
                     /locus_tag="VGT44_23615"
     CDS             complement(8210..>9701)
                     /locus_tag="VGT44_23615"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2583863.1"
                     /translation="GIVHRDVKPQNILIGRGGSIKLTDFGIASVYKDINSERLTTTGM
                     TLGTVQYYAPEQAQGEIVSSAADVYALGIVMYEMLVGHTPFDGDTPVAVAMQHIQDAP
                     IPPSQFNPNIPPALEEIILRCLQKTPEMRYRDGSQLARALEALGDAEFSETIPYTPGS
                     LPVQGGQIPARPSGSGRAPAQKSQVSTSSPDHALPVGVSSERNGASPAPIPVSQQHLY
                     LDQQAYAPPVPPIAPPHSPAESGSTRPHALNVARTQERHASRFATVITVLILLATLLL
                     MFFSIYLAVLLGFINWPFSNNSQVTPTQTIEHVVPDLVGKYYPTAQQIAKSDGFVLQV
                     SGSTSIGYVKSQYPLPPYKLAAGGTITVTLTTSTKVPSIPPGANLEQMQLILQNAGLK
                     YNVLSDGRKTSVPLNTISRLDPTPGSPIEVGATVNIYVWNLGATPASSPTPSPTHNPA
                     PSPVPSPTPSPTPSPTPSPTPSPTPSPTPSPTPSPSPTIGPTPTPM"
BASE COUNT         2002 a         2751 c         2769 g         2179 t
ORIGIN      
        1 gcggaacacg ccgagttcgg gcaaaattgc ttcgacgcgt ccatcggctc tggtgtactg
       61 ttttgcataa gcacatcttg ccgaggagcg tcgttttttt caatccttct cccttatttc
      121 accggttagt ggggctgtac cttcactgct atcactgata aaattccaga aatcacgtcc
      181 tattaacaat tcatcttcac tgaaaaattg ccttacacgt tcctgactcc atatttcatc
      241 ttcacctgaa aagttgtaag cagttgcgaa gtaaagcctg atactactat catttctgat
      301 acgtggggaa tatttaagta tggcgtactg ctcaagcaat gcctgttttt cactacgagc
      361 ctttttatta tctagatcgc cgccaatctt taattcaagt aggaaatgtt ggctttcatc
      421 agacctcttc actaaatgat aatcactctt atgaatctta tgttctgttt gtcccgttgc
      481 tgttatcgtt atctcatcaa gatcgctcaa ttgaggttta cgccttttag aagtatacat
      541 attcattaga tcggatattt tttgatctat gcctatcgga acatcgccct ctaccacatc
      601 atagacataa taacttgatt cagcaatagc acgtgcaata cgctcaagac aattccccaa
      661 tgaactatca agcgacctca ttaatgttga ataaataact atctcctggc caagagctaa
      721 aacgaatgga ttgtttgcct tatttactag atttttcagt ttagtcccac cagttgaata
      781 atctcgtttg aagttattag caaaagcgcc taactggttc tgcataacct gattaattaa
      841 atcttttgtt gctgccattt attcaccgct tgctatgctt gatttcgcta cattgaattg
      901 gcttgaaggc aatgtatttt ctaggataac cggactgtag catacttatc aactcaagtc
      961 gagataagcg cctgaaggta atattctagt gggtcctcat taaggattca aggaccattt
     1021 tttaccgagt atctcaatat taactcaacc aggaacggaa aacgacgtgc tataataccc
     1081 ctatgaccat caacaaccac gtcgccatca tcaccggcgc gtcgcgtggc ataggccggg
     1141 cgaccgcgca gcgcttcgcg cgcgaagggg cgcgcgtcgt cctgttcagc cgcacagcag
     1201 ccgcgctgga gcaagtcgcg gcggagatcg cgcgtgaagg acatgctgcg ctccctatcg
     1261 cgggcgatgt ctcgcgtgaa ggtgatgtac aggcgctatt ccagtgggcc atcgagcgct
     1321 acgggcgcgt tgatatcctc gtgaactgcg cggctattgt ggccgtccag tcttttgcgg
     1381 gcatggatat cgccacctgg gatgccgtga tcgataccaa cctgcgcggg acattccttt
     1441 gctgccgtga agcgttccgc gttatgagcg agcagcgtag cggcgtcatc atcaaccttt
     1501 cctcgctttc cggcgtaaaa ggagtagaga agtttccggg attgagcgcg tataacgtct
     1561 cgaaagcggg cgtagcgagc ctgaccgaga ttctcgccgt cgagggcaag ccatacaaca
     1621 ttcgcgtatg cgccgtcagt cccggagcgg ttgataccga gatgctgcgt caggccgcgc
     1681 cccatctgaa ggcgggcatg acgcctggcg atatggccga catcctgctg tttctggcgg
     1741 acgattctgg gcgagcattg agcggaatca atttagaaat atttagtaat tcataatgtt
     1801 gagataatcg aggtgtatga tgcgtattgc gctggttgct ccactggttt ccccaattgt
     1861 tcagccgttt ctgggcggat cgcaggcgct gctggccgac ctggcgcagg gattgacgcg
     1921 acgcgggcat gatgttacgc tgtttgcgcg cgatacgtcc tttgtgccga atgtgacgat
     1981 cgagacgatt gcggttccgg gcggtgtgcg gctggaaccc gccagctttg cgccggatat
     2041 agatgcaaac gccacgcgcg ccgcggacgc gggctttttt acccaggcca atcttttcct
     2101 cgacctcttc ttgcaattgc agcagcgcag cgcggagttc gatgttgtgc atatgcatgc
     2161 cttcgactgg cctgcctacg cttgcagccc gctgattcgc gatattcccg tgttgcacac
     2221 gctacatctt ccggcagtct cgtcggagat caacgctgcg ttgaaggcgc ttgaccggcg
     2281 tgggcatcct gtcacgctgg tggccgtctc ccacgcctgc gccctgacgt acagccctta
     2341 tactgccatc gatgccatga tttacaacgg gctggaactg gatgccattc ccttctcgcc
     2401 caggccatcg aaagaggctc cgctgctctt cgccgggcgt attgctcctg aaaaaggcgt
     2461 ggaggcggcc atcgatatcg cggcgatggc gggctatcgc ttgctgatcg ccggcggcat
     2521 ttatgatcga ctgtacttcg aggaacgcat cgcgccgagg ctgctagagg cgggcgagcg
     2581 tgtgacctac ctggggcagt tagagcggct ggcgctgtgg aaaatcatgg ggcaatcgct
     2641 ggggctgctc ttcccgatcg agtgggacga gccgttcgga ttgaccgctg tggaagcgat
     2701 ggcgaccggc acaccggtaa ttgcctatcg ccggggcgcg gcagaagaag tcattcgtca
     2761 tggcgagacc ggctacctgg tcgaagaagg cgaacgcacc caggccgccg cgctggtcga
     2821 ggatctctac gacatccccc gagtgggctg ccgcgctcat gtcgaggcac atttcggcat
     2881 ggatgccatg attgatgcat acgagcgtgt ctacgcggag gcaacgcagt ttatctagtt
     2941 gcaggattat tcacggcatc gcgtactgcc agaggagcgt cccattactt gcattgtagg
     3001 cgtggagcgt gaatgaattt gttgttgtgt tgtttgacac aacataggca atcgcactgc
     3061 tatcactctg aaaggacaga atcgcattcg aggttgtgaa atgagaccag aggagcgtac
     3121 cagtactcgc gctgagggcg cgaagcccat gtggggtgtt gtagtagatg acgccgtcgc
     3181 ccggcgaaaa ggaggcagtc ccgctctggg caatgctgta gctccacagt aacgagccac
     3241 tcattgcatt gatttcctcc acataatagc cgcctgtcag cgcatagacg acgctttggc
     3301 ctagcccaat gatgtttgcg gcctgattcg tccataaaag agctccagta ctggccgaaa
     3361 acgcctggcc accagcatag atgatgcccc cttgcacgaa gaaatgatat gcattgacag
     3421 aggaacacca gcgttgagaa ccattactac tattgagcgc gcagagacta ccaggacccc
     3481 cgacaaagac gacttttgga tgtgcaacga tttgatcgaa tgatcccgca tagctccaga
     3541 gttggctgcc gtcgcttgct cgcagggcga tcagggaggt actgtttgtg gcatagagca
     3601 ctccctgagc aagtatgctt gtcaaagcat tggcggcgca ccagttcaca gagccgctac
     3661 ttgtattgag cgcacaggtc aggtacggac cccctcctgc cggattgtat tgatccatgc
     3721 taatgtaggc gactccctgg acaaccttaa aaaagtcaag aaactgatcg ggaatttgcg
     3781 ggttctggtc ataactccag agcagcgagc catccgtcgc gtttagcgcg tgaagagtat
     3841 catgttcacc tccatctccc cctggaatga cggtgtagag atacacaatc ccctggctga
     3901 ctcctaagaa acgagtgcca ttagcacctg gtgcgaaaaa agtgaacgtt gaacaccata
     3961 gttgggtccc atcgctgccc ctgatcgctg ccaggtggga gagacacgac ggattacagg
     4021 tgttcacata ggcaatgcca ttcactaccc ataaaacggt gaaaaagttc attccattgc
     4081 cacattgcaa cgggtaaacc cagagctgcg ccccggtttg tgcgtcgagg gcgtacacag
     4141 aagtactgcc cgtcccgtag tagatgttgc cttgcacggc atcaaaaggt tgcgctaatt
     4201 gatgagaaat actaggtgtc ctggcagaac cgagcgacga cgctgcatgg gagtgaccgg
     4261 ggccgctcat catcaagccg acagccagca tcatcagaca gagggcgatt cccccaagtg
     4321 agaagatcat atgcttgcga aacattcgac ttattctcca tttctggcga ggtggtggac
     4381 gccgatcatg ccttgagtaa gggccaattc gtgaaagtag ggagaaagat gagacgaagc
     4441 caaatctata gtagtgattg agagaggatg aagcggttga ggaagcatag tagttcggtt
     4501 aaatagtgtt aatgaaagta aagaattctc aatgccagga cagaacatgc gtttagtcga
     4561 ttgggagtta ctttctcccg ctgcttacgt ctccatagta gattaacact cgttcagctt
     4621 ccaacgactt ctttctcgca ccaaaggact gcgcatgtcg cagagaaaga cgtgttccac
     4681 cagggagggg cccatcatgt attcacggtc agcctacccg acaccggtcg cggtaccgac
     4741 tgcggccaaa ggaaccagga ccgggcgtct ctttcgacgg gtcattgtgg cgctgctcat
     4801 cgtatcgggc atggtgacgc tggcttacag cacgctctcg atctacattg ccactcaatt
     4861 agtctacgtg ccgccaacgc cgatttacgc gactccagcc tcgctcggct tgcagtttaa
     4921 ggatgtgacc tttccgagca gggtagacca ggtgcagtta cgcggctggt tcattcccgg
     4981 cgtcctctct ggcgggcgat taacggcgca gcgcaccatt gtcgtggtgc atggcagtcg
     5041 caccaaccgg gcggaccagg cagcggggct gctcaacctg agtggccaat ttgcccgtca
     5101 cgggttcgcc gtgctggctt tcgatatgcg cggcatgggc gaatcgcctc ccgcgccgct
     5161 ctccctcggc tactttgagc aacgcgacgt gttgggagcg gtcgatttcc tgcgctccgg
     5221 cccgatcccc taccctgacc tgggtcgccc gcgtctcatc ggcggctggg gcgtctcgat
     5281 gggtgcggct acgctgctgc tggccgcctc acaggagcca gccatccgcg cggtcgtctc
     5341 ggacagcgct tacgccgata ttcttcccat tttagagcgc gaggtgccta aaggcggaca
     5401 tcttcccccc ttgttcacgc ccggagcgct gctggcggct cgcgcgctgt acgggatgga
     5461 cttctacgcg gttcgcccgg tggatgtggt agcgcgcctc gcgccgcgtc ccgtcttctt
     5521 tattcacggc accggagaca cctacatccc ccaggtgaat atggctctgc tggcggcagc
     5581 cgcgcgcacc gctcccgatg ccagcgtcca gacctggttg gtgcccggcg cccaacatgc
     5641 ccaggctttc aatacgcatc cccaggaata tgtggcgcgc gtcatggcct tcttcaccaa
     5701 cgcgcttggg ccggatacca acccgtagtc atccaatcat cgaggaggaa gccatgtccg
     5761 cacacgcttt tcaacggtca aacgttccgg ctcaaaacca tccgcaaggc ggtctggcga
     5821 gaggcgtctt gttggggctg atcccactgg cgctgctgct ggcgctggtt gtcgccgccc
     5881 tgcttctcac ggccctcgcc cgccagctga ccgccgcgtc tggtttcttt acgcaacaac
     5941 aggtgtcgct gatcgtcctg attggcggtc tcgcgctggc tgccgtcgtc tatagcgtcg
     6001 ctctcgtgct gacgttgagg cacgtggcaa gctggcagcc tgggggggcg caggcgcgca
     6061 cggctctgct ggccctgggg ataaccgcct tgatagtcgt attgccggtg ctcctggcac
     6121 tcctcctgcc gcaagcaccg gcaccgtagg aggaaagtca tgcttcgtcc ccattcccgc
     6181 ttgacttccc tggcagtcac tggttgtctg ctggttcttc ttgttgcctg tggccccttt
     6241 gggcctgcgg atacgggacc aacgccgact gcgagtaccg tcggcgctac cgccacacca
     6301 acgagagggc gcgctgcctc gacaccgacc accgttccga tgccagccac acaaacggct
     6361 tgccccagcc cagcacgggc agccgtgttc gctccgctgg ttttagggtc ccatcagacg
     6421 atcgtctacc tcttcaatca ggcaggggcc tcaggctctg ccgaaatcaa gcgctacgac
     6481 gcatcgactg gcaaaaaggt gattattctg aacgagacgt ctcaattccc ctggtcaaat
     6541 gtctcacgcg acggcagcag gtatgtgttg aaaatcacca gcaacaatgg gccaacccaa
     6601 acgacccaat ccctgctgat cgggtcgctg ggaggcgcca gtccttccac ctttgcgacc
     6661 gtttcgagta cagtagggag tgtggcgatc gtgggttgga ccaccatgta ggtctgccgc
     6721 actaacgtgg aaggttcgag cgaccagacg gggagggtag aacgacgatg cttagcagaa
     6781 gaagagcagc caggaataca cgagcagcgt tgtcaagctg gagcgtgagg attgccacat
     6841 gtgccatcct cagcgttgcg ctcatcactc tggcattccc gaccgcgccc gctgctcgcg
     6901 ctgtccagct cggcgggagc cagccgatta ttcactgggc tgcgaatatg attacccccg
     6961 tacagaataa cggcaatccc tggggaccgg tcggcgaact cacgacggtg aatggcgaga
     7021 atttcacgcc gaatgtgcag ctgcgcctgg ttctcgcgcc gggcgatagc aataccgatg
     7081 ccaccctatg ccaacagccg ggtgtgacgg tcatctcctc tacggtgttc acgaactcga
     7141 cgggccggtt tggtacggcg gagggtttcg aggggttcta ctggcccgcc gctgccaacc
     7201 aggtcaacca gggctatagc atctgtagtg tgctggcgtc cgatcacagc cttgccagca
     7261 gccacgacga cggcccgttc accgtccagt cggccagccc cccggcgatc gagtattcgc
     7321 gcacgaacgt cgcggcgggc ggcacggtca tcttcacagg gcagaactgg gtaccgccac
     7381 agcaggtttt gatcgacctg accgggtgca gcgcctgtag cccgaggaat ggcttaatca
     7441 ccatcggcag cgtaacttcg accggactca atagtggcac ctttcagatc gcgatcgtca
     7501 tcccacgcac aatacagccg gctaattata ccgtcgatgc gctcgcacac gcgccttacc
     7561 tggaggcata taacgaccaa atcctgtcga tttttcccgg ttccactacg ccgaccctca
     7621 cgccgacacc actgtcctcg ccattaccgg gtcccagccc tacctcgtcg gcgatcgttc
     7681 cctcggcgac gcctgctgca tcggccacca cgcggggtgc aagcggcacg gtcgccacca
     7741 cctccaatgg ctctggcgga acgaccggaa ccgcgggcga taccagcgag agcggcatcc
     7801 tgatcgcctt gatctgcgtc gcgcttggct tcttcctggt cgccgccgtc atctgcgtca
     7861 tgcttttgtt gcgccgtaaa cgcgccggac atgctgccca ggacccaccc tattactctg
     7921 ctccgcaggc cggtcagttt ggacaacccg gccaggttgg acagagcaac ctggctaaca
     7981 acccatcgcc gttttttgcc caacccggtg ccagcctcgt tccggcggag tacttcacgc
     8041 cgctgccatc atcgcctctt caacagagcg cgatggcctc tccgtatggg caatcactcc
     8101 atctgccgac ctgtcccaac tgcggcagat cactggttcc caaccagccc gcctgcagcg
     8161 tctgcggaat gccgctgagc gccatgcggt ggtaaggaac cataagagat tacattggcg
     8221 ttggcgtcgg cccaatcgtc gggctgggtg acggagttgg gctaggtgta ggtgatggcg
     8281 tcgggctggg cgtgggcgac ggagttggcg atggcgtcgg gctgggcgtg ggcgagggga
     8341 ccggcgatgg cgcgggattg tgcgttggcg agggcgttgg cgatgaagcg ggtgtcgcgc
     8401 ctaaattcca gacatagatg tttaccgtcg ctccaacctc tatgggggaa ccaggtgtag
     8461 gatcaagtct ggagatcgta tttagaggca cagaggtctt gcgaccatct gaaagcacgt
     8521 tatatttcaa gcctgcgttt tgcagaatca actgcatctg ctcaagatta gcccccggtg
     8581 gaatactcgg aaccttcgta ctggtcgtca atgtgacggt gatcgtgcca ccggcggcca
     8641 atttgtaagg tggtaatgga tactggctct tcacataacc tattgaagtc gagccggaaa
     8701 cttgtaagac aaatccatca ctttttgcga tctgttgtgc agtgggatag tattttccca
     8761 ccaggtcggg aaccacgtgt tctatggttt gtgtcggcgt cacctgggaa ttattgctaa
     8821 agggccaatt gataaagccc aacaggacag ccaggtagat gctgaaaaac atcagcagca
     8881 gggtggcgag taggatcagt acggtgatga cggtcgcgaa gcgagaagca tgtctttctt
     8941 gcgtgcgagc gacgttgagc gcatgcgggc gcgtcgagcc tgactccgct ggcgaatgag
     9001 gaggagcgat aggcggaact ggtggcgcgt acgcttgctg atcgagatag aggtgttgtt
     9061 gagacaccgg aatgggcgcg ggactggccc cgttgcgctc agacgacacg ccgaccggca
     9121 gcgcgtggtc gggcgacgaa gtggagacct ggctcttttg cgccggggcg cgcccactgc
     9181 ccgagggacg cgccggtatc tgacctcctt gtactggcag tgaccctggt gtgtagggga
     9241 tggtttccga gaattcggca tcgcccagcg cttccaacgc gcgcgccagt tgcgagccat
     9301 cgcgatagcg catctcgggc gtcttttgca ggcagcgcag gataatctct tccagtgcgg
     9361 gtgggatgtt gggattaaac tggctgggcg ggatgggggc atcctggata tgctgcatgg
     9421 ccacggccac gggcgtatcg ccatcgaagg gggtatgacc caccagcatt tcgtacatga
     9481 caatgcccag cgcatacaca tcggcggccg agctgacaat ctctccctgt gcctgctccg
     9541 gggcgtagta ctgcactgta cccagcgtca tgccggtggt ggtcagccgc tcggagttga
     9601 tgtctttata gacgctggca atgccgaaat cggtcaattt gatcgagccg ccgcgtccaa
     9661 tcaggatgtt ctggggtttg acatcgcgat gcacgatgcc c
//
LOCUS       DASZAK010001349         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_210513, whole genome shotgun sequence.
ACCESSION   DASZAK010001349 DASZAK010000000
VERSION     DASZAK010001349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210513"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VGT44_23620"
     CDS             <1..457
                     /locus_tag="VGT44_23620"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HEV2583864.1"
                     /translation="RASLWAQELVQQPREPAPLAREREDVARARILLAQAKPTEALAL
                     LEPLRGGAQRQGRWNHVIEMQVLRALAHSMGDQEQEALAVLAQAVQLAEQEGYVRIFV
                     DEGAPMARMLSRLREQEDRQVPTGYVDTLLAAFGEVGGVHDDATGAGPL"
     gene            573..782
                     /locus_tag="VGT44_23625"
     CDS             573..782
                     /locus_tag="VGT44_23625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583865.1"
                     /translation="MEQRLESDLVKRLEQLGIEVDVSPTGTTLTYEQIYTSGPTLEEA
                     LLTFLGEALSRYEALRTWKASMHDC"
     gene            complement(779..>2306)
                     /gene="ftsH"
                     /locus_tag="VGT44_23630"
     CDS             complement(779..>2306)
                     /gene="ftsH"
                     /locus_tag="VGT44_23630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV2583866.1"
                     /translation="LLIYLGYMALRSFRQSQQGGGIFGVGRSRAKLYNEERPSTTFAD
                     VAGADEAKADLQEEIEFLREPQRYANIGAHIPKGVLLVGPPGTGKTLLARAVAGEAGV
                     PFYSASGTGFVELFVGVGASRVRDLFEQAKKNAPCIIFIDEIDAIGRTRSGASAFASN
                     DEREQTLEQLLVEMDGFEPTQAVVVLAATNRPDVLDPALLRPGRFDRQITIDLPERRG
                     REAILRIHTRNVPLAPEVNLADLARVTPGFSGADLENLVNEAALAAARRDSRQVSRQD
                     FEHALDKLLLGGKREALMDERERRTVAYHEGGHALVAAVLPDVDPIYKVTIVPRGRSL
                     GVTQFRPDDDRRNLPRSYLLERLAVALGGRSAEELALGDITTGAENDLKEATQLARRM
                     VSEWGMGEHTGPVVYALSDGSPSLSQQPLEGHERVYSEVTAERLDAEVEQLMTQAHQQ
                     ARSVLTEHREALDRLAQALQQEEVLERDQVLAIVHGGQNSQGGAGSEQASQKTHIAGN
                     "
BASE COUNT          396 a          729 c          746 g          435 t
ORIGIN      
        1 acgtgccagc ctctgggcac aggaactcgt gcaacagcct cgagagccag ccccattggc
       61 tcgggaacgc gaggacgtgg cgcgggcacg cattctgctg gcccaggcga agccgactga
      121 ggcactcgcg ctcctggagc cgctgcgagg gggcgcgcag cggcagggac gctggaacca
      181 tgtgatcgag atgcaggtct tgcgggccct ggctcattcc atgggcgacc aggagcaaga
      241 ggccctggcc gtccttgccc aggcagtgca attggctgaa caggagggct acgtccgcat
      301 ctttgtagac gaaggcgctc cgatggctcg aatgctctcc cggttgaggg agcaggaaga
      361 caggcaggtg ccgaccgggt atgtggatac gctgctcgcc gcttttggcg aggtgggcgg
      421 ggtgcacgac gacgcaaccg gcgccggacc tctctgagca accgcgaact ggaagtgccc
      481 cggttgatgg cacgtggtgc tgcaccagca ggtggcgcgc cttaggggcg ttcgaggaaa
      541 cgagcgttgg caagtaagga aaggacacaa ccatggaaca acggctcgag tcagacctgg
      601 tcaagcgtct ggagcaactg ggaatcgaag tagacgtctc gcccacagga accacgctca
      661 cgtacgagca gatctacacc agtggcccca cgcttgagga ggcgttactc acgtttttgg
      721 gcgaggcttt gagcaggtac gaggccttgc gcacatggaa agctagtatg catgattgct
      781 agtttcctgc aatgtgcgtc ttctgggatg cttgctcgct acctgcaccg ccctggctgt
      841 tctgtccccc atgcacgatg gcaagcacct ggtcgcgctc gaggacctct tcttgttgca
      901 gcgcctgtgc caggcggtcc agggcctcgc gatgctcggt caagacgctg cgcgcctgct
      961 gatgggcttg cgtcatcagc tgctcgacct cagcatccag gcgttctgct gtcacctccg
     1021 agtacacacg ctcgtgacct tcgagcggtt gctggctgag ggaaggactt ccatccgaca
     1081 gggcataaac caccgggcca gtgtgctcgc ccattcccca ctcgctcacc atacgccgtg
     1141 ccagctgagt agcttccttg aggtcgttct ccgcgcctgt ggtgatatct cccagcgcca
     1201 gttcctctgc gctacggcca cccagggcca ccgccagccg ctcgagcagg tagctgcgcg
     1261 ggaggttgcg ccggtcatcg tctggccgga actgcgtgac ccccagcgaa cgcccgcgtg
     1321 gcacgatggt cactttgtag atgggatcga catcgggcaa caccgccgcc accagcgcgt
     1381 gtcccccttc atgataggcc acggtccggc gctcccgttc atccatcaag gcttcccgtt
     1441 tgccgcccag caggagtttg tcgagcgcat gctcgaagtc ctggcggctc acctggcgac
     1501 tgtctcgccg tgcggccgcc agcgccgcct cgttcaccag gttttccagg tcggctcccg
     1561 agaagccagg ggtcacccgt gccaggtccg ccaggttgac ttcaggcgcc agggggacgt
     1621 tgcgcgtatg gatgcgcagg atggcttcgc ggccacgtcg ttccggtagg tcgatcgtga
     1681 tctgccggtc gaagcgtccc ggtctcagca gggcgggatc gagcacgtca ggccggttgg
     1741 tggccgccag cacgaccacc gcctgagtgg gctcgaagcc atccatctcc accagcaatt
     1801 gctccagcgt ctgttcccgc tcatcattgc tggcaaaggc cgaggcgcca ctgcgcgtgc
     1861 gtccaatcgc gtcgatttca tcgatgaaga tgatgcacgg cgcgttcttc ttggcctgct
     1921 caaagagatc gcgcacgcgg ctggctccta ccccgacgaa gagttcgacg aagccggtgc
     1981 cgctagcgct atagaacggg acgcctgcct cgcctgcgac ggcgcgagcg agcagggttt
     2041 tgccggttcc cggtgggccg accagcagca cgcctttggg aatatgggct ccgatattgg
     2101 cgtagcgctg cggctcgcgt aggaactcga tttcctcttg caggtcggcc ttggcttcgt
     2161 cggcgcctgc cacatcggcg aaggtggtgg agggacgctc ttcgttgtag agtttggcac
     2221 ggctgcgccc aacgccaaag atgcctcctc cttgctgcga ctggcggaag ctgcgcaggg
     2281 ccatgtagcc taagtagatc agcagc
//
LOCUS       DASZAK010001350         6277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_69519, whole genome shotgun sequence.
ACCESSION   DASZAK010001350 DASZAK010000000
VERSION     DASZAK010001350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69519"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2174
                     /locus_tag="VGT44_23635"
     CDS             <1..2174
                     /locus_tag="VGT44_23635"
                     /inference="COORDINATES: protein motif:HMM:NF025011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HEV2583867.1"
                     /translation="GWLQNMGRLAQDMAQPIAARFAWEQLSLIERKVLHHALTLATTD
                     GVMRDLLFKLTHLSAEEFADAVVTLQQHLLMVEEQVLVKTGRAVTGAAAAKKLPSELT
                     IKLGVPKEILPVLVQVEREIYTPKQDRSNLKLEQILATIDVNKVYQIGTRYGFMLNDY
                     FTRTDPRARLAGQLVQPDVLYYAWDQLKGTARKLCKWIVEAGGSVSMNAAREFSGYDN
                     PTLAASITVLEEYALVFDTFVEHERRLFIPRELLKNLKKVIEQGDTGEAPDEVGLVPL
                     ERPPVAIHQGGAPALYDLTMIIGASFQQNMEPTQAGYIPKRVVNKLAPQMQMKPRLAT
                     YEQDNLNVDMLYTIASELNLLKLSRSEEASKPRYVEAKALREWSSKSEQEMAHDLLEY
                     WPKSRQWVDIAGVNYQSQQYDSLYYMNFESGRRELLAYLRTCEPGRWYSVSSLLKTIK
                     HEKPYLLRNQSYHTGVTGYRNAKTVLMKWFEVDGEILTGLLSSSLYEMGIVAVGFEQH
                     SSNGSQPANPVAFMVTELGAAALGSKKDAKRVAEEAADYAGGHERKRALIVQPNFELM
                     LLQPDMPTLYRLLPFAQIVQVGMVSRLNLTKTSLLRGLEQGRNIDQILKILTECSQNE
                     LPQNVVYTLKDWARAYKEVSISQVYLLETPSEVMGDDLAAMEKLKNYGLRRLGPCTFI
                     TGNDTNIQELRRLLEKEGIVTRAHGEFLTQKASSPGYSRWR"
     gene            complement(2193..2558)
                     /locus_tag="VGT44_23640"
     CDS             complement(2193..2558)
                     /locus_tag="VGT44_23640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583868.1"
                     /translation="MTTGPGREIAQTAQRSSDAKTRPYLPTFPTTSALENDDQVRRFE
                     LLRLAFFVLKLRKLEQRLHTITQESQETYLEHELTEQRNLLTYRCNLLHHVIFQQVLT
                     LIKLNARDQAMQIISTCQK"
     gene            complement(2720..3559)
                     /locus_tag="VGT44_23645"
     CDS             complement(2720..3559)
                     /locus_tag="VGT44_23645"
                     /inference="COORDINATES: protein motif:HMM:NF014881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HEV2583869.1"
                     /translation="MSAIIFDSKPLVAHMTAQLREQAVLFRKQHRRSARLAQIIAGRD
                     GAAEVYSRQIVKACQRIGLGCSTLAYPFEIEEEELRAEVARLSDDPLTDGVLLLLPLP
                     AHIRQQVVTEVLRPEKDVDGLGPRNAGNLLLGFPNFIPSTADVIRTILEDAGISVAGR
                     NAVVVGRSNIGGKPTALVLMRADATVTICHSHTHDLADLTRSADILVVSIGRPASITA
                     EMVKPGAVVLDAGINPVNGTVVGDVDFEHVRQVASFLTPVPGGLGPLTHLTLIRHTLM
                     GPQ"
     gene            3855..4346
                     /locus_tag="VGT44_23650"
     CDS             3855..4346
                     /locus_tag="VGT44_23650"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEV2583870.1"
                     /translation="MAADSSFDIVSQFDEQELVNALDQTRREVHTRFDLKDTKTEITQ
                     TKDSITITTDSSMTLKNVRDILETKAIRRQLPLKIFKPGNEENASGGKVRQVIALQQG
                     ISQELAKDISKQIRDAFPKVRPQIQGDAIRVTAKSRDDLQAVIAFLKAKDYPVALQFI
                     NYR"
     gene            4524..>6277
                     /locus_tag="VGT44_23655"
     CDS             4524..>6277
                     /locus_tag="VGT44_23655"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2583871.1"
                     /translation="MPGNRQVFSAAMNAADRLRWESQWTEAAKEYQRALAEFPDDTGA
                     RGGLGFCYMQTKQWKPALEEYEQVLKQEPSNIIPLSKTAELYVILDRRDDAYKAYLHL
                     ADLYSQAGQGPRAEAMWQKAAQLSATSPEPHERLAIYYASKKDYAMMVQERLIAAQGY
                     LLQNDNVSAQMQCEEVLLVDAANAQAQQMLSQLNGRARPAQTSPATAEESAVDMVSLS
                     NISGGNTGMMGNMGSAGNYNGGNVPGPALAAGTAGVTPPTPRKRITAGQVAGVLRQAQ
                     TFQSQGRFTDAIDLCEQIIESGFDRPDARYFLGWLYQEQRRWEDAIRQFLALLNDPDY
                     ALSCFYALGQCYRAKGDLSTAAKNFDEAVDRVNLDALTTEESDQLVQLCQEAAEAHRM
                     LGEQMQALNIYNALLGFLRNRGWSDKVAQVEFMLKQLQNTPAPQPSSTPLPPATQGNA
                     TRVLPPEQMTMGQSGQPQPYAPVQPGAANGQDDATVVFSRGAKPPSAPLPPTPSNALG
                     ELPGWLTGILNEADRTQMANKLQTPPAPPAQPSIINQPTMAQSPIANQPTMAQPQVPA
                     INAPEVSATWLTDGAPPT"
BASE COUNT         1428 a         1652 c         1955 g         1242 t
ORIGIN      
        1 agggctggtt gcagaacatg gggcggctgg cgcaggatat ggcgcagccg attgccgcgc
       61 gtttcgcctg ggagcagctc tcgctcatcg aacgcaaggt gctgcaccac gcgctgacgc
      121 tggccactac cgatggcgtg atgcgcgact tgctcttcaa gctcacgcat ttgagcgcag
      181 aggagtttgc cgatgctgta gttacgctac aacagcatct gctgatggta gaagagcagg
      241 tgctggtgaa gacaggtaga gcggttactg gagcagcggc ggcgaaaaaa ctcccgtcag
      301 aattgacgat caagctgggt gtgccgaaag agattttgcc tgtgctggtg caggtcgaac
      361 gtgaaatcta tactcccaag caagaccgct cgaacttgaa gctcgagcag attctagcaa
      421 ctatcgatgt caacaaggtc taccagattg gcactcgcta cggcttcatg ctgaacgatt
      481 acttcacgcg caccgatcca cgcgcgcgac tggccgggca gcttgtgcag cccgatgtgc
      541 tgtactatgc ctgggatcag ctcaaaggca cggcgcgcaa gctgtgcaaa tggatcgtgg
      601 aggccggtgg gtccgtcagc atgaacgcgg cgcgggaatt tagcggctac gacaatccta
      661 ccctggcggc cagtatcacc gtgctggaag aatatgcgct cgtctttgat acattcgtgg
      721 agcatgagcg ccggctgttc atcccacgcg aactactcaa gaatttgaaa aaagtgatcg
      781 agcaggggga tacgggcgag gcgcctgacg aggtggggct ggtacccctg gagaggccgc
      841 ccgtcgctat ccaccagggc ggcgcgccgg cgctgtatga cctgacgatg attatcggcg
      901 cgtcgttcca gcaaaatatg gagccgaccc aggcgggtta cattccaaaa cgtgtcgtga
      961 acaagctggc gccccagatg cagatgaagc cgcgcctggc gacctacgag caggataacc
     1021 tgaacgttga tatgctgtac acgattgcct cggaactgaa cttgctgaaa ctcagccgct
     1081 cggaagaagc atcaaagccg cgctatgtcg aggccaaagc gctgcgcgaa tggtcgtcga
     1141 agagcgagca agaaatggcg catgatctgc tggaatactg gccgaagagc aggcagtggg
     1201 tggatattgc cggagtcaat tatcaaagcc agcagtacga ttcgctgtac tacatgaact
     1261 tcgagtcggg gcgcagagaa ctgctggcct acctgcgcac gtgcgagccg ggcaggtggt
     1321 atagcgtctc atcgttgctc aagaccatca agcatgagaa gccgtacctg ctgcgcaacc
     1381 agtcctacca tacgggggtg acgggctatc gcaacgcgaa gacagtgctg atgaagtggt
     1441 tcgaggttga tggggaaatc ctgacgggct tgctctcctc atcgctgtac gagatgggta
     1501 tcgtggcagt gggctttgag caacattcgt caaatgggag ccaacccgcc aatcccgtcg
     1561 ccttcatggt gacggaactc ggcgcagccg cgcttggttc aaaaaaggat gcaaagcgcg
     1621 tagcggagga agcggcggac tacgcgggcg gacacgaacg caaacgcgcg ctgatcgtgc
     1681 agccgaattt cgagttgatg ctgctacaac cggatatgcc gacgctctat cgcctgctgc
     1741 cctttgcgca gattgtgcag gttggcatgg tcagtcgcct gaacctgacg aagacaagcc
     1801 tgctgcgcgg cctggagcag ggtcgcaata tcgaccagat tctgaaaatc ttgacggaat
     1861 gcagccagaa tgaactgcct cagaacgtgg tctacaccct taaggattgg gcgcgagcgt
     1921 acaaggaagt gagcatttcg caggtatacc tgctggagac gcccagcgaa gtgatgggag
     1981 atgacctggc ggctatggag aagctgaaga attacggcct gcgcaggctc ggtccctgta
     2041 cctttatcac gggcaacgat accaatatcc aggagctgcg gcgcttgctg gagaaagaag
     2101 gtatcgttac tcgcgcgcat ggcgaattct tgacgcaaaa ggcaagttcg cctgggtata
     2161 gccggtggcg atagcccatc accccaaatg aatcattttt ggcaggtgga gatgatctgc
     2221 atggcctggt cgcgggcgtt gagcttgatg agcgtcaaca cttgctggaa aataacatgg
     2281 tggagcaagt tgcagcgata ggtcagcaag ttgcgctgtt cggttagctc gtgttccaga
     2341 taggtttctt gtgattcctg ggtgatggtg tgcaggcgct gttccagttt gcgcagtttc
     2401 aggacaaaaa aggccagccg caggagttca aagcgtcgaa cctggtcgtc attctctaac
     2461 gcgcttgtgg tggggaaggt aggaagatag ggccgcgtct tcgcatcgga acttctttgt
     2521 gccgtttgtg ctatttctct gcccgggccg gtagtcatat cgtactcctc tggtctctcg
     2581 ttgacgtgcg gcatccatgc gcgtttatca tttcctggct gcaattctac ctgtcgcggg
     2641 ggtgggatga agtggagaac aggtgggaat atggttaaga aacaagcagg catcgttgac
     2701 tttttataac atggattagt tattgcggtc ccatcagggt gtggcggatg agggtgagat
     2761 gcgtcagggg gccgagtccg ccggggacag gcgtcagaaa tgaggcaacc tggcgcacgt
     2821 gctcgaaatc gacatctcca acgaccgtgc cgttgacggg attgatgccc gcgtccagca
     2881 cgaccgcgcc gggcttgacc atttcggcgg taatgctggc gggccgtccg atactgacga
     2941 ccaggatatc ggcgctgcgc gtcaggtcgg caagatcgtg cgtgtgcgag tggcagatcg
     3001 tgacggttgc gtcggcacgc atcaggacga gcgcggtggg tttgccgcca atgttgctgc
     3061 ggcccacgac gacggcgttg cgcccggcta cggagatgcc cgcgtcttcc aggatggtgc
     3121 ggatcacatc ggcggtcgaa gggataaagt tggggaagcc cagcagcaga ttgccggcgt
     3181 tgcgcggccc caggccatcg acatccttct ccgggcgcag cacctccgtg acgacctgct
     3241 ggcgaatatg ggcgggcagc ggcaagagca gcaagacgcc atcggtgagc gggtcatcgc
     3301 tcaaccgggc cacctctgcg cgcagttctt cctcctcgat ctcgaagggg taggccagtg
     3361 tggagcagcc caggccgatg cgctggcaag ccttcacgat ctgcctgctg tagacctcgg
     3421 cggccccgtc gcgcccggcg atgatttgcg ccaggcgcgc cgaacgacga tgctgtttac
     3481 ggaagaggac ggcctgctct cgcaattggg ccgtcatatg cgcaacgagc ggcttgctat
     3541 cgaaaataat ggcggacatg cgtgtatctc tctgtaaaaa agggcgaccg ccagggtgcg
     3601 cccttacaat gaccacgatg tggccaggcg catgtggcgt atcattgtaa gggcgcaccc
     3661 tggcggtcgc ccttgcggtt gtagacatgc gtgtatgata tcacatcccc ccatttgggg
     3721 gataggagtt gccaaatgca taaagaatgt attaagattg gcacgttaac caacaccatg
     3781 acatttgttg aaatatgtgg atatgcttaa actagccatt cggtaccata atagattgct
     3841 gagagaggaa tttcatggca gctgatagtt catttgatat agtatcgcag ttcgatgagc
     3901 aggagctggt caacgcgctc gatcagacgc ggcgcgaggt gcatacgcgc tttgacctga
     3961 aagacacgaa aacggagatc acgcagacga aggatagcat caccattacg accgatagca
     4021 gtatgacgct caaaaatgtg cgcgacattc ttgagacgaa agcgattcgc cgccagttgc
     4081 cgttgaaaat ttttaagccg ggcaacgaag agaacgcttc gggcggcaag gtgcgccagg
     4141 tcatcgcctt acagcagggc atttcgcagg aactggcgaa ggatatcagc aagcagatcc
     4201 gcgatgcttt tccgaaggtg cgaccgcaga ttcagggcga tgccattcgc gtcacggcca
     4261 aaagccgcga tgatttgcag gcggtaatcg cgtttctgaa agcgaaagat tacccggttg
     4321 ccttgcagtt catcaattac cggtgataat gagacggtcg cgttcattcg gatgatgggt
     4381 actttagtac cacccagaca gcatcattgc tcacgaaaat cgtatgtata gtacgctggt
     4441 ggtaactgga tgcagtatcg tcaggttggc tggcgagaac gcgagcgtat ttcaccggtg
     4501 cctgctaagg aagagaatct ttcatgcctg ggaacagaca ggttttcagc gcagctatga
     4561 acgctgctga tcgcttgcgt tgggaaagcc agtggacaga agcggcgaaa gagtaccagc
     4621 gagcactggc cgagttccca gacgatacgg gagcgcgagg cggtctaggg ttttgctaca
     4681 tgcaaaccaa gcaatggaaa ccggcgcttg aggagtatga gcaggtcctg aaacaggagc
     4741 cgagcaatat catcccgttg agcaaaaccg ccgagctgta cgtgattctg gacaggcggg
     4801 acgatgcgta caaggcgtac ctgcacctgg ccgatctgta ttcgcaggcg ggacaggggc
     4861 cgcgcgccga agcgatgtgg cagaaggccg cgcaattgtc tgcgacctcg ccggagccac
     4921 acgaacggct ggcgatctac tacgcgagca aaaaagatta tgcgatgatg gtccaggagc
     4981 gcttgattgc ggctcagggc tatctcttac aaaacgataa cgtgtcggcg caaatgcagt
     5041 gtgaagaagt gctgctcgtc gatgcagcca acgcacaggc tcaacagatg ctttcgcagc
     5101 tgaatgggcg ggcgcggcct gcgcagactt caccggcaac ggcggaagaa agcgcagtgg
     5161 atatggtctc tttgagcaac ataagcggag ggaatactgg catgatgggt aatatgggta
     5221 gcgcgggcaa ctataatggg ggaaacgttc cgggtccggc gctcgcagca ggaacagccg
     5281 gtgtaacacc accgacccca cgtaaacgta ttacagctgg ccaggtggca ggtgtgctgc
     5341 gccaggcgca aacctttcag agccaggggc gtttcaccga tgctatcgac ctctgcgagc
     5401 agatcatcga aagcggcttt gatcgacccg acgcccgcta ctttctcggc tggctctacc
     5461 aggaacaacg gcgctgggaa gacgccattc gccagttcct ggcgttgttg aatgatcccg
     5521 actatgcgct ctcttgcttc tatgcgctcg gccagtgcta tcgagcgaag ggtgacctga
     5581 gtaccgccgc caagaatttc gatgaggcgg ttgaccgcgt caaccttgat gccctgacga
     5641 cggaggaatc ggaccagctg gtgcagttgt gccaggaggc agccgaggcg caccggatgc
     5701 tgggtgagca gatgcaggcg ttgaacattt ataatgccct gctcggcttc ttgcgcaatc
     5761 gtgggtggag cgataaggtg gcgcaggtag aattcatgct caaacagttg cagaatacac
     5821 cggcgcctca accgtcgtcg actccattgc cgcccgcgac tcagggcaat gcaacgcgcg
     5881 tcctgccgcc ggagcaaatg acgatggggc aaagcggaca accacagccc tacgcaccgg
     5941 tgcagccggg cgcggccaac ggacaggacg acgcgacggt agttttttcc agaggggcaa
     6001 agccgccatc ggcgccgctg ccgccaacgc cttccaacgc gctcggcgag ttgcccggct
     6061 ggttaacggg tattctgaat gaagctgaca ggacgcagat ggcgaacaag ttgcagacgc
     6121 caccagctcc gccagcccag ccttctatca tcaatcagcc aacaatggcg caatcgccga
     6181 ttgctaatca gccgactatg gcacagccgc aagtgcctgc tataaatgcc cccgaagtct
     6241 cggccacgtg gctgacggat ggcgcgccgc ccacaca
//
LOCUS       DASZAK010001351         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_176688, whole genome shotgun sequence.
ACCESSION   DASZAK010001351 DASZAK010000000
VERSION     DASZAK010001351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176688"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1423
                     /locus_tag="VGT44_23660"
     CDS             <1..1423
                     /locus_tag="VGT44_23660"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV2583872.1"
                     /translation="HITSNLTRQFETNPEQLREVLHYIIAVGSSLRLQIDVDTLLREI
                     AVAACKALRFRYSALYLTDDQGYYRVRATAGISERDEALLHDLPIPDHIVAMLINDDY
                     RISDSYFFPAEAPIWQNQEFTSYFVIVDEKEQLTPLTPLRTPPLSKHLWLQEDLLVVP
                     LVSGDNTLLGFLTPDAPLSGRRPNVETMALFELFMNQAAVAIEGARLHADLRQALKQA
                     QESERIKNQFLMTASHELRTPLTAVQGYVELLSNFRETLDDETQVRFLQNARRACDEL
                     VLLLGNVMDASRIDQDRVLLKLAPMKVSQAVETILEILEPIIAREKRKVGVHIPEEYV
                     AWVDELRLRQVLLNLVGNALKYTPEGTPLELRAERLTWTALVERIPACSPQLPIPASG
                     AFVVIAVRDWGPGIAPEDQAQLFSKFVRLNEAINSIQRGAGLGLYLCRQLTEAMGGYT
                     WLESDGIAGQGSAFYAAFPLYKE"
     gene            1595..1951
                     /locus_tag="VGT44_23665"
     CDS             1595..1951
                     /locus_tag="VGT44_23665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583873.1"
                     /translation="MNLFEQLQVVRERLEGIGANDDSMDLVEKLLRKAEPARSDKTSI
                     TQVQVLRHMLRMPEVIDNYNVYNDLQELLSERDEFEVSAREDAAPEAYVDTERHPRPK
                     SFYKAKKEKEKEKKKS"
     gene            complement(1948..2691)
                     /locus_tag="VGT44_23670"
     CDS             complement(1948..2691)
                     /locus_tag="VGT44_23670"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEV2583874.1"
                     /translation="MGIFSRLATFFHIKINSALDKAEDPGQVLDYSYSKQLEQLQQLR
                     RSIAEVVTNEKRLEMQQSQQLEKVNRLDGQAMQALQANREDLARMALQRKEAMLVEIN
                     NYEQQIAQLRAQEERLVSMERTLSARIDAFRTQKEMVKAQYNAAQAQVKINETVTGIS
                     QEMNEMNMAMQRTQDKVLTMQARAEAMNTLIEQGTLGEQGLLGSVDTLERELHQIASE
                     QNVEAQLQAMKQQMQLGGPNAQQQLPPGS"
     gene            2815..2889
                     /locus_tag="VGT44_23675"
     tRNA            2815..2889
                     /locus_tag="VGT44_23675"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2847..2849,aa:Gly,seq:ccc)
     gene            complement(2946..>3683)
                     /locus_tag="VGT44_23680"
     CDS             complement(2946..>3683)
                     /locus_tag="VGT44_23680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV2583875.1"
                     /translation="AWRMFEPGRGVFDNAGDDTNEATTAHVTIGNDFWASVKKLRSVS
                     TYWTLVGALVFSFFTIGGTSFWLPTYFVGTFHLSVGQAGTISGAVLVGSGLIGTVAGG
                     WLADFAQRRRPEGRLLIATIGFLVGAPLVLIALLIHTLPLFIAVFVIAAISLNFCTGP
                     LNAVIQDVISPALRATAIGLALLLAHLLGDAAAPSVIGAIANNSSLGFALTVTAPTFL
                     FLAGLVCLLGLRTVARDMHRMQAQFRE"
BASE COUNT          818 a         1037 c          991 g          837 t
ORIGIN      
        1 acacatcact tcaaacctga cccggcaatt tgaaacgaat cccgagcaac tgcgtgaagt
       61 attgcactat attattgcgg ttggctcttc gctgcgcttg cagatcgacg tggatacctt
      121 gctgcgggaa atagcggtcg ctgcctgtaa agcgctgcgc tttcgctact cggctctcta
      181 tctgacggac gatcaagggt attatcgagt gagagctact gccggcataa gcgagcggga
      241 tgaagccttg ctgcatgatc tgccgatccc cgatcatatt gttgccatgc tcatcaatga
      301 cgactatcgt atcagtgact cgtatttctt cccggcagaa gcccccatct ggcaaaatca
      361 agaatttacc agctatttcg tgattgttga tgagaaagaa cagttgacac cgctgactcc
      421 cctgcgcact cctcccctct cgaagcacct ctggcttcaa gaggacttgc tggtcgtgcc
      481 gctagtcagt ggcgacaata cgttgctcgg ctttctgaca cccgatgccc ccttgagcgg
      541 tcggcgtccg aatgttgaga cgatggcgct ctttgaactc ttcatgaacc aggctgcggt
      601 tgccatcgaa ggcgcgcgtc tccatgccga cctgcgccag gcattgaagc aggcccagga
      661 gtctgaacgc atcaaaaacc agtttttaat gaccgcctcg cacgagctgc gcaccccgtt
      721 gaccgccgtg cagggctatg tcgaactcct gtcgaatttc cgcgagacgt tggatgacga
      781 gacccaggta cgcttcctgc aaaatgcgcg ccgcgcctgc gatgaactgg tgttgttgct
      841 tggcaatgtc atggacgcca gccgcataga ccaggatcgt gtgctgctca aactggctcc
      901 aatgaaggta tcccaggccg tcgagactat tcttgaaatc ctggagccga tcattgcgcg
      961 cgagaaacgt aaggtgggcg tccacattcc cgaagaatat gtcgcctggg tcgatgaact
     1021 gcgcctgcgc caggtcttac tgaacctggt tggcaatgcc ttgaaatata cgccggaagg
     1081 gacacccctg gagctgcgcg ccgaacgcct gacctggacg gcgctggtcg agcgtattcc
     1141 cgcgtgttcg ccgcagctcc ccattcccgc atcgggcgca ttcgtcgtca tcgctgttcg
     1201 cgactggggg ccgggcatcg ctcctgaaga ccaggcgcaa ttgttcagca aatttgtgcg
     1261 tttaaacgag gccatcaata gtatacagcg cggcgcgggc ctgggactct atctctgtcg
     1321 ccagcttacc gaggccatgg gcggctacac ctggctggag agcgacggga ttgccggtca
     1381 gggcagcgcc ttctacgccg ccttcccact ttacaaagaa taatctatag ggtcaaggcg
     1441 atgggctgta ggagccaggg cgatggccgg catcgcccct atcctctccg attcccccat
     1501 gtgaccatca cacatcgatt tgacgggctg catcggctat gttaccatag cggcagggtt
     1561 ctgttgccca cacgcggaag gagtactgaa cgcaatgaat ttatttgaac aattacaggt
     1621 agtacgggag cgtctggagg gtattggcgc gaacgatgat agtatggacc ttgtcgagaa
     1681 gctcttgcgc aaagcggagc cagccaggag cgataaaacg agcattacgc aggtgcaggt
     1741 cttgcgccac atgctacgta tgcctgaagt gatcgataat tataacgtct acaatgactt
     1801 gcaggagctg ttgagcgaac gagacgaatt cgaggtaagc gcacgcgaag acgctgctcc
     1861 ggaagcctat gtcgatacgg agcgccatcc tcgacccaaa tctttctata aggccaagaa
     1921 ggaaaaagag aaagagaaaa agaagagcta gctccctggc ggcagttgtt gctgagcgtt
     1981 cgggccgccc agctgcatct gctgcttcat ggcctgtagc tgcgcttcaa cattttgctc
     2041 cgaggcgatc tgatgcagtt ctcgttccag cgtatcgacg gagccgagca gtccctgctc
     2101 gccaagcgtc ccctgctcga tcaacgtatt catggcttcg gcacgagctt gcatggtcaa
     2161 taccttatcc tgggtgcgct gcattgccat attcatctcg ttcatctcct gtgaaatgcc
     2221 cgtcaccgtc tcgttgatct tcacctgggc ctgggccgcg ttatactgcg ccttgaccat
     2281 ctctttttgc gtgcggaacg cgtcgatgcg cgccgagagg gtgcgctcca tcgagaccag
     2341 ccgctcctct tgagcgcgca gttgggcgat ttgctgctcg tagttgttga tctcgactaa
     2401 catcgcttct ttacgctgca aggccatgcg cgccaggtcc tcgcggttgg cttgcaaggc
     2461 ttgcattgcc tgcccgtcga gccggttgac cttctcaagc tgctgcgact gctgcatctc
     2521 cagacgtttt tcgttcgtga ccacctcggc gatcgaccgg cgcagttgtt gcagctgctc
     2581 taattgcttg ctataggagt aatccagtac ctgccctggg tcttcagcct tatccagcgc
     2641 ggaattgatt ttgatgtgaa aaaatgttgc gagtcgtgaa aagatgccca taaagacctc
     2701 catagatgtc gttggcccga ccacatcata tcatatcaag agtccaaaat gcttgacttt
     2761 tcccatccca tcgtctataa tcccccctat cggcggcctg tacagggctg tcgcgcgggt
     2821 ttggtgtagt ggtaacatgc gactttccca aagttgagac cacgggttcg aatcccgtaa
     2881 cccgctccac gaatggcttc tatcattgag atagaagcca tgtttgttct ctgcaagctt
     2941 ttttcttact cgcgaaactg cgcctgcata cgatgcatat cgcgcgcaac cgttctcaat
     3001 ccaagcagac aaaccagtcc cgcgaggaac aggaaggtgg gcgccgtaac ggtaagcgca
     3061 aaaccaagtg aagaattatt tgctattgcg cctattacgg atggagctgc cgcgtctcca
     3121 agtagatgcg caagcagcaa agccaggcca attgcggtgg ctcgcaaagc gggagaaatt
     3181 acatcctgga tcactgcatt caatggcccg gtacagaaat tgagcgagat ggcagctatg
     3241 acgaacaccg cgataaaaag tggcagggtg tggatcaata gcgcgatcag caccaggggc
     3301 gcgcccacta gaaaaccgat agttgcgatc agcaaacgtc cttcgggccg gcggcgctgc
     3361 gcaaagtcag ccaaccagcc gccagcaact gttccaatca gtccgctgcc caccagcaca
     3421 gccccggaga tggtccctgc ttgacctacg ctaaggtgaa acgtgcctac gaagtacgtt
     3481 ggaagccaga atgaagtgcc gccaattgtg aagaaactaa acacgagcgc gcctactaat
     3541 gtccagtatg tcgaaacaga gcgtagtttt ttcacggatg cccagaaatc atttcctata
     3601 gtcacatgtg ctgtagtggc ttcgtttgtg tcgtccccgg cattatcgaa tacgccacgt
     3661 ccgggttcga acatacgcca cgc
//
LOCUS       DASZAK010001352         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_139674, whole genome shotgun sequence.
ACCESSION   DASZAK010001352 DASZAK010000000
VERSION     DASZAK010001352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139674"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..1093
                     /locus_tag="VGT44_23685"
     CDS             80..1093
                     /locus_tag="VGT44_23685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEV2583876.1"
                     /translation="MPRRITAIILMVTAIALVVAAILLAFTQVGTRFFGGPARATPVG
                     IIPSPTFLPFTPTPSPSPTPVLTARGQPGPGAITANEAILVDEDSGNILYDLNGERPQ
                     PMASTTKIMTALLAMQAMDLTTLVRIQQDALNEVVNNNGSTAGLRVGDELTLRDLLYG
                     LMLPSGDDAAIAIADAVGGAVPNFVRMMNLYAARLGLFQTHYYNPDGLTYYDANGNAI
                     SGHYTTAYDLARLARYAMSIPLFAQLVSTKHYVVPPTSLHHAYNWTSINQLLTSYPGA
                     TGIKTGYTLEAGGCLVFSATRSGHHLLGVVLHSIDEPHRFSDATTLLNWGFGLPVRVA
                     STT"
     gene            complement(1101..>2142)
                     /locus_tag="VGT44_23690"
     CDS             complement(1101..>2142)
                     /locus_tag="VGT44_23690"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV2583877.1"
                     /translation="LPRFQSDFTEKALVEYAQKRLSERLKSPFRELTRYGVLLRRYDL
                     PVLQAVFPEYFQNRDALDIFHQLNDYPYVESLGDQYHAFHDLLREIQAAEIRKQQPSE
                     WQTYHRRALEYLAQNDPDSPDRYYHMIALDEEQGISDWWDAVTNPRHYGSYLGALFQA
                     ANDVTLALSPTQDAHRLYRTGVNNYMGARMSDALSCYQEAFSLYRQVGSSLGEANVRK
                     AIGDVQQFRDEREAALASYEAALSLFRQVGSSLGEANCFQSLGHLALEQDEHQRALEF
                     YNDAYRLYCQIEDRYSQTALLFYRSFVYEAMEQFPQAIHDMEQALALAQQLSLSQVEA
                     CQERLDELRGQA"
BASE COUNT          457 a          655 c          608 g          422 t
ORIGIN      
        1 agtggggtgg atgtggggtg gagacccttg cggtcgccct cgttccaaac gtgagtatta
       61 cccttctaga ggatagaaca tgccgcgtcg catcaccgcc atcatactaa tggtcaccgc
      121 catcgccctg gttgtcgcgg cgatcttgct ggcctttacg caggtaggga cacggttctt
      181 cggcggaccg gcgcgggcga cgccggtggg catcatccca tcgccgacgt ttctgccgtt
      241 cacgcccacg ccgtcgccat caccgacacc cgtactgacg gcgcgcgggc agccgggacc
      301 gggggcgatc accgcgaacg aagccatcct ggtggacgag gatagcggca atatcctcta
      361 cgatttgaac ggggagcgac cgcagccgat ggccagcacg accaagatca tgaccgcgct
      421 gctggccatg caggcgatgg acctgactac gctggtgcgc atccagcagg acgcgctcaa
      481 cgaagtggtc aacaacaacg gcagcacggc gggcctgcgc gtgggggatg agctgacgct
      541 gcgcgacctg ctctacgggc tgatgctgcc gtccggcgac gacgcggcta tagcaatcgc
      601 cgacgcggtg ggcggcgccg tccccaattt cgtcaggatg atgaacctct atgccgcgcg
      661 cctgggcctc ttccagactc actactacaa ccccgacggc ctgacctatt acgatgcgaa
      721 cggcaacgcc atatcaggcc actacacgac ggcctatgac ctggcgcggc tggcgcgcta
      781 cgccatgtcc atcccgctct tcgcgcagct cgtcagcaca aaacactacg tcgttccccc
      841 caccagcctg catcacgcct acaactggac gagcatcaac cagttgctga ccagctaccc
      901 cggcgcgacg ggcatcaaga cgggctacac gctcgaagcg ggcggctgcc tggtcttctc
      961 cgccacgcgc agcggccacc acctgctcgg tgtggtgctg cacagcatcg acgaacccca
     1021 ccgcttcagc gacgcgacga cgctactgaa ctggggattt gggctgcctg ttagagtggc
     1081 gagtacaacc tgaccgccaa tcacgcctgc ccccgcagct catcaagacg ctcttgacag
     1141 gcttctactt gagaaagact cagttgttgg gcaagggcca gcgcctgctc catatcgtgt
     1201 attgcctgtg gaaactgctc cattgcctcg tagacaaaag agcggtaaaa aagcaaggcg
     1261 gtctggctgt aacgatcttc aatctgacaa tagagtcgat aagcatcatt ataaaattct
     1321 aaagctcttt ggtgttcatc ctgttctaaa gcaagatgtc ctaacgactg gaaacaattg
     1381 gcttccccta agctcgaacc cacctgccgg aacaggctca aggctgcctc atacgacgcc
     1441 agcgccgcct cccgctcatc gcggaactgc tgcacgtctc cgatggcttt ccgcacgttg
     1501 gcttccccta agctcgaacc cacctgccgg tagagcgaaa aggcttcctg gtaacaagac
     1561 aaggcatcgg acattcgcgc gcccatataa ttattaaccc cggttctgta gagacgatgg
     1621 gcgtcctggg taggagaaag cgcgagcgtc acatcgttag cagcctgaaa cagagcgccg
     1681 agatagcttc cataatgccg gggattcgtg accgcgtccc accagtccga tatgccttgc
     1741 tcttcatcga gagctatcat gtgataatac ctgtctggcg aatctgggtc gttttgagcg
     1801 agatactcta aagcgcgccg gtggtaggtt tgccactccg atggctgctg tttgcgtatc
     1861 tcggcggcct gtatttctcg caacagatca tgaaaagcgt ggtactggtc tccaagtgac
     1921 tccacatacg gatagtcgtt gagttgatgg aagatatcga gggcgtctcg attctggaaa
     1981 tactctggaa acacggcttg cagcacaggc aggtcatagc ggcgcaggag tacgccgtag
     2041 cgtgtcagct cgcgaaaagg tgatttcaga cgctcgctca gacgcttctg ggcatattcg
     2101 acaagggctt tttcggtgaa atccgactgg aagcgcggca gg
//
LOCUS       DASZAK010001353         3498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_176823, whole genome shotgun sequence.
ACCESSION   DASZAK010001353 DASZAK010000000
VERSION     DASZAK010001353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176823"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..3317)
                     /locus_tag="VGT44_23695"
     CDS             complement(36..3317)
                     /locus_tag="VGT44_23695"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583878.1"
                     /translation="MANENGTVHRRSSNGTADEQSKQPAVNPALLQTLEWRCIGPFRG
                     GRVVAVAGDPVHAQVFYFGSTGGGVWKTTDGGIIWENVSDGYFKRASVGAIAVAPSDP
                     NVVYVGMGETTIRSNVSHGDGVYRSTDGGQTWTHLGLADTRNIAIVRVHPSNPDLVYV
                     AALGHAHGPNAERGLYRSKDGGSTWERILFRSEKAGAIDLSMDPHNPRILYAAIWEAH
                     RKPYTLVSGGEECGLFKSMDGGDSWKEITRNEGLPKGMLGKIGVAVSPAKENRVFAII
                     DAEDGALFRSDDGGEHWERMSEDRNLWARPWYYMHVIADPQNAETIWALNVQAWKSTD
                     GGKTFSEVQIPHGDHHDLWIDPLNPLRMIEGNDGGACVSFNGGASWSSIYNQPTAEFY
                     HVTTDTQVPYHVYGAQQDNSTICVPSSHPYGSITNAMTYEVGGGESGYIAVRPDDPNI
                     TYAGNYQGYLARADHRTLQERNIAVWPEVATGWGAIDQKYRFQWTSPILLSPHDPNVL
                     YTTGNYVFRSTDEGNSWEIISPDLTRNDVTKQEASGGPPTKDNTGAEYYCTIFAFAES
                     PIAPGLFWAGSDDGLVHISRDGGASWQNVTPVDLPEWALISIIEPSPHDPATAYIAAT
                     RWKWDDFHPYLYKTNDYGATWTTITGGIADNDFTRVIREDPARRGLLFAGTETGIHVS
                     FDDGARWQSLRANLPVVPIHDLVVKDSDLVVATHGRSFWILDDISPLRQVSEQAEQIA
                     SAPAFLFKPRPTLRLNMSYGYSHAAHPGNTFRDTENTQVVARRVERPGKVWLDRPLNA
                     GQNAPGGVIVYYTLAEKPEGEVKLTFLDAEGSEIKAFTSKAERKKAADPTDEEEEEEK
                     QDPNTPNDAGLNRFVWNMRYPDPRKVDGYVSSDGAMSGPAIIPGNYQVRLTVGDISIT
                     ESFEVRKAPYITATQADLEARLTLLLKLRDKLSETHDTINTLRNVRQQIDDWLKRTKE
                     LPAFESLERLGKSIKEQLKPIEDELIQSKAKTRQDTLNHPAKLNGKLAGMAYVLAGAQ
                     AAPTQQANALFDDLCRRIDAQAQRLREVIDGDVAAFNALMREQNVPALIPVATLDNKN
                     EKKNR"
BASE COUNT          643 a         1003 c         1086 g          766 t
ORIGIN      
        1 tggtaagggc gtacccttgt ggtcgccctt tcgtatcatc gatttttctt ctcgttttta
       61 ttatccagcg tagccaccgg aatcaacgcg ggcacattct gttcgcgcat caacgcattg
      121 aaggccgcta cgtcgccgtc gatgacctcg cgcaggcgct gagcctgggc atcgatgcgc
      181 cggcagagat cgtcgaagag ggcgttggcc tgctgcgtgg gcgcagcctg cgcaccggca
      241 agcacatagg ccatgccggc cagcttgccg ttcagtttgg caggatgatt gagcgtatcc
      301 tggcgcgttt tggccttcga ctggatcagt tcgtcctcga tgggcttgag ttgctccttg
      361 atcgacttgc ccaggcgttc cagcgattca aaggcgggca gttcctttgt gcgtttcagc
      421 cagtcgtcga tctgctgccg cacattgcgc aaggtattga tggtatcgtg cgtctccgag
      481 agcttatcgc gcagcttgag cagcagagtc agtcgcgcct ccaggtctgc ctgcgtagcc
      541 gtgatatacg gggctttgcg cacctcgaag gattcggtaa tgctgatgtc acctactgtc
      601 aaacgcacct ggtagttacc ggggatgatg gcagggccag acatcgcccc gtcggaggaa
      661 acgtatccat caactttgcg cgggtcggga tagcgcatgt tccagacgaa gcgattgaga
      721 cccgcatcat tgggcgtgtt ggggtcctgc ttctcctctt cctcttcttc gtcggtcgga
      781 tcggcggcct tcttgcgttc ggctttgctg gtaaacgctt tgatctcgct cccctccgcg
      841 tccaggaagg tcagcttcac ctcaccctcc ggtttctccg cgagcgtata gtagacgatg
      901 accccgccgg gcgcattctg ccccgcgttg agcggtcgat ccaaccagac cttgccgggg
      961 cgttcaacgc ggcgcgccac cacctgggta ttttcggtgt cgcggaaagt gttgcccgga
     1021 tgcgcggcgt gggaatagcc atagctcata ttcaaacgca aggttggacg cggtttgaag
     1081 agaaaggccg gagccgacgc gatctgctcc gcctgttcgc tgacctggcg caagggcgag
     1141 atatcgtcga gaatccagaa ggagcggcca tgcgtagcca caaccaggtc ggagtccttc
     1201 accaccaggt catgaatggg aacgaccggc aggttagcgc gcagcgattg ccagcgcgcg
     1261 ccgtcatcga aggagacatg gatgcccgtc tccgttcccg cgaagagcag gccgcgccgc
     1321 gccgggtctt cgcgaatgac gcgcgtgaag tcgttgtcgg cgatgccgcc ggtgatcgtc
     1381 gtccaggtcg cgccgtagtc gttggtctta tagaggtagg gatggaagtc gtcccatttc
     1441 cagcgtgtcg cggcgatata ggccgtcgcg ggatcgtgcg gcgacggctc gatgatgctg
     1501 atgagcgccc actccggcaa atcgacgggg gtgacgttct gccaggacgc gccgccatcg
     1561 cgcgagatat gcacgaggcc atcgtcggag ccagcccaga acaggccagg cgcaatcggc
     1621 gattcggcaa aggcgaagat ggtacagtaa tattccgctc cggtattatc cttcgtcggc
     1681 ggtccgccgg atgcctcctg cttcgtgacg tcgttgcgcg tcaggtcggg actgatgatc
     1741 tcccagctat tgccctcgtc ggtcgagcgg aagacatagt tgccggtggt atacagcacg
     1801 ttggggtcgt gcggcgaaag taggatgggg gacgtccact ggaagcggta tttctggtca
     1861 atcgcgcccc agccggtggc gacttctggc cagaccgcaa tgttgcgttc ctggagggtg
     1921 cgatgatcgg cgcgcgccag gtagccctgg tagttcccgg cgtaggtgat gttggggtcg
     1981 tcggggcgca ctgctatata gccgctttcg ccgccgccaa cctcgtaggt catggcattg
     2041 gtgatactgc cgtaggggtg gctggagggg acgcagatgg tcgagttgtc ttgctgcgcg
     2101 ccatagacgt gatagggaac ctgggtatcg gtagtaacgt ggtagaattc ggcggttggc
     2161 tggttatata tgctcgacca gctcgcgccg ccattgaaag agacgcaggc gccgccgtcg
     2221 ttgccttcga tcatacgcaa ggggttgagc gggtcgatcc acagatcgtg atgatcgcca
     2281 tgcggtatct gcacctcgga aaaagtcttt ccgccatcgg tggatttcca ggcttgcacg
     2341 ttgagcgccc agatcgtttc ggcattctgc ggatcggcaa tgacatgcat gtagtaccag
     2401 gggcgcgccc ataggttgcg gtcttcactc atgcgctccc agtgttcgcc gccgtcgtcg
     2461 gagcgaaaca acgcgccatc ttcagcgtcg atgatggcaa agacgcggtt ctctttggcg
     2521 ggcgagacgg ccacgccgat cttgcccagc atacccttgg gcagaccctc gttgcgcgta
     2581 atctccttcc agctatcgcc gccatccatc gatttgaaga ggccgcattc ctcaccgccg
     2641 ctgaccagtg tgtagggctt gcgatgcgcc tcccagatgg cagcatacag gatacgcggg
     2701 ttgtgcgggt ccatcgaaag gtcgatagcc cctgctttct cgctgcggaa caggatacgc
     2761 tcccaggtgc tgccgccgtc cttcgagcga tagaggccgc gttccgcgtt cgggccgtgc
     2821 gcgtgaccca gcgcggccac gtagaccagg tcgggattgc tcggatgaac gcgcactatt
     2881 gcgatattgc gcgtatcggc caggccgaga tgcgtccagg tctggccgcc atcggtggaa
     2941 cgatagaccc catcgccatg cgaaacgttg ctgcgaatcg tggtttcgcc cataccgaca
     3001 tagacaacgt tggggtctga tggcgcaacg gcaatagcgc caacggaggc gcgcttgaaa
     3061 taaccatcgg agacgttctc ccagatgata ccgccatcgg tagtcttcca gacaccgccg
     3121 cccgtcgagc caaaataaaa gacctgcgca tgcaccgggt cgcccgcgac ggctacaact
     3181 cgccctcctc ggaaaggtcc gatgcagcgc cattcgaggg tctgcaagag ggctggattg
     3241 acggcgggtt gcttggattg ttcgtctgcc gtaccgtttg aggaacgccg atgtacggtt
     3301 ccgttctcat ttgccataat acaatgtcca tttctacgcg caaataacgc ttcgcacccc
     3361 aggtatgtat ttccagggaa agtgtagcat atcgaaaatg gcttgtcgag caacccgaaa
     3421 tggtaacaaa aacggtttcg tttggctaca acttgttgct ctttggcatc agcgatatat
     3481 tgtaacctgc atgtgtta
//
LOCUS       DASZAK010001354         1907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_176869, whole genome shotgun sequence.
ACCESSION   DASZAK010001354 DASZAK010000000
VERSION     DASZAK010001354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176869"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VGT44_23700"
     CDS             complement(<1..386)
                     /locus_tag="VGT44_23700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEV2583879.1"
                     /translation="MHRAVRQRTILLVEADPALRRMITLGLQYRGLRVIDVDSPGELS
                     DMSKSNPDLVLLDIDGKVGNGVQSLAEVQANPTLTSLPIVMLTWDASTLISSGEELDD
                     ANETPARQSRYTTLAKPFDARSLYAM"
     gene            552..1673
                     /gene="queA"
                     /locus_tag="VGT44_23705"
     CDS             552..1673
                     /gene="queA"
                     /locus_tag="VGT44_23705"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV2583880.1"
                     /translation="MLEHSTYNISDFDYTLPQELIAQTPIEPRDSSRLLALHRATGAI
                     EHRHFREIGEYLRPGDLLIANQSRVIPARLMGRRAETGGAVEVLLLAERPDLGPDHWE
                     TLVRPGRRLREGAHIVFGDKMLSEMLHGEILQRTDAGGRVVRFWVAPNNVGAQFIAPR
                     PDNARTATVRHLIDKLGKMPLPPYIHETLQDPERYQTVYARISGSAAAPTAGLHFTPR
                     LLDELRKQGVRVGFVTLHVGLDTFRPVEVEDFREHKMHSEEIDLDEETAGLINETREA
                     GGRVVAVGTTAVRVLESVVSVYDGEVRPYHGATRLFITPGYRFRAVDAMITNFHLPRS
                     TLLLLVSSFATKALIEEAYREAIRERYRFFSFGDAMLML"
     gene            complement(1642..>1907)
                     /locus_tag="VGT44_23710"
     CDS             complement(1642..>1907)
                     /locus_tag="VGT44_23710"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEV2583881.1"
                     /translation="QAIQILNNIEAALIRAGASLKDVVRTRIYVINIDDWQQIGKAHG
                     EYFRDIRPATSMVEVRRLISPEILLEIEAEAIISTTSTSHRRS"
BASE COUNT          383 a          497 c          616 g          411 t
ORIGIN      
        1 atcatggcat acagcgagcg cgcatcgaat ggttttgcca gggtggtgta cctggattgc
       61 cttgccggtg tttcgtttgc atcatcgagt tcttcgcctg aactgataag agtggatgcg
      121 tcccaggtca gcatgacgat gggaagcgag gtcagcgtgg gattagcctg tacctctgcc
      181 agcgattgga cgccattgcc gactttgccg tcgatgtcga gcaagaccag gtctggattg
      241 ctcttgctca tatcggatag ttcgccggga ctatcaacgt cgatgacgcg caggccgcga
      301 tactgcaagc ccagggtaat cattcgccgc agggcgggat cggcctcgac gagcaggata
      361 gtacgttgcc gtacagcgcg atgcatggct acctctcttg atggtgtgat aagcgcgtca
      421 ttgtattgaa gatagccagt ggacattacg gcgaatttgc gaaaaaggga aaaatcctta
      481 cagccacctg aatagagtcg ttgcgaaaag cgagattgac aatcaagctc aatcacggta
      541 aagtgggcgc tatgttagag catagcacct acaacatcag cgacttcgac tatacgctcc
      601 cgcaagaact gatcgcgcag acgcctatcg aaccgcgcga ctcctcgcgg ctgcttgcgc
      661 tgcaccgcgc gacaggagct atcgaacaca ggcattttcg cgagattggg gagtatttgc
      721 ggccaggcga cctgctgatc gccaaccaga gccgcgttat ccccgcgcgc ctgatgggac
      781 gacgagccga gacgggcggc gcggttgagg ttctgctgct ggcagaacga cctgatcttg
      841 ggcctgacca ttgggagacg ctggtacgcc ctgggcgcag gttgcgcgag ggggcgcata
      901 tcgtctttgg ggataagatg cttagcgaga tgctgcatgg cgaaatattg cagcgcacgg
      961 acgcgggcgg gcgggtcgtg cgattctggg tagcgccaaa caatgtaggg gcgcaattca
     1021 ttgcgcccag gcccgataat gcgcgcaccg ccaccgtgcg gcaccttata gacaaattgg
     1081 gcaagatgcc gctgccgcct tatattcatg agacgttgca ggacccggag cgttatcaga
     1141 cggtatatgc gcgcatcagc gggtcggcgg cggctccgac ggcggggctg cacttcacgc
     1201 cgcgcctgct ggacgaactg cggaagcagg gtgtgcgcgt cggctttgtg acgctgcatg
     1261 tgggactgga tacgttccgg ccagtggagg tggaagattt tcgcgagcat aagatgcata
     1321 gcgaggagat cgacctggac gaagagacgg cagggctgat taacgagacg cgcgaggcgg
     1381 gcgggcgcgt ggtcgcagtc ggcacaacgg cggtgcgcgt actggagagc gtggtgagtg
     1441 tctatgatgg cgaggtgcgc ccctatcacg gcgcgacgcg gctctttatc acgcccggtt
     1501 atcgtttccg cgcggttgat gccatgataa ccaactttca cctgccgcgc tcaaccctgc
     1561 tgctgctggt cagctccttc gccaccaaag cgctgatcga ggaggcgtac cgggaggcga
     1621 ttcgggagcg ctatcgattc ttcagcttcg gcgatgcgat gttgatgttg tagagataat
     1681 ggcttccgct tcaatctcca agagtatctc cggtgaaatt aaccttcgta cctctaccat
     1741 gctggtggcg ggacggatgt cgcggaagta ttcgccgtgc gctttgccga tttgctgcca
     1801 gtcgtcgatg ttgatgacgt agatgcgcgt gcgtaccacg tctttgaggc ttgcgcctgc
     1861 tcttataagg gcggcttcga tgttgttgag gatttgtata gcctggg
//
LOCUS       DASZAK010001355         1906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_353884, whole genome shotgun sequence.
ACCESSION   DASZAK010001355 DASZAK010000000
VERSION     DASZAK010001355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353884"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..565)
                     /gene="acpS"
                     /locus_tag="VGT44_23715"
     CDS             complement(56..565)
                     /gene="acpS"
                     /locus_tag="VGT44_23715"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV2583882.1"
                     /translation="MMDDTNDAPMIFTPLVRQPGVAIPDWLFGPAGVAGLPAPLHTPQ
                     GVNIAIGIDIIEVDRVRKVHEHHGERFLRRVFTEAEIRQCRGKATRLAGRFAAKEAIS
                     KALGTGLHGVAWREMEVVQLRSGRPTVTLHGRAKQRAELLGLSAFDVSIADLQAFSIA
                     VAVAVQTAK"
     gene            complement(584..>1906)
                     /locus_tag="VGT44_23720"
     CDS             complement(584..>1906)
                     /locus_tag="VGT44_23720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEV2583883.1"
                     /translation="VTCVDIIPEKIARLKEGVLPIYEPGLEEMVERNVHAGRLHFTTS
                     YSEGLDNCDFIFIAVNTPSNDGQGGADMRYVESAARSIAQELDHYAIIINKSTVPVGS
                     GDVVSRIIRHSLARPDVGFAVVSNPEFLREGSAIADFQAPDRVVLGSTDEEAARHVAT
                     LYLPLRAPIVITDLYTAEMVKYASNAFLATKISFINEIAQICERLGADVKEVAAGMGY
                     DKRIGRAGLDAGLGWGGSCFPKDVRALAHMADGAGLHPQLLHAVMDINHDQRRLVVTK
                     LTSILGALRGCAIGVLGLAFKPNTDDMREAPSIDIIRWVTSQGAEVRVYDPVAAETGR
                     AALEREHVRMEQVAFCSDPYEVAQGIDALVIVTEWNEFKSLDMAQIRAAMRRPVLIDG
                     RNIYESAEMNRLGFIYRGMGRGTGPAPSVLPAGDSSASQPLLDGANGK"
BASE COUNT          332 a          567 c          634 g          373 t
ORIGIN      
        1 ttcgctgcgc tcaggatgac agtgccgatc ttgttcgtga aaattcatca tggcgttact
       61 ttgccgtctg cacggccacg gcgacggcaa tcgagaaggc ttgcaggtcg gcgatgctca
      121 catcgaaggc gctcaggccg agcagttcgg cgcgctgctt cgctctgccg tgcagggtaa
      181 cagtcggacg accgctgcgc agttggacga cctccatttc gcgccaggcc acgccgtgca
      241 ggccggttcc cagggctttg ctgatggcct ctttcgcggc gaagcgcccg gccaggcggg
      301 ttgccttgcc ccggcactgg cgaatctccg cctctgtgaa gacgcgccgc aggaaacgct
      361 cgccatgatg ctcgtggact ttgcgcacgc ggtcaacctc gatgatgtcg atgccgatgg
      421 cgatgttgac gccctgcggt gtgtgcagcg gcgcgggcag cccggccact cctgctggcc
      481 cgaagagcca gtcgggaatg gcgacccccg gctggcgcac caggggagtg aagatcatgg
      541 gagcgtcgtt tgtatcgtcc atcatcaggc gctttccagg tcattacttt ccattcgcgc
      601 catccaacaa tggttgtgac gcgctgctgt ccccggcggg caaaacggat ggggcggggc
      661 cggtgccgcg tcccatgccg cgatagatga agccgaggcg attcatctcc gccgattcgt
      721 agatgttgcg gccatcgatc agcaccgggc ggcgcatggc agcgcgaatc tgagccatgt
      781 cgagcgactt gaactcgttc cattcggtga cgatcaccag ggcgtcgatg ccttgcgcga
      841 cttcgtaggg gtctgagcag aaggcgacct gttccatgcg cacgtgttcg cgttccaggg
      901 cggcgcgccc ggtttcggcg gcgacggggt cgtagacgcg cacctccgcg ccctggctcg
      961 tgacccagcg aatgatgtca atcgagggcg cttcgcgcat gtcgtcggtg ttaggcttga
     1021 aggccagccc aagtacgccg atagcacacc cgcgcagcgc accgaggatg gaggtaagct
     1081 tggtgacgac gaggcggcgc tgatcgtggt tgatatccat gacggcgtgg agcaactgcg
     1141 ggtgcaggcc ggccccgtca gccatgtggg caagggcgcg cacatctttg ggaaagcatg
     1201 agccccccca tccgaggcca gcatccagtc cggcgcgtcc gatgcgcttg tcgtagccca
     1261 tgccggcggc gacctctttc acgtccgcgc cgaggcgttc gcagatttgc gcgatctcgt
     1321 tgatgaagga aatcttggtg gccagaaatg cattcgaggc gtacttaacc atttcagccg
     1381 tgtagaggtc ggtgatgacg atgggcgcgc gcaggggcag gtaaagcgtg gcaacatggc
     1441 gcgccgcctc ttcgtctgta gagccaagca ccacacggtc gggtgcctgg aaatcggcga
     1501 tggcggagcc ttcgcgcagg aattcggggt tggagacgac agcaaagcct acatcgggcc
     1561 gggccaggct gtggcgaatg atgcgcgaga ccacgtcgcc gctgccgacc ggcacggtgc
     1621 ttttgttgat gatgatggcg tagtggtcaa gctcctgggc gatgctgcgc gccgcgctct
     1681 cgacgtagcg catgtccgcg ccgccctgcc catcgttgct tggtgtattg acggcgataa
     1741 aaatgaaatc gcagttgtcc agcccctcgc tatagctggt ggtgaaatgc aggcgaccag
     1801 cgtggacgtt gcgttcgacc atctcttcca gacccggttc ataaatcggt agaacgcctt
     1861 ctttgaggcg tgcgatcttc tcagggatga tatcgacgca ggtgac
//
LOCUS       DASZAK010001356         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_566933, whole genome shotgun sequence.
ACCESSION   DASZAK010001356 DASZAK010000000
VERSION     DASZAK010001356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566933"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2905
                     /locus_tag="VGT44_23725"
     CDS             <1..2905
                     /locus_tag="VGT44_23725"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2583884.1"
                     /translation="RRVVISGSGTLALESAEILRHRGYEVTHLLRGQTLWSEVLDPVA
                     SDLVLEEERRDGIDVRTGEEIAEIVGRNGQAAQVVTTRGERIACELVLIAIGIEPLIE
                     FIRASGIACGRGVQVDSGMRTSVPDIFAAGDVIETRDPHSGRTRVLGQWFPAIQQAQV
                     AAYNMLDLLPPGHPLAPAAQRDATTAYMNYYNATLLYGLDFVSVGLTTRPHAPGYTEI
                     VADPQPRGYRKAILKQGVMVGALLLGNRRGGLAFKRAIDARVNVMPVARQLFDDAFDL
                     DAWLDSQHVPEANINLSLGDDPSQAERSRTMVAGLSGQTPTVGVAHRQALATLDEQRV
                     RFEIDMCIGCNRCMDACPVPMSSLVTIADLNSANVTATIAPQVARFTHECIMCGSCVP
                     VCPVDNHRDLLMLSLKERLGVSWKHMPDTKHIKYMAEQLPAGWTVPMLLARLREQAIL
                     RDVGQVPDTYLLHMLAVSNALVIMPGETLFREGEYGRDLRLILEGRVELMATDIEGNE
                     LPLAILRRGEYAGEDGMLTGLPYKATATAQAPTFVLEVPEQVMQRLMELVPEVRSHFE
                     RYDSARSLQAILKRLALFQGVADSDIQALIRRTPVKQYDRDERLFAEDDRGGRPARET
                     LHILLEGFVKVARHAAKGTGQRRGDERILAYRQGGDYFAGGLDLLGDGQAVTVTTINR
                     CRVAEVPRSALLALFQQYPEVGQRFTARLREYVETAVSSQGYALASGPLARFAPDSRP
                     VDAHAQDGLHALVSDGVVEGTGVLVIDLDKCIHCDECEEACRRRHGHSRMNRKGMVVG
                     NISIATACRQCQDPVCMLCSRAGIARHPNGEVYITDSCIGCGICAERCPYGAISIVNI
                     DDEPAALFSLDRFRQFFAKGAHNASERKPTRTLPMVSADVVGSYTAPGPLDVHAPRHA
                     FDELRKKVAIKCDLCAGYSDQACVQACPTGAAVRISPVQFFGSTEEILRRRVR"
BASE COUNT          544 a          914 c         1019 g          536 t
ORIGIN      
        1 gcggcgtgtg gtaatcagcg gcagcgggac tctggcgctg gagagcgcgg agatcttgcg
       61 gcatcgcggc tacgaggtga cgcacctgct gcgcggacag acgctctggt cagaggtgct
      121 tgacccggtg gcgtccgacc tggtgctgga agaggagcgg cgcgatggca tcgacgtacg
      181 caccggggaa gagatcgccg agatcgtcgg gagaaatggc caggctgcgc aggtcgtcac
      241 aacacgcggg gagcgcatcg cctgcgagct ggtcttgatc gccatcggca tcgagccgct
      301 gatcgaattc attcgcgcga gcggtattgc ctgcgggcgc ggtgtgcagg ttgatagcgg
      361 catgcgcacc agcgttcccg atattttcgc cgccggggat gtgatcgaga ctcgcgatcc
      421 gcactccggg cgcacgcgcg tgctggggca gtggtttcct gcaatccagc aggcgcaggt
      481 agcggcctac aatatgctcg acctgctccc gccgggacat ccattggccc ctgctgcaca
      541 gcgcgatgcg acgacggcgt atatgaacta ctacaacgcc acgctgctct acggcctgga
      601 ttttgtgtcg gtagggctaa cgacacggcc acacgcgccg ggctatacag aaatcgtggc
      661 cgacccccaa ccgcgcggct accgcaaagc catcctcaag cagggcgtca tggttggcgc
      721 gctgctgctg ggcaaccgcc ggggcgggct ggccttcaaa cgcgccatcg acgcgcgcgt
      781 gaacgtgatg ccggtcgcgc ggcaactctt cgatgatgcc ttcgaccttg atgcgtggct
      841 cgactcgcag cacgtgccag aggcgaatat caatctctcg ttgggcgatg acccatccca
      901 ggctgagcgc agcagaacga tggtggcagg tttgagtgga caaacgccca ccgttggcgt
      961 ggcgcatcgc caggcgctgg caacgctgga cgaacagcgc gtgcgcttcg agattgatat
     1021 gtgcatcggc tgcaatcgct gtatggacgc ctgcccggtg cccatgtcct cgctcgtcac
     1081 gattgccgac ctgaatagcg ctaatgtcac cgcgacgatc gcgccccagg tggcgcgttt
     1141 tacgcacgag tgcatcatgt gtggctcgtg cgtgccggtc tgcccggtgg ataatcaccg
     1201 cgacctgctg atgctctcgc tcaaagagcg gctgggcgtt tcctggaagc atatgccgga
     1261 cacgaagcac atcaaataca tggcggagca gctccctgcc ggttggacgg ttcccatgct
     1321 gctcgcccgg ctacgcgaac aggccatttt gcgcgacgta gggcaggtgc cggataccta
     1381 cctgctgcac atgctggcgg tctcgaatgc gctcgtcatc atgccagggg agacgctctt
     1441 tcgcgaagga gaatatgggc gagacctgcg cctgatcctg gagggtcgcg tggagctgat
     1501 ggcgaccgat atcgagggca acgagctgcc cctggcgatc ctgcgccggg gcgagtacgc
     1561 gggcgaagat ggtatgctga ccggcctgcc ctacaaggcg acggccacgg cgcaggcccc
     1621 cacattcgtg ctggaagtgc cggagcaggt gatgcagcgc ctgatggaac ttgtgccaga
     1681 ggtgcgcagc cactttgagc gctatgactc ggcgcgctcg ttgcaggcca ttctcaaacg
     1741 cctggcgttg ttccagggcg tggcggattc cgacatccag gcgctgatcc gcagaacacc
     1801 ggtcaagcaa tacgaccgcg acgaacgctt attcgccgag gatgatcgag gtgggcgacc
     1861 ggcgcgcgag acgctgcata ttttgctgga aggctttgtc aaagttgccc ggcacgcggc
     1921 gaaggggacc gggcagcgca ggggcgacga gcgcattctg gcctatcgcc agggtggtga
     1981 ctacttcgcg ggcgggcttg atttgctggg tgacggccag gcagtcacgg taacgaccat
     2041 caatcgctgc cgcgtcgccg aggtgccgcg ctccgcgctg ctggcgctct tccagcagta
     2101 cccggaggtg ggccagcgtt tcacggcgcg cctgcgcgaa tacgtcgaaa cggcggtttc
     2161 atcacagggg tatgccctgg cgagcgggcc gctggcgcgc ttcgcccccg acagcagacc
     2221 ggtcgatgcc cacgcgcagg atggcctgca cgcgctggtg agcgatggtg tggtggaggg
     2281 taccggcgtg ctggtgattg acctggataa gtgcattcac tgcgatgagt gcgaggaggc
     2341 gtgtcggcgg cgacacggcc attcacgcat gaaccgcaaa ggcatggtgg tcggcaatat
     2401 cagcatcgcc accgcctgcc gccagtgcca ggaccccgtc tgtatgctgt gcagccgtgc
     2461 cgggatcgcg cgccatccca acggcgaggt ctacatcacc gatagctgca tcggctgcgg
     2521 catctgcgcg gaacgctgcc cctacggcgc gatctcgatt gtgaacatcg acgacgaacc
     2581 ggcggcgctc ttctcgcttg atcgattccg ccagtttttc gcgaaagggg cgcataatgc
     2641 gagcgagcgt aagccaacca ggacgctgcc aatggtcagc gcagatgtgg tggggagcta
     2701 taccgcgccg ggtccgctcg atgtccacgc gccccgtcac gccttcgacg aactgcgcaa
     2761 aaaagtggcg atcaagtgtg acctctgcgc gggctatagc gaccaggcgt gcgtgcaggc
     2821 ttgtccgacc ggcgcggcgg tgcgcatctc gcccgtgcag ttctttggct caaccgagga
     2881 gattttacga cgacgtgtac gataaagcgt taatggttcg gaagggcgac cacaagggtg
     2941 cgcccctgcc ggtggttccg agggcgaccg caagggtacg cccctacaat gaccacgaag
     3001 ggactgcgca cat
//
LOCUS       DASZAK010001357         2400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_139845, whole genome shotgun sequence.
ACCESSION   DASZAK010001357 DASZAK010000000
VERSION     DASZAK010001357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139845"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..1180)
                     /locus_tag="VGT44_23730"
     CDS             complement(170..1180)
                     /locus_tag="VGT44_23730"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEV2583885.1"
                     /translation="MQCSSCGTQLPDEAAYCPTCGSVTPSQVSSSGVSPSDPTSVYTS
                     YGDSSKNPYAADDPYAAPLTAPPPPPVRRRSPPAAMIALLIALVLLVIAAGGIFFLRT
                     AFSPGQVQTHASPTATVPQATTTPSQEPQATATLSAQSPQSLYDQITQGAPVLDDPLL
                     RNDTNNWAEGASSDGQMSCVFTGGAYHAIAQPKNSYMLCTAQATNFGDLAYQVQMSIV
                     KGEFGGMVFRADGSQSKYYSFFIDRSGNYTLITSVDNTGNNDYVLRQGTSAFIRTGLN
                     QLNTLAVIARGGNIYLYINQQYLTSASDSRYHIGQIGVFGGNVTQAPADVVFSRVQVW
                     NL"
     gene            complement(1348..1779)
                     /locus_tag="VGT44_23735"
     CDS             complement(1348..1779)
                     /locus_tag="VGT44_23735"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV2583886.1"
                     /translation="MNTSDFDEALEHYHRAASEFVKGNPEPYKMVFSHREDVSLANPF
                     GPVVRGWKQVAETMERAASLYREGEVIGFENVAKYVTADLAYIVEVERIKARIGGREE
                     VASLALRTTSILRPEDGTWRVLHRHADPITTVRAPESVIQQ"
BASE COUNT          538 a          687 c          645 g          530 t
ORIGIN      
        1 acttgaccgg cgcgtcaact tcctctcacg ctcgcgcaag cgcgcaacgc acttcgtccc
       61 tgattgggac atcgacctgc atgtcaatga gcattccttt ggcctcaaag agacgggctt
      121 cggtcaacca accgccgagc cgagaccagg ctatccggtc cccggttttt cacaggttcc
      181 acacctggac acgactaaac accacatctg ctggagcctg ggtcacgttg ccgccaaaca
      241 ccccgatttg accgatgtgg tagcggctgt cactggcaga ggtgaggtat tgctgattga
      301 tgtacaggta gatgttgccg ccgcgcgcaa tgaccgcaag ggtgttgagc tggttaaggc
      361 cagtcctgat gaacgcggaa gtgccctggc gcaacacgta atcgttgttt cccgtgttgt
      421 cgaccgaggt gatcagcgtg tagttcccgg agcgatcgat gaagaaggaa taatacttcg
      481 actgcgaccc atcagcacgg aacaccatcc caccaaactc ccctttgacg atactcatct
      541 ggacctggta ggccaggtcg ccaaaattgg tagcttgagc cgtgcacaac atgtacgaat
      601 tcttcggctg ggctatagca tgataagcac cccctgtgaa tacacagctc atctgcccgt
      661 ccgaagaagc accctcggcc caattgttgg tgtcattcct cagcaaagga tcatcaagca
      721 ctggcgcgcc ttgcgtaatc tgatcatata ggctctgcgg cgactgagcg gagagggtgg
      781 ccgtggcttg aggctcctgg gaaggagtgg tcgtcgcctg cggcactgtc gcggtagggg
      841 aagcgtgagt ctgtacctgg ccaggggaaa aagccgttct caagaagaat ataccacccg
      901 ccgcgataac gagtagcacg agggcgatca gcagagcaat catcgccgct ggtggactac
      961 ggcggcggac aggaggcggg ggaggcgctg tcaaaggagc agcgtaagga tcatcggctg
     1021 cataaggatt cttcgaagag tcaccgtagg acgtgtagac agacgtcggg tcagacgggg
     1081 acaccccgga ggaagaaacc tggctgggcg taacgcttcc acaggtagga caataggccg
     1141 cttcgtcggg cagctgcgtt ccacagctac tacattgcat caaagcctcc ttctcatgca
     1201 tttagcgacg ctggttttca atgagtactc ggataaacag tggaattttc atgccttaat
     1261 ggtaccacat tctcgcttac cttccttgag aaggactcaa gacaagcggc catacatgcg
     1321 ctccgacctg gatggtttgc gactgagcta ctgctgtatg accgattccg gtgcccgaac
     1381 ggtggtgatg ggatcggcgt gtcgatgtag caccctccag gtgccatctt ccggtcgaag
     1441 aatgctcgtc gtccgcagag ccagtgaggc gacctcctcc cttccgccga tcctggcctt
     1501 gatccgctcg acttccacaa tgtaggcaag gtcagcagtc acgtacttcg caacgttctc
     1561 gaagccaatg acctcgccct ctcggtacag cgatgcggcc cgctccatgg tctcagcgac
     1621 ctgcttccac ccgcgcacga cagggccaaa ggggttagcg aggctaacat cttcccgatg
     1681 ggagaatacc atcttgtaag gctcagggtt gcccttcaca aactcgctgg ccgctcgatg
     1741 atagtgttca agtgcttcgt caaaatcgct tgtattcata agccatcatc tcctccgtgc
     1801 tcaagtatcc taaagtgaac agatacatca cagaggctgg agaacaacaa aattcttgta
     1861 ctcggatcaa gaggcacacc ccgcgtaagg agagaaccga tgttcctgtc aaccccgttt
     1921 ttccccgcaa acaaaaacgc ctcaaacggc ctgtagagcc aattgagacg cctctgaagt
     1981 tcttctgagt gccgaagggg ggactcttgc tcatctgcct atcatgaatg cctctgcatt
     2041 ggcttctcac cgtccttgag agtctattac tcgccgcgga gagcggacaa gtgcagttct
     2101 tctgtcagtt tatcccccta aaaaccgcca aatcggatca tactcagtct ccttcctccg
     2161 ctctctctac gattctgttt ccattctcga ttggctgtgc tatactgacg ttgtcttcct
     2221 caatccttaa tcttgtccgt atgtgttcag ataagggcag aaaggtcatc acatgtcgct
     2281 ggaaggacgg cacctcggtc actaccgcct gctgcgcctg cttggcagcg gaggcatggg
     2341 cgaggtctac ctggctgaag atgcccgcat cggccagcag gtcgccatta aggtcatcag
//
LOCUS       DASZAK010001358         5785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_176986, whole genome shotgun sequence.
ACCESSION   DASZAK010001358 DASZAK010000000
VERSION     DASZAK010001358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5785
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176986"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1320
                     /locus_tag="VGT44_23740"
     CDS             127..1320
                     /locus_tag="VGT44_23740"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEV2583887.1"
                     /translation="MMSVTSRRELLAVVAPRYRAARGEERSRILEEFVASTGYHRKYA
                     LSVLNHPITKGTARKKRVRPRQYVFAVQQALVTCWRATNGICSKRLAPYLPELVRVLE
                     QHGELHLEAQTKARLLALSPATADRLLQAERTRAKPHGLGTTKPGTLLKSAIPIRTFA
                     EWDEAEPGFTEIDLVVHCGTTTRGEYLHSLTVTDVATGWTECVALRNRGQQSVFQALV
                     LARGHLPFPLRGIDSDNGSEFINAHLLRYCQDEQLTFTRSRPYKKNDQAYVEQKNWSI
                     VRQLVGYERYESLSAYEALCALYEVVRLYVNFFQPSMKLLTKERIGSKIKKTYDPAKT
                     PYQRVLESEQVSEEIKACLRQQYATLNPVALLRQMQRLQAVLWKLAVGEPVELETAQT
                     AAATS"
     gene            1680..2642
                     /locus_tag="VGT44_23745"
     CDS             1680..2642
                     /locus_tag="VGT44_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEV2583888.1"
                     /translation="MSASTSARLQSEDALLERIRRQQLVESVDHMSPIYLEGMKRILT
                     VSADTELISAPAYYHAAVHAPSLNTFGSAMSIIQDELSHAHIAYRLLRDLGVDTEQLI
                     FEREPHNFKYPYAFDVPLDSWVELLVANAFYDRAGYTLLGDVHQSTTFGPWKRALVKV
                     DKEETFHLRHGEQGMRKLNQDASGHEALQQAVDWMFMLTVEWFGLPDSLKKHGEQLEY
                     GFKGHSNDELRQIWMSTAVPLCEELHLNVPAHYDKEQQKYIVDCPFPAHFDAKAKRWL
                     FEDGPCTWDDVVKRWKARGPANEEFVGMLQRGKREMLRLLEQDA"
     gene            2639..2992
                     /locus_tag="VGT44_23750"
     CDS             2639..2992
                     /locus_tag="VGT44_23750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV2583889.1"
                     /translation="MSTTYPSLRTHMPCNSDCPALWDALRDVTDPEIPISVVDMGLIV
                     DLSLRDGVAHVKLTFTAMGCPATEFIMDDIRARLLQEPGVREVRIETVWDPAWTKARL
                     SEEGIDVMRYWGISA"
     gene            2989..3231
                     /locus_tag="VGT44_23755"
     CDS             2989..3231
                     /locus_tag="VGT44_23755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583890.1"
                     /translation="MSNTTETSIEHKSRREALNENRVWHVFARRKYEEPLYEIGNVMA
                     DDPELARVYARSIYDEFSWVEMALVPRDSIVIVIES"
     gene            3241..3993
                     /locus_tag="VGT44_23760"
     CDS             3241..3993
                     /locus_tag="VGT44_23760"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEV2583891.1"
                     /translation="MPTTLVDNAALFAVLSSLADNKQAVGRRYAYWSNGAPALEAAVA
                     AAAMTQDELGHARTLYPLLEDFAQAQANLVEVEPMTRTLRYHLTYLDHDFRGWSDFVA
                     TNFLFDTALTTFFEAAQNSSYEPLRARSRKIIQEERIHAMHAEGWVNRLAKTGGAVRA
                     ALVFALQRLWNETLCWFGPHDDPAMQQLYQQGIIDATPDELRSRYLPKIMPTLTSLDI
                     SVAATFNAASKRWEPSQPLPWRQWNAVSRRLE"
     gene            4346..5146
                     /locus_tag="VGT44_23765"
     CDS             4346..5146
                     /locus_tag="VGT44_23765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007908087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEV2583892.1"
                     /translation="MTTTSEKTLVNYTAVEGVGVIELTNPPANTYSYEMMRQLDEAIL
                     SARFDENVHVIVIRGAGEKFFCAGAEIAMLNAVTPTFKYYFCLHANETLTRLEQTPKL
                     VIAALNGHTVGGGLEIALACDIRIARRDAGKVGLPEINLGVLPGTGGTQRMARALPKN
                     KAIELMTEGKLISFEQALDLGLINYIYDSENYWNDVMAYARQFCPPNKASKTVGRIKR
                     AVQSGAEVAFSESLAIERELQQLCFQSEDAKEGLAAYAEKRKGQFAGR"
     gene            5146..5739
                     /locus_tag="VGT44_23770"
     CDS             5146..5739
                     /locus_tag="VGT44_23770"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEV2583893.1"
                     /translation="MSHIRIRHQNDILWLILDRPPLNILSIEMLDQLTAAMRQAIERE
                     PRLVVLMGAGERAFSAGVDVRDHLDGREREMLRAVYDNCAAFDALRTARIPSVALVKG
                     HALGGGCELAVLCDTVIAHEDATFGLPEINLSVFPPVAAAYFPARIGSQATMRLILTG
                     ETIPAREAFRLGLAHQVLPDRRFLPDAEELLAMLASA"
BASE COUNT         1255 a         1819 c         1667 g         1044 t
ORIGIN      
        1 tgaggagaaa gacaatactc cgaattgact caccaaaaat cttactttgg ccgaaacgtt
       61 gcctcatgag agaatcttac cagccgctgt tgtggaagag ccaggcggct ggaaggaaga
      121 gaggccatga tgagtgtcac gagtcggcga gagttgttag cggtggtggc cccgcgctac
      181 cgagcagccc gaggagagga acgcagtcgc atcctggagg agtttgtggc cagtacgggc
      241 taccatcgca agtatgcctt gagcgtgctc aatcacccga tcacgaaggg aacggcccgc
      301 aagaaacggg tgcgtccgcg tcagtatgtc tttgcagtgc aacaagcgct ggtgacgtgc
      361 tggcgcgcca ccaatggcat ttgcagcaag cgcctggcgc cgtacctgcc cgaactcgtg
      421 cgggtgctgg aacagcacgg ggaactgcac ctggaggcgc agacgaaagc gcgcttgctg
      481 gcgctgagtc cagcgacggc tgatcggctc ttgcaggccg agcgcacgcg tgccaaaccg
      541 catgggttgg gcaccaccaa gccggggacg ctgctcaaga gtgccatccc cattcgcacc
      601 tttgccgagt gggatgaggc cgagccaggt tttacggaaa tcgatctggt ggtgcattgt
      661 ggcacgacca cacgtgggga gtatctgcat agcctgaccg tgaccgacgt agccaccggc
      721 tggaccgagt gtgtggcgct gcgcaatcgc ggccaacaga gcgtcttcca agcgttggtg
      781 ctggcgcggg gacacttgcc gtttcccctg cgcggcatcg actcggataa cggcagcgag
      841 ttcatcaacg cgcatctact tcgctattgt caggacgaac agctcacctt cacccgctcc
      901 cgcccgtaca agaaaaatga ccaggcgtat gtggagcaga agaactggtc gattgtgcgc
      961 caactggtag gctacgaacg ctatgaaagc ctgtcagctt atgaagcgct gtgcgccctc
     1021 tacgaggtgg tgcggctcta cgtcaatttc ttccagccga gcatgaagtt gctcaccaaa
     1081 gagcggatag gcagcaagat caaaaaaacc tatgatcccg ccaagacgcc ttaccagcgc
     1141 gtgctggagt ccgagcaggt cagcgaggag atcaaagcgt gcctgcgcca gcagtacgcc
     1201 acgctcaatc cggtggccct gttgcgccag atgcagcgac tgcaggccgt gctctggaag
     1261 ctggccgtcg gggaaccggt tgagttagag accgctcaga cggccgcggc tacttcttga
     1321 caagtgggtg tcgctgtggc atgaatgaag cagccccgcc cggcgtgtcg ggcggcaact
     1381 ttggcgggag cggcggcgcg ccgccgctcc cgccaaagtt ggattgttag ttgaggcaac
     1441 gaatgcccga aagtaagatt tttaggtgag gcaacacgta tgcagcgaat atgcaacccg
     1501 taggagccgg tttaccgtgc tcctacgggg cttccaaccc cctcttgact ttcccaatat
     1561 tattgtttac aattctacca gtcgttcaaa tttgtgatgg aactgtgatt tcgccctcac
     1621 cgttcgcaag gacatatctg taaaccggac atgccctgcc agggcaagga gtaccgccta
     1681 tgtctgcatc tacaagcgcc aggctgcaaa gcgaggatgc gctgcttgag cgtatccgca
     1741 ggcaacaact ggtcgagtct gtcgatcata tgagtcccat ttatctcgag ggcatgaagc
     1801 gcatcctgac cgtttccgcc gataccgaac tcatcagcgc gcccgcctac taccatgctg
     1861 ccgtccacgc gccctcgctc aatacctttg gttcggccat gtcgatcatc caggatgaac
     1921 tgagtcacgc gcacatcgcc tatcgcctgc tgcgcgacct gggcgtcgat accgagcagt
     1981 tgatcttcga gcgtgagccg cacaacttca aatatcccta cgcctttgac gttcccctgg
     2041 atagttgggt ggaactgctc gtcgccaacg ccttctacga ccgcgcgggc tataccctgc
     2101 tgggcgatgt ccaccagagt accaccttcg gcccctggaa acgcgccctc gtcaaagtgg
     2161 acaaagaaga gaccttccac ttgcggcatg gtgagcaggg gatgcgcaag ctcaatcagg
     2221 acgcgagcgg gcatgaagcg ctgcaacagg cagtagactg gatgttcatg ctgaccgtcg
     2281 aatggttcgg cctgccggac agcctgaaaa agcatggcga gcagcttgaa tacggcttca
     2341 aggggcatag caacgacgag ttgcgccaga tatggatgtc tacggcagtt ccgttatgcg
     2401 aggaactgca cctcaacgtt ccggcgcact acgacaagga gcagcaaaag tacattgtcg
     2461 attgcccctt cccggcgcat ttcgacgcga aggccaaacg ctggctcttc gaggatggcc
     2521 cttgcacctg ggatgatgtc gtgaaacgct ggaaggcgcg cggccccgcc aacgaagagt
     2581 tcgtcggcat gctccagcgc ggcaaacgcg agatgctgcg ccttttggag caggacgcat
     2641 gagtaccact tatccatcgc tgcgcacaca tatgccctgt aatagcgact gcccggccct
     2701 atgggacgcc ctgcgcgatg tcacggaccc ggaaatcccc atcagcgtcg tggatatggg
     2761 cctgatcgtg gacctctccc tgcgcgatgg cgtagcgcac gtcaagctga ccttcacagc
     2821 catgggctgc cccgccacag aattcattat ggacgacatc cgcgcgcgcc tgctccaaga
     2881 accgggtgtg cgcgaagtgc gcatcgaaac cgtctgggac cccgcctgga caaaggcgcg
     2941 cctgagcgaa gagggcatcg acgtgatgcg ctactggggg atttcagcat gagcaatacg
     3001 acagaaacta gcatcgaaca caagagccgc cgtgaggcgc tcaacgagaa ccgggtctgg
     3061 cacgtcttcg cgcggcgcaa gtacgaagag ccgctgtatg agatcggcaa cgtcatggcc
     3121 gacgacccgg aactggccag ggtctacgcg cgcagcatct acgacgaatt ttcctgggtt
     3181 gagatggcac tggtgccgcg cgatagtatt gtgatcgtca tcgagtcata ggaagaaacc
     3241 atgcccacaa cgcttgtcga taacgccgcg ctcttcgccg tcctgtcatc gctggccgac
     3301 aacaagcagg ccgtcgggcg gcgctacgcc tactggtcga acggcgcgcc cgccctggaa
     3361 gcggcagtcg cagcagccgc catgacgcag gacgagctgg gccacgcgcg cacgctctat
     3421 cccctgctgg aagatttcgc gcaggcccag gccaatctcg tcgaggtcga gccgatgacg
     3481 cgcaccctgc gctatcacct cacctacctc gaccacgact tccgcggctg gtcggacttc
     3541 gtcgccacca acttcctgtt cgataccgcc ctcaccacct tcttcgaggc tgcgcagaac
     3601 tccagctacg aacccctgcg cgcgcgttct cgcaagatca tccaggagga gcgcatccac
     3661 gccatgcacg ccgaaggctg ggtcaaccgg ctggcgaaaa ccggcggcgc ggtgcgagcc
     3721 gccctcgtct ttgccctgca gcgcctgtgg aacgagacgc tctgctggtt cggcccgcac
     3781 gacgatccgg ccatgcagca actctaccag cagggcatca tcgacgccac gccggacgag
     3841 ctgcgcagcc gctacctacc aaaaatcatg cccacactca ccagcctgga catcagcgta
     3901 gccgctactt tcaacgccgc cagcaaacgc tgggaaccat cccaaccact cccctggcgg
     3961 caatggaacg ccgtctctcg tagattggaa taaaatcgtt ggaaccggtt atgcaaatat
     4021 cccgtaggtg ccgatttatc ggccactatg ggcgttaacg gcaggcattc aggccgataa
     4081 atcggcgatg tgcgcgataa atcggcacct acgatcatac gggattagct atgcgatata
     4141 atgcagaaga accaaaaaag gaaacgcagg cgcgggcaaa accaccctgc cccttctgcg
     4201 gctcaaccaa caccgaacca ttcgcattgt tcggccagca attgctgacc gtacaaatgt
     4261 actgtaatac ctgtcacacc ccttttgaat gcgtgaaaga cgacaacatt ctagaggatt
     4321 ttgcagcacg aaaggatacc caggcatgac caccacgagc gaaaaaacac tggtgaacta
     4381 caccgccgtc gagggcgtcg gcgtcattga actgaccaac ccgcccgcca atacctacag
     4441 ctacgagatg atgcgccagc tcgacgaggc tattttgtcg gcgcgcttcg acgaaaatgt
     4501 gcatgtcatc gtcattcgcg gcgcgggcga aaagttcttc tgcgcgggcg cggaaattgc
     4561 catgctcaat gccgtgacgc ccaccttcaa atactacttc tgcctgcacg ccaacgaaac
     4621 cctgacccgc ctggagcaga cgcccaagct cgtcatcgcc gccctcaacg gccacactgt
     4681 cggcggcggc ctggagatcg cgctcgcctg tgatatccgc atcgccaggc gcgacgcggg
     4741 caaagtcggc ttgccggaga taaacctcgg cgtgcttccc ggcaccggcg gcacccagcg
     4801 catggcgcgc gccctgccca aaaacaaagc catcgaactg atgaccgagg gcaagctgat
     4861 tagcttcgag caggccctgg acctcggcct catcaactac atctacgact ccgaaaacta
     4921 ctggaacgac gtaatggcct acgccaggca gttctgcccg cccaacaaag cctccaaaac
     4981 ggtaggccgc atcaaacgcg ccgtgcaatc tggcgcggaa gtcgccttct ccgagtctct
     5041 cgccatcgaa cgcgaactcc agcagctctg cttccagagc gaggacgcca aggaaggtct
     5101 cgccgcctac gccgagaagc gcaaaggcca gtttgcagga agataatgtc tcacattcga
     5161 attcgccacc agaacgacat cttgtggctc atccttgatc gaccgccgct caacattctc
     5221 tcaatagaga tgctcgatca gttgacggcg gccatgcgcc aggctatcga gcgagagccg
     5281 cgcctggttg tgctgatggg cgctggcgag cgtgctttca gcgcgggcgt ggatgtgcgc
     5341 gatcacctgg atgggcgcga gcgcgagatg ctgcgcgcgg tctacgacaa ttgcgccgcc
     5401 ttcgatgcgc tgcgcaccgc ccgcatcccc agcgtcgcgc tcgtcaaggg acacgccctc
     5461 ggcggcggct gcgaactggc cgtgctgtgc gataccgtca tcgcgcacga agatgccacc
     5521 tttggcctgc cggaaatcaa tctgagcgtc ttcccacccg tcgccgccgc ctactttccc
     5581 gcgcgcatcg gcagccaggc caccatgcgc ctgatcctca ccggcgaaac catccccgcg
     5641 cgcgaagcct tccgcctcgg ccttgcccac caggtgcttc ctgaccgccg cttcctaccc
     5701 gacgccgaag aactgctggc catgctggcc agcgcgtagg gacaggggga agcggaatgg
     5761 agcggcgggg gccttgtgcc caata
//
LOCUS       DASZAK010001359         2963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_318232, whole genome shotgun sequence.
ACCESSION   DASZAK010001359 DASZAK010000000
VERSION     DASZAK010001359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318232"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /locus_tag="VGT44_23775"
     CDS             complement(<1..2231)
                     /locus_tag="VGT44_23775"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2583894.1"
                     /translation="MHDQARVVIIGSGIAGSSIAYHLTERGWRDIVVLEQGTLSSGTT
                     SHAPGLVGQLRSSISLTKMLVSSISLYKQLKVDGNPGYFEVGSLRLASSKERMEELKR
                     QVGFSKSAGLEAELISASEAKDMFPMMSLEGVEGALFLPTDGSAKAHVLAQALATIAQ
                     QRGATFYANTRVTGIEVVNGRVQAINTSRGRIRTEIVIAAAGIWSPRIGRLAGISIPL
                     IPMQHQYVVTGPMAELAGETSIPNLRDPDNLVYFRQDGNSMVLGGYEHDPAAFDVDAI
                     PDNSHPTVQSFNPPRFESLMQGCIARVPSLRDVELVKRVNGLESFTPDGEFILGESPD
                     VKGFWAACGFCAHGVSGAGGVGKMIAEWIVDGEPSLDLWEMDIRRFGAYTASRRYIAT
                     RVNEIYATYYDISYPMRERSSARKLRLSPVYYRLEELGAVFGEKAGWERPNWFASNEA
                     LAEDQGWPVPYSWASRYWSPAIGAEHQATRERVAMFDETSFSKIEVLGPGSLAFLQRI
                     TDNQMDQPVGTITYTQMPNEHGGIECDLTVTRLAADRFQIITGTAFGNHDLSWIRSQM
                     PTDGSVYVNDITSSRCCIGVWGPRTRHLVQQVSEDDFSNEAFPYLTAQNVTIGNIPVL
                     ALRVTYVGELGWEIYAPMEYGLKLWDTLWEAGQPLGMLAAGYRAIESLRLEKGYRYWS
                     ADIHSEYNPYEAGLGFAVKLQKGDFLGREALERIKARGVTRKLCCLILDDPAAVALGG
                     EPLL"
     gene            complement(2277..>2963)
                     /locus_tag="VGT44_23780"
     CDS             complement(2277..>2963)
                     /locus_tag="VGT44_23780"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEV2583895.1"
                     /translation="VGAEVLHFLAGEDVLVTRFIVGTGISPELAAQDETLRRIVHSMK
                     RYHAGPDFPGAFSPFETVRNYHTLALEYGVSFPDTLPQVFELMARIEQALAQSGTAYT
                     KPCHNDLLASNFIDDGHTIRILDWEYAGMGDIFFDLGNFAVNQSLNEEQCELLLHYYF
                     GEVRSAELAHLHLMRLGSDLRESFWGFLQMGISELDFDYREYAHHHLNRFLRNVETSP
                     FEQWLKDVKV"
BASE COUNT          658 a          874 c          777 g          654 t
ORIGIN      
        1 agcagtggct ctcctcccaa tgcaaccgct gctggatcat ccaggatcaa acaacataat
       61 ttgcgagtta caccccgcgc tttaatgcgc tccaatgctt cccgccccag gaaatcgcct
      121 ttttgtaact tgacagcaaa acccagcccc gcttcgtacg ggttgtactc cgaatggata
      181 tcggcgctcc agtaacgata tcccttctcc aggcgcagcg actcgattgc cctataccct
      241 gccgccagca tacccagcgg ttgcccggct tcccacagcg tatcccataa cttgagtcca
      301 tactccattg gcgcgtaaat ctcccagccg agttcgccca cgtacgttac acgcagcgcg
      361 agtaccggga tgttgcctat cgtcacgttt tgcgctgtca ggtaaggaaa agcttcattt
      421 gagaaatcat cctcacttac ctgctgcacc aggtggcgcg ttcgcggtcc ccatacgcca
      481 atgcagcagc ggctcgacgt aatgtcgttc acatagaccg agccgtccgt gggcatctgg
      541 ctgcgtatcc acgacaggtc atggttacca aaagccgtgc cggtgataat ctggaagcgg
      601 tcggccgcca ggcgtgtaac tgtcaggtcg cactcgatgc cgccatgctc gtttggcatc
      661 tgtgtatagg tgatagtccc taccggctgg tccatctggt tatcggtaat ccgctgcaaa
      721 aaggccagcg agccagggcc aagcacctct atcttgctga aagaggtttc atcaaacatg
      781 gctacgcgct ctcgtgtagc ctggtgttca gctcctatgg cgggcgacca gtagcggctg
      841 gcccaactgt atggcacggg ccatccctgg tcctccgcca gagcttcgtt cgaggcgaac
      901 cagttggggc gctcccagcc agctttctcg ccaaagaccg cgccaagttc ttcaaggcga
      961 taatagacgg gactgagtcg cagtttacgc gcgctgctgc gttctcgcat gggatagctg
     1021 atatcataat acgtggcgta gatttcgttg acgcgcgtgg caatgtagcg acggctggcc
     1081 gtgtacgcgc caaagcgccg gatatccatt tcccacagat caaggctggg ttcaccgtca
     1141 actatccact ccgctatcat cttgcctaca ccacctgcgc cggaaacccc gtgcgcgcag
     1201 aagccacagg cagcccagaa gcccttcaca tccggcgact cacccaggat aaactcgcca
     1261 tctggcgtga acgactcgag cccattcacc cgcttcacca gctcgacatc tcgcagcgag
     1321 gggacacgcg ctatgcatcc ctgcattaag ctctcgaagc gcggcgggtt gaagctctgt
     1381 accgtgggat gcgagttatc aggaatagca tcaacatcga aggcggccgg gtcatgctcg
     1441 tatccaccca gcaccatact gttcccatct tgccggaaat agaccaggtt atcggggtct
     1501 cgcaggttag ggatgcttgt ctctccagcc agttcagcca tcggaccggt cacgacatat
     1561 tgatgctgca taggaatgag tggaattgag atgccggcca gacggccgat gcgtggcgac
     1621 cagatgccag ccgccgcgat tactatttct gtgcgaatgc ggccacgcga ggtgttgatc
     1681 gcctgcacgc gaccattcac aacctcgatc cctgtgacgc gcgtattggc gtagaaggtt
     1741 gcgccgcgct gctgtgctat ggttgctaag gcctgcgcca ggacgtgcgc tttggcggaa
     1801 ccatccgtgg gcagaaacaa ggcgccttcg actccttcca ggctcatcat cggaaacatg
     1861 tcttttgctt cgctggcgct gataagctcc gcctccaatc cagcgctttt ggagaatccc
     1921 acctgccttt tgagctcctc catgcgttct ttcgatgaag ccaggcgtag gctccccact
     1981 tcgaagtaac cgggattgcc atctaccttg agttgcttat acagcgaaat gctagagacc
     2041 agcatcttcg tcaggctgat tgacgagcgc agttgaccaa caaggccggg cgcgtgcgat
     2101 gtcgtgccgc tgctgagcgt cccttgctcg aggacaacaa tgtcacgcca ccctcgttca
     2161 gtcagatggt atgcgatgct ggaaccagcg atgccgctac cgataatgac aaccctggct
     2221 tgatcgtgca tggataagtc tccttgattg aaaaatagtt tttgacgata tggcccctac
     2281 actttcacat ctttcaacca ctgctcgaaa ggcgatgttt cgacatttcg taaaaagcgg
     2341 ttcagatgat gatgcgcata ctcccggtaa tcaaaatcaa gttctgaaat gcccatttgt
     2401 aaaaagcccc agaatgactc gcgcaaatcg gagccgagcc gcatcaggtg caaatgtgcc
     2461 aactccgcgc tgcgcacctc gccaaaatag taatgcagca gcagttcgca ctgctcttcg
     2521 ttcaatgact ggttgacggc aaagttgcca agatcgaaaa atatgtcacc catgccggcg
     2581 tactcccaat cgagaatacg gatggtatgg ccatcgtcaa tgaaattgct ggcaagcagg
     2641 tcattatggc agggttttgt atacgctgtt ccactttgag caagcgcttg ctcgatcctg
     2701 gccatcaact caaaaacctg gggaagtgta tcaggaaacg agacgccgta ttctagtgcc
     2761 aatgtgtgat agttgcgcac cgtctcgaaa ggtgaaaagg cgcccgggaa gtctggccct
     2821 gcgtgatagc gtttcatgga atggacgatg cggcgcagag tttcgtcctg cgcggccagt
     2881 tcaggtgaga tgcctgtacc aacgatgaag cgggtcacca gcacatcttc gccagcaaga
     2941 aagtgcagga cctccgcccc aac
//
LOCUS       DASZAK010001360         4689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_34962, whole genome shotgun sequence.
ACCESSION   DASZAK010001360 DASZAK010000000
VERSION     DASZAK010001360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 4689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4689
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34962"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1084
                     /locus_tag="VGT44_23785"
     CDS             170..1084
                     /locus_tag="VGT44_23785"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV2583896.1"
                     /translation="MTQHNSKQQMKVGVIMDISEGSLEGRTPSFRDLQGMAQVAEQVG
                     LDSLWLADHLIYRFPGQDDSAPWEALTMLSALAAVTARATIGTLVVSTSFRPPAMVAK
                     MADAIDDISAGRFVLGLGAGWHQPEYEAFGYPFDHLASRFDEALQIIVPLLREGHVDF
                     QGKYYEVRNCVLRPRGPTPGGPRILIGARRPRMLRLVAQYADAWNTAWHTDPAVVKER
                     YEPLLQACADVGRDPAEIELTVGTLVRLQPARDDEQGQAISGAPEEIANRLQAFASVG
                     ASHLIVSLDEVTPASIAQLGRVVELMRG"
     gene            1214..1717
                     /locus_tag="VGT44_23790"
     CDS             1214..1717
                     /locus_tag="VGT44_23790"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEV2583897.1"
                     /translation="MAKRVTVTTELTTEELHERYRNAANAVERTHWHILWLIKEGRTP
                     DEVALMLGYTARWIRTIIQRWNRAGELAIRDQRRHQKGAPPLLSLEQQKELDQALDQP
                     PADGGLWSGPKVAAWMAQRLGREVDPRRGWDYLQRLGRSTRVPRPQHEESNQATQQAF
                     KKTPRGG"
     gene            complement(1692..2087)
                     /locus_tag="VGT44_23795"
     CDS             complement(1692..2087)
                     /locus_tag="VGT44_23795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583898.1"
                     /translation="MGWQKEQMKPFWNYEITTLVPARLVQNQKNLLVRPSALFLCEGG
                     QSEGKGCGIDRWQEQPGGLAALWLHKPIEIHPLIAGSDYGPNPAPFARPDPAQDRFEA
                     NAVLVLTPHFNAGLGIRLAQLLNLLGEFF"
     gene            complement(2289..3032)
                     /locus_tag="VGT44_23800"
     CDS             complement(2289..3032)
                     /locus_tag="VGT44_23800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583899.1"
                     /translation="MILRSLPRRGYLLLVWCLASWLLVACAGFGIGQNSIDDPTPVPV
                     TLRDPAPGQLTLALSITMDAAYDPSMRDTTTLEFQFLSHNQNVLFTGHEQFSCNGQTF
                     DLHTLPIRLMITQKTSALAGQTLRCVYSVGGLSTTLNFTVPHPPRILSPQDQASLARG
                     PNLLLKYDPGDGQIQTILALGVRSKASAQLDTPAPHEATLDTSSFPAGPGGIILTETP
                     NPPVAQSGMPFAQILTGGVANAQITVNWR"
     gene            3558..>4689
                     /locus_tag="VGT44_23805"
     CDS             3558..>4689
                     /locus_tag="VGT44_23805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2583900.1"
                     /translation="MSSVYCHHCGAANPAENAVCFACEHVLGMTEEQELEQEQAPLLR
                     ERYRLRAQVGTGGFGAVYRAEDTTQDDAIVAIKQINLAGLSAQEKIEATDGFYREVRL
                     LSGLKHPNLPTIHDSFTDPEHWYIVMDFIDGETLETYLAKRFSGQQEPGLPLSETLDL
                     GLQLCTVLDYLHTRQPPIIFRDLKPANIMRTPAGQVYLIDFGIARHFIPGKPKDTIPF
                     GSPGYAPPEQYGKAQTTPQADIYSLGALLHQALSGDDPAESPFRFAPLRLYGTIVIAD
                     LNELIQRMVQVDASQRPASAAEVKELLQRLADEQAKSEPRVWRPPETSLPLPPHYTTY
                     TGWHSGAAQQQVAQPVQPQHILNRRRFVRTVLGIGGTLVVGYA"
BASE COUNT          939 a         1381 c         1413 g          956 t
ORIGIN      
        1 gtcccataga cgttcaccga aggcgcctcc cccgctgtca tgctgagcgc agcgaagcat
       61 ctcacggcca atcacgatag acccttcgct gcgctcaggg tgacactgcc gaaggtggtg
      121 gtggaaaatc atcatcgcgt ccaaacgaca ttgacgaaag ggaaaagctt tgactcaaca
      181 caactccaaa cagcagatga aggttggtgt catcatggat atctcggagg gttcgctgga
      241 aggcaggacg ccatccttcc gcgatctaca gggcatggcg caggtggcgg aacaggtcgg
      301 cctggactcg ctctggctgg cggaccatct catctatcgc ttccctggcc aggatgattc
      361 cgcgccctgg gaagcgctga cgatgctcag cgcgctggct gccgtgaccg cgcgtgccac
      421 aattggtacg ctcgtcgttt ctacctcgtt tcgtccaccg gcgatggtgg cgaaaatggc
      481 cgatgccatc gatgatatca gcgcgggccg cttcgtgctg ggtcttgggg cgggctggca
      541 tcagcctgaa tacgaggctt ttggctatcc attcgatcac cttgccagcc gcttcgacga
      601 ggcgctgcaa atcattgtgc ctttgctgcg agaaggccat gttgatttcc aggggaaata
      661 ttacgaggtg cgtaactgcg tcctgcgccc gcgcggccct acgccgggcg ggccgcgtat
      721 tctgatcggc gcgcgccgcc cgcgtatgtt gcgtctcgtc gcgcaatatg ccgatgcctg
      781 gaacaccgcc tggcataccg atcctgccgt tgtgaaggag cgctacgagc cgctcttaca
      841 agcctgcgcc gatgttgggc gcgatcccgc cgagatcgag ttgacggtcg gcaccctggt
      901 gcgcctccaa ccggccagag acgacgaaca gggacaggca atctcaggcg cgcccgaaga
      961 aatcgccaac cgcctgcaag ctttcgccag cgtgggcgcc tcccacctga tcgtctcgct
     1021 cgatgaggtg acgcccgcca gcatcgcaca gctcggtcgc gtcgtcgaac tgatgcgcgg
     1081 gtgatggtgg ttggcaattt tatctcggaa tgtgtgtagg gacaggcgca aggcccccgc
     1141 cacgccgctc tgcctccccc tgtccctacg catgtgtcat atcaaatccg tgtaggtagt
     1201 gtataatgtg gacatggcaa aacgagtgac cgtgaccacc gaattgacaa ctgaagagct
     1261 gcatgaacgc tatcgaaatg ccgccaacgc cgttgagcgg acgcattggc acattctctg
     1321 gctgatcaag gagggccgca cgccagatga ggtcgcgttg atgcttggct acactgccag
     1381 atggattcgc accattatcc agcgatggaa tcgagctggc gaactggcaa tccgtgacca
     1441 gcgtcgccac cagaaaggtg ctccccctct gctgagcctg gagcagcaga aggagttaga
     1501 tcaggcgtta gatcagcctc ctgctgatgg tgggctgtgg agcggcccca aagtcgcggc
     1561 ctggatggct cagcgcttgg gccgcgaagt ggacccgcgt cgcggctggg attacctgca
     1621 acggcttggc agaagcacac gcgtgccccg cccgcagcat gaggagagca accaggccac
     1681 ccagcaggct tttaaaaaaa ctccccgagg aggttgagca gctgcgccag gcgtatcccg
     1741 agaccagcgt tgaagtgtgg agtcaggacg agcaccgcat tggcctcaaa ccgatcttgc
     1801 gccgggtctg ggcgcgcaaa gggagccggg ttcgggccgt agtccgaccc agctatcaat
     1861 ggatgtatct ctatgggttt gtggagccac agagcggcaa gacctcctgg ttgctcctgc
     1921 caacggtcaa taccgcagcc ttttccctcg ctttggccgc cttcgcacag gaacagggcg
     1981 ctgggccgca caagcagatt cttctggttc tggaccaggc gggctggcac aagagtggtg
     2041 atctcgtagt tccagaaggg cttcatctgc tctttttgcc atcccattca cccgagctgc
     2101 agcctgcaga acgtttgtgg cctctctcca atgagccgct tgccaatcgg gtcttcagct
     2161 cgcttgacga gttagaagac gctcaagctg cccgctgctg ttggctccaa gctcatcctg
     2221 agatcattcg tgggcgaacc tcctttcatt ggtggccttc tttgtgcact ccttaaacga
     2281 attcactatt agcgccaatt tacggtgatc tgcgcgttcg cgacaccgcc agttaagatc
     2341 tgcgcgaagg gcatgccgga ctgagctacg ggcggattgg gcgtttctgt gaggataatc
     2401 ccacccggac ctgctggaaa cgagctggta tcgagggttg cttcatgggg cgcgggcgtg
     2461 tcgagttgcg cgctggcctt gctccgaacc cccagggcta agatagtctg gatctgccca
     2521 tcgccgggat cgtacttgag aagcagattg ggaccgcgcg ccaggcttgc ctggtcctgt
     2581 ggggagagga tgcgtggcgg gtgtggcacg gtaaagttca gcgtggtaga cagaccgccc
     2641 acgctataga cgcagcgtag tgtttgccca gccagcgcgc ttgttttctg cgtgatcatt
     2701 agccttatgg gcaacgtgtg cagatcgaag gtctgaccat tgcaggagaa ttgttcgtgt
     2761 cccgtgaaaa gaacgttctg gttgtgtgaa agaaactgga actcgagcgt tgtggtgtcg
     2821 cgcatggagg ggtcataggc agcgtccata gtgatagaga gcgcgagcgt caactggcct
     2881 ggagccggat cacgcagcgt caccggcacc ggggtggggt cgtcgatgga gttctgacca
     2941 atgccgaagc cggcgcaggc caccagcagc cagctcgcca ggcaccagac caggagcaaa
     3001 tatccccgcc tgggaagaga tcgcaaaatc atagacagca cctgcctttt cctgcagccg
     3061 cggcactgca accgacaatc gcggcactgt ttgttcttac tcaagagacg tccagtgtga
     3121 gcgttttgtt cccgatgagc attgtttcag ccagaaaaaa ggcgtgttta ttgttacccc
     3181 ctggaaatgt ctttcttcca tgatttgctt cccaggaagc gcccagcgcg accaggatgc
     3241 ctccaagcac ctcgccagct cgattccatc gctggacaat gggacgaatc cacctggtcg
     3301 taggcgccac ccgcgtcctg gagtgtggcc ctccttcatg agccacagga tgtgccaatg
     3361 ggtccgccga gcgcgcgtcg gttgcgcttc agttgtcaat tcggtggtca cagtgactcg
     3421 ttttgccatg cccacattat acactcttta aacggatttg atatcacaca ttaacgagat
     3481 aaaagcagcg tcaaaagcgt attgttactt gggagtggga agctcaggga acaagcaacg
     3541 ctggaggagt ctctgctatg tcttcggtct actgtcatca ttgcggcgcg gcgaatcctg
     3601 ccgagaacgc cgtctgtttt gcctgcgagc atgtgctggg catgacggag gagcaggaac
     3661 tggagcagga gcaggctcca ttactgcgcg aacgatatcg cttgcgcgcg caggtgggaa
     3721 cgggcggctt cggcgcggta taccgggcgg aggatacgac ccaggacgac gcgattgtgg
     3781 cgatcaagca gatcaacctg gcagggcttt cggcccagga gaagatcgaa gcgaccgatg
     3841 ggttctaccg cgaggtgcgc ctgctctctg gtttgaagca cccgaatctg ccaaccattc
     3901 acgactcgtt taccgacccg gaacactggt atatcgtgat ggatttcatt gatggcgaga
     3961 ccctggaaac ctacctggcg aagcgcttct ccggccagca ggagccgggc ctgccgttga
     4021 gcgagacgct tgacctgggc ctgcaattgt gtacggtgct ggactacttg cacacacgcc
     4081 agcctcctat catcttccgc gacctcaagc cggccaacat tatgcgcacc cctgccgggc
     4141 aggtctacct gatcgatttt ggcatcgcgc gccatttcat cccaggcaag ccgaaagaca
     4201 ctatcccctt tggctcgccc ggttacgccc cgccggaaca atacggcaag gcgcagacga
     4261 cgccgcaggc ggatatctac agcctgggcg cgttactgca ccaggcgctc agcggcgatg
     4321 atcccgccga atcgccattt cgcttcgctc ctctgcggtt gtatggtacg atcgtcatag
     4381 ctgatctgaa cgaactcatc cagcgcatgg tgcaggtcga tgccagccag cgacccgcca
     4441 gcgctgccga ggtgaaggag ttactccagc gcctggccga cgaacaggcg aaatccgagc
     4501 cgcgcgtctg gcggccaccc gaaacatcgt tgccgctgcc tccacactat acgacttata
     4561 ctggctggca tagcggcgct gcccagcagc aagtcgcgca gccggtacag ccacagcaca
     4621 tcctcaaccg gcgtcgcttc gtcaggactg tgctgggcat tggtgggacg ctggttgtgg
     4681 gatatgccg
//
LOCUS       DASZAK010001361         1781 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_35066, whole genome shotgun sequence.
ACCESSION   DASZAK010001361 DASZAK010000000
VERSION     DASZAK010001361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35066"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..866)
                     /locus_tag="VGT44_23810"
     CDS             complement(186..866)
                     /locus_tag="VGT44_23810"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV2583901.1"
                     /translation="MTHEILRCAQDDRTDGGQGPSSSLQQDGSPLVVLDAGCGEGYYL
                     CRLQAELAARDMQAICLGLDISKEAVRLATARCREAFFVVADLKERLPIIENSVHILL
                     NIFAPRNPPAFARVIAPGGLLLVVIPSPTHLLQLRTALHLLQIEEEKQRHVIAQFTAQ
                     DMFRLEKTTPVSYELHLQGEAVRQLVTMTPNYWHLTEQMSQAIAQLEEIRTEVACLCL
                     AFRRQEQA"
     gene            complement(1198..>1781)
                     /locus_tag="VGT44_23815"
     CDS             complement(1198..>1781)
                     /locus_tag="VGT44_23815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583902.1"
                     /translation="GSYNPSTHTFKEFPTPSANSQPYGITVDSHNNVWFAENNSSVAR
                     IGEYTAQGKMNEYTIRSNPPGGLTPHMITVDHNGNIWWTEGWVGMIGELQVSQAVPGT
                     NKGVTEYSYPAPCGSCGGTHASGISVDTNGNVWFSDALQDIIASFPVSGNGSFTEYNV
                     PTQNGHPHDGLNVDSKNRVWFTEEFANKLGVGQ"
BASE COUNT          347 a          473 c          557 g          404 t
ORIGIN      
        1 tggtcggcct ggcctgccgc tctgccctgc gccggggacg cacgttgggg cgcgatgagc
       61 agcgcgcgct gatccacgcg ctggccattg tggccgcccc agcggtctgc ccgcacggca
      121 gcccgattgt gctgcactat agccggaagt tcctggtgga ccggttcgac tggtgagagg
      181 gtaggctacg cctgctcctg tcggcgaaac gccaggcaca gacaggcaac ctccgttcgt
      241 atctcctcta attgagcgat ggcctgggac atctgctcgg tcaagtgcca gtagttgggt
      301 gtcatggtca cgagttggcg aacggcttct ccttgtaagt gcagctcgta ggacacgggc
      361 gtcgtttttt ccaggcggaa catgtcctgc gcggtgaatt gtgcgatcac gtgacgctgt
      421 ttctcctcct caatttgtaa gagatgcagc gcggtacgca gttgtaggag gtgggttggc
      481 gagggaataa ccaccagcag cagaccgcca ggggctatga cgcgggcgaa tgctggcgga
      541 ttgcgcggag cgaagatatt caagaggata tgcaccgaat tttcgatgat gggtaggcgt
      601 tctttgagat cggcaacgac aaaaaacgct tctcgacagc gcgcggtggc caggcgcacg
      661 gcttctttcg agatatccag gccgaggcag atcgcctgca tgtctcgcgc tgccagctcg
      721 gcctgcaggc ggcagaggta gtagccttcg ccacaaccgg catcaagtac gaccagcggc
      781 gacccgtctt gctgcagcga tgatgaaggt ccctgacccc catcggttct gtcatcctga
      841 gcgcagcgaa ggatctcatg cgtcaaagac cgagatcctt cgctgcgctc aggatgacaa
      901 cctatgaacg attctggttg tgaaacttca ttatcggcga gatgcgcgct gacgatctca
      961 ttcaccgcgt ctgatagggg gagaaagtgg ccttgcgcaa aaatggtttg gcgcgcgagc
     1021 agcatctccc tggtatcgcc ggcggccttt ttcagcagga aattgacgta gcctttcctg
     1081 gcgatatcga atgagtgcgc gttcgtacat gtcagtgcgc gctccgtagg gataaacggt
     1141 gttgcgcaaa ttggacatgc gagatatggt atgtgcggca aagccattga cttgttccta
     1201 ttgtcccacc cccagtttat tcgcgaactc ctcggtgaac cagacgcggt ttttggagtc
     1261 tacattcaga ccgtcgtgag ggtgcccgtt ctgcgtaggt acattatact cggtgaagga
     1321 gccattgcca ctgacgggga acgaggcgat gatgtcttgc aaggcatcgc tgaaccagac
     1381 gttgccgttc gtatcgacac tgatgccgga ggcgtgggtg ccgccgcaac ttccgcaggg
     1441 cgcgggatag ctatattctg tcacgccctt gttggtgcca ggcacggcct ggctcacctg
     1501 taactcgccg atcatgccaa cccagccttc tgtccaccag atattgccgt tgtgatcgac
     1561 ggtaatcatg tggggagtca ggccgccggg tgggttggaa cgaatggtgt attcgttcat
     1621 ttttccttgc gcggtatatt cgccgatgcg cgcaaccgag gagttatttt cggcgaacca
     1681 gacattgttg tgcgagtcaa cggtgatacc gtagggctga ctgttggccg atggcgtggg
     1741 aaattccttg aaggtatgcg tggaaggatt gtagctgcca a
//
LOCUS       DASZAK010001362         8176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_318396, whole genome shotgun sequence.
ACCESSION   DASZAK010001362 DASZAK010000000
VERSION     DASZAK010001362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8176
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318396"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..868
                     /gene="fabG"
                     /locus_tag="VGT44_23820"
     CDS             104..868
                     /gene="fabG"
                     /locus_tag="VGT44_23820"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV2583903.1"
                     /translation="MADHELPLAGKVALVTGSSQGIGRATALRLAQSGADIVINYRSN
                     VSAAEDTRSSIEAMGRRCIAVQADVSQEEDVYRLFSEANTAMGVASILVNNAGTTRDK
                     LIIQMSLADFEYVLNTNLRSAFLCTKAALRGMMRARWGRIVNISSPSGLLGNAGQANY
                     SASKAAIVALTISTAREMASRNITANAIAPGFIPTELTSTLTDQQRQLMLEATPLGRF
                     GTAEEIAAAIHFLCLPESGYITGQILCVDGGMAMHI"
     gene            942..1808
                     /locus_tag="VGT44_23825"
     CDS             942..1808
                     /locus_tag="VGT44_23825"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV2583904.1"
                     /translation="MSLVETELTNRFTFEAFASHEFYTEINRSLVRQALAPFARFPGA
                     APLTIVDMACGTGAVTRLIAEEMQRQRQRARIIGVDPSAEALRRAAKWMGTLESNGIS
                     TEFIQGDAEDLPRLVKNANAAFFCNAIHLLPDKLAAFRLMSSILAPRGVFACNSGFYQ
                     GTYIEGTDRFYRLWTNKAVRWLRKEHPEVRLSREAKAMAMQWMRADEYVDQLRRGGFD
                     HIKATQEVGMISIDAWRDIGHYWLFIEGALPGVPLEWGAAALEKAVYQAGEEMGISAT
                     PRNWLQLIASKE"
     gene            1932..2729
                     /locus_tag="VGT44_23830"
     CDS             1932..2729
                     /locus_tag="VGT44_23830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583905.1"
                     /translation="MQWAIASASFLASAVEFVEAFTIVLVVGVTINWRSSLVGAFAAA
                     ATLAVIVGTLGVAIVQLVPINALRLFVGIILILFGLKWLKKSILRYSGLKSLHDEEAI
                     FEEKMAEIRARGETIEPRLQPFGVALSYKAVLLEGLEVAFIVITFGSSISTVANSNGI
                     ASAALGATAAGILVILAGWLIKAPLTKVPENTLKFVVGIMLTSFGTFWSGEGFGYTWP
                     LSDLFIPILAAIYLLATFALVMWLKQVKRHQLAASQSQETPISSLRA"
     gene            2750..2953
                     /locus_tag="VGT44_23835"
     CDS             2750..2953
                     /locus_tag="VGT44_23835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583906.1"
                     /translation="MNTIKAIYNFIVGDIIILVGVAIVAIALGIINAVSAFAALRPAT
                     GIVLVLIVLIVLFATLYREAHGR"
     gene            3094..4017
                     /locus_tag="VGT44_23840"
     CDS             3094..4017
                     /locus_tag="VGT44_23840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV2583907.1"
                     /translation="MIVKEHRFRQRIIDELGIKPITITRWIEGRSEPRQQNFRQLINA
                     LPEFRENFYELLGEEFTEAAIVSASDASKAIPSEFFARVMSERTSTQRTIRFWSLCYL
                     ILQQAIGQLDPDQFGMAITVVQCMKSSSSPVIRSLRETAAYGTVPWQSNMEQKGMFLG
                     AESLAGYCVSSCRGVENNDVRDRSNPLPAHQVPDELSAAAYPILLSGKVAGCLLVSST
                     QPNFFLSQFRLNLIHNYANLISLLFEPEQFFDLDKIRLQIMPLHIQQKAYFSDFRHRV
                     INAMRIHQLSNMDAEQLVWEELEKNFLAFEQ"
     gene            4057..4881
                     /locus_tag="VGT44_23845"
     CDS             4057..4881
                     /locus_tag="VGT44_23845"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HEV2583908.1"
                     /translation="MFDDFRGPLQDRWRQTCIGTGSLLNSETTMRMMLAATRQGDYSD
                     AQIDDYGRLPRSQYPWRPPVRMEVRARSGLPGADPAISVEQPGTLRGTAGFGFWNYPF
                     SVRGDVLMLPEAVWFFYASPPSNMALVPGVPGWGWKAQVVHSLRPGAAAAALPLAITA
                     ALGRLTGNSRPAGRWMQRLTGASEALLHVEMAEWHTYTLDWQPQEALFRVDGETVLRV
                     ANPPTRPLGFVAWLDNQYAIATPRGVLRFGALDCGPQWIELDSVRIDTLDLTSRPG"
     gene            complement(4862..5464)
                     /locus_tag="VGT44_23850"
     CDS             complement(4862..5464)
                     /locus_tag="VGT44_23850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEV2583909.1"
                     /translation="MSSSLLVPTVIETTGRGERAYDIFSRLLRERIIMINGQIEESMS
                     SLVVAQLLWLASESAEREINLYISSPGGSVQAGLAIYDTMRHLPCPVSTTCVGYTASF
                     GTILLMAGDRGLRRSQPHARIHMHQPLIPGGLGGQATDIDIMAREILHTRDVINEIIQ
                     RHTGQPLERIRRDTDRDFYMSAEEALEYGVIDEVLTLAEK"
     gene            complement(5492..5767)
                     /locus_tag="VGT44_23855"
     CDS             complement(5492..5767)
                     /locus_tag="VGT44_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583910.1"
                     /translation="MIYREMRSPVGGLLSEQKDVKMYPLVPMVYEHVPAEPLSWEYRI
                     LSIDLREQDMPTVEKLNELGGQGWLLVGALNTDQRRVNYYFVRQKQA"
     gene            6011..6406
                     /locus_tag="VGT44_23860"
     CDS             6011..6406
                     /locus_tag="VGT44_23860"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV2583911.1"
                     /translation="MADLQEIMGRVIRRERQDRQLTIKELGDKAGLSEIYVGEIERGQ
                     KYPSSKVLESLADALELDMADLLELMADEIRGERMPQTSAIGFVLPTAPGQPHRVTVK
                     RIVNMLPEGEVESIADFSAFLLSKHFKPS"
     gene            complement(6437..6775)
                     /locus_tag="VGT44_23865"
     CDS             complement(6437..6775)
                     /locus_tag="VGT44_23865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007915336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HEV2583912.1"
                     /translation="MVITLTALIIWLLIAALVGVIGELLARRRAPFGIVGAMVLGFIA
                     IFLVVGVLGIHFAGDPTLNGVPIFTSILVAAIVVAIWSAFAHRRAYGYYSRYYRRGSY
                     VRRPRRYGPF"
     gene            complement(6928..7950)
                     /locus_tag="VGT44_23870"
     CDS             complement(6928..7950)
                     /locus_tag="VGT44_23870"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV2583913.1"
                     /translation="MPSIKDVAKAAGVSTATVSRVLSNGEHVRQEVRKRVMDAVEKLE
                     YRPNLLARSLRSQQTTTIGLIVSDIRNPFFTSVSRAVEDTAYEQGYSLFLCNTDENPE
                     KEAIYLNLMRDESVAGVIFSPTRQTAADFASFNFSFPIVIVDRFVAGADVDMVLLDNI
                     DAAFRLTTHLLEHGYRRIAALCGDMSATGRERYSGYEKSLRAQDLAPSADLVKFVPPR
                     AEAGYAATLKLLDLPQPPDALLATNSLLASGALQAIRERELAMPADIALVTFDETSWA
                     SLVQPPVTLIAQPTYEIGKTATELLLQRIADPSRSTRQVILKGQLLERGSSAARQSTP
                     QMDDAH"
BASE COUNT         1824 a         2279 c         2271 g         1802 t
ORIGIN      
        1 gattgatcgc gtcctcatct cttatcgagc aaggcatccc ggacgcgatc aatcgcgtcc
       61 ctacgaccat taaagcaaga cgaccgaggg aaagtaggag actatggcag accacgagtt
      121 gcccctggcg ggaaaagtcg ccctcgtgac cggcagcagc cagggaatcg ggcgggcaac
      181 cgcgcttcgc ctggcgcaat cgggcgcgga tatcgttatc aactatcgca gcaatgtcag
      241 cgccgcggag gatacgcgct ccagtatcga ggcgatgggc aggcgctgca tcgcagttca
      301 ggccgacgtg agccaggaag aagatgtata caggttattt agcgaggcga acacagccat
      361 gggcgtcgcg tcaatcctgg tcaacaacgc cgggaccacc cgcgacaagc tgatcataca
      421 aatgtccctg gccgattttg aatacgttct gaacactaac ttgcgttcag cctttctctg
      481 caccaaagct gcgctgcgtg gcatgatgcg agcgcgctgg ggacgcatcg tcaatatctc
      541 atcgccatct ggcctgcttg gtaacgcggg ccaggccaac tacagcgcct ctaaagcggc
      601 cattgtcgct ctgacgatca gcaccgcgcg agaaatggcg agccgcaaca taacagccaa
      661 cgccatcgcg cccggcttta tcccgaccga gctgacctca acactgaccg atcagcagcg
      721 ccaattaatg cttgaagcga caccgcttgg gcgcttcggc acagcggaag aaatcgccgc
      781 ggcgatccat tttctttgtc tgccggagtc aggttatatt accgggcaaa ttttgtgtgt
      841 ggacggcggc atggccatgc acatttgaac attcatgaaa caaatcggat gcagtttcgc
      901 tctaagtgta aaatgccaga tcatcaaagg agactgcatc tatgagctta gtcgaaaccg
      961 aattaaccaa tcgcttcacc tttgaagcct tcgcttccca cgagttttat acagagatca
     1021 accgttcgct ggtgcgccag gcgcttgccc catttgcgcg tttcccaggc gcggcgccgc
     1081 tcaccattgt tgacatggcc tgcggcaccg gcgctgtcac acgcctgatc gcggaagaaa
     1141 tgcagcgcca gcgccagcgc gcgcgcatca tcggcgtcga tccctccgcc gaggcgctgc
     1201 gcagggcggc aaagtggatg gggacactgg agagcaatgg gatctccacc gaatttatcc
     1261 agggtgacgc cgaagatttg ccgcgattgg taaagaacgc caacgcggcc tttttctgca
     1321 atgccattca cctgctcccc gataaacttg ccgccttccg gctaatgtcc agcattctcg
     1381 cgccacgtgg tgtattcgct tgcaacagcg gcttctatca aggcacctat attgagggaa
     1441 cagaccgttt ttaccgcctc tggacaaaca aagcggtgcg ctggctgcgc aaggaacacc
     1501 ctgaggtgcg cctgtcgcgc gaggccaagg ccatggcgat gcagtggatg cgggcggacg
     1561 aatacgtcga tcaactgcgg cgcggaggct tcgatcatat caaagccacg caggaagtag
     1621 gtatgatctc gatcgatgcc tggcgtgata tcggacacta ttggctcttc atcgaaggcg
     1681 ccttgccggg cgtcccgctg gagtggggcg cggcagccct ggagaaagct gtctaccagg
     1741 ccggggaaga aatgggcatc agcgcaacac ctcgcaactg gctgcaattg atcgcatcga
     1801 aagaataggg cgaactcgct gcattaacac tcgctttaca ttcgtgtgct acaataggat
     1861 agctggcgca tgagtgttac gacctcatgc tgcattttca ccaggttttt cccggaaaag
     1921 gagtgtagtt catgcaatgg gcaatcgcct cagcatcctt cctggcctct gctgtggaat
     1981 ttgtagaagc ctttacgatt gtgctggtgg tcggcgtgac catcaactgg cgcagttcgc
     2041 ttgttggagc gtttgcggct gccgctaccc tggcagtgat cgttggtacg ctgggggtgg
     2101 cgattgtgca actcgttccc atcaatgccc tgcgattatt cgttggtatt atcttaattc
     2161 tcttcggcct gaaatggctc aagaagtcga tcttgcgcta tagcggcttg aagtcgctgc
     2221 acgacgagga agctatcttc gaggaaaaga tggcggaaat tcgcgcgcgc ggcgaaacaa
     2281 tcgagccgcg tttgcagcca ttcggcgtgg cgctctccta caaagcggtc ctgcttgaag
     2341 ggttggaagt cgccttcatc gtcatcacgt tcggcagcag catctccacc gtggccaact
     2401 ccaacggtat tgcctcggct gcgcttgggg cgaccgccgc cggtatcctg gtgatcctgg
     2461 ctggatggtt gattaaagcg ccactgacaa aggttcccga aaatacgctt aaatttgtgg
     2521 tcggtatcat gcttacttcc tttggcacct tttggagtgg cgaagggttt ggatacacct
     2581 ggccgctttc tgatctcttc attcccatcc tggcagctat ttacctgctc gcaaccttcg
     2641 cgttggtcat gtggttgaag caggtcaagc gccaccagct tgccgcatcg caatcacagg
     2701 aaacacccat cagcagtctg cgcgcctagt ataactggag agatcgttca tgaataccat
     2761 caaggccatt tacaacttca tcgtcggcga cattattatc ctggtcggcg tcgccatcgt
     2821 cgctatcgcg ctgggtatca tcaacgccgt cagcgcattt gccgctctgc gcccggcgac
     2881 cggcatagtg ctggtactca tcgtcctcat tgtgctgttt gccacgctct atcgcgaggc
     2941 gcacggcaga tagtagcgct tctcagttgt tgacgagtat gcaaggaact gctatgattt
     3001 gggccaggag atgcagtatt gcctattttc ggcaattgag gggaaaacag tatggagttc
     3061 gaggacgcga aagattggcg cgaactactc gccatgattg tcaaagaaca tcgctttcgg
     3121 cagcgtatta tcgatgagtt gggcataaag ccgatcacca ttacgcgctg gattgaggga
     3181 cgttcagagc cacgccaaca aaatttccgg cagctgatta acgccttacc tgagttccgg
     3241 gaaaactttt atgagctgct gggtgaagaa tttactgaag ccgccatcgt cagcgcaagt
     3301 gatgcctcta aagcgatccc cagcgaattc tttgccaggg tgatgtccga gcgtacaagc
     3361 acccagagaa ccatacgttt ctggtcgttg tgttatttaa ttctccaaca agcgattggc
     3421 cagcttgatc cagaccagtt tggcatggct attaccgtcg tgcagtgcat gaaatcatcg
     3481 tccagtccgg tgatccgcag cctgcgcgaa acagcggctt atggcacagt tccctggcaa
     3541 agcaatatgg aacaaaaggg catgttcctg ggggcagagt cgctggcggg ctattgcgtt
     3601 tcttcgtgca gaggcgttga aaacaacgat gtacgggacc gctcgaatcc tcttccggcc
     3661 caccaggttc ccgacgaatt gagcgctgct gcctatccca tcctgctctc cgggaaggtc
     3721 gccggttgcc tgctggtctc cagcacacag ccaaatttct ttctttcaca atttcgtctg
     3781 aatctgattc acaactatgc caatctgatt tcactccttt tcgaaccaga gcagtttttc
     3841 gacctcgata agattcggct tcagataatg cccttacaca tacaacagaa agcatacttt
     3901 agcgatttcc gtcatcgcgt tatcaatgct atgcgcatcc atcaactctc aaatatggat
     3961 gcggagcaat tggtctggga agagctggaa aagaattttc tggcttttga gcaatgagca
     4021 ggacccctgg cgtctggata gcctggtggt gccgccatgt ttgatgattt tcgcgggccg
     4081 ctccaggacc gttggcgcca gacctgcata ggcactggtt cgctgctcaa tagcgagacc
     4141 accatgcgta tgatgcttgc tgctacccgg cagggagact atagcgacgc gcaaattgat
     4201 gattatgggc gcttgccgcg ctcgcaatac ccctggagac cgccggtgcg aatggaggta
     4261 cgggcgcgct cagggctgcc tggcgctgat cctgcgatca gcgtagagca gcccggcacg
     4321 ctgcgcggca cagccggttt cggcttctgg aactatccct tctcggtgcg cggcgatgtg
     4381 ctgatgctgc cagaggctgt ctggttcttc tatgcctcgc ccccctcgaa tatggcgctc
     4441 gttcccggtg ttccgggctg gggttggaag gcgcaggttg tgcatagctt gcgcccaggt
     4501 gctgccgccg ccgcgctacc tctggcaatc acagcggcgt tgggtcgtct gaccggcaat
     4561 tcacgaccgg caggccgctg gatgcagcgt ttgaccgggg cgagcgaagc gctcctgcat
     4621 gtcgaaatgg ccgagtggca tacttacaca ttggattggc agccccagga agcgctcttt
     4681 cgcgtcgatg gtgaaacggt gctgcgcgtc gcaaacccgc caacgcgccc cctgggcttt
     4741 gtggcctggc tcgacaatca gtatgccatc gccaccccac gcggcgtact gcgcttcggc
     4801 gcgctggatt gcgggccgca gtggatcgag ctagactcag tcagaatcga taccctcgac
     4861 cttacttctc ggccagggtg agaacctcgt cgatgacgcc atattccagc gcctcttccg
     4921 cgctcatgta gaaatcgcgg tcggtatcac gcctgatgcg ctcgagtggc tggccggtgt
     4981 gacgctggat gatctcgttg atgacatcac gcgtgtgcag gatttcgcga gccatgatgt
     5041 cgatatcggt cgcctgcccg cccagaccgc cgggaatcaa tggctggtgc atgtggatac
     5101 gcgcatgcgg ctgggagcgg cgtaatccac gatcgcccgc catcaacagg atggtgccaa
     5161 agctggcggt atagcccacg caggtcgtgc tgacgggaca cggcagatgg cgcatcgtgt
     5221 cgtagatcgc caggccggcc tggaccgagc ctccgggcga actgatgtac aggttaatct
     5281 cacgctccgc gctttccgag gccagccaga gcaattgcgc caccaccagg ctgctcatgc
     5341 tctcttcgat ctggccatta atcatgatga tgcgctcgcg cagcaaacgt gaaaaaatgt
     5401 cataggcgcg ctcgccacgg cccgtggttt caatcaccgt gggaaccagt aaacttgacg
     5461 acattgctgt atttcctttc attgccggtg gttacgcctg tttctggcgc acaaagtagt
     5521 agtttacccg ccgctggtcg gtgttcagcg caccaactag cagccagccc tggccaccca
     5581 gttcgttcaa tttctcaaca gtaggcatat cctgttcgcg taaatcgata ctcagtatgc
     5641 ggtactccca actcagcggc tcagcaggca catgttcata caccattggg accagagggt
     5701 acatcttgac atccttttgt tcagacaata gccctccaac gggagagcgc atttctcgat
     5761 aaatcatgcg gaatgctcct ttgtagagta tctcgttgca tggcgaccgc aagggtcgcc
     5821 actacgataa cccgaataaa tcaaccaacg atgtaacgtt atcgtagtgg caacccttgc
     5881 ggtcgccatg caataggtta catccctacc ttgaatctac cacgcccata gcctataatg
     5941 gaattgagaa aacatgctct cagcatagga atacaaaaag ctatgccata agcatagaaa
     6001 ggatacacag gtggctgatt tacaggaaat tatggggcgg gtcattcgtc gcgagcgaca
     6061 ggaccgccag ttgaccatca aggaattggg cgataaggca gggctatcgg agatttacgt
     6121 cggggagata gagcgagggc agaaataccc ctcgtcgaaa gtgctggaaa gcctcgcaga
     6181 cgctctagaa ctggatatgg ccgacctgct ggagctgatg gcggatgaga ttcgcggcga
     6241 gcggatgccg cagaccagcg cgattggctt tgttttgcca accgcgccgg ggcagccaca
     6301 ccgcgtcacg gtcaagcgta tcgtcaacat gctgcccgaa ggggaagtgg aaagcattgc
     6361 agatttcagc gccttcctgc tctcgaaaca cttcaaacca tcgtgactga tctgcttgat
     6421 gatctgtagc tgcgcactag aacgggccat agcggcgcgg gcggcgcaca tagctgccgc
     6481 gacggtaata gcgcgaatag tatccgtaag cacgtcgatg ggcgaaggcg ctccaaattg
     6541 ctactacaat cgcggcgacc aggatcgagg tgaatattgg cacaccattc aatgtcggat
     6601 cgccagcaaa atggatgccc aggacaccga ccaccaggaa tatggcgatg aagccaagca
     6661 ccatggctcc aacgatgcca aagggcgcgc ggcgacgcgc aagcagttct ccgataacgc
     6721 caactagcgc ggcaatcaat aaccagatta tcaaggcagt caatgtgatg accattgtct
     6781 atcatctcct tcgataaaat cgttcggaca atccatgcga ccttcacaac tgaccgtgaa
     6841 acagaatcgc attccttcct catacacgca aatactggct gaacagttgc acgtacccat
     6901 agatccctac gaccgggagg gcaattatca atgggcatcg tccatttgag gtgtggactg
     6961 gcgagccgcg cttgagcctc gctcaagcaa ctgccctttc agaatgacct ggcgggtgga
     7021 gcgggaggga tcggcgatgc gttgtaagag caattcggtg gctgttttgc caatttcata
     7081 ggtgggctgt gcgatcaatg tcacaggtgg ttgaacgagg gaggcccagg aggtttcatc
     7141 gaaggtcacg agggcaatgt ccgcaggcat agctagctca cgctcgcgaa tagcctgcag
     7201 cgcgccggag gccagcaggc tgttggtggc caggagcgca tcgggcggct gtggcagatc
     7261 aagcagcttc agggtcgcag cataccctgc ctcggccctg ggcggcacaa attttactag
     7321 atctgcagat ggggccaggt cttgagcgcg cagggatttc tcgtagccgc tatagcgctc
     7381 cctacccgta gcgctcatat cgccacacag cgccgcgatg cgccggtagc catgctccag
     7441 caggtgcgtg gtcagccgaa aagcggcatc gatattatcc agtagcacca tatccacgtc
     7501 agcaccagca acaaagcggt caacgatgac tataggaaac gagaaattga aggaggcgaa
     7561 gtcggcggcg gtctgccgcg tgggcgagaa aatgacgccc gccacgcttt catctcgcat
     7621 caggttcagg taaatggctt ccttctctgg attttcgtcg gtattacaca aaaacaggct
     7681 atagccctgc tcataggcag tatcttcaac ggcgcggctg accgaggtga aaaagggatt
     7741 gcggatgtcg gagacgatca ggccaatggt ggtcgtctgc tgagaccgca ggctgcgcgc
     7801 cagcaaattc gggcggtact ccagcttctc aacggcatcc atgacgcgct tgcgtacctc
     7861 ctggcgcaca tgctccccat tggaaagcac gcgcgacacg gtcgccgtcg agaccccggc
     7921 cgctttcgcg acatctttga tactcggcat atagcctcct cctcgtggaa tctcaggcat
     7981 ttttcttgca ccatattgta atcgtttcat tgaagtataa caaattttgg tacacaatac
     8041 aagaattatt gtaatcgttt acgctacatg cagccttgat gatgtttcaa ctcacctggt
     8101 ttcggctcgt aattgcgaca tttaagcaca acgacaaaat tccttcccaa aacacttgac
     8161 aaatttattg taatcg
//
LOCUS       DASZAK010001363         3928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_140188, whole genome shotgun sequence.
ACCESSION   DASZAK010001363 DASZAK010000000
VERSION     DASZAK010001363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140188"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..741)
                     /locus_tag="VGT44_23875"
     CDS             complement(163..741)
                     /locus_tag="VGT44_23875"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEV2583914.1"
                     /translation="MPTRAEILDTLAASQTQVMAFFQGLSPEDLERPATASEVPGAAP
                     WRAKDHLAHLVKNERNIQQMLRCTLAGETRDVLLRLQYPEGMPMPGILGNLSALTPEE
                     EEQLGMAVASVNQAYVDAHRDDSLEMLAADYLAARQDTVDLLRQFTDDQLAAPVPTVV
                     GDATAGDLFAGRAGHAAEHMTSIEEGFRQGVC"
     gene            1015..1404
                     /locus_tag="VGT44_23880"
     CDS             1015..1404
                     /locus_tag="VGT44_23880"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV2583915.1"
                     /translation="MTGPIIVPERQARAEDQHPIAAAGQSFVIHEWSGSGPGYLHIHH
                     ADDEAWHVLEGTLTFRFNDKTIEAGAGTTVFVPAGVAHTYYEAAGPTRYLIIMTPRLR
                     ELIAALQQAPREKHNAIMRQFASEVVE"
     gene            1448..1795
                     /locus_tag="VGT44_23885"
     CDS             1448..1795
                     /locus_tag="VGT44_23885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007918901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEV2583916.1"
                     /translation="MTPIYPTDRESLERDCDSEFFIASGPGGQHRNKVETGVRLVHRP
                     TGMTVTATERRSQRANREAAFERMQARLEALQHVDIPRKPTRPTAASRVRRVNEKRVS
                     GMRKKLRSTPAEE"
     gene            complement(1858..3603)
                     /locus_tag="VGT44_23890"
     CDS             complement(1858..3603)
                     /locus_tag="VGT44_23890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583917.1"
                     /translation="MPTDGIFSTSGASRVALLATSNGPNTTAIVADANGPSSTAIQAT
                     SVDPSSTAIQATSGSASSAGAPPTASGACIHAISNDNLPCILAENTNDFNAITAKGLP
                     ALDAFDNPPPPSVITIPPGAVPGDGSITVLPPVEGFIAVIQAHASVDLAAGIIATSQN
                     GPGAVVSSAGATGLTAAAGLSPDSHLMGAGHAIVATTNVQNAAGLFASSDSGPGIEAS
                     SPDNAAIQATSQSGAGVTATSTSGAGIVVASTSGVGARISSQQATGISVAVGLPVDVG
                     LEQRFLELPGHAIAAVTEASGAFGLLAQSNKGPGARISSQQATGMVVFVGPEQANVES
                     AGHAIAASTNAQNGAGLFVSSANGPGVEASSLNNSAIKAASESSFGIEATSGGATALL
                     ARSSKGPGAVVSSDVATAIIASVGFGSSLSSNLSSEEAALESVGHAIAATTNKINAAG
                     LFAKSESGAGVEAVSNTGPGILVASKSNIAVDARSGSGTAVRARCLGGVGLSVEGLLE
                     VSEPAIGAITAHGGETSLQVQNPAATTKSTILLTPLGNPGAFLWINTRAAGSFTIGAS
                     QPLPGGLVIQFLIIN"
BASE COUNT          841 a         1153 c         1158 g          776 t
ORIGIN      
        1 gcgcacgatg ccgcccaaag agcgaggagc tgatgatccg aatcggtgag ggttccacca
       61 cggcggatcg tcacgaaacg agggtcgcgg tctttcggga ggatcatacc cagcacccct
      121 tctgctcgca tctgcccaac aggtaaatag ctggctcatc tatcagcata cgccctgacg
      181 aaaaccttcc tcaatcgagg tcatgtgctc agccgcatgc cccgctctcc cggcaaacaa
      241 gtcgccggcg gtcgcgtcac cgacgacggt gggcaccgga gcggccagct gatcatcggt
      301 gaactggcgc aggaggtcca cggtgtcctg ccgcgccgcc aggtaatccg ctgccagcat
      361 ctccagactg tcgtcacggt gcgcgtccac ataggcttga ttgacgctgg cgacagccat
      421 ccccagctgc tcctcctcct ccggggtcag cgcgcttaga ttgcccagga tgcccggcat
      481 cggcattccc tccgggtatt gaagacgtaa tagaacgtct cgagtctcgc cggcgagtgt
      541 acagcgcagc atttgctgga tgttgcgctc gtttttcacc aggtgagcca aatgatcctt
      601 ggcgcgccac ggagctgctc cgggaacttc actcgccgtt gccgggcgct ccaggtcttc
      661 cggactcagc ccttgaaaaa acgccatgac ctgcgtctgc gatgccgcaa gtgtgtccaa
      721 aatctctgcg cgagttggca tgatgaccct cctggaaacc agcgggaagc accaggcgtg
      781 tccagacgac tccgccgcgg taattgtaca gattccacca gtttaaagtt tgtatgtaca
      841 ccattcccat tacacgaggg aaatctggaa atgtcaaaaa caaagtcttg caaaggatca
      901 ggcgaggtta accagtttga actgtacaac gacatgatgt atgttatggg gtgatcccct
      961 tatatatggt ataaagaggg cagtcactcg tgtgagagga ggaattctga tatcatgacc
     1021 ggacccatta tcgtcccgga gaggcaggcg cgagcagagg accagcatcc catcgcagca
     1081 gccgggcagt cctttgttat tcacgaatgg agcggaagcg gcccaggcta tctacacatc
     1141 caccatgctg acgatgaggc atggcacgtt ctggagggaa ccctgacatt ccgtttcaac
     1201 gacaaaacca tcgaggccgg cgccgggaca accgtgtttg tgccagcagg agttgcccac
     1261 acatattatg aggcggcagg tcctacgcgg tacctcatca tcatgacacc gcgcctgcgc
     1321 gagcttattg ccgctttgca gcaagctcct cgcgaaaagc ataacgcgat catgcgccag
     1381 tttgcgtcag aggtggtgga gtaggcgatg aagttagaca ctgacttatt gaacaagagg
     1441 agcttttttg acgcccatct atccaactga ccgggaatcg cttgaacgcg attgcgatag
     1501 tgagttcttc atcgccagtg ggcctggggg acaacatcgc aacaaagtcg agacaggcgt
     1561 gcggctggtg catcgcccga ccggtatgac cgtcactgcc accgagcgcc ggtcgcagcg
     1621 cgccaaccgc gaagccgcct tcgagcgcat gcaggcgcgc ctggaagcat tgcagcacgt
     1681 tgacattcct cgcaaaccaa cgcgccccac ggcagccagc cgcgtacgcc gcgtcaacga
     1741 gaagcgcgtc tctggaatgc gcaaaaagct gcgctcaact ccggcggaag aatagagcaa
     1801 gtcagcttcg acccactgct ggacacgtaa ggatgatgtg acagggttat atggcgctta
     1861 gttgatgatc aagaattgga taaccagccc tcccggcagc ggttgactcg cgccaatcgt
     1921 gaagctgcct gctgcgcgcg tgttgatcca gagaaaggcc cctggattgc caagtggggt
     1981 cagtaaaatg gtgcttttgg tggtagctgc tggattttgg acctgcaatg aagtttcacc
     2041 tccgtgtgca gtgatcgctc cgatggcagg ttctgacacc tcgaggagtc cttccacgct
     2101 cagcccgaca ccgccgagac agcgtgcgcg cacggcggtg ccactcccgc tcctggcatc
     2161 cacggcaata ttgctcttgg atgccaccag gatgcccggg ccggtattac tcaccgcctc
     2221 gacaccagcg ccactctcac ttttggcgaa gaggccggcg gcattgatct tattggtagt
     2281 tgccgcgata gcatgaccga ccgactccag tgctgcttct tccgaactaa gattggaact
     2341 aagactggag ccgaagccga cggacgcgat gatagcggtc gcaacgtcgc tggagaccac
     2401 ggcgccaggc cctttgctgc tcctggccag cagggcagtc gcacctccgc tggtagcttc
     2461 aatcccgaaa ctgctctcac tggcggcctt gatagcagaa ttattcaggc tggacgcctc
     2521 gacaccagga ccatttgcac tgctgacgaa aaggccggct ccgttttggg cattcgtgct
     2581 ggcagcgatg gcatgaccgg ccgactccac gtttgcctgc tcaggaccga cgaaaacaac
     2641 catgccggtc gcctgctggc tggagatcct ggcgccagga cctttgttac tttgagccag
     2701 gaggccgaat gcccctgacg cctcggtaac agcagcgatg gcgtggccag gcagctccag
     2761 gaatcgctgc tccagaccaa catcgaccgg aaggccgaca gcaacggaga tgccggtcgc
     2821 ctgctggctg gaaatcctgg cgccgacgcc actcgtactc gccacaacga tacccgcacc
     2881 gctcgtactc gttgcagtga cacccgcacc gctctggctg gtggcctgga tcgctgcatt
     2941 atccgggctg gatgcctcga tcccggggcc actatcgctg ctggcgaaga ggccggccgc
     3001 gttttgcacg ttggtggtag ccacgatggc atggccagca cccatcagat gactgtcagg
     3061 ggaaaggccg gcggcagcgg tgaggccggt cgcccccgcg ctggagacga cggcgcccgg
     3121 cccattctgg ctcgtggcta ttattcccgc cgccaggtcg acagatgcat gtgcctggat
     3181 gacggctatg aaaccctcaa ctggagggag gacggtgata ctaccgtcac cagggacagc
     3241 accgggggga atggtaatca cacttggagg tgggggatta tcaaacgcat cgagagcggg
     3301 aagccctttg gcggtgatag cgttgaaatc attcgtgttc tccgccagga tgcaggggag
     3361 attatcgttg ctgattgcat ggatacaggc gccagacgct gtcggaggag cgcccgcgga
     3421 ggacgcgcta ccgctcgtag cctggatagc agtactgctc gggtcgacgc tcgtggcctg
     3481 gatagcggta ctgctcgggc catttgcatc ggccacgatt gcggtagtgt tcgggccatt
     3541 acttgtggcc aggagagcaa ctctactagc gcccgatgtc gaaaaaatac cgtcggtagg
     3601 cataatatcc ctcctatgga ctagcacaac tactggggat tttatcatat ccactcccta
     3661 ccttgcccaa agctctataa tacgctatac tcgttttcca gaaacacatc agtgattgag
     3721 aagatgaaca tatctcgtga ggctataggg ggagagtcaa ttcatgaagc ccgaagaccg
     3781 cgcgcgccag aatatcgaca ggatgctcga agaggctggc tgggtcgtac aggaccgcag
     3841 ctacttcgac ctgtccgccg ggcggggggt ggctattcgc gaactctcga tggcaacggg
     3901 cgcggcggat tatgcgctgt ttgtggat
//
LOCUS       DASZAK010001364         1579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_354275, whole genome shotgun sequence.
ACCESSION   DASZAK010001364 DASZAK010000000
VERSION     DASZAK010001364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1579
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354275"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VGT44_23895"
     CDS             <1..189
                     /locus_tag="VGT44_23895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEV2583918.1"
                     /translation="LPVTVGKGQVIKGWDEGLVGMKAGGIRRLYIPASLGYGAAGSSP
                     AIPPNAALIFDVQVLTVK"
     gene            complement(193..600)
                     /locus_tag="VGT44_23900"
     CDS             complement(193..600)
                     /locus_tag="VGT44_23900"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007910124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEV2583919.1"
                     /translation="MKQRVLFLCTHNSARSQMAEGLLRSLGSMGYEAFSAGTEATHVR
                     PLAIKAMAEVGIDISGQSSKTLERYLHEPFDEVITVCDTAAETCPVFPGAQHRRHWSL
                     PDPSKATGSEEEQLAVYRRVRDALRARIEAELL"
     gene            complement(597..1379)
                     /locus_tag="VGT44_23905"
     CDS             complement(597..1379)
                     /locus_tag="VGT44_23905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007911568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEV2583920.1"
                     /translation="MDATIATGQSPAFLKLLAHDLRWQLLTALAHSDYSVQELVQRID
                     EPQNLVSYHLRRMREQEVIAERRSSADRRDVYYSLDLARFQERYADALGSLHPALDHA
                     EATQTADAALLEHKAARVLFLCTANSARSQMAEALTRHLSQGRVEAFSAGSHPTHLHP
                     YAVQVMASIGIDISQQRSKHFDEFLGQSFDRIITVCDRVRESCPNFPGDPERIHWSIA
                     DPVAASGSEQEVFHAFEQVSLQLTNRIRFLLILLEREQRGTL"
BASE COUNT          317 a          465 c          460 g          337 t
ORIGIN      
        1 ttgccggtga cggttggcaa ggggcaggtc atcaagggct gggatgaggg actggtgggt
       61 atgaaggccg gtggcattcg ccgcctgtac attccggcca gcctgggata cggcgcagcg
      121 ggcagctcgc ctgccattcc acccaatgcg gcgctcatct tcgatgtaca ggtgctgacc
      181 gtcaagtaat cgttacagca gttctgcttc gatgcgcgcc cgtagcgcat cgcgcacgcg
      241 gcgatacacc gccaactgtt cctcctcgct gcccgtcgcc tttgatgggt cgggcagcga
      301 ccagtgacgg cggtgttgcg cgccgggaaa gaccgggcag gtctccgcgg cggtgtcgca
      361 caccgtaatc acctcgtcga atggctcatg caagtagcgt tccagcgtct tcgatgattg
      421 tcctgagata tcaatgccca cttcggccat cgccttgatc gccaggggcc gcacgtgcgt
      481 cgcctccgtc cccgcgctga acgcctcata acccatgctg cccagggatc gcagcagccc
      541 ctcagccatc tgcgaacgcg ctgaattgtg cgtgcagaga aacagcaccc gttgtttcat
      601 aatgttcccc tttgctcgcg ctccaacaat atgagcaaga aacgaattcg gttcgtgagt
      661 tgcagcgaga cttgctcaaa tgcatgaaat acctcttgct ctgaaccgct cgcggcaaca
      721 ggatcggcaa tgctccaatg gatgcgctca ggatcgcctg ggaaatttgg acatgactct
      781 cgcacgcgat cgcaaaccgt gatgatacgg tcaaaagatt gaccgaggaa ctcatcaaaa
      841 tgctttgatc gctgctggct gatatcgatg ccaatcgatg ccatcacctg taccgcgtag
      901 ggatgcaaat gggtaggatg gctacccgcg ctgaacgcct cgacgcgccc ctggctcaga
      961 tgcctcgtga gcgcttctgc catctgcgaa cgcgcgctat ttgccgtgca gagaaacaag
     1021 acgcgcgccg ctttgtgttc gagcagcgcc gcgtcggcgg tctgagtcgc ctcagcatgg
     1081 tccagcgctg gatggagcga accgagggcg tcggcatacc gctcctggaa gcgggccagg
     1141 tcaaggctgt agtacacgtc gcgcctatcg gcagaactgc gccgttcggc aatcacttcc
     1201 tgctcgcgca tccgccgcag gtgatacgac accaggttct gtggctcatc gatgcgctgc
     1261 actaactctt gcacgctata gtcgctgtgc gccagggctg tcagtagttg ccagcgcagg
     1321 tcatgcgcca gaagcttgag aaacgcgggc gattggccgg ttgctatggt cgcatccata
     1381 aattcctcca attgtatttg tatcagtatt atttgatata atgatactat catatttgag
     1441 gaaaatcaag cgatgggcta tctgcgtaga caagtggcgt ccaggctgtc atcctgagcg
     1501 cagcgaagga tctgcctcgc cgggcgctga gatccttcgc tgcgctcagg atgacagact
     1561 ggacgccact tgtctacgc
//
LOCUS       DASZAK010001365         5813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_211474, whole genome shotgun sequence.
ACCESSION   DASZAK010001365 DASZAK010000000
VERSION     DASZAK010001365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211474"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VGT44_23910"
     CDS             complement(<1..236)
                     /locus_tag="VGT44_23910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV2583921.1"
                     /translation="MFYDHTKIFVKAGNGGNGSMHFRREKFAPFGGPDGGDGGRGGSV
                     YIEATPNANTLIEYRYRQHFKAGSGEAGARQKMHG"
     gene            complement(307..606)
                     /locus_tag="VGT44_23915"
     CDS             complement(307..606)
                     /locus_tag="VGT44_23915"
                     /inference="COORDINATES: protein motif:HMM:NF023857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family antitoxin"
                     /protein_id="HEV2583922.1"
                     /translation="MTQKDRAQSKKSRSRIPTFKTIEEEAAFWDTHSSEEFADELIPV
                     EDVAFVKTRSKKALTVRFDEDTLEELAREAREKGIGPSTLARMVIVEHLRIRRAS"
     gene            complement(603..884)
                     /locus_tag="VGT44_23920"
     CDS             complement(603..884)
                     /locus_tag="VGT44_23920"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEV2583923.1"
                     /translation="MKIYELVVEPGREEHIARHQVSVEEVEEVIFDAPFIRKARQGRY
                     HIIGQTESGRYLTVFVAPREKGVYGLVTARDADDAERKAYLRHSKQKRQ"
     gene            complement(954..2582)
                     /locus_tag="VGT44_23925"
     CDS             complement(954..2582)
                     /locus_tag="VGT44_23925"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEV2583924.1"
                     /translation="MQNIRETLPIGSVVDGRYQIEQLLGRGGFGAVYLVSDQQARQNL
                     FALKEVIDTDKRERARFTFEAELLKRIDHPALPHVYHVFEDDVHDRAYMLMNYVEGPN
                     LEKLRAQQPGKQFSVVQVLTIMGPIMDAVSYLHERHPPIIHRDIKPSNIIVPNSGEGT
                     VLVDFGIAKFFDPDATTTAIRHASPGYGAPEQYATGTNTRTDIYGLGATMYALLTGIV
                     PKDSFFRITQFSSRGSDPLEPIQRFAPGVPYHVSNAIYRAMAIDIENRFPTVQEFKNA
                     LNSLPPMQHQSPLMATPLVEAGLAGTAFRDGPPPTSRNDSMVAMPGRRDDSRRKANRG
                     LLLLLTLLVVLLLGVAAADLLLPAFLNHHPGPSTPAPAATVQHKPTATVAPHPTVTPG
                     LSPTTASPPSASDPTPSPTPSLTPSPTPSVTPSPTPTLGTTYHGSIHNTPANSSATMS
                     LTSIKQNGGAFQGNFNVNLPLSGSGPFTGTVNNARSVQFTVHSSQVPAPLFFQGNLNS
                     DGSLSGTYCSLDQTGHCNPAAGGYGTWQVSPASF"
     gene            complement(2665..4401)
                     /locus_tag="VGT44_23930"
     CDS             complement(2665..4401)
                     /locus_tag="VGT44_23930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2583925.1"
                     /translation="MQYVRETLPVGSMIEDRYQIKQLLGRGGFGAVYLVNDQQANQQL
                     FAHTEVIDTDKLQRDHFAFEAELLKRVTHPALPRVYQVFEDDEHDRAYMLMNYIEGPN
                     LEKLRSQQPDKQFSVEQVLAIMGPIMDAVSYLHEQQPPIIHRDIKPSNIIVPISDEGA
                     TLVDFGIAKIMDPEGTTTAVRHASPGYCAPEQYGTGTNIRTDIYCLGATMYTLLTGIV
                     PKDSFFRITQLGSRGNDPLEPIQRFAPDVPSSISNAIYRAMAMNIDKRFATVEDFRRA
                     LHSFQFMRRSPIFLAKPLASTKQSCAAFRESLPTTGGVAPRFVLPVRSEKSTPRRIGS
                     GLLLLLCLSLVLLLSLAAADLVLPAFLSRHQGPITPAQAAAVQHTPAATVTVTHQPTV
                     TPGVSPTTANPPSAATQSPSSTPTSSTPSPLPSPTPSSTPSPTPSPSPTPTTIPSLAG
                     KYTGTINDTPASISANMTLAAINQNGGAIQGKFKVGAPLIGNGPFTGTVNNVGIIQFT
                     VTSTQITARLWFRGTLHSDGSISGTYCSLDQTGHCNPAAGGSGTWQVAPASQGSIVVG
                     AWRLIRSLPSVL"
     gene            complement(4554..>5813)
                     /locus_tag="VGT44_23935"
     CDS             complement(4554..>5813)
                     /locus_tag="VGT44_23935"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV2583926.1"
                     /translation="RIVIVGAGFGGLQAALALHPAPARVTVIDRQNHHLFQPLLYWVA
                     TAGLSPADITSPIRGILKRQKNTEVVMAEVTGVDVEKRRVLMGEHSVPFDYLVLATGA
                     RDNYFGHPEWEQDAPGLKSIVEATAIRRKILLAFEAAEVESDPQKIKELLTFVLVGAG
                     PTGVEMAGAIAELAHKALSSDFRHIDTKQARIVLVEAAPRILAAFPESLARKTERKLR
                     RMGVEVHIGQAVTQIDEHGVTLDSQHIAAHTVIWSAGVSASPAGAWLGAEVDRAGRVT
                     VAPDLSVPDHPDIFVIGDTASVMQEGKPLPGVAPVAMQAGRYVASVIKHRLTGEQAPV
                     PFHYTNKGNLATVGRSYAIVEIGKLRLTGLIAWVSWLLIHIYYLIGFRNRIVALFQWA
                     WTYFTYSRGARLITFEKPGQEAAKMRE"
BASE COUNT         1219 a         1539 c         1681 g         1374 t
ORIGIN      
        1 ccgtgcatct tctgccgcgc gcccgcctcg cccgaacccg ccttgaagtg ctggcgatag
       61 cgatattcaa tcagcgtatt ggcgttcggt gtggcctcga tatagacgct gccccctctg
      121 ccgccatcgc cgccatctgg cccgccaaat ggcgcgaact tctcccggcg aaagtgcatg
      181 gaaccattgc cgccattccc cgccttcaca aatattttcg tatggtcgta aaacatagtt
      241 tatccctggt ctgccttttc ttctcggaaa tctcaaagtg ctttgtaatg tggaacacag
      301 cggtaatcag gaagctcttc ttatacgaag atgctctaca atcaccattc gagcaagagt
      361 tgaagggccg atacctttct ctctcgcttc tctggcgagt tcctcaagtg tgtcttcgtc
      421 aaaccggact gtaagagctt tcttggagcg agtttttacg aacgccacat cctcgacagg
      481 aatgagctcg tctgcgaatt cttcgctcga gtgggtatcc cagaatgcag cctcttcttc
      541 aattgttttg aaggttggta tgcgacttct ggatttcttc gattgtgctc gatctttttg
      601 tgtcattggc gtttttgttt actgtgtcgc aggtatgctt tacgctctgc atcatctgcg
      661 tctctagctg tgacgaggcc gtacactccc ttttctcttg gagcaacaaa cactgtcagg
      721 tatctgccag attcggtttg tccaatgatg tgatagcgtc cctgccttgc ctttcgtata
      781 aaaggcgcat caaagatgac ttcttcaact tcttcgacac ttacctgatg acgtgcaata
      841 tgttcttcac gtccaggctc tacaacaagc tcatagattt tcacgggctg cacatctttt
      901 tgcagctatt gtaatacgaa agatgtcggc tgtcaaatag aatgattctt acgtcagaat
      961 gaagcaggcg acacttgcca ggttccatag ccacccgcag cggggttaca atgccctgtc
     1021 tggtccaggc tgcagtaggt ccccgagagg ctgccatcac tgttgagatt gccctgaaaa
     1081 aagagcggtg ccggaacctg gctgctatgc accgtgaatt ggacagacct ggcattgttg
     1141 actgtgccgg taaatgggcc gctgcctgag aggggcagat tgacattgaa attaccctga
     1201 aaagcgcctc cattctgctt gatggaggta agagacatgg tcgcgctgct atttgccggg
     1261 gtattgtgta tcgacccgtg gtatgtcgtg ccaagcgtcg gcgtgggtga aggagtcact
     1321 gatggcgtcg gcgaaggagt gagtgatggc gtgggtgatg gagttgggtc agaagcggaa
     1381 ggagggctgg ccgttgtcgg gcttaaccct ggcgttaccg tcgggtgagg tgcgacggtg
     1441 gctgtgggtt tgtgttgtac cgtcgcagca ggcgcaggtg ttgacggccc tggatgatga
     1501 ttgaggaacg caggcagcag caaatcggct gcggcaacac cgagtaacag cactaccaac
     1561 aacgtaagga gcaggagcaa tcccctattg gcctttctac gtgaatcatc cctgcgtccc
     1621 ggcatagcaa ccattgagtc attcctgctc gttggtggtg ggccgtctcg aaaagcggtg
     1681 ccggccagcc ctgcttctac gagtggcgta gccattaatg gtgactggtg ctgcatggga
     1741 ggcagcgaat tgagcgcatt tttgaattcc tgaacagtgg gaaagcggtt ctcgatgtcg
     1801 atcgccattg cgcgatagat tgcatttgag acgtggtacg gaacgcctgg cgcgaagcgt
     1861 tggattggct cgagcgggtc gcttccccgg ctgctaaact gcgttatgcg gaagaaggaa
     1921 tcctttggca caatccctgt cagcagcgca tacatggttg cgcccaggcc atagatatcc
     1981 gtgcgcgtat tcgtcccggt agcgtactgt tctggggcgc cgtagccggg tgaagcgtgt
     2041 cgtatagcag tggttgtcgc gtccgggtca aaaaatttcg ctatgccaaa gtcgaccaga
     2101 accgttccct cgcccgaatt gggtacgata atattagacg gtttgatatc gcgatggata
     2161 attggcgggt gccgttcgtg caggtaggac acagcatcca taattggtcc cataatagtg
     2221 agtacttgca cgactgaaaa ttgcttcccc ggctgctgcg cgcgcagttt ttcgaggttt
     2281 ggcccctcaa cgtagttcat gagcatataa gcgcggtcgt gtacatcatc ttcgaacacg
     2341 tgatagacat ggggcagcgc aggatgatca attcgtttga gcagttctgc ttcaaacgta
     2401 aaacgagctc gctctcgctt atctgtatcg ataacttctt tgagggcaaa caaattttga
     2461 cgggcctgtt gatcgcttac caggtagact gcgccaaatc cccccctgcc gagcaattgc
     2521 tcgatctggt agcgcccatc aaccacgctc ccgatcggga gcgtctcgcg tatattttgc
     2581 aaaatatact ccggacgacg gcataacgtt gtacatggac gaataaaagc actttcttaa
     2641 atcacgaatg ccaggtaagt tgtgttacag aacagaaggc aaagagcgta ttagccgcca
     2701 cgctccaacg acgattgaac cttgtgaggc gggagctacc tgccaggttc cagagccgcc
     2761 tgcagcgggg ttacaatgtc cagtctgatc caggctgcaa taggtccccg aaatgctgcc
     2821 gtcgctgtga agcgtacccc gaaaccagag tcgcgctgtg atttgagtgc tggttactgt
     2881 aaattggata atccccacgt tattgactgt gccagtaaat gggccgtttc ctatgagggg
     2941 cgcaccgact ttgaatttcc cctggatggc tccgccattc tgattgatcg cggcgagagt
     3001 catgttcgct gagatgctgg ccggagtatc gttgatcgtt ccagtatact tgccggcgag
     3061 cgaaggtatg gtggttggag tcggcgatgg cgatggtgtc ggagatggcg ttgatgatgg
     3121 tgtcggagat ggcaatggtg atggcgttga tgacgttggt gtcgatgatg gtgactgtgt
     3181 agcagcggaa ggagggttag cggttgtcgg actcactccc ggcgtcactg tcggttgatg
     3241 ggtaacggtc acagtggcag caggagtgtg ttgcactgcc gctgcctgtg cgggtgtaat
     3301 cggaccttga tggcgactga ggaatgcagg cagcaccagg tcggcagcag caaggctgag
     3361 caacaggacg agcgacaggc aaaggagcag gagcaatccc gagccgatcc tgcgaggtgt
     3421 gctcttttcg ctgcgtactg gaagcacaaa gcgtggagca accccacccg ttgtgggcag
     3481 gctctcacga aaagcggcgc agctctgttt tgtgctggcg agtggcttag ctagaaatat
     3541 cggggagcgc cgcatgaatt gaaatgaatg gagtgcgcgc ctgaaatcct caactgtagc
     3601 aaagcgctta tcaatgttca tcgccatcgc gcgatagatt gcattcgaga tagatgaggg
     3661 aacgtctggt gcgaagcgtt ggattggctc gagtggatcg ttgccccggc tgccaagctg
     3721 tgtaatgcga aagaaggagt cttttggcac aatgcctgta agcaacgtat acatggttgc
     3781 gcccaggcag tagatatcgg tgcgtatgtt cgtcccggtg ccatactgtt cgggcgcgca
     3841 gtagcccggc gaagcatggc gaaccgccgt cgtcgtcccc tccgggtcca ttatcttcgc
     3901 gatgccaaaa tcaaccagcg tcgctccctc gtctgagatg ggtacgatga tgttcgacgg
     3961 tttaatgtcg cgatggatga tgggcggctg ttgttcgtgc aggtaagaca cggcatccat
     4021 gatcggcccc atgatggcga gaacctgctc gaccgaaaat tgtttgtccg gctgctgaga
     4081 gcgtagtttt tcgaggtttg gcccctctat atagttcatg agcatatagg cgcggtcgtg
     4141 ttcatcatcc tcgaatacct ggtagacacg aggcagcgcg ggatgggtaa ctcttttgag
     4201 caactccgcc tcaaaagcaa aatggtcccg ctgcagttta tccgtatcga taacttctgt
     4261 gtgggcaaac aattgctggt tggcctgctg gtcgtttacc aggtagactg cgccgaatcc
     4321 gcccctgccg agcagttgct taatctgata acggtcttca atcatgctcc cgaccgggag
     4381 cgtctcacgt acgtattgca aaatgcaccc ctgtcctgga ataaaacgat gtcacaaaat
     4441 gagtacactc taaaaacacg attatcgagc aggttgcgat acaccatgcc gggtatttaa
     4501 taatatataa cgtatcagga tagtggatgt ctcactgcta gatattctgg caatcactcg
     4561 cgcatcttgg cagcttcttg ccccggcttt tcaaaggtaa tcagtcttgc tccccgcgaa
     4621 taggtgaagt aagtccaggc ccattggaaa agcgctacga tgcgattgcg aaagccgatc
     4681 aggtagtaga tgtggatcag cagccacgac acccaggcga tcagccccgt caggcgcagc
     4741 ttgccgatct cgacgatggc atacgagcgc cctaccgtcg ccaggttgcc tttgttggtg
     4801 tagtggaatg gcacgggcgc ttgttcgccc gtcagtcgat gcttgataac cgaggcgaca
     4861 tatcgcccgg cctgcatcgc caccggcgcg actcccggca gcggcttgcc ctcctgcatg
     4921 acgctggccg tatcgccaat cacgaagatg tcggggtgat ccggcacgga gaggtcaggg
     4981 gcaactgtga cgcgaccggc ccggtcaacc tccgcgccaa gccatgcccc tgccggtgat
     5041 gccgagacgc ccgcgctcca gatgaccgtg tgcgccgcga tgtgctggct gtctagcgtc
     5101 acgccatgct cgtctatctg tgtgacagcc tggccgatat gcacctccac gcccatgcgc
     5161 ctcagcttgc gctcggtctt gcgcgccagc gattccggaa aggccgccag gatgcgcggc
     5221 gcggcctcga ccaggacgat gcgcgcctgc ttcgtatcaa tgtggcgaaa gtcggatgac
     5281 agcgctttgt gcgccagttc cgcgatagct ccggccatct cgacgcctgt tggccccgcg
     5341 ccgaccagca caaacgtcag tagttccttg atcttctgcg ggtcgctttc gacctccgcc
     5401 gcttcgaagg ccagcaggat tttccgccgg atggcggtgg cctcgacaat cgatttcaga
     5461 cccggtgcgt cctgctccca ttcgggatgc ccaaagtagt tatcgcgcgc gcccgtcgcc
     5521 agcaccaggt agtcaaacgg cactgaatgc tcgcccatca gcacgcgtcg tttctctacg
     5581 tccacgccgg tcacttcagc cataacaacc tcggtattct tctgccgctt gagaatgccg
     5641 cgaataggcg aggtgatgtc agcaggcgag agaccagccg tcgcgaccca gtagagcagc
     5701 ggctggaaca ggtgatggtt ttgccggtcg ataacggtaa cacgcgccgg ggcagggtgt
     5761 agcgccagcg ccgcttgcaa gccgccgaag cccgccccga cgataacgat gcg
//
LOCUS       DASZAK010001366         1808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_567381, whole genome shotgun sequence.
ACCESSION   DASZAK010001366 DASZAK010000000
VERSION     DASZAK010001366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567381"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /gene="trpE"
                     /locus_tag="VGT44_23940"
     CDS             <1..1396
                     /gene="trpE"
                     /locus_tag="VGT44_23940"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV2583927.1"
                     /translation="RIARYSFIGIDPYLVMTQRGDVATLRHIRQQDERIHTTTIEQIT
                     FHDPLDFIQAELGQYRLIRPESMAHDRLPSFYGGAVGYLSYETVAHFERLPVPEQDEL
                     GLPLAMFCFTETVLVFDHLKRRVRIVTHLHLDAPDLEAEYLRVFNLIEHVRERLRQEP
                     RLPDEPTPIGDHHAAQVSSNRAPAEFEAMVEQAQEYIRAGDIFQVVLSQRLSRHVDAA
                     PFTVYRALRAINPSPYMFFLDLEDFHIIGASPELLVRVEDGEVTIHPIAGTRRRGNDP
                     QSDALLAEELLNDPKERAEHVMLVDLGRNDVGRVAIPGSVEVTQLMEIERYSHVMHLV
                     SNVTGRLRPDVTPYDGLRAGFPAGTVSGAPKIRAMEIISELEGTQRGVYAGAVGYFSH
                     SGNLDTAIALRTMLMRDGQAYIQAGGGIVADSVPSEEYEESMNQARALLRALDEAEAM
                     AEERRGGIYAALNR"
     gene            1377..>1808
                     /locus_tag="VGT44_23945"
     CDS             1377..>1808
                     /locus_tag="VGT44_23945"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007905682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV2583928.1"
                     /translation="MLLLIDNYDSFTYNLYQYLSELGEEVVVRRNDMVTLEEIAVMHP
                     DQIVISPGPGTPDEAGISGRVIEAFGPRIPTLGVCLGHQAIGQVYGGRVIRAPQPMHG
                     KTSRIYHKGQGVFRGLSSPLEANRYHSLIVERATLPADLEIT"
BASE COUNT          353 a          585 c          555 g          315 t
ORIGIN      
        1 acgcatcgcg cgctactcgt ttatcggcat cgatccctac ctggtcatga cgcagcgcgg
       61 cgacgtagcc acgctgcgac acattcgcca gcaggacgag cgcatccaca cgaccaccat
      121 cgagcagatc acctttcacg atccgctcga tttcatccag gcggaactgg ggcagtaccg
      181 gctgatccgg cctgagagca tggcgcatga caggctgccg agcttttacg gcggggccgt
      241 cggctacctg tcctacgaaa cggtggcgca cttcgagcgc ctgccggttc ctgagcagga
      301 cgagctgggg ctgccgctcg ccatgttctg cttcaccgaa acggtgctgg tcttcgatca
      361 tctcaagcgc cgcgtacgca tcgtgacgca cctgcacctg gacgcgcccg acctggaagc
      421 cgagtacctg cgcgttttca acctgattga acacgtcagg gagcgcctgc gccaggagcc
      481 gcgcctgccg gacgaaccca cgcccatagg cgaccaccac gccgcgcagg tctcttccaa
      541 ccgcgctccg gcggagttcg aggcgatggt tgagcaggcg caggaataca tccgcgcagg
      601 cgacatcttc caggtcgtgc tttcgcagcg cctgagccgc catgttgacg ccgcgccatt
      661 taccgtctac cgcgccctgc gcgccatcaa cccatcgccc tacatgttct ttttagacct
      721 ggaagacttc cacatcatcg gcgcatcgcc cgaactgctg gtgcgcgtcg aagatggtga
      781 agtgaccatc catcccatcg ccggaacgcg caggcgcggc aacgacccgc agagtgacgc
      841 gctgctggcg gaggagctgc tcaacgaccc gaaggagcgc gccgaacatg ttatgctggt
      901 tgacctgggg cgcaacgatg tcgggcgcgt cgcgatcccc ggcagcgtag aggtcacgca
      961 actcatggaa atcgaacgct attcgcacgt catgcacctg gtctcgaacg tgaccggcag
     1021 gctgcggccc gatgtgacgc cctacgacgg gctgcgcgcc ggtttcccgg caggcacggt
     1081 ctccggcgct cccaagattc gcgccatgga gatcatcagc gagcttgagg gaacgcagcg
     1141 aggcgtctac gccggggcgg tgggctactt cagccatagc ggcaacctgg acaccgccat
     1201 cgctctgcgc accatgctga tgcgcgacgg ccaggcgtac atccaggcgg gcggcggcat
     1261 cgtagccgat tctgtaccat ccgaggaata cgaggaaagc atgaaccagg ccagagcgtt
     1321 gctgcgcgcc ctggacgagg cggaggcaat ggcagaagaa cgcagaggag gcatctatgc
     1381 tgctcttaat cgataattac gacagtttca cctacaacct ctaccagtat ttatcggaac
     1441 tgggcgagga ggttgtggtg cggcgcaacg atatggtgac gctggaggag atcgcggtaa
     1501 tgcatcccga ccagatcgtg atctcgcccg gccctggcac accggatgaa gcgggtatct
     1561 caggcagggt gatcgaagcc ttcggcccgc gcatcccgac actaggcgtg tgcctgggac
     1621 accaggccat tgggcaggtt tacggtgggc gtgtgatacg cgcgccacag ccgatgcacg
     1681 gcaagacctc gcgcatctat cacaaggggc agggcgtctt tcgcggattg tcctcgccac
     1741 tggaagccaa tcgctatcat agcctcattg tcgagcgcgc cactctcccg gctgacctgg
     1801 agattacc
//
LOCUS       DASZAK010001367         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_354531, whole genome shotgun sequence.
ACCESSION   DASZAK010001367 DASZAK010000000
VERSION     DASZAK010001367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354531"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="VGT44_23950"
     CDS             <1..635
                     /locus_tag="VGT44_23950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEV2583929.1"
                     /translation="WGVWMIPLIGIPCALAAAVLFAARLFPRLHFDRRVLVSTVGVAL
                     LALMLTSAVWSAIPVLQGQAADLPVAGPTGGTSFGAGGGSSNISVNTGLVSYLEAHKG
                     SAKYLVAVASANEADSIILETNQPVMALGGFTGSDPILTTTQLAALVKSGEVRYFLVG
                     GNGGGAGGSQSALITWIKAHGTVVSSSQYGGSSSGGFGGSETLYEVTSAS"
     gene            1047..2390
                     /locus_tag="VGT44_23955"
     CDS             1047..2390
                     /locus_tag="VGT44_23955"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HEV2583930.1"
                     /translation="MIANKRLPISALHLLARCAIVLLLLLAFALRISLFHIVTSDYTV
                     FLSQWYDFIQTHGGFTAFKYSFSNYNVPYLYLLAIATYLPIPKLIAIKTISVLFDGVL
                     GLFAYLILSLKFRRSYAAAIGVLVILFAPTIVINGSAWGQADAVYAAFCLGSLYFLLK
                     ERPAWACLFFGLAISFKLQAIFFLPILLVLALRRKLPLRYLALIPAVFLLMLLPAFIA
                     GRDAWSLLTIYFGQITGTGTGIVGQFTNRPFNGGGNGAFGSGRFNGGGNFGGGFSSSS
                     LTLNAPSIYQWFPANAPAAWQWLGITLAALVVVAVGVMLLVSKKQITSSIILKVALVF
                     AIAIPFLLPEMHERYFYLADLISIIYAFYVPRRWFIAVIMQLCSLFSYMPYLINTQII
                     SLGLLAFGVLAIAIVAFVDLVMELLPGIQRWRKLSLFVDEQVPASQNDGLIEANK"
     gene            2478..>2871
                     /locus_tag="VGT44_23960"
     CDS             2478..>2871
                     /locus_tag="VGT44_23960"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEV2583931.1"
                     /translation="MRVALFITCFNDTLFPNVGIATTRLLERLGYAVDFPEAQTCCGQ
                     MHYNTGYQREAIPLVRHFVEVFAPAEVIVAPSASCAGMVRDLYPKAAALSGDKALIAA
                     VEQLVPRVYELSEFLVKREGITGVGAYFP"
BASE COUNT          539 a          869 c          768 g          695 t
ORIGIN      
        1 cctggggcgt gtggatgatc ccgctcatcg gcatcccgtg cgcgctcgcg gcagctgtgc
       61 tgttcgcggc tcgcctgttc ccccgcttgc acttcgaccg gcgcgtgctg gtctcgacgg
      121 tcggtgtcgc gctgctggcg ctgatgttga cctcggcggt ctggtctgcc atccccgtgt
      181 tgcaaggcca ggcagctgac ctgccggtcg ccgggccgac tggcgggaca agctttgggg
      241 caggtggtgg cagtagtaac atctccgtca acaccggact ggtgagctac ctggaagcgc
      301 acaaaggcag cgcgaagtac ctggtcgccg tcgccagcgc gaacgaagct gactccatta
      361 tcctggagac caaccagccc gtcatggcgc tgggcggctt caccggcagc gacccgatcc
      421 tgacgacgac ccagcttgcc gcgctggtca agagcgggga agtgcgctac ttcctcgtcg
      481 gcggcaatgg cggcggcgca ggcggtagcc agagtgcctt gattacctgg atcaaagcgc
      541 acggcacggt cgtttcgtcc agccaatacg gcggtagctc ttccggcggg tttggcggtt
      601 ctgaaacgtt gtacgaggtc acatcggcct cctgatgaac atgcaggaat gggataggaa
      661 tatgattatt cccaaacacg tccaataatg tcaccctgag cgaagcgaag ggtctcggtt
      721 gaggtgccgc cagatccttc gcttcgctca ggatgacagg gcagacatgg gttgtgaaac
      781 ttcattttcc tatcacattc caatctcttt cacatcttcc taacctatac tggaatagag
      841 ctaaaactct attggttgat tggctacaaa catttcatga tgaattttcc caatcaaagt
      901 cgggaatgtc atgctgagcg cagcgaagca tctcaccgcc cgccgacaga gacccttcgc
      961 ttcgctcagg gtgacaggcg aggaccgaac ctgatcgtaa aacttcatta caaaaattgt
     1021 aggaagacga ggttcatcga aaagctatga ttgccaataa aaggcttccc atatccgcgt
     1081 tacatctgct cgcgcgctgc gccatcgtgt tgttattgtt gctcgcattt gcactgcgca
     1141 tctcactatt tcatatagtc acaagcgact atacggtatt cctgagccag tggtacgact
     1201 tcatacagac tcacggtggt ttcactgcct tcaaatacag cttttccaac tataatgtgc
     1261 catatctcta cctgcttgcc attgccacgt acctgcctat tccaaagttg atcgccatca
     1321 aaaccatatc ggtcttgttc gatggtgtgc tgggtctgtt cgcctatctc attctcagtt
     1381 tgaagttcag gcgctcgtat gccgccgcca tcggtgtgct ggtgatcctg tttgctccca
     1441 cgattgtcat caatggctcc gcctgggggc aggccgacgc cgtctatgcc gctttttgtc
     1501 ttggcagcct ctacttcttg ctcaaagaac gccccgcctg ggcgtgtctc ttcttcgggc
     1561 tggccatttc attcaaactg caggccatct tcttcttgcc catcctgctc gtactcgcgc
     1621 tcaggcgcaa gctgccattg agatacctcg cgctgatccc ggcggtcttc ctgctcatgc
     1681 tgctccccgc cttcatcgcc ggtcgcgatg cgtggagcct gctgaccatt tacttcgggc
     1741 agatcaccgg cacgggcact gggatcgtcg gccaattcac caacaggccg ttcaacggcg
     1801 gaggtaatgg cgcgtttggc agtggtcgat tcaacggcgg aggaaacttc ggcggcggct
     1861 tctcgtcctc gtcgctgacg ctgaacgcgc cttcgatcta ccagtggttc ccggcaaacg
     1921 cgcctgccgc ctggcaatgg ctcggcatca ccctggccgc gctggtcgtc gtggcagtcg
     1981 gcgtcatgct gcttgtgagc aaaaagcaga ttacatcctc cattatcctc aaagtcgcgc
     2041 tggtgttcgc catcgccatc ccctttctgc tgccagagat gcacgagcgc tacttttacc
     2101 tggccgactt gatctcgatc atctacgcct tctacgtccc gcgcaggtgg tttatcgccg
     2161 taattatgca gctttgctca ctcttcagtt acatgcccta cctcatcaat acgcagatca
     2221 tcagtcttgg cctgctcgct ttcggcgttc tcgccatcgc cattgtcgct tttgtcgacc
     2281 ttgtgatgga gctgttacct ggcatccaac gatggagaaa gttatcgctc ttcgttgacg
     2341 agcaggtgcc tgcatcgcaa aatgacgggc ttatcgaagc aaacaagtag cgctaccttg
     2401 tgcgacatca gcgcgcttac aggtgctata atcaatcagg cacacattct ctgtaagttc
     2461 gttgaaagga acagccggtg cgagtcgcgc ttttcatcac gtgtttcaac gataccctct
     2521 ttcccaacgt cggcatcgcg accacgcggc tgctggagcg gctgggctat gccgtcgatt
     2581 tcccggaggc gcagacgtgc tgcgggcaga tgcattataa caccggctac cagcgcgagg
     2641 ctattccgct tgtacgccac tttgttgagg tcttcgcccc cgccgaagtg atcgtcgcgc
     2701 catcggcttc ttgcgccggc atggtgcgcg atctctatcc taaagccgcc gcgctctccg
     2761 gcgacaaagc gcttatcgca gcggtcgaac agctcgtccc gcgtgtctac gaactctccg
     2821 aatttctcgt caagcgggaa ggcatcaccg gcgtgggcgc gtacttcccg c
//
LOCUS       DASZAK010001368        10266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_70268, whole genome shotgun sequence.
ACCESSION   DASZAK010001368 DASZAK010000000
VERSION     DASZAK010001368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 10266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10266
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70268"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..629)
                     /locus_tag="VGT44_23965"
     CDS             complement(96..629)
                     /locus_tag="VGT44_23965"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEV2583932.1"
                     /translation="MTTTVLVAYATKYGSTREVAEAIAATLSLEGLEVEIQPAREVRS
                     LDGYGAVVLGAALYMGRWHKDARRFLKRHRAALSQRQVAIFALGPLSAAEEDRRGSRE
                     QLDRALAQVPWLTPVEVEMFGGMINPAQLHFPFNHMPAGDARDWKAIRAWASKLAAQL
                     RPQPVLADTSTPALVGG"
     gene            complement(665..1642)
                     /locus_tag="VGT44_23970"
     CDS             complement(665..1642)
                     /locus_tag="VGT44_23970"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEV2583933.1"
                     /translation="MKTQDNPVIDQDFVGERDEAVATRENVPQQPPVWTGYAPGTPPP
                     WAWQPGANRPYPPRHPGRWPWVVLAFVLLILLISGVTAALFGVFGFTGYAGSATEKQT
                     FSVSPYPTLALTNDTGSIYVRAAPSGSAITIQATKHGSPWANLNDIQVTYTQNTTTNI
                     VIVNVNRLNSASFFTGLSVNFDVTVPSAAALQLKTNTGSIDVSGVSGQMALSSNTGSL
                     QVSSGTVRGNTALITNTGSVTFKGAIEQSGDYIFTTDTGSVNVTLPAESVFHVDASTD
                     TGSINTNFPGIVVQHRQVVGSDAHADVGSLPQATITLRTSTGSINLYEG"
     gene            complement(1697..2092)
                     /locus_tag="VGT44_23975"
     CDS             complement(1697..2092)
                     /locus_tag="VGT44_23975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583934.1"
                     /translation="MLSQQTLNTTPPVENIPMMQPPAAPRQAVPQYDAQYIEVLAQRL
                     MPRLMPEILYNLRNVEPARERNRAFGMSLALAIVSIALLIPLTAIVLGIVSTLGGGIT
                     AALIGISVIGFVLILINVLFNYTLFHERR"
     gene            complement(2313..3293)
                     /locus_tag="VGT44_23980"
     CDS             complement(2313..3293)
                     /locus_tag="VGT44_23980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583935.1"
                     /translation="MKRLYAGIATLILLVGAFILPSVASAASAPNTASPQNVAAANWS
                     LWSQVPGNGATYSALTTTNYANKLYVFARGTDNKIYVNSYNVRTGKWSLWSEVPGNGR
                     TYSALATTNYGNSLYLFARGTDNRIYVNSFNSRTGAWSLWSQVPGNGATYVGLATTNY
                     AGTLYVFARGTDNRIYVNSFNSSTGKWSLWSQVPGNGVTYARLATTNYEGRLYVFARG
                     TDNKVYVNSFNKDTGKWSLWSEVPGHGVTYSALGASNFEGQLYLFARGTDNKIYVNRF
                     NHDTGKWSLWSEVPGHGLTYVGLAATNYGDNLYLFARGTDNKIYVNVLHN"
     gene            3791..5941
                     /locus_tag="VGT44_23985"
     CDS             3791..5941
                     /locus_tag="VGT44_23985"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEV2583936.1"
                     /translation="MSTQQTGEEAKTMQKRSAFPLLLAVYGLLLLLSGCATVQTPPTS
                     TPTVGPSFTETTPLVMHTPLFAVNNVVWSPDGQRVAIANADGVFQVWEAATGRLISTH
                     DDPHRSLKISILGWSADGKRIISTILGPGTTVQVWDAATGATLATFSNSYPVAASHNG
                     KYVAVGSGSSVQIWETATSQQLSTFDTATTHVDQLAWSPDDRLLATTNSQNTADGAQD
                     FTIQVWQAATGQRLSTYQPPAHNRIFGLTWSPDSKRIVSTEGLGPTTNAVIGRAWDAT
                     SGMPLFSLQIATGQSSGLAWSPDGARIASWYNFSSEQAAQVWDAATGKLLYNTADQAV
                     ISVAWSPDGGQIAVVDQQANMRVLEAATGQTRLTYQEAANQRSVTWSPDGRHLASVGV
                     EGTTIWEAVADHRIASLAAHHQAERAVVWSPDGKSIAAEDGANLDAGFWDTAKGDSYG
                     DLQIYHENMFSLFNYRNAAAVAWSPDGAHVALSWASGYTDASSAQQVYVWGNAPTLCG
                     LDDSCPVSGGSHTAPITALAWSPDGKRIASASLDKTVLVYDVANQKNLLVYRGHTDAV
                     TAVAWSPDGKYIASASADKTVQIWDAASGKRVLTLSGHTGTVTAVAWSPDSRRVVSSS
                     EDGTVRTWDAVHGKLLLTYRGQSGAVITVAWSPNGHFIASAGADTTAQVWDANTGKTV
                     FVYRGHSDAILAVAWSPDSKRVASAGADGTLQIW"
     gene            complement(6033..7355)
                     /locus_tag="VGT44_23990"
     CDS             complement(6033..7355)
                     /locus_tag="VGT44_23990"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEV2583937.1"
                     /translation="MEQDTISGGTSVRKSAGGKAVSHQRLWWMELTRGIFTFAFGLLF
                     LAARSFAPRLFMYSLGAYLVVDGALELADLRRRKEDSRLKILDAAGGAMSLFAGLLSL
                     LFPTIAQFFLAGVIAIRLIIRSLSQVRAALRSHSPLSWVSSGFFALLGLFLLLFPLLA
                     ITLLVAFLGSYMLVAGLYLMLRGLALRFGSSGLPASPSRPPQAPPGLSDNLPLSTRRA
                     IVFVRRTAADGLGHIAWGFEWMNGWFNVGSVENLKSRLFANPEDMNFWSTHTLNPLAT
                     IQEREYPYDEYKLFFVTRPHPKDAWRTVIWESQQPYSFVHHNCNDVTYEILRTYGCKE
                     LLDPAKEYVPNDWYDALPGASYAITSQTAIPISLHQQSRREIATREIALAISPRMKGS
                     PPPRRAAHWRAWEELTLVWEMMIGHVLTLWTSGIKLIIQHRRSSVPRL"
     gene            complement(7454..8776)
                     /locus_tag="VGT44_23995"
     CDS             complement(7454..8776)
                     /locus_tag="VGT44_23995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583938.1"
                     /translation="MSTRHTNIDKDQEGAPQTPGSLAREDTAQREKASVTESFPRPPQ
                     RPLRAKRDRWFVIAAVIVTAALILSVFALAISLQGQHPGTQVTPTPTTPGATATPTLG
                     VSDAAYWDGILGTQGTNGKVESVSLGKVLGNPARQALVTVRHSDANRTLDVYVFDKIT
                     SAHPVQLFKLTGLVKGDAKISNVNTVITAEVDANSTLNAGKSSEQWTQDLFREFAWNQ
                     GEGTLVQVAFPGLFPDLTRYQAEADQASVDQGHQPWKNDPAQVAKALAVQFFQWNRSL
                     TTTITSGGGVHDVNATVRVQEAPGPDPLAPLPSVTVQLSRLEGNLQHMWVAIGVEDST
                     LLTLTTVEPRSLVTSPVKLAGIGTTYGANEGIIGPAKVLDHLYTVIGQTTLIGAVGLG
                     KTTYSTGVTYTSSFHAGVQEGIIAVYEANAGTDDEIASAVLVKVLLDP"
     gene            9133..>10266
                     /locus_tag="VGT44_24000"
     CDS             9133..>10266
                     /locus_tag="VGT44_24000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV2583939.1"
                     /translation="MMRGSLTPPATPAPSGERQAETHMLALKALFEQLQSSEQGLTGE
                     EARKRLDTYGPNDTTGIKRSSGLVQFLRLFLNPLVMILLIASIVSLVLGDRVNASIII
                     AIVLLSNILNFVQTYNSQHAVEQLRAGVAPTATVLRDGAWMETPRRDLAPGDMIRLTA
                     GDLVPADARLVQATDLHVQQAALTGESLPVEKHADDLDAPTESFAEAHNSVFLGTSVV
                     SGTATALVTATGRNTAFGDIAAHLASRPPETEFERGIKGFGLLIMQTVFFLVLFIFLV
                     GILGNHQPFETVLFAISLAVGLTPEFLPMITTVTLGQGALRMAKQKVIVKHLESIQNF
                     GSIDTLCSDKTGTLTSGETQLEGYLDLFKQASDRVLLFGYLNSS"
BASE COUNT         2057 a         3049 c         2948 g         2212 t
ORIGIN      
        1 gataccagca caccggcgct cgttggggga tgatcgaata gcgtggcgcg cgtggcgcaa
       61 gagcgctctt gcgccacgcg cgccacgcta ttcgatcatc ccccaacgag cgccggtgtg
      121 ctggtatcgg cgagaaccgg ctggggacgg agctgcgcgg ccagcttgct cgcccaggcg
      181 cgaatcgctt tccaatcccg cgcgtcgccc gcaggcatat ggttaaacgg gaagtggagc
      241 tgcgccgggt taatcatgcc gccgaacatc tcgacctcta ccggcgtcag ccaggggacc
      301 tgtgcaagcg cccggtcgag ttgctcacgc gaaccccgcc gatcttcctc ggcggcgctg
      361 agcgggccaa gtgcgaagat ggccacctgc cgctgcgaga gcgccgcgcg gtgccgcttc
      421 aggaacctgc gcgcgtcctt gtgccagcgg cccatgtaga gggctgcccc aagcacgacc
      481 gcgccgtagc cgtcgagcga acgcacctcc cgcgcgggtt gaatctccac ctccaatccc
      541 tccaggctga gcgtcgccgc gattgcctcg gcgacctctc gcgttgagcc gtatttcgta
      601 gcgtatgcta ccagaactgt cgttgtcatc gttgcctcct tatgtgtcca ggtgtctttg
      661 ttccttaccc ctcgtacagg ttaatcgagc cggtgctggt cctcaaggtg atcgtcgcct
      721 gtggtaaact tcccacatca gcgtgagcat ccgagccaac cacctggcgg tgctgcacta
      781 ctatacccgg aaagttcgtg ttgatcgaac cggtatcggt agatgcatcc acgtggaaaa
      841 cactctcagc aggcagcgtc acgttgaccg agccggtgtc tgtagtgaag atgtaatcgc
      901 cactctgttc aatcgcgcct ttgaatgtga ccgagccggt attcgtgatc agcgcagtgt
      961 tgcccctcac ggtcccagaa ctcacctgca gcgatccggt attgctcgag agcgccatct
     1021 gaccgctcac cccgctgaca tcgatgctgc ccgtgttcgt tttgagctgc aatgcagccg
     1081 cggacggcac tgtcacgtcg aagttgacgc tcaggccagt gaagaacgag gcggagttca
     1141 accggttgac gttcacgatg acgatattcg tcgtcgtatt ttgcgtgtag gttacctgga
     1201 tatcgttcag atttgcccag gggcttccat gcttagtagc ctggatagtg atcgcgcttc
     1261 cagaaggcgc cgcgcggacg tagattgagc cggtgtcgtt cgtgagcgcc agtgtcgggt
     1321 acgggctcac gctgaaggtt tgcttctcgg tggcgctgcc ggcatatcca gtgaagccaa
     1381 acacaccgaa gagggccgcc gtcacgccgc tgatcagcag gatcagcagg acgaacgcca
     1441 ggacgaccca gggccagcgt ccaggatggc gcggtggata tggcctgttt gctccaggct
     1501 gccaggccca tgggggaggc gtccccggcg cgtaccccgt ccataccggg ggctgttgtg
     1561 gaacattttc acgcgtagcg acagcctcat cgcgttctcc gacgaaatcc tggtctatga
     1621 caggattgtc ttgtgttttc atggttttac tccccaggcc agcctcccct catgacgagg
     1681 ggaggctggc ccgtgttcac cttctctcat ggaacagcgt gtagttgaac agcacgttga
     1741 tcaggatcag cacaaacccg atcacgctaa tgccgatcag cgccgcggtg attcctccac
     1801 ccagggtgga gacgatgccg agcacaatgg ccgtcagcgg gatcaacagg gcgatcgaca
     1861 cgatcgccag ggccagactc atgccgaagg cccggttgcg ctccctggct ggctcgacat
     1921 tgcgcaaatt gtacaggatc tccggcatca gtcgcggcat gaggcgctgc gcgagtactt
     1981 cgatgtactg cgcatcgtat tgagggaccg cctgccgggg tgcagcgggt ggctgcatca
     2041 ttggtatatt ctccacaggt ggagttgtgt tgagtgtctg ctgtgacaac atcgtattga
     2101 cctcctaatt accttccaaa ctcactttct ttcaagaaca cccatggtgt ccatcttcta
     2161 tcgtactgcg aaacaatcac tgatcgctct caaacttgtc ctgagaagac acagttctat
     2221 caccatttca agctcataag gaacgcctgg tgtattgcac accaggcgtt cctcactcct
     2281 ttggcatgtt gcgccaaagc gcaacaacgc acttaattgt gaaggacatt gacgtagatt
     2341 ttattgtcgg tgccacgcgc gaacaggtac aggttatccc catagttggt ggctgccagc
     2401 ccgacatagg tcaggccgtg tccaggcacc tcgctccaca ggctccactt gccggtgtca
     2461 tggttgaacc tgttgacata gatcttattg tcggtgccac gcgcgaacag gtagagctgc
     2521 ccctcaaagt tgctggcccc cagggcagag taggtcacac catgccccgg cacctcgctc
     2581 cacaggctcc acttgccggt atctttgttg aagctgttga cgtagacctt gttgtcggtc
     2641 cctcgtgcga acacgtacaa cctgccctca tagttggtgg ttgccagacg ggcgtaggtc
     2701 acaccgtttc caggcacctg actccacagg ctccatttac cggtgctgga attgaaactg
     2761 ttgacgtaga tcctgttgtc ggtccctcgc gcgaacacgt acaacgtgcc ggcgtagttg
     2821 gtagttgcca ggccgacata ggtcgcgccg ttccccggca cctgactcca caggctccat
     2881 gcgccggtgc gggagttgaa gctgttgacg tagatcctgt tgtcagttcc ccgcgcgaac
     2941 aggtacaggc tattcccgta gttggtggtc gccagggcgg agtaggtccg accgtttcca
     3001 ggtacctcgc tccacagact ccacttgcca gtgcgaacgt tgtagctgtt gacgtagatt
     3061 ttgttatcgg tcccccgcgc gaacacgtac aacttgttag catagttggt agttgtcagg
     3121 gccgagtagg tcgcgccgtt tcccggcacc tggctccaca ggctccaatt tgcagccgcc
     3181 acgttctgtg gtgaggctgt attaggagca gaagccgcgg aagccactga tgggaggata
     3241 aacgctccca ccaacaggat cagcgtagct atccctgcat ataaccgttt catctgaaag
     3301 gtcctttctc tttgatgctc actgaaactg ccctttccca aaggacagaa ttgatcgcga
     3361 tcccctcatt ttctctgtgt aagttgagag catgaggtga gactgtcatg tagcagtccg
     3421 ggaggtcgtt ggagacttta gcccgtcgtg tttacacgat agcacgttga tttcactatg
     3481 taatcacatt tttattgaaa aaagacacat ataaatcaca gtattacaag atatagtcac
     3541 atttctatca cagcaatact atgagattta ttcatgtgtt ttattcatag tagtaggagc
     3601 tgcagatctt tccctttcct gacatcgcag tctccctctg ccccttacta atttgtccag
     3661 gggacacaca tgaactgtga ccttgttgtg cctgttctcc cgtcttgtgt gactgctttg
     3721 agaggtaccc gttttacact tatctgcatt ggcgatctat ttgggcgagc tattaataag
     3781 gaggactgct atgagtacac aacaaaccgg tgaagaagca aaaacgatgc agaaacgatc
     3841 tgctttccct ttgttactcg cggtctatgg tttgctcttg ctcctgagcg gttgtgcgac
     3901 cgtccagaca cctcctacgt ctacccctac cgtcggcccc tccttcactg aaaccacgcc
     3961 cctggtgatg catactccgc tgtttgccgt caataatgtc gtctggtcgc ctgatgggca
     4021 gcgcgtcgcc atcgccaacg cggacggcgt cttccaggtc tgggaggctg caactggccg
     4081 gctcatctcc acccatgacg atccacatcg ctcgctaaag atctccattc ttggatggtc
     4141 ggcggatggc aagcgcatca tctcaacgat cctcggtccc ggcacgaccg tgcaggtctg
     4201 ggatgccgcc accggcgcca cactggcgac ctttagcaat tcgtatcctg tggccgcttc
     4261 acacaacggg aagtatgtag cagtcggaag cggcagcagc gtgcaaatct gggagacggc
     4321 caccagccag cagctttcca cctttgacac ggctaccact catgtcgacc agcttgcctg
     4381 gtctcccgat gatcgcctgc tggccaccac caactcgcag aacacagcag atggcgcgca
     4441 agactttacc attcaggtct ggcaggccgc gaccggccag cgcctctcca cctatcagcc
     4501 tcctgcacat aaccggatct tcggactcac ctggtcgccg gacagcaagc gcatcgtttc
     4561 gacagagggg ctgggaccga ctacgaacgc agtcatcggg cgcgcctggg atgcgacttc
     4621 tggcatgccc ctgttttcgc tgcaaattgc taccgggcaa tcttccggcc tggcctggtc
     4681 gcctgatggc gcgcgcatcg cctcctggta taatttctct tcagagcagg cagcgcaggt
     4741 ctgggatgcc gcgacgggta aactgctcta caacacagcg gaccaggccg tcatctcggt
     4801 tgcctggtcg cccgatggcg gccaaatcgc cgtagtagat caacaggcga acatgcgcgt
     4861 cctggaggct gccaccggcc agacacgtct cacgtatcaa gaggcagcca accagcgcag
     4921 cgtcacctgg tctcccgatg ggcgacatct ggcgagcgta ggcgtggagg gaacgacgat
     4981 ctgggaagcc gtcgcggatc accgcattgc ttctcttgcg gcacaccacc aggcggaacg
     5041 cgcggtggtc tggtcgcccg atggcaaatc gatcgccgcc gaggatggcg caaacctgga
     5101 tgcaggcttc tgggataccg ccaaaggcga tagttacggc gatctccaaa tctatcatga
     5161 gaacatgttc agcctcttca actaccgcaa tgctgcggcg gtggcctggt caccagatgg
     5221 tgcgcacgtt gccctcagct gggcctccgg ttacacggat gcctcatcag cacagcaggt
     5281 ctacgtctgg ggcaacgccc cgaccttgtg cggcctggac gatagctgtc ctgtgtctgg
     5341 cgggagccac acagcgccga ttaccgcgct cgcctggtcg cccgatggta aacgcatcgc
     5401 ctccgccagc ctggataaaa ctgttctggt ctacgatgtt gccaaccaga aaaatctgct
     5461 tgtctaccgg ggtcacacgg atgcggtgac ggcagtggcc tggtcgcccg acggcaaata
     5521 cattgcttcc gccagtgcgg ataaaaccgt tcagatttgg gatgcggcaa gcggcaagcg
     5581 cgtactcaca ttgagcggcc atacaggaac ggtgacggcg gtggcctggt cgcccgatag
     5641 caggcgcgtc gtctccagca gtgaagatgg cacggttcgc acctgggacg cggttcatgg
     5701 caagctgctg ctgacctatc gcgggcagag cggcgcggtc ataacggtgg cctggtcgcc
     5761 caatggccac tttattgcct cggcaggagc ggatacgacg gcgcaggtct gggatgcgaa
     5821 cacgggcaaa acggtctttg tgtaccgcgg ccatagcgat gccatcttag ctgtggcctg
     5881 gtcgcctgat agcaagcgcg ttgcctcggc aggcgcggat ggcacgctcc agatctggta
     5941 agagcgcgct gccctgttct gtctacgtca catagcgcta gagaatcacc tgttctctca
     6001 tggcgtgtcg cctcctccac cagtaggtca ggttatagac gaggcacact gctgcgccgg
     6061 tgctgtatga tgagcttgat acccgaagtc cacaaggtaa ggacgtgtcc aatcatcatc
     6121 tcccacacca gcgtgagttc ttcccaggcg cgccagtgcg ccgcgcgcct gggcggcggc
     6181 gatcccttca tacgcggcga gatcgccagg gcaatctcgc gcgtcgcgat ctcgcgccgc
     6241 gactgctggt gcagggagat cggaatggca gtctgcgatg taatggcata gctcgcgccg
     6301 ggcagggcgt cgtaccagtc gttggggaca tattccttgg cgggatcgag caactccttg
     6361 cagccatatg tgcgcaggat ctcgtaggtc acgtcattgc agttatgatg cacgaacgag
     6421 tagggctgct ggctttccca gatgaccgtc ctccaggcgt ctttcgggtg gggccgtgta
     6481 acaaagaaca gcttgtactc atcgtaggga tactcccgct cttgtattgt cgccaatggg
     6541 ttcagggtat gcgtggacca gaagttcatg tcttccgggt tcgcaaagag tctgctcttg
     6601 aggttttcga ccgaccccac attaaaccag ccgttcatcc actcaaagcc ccaggcgatg
     6661 tgtcccagcc cgtctgccgc tgttcggcgg acgaagacga tggcgcggcg cgttgagagg
     6721 gggagattgt cgctcagacc tgggggagcc tggggcggtc gagaggggga ggcgggaagg
     6781 ccgctggacc cgaagcggag ggcgagacct ctcagcatca aataaagccc ggcgaccagc
     6841 atgtagctgc cgagaaaggc gaccagcaga gtgatggcaa gcaggggaaa cagcagcagg
     6901 aagagaccca gcaaggcgaa gaagccactg gaaacccagg agagggggct gtgcgagcgt
     6961 agagccgctc gcacttgtga caggctcctg atgatgaggc gaatcgcgat gacaccggcg
     7021 aggaagaact gcgcaatggt tggaaacagg aggctgagca gccctgcgaa gagactcata
     7081 gcccctccgg cggcatcaag aatcttcagg cgagagtctt ccttccttct gcgcaagtca
     7141 gcgagttcaa gcgcgccatc gacgacgagg taggcgccca ggctgtacat gaagagacga
     7201 ggagcgaagg acctggcggc gaggaagagc aggccgaatg cgaaggtaaa gatgcctcgt
     7261 gtgagttcca tccaccagag ccgctggtgt gatacagctt ttccgcctgc gctcttccgc
     7321 acgctcgtcc caccgctgat ggtatcttgc tccatatctg cctcaatctt atccgcattc
     7381 gtcaaatcgg cgcccggtac gaagcttttt gcgcctggtc aggcattggc agcggcccca
     7441 gcgccgctcc cggctacgga tcgagcagca ccttgaccag cactgcgctg gcaatttcat
     7501 cgtcggttcc cgcattggct tcatatacgg ctatgatccc ttcctggaca cccgcgtgaa
     7561 agcttgaagt gtaggtcact ccagtcgaat aggtcgtttt ccccaggcca acagcgccga
     7621 tcagggtggt ttgaccaatg acagtgtaca ggtggtcaag caccttagcc gggccgatga
     7681 tgccttcgtt cgcaccgtat gtcgtcccga ttcctgcgag cttcactgga ctggtgacga
     7741 gtgaacgagg ctcaacggtg gtcagggtga gcagggtgct atcttccacg ccgatcgcta
     7801 cccacatgtg ttgaaggttg ccttcgagac ggcttaactg caccgtgacg ctgggaagcg
     7861 gcgccagagg gtccggtccg ggggcttcct gcacgcgcac agttgcgttg acatcatgga
     7921 cacccccgcc actggttatg gtagttgtca ggctgcggtt ccactgaaag aattgcacgg
     7981 ccagcgcctt cgcaacctgg gccgggtcat ttttccaggg ctggtgtccc tggtcgacac
     8041 tcgcctggtc ggcttcggcc tggtagcggg tcaggtcggg gaagaggccg ggaaaggcca
     8101 cctgcaccag tgtaccctcg ccctggttcc aggcaaattc gcggaagaga tcttgcgtcc
     8161 attgctcgct gctcttgccc gcgtttaggg tagagttggc atccacttct gcggttatca
     8221 cggtattgac attgctgatc ttcgcatcgc ctttcacgag gcccgtcaac ttgaagagct
     8281 ggacgggatg cgcgctggtg atcttgtcga agacgtagac atccagcgtg cggttcgcgt
     8341 cgctatggcg cactgtgacc agcgcctgcc gcgccgggtt ccccagcacc ttgcccaagc
     8401 tcacgctttc gactttccca ttggtcccct gggtgccaag aattccatcc caataggccg
     8461 catcgctcac gcccagcgtg ggtgtcgcgg tagcccctgg cgtcgttgga gtgggcgtga
     8521 cctgcgtgcc agggtgctgt ccttgcagcg aaatggcgag cgcaaagacg ctcaaaatga
     8581 gcgccgccgt cacgatcacc gcagcaatca cgaaccaacg atctcttttc gcgcgcaatg
     8641 gccgctgagg cggtcgagga aagctctcag tcacagatgc cttttcgcgc tgcgccgtat
     8701 cttctctggc taatgaacca ggtgtctgag gagcgccttc ttgatccttg tctatatttg
     8761 tatgtcgtgt actcatggat ttcctccttg caaaacctgc gcagattcac tgacgatcca
     8821 agcgtagtat acaggctgga gttcacaaat cagtcgcatt atgaatacgg agaatcacag
     8881 gaagatcact atttcctacc cgcaatgcca ttgattcccc ataagtgtgt ttgtgattgt
     8941 cctgtgatag ccaggcggcc tgttgtgata aagaagtgag cgtactcttt ttacgggaag
     9001 tgaaataaaa cagaatagta gcgcccagac acaatatgtg atacaactga tatcggcggt
     9061 tcatgcatgg cgcctgctca ccgacacggg cagccctatc gagttcattc tcttaaagcg
     9121 attctggaga tcatgatgcg aggctcactc actccaccag caacaccggc cccttccggc
     9181 gaacgtcaag cagagacgca catgctcgcg ctgaaagccc tatttgagca acttcagagc
     9241 agcgaacagg ggctgaccgg cgaggaagcg cggaagcggc tggataccta tggcccgaac
     9301 gatacgaccg gcatcaaacg cagttcgggt cttgtccaat ttttacgcct gttcttgaac
     9361 ccgctggtca tgatcctgct gatcgccagc attgtctcgc tcgtcctggg cgaccgcgtc
     9421 aacgccagca ttatcatcgc cattgtgctg ctcagcaata tcctcaactt tgtgcagacc
     9481 tacaactcgc agcacgcggt cgagcaattg cgcgcagggg tggcgcccac agcgacggtg
     9541 ctgcgcgatg gcgcgtggat ggaaacgcca cgccgggacc tggcgccggg cgatatgatc
     9601 cgcctcaccg ctggagacct ggttcccgcc gatgcgcgcc tggtgcaggc caccgaccta
     9661 catgtccaac aggcagcact gaccggcgaa tcgctgcccg tcgagaaaca cgccgatgat
     9721 ctggacgccc ccaccgagag tttcgccgaa gcgcacaata gcgtctttct tggcacctcg
     9781 gtcgtgagtg gcacggccac cgccctcgtt accgcgacgg gtcgcaacac ggcctttggc
     9841 gatattgctg ctcacctggc cagtcgtccg ccagagaccg agttcgagcg cggcatcaaa
     9901 ggctttggcc tgctgattat gcagaccgtg tttttcctgg tactctttat cttcctggtg
     9961 ggcatcctcg gtaatcatca acccttcgag acggtgcttt tcgccatctc gcttgccgtt
    10021 ggcctgacgc cggagttcct gcccatgatt acaactgtga cgcttggaca gggcgctctc
    10081 cgcatggcaa aacaaaaggt gatcgtcaaa cacctggaat cgatccagaa ctttggcagc
    10141 atcgacaccc tgtgcagcga taaaacgggc accctaacca gtggcgaaac gcaacttgaa
    10201 ggctacctcg atctcttcaa gcaagcatcc gaccgtgtgc tgctcttcgg ctacctgaat
    10261 agttcg
//
LOCUS       DASZAK010001369         2617 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_140460, whole genome shotgun sequence.
ACCESSION   DASZAK010001369 DASZAK010000000
VERSION     DASZAK010001369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140460"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VGT44_24005"
     CDS             <1..1024
                     /locus_tag="VGT44_24005"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEV2583940.1"
                     /translation="VGVGVWRFATGGINTSKVAVAQLLQRYSSKIVPPPTPNYGAIQS
                     APNLSSEITSNDQFYVVSKNLYADPVVDGTKWQLTVNGEVERPFTLNYNELMAMPLQA
                     QSEALMCISNDVGGPYMSNALWEGIPLATFLQRAGVKTGATKVVFHADDDYTDSIHLS
                     KALEPTTLLAVRMNGVTLPQEHGFPVRMLVPGIYGMKHCKWLTRIEIVNYDFLGYWQQ
                     RGWDDAAPVRLTSRIDTPMDGSTISVNRTAYIAGVAFSGNKGISEVDVSLDSGQTWQR
                     AILMQPSSDLTWVIWEYAWRPTTGNNYIITVRAIDLEGNVQDPREAPPLPIGSSGYHS
                     IGVLVA"
     gene            1213..1812
                     /locus_tag="VGT44_24010"
     CDS             1213..1812
                     /locus_tag="VGT44_24010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEV2583941.1"
                     /translation="MANMVMDAQVVERLRRHEETAFQALFERLQQPLTGYLHRLVGER
                     EVALDLAQDTFLQVYKEIGRTSGDLKLDAWIYRIATNYGLRYLNRKRLKQFIHLDSHE
                     LPDSALAMPGPEEQALTRVLVQQALGHMNPKDAAIVELHFGNGFTYEEIGQILQISAE
                     AVRKRVGRSAEKFRALYDESPVAPADSVETRLVAQKKRW"
     gene            1835..>2617
                     /locus_tag="VGT44_24015"
     CDS             1835..>2617
                     /locus_tag="VGT44_24015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583942.1"
                     /translation="MEKQHEQRDPGKNHPVDTGHLLALIRNQHFENEAFVSQHLAHCA
                     RCQQNYATLIQTSKTLDVLGQIAQSQRYPELTTAQLMKNARLGYSQRRFSGRRAARYA
                     YDGTRSPMRLMSLPFALALILLTIVIMITLALVGIGKISQQLNGSGNNVHTGTNPFST
                     GIVAQMPTPSTTPVLTLTPGAVSQPTLTATSSAARFISNCTTPFDKAHHFLDICGSNF
                     TPGDKVSLLVAYFSRPAQPFITVKVSASGTFDDRVYIPSCKDG"
BASE COUNT          628 a          727 c          696 g          566 t
ORIGIN      
        1 agtgggggtg ggagtctggc gcttcgccac tggcggcatc aacacctcaa aggtagccgt
       61 tgctcaatta ttgcaacgct attcaagcaa aattgtgccg ccgcctacgc cgaattatgg
      121 cgctatccag tccgcgccca atctgtcgtc agaaattacc tccaatgacc agttttacgt
      181 cgtctccaaa aacctgtacg ccgatcccgt tgtggatggc acaaaatggc aactgaccgt
      241 caatggcgag gtggaacggc cattcaccct caactacaac gaactgatgg cgatgccgct
      301 tcaggcgcaa agcgaagcgc tcatgtgcat cagcaacgat gttggcgggc catatatgag
      361 caatgccctg tgggaaggca taccactggc tactttcttg cagcgcgcgg gcgtcaaaac
      421 cggcgcaacc aaagtggtct tccacgccga tgacgattat accgatagca tccatctctc
      481 taaagccctg gagccgacaa ccctcctggc ggtgcgcatg aacggcgtca ccctgccgca
      541 ggaacatggc ttccctgtgc ggatgctcgt gccgggcatc tatggcatga agcattgcaa
      601 gtggttaacc cgcattgaga tcgtcaacta tgacttcctg ggctactggc agcagcgcgg
      661 ctgggacgat gccgcgccag tccgccttac ctcgcgtatc gacacaccca tggatggcag
      721 taccatctcg gtcaatcgca cagcatatat cgcgggcgta gccttttccg gcaataaggg
      781 catcagcgaa gtcgatgtca gcctcgactc cggccagacc tggcagcgcg ccatcttgat
      841 gcagccctcg tccgacctca cgtgggtgat ctgggaatac gcctggcgcc ccacgaccgg
      901 caacaactac atcatcacag tgcgcgccat cgatctggaa ggcaacgtcc aggaccccag
      961 agaagctccc cccttaccta ttggctcatc ggggtaccat agcatcggtg tcctcgttgc
     1021 ataagtaggg ggtagaatca atctatccac acctataaca tcaggagcaa ggcgttattg
     1081 tagtggcgac ccttgcggtc gccatgctaa caatgaaaca cataattcct gtccgctttt
     1141 gaataccaca ttgattgtat taaatgaggg cgctgcattt ttgcaagtgc ttatcgggaa
     1201 ggccgctggg ggatggccaa tatggtgatg gatgctcagg tggtagagcg actacgcagg
     1261 catgaagaga cagcctttca agcgttgttt gagcggctgc aacaaccgct gaccggatac
     1321 ctgcatcgcc tcgttgggga acgagaagta gctcttgatc ttgcgcagga tacttttttg
     1381 caagtgtata aagaaatagg aaggacttcg ggcgatctca aactggacgc ctggatctat
     1441 cgtattgcca cgaattatgg cctgcgctac ctgaatcgca aacgcttgaa gcagtttatc
     1501 cacctggata gccatgagct tcctgacagc gcgttggcga tgcccggccc ggaagaacag
     1561 gcactgacgc gtgtactcgt gcagcaggcg ctcggtcaca tgaacccgaa agacgctgcc
     1621 atcgtagaat tgcactttgg caacggcttc acttacgaag agatcggaca gattctccag
     1681 attagcgcgg aagccgtgcg caagcgcgtt gggcgaagcg ccgagaagtt tcgtgcattg
     1741 tacgatgagt cgcccgtcgc gcccgcagac agcgttgaaa cccggctcgt ggcgcaaaaa
     1801 aagagatggt agatgaatta tggcaaggag taagttggaa aagcagcatg aacaacgcga
     1861 ccctgggaaa aatcatccag tcgataccgg ccatctgctt gccttaattc gcaatcagca
     1921 ctttgaaaat gaggcatttg taagccagca tctcgcgcat tgcgcgcgct gtcagcaaaa
     1981 ctatgccacg ctaatacaga ccagcaaaac gcttgacgta cttgggcaaa tagcgcagag
     2041 ccagcgctat cccgaactga cgacggcgca attgatgaag aatgcccggc tgggatactc
     2101 ccaacgccga tttagcgggc ggcgcgccgc ccgttatgcc tatgatggca cgcgctcgcc
     2161 aatgcgatta atgagcctgc cattcgcgct ggcactcatt cttttgacca tcgtcattat
     2221 gattaccctg gcgctggtgg gcattggtaa aatatcccag cagttaaatg ggtcgggaaa
     2281 taatgtccat acggggacca atcctttctc aaccggaatc gtggcgcaaa tgccaacgcc
     2341 atcaacgaca ccggtgctga cgctcacacc gggcgcggta tcccagccaa cattgactgc
     2401 gaccagttcg gcggctcgct ttatttcaaa ttgtacaacg ccctttgaca aagctcatca
     2461 cttcctggac atctgcggct ctaactttac accgggggat aaagtatcgc tgttagtggc
     2521 ctatttttcc aggccggccc agccatttat tactgtgaag gtgagtgcat ctggtacatt
     2581 cgatgatcgt gtatatattc cgtcctgcaa ggatggg
//
LOCUS       DASZAK010001370         1905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_567658, whole genome shotgun sequence.
ACCESSION   DASZAK010001370 DASZAK010000000
VERSION     DASZAK010001370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567658"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1882)
                     /locus_tag="VGT44_24020"
     CDS             complement(<1..1882)
                     /locus_tag="VGT44_24020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583943.1"
                     /translation="MAGITESDWYEVGGGESGYVAVRSDDPNIVYTGNYQGYIGRYDH
                     RSRQSRNIAIWPELASGWGAKDQKYRFQWTFPILLSPHDPNILYITGNYVFRSMDEGN
                     SWEIISPDLTRDDVSKMEPSGGPIAKDNTGAEYYGTIFAFAESPIKRGLFWAGSDDGL
                     IHISQDEGQTWQNVTPSELPEWALISIIEPSPHDPATAYMAATRYKLDDFQPYLFKTN
                     DYGQSWTQITNGMRGESHDESGTYARVIREDPVRRGLLYAGTETGIHVSFDDGEHWQS
                     LRLNLPVVPIHDMVIKDSDLVVATHGRSFWILDDVTPLRQVSEEIQHTSVHLFKPRST
                     TRFMTSWGFSRSPSSGRFYRNTGGKMVTSLRKQKPDGEPVDYNLDAGQNPPNGVIVTY
                     YFKEKPEGEVKLSFFDAQGDKVRTFSSEPPSASAPPSASASIEEVENGEETTVVAPSE
                     DAPPVQGKEKRKERKEPRVPKEQGANRFVWRMRYAPAKPVDGYVAAESALAGPVAAPG
                     TYEVRLTVGDATYSETFTIDKDPRVVATQADLEEQLALRLKIRDKLSETHDAVNMIRN
                     IRQQVEDWERRTQEREEHESITKLGKSLKEKLSTIENELIQSKAKSRQDTLNFPAKLN
                     AKL"
BASE COUNT          358 a          551 c          538 g          458 t
ORIGIN      
        1 ctaacttcgc gttgagcttg gccgggaagt ttaaggtatc ctgcctgctc ttagcctttg
       61 actgaatgag ttcgttttcg atggtagaaa gtttctcttt cagcgacttg ccaagcttcg
      121 tgatcgattc atgctcctct cgttcctgcg tgcgtcgctc ccaatcctct acctgctgcc
      181 ggatgtttct gatcatattg acggcgtcgt gagtttccga aagcttgtcg cgtatcttga
      241 gtcgcaaggc aagctgctcc tccagatctg cctgtgtagc cactacccgt gggtccttgt
      301 cgattgtaaa ggtttcgcta taggtcgcat cgccaacggt cagccggacc tcgtaagtac
      361 cgggagctgc gaccggccca gccagggcac tttctgctgc cacataacca tcgacaggct
      421 ttgcaggagc atagcgcata cgccagacaa agcggttcgc tccctgctct ttgggcacac
      481 gtggctcctt acgctctttc cgcttctcct tcccttgcac aggcggtgca tcctcgctag
      541 gggcaaccac agtggtttcc tccccattct ccacctcctc tattgaggcc gatgcggatg
      601 gtggcgcgga cgcagacggc ggctcgcttg agaaggttct gactttgtcg ccctgagcat
      661 cgaagaagct tagtttcacc tcgccttccg gcttctcttt gaagtaatag gtcacaatca
      721 ccccgttggg tgggttctgc ccggcatcaa gattgtagtc taccggttcc ccatcgggct
      781 tctgcttgcg gagagaagtt accattttgc cgccggtgtt acggtaaaac ctgccagaac
      841 tcggcgaacg agagaagccc caactggtca taaagcgcgt tgtggaacgc ggcttgaaca
      901 aatggaccga cgtgtgctga atctcctcgc tcacctggcg cagaggcgta acgtcatcca
      961 gaatccagaa tgagcgccca tgtgttgcca cgacgaggtc gctgtccttg atgaccatgt
     1021 catgaatcgg caccaccggc aggttcaacc gcagcgactg ccagtgctcc ccatcgtcga
     1081 acgaaacgtg tatgcccgtc tccgtcccgg catagagcag cccgcgccgc acagggtcct
     1141 cgcggatcac tcgcgcgtag gtccccgatt catcgtggga ctcgccacgc ataccattgg
     1201 taatctgcgt ccagctttgc ccgtagtcgt tggtcttgaa tagatatggt tgaaagtcgt
     1261 ccagcttgta acgagtcgcg gccatatagg cggttgccgg gtcatgtggc gaaggttcaa
     1321 tgatgctgat gagagcccat tcaggcaact cgctgggagt tacgttctgc caggtctgcc
     1381 cttcatcttg cgagatgtga atgaggccat cgtccgagcc tgcccagaaa agtccgcgtt
     1441 tgattggtga ctcggcaaag gcgaagatcg tgccgtagta ttccgctccc gtgttgtcct
     1501 tggcgatcgg gccgccggaa ggctccatct tactgacgtc gtcgcgggtg agatcggggc
     1561 tgatgatctc ccaactgttg ccctcgtcca ttgaacggaa cacatagttg cctgtgatat
     1621 agagaatgtt ggggtcgtga ggtgagagca gaattggaaa tgtccactgg aagcggtact
     1681 tctgatcctt agcgccccag cccgaggcca gttcgggcca gatggcgatg ttgcgggact
     1741 ggcggctgcg gtggtcgtag cggccaatgt atccctggta gttgccggtg tagacgatgt
     1801 tcgggtcatc agatcgcacg gcaacgtatc cactctcgcc acccccgacc tcgtaccagt
     1861 cggactccgt gatgccagcc atggatgagc ggctggccac gccga
//
LOCUS       DASZAK010001371         8983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_567672, whole genome shotgun sequence.
ACCESSION   DASZAK010001371 DASZAK010000000
VERSION     DASZAK010001371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567672"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VGT44_24025"
     CDS             <1..1002
                     /locus_tag="VGT44_24025"
                     /inference="COORDINATES: protein motif:HMM:NF013275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV2583944.1"
                     /translation="VQLASVDKTVVGVMGWPYSSRVADAISVLAQAHIPMISQTASSD
                     SFTNASRYFFRVAPPNLSQGKAGAQYAEQRLNAHKVVVWEDPNDPYSDSLAKAFSQQF
                     TGDHNTVLATEDYTVGKPDSFNQLLTKSETFNPDLIYFSGYANDASVLLTDLPTSGQY
                     ASLNILGGDALYEVQGYQSSAHAATARMRFTTFAYPDEWQALCSGGQKFACTLPAFFQ
                     DYTSAFDPNNQHVGNPYGYSRADGDAILSYDATFAILSAAQIELTGNKTTLTPADLLA
                     GLQQIKGAQAIQGVSGQIAFDSAGNPVNKAFVILRVAPGGFFQMDSVVGCFQKGGAC"
     gene            complement(1044..1301)
                     /locus_tag="VGT44_24030"
     CDS             complement(1044..1301)
                     /locus_tag="VGT44_24030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEV2583945.1"
                     /translation="MRVIVRVIPRSRRNVIEWEAGILKARLTAPPVDGAANEALLALL
                     ADKLGMPRRALSIVQGATSRQKVVEIDGLTAEEFEKKLSHQ"
     gene            complement(1318..1602)
                     /locus_tag="VGT44_24035"
     CDS             complement(1318..1602)
                     /locus_tag="VGT44_24035"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV2583946.1"
                     /translation="MLGIPSDHGLLYGIVKYGIGLLIIALLIRVFAMWFGADERFAFI
                     RFLARMTDPFILPIRRIVRPVGVMDMSFLIAFFFLSTIEILLLQALPVGW"
     gene            complement(1609..2103)
                     /locus_tag="VGT44_24040"
     CDS             complement(1609..2103)
                     /locus_tag="VGT44_24040"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HEV2583947.1"
                     /translation="MNAEDMFFGRRIRQNHALEHATITILSGYIPDLRVSARSNSRGF
                     TIFGDVDLGQLRRALDEALRRLQAGEAQLAIHPNCGTNLVVGFSLGALGTLLGLYSSR
                     ASTRIASAAASSFAGVMAARPLGEYVQKHFTTLPDLQGVRVTEIFRRKVWGMTFVEVR
                     TMQE"
     gene            complement(2100..2846)
                     /locus_tag="VGT44_24045"
     CDS             complement(2100..2846)
                     /locus_tag="VGT44_24045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV2583948.1"
                     /translation="MNLEVISVISVEQLADNITHVRDAIAQAAERAARDPREITLVAV
                     SKTMPLDLVRMAYNLGVTHFGENRVQDALPKIEAFHAQDVSWHMIGHVQSNKARKVVG
                     PFALVQSVDSPRLAELLNRHASEQGMRLPILLQVNVAGEASKEGVSPDEALVLAQRVV
                     ELPGLRVEGLMTIAPIAQHPEDTRPVFRQLRLLREHLRETVPQGEWQHLSMGMTDDYR
                     VAIEEGATIVRIGRAIFGERIQTIAERARQ"
     gene            complement(2843..3769)
                     /gene="pgeF"
                     /locus_tag="VGT44_24050"
     CDS             complement(2843..3769)
                     /gene="pgeF"
                     /locus_tag="VGT44_24050"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEV2583949.1"
                     /translation="MIERSQGDTCHVQFALFQRFSELVHGSFMRTGGCSPPPFDSLNV
                     SVGAGDEVEHVLSNRLLALRSLGLDGYRCATAWMIHSADVLTLDGDEWEDWRDDWPHR
                     SYLVDGQELNWTMRPRQKADAVITRRRGVTLAMSSADCAPLLLFDPVQQAIGLVHAGW
                     RGTARGIAAIVVDAMREQFGCQPEQIFAGIGPSIGPCCYEVGNEVHRLFLGDTDFEVM
                     PTRGAYRNLVRESAVFTVQAVDDRGTGERQERLRLDLWETNRNQLLLAGLAPDHIEVA
                     GICTSCEKARFFSYRGEHRKTGRFPTLLALRP"
     gene            3900..4124
                     /locus_tag="VGT44_24055"
     CDS             3900..4124
                     /locus_tag="VGT44_24055"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HEV2583950.1"
                     /translation="MQIVKLRKVGNSTTVTLPSSVVEALHLHENDEIAIEVAGDRIVL
                     TRATPDFEDAWAAYQAVEPRYRNANRKLAE"
     gene            4130..4519
                     /locus_tag="VGT44_24060"
     CDS             4130..4519
                     /locus_tag="VGT44_24060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV2583951.1"
                     /translation="MIRFLSEEIVLAIHDDLIRLYGGVYGVRDEGMLDSAIQMPRATF
                     DGEFLHPTIFHMAAAYGFHLCQNHPFLDGNKRAAGMAMFTFLKLNGLNPIATEIDYYQ
                     TMMAVASGQIGKEDLAGWLETVVEPGR"
     gene            complement(4516..5622)
                     /locus_tag="VGT44_24065"
     CDS             complement(4516..5622)
                     /locus_tag="VGT44_24065"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV2583952.1"
                     /translation="MAPMKPRRRKSRKGRVILWSVLLFLLVVGGGVAYGIYYIESNVL
                     IPASHFVHPVGRSQDEPPVPTYSESAITSHAWNILLMGSDNDGKYNFPALLTQVMMLI
                     HIDPVNNQVWMVSIPRDSWVYVPDIGGMHKIDQAFFLGATQNNSFDDGVREARLTVEK
                     DYGVTIDRYAWVGLSGFASVIDTLGGVDVDVTHPIVDDAYPDDTGANANDPYAYERLY
                     LAPGPQHLTGEQALEYVRSRHADLIGDIGRTERQQQILSALKKKLTVTNIFTNLKTII
                     ADLQGKVYTDLSETEMLAFANFGRSLSDNAIQRLTLGPGQGNQDYGDFATVYDPSVGA
                     DQSVVIPNCVNIQPIINQVFALGNTQSCNVTGAG"
     gene            6054..7238
                     /locus_tag="VGT44_24070"
     CDS             6054..7238
                     /locus_tag="VGT44_24070"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEV2583953.1"
                     /translation="MNLTEIQQALQSERLDGWLFYDFRKSNPIAFQVLSLPLDGFFSR
                     RWFYYIPARGEPTRLVSSVESHVLDKLTGARLVFRTWQELQAHLGSILSQAKRVAMEY
                     SPMNAIPYLSRVDAGTIELVRACGVEVVSSADLAQRFGAQLSQAQMESHREAGRRLVA
                     AKDTLFSAIGRDLREGIEIDEYSTLQRFAALIEQAGLVVEQGLPHVAINANASNPHYE
                     PSAAHHSPIRRGDLVLFDFWASLPGSDSIIADYTWMAFAGTREEIPARQREVFAIVSR
                     ARDAAIDFARGRMAAGERVEGHEVDDAARRVIADAGYGDYFIHRTGHSIGRVVHADGA
                     NMDNYETRDERTLLPSTCCSIEPGIYLPEFGIRSEVNMLIHERDVEVTGVPAQQEIVA
                     LM"
     gene            7358..8836
                     /locus_tag="VGT44_24075"
     CDS             7358..8836
                     /locus_tag="VGT44_24075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583954.1"
                     /translation="MGDEWQQGPNSGGPAQDDLDKRLDDIRRKVIESTSEAQQRLKRA
                     VDKAGSYWQQSNTPLQPRQATSAEEDRIRRLANTWSLGNWQLARELGTYMELISWTDD
                     EIWEVTTQTRWETRSMEAVAEPYTGRPVGKIEPLLPVWDYQLPAVTGLKAPETRTRMN
                     GQDEVFACTACNSTGRLLCATCTGRGWIVCPDCKGRLRIRCSTCRGRGYIADWQDAKK
                     RSFFQRQADNLTKPVNEKVSDMFEGLRSQGLPIGNPVDADPASKGPTVPCPDCVSGEV
                     ECTCGTGKRVCSSCQGSKTEICSHCNGTGKVVRHREIVRRFTFSTQSQYIGATAIPEA
                     RLEKVSGDSVYDAEITEPLYADAAPDGVPMDVWRAAVQLVQQASANTTSATNPPGPRE
                     ETRASLQVLELVRIPYTKVDYRYGDQDYVFYIYDTVGQEKFYSDRYPARWDRIERLVR
                     SITADLMTPAQGAAQANAASDQVRGYRVPVEKPPYSITEEEE"
BASE COUNT         1944 a         2740 c         2579 g         1720 t
ORIGIN      
        1 gtgcagctcg ccagcgtgga caaaaccgtc gtcggcgtga tgggctggcc gtatagctcg
       61 cgcgtggccg acgccatcag cgtgctggcg caggcgcata tcccgatgat ctcgcagacc
      121 gcctccagcg actccttcac caacgcctcg cggtacttct tccgcgttgc tccgcccaat
      181 ctgagccagg gcaaagcagg cgcgcaatac gccgagcagc gcctgaatgc gcataaagtc
      241 gtggtgtggg aggacccgaa cgacccctac agcgatagcc tggcgaaggc gttcagccag
      301 cagttcaccg gcgatcacaa caccgtgctg gcgaccgagg actataccgt cggcaaaccc
      361 gacagcttca accagctgtt gacgaaatcc gaaacgttca accctgactt gatctacttc
      421 tcgggctacg ccaacgatgc cagtgtgctg ctgacagacc tgccgacatc cggtcagtac
      481 gccagcctga atatcctggg cggggacgcg ctctacgaag tgcaaggcta ccagagcagc
      541 gcgcacgccg cgaccgcgcg aatgcgcttc accaccttcg cctaccccga cgaatggcag
      601 gcgctgtgta gtggcgggca gaaattcgcc tgtacgctgc cggccttctt ccaggattac
      661 acttcggcct ttgatccaaa taaccagcac gtcggcaacc cctacggcta cagtcgcgcc
      721 gatggcgatg cgatcctctc ctacgacgcc actttcgcca ttctgtctgc cgcccagatt
      781 gagttgaccg gtaacaaaac cactctcact cccgcggact tgctggcggg cttgcagcag
      841 atcaaaggcg cgcaggccat ccagggtgtg agcgggcaga tcgccttcga cagcgctggc
      901 aacccggtta acaaagcctt cgtcatcctg agggtcgctc ccggcggttt cttccagatg
      961 gattccgtcg tcggctgctt ccagaagggc ggcgcttgct aaccccacca ataaaaacgc
     1021 ccctgctgtt tgcaggggcg tttttattgg tgagataact tcttttcgaa ttcctctgcc
     1081 gtcaacccgt cgatctccac caccttttgc cggctcgtag cgccctggac gatgctgagc
     1141 gcgcggcgcg gcatccccag tttgtcggcg agcagggcca gcagggcttc gttggccgct
     1201 ccatcaacgg gcggcgcggt caggcgcgct ttgaggatac ctgcctccca ctcgatgacg
     1261 ttccgccgac tgcgcggaat gacgcgcact atgacacgca taagtcgtgt ggcgctctca
     1321 ccagccgacc ggcagcgcct ggagcagcag gatttcgatg gtactcagga agaagaaggc
     1381 aatcaagaag gacatgtcca tgacgcccac cgggcgcacg atacggcgaa tgggaaggat
     1441 gaaaggatcg gtcatgcgcg ccagaaagcg gataaaggca aaccgttcat cagcgccgaa
     1501 ccacatcgcg aagacgcgaa tgagtagcgc gatgatgagc agcccgatgc catatttgac
     1561 gatgccatag agcaggccat gatcggaggg aatacctaac atactgcatt actcctgcat
     1621 tgttcgtact tcaacaaagg tcatgcccca cactttgcgg cgaaagattt cggtgacgcg
     1681 cacgccttgc aggtcgggca gcgtggtgaa atgcttctgc acgtattcgc ccagtggccg
     1741 cgccgccatc accccggcga acgaagaggc cgccgccgac gcgatgcgcg tactggcccg
     1801 gctggaatac aggccaagca aggtgccgag cgcgcccaaa gaaaagccca cgaccaggtt
     1861 ggtgccgcag ttgggatgaa tcgccagttg cgcctcgccc gcctgcaagc ggcgtagcgc
     1921 ctcgtcgagc gcgcggcgca gctgacccag gtccacatca ccaaaaatgg tgaagccgcg
     1981 cgaattggag cgcgcgctga cacgcaggtc gggtatatag ccgcttaaaa tggtgatcgt
     2041 cgcatgttcc agcgcgtgat tctggcgaat acggcggcca aaaaacatat cttcagcgtt
     2101 cattgccttg ctctttctgc tattgtttga atacgctcac cgaagatcgc ccgcccaatc
     2161 cgtacaatcg ttgccccttc ttcgatagcg acgcgataat cgtccgtcat acccatcgag
     2221 agatgctgcc actcaccttg aggcaccgtt tcccgcaggt gttcgcgcag caggcgcagc
     2281 tgccggaaaa cgggccgcgt atcctccggg tgttgcgcga tgggcgcaat cgtcatcagc
     2341 ccctcgacgc gcagccccgg cagctccaca acccgctgtg ccagtaccag cgcctcgtcc
     2401 ggcgacactc cctccttgct ggcctcgccc gcgacgttga cctggagcag aattggcagg
     2461 cgcatcccct gctcgctcgc gtgtcgattg agcaactcgg ccaggcgcgg gctatccacg
     2521 ctctgcacca gggcaaacgg cccgaccacc ttcctcgcct tgttgctctg cacgtgaccg
     2581 atcatatgcc agctcacatc ctgcgcgtgg aacgcctcga tcttgggcag cgcatcctgt
     2641 acgcggttct caccaaagtg cgtcaccccc agattatacg ccatccgcac aagatcaagc
     2701 ggcatagttt tcgagacggc caccagcgta atctctcgcg ggtcgcgcgc cgcccgttcc
     2761 gcagcctgcg caatcgcatc gcgtacatgc gtaatattat cggctaattg ctccacgcta
     2821 atcacagata tgacctctag gttcatgggc gcagcgccag cagcgttgga aaacgcccgg
     2881 tcttgcggtg ttcgccgcga tacgaaaaga agcgcgcctt ctcgcaactg gtacagatgc
     2941 ccgcgacttc gatatggtcg ggggccagcc cggccagcaa taactggttg cggttcgtct
     3001 cccataaatc gaggcgcagg cgctcctgcc gctcgcccgt cccacgatca tcgaccgcct
     3061 gtacagtaaa tacggctgac tcgcgtacca ggttgcgata cgcccccctg gtaggcatca
     3121 cctcaaagtc ggtatcgccc aggaagaggc ggtgtacctc gttgccgacc tcgtaacagc
     3181 aggggccgat ggacgggccg ataccggcga aaatctgctc cggctggcag ccgaactgct
     3241 cgcgcatggc atccacgacg atggcagcaa taccgcgcgc cgtgccgcgc cacccggcat
     3301 ggaccaggcc aatcgcctgc tggacggggt cgaagagcag cagcggcgcg caatcggcgg
     3361 aggacatcgc cagcgtcacc ccgcgcctgc gcgtgataac ggcatcggcc ttctggcgtg
     3421 gccgcatcgt ccagttcaac tcctgcccat caaccaggta ggagcgatgg ggccagtcgt
     3481 cgcgccagtc ttcccactcg tcaccatcca acgtcagcac gtccgcgctg tggatcatcc
     3541 aggccgtcgc gcagcgatag ccgtccaacc ccaggctgcg cagcgccaat aagcgattgg
     3601 aaaggacatg ctcaacctca tctcccgccc caaccgagac attcaaacta tcaaacggcg
     3661 gtgggctgca cccgccagtg cgcataaaac taccatgaac aagttcagag aatcgctgaa
     3721 agagggcgaa ctgtacatgg caggtatcgc cctgggatcg ttcgatcaag gtgtctcctg
     3781 gtatattgat attttatttc gttgggttta atggtattgt agcacacggc agaccggtat
     3841 tgacattccg caccccaaag tatacactat tgtatataca atgaaagtga ggtgcagaaa
     3901 tgcaaattgt gaaattgcgc aaggtaggca attctacaac cgttaccctt ccgtcctcgg
     3961 ttgtagaggc tctccattta catgaaaacg atgaaatcgc tattgaggtt gccggagatc
     4021 gtattgtgct tactcgtgct acaccagact tcgaggatgc ctgggctgcg tatcaggcgg
     4081 ttgaaccgcg ttatcgcaat gcgaatcgca agctagccga gtaagcgtca tgatacgctt
     4141 tctgtctgaa gaaatagtgt tggcgatcca tgacgacttg attcgtctct acgggggcgt
     4201 ctatggcgtc cgagacgaag gaatgcttga ttctgccatc cagatgccaa gagctacctt
     4261 cgatggcgag ttcttacatc caaccatctt tcacatggca gccgcctacg gttttcatct
     4321 ctgccagaat cacccatttc ttgatggaaa caaacgggcg gctggcatgg ctatgttcac
     4381 atttttgaaa ctgaatggat taaatcccat tgcaacggaa atcgattact atcaaacaat
     4441 gatggccgtt gcaagcggcc agataggtaa agaagacctg gcaggatggt tagaaacagt
     4501 agtagagcca ggccgctaac ccgccccggt cacattgcag ctttgtgtat tccccagggc
     4561 aaaaacctgg ttaatgatgg gctggatatt gacgcagttg gggatcacca cgctctgatc
     4621 cgcgccaacc gaggggtcgt agacggtggc gaagtcgccg taatcctggt tgccctggcc
     4681 ggggccgagt gtcaggcgct ggatggcgtt gtccgagagt gagcgaccga agttagcgaa
     4741 ggccagcatc tccgtctcgc tcaaatcggt gtagaccttg ccttgcaggt cagcgatgat
     4801 ggtcttcagg ttcgtgaaga tgttcgtcac ggtcagtttc ttcttgagcg ccgagagtat
     4861 ctgctgctgg cgttcggtgc gcccgatatc gccgatcagg tcggcatggc gcgagcgcac
     4921 gtactccaat gcctgctccc ccgtcaaatg ctgcggcccc ggcgcgagat agaggcgttc
     4981 ataggcgtag gggtcgttgg cattcgcgcc cgtgtcgtcg ggataggcgt catccacgat
     5041 ggggtgcgtc acatcgacat cgacgccgcc cagcgtatcg atcacgctgg cgaagccgct
     5101 caatcccacc caggcgtagc gatcgatggt gactccgtag tccttctcca ccgtcaaacg
     5161 cgcctcgcgc accccatcat cgaaactgtt gttctgggtc gcgcccagga aaaacgcctg
     5221 gtcgattttg tgcatacccc cgatatccgg cacgtacacc cacgagtcgc gcgggatcga
     5281 gaccatccac acctggttat tgaccggatc gatatggatc aacatcatta cctgcgttag
     5341 taaggcgggg aaattatact tcccatcatt gtcgctgccc atcagcaaaa tattccaggc
     5401 atggctcgta atggcgcttt cagaataggt tgggacgggt ggctcatcct ggctgcgccc
     5461 gaccgggtga acgaaatgcg atgccgggat aagcacattg ctctcaatat agtagatgcc
     5521 ataagccacg cccccgccca ccaccagtaa gaagagcagg acagaccaca gaattaccct
     5581 cccttttcgc gacttgcgcc ggcgcggctt cataggcgcc atcgtcattc ccggcaaggg
     5641 aggaggagga ggaggaggac ctgcatacgg tttttgagtt ggttgctcat tcatcaaatt
     5701 atgctcactt actaaagttc tttcaatata taaataccgt aggagccgat ttatcgcgca
     5761 caccgcctat tatgaatgtt cactagcaag agcggtaata ggatgtcatc ctgagcgcag
     5821 cgaaggatct cactcgccgg acacgcagat ccttcgctgc gctcaggatg acaagaccgg
     5881 ctttgtcgga aaccttcatt atcgcgtcct tgcccggggt cgagcaagga cgactggacg
     5941 cgatgaatcg catccctacg acacggtggg cgatgtacaa agcatgatac aatgccctca
     6001 agatagagca tacctccaaa agatcaaaaa acaatcaaga gaggacgttt ttcatgaacc
     6061 tgacagaaat tcagcaggcg ttgcaaagcg agcggctcga cggctggctt ttctatgatt
     6121 ttcgcaaatc caaccctatc gccttccagg tactctcgct gccgctggat ggcttctttt
     6181 cgcggcgctg gttttattat attccagcgc gtggagaacc gacacggctg gtcagttcgg
     6241 ttgagtcgca cgtgctggac aaattgacgg gggcgcggct ggtctttcgc acctggcaag
     6301 agttgcaggc tcacctggga agtatcctca gccaggcaaa gcgcgtggcg atggaatatt
     6361 cgcccatgaa cgcgatcccc tacctgtcgc gcgtcgatgc cggaacaatc gagctggtgc
     6421 gcgcctgcgg tgtagaggtc gtcagttcgg cggacctggc gcagcgtttc ggggcgcagt
     6481 tgagccaggc acagatggag agccaccgcg aggccggaag acgcctggtc gccgccaaag
     6541 acacgctttt ctccgctatc ggtagagacc tgcgcgaggg gatagagatc gacgagtaca
     6601 gcacgttgca gcgtttcgcc gcgctgatcg agcaggccgg gctggtggta gagcagggtt
     6661 tgccgcacgt ggccatcaac gccaacgcca gcaatccgca ttacgagccg agcgccgcgc
     6721 accacagccc aatccggcgc ggcgacctgg tcttgttcga cttctgggcc agcctgccag
     6781 ggagcgactc gatcatcgcc gactacacct ggatggcttt cgcgggaacg cgcgaggaaa
     6841 tcccggcgcg ccagcgcgag gtgttcgcga tagtgagccg cgcccgcgat gcggctatcg
     6901 acttcgcgcg agggcgcatg gccgctggcg agcgcgtcga ggggcacgaa gtggacgatg
     6961 ccgcccgccg cgtgattgca gacgcgggct atggcgacta cttcatccat cgcaccggtc
     7021 acagcattgg ccgcgtggtt catgccgacg gcgcgaatat ggacaactat gagacacgcg
     7081 atgagcgcac cctgctgccc tcgacctgct gctcgatcga gccgggcatc tatttaccgg
     7141 agttcggcat tcgcagcgag gtcaatatgc tcatccacga acgcgatgtc gaggtgacgg
     7201 gcgttccggc gcagcaggaa attgtcgcgt tgatgtaggc acgcacctac aaccttccaa
     7261 aagatttgac gtatatgtgt gtgataacac attgttgaac aggcgttgcg gtatcagtca
     7321 accgcccccg taacgccctt tagggaggca tacatcaatg ggtgatgaat ggcagcaggg
     7381 tcccaattct ggcggacctg cacaagatga tcttgataag cgccttgacg atatccggcg
     7441 caaagtaata gaaagcacat cggaagcgca gcagcggctt aagcgcgcgg tcgataaagc
     7501 gggtagctac tggcagcagt cgaatacgcc cttgcagccg cgccaggcca ccagcgcgga
     7561 ggaggatcgc attcgccgcc tcgcgaacac gtggagcctg ggcaactggc aactcgcgcg
     7621 cgaactcggc acctatatgg aactgatttc ctggacggat gatgaaatct gggaggtcac
     7681 gacccagaca cgctgggaaa cgcgcagtat ggaagccgtc gccgaaccct acacgggacg
     7741 cccggttggc aagatagaac cattgttgcc cgtctgggat taccagttgc ccgccgtcac
     7801 cggtctgaaa gcgccggaga cgcgcacacg catgaatgga caggacgagg tgtttgcctg
     7861 caccgcctgc aacagtaccg ggcgactgct ttgcgcgacc tgcaccgggc gcggctggat
     7921 cgtttgcccc gattgcaagg gacgccttcg cattcgctgc tcaacctgcc ggggacgcgg
     7981 ctacatcgcc gactggcagg acgctaagaa gagatcgttt ttccagcggc aggccgataa
     8041 cctgacgaag ccggtcaacg aaaaagtttc cgatatgttc gagggcctgc gttcgcaggg
     8101 tctgccaatc ggcaacccgg tagacgccga tcccgccagt aagggaccaa ccgtgccgtg
     8161 ccccgattgc gtcagcggcg aagtcgaatg cacctgcggc accggcaagc gtgtctgctc
     8221 gagctgccag ggttccaaga cggaaatctg ctcccactgc aacggcaccg gcaaagtggt
     8281 gcgtcaccgc gagatcgtgc gccgcttcac gtttagcacg cagtcacagt atatcggcgc
     8341 gaccgccatc cccgaagcgc gcctggagaa ggtcagcggc gactcggtct atgacgccga
     8401 aatcaccgaa ccgctctacg ccgacgccgc gcccgacggc gtgccgatgg atgtctggcg
     8461 cgccgccgtg caactggtcc agcaggccag cgccaatacc accagcgcga ccaacccgcc
     8521 cggcccgcgc gaggagacaa gggccagcct gcaagtgctc gaactggtgc gcatccccta
     8581 caccaaagtg gactatcgct acggcgacca ggattacgtc ttctacatct acgatactgt
     8641 tggccaggag aagttctatt ccgaccgcta cccggcgcgc tgggaccgca tcgagcggct
     8701 ggtacgctcc atcaccgccg acctcatgac ccccgcgcag ggggcagccc aggccaacgc
     8761 cgcgagcgac caggtacgcg gctaccgcgt cccggtcgaa aaaccaccct actcgatcac
     8821 cgaagaagaa gaatagtata caaaagatag caagacgccg gtgggctgcg cccaccggcg
     8881 tcttgctgtc ttttgtatac tatatgctag attgaagaag ggaacaaacc gatgaatgac
     8941 gaacaattga aacgctacct gctggccgtg ccgaaggccg aac
//
LOCUS       DASZAK010001372         2478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_318889, whole genome shotgun sequence.
ACCESSION   DASZAK010001372 DASZAK010000000
VERSION     DASZAK010001372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318889"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VGT44_24080"
     CDS             <1..712
                     /locus_tag="VGT44_24080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583955.1"
                     /translation="ETLVGYAQSAMMRVLDRVSAAEYRWSKRTSSVNQQSAHQRRTGE
                     VLTYAQLCLELTQRIGEQRLEEAIAAAWETMPAGLDSRGRCLHIARHLWSLSGKRGPA
                     IMIYYSPPFIPHVAATPCALHQAIHAIADAHPELQLLVQEYFPLISDMSYLRLDPGIE
                     TTALIANMPLWPDSPDQPARPGSYALPLEAMRQLDLPVVNLGPYGKGAHQAGERVLMS
                     YSFGVVPQLIWEVIERLA"
     gene            801..1622
                     /locus_tag="VGT44_24085"
     CDS             801..1622
                     /locus_tag="VGT44_24085"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV2583956.1"
                     /translation="MDSVSQPQGAPLPSRPIASARITVHPIEAIYFVIGLIVLITSAF
                     LVHAHPRPFDLDLQITQTIQGFPSIPVLTGLIEFVSEINNPTPSIIALAVWLVGLVVI
                     GFMARWRGMSSVKWFQSAASLILTVAIAGGLNLLINTLVGRPRPGSFGEHITVFSHIP
                     EKSFPSGHTEHDVAYYGFLLYLSFTPPVRQWRYRWVLIPFQAIAIFDILTIGYSRVLE
                     GEHWLTDVLGGYLSGSLWLLMCIVIYRYVTDLIAERLEEHGVPPSETVSTATSNI"
     gene            1727..>2478
                     /locus_tag="VGT44_24090"
     CDS             1727..>2478
                     /locus_tag="VGT44_24090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV2583957.1"
                     /translation="MATPISEGTLLWEPSEAVKQHSNMLRYMHWLEREKGLRFDTPDE
                     LWHWSVDNLEEFWASMWDYFQIKASRPYQSVLSDHAMPGAQWFSGAELNYAEHVFRNR
                     TNARPALIFQSETQPLTELSWDDLYQQVCTLAAALRTMGVQRGDRVVSNIPNIPQAAV
                     AFLASASLGAVWSSCSPDFGTRSVIDRFQQIEPKVLFAVDGYQYAGKTVDRRAIIAEL
                     QEALPTLQHTVIVPYAFNDTSAASYREAVLWT"
BASE COUNT          495 a          822 c          651 g          510 t
ORIGIN      
        1 cgaaacactt gtcggctacg ctcaatccgc catgatgcgc gtgctagatc gcgtcagcgc
       61 ggcagagtat cgctggagca aacgcacatc ttctgttaac cagcaaagcg cgcatcaacg
      121 gcgtaccggc gaagtgctta cctatgccca attgtgcctg gaacttacac agcgcatcgg
      181 cgagcagcgc ctggaagaag cgatagccgc cgcgtgggag accatgccag ccggactcga
      241 ctcgcgcggg cgctgcctgc acatcgcgcg ccatctctgg tcgctgagcg gcaagcgcgg
      301 ccccgccatc atgatctatt attcgccgcc cttcataccg cacgtcgccg cgacaccctg
      361 cgcgctgcac caggccatcc acgccatcgc ggatgctcac cccgaattgc aactgctcgt
      421 gcaggaatat tttcccttga tctccgatat gagctacctg cgcctcgatc caggcatcga
      481 aaccaccgcg ctcatcgcca acatgcccct ctggccagat tctcccgatc aacctgcgcg
      541 ccccggctct tacgcgcttc ctttagaggc catgcgacag ctcgatctgc ccgtcgtcaa
      601 tctcggcccc tatggcaaag gcgcgcacca ggcgggcgag cgcgtgctga tgtcctattc
      661 gtttggcgta gtgccgcaat tgatatggga agtgatcgag cgacttgcgt gaaattttgc
      721 cccttcgacc gtctatatca tagaatgccc gaaaaagaga gagccggtct ttctgcctga
      781 aaccaacaag gaggggacat atggacagcg tctcacagcc acagggagcg ccgctcccat
      841 cgcgtccaat cgcgtcggcg cgcatcacgg ttcacccaat cgaggcaatt tattttgtga
      901 tcggcctcat cgtactcatc acgtcagcct tcctggttca cgcgcacccg cgcccgtttg
      961 acctcgacct gcaaatcacg cagaccatcc agggctttcc atccattccg gttctgaccg
     1021 gcctgatcga attcgtcagc gagatcaata acccgacgcc ttccatcatt gcgctagccg
     1081 tctggctggt cggcctggtc gtgatcggct tcatggcccg ctggcgcggc atgtcgtcgg
     1141 tcaagtggtt tcaatccgcc gcgtcgctga ttctgacagt ggccatcgcg ggcggcctca
     1201 acctgctcat caacacgctc gtcgggcgtc cgcgccccgg cagctttggc gagcatatca
     1261 cagtttttag ccacatccct gagaaaagct tcccttcagg ccataccgag catgacgtgg
     1321 cctattacgg cttcctgctc tacctcagct tcacgccgcc cgttcgccag tggcgctatc
     1381 gctgggtgct gatccccttc caggccatcg ccattttcga catactcacg atcggctact
     1441 cgcgcgtcct ggagggcgaa cactggctca ccgacgtgct gggtggctac ctctccggct
     1501 cgctctggct gctgatgtgt atcgtgattt accgctacgt caccgacctg atcgccgagc
     1561 gcctggaaga gcatggtgtg cctccatcgg agacggtttc tacagcgaca tcgaacattt
     1621 agactgacca acgtgccagt aggcaggtgc gcttctcccc aaaatatgcc atactgtagt
     1681 ggaagcgtta tacaacctga gacgattgcg aggaaggtac tactgtatgg caacaccaat
     1741 cagcgaggga acgctgctct gggaaccgtc agaggcagtc aagcagcatt ccaatatgct
     1801 gcgctatatg cattggctcg aacgcgaaaa gggtctgcgc ttcgatacac cagacgaatt
     1861 gtggcactgg tcggttgata atctcgaaga gttctgggct tcgatgtggg actacttcca
     1921 gatcaaagcc tccagaccgt atcaaagcgt cctatccgac cacgctatgc ccggcgcgca
     1981 gtggttcagc ggcgcggaac tcaactacgc cgaacatgtc ttccgcaatc gaacgaatgc
     2041 gcgacccgcc ctgatctttc aatccgaaac gcagccgctc accgaacttt cctgggatga
     2101 cctctatcag caggtctgca cgcttgccgc ggccctgcgc actatgggcg tacagcgtgg
     2161 cgaccgtgtc gtctccaaca tccctaacat cccgcaggct gccgtcgctt ttctggcgag
     2221 cgccagcctc ggcgcggtct ggtcaagctg ctcgcccgac ttcggcacgc gcagcgtgat
     2281 cgaccgcttc cagcagatcg agccaaaggt actcttcgcc gtcgatggct accagtacgc
     2341 cggaaaaacc gttgaccgcc gcgccatcat cgccgaattg caggaagcgc tgccaaccct
     2401 gcaacacacc gtcatcgttc cctatgcctt caacgatacc agcgcggcca gctaccgcga
     2461 ggccgtcctc tggacaga
//
LOCUS       DASZAK010001373         3856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_212014, whole genome shotgun sequence.
ACCESSION   DASZAK010001373 DASZAK010000000
VERSION     DASZAK010001373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212014"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="VGT44_24095"
     CDS             complement(<1..480)
                     /locus_tag="VGT44_24095"
                     /inference="COORDINATES: protein motif:HMM:NF014615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEV2583958.1"
                     /translation="MEHILLETLRRQPVLCDGAMGSLLHARGITYERCFDALNLSYPE
                     VIAGIHREYASAGAQILETNSFGANRAKLEEYGLEEQVRVINMSAVKLAREAREVAGQ
                     PVFIAGAIGPTGRPLQAPDERRLLELRSIFREQIEALQEGGVDLLVLETFSSLAELRQ
                     "
     gene            complement(584..2137)
                     /locus_tag="VGT44_24100"
     CDS             complement(584..2137)
                     /locus_tag="VGT44_24100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEV2583959.1"
                     /translation="MQDAQQMLAPGALVRERYLVKSLIGRGNSGVVYLVEDQRASQSG
                     QQLFALKEISGLNKQERMQMTFNSIALRQIDHPTLPHVHHVFNDDRRNRVCIIIQYID
                     GPDLDTMRQHQAGRRFSWPEVKEMLDPIADALSYLHRQTPPIIHGDVKPVNILLAQPD
                     ERFMLVDIGVTRGSQSSSIHHFTGYKAPEQYGKSVDERADIYGLGATCYTLLTGIVPP
                     NALERGAHLERGQADPLKPVDALAPAVPSHVARAIHRSLSIYASERFASVENFWQALQ
                     TASREVESRVSDAALPATVNTPIPAGYAPDTLTGLLSRNRSYRHITAPIAFLKRFMVP
                     LALLAVIVLVSASVGIWALTQSHHAPASGVAPATTASVEPATPDPVGTRPLATPTSSP
                     GTYPSLVGSYAGTIADITVTSNGAPMILQGIHQVGGTIGGYFNAGPPISISGPFTGTI
                     DHAKNFHFIVRDAVGHPLLFIEGAIQSATYLSGDFYRCVAGAASGAPCARAPKGSGLW
                     TAELVKAGV"
     gene            2337..3197
                     /locus_tag="VGT44_24105"
     CDS             2337..3197
                     /locus_tag="VGT44_24105"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEV2583960.1"
                     /translation="MKRISVLGVGYLMLGLGLLGICGAALLGGPRSTASAQPAANPSF
                     VRVLHASPFVGTADVFVDGAKLLSSFAFGAVTGYATVPAGPHKVQIALVGKGIGAAAL
                     TQVLTVSPGFAYTVAAIGTSASNLSLQVFVDDNLLSPGTAKLRVYQLSPDGGTVTVSA
                     GGKTLLSGIGYQNASNYAVVAAGSYTFNVDSPANNATLTTSAALAANTVDSLFVVGMF
                     NGTPKSELVSSQAQGLPGLPNTGSDPNAISQIGSAQPFAPSPWSWPLGIASLLIIGCG
                     VYLRRVTAKR"
     gene            3219..>3856
                     /locus_tag="VGT44_24110"
     CDS             3219..>3856
                     /locus_tag="VGT44_24110"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HEV2583961.1"
                     /translation="MSQARRLLLARLLVCLSASLCGLAAVLLLSACLSNSTTASATGQ
                     SVVRASSGGPAPKASATPHPTPPTRPTVSPDTPLRLLIPAIGVNAPVEMLSFGANGDL
                     ATPTQHPWDDVGWYDQGPHPGERGSAVIDGHLDRPGGFPAVFWNLGHVQVGADVQVTN
                     STGKTLHFRVTGVMYYTPDQAPLQEIFGNQSGTYLNLITCAGDWIPSQHQTTL"
BASE COUNT          814 a         1112 c         1081 g          849 t
ORIGIN      
        1 ctggcgtaac tctgccaggc tggagaaggt ctccagcacc agcaaatcga cgccgccctc
       61 ctgtaacgcc tcgatttgct cgcggaagat actgcgcagt tccagcaaac ggcgttcatc
      121 gggcgcctgc aaagggcggc ccgtcggccc gatcgcgccc gcgatgaaca ccggctggcc
      181 cgcgacctcg cgcgcctcac gcgccagctt gaccgcgctc atatttatca cgcgcacctg
      241 ctcttcgagg ccatactcct ccagcttggc gcggttggcc ccaaacgaat tcgtttcgag
      301 aatttgcgcg cccgcgctgg cgtattcgcg gtgaatgccc gcgatcactt ctggataaga
      361 aagattcaac gcatcaaagc atcgttcata agtgatgcca cgcgcgtgta gcagcgatcc
      421 catcgcgccg tcacaaagca ccggctgccg tctcaaggtc tcaagtaata tatgttccat
      481 gttctatctc tggcaaaact ggctgcaagc tacaactctc gtgatgattg tgtctctatt
      541 ttacacccaa tttacctttt tagggaaaca ggatgcgtcc cgtctacacg ccagctttca
      601 ccagttcagc agtccagagt ccgctgcctt tgggggcgcg cgcgcagggc gctccagagg
      661 cggcgcctgc gacgcaccgg taaaaatccc cgctgagata ggtggcagat tgaatggcgc
      721 cttcaatgaa gaggagagga tgacccacag catctctcac gataaagtgg aagttttttg
      781 cgtgatcgat ggtacccgta aaggggccgc taatgcttat tggcggaccc gcgttgaaat
      841 accctccgat tgtgcctcca acctggtgaa tgccctggag gatcatgggc gccccattcg
      901 atgtaaccgt gatatccgcg atggtaccgg catacgagcc gaccaggctg ggataggtcc
      961 ccggcgacga agtgggcgtc gcgagtggtc gagtgccgac cgggtcgggt gtggctggct
     1021 ccacagaagc agtcgtggca ggcgcaaccc ctgatgcagg cgcgtgatgg ctttgtgtga
     1081 gcgcccagat accgacgctc gcgctcacca gcacgattac cgcgagcagg gcaagcggga
     1141 ccatgaagcg tttgaggaag gcaatgggag ccgttatgtg tcgatatgat cgattcctgg
     1201 acagcagtcc tgttagagtg tctggcgcgt atccggcggg aatcggcgtg ttgacagtgg
     1261 cgggcagcgc cgcatcgctc accctcgact cgacttctct tgaagccgtc tgcaacgctt
     1321 gccagaaatt ctctacagaa gcaaaacgct ctgaagcata aatagagaga gatcgatgta
     1381 tggcgcgcgc gacatgcgac ggaacagccg gtgccagcgc gtccaccggt ttcagcgggt
     1441 cggcctgccc tcgctcaagg tgcgctccac gctcgagcgc gtttggggga acaatgccgg
     1501 tcaacagcgt atagcaagtc gcgcccaggc cgtagatatc cgcgcgttcg tccacgcttt
     1561 tgccgtattg ctctggagct ttataaccgg tgaaatgatg aatggaactt gattgtgatc
     1621 ccctggttac gccgatatca acgagcatga aacgttcgtc tggctgagcc aataaaatat
     1681 tgactggctt gacatcccca tgaatgatgg gcggagtttg ccggtggaga taggagagcg
     1741 catcggcaat gggatcaagc atctccttca cttctggcca actaaaacgt cgccccgctt
     1801 gatgctgacg cattgtatct aaatcaggtc catcaatgta ttgtataata atacagactc
     1861 gattacgtcg atcatcgtta aaaacatgat gaacatgagg aagcgtcggg tgatcgatct
     1921 gtcgcagcgc gatgctattg aaggtcattt gcatgcgctc ttgcttattg agaccgctga
     1981 tctctttgag cgcaaacagt tgttgaccac tttgcgacgc gcgttgatct tccacgaggt
     2041 aaaccacacc ggaattgcct cgcccaatca agcttttgac caggtagcgt tcccgtacga
     2101 gtgcgccagg agcaagcatc tgttgcgcgt cttgcatgta tacctcactt aaatgtctca
     2161 ggtacgttat acgcttaata taaagaaaaa ctgcaattct gacattcttc tgatattgca
     2221 tatatcgaaa gttaccaatc tattagtaca acgtattaca tagacagaag tatctaatca
     2281 tctttaatat ctacatgcag ttcgttatta ttcgtgcaaa gggggatatt ctggcgatga
     2341 aacgtattag tgtacttggc gtgggctatt tgatgctcgg actgggcctg ttaggtattt
     2401 gcggtgccgc gctcctgggt ggaccgcgct ccactgcctc agcccagcct gcggcaaacc
     2461 catccttcgt gcgcgtcctg cacgcatcac catttgtggg aaccgctgat gtttttgtag
     2521 atggcgcaaa gctactcagc agctttgcct tcggcgctgt gaccggctat gcaacggtgc
     2581 cggcggggcc gcacaaagtc cagattgccc tggtggggaa gggcattggc gcggcggctc
     2641 tcacacaagt gttgacggtc agccccggct ttgcgtatac ggtagcggct attggcacaa
     2701 gcgcaagcaa tctatcgctg caagtcttcg tggacgataa cctgttgagt ccgggaacag
     2761 caaaattgcg tgtgtaccag ttgtcgcccg atggcggcac ggtgacggtc agcgcgggtg
     2821 ggaagacgct gctgagcggt attggttacc agaatgccag taactacgcg gtggttgcgg
     2881 cagggagcta tacctttaat gtcgactctc ccgcgaataa cgctacgctt acgacttcgg
     2941 cggcactcgc tgcgaacact gttgacagcc ttttcgtcgt cggcatgttc aatggcacgc
     3001 ccaaaagcga gttagtgtca tcgcaggccc agggattgcc cggtttgccc aacaccggca
     3061 gcgatccaaa cgcgatttcg cagataggtt ccgcgcagcc ctttgcgccg tcgccctggt
     3121 cctggccgtt gggcattgcc tcactgctca tcatcgggtg tggtgtctac ttgcgccgcg
     3181 tcaccgcgaa acgctagcat caggtaaggg tgcgtcgaat gtcccaggct cgtcgtctgc
     3241 tgctggcacg gttgctggta tgtctctctg cctctctctg cggactcgcg gcggtgctgt
     3301 tactgagcgc atgtcttagt aacagcacca ccgccagcgc aacggggcaa tccgtcgtgc
     3361 gcgccagcag cggcggtccg gcgccgaaag caagcgccac gccacatccc acgcccccca
     3421 cccgtcccac tgtctcgcct gacactccac tgcgcctgct catccctgcc atcggcgtca
     3481 acgccccggt ggagatgcta agctttggcg ctaacggcga cctggcaacg ccaactcagc
     3541 acccctggga tgatgtaggc tggtatgacc agggaccgca tccaggagaa cgcggcagcg
     3601 ccgtcatcga tggacatctt gatcgtcccg gcggcttccc tgcggtattc tggaacctgg
     3661 gccacgtgca agtaggcgcg gatgtgcagg tcacaaattc caccgggaaa acgctgcatt
     3721 tccgcgtaac gggcgtgatg tactacaccc ctgaccaggc cccgctccag gaaatattcg
     3781 gcaatcaaag cggcacctat ttgaacctta tcacctgcgc cggagactgg attcccagcc
     3841 agcaccagac caccct
//
LOCUS       DASZAK010001374         5202 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_140907, whole genome shotgun sequence.
ACCESSION   DASZAK010001374 DASZAK010000000
VERSION     DASZAK010001374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140907"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            346..1851
                     /locus_tag="VGT44_24115"
     CDS             346..1851
                     /locus_tag="VGT44_24115"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV2583962.1"
                     /translation="MQASKHGDLSTMPAANLKSGRVQTMYEENDLSGTNDGEEANDFN
                     EMEALLAAHTTPTNIKRGDVVDGVIVRIDQDEILVDIGLKSEGVLSSKEIPTSGEWSF
                     ENLHLNDKVLVYVIQPETSEGHALLSLKRANAERQWRIAEQQYRDNELLRARVIDYNK
                     GGLIVDVSGIRGFVPISQILNLKREEVAQGGDNQETAAKLQSMKDKELQLKIIEINRA
                     RNRLILSERLANQEYRQRRREELLDELKPGEVRRGVVSNLANFGAFVDLGGADGLVHI
                     SQLAWSRVNHPSEVLKVGQEVEVQVLSVDKEKKKIALSIKRAEVDPWTMVASRYTPGQ
                     IVNGTITKIAPFGAFARIEDGVEGLIHSSELTPGMDPRASLHEGQELQLRILRIEADR
                     RRLGLSLRQADELDTKEAETNTATSDTGSTHPSDDMISLEQVSSRADVQGEGQRQNKT
                     ERRDRGDKANDGTLLGGLAMHEESGETTAMAEAFRAAARQRSKEESEGSSE"
     gene            2093..4639
                     /locus_tag="VGT44_24120"
     CDS             2093..4639
                     /locus_tag="VGT44_24120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV2583963.1"
                     /translation="MNDRDRFDKFTERARKVLSLAQEEAQRFQHNYIGTEHLLLGLVR
                     EGEGVAAKVLSNLGVELNKVRSAVEFIIGRGDRIVLGEIGLTPRAKKVIELAVDEARR
                     LNHHYIGTEHLLLGLVREGEGIAAGVLESLGVNLEKVRTQTIQVLSQSGAPHERDAKH
                     SKTPTIDQMGMDLTNAARAGTLDPVIGRHQEIERVIQILSRRTKNNPALIGEPGVGKS
                     AIVDGLAQRLVAGEVPETLAGKRLLTLDVGSLVAGTKYRGEFEERLKKIIEEIRNSRD
                     CIIFIDEVHTLVGAGAAEGAVDAANILKPALARGELQCIGATTLDEYRKYIERDAALQ
                     RRFQEVIVREPTIEETLDILKGLRPRYEEHHRLTISDEALKAATEMASRYITDRFMPD
                     KAIDLIDEAASRVRMQNSLAPLNLKEAMKGLESVLREKEQAIQQQEYELAAELRDREV
                     KLRDRISRLESGWHRERGTERPIVGEEEIAQIVSMWTGIPVMRIAQEESQRLLQMEDG
                     LHSRVIAQNEAIEKIARAVRRARAGLKDPKRPIGSFIFMGPTGVGKTELAKALAEFMF
                     GSEEALIKIDMSEFMERHAAARLVGAPPGYVGYEEGGQLTEAVRRKSYSVILLDEIEK
                     AHPDVFNMLLQILEDGKLTDAKGRTVDFRNTIVIMTSNVGATLLNKEASIGFKQSKDK
                     AKANQNEYESMKGKVLGELKNTFKPEFLNRIDEVIVFHSLRIEEMYSIVELLLKRVRL
                     QLTEQQIELVVPQATKDYLIDKGFDPQYGARPLRRTIQRLVEDPLAEGLLEGKFHPGD
                     LVEAIVIDDDLKLVVRNRIEQLPPPSLPEPEPPEAALSAGGESISEASQG"
     gene            4746..5186
                     /locus_tag="VGT44_24125"
     CDS             4746..5186
                     /locus_tag="VGT44_24125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583964.1"
                     /translation="MPVILSYIQTRPLLEARKRGQTSVETSIDIGRSAISVALNTEGV
                     SFATGERLDWTSVEKISQSEVNCYTVSDNGTQPIQTFSKETNRLYSLMPTRGALSMLI
                     AGFVMHHIKEIDPWQHAQRMIATIAPMIGDGHEPGPPHGLSVRK"
BASE COUNT         1262 a         1460 c         1512 g          968 t
ORIGIN      
        1 agtgggggta tactgtgctc aggctcttat tatgtccgaa acacggctta cctgtttgca
       61 agcagttttc gtagaccgtt ttgagaacac aggagtgcgt tcttaccagt ccggtcgtaa
      121 gagaccgcag cagccgcgaa agcaatacct gcccaggcag cctcctccga taaaaaaaag
      181 cccaggtctc atgacgcatc ccccaggttc caggcatgca agtaattcgc ctccttgcgc
      241 aacacaacgg gacagagcga gagagagcaa gcgcatccgt caggaatacg ggctttttgt
      301 caaaaagacg ggctatcacc gtggatcgaa gggggtatta tacccatgca agcatctaag
      361 cacggcgacc tttcgacaat gccagcggca aatcttaaaa gcggacgggt acagacgatg
      421 tacgaagaaa acgacctatc agggaccaat gacggcgaag aagctaatga cttcaacgag
      481 atggaagcgt tgctcgcggc ccataccact cccaccaata tcaagcgcgg cgatgtagtc
      541 gatggcgtaa tcgtgcgtat tgaccaggac gagattctgg ttgacattgg tctcaaatcc
      601 gagggtgtcc tttccagcaa agagattccg acgagtggcg aatggtcctt tgaaaacctg
      661 cacctgaatg acaaggtact cgtgtatgtc attcaaccag agacctccga gggtcacgcc
      721 ctcctctctc tgaaacgcgc caatgcagaa cgccagtggc gcatcgccga gcagcagtat
      781 cgcgataacg agctgctcag agccagggtc atcgactaca acaagggcgg cctgatcgtc
      841 gatgtctccg gcatccgggg ctttgttccc atctcccaga ttctaaacct caagcgcgaa
      901 gaagtcgcgc agggaggcga taatcaggag acggcggcca agctgcagag catgaaagac
      961 aaagagctgc aactcaagat catcgagatc aaccgggcgc gcaatcgcct gatcctctcg
     1021 gagcgcctgg ccaaccagga ataccgccag cgccgccgcg aggaactgct cgacgaactc
     1081 aagccgggcg aagtgcggcg cggcgttgtc agcaaccttg ccaattttgg ggccttcgta
     1141 gacctgggag gcgcagacgg actggtgcat atcagccagc tcgcctggag ccgcgtcaac
     1201 catcccagcg aggtcctcaa ggtcggccag gaagttgaag ttcaggtctt gagtgtcgat
     1261 aaagagaaga agaagatcgc tctgtccatc aaacgggccg aggtcgatcc ctggacgatg
     1321 gtcgcgagtc gctacacccc aggccagatt gtcaacggca cgattaccaa aattgcgccg
     1381 ttcggggcct tcgcccgcat cgaagatggc gtcgaaggtc tgatccattc ctcagaattg
     1441 acaccaggga tggaccccag ggcaagcctg cacgagggtc aggaactgca attacgcatc
     1501 ctgcgtatcg aagccgaccg ccgccgtctg ggactcagcc tgcgacaggc cgatgagctt
     1561 gacacaaaag aggcggagac gaacactgcc acctcggaca ctggctctac tcacccctct
     1621 gatgatatga tatcgttaga gcaagtctct tcccgagccg acgtgcaagg ggagggccag
     1681 cgtcagaata aaactgagcg tcgcgatcgc ggtgataagg ccaatgacgg gactctgctc
     1741 ggcggcctgg ccatgcatga ggagagcggc gaaacaaccg cgatggctga ggcgttccgc
     1801 gccgcagctc gtcagcgttc aaaggaggag tcggaaggct catcagaata gccatttctc
     1861 gcgtctgaac ttgaacagtt gatcggtaga cctcttgctc gctttacttg ctatagtgcg
     1921 ggcaggaggt caattgtttc ataggacagt gacaattatg tgtaatagaa aggaagatgg
     1981 tattttcctt gacgatacaa aatcggtgtt gtaagatgaa tcatgctgtt tacctgagca
     2041 ttccagaggg cgcggttcca ctctctgcca ctgaaagggg ggtgataaac aggtgaacga
     2101 tcgagatagg tttgacaaat ttaccgagcg tgccaggaaa gtattgagcc tggcgcaaga
     2161 ggaagcgcag cgcttccagc acaactacat cggcacggaa cacttattgc ttggacttgt
     2221 acgtgaagga gagggggtag ccgccaaagt attgagcaat ctgggcgtcg aactcaacaa
     2281 ggttcgtagc gccgtggaat ttattattgg gcgaggagat cgcatcgtgc tgggcgaaat
     2341 tggcctgact ccccgcgcaa agaaggtgat cgagttggcc gtcgatgagg ctcgccgcct
     2401 gaatcatcat tatatcggta ccgagcatct gctgctgggc ctggtgcgcg agggtgaggg
     2461 catcgccgcc ggtgtgctgg aaagcctggg cgtcaacctg gagaaggttc gcacgcagac
     2521 gatccaggtg ctgagccagt ccggcgctcc tcacgaacgc gatgcgaagc actctaagac
     2581 accgaccatc gaccagatgg gcatggatct caccaatgcc gctcgcgcgg gtacgcttga
     2641 tcccgtgatc gggcgtcacc aggaaatcga gcgcgtcatc cagattctct cacgccgtac
     2701 caagaacaat cccgctttga tcggcgagcc gggcgtcggc aaaagcgcta tcgtcgatgg
     2761 gctggcgcag cgcctcgtcg ccggcgaagt cccggagaca ctggcgggta aacgcctgtt
     2821 gacgctcgat gtcggctccc tggtagctgg caccaagtat cgcggcgaat ttgaagagcg
     2881 gcttaaaaag atcatcgagg agatccgcaa cagccgagac tgcatcatct tcatcgacga
     2941 agtgcatacc ctggtcggcg cgggagcagc cgagggcgcg gttgatgccg cgaatatcct
     3001 caagcccgcc ctggcgcgcg gcgaactgca atgcatcggc gcaactacgc tcgacgagta
     3061 ccgcaagtat atcgagcgcg atgccgctct gcagcggcgt ttccaggaag tgatcgtgcg
     3121 cgagccgacc atcgaagaga cgctggatat cctcaagggc cttcgcccac gctacgagga
     3181 acaccatcgc ctgaccatca gcgacgaggc cctcaaggcc gcgaccgaaa tggccagccg
     3241 ctatatcacc gaccgcttca tgcccgacaa ggccattgac ctgatcgacg aagcggccag
     3301 ccgcgtgcgt atgcaaaact cgctcgcgcc cctcaacctc aaagaggcga tgaagggcct
     3361 ggagtctgtg ctgcgcgaaa aagaacaggc tatccaacaa caagagtatg agctggcggc
     3421 tgaactgcgc gaccgcgaag tcaagctgcg cgaccgtatt tccaggctgg aatccggttg
     3481 gcatcgcgaa cgcggcaccg agcgtcccat cgtcggcgaa gaggaaatcg cccagatcgt
     3541 ctcgatgtgg acgggtatcc cggtcatgcg tatcgcccag gaagagtcgc agcgtctgtt
     3601 acagatggag gatgggcttc actctcgcgt catcgcgcag aacgaggcca tcgagaagat
     3661 tgcccgcgcc gtgcgacgcg cccgcgccgg tctgaaagac cccaaacgcc cgattggctc
     3721 cttcatcttc atggggccga ccggcgttgg taaaacggaa ctggcgaagg cgctggccga
     3781 attcatgttc ggcagcgagg aagcgctgat caaaatcgac atgtcggagt ttatggaacg
     3841 ccacgcggct gcccgcctcg tcggcgcgcc tccgggctat gtcggctatg aagagggcgg
     3901 ccagttgacc gaggcggtac ggcgtaaatc gtacagcgtg atcctgctgg acgaaatcga
     3961 gaaggcgcac cccgatgtct tcaacatgct gctccagatt ctggaagacg gcaagctgac
     4021 cgacgcgaag ggtcgcaccg tggacttccg caatactatt gtgattatga cctccaacgt
     4081 aggcgcaact ctgctgaaca aggaagcctc gattggcttc aagcagtcga aagacaaggc
     4141 caaagccaat cagaacgagt atgaatcgat gaagggtaag gtcctcggtg agttgaagaa
     4201 cacctttaag ccggagttcc tcaaccgtat cgacgaggtc atcgtcttcc actcgctgcg
     4261 catcgaagag atgtactcga tcgtcgaact gctgctcaag cgcgtgcgct tgcaactgac
     4321 cgagcagcag atcgagctgg tcgtcccgca ggccaccaag gattacctga tcgacaaggg
     4381 cttcgatccg cagtacggtg cgcgcccgtt acgccgcacc atccagcgtc tcgtcgaaga
     4441 ccccctggcg gagggcttgc tcgaaggcaa gttccacccc ggcgatctcg tcgaagccat
     4501 cgtgatcgac gacgacctga agctcgtggt acgcaaccgc atcgagcagt tgccaccacc
     4561 cagcctgccc gaaccggagc cgcccgaagc agctctctcg gccggcggcg aaagcatcag
     4621 cgaggcgtcg caaggatagg ggatagtctg caataaaagg gagcgtaact gatgagagtc
     4681 tttacttttc atcagtcacg ctcccttttg ctgtaaagga agttctaact gaacctggat
     4741 catccatgcc tgtgattctc tcctacatcc agacgcggcc actgttggag gcgcgcaagc
     4801 gcggccagac cagtgtcgaa acctcaattg acattggcag aagcgccatc agcgtcgcat
     4861 tgaacactga aggcgtgagc tttgcaactg gcgaacgcct ggactggacc agcgtcgaaa
     4921 agatcagtca atccgaagtc aattgttaca ctgtaagcga caatggaacg cagcctattc
     4981 agaccttctc taaagaaacg aatcgcctgt acagtctgat gccgacgcga ggcgctctca
     5041 gtatgctgat cgctggtttt gtgatgcatc atatcaaaga gattgatccc tggcagcacg
     5101 cgcagcgtat gatcgcaact attgccccga tgataggaga tggacatgaa ccaggacctc
     5161 cacatggcct gtcggtgcga aaatgacgaa aagatacgac gt
//
LOCUS       DASZAK010001375         2153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_212165, whole genome shotgun sequence.
ACCESSION   DASZAK010001375 DASZAK010000000
VERSION     DASZAK010001375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212165"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1274
                     /locus_tag="VGT44_24130"
     CDS             111..1274
                     /locus_tag="VGT44_24130"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV2583965.1"
                     /translation="MPGSFRIGKIAGIDIDIHVSWIIILVLLTVSLATGWFPQLYPGW
                     SSATYWVVGLISALLLFVSVLLHELAHSLVARRRGLAVKSITLFIFGGVSNIEQEPKS
                     PGVEFQMAVVGPLTSLLIGAVCFLLQLPLRGTNSPLEGILFYLAVTNVLLGVFNLIPG
                     FPLDGGRVLRSIVWKITGSLAKATRAASITGQTIAYLFILLGIWLFFAGNILDGIWLG
                     FIGWFLLSAAQSANTQVMLQSALRGVTVGEVMNPTPPTVPANISLQKLIDEYFLPRGL
                     RFALVMQGDQLAGLITLSDIRHVPREQWEYIPVGQAMISVERLHVVSPQQSLNDVLPL
                     MAGRDVNQLPVVQNGIPVGIVSRDAIMRYLEVRRSLGSGTPKGDAGKQPSEAA"
     gene            1378..2025
                     /locus_tag="VGT44_24135"
     CDS             1378..2025
                     /locus_tag="VGT44_24135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583966.1"
                     /translation="MHHPASNDDEEVVVPSVAALVSILLTGLLYLVLPPNVIIGPNWL
                     LLVVEGVFVVPLVIDVLTAWDLSHTARRLLVMVPLGVSTLSLVAGVALFVLTLPADKH
                     ATNLLRAAALLWSFNILVFALWYWEVDGGGPWKRHRAGHRAADFLFPQQAAGERWVPH
                     FLDYLFVAFTGATALSPADTYPLTRLAKALMMVEAVLSLTVAGILIARAVNILGG"
BASE COUNT          398 a          635 c          601 g          519 t
ORIGIN      
        1 tcttcctcaa caggcagcgc ctgatccaga ggtggcagaa agagcgtaag gtcagttggc
       61 tgtggaagag acttgtacaa atcaatacag aggagagaag gagtcttaca atgcctggct
      121 cgtttcgtat cggcaagatt gccggaatcg acattgacat ccatgtgagc tggatcatca
      181 ttctcgtctt gctcaccgtg tcccttgcca ccggctggtt tccccagtta tatccggggt
      241 ggtcaagtgc cacctattgg gtagtaggcc tcatctcggc cctgcttctc ttcgtctccg
      301 tcctcttaca tgaactggcc cactcgctcg ttgcgcgtcg gcgcgggttg gccgtgaaaa
      361 gtatcaccct gttcatcttc ggcggtgtct cgaatatcga gcaggagcca aagagtccgg
      421 gcgtagagtt ccagatggcg gtggtcggcc cgctcaccag tctcctcatc ggcgccgtct
      481 gtttcctgct ccagttgccg ctgcgcggga ccaactcgcc gctggaaggc atcctgttct
      541 acctggcggt gaccaacgtc ctgttgggcg tctttaacct gattcctggc ttcccacttg
      601 atggcgggcg ggtgctgcgc tccatcgtct ggaagatcac cggcagcctg gcgaaagcca
      661 cccgcgcagc ctcaatcacc ggccagacca ttgcctacct cttcatcctg ctgggcatct
      721 ggctcttctt cgcgggcaac attctggatg gcatctggtt gggtttcatc ggatggttcc
      781 tgttgagcgc ggctcaatcg gccaacaccc aggtaatgtt gcagtccgcg ttgcgcggcg
      841 tcaccgtggg cgaggtcatg aatcccacac cgccgacggt ccctgccaac atctcactgc
      901 aaaaactgat cgacgaatac ttcctgcccc gcgggctacg tttcgccctg gtgatgcaag
      961 gcgatcagct ggctggcctg attaccctca gcgatatccg ccatgttcca cgcgagcagt
     1021 gggaatacat ccctgtcggc caggccatga tctctgtgga gcgcctgcac gtggtatccc
     1081 cgcagcaaag cctgaacgat gtattacctt taatggccgg tcgtgatgtg aaccagttgc
     1141 ccgtggtaca aaacggtatc ccggtcggta tcgtgagccg cgacgcgatc atgcgttatc
     1201 tggaggtgcg gcgtagtctc ggctcaggta ctcccaaagg cgatgctggg aaacagccgt
     1261 ccgaggcagc ctgatcaaga acgctctccc gcgtctccga tccgcgctgg cagacagcgc
     1321 agacgtttct tgatttcgta gcatacactg gaatgaatag tacactcgag aaaacagatg
     1381 catcatccgg cttcaaatga cgatgaggaa gtggtggttc caagtgtcgc cgcgctcgtc
     1441 agtatcctgc tgaccggcct gctctacctg gttcttccac caaatgtgat cattggtcct
     1501 aactggctcc tgctggtcgt agaaggggtg tttgtggtac cactcgtcat cgacgtcctg
     1561 acagcatggg acctctcaca tacggccagg cgtctgctcg tgatggtacc cctcggcgtg
     1621 agcacattat cactcgttgc cggggttgca ctcttcgttc tcacgttgcc agccgataaa
     1681 cacgcgacca atctattacg ggctgcggcc ctgctgtggt ccttcaatat cctggtcttc
     1741 gccttgtggt attgggaagt tgacggtgga ggcccctgga agcgccatcg cgctggacat
     1801 cgcgctgcag attttctgtt tccacaacaa gcagccgggg agcgttgggt gccgcatttt
     1861 cttgattatc tgttcgtcgc ctttaccggg gcaaccgcgc tgagtcctgc cgatacgtat
     1921 ccgctcacga gactggcgaa ggcgctcatg atggtcgaag cagtgctttc actcacagtc
     1981 gccgggatac tgatcgcccg cgcagtcaat atactcgggg ggtgaaccgg tcgtctatca
     2041 caacgcatcg gtgtgttttt tgtcgtcacg cgctttgtgt gagcaacgag gaggccaatt
     2101 cactacaccc gtttttacga cctggccaac gcttttcttg acgcctcgcg tgt
//
LOCUS       DASZAK010001376         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_141088, whole genome shotgun sequence.
ACCESSION   DASZAK010001376 DASZAK010000000
VERSION     DASZAK010001376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141088"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2005)
                     /gene="dnaE"
                     /locus_tag="VGT44_24140"
     CDS             complement(<1..>2005)
                     /gene="dnaE"
                     /locus_tag="VGT44_24140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV2583967.1"
                     /translation="IPLVLTNDLHYVDANDADSQDILLCIQTGKQLSDEKRMKFDSKQ
                     YYLKSPQEMALLFPDLPEALKNSVRIAEQCVVGLEFGQAKLPNYPIPPEFKTQNEYLY
                     HLCLQGVRERFGEMSEPIQRQLDYEFNLIAHKGFVPYFLIEWDFVNYARSRGIRCSAR
                     GSAAGSLVAYVLGITNVDPIRYHLFFERFLNPERKEMPDIDMDFPDDKREEVIAYVMQ
                     KYGPDRVAQMVTFNTMAAKASVKDAARVMGWQDIGDRITRLIPTGPKVTLKGSLETVR
                     ELTQLYEQSEQARKLIDQALELEGSVRTTGIHAAGVIIAHDSLDELIPLQLRDPKDSS
                     KGRVSQYEQKYLEQLGLLKYDFLGLSNLTILQNAVNFIRDARGEEINLDKIPLDDSKA
                     FELLSMGETTGVFQLEGAKMREYIKQLRPTRIEDLTAMVALYRPGPMDSIPDFIDAKH
                     GRKKVRYLDPRLKEWLEESYGVIVYQDQVLLITVHLAGFTWGEVDKFRKAMGKKIMAE
                     IEQYKDKFINGCIKNGVKPDVAEKLYELILPFGGYGFNKAHAASYAVVAYQTAYLKAN
                     YTAEFMAATLTTELSDAKKVANAIAECKRMGVQVLGPDVNKSDIGFTVEGGNVRFGLL
                     AIKGVGDGPAGEIVRVRKEGGAFRSLADLCTRVDPKQVTKGTLE"
BASE COUNT          407 a          559 c          548 g          491 t
ORIGIN      
        1 ctccaatgtg cccttcgtca cctgcttcgg atcgacgcgc gtgcagaggt ccgccagcga
       61 cctgaaagct cctccttctt tgcgtacacg cacaatctcc ccggccgggc catcgccgac
      121 acctttgata gcaagcaggc caaagcgtac attgccgccc tcgacggtga agccaatatc
      181 actcttgttc acgtctggtc cgagcacctg gacccccatg cgtttgcact ccgcgatggc
      241 attggcaacc ttcttagcat cagaaagctc agtagtaagg gtagctgcca taaactccgc
      301 cgtgtagttg gccttgaggt aagccgtttg ataggccacc actgcgtaac ttgcggcatg
      361 cgccttattg aacccataac cgccaaaggg caagatcagt tcgtagagct tctcagcaac
      421 gtcgggcttg acgccatttt tgatgcagcc gttgatgaac ttgtctttat actgctcgat
      481 ctcggccatg atcttcttgc ccatcgcctt gcggaacttg tccacctcgc cccaggtgaa
      541 accggccagg tggacggtga tcagcagcac ctggtcctgg tagacgatca cgccatagga
      601 ttcttccagc cattccttca ggcgtggatc caggtatctg accttcttgc gtccatgctt
      661 ggcatcgatg aagtcgggaa tactatccat tgggccaggg cgatagaggg cgaccatggc
      721 ggtcaggtct tcgatgcggg tcgggcgaag ttgcttgatg tattcacgca tcttagcccc
      781 ttcaagctgg aatactccgg ttgtctcacc cattgatagg agttcaaatg ccttgctatc
      841 atcgagtgga atcttgtcca ggttgatctc ttcgccgcgc gcatcacgaa taaaattgac
      901 ggcgttctgc aggatggtca ggttcgagag gcccaggaag tcatacttga gcaagcccag
      961 ttgctcgaga tatttctgct catactgtga gacgcgcccc ttgctggaat ccttcgggtc
     1021 gcgtagttgt agggggatca actcgtccag cgagtcatga gcgatgatca cacctgcggc
     1081 gtgaataccc gtcgtgcgca ccgacccctc tagctccaat gcctggtcga tgagtttcct
     1141 ggcctgctcg ctctgttcgt agagttgagt gagttcgcgc accgtctcga gcgaaccctt
     1201 gagtgtcacc ttgggacccg tagggataag gcgggtgatg cggtcgccaa tgtcctgcca
     1261 acccatcacg cgcgccgcat ctttgaccga tgctttggcc gccatggtgt tgaacgtgac
     1321 catctgcgct actctatcgg gtccatattt ctgcatgacg taggcgatca cttcttcgcg
     1381 tttgtcatcg ggaaagtcca tatctatatc gggcatttcc ttacgctcag ggttgaggaa
     1441 ccgctcgaag aacaggtggt agcggatggg gtcgacgttg gtgatgccca gcacgtaagc
     1501 aacgagactg cccgccgccg aaccgcgtgc agagcagcga atgccgcgag agcgtgcata
     1561 gttcacaaag tcccactcga tgaggaaata gggcacgaat cctttgtgcg cgatgaggtt
     1621 gaactcgtag tcgagttgcc tttgtatcgg ttcgctcatc tcaccgaagc gttcgcgtac
     1681 tccctgcaaa cacaggtgat agagatactc gttctgtgtc ttaaattccg gtggaatggg
     1741 gtaattcggc agttttgcct ggccaaattc cagcccgacc acacattgct cggcgatacg
     1801 cacgctgttc ttcagcgcct ccggcaggtc ggggaagagg agtgccattt cttgtgggct
     1861 cttgagatag tattgcttgc tgtcgaactt catgcgcttc tcatcagaga gttgcttgcc
     1921 cgtctgaata cacagcagta tgtcctgcga gtcggcatcg ttggcatcga cataatgcag
     1981 gtcattggtg aggaccagcg gaata
//
LOCUS       DASZAK010001377         1815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_212296, whole genome shotgun sequence.
ACCESSION   DASZAK010001377 DASZAK010000000
VERSION     DASZAK010001377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212296"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="VGT44_24145"
     CDS             <1..1049
                     /locus_tag="VGT44_24145"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV2583968.1"
                     /translation="PGSKPLLFALIQLLEGDVLLPAPSWVSYAPHAHLANRRVALVET
                     DPRDHHRLTTGALADALTRAQHAGADPRILVVNTPSNPTGGMFAREDIEAIASWAREH
                     GITIISDEIYAELAHGWREHLSPARYYPEGCIVTGGLSKAFSAGGWRLGYAALPPGDA
                     GQRLMSSLQALASEIWSAASTPVMQAAVTAYSANPDLETYVQSCARLHSYTTSRLYEV
                     LVDLGVDCPRPAGGFYLYPDFAPWQASLRQRGITTSNQLARHLLDEWNIATLPGTAFG
                     DKPEALRLRLATSMLYAQAPALSAEDQEQALWHMIEQAGTWTNEDGMDQRVRLELPML
                     ERARKRLGEFVESL"
     gene            1280..>1815
                     /locus_tag="VGT44_24150"
     CDS             1280..>1815
                     /locus_tag="VGT44_24150"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEV2583969.1"
                     /translation="MPGAIEIFCCYAREDQGLMKALRKHLMPLQRRNLVTIWSDIEID
                     AGDAWETEVQKHLESAHIILLLISSDFMASDYCYSTEMERALERHAKGEARVLPIILR
                     PTAWKGAPFDRLQVTPTNAKPVTDSRSWISIDEALNDVIEHITPLVFELLLPLYIDEA
                     MQLVKRGMHLEALALYERA"
BASE COUNT          405 a          600 c          479 g          331 t
ORIGIN      
        1 cccctggcag caagccgctg ctattcgcgc tgatacaact gctggaaggc gacgtgctgc
       61 tgcccgcgcc ctcctgggtc agctacgccc cgcacgcgca ccttgcaaac cgccgcgtcg
      121 cactcgttga aaccgacccg cgcgaccatc accgcctcac aaccggcgcg ctggccgatg
      181 ccctgacccg cgcgcaacat gcaggggccg acccacgcat cctggtcgtc aatacgccga
      241 gcaacccgac cggcggcatg tttgcccgcg aggatatcga ggccatcgca agctgggcgc
      301 gcgaacacgg catcaccatc atcagcgacg aaatctacgc tgagctggcg cacggctggc
      361 gcgagcatct ctcacccgcg cgctattacc cggagggctg catcgtgacc ggcggactct
      421 cgaaagcctt ctccgccgga ggctggcggc tcggctacgc agcccttcca ccgggcgacg
      481 cgggccaacg gctgatgtct tccctgcaag cgctcgccag cgaaatctgg tccgccgcct
      541 ccacaccggt aatgcaagcc gccgttaccg cctacagcgc caatcccgac ctggagacct
      601 acgtccaatc ctgcgcgcgc ctgcacagct acaccacctc tcggctatac gaagtattgg
      661 tcgatctcgg cgtcgattgc ccgcgacccg ccggaggctt ctacctctac ccggactttg
      721 ccccctggca ggcatccctt cggcagcgcg gcatcaccac ctccaatcaa cttgcccgcc
      781 acctcctcga cgaatggaat atcgccacac tccccggcac agccttcggc gacaaacctg
      841 aagccctgcg cctgcgactc gcgaccagca tgttatatgc acaagcgccg gcactatccg
      901 ccgaagatca agagcaagcc ctgtggcata tgatagagca ggccggaaca tggacgaatg
      961 aggatggaat ggatcagcga gtaaggctgg aattgccgat gctggaacga gcgcggaaga
     1021 ggctagggga gtttgtagaa tcgctataac cggagcagtt acaaatctca gcatggcgac
     1081 cacaagggat cgccactacg ataacgtggc ttcgcgggtt gctttatcgt agtggcgatc
     1141 ccttgcggtc gccatgccac aactcatata tcaacttggg gaataactaa tggaatattg
     1201 tcactttacc tgaactatca tatactacag tcaccaaacg caaatcgaag cgatctggaa
     1261 ggagtctgag tagttgtcta tgccaggcgc catcgaaatc ttctgctgtt acgcccgcga
     1321 agatcagggg ctgatgaaag cgctccgcaa gcacctcatg cctcttcaac ggcgcaacct
     1381 cgtcacgatc tggagcgata tcgagatcga tgcgggcgac gcctgggaaa ccgaagtgca
     1441 aaaacacctg gaatcggcgc acatcattct cttactaatc agctccgatt tcatggcctc
     1501 cgattactgt tacagcaccg agatggaacg cgccttagag cgccatgcca aaggagaggc
     1561 gcgcgtcctc cccattattt tacgccccac tgcctggaaa ggcgcgccct tcgacaggct
     1621 ccaggtcact ccaaccaacg ccaaaccggt cacggatagt cgcagttgga tcagcatcga
     1681 cgaagcgttg aatgatgtca tcgaacacat taccccgctt gtttttgaat tgctgctgcc
     1741 gctctacatc gacgaagcca tgcaactcgt caaaaggggc atgcatctcg aagctctggc
     1801 gttgtacgaa cgcgc
//
LOCUS       DASZAK010001378         1639 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_141289, whole genome shotgun sequence.
ACCESSION   DASZAK010001378 DASZAK010000000
VERSION     DASZAK010001378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141289"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1639)
                     /locus_tag="VGT44_24155"
     CDS             complement(<1..>1639)
                     /locus_tag="VGT44_24155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007912019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV2583970.1"
                     /translation="ENTVVLPHPQVSAHHARLVREGGTYRIFDLHSTNHVYLNAQLTT
                     DALLKMGDEIRIGPYRLIFESTQLKQYDESNSIRIDALNLKKYGNNHTTLLNNISLSI
                     APRTFIAVVGGSGAGKSMLVDALSGLRPAHEGKVLYNGQDYYRNLAAFTTQLGYVPQD
                     DIVHRDLTVERALYYAAKMRLPGDFTEGQIRQRITEVLEDVEMMGRQKLLIRKLSGGQ
                     RKRVSIALELLANPSLFFLDEPTSGLDPGLDRKMMFLLRKLADKGHTIILVTHATNNI
                     STCDYVCFLAQGGRLAYFGPPDEAKAYFGKGDFAEIYGSLESTEDNNTILEDAEARFR
                     GSKDYQMYVAQPLKRVQATTGQASTGDSIASKRERRRPKRGNPWKQFMLLCMRHGELL
                     KNNVSNLAILLLQAPLVALLLILLVRFEIGAGIFDPNHLVQCRTQILTSSGQLALPGV
                     ANVADLVNCQQVVNFLNTNPTGVQYAQVRGSTNQALQDFIVPAQSAEAQLVIFLVAFF
                     AVLFGCINGTREIVKEAAIYQRERTVSLGIRPYLFSKI"
BASE COUNT          359 a          434 c          473 g          373 t
ORIGIN      
        1 tgattttaga gaacaggtag ggacgaattc ccagggagac ggtgcgttct cgttgataaa
       61 tggcggcttc tttcacgatc tcgcgtgtgc cgttaatgca gccaaagagg acggcgaaaa
      121 aagcgacgag gaagatgacg agctgagcct cagcgctctg ggccggaact atgaaatcct
      181 gcaacgcctg gtttgtgctg cccctcacct gcgcatactg cacaccggtg ggattggtat
      241 tgaggaagtt gacgacttgc tggcaattga ccaggtctgc cacatttgca acgcctggaa
      301 gcgcaagttg gccggaggat gtgaggatct gcgttctaca ctggacaaga tggttcgggt
      361 caaagatgcc cgcgccgatc tcgaagcgaa ccagcaggat gagcagcagg gcgacgagcg
      421 gagcctgcaa caggagaatg gcgaggttac tgacattgtt cttgagcagc tctccatgac
      481 gcatgcagag cagcataaac tgcttccagg gattgccccg tttcgggcgt cttctctccc
      541 gcttactagc gatgctatcg cccgtagatg cctggccggt cgtagcctgt acccttttga
      601 gaggctgagc cacgtacatc tggtaatcct tcgagcctcg aaaacgagcc tctgcatctt
      661 ccagtatcgt attattgtcc tcggttgact ccaggctgcc atagatctcg gcaaaatcgc
      721 ctttgccaaa ataagccttt gcttcatcag gaggtccaaa gtaagcaagg cgtcctcctt
      781 gtgccagaaa acaaacatag tcacaggtgc tgatgttgtt cgtcgcgtgg gtcacgagga
      841 tgatggtatg tcccttgtca gccaattttc tcaggagaaa catcatttta cggtcaaggc
      901 caggatcaag gcccgacgta ggctcgtcca ggaagaagag actgggattg gccagcagtt
      961 ccagagcgat agagacgcgc ttgcgctggc caccggagag ttttctaatg agcagcttct
     1021 gcctccccat catttccacg tcctctagca cttcagttat acgctgccgg atctgtccct
     1081 ccgtgaagtc gccgggcagg cgcattttgg cggcgtagta gagcgcccgc tcgaccgtta
     1141 aatcccggtg gacgatatca tcctgcggga cgtagccaag ctgcgtcgta aacgcggcaa
     1201 gattgcggta gtaatcctgg ccattataaa gtactttccc ctcgtgcgcg ggccgcagcc
     1261 cgctcagagc atcgaccagc atcgatttac cggcgccaga ccctcccacg acggcgataa
     1321 atgtccgggg agcaatcgag agcgagatat tgttgagcag cgtcgtgtgg ttgttcccgt
     1381 atttcttgag gttgagcgca tcgatgcgga tggagttact ctcatcatac tgtttcagtt
     1441 gcgtgctctc gaagatgagc cggtaggggc cgatgcgtat ctcatcgccc attttgagca
     1501 gcgcatcggt ggtgagctgc gcgttcaggt acacatgatt cgtgctgtgg agatcaaaga
     1561 tgcgataggt cccgccttcg cgtaccaggc gcgcatggtg cgcagaaacc tggggatgag
     1621 gcagcaccac cgtattctc
//
LOCUS       DASZAK010001379         5323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_212469, whole genome shotgun sequence.
ACCESSION   DASZAK010001379 DASZAK010000000
VERSION     DASZAK010001379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5323
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212469"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VGT44_24160"
     CDS             <1..1214
                     /locus_tag="VGT44_24160"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEV2583971.1"
                     /translation="QTEQQSAGSLLRRNRDVAVLSAIAGHLNRKIDVHEALQEVLLQV
                     TRLLGLRTGWVWLLNERDEPYLAAVQSLPPYLGEHRERMTGSCQCLDTFLQGDLEGAK
                     NIDVLRCSRLKNAERDKDPSSLGLRFHASIPIYAGITPIGVLNVASEDWRELQEEELQ
                     LLHIIGDQMGLAIQRARLSAEHTRAATRLATIEERNRLAREIHDTLAQGLTAITLQLE
                     TADALAQTRPDRAQEAIRRALALARSNLEETRRSMIDLRAIPLQNRSLPDALVALTQE
                     TAQEGIEVECHCVLPHDYPLLLQRVETALYRIAQEALSNAYQHAGASHIAVRLEIIDG
                     ELCLTIQDDGRGFEPDVVIAAEQTSGPAAGHFGLTGINERVKLLGGVVCIQSEPGAGT
                     CLSVCIPMNDE"
     gene            1237..1980
                     /locus_tag="VGT44_24165"
     CDS             1237..1980
                     /locus_tag="VGT44_24165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV2583972.1"
                     /translation="MTIRILLADDHPIVREGLRAVLEIQADFEVVGAPDQAASGEEAL
                     RLAIELQPDILLLDLEMPGMDGVETIRRLRAQARQSSANSTGAARHAAHDATQRPRII
                     VFTAFDNDERIIAALEAGANGYLLKGAPRDEIFNAIRVTMQGGSLLQPVIASKLLRHM
                     GQHPLSPSAHSSASASQHPYEALTERELEVLSLLAQGMPNKEIAARLIISERTAKFHV
                     SSIMGKLGATNRTEAVSLAAQRGLVRLQS"
     gene            2134..2493
                     /locus_tag="VGT44_24170"
     CDS             2134..2493
                     /locus_tag="VGT44_24170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583973.1"
                     /translation="MEDVSYAHKLSIMPRVAATLVVVALISGIALACIGVVAMLGPTL
                     AHGDTVEHEAGTIVDIGPGKDFVLLTAQGQRLTFLCEAQCRASLGHMQRHMSEHAHTD
                     VYYLPGPEKSLMALDVD"
     gene            2499..4460
                     /locus_tag="VGT44_24175"
     CDS             2499..4460
                     /locus_tag="VGT44_24175"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV2583974.1"
                     /translation="MTATSGLPENTIKRVERGDIVIVGNGIAGLTAAVEARRLAPDRD
                     IVVITDQSHPTINTPALKQYAIGKLEREQLLAYPAGTERAQLIHVINARVEEIHAQEK
                     YITMRGGRGLGYDALLIATGSGPTGLPANLPGRDFDGVLTLHRLPDYLDLRRRLSDVN
                     EAVVIGGGAHAIETVMGLLYWGVRVHWLIRGETFLPKMLDQPASTMVLESIQRAGVMV
                     YTGTEAAGIVGRVGAVAGVVTNRQQMLPCQLVLVCTGTSPISSLARHCDVPIQHKQGI
                     TVDDHLRTSARDIYAAGDVAAIQDPQTGQFAPRAQWYSAVQQGRIAAAAITDSPLNES
                     FGVPWHATRLGELSMLTVGNVLQSNQAASTLTDNSRGSYRRMCVLDDRLVGYLSLGAV
                     QPDSLAIKRIIDEGLSIRDIKKELLRGTFDARKYFSRKRTYAAQHMVTTGKLPRDLEP
                     RPLTTRKLEAPPIAARAVSPASPARPAAFEQAATFAQGIPLARSLPSTGPLATPASVA
                     ANQARHTHDTGEDVQLFAIPFVPVAPDTIAVERQPVAERTRYSGQRGSRTAPVVESTL
                     VRPPVSLTSPMSPAAPESPSVSTEPLMTPAGRGLKRQFKAPPTAKPKKRQSRQFSKLL
                     ERDSDAPEHYPSSSLWAYSDKVPVVKKGR"
     gene            4461..4778
                     /locus_tag="VGT44_24180"
     CDS             4461..4778
                     /locus_tag="VGT44_24180"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEV2583975.1"
                     /translation="MLRRMVPGRAGVMVHKTASQVAQELQACIGCNECLLACPALSEP
                     VTIDVLNRETFSGPISAPAARFARACYQCGACVAPCPVGLHRDAMMMWLKVRLLRSDW
                     DGK"
     gene            4799..>5323
                     /locus_tag="VGT44_24185"
     CDS             4799..>5323
                     /locus_tag="VGT44_24185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV2583976.1"
                     /translation="MGKFLRVIVTLLLLSGGIAFYAWYSRTSQDASPDSVAGYALAIL
                     GTLFIIMAGVLFSRRRRIHKNRVVGQLHASLQWHMIFAIVGLEFLFLHSFGNFNPRTG
                     TYALYGMIALAISGVIGRLLDRLLPRLIAREAHKVLTATGEDRVESISQKLQAIVLHN
                     TTTIQGFAPPNAARS"
BASE COUNT         1067 a         1650 c         1663 g          943 t
ORIGIN      
        1 aacaaaccga gcagcaaagc gccggttcgc tgttgcgcag gaaccgcgac gtggcggtgc
       61 tttcggctat cgcggggcat ttgaaccgca agattgacgt gcatgaggcg ctgcaagagg
      121 tgctgttgca ggtcacgcgg ctgctggggc tgcgcacggg ctgggtctgg ctgctcaacg
      181 agcgcgacga gccgtatctg gcggcggtgc agtcgctgcc accctatctg ggcgaacacc
      241 gcgagcgtat gacgggcagt tgtcagtgcc tcgatacctt cttacagggc gatcttgagg
      301 gggcaaagaa tatcgatgtg ctgcgctgca gccgtttgaa aaatgccgag cgcgacaagg
      361 acccatcctc gctgggactg cgcttccacg ccagcatccc catttacgcg ggcataacgc
      421 ccatcggggt actgaacgtg gccagcgagg attggcgcga actgcaagaa gaagaactgc
      481 aactgctgca catcatcggc gaccagatgg ggctggccat tcagcgcgcg cggctctcgg
      541 ctgagcatac acgcgccgcc acgcgcctgg cgaccatcga ggaacgcaac cgcctggcgc
      601 gcgagattca tgatacgctg gcgcaagggc tgaccgccat cacgctgcaa ctcgaaacgg
      661 cggacgcgct tgcccagacg cgccccgacc gcgcgcagga ggccatccgg cgcgccctgg
      721 ccctggcgcg ttccaacctc gaagagacgc gccgctcgat gatcgacctg cgcgccatcc
      781 ctttgcagaa tcgctcgctg ccggatgcgc tcgtagcgct gacgcaggag acggcgcagg
      841 aaggcatcga ggttgaatgc cactgcgtgc taccccacga ctacccgctt ctgctgcagc
      901 gcgtcgagac cgccctgtac cgtatcgcgc aggaggcgct ctcgaacgct taccaacacg
      961 cgggagccag ccatatcgcc gtgcgcctgg agatcatcga tggcgaactg tgcctcacca
     1021 ttcaggacga tgggcgtggg ttcgagccag acgtggtgat cgcggcggaa cagacatccg
     1081 gccctgccgc agggcatttc gggctgaccg gcattaacga gcgcgtaaag ctgctcggcg
     1141 gggtcgtgtg catccagagc gagccgggcg cgggtacctg cctatccgtc tgcatcccta
     1201 tgaacgatga atgaaagcgt gaggtagcga aggcgcatga cgatccgtat attgctggcc
     1261 gacgatcatc ccatcgtgcg cgaaggattg cgagccgtgc tggagataca ggcagacttt
     1321 gaggtggtcg gcgcgcccga ccaggccgcg tcgggtgaag aagcgctgcg tctggcgatc
     1381 gagctacagc cagatatcct gctgctcgac ctcgaaatgc cgggcatgga cggcgttgag
     1441 acgattcgcc gcctgcgtgc acaggcgcgg cagagtagcg cgaacagcac aggcgcggcg
     1501 cgccatgccg cacacgacgc gacgcaacgc ccgcgcatca tcgtcttcac cgccttcgac
     1561 aacgacgaac gcatcatcgc cgccctggaa gcaggagcga atggctacct gctcaaaggc
     1621 gcgccccgcg atgaaatctt caacgccatt cgcgtcacga tgcagggcgg ctcgctgctc
     1681 cagcccgtca tcgcctcgaa gttgctgcgt catatggggc agcatcccct ctcaccctcc
     1741 gcgcactcga gcgcctccgc gtcgcagcat ccctatgaag cgctgaccga gcgcgaactt
     1801 gaggtgctgt ctctgctggc gcaggggatg ccgaataaag agattgccgc gcgcctcatt
     1861 atcagcgagc gcacggccaa gttccacgtc agttccatca tgggcaagct cggcgcgacc
     1921 aaccgcaccg aggccgtttc cctggctgcg cagcgcggat tggtgcggct gcaatcatag
     1981 tcagtacagg tgtgattatc tccaccggaa gtgggatatt ctggctaata tcctgctcat
     2041 ccttgcttga agcgatcact atcgatatac aatgctaaaa gcatcaagat gtttcaaggt
     2101 gtttcagagg tatcaaacta gtgcggagac aaggtggaag acgtttcata cgcgcataaa
     2161 ttatcgatca tgcctcgtgt agccgccacg ctcgtcgtgg tggcgttgat cagcggcatc
     2221 gcgctggcct gcatcggcgt cgtagccatg ctcggtccaa cgctggcgca cggcgatact
     2281 gtcgaacacg aggcaggcac gattgtcgac atcgggccgg ggaaggactt cgtcctgctg
     2341 acggcgcaag ggcagcgttt gacgtttctg tgcgaggcgc agtgccgcgc ctcgctgggg
     2401 catatgcagc gccacatgag cgaacatgcc cataccgatg tctattacct gcccgggccg
     2461 gaaaagagcc tgatggcgct ggatgtggat tgacagcatt gactgcaaca agcggactac
     2521 cagaaaatac aataaagcgg gttgagcgcg gggacattgt gatcgtcggc aatggcatcg
     2581 cgggtctgac ggctgccgtc gaagcgcggc ggctcgcgcc cgaccgcgat atcgtcgtca
     2641 tcacggatca gagccatccc accatcaata cgcccgcgct gaaacaatac gcgattggca
     2701 agcttgagcg cgagcagtta ctggcgtatc cggcaggcac cgagcgcgcc caactgatcc
     2761 acgtcatcaa cgcgcgcgtc gaagagattc acgcgcagga gaaatacatt acgatgcgcg
     2821 gcgggcgcgg cctcggctac gacgcgctgc tgatcgccac aggcagcggg ccgacaggat
     2881 tgcccgcgaa cctgccggga cgcgactttg atggcgtact gacgctgcat cgcctgcccg
     2941 attatcttga cctgcgccgg cgactaagcg atgtgaacga ggcggtggtg atcggcggcg
     3001 gcgcgcacgc catcgagacg gtgatgggtc tgctttattg gggcgttcgc gttcactggc
     3061 tgattcgcgg cgaaacgttc ctgccgaaaa tgctcgatca gccagcctcg acgatggtac
     3121 tggagtcgat tcagcgcgcc ggagtgatgg tttatactgg cactgaggcg gcgggtatcg
     3181 tcggcagggt gggcgcggtg gccggagtgg tgacgaaccg ccagcaaatg ctgccgtgcc
     3241 agcttgtgct ggtctgcacc ggcacatcgc cgatctcatc gctggcacgc cactgcgacg
     3301 tgccgataca gcacaagcag ggcatcactg tggacgatca cttgcgcacg agcgcgcgag
     3361 acatctacgc ggcaggcgac gtggccgcta tccaggaccc acagacgggc cagttcgctc
     3421 ccagggcgca gtggtactcg gcagtgcagc agggacgtat cgcggcggcg gccatcacag
     3481 actcgccgct gaacgagagc ttcggcgttc cctggcacgc gacacggttg ggcgagcttt
     3541 cgatgctgac agtcggcaac gtgctgcaaa gcaatcaggc agcctccacc ctgacggata
     3601 acagccgggg cagctaccgg cgcatgtgcg ttctcgacga ccggctggtg ggctatctct
     3661 cgctcggcgc tgtgcaacct gatagcctcg ccatcaagcg tatcatcgac gaggggcttt
     3721 cgatccgcga tatcaaaaag gagctgctgc gcggtacctt cgacgcgcgc aagtacttct
     3781 cacgcaagcg cacctacgca gcgcagcata tggtgacaac cggaaagctc ccgcgtgacc
     3841 tggagccacg tcctctgacg acgaggaagt tggaagcgcc tcccatcgcg gcgcgcgctg
     3901 tatcgcccgc gtcaccggcc aggccagccg cgtttgaaca ggctgccaca ttcgctcagg
     3961 gaatacccct ggcgcgctcg ctgccatcga ccggcccgct cgccacacct gccagcgtcg
     4021 cggccaatca agcgcgccac acgcacgata caggcgaaga cgtgcagttg ttcgctatcc
     4081 cattcgtacc tgtcgcgccc gatacgattg cggtagagcg gcaacctgtc gcggaacgta
     4141 caaggtattc cggtcaaaga gggtcaagaa cagcgcctgt tgttgagtca acgctggtca
     4201 gaccgccggt atcactaacg tcgcccatgt cgcctgccgc gcctgaatcg ccatcagtat
     4261 caacagagcc actgatgacc ccggcagggc gcggactaaa acgacaattc aaggctccgc
     4321 ccacggcaaa gccaaagaaa cggcaaagcc gacagttcag caagctgctc gagagagaca
     4381 gcgacgcgcc ggaacactat ccttccagca gcctgtgggc gtatagcgac aaggtgccgg
     4441 tggtgaagaa aggccgataa atgttgagaa gaatggtgcc aggacgggcc ggggtgatgg
     4501 tgcataaaac ggcctcgcag gtcgcgcagg agcttcaggc gtgtattggc tgcaacgaat
     4561 gcctgctcgc ctgtcccgcc ctgtccgaac ctgttaccat cgatgtcctc aaccgcgaga
     4621 ccttctccgg gccgatctcc gcgcccgccg cgcgtttcgc gcgcgcgtgt taccagtgcg
     4681 gcgcgtgcgt cgctccctgc ccggtagggc tgcaccgtga cgcgatgatg atgtggctga
     4741 aagtgcgcct gctgcgctct gattgggatg gaaaatgaga gggagcaaca ggtaaaacat
     4801 gggcaaattt ctacgtgtga tcgtcacact gctgctgctc tccggcggca tcgcgttcta
     4861 cgcctggtac tcgcgcacca gccaggatgc ctcgcccgac agcgtggccg gttacgcgct
     4921 ggcgatcctt ggcacgctct tcatcattat ggcaggtgta ctatttagca ggcggcggcg
     4981 cattcacaaa aaccgcgtcg tcggccagtt acacgcctcg ctgcaatggc atatgatctt
     5041 cgccatcgtc ggcctggaat tcctgttcct gcactcgttc ggcaatttca acccgcgcac
     5101 cggaacctac gcgctttatg gcatgatcgc gctggccatc agtggggtaa ttggtcgcct
     5161 gctggatcgc ctgctgccgc gcctgatcgc cagggaagcg cacaaggtgt tgacggcaac
     5221 gggggaagat cgcgtcgagt cgatatcgca gaagctacag gcgattgtac tgcacaacac
     5281 gaccacgatt cagggctttg ctcctccaaa cgcggcccgc tcg
//
LOCUS       DASZAK010001380         5932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_212595, whole genome shotgun sequence.
ACCESSION   DASZAK010001380 DASZAK010000000
VERSION     DASZAK010001380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212595"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VGT44_24190"
     CDS             complement(<1..513)
                     /locus_tag="VGT44_24190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fuconate dehydratase"
                     /protein_id="HEV2583977.1"
                     /translation="MPITITGFMAYDIRFPTSRTLAGSDAMNNDPDYSAAYVVLQTDH
                     PANLEGHGLSFTIGRGNEVCVAAIWALEPLVIGRSLESFTSDMRAFWRHITGDSQLRW
                     IGPEKGAIHMATAAVVNAVWDLWAKVEGKPVWKLLSDMTPEQLVSCIDFRYISDALTP
                     DEALDLLCRHA"
     gene            complement(564..1337)
                     /locus_tag="VGT44_24195"
     CDS             complement(564..1337)
                     /locus_tag="VGT44_24195"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV2583978.1"
                     /translation="MKTKPPVEPGEVGVDSNAQVIDEDFSDEVFELLWGHGRNAIPTA
                     VDYAYELIWKQLVFSNQRKEERLSDVALAEKLGVSRTPVRQALERLVQDGLVRSDPRR
                     GFWVRLFTAQDIQEIYELRGALEVLALRLAAPRLDPADLRSQLDLLYKVREQLDQRPI
                     SLFLQCDFRLHTLLIRASGNSRLIHYLSTLRSQFSVFQIKDTLYPQRMEAALDDHEQI
                     LVALLAGNIEKAAELLAAHISHAMANVLADIFEENGTAL"
     gene            1543..2631
                     /locus_tag="VGT44_24200"
     CDS             1543..2631
                     /locus_tag="VGT44_24200"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV2583979.1"
                     /translation="MNSKFVSRLRALSALVVIATVLVACGGTSGTTSTSGTSTTSGGP
                     LASGASFKLGVSLTFNNTDFWTNYISYEQKFASQYNATLIGPLVANNDAAKQITDIHT
                     LIQEGAQALIVNPVDSAAIAPALDYAASKNVPVVSVDVAPSSGKVFMIVRADNVLYGQ
                     NSCKFIASHATGTSGHIAILEGDLASLNGKDRTDGCNQVIQQNYPNFKTVEYATKWDT
                     PTAVNDAKTALSTYPDLRGIYVEWGAPEDGILAAEQAAGKFTPVGSSSHIVLVGNDGV
                     PHEHALIRSSKLDATISQPANSYAQYAVFYARAALEGKTYAAGQTTDHNSTYVNLQGN
                     LEDALPAPVVDSTNVNDPNLWGNSKSGS"
     gene            2683..4272
                     /locus_tag="VGT44_24205"
     CDS             2683..4272
                     /locus_tag="VGT44_24205"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEV2583980.1"
                     /translation="MLEPVNGGPTGARLSVGVAQSVWKAFGETQALRDVSLEVEAGEC
                     HGLVGRNGAGKSTLVAIFTGLIRPDRGVVRLGAEAAPSLADRAAWQRLVACVYQKSMV
                     VPTLTVAENIFLNRATGDDHGIVNWRAVRSRARQVMLDWGFDLDVNRLARDLTVEQRQ
                     VVEIARALSVGARFLILDEPTASLEKAAIERLFERIRRLKESAVGILYISHHLEEIYE
                     VCDRVTVLRDGERVLSTPVSEIEQSRLVEAMVGVAPQRVTEIVAPLRVIASTRPRLEI
                     SHLNVPATLGPVQDVSFSVSSGECVGLVGLQGSGTATVADAVVGLVKPLSGEIKLDGK
                     PIRTGKVDATFRQGVTYVPEDRHARGFVPYLGVGENLTLYILDRLSHWGIVSRSGRDR
                     VALDLVNQLGIVTSGLNQQVGQLSGGNQQKVVVGRALAQHPAVLVAVAPTVGVDVAAK
                     ESLLNVIGEARDGGTAVLLVSDDLEDLRICTRLLVMVKSRLVKEYRQPPWDRHELIAV
                     VEGLDPASIRGNNGMSKGDSE"
     gene            4269..5306
                     /locus_tag="VGT44_24210"
     CDS             4269..5306
                     /locus_tag="VGT44_24210"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV2583981.1"
                     /translation="MSKSTMDRPVDRAGQTLAGTLRRQGRHLIANWIRELALLPVIVL
                     LLVVGSFVNPAFFTSDNLINVAQQSAALGAVVVAETLILLTGKFDLSLQATYGLAPMV
                     GAWLIVSKDGQGLGTNWNPFIGIAIVLLVGLVVGAFNGFMVIKLGFNAFIFTLAVLIL
                     LTGIQVGIVSGRTIYHLPDAYIYLGSQYLLGVPVSVWVTGAIFLLSALFLRYHRIGRA
                     IYAIGGNTEAARAAGIHVDRVRIGVFIVASILAALAGLMTAGQVVAVTASQGNNLIFQ
                     VFAAAVIGGISLEGGRGRMLGALTGVILLALVTNILILSQIQTFWIDAANGLIILIAL
                     GLARLIGSERS"
     gene            complement(5318..5689)
                     /locus_tag="VGT44_24215"
     CDS             complement(5318..5689)
                     /locus_tag="VGT44_24215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase superfamily protein"
                     /protein_id="HEV2583982.1"
                     /translation="MQMKLELVAVPVSDVDRAKAFYTEKIGFNADHDYQVRDDLRFVQ
                     LTPPGSACSIVIGVGITDMQPGSVKGLQMVVPDVKEVRDELVKRGVEASEIEVMPWGS
                     FVFFSDPDGNGWSLQQLPVRA"
BASE COUNT         1272 a         1665 c         1675 g         1320 t
ORIGIN      
        1 agcatggcgg catagcaaat ctagcgcttc gtcaggagtc aaggcatctg agatatagcg
       61 gaaatcgata caggagacaa gctgctccgg cgtcatatcg ctgagcagtt tccaaactgg
      121 cttgccctcg accttggccc agagatccca gaccgcgttg accaccgcgg cggtggccat
      181 atgaatggct cccttctccg gcccgatcca gcgcagctgg ctatcgcctg taatatgccg
      241 ccagaaggcc cgcatatcag aggtgaacga ctccaggctt cgcccaataa caagcggctc
      301 cagtgcccag atggcagcga cgcagacttc attgccacgc cctatagtga aggacaggcc
      361 atgcccttcc aggttcgccg gatgatcggt ctgcagaacc acgtaggccg cggaataatc
      421 cgggtcgtta ttcatggcat cagaaccggc cagggttcgc gacgttggga aacgaatgtc
      481 gtatgccata aagccggtaa ttgtgatggg catgttttct tctcctgtta agcgatactc
      541 ctccaggtcg agttagctca acgctacaag gcggtcccat tctcctcaaa gatatcagcc
      601 aggacattgg ccatcgcgtg agagatatgc gcggctagca actctgctgc tttttcaatg
      661 tttcccgcga gcagagctac gaggatctgc tcatgatcgt cgagtgctgc ctccatacgc
      721 tgtgggtaca gcgtgtcctt tatctggaat acactgaact ggctgcggag tgtggagaga
      781 taatgaataa gccggctatt gcccgaggca cgaatgagta gtgtatggag gcggaagtcg
      841 cactgcagaa aaagcgatat aggtcgctgg tctagttgct cgcgcacctt gtagaggaga
      901 tcaagctgcg atctgagatc cgcggggtcg agccggggag cagccaggcg caaagccaga
      961 acttccagtg ccccacgcag ctcgtagatt tcctggatat cttgagcggt aaacaggcgt
     1021 acccagaaac cgcgccgtgg gtcagaacgc accagcccat cctgaaccag ccgctctaac
     1081 gcctggcgaa ctggagttcg gctcacacct aacttttcag cgagagcgac atcggagagc
     1141 ctttcctctt tgcgttgatt agagaatacc agttgcttcc agatgagttc gtaagcatag
     1201 tccaccgccg tgggaatggc attgcggcca tgcccccaca gcagttcaaa tacctcgtcg
     1261 ctgaaatctt cgtcaattac ttgagcattc gagtctactc ctacctcgcc tggttcaact
     1321 ggtggttttg tcttcatcgc ctctccattc ttcatctgga aggatagatg cacatgctcg
     1381 ctacagagcc aattcgtggc tcatattata aactgaagtt tcttagagag caattttcct
     1441 tctgcctctt gacttttgac cgtgcataat gtattctaaa ttttgtatac agtatactat
     1501 tatagtattt ttgccagaac ttgtcaagaa gaggaggaaa ccatgaattc gaagttcgta
     1561 agtcgtctgc gagcgctcag tgcgctcgtc gtcattgcga cagtcctggt ggcgtgcggt
     1621 ggcacgtcag gcactacatc gacgtcaggc acctcaacga catcaggagg tccgctcgca
     1681 tcgggagcaa gcttcaagct cggtgtttcg ctgaccttca acaacaccga cttctggacc
     1741 aactacatct cgtatgagca aaagttcgcg agccagtaca atgccacact gatcgggcca
     1801 ctggttgcca ataatgatgc cgccaaacag atcacggaca tccacacgtt gatccaggaa
     1861 ggcgcacagg cgctcatcgt caacccggta gacagcgctg cgatcgcgcc cgcgctcgat
     1921 tacgccgcca gcaagaatgt cccggtggtc agcgtggacg tcgcgcctag cagcggcaag
     1981 gttttcatga tcgtgcgcgc cgataacgtg ctgtatggcc agaactcctg caagttcatt
     2041 gcctcacatg cgactggcac cagcggccac atcgcaatcc tggaggggga tctcgcctca
     2101 ctgaacggta aggatcgcac agatggctgc aatcaagtca tccaacagaa ctatccgaac
     2161 ttcaagaccg tcgagtacgc aaccaagtgg gataccccga cagccgttaa cgatgccaaa
     2221 acggcgctga gcacgtaccc ggacctcagg ggcatctatg tcgagtgggg cgcgccagag
     2281 gacggcattc tcgcggctga gcaagccgct ggcaagttta ccccggtggg cagttcatcg
     2341 cacattgtcc tggttggaaa tgatggcgtg ccacatgaac acgcgctgat ccgttctagc
     2401 aagctcgatg cgaccatttc gcagccggcc aactcctacg cgcagtatgc agtcttctat
     2461 gcacgggctg ctctcgaggg gaaaacctat gcagcgggcc agacaacaga ccacaacagc
     2521 acgtatgtca acctgcaagg caaccttgag gacgcgctgc ctgctcctgt tgtggacagc
     2581 accaacgtca atgacccgaa cctttggggc aattcgaagt ctggctccta acaacgtcgc
     2641 gcccgggacc gtaccggaac ctgtgaacgg aggatggaaa tcgtgctgga acctgtgaac
     2701 ggagggccga ccggagcccg gctctccgtt ggtgtggcgc agagcgtctg gaaagccttc
     2761 ggcgaaaccc aggcgcttcg cgacgtatca ctcgaagtcg aggctggtga atgccacggc
     2821 ctggttgggc gcaatggcgc agggaaatcc accctggtgg cgatcttcac tggcttgatc
     2881 cggccagatc gcggcgttgt caggcttgga gccgaggccg cgccttccct ggctgaccgc
     2941 gccgcctggc agcgcctggt cgcgtgcgtc taccagaagt cgatggttgt tcccaccctt
     3001 acagtcgccg agaatatttt cctcaatcgc gctaccgggg atgaccacgg tatcgtcaac
     3061 tggcgtgccg tgcgatcccg tgcgcgccag gtcatgctcg actggggctt cgacctggac
     3121 gtcaaccgcc tcgcacggga cctcactgtt gagcagcgcc aggtggtcga aatcgcccgc
     3181 gccctttcag tcggtgcgcg cttcctgatt ctcgatgagc ctacagcatc gctggagaag
     3241 gctgccatcg aacgcctatt tgaaagaatt cgcaggctca aagaaagtgc agttggtatc
     3301 ctctatatct cccatcacct ggaggagatc tacgaggtat gtgatcgagt caccgtgctg
     3361 cgcgacggcg agcgtgtgtt gagcactccg gtcagcgaga ttgagcagag ccgcctggtc
     3421 gaggcaatgg ttggggtcgc cccgcaacgg gtaacggaga tcgttgcccc gctgcgagtc
     3481 attgcttcga ctcgaccgag gctcgaaatt tcacacctca atgtcccggc cacgttgggc
     3541 cctgtgcagg acgtgagctt ttccgtgagc tcgggcgagt gcgtcggcct ggttggattg
     3601 cagggctccg gaacggctac cgttgctgac gctgtggtcg ggctggtgaa gccgctatcg
     3661 ggcgagatca agctggatgg caaacctatc cgcacgggaa aggttgatgc aaccttccgg
     3721 caaggtgtaa catatgtacc tgaggacagg catgcgcgtg gcttcgttcc ttacctcggg
     3781 gtaggcgaga acttgacgtt atatatcctc gaccgcctat cgcactgggg gattgtttcg
     3841 cgttcaggcc gtgatcgagt cgcgctcgac ctggtgaatc agttaggtat cgtcaccagt
     3901 ggccttaatc agcaagttgg gcagttgagt ggtgggaacc agcaaaaggt cgttgtcggg
     3961 cgagctctcg cccagcaccc tgccgtgctg gtcgcggttg cgccgacggt cggcgttgat
     4021 gtggctgcca aggagtcgct cctcaacgtg attggggaag cgcgcgatgg tggtactgcg
     4081 gtattgttgg tctccgacga cctggaggac ctgcgcatct gcacgcgact gctcgtcatg
     4141 gtcaagagta gactggtcaa ggagtaccgc cagccaccgt gggacaggca tgaattgatc
     4201 gccgtggtcg agggcctcga tccggcctcc atacgaggga ataacggcat gagtaaggga
     4261 gattcggaat gagtaagtca acaatggatc gtccggtcga ccgggcaggg cagacgctcg
     4321 ccgggacact gcggcgtcag ggcaggcacc ttatagccaa ctggatccgg gaactcgcgc
     4381 tgcttccggt gatcgtcctt ctcctggtcg tcggctcctt tgtgaatccg gccttcttta
     4441 cctcagataa cttgatcaac gtcgcgcaac aatccgctgc attaggtgcc gtggtagtgg
     4501 ccgagaccct gatcctgctg accggcaaat ttgatctgtc ccttcaagcg acgtacggcc
     4561 tggcgccgat ggtgggagcg tggttgatcg tctctaagga cggccagggc ctgggcacca
     4621 actggaaccc gtttatcggc atcgcgatcg tcctgctagt tggacttgtc gtcggagcct
     4681 ttaatggctt catggttatc aagctgggct tcaacgcctt catcttcacg ctggccgtgc
     4741 tgatcctgct gaccggcatt caggtgggaa tcgtcagtgg acgcactatt taccatctgc
     4801 ccgacgcgta catctatctg ggctcgcagt atctccttgg tgtgcccgtc tctgtttggg
     4861 tcaccggtgc gatcttcttg ctctctgcgc tcttcctgag gtatcaccgg attggacgcg
     4921 ctatctacgc catcggcggt aacacagaag cggcccgtgc tgcgggcatc cacgtggacc
     4981 gggtccgcat tggcgttttc atcgtcgcca gcatcctggc cgcgcttgcc ggcctgatga
     5041 ccgctggaca ggtagtagcg gtcaccgcca gccagggtaa taacctcatc ttccaggtct
     5101 tcgcggctgc tgtcattggc gggatatcac tcgaaggcgg acgtgggcgt atgcttggcg
     5161 cgctgaccgg cgtcatcttg ctggcgctgg tcacaaacat cctgatccta tcgcagatcc
     5221 agacgttctg gatcgacgcc gccaacggcc tcatcatctt aattgcgctg gggctggccc
     5281 ggttgatcgg cagcgagcgc tcctagagtg caccaagcta tgcgcgaaca ggcaactgct
     5341 gtaatgacca gccattccca tcgggatcgc taaaaaagac aaatgagccc cagggcatta
     5401 cttcgatctc actggcttca acgcctcgtt ttaccagttc gtcgcgtact tctttaacgt
     5461 ccggtaccac catttgaaga cctttgactg atcccggctg catgtctgta atgccaaccc
     5521 caataactat cgagcaggcg gaaccgggag gtgtcaactg gacgaatcga aggtcatccc
     5581 tgacctggta atcatgatca gcattaaagc cgatcttctc cgtataaaaa gcctttgcgc
     5641 ggtcaacatc cgatacggga accgctacta gttctaattt catttgcata cgggaattat
     5701 atgaaaaggt aatgctggat gtcaatgtga tgacagccta gcagcgccca ggaactttta
     5761 aaccgtagga gtaaatattc gggtacactt aggatatgta attcggctcc acaaggggcg
     5821 ccaatggcaa tttactatta gacaaggttt atttatttaa atgattcgtc cacattgaac
     5881 cttgctttag ttaaaattgt tccaatagaa aatctgagct cttttaaaaa tc
//
LOCUS       DASZAK010001381         2759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_141574, whole genome shotgun sequence.
ACCESSION   DASZAK010001381 DASZAK010000000
VERSION     DASZAK010001381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141574"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            429..1856
                     /locus_tag="VGT44_24220"
     CDS             429..1856
                     /locus_tag="VGT44_24220"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEV2583983.1"
                     /translation="MINEQTGEPNAVIATEVVRKSSSLGLSLEGMHGTVAVPHHQAGF
                     WQQWRAFVGPAILVSVGYMDPGNWGTDLAAGAQFKYGLLWVVGFASLMAIFMQVIAAR
                     LGVVTGKDLAQCCRDWYPSWTRKPNWLFCELAIGACDLAEVLGSAVALNLLFGIPLLW
                     AVIITGLDVLLLLGLQRFGVRTIEAVVLLLVITIGVCYFIEIFILPQTRPSFAEMAAS
                     LVHPGLGQNGMMFLAIGIIGATVMPHNLYLHSALVQSRIMQKDEPSVRSGILFNTIDA
                     TAALTIAFFVNAAILVLAALVFYGKASVTVPGGQLVTFSPQSDWIRVAFLTLAPLLGT
                     SLAGTLFAVALLASGQSSTITGTLAGQVVMEGFMHWRIRPWLRRLITRTLAIIPAVVV
                     IGIRGNSSVTDLLVLSQVVLALQLPFAMFPLLQFTSSRKRMGKWRNGWFLMMTGWGSA
                     GLITAMDIYSLPDAMKQAWTIIAGH"
     gene            1992..2408
                     /locus_tag="VGT44_24225"
     CDS             1992..2408
                     /locus_tag="VGT44_24225"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV2583984.1"
                     /translation="MYNKILVTLDATPTDRAIVEHIKSLAKIMHSTIVLLHVADGWAA
                     RMYGADAVSPEVTQDKAYLEALRAEFQSEGIPAEAELAFGEPTDEIVKWIQEKGCDLV
                     AMSTHGHRFLGDLLLGTTAPNVQHKVKVPVLLLKAK"
BASE COUNT          578 a          755 c          812 g          614 t
ORIGIN      
        1 caacttgccg tccgtccagg atgcgtcctg ggccaagcaa atagaagctc aattacatgc
       61 ccttgatcga aggtcgtaat cattcgcctt ggcacgcctt gaccatgaac agttcgatac
      121 cgtaatctga aacatgacaa acatccatgg gaagattaag gcgggtcgag atcatgtcaa
      181 tcgaggccta ttgtcgcgat ttggaacttc gtccgatgga tgctccacac ctattggctg
      241 gttcttcgaa gcgcgggagc atgctcccgc tttgtcgggt ggagccttgc ttccgcagaa
      301 tcacaatgat cgggcagaac tagccgcgcg tatactcgca gccagcctat gttaattgag
      361 ttccaaatca tgcctctcga acattctcaa acttaaaagg cgtgtcagtg gaaaaaccag
      421 ggccacattt gataaacgaa caaactggag aacctaatgc agtaatcgca accgaagttg
      481 tccgtaaatc ctccagcctc ggcctttcgc tcgagggcat gcacggcaca gtggccgtgc
      541 cgcaccacca ggctggcttt tggcaacaat ggcgggcttt cgtcggaccg gccattctcg
      601 tcagtgttgg ctacatggac ccgggcaact gggggacgga cctggcagcc ggggcgcagt
      661 tcaagtacgg gctgctgtgg gtggttgggt tcgcgagctt gatggctatc ttcatgcagg
      721 tcatcgctgc ccggctggga gtggtcacgg gcaaggacct tgcgcaatgt tgccgggatt
      781 ggtatccgag ttggacgcgc aagccgaact ggttgttctg cgaacttgcc atcggcgcct
      841 gtgacctggc ggaagtgctg gggagcgccg tggccctcaa tcttttgttt gggattcctc
      901 tgctttgggc agtgatcatc accggactgg acgtgctgct gcttctcggc ttgcagcggt
      961 tcggggtgcg gaccattgag gcggtagtcc tgctcctggt gatcaccata ggtgtgtgtt
     1021 acttcatcga gatttttatc cttccgcaaa cccgtccaag ctttgcagaa atggccgcct
     1081 cgctcgttca tcccggattg ggtcagaacg gcatgatgtt tttggctatt ggcatcatcg
     1141 gcgccaccgt catgccgcat aacctctacc tgcattcggc gctggttcag agccgcatca
     1201 tgcagaaaga tgagccctcc gtccggagtg gaattctctt taacacaatt gatgcgaccg
     1261 cggccctgac cattgctttc ttcgtgaacg cagccattct ggtgcttgcc gcgctggtgt
     1321 tctacggaaa ggcgagtgtg actgtccccg gcgggcaact ggtgacgttt agcccccaaa
     1381 gcgactggat ccgggttgcc ttcctgaccc tggccccgct tctgggaact tccctcgccg
     1441 gcacgttgtt cgccgtcgcc cttctggcca gcggacagag cagcaccatc actggaacat
     1501 tggcaggaca agtggtgatg gaagggttta tgcactggcg aatccgcccc tggttgcggc
     1561 ggcttatcac gcgcaccctg gcgattattc cagcggttgt ggtcatcggg attcgcggca
     1621 acagcagcgt caccgacttg ctggtgctca gccaggtggt cttggccctg caattaccct
     1681 tcgccatgtt tcctctgctt cagtttacaa gctcgcgcaa gcgcatgggg aagtggcgga
     1741 acggctggtt tctaatgatg accggctggg gcagcgccgg gctgatcaca gctatggata
     1801 tttacagcct tccagacgcg atgaagcagg cttggacaat catcgctggg cactgaacgg
     1861 cggcaagcag gatgcgggaa aaggccaact gtgaatgtca tcctgatccc gcgaccgcgg
     1921 gatcagcatg acgagtttac ttattcaggt tcagcattct gctaggggat accttcgcgg
     1981 gagaagacgt tatgtacaac aagatcctgg tgaccctcga cgcgacaccc accgaccggg
     2041 caattgtcga gcacatcaag tcgctggcca aaatcatgca tagcactatc gtgctcttgc
     2101 atgttgccga cggctgggcg gcacgcatgt acggtgctga cgccgtgagt cccgaggtga
     2161 cacaggacaa agcctacctg gaggcgcttc gggcggagtt ccaatctgaa ggcattcccg
     2221 ccgaagccga attggcattc ggcgaaccca ctgacgaaat cgtcaaatgg attcaggaga
     2281 agggttgcga cctcgtggcc atgagcaccc acgggcaccg gttcctgggc gatctcttgc
     2341 ttgggaccac cgcccccaac gtccaacata aggtcaaagt accggtgctt ttgctcaaag
     2401 cgaaatgaga ggtctgtcgc gacccttcat tgagcagcga acgtgcgcca tggcgaacag
     2461 gtcatccctc ttcatgtaga accctcttgg ccaagcaacc tccgtagtcc gcagcttgcg
     2521 gttgacacga tagggcagga aaggtaggct tccgggccaa gggaaaggtt aaaacggcga
     2581 ttcccgaata caccaagagt gaggtgtgga gaatatggag acaccaggta tgccggagaa
     2641 tagttcggaa gttgcgtcca ccgtgatgag cggcctggag ggtatcggga tgcctattgc
     2701 attctgcggg tggaggtatc tcgcgccaat ccaaaagtgg gagctagtta tcggaacgg
//
LOCUS       DASZAK010001382         6203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Ktedonobacteraceae bacterium isolate SMAG_U6386
            k141_212882, whole genome shotgun sequence.
ACCESSION   DASZAK010001382 DASZAK010000000
VERSION     DASZAK010001382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37955835
            Sequence Read Archive: SRR12347823
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Ktedonobacteraceae bacterium (soil metagenome)
  ORGANISM  Ktedonobacteraceae bacterium
            Bacteria; Chloroflexota; Ktedonobacteria; Ktedonobacterales;
            Ktedonobacteraceae.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/13/2024 18:45:32
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,850
            CDSs (total)                      :: 4,811
            Genes (coding)                    :: 4,784
            CDSs (with protein)               :: 4,784
            Genes (RNA)                       :: 39
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 30
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 27
            CDSs (without protein)            :: 27
            Pseudo Genes (ambiguous residues) :: 0 of 27
            Pseudo Genes (frameshifted)       :: 5 of 27
            Pseudo Genes (incomplete)         :: 22 of 27
            Pseudo Genes (internal stop)      :: 3 of 27
            Pseudo Genes (multiple problems)  :: 3 of 27
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Ktedonobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212882"
                     /isolate="SMAG_U6386"
                     /isolation_source="soil"
                     /db_xref="taxon:2740538"
                     /environmental_sample
                     /country="China"
                     /lat_lon="27.4448 N 111.353 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..317)
                     /locus_tag="VGT44_24230"
     tRNA            complement(222..317)
                     /locus_tag="VGT44_24230"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(281..283),aa:Sec,seq:tca)
     gene            complement(371..1198)
                     /locus_tag="VGT44_24235"
     CDS             complement(371..1198)
                     /locus_tag="VGT44_24235"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV2583985.1"
                     /translation="MDFRISIIGLLIGFLVGLTGMGGGALLAPILILVLKVPPVWAVG
                     TDNVYSAVTKALGSIVHIRQKNVNLKIAFWLACGSVPATILGVIIVQLIHARLGDAIN
                     SMIVHAVAVTLILVAILMVIKPVLMRRLDRKAMEAQKRAALEGKPQPAEGTSRWEQRY
                     RPFATGLVGAIVGLLVGLTSVGSGTLIMVALAFLYPRLSSKKLVGTDISHAVMLHSAA
                     AIVYFSAGTVNWTIVGLLLLGSLPGVFMGSKLSKFIPDRIMRPILATVLVLSGWKLF"
     gene            complement(1674..4439)
                     /locus_tag="VGT44_24240"
     CDS             complement(1674..4439)
                     /locus_tag="VGT44_24240"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEV2583986.1"
                     /translation="MQSTTPATPAQQVMACPQCDNIVSIYAEFCNICGKRLRPPATGA
                     IPALSSPYALSAQPQDDDDEYEDDDFELEDGETSTTALRVTTATQASSLPQSAGEILT
                     HLRRVQEQIEQMDRYFPAELPGKEQKLVAWKKQIQRALACVELFDRPQLQQSESQQTL
                     KLRHHIAEAARALDFTREYTVKLIGHTGAGKSTLLAALLGQDIFPRLAGGAVTGVCTR
                     VRLCQGQEAEEMQVHFLTRSAFDMLLKKTQQSIKEATSQSAREAMAHELAVLLKAGEA
                     FGEHYLLDRSPRVERIPRERWREESSRYIEEPSRDSEEPRLVRLVDYVEYAVRGGKRA
                     LLPPGSVLVDLPGGSAGQLHHESILHEELSHVDAVILVVGNNRFGDDDRTQRIFELAR
                     RRIAAERSPEVAARMTFLAVTHWDEINSIASLEKALGSMRPLLRDLPTNYSSYHHHGP
                     GNGYFFYPLRALDGLLATLGIEKHRLDADRVQEGRDYAGRILSVYPELLKIDATLPAT
                     ASAQDFQKVSARQHEAMLHFSGLPELTHDLQSFLTTSRYEVQLRQAETQLAMALQQLE
                     DLCWERLNSLGIQSRDLQELELEITMRQSKRGATRFEQIQQRTSSMRDAWKEALKEFD
                     AVIRDEQSAFHTALLTAHDRAARRIKIRIMQGSFDHFIKVGNRVQEDSPALEIGNRWV
                     DIDGWNLIKELRVSFTAALEREMQEPARTLAEAFLLPIAHREEVEGLLDIHSVALGEF
                     GGELDEIQKAYSKLKRAIRDKSRDVCLYVTLGELLNEEKYAPTKDDAAVGALYRLVSI
                     AGKPEDIIQQARQLMGPILDVICSRLAQSTERRIALLFRYELDKLEERQAFESDRLDV
                     PPSSQPGAFTDLINRLYSLLTERVLTSNTLREQLDLLQARREADVDRWIELVHDVEVL
                     KTMHRQG"
     gene            complement(4529..5125)
                     /locus_tag="VGT44_24245"
     CDS             complement(4529..5125)
                     /locus_tag="VGT44_24245"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV2583987.1"
                     /translation="MQQSEDVDPNELSDEALLTAIAGGAVWAMESLYHRYSRILYSLA
                     YRIVADHQIAEDLLQDTFVSVWKRSTTFSPQSGAARNWLISIVHHRAIDHLRRVRRRS
                     VLQEAPLAELDLDEKTAFPDVWDEAWRSVQSSQVRAALMQIPTEQRMVIELAYFQGWT
                     HTEIAEGTQIPLGTVKARMRLGLQHLKRALLEAGMDEI"
     gene            5263..5769
                     /locus_tag="VGT44_24250"
     CDS             5263..5769
                     /locus_tag="VGT44_24250"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEV2583988.1"
                     /translation="MEDSYPSWYPVQIRFRDLDPLAHVNNTVYFTYFEEARSHYFGLL
                     DPWLEQWPSEEEHQHKDEEPQNPRIQTGPRGHRYGMLVKEITCTYELPMVRGDSADVG
                     VRVARIGRTSFIMEHLIRDREVHDRVFATGRSVMVWCNYHTGRPHPVPPSLRAAFEQM
                     EGRMFPIQ"
BASE COUNT         1312 a         1639 c         1835 g         1417 t
ORIGIN      
        1 ccactgatag gattgattgc gagacaataa gactcattcg ttcttcattc tcaatacaat
       61 gctgcatcgg gataggcatg attcgattaa aaaatacttt acgaggtagg gaaaagagaa
      121 gcatgatttt ggagtgccaa atgggaagca taggttttgg aaatctccaa aacctatagg
      181 taaagtgata caggcccaca caaagatctc acgcgagtgg gcagaggcgt atgggagtcg
      241 aacccaccgg ggaccgcgcc gagcgacccc ccagccgttt tgaagacggt gagacccacc
      301 ggagtcccta cacctccatc gtaacagggt gtattttgtc atgcgtggcc gggcttgtca
      361 agccgatgtg ttagaagagt ttccagccgg atagcaccag cacagttgca agaatgggac
      421 gcatgattcg atctggaata aacttgctca gtttgctgcc catgaagaca ccgggcaggg
      481 agcctagcag caacaatccc acgatggtcc agttgacggt gcctgcgctg aagtagacga
      541 tggcggcggc tgagtgcagc atgacggcgt gggagatatc ggtgccgacc agcttcttag
      601 aagacaggcg ggggtagagg aacgccaggg caaccataat caatgtgccg ctgccgaccg
      661 aggtgagacc cacgagcaag ccgacgattg cgccaaccag gccggtggcg aaggggcgat
      721 aacgctgctc ccagcgcgat gtcccctcgg ctggttgggg tttgccttcg agcgccgccc
      781 gtttctgcgc ctccatagct ttgcgatcca ggcggcgcat caagactggc ttgatcacca
      841 tcaagatagc caccaggatc aacgtgacag cgacagcgtg tacgatcatg ctattgatgg
      901 catcgccaag tcgcgcgtga atgagctgga cgatgatcac acccaggatc gtcgctggta
      961 cgctgccaca ggcaagccag aacgctatct tcagattgac atttttctgg cggatatgca
     1021 cgatcgaacc aagcgctttt gtaacagcag agtacacgtt atcggttcca actgcccaga
     1081 ctggcggaac tttcaagacg agaatcagaa tgggcgctag caatgcacct ccacccatgc
     1141 ccgtcaggcc caccagaaag ccgatcaaca ggccgataat cgaaatgcga aaatccacta
     1201 tgtcacctct gaaaaatcta tattacgttt cgtgagatgt tactttcagt gagaaatgta
     1261 actgacgttt catttctgtg ttaagaatat catgctttat cgatgaggtc tagtgagaag
     1321 gggttaagat tttgagcttc acggtatatt tttcttaacc ctgcagcgcc gggaggatga
     1381 tctggctcaa gacgtggggg cctgtgggtt tcttaacctc aatggcaaga tatcgcataa
     1441 atcttcatcg attctcaaca ggttgtcaac ctcattgcaa ccgtgcaagc atattattat
     1501 ttccaggggg aaagccacat gggggatgtc ctggggggga catatagtgg ctcaacacac
     1561 gggtcattta aactggggga caggaaggat gatcgatttc cttctccttc ctgtcagaaa
     1621 gcggaaagcg gttttatcaa ctgctttccg ctttctgttt ttggagcaag cttttatccc
     1681 tggcggtgca tcgtcttcaa tacttcgaca tcatggacca gttcgatcca tcggtcaaca
     1741 tcggcttcgc gccgggcctg gagcaagtcg agttgctctc gcagggtatt ggaggttaga
     1801 acacgctcgg tgagcaggct ataaaggcgg tttatcagat cggtgaaggc gccaggctgg
     1861 ctggagggcg gcacatccag gcgatcgctt tcaaatgcct gccgctcttc caacttgtcg
     1921 agttcatagc ggaacagaag agcgatgcgg cgttccgtgc tttgcgccag ccggctacag
     1981 atcacatcaa ggattggccc catcaattgg cgggcctgct gaatgatgtc ctccggcttt
     2041 cctgcgatgg aaacgaggcg atagagcgcg ccaacggcag cgtcatcctt tgtcggggcg
     2101 tatttctctt cattgagcag ttcgcccagc gtcacgtaga ggcagacatc acgcgatttg
     2161 tcacggatgg cgcgtttcag tttgctatat gccttttgaa tttcgtcgag ttccccgccg
     2221 aattcgccca gcgcgacact atgtatatcg agcaagcctt ccacctcttc ccggtgcgcg
     2281 atgggcagca gaaaagcctc ggccagtgtg cgggcaggct cttgcatctc tcgttcgagc
     2341 gcggcagtga agctgacacg tagttctttg atcaggttcc agccgtcgat atctacccag
     2401 cggttgccga tttccagtgc gggcgaatct tcctgcacgc ggttgccgac cttgatgaag
     2461 tggtcgaagc tgccctgcat gatgcggatt ttgatgcgcc tcgcggctcg gtcatgcgcg
     2521 gtgagcagcg cggtatggaa ggcgctttgt tcgtcgcgaa tgacggcgtc aaattctttg
     2581 agcgcctctt tccaggcgtc acgcatcgaa ctggtgcgct gctgaatttg ctcgaagcgc
     2641 gtcgcgccgc gtttgctctg gcgcatagtg atttcaagtt ccagttcttg taagtcgcgg
     2701 ctctggatgc cgagcgagtt caggcgttcc caacagaggt cttccaattg ctgcaaggcc
     2761 atcgccagtt gcgtttcggc ctggcgcagt tgtacttcgt agcggctcgt cgtcaggaag
     2821 ctttgcaggt cgtgcgtcaa ttcgggcagg ccgctgaaat gcagcatggc ctcgtgctgg
     2881 cgagcgctga ctttctggaa atcctgcgca gatgcagtcg caggcagggt cgcatcgatt
     2941 ttgagcagtt cggggtagac gctcaagatg cgcccggcat agtcgcgtcc ctcctgtacc
     3001 cggtcggcat cgagcctgtg cttttcgatg cccagtgtag ccagcaggcc atcgagggcg
     3061 cgcaggggat agaagaaata gccgttgccc ggaccatgat ggtgataact gctgtagttg
     3121 gtcggcagat cgcgcagcag ggggcgcatg ctccccaggg ctttttccag gcttgctatc
     3181 gagttgatct catcccaatg cgtgactgcc aggaaggtca tgcgcgcggc aacctccggc
     3241 gagcgttccg ccgcgatgcg gcggcgcgcc aactcgaaga tgcgctgtgt gcggtcatcg
     3301 tcgccgaagc gattattgcc cactaccagg attacggcat cgacgtggct gagttcctcg
     3361 tggaggatag actcgtggtg cagttggccg gctgacccgc caggaaggtc aaccagcacg
     3421 ctgcctggcg gcagcagggc acgtttgccg ccgcgcactg catattcgac ataatcgacc
     3481 aggcgcacca ggcgcggctc ttcgctatcg cgcgacggtt cctcgatata gcggctgctt
     3541 tcttcgcgcc agcgctcgcg cgggatgcgt tcaacgcgcg gtgatctatc cagcaggtag
     3601 tgctcgccaa aagcttcgcc tgccttgagc agtaccgcaa gctcgtgtgc catggcctcg
     3661 cgcgcgcttt gacttgtggc ttctttgatg ctctgctggg tcttcttcag cagcatgtca
     3721 aaggctgagc gagtcaggaa atgcacttgc atctcttctg cttcctgccc ctggcagagt
     3781 cgcacccgcg tacatacgcc ggtgacggct cccccggcca ggcgcggaaa gatatcctgg
     3841 cctagcaggg cagccagcag cgtgcttttg cccgccccgg tatgaccaat cagtttgacg
     3901 gtgtactcgc gcgtgaagtc gagggcgcgg gcagcttcgg cgatgtgatg gcgcagcttg
     3961 agcgtctgct gcgactcgct ttgttgcagt tgcgggcgat cgaagagttc gacgcaggcc
     4021 agcgcgcgct gtatttgctt cttccaggcc acaagcttct gctccttgcc tggcagctcg
     4081 gcagggaagt agcgatccat ctgctcgatc tgctcttgca cacggcgcag gtgcgtcaga
     4141 atttcgccgg ctgactgtgg gagcgaactc gcctgcgttg ctgttgtgac gcgcaaggcg
     4201 gtcgtgctgg tctctccgtc ttcaagctcg aagtcgtcgt cctcgtattc atcatcatcg
     4261 tcctgtggtt gggcggaaag ggcatatgga gatgaaagag ctggtattgc gcctgtagca
     4321 ggaggacgca agcgttttcc gcaaatattg cagaactcgg catagatact gacgatgtta
     4381 tcgcattgtg gacaggccat cacttgctgc gcaggcgttg cgggcgtggt agattgcatc
     4441 ccaggaacgt tcccccttta tggaaatctc gcttacccag cgatgatagc atagaggccg
     4501 cttgcggggc aagcggatgt gacttttgtt agatttcgtc catgccggct tcgagcagag
     4561 cgcgtttgag atgttgcaaa ccaagcctca tgcgggcctt gacggttccc aggggaattt
     4621 gtgtgccttc cgcgatttcg gtgtgcgtcc agccctgaaa ataggccagt tcgatgacca
     4681 tgcgctgttc tgtaggaatt tgcatgaggg ctgcgcgcac ctggctgctt tgcacggaac
     4741 gccatgcctc gtcccaaaca tccgggaagg ccgtcttttc gtccaggtcg agttcggcca
     4801 ggggcgcttc ctggaggaca gagcggcggc gcacacggcg caggtgatcg atggcgcggt
     4861 gatggactat ggagatcagc cagttgcgag ccgcgccgga ttggggtgaa aaggtagtgg
     4921 agcgtttcca gacggagacg aatgtgtctt gcagcaagtc ttcggcgatc tggtggtcgg
     4981 cgacgatgcg atacgccagc gaatacagga tgcggctata acgatggtat aacgattcca
     5041 tggcccagac tgctcccccg gcaatagcgg tgaggagggc ttcatcgctc aattcgttcg
     5101 ggtcaacatc ctctgattgc tgcatcgctg ccttctttat tttgttatta ctctagtcta
     5161 cgatatccga aacgaattag atcagtccat ttggcaagag tgaagagatt gtaatatgct
     5221 gtttctaaca tatccgtgta gaaacagaaa ggaacgctta caatggagga ctcctaccca
     5281 agctggtacc cggttcagat tcgttttcgc gatcttgatc cgctggccca cgtcaacaac
     5341 accgtgtact ttacctattt cgaagaggcc agaagtcact atttcggcct gctcgatccc
     5401 tggcttgaac agtggccatc ggaggaggaa caccagcaca aagacgagga gccgcagaat
     5461 ccgcgcattc agacggggcc gcgcgggcat cgctacggga tgctggtgaa agagattacc
     5521 tgcacctacg aactgccgat ggtgcgcggc gatagcgccg acgtgggtgt gcgcgtggcg
     5581 aggattggcc gcaccagctt catcatggaa cacctgatac gcgaccgtga agtgcatgat
     5641 cgtgtcttcg cgactggccg gtcggtgatg gtctggtgca attaccacac cggtcgtcca
     5701 caccctgtgc cgccatcgct gcgcgctgcc tttgaacaga tggaaggaag gatgttcccg
     5761 atacaatgag aacgtgaaag caaaaagaac atcgaaaaaa ttcgttgaaa aaaggtaatc
     5821 caaaatgagc gggctggcgt agtacgtata tatcaggttg ccgagggggg aatgacgagc
     5881 gatacggcgt tagcggcgat tgctccatgt cgcgtcagtc ggtttgttcg tccttgcaat
     5941 ctatggagca atcctttctg taattatttt cacttctcct tctctggttc ccaggaggca
     6001 ctgatcgaaa gatggggtaa cgatcagtgc ctctctcttt ggctaaaaaa gtttcccctg
     6061 ctctacctga tcctccggat catgcctgtc ccagggcagc gtggggagcg cggcaggcgg
     6121 taagcggcgg cgcacacgtg caaagagcgc gtgacagatg tcgggtgagt gaacctcgaa
     6181 tgggcagtgg cagaagatga aaa
//
